text:
Google is now an ICANN-accredited registrar of domain names,
providing it with yet another potential line of expansion and further
fueling speculation that it will soon expand into the web hosting
The hugely popular and resourceful search engine is approved to sell
names in seven top-level domains (TLDs) including .com, .net, .org,
.biz, info, .name and .pro.
Domain registration, combined with the already operating a free blogger
service, and its huge user base would be a potentially lucrative market
for domain names and Web hosting. Additionally its other beta services
such as gmail and Google News allow for even further expanasion
Over the last year, significant reductions in domain name prices have
been spawned by the use of domains as marketing tools to attract new
web hosting customers. Industry leaders – Hostway, Yahoo!, and
Interland , as well as most medium and small web hosting companies,– are selling domains as low as $3.95, but none are accredited
registrars. Instead, they work as resellers for firms such as Go Daddy.
With prices as low as they are, domains names have, in most cases,
become a losing financial situation, but are seen as a cheap and
effective way to attract hosting customers. The low loss on a domain of
a couple dollars, versus the pay-per-click cost of buying the keyword "web hosting" on Google Adwords or Overture, which can run three to
four times higher.
While competing with their customers may never have crossed Google's
mind, the simple economics of Web hosting will necessitate a different
approach. Google could easily give up the number 1 advertising slot to
market a Google Web hosting package. Additionally, Google could simply create a new ad unit that rests at
the very top of the page, preserving all existing advertising slots.
Adding thousands of new hosting accounts each month will quickly
generate millions in recurring revenues for Google. Yahoo! did this a
few years ago with astounding success.
A great hosting company needs to stave off DDOS attacks, worms,
viruses, and a litany of other hacker-induced mayhem. It also needs
super-fat redundant pipes to handle all the traffic, and the additional
mandatory goodies like physical security, fire suppression, and backup
generators to maintain 100% uptime. Google has been protecting a
premier Web property from hackers for the last 6 years. With their
experience in data centers and knowledge of redundant connectivity, the
financials already exist.
Gmail! Okay, so they messed up a little with the whole privacy/big
brother issue. They'll get past that. The point is that Google knows
email. Even with the throttled launch and invitation-only system of
Gmail, they probably already have more email customers that most
hosting companies. This gives Google 2 more clear reasons to get into
hosting: they can handle email as well as anybody, and they have a huge
email list to which they can market their service. Many customers
register a domain name and set up a hosting account mainly to have the
email address that they want, such as firstname.lastname@example.com. The ability
to process and manage a high volume of email while excluding spam,
viruses and other security exploits is a mandatory skill set for
Why Will Google's Web Hosting Product Reign Supreme?
*According to a article on Sitepoint
It's pretty much a given that Google will launch a Web hosting product
at some point. But how will they crush Yahoo! (and every other Web host
out there)? Here are a few points on which Google trumps its nearest
– Google brand versus Yahoo! brand. Winner = Google. Google is regarded as cutting-edge and is closely watched. Yahoo! has repositioned itself within the market and is not the tech leader is used to be.
– Google's execution versus Yahoo's execution. Winner = Google.
Google's awesome tactic of launching full blown, fully developed
products as 'beta' tests overcomes the pressure and scrutiny of
conventional product launches. Plus, they're just really good at
executing Web-based technology.
– Google's reach versus Yahoo's reach. Winner = Google. By some
accounts Google now accepts 70% of all Web searches each day. Unless it
stumbles, Google will keep growing at Yahoo's! (and everybody else's)
– Google's synergies versus Yahoo's! synergies. Winner = Google. Google
bought Blogger, and it already knows and understands how to attract,
market to, and partner with content builders. Google's AdWords and
AdSense products are already in use by well over 150,000 individual
Websites. Google has made a science out of knowing which markets are
searching for what, and where they go to find it.


text:
U.S. forces "can't stay in front on this over the long haul and be successful," Army Gen. George Casey said. As long as many Iraqis view the Americans as "an occupation force," it will help feed the insurgency, Casey said.
As Iraq heads toward elections on Sunday, Casey told reporters that he expects U.S. training of a new Iraqi army and police to accelerate this year. Since June, the Iraqi military has gone from fewer than 1,000 troops who were trained, organized and equipped to more than 60,000.
The total number of Iraqi security forces, including police, is about 130,000.
But even though more Iraqi troops are fighting — instead of running away in the face of danger, as many did last year — they are not prepared to take over command from the U.S.-led coalition.
"Are they capable of taking over the counterinsurgency campaign today? The answer is no," said Casey, commander of the multinational force in Iraq.
What's needed, he said, is not only trained foot soldiers but commanders who can manage a military campaign. To develop those skills, he said, larger numbers of U.S. officers will be placed as advisers with Iraqi forces.
One of the issues in the Iraqi election campaign — and facing the next government — is when U.S. forces will leave Iraq. Under the United Nations resolution covering the creation of a new Iraqi government, Iraq will have the right to demand that all foreign troops leave the country.
Interim Prime Minister Ayad Allawi said this week that he would not ask the Americans to leave until Iraqi forces were prepared. A new government could change that position.
Sunni Muslims, who form the core of the insurgency, have been demanding at least a timetable for a pullout.
"We want to leave the Iraqi security forces in a position where they can sustain the success that we've had," Allawi said. The test will come when Iraqi troops are leading the counterinsurgency effort, instead of just accompanying U.S. forces as they do now, he said.
Casey said it would be wrong to set a deadline for reducing the U.S. forces.
In the aftermath of the U.S. offensive to drive rebels from Fallujah, he said, at least 60 major figures in the insurgency, including aides to terrorist mastermind Abu Musab al-Zarqawi, have been arrested.
Casey said 15,000 insurgents have been killed or detained in the past seven months, and that the number of insurgent attacks is down from its peak in the fall.
He estimated that fewer than 1,000 of the insurgents, including the Islamic extremists loyal to Osama bin Laden, were foreigners.

subset: commoncrawl


text: TV Syd is one of TV 2's eight regional services. It is headquartered in Kolding although it was previously based in Haderslev. TV Syd was launched in 1983 as an experimental regional channel operated by Danmarks Radio (DR). Upon the launch of TV 2 in 1988 it was immediately co-opted into the TV 2 network as its first regional service.


text:
"Baby Doll" Ragini MMS 2 Sunny Leone Song | Meet Bros Anjjan Feat. Kanika Kapoor MP3
Presenting the very first video song "Baby Doll" from the bollywood most awaited movie "Ragini MMS" starring Sunny Leone in lead role. Click to share it on ...
La Femme - Oh Baby Doll MP3
Full song for the Yves Saint Laurent Babydoll Mascara Ad (see below): Original Ad: http://www.youtube.com/watch?v=_kgWokCQSBs A Chuck Berry song ...
Chuck Berry - Oh Baby Doll (Alan Freed's Mr. Rock and Roll) MP3
Chuck Berry (Charles Edward Anderson Berry - born Oct. 18, 1926, St. Louis, Mo., U.S.) singer, songwriter, and guitarist who was one of the most popular and ...
Hurriganes - Oh Baby Doll MP3
Remu Aaltonen: Vocal, Drums. Ile Kallio: Guitars. Cisse Häkkinen: Bass.
Hurriganes - Oh Baby Doll MP3
Single 1976 Love Records. Remu Aaltonen, drums, voc. Cisse Häkkinen, bass. Ile Kallio,gtr.
Chuck Berry - Oh Baby Doll. MP3
Chuck Berry - Oh Baby Doll.
Chuck Berry - Oh Baby Doll MP3
Chuck Berry Oh Baby Doll - 1957.
Baby Doll - Danny Collins (Al Pacino) Full song with Lyrics MP3
Baby Doll, by Danny Collins - Al Pacino- Danny Collins movie. LYRICS: Hey! Baby doll What's going on? Sweet baby doll I sing my song for you You were ...
Ile Kallio - Oh baby doll MP3
Remun synttäribileistä.
Chuck Berry - Oh baby doll MP3
Chuck Berry - Oh baby doll Lyrics/Letra: Oh baby doll! When bells ring out the summer free Oh baby doll! Will it end for you and me? We'll sing our old Alma ...
hurriganes - oh baby doll MP3
Bob & Earl - OH BABY DOLL (Blossoms) (1962) MP3
NO COPYRIGHT INFRINGEMENT INTENDED - (Tempe 104) A year before Bob & Earl would have a hit with 'Harlem Shuffle' this 1962 disc was recorded in Los ...
Hurriganes - Oh Baby Doll - Live 1981 MP3
Remu Aaltonen - Vocals, Drums Albert Järvinen - Guitar Cisse Häkkinen - Bass.
Oh baby doll - Pretty Things MP3
Track from their first LP 'The Pretty Things', released in 1965.
TOP CATS "Baby Doll" (New single February 2012) MP3
Spotify: http://open.spotify.com/album/4f5iotMXSoTvH7GhX8yJmf.
Baby Doll - LYRICS | Ragini MMS 2 MP3
Al Pacino - Hey baby doll (2015) MP3
Chuck Berry Oh Baby Doll MP3
Chuck Berry Oh Baby Doll.
THE CRAWDADDYS - Oh Baby Doll MP3
From San Diego (California) -1979.
Oh Baby Doll MP3
Rude Runners (Hurriganes Tribute) Live at Santa Fe, Tammisaari 16.8.2014 Remu (Saska Ketonen) Ile (Lasse Parkkila) Cisse (Mikki Niinivaara)
Truck n roll Oh baby doll Hurriganes cover MP3
hurriganes hurriganes get on hurriganes i will stay hurriganes roadrunner hurriganes bourbon street hurriganes do you wanna dance hurriganes tallahassee ...
Chuck BERRY "Oh Baby Doll" (1957) !!! MP3
RARE OLDIES VIDEO WITH MR CHUCK BERRY ! Charles Edward Anderson "Chuck" Berry (born October 18, 1926) is an American guitarist, singer and ...
The Pretty Things - Oh Baby Doll - 1965 MP3
Chuck Berry's song Pretty style.
Baby Doll Mein Sone Di - Ragini MMS 2 - Sunny Leone HD MP3
http://www.Date2Rishta.com - The Fastest Growing Site...For Single South Asians Baby Doll Ragini MMS 2 Sunny Leone song Mein Sone Di - Ragini MMS 2 ...
Aqua - Barbie Girl MP3
Music video by Aqua performing Barbie Girl. (C) 1997 Universal Music (Denmark) A/S.
Barbie Girl
AquaHiya, Barbie! Hi, Ken! You wanna go for a ride? Sure, Ken! Jump in! I'm a Barbie girl in a Barbie world Life in plastic, it's fantastic You can brush my hair, undress me everywhere Imagination, life is your creation Come on, Barbie, let's go party! I'm a Barbie girl in a Barbie world Life in [...]
FB/Blue Rag Soldiers Ent.
Oh Baby Doll - An Awful Crime / Black Devils MP3
Alive at Totem, Lappeenranta, Friday the 22nd of April 2011.
Big Uh-Oh with RealCare 2 Baby - Doll Break Ep. 75 MP3
While fixing the eyelashes on my Real Care 2 Baby Think It Over doll, Hayley, I discovered something shocking! A BIG Uh-Oh on my part! :( Get your own "Keep ...
THE PRETTY THINGS ¨Oh Baby Doll ¨ MP3


text: Y'all is so far off it ain't funny. How do I know? I done seen her down Barberton way. Infact, I was half my plum head into a game of "just the tip" with her when some dude she kept calling spanky called her up and she started talking about nose jobs. She told me to relieve in her nose hole and that it wouldn't be disrespectful cause she was on her last couple nights with that nose anyways.


text:
Burt Bacharach's ex-wife changed for him
Burt Bacharach's ex-wife wore lifts in her shoes, had a boob job and changed her style in order to please the singer.
Carole Bayer Sager admitted she felt insecure about her 5ft 1in height, weight and appearance and offhand remarks from the singer didn't help her feelings.
In her new book, 'They're Playing Our Song', the 69-year-old singer recalled how she and Burt were heading out for dinner one night when he remarked on how her skirt made her legs look short.
She said: "I stood there semi-stunned, my fragile-at-best self-image melting as he went on. That was it. It only took one remark, from someone I wanted to love me, for me to cover a body part for the rest of my life."
After they had been together for four months, Burt gave Carole her first gift - a pair of framed photographs, one of a racehorse and another of a woman with her legs crossed provocatively.
She recounted: "All I saw was his worship for beauties that, at five foot one, I could never be. He didn't mean to be hurtful. He was just oblivious to the effect of his actions."
On Dolly Parton's recommendation, Carole - who wrote a number of songs with 88-year-old Burt - contacted shoemaker Pasquale Di Fabrizio, who gave her spouse two extra inches and a third secretly-built into his shoes, while his footwear elevated Carole by a further 41/2 inches.
After Burt moved in with Carole, he insisted on keeping his own one-bedroom apartment, and when she visited that property one day, she found a number of slides of a "naked, full-breasted woman" with her phone number written on them, but her spouse dismissed the pictures as being from a "crazy fan" and insisted he had never called her.
Carole then asked if he had a thing for big breasts, prompting him to say: "What guy wouldn't?"
According to the book, he then told her he'd had a conversation with second wife Angie Dickinson about her, in which he said: "I must really be in love with her because she doesn't even have big breasts."
After the conversation, she then sought the services of Dr. Harry Glassman for "perky and alluring" new breasts, before getting the bags under her eyes removed.
In an extract from the book obtained by DailyMail.com, she noted: "It was always only a little 'fix', but that's what I was discovering it was all about. Little fixes - maybe a shot of collagen here, a bit of Botox there.
"I was just trying to look like I belonged with Burt'.
Carole and Burt - who have adopted son Christopher together - divorced in 1991 after nine years of marriage.
Carole has been married to American Film Institute chairman Robert Daly since 1996, while Burt wed fourth wife Jane Hansen - the mother of his kids Oliver and Raleigh - in 1993.


text:
We booked rooms in February for a July trip. We had to make a deposit of the full cost at the time. The receipt we were sent says that cancellations must be made at least 24 hours in advance. There are no other conditions. We...More
Getting you more information on this room
Getting you more information on this room
"I was on the second floor, back side with a few of woods...perfect for me."


text:
Karate Kubs Program (Ages 4 to 6)
The Karate Kubs program is designed for children four to six years of age. This program is a preparatory course for the junior Taekwondo classes. In addition, it also focuses on life skills such as social development, gaining independence, and following instructions that will benefit the student in their pre-school and kindergarten classroom.
This is a fast-paced class with a specialized curriculum for children of this age group. The students will enjoy an exciting class while learning essential lessons in self awareness and protection. The goal of the Karate Kubs is to provide a structured environment with activities that enhance physical maturation and coordination, attention span, social skills, safety awareness and self-discipline to children in this age group.


text:
This new Nokia mobile phone is fitted with the new Nokia Maps 2.0 for GPS including a built-in compass and additional 'accelerometer' that measures changes in direction and orientation and full voice guidance. The included tariff offer not only provides a great selection of free minutes but also reduces the basic line rental down from £35 to £30 and also cuts the first 3 months down to £15. In addition, the unlimited landline feature could save you hundreds over the life of the contract.
Click here for Nokia 6210 Navigator from Vodafone deal
[Wait until window loads then scroll down to deal]
Click here for more news


text:
It's like I was from Mars, and EA was from Venus.
College Hoops understands me. If I lose in 2K's game, it's because I didn't move the ball efficiently enough, I didn't take my shot when I had it or I missed my free throws when it counted. There's a strategy in 2K's monster beyond holding turbo.
That's awesome.
Of course, like any solid relationship, there are growing pains. When 2K and I first hooked up with College Hoops 2K6, I thought the title had a good personality - tight controls with bright AI - but was let down on the overall promise. See, 2K6 promised an authentic college basketball experience but featured players that seemed as if they just stepped out of the Speed Force. Guards and centers would shoot up and down the court in a way that made every play feel like a fast break and detracted from the satisfaction of working the ball around the perimeter to the open shooter.
2K7 fixed my players' need for speed. You can hold R1 for a boost, but any other time your players are moving at a reasonable speed to set up screens and work out plays. Although the rest of the controls are pretty run of the mill, the use of the Six Axis for free throws - tilt the controller back and rotate it forward as the player shoots - takes some getting used to but ultimately is more satisfying and challenging that 2K6's similar use of the analog stick.
Getting your legacy team on a roll takes a detail-oriented eye. As you navigate your calendar and delegate which coach is recruiting what player, you also have the option to monitor your current roster. If one player is down, you might want to consider doling out one of your ten pep talks. When game day comes around, a Unity meter in the lower left corner of the screen tells you how well your team is meshing. Substitutions will either add or detract from their cohesion and that will equate to success on the scoreboard.
When your heart starts racing and your team goes on a run, be prepared for your opponents to lose composure and start bumbling - when my Tigers would get a kick start, terrible passes, bad shots and out-of-position players became the hallmark of my rivals.
The action on the court is mirrored by the clamor of the crowd. Although I could have done with a more active throng of hardcore hoop fans rather than the placid crowd in 2K7, their vocal intensity is unbeatable. Play in the house Coach K built, and the Cameron Crazies get louder and louder as the Blue Devils pull away. Head to Kansas to face the Jayhawks, and the crowd inside Phog Allen Fieldhouse breaks out their eerie chant of "Rock Chalk Jayhawk."
If you&#Array;re a college basketball fan and you own a PS3, there&#Array;s really no reason you shouldn&#Array;t buy this game. The Legacy mode is deep, you can create your own online leagues and when all that gets boring, you can try to make dirty chants in Chant Creator. It&#Array;s the basketball title that keeps on giving.


text:
Club Support Grants
NYSSRA-Nordic aims to financially support clubs that host events, grows NYSSRA membership, and maximize participation in the many events offered throughout the year. If you are a club organizer, please read the Club Support criteria and plan on applying for a club grant at the end of the race season. To fill out an application please visit one of the three links:


text:
What is the normal life expectancy after triple bypass surgery?
Studies have shown in 80 to 95 percent of patients, bypasses made with segments of the mammary artery still performed efficiently 10 years after surgery. Two-thirds to 75 percent of patients who received grafts from leg veins still had satisfactorily unrestricted blood flow after 10 years.
Remember that even if your graft becomes blocked, you may not need additional bypass surgery. However, you may be a candidate for balloon angioplasty and insertion of a stent.
Ultimaately, there is no textbook answer to this question as it depends on many factors. These include your condition going into the surgery, the particular surgery, your particular recovery course, any complications that might have occurred post-operatively (delay in healing, infection, abnormal cardiac rhythms), other underlying medical conditions, and your doctors. The best outlook would come from your surgeon and/or cardiologist, as they are familiar with your condition and case.
This question can't be really answered correctly. There really isn't any answer to it. Everyone and everyone's situation is completely different. Plus, there is a lot to consider. Have you EVER heard of someone dying because of a Triple Bypass? People of all ages can have this surgery, but the biggest percent are really old. People that have this surgery may have NO other heath issues, but the biggest percent of them have many other health issues. People that have this surgery also may or may not live a healthy life, many may over eat, eat unhealthy food, not exercise, and not take perscribed medications. Theres a lot of talk about "how long a bypass will last", but you never really hear of someone dying because their bypass failed after 20 years. And there are some people that had a bypass, and years later had more bypasses and lived many years later. They may say that a big majority of Triple Bypass people are only expected to live 10 years after, but thats because the biggest percent of them are over 70yrs old and they die of old age or some other health problem with-in 10 years after. There is many healthy people that have had a triple bypass that have lived many many years after, over 40 years after before dying of something else. Heart Surgery may sound very scary, but it's a great life saver. Most people die of a heart attack or heart failure, they don't live to get the hospital to get treatment, they didn't even know they had any heart trouble. Having a Triple Bypass means you made it, you have a second chance at life and your not going to be one of the millions that just have a heart attack and die with-out getting treated. READ MOREAnother thing to think about, is that even if 20 yrs later one of the bypasses fail, the human body is an amazing thing, and the heart as it gets older makes more and more blood vessels like spiderwebs. So, when an area of the heart may have had only one artery supplying it the oxegen that it needed, years later that same area may have several vessels supplying it oxegen. A really old person could even have a totally blocked artery, which means he had a heart attack, but he might not feel it and it may not even effect him, as the same area of the heart is getting oxegen from several other blood vessels. READ MORE
I don't want to rain on everyone's positive vibes, but I had a triple bypass at 55, and now 11 months later, two of the three grafts have failed, and I'm left with few options. They say it's rare this happens, and mostly with females, which I am, so I guess I'm just the one that had the bad luck this time.READ MORETRIPLE BY PASS AND VALVE REPAIR ON A 87 YEAR OLD MAN. GOOD IDEA OR BAD? READ MOREI had a bypass 34 years ago it lasted 24 years since then I have had 5 sinits I am just curious to know if there are any who has lasted longer. READ MORE
I personally don't know, but I had one 6 weeks ago, not a planned one. went in for a stress test and wo la... anyway, my voice has NOT returned to normal and it has been 6 weeks. anyone else have this or what to do? people can't hear me when on phone or some evn in a room
Hi, I had a tripple by pass in may 2001 (12 years ago) and apex of my heart muscle is dead.... can I still have another open heart surgery if necessary.... I am now on Imdur 30 mg for about 3.5 years and its helping.... my last MIBI nuclear test shows that my heart is working good.... also I take aspirin, beta block, vasotec and lozec
I am really worry what would happen if I had another heart attack....
see my by-pass was complicated by having 3 seizures as I had 16 years before my CAB repaired brain aneurysm....
can you give me some advice... thank you.READ MOREYou should know Answers to your question are meant to provide general health information but should not replace medical advice you receive from a doctor. No answers should be viewed as a diagnosis or recommended treatment for a condition. Content posted by community members does not necessarily reflect the views of Remedy Health Media, which also reserves the right to remove material deemed inappropriate.DISPLAY TEXTLINK URL
Most PopularLiving WithWhat to Eat With Atrial FibrillationLiving WithCelebrities with Atrial Fibrillation


text:
Need an AMH Test in Ozark, AL? Simply order online 24/7 and get a fast, affordable AMH Test at a lab near you (Find More Locations):
What is an AMH Test?
An AMH Test is a lab test that checks the AMH level. Women in Ozark, AL order the AMH Test for screening or as part of fertility treatment.
What does an AMH Test report look like?
For an AMH Test sample report, please click below:
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For current AMH Test prices, please CLICK HERE.
We are delighted to offer an AMH Test in Ozark, AL.
AMH Test in other cities near Ozark, AL:Ariton | Brundidge | Chancellor | Clio | Daleville | Dothan | Enterprise | Fort Rucker | Midland City | New Brockton | Newton | Newville | Pinckard | Skipperville


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NEW YORK TO BERMUDA FOR $259 R/T NON-STOP (winter-summer)
Amazing deal: New York to Bermuda flights roundtrip as low as $259.
New York, USA
Hamilton, Bermuda
New York, USA
AVAILABILITY: lots of tickets
Departures: February 1-3, 5, 10, 17, 24, 26, 29, March (Monday, Wednesday, Friday), April (Fridays), May 3, 7, 10, 11, 18, June 7, 8
Returns: February – March (Monday, Wednesday, Friday), April 4, 5, 26 + (Thursday, Friday), May (Tuesday, Wednesday, Saturday), June (Monday-Friday)
Some tickets may be $269, which is still super amazing for this destination.
United / American Airlines / Delta /JetBlue
We do not sell travel products or services. We just provide you with information (how/where) about flight deals or links to websites, where you can read more about it.
Information is valid at time of publication. Due to a high amount of posts – it is hard to track down all expired offers, so sometimes you may not find the price mentioned in the post. It means that the deal was sold out or supplier (airlines/agency) has changed the conditions or removed the offer. To avoid these frustrating situations, you can subscribe to our newsletter, Facebook, Twitter, Tumbler.
Image Credits:kansasphoto used under CC BY 2.0 license / Resized from original.


text:
ACLU-NCA Upcoming Events
Expand Messages
- Thursday, June 9th, Noon to 9:00pm, West Lawn of the U.S. Capitol
"Statehood for DC 'Teach In'"
There will be information tables (contact Beverly Miller at beverly@... if you can volunteer), testimonials and a panel discussion in the early evening, followed by a concert and candlelight vigil. Please stop by sometime during the event to help send a message to Congress that it is time to mend the crack in the Liberty Bell and give the citizens of DC representation in Congress.
Saturday, June 11th, 6:30pm Capital Pride, Dupont Circle Neighborhood
(rendezvous @ ACLU-NCA offices 1400 20th Street, NW, #119 5:00pm-5:45pm)
For the 4th year in a row, the ACLU-NCA will "walk with pride" to stand up against anti-gay prejudice and injustice by participating in the Capital Pride parade and festival. The ACLU's participates to bring the gay and straight communities together through a shared interest in equality for all.
I hope you can join the ACLU-NCA for one or both of these events. Be sure to wear your UDC Law/ACLU t-shirt!
Your message has been successfully submitted and would be delivered to recipients shortly.


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This single piece Debrisgaiter from Inov-8, is designed to wrap around a sock in order to prevent dirt and grit from entering the shoe when running.
With Pre-Order, you can purchase the latest items added to our store in advance of them arriving with us.
You Pre-Order is prioritised (at no additional charge); meaning that, as soon as the product arrives with us, we can wrap it straight back up and get it sent out to you - it often never even hits the stock room shelves!
With Pre-Launch, the date when we expect to receive the product ourselves is still a little too hazy to be confident in accepting Pre-Orders.
So, instead, you can submit your email address against the item you are interested in and we will email you as soon as stock arrives on the system.
Estimated Delivery Dates are given on Pre-Order and Pre-Launch products. They are for indication purposes only and can change at any time without notice.
The dates we provide are based on indications given by our suppliers. Whenever we get updated information from our suppliers we will endeavour to update the Estimated Delivery Dates provided on product pages.
This single piece Debrisgaiter, from Inov-8, is designed to wrap around a sock in order to prevent dirt, mud and grit from entering the shoe when running. Fast wicking and quick drying, the Debrisgaiter has a full Durable Water Repellancy treatment and so works well to keep water and mud at bay in wet weather.
The Debrisgaiter is perfect for anyone running in mountain terrain, where bog, scree, gravel and mud may all be encountered on the same run. It's low profile design means it won't slow you down or become uncomfortable. It's easy to put on and take off, and will take up very little room in your pack when not in use.
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text:
Effect of Patient Sex, Clinician Sex, and Sex Role on the Diagnosis of Antisocial Personality Disorder: Models of Underpathologizing and Overpathologizing Biases
Crosby, Jeremy Paul
Sprock, June
Bibliographic Citation
Journal of Clinical Psychology 2004 June; 60(6): 583-604
MetadataShow full item record
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An Ethical Framework for Understanding Patients With Antisocial Personality Disorder Who Develop Dementia: Diagnosing and Managing Disorders of Autonomy Workman, Richard H.; Molinari, Victor; Rezabek, Pamela; McCullough, Laurence B.; Khalsa, Devi Kirin; Trivedi, Sandhya; Kunik, Mark E. (1997)
Conducting Randomised Controlled Trials: Finding Better Ways to Explain Research to People With Anti-Social Personality Disorder Who Have Low Literacy Levels Davidson, Kate M; Espie, Carolyn J; Lammie, Claire (2011-10)The involvement of people with anti-social personality disorder (ASPD) in clinical trials is necessary to developing effective treatment. Low literacy level, however, may be a barrier to their understanding and therefore ...
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Samsoe & Samsoe Rubie Sweater
10% Off First Orders: Join our mailing list to receive a code for 10% off your first order over £20.
Free UK Shipping (2-3 Working Days): Free UK shipping on all orders over £15. Free worldwide shipping on all overs over £100. Next Day UK delivery available.
Best Price Guaranteed: Found a better price elsewhere? Drop us a link in the live chat and we'll match it for you.
More products from Samsoe & Samsoe
The Samsoe & Samsoe Rubie long sleeve sweater has been knitted with an open stitch creating an all-over modern lace pattern. The high round neckline and long sleeves are the perfect canvas for this airtex like knit.
- 72% Viscose, 28% Polyester
- All-over perforations
Returns Information:
If you wish to return or exchange this product, you can return it to us within 14 days of purchase making sure the item is in saleable condition and is accompanied by your order receipt.


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Who doesn't love a story about the underdog who somehow rises from the ashes like a Phoenix and then soars to new heights?
Sadly, that can't be Redemptorist's story any more. The Baton Rouge Diocese's decision to close the 67-year-old school June 30 came as a surprise to some, but not to others.
There was a sharp decline in enrollment over the past 10 years along with significant turnover among the coaches and administration. My heart goes out to the students and staff who now must pick up the pieces and move forward.
The heartache felt by the former players and coaches won't go away in a day, a week or a month. It's a pain that may never fully go away.
But the school will. And with it goes another significant piece of history in north Baton Rouge. This is the same area that already lost one iconic school, Istrouma High, just six months ago.
You can make the argument that neither school was what it used to be. That's definitely a fact. Once the school is gone, like it or not, a significant link to the past usually dies right along with it.
Too dramatic you say? Well, I thought former Redemptorist quarterback and longtime coach Guy Mistretta accurately described what he felt accurately as being "like a death in the family."
That goes for those of us not directly involved with the school. It's something like losing an old friend you've lost touch with or that distant relative who used to show old home movies and pictures while telling you stories about your parents when you were a child.
I remember coming to Redemptorist in the early 1980s for a boys basketball playoff game. In those days, all LHSAA playoff games were held at neutral sites. I was excited about covering South Lafourche and Central for Thibodaux's Daily Comet.
But I was just as excited about seeing the Redemptorist gym. Yes, I am a basketball nerd.
Growing up in Kentucky, I became a huge high school basketball fan. Redemptorist had a rich legacy that included great players like Howard Carter and Derrick Taylor who went on to star at LSU. This place, I figured, had to be as close to home as I could get.
That gym, the Neumann Activities Center, was much like it is today. It's not ornate, though the trophy case was loaded with the spoils from past conquests. Of course I knew that it's not the building; it's the players, coaches and teams that make it what it is.
At the time, I had no idea that the Wolves' football stadium was Majella Field or "The Rock." There was no reason to. Football at Redemptorist often struggled. The Wolves were mired in one of the state's longest football losing streaks when I moved to Baton Rouge in 1989-90.
But under Sid Edwards, the Wolves beat the odds. Yes, there was talk of closing or moving the school to the Central area in the early to mid-1990s. Edwards, another alum, one-upped Gerry DiNardo, who vowed to "Bring the Magic Back" to LSU. Edwards and his staff, which included current head coaches Mistretta (Livonia), David Brewerton (Zachary), Neil Weiner (Dunham) and Guy Blanchard (Port Allen), conjured up magic where there hadn't been much.
The result was three state football titles in the previous decade and a host of talented players, the most recent of which are current LSU lineman La'el Collins and former LSU running back Jeremy Hill of the Cincinnati Bengals. There also were state titles won in baseball, girls basketball, volleyball and softball.
Good times. Great athletes. And fabulous memories.
Here's to making sure those memories live forever.


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6 October 2016
Market Tech Holdings Limited
("Market Tech" or the "Company")
Issue of deferred consideration shares and total voting rights
Market Tech (LSE: MKT) announces that an application has been made to the Official List of the UK Listing Authority and to the London Stock Exchange for the admission of 96,339 ordinary shares of 10p each in the Company (the "Ordinary Shares"'). The Ordinary Shares have been issued as deferred consideration pursuant to the acquisition of Stucco Media Limited announced on 7 May 2015. The balance now remaining to be issued as deferred consideration is 674,370 with 96,339 Ordinary Shares expected to be issued every month over the next six months pursuant to the Stucco Media Limited acquisition, with a final tranche of 96,336 shares to be issued on 7 May 2017.
The Ordinary Shares will be issued fully paid and will, upon issue, rank pari passu in all respects with the Company's existing issued shares. It is expected that the admission of the Ordinary Shares will become effective on Friday 7 October 2016.
Accordingly, following admission, the total number of ordinary shares in Market Tech with voting rights will be 470,105,956. This figure may be used by Market Tech shareholders as the denominator for calculations to determine if they have a notifiable interest in Market Tech under the Disclosure and Transparency Rules, or if such interest has changed.
Market Tech
Charles Butler, Chief Executive Officer
David Brown, Chief Financial Officer
c/o FTI Consulting
+44 (0) 20 3727 1000
FTI Consulting
Richard Sunderland
Dido Laurimore
Claire Turvey
Clare Glynn
+44 (0) 20 372 7 1000
About Market Tech
Market Tech combines the iconic Camden Market real estate assets with digital technology to deliver a living, working, retail and leisure environment. The Company owns approximately 15 acres of real estate assets in Camden, including the Stables Market; Union Street Market, (also known as Buck Street Market); Camden Lock Market; and Hawley Wharf, (formerly known as Camden Lock Village). It also owns separate real estate assets on Camden High Street; Kentish Town Road; properties on Jamestown Road, (including the Camden Wharf Building); The Interchange Building on Oval Road, Utopia Village in Primrose Hill, 1-11 Hawley Crescent and 49 Chalk Farm Road.
The Company owns three e-commerce businesses, referred to as Market Tech Digital. These are Stucco Media, an e-commerce marketing platform, Glispa, a Berlin-based mobile marketing business and Fiver, a B2C online fashion retailer.
- Ends -
This information is provided by RNS


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Monday, March 21, 2011
GENBAND Inks Two Business Deals
Frisco-based telecom gear developer GENBAND has inked two deals today, saying that it has linked its products with the MobileMax Unified Communications Suite, and also linked with another firm on European IPv6 support. MobileMax is a provider of unified communications products, with the integration offering up instant messaging and SMS text support, among other features. Separately, GENBAND said it has linked with Interoute, which operates a voice and data network in Europe, to provide IPv6 support for that company. Financial details of the two deals were not announced.


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How to Use the user interface in Microsoft SharePoint 2010
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You're busy—we'll come to you!
Photo by Vanessa Mendez—vanessamendezphotography.com
Since 1992, Midwifery Today has been a leader in education for midwives. In addition to publishing a magazine, we have put on over 75 conferences throughout the world. In keeping with the times, we are expanding our reach by offering online classes for beginning and seasoned midwives, as well as other birth professionals. Whether you live in Fiji, France or a country in between, we will have the class for you. Check our site for current classes, and look for upcoming specially-themed conferences.
We are using Adobe Connect, an online platform that is robust with features that allow for interaction not only with the speaker but also with other participants. Midwifery Today creates each online class to be fun, informative and inviting. As a participant, you will have the ability to ask questions, participate in activities, interact in breakout sessions, download handouts and surf the links suggested by presenters. Moreover, each class is recorded, so, if you should miss a class, you are able to access the recording at your convenience. Finally, the classes are monitored by a Midwifery Today online event manager who is there to help should any technical difficulties occur.
New Classes
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Past Classes
Past classes are available for purchase.
- Becoming a Midwife
Jan Tritten
Are you interested in a career in birth and don't know where to start? This class is perfect for setting you on the right path to begin a new career in midwifery. Learn what it all entails, what to expect and how to get educated.
- Becoming a Midwife: Keeping Pregnancy Normal
Gail Hart
Learn how to facilitate a normal pregnancy naturally with little to no intervention. This class is a fantastic foundation-builder for aspiring midwives, doulas as well as seasoned midwives and expecting mothers.


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Available to individuals with special interest or need to review or refine selected topics, skills, or experience in the image management field. Additional video presentation and evaluation time available.
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See how Judith Rasband and Conselle bust popular image myths.Read More
Read the Conselle blog for a daily dose of image information!Blog


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An off-campus bookstore was robbed yesterday by a team of people who distracted employees and got money out of a safe in an office off the sales floor. Shoppers were able to give excellent descriptions of the assailents.
Those who witnessed the robbery said the group looked like gypsies, Durrette added. -The Cavalier Daily, 8/24/2005If anyone knows what gypsies look like, let me know. The only image I'm getting is Esmeralda from The Hunchback of Notre Dame.


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C57Bl/6 N mice on a western diet display reduced intestinal and hepatic cholesterol levels despite a plasma hypercholesterolemia
Small intestine and liver greatly contribute to whole body lipid, cholesterol and phospholipid metabolism but to which extent cholesterol and phospholipid handling in these tissues is affected by high fat Western-style obesogenic diets remains to be determined.
We therefore measured cholesterol and phospholipid concentration in intestine and liver and quantified fecal neutral sterol and bile acid excretion in C57Bl/6 N mice fed for 12 weeks either a cholesterol-free high carbohydrate control diet or a high fat Western diet containing 0.03% (w/w) cholesterol. To identify the underlying mechanisms of dietary adaptations in intestine and liver, changes in gene expression were assessed by microarray and qPCR profiling, respectively.
Mice on Western diet showed increased plasma cholesterol levels, associated with the higher dietary cholesterol supply, yet, significantly reduced cholesterol levels were found in intestine and liver. Transcript profiling revealed evidence that expression of numerous genes involved in cholesterol synthesis and uptake via LDL, but also in phospholipid metabolism, underwent compensatory regulations in both tissues. Alterations in glycerophospholipid metabolism were confirmed at the metabolite level by phospolipid profiling via mass spectrometry.
Our findings suggest that intestine and liver react to a high dietary fat intake by an activation of
Obesity is an underlying risk factor in the development of cardiovascular diseases and is frequently associated with hypercholesterolemia and dyslipidemia
Whereas liver, endothelium and adipose tissue have been extensively studied in the context of hypercholesterolemia, dyslipidemia and cardiovascular diseases, the small intestine has long been neglected. A high dietary intake of fat via a Western-style diet requires the epithelium of the upper small intestine to digest and absorb large quantities of dietary TG, sterols and phospholipids (PL)
As hypercaloric diets usually provide large quantities of fat
Since cholesterol and phospholipids are essential components of chylomicron assembly and since intestinal lipoprotein secretion is increased upon high fat feeding, more cholesterol and phospholipids are needed for the epithelial processing of fat, which may cause metabolic adaptations on mRNA levels of genes involved in these pathways. Although the effects of a high fat diet on cholesterol transporter gene expression in mice have already been described in a previous study
Ethics statement
All procedures applied throughout this study were conducted according to the German guidelines for animal care and approved by the Bavarian state ethics committee (Regierung von Oberbayern) according to §8 Abs.1 Tierschutzgesetz under the reference number 209.1/211-2531-41/03.
Animals and sample collection
Conventionally raised eight-week-old male C57Bl/6NCrl mice (Charles River Laboratories) were housed individually in a light- and temperature-controlled facility (lights on 7
Table 1
Diet composition
Western diet
GE (MJ/kg)
ME (MJ/kg)
% protein
% fat
% carbohydrates
Corn starch mod.
Vitamin premix
Mineral/trace elements
Choline chloride
Salt (NaCl)
Beef tallow (premier jus)
Soybean oil
From days 4 to 11, 46 to 53 and 74 to 81, feces produced by five mice of each group were collected, dried at 50°C to constant weight and ground. Gross energy was determined using an isoperibol bomb calorimeter (model number 6300, Parr Instrument GmbH), with benzoic acid used as a standard.
After 12 weeks, mice in a non-fasting state were anesthetized using isoflurane and blood was collected from the retro-orbital sinus. Mice were then killed by cervical dislocation. Tissues were harvested at the same time of the light period (between 9 and 12
Glucose tolerance test
After 9 weeks of feeding, mice were subjected to a glucose tolerance test. After 14 hours of food deprivation, mice were injected with a 20% glucose solution (B. Braun Melsungen AG) intraperitoneally (10 ml/kg of body weight) and blood glucose was measured from the tail vein 0, 15, 30, 60 and 120 minutes after the injection using an Accu-Check blood glucose meter (Roche Diagnostics).
Serum and tissue analysis
Serum cholesterol, glucose, HDL cholesterol and TG were determined using Piccolo® Lipid Panel Plus Reagent Discs and a Piccolo Blood Chemistry Analyzer (Hitado Diagnostic Systems). Serum insulin was determined using an Ultra Sensitive Mouse Insulin ELISA kit (Crystal Chem Inc.), according to the manufacturer's instructions. Inter- and intra-assay CV were generally ≤ 10%.
For determination of hepatic and intestinal TG and PL concentration, tissues were ground in liquid nitrogen and dissolved in 0.9% NaCl. Samples were centrifuged for 10 min at 10 000 g and PL concentration was determined using a commercial enzymatic colorimetric kit, following the manufacturer's instructions (Phospholipids C, Wako Chemicals GmbH). TG were extracted from the samples as follows: after centrifugation (10 min, 10 000 g), supernatants were incubated in alcoholic KOH (30 min, 70°C), 0.15 mol/l magnesium sulfate was added to the solution and after centrifugation (10 min, 10 000 g), TG concentration was determined using a commercial enzymatic colorimetric kit, following the manufacturer's instructions (Triglycerides liquicolormono, Human GmbH). Hepatic and intestinal cholesterol concentration was determined using a commercial enzymatic colorimetric kit, following the manufacturer's instructions (Cholesterol/Cholesteryl Ester Quantitation Kit, Biocat GmbH).
For determination of hepatic and intestinal acylcarnitine, phosphatidylcholine (PC) and sphingolipid concentration, tissues were ground in liquid nitrogen and analytes were extracted using 60 μl MeOH per 10 mg homogenized tissue. Samples were vortexed, centrifuged and the assay was performed in 10 μl of the supernatant using the
Fecal neutral sterol and bile acids determination
Sterol analysis in fecal samples was performed as previously described
RNA isolation
Total RNA from the upper and lower small intestine and from the liver was isolated using Trizol reagent (Invitrogen) until the ethanol precipitation step and further purified using the QIAGEN RNeasy Mini Kit spin columns (QIAGEN GmbH). RNA concentration and purity were measured on a NanoDrop ND-1000 UV-vis spectrophotometer (NanoDrop Technologies).
Gene Chip expression array hybridization
Total RNA was reverse-transcribed and the corresponding cRNA was biotinylated and fragmented following the original protocol of Affymetrix (Affymetrix Inc.). For each experimental group, 6 biological replicates were hybridized overnight on The Gene Chip® 3' Expression Arrays (Affymetrix), customized for NuGO (The European Nutrigenomics Organization). A more detailed description of the platform can be found on the Gene Expression Omnibus, accession number GPL7441. The arrays were then washed and scanned following the instructions of the provider. A total of 24 arrays were hybridized. Detailed methods for the labeling and subsequent hybridizations to the arrays are provided in the eukaryotic section of the GeneChip Expression Analysis Technical Manual from Affymetrix.
Transcriptome data analysis and statistics
The quality of the data was analyzed by a Bioconductor
Overrepresentation of gene ontology (GO) Biological Process subsets was made using an ErmineJ overrepresentation analysis
Heat map diagrams displaying standard scores of signal intensities of selected genes were made using the Genesis software
cDNA synthesis and real-time quantitative PCR
For each liver sample, 10 ng of isolated total RNA were used for real-time quantitative PCR (qPCR) using the QuantiTect® SYBR Green RT-PCR kit (Qiagen GmbH) on a Mastercycler ep realplex apparatus (Eppendorf), following the suppliers' protocols. Gene sequences were retrieved from the database Ensembl
Additional file 1
Table S1. Primer sequences.
Click here for file
Statistical analysis
For all groups, data were expressed as mean ± SEM. Statistical analyses were performed using the Prism 4 software (GraphPad Software). Prior to Student's t-test, data were tested for normal distribution and equality of variances. In the case of inhomogeneous variances, Welch's correction was applied to Student's t-test. Differences in weight gain, food and water intake, digested energy, fecal neutral sterol and bile acids output and daily sterol balance over the feeding period were tested by using the MIXED procedure in SAS (Version 9.2; SAS Institute Inc.) with time as a repeated factor
Western diet feeding led to obesity, hyperglycemia, hyperinsulinemia and elevated blood cholesterol levels
After 12 weeks on a Western diet, mice presented the expected hallmarks of obesity. Data on final body weight, as well as cumulative food, energy, water and macronutrient intake is provided in Additional file
Additional file 2
Table S2. Effect of a chronic Western diet on final body weight, cumulative food, energy, water, macronutrient and cholesterol intake and energy assimilation.
Click here for file
Additional file 3
Figure S1. Body weight development in mice fed the different diets. Symbols: black diamonds, control diet; grey squares, Western diet. Data are presented as mean ± SEM (n = 12). **
Click here for file
Additional file 4
Figure S2. Glucose tolerance in mice fed the different diets for 9 weeks. A: A glucose tolerance test was carried out after 9 weeks of dietary intervention following 14 h of food deprivation. Blood was collected from the tail vein 0, 15, 30, 60 and 120 min after an intraperitoneal 20% glucose solution injection (10 ml/kg of body weight) and blood glucose was measured. Symbols: black diamonds, control diet; grey squares, Western diet. B: Area Under the Curve (AUC) calculated from the glucose tolerance test. Black bar: control diet; grey bar: Western diet. Data are presented as mean ± SEM (n = 12). *
Click here for file
Additional file 5
Table S3. Effect of a chronic Western diet on selected blood parameters.
Click here for file
Mice on Western diet displayed increased fecal neutral sterol content
Feces from five mice per group were collected from days 4 to 11, 46 to 53 and 74 to 81 and analyzed for neutral sterol and bile acids content. Mice receiving the Western diet exhibited an increase in fecal neutral sterol output (Figure
Figure 1
Sterol balance data obtained from mice fed the different diets for 12 weeks
Sterol balance data obtained from mice fed the different diets for 12 weeks. Feces were collected at three time points during the feeding trial and neutral sterol and bile acids content was measured by gas chromatography-mass spectrometry A: Daily fecal neutral sterol output. B: Daily fecal bile acids output. C: Daily sterol balance measured by subtracting fecal neutral sterol and bile acids output from cholesterol intake. Symbols: black diamonds, control diet; grey squares, Western diet. Data are presented as mean ± SEM (n = 5). **
This balance did not include beta muricholic acid and steroid hormones derivatives. Interestingly, mice fed the Western diet displayed a negative sterol balance, losing between 0.50 to 0.75 μmol of cholesterol per day. Mice fed the control diet showed an even more pronounced negative sterol balance, excreting between 1.45 to 1.65 μmol of cholesterol per day, although this was only significantly different from mice fed the Western diet between days 46 and 53 (Figure
Obese mice displayed decreased intestinal and hepatic cholesterol levels
Despite a much greater dietary cholesterol intake (Additional file
Figure 2
Cholesterol, TG and PL content in intestine and liver of mice fed the different diets for 12 weeks
Cholesterol, TG and PL content in intestine and liver of mice fed the different diets for 12 weeks. A: Cholesterol concentration in the upper small intestine (n = 5). B: Cholesterol concentration in the liver (n = 12). C: TG concentration in the upper small intestine (n = 5-6). D: TG concentration in the liver (n = 11-12). E: PL concentration in the upper small intestine (n = 5-6). F: PL concentration in the liver (n = 11-12). Control diet: black bar; Western diet: grey bar. Data are presented as mean ± SEM. *
Cholesterol transporter genes showed reduced expression levels while cholesterol synthesis genes showed increased expression levels in the small intestine of obese mice
Expression levels of genes encoding proteins directly involved in cholesterol transport or metabolism in the small intestine, obtained from microarray analysis, are shown in Table
Table 2
Effect of a chronic Western diet on the expression of genes related to cholesterol and lipid metabolism in the small intestine
Gene name
ATP-binding cassette, sub-family A, 1
ATP-binding cassette, sub-family G, 5
ATP-binding cassette, sub-family G, 8
acetyl-Coenzyme A acyltransferase 2
Apolipoprotein A-II
Apolipoprotein C-II
Caveolin 1
CD36 antigen
Carboxylesterase 1D
< 0.001
Carboxylesterase 1G
< 0.001
Carnitine palmitoyltransferase 1a, liver
Cytochrome P450, family 27, subfamily a, polypeptide 1
Cytochrome P450, family 51
7-dehydrocholesterol reductase
3-hydroxy-3-methylglutaryl-Coenzyme A lyase
3-hydroxy-3-methylglutaryl-Coenzyme A reductase
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
< 0.001
Hydroxysteroid (17-beta) dehydrogenase 7
Isocitrate dehydrogenase 1 (NADP+), soluble
LDL receptor
< 0.001
Liver × receptor alpha
Liver × receptor beta
Malic enzyme 1, NADP(+)-dependent, cytosolic
< 0.001
Microsomal triglyceride transfer protein
Mevalonate decarboxylase
Mevalonate kinase
Niemann-Pick C1-like protein 1
< 0.001
NAD(P) dependent steroid dehydrogenase-like
Proprotein convertase subtilisin/kexin type 9
Phosphomevalonate kinase
< 0.001
Scavenger receptor class B, member 1
Stearoyl-Coenzyme A desaturase 1
< 0.001
Stearoyl-Coenzyme A desaturase 2
< 0.001
Solute carrier family 25 (mitochondrial carrier, citrate transporter), member 1
< 0.001
solute carrier family 27 (fatty acid transporter), member 4
Squalene epoxidase
Sterol regulatory element binding factor 2
Transmembrane 7 superfamily member 2
Figure 3
Heat map diagrams of differentially expressed genes in the small intestine upon Western diet feeding
Heat map diagrams of differentially expressed genes in the small intestine upon Western diet feeding. A: Standard scores of differentially expressed genes related to cholesterol metabolism (GO Biological Processes: cholesterol metabolic process, cholesterol biosynthetic process, cholesterol transport, cholesterol homeostasis, positive regulation of cholesterol efflux, regulation of cholesterol efflux, cholesterol efflux, regulation of cholesterol metabolic process, regulation of cholesterol storage, regulation of cholesterol biosynthetic process, reverse cholesterol transport). B: Standard scores of differentially expressed genes related to PL metabolism (GO Biological Processes: PL metabolic process, PL biosynthetic process, PL catabolic process, PL efflux, PL transport). Capital letters indicate: C, control; W, Western diet. Differentially expressed genes with a q-value ≤ 0.05 were included in the analysis. Green and red indicate down- and up-regulation of gene expression, respectively.
Additional file 6
Table S4. Effect of a chronic Western diet on gene expression in the upper small intestine. Data are presented by ascending q-values. Abbreviations: FC: fold change.
Click here for file
Additional file 7
Table S5. Effect of a chronic Western diet on gene expression in the lower small intestine. Data are presented by ascending q-values. Abbreviations: FC: fold change.
Click here for file
Additional file 8
Table S6. Effect of a chronic Western diet on GO Biological Process subsets in the upper small intestine. Data are presented by ascending corrected p-value.
Click here for file
To assess whether these changes were restricted to intestinal tissue or similarly occur in the liver, we used qPCR to determine transcript levels of the preselected target genes involved in cholesterol metabolism (Table
Table 3
Effect of a chronic Western diet on the expression of genes related to cholesterol metabolism in the liver
Gene name
Cytochrome P450, family 51
1.80 ± 0.28
7-dehydrocholesterol reductase
1.72 ± 0.23
3-hydroxy-3-methylglutaryl-Coenzyme A reductase
2.23 ± 0.48
Phosphomevalonate kinase
1.22 ± 0.16
Sterol regulatory element binding factor 2
1.37 ± 0.08
Liver and small intestine exhibited changes in phospholipid status and metabolism
Significant changes in expression levels of genes encoding proteins directly involved in PL processing in the small intestine are shown in Table
Table 4
Effect of a chronic Western diet on the expression of genes related to phospholipid metabolism (based on GO classification) in the small intestine
Gene name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase)
CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
Choline/ethanolaminephosphotransferase 1
Choline kinase alpha
Lysophosphatidylcholine acyltransferase 3
Lysophosphatidylglycerol acyltransferase 1
< 0.001
Phospholipid scramblase 2
< 0.001
Phospholipid scramblase 4
< 0.001
Phosphatidic acid phosphatase type 2B
Based on LC-MS/MS analysis, a variety of changes in intestinal and hepatic phospholipids were identified. The fold changes of significantly regulated phosphatidylcholine (PC) species in tissue samples of mice fed the Western diet compared to mice fed the control diet are displayed in Figure
Figure 4
Effect of a chronic Western diet on the level of phosphatidylcholine species
Effect of a chronic Western diet on the level of phosphatidylcholine species. Hepatic and intestinal PC levels were detected using LC-MS/MS. A: Significantly regulated PC in the small intestine (n = 6). B: Significantly regulated PC in the liver (n = 6). Data are presented as fold change (Western diet versus control) ± SEM. Abbreviations: PC.aa.: phosphatidylcholine diacyl; PC.ae.: phosphatidylcholine acyl-alkyl; lysoPC.a.: lysophosphatidylcholine acyl.
Additional file 9
Table S7. Effect of a chronic Western diet on the level of selected phospholipids (PL). Hepatic and intestinal PC levels were detected using LC-MS/MS. Data are presented are mean in μmol/g of protein ± SEM. Abbreviations: C0: DL-carnitine; C2: acetyl-L-carnitine; C3: propionyl-L-carnitine; C4: butyryl-L-carnitine; C4.OH..C3.DC.: hydroxybutyryl-L-carnitine (malonyl-L-carnitine); C5.DC..C6.OH.: glutaryl-L-carnitine (hydroxyhexanoyl-L-carnitine); C5.OH..C3.DC.M.: hydroxyvaleryl-/-isovaleryl-/- methylbutyryl-L-carnitine (methylmalonyl-L-carnitine); C6:1: hexenoyl-L-carnitine; C7-DC: pimelyl-L-carnitine; C16-OH: hydroxyhexadecanoyl-L-carnitine; C16:2: hexadecadienyl-Lcarnitine; C18:1: octadecenoyl-L-carnitine; C18:1-OH: hydroxyoctadecenoyl-L-carnitine; C18:2: octadecadienyl-L-carnitine; FC: fold change; H1: hexose; lysoPC.a.: lysophosphatidylcholine acyl; n.d.: not determined; PC.aa.: phosphatidylcholine diacyl; PC.ae.: phosphatidylcholine acyl-alkyl; SM: sphingomyelin; SM..OH..: hydroxysphingomyeline.
Click here for file
The main finding of this study is that obese mice fed a Western-style high fat diet containing cholesterol displayed reduced cholesterol levels in intestine and liver, despite a plasma hypercholesterolemia, when compared to mice given a cholesterol-free high carbohydrate diet. Not only did the mice on the Western diet exhibit phenotypic changes towards a metabolic syndrome, such as impaired glucose clearance, but also major adaptive changes in cholesterol and phospholipid metabolism.
Proper fat digestion and absorption in the small intestine requires luminal bile acids and PL for formation of micelles. Incorporation of TG into CM after reassembly in the enterocytes also requires large quantities of PL and cholesterol. Chronic high fat feeding consequently increases the needs of the small intestine for additional cholesterol, PL and bile acids for processing and secretion of the fat into circulation. Although Western diets based on animal lipid sources provide extra cholesterol, this did not seem to be sufficient to meet the increased demands of the intestine. Based on the microarray data, we provide evidence that the subsequent fall in tissue cholesterol levels may initiate changes in gene expression that can be interpreted as an increase in
Figure 5
Effect of a chronic Western diet on the expression of genes related to cholesterol metabolism in the small intestine
Effect of a chronic Western diet on the expression of genes related to cholesterol metabolism in the small intestine. Fold changes are displayed next to differentially expressed genes with color code provided. Red squares indicate upregulated genes; green squares indicate downregulated genes. Cholesterol concentration was found to be decreased whereas TG concentration was found to be increased in mice receiving the Western diet. Abbreviations and fold changes are listed in Table 2.
Evidence for an increased
Genes involved in the cholesterol biosynthesis pathway are primarily under the control of the membrane-bound transcription factor sterol regulatory element-binding protein 2 (Srebp-2)
Taken together, the changes in mRNA levels observed in the mice receiving a cholesterol-containing Western diet may be interpreted as an adaptive response to meet an increased cholesterol demand of the intestine for proper handling of large quantities of fat and for CM formation. The changes suggest a reduced luminal efflux of cholesterol, an increased LDL-receptor mediated reverse uptake, a reduced breakdown and an enhanced
Lipid and cholesterol handling, both in the intestinal lumen and for transport via CM and other lipoproteins, requires PL. Numerous genes involved in PL homeostasis exhibited altered expression levels in the small intestine of the obese mice. For example, genes encoding phospholipid scramblases 2 and 4 were upregulated 7.8- and 24.3-fold respectively in mice fed the Western diet. Phospholipid scramblases represent a group of homologous ATP-independent bidirectional lipid translocators, involved in generation and maintenance of lipid asymmetry in the plasma membrane and are conserved in all eukaryotes
Analysis of total PL content in liver revealed significantly reduced levels in mice fed the Western diet, whereas changes in the small intestine did not reach significance. However, as demonstrated by Hicks et al., different tissues present unique PL signatures
Furthermore, liver also exhibited alterations in PC species with longer carbon chains (C38 and C40). In essentially all mammalian cells, PC is synthesized almost exclusively through the CDP-choline pathway
In summary, in addition to obesity, impaired glucose tolerance and hepatic steatosis, a Western-style high fat diet, which usually contains larger quantities of cholesterol, also causes changes in enterohepatic cholesterol and PL status in C57Bl/6 N mice. Despite a higher dietary intake of cholesterol and increased serum cholesterol levels, tissue cholesterol levels in small intestine and liver were reduced when compared to lean mice fed a cholesterol-free high carbohydrate diet. Changes in gene expression suggest that the increased cholesterol demand of tissues for fat absorption and transport may be met by a) an increased export of acetyl-CoA from mitochondria and increased cytosolic cholesterol synthesis, b) a reduced breakdown of cholesterol, c) an increased reverse uptake of cholesterol via LDL and d) a reduced back-flux of cholesterol into the intestinal lumen. In addition, we demonstrate that intestine and liver show major changes in gene expression and in the levels of selected PC species, indicative for a) an increased PC synthesis via the CDPcholine pathway, b) an increased PE methylation pathway activity in the liver and c) alterations in membrane PL remodeling. The observed cholesterol paradox and alterations in PL metabolism call for more studies to identify the underlying molecular mechanisms by which Western-style high fat diets cause these changes.
ABCG5: ATP-binding cassette sub-family G member 5; ABCG8: ATP-binding cassette subfamily G member 8; AR(1): autoregressive order one; ARH(1): heterogeneous autoregressive order one; ARMA(1,1): autoregressive moving average order one; CA: cholic acid; CM: chylomicron; CSH: heterogeneous compound symmetry; DCA: deoxycholic acid; GO: gene ontology; Lpact3: Lysophosphatidylcholine acyltransferase 3; MCA: muricholic acid; MUFA: mono unsaturated fatty acids; NPC1L1: Niemann-Pick C1 Like Protein 1; PC: phosphatidylcholine; PC.aa.34:1: phosphatidylcholine diacyl 34:1; PC.ae.34:1: phosphatidylcholine acyl-alkyl 34:1; PE: phosphatidylethanolamine; PL: phospholipid; PPAR-α: peroxisome proliferator-activated receptor alpha; qPCR: real-time quantitative Polymerase Chain Reaction; TG: triacylglyceride; TICE: trans-intestinal cholesterol excretion; W: Western diet.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
CDe carried out the animal experiment, the transcriptomics study, analyzed data and drafted the manuscript; CDa carried out the qPCR; MS carried out the metabolomics study; SK and GJ carried out the fecal neutral sterol and bile acids determination; HD designed the study and drafted the manuscript. All authors read and approved the final manuscript.
The authors would like to thank Adelmar Stamfort for his help in statistical analysis, Elmar Jocham, Ronny Scheundel, Johanna Welzhofer and Sabine Mocek for technical assistance.
CDe is funded by the EU FP6 project
Obesity, metabolic syndrome, and cardiovascular disease
Hypertriglyceridemia and elevated lipoprotein(a) are major risk factors for coronary events in middle-aged men
Hypertriglyceridemia as a cardiovascular risk factor
Mechanisms linking obesity with cardiovascular disease
Intestinal lipid absorption
CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice
Identification of the major intestinal fatty acid transport protein
Fatty acid transport protein 4 is dispensable for intestinal lipid absorption in mice
Intestinal lipoprotein overproduction in insulin-resistant states
Niemann-Pick C1 Like 1 Protein Is Critical for Intestinal Cholesterol Absorption
Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis
The target of ezetimibe is Niemann-Pick C1Like 1 (NPC1L1)
Influence of class B scavenger receptors on cholesterol flux across the brush border membrane and intestinal absorption
The ABCG5 ABCG8 sterol transporter and phytosterols: implications for cardiometabolic disease
De novo cholesterol synthesis in three different animal models of diabetes
Bile acids as regulatory molecules
Dietary phospholipids, hepatic lipid metabolism and cardiovascular disease
Dietary fat intake does affect obesity!
Adaptive changes of the rat small intestine in response to a high fat diet
Chronic high-fat diet affects intestinal fat absorption and postprandial triglyceride levels in the mouse
The role of the small intestine in the development of dietary fat-induced obesity and insulin resistance in C57BL/6 J mice
Increased dietary triacylglycerol markedly enhances the ability of isolated rabbit enterocytes to secrete chylomicrons: an effect related to dietary fatty acid composition
Short-term adaptation of postprandial lipoprotein secretion and intestinal gene expression to a high-fat diet
Intestinal lipid transport and chylomicron production: Possible links to exacerbated atherogenesis in a rodent model of the metabolic syndrome
Differential regulation of intestinal lipid metabolism-related genes in obesity-resistant A/J vs. obesity-prone C57BL/6J mice
Lipoapoptosis: its mechanism and its diseases
High saturated-fat diet induces apoptosis in rat enterocytes and blunts GIP and insulin-secretive response to oral glucose load
A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine
Metabolomics of prolonged fasting in humans reveals new catabolic markers
Nanoscale and customary non-esterified sitosterols are equally enriched in different body compartments of the guinea pig
Determination of underivatised sterols and bile acid trimethyl silyl ether methyl esters by gas chromatography-mass spectrometry-single ion monitoring in faeces
Bioconductor: open software development for computational biology and bioinformatics
MADMAX - Management and analysis database for multiple omics experiments
A Model-Based Background Adjustment for Oligonucleotide Expression Arrays
Linear models and empirical bayes methods for assessing differential expression in microarray experiments
Gene function analysis in complex data sets using ErmineJ
Genesis: cluster analysis of microarray data
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
Statistical analysis of repeated measures data using SAS procedures
NSDHL, an enzyme involved in cholesterol biosynthesis, traffics through the Golgi and accumulates on ER membranes and on the surface of lipid droplets
Sterol dependent regulation of human TM7SF2 gene expression: Role of the encoded 3[beta]-hydroxysterol [Delta]14-reductase in human cholesterol biosynthesis
Disruption of the gene encoding 3beta-hydroxysterol Delta-reductase (Tm7sf2) in mice does not impair cholesterol biosynthesis
Luminal lipid regulates CD36 levels and downstream signaling to stimulate chylomicron synthesis
Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes
The SREBP pathway-insights from Insigs and insects
The Low-Density Lipoprotein Pathway and its Relation to Atherosclerosis
Activation of the liver × receptor stimulates trans-intestinal excretion of plasma cholesterol
Regulation of direct transintestinal cholesterol excretion in mice
Role of stearoyl-coenzyme A desaturase in regulating lipid metabolism
The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1
Human liver cholesteryl ester hydrolase: cloning, molecular characterization, and role in cellular cholesterol homeostasis
Dietary fructose and glucose differentially affect lipid and glucose homeostasis
Phospholipid scramblases: an overview
Phospholipid scramblase: an update
Intestinal lipoprotein assembly
Intestinal absorption of long-chain fatty acids: Evidence and uncertainties
Unique molecular signatures of glycerophospholipid species in different rat tissues analyzed by tandem mass spectrometry
Thematic Review Series: Glycerolipids. Phosphatidylcholine and choline homeostasis
The methylation of phosphatidylethanolamine
Molecular distinction of phosphatidylcholine synthesis between the CDP-choline pathway and phosphatidylethanolamine methylation pathway
Identification of a physiologically relevant endogenous ligand for PPARalpha in liver
Peroxisome proliferator-activated receptor alpha target genes
Discovery of a lysophospholipid acyltransferase family essential for membrane asymmetry and diversity


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text: In the end he became best known for off-field events. He once had his head shaved by Devon Malcolm in the West Indies and missed a Test with sunstroke.He also once failed to turn up on time for a Test, claiming a burst tyre, an excuse that cut no ice with then chairman of selectors Ray Illingworth.In the summer of 1998 he angered many of his former teammmates by indirectly implicating England players in the match-fixing affair although he never revealed a specific name in public. Police investigated his claims but took no further action.Copyright AFP 2000


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(Copyright 2012 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)


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