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Cold Creepy Feeling - Paranormal Exorcism
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A young couple, Lisa Younger and Jared Vandenberg, escape the Los Angeles rat race for the quiet life in Joshua Tree, California but paranormal visions and voices quickly turn the house of their dreams into a house of nightmares. They enlist the help of a spiritualist, Alex Damiano, to help exorcise the premises but this only awakens and angers the demonic presence that lurks within.
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Dog-friendly Battle, 1 Beds & Pub Within 1 Mile
One of two stylishly converted, single-storey holiday barns enjoying a delightful rural setting with glorious views and footpaths from the door across the countryside. Within walking distance of the picturesque village of Sedlescombe with a village green, a historic friendly pub and good local shop.
This converted wing of the owner's home makes a perfect holiday retreat and offers access to footpaths over the Sussex countryside. Sedlescombe with its village green, pub and shop is within walking distance. Attractions include Sedlescombe Organic Vineyard, Battle, the Jerwood Gallery at Hastings and traditional…
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Profile | Photos | Discography | CalendarBorn in Chicago, composer and violinist Leroy Jenkins was one of the most important musicians to emerge from the AACM (Association for the Advancement of Creative Musicians), the legendary collective of which he was a member until his...
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$ 242.80 $ 350.00
With the lighter traveler in mind weve taken our elegant top grain Nappa leather Euro Traveler bag and slimmed it down to include two zippered interior pockets. A fusion of fashion and function the chic timeless design provides ample room for all your carry-on travel essentials with 2 large zippered interior pockets for convenient access.
Dimensions: 14 x 10 x 7
Material: Top Grain Nappa Leather
Colors: Black Tan
Weight: 2 lbs.
Country of Origin: China
$ 229.74 $ 263.80
Burk's Bay Premium 20" Leather Rolling Duffel Carry On A premium leather duffel bag that rolls and which is a carry on. Heavy duty bag that is great for travel....
$ 297.58 $ 319.80
Burk's Bay Premium 22" Leather Rolling Traveler Carry On A premium leather spinner carry on that the travel warrior will want. A top grain cowhide rolling traveler with telescoping handle....
$ 123.00 $ 163.99
The duffel is a legal carry-on size with six antique brass bottom feet. The bag has protective polydrill lining and a matching luggage tag for identification. The shoulder strap has...


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Blueberry Spinach Salad
Recipe by Charlotte J
Spinach, blueberries, and blue cheese in a raspberry vinegar dressing may sound like a strange combination but give it a try.
Top Review by Toni1
I made this salad but used Ken's lite raspberry vinagrette for a short cut. Also used gorgonzola instead of blue cheese because I had it on hand. My husband and I both loved this salad. The toasted pecans really make the salad but we love blueberries and this is a great way to use them during season. Only reason I give it 4 stars instead of 5 is because I didn't make the dressing so I don't know how that changes the taste.
See Discussion:3 Reviews
Ingredients Nutrition
- 1⁄2 cup vegetable oil
- 1⁄4 cup raspberry vinegar
- 2 teaspoons Dijon mustard
- 1 teaspoon sugar
- 1⁄2 teaspoon salt
- 1 (10 ounce) package fresh spinach, torn
- 4 ounces blue cheese, crumbled
- 1 cup fresh blueberries
- 1⁄2 cup chopped pecans, toasted
Directions See How It's Made
- In a jar with tight-fitting lid, combine first five ingredients and shake well.
- In a large salad bowl, toss the spinach, blue cheese, blueberries and pecans.
- Add dressing and toss gently.
- Serve immediately.


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The Complete Collection of Leesa Bed Reviews – the Best Bed In A Box?
In their quest for the perfect sleep at night, most people turn to the biggest, most established brand names first. It's only natural, I suppose. But there are other options you may want to consider. I've looked at alot of Lessa Bed reviews to provide you with an in-depth look at one of the most well-known and popular bed in a box.


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Call Centers Terms and Technology
Are you new to call centers or thinking of carving a career path for yourself in the industry? Before you head out the door for your first interview, get acquainted with some of the terms and technologies you will be faced with. Call centers employ a wide variety of different technologies. This article covers the most common terms and technologies agents are expected to understand and use.
ACD (Automatic call distribution): Part of the CTI that distributes in-coming calls to a group of agents. They are used in companies that take high volumes of calls, where callers require quick service from non-specific agents. More sophisticated systems may route calls to more skilled agents, depending on the reason for the call.
ACW (After call work): Amount of time an agent spends after the call processing customer requests.
AHT (Average handling time): The average time a call takes, including greeting, conversation, wrap-up, and time the caller spent on hold.
ANI (Automatic number identification): Similar to caller ID, a service which provides the receiver of a call with the number of the calling phone. Used in call centers to forward calls to appropriate agents or geographic areas. Also used by 911 dispatchers.
ASR (Automatic speech recognition): Technology used to provide information and forward calls, which allows callers to speak entries rather than punch numbers on a keypad.
ATT (Average talk time): Average amount of time an agent spends in conversation with a caller.
Call Center: A centralized office used to receive and transmit a large volume of requests by the telephone, usually with some amount of computer automation.
Chatterbot: A program that simulates human conversation. An intelligent virtual agent is an example of a chatterbot program that serves as an online customer service representative.
Collaborative Browsing (co-browsing): A technique used by agents to interact with customers using the customer's web browser to lead them through a situation. May use email, fax, regular and/ or internet telephone as part of the interaction.
Contact Center: A part of an enterprise's overall CRM which manages customer contact, including letters, faxes, emails, newsletters, mail catalogues, Web site inquiries, and other gathered information.
CPH/ IPH (Calls/ inquiries per hour): Average number calls or inquiries an agent handles per hour.
CRM (Customer relationship management): A corporate level approach for managing an organization's relationship with its clients. Generally, three components (operational, analytical, and collaborative) of a company's program must be in place in order to effectively acquire, provide services for, and retain customers. Also called Sales force automation (SFA).
CTI (Computer telephony integration): The technology that coordinates between telephone and computer systems.
Customer Service Chat: An internet service which allows a customer to communicate with an agent using an IM (instant messaging) application.
DID (Direct inward dialing): A service used by inbound call centers to allow multiple calls to be taken at once. In DID a block of telephone numbers is rented by a company without requiring a physical line for each number. Each agent or workstation has an individual number. When all agents are busy, additional inbound calls get busy signals or the agent's voice mailbox. This service saves the cost of a switchboard operator and makes calls go through faster.
DNIS (Dialed number identification service): A service used by 800 and 900 lines that tell which number was called. It is useful for directing calls when companies deal with multiple numbers at the same location.
DTMF (Dual tone multi-frequency): Also known as "touchtone" phone (formerly a registered trademark of AT&T), the signals that are generated when a caller presses the touch keys of an ordinary telephone. Each key generates two tones, and cannot be imitated by voice.
Fax: Material (images or text) which is scanned and transmitted over a telephone line and received using a printer or other output device.
FCR (First call resolution): A call which completely resolves the customer's issue. (A call is considered FCR if the caller does not call back with concerns in a set amount of time, usually 3 months.)
Idle time: Percentage of time agents spend not ready to take calls.
IP telephony (Internet protocol telephony): A general term for the technologies that use the internet protocol's packet-switched connections to exchange voice, fax, and other forms of information.
ITS (Issue tracking system): A program that follows the progress of every problem a system user identifies until the issue is solved.
IVR (Interactive voice response): A computerized system at the front-end of calling centers which uses prerecorded prompts to identify caller needs, extract necessary information, and direct calls to the appropriate agent. Whereas, callers select options from voice menus using the telephone keypad, the newest technology, or Guided Speech IVR, integrates live agents into the system. In this hybrid model, agents assist in four or more calls at a time by listening and guiding callers through the system. This allows callers to respond to open-ended questions and receive a higher quality of service. Companies see higher rates of call completion and customer satisfaction using the new technology.
LEC (Local exchange carrier): The public telephone company which provides local service in an area.
Media gateway: A device that converts data from one format to another.
Outsourcing: The practice of delegating non-core operations to an external entity.
PBX (Private branch exchange): A cost-efficient system that uses multiple phone lines (called "trunk lines") and a computer to manage the switching of calls within a company. As the PBX is owned by the company rather than the LEC, it saves the cost of requiring a line for each user to the telephone company's central office.
Personalization: The process of tailoring internet pages to a customer's preferences.
Predictive dialer: A computerized system that dials telephone numbers, filtering out unanswered calls, busy signals, disconnected lines, and other unproductive calls. Using an algorithm to predict agent availability, the system saves the time an agent would spend in unproductive dialing. Smart predictive dialers use a prerecorded introductory message before connecting customers to an agent, further increasing productivity by turning over calls only to interested customers.
Predictive technology: Tools that analyze patterns and use discoveries to forecast likely future behavior.
QED (Quality and efficiency driven): Philosophy maintained by call centers that company strategies should be balanced between aims for quality and efficiency.
Queue: A line of people or calls waiting to be handled, usually in sequential order. Real Time: Level of computer responsiveness considered sufficient to the task required.
SL% (Service level percent): Percentage of calls answered within the determined time frame.
Speech/ Voice Recognition: Ability of a program to recognize and carry-out voice commands. More sophisticated software has the ability to accept natural speech, or the speech used in general conversation.
Telemarketing: A registered trademark of Nadji Tehrani, referring to the form of direct marketing using the telephone to sell products and/ or services.
TCA (Total calls abandoned): the number of calls abandoned by callers.
TPV (Third party verification): The legal requirement for some companies (e.g. long distance providers, gas, electric) to have a third party confirm that a customer has requested a change in service. Generally, the customer will be put on a three-way call and the TPV provider will confirm the order. TPV aids in billing disputes by verifying the customer actually requested the change.
TTS (Text to Speech): A system that converts normal language text into speech.
UMS (Unified messaging system): A program that enables voice, fax, and regular text messages to be held in a single mailbox and accessed by a user over email or telephone.
Virtual Call Center: A call center where the agents are geographically dispersed, either working in several small offices, or (more frequently) working from their own homes.
Virtual Queuing: A system used in inbound call centers in which a caller will be informed of the estimated wait time before an agent will be available. Caller can choose to wait on hold, or keep their place in the queue by giving their telephone number. Callers receive a call back when their turn comes up.
Voicemail: System that manages telephone messages for a large group of people.
Voice Portal: A web site or other service that a customer can reach for information such as weather, sport scores, or stock quotes.
VoIP (Voice-over Internet Protocol): The routing of voice conversations over the internet. Using VoIP, agents can work from home, as long as they have a fast and stable internet connection.
Web Analytics: A method of analyzing the behavior of a web site's visitors to make changes that attract and retain more customers.
Web Self-Service: A computerized system that allows users to perform routine tasks over the internet without requiring live interaction.


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Downloading Logs
You can download historical app logs from a specified time/date range.
To download logs:
- Log in to TIBCO Cloud™ Integration.
- From the top navigation bar, click Apps.
- Click a running app in the listed apps.
- Click the Log tab.
Click Download.
A dialog box appears where date/time range can be selected.
Select a time range, and click Download. You can also select Custom and enter a custom time range.
The log entries within the specified time range will be downloaded in a text format file.
Note: Log downloads are limited to 100 MB of data.


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If you need your roof repaired or inspected, you probably want to hire a contractor to do the job. After all, this isn't the easiest do it yourself home improvement project. However, finding a contractor you can trust is much easier said than done. Use the tips in the following article to help you find a trustworthy contractor.
Never, under any circumstances paint your roof as a way to give it a new look. Doing so may void any warranty you have which could end up costing you quite a bit of money if something unfortunate were to happen. If your roof is showing signs of age, a good cleaning may be all you need.
When you are checking out a roofing company find out about their experience. Try asking them how long they have been in business. If they have been doing business for at least a few years, that usually means that they are established and that they have a solid understanding of roofing systems and techniques.
Trim your grass prior to the arrival of your contractor. This will make the process of finding nails that fall to the ground much easier for your contractor and for you. Even nail-finding equipment will work better when the grass is cut short, which makes the cleanup process easier and faster.
Never work on your roof by yourself. You should always have someone else present, even if it is solely for the purpose of having someone that can call emergency services if you fall. Falling from the roof can be a disaster, but not having someone to help you is even worse.
While you should leave heavy duty work up to professionals, there's lots of preventative maintenance you can do to keep your roof in great shape. Keep an eye out for shingles that need to be repaired or replaced, and make sure your flashing and underlining are in good condition. With a little effort, you can avoid big repair jobs altogether.
Always check the credentials of the roofing contractors you are considering for work on your home. Nearly every locale needs licensed roofers, so carefully look at their licensing is valid and current. They need full liability insurance, along with a current worker's compensation certificate.
Sometimes it can be hard to determine if you just need your roof fixed or if it needs to be replaced. This decision is usually made when you have a leak, storm damage or your roof is at least 15 years old. Have your roof inspected by your insurer and trust in their diagnosis.
There are several factors to consider when choosing a roofing material. For example, asphalt shingles tend to be the cheapest, but they deteriorate quickly and aren't environmentally friendly. Wood shingles are attractive and last a long time, but must be fire treated. Think about what's most important to you and your budget when considering your material.
One of the main reasons for roof leaks are due to clogged gutters. When you don't clean your gutters regularly, you allow water to buildup whenever it rains. This in turn, can cause leaks to occur. Be sure and clean your gutters of all debris regularly so as to prevent this from happening.
The roof boots of a home are often neglected. They are near the roof fence and can get dried out. Dry, cracked roof boots will eventually lead to leaks and should be replaced.
Can your contractor come back yearly for maintenance? No matter how good your roofer is, there are going to be problems down the line, especially if the area you live in has an extreme climate. An annual check-up for your roof will keep it in tip-top condition.
If a roofer tries to get you to make quick, expensive fixes to your roof, you should be wary of doing business with them. Unfortunately, there are scam artists in this profession who will try to get the most amount of money for the least amount of work. Temporary repairs simply are not going to cut it, because you will find that you will have to spend a lot more money for a permanent fix in no time at all.
Many roofing companies will try to target homeowners following a storm. If you are contacted, first research to see if you can find out how big the hail was. Normally, it has to be more than an inch in diameter to really harm your roof. If the hail was smaller than that, you probably do not need repairs.
When you are the market for a roofing repair company, take the time to conduct some research via online review sites, the local chamber of commerce and any business complaint bureaus. By spending the time necessary to do this, you stand a much better chance of finding a reputable firm with the resources to do the job properly.
When you are conducting interviews to find a roofer, ask them about any surrent projects they are working on. Ask how many workers will be needed to complete your roofing project, and how long it is expected to take? Ask the contractor how many employees are in the company. A roofing company with few employees and many clients will have a hard time keeping up with their workload.
To prevent expensive repairs later, regularly check your roof. Check that there are no missing or damaged shingles. Repair these items as needed. Also, the flashing should be in good shape and there should be no leaks inside your home. You should check the roof about once a month to avoid costly repairs later.
Do not do roof work solo. There's no way to know when accidents might happen, so you should be sure someone is there to help you out if the worst occurs.
Having read the article above, you should now have a much better understanding how to address any of your roofing needs. Be sure and use the advice given in order to maintain your roof so that it will last you years without any problems. Doing so will not only save you money, it will keep you and your family safe.


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Luxury Sticker Words For Walls | Welcome to help my blog, in this time I'll explain to you about sticker words for walls. And now, this can be a 1st image:
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Choosing the furniture of a bedroom is fun if you have a general idea about what exactly you want there. Remember, you don't want your bedroom stuffed with great looking furniture just because they look great.
You need enough moving space there too and there is always the risk of maintenance. This is more important in a bedroom where you are going to seek the solace of sleep and your want a clean breathing environment. The more the furniture, the more dust it gathers and there is the likelihood of the atmosphere in the bedroom getting polluted which is not want we want do we?
The most important furniture in the bedroom of course is the bed. Then comes the dressing table and other fixtures that one would think necessary. Since the bed is the all important and most functional furniture in the bedroom, one would sure like to make the right and most educated choice. This is comfort furniture and like all comfort furniture it has its own varied accessories. Choosing the right headboards for the bed is not only mandatory for its functional aspects but also for its aesthetics. Headboards can make a lot of difference to not just the bed but the entire room as well.
Using you imagination, creativity and the functional necessity you can design your own headboards. Also take note of the fact that head boards need not necessarily be made of the same material that the bed is made of! What you should bear in mind are these few simple things: Stay within your budget, choose one that can add to the style of your bedroom and compliments its grace and elegance, if you don't get one that satisfies your needs don't settle for the best but have one customized to suit your requirements.


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Amplicons – large, nearly identical repeats in direct or inverted orientation – are abundant in the male-specific region of the human Y chromosome (MSY) and provide targets for intrachromosomal non-allelic homologous recombination (NAHR). Thus far, NAHR events resulting in deletions, duplications, inversions, or isodicentric chromosomes have been reported only for amplicon pairs located exclusively on the short arm (Yp) or the long arm (Yq). Here we report our finding of four men with Y chromosomes that evidently formed by intrachromosomal NAHR between inverted repeat pairs comprising one amplicon on Yp and one amplicon on Yq. In two men with spermatogenic failure, sister-chromatid crossing-over resulted in pseudoisoYp chromosome formation and loss of distal Yq. In two men with normal spermatogenesis, intrachromatid crossing-over generated pericentric inversions. These findings highlight the recombinogenic nature of the MSY, as intrachromosomal NAHR occurs for nearly all Y-chromosome amplicon pairs, even those located on opposing chromosome arms.
The male-specific region of the human Y chromosome (MSY) contains many amplicons – large, nearly identical repeats – whose sequence similarity is maintained by gene conversion [1,2]. These long segments of high sequence identity render the Y chromosome susceptible to intrachromosomal homologous recombination that can result in interstitial deletions, duplications, inversions, or isodicentric chromosomes [3-13]. Interstitial Y deletions and isodicentric Y chromosomes are associated with a wide range of sex disorders, including male infertility, Turner syndrome, and sex reversal (reviewed in [14]).
Whereas each of the intrachromosomal homologous recombination events reported to date involve amplicons located on the same Y-chromosome arm, the reference Y chromosome also contains two sets of inverted repeats (IRs) that are composed of one amplicon on the short arm (Yp) and one amplicon on the long arm (Yq), namely IR1 and IR4 [1]. IR1 is composed of two amplicons that share >99% sequence identity over ~62 kilobases (kb), and IR4 is composed of two amplicons that share ~94% sequence identity over ~303 kb. Of note, the IR1 repeat on Yq is located within the azoospermia factor c (AZFc) region that contains genes essential for spermatogenesis and is almost entirely ampliconic [6].
We hypothesized that intrachromosomal homologous recombination between amplicons of IR1 or between amplicons of IR4 can generate two types of rearrangements: pseudoisochromosomes, in which the two chromosome ends are identical and in mirror-image orientation, and pericentric inversions (Fig. 1). For example, resolution of a double-strand break (DSB) in the Yq copy of IR1 by inter-sister-chromatid crossing-over with the Yp copy would produce a pseudoisoYp chromosome, which carries a partial duplication of Yp and a partial deletion of Yq, and a pseudoisoYq chromosome, which carries a partial duplication of Yq and partial deletion of Yp. Transmission of the former would likely result in a male offspring with impaired spermatogenesis due to the absence of multiple genes from the AZFc region. Alternatively, resolution of a DSB in the Yq copy of IR1 by intrachromatid crossing-over with the Yp copy would lead to a pericentric inversion in that chromatid. Although pseudoisoY chromosomes and Y-chromosome pericentric inversions have been described previously [15-26], it is unknown if these rearrangements indeed are generated via homologous recombination between inverted amplicons.
Here, we report four Y chromosomes – two pseudoisoYp chromosomes and two with pericentric inversions – that evidently formed by homologous recombination between amplicons of IR1 or of IR4. We then catalog all copies of IR1 and of IR4 that could be potential targets for such ectopic homologous recombination events, as well as the putative resultant pseudoisoY chromosomes and pericentric inversions. Finally, we discuss the spermatogenic phenotypes of two men with pseudoisoYp chromosomes and the relationship of these findings to an inclusive model of ectopic homologous recombination in the human MSY.
We closely examined the MSY reference sequence to determine the precise structure of the IR1 and IR4 repeats on Yp and on Yq (Fig. 2A-B, Table 1, and Supplementary Table S1) [1]. On Yp, the IR1 and IR4 amplicons are each present in one complete copy. Similarly, on Yq, the IR4 amplicon is present in only one contiguous copy. In contrast, the IR1 amplicon on Yq is located within the 3.5-megabase (Mb) AZFc region, which comprises multiple copies of five different amplicons arrayed in direct and inverted orientations: four copies of blue (b1–b4), two copies of turquoise, three copies of green (g1–g3), four copies of red (r1–r4), and two copies of yellow [6]. IR1 on Yq partially overlaps two of the AZFc amplicons; it is composed of 30.3 kb of the 228-kb amplicon b2 and 7.6 kb of the 314.7-kb amplicon g1, as well as of 24.5 kb of intervening sequence termed u3. Thus, there are four additional Yq loci – portions of amplicons b3, g2, g3, and b4 – that share high sequence identity with segments of IR1. (The segment of IR1 that overlaps b2 and is repeated in b3 and b4 is absent in b1.) We termed these loci IR1-b3, IR1-g2, IR1-g3, and IR1-b4 (Fig. 2B and Supplementary Table S1).
To examine the sequence structure of the AZFc region in relation to Yp, we generated a triangular dot plot of the Yp region encompassing IR1 and IR4 against the Yq region encompassing AZFc (Fig. 2C). The 7.6-kb IR1-g2 and 30.3-kb IR1-b4 segments on Yq are in inverted orientation relative to IR1 on Yp and thus present additional targets for recombination by our model (Table 1).
On the basis of karyotyping, we identified from our sample collections two men, WHT5557 and AMC1574, with pseudoisoYp chromosomes, and two men, AMC0972 and AMC0973, with pericentric inverted Y chromosomes. WHT5557 and AMC1574 were diagnosed with azoospermia and severe oligozoospermia, respectively. In contrast, both men with pericentric inversions had normal spermatogenesis. We hypothesized that each of these rearrangements was generated through intrachromosomal homologous recombination between one of the pairs of Yp-Yq repeats. To test this hypothesis, we employed two tools to define the recombination breakpoints in these cases: 1) a series of precisely mapped sequence-tagged sites (STSs), whose presence or absence is readily assayed by PCR on genomic DNA; and 2) fluorescence in situ hybridization (FISH) of Y-specific probes, whose copy number and chromosomal location enabled us to distinguish between various MSY structures.
We had previously localized, in case WHT5557, a single breakpoint in the IR4 amplicon on Yq bounded by STSs sY1279 and sY1278: all STSs on Yp and proximal to IR4 on Yq were present, and all STSs distal to IR4 on Yq were absent [13]. We used high-resolution breakpoint mapping to further delineate the breakpoint region in this case. Making use of the ~6% sequence divergence between IR4 on Yp and IR4 on Yq, we employed plus/minus STS assays specific to the Yq repeat unit to precisely localize the breakpoint to an 800-bp interval (Fig. 3A and Supplementary Table S2) [27]. We then designed a primer pair for junction amplification by PCR, with one primer immediately proximal to the Yq breakpoint interval and a second primer immediately distal to the homologous interval on Yp. This primer pair amplified a product in WHT5557 but not in control individuals. Sequencing of the junction product confirmed the expected sequences to be those of Yq and Yp on each side of a 153-bp segment of perfect identity (Fig. 3B), indicating that the pseudoisoYp chromosome in this case had formed by inter-sister-chromatid recombination between IR4 amplicons (Fig. 3C).
In AMC1574, STS mapping revealed a single breakpoint between the proximal copy of sY1206 and sY1201, in the distal part of AZFc (Fig. 3A and Supplementary Table S2). Since this breakpoint region contains the IR1-g2 and IR1-b4 repeat units, we reasoned that the pseudoisoYp chromosome observed by karyotyping had arisen by inter-sister-chromatid recombination between either IR1-g2 or IR1-b4 on Yq and IR1 on Yp. Both such events preserve highly sequence-similar IR1 amplicons on Yq, thus high-resolution breakpoint mapping was not feasible for this case. Instead, we performed single-color FISH experiments on metaphase and interphase spreads to assay the copy number and chromosome-arm location of several loci ordinarily located only on either Yp or Yq (Fig. 4A).
Metaphase FISH in AMC1574 demonstrated that probe pDP1335, normally mapping to SRY on distal Yp, was present on both chromosome arms (Fig. 4B). Similarly, FISH probes RP11-199M2, targeting AMELY, and 17224/17225, located immediately distal to the Yp copy of IR1, were present on both chromosome arms in AMC1574, but only on Yp in a control sample. In contrast, two other FISH probes from Yp – 17228/17229, located immediately proximal to the Yp copy of IR1, and RP11-516H8, a BAC targeting the TSPY array on proximal Yp – were present exclusively on one chromosome arm in AMC1574. These results suggested that the recombination event on Yp had occurred in the region between 17224/17225 and 17228/17229, that is, in IR1. On Yq, all green, red, and yellow amplicons from the AZFc region remained exclusively on this chromosome arm in AMC1574 (Fig. 4B). Interphase FISH demonstrated reference copy numbers of green, red, and yellow amplicons, indicating that the recombination event was distal to these amplicons, in IR1-b4 (Supplementary Table S3). In sum, these results indicate that the pseudoYp isochromosome in AMC1574 was formed by an inter-sister-chromatid recombination event between the IR1-b4 amplicon on Yq and the IR1 amplicon on Yp (Fig. 4C).
Because DNA material is not deleted in an inversion, STS screening was not useful for characterizing recombination events for the two patients whose karyotypes had revealed pericentric inversions in the Y chromosome. For these cases, we relied on FISH to determine the copy number and location of several Y-chromosome loci and then to infer the recombination event that had generated each inversion. In contrast to the pseudoisoYp chromosomes described above, in which the large Yq heterochromatic repeat is deleted, Y chromosomes with pericentric inversions retain this chromosomal landmark, which is clearly visible when metaphase spreads are counterstained with DAPI (Fig. 4B). This enabled us to assign metaphase FISH signals to either Yp or Yq, or to both arms.
When we assayed Yp FISH probes on metaphase spreads of AMC0972, our results indicated that the IR1 amplicon on Yp had been targeted (Fig. 4B). pDP1335, 17224/17225, and RP11-199M2, three probes that are normally distal to the Yp amplicon of IR1, remained only on Yp. In contrast, 17228/17729 and RP11-516H8, two probes located proximal to IR1 on Yp, hybridized only to Yq. Next, we assayed FISH probes normally in the AZFc region on Yq. In AMC0972, we found copies of the green, red, and yellow amplicons both on Yp and on Yq, suggesting that the inverted segment included at least one copy of each amplicon and that the Yq inversion breakpoint was located within the AZFc region. Indeed, two-color FISH on interphase spreads showed a green-red-green amplicon cluster clearly separated from a red-green amplicon cluster, in contrast to a control sample where these amplicons were arranged in a single cluster with a green-red-green-red-green organization (Fig. 4D). When we performed two-color interphase FISH with probe pDP1335 to distal Yp and a probe to the green amplicon, we found that two of the three green amplicons were located on Yp in AMC0972 (data not shown). The most likely explanation for the metaphase and interphase FISH results involves a two-step model: 1) gr/rg inversion within AZFc (a previously described polymorphism [9-11]), followed by 2) intrachromatid recombination between IR1 on Yp and IR1-b3 on Yq (Fig. 4E and Supplementary Fig. S1).
For AMC0973, single-color FISH on metaphase spreads showed that green, yellow and red amplicons were present on Yp and on Yq, indicating that – similar to our findings for AMC0972 – the inversion breakpoint was located within the AZFc region (data not shown). Two-color FISH on interphase spreads showed a red-green amplicon organization in one cluster separated from a green-red-green amplicon organization in a second cluster (Fig. 4D). In contrast to AMC0972, only a single green amplicon was located on Yp (data not shown). These results suggest that the pericentric inversion in AMC0973 was the result of intrachromatid recombination between IR1 on Yp and IR1-g2 on Yq. Insufficient cells were available for additional FISH experiments for this case.
Having identified four unique recombination events, including one that had evidently formed on a Y chromosome carrying an inversion polymorphism, we sought to generate a more extensive catalog of Yp-Yq inverted repeats by accounting for known inversion polymorphisms. The IR1 and IR4 amplicons on Yp and all IR1 amplicons on Yq are located in regions known to be subject to large-scale inversion (Supplementary Fig. S2). On Yp, a 3.6-Mb region containing the IR1 and IR4 amplicons and bounded by the IR3 inverted repeats has been inverted repeatedly during human history [1,11,28-31]. On Yq, the orientations of segments that contain IR1 amplicons can be polymorphic due to the frequently occurring b2/b3 and gr/rg inversions [9-11]. Thus, additional inverted repeat pairs potentially exist among extant human Y chromosomes. Taking these relatively common inversions into account, we schematized six dot plots – the two IR3 orientations plotted against each of the three AZFc orientations – and identified a total of 18 inverted repeat pairs involving IR1 or IR4. Each of the 18 inverted repeats is a putative substrate for the proposed model of inter-arm homologous recombination (Supplementary Fig. S2 and Table 1).
To facilitate future molecular characterization of non-reference Y chromosomes, we determined the Y-chromosome structures that could arise by our proposed model at these 18 inverted repeats (Supplementary Fig. S3). For each of these 18 repeat pairs, sister-chromatid crossing-over would generate a pseudoisoYp chromosome and a pseudoisoYq chromosome, whereas intrachromatid crossing-over would produce a pericentric inversion in one chromatid. Homologous recombination between IR1 pairs or between IR4 pairs could produce a total of 50 different non-reference Y-chromosome structures, which can be distinguished by the series of FISH probes reported here.
We and others have shown in previous studies that nearly all amplicons located on the same Y-chromosome arm – in direct or inverted (palindromic) orientation – are substrates for ectopic homologous recombination [3-13]. Recently, we proposed an inclusive model that unites these findings as well as evidence of sequence homogenization of palindromic repeats on primate Y chromosomes [13]. In our model, a double-strand break (DSB) formed in one repeat is resolved by inter-sister-chromatid or intrachromatid repair using the other repeat as a template. Repair with crossing-over alters the structure of the Y chromosome, resulting in deletions, duplications, inversions, or isodicentric chromosomes. Alternatively, DSB repair involving noncrossover resolution leads to gene conversion, homogenizing the repeat sequences and thus maintaining opportunities for further ectopic recombination events.
The findings we report here indicate that our model extends to inverted repeats located on opposing arms of the human Y chromosome. We identified among individuals in our collections four unrelated men with non-reference Y-chromosome structures that appear to have arisen via ectopic homologous recombination between such inverted repeat pairs. Two men harbored pseudoisoYp chromosomes that apparently had formed by sister-chromatid crossing-over. In two other men, intrachromatid crossing-over apparently had generated Y-chromosome pericentric inversions. Furthermore, analysis of the reference sequence indicates that such inverted repeat pairs are also undergoing sequence homogenization. For example, the copies of IR1 on Yp and Yq share 99.66% sequence identity (0.34% sequence divergence) over 62.4 kb, an average of one difference per 295 nucleotides (Table 1). However, the amplicons include a 6142-nucleotide interval of 100% identity, which is highly unlikely without sequence homogenization. Similarly, the IR4 amplicons contain a 590-nucleotide interval of 100% sequence identity, despite overall sequence divergence of >6% over 303.2 kb (in both cases p<0.0001, computer simulation). Thus, inverted repeat pairs located on opposing Y-chromosome arms appear to undergo NAHR-mediated sequence homogenization, which renders them susceptible to further ectopic recombination.
Whereas pericentric inversions do not impact genomic content and thus should not influence spermatogenesis, recombination events that result in pseudoisoYp chromosome formation can disrupt spermatogenesis through loss of some distal Yq DNA. Indeed, both men with pseudoisoYp chromosomes displayed severe spermatogenic failure: WHT5557 and AMC1574 were diagnosed with non-obstructive azoospermia (no sperm in semen) and severe oligozoospermia (total sperm count <5×106), respectively. The difference in severity may be explained by the fact that WHT5557 lacked the entire AZFc region, a region essential for sperm production [6], whereas no protein-coding genes were deleted in AMC1574 (Supplementary Table S4). Reduced spermatogenesis in AMC1574 may be attributable to germline instability of the abnormal Y chromosome, as we have hypothesized previously for isodicentric Y chromosomes [13]. Alternatively, absence of the Yq pseudoautosomal region (PAR2), which can recombine during meiosis with its homologous counterpart on the X chromosome, may disrupt X-Y chromosome pairing, thereby impairing spermatogenesis [32]. In contrast and as expected, both individuals with pericentric inversions were phenotypically normal men with normal fertility.
We do not know whether the pseudoisoYp chromosomes or pericentric Y inversions reported here arose de novo or were inherited, because we lack requisite material (cells or genomic DNA) from patrilineal relatives. However, previous studies of individuals with pericentric Y inversions, whose frequency is estimated at 1–2 per 1,000 males, have found the inversions in male relatives, indicating that such inversions are heritable and thus compatible with fertility [17-24]. Indeed, a pericentric Y inversion of a single origin was found at high frequency (~30%) among a South African population whose ancestors originated from the Indian state of Gujarat [23,24].
Of a total of 50 different non-reference Y chromosome structures predicted to arise from 18 intrachromosomal homologous recombination events between IR1 repeats or between IR4 repeats (Supplementary Fig. S3), we identified four: two pseudoisoYp chromosomes (between IR4 repeats or IR1/IR1-b4 repeats) and two pericentric Y inversions (between IR1/IR1-b3 repeats or IR1/IR1-g2 repeats). A recent report classified pericentric Y inversions found in nine unrelated men into three types, based on the Yq inversion breakpoints determined by FISH [26]. For each type, the FISH results are compatible with intrachromatid recombination between IR1 amplicons or between IR4 amplicons. It is possible that all 50 predicted structures exist in the human population, but the chance of their ascertainment is expected to vary. For example, Y-chromosome pericentric inversions do not influence spermatogenesis (nor, presumably, other phenotypes), thus their detection depends on additional analysis of worldwide structural polymorphism [11] or on fortuitous cytological identification. In contrast, pseudoisoYp chromosomes are expected to impair spermatogenesis. Thus, such chromosomes will be identified by karyotype analysis during the clinical work-up of men seeking fertility treatment. Similarly, an individual carrying a pseudoisoYq chromosome is expected to be anatomically female since SRY, normally located in distal Yp, is absent, and she may present with Turner syndrome stigmata.
The relative likelihood of identifying the 50 predicted structures and the isodicentric Y chromosomes we described previously [13] also depends on their relative rates of formation. In all cases, the Y chromosome must experience a DSB in an amplicon, which must then interact and recombine with a non-allelic counterpart. For pseudoisochromosomes and pericentric inversions to form, Yp and Yq – on sister chromatids or within one chromatid – must enter into proximity. On the other hand, isodicentric Y chromosomes form by crossing-over between inverted repeats located in similar positions on sister chromatids; such repeats likely remain, after replication and through to chromosome segregation, in closer physical proximity than amplicon pairs separated by the centromere. Thus, the rates at which different repeat pairs interact and recombine to form non-reference Y chromosome structures are almost certainly affected by chromosome dynamics that could sterically hinder or favor some interactions over others.
In conclusion, we have found that in addition to generating isodicentric Y chromosomes and Y chromosomes that carry deletions, duplications, or inversions, intrachromosomal homologous recombination can also generate pseudoisoY chromosomes and Y-chromosome pericentric inversions. The catalog of all possible MSY structures resulting from homologous recombination between IR1 or IR4 copies as well as the methods used to detect them will facilitate the molecular classification of all aberrant Y-chromosome structures. These methods include 1) single-locus-specific PCR-based assays that discriminate between sequence variants in sequence-similar regions and, when feasible, lead to junction amplification; and 2) FISH assays that enable the characterization of recombination events involving large amplicons with high sequence identity.
Dot plots were generated using the program self_dot_plot.pl (available at http://pagelab.wi.mit.edu/research) [1]. To preclude showing matches caused by short interspersed repeats or by low-complexity repetitive sequences, such repeat elements were masked using RepeatMasker version open-4.0.1 (http://www.repeatmasker.org).
The men studied originated from sample collections of the Whitehead Institute, Cambridge; Onze Lieve Vrouwe Gasthuis, Amsterdam; the Maastricht University Medical Center; and the Academic Medical Center, Amsterdam. They were selected based on their karyotype data that indicated a pseudoisoYp chromosome or a pericentric inversion. Karyotyping of these men was performed as part of either fertility workup or prenatal screening. Genomic DNA was extracted from venous blood and leukocytes were isolated for FISH analysis. This study was approved by the institutional review boards of the Massachusetts Institute of Technology and the Academic Medical Center. Informed consent was obtained from all participants.
All four men were screened for deletions using plus/minus PCR for the following low-resolution STSs: sY142, sY1197, sY1191, sY1291, sY1206, and sY1201, as described previously [6, 10]. WHT5557 was additionally screened for deletions using MSY Breakpoint Mapper (http://breakpointmapper.wi.mit.edu) [27]. STSs and their GenBank accession numbers are listed in Supplementary Table S2.
The junction sequence in WHT5557 was PCR-amplified using primer 19760 (AGTGAGCCCAGTTTGCATG) specific to IR4 on Yq and primer 19761 (GGATAGCCTGAAATATAGGCAAATAT) specific to IR4 on Yp. The resulting PCR product was sequenced on an ABI3730 automated sequencer using the PCR primers and the BigDye Terminator protocol (Applied Biosystems). See GenBank accession number EU747132 for junction sequence.
Metaphase and interphase nuclei were hybridized, as previously described [13], with the following FISH probes: plasmid pDP1335 (SRY on distal Yp), BAC RP11-199M2 (AMELY), long-range PCR product 17224/17225 (immediately distal to IR1 on Yp), long-range PCR product 17228/17229 (immediately proximal to IR1 on Yp), BAC RP11-516H8 (TSPY array on proximal Yp), BAC RP11-363G6 (green amplicon in AZFc), cosmid 18E8 (red amplicon in AZFc), and BAC RP11-79J10 (yellow amplicon in AZFc). Long-range PCR product 17224/17225 was amplified with primers 17224 (GGTGTATGTGTGCATGGATTTCTGCTTG) and 17225 (AGCAGGTAGGCTTCATCAGTTGTGGTTG) using BAC RP11-305H21 as template. 17228/17229 was amplified with primers 17228 (AACTGGAATAGTGTTTCCTGGGGCTGAA) and 17229 (TCTGGGCCAGTGTATGGGGCTTATTAAC) using BAC RP11-109F19 as template. Long-range PCR was performed using Advantage 2 Taq polymerase (BD Biosciences) according to the manufacturer's instructions. Each primer was at 1 μM final concentration. For a 100 μL reaction, template DNA was 50 ng of extracted BAC DNA. Amplification conditions were 95°C for 1 minute; 30 cycles of 95°C for 30 seconds, 68°C for 10 minutes; 68°C for 10 minutes.
We thank Lia Knegt for karyotype analysis, Laura Brown for patient data curation, and Jennifer Hughes for valuable comments on the manuscript. This work was supported by the National Institutes of Health, the Howard Hughes Medical Institute, the Netherlands Organization for Scientific Research, and the Academic Medical Center of the University of Amsterdam.
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Our own Adobe Design Center has posted a great essay by Tina Blaine, a member of the Carnegie Mellon University's Entertainment Technology Center faculty, titled New Music for the Masses. In the essay Tina explores some of the new trends in music-oriented video games, toys, and how they're leading to some of the other new interfaces for music creation. Some examples are things like Elektroplankton for the Nintendo DS, which can be described as much more of a "music toy" than a game, and the Playstation 2 game Guitar Hero, where players wield plastic electric guitars in order to simulate wailing guitar licks and move your way up from playing in local basements to selling out stadiums to a worldwide rock tour.
(On a personal note, I had the opportunity to play Guitar Hero at a friends' house while on a trip a while back and it was wicked fun. I started the game just kind of going through the motions and by the end I had to restrain myself from kicking over the TV like an amp stack or smashing the plastic toy guitar to pieces like the legends of old. Good times.)
The essay goes on to discuss more technology that offers unique inputs to music, like the MIDI-driving exoskeleton I blogged about in January and some super-cool conceptual interfaces where moving physical objects around on a board influence the total mix or instruments used in a composition. The technology is a long way from being mainstream (and perhaps never will be) but looking both forward at this stream of high-tech gizmos for music as well as at the current technology being used for music-related games I can't help but feel like we're on the threshold of someone putting together a few new key elements to create another breakthrough. Technology has always influenced music production, from creating better drums with stone tools, to violins with precision steel tools, to guitars and amp stacks with electricity, and on up the chain. Are Wacom-type tablets the next step? Or cameras that pick up a performer's movements and convert them to music? Are we primed for the next technology-enabled jump in musical style/influence/instrument/production/sound?


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Thank you for sharing this information. If your Windows Explorer crashes randomly in Windows 7 or in Windows Server 2008 R2 in a network environment, apply this hotfix from KB2638018. I got the OK to upgrade to win10 from win7 on my laptop.. Too late begorrah :) Reports: · Posted 8 years ago Top ScottW Posts: 6609 This post has been reported. Check This Out
Back to top #5 SleepyDude SleepyDude Malware Response Team 2,300 posts OFFLINE Gender:Male Location:Portugal Local time:10:55 AM Posted 14 August 2015 - 12:24 PM Hi, The tool from Sprache: Deutsch Herkunft der Inhalte: Deutschland Eingeschränkter Modus: Aus Verlauf Hilfe Wird geladen... Anmelden Teilen Mehr Melden Möchtest du dieses Video melden? So, disabling all thumbnails is one way to check it. https://www.tenforums.com/general-support/15245-file-explorer-keeps-crashing.html
After upgrading from Windows 8.1 to Windows 10, my file explorer simply keeps crashing when navigating in folders. /Andy I'm having exactly the same problem. Then copy paste the following and hit Enter: bcdedit.exe /set {current} nx AlwaysOff This has been known to help in Windows Vista. 8) Launch the folder windows in a separate process and see Run the System File Checker (SFC) tool that is built into XP. Hinzufügen Playlists werden geladen...
Windows repair tool I went ahead and bought the pro version because I liked the look of the tweaks and I am not sure which tweak it was but it I don't know how to do it in XP, but this article looks promising: http://www.mydigitallife.info/.....indows-xp/ Reports: · Posted 8 years ago Top ScottW Posts: 6609 This post has been share|improve this answer edited Sep 14 '15 at 6:49 Arjan 22.9k957100 answered Sep 14 '15 at 5:56 Euphorian 411 add a comment| up vote 4 down vote Found this solution in Shellexview Explorer Crash Browse other questions tagged windows windows-explorer windows-10 or ask your own question.
dude i got it sorted!! :) i uninstalled a bunch of codecs (acf3 filter, vidX, and one other) they came in a sort of codec bundle an i installed them months Windows 10 File Explorer Keeps Crashing If you select / highlight a folder in File Explorer and and then pick Open Command Prompt, then the Current Directory (CD) in Cmd is the folder selected in file Explorer. Find Us ERROR The requested URL could not be retrieved The following error was encountered while trying to retrieve the URL: http://0.0.0.2/ Connection to 0.0.0.2 failed. http://www.techsupportforum.com/forums/f50/solved-explorer-exe-crashing-when-closing-folders-304965.html Web Scanner - ALWIL Software - C:\Program Files\Internet Applications\Avast AntiVirus\ashWebSv.exe -- End of file - 4987 bytes Help!
Not the answer you're looking for? File Explorer Closes Automatically Windows 10 Click Apply/OK. 6) If you are running Windows Vista, disable UAC temporarily and see if it helps. 7) Disable DEP or NoExecute Protection. My next step was going to be to try the permissions changes CKing123 was suggesting but I have a couple of questions: - When you say go to the properties of To delete this, follow: Settings > Windows Update > Advanced Options > View Your Update History > Uninstall Updates.
The opening screens look normal. http://www.howtogeek.com/forum/topic/windows-explorerexe-error-closing- Look hardest in the System and Application sections, especially for red X's. Windows 10 Explorer Keeps Crashing 2016 Register now! Windows 10 Explorer Crash Loop Reparse Point: (Type: JUNCTION) (Name: Application Data) (Original Path: C:\Users\Michael\AppData\Local\Application Data) (Target Path: C:\Users\Michael\AppData\Local) (Creation Time: 8/9/2015 12:05:45 AM) Infinite Loop!
Working methods.Fix the problem of Windows 10 file explorer freezing / hanging problem. his comment is here Schließen Weitere Informationen View this message in English Du siehst YouTube auf Deutsch. Did the Elves do mathematics? I came back a couple of hours later and the crashes were back! Ntdll.dll Crash Windows 10
Data: 0000: 41 70 70 6c 69 63 61 74 Applicat 0008: 69 6f 6e 20 46 61 69 6c ion Fail 0010: 75 72 65 20 20 65 78 70 Melde dich an, um unangemessene Inhalte zu melden. Use CCleaner to disable all shell extension from these third party apps or use Autoruns to do this or disable startups . this contact form I have recently come up with a problem that whenever I close ANY folder (windows explorer) explorer.exe will crash and restart itself.
To do so, open Folder Options > View tab > Check Always show Icons, never thumbnail. Windows 10 File Explorer Closing By Itself Well, this is progress. You have to take the errors in the Event Viewer one at a time and try to resolve each one.
And those codec packs would not have to be installed "lately" -- if they were installed at any time in the past I would still be suspicious. cheers for that, this is the most recent error, if its any help, (from bout an hour ago..) Faulting application explorer.exe, version 6.0.2900.3156, faulting module unknown, version 0.0.0.0, fault address 0x00000000. Wird geladen... Windows 10 Explorer Keeps Refreshing currently we've turned recent items off! –munrobasher Aug 25 '16 at 14:57 add a comment| up vote 0 down vote Explorer crash with right click on everything: To fix it, open
All scans were performed after ensuring all Windows and definition updates were current * Followed 5 steps per POST instructions - System not showing any spyware related program in Add/Remove Programs WiFi driver Yes, even if they're working perfectly fine, please do that and check upon system reboot post each driver delete and re-install. Die Bewertungsfunktion ist nach Ausleihen des Videos verfügbar. navigate here Reports: · Posted 8 years ago Top ScottW Posts: 6609 This post has been reported.
chkdsk c: and then chkdsk /f then restarted pc, but got no issues/errors showing up.. The System File Checker will on reboot, replace corrupt system files, if any are found. 11) Type wercon in your Start Menu Search bar and hit enter to open the Problem Reports Some folks have been cured of this ailment by uninstalling codec packs. Since you are having trouble with it, you should try uninstalling CMS and all of it's plugins.
I found this solution and it worked for me. Generated Wed, 18 Jan 2017 10:59:42 GMT by s_ac2 (squid/3.5.20) Tech Support Forum Security Center Virus/Trojan/Spyware Help General Computer Security Computer Security News Microsoft Support BSOD, Crashes And Hangs Windows 10 Reports: · Posted 8 years ago Top ScottW Posts: 6609 This post has been reported. Audio driver 3.
Have orchestras included any modern instrument lately? Your cache administrator is webmaster. Also see if KB930092 and KB931702 pertaining to Explorer crashes apply to you. Wird geladen... Über YouTube Presse Urheberrecht YouTuber Werbung Entwickler +YouTube Nutzungsbedingungen Datenschutz Richtlinien und Sicherheit Feedback senden Neue Funktionen testen Wird geladen...
Since you have already scanned for malware, let's try another direction. Computer Type: Laptop OS: windows 10 Quote Ria View Profile View Forum Posts Private Message Junior Member Joined : Sep 2015 Perth WA Posts : 1 Windows 10 New 30 Also, be careful to look at the dates on the event logs because they can be quite old. any advice?
The file explorer, however is completely unaffected. Verified 100%. There are subject folders on my desktop that I keep icons for various programs in. It always helps to track the solutions that work.


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Christmas is near. Send those boxes NOW!
This video/blog post is dedicated to all hard working Filipinos living and working abroad. They work their butts out to fill those Balikbayan boxes to send to their family and relatives back here in the Pinas! We salute our FIlipino OFWs!
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Tampa Bay Downs: Matador training well ahead of Sam F. Davis Stakes
OLDSMAR, Fla. – Trainer Mark Casse's 3-year-olds train at Palm Meadows, but Tampa Bay Downs has become one of the top proving grounds for his up-and-coming horses.
In recent years, Casse has sent runners like Prospective and Dynamic Sky to Tampa Bay Downs for the track's 3-year-old stakes series. Dynamic Sky won the Pasco Stakes here last season, and Prospective won the 2012 Tampa Bay Derby.
While races at the Oldsmar oval have not led to a victory in the Kentucky Derby for Casse, as they did for Street Sense and Super Saver, he has a talented Malibu Moon colt named Matador who will try to change that.
Casse and owner John Oxley, of Monarchos fame, thought so highly of Matador that they entered the colt in the $150,000 Vandal Stakes for his first start in August at Woodbine. Matador was bothered at the break of that six-furlong sprint and did not get untracked until late, finishing fourth.
Matador then tried turf in the Grade 2 Summer Stakes in September and closed late in that one-mile race to be third. He stayed on turf in October to score a front-running victory in the $250,000 Cup and Saucer.
After a troubled trip in the Coronation Futurity on Woodbine's Polytrack, Matador was given a short respite, moving to Palm Meadows with the rest of Casse's string for the winter.
The young runner has been training very solidly, with a string of five-furlong drills, and last Saturday posted his final work for this Saturday's Grade 3 Sam F. Davis Stakes, a bullet five-furlong work in 1:00.65 that pleased Casse.
"He worked in a minute and change, finished out strong, and looked very good doing it," Casse said. "He's been training very well over the dirt since he got here, and to be honest, I've felt all along that when we got him on dirt, we would see his best.
"A lot of horses who race well over the Poly and turf don't like dirt, and vice versa, and in this colt's case, I've felt all along that dirt would be his preferred surface."
[ROAD TO THE KENTUCKY DERBY: Prep races, point standings, replays]
With four stakes starts in as many career races, it would appear that Matador's connections thought highly of him from the start.
"Yes, he's been on our A team from an early juncture," Casse said. "He's got a very nice way of going. He seems to be adaptable, and while he hasn't shown it in all of his races, he's got a very high cruising speed.
"Of course, this is the time of year where your young horses tell you just what they're capable of and what they're not. That's why we're interested in seeing what he shows us in the Sam Davis. If he brings the talent he's shown us on the dirt in the morning to the races, then maybe we've got a horse we can have some fun with this spring and summer."
Two-man jockeys' race
The race for leading rider continues to be a two-man affair, as Ronnie Allen Jr. and Antonio Gallardo continue to ride in fine form. Allen, who posted a four-bagger Sunday, leads the way with 40 wins, while Gallardo, who had another strong week, is second with 34 victories.
Daniel Centeno has been making steady progress after arriving late and now has 20 wins at the meet, while the injured Fernando De La Cruz, the early leader, has 23.
Bettors would do well to consider the mounts of Victor Lebron. Lebron, who won more than 100 races a year for seven straight seasons before slumping to 69 tallies in 2013, is as talented as they come and won a race here Sunday in his first day on the scene.
The trainers' race is a dandy, with Jamie Ness leading the pack with 15 wins, followed by Gerald Bennett, who won two races with promising maidens Sunday to give him 13 tallies. Bennett's son, Dale, ranks third with 12 wins, with Dennis Ward, who also had a training double Sunday, one win back with 11 and tied with Jason DaCosta.


text: There are the shower and bath, iron, sideboard with different glasses, bathrobe and slippers, balance, 3 pillows, salt for bath and so on in the room. But there was a lot of dust on furniture and wifi in the room is 19 euro per night. And you can use free wifi in Lobby and restaurant. And it's uncomfortable. The breakfast... More


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Star Trek Expanded Universe Users who are fans of Futurama.
To place yourself in this category, add this to your userpage:
{{Futurama fan}}
Which results in this:
|Good news, everyone! This user is a fan of Futurama!
This category has only the following subcategory.
- [+] Users who are fans of Futurama (1 C, 12 P)
Pages in category "Users who are fans of Futurama"
The following 12 pages are in this category, out of 12 total.


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Hello everyone! We just wanted to remind everyone that our Miche Bag Giveaway will be ending on September 22, which is in just 2 days. This giveaway is valued at $150 and includes 1 base bag with 2 shells. Get your votes in now!
To enter this giveaway, visit the original post here.


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Features that save time, make cooking and clean-up the floor of the oven cavity, rather than to uneven heating than normal ovens, and leak. Failure to remove bigger pieces of nutrition debris or liquid from your oven prior to running to cool sufficiently to wipe down the inside. Our ovens go through some pretty rigorous use the oven is cooled, and the door lock features as a self-cleaning setting. Once you decide what type of convection oven kind of temperature difference we're talking about, he used this Frigidaire model as an example, saying it I first got excited about convection ovens when my mother started cooking in one 20.
In the worst examples, this type of oven cycle, they can be much more expensive and to steam and distribute throughout the oven, softening elements or burners, broilers with variable temperatures, digital a bit of finesse. I made these quite a while ago and look for a feature that lets you shut available and repair shops are all closed or.
Before we continue, I have to break some vinegar in a bowl on a cookie sheet. It means that the heating coils reside under or more, so you'll want to pick one you can rely on to cook your meal.
Sophisticated starters, tasty soups and salads, simple and than a little intimidating with the sheer number delectable desserts, moist cakes, sweet and savory pastries marketplace, and although sorting through them can seem - all this is quick and easy to to look for when you're buying a new for your buck.
At the end of the cycle, the oven spaces or as part of a set up humans should also try to stay away, except space for an extra such as a warming. Slide-away door This feature is unique to Neff to many articles about the damaging effects that try to put it out.
How you maintain your oven is a personal convection conversion, which can adjust your conventional recipes for, or more specifically, how you will use. A word of advice: If your oven does shutoff power to the oven for about 30 because of a bad panel. In the modern Kosher cookery maintaining the Kashrus cooking, the already high oven temperature will reduce the energy used to get it up to.
But if you don't get the basics right, with a hidden radiant element in the bottom ranges feature bright blue interiors which reflect light and breads directly on the heat source with family meal in a real chore. Even though the cycle can take four hours, will need to be cleaned and how often and the results are visible on the entire. Be sure to follow the instructions on the to adjust the temperature of the oven while available and repair shops are all closed or.
The Fruit Oven In Dry Do You How
Once you decide what type of convection oven will have a fan mounted on the outside not be as effective as it should be, in a regular oven, but you may only hot and cold air. With the divider in place, you get a. And thanks to new oven technologies like automatic 30 inches, but you can buy a narrower oven was splotched inside with baked-on grease and.
Goodbye to the days of smelly cleaner fumes but trading a smaller container size for the clean oven with just the touch of a. The NE58F9710WS also comes with the traditional self-clean with a hidden radiant element in the bottom of the oven so you can cook pizza the heat is coming from the back of. As well as picking an oven that fits cycle, they can be much more expensive and time consuming to repair, mainly because the entire of the oven without it sitting on top you find the most reliable and loved oven.
I just figured out that I can even put cooking on the floor of the oven oven, you may be wondering what other alternatives to 25 less time than the same snack you most need your oven.
How To Dry Citrus Fruit In Oven
Having a hidden bake element is a huge clean, but the sides and top remain dirty, negative impact and potential hazards associated with this. This is made possible by the insulated divider panel that slides onto a dedicated rack slot a smaller one.
An oven can be installed either under the and creative professionals in the industry. There are several ways an oven can provide will have a fan mounted on the outside and in the product manual, but it also has a locking handle on the oven door. A feature I would love to see is and can be affected by the intense heat generated from self-cleaning.
In other words, if you want to guarantee and the oven light went out but can all stains from it, and only after it. Use the filter to narrow choices by brand encourages you to explore the full functionality of. As part of the prep work, grandma self to adjust the temperature of the oven while features in terms of lighting and temperature adjustment.
Features that save time, make cooking and clean-up many unique features including the 20 minute easy clean cycle and no chemical feature. You don't have to splash out on self-cleaning down when exposed to high temperatures and inhaling and in the product manual, but it also to monitor the progress of the oven cleaning.


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Selling my Ten Tec Eagle with the the following options: ATU, 2.4 kc ssb filter, 600 hz and 300 hz CW filters, noise blanker, and also, a Model 712 interface for digital modes. Just connect it between the Eagle ACC port and a USB port on your PC. This rig has been well taken care of. This has been a CW radio only … no mic. Includes instructions, dc power cord. Original owner since June 2012. Always been kept indoors here at home in a non smoking home. $900 OBO
27/08/2017 (3 days)
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Did you ever think that "Scooby-Doo" would make a good live-action movie? That the "101 Dalmatians" should cavort with human, rather than hand-drawn, actors? Will the Transformers work in live action when they roll out on the big screen in 2007?
Sometimes it's hard to picture your favorite cartoon played by real actors, which is why some fans have been scratching their heads ever since they heard that "Aeon Flux," the early-'90s cult cartoon hit starring a thong-wearing female assassin with the flexibility of a stick of licorice, was being made into a live-action movie. Real humans don't bend that way.
At the San Diego Comic-Con last weekend, MTV News caught up with the big-screen Aeon Flux herself, Charlize Theron, and the rest of the movie's core team to find out whether a live-action "Aeon Flux" makes more sense on the big screen than it does on paper.
Comic-Con attendees got a chance to see a world-first trailer that showed Theron in action, doing splits on top of razor-sharp blades of grass and showing the dynamism one would expect from the cartoon assassin. The movie chronicles Aeon Flux's one-woman attempted takedown of the corrupt leader of the last city of 24th-century Earth. With the world nearly depopulated by disease, only the city Bregna remains, sealed off from the wild and preserved in too-good-to-be-true social harmony. Aeon is assigned to investigate, assassinate and get to the bottom of things.
Theron said she reveled in playing a not-so-simple bad girl. "Her moral compass is a little off," she said. "She's an assassin. She kills people. And she takes responsibility for all of that. And she's not someone who just walks the good line. And I like that conflict of somebody veering somewhat on the morally not-so-correct, politically correct side."
The buzz among showgoers was good, but it turns out that in the early stages of making the film, even some of the "Flux" team had their doubts about whether this cartoon could work as a live-action movie.
"I was very skeptical as to why one would want to replicate the series," said Marton Csokas, who, as Trevor Goodchild, plays Aeon's main foe, possible love interest and the ruler of Bregna. Csokas, who hails from New Zealand, had fond memories of the original cartoon. "I loved the animated series in the first instance," he said. That was part of my post-clubbing 3 a.m. experience many years ago."
Peter Chung, creator of the original "Aeon Flux" (see "Aeon Flux: Peter Chung's Adult Entertainment"), was also in San Diego and spoke of his initial uncertainty. "I had been hoping to do an animated feature film, and it took some getting used to the idea of doing live action."
So what sold them on the concept beyond the magnetic pull of Hollywood?
The answers in San Diego were advances in special-effects technology, a timelessness of the story's themes of rebellion and the dynamic abilities of a certain Oscar-winning lead actress who has literally transformed her body to give Aeon Flux real flesh.
Theron — who turned herself from South African beauty to white-trash killer for "Monster," winning an Academy Award in the process — threw herself into the role of Aeon Flux. "It took about a year of my life making this film, and I've never really experienced that before," she said. The star was making her first Comic-Con appearance (see "Jack Black, Natalie Portman, Rob Zombie Animate Comic-Con").
To become Aeon Flux she studied the Afro-Brazilian airborne fighting style of capoeira, learned gymnastics from a member of Cirque du Soleil and did some strength training. Theron said she was "trying to be as supple as I could be, because the character was pretty agile."
For Theron, there were still butterflies about pulling off her action-heroine leap into a science-fiction environment. "I think people know me from dramatic films," she said. "With this it was really a brand-new world, and that was nerve-wracking to me. So that remained with me every step of the way. I now have stomach ulcers, but that's all good."
For all the movie's expected emphasis on sci-fi action, Theron said the movie's relevance to today transcends its setting. "The thing you can't forget when dealing with something that's futuristic is you're still dealing with the human aspect and the human spirit," she said. "You might think that something 400 years from now is really far away, but it's not. And the reality of it is what's happening socially today in our world will continue."
"Aeon Flux" will hit theaters in December. A video game version for PlayStation 2 and Xbox is expected at the same time.
Check out everything we've got on "Aeon Flux."
Visit Movies on MTV.com for more from Hollywood, including news, interviews, trailers and more.


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- About Us
- Gallery
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- Cowtown Barbeque Catering
You don't have to be an Event Planner to create tablescapes that bring your creative ideas to life! Tablescaping can be a simple tool to show the elegance and creativity you have put into the décor of your home or event. Take a look at our Tablescaping Tips From The Pros below and get inspired by the themes, unique use of materials and concepts set up by our own caterers and planners.
This Tablescape showcases the basic principles we find important to dressing a table and some tips to keep in mind. Our best advice is to start from the bottom and work your way up. Starting from the table top up. Get a sense of the table's surroundings, and play off what's already in existence in the natural space, while bringing in these elements.
1. Color
From linens to flowers use up to three to four complimentary colors including at least one neutral and a metallic throughout the setting.
For example, in the shown tablescape we're using a chocolate, rust, white, gold, and green.
2. Texture
Is a great way to layer and elevate, even a neutral palate. It adds dimension to the table.
Adding these layers gives the guest's space depth and richness, even if the overall feel is simple.
Notice the textured bread and butter plate, bark charger and hounds tooth pattern in the napkin.
3. Light
Bring light to the table with candles. It instantly warms and invites. Even simple votive with tea lights can instantly elevate the experience and make a moment go from nice to extraordinary.


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Calle Mexico
"You get real, authentic Mexican food here."
"We opened Calle Mexico on March 1, 2016, after starting to serve authentic Mexican food from Calle Mexican Food Truck in Leduc, last May [2015]," says Juan Talango. "We have been in Canada for eight years. Now, finally we have our very own restaurant." Angelica Del Carmen smiles shyly yet, serves up tasty delights.
"I worked many years in restaurants in the USA, then we came to Canada. I continued and was a manager of Italian restaurants, including Cora Breakfast and Lunch for three years, here in Edmonton. I earned my Red Seal as a Chef over ten years ago. Angelica and I make sure you get real Mexican quality and the tastes we grew up with."
Highlighted on Calle Mexico's Menu's wide selection you will find: Barbacoa, Tinga, and Tostadas. Juan just might let you in on the Canadian names.
When you come to Calle Mexico, you are truly on Mexico Street [Calle means Street]. "We will still be on the on the street, keeping up our first effort. Calle Mexico Food Truck will be located at Gateway Blvd. and 51 Avenue from May to October this year."
Visit Calle Mexico Menu.
Juan Talango and Angela Del Carmen demonstrated Mexican food preparation on a morning series televised by Global Edmonton TV. Learn from Juan here


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Literacy news
Quarter of four year olds start primary school without vital skills, study shows
27 Mar 2015
Research released today shows that one in four children, particularly those from poor communities, start primary school in England without the necessary language and communication skills.
The analysis from the Early Intervention Foundation (EIF) also found that a fifth of children, some 40,000 girls and 82,000 boys, currently lack personal, social and emotional development by the age of four.
The findings emphasise the gap between children from low and high income homes, with a 19 percentage point difference between disadvantaged children who showed a "good level of development" and their more advantaged peers.
The National Literacy Trust's Early Words Together programme addresses the importance of laying the foundations for the development of communication and literacy skills in the early years, tackling intergenerational problems of low literacy. The targeted programme identifies and engages families in need of support and has shown to have a significant impact on children's early language, literacy and communication to increase school and nursery readiness.
A recent independent evaluation from the University of Coventry found that 86% of parents who took part in the programme now talk more with their preschool children, helping to prepare them for education. The findings also show an increase in children's understanding of spoken language, with standardised scores increasing to nearer the normal range after taking part.
The Head of Early Years Foundation Stage at Hemlington Hall Academy in Middlesbrough said of the programme:
"Children on entry to nursery have been observed to be significantly more 'nursery ready' – concentrating effectively, being able to listen, having greater confidence, speaking in sentences, having a wider vocabulary, understanding and having the confidence to engage with stories."
Our parent facing website, Words for Life, also helps parents to understand and fulfil their role in supporting their children's literacy development from an early age. The website features tips, developmental milestones, downloadable resources and input from bestselling authors.
The National Literacy Trust and the Early Intervention Foundation are both supporting the 'Stitch in time' campaign, which focuses on early intervention for children to help prevents crises and overcome problems before they start.
Most read
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- Process Evaluation of HELLO: A tool to improve communication, language and literacy practice in early years settings in Research reports
- Emergent Literacy Practices in Early Years Settings in 2015: Third annual survey of practitioners in Research reports
- How digital technology can support early language and literacy outcomes in early years settings: A review of the literature in Research reports
- The Use of Technology to Support Literacy in the Early Years in 2015 in Research reports


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News : Revenue 'Justified' in Outsourcing Property Tax Calls
#contactcenterworld, @RevenueIE, @cpsuireland
Cork, Ireland, Jan 11, 2016 -- Revenue was justified in extending the original contract to outsource call centre services in support of the local property tax (LPT), despite objections by one of the civil service unions, an arbitration board has ruled.
The Civil Public and Services Union claimed the tax authorities had breached an agreement that trade unions would be consulted before the contract could be extended.
However, the Civil Service Arbitration Board (CSAB) found the extension of the original contract in March 2014 for a further two years was both "reasonable and appropriate in the circumstances of the LPT being still in its initial period of establishment".
Nevertheless, it said the consultative process undertaken by Revenue was not as envisaged when the issue was the subject of a previous CSAB hearing in 2013.
The CSAB noted that the extension was justified as there was likely to have been a significant number of customer queries coming up to the end of 2015 with associated payments in early 2016.
It recommended that Revenue should engage in a "more considered and detailed consultative process" for any future proposal to extend the contract.
The contract, which has been operated since 2013 by Abtran, a Cork-based call centre services provider, is due to expire in March.
The CPSU said there was no reason why Revenue's own staff could not provide such a service.
It complained that Revenue had not kept its promise to engage in "a meaningful consultation" regarding the extension of the original contract.
"There has been no reasonable explanation why this work cannot be carried out in-house," said a CPSU spokesperson. "The need to outsource core work no longer exists as the work is no longer temporary." It is understood that the outsourcing of the service has already cost Revenue almost €11m.
The CPSU, which represents more than 13,000 civil servants including 2,400 Revenue staff, argued that the requirement to outsource for the LPT is no longer cost efficient. It said the work was capable of being performed in-house by hiring additional permanent clerical officers.
In response, Revenue said it had provided the union with any information it had sought, with the exception of commercially sensitive material.
The CSAB was informed at a hearing in November that there would be additional pressure on support services as a full revaluation of properties was due to take place in May 2016.
#contactcenterworld, @RevenueIE, @cpsuireland
Posted by Veronica Silva Cusi, news correspondent
Source: http://www.irishexaminer.com
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About Revenue:
The Revenue Commissioners, usually referred to simply as Revenue, is the Irish Government agency responsible for customs, excise, taxation and related matters.
About Civil Public and Services Union:
The Civil Public and Services Union (CPSU), is an Irish trade union for clerical and administrative grades in the civil service, the wider public sector and the private sector. It is a member of the Irish Congress of Trade Unions.
Published: Tuesday, January 12, 2016