text:
Carrot Drop Cookies
Recipe by katia
I found in jewishfood-list.com and they are very tasty. I didn't make the frosting but the original recipe calls for it.
Top Review by Lynne the Pirate Qu
FANTASTIC! I was pressed for time, so I took reviewer Color Caurd Mom's suggestion and made this into bar cookies (10"x15" pan, 20 minutes). They came out perfect! I haven't frosted them yet, just been diving into the plain bars... I can only imagine how heavenly they'll be when I get around to making the frosting, though they don't really need it. Next time, I may use a bit less sugar as they were a little sweet for my taste, but other than that I wouldn't change a thing. Thanks for sharing!!
See Discussion:3 Reviews
Ingredients Nutrition
- 2 cups carrots, grated
- 1 cup butter
- 3⁄4 cup white sugar
- 3⁄4 cup brown sugar
- 2 eggs
- 2 teaspoons vanilla
- 2 1⁄2 cups all-purpose flour
- 1 teaspoon baking soda
- 2 teaspoons cinnamon
- 1⁄2 teaspoon salt
- 1 cup pecans, chopped
- 4 cups powdered sugar
- 1⁄2 cup butter, softened
- 8 ounces cream cheese
- 1⁄2 teaspoon vanilla
Directions See How It's Made
- Heat oven to 350°F.
- Combine the butter and the two sugars in a large mixer bowl.Beat until creamy (2 - 3 minutes).Add eggs and vanilla. Add flour, baking soda, salt and cinammon. Stir in pecans and carrots.
- Drop dough by tablespoonsfuls onto greased cookie sheets. Bake for 12 to 15 minutes. Let them cool completely.
- Meanwhile beat the powdered sugar with the butter, cream sheese and vanilla until creamy.
- Frost each cooled cookie with 2 teaspoonfuls frosting.


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Dear Friend,
The West Fork Complex Fire near Pagosa Springs has burned more than 95,000 acres and is as of this writing still out of control. The East Peak Fire near Walsenburg and La Veta has burned more than 13,500 acres. These are just two of the fires that are burning in the 3rd District right now, and it is still early in the summer. Tens of thousands of acres of forests are already gone and entire communities are being threatened.
Just like the wildfires that have ravaged Colorado and other Western States over the past decade, these fires have destroyed property, and are doing irreversible damage to the environment and watersheds on which we rely.
Brave men and women are working around-the-clock to stop the devastation. They are truly incredible, and I want to thank them for all they are doing to protect property, save lives and contain these wildfires.
The incident commanders in charge of suppression efforts on the West Fork Fire told me that the behavior of the fire has been unprecedented. Because of all of the beetle-killed timber, unnaturally dense forest and dry conditions, the fire has acted in a way that defies computer models.
The most tragic part of this is that the occurrence of these forest fires could be reduced, if not outright prevented, with commonsense healthy forest management. With millions of acres of beetle-killed timber, prolonged drought, unnaturally dense undergrowth, and warm temperatures combining to create prime conditions for fire, it's undeniable that Colorado's forests need immediate attention.
This week I was joined by my Republican colleagues from Colorado and the Chairmen of the Congressional Western Caucus in introducing a resolution to express the sense of the House of Representatives that more must be done to address the conditions of forests on federal lands.
This resolution dovetails with my Healthy Forest Management and Wildfire Prevention legislation, and is a call to action to address the critical condition of Western Forests in order to prevent future destruction. I'm working with my colleague, Natural Resources Committee Chairman Doc Hastings, to address the need for proactive forest management in addition to fixing the broken secure rural schools system, and am optimistic that we will see movement on forest legislation in the House within the summer, and take crucial steps toward addressing this emergency.
Source: http://tipton.house.gov/press-release/update-congressman-scott-tipton-western-emergency

subset: commoncrawl


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Question about HP Pavilion dv6000z Notebook
I just bought a new computer with Windows Vista Home Premium. I cannot create a backup of recovery files on a DVD-R disc. Everything starts off fine, then at the end this error comes up: The backup did not complete successfully. An error occured. The following information might help you reslove the error. The request could not be performed because of an i/o device error (0x8007045D) Please help me. Much appreciated. I have a HP dv6799 with pioneer dvr-k17b dvd writer,2gig ram,cpu intell t-9300. It originally came with Vista home premium but I upgraded it to Ultimate SP1 and now that I want to get a complete backup of my hard drive, at the end of the first DVD iget the same error (0x8007045D). I have tried the clearing of restore points,shadow copies etc. sofar nothing has worked. I hope somebody finds a soluion, surely Microsoft doesn't give a damn!!!
Posted by mgolchin13 on
Unsuccessful backup. I/O device error (0x8007045D)
I have a Gateway desktop. That is all I know about it. Wanted to back up my files but I get this same error: "Unsuccessful backup. I/O device error (0x8007045D)" Tried an different disk to no avail. I do not know what to do to solve the problem. Reading the Google answers do not help me.
I keep getting Error 0x8007045D when I try to copy certain files. I can copy other files with no problems. I have read a few forums and everybody has a different laptop (mine is a Gateway, but I've seen 4 other models/makes with similar problems). Perhaps it is Vista? All seem to be with newer model laptops...All seem to be laptops.
I am not sure why some copying of files work and others don't.
Unsuccessful backup. I/O device error (0x8007045D) please help
Try this http://support.microsoft.com/kb/952272 it will take you through your problem step by step hope it helps
Posted on Oct 25, 2008
i had the same problem with my new hp dv6000 notebook. the recovery disc creation crashes, i tried different discs but all failed, tried different ways, but all failed, so rang HP support, said them my problem, and they send me a new set of Recovery DVD in a week absolutely free.
what i came to know from hp was, sometimes the software gets corrupted. so thers no use of trying it in different ways.
so i would suggest u to geton to the website www.hp.com, ring hp support, tell them whts going wrong, and they gonna send u a fresh copy of ur system recovery programme.
hope it helped.
Posted on Oct 25, 2008
HP Pavilion DV6000 Notebook main problem's are Power Problem, Vista Error etc..
For these problems you can visit a link by just Click Here .
For any other problems with this Notebook you can Click Here
Posted on Oct 08, 2010
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Posted on Jan 02, 2017
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text:
"Every agricultural
setting is different," said Young. "Everyone has different questions and
things to be addressed. We try to touch on topics we feel could be
pertinent for the times and make ourselves available to be open to
questions and discussion throughout the day."
Thursday's meeting
was held at the Ohio State University Extension office in Van Wert and
addressed such topics as changing weed population, chicken manure's fit
into a phosphorus management plan, winter manure application rules in
the Western Lake Erie Basin, science versus sales and safety concerns
with reintroducing livestock production back to the farm.
under-riding theme in much of the discussion pertained to understanding
the importance between soil applications, the amount of phosphorus in
applications and rules pertaining to the Western Lake Erie Basin
Other topics included using livestock manure with growing corn to capture the nitrogen value from the soil.
his seminar dealing with how chicken manure fits into the nutrient
phosphorus management plan, Ed Lentz of Agriculture and Natural
Resources from Hancock County stated that the nutrient value of poultry
manure comes from the balance concerns between phosphorus and nitrogen.
hit phosphorus restrictions quicker because of water restrictions,"
said Lentz. "Manure can become a real problem when compared to water
Lentz said the benefits of poultry manure include
improved soil structure, water infiltration and the shortening of
organic matter.
Lentz noted that Van Wert County is one of the
lighter counties around as far as poultry production, especially versus
heavy poultry farming in Mercer County and increased farming in Paulding
"Poultry manure is higher in phosphorus than manures such
as dairy, beef, swine and sheep but that factor can also cause problems
in water quality," observed Lentz.
Lentz said that the
availability of manure nutrient depends on the nutrient analysis of the
manure, mineralization of the manure, application method and rate of
"Because the phosphorus level in chicken manure is
higher than other manures, it is more difficult to keep a proper
balance," said Lentz. "There needs to be a balancing of soil test,
determining crop needs, manure analysis and the proper method of
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text:
SW-126 (69 x 35)
The SW-126 is a standard sized modern style bathtubs designed with extra storage shelves. Despite its intricate design, the SW-126 bathtub is all one pieces. All of our bathtubs are made of durable white stone resin composite and available in a matte or glossy finish. This tub combines elegance, strength, and convenience with its high quality construction and chic modern design. This angular shelved designed freestanding tub will surely be the center of attention and will add a contemporary feel to your new bathroom. Its height from drain to overflow will give plenty of space for an individual to enjoy a comfortable relaxing bathtub experience.
Item#: SW-126
Item#: SW-126
Product Size (inches): 68.9 L x 35.4 W x 22.4 H inches
Material: Solid Surface/Stone Resin
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Water Capacity: 79 Gallons
Drain to Overflow: 15 Inches
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text:
Malala turns 18,opens school for Syrian girls.Malala Yousafzai, the youngest winner of the Nobel Peace Prize, celebrated her 18th birthday in Lebanon on Sunday by opening a school for Syrian refugee girls and called on world leaders to invest in "books not bullets".Malala became a symbol of defiance after she was shot on a school bus in Pakistan 2012 by the Taliban for advocating girls' rights to education. She continued campaigning and won the Nobel in 2014.
Malala turns 18,opens school for Syrian girls
"I decided to be in Lebanon because I believe that the voices of the Syrian refugees need to be heard and they have been ignored for so long," Malala told Reuters in a schoolroom decorated with drawings of butterflies.
The Malala Fund, a non-profit organisation that supports local education projects, paid for the school in the Bekaa Valley, close to the Syrian border. It can welcome up to 200 girls aged 14 to 18."Today on my first day as an adult, on behalf of the world's children, I demand of leaders we must invest in books instead of bullets," Malala said in a speech.For detail Read on the original site.


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Part of a series on Tibetan Buddhism
|Timeline · Related-topics
|Nyingma · Kagyu · Sakya · Gelug · Bön
|Key Concepts
|Three marks of existence · Skandha · Cosmology · Saṃsāra · Rebirth · Bodhisattva · Dharma · Dependent Origination · Karma
|Major Figures
|Gautama Buddha · Padmasambhava · Je Tsongkhapa · Dalai Lama · Panchen Lama · Lama · Karmapa Lama · Rinpoche · Geshe · Terton · Tulku
|Buddhahood · Avalokiteśvara · Four Stages of Enlightenment · Tantric yoga · Paramitas · Meditation · Laity
|Changzhug · Drepung · Dzogchen · Ganden · Jokhang · Kumbum · Labrang · Mindroling · Namgyal · Narthang · Nechung · Pabonka · Palcho · Ralung · Ramoche · Sakya · Sanga · Sera · Shalu · Tashilhunpo · Tsurphu · Yerpa
|Chotrul Duchen · Dajyur · Losar · Monlam · Sho Dun
|Kangyur · Tengyur · Tibetan Canon · Mahayana Sutras
|Sand mandala · Thangka · Ashtamangala · Tree of physiology
Outline · Comparative Studies · Culture · List of topics · Portal
Swayambhunath (Devnagari: स्वयम्भूनाथ स्तुप; sometimes romanized Swoyambhunath) is an ancient religious complex atop a hill in the Kathmandu Valley, west of Kathmandu city. It is also known as the Monkey Temple as there are holy monkeys living in parts of the temple in the north-west. The Tibetan name for the site means 'Sublime Trees' (Wylie:Phags.pa Shing.kun), for the many varieties of trees found on the hill. However, Shing.kun may be a corruption of the local Newari name for the complex, Singgu, meaning 'self-sprung'.[1] For the Buddhist Newars in whose mythological history and origin myth as well as day-to-day religious practice, Swayambhunath occupies a central position, it is probably the most sacred among Buddhist pilgrimage sites. For Tibetans and followers of Tibetan Buddhism, it second only to Boudhanath.
The Swayambhunath complex consists of a stupa, a variety of shrines and temples, some dating back to the Licchavi period. A Tibetan monastery, museum and library are more recent additions. The stupa has Buddha's eyes and eyebrows painted on. Between them, there is something painted which looks like the nose - but is the Nepali symbol of 'unity', in the main Nepali language dialect. There are also shops, restaurants and hostels. The site has two access points: a long stairway, claimed to have 365 steps, leading directly to the main platform of the temple, which is from the top of the hill to the east; and a car road around the hill from the south leading to the southwest entrance. The first sight on reaching the top of the stairway is the Vajra. Tsultrim Allione describes the experience:
We were breathless and sweating as we stumbled up the last steep steps and practically fell upon the biggest vajra (thunder-bolt scepter) that I have ever seen. Behind this vajra was the vast, round, white dome of the stupa, like a full solid skirt, at the top of which were two giant Buddha eyes wisely looking out over the peaceful valley which was just beginning to come alive.[2]
Much of Swayambhunath's iconography comes from the Vajrayana tradition of Newar Buddhism. However, the complex is also an important site for Buddhists of many schools, and is also revered by Hindus.
According to Swayambhu Purana, the entire valley was once filled with an enormous lake, out of which grew a lotus. The valley came to be known as Swayambhu, meaning "Self-Created." The name comes from an eternal self-existent flame (svyaṃbhu) over which a sūpa was later built.[3]
Swayambhunath is also known as the Monkey Temple as there are holy monkeys living in parts of the temple in the north-west. They are holy because Manjushree, the bodhisattva of wisdom and learning was raising the hill which the Swayambhunath Temple stands on. He was supposed to leave his hair short but he made it grow long and head lice grew. It is said that the head lice had transformed into these monkeys.
The Bodhisatva Manjushri had a vision of the lotus at Swayambhu and traveled there to worship it. Seeing that the valley can be good settlement and to make the site more accessible to human pilgrims, Manjushri cut a gorge at Chovar. The water drained out of the lake, leaving the valley in which Kathmandu now lies. The lotus was transformed into a hill and the flower become the Swayambhunath stupa.
Swayambhunath, is among the oldest religious sites in Nepal. According to the Gopālarājavaṃśāvalī Swayambhunath was founded by the great-grandfather of King Mānadeva (464-505 CE), King Vṛsadeva, about the beginning of the 5th century CE. This seems to be confirmed by a damaged stone inscription found at the site, which indicates that King Mānadeva ordered work done in 640 CE.[3]
However, Emperor Ashoka is said to have visited the site in the third century BCE and built a temple on the hill which was later destroyed. Legend has it that the Buddha himself visited Swayambhunath and gave teachings there two hundred years earlier.[4]
Although the site is considered Buddhist, the place is revered by both Buddhists and Hindus. Numerous king Hindu followers are known to have paid their homage to the temple, including Pratap Malla, the powerful king of Kathmandu, who is responsible for the construction of the eastern stairway in the 17th century.[5]
The stupa consists of a dome at the base. Above the dome, there is a cubical structure present with eyes of Buddha looking in all four directions with the word "unity" in the main Nepali dialect between them. There are pentagonal Toran present above each of the four sides with statues engraved in them. Behind and above the torana there are thirteen tiers. Above all the tiers, there is a small space above which the Gajur is present.
The dome at the base represents the entire world. When a person awakes (represented by eyes of wisdom and compassion) from the bonds of the world, the person reaches the state a bit higher. The thirteen pinnacles on the top of it symbolises that sensient beings have to go through the thirteen stages of enlightenment to reach Buddhahood.
On each of the four sides of the main stupa there are a pair of big eyes which represent Wisdom and Compassion. Above each pair of eyes is another eye, the third eye. Saying goes that when Buddha preaches, cosmic rays emanate from the third eye which acts as message to heavenly beings, so that those interested can come down to earth to listen to the Buddha. The hellish beings and beings below the human realm cannot come to earth to listen to the Buddha's teaching, however, the cosmic ray relieves their suffering when Buddha preaches.
There are carving of Panch Buddhas (five Buddhas) on each of the four sides of stupa. Apart from this, idols of the Buddhas are at the base of the stupas. Panch Buddhas are Buddha in metaforical sense in Tantrayana. They are Vairochana (occupies the center and is the master of the temple), Akshobhya (faces the east and represents the cosmic element of consciousness), Ratna Sambhava (faces the south and represents the cosmic element of sensation), Amitabha (He represents cosmic element of Sanjna (name) and always faces the West) and Amoghsiddhi (He represents the cosmic element of conformation and faces the north).
Each morning before dawn, hundreds of Buddhist (Vajrayana) and Hindu pilgrims ascend the 365 steps from eastern side that lead up the hill, passing the gilded Vajra (Tibetan: Dorje) and two lions guarding the entrance, and begin a series of clockwise circumambulations of the stupa.
See also
- ↑ Allione, Tsultrim (1986). Women of Wisdom. London: Arkana. xxxiii. ISBN 1-85063-044-5.
- ↑ Allione, Tsultrim (1986). Women of Wisdom. London: Arkana. xiv. ISBN 1-85063-044-5.
- ↑ 3.0 3.1 Shaha, Rishikesh. Ancient and Medieval Nepal. (1992), p. 122. Manohar Publications, New Delhi. ISBN 81-85425-69-8.
- ↑ Wills, Kendall J. On the Trail Of the Buddha. New York Times, March 8, 1987. Online: [1] (Accessed: May 16, 2008).
- ↑ Lonely Planet Nepal (2005). Swayambhunath.
Additional References
- Swoyambu Historical Pictorial. Edited by Richard Josephon. (1985). Satya Ho. Kathmandu.
- Psycho-cosmic Symbolism of the Buddhist Stūpa. Lama Anagarika Govinda. (1976) Dharma Books. Berkeley, California. ISBN 0-913546-35-6; ISBN 0-913546-36-4 (pbk).
Further reading
- Ehrhard, Franz-Karl (1989). "A Renovation of Svayambhunath-Stupa in the 18th Century and its History (according to Tibetan sources)." Ancient Nepal - Journal of the Department of Archaeology, Number 114, October-November 1989, pp. 1-8.
External links
|Wikimedia Commons has media related to: Swayambhunath
- Virtual tour of Swaymabhu using panaroma micromedia flash media
- Swayambhunath Eyes by Tim van Woensel
- Swayambhunath Photos, October 2007
- Images from Swayambhunath
- Swayambhunath images on flickr
- Swayambhunath at Sacred Destinations : Description of Sights and Icons
Coordinates: 27°42′54″N 85°17′24″E / 27.715°N 85.29°Eko:스와얌부나트 사원 ne:स्वयंभू ja:スワヤンブナート ru:Сваямбунатх sh:Swayambhunath


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Director Elections
Grand Valley Power's members elect their board by mail-in ballots or in person at the Annual Meeting of Members held each summer. If three or fewer members seek election, then Grand Valley Power will forego the mail ballots and the election of the Directors will take place during the annual meeting. Grand Valley Power's board members, elected at-large, serve three-year, staggered terms.
Each Grand Valley Power member has the right to vote for up to 3 directors to be elected. Members have only one ballot per election, regardless of the number of accounts with Grand Valley Power. The account holder or spouse on the Grand Valley Power account is eligible to vote.
Unlike governmental elections, not all registered voters in the household can vote in the Grand Valley Power elections. Members who are unclear of their membership or account status are urged to call Grand Valley Power's Member Services department at 970-242-0040 for verification.
Running for the Board of Directors
To be eligible to become or remain a director, a person must be a Grand Valley Power member and receive electric service from Grand Valley Power at the member's primary residence. A director cannot be engaged in a competing enterprise or in other businesses involving a conflict of interest. Grand Valley Power's bylaws provide in-depth information on director qualifications, terms, elections, meetings and officers.
Director Elections will be held on August 3, 2017 at the Member's Annual Meeting.


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$200.00 9 months ago
wrestling mats for sale! - $200 (cedar rapids)
I'm Selling one 10 foot by 10 foot wrestling mats. They are located in cedar rapids. They are in great condition. They were previously used in my wrestling room but since we got new wrestling mats we are getting rid of the old ones to make space. Not accepting any sort of trade at this time. Thanks for looking! text is the best way to reach me.319558914zero
9 months ago
Cedar Rapids, IA
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Wrestling Mats and Fitness Equipment For Sale!!!
Gym is closing. For sale is an olympic size wrestling mat, wall mats, pull up bars, gymnastics rings, smith machine, treadmills, medicine balls, slam balls, jump ropes, gymnastics tumbling mat, resistance bands, kettle bells, gym fans, etc.
11 months ago
Iowa City, IA
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World number 49 Kohlschreiber, 23, beat doubles partner Youzhny of Russia, seeded third, 2-6 6-3 6-4.
Kohlschreiber is the first German to win in Munich since Michael Stich in 1994 and he will break into the top 40 for the first time Monday.
He later teamed up with Youzhny to win the doubles.
After going 2-0 up Kohlschreiber lost six straight games as the first set slipped away, but the German broke early in the second and in game five of the decider before converting his third match point.
"It's such an amazing thing to win your first ATP title and to do it in Germany," said Kohlschreiber.
"I'm so happy! This is just a crazy day. I need a few days before this sinks in."
Youzhny, who was going for his second title of the year, said: "It was a tough match, Philipp played really well.
"It was really good for me to reach the final here. I didn't feel I was in the best shape in the beginning of the week.
"I improved for every match here in Munich, that is very important for me."


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Characterisation of the wheat (
Plant genomics
Phosphorus (P) is an essential macronutrient for plant growth and development. To modulate their P homeostasis, plants must balance P uptake, mobilisation, and partitioning to various organs. Despite the worldwide importance of wheat as a cultivated food crop, molecular mechanisms associated with phosphate (Pi) starvation in wheat remain unclear. To elucidate these mechanisms, we used RNA-Seq methods to generate transcriptome profiles of the wheat variety 'Chinese Spring' responding to 10 days of Pi starvation.
We carried out
Our transcriptome profiles demonstrated the impact of Pi starvation on global gene expression in wheat. This study revealed that enhancement of the Pi-mediated signalling cascade using
As a key component of plant cell molecules, phosphorus (P) is an essential macronutrient for plant growth. Large quantities are used in fertiliser, but worldwide P resources will be exhausted by the end of this century
Wheat is a major staple food crop in many parts of the world in terms of both cultivation area and prevalence as a food source. To meet the increasing global demand for wheat, this crop's exploitation of nutrients must be made more efficient and its requirement for nutritional fertilisers reduced. Because wheat is primarily grown on substrates with low P levels, such as the acidic soils of tropical and subtropical regions and the calcareous soils of temperate regions, an important constraint to wheat production is its lack of tolerance to −P.
Various genetic approaches have been used to understand genetic control of −P tolerance in wheat; these include aneuploid analyses of the nulli-tetrasomic series and wheat alien chromosome addition lines of the cultivar 'Chinese Spring' (CS) and quantitative trait locus (QTL) mapping
Several technologies, including massively parallel sequencing and microarray analysis, have recently been used to simultaneously catalogue the effects of −P on the expressions of thousands of genes in model species
In model species, transcriptome profiling and the quantification of gene expression levels are generally performed by mapping reads from the NGS analysis to a reference genome sequence and annotating genes. The strategies for model species are not feasible in wheat, as its reference genome sequence and gene annotation are still incomplete; an international project to achieve these goals is currently making progress (IWGSC: International Wheat Genome Sequencing Consortium,
The database of putative full-length cDNAs for wheat, TriFLDB, has released approximately 16,000 full-length cDNAs (
Although computer-based
In this study, we verified the
Results and discussion
Construction of the wheat transcript dataset
A set of transcripts consisting of cDNAs from TriFLDB and contigs from RNA-Seq reads was constructed as shown in Figure
Additional file 1
Comparison of the
Click here for file
Additional file 2
Statistical analysis of the non-redundant set of wheat transcripts obtained from three assembly programs.
Click here for file
Figure 1
Strategy used in the wheat RNA-Seq analysis
Strategy used in the wheat RNA-Seq analysis. A set of 29,617 wheat transcripts was constructed from 15,047 non-redundant contigs and 14,570 full-length cDNAs in TriFLDB. To identify the responsive transcripts under –P conditions, all reads were aligned to the transcripts using Bowtie, and a G-test with an FDR value cut-off < 0.01 was used.
Contig quantity and mean length are often used in
Additional file 3
Assessment of contigs aligned to full-length wheat cDNAs in TriFLDB. All quality-controlled reads were aligned to these full-length cDNAs using Bowtie and three assembly programs. The proportion of numbers and bases of the full-length transcripts covered by each assembly program were calculated.
Click here for file
Because the 67,616 non-redundant contigs could indicate homology to either plants or non-plants, we treated them as contaminants and removed them from the analysis, except when a BLASTX hit matched a land plant. The contigs were analysed for similarity/sequence conservation against nr (non-redundancy datasets of various species) using a BLASTX search (E-value ≤1E-03). A total of 15,047 contigs were found in land plants (top hit), with 91.4% in monocots, such as
Additional file 4
Conservation of sequences between wheat transcripts and other land plants. The number of contigs showing significant similarities (E-value <1E-03) when compared with nr according to BLASTX is shown.
Click here for file
Mapping reads to the wheat transcript dataset
Characterisations of contigs and cDNAs from the 29,617 transcripts are presented in Table
Distribution of RPKM values was assessed by comparing contigs and cDNAs (Additional file
Additional file 5
RPKM values of contigs and cDNAs in TriFLDB. (PDF 77 kb)
Click here for file
Identification of differentially-expressed wheat transcripts under −P
A G-test (FDR [False Discovery Rate] <0.01) of the RPKM-derived read counts was performed to detect differences in gene expression between control and –P-treated plants, and to identify responsive wheat transcripts under –P conditions (step 7 in Figure
Figure 2
Distribution of transcripts upregulated or downregulated in response to −P
Distribution of transcripts upregulated or downregulated in response to −P. The total number of upregulated or downregulated transcripts identified by RNA-Seq was determined in roots and shoots under –P conditions. Each bar shows the distribution of transcripts with their matching cDNAs in TriFLDB (light grey) and contigs (grey).
Functional annotation of responsive transcripts under –P
To obtain a functional annotation of upregulated wheat transcripts under –P conditions, we used Gene Ontology (GO) biological process categories. GO annotations were assigned to 40.7% (409) of the root transcripts and 34.4% (974) of the shoot transcripts. We identified the top 20 GO categories into which upregulated transcripts in roots and shoots were distributed (Figure
Figure 3
Distribution of Gene Ontology (GO) biological process categories for upregulated contigs
Distribution of Gene Ontology (GO) biological process categories for upregulated contigs. The number of upregulated transcripts in roots and shoots categorised in the top twenty GOs are summarised. The
Analysis of
Because RPKM values obtained from RNA-Seq indicated significant upregulation of
Based on RNA-Seq, expression levels increased 341-fold in roots and 13-fold in shoots under –P conditions. In this study, we did not distinguish homeologous forms, and we present the results for each gene as an integration of counts from the three genomes of hexaploid wheat as reported by Pellny et al.
Figure 4
Expression of
Expression of
A highly conserved PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade
Members of the
A total of 7,449 transcripts were found to be orthologous to their corresponding rice genes based on a threshold of ≥ 60% identity and ≥ 60% coverage. In addition, BLASTN was used to identify rice orthologues of wheat non-protein coding genes. Orthologues of the responsive wheat transcripts were verified for their responses by comparing their data with those of the responsive rice transcripts. Because most well-characterised transcripts have been found to be upregulated under −P conditions in plants, we focused on the upregulated transcripts (Figure
The Pi regulatory mechanism has been elucidated through
Figure 5
Overview of the P-dependent signalling cascades that affect Pi remobilisation, Pi uptake, and changes in Pi metabolism
Overview of the P-dependent signalling cascades that affect Pi remobilisation, Pi uptake, and changes in Pi metabolism. The functions of wheat transcripts and rice orthologues of Pi-related transcripts were characterised under –P conditions. Asterisks show that the P1BS
We also studied the upregulated expressions of other responsive rice-orthologous wheat transcripts (Table
Genes involved in lipid metabolism, such as those that function in the synthesis of galactolipids and sulfolipids, were strongly induced under −P conditions. These include SQD1 (UDP-sulfoquinovose synthase 1)
Transcriptional control of other −P responsive transcripts
We identified several upregulated regulatory wheat transcripts involved in −P response from top hits to
Finally, we investigated downregulated wheat transcripts under –P and found that
In this study, we demonstrated the use of short-read sequence data to rapidly characterise a wheat transcriptome and have contributed significantly to the corpus of wheat transcript data. Differentially-expressed transcripts under –P in wheat included, on average, 23.4% of the contigs not covered by cDNAs in TriFLDB. The induction of these responses requires a sophisticated regulatory system, however, and details of this regulation in wheat have only recently begun to be elucidated. Comparison of upregulated wheat and rice transcripts revealed that the PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade is conserved in both crop species. Data from our previous study of rice transcripts and other supportive studies in other species confirmed that our analysis captured the transcriptome for –P response in wheat. This study thus represents a genomic approach to discovering wheat transcripts when genome sequences are unavailable. This contribution is significant to the development of genomic resources for wheat and other species and should accelerate the progress of functional genomic studies and breeding programs.
Plant materials and stress treatment
Seeds of the wheat cultivar 'Chinese Spring' were germinated and grown hydroponically in nutrient media (0.323 mM NaH2PO4; 10 mg L−1 P)
RNA isolation and quality control
Total RNA was extracted from all tissue samples using a RNeasy Plant Mini kit (Qiagen, Hilden, Germany). RNA quality was assessed using a Bioanalyzer (Agilent, Palo Alto, CA, USA); as suggested by the manufacturer, only those samples with RIN (RNA integrity number) scores greater than 8.0 were used in subsequent analyses.
Illumina sequencing and quality control
Twelve paired-end (PE) cDNA libraries were used to generate 107,298,935 PE reads from root and shoot tissues. Sequencing was performed on each library to generate 100-bp PE reads for transcriptome sequencing on an Illumina High-Seq 2000 platform. Library construction was accomplished using commercial products (Illumina), with sequencing performed by commercial service providers (Takara, Shiga, Japan). The sequence data generated in this study were deposited in the DDBJ Sequence Read Archive (Accession No. DRA000737). Low-quality bases (Q < 15) were trimmed from both ends using a customised program, and the adapters were trimmed using Cutadapt ver. 1.0 (
To generate a non-redundant set of transcripts, we performed a
Using a multiple
Calculation of RPKM values and the detection of differentially-expressed transcripts
The PE RNA-Seq reads from each treatment were aligned back to the cDNA, and the transcripts were assembled using Bowtie 2 ver. 2.0.0-beta6, with the parameters '-k 2 --no-discordant --no-mixed -q'. Expression levels of all unique transcripts mapped onto the full-length cDNAs and contigs were quantified using the RPKM values
Gene ontology analysis
The GO terms assigned to the responsive transcripts were obtained from InterProScan 5 Release Candidate 2 (
qRT-PCR analysis of wheat transcripts
First-strand cDNA was synthesised using a Transcriptor First Strand synthesis kit (Roche, Basel, Switzerland). Expression of Pi-upregulated transcripts was analysed by qRT-PCR in a LightCycler 480 system (Roche) with transcript-specific primers (Additional file
Additional file 6
PCR primers used for qRT-PCR analysis.
Click here for file
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
YO conceived and coordinated this study, performed the experiments, analysed and interpreted the data, and drafted the manuscript. FK, YK, and TY performed the general statistical analysis on the RNA-Seq data and participated in interpreting the results and in useful discussions. HH, TI, and TM provided valuable insights in the discussion and revision of the manuscript. All authors have read and approved the final manuscript.
We thank Ms. F. Aota, Ms. K. Ohtsu, and Ms. K. Yamada for their technical assistance.
Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource
Variation in phosphorus efficiency among 73 bread and durum wheat genotypes grown in a phosphorus-deficient calcareous soil
Identification of wheat alien chromosome addition lines for breeding wheat with high phosphorus efficiency
Mapping QTLs for phosphorus-deficiency tolerance at wheat seedling stage
Detection of QTLs for phosphorus use efficiency in relation to agronomic performance of wheat grown under phosphorus sufficient and limited conditions
Chromosomal control of the tolerance to soil phosphorus deficiency in genome of common wheat
Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots
Phosphate starvation triggers distinct alterations of genome expression in Arabidopsis roots and leaves
A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation
mRNA-Seq reveals a comprehensive transcriptome profile of rice under phosphate stress
TriFLDB: a database of clustered full-length coding sequences from Triticeae with applications to comparative grass genomics
Characterizing the composition and evolution of homoeologous genomes in hexaploid wheat through BAC-end sequencing on chromosome 3B
Megabase level sequencing reveals contrasted organization and evolution patterns of the wheat gene and transposable element spaces
Gene discovery and tissue-specific transcriptome analysis in chickpea with massively parallel pyrosequencing and web resource development
Optimizing de novo common wheat transcriptome assembly using short-read RNA-Seq data
De novo transcriptome assembly with ABySS
Oases: robust de novo RNA-seq assembly across the dynamic range of expression levels
Full-length transcriptome assembly from RNA-Seq data without a reference genome
Next-generation transcriptome assembly
Dissection of local and systemic transcriptional responses to phosphate starvation in Arabidopsis
Molecular regulators of phosphate homeostasis in plants
Target mimicry provides a new mechanism for regulation of microRNA activity
Cell walls of developing wheat starchy endosperm: comparison of composition and RNA-Seq transcriptome
A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae
OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants
PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants
Sumoylation, a post-translational regulatory process in plants
Discovery of barley miRNAs through deep sequencing of short reads
Phosphate utilization efficiency correlates with expression of low-affinity phosphate transporters and noncoding RNA, IPS1, in barley
Isolation and comparative analysis of the wheat TaPT2 promoter: identification in silico of new putative regulatory motifs conserved between monocots and dicots
Involvement of OsSPX1 in phosphate homeostasis in rice
RNase T2 genes from rice and the evolution of secretory ribonucleases in plants
Identification of rice purple acid phosphatases related to posphate starvation signalling
Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons
The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses
Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis
Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in Arabidopsis thaliana
Type-B monogalactosyldiacylglycerol synthases are involved in phosphate starvation-induced lipid remodeling, and are crucial for low-phosphate adaptation
Cytokinin represses phosphate-starvation response through increasing of intracellular phosphate level
Phosphate status affects the gene expression, protein content and enzymatic activity of UDP-glucose pyrophosphorylase in wild-type and pho mutants of Arabidopsis
Sucrose transport in the phloem: integrating root responses to phosphorus starvation
The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis
Identification and characterization of the Arabidopsis PHO1 gene involved in phosphate loading to the xylem
Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses
A putative novel transcription factor, AtSKIP, is involved in abscisic acid signalling and confers salt and osmotic tolerance in Arabidopsis
WRKY70 modulates the selection of signaling pathways in plant defense
Histone H2A.Z regulates the expression of several classes of phosphate starvation response genes but not as a transcriptional activator
SWI3 subunits of putative SWI/SNF chromatin-remodeling complexes play distinct roles during Arabidopsis development
Histone H2A.Z regulates transcription and is partially redundant with nucleosome remodeling complexes
The Swi/Snf family nucleosome-remodeling complexes and transcriptional control
Redundancy of chromatin remodeling pathways for the induction of the yeast PHO5 promoter in vivo
Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability
Phosphate Starvation Inducible; Bypasses' of adenylate and phosphate dependent glycolytic enzymes in Brassica nigra suspension Cells
The Calvin cycle revisited
Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development
Velvet: algorithms for de novo short read assembly using de Bruijn graphs
ABySS: a parallel assembler for short read sequence data
Optimizing de novo transcriptome assembly from short-read RNA-Seq data: a comparative study
Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences
Mapping and quantifying mammalian transcriptomes by RNA-Seq


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Daily Archives: March 26, 2013
Resident Evil Revelations trailer shows Rachel in action in Raid Mode
Capcom has released a new video of the updated Raid Mode from the upcoming console and PC versions of Resident Evil Revelations. The footage shows Rachel, who joins Hunk as one of the mode's new playable characters, in action. Other additions to the new versions include a harder difficulty mode titled Infernal, Residentevil.net integration, and new enemies, including the Wall Blister.
Dust 514 to get planetary conquest and more this May with Uprising
Dust 514 development continues to roll along, and developer CCP Games has announced a new milestone for the massively multiplayer first-person shooter, Uprising. The hefty update coincides with the tenth anniversary of Eve Online's launch, and brings the PC MMO and the free-to-play PS3 exclusive closer to complete integration. Not only will Uprising bring the universes closer together, but it will also improve the visuals, streamline the interface, incorporate planetary conquests, and add new battlegrounds, modes, gear, and weapons.


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U.S. Taco Co., Fancy New Taco Bell Concept: Blame Instagram
A pricey taco, some fries, and a milkshake are coming soon to a U.S. Taco Co. near you.
Coutresy of Taco Bell and Yum Foods
That's right, ya bunch of "foodies." Taco Bell's new attempt as cashing in on the upscale street taco trend is set to open this summer in Huntington Beach. However, the menu at U.S. Taco Co., which includes some interesting looking milkshakes, beer, and french fries for some reason, also has tacos priced as high as $7.
It's hard to imagine this concept surviving in a place like Phoenix with our already overabundant supply of cheap and authentic street tacos. And just how does Taco Bell justify those steep prices?
See Also: Taco Bell's New Waffle Taco: A Taste Test
According to TIME, Jeff Jenkins, a senior brand manager at Taco Bell, says that the times are a-changin' and Taco Bell is ready to dip their toes into the over-priced food market because your hip cell phone pictures prove that you're willing to shell out for it.
"We realized that at Taco Bell we weren't reaching all of those who want Mexican food," says Jenkins. "Everyone in the U.S. has become a foodie. The first thing young people do in a restaurant is take out their camera and take a picture of their food -- and post it on Instagram."
"This restaurant looks so Instagrammable that I am totally willing to shell out three times what I should pay," said no one ever,
Courtesy of Taco Bell and Yum Foods.
The TIME article says U.S. Taco Co. has ten planned taco options, one of which has lobster and another brisket, but we're holding out for the $25 gordita supreme with gold-encrusted chorizo and foie gras.
No word yet on when we can expect to see a Phoenix-area U.S. Taco Co., which makes you feel like your stuttering when said aloud, but we're guessing if our market couldn't support an amazing, inexpensive taqueria like Tacos Atoyac, then there won't be a U.S. Taco Co. for long.
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- Home
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- Future Research Needs: Prioritizing Research Needs in Gestational Diabetes Mellitus
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Future Research Needs for the Management of Gestational Diabetes
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Archived: This report is greater than 3 years old. Findings may be used for research purposes, but should not be considered current.
In January 2010, AHRQ requested that the Johns Hopkins University Evidence-based Practice Center (JHU EPC) develop and pilot test a process to identify research needs. The objectives of the project were: (1) to help AHRQ establish a standard process for identifying research needs in its evidence reports and (2) to identify and prioritize future research needs for the management of gestational diabetes.


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1978 GMC Sierra Classic 35
- Condition: Used
- Make: GMC
- Model: Sierra 3500
- Type: Extended Crew Cab Pickup
- Year: 1978
- Mileage: 139,000
- Color: Brown
- Engine size: 454
- Number of cylinders: 8
- Power options: Air Conditioning
- Fuel: Gasoline
- Transmission: Automatic
- Drive type: RWD
- Interior color: Tan
- Vehicle Title: Clear Want to buy? Contact seller!
1978 GMC Sierra 3500
This truck is a real barn find I bought this truck from an older woman that lives a couple miles from my house in Corning, a.. It was her husbands truck he passed away about five years ago. This truck has been parked in a barn sense then. Driven a couple of times a month to keep everything working.
He bought this truck at a car lot in Corning, a. in the mid 80's. This truck is in excellent original condition. The interior is almost perfect, ash has no cracks, ll gauges work, eats are like new with saddle blanket cover.
The engine was rebuilt at Lydon Johnson Motors in Corning, a. at about 120,000 so engine has about 20,000 on it. The truck has 139,000 on it. Runs excellent. The truck has a 454 engine with auto transmission.
It has an insulated camper shell on it. Wired with lights. The camper shell is in great condition. This truck does look like it came out of a time capsule.
I put a new thermostat in it just to be safe and a new alternator. It has a small exhaust leak on passenger side exhaust manifold. The rear fenders have some rock chips. There is some surface rust on the firewall behind engine. The filler neck on radiator were the cap screws on has a small crack. That is all I can find wrong on this truck.
Also have original tail gate that goes with truck.


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Indian Epapers >> English Epapers >> The New Indian Express
The New Indian Express Epaper
The New Indian Express is an Indian English-language broadsheet daily newspaper published by the Express Publications and based in Chennai. It was founded in 1932 as the Indian Express, under the ownership of Chennai-based P. Varadarajulu Naidu. Express Publications (Madurai) Limited publishes the The New Indian Express from 21 centres in Tamil Nadu, Karnataka, Andhra Pradesh, Kerala and Orissa.
Information about The New Indian Express NewspaperPublisher: Express Publications
Language: English
Newspaper Link: http://newindianexpress.com/
Epaper Link: http://epaper.newindianexpress.com/
Published from : Orissa,Chennai, Madurai, Tiruchirappalli, Coimbatore, Hyderabad, Bangalore, Kochi, Bhubaneswar and Shimoga
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Breakthroughs (formerly Crossroads) is a 13 week intentional path to freedom from the Walls in our lives. We refer to these Walls in our lives as Strongholds. Strongholds create bondage in our lives, rob us of God's peace and joy and cause us to feel "stuck" in life. Breakthroughs is designed to help individuals identify and tear down Strongholds thus leading to freedom in Christ. On this intentional path, we discover our identity in Christ and biblical truth that sets us free from lies of the enemy.
Breakthroughs includes a large group lesson each week, small groups and a workbook.
Breakthroughs meets on Wednesday evenings from 6:00 to 8:00 pm in room C234. New Session will begin September 6th, 2017.
Register for Breakthroughs (Fall 2017 Session)
Cost - $10 (includes workbook). Frequently Asked Questions about Breakthroughs
*Children's Ministries are available. See bottom of page.*
FREE TO BLESS YOUR SPOUSE – Discover how your own brokenness impacts your relationship with your spouse, how to receive healing and God's grace to move past the walls in your marriage and experience freedom to bless your spouse. This 7 week class is for any couple seeking deeper intimacy with God and their spouse while gaining understanding of how brokenness and spiritual warfare impacts their marriage. The purpose of the class is to help couples experience freedom to bless their spouse. Class taught by Marriage Counselor, Doug Valot and his wife Wendy.
Dates for next session TBA.
Duration: 9 weeks
Teacher: Pat Bramlett (Staff Counselor)
New Session dates TBD
Wednesdays from 7:00 to 8:00 pm.
Freedom Classes are topical classes focused on God's approach to freedom in Christ. Each class will explore an area that may be a stumbling block for living the life God designed for every believer. We will identify and learn how to remove the things that are currently hindering our spiritual growth. These classes are great for anyone interested in learning about freedom in Christ. No cost.
Topics for Freedom Classes
Marriage Matters is a new community group on Sunday mornings for couples of all ages. Marriage Matters is based on a 1-year marriage curriculum designed to help couples build a strong marriage using biblical truth and practical tools. Couples will discover the impact that experiencing true fulfillment in Jesus Christ has on their marriage and home. Couples will grow closer in their relationship with God and one another as they seek Him together. Led by Dan & Judi Post and Greg & Janet Burkett.
Join us on Sundays at 11:00 am in room C230 as we start a new study of of Kingdom Marriage by Tony Evans.
Griefshare is a 13-week group for those who have lost a loved one. Participants will watch a DVD lesson each week related to grief and loss and then have a time to share in their small group. The group will help individuals walk through the pain of grief and experience God's hope and healing.
Wednesdays from 6:30 to 8:00 pm in room C230 (2nd floor). Cost $12 (workbook included). *Children's Ministries are available. See bottom of page.*
Spring Session meets January through April 26th, 2017. New Session will begin September 6th, 2017.
Register for GriefShare (Fall 2017 Session)
Special Needs Parents and Caregivers Support Group - This group will encourage and support participants as well as provide helpful information through topical discussions each week. Group meets on Wednesday evenings from 6:00 to 7:00 pm in room C237 through May 10, 2017. *Children's Ministries are available. See bottom of page.*
Cost: None.
Register Here (Spring 2017 Session)
Journey - a 13-week Christ-centered, confidential support group for women whose husbands struggle with sexual purity. The group will use a workbook to help participants begin to heal as walk this journey with other women.
For information about similar local groups, contact Susan Sowell at firstname.lastname@example.com.
Surrendering The Secret - Abortion can be one of the most traumatic experiences in a women's life. Surrendering the Secret is a powerful off-campus 8-week study that helps hurting women find the path to healing through honest, interactive Bible study. With compassion, you will be encouraged and supported as you allow God into rooms of your heart that might have been sealed for too long. It's time to let go of the shame, accept His forgiveness and heal. All you need is to be willing. God wants to set you free.
ALL CONTACTS are CONFIDENTIAL.
Contact Mary Whittington for more information at email@example.com.
Surrenduring the Secret - Watch Video
Becoming One - Becoming One is a Community Group on Sunday mornings for couples who are engaged or newly married. Becoming One is designed around a 1-year marriage curriculum that helps couples build and grow marriages that reflect the image of Christ. Couples learn the importance of keeping their relationship with Christ the center of their marriage relationship. Class meets on Sunday mornings at 9:30 am in room C230 and is led by Ken and Pat Bramlett.
To help you and your family learn more about freedom, we offer Family Freedom Weekends two times a year. For information on our next Family Freedom Weekend, visit kingsland.org/familyfreedom.
If you didn't get a chance to join us for our Family Freedom Weekend in February 2017, you can now catch up on all the sessions! Click here watch all our main lessons. You can also download the audio of each breakout session!
For any questions about these classes, contact Jessica Molinari at 281-675-7862 or email@example.com.
*Children's Ministry Classes are available to your children. Visit www.kingsland.org/kingslandkids to read more.


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Brittany HigdonMakeup Artist Female Nashville, Tennessee, US
Mayhem # 457439
About Me
Hello, My name is Brittany and Im a little new to all this internet stuff so please bear with me. I am a professionally trained MUA and I am seeking work in the greater Nashville Area. I am available to do Makeup for Photography, Video and Events (such as Proms and Weddings).
I am sorry, but I am no longer available for TFP projects. Other than that, please give me a call if you need me!
Have you worked with Brittany Higdon? Add Credits for Brittany Higdon! >>
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About Servcorp UK - Virtual Offices & Serviced Offices | Servcorp UK
- Serviced Office +44 203 753 5500
- Virtual Office +44 203 753 5400
- Coworking +44 203 205 7333
- Meeting Rooms +44 203 753 5400
We'd love to help you find the best office solution for your businessCONTACT US
Company Information (Disclosure Regulations)
Servcorp UK Limited
Where registered: Greater London
Registered number: 034-56-047
Registered office address:
Level 17, Dashwood House
69 Old Broad Street
London, EC2M 1QS, United Kingdom
About Servcorp
At Servcorp we are committed to being the world's best Serviced Office and Virtual Office provider. Our business was founded on one principle - by reducing your costs and sharing your overheads, your business will succeed. So, as you focus on growing your business we give you the support you need to achieve your goals.
Servcorp not only gives you the ability to run your business from the best locations in the best cities around the world, but we also give you the best facilities, the best technology and the best people crucial to making your business successful.
Our team is proactive, efficient and on hand to support you. We believe in taking a genuine interest in the growth and success of your business.
We are proud to be an innovator of the Serviced and Virtual Office industry in our development of technology driven solutions which benefit your business.
Servcorp offers a range of Serviced Office and Virtual Office packages. Assisting both small and international businesses looking to expand into the London market without the hassle of sourcing, setting up and paying exorbitant rent for office space, Servcorp UK offers a range of services designed to suit any business needs.
Servcorp's world class facilities and infrastructure are an ideal solution for running any business in a prime CBD location. It also offers facilities for holding those important business meetings in a professional, corporate setting rather than the local coffee shop or a hotel room down the road.
Servcorp is the Serviced and Virtual Office industry leader in innovation and technology and can benefit your business, giving you more time and money to spend on your primary business activities.
At Servcorp we are committed to being the world's finest Serviced and Virtual Office provider by:
Our proactive and efficient team takes a genuine interest in your business and gives you the best customer service in our industry.
We believe in adding value to your business by exceeding your expectations and giving detailed attention to the little things. The Servcorp culture is built into every Servcorp location globally.
We're always available and at your door when you need us. We're just a phone call away when you're out of the office.


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Welcome To CK5!
Registering is free and easy! Hope to see you on the forums soon.
Score a FREE Membership sticker when you sign up for a Premium Membership and choose the recurring plan.
Need camo paint scheme
Discussion in 'The Garage' started by txbartman, Jul 20, 2002.


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You can go away on your trip knowing that I will be taking care of your space while you are away. Your mail will be retrieved, your plants watered, and your pets cared for and fed. (I'm mainly a dog/cat person, so I might not be your best choice outside of those pets.
I will be sure to leave your house as you left it upon you return. Leave no trace.
Training & Qualifications
I'm a mature, caring individual, who work's during the day (either from home or at the office.) I will be sure to determine the best way of caring for your home (and pets) with your help ahead of time.
Reviews of Doug
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About Doug Miller
San Francisco loving Midwestern boy. Love music, bicycling, all things tech, and doing the right thing. Handy at a fair number of things, and looking forward to sharing my skills with the Simbi commun...ity.show moreOpen to other proposals
Doug Miller is looking for:
- Music & Audio
- Experiences
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Dancing in the Footlights
Moments of Music with Jonathan Urie
Recent Broadcasts
July 26
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Featured Offer from Jonathan Urie
Just Enough Light for the Step I'm On Thousands of readers have been touched with the encouraging message found in Stormie Omartian's bestselling book Just Enough Light for the Step I'm On (over 400,000 sold). Now in a deluxe padded hardcover version with a bookmark ribbon, this encouraging look at God's faithfulness will help more readers overcome fear and doubt in difficult situations learn to see things from God's perspective believe that God gives His children everything they need embrace the purpose and plan God has for them stand on the promises found in the Bible.
While sharing openly from her personal exper-ience and her strong understanding of God's Word, Stormie illuminates the way for readers to turn their concerns, trials, worries, and daily needs over to God's care.


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The Phantom Witch is a pyrokinetic ghost that terrorized a part of Queens.
The Phantom Witch was a fat, wailing spirit of a witch that was able to fly and produce an explosive, dangerous orb of green energy out of her mouth. She haunted the elevated tracks at the Sunnyside Yard in Queens. At around 6:30 am, [1] the Extreme Ghostbusters pursued the phantom. Eduardo Rivera almost got killed capturing her but Roland Jackson confined her in a Proton Stream and Kylie Griffin secured her into a Trap.
Besides her ability to fire explosive orbs of energy from her mouth (which are referred to by Eduardo as "ecto-grenades"), [2] the Phantom Witch can also fly and phase through solid matter like most ghosts.
- Egon Spengler refers to the bust as an "incendiary spectral situation." [3]
- ↑ Kylie Griffin (2009). Extreme Ghostbusters- The Unseen (1997) (DVD ts. 5:50-5:54). Sony Pictures Home Entertainment. Kylie says: "...Under the elevated tracks in Sunnyside around 6:30 this morning."
- ↑ Eduardo Rivera (2009). Extreme Ghostbusters- The Unseen (1997) (DVD ts. 6:54-6:56). Sony Pictures Home Entertainment. Eduardo says: "I almost took an ecto-grenade for you, lady."
- ↑ Egon Spengler (2009). Extreme Ghostbusters- The Unseen (1997) (DVD ts. 4:45-4:47). Sony Pictures Home Entertainment. Egon says: "Ghostbusters, did you resolve the incendiary spectral situation?."


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£295 pcm Available Now
Ground floor flat situated in Largs consisting of Lounge/Kitchen, double bedroom and bathroom, shared garden to rear, situated close to shops, library, buses and train. Landlord Registration Number 35495/310/20450.
This house description is based upon information supplied by the owner, or on behalf of the owner. These property particulars are produced in good faith and do not constitute or form part of any contract. s1homes do not take any responsibility to the accuracy of the information contained in this document.


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Job Description – Project Manager
No. Of Openings: 2
JOB DESCRIPTION (KRAs)
- Plan, execute, manage, and complete medium to large IT projects that address the
operational needs of two or more departments.
- Develop and oversee the resources to ensure the appropriate management, customer and
supplier involvement for life of project.
- Be accountable for project results along with the team members.
- Work with team and client to complete project charter outlining scope, goals,deliverables, required resources, budget, and timing.
- Ensure documentation is maintained as the project is changed or refined over time and
communicate the project status to end-users.
- Act as a mediator between clients and team members to clearly communicate expectations.
- Effectively manage project scope by ensuring any changes to scope are documented.
- Track and report on-project milestones and provide status report.
- Ensures all internal and external standards and regulatory requirements are met.
- Work very closely with our business analysts.
- Provides feedback to team and/or individuals on performance.
- Bachelor's degree in Computer Science, Management Information Systems, or a related field.
- The candidate must have a minimum of 4 years of experience in project management.


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Etherington Review of Fundraising
Over the summer, eight consultation questions were published. The deadline for responses was 14th August and the panel is due to report to the Minister of Civil Society by 21 September 2015. The consultation questions were:
- What do you consider to be the strengths and weaknesses of the current self-regulatory set up? Do you believe self-regulation continues to be an appropriate approach to regulating fundraising?
- What do you consider to be the strengths and weaknesses of the bodies currently involved in self-regulation?
- What changes, if any, do you believe should be made to the current self-regulatory structure?
- What do you consider the most effective ways to ensure coverage of and compliance with a self-regulatory regime?
- How could it best be ensured that a future self-regulatory system is adequately resourced?
- Which charities should be covered by self-regulation? Should there be a threshold for fundraised income before membership of a self-regulatory body is expected? If so, where would you set this threshold?
- Should additional measures be put in place to monitor or regulate operational fundraising agencies, such as call centres? If so, what should these be?
- Do you have views on how to ensure charities adhere to high standards in public fundraising, other than through formal regulatory structures?
Please click here for more information.
Parliamentary inquiry into charity fundraising
This was announced on 21st July. The inquiry will focus predominantly on four key areas:
- the extent and nature of practices adopted by call centres raising funds for charities and the impact on members of the public, particularly vulnerable people
- the Government's recently proposed legislative changes on this issue
- how charities came to adopt these methods, and how they maintained proper governance over what was being done on their behalf
- the leadership of charities and how their values are reflected in their actions and activities
The committee is seeking written evidence and submissions on the issues from charities - the deadline for submission is noon on Wednesday 26 August 2015. An evidence session will be held in early September.
Review of Scottish Fundraising self-regulation launched
The Scottish Council for Voluntary Organisations has launched an informal review of fundraising in Scotland to assess whether or not the current system of public charity fundraising self-regulation is working.
The review, which will report back by the end of August, will be led by a reference group made up of third sector representatives and will also include a survey on views about fundraising among members of the public, charities with an interest in the sector's reputation, charities who fundraise and professional fundraisers.
Changes to Institute of Fundraising Code of Practice
The following key changes have been announced:
- an amendment in relation to calling TPS registered numbers. This change was announced on 18th August and takes immediate effect. It follows a summit on telephone fundraising practices which took place on 31st July. The FRSB has welcomed the change.
- on 1st September, the new rule relating to 'No Cold Calling' stickers comes into effect.
Separately, the Direct Marketing Association has published:
- updated guidance on dealing with vulnerable consumers.
- a set of free training materials for telephone contact centre agents on how to deal with vulnerable people, including flash cards summarising the characteristics of some of the most common vulnerabilities.
FRSB/PFRA news
Professor Andrew Hind CB has been appointed as the new Chair of the Fundraising Standards Board, with effect from the FRSB Board meeting on 16th September 2015.
From 1st September, the Public Fundraising Regulatory Association will be known as the Public Fundraising Association. While the organisation will continue to be known by its PFRA acronym, and carry out all its existing functions as before, the move is intended to provide extra clarity to the sector and wider public.
Wills and Legacies
This is a link to the full Court of Appeal judgment in the much publicised case where the Court reassessed an amount to be paid to an estranged daughter from her mother's will. The daughter had brought a claim under the Inheritance (Provision for Family and Dependants) Act 1975.
Christine Rigby
Senior Consultant
- +44(0)20 7551 7712
- email@example.com
Posted on 01/08/2015 in Legal UpdatesBack to Knowledge


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Two arrested on drug charges after Grass Valley traffic stop
May 9, 2013
Grass Valley Police officers arrested a man and a woman on drug charges after a traffic stop Sunday afternoon. The woman later was linked to a case involving forging drug prescriptions, said Grass Valley Police Capt. Rex Marks.
An officer conducting extra patrols due to reports of increased drug activity saw a suspicious vehicle on Buena Vista Avenue at about 4 p.m., Marks said.
During a search of the vehicle, officers allegedly found a little more than 1 gram of methamphetamine, as well as miscellaneous pills believed to be prescription medication and drug paraphernalia.
The driver, Kyle Gower Short, 18, was arrested on suspicion of possessing a controlled substance and drug paraphernalia and was booked and released on $11,000 bail. He also had been arrested in March by Grass Valley Police on suspicion of possession of stolen property and counterfeit bills.
The passenger, Jessica Marie Schenck, 27, was arrested on the drug charges, as well as for charges in connection with a prior case involving forging prescriptions, Marks said. She was booked and released on $11,000 bail.
Schenck also had been arrested in early April after Grass Valley Police officers allegedly found her with 30 grams of methamphetamine and drug scales.
Schenck was stopped in the 500 block of Brunswick Road at about 1:30 a.m. and allegedly appeared to be under the influence of narcotics. During a search of her person, officers found 30 grams of methamphetamine and scales.
To contact Staff Writer Liz Kellar, email firstname.lastname@example.com or call 530-477-4229.
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RepairPal is your advocate for car care advice and guidance
Q: just hit 40,000 now brake pedal slowly goes to floor. on 2006 Ford E-250
Replaced master cylinder with a brand new one and bled everything out real good. pedal is better but still creeps slowly to the floor. no leaks anywhere, is it a bad new master cylinder or is there somewhere else that system can leak internally and not be noticed?
What does the pedal do if you pump it without starting the engine? Was the old M/C leaking between it and the booster? If so , poss. problem with booster. Also was there any adjustment on pushrod between M/C and booster?
Engine not running pump and hold the pedal and it still goes to the floor. not leaking between the master cylinder and booster . so the pedal still studly goes to the floor whether the motor is running or not.
How to Fix Your Brakes
Video, Step-by-Step Instructions, Parts & Tools List
Video, Step-by-Step Instructions, Parts & Tools List
possibly a bad new one...
thats what I'm thinking . I have rebled the system and double check everything . whether the motor is running or not the pedal still slowly goes to the floor. this is why I bought a brand new one instead of rebuilt one to begin with .
Disconnect the M/C and plug the ports (probably with the plastic caps that it might have come with) and bleed just the M/C like that and see what it does--If you get a good pedal , then you've got a leak somewhere else. If the pedal still sinks without any fluid leaking by the plugs than you've got a bad M/C. Was the part from Advance Auto Parts? If so get your money back and get one from NAPA.
Bought it from napa , bled system one more time and got more air out. Drive it tomorrow and if peddle still
Goes to floor I will plug it off and try it soley like you suggest, that is a great idea.
Goes to floor I will plug it off and try it soley like you suggest, that is a great idea.
I hope you get it figured out. I also hope for my sake (not yours) that it is not the M/C since you already got it where I suggested.......always had the best luck with their parts. For your sake , whatever it is , I hope it's not a pain in the.................
Latest update on my brake problem,I have replaced my new master brake cylinder from Napa with another new one from Napa and bled on bench and bled all four wheels real good. Got a lot of air out but samr problem, stops fairly good but right away peddle starts fading to the floor and I have to keep pumping to hold at a stop sign.
all the way around are disc. new brakes on front and rear are about half.
Didn't find what you were looking for?


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If 2K Games told you to jump off a bridge, would you do it? Well, that's exactly what they're asking you to do on Sunday September 23rd, with the announcement of a truly insane event to celebrate the launch of Borderlands 2.
All are invited to test their mettle by dropping from a specially designed Borderlands 2 bungee crane, which will be located at Ely's Yard, Brick Lane, London. Ben Lawrence, UK PR Manager for 2K Games, explains:
"We want to provide an afternoon of extreme fun and discover just how psycho Borderlands 2 fans can get. A bungee jump is the perfect way to truly test your nerves and we're excited to bring the insane pleasure of the game into the real world!"
The jumps will be on a first-come first-served basis, taking place between 10:00am and 4:00pm, starting at the Old Truman Brewery, London. Fearless enough to blag a freebie? Get on down and be sure to tweet us with pictures if you go for it!
Borderlands 2 is scheduled for release on September 21st for Xbox 360, PS3 and PC.


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The stability of viscous flow under an axial pressure gradient between two co-axial cylinders rotating in the same direction is considered. The critical Taylor number (T$_c$) for the onset of instability is then a function of the Reynolds number (R) of the mean axial flow. A perturbation theory valid in the limit R $\rightarrow$ 0 is developed and the formula $T_c(R) = T_c(0) + 26\cdot 5R^2 (R \rightarrow 0)$ is established.
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