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Posted by CandyMafia on September 14, 2014
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Interested in becoming a lifeguard?
The course provides the knowledge & skills needed to become a certified senior lifeguard. Individuals must attend all scheduled class days and have total participation. Students must pass both the written & physical tests for national YMCA certification.
Lifeguard Class 2017
Next lifeguard certification class is June 6-11, 2017. Must attend all sessions (total of 30 hours) on the following days:
June 6, 7, 8, & 9: 5 pm-9 pm
June 10: 8 am-5 pm
June 11: 1 pm-5 pm.
Must meet minimum qualifications (below).
- YMCA members $200
- Non-member $220
If you are interested in this class see Lou Bergen, Aquatics Coordinator, 843-522-9622.
CPR Lifeguard Registration Form June 2017
Essential Prerequisites: minimum age 16 and must be a very strong swimmer.
Individual must be able to pass a ¼ mile swimming test using various strokes, survival floating, treading water. CPR (adult, child, & infant), First Aid, AED and O2 certification preferred prior to class.
**CPR/First Aid Class will be included at no cost if student is not currently certified. The CPR class will be in addition to the regularly scheduled lifeguard courses.
CPR/First Aid Class
Our next class is June 24, 2017 from 8-12 pm. Please contact Lou Bergen, Aquatics Coordinator to register for this class. Space is limited!
Y member: $20
Non-member: $30


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I hope you're praising God for all that you plan to enjoy this Thanksgiving -- a warm cozy home, a nourishing and satisfying meal, laughter and fellowship, family and friends, break from routines, new memories in the making. I hope you thank Him specifically for all the blessings of everyday comfort and happiness, and all the intangible joys of this life. In the midst of all that joyful gratitude, take some time to meditate on God alone. Praise Him for who He is at the very core of His being -- aside from all the tangible things He has done for you -- and exalt Him for His sake alone. Praise Him because He is GOD, and greater than we can comprehend. Praise Him because He is KING [...Read More]
What Better Reason to Give Thanks?
Choosing Gratitude: Chapter 6
Welcome to the Choosing Gratitude study! If you're not sure what this is all about, we're studying through Choosing Gratitude by Nancy Leigh DeMoss. See the Introduction for more details. How Can I Say Thanks? We're finally getting to the heart of having a gratitude attitude -- how can we make it happen? Here are some practical steps we can take to make it an actual lifestyle. 1. Speak Up Tell people when you're thankful for them, or for something they've done! If you don't voice it, they don't know. For example, thank your pastor when he preaches an exceptionally encouraging or convicting message -- he won't know it was effective, if you don't say anything. Or thank that neighbor [...Read More]
He Who Keeps You
When you feel hopeless or helpless... When you feel utterly alone... When your heart breaks... When you shrink under fear... When you almost drown in grief... When you fall down again and again... When you can't see the light... When you're blinded by the pain... When you need courage, refuge, hope... Look to Jesus. He is your creator, your light, your shield. He who keeps you will never falter. He will cherish you in His everlasting arms. Know someone who needs this comfort today? Tweet about it! Psalm 121, Psalm 84:11 [...Read More]
And Again I Say, Rejoice!
I love catching glimpses of unifying threads between the Old and New Testaments. Like seeing pictures of Christ in the historical and prophetic books. Or realizing Israel's significance in the Pauline epistles. Or seeing God's overarching plan of redemption all the way from Genesis through Revelation. And lately, I've been noticing another common thread -- the command to rejoice. As New Testament Christians, we have so many reasons to rejoice! We know the more blessed side of the cross -- Israel merely had a promise that the full atonement would someday take place, but we can look back and see how God brought it all together. We have the complete canon of Scripture. We have centuries [...Read More]
Comfort from Psalm 63
If you need extra encouragement today, spend some time in Psalm 63. Meditate on it. Pray it back to God. Let it be your heart's cry to your Savior. Cling to Him. And find comfort in the shadow of His wings. Read Psalm 63 Share with me! What's your favorite Psalm of comfort? Want more comfort? Read God's Word with me this week: Tuesday: Judges 1-3, Psalm 69:1-18 Wednesday: Judges 4-6, Psalm 69:19-36 Thursday: Judges 7-9, Psalm 70 Friday: Judges 10-12, Psalm 71 Saturday: Judges 13-15, Proverbs 18 Sunday: reflection or catch-up day Monday: Judges 16-18, Proverbs 19:1-14 [...Read More]
Your Very Own Shepherd
I've always loved Psalm 23. There's so much comfort packed into 6 little verses. It's beautiful and reassuring, but it also reminds us where our focus should be. It puts this life into perspective. The LORD is your shepherd. The one who spoke our universe into existence, the great King of Kings and Lord of Lords, who is holy and awesome and mighty and powerful -- HE is YOUR very own shepherd! He leads you, and guides you, and cares tenderly for you. Isaiah 40:11 tells us that "He will tend his flock like a shepherd; he will gather the lambs in his arms; he will carry them in his bosom, and gently lead those that are with young." Isn't that a tender picture? And it's [...Read More]
Snapshot of a Blessed Person
The Bible reading plan I'm working through includes a Psalm for almost every day. I love that. It's so great to read a big chunk of history or prophecy, and then flip over to read a chapter of devotional poetry. The Psalms are so encouraging and comforting -- and yet they're more than just "greeting card" sentiments. They are jam-packed with theology! For instance, we find chapters about the inerrancy of God's Word (Psalm 19, 119), the creation of man by the direct act of God (Psalm 139), the presence and personality of the Holy Spirit (Psalm 51, 104), and the immutability of God and His various attributes. Psalms speaks a lot about God's standard for righteousness, in particular. [...Read More]
Focus on Gratitude: With All Your Being
Right about now is when New Year's resolutions start to flounder. It's been a few weeks... maybe you started off strong and enthusiastic, but you aren't quite seeing any results yet. Or maybe life is getting too busy again, and your "need-to's" are over-powering your "want-to's." Sound about right? One of my resolutions goals for this year is to focus on gratitude. In fact, I jumped on the "one word" bandwagon and made it my keyword for 2013. I want to be more grateful. I want to have a heart of thankfulness... in all things and for all things. I want that to define me -- just like being a Christian and a wife defines me. But life has gotten pretty busy again (not that it ever [...Read More]
God Is Still Watching (#TTB13)
It's hard to watch the news sometimes. So many reports of violent shootings, rapes, and murders; of men and women committing wicked acts against innocent loved ones; corruption in even the highest levels of our government; lies and greediness and just plain old sin. The past few months especially have been inundated with violence and rampant wickedness. We live in a world overrun with godlessness. And it's only getting worse... But you know what? It's nothing new. It's not due to recent political developments or world crises. This godless, lawless world has been around for thousands of years – and so has wickedness. Ever since Satan tempted Eve in the Garden of Eden. Truly, there [...Read More]
A Blended Psalm of Praise
A Psalm. For giving grateful praise. Praise the Lord, my soul; all my inmost being, praise his holy name. Praise the Lord, my soul, and forget not all his benefits. Great is the Lord, and greatly to be praised, and his greatness is unsearchable. SHOUT for JOY to the Lord, all the earth. Worship the Lord with gladness, come before him with joyful songs. Know that the Lord, HE IS GOD! It is he who made us, and we are his; we are his people, and the sheep of his pasture. Enter his gates with thanksgiving, and his courts with praise! Give thanks to him; bless his name! For the LORD IS GOOD; his steadfast love endures forever, and his faithfulness continues [...Read More]


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This is a great smart sprinkler controller that will water your lawn using the NOAA weather forecast. This system is able to wisely service 16 stations, saving water and your money.
16 (12-station model is available)
What is a Smart Sprinkler
We can see groomed lawns not just in the parks or at the stadiums, but near buildings like hotels, banks, shops, filling stations, and country houses. Many of us like spending time in the backyard, drinking sprightly beverages and enjoying the view of the lawn. But then, what should we do to keep our lawns beautiful and alive, and prevent their drying? Basically, there are two common ways to water the lawn - manual and using irrigation controllers (smart sprinklers). Manual watering requires fussing with buckets and hoses, which takes a lot of time and effort. Contrarily, irrigation controllers make watering easy and automatic. They pick the data from ground humidity sensors and/or weather forecast centers and provide the needed amount of water not to dry out and not to overwater the lawn. Most sophisticated sprinklers can be controlled via smartphone from any corner of the world and may cover up to 48 watering stations. We have considered smart sprinklers and their key features to help you choose the right model.
You should also mow your lawn to keep it nice. Our best lawn mowers review will help you pick a proper lawn mower for your needs. Also, you might need a good leaf blower for keeping your pathways clean.
What Features to Compare
Performance is the first thing that deserves your attention when you choose an irrigation controller. In particular, the number of stations is the key feature of any controller. Thus, the number of stations is normally equal to the number of sprinklers controlled. An irrigation controller may maintain up to 48 stations, so you should consider the area of your lawn for accurate irrigation. Some models may be connected to additional modules to increase the number of serviced areas and enhance the sprinkling capabilities. The number of programs is the next important parameter to consider. The programs may be automatic or manual and provide accurate, correct irrigation. Some controllers allow you to set the programs yourself according to your lawn conditions. The performance of a controller may be affected by operating temperature range. Thus, if the summer in your region is hot, you should pick the controller capable of working at high temperatures. Otherwise, the controller will fail and your lawn will not be watered properly.
You should pay attention to control that allows you to comfortably set the controller for managing the stations effectively. The irrigation controllers commonly have electronic or mechanical controls. The most sophisticated models are equipped with touchscreen displays that provide full control over irrigation process. Yet, this is not the whole story. Most of the irrigation controllers can be controlled wirelessly via smartphones based on Android or iOS. The control is performed through the built-in Wi-Fi modules that synchronize with smartphones through the web. You just need to download the official app to control the irrigation from anywhere around the world. This way, you can set the parameters of a controller as well as program the irrigation modes while being on vacation or business trip.
The irrigation controllers usually come with additional features. For a start, decide on the type of a controller as controllers are available for indoor or outdoor use. Indoor controllers are convenient in use as you can activate irrigation, staying in your house. The downside of these models is a complex mounting and the need to pull the wiring into your house. Outdoor irrigation controllers are usually mounted outside the buildings, near lawns. They are waterproof and feature lockable doors that forbid unauthorized use. However, it should be noted that the indoor models may be also mounted outdoors; they just need special outdoor housings for protection. Other features of an irrigation controller may include automatic diagnostics of all system units and connection of additional modules to expand the irrigation capabilities. Some irrigation controllers may serve as such modules and can be integrated into home automation systems. Hence, you can control not just your house but also the lawn watering with a single system. Furthermore, you can equip an irrigation controller with moisture and weather sensors to let the system determine the right amount of water and the duration of sprinkling.
RainMachine HD-16
Your Carte Blanche
Today we will review the RainMachine controller made by Green Electronics. It maintains up to 16 watering stations on your lawn and can operate them all either simultaneously, sequentially, or in parallel, thus enhancing the functionality and providing you with a freedom of choice. If your lawn is a big one, you may purchase a model for 12 stations offered by the same manufacturer. In this case, we recommend calculating in advance how many watering zones should be covered by a smart sprinkler and buy a controller with the excessive capacity. We liked the option of separate programming for each station depending on the lawn location and conditions. There is an unlimited quantity of available programs for every watering station, i.e. you can set parameters taking into account the specifics of each zone. We have noticed that the sprinkler works in a wide range of temperatures. Whether it is a cold winter or a hot summer this controller will continue performing perfectly! We believe that the major advantage of the RainMachine sprinkler is its touchscreen display, which is almost the size of a tablet. The display does not only reflect all relevant information but also allows controlling all watering stations. It is very convenient. That is why currently sensor displays are used worldwide to control various devices. The built-in Wi-Fi adapter provides you with a comprehensive control over the smart sprinkler from any PC using a browser or a smartphone if you downloaded a special application. You can find a lid under the screen covering additional mechanical controls. If you feel unsatisfied with pre-programmed functions, you might adjust or fine-tune any program you like.
Lawn Watered, Cash Saved
Once again, why is this controller called "a smart sprinkler"? The answer lies in its specific features. And the main feature is the Internet access. You got it right, the data downloaded from the Internet helps organize a proper irrigation of the lawn, i.e. based on this information the controller will adjust the sprinkling schedule. The source of weather forecasts is the NOAA service. Those who have not heard about this service - we assure you that it is considered to be the most trustworthy weather forecast in the world. Nevertheless, we have found out that to ensure the ultimate accuracy of forecasting the controller collects information from other sources too. We would also like to draw your attention to the Precision Forecast function that determines the exact location allowing the controller to forecast the weather even more accurately. Having studied the manual we discovered that your location data is stored in the controller itself instead of Internet servers. It is an evidence of the manufacturer's care about safety and confidentiality of your information. You also should not worry about an excessive water spending. The RainMachine is EPA WaterSense certified for the efficient and effective saving of the water resources. This means the smart controller saves you money every time it sprinkles over your lawn. Our overall conclusion is that this model would be a perfect choice for the people wishing to have the full discretion and control over every inch of their lawn. This controller takes care of your property as you would do it yourself.
More Products to Consider
Consider a Granite Rock Sprinkler System Valve Cover Boxfor additional protection of the irrigation system.
To achieve more diverse watering options, you can purchase a Brass Impact Sprinkler and increase sprinkling radius from 20 to 41 feet long.
Additional Info
SkyDrop SDCRW1.0_FFP
Move the Jog
Let's take a careful look at the SkyDrop controller for an automatic irrigation system. This model maintains up to 8 watering stations, though the 16-station version for larger lawns is also available. It is worth mentioning that the controller allows programming each watering station separately. Why do we single out this feature? Let's assume that there are trees or other barriers to sun rays, in this case, some part of a lawn should be watered seldom as it is less affected by the direct sunlight and the soil is less dry there. Additionally, you may develop your own programs to fine-tune irrigation conditions. This function will be appreciated by the most scrupulous landlords. Initially, this model was designed for indoor installation, but you can buy a protection housing to place it outdoors, the range of working temperatures of this smart controller allows that. By the way, the upper limit of this range allows using the controller in tropical countries or under the direct exposure to the sun. We also liked a simple and easy-to-understand interface with extra comfortable jog dial assuring a straightforward control over the irrigation process. In the XXI century, a smartphone became an integral part of our daily routine. Why do we remind you this? We would like to inform you about a simple and convenient application for this smart sprinkler, developed by the manufacturer. You can adjust all settings using your smartphone and not get back to the controller to do every change. The device will notify you with a message about any event related to the irrigation system. All these options are featured by the Skydrop app for Android and iOS that you can download following the links below.
Seeks and Finds
Concise tests of the controller brought to our attention the function of Auto Zone Detection. The first controller start initiates testing of all its units for their performance. If a failure is found the Fault Detection function displays its cause and potential solutions. This self-test is one more reason to call this controller "a smart device". In the course of our work we became curious, if this watering system is cost-saving at all? And we can reassure you that it is a real miracle. The pre-set Water Restrictions feature tracks the water consumption regulations in your community and adjusts the irrigation schedule to maintain the necessary soil humidity without exceeding the watering limits. If you care about the environment and your family budget you should consider buying this smart controller. One more plus is the Expansion Module feature of the smart sprinkler that allows adding an extra sprinkler module and expanding the watering zones any time. We believe this controller would perfectly satisfy owners of small lawns or the persons planning to expand their meadows. The option of expansion provides the possibility to add new watering zones constantly and rids you of the necessity to buy a more powerful sprinkler system in that case.
More Products to Consider
Consider buying a wireless lighting control On/Off switch, another smart gadget to keep in household and control lightning in the backyard from smartphone.
A motion activated sprinkler will let you keep different pests away from your home by sprinkling water without its excess waste.
Additional Info
Rachio 8ZULW-B
Smartphone. That's All You Need
This time, we review the Rachio smart sprinkler. This particular model supports 8 watering stations though you might buy a version maintaining 16 sprinklers. We have immediately distinguished its easy control and simple design. There are two optional operating modes of this controller: a fixed schedule for watering in set moments of time, and a flexible schedule for irrigation when needed. You may install additional rain and soil humidity sensors to adjust the watering controller's work precisely. By the way, you can install this device both indoors and outdoors, if you equip it with an outdoor protection casing. We would also like to mention that the manufacturer has made it possible to utilize the smart sprinkler under high and low temperatures, so it will work regardless of weather changes. Possibly, the main difference of this controller from its competitors is the absence of a display and controls. The users are divided on this issue. One group considers this to be not very convenient arguing about a potential lack of skills among users of the smartphone apps. Others opposed pointing at the available benefits as the absence of controls enables access to the smart sprinkler only to those who installed the application. Furthermore, the app itself proved to be foolproof and functional. The remote access, messaging, and information on a current status and water consumption are constantly accessible anytime and from any place. The links for downloading the applications are provided below separately for Android and iOS devices.
First Step to Smart Home
We were impressed particularly by one of the controller's features, namely the Eliminate Runoff function. It allows the smart sprinkler to schedule shorter irrigation cycles with the objective to increase the intensity of water absorption by the soil. This way the soil will not be overwatered and its upper layer will be protected from the erosion. Same as any other watering controller with the Wi-Fi connection this smart system receives weather forecasts from the Internet: it will not work shortly before the rain, while it is raining, and immediately afterward; also, no watering is performed when it is cold. We have also liked the Weather Intelligence™ function that monitors weather changes for a certain period of time. And we would like to reassure you that you should not worry about adjusting the sprinkling schedule. Your controller will do that for you. The Rachio controller is EPA WaterSense certified and SWAT tested. It helps save the water resources, decrease the environmental footprint, and reduce your expenses.
Finally, you may benefit if the controller is included into a "smart home" system. Considering versatility of its functions, this solution sounds reasonable. Moreover, this smart controller is compatible with accessories and home appliances made by Nexia, Crestron, Control4, Wink, and Nest.
More Products to Consider
Use a Wired rain sensor to collect precise data on humidity and atmospheric pressure and come up with the right weather forecast each time when needed.
A Spray Head Pull-Up tool will come in handy if you need to lift up a sprinkling head.
Additional Info
Orbit 57950
Three in One
It's high time to get acquainted with the Orbit smart sprinkler. It maintains up to 12 irrigation zones though if your lawn is not that large you might stick to the model servicing 6 zones only, offered by the same manufacturer. Observing the test performed on this controller, we enjoyed an innovative approach to its software package. There are 3 options for different irrigation plans: for example, program A monitors your lawn, program B keeps your flowerbeds wet, and program C waters your garden. This sprinkler system also allows selecting from two alternative control methods: a mechanical control with buttons on the control panel and a remote control from a smart device. It should be mentioned that the working temperatures of the controller range from 32°F to 158°F. We would recommend its use exclusively in a warm climate.
Water Resistancy
We would like to put particular emphasis on the B-hyve technology developed by the manufacturer. It allows you to easily set a wide range of functions. In case you are confused with this technology the package includes a detailed training video on the set-up process. One more feature we particularly liked is a possibility to label individual irrigation zones and even to assign photos to them. Every time you select a watering zone you can see which plants are sprinkled at the moment. The smart sprinkler collects information from various Internet sites to make the watering process as precise and efficient as possible. It is also necessary to mention that you have a free choice to install the Orbit system indoors or outdoors right out-of-the-box. It is equipped with a weatherproof casing resistant to the moisture and can be mounted nearby the lawn. So, you do not need to enter your house to switch the sprinkler. The swing panel lock prevents an unauthorized access to the controller.
In conclusion, it should be said that this device is most appropriate in case you are not able to install the watering controller indoors. An additional advantage is a possibility to connect controller directly to the power switchboard by-passing the wall outlets. You only need to take off the plug.
Some Facts About Orbit
The Orbit Company is the world leader in manufacturing and selling irrigation systems, misting systems and home appliances. It is present in 40 countries from 5 continents. Its production capacities encompass national and international R&D, design works, and cutting-edge technologies for casting and injection molding.
The company management has a clear vision of the production targets striving to exceed client expectations and ensure the ultimate quality of its products at the most favorable price. Orbit does not stop to progress and move forward, they constantly seek perfection and introduce new goods that help their clients save time, water, and money.
The company's objective is to deliver products that would satisfy the pickiest clients and guarantee functionality, long useful life and simplicity.
Useful Links
A rain and freeze sensor can sense rain and frosts and inform you of a weather change in time.
You may want to use 1- or 2-horsepower pump start telay to automatically start the pump while watering the lawn.
Additional Info
Hunter Sprinkler PCC1200I
Turn On and Forget
The Hunter Sprinkler PCC1200I controller can easily handle 12 watering stations on your large lawn. Is your garden not so big? It is not a problem, you may acquire the 6-station model! But let's start from the very beginning. The system features three pre-programmed options to maintain three different irrigation plans: a lawn, a garden, and a flowerbed. It is a common approach. The controller allows setting specific days of the week, odd and even dates, target zones for watering and no watering days. We would like to draw your attention to the fact that the system works under temperatures in the range of 0°F through 140°F making it appropriate for use in the mild climate states. Although this controller is called "a smart sprinkler" it can be managed by anyone. It features a classical control technology based on the mechanical switches and buttons. There are no touchscreen displays and Wi-Fi modems, and you do not have to install any smartphone apps or to set Internet connections. Just turn it on and select a program, and the smart sprinkler will start working. Actually, there is a monochrome display on the front panel reflecting all relevant information.
Lawn and Light Control
Speaking about peculiarities we should mention a possibility to install this controller both inside the house and outside it, since it has a waterproof body. The system can be extended by adding the Solar Sync® set. The set includes three sensors for rain, low temperatures, and total evaporation rate (ET). These sensors monitor the local weather conditions, the intensity of water consumption by plants and the soil, and can stop the watering in case it rains or the ground freezes. We will provide a link to this sensor system below. It may help you save water and your money. Another pleasant feature for us to discover was controller's ability to adjust surrounding illumination. Its transformers are controlled by the PXSync interface allowing you to manage all functions from a single point.
After all the research done, we asked ourselves: "Who would appreciate this device?" And our conclusion was that it might be the best choice for champions of simple tools without any frills like extra electronics or the Internet access, for the people learning about the weather from TV and radio broadcasts, and for individuals demanding only the reliability and simplicity of their house appliances.
Useful Links
Solar Sync Kit is an additional weather forecast module with built-in rain and frost sensors for switching the sprinkler system off when necessary.
ROAM-KIT Controller Remote Complete Kit is an extremely powerful remote for controlling this smart sprinkler at a distance of up to 1,000 feet.
Additional Info
1. Cabe Atwell Smart Sprinkler Systems Save Water and Save Houses, Sensors. December 2, 2015.
2. William Finucane Create a Smart Sprinkler System to Water Your Garden When the Soil Dries Up, Mad Science.
3. Klint Finley Smart Sprinkler Checks the Weather to Avoid Wasting Water, WIRED. November 24, 2015.
4. Tips for Selecting an Irrigation Controller, Chandler.
5. Selecting a Smart Irrigation Controller, University of California. June 29, 2010.
6. Irrigation Controller, Wikipedia. May 29, 2015.
7. Irrigation Controllers for the Homeowner, Department of Water Resources.
8. Smart Irrigation Controllers, SRP.


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good natured living
About Us
Our products
activated organic cereals
activated organic crackers
activated organic biscuits
activated organic pizza bases
Contact Us
Payment & Postage
Our services
catering... cooking
Good Natured Living Services
We live in a beautiful place alive with Nature, especially birds and we would like to share this with others, we can offer these services to you from our home so that you can also enjoy this place.
Please contact us to arrange cooking classes or treatments at our home.
Book in for a relaxing treatment at our beautiful home in the Sunshine Coast Hinterland. Enjoy an organic tea or juice with the birds afterwards.
Mark combines remedial massage with trigger point therapy and pranic healing (energetic) and Tibetan pulsing. We can also help with dietary changes, detoxing and emotional issues.
We love to cook and Sascha has had many years of experience catering and cooking for different events & occasions of various sizes. Sascha loves specialising in menus which cater for different dietary needs including gluten free, grain free, vegan, vegetarian, paleo and raw living food.
It's a family affair at Good Natured Living and we also have a very experienced private bartender available for your event who can offer non-alcoholic alternatives as well, juices, superfood "mocktails" & elixirs.
ease enquire for more information and to discuss your event.
Sascha would like to pass on her knowledge and passion for healthy, organic food. We can offer cooking classes for individuals, families or groups of up to 10 people from our home kitchen in a beautiful peaceful environment 20 minutes from Maleny.
Would you like to learn more about activated organic food, vegan food, vegetarian, superfoods and supplements, fermented foods, herbs and vegetables or paleo. Sascha loves real, natural and delicious food drawing from many years of personal research, dietary changes and overseas travel inspiration. Sascha is also passionate about bringing the family together around the dining table for everyday meals and special occasions. For her the kitchen is the physical heart of the home and she loves to involve all the family in her culinary adventures.
Please contact Sascha and Mark for more information:
0429 420 046
Thank you
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activated organic cereals
activated organic crackers
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text: Amsterdam is the city of activity. It's bursting with people speaking all languages and moving with cars, boats, ferries every which way! The city is extremely beautiful by night! Share this postFacebookTwitterGoogle+PinterestLinkedIn About the author Abby Savage More StoriesWashington DC SpringtimeJune 27, 2016NYC circa 2009June 27, 2016New York HarborJune 27, 2016Paris-Eiffel Tower, Notre Dame, Sacre CoeurJune 21, 2016Koblenz and Heidelberg, GermanyJune 21, 2016Chicago, My Kind of Town!June 5, 2016


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Do I need to bring a laptop?
It's a good idea if you have one. We'll have wifi and places to charge up.
Do I have to register for individual sessions?
No. We say you vote with your feet. If you like the session you're attending, stick around. If you find that it's not working for you, politely leave and go to another one. This might be the most casual conference you've been to!
What's for lunch?
On Friday, Qdoba. On Saturday, City BBQ. Sunday, we're not sure yet! We'll have vegetarian options all days, too.
Can I bring my spouse to the after party?
Of course! We're covering appetizers for everyone, but you're on your own for drinks and food. It's at Miller's Ale House on near the intersection of Olentangy and 3rd.
Can I register at the door?
Yes, but we can't guarantee you'll get a tshirt and your namebadge will be handwritten. HANDWRITTEN.


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His own boss: a Cossack commander in eastern Ukraine
"Colonel" Pavel Dremov salutes the flag of the Great Army of the Don Cossacks. And he answers to no one.
Cutting a striking figure in his astrakan cap, the stocky 38-year-old shows AFP into his office, a police station commandeered by his men, a stone's throw from a bronze statue of Alexei Stakhanov -- a socialist hero of Ukraine's eastern rustbelt.
Sandbags protect the windows and slabs of reinforced concrete shore up the main wall, while inside women in camouflage trousers stir pots of borscht.
The town of Stakhanov is on Dremov's section of the frontline between pro-Russian separatists and Ukrainian soldiers -- his private fiefdom that amounts to a rebellion within a rebellion.
"If I had to obey someone it would be my ataman (chief), Nikolai Ivanovich Kozitsyn," Dremov said, referring to the head of the Cossacks of the Don River, an eclectic mainly eastern Slavic group with military and cultural traditions dating back to the 16th-century Russian Tsar Ivan the Terrible.
"He is my direct superior. We Cossacks are fighting for a cause, a faith, an ideal: the New Russia."
Russian President Vladimir Putin set off alarm bells when he used the phrase "New Russia" when referring to eastern Ukraine. In Russian, "Novorossiya" is an archaic term for an area that was controlled by Russia under the tsars.
Though Dremov is adamantly his own boss, it is clear where his sympathies lie.
"Of course I am collaborating with the forces of the Donetsk and Lugansk 'people's republics'" proclaimed in April by the pro-Russian separatists.
"I am waiting for the Lugansk defence minister. He's a close friend," adds Dremov, whose green uniform without insignia is similar to that worn by Russian soldiers who entered Crimea in March before Moscow annexed the peninsula.
He even says he organised and took part in an assault on the Ukrainian secret service building in Lugansk, some 50 kilometres (30 miles) east of Stakhanov.
Then, lowering his voice, Dremov says: "I'm going to tell you a secret. I have 1,200 fighters under my command, but only one broken-down tank and a troop transport. That's all."
AFP has no way of verifying the claims of the "colonel", who gets around in a Japanese SUV painted in camouflage colours, shuttling between Stakhanov and the nearby town of Pervomaisk.
On September 12, when observers of the European security body OSCE met Dremov, he told them he had 700 Cossacks and three 122 mm Howitzers.
Rebel authorities in Lugansk confirmed to the observers that Dremov did not receive orders from them.
Dremov says the shaky ceasefire accord reached in Minsk between Kiev and Lugansk and Donetsk on September 5 is a sham.
"This agreement in Minsk only calls for the withdrawal of weapons of more than 100 mm calibre," he says.
"Do you think that an 82 mm mortar doesn't kill? The accord says nothing about tanks. Last night, the Ukrainians hid nine of them three kilometres (two miles) from here. And they fired an 82 mm mortar on civilian buildings. That's not a violation?"
"What I think is that the Ukrainians have understood that we were using much better artillery than theirs, and they found this way to (reduce our capability). This accord is a trap. I don't recognise it."
- Secret agent? -
In the Ukrainian press and on Internet sites, the "colonel" is accused of being Russian, a secret agent, an officer sent by Moscow like the "mercenaries" or soldiers sent en masse into Ukraine -- which Moscow denies.
"In fact, I was born here in Stakhanov," Dremov says. "My passport's in the car, my assistant can go get it for you if you want."
Dremov, a mason by trade, says he served in the Ukrainian army from 1994 to 1996 near Odessa, showing a tattoo on his chest giving his blood type in Ukrainian as proof.
But he also admits fighting alongside pro-Russian rebels during the 1992 conflict in Transdniestr, a strip of land in eastern Moldova with a population of half a million that won independence with Russian help but never gained international recognition.
"They say I have Russian volunteers. Yes, it's true. Of my 1,200 men there are 13 of them, including two doctors," he smiles. "I never asked to become a commander. When all this started I was a mason working in Moscow.
"What I want is for people to be able to express themselves freely. Me, what I want is the independence of New Russia. But the people will decide," he said.
The separatists have called elections for November 2.
"And when that is over," Dremov said, "I have promised to go back to being a mason, to live peaceably on my land. To rebuild everything they've destroyed."


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Author Archives: chrisk
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text: 000 WUUS53 KICT 220230 SVRICT KSC015-017-073-220315- /O.NEW.KICT.SV.W.0244.170822T0230Z-170822T0315Z/ BULLETIN - IMMEDIATE BROADCAST REQUESTED Severe Thunderstorm Warning National Weather Service Wichita KS 930 PM CDT MON AUG 21 2017 The National Weather Service in Wichita has issued a * Severe Thunderstorm Warning for... Southeastern Chase County in east central Kansas... Northeastern Butler County in south central Kansas... Northwestern Greenwood County in southeastern Kansas... * Until 1015 PM CDT * At 930 PM CDT, a severe thunderstorm was located near El Dorado, moving northeast at 40 mph. HAZARD...60 mph wind gusts and penny size hail. SOURCE...Radar indicated. IMPACT...Expect damage to roofs, siding, and trees. * Locations impacted include... El Dorado, Cassoday, Matfield Green, El Dorado Lake and El Dorado State Park. This includes Interstate 35 between Mile Markers 73 and 115. PRECAUTIONARY/PREPAREDNESS ACTIONS... For your protection move to an interior room on the lowest floor of a building. && LAT...LON 3777 9686 3798 9692 3829 9636 3817 9636 3817 9635 3795 9635 TIME...MOT...LOC 0230Z 240DEG 34KT 3791 9685 HAIL...0.75IN WIND...60MPH $$ DARMOFAL


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Ok this is part embarrassing and part funny, but you will still feel for this poor girl who was being interviewed on live tv and needed to pee. She tried to hold it, but failed and wet her pants. Shame man. She did handle it rather well though given that she was being interviewed about witnessing a shooting.
Here's the full video, but you skip to the 1 min mark where she starts squirming.
That bit when the reporter asked her what the victim said and she replied 'he said help, me help…i got to pee'.


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- Trending Now: More Trending:
- Svengali syzygy hegemon repugnant white nationalist, white supremacist
Definition of circumincession
: the theological doctrine of the reciprocal existence in each other of the three persons of the Trinity
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Origin and Etymology of circumincession
Medieval Latin circumincession-, circumincessio (from Latin circum- + Medieval Latin incessio, from Latin incessus, past participle of incedere to go along, from in- + cedere to go), translation of Middle Greek perichōrēsis, from Greek perichōrein to go around, rotate, from peri- + chōrein to go, withdraw (akin to chēros left, bereaved)
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text: A series of 15 collectible figurines shaped after popular knights of Scotshold such as Christian, head of the Royal Knights, or Aleslot the Bowlord. Retrieved from "http://fortune-summoners.wikia.com/wiki/Knight_Figurine?oldid=4394" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Items Add category Cancel Save


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Prospective evaluation of the leukocyte adherence inhibition (lai) test in breast cancer using a panel of extracts from known and unknown primary tumors
Version of Record online: 17 JUL 2006
DOI: 10.1002/ijc.2910290306
Copyright © 1982 Wiley-Liss, Inc., A Wiley Company
International Journal of Cancer
Volume 29, Issue 3, pages 261–264, 15 March 1982
Additional Information
How to Cite
Fritze, D., Fedra, G. and Kaufmann, M. (1982), Prospective evaluation of the leukocyte adherence inhibition (lai) test in breast cancer using a panel of extracts from known and unknown primary tumors. Int. J. Cancer, 29: 261–264. doi: 10.1002/ijc.2910290306
Publication History
- Issue online: 17 JUL 2006
- Version of Record online: 17 JUL 2006
- Manuscript Revised: 12 JAN 1982
- Manuscript Received: 22 OCT 1981
Funded by
- Deutsche For-schungsgemeinschaft. Grant Number: SFB 136
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Tips to Manage Stress in Your Jobs
The common symptoms of stress are the following :
- Negativity
- Loss of interest and focus
- Low self-esteem
- Boredom
- Depression
- Irritability
- Physical problems such as headaches and stomachaches
If you feel like you are experiencing these symptoms then better do something about it because stress can lead to your ruin and nobody can help you more but yourself. The demands of your job might increase from time to time but you have to realize that your job is not the only thing that defines you. Identify where your stress is coming from and deal with it without having to compromise the important things in your life.
Here are the 5 important steps on how to manage stress in your jobs :
- Change your outlook in life – keep in mind that your job is not permanent and as much as you would like to keep it, there are other important things in your life that you need to focus more in the years to come such as your family, your health and your hobbies. You always have an option to leave your job when it becomes too stressful to handle but make it as the last resort.
- Take a deep breath – if you feel that the stress you are feeling is already starting to build up, you can breathe in and breathe out or take a break just to clear your head. Go to the park and just watch the good times taking place among strangers. Do something that will relax you even just for a few minutes.
- Talk about it – go to your closest colleague in the office or someone who shares the same sentiments and just talk it out. Be generous and share it to someone you trust, don't just keep it to yourself because it will make you crazy.
- Laugh it out – go to YouTube and watch the funniest videos you can find and laugh. They say that laughter is the best medicine so use it to heal you even just for a day.
- Stay Positive – keep a positive mind no matter how hard your situation is because negativity won't bring you any good in battling stress.
These easy steps will help you during your tough times at work and this is a great strategy if you are working on your career management skills. Understanding your situation and the result of these steps will make you more focused on your job and can open more career opportunities for you in the future.


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Competitiveness and growth in EMU: The role of the external sector in the adjustment of the Spanish economy
This paper assesses the relevance of the competitiveness argument in the debate on the current situation of the Spanish economy. We estimate error-correction models for exports and imports. Econometric analyses suggest that external demand and domestic activity are the more important determinants of net exports in the long run. However, where competitiveness is concerned, its impact on export growth in the short run appears to be higher than in the longer term. Moreover, during major downturns, the recovery of the Spanish economy has systematically been led by exports, which, in turn, has been associated with strong and rapid competitiveness gains, brought about by competitive devaluations. Since the exchange-rate instrument is not available anymore at national level, there is a case to assess the respective role of fiscal and structural policies in underpinning the recovery of the Spanish economy at the current juncture. The paper concludes that the room for manoeuvre for fiscal-policy stabilisation is currently very limited. In contrast, reducing the weight of distortionary taxes and/or increasing the weight of productive expenditures in a budget-neutral way would have positive impacts on potential growth. Boosting net-export growth crucially depends on the implementation of a comprehensive reform programme, within the framework of a broad social agreement, aiming at recovering competitiveness throughout enhancing competition, improving the functioning of labour and product markets, including the housing rental market, increasing physical, human and knowledge capital accumulation, promoting wage moderation and avoiding price-wage spirals. A pro-active role of the Spanish government in the social dialogue would provide guidance in the process and promote the agreement
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|44 pages
|Date of creation:
|Jan 2009
|Contact details of provider:
| Postal: Inter-institutional relations and communication Unit, B-1049 Brussels
Fax: +32 2 298.08.23
Web page: https://ec.europa.eu/info/departments/economic-and-financial-affairs_en
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The indie rock band released 'Three Rings', the first single from the long-awaited new album, upon the world at the start of May. It wasn't totally unexpected. They've been keeping their fans in suspense for a while now by updating their instagram account with short teasers.
The wonderful vocal harmonies of Ed Droste and Daniel Rossen leave none unmoved and also approach perfection live.
Beach House, Local Natives, Jagwar Ma, Tame Impala, Animal Collective, St. Vincent, Deerhunter, Unknown Mortal Orchestra
'Grizzly Bear klinkt rijker dan ooit zonder hun toegankelijkheid te verliezen. Petje af!' –Dansende Beren
Grizzly Bear already played at the Feeërieën in 2006.
Would you like to make your concert evening in the AB into the perfect night out? First something nice to eat, go have a drink before or after the concert? Click here
Our concerts usually end at 10:30 pm. That way you can still easily get home on public transport.
With the Event Pass code at the top of your ticket, you can make use of tram, bus and metro in Brussels for your route to and from the AB on the day of the concert. More
Surprise someone with a concert. Order the AB concert voucher online!
Quickly fill in the list of your Favourite Artists. That way you will be first to be informed when they plan to visit AB.Continue


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World Asset Management Inc Has $6.70 Million Stake in BP plc (BP)
Finally, Canaccord Genuity restated a "buy" rating and set a GBX 525 ($6.84) price objective on shares of BP plc in a report on Wednesday, May 3rd. Credit Suisse maintained the stock with "Neutral" rating in Friday, August 14 report. BP p.l.c. shares had a trading volume of 746K by the end of trading on Wednesday. If you are viewing this piece on another site, it was illegally copied and republished in violation of United States and global copyright & trademark law.


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The Century
no rating
Restaurants - Pyrmont, NSW
The Century - Pyrmont offers Chinese, Seafood, Vegetarian, Yum Cha Chic Modern Chinese restaurant with live seafood tanks, specialising in lobster, abalone and crab.
THE CENTURY captures the essence of Golden Century, which has been a popular Sydney restaurant for 23 years, serving Golden Century's favourite classics and signature dishes.
Classic taste: The Century captures the essence of Golden Century, which has been a popular Sydney institution and the group's Chinatown flagship restaurant, by serving everyone's favourite classics and signature dishes.
New look: Be ready to be wowed the moment you step in! Admire the 15 double glazed aquarium tanks featuring live seafood at our entrance; Sniff the American oak used sparingly across the bar and dining areas as you appreciate our matching wine; Feel our feature wall handmade with 82,320 chopsticks throughout the restaurant and twinkle with the hundreds of tea cup lights, our stylish backdrop over hanging at our semi private dining areas.
Behind all these is a new interior concept brought to you by Hong Kong based Paring Onion Design, paying tribute to Chinese culinary culture with a refreshing and modern twist. In September 2013, The Century was announced the proud Winner for Best Restaurant Design (Australia & Pacific) by the International Restaurant & Bar Design Awards in London.
Wine& Dine: Our cellar is home to an extensive list of value and premium wines from the likes of Australian, France, Italy, Germany, Portugal, USA, Japan and China. Old and new world, young and mature, value and premium, our award winning wine list will bring your dining experience to another level.


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Permalinks, changing the htaccess
I currently have a standard htaccess file in domain.com/press that looks like this:
<IfModule mod_rewrite.c>
RewriteEngine On
RewriteBase /press/
RewriteCond %{REQUEST_FILENAME} !-f
RewriteCond %{REQUEST_FILENAME} !-d
RewriteRule . /press/index.php [L]
However, I want to add this:
RewriteCond %{HTTP_HOST} !^www.domian.com$ [NC]
RewriteRule ^(.*)$ http://www.domian.com/$1 [L,R=301]
The second rewrite comes from root (RewriteBase / ). I'm trying to figure out how to modify the wordpress rewrite so I can make the base at root level.
- The topic 'Permalinks, changing the htaccess' is closed to new replies.


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Whether you like to do mobile repairing as a hobby, side business or whether it's your professional business, you all should be awa...
Below I've shared the list of the tools that you must have -
1. Multimeter - It is used to check PCB track and it's electronic components.
2. Case/Screen Opener - Used to easily open any mobile case or to open it's screen.
3. Brush - Used to clean PCB
4. Thinner - Used to clean PCB
5. Blade Cutter - It is used to cut and remove any lamination which may be on the phone.
6. Battery Booster - Used to boost voltage of a mobile battery.
7. Magnifying Lamp - Used to get a magnified view of PCB and its components.
8. Screwdriver Tool Kit - To disassemble and assemble a mobile or tablet.
9. Tweezers - Used to hold a wire and components.
10. Soldering Iron & Wire - Used to solder.
11. Flux - Used while soldering.
12. Jumper Wire - Just to connect one point to another in PCB.
13. Solder Paste - Used when doing HRC (Heat Reball Change) on an IC (Integrated Circuit).
14. PCB Stand - Used to hold a PCB.
16. BGA Kit - Used to reball and repair (HRC) a ball type IC.
17. Reti Or Sponge - Used to clean tip of Soldering Iron.
18. SMD Machine - Used to remove and attach an electronic components on a PCB.
19. DC Power Supply - Used in supplying a DC Electricity.


text:
iPromise Not to Tell is the 12th episode of the first season of iCarly.
A ridiculous technicality costs Carly her streak of straight A's. The next day Sam is being punished, she manages to fix Carly's grades with the school computers(and for some reason,Freddie's), and makes her promise not to tell anybody. because Sam's scheme involves Freddie, Carly decided to tell only him about it, and Carly can't decide whether the guilt over Sam's amateur hacking or Freddie's sophisitcated effort to undo the fix will knaw away at her conscience first.


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Cat Veterinary
Have Cat Questions? Ask a Cat Vet Online.
Hi there and thank you for your question. My name is ***** ***** I am a licensed, internship-trained veterinarian with 10 years experience in general medicine and emergency and critical care medicine and I would be happy to assist you. Please be patient as there may be slight delays in responses as I research your pet's issue.
Most frequently, especially in older females, this is a medical problem. There could be crystals in the urine causing bladder irritation, or a chronic urinary tract infection, or a bladder stone. The first step here is to see your veterinarian for a urinalysis and potentially a bladder ultrasound. If a problem is identified, your veterinarian can treat it, and the problem should resolve. If no problem is identified, then your veterinarian can suggest behavior modification medications.
I hope that I have answered your question thoroughly. If you still have questions, please reply. If you feel that I have provided anything less than excellent service, please reply to me first before rating my service and let me know how I can better assist you. Thank you for your question; it has been a pleasure being able to provide assistance. Best of luck.
Cats and dogs do not have heart attacks. She would be a little bit stressed, but she would recover from it. you asked if there is a guarantee that either of your cats could be helped… Of course not. But there certainly is no way to help them without finding out what is wrong. I charge $20 for a brief bladder ultrasound, and $350 sounds like a lot. There really aren't many techniques you can try at home without knowing whether this is behavioral or medical. You could try buying a Feliway diffuser on Amazon.com... You plug it in and it releases a pheromone that calms and soothes cats. You can help with behavioral or stress urination issues. If you do not want to take the Torti to the veterinarian, that is what I would try. Best of luck!
I would do a urinalysis on the torti before an ultrasound. A brief bladder ultrasound to check for stones or masses in the bladder takes about two minutes. That's why I don't charge much for it. But a full abdominal ultrasound is only $140 at my clinic here in Michigan.
Yeah, when I worked at Fresh Pond Animal Hospital in Cambridge the prices were much higher than here in Michigan. I would just call around and price shop.


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Search Collections
I Finally got Him
ca. 1910s W. H. Martin Born: 1865 Died: 1940 gelatin silver print 3 3/8 x 5 3/8 in. Smithsonian American Art Museum Gift of Charles Isaacs and Carol Nigro 2002.79.14 Not currently on view
Figure male
Object - weapon - axe
Recreation - sport and play - fishing
photography - photoprint
gelatin silver
About W. H. Martin
Born: 1865 Died: 1940
More works in the collection by
W. H. Martin
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No. 5 Oak Lawn was served notice that repeating as state volleyball champions is going to be anything but easy Thursday, dropping a SICA North match 7-15, 15-13, 15-8 to Sandburg.
And it was Wil Koh's serving that was the difference. The senior tallied 17 service points, including four straight to rally Sandburg (5-1, 2-) from a 13-11 Game 2 deficit.


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Register MFT mare, 6 yrs old. Red Dun. Great trail horse but is herd bound. Needs experience rider. She will be a great horse but I don't have time…$2,500
Gaston, a handsome, well built Belgian draft gelding with a most amazing mane. He stands at a stout 16 3 1/2 hh and is only 12 years old. Gaston is…$1,850
Degas is a handsome, all black 12 year old Percheron gelding. He rides and drives. He stands at a stout 15.2 hh. Before he arrived to us, Degas had all…$1,500
For your consideration, 9 year old gelding. Stands roughly 14.2 hands. Perfect disposition. Trail rides anywhere. Has no vices. Loads, leads, lunges…$4,000
Liza is a grade 3 year old appendix bred filly. She stands approx. 14.3 She was green broke last fall before coming to me and due to way to much going…$800
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After several years under the direction of a more experienced rider, Rowdy is ready to make his debut to the perfect home to the perfect rider. He is a…$2,000


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Board Members & Volunteers Come to our Board meetings -- 7:00 pm every third Monday of the month -- check the Calendar for the location.
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Why are we looking for new Board members?
Eastern Little League must maintain a strong Board of Directors. We are
looking for Board Members that are interested in making decisions that further
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Each year, new members are needed to replace the "aging out" Board Members
(who take years of experience with them). We have an ongoing need to recruit
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from these divisions will be the future Leaders of the Eastern Little League.
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Member to fulfill 100% of their assigned duties and responsibilities. Doing so will
prevent mistakes, problems and issues that to correct, drain energy, time and
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Member ?
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Conceived and designed the experiments: RK IS RPM GK. Performed the experiments: RK IS MD GP PC RPM VH. Analyzed the data: RK IS GP PC RPM BV GK. Contributed reagents/materials/analysis tools: RK MD GP RPM VH BV. Wrote the paper: RK RPM GK.
The authors have declared that no competing interests exist.
Essentially all knowledge about adult hippocampal neurogenesis in humans still comes from one seminal study by Eriksson et al. in 1998, although several others have provided suggestive findings. But only little information has been available in how far the situation in animal models would reflect the conditions in the adult and aging human brain. We therefore here mapped numerous features associated with adult neurogenesis in rodents in samples from human hippocampus across the entire lifespan. Such data would not offer proof of adult neurogenesis in humans, because it is based on the assumption that humans and rodents share marker expression patterns in adult neurogenesis. Nevertheless, together the data provide valuable information at least about the presence of markers, for which a link to adult neurogenesis might more reasonably be assumed than for others, in the adult human brain and their change with increasing age.
In rodents, doublecortin (DCX) is transiently expressed during adult neurogenesis and within the neurogenic niche of the dentate gyrus can serve as a valuable marker. We validated DCX as marker of granule cell development in fetal human tissue and used DCX expression as seed to examine the dentate gyrus for additional neurogenesis-associated features across the lifespan. We studied 54 individuals and detected DCX expression between birth and 100 years of age. Caveats for post-mortem analyses of human tissues apply but all samples were free of signs of ischemia and activated caspase-3. Fourteen markers related to adult hippocampal neurogenesis in rodents were assessed in DCX-positive cells. Total numbers of DCX expressing cells declined exponentially with increasing age, and co-expression of DCX with the other markers decreased. This argued against a non-specific re-appearance of immature markers in specimen from old brains. Early postnatally all 14 markers were co-expressed in DCX-positive cells. Until 30 to 40 years of age, for example, an overlap of DCX with Ki67, Mcm2, Sox2, Nestin, Prox1, PSA-NCAM, Calretinin, NeuN, and others was detected, and some key markers (Nestin, Sox2, Prox1) remained co-expressed into oldest age.
Our data suggest that in the adult human hippocampus neurogenesis-associated features that have been identified in rodents show patterns, as well as qualitative and quantitative age-related changes, that are similar to the course of adult hippocampal neurogenesis in rodents. Consequently, although further validation as well as the application of independent methodology (e.g. electron microscopy and cell culture work) is desirable, our data will help to devise the framework for specific research on cellular plasticity in the aging human hippocampus.
Adult hippocampal neurogenesis, i.e. the production of new granule cell neurons in the adult hippocampus, has captured the imagination of a wide audience and is beginning to influence hypotheses for clinical medicine. Adult neurogenesis is conserved in all mammalian species studied so far including non-human primates
Adult hippocampal neurogenesis in mice has been described in considerable detail, and distinct developmental stages have been identified
DCX is a brain-specific microtubule-associated protein whose exact function is not yet known. It appears to act as microtubule stabilizer in a way that is particularly pertinent to migration
DCX can serve as a marker for new neurons in the adult rodent hippocampus as long as the transient nature of its expression is taken into account. In addition, DCX is expressed elsewhere in the rodent (and human) brain, where no link to adult neurogenesis can be made
We also made a first attempt to gather semi-quantitative information on how expression of such markers might change across the lifespan as previously described for rodents
We performed western blot analyses of normal hippocampal tissue taken from a deceased child and resected hippocampus from a subject suffering from temporal lobe epilepsy with ammonshorn sclerosis (
Lane 1: Fetus of GW 20, elective abortion, cortical tissue; lane 2: child of age 3 years, healthy hippocampus; lane 3: adult of age 35 years, suffering from temporal lobe epilepsy (TLE), resected hippocampal tissue. The doublecortin antibody (sc-8066) reacted with proteins in the 45 kDa range, which corresponds to the MGrel. of DCX. The signal is strong in the fetal brain and much weaker in the juvenile and adult samples. The GAPDH signal at the 36 kDa position serves as MGrel and loading control.
We first examined DCX expression in the developing human DG. At GW11 we found indication of dense DCX expression on the level of both
A, B, The overlapping staining indicates the co-expression of DCX mRNA and protein. In cortical tissue (A), as well as in the ganglionic eminence (B), the mRNA is not restricted perinuclearly, but is also found in the transverse oriented processes of the round-shaped cells. C, Negative control of the
A, B, From birth to adulthood (A: 1 day, B: 28 years of age), hippocampal DCX labeling reveals a distinct staining pattern in the granule cell layer and hippocampus proper. C, In most granule cells (neonatal DG), the mRNA signal occurs outside the nucleus in a small cytoplasmic rim. D, Higher magnification of the DG area marked in (B). E, Application of a labelled sense cRNA probe to a serial section of (B). The absence of sense-probe labelling confirmed the specificity of the detection system for DCX-mRNA/cRNA antisense probe hybrids. Differential Interference Contrast. Scale bars A, B, 1 mm; C-E, 25 µm.
We focused on DCX expression in the DG. DCX-positive (DCX+) cells in the DG could be found in samples across the entire lifespan between 1 day and 100 years of age (exemplary in
Postnatal, numerous DCX+ neurite-bearing cells are scattered throughout the GCL (A). In the adulthood, DCX+ cells are gradually less differentiated in relation to growing age. Initially, some cells show a neuron-like morphology (B,D), whereas the majority of them appears increasingly more undifferentiated and strongly DCX-stained (B-F). In the juvenile mouse hippocampus a multitude of DCX+ cells delineate the GCL formation (G,I). Very old mice, however, exhibit only a few DCX expressing granule cells (H,K). DAB immunohistochemistry of DCX in paraffin embedded brain samples. Length of scale bars as indicated.
Increasing cellular differentiation (dendrites and axons) can be observed starting from the hilar polymorphic layer/subgranular layer (PML/SGL) throughout the GCL up to the inner molecular layer. However, undifferentiated round-shaped cells were always found throughout the entire GCL. This picture is a montage of DCX labeled granule cell and sorted increasingly maturated from left to right. Dendrites are directed towards the granule cell layer (top) and the axons towards the hilus (bottom). Scale bar, 10 µm.
We next established whether DCX expression in the human DG could be linked to other markers that in the rodent are associated with adult hippocampal neurogenesis (Complete list in
C–E, The SGZ of juvenile and adult subjects exhibits immature DCX+ cells co-expressing
N, Immature neurons express DCX together with postmitotic neuronal marker NeuN; here at age of 75 years. O, Expression of PCNA in GFAP+ cells might be indicative of ongoing stem cell activity in the SGZ but might also reflect classical astrocytic proliferation or DNA repair (see
We first examined markers for cell proliferation. PCNA expression in DCX+ cells could be detected at all ages (
Based on our previous studies in rodents and reports from the literature we next focused on nestin
Sox2 is a precursor cell marker that in rodents can be detected in a proportion of hippocampal progenitor cells
Prox1 expression, a transcription factor closely related to granule cell development in rodents
Although being glutamatergic neurons, granule cells can co-express GABA and GABA synthesizing enzymes. We detected GAD67/DCX co-localization up to 89 years of age (
Finally, we attempted a semi-quantitative assessment of neurogenesis in the human DG (including the hilar polymorphic layer) across the lifespan. We found that the density of DCX+ cells showed a log-log linear course of the regression curve over the period of life (
Comprising data from 45 subjects, between 1 day and 94 years, the number of labeled cells in the DG were plotted against the age of the individual. The regression curve shows a log-log-linear decrease (Pearson's Correlation Coefficient (PPC): -0.95102;
Research on adult hippocampal neurogenesis is often justified by the possible implications that this process might have for human cognition in health and disease. Given this claim the paucity of direct information about adult neurogenesis in the adult and aging human brain is disturbing. We here offer a large data set that provides qualitative and semi-quantitative information about neurogenesis-associated features in the human hippocampus. These data do not offer per se proof of adult neurogenesis in each particular sample but together provide valuable information about the presence of neurogenesis-associated markers in the adult human brain and their change with increasing age.
We show that during the first years of life the human DG shows an expression pattern of DCX in numerous combinations with markers of proliferation, precursor cells, maturation, and differentiation that is completely consistent with the information about hippocampal neurogenesis in rodents (
The frame indicates the stages of adult neurogenesis covered in this study: all additional markers were tested together with DCX. Marker combinations that we could detect in our samples are marked with a green check. Not all markers were found at all ages, see
This pattern changes considerable with increasing age but does so in a way that is again more or less consistent with what is known about the age-related changes in adult neurogenesis in rodents (see
Adult hippocampal neurogenesis in mice and rats shows a sequence of marker expressions that has been described in increasing detail
Presence of stem-like cells was suggested by the detection of Sox2
Because precursor cells are by definition proliferating cells markers of cell division help to substantiate the nature of the DCX-positive cells. Reif et al., who based their conclusions on adult human neurogenesis on immunohistochemistry for proliferation marker Ki67 and studied 60 samples between 25 and 68 years, did not detect positive cells in all cases and found Ki67 up to 62 years (
In rodents, the phase of DCX expression is also the phase during which cells are eliminated by cell death
DCX is associated with neurogenesis in the dentate gyrus of adult rodents but expression of DCX is not limited to the context of adult neurogenesis. The overlap with other markers corroborates the idea that the link might exist in the human dentate gyrus as well but in humans as in rodents, DCX is not a neurogenesis marker with high specificity. In the rodent hippocampus its sensitivity is high.
In the murine dentate gyrus DCX shows a nearly complete overlap with the expression of the polysialilated form of the Neural Cell Adhesion Molecule (PSA-NCAM)
Of particular interest are the round-shaped DCX-positive cells, which from their morphology and co-marker expression might relate to the DCX-positive progenitor cells, type-2b and -3 that have been described for the rodent brain. This extrapolation is speculative because further information about the exact course of adult neurogenesis would be needed. Our data might provide at first indication. More detailed analysis is hindered by the fact, however, that these cells are only a subset of all DCX-positive cells and in any given section only few markers can be tested. To describe the course of adult neurogenesis and its dynamics a study based on a cohort of cells that can be followed through the stages of development would be needed. This, however, requires the BrdU-method (or a virus-based approach), which is not readily applicable to humans.
If we take the BrdU-method as the Gold standard (which appears to be generally accepted in the field), there is currently no evidence of adult hippocampal neurogenesis in humans older than 72 years, the oldest sample in the Eriksson study. Because a new BrdU-based study in even older subjects is unlikely to be presented anytime soon, surrogate markers and cumulative supportive evidence gains weight for this age bracket. Single markers will generally not suffice to prove or disprove adult neurogenesis in a particular human sample but contextual information as offered here might help to judge these cases. The use of markers is generally affected by the many caveats associated to investigating human post mortem samples. Combination of markers will decrease the likelihood of false-positive results, but the most serious concern is that with increasing age, markers that in young age are indeed associated with neurogenesis might be increasingly indicative of degeneration and cell death. DNA-synthesis
In any case, however, and contrary to the apprehension that with increasing age the amount of non-specific labeling and false-positive marker expression might generally increase, we found a reduction in DCX expression and a reduction in marker overlap. In no case, overlaps not present at a younger age were found in old age. To our knowledge ours it the first study to undertake the analysis of so many histological markers in human samples and this marker loss by itself constitutes an interesting result. Most published studies have been concerned with the age-dependent appearance of histological features, most notably neurofibrillary tangles, senile plaques, deposition of age-pigment, or the increased proliferation of macro- and microglial cells.
Still, the subjects in our series were not healthy but died for extracerebral reasons listed in
PCNA is co-factor of DNA polymerases and is expressed during G1 and S phase of the cell cycle. PCNA is critically involved in DNA replication
We also assessed the temporal change in the number of DCX+ cells with age and found an exponential decrease, again similar to the decrease in neurogenesis in rodents
In fact we found in the present study that the panel of overlapping markers was exhaustive up to 30 to 40 years (
Stereological studies of the human DG, covering samples up to 101 years of age, determined that the number of granule cells in humans is very stable across the lifespan but varies greatly
The reduced presence of neurogenesis-associated features in the aged human hippocampus suggests that, as in rodents, neurogenesis is likely to occur on a very low scale, at least if compared to younger age. One of the intriguing questions is thus, how so few new neurons might be functionally beneficial at all. The alternative position to this approach is the theory that because in the course of evolution the ability to produce neurons in adulthood became increasingly restricted, humans are different from animals in that their hippocampus does not rely on this option to alter its neuronal network structure any longer
We have proposed models that help to explain why already very few neurons might make relevant functional contributions in the particular network situations of the hippocampus and we believe that such models also explain why postnatal and young-adult neurogenesis is necessary but increasingly dispensable with increasing age
Overall, our results suggest a pattern of adult neurogenesis in the aging human dentate gyrus that shows large similarities to rodents. Our data alone cannot prove or disprove the true presence or absence of neurogenesis at any age and the consideration of isolated samples might be misleading. Our key results lie in the fact that we see an expression pattern for key markers for adult neurogenesis also in the human brain and that this expression pattern changes with time both qualitatively and quantitatively.
Full description of the experimental procedures can be found in the supplemental material (
Autopsy cases were selected from the archive at the Department of Neuropathology, University Hospital, Freiburg, Germany, according to age, sex, postmortem interval and lack of clinical or postmortem evidence of neuropathology. Paraffin sections from 3 fetal brain tissue samples (GW11, 20 and 40), hippocampal pieces from a patient suffering from temporal lobe epilepsy and from 51 deceased persons without central nervous damage aged from 1 day to 100 years were analyzed (
Western blotting and
Standard protocols were followed. The detailed description is found in the Supplementary Material. Antibodies are listed
Supplemental material and methods.
Test of juvenile and adult human GCL specimens for potential perimortal hypoxia. A, No co-expression of DCX with the Heat Shock Protein 27 (HSP27) was detected and no signs of microglia activation (CD68) were found in the samples. B, Similarly, microglial marker Glut1 and Hypoxia-Induced Factor 1α (HIF-1α) were not increased. C, Matrix MetalloProteinases (MMP) are important for migration of young cells within established neural networks. Here, MMP9 shows strong co-labeling with DCX, whereas the same cell is not stimulated to express Vascular Endothelial Growth Factor A (VEGF-A), which would be indicative of a hypoxic milieu. D, Proliferation markers like PCNA might show expression in cells undergoing apoptosis. Here, a DCX+ cell in a 2 month-old GCL presents signs of programmed cell death by activated-Caspase-3 (Casp-3). E, Whereas apoptosis is very physiologic in neonatal age and crucial for neuronal network consolidation, the DG of a 58 years-old subject revealed no signs of degradation. The lack of PCNA- and activated caspase-3 labeling in the presented cell argues against the interpretation that DCX expression in higher age is induced by stress factors. Scale bars, 5 µm and 10 µm as indicated.
A, B, Proof of reactivities of anti activated-caspase-3 (A) and anti heat-shock protein HSP27 antibodies (B) in a glioblastoma multiforme specimen. Positive cells are devoid of DCX and PCNA immunoreactions, resp. C, Technical control incubation: Omitting primary antibodies, the incubation of serial sections with secondary antibodies alone failed to generate any specific fluorescence signal. D, E, Search for DCX expressing neuronal cells outside the hippocampus. Bipolar DCX+ cells could be detected in the parietal neocortical parenchyma (LII) (D) as well as in the temporal migratory stream (TMS) of the piriform Cortex/Amygdala (E). There are neither co-labelings with the microglial marker CD68 and the heat-shock-protein HSP27 nor with the cell type specific markers NeuN and GFAP. Scale bar length as indicated.
PSA-NCAM expresssion. PSA-NCAM is expressed in a small hippocampal SGZ cell of a 68 years-old male. DAB staining.
Estimation of PCNA+ cell densities in the DG across the entire human lifespan. Comprising data from 25 patients, aged between 1 day and 100 years (see
Doublecortin mRNA. Generation, purification and cloning of human DCX-specific cDNA inserts aimed to generate DIG-labeled cRNA probes for northern blotting and in situ hybridization. A, After bulk separation in a preparative 1.5% agarose gel, the fetal human brain DCX-PCR product (510 bp, lane 2) was recovered and in B, ligated into a pSPT19 vector (3.604 bp, lane 1), cloned and approved for correct insertion by digestion with EcoRI and HindIII resulting in a linearized vector (3.104 bp) and the DCX insert (lane 2) designed for the cRNA-DIG probe generation by in vitro transcription. C, Separation of total RNA from fetal brain in an ethidium bromide stained acrylamide gel (lane 1) showing strong fluorescence of the 18S and 28S ribosomal rRNAs. D, After blotting on nylon membrane the immobilized RNA was simultaneously hybridized with the DIG-labeled antisense probes against DCX and β-actin mRNAs and processed for visualization of the mRNA/cRNA-DIG binding sites by the enhanced chemiluminescence method. Besides the very strong signal of β-actin mRNA serving as housekeeping transcript and loading control a faint band of DCX mRNA was visible at the correct position of 9.54 kb.
Analysis of perimortal hypoxic changes.
Data of subjects included in the study.
Quantification of PCNA and GFAP co-expressing undifferentiated cells in the dentate gyrus.
List of primary antibodies.
Fluorochrome conjugated secondary antibodies and optical/technical parameters for their detection in the Leica TCS NT confocal laser scanning microscope.
We dedicate this article to the memory of Peter Eriksson. We thank Marco Prinz for generous support, Jürgen Schulte-Mönting for statistical calculations, Gesina Grothe for cell counting, Monika Hock, Christina El-Gaz, Barbara Herbstritt, Karin Strasser, and Viviana Sverdlick for excellent technical support, and Marcia Machein, Andrea Ullrich and Klaus Müller for valuable discussions.


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As reported by Censor.NET, Evropeiska Pravda obtained a copy of the letter signed by Chief of the IMF Mission to Ukraine Ron van Rooden that the Ukrainian government received on Monday, May 15.
The letter is largely devoted to the draft law No. 6220 submitted to the Ukrainian parliament. It was designed to amend the Criminal Procedure Code in order to bar NABU from investigating corruption allegations against high-ranking officials.
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BBC News
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The European Union says Turkey's measures against the education system, the judiciary and the media following the failed coup are "unacceptable". In a statement, High Representative Federica Mogherini and Commissioner Johannes Hahn said they were …
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