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So many good truths were shared and I fear the below won't even do it justice. But I thought I'd share, to process the "big things" further, and in hopes that you might find an encouraging along the way.
It's a matter of the mind.
I'm a long-time Christian conference attendee. I've been going to conferences since I was in Jr. High and most of them end with a high, high, HIGH emotional high – the last night's talk a capstone to make you sob and recommit something – or your entire life – to Jesus. And this is not always a bad thing, but I've come to realize because it's so focused on the emotions of the heart, it's often short-lived. I've always walked away from a conference "on fire for Jesus" (or something like that), but fairly quickly hit a valley when I'm no longer sleeping next to my best friends in a hotel.
At TGCW, there was never a "moment" or a major sob-fest where I could barely breathe. Instead, I came away with my head throbbing over all the new truths I encountered and ways I wanted to change and develop my thinking. Jen Wilkin talked about how the Christian life begins with the mind, and the importance of Biblical literacy in shaping our thoughts: "Right thinking starts with right desires, which creates right actions." She charged us to "prepare our minds for action" – to battle for holiness and against passions. And the only place I'm going to get right thinking is from reading and engaging in God's word.
Often I feel incredibly overwhelmed by all that I don't know. And at TCGW, it was pretty easy to feel small and inferior when there were Christian heavy-hitters at every turn. As a mom to young children, I often feel like I don't have time to apply myself to God's word the way D.A. Carson or John Piper does, so how will I ever grow in my knowledge of God? But God's not asking me to be Piper or Carson or Wilkin. He knows exactly where I'm at and the season I'm in. God doesn't listen to Jen Wilkin's prayers more than he listens to mine. All he asks is for me to spend time with him, learning about who I am because of who he is. He grants wisdom – "right thinking" to those who ask for it. And so in this season of life, I'm learning not to live emotional high to emotional high, but instead to rest in the steady, plodding, faithful faith I've known since I was a child. To consistently study God's word in the time I have. To find something to study, and learn it. To pick one thing and apply it. To believe in the transforming power of God's word in my mind to transform my desires, to transform my actions.
Sometimes, the right answer is to choose suffering.
I plan to write about this more, but the as the adoption process progresses, I confess that I've continued to go back and forth on whether this is the right decision for our family. I know, I KNOW, deep down that God is calling us to it, but certain things have come up since we started the process that have made me question if the timing is right, and Satan continues to bring lies to mind about choosing something that will make our life "harder than it needs to be."
While much of the conference focused on suffering, Don Carson said something that really stuck with me. "Sometimes choosing the right thing – the hard way – is sometimes choosing suffering." He went on to say that believers should have a place in their life where their life costs something. "You should choose something, not because you want it, but because you should – because you want to be identified further with Christ." I have no rose-colored glasses when it comes to adoption. I know enough friends that have adopted or been adopted themselves to know that my adopted children will bring with them deep, deep wounds of suffering. But I also know enough about God to know that he can redeem all things. That he makes all things new and that his grace will be sufficient for me and my entire family, no matter what we face. It was the perfect truth I needed to shut out the lies of the devil and walk fearlessly along the path to our children. I know adoption is what God has asked of our family – he made it undeniably clear last Christmas – and now instead of wanting to withdraw every time a bump in the road comes up, I will lean into it, knowing I am identifying more and more with my Savior, every time I face hardship and I can rejoice, knowing I am counted worthy of suffering for his name.
"It's family business."
There was a powerful panel one morning on the persecuted church and K.A. Ellis said something that struck a chord with me. "Advocating for the persecuted church is not advocacy, it's family business." Growing up in a family business, this hit home. I started working for my dad as soon as his business started when I was in grade school. Sweeping and moping floors, painting trim and walls, filing paperwork that I didn't even understand how to read. It was an expectation that everyone always pull together and get things done. We always had each other's backs, protected each other and provided for each other - no matter the cost. In a family business, everyone rises together, and everyone falls together. This thinking challenged me to see my Christian brothers and sisters in a new light - we rise and fall together, we support each other, we depend on each other. Even outside of the persecuted church, in my local church, how often do I really have this thinking? One day, my blood family will fade away and I will worship before the King only with an eternal family. If I took the attitude I have for my nuclear family and applied it to the rest of the church, I think I'd be a whole lot more invested. I'd serve more. I'd give more. I'd pray more.
A fearless life because of hope.
Ten minutes of watching the news today and I can't help but be filled with sadness, anger and a whole lot of fear. And it's becoming increasingly clear that as a Christian, I am walking the uncommon, unpopular path – one that often feels lonely, scary and disliked. But a major takeaway from the conference was that I don't have to live as a fearful woman, one that is scared of the coming elections, the shifting cultural values, or the future society my children will grow up in. I live differently from the rest of the world, with an extraordinary courage, a rare fearlessness, that can only be explained by hope of something beyond this world, hope of something more than what I see around me today.
Mary Wilson reminded me that my story is not depended on what happens on the news, in the world. I may be a United States citizen on paper, but ultimately, eternally, my citizenship is in Heaven. My story is the Kingdom story, a redeemed daughter of the King's story! John Piper charged me to live in an odd, peculiar, unpopular way, because I have hope in a reward beyond this life. As a mom, I almost feel like if I'm not worried for my kids and their future, I'm not a good mom. But as a woman of God - I can laugh at the future! I have nothing to fear for me or my kids, because I know ultimately God is in charge. He will provide, protect and care for me, my husband and my babies! No matter what happens to me, no matter what suffering, pain and adversity waits, I don't have to fear! One day I will be with Christ and enjoy him forever – my glory won't happen in this life because I don't need it now – I will be exalted for eternity with Christ!
I feel like much of my motherhood has been fighting the snares of the devil telling me that I'm not good enough, I'm not doing it right, I'm not thinking ahead enough or training my children well. I've always known that I "shouldn't" fear or worry about these things, but throughout the weekend, the reasoning was solidified for me. It gives me so much freedom to mother with confidence in the gospel hope of the future coming of Christ. To know that I will not receive any reward now – and that's okay! My future reward is so much better, so much sweeter and more wonderful – IT IS WORTH THE WAIT. Oh, how I wish I help each of you mothers out there feel what I feel now! It's empowering to know that life doesn't depend on me. I can live like the great women of the Bible, having faith because of what God has done in the past, and hope for what I know he will do in the future.
And that was trying to keep it concise! There is so much more I could share, but I know this is getting long, and my coffee's getting cold. I've heard TGC is planning on putting all the talks online for free, so if you weren't at the conference, I highly encourage you to take time to listen to some of the sessions while you go about regular life. This was my first year at the conference, but it definitely won't be my last. Anyone else go? I'd love to hear about what you took away!


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Arts, Performing Arts, Society & Culture, Personal JournalsFollow
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Dramatic tales, killer beats and the edgiest new talent in storytelling come together for a weekly radio hour hosted by Glynn Washington.
July 1st, 2016
Episode 577 of 809 episodes
In 2004, aid worker Nahoko Takato was taken hostage in Iraq. When she finally returns back to Japan, she was not prepared for her homecoming. Check out her work here Producer and Sound Design: Davey Kim
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Welcome to the Brain Training Podcast, the daily audio workout for your head. In this podcast we have two games for you, each with three rounds which get progressively harder. To enjoy the full experience, relax, and avoid distractions whilst you listen.

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Freshers, IT jobs in Technopark, IT park, InfoPark and walk-in jobs
Mega ASP.NET Developer Walk in drive on 1st June 2013 at Kochi
Role: Senior Software Developer /Systems Analyst/ Senior Systems Analyst
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Experience: 2 – 10 Years
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UST Global Ground Floor, Leela Building Infopark, Kakanad Kochi-682030
WALK IN DATE: - Saturday 1st June 2013
WALK IN TIME: - Click here


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It's Pretty Unlikely That Most Undocumented Minors Will Be Granted Asylum
"Winning an asylum case when one is in detention … it's incredibly difficult." White House spokesman told reporters Monday deportations are likely for most.
WASHINGTON — Led by Sen. Robert Menendez, Democratic lawmakers have been pushing for asylum to be open to the thousands of undocumented minors who have crossed the border in recent months.
"Some of the children will qualify for protection under asylum, trafficking and other laws, while other children will not," Menendez said last month. "All of these families and children deserve to have these cases heard."
But the process for gaining asylum status in the United States is very difficult — especially in the case of minors.
Although thousands of the undocumented immigrant minors could meet the criteria for asylum in the United States, federal law also requires that minors be reunified with their parents in all cases except in situations in which reunification would be against their best interests.
"The law itself, the policy, is to reunify these children with their parents. I think that at some point, most of them are going to be going home," said David Leopold, an immigration attorney and former president of the American Immigration Lawyers Association.
"This isn't going to be one blanket grant of asylum," Leopold said.
Nearly 50,000 undocumented immigrants, many of them children, have crossed the border in recent months — a complex crisis that has overburdened U.S. detention facilities.
Republicans have argued the undocumented immigrants need to be deported with expediency. Democrats have argued many of the undocumented immigrants are "refugees."
Federal law gives unaccompanied minors an automatic right to make a claim for asylum — even if the chances of having it granted are slim.
"Children are entitled to be heard on the question of asylum. But let's be honest … we can't expect a child to understand our asylum laws" and the requirements for making a claim, Leopold said.
Indeed, even for adults who meet one or more of the criteria to make a potential asylum claim, navigating the legal system is extremely difficult.
For most asylum seekers, the bar is extremely high. For instance, adults, or children who come to the United States with one or more parents, must demonstrate they are part of a targeted class that is suffering persecution at the hands of the government, or that the government is unable or unwilling to protect them.
Immigration courts require asylum seekers to provide concrete evidence of their claims. That means gathering documentation, affidavits, and other evidence.
And even if asylum seekers can put together a case, in many areas along the border — particularly in Texas — judges rarely grant asylum claims. In El Paso, for instance, immigration judges denied 87% of asylum requests between 2007 and 2012 — far higher than the national 50.6% average.
"Sometimes they question the authenticity of your documents, putting your whole credibility at risk," said Nancy Oretskin, an attorney with the Southwest Asylum and Migration Institute who works with asylum seekers held in detention centers in New Mexico and El Paso, Texas. "Winning an asylum case when one is in detention … it's incredibly difficult," Oretskin said.
For unaccompanied children, however, there is a second set of anti-human trafficking laws passed by Congress in the mid-2000s that provide a much broader set of potential protections, in asylum cases. Specifically designed to protect minors from traffickers, thousands of the children flooding into the U.S. could, in theory, be covered by this law "because it isn't persecution based," Leopold said.
But with so many minors making claims, there are concerns that the Border Patrol and others now tasked with assessing the asylum claims of undocumented immigrant minors are simply not up to the task.
Shifting members of the Department of Homeland Security's "Asylum Corps" — investigators trained in handling asylum cases from around the world — could help ensure minors' claims get a proper vetting because "there are serious questions about … the border patrol's ability to do this. And that's not a knock on the border patrol," Leopold explained.
"The most frustrating thing is that the law is so complicated in this area," Leopold said, warning that asylum cases can only be addressed on a "case-by-case basis," although that hasn't stopped politicians on both sides from capitalizing on the situation.
"You have politicians sound biting this in so many ways," Leopold lamented.
UPDATE 1:18 pm
White House spokesman Josh Earnest acknowledged Monday that the majority of the unaccompanied minor immigrants will end up being deported. "It's unlikely that most of the kids who go through this process will qualify for humanitarian relief, which is to say that most of them will not have a legal basis — will not be found, through that court process, to have a legal basis to remain in this country," Earnest said during his daily briefing with reporters.
Read more: http://buzzfeed.com/johnstanton/its-pretty-unlikely-the-undocumented-minors-will-be-granted


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Spontaneous antigen-specific T cell responses can be generated in hosts harboring a variety of solid malignancies, but are subverted by immune evasion mechanisms active within the tumor microenvironment. In contrast to solid tumors, the mechanisms that regulate T cell activation versus tolerance to hematological malignancies have been underexplored. A murine acute myeloid leukemia (AML) model was used to investigate antigen-specific T cell responses against AML cells inoculated i.v. versus s.c. Robust antigen-specific T cell responses were generated against AML cells after s.c., but not i.v., inoculation. In fact, i.v. AML cell inoculation prevented functional T cell activation in response to subsequent s.c. AML cell challenge. T cell dysfunction was antigen specific and did not depend on Tregs or myeloid-derived suppressor cells (MDSCs). Antigen-specific TCR-Tg CD8+ T cells proliferated, but failed to accumulate, and expressed low levels of effector cytokines in hosts after i.v. AML induction, consistent with abortive T cell activation and peripheral tolerance. Administration of agonistic anti-CD40 Ab to activate host APCs enhanced accumulation of functional T cells and prolonged survival. Our results suggest that antigen-specific T cell tolerance is a potent immune evasion mechanism in hosts with AML that can be reversed in vivo after CD40 engagement.
Long Zhang, Xiufen Chen, Xiao Liu, Douglas E. Kline, Ryan M. Teague, Thomas F. Gajewski, Justin Kline
Immune tolerance to transplanted organs is impaired when the innate immune system is activated in response to the tissue necrosis that occurs during harvesting and implantation procedures. A key molecule in this immune pathway is the intracellular TLR signal adaptor known as myeloid differentiation primary response gene 88 (MyD88). After transplantation, MyD88 induces DC maturation as well as the production of inflammatory mediators, such as IL-6 and TNF-α. However, upstream activators of MyD88 function in response to transplantation have not been identified. Here, we show that haptoglobin, an acute phase protein, is an initiator of this MyD88-dependent inflammatory process in a mouse model of skin transplantation. Necrotic lysates from transplanted skin elicited higher inflammatory responses in DCs than did nontransplanted lysates, suggesting DC-mediated responses are triggered by factors released during transplantation. Analysis of transplanted lysates identified haptoglobin as one of the proteins upregulated during transplantation. Expression of donor haptoglobin enhanced the onset of acute skin transplant rejection, whereas haptoglobin-deficient skin grafts showed delayed acute rejection and antidonor T cell priming in a MyD88-dependent graft rejection model. Thus, our results show that haptoglobin release following skin necrosis contributes to accelerated transplant rejection, with potential implications for the development of localized immunosuppressive therapies.
Hua Shen, Yang Song, Christopher M. Colangelo, Terence Wu, Can Bruce, Gaia Scabia, Anjela Galan, Margherita Maffei, Daniel R. Goldstein
Following organ transplantation, lifelong immunosuppressive therapy is required to prevent the host immune system from destroying the allograft. This can cause severe side effects and increased recipient morbidity and mortality. Complete cessation of immunosuppressive drugs has been successfully accomplished in selected transplant recipients, providing proof of principle that operational allograft tolerance is attainable in clinical transplantation. The intra-graft molecular pathways associated with successful drug withdrawal, however, are not well defined. In this study, we analyzed sequential blood and liver tissue samples collected from liver transplant recipients enrolled in a prospective multicenter immunosuppressive drug withdrawal clinical trial. Before initiation of drug withdrawal, operationally tolerant and non-tolerant recipients differed in the intra-graft expression of genes involved in the regulation of iron homeostasis. Furthermore, as compared with non-tolerant recipients, operationally tolerant patients exhibited higher serum levels of hepcidin and ferritin and increased hepatocyte iron deposition. Finally, liver tissue gene expression measurements accurately predicted the outcome of immunosuppressive withdrawal in an independent set of patients. These results point to a critical role for iron metabolism in the regulation of intra-graft alloimmune responses in humans and provide a set of biomarkers to conduct drug-weaning trials in liver transplantation.
Felix Bohne, Marc Martínez-Llordella, Juan-José Lozano, Rosa Miquel, Carlos Benítez, María-Carlota Londoño, Tommaso-María Manzia, Roberta Angelico, Dorine W. Swinkels, Harold Tjalsma, Marta López, Juan G. Abraldes, Eliano Bonaccorsi-Riani, Elmar Jaeckel, Richard Taubert, Jacques Pirenne, Antoni Rimola, Giuseppe Tisone, Alberto Sánchez-Fueyo
Chronic rejection, manifested as small airway fibrosis (obliterative bronchiolitis [OB]), is the main obstacle to long-term survival in lung transplantation. Recent studies demonstrate that the airways involved in a lung transplant are relatively hypoxic at baseline and that OB pathogenesis may be linked to ischemia induced by a transient loss of airway microvasculature. Here, we show that HIF-1α mediates airway microvascular repair in a model of orthotopic tracheal transplantation. Grafts with a conditional knockout of Hif1a demonstrated diminished recruitment of recipient-derived Tie2+ angiogenic cells to the allograft, impaired repair of damaged microvasculature, accelerated loss of microvascular perfusion, and hastened denudation of epithelial cells. In contrast, graft HIF-1α overexpression induced via an adenoviral vector prolonged airway microvascular perfusion, preserved epithelial integrity, extended the time window for the graft to be rescued from chronic rejection, and attenuated airway fibrotic remodeling. HIF-1α overexpression induced the expression of proangiogenic factors such as Sdf1, Plgf, and Vegf, and promoted the recruitment of vasoreparative Tie2+ cells. This study demonstrates that a therapy that enhances vascular integrity during acute rejection may promote graft health and prevent chronic rejection.
Xinguo Jiang, Mohammad A. Khan, Wen Tian, Joshua Beilke, Ramesh Natarajan, Jon Kosek, Mervin C. Yoder, Gregg L. Semenza, Mark R. Nicolls
Most degenerative diseases begin with a gradual loss of specific cell types before reaching a threshold for symptomatic onset. However, the endogenous regenerative capacities of different tissues are difficult to study, because of the limitations of models for early stages of cell loss. Therefore, we generated a transgenic mouse line (Mos-iCsp3) in which a lox-mismatched Cre/lox cassette can be activated to produce a drug-regulated dimerizable caspase-3. Tissue-restricted Cre expression yielded stochastic Casp3 expression, randomly ablating a subset of specific cell types in a defined domain. The limited and mosaic cell loss led to distinct responses in 3 different tissues targeted using respective Cre mice: reversible, impaired glucose tolerance with normoglycemia in pancreatic β cells; wound healing and irreversible hair loss in the skin; and permanent moderate deafness due to the loss of auditory hair cells in the inner ear. These mice will be important for assessing the repair capacities of tissues and the potential effectiveness of new regenerative therapies.
Masato Fujioka, Hisashi Tokano, Keiko Shiina Fujioka, Hideyuki Okano, Albert S.B. Edge
Transplantation of allogeneic stem cells into the early gestational fetus, a treatment termed in utero hematopoietic cell transplantation (IUHCTx), could potentially overcome the limitations of bone marrow transplants, including graft rejection and the chronic immunosuppression required to prevent rejection. However, clinical use of IUHCTx has been hampered by poor engraftment, possibly due to a host immune response against the graft. Since the fetal immune system is relatively immature, we hypothesized that maternal cells trafficking into the fetus may pose the true barrier to effective IUHCTx. Here, we have demonstrated that there is macrochimerism of maternal leukocytes in the blood of unmanipulated mouse fetuses, with substantial increases in T cell trafficking after IUHCTx. To determine the contribution of these maternal lymphocytes to rejection after IUHCTx, we bred T and/or B cell–deficient mothers to wild-type fathers and performed allogeneic IUHCTx into the immunocompetent fetuses. There was a marked improvement in engraftment if the mother lacked T cells but not B cells, indicating that maternal T cells are the main barrier to engraftment. Furthermore, when the graft was matched to the mother, there was no difference in engraftment between syngeneic and allogeneic fetal recipients. Our study suggests that the clinical success of IUHCTx may be improved by transplanting cells matched to the mother.
Amar Nijagal, Marta Wegorzewska, Erin Jarvis, Tom Le, Qizhi Tang, Tippi C. MacKenzie
Outcomes in transplantation have been limited by suboptimal long-term graft survival and toxicities associated with current immunosuppressive approaches. T cell costimulation blockade has shown promise as an alternative strategy to avoid the side effects of conventional immunosuppressive therapies, but targeting CD28-mediated costimulation alone has proven insufficient to prevent graft rejection in primates. Donor-specific memory T (TM) cells have been implicated in costimulation blockade–resistant transplant rejection, due to their enhanced effector function and decreased reliance on costimulatory signaling. Thus, we have tested a potential strategy to overcome TM cell–driven rejection by targeting molecules preferentially expressed on these cells, such as the adhesion molecule lymphocyte function–associated antigen 1 (LFA-1). Here, we show that short-term treatment (i.e., induction therapy) with the LFA-1–specific antibody TS-1/22 in combination with either basiliximab (an IL-2Rα–specific mAb) and sirolimus (a mammalian target of rapamycin inhibitor) or belatacept (a high-affinity variant of the CD28 costimulation–blocker CTLA4Ig) prolonged islet allograft survival in nonhuman primates relative to control treatments. Moreover, TS-1/22 masked LFA-1 on TM cells in vivo and inhibited the generation of alloproliferative and cytokine-producing effector T cells that expressed high levels of LFA-1 in vitro. These results support the use of LFA-1–specific induction therapy to neutralize costimulation blockade–resistant populations of T cells and further evaluation of LFA-1–specific therapeutics for use in transplantation.
Idelberto R. Badell, Maria C. Russell, Peter W. Thompson, Alexandra P. Turner, Tim A. Weaver, Jennifer M. Robertson, Jose G. Avila, Jose A. Cano, Brandi E. Johnson, Mingqing Song, Frank V. Leopardi, Sarah Swygert, Elizabeth A. Strobert, Mandy L. Ford, Allan D. Kirk, Christian P. Larsen
Rates of graft rejection are high among recipients of heart transplants. The onset and progression of clinically significant heart transplant rejection are currently monitored by serial biopsy, but this approach is highly invasive and lacks sensitivity. Here, we have developed what we believe to be a new technique to measure organ rejection noninvasively that involves the exploration of tissue-infiltrating leukocytes as biomarker sources for diagnostic imaging. Specifically, we profiled the myeloid response in a murine model of heart transplantation with the aim of defining and validating an imaging signature of graft rejection. Ly-6Chi monocytes, which promote inflammation, accumulated progressively in allografts but only transiently in isografts. Ly-6Clo monocytes, which help resolve inflammation, did not accumulate, although they composed the majority of the few remaining monocytes in isografts. The persistence of Ly-6Chi monocytes in allografts prompted us to screen for a Ly-6Chi monocyte–associated imaging marker. Low-density array data revealed that Ly-6Chi monocytes express 10-fold higher levels of myeloperoxidase (MPO) than Ly-6Clo monocytes. Noninvasive magnetic resonance imaging of MPO with an MPO-activatable Gd-chelate revealed a spatially defined T1-weighted signal in rejected allografts but not in isografts or MPO-deficient allograft recipients. Flow cytometry, enzymography, and histology validated the approach by mapping MPO activity to Ly-6Chi monocytes and neutrophils. Thus, MPO imaging represents a potential alternative to the current invasive clinical standard by which transplants are monitored.
Filip K. Swirski, Moritz Wildgruber, Takuya Ueno, Jose-Luiz Figueiredo, Peter Panizzi, Yoshiko Iwamoto, Elizabeth Zhang, James R. Stone, Elisenda Rodriguez, John W. Chen, Mikael J. Pittet, Ralph Weissleder, Matthias Nahrendorf
One of the main unresolved questions in solid organ transplantation is how to establish indefinite graft survival that is free from long-term treatment with immunosuppressive drugs and chronic rejection (i.e., the establishment of tolerance). The failure to achieve this goal may be related to the difficulty in identifying the phenotype and function of the cell subsets that participate in the induction of tolerance. To address this issue, we investigated the suppressive roles of recipient myeloid cells that may be manipulated to induce tolerance to transplanted hearts in mice. Using depleting mAbs, clodronate-loaded liposomes, and transgenic mice specific for depletion of CD11c+, CD11b+, or CD115+ cells, we identified a tolerogenic role for CD11b+CD115+Gr1+ monocytes during the induction of tolerance by costimulatory blockade with CD40L-specific mAb. Early after transplantation, Gr1+ monocytes migrated from the bone marrow into the transplanted organ, where they prevented the initiation of adaptive immune responses that lead to allograft rejection and participated in the development of Tregs. Our results suggest that mobilization of bone marrow CD11b+CD115+Gr1+ monocytes under sterile inflammatory conditions mediates the induction of indefinite allograft survival. We propose that manipulating the common bone marrow monocyte progenitor could be a useful clinical therapeutic approach for inducing transplantation tolerance.
Mercedes Rodriguez Garcia, Levi Ledgerwood, Yu Yang, Jiangnan Xu, Girdhari Lal, Bryna Burrell, Ge Ma, Daigo Hashimoto, Yansui Li, Peter Boros, Marcos Grisotto, Nico van Rooijen, Rafael Matesanz, Frank Tacke, Florent Ginhoux, Yaozhong Ding, Shu-Hsia Chen, Gwendalyn Randolph, Miriam Merad, Jonathan S. Bromberg, Jordi C. Ochando
Identifying transplant recipients in whom immunological tolerance is established or is developing would allow an individually tailored approach to their posttransplantation management. In this study, we aimed to develop reliable and reproducible in vitro assays capable of detecting tolerance in renal transplant recipients. Several biomarkers and bioassays were screened on a training set that included 11 operationally tolerant renal transplant recipients, recipient groups following different immunosuppressive regimes, recipients undergoing chronic rejection, and healthy controls. Highly predictive assays were repeated on an independent test set that included 24 tolerant renal transplant recipients. Tolerant patients displayed an expansion of peripheral blood B and NK lymphocytes, fewer activated CD4+ T cells, a lack of donor-specific antibodies, donor-specific hyporesponsiveness of CD4+ T cells, and a high ratio of forkhead box P3 to α-1,2-mannosidase gene expression. Microarray analysis further revealed in tolerant recipients a bias toward differential expression of B cell–related genes and their associated molecular pathways. By combining these indices of tolerance as a cross-platform biomarker signature, we were able to identify tolerant recipients in both the training set and the test set. This study provides an immunological profile of the tolerant state that, with further validation, should inform and shape drug-weaning protocols in renal transplant recipients.
Pervinder Sagoo, Esperanza Perucha, Birgit Sawitzki, Stefan Tomiuk, David A. Stephens, Patrick Miqueu, Stephanie Chapman, Ligia Craciun, Ruhena Sergeant, Sophie Brouard, Flavia Rovis, Elvira Jimenez, Amany Ballow, Magali Giral, Irene Rebollo-Mesa, Alain Le Moine, Cecile Braudeau, Rachel Hilton, Bernhard Gerstmayer, Katarzyna Bourcier, Adnan Sharif, Magdalena Krajewska, Graham M. Lord, Ian Roberts, Michel Goldman, Kathryn J. Wood, Kenneth Newell, Vicki Seyfert-Margolis, Anthony N. Warrens, Uwe Janssen, Hans-Dieter Volk, Jean-Paul Soulillou, Maria P. Hernandez-Fuentes, Robert I. Lechler
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In order to investigate the effects of exposure to buprenorphine compared with methadone during pregnancy, a prospective multicenter study was conducted in collaboration with maternity hospitals, maintenance therapy centers, and general practitioners involved in addiction care. Ninety pregnant women exposed to buprenorphine and 45 to metadone were selected for the study.
During pregnancy, some women were exposed to illicit agents: cannabis (42% in the buprenorphine group vs. 58% in the methadone-treated group), heroin (17% vs. 44%), or cocaine (3% vs. 11%). Pregnancies ended in 85 vs. 40 live births, one vs. two stillbirths, two vs. one spontaneous abortion, two vs. one voluntary termination, and one vs. one medical termination in the buprenorphine and the methadone groups, respectively. Newborns had a birth weight of 2,892 ± 506 g (buprenorphine) vs. 2,731 ± 634 g (methadone) and a body length of 47.6 ± 2.5 cm vs. 47.1 ± 3 cm. 18.8% vs. 10% of newborns were delivered before 37 weeks of amenorrhea. Neonatal withdrawal syndrome occurred more frequently in the methadone group (62.5% vs. 41.2, p = 0.03). After adjustment for heroin exposure in late pregnancy, rates of neonatal withdrawal were no longer different between the methadone and buprenorphine groups. Twenty-one babies (84%) in the methadone group and 20 (57%) in the buprenorphine group (p = 0.03) required opiate treatment.
We did not observe more frequent malformations or cases of withdrawal syndrome in the buprenorphine group than in the methadone-treated group. Buprenorphine appears to be as safe as the currently approved substitute methadone considered to date as the reference treatment for pregnant opioid-dependent women.
Centre Hospitalier Universitaire de Toulouse, Laboratoire de Pharmacologie Médicale et Clinique, Centre d'Evaluation et d'Information sur la Pharmacodépendance et d'Addictovigilance, INSERM U1027, Université de Toulouse, Toulouse, France, lacroix@cict.
This article was published in the following journal.
Name: European journal of clinical pharmacology
Comparison of Post-Cesarean Section Opioid Analgesic Requirements in Women With Opioid Use Disorder Treated With Methadone or Buprenorphine.
Buprenorphine is a highly effective treatment for opioid use disorders, but its continuation in the perioperative setting remains controversial, unlike the accepted practice of perioperative methadone...
Intravenous Buprenorphine: A Substitute for Naloxone in Methadone-Overdosed Patients?
Administration of naloxone is a common treatment for opioid-dependent patients who present with respiratory depression. Although safe in opioid-naive patients, naloxone may cause severe and even life-...
Pain Management in the Opioid-Dependent Pregnant Woman.
Opioid dependence is an epidemic in the United States, and the percentage of pregnant women who are opioid dependent has increased dramatically in the last decade. Pain management, already a concern f...
Frequency and correlates of sleep disturbance in methadone and buprenorphine-maintained patients.
Opioid use disorder (OUD) is a significant public health problem, and opioid maintenance treatment (OMT) on methadone or buprenorphine is a common approach. This study characterized sleep impairment i...
Fatal and non-fatal opioid overdose in opioid dependent patients treated with methadone, buprenorphine or implant naltrexone.
Illicit opioid use is associated with high rates of fatal and non-fatal opioid overdose. This study aims to compare rates of fatal and serious but non-fatal opioid overdose in opioid dependent patient...
RCT Comparing Methadone and Buprenorphine in Pregnant Women
Children born to women who abuse drugs have a high risk of being born with birth defects and developmental problems. Methadone is a drug that is commonly used for treating opioid dependenc...
Pilot Comparative Effectiveness Trial of Opioid Dependence Treatment Therapies in Pregnancy
The incidence of opioid dependence in pregnancy increased over the last decade from 1.2 to 5.8 per 1,000 hospital births per year.1 While methadone is the current, standard treatment for o...
Effects of Buprenorphine/Naloxone in Treating Opioid Dependent Individuals Who Are Maintained on Methadone
Buprenorphine is a drug that may be helpful in treating opioid dependent individuals who were previously maintained on methadone. The purpose of this study is to determine the effects of ...
Neonatal and Maternal Effects of Buprenorphine and Methadone
In this retrospective chart review, the investigators plan to analyze the effects of buprenorphine compared to methadone in opioid-maintained pregnant women. The investigators will evaluat...
Fetal Neurobehavior in Non-methadone Maintained Women in Substance Abuse Treatment
This study seeks to elucidate fetal neurobehavioral development in fetuses of opioid dependent women who have either undergone and completed methadone detoxification,or who never received ...
A synthetic opioid that is used as the hydrochloride. It is an opioid analgesic that is primarily a mu-opioid agonist. It has actions and uses similar to those of MORPHINE. It also has a depressant action on the cough center and may be given to control intractable cough associated with terminal lung cancer. Methadone is also used as part of the treatment of dependence on opioid drugs, although prolonged use of methadone itself may result in dependence. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1082-3)
Opiate Substitution Treatment
Medical treatment for opioid dependence using a substitute opiate such as METHADONE or BUPRENORPHINE.
A derivative of the opioid alkaloid THEBAINE that is a more potent and longer lasting analgesic than MORPHINE. It appears to act as a partial agonist at mu and kappa opioid receptors and as an antagonist at delta receptors. The lack of delta-agonist activity has been suggested to account for the observation that buprenorphine tolerance may not develop with chronic use.
Buprenorphine, Naloxone Drug Combination
A pharmaceutical preparation that combines buprenorphine, an OPIOID ANALGESIC with naloxone, a NARCOTIC ANTAGONIST to reduce the potential for NARCOTIC DEPENDENCE in the treatment of pain. It may also be used for OPIATE SUBSTITUTION THERAPY.
Pregnant Women
Human females who are pregnant, as cultural, psychological, or sociological entities.
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Fertility Menopause Obstetrics & Gynaecology Osteoporosis Women's Health Obstetrics and gynaecology comprises the care of the pregnant woman, her unborn child and the management of diseases specific to women. Most consultant...
Women's Health
Women's Health - key topics include breast cancer, pregnancy, menopause, stroke Follow and track Women's Health News on BioPortfolio: Women's Health News RSS Women'...
Collaborations in biotechnology
Commercial and academic collaborations are used throughout the biotechnology and pharmaceutical sector to enhance research and product development. Collaborations can take the form of research and evaluation agreements, licensing, partnerships etc. ...
Search PubMed Journal Article Database


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Tuesday 4 March 1997
It was Tuesday, under the sign of Pisces. The US president was Bill Clinton (Democratic).
Famous people born on this day include Alex Reininga .
In that special week of March people in US were listening to Wannabe by The Spice Girls.
In UK Where Do You Go by No Mercy was in the top 5 hits.
Donnie Brasco, directed by Mike Newell, was one of the most viewed movies released in 1997
while The Runaway Jury by John Grisham was one of the best selling books.
On TV people were watching Jai Hanuman.
If you liked videogames you were probably playing Atomic Bomberman or Formula 1 97.
But much more happened that day: find out below..
You can also have a look at the whole 1997 or at March 04 across the years.
Old Newspapers
Have a look at the old newspapers from 4 March 1997 and get them!
Which were the most popular movies released in the last months ?
TV Series
Which were the most popular TV series released in the last months ?
Find out your future
Get a FREE Numerology report based on the digits of 4 March 1997!
Wish him/her..
Historical Events
Which were the important events of 4 March 1997 ?
- Saint Efrem
- Catholicism - Commemoration of Saint Lucius I, pope, martyr.
- Saint Peter of Pappacarbone.
- Saint Lucius I.
- Saint Pierre de Cluny.
- Feast day of Humbert III of Savoy.
- Saint Basil and his Companions.
- Saint Adrian of Nicomedia, bishop of Saint Andrew's, and his Companions.
- Catholicism - Feast day of Saint Casimir, patron saint of Lithuania.
- United States - Inauguration Day (1789 - 1933)
- St. Thomas, Ontario, Canada - Charter Day (1881)
- Vermont - Admission Day (1791).
- Pennsylvania - Charter Day (1681).
- Wales - Feast day of Rhiannon, Celtic Moon Goddess.
- Feast day of Saint Placide Viel.
Famous Birthdays:
- Alex Reininga: YouTube Star.
Famous Deaths:
- Joe Baker-Cresswell: English Royal Navy officer (Born 1901).
- Edouard Klabinski: Polish professional racing cyclist (Born 1920).
- Comet Hale-Bopp directly above the Sun (1.04 AU).
- Zeya Start-1 launched (Russia).
- Brazil Senate allows women to wear slacks.
- President Clinton bans federally funded human cloning research.
- U.S. President Bill Clinton bars federal funding for any research on human cloning.
- The President of Guyana, Cheddi Jagan, dies in office.
- Pablo Picasso's ''Tête de Femme'' is stolen from a London gallery (recovered a week later).
- In Sri Lanka, Tamil Tigers overrun a military base and kill more than 200.
- India's Missionaries of Charity chooses Sister Nirmala to succeed Mother Teresa as its leader.
- The National People's Congress of the People's Republic of China creates a new Chongqing Municipality, out of part of Sichuan.
- The Phoenix Lights are seen over Phoenix, AZ.
Music Charts
Which were the top hits in that special week of March 1997 ?
Top #10 songs in the USA
- The Spice Girls - Wannabe
- Puff Daddy - Can't Nobody Hold Me Down
- Toni Braxton - Un-Break My Heart
- Jewel - You Were Meant For Me
- R. Kelly - I Believe I Can Fly
- Dru Hill - In My Bed
- En Vogue - Don't Let Go (Love)
- Babyface - Every Time I Close My Eyes
- Madonna - Don't Cry For Me Argentina
- Keith Sweat - Nobody
Search For Your Ancestors
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...and if 4 March 1997 was your Birth Date then Join our Birthday Club!
Make this date unforgettable
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Magazine Covers
What news were making the headlines those days in March 1997?
- Time: TIME Magazine Cover: Deng Xiaoping -- Mar. 3, 1997
- SportsIllustrated: Sports Illustrated: no.9576
- Life: Endangered Species Born In Zoos
Which were the most popular books released in the last weeks ?
Remember when
What videogames would you be playing back in the days ?


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A Chicago man is outraged about airport security after a disturbing incident that occurred this week while he was trying to fly home.
Brad Gertz says he was trying to catch an American Airlines flight out of Philadelphia. But when he tried to check in, he was informed someone else had been given his boarding pass and took an earlier flight.
Gertz was about to fly American's skies, when he was told he had already left.
They gave his boarding pass to someone else, he says.
That someone else got through security.
- Aviation expert Joe Schwieterman says the incident shows a breakdown of the system.
"Multiple failures had to occur for this passenger to get through with the wrong ID and when you let people through when they're not matching names, the whole essence of the system fails."
CBS 2 reached out to the TSA, which did not call back.


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Mumbai: The Bombay Stock Exchange's Sensitive Index, or Sensex, dropped 273.42, or to 12,886 at the close of trading today. The measure is set for its third weekly decline. The S&P/CNX Nifty Index on the National Stock Exchange fell 84.45, to 3,727.
Shares of ITC, India's biggest cigarette maker, dropped on concern the nation's 28 states may value-added tax (VAT) on tobacco products. Reliance and Larsen, India's biggest company and contractor, respectively, declined, as weaknesses in regional markets prompted some investors to sell local shares.
"While the India story is largely intact, investors are turning a bit cautious because emerging markets as an asset class are beginning to appear expensive," said K.K. Mital, who helps manage the equivalent of $32 million of Indian stocks at Escorts Asset Management in New Delhi. "Local factors are hurting stocks such as those of ITC. The extension of VAT to cigarettes has been talked about as a possibility."
Across the region, equities weakened on concern that the US economy, the world's biggest, will slow. The Morgan Stanley Capital International Asia-Pacific Index slid 0.8% to 142.34 at 6:08 p.m. in Tokyo. It has fallen 3.8% this week, the most since July 2006.
ITC dropped Rs5.95, or 3.5%, to Rs166.55. The tax on tobacco products will be imposed "after the central government direction comes," Ravi Mital, secretary, finance (commercial taxes) department, government of Bihar, an eastern state, said yesterday. States may charge the tax from 1 April, he said.
"While the excise rate hike of 5% on cigarettes was benign, potential additional tax burden due to VAT may be difficult to pass on, without sacrificing cigarette volume growth or margins in the near term," Princy Singh, an analyst at Citigroup Inc., wrote in a note to clients yesterday. He is maintaining a "sell" rating on the stock.
In its budget for the year starting 1 April, India increased excise taxes on cigarettes by 5%. Currently, India's states do not levy value-added tax on tobacco products. They apply VAT to at least 550 products. About 270 items such as medicines and farm supplies are taxed at 4 percent and the remaining at 12.5%.
The rate at which cigarettes may be taxed has not been decided.
Reliance fell Rs55.75, or 4.1%, to Rs1,311. Larsen declined Rs82.45, or 5.4%, to Rs1,458.1.


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Faith Based Roman Catholic Community
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Today's Readings
Opposite the Life of Christ windows on both sides of the church are the windows dedicated to the founders of religious orders.
St. Francis of Assisi
In the corner on the right, there is a window dedicated to St. Francis of Assisi.
He was born at Assisi, Italy, in 1182. After a carefree youth, he renounced his paternal wealth and committed himself to God. He led a life of evangelical poverty and preached the love of God to all. He established a rule, which his companions followed and which eventually gained papal approval.
Subsequently he founded an order of nuns and a society of laypersons who practice penance while living in the world. Later he received in his body the stigmata, i.e., the wounds of Jesus.
Surrounded by his friars, he died in 1226.
St. Francis is remembered for his love of nature. One of the legends about him is that he tamed a ferocious wolf in the village of Gubbio, Italy. The window shows St. Francis petting the wolf.
His feast is celebrated on October 4th. Sometimes pets and animals are blessed on his feastday.
St. Vincent de Paul
Beside the St. Francis window, there is a window dedicated to St. Vincent de Paul.
He was born in Gascony in 1581. After completiing his studies, he was ordained a priest and went to Paris where he served in a parish. He founded the Congregation of the Mission to supervise the formation of priests and to give support to the poor.
This order, often called the Vincentians, staffed St. Mary's Diocesan Seminary in Houston for many years.
With the help of St. Louie de Marillac, he also founded the Congregation of the Daughters of Charity.
He died at Paris in 1660.
St. Benedict
On the left side of the church in the corner facing the Life of Christ windows there is a window dedicated to St. Benedict.
He was born in Nursia, Unbria in Italy, about the year 480.
Educated in Rome, he began to live as a hermit at Subiaco where he eventually attracted disciples. He then departed for Monte Cassino. There he established the famous monastery and composed the Benedictine Rule.
Because this rule was subsequently adopted throughout Europe, he received the title of patriarch of Western monasticism.
He died on March 21, 547, but since the end of the eighth century, his feast has been observed on July 11th.
The window shows St. Benedict plowing, exemplifying the Benedictine motto, "
Ora et labora--Pray and work
St. Dominic
Next to St. Benedict, there is a window dedicated to St. Dominic.
He was born in Calaruega in Spain around the year 1170. He studied theology at Palencia and was made a canon of the church of Osma. He worked effectively against the Albigensian heresy through preaching and the rosary and good example.
To perpetuate this work, he gathered companions and founded the Order of Preachers (sometimes called the Dominicans).
He died at Bologna on August 6, 1221. His feast is celebrated on August 8th.


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Closing in 2 seconds...
The Global Pound Conference is your opportunity to be part of dispute resolution history. The London conference aims to explore how litigation, arbitration, mediation and other dispute resolution processes can help achieve greater user satisfaction. The day will be structured around four interactive sessions where core questions will be voted on and discussed in order to better understand the demand for dispute resolution services and what innovations might be beneficial. In both content and geographic reach it is a unique series of events unlike any other. Those participating - users, practitioners and other stakeholders - will be use dedicated technologies to gather data and different views.
We invite you to attend, debate with colleagues and hear from Corporate Counsel and other users, as well as respected experts who will share their own perspectives on the future.
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The conference, which will be opened by the Lord Mayor of London, is geared to corporate and public sector users of dispute resolution services (in house counsel and business leaders), external law firms and legal advisors, judges, neutrals, and ADR practitioners as well as key members of academia and government departments or agencies.
Chairs of the Local and Global Organising Committees - Dr Karl Mackie CBE and Michael McIlwrath
Key Note – Rt Hon Lord Justice Briggs Kt QC, Author of the Civil Court Structure Review.
Opening by the Lord Mayor of London, Alderman Dr Andrew Parmley
Master Ceremony: Natasha Johnson and Marc Stephens, PWC
Moderator: Alexander Oddy Head of ADR and Deputy Head of Commercial Litigation, Herbert Smith Freehills LLP, London
Moderator: Dr Karl Mackie CBE, Founding President, CEDR
Moderator: John J Fisher, Partner Forensic Services, PwC
Moderator: Marion Smith QC, 39 Essex Street & Trustee of the Chartered Institute of Arbitrators
Moderated by Michael McIlwrath, with Nadja Alexander, Barney Jordaan, Jeremy Lack, Debbie Masucci and Alexander Oddy
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When hearing an album nobody really wants to keep the experience feeling 'bummed out' or very introspective, that might most probably end up in them not hearing the cd again for a time or when they do being pensive when it extends to the ultimate few paths or in the event of the more (negatively) psychologically charged songs staying at the start they'd possibly skip them entirely.
That brings us to how to listen. Every great melody has items that are made to trigger an emotional effect. If you are hearing your preferred kinds, attempt to pay attention to which component causes you to respond the most. Subsequently, look at the different elements that increase that element. For example, what's the melody like? What're the background tools playing at the time? Or, what are the words?
This is actually the preamble for the chorus. It sets the arena, truly lyrically, so when the sentiments improvement they often tell a tale or recount attacks from a scenario while that's by no means important. They're usually seven or sixteen bars lengthy and melodically not often as sturdy while the chorus though, again, that's certainly not crucial.
Songs of relationship are comprised of terms, functions, actions, journeys, despair, pleasure and also the checklist is as long while you want it to extend. Do you realize your tune? Should you not, it could be time to remember then write your track.
The first 1960's also see-the start of the Angle dance phenomenon instigated by the substantial strike "The Twist" by Chubby Checker which dancing craze could continue until at the least 1962. 1962 might also see the beginning of Frankie Valli and also the Four Periods making use of their first 3 singles all achieving no 1 in the Usa ("Sherry", "Walk Just Like A Person" and "Big Girls Don't Cry").
When the cause individual was inside the center of the cd the probabilities are that the crowd will skip straight to it and then end up playing the following songs. This may imply a sizable piece of content will undoubtedly be overlooked while in the first listen through. Now that you have read this far, has that stirred your opinions in any way? There is a great deal within the body of knowledge surrounding mp3 search songs . Yes, it is correct that so many find this and other related subjects to be of great value. A lot of things can have an effect, and you should widen your scope of knowledge. Try evaluating your own unique requirements which will help you further refine what may be necessary. We will tie everything together plus give you a hint of other important information.
Once the music stops and we lose our associate, buddy, lover the sting, harm must be treated by you. A relationship expands predicated on shared desires, destinations, interest and affection, all excellent seeds of the wonderful track. A Broken Heart can sometimes heal using the help of a mutually shared understanding of what designed the relationship, we are calling it Your Song. We could assist you with all the feelings, the thoughts, the feeling of sadness and loss in self respect.
Melodically, the verse keeps an all natural, conversational move. Every passage will keep the exact same tune, allowing for little adjustments to support the lyric.
The tune composition might not be the first thing you think about if you start publishing. You probably focus on the passage or chorus, or maybe you have an excellent coat that you want to increase in to a track. So you get that along and after that you start to think about the the rest – the introduction, how many sentiments, middle seven, do you want a crucial, the ending…
It is their shows about the exhibit that kick started Beatlemania in the United States along with the remaining portion of the world. No additional group had previously induced so much hysteria no additional group has done since. The Beatles turned mega stars using their silly notes, stunning melodies, gathering incredible industrial and important achievement and 20 no 1 visits in the Usa in just 6 years. No other performer or collection has been ready to fit their success since and it's really unlikely anyone ever will.


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2011: The Year in Books
I did it. I read fifty books this year. After 2010's embarrassing performance, I'm rather proud of myself, especially since that fifty includes some really long ones like Jonathan Strange & Mr. Norrell and 1Q84 and some really hard ones like The Satanic Verses and Midnight's Children. I enjoyed the vast majority of them, and… Read More 2011: The Year in Books


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Discussion in 'Introductions' started by Jenwren, 9th Jan, 2017.


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Figure of Speech | Trevor Wheatley
INTERVIEW: Chais Mingo | PHOTOGRAPHY: Jake Sherman
Dialogue | Issue 02 | p.101
Both provoking and unreal, Trevor Wheatley has drawn audiences in with his incredibly surreal typographic sculptures that explore contemporary culture. Working as a team alongside Cosmo Dean whom helps create and conceptualize the pieces, and Jake Sherman who stunningly captures the results on camera, allowing the work to be shared online for those who aren't lucky enough to stumble upon the works in their obscure natural environments.
Trevor's roots are in graffiti so it comes as little surprise that he prefers to present his work in the public realm. However for his latest work the canvas is not usually the urban landscape. Rather the group utilizes contemporary slang or recognizable corporate logos, and takes them out of their familiar urban setting into the empty and natural wilderness. The large and at times intricate creations offer a striking juxtaposition between urban and rural, past and present, and nature and technology. This natural environment allows the viewers to appreciate the work on their own terms and creates a dialogue outside the narrow confines of a gallery.
It's hard not to be envious of the group of friends and collaborators. Working together on concepts and designs in their Toronto based studio and then venturing into the woods or off to a foreign country with the ultimate goal of re-assembling and documenting their work. Adventure is a key part of the process and the group often sets out without any particular destination. The crew and their work are often at the mercy of Mother Nature, yet the team embraces the uncertainty of working in the outdoors and allows the documentation to occur naturally. The setting is often as important as the sculpture as it allows for new interactions between the environment and the artwork.
For their latest project the crew took a trip to California to set up a new installation in the distinctive desert landscape of Joshua Tree National Park. We caught up with Trevor, Cosmo, Jake on the road to hear more about their process and inspiration.
What was the catalyst for working predominantly with sculptures?
T: When I graduated from art school I started working on film sets. The bigger films had huge budgets and invested heavily in their standing sets. After a few shoot days the sets were disassembled and thrown out. I'd go into the dumpsters and fill my trunk every evening. It was an inexpensive way to start making sculptures, I'd make text pieces that were comprised of many materials from different films or tv series. Cosmo had ten years of building experience, so when the work and the installation rigs became more complex we began to work together.
Please explain the creation process of yourself and your team.
T: Generally Cosmo and I will bounce ideas off one another until we run out of time and both have to make some creative concessions. No two projects have ever been the same, so each one presents new conceptual and material issues. We source materials from a wide range of places, and more recently have been working with different fabricators. Including water cutters, laser cutters, steal welders, even ice sculptors. Once the sculpture is made we go on a location scout with Jake Sherman and plan where the work will live and try to predict the look it will have. Only so much planning can be done, as weather often dictates the results of the installation and shoot days.
Your work often features current slang and lingo usually found only in pop culture, the Internet and casual conversation. Why do feel it's necessary to give permanence and physical form to ephemeral language?
C: We don't view the language we choose as being inherently ephemeral. Definitely it has contemporary relevance as slang, but much of the text we've done also has an alternate definition- if not a classical dictionary definition, at least a contrasting definition that isn't necessarily how it's predominantly used in pop culture. We use the slang to make it relatable and accessible, but appreciate the duality in the language as it allows us to point at bigger societal issues, often evident in the more 'ephermal' definitions of the words we tend towards.
How do you think your work will be viewed 10 years into the future?
T: We've been talking a great deal about permanence and it's importance to our practice moving forward. Over a short period we've traveled extensively and made a portfolio that is now acting as a catalyst to get projects that will be permanent. We feel strongly that you have to earn that permanence, and recently have been getting offers to create more timeless sculptures. In the summer we will be working on a few permanent pieces, it will be interesting to see how that will affect the work.
Do you think it matters or are you more concerned with the "here and now?"
C: Our hope is to create a sustainable working art practice for ourselves, so remaining current and relevant will always be a part of that. Although we do think a lot about more permanent and timeless sculptures we plan to make, our current focus is on evolving quickly and presenting a unique reflection of society as it's happening.
Your work lends itself easily to branding and commercial interests. How do you balance the often conflicting aims of art and commerce?
C: We've come to see the commercial work as something of a blessing, and it makes it feel like less of a compromise and more of an accommodation of our personal practices. Definitely we have to make some concessions, but the aesthetic and tactile elements of our practice are something we enjoy very much, so doing work that doesn't necessarily align with our personal conceptual considerations isn't entirely un-enjoyable.
What happens to your work after the installations are completed?
T: Initially we would leave the work where it was installed. When we went back to document it as it aged and eroded we worried about the environmental implications the sculptures were creating. More recently we take the work back to the studio, where we have been hoarding text in many different typefaces, sizes, styles, and materials. Down the road we plan on using them for a text cage piece that will float in the sky.
Your work often finds you in rural areas or trekking through the wilderness, sometimes in the cold of Canadian winters. What are some of your favorite or most memorable installations whether good or bad?
J: I think that one of the fondest memories we mutually share would be the snitch install. It wasn't necessarily the most extreme to date, but it has the glory of being one of the first projects we did that was an absolute battle to capture. The scale of the work was huge, and it was finished quality, so the physical act of carrying and placing the sculpture was a feat in itself, and then we got caught deep in the woods in a pretty bad winter storm. We were racing against time and ended up trekking out in the dark of night. The whole thing was just so all encompassing that when it was over the sense of accomplishment was really euphoric. Second would probably be real talk. We had a whole gang of friends with us for that one, over the course of three days. It was just a leisurely pleasure. Almost the opposite actually.
How do you go about location scouting? Have you run into issues with property owners?
J: We all go out hiking together, with Jake being the final say in what the actual frame will be. Usually we have a vague idea of the landscape were looking for, but we like to make a day of it, weighing our options and figuring out what's realistic in terms of time and transport. Occasionally we don't have the luxury of time, and we just set out with the work on our backs and hope for the best. Often times this garners some of the strongest results, and definitely can be an exciting way to do things. Our motto with property owners or local authority tends to be a combination of beg for forgiveness rather then permission, and pleading ignorance. We aren't particularly invasive, and we're happy to oblige anyone who doesn't want us around by simply leaving politely, so thus far we've been fortunate to not have had any major altercations. Our experience is that most people are happy to let you use their space when you explain the purpose and intention, and most are even eager to accommodate and help however they can.
Can you give us the story behind your concept for this latest series in the Californian desert?
T: Each landscape presents different possibilities, it's less about the desert and more about shooting in as many interesting locations as possible. The sculptures interact with different environments in varying ways and it's exciting for us to open the work up to different possibilities. If we were constantly shooting locally the work would be in danger of becoming redundant, so travelling has become an important part of our practice. Generally we don't pick the text until we arrive on location, and everything is made on site. Travelling dictates the content, the look, the scale, and the final imagery produced.
You can find the full print version of this interview in issue 02 of our magazine | shop now


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The last decades the disease Lyme borreliosis that is spread by ticks has been increasing, but this increase cannot be explained by the increasing deer population only.
Results from the research project TickDeer, recently published in Nature Communications, shows that the percentage of ticks with Borrelia is decreasing in areas with a high deer population (deer, red deer and moose). However, the total number of ticks is higher.
This means that the risk of catching Borrelia is not decreasing as much as one could hope for despite the "clean host," and all in all a high density of deer give a netto increase in borreliosis.
Since 1991 Norway has registered all incidents with borreliosis in a so-called MSIS-statistics for reportable diseases.
At the same time data on the wild deer population over all of Norway has been recorded. This is why Norway has a unique set of data material for comparing the relation between the disease progression over time, says professor Atle Mysterud, who led the project.
In addition the researchers at the TickDeer-project, which represent a cooperation between the Department of Biosciences (University of Oslo), Norwegian Veterinary Institute, and NIBIO, looked at how the number of deer affects the number of ticks, and in particular ticks with Borrelia. The results show that borreliosis has increased more than the density of deer would suggest.
"We see that the incidence of borreliosis has increased in the southern part of Norway in a period of time where both the deer population and the moose population have decreased. This is why we cannot only blame the deer for getting more of these blood-sucking parasites," says professor Atle Mysterud.
"At the west coast an increase in the population density of deer has resulted in an increase in the incidence of borreliosis. The cycle of the Borrelia bacterium in nature is both complex and is influenced by many different drivers, where the hosts of the ticks in an early life stage can influence the cycle. There is also little doubt that a warmer climate is a contributing factor for the tick-borne diseases, something that we are going to have a closer look at in the newly started #EcoTick-project," says Mysterud.
"It is also likely that the change in the use of the landscape, resulting in more encroachment, has been an advantage for the ticks."
Ixodes ricinus, the tick that is known for infecting humans in Norway as well as in rest of Europe, has a life cycle that is threefold; larva, nymph, and adult ticks.
It needs a blood meal in each of the life stages, but they may have different hosts at different stages.
- In the first stage it is rodents and birds that become victims of these parasites.
- In the second stage they usually suck blood from slightly larger animals.
- It is in the last stage that the tick is an adult and needs blood for reproduction. Then the host is usually a large animal like deer. Deer are therefore frequently referred to as reproductive hosts, and are thought to be important for the population dynamics of the ticks.
Ticks are born pure. That is why the hosts in the first life stage of the tick determine whether it will be a carrier of infection or not.
Ticks can therefore only be infected in the second and third stage.
In the third stage, they are quite large, and we often feel that they crawl on the skin. Therefore nymphs are more often inflicting infection, because we do not notice their bite.
In the TickDeer-project the researchers have looked at the populations of deer and counted the ticks on the animals and in the landscape by so called "flagging." In addition, they took samples of the ticks to determine how many of them that contained Borrelia.
Deer contributes to keep the ticks clean since they are not carriers of the Borrelia-bacteria. Therefore ticks that have used deer as a host do not contain Borrelia that can infect us.
A dense population of deer contributes overall to more cases of Lyme disease. This is mainly because the deer are a good host for the ticks. However, the relationship is not very strong.


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Statistics for occurrence #1 of "Barbadoes" in chapter 59 of Admiral David D. Porter, The Naval History of the Civil War.:
... Home Government limited the supply of coal to what was supposed to be necessary to enable a Confederate cruiser to reach one of the ports of the Confederacy. From Nassau the Florida proceeded to Barbadoes , where she received on board one hundred tons of coal, in further violation of the orders of the Home Government, which provided that a second supply of coal should not be allowed within three months...
|Max. Freq.
|Min. Freq.
† This entity has been selected by the automated classifier as the most likely match in this context. It may or may not be the correct match.


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Eric Alexander Salon Brand - hours and store locations
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We stayed here for 3 nights in total and recommend it highly. We were staying in a private room for the stay, but moved into a much nicer one for the last evening. The first room (130 Qs) is a very basic room but ticked...More
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This property has been removed by the agent. It may be sold or temporarily removed from the market
5 bedroom detached house for saleKingsley Way, Whiteley
Sold STC £495,000
Property Description
Key features
- Tucked away in corner of cul-de-sac
- Five bedrooms with two en-suites
- Tandem length garage
- South facing private garden
- Kitchen & dining room to rear
- Two further reception rooms
- Spacious entrance hallway
- Utility room & cloakroom
- Master room on whole of top floor
- Juliet style balcony on landing
Full description
Nestled away in the corner of the ever growing popular location of Whiteley is this spacious five bedroom detached house. This popular design offers accommodation split over three floors with a healthy balance of space distributed throughout; on the ground floor is a welcoming entrance hall providing access to two large reception rooms looking out to the wooded frontage, a large kitchen & dining room to the rear, utility room & cloakroom. On the first floor are four bedrooms with one bedroom boasting an en-suite and the main family bathroom; the entire top floor encompasses the master room with its own large dressing area and en-suite with double shower. The property enjoys a secluded position with wooded areas to the front and side of the property, approached via a double length driveway leading to a tandem garage and a private south facing rear garden. Being situated off the Sweethills Crescent part of Whiteley, the property sits within catchment of Whiteley Primary School and Cornerstone Primary School. Furthermore, the newly revamped and thriving Shopping Centre are also within close walking distance. To make an arrangement to view, please contact SBK on 01489 570019 or firstname.lastname@example.com.
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Disclaimer - Property reference 6962043. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by SBK Property Consultants, Park Gate - Sales. Please contact the selling agent or developer directly to obtain any information which may be available under the terms of The Energy Performance of Buildings (Certificates and Inspections) (England and Wales) Regulations 2007 or the Home Report if in relation to a residential property in Scotland.
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text: How to Win at Scrabble with Word Descrambler Trying to impress your anagram-loving, board game-playing date? Look no further... more »Trying to impress your anagram-loving, board game-playing date? Look no further than assisted success with Word Descrambler for iPhone, iPad, and iPod Touch. http://itunes.apple.com/app/id379792391 « less nheagy July 25, 2010 351 Views More Info Full Credits nheagy Uploader Stats & Data 3Funny1Die 351 Views July 25, 2010 Published Category Parody Tags scrabble date cheat iphone advertisement Transcript Ah, a candle-lit game of Scrabble. You Romantic. But be careful: if you don't win she won't be impressed. And she's leading by thirty points? That won't work. First, pretend to cough. Now, duck under the table and silently pull out your iPhone running Word Descrambler. Enter your letters - what was that last one? Right. There's a great word. Play it. Haha! Now you've destroyed her dreams and secured your victory. If that doesn't win her over, nothing will. WORD DESCRAMBLER. Also available as a free version, you love fool. Up Next: iPhone 4 Video - The Deleted Scenes AUTOPLAY Up Next In: 10 iPhone 4 Video - The Deleted Scenes Mike and Dave Need Wedding Dates | Red Band Trailer - In Theaters July 8th Bobby needs a date Mike and Dave Need Wedding Dates Trailer Follow Browse Related Comments Vaguely Related Videos Vaguely Related Pics & Words Advertisement Tags: scrabble date cheat iphone advertisement Advertisement


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Williams Lea Profits on Growth
From http://www.timesleaderonline.com
WHEELING — Williams Lea and its Wheeling office is poised to weather the storm of a challenging economy and capitalize on opportunities for growth and expansion.
Williams Lea is a global business process outsourcing company focused on providing corporate information solutions. The company provides expert production to delivery management of all printed and digital information an organization shares with its internal and external audiences.
In 2005, Williams Lea established an outsource hub in Wheeling at the former Stone & Thomas department store building on Main Street in downtown. Wheeling's operating model is based on "the centralization of support services, leveraging an attractive cost base, unwavering work ethic and effective workflow management."
The very nature of Williams Lea's service offering enables the company to continue to provide business process outsourcing to companies all over the world.
As more and more companies seek out opportunities to manage their cost base more effectively and streamline their internal processes, Williams Lea is working to provide innovative outsourcing solutions.
Williams Lea's history of providing value-added services to its clients enables it to engineer cost-saving business solutions for the growing pool of companies looking for ways to increase net worth while decreasing spending. For the Wheeling office of Williams Lea, this means untold potential growth.
Williams Lea Vice President of Document Services John Snowdon said the success of the Wheeling office is due to a significant labor pool that is well-educated and has a strong work ethic.
"Our region's pool of highly skilled, dedicated workers coupled with the relatively low cost of doing business will enable Williams Lea Wheeling to offer significant cost savings to clients in more expensive metropolitan areas," Snowdon said.


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Erik Wahlberg, Impact! Kickboxing Fitness' black-belted owner and trainer, carves confident, fit figures by drawing on more than two decades of martial-arts experience and conditioning know-how. The 90-minute classes, which take place Monday, Wednesday, and Sunday ($12 each), incorporate cardio training, core exercises, and thorough assaults on the gym's collection of heavy bags. Atop the kickboxing mecca's multihued mats, students will master moves, pummel calories, and build muscle mass. Impact's supportive, motivation-inspiring instructors will help swift-kicking students augment their fighting techniques faster than a cheetah on rollerblades.
CoupFlip is a marketplace for people to buy and sell deals from "daily deal" (also known as "group buying") sites such as Groupon. On CoupFlip you can buy deals you missed, look for interesting deals in your area, or sell any deal you bought and won't use. The purchase is immediate - pick the deal, pay, download the voucher instantly and enjoy. Every voucher is guaranteed to be authentic by CoupFlip. For sellers, we provide the opportunity to sell your unwanted deal immediately without the hassle of listing your voucher and praying someone comes along and decides they feel like buying it.
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By Kenneth Silber |
August 1, 2008
On November 17, 1881, German Kaiser Wilhelm I issued an imperial decree stating that "those who are disabled from work by age and invalidity have...
By Mark Tibergien |
December 1, 2005
Hurricane Katrina triggered this most recent reflection. My dad and stepmother have lived on the Mississippi Coast for the past 20 years. Now in their...
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- Worldwide Rank #45,183,073
- Follower/Following Ratio 1.36
- Daily New Followers 0
bark.. Twitter Stats
@007_bark - Tracking bark.. Twitter profile since November 27, 2012
Bark Selangkah Di depan'Mu.
bark..'s story
bark.., also known as @007_bark has a reasonably significant presence on Twitter and is ranked by us in the 30% percentile for account strength. Active on Twitter since April 2012, bark.. made it to having a respectable 255 Twitter followers and to being ranked 45,183,073 for number of followers among all Twitter users.
Uh-oh... seems like someone has fallen asleep while on duty and is dropping in social relevance. The plot thickens when considering bark..'s follower-to-following ratio, which is 1.36. That's a healthy ratio.
Tweeting is apparently one of @007_bark's favorite pastimes, with an average of 0 tweet(s) per day in the past 30 days. That's pretty consistent with a total of 8,788 since @007_bark joined Twitter.
This account is tracked by us for quite some time now, actually since November 2012. As of August 18 2017 we track 279120400 Twitter accounts, so feel free to search for other accounts you're interested in and reveal their Twitter story!
bark.. Tweets
@007_bark has posted 0 tweets in the last 30 days, which translates to an average of 0 tweets per day.
bark.. Twitter Followers
@007_bark has 255 followers on Twitter.
This account is #45,183,073 in the worldwide rank of the most popular Twitter users.
bark.. Following on Twitter
@007_bark is following 187 Twitter accounts.
Last month this account followed 66 users.
bark.. Predictions & Milestones
@007_bark will hit 255 followers in the next 3 months, and 255 in one year.
You are on bark..'s Twitter stats page
We track these Twitter stats since November 27, 2012 . You can see how many followers bark.. lost or gained and what the prediction is for tomorrow or the next 15 days, together with all kinds of other stats like rank compared to all Twitter users, tweets etc.
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Twitter Counter is the #1 Twitter stats site powered by Twitter. It all started in June 12, 2008 and we grew rather rapidly because of our Twitter Counter badge. The button is a nice small image that shows your visitors how many followers you have on Twitter. Within a year the button was on many many blogs around the world and we displayed more than 100 million buttons in one month. We track follower stats for over 279.120.400 users now.


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Once Made in the U.S. Jobs Trickle Back to the U.S.
Some U.S. businesses are bringing their jobs back to the U.S. from China. This is a welcome change to the fledging U.S. manufacturing industry, and many hope it is a long-term trend. So far, however, numbers suggest the absolute new jobs created this way in the U.S. is still a drop in the ocean.
Dragon Baby Too Lucky to Leave to Chances
What does every aspiring dragon mother want? A dragon baby. In the year of the dragon, some Chinese and Chinese-Americans are so committed to welcoming a child this year that they are getting fertility treatments to boost their chances.
Unleashing Innovation in China
China has had an image that it is a technology "copy cat" rather than an innovator. This is changing, especially in certain industries where the government sees that domestic consumption will be the growth engine for the future.
As China's Income Gap Grows, Tombs Are a Target
As China's economic boom lifts people's standard of living, the disparity between the rich and the poor also widens. According to the world bank, China's income inequality is now on par with some Latin American and African countries. In a society where huge income inequality is only a recent phenomenon, any display of such difference creates imbalance, anger and potentially social unrest. The government is taking measures to limit the public display of such inequality.
Over the Lunar New Year weekend Vivian Choi made her annual visit to Wong Tai Sin, one of Hong Kong's largest Taoist temples, to ask for blessings in the new year. But instead of burning dozens of incense sticks in the age-old Taoist tradition, Ms. Choi slipped a written prayer into a small box. An electronic deity statue then lit up and blew artificial smoke, signaling the acceptance of the offering.
So a Chinese Guy Walks into a Bar, and says He's Irish - Get it?
A successful Chinese American comedian who wowed audiences in the United States (including Vice President Joe Biden) gets no laughs in China for his jokes. The Chinese audience simply did not find his jokes funny.
Improved Cross-Cultural Communication Increases Global Sourcing Productivity, Accenture Study Finds
The study found that executives believe adopting cross-cultural communication training programs can increase productivity by 26 percent, on average. Two thirds of all respondents said they had experienced miscommunication issues within their global operations.