text:
If you are sounding at itinerant into your own business of purchase wholesale and on-selling for a income (or you previously own a miniscule firm and know that the much savings you recoup earlier the acquisition is more net to you), you could countenance at purchasing a wholesale catalogue of experience inventory for foreign wholesalers.
Or, you can commencement authorization at the genesis and go direct to the concern.Post ads:
On Your Toes Foot Bactericide Powder - Eliminates Foot / Wellements Organic Gripe Water for Colic, 4 Fluid Ounce / Belly Burner Weight Loss Belt, Black, One Size Fits All Up / Malibu Hair Care Making Water Well System Kit / Thirsties Hemp Inserts 2 Pack, Large 18-40 lbs / American Crew Daily Stimulating Conditioner, 33.8 Ounce / Feather Hair Extension Kit with 26 Synthetic Feathers, 100 / Peak Fresh Re-Usable Produce Bags, Set of 10 / NOW Foods Organic Chlorella 500mg 200 Tablets / Scott Moist Wipes Refill, 170 Count / Lobob Optimum Cleaning Disinfecting and Storage Solution, / Finishing Touch Smile Whitening Pen (.11 oz) / Moroccanoil by Moroccanoil Moroccanoil Luminous Hairspray / Sinus Rinse Regular Mixture Packets - Relieves Allergies & / Charlie's Soap Powder Bucket, 1000 Loads, 32 Pounds / Jarrow Formulas Quercetin 500mg, 100 Capsules / NOW Foods Liquid Vitamin D-3 5000Iu/Dropper, 2 ounce
Manufacturers seldom provide directly to the public, but they make use of gross sales reps to be in complaint of flaring large shiploads of trade goods to district wholesale suppliers or distributors, who in crook vend to the retailers (us). These gross revenue reps cognize scientifically who their wholesalers are.
So how do you discovery who manufactures the product?
Well, for a embark on you can visage at the product itself. If you are mercantilism DJ equipment, go to a shop and have a exterior at the pay for of it. Sometimes, if you are remarkably auspicious and you countenance on the spinal column of your selected wares it may retributive say 'Distributed by..." Make a make a note of of any records you can insight everywhere on your merchandise and set off near.Post ads:
Estee Lauder 2012 Fall Collection 7 pcs Skin Care and / Mastex Professional Heated Beauty Booties / The Original Prostretch / Lavette Bottle - Perineal Irrigation Bottle Model / Equal Zero Calorie Sweetener -New 800 Packets / Posture Brace by PostureNOW (Small) / NOW Foods Policosanol 20mg Plus, 90 Vcaps / Vanilla Essential Oil. 10 ml. 100% Pure, Undiluted, / Rayovac 6V-HD 6-Volt Heavy Duty Lantern Battery / AmLactin Moisturizing Body Lotion, 14.1-Ounces / Kirkland Signature Extra Strength for Men Minoxidil / Steripod Clip-on Toothbrush Sanitizer (8 Steripods) 4 x 2 / Pyle Sports PHRM38PN Heart Rate Monitor Watch with / Kiehls - Panthenol Protein Moisturizing Face Cream / Keratin Coppola Complex Infusion Keratin Replenisher 1.7 / Raw African Black Soap from Ghana - 1 Lb / Vidal Sassoon VS783 1875-Watt Professional Anti-Static Ion
You after do a turn out for the touchtone phone digit (either online or done the country's light-colored pages) and telephone the company. The secretary will ask you who you would same to shout to. This is where on earth it helps to be a registered concern.
"Hello, I am Jonathon Smith of Smith Retailers Ltd. Could I communicate to a income rep please?"
She transfers you finished to Tim in the gross revenue bureau.
"Hi Tim, My moniker is Jonathon Smith of Smith Retailers Ltd. I was hoping you could tender me your commissioned suppliers database so I can interaction them."
Tim says no worries and will stock you near the wholesale suppliers. This is Tim's job, and he will be more than sunny to impart you next to the wholesalers.
Tim may ask you any questions roughly speaking your conglomerate. Just statement as truthfully as probable or say you are simply researching this attack to discovery out whether or not it is a practicable route.
That's it. You have gone head-on to the point and cut out any middlemen that you will find with an cyberspace furrow.
What's next?
Now you have the information, it is juncture to range out which distributors will trade with you.
So afford the freshman wholesaler a phone call and see what they have to say. You will condition to ask for the income rep again, let's phone call her Susan.
"Hey Susan! My cross is Jonathon Smith from Smith Retailers. I would like to get more than a few figures nearly environment up a retail tale beside you.
Susan will inception to ask you whatever questions active your firm. You will necessitate to persuade Susan that you are competent to unite bottom sales requirements to be able to utilise for a wholesale reason next to her institution. If you truly are momentous give or take a few winning this on, you can hang-up a organization dub and past incorporate the cast.
Be willing for Susan to ask you some other questions roughly speaking your firm that you are newly active to have to reveal the fact for. It really helps to have a business concern create so that you may pasture these questions professionally. A marvellous website do near videos is - this will aid you prophesy your planned sales, confer more or less your viewers & financial backing type, general commercial enterprise overview, analysis, financials etc etc.
If you are new to this, you simply have to be candid as all they have to do is bank check your band information to see you have lone been in business organisation for a small indefinite quantity of weeks. If they insight that you have told fibs, Susan will cut off your hand truthful in the heart of all your auctions.
If Susan won't supply you a wholesale account, go to the subsequent distributer on the account that the first business organisation supplied to you. This is the lone way you can find truthful wholesalers.
This is how middlemen and retailers do it.
Or, you can simply writ through with Salehoo.net.nz - They don't ask any questions and have wholesalers next to up to 95% off retail.
If Salehoo.net.nz doesn't have it, they will find it for you. If within is a wholesaler you poorness substance about, here is a forum of about 35,000 members that you can ask for proposal. That's how I do it, and it couldn't be simpler. It's your verdict.
Happy wholesaling!


text:
1986 Chevrolet El Camino Hot Rod
Price: US $9,999.00 Item location: Auburn, Washington, United States
- Condition: Used
- Make: Chevrolet
- Model: El Camino
- Trim: 2-door
- Year: 1986
- Mileage: 15,000
- VIN: 3gccw80h5gs908236
- Engine size: 383 stroker
- Number of cylinders: 8
- Transmission: Automatic
- Drive type: RWD
- Vehicle Title: Clear Want to buy? Contact seller!
1986 Chevrolet El Camino
Super Clean 1986 Chevrolet El Camino "HELL CAMINO", his monster has 383 stroker, 00r4 transmission, osi rearend, 0" custom rims with matching painted centers, isc brake front and back, ustom stereo, his is a bad ass car and ready to hit the streets! Full air ride suspension, ERY CLEAN set up, his car rides smooth!! Please serious calls only,9,999 OBO.
To see full walk aroundof thecar and to see the car startedclick here:

subset: commoncrawl


text:
Wild Bird Feeders Collection
Our Polywood Duck Box is handcrafted by the Amish in Indiana. Its various convenient features include stainless steel screws, top opening for easy cleaning, post mount, and interior mesh to allow young to crawl up.
Polywood is made from recycled materials, so not only can you feel good about providing a home for ducks, but it will be an environmentally-conscious choice as well! You and your family will delight in the beauty of a family of ducks enjoying their new Polywood home.
Available in a wide variety of colors, you will be able to choose the perfect fit for your outdoor space. Please feel free to give us a call at 866-272-6773 to speak with a friendly DutchCrafters specialist. We'd love to answer your questions.
© Copyrights 2003 - 2017 DutchCrafters All rights reserved.


text:
Current Opening
Senior Graphic Designer Job Opening
Posted: September 8, 2016
Department: Design
The Senior Graphic Designer is responsible for creating extraordinary brand experiences for a variety of high-profile clients and must possess the ability to create compelling communications based on messaging and positioning with an obsession for achieving clarity and emotional connections. The Senior Graphic Designer must have the ability to analyze, plan, and create visual solutions through the application of specialized techniques within a variety of media forms including video, slide presentations, environmental graphics, website design, and interactive multimedia, and must consider visual design essential to the creation of an engaging and intuitive user experience. The Senior Graphic Designer is also responsible for client-facing project presentations and interactions as well as contributing to the team-oriented philosophies established by the Design Director.
- Four-year degree in Graphic Design or closely related degree required
- Four to seven years as a graphic designer within a studio, agency or in-house environment, preferably with strong skills in creative review and quality assessment
- Demonstrate creative ways to present complex information using graphics techniques
- Solid understanding of both business and user goals while having a clear grasp of how to effectively present information in creative and informative ways
- Must exhibit character traits that enable him/her to present concepts and ideas internally and externally with expert authority
- Adaptable to the challenges of a variety of media, as well as adaptable to benefit from creative feedback, internally and externally
- Strong verbal communication skills, presentation skills, and solid writing ability is essential
- Advanced skills in typography, composition, and color
- Must have knowledge, experience, & advanced skills in Adobe Creative Suite and the Mac OS environment
- Must have working knowledge of applications including word processing, spreadsheets, database, & presentation software
- This position may require the capability to learn new applications such as Cinema 4D, Final Cut Pro, Adobe After Effects and more
- Be proactive in understanding all materials associated with the project; its intended use, target audience, and final deliverable. Follow through to the end making sure the project exceeds expectations.
- Work with teammates on all creative graphic components of a project.
- Create logos/type treatments, identity systems, print, web, and screen layouts, attract screens for interactive projects, navigational controls, and other graphic elements as required.
- Maintain consistency and continuity of creative concepts and project details as designed.
- Lay out and produce print, slide, web, environmental/large scale graphics, and other component materials as required.
- Work with outside creative vendors, providing art direction.
- Work with outside production vendors, reviewing and approving proofs and attending press checks as required.
- Write copy that embodies a specific tone or voice.
- Stay abreast of technical and creative resources.
Working Relationships
The Senior Graphic Designer reports directly to the Design Director
So, what do you think?
Do you have what it takes to be an Anodian?
Submit a cover letter and résumé to Anode's Director of Operations via email at firstname.lastname@example.com.
PLEASE NOTE: This position is full-time and requires relocation to Nashville, TN. We will not consider contractors, placement agencies/headhunters, or remote workers.


text: I don't get why people are getting mad at the others pointing out the insensitive wording. This article could have been written much better, and the use of "white guy" and "ring up" are far too casual when speaking of a death. However, "just a few plants" that went towards "funding his education" is complete BS so I, myself, am about to get insensitive. If you can't afford school or need money there are plenty of scholarships, grants, work studies and jobs to be had. I imagine if I had a friend or family member who was shot to death I would be incredibly shocked and saddened, but also extremely aware of how those circumstances came to be. I find it offensive then, to ask students and people to donate to a charity named for him and "Rally for Justice" when hardly anyone remaining at the school knew him and no one is willing to share the truth of the gritty details. I am a CIA student and I had to Google him just to find out what happened. How about rallying for awareness of the perils of getting into drug dealing? Or promoting student job fairs to help students earn the money they need? I have known far too many people who have been robbed on the regular because of even minor drug dealing and I'm fed up with people being so nonchalant about it. It may pay the bills, and you may only sell to friends, but it's dangerous. Friends and family of Brandon should absolutely continue in their search to find his killer, but if I see a single other item dedicated to him and supported by the school I swear to God, I'll start a petition. This wasn't cancer, or a car accident or someone who died attempting to save another person's life, and I don't want to give money to an institution that doesn't see the difference. Something is wrong with society today when we're moping over the loss of celebrities to coke-overdose and stories such as these. Real heroes act honorably even in their secret lives.


text:
Chase Wright: The fact that Wright, who won the Florida State League Pitcher of the Year award in 2006 on the strength of his plus changeup, doesn't crack the Top Ten is a credit to the depth of big-time changeups in the Yankees farm system. It's a pitch he can throw in any count and in any situation and he pounds the lower-half of the strike zone with it.
Christian Garcia: What is perhaps the most frustrating part of Garcia's Tommy John surgery this offseason was he had just developed his changeup into a plus pitch, getting to the point where it was nearly as devastating a strikeout pitch as his hammer curveball. The good news, with his curveball going to be put on the shelf for a good portion of his rehab, the changeup could come back better than ever upon his return.
|Brett Smith will throw his changeup in fastballs counts as often as anybody. (Photo: Patrick Teale/PinstripesPlus.com)
Bryan Villalona: Signed back in 2001, Villalona is often an afterthought by fans because he toiled in the short-season leagues for four years, including the Gulf Coast League for three of them. His rather slow progression through the minor leagues has helped disguise the fact he has one of the better changeups in the organization and it is one of the reasons why, despite the Yankees stockpiling young power arms, he still gets a ton of respect from the Yankees.
Phil Coke: Coke cut his ERA from 2005 by over two full runs in 2006, his best season as a professional by far, thanks in large part to the development of his now plus changeup. He didn't even begin throwing a changeup until his junior year of college and he gets knuckle-ball type of movement on his changeup where it dances around the plate with good command. It's one of the reasons why he's knocking on the door of the Top 50 Prospects.
Top Ten Changeups
10) Dellin Betances: Like Coke, Betances went from a non-existent changeup to a plus pitch, albeit Betances did it in just one short season. With the great movement he's able to generate with it actually makes it one of the better changeups in the organization right now but lacks the overall command of it for it to rank higher on this list. At just 18 years old however, and considering how quickly he has turned his changeup around, there's no telling how far it will develop.
9) Daniel McCutchen: Not only does McCutchen hit the mid-90's with his fastball and throw a great spike-curveball, but he has one of the more advanced changeups in the farm system. His split-changeup gets tremendous sink as it dives down and in to right-handed batters, which is a big reason why Yankees officials believe he could move through the minor leagues quicker than most.
|Zach McAllister was drafted in the 3rd round of the 2006 MLB Draft on the strength of his advanced changeup. (Photo: Patrick Teale/PinstripesPlus.com)
7) Tyler Clippard: The fact that Clippard's 'anytime, any count' plus changeup, a pitch he can consistently throw to location in the 78-81 MPH range, doesn't rank among the top five speaks volumes to the collection of arms in the Yankees farm system. Along with his curveball, it serves as one of his primary strikeout pitches and it's a big reason why he has led his leagues in strikeouts each of the last two seasons.
6) Edwar Ramirez: It might come as a shock to some to see the former Angels' prospect on this list after just a half of a season in the Yankees organization. However, throwing a changeup described by Yankees Pitching Coordinator Nardi Contreras as a "Bugs Bunny-like changeup", the movement Ramirez generates on his change is something you would see in a video game and it's the reason why he posted a 1.17 ERA in 19 appearances for the Tampa Yankees last season. Even with that type of movement, he has incredible command of his plus changeup, really his only main pitch.
|Garrett Patterson has incredible command of a devasting changeup. (Photo: Patrick Teale/PinstripesPlus.com)
4) Garrett Patterson: Known primarily for his blazing fastball from the left side and his long list of injuries that have plagued his career, people often overlook the fact that he has one of the best changeups around. Don't let his high walk totals cloud the fact that he has incredible command of a plus changeup that sits in the 72-74 MPH range. While he does have problems locating his mid-to-upper 90's heat, the 20 MPH difference in his changeup with good command just isn't fair on opposing batters.
3) Ferdin Tejeda: Like Patterson, not many people realize Tejeda has an advanced plus changeup simply because he is a power pitcher who hasn't been on the field long enough to showcase his best offspeed offering. As impressive as his 94-97 MPH fastball and plus slider are, it's Tejeda's plus changeup and the command that he has of it that has many Yankees insiders believing he will be pitching in the big leagues sooner than people realize.
2) Jeff Marquez: Marquez came into the organization out of college already armed with a plus changeup because of the incredible movement he was able to generate with it. And while it has remained one of the best changeups over that time, the fact is he has learned to command it even better in his three years with the Yankees. Until the development of his curveball last season, it served as his primary strikeout pitch and with his changeup giving opposing batters the appearance that it's a breaking ball with the movement he gets, batters might have an even harder time picking it up now that he has a true curveball.
1) Matthew DeSalvo: Like Patterson, DeSalvo has had some command issues in the past but it isn't with his changeup. While he will miss the zone with his fastball at times, DeSalvo can spot his wicked changeup at any time. Even in an arsenal as deep as his, his changeup is his primary strikeout pitch. He not only gets a bunch of swing-and-miss strikeouts with his changeup, but he gets nearly as many called strikeouts and it's the reason why he was averaging a strikeout per inning in his career until last season.


text:
Woman Gave Birth To A Terrifying Snake
Woman Gave Birth To A Terrifying Snake
In Koronadal City, southern Philippines, a married woman [named withheld for privacy
reasons] gave birth to a 2-foot long snake, reports Bombo Radyo Koronadal, a local
radio station.
According to the report posted on radio station's
Facebook page, the 37-year-old woman was
married but her husband is currently in the
country's capital, Manila.
One day, before her husband's scheduled
homecoming on January 5, 2016, the woman
already showed symptoms of pregnancy.
Seemingly with morning sickness, the woman
would frequently vomit and found it diôcult to
eat because of her worsening condition. She also complained of stomachache.
The woman's parents reportedly brought her to 3 diöerent hospitals but not one could
provide them a clue to her condition. When a pregnancy test came back positive, they
scheduled an ultrasound but doctors could not conñrm whether she was carrying a
child since the fetus did not look like a typical baby but had a long body.
Fearful for their daughter's life, the woman's parents brought her to faith healers. They
went to a total of 7 witch doctors but only the last one positively told them he could heal
their daughter.
The woman underwent 'treatment' for a month under the last witch doctor. Then, on
February 16, Tuesday, with the assistance of the witch doctor who encouraged her to
'push' the baby amid birthing contractions, the woman allegedly gave birth to a creature
which looked more like a snake than a baby.
The birth was supposedly witnessed not just by the witch doctor and the woman's
parents but by other relatives and onlookers as well.
Upon seeing the creature which allegedly had teeth that resembled those of a piranha,
the woman's mother pierced it to death using a sharpened 'bagakay' (a thin type of
bamboo believed to Òght/counteract evil and supernatural creatures). Not content with
killing the creature, the woman's mother also burned the snake's body.
According to the report, the woman is now recuperating and the family had requested
for their identities to remain anonymous.


text:
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text:
The Prose and Poetical Works of Heinrich Heine...
New York: Printed for Subscribers only by Croscup and Sterling Company, [1902]. Düsseldorf edition, number 15 of 250... Read More
Price: $2,250
New York: Printed for Subscribers only by Croscup and Sterling Company, [1902]. Düsseldorf edition, number 15 of 250... Read More
Price: $2,250
Washington: [Privately printed at the Press of J. H. Furst Co., Baltimore.], 1910. First edition. Signed by Adams at the end of the introductory letter, as it seems that most copies were.Adams, historian and a direct descendant of John Quincy Adams, published and disseminated widely this present work. It is... Read More
Price: $750
London: Saunders and Otley, Conduit Street, 1843. First Edition in English, with half-titles. Read More
Price: $450
New York: Weltdemokratische Vereinigong, 1939. Read More
Price: $250
Tromøy (Norway): 1 September 1939. 1 September 1939: The Poet in Exile. Poet, pacifist, and journalist Max Barth (1896-1970) worked for the Süddeutschen Sonntagszeitung and was among those who called for a general strike to protest the Nazi putsch. He was convicted of high treason and fled to Switzerland. His... Read More
Price: $850
[New York]: September 17, 1928. Benêt on John Brown: "I tried to do my best with him…". Fascinating letter, written soon after the publication of Benêt's most enduring poem, JOHN BROWN'S BODY (1928), to America's most eminent literary agent of the day, Major Pond. "Thank you very much... Read More
Price: $600
Leipzig, Berlin: Insel-Verlag, etc, 1928-1936. First editions of the first four titles, later editions of the last two titles. He Created the Myth of Nietzsche. Ernst Bertram (1884-1957), Germanist and poet, was appointed professor of German literature and philology at the University of Cologne in 1921. His friends included Stefan... Read More
Price: $3,000
Seal Harbor, Maine: Printed & Sold at High Loft, (1982). Number 122 of copies 51 to 200 of the regular edition. Printed in 16 pt. Blado, on Gutenberg paper at High Loft, the printing office of August Heckscher; by Tom Coleman and Karen MacDonald, printers. Read More
Price: $450
Berlin: Suhrkamp Verlag, 1957-1965 (Volume 9 is dated 1965). All are first editions except Vol I and IX. Read More
Price: $300
[Munich: Bremer Presse, 1932]. First edition, no. 61 of 85 copies, signed by Martin Bodmer on the colophon. One of 85 copies. Scarce trial specimen in preparation for a proposed edition of Goethe. Read More
Price: $1,250
Leipzig: Im Insel-verlag [Boeschel & Trepte], 1920. Number 455 of 520 copies of this the first separate edition of this tragedy. It was from this work that the Austrian composer Alban Berg composed his very successful opera "Wozzeck" (1925). These fragments were first published posthumously in 1850 as "Wozzeck". Read More
Price: $250
Chicago: A.C. McClurg & Co, 1914. First edition, first state, with printer's name set in two lines of Old English type. The first edition of the first Tarzan novel — a brighter than usual copy.The child of Lord and Lady Greystoke who were marooned in West Africa, Tarzan ("White Skin"... Read More
Price: $4,500
New York: Hurd and Houghton, 1871. First edition. First Book. Read More
Price: $250
New York: Random House, (1965). First Trade edition. Read More
Price: $175
New York: The Macmillan Company, 1906. Shinn, Florence. Number 40 of 100 copies of this special edition on Japanese were printed June, 1906, of which sixty are for sale. With TLS laid in mentioning working on this book and his regets that he cannot come to ABA dinner honoring him. Read More
Price: $150
New York: Charles L. Webster, 1885. First American edition, mixed state. The first edition of Twain's enduring classic of boyhood, the book that prompted Hemingway to state: "All modern literature comes from one book by Mark Twain. It's the best book we've had. All American writing comes from that. There... Read More
Price: $1,500
Altdorf, Weingarten, etc. [Germany]: 1852-1862. Charming and decorative mind-nineteenth-century German album of sentiments, most dated variously between 1852 and 1854. On the verso of a poem headed Trennung (Separation) and inscribed "Tobis seiner liebsten Anna" is a portait of a pensive young man and the caption "Jetzt bin ich's nicht... Read More
Price: $750
New York: James G. Gregory (Successor to W.A. Townsend & Co.), 1862. First edition. India Proofs. Read More
Price: $750
London: Henry Colburn, New Burlington Street, 1828. First edition. Read More
Price: $1,500
Philadelphia: Carey, Lea & Blanchard, 1836. First edition. Read More
Price: $250
Dresden: Printed for Walther, 1830. First edition, one of just a few copies to survive, the rarest of Cooper's works. The Dresden Water Witch. The Water Witch was written in Naples and was first printed in Dresden in September 1830, a month before the English edition, and two months before... Read More
Price: $12,000
New York: John Wiley, 1834. First edition. Read More
Price: $750
London: C. Baldwyn & James Robins, 1823 (?)-1826. Cruikshank, George. Later edition. UNBOUND and UNCUT!!! The Woodin Copy. The unbound sheets of an unidentified, early edition with plates on India paper, of one of Cruikshank's early masterworks, whose etchings Ruskin described as "unrivalled in masterfulness of touch since Rembrandt." These... Read More
Price: $2,500
London: G. & W.B. Whitaker, 1824. Cruikshank, George. First edition, third issue, with title dated 1824 (not 1823) and without a hyphen in 'Ave Maria' on title page. Read More
Price: $450
Potsdam: Müller, 1920. Possibly First German Edition. Read More
Price: $175
Boston: Printed at the Riverside Press, [1883-1893]. Riverside Edition. Read More
Price: $500


text:
Princess Cancer Pants
• Chemo
• Chemo-Radiation
• Surgery
• Return to kicking my own ass by 2018
She was not strong. She was valiant. Radiant. Brave and broken. The beauty she discovered in the aftermath was unparalleled to anything she had known before, because it had come at such a cost.
~ Unknown
Roads were made for journeys...
You'll ruin your knees!
""...the truth that someday, you will go for your last run. But not today—today you got to run." - Matt Crownover (after Western States)
Apparently you don't need to run slow in order to make use of the "start early" option, though? Your link shows quite a few people running sub-3:00 with an "E". But perhaps people who are really gunning for a PR would try to do it with fewer runners around to get in the way? It still doesn't make sense, some how...
By the way... check out the 80 year old who ran a 3:20! Go Wally!


text:
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Sv Dewoolkar
Address: Room No 4, Daoachanji Bldg., Nesbit Road, Mazgaon, Mumbai, Maharashtra, 400010
Phone number: 022 - 24930326Post A Recommendation Bookmark this Business Claim this Business
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text:
Standing pat this year
View Single Post
Registered User
Join Date: Jun 2012
Location: Winnipeg, Manitoba
Posts: 663
vCash: 500
Winnipeg NEEDS to really consider moving their UFA's.
The biggest thing this team is lacking which was discussed in another topic was a winning culture. Nik Antropov and Ron Hainsey hold great value but they aren't winners. Winnipeg should focus on the core they have right now and continue to nurture the development of their youth. If anyone thinks this team or this organization of players is a Stanley Cup contender should reconsider that stance. The likelihood of the players we have developing into superstars is minimal. This would open roster spots for 2013-14 for Zach Redmond and Mark Scheifele who would fit into very well into those spots. Bogo/Enstrom/Stuart/Buff can play big minutes on the point while Redmond continues to adjust to the NHL and the risk vs. reward of acquiring prospects and/or draft choices for those two, in my opinion, way outweighs the risk of the players we acquire being busts or Hainsey and/or Antropov taking their game to the next level.
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text: Yes and no, they seem to have organized groups and occupy territory together, but there is no known official governing system or overall rulers such as a King or Queen. Many NPCs often mention that the Super Mutants seem to be looking for something, but as far as I know, nobody ever clarifies what it is they are looking for. A social class also seems to be present with regular Super Mutants, Brutes, Masters, and Behemoths(if you consider them a type of Super Mutant). If you play through the main quest, you will eventually stumble upon clues of how the Super Mutants came to be as well as how they are discriminant to certain members of their kind, like those who have intelligence or a good disposition to humans.


text:
The process of buying a new Mercedes-Benz car or truck can seem overwhelming if you don't know where to begin. Edmunds.com can get you started on the right track with a convenient directory of Mercedes-Benz car dealerships in and around Ava, New York. Compare online price quotes on the new or used car, truck, SUV, minivan, or wagon of your choice to locate the best deals. Edmunds.com makes it easy to find trusted Mercedes-Benz car dealers in Ava, saving car buyers time and money on what will be an important purchase.
Non Display Review Name "Very friendly, helpful, and patient. We got the exact Jeep we wanted. Everyone involved in the transaction was profession and efficient." Read more Review by: DTChittenango1


text:
Dr. Martens 939
Product Information
- The Dr. Martens® 939 6-Eye Boot has classic construction with polished look.
- Smooth leather upper.
- Classic six-eyelet boot built on a comfort last.
- Padded collar for added comfort.
- Classic back pull loop.
- Goodyear® welt sews and heat seals the upper and sole together for better durability and flexibility.
- Leather and textile lining for added comfort and breathability.
- Lightly cushioned footbed for added comfort.
- Air-cushioned outsole is oil and fat resistant and has great abrasion and slip resistance.
- Imported.
- Measurements:
- Heel Height: 1 1⁄4 in
- Weight: 2 lbs 9 oz
- Shaft: 6 in
- Platform Height: 3⁄4 in
- Product measurements were taken using size UK 11 (US Men's 12), width M. Please note that measurements may vary by size.


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British Pubs, South West
Pub Rooms B&B British Pubs, South West
Six boutique letting rooms, prices are per room, per night and include full English breakfast. To book rooms or to enquire about availability please... DT6 3LY
Located just off the main A39 Bridgwater to Minehead road in the hamlet of Bradley Green, just outside the village of Cannington, and two miles from the...
Luxury B&B accommodation available, Extra double bedroom available on request. The pub has been a hostelry, welcoming travellers and visitors ever...
The Nags Head offers accommodation in the form of 4 bedrooms operated on a Bed and Breakfast basis.
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The London Inn at St Neot is a traditional English Pub with Three BB Rooms
Real community pub with Rooms in a boutique hotel style. Bedrooms expectionally high standard. Competitive Room prices range from 69 - 139...
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Eight bedrooms all with en suite facilities, WiFi, telephone and T.V. (one family room which sleeps up to 6 people). Breakfast included
13 accommodation rooms ranging from our comfortable standard rooms in the Coach House to the suites in the main building.
Traditional pub hotel in the heart of Wiltshire with BB accommodation, a convivial atmosphere. The Crown Hotel in Trowbridge
Devon Bed And Breakfast - our 16th Century Country Pub comes equipped with three en-suite guest rooms with double bed or twin options, just a stones...
3 comfortable BB rooms, Family Room with double bed and single bed. Another single bed can also be provided in this room if required.
Five beautiful, en-suite guest rooms breakfasts included. Each room unique.
Situated next door to the new Travelodge in Newquay, the Griffin offers a friendly welcome and the traditional comfort associated with Bed...
15 En Suite Bedrooms at the White Hart Hotel, in Wells Somerset
Bed Breakfast available in our accommodation house next door to the pub.
15th century coaching inn, a stone's throw from Wells Cathedral and the Bishop's Palace, in the heart of England's smallest City.
The Lopes Arms is a 17th Century former Coaching Inn situated in Roborough village, just 7 miles from the city centre of Plymouth and within easy reach...
Traditional free house country pub in the village of Corton, few miles from Warminster,Longleat, Stonehenge and Bath.Accomodation consists of five...


text:
Currently 0.00/512345 Rating: 0.0/5 (Total votes: 0)
Rating: 0.0/5 (Total votes: 0)
PSP CheatUp was designed to simplify the task of updating the cheat codes of any PSP cheat engine through the use of the PSP's Wifi and a wireless internet connection. The cheat files are hosted by GBAtemp.
Feature Summary
* Display the Changelog and Gamelist.
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* Update application from the PSP.
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Change Log (0.41)
* Added:A cheat.db file extraction can be overridden for CMF by holding L trigger when before installing cheats.
* Other:A A Removed 6.xx compatibility due to causing compatibility issues.
Change Log (0.40)
* Added:A A Region filtering (EU, US and JP) for GameList.
* Added: A GameList search function (if search term is a Game ID available cheats are listed).
* Fixed:A A A Problem extracting files from ZIP archive which aren't compressed.
* Other:A A CMF and NitePR cheat files are only extracted if the file size is different to the file in the archive.
* Other:A A Will now load on 6.xx custom firmwares.


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Folio (2 items)
Welcome back! Whilst you were away we added the functionality to view auctions and bid in pounds.
Folio: Information on the Catawiki site about Folio, 2001.
At its heart, Catawiki is an online catalogue with images of, and information on, collectibles. Our catalogue is compiled by users in a similar way to Wikipedia.


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Silver price is now just below $30 per troy ounce. Silver priced peaked in 2010 and reported a great year. January's performance was lacking overall, but this is still a great time to buy silver bullion. Silver's stability in the market, despite being a volatile metal that moves with the market, is proof of the metal's high demand. If you're still nervous about buying, consider four reasons to start buying this precious metal before the first quarter ends.
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Silver is the alternative to gold and it benefits from investment complications of gold—such as hoarding, rising prices and supply issues. Silver has less value than gold and thus can be more easily converted into cash. Gold on the other hand, is so valuable it's an investment tool and is usually kept in storage or in a bank.
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Lazise Travel GuideBrowse 3 travel reviews, travel blogs and 46 travel photos from real travelers to Lazise.
Check Lazise Hotel Deals
Lazise Overview
Welcome to the travel guide for Lazise. Lazise travel reviews and blogs are contributed by millions of fellow travelers, providing you with an unbiased perspective of Lazise.
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Perhaps one of the most famous destinations in north-east Italy, well-known for sights such as the Roman Arena dating back to the 1st century AD, the Roman amphitheater looking over the city …
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Why Jesus?
Why Jesus?
Rediscovering His Truth in an Age of Mass Marketed Spirituality
Faithwords, Hardcover, 9780892963195, 304pp.
Publication Date: January 25, 2012
Major new age and human potential tenets will be discussed like: the belief that we are all gods and have to discover our divinity; Jesus was only a good teacher; Christianity is but one among many ways to eternal life; reincarnation is real; Jesus was married; truth is relative; there is no sin; and perfection is possible.
The truth of and the arguments for the bodily resurrection of Jesus will be presented as the most important argument for the exclusive claims about Jesus and Christianity.
"Ravi Zacharias gives a powerful defence of how Jesus Christ brings meaning and hope to an individual life."
"Ravi takes a scholarly yet imaginative approach to aplogetics."
-Bruce Wilkinson, author of the #1 "New York Times" bestseller The Prayer of Jabez
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"To every generation God sends a prophet. Ravi Zacharias is that prophet for this generation. You must hear him."
"Ravi Zacharias presents an outstanding defence of the Christian faith for our times."


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Accommodations in FangchenggangSee All
Discover the beauty of the city's striking landscope, welcome to Fangchenggang, China. This city is located in the close proximity of a major city Nanning, China. Take a short trip from Fangchenggang and set some time aside to explore the Nanning. Dotted with places like Qingxiu Mountain Scenic Spot, Detian Waterfalls, Nanning Zoo; it will definitely be a good idea. Get in the character of the city, this city requires at least of 1 to 2 number of days of exploration.
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text: Raul Zelaya of Death To Sour Mix Latest Stories Sponsored Summer Crush: Three Refreshing Cocktails You Can Whip Up Now May 5, 2015 Share This Contributor Share on Facebook. Tweet it! Pin It! Email to a Friend Share on Google Plus. Share on LinkedIn. Print this Page Sign Up for the Daily Traveler Enter your email address Sign Up Please enter a valid email. We encountered a server error. Please try again later. Thank you! A welcome email has been sent.


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Went to the annual Pultneyville Antiques Fair yesterday. Scorcher. Even though it was an afternoon for icy cold watermelon, against all good sense and our vow to avoid sweet crap, we bought and devoured a funnel cake, spilling confectioner's sugar all over our guilty selves. We then walked around in a sugar daze, wondering where we parked the car.
Wicked fun.


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- Worldwide Rank #25,368,860
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@00007419 - Tracking Riyadh Halawani Twitter profile since November 18, 2011
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Black Bean Soup
Recipe by LMillerRN
A fiesta of flavors serve with tortilla chips or corn bread for a filling meal.
Top Review by missystry
Easily a 5 star either as written or with my following additions: I added about 1 cup frozen corn, 1 can mild green chili peppers, and, added about half again the amount of onion and garlic. When serving, I top with a sharp cheddar cheese, sour cream, thinly sliced green onions or minced yellow onion, crushed tortilla chips, a generous squeeze of fresh lime juice and fresh cilantro roughly chopped. (In that order.) We like to use buttered corn tortillas for scooping up the juices. It should be noted that this soup is every bit as good....if not better....the next day when the flavors have had a chance to meld. Of course only top when ready to serve.
See Discussion:18 Reviews
Ingredients Nutrition
- 2 (16 ounce) cans black beans, undrained, divided
- 1 cup reduced-sodium chicken broth
- nonstick cooking spray
- 1 small onion, chopped
- 1 teaspoon bottled minced garlic
- 1 (16 ounce) jar chunky salsa
- 4 teaspoons lime juice
- 2 teaspoons ground cumin
- 1⁄4 teaspoon crushed red pepper flakes (optional)
- 1⁄3 cup plain yogurt (optional)
- fresh cilantro leaves
Directions See How It's Made
- PLACE 1 can of beans with liquid and broth in blender or food processor; cover. Blend until smooth. Coat large saucepan with cooking spray. Heat over medium-high heat. Add onion and garlic; cook for 4 to 5 minutes or until onion is tender.
- ADD blended bean mixture, remaining beans and liquid, salsa, lime juice, cumin and crushed red pepper. Bring to a boil. Reduce heat to low; cover. Cook, stirring occasionally, for 25 to 30 minutes. Serve topped with yogurt. Garnish with cilantro.


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Durbar Hall, in the Nazarbag Palace [Vadodara]
Medium: Photographic print
Date: 1895
Photograph of the durbar hall in the Nazar Bagh Palace at Baroda, Gujarat from the Curzon Collection, taken by an unknown photographer during the 1890s. The palace was originally built in 1721 and was subsequently altered in the late 19th century by the Gaekwar Maharaja Malhar Rao (ruled 1870-75). It is a white stucco three-storey building designed in the Corinthian style and set in pleasure gardens. The interior is decorated and furnished in a grand western style, as shown in this view of the durbar or royal audience hall containing upholstered furniture and ornate Venetian chandeliers.


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Palden Lhamo (pronounced: 'Pelden Lhamo')[1], Palden Lamo (Wylie transliteration: dpal ldan lha mo), Shri Devi (Sanskrit), or Ukin Tengri (Mongolian) is a protecting Dharmapala of the teachings of Gautama Buddha in the Gelug school of Tibetan Buddhism. She is also called Remati.[2] She is the wrathful deity considered to be the principal Protectress of Tibet.[3]
Palden Lhamo is the consort of Mahakala and has been described as "the tutelary deity of Tibet and its government",[4] and as "celebrated all over Tibet and Mongolia, and the potent protector of the Dalai and Panchen Lamas and Lhasa."[5]
Palden Lhamo and the lake Lhamo La-tso
It is said that Palden Lhamo, as the female guardian spirit of the sacred lake, Lhamo La-tso, promised Gendun Drup, the 1st Dalai Lama in one of his visions "that she would protect the reincarnation lineage of the Dalai Lamas." Ever since the time of Gendun Gyatso, the 2nd Dalai Lama, who formalised the system, the Regents and other monks have gone to the lake to seek guidance on choosing the next reincarnation through visions while meditating there.[6]
The particular form of Palden Lhamo at the lake Lhamo La-tso is Gyelmo Maksorma, "The Victorious One who Turns Back Enemies". The lake is sometimes referred to as "Pelden Lhamo Kalideva", which indicates that Palden Lhamo is an emanation of the goddess Kali, the shakti of the Hindu god Siva.[2] Gyelmo Maksorma (rGyal mo dMag zor ma) or Machik Pelha Shiwai Nyamchen (Ma gcig dPal lh Zhi ba'i nyams can) is an unusually peaceful form of Palden Lhamo.[7]
The mountain to the south of Chokorgyel Monastery, Shridevi, is the "blue" residence of the Protectress Palden Lhamo on which a dundro or sky burial site is located.[8] The monastery was originally built in a triangular form to reflect the symbolism of its position at the confluence of three rivers and surrounded by three mountains and also represents the conjunction of the three elements of water, earth and fire, as well as the female principle of Palden Lhamo in the form of an inverted triangle.[9]
Traditional accounts
It is said that, during the reign of Songtsen Gampo (605 or 617? – 649), Palden Lhamo outdid all the other protector-deities in her promise to protect the king's Trulang shrine. She presented an iron cup and pledged "Erect an image of me, and I shall protect this royal shrine from any future damage by humans and mamo demons!' She is also said to have advised Lhalung Pelgyi Dorje to kill the anti-Buddhist king Langdarma in 841 CE, and is described as the 'Dharma-protectress of Lhasa'.[10]
Part of a series on Tibetan Buddhism
|Timeline · Related-topics
|Nyingma · Kagyu · Sakya · Gelug · Bön
|Key Concepts
|Three marks of existence · Skandha · Cosmology · Saṃsāra · Rebirth · Bodhisattva · Dharma · Dependent Origination · Karma
|Major Figures
|Gautama Buddha · Padmasambhava · Je Tsongkhapa · Dalai Lama · Panchen Lama · Lama · Karmapa Lama · Rinpoche · Geshe · Terton · Tulku
|Buddhahood · Avalokiteśvara · Four Stages of Enlightenment · Tantric yoga · Paramitas · Meditation · Laity
|Changzhug · Drepung · Dzogchen · Ganden · Jokhang · Kumbum · Labrang · Mindroling · Namgyal · Narthang · Nechung · Pabonka · Palcho · Ralung · Ramoche · Sakya · Sanga · Sera · Shalu · Tashilhunpo · Tsurphu · Yerpa
|Chotrul Duchen · Dajyur · Losar · Monlam · Sho Dun
|Kangyur · Tengyur · Tibetan Canon · Mahayana Sutras
|Sand mandala · Thangka · Ashtamangala · Tree of physiology
Outline · Comparative Studies · Culture · List of topics · Portal
She is the only female among the traditional 'Eight Guardians of the Law' and is usually depicted as deep blue in colour and with red hair to symbolise her wrathful nature, crossing a sea of blood riding side-saddle on a white mule. The mule has an eye on its left rump where her angry husband's arrow hit it after she killed her son and used his skin as a saddle blanket, before she became converted to a protector of the dharma. She is considered to be a form of the Hindu goddess Mahakali and also a wrathful emanation of Saraswati.[11] She has three eyes and is often shown drinking blood from a human skull. Moreover, H. C. Beasley claims: "Palden Lhamo was believed by the Tibetans to have been reincarnated in the late Queen Victoria, and as one of the Eight Terribles, arrayed in her full regalia, she lacks nothing of the horrible, and, outwardly at least, lives up to her reputation."[12]
- ↑ Tucci, Giuseppe (1970). The Religions of Tibet. Translated from the German and Italian by Geoffrey Samuel, p. 302. University of California Press, Berkeley and Los Angeles. ISBN 0-520-03856-8 (cloth); ISBN 0-520-06348-1 (pbk).
- ↑ 2.0 2.1 Dowman, Keith. (1988). The Power-Places of Central Tibet: The Pilgrim's Guide, p. 260. Routledge & Kegan Paul Ltd., London. ISBN 0-7102-1370-0 (pbk).
- ↑ Dowman, Keith. (1988). The Power-Places of Central Tibet: The Pilgrim's Guide, pp. 255, 259. Routledge & Kegan Paul Ltd., London. ISBN 0-7102-1370-0 (pbk).
- ↑ "The Boneless Tongue: Alternative Voices from Bhutan in the Context of Lamaist Societies". Michael Aris. Past and Present, No. 115 (May, 1987), p. 141.
- ↑ Schram, Louis M. J. (1957). "The Mongours of the Kannsu-Tibetan Border: Part II. Their Religious Life." Transactions of the American Philosophical Society. New Series, Vol. 47, No. 1, (1957), p. 21.
- ↑ Laird, Thomas (2006). The Story of Tibet: Conversations with the Dalai Lama, pp. 139, 264–5. Grove Press, N.Y. ISBN 978-0-8021-827-1.
- ↑ Dowman, Keith. (1988). The Power-Places of Central Tibet: The Pilgrim's Guide, pp. 78, 260, 344. Routledge & Kegan Paul Ltd., London. ISBN 0-7102-1370-0 (pbk).
- ↑ Dowman, Keith. (1988). The Power-Places of Central Tibet: The Pilgrim's Guide, p. 258. Routledge & Kegan Paul Ltd., London. ISBN 0-7102-1370-0 (pbk).
- ↑ Dowman, Keith. (1988). The Power-Places of Central Tibet: The Pilgrim's Guide, p. 257. Routledge & Kegan Paul Ltd., London. ISBN 0-7102-1370-0 (pbk).
- ↑ Clear Mirror on Royal Genealogy by Sakyapa Sonam Gyaltsen (1312-1375), translated by McComas Taylor and Lama Choedak Yuthok as: The Clear Mirror: A traditional account of Tibet's Golden Age, pp. 173, 265. Snow Lion Publications, Ithaca, New York. ISBN 1-55939-048-4.
- ↑ Palden Lhamo
- ↑ Beasley, H. C. (1927). "A Crystal Mask from Tibet", p. 1. MAN: A Monthly Record of Anthropological Science. Vol. 27 (Jan., 1927). Royal Anthropological Institute of Great Britain and Ireland.


text:
Surveillance technology is developing with breath-taking speed. This creates new instruments in the struggle against terrorism and organised crime, but also raises fundamental questions on the right to privacy for everyone. Individuals should be protected from intrusions into their private life and from the improper collecting, storing, sharing and use of data about them. Terrorism and organised crime must be combated — but not with means which undermine basic human rights.
Monday, May 26, 2008
Privacy and terrorism
The Council of Europe's Commissioner for Human Rights has published a new article on privacy and the fight against terrorism:
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text: DFC Global Corp. (NASDAQ: DLLR) will host a conference call on April 30, 2012 at 5:00 p.m. ET to discuss the Company's fiscal 2012 third quarter results. Investors can participate in the conference call by dialing (888) 200-2794 (U.S. and Canada) or (973) 935-8766 (International); use the confirmation code "Dollar." Hosting the call will be Jeffrey A. Weiss, Chairman and CEO, and Randy Underwood, Executive Vice President and CFO. For your convenience, the conference call can be replayed in its entirety beginning from two hours after the end of the call through May 14, 2012. If you wish to listen to the replay of this conference call, please dial (855) 859-2056 (U.S. and Canada) or (404) 537-3406 (International) and enter passcode "70470299". The conference call will also be broadcast live through a link on the Investor Relations page on the DFC Global Corp. Web site at www.dfcglobalcorp.com. Please go to the Web site at least 15 minutes prior to the call to register, download and install any necessary audio software. About DFC Global Corp. DFC Global Corp. is a leading international diversified financial services company serving primarily unbanked and under-banked consumers who, for reasons of convenience and accessibility, purchase some or all of their financial services from the Company rather than from banks and other financial institutions. Through its over 1,300 retail storefront locations, Internet websites and mobile phone and other remote platforms, the Company provides a variety of consumer financial products and services in eight countries across North America and Europe—Canada, the United Kingdom, the United States, Sweden, Finland, Poland, Spain and the Republic of Ireland. The Company's products and services, principally its short-term single-payment consumer loans, secured pawn loans, check cashing services and gold buying services, provide customers with immediate access to cash for living expenses or other needs. The Company strives to offer its customers additional high-value ancillary services, including Western Union® money order and money transfer products, foreign currency exchange, reloadable VISA® and MasterCard® prepaid debit cards and electronic tax filing. In addition to its core retail products, the Company also provides fee-based services in the United States to enlisted military personnel applying for loans to purchase new and used vehicles that are funded and serviced under an exclusive agreement with a major third-party national bank through the Company's branded Military Installment Loan and Education Services, or MILES®, program.


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Scotland Freedom of information
- Freedom of Information Act (Scotland) (2009) - Which? - Consultation response (PDF: 86Kb)
14 January 2009
Responded to Government plans to consider extending the Scottish Freedom of Information Act and obligations to additional organisations, advocating the Government concentrates on the original purposes of the Act and the rights of individuals.
- Freedom of Information Act (Scotland) (2006) - Which? - Consultation response (PDF: 50Kb)
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Haunt Available on Kinect Arcade for Free
June 26, 2012 - Microsoft is celebrating a Kinect-centric hub on Xbox Live with a free game you might actually want to play.
This Week on Xbox Live (01.18.12)
January 18, 2012 - The middle of the week doesn't only signal the approach of the weekend, it means new content on Xbox Live. Check out the latest.
Kinect in 2011: What's Coming Out?
June 17, 2011 - Hey, you have a Kinect! Neat. So what the hell is coming out for it? Check out our list of games slated for this year that will keep you waving your arms like you just don't care.
Game Scoop! Podcast: Tokyo Game Show 2010
September 17, 2010 - Welcome back to Game Scoop!, your weekly dose of gaming news and massive LOLZ. Couldn't make it to Tokyo this year? We've got you covered with all the scoops on Japan's biggest games.
TGS: Microsoft Debuts Hardcore Kinect Games
September 15, 2010 - Microsoft set aside the bulk of its presentation at the Tokyo Game Show to announce several new Kinect titles. Four brand-new titles were shown, all slated for release in 2011.
TGS: Microsoft Keynote Live Blog
September 15, 2010 - Microsoft is set to take the stage and deliver a keynote address to kick off this year's Tokyo Game Show. With game announcements having already been hot and heavy, what will Microsoft do to keep the momentum going? Join us to find out!


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Please remember to follow the manual of style and code of conduct at all times.
Check BNN and Bulbanews for up-to-date Pokémon news and discuss it on the forums or in the Bulbagarden Discord server.
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Inakano Town
From Bulbapedia, the community-driven Pokémon encyclopedia.
Revision as of 16:42, 23 March 2013 by Pokemaster97 (talk | contribs)
| This article is incomplete.
Please feel free to edit this article to add missing information and complete it.
Reason: Dub name.
| Inakano Town
イナカノタウン Inakano Town
|Lost at the League!
(Japanese: イナカノタウン Inakano Town) is an anime-exclusive location in Unova.
It is the hometown of Russet. In Lost at the League!, Russet's friends and family arrived in Vertress City from Inakano Town to celebrate his progression through the early rounds of the Vertress Conference.
|イナカノタウン Inakano Town
|Literally translates to "rural town".
|This article is part of both Project Anime and Project Locations, Bulbapedia projects that, together, aim to write comprehensive articles on the Pokémon Anime and Locations, respectively.


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|| Gori Tere Pyaar Mein - Shake Your Tooh (DJJITENUK) || IDR ||
Gori Tere Pyaar Mein - Shake Your Tooh (DJJITENUK Mashup)
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Correction Appended
Merkin Concert Hall
Joel Sachs's group Continuum, which is kind of a postdoctoral extension of his New Juilliard Ensemble, on Saturday presented a lively portrait of Roberto Sierra, who, now in his mid-40's, is poised for a career jump. Talking briefly to Mr. Sachs during the course of the concert, he mentioned that he was completing a composition for the Philadelphia Orchestra and would then be going on to one for the National Symphony.
This seems a good point for him to be so poised. The six chamber pieces on Continuum's program all showed features that are both characteristic of Mr. Sierra and useful: harmonic textures that are open and bright without being traditionally tonal; bouncy rhythms, often crossing over one another, that come dancing out of the composer's native Puerto Rico; deft instrumentation; and a gift for distinctive gestures that can twist and turn to create streamlined forms. But the two most recent pieces, both written last year, had a new fineness.
In ''Tema y Variaciones,'' for clarinet and piano, the Caribbean syncopations and complex meters are still there, but now as high-pep engines of musical form and wit rather than conveyers of local color. The subject is a harmony, a set of notes that the clarinet keeps reinterpreting, or declining to reinterpret, in a duo whose spiritedness and humor were well caught by Benjamin Fingland and, at the piano, Mr. Sachs.
The piece finds Mr. Sierra coming back close to the Piano Etudes of his teacher, Gyorgy Ligeti, not such a bad place to be. Since the dynamic rhythmic life of Mr. Ligeti's later music was partly prompted by Mr. Sierra, this is his territory as much as it is his teacher's.Continue reading the main story
''Cancionero Sefardi,'' the other new piece on the program, is a beautiful set of Sephardic folk songs with accompaniments that use the hackneyed ''Pierrot Lunaire'' quintet in new and always clear-sounding ways to support the voice and spray sounds above it. Reminiscent of Luciano Berio's ''Folk Songs'' in its sensuality and perky instrumental detail, the piece was gorgeously sung by Wonjung Kim, who was at once touching and voluptuous, and finely played by the ensemble.
Also on the program were two pieces written for Continuum in 1987 -- ''Glosa a la Sombre,'' to an obscure and shapeless text, and ''Descarga,'' a miniature piano concerto with a thumping close -- and two exuberant instrumental trios. PAUL GRIFFITHS
Correction: April 13, 2000, Thursday A brief music review in In Performance yesterday omitted a leader of the chamber group Continuum, which performed works by Roberto Sierra at Merkin Concert Hall on Saturday. She is Cheryl Seltzer, who is, with Joel Sachs, the group's co-leader.Continue reading the main story


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Studying synaptic efficiency by post-hoc immunolabelling
Cellular and molecular mechanisms
In terms of vesicular recycling, synaptic efficiency is a key determinant of the fidelity of synaptic transmission. The ability of a presynaptic terminal to reuse its vesicular content is thought to be a signature of synaptic maturity and this process depends on the activity of several proteins that govern exo/endocytosis. Upon stimulation, individual terminals in networks of cultured cerebellar granule neurons exhibit heterogeneous exocytic responses, which reflect the distinct states of maturity and plasticity intrinsic to individual synaptic terminals. This dynamic scenario serves as the substrate for processes such as scaling, plasticity and synaptic weight redistribution. Presynaptic strength has been associated with the activity of several types of proteins, including the scaffolding proteins that form the active zone cytomatrix and the proteins involved in presynaptic exocytosis.
We have combined fluorescence imaging techniques using the styryl dye FM1-43 in primary cultures of cerebellar granule cells with subsequent
In this study we describe a technique that specifically correlates presynaptic activity with the levels of presynaptic markers. This method involves the use of the styryl dye FM1-43 to estimate the release capacity of a synaptic terminal, and the subsequent
Our findings support those of previous studies and point out to RIM1α as a crucial factor in determining synaptic efficiency. These results also demonstrate that this technique is a useful tool to analyse the molecular differences underlying the heterogeneous responses exhibited by neuronal networks.
Presynaptic active zones (AZ) are specialized axonal sites of fusion that mediate neurotransmitter release into chemical synapses. A complex network of proteins is assembled at axonal sites that generate the so-called cytomatrix at the active zone (CAZ). These proteins interact with other proteins located either at the presynaptic plasma membrane or at vesicular membranes that regulate Ca2+-dependent fusion of synaptic vesicles. The different stages of the synaptic vesicular cycle (docking, priming, exocytosis and compensatory endocytosis) are orchestrated by distinct subsets of proteins, and the amount and interaction of these different proteins are thought to be crucial in determining presynaptic strength. The capacity of a given synapse to efficiently reuse synaptic vesicles has been proposed as a hallmark of maturation, and differences in vesicular reuse appear to underlie the enormous variability of responses observed in cultured neuronal networks
In the present study, we focused on RIM1α as this protein is a key organizer of the active zone and it interacts directly or indirectly with all other known active zone proteins, including Rab3A and Munc13
FM1-43 live cell imaging
To assess presynaptic activity we used cultures of cerebellar granule neurons that are largely populated by glutamatergic neurons
Figure 1
Graphical abstract of FM1-43 experiments and subsequent post-hoc immunolabelling
Graphical abstract of FM1-43 experiments and subsequent post-hoc immunolabelling. Each number represents a different step in the protocol detailed throughout the paper. Briefly, 7DIV cultured cerebellar granule cells (1) were used to assess synaptic efficiency by means of FM1-43 experiments (2). Then, experiment analysis (3) was performed using the routine described in
Additional file 1: Figure S1
Serial reconstruction of the imaged field. A) Synaptic boutons loaded with FM1-43 dye before (to) and after (tend) stimulation with potassium chloride; contrast phase images of the same experiment field (white box) at different magnifications: 60× (B), 40 × (C) and 20× (D). E) Serial reconstruction of 20× cell phase images of the field monitorized during the experiment (white box) and surrounding areas. F) Contrast phase image al 20× magnification showing the field where the experiment was performed after fixing and labelling with the different antibody (matching field). Note the presence of distinctive hallmarks.
Click here for file
The following antibodies were used in the present study: a mouse monoclonal anti-Munc13-1, (1:1000; ref. 126 111 Synaptic Systems); a rabbit polyclonal anti-RIM1 (1:400; ref. 140 003 Synaptic Systems); and a guinea pig polyclonal anti-CB1R (1:300; ref. CB1-GP-Af530, Frontier Institute Co., Ltd.), the latter used exclusively as a presynaptic marker to obtain a positive linear relationship between the channels. To check for the specificity of the antibodies we performed western blot experiments as previously described
Additional file 2: Figure S2
Western blot of RIM1α and Munc13-1 confirmed specificity of both antibodies. A) Western Blot of Munc13-1 (top green), β-Tubulin (bottom, red) and synaptophysin (bottom, green), showing a single band for Munc13-1 with the expected molecular weight. B) Western blot of RIM1α (green, top) and β-Tubulin (green, bottom) showing that both antibodies recognize a single band with the expected molecular weight.
Click here for file
Additional file 3
Supplementary methods.
Click here for file
Labelling procedures
To study the potential relationship between vesicular release efficiency and the levels of presynaptic proteins involved in neurotransmitter release, we selected two proteins with well-established roles in neurotransmitter release: Munc13-1
Figure 2
Alignment of the different channels used for
Alignment of the different channels used for
1 Fixation and blocking: After the removal of the coverslip, rinse it thoroughly in PBS at 37°C and fix by immersion in 4% paraformaldehyde for 15 minutes. Wash twice in PBS and permeabilize the cells for 6 minutes in PBS-0.2% Triton X-100 if immunodetection of intracellular epitopes is required. Wash for 5 minutes in PBS and using a P100 pipette, gently apply 90–100 μl of blocking solution (PBS + 0.05% Triton ×100, 5% goat serum, 5% donkey serum) onto the coverslip and incubate for 1 h at 37°C. It is helpful to leave the coverslips on a Parafilm-covered surface during the blocking and subsequent incubations to avoid spillage of the solution from the coverslip; 2 Labelling with primary and secondary antibodies: Incubate o/n at 4°C with the desired primary antibodies diluted in PBS containing 0.05% Triton X-100 and 2.5% donkey serum. Wash 3 times in PBS containing 0.1% Triton X-100 and twice in PBS alone. Subsequently, incubate the cells with the specific Alexa-conjugated secondary antibodies for 1 h at 37°C. Wash 5 times in PBS and rinse the covers in MilliQ water to remove excess salts.
FM1-43 experiment and
To carry out a semi-automated and unbiased assay of synaptic efficiency on populations of thousands of individual nerve terminals and to specifically correlate each individual response with a given IR value, we employed a routine previously described by Bergsman et al. 2006
Figure 3
RIM1α levels correlate with synaptic efficiency
RIM1α levels correlate with synaptic efficiency. A)
Additional file 4: Figure S3
FM1-43 experiment analysis routine using IgorPro software and ImageJ. A) Parameters used in IgorPro interface to automatically analyze the FM1-43 experiment. This software renders the mask and a set of background subtracted images. B) Import of the QualitySegment.ibw file in ImageJ software to draw a binary mask. The size of the image has to fit with the original images acquired during the experiment. C) Generation of the ROIset with ImageJ software by analyzing particles present in the mask. D) Import of the set of background-subtracted images for further analysis of the experiment with OriginPro.
Click here for file
Additional file 5: Figure S4
Alignment of FM1-43 with IR puncta after
Click here for file
Results and discussion
The intensity of RIM1α, unlike that of Munc13-1, is a
Although for decades primary neuronal cultures have been used to study various aspects of synaptic physiology, including pre- and postsynaptic function
Our results demonstrate that synaptic levels of RIM1α are positively correlated with FM1-43 unloading, which is a direct measure of vesicular reuse and release (Figure
Another important protein involved in the formation of exocytic complexes is the mammalian homologue of UNC13, the multidomain Munc13-1 protein, although we were unable to detect a positive correlation between Munc13-1 levels and synaptic efficiency (Figure
Figure 4
Munc13-1 levels do not correlate with synaptic efficiency
Munc13-1 levels do not correlate with synaptic efficiency. A)
In the present study, we present a method that can be used to correlate a functional parameter of synaptic physiology (SV release) with the levels of different proteins present at a given release site. The main advantage of this technique is the reduced user intervention during data processing, as manual selection and drawing of the different ROIs is avoided. In most studies assessing synaptic function using image techniques, ROIs are user-defined and as such, they represent a source of potential bias. This bias is not only due to the poor sensitivity of the human eye compared with processing software but also, because the ROI shape does not exactly match that of the synaptic boutons, resulting in the inclusion of dead pixels in the ROIs defined, which can in turn affect the numerical data obtained. Moreover, manual selection usually renders fewer ROIs, which is not optimal for high-level statistical tests. In our protocol we employed an automated routine
Altogether, our results indicate that nerve terminal content of RIM1α strongly correlates with the release capacity of the nerve terminal measured with FM1-43, while no such a correlation was found with Munc13-1. This finding point out to RIM1α as a crucial factor in determining synaptic efficiency and demonstrate the usefulness of this technique to analyse the molecular differences underlying the heterogeneous responses exhibited by neuronal networks.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
JRF, JSP and MT conceived the study. JRF, BA and DBM designed experiments, and were responsible for collect, analyse and interpret the data; JRF and JSP wrote the manuscript. MT and JSP acquired funding necessary for the completion of the study. All authors read and approved the final manuscript.
We thank María del Carmen Zamora for her excellent technical assistance. This work was financed by grants from the Spanish MINECO (BFU2010-16947 to JS-P and BFU2012-32105 to MT), the 'Instituto de Salud Carlos III' RD06/0026 and the 'Comunidad de Madrid' (CAM-I2M2 2011-BMD-2349 to J S-P and MT). We thank Dr. M Sefton for editorial assistance.
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages
Dynein light chain regulates axonal trafficking and synaptic levels of bassoon
Rab3 dynamically controls protein composition at active zones
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing
Homeostatic synaptic plasticity: local and global mechanisms for stabilizing neuronal function
Calcium-independent inhibitory G-protein signaling induces persistent presynaptic muting of hippocampal synapses
Release probability of hippocampal glutamatergic terminals scales with the size of the active zone
A Munc13/RIM/Rab3 tripartite complex: from priming to plasticity?
A post-docking role for active zone protein Rim
RIM1alpha is required for presynaptic long-term potentiation
RIM1alpha forms a protein scaffold for regulating neurotransmitter release at the active zone
Multiple roles for the active zone protein RIM1alpha in late stages of neurotransmitter release
RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction
RIM proteins activate vesicle priming by reversing autoinhibitory homodimerization of Munc13
Munc13-1 is essential for fusion competence of glutamatergic synaptic vesicles
Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs
Differential control of vesicle priming and short-term plasticity by Munc13 isoforms
GABAergic synapses are formed without the involvement of dendritic protrusions
Inhibitory synapse dynamics: coordinated presynaptic and postsynaptic mobility and the major contribution of recycled vesicles to New synapse formation
Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
NMDA induces post-transcriptional regulation of alpha2-guanylyl-cyclase-subunit expression in cerebellar granule cells
Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles
Cell culture of primary cerebellar granule cells
Quantitative analysis of synaptic vesicle pool replenishment in cultured cerebellar granule neurons using FM dyes
Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release
RIM proteins and their role in synapse function
Reciprocal regulation of presynaptic and postsynaptic proteins in bipolar spiral ganglion neurons by neurotrophins
Membrane depolarization regulates AMPA receptor subunit expression in cerebellar granule cells in culture
RIM promotes calcium channel accumulation at active zones of the drosophila neuromuscular junction
PNAS plus: in vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation
Biomedical image-processing
Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses
CDK5 Serves as a major control point in neurotransmitter release
An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy
A vesicle superpool spans multiple presynaptic terminals in hippocampal neurons
In vivo imaging of intersynaptic vesicle exchange using VGLUT1Venus knock-in mice
Synuclein inhibits intersynaptic vesicle mobility and maintains recycling-pool homeostasis
SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release
The presynaptic active zone
Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion
RIM determines Ca2+ channel density and vesicle docking at the presynaptic active zone
Phosphorylation of RIM1alpha by PKA triggers presynaptic long-term potentiation at cerebellar parallel fiber synapses
Calmodulin and Munc13 form a Ca2+ sensor/effector complex that controls short-term synaptic plasticity
Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis
Move over protein kinase C, you've got company: alternative cellular effectors of diacylglycerol and phorbol esters
Automated criteria-based selection and analysis of fluorescent synaptic puncta
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission
STED microscopy with continuous wave beams
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