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Sign up for the Automation Fair event newsletter. Get the latest news and information right to your inbox.
The forums at the 2016 Automation Fair® event featured examples and concepts that different manufacturers have used to increase their productivity and improve the efficiency of their business. These forums focused on how they:
2016 Session Materials Are Now Available for Download
Download the 2016 Automation Fair event industry forum materials using the Automation Fair event mobile app and desktop portal.
Automotive & Tire
The automotive and tire industries remain strong as manufacturers continue to invest in new technologies and plants. Increasing consolidation, industry regulations and consumer demand require new manufacturing approaches – across the supply chain – to remain profitable.
This forum focused on how industry peers are successfully implementing integrated control and information systems to improve their operations and thrive in a global economy.
The chemical industry is projected to grow through 2020 even outpacing the overall U.S. economy. Driven by strong demand and low inputs costs along with increased global competitiveness of US manufacturers, many companies are expanding operations and improving processes.
This forum featured industry experts discussing market outlook and trends and best practices from world class plants focused on plant-wide control and optimization to drive productivity, reduce costs, and drive efficiency in this rapidly expanding market.
Food & Beverage
Food and beverage manufacturers face a challenging environment driven by consumer demand requiring greater innovation and speed to market; while managing operating cost and efficiency. This is all compounded by greater regulatory pressures.
This forum discussed how leading companies are reaping the benefits of a Connected Enterprise and driving operational excellence and financial value in the food and beverage industries.
Global Machine & Equipment Builders (OEMs)
OEMs are challenged to continuously innovate and stay competitive. As more end users move to a Connected Enterprise to optimize their production and supply chain, they want added value beyond the machinery or equipment.
This forum featured how leading machine and equipment builders are capitalizing on this opportunity and are positioning themselves and their customers for greater success.
Life Sciences
Global life science companies face new challenges: innovation demands, speed to market, regulatory pressures and operational efficiency. Leaders in this industry are adopting new business models that deliver better patient outcomes at a lower cost.
The forum showcased how leading companies are using integrated information and control technology to meet their operational challenges.
Oil & Gas
Oil and gas companies strive for more efficient operations throughout their supply chains. Greater adoption of automation and information technology and associated services can help achieve operational excellence goals.
This forum provided an opportunity to hear how petroleum companies are using advanced automation and information technology to meet their operational challenges while maintaining focus on improving production efficiencies.
Power & Energy
From a "dash to gas" to regulation CWA 316B, the power and energy industries are dynamic. The effective use of technology will help improve both the effective generation and the efficient use of what's produced.
In this forum, leading companies and industry experts demonstrated how they manage these challenges and use information and automation to achieve a competitive advantage.
Pulp & Paper
The pulp and paper industry has been going through a significant transformation in the past few years. In a highly competitive global environment and limited skilled workforce, companies strive for more efficient and sustainable operations throughout their supply chains.
This forum provided an opportunity to hear how the companies are innovating and using new technologies to achieve operational excellence and environmental sustainability.
Water Wastewater
Globally, water treatment facilities are facing unprecedented concerns. For decades, the infrastructure has been able to handle and meet the demands of society; but today, aging infrastructure, growing population and urban sprawl are stretching treatment facilities beyond capacity.
In this forum, water industry professionals discussed industry trends and issues, the benefits of deploying a flexible and scalable solution, and methods to help reduce total life cycle cost and overall risk.


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Microsloth joke book
Q: How many Microsoft programmers does it take to write a great piece of software? A: More. You've read all the headlines: Microsoft Bids to Acquire Catholic Church and Windows 95 Ushers in Era of World Peace. You've bought all the software--even took out a second mortgage to buy it. You've installed all 45 disks of the latest Word program. And you've made 45 tech support phone calls to get it to work. Now what? Cry? Give up? Before you go Microsoft in the head, try laughing instead! The Microsloth Joke Book ...offers countless megabytes of Easy Install humor that storms the Gates and smashes the Windows of the world's biggest software company. Includes hard-copy files on * Windows 95 horror stories from Shakespeare to Captain Kirk * Bill Gates at the Pearly Gates * the first-ever Microsloth infomercial drinking game * the ten DOS commandments * songs * light bulb jokes * and much more. No Installation Required! No RAM Required! Sense of Humor Version 4.0 or Higher Required
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Scene Pictures
Start your 7 day trial at Cocky Boys here!
Lying in bed on a sunny morning in New York, Kody Stewart awakes next to Dillon Rossi and can't help but want to play with his massive cock. Dillon is still sleeping, but Kody takes his meat and starts sucking him to wake him up. Dillon indeed wakes up and doesn't mind at all and starts aggressively pushing Kody's head down on his cock. They start to 69 so that Kody can suck his dick while Dillon has a taste of Kody's hairy little hole, then they flip fuck in various positions.


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Spicy Shrimp In Coconut Milk
Recipe by MizzNezz
Steamed rice is perfect with this very tasty exotic dish.
Top Review by bydzign
This recipe is delicious. It reminds of a dish I had back home in NY at my favorite Indian restaurant -- Shrimp Malai. I've missed the dish and now can make it whenever I like! I've made it with seafood, chicken and will try lamb or veal stew meat. I serve it over Jasmine rice which a perfect accompaniment. This rice is light and fluffy and soaks up the sauce. I adapted the dish by adding extra tumeric and a little less than a tablespoon of sugar...yum :-). Thank you for submitting this wonderful recipe. I've already made five copies for friends who've tried it. Thanks! And keep them coming! :-). bydzign
See Discussion:95 Reviews 1 Tweak
Ingredients Nutrition
- 1⁄4 cup oil
- 1 cup chopped onion
- 1 tablespoon minced garlic
- 2 teaspoons minced fresh ginger
- 2 tablespoons coriander
- 1⁄4 teaspoon cumin
- 1⁄4 teaspoon cinnamon
- 1⁄8 teaspoon cayenne
- 1⁄8 teaspoon turmeric
- 1 cup drained canned tomato
- 2 1⁄2 cups unsweetened coconut milk (from 2 15oz cans)
- 1⁄2 cup water
- 1 teaspoon salt
- 1 1⁄2 lbs large shrimp, peeled and cleaned
- 3⁄4 cup fresh cilantro
- lime wedge (for serving)
Directions See How It's Made
- Heat oil in lg skillet on med-hi heat.
- Add onions and stir cook for 3 minutes.
- Add garlic and ginger,cook 2 minutes.
- Add next 5 spices, cook 1 minute.
- Add tomatoes,cook 1 minute.
- Add coconut milk,water, and salt, and bring to a simmer.
- Simmer until thickened 5-10 minutes.
- Add shrimp, simmer, stirring for 5 minutes.
- Stir in cilantro.
- Serve with lime wedges.


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Running Time: 95 minutes
Released: 01/2006
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Disclaimer: I don't own Alluro, Amok, Luna, or anything else pertaining to the Thundercats. Thundercats belongs to Ted Wolf and Warner Brothers. All I own is the plot to this story.
A Gentle Giant
He frowned at the great beast before him, moaning and groaning. The Lunataks had once again lost another battle to those meddling Thundercats and it was all thanks to the blunders of those miserable mutants. Alluro growled a bit, dipping the wash cloth into the bowl of hot water, ignoring the heat burning at his skin. He needed something to get his mind off of the looming failures lingering in his heart. After the battle, the Lunataks made a quick retreat back to Sky Tomb and Alluro soon found himself tending to not only his own wounds but to the ones of that big behemoth, Amok. Not that he actually minded, he just didn't like hearing that damned hag, Luna, barking out orders like a general giving orders to his army.
Just because she's the princess of the Moons of Plun-Darr doesn't mean we're here to serve her. The psychic Lunatak thought bitterly. Hearing the whimpers of Amok, Alluro quickly looked over at the pitiful creature. Its squinty eyes brimming with tears as it tried to lick at the stream of blood trailing from its thick hide under the leather shoulder pad. He wasn't going to lie. Alluro felt sorry for the poor creature.
Amok – despite his low intelligence and brute strength – actually had a strange gentleness that wasn't common for a Lunatak. Amok never really liked fighting. Hell, without Luna on his back, Amok was always free to enjoy the simpler things in life like those pretty flowers in the Enchanted Forests of the Unicorns or the chirping birds of the sky.
Alluro shook his head. He forced back the smile that was creeping onto his lips and he pulled out the soaked rag. The Troll-like Lunatak than went to Amok's side. He then started to dab at the wound. "That damned Panthro really did number on you, didn't he, Amok?" Alluro said softly, wiping away the crimson flow of liquid.
Amok merely whimpered, resisting the urge to swipe at Alluro, but he didn't. He sat there, giving Alluro a baleful look. "Amok hurt. Alluro help?" The creature grunted, its words making Alluro roll his eyes.
"Yes, you pitiful creature. Alluro help." Alluro bit his tongue to keep himself from yelling at Amok. He didn't want to hear Luna going into bitch mode at him mistreating her precious Amok, but in all seriousness, she should be tossed into the deepest pits of Fire Rock Mountain for all the times she caused Amok the emotional wounds with her sharp words.
Amok continued to sniffle and whimper, watching as Alluro now started to bandage his shoulder. His beady eyes notice a little scratch on the back of Alluro's hand. It was more like a bite mark actually and he knew just who gave it to him. "Snarf creature bite Alluro." Amok whimpered. His eyes shined with tears again.
Alluro just glanced at the bite marks on the back of his left hand and gave a shrug. "Snarf didn't do much damage." But it still hurts. He thought to himself. The Thundercats' pitiful nursemaid had lunged at him when Alluro tried to steal the Sword of Omens from Lion-O. I was so close in getting that damned sword, too!
Alluro was so caught up in his anger that he nearly jumped at feeling something brushing against his hand. When he looked down, he saw Amok licking at the specks of blood leaking from the wound Snarf inflicted earlier. Alluro couldn't help smiling at this gentle action from the creature next to him. Why couldn't Luna just allow Amok to be his true self – a gentle giant that deeply cared for others.
"Thank you, Amok." Alluro said softly, as the said creature licked up the last specks of blood.
Amok just merely grinned in that awkward way. "Alluro welcome." He grunted.
The two Lunataks looked up towards the ceiling at hearing footsteps shuffling upstairs above them and a loud voice came out of nowhere, screeching as it called for them.
"ALLURO! AMOK! WHERE ARE YOU TWO BLITHERING IDIOTS?" They can tell by the tone that it was Luna. Luna had woken up from her nap and she didn't seem to be in a good mood from the looks of things. Amok just whimpered in fright, his beady eyes turning back to Alluro.
"Just let her wait a little while longer. I still need to tend to your other shoulder." Alluro said, dipping the rag back into the hot water.
"But Luna angry." Amok protested.
"Yes, but do you want to be covered in blood?" Alluro asked, starting to get angry himself. He started for Amok's other side, beginning to dab at a small cut on the creature's flank. It never pays to be in the service of Princess Luna of the Lunataks.
Hina: I've had this idea for a long while but I just now started acting on it. I hope you've enjoyed this little weird one shot on Alluro and Amok. Other stories will be coming of course. Thank you for reading and reviewing.


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The TrackPoint Cap Collection is just the right touch for your ThinkPad notebook. Three TrackPoint cap choices allow you to further customize your ThinkPad to fit the way you want to work. You get two each of three different caps: Classic Dome, Soft Dome and Soft Rim.
Electronics, Computer & Photo > Accessories & Supplies > Accessories > Pointing Stick & Key Caps


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All Nail Problems Topics
- Sep 26, 2016
Are Jamberry Nails Safe for Your Nails?
The jury is still out on the question; are Jamberry Nails safe for your nails? Find out what others are saying and the best methods (such as flossing) on how to remove them. more »
- Aug 01, 2016
Is Black Streaks in Fingernails a Cause for Concern?
Black streaks in fingernails are usually caused by lack of nutrition, injuries, infections, etc. Here are more signs from your nails to predict your health condition. more »
- Jan 19, 2016
Why Are My Nails Splitting?
If you ask yourself why are my nails splitting, you are not alone. This common problem is due to dryness and other factors and can be easily resolved or rarely caused by diseases. more »
- Jun 02, 2015
Fingernail Ridges
Sometimes fingernail ridges are merely a product of genetics, but other times they can be signs of deeper underlying problems. It is important figure out how serious the problem is and then apply the best method for overall treatment. more »
- Apr 10, 2015
White Nail Beds
What causes white nail beds? What can nail color indicate? With the answers, you can check out your own conditions and know when it's time to bring it to a doctor's attention. more »
- Oct 10, 2014
Yellow Toenails
Yellow toenails are usually not potentially life threatening, but are cosmetically disfiguring and have potential to spread locally. Yellow toenails usually results from fungal infection of the nails. It is one of the most common disease conditions affecting the nails. more »
- Jun 27, 2014
White Spots on Toenails
White spots on toenails can be caused by various factors, ranging from dressing to nutrition to diseases. More information like treatments involving home remedies and medical treatments and preventions of white spots o toenails are displayed in the article as well. more »
- Feb 14, 2014
Peeling Nails
Exposing your nails to certain conditions or failing to provide the proper nutrients for your body can cause peeling nails. Understanding its causes can help you determine a proper solution and whether your condition requires medical attention. more »
- Sep 28, 2013
Thick Toenail
When nails become thick and yellow they can be difficult to manage. This condition is not only slightly, but a sign of possible health issues. Diagnosing the cause of thick toenails is essential to finding the appropriate treatments that return them to normal. more »
- Jun 26, 2013
White Spots on Nails
It is a common sign that people have white spots on nails. When determining if the spots on your nails are dangerous, it is important to make note of your other symptoms and how frequently the spots appear on nails. more »


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WASHINGTON — The National Retail Foundation on Tuesday said it was expecting the slowest holiday retail sales season since 2002 and said that gift cards could be among the items under pressure. In a conference call discussing its forecast for 2.2% overall sales gain for retail this season — well below the survey's 10-year average of 4.4% growth and the lowest since a 1.3% gain in 2002 — Scott Krugman, vice president of the NRF, said grim economic conditions and the likelihood of retailers dee
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Adelaide: New South Wales (NSW) was within two wickets of innings points to have South Australia on the rack in its Sheffield Shield match at Adelaide Oval on Saturday.
In reply to NSW's first innings 474 for six declared, South Australia was in deep trouble at 219 for eight after the second day, trailing by 255 on the first innings with two wickets on hand. A 93-run eighth-wicket stand between Shane Deitz and John Davison saved South Australia from being dismissed inside two sessions. Earlier, former Test opener Michael Slater built on his overnight score of 111 to top-score with 177 in the NSW first innings. He put on a sixth-wicket stand of 148 with Brad Haddin in opening session, before being bowled by medium-pacer Ben Johnson on the last ball before lunch, prompting the NSW declaration. Haddin finished unbeaten on 78, in just 98 balls, with 11 boundaries and a six. South Australia skipper Darren Lehmann did little to help his prospects of Test selection, being dropped twice before being caught in the gully off Nathan Bracken's bowling for 45.
Slater, Clarke slam centuries to put NSW in command


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- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Toys, Dolls, Games & Puzzles
- Source: eBay
- Sold Date: Dec 27, 2012
- Channel: Auction House
ebay STORE ABOUT US SAVE SELLER SUBSCRIBE TO NEWS CONTACT US3 VINTAGE FANTASY MINIATURES RULE BOOKS CHAINMAIL PHANTASTICAL EN GARDE GDW TSR
Description:3 vintage fantasy/miniature rules books. Books and their condition listed below. Included is: Chainmail - Rules for Medieval Miniature. TSR 3rd Edition 1975, shelf and use wear to the corners and edges, some dirt/staining on covers, previous owner's name stamped on page 1. The Phantastical Phantasmagorical Montie Haul Dungeon, 1st printing 1982, same condition as above. En Garde!, GDW revised/2nd edition 1977, same condition as above. We welcome local pick up at our store in Lakewood, CO. or use the shipping tab above to see service options and calculate costs. Shipping box: 0x0x0 About Us: Walk In Auction is a consignment service to help others sell their items on eBay - including individuals, businesses, and charities. We sell a variety of items and our inventory changes daily. We will always specify our opinion of the condition of an item and whether it was tested. We think we offer quality items at a great price and hope you agree! We try to give accurate descriptions and photos of the item so bidders can feel confident about their bid. Any item we sell is photographed just prior to listing and is always ... read more


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About the Book
Features & Details
- Category Poetry
Small Square, 7×7 in, 18×18 cm
38 Pages
- Publish Date Feb 06, 2009
About the Creator
The Freshman - Points of View is Courtney Robinson's debut as a professional poet but she is not new to writing poetry. In 1993 Courtney won the Maryland State NAACP ActSo division Poetry Contest with her poem 'I Don't want to be Alone'. Courtney went on to compete in the National Division and fell short but that did not discourage her! Courtney went on becoming the literary Arts Editor at FT. Meade Sr High from 1993-1995 and on to DuVal Senior High editor in 1996. Courtney took a ten year hiatus from writing and with the encouragement from family and friends began to write again. Courtney has begun sharing her work participating in the Artist and Author Showcase at Industrial Bank in Washington D.C. Courtney is currently finalizing her second book entitiled Venus which should be out at the end of the year.
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The Fantasy Sports Writers Association announced the winners of its 13th annual writing and media awards in a live broadcast on SiriusXM Fantasy Sports Radio on Tuesday, February 7. This year's winners were as follows:
Over 500 submissions were received across 22 categories this year. RotoWire led all companies with eight wins, while RotoExperts and Rotoworld also received multiple awards. Ryan Knaus of Rotoworld was a repeat winner in his category, Basketball Writer of the Year. Congratulations to all winners and finalists.


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MILLVILLE - What appeared to be marijuana plants were found Tuesday growing out of one of the many city-owned hanging flower baskets in the city's Glasstown Arts District.
The two plants, their leafy stalks sticking out above a patch of small pink flowers and black-and-white checkered flags, were spotted first by a passer-by.
The basket that held the plants was, fittingly, located on High Street.
No one else seemed to notice until the police arrived Tuesday afternoon and removed the plants.
According to onlookers at the scene, at least three uniformed officers propped up a ladder next to the lamp post and retrieved the basket early in the afternoon. The two plants were plucked from the bucket and taken to the police station, but not before a few photographs were snapped.
While police Capt. Tom Romanishin declined comment, saying the report had not reached him yet, the plants are easily identified by photos taken at the scene. The seasonal baskets, hanging from every lamp post on the street, look similar, aside from different colors of flowers. This particular one, located in front of a downtown eatery, was the only one featuring what appeared to be marijuana.
Just a few doors down in the Tri-County Independent Living Center, Mary Thomas and Pam Elliott joked about the discovery. Thomas showed off some pictures of the growth, taken with her cell phone, and had her theories about how the plants may have gotten there.
She said a police officer on the scene said marijuana seeds may have been thrown out of an apartment window above the street.
The flowers are maintained by the city's Parks and Recreation Department. Officials with the department could not be reached for comment.
Elliott wondered aloud how long the plants had been growing and why no one spotted them until Tuesday.
"I love flowers, right," she said. "We watch the guys every day, faithfully come out and water and fertilize every basket. That fertilizer is so good."
E-mail Edward Van Embden:


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Dance of the Geisha was a Silken Promises technique which taught the ability of a Silken Promise to entrance and fascinate a target borders upon the supernatural. [1]
- ↑ Vacant Throne, p. 53
|This Ronin related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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Kara Bumgarner, LMHC
Kara Bumgarner, LMHC
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More Info About My Practice
Accepts Multiplan, GHE (Sarasota County Schools EAP), Gulf Coast Provider Network (GCPN) and Gulf Coast Provider Network Select (GCPNS), Tricare, Value Options, and Private Pay. I do have a sliding scale and will bill insurances out of network as a courtesy.
My Guiding Ethical Principles
When I was in graduate school there were two ethical principles that really took root in my life. The first is "Do no harm." It is the job of a therapist to act as an advocate for the client. We must always put the physical and emotional safety of our clients above all else. We need to be sure to always act in the best interest of our clients. The other principle was "Therapist know thyself." It is imperative that the therapist is healthy and that therapy is always about the client and not ourselves. These two principles have been critical in the development of my philosophy along with the intention of always doing for others as I would want someone to do for me.
Services I Provide
- Individual Therapy & Counseling
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Ages I Work With
- Teens
- Adults
- Elders
- English
Groups I Work With
Individuals who are grieving especially those with long term illnesses and loss of loved ones, couples who are struggling with relationship issues including infidelity, separation, or divorce. I also help those struggling with anxiety, depression, trauma, and life transitions (including midlife issues, empty nest, aging, and caregiving for elder parents). I enjoy working with a wide variety of mental health challenges.
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- Abandonment
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text:
Editor's note: In January, The Insiders reported that Chalmers was receiving interest from Wisconsin. This week he cut his list to six schools and the Badgers were not among the favorites.
It's no coincidence that the AAU team North Carolina program.
"I'm not living my dream through my son," Ron said. "This is totally about him and my job is just to help guide him."
Mario is a 6-2, 175-pound Bartlett High (Alaska) junior floor leader who will take his first official visit this weekend to UCLA among his top six. The Tar Heels are the lone school yet to offer out of that group.
The old Matt Doherty regime showed Chalmers plenty of love, but Roy Williams and his staff really hadn't done much more than send the token letter until recently.
However, all that appears to have changed now that it looks as though JamesOn Curry will never end up donning Carolina blue.
"We want to keep our options open and we'd like to visit Carolina, the elder Chalmers added. "But we want to make sure wherever he goes, it's the right fit for him. We have to assess the situation and see where Mario fits in at each school."
Chalmers, who is hoping to make a college decision by November, has two more games before the playoffs begin. The team is 16-3 with all three of his losses coming against non-Alaska teams.
Chalmers averaged 10 points his freshman year and 15 his sophomore campaign, but he has begun to look for his scoring a lot more this season and it's translated into 28 points per contest.
Chalmers and his father will be the first ones to admit that the level of competition isn't really up to par. In fact, the family has been contemplating a move to North Carolina – where Chalmers still has plenty of family – for more than a year.
"I'd like to move so I can play against better competition," Mario said. "But we haven't made a decision on that yet."
Regardless of whether Chalmers leaves Alaska for his senior season, he'll play with the Pump 'N Run team this summer, which will be coached by his father. The first chance to get a look at Chalmers will be at the Kingwood Classic in Houston in late April.
"I've always liked North Carolina since I was little," Mario said. "But as far as front-runners goes, I really don't have one right now."


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text:
Genomic insights into an obligate epibiotic bacterial predator:
Although bacterial predators play important roles in the dynamics of natural microbial communities, little is known about the molecular mechanism of bacterial predation and the evolution of diverse predatory lifestyles.
We determined the complete genome sequence of
The findings from our phylogenomic and transcriptomic analyses shed new light on the biology and evolution of the epibiotic predatory lifestyle of
Predatory bacteria are a diverse group of bacteria that attack and feed on other bacteria. They live in various habitats and likely play an important role in microbial ecosystems
First isolated in 1983 from wastewater,
Results and Discussion
Genome summary
The complete genome of
Table 1
Main features of the genome of
Genome Size, bp
Predicted open reading frames (ORFs)
ORFs with assigned function
1228 (50.5%)
Conserved hypothetical ORF
193 (7.9%)
Unknown function ORF
124 (5.1%)
Hypothetical ORF
746 (30.6%)
Average ORF length, bp
Percent of genome that is coding
Ribosomal RNA operon
Transfer RNA
CRISPR element
Figure 1
Main features of the
Main features of the
Repetitive DNAs facilitate genome arrangement and increase the genome plasticity through homologous recombination. Strikingly, only 0.10% of the
Phylogeny and taxonomy
Figure 2
A maximum likelihood genome tree of alpha-proteobacterial representatives
A maximum likelihood genome tree of alpha-proteobacterial representatives. A maximum likelihood tree was built from concatenated protein sequences of 31 universal housekeeping genes and rooted by gamma- and beta-proteobacteria. Bootstrap support values (out of 100 runs) for branches of interest are shown beside them.
General metabolic features
Although an obligate predator depending on prey for cell replication,
Additional file 1
Comparison of major metabolic pathways between
Click here for file
Amino acid biosynthesis and transport
Our genome analysis suggested that
Among all bacterial and archaeal species sequenced to date, only a few species such as
Recently, it has been shown that bacteria can exchange cellular constituents (small molecules, proteins and DNAs) through intercellular nanotubes that connect neighboring cells, even between evolutionarily distant species
Hemolysin-related proteins
Table 2
Hemolysin-related proteins encoded by
Length (aa)
No. of Hemolysin-type calcium binding repeat
Other Motifs
Type I secretion system signal
GEIa in attachment/attack phase
Located within a genomic Island
Von Willebrand factor
aGEI: gene expression index
The hemolysin-related proteins encoded in the
Notably, hemolysin-related protein is one of few protein families that have been expanded in the
Secretion system and degradative hydrolytic enzymes
The genome of
Type II is responsible for the extracellular secretion of toxins and hydrolytic enzymes, many of which contribute to pathogenesis in both plants and animals. Proteins secreted through the type II system depend on the Sec or twin-arginine translocation (TAT) system for initial transport into the periplasm. The genome encodes a complete TAT secretion system (
Additional file 2
Hydrolytic Enzymes encoded by
Click here for file
Flagellum and pili
Additional file 3
Flagellum biosynthesis and chemotaxis genes of
Click here for file
Additional file 4
Gene expression index (GEI) derived from RNA-Seq. A excel file listing the gene expression index for all ORFs of
Click here for file
Signal transduction and quorum-sensing
Unlike other obligate parasitic bacteria such as
Lateral gene transfers
Additional file 5
Genomic islands in
Click here for file
Features found on these islands suggest that they belong to a group of integrative and conjugative elements (ICEs). Four out of nine islands are flanked by tRNA genes on one side and seven out of nine contain the signature integrase related to lambda phages (Additional file
ICEs allow bacteria to rapidly adapt to new environmental niches
Since ICEs can move between distantly related species by conjugation, it is natural to ask where did the ICEs in
Transcriptome analysis
To identify genes important in the predatory life cycle of
Approximately 72.6% of the genome (coding and non-coding) is covered by at least one read, suggesting that more than 27.4% of the genome was not transcribed or was transcribed at low levels in either phase. In addition, 91.6% of reads match predicted ORFs, indicating that there was very little background noise due to potential DNA contamination in our mRNA preparation. RNA-Seq has provided reliable quantitative estimates of gene expression in yeast and bacteria
Additional file 6
Correlation between qRT-PCR and RNA-Seq. An image file in PNG format showing the correlation between qRT-PCR and RNA-Seq data for selected genes in the
Click here for file
The transcriptome differs substantially between the attack and attachment phases. Overall, 80.0% of genes were transcribed in the attachment phase, but only 33.4% of genes were transcribed in the attack phase. Genes that were up-regulated in the attack phase are flagellar genes, chemotaxis genes and many hypothetical genes. Genes that were up-regulated in the attachment phase include housekeeping genes involved in DNA replication (e.g., chromosome replication initiation protein, DNA polymerase, DNA topoisomerase, helicase, gyrase), transcription (e.g., RNA polymerase, sigma 70, transcription terminator), translation (e.g., ribosomal proteins, translation initiation and elongation factors), energy production (e.g., TCA cycles, electron transport system, ATP synthase) and cell division (e.g., Fts proteins, cell shape determining factor MreB) (Additional file
Genes involved in protein secretion were also substantially up-regulated in the attachment phase. For example, our RNA-Seq data reveal a uniform increase of gene expression of the entire Sec secretion system (
Interestingly, three cold-shock protein genes (GMV0274, 1414, 2249) were highly expressed in the attachment phase but were not transcribed in the attack phase. Cold-shock proteins of
The most highly expressed gene (other than the rRNA genes) in the attachment phase is a porin-encoding gene GMV0043. Porins form aqueous channels on the outer membrane of Gram-negative bacterial cells, and control the diffusion of small metabolites like sugars, ions and amino acids across the outer membrane. GMV0043 was expressed at low level in the attack phase but was dramatically up-regulated in the attachment phase by more than 400-fold. The timing and intensity of the gene expression strongly argue that it plays a critical role in the attachment phase by facilitating the uptake of small metabolites derived from degrading prey cells. Similarly, Lambert et al. have showed that the maltose porin gene in
Strikingly, most of the highly expressed genes in the attack phase are hypothetical genes. This is in sharp contrast to the gene expression pattern of the attachment phase, where most of the highly expressed genes are well-known housekeeping genes. The fact that the hypothetical genes are highly expressed and the RNA-Seq reads match nicely to the gene models suggest that they are real genes. While uncharacterized, they most likely code for actual proteins that play cryptic but important functions in the unique lifestyle of
The phylogenomic and transcriptomic analyses of
Bacteria culture and genomic DNA preparation
Genome sequencing and annotation
The genome was sequenced by 3Kbp paired-end 454 pyrosequencing, in the University of Virginia Department of Biology Genome Core Facility, and was assembled using GS De Novo Assembler (Newbler). The initial Newbler assembly contained 21 contigs in one scaffold. The Phred/Phrap/Consed software package was used for quality assessment in genome assembly. PCR and Sanger sequencing was used to close the gaps between contigs to get the complete genome sequence, which was then annotated by the IGS annotation engine
Genome tree construction
Protein sequences of 31 housekeeping genes
RNA isolation, library construction, and transcriptome sequencing
To isolate RNA from attachment phase
Total RNA for both attachment and attack samples were isolated from bacteria pellet using RiboPure-Bacteria Kit (Ambion) according to the manufacturer's instructions, with genomic DNA removed using DNase I. RNA was quantified using Quant-iT™ RNA Assay Kit (Invitrogen). 23S and 16S rRNA were removed for mRNA enrichment using MICROBExpress Kit (Ambion). RNA quality analysis using Bioanalyzer (Agilent) indicated that about 90% rRNA was removed. cDNA libraries for Illumina sequencing were then constructed using NEBNext mRNA Sample Prep Master Mix Set 1 (New England Biolabs) following the manufacturer's protocol. Libraries were tagged, amplified by 15 cycles of PCR and sequenced with one lane of Illumina GA IIx 43 cycle single-end sequencing.
RNA-Seq reads mapping and visualization
Quantitative real-time PCR
Total RNA for attachment phase sample was reverse transcribed to cDNA using
List of abbreviations
ORF: open reading frame; BALOs:
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
ZW carried out laboratory work of genome and transcriptome sequencing and bioinformatic analysis and contributed to manuscript writing. DK prepared the cell cultures for genome and transcriptome sequencing and helped to draft the manuscript. MW conceived and designed the experiments, analyzed the data and wrote the paper. All authors read and approved the final manuscript.
We are grateful to Dr. Michelle Gwinn-Giglio for her help on annotating the genome sequence. Part of this study was supported by a grant to MW by the Thomas F. Jeffress and Kate Miller Jeffress Memorial Trust.
Bacterial Predators of
Prey bacteria shape the community structure of their predators
Phylogenetic Diversity and Evolution of Predatory Prokaryotes
Predation of human pathogens by the predatory bacteria
New species of exoparasitic bacteria of the genus Micavibrio infecting gram-positive bacteria
Vulnerability of pathogenic biofilms to
A new alpha-proteobacterial clade of Bdellovibrio-like predators: implications for the mitochondrial endosymbiotic theory
Analysis of the interpopulational interactions in a 2-component bacterial system of
Deciphering the hunting strategy of a bacterial wolfpack
Predatory lifestyle of
A predator unmasked: life cycle of
Evolution of sensory complexity recorded in a myxobacterial genome
CRISPR/Cas, the immune system of bacteria and archaea
Evolution of tuf genes: ancient duplication, differential loss and gene conversion
The genome sequence of
Genome sequence of the endocellular bacterial symbiont of aphids
Genome sequence of an obligate intracellular pathogen of humans:
Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation
Genome streamlining in a cosmopolitan oceanic bacterium
Complete genome sequence of "
Isolation and characterization of host-independent Bdellovibrios
Minimum nutritional requirements for growth of host-independent derivatives of
A New Method for Isolating Host-Independent Variants of
Genomic insight into the amino acid relations of the pea aphid,
The genome of
Intercellular nanotubes mediate bacterial communication
A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont
The interaction between RTX toxins and target cells
Role of iron in regulation of virulence genes
Lectins as cell recognition molecules
Role of carbohydrate receptors in the interaction of
Inhibition of Predation by
von Willebrand factor
Characterization of outer membrane protein fractions of Bdellovibrionales
Translocation of an outer membrane protein into prey cytoplasmic membranes by bdellovibrios
Genetics and phylogeny of RTX cytolysins
Biology of type II secretion
Isolation and some physicochemical properties of lytic proteinase of the parasitic bacterium
Development of a novel system for isolating genes involved in predator-prey interactions using host independent derivatives of
Type IV pili and twitching motility
Predation by
Characterization of type IV pili in the life cycle of the predator bacterium Bdellovibrio
Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow
Shaping bacterial genomes with integrative and conjugative elements
Pathogenicity islands: a molecular toolbox for bacterial virulence
Pathogenicity islands in bacterial pathogenesis
Deep RNA sequencing of
The transcriptional landscape of the yeast genome defined by RNA sequencing
Mapping the
CspA, the major cold-shock protein of
A family of cold shock proteins in
Activities of cold-shock domain proteins in translation control
Heat shock-induced axenic growth of
A predatory patchwork: membrane and surface structures of
IGS Annotation Engine
The Vmatch large scale sequence analysis software
A simple, fast, and accurate method of phylogenomic inference
A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood
FASTX-Toolkit: FASTQ/A short-reads pre-processing tools
Maq: Mapping and Assembly with Qualities
Artemis: sequence visualization and annotation
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method


text:
Ole Miss Theatre auditions are open to the university and local community. There are two auditions, one for the Fall Semester shows and one for the Spring Semester shows. Both are usually held at 1pm on the Saturday before the semester starts.
Fall Semester auditions will be held Saturday August 23rd and Sunday August 24th.
7:00pm – Second Callback for The Wedding Singer (Fulton Chapel)
To request information about our department and our programs, please submit an information request form


text:
Utah loss gives Lakers playoff spotApr 18, 2013 - 6:39 AM
Now, the Jazz will sit at home and watch the postseason on television for the second time in the last three years.
Zach Randolph had 25 points and 19 rebounds, and the Memphis Grizzlies beat Utah, 86-70, to eliminate the Jazz from playoff contention.
Utah's loss allowed the Los Angeles Lakers to clinch the final playoff spot in the Western Conference. The Jazz needed a win over the Grizzlies plus a Lakers loss at home against Houston to make it into the postseason. Instead, it's the Lakers going into the playoffs minus perennial All-Star Kobe Bryant, who will need 6-to-9 months to recover from a torn Achilles tendon in his left leg.
The Grizzlies will go into the playoffs as the No. 5 seed and will open on the road against the Pacific Division champion Los Angeles Clippers on Saturday. It will be a rematch of last year's first-round series, won by the Clippers in seven games.
"They've been a pain in our butts, but we've just got to go out there and worry about us, play hard, value every possession, play in and out basketball and stick to the things that got us to this point," Memphis guard Tony Allen said of the Clippers.
Mike Conley added 14 points for Memphis (56-26), which set a franchise record for most victories in a season in what will be its third straight trip to the playoffs. The Grizzlies also set a franchise mark for the most home wins in a season of 32, eclipsing the record of 31 set by the 2003-04 team.
Al Jefferson totaled 22 points and 16 boards for Utah (43-39), which had won nine of its previous 11 games going into the season finale. The Jazz shot 32.1 percent from the field.
"We are disappointed, of course, because we lost the game and didn't get a chance to make the postseason," Jefferson said.
Randolph did the bulk of his damage in the third quarter, when the Grizzlies widened their lead. He had 12 points and seven boards in the period.
Memphis' defense did its part too, holding the Jazz to 4-of-18 shooting in the quarter. Utah was just 9-of-35 from the field in the second half.
The Grizzlies entered the third with a two-point edge, but quickly found the game tied at 40 following a pair of Jefferson free throws. It was 50-47 midway through the third, when the Grizzlies took control by using a 15-2 surge that went almost to the end of the quarter. Randolph scored nine during the run.
It was 65-51 going to the fourth and the Jazz never got within single digits.
The game was tied at 17 after the first quarter and the Grizzlies held a 40-38 edge at halftime.
Game Notes
The Grizzlies agreed to terms with veteran forward Donte Greene on a multi- year contract prior to the game ... Greene didn't play this season after spending much of the campaign recovering from a fractured ankle he sustained during an offseason workout in August ... The Grizzlies have won six of their last eight regular season home finales after having lost the first 10 in team history ... Memphis has won at home over the Jazz in six of the last seven meetings ... Darrell Arthur scored 11 for Memphis.
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Related News
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- Russia bans 24 basketball players in gambling case May 13
- New Jazz uniforms include sleeved alternate jersey May 12
- Kings' methodical coaching search ended with Dave Joerger May 10
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text:
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18K Gold Fine Cross Pendant with Baguette Champagne Diamond
$ 900.00
Our 18K fine cross pendant features a delicate shape for a necklace that is understated and timeless. A baguette champagne diamond is the focal point of attention for this necklace. This recycled pendant is available in 16" and 18" lengths. The cross measures approximately 7/8" in length and 1/2" in width. The approximate total carat weight is 0.034. All diamonds are conflict-free.


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|Property type
|Minimum Stay
|4 nights
3 bedroom, 3 bath, 1-car garage with a 260 foot dock to the mile wide Indian River with picnic table at end of platform. 38' x 10' -350 square feet beach side porch- 50 feet from the Atlantic Ocean.
Fish the surf, the dock, or go wading in the Indian River Lagoon, or maybe some night fishing by dock light for guaranteed rewards of sea trout, snook, redfish, tarpon, grouper, and flounder to mention a few.
Wireless Internet available.
Washer and Dryer on the first floor, den amd utility room with full bathroom.
Kitchen equipped with oven, dishwasher, refrigerator, microwave, toaster, coffee pot, dishes, utensils, and gas grill.
Towels, Linens and beach chairs provided.
Sleeping accommodations:
Master - King size bed
Bedroom #2 - King size bed
Bedroom #3 - Bunk beds- full on bottom, twin on top, twin trundle
Two mile beach walk to St. Lucie Inlet, the gateway to Stuart, Florida. "THE SAILFISH CAPITAL of the WORLD."
Keywords: Private, Beach, House
I own the property along with my two brothers. There are too many of us to profile as I have 6 kids, my brother Jim has 5, and my brother Fran has 4. I chose my immediate family to profile.
Kathy Tight purchased this house in 1994
We never intended to use the house as a vacation rental. But we have enjoyed so many quality family vacations here, we wanted to share the experience with others.
The house is wide open and conducive to either a large group or quiet time for those just wanting to get away.
My family goes on yearly vacation, including my mom, dad, brother, his iwife and kids all join me and my family for a yearly vacation.
This year we decided to take our boats down to Stuart for some deep sea fishing. We towed my 26' Catamaran and my brothers 21' Carolina Skiff. The house had enough payrkng for 1 car, 2 full size pickup trucks, a 30' and 25' boat trailers. Everything you need is in the home, included the most beautiful sunrises and sunsets you have ever seen.
My dad fished off the dock all week and caught all kinds of fish including a very rare giant Mantus Shrimp!
All in all this was our favorite trip yet and we have made trips to Gatlingurg, Key Largo, San Francisco among others, this topped em all.
From the owner to the cleaning folks everyone was fantastic, we will be back soon!
We had a great time and the house is exactly as advertised. 8 of us stayed there for 4 nights and enjoyed sitting on the back deck watching the waves roll in. We also brought 2 boats and really appreciated the amount of parking (4 SUVs and 2 trailers). The dock is excellent too, although it is fairly high - making it a little awkward to climb in and out of our boats at low tide. Overall, I would give this house a 5 out of 5 rating!
I can say it truly was a great holiday. The 24hour non stop experience of the ocean sounding on the rear patio with sunset on the front patio was tranquil and incredible! The starboard side ramp walkway to the back door was so helpful for me with MS. It was an ease to load and unload in and out of the facility. The support team that helped me rescue a bad boating day was incredible. Thank You !! Hope you'll have me back there a lot more to explore in the Stuart Area and I can't wait to fish the inlet.
Capt Tom - USCG 100ton Master
Thomas G Henry - PRESIDENT CEO HENRY VENTURES INC.
The Beach/River house is positioned in an ideal location directly on the Atlantic Ocean with across the road frontage on the Indian River. Impressive to us was the rocky shoreline which is unusual in Florida, offering spectacular geyser like splashes from the crashing waves. On calm weather days, the water was close to Caribbean Blue, very clear, and breathtaking. The house is positioned close to the water's edge, which we LOVED. We sat in the covered ocean side porch rocking chairs for hours. Large sliding doors and screens opened the living area and master suite up to the porch so we never felt far from the wind and waves.
One feature of the rental that should be more emphasized is the handicapped access via a ramp (at times steep) from the parking area along the south side of the house to the ocean side porch and through the sliding doors to the second level of the home. The 3 bedrooms, 2 baths, kitchen and living areas as well as an outside porch overlooking the riverside allow handicapped guests free movement. This is unique as other rentals in the area require navigating steps to reach the second and/or third floors. The riverside ground level of the home provides a den and bath which is useful when accessing the river amenities. Brick pavers provide excellent access to the Indian River and wide wooden steps down to the water are helpful entrances into kayaks or other water toys. The long dock with a picnic table at the end would be paradise to fishermen and boaters who want to bring a boat and has water and electricity available at the dock's end.
Our party cooked most meals and found the kitchen to be very well stocked with sharp knives, cutting boards, Keurig coffee maker, blender, storage containers large and small, all types of cooking tools, pots, pans - probably the best equipped kitchen we've seen in our years of vacationing. The property was clean, well-maintained, comfortable, cozy and an easy place to relax. We did not require assistance from the owner. It was, however, the easiest to rent property we have ever encountered. Pay on VRBO and the owner emails the door code the week of your stay. Short, simple instructions are posted on the wall in the kitchen. Activities within walking distance are Bathtub Reef Beach and the House of Refuge. Just minutes away by car (less than 3 miles) along Hwy A1A is Elliott Museum, Publix, and a nature center for kids. We will return to this property - soon we hope!
My husband, his parents, our grown son and I had a wonderful time staying in your lovely vacation home on Hutchinson Island. What a delightful place to spend a week.
What a thrill to have the ocean with sunrise out the back, and the intercostal with sunsets out the front. We were steps away from both on this narrow section of the isle.
It is obvious you take great care and pride in keeping up the home. Your kitchen was marvelously stocked for every need. We did visit local breweries and restaurants, but spent most of the time eating in and enjoying the house and its views.
The furniture is very comfortable to sit in, and your dining room table and chairs are delightful with their beach colors. There is so much room, plenty for a much larger group.
Every one of us slept well, the beds are very comfortable.
Our parents (who are in their 80's) thoroughly enjoyed the front upstairs patio, where they could avoid the ocean breeze (which was too strong for them on some days while we visited).
My husband and son brought their own poles, and after purchasing fishing licenses at Walmart, loved catching fish off the dock. They caught enough that we had a Fish Fry for our Thanksgiving dinner!
The beach was wonderful to walk, and the unique stone formations along the shore were intriguing to investigate.
My husband, son and I spent a day kayaking at Jonathan Dickinson State Park, which is not too far south, and had a great time! We investigated Trapper Nelson's Interpretive Site 1/2 through our paddle as a way to stretch our legs, and was surprised to be greeted by a guide who gave us a tour of the entire complex.
My husband and I even went snorkeling down at Bathtub Beach at the end of the isle.
The only problem we encountered: the Eliot Museum decided to close early the day before Thanksgiving, so we did not get to go in. We ended up across the street at the Florida Oceanographic Society, which was amazing for seeing wildlife and learning about them. They let you get your hands on so many species. Due to some issues with the tank, we were not able to touch the stingrays upon arrival. We got a much better surprise later, as we got to feed the stingrays instead. This was a once in a lifetime experience, and the highlight of the trip for me!
Overall, if we are able to travel back to Florida, we certainly plan to stay at your home in the future.
We appreciate receiving the security deposit back in a timely manner, as well.
Our family had a wonderful vacation at your home! We couldn't have been more satisfied.. The beach is at your doorstep - the Indian River is at your other doorstep!! It was a wonderful time! Your home was very comfortable, and it was very easy to relax in.. All the conveniences - shopping, etc. - are within a 5-10 minute drive.. We are looking forward to our next vacation!
|Rate Period
|Weekend Night
|Monthly *
Feb 10 2017 - Aug 20 2017
4 night minimum stay
Aug 21 2017 - Dec 19 2017
4 night minimum stay
Jan 15 2018 - Aug 20 2018
4 night minimum stay
Nov 18 2018 - Nov 25 2018
4 night minimum stay
My Standard Rate
4 night minimum stay
|Cleaning Fee
|$150 + Tax
|Refundable damage deposit
|Tax Rate
* Approximate monthly rate. Actual rate will depend on the days of the month you stay.


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Sportsbook.com have prepared a list of odds for political aficionados with Democrat Hilary Clinton, a 6-1 favourite, running against Republican Rudy Giuliani, a 10-1 favourite.
With no incumbent president running, the race could produce some startling firsts. Sportsbook.com lists three African Americans as potential candidates. Barrack Obama and Colin Powell currently have odds of 30-1, while Condoleezza Rice is listed as a 15-1 favourite. What's more, two of the top four most likely candidates, Rice and Clinton, are female. This means that there is a very good chance the next presidential election could feature a female or visible minority presidential candidate for the first time in American history.
Adding to the fun and challenge, Sportsbook.com is offering odds on a number of less likely presidential candidates from film director Michael Moore to conservative news personality Bill O'Reilly and California Governor, Arnold Schwarzenegger.
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by Bill Holter, Miles Franklin:
I came across a couple of "monthly" charts last week and my face just lit up! The 2 charts above (courtesy of Trader Dan Norcini) are exactly why you can no longer wait to enter the metals in either "form." When I say either form I mean either through the mining shares (which are already 30%+ off of their lows) or the physical metal itself. When you look at these charts, please make very close note of the "MACD's." These are "the moving average convergence/divergence." Without getting too highly technical they are a "trading tool," let me explain.
Charts can be ultra-short term as in minutes or even seconds, or they can be very long term as in months or years. Obviously the very short term charts are used for trading while the long term charts will help you time your "big bets" for core capital.
Please follow SGT Report on Twitter & help share the message.


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Friday, August 7, 2009
Today Is My Birthday!
Hello, friends! I hope you have had a wonderful week!
Thank you again for taking the time out of your busy day to stop by my blog, and my guest posts, and leaving such sweet comments. I apologize for not being able to reply to your comments as I'm working on a huge project with a very tight schedule. It's fun, fast, intense, and will be complete by next Friday. I will be a little more absent until then...
I will be taking a little break from working tonight to go out for a birthday dinner with my family and boyfriend :)
/image link/
29 comments:
Happy Birthday Sarah! Love the balloon image. Please celebrate in style even though you are bery busy! I want pictures of the celebration!!!ReplyDelete
oh happy, happy birthday Sarah! we're having our birthdays very close! hope you have a wonderful time with your loved ones! :) xoReplyDelete
Hjärtliga gratulationer på Din födelsedag.. ....ReplyDelete
(happy birthday...in swedish).
Have I told you before that i LOVE your blog?
Regards from Sweden & Agneta
Nothing celebrates a birthday like a little Dior, so here's to hoping you get a little Dior! ;) Happy Birthday!ReplyDelete
Happy Birthday!!! I hope you have a great time tonight Sarah! Enjoy.ReplyDelete
Yay! It's your birthday! Celebrate! Have fun! Be spoiled!ReplyDelete
Eat chocolate...
Love you,
Auntie Jane
happy happy day, have a great dinner!ReplyDelete
Happy Birthday, Sarah!ReplyDelete
happy bday!ReplyDelete
Happy Birthday to You, Happy Birthday to You, Happy Birthday dear Sarah, Happy Birthday to You!!!! i wish you a super fabulous birthday celebration. i hope you have a lovely day!ReplyDelete
God Bless,
Happy Birthday! Hope you are having a fantastic day!ReplyDelete
Happy Birthday! My husbands birthday is today too, and as of today his newborn baby niece shares this birthday as well. Popular birthday date!ReplyDelete
Happy Birthday...ReplyDelete
you're the best daughter a Mom could ask for...big hugs and kisses
mmmm...where's dinner tonight...Sushi?
Happy birthday Sarah!ReplyDelete
happy happy birthday!!ReplyDelete
Happy Birthday to you!!ReplyDelete
Happy Birthday Sarah! I hope you feel like a princess for the day. What a sweet family you have commenting on your birthday post. Sounds like you have the 'best' of friends to celebrate with. x JulieReplyDelete
Aw, happy birthday! :)ReplyDelete
Girl, Happy Birthday to you!ReplyDelete
Have a wonderful day and a lovely weekend for of r'n'r...see you soon!
Happy Happy Birthday to you! Daisy~ReplyDelete
Birthday hugs to you!ReplyDelete
- AnonymousAugust 8, 2009 at 6:19 PM
Happy Birthday! I visited your blog for the first time today and am delighted and pleased.ReplyDelete
thank you. and good look on the project!
Love pictures like this. Fun, frivolous! Have a wonderful birthday celebration, and a successful project! Wendy xxReplyDelete
oh gosh sarah, happy birthday to you & many more wonderful years to come! goodluck on the project,too :)ReplyDelete
Hope you have a wonderful birthday.ReplyDelete
My birthday was lovely! Thank you all for your warm wishes - you made my day even more special!ReplyDelete
HAPPY BIRTHDAY!!!!!!!ReplyDelete
Happy, happy birthday darling! Hope you had the most wonderful day!ReplyDelete
happy belated birthday! hope it was lovely, lovely . . .ReplyDelete


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50 Cent is back with new music and decides to share the official remix to his single 'Im The Man'. He calls on the services of Chris Brown for the updated version of the Sonny Digital produced track.
Listen below.
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In today's Fix, we detail an interesting way to commemorate the first Xbox One ever to be sold, new updates on PS4 functionalities (One day to go!), and new updates coming to the 3DS and Wii U! Don't mind our new "studio look" our set will be back to normal tomorrow.
Here's what we covered:
The first Xbox One that will ever be sold is currently underwater. Don't believe us? See the image in the article linked above.
If you're getting a PlayStation 4, don't expect 3D Blu-Rays to be compatible. Will they be adding support in the future?
Why Sony changed their minds and their initial thoughts on these features.
In a new video Sony's own Shuhei Yoshida makes a bold statement about the two companies. Click the link for a looksee.
Nintendo details a new update coming to the 3DS. Here's what you'll get and when we predict it should arrive.
And to the Wii U! Here's when we'll get it.
Naomi Kyle is IGN's news host and on-camera personality. You can find her every day on The Daily Fix, kicking ass and taking names. You can follow her on Twitter @NaomiKyle.


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Read the full story here Web Link
Town Square
Sun skeleton
Original post made on Jun 20, 2007
Read the full story here Web Link
Comments (4)
a resident of Another Palo Alto neighborhood
on Jun 20, 2007 at 2:42 pm
In its time, this building was a great place to work. The April fools jokes that took place plus the newcomers orientation. Fond memories.
Once Sun left, it was allowed to go to rack and ruin and became an eyesore. As many of the happy memories I have from there, I am now definitely pleased to see it go. It should have happened long ago.
a resident of Another Palo Alto neighborhood
on Jun 21, 2007 at 10:20 pm
The Sun building site is the Bridge housing site only.
I wonder if toxic dust is being spread over the surrounding neighborhood's/? Has anyone called the county hazadard mtl. and are there air sample stations place around the site,neighborhood?
a resident of Meadow Park
on Jul 4, 2007 at 12:57 pm
I've written to the local air resources board, trying to find out what was released into the air when the SUN building was demolished. Also, how are neighbors notified about air quality? I haven't been able to find out anything yet. As work goes forward, is there a notification responsibility?
a resident of Another Palo Alto neighborhood
on Jul 4, 2007 at 5:22 pm
Same could be said for the 2 piles of fine debris at the end of Loma Verde and Bayshore. On windy days, you can see this dust fly off into the blue yonder. How long is it going to remain there undisturbed?
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Bay Roberts resident Keith Delaney learned early on the benefits off-ice training could have on his hockey career.
It started during his first couple of seasons with the Barrie Colts of the Ontario Hockey League. As a 17-year-old, Delaney was taught the ins-and-outs of keeping his body in shape over the summer months and into a new season.
That first year he posted 10 points in 64 games, but followed that up with 54 points in 66 games in his sophomore season with the Colts.
His dedication to training continued to evolve as he went through training camp with the NHL's Florida Panthers a year later after Delaney was taken in the sixth round of the 1997 entry draft.
Even when he was playing at the St. Mary's University of Atlantic University Sport, Delaney was making sure to keep off-ice training at the top of the list.
Now at 36-years-young, Delaney's continued to lean on the training he received over the years to keep him fresh for the grind that is senior hockey in this province.
"Off-ice training has always been a big part of my routine," he said. "It becomes a lifestyle. I take it as serious as I can."
When hockey ends in the spring, Delaney takes the first couple of weeks off each summer before re-dedicating himself to getting fit.
He's usually in the gym five or six days a week, although that might get a bit tougher with a pair of small children. It isn't unusual to see Delaney jogging along one of the roads in Bay Roberts during the summer.
This ferocious workout schedule has allowed him to stay at the top of the provincial senior hockey world with the Clarenville Caribous. As an elder statesman on a club that sits in second place in the four-team Central West Senior Hockey League, Delaney has been an integral part of the team's success.
Through 14 games, he has 11 points (five goals, six assists) and is eighth on the team in scoring.
"I think he's done a good job of avoiding a slide," said Caribous coach Dustin Russell. "Keith is playing very well and he's a good leader in the room."
Consistently great
Since teams moved to the provincial format ahead of the 2011-2012, Delaney has been at or near the top in league scoring every year.
Like that bottle of wine you put in the cellar years ago, it seems he only gets better with age.
Delaney's put up an average point total of 28 heading into this season. That impressive run starts with the CeeBee Stars when he posted 28 points. He followed that up with 27 points during the CeeBees Herder trophy run in 2013.
In his first season with the Herd, Delaney posted 26 points and followed that up with a 34-point campaign in 2014-15. He finished fourth in league scoring last year.
"I thought last year was possibly my best," he said. "It's a challenge every weekend."
Delaney credits his linemates - younger brother Ryan Delaney and Ryan Desrosiers - for a lot of his success on the ice.
"We've developed some good instincts with each other on the ice," he said. "Ryan and I have been together forever and Desrosiers fits in nicely. I've had the same linemates for several years. Since I got here, it's been the same three of us."
A do-everything player
Delaney wears many hats for the Caribous during a game. He plays a regular shift when its five-on-five, along with killing penalties and playing on the power-play.
"We use Keith in all situations," said Russell. "He takes a regular shift and he has been playing very well. He's one of our assistant captains. Keith is right up there and is in good shape.
"He's very dedicated. He definitely pulls his own weight."
As he is set to turn 37 in May, the question inevitably becomes how much does Delaney have in the tank.
Delaney doesn't have the answer. The only thing he is sure is his desire to play the next couple of years.
"I'd like to be around hockey as long as I can," said Delaney. "I'd like to get another championship."