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Ephrin-A1 inhibits NSCLC tumor growth via induction of
Tumor formation is a complex process which involves constitutive activation of oncogenes and suppression of tumor suppressor genes. Receptor EphA2 and its ligand ephrin-A1 form an important cell communication system with its functional role in cell-cell interaction and tumor growth. Loss of cell-cell adhesion is central to the cellular transformation and acquisition of metastatic potential. Claudins, the integrated tight junction (TJ) cell-cell adhesion proteins located on the apico-lateral portion of epithelial cells, functions in maintaining cell polarity. There is extensive evidence implicating Eph receptors and ephrins in malignancy, but the mechanisms how these molecular players affect TJ proteins and regulate tumor growth are not clear. In the present study we hypothesized that EphA2 signaling modulates claudin-2 gene expression via induction of
The expression of EphA2, claudin-2 was determined in various NSCLC cell lines by using real-time quantitative polymerase chain reaction and Western blot analysis. The claudin-2 expression was also analyzed by immunofluorescence analysis. EphA2 and
NSCLC cells over expressed receptor EphA2 and claudin-2. Ephrin-A1 treatment significantly down regulated the claudin-2 and EphA2 expression in NSCLC cells. The transient transfection of cells with vector containing ephrin-A1 construct (pcDNA-EFNA1) decreased the expression of claudin-2, EphA2 when compared to empty vector. In addition ephrin-A1 activation increased c
Our study suggests that EphA2 signaling up-regulates the expression of the TJ-protein claudin-2 that plays an important role in promoting cell proliferation and tumor growth in NSCLC cells. We conclude that receptor EphA2 activation by ephrin-A1 induces tumor suppressor gene
Tight junctions (TJ), the most apical cell-cell adhesion, owing to their cellular location are responsible for maintaining the cellular integrity. Any deregulation of the TJ characteristics could potentially lead to cellular transformation and acquisition of tumorogenesis potential
The Eph family of receptor tyrosine kinases plays key role in the development of cancer. The Eph receptors and ephrins were originally discovered as neuronal guidance and vasculature formation proteins during embryonic development
NSCLC cell culture
A549 NSCLC cell line was obtained from American Type Culture Collection (Manassas, VA) and NCI-H2126, NCI-H838, NCI-H522, NCI-H23 NSCLC cell-lines were a kind gift from Dr. Frederic Kaye, MD, Division of Haemato-logy/Oncology, University of Florida, Gainesville, Florida. The NSCLC cells were resuspended in RPMI-1640 (Gibco Laboratories, Grand Island, NY) containing 10% FBS, penicillin (100 U/ml) and streptomycin (100 μg/ml). The cells were plated in 100 mm Petri dishes (Corning Costar Corporation, MA) and incubated at 37°C in 5% CO2 and 95% air. The cell culture medium was changed on alternate days. When the cells were confluent they were trypsinized and seeded into 100 mm culture dishes or transwell chambers as required for different assays.
Construction of vectors containing ephrin-A1 (EFN-A1) and EphA2 and transient transfection of NSCLC cells
The gene transfer vector, pcDNA3.2/V5-DEST was used as an expression vector for the expression of ephrin-A1 (EFN-A1), and receptor EphA2, and pcDNA3.2/V5/CAT was used as a control vector (Invitrogen, Carlsbad, CA) as reported earlier
Transfection of NSCLC cells
The siRNA targeting the receptor EphA2 and
Total RNA isolation and quantitative real time PCR analysis
Total RNA from cultured NSCLC cells was isolated and diluted with RNase-free water to 100 ng/mL; then, 10 μl of each sample were reverse transcribed into complementary DNA (cDNA) as reported earlier
Western blot analysis
NSCLC cells were cultured in 60 mm size cell culture dishes (Fisher Scientific, Pittsburgh, VA) to confluence and the cells were lysed in lysis buffer with the method reported earlier
NSCLC cell proliferation
NSCLC cell proliferation was assessed by using an assay based on cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases (Roche, Indianapolis, IN) as reported earlier
Immunofluorescence microscopy
Claudin-2 expression in NSCLC cells was analyzed by using confocal laser-scanning microscopy (Zeiss LSM 510, Axiovert 100 M; Zeiss, Thornwood, NY), as reported previously
NSCLC tumor growth in matrigel
A 48-well culture plate was coated with 200 μl of matrigel per well and then allowed to polymerize for about 1–2 hours at 37°C. NSCLC cells at a density of ~1 x 103 cells per well were plated in 0.3 ml of 2% FBS containing RPMI-1640 as reported earlier
Statistical analysis
The Sigma Stat 3.5 statistical software program was used to calculate statistical significance. Kruskal-Wallis One Way Analysis of Variance (ANOVA) was used to compare the experimental groups from the control groups. The post hoc test Holm-Sidak method was applied for pairwise comparisons. The differences at
NSCLC cells express receptor EphA2 and Caludin-2
Proliferating NSCLC cells (A549, H2126, H838, H522, and H23) expressed receptor EphA2, and claudin-2 and the expression of ephrinA1 was variable (Figure
Figure 1
NSCLC cells express receptor EphA2, Ephrin-A1 and Caludin-2
NSCLC cells express receptor EphA2, Ephrin-A1 and Caludin-2. Plate A: NSCLC cell lines (A549, H2126, H838, H522, H23) express receptor EphA2, ephrin-A1, and claudin-2. Expression of EphA2, ephrin-A1, and claudin-2 was analyzed by Western blot analysis. Plate B: Western blot analysis of EphA2 protein expression the β-actin was probed to demonstrate equal sample loading. NSCLC cell lines were either transfected with pcDNA-EFNA1 (pcDNA-EFNA1 is vector containing ephrin-A1 construct) or empty vector and receptor EphA2 expression was analyzed. Plate C: A549 cells were activated for 5 minutes to 120 minutes with ephrin-A1. Western blot analysis of phosphorylated EphA2 and
Ephrin-A1 down regulated claudin-2 expression in NSCLC cells
Ephrin-A1 activation of receptor EphA2 down regulated the expression of claudin-2 in NSCLC cells. To identify whether decrease in claudin-2 expression is directly due to decrease in EphA2 receptor expression, A549 cells were transfected with pcDNA-EFNA1 or empty vector or left untransfected. In comparison to empty vector the transient transfection of A549 cells with pcDNA-EFNA1 resulted in significantly decreased claudin-2 expression (Figure
Figure 2
Ephrin-A1 activation decreases claudin-2 expression in NSCLC cells
Ephrin-A1 activation decreases claudin-2 expression in NSCLC cells. Relative expression of claudin-2 mRNA normalized to endogenous control. Plate A: Cells were either activated with ephrin-A1 or transfected with pcDNA-EFNA1 (vector containing Ephrin-A1 construct) or empty vector. Plate B: Cells either silenced with EphA2 siRNA or transfected with pcDNA-EphA2 (pcDNA-EphA2 is vector containing EphA2 construct) Plate C: Claudin-2 protein expression by Western blot analysis, β-actin was probed to demonstrate equal sample loading. Plate D: Western blot analysis of EphA2 protein expression the β-actin was probed to demonstrate equal sample loading. Data presented was the mean ± SEM of three independent experiments,
In order to determine if over expression of the receptor EphA2 in A549 cells promotes claudin-2 expression, A549 cells were transiently transfected with pcDNA-EphA2 and the expression of claudin-2 was evaluated. A significant increase in the expression of claudin-2 was noted when compared to empty vector transfected cells (Figure
Claudin-2 expression decreased in Ephrin-A1 treated A549 cells
In order to evaluate the cellular distribution of caludin-2, and morphological changes in activated cells, A549 cells were activated with ephrin-A1 and analyzed by immunofluorescence microscopy. Fluorescence immuno-staining analysis revealed that A549 cells showed punctuated expression of claudin-2 whereas, ephrin-A1 activation decreased claudin-2 expression (Figure
Ephrin-A1 treatment increased
Figure 3
Transfection of pcDNA-EFNA1 increased whereas transfection of pcDNA-EphA2 attenuated
Transfection of pcDNA-EFNA1 increased whereas transfection of pcDNA-EphA2 attenuated
Activation of A549 cells with ephrin-A1 or transfection with pcDNA-EFNA1 resulted in significant decrease of claudin-2 expression. In addition over expression of
Figure 4
Ephrin-A1 activation and transfection with ephrin-A1 vector pcDNA-EFNA1 resulted in suppression of proliferation in A549 cells when compared to control or empty vector transfected cell (Figure
Figure 5
Ephrin-A1 activation attenuates NSCLC cell proliferation
Ephrin-A1 activation attenuates NSCLC cell proliferation. Proliferation of NSCLC cells as measured by WST-1 assay. Plate A: Proliferation in cells either activated with ephrin-A1 or transfected with pcDNA-EFNA1 (pcDNA-EFNA1 is vector containing ephrin-A1 construct). Plate B: Proliferation in cells either silenced with EphA2 siRNA or transfected with pcDNA-EphA2 (pcDNA-EphA2 is vector containing EphA2 construct). Plate C: Proliferation in
Ephrin-A1 activation inhibited tumor growth and silencing
A549 cells were activated with eprhin-A1 or transfected with a plasmid pcDNA-EFNA1, or empty vector, or left untransfected as control. The transfected or control cells were plated on 3-D matrigels to determine the tumor growth
Figure 6
Ephrin-A1 ligand activation decreases tumor growth in NSCLC
Ephrin-A1 ligand activation decreases tumor growth in NSCLC. Equal number of A549 cells was seeded in matrigels after various treatments as described and after 7 days of culture tumor growth was recorded by a SPOT digital camera attached to Nikon microscope. The data presented are a single representative of three similar but independent experiments. Magnification = 5 μM.
The major finding of our present study is that receptor EphA2 is over expressed in NSCLC cell lines which promotes tumor growth. In addition we also found that EphA2 promotes tight junction protein claudin-2 expression in A549 cells. However, the expression of ephrin-A1 was found to dysregulated and A549 cells showed minimal levels. There is accumulating evidence that activation of receptor EphA2 with its ligand ephrin-A1 attenuates tumorogenic potential of malignant cells
EphA2 is an oncoprotein which promotes cell survival, abnormal cell growth and invasion in a number of malignancies, including NSCLC
We demonstrate that activation of receptor EphA2 with ephrin-A1 induced
It has been shown that, caudal-related homeobox gene cdx-2 is positively involved in the regulation of the human claudin-2 promoter activity
In conclusion, we present the first evidence that EphA2 signaling promotes the expression of claudin-2 in NSCLC cells. Activation of NSCLC with ligand ephrin-A1 suppressed the caludin-2 expression via the induction of transcriptional factor
Competing interests
The authors have no competing interests.
Authors' contributions
BSG, carried out the experiments. NN and BSG drafted the manuscript. NN and KAM critically analyzed, edited, reviewed and finalized the data. FK, for providing cell lines and editorial review, EPG provided the valuable comments and editorial review of the manuscript. All authors' read and approved the final manuscript.
This work was supported by NIR grant # 09KN-09; RC1 (#09KW-08) from James & Esther King, Florida Department of Health (Nasreen, N); VA Merit Review (Mohammed, KA).
Claudin family of proteins and cancer: an overview
Regulation of tumor angiogenesis by integrin-linked kinase (ILK)
Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast
Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus
Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease
Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer
Tight junction proteins claudin-3 and claudin-4 are frequently overexpressed in ovarian cancer but not in ovarian cystadenomas
Expression of claudins 1, 2, 3, 4, 5 and 7 in various types of tumours
Increased expressions of claudin-1 and claudin-7 during the progression of cervical neoplasia
Claudin-3 and claudin-4 expression in ovarian epithelial cells enhances invasion and is associated with increased matrix metalloproteinase-2 activity
Claudin-1 is a metastasis suppressor and correlates with clinical outcome in lung adenocarcinoma
Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins
Changing intestinal connective tissue interactions alters homeobox gene expression in epithelial cells
Expression of the Cdx1 and Cdx2 homeotic genes leads to reduced malignancy in colon cancer-derived cells
Cdx-2 homeodomain protein expression in human and rat colorectal adenoma and carcinoma
Molecular cloning, sequencing and expression of the mRNA encoding human Cdx1 and Cdx2 homeobox. Down-regulation of Cdx1 and Cdx2 mRNA expression during colorectal carcinogenesis
Cloning of the human claudin-2 5'-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1alpha
Predictive value of the EphA2 receptor tyrosine kinase in lung cancer recurrence and survival
ECK, a human EPH-related gene, maps to 1p36.1, a common region of alteration in human cancers
Overexpression of the EphA2 tyrosine kinase in prostate cancer
EPHA2/EFNA1 expression in human gastric cancer
Silencing the receptor EphA2 suppresses the growth and haptotaxis of malignant mesothelioma cells
EphrinA1 inhibits malignant mesothelioma tumor growth via let-7 microRNA-mediated repression of the RAS oncogene
Receptor EphA2 activation with ephrinA1 suppresses growth of malignant mesothelioma (MM)
Silencing receptor EphA2 induces apoptosis and attenuates tumor growth in malignant mesothelioma
Pleural mesothelial cell (PMC) defense mechanisms against malignancy
Ligation of EphA2 by Ephrin A1-Fc inhibits pancreatic adenocarcinoma cellular invasiveness
Soluble Eph A receptors inhibit tumor angiogenesis and progression in vivo
Human malignant mesothelioma cell lines express PDGF beta-receptors whereas cultured normal mesothelial cells express predominantly PDGF alpha-receptors
Overexpression and functional alterations of the EphA2 tyrosine kinase in cancer
Claudin proteins in human cancer: promising new targets for diagnosis and therapy
Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells
Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells
Cdx2 regulates endo-lysosomal function and epithelial cell polarity
CDX-2 homeobox gene expression in human gastric carcinoma and precursor lesions
Expression of Cdx-2 homeobox gene in intestinal metaplasia and gastric adenocarcinoma
Altered expression of CDX-2, PDX-1 and mucin core proteins in "Ulcer-associated cell lineage (UACL)" in Crohn's disease
CDX-2 expression in pulmonary fine-needle aspiration specimens: a useful adjunct for the diagnosis of metastatic colorectal adenocarcinoma
CDX-1 and CDX-2 are expressed in human colonic mucosa and are down-regulated in patients with Hirschsprung's disease associated enterocolitis
Downregulation of the colon tumour-suppressor homeobox gene Cdx-2 by oncogenic ras
Pre-publication history
The pre-publication history for this paper can be accessed here:


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|Specific References
|Related Products
|Human Cells
|Human Cell lysate that Human CD50 / ICAM3 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
|The extracellular domain (Met 1-His 485) of human ICAM3 (NP_002153.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
|Gln 30
|The recombinant human ICAM3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 703 amino acids and predicts a molecular mass of 77.2 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3/Fc is approximately 125-135 kDa due to the glycosylation.
|Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
|Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
|12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
|Samples are stable for up to twelve months from date of receipt.
|1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
|1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
|Store at 4℃. After re-dissolution, aliquot and store at -80℃.
|Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

subset: commoncrawl


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Forrest Wheeler
Fresh Off the Boat
Eddie Huang and his Taiwanese family are on the move from D.C. to Orlando so his father can open a rustic steakhouse. While Dad wants his "American Dream," Mom suffers culture shock in this new city. Eddie and his brothers just want to fit in.


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After a hard day of work or an eight hour power nap, chances are you will awaken feeling as slow and sluggish as a sloth. Etsy seller appendageaccessories can help you illustrate this point with a canvas sloth sleep mask that comes equipped with breathable foam lining and a 3/8″ thick adjustable elastic strap. The hand drawn image also provides a pleasing, happy appearance that can help counter the under eye bags and scowls that accompany sleepiness.
If sloths aren't your thing (how could they not?), you can choose from a number of different designs, including several pictured after the break.
Product Page: ($20)


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New, Pretty Little Liars, 'A' Related Scoop From Ashley Benson10/04/2012
Pretty Little Liars: "A" related scoop and more on the Halloween episode, episode 3×13 "This Is a Dark Ride"
Here's some scoop on Pretty Little Liars from Kristin Dos Santos' Spoiler Chat Daily for Thursday 10/4/12.
Miles: Pretty Little Liars scoop, maybe about the epic Halloween episode?
Ashley Benson warns us to fear "the box" in the Halloween episode, calling it "very terrifying." We're not sure what that means, but damn, are we curious! And yes, "A" will be making a visit. That, plus the idea of a terrifying box, makes our anticipation for the PLL Halloween episode shoot through the roof.
Pretty Little Liars returns in 19 days, but not for long. Episode 3×13 of Pretty Little Liars is a stand alone Halloween Special and the show will be back on Mid Season hiatus right after its airing. So soak it up. The good news? All accounts are that its a good episode. Apparently, this season they really wanted to step up the production value and costumes for this special. Ashley Benson even admitted that the first few halloween specials just felt like any other episode, but this one actually is "Special."
Well, this Halloween "Special," airs on Tuesday, October 23, 2012 at 8pm ET on ABC Family. The episode is titled 'This is a Dark Ride,' in reference to the fact that the Halloween party takes place on a moving train. BTW, been at a train party in Europe and they are awesome! Just sayin'.
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- Polished laminate wood with tasteful checkering
- Available in Rosewood and Charcoal Silvertone laminates
- Improves appearance and function
- Secure gripping surface
Pachmayr's Renegade Wood Laminate Pistol Grips are the ultimate in style and function. The wood laminate panels are highly polished and checkered, and available in two colors, Rosewood and Charcoal Silvertone. Both provide classic good looks, and perfectly accent stainless steel and blued frames. The precision CNC cut checking patterns provide a secure grip while maintaining the grip's attractive appearance. Grip screws not included.
Technical Information
Fit: Sig Sauer P938
Material: Wood Laminate


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China's General Administration of Customs issued guidance announcing the launch of a program allowing for consolidated customs duty filing on a nationwide basis. Consolidated customs duty filing is a new process for collecting customs duty in China, and the program is being promoted for its ability to facilitate trades, improve clearance efficiency, and lessen cash flow pressure for qualified enterprises.
The new consolidated customs duty collection program builds on the success of an earlier pilot program. The effective date for this new customs duty collection program is 27 July 2015.
For import and export enterprise satisfying certain conditions, China Customs can calculate and collect taxes on a consolidated basis on the imported and exported goods for a period of time. This method is viewed as providing a benefit to import and export enterprises by improving their trade facilitation and decreasing the costs of customs clearance.
The program's announcement also specifies the conditions for applying for consolidated customs duty collection, the rules for application and operation methods, and disciplinary measures that can be imposed for violations.
Read an August 2015 report prepared by the KPMG member firm in China: Consolidated Duty Collection Is Being Promoted Nationwide
The KPMG logo and name are trademarks of KPMG International. KPMG International is a Swiss cooperative that serves as a coordinating entity for a network of independent member firms. KPMG International provides no audit or other client services. Such services are provided solely by member firms in their respective geographic areas. KPMG International and its member firms are legally distinct and separate entities. They are not and nothing contained herein shall be construed to place these entities in the relationship of parents, subsidiaries, agents, partners, or joint venturers. No member firm has any authority (actual, apparent, implied or otherwise) to obligate or bind KPMG International or any member firm in any manner whatsoever. The information contained in herein is of a general nature and is not intended to address the circumstances of any particular individual or entity. Although we endeavor to provide accurate and timely information, there can be no guarantee that such information is accurate as of the date it is received or that it will continue to be accurate in the future. No one should act on such information without appropriate professional advice after a thorough examination of the particular situation. For more information, contact KPMG's Federal Tax Legislative and Regulatory Services Group at: + 1 202 533 4366, 1801 K Street NW, Washington, DC 20006.
KPMG International has created a state of the art digital platform that enhances your experience, optimized to discover new and related content.


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PowerFlashPoint automatically converts your PowerPoint XP/2002, 2003, 2007, 2010 & 2013 presentations into a highly compressed interactive content in Flash format. Easily distribute and share converted Flash with worldwide audience. It's the worlds most accurate PowerPoint to Flash converter.
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Costco Stock: A Good Long Term Bet
- COSCTO wholesale corporation has a 12 month price target of $152.41
- Increasing membership base and warehouses expansions shows long term growth potential
- The company's performance is majorly driven by net sales, number of warehouses in operations and membership. Sales growth and gross margin is also affected by gasoline price, currency fluctuations and other competition
Costco (NASDAQ: COST) Wholesale Corporation is a Washington based corporation whose subsidiaries operate membership warehouses. It offers members low prices on a wide range of merchandise categories. As of May 10, 2015, Costco operated 673 warehouses worldwide: 474 in United States (in 43 states, Washington, D.C., and Puerto Rico), 89 in Canada, 35 in Mexico, 26 in United Kingdom, 20 in Japan, 11 in Korea, 10 in Taiwan, 7 in Australia and 1 in Spain. The Company's online business operates websites in the U.S., Canada, U.K., and Mexico.
In their recent Q3 earnings report, Costco reported a 1% increase in net sales and 9% growth in net income as compared to the third quarter of 2014. Decrease in gasoline prices and strengthening of the USD negatively impacted the net and comparable sales. Historically, the net sales had been increasing consistently, except for fiscal 2009 (recession). After a 2% drop in revenue in 2009, the sales were back on track.
The company's performance can also be judged by its increasing member base. Increase in membership base implies increase in revenue. Since members pay an annual fee of $55 or $110 to gain access to the discounted products, they majorly tend to buy the products from COSTCO to justify the membership, even if the product is available at other retailers like Walmart! Being a COSTCO member myself, I can totally relate to this tendency.
Membership sign-ups at existing and new warehouses as well as increased penetration of higher-fee Executive Membership program led to 4% YoY increase in membership fees in Q3 2015.
COSTCO has been offering a variety of merchandise at a lower price to all its members. One of the products offered is cars at a reasonable price without any haggling! According to Bloomberg, COSTCO sold almost 400,000 vehicles last year and is at a spitting distance of No. 1 car retailer AutoNation, which sold 533,000 cars last year. Thus, being a member is beneficial not only to get lower prices for various merchandise for daily household use but also travel destinations, hotels, cars, etc. Such value for money deasl makes me believe that COSTCO will keep on attracting old and new members and thereby increasing its member base and revenue.
In the first week of June, the company reported its May sales results. The net sales for the month of May was $8.98 Bn. Barring the impact of gasoline price deflation and currency fluctuation, COSTCO is doing well on comparable sales front. Retail companies report comparable sales (or same store sales) figures as they help assess what portion of sales growth is due to growth in sales from existing stores (open for more than one year). If a retail company is not doing well on comparable store sales front, then it might lead an investor to believe that company sales have reached a saturation point.
The company has been rewarding the shareholders consistently through dividends and its share repurchase program. In April, the company announced a new share repurchase program to the amount of $4 billion which expires on April 17, 2019. Also the quarterly dividend per share has been increased from $0.355 to $0.40 or annual dividend per share of $1.60.
COSTCO's business model of lower price offerings on various merchandise will attract new members and renewals from existing members thereby continuing its growth of member base. With increasing member base and additional upcoming warehouses leading to consistent revenue growth, I expect revenue to grow at an average of 8%. Using the revenue growth expectation and Price to Sales ratio valuation, I arrived at a 12 month price target of $ 152.41. This shows that COSTCO stock has upside potential of nearly 12% as compared to closing price of $ 136.39 on July 2, 2015. I am bullish on COSTCO in the long term.


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- Pierre Hervieux said:
In order to use Gradient Wipe in a Premiere PRO 1.5 widescreen project the image file selected for the wipe must be interpreted correctly with the widescreen pixel aspect ratio (1.422 in your case). Two ways to achieve that:
1- Use an image file where the correct pixel aspect ratio is stored within the file: Photoshop files produced by Photoshop CS and later can do this when the image pixel aspect ratio is set correctly. I believe that older Photoshop files (7 and earlier) cannot do this.
2. The other way is to modify the Interpretation Rules.txt file found in the Plug-ins folder of Premiere PRO (mine is under the "en_US" subfolder, yours will be different) such that Premiere interprets 720x576 files as widescreen with the pixel aspect ratio of 1.422 by default. The file contains instructions on what to change to achieve that.


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Thank you for visiting our website! We are a band of filmmakers, artists and writers in love with making movies. I started Lil Filmmakers in 1999 to offer youth a place to learn about the craft of story telling through the medium of film. I believe art is essential to living a full and balanced life. Art gives us a voice, inspiration and power. Here at Lil Filmmakers Inc. we are advocates for the creative, the nerdy, and the awesomely weird.
Lil Filmmakers- Creative Leaders of Tomorrow
Founder/Executive Director
Lil Filmmakers Inc.'s latest film, Erudition is set to be released in 2017.
A nation devastated by war elects a charismatic but emotionally disturbed president to rebuild the country. But when their new leader bans books and dismantles the country's educational system, its citizens quickly learn that without intellectual freedom they have no freedom at all.
Black Sci-Fi.com Interview with Lil Filmmakers Founder Janine Spruill
Meet our artist in the spotlight- Aria Coleman. -Read more
Watch Jalen's Spoiler Alert of the film AntMan. -Read more
Black Girl Nerds interview with Anissa Cooper and Janine Spruill. -Read more
Update about our movie Erudition from the Writer/Director Anissa Cooper. -Read more


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The Deal
LG LED 1080p 240 Hz Smart 3D HDTVs (manufacturer refurbished). Choose between the following sizes:\
- $849.99 for a 47" TV (47LM8600) ($2,299.99 list price)
- $1,099.99 for a 55" TV (55LM8600) ($2,699.99 list price)
Any price comparison is to a new, nonrefurbished product price.
LG 47" or 55" LED 1080p 240 Hz Smart 3D HDTV (Manufacturer Refurbished)
These LG TV displays pop with a vibrant 1080p high-definition picture. The ultranarrow bezel and nearly frameless cinema-style screen combine to create a sleek look and unobstructed viewing experience. With the included remote, users control their TV by waving a hand, using voice commands, or bribing their new set with a nice foot rub. They can also watch favorite 2D movies in 3D using the TV's conversion technology. The display's Smart TV features allow it to stream music and movies from the Internet and access a wide variety of content from Netflix, Pandora, YouTube, and other providers.
- Full HD 1080p picture quality
- TruMotion 240 Hz refresh rate
- Four HDMI, three USB, and one PC input
- Wide viewing angle
- 2D-to-3D conversion technology
- Ultranarrow bezel and nearly frameless cinema-style screen
- Dual-core processor for faster access to Internet content
- Select features by waving a hand, speaking commands, or using the remote
- Energy Star qualified for being energy efficient and acing its last spelling test
- 90-day warranty from LG
- Condition: manufacturer refurbished
- Click here to view a full list of specifications.
How to get your goods: by purchasing this Groupon and providing your name, shipping address, and phone number your order is complete!
You will be contacted via phone to schedule delivery of your goods, and someone will need to be home to sign upon receipt.
For questions pertaining to this deal, please visit the deal Q&A on this page. For post-purchase inquiries, please contact Groupon customer service.
View the Groupon Goods FAQ for additional information, including how list price is determined.
Like Groupon Goods on Facebook for hand-picked deals, product giveaways, and more Goods stuff.


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When someone asked me what my greatest gaming experience was, I always answered "Metal Gear Solid 3." It's story, characters, graphics, and gameplay were each top-notch. I had nothing to take its place on that list, anyway. Perhaps 999 or Persona 3, but, otherwise, nothing.
Now, going into Silent Hill 2 for the first time, I was worried. I never played a survival-horror game before in my life, due to being scared easily. Hell, the first movie that I was ever scared by traumatized me for life: Alvin and the Chipmunks meat Frankenstein! My little-kid brain could handle it up until the point where the monster walked toward the screen, when I would hide my head in the couch, terrified. I eventually managed to watch the whole thing, but it was still the nail in the coffin when it came to my relationship with horror. Later, I managed to get into Stephen King's work, where I realized, with The Shining and Christine, that I had grown out of the terror I had had at a younger age. I got into horror films shortly afterward, reaching a fandom of The Lost Boys and Nightmare on Elm Street, yet never touched a horror game.
That was where this game came into my life.
Curiosity as to what made these games great was overwhelming as it was, and then GodOfMoogles made a blog series about the series's greatest scares made me try this baby out. Well, actually, I had bought it before, but never got around to it.
Well, only then could I figure it out. Only then could I see the light in this dark and dreary midwestern town. Only then could hopelessness appeal so greatly to me.
Now, allow me to elaborate. Once I popped the disc into my PS2, I realized that, if even the start menu was creepy, then the game as a whole should scare the living crap out of me! Not that my crap is alive, or I eat live things, it's just an expression that I do not know the origins to.
I don't know how we got to the topic of crap, but, you see, this game is quite scary; I'd go so far to call it terrifying, even. While there were individual scened that terrified me, such as a few in the apartment, I really felt the greatest sense of fear in the atmosphere. Of course, atmosphere IS the most imnportant part of horror. I would hardly be afraid of zombies if the game was called "My Little Pony Meets the Carebears in: Lollipop Dead Land."
And so the sense of fear is strongest in the atmosphere, so what? Well, the atmosphere is great in many ways. Fog does a great job of hiding a near-by monster, and that's a part of it, yet, at the same time, it's the weakest link in this game's mighty chain of fear. Seeing blood stains on the ground is another weak-link. The strongest part of the atmosphere is, most definately, the destruction you see in the town. While it's partly just to create a barrier to make you go a long route to your destination, it can also be seen as a major addition to the sense of danger. What created this giant hole in the middle of the road? Well, I don't know, but I don't care; I just want to get the hell out of here!
Another great scare, which is genuinely terrifying, is the game's primary antagonist: Pyramid Head. Throughout the game, you'll see this... thing... stalking you, watching you, raping monsters. Yeah, raping monsters. Don't worry, this much is explained if you think about Pyramid Head's origins. Well, this guy is also your first boss-battle. He's also the only part of the game where you can die if you're playing on easy mode, sadly enough. Hell, beginner combat will let you kill the final boss in three hits with the hunting rifle! Back on topic, this guy is freaky. There's a memorable scene in which he chases you, too, but that's all I'll say about that. Not to mention he has a "great knife" that's bigger than Cloud Strife's sword in Final Fantasy VII!
Of course, this wouldn't be a game if it relied only on scares. In fact, other than the gameplay, which is like you're controlling a drunk unicyclist that just woke up a minute ago, with a missing bolt in his unicycle, it's the worst part. The best part of this game is the story.
Now, there are plenty of games with great stories, we can all say that, but they're rarely, if ever, better than a classic movies such as One Flew Over the Cuckoo's Nest or 2001. Surprisingly, this is a game that makes those stories look like Batman Forever! Well, not to do nothing but summarize the story, there's a guy named James Sunderland, whose wife died three years ago. Then, he gets a letter from her telling him to go to Silent Hill. I feel this worked fairly well, giving James a reason that, while he CAN go back home, he feels he needs to stay and find Mary, his dead wife. This is all a good narrative on its own, but it only gets better farther along, when you're presented with new mysteries in the story. Toward the end, it becomes so metaphorical and philosophical that the creator of this game is easily the most twisted genius in history.
Okay, I think I've said plenty of good things about this game, so now I will say the bad. As I mentioned earlier, the controlls are terrible. While it's bearable on its own, the camera can confuse things, and it hides enemies that James would see easily, but the player can't.
Also, I'm a guy that plays on easy mode. I've had frustrations playing on easy mode before, since I'm pretty terrible at games, and they're still hard on easy. Didn't die once, had enough ammo to turn an elephant into a fine paste, and enough medicine to OD fifty men. Not to mention the final boss-battle, who dies in three hits. I understand easy mode being easier, but THREE. HITS. Come on, at least make it suspenseful a bit with 10 hits! I've heard normal is 15 hits, and THREE! Come on, that's... that's just weak. It actually hurt the game by removing a lot of fear, since I knew I was just plain immortal.
Objective score: 9.3: Scary, and I mean scary. Not to mention the story was thought provoking. However, it was also fairly confusing, and if you don't examine the tory closely, it wouldn't make any sense. Also, the controls and camera can get frustrating fast.
Subjective score: 10: Sheer perfection of an art form that has been struggling to gain respect from the people who don't use it (games), yet is also sheer perfection of ANOTHER art form that has respect, but now deserves even MORE respect! (horror)
Alright, I think you get it. This is my longest blog post yet, so I hope people read it!


text: When the font family is set in the Wysiwyg editor, there's the major font, then some fallback fonts for OS that wouldn't have this font. When the the message is converted to BbCode only the first font is used as key for the FONT tag, which makes easier for BbCode users to type the font directly. But when the BbCode is parsed again in html (normal html & wysiwyg parsers), the option of the FONT tag is directly used as the font family name. There could be here a callback that matches for the FONT tag option (the main font) and adds back the fallbacks fonts.


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How Can I Do This Cake And Or Cupcakes?
Decorating By Sina Updated 2 Apr 2012 , 1:08am by Evoir
Thats a great question, I would like to try this technique also.<333><
Me three! Its gorgeous!!!!
closest i can help is for you search under tutorials on this site rose petal cake and it has something similar...hope that helps
It looks to me like it starts as a traditional buttercream rose done right on the cake and cupcakes. Then just keep putting petal after petal after petal after petal.......That is a LOT of petals.
Large petal tip with buttercream, using the cupcake/cake as the base/cone for the design.
Quote by @%username% on %date%


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Z for Zachariah
by: Robert C. O'Brien
Josue Rabanales
"Mysteriously Fascinating"
• Ann Burden is all alone and feels like she is the last person in this world
•John Lemis is a scientist who finds his way to this valley and finds Ann.
•Ann leaves house and destroys her garden to not be found and also moves into a cave.
•Ann gets discovered and later finds out that the doctor is sick.
•Ann escapes and John is still there and is left alone.
Z for Zachariah
You should read it you will become connected with the characters and seem like if you are in the book you will picture all the words that are said in this amazing book. Also you will enjoy this novel since it is such a great story you will NOT regret it!!!!!


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He's expecting far different results this season.
Mercersburg has size up front and solid backcourt play. It's a combination that should provide positive results for Cubit & Co.
James Craft, a thin 6-11, 200-pound senior who has split time between Ohio and Virginia during his high school years, is attracting interest from the likes of Wright State, Tennessee-Chattanooga, Rider, Fordham and South Florida.
``He has a really strong mid-range shot, but he's not terrible gifted with his back to the basket," Cubit said. "He's getting stronger, though. He's an extremely good shooter who runs the floor well and can also alter shots on the defensive end."
He'll be joined up front by Panama native Eduardo Archibald Brown, a 6-8 power forward who will be a junior this season and has already piqued the interest of Penn State and a few WAC schools.
``He's just new to the work ethic and the amount of time we spend in the gym over here," Cubit said. "He's a big, wide kid who has a great pair of hands. He's not afraid of being physical."
Alex Tyler, a 6-7 junior power forward from Clear Spring, Md., is getting Ivy League interest.
There will be three players who rotate in the backcourt. Eric Rahauser is a 6-foot wing guard from Mercersburg, Andre Smith is a 5-11 senior point guard from Danbury, Conn., and Colin Cubit, the coach's son, is a 6-3 junior shooter. Josh Davis is a 5-10 junior guard and 6-5 junior wing Casey Oliver who will also figure into the rotation.
``This is as strong a group of inside players I've had since I've been here," Cubit said. "We have a nice compliment of guards and it's a group that lends itself to good chemistry." ``I think this is a group that will lend itself to more consistent play," added Cubit. "We had some pretty nice upsets last year, but we couldn't sustain that level over time. We're a better rebounding team this year, should have fewer turnovers and have better shot selection."


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Tina Migeot /web designer-developerI am currently working as a full time web designer-developer. I design, code, and produce small to large web sites from concept to production, working extensively with clients to create sites that suit their needs and goals.
I create clean, attractive, user-centered Web sites that comply with the latest web standards, and integrate the latest web technologies and social media platforms. (WordPress is my latest obsession - see the work I've done for San Francisco to Philly transplants, Chameleon Theatre, as an example!)
I 'm also an improviser, avid traveler, visual artist, sustainability junkie, music fanatic, and container gardener.


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Jul 21 | 18:04 IST
|Home » Videos » Union Budget
Why demonetisation had no impact on Budget 2017
BT Online | February 1, 2017
Demonetisation has not had any major impact on the Budget 2017 moreover it feels like the goverenment has continued with its theme that it has been talking about since a considerable amount of time. Even when they were getting on with thier election mode, they spoke of the same changes. These changes include: rural housing, poor, agricultural output, irrigation projects, rural road projects and more.


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All eyes are locked in on Robb Bank$ with the release of his highly anticipated (long overdue…) mixtape, Tha City. With a total of 17 original tracks, the project sports some noteworthy features in Phlo Finister ("Hotel Miami"), B.Way (of Pro Club), Dooney Montana, Lofty305 (of Metro Zu) and of course Sir Michael Rocks. The previously released records in "Trust Me" and "Practice" serve as bonus cuts, which is certainly satisfying to see happen after all. Although the production on Tha City is pretty much held down by Nuri and SpaceGhostPurrp respectfully, we do see additional assistance from Freebase, POSHstronaut, KE On The Track and even an industry heavyweight in Zaytoven. The track titles used on Tha City carry within individual city names that seem to have been of major influence (it all makes sense now, eh?). This is a bittersweet moment for the whole state of Florida, especially since this release comes on the heels of Denzel Curry's independent album, Nostalgic 64. With that said, look down below for the stream and download of Tha City (hosted by DJ Sean Bang).
October 2, 2013
CategoriesHip-HopMixtape Downloads


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» Click here to download the "Map of Light" app
Mobile access to the complete Zumtobel product catalogue is also possible via mobile devices by other manufacturers than Apple. Just go to » http://mobile.zumtobel.com on your smart phone or tablet.
Zumtobel. The Light.
» Click here to download the Lighting Handbook..
The printed version is available from your » nearest Zumtobel contact.
Zumtobel. The Light.
Zumtobel. The Light.
» Read more about SFERA and swarmControl technology
Zumtobel. The Light.
Zumtobel. The Light.
» Click here to go to the survey.
For each completed questionnaire, Zumtobel will donate € 3 to the » Light for the World charity. We would appreciate if you forward the link to the questionnaire to your friends and acquaintances. Every feedback will count!
Zumtobel. The Light.
Zumtobel has supported the integral sustainability concept by providing an integrated lighting solution: the daylight-based Luxmate Professional lighting control system ensures that natural daylight is used to optimum extent and staff members can enjoy both perfect lighting condi-tions and a pleasant atmosphere. The energy-saving concept is further enhanced by the use of state-of-the-art LED luminaires, an efficient emergency lighting system and presence detec-tors.
About the World Green Building Council (WorldGBC)
The WorldGBC is a worldwide network of national green building organisations. It aims at promoting collaboration and research as well as sharpening the profile of sustainable building methods internationally.
For more information, please refer to www.worldgbc.org
Zumtobel. The Light.
Download the latest version of ecoCALC and benefit from the new functions: » http://www.zumtobel.com/ecocalc
Zumtobel. The Light.
» Read more about the Emporis Skyscraper Award 2012
Zumtobel. The Light.
Zumtobel. The Light.
Zumtobel. The Light.
From 1 September until 30 November 2013, architecture firms and engineering studios, urban planners and landscape architects, universities and recently established technology companies, NGOs as well as public and private initiatives can submit their current projects.
Three categories
The ZUMTOBEL GROUP AWARD is awarded in the following three categories, each presented by one of the international lighting group's three brands:
• Buildings
• Urban developments & initiatives
• Applied innovations
"Buildings" category by Zumtobel
In the "Buildings" category presented by the Zumtobel brand, outstanding building projects that have been completed within the last two years (from 30 November 2011 to 30 November 2013) can be submitted. This category includes both new buildings and refurbishing projects. The award-winning project should meet the highest aesthetic demands and also involve innovative solutions for optimised use of resources, environmental protection and the improvement of living conditions. The jury will place a special focus on the application of cutting-edge technologies.
Curators and awarding of prizes
As in the previous years, the Zumtobel Group Award 2014 will be curated by Kristin Feireiss and Hans-Jürgen Commerell of Aedes Architekturforum in Berlin. From all the projects submitted, a preliminary jury will select 14 projects per category. The main jury consisting of internationally renowned experts from various disciplines will nominate five projects per category early in summer 2014 and will choose from them one award-winner in each category. The prize award ceremony will take place in autumn 2014.
For further information on the ZUMTOBEL GROUP AWARD, please refer to
» http://www.zumtobel-group-award.com
Zumtobel. The Light.
In an area of about 100,000 square metres, six internationally renowned architects have created a unique ensemble of buildings. The heart of the campus is the "Library and Learning Center" designed by Zaha Hadid, which includes a large auditorium and the main library, among other things. Grouped around it are five other university buildings designed by Hitoshi Abe (Sendai/Japan), Carme Pinós (Barcelona), Eduardo Arroyo (Madrid), Peter Cook (London) and Laura Spinadel (Vienna).
The whole campus was built in line with a so-called "green building" concept. For the various university buildings, Zumtobel developed custom lighting solutions using numerous pendant luminaires, continuous-row lighting systems, light lines and moisture-proof luminaires, and in total provided some 12,000 luminaires, in addition to a continuous row system of seven kilometres length. Zumtobel was also in charge of installation and commissioning of the luminaires, in collaboration with four electrical contractors.
For more information on the large-scale Campus Vienna project, please visit » http://www.campuswu.at
Zumtobel. The Light.
The committee is chaired by Martina Paul, CIE Secretary General. Peter Dehoff, Director International Associations and Standards at Zumtobel, is the Austrian representative on the ISO TC 274 Light & Lighting.
» For more information, please refer to the ISO website
Zumtobel. The Light.
Exemptions from the scope of application:
• Ballasts and luminaires for fluorescent and high-intensity discharge lamps (covered by EU Regulation 245/2009)
• LED modules as part of luminaires that are placed on the market in less than 200 units per year.
• For special purpose products, e.g. emergency luminaires, only product information requirements have been specified so far.
The requirements will be introduced and/or tightened in three stages (Art. 3).
• Stage 1: 1 September 2013 (general start including information regarding special purpose lamps, Annex I)
• Stage 2: 1 September 2014
• Stage 3: 1 September 2016
The requirements are set out in Annex III of the Ecodesign Regulation.
• Chapter 1: Energy efficiency criteria:
o Maximum energy efficiency index for directional lamps (1.1)
o Requirements for lamp control gear in stages 2 and 3 (1.2)
• Chapter 2: Functionality requirements
• Chapter 3: Product information requirements
Annex IV regulates the verification procedures for market surveillance purposes:
• Chapter 1 applies to lamps other than LED lamps and for LED lamps that are meant to be replaced in the luminaire by the end-user
• Chapter 2 describes the verification procedure for LED modules not intended to be removed from the luminaire by the end-user
Important ecodesign requirements according to Annex III:
Chapter 1.2 specifies the requirements on energy efficiency for lamp control gear from stage 2 as follows:
|No-load power
|Stand-by power
|starting date of application
|Lamp control gear
|n. a.
|n. a.
|Stage 2
|Lamp control gear
|n. a.
|Stage 3
|Lamp c. g. with P > 250 W
|≤ 1,0 W x P/250W
|n. a.
|n. a.
|Stage 2
|Lamp c. g. with P > 250 W
|≤ 0,5 W x P/250W
|≤ 0,5 W x P/250W
|n. a.
|Stage 3
|Halogen lamp c. g.
|n. a.
|n. a.
|Stage 2
|Functionality parameter
|Requirement as from stage 1, except where indicated otherwise
|Lamp survival factor at 6 000 h
|From 1 March 2014: ≥ 0,90
|Lumen Maintenance at 6 000 h
|From 1 March 2014: ≥ 0,80
|Number of switching cycles before failure
|≥ 15 000 if rated lamp life ≥ 30 000 h otherwise: ≥ half the rated lamp life expressed in hours
|Starting time
|< 0,5 s
|Lamp warm-up time to 95 % Φ
|< 2 s
|Premature failure rate
|≤ 5,0 % at 1 000 h
|Colour rendering (Ra)
|≥ 80 ≥ 65 if the lamp is intended for outdoor or industrial applications in accordance with point 3.1.3(l) of this Annex
|Colour consistency
|Variation of chromaticity coordinates within a six-step MacAdam ellipse or less.
|Lamp power factor (PF) for lamps with integrated control gear
|P ≤ 2 W: no requirement
Requirements for luminaires
• There are no energy efficiency requirements for luminaires
• There are no functionality requirements for luminaires
• No requirements regarding information to be provided to the market have been specified for professional luminaires, only information concerning built-in LED modules must be provided for market surveillance upon request.
All measures necessary for meeting the new requirements for light sources and for the information to be provided have been taken by Zumtobel and will be implemented in due time.
More detailed information on this topic has been made available by LightingEurope :
» LightingEurope Guide
» LightingEurope Questions & Answers
» The original text of EU Regulation 1194/2012 can be found here
Zumtobel. The Light.
Extended scope of the new regulation
While the former Directive applied to household lamps only, the scope of the new provisions has been extended to all lamps used for lighting purposes (incl. LED modules), including the following:
• lamps with "non-directional" light and "directional" light (e.g. reflector lamps)
• lamps used in professional lighting (TL, CFL-ni and HID)
• LED lamps and modules
• LV lamps and
• luminaires for the lamps listed above that are sold at a "point of sale" to "end-users" (consumers).
Please note: The EU Commission's definition of "lamp" also includes LED modules.
New energy efficiency classes and weighted energy consumption indicated on lamp labels
Energy efficiency classes (EEC) F and G were deleted, and new classes A+ and (for new LED lamps) A++ were added at the upper end of the range. The number of classes has thus remained unchanged. Equally unchanged are the limit values set for the already existing EECs A to E. In addition to the EEC, energy consumption (in kWh per 1000 h) must also be stated. The label will be placed on the lamp package as usual for end-users.
New label for luminaires marketed to end-users
What is also new is the "label for luminaires", which contains information about
• the luminaire's compatibility with lamps and information about built-in LED modules
• the range of possible energy efficiency classes (EEC)
• information about the EEC of lamps (and LED modules) supplied with the luminaire
Please note: The label for luminaires does not contain any information on the efficiency of the luminaire itself.
The label for luminaires will not be part of the luminaire package but will be presented to the end-user at the point of sale.
Effective dates:
Information about lamps marketed as individual products must be provided with effect from 1 September 2013. Information about luminaires marketed to end-users must be provided with effect from 1 March 2014.
Information regarding luminaires by Zumtobel will be added to the product data sheet on time, if not yet available. The label for luminaires will also be made available by Zumtobel to the dealers involved.
More detailed information on this issue has been made available by LightingEurope (in English):
» LightingEurope Guide
» LightingEurope questions & answers
» LightingEurope position paper
» The original text of EU Regulation 874/2012 can be found here
Zumtobel. The Light.
Click here to download the Magazine section of the sustainability report.
» In English
» In German
Click here to download the Facts section:
» In English
» In German
Zumtobel. The Light.
Zumtobel. The Light.
The Aarhus seminar was held in ARoS Aarhus Art Musuem where the guests were given a tour in Zumtobel's very own reference project Your Panorama Rainbow which was the result when the brilliant designer Olafur Eliasson and Zumtobel worked together. Here you can find the LUXMATE Professional lighting management system with the daylight sensor in the center of the rainbow.
In Copenhagen there was an even greater interest at the Bella Sky Comwell Hotel where the evening began with a welcome drink in the Sky Bar on the 23rd floor, overlooking Copenhagen. The Bella Sky is also a Zumtobel reference project and when the guests walked from the elevators to the conference rooms, they could see the LED downlight 2LIGHT.
"The interest was greater than expected and it was a joy to see so many participants in our lighting seminars. All parts of the industry were represented and we hope to be back here soon again", says a pleased Ronnie Eriksson, head of marketing at Zumtobel Nordic, who has solid experience of the industry and the Danish market.
Zumtobel. The Light.
With the SFERA free-standing LED luminaire, Zumtobel sets new standards – especially with respect to adjustability and individual lighting quality. But SFERA's energy efficiency is impressive as well, thanks to a combination of the adaptive SENSCONTROL III lighting management system and the innovative SWARMCONTROL technology used for the first time in SFERA, which allows the configuration of the luminaires into a swarm without any additional software, providing for quick adjustment to altered room layouts.
Click here for more information: » www.zumtobel.com/sfera
DISCUS Evolution
The DISCUS Evolution LED spotlight system results from further development of an existing luminaire and cannot fail to impress on account of its perfectly matched light source and optic. The spotlight's flat design is now even more contemporary and minimalist. The delicate yet robust design of the spotlight created by the internationally renowned Vienna-based EOOS design studio was created for accent lighting and the sales-boosting presentation of goods.
Click here for more information: » www.zumtobel.com/discus
This year, 1,865 manufacturers, designers and architects submitted a total of 4,662 products. The awards will be handed over and celebrated at the red dot gala at the Aalto Theater in Essen on 1 July 2013, in the presence of international guests, among them designers, business experts, politicians and media representatives. The prize-winning products will then be on display at the red dot design museum for four weeks.
Zumtobel. The Light.
In just a few steps, the SLOTLIGHT II configurator enables you to design a project using SLOTLIGHT II recessed, surface mounted and pendant luminaires, both as T16 and LED versions. In no time, the configurator shows you the best combinations for the required lengths of the continuous-row system as well as all components you need.
The software is available free of charge in German and English. The most recent version is available for downloading from our website's » Service/Software section.
» Download the software here (.zip/31,8 MB)
Zumtobel. The Light.
Comparison between MELLOW LIGHT V LED (52 W) and MELLOW LIGHT V TC-L (55 W)
The example shown in the diagram illustrates the progress in product development. Although slightly more energy is required for manufacturing the LED model, it becomes apparent that the overall environmental impact of the LED luminaire – due to its reduced power consumption – is lower than that of the comparable luminaire featuring conventional lamp technology, if the utilisation period is taken into account.
Basis of calculation
Primary energy consumption* is calculated from the energy required for raw materials and their processing, transport by truck to the customer over a distance of 1500 km and a utilisation period of 15 years in a typical office application, based on the European energy mix.
Click here to view the EPDs of the two products:
* Primary energy consumption
The term primary energy is used in the power industry to denote the energy that is available using natural forms of energy or energy sources such as coal, gas or wind. In Europe, roughly 3.3 kWh of primary energy are needed to generate 1 kWh of electricity.
Zumtobel. The Light.
At the second iF design awards night held at Munich's BMW World, four Zumtobel products received an iF product design award.
The DISCUS Evolution LED spotlight system, the ELEVO LED spotlight, the PAN recessed LED luminaire and the ONLITE PURESIGN 150 escape sign luminaire could not fail to impress the jury thanks to their outstanding product design and efficient lighting technology.
iF gold award for LED spotlight system
The DISCUS Evolution LED spotlight system won the coveted iF gold award. Entries included a total of 3011 products submitted by 1920 competitors. 981 of these products have won an award. 75 of all award winners were also given an iF gold award during the awarding ceremony.
DISCUS Evolution
The DISCUS Evolution LED spotlight system results from further development of an existing luminaire and cannot fail to impress on account of its perfectly matched light source and optic. The spotlight's flat design is now even more contemporary and minimalist. The delicate yet robust design of the spotlight created by the internationally renowned Vienna-based EOOS design studio was created for accent lighting and the sales-boosting presentation of goods.
The spotlight's innovative thermal management using a passive cooling system with radial fins is an integral part of the design concept. DISCUS Evolution is available with a black or white housing and suitable both for installation on tracks and for recessed installation.
» Click here for more information on Zumtobel's prize-winning products
Zumtobel. The Light.
Zumtobel. The Light.
1. Select the number of luminaire groups, luminaires per group and control signal (DALI or DSI).
2. Specify whether the component is to be installed in a switch cabinet or recessed into the ceiling (depends on the number of luminaire groups).
3. Is daylight-based lighting management required?4. Design of schematic circuit diagram based on possible functions.
» Click here to download the app
Zumtobel. The Light.


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Car Parts Discount stocks and sells aftermarket $makeName $modelName Universal Parts auto parts direct from our auto parts warehouse in California and other select distribution points.
OEM: 628-040, Item: 246083
Universal 12-volt seat heater kit includes one upper and one lower seat heating pad, LED illuminated HI-LOW-OFF switch, relay and fused wiring. (Kit will upgrade one car seat.) These heating pads WILL NOT FIT ALL SEATS, please verify that your seat has 12" x 12" unobstructed flat areas where you can place the heating pads. (Some seat upholstery has anchoring flaps which fit into slots in the seat foam, which can interfere with heating pad placement.)
OEM: UN6380200, Item: 125290
154 piece lightbulb kit for Audi, BMW, Ford, Mercedes, Porsche, Saab, VW, and Volvo. More Product Details: CLICK HERE for a closer look at the bulb kit chart


text: Photo Finish's High Fashion Funk Adjust content blocking imnotadj Channel: ponytime next random Comment Top NextComment On Bandcamp - yourenigma.bandcamp.com/track/da-magi... "Da Magicks" is a track I have wanted to make for quite some time. Perhaps as evidenced by Rarity being my best pony, I am definitely interested in fashion. That's not to say I am an avid follower of trends and the like, but I definitely try to dress well and enjoy me some Project Runway. So of course I'm down with Photo Finish, and so this little number was conceived. In actuality, I was going for a different feel here, so the use of the term "jazz" and even "funk" is extremely marginal. What I was going for what a sort of sound which typifies the music one hears at a fashion show, but still with the usual pony jazz characteristics (light swing, solos, etc). Samples abound in this piece; everything from Count Basie to Charles Mingus to Photo Finish's own theme. There's no explicit story being told here, but I'd like to imagine this music accompanying a montage of some sort depicting Photo Finish's rise to the top, taking the fashion world by storm with "Da Magicks!" Enjoy. next Tags: My Little Pony | Photo Finish | funk | Music +22 Views: 3596 Submitted: 09/16/2012 Facebook Twitter Google+ Tumblr Pinterest


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On 17/08/13 03:57, Eric Blake wrote:
On 08/16/2013 06:08 AM, Osier Yang wrote:RHEL6.5: https://bugzilla.redhat.com/show_bug.cgi?id=965442Links to a private bug aren't considered very nice on a public list or git repo.
Oh, I didn't notice it.
One has to refresh the pool to get the correct pool info after adding/removing/resizing a volume, this updates the pool metadata (allocation, available) after those operation are done.But at least this is a fair summary of its contents.v1: https://www.redhat.com/archives/libvir-list/2013-May/msg01957.htmlThis is helpful for review, but is generally placed...---after the separator, so that it appears only in the mail but is not incorporated by 'git am' (no one cares 6 months from now how many revisions of the patch went to the mailing list before it was polished).
I pushed with the bug and v2 uris removed. Thanks. Osier


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Hotel Kanak Palace
Call us 1800-300-2400-1 (Toll Free) and Get additional Discount upto 50% for this Hotel.
Per Room Per Night
Lowest Price Guarantee
You are getting lowest possible rate for this hotel
- About Hotel
- Reviews
Select Room
About Hotel Kanak Palace
A luxury hotel in Agra, Hotel Kanak Palace provides modern comforts and friendly atmosphere. The rooms are well-furnished and equipped with best facilities. The hotel is close to Taj Mahal, Read Fort and many other major historical places in Agra. Facilities offered here include travel assistance for sightseeing, doctor-on-call, laundry, conference hall, restaurant, room service and many more comforts.
Checkin Time: 1200 pm
Checkout Time: 1200 pm
Hotel Information and Amenities
Read More....
Hotel Amenities
- Luggage Storage
- Restaurant
- Room Service
- Travel Counter
- Parking
- Doctor On Call
- Laundry Facilities
- Front Desk
- Housekeeping
- Conference Room
- "Bottle Opener
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- Makeup Mirror
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Hotel Location


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
How do I test the Thank You page redirection?Asked by groupsing on March 08, 2016 at 06:29 AM
How do test it when i finish it?
This is a re-post of a comment on Redirecting Users to a Different Page
JotForm Support
If I understand you correctly, you would like to test the Thank You page redirection, right?
In order to test it, you need to do an actual submission on your form to see if it will redirect properly. Once you fill up the form and hit the submit button, it should redirect to that page URL.
Let us know if you have any questions. Thank you.


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John Havens: Citi's New President
Citigroup is overhauling its structure.
Vikram Pandit named John Havens as president and chief operating officer. The move is part of a structural overhaul meant to address the bank's efforts to expand as it tries to put the lingering effects of the financial crisis behind it.
The promotion of Havens, a longtime colleague of Pandit's, will cut the number of executives reporting directly to Pandit by half from a current unwieldy 18 and should relieve him of some front-office responsibilities.
The 54 year-old Havens has led Citi's institutional clients group for the past three years.
Below is the message Pandit sent to Citi employees. Click here to read the news release and here to see Citi's new organizational chart.


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In one of the most iconic superhero films of all time, Batman Begins, Bruce Wayne's father says, Why will we fall? Hi rosieannemayers, thanks for dropping by. It's good that you're monitoring your health by checking your urine coloration on a regular basis. Based on costs paid by public hospitals and personal operators abroad, the private medical health insurance industry argues it is forced to pay anyplace between $seven hundred million and $1 billion greater than it ought to to cover the price of devices like coronary heart valves and replacement hips utilized in surgeries. Although that is often a minor procedure, it's usually medically wanted if a mole appears to be precancerous or can in any other case cause well being issues. Where -i there are any – are these firms hiding – there are actually none in Ireland. According to World Health Organization, exposure to WiFi and cell phone radiation under international guideline limits does not end in any antagonistic well being effects.
While this isn't the kind of surgery one thinks of in terms of cosmetics, it certainly addresses the emotional look and physical look of those who need it. It is also comforting to know that insurance coverage companies usually acknowledge it is a medically needed procedure. The Office of Disease Prevention and Health Promotion (ODPHP) performs an important function in preserving the Nation healthy.
That included an accusation that it acquired kickbacks from Johnson & Johnson for recommending that doctors prescribe to nursing residence sufferers the antipsychotic Risperdal, which might hasten dying in aged individuals with dementia. This option performs a key role in distinguishing SHOP from the surface medical insurance market, and it's popular among small business owners. The Advisor: Journal of the National Association of Advisors for the Health Professions, 366(three), 9-thirteen. Queensland Health has committed to the establishment of a digital health system and Cairns Hospital is a crucial a part of that system," she said. Canada ranks twenty fourth out of 25 developed international locations for environmental health and sustainability. BUT the companies I work for (yes, I work 2 full time jobs) would not have what they name light duty, one handed" jobs for my place.
The undeniable fact that the surgery makes you look higher, or feel higher about your seems to be, is secondary to the well being advantages it may well provide. Which means if You choose this business, You can even be capable to gain again your Health and earn your Financial and Time Freedom. Probiotics is the one complement that can construct a healthy digestive system and a healthy digestive system is essential for good well being.
HCF, Australia's largest not-for-profit well being fund, has launched information from a survey of 35,000 of its members, carried out over the past three years, that highlights a major disconnect between member expectations and precise experiences in public hospitals. Distorted policy, legislative andclinical priorities; a loss of money from well being techniques; denied or delayed access to health care providers and therapy; and a loss of trust in national health amenities, personnel and to a wider extent nationwide governance as a whole. Before the cycle of alcohol addition happens, folks have to change into educated about alcohol use and its well being concerns. Companies which are accepted to Blueprint Health are identified within the trade for the quality of their companies and their administration teams.


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I'm taking part in this group exhibition in the Bolin Bolin Gallery.
It runs to Christmas Eve – but will then continue until the end of January. Continue reading »
I'm taking part in this group exhibition in the Bolin Bolin Gallery.
It runs to Christmas Eve – but will then continue until the end of January. Continue reading »
Ceramic Sculpture with Meredith Plain.
One Day Workshops for Adults
Ceramic Sculpture Workshop – Dragons and other creatures
Sunday 5th March 2017
To book, or find out current dates Click Or, go to http://gallery.baag.com.au/?page_id=5 Continue reading »
I will be taking part in this exhibition in August
An exhibition inspired by trips to Japan led by Linda De Toma and Naoko Coghlan focussing on ceramics and textiles
5 August-4 September Continue reading »
In 2014 I was lucky enough to take part in a tour of Japan led by Linda De Toma, (founder of Claydreamers studio, who is a ceramic artist with a passion for Japan and Japanese ceramics) and Naoko Coghlan (a potter who grew up in Japan but now has her home in Melbourne). Continue reading »
After missing last year I am once again taking part in the ASV (Assoc Sculptors Vic) exhibition at MIFGS (Melbourne International Flower and Garden Show) in the beautiful Carlton Gardens. This exhibition introduces sculpture to the thousands of people who love their gardens.
Saturday the 5th December 12-3pm
Saturday the 12th December 11am-2pm
By ceramic sculptor and Bolin Bolin Gallery manager Meredith Plain.
Tour the Christmas exhibition and find pout about the artists featured. Continue reading »
I am once again taking part in the interesting and varied exhibition in the
Bolin Bolin Gallery at Bulleen Art & Garden.
6 Manningham Rd W., Bulleen. 88503030.
Continue reading »
I will be giving an Artist Talk and Demonstration
Friday, November 6, 2015 (10:30 am – 12:00 pm) Continue reading »
A giant rooster and a hen have nested at Bulleen Art & Garden and now oversee the driveway . They were installed in December 2014.
They are representative of food gardening, which is very important at Bulleen Art & Garden and also a big part of my life. Continue reading »


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Katja Kassin: Aka Filthy WhoreFavorites [+]
Pay-Per-Minute Viewing
You can now enjoy this movie on your iPhone®, iPad®, or compatible Smart TV & Android devices.
Purchase Options
Pay-Per-MinutePurchase Minutes
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Available MP4 sizes:SD 480p (1.4 GB)
DRM-free downloads offer content that is not limited by standard Digital Rights Management (DRM) access technologies. These technologies allow hardware manufacturers, publishers, copyright holders and individuals to limit the usage of digital content.
When you purchase DRM-free downloadable content, that content is available for you to download on any computer and save to a disk whenever you like. You can also download to multiple computers. There is no timeframe associated with your purchase and the content will not expire.
Running Time: 92 minutes
Released: 01/2006
Studio Name: Legend
Series: ... aka Filthy Whore
Director: Kidd Tea
Categories: All Sex
Description: Katja Kassin is a stupidly foreign redhead with only one aspiration in life to achieve the title of filthy whore. Watch Katja as she fulfills her quest for cock and cum.
5 Scenes from Katja Kassin: Aka Filthy Whore
Watch with a Subscription to the SuperPass Channel, with Pay-Per-Minute or Streaming Rental
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|Cloud: I couldn't finish 'em. Looks like this's gonna get complicated.
|The following tables are incomplete and require the Easy Type information areas to be filled. If you wish, please examine the table and add anything missing. Remove this notice upon completion.
The Summoner, also known as the Conjurer, is an enemy in Final Fantasy IV that appears in the Passage of the Eidolons.
Like other enemies of its type, the Summoner can call forth its own minions if left alone, so it should be defeated quickly, unless one wants to grind for EXP and gil. It can summon Goblins, Hellflappers, Nagarajas, Arachnes, or Thunder Dragons to its side during battle.
AI scriptEdit
This article or section is a stub about an enemy in Final Fantasy IV. You can help the Final Fantasy Wiki by expanding it.
Other appearancesEdit
Pictlogica Final FantasyEdit
This article or section is a stub about an enemy in Pictlogica Final Fantasy. You can help the Final Fantasy Wiki by expanding it.
Related enemiesEdit
Final Fantasy IV -Interlude-Edit
Final Fantasy IV: The After YearsEdit
Evocation is the act of calling upon or summoning a spirit, demon, god or other supernatural agent, in the Western mystery tradition. Comparable practices exist in many religions and magical traditions and may employ the use of mind-altering substances with and without uttered word formulas.


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This is my first blog post here, so I ask your forgiveness if I violate any blog protocols that I am likely ignorant of.
I wanted to post an idea I had regarding The Numbers to see what people think. My theory is that the numbers will change but their sum will stay the same before the end of the show. This would be consistent with the idea of progress that Jacob stated; perhaps this has been going on longer than we know (nemesis: "they kill, they destroy, they corrupt. It always ends the same.")
It may be the point of the writers/creators of this show to demonstrate that we are free to be the people that we are (free will is valid), AND that certain outcomes are simply a given in the grand scheme (fate is also valid). I'll further stipulate that the Temple is the last Dharma experiment in an attempt to manipulate these numbers, and that the Losties will be using it in some way to interact with themselves in an alternate timeline (created by the detonation of Jughead) to either restore or alter events. In the end, the numbers will change due to a failure in accomplishing this goal (although they will likely get close).
To this end, I even feel I have a possible candidate for what the new numbers might be: 2 3 16 18 24 45 These numbers share much in common with the existing set of numbers already established in the show. Among such elements include: A. Same composition of digits B. Two odd numbers C. Sum to 108 D. Consecutive flight numbers pertinent to the show (i.e. 8 15 vs. 3 16). E. Two single digits, two teens, one twenties, and one forties.
I encourage anyone to share their thoughts on this or any other related aspect of the show!


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Mcleod Shelby GT500 RXT Street...
Mcleod 2010 GT500 RXT Street Twin Disc Clutch Kit (Street) Includes: Mcleod RXT Clutch Mcleod Lightened Steel Flywheel NOTE: The 2010 GT50...//Shop Now
You asked for it and VMP Tuning and Superchargers delivers! The pinnacle of TVS technology is now the foundation of power for your '11+ 5.0L Mustang! And this is as hot as it gets! Stage 3, ready to rock and is E85 ready, right out of the box!
VMP GEN II 2.3L TVS for '11-'15 GT 5.0L & Boss 302. STAGE 3 including ID1000 fuel injectors, VMP Plug N Play fuel pump booster, VMP Twin Jet 67mm throttle body, Dual Fan heat exchanger and VMP CUSTOM TUNES!Kit Includes:
By ordering this tune, you agree to assume all responsibility for assuring that it is to be used for off-road and/or competition use only and is not to be used on a vehicle that is registered for use on public roads nor highways.
Because U.S., Canadian, and other international, state or provincial laws and regulations may prohibit removal or modification of components that were installed on vehicles by Ford Motor Company to meet emission requirements or to comply with motor vehicle safety regulations applicable to vehicles manufactured for use on public roads, VMP wants you to know the following:
Most of the parts and services offered in this online catalog are for off-road use or competition use only. Use on State and Federal Highways may be a violation of the EPA Clean Air Act. The Clean Air Act can be found at http://www.epa.gov/air/caa/. This document contains in detail what are considered to be violations of the CAA and corresponding penalties for failure to obey and should be read in full before purchasing or installing this off-road or race use only product. Ensuring that all emissions, noise/sound, and speed/use related laws are followed is the responsibility of the Buyer(s). Installation and use of this product indicates that this disclaimer has been read, acknowledged, and understood fully by both the Buyer(s) and Installer(s). The Buyer(s) assume all associated risk of the purchase.Emissions Notice:
Federal and several state and provincial laws prohibit the removal, modification or rendering inoperative of any part that affects emissions or safety on motor vehicles used on public streets or highways. VMP assumes no liability for any violations arising out of any federal, state or provincial emissions or safety requirements on motor vehicles arising out of the customers modifications and/or use of our products or services. It is the responsibility of the customer to determine if their modified vehicle complies with applicable laws.California Residents
The emissions laws and regulations of the state of California apply to all non-racing vehicles. Consequently, some parts in this catalog may legally be used in California only on a racing vehicle which will never be operated on public roads.
|VMP Tuning


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Catherine Cassidy, Taste of Home Editor-in-Chief.
Launched in 1993, Taste of Home is the most popular recipe magazine in the world, with nearly 4 million paid subscribers. The delectable, practical recipes—more than 60 in each issue—are family favorites contributed by Taste of Home readers. The dishes, all beautifully photographed, capture special flavors of the season and are made with ingredients that home cooks are likely to have on hand or can readily find at a local supermarket.
New recipe contests are announced every issue, and readers are encouraged to send their best recipes for Taste of Home's regular features, like "A Complete Meal in a Matter of Minutes," "Cooking for One or Two," "My Mom's Best Meal," "Super Snacks & Appetizers," and "Just Desserts."
The magazine's unique staff of field editors is made up of more than 1,000 experienced home cooks located in each state and Canadian province, who regularly share recipes, well-liked menus and kitchen tips. Taste of Home is published six times a year and has five sister magazines, Simple & Delicious, Slow Cooker, Appetizers, Cooking for 2, and Healthy Cooking.


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Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
As each tradesman expresses interest in quoting on your job, we'll send you their profile and ratings to help you choose the right one for you.
After the work is complete, return to the site and rate your tradesman based on their quality of work, reliability and value for money.
Carpenter / Joiner
Added 15/05/2017
Two wooden handrails up to my lodge in Mullacott. One was rotten, and I have removed all but...
Gardener / Garden Designer
Added 13/03/2017
Services required: Remove bags of soil and bags of rubble (not mixed up) Approximate length...
Painter / Decorator
Added 03/01/2017
Services required: painting 3 x bedrooms, 2 x dining rooms, 1 x lounge/living room, 2 x bathrooms,...
Added 21/10/2016
Service gas appliance 1 x boiler, 1 x gas fire Additional information: I'm looking to get...
Heating Engineer
Added 28/09/2016
General service of gas boiler
Carpenter / Joiner
Added 02/03/2015
I am looking to have a covered decking build attached to the house at the back garden. Size:...
Managing your job is easier with the Rated People Homes App, download it today.


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- Author: Michele Campeotto <micampe at gmail dot com>
- Download: swiftbrook.tar.gz
Description Edit
Oh No! Yet another clone of a BlackBox theme! Well, actually I have cloned a modified (by me) version of the original theme by ender <zakj at i dot am>.
Looks good with a plain background color: R:0.36 G:0.45 B:0.49
Screenshots Edit


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… management apps, such at Full Circle. Go beyond traditional single touch attribution and measure campaign influence While you can track last touch attribution natively in Salesforce, marketers often need a deeper understanding of their campaign's performance. It is rare that one campaign will be responsible for the creation of an opportunity. Apps…