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I visited with a friend and slept in a double room. The riad is typical moroccon style. The room was big enough, clean and overall nice. Staff was really friendly and helpful. We were welcomed with hot mint tea and lots of information and advice about the nearby area. Breakfast is served on the "rooftop" with a nice view on the surroundings. Would visit again.
- Official Description (provided by the hotel):
- A few steps from the famous Jemaa El Fna and two minutes of both Bahia and Badii Palace, a former hotel formerly named the Workers (1939) has been restored by the appeal to knowledge internationally known craftsmen of Marrakech.More than a typical hotel, Riad NESMA opens its doors on an exceptional place with its beautiful patio and 7 rooms that have been planned and studied with great care to offer quality services. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Hotels.com, Booking.com, Orbitz, Hotwire, Travelocity and TripOnline SA so you can book your Riad Nesma reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Riad Nesma Hotel Marrakech


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Media Options
Known for his work chronicling the anti-apartheid struggle in South Africa since the 1960s, Danny Schechter has died after a battle with pancreatic cancer at the age of 72.
Schechter got his start as "The News Dissector" in 1970 at WBCN-FM in Boston. He went on to become a prolific television producer who won two Emmys. He was executive editor of MediaChannel.org, co-founder and executive producer of Globalvision production company, and the author of twelve books, including The More You Watch, The Less You Know.
Schechter made six non-fiction films about Nelson Mandela, including "Mandela in America." In January he had just published, When South Africa Called, We Answered: How the Media and International Solidarity Helped Topple Apartheid. In 1988 Schechter created and executive-produced the weekly news program "South Africa Now" that aired on 75 public and cable TV stations. He also co-produced "Rights & Wrongs: Human Rights Television."
Schechter was a frequent guest on Democracy Now! We last spoke to him on the show in 2013 about his book, "Madiba A to Z: The Many Faces of Nelson Mandela," which he wrote in conjunction with the film, "Mandela: Long Walk to Freedom."
In 2007, he first joined us to discuss his film, In Debt We Trust: America Before the Bubble Burst and returned as the subprime crisis fueled a financial meltdown. In 2010, he joined us on the release of his documentary about Wall Street called Plunder: The Crime of Our Time.
In 2011, Amy Goodman interviewed Schechter when he stopped by Occupy Wall Street.

subset: commoncrawl


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Relations with Management
(based on 13 ratings in 2016)
"It's very good but depends on the person really. Most people I meet are very helpful and happy to engage but management is generally busy so it can be an issue reaching some people. The mentor I got was wonderful."Graduate, Northampton
"It's a very open and friendly environment in which the door is always open."Supply Chain, Graduate, Stoke on Trent
"There's little focus on feedback but there are reviews every quarter. How accessible your manager is depends on how lucky you are with your placements. I have been exposed to very senior figures in the business and developed important work for them."Graduate, Derby
"I've been warmly welcomed by all levels and have built many relationships throughout the company."Graduate, London
"A high number of senior managers put in a lot of effort with their graduates, but this can conflict with you moving around every year into different projects."Retail, Graduate, Bedford
"The line manager I was assigned hasn't been especially active. However, I have been utilised by another within the business and she has been amazing. She's easy to talk to and I can go to her with any issues or if I require any support. No complaints from that perspective."Graduate, Milton Keynes
"My line manager was previously my mentor. Working from a completely different site he can be a little difficult to get hold of, but generally he is accessible."Graduate, London
"While it obviously depends on the individual, managers are by and large very open and accessible. I have had a formal mentoring relationship with a senior manager and frequently pick up informal advice from other managerial colleagues. Performance feedback is structured and good, and I am praised and recognised for work well done."Graduate, Rugby
"I have found that managers across the company are very accessible and more than willing to help a graduate out."Graduate, Birmingham
"I have regular reviews with my line manager which means we maintain good communication with one another. Other managers on-site are always willing to help me and give me feedback on how I've performed e.g. when I have chaired a meeting. I have a mentor from a different part of the business who is supportive and gives advice/guidance when needed."Graduate, Daventry
"Most managers are really good. Senior managers are usually great communicators, highly competent and real "people" people! The managers I have had have always been keen to develop me in areas that I have stated I am lacking experience or skills, etc."Graduate, Birmingham
"There is a very good management team who make encouraging mentors if you show you are seeking constructive feedback and are career-minded."Graduate, Birmingham
Our 'Inside Buzz' reviews are the comments and views of recent graduate recruits, giving you a view of what it may be like to work for an organisation. Copyright of all TARGETjobs Inside Buzz material lies solely with GTI Media.


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Simplicity 3248; ©1950; Child's Slacks, Shorts, Pedal Pushers and Shirt: The short-sleeved shirt has front-buttoned closing and a notched collar. One breast patch pocket is featured in Style I. Styles II and III have two pockets at lower edges. Slacks, shorts and pedal pushers feature turned up cuffs and two slanted pockets. Saddle-stitching trims the pockets and side seams of the slacks, shorts and pedal pushers.
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Steve Hackett
- Overview
- Biography
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Stephen Richard Hackett, 12 February 1950, Pimlico, London, England. Formerly a member of various minor bands, including Canterbury Glass, Heel Pier, Sarabande and Quiet World, the latter releasing a solitary album on Dawn Records in 1970, Hackett joined Genesis as guitarist in early 1971. He replaced Anthony Phillips, and stayed with the band during their successful mid-70s progressive rock period, appearing on recordings from 1971s Nursery Cryme to the live double album, Seconds Out. By the time the latter was released in 1977, Hackett had left the band. Having previously released his first solo effort, Voyage Of The Acolyte two years earlier, Hackett had decided to pursue a full time solo career. Credited with inventing the two-handed tapping technique, he achieved modest success with a string of albums, including 1980s UK Top 10 Defector, but his following remained largely static and of interest only to die-hard Genesis fans. Subsequent albums...
- MatchSteve Hackett
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A million Virginians have signed up to receive notices from the Department of Motor Vehicles via email, text and phone messages instead of through traditional mail, saving the agency $590,000, Gov. Bob McDonnell said.
"I'm pleased that 1 million Virginians have opted for this environmentally-friendly service that helps save the commonwealth money and resources," McDonnell said in a statement.
The electronic initiative began in August 2010; McDonnell was the first to sign up. Now about one in five Virginia drivers are signed up.
Residents can go to www.dmvNOW.com/myDMV to create an account.


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What do you make of Newsweek's coverage of the Larry King murder?
July 21st, 2008
Dr Petra
Newsweek has as its cover story an account of the tragic Larry King case. In case you haven't heard about this, 15 year old Larry was shot and killed by a male classmate after asking him to be his Valentine. It has led to some violent debates around the issue of young people's sexuality – with some claiming Larry's case as a shocking landmark in gay history, while others arguing it's not a gay rights issue but more about gun control and failing education systems.
Having read the detailed account from Newsweek I'm left feeling that while they have covered the bases there are still problems with the reporting. Perhaps that's because journalists, like all the rest of us, are raised in a predominantly conservative society that at best feels uncomfortable tackling homosexuality, and at worst is homophobic. Which means when you come to cover a story about a sensitive and complex issue, subtle (and not so subtle) biases creep in.
Examples include an opening statement to the piece where Larry was described as dressing 'flamboyantly' and that he had 'hit on a classmate' – phrases that perhaps would not be used (or perhaps would not be so meaningful) if applied to a heterosexual situation. Larry's actions are also listed – not only did he dress flamboyantly, it seems he wore women's clothing, high heeled shoes (that he 'teetered' in) and sometimes more makeup than the 13 year old girls at his school. Clearly alongside describing the case there is a perhaps unintended subtext of blame – Larry dressed on occasion in female clothing and made no secret of finding boys attractive – so the shooting of him after telling another boy he found him attractive is suggested as an inevitable outcome.
Frequently within the piece Larry is referred to as being gay. This is despite lengthy references to his cross dressing and an acknowledgement that he wanted a sex change. A more accurate and helpful description would be transgender – and perhaps rather than seeing Larry as a gay boy the journalists might have explored whether Larry wanted to be a girl. Something he requested from his school when he wanted to be referred to as Leticia (teachers refused to do so).
Clearly this is a dreadful case with devastating effects on Larry's family and friends. Blame is now being apportioned, with a teacher who supported Larry becoming one target (with her sexuality being partly criticised), while perhaps issues of school funding, managing bullying, teacher education, awareness of sexuality and above all gun control are being sidelined.
The Newsweek feature states "kids like Larry are so comfortable with the concept of being openly gay that they are coming out younger and younger". A sentence which again carries the connotation that there's something sinister going on. Most young people who are gay, lesbian, bi, or transgender/sexual are aware of their sexuality from a young age – in the same way as heterosexual kids are aware of their sexual feelings. In many cases young people may not acknowledge or give voice to these feelings because they are afraid to, do not know anyone else who is like them, or have noone they can talk to. We're not seeing some epidemic of young people coming out as though there's been some terrible influence on them to seem gay, what we're seeing is some children feeling either able to express how they feel, or society beginning to wake up to the varied forms of sexuality teens have. I wonder if the same tone would have been used if Larry had been a girl who had told a boy she fancied him and got shot because he didn't feel the same way about her.
It is difficult for many journalists, parents and educators to understand sexuality – particularly how our understanding of sexuality has progressed. Which is why discussions of this issue are often uncomfortable, stilted and unhelpful. Teachers, parents and journalists need support to understand that some kids are gay, some are bi, some are transgender or gender variant. We need to be able to talk about this in a clear, nonjudgemental and frank way.
The writers of the Newsweek piece have done well to bring a shocking story to the public's attention and to try and cover all the angles. They could have done a lot better to try and understand sexuality in a more sensitive manner as it might have strengthened their coverage further.
One excellent resource that should be read by anyone with an interest in this area is Stephanie Brill and Rachel Pepper's The Transgender Child which gives practical advice and information for parents and professionals about children and teenagers.
Sadly cases like Larry King's will continue to happen so long as we muddle being gay with being transgender, and while we continue to apply negative connotations to behaviours that we wouldn't see as a problem in straight kids.Tweet
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Scholar Athletes of the Week
Samantha Henderson
Metairie Park Country Day School
A senior at Metairie Park Country Day School, Samantha has a cumulative GPA of 3.59 and is a member of the Diversity Club, PAX (People Against Xenophobia), and the National Honor Society. She is also a member of both the volleyball and tennis team. She is planning to attended LSU and study business.
Cameron Schwartz
Ridgewood Preparatory School
A senior at Ridgewood Preparatory School, Cameron is a the President and Treasurer of the Key Club, Vice President and Treasurer of the Beta Club, and Vice President of Peer Helpers. Cameron is also a member of the National Honor Society and has a cumulative GPA of 3.9. Cameron participates in many athletics as a shooting guard and point guard for the basketball team, football team wide receiver, cornerback, running back and safety, baseball team left fielder and track team member. In college, Cameron would like to pursue a degree in engineering.


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us on Facebook:
This site is not an official website of the Society of St. Pius X (SSPX). Click here to visit the SSPX home page.
Background and History of St. Anthony's Parish
St. Anthony of Padua Mission, currently located in North Caldwell, New Jersey, was founded by the faithful followers of the late Father Paul A. Wickens, former pastor of the church that was known as St. Anthony of Padua in West Orange, New Jersey. (To read about Father Wickens, his life, and his apostolate, please visit his memorial website.)
Father Wickens' wisdom, leadership, and perseverance were a source of great inspiration to his faithful followers for more than two decades. Over the years, the former St. Anthony's parish grew from a small group to a large, strong, and thriving Traditional Catholic congregation.
All of that changed on July 8, 2004, when Father Paul Wickens passed away after a 6-month fight with cancer.
Those who knew Father Paul Wickens for 10, 15, 20 years or more — and who listened to his sermons every Sunday — remember quite vividly his warnings about the Novus Ordo church. Father Wickens repeatedly warned about the loss of faith and extreme danger to our souls — and to our children's souls — that would result from ongoing association with the Novus Ordo church. He was gravely concerned about the ongoing and widespread homosexual influence that has infested the priesthood and rocked the modern church with its scandals. Constantly warning the faithful members of his flock, Father Wickens particularly singled out the Newark Archdiocese (see file download "Silent No More").
In November 2004, the management (the "Board of Directors") of the West Orange property appointed a new pastor, an employee of the Newark Archdiocese — the same corrupt diocese that Father Wickens had warned against for many years. This appointment was in utter contradiction to Father Wickens' last 20 years of public ministry, and against his explicit wishes for his Chapel (see file download "Cordaro certification") to become a part of the Society of St. Pius X (SSPX).
The appointed pastor subsequently brought in other diocesan priests to the West Orange church. Under this atmosphere of scandal and secrecy — and with the corrupt Newark Archdiocese given spiritual control of the former St. Anthony's parish — it was the last straw for the faithful of that congregation. We knew it was time for us to be in a truly Roman Catholic parish with complete fidelity to the traditional Mass, dogma, and unchanging Magisterium of the eternal Roman Catholic Church.
And so, to carry out Father Wickens' wishes and honor his memory, St. Anthony of Padua Mission of the SSPX was established in Fairfield, New Jersey, and moved to North Caldwell, New Jersey, in August 2006. We invite all faithful Traditional Roman Catholics — and all Catholics who are curious about the eternal and unchanging traditions of the Church — to join us in celebrating the Roman Catholic Traditional Latin Mass at St. Anthony of Padua Mission.
The Truth About the Changes at St. Anthony's in West Orange
This site is maintained by volunteers at no cost to either the Society of St. Pius X or to St. Anthony of Padua Church.


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Related terms for "gc-ms ":
GC-MS analysis companies
, GC-MS sampling companies
, GC-MS column companies
For 40 years, Chromatotec, alliance of the expertise of three companies, Chromato-Sud, airmotec, Medor, has been specialized in the development, manufacturing and sales of online gas analysers by chromatography, exclusively made in France. With offices in China and USA, our group is worldwide known for its leading-edge technology. Based on gas chromatograph principle, ...
based in Val de Virvée, FRANCE.
Chromatographic Specialties is an all Canadian company located in Brockville, Ontario, Canada. For 50 years, we have specialized in supplying top quality Gas & Liquid Chromatography and Sample Preparation consumables and instrumentation to the Canadian scientific community. Chromatographic Specialties has evolved into the largest supplier of products for the Canadian ...
based in Brockville, ONTARIO (CANADA).
Ultra Tech Inc. is a scientific company established in Egypt in 1984 to address the market need for competent, local technical support for analytical laboratory instrumentations. We represent leading-industry companies in the field. To date, Ultra Tech Inc. has supplied over ninety percent of the Ion Chromatography installations in Egypt. Our mission is to provide ...
based in Dokki,, EGYPT.
Torion offers a unique blend of commercial products and technical research, development and engineering services. Our company focus is developing miniaturized instrumentation for on-site, field analysis of chemical and biological threats, contaminants, and pollutants. We achieve this purpose through combining proven technologies in innovative ways in the analytical ...
based in American Fork, UTAH (USA).
Anthias Consulting are experts in the separation sciences in the fields of Gas Chromatography (GC), Mass Spectrometry (GC-MS) and related techniques. Anthias Consulting Ltd provides consultancy and training services to clients throughout Europe, America and beyond, including multi-national corporations, small independent laboratories and academic institutions. We offer GC ...
based in Papworth Everard, UNITED KINGDOM.
Markes International is a specialist manufacturer of instrumentation for detection of trace-level volatile and semi-volatile organic compounds (VOCs and SVOCs). Markes has a well-deserved reputation for its application expertise in the field of thermal desorption, supplying a comprehensive range of instrumentation, sampling equipment and consumables that enhance the ...
based in Llantrisant, UNITED KINGDOM.
MasSpec Consulting Inc is a Canadian GC/MS & LC/MS service company with a primary focus on private sector and government research & analytical labs. Service capabilities include a range of MS instruments: High Resolution Magnetic Sector GC/MS (M-Series AutoSpec), Quadrupole GC/MS, Ion Trap and Time-of-Flight mass spectrometer systems. Our OEM factory training and more ...
based in Oakville, ONTARIO (CANADA).
Shimadzu Scientific Instruments (SSI) is the American subsidiary of Shimadzu Corporation, headquartered in Kyoto, Japan. Shimadzu is a $3 billion multinational corporation with three major divisions: Medical Diagnostics, Aerospace/industrial, and Analytical Instruments. The Analytical Division is one of the world's largest manufacturers of analytical instrumentation and ...
based in Columbia, MARYLAND (USA).
GERSTEL is a family owned company founded in 1967. Its focus is on developing and producing systems and solutions for chemical analysis. Our main emphasis is on automated sample preparation for Gas Chromatography / Mass Spectrometry (GC/MS) and Liquid Chromatography / Mass Spectrometry (LC/MS). No other company has a bigger tool box of sample introduction and preparation ...
based in Linthicum, MARYLAND (USA).
It is now 52 years since Ernest Dawes established Scientific Glass Engineering in a tiny garage at the backyard of a house in Melbourne, Australia. Today, SGE has two modern facilities engaged in developing and manufacturing Chromatography and Analytical Products. SGE has grown substantially with over 400 employees and a distribution and support network in nine countries, ...
based in Austin, TEXAS (USA).
Phytronix Technologies Inc. is a privately owned company based in Québec City, province of Québec, Canada. The company produces and markets laboratory scientific instruments and exports the LDTD Technology around the world.
based in QUEBEC (CANADA).
Should you not already be familiar with CHROMTECH, allow us to take this opportunity to introduce you to our company for analytical instrumentation technology. Our company specializes in the areas of laboratory chromatography and automation. Our product profile includes gas chromatographs, HPLC-equipment, mass spectrometers [MS and MS-MS] as well as auto samplers and ...
based in Idstein, GERMANY.
K`(Prime) Technologies is an authorized sales/service provider for Agilent Technologies, Agilent Genomics, Keysight Nano Measurement, HP/Compaq, and Zip Scientific. In addition, we sell and support instruments from Metrohm, CTC, Frontier and Markes products. We are the largest chromatography sales and service provider in Western Canada with active local sales and support ...
based in Calgary, ALBERTA (CANADA).
Thermo Scientific is a trusted brand that the world's leading researchers, clinicians and scientists count on to solve their analytical challenges. Thermo Scientific brand of Thermo Fisher Scientific, Inc. (NYSE:TMO), we help scientists meet the challenges they face in the lab or in the field every day. From routine analysis to new discoveries, our innovations help ...
based in MASSACHUSETTS (USA).
Spectron, Inc. has been manufacturing and supplying quality cones and consumables to the ICP & ICP-MS community worldwide. With extensive experience in ICP-MS and ICP-OES techniques, we bring a unique level of expertise to the manufacturing process. Spectron manufactures sampler and skimmer cones for all the major brands of ICP mass spectrometers including Agilent, GBC, ...
based in Ventura, CALIFORNIA (USA).
Dr. James Meinhard was asked in 1973 if he could fabricate a concentric glass nebulizer for the inductively coupled plasma spectrometer, then in its infancy. Patterning his efforts after a century-old design (Gouy 1879) and working in a private shop, Dr. Meinhard drew capillaries and sealed them into prefabricated shells. The resultant MEINHARD concentric glass nebulizer ...
based in Golden, COLORADO (USA).
Quantum Latvija LTD offers in the Latvia`s market equipment for manufacturing, research and analysis, laboratory supplies and services, equipment maintenance, reagents and reference materials. Quantum Latvija LTD is official distributor of Agilent Technologies in Latvia.
based in Riga, LATVIA.
For more than 40 years, Agilent Technologies has been the leading provider of solutions for the detection, identification, and characterization of chemical and biological compounds. From environmental testing to semiconductor manufacturing, customers rely on Agilent to help them solve analytical problems with accuracy and precision. Agilent`s deep industry knowledge, ...
based in Santa Clara, CALIFORNIA (USA).
Quantum Analytics is a value-added distributor for multi-vendor, analytical instrument systems specializing in renting and leasing. We provide attractive financial plans for acquiring new and refurbished analytical instrumentation. Rent, lease or purchase from our multi-million dollar inventory and have it in your lab the next day. Quantum is a major US distributor for ...
based in Foster City, CALIFORNIA (USA).
Alpha Omega Technologies (AOTI) is a premier provider of new chromatography instruments and related accesories for laboratories in the US and abroad. Our staff of application engineers and chromatographers start with instruments from industry leading manufacturers, configuring each system to the particular need of each customer.
based in Brielle, NEW JERSEY (USA).
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Janki Puram,civil Lines, Near Kapoor Nursing Home, Jhansi, Uttar Pradesh 284001
Locate a Tata Dealership in Jhansi. CarDekho.com makes it easy to find authorized Tata dealerships and showrooms in Jhansi. Locate over 1Tata Dealers in Jhansi. We aim to provide you with all important contact details about the Tata car dealers in Jhansi at your convenience. Details of which are listed above:
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Healthy Breakfast Bites: Apple Pie Overnight Oats ...
There's nothing more satisfying and stress-free than waking up to a delicious, pre-made breakfast – and this apple pie...39
There's nothing more satisfying and stress-free than waking up to a delicious, pre-made breakfast – and this apple pie...39
Who has time to make a nutritional breakfast meal each morning? Prep these incredible bars overnight and they'll...40
Embrace the warmer weather and make sure your breakfast is packed with seasonal fruit and vegetables this...136
Looking for a tasty way to enjoy your granola in the morning? From granola cups to fruity...51
This egg frittata is perfect for breakfast, brunch, or let's face it – any time of the day!...38
Who doesn't love a fresh punnet of blueberries on a lazy Sunday morning? This recipe takes tasty ricotta...677
Indulge in a deliciously healthy breakfast of bircher muesli, which is perfect for a busy working morning. Simply prepare...18
Who can resist a delicious English breakfast, especially when the weather takes a turn for the worst...43
We know you don't have a lot of time in the morning for a healthy and delicious...546
Why not take advantage of the Easter long-weekend and bake your family and friends some delicious homemade...11
Who can resist a perfectly poached egg, especially when it's served with smashed avocado, hollandaise sauce and...24
The initial thought of a crepe might bring about a mental image of sugar, chocolate, with a...368
Fuel your body with a delicious mixture of chia seeds and oats first thing in the morning. Chia...23
Start your morning the right way with a healthy wild mushroom omelette which is sure to keep...14
Did you know that the first mention of French toast was in the 14th century? This traditional...33
Want to create a delicious and healthy breakfast snack which you can eat while you're on-the-go? We have...32
There is no denying that eggs make the perfect breakfast recipe; packed with protein, and so quick...205
If you're constantly on-the-go and can't manage to prepare a healthy breakfast that doesn't come in a...35
Make this healthy breakfast or brunch meal which is filled with protein and will keep you full until...23
More than just your typical ham and cheese toastie, a croque-monsieur is a delicious snack perfect for...28
Who can deny the delicious taste of avocado early in the morning? Certainly not us! Avocado toast...23
Start you day with a wholesome meal of baked eggs – with a twist! Add a bunch of...36
Don't let your next smoothie taste flat and lifeless, especially if you're looking to stay on the...17
Enjoy a delicious breakfast packed with natural fibre and sesame seeds as an essential way to start...17
A breakfast sandwich? This delicious meal might bring negative connotations such as high in sugar, high in...5


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species Pablo Muñoz-Rojas, Claudio Quezada- Romegialli & David Véliz Conservation Genetics Resources ISSN Volume 4 Number 2
- Phillip Pearson
- 2 years ago
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1 Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with crossamplification in seven Trichomycterinae species Pablo Muñoz-Rojas, Claudio Quezada- Romegialli & David Véliz Conservation Genetics Resources ISSN Volume 4 Number 2 Conservation Genet Resour (2012) 4: DOI /s
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3 Conservation Genet Resour (2012) 4: DOI /s TECHNICAL NOTE Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species Pablo Muñoz-Rojas Claudio Quezada-Romegialli David Véliz Received: 28 October 2011 / Accepted: 9 November 2011 / Published online: 20 November 2011 Ó Springer Science+Business Media B.V Abstract A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1 13 alleles per locus) and the observed heterozygosities ranged from 0 to Hardy Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae. Keywords Microsatellite markers Population genetics Trichomycterus areolatus Cross-amplification Introduction Freshwater fauna are among the most threatened taxa of the planet (Ricciardi and Rasmussen 1999; Saunders et al. 2002). In Chile, 64% of the freshwater fish are in danger of extinction and 29% are vulnerable or insufficiently known (Habit et al. 2006; Vila et al. 2006); currently there are few effective protection measures for these organisms. One of these threatened species is the river catfish Trichomycterus areolatus (Valenciennes 1840), which is P. Muñoz-Rojas C. Quezada-Romegialli D. Véliz (&) Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile P. Muñoz-Rojas C. Quezada-Romegialli D. Véliz Instituto de Ecología y Biodiversidad, Facultad de Ciencias, Universidad of Chile, Casilla 653, Santiago, Chile widely distributed in Chile from Huasco ( S) to Chiloe ( S) (Arratia 1981; Dyer 2000). This species has conservation problems, as do all the species of the subfamily Trichomycterinae in Chile (Habit et al. 2006; Vila et al. 2006; Quezada-Romegialli et al. 2010). In order to improve the plans of population conservation, we describe here the isolation and characterization of ten polymorphic microsatellite loci for the Chilean Andean catfish T. areolatus. Total genomic DNA was extracted from ethanol-preserved fin clips using the salt-extraction method (Aljanabi and Martinez 1997) from samples collected from the Limari watershed (Los Aromos: S), Choapa watershed (Choapa Pueblo: S) and Maipo watershed (Estero Puangue: S). The library was developed by ATG Genetics (Vancouver, BC, Canada). Template DNA was digested by the restriction enzyme HaeIII and the microsatellite enrichment by biotin capture of dinucleotide (TC n and GA n ) microsatellites. The first 36 plasmid clones containing a dinucleotide motif were purified and amplified by PCR. Seventeen primer pairs were designed using Primer5 and tested in our laboratory, of which ten gave reliable amplifications and polymorphic markers using agarose gel electrophoresis. In order to evaluate polymorphism in an automatic sequencer, reverse primers of each microsatellite were marked with a fluorescent dye. Polymerase chain reaction (PCR) amplification mixtures (12 ll) contained 100 ng template DNA, 0.25 lm ofeach primer, 100 lm of each dntp, 2 mm MgCl ll 10 9 PCR buffer and 0.5 U Taq platinum Polymerase (Invitrogen). Cycling conditions consisted of an initial denaturing step of 3 min at 95 C, followed by 35 cycles of 30 s at 95 C, 30 s at the specific temperature, 1 min 30 s at 72 C, and a final elongation step at 72 C for 5 min. PCR products were genotyped in Macrogen Inc (http://www.macrogen.com)
4 444 Conservation Genet Resour (2012) 4: Table 1 Primer sequences and characteristics for ten microsatellite loci for Trichomycterus areolatus, including their polymorphism in three populations (LA, Los Aromos S W; CC, Choapa S W; PU, Puangue S W) Locus Primer sequence ( ) Repeat motif Tm ( C) GenBank accession no. Sample N Ho/He N A Size range (bp) Taredi1 F: GAATTCTGAGTGCAGAGCTCT (CA) 9 55 JN LA R: CATACCAGCTGTGAAATTCATACA CC PU / Taredi4 F: TTTTGAAACCGTCGCTGGAC (GT) JN LA / R: CTGTCCTCAGGCTCTTTAC CC PU 6 083/ Taredi6 F: TTTTCAGCTCACCACAATGC (TG) JN LA R: GCTGCGTCACATGGTGTTAT CC PU 4 0/ Taredi10 F: CCGTGTTGTTTATTCGAGCTC (CA) 9 55 JN LA / R: GCAGTTATCAGCTGAACCAG CC / PU / * Taredi11 F: CGAGAGCGAGATCGTGTGTA (CA) JN LA / R: TCATAAATCAACGATGCGTCA CC / PU Taredi14 F: TCTTACAGTATTTAACATCCTGG (CA) JN LA / R: GAATTCAGTTCAGCTTTATTCAC CC /0.81* PU / Taredi17 F: CATAAACTGTGTATGTGTGTCA (GT) JN LA R: GGGGTTCGTTTTCAGAACTG CC PU 12 0/0.38* Taredi21 F: AACCACAAGGTTTGAGCGTC (AC) JN LA / R: AACACTGCTGCATAGACCAG CC / PU Taredi212 F: GGTCTGGAATAAAAATTACAAACACA (GA) JN LA / R: ACCGAACAGGTGTCACCATC CC / PU /0.86* Taredi24 F: CCAAGTGGAGCTGCTAGAAA (GT) 20 (CA) 9 55 JN LA / R: CTGCACGCTCCTTTTCCAAA CC / PU 8 0/ Tm annealing temperature, N number of analyzed individuals, NA number of alleles; * P \ 0.01 for significant departures from HWE, tested after 3,000 permutation in Genetix (Belkhir et al. 1996) using the internal size standard LIZ 500 (Applied Biosystems). Clone sequences were published in Genbank with the following accession numbers: JN to JN (Table 1). In the 63 individuals from three geographically isolated watersheds of the central zone of Chile we observed between 1 and 13 alleles per locus (Table 1); some alleles were found in all three watersheds. The Los Aromos site (LA, Limarí watershed) had the least allele diversity (1 6 alleles per locus), while Choapa (CC, Choapa watershed) had from one to 8 alleles per locus and Puangue (PU, Puangue watershed) presented the greatest allele richness (1 13 alleles/locus) (Table 2). Deviations from Hardy Weinberg expectations (HWE) were calculated using the permutation test associated with the F IS calculation performed with GENETIX software (Belkhir et al. 1996). Significant deviations from HWE were observed only in three out of ten loci in the Puangue River (Taredi10, Taredi17 and Taredi212) and one from Choapa (Taredi14). MICROCHECKER software (Oosterhout et al. 2004) was used to identify genotyping errors due to null alleles, large allele dropout and scoring of stutter peaks. The analyses suggested that null alleles may be present in three loci in Puangue (Taredi10, Taredi17 and Taredi 212) and one locus from Choapa (Taredi14). Furthermore, no significant linkage disequilibrium was detected among pairs of the
5 Conservation Genet Resour (2012) 4: Table 2 Number of alleles from cross-species amplification in seven South American catfishes Species N Locus (Taredi) Trichomycterus punctulatus Valenciennes, Trichomycterus aff rivulatus Valenciennes, Trichomycterus laucaensis Arratia, Trichomycterus chungaraensis Arratia, Trichomycterus cf chiltoni (Eigenmann, 1927) Bullockia maldonadoi (Eigenmann, 1928) Hatcheria macrei (Girard, 1855) N is the number of individuals tested studied loci, indicating that the loci are probably not closely linked on chromosomes; thus the loci may be considered as independent markers. In addition, the analysis of cross amplification using 23 individuals of 7 other species of South American catfish found amplifiable loci in all species (e.g., Taredi1) and others which did not amplify (e.g. Taredi11 y Taredi21). Species of the same genus amplified from 3 to 7 of the ten loci analyzed; Hatcheria macrei was the species which showed the smallest number of amplified loci (N = 2), which is expectable in this type of cross amplification given that the phylogenetic relations among the species are not clear. Interestingly, 5 of the 10 loci amplified in Bullockia maldonadoi, suggesting that this genus may be closer to the species T. areolatus than to the genus Hatcheria. These results show the utility of these 10 independent markers to devise plans of genetic conservation for populations of T. areolatus which are currently threatened, and will potentially be useful for other species of the family Trichomycteridae. Acknowledgments We thank C. Newton and ATG Genetics, M. Espinoza for lab assistance and L. Eaton for reviewing the English version of the manuscript. This study was supported by Fondecyt to DV. DV also thanks Grant PFB-23 (CONICYT, Chile) and Grant ICM P PM thanks IEB master grant and CQR thanks CONICYT doctoral grant. References Aljanabi SM, Martinez I (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25: Arratia G (1981) Géneros de peces de aguas continentales de Chile. Museo Nacional de Historia Natural Chile, Publicaciones Ocasionales 4: Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996) Genetix 4.05, Logiciel sous Windows pour la Génétique des Populations. Laboratoire Génome, populations, interactions, CNRS UMR 5000, Université de Montpellier II, Montpellier Dyer D (2000) Systematic review and biogeography of the freshwater fishes of Chile. Estud Oceanol 19:77 98 Habit E, Dyer B, Vila I (2006) Estado de conocimiento de los peces dulceacuícolas de Chile. Gayana 70: Oosterhout C, Hutchinson W, Wills D, Shipley P (2004) MICRO- CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4: Quezada-Romegialli C, Fuentes M, Véliz D (2010) Comparative population genetics of Basilichthys microlepidotus (Atheriniformes: Atherinopsidae) and Trichomycterus areolatus (Siluriformes : Trichomycteridae) in north central Chile. Environ Biol Fishes 89: Ricciardi A, Rasmussen J (1999) Extinction rates of North American freshwater fauna. Conserv Biol 13: Saunders D, Meeuwig J, Vincent A (2002) Freshwater protected areas: strategies for conservation. Conserv Biol 16:30 41 Vila I, Pardo R, Dyer B, Habit E (2006) Peces límnicos: diversidad, origen y estado de conservación. In: Vila I, Veloso A, Schlatter R, Ramírez C (eds) Macrófitas y vertebrados de los sistemas límnicos de Chile. Editorial Universitaria, Santiago de Chile, pp
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Designed by Turi Gramstad Oliver for the iconic Figgjo "Astrid" series, this piece is definitively Scandinavian! Figgjo produced some of the most sought after Norwegian ceramic work from the Mid Century, and like other purveyors of Scandinavian Modernist design aesthetics, Figgjo work tends to be a combination of whimsy and color. With its silkscreened blue and red line work, this piece is no exception to that rule, creating a look that is both focused and fun. Measuring 8" tall, this pitcher is in great vintage condition with no chips, cracks or crazing. Marked "turi-design, astrid, made in norway" on its bottom, this pitcher would be a perfect addition to any mid century Scandinavian collection!
Danish Modern Figgjo Astrid Pitcher Designed by Turi Gramstad Oliver - Mid Century Scandinavian Vase
- Vintage item from the 1960s
- Material: ceramic
- Ships worldwide from United States
- Feedback: 495 reviews
- Favorited by: 6 people
- Listed on Jun 20, 2012
- 6 favorites


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Solo exhibition - ako Castuera
George Takei interview
July 29th at GIANT ROBOT AND GR2
Solo Exhibition
Sold Out
Ako Castuera Solo Exhibition at GR2 Sat July 29, 2017
July 15, 2017
More information TBA!
Read more →
Go Get 'Em Tiger Art Show at GIANT ROBOT STORE! Begins July 29th, 6:30pm
Go Get 'Em Tiger Art Exhibition at GIANT ROBOT STORE Coinciding with Ako Castuera's Solo Exhibition at GR2 and the WLA Obon just one block away is Go Get 'Em Tiger Exhibition at GIANT ROBOT STORE curated by Cassia Lupo! You will see a themed exhibition including the following great...
GR X SDCC Mari Inukai Original Drawings
July 14, 2017


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From the Four Corners go west on M134 (approx 4 miles) to the junction of Pike Bay Road, turn left, proceed about 1 mile and on your right you will see the marshy Pigeon Cove Flooding. Look for deer, birds, and bears in the rushes. The kids will enjoy going through the tunnel, just past the floodings.
If you like to travel through the forest, follow Pike Bay Road (1.1 mile) to the main junction, turn right to Noble's Road (1.1 mile)'s- back track to the main junction, turn right on Pike Bay Road (.4 miles)-back track to main junction, turn right onto Cream City Point Road (2.4 miles) to the causeway- all of this is paved and hardly a house to see. At the causeway, go forward to Gravel Lake (2.1 miles) on the hard packed gravel road.- then back track. A main point to remember, on all of the named roads you will have to back track. Pick up a detailed map at the Visitor Center at the Four Corners. There are many birds including Great Horned Owls in the area, plus you get glimpses of Lake Huron. This is a great area to visit just before dusk searching for the elusive bear.
If you like to bike, park you car on the inland side of the road at the junction of M134 and Pike Bay Road. Riding only the paved roads mentioned you will travel 10 miles, add the unpaved road and you get another 4.2 miles. By car or bike this is a round trip of 14.2 miles. For the bikers the traffic is minimal and you're going through your own private wilderness.


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Look fashionable by tiring this blue dress and exhibit a tidy and sparkling gaze in your next gathering. Match up with high heels and shiny clutch to get the best look. It is light mass and stretchable.
Sizes Available:
All Sizes. 0 to 36W
Custom Sizing
Style Options:
As it is Dress-Length-Knee Length, Below Knee, Mid Calf Length Sleeve Type-Short Length, Elbow Length
Fabric and Color:
Denim and Blue
Washing Instructions:
Iron on reverse, Do not bleach, Tumble dry, Dry cleanColor may vary slightly due to the color calibration of each individual monitor.
Step 1: Choose Standard Size or Custom Size
Step 2 : Give Your Height
Step 3 : Opt for Neckline, Sleeve, Length Changes It is so easy!


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Give us a call 0845 468 0698
High Ball Table Lamp
The bluey-grey finish of this Stoke made lamp is a bit special. We love the way the glaze gathers in each groove to give a deeper hue when fired in the kiln.
Why we love our High Ball Table Lamp
Ceramic base handmade for us in Stoke-on-Trent
Lovely high-gloss glaze
Add one of our elegant lampshades made by Tim in his Dorset workshop
Dimensions & need to know
- Recommended bulb (sold separately): B22 Bayonet Cap, 13-15 watts Compact Fluorescent (CFL)
- Find out where you can try this out.
Meet the makers
Delivery for this small-sized item
#1 Order
Get excited…
#2 Bbbbbrrriinngg
…then we're good to go
#3 Ding dong
Your goodies arrive in a few days!
Delivery Cost £4.95
Please note we don't deliver to non-mainland UK addresses.
Quibble-free 14-day returns
If it's not quite right just pop it back in the post to us and we'll do the rest. Easy.
Come on it's a no-brainer!Click here for our returns policy
Hassle free returns
If you're not 100% happy, please follow these simple steps:
1. Ping us an email at firstname.lastname@example.com quoting your sales order number or call 0845 468 0698 within 14 days of delivery.
2. We'll issue you with a sales return number and return address.
3. Pop it back to us in the post wrapped as securely as possible. We recommend a recorded mail service that's happy to handle packages of all sizes.
We'll refund you in full and if it's the full order you're returning we'll refund the original delivery charge too. Please note we do not cover the cost of posting back the item.
For hygiene reasons we cannot take back mattresses once their protective wrapping has been opened. So do make sure you have a bounce on it before you take its kit off!


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Hike & Bike Trail
The West Feliciana Parish Sports Park Hike and Bike Trail and Archery Range is open to all users.
Horses are not allowed on the trail or on the trail bridges.
All users sign in before entering at the designated area located at the trail head.
All bike riders must wear a helmet while on the trail.
The trail and archery range is used at your own risk.
Hours of the trail 7 AM - Dusk unless otherwise noted.
All volunteer trail and archery range maintenance must be authorized and coordinated by the West Feliciana Parish Parks and Recreation Park Operations Department.
A trail/archery range volunteer agreement form is required of anyone who wishes to do trail or archery range maintenance for the department.
HIKE & BIKE TRAIL MAP - Download the PDF
Thank you to New Orleans Fox8 WVUE for this news segment on the Beast.


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Phi is a family of organic aesthetic lamps that evokes the classic Scandinavian design.
Designed by David Abad for B.lux, it offers warm and comfortable lighting which, because of the aesthetic and modular characteristics it recreates, forms lights and shades of great character. Its timeless and organic design allows the Phi lamps to be installed in any type of space, in both classic and contemporary interior design.
Scandinavian-inspired wall lamp featuring metallic structure and ABS. Finished in matt white. 33 cm x 41 cm Halog. ECO max. 100W (E27) Fluor. glob. max. 23W (E27) LED (E27)
Manufactures Unlike design editor companies, the B.Lux Group has its own manufacturing plant, which allows us to incorporate the highest manufacturing processes into our products.


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emma jagu
École: ESCP Europe Business School, CentraleSupelec
Major: Master in Management (and Engineering coursework)
Languages: French, Dutch, German, and English
Location: Paris, France
Download Emma's Resume
Biography: I grew up in a small town in Normandy, France, and the first language I learned was Dutch – my mother's native tongue. Once in school, I learned French, German and English. I consider myself a citizen of Europe and I am passionate about its progress, especially in the field of energy. My dream is to one day lead my own firm and to become a major actor of the low-carbon economy.
I am combining my technical knowledge and management skills through a double-diploma in Electrical Engineering in the French Engineering School CentraleSupélec and in Management in the European Business School ESCP Europe.
To me, the most important task for leaders in the 21st century is to ensure a livable planet for future generations, and that is why I believe that a deep transformation of the energy market is necessary. I want to attain global influence in that area to use it to encourage sustainable energy transition worldwide. I believe that every human deserves clean and sustainable energy.


text: Celebration is one of the biggest joys of the holiday season. From gathering together with family and friends, to sitting in reflection at the new year, to watching your child fall fast asleep under the Christmas tree. Traditions are made with each passing year, and the enjoyment of creating new ones is always a blessing.


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House Sitter in Bradenton
I can do almost anything you put me up to like get the mail water plants and more just ask me and I will reply back
House Sitter Details
|Email Address
|Phone Number
|941-730-... Confirmed
Collect Mail
Feed Pets
Care For Plants
Take Out Trash/Recycling
Notes made here will only be seen by you. If you want to message the owner of this listing
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2002 Mercedes-Benz M-Class - Consumer Review
Average Consumer Rating
Total Reviews
ML500 4dr SUV (5.0L V8 AWD 5-speed Automatic)
Build Quality
Reliability Value
Build Quality
Reliability Value
4 of 5 people found this review helpful
2002 ML 500 - great to drive awful to maintain
By jleneveu on
Vehicle2002 Mercedes-Benz M-Class ML500 AWD 4dr SUV (5.0L 8cyl 5A)
ReviewSafety, performance (fun to drive), all weather drving capability - wonderful vehicle.
Maintenance costs on the other hand are unacceptable for a vehicle of the this class. Problems, you bet - 4 rear main engine seals in 96,000 miles at $1,300 per repair, front lower ball joints at 25,000 miles, engine oil leaks, interior plastic trim pieces breaking, $775 for brakes every 22,000-25,000 miles, three door panels repainted - rust problems, $1100 to repair ventilation system, and many more.
Best FeaturesHandling - very good for a SUV though sway bars are a little stiff. AWD drive system is great for Colorado weather. Engine and transmission are smooth and powerful - this SUV gets going and it weighs over 5,000 lb!
Worst FeaturesJust find a way of kkeping the vehicle out of the shop - way to expensive to maintain and repair.
Recommend (4) (1)
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Holland & Rees: Nursing Evidence-Based Practice Skills
Flashcard glossary
Test your knowledge of the important terminology using these interactive flashcards. First, look at the term on the card and try to work out the definition. When you are ready, press the button to 'flip' the card and see if you were right.


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Justin Timberlake's media takeover has been swift and successful since he announced in January that he was dropping a new album, but the sudden timing of the pop star's promotional blitz has some insiders wondering if JT was cornered into his triumphant return to music. Get the inside scoop in today's lead story from San Francisco!
Join The Conversation
Anyone who really believes Justin Timberlake was forced into making music knows nothing about him. He would not make music if he didn't want to. It means too much to him. Some bloggers have really come up with some very stupid things since he's made such a successful return.
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JCP to consider 7 names for appointment of additional judges of SHC
ISLAMABAD—Chief Justice of Pakistan Justice Anwar Zaheer Jamali has summoned a meeting of the Judicial Commission of Pakistan on June 6 to consider recommendations of seven names for appointing additional judges of the Sindh High Court. Chief Justice Sindh High Court Justice Sajjad Ali Shah has recommended seven names including a District and Sessions Judge Rasheed Ahmed Soomro, Advocate Arshed Hussain Khan, Advocate Masood Khan, Advocate Saleem Jessar, Advocate Abdul Hamid Barakzai, Advocate Hamayoon Khan and Advocate Sarfaraz Akhund for the appointment of additional judges of the High Court.
Meanwhile, the Chief Justice of Pakistan has also convened a separate special meeting of the Judicial Commission on June 6 to consider proposed amendments in Rules 2010 of the Judicial Commission of Pakistan.


text: Advanced Photonix, Inc. ® (NYSE Amex: API) today announced that it has signed a value-added reseller (VAR) agreement for its T-Gauge™ industrial terahertz gauging system with Thermo Fisher Scientific for its line of Thermo Scientific web and metal gauges. The T-Gauge™ measurement system can be used for on-line or off-line measurements for quality assurance and process control of web based and converting industries. API is developing a VAR partner network to enable T-Gauge™ industrial process control solutions for continuous web, discrete manufacturing and anomaly defect inspection applications. The company plans to develop a VAR network which will serve vertical markets where VARs have unique subject matter expertise. VAR partners are trained to sell, install and support API T-Gauge™ measurement products. Each VAR will have an in house application development T-Gauge system to perform feasibility studies and develop new market applications for non-contact measuring of physical properties such as; multiple layer thickness measurements, density, contamination by foreign substances, sub-surface defects within a product and moisture analysis. The technology is very adaptable to different sensor configurations, including robotic controls, to support many different applications. The resulting innovative gauge will help manufacturers to improve quality and reduce costs. Richard Kurtz, President and CEO commented, "API is developing and building a VAR partner network and we are pleased to have established this relationship with Thermo Fisher Scientific, which has a long history of investing in new technologies for non-destructive measurement for manufacturing process control, including the introduction of the first beta gauge for basis weight measurements. Thermo Fisher provides a global presence with world class customer support and market knowledge, which we believe will lead to more rapid market adoption of our terahertz T-Gauge™ platform." Douglas Wright, Global Commercial Manager for Gauging at Thermo Fisher Scientific added, "We are delighted about this new relationship with Advanced Photonix. Their new sensor technology compliments our Thermo Scientific measurement and control product portfolio to address a wider range of web and metal coating applications. It supports our company strategy to meet customers' requirements for new and more advanced instruments and process solutions."


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Curt Arnold <carn...@apache.org>
|Re: XMLSocketAppender
|Wed, 06 Dec 2006 00:50:20 GMT
On Dec 3, 2006, at 4:28 PM, Marc Rossi wrote: > > Went ahead and added the following line to the end of the > XMLSocketAppender::renderEvent() method: > > os1->write(output) > > and it is now sending out the xml event info (although it still isn't > displaying properly in chainsaw). Not sure why this line wasn't > already > there (almost the same line was commented out in the original source). > Logged and resolved as http://issues.apache.org/jira/browse/ LOGCXX-164. Also logged https://issues.apache.org/jira/browse/ LOGCXX-165 as the original 0.9.7 and the current implementation could result in corrupted XML due to a mismatch of the expected UTF-8 encoding and the actual encoding, however fixing that would probably warrant a complete review of SocketAppender and XMLSocketAppender in log4cxx. The code using the Microsoft Platform SDK macro T2A was removed during the migration to supporting both single char and wide char logging simultaneously. It just wasn't restored when things were put back together.
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