text: Sarah McCammon Sarah McCammon worked for Iowa Public Radio as Morning Edition Host from January 2010 until December 2013. The Uninjured Victims Of The Virginia Tech Shootings By Sarah McCammon • Apr 14, 2017 This weekend marks 10 years since the deadliest campus shooting in U.S. history, at Virginia Tech. Thirty-two people were killed and many others injured on April 16, 2007, on the campus in Blacksburg, Va. Still others witnessed the shooting and were physically unharmed, but carry psychological wounds that they've also spent the last decade recovering from. The Unsung Equestrian Heroes Of World War I And The Plot To Poison Them By Sarah McCammon • Apr 6, 2017 April 6 marks 100 years since the U.S. Congress voted to declare war on Germany, entering World War I. The war took the lives of 17 million people worldwide. What's not as well-known is the role that animals played at a time when they were still critical to warfare. Redistricting Reform Advocates Say The Real 'Rigged System' Is Gerrymandering By Sarah McCammon • Mar 18, 2017 If the election results of 2016 were really about rejecting the political establishment, then Congress didn't get the memo. After all, 97 percent of incumbents in the U.S. House of Representatives seeking re-election won even as national polls show overwhelming disapproval of Congress. Public Clinics Fear Federal Cuts To Planned Parenthood Would Strand Patients By Sarah McCammon • Mar 7, 2017 Opponents of abortion rights have long argued that public funds for services like cancer screenings and contraception should go solely to health clinics that don't provide abortions. Trump Skips Gridiron Dinner As His Staffers Get Roasted By Sarah McCammon • Mar 5, 2017 Vice President Pence came to the annual Gridiron Dinner looking ever-so-slightly underdressed. "I thought I'd be OK wearing a black tie tonight," he joked. "Then Nancy Pelosi asked me to refill her coffee." Pages« first ‹ previous 1 2 3 4 … next › last »


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''I have decided to retire from international cricket immediately,'' the left-arm spinner, who was recalled for the first Test against India, said in a statement.
''I am accordingly unavailable to form part of the Proteas squad for this season,'' added the former Protea vice-captain.
A veteran of 43 Tests and 115 ODIs is sought by the Delhi Police for his alleged involvement in the 2000 match-fixing scandal which rocked the cricketing world.
Fearing arrest, he refused to tour India after his name came into the spotlight along with the then South African captain Hansie Cronje and opener Herschelle Gibbs.
Boje did not give any specific reason for his sudden decision and said that he wanted to continue in domestic cricket.
''I have had 11 years of international cricket and will always cherish wonderful memories of playing for South Africa. I now feel that the time has come for me to devote the remainder of my playing years to Eagles cricket,'' he said.
''I wish my former teammates in the Proteas side the very best for the future and Im confident that the team has a very bright cricketing future,'' he added.

subset: commoncrawl


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Susceptibility of neuron-like cells derived from bovine Wharton's jelly to bovine
Virology and prion diseases
Bovine herpesvirus type 5 (BoHV-5), frequently lethal in cattle, is associated with significant agricultural economic losses due to neurological disease. Cattle and rabbits are frequently used as models to study the biology and pathogenesis of BoHV-5 infection. In particular, neural invasion and proliferation are two of the factors important in BoHV-5 infection. The present study investigated the potential of bovine Wharton's jelly mesenchymal stromal cells (bWJ-MSCs) to differentiate into a neuronal phenotype and support robust BoHV-5 replication.
Upon inducing differentiation within a defined neuronal specific medium, most bWJ-MSCs acquired the distinctive neuronal morphological features and stained positively for the neuronal/glial markers MAP2 (neuronal microtubule associated protein 2), N200 (neurofilament 200), NT3 (neutrophin 3), tau and GFAP (glial fibrillary acidic protein). Expression of nestin, N200, β-tubulin III (TuJI) and GFAP was further demonstrated by reverse transcriptase polymerase chain reaction (RT-PCR). Following BoHV-5 inoculation, there were low rates of cell detachment, good cell viability at 96 h post-infection (p.i.), and small vesicles developed along neuronal branches. Levels of BoHV-5 antigens and DNA were associated with the peak in viral titres at 72 h p.i. BoHV-5 glycoprotein C mRNA expression was significantly correlated with production of progeny virus at 72 h p.i. (p < 0.05).
The results demonstrated the ability of bWJ-MSCs to differentiate into a neuronal phenotype
The two most basic properties of stem cells are their capacity for indefinite self-renewal and differentiation into multiple cell or tissue types
Neurons derived from embryonic stem cells express neurofilaments, neuron-specific class III β-tubulin (TuJl) and a number of neuron-specific microtubule-associated proteins
The neurotropic RNA and DNA viruses that produce central nervous system (CNS) diseases employ a range of pathologic mechanisms in a variety of hosts. The useful biological models applied to isolate, propagate, and study biological properties of these viruses include mice, rabbits, monkeys and hamsters. The α-herpesviruses, including human herpes simplex types 1 and 2 (HSV-1 and HSV-2), varicella-zoster virus (VZV), animal pathogens such as pseudorabies virus (PRV), bovine herpesvirus types 1 and 5 (BoHV-1 and BoHV-5), and Marek's disease virus are neurotropic viruses capable of invading the peripheral and central nervous systems to cause neurological disease
BoHV-1, associated with respiratory and genital disease, and BoHV-5, associated with neurological disease, cause significant agricultural loss.
In contrast with
Differentiation of stem cells into neuron-like cells
The main objective of the present study was to assess the full neural potential of bWJ-MSCs after culture in a retinoic acid-deprived reprogramming medium. Continuous culture for 28 days resulted in cells exhibiting varied morphologies, including cells with multiple long thin-axon-like processes at 7, 14, 21 and 28 days (Figure
Figure 1
Bovine Wharton's jelly differentiation
Bovine Wharton's jelly differentiation. Photomicrographs representative of the morphological appearance of neuron-like cells under differentiation at 7, 14, 21 and 28 days of neuron induction observed under phase contrast microscopy (x 40 magnification). The multipolar, round cell bodies form a network-like structure. The inset images show the undifferentiated bWJ-MSCs.
Figure 2
Neuron-like cells characterization
Neuron-like cells characterization. After 28 days of neurogenic induction, a neuron-like morphology was visible under phase contrast microscopy. A) Neuron-like cells with triangular, round and cone-shaped morphology; B) Bi-polar neuron-like morphology; C) Network among neuron-like cells; D) Long axon of a Golgi neuron;x 100 magnification; E) RT-PCR analysis of neuronal specific markers:nestin, N200, β-tubulin III, GFAP and NT3 transcript expression in neuron-like cells after differentiation were observed. The H2A gene was used as an internal control for the reverse transcription-polymerase chain reaction. MW-molecular weight 1 kb plus.
Figure 3
Neuron-like cells surface markers
Neuron-like cells surface markers. Immunofluorescence images showing the expression of neuronal markers, such as CXCR4, GFAP, NT3, nestin, MAP2, Tau, ubiquitin, N200, and TuJI, by immunofluorescence staining. DAPI indicates the nucleus. Inset images show the absence of neuronal markers in the undifferentiated bWJ-MSCs (scale bar: 20 μm).
Characterization of differentiated neuron-like cells
The distribution of neural/glial cell markers in differentiated cells is shown in Table
Figure 4
Neuron-like cells phenotype
Neuron-like cells phenotype. Flow cytometric analysis of surface marker expression after neuronal induction of bovine-derived UC-WJ cells. The data from the negative isotype control and the respective surface markers for mesenchymal stromal cells are represented by the histogram. The data shown are representative of the cell phenotype after neuronal induction. The data were processed using an Attune™ acoustic focusing cytometer, and auto-fluorescence was excluded as a global compensation tool (≤ 103). The y-axis represents a log scale.
Susceptibility of neuron-like cells to BoHV-5 infection
The susceptibility of neuron-like cells to BoHV-5 infection was investigated by assessing the viral growth curve, cell viability, and cytopathology. The one-step kinetic growth curve profile for infected neuron-like cells was established. CPE are an important parameter in the
Figure 5
Figure 6
Identification of BoHV-5 infection
Identification of BoHV-5 infection. Detection of BoHV-5 antigens, viral DNA and
Several studies have described the ability of Wharton's jelly cells from a variety of animal species to differentiate into neurons and/or glial cells
The precise composition of the culture medium is critical to avoid inconsistent results, especially when the reagents are obtained from different sources. With regard to RA, most of the neural induction methods in rodent or human MSCs employ a combination of RA with other chemical agents, cytokines, and growth factors
The neurotropic RNA and DNA viruses that produce CNS diseases in humans and animals use a variety of pathological mechanisms. For members of the α-
Because of this difficulty, suitable
Both herpes simplex virus 1 (HSV-1) and BoHV-1/5 establish lifelong latent infections in rabbit and cow sensory nerve ganglia
However, the molecular mechanisms by which productive and persistent viruses produce nervous system diseases are diverse and not completely understood. The results obtained in this investigation open the door to questions about neuronal death/survival mechanisms following BoHV-5 infection and replication.
This report provides the first characterization of neuron-like cells derived from bWJ-MSCs. We have demonstrated that these cells are susceptible to BoHV-5 infection. These results open the door to replace animal models to study particular aspects of BoHV-5 biology and pathogenesis. Our
Bovine Wharton's jelly-derived multipotent mesenchymal stromal cells (bWJ-MSCs)
The bWJ-MSCs were isolated and characterized as previously described
Differentiation of bWJ-MSCs
The differentiation protocol was divided into three stages. In stage I, bWJ-MSCs were resuspended at 2.8 × 104 cells/mL in Neurobasal™ medium supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich®) for 24 h. Stage II consisted of replacing the culture medium with Neurobasal™ and B27 supplement (Invitrogen®). The medium was replaced after 24 h of induction; and stage III was started by replacing the old medium with Neurobasal™ with the addition of a neuro-differentiation supplement (Invitrogen®). Stage III was sustained for 15 consecutive days. The neuron-like cells were cultured both in 25-cm2flasks for the viability assay and on Lab-Tek® well chamber slides for immunostaining (Nunc™, Rochester, NY, USA). All of the media were supplemented with penicillin (100 IU/mL) and streptomycin (100 μg/mL). All cultures were incubated at 38.5°C in 5% CO2 with 95% humidity.
Immunostaining for neural cell markers
Differentiated cells at 28 days of culture were fixed with 4% paraformaldehyde for 15 min in Lab-Tek® chamber slides (Nunc™). The cells were permeabilized for 10 min at room temperature in 0.4% Triton X-100 diluted in phosphate buffered solution (PBS). The fixed neuron-like cells were incubated overnight at 4°C with each of the primary antibodies anti-Nestin (cat # N5413, diluted at 1:100), anti-NT3 (neurotrophin 3; cat # SAB3300030, 1:50), anti-CXCR4 (chemokine receptor 4; cat # C3116, 1:20), anti-GFAP (cat # WH0002670M1, 1:50), anti-MAP2 (microtubule associated protein 2; cat # M1406, 1:20), anti-Tau (cat # T6402, 1:200), anti-ubiquitin (cat # GW10073F, 1:200), anti-β tubulin III (neuronal TuJI, cat # T8578, 1:100), and anti-N200 (neurofilament200k; cat # N4142, 1:200), all purchased from Sigma-Aldrich®. On the next day, after three washes, cells were incubated with the respective goat secondary antibody (1:100) anti-mouse, anti-rabbit and anti-chicken FITC (Sigma-Aldrich®). For nuclear staining, DAPI (1 mg/ml) was diluted in Fluormount™ aqueous medium and loaded onto samples for 15 min. The images were collected under an AxioImager® A.1 light and an ultraviolet (UV) microscope connected to an AxioCam®MRc (Carl Zeiss, Oberkochen, Germany). The images were processed using AxioVision® 4.8 software (Carl Zeiss) for each antigen, whereas values were determined by visually counting positive cells (−, 0%; +, 0-25%; ++, 25-50%; +++, 50-75%; ++++, 75-100%). The undifferentiated cells underwent the same staining procedure to assess for cross reactions among antibodies. The negative controls consisted of incubation of slides with bovine IgG and IgM isotypes as the primary antibodies.
Flow cytometric analysis for neural cell markers
Following differentiation at 28 days of culture, 1 × 106 differentiated cells were harvested after being detached with 0.25% trypsin (Sigma-Aldrich®), washed with PBS, permeabilized in 4% of paraformaldehyde, and incubated for 18 h at 4°C with the monoclonal antibodies at the same dilutions described for immunostaining in 1% Triton X-100 and 0.5% bovine serum albumin (BSA). After incubation with the primary antibodies, the cells were washed three times with PBS plus 0.1% Triton X-100. Next, a 1:50 dilution of the secondary antibody was added to 100 μl of the cell suspension and then incubated at 37°C for 30 min. The cell suspension was washed as previously described. After the final wash, the cells were fixed with 4% paraformaldehyde. Data were acquired with an Attune™ acoustic focusing cytometer system (Applied Biosystems™, Foster City, CA, USA) and at least 50,000 events were counted. The negative pattern was examined by applying the same cell suspension with the initial incubation with bovine IgG and IgM isotypes, and the result was included in the global compensation to exclude auto-fluorescence. A BL1-A (488 nm) filter was used in each analysis.
Reverse transcription-polymerase chain reaction
Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to evaluate the differentiation of bWJ-MSCs into neuron-like cells. Briefly, total RNA was extracted from the cell culture at 28 days using the TRizol™ method (Invitrogen®). mRNA expression of the nestin, N200, β-tubulin III (TuJI), GFAP and NT3 genes was reverse transcribed using an enhanced avian RT first strand synthesis kit (STR-1, Sigma-Aldrich). The PCR reaction was conducted following JumpStart™ Taq ready mix according to manufacturer's instructions (Sigma-Aldrich®) with the specific primers described in Table
Susceptibility of neuron-like cells to viral infection
After differentiation, neuron-like cells were maintained in a Neurobasal™ medium (Invitrogen™) plus B27 without FBS. The BoHV-5 strain used in the experiment was isolated from an outbreak of meningoencephalitis
Viability assay and viral growth kinetics
The cell proliferation analysis was performed using an In Vitro Toxicology Assay® Kit, an MTT-based assay, in both infected and non-infected neuron-like cells at 0, 24, 48, 72, 96 and 120 h post-infection (p.i.) (TOXI-1 Kit; Sigma-Aldrich®). For each p.i. time point, the culture supernatant was removed and 2 mL of MTT (tetrazolium salts) were added following the manufacturer's recommendations (Sigma-Aldrich®). Absorbance was measured at 600 nm with a Biophotometer (Eppendorf®, Hamburg, Germany). All reported values are means of triplicated samples.
One-step growth curve of the BoHV-5 in neuron-like cells was assayed in a multiplication kinetics experiment, followed by infection at 80% of monolayer confluence with m.o.i of 1. After 90 min of adsorption at 38.5°C, the inoculum was removed and fresh medium was added. The neuron-like cells were incubated at different time intervals (24, 48, 72, 96 and 120 h p.i). After incubation, both supernatant and lysate cells were harvested and assayed for the presence of the virus by infecting (Madin-Darby bovine kidney cells) MDBK cells as described previously
Viral nucleic acid detection by in situ hybridization assay
To estimate the number of neurons actively replicating viral DNA, neuron-like cells grown on Lab-Tek® (Nunc™) chamber slides were infected with at least 1 TCID50 per cell, incubated for 24, 48, 72, 96 and 120 h p.i. and fixed with 95% ethanol-glacial acetic acid (3:1) for 30 min in an ice bath.
Briefly, for ISH assay the infected and uninfected neuron-like cells were placed on glass slides (EasyPath®), pre-treated with pure poly-L-lysine (Sigma-Aldrich®) and fixed with 4% (w/v) paraformaldehyde (Sigma-Aldrich®) in phosphate-buffered saline (PBS) for 24 h at 4°C. Slides were treated with proteinase
The ISH positive reaction was visualized after the slides were covered using Fluormount® (Sigma-Aldrich®) medium and observed under a UV microscope. The images were collected under an Axio Imager A.1 light microscope connected to an AxioCamMRc (Carl Zeiss Oberkochen, Germany), and the micrographs were processed with AxioVision 4.7 software (Carl Zeiss). The results were expressed as the mean percentage of neurons positively marked.
Total RNA isolation and quantitative real time polymerase chain reaction (qPCR)
Upon harvesting at 24, 48, 72, 96 and 120 h p.i., the cells, monolayers and respective supernatants, and total RNA was extracted using the Trizol LS™ protocol according to manufacturer's instructions (Invitrogen®). An average of 150 ng of total RNA was used for first-strand cDNA synthesis with Enhanced Avian RT First Strand Synthesis (Sigma-Aldrich®). The qPCR was carried out and analyzed by the software on a StepOnePlus® real time instrument (Applied Biosystems™). The real time PCR mixtures (50 μl) contained 1.2 μg of cDNA, 400 nM primers and 200 nM probes. The probes were FAM-label customized for glyco-C gene sequences according to a previous report
Statistical analysis
All of the experiments were performed in triplicate or greater. Results of representative experiments are presented. Descriptive statistics include the mean ± standard deviation (s.d.). Statistical analysis was performed using ANOVA to evaluate the results. Student's
Competing interests
The authors declare that they have no competing interest.
Authors' contributions
TCC, JBN and TFA designed and directed studies, and were involved in the interpretation of the data. MCF, CSF and HFF participated in immunofluorescence assay. RG and HFF performed cytometry analysis. EFF helped in the manuscript design and discussion of results. All authors read and approved the final manuscript.
This work was supported by FAPESP (Grants 2009/17635-3 and 2010/50782-7). The authors are grateful for the donation of the bovine umbilical cords. TC Cardoso and EF Flores are recipients of funding from the CNPq (Brazilian Council for Research).
Matrix cells from Wharton's jelly from neurons and glia
Isolation, in vitro culture and characterization of foetal umbilical cord stem cells at birth
Fetal adnexa derived stem cells from domestic animal: progress and perspectives
Embryonic stem cell differentiation models: cardiogenesis, myogenesis, epithelial and vascular smooth muscle differentiation in vitro
Concise review: Wharton's Jelly-derived cells are primitive stromal cell population
Pre-activation of retinoic signaling facilitates neuronal differentiation of mesenchymal stem cells
Role of human umbilical cord mesenchymal progenitors conditioned media in neuronal/glial cell densities, viability, and proliferation
Neural progenitors, neurons and oligodendrocytes from human umbilical cord blood cells in a serum-free, feeder-free cell culture
Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro
Varicella-zoster virus infection of differentiated human neural stem cells
Differentiated neuroblastoma cells provide a highly efficient model for studies of productive varicella-zooster virus infection of neuronal cells
Isolation, proliferation, cytogenetic, and molecular characterization and in vitro differentiation potency of canine stem cells from foetal adnexa: a comparative study of amniotic fluid, amnion, and umbilical cord matrix
Isolation and characterization of wharton's jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system
The family Herpesviridae: a brief introduction
Genome of Bovine Herpesvirus 5
Bovine herpesvirus 5 (BHV-5) US9 is essential for BHV-5 neuropathogenesis
Susceptibility of sensory neurons to apoptosis following infection by bovine herpesvirus type 1
Distribution of Bovine Herpesvirus type 5 DNA in the central nervous systems of latently experimentally infected calves
A simple method of infecting rabbits with bovine herpesvirus 1 and 5
Immunohistochemical approach to the pathogenesis of clinical cases of Bovine Herpesvirus type 5 infections
A novel in situ polymerase chain reaction hybridization assay for the direct detection of bovine herpesvirus type 5 in formalin-fixed, paraffin-embedded tissues
Detection of bovine Herpesvirus type 5 in formalin-fixed, paraffin-embedded bovine brain by PCR: a useful adjunct to conventional tissue-based diagnostic test of bovine encephalitis
Comparative pathogenesis of acute and latent infections of calves with bovine herpesvirus types 1 and 5
Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains
In vitro replication of bovine herpesvirus types 1 and 5
Immediate early gene expression and gene mapping comparison among isolates of bovine herpesvirus 1 and 5
Primary infection, latency, and reactivation of Bovine Herpesvirus type 5 in bovine nervous system
Characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment
Distribution of bovine herpesvirus type 5 in the central nervous systems of latently, experimentally infected calves
Effects of bovine Herpesvirus type 5 on development of in vitro produced bovine embryos
Apoptotic and developmental effects of bovine Herpesvirus type-5 infection on in vitro-produced bovine embryos
Molecular and antigenic characterization of Brazilian bovine herpesvirus type 1 isolates recovered from the brain of cattle with neurological disease
Detection and differentiation of bovine herpesvirus 1 and 5 using multiplex real-time polymerase chain reaction


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John Holmes - The Lost Tapes
Duration: 84min 56sec Views: 30 774 Submitted: 2 years ago Submitted by:
Description: The legend returns. The long awaited missing films of John Holmes from the golden age of adult entertainment.The one and only John Holmes returns in this long awaited collection of classic erotic. Adam and eve is proud to present the King, "John Holmes" in an explosive look at some of the greatest adult entertainment ever filmed.Â
Categories: Facial


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|Publication number
|Publication type
|Application number
|Publication date
|Jul 21, 1998
|Filing date
|Sep 30, 1996
|Priority date
|Sep 30, 1996
|Fee status
|Publication number
|Paul J. Weber, Michael R. Weber, Robert Weber
|Original Assignee
|Weber; Paul J., Weber; Michael R., Weber; Robert
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (7), Referenced by (58), Classifications (14), Legal Events (3)
|External Links: USPTO, USPTO Assignment, Espacenet
1. Field Of The Invention
The present invention is directed to cutting and decorating fingernails with laser radiation. More particularly, there is provided a process utilizing laser radiation to provide an aesthetic appearance to nails.
2. Description Of The Prior Art
The application of lasers for therapeutic treatment of a variety of diseases and conditions is well known. Lasers have been used successfully for hemostasis, photodynamic destruction of some forms of tumors, removal of epidermal growths and abnormalities, and the like. However, no prior art discloses cutting fingernails with laser radiation. Generally, mechanical devices such as clippers and scissors are used for this procedure.
Furthermore, additional steps of filing and brushing may be necessary to accomplish the desired result. In some cases these operations and the use of these implements are difficult for persons which are disabled or may be arthritic. In commercial establishments, these operations are time consuming, tie-up operators limit the number of clients which can be attended which reduces profits.
In the elderly, the fingernails become ridged or lined. It is difficult in such cases to manicure or shape the nails with an emery board and/or sanding wheels. It is further desirable in such cases to soften the nails so as to work with them.
Therefore, it can be appreciated that there exists a continuing need for a new and improved simple and economical process for decorating and/or cutting fingernails which can be used commercially, as well as, for home use to obtain similar results. In this regard, the present invention substantially fulfills this need.
Washable tattoos are commercially sold which are applied to both toenails and fingernails. However, such tattoos are not useful or are lost at the beach or swimming pools.
It is understood that the term "fingernails" also relates to toe nails.
The present invention comprises a method for decorating and/or cutting or shaping fingernails with high energy laser radiation. The preferred method involves using a laser directing means which preferably include an articulated arm assembly comprising: an elongated tubular housing constructed to contain a radiation delivery end and enclosing a chamber which is inadjacent to the radiation delivery end; radiation conducting means disposed in the tubular housing for conducting laser radiation from a laser generating source toward the radiation delivery end; and means forming a gas flow path in the chamber for guiding a flow of cooling gas through the chamber in direction toward the radiation delivery end. Further, in a most preferred embodiment the human hand is secured in a template to immobilize the hand so that the laser cutting trims or decorates the fingernails more closely and evenly. The cooling agent can be generated from liquified nitrogen or solidified carbon dioxide.
More specifically, one preferred embodiment involves a method which comprises the steps of:
a) positioning a laser directing means including an articulated arm assembly containing a laser means having a laser generating source in communication with a radiation delivery means at the fingernail site; and
b) energizing a laser beam of sufficient intensity to produce the desired cutting procedure and optionally, to simultaneously provide cooling means to said laser treatment site.
In the decorating procedure, one or more coatings of a colorant may be utilized to provide a colored design with the laser. Alternatively, a design can be imparted on the nail and then a suitable colorant utilized.
In accordance with another embodiment of the present invention the cooling means may be dispensed directly to the fingernail site and not through the radiation delivery means. Alternatively, the cooling means may be passed through both the laser delivery means and dispensed directly to the laser treatment site.
It is a primary object to provide a method for decorating and cutting fingernails using laser radiation at higher power levels.
Another object of the invention is to facilitate the efficient delivery of laser radiation to the fingernail site.
It is a further object to provide a means for cutting and/or shaping fingernails and/or toenails of patients.
It is another object to provide tattoo like decorations to the nails.
Still another object of the invention is to provide a method of cutting and/or decorating or shaping fingernails which increases the speed, efficiency and safety while providing a more precise and even fingernail shape or cut.
Other objects and many of the attendant advantages of this invention will be readily appreciated as the same become better understood by reference to the following detailed description when considered in conjunction with the accompanying drawings.
FIG. 1 is a partial perspective view showing laser means cutting a fingernail wherein the cooling step is accomplished by passing a coolant through the laser cutting tip with the finger placed in accordance with the present invention.
FIG. 2 is a partial perspective view showing laser means cutting a fingernail wherein the cooling step is accomplished by passing a coolant to a plate in contact with the skin with the finger secured in a template in accordance with the present invention.
The present invention generally comprises a method for laser decorating and/or cutting fingernails. Referring to FIG. 1, a laser directing means 20 includes an articulated arm assembly which comprises a first tube 21, a rotatable elbow 22, a second tube 23, and a rotatable tube 24 which is in communication with laser cutting tip 25. Each of the tube components are preferably made of resilient stainless steel and are joined by welds to form a laser delivery system. The rotatable tube 24 may have a soft polymer cover to make more comfortable for hand adjustment. The proximal end of the laser directing means 20 is designed to receive the laser beam from a laser generator 35 controlled by a microprocessor 40. The distal end of the laser directing means 20 contains a laser cutting tip 25, which is to be positioned to administer laser radiation to the fingernail to be targeted. Further, in accordance with one embodiment of the present invention as shown in FIG. 1 a coolant fluid is supplied to the laser tip 25. Generally, the coolant fluid is an inert gas. Liquids can also be used but require removal and disposal from the cutting site. Suitable coolants include inert gases such as nitrogen, argon, carbon dioxide, and air. Inert gases are preferred since they do not pose a health hazard and are easily dissipated. Liquid nitrogen is the preferred coolant. A coolant storage tank 30 is connected through a gutted timed valve 29 and fluid conduit 28 to coolant inlet connector 26 thence to the laser cutting tip 25.
The present invention provides a method for cutting or scoring hard tissue such as fingernails comprising a laser beam provided with means enabling radiation to be emitted in form of pulsed or non-pulsed energy in sufficient number to deliver a total amount of energy that causes the cutting or scoring of the fingernails. The method being characterized in that it includes steps for adjusting the number and duration of the laser pulses in phase lag sequence of pulses of coolant to maintain a comfort level to the fingernail area, as well as, providing a safeguard to prevent overheating the laser cutting tip 25 and the delivery system if optical fibers are used. The energy level of the laser is adjusted according to cutting or decorating the fingernail.
In the cutting procedure, the laser can trim and/or shape the nails to be scalloped, rounded, etc. either by the skill of the operator or through a computer programmed mechanism (not shown).
The nails can be coated using one or more nail lacquers or pigments and then provided with a desirable decoration to one or more nails. The amount of laser energy can be adjusted so that only the coating layers and the nail itself are scored by the laser beam. A computerized and mechanized means can be employed to provide more complex designs. Commercial nitrocellulose containing nail lacquers, silver or gold flake inks, pearlite coatings, and the like are suitable coatings for decorating purposes.
FIG. 2 illustrates a preferred embodiment in a partial perspective view of a hand template known in the prior art and generally designated by reference numeral 10. In the broadest terms, the template 10 includes a lower template 12 and an upper template 14. The lower template 12 is configured to accept placement of a persons entire hand, although only a single finger is shown. The lower template 12 may be positioned in a horizontal plane. It is preferably fabricated of solid construction such as metal or plastics. The lower template 12 preferably has a representation of a human hand formed thereon by printing or painting and includes a plurality of positioning or alignment notches 16 and 18. An upper template 14 is also provided and may be positioned in a horizontal plane and configured to follow the top of a human hand. A person's hand is interposed between the top and bottom templates. The upper template 14 is further provided with a plurality of positioning or alignment notches 16 which are to be aligned with similar notches 18 on the lower template 12. In addition the top template 14 contain a plurality of openings 15 which correspond to the fingernail positions of the hand placed on the lower template 12. Openings 15 may be available in a variety of sizes.
In practice, a person places a hand to correspond to the position of the printed hand on the lower template 12. An upper template 14 having the required sizes of mail openings 15 is then positioned over the top of the hand lined up with the positioning or alignment 16 and 18 exposing the fingernail intended to be cut. The laser directing means 20 is also aligned with the specific fingernail to be treated. Standard precautions for the operator is taken such as wearing eye protection.
The laser means when energized is guided by hand gripping the rotatable tube 24 is drawn across and cuts the fingernail closely and evenly. The cut is so precise that filing or polishing is not required. The laser generator 35 provides a high energy laser beam with a power of about 1-5 watts pulsed at intervals of 0.5-2.0 seconds controlled by microprocessor 40 or non-pulsed. Likewise, the microprocessor monitors the temperature of the laser cutting tip 25, as well as, the temperature of the fingernail site. Based on this input data the microprocessor controls the gated time valve 29 to pulse the release of nitrogen gas generally 0.5 seconds after the laser beam pulse. Thus, these automatic controls make the fingernail site conformable and protect the equipment from overheating. The sequence is repeated for each fingernail. The cutting can be contained within the designated areas by having the microprocessor shut off the laser when comparison indicates that the wrong areas are about to be cut.
Suitable laser crystals include those capable of emitting laser radiation at about 1060 nm (nanometers) or 1320 nm, in particular, by using nedynium YAG (Nd-YAG) crystal or an erbium YAG (Er-YAG) crystal or a Holmium-YAG crystal.
In a further embodiment of the invention as shown in FIG. 3, the coolant may be dispensed directly to the fingernail site through coolant inlet plate 32 placed under the upper template 14. The amount of coolant provided the cutting site is controlled by microprocessor 40. In this embodiment, the cutting tip 27 is not incorporated in the cooling system. Overheating in this case is prevented in the laser generating system in which the microprocessor increases the duration between pulses.
In yet another embodiment of this invention, both the direct application of coolant, as well as, direct cooling of the laser cutting tip 25 can be integrated and controlled by microprocessor 40.
The invention herein will be illustrated by the following examples, but is not limited thereto.
An elderly person having ridged and lined nails had his fingernails manicured by the procedure of the invention. The nails were first brushed and then soaked in water for one minute to provide moisture to the nails before using the laser. The water helps to control the perpetration of the laser and prevent deep passage of heat. The hand was then immobilized on a template. A Sharplan 40� C. carbon dioxide laser was used to ablate the nail ridges. The nails were trimmed using continuous non-pulsed energy at a 1 watt level using a 60 mm handpiece which was slightly defocused and had a passage at about 4 mm per second. The nails did not require use of an emery board.
The operation of the laser was according to ANSI 2136 series of laser safety standards of the Laser Institute of America, which is incorporated herein by reference.
A laser manicure was performed so that the nails had a sculpted scalloped border with miniature partial outward portions of a circle. The nails of the person being manicured were coated with a clear acrylic coating composition which was commercially available as a nail lacquer. The procedure of Example I was followed except that the nails were not soaked in water and the beam was not defocused. The scalloping was manually performed by the laser operator.
If desired, the nails may have been coated with a plurality of colored coating layers and the laser may have been used to imprint a design.
A design was placed on the fingernails of a person by first preparing a computer generated stencil. The design was enlarged and a motorized arm was used to translate the design onto a thickly lacquered fingernail which had a coating of white, red, orange and pink as the outermost layer. A Sharplan 40� C. carbon dioxide laser at a 1 watt energy level which was in a focus mode and the pulsed mode was used with an on time of 0.05 seconds and an off time of 0.5 seconds. Through different laser penetration and melt a multicolored design was obtained.
It is understood that the process of the invention may be varied by employing various known methods to apply the design or use the laser. For example, a heat responsive colorant may be used with varying wavelengths of energy, namely, a chromophore. Alternatively, a computer driven laser may be used to create a design.
While the description above refers to particular embodiments of the present invention, it will be understood that many modifications may be made without departing from the spirit thereof. The accompanying claims are intended to cover such modifications as would fall within the true scope and spirit of the present invention.
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|Dec 24, 1996
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|Altshuler Gregory B.
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|Vasily David B.
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|Feb 19, 2004
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|International Classification
|Cooperative Classification
|European Classification
|Feb 13, 2002
|Maintenance fee reminder mailed
|Jul 22, 2002
|Lapse for failure to pay maintenance fees
|Sep 17, 2002
|Expired due to failure to pay maintenance fee
Effective date: 20020721


text:
The Discovery of the Cell
Two Cell Types
Cell Theory
Introduction to the Cell
Cell Diversity
(cc) photo by Metro Centric on Flickr
(cc) photo by Franco Folini on Flickr
(cc) photo by jimmyharris on Flickr
(cc) photo by Metro Centric on Flickr
Robert Hooke (1635 - 1703)
Peeped at cork under a microscope.
Saw "a great many little boxes."
Looked like a monk's room -- called them "cells."
The smallest unit of matter that can carry out ALL life processes.
The first to observe living cells.
Anton van Leuwenhoek (1632-1723)
Hooke and van Leuwenhoek's work led to this.
All living things made of 1 or more cells.
Cells - basic unit of life:
New Cells:
only come from the reproduction of existing cells.
Cell Size
Structure determines function and function determines structure
0.000008 inch diameter to over six feet long.
Giraffe nerve cell.
Cell size is limited by surface area/volume ratio.
Structure follows function
Nerve cell, skin cell, red blood cell?
Internal Organization
Organelles - a cell structure that performs a specific cell function.
Cell Membrane - the membrane that surrounds cells and organelles.
Nucleus - organelle - the command center & home to genetic info.
The End
Organisms whose cells have membrane-bound orgenelles and a bound nucleus.
From two different kingdoms!
Organisms whose cells lack a defined nucleus and membrane bound organelles.
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Introduction to the Cell
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text:
|Ohio State
|M Jenkins 47 YD PASS FROM C Krenzel (M Nugent KICK)
|A Davis 41 YD RUN (M Allen KICK)
|M Nugent 27 YD
|M Nugent 25 YD
|J Orr 42 YD PASS FROM J Sorgi (M Allen KICK)
|B Hartsock 3 YD PASS FROM C Krenzel (TWO-POINT CONVERSION FAILED)
|Team Statistics
|Ohio State
|First downs
|44 - 178
|44 - 180
|Passing yards
|Sacked-Yards lost
|5 - 31
|2 - 11
|Return yards
|12 - 19 - 0
|11 - 19 - 1
|6 - 50.3
|5 - 35.6
|0 - 0
|2 - 1
|5 - 40
|3 - 15
|Time of Possession
|Player Statistics
Game Story
MADISON, Wisconsin (Ticker) -- Ohio State got big plays from a pair of receivers - one on offense and one on defense - to remain the only unbeaten team in the Big Ten Conference. Michael Jenkins made a spectacular 46-yard catch to set up the go-ahead score and Chris Gamble intercepted a pass in the end zone later in the fourth quarter as the fourth-ranked Buckeyes held on for a 19-14 victory over slumping Wisconsin. Ohio State (8-0, 3-0 Big Ten) trailed, 14-13, early in the final period when Jenkins outleaped cornerback B.J. Tucker and hauled in the long pass from Craig Krenzel at the Wisconsin 39. "We just felt like we had to strike with some big ones if we were going to have a chance to beat them," Buckeyes coach Jim Tressel said. "A year ago, if you remember, we had some guys open and we did not hit them. It was good to see Mike come up with a couple of big plays." Six plays later, the Badgers bit on a fake pitch and Krenzel hit wide-open tight end Ben Hartsock for a three-yard touchdown. Under pressure, Krenzel threw incomplete on the two-point conversion. "I thought the key play of the game was 3rd-and-7 in the fourth quarter. They really hadn't done much for a quarter and a half and the ball was up in the air," Wisconsin coach Barry Alvarez said. "We had two guys in position, no one made a play on the ball and they made a big completion and were able to go down and score." Backup quarterback Jim Sorgi moved Wisconsin into position to regain the lead on the next possession. But Gamble, a sophomore flanker who has been used in the secondary by coach Jim Tressel, intercepted an underthrown pass intended for Jonathan Orr and landed in the end zone. "He catches things better than most people," Tressel said of Gamble. "It does not matter if it was intended for him or if he was covering the guy. He can go up and catch the football. That was a pretty big one. He is just outrunning athletes and you wish you could play him all the time." Freshman Maurice Clarett rushed for 133 yards on 30 carries and Jenkins had five receptions for 114 yards and a touchdown for Ohio State, which hosts Penn State next week. Anthony Davis carried 25 times for 144 yards and a TD, but the Badgers (5-3, 0-3) dropped their third straight game. "No one likes to lose, yet I thought we showed improvement," Alvarez said. "I like the way my guys competed. They made a couple more plays than we did." It did not take the Buckeyes long to grab the lead. Clarett broke off a 25-yard run on the first play from scrimmage and, after Gamble ran for three yards, Krenzel and Jenkins hooked up for a 47-yard touchdown just 90 seconds into the game. Wisconsin penetrated Ohio State territory on its first possession, then tied the game on its second. After an 18-yard punt return by Jim Leonhard, Davis dashed 20 yards to the Buckeyes' 46. Brooks Bollinger had a five-yard toss to Matt Bernstein before Davis raced 41 yards to make it 7-7. Ohio State answered with a 70-yard drive and regained the lead on a 27-yard field goal by Mike Nugent. After safety Mike Doss recovered a fumble by Bollinger at the Badgers' 32, Nugent added a 25-yarder to extend the Buckeyes' lead to 13-7 early in the second quarter. Things got worse for Wisconsin as Bollinger was sacked on back-to-back plays the next time the Badgers had the ball and left with a concussion after going 3-for-3 for 21 yards. Sorgi provided an instant boost with help from Davis, who gained 26 yards on three straight carries to start Wisconsin's next drive. Three plays after scrambling 10 yards for a first down, Sorgi hit Orr for a 42-yard TD that put the Badgers ahead at halftime, 14-13. Sorgi was 7-for-15 for 137 yards with an interception while Orr caught four passes for 107 yards. Krenzel completed 12-of-19 passes for 204 yards and Clarett set a school freshman record with his sixth 100-yard rushing effort. "I thought Craig played a very steady game," Tressel said. "He stepped up and ran a couple that were important. He threw the ball, I thought fairly well. He was under a little bit of stress, protection-wise, at one stage in the second quarter." The visiting team has won the last four games in the series, which is led by Ohio State, 50-15-5.
© Copyright 2002 USA TODAY, a division of Gannett Co. Inc.


text:
ForYourArt selects the best opportunities to SEE, COLLECT, LEARN ABOUT, and SUPPORT art every day.
Artist Talk with Miriam Cabessa
Nye + Brown (Culver City)
A walk through and discussion with Miriam Cabessa on the occasion of the exhibition of her new work, open through November 24, 2012. RSVP to Andrea Feldman Falcione at firstname.lastname@example.com to attend.
Wall Works: Art of Exchange
Santa Monica Museum of Art (Santa Monica)
Opening reception for the 20th installation of Wall Works. This biannual exhibition features the works created in the Art of Exchange, a local outreach program that connects contemporary artists with students from the Santa Monica-Malibu Unified School District. Event attendees can participate in their own arts and crafts projects at the event. On view through February 3, 2013.
"What Can We Learn from Documenta 13"
18th Street Art Center (Santa Monica)
Presented by discussion series SOC(i)AL: Art + People, this event features speakers present images and explore selected Documenta projects from the summer of 2012 throughout Kassel Germany. Moderated by Pilar Tompkins Rivas, the talk features presentations from artists and curators who participated in or visited the contemporary art exhibition Documenta 13. The evening will look at projects and reflect on the relation to current social practice.
CANCELED – Cecil Taylor
REDCAT (Downtown)
This event has been postponed indefinite
Cecil Taylor, photo courtesy of REDCAT.


text:
Sierra of The Phoenix and Olive Branch has a series called "the Good, the Bad, and the Ugly" in which she will address "several aspects of evangelical-fundamentalist life that have good, bad and ugly sides." Her second segment, on homeschooling, is now complete.
Worthwhile Reads: Homeschooling
May 18, 2012 by Libby Anne Leave a Comment
Filed Under: Homeschool, Worthwhile reads Leave a Comment
Related posts from Love, Joy, Feminism:
About Libby Anne
Libby Anne grew up in a large evangelical homeschool family highly involved in the Christian Right. College turned her world upside down, and she is today an atheist, a feminist, and a progressive. She blogs about leaving religion, her experience with the Christian Patriarchy and Quiverfull movements, the detrimental effects of the "purity culture," the contradictions of conservative politics, and the importance of feminism.


text:
Black Cat Bikinis Clothing Designer
Affadasha AlexandriaModel Female Coral Springs, Florida, US
My MM URL: http://www.modelmayhem.com/affadashaalexandria
Mayhem # 2883214
About MeHello Model Mayhem :)
My Name is Affadasha "AffA" Alexandria. I am a freelance model. I am 22 years old, 5'9 & 110lbs. I LOVE Creativity and I LOVE doing things Outside of the Box! ..I LOVE coming up with concepts and watching them come to life. I am open to new ideas, energetic, hardworking and easy to work with. Modeling is my PASSION and I'M DEDICATED!
I'm willing to work with all types of photographers, make-up artists, hairstylists, and designers. Let's make ART! Inbox for rates =)
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R L M IMAGES Photographer
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text:
Product Spotlight
Featured Research
Quick Stats
You are here
Pilot Travel Centers, Aloha Petroleum Give Back
The truckstop company presented The Salvation Army with a check, while the Hawaii-based chain gave to local young golfers.
KNOXVILLE, Tenn. and HONOLULU -- The Salvation Army got some big help from Knoxville-based Pilot Travel Centers, which presented a check for $47,500 dollars at the Pilot Travel Center in Knoxville, according to VolunteerTV.com.
The money came from the sale of paper red kettles in all Pilot stores in Knoxville, throughout the holiday season.
"Our biggest problem right now is with the economy being so low we're having more people come through our social service, they need help with rent, bills and groceries, so any help we can get at this time, we'd definitely take it," said Sgt. Stan Robison with the Salvation Army Of Knoxville.
The donation will go into a general fund to be used as needed, the report stated. Pilot also helps the Salvation Army with its Angel Tree program.
In other charity news, Aloha Petroleum presented a $5,000 check to the Hawaii State Junior Golf Association (HSJGA), continuing its support for junior golf in Hawaii for the second consecutive year, according to HonoluluAdvertiser.com.
Larry Adams, Aloha Petroleum's director of sales and marketing, presented the check at the Waialae Country Club on Jan. 13, 2009.
"After our great experience with Junior Golf over the past year, we could not pass up the opportunity to continue our support for such energetic and talented youth," said Adams in the report.
Hawaii's youngest golf pro, Tadd Fujikawa, joined Adams for the presentation. "Many of us, especially Tadd, understand the importance of supporting our young golfers here in Hawaii," said Adams.
Along with the $5,000 donation, Aloha Petroleum will continue it's Save-A-$ Club Card offer benefiting the HSJGA, the report stated. New customers who sign up for the Junior Golf Affinity fuel charge card will not only get a 3-cents per gallon discount, but Aloha will also contribute an additional 3-cents per gallon to Junior Golf for every gallon the customer purchases during a 12-month period.


text:
DETROIT (AP) Two adults and two children were shot to death as they slept Thursday in a home known to police as a hub of drug activity, authorities said.
Police did not identify the victims, but relatives said they were a mother, her two children and the man she had been dating. The children were a 9-year-old girl and 5-year-old boy, their aunt said.
"I'm hurt. My little niece and nephew just got killed along with their mother," said Pandora Eppinger, 36. "They were just little, sweet kids. Why would they do that?"
Georgia Davis, 53, identified the fourth victim as her 34-year-old son, Jason Davis, who she said had been dating the female victim. "Why would someone kill those kids? They're crazy," she said.
The boy died of a single gunshot wound to the abdomen, and the other victims all were shot multiple times at close range, according to the Wayne County medical examiner's office.
Police received the call at 3:25 a.m. Thursday, Detroit Police Chief Ella Bully-Cummings said. Officers found no signs of forced entry, and the doors and windows reinforced with two-by-fours and multiple locks, the chief said.
Police in the past have responded to shots fired and numerous narcotics complaints at the house, Bully-Cummings said.
Police believe the shooter fled in the man's vehicle. A gun was not found at the scene, but Bully-Cummings said the casings reveal a high-powered weapon was used.
No arrests had been made, and Bully-Cummings urged potential witnesses to come forward.
"I'm tired of seeing our children die," she said. "We as adults have a responsibility to our children to allow them to grow up as adults in a safe environment, and we're failing them."
Copyright 2007 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
Conversation guidelines: USA TODAY welcomes your thoughts, stories and information related to this article. Please stay on topic and be respectful of others. Keep the conversation appropriate for interested readers across the map.


text: Supplement | HBS Case Collection | December 2012 Jefferson County (C): Subsequent Issuance Daniel Bergstresser and Randolph B. Cohen Jefferson County, Alabama, faces an EPA mandate requiring sewer system upgrades. How will they finance the upgrades? What consequences will follow? Keywords: local government; political process; bankruptcy; Urban development; debt management; financial planning; Urban Development; Environmental Sustainability; Infrastructure; Government and Politics; Borrowing and Debt; Alabama; Citation:Bergstresser, Daniel, and Randolph B. Cohen. "Jefferson County (C): Subsequent Issuance." Harvard Business School Supplement 213-058, December 2012. View Details CiteView DetailsPurchase Related


text:
How to Choose a Computer Book
How to Choose a Computer Book from Overstock.com. It's easy to find computer books for everything you want to do if you know what to look for.
I got a great price and shipping was fast. Easy purchase transaction....
Peter Weverka is a veteran For Dummies author who has written about a wide variety of applications. Along with two bestselling editions of Office All-in-One For Dummies, Peter has written PowerPoint All-in-One For Dummies and Microsoft Money For Dummies.
The go-to reference for the new Office Home & Student 2013
The Home & Student version of Microsoft Office targets the home and education markets, covering the four applications most used outside the workplace: Word, Excel, PowerPoint, and OneNote. The minibooks in this essential All-in-One guide include real-world examples and projects that cover the new features and capabilities of Office 2013. Straightforward advice and beneficial projects help you to learn the basics of creating a resume in Word, establishing a home budget in Excel, developing a dynamic school presentation with PowerPoint, and taking notes in OneNote.
Office Home & Student 2013 All-in-One For Dummies is an easy-to-understand guide to the essentials of Office 2013!
|COMPUTERS / Desktop Applications / Suites
|Publisher Date
|2013-05-06 00:00:00.0
|For Dummies
|Copyright Year
|9.25 in
|7.5 in
|1.5 in
|Unit weight
|2.15 lb
|Series Name
|For Dummies
|Weverka, Peter
|Country of Origin
|United States
I got a great price and shipping was fast. Easy purchase transaction.Read More
How to Choose a Computer Book from Overstock.com. It's easy to find computer books for everything you want to do if you know what to look for.
Learn about good books that will empower young girls, tweens and teens. Shop books for girls on Overstock.com.
Best Books to Give for Mother's Day from Overstock.com. If your mom is a bookworm, use these tips to find her a literary Mother's Day gift.
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Microsoft Office Home & Student 2013 All-in-One for Dummies (Paperback)
Today $21.25


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Luxurious beachfront PH that dominates the entire top floor of the exclusive low density building NOVA. Main level living from elevator, grand second floor rooftop terrace with heated private pool, kitchen, bathroom, and lounge area. Granite counters, marble floors, massive deck, beautifully furnished, water filtration system, LED lighting, LG Art Cool air conditioning, Hunter fans, recessed ceilings, storage room, underground parking. 3 levels in total, downstairs has separate access. Gated development with infinity pool, short walk to restaurants, shops and activities.
Last Updated: Friday, 10 March 2017
All information is deemed reliable but not guaranteed. The listings on this site are displayed courtesy of the IDX program of AMPI Vallarta Nayarit MLS and may not be the listings of the site owner.


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Okra High Brow Wine Picnic, Seafood Boil at Little Cleo's, And More This Weekend in Metro Phoenix
Okra Chef Cullen Campbell will pair "low brow" cuisine with fine wines this Saturday at the High Brow Wine Picnic dinner.
High Brow Wine Picnic at Okra
Saturday, October 22
Okra chef Cullen Campbell has paired up three high-class wines with some "low-brow" cuisine for a special wine picnic this Saturday. Enjoy chicken salad paired with a Dopff & Irion Cremant Rose or chicken tenders matched with a 2012 Zind Humbrecht Muscat. Then, finish up with a hot dog paired with a 2014 Marcel Lapierre Beaujolais. The lunch is from 1 to 3 p.m., and tickets cost $35 per person. Reservations are required and can be made by calling 602-296-4147. For more information, visit the Facebook event page or Okra's website.
Austrian and German Wine Class at Central Wine
Saturday, October 22
Head over to Central Wine in Phoenix to take an Austrian and German Wine class. According to Central Wine, "the large majority of German Rieslings are actually dry," and you can taste-test this for yourself on Saturday. Guests will taste six wines paired with light bites. Class is from 4 to 5 p.m., and customers receive 15 percent off wines tasted. For more information, visit Central Wine's website.
Little Cleo's
Cajun Seafood Boil at Little Cleo's
Sunday, October 23
Little Cleo's is serving up traditional Southern dishes in the desert this Sunday at its Cajun seafood boil. Diners can dig into dishes like fresh Cajun-style shrimp and crab, corn on the cob, red potatoes, and Schreiner's andouille sausage, plus cheddar biscuits and honey butter and sweet corn hush puppies with remoulade. Make reservations for the event, which goes from noon to 4 p.m., by calling 602-680-4044 or visiting OpenTable. Tickets cost $36 per person. For more information, visit Little Cleo's website.
Miracle Mile Deli
Discounted Straw Sandwich at Miracle Mile Deli
All weekend
From now though Sunday, October 23, Miracle Mile Deli in Phoenix is offering its Straw Sandwich for $10 in honor of National Kraut Sandwich Week. The meal comes with fries and a pickle and the sandwich comes loaded with hot pastrami, melted Swiss cheese, and sauerkraut on your choice of bread. For more information, visit Miracle Mile Deli's website.
Upward Projects
Upward Projects Collecting Vintage Pencils
All weekend
Got any vintage pencils lying around your home? If you do, bring them over to any of Upward Projects restaurants and receive $5 in UP bucks, which you can use as cash at the chain's restaurants. You'll get $5 UP bucks for every 15 pencils you can bring in from now until November 30. Only three UP bucks can be redeemed for a maximum of $15 per visit. Postino's is collecting these old-school writing utensils to build an art installation at the Postino Annex in Tempe. For more information, visit Postino's website.
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- Stanley Galvanised Metal Toolbox
These heavy-duty toolboxes are ideal for use on the worksite, garage or around the home. They have a centre galvanised finish, this gives the tool box a sturdy durable construction and is also rust resistant, as are the two heavy-duty metal latches. They have a removable plastic tray which is ideal for smaller tools like screwdrivers, spanners etc, or tapes and fixings that can be found at a glance. The lid incorporates a saw groove for holding pipes and timber for cutting when there is no work bench available. The lid on has a honeycomb design to give it extra strength and durability, and the soft-grip handle folds flat in to the lid when not in use.
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Try as he might, Premier Wade MacLauchlan is having a hard time controlling the agenda in P.E.I. politics.
Give him credit, he's been proactive in trying to. He has travelled across the Island seeking opinions, has announced new conflict of interest guidelines for public servants and sought out new ways to grow P.E.I.'s economy.
But, like the Charles Dickens' character Jacob Marley, he is carrying around some chains and skeletons from his party's closet. Whether that's fair or not is of no consequence when it comes to politics - it's reality.
How he deals with those skeletons, and whether the electorate can distinguish him from his party's past sins, will decide whether he is elected premier in the spring election.
The ill-fated e-gaming venture will be an election issue. Although it's unlikely anything of a criminal nature took place, it doesn't pass the smell test.
It will be Tory gold in the upcoming campaign. Anytime a microphone is turned on, all a Tory supporter has to say, in 10 seconds or less, is something like this:
"What's the deal with the front page story in Canada's national newspaper about P.E.I.'s involvement is some crazy e-gaming and gambling initiative? And, what was Treasurer Wes Sheridan doing going to a gambling convention in London, England?"
A Liberal may be able to explain the e-gaming initiative in 15 minutes, but by then the audience will be thinning, or at least the audience's attention. The damage will have been done.
Another old skeleton never far from the surface is the Provincial Nominee Program.
Alan Rankin is someone who should know about the PNP. He was the clerk of Executive Council under Robert Ghiz from 2007 to 2009. Before that, he worked in the Liberal governments of both Joe Ghiz and Catherine Callbeck. In other words, he was an insider.
In a column in the Eastern Graphic newspaper this week, he alleges abuses occurred with the PNP file in the Pat Binns PC government, and later even more so in the Robert Ghiz administration. Says Rankin: "PNP operated in the shadows, its benefits flowing only to those on a select and much guarded list."
Although PNP has many boosters who point to how it has attracted new money and people to the province — which it has — it also has an odorous side to it that doesn't pass the smell test for many in the electorate.
So, honk honk, along with e-gaming, PNP will be along for the ride in the spring election campaign.
In that election, Premier MacLauchlan will talk about the future and the province's potential. Don't blame me for the sins of my fathers, he will say. He may even join the chorus of people condemning some of the past shenanigans.
The Tories and their leader, Rob Lantz, along with the other political leaders, will also talk a great deal about the future and P.E.I.'s potential.
But while most of the rhetoric will be about the future, the key to the election's outcome will be whether the electorate enters the polling booth looking back at past sins, or looking ahead.
Gary MacDougall is managing editor of The Guardian. He can be reached by telephone at (902) 629-6039; by email at firstname.lastname@example.com; or on Twitter.com/GaryGuardian.


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A ferret is a type of mammal that can be found both domesticated and in the wild, part of the family mustelidae, and a close relative of the polecat.
Angela once told Roran to "Watch out for ferrets."
Behind the scenesEdit
- This casual statement by Angela, made after she healed Roran's wounds in "The Whipping Post," has spawned some fan attention, including a t-shirt featuring an artwork of a ferret and the phrase "Angela says... Watch out for ferrets!"


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- posté Jul 9, 2017Perfect on Honda CTX700 DCTPar Pcprntr (1)DeJust installed this center stand on my 2014 Honda CTX700D motorcycle. Very easy installation with many helpful suggestions found all over the web. THIS INSTALL IS SIMPLE AND CAN BE DONE IN 15 MINUTES...Lire la suite
- posté Feb 12, 2017Honda Main StandPar Kelison (20)DeFits the new 2016 model 400x perfectly. Possible to DIY but attaching the spring to the main stand requires the correct tools and technique. Otherwise, this can be done in 10-15 minutes.Lire la suite
- posté Jan 19, 2017Had a very good experience with this ord...Par Rachel (1)DeHad a very good experience with this order. Did not take long at all. Picked up at the post office today. My husband put it on when he got home from work. Will definitely order again if I need more pa...Lire la suite
- posté Nov 9, 2016It is easy to put on surprising though ....When you set up the Center Stand, the car body lifts with the front descending as much as 10 cm.It is still useful if there is a chain maintenance. STAND for Off-road motorcycle (Manufactured by DRC) ...Lire la suite
- posté Oct 29, 2016Good shoppingFirst of all, the texture felt a bit crappy, but it was strong and it was good.But the Length of the spring is slightly different, the force is necessary, once again,. As we have to remove the Exhaust...Lire la suite
- posté Oct 16, 2016It was OEM Product and it was able to be...Africa Twin Center Stand is not a Standard Equipment. Honda OEM is said to be Main Stand instead of Center Stand.In order to improve the maintainability, I wanted Center Stand before delivery, but I l...Lire la suite
- posté Oct 6, 2016It is convenient to haveI endured it but eventually I bought it for MaintenanceAs well written well in Net, there were some difficulties in installation, but somehow it was possibleSpring, which is said to be the number one ...Lire la suite
- posté Oct 5, 2016This center stand is installed on a Hond...Par Tom M (1)DeThis center stand is installed on a Honda CTX700N. The installation was very easy except for the attachment of the springs. This part of the installation was extremely difficult. As a minimum, ther...Lire la suite
- posté Jul 17, 2016Caballete original Honda, llegó más rápi...Par NO NAME (1)DeCaballete original Honda, llegó más rápido de lo que pensaba. Fácil y rápido de montar. Solo se necesita hacer bastante fuerza (usar cinta de tensión o palanca) para estirar los muelles.
Todo perfec...Lire la suite
- posté Jul 11, 2016It is convenient to have it after allSince it is thing that Center Stand can be installed, I bought it and installed it.Although it took a while to install, I could do it myself.After all it is very convenient with this, cleaning of Whee...Lire la suite
- posté Jun 17, 2016Nice product and fits as stated. Quick s...Par NO NAME (2)DeNice product and fits as stated. Quick shipping and excellent service. I would buy from Webike again. Quick dispatch and good supportLire la suite
- posté Jun 9, 2016I got lost as Maintenace Stand.I was worried about the weight increase, but there is no discomfort.However, I need quite a hang to wake it up.Net's video was helpful. Also, because there is no Instruction Manual, I also checked...Lire la suite
- posté Mar 10, 2016perfectly fit into my 2009 Honda CB400 R...Par abu (3)Deperfectly fit into my 2009 Honda CB400 Revo. installation took around 30 minutes (struggling to hook the two spring!!)
a must have item for other CB400 owner out there. good for washing bike and lu...Lire la suite
- posté Mar 4, 2016The centre stand/main stand was dispatch...Par Christopher (1)DeThe centre stand/main stand was dispatched soon after purchasing online. I was able to track the journey of the stand from the warehouse in Kanagawa to Tokyo then on to Melbourne. The stand arrived at...Lire la suite
- posté Mar 3, 2016Nice product and fast delivery service. ...Par Dragon (1)DeNice product and fast delivery service. But I still not install to my bike yet. I need to wait for ctx700dct from the dealer.Lire la suite
- posté Jan 11, 2016Truly OEM! Weak point can not be found.Maintenance I bought it for propensity to upgrade.○ MaterialIt is a material with a heavy feeling made of iron. Black Painting is done.Because it is OEMOption, it seems to be the same material as othe...Lire la suite


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Cheryl Humphreys
first of all
SoldAsk us a question
First Of All, Everything is Gonna Be Fine No Matter What Happens
9x43", embossed, hand-dyed paper mounted on linen in acrylic box.
"The "friendship bracelet" series is a very personal set of works," explains Humphreys.
Derived from the daily text-based communications within my life, these pieces document moments of joy, monumental conversations, steps forward and back, and the ever-so important 'I love yous' from those closest to me. These threads serve as a visual exchange, or, 'friendship bracelet' between myself and a particular individual who has impacted my life over the past few years."
The work comes with a Certification of Authenticity signed by the Co-Founder of Tappan
The work ships in 5 - 7 business days.


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Welcome to WordPress. This is your first post. Edit or delete it, then start writing!
Hi, this is a comment.
To delete a comment, just log in and view the post's comments. There you will have the option to edit or delete them.
Give someone a lead, and you feed them for a day. Show them how to get a continuous flow of leads, and you feed them for life.


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Biomineralization is the process that produces the skeletons, shells, and teeth of most animals. It is also involved in magnetic orientation, gravity detection, and the storing of ions. This book compares a diverse number of systems, including mineral deposition of invertebrates, vertebrates, algae, and microorganisms. Emphasis is placed on the systems responsible for converting ions to minerals and the mechanisms and control of mineral form.
A book attempting to provide an overview of the broad subject of biomineralization has been a long time coming... [This book is] a very welcome addition to all of the edited symposia volumes and piles of reprints through which one must continually search in order to locate this reference or check on that viewpoint. --PALEOBIOLOGY "[A] major feat of scholarship... belong[s] on the bookshelves of every scientist interested in biomineralization." --SCIENCE "This book is a must for students studying biomineralization and will be read by any nonspecialists interested in cell biology. It is a good read with a plot that gets thicker as you work through the invertebrate material such as protoctista, sponges, annelids and molluscs on to vertebrates. The book is a major achievement and is a benchmark that will take a long time to surpass." --STEPHEN MANN, University of Bath, United Kingdom
There are currently no reviews for this product. Be the first to review this product!


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Skim It!
jeudi 07 juillet 2016 06h08
About Skim It!
**Highly rated stone skimming action. Now Free!!**
4/5 'amazeballs' Know Thy App.com
Featured in AppsGoneFree. Your daily free app resource!
Skim stones and sink boats in this fun flick game from Flippin Pixels. Curl your stone around objects, chaining combos and dodging frogs in an attempt to attain the highest score and beat your friends! Sink toy ducks to earn coins and buy upgrades.
Required: 3GS, iPod Touch3Gen, iPhone 4, iPod Touch4Gen, iPad 2Gen, iPhone 4s, iPad 3Gen, iPhone 5, iPadMini1Gen, iPad4Gen. Requires iOS 4.3 or greater. Optimized for iPhone 5.
Simmilar Apps
30.11 18:42
30.11 18:34
30.11 18:25
30.11 18:18
11.11 11:57


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Meg Ryan to Narrate "How I Met Your Dad"
She is one busy woman, and now Meg Ryan's schedule is about to be come busier yet.
According to reports, the "You've Got Mail" star will serve as the narrator in the upcoming CBS show, "How I Met Your Dad."
Bob Saget filled the role in the now ended "How I Met Your Mother," which has a similar premise to "HIMYD."
CBS has yet to confirm the news. Stay linked to GossipCenter for more show news as it becomes available.


text: HOLY Amazing price for HW home overlooking a pond in a quiet cul-de-sac location! Recently finished English basement: Theater room, full bar, family room area, 4th bedroom and full bath! This home features a wonderful open floor plan, freshly finished hardwood floors, 4 graciously sized bedrooms, huge welcoming family rm w/ a fireplace, and much more! Walk to Hawthorn Woods park! Huge value for the location!


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Welcome to the Settle-Wilder Funeral Home and Cremation Service Web Site. We are a full service family owned and operated funeral home in New Smyrna Beach, Volusia County, Florida, offering traditional and contemporary burial and cremation products and services, as well as shipouts to other states and countries, and veterans services.
We have created this site to provide our families with the most up to date services available. We welcome your comments or questions, either by email at firstname.lastname@example.com, by phone at 386-428-5757, or in person. Our goal, as always, is to offer a professional, caring staff and facility when you need it most
Thank you to all the staff for your kind and gentle handling of my mom's funeral. My mom looked beautiful and peaceful. My family was treated professionally throughout the process. I would recommend you to anyone who was in need of your services.
Florida Funeral Directors Association
Independent Funeral Directors of Florida
Cremation with Care
New Smyrna Beach
Chamber of Commerce
Professional Remembrance Planner
Veterans & Family Memorial Care
We Proudly Salute our Veterans
Recent Obituaries
Directly order flowers, view and sign the condolences book, share memories and more to celebrate lives of those dearly missed.
Take a few moments to express your wishes now and help to ease the burden on your loved ones.
A collection of answers to questions that people often have when arranging for a funeral.


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More images claiming to be of the iPhone 6 have leaked online from Apple's Chinese supply chain, revealing a slimmed down design and a glowing Apple logo.
Falling in line with all other recent rumours, the images show a smartphone larger than any iPhone to date, believed to have a 4.7-inch screen. The phone has more rounded edges similar to the iPad Air
The iPhone's volume rocker, home button and mute switch are all in their usual position, however the power button has been relocated to the side of the phone where it will be easier to reach, given its larger screen.
Most interestingly from this leak is a claim that the iPhone 6's Apple logo will light up and glow to signal notifications like missed calls and unread messages. Although a popular feature on Android handsets, no iPhone has ever had a notification light, and while we're love to see one on the iPhone 6, using the Apple logo on the back of the phone seems strange, given users rarely place their phones face-down.
The iPhone 6 is expected to be announced by Apple at a press event in either September or October, with a larger 5.5-inch model due later in the year. A wearable gadget called iWatch is also widely expected to arrive alongside the new handsets, taking advantage of the Health app, new to iOS 8, which will launch to the public in the autumn.
Source: Nowhereelse


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How to Conduct a Deep Facebook Analysis
Photo of the author, Michael Patterson by Michael Patterson on June 10, 2015
A lot of effort goes into creating a unique social marketing strategy. For those who are new to the process, we recently wrote out an entire 7-Step Guide for creating your own social media marketing strategy.
While all seven steps are important for ensuring that you have a well-rounded and comprehensive plan in place, in this post we aim to unpack the seventh step—Track, Improve and Market Your Efforts—specifically as it relates to Facebook.
Below you'll learn how to conduct your very own deep Facebook analysis using Sprout Social, and how that can help create a stronger social strategy.
1. Connect Your Facebook to Sprout Social
This process requires access to Sprout Social. If you don't use Sprout Social, you can start a free trial without having to enter credit information or download software. You won't be charged at the end of your 30-day trial, so if it's not a great fit you don't need to worry.
Go to Your Dashboard
Follow this link to get to your Sprout Social dashboard. If this is your first time, this page gives you an overview of your social media data, as well as information on connected networks and team members with access to your account.
Add Your Facebook Page
You need to attach a Facebook Page to your account so that our tool can collect your data and build you a customized report. Click the Connect a Profile icon in the upper right corner of the web app and choose to Add A Facebook Page.
Note: You can add a Facebook Business Page or, if that isn't available to you, you can click Connect a Personal Profile instead.
2. Run Your Facebook Pages Report
Sprout's Facebook Pages Report presents you with all of the data you need to conduct a thorough Facebook analysis. Please keep in mind this report can only be run for Pages with 30 or more fans or followers.
- Go to the Reports tab of the platform
- Select Facebook Pages
- Select the appropriate date range
- Your report will automatically populate
You can analyze the data right on this page, or export it as either a CSV or PDF. The PDF is customized and designed, which is a good route if you'd like to print out your report and work with pen and paper.
Note: Once you've connected your Page, it may take up to 24 hours for Sprout to collect all of your data. Sprout gathers historical Facebook Insights data going all the way back to July 19, 2011.
3. Choose Your Key Facebook Performance Indicators
There are many different metrics that you can look at when it comes to Facebook, so it's important to determine which are the most important goals for your business.
We've trimmed the list down to the seven key metrics we feel indicate strong content and a successful social strategy. Choose the metrics you would like to see increased with this analysis and optimize for them using the following steps.
According to Facebook, "post reach is the number of people who have seen your post. Your post counts as reaching someone when it's shown in the News Feed." This is a metric that you'd definitely like to see increase because the more people you reach, the more potential customers there are seeing your posts.
The algorithm that Facebook uses to decide what to show in each user's News Feed is called Edgerank, and it's made up of a handful of different factors. We list a lot of those factors in our Facebook Edgerank guide, but to keep things simple let's assume that your posts will gain more reach with an increase in clicks, shares, comments and likes.
Someone sharing one of your Facebook posts is a strong indicator that your post resonated with them, and they think that their friends and followers will enjoy it as well. When it comes to sharing, users can:
- Share on their own Timeline
- Share on a friend's Timeline
- Share on a Page they manage
- Share in a private message
- Share in a group
No matter how your fans share, this fuels the Edgerank score for that piece of content and, in turn, increases that post's reach.
Comments allow you to get direct feedback on your content from your audience. It may not always be positive, but negative comments may be an indicator that you should stop and re-think your content. Positive comments and engagement indicate that your content is resonating with your audience. In the end, more comments is also more fuel for the Edgerank algorithm, so it's important to monitor closely and understand when sentiment is positive and when things are turning negative.
As your content starts to draw engagement, it will naturally start to see more reach. Then as long as your click-through rates remain constant, you'll see an increase in the overall clicks on your posts or clicks to your website.
This is the number of people who click the "Like" button on one of your posts. Facebook likes are a clear indicator that somebody is enjoying your social media content. This is a metric that you should work on increasing, as it shows people are favoring your posts.
Engaged Users
The number of people who clicked anywhere in your post without generating a story plus the number of unique people who created a story about your Page post. This is a great metric to increase because it means people are creating their own unique stories about your posts.
The number of people who have created a story from your post. Stories are created when someone likes, comments or shares your post, as well as when someone answers a question you posted or responds to an event invite.
4. Ask Yourself the Right Questions
There are five very important questions that you need to ask yourself when conducting your Facebook analysis. The answers to these questions will guide your future strategy and make you a more effective Facebook marketer.
What Kind of Messaging Resonates?
A major reason to conduct a Facebook analysis is to find out which of your content performs the best; then, use that knowledge to guide your copywriting strategy.
Halfway down your Facebook Page report you'll see a section titled Content Breakdown. This provides you with information on how each individual post you've created has performed.
Click your key performance metric at the top to sort by the top performing pieces of content. Study these posts to see what they all had in common.
Did you ask your audience for their opinion on a matter? Was it less sales driven than others? Was it funny? Figure out what about that post had an impact on your audience and optimize future copy for that.
What Type of Posts Get Shared Most?
Facebook posts are typically broken down into four categories, and your engagement rate will vary depending on which type your audience prefers.
- Statuses
- Photos
- Links
- Videos
Find the part of your report labeled Your Content, and focus on the By Story Type section.
Although it's important for you to take advantage of all four post types, you should also favor the style that your audience engages with. This leads to increased overall engagement.
Who Are Your Facebook Users?
Understanding the demographic breakdown of your Facebook audience is a powerful insight that could help inform which types of products or content you post about and how you position them.
Locate the Sharing portion of your report and study the Sharing by Age & Gender section.
Do some additional research to find out how you can talk to this audience, and use that to inform your future publishing strategy.
Tip: Couple your personal demographic information with all social network demographic information to figure out what other networks you could benefit from.
Where Are Your Facebook Users?
As companies and social media sites go global, the idea of geo-targeting has become very popular. Distributing different content to members of their Facebook audience in different locations is something that great Facebook marketers should always be considering.
In the same Sharing section that you found your demographic information, locate the Sharing by Location.
Figure out where in the world your customers are and target those locations with more relevant content. You could also try to target the locations that you don't see in order to build more brand awareness in areas that lack it.
When Should You Post to Facebook?
Most social media marketers are fascinated with finding the best day for them to post to Facebook. According to Google, May 2015 saw the highest amount of people ever searching for the term "best day to post to Facebook."
That's why our final step is to analyze your Sharing by Day of Week stats in the Sharing section.
If you have a limited amount of content, or don't like posting every day of the week, use this information to pick and choose the best days to get your audience to share your posts.
It's All About Your Followers
All these strategies are just a way for you to have a more meaningful connection with your social following, so that you can create lasting social relationships. If done well, and consistently improved upon, you should see an increase in the amount of traffic, leads and revenue you drive from social media.
Learn more about Sprout Social's Facebook management and Facebook analytics features here.
Michael Patterson: Michael enjoys writing about all things social media, and his insights can be found on Adweek's SocialTimes, Social Media Today and Social Media Examiner. When he isn't pouring over blog posts he can usually be found exploring Chicago's unique neighborhoods and breweries.


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New York, New York – (The Hosting News) – August 29, 2008 – Hosting reseller and multiple domain web hosting provider, Cirtex Hosting, has launched its value-added, exclusive feature, designed to allow video sharing scripts to web sites, for shared hosting and Virtual Dedicated Servers.
John Xie, President of Cirtex Corp. noted, "For the past several years, we have seen the real value and growth in online video and we have made a series of moves to help secure our spot as a leader in the video hosting market. With today's launch of Video Script Installer we continue our commitment to offering our customers the tools they need to succeed and compete as the web continues to evolve. VSI enables our customers to setup a fully-functional, automated video sharing portal in less than three minutes."
The Video Script Installer (VSI) will be offered to customers on its two flagship hosting brands, CirtexHosting (www.cirtexhosting.com) and HostV (www.hostv.com).
The Video Script Installer, exclusively available from CirtexHosting, is accessible through an intuitive interface within each client's web site control panel. The interface allows one-click installation of ClipBucket, VidiScript, and PHPmotion. Each of the scripts allows hosting users to build Web sites with similar usability as Google's popular video sharing site, YouTube. Cirtex Corp. is also making available RED5 and FFMPEG – server side video processing engines – to power the three VSI scripts.
The company recently forged exclusive partnerships with both VidiScript and ClipBucket to offer their scripts to Web hosting customers. Cirtex Corp. clients are able to take advantage of VSI as a value-added feature at no additional charge.
Founded in 2003, Cirtex Hosting is a provider of shared and reseller multiple domain Linux web hosting solutions, offering 24/7 technical support, 99.5% guaranteed uptime, and a 30-Day unconditional guarantee, serving hundreds of small to medium sized businesses internationally. The company serves thousands of customers internationally through three brands – CirtexHosting, MySitesOnline, and HostV.
To learn more about HostV, a provider of Virtual Dedicated Servers and Managed Dedicated Hosting, please visit: www.hostv.com.
For more about CirtexHosting, a provider of Shared and Reseller Hosting, please visit: www.cirtexhosting.com.