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Gore Worries House Democrats
By Ceci Connolly and Juliet Eilperin
Participants in the closed-door session said some members complained that the Gore team is neglecting key Democratic constituencies such as unions, minorities, inner-city residents and blue-collar workers. A few also challenged the administration's Kosovo policy, worrying that a protracted overseas conflict will detract from the party's domestic agenda for the 2000 campaign.
When Rep. Robert T. Matsui (Calif.) pressed Gore's aides for assurances the White House will not leave them out of critical Social Security negotiations, many in the room applauded, said two people in the meeting.
"The vice president should make clear very soon what his candidacy is all about," said Rep. Neil Abercrombie (Hawaii), who spoke on behalf of public sector employees and urged an end to the war in Yugoslavia. "The president should be gearing down, and the vice president should be gearing up."
One Democratic aide attributed the questions to "lingering suspicions" on Capitol Hill about President Clinton. "The question is whether Gore will pay a price for that."
Rep. Jesse L. Jackson Jr. (Ill.) said lawmakers need to speak to the people who have "Gore's ear" on substantive policy matters, and not the campaign "mechanics." He reiterated concerns he had written to Gore's campaign manager about two weeks ago. In the letter, he said Gore can separate himself from Clinton by targeting his economic message on those who have not shared in the good times. "In my district, the economy has not improved during the Clinton administration," Jackson wrote to Craig Smith. Weak spots in the economy, Jackson predicted, "will fuel apathy and depress voter turnout."
The meeting highlighted splits in the party. While Jackson complained about reports that Gore -- with the help of the Democratic Leadership Council -- is crafting a centrist campaign message, others embraced that approach.
"He's forming his message in such a way that it resonates in the Democratic strongholds and independent suburban areas," said Rep. James P. Moran (Va.), who described the session as "a mutual love fest" with just a "little contention."
About 50 Democrats attended the briefing with Smith, media adviser Bill Knapp and pollster Mark Penn, who stressed Gore's large lead in primary poll matchups with Democrat Bill Bradley. "We've eliminated our strongest opponents, our fund-raising is doing well and now we are entering a new more aggressive phase of the campaign," Knapp said, according to one account.
House Minority Leader Richard A. Gephardt (Mo.), who considered challenging Gore, urged colleagues to campaign for Gore back home as well as in Iowa and New Hampshire. Acknowledging Gore's campaign "could be better," Gephardt said he is pleased to see more attention on "getting better organized in the early states."
Rep. Maxine Waters (Calif.), echoing Penn's presentation, said the popularity ratings of Texas Gov. George W. Bush (R) are bound to fall. "When voters understand who he is and his relationship to the right, they will not be so enamored by him," she said.
Several lawmakers said they are eager to run with Gore on popular issues, such as education and health care. Waters and others estimate 170 of the 211 House Democrats support Gore.
© Copyright 1999 The Washington Post Company

subset: commoncrawl


text:
Delhi Protests – Lumpens In The Media
A few top guys in an office on the sixth floor of a semi-govt company used to work late often. This was at Nariman Point in Mumbai. So these 4-5 guys use to dip their hands in the office petty cash and order beer. The peon used to go and get the stuff for them. It was a regular practice and was on for years. The vouchers were made with fake bills and the auditors never found anything wrong or unusual in the expenses. This went on for 7 years till a junior auditor came along and found some vouchers with unfair expenses. The peon regularly claimed taxi fare to and from the company's bank for daily transactions. On seeking a clarification from the manager, the auditor got the reply that there are no autos around Nariman Point and buses take a long time so it was fair for the peon to take a taxi every day. The junior auditor said "no, no, I'm asking why is he claiming taxi fare and why does he need a taxi". The irritated manager again explained the difficult situation around Nariman Point with commuting. The auditor then told him: "But sir, your office is on the 6th floor and your bank is on the ground floor, so why a taxi at all"? Oops!If the management guys are looting the company then they have to take care of the little guy. They have to allow the peon his little share of looting. That's a reasonably good value system, isn't it? Be fair in looting! It's a value system that govts share with the media too. The govt is the big guy and some channels are like the peons. So if the govt is into misdeeds then it allows some news channels to have their share of misdeeds and corruption. These news channels are the peons of the management in the govt. It is rare for the little guy to expose the misdeeds of the boss. That driver screwing his boss with a video of his sexcapades is an oddity. The guy was an unusual lousy boss who didn't pay well for his secrets. But not the govt peons from the media, they're paid well. They are paid well not only to keep secrets but also to defend the govt no matter what. The story in the first para is neither a joke nor fake. It's time to take a detour.
On December 22 people of Delhi were out on the streets protesting the brutal gang-rape and assault of a 23-year old woman on a bus. Thousands were out at India Gate and up the path to Rashtrapati Bhawan in protest. Their outrage was justified and the protests seemed spontaneous. Delhi-ites are sick to the bone with crimes, especially rape and assaults on women. Yet someone in the media could only hear of "drunken lumpens" among the protesters. You know, like lumpens who rape and assault women on buses and throw them on to the streets to die. The police had to lathi charge some of these lumpens. Yeah, why not! Take a look at the two pics below:
One is of a gentleman using a lathi and destroying the AmarJawan memorial in Mumbai during the Azad Maidan protests in August 2012. The police were kind to the civilised protesters at Azad Maidan then. The second pic is that of a lumpen element being lathi-charged by a policeman. You can take one look and tell that the lady being beaten up is drunk. Can't you? Well, many such "drunken lumpens" had infiltrated the protesting crowds in Delhi so naturally Barkha Dutt was very disturbed. She went on a tweet-spree about these lumpens.
Barkha got feedback from her reporter that drunken lumpens had infiltrated the crowds and was naturally keen to let her friends know about the dangers. She first tweets quite pragmatically. Then she tweets to another friend called Prasanto. Both Barkha and Prasanto are "rabid mutual ass-lickers". Don't ask but I can tell these things. So it is safe to assume that the guy at Azad Maidan is a nice gentleman because he is "secular" and the lady being beaten up by the policeman is probably a drunken lumpen. In any metro city it is quite likely there are always certain lumpen elements hanging around in some corner, lurking for the moment and their prey. They can be on the streets, on trains and buses and even in media studios. So why is it so shocking for Barkha? No, wait! First, outside Safdurjang hospital it's only drunken lumpens and no protesters. Dangerous, isn't it? But on second thoughts, in the next tweet, she makes concessions: "some of the crowds" are lumpens, not all.
Chaprasis have to often defend their bosses at all times. They may have various incentives to do so. If necessary, they can create suspicion, spread alarming falsehoods and even outright lies. It comes with the job. You try and point a finger at their boss and you will find yourself painted as the most uncouth, uncivilised character that possibly could be. Sometimes it can be mere loyalty. Let's move on.
So the friend tweets and asks Barkha "how will social and physical disorder in public spaces achieve public order"? Good question! And Barkha instantly agrees.
"Exactly" she says, like she was making the same point. Considering drunken lumpens had infiltrated the crowds the danger of some impending disaster was palpable. The protest was for security of women, to prevent crimes against women and it would be a tragedy if some crime were to happen during the protest itself. Wouldn't it? I guess, they should have first consulted Barkha and her cronies on what would be the best way to protest. After all, Barkha does have experience of being in the midst of protests and reporting, doesn't she? No, no, not from Jantar Mantar or India Gate but from another place. It's called Tahrir Square and it's in ancient Egypt. These people should have learned from Barkha about "secular" protests and how to treat women and stuff.
In February 2011 Barkha tweeted and dismissed fears about the Muslim Brotherhood, the radical Islamic group, taking over Egypt. Barkha and her Fiberals were convinced Egypt was turning into a liberal democracy. But that's hardly the point, eh? The point is about those loverly protesters at Tahrir Square. "Woh Tahrir waale kahan aur yeh India Gate waale kahan". No comparison at all. Tahrir wins hands down. You see, at Tahrir the young people cut across all class types. Oh, not to forget, they were "secular". Which in the words of our Fiberals means they are cultured and well-behaved, unlike the uncivilised lumpens at India Gate or Safdurjang who deserved to be lathi-charged and water-cannoned by the police. Egypt, as an Islamic majority nation, frequently targets Coptic Christians for violence, burns their churches and is now turning into more of an Islamic state than it ever was before. But to Barkha, they are "secular" and there were apparently no lumpen elements in all the crowds she met. Is that really true? And despite the massive presence of Army at Tahrir who didn't assault the crowds, our police with their water-cannons and lathis were far more civilised. Right?
We had our drunken lumpens in the bus who raped and have nearly murdered a woman.Lara Logan of CBS, a woman who belongs to Barkha's professional community, had a very different experience though. On February 11, around the same period Barkha was in Egypt, Logan was attacked by Barkha's "secular" crowds at Tahrir. She was assaulted and molested. In Logan's own words: "For an extended period of time, they raped me with their hands. My clothes were torn to pieces…what really struck me was how merciless they were. They really enjoyed my pain and suffering. It incited them to more violence". Writing anymore on this incident would be too graphic. But hey, they weren't drunken lumpens like the ones at Safdurjang or India Gate and they were "secular". Of all the Tahrir Square reports, I'm not sure I've read one from Barkha about Lara Logan.
Someone mentioned the term 'MacaulayPutras' to describe the elites. The Delhi protests brought out the contempt that one suchMacaulayPutri, Barkha Dutt, holds for ordinary people when they are up against the govt she serves through her channel. Oh yes, if the govt is the boss it is fair to call NDTV the Chaprasi. The Chaprasi that will speak for, twist and spin issues on behalf of the govt. Not surprisingly, the only ones seeing lumpens are from NDTV asMaya Mirchandani tweeted. And the guy the channel was speaking to when the lathi-charge and water-cannons fired? Of course, MoS for Home, the Honourable RPN Singh! Yes, the same guy who kicked people on November 14, 2011 when RahulG started his UP campaign at Phulpur. In any other democracy RPN would have been in jail but he's a minister in the Congress govt and, of course, Barkha is a Padma Shri.
Even a foreign journalist,Andrew Buncombe didn't miss it. Vishnu Som, who was on the ground and reporting, perhaps suggested the 'protesters have lost the plot'. Like most ordinary people even Buncombe didn't miss the disgusting moronery of NDTV. He tweeted to call NDTV govt spokies. He is being kind. I have called them for what they are; peons. On December 22, the real drunken lumpens weren't on the streets; they were in the studios of NDTV and in the heads of their propagandists, led by their chief-propagandist.
Source: Media Crooks
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Implantation of undifferentiated and pre-differentiated human neural stem cells in the R6/2 transgenic mouse model of Huntington's disease
Neurobiology of disease
Cell therapy is a potential therapeutic approach for several neurodegenetative disease, including Huntington Disease (HD). To evaluate the putative efficacy of cell therapy in HD, most studies have used excitotoxic animal models with only a few studies having been conducted in genetic animal models. Genetically modified animals should provide a more accurate representation of human HD, as they emulate the genetic basis of its etiology.
In this study, we aimed to assess the therapeutic potential of a human striatal neural stem cell line (STROC05) implanted in the R6/2 transgenic mouse model of HD. As DARPP-32 GABAergic output neurons are predominately lost in HD, STROC05 cells were also pre-differentiated using purmorphamine, a hedgehog agonist, to yield a greater number of DARPP-32 cells. A bilateral injection of 4.5x105 cells of either undifferentiated or pre-differentiated DARPP-32 cells, however, did not affect outcome compared to a vehicle control injection. Both survival and neuronal differentiation remained poor with a mean of only 161 and 81 cells surviving in the undifferentiated and differentiated conditions respectively. Only a few cells expressed the neuronal marker Fox3.
Although the rapid brain atrophy and short life-span of the R6/2 model constitute adverse conditions to detect potentially delayed treatment effects, significant technical hurdles, such as poor cell survival and differentiation, were also sub-optimal. Further consideration of these aspects is therefore needed in more enduring transgenic HD models to provide a definite assessment of this cell line's therapeutic relevance. However, a combination of treatments is likely needed to affect outcome in transgenic models of HD.
Despite very significant advances in understanding the causes of Huntington's disease (HD), an efficacious therapy remains elusive
During the last decade, neural stem cell lines emerged as a potential alternative to fetal tissue grafts, as they can be maintained and expanded in vitro. Human neural stem cells (hNSCs) afford a sustainable and scalable homogenous cell source to treat large cohorts of patients
However, to successfully progress this approach to a routine clinical application, it is essential to develop this approach for human stem cells
Human neural stem cell line (STROC05)
The cmyc-ERTAM conditionally immortalized human striatal neural stem cell line (STROC05, kindly provided by ReNeuron Ltd., Surrey, UK) was previously described
In vitro differentiation of STROC05 cells
To induce neuronal differentiation and increase the proportion of DARPP-32 cells, STROC05 cells were grown
Effect of harvesting on cell viability and differentiation
As differentiated cells are very vulnerable when removed from tissue culture flasks, it is essential to establish whether harvesting these cells after long-term differentiation affects their viability and differentiation status. For this, cells were harvested with Trypzean EDTA for less than five minutes at 37°C, followed by adding a soybean trypsin inhibitor to inactivate the enzymatic activity. After harvesting, cells were centrifuged for 5 minutes at 1500 rpm and the cell pellet was re-suspended in 1 ml of DMEM. Using the trypan blue exclusion test, cells were counted and viability was established to be 89.5%. Cells were re-seeded on laminin-coated cover slips in 24 well plates at 100,000 cells per well. After 24 h, viability of these re-seeded conditions was evaluated again using the live/dead stain (viability/cytotoxicity kit for mammalian cells, Gibco) and compared to cells that were not harvested. For the live/dead stain, media was aspirated and cells were washed once with PBS prior to incubation with 2 μM calceinAM (to detect live cells) and 4 μM ethidium homodimer-1 (EthD-1) (to detect dead cells) in PBS (500 μL per well) for 45 minutes at 37°C. Photos were taken immediately using a fluorescent microscope (Zeiss). A separate set of coverslips were fixed with 4% Parafix (Pioneer) for 5 min. Immunohistochemistry was used to establish if harvesting of cells would affect the proportion of neurons (1:500, mouse anti-β-III-tubulin, Tuj, AB7751, Abcam) and specifically DARPP-32 neurons (1:500, rabbit anti-DARPP-32, AB1656, Chemicon) within the cell suspension. After overnight incubation (at room temperature) with the primary antibody, an appropriate secondary ALEXA594 (1:1000, Molecular Probes) was applied for 60 min prior to attaching the coverslips to microscopic slides with Vectashield for fluorescence containing DAPI (Vector Laboratories). Total DAPI, as well as Tuj and DARPP-32 cells, were counted under a Zeiss Axioscope.
R6/2 mice
All procedures of this study were carried out according to the UK Animals (Scientific procedures) Act 1986 (PPL70/6445), as well as the ethical review process of King's College London. A widely used and well characterized mouse transgenic model of Huntington's disease, R6/2 mice present with a rapid disease onset that is evident as early as 6 weeks of age. Especially the development of a clear behavioural phenotype in the R6/2 compared to the N171-82Q or HDH111 is important to establish a potential therapeutic efficacy.
The average life span of R6/2 mice with 210 CAG repeats is approximately 16 weeks of age
Forty female mice were randomized into 4 groups; wild type mice receiving vehicle (WT-veh, n = 10), R6/2 mice receiving vehicle (R6/2-veh, n = 10), R6/2 mice receiving undifferentiated cells (R6/2-undiff, n = 10), and R6/2 mice receiving long-term purmorphamine-differentiated cells STROC05 (R6/2-diff, n = 10). Mice were group-housed 4 per cage containing mixed genotypes (one from each experimental group) to ensure comparable standard housing conditions, as described by Hockley et al.
Cell implantation
On the day of transplantation, the cells were harvested by incubation with Trypzean EDTA for less than five minutes at 37°C, followed by adding soybean trypsin inhibitor to inactivate the enzymatic activity. After harvesting, cells were centrifuged for 5 minutes at 1500 rpm and the cell pellet was re-suspended in 2 ml of DMEM for cell counting. Cells were suspended in vehicle consisting of 2.5 ml of DMEM and 3.75 ml of Hypothermosol (BioLife Solutions) at a concentration of 7.5 × 104 cells/μl. Using the trypan blue exclusion test, viability was determined to be 89%.
At 7 weeks of age, mice underwent stereotactic surgery for the injection of NSCs. This allowed sufficient time to conduct pre-implantation MRI scans, as well as behavioural test, after animals were weaned at 4 weeks of age from their mothers. Additionally, animals' genotype was determined and animals were randomly allocated to their experimental groups based on a sequence of random numbers. Although at 7 weeks of age, R6/2 mice do not exhibit a motor deficit
For cell implantation, anaesthesia was induced through isoflurane inhalation (Abbott) at 4-5%, then maintained at 1.5-2%. Animals were mounted in a stereotaxic frame and a sagittal incision was carefully made followed by the drilling of two burr holes. Either 6 μl (3 μl per side, 0.5 μl/min) of cells or vehicle were injected with a 22 Gauge needle attached to 10 μl Hamilton syringe using a convection-enhanced delivery
Additional file 1
Figure S1. STROC05 survival in the 3-nitroproprionic acid (3-NPA) rat model of Huntington's disease. Male Lewis rats (220-250 g) received i.p. injections of 42 mg/kg 3-NPA. (Sigma-Aldrich) for five consecutive days to induce a bilateral degeneration of striatal cells, as previously described
Click here for file
Additional file 2
Figure S2. Acute survival of STROC05 in WT mice. An injection of 225,000 STROC05 cells in 3 μl (75,000 cells/μl) at 7 weeks of age into wild-type mice resulted in a good graft survival (Human nuclei antigen, HNA, in red, DAPI in blue), even in the absence of immunosuppression. Cells remained within the injection tract and did not exhibit any migration out of their site of injection. To ensure a better distribution of cells within the striatum, two deposits were placed within the same injection tract. A glial reaction (GFAP + cells in green) was evident along the injection tract. These results indicate that STROC05 cells can survive in WT animals and that using this protocol there is a robust engraftment.
Click here for file
After surgery, post-operative care included fluid-replacement (0.1 ml saline/animal) and a local analgesic (EMLA cream 5%; AstraZeneca, UK). The animals were singly caged with softened food pellets and water available
Body weight
Weight loss is a prominent symptom in R6/2 mice
Behavioural battery
For each behavioural test, the running order of animals was based on a randomization of the cages, but within each cage (containing WT and R6/2), mice were run sequentially. Animals within each cage were randomly chosen for each trial. If more than one trial was conducted, this was run in the same sequence.
The rotarod is considered a very sensitive and reliable motor task to assess motor coordination in HD transgenic mice
Open field
The open field test has been used extensively as a reliable measure to evaluate locomotor activity and anxiety-like behaviour in R6/2 mice
Grip strength
Grip strength analysis is a reliable and sensitive test to evaluate muscular strength in R6/2 mice
Magnetic Resonance Imaging (MRI)
Six weeks following cell implantation, mice were anesthetised using isoflurane (4-5% induction, 1.5-2% maintenance in 0.7 l/min medical air and 0.3 l/min oxygen) and fixed within a head holder/respiration mask to reduce head movement. MR images were acquired using a 7 Tesla magnet (Varian), equipped with a 100 Gauss gradient set and a 39 mm transmission/receive coil (Rapid). A T2-weighted multi-echo multi-slice (MEMS) sequence was used (TR = 2500 ms, minimum TE = 10 ms, number of echo = 8, echo spacing = 10 ms, averages = 4, matrix = 128x128, and FOV = 20 × 20 mm). Thirty coronal slices with 0.5 mm thickness were acquired across the mouse brain. Manual segmentation of anatomical regions of interest (ROIs, Additional file
Additional file 3
Figure S3. Representative T2x-weighted MRI images. Images illustrated the anatomical boundaries used to define regions of interests corresponding to anatomical structures (red lines).
Click here for file
After MRI scanning, anesthetized animals received an intracardial perfusion of saline followed by 4% Parafix (Pioneer). Brains were excised and post-fixed for 24 h at 6°C before being cryoprotected in 30% sucrose at 6°C. Sections (40 μm) were cut on a freezing sliding microtome in the coronal plane and stored at −20°C in tissue cryoprotective solution (25% glycerine, 30% ethylene glycol, and 50% PBS).
To identify transplanted cells, sections were stained with a mouse anti-human nuclear protein (HNA) antibody (1:400, MAB1218, Millipore). For this, sections were rinsed with PBS, blocked for 30 minutes in 0.1% H202 as inhibitor for endogenous peroxidase activity (Sigma), followed by 60 min incubation in 10% blocking solution (10% normal goat serum in 0.3% Triton X-100 PBS) at room temperature (RT, 21°C). To block the non-specific binding of endogenous biotin, the sections were incubated with avidin-biotin blocking solutions (Vector) for 30 min. The sections were incubated with the HNA antibody at RT for an hour, followed by 10 min of incubation at RT with secondary biotinylated anti-mouse antibody (1:200, Vector), and 5 min at RT with an avidin-biotinylated-peroxidase complex (1:100 in PBS, Vector). Secondary antibody binding was visualized using 3,3'-diaminobenzoic acid (DAB, Sigma) dissolved in PBS with the addition of H202 to a concentration of 0.03% immediately before use. Finally, the sections were washed in PBS, mounted onto glass slides, dehydrated for 5 min in each of 70, 85, 90, and 100% alcohol, cleared by xylene, and coverslipped with Entellen (Merck, UK).
For fluorescence immunohistochemistry, sections were incubated for 60 min in 10% blocking solution (10% normal goat serum in 0.3% Triton X-100 in PBS) at RT, followed by 30 min of an avidin-biotin blocking solution (Vector). Sections were then incubated with appropriate primary antibodies against transplanted cells (mouse anti-HNA 1:400), neurons (rabbit anti-Fox3, 1:500, ab104225, Abcam), or DARPP-32 neurons (rabbit anti-DARPP-32, 1:500). After overnight incubation with the primaries, an appropriate secondary antibody (1:200, ALEXA 350; 1:500, ALEXA 647, Molecular Probes) was applied for 60 minutes at RT. Sections were rinsed in PBS and mounted in Vectashield with DAPI.
Statistical analyses
Statistical analyses were conducted using GraphPad Prism 5.03 (GraphPad Software, San Diego, California, USA) to determine significant differences (p < .05) between
Differentiated cells retain viability and DARPP-32 phenotype after harvesting and re-seeding
To assess whether transplantation of long-term differentiated cells is possible, long-term differentiated cultures were harvested and reseeded to measure potential effects on viability and neuronal differentiation. Viability straight after harvesting of differentiated cells was above 90% as indicated by the trypan blue exclusion test. This good viability was maintained after re-seeding these cells for 24 h (Figure
Figure 1
Viability and neuronal phenotype of re-seeded cultures
Viability and neuronal phenotype of re-seeded cultures. Harvesting and re-seeding of long-term differentiated cultures did not significantly affect their viability (A, Live/Dead stain), neuronal differentiation (B,β-III-tubulin, Tuj), DARPP-32 (C), or astrocytic differentiation (D).
Cell implants do not impact on weight loss
Body weight is a reliable indicator of the overall health of R6/2 mice. The body weight of wild type (WT) and R6/2 mice (n = 10/genotype) steadily increased until 8 weeks of age (1 week post-implantation, Figure
Figure 2
Body weight
Body weight. Weight gain between groups was equivalent up to 7 weeks of age when animals were grafted. Post-implantation WT mice with vehicle injection continued to gain weight. All R6/2 mice started to lose weight 3 weeks post-grafting (11 weeks of age). There was no effect of the implantation of undifferentiated or differentiated cells on body weight.
Neither undifferentiated, nor differentiated cells improve behavioural deficits
The development of a progressive behavioural phenotype is a key characteristic of R6/2 mice. Up to 8 weeks of age (1 week post-implantation), the R6/2 animals performed as well as WT controls on the rotarod (Figure
Figure 3
Behaviour.A. Rotarod: no effect of genotype on rotarod performance was detected one week pre- or post-grafting. However, a significant impairment was evident in R6/2 mice at 3 and 5 weeks post-grafting which was not improved through the implantation of undifferentiated or differentiated cells. B. Open field: the total exploratory activity of R6/2 was reduced compared to WT controls at all time points tested. Neither undifferentiated, nor differentiated, cells attenuated the deterioration of R6/2 exploratory behaviour. C. Grip strength: all R6/2 mice showed significantly impaired performance compared to WT mice at all time points. No beneficial effect of treatment was evident. (* p < 0.05, **p < 0.01, and ***p < 0.001).
Cell implants did not reduce brain atrophy
To determine whether cell implants reduced the brain atrophy of R6/2 mice compared to WT controls, T2-weighted MRI scans were acquired 6 weeks post-grafting (13 weeks of age). R6/2 mice injected with vehicle, undifferentiated or differentiated exhibited similar levels of atrophy in the striatum (Figure
Figure 4
MRI. Volumetric analysis revealed a significant atrophy in striatal (A), cortical (B), and hippocampal tissue (C). Lateral ventricles (D) were minimally enlarged, but no significant statistical effect was evident. There was no treatment effect of the implantation of either undifferentiated or differentiated cells. (* p < 0.05, **p < 0.01, and ***p < 0.001).
Survival and differentiation of cell implants
The survival and differentiation of the implanted cells are essential to guarantee a potentially beneficial effect. Post-mortem immunohistochemical analyses 6 weeks post-implantation indicated that in 70% of R6/2-undiff and 50% of R6/2-diff animals some STROC05 cells survived six weeks post-implantation. A re-analysis of the behaviour and MRI results indicated that exclusion of animals without surviving cells did not significantly affect outcome (Additional file
Additional file 4
Figure S4. Re-analysis of the main outcome measures. As some animals had no graft survival, it is conceivable that this would affect the group outcome measure. Therefore we reanalysed the data excluding these animals. The analysis containing all animals is presented on the left and the reanalysed data on the right. Exclusion of animals without graft survival, however, did not make a difference to these results.
Click here for file
Figure 5
Survival of the transplanted cells
Survival of the transplanted cells. A small population of implanted cells survived (A). Human cells (human nuclear antigen + cells in pink) were mostly found within the injection tract in the striatum. A select few individual cells were observed migrating along the corpus callosum. Stereological cell counts revealed no significant difference between undifferentiated and differentiated cell implantation (B). However, cell survival was very variable with some animals having no surviving cells. Neuronal differentiation, as determined by FOX3 staining, of implanted cells was very poor (C&D). Most implanted cells differentiated into astrocytes (E), whereas others did neither express markers of neurons nor astrocytes. (Scale bar 200 μM).
Cell therapy for Huntington's disease is potentially an important intervention to delay, stabilize and/or improve impairments. These therapeutic effects are well documented in animal models, but more limited, albeit positive, evidence is available in patients with Huntington's disease that received fetal tissue transplants
Lack of efficacy and poor cell survival
Therapeutic efficacy in Huntington's disease is considered to be associated with a decrease in neurodegeneration, as well as a replacement of lost striatal DARPP-32+ GABAergic output neurons. An intra-striatal injection of fetal-derived neural progenitors/stem cells
It is therefore important to consider why STROC05 cells did not improve outcome. Foremost of all, survival of cells after implantation was rather poor with only 161 human cells surviving in one hemisphere. Although there have been reports of behavioural changes with 124 cells surviving in stroke
Although the survival of cells is thought to be essential to establish recovery by means of intracerebral hNSC implantation, the lack of differentiation of STROC05 cells might also preclude recovery. Especially, the differentiation of cells into striatal DARPP-32+ GABAergic output neurons has been considered to be directly linked to the degree of functional recovery
Apart from poor cell survival and differentiation, it is plausible that, even if these issues are overcome, this cell line is not efficacious in Huntington's disease. If this would indeed be the case, this cell line would provide an indispensable "therapeutic control" condition against which mechanisms of efficacious cells could be compared. Nevertheless, it is also conceivable that this cell line could provide efficacious results if implanted under different experimental conditions.
Choosing an appropriate animal model of Huntington's disease
STROC05 cells might be efficacious for Huntington's disease, but it is possible that testing them in the R6/2 model does not reflect their therapeutic potential. The R6/2 model rapidly manifests behavioural impairments, as well as regional brain atrophy. This rapid progression of disease might be appropriate for screening pharmacological agents that exert immediate effects, but the time window might be too short and aggressive to evaluate the efficacy of neural progenitor/stem cells. Mouse models that develop neuronal loss over a protracted time course, such as the YAC72
A combination of treatments for various aspects of the disease might be needed for implanted cells to be efficacious. For instance, an injection of only mouse neural progenitors did not maintain motor function in N171-82Q transgenic mice, but if these same progenitors were engineered to also secrete GDNF, they provided a therapeutic benefit
Neither the implantation of undifferentiated, nor pre-differentiated human NSCs promoted behavioural benefits or attenuated the on-going neurodegenerative process. This is likely due to a combination of factors, most importantly cell survival was insufficient to impact on the progression of the disease, but the life-span of R6/2 mice might also be too short to appropriately evaluate neural progenitors/stem cells. More chronic transgenic models are likely to be better in evaluating these therapies. However, implantation of cells by themselves is unlikely to be sufficiently efficacious to promote recovery, but rather a combination of multiple treatments will be required to provide a truly efficacious therapy that can impact on the clinical condition.
Competing interests
MM previously received financial and personnel support from ReNeuron Ltd to study the efficacy of a hNSC line in a rat model of stroke.
Authors' contributions
GEA: design of study, in vitro experiments, behavioural testing, magnetic resonance imaging, stereotactic surgery, post-mortem histology, statistical analyses, first draft of manuscript; IR: design of study, stereotactic surgery, magnetic resonance imaging; revising of manuscript; SG: conducted the 3-NPA study; RG: breeding and genotyping of animals; GB: conception of study, financial support, revising of manuscript; MM: conception and design of study; financial support; statistical analyses; drafting of manuscript; final approval of manuscript. All authors read and approved the final manuscript.
This study was funded through a translational stem cell grant by the UK Medical Research Council (G0802552 & G0800846) and a PhD studentship by the Egyptian Government (MM45/07). The authors would like to thank Dr Anthony Vernon for assistance with the 3-NPA study, as well as Dr Po-Wah So, the manager of the preclinical imaging unit at KCL, and the British Heart Foundation who funded the MRI scanner.
Therapeutic Advances in the Management of Huntington's Disease
Human neural stem cell transplants improve motor function in a rat model of Huntington's disease
Histological findings on fetal striatal grafts in a Huntington's disease patient early after transplantation
Neuropsychological functioning following fetal striatal transplantation in Huntington's chorea: three case presentations
Conditionally immortalized, multipotential and multifunctional neural stem cell lines as an approach to clinical transplantation
Making stem cell lines suitable for transplantation
Pharmacological MRI of stem cell transplants in the 3-nitropropionic acid-damaged striatum
Preservation of striatal tissue and behavioral function after neural stem cell transplantation in a rat model of Huntington's disease
Induction of GABAergic phenotype in a neural stem cell line for transplantation in an excitotoxic model of Huntington's disease
Survival, neuronal differentiation, and fiber outgrowth of propagated human neural precursor grafts in an animal model of Huntington's disease
Human fetal cortical and striatal neural stem cells generate region-specific neurons in vitro and differentiate extensively to neurons after intrastriatal transplantation in neonatal rats
Volume and differentiation of striatal grafts in rats: relationship to the number of cells implanted
The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain. II. Correlation between positron emission tomography and reaching behaviour
The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain. I. Morphological characteristics
Transplanted hNT cells ("LBS neurons") in a rat model of Huntington's disease: good survival, incomplete differentiation, and limited functional recovery
Medium spiny neurons for transplantation in Huntington's disease
GABAergic Neurons from Mouse Embryonic Stem Cells Possess Functional Properties of Striatal Neurons In Vitro, and Develop into Striatal Neurons In Vivo in a Mouse Model of Huntington's Disease
Striatal progenitors derived from human ES cells mature into DARPP32 neurons in vitro and in quinolinic acid-lesioned rats
Effect of inflammatory cytokines on major histocompatibility complex expression and differentiation of human neural stem/progenitor cells
Purmorphamine Increases DARPP-32 Differentiation in Human Striatal Neural Stem Cells Through the Hedgehog Pathway
The R6 lines of transgenic mice: a model for screening new therapies for Huntington's disease
A conditionally immortal clonal stem cell line from human cortical neuroepithelium for the treatment of ischemic stroke
Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain
Neural differentiation from human embryonic stem cells in a defined adherent culture condition
Differentiation of human embryonic stem cells to regional specific neural precursors in chemically defined medium conditions
Standardization and statistical approaches to therapeutic trials in the R6/2 mouse
Identical oligomeric and fibrillar structures captured from the brains of R6/2 and knock-in mouse models of Huntington's disease
Chronology of behavioral symptoms and neuropathological sequela in R6/2 Huntington's disease transgenic mice
Longitudinal characterization of brain atrophy of a Huntington's disease mouse model by automated morphological analyses of magnetic resonance images
Microglia density decreases with age in a mouse model of Huntington's disease
Convection-enhanced delivery in the treatment of epilepsy
Characterization of progressive motor deficits in mice transgenic for the human Huntington's disease mutation
Hsp27 overexpression in the R6/2 mouse model of Huntington's disease: chronic neurodegeneration does not induce Hsp27 activation
Combination therapy using minocycline and coenzyme Q10 in R6/2 transgenic Huntington's disease mice
The mTOR kinase inhibitor Everolimus decreases S6 kinase phosphorylation but fails to reduce mutant huntingtin levels in brain and is not neuroprotective in the R6/2 mouse model of Huntington's disease
Striatal expression of a calmodulin fragment improved motor function, weight loss, and neuropathology in the R6/2 mouse model of Huntington's disease
The mTOR kinase inhibitor Everolimus decreases S6 kinase phosphorylation but fails to reduce mutant huntingtin levels in brain and is not neuroprotective in the R6/2 mouse model of Huntington's disease
Assessment of motor coordination and balance in mice using the rotarod, elevated bridge, and footprint test
Mitochondrial calcium uptake capacity as a therapeutic target in the R6/2 mouse model of Huntington's disease
Brief ampakine treatments slow the progression of Huntington's disease phenotypes in R6/2 mice
Inhibition of the striatal specific phosphodiesterase PDE10A ameliorates striatal and cortical pathology in R6/2 mouse model of Huntington's disease
Comprehensive behavioral testing in the R6/2 mouse model of Huntington's disease shows no benefit from CoQ10 or minocycline
Cell-based treatments for huntington's disease
Mesenchymal stem cell transplantation and DMEM administration in a 3NP rat model of Huntington's disease: morphological and behavioral outcomes
Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model
Neural transplantation of human neuroteratocarcinoma (hNT) neurons into ischemic rats. A quantitative dose–response analysis of cell survival and behavioral recovery
DARPP-32-rich zones in grafts of lateral ganglionic eminence govern the extent of functional recovery in skilled paw reaching in an animal model of Huntington's disease
Stem cells: prospects for functional repair of brain damage
Tenascin-R promotes neuronal differentiation of embryonic stem cells and recruitment of host-derived neural precursor cells after excitotoxic lesion of the mouse striatum
Death of dopaminergic neurons in vitro and in nigral grafts: reevaluating the role of caspase activation
Pharmacologically active microcarriers releasing glial cell line - derived neurotrophic factor: survival and differentiation of embryonic dopaminergic neurons after grafting in hemiparkinsonian rats
In vivo evaluation of pharmacologically active microcarriers releasing nerve growth factor and conveying PC12 cells
A YAC mouse model for Huntington's disease with full-length mutant huntingtin, cytoplasmic toxicity, and selective striatal neurodegeneration
Neurological abnormalities in a knock-in mouse model of Huntington's disease
Neuronal degeneration in striatal transplants and Huntington's disease: potential mechanisms and clinical implications
Striatal transplantation in a transgenic mouse model of Huntington's disease
Human mesenchymal stem cells prolong survival and ameliorate motor deficit through trophic support in Huntington's disease mouse models.
Ex vivo delivery of GDNF maintains motor function and prevents neuronal loss in a transgenic mouse model of Huntington's disease
Intracerebral transplantation of neural stem cells combined with trehalose ingestion alleviates pathology in a mouse model of Huntington's disease
A pharmacological MRI assessment of dizocilpine (MK-801) in the 3-nitroproprionic acid-lesioned rat


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Kanye West protégé Rhymefest has purchased The Life of Pablo rapper's childhood home in Chicago with plans to convert the location into a "community arts incubator" and the permanent home of West's Donda's House organization.
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New Delhi: The Indian cricket board's exemplary punishment for four players involved in the match-fixing scandal has robbed them of a chance to attain several personal milestones in Tests as well as One-day Internationals. Although the Board of Control for Cricket in India (BCCI) has banned the four - Mohammed Azharuddin, Manoj Prabhakar, Ajay Sharma and Ajay Jadeja - for different periods, pending appeal, only Jadeja has a realistic chance of returning to the national team. All players have said they would appeal to the Board against the penalty. Wicket-keeper Nayan Mongia, who has been exonerated, is unlikely to be picked again in the national team. He has, however, vowed to stage a comeback. Former Indian team physiotherapist Ali Irani has also been banished for five years along with Prabhakar and Jadeja. Former Indian captain Azharuddin, who has been banned for life, will be disappointed at failing to reach the 100-Test figure. Unless his ban is reduced - as some board officials are demanding - the 37-year-old batsman is certain to remain stranded at 99 Tests. Azharuddin can claim to be unlucky for missing out on 100 Tests. The stylish batsman from Hyderabad would have reached the three-figure mark had he not got injured on the eve of the first Test against South Africa in Mumbai in February. At that stage, he was returning to the Indian team after having been dropped from the preceding Australian tour. Azharuddin finally returned, in the second Test in Bangalore, and cracked a century (102) in the second innings, which has turned out to be his last Test innings. Azharuddin set another landmark in his last innings, albeit by default. He became the second batsman in the 123-year-old history of Test cricket, after Australian Greg Chappell, to score a century in the first and last innings of his career. Azharuddin remains India's most successful Test captain with 14 wins. He led the country in 47 Tests, same as the second most-successful captain Sunil Gavaskar. He lost 14 Tests and drew 19 while Gavaskar drew a world record 30 matches. A product of Hyderabad's All Saints School and Nizam College, Azharuddin's Test-run aggregate of 6,215 is the third highest in India, after Gavaskar and Dilip Vengsarkar. However, during his 147 innings, Azharuddin never made a double century, his highest being 199, scored against Sri Lanka in Kanpur in 1986. Azharuddin will also fall short of another important mark in a batsman's Test career, an average of 50, as he will remain stuck at 45.03. He will also rue missing the 10,000-run mark in One-dayers, his tally reading 9,378 in 334 matches. Azhar has held 105 catches in Test cricket. If 29-year-old Jadeja does not return to the Indian team, his highest Test score of 96 will always haunt him. The bubbly all-rounder was run out four short of his maiden Test ton while opening the innings against the West Indies at St. John's, Antigua, in 1997. Although Jadeja was never considered fit for Test cricket, given his batting style, he played 15 matches, scoring 576 runs at 26.18 and hitting four half centuries. In One-dayers, Jadeja did much better scoring 5,355 runs at an average of 37.44, with six centuries to boot. However, he will not be able to join the club of cricketers to have played 200 One-day internationals, having played 196 matches. Jadeja, who starred in several Indian victories in the shorter version of the game, also led India in 13 matches, winning eight and losing five. Mongia, the 30-year-old stumper from Baroda, fancies his chances to stage a comeback. Presuming Mongia fails to return, he will be left at 94 catches, though he has effected seven stumpings, making it 100-plus dismissals behind the wickets. He has taken 110 catches in One-dayers. Mongia will also regret the fact that he never scored a One-day century. Ajay Sharma, who last played for India in 1993, had only domestic tournaments to look forward to. Sharma, however, holds an enviable record - he has the all-time third-best batting average in first-class cricket of those scoring 10,000 or more runs, behind Australian Sir Don Bradman and Vijay Merchant of India. India Abroad News Service
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Original Article
Designing Service Level Agreements for Inventory Management
Version of Record online: 1 JUL 2013
DOI: 10.1111/poms.12033
© 2013 Production and Operations Management Society
Production and Operations Management
Volume 22, Issue 5, pages 1103–1117, September–October 2013
Additional Information
How to Cite
Liang, L. and Atkins, D. (2013), Designing Service Level Agreements for Inventory Management. Production and Operations Management, 22: 1103–1117. doi: 10.1111/poms.12033
Publication History
- Issue online: 16 SEP 2013
- Version of Record online: 1 JUL 2013
- Manuscript Accepted: JUL 2012
- Manuscript Received: NOV 2010
Funded by
- Research and Postgraduate Studies Committee of Lingnan University. Grant Number: DR10C2
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Was today's event a bought-and-paid-for publicity stunt to increase the next Conservative candidate's profile in new Barrie-Oro-Springwater (Midhurst) riding with the first federal election being in Oct 2015? Did money donated to the Midhurst Ratepayers' Association, MRA and Environmental Defence Canada, EDC go toward the cost of this event or was the money from another source? Are either or both of them shills for a political party that represents pro-development or can any interest group buy their brand? I recall the MRA accusing the Township of Springwater for calling short notice and scheduling information sessions at unbelievably difficult times for citizens to attend. They then said there were "extensive good faith consultations". Who paid for the show today?
1. Extremely Short Notice: I received the bulk email from the MRA last night 11:18 pm (Dec 6th). That would have given me 13 hours to re-arrange my Saturday afternoon. Oddly, the EDC media advisory also had a very short media fuse as well: out Dec 6th.
And, to boot, the ED communique mentioned a federal MP (Mr. Patrick Brown Barrie) would be attending although (1) Midhurst is in the Simcoe-Grey riding (Hon. Dr. Kellie Leitch MP) and (2) growth and sprawl are a provincial not a federal jurisdiction. Shouldn't Jim Wilson MPP Simcoe-Grey who is the 2nd most powerful Conservative in the Hudak caucus (or even Rod Jackson), be explaining growth issues to his own constituents?
I would have gone to it because I would have loved to ask some questions of David Strachan, president of the Midhurst Ratepayers', Alex Nuttall, City of Barrie Ward 10 councillor (farthest south and east ward in Barrie, rumoured MP hopeful) and EDC. I believe I am a member in good standing and have contributed financially to both the MRA and EDC. 2. Missing Article: In fact, I wrote the only article about the Midhurst Secondary Plan for EDC at the request of their Claire Malcolmson. It was called Midhurst Secondary Plan: Village to grow 10 times? and was published on their weblog on February 13, 2012. When I tried clicking through on the URL that I had listed as a pdf this is the image that came up:
So as an author I no longer have access to the article? I also tried searching ED's blog with the title and my name, but no luck. Click here for a pdf copy of the article or here for a post that I wrote called: Land speculation and other confidence games have always relied on human weakness., iLoveMidhurst.ca, February 14, 2012. 3. ED's legal counsel is listed as David Donnelly.
He was Executive Director of Environmental Defence Canada from 1988 to 1996 in addition to having been very involved in the Big Bay Point SLAPP situation as well as many ongoing First Nations issues.
The only federal issue that I am aware of that links Mrs. Brown, Strachan and Donnelly are the ladies at Springwater Park – Camp Nibi Cross-posted on SpringwaterParkcc.org.
Les Stewart Gets Results!!
Dec 11, 2013 Update: To give credit where credit is due, Mr. Aidan Grove-White, Environmental Defence Canada, EDC contacted me directly and we had a very productive conversation. He promised to look into the misplaced weblog article and we discussed the lessons I've learned about being the subject of two SLAPPs (1, 2, 3, 4, 5)and the reason I resigned as president of the Midhurst Ratepayers' Association (before Dr. Paul Fleming and the current Mr. David Strachan), and other local matters. Full marks for transparency and accountability to EDC and internet monitoring.
This is the only response to this specific post or these two related ones:


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Greenville Medical Association can be found at E Morgan St 175. The following is offered: Trade Organizations. The entry is present with us since Sep 8, 2010 and was last updated on Nov 12, 2013. In Georgiana there are 1 other Trade Organizations. An overview can be found here.
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Pear trees were medium-sized fruit trees which could be found in the southern woodlands of Rokugan. [1] The fruit was edible, and it could be used to brew a brandy. [2] A famous pear wine was made in Zakyo Toshi, in the One Tree Brewery. [3]
|This page uses content from Wikipedia. The original article was at Pear. The list of authors can be seen in the page history. As with L5r: Legend of the Five Rings Wiki, the text of Wikipedia is available under the GNU Free Documentation License.
|This article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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Servicing Kangaroo Valley, Kiama Heights, Kiama Downs and 17 other suburb(s)
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This business can travel to you.
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70 Central Ave, Oak Flats, NSW 2529
1/26 Waitangi Street, Gwynneville, NSW 2500
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13 Toolangi Cl, Cordeaux Heights, NSW 2526


text:
It's a privilege and an honor to be trusted with this responsibility. No, we're not talking about heart surgery with Don Draper, but about choosing the fourth annual All-Giant Killers Team. This year, after we opened the floor to nominations for players who contributed the most to deep underdogs in the NCAA tournament, we got more -- and more intense -- responses than ever before.
So we want to start with a heartfelt thanks for all of your feedback. We are proud that Giant Killers has loyal readers. We are including the best of the GK Mailbag here, with the 2013 All-GK Team. And we're happy to incorporate your suggestions as we keep improving our statistical model.
Now, longtime readers may remember that in 2011, we awarded naming rights to this crew. No, not in exchange for cash, but to honor the first Killers to knock off Giants in consecutive years: the UAB Blazers, who slayed Kentucky in a 9-1 matchup in 2004, then came back as an 11-seed and beat LSU, a No. 6, in 2005. (VCU in 2011 and 2012 is the only other Killer with victories over Giants in back-to-back seasons.)
Coached by Mike Anderson (now at Arkansas) and led by twins named Donell and Ronell Taylor (who now play in Italy and Belgium, respectively), those UAB squads had a crucial element of the GK formula down cold: there was a massive gap of nearly 10 percentage points between how often they forced and committed turnovers. Here they are in action, with Ronell grabbing a steal and hurling a two-handed, overhead and, oh yeah, backward pass to Donell. Just like Brenner to Keating.
Hence, the 2013 Donell and Ronell All-Giant Killers Team:
Sherwood Brown, G, Florida Gulf Coast Eagles
All he did was deliver an inspirational pre-game speech, drop 24 points on Georgetown while hitting 3-of-6 from downtown and adding 9 rebounds, shake hands with the CBS broadcast crew, celebrate with cheerleaders -- and then rinsed and repeated against San Diego State. Signature player on a memorable underdog.
Reader comment, from TheChewmiester: "[Brett] Comer and Brown from FGCU should be at the top of the GK team. If they are not, the list will be invalid."
Our response: We appreciate it any time someone's willing to pick a fight over the All-GK team. But there are a lot of possibilities this year, and we're trying to limit programs to a maximum of one representative. Apologies to Comer -- and to Bernard Thompson.
Andre Hollins, G, Minnesota Golden Gophers
He helped validate stathead claims that Minnesota was actually the better team in their 11-6 matchup against UCLA by scoring 28 points against the Bruins. Then he added 25 against Florida, including three 3-pointers in an 11-2 run that almost, but not quite, brought the Gophers back against the Gators.
Reader Comment, from Ssilvi84: "I gotta give a nod to Dre Hollins of Minnesota. He torched UCLA and was the catalyst in bringing MN back from down 23 to [9]. He picked up a horribly ticky-tack foul, his fourth, and the Gophers went ice-cold while he was on the bench. He stays in the game, we might not be talking about Tubby Smith being fired."
Cleanthony Early, F, Wichita State Shockers
Grabbed 38 rebounds in the tournament, including 14 off the offensive glass and came off the bench to key the Shockers' dramatic upset of No. 1 seed Gonzaga. Of course, we could name Malcolm Armstead, Wichita State's leader, here, or
Carl Hall, whose shot blocking was the Shockers' secret weapon, or even Fred VanVleet, whose increased playing time helped elevate the team's play to another level as Wichita State made one of the all-time great Giant-Killing runs.
Reader comment, from ashatton: "Armstead ... Tough call over teammates Early and Hall, but ultimately he's their go-to guy."
Our response: Yes, but Armstead shot 31.9 percent in the tournament (22 of 69), and Early shot 50 percent (30 of 60). Giant Killers are supposed to be efficient.
Arsalan Kazemi, F, Oregon Ducks
How do you beat a team seeded seven spots higher when shoot just 39 percent and turn the ball over more than your opponent? You need lots of extra possessions, and Kazemi had more offensive rebounds than the entire Oklahoma State team in their Round of 64 matchup. Then he had more than twice as many as St. Louis, combined, in the Round of 32. And 11 points (on 5-for-6 shooting), 12 boards and no turnovers against Louisville to cap his college career was pretty good, too.
Tyrone Garland, G, La Salle Explorers
In a season without Wichita State or FGCU, we'd all be marveling over La Salle, a smart, efficient 13-seed that found two key ways to eke out three tournament wins, including a killing of vulnerable Giant Kansas State. They protected the ball (turnovers on just 15.7 percent of possessions in the Big Dance) and hit bombs (34-of-80 on 3-point attempts, or 42.5 percent).
And after Garland scorched Boise State, Jerrell Wright stepped up against K-State and Ramon Galloway had a monster game against Ole Miss. So consider this a team pick, which we're giving to Garland because of his game-winning shot against Mississippi -- the Southwest Philly Floater that launched a legend, not to mention a frenzy of local news stories and t-shirts.
Honorable Mention: Laurent Rivard, G, Harvard Crimson
Rivard's 17 points (on 5-for-9 3-point shooting), 5 rebounds and 2 steals in 40 minutes not only helped Harvard upend New Mexico, they earned Rivard the first-ever support for an Ivy League player in all-GK balloting. How big was the Crimson's win over the Lobos, the first ever in the NCAA tournament for Harvard? It was covered on PBS. And how cool is Rivard, a native of Quebec and a computer science major? So cool he can make up his own mysterious gesture on the court.
Although we have to note: This Harvard Crimson profile says that as a sophomore, Rivard was living in Cabot House, a former Radcliffe dorm in the nether reaches of campus, while his university bio places him at Eliot House, a bastion of old-money privilege. Is Rivard already selling out? Say it ain't so, Laurent!
Top Mongoose:
Last year we instituted this award for the top Giant Killer-killer. After all, if Killers are akin to snakes, sneaking around, waiting to strike down Giants, players who can stomp them out are like the mongoose. This time around, the prize goes to Victor Oladipo.
After Temple outplayed Indiana for about 58 minutes in the second round, Oladipo grabbed a rebound and hit a free throw to give the Hoosiers a 53-52 lead. After another rebound, he then sank a 3-point bomb with 15 seconds left in the game, killing the Owls' dreams of an upset -- and Khalif Wyatt's chances of making the All-GK team.
Finally, we'll conclude with a comment from reader Kingofboggle: "Your all-time GK team needs to include Ohio's D.J. Cooper. Led OU as a No. 14 seed over Georgetown in 2010. And then led the Bobcats to the Sweet 16 in 2012 as a No. 13, winning over Michigan and South Florida before falling to UNC in OT. He embodies the guard play that all GKs have to have. And he did it twice."
Well put, King -- which is why we loved Cooper, put him on the All-Giant Killers team last year and gave him an honorable mention in 2010. But honestly, until you brought it up, we hadn't ever thought about an all-time All-GK team.
We just got ourselves some homework for the summer.


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Paneer Biryani
READY IN: 1hr 20mins
Recipe by roja khan
serve with raita and salad
See Discussion:
Ingredients Nutrition
- 200 g cottage cheese (Paneer Cubes)
- 1 1⁄4 cups basmati rice
- 2 medium onions
- 1 inch ginger
- 5 garlic cloves
- 1⁄4 cup coriander leaves
- 1⁄4 cup mint leaf
- 2 medium tomatoes
- 1⁄2 cup skim milk yogurt
- 4 -5 saffron strands
- 1 bay leaf
- 4 cloves
- 2 green cardamoms
- 2 black cardamom pods
- 1 inch cinnamon stick
- salt
- 2 tablespoons oil
- 1 teaspoon red chili powder
- 1 teaspoon coriander powder
- 1⁄2 tablespoon peppercorn (crushed)
- 1⁄2 teaspoon cumin powder
- 1⁄2 teaspoon turmeric powder
- 1⁄2 teaspoon garam masala powder
- 4 -5 drops kewra essence
Directions See How It's Made
- Pick and wash basmati rice in plenty of water and soak in sufficient water for half an hour.
- Peel and finely slice onions. Peel ginger, garlic and grind together to a fine paste. Clean, wash and finely chop fresh coriander and mint leaves.
- Wash tomatoes and make a puree in a blender. Whisk the skimmed milk yogurt and keep aside. Soak the saffron in one-fourth cup warm water.
- Boil three to four cups water in a thick-bottomed vessel, add bay leaf, cloves, green cardamoms, black cardamoms, cinnamon, mace and one teaspoon salt. When the water starts boiling rapidly, drain the soaked basmati rice and add.
- Cook for eight to ten minutes, stirring frequently or until the rice is three fourth done. Drain in a colander.
- Heat oil in a non-stick pan, add sliced onions and stir-fry over high heat for two to three minutes or until the onion turns translucent. Add ginger-garlic paste and cook briefly.
- Add red chilli powder, coriander powder, crushed peppercorn, cumin powder and turmeric powder. Stir-fry briefly and add the pureed tomatoes.
- Continue cooking over high heat for another two to three minutes, stirring continuously or until masala is fairly thick.
- Add the whisked skimmed milk yogurt, garam masala powder and half the quantity of chopped fresh coriander and mint leaves. Stir well and cook for two minutes more.
- Add the paneer and salt to taste. Stir-fry over high heat for two to three minutes and remove from heat.
- Arrange the cooked rice and paneer masala in alternate layers in an oven proof dish (or biryani handi), sprinkling the chopped fresh coriander and mint leaves, kewra water and the water with saffron after each layer. Ensure that the top most layer is of rice.
- Cover the assembled biryani with a tight-fitting lid and seal the edges with kneaded atta dough (if required).
- Keep the sealed dish on a tawa and cook on low heat for ten to fifteen minutes. You can also place a few burning charcoals on the lid. Alternately, cook in a pre-heated oven at moderate temperature for ten to fifteen minutes.
- Break open the seal and serve biryani immediately.


text: Nathan and Heather Sanborn from Rising Tide Brewing Company will be joined by Will and Kathleen Pratt from Tandem Coffee Roasters to celebrate the release of Waypoint, a new coffee porter brewed as a collaboration between these two East Bayside neighbors. Join us for Waypoint with a coffee back (10 oz. of beer plus 2 oz. of coffee on the side!) and special deals on Tandem's Waypoint Blend beans combined with a four-pack of Rising Tide's Waypoint beer. Meet the creators of this new beer and celebrate neighborhood collaboration! Check out the facebook event here.


text:
IN growing the bank businesses, Wema Bank said, it is set to complete a $100 million Tier II capital raising exercise by the second quarter of this year.
The managing director of the bank, Mr. Segun Oloketuyi said this at the Bank's fact behind its figure on the Nigerian Stock Exchange, NSE.
Oloketuyi said that the company is about to finalise tier 2 capital raise of $100 million to increase its capital position.
He added that the company had moved from negative capital position of N45 billion in 2009 to sufficient capital of N44 billion in shareholders' funds, saying that in the period the bank moved from a distressed bank to a fully capitalised CAR of 18 per cent.
According to him, the bank will continue to implement it project LEAP in growing, low cost funding, efficiency, alternative platforms and performance.
Explaining further, Oloketuyi said, the bank will improve growth in retail deposits to keep cost of funds low and leverage on mobile solutions and technology as its competitive edge.
He pointed out that the bank aimed to drive better operating margins, achieve improved ROEs and grow foreign partnerships and syndications.
He noted that the bank would not be able to pay dividend until the retain earning move from negative to positive, adding that very soon the bank would reward its shareholders.
He added that the funds raised from the tier 2 market will enable the bank to achieve all this as it will have enough capital to work with.
Also, speaking the chief financial officer of the bank, Tunde Mabawonku said this offer will make the Bank's capital ratio to top 25 percent next year, after the capital raising exercise from 18 percent now.
Speaking on the bank 2014 financial performance, he said gross earnings increased by 19.90 per cent between 2013 and 2014, saying that its profit before tax grew by 58.88 per cent to N3.1 billion from N1.9 billion in 2013.
He added that deposit from customers increased by 17 per cent while deposit from retail and commercial segment dominated total deposit in the year under review.
He pointed out the bank has continued to diversify its loan portfolio as the total loans as at 2014 was N149 billion, while non- performing loans was N3.79 billion as a result of aggressive loan recovery.
IN growing the bank businesses, Wema Bank said, it is set to complete a $100 million Tier II capital raising exercise by the second quarter of this year.


text:
For decades, the U.S. Navy has been built around its carrier battlegroups and amphibious ready groups: huge clusters of heavily armed warships, tailored for delivering massive firepower, in the form of airplanes or Marines. But for today's "hybrid" wars, where winning over local populations is as important as killing the bad guys, these traditional naval groups aren't necessarily the best things.
The idea is that instead of having a group of ships centered on an aircraft carrier, whose primary mission is to launch strikes on shore, we ought to have groups of ships that have as primary functions training, providing humanitarian disaster relief, and U.S. smart power in Latin America supporting humanitarian projects. What I am thinking about specifically is centering a group around a hospital ship and then including in that group several smaller ships that bring training capability with them.
Stavridis, widely considered the Navy's answer to the Army's Gen. David Petraeus, is no stranger to new concepts. During his stint at Southcom, he deployed the Navy's new catamaran high-speed transports, including the super-speedy Stiletto. He even blogs!
There's evidence the Navy might take Stavridis' idea seriously. The sea service has heavily emphasized humanitarian missions, of late. Plus, Navy Cmdr. Jerry Hendrix is helping craft the Navy's portion of the Quadrennial Defense Review. In a recent article, Hendrix proposed mixing catamarans, Stilettos and Littoral Combat Ships, to form "Influence Squadrons," optimized for humanitarian and training missions.
[PHOTO: Southcom]
Read the original article on WIRED.COM


text:
A/N: Hey, I came up with this idea a while ago, and haven't managed to get it out of my head. There will be more to come, but I don't know when or how long it'll be.
Warning: Contains Rape and M-preg. If you don't like this then please don't read it.
Harry slowly opened his eyes, unsure of where he was. He blinked a few times to try and clear his vision, and the bright antiseptic light of the hospital wing came into vision. His hand reached to his face, feeling for his glasses, not finding them. He reached blindly for the cabinet at the side, feeling for them, and positioned them on his face. Once he could see properly he looked around himself and his vision came to rest on Hermione sat next to his bedside, her normally calm face, troubled and flushed, streaked with dry tears.
"Her-Hermione." He rasped his throat feeling like he'd been swallowing razorblades. He leant over and brushed her knee with his fingertips. The contact woke her, and she jumped, startled.
"Harry," She exclaimed "You're awake!" a single tear ran down her face and she looked away, lifting a slender hand to hastily brush it away.
"What's wrong?" he questioned quietly. She looked back at him, her eyes searching his drained face.
"I don't know Harry. I don't know how I should be feeling. Happy or Mad, Glad or Sad." She took a deep breath.
"Why Harry? Why didn't you tell me?" Her eyes implored him to give her an answer, but he didn't have the slightest idea what she was talking about. What had so upset her?
"Tell you? Tell you what?" Harry was really confused. What was he meant to have told her, and why was he in the hospital wing?
"You, you let me..." she broke of with a sob, and put her head in her hands. "You let me fall in love with you, when all the time….." She continued, looking back up at him. Harry's brain only caught one important fact from her words.
"You love me?" he whispered reverentially. A ghost of a smile graced his face. Hermione loved him. His insides were dancing for joy. For so long now he'd been falling in love with his best friend, unsure of her feelings. He'd kept it inside, but they'd become closer, spending an increasing amount of time alone together, a thing that kept Harry very happy. It kept Ron quite happy too, his Honeydukes supply had never looked so healthy with all the times Harry had offered him sweets to find an elsewhere to be.
"Yes Harry, I did. All this time I loved you and I thought – I thought that you might have felt something for me too. But you were just being cruel, leading me on. You should have told me Harry, not just played with my feelings like this. Its, it was – hurtful and cruel. And I wouldn't have believed it of you." She broke of again, her breathing heavy with her anger and hurt.
"But, I do feel something for you 'Mione, I Lo..." She cut him off.
"No Harry. You don't feel anything. If you felt even the slightest affection for me, you'd have told me. And I'd have been fine with it. But to pretend like you did. What am I to you? Just some kind of, of plaything. A toy to mess around with whenever you feel like it? Well I'm not Harry. I won't let you do it anymore." She jumped out of her seat, but Harry's hand grabbed her wrist before she could leave.
"Hermione, I don't have a clue what you're talking about. What have I done to upset you? Why am I even in the hospital wing? And you're wrong. I do feel for you. I love you. And I would never deliberately hurt you. Never"
Hermione started to say something, only to be cut off by the sound of the door opening, and two people entering the room.
"Ahh, Mr Potter, I see you have woken up. You gave us quite a scare." Dumbledore's voice was light as it usually was.
"Sir, I'm a little confused. Why am I here?" Dumbledore carefully studied Harry's face before seeming to come to a decision.
"Well, Harry. You gave us a bit of a scare this morning at breakfast, when you stood to leave and then suddenly collapsed." Madame Pomfrey started fussing over him, bending over him and adjusting his pillows, feeling his forehead and taking his pulse.
"Yes Harry. Have you been feeling ill at all lately?" Harry thought about the headmaster's question.
"Uh, yes I have felt a bit off. And I've been throwing up for the last week too. I thought I'd just got a virus, but to pass out, I guess it must be worse than I thought. And for Hermione to be so upset… What's wrong with me?"
"Harry, nothing is wrong exactly. But something is not quite right." Dumbledore was as cryptic as ever, and his comment left Harry more in the dark than before. He looked from the headmaster's face to Madame Pomfrey's and finally to Hermione's, who was once again crying quietly.
"What? Sorry Professor, but I don't understand.
"Harry, My boy. Are you in an – intimate – relationship?" Madame Pomfrey phrased her enquiry carefully.
"No" Still Harry was no clearer on their point, and Hermione now refused to look at him.
"What Madame Pomfrey means Harry is that you're..." Hermione cut Professor Dumbledore off.
"You're Pregnant Harry. With Child. Do you understand now?" She shouted at the boy-who-lived. Harry stilled.
"What? But I can't be. I'm Male" Harry was lost. Madame Pomfrey and Dumbledore stood still watching him.
"Do you understand now what you should have told me? If you'd told me you were GAY Harry I wouldn't have had a problem with it, but to hide it, to pretend…"
"But I'm not Gay."
"Harry, you must be. Male pregnancies only occur through Homosexual intercourse, there is no other reason for this pregnancy." Harry was shocked.
"Are-are you sure I'm pregnant." He whispered, his mind casting back briefly to the summer.
"Quite sure Harry." Dumbledore assured him, placing a hand on Hermione's shoulder.
"I'm not gay." Harry stated firmly. "I may have an explanation. But I'm not Gay." He lifted Hermione's hand from her lap. "I promise you Hermione, I do love you, I really do. And I am categorically not gay." She looked up at him, tears still leaking from her red-rimmed eyes.
"Then how Harry. Explain it to us."
"I- don't know if I can." He looked away from all of them for the first time, letting his eyes fall on a far corner of the room, finding immense interest in the pattern on the wallpaper.
He felt a hand under his chin, it gently pulled his face back round to the three waiting faces.
"Please, Harry. We want to understand." Hermione's pleading voice pulled at his heartstrings.
"Ok, I'll try." He told them shakily, "but it's hard."
"It was this summer…" He started, his mind flashing back to that wet summer night….
He'd been doing chores for the Dursley's all day long. It had started early that morning. He'd awoken to his alarm clock trilling out the hour, and had gotten out of bed quickly, going downstairs to make everybody else's breakfast, managing to sneak himself a piece of toast before Petunia came downstairs to oversee his work.
After breakfast, he'd been set to work on the household, being told that the whole house was due its spring clean. He's started on that and had almost finished, when, at four o'clock Vernon had shouted at him that he was to move onto working the garden now. The day was cool and grey, with a tendency to drizzle, and Harry shivered a little as he worked in just a t-shirt. He mowed both lawns, front and back, and trimmed the borders neatly. A man from down the road had walked past with his dog, and told Harry that he was definitely earning his pocket money today. Harry had just nodded to the man, and laughed grimly to himself when the man was out of sight. He continued with his work, weeding all the borders.
Finally it went dark and Harry was allowed inside for tea. His tiny plate of salad was hardly worth calling a meal, but he wolfed it down gratefully, before being shooed upstairs to bed.
It was then that things went downhill.
The Dursley's all followed him to bed, and the house quickly went quiet. He was sure that everyone was asleep and was about to get up and do some Homework, when he heard his bedroom door creak quietly open.
He jolted around to face the door, but could only see a silhouette in the doorway.
"Hello?" He tentatively whispered, unsure who was there. The figure stepped further into the room and closed the door behind him. As the figure approached Harry got a clear view of who it was. A familiar face. He relaxed instantly.
He climbed out of bed only for the large man to stand right before him, so close that their chests were touching. He could see the other man's face now, and it was contorted, a strange look on the familiar face.
"What's Going on?" He asked, worried now, about what was happening. The larger man gripped both of Harry's arms roughly, and pushed him backwards, so the back of his legs hit the edge of the bed with a thud.
"What? What's wrong …" Before he could speak the name his mouth was covered by the other mans. He pulled his head away.
"No. What are you doing?" He wondered vaguely why no one had heard his voice when it usually only took a creaky floorboard to waken Vernon.
The other man pushed Harry's shoulders down and back so the boy was forced down onto the bed. Harry realized with a shock what was happening all to clearly. He froze, knowing he couldn't fight the larger man off him.
He could feel hands grabbing at his waistband, pulling his trousers and boxers away.
"No. Please. Don't" He repeated over and over, unable to completely believe this was happening.
His lower half was now completely free of clothing, the other man seemed happy about this, and Harry lay in front of him shivering with the thought of what was to come.
Without warning, the large man grabbed at Harry's hips once more, and lifted the off the bed. He positioned himself between Harry's thin legs. Harry started struggling in earnest once more.
"Stop that" The dark figure growled. The voice sounding almost inhuman. Harry stilled once more. The large figure thrust heavily into Harry and he screamed with pain. White hot agony ran through him like electricity. Tears of humiliation coursed down his cheeks as the other man thrust repeatedly into him, over and over. Harry saw his eyes glaze over finally, and with a groan Harry felt warm liquid coat his insides, pouring salty pain into him. The man put two large hands on Harry's chest, holding him down as he pulled himself out. He stood, and turned around, walking out of the room without another word.
Harry lay there on his bed, warm liquid pooling on the bed beneath him, and sobbed.
He looked up at Hermione, Dumbledore and Madame Pomfrey, all of whom had anguish and shock written all over their faces. What ever explanation they had expected, this wasn't it.
"Oh Harry" Hermione whispered, and threw her arms around him as they both cried together.
Dumbledore put a hand on Madame Pomfrey's shoulders, and gently pulled her out of the hospital wing to talk, giving the two friends a little privacy.
Harry sat, with Hermione's arms around him and sobbed. He hadn't told them who it was, hadn't told them all how he'd felt, lying on his bed, in humiliation, as he watched the retreating back of his Godfather, Sirius Black.


text:
A driving school thought it would be a great idea to prank their new driving instructors, so they approached MaxMan.tv and pro driver Leona Chin to give the unsuspecting teachers the ride of their lives on their first day at work. As you'll see after the jump, the result was anger, futile attempts at instruction and sheer terror. We're sure that those who stayed on the job after this will now be ready for anything.
(via LS)


text:
Sunday, February 12, 2012
Swap-a Roo!
I am excited to be back! I have been swamped with work and life, but I feel like I am not whole without my blog. I am jumping back in with a swap. What better way to get back into the bloggy spirit than to send and recieve a box full of surprises in the mail!
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