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DeNicola, G.M, Chen, P.-H., Mullarky, D., Sudderth, J.A., Hu, Z., Wu, D., Tang, H., Xie, Y., Asara, J.M., Huffman, K.E., Wistuba, I.I., Minna, J.D., DeBerardinis, R.J. and Cantley, L.C.
Notes: Serine/Glycine biosynthesis was examined for a panel of 79 non-small cell lung carcinomas, and each could be classified into a high-synthetic and a low-synthetic group based on expression of four enzymes involved in serine/glycine synthesis from glucose. ATF4 was identified as a key mediator of NRF2 activity for serine/glycine biosynthesis through transcriptional activation and not regulation of ATF4 translation (the latter being examined in a dual-luciferase reporter assay using Dual-Glo® Luciferase Assay System). A byproduct of the conversion of serine to glycine is production of NADPH. Seven high-synthetic cell lines exhibited a marked decrease in NADPH levels with regard to NADP+ level when the rate-limiting serine/glycine synthetic enzyme was knocked down with shRNAs with little change in NADPH/NADP+ ratios in six low-synthetic cell lines. The levels of NADPH and NADP+ were monitored with the NADP/NADPH-Glo™ Assay. Expression of the various shRNAs used in the study did not induce cytotoxicity as judged by staining with the CellTox™ Green Cytotoxicity Assay and visual examination by fluorescent microscopy. The identity of all cell lines used in this study were verified by STR analysis with a PowerPlex® System. (4715)
Expand Full Notes »
Dual-Glo® Luciferase Assay System
CellTox™ Green Express Cytotoxicity Assay
CellTox™ Green Cytotoxicity Assay
NADP/NADPH-Glo™ Assay
Expand Products »
Merhi, A., De Mees, C., Abdo, R., Alberola, J.V. and Marini, A.M.
Notes: The putative promoter and deletion mutants of the proposed promoter of the RBHG gene were cloned into the pGL3-Basic Vector for reporter assay investigation. Reporter plasmids and the pRL-TK Control Vector were cotransfected into HepG2 cells with the ViaFect™ Transfection Reagent (transfection details not provided). Forty-eight hours post-transfection, reporter activity was measured with the Dual-Glo® Luciferase Assay System using a GloMax® Instrument. (4685)
ViaFect™ Transfection Reagent
pRL-TK Vector
pGL3-Basic Vector
GloMax® 96 Microplate Luminometer
Muraoka, N., Yamakawa, H., Miyamoto, K., Sadahiro, T., Umei, T., Isomi, M., Nakashima, H., Akiyama, M., Wada, R., Inagawa, K., Nishiyama, T., Kaneda, R., Fukuda, T., Takeda, S., Tohyama, S., Hashimoto, H., Kawamura, Y., Goshima, N., Aeba, R., Yamagishi, H., Fukuda, K. and Ieda, M.
Notes: For construction of the Snai1 30 UTR reporter, the CMV promoter was subcloned into the promoterless pGL3-Basic vector upstream of the luciferase gene. A 755-bp Snai1 30 UTR fragment containing miR-133a-binding sites was amplified by PCR and subcloned into the modified pGL3-Basic vector. The activities of firefly luciferase and renilla luciferase in the control vector were determined by the Dual-Glo® Luciferase Assay System. RNA was extracted from MEFs, GMT-, GMT/miR-133-, or GMT/miR-133/ Snai1-induced aMHC-GFP+ cells, neonatal mouse heart tissues, HCFs, GMTMM-, GMTMM/miR-133-, GMTMM/miR-133/Snai1-transduced HCFs using ReliaPrep™ RNA Cell Miniprep System for gene microarray analysis. (4737)
ReliaPrep™ RNA Cell Miniprep System
Inoue, H., Hayashi, N., Matsushita, A., Xinqiong, L., Nakayama, A., Sugano, S., Jiang, C.J. and Takatsuji, H.
Notes: To understand the mechanism of Panicle blast 1 (Pb1) gene-mediated resistance to rice blast, a rice fungal disease, researchers investigated Pb1 interacted with a transcription factor involved in resistance, WRKY45 that is regulated by the ubiquitin system. To study how these proteins interacted, inner rice leaf sheaths were bombarded with gold particles coated with 0.5 µg of effector plasmid, 0.3 µg of NanoLuc® luciferase reporter and 0.1 µg of reference Renilla luciferase. After incubating overnight at 28°C, samples were ground in liquid nitrogen and reporter activities assayed using the Dual-Glo® Luciferase Reporter Assay System and Nano-Glo® Luciferase Assay System. The Renilla luciferase gene was also split into an N-terminal construct and C-terminal construct, expressed in rice protoplasts and assayed for reconstituted Renilla luciferase activity. Expression was normalized to firefly luciferase. (4510)
Nano-Glo® Luciferase Assay
pGL4.70[hRluc] Vector
Siesser, P.F., Motolese, M., Walker, M.P., Goldfarb, D., Gewain, K., Yan, F., Kulikauskas, R.M., Chien, A.J., Wordeman, L. and Major, M.B.
Notes: To better understand what roles FAM123A may play in signaling, cell behavior and human disease, HT1080 sarcoma cells were plated on MatTek dishes coated with 5mg/ml fibronectin before transfection with Venus (a yellow fluorescent protein)-tagged FAM123A or Venus-WTX, another member of the FAM123 gene family, using FuGENE® HD Transfection Reagent. The cells were imaged the next day for low-resolution analysis. For a higher magnification, dynamic analysis, HT1080 cells were plated onto Delta T dishes coated with 5mg/ml fibronectin and transfected with EGFP-FAM123A and FuGENE® HD Transfection Reagent. The next day, cell images were captured every 10 seconds. Examining the effect of silencing FAM123A, a reporter assay used the pGL4.34[luc2P/SRFRE/Hygro] Vector cotransfected with a CMV-Renilla coreporter and other effector plasmids or siRNA, and luciferase levels assessed using the Dual-Glo® Luciferase Assay System. (4246)
FuGENE® HD Transfection Reagent
pGL4.34[luc2P/SRF-RE/Hygro] Vector
Yuanzheng, H., Xu, Y., Zhang, C., Gao, X., Dykema, K.J., martin, K.R., Ke, J., Hudson, E.A., Khoo, S.K., Resau, J.H., Alberts, A.S., Mackeigan, J.P., Furge, K.A. and Xu, H.E.
Notes: Yuangheng He and colleagues asked how the weak alkaline compound chloroquine (CQ) enhances the anti-inflammatory effects of synthetic glucocorticoids like dexamethasone, which are used to treat a host of inflammatory and autoimmune diseases. In the process they explored the intersection of lysosomal degradation pathways and glucocorticoid receptor signaling. They used Dual-Glo® Luciferase Assay System to look at glucocorticoid receptor-mediated (GR) activation and repression of reporters in AD293 cells under a variety of conditions (presence or absence of CQ; stripped serum, loss of lysosome synthesis, inhibition of V-ATPase, etc). They also used HaloTag® protein fusions to observe the fate of GR populations in the presence or absence of CQ and in the presence or absence of compounds that impair proteasome function. Live-cell imaging of GR-HaloTag® protein fusions revealed a dynamic association of the GR with lysosomes. The authors showed that glucocorticoid signaling is regulated by lysosomes. (4203)
HaloTag® TMR Ligand
Thorne, C.A., Lafleur, B., Lewis, M., Hanson, A.J., Jernigan, K.K., Weaver, D.C., Huppert, K.A., Chen, T.W., Wichaidit, C., Cselenyi, C.S., Tahinci, E., Meyers, K.C., Waskow, E., Orton, D., Salic, A., Lee, L.A., Robbins, D.J., Huppert, S.S. and Lee, E.
Notes: The authors used a biochemical assay using Xenopus egg extracts to monitor degradation levels of two Wnt pathway components, Axin and ß-catenin, and identify modulators of the Wnt pathway. ß-catenin and Axin were expressed in vitro as firefly and Renilla luciferase fusion proteins, respectively, using the TNT® SP6 High-Yield Protein Expression System and shown to behave in Xenopus extracts in a similar way to wildtype proteins, which were expressed as radiolabled proteins in rabbit reticulocyte lysate. Degradation of labeled proteins was monitored by SDS polyacrylamide gel electrophoresis and autoradiography, and degradation of luciferase fusion proteins was examined using the Dual-Glo® Luciferase Assay. Using the Xenopus extract-based assay, the authors screened chemical libraries to identify two modulators of the Wnt pathway, then confirmed this inhibition in HEK 293 cells by demonstrating a decrease in ß-catenin expression with increasing concentrations of the inhibitors. This decrease in ß-catenin-Fluc levels was measured using the Steady-Glo™ Luciferase Assay, and luciferase activity was normalized to cell viability, as determined using the CellTiter-Glo® Assay. (4181)
CellTiter-Glo® Luminescent Cell Viability Assay
Rabbit Reticulocyte Lysate System, Nuclease Treated
Steady-Glo® Luciferase Assay System
TnT® SP6 High-Yield Wheat Germ Protein Expression System
Mateo, M., Carbonnelle, C., Reynard, O., Kolesnikova, L., Nemirov, K., Page, A., Volchkova, V.A. and Volchkov, V.E.
Notes: The authors used Dual-Glo® Luciferase Assay to measure a pISG54 promoter-driven firefly luciferase gene (0.5µg) and pRL-TK plasmid constitutively expressing Renilla luciferase (0.1µg), and either a plasmid (0.5µg) expressing the corresponding variants of Ebola virus (EBOV) structural protein VP24 construct (phCMV-EBOV-VP24) or empty phCMV in HEK 293T and GPC-16 transfected cells. A total of 1.1µg of DNA was transfected. Cells were stimulated with interferon (IFN) 24 hours post-transfection, harvested 16 hours later, and assayed for dual-luciferase activity. Data indicated that mutations in the V24 protein were associated with EBOV virulence but that this virulence was not linked to the IFN-antagonist function of V24 protein. (4178)
Wynendaele, J., Böhnke, A., Leucci, E., Nielsen, S.J., Lambertz, I., Hammer, S., Sbrzesny, N., Kubitza, D., Wolf, A., Gradhand, E., Balschun, K., Braicu, I., Sehouli, J., Darb-Esfahani, S., Denkert, C., Thomssen, C., Hauptmann, S., Lund, A., Marine, J.C. and Bartel, F.
Notes: The authors identified a single nucleotide polymorphism (SNP) in the 3´ untranslated region (3´ UTR) of MDM4, which promotes tumorigenesis by decreasing p53 tumor suppressor function, in ovarian cancer cells. This A to C transversion creates a putative target site for the hsa-miR-191 microRNA in the MDM4-C allele, but not the wildtype MDM4-A allele. To determine if this SNP affects MDM4 translation efficiency or mRNA stability, the authors cloned a 224-bp fragment of the MDM4 3´UTRs into the psiCHECK™-2 Vector and transfected the ovarian cancer cell line A2780 with the 224A or 224C variants of the MDM4 3´ UTR. The authors used the luciferase-based psiCHECK™-2 Vector and Dual-Glo® Luciferase Assay System to show that the C variant dramatically reduces translation efficiency and/or mRNA stability. They also assessed MDM4 expression levels in A/A, A/C and C/C ovarian cancer cells and tissues using RT-qPCR. qPCR primers for MDM4 were designed using the Plexor® Primer Design Software, and assays were performed using the Plexor® qPCR System. (4157)
psiCHECK™-2 Vector
Zhang, L.H., Kamanna, V.S., Ganji, S.H., Xiong, X-M. and Kashyap, M.L.
Notes: The authors investigated the role of pioglitazone on transcriptional regulation of the apoA-I gene and looked at the biological properties of pioglitazone-induced apoA-I-containing high-density lipoprotein particles (HDL). To investigate the biological properties of the HDL particles, the authors treated THP-1 cells with conditioned medium from HepG2 cultures treated or untreated with pioglitazone and looked at adhesion to human aortic endothelial cells (HAEC). During the experiment, HAEC viability and proliferation were monitored using the CellTiter-Glo® Luminescent Cell Viability Assay. Additionally, to determine whether pioglitazone stimulates apoA-I transcription, a luciferase reporter construct was made containing the apoA-I gene promoter. Transfected cells were treated with pioglitazone, and luciferase expression was monitored using the Dual-Luciferase® Reporter Assay System. (4173)
Ghisolfi, L., Calastretti, A., Franzi, S., Canti, G., Donnini, M., Capaccioli, S., Nicolin, A. and Bevilacqua, A.
Notes: To examine post-transcriptional regulation of B cell lymphoma (bcl)-2 mRNA in human cell lines, the bcl-2 open reading frame was cloned into the pCI-neo Mammalian Expression Vector with a FLAG tag. This construct was transfected into U2OS human osteosarcoma cells, lysed, immunoprecipitated on Anti-FLAG-coated beads and analyzed by SDS-PAGE. A 260bp fragment containing the human c-myc 3´UTR adenine-uridine(AU)-rich element (ARE) was cloned into the pGL4.71 [hRlucP] Vector above to produce the pGL4.71PmARE plasmid. For transient expression, SK-N-BE and HEK293 cells in 96-well plates were cotransfected with 200ng of the pGL4.71 constructs (carrying c-myc or bcl-2 ARE) and 200ng of the pGL3-Control Vector. Luciferase expression was assessed using the Dual-Glo® Luciferase Reporter Assay System. HEK293 cells were also stably cotransfected with the same pGL4.71 constructs and a vector carrying G418 resistance. Renilla luciferase expression was assessed and clones chosen with similar expression levels. (4071)
pCI-neo Mammalian Expression Vector
pGL4.71[hRlucP] Vector
Zheng, Y., Ritzenthaler, J.D., Sun, X., Roman, J. and Han, S.
Notes: In this paper, the role of prostaglandin E2 (PGE2) stimulation of integrin-linked kinase (ILK) in human lung carcinoma was explored. Mutations of Sp1 and NF-κB cis-acting elements in an ILK promoter-pGL3-Basic Vector construct were created using the GeneEditor™ in vitro Site-Directed Mutagenesis System. The mutations were confirmed via sequencing. Human non–small cell lung carcinoma (NSCLC) cells were plated at a density of 5 × 105 cells per well in six-well plates and transfected with 2µg of ILK promoter reporter vectors with or without 0.2µg of the phRL-TK Renilla Luciferase Reporter Vector. After 24 hours, the transfected cells were exposed to PGE2 and the cells lysed for assessment using the Dual-Luciferase® Reporter Assay System. NSCLC cells were transfected with inactive (ILK-S343A) and superactive ILK (ILK-S343D) cDNA, incubated for 24 hours, treated with or without exogenous PGE2 or with an Sp1 inhibitor for 2 hours. The numbers of viable cells were measured using the CellTiter-Glo® Luminescent Cell Viability Assay. (4026)
Lin, W., Wu, J., Dong, H., Bouck, D., Zeng, F.Y and Chen, T.
Notes: HepG2 cells in a T-25 culture flask (3 million cells at 70ndash;80% confluency) were transfected with CYP3A4 reporter plasmid (a pGL3 reporter construct) and a CMV-Renilla control plasmid (a total of 2.5µg of plasmid mix was used) using FuGENE® 6 Reagent for transient transfection assays. Reporter activities were measured using the Dual-Glo® Luciferase Assay System. (4268)
FuGENE® 6 Transfection Reagent
pGL3-Control Vector
pGL3-Promoter Vector
pGL3-Enhancer Vector
pRL-CMV Vector
Nagase, T., Yamakawa, H., Tadokoro, S., Nakajima, D., Inoue, S., Yamaguchi, K., Itokawa, Y., Kikuno, R.F., Koga, H. and Ohara, O.
Notes: These authors used the Flexi® Vector System to prepare ORF clones encoding 1929 human genes and to transfer a subset of these clones to various expression vectors for further analysis. They created HaloTag® fusion proteins and examined expression of these proteins in vitro and in COS7 and HEK293 cells. They also performed comparisons between the Flexi® System and Gateway® cloning system, specifically examining the effects of flanking sequences on protein expression in in vitro translation systems and confirming that the cellular localization of the HaloTag® fusion proteins was consistent with results obtained using GFP-fusions. (3800)
Anti-HaloTag® pAb
Flexi® System, Entry/Transfer
Flexi® System, Transfer
GloMax® 96 Microplate Luminometer w/Dual Injectors
Monster Green® Fluorescent Protein phMGFP Vector
pF1K T7 Flexi® Vector
pGL4.10[luc2] Vector
T7 RiboMAX™ Express Large Scale RNA Production System
Wizard® SV 96 PCR Clean-Up System
Fan, F. and Wood, K.V.
Notes: The authors of this paper review bioluminescent assay technologies, discussing HTS reporter, cell-based and luciferase biosensor assays. They divide luminescent assays into three basic categories: assays that measure ATP concentration (cell viability and kinase assays), assays that measure changes in luciferase levels (reporter assays, GPCR assays), and assays that measure changes in luciferin levels (protease [including caspase], P450 and MAO assays). (3737)
BacTiter-Glo™ Microbial Cell Viability Assay
Bright-Glo™ Luciferase Assay System
Calpain-Glo™ Protease Assay
cAMP-Glo™ Assay
Caspase-Glo® 2 Assay
Caspase-Glo® 3/7 Assay
Caspase-Glo® 6 Assay
Caspase-Glo® 8 Assay
Caspase-Glo® 9 Assay
Dual-Luciferase® Reporter 1000 Assay System
Dual-Luciferase® Reporter Assay System
Dual-Luciferase® Reporter Assay System 10-Pack
Kinase-Glo® Luminescent Kinase Assay
Kinase-Glo® Plus Luminescent Kinase Assay
MAO-Glo™ Assay
P450-Glo™ CYP1A1 Assay
P450-Glo™ CYP1A2 Assay
P450-Glo™ CYP1A2 Screening System
P450-Glo™ CYP1B1 Assay
P450-Glo™ CYP2C19 Assay
P450-Glo™ CYP2C19 Screening System
P450-Glo™ CYP2C8 Assay
P450-Glo™ CYP2C9 Assay
P450-Glo™ CYP2C9 Screening System
P450-Glo™ CYP2D6 Assay
P450-Glo™ CYP2D6 Screening System
P450-Glo™ CYP3A4 Assay
P450-Glo™ CYP3A4 Assay (Luciferin-PFBE)Cell-Based/Biochemical Assay
P450-Glo™ CYP3A4 Assay (Luciferin-PPXE)DMSO-Tolerant Assay
P450-Glo™ CYP3A4 Screening System
P450-Glo™ CYP3A4 Screening System (Luciferin-PPXE)DMSO-Tolerant Assay
P450-Glo™ CYP3A7 Assay
Renilla Luciferase Assay System
Belov, G.A., Altan-Bonnet, N., Kovtunovych, G., Jackson, C.L., Lippincott-Schwartz, J. and Ehrenfeld, E.
Notes: In this study, the Renilla luciferase gene from the phRL-CMV Vector was incorporated into polioviral RNA in place of a structural protein-coding region. Renilla luciferase activity was then monitored as a convenient indicator of viral replication. Specifically, these authors tested whether the guanine nucleotide exchange factors GBF1 and BIG2 could rescue brefeldin A (BFA)-induced inhibition of polioviral replication in HeLa cells. Cells were transfected with plasmids encoding either GBF1 or BIG2, together with the firefly-luciferase-encoding pGL4.13 Vector, which was used as a control to monitor transfection efficiency. Eighteen hours post-transfection, the cells were transfected with polioviral replicon RNA containing the Renilla luciferase gene. One hour later, normal growth medium containing EnduRen® Live Cell Substrate and either BFA or solvent alone, was added and Renilla luciferase activity was monitored at hourly intervals for 7 hours. Cells were then lysed and firefly luciferase activity was measured to assess transfection efficiency using the Dual-Glo® Luciferase Assay System System. In the presence of BFA, GBF1 was able to partially rescue viral replication. In the presence of BIG2 and BFA, no viral replication occurred. (3562)
EnduRen™ Live Cell Substrate
pGL4.13[luc2/SV40] Vector
Betzi S, Restouin A, Opi S, Arold ST, Parrot I, Guerlesquin F, Morelli X, Collette Y.
Notes: The authors wanted to screen inhibitory compounds for the HIV-1 accessory protein Nef using both computer modeling and experimental methods. Using a structure-based program for the SH3 binding surface of Nef, drug compounds were screened in silico and then further analyzed using a cell-based assay. The Nef gene and SH3 domain were cloned into the pACT and pBIND Vectors of the CheckMate™ Mammalian Two-Hybrid System, transfected into COS-7 cells, and 18 hours later, the cells exposed to potential inhibitors. After 24 hours, luciferase activity was assessed using either the Dual-Glo™ or the Steady-Glo® Luciferase Assay Systems. (3751)
CheckMate™ Mammalian Two-Hybrid System
Zhuang, H. and Matsunami, H.
Notes: To explore the role that accessory proteins may play in successfully expressing odorant receptors (ORs) on the cell surface of heterologous cells, the authors explored cotransfecting receptor-transporting proteins RTP1S, Ric8b and Gαolf with an N-terminally tagged OR to generate a functional and ligand-specific expression system. Three tags (Rho, FLAG or HA) were inserted into the NheI and EcoRI sites of the pCI Mammalian Expression Vector. Amplified OR orfs were cloned into the MluI and NotI sites of the tagged pCI Mammalian Expression Vector. The accessory proteins were subcloned into HA-pCI (RTP1S) or pCI (RTP1S, Ric8b, Hsc70t and Gαolf). For cotransfection, the N-terminal tagged OR vectors (0.8µg) and the assessory proteins (individually or in combination; 0.8µg) were transfected into HEK293T or Hana3A cells. The transfected cells were then subjected to immunocytochemistry, live-cell surface staining, permeabilized staining or FACS analysis. The Dual-Glo™ Luciferase Assay System was used to assess OR activation via CRE elements on a firefly luciferase vector. pRL-SV40 Vector was the internal control for cell viability and transfection efficiency. HEK293T or Hana3A cells were plated on 96-well plates, transfected with 1µg of CRE-Luc vector, 1µg of pRL-SV40 Vector, 5µg of OR and 1µg total for all accessory proteins (0.25µg each protein with pCI Mammalian Expression Vector to keep amount of plasmid constant). Twenty-four hours posttransfection, the medium was changed to CD293 chemically defined medium, incubated for 30 minutes at 37°C then replaced with 25µl of odorant solution in CD293 for a second incubation of 4 hours at 4°C. Then the reporter protein expression levels were measured by luminescence. (3687)
pCI Mammalian Expression Vector
pRL-SV40 Vector
Campbell, D.B., Sufcliffe, J.S., Ebert, P.J., Militerni, R., Bravaccio, C., Trillo, S., Elia, M., Schneider, C., Melmed, R., Sacco, R., Persico, A.M. and Levitt, P.
Notes: In this family-based study of 1,231 autism cases, a genetic association of a common C allele in the promoter of the MET gene and autism was identified. Initial sequencing of the MET genes from 86 individuals with autism was used to identify several candidate variants in the MET promoter and 3´ untranslated region. Family-based analyses were then performed to determine whether an association could be demonstrated between any of these variants and autism. A G/C variant 20bp 5´ of the MET transcription start site was found to be overrepresented among individuals with autism, particularly among families where more than on child was affected. Once the candidate variant was identified, the effect of the G/C change on transcription of the MET gene was investigated in a reporter assay. Two 762 bp fragments of the MET promoter region, differing only in the G/C nucleotide, were cloned into pGL4.10 firefly luciferase reporter vectors. These vectors were then transfected into mouse neural cell lines, and luciferase production was monitored using the Dual-Glo™ Assay. The construct containing the C allele produced less than half of the luciferase activity of construct containing the G allele. (3579)
Radhakrishnan, S.K., Bhat, U.G., Hughes, D.E., Wang, I.C., Costa, R.H. and Gartel, A.L.
Notes: To screen a compound library for an inhibitor of FoxM1 transcriptional activity, U2OS cells with doxycyclin-inducible FoxM1-green fluorescent protein (GFP) fusion protein were stably transfected with a firefly luciferase construct driven by a 6× FoxM1 responsive promoter and the pRL-CMV Vector. These cells were grown overnight in 96-well plates, treated with doxycyclin and test compounds. Firefly and Renilla luciferase expression was assessed using the Dual-Glo™ Luciferase Assay System. (3730)
Ullah, M.S., Davies, A.J. and Halestrap, A.P.
Notes: Monocarboxylate transporters (MCT) transport lactic acid across the cell membrane. The promoters of 4 MCT family members (MCT1, MCT2, MCT3 and MCT4), were amplified by PCR and cloned into the pGEM®-T Easy Vector. The sequences were confirmed, and the promoters were cloned into the pGL3-Basic Vector. The Dual-Glo™ Luciferase Assay System was used to quantitate promoter activity under basal and hypoxic conditions in HeLa cells. The pRL-SV40 Vector was used to normalize for differences in transfection efficiency. (3463)
pGEM®-T Easy Vector System I
pGEM®-T Easy Vector System II
Martins, G.A., Hutchins, A.S. and Reiner, S.L.
Notes: To determine the potential role that T-bet, a T-box transcription factor that specifies Th1 lineage commitment, and Hlx, a homeobox gene, may have in helper T cell differentiation, the physical interaction between the two proteins was tested using the CheckMate™ Mammalian Two-Hybrid System. The pACT-T-bet and pBIND-Hlx fusion vectors were co-transfected with pG5luc into 293T cells. After 48 hours, the firefly and Renilla luciferase activities were measured using the Dual-Glo® Luciferase Assay System. (3493)
Chen, Q.Y. and Jackson, N.
Notes: These authors demonstrated that the human CD1D gene has distal and proximal TATA boxless promoter sequences. Distal and proximal promoters to CD1D were cloned into the pGL3-Basic Vector to create reporter constructs. One construct contained the entire 4,986 base pair region containing distal and proximal CD1D promoter. Transient transfections were performed using 5 x 105 Jurkat cells in 24-well plates, 0.8 μg of pGL3 Basic Vector with the insert of interest, and 30ng of pRL-CMV Vector as a transfection normalization control. The Dual-Glo™ Luciferase Assay System was used to assay luciferase activities. (3061)
Verge´, V., VonLanthenm, M., Masson, J.M., Trachsel, H., and Altmann, M.
Notes: Researchers cloned the Photinus and Renilla luciferase ORFs into the pSP64 Poly(A) Vector to create a dual-reporter vector named SP6P. A similar vector, SP6R.4G(-508/-3).P, was created in which a 5´ untranslated region from the Saccharomyces cerevisiae TIF4631 gene was cloned between the two reporter genes. These two vectors were used to transform yeast strains. The resultant transformants were lysed using Passive Lysis Buffer and a modified lysis procedure. Lysates were analyzed for luciferase activities using the Dual-Luciferase® Reporter Assay System and a TD20/20 luminometer. The researchers also cloned and sequenced the 5´ untranslated region of TIF4631 by using a RACE-PCR technique followed by cloning the PCR amplimers into the pGEM®-T Vector. (2845)
Passive Lysis 5X Buffer
pGEM®-T Vector System I
pGEM®-T Vector System II
pSP64 Poly(A) Vector
Shirasawa, S., Harada, H., Furugaki, K., Akamizu, T., Ishikawa, N., Ito, K., Ito, K., Tamai, H., Kuma, K., Kubota, S., Hiratani, H., Tsuchiya, T., Baba, I., Ishikawa, M., Tanaka, M., Sakai, K., Aoki, M., Yamamoto, K. and Sasazuki, T.
Notes: Real-time TaqMan® amplification reactions were cleaned up using the Wizard® MagneSil® Sequencing Reaction Clean-Up System. The purified products were then used in sequencing reactions. This paper also describes use of the Dual-Luciferase® Reporter Assay System to analyze HEK293 cells transfected with a pGL3-Enhancer vector construct. (3181)
Wizard® MagneSil® Sequencing Reaction Clean-Up System
Wizard® MagneSil® Sequencing Reaction Clean-Up System, HTP1


text:
Garcia gives White Sox walk-off victory in damp finale
Ninth-inning single plates winner; Danks strikes out 10 over 7 2/3
CHICAGO -- White Sox manager Robin Ventura sent an atypical and unsung pinch-hitter to the plate at the most critical stage of the series finale between his team and the Angels on Wednesday night at U.S. Cellular Field. Leury Garcia stepped into the batter's box during a tie game in the ninth with just one hit in his previous 25 plate appearances.
But on a 2-1 fastball, the second-year shortstop lined the swiftest hit of a cold, rainy night that brought home Alejandro De Aza from third to seal a 3-2 victory -- the South Siders' first of the season over the Angels.
"I think he was surprised in that spot to be pinch-hitting," Ventura said. "But he's going to make some contact. … That's a part of the game where you're really looking at contact, the way the field was, the conditions and everything."
Added Garcia: "It's kind of tough when you don't play every day, but you've got to still work and still try to get better every day and be ready for a situation."
With De Aza on third and Conor Gillaspie on first, Ventura noted that a squeeze was certainly on the table, citing the swampy field conditions and sitting with just one out. The Angels, meanwhile, went to a five-man infield.
"You're always going to go after guys, regardless of the situation," Angels pitcher Mike Morin said. "I'm not timid or shy or scared of anyone. ... I was trying to get a ground ball. A lot of my offspeed pitches usually generate fly balls. But I was trying to get a ground ball."
As De Aza raced home, the White Sox dugout energetically emerged to greet the giddy Garcia, and celebrate their seventh walk-off win of the season.
"It's fun," a beaming Garcia said after the game. "Awesome. A lot of fun."
The night was shaping up as another addition to the resurging saga of John Danks, who tied a career high with 10 strikeouts and was on the verge of his fifth win in six starts.
Danks, who recovered from a muddled May, during which he allowed 23 runs and 41 hits while going 1-4 in six starts, looked worthy of a hypothetical Comeback Player of the Month Award after going 4-1 in June with a 3.41 ERA.
But a pair of Angels homers by David Freese in the seventh and Josh Hamilton in the eighth prodded a pitching change to Zach Putnam (3-1), who earned the win with no hits allowed in 1 1/3 innings.
Ventura said he felt confident keeping Danks on the mound despite reaching 107 pitches through the seventh.
"He had gone through that part of the lineup and done pretty well," Ventura said. "You take what you've seen tonight and put it out there. He felt strong, talking to him in between the seventh and eighth, he felt great. So it's not an easy part of the lineup to go through, but the way he was pitching tonight and previously how he had gone through that part of the lineup, you give him a shot at it. I felt that was the best way to go."
Angels starting pitcher Tyler Skaggs, making his first appearance since June 5 after a stint on the disabled list, was the hot hand early. Skaggs was perfect through three innings, but loaded the bases in the span of just 12 pitches in the fourth and allowed the White Sox to pounce with a two-run double by Alexei Ramirez that gave Chicago just its second lead of the series.
Gordon Beckham bounced back from an 0-for-4 outing on Tuesday with a pair of hits, including a double with one on that preceeded Ramirez's RBIs. Beckham would've also had his eighth home run had Hamilton not yanked the ball back into play as it elevated just inches over left-field wall in the first.
"It is what it is," Beckham said. "[Albert] Pujols hit a ball pretty good, too and it didn't go out. Guess it wasn't really carrying to left as it was to right."
Record-setting Jose Abreu continued his historic season by extending his hit streak to 17 games, tying Ramirez for the White Sox season high.
Chicago turns to an off-day Thursday having won four of its last six, and will open a three-game weekend series against the Mariners on Friday.
Daniel Kramer is an associate reporter for MLB.com This story was not subject to the approval of Major League Baseball or its clubs.

subset: commoncrawl


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The CMSM is a certificate in middle-school mathematics. This certificate is designed for school personnel seeking advanced study in mathematics content appropriate for teaching mathematics at the middle-school level. Neither an undergraduate major or minor in mathematics is required for admission to this certificate program. This MHEC-approved certificate is awarded upon completion of an approved program of study consisting of hands-on pedagogically innovative mathematics content courses.
Note: This certificate is not a teaching certificate. The awarding of this CMSM certificate does not carry certification to teach.
The requirements for the CMSM are listed in the CMSM checklist.
Link to the checklist for the CMSM certificate.
Here are the steps to follow to be admitted to the certificate program:
Apply for admission to graduate study by submitting a completed Application for Graduate Program Admission, with the application fee, to the Office of Admissions. On that application, under "INTENDED PLAN OF STUDY," be sure to check the box for Math - Post-Baccalaureate Certificate in Middle School Mathematics. The application for admission is available at http://www.salisbury.edu/gsr/gradstudies/admissions.html.
Have official transcripts sent to the Office of Admissions from EVERY college and university attended.
If you are seeking in-state tuition, submit a completed Residency/Domicile Information form to the Office of Admissions. That form is also available on the Office of Admissions' web site. http://www.salisbury.edu/admissions/
When you have been admitted for graduate study, the Mathematics and Computer Science Department will send you a checklist for the CMSM certificate and you will be assigned an advisor who will help you plan your course of study.
You may contact the department regarding the CMSM at Department of Mathematics & Computer Science, Graduate Study, Salisbury University, 1101 Camden Avenue, Salisbury, MD 21801 or by at phone at 410-543-6140.
Upon approaching completion of the CMSM program, here are some steps to follow:
Students admitted and matriculated into a graduate program must complete at least one SU graduate course in each calendar year (including fall, winter, spring, and summer terms) to maintain their admitted status. Students who fail to meet that requirement and wish to re-enter the graduate program must request to be reactivated through the Registrar's Office.
Be aware that it is the responsibility of the individual graduate student to check with his/her faculty advisor to make sure that all certificate requirements have been fulfilled.
Link to a two-year schedule of anticipated offerings of graduate courses.
If you have questions regarding the CMSM program, please contact the Graduate Office of the Department of Mathematics and Computer Science at 410-543-6140. You may also contact Dr. Jennifer Bergner, the MSME/CMSM Program Director, via e-mail at firstname.lastname@example.com.
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David Koma Spring 2012
Judging by the fashion we've seen over the past few days, it's safe to say that London has picked up the baton for prints where New York left off. Eschewing the trend, designer David Koma stripped the idea of pattern down to the bone for spring 2012 (or should that be the skin?) and used Polynesian tattoos as the template for his designs. Long-sleeved devoré tees and pants were spliced with clear panels that cast windows along arms and legs. Koma opened the show with a series of dazzling all-white looks, gradually slipping in layers of bright color; one pink-infused ensemble in particular was a convincing and wearable example of how to do body-con with transparency. Backstage Koma said that he'd given himself more time than he usually would to design the collection, and it clearly gave him time to cram his mood board with an abundance of references. Those hybrid leather skirts owed as much to the Scottish highlands as they did to the tribesmen of Zululand or to traditional hula dancers. Koma worked with jewelry designer Sarah Angold to create three-dimensional Perspex pieces to line the pleats of his dresses, a nod, he said, to Pearly King and Queens. In his hands that old-school East London luster found a techno-rave future, and it was easy to imagine the shine of those iridescent discs under the light of London's clubland. Unfortunately those heavy embellishments often made it difficult for some of the models to walk down the runway, and some were reduced to a snail's pace. Indeed it would have been wise for Koma to pare back some of the weightier trimmings, and in turn given the stronger elements in his collection room to breathe.


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SEMI is engaged in every major manufacturing region in the world and maintains offices in Bangalore, Beijing, Brussels, Hsinchu, Moscow, San Jose, Seoul, Shanghai, Singapore, Tokyo, and Washington, D.C. Primary activities include conferences and trade shows, international standards development, public policy, market research, workforce development, environmental, health and safety (EHS) other industry advocacy. Much of this work is accomplished through engagement of regional volunteer committees.
SEMI Americas
SEMI Americas has offices in Washington, D.C. and San Jose, California and regional Chapters serving members locally in the U.S.
SEMI China
SEMI China serves more than 50,000 local people from Semiconductor, Flat Panel Display and PV industries and continuously advances the growth and prosperity of SEMI members and these industries in China through diversified products and services. Over the past two decades, SEMI members has paralleled China's explosive development and enjoyed the enormous growing opportunity in China. SEMI China has offices in both Shanghai and Beijing.
SEMI Europe
SEMI Europe, with the help of its international staff, supports its 244 European members in over 21 countries across a diversified scope of activities. They include exhibitions and conferences, standards, member advocacy and industry government relations. Over 60 events/meetings are organized annually by SEMI Europe, engaging with over 14.000 industry professionals. 135 industry executives are involved in 11 committees. The SEMI Europe Newsletter is the primary communication tool, with a monthly distribution to 13.500 readers.
SEMI India
SEMI India's office is located in Bangalore and supports member interest in India's burgeoning microelectronic and PV manufacturing supply chains.
SEMI Japan
In February 1985, SEMI opened a permanent office in Tokyo. Representing the diverse views of its membership on the global stage, SEMI Japan focuses on promoting exchange among professionals, experts and manufacturers from all over the world.
SEMI Korea
SEMI Korea office is located at Seoul, Korea.
SEMI Moscow
SEMI Moscow office was established in 1992 to facilitate mutual business interests between SEMI member companies and the semiconductor industry in the Commonwealth of Independent States (CIS).
SEMI Southeast Asia
SEMI Singapore/Southeast Asia was established in 1993 in the same year the SEMICON venue was hosted in Singapore.
SEMI Taiwan
The SEMI Taiwan officially opened its office in Hsinchu in January 1996 to serve its memberships local and subsidiary offices.


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Dec. 13-19
Martin Doors Food Drive
Dec. 13-19
Martin Doors Food Drive
Employees, customers and members of the community are invited to participate. Martin Doors is partnering with the Utah Food Bank.
When • Monday-Friday, Dec. 13-19, 8 a.m.-5 p.m.
Where • Martin Door Manufacturing, 2828 S. 900 West, South Salt Lake
Dexterity Salon Fundraiser for Candy Cane Corner
Dexterity Salon is asking clients and the community to donate new, unwrapped items including clothing for all ages, linens, household appliances and toys. Visit dexteritysalon.com/community for information.
When • Monday-Friday, Dec. 13-19, 10 a.m.-6 p.m.
Where • Dexterity Salon, 777 E. 300 South, Salt Lake City
Candy Cane Corner Holiday Store Opening
The 18th annual Candy Cane Corner holiday store will open for low-income families and individuals who are participating in programs at The Road Home, Volunteers of America, Utah, and the YWCA. Candy Cane Corner is a dignified and empowering opportunity for low-income families to select new holiday gifts for themselves and their families. Donations will be accepted starting noon every day until Dec. 23. The complete wish list can be found at www.candycanecornerslc.org.
When • Monday-Friday, Dec. 13-19, noon-7 p.m., Saturday-Sunday, Dec. 15-16, noon-5 p.m.
Where • Candy Cane Corner, 502 W. 300 South, Salt Lake City
Tickets • Free
Holiday Tours of the Govenor's Mansion
The Utah Heritage Foundation will hold free tours of the Governor's Mansion during the holiday season. More information is available at www.utahheritagefoundation.org.
When • Thursday, Dec. 13, from 2-4 p.m., Tuesday, Dec. 18, 2-4 p.m.
Where • Governor's Mansion, 603 E. South Temple, Salt Lake City
Tickets • Free
Christmas Jam 3
"Christmas Jam" is a yearly concert benefiting The Road Home organization. Visit www.christmasjam.org for more information.
When • Thursday-Friday, Dec. 13-14, 7-9 p.m., Saturday, Dec. 15, 3 p.m., 7 p.m.
Where • One Mind Studio, 216 Paxton Ave., Salt Lake City
Tickets • $10; $15 day of show, children under 12 free with paying adult, $5 each extra child
Winter Farmers Market
The Utah State Fairpark will host the Winter Farmers Market the first and third Saturday of each month ending March 16 in the Deseret Building.
Where • Utah State Fairpark, 155 N. 1000 West, Salt Lake City
Tickets • Free; visit www.utahstatefair.com/wintermarket


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Today sees the publication of Credit Suisse's annual Global Investment Returns Yearbook, a mammoth piece of research into global long-run returns overseen by the London Business School academics Elroy Dimson, Paul Marsh and Mike Staunton. It is an invaluable resource, and this blog is likely to mine its contents for some days to come.
Revisions for this year help ram home one quick and spectacular lesson from history. Baron Nathan Rothschild is widely believed (wrongly, according to historian Niall Ferguson) to have said that you should "buy when there's blood in the streets".
Much of the time this advice works. Buying into Japan or Germany after the second world war would have worked out extremely well, and the greatest buying opportunities almost by definition come when it seems almost mad to buy.
But the aphorism is not infallible. This year, the academics tried to address their concern that their global stock market index suffered from "survivorship bias". So they have recalculated them including three new countries that were not previously covered in their attempts to calculated the global equity risk premium: China, Russia and Austria. Adding these nations hugely changes the perception of long-term risk.
Let's start in Russia. Any bold contrarians who decided in the late 19th century to bet on Tsarist Russia to outperform the US for the long-term, and held on even during the great political unrest of the attempted revolution of 1905, would for a long time have looked very clever.
The chart compares the St Petersburg stock exchange's composite index performance with that of New York. After 1917, of course, the value of any equity investment in Russia was wiped out. This might appear to be an exceptional example. But it is not. China also had a revolution that led to the closing down of its stock market (and of capitalism for a while), and that happened within living memory. On the eve of the second world war, China's returns looked very healthy. With the arrival of Mao, shares went to zero (and international investors have had a rough ride even since Chinese stock markets reopened).
Using the MSCI China index, covering stocks available to international investors, those who bought in 1993 have actually lost money. But Chinese stock markets have been recovering recently. And, deliciously for those who like historical ironies, the Shanghai Composite, the main domestic index, bottomed last year at 1949, the year of the revolution. Read more


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It's that time of year again, time for the Fourth Mavic Trans-Provence! This year for the first time we are proud to have as our title sponsor Mavic along side our co-sponsors of Fox and their UK distributor Mojo, Cannondale bikes and North Ridge outdoor camping and apparel. We're also pleased to have the kind support of AndyStand and Cool Bus.
For those that don't know, Mavic Trans-Provence is the only multi-day gravity-orientated itinerant MTB enduro in the world. Seven days, 310km of riding 15,000 metres of descending 10,000 metres of climbing, each day has an uplift shuttle at some point, and 26 Special Stages.
This year, 70 riders from around the world are going to test themselves against some of the hardest terrain ever raced on. From Gap in Northern Provence to Monaco on the Mediterranean coast, riders of every discipline from World Cup downhill to world cup cross country mix with the new enduro specialists, first time racers and some just there for the adventure.All of them in their own way, whether pro or amateur, will be racing against the clock, against themselves and against each other. The Winner will have the best combination of technical riding ability and the fitness to take them over the seven gruelling days.
Returning veterans from last year and some would say with things to prove, we have:
Mark Weir (4th overall last year)
Nico Vouilloz (2nd overall last year )
Ben Cruz
Last year's winner and Enduro specialist Jerome Clementz from France is back to defend his title
To take us through the week, a competitor from last year (3rd!) and another retired World Champion Downhiller and all-round good guy Fabien Barel will be with us to chat to riders, check out bikes and give us his in depth of knowledge of all things racing and to chat with this years competitors throughout the week.
The racing begins Sunday with the first of many video installment on your favourite MTB site on Monday. It's going to be an amazing week of racing… stay tuned !


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Follow the white rabbit down the hole into our Wonderland. You won't find Alice or The Mad Hatter, but you will find new friends, pretty tags, & fun activities.
wonderland, tags, artists, #taggers, copyright, safe, signature, challenges, games, friends, activities, snagger, request, contests, winners, prizes
A place for Taggers and non Taggers alike to get together and enjoy their hooby - TAGS
bag-a-snag, place, #taggers, alike, together, enjoy, their, hooby, tags
A relaxing forum for all scrappers, taggers, tubers and those who love to collect tags and join in the fun with a heap of freebies, competitions and challenges thrown in for good measure.
free, tubes, scrapkits, tags, templates, signatures, extras, #taggers, competitions, scraps, clusters, challenges, bitsnbobs, bits, bobs, games, friends, friendly, signature, forum
Free forum : A friendly drama free psp forum to share your love of tagging. Daily games, weekly challenges, and friends to make.
free, forum, tagging, scrapping, photoshop, friendly, drama, share, your, love, tagging., daily, games, weekly, challenges, friends, make
New Tag Board. Taggers Welcome, new or old. We have awesome tags here. And lots of fun. Come join us.
Free forum : We are a tagger & snagger friendly community. We are drama free at all time. Participation is a requirement.
#taggers, realm, tagger, snagger, friendly, tagging, community, drama, free, challenges, games, contest, tags, snags, graphic's, prizes
The Sav is Back! Welcome to Savage Island where you can kick back and relax. This is a place for both taggers and non-taggers to escape from reality. Welcome!
savage, island, back!, kick, relax, place, #taggers, non-taggers, escape, reality, welcome!
A group to have fun with PSP.
sinful, pleasures, photoshop, graphics, group, signatures, #taggers, scrapbook
Digital Art and Products by Angela Hobbs. ~ DIGITAL ART ~ SCRAP KITS ~ SOFTWARE ~ TUTORIALS ~ FREEBIES
digi, scrap, cafe, graphics, #taggers, digital, kits, scrapbooking, artists, designers, resources, community, social, challenges, contests, tutorials
Free forum : Offering The Best Tagger's Forum That's Just A little Different Than The Rest
free, forum, #taggers, tavern, tagger, forumotion, paint, shop, photoshop, weekly, challenges, monthly, contest, wwo's, graphic's, designers, tuts, tagging, resources, friendly, drama
The Tagger's Den forum is dedicated to encouraging the free exchange of ideas and knowledge and to expanding the creativity of all it's members, taggers, designers and artists.
#taggers, signatures, tubes, scrapkits, paint, shop, photoshop
The Best Tagger's Forum That's Just A little Different Than The Rest
best, #taggers, forum, drame, copyright, compliant, friendly, tagger's, that's, just, little, different, than, rest
Free forum : TaggersInnEvents
A Place for taggers and non taggers to come together and take challenges and forum games
creative, cavern, place, #taggers, challenges, games
tags, snags, signatures, graphics, avatars, challenges, contest, win, tubes, fun, chat, non taggers, taggers, tagging, psp, ps, games, drama free at Taggers Gone Mad.
tags, snags, #taggers, tagging, challenges, contests, group, forum, games, chat, tubes, drama, free, contest
Free forum : A relaxing forum for all scrappers, taggers, tubers and those who love to collect tags and join in the fun with a heap of freebies, competitions, challenges & more
unique, relaxing, scrappers, #taggers, tubers, love, collect, tags, join, heap, freebies, competitions, challenges
Welcome to Creative Cafe - where Taggers and Friends can come play, share, and learn.
free, forum, creative, cafe, welcome, where, #taggers, friends, scrappers, come, play, share, learn, paint, shop, tubes, tags, scrapping, siggys, siggies
Free forum : Taggers of Anarchy is an exciting place to put your creative skill to work. Taggers and non taggers are welcome. See you at TOA.
#taggers, anarchy, exciting, place, creative, graphics, photo, shop, gifts., auctions, animation
Free forum : Looking for a year round game of secret Santa using sig tags???


text:
MyCollege is the college portal that includes registration, admissions, financial aid and other student information. Log in at mycollege.sunybroome.edu for the campus news of the day, such as campus closings for bad weather. Find out what's going on before you leave home.You can check your grades, request your College transcripts, as well as gain access to other College services. Finally, you can customize the look of your own page to provide you with news, sports, weather, and links of your choice.
How will I access MyCollege?
Here's what to expect - First time user info:
Once you are signed in, you will see a series of links and special tabs leading to a variety of information. Select the "Student" tab to view all your student information.
Having trouble logging in or getting set up? Contact the HelpDesk:
Or visit Information Technology Services in Business 121 Phone: 607-778-5011
You are a:
Quick links


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NJ Residents Helping Filipino Typhoon Victims [AUDIO]
After Typhoon Haiyan ripped through the Philippines a few days ago, the death toll continues to rise, and it may climb to more than 10,000.
Many New Jersey residents are wondering what's the best way to lend a helping hand to the survivors.
"If you want to help, the best way is to make a financial donation, this way the Red Cross can purchase exactly what's needed for the disaster relief operation," said the Communications Officer for the American Red Cross, North Jersey Region, Diane Concannon.
"It enables us to buy the supplies close to the disaster site," she said, "and avoids the delays and transportation costs in getting basic necessities to the people who need it most."
Concannon pointed out you can make a contribution by going to www.redcross.org, or by calling 1-800-REDCROSS.
If people in New Jersey have loved ones in the Philippines and they have not been able to get in touch with them, Concannon said they should remember there is a lot of difficulty with communications, and to keep trying.
"But if they're not able to reach them, they can contact their local Red Cross Chapter to begin a family tracing service," she said.
In Jersey City, which has one of the largest Filipino communities in the nation, Mayor Steven Fulop and City Council President Rolando Lavarro have announced a fundraiser will be held to aid the victims of the Typhoon from 6 p.m. to 9 p.m. on Thursday, November 14th at Porto Lounge, 286 First Street, in Jersey City.
A representative from the American Red Cross will be on hand to collect checks for the relief effort, and Porto Lounge will donate a portion of their receipts as well.
"We know that our Filipino residents are all extremely concerned about their loved ones who are in the Philippines," said Mayor Fulop. "As the relief effort is going to take much time and billions of dollars, we are asking residents in Jersey City to help aid in the mission."


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If you've seen any bank commercials lately, you'd think mobile banking is a standard feature on bank accounts these days. But while it's gaining in popularity, it's still not a given that your bank offers it.
There are now around 7,000 financial institutions offering mobile banking to retail banking customers, according to an estimate in a new report by Aite Group. Considering there are about a little more than 14,000 banks, thrifts and credit unions in the country, according to data from the Federal Deposit Insurance Corp. and the National Credit Union Administration, that means about half of all financial institutions offer customers the ability to bank on their cellphones.
But, to get the most out of mobile banking, you need a smartphone. Just 37 percent of U.S. consumers have one compared to 43 percent still rocking a less advanced, less expensive "feature phone," according to the report.
Considering the limited tech offerings available on feature phones, it's no surprise that fewer than 10 percent of feature-phone users do something as simple as check their bank balances from their phones, according to the Aite report.
Even if you do have a smartphone, you're more likely than not to skip mobile banking. Just 36 percent of smartphone users say they use their phone to check their account balances, according to the Aite report.
We may see those numbers grow quickly. The reports' authors expect smartphone ownership to rise by about two-thirds over the next four years. But, it's worth remembering that this is a technology still being used by a relatively small number of bank customers. Most people still prefer banking via PC or in person, which is why a significant percentage of banks are still holding back on offering mobile banking.
I think that's a shame. Mobile banking is one of the quickest and most convenient ways to check on your money in real time. If you happen to be shopping for a bank and are also a fan of mobile banking, it might worth asking about a bank's tech features before signing on the dotted line.
What do you think? Is not offering up-to-date technology, including mobile banking, a deal breaker for you? Do you use mobile banking?
Follow me on Twitter: @claesbell.


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British, French leaders meet for defence summit
British Prime Minister David Cameron hosts French President Francois Hollande at their first bilateral summit on Friday, likely to be dominated by defence issues but also tensions over the EU.
The first Anglo-French summit since Hollande's election in 2012 takes place at a military base in Cameron's constituency in Oxfordshire, west of London, and will be followed by lunch at a country pub.
Away from the matters of state, Hollande is also unlikely to escape questions at a press conference about his recent split with his partner, Valerie Trierweiler.
The setting reflects the strong defence theme. The leaders are expected to sign memoranda of understanding to cooperate on an anti-ship missile, an unmanned combat air vehicle and a submarine mine detector.
Cameron and Hollande, accompanied by their defence and foreign ministers, will also discuss a 10,000-strong joint combat force proposed to be operational by 2016.
The talks build on a landmark agreement on defence and security cooperation signed in London in 2010 by Cameron and Hollande's predecessor, Nicolas Sarkozy.
British-French deals on nuclear energy are also on the programme, as are discussions on Syria and Iran, on which the two leaders are closely aligned.
Cameron is also likely to use the half-day summit at the Royal Air Force (RAF) base at Brize Norton to press a more contentious subject -- European Union reform.
Under pressure from eurosceptics in his Conservative party, Cameron has promised to renegotiate Britain's membership of the EU and put the new deal to a referendum by 2017, provided he wins the 2015 election.
But French officials warned this week that while some EU treaty changes may be necessary, it was not at all clear they would go through in the timeframe Cameron requires.
One official complained that the referendum pledge had opened up a "permanent debate" in Britain about its role in Europe that had only hardened the eurosceptic position.
In that context "accidents are possible", the official said, referring to the danger that British voters opt to quit the EU.
"We have a shared interest that Britain stays in Europe, but not at the price of dismantling the EU," the official added.
At the back of Hollande's mind in the pub on Friday will also be the frequent swipes made by Cameron's Conservatives about the Socialist president's handling of the French economy.
Even if such criticisms are "neither justified or agreeable", France will rise above it, an aide said, adding there would be no reciprocal "British-bashing".


text: This is my list of some players that might be available in the 2nd or 3rd rounds. I seems that trading down in the 1st to get and extra 2nd or 3rd could be really valuable this year. 3-4 OLB Anthony Barr Ezekiel Ansah Cornellius Carradine Brandon Jenkins Corey Lemonier Michael Buchanan Chase Thomas Dline Sharrif Floyd Sylvester Williams Safety Kenny Vaccaro Matt Elam Eric Reid Tony Jefferson Shawn Williams Bacarri Rambo D.J. Swearinger Robert Lester WR Cordarrelle Patterson Tavon Austin OL - Interior Khaled Holmes Barrett Jones Larry Warford Gabe Jackson Alvin Bailey OT and OT/OG Jake Matthews Eric Fisher Dallas Thomas Lane Johnson Oday Aboushi Brian Winters Brennan Williams Kyle Long


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NEW CANEY, Texas - Four people were arrested in New Caney on Wednesday after investigators said they discovered the group was involved in a family drug business.
Sheri Ann Young, 51, was arrested in a travel trailer in which she's accused of running a meth operation. Her son, Michael J. Young, 29, was arrested with Moriah Joy Carroll, 20, and Robert Reneau, 43, during a traffic stop.
Montgomery County Precinct 4 deputy constables said the arrests were made after they investigated claims that Sheri Young was selling methamphetamine from a trailer on a property in the 21600 block of Punkin.
According to investigators, surveillance of the residence showed a large number of visitors who stayed for short periods of time, which raised suspicions.
Around 1:20 p.m. on Wednesday, a Precinct 4 lieutenant conducted a traffic stop on a red Chevy S-10 pickup, which was one of the vehicles that was used during a short visit at Young's residence.
Two men and two women were traveling eastbound on McCleskey in the truck. One of the women was sitting on a man's lap and neither had a buckled seatbelt, deputy constables said.
As the deputy approached and activated his emergency equipment, he said he could see the woman on the male's lap lean over, out of site, for several seconds. The deputy said that led him to suspect she was attempting to hide contraband, investigators said.
The lieutenant said he recognized the driver, Reneau, who acted extremely nervous and was visibly shaking.
A search of the vehicle and its contents revealed 14 pills identified as a dangerous drug in Carroll's purse, deputies said.
The lieutenant said he also discovered a white plastic container with a glass pipe and over a half gram of methamphetamine. Reneau was detained and his passengers were then identified as Michael Young, Carroll, and a second woman who was the only member of the group that did not end up in jail.
Deputies said Carroll initially denied having any contraband with her, but later admitted that she hid methamphetamine in her vaginal area.
According to Carroll, Michael Young, whose lap she was sitting on at the time of the stop, tried to force the drugs down her pants when he saw law enforcement, deputies said. Authorities said Carroll agreed to hide the drugs.
Meanwhile, investigators conducting surveillance on Sheri Young's residence said they saw her emerge from the camper trailer carrying a wicker basket, which she placed behind the camper.
After deputies took the suspects to the Precinct 4 office for booking, the lieutenant paid Sheri Young a visit. The lieutenant said the wicker basket contained glass smoking pipes with methamphetamine residue, two digital scales and a marijuana smoking pipe.
Deputies said a search of the residence revealed another large digital scale, an empty box for a different digital scale and multiple used syringes containing a red liquid.
Multiple small plastic bags typical of those used to package narcotics for sale were found inside Sheri Young's purse, investigators said.
Sheri Young was taken into custody and transported to the Precinct 4 office for booking. While in the office, she collapsed and began having a seizure that lasted more than 10 minutes, deputies said.
Authorities said an ambulance responded and transported the mother to the emergency room in Kingwood, where hospital staff removed a clear plastic bag containing over three grams of methamphetamine from her vaginal cavity. She was later released and the booking process continued.
Sheri Young is charged with third-degree felony possession of a controlled substance.
Michael Young was charged with third-degree felony possession of a controlled substance and third-degree felony for tampering/fabricating physical evidence with intent to impair.
Carroll was charged with third-degree felony possession of a controlled substance and third-degree felony for tampering/fabricating physical evidence with intent to impair and class-A misdemeanor possession of a dangerous drug.
Reneau has been charged with state jail felony possession of a controlled substance.
Copyright 2013 by Click2Houston.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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A 20-YEAR-OLD man stole money from a bedside cabinet as his seriously ill mum slept in her hospital bed.
Blackburn magistrates heard Craig Malcolm Keary was spotted by a nurse as he stole £800, which was his mum's disability living allowance that had built up during her time in hospital.
A police dog later found the purse but the money was missing and had not been recovered.
Keary, of Swift Close, Blackburn, pleaded guilty to theft of £800 belonging to Louise Murphy. He was remanded on bail until August 21 for the preparation of a pre-sentence report.
Catherine Allan, prosecuting, said when the offence took place Mrs Murphy had been in hospital for five weeks.
"She was asleep on the ward when a nurse saw the defendant come in and remove a purse from the bedside cabinet," said Miss Allan.
Peter King, defending, said the offence had been committed in November and since then Mrs Murphy had been discharged from hospital.
"She has forgiven her son," said Mr King. "They spoke on New Year's Eve and she said she would endeavour to withdraw her complaint."
Mr King said Keary had wanted money for a deposit on his own accommodation.
"Immediately after the incident he regretted what he had done and has regretted it ever since," said Mr King.
"He accepts his mum was vulnerable in the circumstances."


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With flexible, slip resistant outsoles, our ICE Collection contains boots built for tough conditions and long-lasting wearability.Outstanding abrasion, Oil and Slip Resistant. Old Town Folklore leather vamp and perforated saddle.Golden beige rangedocker foxing, leather-lined quarter and Perfed Perma-fresh "footprint" insole. "Oak Ice" oil and slip resisting outsole, a stacked heel, and red soft touch shaft with 8 row stitching. Made in the USA.


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Gold Initial Necklace with Four Discs
A delicate gold initial disc necklace by Chupi
Show the world the initial that means the most to you; is it your own, or the one you love? Or maybe both? From a single initial as a treat for you, to a new baby's initial and their birthstone to match, or three initial discs for three children's names we have made some beautiful bespoke necklaces. We have A-Z, the & symbol and a selection of gemstones to choose from. Whether you choose one initial or five this necklace will truly belong to you.
Choose your favourite initial and we will make a necklace especially for you.
-The chain is 18 inches/ 45cm long
-14k gold filled chain
-9mm round 14k gold filled disc hand-stamped with the letter of your choice
-Finished with a 14k gold filled lobster clasp and a Chupi charm
Each disc is hand stamped using an old fashioned cast iron stamp. Every letter is different because we love the individuality of hand stamping.
Individually handmade in our studio in Dublin, Ireland.
This piece is exclusively available on Chupi.com & in our store in Powerscourt Townhouse, Dublin 2, Ireland.


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|Home > Insurance Division > Individual Health Coverage Program > Meeting Schedule
|IHC Program Meeting Schedule
| Ellen DeRosa,
Executive Director
New Jersey Individual Health Coverage Program
P.O. Box 325, 20 West State Street, 11th Floor
Trenton, NJ 08625-0325
|Telephone: 609-633-1882 ext. 50302
The New Jersey Individual Health Coverage ("IHC") Program Board has established its 2016 meeting schedule which is set forth at right.
Interested parties may call the IHC Program at 609-633-1882 ext. 50302 or access the Department of Banking and Insurance web site at www.dobi.nj.gov/ihc/ to check on any meeting dates.
|OPRA is a state law that was enacted to give the public greater access to government records maintained by public agencies in New Jersey.
|You will need to download the latest version of Adobe Acrobat Reader in order to correctly view and print PDF (Portable Document Format) files from this web site.
| Copyright © 2011,
State of New Jersey
New Jersey Department of Banking and Insurance


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Toys that provide positive reinforcement can greatly benefit autistic children.
When it comes to effective behavior management, many doctors believe that positive reinforcement is one of the best methods for dealing with the challenging behaviors of children with autism or Asperger's Syndrome. These difficult conditions can not only make it complicated for children to learn new behaviors or important life skills, but can even make it challenging for them to express their difficulty with these actions.
Positive reinforcement, however, can help encourage positive behaviors and reward the action so that children are likely to repeat the same action or behavior in the future. For instance, if a child engages in a certain behavior or action and they are rewarded with something that they wanted, they are more likely to repeat it again in the future―especially if they are met with this same reward again.
However, the term "positive" in positive reinforcement does not necessarily mean something "good" as a reward. Instead, it simple relates to the more mathematical term of "adding" or "addition." i.e. One action directly an additional action. Without one, there would be no additional action. Therefore, positive reinforcement does not necessarily have to come in the form of a reward or a bribe.
In fact, there are many toys and activities that were created with autistic children in mind as a way to provide positive reinforcement for their play experiences. For instance, some toys may make noise or light up when a child gets an answer correct. Others may produce an effect when a puzzle piece is correctly inserted in the right way. No matter what the action is that produces the positive reinforcement, it has been proven that many kids with developmental disorders feed on this type of praise and are more likely to repeat their action afterwards.
Laser Pegs is one type of positive reinforcement toy that has been shown to have benefits for children with autism. Here are just a few reasons why these toys are so helpful for children with developmental disorders:
Gives immediate positive reinforcement: In order to a child to make a direct connection between their action and the positive reinforcement that they receive, the reward must be given immediately following the desired behavior. For instance, the immediate light-up effect of Laser Pegs allows a child to make an instant connection between their behavior of connecting the toys and the positive reaction of the play structure lighting up.
Promotes Positive Actions
According to a recent article on the relationship between autism and positive reinforcement, a true system of positive reinforcement includes presenting tasks and lessons which have been broken down into simple steps, allowing the child to experience small incremental successes while working toward a larger goal. Laser Pegs is a great example of how a child can see block by block how their actions turn into rewards.
Develop Better Relationships
Laser Pegs and similar toys can also help to build a child's relationship with their parents. This is because parents are able to change their response and reaction based on how their child is engaging with the toy, both positively and negatively. Parents can also show their children how to correctly connect the Laser Pegs block in order to produce the lighted effect, and help to manage any frustration or difficulty their child may be having by working one on one with them.


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Shrimp Scampi:
20 ea Medium Shrimp
3/4 # Linguine Pasta
1 Tbsp Fresh Minced Garlic
7 Tbsp Butter
1/4 C Heavy Cream
1/2 C White Wine
1 tsp Parsley
Add 1 Tablespoon of the butter to a Medium high heat large non-stick saute pan. When butter has melted add the shrimp to the pan and saute until half cooked. Add the garlic and whtie wine and reduce until syrupy, then add the cream and reduce again until thickened. Add the cooked pasta to the pan and then top with the remaining butter. Turn off the heat and mix all ingredients in the pan to incorporate thoroughly. If the sauce seem to break add just a touch of hot water and stir all ingredients again to bring the sauce back together. Serve in appropriate side dish and garnish.
Sherry Cream Sauce:
1 QT Heavy Cream
1/8 C Worcestershire
1/4 T Dry Mustard
3/4 C Sherry
1/4 C Brandy
1/8 C Brown Sugar
3/4 T Kosher Salt
1/5 C Blonde Roux
1/4 tsp White Pepper
Copyright 2012 by News4Jax.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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A TEENAGE first aider working for St John's Ambulance has been nominated for an award.
Adam Evans, right, of Hambridge, a 17-year-old cadet with the charity's Taunton unit, has been made the South-West's representative for the service's annual Volunteer of the Year award.
It could see him meet TV's Tess Daly, who will compere the star-studded Everyday Heroes awards bash on March 26.
Adam, who has volunteered with St John since the age of five, is up against seven regional finalists and is hoping for enough votes from St John's volunteers in an online poll until Sunday, March 2.
Adam said: "I'm astounded and humbled at being chosen to represent the South-West.
"I knew I did a lot, but you always get the feeling other people do more, so it's a real privilege to be noticed."
For information on the awards, click here.


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Used 2013 Jeep Values
Read more Read less
Jeep 2013 Lineup: Jeep has grown its best-selling brand from within its own ranks, choosing to focus on two of its best-selling and flagship vehicles for 2013. The Grand Cherokee gets a 241-horsepower turbo-diesel V6 engine with major updates to the Overland Summit models that add more power and luxurious features and amenities than ever before.
The Wrangler, Jeep's other flagship auto, gets new seats, an improved soft top that is more easily opened and an optional insulated soft top. Compass and Patriot both receive subtle trim updates, but are otherwise unchanged, as is the Liberty.
Select a 2013 Jeep model below to get used trade-in values and retail prices
(2018 - 1930)
2013 Jeep Compass
A perfect blend of off-road capabilities and convenient daily driving features, the 2013 Jeep Compass offers driver the best of both worlds.
2013 Jeep Grand Cherokee
Boasting a towing capacity of 7,000 lbs. and superior off-road capabilities, the 2013 Jeep Grand Cherokee is an ideal sport utility vehicle.
2013 Jeep Patriot
Available to be outfitted in an assortment of variations, the 2013 Jeep Patriot can be equipped with 4WD, a 2.-liter 4-cylinder engine, and CVT transmission.
2013 Jeep Wrangler
Perhaps one of the best known off-road vehicles, the 2013 Jeep Wrangler carries on the spirit of adventure with a modern twist.
2013 Jeep Wrangler Unlimited
Equipped with removable doors, the 2013 Jeep Wrangler Unlimited offers all the utility of the original Wrangler fused with the convenience of four doors.


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Media streaming has become a staple of our daily digital consumption. So much so that this week music lovers in the US finally welcomed the arrival of Europe's music-streaming subscription service Spotify, while angry Netflix customers turned to social networking sites to vent their rage over subscription fee increases. Read more
© The Financial Times Ltd 2016 FT and 'Financial Times' are trademarks of The Financial Times Ltd.


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Gattis, J-Up back Medlen's seven strong
MIAMI -- Much of the buzz surrounding this year's Braves has been centered around Justin Upton's red-hot start and the power that exists throughout their lineup. But the team's early-season success has been significantly influenced by its overlooked pitching staff.
As the offense remained relatively quiet on Tuesday night at Marlins Park, Kris Medlen regained the dominant form he displayed last year and benefited from the two-run home run Evan Gattis supplied the Braves in their 3-2 win over the Marlins.
"What else can you say about Evan Gattis?" Medlen said. "He was the difference in the game for me."
With Freddie Freeman on the disabled list, Gattis has been moved into the cleanup spot in just the second week of his Major League career. The 26-year-old rookie catcher displayed his power potential to Marlins starter Wade LeBlanc with his first-inning home run that bounced and hit the large artistic display beyond the left-center field wall.
Gattis' second career homer provided some early cushion for Medlen, who limited the Marlins to one unearned run over seven innings. But Upton once again provided the crushing blow despite going homerless for just the third time this season.
Andrelton Simmons opened the eighth inning with an infield single and advanced to second base on right-handed reliever A.J. Ramos' throwing error. He scored easily when Upton's double dented the center-field wall.
"Any time you have a chance to add on, it's big because it kind of eliminates some stuff from the opponent," Braves manager Fredi Gonzalez said. "That was a big run that Justin drove in."
Upton's double provided the Braves with a necessary insurance run, considering Eric O'Flaherty allowed the Marlins to score an eighth-inning run on Placido Polanco's two-out single. O'Flaherty issued a leadoff walk to Austin Kearns and then intentionally walked the dangerous Giancarlo Stanton to put runners on the corners with two outs.
Polanco followed with his single before O'Flaherty ended the inning without further damage. Braves closer Craig Kimbrel kept things interesting by walking Donovan Solano to begin the bottom of the ninth. But Ramiro Pena, who entered to replace the less-mobile Juan Francisco at third base in the eighth inning, quieted the threat with a clutch double play. Pena raced toward the plate to catch Rob Brantly's bunt in the air and then fired to first base to retire Solano, who was unable to get back to the bag in time.
"He got us out of that jam," Gonzalez said. "If not, we might still be playing that game, or I might be sitting out there losing more hair."
While winning seven of their first eight games, the Braves have provided the bald Gonzalez plenty of reasons to be jovial. They have won five straight and allowed just two earned runs in their past three games. Their starting pitchers have limited the Marlins to four hits in the first two games of this series.
"If we keep doing that, we're going to be OK," said Gonzalez , whose pitchers have compiled a 2.13 ERA. "The offense will come around eventually. We've got too many guys who are too good of hitters for it not to come around. But I'm really pleased with the way we've been pitching."
Following the lead of Paul Maholm, who allowed one hit in seven scoreless innings on Monday, Medlen scattered three hits and issued two walks in his seven-inning effort. The only run he surrendered came after B.J. Upton dropped Greg Dobb's fly ball in deep center field to begin the bottom of the second inning.
Dobbs advanced to third base on Solano's infield single and scored on Brantly's sacrifice fly.
While Medlen's defense hurt him in the second inning, it saved him after Polanco began the bottom of the fourth with a double. Playing in on the grass, Simmons made a diving grab of Solano's hard grounder and prevented Polanco from scoring.
Medlen retired the final 10 batters he faced and totaled just 24 pitches in his final three innings. In the process, he distanced himself from the mechanical flaws that plagued him during Spring Training, and he looked more like he had while posting a 0.97 ERA in the 12 starts he made after moving from the bullpen to the rotation last year.
"Once I felt my delivery kick in, I just felt comfortable," Medlen said. I knew if I made the pitches I needed to make, I'd have quicker at-bats. The last three innings I threw, I had single-digit pitch counts each inning. That felt like the old me."
Mark Bowman is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.