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Great Escapes (Two of Them)
Fortune Smiles: Media Superstar Gloria Leonard takes us on a touchingly funny walk through the minds of two people who have been dating and are about the take the leap into bed. This touches on the insecurities one feels when making love for the first time, including the awkwardness of bringing up the use of a condom. Watch the sparks fly with the debut return to films by award winning actors. Winner of Adult Video News awards for Hottest Sex Scene and Best Actress. Starring: Richard Pacheco & Nina Hartley
The Tunnel: Beautiful surreal imagery highlights this mini-drama, directed by Candida Royalle. A young (18+) woman's recurring dream leads her to an encounter with a mysterious man who helps her discover her long hidden and explosive passion. Starring: Sibohan Hunter and Steve Lockwood.
Performers: Nina Hartley, Richard Pacheco, Sibohan Hunter, Steve Lockwood
Feature Runtime: 75 Minutes


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Atlanta Streetcar project spurs new development
History is repeating itself in one downtown Atlanta area with the revival of the streetcar, a project currently under construction that promises to be the next great thing in "transportainment" and bring development to a once struggling area.
The Atlanta Streetcar tracks stretch from Centennial Olympic Park to the Martin Luther King Jr. National Historical Site and the streetcar will provide a 2.7 mile loop of service. The east-west route, representing the first phase of the project, will be operational in early summer.
"This project will carry this historic district into the future," said Gene Kansas of Gene Kansas Commercial Real Estate, a developer in the area and the company charged with handling the marketing and leasing for the Sweet Auburn Curb Market. "This will be a catalyst for new development and is a great example of new urbanism."
Funded in large part by a federal grant, the streetcar project is encouraging business owners to give the area a second look, Kansas said.
"I bought the Atlanta Daily World Building at 145 Auburn Ave. about two months ago. Within 30 days of owning the property, I had a signed lease with Arden's Garden," Kansas said. "Folks like me are coming to that area because there is good opportunity and promise. I personally like the area because it is part of our historic fabric — not just in our community, but in our country. This is the birthplace of the Civil Rights Movement and the area is genuine and rich with history."
Part of that history encompasses the Sweet Auburn Curb Market, which, at 90 years old, is the oldest public market in the city.
"I think that the streetcar and market have a symbolic relationship," Kansas said. "The streetcar has 12 stops — one being the curb market and when I asked Tim [Borchers] why one was in front of the curb market he said that is because that is where people want to be and that is where riders can be found. So the market is a huge deal for the streetcar and the streetcar is a huge deal for the market."
City of Atlanta Streetcar Executive Director Tim Borchers said the increased mobility and convenience spurred by the Atlanta Streetcar is anticipated to generate investment opportunities on the more than 80 acres of underused land and in the 30 vacant buildings within a few blocks of the route. This translates to improved quality of life for residents, employees, students, visitors and shoppers in the area.
Projections, which Borchers calls conservative, predict a $2.56 return on investment for every $1 spent on the streetcar development. Portland's streetcar project, developed in 2001, gives him hope that Atlanta's project will be successful.
"In the first year, their streetcar carried 1.4 million people and by 2010 it carried 3.9 million annually," he said, adding more than $3.5 billion in private investment has been put in place within three blocks of the line. "That has certainly proven that as a transit system it is successful. The streetcar is like a steroid for local culture. Look at cities like Memphis or New Orleans — both rely on and are fed by a streetcar system."
And both cities are similar to Atlanta in that they have great entertainment, culture and history, he said.
"In Atlanta, we will see the culture and flavors of the area be developed further with more restaurants, bars and local music-themed entertainment venues and hotels to service those areas," he added.
Plus, the streetcar will be a convenient way for Atlanta's 21,000 unique conventioneers each week to get around, grab a bite to eat or see the sights via a safe, inexpensive transit option.
"I have seen how streetcars have helped communities all over the world and I have personally lived on, dated, socialized and worked successfully all on a streetcar system," he said.
Gwen Denninghoff, who co-owns Le Metro Creperie, a business that opened about two months ago at the curb market, said the streetcar project was a factor in locating there.
"We are originally from Zürich and Zürich runs on public transportation," Denninghoff said. "You can get anywhere without having a car so we have seen firsthand how well public transportation can work, and we wanted to be somewhere where people have access to that."
The curb market also offered the right feel, space and price to help them make a go at their business, which offers gluten-free crepes with a variety of fillings from savory to sweet.
"I think there is a subdued excitement right now in the area," she added. "There are a lot of different groups coming together to work on changing the area for the better. It is very similar to the work done to transform the Grant Park area. People there had a vision for what they wanted Grant Park to be and they worked together to make that happen. I think that is happening along Edgewood."
Matt Ruppert, owner of Noni's, a local family-owned Italian restaurant on Edgewood Avenue, opened his restaurant in 2008, before the streetcar project was conceived, because he has been a longtime supporter of downtown. Now the permanency of the streetcar lends itself to development, he said.
"These tracks aren't going anywhere so this route will never change. Bus routes change," he said. "I think when there is something like this that is institutional, it will add to the feeling of bustle and will help change people's perceptions of this neighborhood very quickly."
The permanency of rail tracks has been shown to spur economic development in other cities:
In Portland, the streetcar catalyzed 140 real estate projects worth $3.5 billion; following its construction, property values increased by approximately 50 percent.
In Seattle, a $51 million streetcar project (capital cost) led to 3.3 million square feet of development.


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Synaptic RuminationsIf You're Going to Use a Cane, Use a Cane!
Feb 27th, 2016 Cancer Survivors Needed for Quick Nutritional Survey
Feb 9th, 2016 "For the Rest of Your Life, Babe"
Jan 25th, 2016 "Catch That Look" Book Excerpt: Oh, Crap!
Nov 10th, 2015 "No More Secs!" Book Excerpt: Where's My Marcus Welby Moment?
Nov 9th, 2015 #VotingDay #VotingMatters
Nov 3rd, 2015 #NoBraDay Is Just Not Working For Me
Oct 13th, 2015 Celebrate International Literacy Day (Infographic)
Sep 8th, 2015 The Mask
Sep 1st, 2015 "No pictures, please"
Jul 8th, 2015 Feeling Good About "No More Secs!"
Jul 7th, 2015 It's not about giving in to MS, it's about taking charge of MS
May 26th, 2015 #SignOfSupport #4MSF
May 18th, 2015 Now you can find me at Healthline News...
Apr 9th, 2015 We have MS...but we are so much more than MS
Mar 26th, 2015
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Foundation Barriers Benefits Results Core Tracey's Tree Tracey's Story Collaborative working in action "The action of working with someone to produce something." Oxford Dictionary definition Team Goals Together Lack of Funding Ineffective Communication Hierarchy Poor co-ordination Blurring of roles Concerns over confidentiality A patient centred service NHS Plan (2000) Professional rivalries To conclude.... References Barlett, G., Sellman, D., Thomas, J. (2005). Interprofessional Working in Health and Social Care – Professional Perspectives. Basingstoke: Palgrave Macmillan.
Breastfeeding Network (2003) The Breastfeeding Network Guidance Document. Retrieved from - http://breastfeedingnetwork.org.uk/pdfs/Guidance%20Document.pdf
CAADA. (2009). The CAADA-DASH MARAC Risk Identification Checklist. Retrieved from http://www.caada.org.uk/marac/RIC_FAQs.pdf
CAIPE. (2007). Creating an Interprofessional Workforce: An Education and Training Framework for Health and Social Care in England. Retrieved from http://www.caipe.org.uk/silo/files/cipw-fw-doc.pdf
Children's Workforce Development Council. (2009). The Common Assessment Framework for Children and Young People A Guide for Practitioners. Retrieved from https://www.education.gov.uk/publications/eOrderingDownload/CAF-Practitioner-Guide.pdf
CMACE. (2011). Saving Mothers Lives – Executive Summary. Retrieved from http://www.centreformidwiferyeducation.ie/news/cmace-2011-saving-mothers-lives
Coram Children's Legal Centre. (2012). Child Protection Project. Retrieved from http://www.protectingchildren.org.uk/cp-system/
Department for Children, School and Families. (2007). The Children's Plan. Retrieved from https://www.education.gov.uk/publications/eOrderingDownload/Childrens_Plan_Summary.pdf
Department for Children, School and Families. (2010). Working Together to Safeguard Children. Retrieved from https://www.education.gov.uk/publications/eOrderingDownload/00305-2010DOM-EN-v3.pdf
Department of Health. (2000). Framework for the Assessment of Children in Need and their Families. Retrieved from http://www.dh.gov.uk/prod_consum_dh/groups/dh_digitalassets/@dh/@en/documents/digitalasset/dh_4014430.pdf
Department of Health. (2000). The NHS Plan: A plan for investment, a plan for reform. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_4002960
Department of Health. (2001). Working Together, Learning Together. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_4009558
Department of Health. (2003). Every Child Matters. Retrieved from http://image.guardian.co.uk/sys-files/Society/documents/2003/09/08/EveryChildMatters.pdf
Department of Health. (2004). National Service Framework For Children, Young People and Maternity Services. Retrieved from http://www.dh.gov.uk/prod_consum_dh/groups/dh_digitalassets/@dh/@en/documents/digitalasset/dh_4090552.pdf
Department of Health. (2007). Creating an Interprofessional Workforce – an education and training framework for health and social care in England. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_078592
Department of Health. (2007). Maternity Matters: Choice, access and continuity of care in a safe service. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_073312
Department of Health. (2008). A High Quality Workforce – NHS Next Stage. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_085840
Department of Health. (2009). Healthy Child Programme. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_107563
Department of Health. (2010). Equity and Excellence – Liberating the NHS. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_117353
Department of Health. (2010). Midwifery 2020: Delivering Expectations. Retrieved from http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_119261
General Social Care Council. (2002). Code of Practice for Social Care Workers. Retrieved from http://www.gov.im/lib/docs/socialcare/services/Codes_of_Practice.PDF
Hammick, M., Freeth, D., Copperman, J., Goodman, D. (2009). Being Interprofessional. Cambridge: Policy Press.
Health and Care Professions Council. (2012). Standards of Proficiency Social Workers in England. Retrieved from http://www.hpc-uk.org/assets/documents/10003B08Standardsofproficiency-SocialworkersinEngland.pdf
Health and Social Care Act (2012). Chapter 7. Retrieved from http://www.legislation.gov.uk/ukpga/2012/7/pdfs/ukpga_20120007_en.pdf
International Confederation of Midwives. (2011). ICM International Definition of a Midwife. Retrieved from http://www.internationalmidwives.org/Portals/5/2011/Definition%20of%20the%20Midwife%20-%202011.pdf
Kings Fund. (2008). Safe Birth, Everybody's Business. Retrieved from http://www.kingsfund.org.uk/publications/safe-births-everybodys-business
Loxley, A. (1997) Collaboration in Health and Welfare – Working with difference London: Jessica Kingsley Publishers Ltd.
Mickan, S. (2005). Evaluating the effectiveness of health care teams. Australian Health Review, 29(2), 211-217.
National Health Service. (2009). Guidance for Safeguarding Children and Young People in General Practice. Retrieved from http://www.sglos-pct.nhs.uk/clinical/GP%20guidance%20final%20doc.pdf
National Institute for Health and Clinical Excellence. (2006). Routine postnatal care for women and their babies. Retrieved from http://www.nice.org.uk/CG037
National Institute for Health and Clinical Excellence (2006) Promotion of breastfeeding initiation and duration. Retrieved from http://www.nice.org.uk/niceMedia/pdf/EAB_Breastfeeding_final_version.pdf
National Institute for Health and Clinical Excellence. (2008). Antenatal care: routine care for the healthy pregnant woman. Retrieved from http://nice.org.uk/nicemedia/live/11947/40145/40145.pdNICE (2007) Antenatal and postnatal mental health
National Institute for Clinical Excellence. (2010). Pregnancy and Complex Social Factors. Retrieved from http://www.nice.org.uk/nicemedia/live/13167/50822/50822.pdf
NHS Act (2006). Chapter 41. Retrieved from http://www.legislation.gov.uk/ukpga/2006/41/contents
NSPCC. (2012). Supporting Professionals to Take Action Against Neglect. Retrieved from http://www.nspcc.org.uk/Inform/resourcesforprofessionals/neglect/policy-briefing_wdf92079.pdf
Nursing and Midwifery Council. (2004). Midwives Rules and Standards. Retrieved from http://www.nmc.uk.org/Documents/Standards/nmcMidwivesRulesandStandards.pdf
Nursing and Midwifery Council. (2007). Medicine Management. Retrieved from http://www.nmc-uk.org/Documents/Standards/nmcStandardsForMedicinesManagementBooklet.pdf
Nursing and Midwifery Council. (2008). The Code: Standards of conduct, performance and ethics for nurses and midwives. Retrieved from http://www.nmc-uk.org/nurses-and-midwives/the-code/the-code-in-full/
Nursing and Midwifery Council. (2010). Record Keeping. Retrieved from http://www.nmc-uk.org/Documents/Guidance/nmcGuidanceRecordKeepingGuidanceforNursesandMidwives.pdf
Royal College of Midwives. (2006). Domestic Abuse: Pregnancy, Birth and the Puerperium. Retrieved from http://www.rcm.org.uk/college/policy-practice/guidelines/rcm-position-statements/position-statements/
Royal College of Midwives. (2012). Early Breastfeeding. Retrieved from http://www.rcm.org.uk/college/policy-practice/guidelines/practice-guidelines/
The Children's Act (2004). Chapter 31. Retrieved from http://www.legislation.gov.uk/ukpga/2004/31/pdfs/ukpga_20040031_en.pdf
The Childcare Act (2006). Chapter 21. Retrieved from http://www.legislation.gov.uk/ukpga/2006/21/pdfs/ukpga_20060021_en.pdf
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Data from: Large fluctuations in the effective population size of the malaria mosquito Anopheles gambiae s.s. during vector control cycle
Date Published: July 9, 2013
DOI: http://dx.doi.org/10.5061/dryad.35rs4
Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data.
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|The excel sheet labeled Data contains the genotypes of 791 Anopheles gambiae females for 13 microsatellite loci analyzed in the manuscript: Hodges et al. 2013. Large fluctuations in the effective population size of the malaria mosquito Anopheles gambiae s.s. during vector control cycle. Evolutionary Applications (in press). The format is that of typical microsatellite genotype data, which can be easily imported into the GENALEX program and also formatted into input files for a number of genetic data software programs. The genotype data is arranged as 1 population for each time point with the most recent time point first: Pop1 is April 2010, Pop2 is November 2009, Pop3 is September 2009, Pop4 Is July 2009, Pop5 is May 2009, and Pop6 is March 2009.
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LITTLE ROCK (AP) Dallas Cowboys owner Jerry Jones says drafting a wide receiver isn't a priority this year, even after the departure of Terrell Owens.
"One of the reasons that I made the decision on Terrell Owens was because I thought so much of our young receivers. Not only Roy Williams ... but our other young receivers — Miles Austin, Sam Hurd, those are guys that will now have a chance," Jones said Thursday night. "It would really have to be a real opportunity for us to go there.
"The good news with our team right now is we can go about any direction we want to in the draft and pick the best player."
Owens was released last week and later signed with the Buffalo Bills.
Jones, a former Arkansas Razorbacks player, was in Little Rock to be honored by Easter Seals as its 2009 Arkansan of the Year. He was accompanied by three Cowboys: quarterback Tony Romo, tight end Jason Witten and running back Felix Jones.
There were reports of a rift last season between Romo, Witten and Owens. Reporters were not permitted to talk to Romo or Witten on Thursday night.
Jerry Jones did speak briefly to reporters about next season.
"We've got a great core base put together. We've made some changes in the offseason. We think that's going to really be very helpful — both sides of the ball and on special teams," he said. "We've got a chance to get as many as nine players in the draft, and we'll need about 80% of those to be players."
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text:
Differential transcript profiling through cDNA-AFLP showed complexity of rutin biosynthesis and accumulation in seeds of a nutraceutical food crop (
Plant genomics
Buckwheat, consisting of two cultivated species
Differential transcript profiling through cDNA-AFLP in seed maturing stages (inflorescence to seed maturation) with 32 primer combinations generated total of 509 transcript fragments (TDFs). 167 TDFs were then eluted, cloned and sequenced from
The present study concludes that in addition to structural genes, other classes of genes such as regulators, modifiers and transporters are also important in biosynthesis and accumulation of flavonoid content in plants. cDNA-AFLP technology was successfully utilized to capture genes that are contributing to differences in rutin content in seed maturing stages of
In tartary buckwheat, fagopyritols; mono-, di- and trigalactosyl derivatives of D-chiro-inositol account for 40% of total soluble carbohydrates compared to 21% in common buckwheat thus, helps in the treatment of diabetes
Molecular basis of nutritional superiority, particularly higher rutin content in
Figure 1
Flowers to seed maturation stages of
Flowers to seed maturation stages of
Figure 2
Rutin content variation: rutin content variation during seed developmental stages of
Rutin content variation: rutin content variation during seed developmental stages of
Flavonoid content in a particular tissue and developmental stage is largely influenced by different classes of regulatory genes, transporters, modifiers
Identification and analysis of differentially expressed transcripts (TDFs)
cDNA-AFLP analysis on RNA samples from flower to mature seed stages of rice-tartary and common buckwheat with 32 primer pair combinations resulted in the identification of 42 clear and unambiguous fragments (TDFs). The TDFs ranged in sizes from 150–750 bp representing a total of 2,584 TDFs.
A total of 167 differential TDFs based on presence/absence or differences in intensity were eluted from the gels, re-amplified and sequenced; Figure
Additional file 1
Table S1. Sequence homology: Functional characterization of transcript derived fragments (TDFs) based on BLAST X and TAIR FASTA analysis.
Click here for file
Figure 3
cDNA-AFLP patterns: Rice-tartary (R) vs Common Buckwheat (E) in seed maturation stages (6, 7, 8, and 9)
cDNA-AFLP patterns: Rice-tartary (R) vs Common Buckwheat (E) in seed maturation stages (6, 7, 8, and 9).
Figure 4
Functional classification of transcript derived fragments: Transcript derived fragments categorized into different categories
Functional classification of transcript derived fragments: Transcript derived fragments categorized into different categories.
TDFs representing genes encoding alanine glyoxlate, methionine sulfoxide, fatty acid desaturase, KAS III, sucrose 6 phosphatase, ubiquitin protein ligases etc. were implicated in the biosynthesis of proteins & amino acids (12%), fatty acids (7%) and carbohydrates (6%). Similarly, 8% of TDFs corresponded to genes for key enzymes involved in secondary metabolism, including the flavonoid and anthocyanin biosynthesis. TDFs (10%) involved in cellular function included genes coding for pectin acyltransferases, proline rich extensins, glycine rich proteins, and arabinogalactan etc. TDFs corresponding to transporters (13%) included ABC transporters, auxin hydrogen symporter, sugar transporters, zinc and potassium transporters. Genes involved in signal transduction (14%) and regulation (18%), including Zn finger binding proteins, Leucine rich repeats calmodulin binding protein, protein kinases and transcription factors belongs to MYB and WRKY classes were also detected.
TDFs representing differentially expressed genes were classified into different categories on the basis of their presence/absence (qualitative variation) or differences in amount of expression (quantitative variation) between both the
Figure 5
Functional classification of differentially expressed TDFs in
Functional classification of differentially expressed TDFs in
TDFs representing genes involved in transport of metabolites (ABC and sugar transporters, auxin hydrogen symporter), regulation of biosynthesis (MYB TF, Zn finger protein), metabolism of metabolites (ubiquitin protein ligases, extensin protein), signal transduction (calmodulin binding protein) and energy transfer (ATP CF0 subunit) were chosen for quantitative RT-PCR analysis in different seed maturing stages of both the
Transcript quantification of selected TDFs in flower to seed maturation stages of
Four TDFs representing genes for Ubiquitin protein ligase (JN982742), ABC transporter (JN982732), sugar transporter (JN982735) and MYB118 (JN982734) showed significantly increased transcript abundance in flowers (S6) of rice-tartary compared to common buckwheat with 2.86, 4.71, 7.36 and 11.42 folds expression, respectively; Figure
Figure 6
Expression of TDFs in different tissues of
Expression of TDFs in different tissues of
Understanding types and number of genes differentially expressed during seed maturation would help in discerning molecular mechanisms contributing to nutritional superiority, including high rutin content in the seeds of
Large numbers of TDFs with differential expression pattern were observed in different seed developmental stages of both the
The flavonoid content increase in buckwheat seedlings has been attributed to the increase in the concentration of sucrose
Significantly higher expression of 4 TDFs representing genes for ubiquitin protein ligase, ABC transporter, sugar transporter and calmodulin binding protein in S7 of rice-tartary buckwheat compared to
Transcription factors are known to play important role in various seed development processes and regulation of secondary metabolism
Ub protein ligases (JN982742) are known to regulate various biological processes like photomorphogenesis, hormonal response, senescence, floral, embryo and seedling development through degradation of proteins as well as regulates phenylpropanoid pathway during UV stress and sugar signaling during seedling development
The present study concludes that in addition to structural genes, the other classes of genes such as regulators, modifiers and transporters are equally important in contributing to higher flavonoids content and nutritional superiority of
Plant material
Seeds of
Isolation of genomic DNA, RNA and cDNA synthesis
Genomic DNA was isolated from leaf samples of both the
First strand cDNA was synthesized from 5 μg of RNA-free from DNA (RNA preparation was treated with 2U of DNAse I), reverse transcribed by using M-MuLV reverse transcriptase (GeNeiTM) and an oligo-dT (12–18) primer. Double-stranded cDNA was synthesized according to a standard double-stranded cDNA synthesis protocol
cDNA-AFLP analysis
About 500 ng of double stranded cDNA was subjected to standard AFLP template production according to Vos et al.
Additional file 2
Table S2. Primers List: Primers used for cDNA-AFLP analysis.
Click here for file
Transcript-derived fragment (TDF) isolation and re-amplification
Differentially expressed TDFs based on presence, absence or differences in intensity were cut with a sharp blade from the gel with care to avoid any contaminating fragment(s), eluted in 50 μl of sterile double distilled water, incubated at 95°C for 15 min and then hydrated overnight at 4°C. An aliquot of 2 μl was used for re-amplification in a total volume of 25 μl, using the same set of corresponding selective primers and PCR conditions as used for the selective amplification, except that an annealing temperature of 56°C for 35 cycles was used. PCR products were resolved in a 2% agarose gel; each single band was isolated and eluted using the Genaxy DNA gel extraction kit (Genaxy Biosciences Inc., USA). The reproducibility of cDNA AFLP was verified by repeating the experiment twice.
Cloning and sequencing of TDFs
Eluted TDFs were cloned into the plasmid pGEM-Teasy® vector (Promega Corp., Madison, WI) following the manufacturer's protocol and then sequenced. Sequences of TDF (with vector sequences trimmed off, where plasmid was used as the template) were then analyzed for their homology against the publicly available non-redundant genes/ESTs/Transcripts in the database (
Real-time RT-PCR analysis
Specific primer pairs were designed for TDFs; Additional file
Additional file 3
Table S3. Primers List: Primers used for real time qRT-PCR analysis.
Click here for file
Competing interests
The authors declare no competing interests.
Authors' contributions
NG carried out the experiments, analyzed the data and drafted the manuscript. PKN participated in data analysis. RSC proposed and supervised the research, helped to draft and reviewing the manuscript. All authors read and approved the final manuscript.
The authors are thankful to the Department of Biotechnology, Ministry of Science & Technology, Govt. of India for providing research grant to RSC.
Evidence for protective and antioxidant properties of rutin, a natural flavones, against ethanol induced gastric lesions
Selected dietary flavonoids are associated with markers of inflammation and endothelial dysfunction in U.S. women
Genetic and genome resources in Buckwheat- Present and Future Perspectives
Fagopyrum wenchuanense and Fagopyrum qiangcai, two new species of Polygonaceae from Sichuan, China
Advances in the development of functional foods from buckwheat
Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercitrin
Fagopyritols, D-chiro-inositol, and other soluble carbohydrates in buckwheat seed milling fractions
Composition and technological properties of the flour and bran from common and tartary buckwheat
Tartary buckwheat breeding (Fagopyrum tataricum L. Gaertn.) through hybridization with its Rice-Tartary type
De novo sequencing and characterization of floral transcriptome in two species of buckwheat (Fagopyrum)
Differential expression of flavonoid biosynthesis genes and accumulation of phenolic compounds in common buckwheat (Fagopyrum esculentum)
Differential Expression of Anthocyanin Biosynthetic Genes and Anthocyanin Accumulation in Tartary Buckwheat Cultivars 'Hokkai T8' and 'Hokkai T10'
Expression of flavonoid biosynthesis genes vis-à-vis rutin content variation in different growth stages of Fagopyrum species
Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics
Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway
Buckwheat Fagopyritols. Advances in buckwheat research
Fagopyritol B1, O-a-D-galactopyranosyl-(1 → 2)-D-chiro-inositol, a galactosyl cyclitol in maturing buckwheat seeds associated with desiccation tolerance
Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development
Methods for transcriptional profiling in plants. Be fruitful and replicate
MATE2 mediates vacuolar sequestration of flavonoid glycosides and glycoside malonates in Medicago truncatula
Studies on nutritional aspects of tartary buckwheat
Influence of sucrose on rutin content and flavonoid biosynthetic gene expression in seedlings of common buckwheat (Fagopyrum esculentum Moench)
Sugars as signal molecules in plant seed development
Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis
Increasing sucrose concentrations promote phenylpropanoid biosynthesis in grapevine cell cultures
Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis
Flavonoids Are Differentially Taken Up and Transported Long Distances in Arabidopsis
Calmodulin and calmodulin-binding proteins in plants
Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants
Sugar sensing and Ca2 + −calmodulin requirement in Vitis vinifera cells producing anthocyanins
A review of extensins in plant cell wall
Developmental localization andthe role of hydroxyproline rich glycoproteins during somatic embryogenesis ofbanana (Musa spp. AAA)
Too many transcription factors: positive and negative interactions
Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression
Transcription factors regulating the progression of monocot and dicot seed development
Identification and disruption of an Arabidopsis zinc finger gene controlling seed germination
A RING-H2 zinc-finger protein gene RIE1 is essential for seed development in Arabidopsis
Transcription factors controlling plant secondary metabolism: what regulates the regulators?
Ubiquitin-conjugating enzyme (E2) confers rice UV protection through phenylalanine ammonia lyase gene promoter unit
Analysis of the differential expression of the genes related to Brassica napus seed development
Rapid isolation of high molecular weight plant DNA
Molecular Cloning: A Laboratory Manual (3rd ed.).
AFLP: a new technique for DNA fingerprinting
The Arabidopsis Information Resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and community


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Bulgarian Property Exchange
I have always thought this was a brilliant idea and in today's economic climate, where it is not easy to sell a property here in Bulgaria and you often have to take a loss, the idea of exchanging your property for another is a good solution.
There are a number of sites that offer international swaps (one country for another), however, this page will just be to allow people to list properties they own in Bulgaria and are interested to swap the property for another in a different part of the country. Maybe you bought a ski apartment and would not like to try the coast, or vice versa. Or maybe you have been living close to a holiday resort and have decided you would like to swap for the peace and quiet of a rural inland village. Or maybe you would like to consolidate various properties you have and swap for one larger or higher value property. Alternatively you may just want to move to a different area of the country.
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Civil War History article//Status of the list
Expand Messages
- I haven't seen it...how about telling us about it?
While I'm here, I may as well point out that the list currently has 37 sub-
scribers. Over half seem to be outside Alabama!
Don't be bashful, folks...feel free to speak up!
AJ Wright//alabamahistory moderator
- Dear Moderator,
Can't say I'm surprised that your subscribers are substantially from
outside the state. Those of us who are interested in Alabama topics but
geographically distant feel sort of isolated; we lack the sort of face
to face discussion of the issues that locals take for granted.
As for the Paul Horton article, it is in the latest issue of Civil War
History--which, for those of you who haven't seen it, is the most
scholarly of the journals dealing with this topic of intense popular
interest. I haven't got the article at hand, but it is a case study
of Lawrence County during the war.
The argument (from memory) is a familiar one by this point, that there
was substantial reluctance to secede in this north Alabama county, and
widespread disaffection from the Confederacy by the middle phases of
the war. My sense is that the research for the piece is unusually
good, that Horton has turned up a great deal of material seldom
encountered even by specialists in the field. In one striking
instance, Horton finds evidence that the main Confederate official in
the area, General Roddy, solicited reluctant draftees with the promise that he
would keep them close to home--and that he was engaged in indirect
negotiations with the Union command for a separate peace well before
the end of the war.
None of this, I suspect, is too startling, but it is the best detail
study I've seen in Alabama. The most novel thing I saw is the
author's assertion that the anti-Confederates were not that based
among poorer non-slaveholders--the Winston County image of Unionism--
but that anti-secession and anti-war sentiment were cross-class
alliances, with plenty of Tennessee Valley planter participation.
I have my doubts, but it is an interesting position, and other
scholars are coming up with similar conclusions.
Thanks, Michael Fitzgerald
> From: AJ Wright <MEDS002@...>
> I haven't seen it...how about telling us about it?
> While I'm here, I may as well point out that the list currently has 37 sub-
> scribers. Over half seem to be outside Alabama!
> Don't be bashful, folks...feel free to speak up!
> AJ Wright//alabamahistory moderator
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GONZALES — Gonzales City Councilman Gary Lacombe resigned Tuesday, almost two weeks before a recall election targeting him and fellow Councilman Timothy Vessel.
Lacombe, who was two years into a tumultuous first term of office, delivered his resignation letter by hand to the Louisiana Secretary of State's Office on Tuesday, canceling his planned recall election Dec. 6, officials said.
Lacombe said in the letter that he was resigning for "personal reasons" and touted his "major accomplishments" while also lamenting unnamed people for spreading "negativity" about the council.
Among those accomplishments, he wrote, were a balanced city budget and the council's "record of rejecting proposed zoning changes that would have given unfair advantages to certain property owners over others."
Lacombe said he had also donated one-half of his pay as a councilman to fund 13 merit scholarships, to date, to East Ascension High School graduating seniors.
"Despite these and other significant accomplishments, certain individuals have continually spread negativity and misinformation to our citizens about the efforts of the City Council to follow its ordinances and to position Gonzales for an even brighter future," Lacombe wrote.
Budget cutting and the rezoning votes, however, helped fuel the recall effort. Critics and recall supporters claimed that those and other council decisions were the hallmark of a voting bloc by Lacombe, Vessel and Terance Irvin on the five-man council and have been undoing the city's well-being.
A group of residents called Save Gonzales launched the recall effort against Lacombe and Vessel in April.
The group did not try to recall Irvin, who has been on the council since 2000.
Gonzales Mayor Barney Arceneaux, who has clashed with Lacombe, said he learned late Tuesday afternoon about Lacombe's resignation.
"The city has been through a rough period of time these last couple of years. We obviously feel this is a necessary step toward resolving our issues and moving the city forward," Arceneaux said in a statement.
"I think this is a good step," he said of Lacombe's resignation. "I certainly hope the residents of the city will take the final step and vote for the recall of Councilman Vessel."
Chuck LeBlanc, chairman of the recall effort, echoed the mayor's comments, saying, "Save Gonzales thinks it was the right thing for him (Lacombe) to do."
Lacombe's departure along with an interim replacement who will be appointed by the City Council has the potential to break the three-man voting bloc on the council.
Vessel, who still faces a recall election Dec. 6, said Tuesday evening that he was not aware of Lacombe's resignation and declined further comment.
"This is my first I'm hearing about it," Vessel said.
Irvin did not return a message left on his cellphone Tuesday.
In a letter to Gonzales City Clerk Clay Stafford advising the city of Lacombe's resignation, Erin Delaney, an administrator with the Louisiana Secretary of State's Office, said the city has until Dec. 15 to appoint someone to fill Lacombe's seat until a special election can be held.
The council must ask for a special election so voters can elect someone to fill the remainder of Lacombe's term, which ends Dec. 31, 2016.
The next scheduled elections are the March 28 primary election and the May 2 general election, with qualifying dates of Feb. 4-6.
Meg Casper, spokeswoman for the Secretary of State, said Lacombe's name will remain on the ballot Dec. 6 because ballots have already been printed.
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Neither Lacombe nor his attorney, Charles Spencer, returned calls for comment Tuesday evening.
A letter from Lacombe's attorney and Lacombe's letter of resignation also were filed with the Ascension Parish Clerk of Court about 3:44 p.m. Tuesday.


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Sitting right on the beach of the southern Turkish coast, the Saturn Palace Resort & Hotel enjoys a peaceful location east of Antalya city. If you're looking to enjoy a beach break, the hotel is ideally situated, on the beautiful Lara Beach, one of Turkey's popular coastal resorts. It is here that you can enjoy some time spent on the hotel's private section of the soft golden sands, in the crystal clear waters of the sea, or trying some of the water sports on offer.
Both adults and children will enjoy a stay at the Saturn Palace Resort & Hotel, with enough activities and entertainment on offer to please every member of the family. A kid's club is available for little ones, giving parents the chance to enjoy some days spent alone. A children's playground and a lively outdoor wave swimming pool are also great features for little ones.
Couples can also enjoy a romantic break at the hotel and some time alone in the sun, at the quieter pool, which offers a more peaceful vibe.
There is a range of places to eat and drink at the hotel, which include a buffet restaurant that serves meals throughout the day and well into the evening. You can also dine at the Ottoman barbecue, sample some sea food at the fish restaurant and enjoy some Italian specialties at the Italian restaurant. Drinks are served from the poolside bars, snack bar, lobby bar and Vitamin Bar.
Distance from Airport:
16 km from Antalya Airport
The Saturn Palace Resort & Hotel's rooms are modern and stylishly designed and you can choose between standard and VIP Jacuzzi rooms to Family and Grand Family rooms. Every room comes with air conditioning, satellite TV, a mini bar, safe, telephone and private bathroom and balcony.
Transfer time:
25 minutes
Location and Resort:
The Saturn Palace Resort & Hotel is found along the southern coast of Turkey, in the lovely beachside resort of Lara Beach. It is around 17 kilometres from the centre of Antalya city.
Eating and Drinking:
You can enjoy a range of cuisine at the hotel's restaurants, from Italian and seafood to barbecue and traditional Turkish dishes. Bars can be found by the pool, in the lobby and in the spa.
Swimming pools:
The hotel has a total of three swimming pools, one of which is found inside, in the spa. Outside, you will find a children's pool, water slides and a more relaxing pool, all of which are surrounded with sun loungers and parasols.
Right choice:
The hotel manages to please both couples and families, with a range of different settings, as well as numerous activities and entertainment for both adults and children.
Suitable for:
Couples and families.


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Kanaputs is an interpreter for Java. It runs Java code without compilation, as each instruction is executed when you write it. Kanaputs can be embedded very easily into an XML document to add programmatic features. For example, Kanaputs is fully integrated with Ant and provides a way to execute Java within an XML Ant build file. The grammar of the Kanaputs language is very close to the Java grammar but adds some flexible features such as typeless variables and reactive variables.
DDNS is P2P software that aims to build a decentralized, distributed DNS. The domain name service (DNS) is a worldwide distributed database that still has centralized elements. This project elaborates the feasibility of a decentralized, distributed DNS (DDNS). For that purpose, a peer-to-peer distributed hash table is used that is similar to the Kademlia protocol.
Surftree is an applet that displays data organized in a spreadsheet as an expandable tree on a Web page. The tree can be 9 levels deep and have over 65,000 nodes. Each node can display up to 4 independent Web pages or JPG images. It works with Java 1.0 and 1.1+ and in any Web server environment.
sfick (simple file integrity checker) is a system that assists system administrators with the process of verifying the integrity of critical files. Given a system compromise, system administrators must be able to verify the integrity of any files they designate as critical and, if such files have been modified in any way, have the ability to restore an original copy of such a file. It stores backup copies of files designated as critical along with verification information to write-once media such as a CD-R.


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European Parliament CULT Committee update
During its last meeting, the CULT committee was very active in the field of education and training. It adopted the "Erasmus+ and other tools to foster mobility in VET" draft report and a draft opinion on the "2014 Discharge for the EU general budget". It also discussed the draft report on the "Follow-up of the Strategic framework for European cooperation in education and training (ET2020)" presented by Rapporteur MEP Krasnodębski (ECR). A highlight of the meeting was the exchange of views with Commissioner Tibor Navracsics in which the priorities for education, training, culture, youth and sports in 2016 were discussed. In the field of education and training the debate mostly tackled the skills agenda that according to the Commissioner should go far beyond employability and VET, the Paris Declaration and concrete initiatives for its implementation and a set of measures for the integration of refugees (including supporting validation systems and online language courses).


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As the exclusive three Antique Shell Dikes in the world, the only one in China is in Huanghua city of Cangzhou, whose forming and abundant ancient ocean cultural relics has drawn much attention from scholars home and abroad. In September of 1988 the provincial natural preservation of Antique Shell Dike in Huanghua city was founded.
It is said that Antique Shell Dike in Zhaojiabao of Nanpaihe Town in Huanghua City consists of living things like shells,
There are 6 Antique Shell Dikes in Huanghua city, distributed nearly paralleled with modern coastal lines, with a longitude of about 31square kilometers, which took shape respectively from about 6000 or 7000 years ago to about 1000 years ago. According to the evidence from the scientists, these antique shell dikes are natural sea shield formed by weathering myriads of shells and its scraps carried by waves when tides rise and fall in the Bo Sea in the long term.
Being the most intact one in the world so far, the Antique Shell Dike in Huanghua city has been an important outcome in the process of eastern coast of Bo Sea's taking into land shape since 7000 years ago, whose developmental scale, lasting time and the contained geographical information of ancient environment are rare throughout the world and playing an important role in the 4th international geographical research. It can provide natural script for the research of ancient ocean transformation and environment change trend, of important scientific value for predicting future environment change trend. Moreover, its formation and abundant ancient ocean cultural relics have drawn attention of scholars home and abroad since the 1920s.
The 5 antique shell dikes on the land are buried deeply underground except the 2nd one naked on the surface. The 2nd antique shell dike, used to be a precious natural sea shield with a total length of 60 kilometers or so stretching from Tianjin in the north to Haixing in the south, is about 2 to 3 meters high with a breadth of about 1500 meters from the east to west, covered by wild jujube trees and rich in many rare medicinal materials such as Shaseng, Chinese ephedrine, jujube seeds, Tuyuan, etc. Because of luxuriant plants, it is also a paradise for the fox, badger and wild rabbits. The soil on the antique shell dike is soft, good for storing rainwater so that if a hole is dug on antique shell dike the sweet water will leak endlessly. The antique shell dike not only prevents the tide for the village but also is the natural navigation aids for the fishermen, when they see the green dike in a far distance on the sea just like they see their cozy home.
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Bar Harbor
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About Bar Harbor
Mt. Desert Island's beautiful rugged coastline and dramatic interior was the inspiration for 19th Century artists Thomas Cole and Frederic Church, whose paintings then inspired people to visit. And by the turn of the century, Bar Harbor was one of America's most dreamed about summer resorts. Today the best places to stay in Bar Harbor are still those with the best artist's view. And cutely, our Guru's list of the best places to stay in Bar Harbor seems to include only wonderfully traditional wooden structures painted in shades of blue.
Now on the cruise ship route, Bar Harbor can be quite busy, and the downtown area has plenty of souvenir stores, cafes and ice cream parlours. But it's not so busy to have lost its charm, and its visitor friendliness makes it very easy to book sailing trips, try sea kayaking, do some whale watching, schedule in some rock-climbing, or just enjoy yourself in the evenings in the bars and restaurants.
Just outside town is the Acadia National Park, with its wonderful landscapes, both strange - like Bubble Rock, and ordered - like the Wild Gardens of Acadia. The geological diversity of this place is astounding. Here you'll find thick woodland, sheer granite cliffs and remote, placid lakes in lush valleys.
Our pick of the best places to stay in Bar Harbor is ideally placed to enjoy everything from the great outdoors to the shops, cafes and restaurants.
- Painted blue and white, Shore Path Cottage is a lovely family run B&B with ten rooms - some with beautiful ocean views. This is a great place for hikers, bikers and those who want to get out on the ocean as the owners will help you make the most of Bar Harbor's great outdoors.
Still not sure which is the best place to stay in Bar Harbor for you? Browse through our list of all the best places to stay in Bar Harbor, expand your search to include the whole of Maine, or contact one of our friendly Gurus for advice.
Best Hotels in Bar Harbor
Shore Path Cottage 10 rooms from $145Bar Harbor
A lovely family run B&B in Bar Harbor with ocean views and a large garden. Rooms are large & traditional & there's a homely atmosphere and warm welome. The family will give you all the advice you could possibly want about ocean activities, and hiking & biking in the local national park.Views - The garden and some of the rooms have lovely views of Frenchman's Bay and the spectacular Porcupine IslandsSights nearby - Close to Acadia National ParkGreat walks - Good for walkers.Back to Nature


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Lil Dicky Tour dates 2017
Lil Dicky tickets are on sale right now on ConcertPass. ConcertPass is your number one destination for all Lil Dicky concerts tickets as well as concert dates and extensive tour information. On ConcertPass you'll find Lil Dicky tickets at great prices across an excellent range of seating options. We'll let you pick your seats from our interactive maps so you can find the seats that are right for you. All Lil Dicky concerts tickets come with our ticket integrity guarantee, with all tickets guaranteed or your money back. You'll find tickets for thousands of concerts and events on ConcertPass, so please feel free to browse around. Be a part of the action and witness great some performances right now, buy your tickets for Lil Dicky today, so you don't miss out.
Sep 01 2017 3:30 AM
North Coast Music Festival - 3 Day PassUnion Park
Sep 03 2017 1:00 PM
Electric Zoo Festival - Sunday Admssion
Sep 22 2017 3:30 AM
Life is Beautiful Festival - 3 Day Pass
Sep 23 2017 3:00 PM
Life is Beautiful Festival - Saturday Admission
Oct 07 2017 1:00 PM
Rare Orlando 2017: Travis Scott, Carnage, Lil Dicky, ZHU, Kyle & Graves
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Lili Hudson, EFT Expert, Hypnotist, & Personal Cheerlleader Reminds Us That Our MInds Are Not Books Easily Read
Understanding your needs, wants, and desires is very important; communicating those desires and needs is critical to effective communication and ultimate internal and external peace and ease.
About Lili Hudson: Known for getting results, Lili Hudson, EFT Expert, Hypnotist, and Personal Cheerleader offers a refreshing solution to today's problems. With a background in science and a passion for serving, Lili Hudson searched for solutions to tackle the challenges of weight, sadness, anxiousness, frustration, and anger. During her search, Lili discovered the success experienced when EFT (Emotional Freedom Technique) and self-hypnosis are combined. Drawing on her experience, Lili has developed several transformative programs including "Be A Better Student" and the breakthrough "Slender, Strong and Fit." In this signature program, Lili helps clients unhook from food issues, eating less food with more enjoyment, dropping the pounds and the struggle without pills, injections or scary, risky surgery. By understanding the problem clearly, Lili shows clients how to resolve it and dissolve it quickly. Lili reminds clients: Life Happens…Tap. A volunteer practitioner with The Vets Stress Project, Lili is a resident of the Nashville area. An avid hiker, Lili has been married for 28 years and has 2 adult children. She considers herself a lifelong student, fascinated with understanding how people tick, and then helping people tick better! She and her husband are passionate boaters and enjoy turning off the static of life every chance they get. Lili can be reached through www.LiliHudson.com, and emailed at firstname.lastname@example.com
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Unlot, or Hugh, was a Ruddy Orion man who lived in the 23rd century.
Unlot was tahedri of his family. His family was an old one, dating back at least 5 centuries. Their first villa had stood for nearly 300 years before being destroyed by fire, and was replaced by another that stood for over 200 years. It had many additions, though Unlot considered even the 200-year-old sections to be too 'modern', and missed the earlier building.
He also represented a company that made (possibly defective) nose-filters, and guaranteed they wouldn't send someone blind.
He hosted E. D. Fitzgerald (telling him to call him by the Human name "Hugh"), and treated him to lunch and a tour of the family estate. Later, they attended a traditional Orion banquet with members of the Gamashes family and Flugol Corporation. Fitzgerald later wrote about his time with Unlot in Spacing to Byzantium: Travels in the Orion Colonies. (FASA RPG module: The Orions: Book of Common Knowledge)
- It is not clear if Unlot is a member of the Gamashes family or if he represents or even runs Flugol Corporation.


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A decent defensive play and one for the yield investors is how one leading City broker described GlaxoSmithKline plc (LON:GSK) ahead of third-quarter figures next week. JP Morgan Cazenove also said it expects the international drugs giant to restate its guidance for the year of 11-12% growth in earnings per share (EPS), although it admits "this could ultimately prove conservative". For the three months ended September, the broker said revenue growth measured in the local currencies would grow by 4% from £6.13bn this time last year. EPS is set to increase by 10% from 23p. "We see a solid quarter, and we also expect consensus to make minor upgrades on a reported basis post the quarter, reflecting recent foreign exchange moves, though the recent forex moves are likely already partly reflected in the current share price," JPMC said in a note to clients. The challenges for GSK are those posed by Brexit, which may actually provide a short-term foreign exchange rate boost, and life after Advair. The asthma drug is GSK's biggest selling product by far – racking up revenues of £3.8bn last year – but it has come off patent. This means it is subject to cheap competition. Analysts worry what will follow Advair as the GSK pipeline appears bereft of blockbusters to take its place. JPMC reckons the stock is fairly valued at just over 15 times EPS but sees GSK as a "fairly safe defensive play in the near-term, particularly appealing to yield investors". The dividend yield is a very decent 4.7%. Of the 17 analysts polled by the Broker Forecasts website, seven are sitting on the fence alongside JPMC. There are eight 'buyers' and only two 'sells'. Up over a fifth in the year to date, shares in GSK marked time in early afternoon trade at £16.62. That values the business at £81bn, making it Britain's fifth largest quoted business.
Original Article: A solid, defensive play for yield investors, but just what could sink GSK?NEXT ARTICLE
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Top 10 biotech and pharmaceutical companies worldwide based on market value in 2015 2015 ranking of the global top 10 biotech and pharmaceutical companies based on revenue (in billion U.S. dollars) Johnson & Johnson, U.S. 74...
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text: The 3-star Meridiana Hotel is located close to the historically important Piazza della Liberta and can be easily reached by guests arriving either by train or by car; the Palazzo dei Congressi is no more than 10 minutes` walk away. With the majority of renovations completed in 2002, the hotel now offers modern rooms equipped with air conditioning, satellite TV, hair dryers and direct dial telephones. A continental style buffet breakfast is available to all guests although some may wish to enjoy the first meal of the day in the comfort of their own rooms. A moderately priced hotel, the Meridiana welcomes business and leisure travellers alike; assuring both of a comfortable and enjoyable stay.