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Archive for
Home of the British NERF Community
BRIT NERF Forum Index
Nerf Wars
Super Stock / NIC WAR
Hi Enthusiasts!
I'm sure i'm not the only person who has gone abit excessive on one of their blasters to the point that no-one really wants to let you play with them.
I'd like to propose a super stock NIC style war with highly modded blasters (within reason no 300+ FPS hydro cannons)
If there is enough Interest I will organise something before Christmas if not I will get something going in the new year.
Last war in Hendon there was a good turn out with alot of people talking of further modding their arsenal with the goal of producing some super stock type blasters.
If anyone is interested please add to this post or send me a pm.
I will put together a facebook group in a week or two after i've seen the interest to get going.
I'm London based and always happy for a game if your interested.
This will be a London based event, most likely taking place in a field / forest environment.
If turn out is high and poeple don't mind to spend abit of money i've spoken to a paintball arena who said they would be happy for us to take our Nerf blasters and play with them for a fee ofcourse.
I will get more details pending relevant interest.
Protective eyewear will be MANDATORY.
Just to clarify - 'super stock' = taking something Hasbro have produced and maximising it's potential. 'NIC' = taking some plumbing supplies and making a single shot drain blaster style affair.
Think there might be some mixing of metaphors?
Seeing as we were playing with people running tri-hellcat rapid pistols at the weekend i think we are already pushing where super stock can go.
If you want to do NIC then that would be a different matter, but i'd still be sticking an fps limit on the plan.
Regardless of the type of event good luck with the planning - hope it goes ahead for the southerners benefit!
Hey there fella.
Always happy to see a new face organising an event. You've been to Hendon so you already know what to expect at an event and you already know what sort of turnout to expect. Put your thinking cap on and come up with a firm plan (baring in mind the winter is fast approaching). Once you have the where/when/why you can start recruiting.
Always good to have a new event in London. Hopefully I'll see you there.
Both of you raise some very good points.
Forgive my terminology but i mean high powered blastetrs.
I would say an FPS limit of 300fps will be the target.
Also please forgive me if I may have put the post in the wrong place but I want to gain an understanding in the interest of high power wars as until now i have only seen very low power wars with around 120 fps limits im thinking for a chance to pull out our longshots / drain blasters within reason.
If you guys are positive that this is something that will go down well I'll make a facebook group and a temporary war date as a clearer point of call.
Super stock =! NIC. NIC is plumbing pipe, single shot, speed ball style set ups as per the US. Super stock is 100FPS + and generally tapers out at the point where we stop being able to push stock blasters. That's probably ~140FPS at the moment with the optimum Artefact red cage and wheels.
In terms of actual players, you'll still get most of us rocking up with our sub 130FPS hardware - ballistic limits don't bother most players for a variety of reasons. For a start, the majority of builds are under even the standard 130FPS cap. As a proportion of players, the modding community is pretty small. Heck, I can think of one UK player who has a complete load out that could be designated remotely NIC. Everyone else will just turn up with what they have and the occasional higher powered blaster might make its appearance. There's a whole bunch of reasons why the envelope isn't getting pushed in the higher FPS direction. For example, higher FPS isn't really going to help you if you can only manage one round a second, someone with an RS or Stryfe is going to close with you while you're reloading and put up an escapable wall of foam.
Nerf benefits from the fact it's close in, rapidly changing game using lower blaster energies and more human energies. Anything that gets bogged down in pitched exchanges is dull and not why we Nerf. If I wanted that, I'd go and play air soft or paint ball.
Higher ballistics also require non-stock ammo to be even remotely worthwhile. That's a whole nest of vipers you'll have to contend with: the safety factors around home-made ammunition. Any war that allows non-OEM ammo is a non-starter for me.
If you want to run a "if it's not an air weapon and can fire Nerf" game then fine, make sure it's 18+ and on private land so you don't have problems with shooting passers by. For the UK current ballistic limits are ~170FPS FA and ~250FPS semi-auto/single shot before you start having to register them as air weapons (that's based on a 1g Elite dart, other ammo types will vary). The last thing the community needs is plod and the courts breathing down our necks because a random dog walker got shot with a drain blaster.
In terms of event logistics and game design, build a normal Nerf war that allows higher powered blasters and build in safety protocols accordingly. You could advertise the no-holds-barred style as a selling point but don't make it the focus. It won't change the fact most people will turn up with what they have and that way you don't risk alienating anyone who looks at the ad and thinks "well, fuck that I'll be out gunned so there's no point". The latter is a problem that NIC groups like SENC in the US regularly report.
BRIT NERF Forum Index
Nerf Wars


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Long considered one of the world's premier automakers, Ferrari is celebrating its 70th anniversary this year. Of course, any auto aficionado worth his or her salt is desperately searching for a way to take part in this milestone.
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Standard Vanguard III
|Vanguard III
1957 Standard Vanguard III
En flot Standard Vanguard Fase III med omfattende dokumenteret historie. Bilen har en 2,1 liters motor med overdrive. Bilen har ingen kosmetiske eller mekaniske problemer og starter, kører og stopper som den skal. Dette er en bil, der kan køres i mange år fremover. Bilen har danske papirer og registrering der dateres tilbage til 13. april 1957.
Bilens specifikationer:
Speedometer: 9524
Stel: V323543LDL
1. Registeret: 13 april 1957
Motornummer: V324551E
Motor: 4 cylindere, 2088cc motor med sin single Solex downdraught-karburator der producerer 68 bhk
History of the Standard Vanguard Phase III (1955–1958):
The Standard Motor Company Limited was a motor vehicle manufacturer, founded in Coventry, England, in 1903 by Reginald Walter Maudslay (1871–1934). It purchased Triumph in 1945 and in 1959 officially changed its name to Standard-Triumph International and began to put the Triumph brand name on all its products.
The Phase III, released to the market for the mid-October 1955 Earls Court Motor Show, was a radical change with the elimination of the separate chassis. The 2088 cc engine with its single Solex downdraught carburetor now produced 68 bhp (51 kW; 69 PS).
The front suspension was independent, using coil springs, and was bolted to a substantial sub-frame which also carried the recirculating ball steering gear. Semi-elliptic leaf springs were used on the rear axle. Lockheed hydraulic brakes with 9 in (229 mm) drums were fitted front and rear. The three-speed gearbox had a column change and the optional overdrive was operated by a switch on the steering column. A four-speed floor change became an option.
The new body was lower and had an increased glass area, making it look much more modern, and the old two-piece flat windscreen gave way to a one-piece curved design. The wheelbase increased by 8 in (203 mm), giving much better passenger accommodation. A heater was now a standard fitting. Bench seats were fitted in front and rear with folding center arm rests.


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There are total 14 letters in Ethnologically, Starting with E and ending with Y.
Total 1184 words created by multiple letters combination of Ethnologically in English Dictionary.
Words that starting with Ethnologically
Words that containing Ethnologically
Words that ending with Ethnologically
Definition of the word Ethnologically, Meaning of Ethnologically word :
adv. - In an ethnological manner, by ethnological classification, as, one belonging ethnologically to an African race.


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Skipper is the leader of a band of penguins bent on a secret agenda of escaping the confines of the zoo and being free in Antarctica. He is the second shortest and chubbiest of the group (Only Private is shorter and chubbier) and he has ice blue eyes. It is probable that Skipper and the rest of his squad are warm climate penguins, for they do not live in an air-conditioned habitat. This is supported in one episode, where the city was having one of the coldest days ever, and the penguins used heat lamps to warm up their habitat, and also in the movie where the penguins aren't bothered by the warmth of Africa and Madagascar, but intensely dislike Antarctica.
| Something seems..."broken"
This article is a stub. If you're an administrator, you can help by extending it.


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How did I get here?
I'm not winking – I'm contemplating with one eye closed.
The end result is the same: I'm a beautiful kitty and my humans love me!
Quotes of the Day
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A good traveller is one who knows how to travel with the mind. (Michael Bassey Johnson)
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Title quote and first line are from The Talking Heads, "Once in a Lifetime."


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Barbecued Chicken Breasts
Be the first to review
READY IN: 35mins
Recipe by internetnut
I found this recipe in O The Oprah magazine. I have not tried this recipe, but I'm posting it for safe keeping.
See Discussion:
Ingredients Nutrition
- 1 1⁄2 cups bottled reduced sodium Italian salad dressing
- 1⁄2 cup ketchup
- 2 1⁄2 tablespoons reduced sodium soy sauce
- 2 tablespoons minced garlic
- 6 boneless skinless chicken breast halves (1 1/2 pounds)
Directions See How It's Made
- In a bowl, mix dressing, ketchup, soy sauce, and garlic. Add chicken. Stir to coat and cover with plastic wrap. Refrigerate 3 hours.
- Preheat oven to 400. Arrange chicken breasts on a rimmed baking sheet lined with foil; pour marinade on top. Roast chicken 20 minutes or until cooked through and no longer pink, turning twice during baking. Remove and serve.


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woke up shallow breaths
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text: Almost half of us experience a 'life crisis' Thursday 21 April 2016 PDF Print UNDER EMBARGO UNTIL 22.04.16: 00.00 Almost half of us experience a 'life crisis' The Open University urges us to pursue our dreams · Unfulfilled dreams and ambitions top list of 'life crisis' causes · A fulfilling career and taking up interests key to overcoming 'life crisis' London, 22 April 2016: Almost half (44%) of the British public have either had or are going through a 'life crisis', a poll recently commissioned by The Open University reveals today. To help people restore their personal balance, The Open University is urging people to discover their 'Plan P - their 'Passion Plan' - and realise their unfulfilled ambitions. However, it's not just those midway through their lives who have suffered a 'life crisis' and need to re-ignite their passions. Almost a third of those surveyed (29%) have been through a 'life crisis' between the ages of 18 and 30, suggesting millennials are particularly susceptible. When asked what factors caused their 'life crisis', a lack of career fulfilment and unfulfilled dreams topped the list. To combat this, 39% said embarking on a new career would help solve their issues and 24% said learning something new would have the same effect. Whilst over two thirds of those surveyed wish they spent more time pursuing their personal passions, 27% don't think they have time to do so, with long hours of work and social pressures swallowing up free time. One in ten of those surveyed do not have any personal passions or interests outside of their career, however 41% said that taking up a new interest or hobby would help address their 'life crisis'. As a result, The Open University is today urging people to explore their interests by learning something new or pursuing further study in order to address the issues associated with their 'life crises', start realising their ambitions and to discover their 'Plan P'. Clare Riding, Head of Careers and Employability Services at The Open University said: "Almost two fifths (39%) of people cited embarking on a new career as a solution to their 'life crisis' so whilst finding a career you love can be challenging, it is also deeply rewarding. Taking time to explore your interests, both in and out of work, will help you to realise your career ambitions and will support you in finding the role that's right for you." "Hockey is a real passion of mine," Helen Richardson-Walsh, Olympic hockey player and OU student commented, "But it's not my only interest in life. 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"The Open University changed my life," Victoria Ann Trull, trainee teacher and OU graduate said, "I didn't choose the right degree when I left school but having some more time, and getting more life experience, helped me to better understand what I wanted to do. Thanks to The Open University, I was able to study something I'm really passionate about - physics - and I can't wait to start teaching this full time." - ENDS - For further information, please contact: Lexis Emily Tanner: firstname.lastname@example.com 020 7908 6406 Laura Bates: email@example.com 020 7908 6450 Notes to Editors Unless otherwise credited, statistics included were sourced from a survey of 1,000 UK adults, carried out by Morar in February 2016 on behalf of The Open University. About The Open University: · The Open University (OU) is the largest academic institution in the UK and a world leader in flexible distance learning. Since it began in 1969, the OU has taught more than 1.8 million students and has almost 180,000 current students, including more than 15,000 overseas. · The OU was given an overall satisfaction rating of 90% in the latest National Student Survey, making it one of only three Higher Education Institutions (HEIs) to consistently score 90% or above every year since 2007. Over 70% of students are in full-time or part-time employment, and four out of five FTSE 100 companies have sponsored staff to take OU courses. · In the latest assessment exercise for university research (Research Excellence Framework, 2014), nearly three quarters (72%) of The Open University's research was assessed as 4 or 3 star – the highest ratings available – and awarded to research that is world-leading or internationally excellent. The Open University is unique among UK universities having both an access mission and demonstrating research excellence. · The OU has a 42 year partnership with the BBC and has moved from late-night lectures in the 1970s to co-producing prime-time series such as The Hunt, Life Story, The Bottom Line, Britain's Great War, I Bought a Rainforest and Business Boomers. In 2013/14 OU co-productions were viewed by 220m people in the UK which prompted more than 600k visits the OU's free learning website, OpenLearn. (http://www.open.edu/openlearn/). · Regarded as Britain's major e-learning institution, the OU is a world leader in developing technology to increase access to education on a global scale. Its vast 'open content portfolio' includes free study units on OpenLearn, which received 5.2million unique visitors in 2012/13, and materials on iTunes U, which has recorded more than 66 million downloads. · For further information please visit: www.open.ac.uk This press release was distributed by ResponseSource Press Release Wire on behalf of The Lexis Agency in the following categories: Leisure & Hobbies, Women's Interest & Beauty, Education & Human Resources, for more information visit http://pressreleasewire.responsesource.com/about.


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The ability to image and ultimately quantitate beta-cell mass in vivo will likely have far reaching implications in the study of diabetes biology, in the monitoring of disease progression or response to treatment, as well as for drug development. Here, using animal models, we report on the synthesis, characterization of, and intravital microscopic imaging properties of a near infrared fluorescent exendin-4 analogue with specificity for the GLP-1 receptor on beta cells (E4K12-Fl). The agent demonstrated sub-nanomolar EC50 binding concentrations, with high specificity and binding could be inhibited by GLP-1R agonists. Following intravenous administration to mice, pancreatic islets were readily distinguishable from exocrine pancreas, achieving target-to-background ratios within the pancreas of 6:1, as measured by intravital microscopy. Serial imaging revealed rapid accumulation kinetics (with initial signal within the islets detectable within 3 minutes and peak fluorescence within 20 minutes of injection) making this an ideal agent for in vivo imaging.
Beta-cell mass (BCM) and the functional state of islets are critical measures when assessing the magnitude of autoimmune destruction in type 1 diabetes (T1D). Progressive loss of BCM is also believed causative in the secondary failure of many therapies in type 2 diabetes. Serum tests such as stimulated insulin or C-peptide do not reliably correlate with BCM, particularly at the extremes of BCM (1). The gold standard continues to be the laborious task of measurement at autopsy via morphometric methods. It is now believed that imaging technologies could ultimately be exploited to a) better understand the history and pathophysiology of diabetes, b) enable earlier diagnosis of T1D perhaps while in the occult phase, c) allow monitoring of therapeutic efficacy and durability (including islet transplantation), and d) facilitate the discovery of new therapies. Unfortunately, the necessary tools for reliably quantifying BCM in vivo have remained elusive.
A number of imaging agents with putative beta cell specificity have been investigated, partly based on known markers/functions of these cells (e.g., glucose transporters or sulfonylurea receptors) and partly based on profiling studies (reviewed in (2–4)). Many of the imaging studies to date have relied on isotope-labeled compounds including compounds targeting sulfonylurea receptors (5), neuroreceptors such as the pre-synaptic vesicular acetylcholine transporter (6), the vesicular monoamine transporter (VMAT2) using the neurotracer 11C-dihydroterabenazine (11C-DTBZ) (7–10), or an antibody against a pancreatic islet antigen (IC-2) (11). Unfortunately, these agents have not been proven effective for in vivo islet imaging, most likely on account of their failure to achieve the required high binding ratios necessary for macroscopic imaging or a lack of understanding of the physiology of the underlying target (5, 12). Alternatively, to image at higher spatial resolution than possible with isotopic imaging techniques, a variety of fluorescent compounds have also been suggested. Labeled glucoses (13), fluorescent streptozotocin analogs (14), and Zn-chelators (which bind zinc rich insulin vesicles) (15), antibodies (12) or activatable fluorescent substrates for dipeptidyl peptidase 4 (DPP4) (16) have all been utilized. Many of these probes have been tested primarily in insulinoma or in other cells expressing high levels of glucose transporter 2 (GLUT2), where they have shown some success in vitro but not in vivo. However, it is not until recently that intravital imaging approaches have emerged as a method to validate the behavior of labeled agents in the pancreas in vivo (17–20).
Based on the recent success of therapeutic targeting of the glucagon like peptide-1 receptor (GLP-1R) in the clinic with agonists (21, 22), we hypothesized that certain fluorescent exendin-4 analogs could be used to image beta cells in vivo, but that detailed investigations would be necessary to assure target binding of putative key compounds. In this report, we systematically investigated several analogs and characterized the most promising candidates before performing in vivo validation studies including intravital imaging. Our results demonstrated high islet specificity within the pancreas in vivo with the compound (E4K12-Fl).
All reagents were purchased from Sigma-Aldrich (St. Louis, MO) and used without further purification unless otherwise noted. Exendin-4 was obtained from Prospec (Rehovot, Israel). Near-infrared fluorochrome Vivotag-680 (VT680-NHS ester) was purchased from VisEn (Bedford, MA). LC-ESI-MS analysis and HPLC-purifications were performed on a Waters (Milford, MA) LC-MS system. For LC-ESI-MS analyses, a Waters XTerra® C18 5 μm column was used. For preparative runs, an Atlantis® Prep T3 OBD™ 5 μM column was used. MALDI-MS spectra were collected on a Voyager-DE™ Biospectrometry™ Workstation spectrometer (Applied Biosystems™, Foster City, CA).
To a solution of 10 mg (46 μmol) 11-Azido-3,6,9-trioxaundecan-1-amine in 300 μL 10X PBS, 2.5 mg (2 μmol) VivoTag 680 was added and the reaction solution stirred at room temperature in the dark for 2 hours. The crude mixture was directly subjected to HPLC-purification, yielding 1.5 mg (1.1 μmol, 56%) of the title compound as a blue solid. ESI-LC-MS m/z (%) = 1342 [M+H+]+.
To a solution of 0.8 mg (0.2 μmol) exendin-4(40-Pra) in 400 μL of a 25 mM Tris-buffered aqueous solution (pH = 7.2), 50 μL of a 50 mM solution of L-ascorbic acid in H2O and 50 μL of a 50 mM aqueous CuSO4 solution in H2O was added. 0.4 mg (0.3 μmol) of 11-Azido-3,6,9-trioxaundecan-1-amide-VT680 in 500 uL 2X PBS solution was then added and the reaction mixture was gently stirred at room temperature in the dark over night. The crude mixture was diluted with 2 mL of 1X PBS and purified using an Amicon® Ultra 3 kDa (Millipore, Carrigtwohill, Ireland) centrifugal filter, before it was subjected to HPLC purification, yielding 0.3 mg (0.05 μmol, 25%) of the title compound as a blue film. ESI-LC-MS m/z (%) = 1405 [M-4H+]4− (100); 1873 [M-3H+]3− (14).
To a solution of 250 μg (0.06 μmol) of Exendin-4 in 3.6 mL of a 25 mM Tris-buffered aqueous solution (pH = 7.2), 1 mg VivoTag 680 (0.8 μmol) was added and the reaction mixture stirred at room temperature in the dark for 3 hours. The mixture was subjected to HPLC-purification, yielding the title compound as a blue solid (100 μg, 0.02 μmol, 33%). ESI-LC-MS m/z (%) = 1769 [M-3H+]3− (100).
To a suspension of sinapinic acid (5 μL, 10 mg/mL, AcN/H2O = 1/1, 0.5 % TFA), 5 μL of protein sample (approx. 100 μM in H2O) was added and the carefully mixed solution purified via ZipTips (Millipore, Billercia, MA) prior to measurement. Each sample was also run with adrenocorticotropic hormone fragment 18–39 (ACTH fragment 18–39) as a calibration standard.
To a solution of E4K12-Fl (10.5 μL, approx. 10 μg), 15 μL NaHCO3 (50 mM) and 1.5 μL dithiothreitol (DTT; 100 mM) was added, and the mixture was incubated at 95 °C for 5 minutes. Subsequently, 3 μL of IAA (100 mM) was added and the sample was incubated at room temperature for 20 minutes, before 1 μL of activated trypsin (100 ng/μL, Pierce, Rockford, IL) was added. This was followed by an incubation at 37 °C for 3 hours. An additional 1 μL of activated trypsin (100 ng/μL) was then added and the sample was further incubated at 37 °C for 2 hours, before being purified using ZipTips (Millipore, Billerica, MA) and subjected to MALDI-MS analysis.
Mouse insulinoma-derived MIN6 cells, courtesy of Dr. Miyazaki (23), were used between passages 26 and 40 and grown in high-glucose DMEM containing 15 % (v/v) heat-inactivated FBS, 50 U/ml penicillin, and 10 μg/ml streptomycin as reported previously (24). HEK 293 cells, stably expressing human GLP-1R (HEK/hGLP-1R) (25),were grown in high glucose DMEM containing 10% (v/v) heat-inactivated FBS, 50 U/ml penicillin, 10 μg/ml streptomycin, 1 mM sodium pyruvate, and 150 μg/ml G418. NIH-3T3 fibroblasts (ATCC) were grown in high glucose DMEM containing 10% (v/v) heat-inactivated FBS, 50 U/ml penicillin, and 10 μg/ml streptomycin.
MIN6, HEK/hGLP-1R or NIH-3T3 cells, seeded into 6-well plates were washed twice with ice-cold PBS and lysed on ice with 200 μl ice-cold RIPA lysis buffer. Protein concentrations were determined using bicinchoninic acid (BCA) protein assays (Pierce, Rockford, IL). Cell lysates (50 μg) were subjected to SDS PAGE, followed by immunoblotting using a specific anti-GLP-1R antibody (Abcam), and detection with chemiluminescence (Pierce, Rockford, IL).
MIN6 (2×105 cells/well), HEK/hGLP-1R (7.5×104 cells/well) or NIH-3T3 (7.5×104 cells/well) cells were seeded into 8-well chamber slides (LabTek, Rochester, NY) and incubated for 48 hours before incubation with 100 nM of E4K12-Fl for the indicated time points at 37 °C. Cells were washed three times with PBS before subjected to imaging. MIN6, HEK/hGLP-1R and NIH 3T3 cells were imaged on an Olympus FluoView1000 confocal laser scanning microscope with an Olympus XLPan N 25x (N.A. 1.1) water immersion objective and the FluoView 1000 Vers. 2.1a program. E4K12-Fl was imaged using a 635 nm laser, dichroic mirrors DM405/488/559/635 and SDM640 and a barrier filter BA655-755.
MIN6 (1×105 cells/well), HEK/hGLP-1R (3×104 cells/well) or NIH-3T3 (3×104 cells/well) cells were seeded into 96-well plates and incubated for 48 hours before adding different concentrations of E4K12-Fl. After incubating for 1 hour at 37 °C, cells were washed three times with PBS and the fluorescence levels were measured with a SynergyMx plate reader (Biotek) with excitation at 620 nm and emission at 690 nm. Following fluorescence measurement, proteins were extracted using RIPA lysis buffers (24) and quantified using BCA (Pierce, Rockford, IL).
MIN6 (1×105 cells/well), HEK/hGLP-1R (3×104 cells/well) or NIH-3T3 (3×104 cells/well) cells were seeded into 96-well plates and incubated for 48 hours before incubation with either exendin-4 (100 nM) for 1 hour at 37 °C and subsequently E4K12-Fl (10 nM) for 1 hour at 37 °C, or just E4K12-Fl (10 nM) for 1 hour at 37 °C. Following the incubation, cells were washed three times with PBS and the fluorescence levels were measured with SynergyMx plate reader (Biotek) with excitation at 620 nm and emission at 690 nm. Following fluorescence measurement, proteins were extracted using RIPA lysis buffers (24) and quantified using BCA (Pierce, Rockford, IL).
NOD MIP-GFP mice (NOD.Cg-Tg(Ins-EGFP)Hara/QtngJ), that express the fluorescent protein EGFP within the islets under the mouse insulin 1 promoter were obtained from The Jackson Laboratory (Bar Harbor, ME). All procedures and animal protocols were approved by the subcommittee on Research Animal care at Massachusetts General Hospital.
Nondiabetic male NOD MIP-GFP mice were anesthetized with 2% isoflurane and 2 L/minute O2. The peritoneal cavity was opened with a transverse incision in the disinfected abdominal wall. The gastric-splenic ligament was dissected and the pancreas carefully exteriorized. Robust blood ow was observed in the pancreatic arteries for the duration of each experiment and pancreas perfusion was confirmed by observation of a circulating fluorescent vascular agent (Angiosense 750, VisEn, Bedford, MA). The exteriorized pancreas was completely submerged in temperature-controlled lactated Ringer's solution. Temperature near the spleen was carefully monitored using an Omega HH12A thermometer with ne wire thermocouples (Omega Engineering Inc., Stamford, CT) and kept at 37 °C. A tail vein catheter, made using a 30G × ½″ needle inserted into CLAY ADAMS® Polyethylene10 tubing, was inserted prior to surgery. Subsequently, 200 μL (4 nmol/200 μL) of E4K12-Fl and 10 minutes after the initial injection, another 200 μL (4 nmol/200 μL) of E4K12-Fl was injected via the tail vein catheter.
Images were collected with a prototypical intravital laser scanning microscope (IV100, Olympus Corporation, Tokyo, Japan) (26) using an Olympus 4x (UplanSApo N.A. 0.16), and 20x (UplanFL N.A. 0.5) objective and the Olympus FluoView FV300 version 4.3 program. Samples were excited at 488 nm with an air-cooled argon laser (Melles Griot, Carlsbad, CA) for visualization of the GFP expressing islet-cells, at 633 nm with a HeNe-R laser (Model 05LHP925, Melles Griot) for visualization of E4K12-Fl, and at 748 nm with a red diode laser (Model FV10-LD748, Olympus Corporation, Tokyo, Japan) for visualization of the blood pool agent (AngioSense-780, VisEn Medical, MA). Light was collected using custom-built dichroic mirrors SDM-560, SDM640, and SDM-750, and with emission filters BA 505-550, BA660-730, and BA 770 nm IF (Olympus Corporation, Tokyo, Japan). The E4K12-Fl signal was collected sequentially to the other channels to avoid bleed-through into the VT680 channel. A prototypical tissue stabilizer (Olympus Corporation, Tokyo, Japan) was used to reduce motion and to stabilize the focal plane. The stabilizer was attached to the objective and its z-position was finely adjusted using a micrometer screw to apply soft pressure on the tissue.
Nondiabetic male NOD MIP-GFP mice were cannulated with a tail vein catheter, made using a 30G × ½″ needle inserted into CLAY ADAMS® Polyethylene10 tubing. Subsequently, 200 μL (4 nmol/200 μL) of E4K12-Fl was injected via the tail vein catheter. Mice were sacrificed 2 hours after the initial injection, the pancreas was harvested and frozen in OCT compound (Sakura, Finetek, Torrance, CA) with isopentane on dry ice. The frozen tissues were sectioned (thickness: 6 μm) and mounted on microscope slides. Without further processing, tissue samples were excited with an EXFO X-CiteTM 120 light source (EXFO Photonic Solutions Inc. Ontario, Canada) and imaged with a Nikon Eclipse 80i microscope with either a 10x Plan Fluor N.A. 0.3 and a 20x Plan Apo 0.75 objective (Nikon corporation, USA) respectively. GFP fluorescence was observed with a Chroma HQ FITC filter cube (HQ480/40x EX, dichroic Q505LP BS, and emission filter HQ535/50m EM) and Exendin-VT680 fluorescence with a Chroma Cy5.5 filter cube (HQ650/45x EX, dichroic Q680LP BS, and emission filter HQ710/50m EM; Chroma Technology Corp., VT, USA). Images were captured with a Cascade Model 512B camera (Roper Scientific, Tucson, Arizona). Validation of fluorescence signals employed immunohistochemistry for insulin (Insulin (H-86), Santa Cruz Biotechnology, Santa Cruz, CA) using the avidin-biotin peroxidase method. The reaction was visualized with a 3-amino-9-ethyl-carbazole substrate (AEC, Sigma Chemical, Saint Louis, Missouri). Adjacent sections treated with nonimmune IgG provided controls for antibody specificity.
Near infrared fluorescent exendin-4 analogues were created via two different strategies (Fig. 1). In the first strategy, an alkyne-modified artificial amino acid (S)-2-amino-4-pentynoic acid was attached to the C-terminal end of exendin-4, yielding the peptide Exendin-4-Pra. To this peptide, an azide-modified near infrared (NIR) fluorochrome 11-azido-3,6,9-trioxaundecan-1-amide-Fl was covalently attached using standard copper catalyzed click-chemistry protocols, yielding the exendin-4-like NIR-fluorochrome, E440-Fl. 11-azido-3,6,9-trioxaundecan-1-amide-Fl was generated in good yields (56%) by conjugation of 11-azido-3,6,9-trioxaundecan-1-amine to the commercially available NIR fluorochrome VT680-NHS ester in the presence of triethylamine. The structural identity of the exendin-like NIR-fluorochrome E440-Fl, was confirmed using liquid chromatography-mass spectrometry (LC/MS) analysis, which confirmed the expected mass of 5623 g/mol (m/z (%) = 1405 [M-4H+]4 − (100); 1873 [M-3H+]3 − (14)). The second approach was based on the direct reaction of VT680-NHS ester with exendin-4, facilitated in a Tris-HCl buffered aqueous solution. Although exendin-4 offers multiple nucleophilic sites, which can all theoretically react with NHS esters at one or multiple sites, only three reaction products were detected in the crude reaction mixture via LC/MS under current reaction conditions. All of the products had the same mass of 5311 g/mol (m/z (%) =1769 [M-3H+]3− (100); Fig. 2b), which corresponded to the addition of a single fluorochrome per exendin-4. Three products were observed in ratios of 5/5/90. The major product (90 %) was isolated using preparative HPLC, yielding the exendin-4-like NIR fluorochrome E4K12-Fl (Fig. 1).
The mass of E4K12-Fl was analyzed using matrix assisted laser desorption ionization mass spectrometry (MALDI-MS), which confirmed the exendin-4-like NIR fluorochrome to be composed of exendin-4 and a single fluorochrome molecule (m/z = 5311, Fig. 2c). Structural analysis of the compound was determined by tryptic digestion of both E4K12-Fl and exendin-4. Whereas exendin-4 displayed two strongly ionizing fragments at 1278 Da (H1-K12) and 948 Da (L21-K27), tryptic digest of E4K12-Fl did not result in the formation of a 1278 Da fragment, but showed a dominant peak at 3373 Da (Fig. 2d). The fragment corresponded to amino acids H1-R20 (2251 Da) plus the mass of the fluorochrome (1123 Da). Observation of a peak at 3373 Da for E4K12-Fl but not for exendin-4 indicated that the fluorochrome was part of that fragment and that it was conjugated to amino acid K12. If the fluorochrome were not conjugated to K12, the fragment would have been digested by trypsin at the K12-position into two smaller fragments. The predominant attachment of the fluorochrome to K12 compared to other nucleophilic residues (e.g. K27 or the N-terminus) is likely explained by either sterically or electronically favored conformations of K12.
In order to determine accumulation of E4K12-Fl on GLP-1R expressing cells, we tested the compounds against human GLP1-R overexpressing HEK/hGLP-1R cells (HEK/hGLP-1R), GLP-1R naturally expressing MIN6, and against GLP-1R negative NIH 3T3 cells (Fig 3). The expression levels of GLP-1R were then analyzed by staining the respective cell lysates with an anti-GLP-1R antibody and equal cell lysate concentrations were analyzed by staining against tubulin in western blots (Fig. 3). Western blotting confirmed GLP-1R to be absent in NIH 3T3 cells and overexpressed in transformed HEK/hGLP-1R cells. Each cell line was incubated with different concentrations of E4K12-Fl for 1 hour. The cells were later washed and their fluorescence measured. The fluorescent signal was normalized to the number of cells present in each well. GLP-1R overexpressing HEK/hGLP-1R cells displayed the highest fluorescence, followed by insulinoma-derived MIN6 cells. GLP-1R negative NIH 3T3 cells did not show any discernible fluorescence (Fig. 3a). For HEK/hGLP-1R cells, a binding EC50 of 0.3 ± 0.2 nM was determined and for MIN6 cells, the observed EC50 was 0.5 ± 0.3 nM. These findings were corroborated using confocal microscopy (Fig. 3c). Blocking experiments with non-fluorescent exendin-4 were subsequently conducted. Pre-treated HEK/hGLP-1R cells were observed to have a 12-fold lower fluorescence and pre-treated MIN6 cells a 5-fold lower fluorescence. Finally, we also tested the terminally labeled E440-Fl (as in a previous study (19)) which did not show strong preferential accumulation in GLP-1R expressing cell lines.
In order to evaluate the performance of E4K12-Fl in vivo, NOD MIP-GFP mice were anesthetized and the pancreas exteriorized onto a heated chamber for real-time multi-channel confocal imaging. Microvasculature was imaged in the 750 nm channel after injection of AngioSense-780, a long circulating blood pool agent, and islets were identified in the 488 nm channel via their GFP expression. Following this, 8 nmol of E4K12-Fl in 200 μL of PBS was injected, and accumulation of the probe in the islets and surrounding tissue was monitored (Fig. 4). Intravital imaging confirmed that prior to imaging no signal corresponding to a pancreatic islet was observable in the E4K12-Fl channel (Fig. 5, 0 min). However, after 3 minutes, accumulation of E4K12-Fl could be observed in the pancreatic islets, and this fluorescence increased during the observation time of 20 minutes, after which steady state was reached (Target-to-background ratio of 6:1; Fig. 5). Ninety minutes after injection, the signal still displayed a ratio of 6:1. Following comparison of imaging results from the vasculature and the GFP-islets, it was confirmed that E4K12-Fl co-localized in the pancreatic beta cells, and did not simply extravasate from the vasculature that traverses the islets. During the time of measurement, no increase in background noise from surrounding tissue was observed. The target-to-background ratio of islets over exocrine pancreas was 6.0 ± 0.5 in steady state. To further confirm that probe accumulation occurs in pancreatic beta cells, we performed additional immuno- and fluorescence histology (Fig. 6). These studies also demonstrated exclusive accumulation of E4K12-Fl in the islets and specifically in the pancreatic beta cells expressing insulin and GLP-1R. Biodistribution studies show renal excretion of non-localized agent and intracellular degradation in endosomal compartments.
T1D is a classic example of an organ-specific autoimmune disease. It has two stages. The first is an occult phase, termed insulitis, in which a mixed population of leukocytes invades the islets of Langerhans of the pancreas. This, in turn, eventually provokes specific destruction of the insulin-producing beta cells. The second stage is the overt phase (diabetes), in which the bulk of beta cells have been destroyed, and insulin production is no longer sufficient to regulate blood-glucose levels. This eventually results in hyperglycemia. To date, a major hindrance to both research on and treatment of T1D, has been the inability to directly visualize beta cells during disease progression. Our goal has therefore been to develop robust, non-invasive methods to image BCM (both experimentally in mouse models and clinically). Thus far, we have tested most of the published fluorescent probes in vivo in MIP-GFP mice, but with little or modest success. Based on profiling studies, we also investigated a number of novel targets including carboxypeptidase E (CPE), proprotein convertase subtilisin/kexin 2 (PCSK2) or islet amyloid polypeptide (IAPP), among others. This study now reports on the first selective fluorophore-based probe for in vivo imaging of pancreatic beta cells, and we confirm its potency in biochemical assays, in in vitro assays, by histology and by in vivo imaging.
The islets of Langerhans, encompassing the endocrine pancreas, represent approximately 1–2% of the pancreatic mass. They are also scattered throughout the pancreas, which is likely the underlying reason for the previous difficulties in visualizing BCM with macroscopic imaging techniques (5). Within each islet, there are five major endocrine cell types: insulin-producing beta cells (60–80% of the islet), glucagon-producing alpha cells (15–20% of the islet), somatostatin-producing delta cells (5–10% of the islet), and pancreatic-polypeptide-producing (PP) cells (15–20% of the islet), and ghrelin-producing epsilon-cells (< 1%).
The adult mouse pancreas contains about 3,000 islets, with about 2×106 beta cells (27). The human pancreas contains about 106 islets. These pancreatic islets measure 50–300 μm in diameter and are highly vascularized, with 1–5 arterioles branching into numerous capillaries. Capillaries are fenestrated and are therefore highly permeable. It has been estimated that islets receive 10–20% of pancreatic blood flow, which is disproportionally large for an area that assumes 1 to 2% of pancreatic volume and < 0.005% of body weight. This feature, however, creates a favorable environment for the delivery of imaging agents. In our studies, we used intravital confocal microscopy to study islet microvascularity and were able to show rapid delivery of the E4K12-Fl agent to the pancreatic islets with high spatial overlap with EGFP labeled beta cells.
In the pancreas of mice, rats, and humans, the GLP-1R is highly expressed within the islets and to a lesser extent within some ducts. Within the islets the GLP-1R protein almost exclusively co-localizes with insulin and is highly restricted to pancreatic beta cells (28). This distribution makes the GLP-1R a good target for measuring BCM. Glucagon-like peptide-1 (GLP-1) is secreted by intestinal L cells as a gut hormone and its secretion is dependent on the presence of nutrients in the small intestine. The physiological functions of GLP-1 include potentiation of insulin secretion in a glucose dependent manner as well as influencing a number of gastric functions (slowing gastric emptying and inhibiting acid secretion). GLP-1 analogues are a new class of drugs for the treatment of diabetes as they have a low risk of causing hypoglycemia (29). Exenatide (Byetta), the first FDA approved GLP-1 analog, is a synthetic version of exendin-4, a hormone found in the saliva of the Gila monster. It has 53% sequence homology with GLP-1 (30). A recent crystal structure shows that exendin-4 binds to the extracellular domain of GLP-1R (30). From this crystal structure, it follows that the amino acid K12 is not involved in GLP-1R binding (Fig. 1) and explains its efficacy as an imaging agent. Conversely, other modifications with fluorochromes do alter binding.
In this study, we focused on the exendin-4 analog E4K12-Fl. There are, however, several other pharmaceutical analogues of GLP-1 that are currently in development or in clinical use, including liraglutide, albiglutide and taspoglutide, each of which have variable substitutions and modifications that alter their pharmacokinetics (21). It is entirely possible that we will be able to further optimize probe development by using some of these newer GLP-1 analogues as platforms. Another potential avenue for probe development would be to use the peptide as a targeting ligand for the delivery of multimodal, nanoparticle based constructs for imaging (31) and or therapeutics (32). We believe that the featured E4K12-Fl, and possibly similar derivatives, will prove useful agents for the selective visualization of beta cells. Finally, it appears reasonable that more immediately one should be able to use the described agent in the imaging of often difficult to localize insulinomas, possibly via positron emission tomography (PET) tracers, via magnetic resonance imaging (MRI) with agents based on this peptide, or by intraoperative imaging of fluorescent compounds as described.
We would especially like to thank Christophe Benoist and Diane Mathis for many helpful discussions. We acknowledge the help of Yoshiko Iwamoto for histology and Rostic Gorbatov for surgery. The work was supported in part by P01 AI54904 (DM, RK, RW), RO1 67536 (RK), U24 CA092782 (RW) and a grant from the Deutsche Akademie der Naturforscher Leopolina (German Academy of Sciences Leopoldina) to TR.
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Customers can purchase remarkable range of Chinese cooking platform, which is manufactured and supplied by our organization. Our offered cooking platforms are made in strict adherence with the international standards that also go through a quality check after these are manufactured. Some of the features of these Chinese cooking platforms are durability, high efficiency, rigid construction and longer service life. These are widely demanded by various food joints, Chinese restaurants and local fast food centers.
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and while i understand and appropriate the desire and need to push BB products, i really wish they would have gone into more details on the support for IOS and Android. i think it is a smart move, to breath more life into the RIM servers, but give me more.
2 Replies
· · ·
Dec 14, 2011 at 9:56 UTC
Got a link for the vid?
· · ·
Dec 15, 2011 at 7:54 UTC
link about half way down
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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Register now to gain access to all of our features. Once registered and logged in, you will be able to contribute to this site by submitting your own content or replying to existing content. You'll be able to customize your profile, receive reputation points as a reward for submitting content, while also communicating with other members via your own private inbox, plus much more! This message will be removed once you have signed in.
Ayreon's music is mostly heavy metal and progressive rock but combines them with genres like folk, classical and electronica. The majority of Ayreon's albums are dubbed "rock operas" because the albums contain complex storylines featuring a host of characters, usually with each one being represented by a unique vocalist.
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text: Seller: long-ago-far-away (3,203) 100%, Location: Armadale, VIC, Ships to: Worldwide, Item: 221838364003 Title: Indus Valley Clay Bowl With Pipal Leaf Design Item Code: X5834 Description: Indus Valley Merghar clay bowl with pipal leaf design. Origin: Circa 3rd millennium B.C. Indus Valley Dimensions: Height: 8.5 cm / Diameter: 16.0 cm Condition: Intact. Overseas airmail shipping is USD 30. Within Australia USD 15 We are happy to combine multiple purchases to reduce shipping costs. Please visit our About Me page for a link to our website We prefer payment via PAYPAL. We also accept major credit cards. Australian buyers may pay by direct deposit. We have nearly 25 years of experience in dealing with Ancient and Tribal art. You may purchase with full confidence. B.C. Galleries Ancient & Tribal Art 1069 High Street Armadale Victoria 3143 Australia Tel/Fax +61 3 9804 3353


text:
It's going to be an absurdly busy summer of original programming on ABC. I mean, you may not be excited about any of that original programming, but it's sure going to be filling a lot of primetime real estate.
On Monday, ABC announced a summer 2014 schedule that includes four new shows, plus eight returning series. There are scripted dramas, competitive reality shows, docudramas, things from Canada and more.
ABC's summer programming will already have started on April 24, when "Black Box" takes over the Thursday 10 p.m. slot currently occupied by the truncated third season of "Scandal."
Two other summer offerings will get a slight head-start, premiering during the last week of the official Nielsen 2013-2014.
"The Bachelorette" will premiere on Monday, May 19 from 9:30-11 p.m. after the first part of the "Dancing with the Stars" finale. "The Bachelorette" will have its regular 8 p.m. to 10 p.m. time period premiere on May 26 and on Monday, June 2 the second season of "Mistresses" will take over the Monday 10 p.m. hour. [The six-part "Bachelor" spinoff "Bachelor in Paradise" — think "Bachelor Pad" only in a different location — will premiere on Monday, August 4 and will air in the 8 to 10 spot.]
Also sneaking out early is the drama "Motive," a Canadian hit and a semi-solid performer for ABC last summer. "Motive" will premiere on Wednesday, May 21 at 10 p.m. ABC won't be airing anything else on Wednesdays this summer.
Actually, let's just go night-by-night, eh? [I'll do a list below.]
We've got Mondays covered, so original summer Tuesday programming will begin on May 27 with "Extreme Weight Loss" airing from 8 to 10. "NY Med" will return on June 24 in the 10 p.m. hour.
Thursdays will be a big more active. "Black Box" will keep the night warm through June 19 when the odd fantasy-reality hybrid "The Quest," which finds contestants completing quests in a mythical real with the use of animatronics, prosthetics and real-time motion capture, takes over the 8 p.m. hour. "Rookie Blue" will return for yet another season of Canadian-accented police procedure and soapy romance on Thursday, July 17. Then, on July 24, ABC will premiere the period drama "The Astronaut Wives Club" from executive producers Stephanie Savage and Josh Schwartz, featuring an ensemble led by JoAnna Garcia Swisher, Odette Annable, Zoe Boyle and Desmond Harrington.
Sundays will also be busy with "Wipeout" getting a two-hour premiere on June 22 from 7 to 9, leading into the singing competition "Rising Star," which will feature some live-voting component and presumably largely disenfranchise West Coast viewers. "Wipeout" will take its regular 8 p.m. hour the following week, while "Rising Star" will air from 9 to 11.
Oh and "Bet On Your Baby" will air on Saturdays at 8 p.m. starting on May 31.
So that's four new shows — "The Quest" (June 19) "Rising Star" (June 22), "The Astronauts Wives Club" (July 24) and "Bachelor in Paradise" (August 4) — plus the returns of "The Bachelorette" (May 19), "Motive" (May 21), "Extreme Weight Loss" (May 27), "Bet On Your Baby" (May 31), "Mistresses" (June 2), "Wipeout" (June 22), "NY Med" (June 24) and "Rookie Blue" (July 17).
Anything there exciting you? Seriously?!?


text:
Baby Cheetah Facts
Cheetah is a Python-powered template engine and code generator. Cheetah project can be used combined with other tools and frameworks or as a standalone application. Web development is its principle use, but Cheetah is very flexible and is also being used to generate C++ game code, Java, sql,...
Platforms: *nix
|License: Freeware
|Size: 163.84 KB
|Download (116): Cheetah 2.0 Download
Each curious key press entertains your baby with delightful animations and cute loveable animated characters. Babies are comfortably introduced to the computer and learn cause-and-effect relationships.
We encourage you to further tailor your baby's learning experience by discussing the animals...
Platforms: Mac
|License: Freeware
|Size: 43.4 MB
|Download (52): Giggles Computer Funtime For Baby: My Animal Friends for Mac OS Download
Baby Names helps you print high quality personalised scrolls, greeting cards, fridge magnets, keyrings, mugs, mouse mats, coasters, desk clocks and pens based upon any baby name.
Choose from many different designs to produce the perfect gift for friends & family. You can choose the occasion and...
Platforms: Windows, 2K, XP, 2003, Windows Vista, 7, 7x64
|License: Freeware
|Download (34): Baby Names 2011 Download
Every parent or babysitter knows that it's hard to surf the web or simply operate a computer while having a baby or a small kid in your lap. They need to be kept constantly occupied and are also very curious. Most often then not they end up pressing the wrong button on the keyboard and close or...
Platforms: Windows
|License: Freeware
|Size: 1.82 MB
|Download (33): Baby Computer Piano Download
A program that allows you to get daily random facts right onto your desktop without any installation needed. Read over thousands of funny interesting facts that you can use to amaze and astound your friends and family. The facts list is constantly updated. Internet connection required.
Platforms: Windows
|License: Freeware
|Size: 604 KB
|Download (70): Debrain facts generator Download
Mommy & Baby Animals Screen Saver featuring 20 adorable images of wild animal babies with their mothers!
Platforms: Windows
|License: Freeware
|Size: 9.9 MB
|Download (30): Mommy & Baby Animals Screen Saver Download
Enjoy this screensaver featuring a collection of Baby Seal photos. Bring your desktop to life with this free Baby Seal screensaver.
Platforms: Windows
|License: Freeware
|Size: 653 KB
|Download (28): Baby Seal Screensaver Download
Baby Tips is a colorful presentation which every slide in it shows a tip for one of the 12 months in your child first year.
Platforms: iPhone
|License: Freeware
|Size: 1024 B
|Download (68): Baby Tips for iPhone Download
MB Baby Name Numerology does baby name numerology analysis and gives you an insight into the characteristic features and personality traits of your child. Using this free tool for baby name numerology interpretation, you also get the scope to choose a name for your child that could influence his...
Platforms: Windows
|License: Freeware
|Size: 730 KB
|Download (59): MB Baby Name Numerology Download
The Baby Tooth chart is a PHP script which graphically shows how a baby's primary teeth have erupted - Eventually it'll become a POST/PHP-Nuke module. It uses only PHP and static images (i.e. no PHP graphics or FLASH, so no extra libaries needed).
Platforms: Windows, Mac, *nix, PHP, BSD Solaris
|License: Freeware
|Download (31): Baby Tooth chart Download
This application will generate and save html/css based Supplement facts panels on your system desktop. While allowing you to edit the code during creation. These files are ready for upload to servers, websites, or may even just me sent by E-mail as attachments. If you like and use or have any...
Platforms: Mac
|License: Freeware
|Size: 51.2 KB
|Download (16): Supplement Facts Generator Download
Cheetah is an open source template engine and code generation tool, written in Python. It can be used standalone or combined with other tools and frameworks. Web development is its principle use, but Cheetah is very flexible and is also being used to generate C++ game code, Java, sql, form emails...
Platforms: *nix
|License: Freeware
|Size: 184.32 KB
|Download (16): Cheetah for Linux Download
Interesting Facts Screen Saver is a great way to power up your laptop or PC with fun and interesting facts. This screen saver features a nice collection of interesting facts with different categories. For example, you can find facts of animals, New York, water, money, war and many more. This...
Platforms: Windows, Windows 7
|License: Freeware
|Size: 437.71 KB
|Download (88): Interesting Facts Screen Saver Download
iList Apps Baby Registry Lite helps take the stress out of planning your baby shower. Instead of creating a separate baby registry at every store in town, this app lets you quickly create just one registry. Congratulations! You just found out that you're having a baby and you couldn't be...
Platforms: iOS
|License: Freeware
|Size: 2.3 MB
|Download (7): Baby Registry Lite Download
Download iPenguin if you love penguins. Features: - Interesting facts about penguins. - A cute, cuddly, baby penguin. Requirements: Windows Phone 8.1, Windows Phone 8, Windows Phone 7.5
Platforms: Windows Phone
|License: Freeware
|Size: 1024 KB
|Download (7): iPenguin Download
An elegant, free, Bible tool for Baby Boomers who want a few big buttons, big text and easy to use features without clutter. Features include: Easy navigation, a search box that works just like Google, one click access to email, printing and a clean, uncluttered user experience. No previous Bible...
Platforms: Windows
|License: Freeware
|Size: 5.72 MB
|Download (173): Free Baby Boomer Bible Browser Download
The free Basic Facts Worksheet Factory can generate worksheets that will provide students with the practice they need to become proficient in the basic math operations. You can easily create unlimited customized worksheets for the practice of arithmetic facts in addition, subtraction,...
Platforms: Windows
|License: Freeware
|Size: 6.27 MB
|Download (202): Basic Facts Worksheet Factory Download
This software lets you create a multimedia baby album quickly and easily by adding pictures, drawings, video clips and even audio clips directly into your pages. Discover over 40 pages organized in chapters to fill in as your child grows. In a few clicks of your mouse, you can create your...
Platforms: Windows
|License: Freeware
|Size: 12.9 MB
|Download (150): Baby Album - Basic Edition Download
Kwik Facts is a US oriented desktop reference presented in the form of a Digital Web Book. The 2007/2008 edition of Kwik Facts puts the following information right on your desktop: Monthly Calendars; Holidays & Significant Dates; Phases of the Moon [Full, New, First and Last Quarters]. Metric...
Platforms: Windows
|License: Freeware
|Size: 297 KB
|Download (75): Kwik Facts Download
Adam - the baby is a labyrinth-game with 50 changeable mazes. You can change the mazes look and balloons placement etc. High-Score-list. DirectX-sound.
Platforms: Windows
|License: Freeware
|Size: 1.9 MB
|Download (82): Adam - The Baby Download


text: Recently there was a very long week break because it was Eid Mubarak where the Muslims celebrate the end of Ramadhan. I took this opportunity to photograph my nieces who happen to be on a weeks holiday from school. My first modeling shoot. Not too great but it was a fun day out. Thankfully it rained the night before and it was a cool morning. Unfortunately lighting became an issue. Good and bad. Here are some of the results.


text:
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text:
|You took my daughter away so how about I make yours disappear?
"Van Pelt Reunion"
Season 4, Episode 12
|Air Date
July 21, 2015
|Production code
Catharina Ledeboer & Jeff Sayers
Clayton Boen
|Episode Guide
Emma misses her mother and becomes consumed with finding out more about her; an unwelcome guest appears at the Van Pelt reunion.
- Paola Andino as Emma Alonso
- Paris Smith as Maddie Van Pelt
- Daniela Nieves as Andi Cruz
- Nick Merico as Daniel Miller
- Denisea Wilson as Katie Rice
- Autumn Wendel as Sophie Johnson
- Tyler Alvarez as Diego Rueda
- Rahart Adams as Jax Novoa
- Zoey Burger as Gigi Rueda
- Katie Barberi as Ursula Van Pelt
- René Lavan as Francisco Alonso
- Elizabeth Elias as Mia Black
- Julia Antonelli as Jessie Novoa
- Richard Lawrence O'Bryan as Jake Novoa
- Betty Monroe as Liana Woods-Novoa
- An intruder begins to enter Emma's house, until it is revealed to be Jax.
- Jax and Liana reunite with each other once again.
- Liana explains why she left Jax when he was younger.
- Liana left when Jax was seven.
- Jake wanted to make Jessie the Chosen One, while Liana wanted to have a happy family.
- Liana wanted to bring Jax with her and Jessie, but he protected himself when he was younger.
- Upon seeing Jax and Liana together, it makes Emma miss her mother.
- Maddie and Diego miss each other terribly.
- Andi wants to begin her guardian training.
- Emma is distracted of missing her mother and doesn't listen to Andi's desire to start her guardian training.
- Emma refuses to let Andi begin her training.
- Emma confronts her father regarding photos of her mother.
- Sophie and Katie attempts to help Maddie move on from Diego.
- Maddie decides to win Diego back.
- Fransico discovers Emma to be missing.
- Maddie and Diego get back together.
- Emma talks to Jax about missing her mother.
- Andi and Daniel talk about their problems.
- Daniel misses Mia, Billy and the Sanctuary.
- Daniel asks Andi to help him meet the Council to which she agrees to help.
- Maddie and Diego arrive at the Van Pelt reunion together.
- Maddie stands by Diego.
- Ursula allows Diego to stay at the reunion.
- Fransico shows Emma a scrapebook of her mother.
- Jessie learns that Jax went to see their mother.
- Ursula becomes the new leader of the Van Pelt family.
- Jake Novoa arrives at the Van Pelt Reunion.
- Diego repeals Jake's magic, turning him into a hamster.
- Diego saves Maddie from Jake Novoa and is finally accepted by Ursula and her family.
- Emma gains an idea to go back in time to save her mother.
The image gallery for Van Pelt Reunion may be viewed here.
|Emma (after seeing it was Jax who came through the door): Jax?
|Jax (confused): Em?
|Liana: Jax.
|Jax (suprised): Mum!
|Andi: Hey! Feeling kind of left out over here.
|Jax: Mum, what are you-woah! (falls to the ground) Um, a little help?!
|Ursula (knocking on the door): Maddie-winky! How's it going in there? Did you find something that screams "reunion fab"? (walks in and sees her in bed eating ice cream) What, uh, what is that?
|Maddie: Oh relax. It's Italian.
|Ursula: Oh, is this some new trend that I haven't heard about? *gasps* Ooh! If it is, could you get me one in fuchsia?
|Maddie: It's my 'lay in bed and wallow in misery' outfit.
|Ursula: Oh. Well in that case, it's nothing that a quick makeover won't fix. *claps* Girls! (Katie and Sophie walk in with bags of supplies)
|Maddie: I'm not going to the reunion. I'm not sure I'll ever get out of this bed, or out of this onesie, *she rubs the onesie* EVER AGAIN!
|Sophie: Ooh! Does that mean your shoe collection will be up for grabs?
|Maddie: Yeah yeah, it's all yours. *take another scoop of ice cream*
|Emma: I'm jealous of you Jax.
|Jax (confused): Wait, what?
|Emma: Wait, let me try that again. I'm happy for you, but, upset for myself. It's hard to describe. I was watching you and your mom today and I can't help thinking I wish I has a chance at that.
|Jax: Wow. I'm sorry Emma. I wish I had the chance to give you that; I would in a second. But look at the bright side of things, you have an amazing parent who really, really cares about you. (They smile at each other)
|Daniel (to Andi, sitting down at her table at the Seven): Thanks for meeting me here. I really need your help and it doesn't seem like anybody else wants to listen.
|Andi: Know how that feels. I mean, I tried to talk to Emma to get this whole guardian thing going, and she completely blew me off.
|Daniel (venting): It's just that I don't understand why you guys brought me back here.
|Andi (also venting, not paying attention to what Daniel's saying): And then she tried to talk me into this whole "not the time" bit, and I'm just like, 'Uh, no time like the present time!' Am I right?
|Daniel (still venting, not paying attention to what Andi's saying): I was happy where I was. Now Billy is gone, Mia hates me, and some other family runs the sanctuary. *pause*
|Andi: Good talk.
|Daniel: Totally.


text:
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electrical safety product Events
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Related terms for "electrical safety product ": safety product events , electrical safety events
The Battery Show Exhibition & Conference is a showcase of advanced battery technology for electric & hybrid vehicles, utility & renewable energy support, portable electronics, medical technology, military and ...
Thailand LAB INTERNATIONAL is organized by VNU Exhibitions Asia Pacific Co., Ltd. a joint venture between Jaarbeurs from the Netherlands and TCC Exhibition and Convention Center from Thailand. VNU Exhibitions Asia Pacific develops, with industry partners and exhibition organizers from around the world, executes and internationalizes trade show and conferences from animal production and ...
The International Construction and Utility Equipment Exposition (ICUEE), also known as The Demo Expo, is the premier event for utility professionals and construction contractors to gain comprehensive insight into the latest technologies, innovations, insights, and trends affecting their ...
The 18th edition of SICUREZZA, the two-yearly international exhibition on Security & Fire Prevention, has closed its doors, leaving both exhibitors and visitors well satisfied. The 2015 edition was held in Fiera Milano from 3 to 5 November and was the first in the odd-numbered years. A range of products to keep you on the forefront of technological development, with integration as its ...
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text:
Welcome to Ms. Campbell's & Ms. Stadlhofer's PYP 3!!!
Unit 1
Central Idea:
Making balanced choices about daily routines enables us to have a healthy lifestyle
Lines of Inquiry:
• Nutritious food
• Benefits of exercise
• Emotional wellbeing
Central Idea:
The influence of Romans and Ancient Egyptians can still be seen today
. Lines of Inquiry:
• Life in these ancient civilizations
• Connections to modern life
Central Idea:
Our need to share our feelings and ideas drives us to develop effective ways to communicate
Lines of Inquiry:
• Different forms of communication
• History of communication
• How people communicate in different or difficult situations


text:
Enjoy unique and entertaining learning experiences not included in a typical visit to the museum! Program included with regular admission fees. Special group admission rate. Purchase 2 adult tickets and receive 3 youth tickets free.
Grab your sled and cruise down the snow covered slopes of Pennsylvania's legendary oil boomtown! It's the perfect way to shake off your winter blues. Participants will enjoy hours of fun in the snow, hot cocoa, snacks, history and more. Donations welcome. Pithole Visitor Center 14118 Pithole Road, Pleasantville, PA 16341.
Spend six consecutive Thursday evenings at Drake Well Museum and Park enjoying music, lectures, workshops or historic demonstrations related to the people, places and events of petroleum history. Special admission fees apply. Doors open at 6:00 p.m. Program begins at 6:30 p.m.
Celebrate Pennsylvania's Birthday! A statewide celebration, Charter Day commemorates the founding of Pennsylvania through the 1681 charter that King Charles II of Great Britain granted to William Penn. Free Admission.
Enjoy a 5 or 13 mile race beginning at Drake Well Museum and Park and traveling through scenic Oil Creek State Park. All proceeds will benefit the Mobile Energy Education Training Unit (MEET-U), an energy education outreach program managed by the Friends of Drake Well, Inc. in partnership with the Pennsylvania Historical and Museum Commission. Registration fees apply.
Join us as we celebrate the opening of the Pithole visitation season. Visitors will enjoy food, music, guided tours and a Vintage Baseball game, featuring the Addison Mountain Stars vs. the Somerset Frosty Sons of Thunder. Come explore Pennsylvania's legendary oil boomtown from 10:00 a.m. until 4:00 p.m.! Special admission fees apply.
Explore Pithole, Pennsylvania's legendary oil boomtown! The Pithole Visitors Center will be open from 10:00 a.m. until 4:00 p.m. Enjoy an orientation film, exhibits and a tour of the grounds. Admission fees apply.
Visit historic sites in the Oil Region National Heritage Area and support Drake Well Museum and Park's educational mission! All proceeds will benefit the Mobile Energy Education Training Unit (MEET-U), an energy education outreach program managed by the Friends of Drake Well, Inc. in partnership with the Pennsylvania Historical and Museum Commission. Registration fees apply.
This unique Boston Marathon qualifying event starts and finishes at Drake Well Museum and Park, traveling along the historic streets of Titusville and utilizing the bicycle trail in Oil Creek State Park. All proceeds benefit educational, interpretive and community programming at Drake Well Museum and Park. Registration fees apply.
Celebrate the birth of the modern petroleum industry! Spend the afternoon uncovering the origins of oil. Work with a paleontologist to discover fossils, hammer and identify rocks with geologists, come face-to-face with dinosaurs, traverse a rock climbing wall and much more! Noon to 4:00 p.m. Special admission fees apply.
Drake Well Museum and Park will host the Annual Fall Gas Up, sponsored by the Pioneer Steam and Gas Engine Society, from 9:00 a.m. to 3:00 p.m. Displays will include antique engines, farm and oil field equipment, cars, trucks and models. Regular admission fees apply.
Join us as Pithole serves as the backdrop for an evening experience that brings oil history to life. Visitors will meet historical figures during a nighttime tour of the legendary oil boomtown, hear historical music, and enjoy the splendors of fall with seasonal refreshments and activities. Tours begin at 6:30 p.m. with the last tour departing at 9:30 p.m. Special admission fees apply.


text:
Area 42 is a top secret US Government base. It has a nuclear test site, an airfield and a Majestic base. It is an obvious parody of Area 51. In Area 42, Crypto 137 sees his past self, Cryptosporidium 136, dead. Crypto then swears revenge upon his "brother's" passing.
There is no fence around the border to the base. Instead, several sign posts state that "photography is prohibited" and "RESTRICTED AREA". In real life, Area 51 has a similar border set up. The border is marked by orange posts which are hard to see during the day, causing many to accidentally trespass on the base. The game shows the border being patrolled by the Army, while Area 51's boarders are protected by a network of ground sensors, security cameras and a private security company.
The area around the base is primarily desert. Several hills surround the facilities.
Area 42 is surrounded by mountain ranges and ridges. One of these ridges, Freedom Ridge, allowed curious tourists to see (and photograph) the entire base without fear of being caught trespassing. The exclusion zone around the Area 51 now includes Freedom Ridge. Similar hills in the game fall outside the gameplay area, although it is possible that you could have good views of the base from some of them. The newspaper that reports on events going on in Area 42 is "The Star Spangled Banner".
The base is operated by Armquist for Military testing.
Places of InterestEdit
Majestic Base - The Majestic base was at the edge of Area 42, mainly focusing on turning humans into mutants and producing radioactive cows. The base was surrounded by telsa coils. Crypto destroyed this with his Saucer.
Military Base - The Military base consisted of Soldiers, Army and Air Force Generals, and sometimes Scientists. The base is surrounded by sentry turrets and most of the base consists of Barracks and an HQ.
Nuclear Testing Site - The nuclear testing site is a small area, consisting of only 1 building. Only a small amount of Scientists and Soldiers are placed here. There are large holes in the ground due to the nuclear tests, and also a shooting range is present.
Airfield- The Airfield is adjacent to the Military Base. Three hangars are present, and one of them contains the foot of the Robo-Prez. There were a few F-86 Fighter Jets, a B-29 Superfortress and a helicopter also here, but were all destroyed when the nuke detonated.
- Area 42 is based on Area 51, as well as the running gag of 42 from The Hitchhiker's Guide to the Galaxy.
- After the mission Duck and Cover (the final mission in Area 42), Robo-Prez's foot can be seen in a hangar.
- Soldiers can be seen shooting cows on a road.
- Area 42 is the only area to predominantly feature hostiles instead of civilians.


text:
Good Afternoon My Lovely Crafting Friends
Hope you are well, I know some of you are struggling sending hugs.
This is my DT card for The Holly and Ivy Christmas Challenge
the theme this fortnight is as always Anything Christmas Goes
Onto my Card
White Card
Pion Papers
Marianne Designs Tree Die
Marianne Design No. LRO376 Die
Marianne Design LR0392
Ribbon Bow
Heart Shaped Button
Lace with Pearls
Liquid Pearls
Image from Magic Moments
I would like to enter this card into the following challenge
1. Vintage and More Challenge - On Facebook
2. Hanna and Friends Challenge # 280 - Ribbon
3. Through the Purple Haze Challenge # 131 - Vintage
4. Alphabet Challenge - L is for Lovely Lace
5. Really Reasonable Ribbon Challenge # 170 - Anything Goes with Ribbon
6. CHNC Challenge # 347 - Make it Girly
7. Craft-Dee Bowz Monthly Challenge - Anything Goes with a Handmade Bow
8. Dies R Us Challenge 79 - Anything Goes
9. Classic Design Team Open Challenge # 16 - Anything Goes
10. Kerst/Christmas - On Facebook


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REVIEW SNAPSHOT®by PowerReviews
Reviewed by 1 customer
Comments about Christian Siriano for Payless Women's Stripe Selina Tote:
The striking black and white graphic stripes on the front of this bag called out to me through the store window. The design is dramatic, stylish and classic. it is the perfect bag to take me through summer and all year round for work and play. It goes with everything. It is very well made. The faux leather is thick and sturdy and the bag can hold a lot. There is a zippered compartment and a few pockets to keep me organized. I am a big fan of Christian Siriano's designs so I am happy that he has created such an affordable line through Payless. I have received many compliments on the bag in the last few weeks. Great purchase!
- Comfort:
- Very Comfortable