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My smackdown of George Weigel has given birth to a very interesting and important exchange between two professors I greatly admire, Notre Dame's Rick Garnett and Fordham's Charles Camosy. At issue is the relationship between agreed-upon Catholic doctrine and the exercise of prudential judgment by politicians. This is a critical discussion and I hope that all three of us can help perfect the arguments of each of us.
We say: Charlottesville reveals the weeping wound of racism. What do we, the American Catholic faith community, do next? Read the editorial.
Both Garnett and Camosy are right, but how can that be? And both men are wrong too.
They have each updated their discussion as of this morning. Garnett's update is at the end of the item linked above and Camosy's uldate is at the end of his post linked to above.
Garnett is right to argue that our current abortion laws are "fundamentally unjust" and the adverb is important. The problem with our nation's abortion laws is not only that they conflict with Catholic doctrine, but that they make a mockery of the exalted ideas of the rights of the human person which serve as the foundation of our democracy. Our current abortion laws say that some people can be excluded from legal protection, provided those people are very tiny and still largely reliant upon their mother to continue living. Of course, an infant is still very tiny and largely reliant upon its mother too. So, our abortion laws attack not only our sense of justice, but betray the opening three words of the Constitution, "We the People," by saying some people are not included.
Camosy is right, however, to insist that recognizing the almost uniquely regrettable moral failings that our nation's abortion laws represent does not tell us very much about how to redress those laws. Part of politics is calculating the strength of the opposition and anticipating blowback. Americans are largely ambivalent about abortion. They don't much like it, but they are skittish about denying a woman access to it. Why? Because some are old enough to remember the days of back alley abortions, and others can more easily empathize with the circumstance of a young woman who finds herself unexpectedly pregnant more than they can empathize with an unborn, unseen child. The endurance of the Hyde Amendment, year after year, despite changes in almost every other aspect of national policy, testifies to the persistence of discomfort, the willingness to recognize that abortion is not like other issues. The concern to which I think Charlie is pointing is this: Is there really any doubt that if Roe v. Wade were overturned tomorrow, within a matter of weeks, most states would adopt statutes that continue the broad outlines of Roe? Say, of the sake of argument, most states did not do so, that they criminalized elective abortion. Would that criminalization survive long if even a few women were to die from obtaining illegal abortions?
In short, I don't think Garnett and Camosy are really arguing about the relative moral weight of abortion versus, say, tax policy. I think they are arguing about how to change our democracy. If we in the pro-life movement do not do a better job convincing our fellow Americans to see the issue as we do, we will fail to achieve what we seek, legal protection for all people including the unborn. I would add that as long as some of the more prominent leaders in the pro-life movement are attacking CCHD and CRS, our moral witness is profoundly compromised. I would add, too, that this difficulty in persuading our fellow citizens flows from the same Enlightenment premises, in this case that government is legitimate only when it rests on the consent of the governed, that have tied our nation up in knots over the HHS mandate. In the latter case, no matter how many times we point out that religious freedom is guaranteed by the First Amendment, there is no escaping the fact that many of the founders understood religion, conscience and rights differently from the way we Catholics understand them.
A final thought. I do not want to let tax policy, and economic policies more generally, off the hook by suggesting it falls so far into the category of "prudential judgment" that we conclude that Catholics can essentially think whatever they want on these subjects. My most basic criticism of Garnett, and Weigel, is that they are too quick to accept as a "given" modern ideas about the economy. Surely one of the benefits of being a Catholic is the ability to take the long view, to recognize that there were many centuries of Christian witness before the advent of modern capitalism and there will someday be many centuries of Christian witness after the demise of modern capitalism. But, while we are living in this capitalistic age, we must be just as critical of its anti-Christian precepts as we are critical of the idea that a woman's choice trumps the right to life of her unborn child. Here I would turn to Gaudium et Spes:
Here is the consistent ethic of life. Gaudium et Spes does not say that abortion and disgraceful working conditions are the same thing but it does insist that both violate our core Catholic beliefs about human dignity. Enlightenment ideas that exalt human choice may be responsible for our nation's mistaken laws on abortion and other Enlightenment ideas that exalt human choice have given us an economic system that, quite obviously, fails to hear the cries of the poor. And, make no mistake about it - while abortion may present a more foundational and fundamental threat to human dignity, bad economic policy is a more pervasive threat. The loss of a human life may be more morally foundational than an unjust economic policy, but Americans and American culture are more determined by people's self-awareness as homo economicus than it is as potential parents.
Any Catholic in America today who takes Gaudium et Spes seriously must understand themselves to be politically homeless. The debate between Garnett and Camosy is useful, and it is edifying insofar as it is being conducted with such civility and with both intellectual integrity and intellectual heft. But, I hope the disagreement leads not only to a meeting of the minds, but a meeting of the wills, and that both men may become part of an urgent discussion all politically engaged Catholics need to have. If we are politically homeless, perhaps it is time we create our own home.
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Senior Finance Business Partner
- Competitive
- Londres, Angleterre, Royaume-Uni
- CDI, Plein-temps
- Transport for London
- 14 août 17
Millions of people rely on our network. For business. For leisure. For living. Keeping London's growing population on the move takes billions of pounds - and a wealth of expertise, like yours.
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As a Senior Finance Business Partner, you'll play a pivotal role in challenging perceptions about public sector finance and the role it can play. You'll also bring the courage to challenge the business about how we are performing.
A true business partner, you'll manage a customer focused finance team dedicated to driving TfL to achieve strategic goals. It's about making sure every decision is well planned and executed, minimising risk and using resources in the most effective way.
You may find yourself partnering any one of a range of diverse teams. These might be from our Major Capital Projects directorate and could include London Underground Major Capital Projects; Surface Transport Major Capital Projects; Major Projects Directorate Planning & Reporting; Operations; Assets and Engineering; or Renewals and Enhancements Capital Projects. Alternatively, you may partner with a Professional Services team such as Technology Projects (including Oyster card) or Customer & Stakeholder.
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Say hello to the leakproof, tough-as-nails, carry-the-day soft cooler. Around here, we call it the Hopper Flip™ 12. Like the original Hopper™, the Hopper Flip... View full product details
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Are you looking for a fun-filled and action-packed team building experience in Lancaster County? Landing Zone takes laser tag to new heights! Our state-of-the-art equipment and immersive battle arena provides a unique platform for team building, adventure and exercise!
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6009 Chapel Drive
Edina, MN 55439
$450,000 (Pending)
Beds: 4
Baths: 1 | 1
Sq. Ft.: 2,315
Perfectly arranged floor plan for comfortable living - You take up, with office and extra bedroom - a great 4th bedroom for a private teen or guest area. Upgrades and care show up throughout entire home from hardwood floors to new bath. Backyard with gardens, patio and fire pit signals big or cozy parties.
Property Features
Location Information
County: Hennepin
Map Code: 133D1
Latitude: 44.879172
Longitude: -93.376015
Directions: South of 62 on Tracy, then Left on Antrim, then Right on Chapel Drive.
Interior Features
Extra Room Type: Office
3/4 Baths: 1
Other Bathrooms Description: Main Floor 3/4 Bath, Upper Level Full Bath
Has Dining Room: yes
Dining Room Description: Separate/Formal Dining Room
Has a Family Room: yes
Family Room Description: Main Level, Lower Level
Has Fireplace: yes
Number of Fireplaces: 1
Fireplace Description: Living Room
Heating: Forced Air
Heating Fuel: Natural Gas
Cooling: Central
Disabled Features: None
Appliances: Range, Microwave, Dishwasher
Basement Description: Full, Finished (Livable), Drain Tiled, Sump Pump
Has Basement: yes
Exterior Features
Construction: Previously Owned
Exterior: Fiber Board
Roof: Asphalt Shingles, Age 8 Years or Less
Septic Sewer: City Sewer/Connected
Water: City Water/Connected
Parking Description: Attached Garage, Driveway - Asphalt, Garage Door Opener
Has Garage: yes
Number of Garage Spaces: 2
Fencing: Partial
Lot Description: Tree Coverage - Light
Lot Size (Acres): 0.275
Zoning: Residential-Single
Road Frontage: City
Has WaterFront: No
School District: Edina
Additional Information
Property Type: SFR
Property SubType: Three Level Split
Year Built: 1959
Status: Pending
Is a Foreclosure: No
Lender Owned: No
Potential Short Sale: No
Unit Features: Patio, Natural Woodwork, Hardwood Floors, Washer/Dryer Hookup
HOA Fee: 0
Extra Room2 Type: Patio
Room Dimensions (LxW): BR1: 12x10, BR2: 13x12, BR3: 13x11, BR4: 13x11, DR: 11x9, KT: 15x11, LR: 20x12, FR: 20x13
Tax Year: 2016
Tax Amount: 5, 092
$ per month
Year Fixed. % Interest Rate.
|Principal + Interest:
|Monthly Tax:
|Monthly Insurance:
Based on a fully amortized fixed rate loan. Ask your agent for the tax rates in your area. Insurance estimate is based on an average cost, your final premium cost will be determined by the type of coverage you select. This program only provides an estimate.
Seller's Representative:
Edina Realty, Inc.
Edina Realty, Inc.
Lakes Sotheby's International Realty is not a Multiple Listing Service (MLS), nor does it offer MLS access. This website is a service of Lakes Sotheby's International Realty, a broker Participant of the Regional Multiple Listing Service of Minnesota, Inc. Information Deemed Reliable But Not Guaranteed.
© 2017 Regional Multiple Listing Service of Minnesota, Inc. All rights reserved.
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NorthstarMLS® data last updated at August 18, 2017 3:15 AM CT
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We are in the month of new month March and im up with the review of cheap or free Internet data connection in the country which involves the whole net in Nigeria mtn, glo, etisalat and airtel.
Cheap or free mtn, etisalat, airtel and Glo Internet data for March 2016
MTNMtn have been really good to us since the turn of this year, you can browse free without any charge or little amount and download Unlimited and stream as well. People kept on saying that the mtn N0.0k isn't working for them but yesterday i bought a new sim and tried it especially with music plus settings and it connected though takes time.
Mtn BB10 Bis; This is almost going to 2-3yrs now this was introduced to Android and mtn still allowed it to work for Android, iPhone and PC, though they blocked BBC which is Unlimited and that was what everyone was using, they allowed bb10 to continue working on other platform not just bb10 phone.
STill now mtn BB10 Bis is still working for both Android, iPhone and PC with data still capped at 5GB.
Mtn Magic Sim, N0.0k, music plus and GM 150mb; in this category, mtn Magic Sim is the best as I'm using it, will cost you just N250 to do it, browse, download and stream unlimited without any data and to crown it all it works on any type of operating system Android, iPhone, Windows mobile, PC, MAC, Blackberry etc as it doesn't require any tunnel software.
Mtn N0.0k is still working for some according to a reader here and said that he is using "musicplus.mtnonline.com/data-reset" in proxy server that is the settings that i posted in my previous post.
Mtn music plus and GM are working but capped at 150mb but you can't continously getting the 150mb per day.
EtisalatIs quite unfortunate that we are left with etisalat smart pack as etisalat magic ips was blocked few weeks back, nothing much with etisalat other than social and chat pack which are now extremely difficult to bypass the speed throttling. I no longer fancy think tweak again as etisalat made it difficult for one to enjoy it like before. But etisalat have 1GB data capped at N1000 and 2GB at N2000, if you need more information go to their Website www.etisalat.com.ng or mobile.etisalat.com
AirtelFor now nothing much on airtel free browsing though I'mtrying to figure out something and hopes it works out but for the main time use airtel data bundle. They have 2GB for N1500, airtel weekend data and time based but man i will recommend you to use mtn as it is the cheapest you can get for now.
GloEven though Glo increased their monthly blackberry data bundle plan, you can go for Glo 6GB for N1500(Now N1900) which is the combination of two data bundle and here's the procedure to activate Glo N1500 but now N1900 as GLO increased the blackberry monthly data plan. If you noticed immediately some fools posted this on nairaland, glo increased their monthly blackberry data plan from N1000 to N1400.
How to activate Glo N1500 for 6GB (Now N1900) data plan
1 Load N1,000 airtime and activate the monthly plan of 3GB by dialing *777*21# and you'll be given 3GB for 30days.
2. Also, load N500 airtime on that same Glo Sim card and dial *777# and follow the prompt [Data Services » Buy Data » Night and Weekend plans], then select 2 for weekend plan to activate the Glo 3GB for N500.
After that switch off your phone and power it on again and you are good to go.
These are the cheapest data bundle in Nigeria for March 2016 zpykios blog would recommend to anyone, but if you have any you want to suggest no problem just feel free and drop your opinion thanks.


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VISE Oval & Power Lift Blend White
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TUSCALOOSA | The olive branch has been broken.
After agreeing in April to drop a potential lawsuit and stop speaking out against the city of Tuscaloosa over its sewage spills and their effects on area streams, John Wathen, director of Friends of Hurricane Creek, directed an emotional tirade at city leaders Thursday night over the July 1 spill near Cumberland Road.
"My right to use my land has been stolen from me .," said Wathen, who lives on Hurricane Creek. "The creek stinks, and it's full of dead fish."
In March, the Friends of Hurricane Creek filed an intent to sue notice against the city if area sewage leaks were ignored.
Six weeks later, Wathen agreed, at the behest of Rep. Artur Davis, D-Birmingham, to drop the threat of legal action on the condition that the city act in "good faith" to prevent future spills.
"This is not happening," Wathen said Thursday night.
Wathen was further angered when City Council President Jerry Plott cut him off after exceeding the City/sCouncil's five-minute limit on public comments.
"We can't resolve whatever problems you've got here," Plott said and suggested Wathen take his concerns to the mayor's office. Mayor Al DuPont was not at Thursday's City Council meeting.
"For them to take me off the floor . is deplorable," Wathen said after the meeting. "This is not the way democracy works."
He said the City Council has not heard the last of his complaints, and that he and the Friends of Hurricane Creek board of directors are considering pursuing litigation once again.
Before the City Council meeting, Tuscaloosa Water and Sewer Director Maurice Sledge said that last week's malfunction of a city sewage pump station has prompted the Tuscaloosa Water and Sewer Department to make some renovations.
City Water and Sewer Director Maurice Sledge said the spill was discovered after a man noticed a manhole discharging sewage from an area lift station near Cumberland Road.
The amount of sewage spilled is unknown, but city officials have speculated it was close to 500 gallons.
"I've got photos that prove otherwise," Wathen said.
Sledge said the spill occurred because a pump at the area lift station was not keeping up with flow maintenance.
After a crew vacuumed the sewage out of the manhole, a contractor was called in to replace the pump station with a portable device.
Although the pump has been repaired, the portable device is on standby in case another spill occurs, Sledge said.
Sledge made a trip down to the lift station and said he saw no sewage flowing into Hurricane Creek.
"It was dry when I got there," he said.
Wathen does not refute the circumstances surrounding the spill, but offered a reason as to why the creek was dry: Recent construction north of the creek has caused heavy amounts of silt to choke off the water supply.
Sledge said he notified the Alabama Department of Environmental Management and the Tuscaloosa Health Department of the spill. He also sent an e-mail to Wathen.
There are 60 area lift stations throughout the Tuscaloosa area and four of those stations will be "removed and decommissioned for more modern lift stations," Sledge said.
Sledge said the lift stations to be replaced are 25 to 30 years old.
Reach Cassandra Mickens at 722-0211.
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text:
Vegan Creamy Tomato Kale Soup
Be the first to review
READY IN: 40mins
Recipe by Mary M.
Creamy and bursting with flavor, this tomato soup has hidden vegetables to nourish the picky eater in the group.
See Discussion:
Ingredients Nutrition
- 1 (13 ounce) can tomato paste
- 1 (14 ounce) canorganic diced tomatoes
- 1 (6 ounce) canorganic tomato paste
- 2 cups kale, finely chopped
- 1 (13 1/2 ounce) can coconut milk
- 1 carrot (finely diced or grated)
- garlic powder, to taste. about 1/2-1 tsp
- to taste black pepper
- to taste sea salt or ono hawaiian salt
- 1⁄4 teaspoon dried red pepper flakes
Directions See How It's Made
- Place all cans tomato products into tall sided pot and bring to a simmer.
- Add all chopped veggies, spices and peppers. Bring back to a boil, then reduce to a simmer. Stir every few minutes as needed.
- Cook about. 15-30 minutes until veggies are of desired tenderness. The mixture should be thick like spaghetti sauce. More water can be added as needed, but keep on the thick side.
- Add can of coconut milk, including the water. And bring to a simmer then turn off heat
- Add salt to taste.
- Serve it up!


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Item specifics
Model Number: Airplane Audio Adapter
Type: Adapter Application: Audio & Video
is_customized: Yes Gender: other
The airne adapter converts a regular headphone stereo jack 1/8' (3.5mm) to a dual prong 1/8' (3.5mm) airne jack
Quantity: 20
Allow using your own 3.5mm audio jack headphone to enjoy the onboard radio, movies...etc
Fits all standard airne connectors and headphone stereo jacks
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If item DOA(Dead of Arrival),Buyers Can return it in 14 Days,We will Replace it with New item after we receive DOA item.Please Contact us Before you sent the item back Warranty:
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Product Description
Packaging Details Unit
Type: piece
Weight: 0.150kg (0.33lb.)
Package Size: 30cm x 28cm x 27cm (11.81in x 11.02in x 10.63in)
If you would like to pay by PayPal, you can pay to our PayPal account: firstname.lastname@example.com and email us. Please email us the name of the product, the quantity and other product attributes. The email address is firstname.lastname@example.com. No matter how many goods you buy in one order, the freight you need to pay is only 15 dollars
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Displaying 1 to 5 (of 5 reviews)
by Roger ZindukaManchester, GB
Date Added: 01/20/2017
Rating: [5 of 5 Stars]
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Date Added: 01/20/2017
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Rating: [5 of 5 Stars]
Displaying 1 to 5 (of 5 reviews)


text: The No Brainer Bracelet Put it on and stress just melts away... from the loss of circulation! Creative Talent Workshop December 05, 2010 82 Views More Info Full Credits Creative Talent Workshop Uploader Stats & Data 10Funny3Die 82 Views December 05, 2010 Published Category Sketch Parody Dumb People Tags parody commercial jewelry infomercial Franklin Mint brain brainless stupid people Up Next: The Russian Gymnast AUTOPLAY Up Next In: 10 The Russian Gymnast History of Rap: Eminem & Dr Dre Parody If You Want A Bail Out, A Hand Out! (Cat Stevens Parody) Yes, Madiera Follow Browse Related Comments 40+ more View All ▶ Creative Talent Workshop Vaguely Related Videos Vaguely Related Pics & Words Advertisement Tags: parody commercial jewelry infomercial Franklin Mint Advertisement


text:
Blueberry Orange Cobbler
Be the first to review
READY IN: 1hr
Recipe by Mims Squims
Serve warm with ice cream or whipped cream! Note: If you don't have & can't get maple sugar, use light brown sugar instead.
See Discussion:
Ingredients Nutrition
- 1 tablespoon butter, softened
- 4 cups fresh blueberries
- 1⁄2 cup orange juice
- 1⁄3 cup maple sugar
- 1 teaspoon vanilla extract
- 1 1⁄2 cups whole wheat flour
- 3 teaspoons baking powder
- 1⁄2 teaspoon sea salt
- 1 tablespoon maple sugar
- 6 tablespoons butter, frozen (in one piece)
- 3⁄4 cup orange juice
Directions See How It's Made
- Heat oven to 425°F; spray 10-inch deep dish pie plat with non-stick cooking spray.
- In bowl, toss together blueberries, maple sugar, orange juice, and vanilla. Let stand 15 minutes.
- Pour into pie dish and bake 12 minutes.
- In separate bowl, whisk together flour, baking powder, salt, and sugar. Grate frozen butter over flour mixture and stir. Add orange juice and loosely blend. Do not overmix.
- Spoon dollops batter over baked blueberries. Sprinkle with sugar.
- Bake 10-15 minutes until top is golden and fruit is bubbly.


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text: 4/23/10 4/22/10 POPSUGAR Celebrity Jessica Simpson Slide Picture of Jessica Simpson Leaving Hotel in New York Besties April 22, 2010 by Celebrity 0 Shares Chat with us on Facebook Messenger. Learn what's trending across POPSUGAR. Jessica Simpson left her NYC hotel with Kan Paves on Wednesday. Image Source: Bauer-Griffin Online HotJessica Simpson


text:
Dr Yiliang Ding
Project Leader
Cell and Developmental Biology
Post-transcriptional regulation of genes has gained much attention in recent years because of its direct and rapid regulation of protein levels. Our previous research has developed two novel and powerful platforms to study RNA structure in vivo and across diverse species at both the genome-wide scale (Ding et al., 2014 Nature) and in individual RNAs (Kwok et al., 2013 Nature Communications). These breakthroughs have removed technological barriers and opened up new avenues for RNA biology research. Our lab utilizes multidisciplinary knowledge and approaches in areas of Nucleic Acid Chemistry, RNA Biology and Bioinformatics.
Our research focuses on:
1. Determining the role of RNA structure in the post-transcriptional regulation of gene expression including translation, polyadenylation, RNA splicing, RNA stability and RNA processing.
2. Exploring how RNA structures alter in response to environmental changes.
3. Developing new experimental and analytical approaches for revealing RNA structure and RNA-protein interactions.
Our lab is seeking enthusiastic and highly motivated people with interests in studying these new avenues.
ContactTel: 01603 450266
Recent Publications
FoldAtlas: a repository for genome-wide RNA structure probing data
Bioinformatics Sep 22 2016 p1-3
Publisher's version: 10.1093/bioinformatics/btw611
The NIN Transcription Factor Coordinates Diverse Nodulation Programs in Different Tissues of the Medicago truncatula Root.
Plant Cell 27 p3410-24
Publisher's version: 10.1105/tpc.15.00461
Genome-wide profiling of in vivo RNA structure at single-nucleotide resolution using structure-seq.
Nature protocols 10 p1050-66
Publisher's version: 10.1038/nprot.2015.064
A stable RNA G-quadruplex within the 5'-UTR of Arabidopsis thaliana ATR mRNA inhibits translation.
Biochemical Journal 467 p91-102
Publisher's version: 10.1042/BJ20141063
In vivo genome-wide profiling of RNA secondary structure reveals novel regulatory features.
Nature 505 p696-700
Publisher's version: 10.1038/nature12756
Yiliang Ding
- Dr Qi Liu Postdoctoral Scientist
- Dr Matthew Norris Postdoctoral Scientist
- Dr Xiaofei Yang Postdoctoral Scientist
For media enquiries, please contact the JIC communications team 01603 450962, firstname.lastname@example.com


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Fiber Logic offers carrier-class digital microwave equipment for wireless connections of carriers' backbone networks. The digital microwave can be quickly deployed to supplement the fiber optical networks, forming a very effective IP/ATM/SDH transport network. The system capacity of our microwave equipment include 4~16 T1/E1, E3/T3, and OC-3/STM-1 with frequency coverage of 2~11GHz.


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Web Designers Derby Online Design Copyright © 2017 Online Design UK. All rights reserved.


text: House of Oracle is under construction. The author, Zicoihno is working hard to finish it! This article, House of Oracle, is property of Zicoihno. House of Oracle Kanji 託宣の家 Romaji Ie no Takusen Status Operating Structure Founder(s) Eden King Headquarters Brickham Member(s) ???? Affiliation Oracle Network Purpose Manage high risk, crisis scenarios and distribute classified intel to the highest, legal bidder. "We clean up messes for people with way too much time and money on their hands. And the occasional intel job. " — Eden King, founder and Chief. The House of Oracle (託宣の家, Takusen no Ie), normally referred to as simply Oracle, is a private, crisis management and intel firm based in the city of Brickham, a coastal, metropolitan financial hub of a yet named nation on the Yorbian Continent. It was founded some eight years ago by Double Star Hunter Eden King to better facilitate his intel business endeavors and, more specifically, consolidate his work in crisis management and build up a base of subordinates to better handle the needs of clients. This article is a stub. You can help Hunter x Hunter Fanon Wiki by expanding it. Retrieved from "http://hunter-x-hunter-fanon.wikia.com/wiki/House_of_Oracle?oldid=5531" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Under Construction Article stubs Organization Zicoihno Add category Cancel Save


text: One of the main things which makes Ableton unique amongst DAW's is it's ability to perform nearly every process while playing, or if you like 'Live'. This capability is what has made Ableton so popular with producers and DJ's who want to perform live and incorporate their own productions with other music. Session view is where all the creative stuff happens and it's functionality enables quick, creative and intuitive capture and manipulation of your musical ideas. In mastering session view you will unlock the real power of Ableton as a creative tool and at the same time beigin to experience what it's like to have software working for you rather than inhibiting your artistic flow.


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Louis Rousselet Glass Necklace, Pink Green, 1940s VintageLouis Rousselet
Product Information
Pretty pink milky glass beads with alternating small peking glass beads and darker green/brass discs. From the French designer Louis Rousselet who was one of the major sources for glass beads.
Measurement: 17"
All of our jewellery is unique, vintage second hand pieces individually sourced by Liz unless otherwise stated.
Free Delivery on orders over £50


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Badrinath Ki Dulhania (2017)
- Release Date: March 10, 2017
- 292
Running Time: min
Director: Shashank Khaitan
Star Cast: Varun Dhawan, Alia Bhatt, Gauhar Khan
Description:Badrinath Ki Dulhania bollywood hindi movie is a indian Comedy, Romantic film. Star cast actor Varun Dhawan and actress Alia Bhatt, Gauahar Khan in lead roles. This movie directed by Shashank Khaitan. Produced by Apoorva Mehta, Hiroo Yash Johar, Karan Johar. Written by Shashank Khaitan. The film distributed worldwide by Fox Star Studios.More DetailsWatch Trailer


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For more information, choose a topic above.
In this short video clip, Bob (a former patient) talks about his time in Intensive Care. You can read interviews,listen to voice recordings and watch clips of other patients' experiences of Intensive Care by using the link to a free website called Healthtalkonline.


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This article is a stub. You can help The Legend of Dragoon Wiki by expanding it.
|Home Town
Drippy is the town drunk of the city of Fueno in the game The Legend of Dragoon. It is unknown whether Drippy is actually his real name. He appears only after Dart and Rose have reunited with their friends, after being violently thrown off a ship.
He appears only in Disc 2 but is a very memorable character, due to his endearing personality and sense of danger. The townspeople respect his opinion and value him as a member of their society. He aids Dart and his friends in their quest by divulging some important information to them on their way out of Feuno. He is the only person responsible for telling them that a dragon (and Lenus, as well) have appeared on Prison Island. Without this, Dart and Shana would not have been able to continue forth.
Appearance Edit
Drippy wears a yellow cloak, similar to many of the other townspeople, with a distinctive yellow hood. He can be easily recognized by his dapper appearance, as well as the bottle of liquor he carries in his right hand. He is rarely seen without his bottle, and driving some people to perceive him as more of a drunk than he really is. Truth be told, he is really just protective of his stash. He is a solid, reliable companion, brave and dedicated to the defense of his town.
Personality Edit
Drippy has a swagger when he walks, mostly due to his alcoholism, but also partially because of his injury. When Drippy was young, he fell down a set of stairs and unfortunately broke his hip. While it has been fixed, he still pays for his klutziness by constantly having a limp when he walks. He is a loud individual, and townspeople can hear him coming for miles. He is easily identified by his signature sound; "moan - hiccup".


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If you are not quite ready for scuba diving but don't want to miss out on the fun, this experience is the answer. All you need is a space-age helmet that lets you breathe as you stroll along the seabed in diamond-clear waters and hand-feed the fish.
Shallow water helmet diving is the safest underwater adventure anyone can undertake. There is a constant flow of fresh air pumped into the helmet and the excess bubbles flow freely out of the bottom of the helmet. There is no restricting regulator or snorkel in your mouth to cause any feeling of discomfort or anxiety. You can even keep your glasses or contact lenses on!
As you stroll in 10ft of clear turquoise water you can observe the sea life as it swims around you, see wonderful rock formations and have the opportunity to feed the fish. You don't even need to be able to swim. A short introduction is all that's needed, and with all the gear left on board, you don't have to worry about heavy tanks.
Departs: Daily, except Sundays.
Duration: 2 hours
Hotel Pick up: Starts at 9.00am from hotels in Protaras and Ayia Napa
Return transfer from hotels in Protaras and Ayia Napa
Departs: Daily, except Sundays.
Duration: 2 hours
Hotel Pick up: Starts at 9.00am from hotels in Protaras and Ayia Napa. Your exact pick up time will be advised upon confirmation.
Dress Code/What to Bring: Don't forget your swimsuits, towels and sun protection.
Child Policy: Child needs to be at least 7 years old to participate, and must be 1.2 meters tall.
Please not that this experience is not suitable for pregnant ladies.
9.00am - 9.25am: Pick up from Protaras and Ayia Napa hotels
9.25am - 9.50am: Drive to Ayia Triada beach and take a boat ride to the platform
9.50am - 10.00am: Safety Briefing & wet suits & boots given.
10:00 - 10:30 :
Proceed with Under Sea walk (group 1), group 2 has free time to snorkel (snorkels free of charge)
10:30 - 11:00 :
Proceed with Under Sea walk (group 2), group 1 has free time to snorkel (snorkels free of charge)
11:00 - 11:30 :
Back from platform to shore and customers get shown their movie and photos
11:30 - 12:00 :
Transfer back to hotels.
Departs: Daily, except Sundays.
Cost: €47 per adult, €37 per child (7-11 years)


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Losing stakes: One in five gambling addicts are suicidal, new report finds
Counselling centre research finds 'basic issues of sleep and work' behind problems
About 20 per cent of gambling addicts who sought help at a counselling centre had considered suicide, while 22 also thought of killing their families, a report says.
Researchers from the University of Hong Kong found that of the 3,685 people who sought help at the Caritas Addicted Gamblers Counselling Centre between 2003 to 2012, about one in five had thought of attempting suicide and 22 individuals had thought of taking their family members' lives as well.
"When we look at the reasons behind suicidal thoughts among gamblers, it is easy to overlook the basic issues of sleep and work," said Paul Wong Wai-ching, social work and social administration assistant professor at the university.
Among the suicidal group, they found that 57 per cent said of gambling addicts suffered insomnia and 43 per cent said they were unmotivated at work. In the non-suicidal group 33 per cent suffered insomnia, while 26 per cent felt unmotivated.
The researchers also found that addicted gamblers usually face multiple problems in life, including emotional and family problems that affect their sleep. A lack of sleep may also lead to impulse behaviour, they said.
The researchers warned gamblers and their families to be aware of these signs which may be point to future bahaviour.
The report is to be published in international journal Crisis: The Journal of Crisis Intervention and Suicide Prevention, next month.
Last week, suicide prevention workers said that ingrained resistance to discussing mental health problems and suicide in Hong Kong is proving to be an obstacle to bringing down the rising rate of young people killing themselves.
The city's youth suicide rate rose by 19 per cent between 2010 and 2012. In 2010, it was 7 in every 100,000 youths, but the number rose to 8.3 in 2012.
Meanwhile, the overall suicide rate dropped 8 per cent – from 13.8 in 2010 to 12.7 in 2012, according to the University of Hong Kong's Centre for Suicide Research and Prevention.
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Morning everyone, lots of work still going on in the county following last week's tidal surge and coastal flooding.
Many of the roads that were closed have already reopened but we have some updates for you. The A143 Haddiscoe reopened this morning after flood water subsided and the road was swept and the Quay at Blakeney is expected to open later today after inspection.
Work is continuing on the B1159 Coast Road, Walcott, and the A149 Coast Road in Salthouse where excavators and lorries have been working since daylight to remove sand, silt and debris. The state of these roads will be reassessed later this morning.


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Herbed Chevre, Craisin and Walnut Pinwheels
Recipe by BecR2400
These festive pinwheel appetizers are perfect for the holidays, or anytime! Very popular with my family and guests---everyone really loves the great flavor combo of tangy chèvre cheese (or feta), sweet-tart cranberries, green onions, walnuts (or pistachios), and herbs. Easily made in advance, and I have frozen the logs (before slicing) with great results. Note: If you can't find chevre cheese, crumbled feta is equally good. A slight modification of recipe 146700-- thanks Wildflour!
Top Review by Mudflower
This filling is wonderful. The goat cheese flavor is not too strong, but you can definitely taste it. I did not make it with the tortillas, but instead made some simple table water crackers and served it with those. A classier appetizer that is very easy (Except for the cutting, my 4 year old could have made it, really).
See Discussion:5 Reviews
Ingredients Nutrition
- 2 (8 ounce) packages cream cheese, very well softened
- 1 (5 1/2 ounce) packagechevre herbed goat cheese (equally good with crumbled feta or plain chevre, with a pinch of Herbes de Provence or to taste)
- 1⁄2 cup finely chopped green onion
- 6 ounces dried sweetened cranberries (Craisins)
- 3⁄4 cup chopped walnuts or 3⁄4 cup unsalted pistachios
- 4 large flour tortillas
Directions See How It's Made
- In medium bowl, combine softened cream cheese, chèvre cheese (or feta), a pinch of Herbes de Provence (if using), green onions, dried cranberries and chopped walnuts (or unsalted pistachios).
- Evenly spread 1/4 of the mixture onto each tortilla, all the way to the edges.
- Roll up tightly, being careful not to tear.
- Wrap each in plastic wrap; refrigerate for several hours or overnight.
- Slice to serve. Makes about 32 appetizers.


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Cleanrooms Australia is committed to the environment and minimizing the environmental impact of our installations.
We are conscious of the environment, and always mindful of our personal responsibility to take steps within our control to ensure minimal damage to the planet.
We believe that we can gain the best results in design by using a holistic approach in concert with the client's needs, and government guidelines.
We have been recognized within the industry for creating projects that are sustainable environmentally as well as economically. Below is a brief summary of a project that we have been recognized for.
The installation of a cleanroom was undertaken in a warehouse space attached to the proprietor's office space. Due to the high electrical demand of this type of installation and the fact that the area has electrical distribution issues related to high density housing and varying power demands, during both the design and the construction phase attention was made to minimizing the electrical burden.
The potential obstacles that were addressed:
- The introduction of outside air for pressurisation and make up air has the potential of being the largest heat load due to moisture content during the cooler months.
- The removal and control of the humidity is a substantial expense in the operation of the facility
- If you choose the less expensive capital cost design of cooling the supply air and reheating, the required power consumption is high.
The alternative is to use a freestanding direct expansion refrigeration and desiccant wheel dehumidification unit, although a higher capital cost, the return on investment was calculated at about 5 years depending on the design parameters.
The benefit of the dehumidification unit is that you only need to address the small volume of incoming air, rather than treating the large volume of recirculated air inherent to a cleanroom application, and the power saving is significant, reducing the electrical burden to the environment from this cleanroom as well as saving thousands of kilowatt of costly power for the owners.


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Debt-ridden pub chain Punch Taverns has seen its share prices fall even further, as details of its newest refinancing package were announced.
The company's £2.3 …
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29 May
Debt-ridden pub chain Punch Taverns has seen its share prices fall even further, as details of its newest refinancing package were announced.
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27 May
A study undertaken by consultancy firm, Brand Finance, has discovered which football club can claim to have the strongest identity amongst consumers.
Although six of …
22 May
Australian women who undertake maternity leave may end up paying an additional 30% in student loan interest, under proposed education reform.
Although the change has …
20 May
A new report has suggested that people approaching retirement need to plan for an annual income of at least £15,000 a year, in order to …
15 May
The financial ombudsman has warned that many homeowners are in denial about their mortgage debt, causing many to face the threat of repossession.
With a …
13 May
New research has shown that a third of people have suffered mental health concerns as a result of debt problems.
Surveys carried out in conjunction …
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Two leading football clubs, Manchester City and Paris Saint-Germain, could face millions of pounds in fines as a result of breaking UEFA's financial fair play …
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Want to check your balance? There's an app for that. Want to calculate your mortgage repayments? There's an app for that. Want to pay a …
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Disgraced Birmingham City owner, Carson Yeung, convicted in March this year on a charge of money laundering, is facing further charges from a Hong Kong …
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Sources have suggested the Conservative party will be able to declare itself debt-free in the build-up to the general election next year.
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When I started transitioning I always dreaded 'wash days'... They were always long and boring or maybe I was too impatient buh by the time I got rid of the relaxed ends and knew my hair a little more, I figured ways to cut corners and products that worked perfectly well. I'm an Eden … Continue reading How I Cut My WashDay Routine In Half;The Night Before Valentines
Protective Styling:Avoiding Some Horrors
Hey guys.... As much as I love protective styles(because they help you retain length,save your hair from harsh weather,save you the trouble of trying to figure out something with your hair & a host of other advantages..),I've been a victim one too many times of a particular protective style gone wrong (weave-ons), and yes I … Continue reading Protective Styling:Avoiding Some Horrors
Ready, Jet, Inspiring Lights!
Hey Guys... I know we have been MIA on here but it's for good reasons...too much has been going on- good stuff tho!! So recently I went to New York *insert Alicia Key's 'Empire State of Mind'* for a friend's event, she hosted the Miss Nigeria USA Pageant and it was super fun!!(see more pictures … Continue reading Ready, Jet, Inspiring Lights!


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|Home | About | Journals | Submit | Contact Us | Français
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
It has been demonstrated that a reliable and fail-safe sequencing strategy is mandatory for high-quality analysis of mitochondrial (mt) DNA, as the sequencing and base-calling process is prone to error. Here, we present a high quality, reliable and easy handling manual procedure for the sequencing of full mt genomes that is also appropriate for laboratories where fully automated processes are not available.
We amplified whole mitochondrial genomes as two overlapping PCR-fragments comprising each about 8500 bases in length. We developed a set of 96 primers that can be applied to a (manual) 96 well-based technology, which resulted in at least double strand sequence coverage of the entire coding region (codR).
This elaborated sequencing strategy is straightforward and allows for an unambiguous sequence analysis and interpretation including sometimes challenging phenomena such as point and length heteroplasmy that are relevant for the investigation of forensic and clinical samples.
Investigations of the human mt genome are in the focus of biological and medical scientific disciplines. Compared to nuclear DNA (nDNA), mitochondrial DNA (mtDNA) is more vulnerable to oxidative damage and undergoes a higher rate of mutation [1]. Because of these features the analysis of the mt genome has become a proven tool in population genetics. A multi-copy genome without recombination which accumulates mutations allows for the establishment of phylogenetic trees [2]. It was the information from the highly variable mitochondrial control region (CR) that lifted the secret of human evolution starting in Africa about 150000 years ago and gave an insight in human migration all over the world within the past 60000 years [3,4]. Sequences of full mt genomes are necessary to decipher yet not defined haplotypes and assign them to their phylogeographic environment.
Mitochondrial DNA mutations in the coding region (codR) have been associated with several pathologies [5] including cancer [6-9]. During oxidative phosphorylation (OXPHOS) mitochondria produce reactive oxidative species (ROS) that potentially induce DNA mutations. Such an initial mutation is heteroplasmic with the mutated variant constituting a minority [10]. In the course of several replications the heteroplasmic mutation may become dominant leading to cancer [8]. This theory is based on the results of several investigations on cancer tissues [11-18]. Unfortunately, numerous articles addressing that issue are erroneous as reviewed in [19,20]. On the one hand it is the lack of phylogenetic knowledge and the ambiguous mtDNA alignment that led to false conclusions of mtDNA mutations to be tumor-specific rather than evolutionary caused. On the other hand, laboratory-, sequencing-, and analysis errors led to wrong base-calls [21]. Hence, flawed data hamper a precise interpretation of the conjunction between mtDNA mutations and the complex process of tumor development.
For forensic as well as for phylogenetic purposes we have already successfully established evaluated sequencing strategies that proved to be useful in a number of investigations where precise base-calling was necessary for the CR [22-26], however such stringency is lacking for the whole mt genome. The published protocols vary concerning the number and sizes of PCR products, the chemistry employed, and the number of sequencing primers [27-31]. One review reports the use of 58 sets of unique sequencing primers to completely cover the mt genome, while another protocol provides 77 sequencing primers for the codR and 7 additional primers for the CR [27]. There, sequencing is performed on 12 amplicons that cover the whole mt genome in an overlapping manner [29]. In a recent protocol [28] the amplification of the entire mt genome was conducted with only two overlapping amplicons, followed by 48 upstream and downstream sequencing reactions. Whereas amplicon sizes must be kept short for forensic samples for reasons of limited DNA quality and quantity, a reduction of the necessary amplicons is desirable for other applications, where usually fresh DNA is obtained. This simplifies the laboratory work and minimizes potential amplicon mix-up [19]. Independent of the amplification strategy high sequence quality is required to achieve reliable base-calling.
We addressed this issue by presenting a set of 96 carefully selected sequencing primers that are embedded in a reliable and fail-safe sequencing strategy. The following criteria were applied to guide the development. (1) Each nucleotide reported in the consensus sequence should derive from at least two independent sequencing reactions using different primers (double strand coverage) to avoid the reporting of phantom mutations and other ambiguous base-callings. (2) We envision a minimum number of PCR products to reduce the chance for amplicon mix-up during the (manual) set-up of sequencing reactions and (3) we selected primers that produce sequences with an optimal signal-to-noise ratio to enable unequivocal assignment of point and length heteroplasmy.
The complete mtDNA was amplified using 2 overlapping fragments each about 8.5 kb in length with primers published in [28]. Fragment A ranges within nucleotide positions 2499 and 10837, fragment B between 10672 and 2669 comprising the CR (Table (Table1,1, Figure Figure1,1, step3).
The sensitivity of the amplification reaction was elicited with 1000, 2500, 5000, and 10000 molecules of quantified mtDNA [32]. PCR yield and specificity were visualized by polyacrylamide gel electrophoresis (Figure (Figure2).2). Even though the density of the banding pattern varied significantly (depending on the amplified DNA amount), the sequence analysis of all fragments resulted in comparably clear data (Figure (Figure3,3, right). Also the amount of 1000 mtDNA genomic equivalents (GEs) that roughly corresponds to the mtDNA content of a single lymphocyte (fresh sample) proved sufficient for reliable results (given that the mtDNA is intact). PCR products showed light smear on the polyacrylamide gel (Figure (Figure2),2), especially with increasing template amount. However, we did not observe negative effects on the quality of the sequencing results (Figure (Figure3).3). No evidence of contamination was observed, neither in the non-template controls nor during evaluation of the individual mtDNA haplotypes. Both extraction blanks and PCR negative controls were free of signal. The mtDNA of two tissues (breast and blood) was extracted, amplified and sequenced from each person at different times and corresponding haplotypes gave a match after comparison (the haplotypes did not match laboratory staff).
Amplicons were purified according to two different protocols. We compared an enzymatic digestion method with a filtration method (Figure (Figure2).2). We did not observe significant differences with respect to the DNA amount after purification except for one sample. There, the banding signal of the ExoSAP-IT purified product was less dense as compared to the filtrated one. However, despite of the different banding intensities (Figure (Figure2)2) we did not observe relevant changes in sequencing quality between the electropherograms (Figure (Figure3).3). The filtration method has the appealing advantage that it is less expensive for PCR product purification of few samples present in large volumes (up to 0.5 ml). One Microcon column is needed for each sample irrespective of the DNA volume to be added, whereas the required amount of ExoSAP-IT is required to be proportional to the volume of DNA (2 μL per 5 μL PCR-product).
We established a set of 96 sequencing primers that lead to full double-sequence coverage of the complete mtDNA codR (Figure (Figure4).4). Primer sequences were partly taken from [29,33] and http://insertion.stanford.edu/primers as well as designed inhouse [34]. In combination with sequences obtained from the CR [35] this resulted in high-quality sequence information for the whole mtDNA genome. Such a strategy makes sense, as full mt genome sequencing is usually carried out on selected samples that have earlier been analyzed within the CR. Sequencing was performed on the basis of the Big Dye Terminator Ready Reactions Kit protocol (Applied Biosystems, Foster City, CA). The quality of the primers was generally stable despite of multiple freezing/thawing cycles of the stock primer plate. For 6 primers (R4, R7, R18, R20, R21, F14) out of 96 the signal-to-noise ratio decreased after the fifth freezing/thawing cycle. An example of the general quality of the sequences is shown in Figure Figure5.5. The sequence is displayed by two primers in both directions confirming point heteroplasmy of the variants A and G at position 2673 within the NADH dehydrogenase (ND2) gene.
Sequence electropherograms did not differ substantially in terms of the signal-to-noise ratio as well as with respect to the relative peak heights when comparing the tested mtDNA template amounts. Although a relative increase of the fluorescence signal in the raw data was detected (Figure (Figure3,3, left), the quality of the sequence electropherograms were comparable (Figure (Figure3,3, right). In this study we tested template mtDNA amounts between 1000 and 10000 GEs that turned out to cover an appropriate template amount range for long PCR fragments.
Upon analysis of the raw data the sequences were aligned and the base-calls reviewed twice by two independent scientists, such as has been found invaluable for CR analysis [35]. Consensus sequences were aligned and compared to the revised Cambridge Reference Sequence (rCRS) [36,37] following nomenclature guidelines for mtDNA typing [38-40]. In an independent analysis the two consensus sequences underwent comparison by means of a dedicated in-house software [41,42]. This concept enabled full electronic data handling minimizing the risk to introduce clerical errors.
We present the complete mtDNA sequences of 10 clinical samples from 5 patients with respect to the phylogeny [43] (Figure (Figure6)6) [sequences were deposited in GenBank http://www.ncbi.nlm.nih.gov/Genbank/ with accession numbers FJ384431–FJ384440]. These patients were classified as typical West-Eurasian lineages as members of haplogroups W1d, T2b, V4, H5, and H15b [33,44,45]. As shown in Figure Figure66 (highlighted in light grey), the mtDNA sequences generated from the primary cancerous tissue and the peripheral blood cells were identical in all 5 patients.
We present a reliable laboratory strategy for the amplification and sequence analysis of the full mt genome that meets a high quality demand. The method is conceived for samples that include an adequate amount and quality of DNA, such as found in fresh clinical and forensic samples and can be conducted by any laboratory in the molecular field without the requirement for automated liquid handling devices. The complexity of the laboratory concept is low, so that handling errors and risk of sample mix-up and contamination are minimized.
It has been shown in this study that the sequence performance of the majority of primers was not affected by different storage conditions. Only 6 primers (R4, R7, R18, R20, R21, F14) suffered from a treatment of more than 5 freezing/thawing cycles. Temperature changes caused a diminished function visualized by a disadvantageous signal-to-noise ratio depicted in the electropherogram. The ability to correctly identify point heteroplasmy strongly depends on the quality of the overall signal height and the signal-to-noise ratio. Consequently, an apparent improvement of the storing conditions would be the generation of stock plates including less primer volume sufficient for about 5 sequencing reactions. Alternatively, sequencing primers could be kept in a low tris buffer at 4°C.
Recent research has been addressing the role of mitochondria and the mtDNA in aging and cancer as mitochondria participate in fundamental processes of the cellular metabolism [46]. The mitochondrial theory of aging implies that tissue function sustains until the number of cells declines below a threshold. The time range required to reach this threshold is related to the rate at which mitochondrial and mtDNA damage accumulates. If the mitochondrial ROS production rate increases, the rate of cell loss will also increase, resulting in early tissue failure and age-related disease.
Not only aging processes but also carcinogenesis have been linked to mutation based mitochondrial dysfunction since a general feature of tumor biology is the impaired energy metabolism [47]. For those investigations addressing aging and tumor biology, mitochondrial genes encoded in the nucleus and in the mitochondrial DNA are being analyzed. Genes encoded in the mitochondrial DNA can be systematically targeted with this evaluated sequencing strategy.
Furthermore, it can be helpful for population genetic and forensic applications where further information to the CR is required. In the forensic context this is particularly relevant for the few CR haplotypes that are shared more commonly within a population. In the West Eurasian population this is the case for the CR haplotypes 16519C 263G 315.1C and 263G 315.1C, which occur at frequencies of 0.013 and 0.002, respectively [48]. When such haplotypes are observed in a forensic case between suspect and crime scene samples one needs to consider the possibility that the sequences are identical by state and not by descent. Especially, as it is known that these haplotypes occur in more haplogroup backgrounds, such as H1, H2a, H3, H7, H10, H13a1, HV, R0 and the great bulk of yet unidentified sub-H lineages [26,49]. To increase the information content of such haplotypes we have earlier introduced a screening method based on single-base-extension reactions that target informative SNPs (single nucleotide polymorphism) in the codR [50]. Such screening methods are a valuable supplement to standard mtDNA sequencing as they generally increase the discrimination power and indicate the hg-affiliation of an mtDNA haplotype [49]. The logical continuation of this strategy would be the analysis of entire mt genomes in forensic casework for achieving maximum discrimination power. The current sequencing technology allows that only for relatively high sample qualities, but it is a first step towards mt genome sequencing in forensics.
In the population genetic field an accurate deciphering of the human mitochondrial phylogeny can only be conducted on the basis of full mt genomes. As a general approach full genome mtDNA sequencing is carried out on a defined selection of CR sequences as evidenced by recent examples [51,52].
The focus of this strategy lies on applications where secure base-calling and high quality sequence data are mandatory for interpretation. It is well known that mitochondrial mutations associated to disease such as mitochondrial encephalomyopathies occur in heteroplasmic status and that the severity and the progression of the syndrome depend on a threshold above which the mutant triggers the pathological pathway [53,54]. The contribution of mtDNA mutations to carcinogenesis underlies the same assumption. It appears evident that particular mitochondrial defects with functional consequences exhibit an advantage in tumor development only if a certain threshold of mutated mtDNA populations is achieved. All the more it is important to pinpoint the relative quantity of these heteroplasmic mutations to estimate functional consequences of the genes involved. Moreover, good sequencing quality is the prerequisite to distinguish early stage point heteroplasmy from signal background which might be an issue in early stage cancer detection [55].
The occurrence and the frequency of mtDNA control region (CR) point heteroplasmy is an important issue in forensic case work [56] as the detection of point heteroplasmy, especially at low level, increases the power of discrimination between sequences [57].
The codR sequencing method described herein is an optimized protocol that can also be applied in laboratories that do not have automated processes available. The overall aim, namely the achievement of a secure base-calling method was accomplished by the assortment of primers that allow for full double-stranded sequence coverage of the whole mtDNA genome (in combination with previously described CR sequencing strategies). Particular care was taken on the selection of the primers based on low background signal that is crucial for the unambiguous assessment of length and point heteroplasmy. Moreover, we kept the complexity of the laboratory process as low as possible. This was achieved by a 96- well based pipetting format for cycle sequencing set-up using only two PCR amplicons per sample which reduces the chances of contamination, handling error and sample mix-up.
A total of 10 biopsy samples were collected from patients with diagnosed invasive mamma carcinoma at the Clinical Department of Radiology, Innsbruck Medical University. They were immediately transferred into 1.5 mL vials and extracted or frozen at -20°C and extracted within the next 7 days. Corresponding blood samples were collected independently. Peripheral blood samples were either extracted upon receipt or frozen at -20°C and then processed within 7 days. There was full consent of the patients to participate in this study. For the sensitivity study DNA was extracted from peripheral blood of a healthy volunteer of European ancestry with informed consent.
The complete workflow is sketched in Figure Figure1.1. DNA was extracted from biopsies and blood via the EZ1 Biorobot workstation (Qiagen, Hilden, Germany). Extraction was based on the protocol of the investigator kit (Qiagen) for extraction of blood and tissue samples, with modifications as follows: tissue samples (about 0.5 cm × 1 mm) were digested with 15 μL proteinase K in 290 μL lysis buffer (included in the kit) up to 4 hours and treated with the "trace" protocol.
A total of 96 primers were used for singleplex reactions. Primer sequences were taken from [29,33] and new primers were designed in this study with PRIMER 3 software [34]. Possible heteroduplex formations, primer dimers and hairpin structures were analyzed with OligoAnalyzer free software: http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/ (Integrated DNA Technologies, Coralville, IA). Melting temperature of primers was approximately 50°C and the GC-content was between 40 – 60%. The settings for the calculations were: Oligo conc. n: 0.25 μM; Na+ conc.: 50 mM.
For amplification we used the Advantage GC Genomic LA Polymerase(Clontech, Bella Avenue Mountain View, California) including a small amount of proofreading enzyme, a hot start antibody and a 3' to 5' proofreading exonuclease activity. It enables synthesis of PCR products of 8.5 kb using human genomic DNA templates.
Amplification reaction was carried out on a thermal cycler (Multicycler PTC240 Tetrad2, Hercules, CA) in a total volume of 50 μL each fragment (A and B) comprising 2.5 mg/mL BSA (St. Louis, Missouri), 2.5 mM each dNTP (Applied Biosystems), 10 mM each primer, 5U LA Genomic DNA polymerase (Clonetech) 1000 – 10000 mtDNA GEs. Thermal cycling conditions comprised an initial 3 min denaturation step at 93°C, followed by 93°C for 15 s, 60°C for 30 s and 68°C for 5 min 14 times proceeding 27 times with 93°C for 15 s, 55°C for 30 s and 68°C for 9 min increasing for 10 s each cycle. Amplicons were purified from residual primers and dNTPs enzymatically with ExoSAP-IT (ExoSAP-IT, USB, Cleveland, Ohio) and comparatively with a filtration method (Microcon YM-30 Centrifugal Filter Units: Millipore, Billerica, Massachusetts).
From a 1.5 μM stock plate containing all 96 sequencing primers (Figure (Figure1,1, step 4), 2 μL were decanted into a new plate right before each sequencing reaction. Big Dye Terminator mastermix (containing 1 μL BigDye Terminator v1.1 Cycle Sequencing mix (Applied Biosystems) and 3 μL BigDye Terminator v1.1 Sequencing Buffer (Applied Biosystems)), as well as 2 μL mtDNA Fragments A or B were aliquoted into the appropriate wells as shown in Figure Figure11 (step 4). 2 μL distilled water was added to reach a final volume of 10 μL. Pipetting those small volumes was performed using an 8-channel epMotion workstation (Eppendorf AG, Hamburg, Germany). Cycle sequencing was performed (after a first denaturation step of 95°C for 1 min) for 25 cycles of 10 s at 95°C, 5 s at 50°C, and 4 min at 60°C.
Sequencing reaction products were purified from residual dye terminators using Sephadex G-50 Fine (Amersham, Buckinghamshire, United Kingdom) and Multiscreen filter plates (Millipore) according to the manufacturer's protocol. The cycle sequencing products were diluted by adding 10 μL of distilled water and the dilutions were centrifuged through the filter plate into an optical 96-well plate for electrophoretic separation. The entire procedure of diluting cycle sequencing products and transferring the dilutions onto the Sephadex columns in the filter plate was again performed by the epMotion workstation. When spinning cycle sequencing products through the filter plate, unequal amounts of product may be recovered throughout the plate. In order to avoid this, the blocks' orientations in the centrifuge carriage were reversed after 2.5 min and the blocks were spun a second time for 2.5 min to obtain consistent amounts of purified products. The purified products were finally diluted by adding each 20 μL of distilled water to achieve volumes of 45 μL.
Electrophoretic separation was carried out on an ABI 3100 capillary sequencer (Applied Biosystems) using POP6 and a 36 cm capillary array. The run module conditions were as follows: injection time: 22 s, injection voltage: 1 kV, run voltage: 15 kV, run current: 10 μAmps, run temperature: 55°C.
LF developed the whole mt genome sequencing strategy, coordinated the experiments and drafted the manuscript. BZ and MD performed experiments. WP participated in study design, supervision, and revision of the manuscript. All authors read and approved the final manuscript.
The authors would like to thank Birgit Krabichler (Sektion für Humangenetik, Medizinische Universität Innsbruck) and Martin Bodner (Institut für Gerichtliche Medizin, Medizinische Universität Innsbruck) for valuable technical assistance and discussion. We would further like to acknowledge three anonymous reviewers for helpful suggestions. This research received support from the "Österreichische Forschungsförderungsgesellschaft, KIRAS Sicherheitsforschung DNATOX (813786/12828-GLE/SHJ)" and from the FWF Austrian Science Fund (TR-L397).
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Invisible Inc.
Written by Steve Cole
Illustrated by Jim Field
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The Lovereading4Kids comment
In 3 : invisible goodies – dastardly baddies – killer chickens (poultry-geists!) Steve Cole is a national treasure: creator of series such as Astrosaurs and Magic Ink, and now writing the Young Bond books, he's delighted huge numbers of young readers with his ever inventive, clever, often very funny books and stories. Invisible Inc, in which a young boy is accidentally made invisible and then joins forces with a Victorian lady scientist, medieval knight and horse (all also invisible) to save the world, has all his trademarks: the action is fast and furious, the jokes are many and varied, and it all feels absolutely up to date, exactly the kind of book that today's young readers want. Jim Field's illustrations are the perfect complement to the text adding extra tension or humour as required. ~ Andrea Reece
Invisible Inc. by Steve Cole
Chaos, wizardry and a gang of kind-of ghosts, in this hilarious adventure from bestselling author, Steve Cole. Noah's mum's new invention can zap ANYTHING into a ghost of its former self. It's still there, but you can't see it. You can't touch it. When the sinister 'Seerblight Solutions' steal her invention, Noah is zapped - and finds he's not the first to have been turned 'invisible' through the ages. With all humankind in terrible danger, Earth's last line is defence is one you've never seen or heard of: Invisible Inc. A medieval knight. A Victorian inventor. A poetic pony. And an ordinary boy. Saving the world? THEY'LL SEE TO IT!
Praise for the series:
'He's very good...my daughter monster-munched her way through it - and so did I' The Times
'I love that I can take my kids to meet their favourite authors and make books come so alive. Steve Cole a joy today. Thanks @edbookfest' Sarah Brown, wife of ex PM Gordon, 2012
'A cracking read for boys' The Sun
'In a fantastically improbable but sustained plot . . .This joke-filled story with zany illustrations is underpinned by a serious theme' Children's Book of the Week, The Sunday Times
'The humour is perfectly suited to a young boy… good books for boys are harder to find, and this one is truly exceptional' The Bookbag Orders
About the Author
Other Formats
Book Info
272 pages
Interest Age: From 8
Steve Cole
More books by Steve Cole
Author's Website
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Simon & Schuster Childrens Books an imprint of Simon & Schuster Ltd
Publication date
14th July 2016
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Most jobs are considered to be "at will" employment, but employees may still be able to sue an employer if the reason for termination is determined to be illegal. Unfortunately,… read more →
The U.S. Equal Pay Act of 1963 mandated that women and men in workplaces be paid equally for equal work. Despite this mandate, 53 years later women are still only… read more →
When incidents occur that affect the well being of employees or undermine the company culture, it is important to address the issues in a way that is effective and legal.… read more →
New rules about salary thresholds and overtime pay will go into effect on December 1, 2016. The new rules will replace the legislation that has been in effect since 2004… read more →
Massachusetts Governor Charlie Baker signed a bill into law on August 1, 2016 that has the potential to change the way that salaries are determined in a major way. The… read more →
In January of 2015, several organizations including NELP (National Employment Law Project) and All of Us or None took action to urge President Obama to issue an executive order to… read more →
The Patient Protection and Affordable Care Act received a fresh breath of life following the decision of the Supreme Court to uphold it. As a result, some major health care… read more →
Firing an employee can be very difficult, and it's almost always an awkward situation. For most managers, firing employees is the part of their jobs they dread the most. Fortunately,… read more →
Employers have to readjust and comply with more changes than ever. On top of the multitude of employment law changes, the continued economic stresses and now employers are scrambling to… read more →
If you're like many HR professionals you may have, at one time or another, found yourself questioning the job title of a position that was being created or one that… read more →


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oliver707 Review
Ann Loren Girls' 2-piece Damask Dots Set
I bought matching outfits for my two granddaughters. They are going to wear them to school on Grandparents Day. I have bought several AnnLoren outfits for the girls and this one may be my all time favorite.
- How did the color on site compare with the actual product? Perfect!
- Please tell us about the quality of the product. Excellent
- How accurate was the sizing? True to size
Was This Review Helpful?


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Politics Technology
Snowflakes: Troll Targets Say Twitter's New Filters Don't Go Far Enough
TWITTER HAS HEARD the complaints about abuse on its platform—really, it has. It's heard them so loud and clear that it's rolling out new ways to help you shut those voices out.
At least, that's the message Twitter sought to send yesterday, when it announced two new tools that give users more ways to control what tweets they see. The first lets you limit who gets to buzz your phone with @-replies only to people you follow. The second is a so-called "quality filter," which sounds like a step toward finally curbing the mob-style harassment Twitter seems so optimized to enable. But some targets of that harassment aren't so sure it's a meaningful fix. – READ MORE


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Meet the Watercolour Deathly Hallows Clematis Wristlet, she is a medium size clutch that can be used for a variety of occasions and purposes. The outer fabric features a beautiful pattern of layered watercolours flowing into and out of the Deathly Hallows symbol for this HP lovers clutch. It also features navy blue vinyl for that extra bit of sophistication. The inside is just as beautiful, with another watercolour fabric!
To create her I used a combination of colors and patterns to create a wristlet that is unique and shiny for fans of fantasy and magic. She is made from high quality cotton fabric printed from Spoonflower and designed by my collaborator Sarah.
To give her shape and stability I used a woven interfacing on her interior and fusible fleece on the exterior pieces. She has a zipper closure and a detachable strap. She is made using a Blue Calla pattern.
BAG DIMENSIONS & FEATURESThe dimensions for the body of the bag are:
- 10 inches wide
- 5.5 inches tall
- Her detachable strap is made with a D-ring attached to the bag and a lobster claw clasp on the strap.
- The strap measures 5.5 inches long and 1 inch wide.
- She has a cute interior pocket.
Clematis is a versatile wristlet. She can be used for the following occasions and purposes:
- Evening bag to carry your essentials on a night out.
- Keep her inside your work bag for dinners and events after work.
- Remove the strap to use as a make up or medication bag.
- Make-up bag.
- Carry potions and other magic nick-knacks.
- Perfect bridesmaid gift. Order a matching set for your bridal party or individual bags.
Due to the interfacing, vinyl and the hardware used in Clematis, it is only recommended to spot clean her.
- In stock items will ship in 1-3 business days.
- Otherwise, this item is custom ordered and usually takes approx. 1-2 weeks creation time.
- Please note: Should you have a particular timeline in mind, or if this item is a gift, please message me to let me know or visit our FAQ's for more info.
This item also comes in a darker, more graphic black and white variation, which you can find HERE!
Do you like the shape of Clematis but would like to see her in different colors or patterns?
Please get in touch with me and tell me your dreams!


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Dec 29, 2016 Newsdesk Industry Talk, Latest News, Macau
A Hong Kong-based family company – idNerd Studio – is in talks with several casino operators in Macau and beyond with a view to supplying what it says is state-of-the-art display and animation technology for stadium-style casino electronic games.
The product – X Stadium – uses a high-quality graphics card and is said to be able to create attention-grabbing animated images for the large overhead displays typically found on casino stadium-style games. The product was exhibited at Macao Gaming Show 2016 in November.
"X Stadium will turn your otherwise expensive and lifeless stadium game display into exciting, entertaining, and eye catching promotional marketing tools," states the firm in its promotional material.
The animations are based on real-time data in the 'trends' found in game results. A search for patterns in baccarat results is of particular appeal to many Chinese casino gamblers.
"On a traditional stadium game you have a lot of TV displays that are all driven by a video matrix, where your video comes in, and then is fed to various screens: that rather restricts what you can do," explained idNerd's managing director, Peter Wong (pictured, right).
"Ours is a completely virtual display where we can move graphics and content anywhere on the screens," he added.
X Stadium is fully compatible with Macau-based gaming equipment supplier LT Game Ltd's stadium-style electronic casino games.
Mr Wong's company was introduced to LT Game by Peter Johns, of casino gaming consultancy Golden Panda International. Mr Johns was previously vice president of electronic games for Macau casino operator Galaxy Entertainment Group Ltd.
X Stadium, while only a media device and not a gaming device, can nonetheless be linked to an electronic card shoe used for an electronic table game. So for example, when six 'banker' results turn up in a row in baccarat; that will come up on the game's media displays, highlighting the fact.
Hong Kong-born Mr Wong grew up in Canada but returned to Hong Kong 13 years ago. His brother Jacky acts as creative director of the firm, and has previously worked on animations for media organisations including Cartoon Network in Canada.
Peter Wong said there are "quite a few operators in Macau that are interested already" in X Stadium, as well as operators in "other markets".
The firm hopes to have the product "up and running" in Macau venues by at the latest the second quarter of 2017.
"Our product is not a gaming device, it's a media device. What it's doing is taking our signage and your [the casino's] hardware, and making it more appealing, so we are creating more life for the casino," stated the entrepreneur.
Aug 23, 2017
Jul 26, 2017
Aug 23, 2017
Aug 23, 2017
Aug 01, 2017Sponsored content Aristocrat Leisure Ltd strives to lead the gaming industry in creating combinations of innovative cabinets and games that really entertain. Its game and cabinet creations are designed to help operators cater to all types of players and gaming floor segments. "Our global teams of...
Aug 23, 2017A huge tropical storm on Wednesday – with speeds of up to 165 kilometres per hour (103 mph) according to the local weather bureau – brought damage to some Macau casino resorts as well as flooding...
Aug 23, 2017
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Aug 11, 2017Macau's traditional gaming zone on downtown peninsula...
Jun 27, 2017Management at two major casino venues in the Philippine...
Nov 23, 2016Hong Kong-listed casino equipment supplier Paradise...
Oct 27, 2016As Studio City – the US$3.2-billion, 2015 addition to...
May 26, 2016Macau's pacapio Chinese lottery game will celebrate its...
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Number of dealers in Macau's gaming industry as of the second quarter of 2017


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Seunghan Song's Window phone is more form than function, it can change the appearance of its display to reflect weather conditions. "Window Phone", makes accurate predictions and even changes its display to reflect the climatic conditions outdoors.
The Window Phone is a concept mobile phone that is able to transform its appearance like a window. The phone is designed as a thin, clear and transparent plastic sheet, which remains clear during a sunny day, becomes humid during a rainy day and takes a dump outlook during a snowy day.
The user can write text messages or draw pictures on the phone in different weather conditions which will later transform and show as SMS characters. The screen features sensitive interaction with the user and comprises the state of a window in different weather conditions.
Designer: Seunghan Song


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A visible record of history along the High Street
This Activity, which is specifically supported by our partner, the Skelton & Brotton Parish Council, has an ambitious aim. It plans to bring together local historical expertise, local secondary school children, local craft skills and local businesses to illustrate history and assist tourism in the form of a Mosaic trail.
We intend to develop the trail through the village, incorporating businesses and premises of historical significance. We have worked with students at Freeborough Academy, Skelton Youth Club members and the Skelton History Group, together with property owners, to identify the appropriate buildings. Designs (samples shown below) have been created based on the history of the buildings involved.
Mosaics are now being developed with local artists and will be placed with the help of the school students. Some completed mosiacs, and work in progress on others, are shown below.


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If the inside of your top freezer has an accumulation of ice on the top, bottom or walls that is ¼ inch or more thick, it's time to defrost it. A buildup of frost and ice inside the freezer can prevent it from running efficiently and properly. Not only can this affect the quality of your frozen foods, it also can increase your energy bill since the freezer is working harder to run.
Things You'll Need
- Old towels
- Ice scraper
- 2 large pots
- 1 tbsp. baking soda
- Rags
Take all items out of the freezer, then unplug the appliance from the electrical source. Put the items into another freezer, whether it's one of a relative, friend, neighbor or your own. Keep in mind that a top freezer is usually connected to the same electrical source as the refrigerator, so you'll likely need to remove the items from the refrigerator as well and keep them cold with ice or some other means.
Lay down old towels around the floor where you are working. This will keep the mess to a minimum and make cleanup easier.
Scrape off as much ice as you can with an ice scraper. Discard the loosened ice into the sink.
Bring a large pot of water to a boil. Once the water is rapidly boiling, set it inside the freezer and close the door.
Leave the pot of water in the freezer for 10 to 15 minutes. While you're waiting, bring another large pot of water to a boil.
Remove the pot from inside the freezer and scrape off as much ice as possible with the ice scraper. Use towels to soak up any water from inside the freezer.
Put the fresh pot of boiling water in the freezer and shut the door. Wait 10 to 15 minutes, then scrape off the ice and soak up the water. Continue this process until all of the ice is out of the freezer.
Clean the inside of the freezer with a mixture of 1 quart of warm water and 1 tbsp. of baking soda. Wipe down the bottom, top and sides of the freezer with a rag and this mixture to get rid of any unpleasant odors. Go over the surface with a rag dampened with plain water, then dry the freezer thoroughly with a clean rag.
Plug the freezer back in. Wait 30 minutes for the freezer to get cool before replacing food items.
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