text:
By Alex Byington
Killeen Daily Herald
BELTON – Emily Leib was exhausted.
She had just completed her 15th inning in a matter of three hours and didn't want to toe the rubber again.
So, as she stepped to the plate in the bottom of the eighth with the Lady Hornets deadlocked 0-0 with rival Lorena, Leib took matters into her own hands – and drilled the first pitch she saw over the left-center field wall for a walk-off two-run shot and a 2-0 victory.
"I was (thinking), I don't want to pitch another inning – it was the 15th one (in a row Saturday)," said the exasperated Leib, who grinned from ear-to-ear around the bases before teammates swarmed her at home plate. "It was an inside pitch and I'm really happy I hit one over."
Thanks to Leib's first home run since her freshman season, Gatesville catapulted over Lorena to claim the Division II championship, winning the second annual Cen-Tex Hit-N-Run Classic for the second consecutive season.
"It felt really good because we weren't hitting the ball. Well we were, but we were just kind of chipping away at it, so it felt good to just kind of finish it," Leib said.
Both Gatesville (13-4) and No. 15 Lorena finished division play tied with a 5-1 record, but the Lady Hornets took home the title because of the two-run differential against the Lady Leopards was greater than any of Lorena's single-run victories.
"It's huge, confidence-wise," said Gatesville coach Michelle Edwards. "It's a young team with a new mixture of girls, and it helps out with confidence in each other and confidence that they can come out with some big wins."
While Leib got it done with her bat on Saturday, it was her consistent right arm that carried Gatesville to its five wins – the lone defeat being a 6-2 loss to Lampasas on Friday while Leib rested after pitching all eight innings of a 4-3 victory over Wolfforth Frenship the game before.
"You've got to give (Leib) a lot of credit because she brings a lot of poise on the mound, and if you watch her, she never changes her facial expressions and she's always ready to go," said Edwards. "She's the type of kid that wants the ball in her hands at all times and she does well under pressure."
Throwing 35 innings – which included a pair of back-to-back games – spanning the three days, Leib struck out 57 batters for an average of 11.4 per contest while allowing just three earned runs the entire tournament.
Gatesville 4, Liberty Hill 2 – With three runs in the bottom of the sixth inning, after opening the frame with three consecutive singles, the Lady Hornets made amends for two errors that led to two unearned runs in the top of the fourth.
Gatesville's Jaycee Summers and Leib opened the sixth with back to back singles, cleanup hitter Shelby Watson knocked in Summers on a single of her own while Leib scored on a sacrifice from Amber Colson in the following at bat. Watson scored two batters later on a fielder's choice by Paige Chambers.
Lampasas 7, Brownwood 6, 9 inn. – Hadley Brown had a walk-off, two-run single in the bottom of the ninth inning, and the Lady Badgers evened up their tournament record at 3-3 for the weekend.
Knotted at 5-5 entering the top of the ninth after both teams scored two runs in the first inning of the international tie breaker, Lampasas loaded the bases with a bunt and a walk before Brown came through with the heroics.
Kallen Vann finished 3-for-5 while Tealey Farquhar, Marissa Bogart and Madison Morris each added RBI singles.
Frenship 4, Lampasas 0 – Limited to just three hits, the Lady Badgers were never able to get much going offensively as Frenship took advantage of a spot start from Lampasas' No. 2 pitcher Kady Crow.
Magnolia 5, Belton 1 – One game after exploding for 11 runs, the Lady Tigers' hitting woes re-emerged as Magnolia touched up starter Ashley Cook for five runs combined over the fifth and sixth innings.
Following an inning-opening error on a pop fly to shallow center field in the bottom of the fifth, the Lady Bulldogs scored on three straight RBI singles by Victoria Birdwell, Katy Adar and Ariel Hovis to take a 3-1 lead.
Contact Alex Byington at email@example.com or (254) 501-7566.


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Chronic treatment with agomelatine or venlafaxine reduces depolarization-evoked glutamate release from hippocampal synaptosomes
Cellular and molecular mechanisms
Growing compelling evidence from clinical and preclinical studies has demonstrated the primary role of alterations of glutamatergic transmission in cortical and limbic areas in the pathophysiology of mood disorders. Chronic antidepressants have been shown to dampen endogenous glutamate release from rat hippocampal synaptic terminals and to prevent the marked increase of glutamate overflow induced by acute behavioral stress in frontal/prefrontal cortex. Agomelatine, a new antidepressant endowed with MT1/MT2 agonist and 5-HT2C serotonergic antagonist properties, has shown efficacy at both preclinical and clinical levels.
Chronic treatment with agomelatine, or with the reference drug venlafaxine, induced a marked decrease of depolarization-evoked endogenous glutamate release from purified hippocampal synaptic terminals in superfusion. No changes were observed in GABA release. This effect was accompanied by reduced accumulation of SNARE protein complexes, the key molecular effector of vesicle docking, priming and fusion at presynaptic membranes.
Our data suggest that the novel antidepressant agomelatine share with other classes of antidepressants the ability to modulate glutamatergic transmission in hippocampus. Its action seems to be mediated by molecular mechanisms located on the presynaptic membrane and related with the size of the vesicle pool ready for release.
Compelling evidence suggest that long-term changes in different brain areas and circuits mediating cognitive and emotional behaviors represent the biological underpinnings of mood and anxiety disorders
We have previously found that chronic treatment with different antidepressants (fluoxetine, desipramine, reboxetine) significantly reduced depolarization-evoked glutamate release from hippocampal synaptic terminals (synaptosomes)
Agomelatine is a new antidepressant, synergically acting as an agonist of MT1/MT2 receptors and as an antagonist of 5-HT2C receptors
Chronic treatment with agomelatine or venlafaxine reduces depolarization-evoked release of glutamate from hippocampal synaptosomes
Synaptosomes were purified from the hippocampus of chronically drug- or vehicle-treated rats, and exposed in superfusion to KCl or ionomycin to assess glutamate and γ-amminobutyric acid (GABA) release. As shown in Figure
Figure 1
Effect of chronic treatment with agomelatine or venlafaxine on depolarization-evoked glutamate and GABA release and on SNARE complex accumulation in hippocampal synaptosomes.
Effect of chronic treatment with agomelatine or venlafaxine on depolarization-evoked glutamate and GABA release and on SNARE complex accumulation in hippocampal synaptosomes. A) 15 mM KCl-evoked glutamate and GABA release and 0.5 mM ionomycin-evoked glutamate release from hippocampal synaptosomes of rats chronically treated with vehicle (CNT), agomelatine (AGO) or venlafaxine (VFX). B) Representative Western Blot of SNARE complexes in hippocampal synaptosomes visualized with a monoclonal antibody for syntaxin-1. The two SNARE complexes at approximately 100 and 80 kDa and syntaxin-1 monomer are indicated by arrows. Each single SNARE complex was normalized on monomeric syntaxin-1 in the same lane. C) Quantitation of syntaxin-1 in CNT and rats chronically treated with VFX or AGO. D) Quantitation of normalized 100 kDa SNARE complex in CNT rats and rats chronically treated with VFX or AGO. E) Quantitation of normalized 80 kDa SNARE complex in CNT rats, and rats chronically treated with VFX or AGO. All data are expressed as mean ± SEM. * p<0.05, ** p<0.01 vs. CNT rats, Newman-Keuls post-hoc tests following one-way ANOVA (n = 6–10 rats/group in duplicate).
Chronic antidepressant treatments reduce SNARE complex in hippocampal presynaptic membranes
The core of the presynaptic SNARE complex is formed by the coiled-coil domain interaction of two membrane proteins (syntaxin-1 and Soluble NSF Attachment Protein-25, SNAP-25) and one vesicle protein (synaptobrevin-2)
Because in hippocampus glutamate synaptic terminals represent the vast majority of total synapses
The analysis of SNARE complexes in purified presynaptic membranes revealed two major syntaxin-1-containing complexes, migrating at ~100 kDa and ~80 kDa (Figure
Overall, the present findings show that chronic administration of agomelatine or the reference antidepressant venlafaxine significantly reduced the endogenous release of glutamate from hippocampal synaptosomes, as previously found with other traditional antidepressants
Discussion and conclusion
This work demonstrates that chronic treatment with the new antidepressant agomelatine, a MT1 and MT2 receptor agonist and 5-HT2C receptor antagonist, as well as with venlafaxine, a selective serotonin and noradrenaline reuptake inhibitor, markedly reduces depolarization-evoked endogenous release of glutamate from hippocampal synaptosomes. Interestingly, the depolarization-evoked release of GABA was not modified, suggesting that both drugs selectively inhibit glutamatergic release without affecting GABA transmission. These results are in line with previous studies, both in vivo and ex vivo, showing similar effects on glutamatergic transmission of a number of traditional antidepressants and of agomelatine (
The reduction of glutamate release with no changes in GABA release suggests an alteration in the balance between excitatory and inhibitory neurotransmission that could improve the signal to noise ratio in glutamate transmission, when it becomes compromised by excessive release. In this regard, we have demonstrated that chronic treatment with different classes of antidepressants, includ-ing agomelatine, was able to completely prevent the marked increase of depolarization-evoked glutamate release from prefrontal and frontal cortex synaptosomes induced by acute stress
Another intriguing result of this study is that, despite chronic agomelatine inhibited glutamate release evoked by 15 mM KCl depolarization, it had no effect on release induced by 0.5 mM ionomycin. Indeed, if it is generally agreed that electrical/chemical depolarization mainly induces the fusion of vesicles of the readily releasable pool (RRP)
Indeed, the SNARE complex and associated proteins play a critical role in vesicle docking, priming, fusion and synchronization of neurotransmitter release at presynaptic membranes and it was established that the SNARE complex corresponds to the minimal machinery for membrane fusion in eukaryotic cells, forming a stable complex that make the vesicles competent for fusion
In conclusion, the present study demonstrated that chronic agomelatine dampens hippocampal glutamate neurotransmission, a likely component of the therapeutic action of antidepressants. The intriguing finding of reduced accumulation of SNARE complexes in presynaptic membranes suggests selected mechanisms in the exocytotic machinery as possible molecular targets of these drugs.
Animals and treatments
Adult male Sprague–Dawley rats (170–200 g) were purchased from Charles River (Calco, Italy). Animals were kept at constant temperature (22°C) with a regular 12 h light/dark cycle (light-off at 7 pm). The rats were housed in groups of four with ad libitum access to food and water. Rats were treated with agomelatine (40 mg/kg i.p.), venlafaxine (10 mg/kg i.p.) or vehicle (hidroxyethylcellulose, 1%, 1 ml/Kg, i.p.) for 21 days. All drugs were administered at 5.00 pm (2 h before the start of the dark cycle, 7 pm). Animals were sacrified 16 h after the last drug administration, and the hippocampus (HPC) was quickly removed.
All animal procedures were conducted according to current regulations for animal experimentation in Italy (Decreto Legislativo 116/1992) and the European Union (European Communities Council Directive 86/609/EEC) and following authorization by the Italian Ministry of Health to the University of Milano for experimental use of animals (Decreto Legislativo 295/2012-A).
Preparation of purified synaptosomes
Purified synaptic terminals (synaptosomes) were prepared by centrifugation on Percoll gradients
When used for neurotransmitter release experiments, synaptosomes were resuspended in physiological medium with the following composition: 140 mM NaCl, 3 mM KCl, 1.2 mM MgSO4, 1.2 mM CaCl2, 1.2 mM NaH2PO4, 5 mM NaHCO3, 10 mM glucose, 10 mM HEPES, pH 7.2–7.4. For western blotting experiments, synaptosomes were resuspended in lysis buffer: 120 mM NaCl, 20 mM HEPES pH 7.4, 0.1 mM EGTA, 0.1 mM DTT, containing 20 mM NaF, 5 mM Na2PO4, 1 mM Na2VO4, and 2 mg/ml of protease inhibitor cocktail (Sigma-Aldrich, Milan, Italy). Further fractionation of freshly purified synaptosomes into synaptic membranes was carried out by differential centrifugation as previously reported
Endogenous glutamate and GABA release experiments
Endogenous neurotransmitter release was measured as previously reported
Fractions collected were analyzed for endogenous glutamate and GABA content. Endogenous glutamate and GABA were measured by high performance liquid chromatography analysis. Amino acid release was expressed as nmol/mg of protein. The stimulus-evoked overflow was estimated by subtracting transmitter content of the two 3-min samples (basal outflow) from release evoked in the 6-min sample collected during and after the depolarization pulse (stimulus-evoked release). Drug treatment effects were evaluated by comparing the stimulus-evoked overflow in drug-treated animals vs. that calculated in vehicle treated rats. Appropriate controls were always run in parallel.
Measurement of SNARE complex in isolated presynaptic membranes
For detection of SDS-resistant SNARE complexes, Western blotting was performed on samples of electrophoresed presynaptic membranes (non-boiled before gel loading)
Statistical analysis
Statistical analysis was carried out by one-way analysis of variance (ANOVA) followed by Newman-Keuls post-hoc test. Significance was assumed at p < 0.05. Statistical analysis of the data was carried out using GraphPad Prism4 (GraphPad Software Inc., USA). Data are expressed as mean ± SEM.
ANOVA: Analysis of variance; GABA: γ-Amminobutyric acid; MT1: MT2, Melatonergic receptor1 and 2; RRP: Readily releasable pool; SNAP-25: Soluble NSF Attachment Protein-25; SNARE: Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor.
Competing interests
MP received research support and/or has been consultant for: Abiogen, GlaxoSmithKline, Merck Sharp & Dohme, Abbott, Servier, Fidia. GR has scientific collaborations with and is a member of the scientific board for Eli Lilly, Innova Pharma, and Servier. EM and CG are full-time employees of Institut de Recherches Internationales Servier (I.R.I.S.). MM, DT, LM, GT, AM, TB and GB: nothing to disclose.
Authors' contributions
MP, GB and GR designed the study. EM and CG participated on study design and paper discussion. DT, LM and MP wrote the ms. MM, AM, GT, TB performed the experimental work. DT, MM and LM performed the statistical analysis. All authors read and approved the final manuscript.
This study was supported by a research grant from Servier. GT was funded by the International PhD Program in Neuropharmacology, University of Catania.
Linking molecules to mood: new insight into the biology of depression
Neural circuits underlying the pathophysiology of mood disorders
Towards a glutamate hypothesis of depression: an emerging frontier of neuropsychopharmacology for mood disorders
Mapping the matrix: the ways of neocortex
The stressed synapse: the impact of stress and glucocorticoids on glutamate transmission
Signaling pathways underlying the pathophysiology and treatment of depression: novel mechanisms for rapid-acting agents
Targeting the glutamatergic system to treat major depressive disorder: rationale and progress to date
Overview of glutamatergic neurotransmission in the nervous system
Roles of glutamate signaling in preclinical and/or mechanistic models of depression
The action of antidepressants on the glutamate system: regulation of glutamate release and glutamate receptors
Chronic antidepressants reduce depolarization-evoked glutamate release and protein interactions favoring formation of SNARE complex in hippocampus
Serine/threonine kinases as molecular targets of antidepressants: Implications for pharmacological treatment and pathophysiology of affective disorders
How can drug discovery for psychiatric disorders be improved?
Acute stress increases depolarization-evoked glutamate release in the rat prefrontal/frontal cortex: the dampening action of antidepressants
New selective ligands of human cloned melatonin MT1 and MT2 receptors
The novel melatonin agonist agomelatine (S20098) is an antagonist at 5-hydroxytryptamine2C receptors, blockade of which enhances the activity of frontocortical dopaminergic and adrenergic pathways
Agomelatine, the first melatonergic antidepressant: discovery, characterization and development
Mode of action of agomelatine: synergy between melatonergic and 5-HT2C receptors
Blockade of stress-induced increase of glutamate release in the rat prefrontal/frontal cortex by agomelatine involves synergy between melatonergic and 5-HT2C receptor-dependent pathways
SNARE complex formation is triggered by Ca2+ and drives membrane fusion
Molecular machines governing exocytosis of synaptic vesicles
Calcium control of neurotransmitter release
Acute stress-mediated increases in extracellular glutamate levels in the rat amygdala: differential effects of antidepressant treatment
The antidepressant agomelatine inhibits stress-mediated changes in amino acid efflux in the rat hippocampus and amygdala
Morphological correlates of functionally defined synaptic vesicle populations
The synaptic vesicle cycle
A rapid method for isolation of synaptosomes on Percoll gradients
Glia re-sealed particles freshly prepared from adult rat brain are competent for exocytotic release of glutamate
Chronic antidepressants induce redistribution and differential activation of aCaM kinase II between presynaptic compartments
Coexistence and function of different neurotransmitter transporters in the plasma membrane of CNS neurons

subset: commoncrawl


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Mont-Tremblant, Québec
- 4 卧室
- 2 浴室
- 1 半浴室
Superb '' Post and Beam '' home within an exclusive community on pristine lake Desmarais. Warmth, comfort and elegance best describe its architecture and design. Open concept, sunken living room with wood burning fireplace, cathedral ceilings, gourmet kitchen with granit counters, and so much more. Large decks facing the lake, natural landscaping.
- Raymond Dalbec
- 乡村田园生活
- 高尔夫
- 湖
- 湖畔
- 滨水区
- 滑雪
- 滑雪物业
- 10,086.20 m2 / 108,570.51 ft2 - 地面积
- 建成年份 2007
- $8,733 税项 市政 2015
- $1,584 税项 学校 2014
Features of this superb property: Quality, architecture, location, decor, interior and exterior design, spectacular views, private setting, exclusive community, garden landscaped in harmony with nature.
51.76 linear meters ( 170 feet ) on lac Desmarais, one of the most beautiful lakes in the area where peace and serenity rule. Natural habitat to indigenous to Touladi ( grey ) trout, and stocked with speckled trout. 2 Loon families return every Summer. Surrounded by Nature, yet 10 minutes to the activity. PARADISE.
Less than 15 minutes to the Mont-Tremblant Resort and Ski Area.
'' Normerica '' Post & Beam construction. Cathedral ceilings in living room and master bedroom. Vaulted ceiling over gourmet kitchen, granite counters. Marble counters in bathrooms, and radiant heated ceramic floors. All open space rooms and bedrooms have great views of the lake. Refrigerated wine cellar. All interior doors are antiques from different origins, and are very finely restored. Integrated loudspeakers in living areas and outdoors. Cozy family room on garden level, with gas fireplace. Steam humidifier system. Forced air electric heating. Convectair type additional heating on garden level.
Outdoors: Large 60 foot cedar deck facing the lake, including a nicely coverd area, gazebo-like.
Irrigation system in the landscaped portion of the property.


text:
16 July 2008,
London, South Kensington
Price realised GBP 2,500
Simon Palmer (b. 1956)
Following the Visiting Preacher
signed 'Simon Palmer' (lower right) and inscribed 'Following the Visiting Preacher' (lower left)
pencil and watercolour
20¼ x 15 in. (51.4 x 38.1 cm.)
New York +1 212 636 2000
London +44 (0)20 7839 9060
Hong Kong +852 2760 1766
Shanghai +86 21 6355 1766
Special Notice
No VAT will be charged on the hammer price, but VAT at 15% will be added to the buyer's premium which is invoiced on a VAT inclusive basis.Artist's Resale Right ("Droit de Suite"). Artist's Resale Right Regulations 2006 apply to this lot, the buyer agrees to pay us an amount equal to the resale royalty provided for in those Regulations, and we undertake to the buyer to pay such amount to the artist's collection agent.
with Forest Gallery Fine Art, Guildford.
Overall sales hit £2.35 billion ($3 billion) in the first half of the year, with auction sales up by 29 per cent on the e..
Interior designer and heritage colour expert Edward Bulmer on the modello by Jean-Baptiste van Loo he acquired 27 yea..


text:
Hello it's me again (damn these ideas just keep coming to me) anyway, a few days ago i was in a chat and i was talking about (if you have not read the title) Songs from Sonic the Hedgehog fits Fairy Tail? some of you who were there on the chat (such as Lisa and Red) should remember, i was just putting up links to the video and the names of the characters that fits and that is where i got the idea to do this. and there are a few songs i think fits some of the characters.
Natsu: His World, from Sonic 06 or It Doesn't Matter, from Sonic Adventure 2 Battle.
Jellal: Live and Learn, from Sonic Adventure 2 Battle, or Who I Am, from Shadow the Hedgehog.
Juvia: Follow Me, from Sonic Heroes, or My Sweet Passion, from Sonic Adventure DX.
Laxus or Elfman: What I'm Made Of, from Sonic Heroes.
Zeref: (at first i was thinking Jellal but i was going there the lyrics again and it hit me) Throw It All Away, from Sonic Adventure 2 Battle, or I Am... All of Me, from Shadow the Hedgehog.
That is all i can think of right now. if your a Sonic fan like me then check out some of the songs and try to think of witch one fits who? and i want to hear thoughts in the comments down below, i will be happy if you do. and now i'm signing out.
Edit: can someone make YouTube vids of these songs with the characters i would love you forever.


text:
by Lara
Whenever someone asks me to tell about a time in my life when I was really happy, I immediately think about a certain picture. There is me. There is daddy. There is mommy. I am about 5, they're holding my hands, and I'm smiling.
I have no memories of that day, but I just KNOW I was happy.
And then, life begins.
My grandparents all died before I was even 5.
When I was 6, it was my dad's turn - cancer due to asbestos.
For 19 years of my life, it was just me and mum alone.
She had to raise me by herself, and had never really overcome my father's death. Nor had I, truth to be told.
It was a sort of taboo in my house, and we never really talked much about it. I knew she resented him, and I knew she always thought I was fine, because I never cried about it.
But I wasn't fine, and she wasn't fine, but we lived.
My mother wasn't one for social gatherings, nor parties, nor celebrations.
I never walked inside a restaurant with her, if not for very important events. Never went for a walk that hadn't a purpose, like grocery shopping. Almost never went on holiday.
She was my shell.
She almost suffocated me at times, and she never let me do things most of my peers did. Never a sleepover, not many parties. No going on holiday with my friends when I was a teenager.
But I never really complained, because I thought that my life was good, and perfect and that I was lucky.
And then, I was 20 or so, all the emotions I had kept bottled for 14 years nearly exploded.
I was angered, depressed, I was sad.
I missed my dad for the first time in 14 years.
And mum started to believe she had done something wrong.
In the heat of the moment I maybe blamed her for things that weren't entirely my fault, but she did the same.
My perfection started to become imperfect.
Then 5 years on and off - I went from a great student, to one who fell three years behind in university. I just wanted to do nothing, because I had no motivation to go on. I don't have many friends. Never had many to begin with.
I have no job, no university degree.
And, finally, two weeks or so ago.
August 6th, 2012.
My mum had had a few health problems, recently.
A problem with her knee, which was nothing serious, and only needed rest - then a reaction to a medicine, but again nothing much. And then a bad, bad cough, with throwing up - something we were used to, she had delicate airways.
It went on for about a week.
The doctor was supposed to come on August 6th, early in the morning, to see why she didn't want to eat, or why her cough was so bad.
During the same time, I was told again my dog needed to ungergo surgery AGAIN for cancer and so (and I will always be guilty for this), I was a little more worried about her, than about mum - mum was strong, and then doctors had seen her TWICE and said it was okay.
I checked on her every hour or so, even at night, to make sure she was alive.
I did it every night, minus that night.
She had coughed a lot, but then the cough had subsided, thanks to a few cough suppressant drops, just like the other days.
I was talking a friend about my dog, about how I worried too much - about my stupid problems.
And, in the meanwhile, she was dying.
She died in her sleep due to natural causes.
I found her at 5.30 AM - I just wanted to wake her up, and ask her about the doctor, you know, what time she would come home to visit her, and what I needed to do.
But she was already cold, and rigid, and even if I tried to revive her, it was way too late.
I kissed her hands, and cried, and screamed in the middle of the night.
My friend who was there held me back - I tried to commit suicide like 4 times in the 45 minutes we had to wait for the ambulance.
I sliced my arm open, I hopped on the railing of my balcony and tried to jump down, I started bumping my head hard against the wall.
My family reached me the following day.
They said they wanted to help me clean the house, because I had been so busy tending to mum, lately, that everything was a mess - and we had a sick dog in too, so the problems tenfolded.
The result?
My keys disappeared, a few money I had in a box disappeared, and I had to hurriedly change the lock to make sure my relatives couldn't break in.
Up to now, I still don't know how many things are really missing.
So, my family, instead of helping me, robbed me.
They blamed me for my mum's death, they didn't even give money for her funeral - I had to pay it all myself, with about 1/3 of mum's savings.
They said I had kidnapped my mum, because she used to tell them that she didn't want to go to their gatherings because of me ("She's young, she would have no one to talk to."), when she just didn't want to be around those people. I was okay with taking the blame, if it was for mum's wellbeing.
They said I didn't care, because I asked them to organize the funeral - but I was too shaken, I was on anti-anxiety medication, and I am an atheist, while mum was a fervent catholic, like them. They would surely know better about a standard catholic ritual. And then, I paid every single cent of it. And yet, according to them, I didn't care.
They said that I was not normal, beacuse I wasn't crying in front of them. They said I was "selfish and concerned about myself" because I was brought by my friends to a psychologist, due to my suicide attemps and suicidal plans. He even said I shouldn't attend the funeral, due to my conditions, but I was forced to attend, to avoid a major fight with my relatives.
Since then, I have cried maybe 10 times, for just a couple of minutes each time.
I have accepted her death, I think - I have found her, I have tried to relieve her, I was there to see her body...
And yet, I don't cry.
I feel sad, hollow. I don't want to live this life.
I have no job, little money to go on (maybe a few months?), but I'm obviously not in the right state of mind. I can't work, nor study right now. I always loved writing, and I'm trying to work on my comic novel, the one I cared so much about, before...but right now...
I can't.
A friend of mine has been living with me and mum, for the last few years (he was closer to his job, this way), and always contributed to the expenses. He can provide for me, for the time being - my mum was a bit like his mum too, for the last 5 years.
I see nothing worth living for, right now.
My dad abandoned me. My mum abandoned me.
I keep dreaming that they tell me it's all a huge mistake, and that mum is alive and well. I hug her in my dreams, and I say the same exact things I used to tell her in real life "Please, take care of your health, mum, please, I don't want to lose you. I can do only this much, but the rest is up to you."...
And then, I wake up, and reality hits me.
I have begged her multiple times.
To take some days off from work, to take care about herself... and she always blamed me.
"If only you had your university degree, I could slow down.", or "If only you helped me more..."
And so I tried my best.
My university studies were going good again, after 3 years of almost nothing. I cleaned the housed and cooked all by myself, even if she never ate any of my food.
She didn't even want to try it.
And now I feel guilty.
If only I had checked more often on her that night, too.
If only I hadn't felt depressed for 5 years, and had completed university.
If only, if only...
My life has lost all meaning now.
And what I'm scared about, is that it will get worse day by day. I have resisted many, many losses already in my life. More than you can imagine. And this is just the final blow.
I don't want to miss her anymore than I'm already doing.
I hardly ever eat, I can't sleep more than 4 hours, and am I just... the shadow of the positive, energetic girl that I was.
I just want this to end.
Will luck ever turn my way?
I am only 25.
And I have already endured what most people will endure in a whole lifetime - my grandparents died, my father, my mother, who was only 64. My family robbing me. My best friend disappearing.
I can't just take any more pain.
I just can't.
Thanks for reading, and good luck to everyone.
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This page consists of a note written by Mandela in which he discusses his involvement with the armed wing of the ANC, the Umkhonto we Sizwe (Spear of the Nation), also knowned as 'MK'.
Nelson Mandela Centre of Memory
Title: Notebook page - Umkhonto we Sizwe
Description: This page consists of a note written by Mandela in which he discusses his involvement with the armed wing of the ANC, the Umkhonto we Sizwe (Spear of the Nation), also knowned as 'MK'.
Date: 1991/1991
Original Language: English
Transcript: I was one of those who formed MK, and my instructions from the NEC of the ANC was to attend the Pafmecsa Conference in Addis Ababa in February 1962; to visit the independent African States, and to ask them to give military training to our people, and to raise funds for the struggle.
I had lived underground for almost 10 mnths, and it was exciting indeed to look forward to free and unfettered movement in daytime, without fear of arrest.
I looked forward to visiting new countries and meeting famous freedom fighters, who were dominating the liberation movement in various parts of the continent.
Above all, I looked forward to meeting Comrade Tambo and his capable team which was mobilising international support for our struggle. They had done impressive spade work and helped to put our struggle on the map. Emergence of MK had boosted their efforts. but socio-economic problems were frightening.
Type: Diary/Calendar
Reference code: ZA COM NMPP-2009/8-1-9-14
Extent and Medium: 1 page
Repository: Nelson Mandela Centre of Memory
Immediate source of acquisition or transfer: Deposited by Nelson Mandela
Conditions governing access: Access by permission of Nelson Mandela Centre of Memory
Conditions governing reproduction: Copyright held by Nelson Mandela Centre of Memory
Finding aids: Finding aid available
Creator: Mandela, Nelson
Subjects: Umkhonto we Sizwe (MK)
Collection: Notebooks and Notes, 1990 - 2001
Display Date: 1991/1991


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ASSOCIATED WITH mountain
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Inside Out Memory
hi dear! I guess that you like logic, puzzle and memory game. Now, in front of you , there is an amazing memory game, named "inside out memory". You can play it right now. The game is not easy at all, when you start it you will see 15 same images. When you click on them you will see characters from movie inside out. You must remember what characters are behind each image, and click a same character, than this two image will disappeared. You will earn points on every correct turn and loose the in every wrong turn. So, be careful about your points and remember the characters. Good luck!!!
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Volusia Buddhist Fellowship has been invited to get more involved at White Sands Buddhist Center in Mims, giving us an opportunity to add more services and reach a larger community. We have scheduled monthly Sunday morning services and meditation on the first Sunday of each month. The morning's events will begin at 9:00 a.m. with an hour of meditation, including guided meditation, walking meditation and sitting meditation.
Then, after a half-hour break, we will begin a service at 10:30 that will have a very special format with chanting, readings and dharma talks from Rev. Morris and other ministers and lay members. You are welcome to attend just one or both sessions.
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I need to create a batch file to terminate a group of processes relating to my company's security software. Several machines were cloned and now have the same GUID, so the security software will only recognize the original server side. After the processes are killed the batch file deletes the registry entries containing the GUID and restarts the security software, generating new GUIDs in the process. I can kill all of the process that I need to using Windows Task Manager, but when I try to use taskkill from a cmd with admin privileges I get:
C:\scripts>taskkill /IM coreServiceShell.exe /f
ERROR: The process "coreServiceShell.exe" with PID 1612 could not be terminated.
Reason: Access is denied.
Any help would be greatly appreciated :D
15 Replies
Jul 5, 2012 at 3:04 UTC
This is how I have been killing which looks like mostly a switch reorder of yours (not sure if it matters):
taskkill /f /im service1.exe /im service2.exe
What account are these services running as?
Jul 5, 2012 at 3:24 UTC
You could try entering the computers' names to the batch file to run on multiple machines.
taskkill /s ComputerName /f /im ServiceName.exe
Jul 5, 2012 at 3:38 UTC
are you running Cmd prompt as an admin? it matters is windows 7. right click cmd and click Run as admin, click ok on the prompt, then admin cmd will start. try then
*edit, even if you have admin privileges selected under properties, window's 7 is picky with it
Jul 5, 2012 at 7:52 UTC
Matt and Nicole, I will definitely try those suggestions tomorrow when I get to work!
Mr. Cyberspace, thank you for the advice, I'm running XP Professional, but we do have several computers in the company running windows 7. I've tried running cmd both using my domain account (which has admin privileges) and using the local administrator account. I've also tried using psexec -i -s cmd (which I believe should run cmd using the system account right?)
I'll update my progress after giving all of your suggestions a try!
Jul 6, 2012 at 5:15 UTC
Never used psexec but you could make sure the whole string is running as admin from cmd, looks like this:
runas /U:email@example.com "psexec -i -s cmd"
runas /U:firstname.lastname@example.com cmd
To create a new cmd window running as the user you specificed :)
Jul 6, 2012 at 6:48 UTC
Hey all, I've tried the above suggestions and still no luck :(
Here are the commands that I'm using to kill the processes
taskkill /f /IM AMSP_LogServer.exe
taskkill /f /IM coreFrameworkHost.exe
taskkill /f /IM coreServiceShell.exe
taskkill /f /IM uiWatchDog.exe
taskkill /f /IM TmListen.exe
taskkill /f /IM uiSeAgnt.exe
taskkill /f /IM uiWinMgr.exe
The account I'm running it on right now has local and network admin privileges. The worst part is that I can still kill them form Task Manager >.<
I've also tried using tskill processname
Most of the processes are running under SYSTEM, but again, I can terminate them using task manager...just not practical to vnc into each computer that needs to be fixed...
Jul 6, 2012 at 8:23 UTC
I've had luck using psexec to do stuff like this.. sometimes I use 2 bat files...
file 1... psexec -u "Domain\Username" -p "PASSWORD" @Path\To\list of comptuers.txt \\Path\To\withcommands.Bat
File 2... taskkill /f /IM AMSP_LogServer.exe
taskkill /f /IM coreFrameworkHost.exe
taskkill /f /IM coreServiceShell.exe
taskkill /f /IM uiWatchDog.exe
taskkill /f /IM TmListen.exe
taskkill /f /IM uiSeAgnt.exe
taskkill /f /IM uiWinMgr.exe
Jul 6, 2012 at 9:12 UTC
Running as the System account is probably the problem. I think there is an additional trick and this might be it. Running through the GUI assumes some things, but as the CLI throws a few flags that aren't always shown.
taskkill /f /fi "USERNAME eq NT AUTHORITY\SYSTEM" /im notepad.exe
Jul 6, 2012 at 9:18 UTC
Thanks Mark, but still nothing. Is there any way to access task manager from cmd, without a gui? Just use the end process function on a specified process?
Jul 6, 2012 at 9:37 UTC
Have you tried restarting the services prior to running taskkill?
If that doesn't work you can you try to create another account with admin privileges and see if taskkill works with that account? It could be something weird with the account.
Jul 6, 2012 at 9:44 UTC
The services related to the security software are also protected.
When I try:
C:\Documents and Settings\jgree>net stop "Trend Micro Solution Platform"
The requested pause or stop is not valid for this service.
More help is available by typing NET HELPMSG 2191.
C:\Documents and Settings\jgree>net helpmsg 2191
The requested pause or stop is not valid for this service.
This command is invalid for this service, or the service cannot accept the comma
nd right now.
If the service normally accepts this command, try typing it
again later.
To display a list of valid commands, type:
I've tried downloading several other process killing utilities (just to test) and none of them can kill the processes either. Only Task Manager so far :(
Jul 6, 2012 at 10:26 UTC
you say task manager works.. on the same machine, can you use the command line.. or are you trying that only on remote machines..???
one thing I do on occasion is to open the command line as the administrator and run commands that way..
Jul 6, 2012 at 10:54 UTC
Sorry, I'll clarify. I need to terminate several processes. I will need to do so silently and remotely, but for now I'm working on my local machine. I can use the task manager on my computer to terminate these processes, but not using taskkill, also on my local machine. The reason I need it done in command line is because we really want to limit our vnc use because whoever normally would be using the computer isn't very productive when we take control...
Jul 6, 2012 at 11:03 UTC
i've had similar situations......
I used PSList to show me the task names
then used either taskkil or PSKill to end the process.
one more thought.. from the command line run... "runas /user:domain\administrator" cmd
it will ask for the password.. and then you can run commands as the domain admin.. if that can't kill it from command line.. I don't know where to go from there.
Jul 6, 2012 at 11:11 UTC
Thanks Robert, but still no go.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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SATURDAY ARRIVALS ONLY - The Hotel Alaska is set back from the main road in the centre of Livigno and is surrounded by shops, bars and restaurants. The slopes are 300 metres away and it is just 25 metres to the nearest ski bus stop. The hotel offers simply furnished two and three bedded rooms as well as a waiter service restaurant and a lounge bar serving snacks. Guests can also park their car at the hotel, store ski equipment and make use of the wireless internet access on offer.
The duty-free resort of Livigno nestles between the North and Southern Alps, close to the Swiss border and is the perfect choice for those seeking great skiing and lively good value apres ski. There are loads of shops, bars, restaurants and nightclubs to entertain you after a long day on the slopes. The ski area in Livigno is best suited for beginners and intermediates as there are plenty of long, wide-open slopes and excellent ski schools.
Distance from Airport:
140 miles - Milan
All have * bathroom/shower * WC * safe * TV * telephone * hairdryer * (certain amenities may be payable locally)
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180 minutes
Eating and Drinking:
The hotel has a waiter service restaurant and a lounge bar that serves light snacks.
Swimming pools:
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Right choice:
This hotel will appeal to couples, families and groups looking for stylish accommodation in a great location for a ski holiday in the lively resort of Livigno.
Suitable for:
Couples, Families and Groups Beginners and Intermediates


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Japan has lots of cat cafes (like this Studio Ghibli cat cafe), and now we finally have one in the US. Cat Town Cafe is now open in Oakland, California. Owners Ann Dunn of Cat Town Adoptions and Adam Myatt want it to be a place where people can relax with their cats and maybe even find one to adopt. According to the website:
The Cat Town Cafe will feature an area called the Cat Zone, where our cats will be free roaming and available for adoption. Visitors can spend up to an hour in the Cat Zone, and free walk-ins are welcome as space allows. For a $10 donation, you can make a Cat Zone Reservation ahead of time, which ensures your visiting time is available, and supports a great cause.
If you're in the Oakland, California area, make a reservation to hang out with some cats and maybe even take one home.
See more pictures after the break.
(Fast Company via Laughing Squid)


text:
The Leviathan - Huge PVC Sculpture
Just when I thought I'd scoured the internet for every cool existing PVC project, this thing came up.
There are also pictures from the inside of this thing.
- You must have a strong stomach to image search (insert adjectives here) PVC with safesearch off. There is some stuff that I wish I had never seen.


text:
Hochstetler Collection
Call one of our Amish furniture specialists at 1-866-272-6773 for more information on our Mission Sofa Table! This Amish Sofa Table is available in Red Oak Wood, Cherry Wood, Hard Maple Wood, Brown Maple Wood, Quarter Sawn White Oak Wood, Ash Wood or Rustic Cherry Wood. After an order is placed, we are happy to send out finish samples on your wood selection free of charge. This will allow you to match your hardwood sofa table up to the other pieces in your home.
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Lipton served on an NYSE committee examining the exchange's compensation and disclosure policies while also counseling Grasso, according to the lawsuit that New York Attorney General Eliot Spitzer filed against Grasso Monday. The lawsuit, which does not accuse Lipton of wrongdoing, seeks to rescind much of Grasso's $187.5 million package for being excessive and illegal.
Grasso resigned from the NYSE last September after his pay package became public. He says his compensation was justified.
Last September, after Grasso disclosed that he would be receiving a lump-sum payment of $139.5 million, Securities and Exchange Commission Chairman William Donaldson fired off a letter to the Big Board demanding an explanation for the large sum.
The Spitzer complaint says that Lipton's firm, Wachtell Lipton Rosen & Katz, "played a significant role in drafting the response to the SEC." The complaint also says that Wachtell lawyers "did not suggest" revising an earlier explanation of Grasso's compensation that was inaccurate.
Spitzer's complaint quotes an e-mail that Lipton sent to an unnamed friend, acknowledging that he gave Grasso advice on how to handle the matter of an additional $48 million in compensation he was owed.
Lipton's firm said he was out of the USA and unavailable to comment. Lawrence Pedowitz, another partner at the firm, did not return calls for comment.
Critics of the NYSE, as well as threelegal experts, did not see ethical violations in Lipton's actions but questioned the appearance of a conflict of interest.
"There was a culture of coziness" at the NYSE, says Nell Minow, editor of The Corporate Library, a shareholder watchdog group. "This is not the worst example, but yet another instance where people were not thinking of how this was going to look. I could see how Marty Lipton, who's a good guy, would provide advice. But he should have said, 'Get a lawyer.' Do I think Marty Lipton violated the canon of ethics? No. Did he make an unusual mistake for a smart lawyer? Yes."
Cynthia Williams, an associate professor at the University of Illinois College of Law, says Lipton's advice to Grasso could have been consistent with his counsel to the board. But, "lawyers are well advised not only to avoid improprieties, but the appearance of improprieties."
Meanwhile, Grasso plans to sue the NYSE for the $48 million that he believes he is still owed and to seek compensation for damages to his reputation, says his spokesman Eric Starkman. Grasso vows to give the proceeds to charity.
Contributing: Thor Valdmanis


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Data from: Small beetle, large-scale drivers: how regional and landscape factors affect outbreaks of the European spruce bark beetle
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Definition Edit
A reference architecture in the field of software architecture or enterprise architecture provides a template solution for an architecture for a particular domain. It also provides a common vocabulary with which to discuss implementations, often with the aim to stress commonality.
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Please confirm that you want to add SAP Crystal Reports with ASP.Net (Step by step) to your Wishlist.
Course Description:
Learn the easiest and basic method to work with SAP Crystal Reports 2013 in Microsoft Visual Studio 2012 connected to MS SQL Server 2008 Database.
Let's make the first move to design & display one simple Crystal Report for the professional presentation of data in a web browser, in a manner that could be used for decision making by the management.
Course Specialty:
This course will help software developers to get rid-off writing complex & lengthy code for displaying Crystal Report in a web browser. This course demonstrates a "Developers-Friendly" method of:-
Software/s and their versions used in this course:
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I am a Microsoft Certified Professional since 2002 and holding Degrees in Computer Science, Business Administration and General Management. I entered into the field of IT in 1994 with a diploma in computer science from Pakistan and then went to England where I continued my studies and acquired diploma in Computer Networking and Information Management in 1998. As a software developer, my career started when i became MCP in 2002 and developed a complete ERP System software by the end of 2004. I worked a lot in Crystal Reports in the ERP system with integration in both Desktop & Web Applications. I designed several complex Crystal Reports during the journey of various versions of Crystal Report from versions 8.0 to 2013. My expertise are
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