text:
Fitness has always been a big part of my life but nothing could prepare me for the challenge of motherhood!! Fit4Mom has been a life changer. It has given me confidence, strength and community. And the best part is that I can have all that with my two little boys, Ray (4) and Marcus (19 months) at my side. They are wonderful and inspiring kids. I started Fit4Mom when my second was only 5 months. Immediately I knew that this had to be a bigger part of my life. Now, as the co-owner of Fit4Mom Conejo, I am inspired to help other mothers feel, experience and enjoy life through fitness, friendship and fun!more
I started Stroller Strides when my daughter was 9 weeks old and I couldn't fit into a single dress in my closet. Fast forward eight months and I feel strong, fit and confident. I can do more squats, lunges, and crunches than ever before. I even ran a 5K with very little training other than the morning classes. More importantly, I've made friendships with amazing mamas and their babies and have so much fun and learn so much from them. I don't feel like my day is complete without going to Stroller Strides and I'm sad if I have to miss a day. Who would have thought that a workout could be the highlight of your day?
I went into my closet the other day to see what fit for an upcoming wedding, and to my surprise, every single dress fit perfectly. I wasn't spending hours a day in the gym and I wasn't on any crazy diet. I was doing exactly what I had done before with one change. Stroller Strides. It has made an incredible difference in my life, both physically and emotionally, with fitness that helps me to feel confident and happy and friendships that I know will last a lifetime.more
My name is Annie and I have 2 boys, Sawyer who is almost 4 and Graham who is almost 2. There will be another little boy joining the crew in December 2016. I started Stroller Strides when Sawyer was 8 weeks old while living in San Diego. We moved to the Los Angeles area and I found Fit4Mom Conejo when I was 5 months pregnant with Graham and have been hooked ever since! Not only did I find an amazing group of women who were going through the journeys of motherhood together but also like minded regarding health and fitness. As far as the workout is concerned, I found what works for my body. Things I worked years to try and change, have changed! A combination of resistance training and cardiovascular work has proved to be the key to maintaining a strong and healthy body that I can be proud of!
My college education in Food Science led me to a career in weight loss and most recently as a physical education and health science teacher ranging from grades K-12. Becoming an instructor for Fit4Mom gave me back that piece of ME that I lost when I started having babies. I absolutely LOVE teaching and unlike most high school students you Mamas want to be there! I have a passion for health and fitness and being able to share that with this group is very special to me. I am beyond grateful to have found this village!more
I am mommy to my sweet girl Anniston (2). I grew up as a ballet dancer so I have always been passionate about the body and about fitness through all forms of movement, which led me to obtain a degree in Kinesiology-exercise science. I discovered Fit4mom Conejo when my daughter was 3 months, and fell in love with everything it represents...strength, love, community. To meet other mamas working towards our shared goal of a healthy and fit lifestyle, while spending precious time with our children, and setting an awesome example for them is priceless. I am so happy to be part of this amazing team.more
My name is Tanya and I am a first time mom to bebe Xavier (13 months). I am extremely passionate about sport and athletics and being physically active has always been a large part of my life. In the past, I competed in long distance outrigger canoe races and am still an avid gym goer, hiker, runner and cyclist. I moved to Southern California from Canada when I was pregnant with Xavier and started Stroller Strides when he was 6 months old. I was hooked from the first class. When I joined, my main goal was to meet other mommas in the area and I was pleasantly surprised when I discovered that Stroller Strides was not only a tight-knit community of mommas, but also a fun and challenging workout! I love teaching Xavier about leading a healthy lifestyle while also getting my sweat on! When I had the opportunity to become an instructor, I jumped at it. I believe wholeheartedly in the fit4mom and fit4baby philosophy as I've seen the positive results time and time again. Whether you've always been active or you're just getting back into the swing of things, Stroller Strides works for all fitness levels. I am inspired to help you reach your fitness goals whatever they may be and to introduce you to a 'village' of wonderful mommas! Strong moms creating strong bonds, what's not to love!more
I hope you enjoy reading all the fun activities we plan each month cause I have a blast writing them. Besides being your "sister-in-sweat" I am known as the "meet up mom".
My pre-motherhood resume includes a AS from Moorpark College and pending BS in Political Science from CSUN. A career that includes public speaking, corporate training and area supervision for multi-family housing kept me very busy before switching gears to be a stay at home mom to Soli (4) and Steele (2). Descriptive writing and storytelling are second nature for me.
Fitness has also served a meaningful role in my life. From starting the first women's lacrosse team in high school to creating a company wide fitness program to improve the overall health of the employees; bringing health and wellness to those around me is important.
I am eternally grateful for the strong and loving women that have become some of my closest friends through Fit4Mom. Being part of the team is an honor and a privilege.
Our Village & Craft Mamamore
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Discrete wavelet transform for signal de-noising to enhance the accuracy of fiber-optic Weight-In-Motion(WIM) system is presented in this paper. The fiber-optic WIM system working principle is demonstrated and the characteristic of the axle weight signal is analyzed. The algorithm of the discrete wavelet transformation and its implementation is discussed in… (More)
With the development of the internet, many important documents can be exchanged online. It is vital to protect the safety and genuineness of the seals in the published documents. In this paper, a new electronic seal system based on the RSA digital signature algorithm and public-key infrastructure is presented. The RSA digital signature algorithm and its… (More)
A new kind of coupling fiber-optic vibration sensor whose sensing mechanism based on the characteristic of the coupler's coupling output ratio sensitive to the length variation of coupling zone is presented and demonstrated in this paper. The working principle of coupling fiber-optic vibration sensor is analyzed and a prototype system including a… (More)
- LI Xiao-mim, Ma Zhi-yuan, Xu Jian
- 2011 Third International Conference on Measuring…
- 2011
The number of foreign professional digital tester designed for frequency characteristic is not large and the cost is too high as well. What's more, homemade equipments are complex and bulky with low-accurate attenuator and no phase-frequency characteristic testing functions. To solve these problems, we studied on some key techniques such as the… (More)
Safety and genuineness protection of the documents while traveling over the internet is very crucial. In this paper, a novel watermarking scheme for electronic seal based on discrete wavelet transform (DWT) is proposed. The theory of digital watermark based on DWT for electronic seal is discussed. The watermark signals are considered as a binary sequence… (More)
In order to optimize the trade-off between performance and security of digital signature in electronic commerce; an electronic seal system based on elliptical curve cryptosystem is presented. Elliptical curve cryptosystem is adopted as it is an effective system which has the advantage of fast speed and high security. The identity-based scheme is introduced… (More)
A new kind of coupling single-mode fiber-optic sensor used the coupler's coupling region as sensitive unite is presented. The theory of coupling single-mode fiber-optic sensor is analyzed. According to the relation between the coupler's coupling ratio with the length and refractive index of its coupling region, strain and vibration measuring technology with… (More)
An innovative low-frequency fiber-optic coupling vibration sensor for monitoring vibration of large-size structures is presented. The working principle of low-frequency fiber-optic coupling vibration sensor is discussed and the demodulation system for photoelectric conversion and signal process to its response is designed. A prototype fiber optic vibration… (More)
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subset: commoncrawl


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Listen to free music played by zondabez
Blip.fm is internet radio made social. It's easy to search for, play, and discover free music recommended by real people. Join today to create your own free station and start listening to @zondabez.
zondabez {arriba! hola, lola!} #lolaflores
Lola Flores – Lola de España| play
Jan 20, 2011 at 8:22 pm via YouTube | AMAZON | ITUNES
amphore gave props on Dec 11 at 6:11 pm
zondabez "sun+moon+stars" {looking 4 kelly} RT @NaturallyZen (reblip)
Thievery Corporation – Sun Moon And Stars| play
Jun 25, 2011 at 8:00 pm via YouTube | AMAZON | ITUNES
StreamItRadio gave 2 props on Jun 25 at 1:12 pm
Freestyle – The Party's Just Begun (1990)| play
Jun 25, 2011 at 8:55 pm via YouTube | AMAZON | ITUNES
StreamItRadio gave props on Jun 25 at 3:38 pm
zondabez #musicfriday {é isso, vida coceira!} #eutohqtoh
Apr 15, 2011 at 3:57 pm via YouTube | AMAZON | ITUNES
StreamItRadio gave props on Jun 25 at 1:12 pm
zondabez dance a modern dance #sundaybeats #luscofusco #bsb #finde
Pere Ubu – The Modern Dance| play
tassiac gave props on May 10 at 9:08 am
zondabez RT @revista_trip: "Pra animar esta #FridayFriday, um clássico de Wilson Pickett, que faria 70 anos hoje se estivesse vivo!" (reblip)
Wilson Pickett – Land of 1000 Dances| play
zondabez {porque hoje sim é dia bom pra se fazer um gol!!} #musicmonday #homemgol
Debora Blando – Decadence Avec Elegance| play
Mar 18, 2011 at 6:45 pm via YouTube | AMAZON | ITUNES
enrique_ gave props on Mar 18 at 11:46 am
zondabez @rapozo: "Erasmo Carlos – Agora ninguém chora mais" {assim é bem melhor...} #tremendao #classics #vix (reblip)
Erasmo Carlos – Agora ninguém chora mais| play
luiz_com_z gave props on Jan 15 at 6:12 pm
rapozo gave 2 props on Dec 3 at 11:18 am
zondabez porque as camadas do tempo se sobrepõem e o ontem vai ser sempre hoje: amanhã, que forma tens?! #horrorpop
HorrorPops – Everything's Everything| play
Oct 17, 2010 at 7:57 pm via YouTube | AMAZON | ITUNES
Maripiazzolla gave props on Oct 17 at 7:49 pm
The Naked and Famous – The Sun| play
Oct 17, 2010 at 8:07 pm via YouTube | AMAZON | ITUNES
santamistura gave props on Oct 17 at 1:11 pm
zondabez "i wanna be with u everywhere..." {nice fleetwood mac cover by vampire weekend} #musicsunday
StreamItRadio gave props on Aug 28 at 7:33 pm
zondabez pow, ela é mó baranga...mas a música é mó grudenta...."just like u knew u would..." #ugotmegood
neozeitgeist gave props on Jul 18 at 10:09 pm
StreamItRadio gave props on Jul 18 at 6:57 pm
zondabez @mark_till: "Simple Minds Alive and Kicking" #musicmonday #80sgoodvibe (reblip)
mark_till gave props on May 24 at 8:24 am
May 24, 2010 at 2:56 pm via YouTube | AMAZON | ITUNES
mark_till gave props on May 24 at 7:57 am
djordjesavic gave props on Mar 8 at 3:32 pm
zondabez RT @violenced: "good one! rb@patita: "i like this vibe!!!!!!!!!!! #musicmonday (reblip)
violenced gave props on Mar 8 at 9:12 am
C&C Music Factory – Here We Go| play
QueenzQT gave props on Mar 6 at 5:51 pm
zondabez ...deu saudade da 'tia' hoje... #sweetandmellow #musicwednesday
Elton John – Rocket Man, Elton John| play
neozeitgeist gave props on Mar 4 at 8:36 am
zondabez hast du lili heute getroffen?
neozeitgeist gave props on Feb 25 at 7:27 am


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This past year saw a substantial growth in the amount of cargo handling in the ports of Sultan Qaboos and Salalah despite the fact that the actual number of vessels moored at both ports saw a decline.
During the first quarter of 2014 Port Sultan Qaboos (PSQ) and Port of Salalah
experienced growth of handling of cargo by 12.7 percent at PSQ and an impressive 58 percent at Salalah port.
The National Center for Statistics and Information reported that the total volume of loaded and unloaded export cargo increased from 1.26 million tonnes during the first quarter of 2013 to 1.42 million tonnes by the end of March 2014. At Salalah port the report stated that 1.69 million tonnes were handled during the first three months of 2013 compared to 2.68 million tonnes during the same period in 2014.
Interestingly, the number of mooring vessels docked at PSQ and Salalah ports saw a shrinkage of 6.5 percent; 973 vessels at the end of March 2013 down to 910 vessels.


text:
|A cup of tea please, Agatha. I'll also have strawberries, whipped cream and crumpets.
New York: Peter Som for Anthropologie
|Let's run through the meadow in the Bois de Boulogne. A picnic basket will be waiting for us at the end of the rainbow.
2. Les Envies for Nina Ricci
| I'm off to lunch for some pink ice cream and a mint julipe. Have the driver pick up Pepper as well. She just loves pink ice cream.
3. Paris: Cacharel Spring 2013
|There is something very charming about the fire swamp: flame spurts, ligthening sand and rodents of unusual size. Come save me Westley.
4. New York: Caroline Herrera Spring 2013
|I almost bought some pastel trousers but I chose a book instead.
5. London: Clement Ribeiro Spring 2013
|Welcome to my world bitches. Get me my latte, extra hot.
6. New York: DKNY Spring 2013
|Even thought they smell like petting zoos, take me to a renaissance fair darling.
7. Paris: Isabel Marant Spring 2013
| que linda Conchitta and don't forget to cover my grave in red roses
8. Berlin: Lena Hoschek Spring 2013
| a dogged follower of fashion without caring one single bit. We love it.
9. Paris: Louis Vuitton Spring 2013
Nine dresses and nine things to do while wearing them.


text:
Las Penitas, Nicaragua
Las Penitas, Nicaragua
Las Penitas is a charming, historic resort and fishing village on the northwest Pacific coast of Nicaragua. The 1.5 mile sandy beach is the undisputed main attraction. Know for is mellow vibe, Las Penitas attracts surfers year around and body boarding is also becoming increasingly popular. Several hostels can arrange too horse back rides on and off the beach. Fishermen offer boat tours to neighboring destinations, mainly to the Juan Venado Island. Located just off the coast in front of Las Peñitas, it is an important nesting ground for sea turtles and is also home to crocodiles, caimans, birds, crabs, and much more.
Share the post "Las Penitas, Nicaragua"
About Leticia Davila
Leticia Davila studied Business Administration in English at the University in Granada, and later traveled abroad to complete more intensive studies in Business and English. She began to seriously pursue her passion for teaching the language, culture of traditions of Nicaragua in 2000, and is currently a teacher and the administrator of Spanish Dale! Language School.


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Farah Khan
Farah is a second-year student in the MA program in Criminal Justice and specializes in Correction Administration and Juvenile Justice. She received a BA degree in Sociology with a minor in Women's Studies from Hunter College and has been working in a non-profit after school program for the last six years. Her area of interest is in the management of correctional settings for juvenile offenders. Farah completed CRJ848 Public Health Challenges
in Criminal Justice: An Epidemiological Approach in Fall 2011.


text:
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Research Associate.UHC General Lab-41000
(posted: 8/15/2017)
The research assistant will apply principles and knowledge relevant to research projects in the field of molecular biology and immunology........
University Hospitals
| Cleveland, Ohio, United States
Bilingual (Mandarin / English) Legal Assistant
(posted: 8/15/2017)
Wong and Associates is a national immigration law practice representing clients throughout the United States. Perform basic legal research........
Margaret W. Wong & Associates, LLC
| Cleveland, Ohio, United States
Research Associate
(posted: 8/15/2017)
The Research Associate will be responsible for working closely with the PI to develop scientific research projects in the area of cancer........
University of Kentucky
| Lexington, Kentucky, United States
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Candidate must develop and maintain a good working relationships, and be adept at dealing with program team members and support department personnel........
Lockheed Martin
| Lexington, Kentucky, United States
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(posted: 8/15/2017)
As a Retail Service Operations associate you will review and process. Limited Term Humana associates may work........
| Louisville, Kentucky, United States
Professional Associate II
(posted: 8/15/2017)
In the interest of maintaining a safe and healthy environment for our students, employees, patients and visitors the University of Kentucky is a Tobacco & Drug.......
University of Kentucky
| Lexington, Kentucky, United States
Program Specialist (IRB Coordinator)
(posted: 8/15/2017)
Provides guidance regarding categories of research that are exempt from committee review, or that qualify for expedited review........
Veterans Affairs, Veterans Health Administration
| Lexington, Kentucky, United States
Research Associate/COM
(posted: 8/15/2017)
The Center for Health Services Research is seeking a Research Associate. Utilize research programs on health economics........
University of Kentucky
| Lexington, Kentucky, United States
Research Associate Clinical I
(posted: 8/15/2017)
The Markey Cancer Center ( MCC ) department of Core Support is seeking a Research Associate Clinical I ( CRA I) to work with the MCC Early Therapeutics study.......
University of Kentucky
| Lexington, Kentucky, United States
HR Call Center Specialist - Bilingual in Spanish candidates only
(posted: 8/15/2017)
And online travel, expense and purchasing systems. Provide guidance to Humana associates requesting information about and assistance with:........
| Louisville, Kentucky, United States
Medical Assistant - Research Recruitment Specialist
(posted: 8/15/2017)
Scheduling subjects for research study visits using computer scheduling software (GroupReady). Ensure compliance with applicable protocols, as well as the.......
L-MARC Research Center
| Louisville, Kentucky, United States
Payment/ARM Specialist II
(posted: 8/15/2017)
Travel between Republic Banking facilities may be required. Verify billing notices have generated and are mailed to clients according to regulatory guidelines........
Republic Bank
| Louisville, Kentucky, United States
Client Service Associate I
(posted: 8/15/2017)
Works under direct supervision to provide unlicensed support in all phases of client service, client relationship building, and account maintenance to assist.......
Hilliard Lyons
| Lexington, Kentucky, United States
Patient Services Representative
(posted: 8/15/2017)
The world-class physicians at Retina Associates of Kentucky provide excellent patient care in a compassionate manner, are engaged in major research projects and.......
Retina Associates of Kentucky
| Lexington, Kentucky, United States
Ophthalmic Technician II
(posted: 8/15/2017)
The world-class physicians at Retina Associates of Kentucky provide excellent patient care in a compassionate manner, are engaged in major research projects and.......
Retina Associates of Kentucky
| Lexington, Kentucky, United States
Learning Technology Consultant - Louisville, KY
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Provide routine backup and storage of instructional materials, programs and research information. Investigate and resolve specific problems, emerging learning.......
| Louisville, Kentucky, United States
Info & Referral Techn U315
(posted: 8/15/2017)
Researches publications and databases for information on social service programs and refers clients to appropriate social service resources........
Louisville Metro Government
| Louisville, Kentucky, United States
Inventory Research Clerk
(posted: 8/15/2017)
Familiar with basic computer skills � including Windows based programs, hand tools, packaging materials, etc�. Research and correct mispicked invoices........
| Bowling Green, Kentucky, United States
Office Coordinator, CHHS Dean's Office
(posted: 8/15/2017)
Western Kentucky University does not discriminate on the basis of race, color, national origin, sex, sexual orientation, gender identity, disability, age,.......
Western Kentucky University
| Bowling Green, Kentucky, United States
Associate Underwriter I-IV
(posted: 8/15/2017)
Associates Degree or equivalent experience. The division is currently in need for an Associate Underwriter who will assist the underwriting staff within a.......
Great American Insurance Group
| Lexington, Kentucky, United States
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text:
[Highest rank: #12 in Humor]
"I can't go around walking wearing one shoe. I would look stupid," she mumbled looking elsewhere, skillfully trying to avoid my gaze.
"Well in that case," I said as I crouched down and started taking off my shoes. "Let's look stupid together."
Life as told by a Guy.
Book Two: Beyond His Flaws
TheSaltineCracker Apr 16
That's my brother's birthday and my best friends mom's birthday
fallen_angelinluv Aug 06, 2016
My mom does that. She takes away the blanket in the winters so that I can wake up! It's a nightmare!
Lievergent Sep 07, 2016
I am with you! I too love sleep and I am pretty darn amazing at it😂
XWild_HeartX Dec 28, 2016
Hey :) I just noticed your name and tbh that's my nickname at home :)))


text:
A driver's license belonging to Paula Broadwell was turned in on Sunday to the Maryland-National Capital Park Police, a park police spokesperson has confirmed to TPM.
Earlier Tuesday, U.S.News & World Report first reported that the license had been found by a jogger in Rock Creek Park in Washington D.C. Lt. Bill Kellogg, the Park Police spokesman, also told TPM that the park police notified the FBI on Tuesday about the license's discovery.
On Friday, Broadwell was identified as the woman with whom former CIA director Gen. David Petraeus had been carrying on an affair. Kellogg said it was all the attention surrounding Broadwell that prompted his agency to contact the FBI.
"To us it was nothing suspicious," Kellogg said. "It was just lost property that was turned in."
As of Tuesday afternoon, the Park Police was still in possession of the license.


text:
Owner & Consultant
David Doughty Media & Marketing
Created marketing material for Dawn Food Products, Inc.
Created marketing video for Oman Ministry of Health.
Work on websites and create digital marketing strategies for clients.
Social Media Director & Website Design
Career Development Services LLC
- Create and execute Social Media campaigns for consultants, job seekers, and small businesses
- Design and build websites for individuals and small businesses
New Media Coordinator & Co-Producer
Media Production Group
- Created 3-5 min. marketing videos of different non-profit work around the world.
- Created and worked on multiple marketing campaigns and participated in strategic leadership development.
- Created promotional pieces for different city councils; in-house videos for larger manufacturers; produced low budget commercials; and non-profit promo videos.
Director & Video Coordinator
University of Central Oklahoma
Athletic Video Coordinator
Created weekly video updates at bronchosports.com and recruiting videos. Also was in charge of multimedia operations at the football stadium.
Consistently taught student interns how to run big screen at game and operate cameras.
News Director, KCSU-TV
Taught other students how to run a live student-run newscast.
Teacher's Assistant for a class and often took have the class to teach while the professor took the other half of the class.
Experienced Directing, Technical Directing, Audio Engineering, Floor Managing, and running cameras for student run newscast that went live at 5pm every weekday.
Music Director, KUCO-FM
In charge of teaching students how to use the sound booth and scheduled air time for each one that needed it for school credit.
In charge of entire operation at radio station including play list reporting to College Music Journal, contacting labels and promoters, and producing the morning show


text:
19 Sep 2014: The PLOS ONE Staff (2014) Correction: A Functionally Significant Polymorphism in ID3 Is Associated with Human Coronary Pathology. PLOS ONE 9(9): e109222. https://doi.org/10.1371/journal.pone.0109222 View correction
We previously identified association between the ID3 SNP rs11574 and carotid intima-media thickness in the Diabetes Heart Study, a predominantly White diabetic population. The nonsynonymous SNP rs11574 results in an amino acid substitution in the C-terminal region of ID3, attenuating the dominant negative function of ID3 as an inhibitor of basic HLH factor E12-mediated transcription. In the current investigation, we characterize the association between the functionally significant polymorphism in ID3, rs11574, with human coronary pathology.
Methods and Results
The Multi-Ethnic Study of Atherosclerosis (MESA) is a longitudinal study of subclinical cardiovascular disease, including non-Hispanic White (n = 2,588), African American (n = 2,560) and Hispanic (n = 2,130) participants with data on coronary artery calcium (CAC). The Coronary Assessment in Virginia cohort (CAVA) included 71 patients aged 30–80 years, undergoing a medically necessary cardiac catheterization and intravascular ultrasound (IVUS) at the University of Virginia. ID3 SNP rs11574 risk allele was associated with the presence of CAC in MESA Whites (P = 0.017). In addition, the risk allele was associated with greater atheroma burden and stenosis in the CAVA cohort (P = 0.003, P = 0.04 respectively). The risk allele remained predictive of atheroma burden in multivariate analysis (Model 1: covariates age, gender, and LDL, regression coefficient = 9.578, SE = 3.657, p = 0.0110; Model 2: covariates Model 1, presence of hypertension, presence of diabetes, regression coefficient = 8.389, SE = 4.788, p = 0.0163).
We present additional cohorts that demonstrate association of ID3 SNP rs11574 directly with human coronary artery pathology as measured by CAC and IVUS: one a multiethnic, relatively healthy population with low levels of diabetes and the second a predominantly White population with a higher incidence of T2DM referred for cardiac catheterization.
Citation: Manichaikul A, Rich SS, Perry H, Yeboah J, Law M, Davis M, et al. (2014) A Functionally Significant Polymorphism in ID3 Is Associated with Human Coronary Pathology. PLoS ONE 9(3): e90222. https://doi.org/10.1371/journal.pone.0090222
Editor: Antonio Abbate, Virginia Commonwealth University, United States of America
Received: November 13, 2013; Accepted: January 27, 2014; Published: March 6, 2014
Copyright: © 2014 Manichaikul et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The CAVA study was supported by NIH P01 HL55798 (CAM), NIH R01 HL096447 (CAM), NIH R01 HL107490 (CAM), K23HL093118-04 (AMT), Pfizer Preventive Cardiology Award (AMT). The funders of the CAVA study had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. MESA and the MESA SHARe project are conducted and supported by contracts N01-HC-95159 through N01-HC-95169 and RR-024156 from the National Heart, Lung, and Blood Institute (NHLBI). Funding for MESA SHARe genotyping was provided by NHLBI Contract N02-HL-6-4278. MESA Family is conducted and supported in collaboration with MESA investigators; support is provided by grants and contracts R01HL071051, R01HL071205, R01HL071250, R01HL071251, R01HL071252, R01HL071258, R01HL071259. The funders (NHLBI) had a significant role in design and data collection for the MESA study. The funders had no role in analysis, decision to publish, or preparation of the manuscript.
Competing interests: Coleen McNamara and Angela Taylor have an investigator-initiated research grant with Astra Zeneca. A portion of the work was also supported by a Pfizer Preventive Cardiology Award (PI: Angela Taylor). This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.
Coronary artery disease remains the leading cause of death in the United States and most developed countries. Despite rapid advances in therapies aimed at decreasing atherosclerotic disease, significant cardiovascular risk remains. Thus, it is critical to translate novel murine findings into humans with the ultimate goal of creating novel therapies that further reduce cardiovascular risk. To do so requires careful phenotyping of human atherosclerosis at the level of coronary artery pathology. Intravascular ultrasound (IVUS), an invasive imaging study, has become the gold standard for defining the extent of human coronary atherosclerotic burden. While quantitative coronary angiography detects only late disease as evidenced by luminal stenoses, IVUS can directly and precisely quantify disease burden within the artery wall even prior to luminal compromise or presence of calcification [1]. Further, disease burden as measured by IVUS is predictive of cardiovascular events and has also emerged as a strong surrogate marker for events [2], [3]. Coronary artery calcium (CAC) as measured by computed tomography (CT) has emerged as a strong noninvasive surrogate marker for disease burden and event risk. Indeed, in intermediate risk populations, CAC has greater predictive ability than other noninvasive predictors of cardiovascular risk [4].
Research over the last decade has enjoyed major breakthroughs in our understanding of atherosclerotic cardiovascular disease [5]–[8]. Yet, most of these novel insights have come from studies in genetically modified mouse models and corroboration of such pathways in human atherosclerotic disease has been lacking [9]. Sequencing of the human genome and the explosion of the field of human genetics provides opportunities to explore genes implicated in atherosclerosis pathogenesis in murine models in human studies. Statistical associations of single nucleotide polymorphisms (SNPs) with disease provide support that these genes may be involved in human disease pathogenesis [10]. Yet, most SNPs are located in non-coding regions making it difficult to study the relationship between the SNP, the functional impact of the SNP, and disease pathogenesis.
The dominant negative helix-loop-helix (HLH) transcription regulator, ID3, has emerged as an important upstream regulator of atheroprotective pathways in immune and vessel wall cells [11]–[13]. Identification of a SNP in the coding region of the human ID3 gene, associated with subclinical atherosclerosis in the Diabetes Heart study [11], raises the interesting possibility that ID3 may have a role in human atherosclerotic disease. This nonsynonymous SNP results in a marked attenuation in the ability for ID3 to affect downstream transcription [11]. Importantly, the allele frequency of the SNP is as high as 48% in White diabetic populations, 28% in HapMap Whites (CEU) and 30% in HapMap Mexican (MEX) populations. ID3 and the bHLH factors it regulates have been implicated in the regulation of gene pathways that mediate cellular growth, differentiation, survival and inflammatory gene expression, supporting the notion that a functional SNP in this upstream pathway regulator could impact on a complex polygenic process such as atherogenesis.
We previously identified an association between the ID3 SNP rs11574 and carotid intima-media thickness (cIMT) in a predominantly White diabetic population. Here we present two additional cohorts that demonstrate the association of the SNP directly with human coronary artery pathology as measured by CAC and IVUS: the first a multiethnic, relatively healthy population with low levels of diabetes and the second a predominantly White population with a higher incidence of T2DM referred for cardiac catheterization.
Ethics statement
All MESA participants gave written informed consent, including consent to participate in genetic studies. The MESA study was conducted under Institutional Review Board approval at all study sites, including the Cedars-Sinai Medical Center and the University of Virginia. Written informed consent was obtained for all participants of the CAVA study, which was performed under protocol approved by the University of Virginia Institutional Review Board. All methodology was compliant with the principles set forth in the Declaration of Helsinki.
MESA Study Design
The Multi-Ethnic Study of Atherosclerosis (MESA) is a longitudinal study of subclinical cardiovascular disease that seeks to determine which risk factors predict progression of subclinical disease or progression to clinically overt cardiovascular disease [14]. 6,814 participants free of clinically defined cardiovascular disease were recruited from six field centers across the United States from 2000–2002. Participants were categorized into race/ethnic groups by self-report, and 38% of the recruited participants are non-Hispanic Whites, 28% African-American, 22% Hispanic, and 12% Asian, predominantly of Chinese descent.
Participants recruited by the MESA cohort (6,814) and two ancillary studies – MESA Family Study (MESAFS with 2,128 participants from 528 families) and MESA Air (5,479 participants from MESA, 257 external to MESA, and 490 from MESAFS) were genotyped in 2009 using the Affymetrix Human SNP array 6.0 (∼1 million SNPs). Genotyping and quality control are described in greater detail in the Supplementary Methods in File S1.
CAVA Study Design
The Coronary Assessment in Virginia cohort (CAVA) included 71 patients between the ages of 30 and 80 undergoing a medically necessary cardiac catheterization. Patients were excluded if they had any of the following: a current acute coronary syndrome, cancer of any type, autoimmune disease of any type or on immunosuppressive therapy, prior organ transplantation, anemia, pregnancy, HIV infection, or no vessel suitable for IVUS. All protocols and procedures were approved by the Institutional Review Board at the University of Virginia (IRB HSR #14620).
Phenotyping of MESA and CAVA participants
The MESA cohort measures of subclinical atherosclerosis (SCA) included the presence or absence of CAC, as a dichotomous phenotype. Participants were also assessed for risk factors of subclinical atheroslcerosis, diabetes status, and hypertension.
For CAVA participants, intravascular ultrasound in accordance with the standards of the American College of Cardiology for image acquisition on a non-infarct related artery [15]. The first 40 mm of the artery were used for analysis. The LAD was preferentially analyzed unless there was a contraindication such as tortuosity. Plaque and cross-sectional area were performed at each millimeter and atheroma burden was calculated per segment. Quantitative coronary angiography was also performed for each of the CAVA participants, and percent stenosis was determined using automated edge-detection software by a blinded reader.
Details regarding the phenotyping for MESA and CAVA participants are included in the Supplementary Methods in File S1.
Statistical analyses in MESA and CAVA
For MESA, the SNP rs11574 was imputed based on the genome-wide genotype data, and statistical association was assess for rs11574 with CAC, after adjustment for age, sex, study site and principal components of ancestry. For IVUS, rs11574 was genotyped directly and statistical analysis was performed with adjustment for age, gender, LDL, hypertension, and diabetes. Details for the statistical analyses are presented in the Supplementary Methods in File S1.
Characteristics of the study samples
Baseline characteristics and atherosclerosis risk factors for MESA Whites and CAVA participants are summarized separately by ID3 SNP rs11574 genotype in Table 1. Similar measures are also shown for MESA African American and Hispanic American participants in Table S1 in File S1. While the MESA Whites and CAVA are comparable in terms of gender and age distributions, the MESA participants are relatively healthier with notably lower BMI (median 26.9 for ID3 rs11574 risk allele carriers) compared to CAVA (median 32.1 for carriers). Prevalence of diabetes and hypertension are also lower in MESA compared to CAVA. MESA participants have higher LDL levels than CAVA, reflecting the fact that only about 20% of MESA participants are on lipid lowering medication, compared to 77% of CAVA participants on statins. Among MESA participants, the prevalence of CAC was highest in Whites (58.9% of risk allele carriers, Table S1 in File S1) compared to African Americans (49.4% of risk allele carriers) and Hispanics (46.3% of risk allele carriers).
ID3 SNP rs11574 and CAC in MESA
Our previous work indicating association of cIMT with rs11574 was completed in the Diabetes Heart Study, an entirely White cohort. Therefore, we designated analysis of MESA Whites as our primary analysis, and further examined association in other race/ethnic groups from MESA in secondary analyses. We performed genetic association analysis of the ID3 SNP rs11574 with measures of SCA in MESA with race/ethnic stratified analyses in White, African American and Hispanic participants, as well as a combined analysis across race/ethnic groups by meta-analysis. We observed statistically significant association of rs11574 with CAC in MESA Whites using a basic regression model that included covariates age, gender, study site, and PCs of ancestry (Table 2, P = 0.017). This effect was maintained in an extended regression model that added LDL as a covariate (P = 0.012). Although we did not observe statistically significant association of rs11574 with CAC in MESA African American or Hispanic cohorts, the direction of effect was the same across all three ethnic groups, such that we observed statistically significant association of ID3 rs11574 with CAC in meta-analysis across ethnic groups (P = 0.017 under the basic regression model, P = 0.016 after further adjustment for LDL).
ID3 SNP rs11574 and atheroma burden in CAVA
Given that CAC is a predictor of coronary disease burden, we sought to determine directly if the ID3 SNP rs11574 is associated with atheroma burden measured by IVUS and with stenosis severity by QCA in a second cohort. A representative IVUS and QCA from patients with and without the risk allele are shown in Figure 1. Subjects with the risk allele demonstrated a significantly greater atheroma burden than subjects without the risk allele (P = 0.003). Additionally, while QCA is a cruder measure of disease burden and has less ability to describe the full extent of disease, there was also a significant difference in stenosis between groups (P = 0.04) (Figure 2). Minimal lumen area also differed significantly between groups (Risk allele (6.13+/−3.02, Ancestral allele 4.37+/−2.07; p value 0.0158). Multivariate analysis was performed with 2 models as shown in Table 3. The risk allele remained the most predictive covariate of atheroma burden in multivariate analysis (Model 1 covariates: age, genotype, gender, and LDL, p = 0.0110, regression coefficient = 9.578, SE = 3.657; Model 2 covariates: Model 1, presence of hypertension, presence of diabetes, p = 0.0163, regression coefficient = 9.294, SE = 3.767, p = 0.0163).
Light green represents fibrofatty plaque, dark green fibrous plaque, red necrotic core, and white calcium. (bottom panel) Representative QCA from a patient with the ancestral allele with a 28% stenosis (left) and a patient with the risk allele and stenosis of 73% (right). Automated edge detection is used to determine stenosis severity.
Burden differed significantly between groups with greater burden in the presence of the risk allele. Stenosis was marginally significant. Vertical line represents complete spread of the data. Burden represents the percent of the vessel wall occupied by plaque. Stenosis represents percent luminal stenosis.
Atherosclerosis is a complex disease linked to both environmental and genetic risk factors. Population studies support an important role for environmental factors, most notably a Western lifestyle [16], [17]. Yet, atherosclerosis is clearly a heritable disease [18] and identification of gene variants associated with atherosclerosis holds promise to lead to novel biomarkers and new treatment strategies based on previously unknown regulatory pathways. In order to study the complexities of human coronary artery atherosclerosis and to investigate novel murine findings in the human model, one must have a reliable manner of phenotyping disease that is predictive of coronary events.
IVUS has emerged as the gold standard for measuring atheroma burden in a precise manner. IVUS has the ability to quantitate total atheroma volume in the artery wall whether or not it is visible on angiography. This allows for study of not only overt coronary artery disease but also earlier stages of disease that are otherwise not easily detectable. It is not surprising that the rs11574 SNP predicts early disease as represented by burden rather that late disease as measured by stenosis as other factor such as shear stress and endothelial damage may be at play in the presence of late, hemodynamically significant lesions. Atheroma burden by IVUS has been linked strongly to clinical events, even more so than traditional, well-accepted clinical risk variables [2], [3]. Noninvasively, both cIMT and CAC have been shown to be predictive of coronary disease events and may be population dependent in their predictive ability. CIMT appears to be most predictive in White diabetic populations while CAC is most predictive in healthier populations at moderate rather than high risk for coronary events such as that found in MESA [4]. Thus, the proper choice of surrogate endpoints may depend on the stage of disease being studied and the population in which it is being studied. Careful phenotyping can provide a model whereby associations of coronary disease with novel pathophysiologies discovered in murine models can be initially tested in humans.
Genome-wide association studies (GWAS) have allowed for analysis of millions of genotyped SNPs as well as additional imputed SNPs to identify variant gene loci associated with increased risk for coronary artery disease. However, because GWAS applies an unbiased approach to discovery of novel genomic variants, they employ very strict thresholds for statistical significance that account for multiple testing across millions of SNPs. By their nature, GWAS studies fail to take into account the wealth of existing knowledge in biological associations, from both human and animal studies, that have accumulated over the past several decades of research. Here, we utilized a more targeted approach, testing for association of one SNP in a gene that is shown to be atheroprotective in multiple mouse models of atherosclerotic disease, and associated with cIMT in a cohort of White individuals with type 2 diabetes. Previous studies using genome-wide linkage analysis in humans and an interspecific genetic cross in mice identified risk for early MI and atherosclerosis associated with loci in which ID3 resides [19], [20]. Testing only for association of the ID3 SNP rs11574, we found association of this SNP and coronary artery disease in two cohorts; CAC in MESA and IVUS coronary artery plaque volume in CAVA, a cohort of White patients undergoing cardiac catheterization.
Comparing the results across race/ethnic groups in MESA, we found the strongest association for Whites, with weaker evidence for association in African Americans and Hispanics. The direction of effect for the rs11574 SNP was observed to be consistent across all race/ethnic groups, suggesting a common functional mechanism lies behind the association in all three groups. The difference in statistical significance across MESA race/ethnic groups may be due in part to reduced frequency of the risk allele in MESA African Americans (6.1%) and Hispanics (14.6%) as compared to MESA Whites (23.9%). The attenuated effect estimates seen in African Americans and Hispanics also indicate there may be other genetic and environmental factors modifying the association between the ID3 SNP rs11574 and CAC in MESA participants. For example, behavioral and dietary differences across race/ethnic groups may overlap with the functional pathway of the ID3 SNP, leading to differences in SNP effects across these groups. In addition, there may be undetected epistatic interactions with SNPs in other genes across the genome. Strong differences in allele frequencies across race/ethnic groups for such SNPs could modify the observed effect for the ID3 SNP when estimated without taking into account this type of interaction.
The finding that a SNP in a single gene could be associated with a complex polygenic disorder such as atherosclerosis is supported by the fact that Id3 regulates multiple genes within pathways and cell types linked to atherosclerosis. T cells have important roles in atherosclerosis, and Id3 has been implicated in T cell maturation, differentiation [21]–[25], and T cell driven autoimmune disease [26]–[28]. In addition to expression and function within the immune system, the broadly expressed Id3 protein has also been implicated to function in vessel wall cells. In fact, bone marrow transplant studies in mice demonstrate that Id3 mediated atheroprotection is not all due to bone marrow derived cells [29]. 12/15-lipoxygenase, an important enzyme that modifies LDL in the sub-endothelial space of the atheroma, can upregulate Id3 expression in VSMCs and subsequently proliferation of VSMCs [30], [31]. Additionally, VCAM-1, an important adhesion molecule for leukocyte recruitment to the vessel wall, is upregulated in VSMCs and whole aortas isolated from Id3−/− mice [12]. These studies suggest that Id3 has an important role in vessel wall cells to suppress pro-atherogenic processes.
The majority of published data in murine models of atherosclerosis, link Id3 to B cell-mediated atheroprotection. In mice, B cells are present in atherosclerosis prone regions of the aorta, prior to onset of disease. Loss of B cells in these regions is associated with increased atherosclerosis. Compared to control Apoe−/− mice, Id3−/−Apoe−/− mice had a dramatic reduction in aortic B cells and a marked increase in atherosclerosis. Additionally, adoptive transfer of Apoe−/− B cells to B cell deficient μMT Apoe−/− mice attenuated atherosclerosis, an effect that was lost when the Apoe−/− B cells were null for Id3. A role for B cells in human atherosclerosis is supported by the ability of human B cells to produce antibodies specific to modified lipids [32] and demonstrated associations between circulating antibodies to modified lipids and CAD [33], [34]. As B cell-mediated atheroprotection in mice depends on Id3, the association of the functional polymorphism in ID3 with measures of atherosclerosis [11] in humans raises the intriguing hypothesis that ID3 may mediate B cell atheroprotection in humans and the polymorphism at rs11574 may identify those with loss of this humoral immune protection.
The current study does not address these or other mechanisms whereby polymorphism at rs11574 in the human ID3 gene is associated with increased coronary artery plaque volume and calcium accumulation. Yet, taken together, data provide for the generation of novel hypotheses that could be tested in humans and lead to not only novel biomarkers of disease, but novel strategies for disease prevention.
Supporting Information
File S1.
Combined supporting information file including Supplementary Methods and Table S1. Table S1: Characteristics of MESA Whites, African Americans and MESA Hispanics by rs11574 genotype.
The authors thank the participants of the MESA and CAVA studies, the MESA Coordinating Center, MESA and CAVA investigators, and study staff for their valuable contributions. A full list of participating MESA investigators and institutions can be found at http://www.mesa-nhlbi.org.
Author Contributions
Conceived and designed the experiments: SSR CAM AMT. Performed the experiments: AM HP JY ML MD MP MR AMT. Analyzed the data: AM SSR CAM AMT. Contributed reagents/materials/analysis tools: JJC. Wrote the paper: AM SSR HP JY CAM AMT.
- 1. Ragosta M (2011) Techniques for phenotyping coronary artery disease in the cardiac catheterization laboratory for applications in translational research. J Cardiovasc Transl Res 4: 385–392.
- 2. Nicholls SJ, Hsu A, Wolski K, Hu B, Bayturan O, et al. (2010) Intravascular ultrasound-derived measures of coronary atherosclerotic plaque burden and clinical outcome. J Am Coll Cardiol 55: 2399–2407.
- 3. Calvert PA, Obaid DR, O'Sullivan M, Shapiro LM, McNab D, et al. (2011) Association between IVUS findings and adverse outcomes in patients with coronary artery disease: the VIVA (VH-IVUS in Vulnerable Atherosclerosis) Study. JACC Cardiovasc Imaging 4: 894–901.
- 4. Yeboah J, McClelland RL, Polonsky TS, Burke GL, Sibley CT, et al. (2012) Comparison of novel risk markers for improvement in cardiovascular risk assessment in intermediate-risk individuals. JAMA 308: 788–795.
- 5. Campbell KA, Lipinski MJ, Doran AC, Skaflen MD, Fuster V, et al. (2012) Lymphocytes and the Adventitial Immune Response in Atherosclerosis. Circ Res 110: 889–900.
- 6. Lahoute C, Herbin O, Mallat Z, Tedgui A (2011) Adaptive immunity in atherosclerosis: mechanisms and future therapeutic targets. Nat Rev Cardiol 8: 348–358.
- 7. Hansson GK, Hermansson A (2011) The immune system in atherosclerosis. Nat Immunol 12: 204–212.
- 8. Libby P (2002) Inflammation in atherosclerosis. Nature 420: 868–874.
- 9. Libby P, Ridker PM, Hansson GK (2011) Progress and challenges in translating the biology of atherosclerosis. Nature 473: 317–325.
- 10. Lusis AJ, Mar R, Pajukanta P (2004) Genetics of atherosclerosis. Annu Rev Genomics Hum Genet 5: 189–218.
- 11. Doran AC, Lehtinen AB, Meller N, Lipinski MJ, Slayton RP, et al. (2010) Id3 is a novel atheroprotective factor containing a functionally significant single-nucleotide polymorphism associated with intima-media thickness in humans. Circ Res 106: 1303–1311.
- 12. Lipinski MJ, Campbell KA, Duong SQ, Welch TJ, Garmey JC, et al. (2012) Loss of Id3 increases VCAM-1 expression, macrophage accumulation, and atherogenesis in Ldlr−/− mice. Arterioscler Thromb Vasc Biol 32: 2855–2861.
- 13. Perry HM, Oldham SN, Fahl SP, Que X, Gonen A, et al. (2013) Helix-loop-helix factor inhibitor of differentiation 3 regulates interleukin-5 expression and B-1a B cell proliferation. Arterioscler Thromb Vasc Biol 33: 2771–2779.
- 14. Bild DE, Bluemke DA, Burke GL, Detrano R, Diez Roux AV, et al. (2002) Multi-ethnic study of atherosclerosis: objectives and design. Am J Epidemiol 156: 871–881.
- 15. Mintz GS, Nissen SE, Anderson WD, Bailey SR, Erbel R, et al. (2001) American College of Cardiology Clinical Expert Consensus Document on Standards for Acquisition, Measurement and Reporting of Intravascular Ultrasound Studies (IVUS). A report of the American College of Cardiology Task Force on Clinical Expert Consensus Documents. J Am Coll Cardiol 37: 1478–1492.
- 16. Nichaman MZ, Hamilton HB, Kagan A, Grier T, Sacks T, et al. (1975) Epidemiologic studies of coronary heart disease and stroke in Japanese men living in Japan, Hawaii and California: distribution of biochemical risk factors. Am J Epidemiol 102: 491–501.
- 17. Keys A, Menotti A, Karvonen MJ, Aravanis C, Blackburn H, et al. (1986) The diet and 15-year death rate in the seven countries study. Am J Epidemiol 124: 903–915.
- 18. Chan L, Boerwinkle E (1994) Gene-Environment Interactions and Gene Therapy in Atherosclerosis. Cardiology in Review 2: 130–137.
- 19. Welch CL, Bretschger S, Latib N, Bezouevski M, Guo Y, et al. (2001) Localization of atherosclerosis susceptibility loci to chromosomes 4 and 6 using the Ldlr knockout mouse model. Proceedings of the National Academy of Sciences of the United States of America 98: 7946–7951.
- 20. Wang Q, Rao S, Shen GQ, Li L, Moliterno DJ, et al. (2004) Premature myocardial infarction novel susceptibility locus on chromosome 1P34–36 identified by genomewide linkage analysis. American journal of human genetics 74: 262–271.
- 21. Jones-Mason ME, Zhao X, Kappes D, Lasorella A, Iavarone A, et al. (2012) E protein transcription factors are required for the development of CD4(+) lineage T cells. Immunity 36: 348–361.
- 22. Maruyama T, Li J, Vaque JP, Konkel JE, Wang W, et al. (2011) Control of the differentiation of regulatory T cells and T(H)17 cells by the DNA-binding inhibitor Id3. Nat Immunol 12: 86–95.
- 23. Miyazaki M, Rivera RR, Miyazaki K, Lin YC, Agata Y, et al. (2011) The opposing roles of the transcription factor E2A and its antagonist Id3 that orchestrate and enforce the naive fate of T cells. Nat Immunol 12: 992–1001.
- 24. Ji Y, Pos Z, Rao M, Klebanoff CA, Yu Z, et al. (2011) Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits the formation of memory CD8+ T cells. Nat Immunol 12: 1230–1237.
- 25. Yang CY, Best JA, Knell J, Yang E, Sheridan AD, et al. (2011) The transcriptional regulators Id2 and Id3 control the formation of distinct memory CD8+ T cell subsets. Nat Immunol 12: 1221–1229.
- 26. Guo Z, Li H, Han M, Xu T, Wu X, et al. (2011) Modeling Sjogren's syndrome with Id3 conditional knockout mice. Immunol Lett 135: 34–42.
- 27. Li H, Dai M, Zhuang Y (2004) A T cell intrinsic role of Id3 in a mouse model for primary Sjogren's syndrome. Immunity 21: 551–560.
- 28. Mahlios J, Zhuang Y (2011) Contribution of IL-13 to early exocrinopathy in Id3−/− mice. Mol Immunol 49: 227–233.
- 29. Doran AC, Lipinski MJ, Oldham SN, Garmey JC, Campbell KA, et al. (2012) B-cell aortic homing and atheroprotection depend on Id3. Circ Res 110: e1–12.
- 30. Taylor AM, Hanchett R, Natarajan R, Hedrick CC, Forrest S, et al. (2005) The effects of leukocyte-type 12/15-lipoxygenase on Id3-mediated vascular smooth muscle cell growth. Arterioscler Thromb Vasc Biol 25: 2069–2074.
- 31. Deliri H, Meller N, Kadakkal A, Malhotra R, Brewster J, et al. (2011) Increased 12/15-lipoxygenase enhances cell growth, fibronectin deposition, and neointimal formation in response to carotid injury. Arterioscler Thromb Vasc Biol 31: 110–116.
- 32. Chou MY, Fogelstrand L, Hartvigsen K, Hansen LF, Woelkers D, et al. (2009) Oxidation-specific epitopes are dominant targets of innate natural antibodies in mice and humans. J Clin Invest 119: 1335–1349.
- 33. Tsimikas S, Brilakis ES, Lennon RJ, Miller ER, Witztum JL, et al. (2007) Relationship of IgG and IgM autoantibodies to oxidized low density lipoprotein with coronary artery disease and cardiovascular events. J Lipid Res 48: 425–433.
- 34. Morris-Rosenfeld S, Lipinski MJ, McNamara CA (2013) Understanding the role of B cells in atherosclerosis: potential clinical implications. Expert Rev Clin Immunol


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Raging Storm(2017)
(The second book in the Remnant series)
A novel by Vannetta Chapman
When a massive solar flare wiped out all modern technology across the globe, the small town of Abney, Texas, was thrown into chaos. Shelby Sparks and her teenage son, Carter, have found refuge, but Shelby knows she can t simply remain hidden. Carter is a type 1 diabetic in need of insulin and Shelby will risk her life traveling to Austin to make sure he gets it."
Genre: Inspirational
Genre: Inspirational
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Japan's Underwater Ruins
Off the coast of Japan's Yonaguni island are mysterious rock formations that have led to intense debate among archaeologists and geologists. These large stone structures appear to be large, stepped monoliths. Some of the ruins have walls that are 33 feet tall and columns that rise to within 8 feet of the surface. There are square shapes and formations that look like figures, such as the turtle and the giant face have convinced some that the formations are man-made.
The argument that the structures are man-made comes from the presence of right angles as part of the structure and the twin megaliths that appear to have been placed there. Maasaki Kimura, who first discovered the site, says that he had found traces of animal drawings and people in the rocks and a symbol that he believes to be a character from the Kaida script.
Kimura claims that he can identify castles, roads, monuments, and even a stadium in the rock formations. If true this would be astonishing as some date the ruins back 10,000 years. Others present more conservative estimates of 2,000 to 3,000 years, which would still be an astonishing find and would lead to questions about who could have constructed the ruins. Kimura theorized that the ruins could be part of the mythical lost continent of Mu.
On the other side of the debate are geologists who claim that all of the formations are naturally occurring. Yonaguni is found in an earthquake prone region and earthquakes have been known to cause sandstone to fracture in shapes similar to those found at the ruins. They believe the roads are just channels in the rock and the vertical formations are just rocks that were horizontal but fell vertical when the rocks below them eroded. Others say it is unusual to see so many of these types of formations in such a small area but there is no definitive evidence that concludes the formations at Yonaguni are indeed man-made.


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So I basically cut three of the shelves to the same length and used the saw table to rip down the edges of two of the boards so that the finished sign was 20" wide. I cut three boards for the back then went to town sanding off the old paint with an orbital sander. I sanded it almost all the way but loved the dark lines that were left.
Then I toasted "toasted" with two shades of a cream colored paint and some brown paint. After the paint dried I covered the front, edges, and back with about 6 coats of a matte finish Rustoleum sealer.
It's not perfect. The spacing on some of the words could have been closer and there were some spots where the paint bled under the stencil, but I love those imperfections. They show the love that went into making this lovely sign.
The finished sign is about 20" x 30". The best part about this project? It cost a whopping $4.00 for a can of spray sealer. Everything else I already had at home. Not to mention it recycled an old shelf that was just headed for the landfill. And I still have a couple beautifully sanded boards for some other project in the future. I'm thinking maybe a Corbet Family Est. 2005 sign.
Now Kelly and Eric just have to figure out how they want to hang it!
Any ideas on what I can make with the two 2 x 8 boards I have left? They are about 4-1/2 feet long. I'm thinking maybe a bench for outside? Any thoughts?
Updated to add:
A smaller version of this sign is for sale in my Etsy shop! Just click HERE to view all my hand painted signs. Looking for a custom size? Just send me a message via Etsy or E-mail and I build it as large as you like. Just be advised that pricing and shipping costs are more with a larger sign.
Linking up here:
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Show Me What Ya Got at Not Just a Housewife


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A Visit to Kmart + Barbie Ban
Did I mention I took my 'Barbie Budget' down from $200 to $0? Yup, $0 a year. It's unlikely to happen, but I want to be as close to $0 as possible. Presents don't count.* *Not that anybody will be giving me any Barbie presents, since the only Barbie "presents" I get are presents to… Continue reading A Visit to Kmart + Barbie Ban


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Ascending Mount Carmel: Why St. Rose of Viterbo Matters So Much Right Now….
Thank you. I had never heard of St. Rose of Viterbo. I wonder how she would have acted if she lived in our time and her religious freedom, freedom of conscience and religious beliefs were under attack. I think I know the answer, but I also wonder how many of us would stand with her.
A new commandment I give unto you, That ye love one another; as I have loved you ... John 13:34
"You will be my witnesses." Acts 1:8


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The Navy vs. the Night Monsters is a 1966 American science fiction film directed by Michael A. Hoey. The movie was based on the novel The Monster from Earth's End by Murray Leinster.Operation Deep Freeze, a scientific expedition to Antarctica discovers unusual tree specimens. When specimens are shipped out for further study, the trees are accidentally introduced to a south seas Navy base, soon revealing themselves to be killer, acid-secreting monsters that live by night.
Our postcards are professionally printed on glossy card stock. The reverse side has a matte finish to ensure that the ink while writing adheres properly. Each card measures 6 by 4 inches and matches the post office standard for postcard mailing. Just add a stamp and address and you are ready to send someone a truly unique postcard!


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NGC 2264 the Christmas Tree cluster and cone nebula are designated under the same number located in Monoceros constellation. You can clearly see the shape of a Christmas tree in the cluster. There are a few more nebula associated with 2264 the snowflake and Fox Fur nebula.
In this photo you can make out the snowflake and cone. With a modified camera or HA filter the nebula would be more prominent. Shot with Nikond3200 at ISO 1600. Celestron Nexstar 8 and 74/25second subs. About 32 minutes exposure.
[Website created in the IYA-2009: International Year of Astronomy]


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Lotus Flower Keepsake Bracelet
Limited edition from our Keepsake family is our Lotus Flower charm available in gold, silver or rose gold with a selection of different colour stones to personalise your Keepsake bracelet.
The lotus flower is a symbol used in many faiths and has beautiful symbolic and association meanings including purity, spiritual awakening and faithfulness as well as a sign of re-birth.
Bracelet shown in image is in amethyst with a rose gold vermeil lotus flower pendant.
Bracelet measures 18cm long but can me made to order to any size requested.


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Active @properties exclusive
23475 State Road 4
Lakeville, Indiana 46536
Lori M. Osmanski312.391.4870
Half Baths1
Magnificent Country Estate on four acres is the perfect blend of refined, country living. It's majestic surrounding and sprawling 8000 sq. ft. home has all the modern amenities life has to offer. The main house has 11 foot ceilings, open floor plan, four bedrooms, three and half baths, gourmet kitchen, Master suite, bath and walk in closet, finished basement, bar, exercise room, massage and tanning room, home theater and a custom in door pool with direct excess to a open patio. The second 5000 ft building has a two bedroom in-law apartment with a massive garage attached for storing a boat, or car collection, it has 2,fourteen ft and 3,ten ft. overhead doors, perfect for a business. This fabulous home is only 10 min. from South Bend and 1 1/2 hours from downtown Chicago. A perfect full time, or weekend retreat home. This home is in pristine condition and move in ready, it is priced to sell, do not miss out on this opportunity.
Full Features
Architecture Style: Other
Exterior Type: Brick,Vinyl
Beds Above Grade: 4
Heating System: Propane Gas, Forced Air
Appliances: Dishwasher -Built-In, Disposal, Double Oven-Built In, Dryer, Exhaust (Kitchen), Freezer, Gas Range -Built In, Microwave, Range Hood, Refrigerator, Washer
First Floor Master Bedroom: Yes
Fireplace: Yes
Pool: Yes
Parking Type: - Attached,Garage Door Opener,Other
Parking Spaces: 3
Room Count: 14
Square Feet: 8,000
Tax Amount: $6,678
Tax Year: 2015
External Features: Covered Porch, Enclosed Porch, Hot Tub, In Ground Pool, In Ground Sprinklers, Landscape Lighting, Patio, Pool House, Screen Porch, Other
Room Information
Master Bedroom: 22x16
2nd Bedroom: 12x12
3rd Bedroom: 12x12
4th Bedroom: 12x14
Living Room: 19x22
Dining Room: 12x15
Kitchen: 16x14
Family Room: 34x19
Other: 12x13
Enclosed Porch: 22x18
Other: 23x15
Taxes & Assessments
Taxes: $6,678
Listing information updated today (08/19/2017)
School District(s)
Union-north United School Corp
Elementary School
Middle School
High School
School Assignment Data provided by the National Center for Education Statistics
Nearby Public Transportation
No Nearby Public Transportation Found


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| This article has been evaluated to be at quality level 5.
This article may seem complete to the untrained eye, but the gaps in information are still large enough for this elite team of Panauan Ninjas to hide inside of.
|Bukit Tinggi
|Settlement in Panau
|Military base
|Berawan Besar Mountains
|Missions that take place here
Bukit Tinggi is a military base in Just Cause 2.
It's a communications base, similar to many other bases in the district.
The base consists of a lot of bunker buildings, some hangars and other generic equipment inside of its walls. As usual, there are radars in three of the corners. There are also some guard towers in the area. A little way from the main base is a communications outpost. The access way entrance is guarded with two miniguns. There's also a helicopter landing pad at the outpost, but no helicopter on it. There are several high cliffs below the outpost.
A notable thing is that the communications mast is located just outside the base walls, instead of inside. There's also a lone bunker building outside the base walls.
The name means "High Hill" in Malay, which makes sense considering its location: high in the Berawan Besar Mountains, west of the Snow Peak.
A long restricted road leads up to this base from a public road.
- 3 Broadcast Towers.
- 3 Generators.
- 3 Radars.
- 1 Communications Outpost.
- 1 Communications Mast.
- 12 Resource items:
- 6 Weapon parts.
- 4 Vehicle parts.
- 1 Cash stash.
- 1 Armor part.
- SV-1003 Raider.
- AH-33 Topachula - This is not on a helipad as usual, but on the ground inside the base.
- GV-104 Razorback.
- Fengding EC14FD2.
- Maddox FVA 45.
- There are cities in Malaysia and Indonesia that have the same name. List at wikipedia.


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Simply NUC NUC 7 I3 4GB 128GB UBUNTU (910-8C16-031)Item#: 40613991 | Model#: 910-8C16-031
- Price:
- $541.99
$6.99 Shipping
- Shipping:
- Temporarily out of stock. Order today and we'll deliver when available. (Details)
Additional Resources
Simply NUC NUC 7 I3 4GB 128GB UBUNTU Product Details
Additional Resources: Product Manuals
Recommended ItemsPrevious
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Manufactured by: Simply NUC
Warranty provided by: Simply NUC
UPC No:
Mfg Part No: 910-8C16-031
Box Size:
( Length:0, Width:0, Depth:0)
Shipping Weight: 2.5400 pound(s)
Click here for full warranty and support information
- Simply NUC logos are registered trademarks of Simply NUC. All others trademarks and copyrights mentioned herein are the property of their respective owners.


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Our Menu
Everything we have to offer at one glance
Our philosophy is to take bits of the old traditional Italian cooking styles and assemble them in new ways using the freshest seasonal ingredients. We hand craft our own Pasta & Gnocchi and our Pizza's are straight from our wood fired oven. – It's authentic Italian created in the heart of Wellington.


text: This article is a disambiguation page for Achievement The following is a list of links to pages that might share the same title. Please follow one of the disambiguation links below or search to find the page you were looking for if it is not listed. If an internal link led you here, you may wish to change the link to point directly to the intended article. Platinum Blades and Dark Corners. Achievement may refer to the following: Dishonored Achievements Dunwall City Trials Achievements The Knife of Dunwall Achievements The Brigmore Witches Achievements Dishonored 2 Achievements Retrieved from "http://dishonored.wikia.com/wiki/Achievement?oldid=150450" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Disambiguation pages The Knife of Dunwall Achievements The Brigmore Witches Achievements Dishonored 2 Achievements Dishonored Achievements Dunwall City Trials Achievements


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Discontinued - Atomos Samurai HD-SDI Hard Disk Recorder
- Condition:
- New
Please see updated model Atomos Samurai Blade
Atomos Samurai HD-SDI Hard Disk Recorder captures 10-bit HD resolution video directly from you HD camera and stores it to an internal 2.5" laptop drive. Images are captured using Apple's lossless ProRes codec and are ready to edit on Final Cut Pro or on a Windows machine equipped with the ProRes codec and a supporting NLE editor. Along with capturing video, the Samurai supports 8 channels of embedded SDI audio and also has 2 channel line in inputs as well as a single headphone output.
A high resolution 5" on-board monitor provides you with an accurate representation of what the camera is seeing, eliminating the need to mount an extra monitor. Touch screen controls - presented with large icons on an uncluttered interface - give you easy access to record and playback functionality as well as to the system's menu. Some of the advantages the Samurai has over the Ninja unit are timecode, genlock, and the ability to synchronize multiple units.
Samurai makes it easy to go from the capturing to the editing process. Once capturing is complete, simply remove the 2.5" SSD or HDD with its caddy from the recorder and place it in the included docking station which interfaces to the host PC via FireWire 800 or USB 3.0. A LANC connector lets you integrate the Samurai with cameras and devices that utilize the LANC communication protocol.
Samurai comes with 2 batteries that securely attach to the unit, but uses only one at a time. This way if one battery runs out, Samurai continues to operate on the other battery.
- Post Production Quality, 10-Bit Recording
- Samurai takes the uncompressed video output from your camera and, in hardware, encodes it in real-time to visually-lossless Apple ProRes, recognized across the industry as a class-leading codec for editing and post-production. Record direct from your camera's sensor electronics direct into super high quality Apple ProRes for the most streamlined, affordable Final Cut Pro workflow available today. 1
- A Pipeline to Your Timeline
- File-based production is all about efficient workflow, and Samurai takes the workflow to the camera - not the other way round. The Samurai's tight Apple ProRes integration means recorded footage is instantly and natively editable in Final Cut Pro. No need to waste time transcoding or converting from other camera formats. And remember - Apple ProRes is a better editing codec than long-GOP delivery or camera codecs like AVCHD or similar MPEG flavors. Samurai uses standard computer connections - FireWire 800 and USB 2 and 3 to give you total flexibility when connecting to your Mac or PC. Simply insert your Master Caddy into the Master Caddy Dock provided and you are editing instantly. You can also, of course, transfer the footage to your central SAN or LAN.
- Incredibly Low-Cost High-Capacity Storage
- Flash memory is fine for stills photography, but with High-Definition video, quality is compromised by the extreme compression needed to fit huge quantities of data onto tiny in-camera storage media. Professional storage media like SxS and P2 are simply too expensive to "use and forget", which means that you're never free to just work as you want to. Samurai liberates you from the financial and operational constraints of expensive Flash media by allowing you to use cheap, commodity storage available in any computer store: massive yet inexpensive traditional 2.5" Hard Disk Drives (Spinning Disks) for most projects and Flash- based SSDs for rough-and-tumble use. (Buying Flash memory in the form of SSDs is much cheaper than the equivalent memory size on in-camera flash memory cards). Using tried and tested storage guarantees performance and reliability. A 750GB/5400rpm drive holds up to 16 hours of high quality Apple ProRes footage and costs under $100.
- Recycle Older Cameras
- Samurai records direct from the HD-SDI output from your camera, so it doesn't care what format your camera is: whatever it receives through the HD-SDI connection it will record (with certain exceptions detailed on www.atomos.com). So if you've got an older camera with HD-SDI - just plug in a Samurai and bring it into the world of file-based production. You'll get better quality from your old camera than when it was new!
- Simple-to-use 5.0" Touchscreen
- Access all of the Samurai's operations via the fast, responsive touch interface. New features are easy to add with software upgrades. All the main functions are accessed through big, colorful icons. It's so simple, there's no need for complicated menus
- Continuous Power from Hot-Swappable Batteries
- Powering video devices on location is no longer a worry because the Samurai comes with 2 batteries, each capable of a minimum of 2.5 hours, and a combined maximum of up to 9 hours continuous operation when fully charged, depending on codec and screen use. Samurai has two battery compartments but only uses one at a time and switches to the second battery when the first is depleted. Simply swap batteries on the fly without stopping the production!
- Latching Batteries Safe and Secure
- Batteries are held in place on the outside of the Samurai. To ensure that they never come lose, Atomos has designed an ingenious latching system that holds batteries in place securely, but releases them instantly if you release the catch.
- Everything You Need
- Samurai works right out of the box - you can be using it within minutes! Samurai is supplied with everything (except storage media which is cheaper to source locally) in a tough professional carry-case. The Samurai, two Master Caddies ready to receive your storage media, a USB 2 + 3 + FireWire 800 docking station, two 2600mAH batteries and a smart battery charger, together with cables for connecting all of this together. There's no need for software drivers and no installation is necessary.
Operating Power Approx 6.8W (TFT max brightness, recording Apple ProRes HQ to Hitachi 7200 rpm 500G drive) / approx 3.3W (TFT max brightness, monitoring only) Batteries 2 x Small 7.4V 2600mAh - recording time
5 hrs HQ; 7 hrs 422; 9 hrs LT
2 x Medium 7.4V 5200mAh - recording time
10 hrs HQ; 14 hrs 422; 18 hrs LT
2 x Large 7.4V 7800mAh - recording time
15 hrs HQ; 21 hrs 422; 27 hrs LT
Recording time based on 1080i60 extreme detail video; better at lower rates or with typical video Compatible with up to 14.4V battery systems via adapter cable. Automatic and manual switchover of battery for continuous operation
Screen Color 16:9 TFT Touch Screen Screen Size 5" / 12.7 cm (diagonal) Resolution 800 x 480 Aspect Ratio 16:9 Native / 4:3 Letterbox (screen flipping enabled) Video Input DI SMPTE 259/292/296 Mini BNC (with BNC Adaptor cables) Video Output SDI Loop out Input Formats HD 1080i60, 1080i59.94, 1080i50, 1080p30, 1080p25, 1080p24, 1080p23.98, 720p60, 720p59.94, 720p50, SD 480i, 576i Video Processing 3:2 Pull-down removal. Double anti-shock buffer Recording Time ProResHQ - 3 hours (HDD 300 GB), 5 hours (HDD 500 GB), 7.5 hours
(HDD 750 GB), 77 min (SSD 128 GB), 2.5 hours (SSD 256 GB)
ProRes422 - 4.5 hours (HDD 300 GB), 7.5 hours (HDD 500 GB), 11.5 hours
(HDD 750 GB), 2 hours (SSD 128 GB), 4 hours (SSD 256 GB)
ProResLT - 6.5 hours (HDD 300 GB), 11 hours (HDD 500 GB), 16.5 hours
(HDD 750 GB), 3 hours (SSD 128 GB), 4.5 hours (SSD 256 GB)
All video input formats supported
Audio Input SDI up to 8 Channel Embedded
Line In 2 Channel Line Level Audio. Analog gain adjustment
Audio Output Channel 0.5W Balanced Headphone or Line level output. LANC LANC In and Out Loop for integration with Camera LANC and Accessories
LANC Loop feature
Dimensions (WxHxD) 5.5 x 3.43 x 1.61" (140 x 87 x 41 mm) - without batteries Master Caddy Storage 2.5" SSD or HDD minimum 5400 rpm
Standard 9.5 mm height or slimmer drive supported
Master Caddy Dock 2.5" SATA to Firewire 800, USB 2.0 / 3.0 Supported Computers Mac - All MacBook, MacPro, iMac and MacPro Models
Windows All USB2.0/3.0 compatible Notebooks, and Desktops
Applications Final Cut Studio - All Apple ProRes supported versions Quicktime Mac and Windows Dimensions 2.95 x 4.13 x 0.47" (75 x 105 x 12 mm)
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text: Artist Application Download Performer Guidelines for 2018 here! Complete the form below to apply for the Asheville Fringe Arts Festival. Name of Artist Company Name Address (include country) Contact Information Contact Name Contact E-mail Daytime Telephone Number Evening Telephone Number Which terms would you use to describe your piece? TheaterDance/MovementMusicVideo/FilmPerformance ArtPoetryPuppetryInstallation with PerformanceFamily-FriendlyAdult OnlyOther (add comments below) About Your Submission Title of Piece Description of Piece-Please fully describe your piece and include a link to video of the performance or your website Is this submission for a Full Length Piece (45-60 minutes long. See guidelines) YesNo What is the total length, in time, of your submission? How many performers are involved in your submission? What are your technical needs? If yours is not listed below, be certain to add it to the comments section. Remember that Artists are required to bring their own technical equipment. NoneWindows-based PCApple PCProjectorSound equipmentOther Comments: The Festival is a four day event. Please indicate all of the days you will be able to perform: Thursday, January 25Friday, January 26Saturday, January 27Sunday, January 28 Are you willing and able to adjust your piece to a non-standard performance space? (e.g. a space that is not a black box theatre space, limited lighting and tech available) YesNo Is your piece intended for general audiences, or a more mature audience (sexual content, nudity, violence)? Please describe: If accepted, and you are traveling from out of town, do you require housing? YesNo If yes, then what are your specific housing needs? (Number of people, number of rooms, special considerations needed) I have read the Asheville Fringe Arts Festival Guidelines and understand the expectations of me and my group, as well as what I can expect from the festival staff. I will make every effort to attend the adjudication committee meeting or provide the committee with information, including video links, prior to decision making process.