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Neural expression and post-transcriptional dosage compensation of the steroid metabolic enzyme 17β-HSD type 4
Steroids affect many tissues, including the brain. In the zebra finch, the estrogenic steroid estradiol (E2) is especially effective at promoting growth of the neural circuit specialized for song. In this species, only the males sing and they have a much larger and more interconnected song circuit than females. Thus, it was surprising that the gene for 17β-hydroxysteroid dehydrogenase type 4 (HSD17B4), an enzyme that converts E2 to a less potent estrogen, had been mapped to the Z sex chromosome. As a consequence, it was likely that HSD17B4 was differentially expressed in males (ZZ) and females (ZW) because dosage compensation of Z chromosome genes is incomplete in birds. If a higher abundance of HSD17B4 mRNA in males than females was translated into functional enzyme in the brain, then contrary to expectation, males could produce less E2 in their brains than females.
Here, we used molecular and biochemical techniques to confirm the HSD17B4 Z chromosome location in the zebra finch and to determine that HSD17B4 mRNA and activity were detectable in the early developing and adult brain. As expected, HSD17B4 mRNA expression levels were higher in males compared to females. This provides further evidence of the incomplete Z chromosome inactivation mechanisms in birds. We detected HSD17B4 mRNA in regions that suggested a role for this enzyme in the early organization and adult function of song nuclei. We did not, however, detect significant sex differences in HSD17B4 activity levels in the adult brain.
Our results demonstrate that the HSD17B4 gene is expressed and active in the zebra finch brain as an E2 metabolizing enzyme, but that dosage compensation of this Z-linked gene may occur via post-transcriptional mechanisms.
Steroids have profound effects on the brain. In the zebra finch (
The gonads do not supply the steroids that direct masculinization of the song circuit, but the brain may be an essential source of E2 both during development and in adulthood
Evidence exists to support both theories. Many of the major steroid -producing and -transducing factors that can act on the song circuit are present in the zebra finch brain
Indeed, in mammals and in zebra finches, data is accumulating that male brain cells are fundamentally different than female cells by virtue of their different complement of sex chromosomes: XX/XY in mammalian females and males, and ZW/ZZ in avian females and males, respectively. There is evidence that genes on the sex chromosomes might influence sexual differentiation of the zebra finch song system
Here, we examine 17β-hydroxysteroid dehydrogenase type 4 (HSD17B4), a steroidogenic enzyme that converts E2 to a less potent estrogen, estrone (E1). Inasmuch as HSD17B4 is present and active in particular brain regions, it could regulate the local concentrations of E2. This could impact the organization and function of the song circuit
We therefore used several techniques to examine HSD17B4 DNA, mRNA, and enzyme activity in male and female zebra finch brains. We used posthatch day 5 (P5) and adult birds because at both ages, E2 meaningfully impacts the song system. P5 is within the period when steroid administration has the greatest masculinizing effect on song nuclei, other steroid-synthesizing genes are expressed in the brain at this early age, and it is just prior to the earliest emergence of identifiable song control nuclei
Zebra finch HSD17B4 gene and cDNA sequences
We used two independently cloned cDNAs as probes for the molecular experiments. The clone used for Bacterial Artificial Clone (BAC) library screening, and Northern and Southern blot hybridizations was an Expressed Sequence Tag (EST; GenBank Accession No.
Figure 1
Zebra finch HSD17B4 gene and cDNA clone identities
Zebra finch HSD17B4 gene and cDNA clone identities. (A) HSD17B4 gene model and EST structure based on the zebra finch genome assembly. The 5' end of the gene is to the left; a scale of the Z chromosome positions is on the bottom in 0.01 Mb increments. The gene model shows the coding exon structure. The zebra finch EST
Prior to the SoNG EST project, we had cloned a HSD17B4 cDNA that we used as the riboprobe template for our brain
To further confirm that the molecular tools we used were indeed HSD17B4, we examined the cDNA clone sequences in relation to the gene sequence in the zebra finch genome assembly. Using the Apollo Genome Annotation Curation Tool, we manually annotated the zebra finch DNA sequence from the genome assembly, the nucleotide sequences of the two clones we used for experiments, and other available transcript sequences to confirm the HSD17B4 gene model
Confirmation of Z chromosome localization
We examined the intensity of hybridization on a Southern blot that contained male and female genomic DNA and to test if we could detect a sex difference in HSD17B4 gene abundance. To control for the possibility that gene products derived from one enzyme digestion could provide spurious results, we digested the genomic DNA with two enzymes, HaeII and HindIII, which results in two different banding patterns (Figure
Figure 2
Males have more Z chromosome-linked HSD17B4 DNA
Males have more Z chromosome-linked HSD17B4 DNA. (A) Southern blot hybridization of HaeIII and HindIII digested genomic DNA from male (M) and female (F) of zebra finch probed with 32P-labeled zebra finch HSD17B4 EST
To confirm the Z chromosome localization of HSD17B4 gene, we used the EST to first screen a zebra finch BAC library
Northern blot hybridization shows sex difference in mRNA levels
Northern blot hybridization for male and female whole brain or gonads showed higher expression of HSD17B4 gene in males relative to females (Figure
Figure 3
Northern blot hybridization shows sex difference in HSD17B4 mRNA levels
Northern blot hybridization shows sex difference in HSD17B4 mRNA levels. A Northern blot of brain and gonad total RNA from male (M) and female (F) zebra finches was hybridized with a 32P-labeled
Sex differences and song system localization of HSD17B4 mRNA
Figure 4
At P5, we detected HSD17B4 hybridization throughout the telencephalon and in the area that resembled the lateral magnocellular nucleus of the nidopallium (LMAN) in male and female brains. This was the only brain area that resembled a song nucleus that was visible at this young age, based on its neuroanatomical position and boundaries (Figure
We also detected HSD17B4 mRNA in the adult male HVC (used as a proper name) nucleus of the song circuit, though we were unable to perform a quantitative sex comparison because HVC was not reliably identified in comparable sections in females (Figure
HSD17B4 activity does not show a sex difference
Several initial experiments were performed to first optimize the conditions for the final biochemical assessments of HSD17B4 activity in the brain. A timecourse experiment showed that a 5 minute incubation was optimal for accurately measuring HSD17B4 activity (Figure
Figure 5
HSD17B4 biochemical assay optimization
HSD17B4 biochemical assay optimization. (A) Results from timecourse experiment for biochemical meaures of HSD17B4 activity. Graph shows fmol E1/μg protein produced after brains were incubated for 10 minutes, 30 minutes or 90 minutes with and without cold trap. (B) Results from dose response curve for biochemical meaures of HSD17B4 activity. Graph shows fmol E1/μg protein produced after incubation of brain with 10 nM, 50 nM, 100 nM, 300 nM of 3H-E2 substrate for 5 minutes. Each point represents the average of duplicate measurements for each substrate concentration. 50 nM 3H-E2 substrate is centered in the linear rise of enzyme activity.
Using the optimized conditions, we first analyzed HSD17B4 activity in the telencephalon and diencephalon of adult male and female birds. Two-way ANOVA for sex, region and the sex by region interaction showed that there was no significant overall effect of sex (p = 0.745), brain region (p = 0.206), or the interaction (p = 0.263) on HSD17B4 activity levels (Figure
Figure 6
Results of biochemical assays for HSD17B4 activity in male and female brain regions from two experiments
Results of biochemical assays for HSD17B4 activity in male and female brain regions from two experiments. (A) Comparison of HSD17B4 activity in whole telencephalon and diencephalon shows no significant differences between sex, brain region, or the interaction of sex by brain region. There is a subtle trend in the male towards higher activity in the telencephalon compared to male diencephalon and both female regions. (B) Comparison of HSD17B4 activity in two sub-regions of the telencephalon and the diencephalon showed no overall sex differences but a significant sex by brain area interaction (p = 0.017). (C) HSD17B4 activity levels measured in testes and ovaries are significantly higher than those in the brain (p < 0.001) but do not show a significant sex difference (p = 0.062). Error bars are ± s.e.m.
In a separate set of birds, we performed another assay for HSD17B4 activity in two telencephalic regions that contained brain regions that
Estradiol is a potent masculinizing steroid for the developmental growth of the sexually dimorphic song circuit in the zebra finch. Estradiol also continues to impact the function of song regions in adult birds
We confirmed the Z chromosome localization of the HSD17B4 gene, and that the abundance of HSD17B4 DNA and brain mRNA is approximately two-times greater in males a than in females, consistent with previous reports
The higher levels of HSD17B4 expression in the male brain compared to the female brain, in particular within song control nuclei, were counterintuitive given extensive evidence that E2 masculinizes these areas
We therefore assayed the activity levels of HSD17B4 in adult brains. Our results demonstrate that this enzyme is active in metabolizing E2 in the zebra finch. However, in two major brain regions and in two experiments, the conversion of E2 to E1 was not significantly different between adult males and females - the sex difference in HSD17B4 mRNA levels in the adult brain does not translate into a sex difference in activity levels. Therefore, while the neuroanatomical mapping of HSD17B4 mRNA suggested that this enzyme may influence the sex-specific organization and function of the song control circuit by reducing the availability of E2 in males, our biochemical results indicate that post-transcriptional mechanisms may regulate neural HSD17B4 activity to minimize the sex difference found in HSD17B4 expression levels.
Perhaps regulatory mechanisms that are known to modify enzyme activity, such as phosphorylation and availability of substrate or cofactors, could explain our results. These factors might override the tendency for greater enzyme activity based on the male bias in mRNA abundance. Enzymes in particular have been theorized to be relatively dosage insensitive because their function is often more strongly dependent on properties other than the copy number of the genes encoding them
HSD17B4 is a Z-linked enzyme that shows a sex difference in DNA dose and mRNA expression levels, but not in activity levels. The post-transcriptional regulation of HSD17B4 may signal that E2 metabolism is tightly controlled in the brains of both male and female zebra finches.
All zebra finches were obtained from colonies housed in the UCLA Life Science Vivarium. The Chancellor's Animal Research Committee approved all protocols.
HSD17B4 gene and cDNA clone annotation
Two zebra finch HSD17B4 cDNA clones were independently produced and used for probe templates in subsequent experiments. We cloned one cDNA from zebra finch brain using PCR and plasmid cloning techniques as previously described
At the time both clones were produced, their identities as HSD17B4 sequences were based on homology matches to genes in other species such as human and chicken. To do this, we aligned the predicted amino acid sequence of the EST to the predicted amino acid sequence of the human, chicken, zebra fish and mouse HSD17B4 genes to assess the extent that the zebra finch sequence shares sequence and functional domain motifs with known HSD17B4 genes from other species.
Recently, the zebra finch genome has been sequenced and assembled
Southern blot hybridization
Zebra finch genomic DNA for Southern blot analysis was isolated from heparinized blood. Briefly, the nuclei of blood cells were suspended in 10 mM Tris-HCl (pH 8.0), 100 mM EDTA (pH 8.0) and incubated in the presence of 0.5% sodium dodecyl sulfate (SDS), 100 μg/ml proteinase K at 50°C overnight. The mixture was extracted successively with phenol saturated with TE [10 mM Tris-HCl (pH 8.0), 1 mM EDTA] and chloroform. The supernatant was ethanol precipitated, washed with 70% ethanol and resolved in TE. The purified genomic DNA was stored at 4°C until use.
Male or female genomic DNA was digested HindIII or HaeIII, then electrophoresed in a 1% agarose gel, 1× TAE (4 μg/lane). The gel was denatured with 0.5 M NaOH-1.5 M NaCl for 30 minutes, rinsed in water and neutralized in 1 M Tris (pH 7.6)-1.5 M NaCl for 30 minutes. After another wash in 20× SSC for 20 minutes, the gel was transferred to MAGNA Nylon Transfer membrane (Osmonics Inc, Minnetonka, MN) in 20× SSC overnight. The membrane was washed in 2× SSC, dried and baked at 80°C, then UV cross linked. The DNA for probes was agarose gel purified from restriction enzyme digested plasmids using the Gene Clean kit (Q-BIOgene, Solon, OH). Hybridization with a 32P-labeled DNA probe was carried out in a hybridization buffer [0.5 mol/L Na-phosphate buffer (pH 7.2), 7% SDS, 1 mmol/L EDTA, 1% BSA (A3059, Sigma, St. Louis, MO)] at 65°C overnight. The membrane was washed in 2× SSC, 0.1% SDS at room temperature for 1 minute twice and 65°C for 30 minutes twice, and then subjected to autoradiography.
Hybridization was performed with an expressed sequence tag (EST) clone from the ESTIMA database (GenBank Accession No.
The HSD17B4 zebra finch bacterial artificial chromosome (BAC) clone, 094L04, was isolated from a zebra finch BAC library made by the Arizona Genomics Institute
Northern blot hybridization
RNAs for male and female adult zebra finch brain or gonads were isolated using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer's instructions, and stored at -80°C until use. RNA samples (8 μg/lane) were subjected to denaturing agarose gel electrophoresis and capillary transferred to a MAGNA Nylon Transfer membrane (Osmonics Inc) in 10× SSC. The membrane was hybridized with a 32P-labeled probe based on
The density of the bands for brain and gonad samples was quantified in Image J
P5 and adult brains were collected, flash frozen, and stored at -80°C until use. Delicate P5 brains were retained in the skull to prevent damage from dissection. Both male and female brains from each age (n = 4 per sex for P5, n = 6 per sex for adults) were sectioned to 20 μm for
After hybridization, slides were washed and dehydrated as previously described
To quantify optical density of select brain regions, digital pictures of film exposures were first converted to grayscale in Adobe Photoshop, then the average density of each region was measured in Scion Image
Image production
HSD17B4 activity
To measure HSD17B4 activity in brain tissues, we used the core biochemical procedure previously described for other steroidogenic enzymes
Since the activity levels of HSD17B4 enzyme have not previously been studied in the zebra finch, we first performed several experiments to optimize conditions for its measurement. To determine the most accurate period of tissue incubation, we performed a timecourse experiment. We used two adult female whole brains, and after homogenization, samples were incubated with 100 nM 3H-E2 and incubated at 41°C for 10, 30, or 90 minutes. Results were plotted to determine a time of linear activity levels. To determine if the addition of an E1 "cold trap," radioinert E1, would enhance the detection of 3H-E1 by limiting the conversion of 3H-E1 to other steroid products, 1 μg E1 was added to half of the tubes in a preliminary experiment. Lastly, to determine the optimal 3H-E2 concentration to use for substrate, a female whole brain was incubated in the presence of 10, 50, 100, or 300 nM 3H-E2 and processed as above.
Utilizing the conditions determined by preliminary experiments (5 minute incubation with 100 nM 3H-E2 and no cold trap), we then performed two experiments to measure the HSD17B4 activity in dissected brain areas. First, we assayed HSD17B4 levels in the telencephalon and diencephalon of adult males and females (n = 5 per sex). Second, we further dissected the telencephalon into two parts: the dorsomedial telencephalon ("DT," tissue between the midline and 2 mm lateral to midline starting 5 mm from the rostral tip of the brain and terminating 5 mm from the caudal end of the telencephalon, a region that contains the hippocampus and lateral ventricles), and the caudodorsal telencephalon ("CT," a 1.5 mm deep section containing the caudal half of the dorsal telencephalon that contains HVC). We also assayed the diencephalon, ovaries and testes from each bird (n = 5 per sex, except CT n = 4 per sex due to technical issues). One- and two-way ANOVA and Tukey post-hoc tests (SPSS) were used to test for effects of sex and brain region on HSD17B4 activity.
Authors' contributions
SEL designed experiments, interpreted data, wrote the manuscript and created figures; YI designed, conducted, and interpreted experiments, wrote the manuscript and created figures; VAL, PW, PSE, and RKO conducted experiments; APA, and BAS designed experiments, interpreted data, and wrote the manuscript. All authors read and approved the manuscript.
We thank Kathy Kampf for excellent technical assistance. All authors have read and approved the manuscript. This project was supported by NIH R01 MH061994 (to BAS).
Sexual dimorphism in vocal control areas of the songbird brain
Aromatase is pre-synaptic and sexually dimorphic in the adult zebra finch brain
Forebrain steroid levels fluctuate rapidly during social interactions
Elevated aromatase activity in forebrain synaptic terminals during song
Subcellular compartmentalization of aromatase is sexually dimorphic in the adult zebra finch brain
Cloning of the zebra finch androgen synthetic enzyme CYP17: a study of its neural expression throughout posthatch development
Widespread capacity for steroid synthesis in the avian brain and song system
Estrogen synthesis in the male brain triggers development of the avian song control pathway in vitro
Local intracerebral implants of estrogen masculinize some aspects of the zebra finch song system
Developmental regulation of the distribution of aromatase- and estrogen-receptor-mRNA-expressing cells in the zebra finch brain
Steroidogenic enzymes along the ventricular proliferative zone in the developing songbird brain
Neurosteroid production in the songbird brain: A re-evaluation of core principles
Birdsong and the neural production of steroids
Distribution and regulation of telencephalic aromatase expression in the zebra finch revealed with a specific antibody
Brain is the major site of estrogen synthesis in a male songbird
Circulating estrogens in a male songbird originate in the brain
Neurosteroids and brain sexual differentiation
Isolation and characterization of a zebra finch aromatase cDNA: In situ hybridization reveals high aromatase expression in brain
Activities of 3beta-HSD and aromatase in slices of developing and adult zebra finch brain
Effects of embryonic treatment with fadrozole on phenotype of gonads, syrinx, and neural song system in zebra finches
Neither testicular androgens nor embryonic aromatase activity alters morphology of the neural song system in zebra finches
Functional testicular tissue does not masculinize development of the zebra finch song system
Sexual differentiation of the zebra finch song system parallels genetic, not gonadal, sex
Sexual differentiation of the zebra finch song system
Neural, not gonadal, origin of brain sex differences in a gynandromorphic finch
A putative 5 alpha-reductase inhibitor demasculinizes portions of the zebra finch song system
Antiandrogen blocks estrogen-induced masculinization of the song system in female zebra finches
Antiestrogens fail to prevent the masculine ontogeny of the zebra finch song system
Sex chromosomes and brain gender
Sexually dimorphic expression of trkB, a Z-linked gene, in early posthatch zebra finch brain
Activities of aromatase and 3beta-hydroxysteroid dehydrogenase/delta4-delta5 isomerase in whole organ cultures of tissues from developing zebra finches
Zebra finch estrogen receptor cDNA: cloning and mRNA expression
Expression of androgen receptor mRNA in zebra finch song system: developmental regulation by estrogen
Expression of estrogen receptor and aromatase mRNAs in embryonic and posthatch zebra finch brain
Expression of androgen receptor mRNA in the late embryonic and early posthatch zebra finch brain
5 beta-reductase and other androgen-metabolizing enzymes in primary cultures of developing zebra finch telencephalon
Aromatase and 5 beta-reductase activity in cultures of developing zebra finch brain: an investigation of sex and regional differences
Sexual differentiation of brain and behavior in the zebra finch: Critical periods for effects of early estrogen treatment
Subcellular compartmentalization of aromatase is sexually dimorphic in the adult zebra finch brain
Early estrogen treatment alone causes female zebra finches to produce learned, male-like vocalizations
Early estrogen treatment of female zebra finches masculinizes the brain pathway for learned vocalizations
Post-hatching inhibition of aromatase activity does not alter sexual differentiation of the zebra finch song system
Effects of long-term flutamide treatment during development in zebra finches
Paradoxical hypermasculinization of the zebra finch song system by an antiestrogen
Androgen effects on the development of the zebra finch song system
Neither testicular androgens nor embryonic aromatase activity alters morphology of the neural song system in zebra finches
Sex differences in structure and expression of the sex chromosome genes CHD1Z and CHD1W in zebra finches
A Birds-Eye View of Sex Chromosome Dosage Compensation
Enhanced gene expression in the forebrain of hatchling and juvenile male zebra finches
Sexual differentiation of the zebra finch song system: Potential roles for sex chromosome genes
Faced with inequality: chicken do not have a general dosage compensation of sex-linked genes
Comparison of the chicken and zebra finch Z chromosomes shows evolutionary rearrangements
Regional differences in dosage compensation on the chicken Z chromosome
Brain aromatase: new lessons from non-mammalian model systems
Estrogen synthesis in the brain--role in synaptic plasticity and memory
Estrogen-mediated structural and functional synaptic plasticity in the female rat hippocampus
Comparison of the chicken and zebra finch Z chromosomes shows evolutionary rearrangements
The sexually dimorphic expression of androgen receptors in the song nucleus hyperstriatalis ventrale pars caudale of the zebra finch develops independently of gonadal steroids
Expression of Androgen Receptor mRNA in Zebra Finch Song System: Developmental Regulation by Estrogen
The Songbird Neurogenomics (SoNG) Initiative: Community-based tools and strategies for study of brain gene function and evolution
Steroids, fatty acyl-CoA, and sterols are substrates of 80-kDa multifunctional protein
Molecular characterization of mouse 17 beta-hydroxysteroid dehydrogenase IV
Apollo: a sequence annotation editor
The genome of a songbird
Structure of the gene for the human 17beta-hydroxysteroid dehydrogenase type IV
Dosage compensation is less effective in birds than in mammals
Molecular cloning of zebra finch W chromosome repetitive sequences: Evolution of the avian W chromosome
Disruption of FEM1C-W gene in zebra finch: Evolutionary insights on avian ZW genes
Brain estrogens rapidly strengthen auditory encoding and guide song preference in a songbird
Birdsong "transcriptomics": Neurochemical specializations of the oscine song system
Chromosome-wide gene-specific targeting of the Drosophila dosage compensation complex
Global analysis of X-chromosome dosage compensation
Dosage compensation of the active X chromosome in mammals
The molecular basis of dominance
Chromosomal polymorphism and comparative painting analysis in the zebra finch
Activities of aromatase and 3β-hydroxysteroid dehydrogenase/Δ4-Δ5 isomerase in whole organ cultures of tissues from developing zebra finches
The expression of the sex steroid-synthesizing enzymes CYP11A1, 3beta-HSD, CYP17, and CYP19 in gonads and adrenals of adult and developing zebra finches
Androgen synthesis in a songbird: a study of cyp17 (17alpha-hydroxylase/C17,20-lyase) activity in the zebra finch


text: Simply Orchestra Look Inside Simply Orchestra Piano - Easy Piano28 Well Known Masterpieces. Arranged by Robert Schultz. Masterworks; Piano Collection; Piano Supplemental. Simply Series. Masterwork Arrangement; Recital. Book. 80 pages. Published by Alfred Music (AP.30047). Usually leaves our warehouse: Within 4 to 6 business days More info Add to Cart { "catalogRefId":"19247318", "productId":"prod19247318", "quantity":"1" } 1 Review

subset: commoncrawl


text:
I pray you'll be my eyes
And watch her where she goes
And help her to be wise
Help me to let go
Every mother's prayer
Every child knows
Lead her to a place
Guide her with your grace
To a place where she'll be safe
"If you would give me just a moment," Terrance excused himself slowly, uncertain. He still didn't trust the beautiful young woman before him. Her brackround checks came back oddly vague. Tracing her credit was nearly impossible. She knew all of this, he could see it in those deep green eyes, but she was playing it cool, acting innocent with that lovely and serene smile. He wanted to believe that beautiful face and that angelic expression, but instinct told him that she was hiding something. After years in the buisness, he'd learned to trust his instincts.
Catie watched the dark-suited buisnessman close the door behind himself, his eyes on her all the while. She kept her smile light, pleasant. He didn't trust her, and she knew it. She had hoped he would be less intelligent and easier to manipulate but, as usual, the universe was converging against her. However, she was just lucky enough that he was leaving her alone in the office. She had approximately five minutes, so she had to act quicklyly. The door latched slowly, but as the last click sounded, Catie released her breath, dropping her fake expression and running her hand through her thick, dark blonde hair. She surveyed the room quickly, clueless as to where to start. Without any other recourse, she jumped out of her chair and sprinted behind the desk, opening drawers and digging as fast as she could.
Anything, anything at all! Even if it's just a piece of paper, it's a piece of paper that could mean everything. She sifted through as fast as she could, the mumbling of the bankers on the other side of the wall was bleeding through, making her paranoid of every grunt and sudden outburst. Another drawer pushed closed as silently as she could; how could there be nothing there? Her informant assured her that the information was in that office. Catie dug and dug, but nothing even remotely close to what she was looking for.
She was running out of time. In a final effort, she grabbed the end of the desk in a firm grip with both hands. Using every muscle in her well toned arms, she lifted the mahogany desk and gave it one hard shake. A small thump responded, from under the desk. A manila folder was laying underneath, the papers inside worked out of their containment. As quietly as possible, Catie lowered the desk to the floor, and scrambled under the desk for the folder, flipping it open and speed reading it's contents. Jackpot! She began backing herself out on her hands and knees.
Suddenly, Catie stopped. Something had caught her eye. Between the carpet and the wall, something white and rectangular was visible. Catie reached out and tried to pull at it. It was not only tucked under the carpet, but it seemed to also be hidden behind the striped wallpaper. With a glance over the desk to be sure the coast was clear, she dipped back underneath, pulling a swiss army knife from her bra. She pulled it open and began cutting away the wallpaper around the rectangle. Just a small, exposed piece of the wall was left in it's place, she figured the man would never even miss it. It was a bit more of a struggle to pull it from under the carpet, but with a nice firm tug, the bit of paper came free from its binding with only a clean tear through the bottom of it.
Quickly, Catie stuffed the old, crumpled, folded, and half-wallpapered note into her new-found folder. She came out from under the desk in one swift movement, making her way around the desk and stuffing the manila folder into her briefcase. As soon as the briefcase clicked closed, the door clicked open and the dark-suited man entered.
"Ms. Wells, I'm very sorry. But, due to your lack of credit, I can't authorize any kind of loan at this time," his hands were linked in front of him, and only Catie would immediately notice that his knuckles were white.
"Well," Catie picked up her briefcase briskly, a solid, unshaking hand pushing her hair out of her face, "I was hoping that your bank would be more understanding than the others I've been to today, but I suppose I was wrong. Thank you for your time," she shook his hand... he was shaking. Her exit was swift and brisk, the faster she walked, the more the butterflies in her stomach eased.
"This was all I could find," Catie dropped the folder on the table in front of her collegue. "I stripped that office, this was all there was."
"This will be enough," Andy flipped through the folder slowly. "We'll send some of it up to the lawyers we're in contact with. You did a great job, Catie."
Catie slipped her hand into the deep pocket of her green sundress, assuring herself that the secret paper was safely against her person. "Thanks. I have to get back, Andy. I'd rather not be seen here if I can help it."
"I understand," he shook her hand, and then came around to open the door of the conference room for her. "Cate," he set a warm hand on her shoulder, "your grandfather will be really proud of you. This will keep you all safe for a long time. You should be really happy right now!"
"I am!" she attempted to assure, but her face couldn't support it. Andy gave her a knowing look and she smiled shyly at him. "I've got something on my mind. I'm fine. I just got some... really weird information."
"Anything useful?" he looked hopeful.
"Oh, no! Nothing like that. It's just... it's kind of personal. Don't worry," she gave him a quick hug. "Everything will be fine. Thanks for everything!" And with that, she dashed out of the building, quick as a flash.
The water below her flowed effortlessly, a soft babbling sound rising as it found pockets in the silt, and slapped against the rocks. Catie leaned over the stone and iron bridge on top of the little brook, the brown, crumpled and torn paper spread out in her hands. The sun was setting, casting an orange glow on the nearly translucent paper. She'd read it at least ten times now, peices of it were even memorized at this point. She just couldn't stop. The words gripped her like none other had, nor would they ever again in her life.
September 26th, 1989
To whomever may find this, my name is Catherine Chandler. I have been held prisoner for approximately 6months now. My time is coming to an end. This will be my last chance to attempt to communicate with the outside world. I know this, I can feel it. I can hear it in the voices of each of the men who escort me and threaten me. The only shred of compassion I have been shown has been from the doctor in charge of my health. He's given me this paper and a worn pencil that I can't do any harm with. Sometimes I think he worries that I'll kill myself. Sometimes I worry too. He is a good man in a bad situation, not unlike myself, and he knows the importance of putting one's thoughts on paper. He's tried to escape just as many times as I have, I'm sure.
I don't know who you are, or how long it's been since my death, but I would like to think that I still deserve a final wish. My captors would have me think of myself as an animal; unimportant, and expendable. But, I know I'm a human being, and I remind myself of it every day. I'm a 33 year old woman nearing the end of a process I have dreamt of for many years now. I am carrying a child.
It's a boy. A beautiful, healthy boy. I've seen him in my dreams, laughing and happy. He has his father's bright blue eyes, and my dark blonde hair. He looks like my dad when he smiles, and he'll smile even when he has every reason to be sad. He is perfect, and sometimes I cannot contain my sadness in the knowledge that I will never know him.
I don't know what will become of him once I am gone. I can only hope and pray that his father can find him. I can only hope and pray that I will find a way to tell his father of our child. I only hope and pray that he finds me before I'm gone. If I must leave this world without him, I hope and pray that his is the last face I see before I am taken from him.
Perhaps it is unfair of me to ask this of you, whoever you are, but I need to at least send this request out into the universe. Please, look after my son. He will be vulnerable for a long time before he grows strong enough to be the leader he is destined to be. He will be like his father one day, but until then he will need someone to protect him. He is different, despite any outward appearance, he will be someone the world has never known. No one like him has ever existed. It is important that you know this. He is special, in every way that you could possibly imagine. He is the reason I am being held here, kept alive until I can give him to the world.
I have been working on this letter for three days now. I don't think I'm even writing this for you anymore. I just have to say what's been in my heart for months. I need someone to know, even if it's only in my imagination, even if this letter is lost forever, even if you never exist. It is almost comical to me. I have spent three years now, hiding from you all. Shielding my life as best I can from strangers, and even my closest friends. Now, I beg your help. Is this some kind of horrible karma?
I think you have a right to know, my baby's father; his name is Vincent. Vincent! I say his name every day to remind myself that he was real. He IS real! I didn't dream him, he wasn't a hallucination, we have a child! And we loved each other, deeper and more pure than anything I have ever felt in my life. I ache for his touch every day, but knowing how it felt when he touched me (like a flash of lightening between us) keeps me sane. Our lives depended on each other, we always moved in such perfect sync. When I thought I was going to lose him, I couldn't imagine living without him and I fought for his life. Now, there is nothing he can do, and I weep for him after I am gone.
Please, if you are real, and you can locate my son, tell him how sorry I am. I lost my mother when I was young, but I at least have the memory of her. He will have nothing of me. He will have Vincent, I'm sure of that, but I will have to rely on you telling him that, for these months that he's been inside me, despite my situation, I have been so truely happy. He has made me so happy! Tell him that his grandmother had cancer, and his grandfather had a heart problem, so be sure to keep healthy. Tell him that, no matter what his father says, this was no accident. I truely believe that all of this has happened for a reason. His father and I found each other so that we could know what pure, unfiltered love was. We loved each other so that we could create him, so that he could be the wonderful person he will become. Because he will be such an amazing person, I was taken prisoner. And, as much heartache as it will cause for all of us, I died for a reason. I had to leave my lover and my son, never because I wanted to, but I have to believe, out of my death, something beautiful will come to them. His father's side of the family always seem to endure hardships before something beautiful falls into their laps. Tell him, it's the same with the Chandlers. Please, tell him to tell his father that I love him and I will wait for him to join me in the afterlife. But, tell him that I will be very angry with him if he doesn't join me with happy stories of our son's childhood, and his adult life, and our grandchildren, and our great-grandchildren. I want to know what the world looked like with our son in it, and I expect him to tell me. The road will be hard for him, for both of them, but they are strong and resiliant, and I hope that their thirst for life will be stronger than their grief.
I have run out of space for the thousands of other things I want to say. However, I find it hard to finish this letter. So final. As if it's conclussion will mark the end of me. I will miss the world. I will miss both of my worlds. Know that I will fight. I will not submit to death easily, and I pray with everything in me that this letter won't be necessary, but something deep inside tells me that I won't be here much longer. The sun is setting now, always my favorite time of day; as if, for a few minutes, the gap between day and night are bridged, and the two worlds touch each other. Whomever, wherever, you are, look at the next sunset for me. I'll be there beside you.
Catie swallowed the lump in her throat, she had already cried three times throughout the course of her many readings. She looked out over the little brook. Between the trees of Central Park, the sun was setting. Orange and pink light radiating from the sky as hardly an eighth of the sun could be seen on the horizon. A tear fell again as she shook her hair out of her face. It really was beautiful, the way the sun greeted the moon, and everything seemed warm and comfortable no matter the season. As the sun disappeared and dusk set in, Catie watched, thinking of Catherine Chandler, trying to reach out and feel her. Tears fell anew when she ackowledged the strangely comforting smell in the wind, and the tingling warmth filling her body. And then it was gone, and Catie folded the precious letter neatly, clutching it close to her heart.
"Catherine Chandler?" Ethan sat cross-legged on the large, plush bed, the letter in his hand looking as if it may fall apart with the slightest pressure. Catie was pacing beside the bed, her hands twisting around each other incesantly. "Your grandmother?"
"I know," Catie mumbled. "I know, it's crazy! And I wouldn't believe it if I didn't see it with my own eyes. But, it's undeniable. She even mentions my grandfather's name! I mean, that's not a coincidence! It can't be!"
"Have you shown it to your father yet?" Ethan watched her, picking up her nervous energy, his heart racing along with hers now.
"No. I... I... I just don't know what I would say to him. How... what do you say in this situation? 'By the way, dad, I found a note today from the mother you never knew'? I mean, he'd freak out on me. Well, actually I don't know how he'd react, but I know how I reacted and I freaked out!" Catie dropped onto the bed, curling up so that she faced her partner. "When I was little, I asked my grandfather why I didn't have a grandmother like most other kids. He told me that she had been taken by very bad men because they wanted to steal my father, and she had died to protect him, and to protect all of us," she sighed, her nostrils flaring against her flood of emotions. "Ethan," her clear green eyes pierced him, "today I sat in the room where my grandmother was kept, and probibly tortured, for six months! The chair I sat in... it could have been the exact spot that she sat and prayed for my grandfather to rescue her. I was that close to her today!"
Ethan slid next to her, the letter placed between them as he wrapped her in his arms and held her against his chest. "Of all places for those files to be hidden. That's one hell of a coincidence."
"It isn't coincidence," she whispered. "Everything happens for a reason. My grandmother believed that with all her heart. Those files were hidden in that office so that I could go after them and I could, therefore, find her letter. It was her plea for life, Ethan. Her plea for my father's life. When I read it, I felt like I was there, with her, crying as I made my last effort to save my child, to make some kind of an impression on the world. It was like her last will and testiment. Like, she knew I would find it. Like, she had somehow chosen me, just me, to carry out her final wishes."
"Then do it," Ethan told her quietly, kissing her head. "Carry out her final wishes. Tell her son everything she wanted him to know. I can't imagine any other way of honoring her memory now. I can't imagine anything that would make your father, and your grandfather, happier." Catie looked up at him, uncertainty still trying to creep it's way in. "Do it for our child; the great-grandchild she wanted to see so badly," he ran his fingers lightly over her abdomen. "Do it so that our baby can know the amazing line of women that he, or she, came from."
Catie grimaced nervously, but then kissed Ethan quickly and sprang from their bed and out into the tunnel passageway.
There were footfalls on the metal staircase that led into the chamber. A heavy-footed man and a light-footed woman. Vincent smiled, proud that he was still able to distinguish such things. As he set his book down, he found his son and his granddaughter smiling warmly down at him. "Well, hello," he tipped his head, his smile tucked into the corners of his mouth. "Catherine!" he made a point of her name, as he always did, but she flinched away from it. "How are you feeling today?"
"Very fine, grandfather," she settled herself in the little wicker chair that had always been hers. "I recovered those files, detailing the plans and possibilities of constuction under the subways. They're out of the wrong hands, and I've given them to my friend, Andy at the D.A.'s office. He's promised to take care of them for us."
Vincent smiled fondly at her, taking her hand in his. "Catherine; always our little protector, aren't you? I'm very proud of you. You take after your father, you know. Doesn't she, Jacob?"
"I like to think so," Jacob settled himself in the chair across from Vincent. "Father," he unbuttoned his suit jacket, suddenly uncomfortable, "we're not here to tell you about the files."
"Oh?" Vincent glanced between the two, his silver mane shaking as his head moved.
"Grandfather," Catie cleared her throat and set her other hand on top of his, "I found something yesterday. Something... well, something of a miracle, really."
"You see, father," Jacob began expounding, "the building that is now the bank where Catie collected the files yesterday wasn't always a bank." Jacob loosened his tie as he continued. "I did some digging Above today, but I can't find anything on the previous owner accept that his name is a suprisingly obvious alias. However, thanks to Catherine, we're fairly certain that we know who the previous owner was."
"Are you two going to get to the punchline, or am I going to have to guess?" Vincent joked at their oddly somber expressions.
"Grandfather," Catie took over, "I was in that building yesterday, in a large office, searching for the files about the construction, and I found this," she nodded to her father, and he slowly reached out and handed the preciously folded paper to Vincent. "It was tucked under the carpet and half-hidden in the wall."
"It's a letter, father," Jacob explained as he watched Vincent's old fingers work open the fragile folds. "It's a letter to you and I. It's from-"
"Catherine..." Vincent sighed as he began reading, his hand immediately finding it's way to his mouth. "This isn't possible. This is just not possible," he whispered.
Catie sat silently, just as she had with her father, watching him read the old letter as he repeatedly denied it's credibility to himself. He was quiet mostly, but he chucked at a line here or there, and as he made his way toward the end, he swiftly began to cry. When he finished, he let the letter fall into his lap, tilting his head up, a hand covering his wet eyes. "Catherine?" he called in a whisper.
Catie sat perfectly still, glancing at her father, confused as to whether he was speaking to her, or the Catherine of the letter. When Jacob gave no answer, she leaned forward and tentatively answered. "Yes, grandfather?"
"Are you certain," his hand came away from his eyes and he was bearing down on her intensely now, "are you absolutely certain that this is not some sort of sick joke? Are you certain that this is real? Can you prove this to me?"
Catie took a moment before she answered him, forming her words with purpose. "I only know that yesterday afternoon, I went to the Prudential building on 23rd street. I know that I went searching for the files, and found this letter stuck underneath the carpet and covered with wallpaper. I know that it looks like it's been there for a very long time. And I know that it contains details about you that no one else could have known." She took a breath and closed her eyes for composure; Vincent had begun seeping with unashamed tears. "I believe, grandfather, that I found some lost piece of my grandmother yesterday. I believe that at somewhere around noon, I stood in the same room where Catherine Chandler stood forty-five years ago and waited, wondering when you would rescue her, or when they would finally kill her. That's all that I know."
Vincent sighed and dropped his head, his eyes skimming the soft pencil marks on the paper before him. "Jacob," his son sat rigid and wide-eyed, "this is your mother's handwritting. There's no mistaking it. This letter," he ran the pads of his thumb and forefinger against it lovingly, "was never meant for me. It was meant for you, Jacob. It was meant for the person who found it in the first place. You, Catherine. I appreciate your sharing it with me, but it is not for me to keep," Vincent handed the paper delicately to the young woman beside him.
"But, father..."
"Jacob," Vincent stopped him, their identical eyes meeting, "that letter was a message to you from your mother. It was the only way she knew how to tell you how much she loved you, and everything else she wished she could share with you in your life. I already know. I already know it, Jacob. Here," he clutched his heart, "and here," he touched his head. "Your mother's heart was my heart, Jacob. We knew each other beyond mere words. She didn't need to say anything to me. I already knew it. Keep it. Read it. Remember it. Read it to your child, Catherine. Read it to your grandchildren. That was all she wanted; for her family to know who she was, and how much she loved them, and what she had given to them. This letter, it was never for me."
Slowly Vincent captured Catie's head and kissed that sweet, honey-colored hair. With an obvious effort, he climbed to his feet and placed a warm, comforting hand on his son's shoulder. Then, he laborously made his way to his chamber, leaving his puzzled family behind him.
"Do you think he did it on purpose, dad?"
"I think that my father was a very troubled man, Catie. He was plagued with all sorts of ghosts and shadows of his past for as long as I can remember. But, I don't think anything haunted him more than the death of my mother."
"Did I do this to him? Did I push him to this, daddy?"
"No, my sweet. I believe that letter made very little difference at this stage of his life. Your grandfather lived with the knowledge that he had a promise to keep. When Caleb was born, he saw it as an opportunity to finally let himself go. He had promised my mother that he would one day tell her of her great-grandchildren. Once your son was born, he knew he had fullfilled his promise, and he started to let go."
"I hate knowing that he spent half of his life waiting to die."
"Then don't think about it that way. Think about it as if he were simply waiting to go home."
"It really is beautiful, isn't it? The sunset?"
"It is."
"Daddy, do you think he had this in his will the whole time?"
"My father had amazing foresight, Catie, but I think this really had to do with that message you shared with him."
"... A ceremony at sunset. Perfectly fitting. Catherine would have liked it."
"I would agree, sweet. And I'll bet their both standing here with us, now, watching the two worlds touch."
The large presence moved away from Catie, a step and then two ahead of her, toward the setting sun. A smile graced his entire being. All was well with the world, an incredible serenity swirling in the orange light. A hand suddenly closed around his, and he looked down to find the soft features of the most beautful creature he had, and ever would, lay eyes on. He only stared, taking her in for the moment, her small warm hand in his was an indescribable comfort.
"So," her smile radiated off of her, "you've come for me."
"I'll always come for you."
"Walk with me?" she asked, gesturing to the glow of the setting sun. "Tell me of your adventures!"
"Oh, but where to begin..." he followed her into the invisible distance.
I pray she finds your light
And holds it in her heart
As darkness falls each night
Remind her where you are
Every mother's prayer
Every child knows
Need to find a place
Guide her with your grace
Give her faith so she'll be safe
Lead her to a place
Guide her with your grace
To a place where she'll be safe
**Mother's Prayer by Celine Dion


text:
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|Extended warranty + 1 year £82.60 Extended warranty + 1 year
This compact electret condenser microphone brings new possibilities in sound reproduction is made for professional use. It can be used with professional series camcorder HDV, DVCAM or XDCAM. Among the great advantages include excellent sensitivity, low inherent noise and extreme durability.
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Mono electret condenser microphone
Frequency response:
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Directional characteristics:
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DB -28 +/- 3 db
Output impedance:
200 ohms ± 20%, Balanced
Dynamic range:
111 db
Signal to Noise Ratio:
78 db
Custom noise:
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Maximum input sound pressure level:
127 db SPL
External, DC 48 V +/- 4 V
20 × 250 mm
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Specifications can be changed without notice. Images are for illustrative purposes only. Retail Price (MSRP) means the manufacturer's/supplier's suggested retail price.
Link to Manufacturer's Website: Enter link to the external review


text:
Melissa & Earl August 05,2006
by ionuphotos
About the Book
A collection of photos from the marriage of Earl and Melissa Lang in the BC mountains on Aug.05,2006.
Author website
Features & Details
- Category Wedding
Large Format Landscape, 13×11 in, 33×28 cm
48 Pages
- Publish Date Aug 06, 2008
- Language English
- Tags Canada, mountains, photographer
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text:
This topic is for discussing childcare options. If you want to advertise, please use your Local site.
Sorry Mrs Judgeypants - look away! Its confessions of a childminder(37 Posts)
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I'm picking through my annoying but not so critical problems today and came across this one I'd like to get working.
Periodically our remote users will be prompted to change their domain password when using the NetExtender SSL-VPN client.
This is normal as their password is due to change on the domain or has expired. However when they try to do this, it always fails, even for me.
If I just try to change my password while I'm logged on to the VPN client, it works fine.
Any ideas?
Jan 31, 2012 at 3:09 UTC
So I think I have found the solution. In a completely different section than the setup for LDAP, I see this little hover over help message. Read the last paragraph.
14 Replies
Jan 25, 2012 at 10:22 UTC
I would assume it works when signed in cause you are on the network and actually working with AD.
When Extender is trying to commuicate with AD through the Sonicwall. I think there is a setting to sync with AD vs interact with AD.
Jan 25, 2012 at 10:38 UTC
Tim I looked around and can't find any setting to sync with AD. I see the settings for authentication through LDAP using MS AD and that is setup and working normally.
Jan 25, 2012 at 2:39 UTC
Are you using SSO?
Are you using LDAP + local or just LDAP for authentication?
Jan 25, 2012 at 3:31 UTC
We're using LDAP + Local
Jan 26, 2012 at 7:15 UTC
Wonder if we should just stick with LDAP since we're only using the SSL-VPN.
I think I'm going to call Sonicwall and see what they have to say about it.
Jan 26, 2012 at 8:26 UTC
I haven't set it up yet but plan to do what you are doing. I think you may need to have SSO setup for it to work the way you want to. Let me know what you find out.
Jan 27, 2012 at 3:12 UTC
Have you tried rolling back to just LDAP?
Seems like LDAP + Local would cause confusion..
Let us know what worked for you..
Jan 31, 2012 at 3:09 UTC
So I think I have found the solution. In a completely different section than the setup for LDAP, I see this little hover over help message. Read the last paragraph.
Feb 2, 2012 at 8:46 UTC
Well my horrible experience with Sonicwall support continues. I opened a ticket Monday with support and got called at 3PM EST yesterday.
The gentleman that called me barely spoke English and barely understood my English. To top it off the phone connection sounded like he was in the middle of the ocean.
He never understood what I was saying and kept insisting it was an AD problem since he thought I was saying AD was prompting for the change when it was the NetExtender app that was asking for the password change.
Oh well...
Feb 2, 2012 at 9:44 UTC
I know your pain. I HATE calling SonicWALL support, for that very reason, they don't speak clear English.
Feb 10, 2012 at 7:59 UTC
Such an epic SonicWALL fail all around. I understand that folks across the seas need jobs too but are they really successfully doing their job if they cannot communicate with their customer successfully?
I'm going to throw some helpful posts out there folks but this isn't solved.
Mar 26, 2012 at 5:38 UTC
I have had that happen as well and usually can call back a little later to talk to someone that speaks English a little more fluently, or demand that I talk to someone that speaks it a little more fluently.
I have set this up in a lab environment only (at home on my tz210 with domain controllers setup on VMware Workstation). Here is the article I used to get TLS working with the Sonicwall which then allowed me to change my password from the NetExtender client. I am getting ready to do this in the production environment.
It does involve setting up your own CA which isn't really all that complicated but its another thing to manage.
Apr 16, 2012 at 10:01 UTC
OK folks I finally figured this out!!!!!!!!!!
Man o man...
2 things were incorrect in my environment:
1. The SonicWALL user was not an administrator so it had no authority to change passwords.
2. This KB: https:/
Check the following security settings on the "Domain Controller Security Settings" in the Active Directory:
Domain Member: Digitally encrypt secure channel data (when possible): Enabled
Domain Member: Digitally sign secure channel data (when possible): Enabled
Network Security: LDAP client signing requirement: Negotiate signing
Domain Controller: LDAP Server signing requirement: None
After making the above changes (if any), update the group policy by running 'gpupdate / force' command
As soon as I made the GPO changes after researching them the password change magically worked!
What a pain in the a$$. I asked them to update the help documentation to point to that KB but we'll see what happens.
Apr 30, 2013 at 3:16 UTC
So if the user goes ahead and updates the password through NetExtender, I am assuming that will also update his password on the network, and the next time he logs in he will need to use that updated password?
I have a user getting the same prompt to update his password although his current password is not expired. As a matter of fact, I just updated his password yesterday so it should be valid for a while.
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text: To get from Montevarchi a Trentola-Ducenta by train you can catch one of the 40 trains that everyday travel between Montevarchi-Terranuova and S. Marcellino-Frignano, the closest station to the city. The first train to S. Marcellino-Frignano leaves from Montevarchi-Terranuova at 02:18 and the last train departs at 22:04. The trip by train from Montevarchi-Terranuova to S. Marcellino-Frignano last on average 02:31 travelling with high-speed trains (Frecciarossa or Italotreno) If you choose a local train the trip lasts on average 03:05 hours. Tickets price depend on the departure time and train type, the most economic train ticket available this week is from € 46 to € 69. Prices for next month range from € 28 to € 49. The most economic train is ItaloTreno () that departs at 12:33 and arrives in S. Marcellino-Frignano at 15:05 at € 28. On Wanderio you can compare all schedules, fares and railway companies to S. Marcellino-Frignano and book the best itinerary starting from Montevarchi-Terranuova.


text:
|Performing Arts
Contemporary drama in a rural setting
November 22nd, 2015
Episode 347 of 870 episodes
Helen's not driving at the moment and has to beg a lift to the Bridge Farm shop from Pip, who tells Helen about Ruth's planned trip to New Zealand. Helen would get away from it all like a shot, but Pip reminds her she has a big shop opening to plan for. Oh yes, remembers Helen. Helen half jokes with Rob that she won't be a prisoner at home. Helen seems rather dizzy, pale and tired and Rob fusses over her. Back at home, Rob makes Helen dinner (he and Henry have eaten already) but Helen can't eat it. Rob points out that she has overdone things and Helen takes herself off to bed. Ruth has it out with Pip and David over feeling shut out. David's reeling from Ruth's announcement on Friday. He tells Jill that Ruth's turning her back on him - he wouldn't spring something like this on her. Jill advises that David takes Ruth at her word and appreciates how the last year has turned her world upside down. David should be philosophical and accept that Ruth's going to New Zealand - as Jill accepts that Stir Up Sunday isn't necessarily the tradition it was with the kids growing up. David reluctantly agrees.
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Welcome to the Brain Training Podcast, the daily audio workout for your head. In this podcast we have two games for you, each with three rounds which get progressively harder. To enjoy the full experience, relax, and avoid distractions whilst you listen.


text:
What is the PotCoin Seed Bank – F.A.Q.
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The PotCoin Seed Bank is a simple and easy way to turn your PotCoin into real cannabis seeds.
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PotCoin has partnered with leading cannabis seed producers to offer this amazing opportunity to the PotCoin community and cannabis enthusiasts. Based on our agreement with seed producers PotCoin will use generated stake returns to pay for and distribute seeds to members of the PotCoin Seed Bank. The more PotCoins you stake the more seeds you earn.
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Fill out the form below click join and you will receive a confirmation e-mail as well as a deposit address for the Seed Bank.


text:
German Dating Customs?
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text:
Bangalore, Sep 24: Riding high on a comfortable win over Dolphins in their last encounter, Chennai Super Kings will look to keep the momentum going when they lock horns with Lahore Lions in the Champions League T20 here on Thursday.
Schedule; Points Table; Photos; Results
Captain Mahendra Singh Dhoni would like to see the in-form Suresh Raina, who blazed his way to a 43-ball 90 against Dolphins, to continue with his blitzkrieg and make Chennai's case stronger to storm into the knockout stage.
Chennai, who blazed their way to set an imposing 242 for six in 20 overs against Dolphins, would love to exploit the placid Chinnaswamy pitch against the Pakistani side. (Raina sets records in Bangalore)
The West Indian and Chennai T20 opener Dwayne Smith, who had a pretty good season last IPL, would like to make amends as he had failed to make any impression with his bat in the last two matches.
However, Brendon McCullum found his groove by hammering a quickfire 49, which gave a launching pad for Raina and Co. To post a massive total for Dolphins to chase. (Video: 'Superman' McCullum defies gravity)
McCullum and Smith would also look to join the party after failing to contribute much in earlier matches.
Having made amends in batting, Chennai also impressed with their bowling efforts as Mohit Sharma (4 for 41), Ashish Nehra (2 for 42) and Dwayne Bravo (2 for 17), were among the wickets. R Ashwin, who picked a wicket in the last match, would be keen to make a dent in the Lions' camp.
Lahore's Saad Nasim, Captain Mohammed Hafeez, Ahmed Shezad and Umar Akmal have been impressive with the bat. They struck 59 and 40 respectively in the match against Kolkata.
Akmal had also impressed in the game against Mumbai Indians in the qualifying round. Hafeez has also been striking well with the bat, scoring a superb 67-run knock in the match against Southern Express.
Lahore bowlers Aizaz Cheema, Wahab Riaz and Imran Ali have been successful in the qualifying matches. But the team will need to put up a very strong all-round performance to make it difficult for the seasoned Chennai side.
Chennai Super Kings: MS Dhoni (captain and wicketkeeper) Dwayne Smith, Brendon McCullum, Suresh Raina, Faf du Plessis, Dwayne Bravo, Ravindra Jadeja, Ravichandran Ashwin, Mohit Sharma, Ashish Nehra, Ishwar Pandey, Pawan Negi, John Hastings, Samuel Badree, Mithun Manhas
Lahore Lions: Mohammad Hafeez (captain), Umar Siddiq, Ahmed Shehzad, Nasir Jamshed, Saad Nasim, Umar Akmal (wicketkeeper), Wahab Riaz, Asif Raza, Mustafa Iqbal, Adnan Rasool, Aizaz Cheema, Mohammad Saeed, Ali Manzoor, Agha Salman, Imran Ali.
Match starts 8 PM IST
Live scores here
Live streaming on host broadcaster


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How rich is Manny Pacquiao?
Manny Pacquiao Net Worth:
$190 Million
Follow Manny Pacquiao:
Manny Pacquiao net worth, wiki & biography:
With $100 million amount net worth, Manny Pacquiao name dhimsle as one of the richest professional boxers in the world. According to Forbes Manny Pacquiao annual salary is about $40 million in 2009. Latter, Pacquiao amount salary is increased significantly to $42 million and stuck still at the amount of $67 million in last 2012. Sport or being the professional boxer is the pivotal money source or Manny Pacquiao. Manny Pacquiao Net Worth Despite receiving payment and bonuses from the boxing, this man also received a lot of money from the endorsement too.
Manny Pacquiao Net Worth $100 Million
The list of companies that endorsed Manny Pacquiao such as Hewlett-Packard, Nike, San Miguel, Hennessy, and Monster Energy. Manny Pacquiao also gathered a lot of assets that won't make him worried in the future. It was reported that Manny Pacquiao has $9 million luxury houses in Forbes Park, Philippines and 2.16 million houses in Hancock Park, Los Angeles. Manny Pacquiao may receive money from the boxing and it made his pivotal money source. Manny Pacquiao reported receiving about $25million when fights against Juan Manuel Marquez on 11 December 2011. He also reported paying more than $22 million when the fight against Miguel Cotto on 14 November 2009. Manny Pacquiao most historical championship probably when he should fight against Oscar De La Hoya on 6 December 2008 that he was paid over $11 million.
Emmanuel "Manny" Dapidran Pacquiao who was born in Kibawe, Bukidnon, Philippines on December 17, 1978 is a Filipino professional boxer. Despite being a Filipino professional boxer, currently Manny Pacquiao also best known as the politician too. When moved to Manila at the age of 14, Manny Pacquiao started boxing and made the Philippine national amateur boxing team. He describes that due struggling to live in Manila and spent almost his entire time in street, boxing is the only way to survive. In 1995, after the death of his friend, Manny Pacquiao pursued the professional boxing career right when he is still 16 years old. Pacquiao started to steal peoples attention when he was winning after knock out Chatchai Sasakul in the eighth round. In super bantamweight class, Pacquiao makes people shocked after winning against Lehlohonolo Ledwaba at the game that was held in the MGM Grand Garden Arena, in Las Vegas, Nevada. Along his career, Manny Pacquiao won several champions from different boxing division such as Light Welterweight, Lightweight, Super Featherweight, Featherweight, Super Bantamweight, and Flyweight.


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The second trimester is typically a heck of a lot more fun than the first trimester. You tend to feel better, love how you look, and aren't yet experiencing many unpleasant symptoms of the end of pregnancy. That doesn't mean that you should blow off all the things you need to plan for in the coming months. This list will help keep you on track!

 Register for baby items. Your baby shower registry is not only a place for people to look for gifts to purchase as you welcome your new baby, but it is a great organizing tool. You can use it to hold items that you want to compare, either for other items or by price shopping. It can also be a great way to start looking at a variety of the items made for babies. As a first time parent, it can be difficult and overwhelming to look at the plethora of items available. Rest assured, you do not need most of the items that are out there. Consider registering for the basics and nothing more. You might also want to consider alternatives to the traditional baby registry. Think outside of the big box store box, and look for registries for cloth diapers, birth and postpartum doulas, or other items that you might use. They don't even have to be specifically baby related – think meal services for postpartum dinners.

 Get educated on birth topics. While you might feel like you are putting the horse before the cart by thinking about birth at this point in pregnancy, many of the options that you have will take conversations with your place of birth, your practitioner, and your support team. The sooner you know about all of the options available to you, you can figure out which options are right for you and your baby. It is also important to register for your childbirth class of choice early. Believe it or not, these book early. So take the time to look at the childbirth classes available in your area and sign up for the one that works the best for you.

 Get your baby's nursery going. Don't laugh but if you decide to order baby furniture for your baby, it can take a whole trimester to come in. That can cause a lot of stress when you are nine months pregnancy and informed that there is a delay in the production of the furniture. I also think that having a room or area ready for baby earlier in pregnancy can give you a place to go and relax as you think about your impending parenthood. You will also have more physical stamina to help at this point in pregnancy. Waiting may make it more difficult for you to participate.

 Take a vacation. Some people call these a babymoon. Basically, it is a last vacation before the baby is born. Some people try to live it up and go crazy – an overseas vacation, something on the beach, some wild travel plans abroad. That said, there are plenty who stick closer to home to enjoy a weekend away, or even a staycation. Whatever you decide on – get away and remember to have a blast. Be sure you focus on your relationship and not deal only with the pregnancy.


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Our ready-to-go templates, our point-and-click designer and our helpful customer support team are a few of the things our users love most about ShortStack.
Take a look at what they have to say.
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text: The GTEM!™ Test Cell is based on experience, not experimentation. Originally developed in the EMC Baden (Switzerland) labs of ABB, the cell has been accepted in the EMC community for more than 10 years, and is field proven daily at more than 400 installations worldwide. Measurements made with a GTEM!™ Test Cell are accepted for final compliance demonstration by the FCC for Part 15 & 18 radiated emissions testing, and comply with IEC 61000-4-3 Annex D for immunity testing. The GTEM!™'s unique tapered shape, offset septum, resistive termination network and absorber- lined back wall remove performance limitations of TEM cells and boxy enclosures. Electromagnetic wave and RF current termination are smooth and controlled. Field uniformity is +/- 3 dB up to 1 GHz, and +/-4 dB above 1 GHz.


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Raman, H. and Platz, G.J. and Chalmers, K.J. and Raman, R. and Read, B.J. and Barr, A.R. and Moody, D.B. (2003) Mapping of genomic regions associated with net form of netblotch resistance in barley. Australian Journal of Agricultural Research, 54 (12). pp. 1359-1367.
Article Link(s): http://dx.doi.org/10.1071/AR03026
Publisher URL: http://www.publish.csiro.au
Quantitative trait loci (QTLs) associated with resistance to net blotch and their chromosomal locations were determined from analyses of doubled haploid progeny of Alexis/Sloop, Arapiles/Franklin, Sloop/Halcyon, and recombinant inbred lines of Sloop-sib/Alexis. Five QTLs on chromosomes 2H, 3H, and 4H were found to be associated with seedling resistance to the net form of net blotch. In Arapiles/Franklin and Alexis/Sloop populations, 4 significant QTLs explaining 9–17% of the variation in net blotch resistance were detected on 2H and 3H. A major locus, QRpts4L accounting for 64% of the variation in infection type, was detected on 4H in the Sloop/Halcyon population.
In Sloop/Halcyon, 2 microsatellite markers, EBmac0906 and GMS089, and AFLP marker P13/M50-108, co-segregated and detected maximum variability for net blotch resistance as revealed by bootstrap analysis. EBmac0906 and Bmac0181 were validated in F2 progeny of an Ant29/Halcyon population and reliably predicted phenotypes of 93% of lines resistant and susceptible to net blotch. These markers may be used within breeding programs to select alleles favourable for net blotch resistance derived from Halcyon.
|Item Type:
|Corporate Creators:
|Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science
|Business groups:
|Agri-Science, Crop and Food Science
|Additional Information:
|Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher's website.
|Pyrenophora teres f. teres; QTL; seedling resistance; validation.
|Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Science > Biology > Genetics > Quantitative genetics (esp. Quantitative Trait Loci (QTL) articles)
Plant pests and diseases > Individual or types of plants or trees > Barley
|Deposited On:
|08 Jun 2004
|Last Modified:
|08 Jun 2015 15:50
Repository Staff Only: item control page


text:
Thursday, August 18, 2016
Off the Shoulder Tassel Top
32 comments:
Such a cute top! Love how you styled it with neutral accessories.ReplyDelete
~ xo Sheree
Posh Classy Mom
Such and adorable and festive top. Love the shoes as well.ReplyDelete
The top is still so gorgeous!!! love all your looks!!ReplyDelete
enjoy your day!
❥ Vicky | The Golden Bun - Instagram TGB-
i kinda need this top.... ok.... i need it a lot!!!!ReplyDelete
Super cute look! I am loving those wedges and that bag! Fabulous! As excited as I am for Fall, I am trying to enjoy my summer clothes for now, too. Because there is no sign of cooler weather in the near future!ReplyDelete
Such a cute outfit! I love that tote!ReplyDelete
this is such a cute outfit the top is so cheap too RIGHT UP MY ALLY love itReplyDelete
Life is just Rosie
I love your top and bag! So adorable!ReplyDelete
Love that top and those shoes are too cute.ReplyDelete
Those legs girl!! stunning!!ReplyDelete
i love this top! you look so good and dang girl, your legs!ReplyDelete
Love the tassel trends.ReplyDelete
This top is adorable! love this lookReplyDelete
Love the shoes, and the bag.ReplyDelete
xo, Emily
Such a gorgeous look, I love that top on you!ReplyDelete
Super cute tassels! I also LOVE that bag!ReplyDelete
You look too cute! Love that top!ReplyDelete
Cute top! Perfect for the end of summer!ReplyDelete
Love that top! What a fun linkup!ReplyDelete
That top is so cute on you! I'm obsessed.ReplyDelete
xoxo, Jenny
This is so cute!! Love your shoes too!ReplyDelete
Absolutely adorable. So fun!!ReplyDelete
Such a fun top! I love everything tassels for summer!ReplyDelete
xox Tess | Sequins are the New Black
This little tassel top is so cute! It would be perfect to wear at the beach.ReplyDelete
Amanda || www.fortheloveofglitter.com
This look is so cute! I adore that top. Those tassels are adorable.ReplyDelete
You look adorable! Have a great weekend <3ReplyDelete
Edye | Http://gracefulcoffee.wordpress.com
Love that bag so much!! you look amazing. xoReplyDelete
So effortlessly chic! Gorgeous.ReplyDelete
How adorable is this pom top?! Entire outfit is so cute and your tan looks amazing!ReplyDelete
Very happy because presented with the information that is so very helpful thanks.ReplyDelete
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Such a cute top. Love the tassels!ReplyDelete
Greta | www.gretahollar.com
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Uganda is the New 'It' Tourist Destination
Having been to The Serena Hotel in Kampala, Uganda, and eaten some of the most delicious East African cuisine, I can tell you that while my Mom was an excellent cook (Hi, Mom!), she has nothing on a master chef. In Uganda, there aren't many master chefs, in fact most of the restaurants are locally-owned establishments where Mom actually is cooking, but The Serena Hotel is a bit different. Beneath The Serena's rose-colored towers high above downtown Kampala lies a restaurant that indulges all 5 senses, and maybe the 6th sense too. The sense of wonder!
The flagship restaurant, The Pearl of Africa, is stunning! And the views over the terraced lawn and pools are immaculate. In fact with the cascades of a 7 meter waterfall flowing into the largest pool complex in all of Uganda, The Serena Hotel in Kampala even outdoes itself. Why itself, you ask? Well The Serena was already the finest establishment around, but just last year completed renovations to make it a premier destination in not just Africa, but the entire world.
Given the abundance of activities in Uganda (and shhhh! the prices are much lower than in other parts of Africa), including incredible big game safaris, gorilla trekking, the white sand beaches of Lake Victoria, and amazing freshwater sport fishing, I can't understand why more travelers aren't flocking to this burgeoning tourist mecca. The reason: It's still not quite discovered, so you can be among the first to go and enjoy everything Uganda has to offer. When you do, stay at The Serena Hotel in Kampala, and let them take care of the rest.
Belmond Safaris
Okavango Delta, Botswana
Belmond offers the best luxury safaris in Botswana. Stay in authentic African surroundings, as local experts lead you to wildlife experiences other travellers rarely access: this is Africa at its most revealing, enjoyable and... more >>
Ngala Game Reserve
Kruger National Park, South Africa
Ngala, meaning "Lion" in Shangaan, offers incredible opportunities to see several prides of lion in the wild, freely roaming this spectacular game reserve. Ngala Private Game Reserve, South Africa is a sanctuary for... more >>
Kirawira Luxury Tented Camp
Arusha, Tanzania
The most exclusive of the Serena Group's many safari venues, and a member of the Small Luxury Hotels of the World group, Kirawira Tented Camp offers the epitome of classic 'Out of Africa' safari camp luxury. Styled to replicate... more >>
Bushmans Kloof Wilderness Reserve
Western Cape, South Africa
At the foothills of the Cederberg Mountains only 270km from Cape Town, Bushmans Kloof Wilderness Reserve & Wellness Retreat is an ecological oasis within a roughly hewn and magical land. This spiritual retreat is a haven of... more >>
Singita Ebony Lodge
Mpumalanga, South Africa
As the original Singita lodge, Ebony stands steadfast amongst enormous trees on the banks of the Sand River in the heart of South Africa's 'big cat country.' A blend of European heritage and African boldness welcomes visitors... more >>


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Improving margins helped data-storage vendor EMC Corp. increase net income by 58 percent in its third quarter, the company reported Tuesday morning.
Shares of EMC (NASDAQ: EMC) gained 4 cents, or 0.2 percent, to 20.84. Not much of a bounce from a strong earnings report, but given that the Nasdaq and most major tech stocks took a beating through the entire trading session, EMC investors probably should be relieved.
EMC's third-quarter net income was $472.5 million, or 22 cents per share, up from $298.2 million, or 14 cents per share, in the third quarter of 2009. Excluding one-time costs, EMC reported a net profit of 30 cents per share -- right in line with consensus estimates -- versus 23 cents per share a year ago.
Revenue for Q3 was a record $4.2 billion, up 20 percent from last year's $3.5 billion. The Hopkinton, Mass.-based company's product revenue rose 22 percent, while revenue from services gained 17 percent.
EMC's operating margin nearly doubled to 15.4 percent from 8.7 percent in last year's third quarter. The company also reported record year-to-date operating cash flow and free cash flow of $3.0 billion and $2.2 billion, up 31 percent and 22 percent, respectively, from the year-ago period. EMC ended Q3 with $10.5 billion in cash and investments.
In a statement, EMC Chairman and CEO Joe Tucci cited the company's success in building a cloud business. "Customers are embracing EMC in increasing numbers as a trusted partner for their cloud computing build-outs," he said.
The company also upped its annual earnings and revenue to $1.25 per share on sales of $16.9 billion. In July, EMC forecast full-year earnings of $1.18 per share and revenue of at least $16.5 billion. Analysts have been forecasting net income of $1.21 a share on revenue of $16.7 billion.


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Capitalist has tightened his grip on Saturday's Magic Millions Classic after drawing barrier two as his closest rivals in betting drew the outside.
The Written Tycoon colt continues to do everything right. He has the perfect record with the Breeders Plate and Wyong Magic Millions wins, and a perfect draw.
He completed his preparation with a gallop at Randwick on Tuesday morning before drawing an inside gate, as did stablemate Niccolance (gate nine), while Mooshakissa was the only disappointment for the Peter and Paul Snowden camp coming up with gate 18.
"He did actually what we wanted to see this morning and the barrier is good. I'm not one for worrying about barriers but that makes it easier," Peter Snowden said.
Capitalist was $1.75 to $1.65 with TAB fixed odds, with Nikitas, which is next to the favourite in barrier three, and unbeaten filly Zelady's Night Out (gate 19) at $9.50, while seven of the field of 21 are $101 and better.
"There were only four chances before the draw to my mind and a couple of them drew out," TAB's Glenn Munsie said.
"Capitalist drew the gate, has the form and is the one to beat, but you are going to have to take short odds.
"I think Zelady's Night Out and Slumber Party had their chances hindered by the draw, while Nikitas and Niccolance have some hope."
Zelady's Night Out's trainer Tony Gollan had mixed feelings about the draw as his other hope Just Orm came up with gate eight.
He hinted that Zelady's Night Out could go forward from her wide barrier.
"I'll tell you after they've gone a furlong on Saturday how the draw is," Gollan said.
"She has got brilliant gate speed and there are lot of things that can happen. You prefer not to draw 19 but there is not much we can do about it, we will just make the most of it.
"Just Orm has drawn perfectly in eight and he's just going to get the right run just off the speed and be ready to ping at the right time."


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Glen (Character)
from Hoodwinked! (2005)
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- Hoodwinked! (2005) Played by Tony Leech
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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Jeff Turner <je...@apache.org>
|Re: Forrest and the NDS (Re: [RT] Moving towards a new documentation system)
|Sun, 12 Oct 2003 12:31:45 GMT
On Sun, Oct 12, 2003 at 12:40:39PM +0200, Stefano Mazzocchi wrote: ... > In case something is not clear, I'll be very happy to explain it. My main question is: to users, how is this system functionally different from a Wiki? Eg, a good one that has 'related pages' inferred from the page's content: http://wiki.opensymphony.com/space/WebWork+2+Components And a naive question: wouldn't Subversion be a better backend than this JSR170 thing? Then developers could store xdocs in the same repository as code, and could check in updates with standard svn tools (including IDEs). Thanks, --Jeff > -- > Stefano. >
|View raw message


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The main objective of this project - to create an embeddable version of DOSBox, which may relatively easily run on really low-cost hardware. Like modern cheap microcontrollers.
This version supports only (de-facto) standard PC hardware from mid-90s: S3 SVGA for Video and SBlaster for Audio.
Another part of the project is a software library, which allows you to create safe multithreaded multi-instanced DOS VMs environment for your own project.
More information can be obtained through SVN commit history and plain text description files inside the code tree.
Other Useful Business Software
User Reviews
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text:
Most men done 50 more often than not go through from expanded ductless gland or benign endocrine hyperlasia (BPH). Until now, no learned profession study can accustom why many men have enlarged endocrine. After you have been diagnosed next to BPH, you keep up to in performance beside the symptoms and consequent uncomfortableness. Even nonetheless it is not a life daunting condition, you improved wish use if you have any of the enlarged prostate symptoms.
The magnitude of a majority endocrine gland secretor should be as big as a walnut. However, as you manage mid forties, the secretor tends to get larger. It could be because of the hormonal inequality.
You may fight various symptoms of BPH. Most of the signs are cognate to body waste technical hitches. For example, you tend to take home a lot of application to urinate and you call round the lavatory more frequently, particularly at dark. Other signs may count piss leakage, fear of an unemptied bladder, and interrupted spill of excretory product.Post ads:
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You may undertake much weighty symptoms such as evacuation obstruction, nausea, support pain, existence of body fluid in the urine, and frenzy.
Swollen prostate can organize to further hindrance if you don't aliment it. It causes physiological condition by impeding your kidneys next to excrement. In such as cases, acute excrement retention can begin. It may even refine into confirmed excretory organ disease, urinary organ harm or infection, sac damage, and vesica stones.
To coping next to the symptoms of enlarged prostate, stop by your medical doctor more often than not for a ductless gland checkup, peculiarly if you are in your time of life. Don't feel humiliated as BPH is a standard procedure of old. Make positive you locomote the doctor's suggestion on treatment for endocrine. Some medications such as alpha-blockers and finasteride can meliorate the symptoms of BPH.Post ads:
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You may want to run after an secondary remedy. However, you condition to cognise that not all flavorer supplements are accredited by the Food and Drug Administration (FDA) specially saw palmetto, a common rectification for endocrine gland complications. They may not support at all.
Enlargement of the ductless gland can be a curse in your beingness. However, you can unmoving insight treatments for it. The go-to-meeting line of accomplishment is to want a nonrecreational persuasion from a urologist.


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Saturday, January 2, 2016
Disney Hasn't Forgotten About Star Wars 1313
7 comments:
dear lighter side of the force.......stop posting videos only. do some actual work and write articlesReplyDeleteReplies
I second that. The videos suck.Delete
Agreed. Love the content, would rather read it. Not always convenient to have to listen to audio. Thanks!ReplyDelete
Unfollowing because can't sit thru your vids, can you just write it?!?ReplyDelete
It's bad enough that every article turns out to be bullshit click bait with a video embedded, but does there have to be shitty dubstep in the background?ReplyDelete
Keep on posting only videos , I love how it irritates idiots. This site only posts videos. If that is not your thing unfollow them , they won't care.ReplyDelete
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text:
Airborne to be wild.
Set in a bustling bird city on the edge of the majestic Victoria Falls, "Zambezia" is the story of Kai - a naïve, but high-spirited young falcon who travels to the bird city of "Zambezia" where he discovers the truth about his origins and, in defending the city, learns how to be part of a community
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It was the news Brisbane basketball fans had wanted to hear since the Bullets shuffled off the court in 2008, heads down, waiting for a white knight to save their crumbling franchise.
The cavalry never arrived, although a few phantom horses were saddled up and ridden around in circles. Now, six years later, salvation is at hand in the form of a proclamation from the NBL that Brisbane can be guaranteed a team for the 2015/16 NBL campaign.
Whether that team is a reprise of the Bullets remains to be seen. The yellow, blue and white singlet remains an iconic sporting brand in the River City but those behind the club's potential return must prove their worth against rival consortiums.
And if none can fulfil the NBL's criteria by the end of next month, the governing body will enter a team of its own, much like the A-League has done with the wildly successful Western Sydney Wanderers.
NBL chief executive Fraser Neill was in Brisbane over the weekend for the pre-season blitz at Auchenflower and left convinced the Queensland capital was ready to embrace another pro basketball outfit.
But he said he had no preference as to whether that was the Bullets, a foundation NBL franchise from the inaugural 1979 season, or a fresh team on the scene.
"There will be a team in Brisbane, whether it be through one of the consortia putting together a bid or whether it's us running the team, similar to the Wanderers. It's happening," Neill said.
"We know there are bids being put together for a team. We're also doing our own work internally. Whatever it is, we're just going to punch through.
"I'd hope there is some fan input as to whether they want back the Bullets or they want a fresh start. I'm absolutely open to whatever works best for the team."
In the absence of an NBL side, the Brisbane basketball community has at times struggled to front under a united banner in the push to be reinstated to the league. But any group with designs on a licence needs to lace its hightops in a hurry.
"We're hoping to get bids in shortly. What we said to them is make sure you get it right. Don't give us 80 per cent of a bid, give us 100 per cent of a bid. Having said that, we need it by the end of next month at the very, very latest," Neill said.
Talk of any return of a team in Brisbane is quickly followed by speculation as to what venue would host games. The Bullets finished their life at the Brisbane Convention Centre at South Bank but that may be a costly option.
"I know there's been a lot of discussion about what venue would work best. The venue deal is fundamental to the success of a team," Neill said.
"They've gone through and looked at all the options. That's one thing they need to tie down before the come to us with the final proposal."


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Ratings: IMDB: N/A Metascore: N/A RT: N/A
Released: June 30, 2015
Runtime: 95 mins
Genres: Action Comedy Crime
Actors: Vinnie Jones
Rivers 9 ( 2015 )
Watch And Download Full Movies Online 100% Free
Rivers 9: When a Native American casino controlled by the mob begins to destroy a close-knit rural town, a band of lovable low-tech misfits hatch a plan to beat them at their
own game by pulling off an audacious casino heist.
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Released: December 4, 2014
Pretty secretary Donna Bell could call an early day because her boss, Mr. Lang let her home. But she stays in the office nevertheless, referring to "some more work" to do. The truth is that she is too horny to drive home. She starts to masturbate but then Mr. Lang comes back for something… catching her in action. The hot little secretary soon finds company.