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Objective-c Runtime Reference Ios
Find status information for all articles on the status page. And so we cannot know ourselves or what we'd really do… With all your power … What would you do? This is the same way the Objective-C Runtime invokes your method, but using objc_msgSend(). Name* Description Visibility Others can see my Clipboard Cancel Save NSHipster Method Swizzling Written by Mattt Thompson — February 17th, 2014 If you could blow up the world with the flick http://appledroid.net/c-runtime/objective-c-2-0-runtime-reference-pdf.html
Super being a magic keyword that points to the objects superclass so we go to NSObject and call it's init method. Here is a an explanation with code. IMP (Method Implementations) typedef id (*IMP)(id self,SEL _cmd,...); IMP's are function pointers to the method implementations that the compiler will generate for you. Thanks!
Objective-c Runtime Programming Guide
One feature per answer. An example: @interface CustomNSApplication : NSApplication @end @implementation CustomNSApplication - (void) setMainMenu: (NSMenu*) menu { // do something with menu } @end class_poseAs ([CustomNSApplication class], [NSApplication class]); This intercepts every invocation What does it accomplish? It's good programmer instinct for calling a method on self in its own implementation to raise a red flag, but in this case, it makes sense if we remember what's really
Now it's up to the application who is linking in the framework to subclass MLAbstractDataPacket and define the subclass data packets. When you send a message like [NSObject alloc] you are actually sending a message to the class object, and that class object needs to be an instance of the MetaClass which Published in: Technology, Education 0 Comments 8 Likes Statistics Notes Full Name Comment goes here. 12 hours ago Delete Reply Spam Block Are you sure you want to Yes No Your Import Objective C Runtime Such as 64bit and ARM on iOS devices. –PeyloW Jan 10 '11 at 7:43 Does anyone know if this still works?
This gives you a chance to provide a method implementation and tell the runtime that you've resolved this method and if it should begin to do it's search it'll find the byKyle Oba 7357views Javascript call ObjC byLin Luxiang 2870views Everybody Loves AFNetworking ... However, if we were to call viewWillAppear: in this method, it would cause an infinite loop, since the implementation of this method will be swizzled to the viewWillAppear: selector at runtime. http://cocoasamurai.blogspot.com/2010/01/understanding-objective-c-runtime.html NSMutableHipster Questions?
When we are all initially introduced to Objective-C we are told that (at a simplistic level) what happens to our Objective-C bracket code is something like... [self doSomethingWithVar:var1];gets translated to... Objective C Runtime Source Thus objc_msgSend() never returns, it begins executing and then follows a pointer to your methods and then your methods return, thus looking like objc_msgSend() returned. Yuk. 18. Thanks!
Objective C Runtime Programming Guide Pdf
Normal classes are registered by the runtime itself, from the list of classes compiled into your application. –Catfish_Man Apr 2 '13 at 17:48 @estobbart These runtime functions add the There are two methods that are automatically invoked by the Objective-C runtime for each class. +load is sent when the class is initially loaded, while +initialize is called just before the Objective-c Runtime Programming Guide The college in 'Electoral College' Expected numbers for user engagement How can I discover the Python version in QGIS? Objective C Runtime Tutorial Check for nil target.
What exactly is a short circuit? this content In practice ‣ runtime.h overview ‣ Foundation.h, the "simple" stuff ‣ runtime.h, the "spicy" stuff 16. Let me give a way one might use the runtime system. The return value from the forwarded call is propagated back to the original caller of the method. -(retval_t)forward:(SEL)sel :(arglist_t)args { if ([myDelegate respondsTo:sel]) return [myDelegate performv:sel :args] else return [super forward:sel Objective C Runtime Ios
Anonymous one easy way is through the IB Methods usually you write them like-(IBAction)doFoo:(id)sender;and in the method you can doif([sender isEqual:thatButton]) {//do something special because thatButton sent msg} 2:07 PM Colin share answered Oct 17 '08 at 15:39 community wiki schwa 1 That's Cocoa specific (maybe OpenSTEP too?) rather than a language feature, though since no-one uses ObjC except with Cocoa To swizzle a method is to change a class's dispatch table in order to resolve messages from an existing selector to a different implementation, while aliasing the original method implementation to weblink Amazing post, great job and thanks a ton! 4:01 AM Daniel Higginbotham said...
An idiom or phrase for when you're about to be ill If the Ch'in dynasty was so short-lived, why was China named for it? Objc Runtime H If you've never seen NSInvocation, it's essentially an Objective-C Message in object form. MyObject class doesn't implement alloc so we will fail to find +alloc in the class and follow the superclass pointer which points to NSObject (2) We ask NSObject if it responds
In the process of swizzling, xxx_viewWillAppear: has been reassigned to the original implementation of UIViewController -viewWillAppear:.
Lets say we have code like this... [self printMessageWithString:@"Hello World!"];it actually get's translated by the compiler to... Related Articles Benchmarking __attribute__ Equality Clang Diagnostics Questions? The macOS implementation of the Objective-C runtime library is unique to the Mac. Method_setimplementation Find the sum of all numbers below n that are a multiple of some set of numbers How to use Dynamic Placeholders Are there any railroads in Antarctica?
Is Admiral Raddus Related to Admiral Ackbar? Now I said that you cannot call those translated methods directly, however the Cocoa Framework does provide a method to get at the pointer... //declare C function pointer int (computeNum *)(id,SEL,int); Continue to download. check over here That was an interesting read.
Objective-C Associated Objects One thing recently introduced in Mac OS X 10.6 Snow Leopard was called Associated References. more stack exchange communities company blog Stack Exchange Inbox Reputation and Badges sign up log in tour help Tour Start here for a quick overview of the site Help Center Detailed


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|Eddie Guerrero
|October 9, 1967
|El Paso, Texas
|In WWE Games
|First Games
|WWE No Mercy
Eddie Guerrero BioEdit
When Eddie Guerrero flashed that trademark grin, you knew he was already plotting his next move. The big question is what tricks he had in store for his opponent next. Lying, cheating, stealing – it was all fair play in the ring for the unpredictable WWE Hall of Famer.
Beyond his mind games though, Eddie was a performer of remarkable versatility. He could make you hate him, then love him, then wonder where the heck you stand – and that's all in one night. Regardless of whether the context was serious or lighthearted, Guerrero commanded attention from every spectator.
Eddie discovered his unbridled passion for sports-entertainment as a child through his father, longtime wrestling promoter Gory Guerrero. As Gory plied his trade in El Paso, Texas, Eddie was busy scrapping with his nephew Chavo Guerrero Jr. during the show intermissions. Eddie would later move on to wrestle collegiately at New Mexico Highlands University before returning to El Paso to learn how to become a future WWE Superstar.

subset: commoncrawl


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Soft Pretzels
An array of mustards -- ranging from spicy to sweet, smooth to grainy -- adds intrigue to these easy-to-make soft pretzels. Pretzel salt is available from Spice Barn.
- Yield: Makes 12
Source: Martha Stewart Living, October 2008
- 2 1/4 teaspoons dry active yeast (1/4 ounce)
- 1/4 teaspoon coarse salt
- 2 teaspoons sugar
- 1 cup warm water
- 3 cups all-purpose flour, plus more for surface
- 1/8 teaspoon cayenne pepper
- 2 tablespoons unsalted butter, softened
- Vegetable oil, for baking sheets
- 3 tablespoons baking soda
- 1 tablespoon pretzel salt
- Assorted mustards, for serving
Mix yeast, coarse salt, sugar, and warm water in a small bowl, whisking until sugar dissolves. Let stand until foamy, about 5 minutes.
Mix flour and cayenne in a large bowl. Using a pastry cutter or your fingers, cut butter into flour until mixture resembles coarse crumbs.
Slowly pour yeast mixture over flour mixture, stirring to combine. Using your hands, gather dough together. Turn dough out onto a lightly floured surface, and knead until it is no longer sticky, about 5 minutes. Cover with plastic, and let rise for 30 minutes.
Cut dough into 12 pieces, and roll each into an 18-inch rope. Form a U shape with 1 rope, and twist ends together twice. Fold twisted portion backward along center of U shape to form a circle, then gently press ends of rope onto dough to seal. Transfer to an oiled baking sheet, and repeat. Let rise for 20 minutes.
Preheat oven to 475 degrees. Bring a large pot of water to a boil, and add baking soda. Boil pretzels in batches until puffed and slightly shiny, 1 to 2 minutes per side. Transfer to wire racks to drain. Return to baking sheet, sprinkle with pretzel salt, and bake until golden brown and cooked through, about 15 minutes. Pretzels will keep, uncovered, at room temperature for up to 12 hours. Rewarm in a 250-degree oven if desired, and serve with mustards.
Cook's Notes
Reviews Add a comment
JulieMeyer23 SEP, 2012Fun to make. They were a little doughier than I expected, so next time I'll lower the oven temp and bake a little longer. The kids had fun helping and loved the hot bath they got before baking.Reply
Josh Wilson1 AUG, 2012I always love what you make Lucinda. These pretzels are awesome. I didn't let my first batch drain long enough after boiling. Second time was the time. These are delicious.Reply
PeterPaul5 NOV, 2011I made this with whole wheat sourdough bread and things turned out well. My dough was too thick and I used a convection bake instead of regular bake. The pretzels came out good and I still had enough left for my sausage mushroom whole wheat sourdough pizza. Next time I'll roll the dough thinner, turn down the boiling water to a simmer and use a conventional bake.Reply
betsyrisch28 AUG, 2011Bummer- same thing happened to me....the first one I boiled, COMPLETELY fell apart into several pieces. For the next ones, I shaped them into bagel-shape and that worked out ok.Reply
alisaterry28 DEC, 2008The trick to pretzels, I am finding, is to use a somewhat sticky dough and roll the ropes VERY thin. If I don't do them stringy enough, I usually end up with giant poofy knots instead of pretzel shapes. If the dough isn't somewhat sticky, they lose their shape in the water.Reply
coolparkolay11 DEC, 2008I made these pretzels and had a terrible time with the boiling water. They fell into pieces and I am not sure why. I make doughs quite a bit and would love some insight to what may have been the problem, because I do want to make them again.Reply
UrthGuru31 OCT, 2008This recipe was extremely simple. My brother has never made anything from scratch and he and I had a blast making these pretzels, and they were GOOD! To change it up from pretzel salt we also used some roasted minced garlic and some toasted sesame seeds on some.Reply
UrthGuru31 OCT, 2008This recipe was extremely simple. My brother has never made anything from scratch and he and I had a blast making these pretzels, and they were GOOD! To change it up from pretzel salt we also used some roasted minced garlic and some toasted sesame seeds on some.Reply


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Owl much fun would a pool party in February be? We think lots of fun! This owlishly cute birthday invitation features your wording beside a purple owl and surrounded by water-blue and purple colors. Flowers and cute ribbon graphics make this owl pool party birthday invitation design especially girly, although we can completely change any of the colors and graphics to suit twins, a birthday boy, and your personal taste.
To personalize this design further, we can include a small photo beside your wording when you personalize this card design here: Sweet Owl Birthday Invitation.


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How much car insurance costs depends on your age, gender, driving record, the car you drive, even your job.
Remember that one of the best things you can do to save on car insurance has nothing to do.
Our car insurance coverage calculator tells you how much car insurance you need and what drivers like.Discover how Esurance was born online and built to help you save on auto insurance.
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When deciding whether to run an email campaign, one of the most critical things you'll want to know are the rules of email marketing. Who can you send emails to? When and how often? What are you allowed to say? You'll certainly want to brush up on the laws that protect privacy and regulate practice (CAN-SPAM, CASL, GDPR, … Continue reading The Golden Rules of Email Marketing
Category Archives: Web Strategy
Great Products Alone Don't Make Happy Customers
These days, the business process is all about the customer. It's not enough to just have a great product. Customers expect a more interactive approach with each company and purchase. It's all about a collective experience ‒ the search, the decision, the purchase, the support, and the follow through.
Hot Web Design Trends We Don't Love
Important Numbers to Gauge Your Website Success
Analytics data is very important in measuring your website success. Within all those charts, graphs, tables, and numbers lie the answers to all of your marketing questions and conundrums. Did that campaign you paid for drive traffic to your website? How much, and what were the visitors interested in? Did the change you made to your homepage increase your … Continue reading Important Numbers to Gauge Your Website Success
The Importance of an Elevator Pitch
What is an elevator pitch? Sales work often starts from the first impression. In most encounters, you only have 30 seconds to show, a few minutes to impress, and a day to respond. My advice is to prepare an impressive elevator pitch. An elevator pitch is the most minimalist interpretation of the products or services … Continue reading The Importance of an Elevator Pitch
How to Be a Better Client & How It Helps You
Part of the sales process for any business is looking at your current clients and saying, "We need more clients like Company ABC". In some cases, this A-list is determined by how much and how often they buy services. In others, it comes down to the quality of the relationship. We'd never show it outwardly, but it's inevitable that we do end … Continue reading How to Be a Better Client & How It Helps You
Types of Billing to Consider for Marketing Costs
Billing is one of those things that no one wants to think about but is definitely a necessity. Types of billing vary between companies and individuals; the billing structures can depend on the requests of the client or on the discretion of the company. Blayzer has noticed 5 billing options that are popular for marketing and web-development companies … Continue reading Types of Billing to Consider for Marketing Costs
Website Design Process: Old vs. New
Wow. It's already 2016. If you would have asked me when I was a kid what the world would be like today, I'm sure I would been expecting flying cars. Well, we might not have flying cars yet, but the technology of the world has changed dramatically, especially within the online realm. Website design in … Continue reading Website Design Process: Old vs. New
What Makes Blayzer Marketing Better?
In these technology driven times, digital marketing, web development, and lead generation services are growing in popularity. This is a great thing if you are looking for help in this area, but determining who to trust can be difficult. At Blayzer Digital Marketing Group, we are confident that we are the best choice for clients. … Continue reading What Makes Blayzer Marketing Better?
The Rise and Fall of 90's Era Internet Trends
Ahh, the 90's: a time when Seinfeld and Friends were on TV, Jurassic Park and Titanic were dominating the box office, Clinton was in office, and the internet had just started to work its way into the lives of the public. In the beginning, the internet as a whole lacked a strong sense of style … Continue reading The Rise and Fall of 90's Era Internet Trends


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Mortgage Penalties: Just How Much Will it Cost to Break my Mortgage?
Would now a good time to break your mortgage and refinance?
This is a really common concern– when should I break my existing mortgage and refinance for a current best mortgage rate? It's best to initially weigh out the costs.
Breaking your Mortgage
A Canadian mortgage rate agreement is a fully committed contract. There is an out clause, however it comes at a cost.
How Much is my Mortgage Penalty?
Typically the cost is determined based upon either three months worth of interest payments, or the interest rate differential (IRD).
Step 1: Calculate your IRD (Interest Rate Differential)
1) Use the principal balance and multiply it by the difference between your existing mortgage rate, and the new low mortgage rate.
2) Divide that number by 12.
3) Multiply that number by the remaining months in your term to obtain the approximate IRD owed.
Step 2: Calculate 3 Months of Interest
Just simply multiply the amount of interest you would owe on the present mortgage amount. Multiple this by 3.
Step 3: Find out the Penalty you Would Pay
When it comes to a fixed rate you would pay the greater of the IRD, or 3 months of interest. While in a variable rate, you would generally pay 3 months of interest. Contact your mortgage broker or lender to identify your specific required payments.
Step 4: Calculate Your Savings
1) Calculate the interest on your current mortgage rate.
2) Calculate the interest for your new mortgage rate.
3) Calculate your savings.
Step 5: Find out if it is Worth It
Decide if changing is worth it by comparing your expenses to your savings.
Mortgage Term Vs. Amortization
Do you understand the difference between your mortgage term and your amortization period?
A frequent source of confusion for potential homebuyers is the difference between a mortgage term and amortization period. A standard Canadian mortgage rate has a 5-year term with a 25-year amortization period.
Mortgage Term
The mortgage term is the length of time you commit to a low mortgage rate, lender, and associated best mortgage rate terms.
Mortgage Amortization Period
This is the length of time it will take you to repay your whole mortgage. Longer amortization periods lower your month-to-month payments, as you are paying your mortgage off over a greater number of years. But nevertheless, you will pay even more interest over the life of the mortgage.
Maximum Amortization Reduced to 30 years on March 18th, 2011
In January 2011, Minister Flaherty revealed that the maximum amortization duration on all CMHC insured houses would be lowered from 35 to 30 years.
Quite a few home buyers opt for a reduced amortization period leading to greater regular monthly payments if they have the means to do so, understanding that it encourages desirable saving habits and minimizes the overall interest payable.
Short vs. Long Term Amortization Periods
Prepayment privileges set out by your lender will determine whether you can reduce your amortization period, by either enhancing your regular month-to-month payments and/or putting lump sum payments towards the principal. Nevertheless, beyond these privileges, you will typically incur charges for making extra payments. According to the Canadian Association of Mortgage Professionals, 24 % of Canadians benefited from prepayment options in 2009.
Using a Home Equity Loan for Debt Consolidation
Have you considered this popular alternative?
The biggest debt you are going to deal with as a property owner is your mortgage. With the right approvals, you are able to borrow against the equity of your house with a home equity loan.
What is a Home Equity Loan?
This style of loan is somewhat different to a home equity line of credit (HELOC), which is a line of revolving credit with a flexible interest rate. A home equity loan is a one-time lump-sum loan. Lenders are normally worry-free when contributing to your existing low mortgage rate since they are protected by the fact that your loan is secured against your house.
A Tool for Debt Consolidation
The main benefit of a home equity loan is in its debt consolidation abilities.
How do I get a Home Equity Loan?
The best way of obtaining a home equity loan is through a Canadian mortgage rate professional.
Benefits of a Home Equity Loan
Money in your hands to settle outstanding high interest debts.
By paying off outstanding debts you are going to enhance your credit score.
The home equity loan may be spread over the lifespan of your best mortgage rate, typically as much as 25 years.
Tax deductions are readily available.
Downsides of Home Equity Loans
You will be jeopardizing your home if you do not have the means to pay the loan back.
Once you borrow against your house you lose equity or ownership in the home.
Speak to a mortgage broker today to find out if this financial product is the right choice for you.
What are Blended Mortgages?
Blend your mortgage to improve your rate.
Quite a few people are wondering how to lower their current mortgage costs.
Generally, the mortgage penalties you incur to break your mortgage are set up as the greater of three months interest or the value of your Interest Rate Differential. If you're going to break your mortgage, try and do it when your mortgage is sitting in the "sweet spot"– this is when your rate is not high enough to trigger IRD and thus you're only required to pay the three months interest penalty.
Unfortunately, the sweet spot rarely comes at a convenient time. Also, most people will have trouble ever fitting into this scenario if their rate is over 4 %. If this is your situation, speak with your low mortgage rate planner about a blended mortgage. There are two options that most banks will offer:
Blend and Extend or Blend to Term
Under a Blend and Extend option, the bank will give you a brand new term at the current rate but 'blend' in your penalty to your new rate.
The Blend to Term option is the same idea but your term remains as is. For example, you would end up with the same two years left but at a lower rate with the penalty blended in.
If you are in that "sweet spot" a good Canadian mortgage broker will show you calculations on just how much you can save by breaking your best mortgage rate. If you're subject to an IRD, a good planner will go over what blended options are available to you and take into account your time frame and overall goal to help you select the option that's the best fit.


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Sold Out
Size: 10 x 1 Bar
Ingredients: Protein blend (calcium caseinate, whey protein isolate, soy protein isolate), glycerin, maltitol, coating (maltitol, cocoa butter, chocolate liquor, sodium caseinate, milk fat, soy lecithin, natural flavors, salt), cocoa powder, almonds, water, soy crisps (soy protein isolate, rice flour, salt), canola oil, soy lecithin, cocoa powder, chocolate liquor, sea salt, natural flavors.
Nutritional facts: per 1 bar (60 g) : Calories 230, Fat 7 g, Saturated Fat 3.5 g, Trans Fat 0 g, Cholesterol <5 mg, Sodium 180 mg, Carbohydrate 25 g, Fiber 1 g, Sugar 0 g, Sugar Alcohols 14 g, Protein 20 g.
Warning: Contains milk, soy and almonds.
ALLERGEN INFORMATION: Manufactured in a plant that processes eggs, peanuts and tree nuts.
This product contains glycerine which is not a carbohydrate but has 4.32 calories per gram.
Excess consumption may cause a laxative effect.
These natural high protein thinkThin bars are draped in rich delicious chocolate and deliver 20 g of protein. Rate done of the top protein bars in the natural industry, thinkThin is the only naturally sugar-free, high protein bar for an active lifestyle.
Labels change constantly, If you see any errors please let us know so we can fix them thanks!
© 2017 Low Carb Canada.


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신희완님의 아이디어 더 보기
2 by 4 LED profile lighting from About Space
record your voice and then take an image of the sound wave you created and turn it into a framed print - really creative gift idea.
We're talking boards and bricks here. The last time I put boards and bricks together I got a bookcase. Apparently they're like legos.
Japanese origami crane made by cut-out paper
Earth Lights Rock Candles - Set of 3 votive holders in natural shades of gray
lampe origami à faire soi-même en papier plié rose avec un câble jaune
SNOWDROP Origami paper lampshade on Etsy, £59.47
Beautiful paper lanterns add artistic flair to any room. Miss Moss : Humble Abode
Cutting paper
The Quilt Rat: Cutting Paper Snowflakes


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The {Posh} Daisy reached 1,000 Facebook fans recently
and I am thrilled to be taking part in a HUGE giveaway that went live TODAY!!
I have a few pieces from The {Posh} Daisy already, and I love them!
I always get tons of compliments...
so that, PLUS the fact that Cerrisse is such a doll and a good bloggy buddy,
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History of Women's Movements: Part II
Second Wave Feminism, also known as The Women's Liberation Movement, was a wave of feminism from the 1960's to the early 1980's that rallied around women's issues such as gender roles, marital rights, reproductive rights, domestic violence, rape, and divorce laws. Continue reading "History of Women's Movements: Part II"
The Untold Story of Madame X
Agnes May Driscoll, née Meyer (July 24, 1889 – September 16, 1971, Leo/Athena), known by colleagues as Madame X or Miss Aggie. Continue reading "The Untold Story of Madame X"
Preserve the Past to Inspire the Future
When you cracked open a history book as a child in school, you were likely not overwhelmed by the number of women staring back at you. Continue reading "Preserve the Past to Inspire the Future"
History of Women's Movement: Part I
First Wave Feminism occurred in the late 19th to early 20th centuries with the mission of legally changing the rights of women. Continue reading "History of Women's Movement: Part I"
First in Their Field: Rose O'Neill
As a teenager Rose O'Neill started her career as an illustrator in New York City, eventually becoming the first women published cartoonist in the United States. Continue reading "First in Their Field: Rose O'Neill"
Playwright, Cast and Director Speak Out on "Speaking Out"
This month, American History Theater will partner with The Women's Museum of California once again to present "Speaking Out: Why I Stand". Continue reading "Playwright, Cast and Director Speak Out on "Speaking Out""
From Our Archives: Alice Park and the Peace Ship
"The time has come for a few men and women with courage and energy, irrespective of the cost of personal inconvenience, money sacrifice, and criticism, to free the good will of Europe, that it may assert itself for peace and justice with the strong probability that international disarmament can be accomplished." Continue reading "From Our Archives: Alice Park and the Peace Ship"
First in Their Field: Kate Warne
In every field, someone has to be the first. Often, we know their names. Sally Ride. Amelia Earhart. Marie Curie. But often, their names aren't known – either because we don't know who was the first to break a particular barrier, or because the story just hasn't been told often enough. Continue reading "First in Their Field: Kate Warne"
What Women Wore
Learn how women marched forward for their rights by taking a look back at the clothes they wore. Continue reading "What Women Wore"
The History of the Handbag
Bags are not a new invention, as long as humans have had items to carry, we have created bags in which to carry them. As early as 38,000 BCE, hunter-gatherer, humans were using bundles and pouches made from fibers to … Continue reading The History of the Handbag


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Tue Jan 5 2010 Tonight On The X Zone With Rob Mcconnell
martha jette -Glimpses: Correct Stories of the Charming - Martha Jette is a previous essay and munitions store editor, who has detained a harsh stance in the unnatural since she practiced a stance at seven living old. In her 60 living, she has had a specify of unnatural experiences including seeing confidence, extrapolative dreams and a just about momentary interval. In our time, she is retired and has in print a specify of books including Glimpses: Correct Stories of the Charming and Glimpses 2: (it could eject to you!), which contains 90 splendid but fine unnatural tales from reveal the innovation on whatever thing from ghosts and hauntings, to earlier lives, revival, angels, demons, ESP, premonitions, extrapolative dreams and especially. Her upper limit belated concentration in print furthermore co-writer Sunrise Colclasure is called Absolute Scared: The Address Amount On Shadowlike Houses that includes decorous stories from population who in performance in shadowy houses, how to adjudicate and/or charge if your settle is shadowy, who to call out for altruism, because phantom hunters do and especially. http://www.totallyscared.synthasite.com
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jennifer robins -Ouija Boards - Jennifer Robins researches the miraculous and unnatural she writes on the subject of. Having had experiences of her own, she is upper limit inquiring in the unexplainable we in performance furthermore. She lives furthermore her husband and pets in Ohio and loves music, art and the stage the grand piano. Her duty to writing is a add up little job for her and she loves it. www.jenniferrobins.com
Chock 1: 10 pm - 11 pm / 7 pm - 8 pm Placatory
erik stearns -Shaman - Erik Stearns is a man furthermore various talents and careers. He started out in Telephone system act of kindness in 1988. Late reaction his Bachelor's of Science at the Moot of Texas, He began his own fatal consulting eager specializing in logical back and as soon as software fatal - an crack that has to this day yielded various consumers of marvelous and slight companies. In 1995 he began researching the unusual person of Unconcerned Screening and psychic plan. In 1997, as soon as publishing his "Internet demonstration to Networked Existence" gall, he founded the Synergy Scrutinize Cartel - a company that specializes on trade fair civilization how to tap their main shrewd abilities. He is moreover a Shaman who is trained in various background, and serves as a Wonderful Advisor to parts of the public, Screech Mover to two councils, and does Normal American and Shamanic readings for the confusion.
Chock 4: 01 am - 02 am Eastern / 10 pm - 11 pm Placatory
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With Valentines Day Almost Upon Is Lets Talk Goddesses
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A: The histogram chart uses the current colour attributes to subdivide the data. This allows quick visualisation of the relative contributions by different subgroups to the overall sample count. The stacked mode merely displays the coloured bars on top of each other, and thus the totals for each bin would be the same as for a histogram without colour groups (ie only 'default' set in the colour attribute dialog). The layered view plots each group 1 behind the other, with the colours made semi-transparent, and is generally only useful for 2 or 3 groups. The number of bins can be modified using the up and down arrow icons, while individual colour bins or the entire bar can be selected for attributing using the top two icons.
What do the histogram icons mean, and what are the different views?
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Posted by Kenny
Good fella worked hard on my case to my satisfaction would recommend to anyone going through what I had to deal with.
Posted by Derick
I contacted Samuel Terry law firm by accident. I received a call back asking if I needed their assistance. Once I told the representative that I was trying to contact a sibling who was missing and we expected him to be incarcerated, she asked for their information and assured me that she would give me a call back when she got to her computer. An hour or so later she returned my call with a detailed update which included all things from the date of indictment to sentence received and parole eligibility date. I am very appreciative for her assistance. She did not have to assist me in any way. Plus, I failed to mention that all of this took place on a Saturday evening.
If I can receive assistance of this caliber by accident, on a Saturday evening, and free of charge, imagine the level of service they would provide if hired... Words cannot express my gratitude, but thank you
Posted by Kevin
A great defense attorney! Gave me all the info I needed about my case and his staff was very helpful and took care of every question and request I had. I was facing a potential 2nd dwi and with his abilities as a lawyer got it reduced to obstruction of highway. Couldn't be happier and would highly recommend for anyone!
Posted by BenHee
Mr Terry and his assistant Kristie are definitely the best team. Terry is professional and Kristie is very caring about clients. They are opening and work hard. My case was dismissed without damage after 3 month. Appreciate Terry's good work and guidance, and Kristie's patient to listen to me and support. Over there, you are not only a client, and they become the friends to support you.
Posted by Patrick
I was in a bit of a bind facing a possible felony, and civil charges on top of the criminal. I cold called every attorney I could find on avvo and once I talked to him a huge wave of relief was swept over me. And Christine was also a big help. He told me he REALLY wanted to take my case, and I knew at that moment I could trust him. The epitome of bad ass and a true lawyer. You can tell they really care, if you end up in a pickle he's the one to call. Fair, honest, and diligent... this firm has had a significant positive effect on my life. Thank you!!!!
Posted by DUBERNEY ZUNIGA CUERO
I was searching for a layer and I came across Samuel R. Terry I hired him to represent me for my case he was very professional at his work thanks to him and his staff I'm able to have a normal life back thank you so much for helping me out at the same time I want to thank his secretary Kristie for helping me also thank you guys god bless !!!
Posted by Clint Bedell
Very knowledgeable in his field and did a excellent job defending my son. Explained things as we went and advised us the whole way...excellent job. Thank you so much!
Posted by Matt G.
First off I would like to simply say Thank You to Sam and Elan for going out there way on helping me on my situation. I currently moved back home to Las Vegas and got a surprising citation in Dallas from a person who bought my car I sold. With there fast response, quickly they took care of my citation. From there prompt service to excellent communication skills I was very comfortable knowing that they will take care of me. I have never had any service like this the way they did. Very professional and the utmost sense of urgency when it comes to anything. I appreciate and value there business. I definitely recommend them. Thank you again Elan and Sam you two are simply THE BEST!
Posted by Amanda
We hired Mr. Terry for a charge back in June, and from the very start he and his team were very available for all the questions or worries we had. Kristie has been a god send, answering every text, phone call, or email sent. he took care of the case in a timely fashion and delivered what he promised. would definitely hire him again!
Posted by Kimberly Henderson
I contacted Mr Terry late at night in an urgent situation. I was able to retain his firm within a few minutes. Mr Terry and his staff went right to work immediately. They are professional,knowledgeable and put my mind at ease. Words cannot adequately express our gratitude for the work they did. I would highly recommend this lawyer to anyone.


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JustNick Cut File Store
Beachcombing with Friends June 06, 2016 07:00
Life is a Beach August 05, 2015 07:00
Ah summer... It's been super hot here in the pacific northwest so, to channel my inner summer card making was quite easy. I love the sun and summer themed cut files in the shop! I used a couple of faves on the following two cards. This first one uses the waves from the Lake Life cut file
I love the doodle look of the waves and decided to cut them out with white cardstock and use a watercolor effect with my inks.
A close up...
The second card, I grabbed the adorable sun from the Sunny Days cut file.
Once again, I used white cardstock and then used some yellow mist. Since it's summer and I like to make things sparkle, I added some glimmer mist on top, too. The cloud is from the cut file, as well.
SO perfect for cards!
Thanks for swinging by today! Are you in the process of documenting your Summer memories? Make sure to grab some awesome cut files from the shop!
Cut files used: Lake Life and Sunny Days
New designs in the store NOW! July 18, 2015 09:37
Check out the newest designs loaded into the store today!
And don't forget - you can get this cut file for FREE using the code: lakelife at checkout (offer ends midnight CST on July 31, 2015 - no purchase necessary)
Guest Designer Audrey Yeager + a freebie! July 17, 2015 07:00 4 Comments
We welcome Audrey Yeager to the blog today as our Guest Designer for July!
We knew we had to ask Audrey to GDT for us when we saw her great projects at My Scraps and More!
Meet Audrey:
I am a thirty something stay-at-home mom to my kindergartener and pre-schooler. I started scrapbooking around 2005, although I have been "crafty" my entire life, dabbling in different crafts here and there. In 2007 I discovered the online world of scrapbooking, which totally changed the way I scrapbook. Since then, I've been on several teams for kit clubs, manufacturers, and challenge blogs. In January of 2014, I took over ownership of an online retail scrapbook store called My Scraps & More. I have an entire store in my basement, and it allows me the best of both worlds to work part time from home in the store and spend time with my kids, too! (Not to mention I get to scrap with ALL THE THINGS!)
I love to scrap about our everyday moments and try to record all those little things that are said and done. I'm also so glad that we live in a place like upstate NY where we experience every season because I love being able to change up my products and inspiration from one season to the next!
I love how scrapbooking can remove me from anything else I may have on my mind, and just take me to a place where all I think about are colors, patterns, and the memories behind this photo. I also love the social part of it, too, in going to crops and retreats, and the online support and camaraderie!
In addition to scrapbooking, I love to quilt and sew, garden, play volleyball, and watch movies and sci-fi TV series!
We asked Audrey to create a few projects using our cut files and look at what she sent us!
I used the Brushstrokes cut file from the store and cut different "strokes" from different colored papers. I layered the stoke cuts in a vertical line behind my photo. I cut the Simple Scalloped Strips file from white cardstock and added small bits to the top and bottom of my photo as a little border.
We also asked Audrey what type of file she would like to use and she asked for a lake inspired cut file! This is what we sent her:
Check out what Audrey did with this file:
I used the waves from the freebie file and cut them in different sizes. I cut them from apiece of paper that had an ombre pattern to it so that the bigger waves were on darker paper, and the smaller waves were on lighter paper. For the title, I cut the words "fishing" and "lake" from white paper and inked it with navy ink to add a bit more contrast.
Thanks for sharing your great projects with us Audrey!
To follow Audrey and check out more of her work, here is where you can find her:
Blog: http://audreysreflection.blogspot.com/
Facebook: https://www.facebook.com/myscrapsandmore
Instagram: https://instagram.com/audreykit1/ or http://instagram.com/myscrapsandmore/
If you like the Lake Life Freebie - you can download it in the store for a limited time!
Just add the item to your cart and use the code: lakelife at checkout until midnight July 31, 2015 to get this file for free!
After July, the file will still be available in the store, it just won't be free :)


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The Skating Rink
In his 1856 journal, the great American philosopher and naturalist Henry David Thoreau wrote, "I love the winter, with its imprisonment and its cold, for it compels the prisoner to try new fields ...View Article
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Conditions helped by Physical Therapy
Most common complaints prompting people to start Physical Therapy include:
In addition to the above, elite athletic performance enhancement and maintenance care are also offered.


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3-yeast fermentation experiment
- 21 Saturday, 21 January, 12:00
- 1000 Hills Chef School, Durban
3-yeast fermentation experiment
This should be fun. You pick 3 different yeast strains to brew with. No late hops, so we get maximum yeast character out of it. You can choose when to pitch the yeasts and how much of each. This may require a bit of reading to figure out what each yeast will add to a beer, and depending on when you pitch, it will also add different characteristics to the finished beer. We can compare all of our beers and see what each combo of yeasts does. Each brewer entering must just send through a small ...Show the whole text
10 attendees (104 invited)
1000 Hills Chef School
Old Main Road, Bothas Hill
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Transportation Law includes regulations for operators, vehicles and infrastructure; as well as the contract of carriage, regulations and relation between the carrier and passenger in public transport, shipper and cargo owners. Attorneys who practice transportation law represent individuals and businesses in cases involving most aspects of travel and commerce on the ground, in the air, and on the water -- including regulation of private and commercial vehicles, aircraft, and vessels; compliance with transportation safety standards; and oversight of commercial freight shipment activity.
Keokuk is a city in the southeastern part of the U.S. state of Iowa and one of the county seats of Lee County. The other county seat is Fort Madison. The population was 11,427 at the 2000 census. The city is named after Sauk Chief Keokuk, who is buried in Rand Park. It is located in the extreme southeast corner of Iowa where the Des Moines River meets with the Mississippi. It is located at the junction of U.S. Routes 61, 136 and 218. Just across the rivers are the small towns of Hamilton and Warsaw, Illinois, and Alexandria, Missouri. Keokuk along with the city of Fort Madison, are principal cities of the Fort Madison–Keokuk Micropolitan Statistical Area, which includes all of Lee County, Iowa and Clark County, Missouri.
Transportation Law Lawyers In Keokuk Iowa


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In case you want to read my entire blog from start to finish I thought I would pull together this little page of all my posts, ever. I know I personally find this format easier than using Archives.
If I ever get a steady audience, I might even pull out the most popular posts as well.


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Soul Purpose.
The seven members of Soul Purpose play the best of R&B, Motown, Soul, Swing, Blues, Funk, Jazz and New Orleans Funk. Our mission: get the people dancing.
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Providing expert DJ entertainment & top-of-the-line Photo Booths since 1991. Photo Booth from $450 ~ Party DJs from $450 ~ Wedding DJs from $850. We'll Entertain Your Guests The Way YOU Want!
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German American Social Club.
A unique club with its own Bavarian clubhouse and bar nestled on a beautiful piece of property in Putnam Valley. There are two extra special events each year, German Fest in July & Oktoberfest in Sept. Putnam Valley, NY
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Saker Guitar Works.
Saker Guitar Works is an independent fretted instrument specialty shop located at 528 Broadway, Kingston, NY in the Barcone's Music Center building.
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Drummer With Live & Studio Experience .
Available for working situations. Extensive work both in U.S and overseas. Contemp. Jazz, R/B, Neo, Funk, Motown and everything in between. Reliable, Top Gear & Trans. Please Call {845} 707-0567
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unique Hudson Valley Music Lovers per month! Advertise on HVmusic.com. See details on rates and placement.
More about Midnight Slim & LaurieAnne To get your video featured here, add it to your band/artist listing.
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Automatically Generated Texture Coordinates (Direct3D 9)
The system can use the transformed camera-space position or the normal from a vertex as texture coordinates, or it can compute the three element vectors used to address a cubic environment map. Like texture coordinates that you explicitly specify in a vertex, you can use automatically generated texture coordinates as input for texture coordinate transformations.
Automatically generated texture coordinates can significantly reduce the bandwidth required for geometry data by eliminating the need for explicit texture coordinates in the vertex format. In many cases, the texture coordinates that the system generates can be used with transformations to produce special effects. Of course, this is a special-purpose feature, and you will use explicit texture coordinates for many occasions.
Configuring Automatically Generated Texture Coordinates
In C++, the D3DTSS_TEXCOORDINDEX texture-stage state (from the D3DTEXTURESTAGESTATETYPE enumerated type) controls how the system generates texture coordinates.
Normally, this state instructs the system to use a particular set of texture coordinates encoded in the vertex format. When you include the D3DTSS_TCI_CAMERASPACENORMAL, D3DTSS_TCI_CAMERASPACEPOSITION, or D3DTSS_TCI_CAMERASPACEREFLECTIONVECTOR flags in the value that you assign to this state, the system behavior is quite different. If any of these flags are present, the texture stage ignores the texture coordinates within the vertex format in favor of coordinates that the system generates. The meanings for each flag are shown in the following list.
Use the vertex normal, transformed to camera space, as input texture coordinates.
Use the vertex position, transformed to camera space, as input texture coordinates.
Use the reflection vector, transformed to camera space, as input texture coordinates. The reflection vector is computed from the input vertex position and normal vector.
The texture coordinate index flags are mutually exclusive. This example uses:
- The vertex position (in camera-space) as the input texture coordinates for this texture stage
- The wrap mode set in the D3DRENDERSTATE_WRAP1 render state
// Assume d3dDevice is a valid pointer to an IDirect3DDevice9 interface d3dDevice->SetTextureStageState(0, D3DTSS_TEXCOORDINDEX, D3DTSS_TCI_CAMERASPACEPOSITION | 1);
Automatically generated texture coordinates are most useful as input values for a texture coordinate transformation, or to eliminate the need for your application to compute three-element vectors for cubic-environment maps.
Sphere-mapping uses a precomputed (at model time) texture map that contains the entire environment as reflected by a chrome sphere. Direct3D has a texture-coordinate generation feature using render state D3DTSS_TCI_CAMERASPACENORMAL, which takes the normal of the vertex in camera space and puts it through a texture transform to generate texture coordinates.
Related topics
- Texture Coordinate Processing
- Texture Coordinate Transformations (Direct3D 9)
- Cubic Environment Mapping (Direct3D 9)


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Myotonic dystrophy type 1 (DM1), the most common form of muscular dystrophy in adults, is an RNA-mediated disease. Dramatically expanded (CUG) repeats accumulate in nuclei, and sequester RNA-binding proteins such as the splicing regulator MBNL1. We have employed resin-bound dynamic combinatorial chemistry (RBDCC) to identify the first examples of compounds able to inhibit MBNL1 binding to (CUG) repeat RNA. Screening an RBDCL with a theoretical diversity of 11,325 members yielded several molecules with significant selectivity for binding to (CUG) repeat RNA over other sequences. These compounds were also able to inhibit the interaction of GGG-(CUG)109-GGG RNA with MBNL1 in vitro, with Ki values in the low micromolar range.
Myotonic dystrophy type 1 (DM1) is the most common form of muscular dystrophy in adults, affecting 1 in 8000 people.1 DM1 is characterized by multisystemic symptoms, including myotonia, wasting of the muscle, testicular atrophy, cataracts, and cardiac defects. Unlike typical genetic diseases, which follow the traditional central dogma (a mutated gene is transcribed and translated to an altered encoded protein which affects cellular function), DM1 is governed by an RNA mediated mechanism.2 Specifically, DM1 is caused by expansion of CTG repeats located in the 3′ untranslated region of the DMPK (DM protein kinase) gene on chromosome 19q.3 Transcription produces toxic mRNA containing hundreds to thousands of (CUG) repeats, which form long and stable hairpin structures.4 The (CUG) repeat RNA accumulates in nuclear foci, and sequesters RNA binding proteins such as the MBNL (muscleblind) family of splicing regulators.5 (CUG) repeat sequestration of these splicing regulators causes misregulated and aberrant splicing of a variety of gene products including the chloride channel 1, which is a major cause of myotonia in DM (Fig 1).6 Small molecules and peptides capable of binding (CUG) repeat RNA and disrupting its interaction with splicing proteins are highly desirable as potential therapeutic agents to restore normal splicing.
The development and synthesis of small molecules and peptides able to bind RNA with high affinity and selectivity continues to be a major focus of bioorganic chemistry.7,8 Recent advances include the application of aminoglycosides, aminoglycoside derivatives,9 helix threading peptides,10 and other novel compounds to the molecular recognition of RNA sequences involved in several disease states. Unlike DNA,11 there is not yet a canonical set of "rules" which one can follow directly relating nucleotide sequence to the design of a selective binder. Thus, combinatorial strategies are particularly attractive for rapidly identifying and testing new RNA-binding structures,12 although directed design strategies have also found success.13 We recently reported a novel dynamic combinatorial14 screening method, termed Resin-Bound Dynamic Combinatorial Chemistry (RBDCC),15 and its application to the identification of a ligand for an RNA hairpin involved in HIV-1 replication.16 While a recent report from Ludlow and Otto indicates that analysis of large dynamic combinatorial libraries is feasible in some cases,17 the analytical challenges associated with such analyses, much like those associated with the analysis of "static" mixture libraries,18 have kept the size of the vast majority of DCLs well below 100 compounds. RBDCC circumvents traditional DCC constraints on library size by spatial segregation of selected (active) compounds from large diverse libraries. To target RNA binding, a resin-bound dynamic combinatorial library (RBDCL) with a theoretical size of 11,325 members was created from 150 resin-attached, cysteine-containing building blocks and an identical set of solution-phase building blocks. When allowed to undergo disulfide exchange (a robust, mild, and widely used method for DCL equilibration19) under thermodynamic control in the presence of a fluorescently labeled target RNA, components of high-affinity binders are readily identified by physical removal of fluorescent resin beads and subsequent mass spectral analysis. Importantly, RBDCC is an innately competitive assay, since resin-bound library constituents must compete for binding with compounds in solution. We reasoned that screening this RBDCL against a (CUG) repeat RNA (Figure 2) could provide novel lead compounds for DM1. In addition, comparison with the our previous results would constitute an effective test of the ability of the 11,325-member (theoretical) RNA-targeted RBDCL to yield a different ligand when screened against a different sequence from that originally targeted.
In order to ensure only products resulting from disulfide exchange were selected in the screen, we first determined a set of screening, washing, and fluorescence microscopy exposure conditions that prevented misidentification of resin-bound monomer (present as S-t-butyl disulfides) as "hits". RBDCL screening was then performed as previously described in PBS pH 7.2 + 1 mM MgCl2 at 22 °C for 72 hours with 1 μM Cy3-CCG-(CUG)10-CGG RNA (Sequence A).16 Electrophoretic RNA analysis confirmed that no RNA degradation occurred during the experiment. Post-screen fluorescence microscopy identified 4 beads exhibiting significant fluorescence. These four beads, representing components critical to high affinity ligands, were removed via syringe, washed with PBS followed by methanol, and compounds were photolytically cleaved from the resin (50 μl 4:1 MeOH:H2O, 365 nm, 24 hrs). The RBDCC screen is conducted under conditions such that exchange with bead-bound monomers is favored over "nonproductive" exchange in solution: each resin-bound monomer is in a concentration of 2.58 μM (0.86 μM per resin bead, and theoretically three resin beads per monomer), while solution-phase monomers are present at a concentration of 200 nM. As a result, we only observe monomer thiol-S-t-butyl disulfides in the mass spectrum: in this case, we identified library monomers 1-4 as the selected components. The four selected components had a high degree of sequence similarity: (Quin/Pip)-(Asn/Pro)-Cys-Lys. Importantly, these differ from the components obtained from screening this library against the HIV-1 frameshift stimulating RNA stemloop: in that case, 5-5 ((Quin-Cys-Pro-Phe)2) was identified as the highest affinity binder following a secondary screen and independent binding analysis by surface plasmon resonance.
The identities of monomers 1-4 allow for 10 unique possible homo- and heterodisulfides, neglecting terminus differentiation due to bead immobilization; taking this into account raises the number of possible combinations to 16. However, in evaluating the results of an RBDCC screen, we assume that binding will be commutative for a "linear" DCL member (i.e.: A-S-S-B will have the same affinity as B-S-S-A). As the disulfide linking the two monomers A and B is neither directional nor chiral, this will hold absolutely true if the termini of A and B are identical, and will hold in practice if the termini are different but do not contribute significantly to binding to the target. Because the linker and terminal functionality (a propyl amine linker remaining from the synthesis of solution-phase monomers) are different in the library at hand, but are common to all library members, our assumption here is that both of these termini have only a limited, and uniform, role in binding. We recognize that this assumption may be an oversimplification, and hope to examine this issue in greater detail in future efforts. However, it is important to note that secondary screening, coupled with re-synthesis of "hit" compounds and solution-phase affinity testing, control for these assumptions.
In order to confirm mass spectrometry results, and potentially narrow down the number of library hit structures, a secondary screen was conducted in which all 16 possibilities were individually synthesized on bead, and exposed in parallel to 1 μM Cy3-CCG-(CUG)10-CGG RNA. Note that in this case, in contrast to the RBDCC screen, each bead carries only a single compound type, and no equilibration occurs. After 3 hours, beads were washed, and imaged by fluorescence spectroscopy. All beads bearing homo- or heterodimers fluoresced, in contrast to beads bearing thiol-S-t-butyl monomers alone, validating both the RBDCC process and mass spectral analysis (see selected examples in Figure 3). However, in contrast to our previous work, no clear differences in fluorescence intensity were observed among the 16 sets of resin beads, suggesting that all combinations of these four monomers could have similar affinities for RNA sequence A, at least in their resin bead-bound forms. Therefore, the 10 disulfides were individually synthesized in order to permit solution-phase binding analysis. Preparative scale synthesis was carried out on Wang resin with a diaminopropane linker, as in the synthesis of solution-phase monomers. This means that the compounds evaluated in solution bear one more basic group than the resin-bound compounds (4 rather than 3), a systematic change that should affect the binding ability of all compounds similarly.
A filter binding assay system was utilized to provide a rapid initial assessment of binding affinity.20 All binding studies were performed in PBS pH 7.2 + 1 mM MgCl2 at 22 °C. Various concentrations of peptides were incubated with 10 nM FAM-labeled RNA (sequence B: 5′-GGG-(CUG)109-GGG-3′) in a total volume of 50 μl for 20 minutes. A slot blot apparatus was then assembled with a wet nitrocellulose filter, on top of a wet nylon filter, on top of filter paper (Figure 4, left). 40 μl of each binding mixture was then loaded into an individual well of the apparatus and allowed to penetrate the filters for 10 minutes. This assay is predicated on the ability of nitrocellulose to retain peptides, while allowing RNA to pass through. The peptidic disulfides selected from the DCL efficiently bind to the nitrocellulose filter, and in contrast, the FAM-labeled RNA strands penetrate the nitrocellulose filter and are bound by the nylon filter. As such, RNA bound by the peptidic library members remains bound on the nitrocellulose filter, and unbound RNA passes to the nylon filter.
Densitometric analysis of the ratio of labeled RNA on the nitrocellulose to nylon filters allows quantification of binding (Fig 4, left). All binding isotherms were fit to the logistic binding model.21 The repetitive nature of the target sequences most likely result in binding stoichiometries that are more complex than a simple 1:1 interaction; however, as all measurements were performed at [RNA] KD, this does not affect the validity of the reported KD values. As seen in Figure 4, the 10 possible disulfides identified in the screen all bind GGG-(CUG)109-GGG RNA (Sequence B). A suite of experiments run with a 32P-labeled (CUG)56 transcript provided similar results; these are included in the supplementary material. Compounds 3-3, 4-4, 2-4, and 3-4 exhibited the highest affinity (~2 μM) in these experiments, and as such, we chose to focus on these compounds for further study. To test specificity and to confirm the determined binding affinities, filter binding assays and fluorescence titrations were performed with target 5′-FAM-CCG-(CUG)10-CGG-3′ RNA (Seq. A), an RNA incorporating the (CUG) repeat only in the stem (5′-FAM-CCG-(CUG)3-GGCAAC-(CUG)3-CGG-3′, Seq. C), a (CUG) loop RNA (5′-FAM-CGCGCUGCUGCGCG-3′, Seq. D), a (CUG)-(CAG) complementary RNA hairpin (5′-FAM-CCAGCUGGCAACAGCUGG-3′, Seq. E) and the HIV-1 frameshift stimulatory sequence (FSS) RNA hairpin used in our previous study (5′-FAM-GGCCUUCCCACAAGGGAAGGCC-3′, Seq. F). Competition experiments utilizing target RNA (Seq. A) and a 20-fold molar excess of total yeast tRNA were performed to measure non-specific RNA binding.22 Compound 5-5, the HIV FSS RNA ligand, served as a negative control.
As seen in Table 1, compounds 3-3, 4-4, 2-4, and 3-4 all bind (CUG) repeat RNA with similar affinities. Addition of a 40-fold base (20-fold molar) excess of total yeast tRNA results in only an approximately 2-fold loss of affinity to sequence A, as measured by both filter binding assay (FBA) and fluorescence titration (FT). This provides an important demonstration of selectivity for (CUG)n binding.22 Binding to sequences A, B, and C is of the same order of magnitude for all four compounds, as one would expect based on the identically repeating secondary structures predicted for these sequences.4 Curiously, although FBA gave binding constants for sequence D that were similar to those measured for sequences A – C, FT measurements with this sequence did not provide standard saturation profiles, likely due to differences in the two titration methods (vide infra). The importance of the U-U mismatch was assessed by measuring binding to sequence E; FBA indicated a roughly 5-fold decrease in affinity for all compounds, while FT indicated a 10-fold loss for compound 4-4 and >10-fold loss or no saturable binding for other compounds. Sequence F, the HIV-1 frameshift stimulatory sequence employed in our previous RBDCC screen,16 provided another measure of sequence selectivity, and also allowed cross-correlation with the affinity of compound 5-5. This molecule binds sequence F with a KD of 0.35 ± 0.11 μM (as measured by fluorescence titration), while displaying little to no affinity for sequence A (KD > 40 μM). Conversely, compounds 4-4 and 3-4 show very limited affinity for sequence F (KD > 34 μM and KD > 62 μM, respectively).
It is important to note that both filter binding assays and fluorescence titrations have their own idiosyncrasies, and these may contribute to the different levels of sequence selectivity for binding constants obtained using the two methods. Filter binding assays are not true equilibrium methods,23 a complicating factor in their use for the extraction of equilibrium binding constants. Fluorescence titrations, on the other hand, rely on binding-induced changes in a fluorophore tag that may be distant from the actual binding site. Despite these issues, the data clearly show that all four compounds bind, and fluorescence titrations (the preferred method here because it is a true equilibrium technique) show significant selectivity for the target (CUG) repeat sequence.
It has been previously demonstrated that the aggregation of compounds can occasionally confound analysis of library screening results.24 To ensure that aggregation was not a contributing factor in the observed binding, compounds 3-3 and 4-4, 2-4, and 3-4 were examined by dynamic light scattering (DLS). As shown in Table 2, only compound 3-3 showed any evidence of aggregation, albeit at concentrations much higher than those used in our experiments (100 μM and 1 mM in PBS).
Congo Red, a dye known to form aggregates,24 served as a positive control in our experiments. At a concentration of 750 μM in PBS buffer, the dye formed aggregates with a hydrodynamic radius (Hr) of 53 nm. This was clearly evident from the high counts per second (kcps) compared to buffer, and the decay in the autocorrelation function over a 10 to 10,000 μs scale. In contrast, compounds 4-4, 3-4 and 2-4 did not show any aggregation at 1 mM concentration in PBS buffer, as they yielded an autocorrelation function lacking a well-defined decay over time and very low intensities (kcps) close to the intensity value of the PBS buffer alone. However, compound 3-3 showed aggregation at a concentration of 1 mM in PBS buffer. This decreased somewhat with the addition of 0.01% Triton-X 100, as evident from the intensities, but was not completely removed. When tested at a reduced concentration (100 μM), compound 3-3 still appeared to aggregate. It was difficult to detect aggregation at concentrations lower than 100 μM due to low intensities.
We next tested the ability of the selected ligands to inhibit binding of MBNL1 protein to (CUG) repeat RNA, an interaction implicated in type 1 myotonic dystrophy. Peptide-mediated inhibition of (CUG)109-MBNL1 binding was determined using an Enzyme Fragment Complementation (EFC) assay.25 Briefly, this was performed by first immobilizing (CUG)109 RNA to a 96 well plate. Next, the immobilized RNA was incubated with recombinant PL-MBNL1 fusion (where "PL" is the commercial "ProLabel" enzyme donor peptide)26 in the presence of varying concentrations of inhibitor. The "Enzyme Acceptor" (EA-β-galactosidase, or EA-β-gal) complement was then added, and the activity of the resulting plate - (CUG)109 bound (PL-MBNL1)-(EA-β-gal) complex was monitored via a chemiluminsecent substrate. Only the EA-β-Gal bound to the PL-MBNL1 is capable of performing the luminescent reaction, and as such, any luminescence correlates to the amount of MBNL1 bound to the (CUG)109 immobilized on the plate. Wells lacking (CUG)109 RNA served as a background measure of non-specific luminescence, and were subtracted from each experiment to yield a 0% bound value. Wells containing no peptide inhibitor added served as 100% bound. Values of percent bound PL-MBNL1 vs. peptide concentration were plotted, and data were fit to the logistic equation21 to allow extraction of Ki values (Figure 5).
We were pleased to observe that several of the selected compounds inhibit the (CUG)n – MBNL1 interaction with Ki values in the same range as their measured dissociation constants (KD). Importantly, the selected compounds are able to inhibit the (CUG)n – MBNL1 interaction in the presence of ~40-fold base excess of yeast tRNA with only ~2–3 fold loss in Ki. Compounds 1-1 and 5-5 do not show any inhibitory effect, as expected based on their lack of affinity for (CUG)n RNA. A maximum 50% total inhibition was observed, which may be explained by the ability of MBNL1 to bind short sequences of (CUG) RNA, and the possibility that the compounds do not mask all possible MBNL1 binding sites in the (CUG)109 hairpin. It is important to note that small changes in levels of splicing factors, such as MBNL1 sequestration by (CUG) RNA, have large effects on splicing,27 and thus even modest inhibition of the MBNL1 – (CUG) RNA interaction may be therapeutically useful.
In summary, screening an RBDCL with a theoretical size of 11,325 members provided ligands with good affinity and selectivity for (CUG)n repeat RNA, a causative agent of type 1 myotonic dystrophy (DM1). Importantly, the selected ligands are the first examples of compounds able to inhibit the (CUG) repeat RNA – MBNL1 protein interaction. Inhibition of this interaction in vitro for four selected library members was found to occur with low μM Ki values, consistent with measured KD values. These lead compounds provide an excellent platform for ongoing SAR studies aimed at increasing affinity and specificity for (CUG) repeat RNA, as well as efforts to generate compounds suitable for in vivo studies. Finally, these results confirm the utility of the RBDCC method in general, and specifically as a strategy for the rapid generation of sequence-selective RNA binding compounds.
RNA Sequences A, C, D, E, and F were purchased from IDTDNA with RP-HPLC purification. Sequence B was prepared by in vitro transcription as previously described.28 All CUG RNA variants were dissolved in 1X PBS (pH 7.2) + 1 mM MgCl2 and renatured by heating to 80 °C for 2 minutes followed by slow cooling to room temperature (see Supplementary Information for details). Total yeast tRNA was purchased (Fluka 83853, ~20 A260/mg), dissolved in 1X PBS (pH 7.2) + 1 mM MgCl2 and renatured by heating to 80 °C for 2 minutes followed by slow cooling to room temperature.
Synthesis of the 150 library building blocks on-bead has been described previously.16 Following library synthesis, library screening proceeded as follows: first, control experiments were conducted to ensure that monomeric resin-bound building blocks do not bind the target RNA to any appreciable extent. In triplicate, in 1.5 mL solid phase reaction vessels (BioRad Econo-Pac chromatography columns), 450 beads (150 of each size, 3 beads/compound, 387 μM total) were incubated with Cy3-labeled (CUG)10 RNA sequence A (1 μM) in 1X PBS + 1 mM MgCl2, pH 7.2. This solution was agitated for 3 hours on a LabQuake™ rotator. The solution was then drained from the resin by vacuum, and the beads in each vessel were washed three times with buffer (1 mL, 1 min. each). After washing, the resin was suspended in 2 mL buffer and transferred to a Petri dish. Imaging the resin under a fluorescence microscope equipped with a Cy3 filter showed that no resin beads exhibited a fluorescence signal, suggesting that none of the library building block monomers (thiol S-t-butyl protected) bound the target RNA.
Next, the RBDCL was screened against the target RNA Sequence A. A heterogeneous mixture of solution-phase library constituents (30 μM based on average molecular weight) was incubated with resin-bound library constituents (450 beads; 150 of each size, 3 beads per compound, 387 μM total concentration of resin-bound monomer based on average molecular weight), and Cy3-labeled (CUG)10 RNA Sequence A (1 μM) in 1X PBS + 1 mM MgCl2, pH 7.2. Libraries were equilibrated in quadruplet for 72 hours, a period of time shown by HPLC to be sufficient for equilibrium to be reached. The resin was then drained, washed 4 times with PBS + 1 mM MgCl2 for 90 seconds each, plated with 2 mL buffer in a Petri dish, and analyzed by fluorescence microscopy.
After screening, four beads exhibited significant fluorescence, and were removed via syringe and washed 5x with 1 mL 1X PBS, pH 7.2, for 5 minutes, then 5x with 1 mL methanol, for 5 minutes. The size of each selected bead was determined to be medium, thus identifying each of the bead-bound compounds had cysteine at the second amino acid position (Figure 2). Then, each bead was subjected to photolytic cleavage (365 nm) for 24 hours in Eppendorf tubes containing 100 μL acetonitrile:methanol (4:1) to allow identification of binding library members. The resulting solution, which contained the putative RNA binding library members cleaved from the resin was analyzed by mass spectrometry.
Selected DCL monomers 1, 2, 3, and 4 were synthesized on solid phase using standard FMOC main chain and Boc/Trt side chain protecting chemistry. Briefly, each monomer was synthesized on Wang resin (100–200 mesh size, 1 mmol/g loading, 500 mg, 0.5 mmole). First, the resin was activated through the addition of 1-1′-carbonyl-di-imidizole (1620 mg, 5 mmol, 10 eq) in 12 mL of DMF. This suspension was rotated on a LabQuake™ rotator for 12 hours. The vessel was then evacuated and washed three times with 15 mL DCM. Propane diamine (421 μL, 5 mmol, 10 eq) was added in 12 ml of DMF and rotated for an additional 12 hours. The resin was then washed 6X with DCM and 6X with DMF. FMOC-Lys(Boc)-OH (702.5 mg, 1.5 mmol, 3 eq), HBTU (570 mg, 1.5 mmol, 3 eq), and DIPEA (424 μL, 2.5 mmol, 5eq) in 10 mL DMF, was added to each batch of resin, rotated for 1 hour, and the resin was washed. Then FMOC was removed (20% piperidine/DMF, 30 mins.) and resin was washed. FMOC-Cys(Trt)-OH (878 mg, 1.5 mmol, 3 eq), HBTU (570 mg, 1.5 mmol, 3 eq), and DIPEA (424 μL, 2.5 mmol, 5eq) in 10 ml DMF was added to each batch of resin, rotated for 1 hour, and the resin was washed. FMOC was removed with (20% piperidine/DMF, 30 mins.) and resin was washed. Then either FMOC-Asn(Trt)-OH (895 mg, 1.5 mmol, 3 eq) for compounds 1 and 2, or FMOC-Pro-OH (506 mg, 1.5 mmol, 3 eq) for compounds 3 and 4, HBTU (570 mg, 1.5 mmol, 3 eq), and DIPEA (424 μL, 2.5 mmol, 5 eq) in 10 mL DMF was added, rotated for 1 hour, and the resin was washed. FMOC was removed with (20% piperidine/DMF, 30 mins.) and resin was washed. Finally, piperonylic acid (250 mg, 1.5 mmol, 3 eq) for compounds 1 and 3 or 3-carboxy-2-ethyl-3-quinolinium chloride (353 mg, 1.5 mmol, 3 eq) for compounds 2 and 4, HBTU (570 mg, 1.5 mmol, 3 eq), and DIPEA (424 μL, 2.5 mmol, 5 eq) in 10 mL DMF was added and rotated for 1 hour and the resin was washed. Final products were cleaved from the resin and Boc/Trt groups were removed by treatment with 10 mL of a 1% triethyl silane/50% TFA solution in DCM for 2 hours. Products were purified by precipitation in chilled ether (−20 °C). Solids were concentrated by centrifugation (2500 rpm, 10 min), the solution was removed, and fresh ether was added. The solution was mixed by vortex and solids were again concentrated by centrifugation. This series was repeated five times. After the last washing step the solids were dried by lyophilization, resulting in off white powders.
DCL disulfides 1-1, 1-2, 1-3, 1-4, 2-2, 2-3, 2-4, 3-3, 3-4, and 4-4 were prepared by mixing equimolar amounts of 1, 2, 3, or 4 with 1, 2, 3, or 4 in water, and allowing them to undergo oxidative disulfide formation for a period of 7 days. Disulfide formation was monitored by HPLC. When disulfide formation had reached completion, the resulting desired disulfides were separated and purified by preparative reverse-phase HPLC using a 0% to 40% acetonitrile:water (0.1% TFA) gradient.
Fluorescence titrations were performed using a Varian Cary Eclipse spectrophotometer. 2 μl of 50 or 500 μM compound were titrated into 400 μl of FAM labeled RNA sequences, and allowed to equilibrate for at least 10 minutes, or until no change in fluorescence spectra was observed. Changes in fluorescence emission at 518 nm (excitation at 490 nm) were measured. Raw data were corrected for dilution dependant changes, and Em518 was plotted against peptide concentration and fit to the one site binding equation y = (bmax *x)/(KD+x).
Dynamic light scattering (DLS) data were collected on a DynaPro 801 Molecular Sizing Instrument (Protein Solutions Inc). All measurements were recorded at 22 °C. The compounds were dissolved in 1X PBS buffer (pH 7.2). Pre-filtered samples were injected into a 12 μL cell and illuminated by a 25 mW laser at 750 nm. The detector angle was 90°. The hydrodynamic radius (Hr) was calculated using the dynamic software package version 4.0. Ten repeat measurements were performed on each sample and the data was filtered based on sum of squares (SOS) and baseline error calculations. Each reported intensity value is an average of three or more measurements.
A DiscoveRx PathHunter ProLabel Enzyme Fragment Complementation assay was employed to test the ability of selected ligands to disrupt CUG repeat RNA – MBNL1 protein interaction in vitro.
To coat 96 well plates with (CUG)109, 50 μl of 0.5 μM 5′ biotinylated DNA (5′-TTTTAATTTTAGGATCCCCCCAG-3′) in 50 mM Na2HPO4 (pH 8.5) + 1 mM EDTA was immobilized on white streptavidin-coated 96 well plates (Pierce 15502) for 18 hours, and the plate was aspirated and washed. To this oligo, 50 μl of a 0.1 μM solution of a (CUG)109 transcript 5′-GGG(CUG)108CUGGGGGGAUCCUAAAAUUAAAA)-3′ (underlined sequence is complementary to biotinylated DNA) was hybridized in Buffer A (50 mM tris, pH 8.0, 50 mM NaCl, 50 mM KCl, 1 mM MgCl2) for 2 hours, and the plate was aspirated and washed 1x with Buffer A. This process immobilizes ~ 0.5 pmole of (CUG)109 RNA per well.
Various concentrations of compounds were then added with or without 40-fold base excess of yeast tRNA in a total volume of 60 μl in Buffer A and incubated for 30 minutes. For competition experiments, 100 pmole of tRNA was added to the binding mixture (~40 fold base excess, or ~20 fold molar excess). Then, 10 μl of 1 μM (Buffer A + 0.05% tween) recombinant MBNL1 protein containing an N-terminal β-Gal (PL) (PL = ProLabel) Enzyme Donor sequence (PL-MBNL1 fusion protein) was added, incubated for 1 hour, and the plate was aspirated and washed 1x with Buffer A + 0.05% tween. At this step, any PL-MBNL1 protein bound to the (CUG)109 RNA remains bound to the 96 well plate.
The Enzyme Fragment Complementation (EFC) assay utilizes the DiscoveRx PathHunter ProLabel Detection kit. 50 μl of 50x diluted solution of the supplied β-Gal Enzyme Acceptor in PBS pH 7.2 is added and incubated for 1 hour. Then, 10 μl of 100x diluted solution of the supplied β-Gal substrate (Galacton + Emerald luminescence enhancer, DiscoveRx) is added and incubated for 1 hour.
Only the β-Gal Enzyme Acceptor bound to the PL-MBNL1 Enzyme Donor is capable of producing the luminescent product, and as such, any luminescence correlates to the amount of (CUG)109 bound MBNL1. Luminescence of each well was then read. Wells without added (CUG)109 RNA served as a background measure of non-specific luminescence, and were subtracted from each experiment to yield a 0% bound value. Wells containing no peptide inhibitor added served as 100% bound. Percent bound PL-MBNL1 vs. peptide concentration was plotted, and data were fit to a logistic binding model to allow extraction of Ki values.
Full spectral characterization of compounds, filter binding assays, fluorescence titrations, inhibition studies and experimental detail (35 pp).
Financial support from Paul D. Wellstone Muscular Dystrophy Cooperative Research Center 08U54NS048843-05 and NS58345 is gratefully acknowledged. PCG and BRM were supported by NIH T32AR007472. We thank Prof. Joseph E. Wedekind for the use of his dynamic light scattering instrument, and Jolanta Krucinska for assistance with conducting DLS measurements. We also thank Dr. Alice Bergmann (University of Buffalo) for acquiring mass spectrometry data (NSF award CHE0091977).
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Senators Debate Alternative Energy Tax Breaks
Date: 28-Jan-09
Country: US
Author: Tom Doggett
WASHINGTON - The Senate Finance Committee on Tuesday began debating some $31 billion in tax credits and financial incentives to boost alternative energy supplies and promote energy-savings steps as part of the Obama administration's much bigger U.S. economic recovery plan.
The tax breaks being considered would, in part, help wind power and solar energy companies that are having a difficult time getting financing because of tight credit conditions. The incentives also come at time that sharply lower petroleum prices have made alternative energy projects less cost competitive.
The Senate tax package includes most of the $20 billion in energy tax breaks cleared last week by the House Ways and Means Committee, but the Senate measure adds more incentives to help alternative energy companies.
Like the House's legislation, the Senate measure would help the wind and solar energy industries, promote energy-efficiency improvements to existing homes and help service stations recoup their costs for installing alternative energy pumps.
"These incentives would create green jobs producing the next generation of renewable energy sources -- wind, solar, geothermal," said Sen. Max Baucus, who chairs the finance panel. "And they would help to combat climate change by reducing our use of carbon-emitting fuels."
Both the Senate and House bills would extend by three years, to the end of 2012, the date that wind facilities would have to be in place to be eligible for the federal renewable energy production tax credit.
Other qualifying facilities that generate electricity from renewable energy sources, such as biomass, geothermal, small irrigation, hydropower, landfill gas and ocean currents, would also have an extra three years through the end of 2013 to be in service to get the same production tax credit.
With many renewable energy projects having a hard time getting financing, both the Senate and House plans would allow such facilities in place in 2009 and 2010 to temporarily claim a 30 percent investment tax credit instead of the production tax credit that is normally paid out over 10 years.
The Senate measure would do more than the House to help alternative energy companies by allowing them to carryback their business credits from 2008 and 2009 for five years instead of just one year as present law allows to offset their next income taxes. The tax break would cost $11 billion over 10 years.
Other energy tax breaks and financial incentives in the Senate and House bills would:
* Increase the tax credit for service stations that install pumps that dispense alternative energy fuels like hydrogen, natural gas and gasoline made from 85 percent ethanol.
* Give homeowners a tax credit equal to 30 percent (capped at $1,500) of the amount they paid for energy-efficient furnaces, hot water boilers and other energy savings improvements.
* Authorize $1.6 billion in new clean renewable energy bonds to finance facilities that generate electricity from wind, biomass, geothermal, small irrigation, hydropower, landfill gas, ocean currents and trash burning facilities;
* Allow states to issue $2.4 billion in conservation tax credit bonds to finance loans and grants to individual homeowners to retrofit existing housing.
* Provide individuals with a 30 percent uncapped tax credit for buying solar water heating property, small wind energy property and geothermal heat pumps;
The Democratic-controlled Congress hopes to work out differences between the Senate and House bills and have a final package on Obama's desk for his signature into law by the Presidents Day holiday on February 16.
(Editing by Marguerita Choy)


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2013-11-06 19:13:38| 分类: Fusion Cooking | 标签: |举报 |字号大中小 订阅
Note: Not all kind of fish can be baked, especially if it has lots of oil usually around the bone leading to its dorsal (top) and anal (bottom) fins. Fish with lots of oils should be oil-fried at low heat.
1) Clean the fish. The use of a cutter (shown below) makes trimming the fins neat and easy.
2)Wipe off the excess water from the surface of the fish with a piece of dry cloth.
3)Place six pieces of wooden chop sticks on the floor of the frying pan. The aim is to create a gap in between the fish and the frying pan and avoid the fish being in direct with the hot frying pan, hence, eliminating any chance of charring the fish. Both sides of the fish can be baked evenly.
4)Place the fish on top of the chop sticks. Add a teaspoon of Huadiaojiu (two teaspoon if the fish is large or of the smelly type).The gap allows the Huadiaojiu to reach the bottom of the fish easily.
5)Put the lid on. Switch on to low heat.
6)Below is a sample of a baked fish. Note: The skin of this type of fish is very thin and delicate. Yet, other than the tail portion where the fish is in direct contact with the frying pan, the skin on the rest of the fish is fully intact.
7)Add some thinly sliced red chili. It looks much more pleasing.
Remark 1:The fish oil that dripped onto the base of the frying pan must be thrown away. The fish oil of most type of fishes is awful in taste. How many of you can take cod liver oil?
Remark 2: You must be able to pick fishes that do not have much oil at around its dorsal of anal fins.
Remark 3: As for the sauce, I leave it to you because it is your taste buds that matter.
Please read complete procedure at "How to bake fish - a new found method" at url http://ericwoonct.blog.163.com/blog/static/181911362201393043020668/
Dear Readers,
1) Why do I begin to learn to cook at 53 years old? I cannot stand the current pool of chef any longer because they are all "MSG" (full name, ajinomoto) chefs. Every time without fail, an hour after eating any dish cooked with MSG, I feel very sleepy and drowsy.
2) As long as a mother can cook good dishes, her children will never forget her kindness (mother's love).
3) Please forward it to all the mother or anyone who aspire to cook one day (why not, now).
4) Good delicious food enhances the intimate relationship between a couple (mother and child) more than anything else.
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We slept in an amazing hostel! We totally recommend that to anyone. It was so nice all over: Nice showers, nice staff, good beds, good house, good location and an amazing breakfast for the even more amazing price of 5 €. It is called Louise 20. After we went to the recommended burger joint and another restaurant we went to bed, cause our Friday promised to be pretty exciting: 170 km with about 2000 m worth of heights all on single speed/fixed. Check out the route!
It was so beautiful, both physically and the view.
Yeah and we met a Lama along the way, who figures?
It was really hard climbing all that especially with the headwinds still strong. But for me as the fixed gear rider the descents were also very challenging. I was screaming like a school girl - so much fun!
Rolling into Prague is a ball! It goes downhill for minutes, the street turning and turning so that you are asking yourself why it you dont cross it again. Super fun. There were some confusion with the hotel we booked, which was hard for us to take because we were kinda tired, but it turned out that the substitute we got was way more fancy and held a neat opportunity for us... later more. We went to the Chinese restaurant across the street and ate a bunch and of cause had some beer. First Prague highlight was the Chinese grandma sitting across the room burping constantly, hilarious!
The next day was filled with sightseeing and just walking through Prague:
Beautiful weather! We stopped in a park with an outdoor restaurant, ate and had beer. A lot of it actually - it is Prague after all. As we finally got back to the hotel we took the bikes to the room, for the next days preparations and then it was that I came up with the most intriguing idea: RIDING THE HOTEL CORRIDORS! The corridor was a full square, so you would not have to turn around, only corner sharply. ROCK'N'ROLL. Nough said.
Florian compiled this compelling video.
PRAGUE MAY 2012 from Florian on Vimeo.
The way back to Dresden was like flying. Perfect wind and perfect weather. Also I won the sprint to Dresden without even realizing it...
Perfect weekend!!
All pictures taken by Florian
Berlin - Dresden route
Dresden - Prague
Prague - Dresden, flat


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When my son was small and we were just beginning our homeschool journey, my good friend, Tanya, loaned me her Sonlight curriculum for grade 1. Although I chose not to use it, I did read most of the books to my little boy. I have wonderful memories of sitting under the big maple tree in our backyard reading one of our favorites, "Little Pear," by Eleanor Frances Lattimore. In this enchanting book, the main character's favorite treat to buy is a tang hulur. My child liked this book so much that we borrowed the others in the series from the library. Our favorite part that has stuck with us all these years (he is 12 now, but still likes to be read to!) is the fascinating idea of a tang hulur. We have our own idea of what they look like, and when we see a resemblance of our conception, whether it be in a store or picture, we always exclaim, "Look! It's a tang hulur!"
In this blog post, I share with you the day we made tang hulurs, better known as rock candy. This is a fun activity to go along with a science lesson or unit on rocks. We actually made the rock candy with our small Pathfinder group as part of the Rocks and Minerals honor. We had attended a gem and fossil show the week before, a first for all of us. A day or two later, I received an email to sign up for a free online science class to learn about rocks, including experiments to do at home. Thinking this was perfect timing, I bought the necessary supplies for the experiments and showed the video to the Pathfinders. To be honest, the video wasn't a big hit, but everybody loves an edible experiment so that saved the day!
Here is what we did to create our version of tang hulurs. This makes a big batch, so you might want to reduce it. We added eight cups of sugar, which was a small four-pound bag, to three cups of water gradually, and then heated it on the stove. Do not let it boil. We did not use a candy thermometer, but you can. The mixture should change into a cloudy yellowish color with all the sugar dissolved, and should be hot to the touch. Let it cool enough to pour into a glass container. We used mason jars. You can add flavorings and/or colors at this point.
Then position a skewer in the middle, holding it in place with a clothespin laid across the top.
The skewer should be moistened and rolled in sugar to give the crystals something to adhere to.
Now you just wait for the crystals to form. That can take hours or even days; we just kept checking ours.
Break up the edges and pour out the excess liquid after it has crystallized, and set the jar in hot water to remove your creation. It's not the healthiest treat, but fun to make to demonstrate crystals when studying rocks. Enjoy your tang hulur while reading a good book, like "Little Pear," or even a book about rocks.
Oh, and by the way, here's what tang hulurs really look like! Much more tasty to me!


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Ultimately, I went into this play not sure what to expect. I came out delighted and wanting to see more. With so much negativity in the world, we need more of Bank Robbery's and Mischief's outrageous, bright and inventive brand of theatre. We need more comedy that is both simple and inventive, and this is exactly that. I'd go again in a heartbeat.
Category: Theatre
The End of Longing @ Playhouse Theatre, 29/2/16
A few months back, my flatmate told me about how Chandler from Friends had written a play that was coming to the West End. And that he was also going to be in it. She was very excited about it. We booked our tickets at the end of January, and last week we actually saw Matthew … Continue reading The End of Longing @ Playhouse Theatre, 29/2/16


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Powerpoint repairs in Strathfield
What a mess to imagine when one of the powerpoints becomes faulty in the house! You are forced to live without fans, lights, computer and hot water – worst nightmare is mobile phone with empty battery; even if it is for few minutes, it needs immediate ending and here comes the role of an Electrician to the Rescue professional. Our GPS tracked experts can reach you within minutes and bail you out of any such troublesome situation in a blink.
Powerpoint repair in Strathfield is our strength
If you need powerpoint installation in Strathfield newly bought home, you can simply rely on us for the perfect work. We keep our professionals updated with latest trends and requisite skills and all our solutions are safety standards approved. Our professionals at Electrician to the Rescue are licensed, trained and fully equipped with tools for providing you on-spot services.
Powerpoint repairs need to be done by licensed professionals only. You should not even think of doing it on own else you may fall in trouble.
Why to choose us for powerpoint repairs
Choose us for powerpoint repairs and feel the difference! You will find with us:
- 24/7 service: We are available round the clock to attend your emergency calls and for fixing up appointments.
- Fully trained experts: We have developed our team by training them in all sorts of electric repair works including powerpoint maintenance. We send them on field only after complete training.
- Guaranteed services: Our job is to solve your electric problems forever. We take full guarantee for lifetime for all the electric repairs done.
- On –spot solutions: You will find us leaving your premises only after correcting the fault to satisfaction. We install powerpoint in Strathfield with unmatched perfection in single visit bothering you the least.
We are the most trusted name in Inner West for powerpoint repairs. You can book your installations at firstname.lastname@example.com and call us for emergency service at 1800 893 218. You are free to drop in to our office for one-to-one interaction also. Our address is 86 May St, St Peters NSW 2044, Australia.