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- Praziquantel - 50mg
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Ingredients: Rice bran oil, fragrance oil.


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Most Haunted Ghost Hunters bonus (2006) [DVDRip (DivX)]
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Name:Most Haunted Ghost Hunters bonus (2006) [DVDRip (DivX)]
Total Size: 709.58 MB
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Last Updated: 2010-12-17 01:13:00 (Update Now)
Torrent added: 2009-09-12 00:37:22
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ghost_hunters-yvette.jpg (Size: 50.90 KB) (Files: 1)
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Announce URL: http://www.thebox.bz:2710/00000000000000000000000000000000/announce
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This torrent was uploaded from www.TheBox.bz (signup is open and free) - the home of all British TV
Ghost Hunters
Yvette Fielding and Ciaran O'Keeffe completed investigations for the Ghosthunters book, published in 2006. This was a limited bonus DVD for books sold through ASDA stores.
Sorry i cant remember the investigations.
possible investigations
Yvette and Ciaran have chosen five new cases never examined before and carried out their own investigations, in locations including a Cheshire family home, a deserted shipyard (most haunted investigated here afterwards) and an abandoned church - now used as a nightclub
Resolution : 608x336
Butrate : 1238
Codec : xvid
Bitrate 128
Some of the other rather spiffing shows available now and always if you signup free @ www.TheBox.bz:
Comedy: The Office, Peep Show, Coupling, Chef, Fawlty Towers, Only Fools and Horses, The IT Crowd, Brass Eye, Nathan Barley, Young Ones, Monty Python, Blackadder, Alan Partridge, Father Ted, Coupling, Little Britain, Mr Bean, Extras, Green Wing, Mighty Boosh
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Food: Hell's Kitchen, Jamie Oliver, The F Word, Kitchen Nightmares, Masterchef, Great British Menu, Gordon Ramsay's Cookalong
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Kids: Teletubbies, Blue Peter, Tikkabilla, Andy Pandy, Postman Pat, Shoebox Zoo, Brum, Bob The Builder, Young Dracula, Sarah Jane Adventures, Shaun The Sheep
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β-Catenin plays an important role in the regulation of vascular endothelial cell-cell adhesions and barrier function by linking the VE-cadherin junction complex to the cytoskeleton. The purpose of this study was to evaluate the effect of β-catenin and VE-cadherin interactions on endothelial permeability during inflammatory stimulation by histamine. We first assessed the ability of a β-catenin binding polypeptide known as inhibitor of β-catenin and T cell factor (ICAT) to compete β-catenin binding to VE-cadherin in vitro. We then overexpressed recombinant FLAG-ICAT in human umbilical vein endothelial cells (HUVECs) to study its impact on endothelial barrier function controlled by cell-cell adhesions. The binding of β-catenin to VE-cadherin was quantified before and after stimulation with histamine along with measurements of transendothelial electrical resistance (TER) and apparent permeability to albumin (Pa) under the same conditions. The results showed that ICAT bound to β-catenin and competitively inhibited binding of the VE-cadherin cytoplasmic domain to β-catenin in a concentration-dependent manner. Overexpression of FLAG-ICAT in endothelial cell monolayers did not affect their basal permeability properties, as indicated by unaltered TER and Pa; however, the magnitude and duration of histamine-induced decreases in TER were significantly augmented. Likewise, the increase in Pa in the presence of histamine was exacerbated. Overexpression of FLAG-ICAT also significantly decreased the level of β-catenin-associated VE-cadherin following histamine stimulation. Taken together, these data suggest that inflammatory agents like histamine cause a transient and reversible disruption of binding between β-catenin and VE-cadherin, during which endothelial permeability is elevated.
- endothelial barrier
- cell-cell junction
- signal transduction
- inflammation
the vascular endothelial barrier regulates the passage of fluid, solutes, and circulating cells from the blood into the surrounding tissues. The permeability property of this barrier is determined in part by the integrity of endothelial cell-cell adherens junctions. Disruption of the junctions can lead to microvascular leakage, a common and important consequence of inflammation seen in ischemia-reperfusion injury, trauma, and diabetic microvascular complications (28, 47–49). Many inflammatory stimuli have been shown to be capable of altering endothelial cell-cell adhesive interactions rendering leakage via the paracellular pathway (35, 39, 42, 46). The precise molecular mechanisms underlying the increased paracellular permeability in inflammation remain incompletely understood. Heterogeneous responses are observed among various tissue origins of endothelial cells under different types of stimulation.
Several structural and signaling molecules have been characterized for their roles in forming and regulating endothelial cell-cell junctions in the vascular wall. Among them, VE-cadherin, a transmembrane protein that comprises the adherens junction, is essential to the maintenance of microvascular barrier properties (7, 8, 18). The molecular basis underlying VE-cadherin-mediated cell-cell adhesion has been a subject of intense investigation (2, 9, 22, 28, 30, 41, 46), resulting in the identification of several catenins in the junction complex as important molecular linkage between VE-cadherin and the cytoskeleton. The β-isoform of catenin is a multifunctional protein that participates in both Wnt signaling and cell-cell adhesive interactions. In endothelial cells, β-catenin joins other catenins forming a junction complex anchored to the actin cytoskeleton, promoting cell-cell adhesions and maintaining barrier function (28, 45). We have previously shown that the expression of a recombinant VE-cadherin cytoplasmic domain construct, which competes with native VE-cadherin for β-catenin binding, increases the permeability of both endothelial cell monolayers and intact coronary venules (18).
While the aforementioned study demonstrates the importance of β-catenin/VE-cadherin binding in maintaining normal endothelial barrier function under nonstimulated conditions, other studies have identified changes in the adherens junction complex that occur in association with endothelial hyperpermeability elicited by inflammatory stimuli. For example, histamine, a typical edematogenic factor involved in acute inflammation, has been shown to increase tyrosine phosphorylation of VE-cadherin and β-catenin (3, 33). Likewise, systemic inflammation caused by thermal trauma produces a similar response in endothelial cells characteristic of junction disorganization and β-catenin redistribution (37–39). It is not clear, however, whether altered binding of VE-cadherin and β-catenin serves as a mechanism for the hyperpermeability response.
The purpose of this study was to test whether decreased binding between β-catenin and VE-cadherin contributed to histamine-induced endothelial hyperpermeability. We validated the construction and overexpression of the 9-kDa inhibitor of β-catenin and T cell factor (ICAT) as a tool to investigate the interaction between β-catenin and VE-cadherin with respect to its importance in maintaining junctional integrity. ICAT was chosen for its ability to competitively bind to free β-catenin (13, 14, 16, 36). Previous reports have indicated that ICAT overexpression does not irreversibly disrupt the β-catenin-dependent cadherin complex in cultured epithelial cells or Xenopus oocytes in vivo; however, ICAT overexpression apparently reduces E-cadherin-mediated cell-cell adhesions during periods of dynamic junctional rearrangement (14, 15). Intrigued by the findings in epithelial cells, we evaluated the role of ICAT overexpression on β-catenin-VE-cadherin binding dynamics and barrier function in human vascular endothelial cells.
Protein expression and purification.
Total RNA was extracted from human umbilical vein endothelial cells (HUVECs). The cDNA product spanning the coding region of ICAT mRNA was amplified using RT-PCR (5′-primer: 5′-AACCGCGAGGGAGCTCCCGGGA AGA-3′ and 3′-primer: 5′-TGCAGCTACTGCCTCCGGTCTTCCGTCTC-3′, based on the human ICAT mRNA sequence, GenBank Accession No. AB021262). The PCR product was cloned into the pQE-30UA vector (Qiagen, Valencia, CA), with the recombinant ICAT expressed as a 5′-terminal, 6× His-tagged fusion protein. Fresh culture of Esherichia coli harboring the plasmid pQE30/ICAT were incubated with LB broth containing ampicillin (100 μg/ml) at 37°C for 2 h. Isopropyl-β-d-thioglactoside (IPTG) was then added to the bacterial culture at 1 μM, followed by an incubation for an additional 4 h. The culture was harvested, and cell pellet was resuspended in B-PER Reagent (Pierce, Redford, IL) for lysis. The clarified supernatant was loaded into prebalanced nickel-nitrilotriacetic acid (Ni-NTA) spin columns (Qiagen). After a wash, ICAT was eluted in a buffer containing 250 mM imidazole, and the extract was dialyzed against 20 mM Tris·HCl-buffered saline (pH 7.5) for the removal of imidazole. The His-tagged VE-cadherin cytoplasmic domain (CPD) and GST-tagged β-catenin (residues 134–664) were individually expressed in E. coli and purified as previously described (15, 18).
Protein binding assays.
Recombinant ICAT protein immobilized on Ni-NTA agarose beads was incubated at 4°C for 4 h with HUVEC lysate. Beads were washed five times with 20 mM Tris·HCl-buffered saline (pH 7.5) containing 0.3% Triton X-100 and then boiled in a sample loading buffer. Eluted proteins were subjected to PAGE and Western blot analysis. After protein transfer, the polyvinylidene difluoride membrane (0.2 μm) was first blotted with a monoclonal antibody to the His tag (Qiagen) for the detection of His-tagged ICAT. Afterward, the membrane was stripped and reprobed with horseradish peroxidase-conjugated anti-β-catenin (BD Biosciences, Lexington, KY). For the competitive binding assay, GST-tagged β-catenin (residues 134–664) was immobilized on glutathione agarose beads (Pierce) by an incubation of the beads with β-catenin-expressing E. coli lysate. Binding assays were performed in 20 mM Tris·HCl buffer (pH 8.0) containing 100 mM KCl, 10 mM MgCl2, and 1 mM DTT. His-tagged VE-cadherin CPD and His-ICAT were sequentially added to GST-β-catenin-bound beads and incubated at 4°C for 2 h in a total volume of 100 μl. After centrifugation, the supernatant was removed, and beads were washed five times with wash buffer [20 mM Tris (pH 8.0), 20 mM KCl, 1 mM DTT, and 0.1% Triton X-100]. After the last wash step, beads were resuspended in 50 μl of gel loading buffer, and eluted proteins were analyzed using SDS-PAGE and Western blot analysis.
ICAT transfection.
HUVECs (Cambrex, Walkersville, MD) were grown and maintained in endothelial growth medium-2 (EGM-2; Cambrex). Cells were transfected with plasmid pFLAG-CMV2/ICAT (14) or empty vector (mock) with the Nucleofector II Device (Amaxa Biosystems, Cologne, Germany) according to the manufacturer's instructions. Briefly, HUVECs grown to 80–90% confluence in EGM-2 were trypsinized and washed with PBS. The number of cells was counted, the suspension was centrifuged at 100 g for 10 min, and the pellet was resuspended in HUVEC nucleofector solution (Amaxa Biosystems) at 5 × 106 cells/ml. Plasmid DNA (2 μg) was added to 100 μl of the cell suspension, and the mixture was transferred into a cuvette for nucleofection. Immediately after nucleofection, 500 μl of prewarmed EGM-2 were added to the cuvette, and, after a 15-min incubation at 37°C, cells were seeded into either 35- or 60-mm culture dishes. At 4–6 h posttransfection, cells were washed with PBS, and dishes were refilled with fresh medium. Cells were used for study at 2–3 days posttransfection.
Transendothelial electrical resistance.
The endothelial barrier property related to cell-cell adhesions was evaluated by measuring transendothelial electrical resistance (TER) as we previously described (5). Briefly, HUVECs were transfected with pFLAG/ICAT or empty vector and, at 48 h posttransfection, were subcultured onto ECIS electrode arrays (Applied Biophysics, Troy, NY) at 105 cell/cm2 and grown overnight at 37°C. With culture medium serving as the electrode, barrier function was dynamically measured by determining the electrical impedance of a cell-covered electrode. A 1-V, 4,000-Hz alternating current signal was supplied through a 1-MΩ resistor to approximate a constant-current source. The in-phase voltage (proportional to resistance) and out-of-phase voltage (proportional to capacitive resistance) were measured and analyzed with ECMS 1.0 software (CET, Coralville, IA). Endothelial barrier function was expressed as TER normalized to baseline. Only endothelial monolayers with a baseline TER of 5,000 Ω or higher were used for experiments in this study.
Endothelial monolayer permeability to albumin.
The transendothelial flux of albumin across cultured endothelial cell monolayers was measured using our previously described method (18, 39). Briefly, HUVECs were seeded at a density of 3 × 105 cells/cm2 on gelatin-coated Costar Transwell membranes (Corning, Corning, NY) and grown 2–3 days until confluent. Cells were washed with endothelial basal medium (EBM) supplemented with 0.5% FBS and incubated with the same medium for 1 h. Histamine (100 μM) or an equivalent volume of vehicle was added to the top (luminal) chamber, followed by the addition of FITC-labeled BSA (Sigma) at a concentration of 1.0 mg/ml. Plates were incubated at 37°C for 30 min, and samples were removed from both the top and bottom (abluminal) chambers for fluorometry analysis. The readings were converted to albumin concentrations with the use of a standard curve. The sample concentrations were then used in the following equation to determine the apparent permeability coefficient of albumin (Pa): Pa = [A]/t × 1/A × V/[L], where [A] is the abluminal concentration, t is time (in s), A is the area of the membrane (in cm2), V is the volume of the abluminal chamber, and [L] is the luminal concentration.
Immunoprecipitation and Western blot analysis.
HUVECs were grown and maintained routinely in gelatin-coated dishes containing EGM-2. Medium was changed to EBM (Cambrex) supplemented with 0.5% FBS for 4 h before the collection of lysates. Cells were lysed in a Tris·HCl lysis buffer containing 1% Triton X-100 and protease inhibitors. The lysate was clarified by centrifugation at 13,000 g for 20 min, and supernatants were incubated for 4 h with various antibodies preadsorbed onto protein A/G-coupled agarose beads (Santa Cruz Biotechnology) for pull down of respective proteins. Proteins eluted from beads or in total lysates were separated by SDS-PAGE, transferred to nitrocellulose or polyvinylidene difluoride membranes, and blotted with monoclonal antibodies to β-catenin, VE-cadherin, or the His tag. The membrane was incubated with the corresponding horseradish peroxidase-conjugated secondary antibody, and immunoreactive bands were detected using the Pico Supersignal chemiluminescent substrate (Pierce). Images of the blots were acquired by reflectance scanning densitometry, and band intensity was quantified using National Institutes of Health Image software.
Immunofluorescence microscopy.
HUVECs grown on gelatin-coated coverslips were fixed with 3% paraformadehyde and permeabilized with 0.2% Triton X-100. For the labeling of recombinant ICAT, cells were incubated with a monoclonal anti-FLAG antibody (Sigma) for 1.5 h, followed by an incubation with FITC-labeled anti-mouse IgG (Santa Cruz Biotechnology). Coverslips were then mounted onto slides for fluorescence microscopy and viewed with an Axiovert 200M fluorescent microscope equipped with an AxioCam MRm camera (Carl Zeiss, Thornwood, NY). Digital images were collected with Zeiss Axiovision 4.0 software.
Data analyses.
For all experiments, n is given as the number of dishes or wells of endothelial cells studied. Data are expressed as means ± SE. Statistical analysis with ANOVA followed by Bonferroni t-tests was performed to evaluate the significance of intergroup differences. Significance was accepted at P < 0.05.
ICAT expression and binding interactions with β-catenin in vitro.
The molecular structure of ICAT and its interaction with β-catenin are shown in Fig. 1. β-Catenin uses the same armadillo (arm) repeat domain to bind to several different intracellular ligands, including ICAT (Fig. 1A). Sequence alignment revealed that, like other members of the cadherin family, VE-cadherin shares with ICAT the conserved and critical amino acid residues involved in binding interactions with β-catenin (Fig. 1B), and ICAT binds to β-catenin arm repeats 5–12 in a manner similar to that of cadherins (10, 15). To demonstrate that ICAT binds β-catenin, recombinant ICAT was expressed and purified as a 9.6-kDa NH2-terminal, His-tagged fusion protein. This recombinant protein reacted with both anti-His monoclonal antibody and polyclonal antiserum against ICAT (Fig. 1C). Recombinant ICAT was able to bind to β-catenin from endothelial cells in a concentration-related pattern (Fig. 1D). Under the same conditions, blank Ni-NTA agarose beads did not show the adsorption of detectable β-catenin, corroborating the specificity of His-ICAT and β-catenin binding interactions. Importantly, by using in vitro competition binding assay (Fig. 2) in which GST-β-catenin-coupled glutathione agarose beads were incubated with the recombinant VE-cadherin CPD in the presence of different amounts of His-tagged ICAT, we found that the level of VE-cadherin bound to β-catenin beads decreased as a function of increased concentration of ICAT, confirming that ICAT effectively inhibited the binding of β-catenin to VE-cadherin in a concentration-related manner (Fig. 2).
Endothelial junction protein expression during ICAT overexpression.
To determine the potential impact of ICAT on endothelial cell-cell adhesions, FLAG-ICAT protein was overexpressed in HUVECs. As shown in Fig. 3A, transfection of pFLAG/ICAT with the aid of Nucleofector II yielded a high transfection efficiency in HUVECs without damaging cell viability and function. Transfection at 2 μg produced an optimal, high level of ICAT expression; thus, this concentration was used in subsequent experiments. Further analyses in fractionated cell lysates revealed a preferential distribution of the transfected ICAT in the cytosolic pool, with relatively less amount of protein detected in the nuclear extract and essentially none in the cell membrane (Fig. 3B), confirming the intracellular location of ICAT, consistent with a previous finding with epithelial cells. Interestingly, overexpression of recombinant ICAT did not significantly alter expression levels of VE-cadherin and β-catenin in endothelial cells (Fig. 3C).
Endothelial barrier function during ICAT overexpression.
To determine whether ICAT expression affects endothelial barrier function, we first assessed the TER of HUVECs transfected with ICAT. During the attachment of cells to the electrodes and subsequent monolayer formation, we observed no differences in the basal TER between FLAG-ICAT-transfected and mock-transfected HUVECs (data not shown). On the other hand, cells overexpressing ICAT displayed a greater response to histamine (Fig. 4), as both the magnitude (Fig. 4B) and duration (Fig. 4C) of the decrease in TER were significantly augmented in pFLAG-ICAT-transfected cells compared with nontransfected and mock-transfected cells, indicating that ICAT delays the recovery of histamine-induced barrier dysfunction (see Supplemental Table 1 for electrical resistance values).1
We then further examined the ICAT effect on endothelial permeability to protein. We found that the overexpression of ICAT did not affect basal Pa, as there were no significant differences in Pa between ICAT-transfected and mock-transfected cells. In contrast, HUVECs overexpressing ICAT displayed a significantly greater hyperpermeability response to histamine than mock-transfected cells (Fig. 5), consistent with the augmented decrease in electrical resistance (Fig. 4).
β-Catenin/VE-cadherin binding during ICAT overexpression.
Immunoprecipitation and Western blot analysis revealed no difference in β-catenin-bound VE-cadherin levels between mock- and ICAT-transfected endothelial cells under the basal, nonstimulated conditions (Fig. 6). After 2–5 min of histamine treatment, however, both ICAT- and mock-transfected cells showed significant decreases in the pool of β-catenin-bound VE-cadherin, indicating dissociation of the two molecules. The response, although not statistically significant, appeared to be more severe in cells overexpressing ICAT. Interestingly, at 10 min after histamine treatment, there was a sustained decrease in the level of β-catenin-associated VE-cadherin in ICAT-expressing cells, whereas mock-transfected cells showed reassociation of β-catenin and VE-cadherin. At later time points (30 or 60 min) of treatment, the fractions of β-catenin-associated VE-cadherin in both groups of cells returned to pretreatment levels (Fig. 6).
In this study, we present evidence suggesting a dynamic response of the endothelial adherens junction to acute inflammatory stimulation, in that dissociation of the VE-cadherin/β-catenin complex augments the extent, and delays the recovery, of histamine-induced hyperpermeability. This notion is supported by data showing that overexpression of ICAT, which competed with VE-cadherin to bind β-catenin, did not affect basal barrier function but increased the magnitude as well as the duration of the hyperpermeability response to histamine in endothelial cells. Moreover, we found that histamine caused a time-dependent dissociation of β-catenin from VE-cadherin concomitant with elevated permeability, which was enhanced with the overexpression of ICAT. Combined with our previous finding that experimental disruption of the adherens junction elevates microvascular permeability (18, 39), these results suggest that certain inflammatory stimuli may increase endothelial permeability through a mechanism involving the dissociation of β-catenin and VE-cadherin from the adherens junction. We have not yet tested whether the ICAT-induced exacerbation of barrier dysfunction is universal or histamine specific. We speculate that ICAT may affect the endothelial permeability response to other agonists in which the mechanism predominantly involves changes in adherens junctions.
Endothelial junctions are dynamically regulated by physical forces, cellular factors, and chemical mediators (17, 24). We and others have previously demonstrated that inflammatory mediators, including histamine, thrombin, growth factors, cytokines, oxidants, and activated neutrophils, can increase the transendothelial flux of fluids, macromolecules, and circulating cells across the microvascular wall, mainly via a paracellular route (3, 11, 23, 25, 27, 34, 49). Alterations in endothelial junction morphology and intercellular gap formation are typically observed concomitantly with fluid or macromolecule leakage. The homotypic binding of VE-cadherin molecules on adjacent endothelial cells is critical for the maintenance of intercellular junctions, and interfering with this binding by depleting extracellular calcium or introducing VE-cadherin monoclonal antibodies disrupts the junctions and elevates paracellular permeability (1, 7, 8, 12). While such dramatic perturbation of the junctional structures may not reflect the pathophysiology of endothelial cells in inflammation, alternative mechanisms involving a more subtle and dynamic response in the junction have begun to be appreciated. Within this context, the CPD of VE-cadherin represents an important target for dynamic modification by the intracellular signaling cascades triggered upon inflammatory stimulation. We have previously shown the importance of the VE-cadherin CPD in maintaining the structural integrity of junctional complexes, as interrupting the normal binding between VE-cadherin and the cytoskeleton by the introduction of an ectopic VE-cadherin CPD fusion protein caused increased permeability in endothelial cell monolayers as well as in intact microvessels (18). The results from the present study build upon the concept that the dynamics of VE-cadherin and β-catenin binding interactions regulate the junctional integrity and thus barrier function in endothelial cells, suggesting that VE-cadherin/β-catenin dissociation and reassociation, respectively, serve as a mechanism for the initial increase in endothelial permeability and subsequent restoration of normal barrier function after stimulation by acute inflammatory mediators.
An interesting aspect of our results lies in the finding that ICAT is able to compete with VE-cadherin for the β-catenin binding site in vascular endothelial cells. ICAT is a newly identified protein capable of blocking the binding between C-cadherin or E-cadherin and β-catenin in epithelial cells (14, 15). Crystal structure analysis indicates that the ICAT 3-helix bundle binds to arm repeats 10–12 of β-catenin and that the COOH-terminal extended domain of ICAT overlaps with, and essentially obstructs, arm repeats 5–9, which contain the binding regions of T cell factor, adenomatous polyposis coli (APC), and E-cadherin (10, 15). The sequence of the VE-cadherin CPD shares several highly conserved residues at critical positions in the β-catenin binding region (corresponding to the COOH-terminal tail of ICAT), and our in vitro binding assay confirmed that ICAT can compete with VE-cadherin for β-catenin binding in a concentration-dependent manner. Thus, in this study, we used ICAT primarily to inhibit the β-catenin binding to VE-cadherin. It is worth noting that this treatment may also cause inhibition of β-catenin's ability to activate T cell factor/lymphocyte enhancer factor (LEF). In endothelial cells, T cell factor/LEF activation promotes the expression of genes associated with angiogenesis (26). Therefore, prolonged overexpression of ICAT may inhibit endothelial cell proliferation or migration. Also, we did not examine whether ICAT altered VE-cadherin binding to other catenins, such as plakoglobin, which have been shown to be key elements in the assembly of intercellular junctions and thus modulate barrier function (32, 40). We expect that interference with plakoglobin binding to VE-cadherin would promote a similar effect as disruption of VE-cadherin/β-catenin binding.
Although the present observations were limited to a relatively short term, overexpression of ICAT may exert permeability effects over longer periods of time in the absence of inflammatory stimuli. This possibility is supported by the finding that adherens junctions are constantly undergoing remodeling with VE-cadherin turnover (40). However, it is unlikely that ICAT affects the de novo formation of adherens junctions in the same way as reannealing of disrupted junctions following an inflammatory stimulus, as we did not detect any apparent change in VE-cadherin and β-catenin levels in ICAT-overexpressing cells, and these cells produced electrical resistance and solute permeability comparable with those of mock-treated cells in the absence of inflammatory stimulation. Thus, ICAT may not significantly affect VE-cadherin turnover or disrupt junctional integrity in the absence of inflammatory stimulation. This was not surprising, considering that an injection of ICAT mRNA or protein into Xenopus embryos did not disrupt cell-cell adhesion or block the binding of C-cadherin to β-catenin during development (15) and that Madin-Darby canine kidney (MDCK) epithelial cells stably expressing epitope-tagged ICAT do not show evident changes in cadherin-based cell adhesion (14). On the other hand, posttranslational modifications of cadherins could potentially alter the ability of ICAT to competitively bind to β-catenin. For example, phosphorylation of serine residues in the common sequence motif SLSSL in both E-cadherin and APC tremendously increases their binding affinity for β-catenin (6, 21). Moreover, both E-cadherin and VE-cadherin form tight complexes with β-catenin once they appear in the endoplasmic reticulum, and these complexes move as individual units to the surface of respective cells for establishing cell adhesion (28). Additional factors to consider are how the formation of multiprotein complexes containing VE-cadherin and β-catenin affects their binding to each other as well as localization at specific subcellular compartments.
Although we saw no noticeable changes in cells overexpressing ICAT under basal conditions, after histamine stimulation these cells displayed an enhanced and prolonged hyperpermeability response in association with VE-cadherin/β-catenin complex dissociation. This pattern was also previously observed in MDCK cells expressing ICAT, with no changes during nonstimulated conditions but an enhanced cell scattering upon stimulation with hepatocyte growth factor, suggesting the involvement of ICAT in dynamic rather than steady-state cell adhesions (14). Histamine, upon binding to its receptors on endothelial cells, triggers several signal transduction cascades including activation of PLC and PKC, intracellular calcium mobilization, nitric oxide production, activation of MAPKs, myosin light chain phosphorylation and actin-myosin contraction, and tyrosine phosphorylation of junctional proteins (3, 4, 20, 29, 43, 50); all of these reactions contribute to the hyperpermeability response. Of particular note, tyrosine phosphorylation of β-catenin decreases its affinity for cadherins, resulting in the dissociation of β-catenin from the cadherin complex and weakened cell-cell adhesions (19, 28, 31). Based on these findings, we speculate that histamine promotes conditions that allow ICAT to capture β-catenin dissociated from VE- cadherin, slowing the reassembly of β-catenin/VE-cadherin complexes and thus the recovery of hyperpermeability.
Further analyses of the pattern of electrical barrier responses shown in Fig. 4 may provide some mechanistic insight regarding histamine-induced hyperpermeability. Clearly, ICAT overexpression enhanced the initial drop of TER upon histamine treatment, indicative of exacerbated barrier dysfunction. Considering that the recovery process in ICAT-expressing cells started at a lower set point, one may reason that the slower recovery might be due to the initial drop in TER. In other words, the rate of recovery may decrease as a function of the magnitude of loss of barrier function. While this is plausible, the Western blot data (Fig. 6) showed a delayed reassociation of VE-cadherin with β-catenin in a time course correlating with that of the TER response in ICAT-expressing cells, indicating an effect of prolonged VE-cadherin/β-catenin dissociation to cause delayed barrier recovery. On the other hand, an interesting phenomenon seen in mock-transfected cells is that, following the initial drop in TER after histamine, the barrier function not only quickly returned to the basal level but further increased (Fig. 4), which appeared to occur without an associated increase in VE-cadherin/β-catenin binding (Fig. 6). This suggests that different mechanisms may be involved in the barrier restoration process after inflammatory stimulation. One explanation would be that adherens junction dissociation triggers multiple recovery signaling events leading to an enhanced barrier defense. Another possibility is that other intercellular adhesion molecules, such as the tight junction, contribute to the recovery process where reassociation of VE-cadherin and β-catenin may further signal the reorganization of these cell-cell adhesive structures.
In summary, we show that histamine alters the binding interaction between VE-cadherin and β-catenin in association with hyperpermeability in endothelial cells. Overexpression of ICAT enhances and prolongs histamine-induced VE-cadherin-β-catenin complex dissociation and the hyperpermeability response. These results suggest that VE-cadherin and β-catenin binding dynamics are important determinants in microvascular barrier regulation.
This study was supported by National Heart, Lung, and Blood Institute Grants HL-061507, HL-070752, HL-073324, and HL-076079.
↵1 Supplemental data for this article is available online at the American Journal of Physiology-Cell Physiology website.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2008 the American Physiological Society


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Ford Offers Siri Eyes-Free Update to Older Vehicles for Free
Ford is offering Apple Siri Eyes-Free to millions of its older vehicles globally through a SYNC software update.
The new software update will be available to over five-million SYNC-equipped vehicles that date back to the 2011 model year.
The technology lets drivers activate Siri with a long press of the voice recognition button on the steering wheel, without having to take their eyes off the road. From there, drivers can ask Siri to perform numerous tasks such as making a phone call, looking up a phone number to a restaurant or other points of interest, setting a reminder or alarm, reporting on the weather, sending text messages as well as getting directions through Apple Maps.
SEE ALSO: Ford SYNC 3 Review: It Doesn't Suck Any More
The software update is available to owners of vehicles with the second generation of SYNC, known as MyFord Touch in North America.
"SYNC, Ford's entertainment and communications system, was designed to be flexible and updatable, just like other mobile technologies, so our customers are able to get the most out of their smartphones while behind the wheel," said Sherif Marakby, director, Ford Electronics and Electrical Systems Engineering. "Siri Eyes-Free is another great voice-activated feature that not only adds convenience but helps our customers keep their eyes on the road and hands on the wheel."
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What is Jeopardy?
Visit the Jeopardy TV game show official website to learn more on how to become a contestant and you'll discover if you're Canadian you can't. Is it because we're just too smart?
The issue seems to be our strict Canadian privacy laws and the ability to share information over the internet. I'm sure this is temporary after all Alex Trebek is one of us, born in Sudbury, Ontario.
Until this is rectified you'll just have to play along at home or try to win with Q107's Grand I.Q. test weekdays at 8:00am with Derringer in the Morning and 4:40pm on the Mo Show.


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Ms Heart Print
Ms Heart. Beautifully bold. Thinks first. Heart follows. Peony lover.
Our minimalist prints are made with love from original watercolour paintings. Bringing imagination and creativity to your home.
Wall art prints are printed on premium matte smooth 230gsm paper.
All our prints come frame less though all sizes can fit easily into Ikea Marietorp, Mossebo or Ribba frames.
Image 2 Credit: Georgina @woolandwillow
We Also Recommend


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Party Down
Episode 33: The Case of the Pastry Pervert
We review evidence regarding Sean's near miss with a Devil's Threesome, talk about David's weekend movie marathon, and games!
Posted in Podcast by David. Read More
Episode 32: DJ Poopie Teat
We talk about Drive, tons of sandwiches, a TV show grab-bag, The Grey, a couple iOS detective games, and Sean's Trial in Asif's Court next week.


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If a guest decides to reduce the number of nights during the course of the stay, they will be liable for a charge of 100% of the booking.
By proceeding with payment for this booking, I confirm that I have read and accept the SA-Venues.com terms of use.
Suedafrika-Reise.net ist der ideale Online-Reiseführer, mit dem Sie Ihren Urlaub in Südafrika planen und zugleich buchen können. Besuchen Sie uns auf Südafrika Reise


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Halkidiki Excursions & Tours
All excursions
Bully Minivan Excursion
Available until 30 September 2017 then from 1 May 2018
Halkidiki Excursions & Tours
: For those who want to get to know Halkidiki from a different point of view, the answer is hidden to this marvelous excursion!!!
Explore Halkidiki's breathtaking nature, beaches and villages in a group up to 6 persons, with a nostalgic way, with a VW T2 (1977), which you will have the chance to drive yourselves!! Don't miss the chance to visit Afitos, the most beautiful village of Kassandra peninsula, observe the birds at the lake of Sani, have a traditional greek pick-nick with olives, tzatziki, feta and dolmadakia at Fourka and swim into the amazing waters of Glarokavos!!!
Visit the tower in Nea Fokea, the church in Agios Pavlos, the lighthouse of Posidi and the Church of Panagia Faneromeni in Skioni. Walk at the square of Kriopigi and enjoy your coffee at the picturesque square of Agia Paraskevi, all while being informed about all the important facts of the area!
Meeting/pick-up point: Afitos area, on the main road Bus stop there will be parked the minivan.
Duration: 10:30am.
Start/opening time: 6:30pm.
Languages: English and German.
Included: Fuel, Snacks, picnic.
Not Included: Coffe in Agia Paraskeviv.
Availability and price
Cast Away
Available until 24 November 2017 then from 4 May 2018
Halkidiki Excursions & Tours
: What better way to spend the day than sailing over the Aegean, stopping at beaches and islands along the way? That's the score on this cruise to Sithonia, the middle of Halkidiki's peninsulas. First up, you'll board a galleon-style ship, lazing on deck as it cuts through the waters and passes by green islands. Top up your tan or join in the games organised by the friendly crew. Then, you'll drop anchor at Toroni Bay, where a stretch of sand melts into shallow waters. Spend a couple of hours here soaking up the sun and swimming in the sea before rejoining your ship for lunch and a glass of wine. Next, your boat will cruise on to Turtle Island, a pine-wooded island that's shaped like a sleeping turtle. Here, you'll get another chance to swim or snorkel or lounge on deck before the crew call time up and you sail back to port.
Meeting/pick-up point: At the htoel.
Start/opening time: Between 8.30-10am.
End/closing time: Between 5-6.30pm.
Availability and price
Fishing Experience
Available until 30 September 2017 then from 1 May 2018
Halkidiki Excursions & Tours
: Until now fishing in distant and hard to reach fishing grounds for those who did not have a boat, was not possible.
Experienced staff, will welcome you to the sea world and the sport fishing experience in order to guide you and take you to fishing spots in the Aegean sea. Enjoy with us the emotion and Fishing experience. Our experience and love for the sea is your warranty for a memorable time. The ship "Angel Archangel" fully equipped and "Fishing ready" in compliance with all sea safety rules will take you safely and securely to the fishing spots you desire, coastal, midsea, in inslets and islands of the Aegean. Enjoy with your friends the sea adventure by choosing a Fishing excursion or "Create" an excursion specialy for you, and let the fishing games begin!
After fishing, we are sailing straight to Nefeli Restaurant at Polichrono Halkidiki (a sea view resaturant) for having a meal with your fresh caught fishes. That includes: Starter, Salad, wine, water and all fishes will be cooked fried or grilled.
Duration: 8hours.
Start/opening time: At 9am.
End/closing time: At 5pm.
Min. Age: 15 years.
Languages: English.
Included: Fuel, boat, fishing equipment, dinner, snacks, and drinks.
Not included: Transfer.
Availability and price
Mount Athos Cruise
Available until 29 October 2017 then from 2 May 2018
Halkidiki Excursions & Tours
: Mount Athos is up there alongside the Acropolis in the Greek landmark stakes. You see, it's the world's one and only monastic republic. It's made up of 20 cliff-hugging monasteries, some of which date back to the ninth century.
Some 1,700 Orthodox monks live here, dressed in their signature black robes and pillar box hats. Access to Mount Athos is extremely limited. What's more women are forbidden from going within 500 metres of the place. But that's where this trip comes in. Your cruise boat sails close to the shores of the Athos Peninsula so you can take a sneaky peek at this holy site.
Start/opening time: At 9am.
End/closing time: At 5pm.
Availability and price
Rent a Boat
Available until 30 September 2017 then from 1 May 2018
Halkidiki Excursions & Tours
: We offers speedboats for hire, and this is a great opportunity for YOU, your friend and family, to add variety together with an unforgettable excitement on your vacations. We operate on high standards in all stages of a speedboat rental offering quality services so our clients experience a once in a life time vacation.
Our company offers you the opportunity to visit some or the wonders of Halkidiki which are accessible only by boat, visits in places not easy to describe but easy to capture through photos and videos, (e. g. the island of Kelyfos) while swimming in the crystal clear waters of its famous blue sea, in private, is a unique, once in a life time, experience! Our staff are professional instructors with great experience and knowhow on speedboats which are responsible for your training for driving and handling the speedboat, before your departure, informing you properly about the sea regulations and the use of safety equipment, in case of an emergency situation, on board.
Duration: 10 hours.
Start/opening time: At 9am.
End/closing time: At 7pm.
Min. Age: Adults from 18 years old can drive the boat.
Others: No license is required for handling a motor boat under 30hp.
Availability and price
Restaurant Thea Thalassa
Available until 30 September 2017 then from 1 April 2018
Halkidiki Excursions & Tours
: Afitos or Athitos? That is the usual question one has to answer when asked for the name of this rock-built village, located in Chalkidiki. Both names are in fact valid, since Afitos is the modern name of the ancient village Afitis. The beautiful little streets with the stone houses are full of secrets and surprises.
The blue waters of the sea can be admired on the balcony of the village, where also the tavern, Thea Thalassa ('sea view'), is located. Taste good, traditional Greek cuisine in a modern environment and enjoy the magnificent view.
Times: Open daily from midday.
Option 1: Cold appetisers (tzatziki, aubergine, cheese salad), Greek salad, fried calamari, fried courgette, traditional midopilafo (boiled rice with muscles), octopus with vinegar, bread, fruit or sweet, 0,5lt red or white wine. All for two diners.
Option 2: Warm appetiser fired courgette balls, Greek salad, fried shrimps, grilled sardines, fried anchovy, bread, fruit or sweet, 0,5lt red or white wine. All for two diners.
Availability and price
Thessaloniki Shops and wine
Available from 7 May 2018
Halkidiki Excursions & Tours
: Thessaloniki is also available as a shopping trip only, combined with winery visit. Go shopping in Thessaloniki, the second largest city in Greece, in a wide range of well-known shops including Marks & Spencer, The Body Shop and lots of designer boutiques. After all the shopping, take a break and have a refreshing drink in one of the chic waterfront café-bars.
Meeting/pick-up point: Depends on clients' hotel.
Start/opening time: At 7.20am.
End/closing time: At 3.45pm.
Availability and price
Need an airport transfer?
Transfers from Halkidiki
Sithonia - Porto Carras Grand Resort
Sithonia - Neos Marmaras, Halkidiki
Eagles Palace
Athos - Ouranoupolis, Halkidiki
Kassandra - Nea Kallikratia, Halkidiki
Potidea Golden Beach
Nea Potidea, Halkidiki
Petrino Suites
Kassandra - Afitos, Halkidiki
Renaissance Hanioti Resort
Kassandra - Hanioti, Halkidiki
Sousouras Hotel
Kassandra - Hanioti, Halkidiki
Anna Maria Paradise
Kassandra - Pefkohori, Halkidiki
Pashos Hotel
Kassandra - Kriopigi, Halkidiki
Georgalas Sun Beach Hotel
Kassandra - Nea Kallikratia, Halkidiki
Royal House Kallithea
Kassandra - Kallithea, Halkidiki
The White Villa At Sani
Kassandra - Sani Beach, Halkidiki
Villas Kosma Bomo Club
Kassandra - Hanioti, Halkidiki


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Dan Myers
They really don't make restaurants like Patina Restaurant Group's Brasserie 8 1/2 anymore, especially in New York City. There's a lot of "wow" factor as you walk through the large doors of the Solow Building and check in with two hosts, one upstairs and one after you've descended down an eye-catching spiral staircase and into a red-carpeted lounge area with original works by Matisse and Léger on the wall. The massive dining room is just beyond that, with plenty of white tablecloths and seating for several hundred, as well as another bar.
It's a massive restaurant with a traditional French brasserie menu, and during a recent meal there I couldn't help but think that I'd been transported 17 years into the past, into those heady first days of the new millennium, for better or worse. The restaurant itself doesn't seem to mind not changing much at all in nearly 20 years; the website still proudly brags that it was "seen on Sex and the City."
Dan Myers
The wine list is surprisingly small, with only 26 reds and 25 whites available, but our Oregon pinot from Sharecropper's didn't warrant any complaints. Service was professional if slightly aloof; when a glass of wine spilled, our server awkwardly watched as we attempted to mop it up (the tablecloth was soon replaced.
The crowd appeared to be an even mix of older regulars, tourists, and large groups of coworkers. Even though the space is absolutely gorgeous, making the most of the basement location (it's a jewel in Patina's crown), I got the impression that Brasserie 8 1/2 is resting on its laurels, and not quite living up to its potential. This location could easily be home to a flagship restaurant for a chef like Ducasse or Robuchon, and its ideal location on 57th Street is enviable. (To get an idea of its potential, look no further than Gabriel Kreuther, the ground-floor restaurant in Solow's 42nd Street sister building, Grace, easily one of the best restaurants to open in the city in the past 5 years.) With a little imagination, Brasserie 8 1/2 could easily become a fine dining destination once again.


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I'm running a giveaway for the hardcover version of my book. Go here to enter to win!
And if you're looking for a deal on an ebook to give to your readerly friends for Christmas (or for yourself), it's on sale on Smashwords for $.99 from Black Friday through Cyber Monday, after which it will return to its regular price of $2.99.
Have a great Friday!