text:
'Blue Bloods' feasts on family bonds
NEW YORK Sunday dinner and shared sacrifice add to the appeal of CBS' hit police procedural.
It's dinner time for the Reagan clan, and the extended family of CBS' Blue Bloods is crammed around the table, sharing the news that their parish priest has been sent off to Bolivia after allegations of sexual advances, in an episode airing tonight (10 ET/PT).
It's the only time the show's entire main cast, led by Tom Selleck, Donnie Wahlberg, Will Estes and Bridget Moynahan, comes together in its Brooklyn studio.
"Luckily we don't have to eat anything crazy like cheesecake or Chinese food," says Moynahan (who plays daughter Erin, an assistant DA) of Bloods' signature scene, which caps every episode and gives writers a chance to show the family debating the issues raised by the cases.
"I think the audience responds to these things more than any part of the show, really. It's a place where you get everybody's point of view."
It could be the tight-knit family, or Selleck's star appeal, or the unusual blending of a family drama with a sturdy police procedural, but Bloods is this season's most-watched new series. It chugs away on Friday nights, with an average of 12.4 million viewers, and is considered a lock for a second season.
The show was conceived by veteran producer Leonard Goldberg (Charlie's Angels); married Sopranos writers Robin Green and Mitchell Burgess created the show and oversee its writing staff.
Both were attracted to switching from mobster anti-heroes to a family of NYPD heroes, and took inspiration from the Kennedys and The Godfather. "There were elements of those two iconic American families that were part of our thinking," Green says. "Writing's stealing, right?"
Bloods connects its cases to the multi-generational family, led by police commissioner Frank (Selleck), a stoic police commissioner who's sometimes at odds with a mayor who appointed him when his last pick didn't work out.
"He was a way of getting the mayor off the hook for choosing a corrupt man," Selleck says of his character. "His biggest flaw is he cares too much; he has a hyperactive sense of responsibility."
His dad Henry (Len Cariou), a retired commissioner, is a grandfatherly fixture at those dinners, even though Cariou, 71, is actually just five years older than Selleck. Erin (Moynahan), a divorced single mom, is the by-the-book legal compass. But Danny, a hotheaded cop, is an Iraq War vet who "may bend the rules because he knows he's going to get away with it more," Wahlberg says.
"He's going to do what's right to get the job done," even if that means agreeing to testify against a drug lord, which led to the kidnapping of his wife, Linda (Amy Carlson), in last week's episode. (Linda's troubles aren't over: Tonight, her fashion-model niece is poisoned during a runway show.)
And there's Jamie, a Harvard law grad who couldn't resist the family business and became a beat cop after another brother, Joe, was killed in the line of duty. "He's a smart cop, he's got empathy for people," Estes says. But despite his Ivy League pedigree, "there's no replacement for experience. His learning curve is the school of hard knocks just the same."
Now Jamie is part of Blue Templar, a covert squad investigating Joe's death. That's the focus of this season's final three episodes, starting April 29. "The death of the son has impacted this family hugely," Green says, "so the last episode emotionally is about putting Joe to rest."
The pilot was filmed mostly in Toronto, but Selleck and Goldberg insisted the show be shot in New York, where Bloods inherited the Big Apple cop beat from the original Law & Order. Cast and crew travel frequently to tap the city's culture, filming in Chinatown, the Russian neighborhood of Brighton Beach and elsewhere.
But those dinner scenes, which can take hours to film, bring the Reagans home. "I grew up doing big dinners, and to me, looking across at Bridget is just like looking across at my sister," Wahlberg says. "I love my sister more than anyone on the face of the earth, but we fight like cats and dogs."
Posted | Updated


text:
The UC Berkeley Green Event certification identifies and recognizes campus events whose organizers have taken extra steps to lower their environmental footprint. The program identifies what qualifies as a green event, including required and optional criteria. We can assist you in greening your event!
Here are some ways to make your event green:
If you are interested in making your event green certified, visit the Office of Sustainability's Green Event certification page to learn more.
Phone: (510) 642-1141 E-mail: firstname.lastname@example.com Office: 2495 Bancroft Way, MLK Jr. Building, 2nd floor, Berkeley, CA 94720

subset: commoncrawl


text:
I am an experienced and highly-qualified English teacher (C2 level, Cambridge English Proficiency). I have lived in US and graduated from University in US. I have a 10 years of experience of working in telco industry within a British company, I understand the corporate environment and thus can teach business english. I have worked as both interpreter and translator prior to my telco career.
Let me know if you are interested in lessons from beginner to business level. I mainly focus on conversation, but I can guide you through grammar as well, or prepare for job interviews (I speak a bit of Czech, sometimes it helps ;) C ya, Milos
Doporucuji. Hodiny anglictiny s Milosem mi pomohly ziskat sebevedomi v anglicke konverzaci a k tomu utekly jak voda! :)


text:
Right-Click-Link Firefox:1.0–3.0.* add-on
Use this security logo:
Freeware is computer software that is available for use at no cost or for an optional fee.
Choose The Right Jeans
Advanced Hiking Tips : Pack The Right Equipment For Hiking
Youth Sports : Eat Right For Youth Soccer
Photograph A Model: Photo Studio Tips : Choose Right Umbrella For Photo Shoot
Play "Right Here Waiting For You" Pt 1
Updated for 3.0.* support.


text:
My Mom And My Girlfriend 4Favorites [+]
Pay-Per-Minute Viewing
You can now enjoy this movie on your iPhone®, iPad®, or compatible Smart TV & Android devices.
Purchase Options
Pay-Per-MinutePurchase Minutes
Available MP4 sizes:SD 480p (1.73 GB)
DRM-free downloads offer content that is not limited by standard Digital Rights Management (DRM) access technologies. These technologies allow hardware manufacturers, publishers, copyright holders and individuals to limit the usage of digital content.
When you purchase DRM-free downloadable content, that content is available for you to download on any computer and save to a disk whenever you like. You can also download to multiple computers. There is no timeframe associated with your purchase and the content will not expire.
Running Time: 110 minutes
Released: 03/2007
Studio Name: Filmco
Series: My Mom And My Girlfriend
Description: May/December thinks pink as hot mamma gets it on with daughter's younger girlfriends. Real lesbian lovin' between an older woman and a younger girl is smoking hot!
5 Scenes from My Mom And My Girlfriend 4
Watch with a Subscription to the SuperPass Channel, with Pay-Per-Minute or Streaming Rental
My Mom And My Girlfriend 4: Scene 1 Free Preview
Starring: Sindy Cummings Austin O'Riley ... More Details
Stars: Sindy Cummings, Austin O'Riley
Sex Acts: Breast Play, Vaginal Sex, Oral Sex, Strap-On, Masturbation
My Mom And My Girlfriend 4: Scene 2 Free Preview
Starring: Marquetta Jewel Kayla Kupcakes ... More Details
Stars: Marquetta Jewel, Kayla Kupcakes
Sex Acts: Breast Play, Facesitting, Grinding, Masturbation, Vaginal Sex, Oral Sex
Positions: Kneeling, Laying, Crouching/Squatting, Standing
My Mom And My Girlfriend 4: Scene 3 Free Preview
Starring: Samantha Foxx Penny Porsche ... More Details
Stars: Samantha Foxx, Penny Porsche
Sex Acts: Breast Play, Masturbation, Vaginal Sex, Oral Sex
My Mom And My Girlfriend 4: Scene 4 Free Preview
Starring: Anastasia Sands ... More Details
Stars: Anastasia Sands
Sex Acts: Breast Play, Masturbation, Oral Sex, Vaginal Sex
Settings: Kitchen
My Mom And My Girlfriend 4: Scene 5 Free Preview
Starring: Nicole Moore Brooke Banner ... More Details
Stars: Nicole Moore, Brooke Banner
Sex Acts: Toy Play - Vaginal, Breast Play, Grinding, Masturbation, Pissing, Vaginal Sex
Recommended Movies
AEBN Cams


text:
In a story that dominated the headlines, James B. Stewart takes readers inside the epic battle for control of the world's leading entertainment company, Disney.
Die Inhaltsangabe kann sich auf eine andere Ausgabe dieses Titels beziehen.
James Stewart has done it again. The author of the mega-bestselling Den of Thieves, about the 1980s insider-trading scandals on Wall Street, and Bloodsport, the 1990s tale of the Clintons' Whitewater affair, now gives us another epic story, this one culminating in late 2004. With DisneyWar, Stewart turns his investigative and storytelling lens on Michael Eisner and the corporate intrigue which has overtaken the Walt Disney Company in the last decade. He explains how this once-proud institution, long one of America's most admired and well-known businesses, has stumbled in recent years amid a disastrous swirl of egos, personalities, and bad business decisions.
Like one of the roller coasters at DisneyLand, Stewart's epic book takes readers through a wild up-and-down ride as it describes Eisner's regime as CEO. The tale begins with Eisner's early successes rejuvenating Disney's live-action movie franchise and theme parks, the kickoff of the modern animation era with blockbuster hits like The Lion King and Beauty and the Beast, and the cultivation of a highly talented cadre of lieutenants, which reads like a Who's Who of executive talent now dispersed across the Fortune 500: Stephen Bollenbach (Hilton Hotels), Steve Burke (Comcast), Geraldine Laybourne (Oxygen Media), Richard Nanula (Amgen), Joe Roth (Revolution Studios), and so on. Stewart makes clear that Eisner has had a major eye for strong creative content himself, both as a young executive in his pre-Disney years at ABC and at Paramount Pictures and more recently in building partnerships like Disney's extremely lucrative one with Pixar.
Just as he credits Eisner for various Disney successes, though, Stewart assigns blame for the failures, too. The thoroughly researched 534 pages of DisneyWar make clear that his overall verdict on the CEO is negative. Much of the book describes detailed and specific interactions between Eisner and his rivals. Readers interested in the entertainment industry or in the personalities which drive it will not be disappointed. The blow-by-blow accounts of Eisner's feuds with Dreamworks SKG founder Jeffrey Katzenberg, who was his chief aide for nearly two decades, and Michael Ovitz, the superagent from CAA who had been friends with Eisner for even longer than that, are amazingly detailed. They show Eisner to be creative, funny, and charming when he wants to be--and devious, dishonest, and horribly Machiavellian when he doesn't.
Though dispassionate in his writing, Stewart assembles a withering portrait of Eisner as a grasping, self-centered, manipulative, and ultimately self-destructive executive. He shows how the Disney CEO has consistently undercut his potential successors within the company, in many cases drawing on Eisner's own writings and conversations with board members. He shows how Eisner's erratic attitude towards paying severance to former employees--in some cases being overly stubborn (as with Katzenberg, to whom he had a chance to close out for $90 million, but whom Disney ended up paying $280 million) and in others being shockingly lenient (as with Ovitz, who received a $140 million golden parachute after one relatively ineffective year at the company). He shows the overreach of grandiose projects like Euro Disney, and the missed opportunities like Lord of the Rings, Sopranos, and Survivor, on all of which Disney passed.
In the end, Stewart has returned with DisneyWar to what he does best: drilling into a murky and complex subject, capturing an enormous amount of detail through personal interviews, emails, memos, court records, and other data sources, and then weaving together a rich tapestry of people and events to bring others to the same conclusions he has clearly reached himself. Though some readers might tire of the reams of detail Stewart offers--at certain points, the book reads like a gossip rag, with intricate he-said, she-said accounts of individual meetings--most will enjoy it. Beyond the entertainment value, this book also has serious value to students of corporate governance, as it presents a scathing portrait of Disney's captive board of directors and shows what happens with the lack of proper CEO oversight. --Peter HanAbout the Author:
James B. Stewart is the author of the bestselling BLIND EYE, BLOOD SPORT and DEN OF THIEVES. Former page-one editor of The Wall Street Journal, Stewart won a Pulitzer Prize in 1988 for his reporting on the stock market crash and insider trading. He is a regular contributor to The New Yorker and SmartMoney.
Über diesen Titel kann sich auf eine andere Ausgabe dieses Titels beziehen.
Von Deutschland nach USA
Buchbeschreibung Simon & Schuster UK, 06.03.2006., 2006. Buchzustand: Gut. Auflage: New ed.. 608 Seiten in gutem Zustand B4123 ISBN 9780743496001 Sprache: Englisch Gewicht in Gramm: 378 19,6 x 12,8 x 3,0 cm, Taschenbuch. Artikel-Nr. 2160915
Weitere Informationen zu diesem Verkäufer | Frage an den Anbieter


text:
Daughters want Tammy Wynette's name on crypt
(Nashville, TN) -- Tammy Wynette's daughters are trying to get their mother's name restored on her burial crypt.
Georgette Jones Lennon says they agreed to have their mother's married name put on the crypt in 2012, but thought the change was temporary. Lennon and her sisters have started a public campaign and according to "The Tennessean," are talking to an attorney. The burial crypt at Woodlawn Memorial Park is owned by the daughter of Wynette's deceased husband.


text:
|Click photo to enlarge
Libby Cemetery
Libbytown, Stanstead County, Quebec
Contributed by Leslie Nutbrown, Nov 14, 2007 [email@example.com]. Total records = 9.
From Burroughs Falls take Route take Route 141 east toward Barnston. Turn right on Ways Mills Road. The cemetery is about 1 km on the left and sits back from the road.
You will have to park and walk across a field to get to the cemetery which you can see from the road. The cemetery dates from 1853-1902. Some stones were removed at one time and placed at Crystal Lake Cemetery. It is managed by the Barnston Heritage Cemetery Corporation.
I visited and recorded this cemetery on Nov 13, 2007, recording all existing stones and markers.
- Leslie Nutbrown
Griffin, Sylvanus, b. 6 Jul 1814, d. 19 Jan 1888
Libby, Charles C., d. 17 Jan 1894, age: 81yrs
Libby, Joshua, b. 7 Aug 1777, d. 18 Jun 1858
Libby, Lily M., d. 24 May 1887, age: 29yrs, wife of James McEachran
Libby, Olive M., b. 9 May 1819, d. ?, wife of Sylvanus Griffin, death date below ground
Libby, Sally, d. 1858, wife of Joshua
Libby, Sarah, d. 29 Oct 1873, wife of Benjamin Currier
Libby, Velara L., d. 16 Oct 1853, age: 12yrs
Miner, Martha L., b. 1817, d. 1902, wife of Charles C. Libby
Canada Obituaries and Death Notices
- Obituary Records, 1690-Today - GenealogyBank.com
- Find Death Records in Newspapers, 1690-Today - GenealogyBank.com
- Newspaper Funeral Notices, 1690-Today - GenealogyBank.com


text:
|130 m²
|Tipo de imóvel
|Estadia Mínima
|3 - 7 noites
Vistas deslumbrantes e sons do oceano, praia privada e do parque de estado adjacente. Absolutamente localização fabulosa que está muito perto de frente para o mar. Andar 50 passos para a praia orientada família privada. Relaxe na sua escolha de duas grandes decks de volta (um fora da sala de estar e um fora do quarto principal) com vista para o oceano e parque estadual. Couro coberto muito confortáveis sofás sala de estar. TVs de tela plana em sala de estar e quarto principal. Cozinha totalmente equipada. Sala de jantar separada. Campos de ténis e um pequeno parque infantil para crianças estão disponíveis dentro de uma curta distância a pé. Deck frontal proporciona uma vista agradável de amigos e familiares jogar tênis ou crianças que brincam no pequeno parque infantil. Desfrute de uma festa do caranguejo no pavilhão coberto ao lado do pequeno parque infantil. O estacionamento privado está na frente, e um quintal privado. Se você ama a praia, então você vai desfrutar completamente de nossa casa de praia.
Our three kids (all under 10) and our teenage niece all loved the townhouse. Plenty of room to be together.... or not. Very clean and comfortable. The full kitchen with a dishwasher made eating breakfast in easy. We enjoyed grilling out and eating on one of the decks with views of the ocean and dunes. View from the master bedroom was wonderful and peaceful. The outdoor shower and laundry area in the front entrance was great to keep the sand out of the house! Rental has beach chairs and umbrellas and is a short walk to the beach. Cute playground and tennis courts across the way that you can see from the front deck.
Beach is large with plenty of room to play beach games or just make sand castles. All but our youngest enjoyed the waves and boogie boarding! Sandy bottom and only waist deep for quite a way out. We saw dolphins every day!
The town of Bethany is cute but not much to do. We would not recommend The Frog House restaurant.
We enjoyed Rehobeth Beach (about 10 miles north) and their boardwalk. Bring lots of quarters for parking!! The boardwalk has an amusement park with something for all ages (even our 3 year old). We ate at Grotto Pizza two different nights. It is really good pizza, something that we could all agree on, and wasn't too pricey. We also had a great meal at The Summer House. We went before 6pm and missed the long line of families coming to eat.
We had fantastic crab cakes at the Fenwick House (on Fenwick Island a few miles south).
The property owners were very accommodating and helpful.
We would recommend this property to a friend.
We went with our 2 teenagers, toddler, and our active senior citizen mother who's in better shape then the rest of us combined. Everyone in our party had a lovely time at this house. The top deck has a simply lovely, expansive view of the ocean and dunes. The house is located in a quiet neighborhood and is comfortably decorated with plenty of sleeping space. It takes about a minute to walk to the beach so it's extremely convenient to go back to the house if needed. The kitchen is small if you have multiple people cooking, but it's well-equipped and serviceable. The owners were extremely responsive throughout our stay and thoughtfully provided plenty of games, puzzles, beach chairs & toys. There's a tennis court and nice playground just across the quiet street. Our family is ready to go back for another week here.
My entire family had a wonderful time at this beach house. It was just a short walk over the dunes to the beach, easily doable for our two kids under 5 yrs old. Convenient enough for easy trips back to the house for bathroom breaks or forgotten beach toys. We found the private beach uncrowded, clean and safe. The house was well-equipped for eating meals in, had plenty of living space (even for a larger family than ours) and the owners made available a closet full of beach chairs, umbrellas, and beach toys - as well as a cart to schlep it all to the beach. It was a quiet complex with lots of other kids, and a short drive to grocery stores, ice cream shops, and Rehoboth Beach's boardwalk and Funland - which our 2-1/2 yo and almost 5 yo LOVED.
Finally, the owners were always very prompt and courteous in returning my calls or responding to my emails. They couldn't have been easier to rent from.
Se você estiver olhando para injetar um pouco de emoção em uma praia tranqüila férias, em seguida, existem inúmeras atrações locais que estão disponíveis para o seu entretenimento. Bethany placa praia a pé é de 3 milhas ao sul da casa de praia. Tem de jantar fabuloso, compras e arcade. Ocean City é de 30 minutos a sul da casa de praia. Tomada de compras está disponível fora da rota 1. Escorregas de água, gaiolas de batedura, carros, golfe putt putt e atividades piscinas estão todos nas proximidades em uma instalação. Lojas de antiguidades está disponível. Golfe está disponível no prazo de 10 minutos. Supermercados e cinemas estão todos nas proximidades. Barcos de pesca em alto mar fretados estão disponíveis dentro de 10 minutos. Visite Fenwick Lighthouse Museum eo Museu Bethany Life Guard. Desfrute de um dia de vela, ou jet skis no iate clube nas proximidades.
|Validade da Tarifa
|por noite
|Noite (fim de semana)
|por semana
|por mês *
|Pacote ou Evento
jun 17 2017 - set 2 2017
Estadia mínima: 7 noite(s)
Early Fall
set 5 2017 - out 31 2017
Estadia mínima: 3 noite(s)
Tarifa Básica
Estadia mínima: 7 noite(s)
Trash pickup is Tuesday, Thursday and Saturday mornings. Trash receptacle is located to the right of the outdoor enclosed shower and it is located on the lower level. There are 2 Dune roads in Bethany; our beach townhouse is on the ocean side 1 mile south of the Indian Inlet River bridge. It is located in the Atlantic Watergate community; unit # 62.
The September rental period begins on 2pm of August 27, 2016.
* Tarifa mensal aproximada. O valor real dependerá dos dias em que você de fato se hospedar no imóvel.


text: Have you ever been frustrated by a computer errors? Now you can take revenge on all the dirty tricks your computer does! You can destroy a PC without any pain or cost. With the stress-relieving flash game METELE AL ORDENATA (Bash the computer) you are able to destroy that PC and relax.


text:
Boost your preparedness to an all new level!
Preparedness Advice Blog
Article Categories
Recommended by Noah
Give us a Facebook like!Facebook By Weblizar Powered By Weblizar
Recent Comments
Tag Archives: Now is a Good Time to Buy Precious Metals
Now is a Good Time to Buy Precious Metals
Posted on July 12, 2013 by admin
If you have your food storage and other preps in good order, now would be a good time to pick up some gold or silver as a hedge against inflation. Currently the Fed is printing $85 billion a month. There are two reasons the so called inflation rated is down, one the devious way in which the Fed figures inflation. Many factors that affect us are ignored. Second, the almost zero interest rates help keep the inflation rate down.
But look out when zero interest rates aren't enough to keep the economy moving, this will lead to inflation. If you look at history, inflation always returns it is just a fact of life, at some point you will face it.…Read More...
Posted in Self sufficiency, Uncategorized | Tagged Gold, Howard, junk silver, Now is a Good Time to Buy Precious Metals, precious metals, silver | 1 Comment


text:
Previous Issues
Northwest News ArchivesArchive
Inside IU Northwest is a monthly e-newsletter published by the Office of Marketing and Communications. The e-newsletter serves as a way for the campus community to stay connected and up-to-date on the latest IU Northwest news, announcements, and events.
Emergency 219-980-6501Facebook Twitter YouTube Flickr Instagram


text:
In today's Catholic Daily Roundup, top stories include anticipation of the August 6th 50 year anniversary of the Voting Rights Act, the GOP Presidential candidates' "Trump Strategy", and an outline of the moments that collectively signify a church that is "more open to welcoming the LGBT community and the diverse realities of the modern family than it was two years ago."
"The ultimate measure of a man is not where he stands in moments of comfort and convenience, but where he stands at times of challenge and controversy" (Martin Luther King)
Today's Gospel: "Martha, Martha, you are anxious and worried about many things. There is need of only one thing." (Luke 10:41-42)
In the News:
- August 6th is the 50 year anniversary of the Voting Rights Act which was signed into law in 1965. It eliminated literacy tests, other Jim Crow tactics, and prevented states from inventing new ways to suppress the vote. But over the past few years, many of the voting rights that the Voting Rights Act established as law have been slowly rolled back. Chief Justice John Roberts Jr. even declared that the Voting Rights Act had done its job, and it was time to move on.
- While many GOP Presidential candidates are preparing their messages for the American people in anticipation of the first Republican debate, they are also spending time preparing a "Trump strategy." According to campaign aides, figuring out how to handle Trump is subject they are constantly addressing with Presidential hopefuls.
- The University of Cincinnati Police Officer that was charged with the death of Samuel Dubose has been indicted for murder. After Samuel Dubose was pulled over for missing his front license plate, police officer Ray Tensing shot the unarmed man in the head.
Pope Francis and church news:
- In anticipation of Pope Francis' visit to the Capitol, House leaders are limiting floor access for former members of Congress. Proving just how much interest there is in the Pope's visit, House leaders moved to change the rules that govern access to the floor on Tuesday. Under the changed rules, only a limited group will have access to the House floor during the Pope's much anticipated speech.
- In TIME, Executive Director of Catholics in Alliance for the Common Good Christopher Hale outlines the moments and events that collectively reveal a church that is "more open to welcoming the LGBT community and the diverse realities of the modern family than it was two years ago."
- Leaders from the National Catholic Committee on Scouting expressed concerns over the Boy Scouts of America's new policy to allow openly gay troop leaders and employees to serve in their ranks at the national level. Although the new policy does not affect decisions about leaders made by local troops and councils and also includes a religious exemption, Catholic leaders are not happy. Nonetheless, the committee has urged Catholic churches to continue sponsoring Scout troops.
CACG would like to equip lay Catholics with the resources necessary to promote the common good in their communities. That's why we have the Catholic daily roundup. You can sign up for this short and digestible daily e-mail here.
We always appreciate your support to continue our work into 2015. Please invest in us today.


text:
For sale by Spoton Global Resorces VERIFIED 4 Aug 11:18 amAmuwo Odofin, Lagos
Available for sale is this Newly built 4-bedroom with a BQ fully detached duplex in Victoria Garden City Estate.
- The house is adorned with top-notch and high quality fittings/ fixtures
- spacious compound
- All room en-suite with guest's toilet at the entrance of the sitting room
- Separate Dining Area
- Family Lounge
- Pent house upstairs
- Very big and trendy kitchen with store
- high quality ward ropes
- En-suite boys quarter
- awesome lobby upstairs
- Located in a safe, secured and serene estate of VGC
Quite a Lovely Duplex you will Cherish..
Stay safe!
Watch out for:


text:
Pet Sitter in New York City
I absolutely love dogs and would be more than happy to work with them! I am eighteen years old and am studying jewelry design at the Fashion Institute of Technology in the Chelsea neighborhood. I am from Westchester County and had a shih-tzu with illness towards the end of his life so I am good with sick dogs as well.
Pet Sitter Details
|Email Address
|Phone Number
|Provider Type
Dog Walking
Pet Feeding
Pet Sitting
Notes made here will only be seen by you. If you want to message the owner of this listing
Jillian McDonnell's Other Listings
More in New York City, New York
Looking for Pet Sitter jobs in New York City, NY?
Find available jobs in your city.
Featured Pet Care Providers near New York City, NY


text:
2005-05-07 at 11:09:00 am #9208570 take Corvallis HP offer
Nearly 2,000 employees have taken severance package in
U.S. and Puerto Rico
In its biggest work force reduction in years,
Hewlett-Packard announced Tuesday that 570 Corvallis employees have accepted a
voluntary severance package and will be leaving the company over the next
several months.
Some 1,905 employees in the United States and Puerto
Rico decided to take the buyout offer, according to Monica Sarkar, HP's director
of corporate media relations. The company has not made public how many workers
were offered the package or exactly what kinds of positions are being
The job cuts are part of an effort to transform the
company's imaging and printing business, which has long been HP's most
profitable division. But Hewlett-Packard's market dominance has faced increasing
pressure from competing manufacturers and low-cost ink cartridge
Management told employees in January that the company wanted
to phase out some jobs in the division while adding others, and the buyout was
offered as a way of reaching job-cut targets without resorting to
The offers went out in early April, and employees had until
April 22 to make their decision. Those who opted to take the buyout will remain
on the job until anywhere from May 31 to Oct. 31, depending on the particular
projects they're working on, and the company is providing outplacement
assistance, financial counseling and other help during the
The severance package provides half a month's base pay for
each year of service with the company, plus two months' base pay. The minimum
payout is five months' base pay, with a maximum of 14 months' pay.
Sarkar characterized the number of employees taking severance locally as "a
positive response" to the offer, she didn't rule out the possibility of
additional layoffs. It's also possible the company would add some jobs in
imaging and printing locally, but Sarkar said that wasn't likely to happen in
the short term.
"That's something we're going to have to see. We're in
the process of balancing (the work force)," Sarkar said from the company's
headquarters in Palo Alto, Calif. "I think what you'll see is additional
adjustments, potentially additional reductions, depending on the business and
competitive climate."
Hewlett-Packard engineers invented inkjet printing
technology in Corvallis, which fueled rapid growth here through the mid-1990s.
But headcount peaked at 6,000 in 1996 and has been shrinking ever since. Today
the company employs a little less than 4,000 people at its sprawling Corvallis
campus, with the vast majority focused on inkjet or some other aspect of imaging
and printing.
While expressing the hope that employment at HP would
bounce back over time, Mayor Helen Berg acknowledged the cutbacks would have
wide-ranging repercussions, both economic and otherwise.
"This will be
felt in all sectors of the community," Berg said. "It's going to be felt in
retail, in the educational system, in all the businesses that provide
And because HP's employee base extends well beyond Corvallis,
she noted, the ripples will likely be felt all across the
"This will have an impact in Albany, in Lebanon and all
over," she said.
"Philomath, it's going to be huge there,
"Losing that many jobs is going to hurt," agreed Mysty Rusk,
president and CEO of the Corvallis-Benton County Economic Development
Partnership. But she added that even though the local economy can't absorb
nearly 600 unemployed workers, there are some tech-sector jobs
"We've got a number of small local companies that are in
situations that they could grow," she said. "They're seeing this as a real
opportunity to get some amazing talent."
And Corvallis has a long history
of nurturing startup ventures by HP veterans. Rusk said the EDP is scrambling to
put together a package of resources for entrepreneurial Hewlett-Packard
employees who might want to launch their own companies.
"We are going to
do our very best to start as many businesses as we can out of this," she said.
"This is an opportunity for us."


text:
Dress Loud for Global's Make Some Noise
Help raise money for Heart's new charity Make Some Noise by dressing loud!
Our new charity Make Some Noise is supporting worthy causes that help disadvantaged children and young people, like Y Bont in South Wales.
Get your workmates or school mates to join you in dressing loud! Just wear your loudest outfit and make a donation to help us "Make Some Noise" for charities that don't usually get heard.
Get involved and we could "Make Some Noise" by giving your business or school a shout out on air and Jagger and Woody, Kevin Hughes or our Heart Angels could be coming to you workplace or school on the day to join in the fun and collect your cash.
Our "Make Some Noise Day" is Friday 8th October, but you can organise a Dress Loud day whenever suits you!
Register to Bring a Pound to Work
Recently Played Tracks
To listen live, choose your preferred station:
Stay tuned...
Now playing: The best feel good music


text:
Spiritual Immersion Offer at Amankora Thimphu
Spiritual Immersion Offer at Amankora Thimphu
Consisting of five lodges dotted across Bhutan's pristine mountain terrain, Amankora offers unparalleled access to one of the world's most intriguing countries. This September, Dr Karma Phuntsho – Buddhist scholar, humanitarian and author of the first comprehensive English-language history of Bhutan – will guide a journey, both literal and spiritual, between the lodges, combining sightseeing with yoga, meditation and lectures. The tour includes all meals and drinks, as well as one 60-minute spa treatment.
COST: USD 14,880 for an 8-night itinerary based on two guests sharing a double room.
Spiritual Immersion Offer includes:
- Two nights each at Thimphu, Gangtey, Punakha and Paro lodges
- All meals, including picnics and in-house drinks
- Airport transfers
- Laundry and wifi
- Private vehicle/driver and English-speaking guide for internal transfers and sightseeing
- One 60-minute spa treatment per person, taken once during the journey
- Yoga, meditation and lectures
- All touring and monument passes
Terms & conditions:
- Available for stays between 13 and 21 September 2016
- Applicable for new bookings only
- The offer cannot be combined with any other promotional or group rates
- Subject to availability
Booking Period
04/04/2016 to 21/09/2016
Amankora Thimphu Information
- Location & Getting There
Amankora Thimphu rises majestically from a blue pine forest in the steep upper reaches of the Motithang area of the Thimphu Valley, just a 2-hour drive from Paro Airport.
- Accommodation
The 16 suites of the property comprise of a bedroom and an adjacent lounge, with a window banquette that provides fantastic views across a landscaped courtyard, stream or pine forest. The spacious bathroom features a terrazzo bath, which is separated from the bedroom with a traditional bukhari.
- Facilities
- Spa
- Outdoor dining deck
- Dining: 1 restaurant
- Activities
The hotel sits close to the capital's intruiging sights and shopping, and plenty of attractions and activities of the city. These include the National Textile Museum, Folk Heritage Museum and the bustling Changlingmethang Market. Archery, fishing, golf and white water rafting are also possible within and around the hotel.
- Children
Children are welcome but no specific facilities are available as the resort is somewhat better suited for couples.


text:
Unisource Inc
Thursday, July 24, 2014 from 5:30 PM to 8:30 PM (PDT)
Starting Business in China 101
China – one of the world's largest and fastest-growing markets – has been called "the business opportunity of the generation". But business in China is unlike business in any other country – China is different. Understanding how Chinese history, culture, and business practices affect China – Related ventures is essential if you are to maximize the opportunities and avoid unpleasant and costly pitfalls.
"STARTING BUSINESS IN CHINA 101" is an introductory session designed to provide you with an overview of the processes involved in starting business in China and the current opportunities available. You will leave this session with an understating of the steps you need to take to start your China business ventures and have an understanding of the current trends and needs in the Chinese Market.
The evening will include presentations from Two Key Note Speakers with first hand experience and knowledge of doing business with China "Names to be announced soon".
This program provides you with:
A framework for understanding Chinese business culture
Analysis of cultural values and behaviors prevalent in China
Discussion of the potential issues involved in establishing business in China such as;
policy, regulation, company registration, funding, etc
Practical strategies for working more effectively with Chinese counterparts
Who should attend?
Anyone considering or already:
Doing business with China
Establishing a network of Chinese suppliers
Experiencing the challenges of working in China
Light Dinner and Refreshments will be provided
Please feel free to contact us if you questions or would like to learn more about how to successfully grow your business in China.
firstname.lastname@example.com | (669) 231-0254 | 5201 Great America Pkwy Suite 232, Santa Clara, CA, 95054
We are a transnational business development company connecting high-tech U.S. companies to China's booming market, related industries, talents and government resources. We strive to build an ecosystem to support transnational projects including not limited to: industrial collaboration, joint venture,sales & marketing and talent acquisition.
Join millions of people on Eventbrite.
In order to purchase these tickets in installments, you'll need an Eventbrite account. Log in or sign up for a free account to continue.


text:
Does anyone know how I can ENABLE last user and "other user" on the Windows 7 logon screen? Computer is standalone. And I am not interested in using the "SpecialAccounts" key in the registry.
There's a zillion articles on the 'net on how to disable it, but nary an enabler...
Dec 8, 2011 at 6:19 UTC
Can't be done at this time in Windows 7 unless you add the machine to a domain..
Case closed.
9 Replies
Nov 19, 2011 at 2:50 UTC
For last user logged on to display:
Under administrative tools - Local security policy - local policies - security options:
Interactive logon: Do not display last user name
This security setting determines whether the name of the last user to log on to the computer is displayed in the Windows logon screen.
If this policy is enabled, the name of the last user to successfully log on is not displayed in the Logon Screen. ".
If this policy is disabled, the name of the last user to log on is displayed.
Default: Disabled.
Nov 19, 2011 at 2:55 UTC
In terms of the "other user" is there more than one local account? If so are the account(s) enabled? Experimenting myth my machine, I enabled the guest account and that made it an available account to log in with.
Nov 20, 2011 at 8:09 UTC
Yes, there are multiple accounts enabled on the machine.
Nov 20, 2011 at 9:21 UTC
Just trying to understand the "other user" issue Is it that there aren't tiles of the user accounts displaying or something else?
Nov 23, 2011 at 10:11 UTC
Just trying to understand the "other user" issue Is it that there aren't tiles of the user accounts displaying or something else?
Yes. The there are numerous "tiles" on the welcome screen. I am looking for the classic logon window that keeps the last user. If someone else logs in, they would click on "switch user" (rather than other user, sorry). The functionality that is enabled when Windows 7 is connected to a domain.
Dec 8, 2011 at 6:19 UTC
Can't be done at this time in Windows 7 unless you add the machine to a domain..
Case closed.
Dec 3, 2012 at 3:35 UTC
1st Post
This can be done... Add the registry key folder name ".DEFAULT" to HKLM\Software\Microsoft\Windows NT\CurrentVersion\ProfileList\
May 29, 2013 at 5:21 UTC
1st Post
Thanks JLeon. I was trying to enable a regular user who was not showing up on the login screen. used your method and now I can see that user. but this disables the administrator, but that is ok.
Aug 13, 2013 at 1:58 UTC
FixIT Chiltern is an IT service provider.
Does this work for Domain controllers and other servers running Server 2008 and 2008R2?
I just checked our DC and it is setup like Tom Alexander's first post, yet I only see "other user" when logging in via RDP. I ALWAYS have to type my username and password to log in. I would like to click on the last user user (me) and just type my password.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
I've made this soup a number of times and each time it has proved to be a satisfyingly hearty bowlful, particularly well suited to warding off the chills of late winter. It takes awhile to cook, but is absolutely worth wait!
Archive for the 'Mediterranean' Category
Lebanese Chicken and Mung Bean Soup
Posted in Chicken, Cilantro, Low-Fat, Main Course, Mediterranean, Mung Beans, Parsley, Rice, Side Dishes, Soup, tagged Chicken, Cilantro, Lebanese, Mung Beans, Parsley, recipe, Soup on 28/02/2009| Leave a Comment »


text: While you are taking Synthroid, you should avoid foods that can interfere with its absorption, thus making it less effective. These foods include walnuts, cotton seed meal, soy (including infant soy formula), and anything which is high in fiber. You should also avoid changing brands of levothyroxine or switching to a generic version if possible. This is because different preparations can work differently. If you must change, you should let your doctor know so that he can order blood tests to determine whether you are receiving the correct dose of the new drug.


text:
0 Rated
0 My Bookshelves
0 Recommendations
0 Notifications
Ruth Milkman
ISBN: 9780871546357 | Format: PaperBack | Release Date: 03/08/2006
Earn up to 330 points when you purchase this title.
Usually Dispatches in 4-5 days
Delivery Rates
Check your local Dymocks store for stock.
Please note that prices may vary between www.dymocks.com.au and Dymocks Stores.
Sharp decreases in union membership over the last fifty years have caused many to dismiss organized labor as irrelevant in today's labor market. In the private sector, only 8 percent of workers today are union members, down from 24 percent as recently as 1973. Yet developments in Southern California--including the successful Justice for Janitors campaign--suggest that reports of organized labor's demise may have been exaggerated. In L.A. Story, sociologist and labor expert Ruth Milkman explains how Los Angeles, once known as a company town hostile to labor, became a hotbed for unionism, and how immigrant service workers emerged as the unlikely leaders in the battle for workers' rights.
L.A. Story shatters many of the myths of modern labor with a close look at workers in four industries in Los Angeles: building maintenance, trucking, construction, and garment production. Though many blame deunionization and deteriorating working conditions on immigrants, Milkman shows that this conventional wisdom is wrong. Her analysis reveals that worsening work environments preceded the influx of foreign-born workers, who filled the positions only after native-born workers fled these suddenly undesirable jobs. Ironically, L.A. Story shows that immigrant workers, who many union leaders feared were incapable of being organized because of language constraints and fear of deportation, instead proved highly responsive to organizing efforts. As Milkman demonstrates, these mostly Latino workers came to their service jobs in the United States with a more group-oriented mentality than the American workers they replaced. Some also drew on experience in their native countries with labor and political struggles. This stock of fresh minds and new ideas, along with a physical distance from the east-coast centers of labor's old guard, made Los Angeles the center of a burgeoning workers' rights movement.
Los Angeles' recent labor history highlights some of the key ingredients of the labor movement's resurgence--new leadership, latitude to experiment with organizing techniques, and a willingness to embrace both top-down and bottom-up strategies. L.A. Story's clear and thorough assessment of these developments points to an alternative, high-road national economic agenda that could provide workers with a way out of poverty and into the middle class.
L. A. Story
Russell Sage Foundation
25.0 mm
152.0 mm
Sub Title:
Immigrant Workers and the Future of the U. S. Labor Movement
Management & Computers /
Publication Date:
228.0 mm
Be the First one to Review this Product
To read our fantastic eBooks, you will need the Free Dymocks eReader App.Select an option below:
I already have the Dymocks eReader App
Welcome to Dymocks, Australia's leading bookseller for 135 years.


text:
|Publication number
|Publication type
|Application number
|PCT number
|Publication date
|Jun 30, 1998
|Filing date
|Mar 18, 1995
|Priority date
|Mar 18, 1994
|Fee status
|Also published as
|Publication number
|David James Squirrell
|Original Assignee
|The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (15), Referenced by (18), Classifications (18), Legal Events (4)
|External Links: USPTO, USPTO Assignment, Espacenet
The present invention relates to a method and apparatus for monitoring a gaseous environment for the presence of cellular material; more particularly it relates to an apparatus that is capable of providing a measure of presence and/or numbers of cellular microorganisms, such as bacterial cells, in a large volume of air such as in a warehouse or production facility or in an open air location where bacterial presence is suspected. The method and apparatus of the invention are particularly provided for determining the likelihood of pathogenic or allergenic material being present in an environment by continuous on-line measurement of cell numbers. The latter format provides a continuous monitoring of an environment.
There is a military need for detection of the incidence of attack using biological materials, including attack using bacteria eg. in the form of cells or spores. Such need includes the capability to monitor the air some distance upwind of an asset site in order that sufficient warning may be given to personnel on that site that an attack with bacteria is imminent. In such circumstances it is required that monitoring be carried out continuously, that is for a continuous period of time for any one monitoring device eg. from several to several tens of hours.
There is a further need for determining the presence of pathogens in facilities such as hospitals and in manufacturing premises in which foodstuffs, sterile pharmaceuticals or physiological supplements are being placed in containers prior to use. Before a production run it is desirable that the sterility of the packing environment be checked for the presence of pathogens or less harmful bacteria that may be used as an indication of likely presence of pathogens or allergens.
In both of these situations it is necessary to process a large volume of air, either because of the continuous nature of the measurement or because of the need to sample a significant amount of clean room or sterile warehouse air. Furthermore in both situations it is necessary to screen for a wide range of bacteria, regardless of type, as the threat may not be that of a known genus or species.
It is known to use the luminol reaction to analyse air for the presence of haematin, but this technology is susceptible to giving readings with inorganic materials and is limited to a detection limit of 103 bacterial cells in theory as only 10-16 grams of haematin is extractable from the average bacterial cell. Metal sensitivity giving high backgrounds render this system unreliable in practice.
It is known to screen for the likely presence of bacteria by analysing samples for the presence of adenosine triphosphate. This is readily carried out using luciferase and luciferin agent whereupon the presence of ATP allows luciferase to catalyse the oxidation of luciferin with the resultant emission of light. Samples are loaded into a luminometer and the amount of light emitted used as a measure of the amount of bacteria present. In order to liberate as much ATP from any cells present it is known to add a detergent to the sample in order to lyse the cells and release the ATP.
Although such biochemistry has been extensively utilised with individual samples derived by direct sampling of surfaces, liquids and solids, there has been little development of luminometry equipment suitable for monitoring bacteria in air.
JP 62093634 discloses a counter for microorganisms which draws in an air sample, collects the microorganisms from that and extracts ATP from them before assaying the ATP using a luminescent reaction. This device uses a 0.2μ membrane filter to collect microorganisms from the air in a batchwise fashion, with the membrane being periodically analysed by being passed to an extracted station. No details of the sensitivity of this equipment are given, but its performance is limited by the ability of the air pump to draw sufficient sample air across the membrane and by the time taken to process the membrane microorganism content.
JP 58122281 discloses a method for detecting bacteria in air again using luminescent reagents to assay ATP. This method extracts ATP using a Tris-EDTA liquid buffer heated to 100� C. from samples of 10s of liters of air per minute batched in 10 minute samples. Filters are required to eliminate dirt and dust and these are described as essential to the method. A cooling tube is required in order to avoid increase of background noise due to raising of temperature of photomultiplier tubes use to monitor luminescence. This apparatus also uses an `extractor` to draw air into it at 10s of liters per minute. The exact nature of this `extractor` is not clear.
It is known to use cyclone devices to capture particulates from air, these devices typically being electrically driven and producing particulate depleted and particulate concentrated fractions. It is known to use such devices for the purposes of obtaining aerosols and other particulates from air for later analysis. For example GB 2245024 describes a cyclone for collecting a large sample volume of biological materials from the air; SU 1546481 and SU 11911460 describe use of cyclones to provide a particulate sample which is used to seed nutrient holding vessels or plates for analysis while SU 916535 collects bacteria from such cyclone on a filter band and viruses in a lower section wherefrom they are used to infect experimental animals. It is also known to use virtual impactors to collect airborne particulates, see eg. U.S. Pat. No. 4,942,297 and U.S. Pat. No. 4,670,135.
Again, none of these systems are capable of continuous monitoring of air for the presence of bacteria, particularly small amounts of pathogenic bacteria. A particular problem is the variation in concentration of fluid output from the cyclone with changes in humidity of the air being sampled. With very high throughput the cylone can run almost dry and produce high readings from a relatively normal background input.
JP 5184350 describes a system for counting of bacterial cells suspended in air which aims to shorten the determination time and improve the accuracy of the results. JP 60016598 describes an alternative device for detecting bacteria in an amount of air. JP 3112495 describes a filter system for the detection of different microorganisms floating in air. None of these devices are suitable for continuous operation.
The present inventor has now provided a truly continuous flow luminometry method and apparatus that are capable of continuous or batch monitoring of bacteria in a gaseous environment, particularly atmospheric air, such that on-line measurements may be taken of air bacteria content. Such apparatus is particularly directed at continuous monitoring but is equally suitable for sampling large volumes of air such as those inside a hospital, manufacturing facility clean room or sterile warehouse for sampling air before a production run.
The invention is further illustrated in the attached drawings in which
FIG. 1 is a diagrammatic representation of the invention;
FIG. 2 is a cross-sectional view of the cyclone and gas liquid interface as used in the apparatus of FIG. 1;
FIG. 3 shows a tubing element and reagent container network further described in Example 2; and
FIG. 4 is a graph in counts per minute output of the luminometer described in Example 1.
In a first aspect the present invention provides a method for determining the presence and/or amount of cellular material present in a gaseous environment comprising:
(a) continuously collecting a particulate fraction from that environment over a period of time;
(b) continuously transferring the particulate fraction to a processing fluid;
(c) continuously releasing intracellular contents including ATP from cellular material present in the processing fluid containing the particulate fraction:
(d) continuously adding luminescent reagents dependent upon presence of ATP to effect luminescence to the processing fluid;
(e) continuously measuring light emitted from the processing fluid produced in (d) in a luminometer wherein a signal indicative of this light is produced by the luminometer and the presence and magnitude of the signal is equated to presence and/or amount of such material present in the gas. Preferably the signal is analysed on-line by a operator or processing device, optionally at a site remote from the place where the method is being performed.
Preferably the gas is atmospheric air, the material is bacteria, and steps (b), (c), (d) and/or (e) are carried out continuously. Preferably step (b) is carried out using a detergent, but it may be carried out by use of heat, sound or other energy source or a lytic agent, eg an enzyme, with suitable cooling effect applied if excess heat has been generated.
In order to carry out all these steps continuously it is preferred to pass the processing fluid from the collecting step to the downstream steps using a conduit, whereby the time taken for bacteria collected in step (a) to be identified as such by their ATP content is limited only by the time taken for the fluid to pass down the conduit to the luminometry steps.
More preferably the passage of fluid down the conduit is controlled by a drive means such as one or more pumps whereby a constant flow of collecting fluid may be analysed by luminometry.
For the purpose of collecting the particulate sample it is preferred to suck air into a collecting device, deposit cellular material, eg. bacteria in the form of particulates into a collecting part of the collector device and discharge particulate depleted air from the collector. Such collection is preferably carried out at a rate of some tens to thousands of liters of air per minute in order that a useful sample is taken; conveniently at 100-1000 liters per minute.
In order to differentiate between bacteria and other cellular materials such as eucaryotic cells, eg. pollens, or fungal spores it is possible to split the particulate containing processing fluid into two streams, or use two collectors to produce two processing fluid flows, and add a detergent capable of releasing cell content including ATP from all cells and spores to one fluid flow and one that is only capable of releasing the content of one of the eucaryotic cells and fungal spores to the other. For such purpose it is possible to add eg. non-ionic detergent for releasing materials from eucaryotic cells and fungal spores and eg. cationic detergent for releasing it from all cells.
By subtracting the signal from the non-ionic detergent flow luminometer from that from the cationic detergent flow luminometer it is possible to produce a continuous signal indicative of bacterial presence and numbers.
In a still more preferred method of the invention bacteria are detected by detection of the amount of adenylate kinase activity in the processing fluid containing the collected particulates, wherein adenosine diphosphate (ADP) is added to the processing fluid and is converted by any adenylate kinase present to adenosine triphosphate which in turn is detected as described above. In this manner the sensitivity of the method is increased due to the cascade effect of the enzyme's activity leading to effective amplification of signal. Such a method when applied to bacterial detection in general is the subject of the applicant's copending application PCT/GB94/00118 by the same inventor. ADP may be included in the processing fluid as it enters the particulate fraction collection step, or may be added downstream eg. with any reagents added before the luminescent reagents.
The purity of the ADP added should preferably be such that the ADP to ATP ratio in the reagent is 2000:1 or more; more preferably 6000:1 or more. The concentration of ADP in the processing fluid may in theory be any level where it is in excess of the ATP already present in the organisms if significant sensitization is to be produced. It is preferred to use at least 0.01 mM or more ADP, more preferably 0.01 to 1 mM ADP or more as the final concentration in the processing fluid.
While cellular material contains sufficient magnesium for the magnesium dependent conversion of ADP to ATP, it will be realised by those skilled in the art that the ADP reagent should preferably be used in the presence of magnesium ions if ATP is to be optimally produced. Thus the ADP reagent is preferably made up in a buffer containing sufficient magnesium to provide magnesium levels at least as two or more times the molarity as the ADP. Preferably the conversion of ADP to ATP takes place in a buffer solution of pH 5.5-8.5 buffer, more preferably pH 7.8. The provision of magnesium is particularly preferred where ADP is stabilised using EDTA or like chelating agents.
It will be realised by those skilled in the art that the term continuously as applied to step (a) herein is intended to cover the collection of particulates continuously over an operating period.
Such period may be from several minutes to many hours or may be a set period of time typically prior to operation of a sterile processing line. For use in an interior sterile space this period will be sufficient for the apparatus to have collected particulates from a volume equal to a significant proportion of the air of that space, potentially substantially all of it, and may be collected in batches.
In a preferred form of the method wherein a sterile space such as a hospital building, clean-room or sterile packing installation is being monitored the method continuously collects particulates from the air while various vents supplying air conditioned air are operated or closed down. Processing fluid corresponding to the air collected during operation of each air vent and processing fluid collected with the conditioning system off are monitored using the on-line ATP measuring capability of the method of the invention, whereby a rapid indication of the location of any contamination is provided if any of the sets of fluid show increased bacterial presence. Where collection and processing fluid production is running for the entire period of test, as opposed to just collecting batches of particulates, it is possible to merely correlate the output of the luminometer at a particular time to the presence of bacteria.
In a second aspect of the present invention there is provided an apparatus suitable for carrying out the method of the present invention.
The apparatus of the second invention comprises:
(a) a means for continuously collecting a particulate fraction from a gaseous environment;
(b) a means for continuously transferring the particulate fraction to a processing fluid;
(c) a means for continuously releasing intracellular contents including ATP from bacterial cells or spores present in the processing fluid;
(d) a means for continuously adding luminescent reagents dependent upon presence of ATP to effect luminescence to the processing fluid;
(e) a light detector means adapted to be continuously fed with the processing fluid from step (d) and capable of emitting a signal indicative of the occurence and amount of luminescence detected thereby; and
(g) a signal transmitting means for feeding the signal from the luminometer to a processor and/or display means for indicating the presence and/or amount of bacteria.
The continuous collecting means (b) is conveniently provided in the form of a cyclone or a virtual impactor, preferably a high volume virtual impactor. Where continuous monitoring of external air, ie. outside buidings, is required it is preferred to utilise a cyclone, preferably one capable of processing between 100 and 1000 liters or more of air per minute and providing a particulate fraction therefrom on a continuous basis; no upper limit is necessary however as much higher capacity cyclones will be known to those skilled in the art. Preferably the cyclone is a wet walled hydrocyclone and the particulate fraction is provided in the form of a liquid processing fluid containing particulate as it leaves the cyclone. Where a limited volume of gas is to be sampled, such as in a sterile production line unit, it may be preferred to use a high velocity virtual impactor to remove particulates from the air; such impactor might conveniently be capable of sampling about 50 to 150 liters of air per minute.
With both of these options the means for transferring the particulate fraction to processing fluid preferably comprises a supply of processing fluid into which the fraction is deposited. The processing fluid is preferably a liquid such as, water or a buffer, optionally containing magnesium ions, ADP and/or reagent for releasing intracellular contents including ATP from cells.
Where large volumes of gas are being sampled with variation in humidity it is preferred to add any detergent downstream of the collector and use a gas liquid interface that is capable of maintaining the dilution of the particulate in the processing fluid at a substantially constant level and removing excess air as bubbles. One suitable gas liquid interface is that described in copending application EP-A-668095.
A preferred modification of this interface receives liquid processing fluid under influence of a pump between the collector and interface. When the liquid level in the interface falls below a set level a level sensor transmits a signal to the pump to cause it to increase fluid flow rate. At the same time air entrained as bubbles in the liquid is allowed to escape to the atmosphere thus ensuring a reasonably constant supply of liquid to the downstream stages of the apparatus.
A further modification of this device places the pump downstream of the interface, and the pump is slowed when the interface liquid level falls below the set level such that the liquid level might recover.
The flow rate of processing fluid through the cyclone may be any flow to which a suitable amount of detergent, ADP, phosphate and luminescence reagents can be supplied without logistical problems. A convenient flow rate is from between 0.1 and 10 ml processing fluid per minute where 1000 liters of air minute is entering the collector, eg. cyclone, at the same time.
The means for releasing intracellular content including ATP may include a heater, sonic device or device for adding a lytic agent such as an enzyme or detergent as described above in the description of the method. Where the means relies on physical effect to release cell content it must produce that effect at or downstream of the means for transferring particulates to the processing liquid. Where the means adds a lytic agent it may be positioned upstream, at or downstream of the transferring means. A cooling device may be included before the downstream luminescence steps if heating is used.
The processing fluid is passed from its supply, ie. a reservoir, through the particulate transferring means and to any downstream intracellular contents releasing means by means of a fluid flow path. Preferably this is provided in the form of a liquid conduit, more preferably in the form of tubing. The processing fluid is preferably liquid moved through the conduit by means of one or more pumps and preferably these are peristaltic pumps which act upon the liquid through the conduit wall; preferred conduits being of flexible tubing type, more preferably flexible transparent plastics tubing suitable for delivering liquid under the influence of peristaltic pumps.
Using flow rates of from 0.1 to 10 ml processing fluid per minute to the cyclone, it may be expected that evaporation will reduce flow still further such that for example 0.05 to 10 ml per minute, but preferably 0.5 to 5 ml per minute, will enter the interface. At such flow rates the apparatus can conveniently use peristaltic tubing of the order of 0.25 to 3 mm internal diameter, although no particular limit is placed here other than the fact that the tubing should not be too wide to retain an air free flow through the apparatus. Suitable silicone rubber tubing for this purpose is available from Autoclude at 0.8 mm internal diameter; alternative tubing is available from Watson Marlow-UK or Ismatec-Switzerland.
Where a lytic agent is added downstream of the particulate transferring means a junction is preferably provided whereby a single peristaltic pump acts upon one or more tubes carrying processing fluid from the transferring means and one or more tubes carrying the lytic agent, preferably a solution of lytic agent; at least one of each type of tube feeding into a single downstream tube at a junction between the two.
A similar arrangement, preferably with a separate peristaltic pump, is provided for the optional addition of any ADP reagent in the adenylate kinase based aspect of the method whereby enhanced amounts of ATP are derived from a given number of bacteria and similarly for the means for adding luminescent reagents. In the latter case the junction between the conduit carrying processing fluid from step (c), with the conduit carrying luminescence reagents is preferably effected in the light measuring device itself, eg. a luminometer light measuring chamber.
Where the liquid processing fluid plus particulates is being divided into two flows for processing with different lytic agents it is possible for these to be produced by separate cyclones or impactors, at the processing fluid outlet of a single cyclone or impactor, at the air liquid interface or downstream of these. One embodiment of the apparatus of the invention provides these streams by use of a manifold downstream of the interface, and preferably uses this manifold for mixing the two lytic agents with respective ones of these streams. Thus the manifold has typically a central inlet from the interface carrying for example approximately 50% of the liquid flow input and preferably 80%. to the collector (dependent upon evaporation in the cyclone or impactor) and that is flanked by respective inlets from supplies of the two detergent reagents used at eg. 25% of that central inlet flow each. Two outlets from the manifold provide combined flows made up of 50:50 mixes of the central flow and the respective detergent flow. Typically this might be of the order of 75% to 125% of volume of the flow from the interface from each of the manifold outlets.
In a preferred embodiment of the apparatus of the invention, particularly suited to outdoor monitoring such as would be required in protecting a military asset, it is preferred to have two flows of processing fluid and treat these with different intracellular contents releasing means in order to distinguish between eucaryotic cells and fungal spores on one hand, and bacteria on the other. These flows may emanate from a single particulate collector or from two separate collectors operated at the same location. The preferred detergents used for such flows are those as described above for the method of the invention and as disclosed in PCT/GB94/00118.
In an alternative embodiment of the invention one of the luminescent agents, preferably luciferase, is immobilised near the luminometer light detector within the light measuring chamber where the processing fluid and luminescence reagents, in this case containing luciferin solution, are mixed. By being immobilised near the detector the efficiency of the detection of light may be maximised. In order to maintain the activity of the luciferase this may be fixed on a ribbon like substrate that is wound from one reel to another such that fresh luciferase is presented to the incoming fluids at a desired rate. Immobilisation may be achieved by chemically or physically linking the enzyme, eg. using a chemical linker group such as glutaraldehyde, to a suitably derivatised planar substrate, and the liquid tight nature of the chamber may be retained by introducing the ribbon of this substrate through eg. rollers that are liquid tight or are provided above the liquid level.
It will be realised that the method and apparatus of the invention may operate at ambient temperatures, but may also be operated at higher temperatures where reagent heat stability so permits. Thus the tubing or the luminometer light measuring chamber may be heated in order to increase the amount ATP produced by the ADP agent or the light emitted from the luminescent reagents in response to presence of ATP. For such use the thermostable luciferases of the applicant's copending GB applications, British Patent Application No. 940570.2, 9501170.6 and 9500660.7 (which were superseded by PCT/WO95/25780) may be used.
It will be realised by those skilled in the art that after exiting the luminometer chambers, eg. by further peristaltic tubing, the flows may be forwarded to further analytical devices such as those using specific binding to more specifically determine the nature of any bacteria present, or other agents such as viruses, DNA or chemical agents.
Further provided by the method of the invention is a tubing element or network of such elements suitable for use in the apparatus of the invention comprising a peristaltic tubing element or network of such elements characterised in that the element or network has a first tubing length with a free end suitable for attachment to the fluid outlet of an apparatus for continuously collecting a particulate fraction from a gaseous environment, preferably a gas liquid interface of such device, a junction between the other end of the first tubing length and a second tubing length, the second tubing length being suitable for connection to a source of reagent, such as a reagent container, a further tubing length provided leading from the junction of the first and second lengths having a free end suitable for connection to an inlet means of a luminometer chamber; all the lengths being capable of peristaltic action under influence of a peristaltic pump.
Preferably the element or network of the invention comprises one or more further junctions between the junction between the first and second tubing lengths with lengths of tubing having free ends suitable for connection to further sources of reagents.
Thus where detergent is used to lyse cellular material and is added upstream of the particulate collector, and bacterial ATP is being measured directly, a tubing element merely joining the interface and luminometer light measuring chamber may be used. Where the detergent is added downstream of the collector and the adenylate kinase variant of the method is being employed two junctions will be required to allow linkage with lengths of tubing from a detergent and ADP reagent source respectively. A separate luminescent reagent source and tubing may be included with the element or network as part of a reagent replenishment kit.
In each case it is preferred, by the very nature of the apparatus, that the free ends of the tubing, including those leading to reagent bottles, be covered to exclude the entry of bacteria and other cellular materials, preferably by a puncturable or removable end cover, eg. removable by tearing, cutting or pulling. Still more preferably the free ends to the reagents are actually attached to the reagents required such that a sterile replacement tubing network might be replaced all in one go with the amounts and concentrations of the various reagents being matched to each other such that they last similar lengths of operation time.
The method, apparatus and tubing element and networks of the present invention will now be exemplified by way of illustration only by reference to the following non-limiting Examples and Figures. Further embodiments of the invention falling within the scope of the claims will occur to those skilled in the art in the light of these.
FIG. 1 shows a diagrammatic representation of an apparatus of the invention comprising a cyclone and gas-liquid interface feeding twin lines with non-ionic and cationic detergent feeds, luminescence reagent feeds, luminometers and a central processing unit.
FIG. 2 shows a cross section through a cyclone and gas liquid interface as used in the apparatus of FIG. 1.
FIG. 3 shows a tubing element and reagent container network suitable for replacement of reagents as one unit for an apparatus as described in Example 2.
FIG. 4 is a graph of a counts per minute output of a luminometer as described in Example 1 when various amounts of ATP are added into the tubing directly downstream of the air/liquid interface.
A continuous flow luminometer apparatus of the invention was constructed using a cyclone unit (1), capable of removing particulates from approximately 1000 liters of air per minute using water as the processing fluid, connected to a gas liquid interface device (2) downstream for degassing the fluid and splitting the flow into two parallel processing flows. In this manner the liquid collects the particulates, including any aerosols, and carries them under influence of action of peristaltic pumps (4) via peristaltic tubing conduits (3) of 0.8 mm silicone rubber (Autoclude) to junctions (5) where the influence of pumps (5) draws a flow of detergent (either 0.2% aqueous CTAB solution or 0.4% aqueous Triton X-100) from containers (6) into the processing liquid flow (giving a flow concentration of 0.1% CTAB or 0.2% Triton X-100). Further pumps (7) draw the fluid flows on and deliver them at the same rate as a solution containing a flash kinetic mixture of luciferase, luciferin and buffer (Biotrace plc Bridgend, UK) from containers (8) to a light measurement chamber (not shown) within a luminometer housing (9) where the respective flows and reagents are mixed. The pumps (7) achieve synchronous delivery by acting upon delivery lines (10) and (11) simultaneously and the relative amounts of ATP released by the same particulate sample under influence of the detergents provides amounts of light and thus signals indicative of total cells/spores and eucaryotic cells/spores.
The cyclone is shown in more detail in FIG. 2, where (12) is an inlet for gas (atmospheric air) to be separated into particulate enriched and particulate depleted fractions, (13) is a supply of water processing liquid pumped at 1 ml/minute which is entrained with the air flow and passes therewith into the cyclone body main volume (14) where particulate depleted air exits under influence of an air mover (15) while processing fluid and entrapped particulates pass downward to an outlet (16) in the bottom of the cyclone. A peristaltic pump (17) passes the processing fluid to a gas liquid interface of capacity approx. 100 μl where bubbles are removed; the rate of supply of the processing fluid by the pump, or alternatively the removal of fluid by a pump downstream, being determined by the liquid level in the interface. Liquid level is determined by a liquid level sensor (not shown) and where the level falls below a set height the pump is operated to draw more processing liquid through until the desired level is restored. Excess liquid and dense particulates from the interface are removed periodically from outlet (18), air leaving the liquid as bubbles is removed via outlet (19) and processing liquid is passed under influence of a peristaltic pump (20) to the downstream tubing for addition of reagents.
As the processing liquid with particulates and detergent mixes with the luminescent reagents in the luminometer light measurement chamber, a light sensor (not shown) measures any light emitted in the chamber resultant from the presence of ATP; emission being rapid due to the flash kinetic ratio reagent having excess luciferase compared to luciferin over the ratio required for glow kinetics (where measurement is over several minutes). The luminometer transmits a signal representative of light emitted to a computer processor which in turn may display this on a display unit or compare it with control levels.
The detergents used may be standard chemicals as described above but may be provided in the form of specialist proprietary extractants such as Enzymatics ATP releasing agent, Biotrace XM extractant (Biotrace, Bridgend, UK) or Lumac NRM (Lumac BV Holland). The luminescence reagents may be standard reagents available from suppliers such as Biotrace plc and Celcis plc UK and others; the luciferase may be natural or recombinant. Concentrations of reagents are those necessary to produce flash kinetics and their ratio to the amount of cetyl trimethyl ammonium bromide treated processing liquid is that which the manufacturers recommend for batch processing taking into account the relative sizes of the tubing from each line entering the luminometer chamber. The speed of peristaltic pumps may be also adjusted to maintain ratios where separate pumps supply each flow.
A second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow is included for feeding very pure adenosine diphosphate (ADP) reagent, with respect to ATP, into the processing fluid downstream of the junction (5) where detergent is added, but upstream of the luminometer. The ADP reagent (99.95% pure with respect to ATP) is added at a flow and concentration such as to produce a concentration of 0.5 mM in the final flow. The processing fluid is pH 7.4 phosphate buffered saline or TrisHCl pH 7.8 including 0.2 mM magnesium ions.
A tubing/reagent replenishment pack provided for the apparatus of Example 2 is shown in FIG. 3. All tubing is of silicone rubber of 0.8 mm internal diameter joined at durable resilent plastics junctions dimensioned to receive them. The tubing has puncturable sealed ends for connection to one of the interface outputs at a first end (21) and to the luminometer light measuring chamber inlet at the other (22). Container (6) holds detergent reagent and has a sealable air inlet for allowing compensating air to enter as reagent is drawn out under influence of the peristaltic pump (as 4 in FIG. 1) on the tubing from the container to junction (5); this pump simultaneously acting upon tubing (3). A further piece of tubing connects junction (5) to junction (5a) where a tube from a second reagent container (6a) carries ADP reagent under influence of a further peristaltic pump (not shown in FIG. 1). The tubing between junction (5a) and the end (22) is acted upon by a still further peristaltic pump (as 7 in FIG. 1) which also acts upon a tube feeding luminescent reagent to the luminometer chamber.
In preferred apparatus the flow cells are included in the tubing pack and are also disposable; these being made of polycarbonate or polystyrene.
The amount of ATP present in a typical bacterial cell is of the order of 10-18 moles, equal to about 10-15 grams (see Lundin A (1989) ATP Luminescence-Rapid Methods in Microbiology ed. Stanley P E et al; Blackwell, Oxford pp 11-30; and Stanley P E (1989) J. Biolumin. Chemilum, 4, pp 375-380). Levels may be expected to be reduced in aerobic organisms when deprived of oxygen and the intracellular concentration may vary typically between 2 and 10 mM.
Calibration of the continuous flow luminometers of Example 1 and 2 may be carried out by placing known amounts of ATP into the processing fluid or cyclone while the apparatus is operating and constructing a calibration curve by plotting counts per minute of the luminometer light detector output vs amount of ATP (see FIG. 4). Alternatively known concentrations of bacteria such as Bacille Camille Guerre or E. coli may be aerosolised at a set distance, eg. 1 to 10 metres, away from the cyclone inlet and these amounts then plotted against luminometer output as before. Both luminometer light measurement chambers will be exposed to similar amounts of ATP where ATP is used to calibrate, whereas the non-ionic detergent chamber will receive significantly less where bacteria are used; thus a calibration using ATP and bacteria and perhaps eucaryotic cells or fungal spores may be preferable to determine all is operating as it should.
In operation the cyclone supplies particulate sample to the interface from where it is separated into two flows of equal volume and rate by the manifold, each flow containing one of the two detergents, non-ionic or cationic. The flows are mixed with the appropriate amount of ATP/phosphate reagent as required and pass to the luminometer light measurement chambers where luminescence reagents are mixed with them and light emitted. These reagents are preferably of flash kinetic type and allow almost instant emission of light which is detected by light sensors associated with the chambers which in turn pass electrical signals indicative of the amount of ATP detected to a computer processor where the two signals are used to determine the difference in signal, thus ATP, and thus bacterial cells and spores, between the two flows; this being carried out by producing an output to a display unit or printer indicative of raw luminometer output or, using software, a direct estimation of bacteria present as derived by reference to calibration curve stored in a processor associated memory.
The whole operation of the pumps may be controlled by the processor in accordance with preprogrammed regime. Thus if conditions are very dry it might be desired to alter the cyclone rate of air collection or the rate at which sample is passed to or through the interface. Alternatively supplementary collection fluid may be added at the cyclone to dilute the sample if required. Other controls involving rate of operation of one or all of the peristaltic pumps and rate of movement of tape driven immobilised luciferase may also be so controlled as will be appreciated by those skilled in the art.
The preferred apparatus of the invention as shown in FIG. 2 uses feedback from the interface to control liquid addition to the cyclone.
|Cited Patent
|Filing date
|Publication date
|Jan 9, 1978
|Sep 4, 1979
|Pye Limited
|Apparatus for the detection of volatile organic substances
|Oct 4, 1966
|Dec 9, 1980
|General Electric Company
|Nuclei analysis of solid substances
|Jun 27, 1980
|Feb 2, 1982
|Chevron Research Company
|Method for removing coke from particulate catalyst
|Sep 30, 1982
|May 15, 1984
|The United States Of America As Represented By The Secretary Of The Air Force
|Method for the particle size independent spectrometric determination of metal particles in lubricating oils and hydraulic fluids
|Feb 19, 1986
|Aug 25, 1987
|Washington Research Foundation
|Virtual impactor
|Nov 25, 1985
|Dec 27, 1988
|Liquid Air Corporation
|Method for measurement of impurities in liquids
|May 1, 1989
|Apr 2, 1991
|Lenon Envionmental Inc.
|Sampling processes for use in the controlled addition of conditioning material to suspensions, sludges and the like and apparatus thereof
|Jan 22, 1990
|Sep 10, 1991
|Board Of Regents, The University Of Texas System
|Processes for removing sulfur from sulfur-containing gases
|Oct 19, 1992
|Jan 18, 1994
|Rupprecht & Patashnick Company, Inc.
|Carbon particulate monitor with preseparator
|Dec 28, 1993
|May 23, 1995
|Eastman Kodak Company
|On-line method for measuring density of solids in reaction process
|Feb 6, 1995
|Jun 25, 1996
|Ahlstrom Machinery, Inc.
|Gas sparged hydrocyclone with foam separating vessel
|Jul 15, 1983
|Feb 22, 1984
|Packard Instrument Company, Inc.
|Method of concentrating and measuring unicellular organisms
|May 15, 1984
|Nov 21, 1984
|Packard Instrument Company, Inc.
|High resolution method of measuring ATP, and concentrating and measuring unicellular organisms
|Title not available
|Title not available
|Citing Patent
|Filing date
|Publication date
|Apr 21, 1998
|Jun 29, 1999
|The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britian And Northern Ireland Of Defence Evaluation Research Agency
|Cellular material detection apparatus and method
|Sep 28, 1999
|Aug 15, 2000
|The United States Of America As Represented By The Secretary Of The Navy
|Cyclone aerosol sampler and biological aerosol chemiluminescent detection system employing the same
|Aug 25, 2000
|Jun 11, 2002
|The Regents Of The University Of California
|Low pressure drop, multi-slit virtual impactor
|Aug 21, 2000
|Feb 11, 2003
|Larry Don Robertson
|MBI vortex bioaerosol cassette insert
|Mar 29, 2001
|Nov 25, 2003
|Neogen Corporation
|Apparatus and method for chemiluminescent assays
|May 28, 2004
|Dec 11, 2007
|Shoaf Antony R
|Endospore detector
|Oct 1, 2004
|May 13, 2008
|The United States Of America As Represented By The Department Of Health And Human Services
|Air-sampling device and method of use
|May 12, 2008
|Jun 26, 2012
|The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention
|Air-sampling device and method of use
|Oct 1, 2004
|Mar 29, 2007
|Chen Teh-Hsun B
|Air-sampling device and method of use
|Oct 19, 2005
|Dec 11, 2008
|Medical Innovations International, Inc.
|Rapid and Sensitive Detection of Bacteria in Blood Products, Urine, and Other Fluids
|Dec 6, 2014
|Apr 9, 2015
|Industry-Academic Cooperation Foundation, Yonsei University
|Apparatus for measuring floating microorganisms in a gas phase in real time using a system for dissolving microorganisms and atp illumination, and method for detecting same
|Sep 9, 2010
|Mar 15, 2012
|Eurofins Wej Contaminants Gmbh
|Vorrichtung zur Entnahme einer repr�sentativen und zerst�rungsfreien Probe von Partikeln aus Sch�ttgut sowie Verfahren zur Entnahme mittels der Vorrichtung
|Sep 9, 2010
|Jun 6, 2012
|Eurofins Wej Contaminants Gmbh
|Verfahren zur Entnahme einer repr�sentativen Probe von Partikeln aus Sch�ttgut zur Bestimmung einer Mykotoxinkontamination des Sch�ttguts
|Jan 10, 2011
|Jul 13, 2011
|Hitachi Plant Technologies, Ltd.
|Inspection method of airbone floating bacteria and its apparatus
|Dec 1, 2010
|Nov 28, 2012
|Hitachi Plant Technologies, Ltd.
|Apparatus for trapping floating bacterial cells, method for counting floating bacterial cells, and system for counting floating bacterial cells
|Dec 1, 2010
|Jul 9, 2014
|Hitachi Ltd
|Apparatus for trapping floating bacterial cells, method for counting floating bacterial cells, and system for counting floating bacterial cells
|Sep 28, 2007
|Sep 18, 2008
|U.S. Genomics, Inc.
|Air sampler
|Sep 28, 2007
|Nov 13, 2008
|Us Genomics Inc
|Air sampler
|U.S. Classification
|International Classification
|Cooperative Classification
|European Classification
|Feb 5, 1997
Owner name: SECRETARY OF STATE FOR DEFENCE IN HER BRITANNIC MA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:SQUIRRELL, DAVID JAMES;REEL/FRAME:008484/0828
Effective date: 19970122
|Nov 19, 2001
|Fee payment
Year of fee payment: 4
|Nov 17, 2005
|Fee payment
Year of fee payment: 8
|Dec 24, 2009
|Fee payment
Year of fee payment: 12


text:
- Body Description
- AWD 4dr SUV
- Exterior
- Starfire Pearl
- Interior
- Sepia Nuluxe With Linear Espresso Trim
- Transmission
- 6-Speed Automatic
- Drive Train
- Stock#
- 48165
Expires 07/31/2017. Excludes official fees, taxes and dealer charges. No security deposit required. 10,000 mile/year limit.
*Available on approved credit to very well qualified customers through Lexus Financial Services and participating Lexus dealers on a new 2017 GX 460. Not all customers will qualify. Offer based on MSRP of $58,480 including delivery, processing and handling. Monthly payment may vary depending on final price of vehicle & your qualifications. You pay $0.25 per mile over 10,000 per year. See dealer for vehicle and lease program details. Must take delivery by 07/31/2017. This offer is available in CA, NV, AZ, NM, CO, UT, ID, OR, WA, AK.


text:
When you have had a stroke you are at increased risk of having one again. Yikes! I see patients that have had strokes or TIAs (transient ischemic attacks, or mini-strokes) that have no clue they have the power to control their own stroke risk.
\r\n

\r\n
Here are 5 quick tips or goals that everyone should know to help prevent a stroke.
\r\n

\r\n
1. Blood Pressure: Goal is less than 120/80 mmHg
\r\n

High blood pressure slowly does damage to our arteries that feed our brain
\r\n
I suggest each home have a blood pressure machine. These relatively inexpensive machines allow you to check and track your blood pressure more frequently so you can stay on top of it.

\r\n
2. Cholesterol: Goal is LDLs less than 70 mg/dL (LDLs are the bad cholesterol)
\r\n

High cholesterol clogs up our arteries with plaque and other junk which leads to stroke and heart disease.\r\n
\r\n
This can be checked with a simple blood test while fasting.\r\n
\r\n
Diet and exercise can help lower your cholesterol and LDLs (bad cholesterol), while also increasing your HDLs (good cholesterol).

\r\n\r\n
3. Diabetes: Glucose Goal is 70-110 mg/dL
\r\n

It is not uncommon for diabetics to be completely unaware that they have diabetes.\r\n
\r\n
A blood test called an "A1c" is commonly used to get an overview of what a person's blood sugars have been over a three month period, and is a good indicator for diabetes. Your A1c should be below 7 percent. An A1c of 5.7 percent or higher can indicate you may be at increased risk for developing diabetes. \r\n
\r\n
 Diabetes damages and ages the entire body, especially the brain and heart.

\r\n
4. Diet: Goal is low fat, low salt
\r\n

Stay away from deep fried foods, fast foods, and use olive oil instead of butter or margarine when you cook. The monounsaturated fat in olive oil is one of the best types of fat to use in cooking.\r\n
\r\n
Get rid of your table salt and learn to watch for sodium on the food labels.\r\n
\r\n
Eat at least five fruits and vegetables per day.\r\n
\r\n
Choose whole grains when buying breads, cereals and pastas. Whole grains don't leave anything out.\r\n
\r\n
Choose feathers and fins over high-fat (red) meats. Poultry and fish are excellent lean sources of protein. One great choice is salmon, which is rich in omega-3 fatty acids that may help protect you against stroke and heart disease.

\r\n
5. Exercise: Goal is 150 minutes per week
\r\n

Exercise should be at a moderate level. This is a level where it becomes hard to carry on a normal conversation but you are not completely out of breath. This is a good aerobic level.\r\n
\r\n
To reach your exercise goal you should exercise 30 minutes a day, 5 days a week.\r\n
\r\n
Walking, biking, hiking, dancing are all good examples of aerobic exercise.

\r\n

Of course, in addition to these quick tips you need to not smoke or abuse alcohol or drugs. Assuming you do not have any of these unhealthy habits, following these five quick will put you well on your way to stroke prevention.
\r\n


text:
Reloading dies are expensive, and once you get them set up it's nice to store them so they won't be disturbed. The MTM Die Storage Box hold for 3-Die Sets of rifle or pistol dies (each die in its own molded recess in the upright position). There is also space in the box for a dummy round loaded by each die set and the shellholder for each die set. This box is not tall enough for the Redding Competition seaters for the Forster Ultra Seaters. Available in red only.
Mfr Part: DB430
Color: Red
Would you like to be alerted when the item becomes available again?
Please enter your email address and quantity below andwe will send you an alert when the item becomes available.
Please note that alerted inventory is on a first come, first serve basis.
Thank you for contacting our Chat Team! Please visit us again.
If you would like a copy of the chat transcript please click "Send Transcript" below.
Hello, to begin a chat session with a customer service representative, complete the fields below and click Chat


text:
Amy Clatworthy Daigle
Director, Operations
As the Director, Operations, Amy's role at Common Impact includes working with our corporate partners, as well as evolving our operational systems for innovation and growth. Amy began her career at Fidelity Investments. Through her volunteer work, sponsored by Fidelity Investments, she transitioned to the nonprofit sector. She knows firsthand how corporate engagement programs can impact our communities. She has over 15 years of nonprofit experience working at local, national and international nonprofits. Previously, she was the Director of Special Projects, at the National Center on Family Homelessness. Most recently she served as the Director of Operations and Systems Infrastructure, at Facing History and Ourselves, an international educational and professional development nonprofit.
Amy has a BA in Communications from The College of Wooster and a Masters in Nonprofit Management from Case Western Reserve University.


text:
Our Family of Schools
With a large and diverse portfolio of schools, Cognita draws upon our international breadth and wide-ranging educational expertise to share best practice across our Group.
To achieve more than you believe possible – that's the hallmark of a quality education. At Cognita, it's what we strive for within each of our schools. It's what we want for our students, for our parents and for the people who work with us.
Drawing upon our group of schools and regional offices around the world, we offer outstanding opportunities to develop your career. We want to help you achieve more, too.
We're growing all the time. Cognita is an international group employing more than 5,000 teachers and professional staff between our 68 schools, UK head office and regional offices in Europe, Latin America and South-East Asia.
Teaching excellence represents everything we stand for at Cognita. We're committed to hiring and retaining outstanding talent: teachers and school leaders who are passionate about their craft as well as functional leaders and professionals in our head and regional offices who can take the business forward.
We're looking for people who want to make a positive difference to children's lives, who can help us stretch and support children to achieve beyond expectation.
Our diverse portfolio of schools and regional offices, combined with our ongoing investment in the professional development of our people, means we can offer first-class career opportunities with a global dimension. If you want to take your career further, we want to support you in achieving that goal within Cognita.
Want to be the first to hear about Cognita Jobs?
Sign up to our jobs newsletter now
With a large and diverse portfolio of schools, Cognita draws upon our international breadth and wide-ranging educational expertise to share best practice across our Group.


text:
Discover a selection of top forums dorinta cracked on the internet. Come to share your passion on forums dorinta cracked and meet other fans of dorinta cracked. Then, why don't you create your own forum of dorinta cracked ?
A forum made for Zidocraft - Minecraft Server Community!
zidocraft, minecraft, #cracked, server, free, hamachi, whitelist, paying, donator, perks, mcmmo, vanish, disguise, craft, logblock, griefing, multiverse, premium, survival, 24/7
We are Xbox 360 and PS3 modders! We love to have fun and make people smile with mods!
xbox, mods, #cracked, ninjastealth, leaks, ninja, stealth, leak, forums, modding, delusionalmods, delusional
Free forum : The world of anime has cracked and split up! Now once again all the dimensions have come together as one.
free, forum, anime, dimensions, world, #cracked, split, once, again, have, come, together
A 24/7 cracked minecraft server.
minestatus, publiccraft, 24/7, server, #cracked, minecraft
Free forum : #1 Minecraft Server! Several Worlds, Rp Prison, Factions, and PvP encouraged!
free, forum, citycraft, prison, minecraft, server!, factions, resources, creative, worlds, freebuild, towny, awesome, 2012, best, server, #cracked, diamonds
A Role Play forum that isn't generalized
#cracked, roof, role, play, isn't, generalized
Un forum pentru cei ce vor sa invete aceasta harta, cat si dorinta de a o face cunoscuta.
free, forum, fight, characters, pentru, invete, aceasta, harta, #dorinta, face, cunoscuta, focs, another, arena, hero, dota, garena, gplay, uijng, tistory


text:
I'm not engaging in hyperbole when I apply that descriptor to Ilana's death. Let's review:
- Jacob was the closest thing she had to a dad.
- She had trained her whole life for that mission.
- She knew tons about the Island.
- She had a big, unexplained backstory.
So why was she ignored all season, except "Dr. Linus"? And why did they kill her off like that?
Nothing about her is revealed. Nothing is resolved or changed by her death. It seems to have only happened for shock value-which is interesting, since most twists have something to do with the plot. This just happened for no f**king reason. And why didn't she give the dynamite to a candidate, or Richard? Neither can commit suicide, so presumably they can't be killed if they mishandle dynamite. As she was prepared for this mission by Jacob herself, you'd think that she would know this.
Did they want to establish how dangerous the dynamite is? Well, LOST FANS ALREADY KNOW THAT. From Arzt. Just namedropping him would accomplish the same function as her death. Having the dynamite go off and destroy the camp or something would accomplish the same function as her death. But no, they had to kill her off in the most meaningless way possible.
So to recap, they wasted a potentially major character on a cheap shock. In fact, Carlton hinted at it on Twitter by saying "tonight, a blast from the past". So...Ilana was killed as a homage to the first season!? I wouldn't be surprised if Damon and Carlton wanted us to laugh at it, or something.
And in earlier seasons, I might assume that her story would be told in flashbacks. But no. We don't get flashbacks, we get flash-sideways. Because, really, what more could you want in the final season than a series of recycled stories from earlier seasons with the twist that it's all about love (but only from "Happily Ever After" on, since they didn't set up the "they remember love" twist in any previous episode)?
As you can see on my user page, I will likely not watch the rest of season six, even if I'll hang around here still to edit stuff from earlier seasons.