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2-7-16 – "No Eye Has Seen . . . No Ear Has Heard . . . "
Consider the times…
1 Corinthians 2:8-16 –
"the wisdom which none of the rulers of this age has understood;
for if they had understood it
they would not have crucified the Lord of glory;
but just as it is written,
"Things which eye has not seen and ear has not heard,
And which have not entered the heart of man,
All that God has prepared for those who love Him."
For to us God revealed them through the Spirit;
for the Spirit searches all things, even the depths of God.
For who among men knows the thoughts of a man
except the spirit of the man which is in him?
Even so the thoughts of God no one knows except the Spirit of God.
Now we have received, not the spirit of the world,
but the Spirit who is from God,
so that we may know the things freely given to us by…
View original post 770 more words
Bodywork for the Summer Enthusiast
Summer is a wonderful time of the year. I am guilty of holding off, waiting to complete some tasks and activities till the sunny days of summer. Now is the time of year we fill with outdoor activities: running, biking, softball, golfing, tennis, water sports – you name it, and it's going on somewhere in the region! We don't hold back, but enjoy every minute we can!
As we give it our all, we also feel the aches and pains of muscles that haven't been challenged at this level and in this manner for some time.If you are an avid bicyclist, during your outing, your quads will be used in demanding, short extension durations, your neck will be arched for safely scanning your environment while biking, your arms and shoulders will have demands from the forward positioning of your body; your back, on down your hips and glutes, are working in very specific ways unique to bicycling.
Contrast that to a tennis enthusiast. The same muscles are firing, yet in completely different ways. The quads, calves, and knees are challenged in different twists and turns and abrupt start and stop patterns. Your neck and shoulders are posturing different for meeting the trajectory of the tennis ball and strategizing your return shot. Your arms and elbows are experiencing a completely different impact to meet and return your shot. Your back and hips are responding with a greater range of motion and holding pattern to return the volley and capitalize on your opponent's play.
In both sports, many of the same muscles are used to complete their unique challenge. The interesting aspect is although the muscles are firing, each are being utilized in different degrees, ranges and durations.A massage session can relieve the aches and pains from overdoing your favorite sport or hobby and give you some down time for renewal.
Your session at élan is customized to your specific needs. When you arrive for your session, you will be greeted via a soft, low-key transition away from the hustle and bustle of everyday life. You'll encounter soft lighting, gentle ambient music, light aromatherapy (if acceptable to you), a glass of water to continue your hydrating, and most importantly, time to relax and share your needs and goals for today's session. I will take time to ask questions relevant to our work today, and together determine a plan to help you renew and return to balance.
You are unique; your needs are recognized and treated in a caring, intentional, intuitive manner.
Your session will begin with quiet connection, breathwork, and visualization as we invite the body to return to a state of balance. I will never "force" a technique, but always lead with a soft introduction to the muscle or tissue being worked on. Does this mean there is no deep tissue work? There IS deep tissue work, if needed, but all work and techniques are done in a caring, intentional, intuitive manner.
Especially if this is your first time at élan, I will quietly add insight to what is occurring, so the body-mind connection becomes a part of you. Learning to listen to your own body is an asset that will serve you well your entire life! Clients who have come on a regular basis have a deeper understanding of what their body is telling them and can make decisions in their best interests.
A client was in earlier this week, aware of the tightening in one side of her jaw and the tension in the back of her scalp and on down her left shoulder. She connected this to specific stressors occurring in her life at this time. Observing and acknowledging what is transpiring is a wonderful attribute that helps in every situation in life. It's one more facet of living in the NOW. I feel privileged to see this in action in my clients' lives.
I invite you to take some time for your well-being this busy time of the year. There's no time like the present to become the best you there can be!
Sue is an experienced, intuitive bodywork (massage) and energy healer dedicated to coming along side you on your journey. You instinctively know when it's time for a change, whether working with aches and pain in the physical body or resolving imbalances or discomfort in your energy BE-ing.
If you are hurting and ready to begin a new chapter in your well-being, call Sue at 605.254.3665. See more at http://www.elanhealingarts.com
Sue Jacobs
élan Healing Arts
What Should I Expect During a Massage Session?
What should I expect during a session?
If this is the first time you've contemplated a massage or if you received a gift certificate, you may have questions on what to expect. I hope the following clears up any "mystery" or nervousness about the session!
What's the difference between massage and bodywork anyway?
The terms are very similar. I tend to consider more of your session bodywork, as a broader range of techniques are used: massage, gentle stretches, gentle range of motion, gentle rocking, reflexology, guasha with a jade stone, tennis balls to relax areas, trigger point therapy and intuitive touch may be used.
Where will my session take place?
My studio is in a quiet residential area, away from the hustle and bustle.
Your session will take place in a warm, comfortable, quiet room. Soothing, ambient music and soft, dimmed lighting will welcome you to help you relax.
How will the therapist know what to do for me?
If this is your first time at élan, I will ask you to complete an intake form where you can identify your applicable medical history, medications, current concerns, and your objectives. Before your session, we will take time to discuss your needs and answer any questions that may arise.
Do I have to completely undress?
Most massage and bodywork techniques are traditionally performed with the client unclothed (under a sheet and blanket). For a full body session, I encourage you to disrobe to your level of comfort; a female is encouraged to also remove her bra, so long strokes and techniques can be completed on her back for the best results. Removing panties/briefs is optional. If left on, and work is done on your lower back/hip area, the top border of your underwear will be tucked down slightly with the sheet, always maintaining your privacy.
Will the therapist be present when I disrobe?
I will leave the room while you take a sip of fresh water, undress, relax onto the warmed bed, and cover yourself with the clean sheet and blanket. I will knock and await your response before opening the door and returning to begin your session.
Will I be covered during the session?
You will be properly draped (with a sheet and blanket) during the entire session to keep you warm and comfortable. As a legitimate, professional massage therapist, I assure you, your genitals or breasts, and any other areas you identify, will remain covered. Only the area worked on will be exposed, then re-covered.
What parts of my body will be massaged?
A typical full-body session will include work on your back and hips, neck and shoulders, arms and hands, legs and feet, and head, including face, scalp, and occipital.
What will the bodywork feel like?
We begin your session with breathwork and visualization to encourage relaxation for the body, mind, and spirit. Relaxing Swedish massage is often the beginning technique, with broad, flowing strokes that help calm your nervous system and relax muscle tension. As your body becomes more relaxed, and based on your needs expressed in the pre-session interview, pressure and techniques will be incorporated to relax specific areas, relieve areas of muscular tension, and recalibrate/ balance areas of imbalance .
A light oil or lotion is used to allow your muscles to be massaged without causing excessive friction to the skin. The oil also helps hydrate your skin. Whatever is placed ON your skin is absorbed INTO the skin, so quality, organic lotions are used.
Let me know right away if you feel any discomfort so that another approach may be taken. Bodywork techniques are most effective when your body is not resisting. Our focus is on balance, allowing, inviting the muscles to relax and recalibrate.
What should I do during the session?
Prior to the massage, feel free to ask me any questions about techniques or the upcoming session. During the session, make yourself comfortable. As we proceed through the session, I will either gently move you or tell you what is needed (such as lifting your arm). Many people just close their eyes, focus on their breath, and completely relax. You are always welcome to communicate if/when you need more or less pressure, another blanket, or anything else relevant to the session. If you have any questions regarding the session or about the particular technique you are receiving, feel free to ask.
How will I feel after the session?
Most people feel calmer, relaxed, more open. Some experience freedom from long-term aches and pains developed from tension or repetitive activity. After an initial period of feeling slowed down, people often experience increased energy, heightened awareness, enhanced body-mind connection, and greater productivity that can last for days.
What are the benefits of bodywork?
Bodywork can help release chronic muscular tension and pain, improve circulation, increase joint flexibility, reduce mental and physical fatigue and stress, promote faster healing of injured muscular tissue, improve posture, and reduce blood pressure. Bodywork may also promote better sleep, improve concentration, reduce anxiety and create an overall sense of balance and well-being.
Relax… this is YOUR time
Are there any medical conditions that would make bodywork inadvisable?
Yes. That's why it's imperative that, before we begin your session, you provide your health background via the intake form, and subsequently, with updates to your health before each session. It is very important that you inform me of any health problems or medications you are taking. If you are under a doctor's care, it is advised that you receive a "green light" for massage or bodywork prior to any session. Depending on the condition, approval from your doctor may be required.
In a future post, I will discuss what makes élan different than the chain or spa settings.
Tune in, there's more to come for your well-being!
I look forward to talking with you about bodywork and what it may do for you! Call soon to schedule your session!
For more information, visit: http://www.elanhealingarts.com/faqs.html
Sue Jacobs, élan Healing Arts ∞
Phone 605.254.3665
Image courtesy of Ambro / FreeDigitalPhotos.net
Earthing – Not Just For Summer!
What can Earthing do for you?
- Look better
- Feel better
- Sleep better
Earthing connects us back to the earth's natural energy. Check out http://www.earthing.com/
When we use earthing products, our bodies are re-connected to the earth's natural energy. This in turn can re-energize, re-balance, and re-new our beings. I began with the Earthing Starter Kit
In our locality, this winter has turned into an endurance race. The ice storm experienced by West River (South Dakota, USA) in October 2013 began the season, and there's been nary a warm day, or even average day since then. Weather predictions continue with below average temps and wind chills for much of March as well. Needless to say, this is not conducive for sitting outside in the sunshine, with bare feet. Even the Polar Plunges are quickly completed and the participants re-covered and protected from the severe cold!
I've been able to thoroughly appreciate my earthing bedding and mat during this long indoor season. These are improving my meditation time and quality of sleep. Sleeping in the earthing bedding may be reducing inflammation in joints (hubby's testimony), and helping us cope with the stressors of compromised indoor air quality and seasonal affective disorder.
If you don't feel well, for whatever reason, just make barefoot contact with the Earth for a few minutes and see what happens. Of course, if you have a medical problem, you should see a doctor. There is nothing that comes close to Earthing for quick relief. You can literally feel pain draining from your body the instant you touch the Earth
If you have questions or wish to discuss, please call – I'd love to discuss the benefits for YOUR well-being!
photo credit: Lone Primate via photopin cc</a
The Virtues of Epsom Salts
The Virtues of Epsom Salts
With the change of seasons, it is the time of year for shifting gears into colder weather and holiday mode. A wonderful treat to help your body and mind adjust to winter and all the demands placed upon you is a good old-fashioned Epsom salt soak. Your grandmother was right – an Epsom salt soak is sometimes exactly what is needed!
Epsom salts are magnesium at its finest, magnesium salts. Interestingly, magnesium is one of the first minerals to deplete when food is processed (rather than real). It is also one of the first to leave the body when stress is experienced. (Have you had any stress in your life lately?)
Whatever is placed on the skin (or soaked in this case), is absorbed into the skin (considered transdermal). When you place healthy ingredients on your skin, you are assisting your body to relax and recalibrate.
Our bodies are so amazingly designed!
Magnesium is one of the essential minerals for your body's chemistry. This can help with sore, fatigued, cramping muscles, stress and fatigue. The main symptoms of magnesium deficiency are insomnia, muscle cramps, anxiety, constipation and heart palpitations.
Cortisol is a stress hormone – the "fight or flight" hormone. Magnesium can be considered an anti-stress buffer, because it decreases the cortisol, aids muscles to relax after the muscle firing. For the sleep-deprived (that is, MOST of us!), it can prevent night time muscle cramps (Charley-horses) and stress-related sleep issues.
A safe, welcoming way to up your magnesium level is through the use of Epsom Salt (magnesium sulfate) Baths.
- Start with half a cup (gradually increase up to two cups) of Epsom Salt in a hot bath.
- Soak – Relax – Unwind
- Repeat tomorrow if necessary!
Isn't it time for some YOU time?
For more info on Epsom salts or if you would like delivery right to your door click this handy link!
Dr. Teal's Epsom Salt Soaking Solution, Soften & Nourish with Milk and Honey, 48oz
Image courtesy of winnond at FreeDigitalPhotos.net
Source: http://www.saltworks.us/salt_info/epsom-uses-benefits.asp
Illusion of Connectedness
A couple weeks ago, our area had a massive ice storm. As the rain and sleet were falling, the ice kept building up. As the day progressed, the weighted down trees groaned under the stress. The sound of massive limbs snapping off our beautiful trees randomly echoed throughout the neighborhoods.
The trees look spindly now, off kilter, with the fullness of the tree limbs missing. The clean up efforts have continued, with large piles of jagged big branches and stumps in the boulevards.
The amazing thing to me as I walk through the neighborhoods, is the limbs removed have actually started budding, ready to green out. The communication hasn't been fully received yet at the budding ends, that the source is gone.
This made me reflect on my journey. Am I on auto-pilot through the day? Am I focused on only what's in front of me? Am I self-absorbed, reacting to the moment by moment tasks?
I then realize without remaining connected to Source, before long, my efforts are futile.
To move with intention, to live with purpose, I cannot do life alone. I need to ground, I need to return to Source.
What does grounding mean to you? Who is your source? Connection with community and with something, someone bigger than yourself is so important!
Consider an energy session / quantum touch session to increase your awareness of the present.
I look forward to working with you!
Is It Time for Your Spring Cleanse?
Spring has sprung! Soon the white backdrop of snow will completely disappear and be replaced with the beauty of fresh tufts of green grass, trees budding, and bright spring flowers.
What does spring cleaning mean to you? Opening windows to air out the house? Mopping floors, shampooing carpets and washing windows? Packing away winter clothes? Our homes feel lighter, brighter and cleaner after the process.
How about considering a similar cleansing and rejuvenation for your body? Now is a wonderful time to release what is no longer needed and clean out winter's sludge. Often restful and revitalizing, cleansing and detoxing also help us create healthier lifestyle habits.
With the season change, it's a wonderful time for your spring cleanse.
Review your wardrobe. Did any items in your wardrobe "shrink" since last season? What items can be released (shared, paid forward, or discarded)?
Review your food plan. Are you feeling sluggish? Is it time to recharge your body with quality fuel (healthy live foods)? The palate of seasonal foods is fresh and colorful once again.
Rethink your temple upkeep. A spring cleanse will release the waste and buildup from winter eating habits along with preparing the body, mind, and spirit for the fresh, new beginnings of the season.
Three Things to Consider for Your Spring Cleanse
Massage: a full body massage can help move the toxins out of the body, lengthen and relax muscles, release the knots and tense areas. A full 75 minute session dedicated to your well-being can bring the cortisol levels down, relax tense muscles, renew spent energy, and re-calibrate your inner balance.
Dry Brushing: This session utilizes a dry, natural bristle brush to stroke your skin, following the lymph drainage system. This moves toxins and waste out of your body and has the added benefit of exfoliating your skin, brushing away the dry, flaky skin, so the fresh, radiant skin can glow with the fresh air and sunshine.
Sauna: after your massage or dry brush session, enjoy a private session in the dry, infrared sauna. As the heat rises, your body's temp mechanism is activated to maintain its balance. As a result, toxins are released, fresh nutrients are brought to the body's surface, and the vasodilation relaxes the vessels, which can lower pain. The quiet, relaxing time in the sauna is a respite from the busyness of your day. As the infrared heat does its work, your body and mind can relax, focus inward, enjoy a time of quiet reflection.
Spring Cleanse Package Specials!
Select two of the three options of *Massage*, *Dry Brushing*, or *Infrared Dry Sauna* for your Spring Cleanse Value Package! $100
Call 605.254.3665 today to reserve your session – or pamper your loved one!
You Are SO Worth It!
In Gratitude and Appreciation
Being a member of this "human race" is such a privilege! Even though we all share this space, we each have different outlooks, filters, and gifts. I feel so grateful that I can wake up, get up, and work with clients who need help with pain issues, stress, or just recognize their need for personal time.
In dialog with clients and colleagues alike, I continue to be amazed at what drives each of us, what makes us tick. I am so grateful for the set of gifts and talents I recognize in you and am able to connect with. And as time progresses, I see the skillset and passions develop into more powerful attributes as contributions are made in the day by day landscape. Colleagues with savvy presentation and design skills, artists who know how to capture the moment in photography or paint or word or music, orchestrators who can take group input and bring a project together in a multilayer, cohesive frame of reference.
I am in awe when I reflect on the people who have shared today's journey with me. Why do I share this?
Because the beauty of life and those around me bring me to an even greater appreciation for the gifts and passions that unfold each and every day.
I see in the human body and its integration only one multilayered aspect of a person's being. I see the delicate layers of a friends' passion and drive and realize my work complements their strengths. No one part is greater. No one area is lesser. Each has its part in that person as a whole being.
You have a unique, made just-for-you set of talents. Listen close, choose your own life's path and quality relationships that you want to foster and cultivate! You truly have access to a vast inner power; you can move past anything that is not congruent with your beliefs and passion. You are unique and you are the best you there is!
Vivo bene!
(Live well)
photo credit: <a href="http://www.flickr.com/photos/vickiehallmark/5372723363/">VickieHallmark</a> via <a href="http://photopin.com">photopin</a> <a href="http://creativecommons.org/licenses/by-nc-nd/2.0/">cc</a>
The Power of Connection
Ah, the Power of Connection
Oft times, even the mention of "connection" sounds "fluffy" and easy to dismiss. Yes, we all need connection, it's just out of sight, off the radar most of the time.As we launch into the holiday season, it will be to our benefit to get our feet firmly planted, breathe calmly, and focus clearly. If we do not prepare ourselves beforehand, we just may get swept away with the stress, the frenzy and the hype.
Before leaving home, plan out your game plan. Keep reminding yourself what all this activity really means to you and your loved ones.
Consider: maybe the best present is to be present, to be in the moment, enjoying the time, laughter, and joy of relationship.
Maybe the best present is to be present
If that sounds too "It's a Wonderful Life", then bring it down a notch, into something more plausible, more relevant to your life.
Maybe it's just limiting the gifts, and expanding the time and interactions. Maybe it's having a quiet evening of soup and conversation, then going out together to view Christmas lighting in the area. Maybe it's softening the lighting, listening to holiday music or watching your favorite holiday flick.
In the bigger picture, it's the memories, the laughter, the connectedness that is remembered.
"The ability to be in the present moment is a major component of mental wellness." ~ Abraham Maslow
May the weeks ahead bring you joy, laughter, centeredness, connectedness. May you enjoy the present of presence!
photo credit: <a href="http://www.flickr.com/photos/laszlo-photo/4191193825/">laszlo-photo</a> via <a href="http://photopin.com">photopin</a> <a href="http://creativecommons.org/licenses/by/2.0/">cc</a>


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Interracial House Of PussyFavorites [+]
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Running Time: 118 minutes
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Studio Name: Damaged Productions
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Bebisol pacifier Reversible Silicone Rose Ptite dreamer + 6 months (ref No. 4)
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Wonder Woman - off to work by stevelydicWatch
©2011-2017 stevelydic
just finished this piece with a combo of prismacolor and copic markers.
i really love how natural she looks it really what makes it art instead of pointless fan service
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ah that a cute concept. Also like the inking of the drawing came out very clean and the coloring looks nice ^_^
a very sexy WW pic... nicely done!
thanks for the comments!! i tried to stay on the cute end with this and stay tasteful.
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Came out great


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Dual Monitor Software to Control Dual Display Devices or Monitors
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Today, at work, a customer came in and ordered a "Butterbeer Frappuccino." When I said we serve no such thing, she yelled at me for "lying" to her, saying she knew about our "secret menu." She ended up complaining to my manager and demanded that he fire me. FML
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Ready for the premiere of Adam Mason's Pig this weekend? Let's hope you have the stomach for it! To get your prepped (as if anything actually could), we have gotten our hands on four brand spanking new lobby cards for the flick!
Pig (review here) will simultaneously have its world premiere right here on Dread Central and over on Bloody Disgusting and Twitch. Pretty nifty, huh? All you have to do is show up, tune in, and dig it!
Just be here on Saturday, April 17th, at 6pm PDT/9pm EDT, and we'll do the rest! Look for more details soon!
Twitch film describes Pig as:
"Together with frequent collaborator Andrew Howard, Mason has completed an entirely different film during the waiting period for Luster. Shot on the cheap and on the sly, with any word kept carefully out of the press until the film was complete, Pig is a grueling exercise in single-take horror, a savage satire on American gender roles built around a lunatic central performance from Andrew Howard. With the runtime dominated by a single 70+-minute take, this is something entirely new from Mason, a technical experiment that builds on the themes and approach of his earlier work."
Click on the images below to see them bigger, and for a few others check out Bloody Disgusting!
Got news? Click here to submit it!
Squeal for help in the Dread Central forums!


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It's time for the next installment of "Happier with Gretchen Rubin."
Update: For our upcoming Very Special Episode, Holiday edition, we want to hear from you: What is your Try This at Home for staying happier, healthier, and more productive over the holidays? It can be a challenge. So let us know what works for you — for dealing with family, for traveling, for managing temptations, anything. We can all learn from each other.
Today is the fourth in the series of four episodes that we're devoting to the Four Tendencies. In last week's episode, we talked about the Obliger Tendency; this week, it's Rebel.
To find out if you're an Upholder, Questioner, Obliger, or Rebel,
take the Four Tendencies quiz here.
Try This at Home: Try to come up with a motto for your Tendency. Fun!
Strengths and Weaknesses of Rebels: How to identify and take advantage of the strengths, and counter-balance the weaknesses, of the Tendency.
Striking Pattern of Rebels: Whenever a Rebel is in a long-term relationship, whether romantically or at work, it's almost always with an Obliger.
Another striking pattern: While Rebels want choice and freedom, some Rebels are drawn to areas of high regulation, such as the military, the police, and the clergy.
Listener Questions: "What are some strategies to use if you have a Rebel child?" "How do Rebels manage their inclination to rebel against themselves?" Plus a Rebel weighs in about how she sticks to her good habits.
The Rebel author I mention is Geoff Dyer. I highly recommend his book Out of Sheer Rage: Wrestling with D. H. Lawrence as a self-portrait of a Rebel.
Elizabeth's Demerit: Elizabeth needs to get her car serviced.
Gretchen's Gold Star: I've started a new habit: on my Facebook Page, each Sunday evening, I post a photo of all the books I've read that week. I love to shine a spotlight on books, and I get a lot of satisfaction from thinking, "Look at what I've read."
Call for comments, questions, observations!
We've spent four weeks talking about my Four Tendencies framework for human nature. It has been fascinating. We've had so many terrific responses that we're planning a round-up episode. So if you have more questions or comments, send them in!
- Take the Quiz to find out if you're a Upholder, Questioner, Obliger, and Rebel here.
- Listen to Elizabeth and me discuss the framework here.
- Read the chapter on the Four Tendencies in Better Than Before
- Please, send in your questions and comments by voicemail, email, etc.
As always, thanks to our terrific sponsors
Check out The Great Courses for a wide variety of fascinating courses. Special offer for our listeners: go to thegreatcourses.com/happier to order from eight of their bestselling courses, including The Everyday Gourmet, and get up to 80% off. Limited time.
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Tell us — did you come up with a motto for your Tendency?
If you're intrigued by the Four Tendencies, and want to be notified when my handbook on the subject hits the shelves, text me at 66866 and enter the word "tendencies," I'll add you to a list to be notified when it's ready. You can also sign up here.
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To sign up for my free monthly newsletter, text me at 66866 and enter the word (surprise) "happier." Or click here.
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Want to know what to expect from other episodes of the podcast, when you listen to "Happier with Gretchen Rubin?" We talk about how to build happier habits into everyday life, as we draw from cutting-edge science, ancient wisdom, lessons from pop culture—and our own experiences (and mistakes). We're sisters, so we don't let each other get away with much!
HAPPIER listening!


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Get an overview of this hotelHelsinki hotel in Kamppi, near Kiasma Museum of Contemporary Art
Popular property highlights
Located in Kamppi, this hotel is within a 15-minute walk of Sinebrychoff Art Museum, Kiasma Museum of Contemporary Art, and Ateneum Art Museum. National Museum and Finlandia Hall are also within 1 mi (2 km).
Hotel Features
This smoke-free hotel features laundry facilities, express check-in, and express check-out. WiFi in public areas is free.
Room Amenities
All 95 rooms provide conveniences like refrigerators and microwaves, plus free WiFi and flat-screen TVs. Premium bedding, coffee makers, and hair dryers are among the other amenities available to guests. Weekly housekeeping is available.Information missing or incorrect? Tell us!Opens in a new window
Forenom Aparthotel Helsinki City
Hotel Amenities
Hotel Amenities
Forenom Aparthotel Helsinki City features laundry facilities, express check-in, and express check-out. Public areas are equipped with complimentary wireless Internet access.
Forenom Aparthotel Helsinki City is a smoke-free property.
- Total number of rooms - 95
- Elevator/lift
- Express check-in
- Express check-out
- Front desk (limited hours)
- Free WiFi
- Laundry facilities
- Smoke-free property
Family Friendly Amenities
- Family Hotel
- Free Wi-Fi
- Laundry facilities
- Refrigerator
- Microwave
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Room Amenities
- Refrigerator
- Coffee/tea maker
- Private bathroom
- Microwave
- Premium bedding
- Weekly housekeeping provided
- Shower only
- Hair dryer
- Flat-panel TV
- Free WiFi
- Cribs/infant beds (surcharge)
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
- In-room accessibility
Hotel Policies
Check-in time starts at 4 PM
Minimum check-in age is 18
Check-in time ends at midnight
Special check-in instructions:
Check-out time is 11 AM
Payment types
Children and extra beds
- Children are welcome.
- Kids stay free! One child 5 years old or younger stays free when using existing bedding.
- Rollaway/extra beds are not available.
- Cribs (infant beds) are available for EUR 50 per stay.
- Pets allowed for an extra charge of EUR 90 per accommodation (varies based on length of stay)
- 1 per room
- Specific rooms only
- Pet-friendly rooms can be requested by contacting the property at the number on the booking confirmation
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- No onsite parking is available.
No rollaway/extra beds available
Please note that this hotel does not accept Visa Electron as a form of payment. For more information please contact the property using the details on the booking confirmation.
Optional extras
- Pet fee: EUR 90 per accommodation (varies based on length of stay)
- Early check-in fee: EUR 9.90
- Late check-out fee: EUR 9.90
- Crib (infant bed) fee: EUR 50 per stay
Hotel Name
- Eerikinkatu
- Omena Eerikinkatu
- Omena Helsinki Eerikinkatu
- Omena Hotel Eerikinkatu
- Forenom Aparthotel Helsinki City Hotel
- Forenom Helsinki City Aparthotel Hotel
- Forenom Aparthotel Hotel
- Forenom Helsinki City Aparthotel
- Forenom Aparthotel
We should mention
One child 5 years old or younger stays free when occupying the parent or guardian's room, using existing bedding. This property allows pets in specific rooms only (surcharges apply and can be found in the Fees section). Guests can request one of these rooms by contacting the property directly, using the contact information on the booking confirmation.


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SAN FRANCISCO (AP) EBay said Tuesday that even as third-quarter net income dipped 29%, its revenue rose largely because more people and merchants are using PayPal to pay for things online.
The San Jose, Calif.-based company said the number of active PayPal accounts rose 19% to 78 million compared with a year earlier. That contributed to a 15% revenue jump to $688.1 million in its payments business — which includes PayPal and short-term credit service Bill Me Later.
But eBay's core marketplaces business, which includes the main eBay site and other sites like Shopping.com, saw revenue dip 1% to $1.4 billion.
Although the company attributed this decline partly to unfavorable foreign exchange differences, the decline in marketplaces may indicate that efforts to revitalize eBay's main site still haven't gained enough traction among bargain-hungry shoppers, or that shoppers have simply continued to rein in their spending due to the still-weak economy.
The online marketplace operator's third-quarter net income fell partly because operating expenses climbed. EBay earned $350 million, or 27 cents a share, compared with net income of $492 million, or 38 cents a share, in the year-ago quarter.
Revenue rose 6% to $2.24 billion.
Shares in eBay (EBAY), which also forecast third-quarter results in line with analyst estimates, fell $1.31, or 5.2%, in after-hours trading. The shares had finished regular trading down 3 cents at $25.03.
EBay said that the number of active users on its marketplace rose 2% to 89.2 million, showing that it is continuing to attract new buyers and sellers.
Its gross merchandise volume, which is a metric that indicates the value of all the items sold on eBay, excluding vehicles, rose too, gaining 7% to $12.2 billion.
Revenue from Internet communications service Skype, which is eBay's smallest business, increased 29% to $185.2 million.
EBay is planning to sell the majority of Skype to a group of private investment funds for $1.9 billion in cash and $125 million to be paid later. The company plans to keep the other 35%.
The deal may be complicated by ongoing litigation with Skype's founders, though, as they have filed a copyright-infringement suit against eBay and Skype alleging Skype violated an agreement over the use of technology that Skype licenses from another company founded by Skype's creators.
For the quarter that ends in December, eBay expects earnings of 28 cents to 30 cents a share, or 38 cents to 40 cents a share when excluding one-time items. The company expects revenue of $2.2 billion to $2.3 billion.
Analysts are looking for adjusted earnings of 40 cents a share on $2.26 billion in revenue.
Copyright 2009 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
Guidelines: You share in the USA TODAY community, so please keep your comments smart and civil. Don't attack other readers personally, and keep your language decent. Use the "Report Abuse" button to make a difference. Read more.


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Yes, I know it's a rhetorical question but she continues to astound with her dumbness.
Yes, I know it's a rhetorical question but she continues to astound with her dumbness.
At least she's hot.
Matt, I gotta wholeheartedly disagree with ya on that, but I will agree with Chip's statement.
Help stamp out, eliminate, and do away with redundancy.
Not dumb enough to realize she can't perform live very well.
Nice to see that she dressed up for the event.
I agree with Macro and Chip. I'll pass.
Some people play baseball. Baseball plays Jay Bruce.
does it matter?
What are you, people? On dope? - Mr Hand
I usually throw stuff at the TV when she shows up on a commercial during football. Can't stand her....hate the way she talks out of the side of her mouth and watching her perform is even worse than just hearing her perform. Her vocal performances are to entertainment are what Elaine Benes is to dancing
While dumb, she's not dumb enough to date me, yet. Therefore...
Nice body, but with pilates, personal trainers, and Dr. 90210, nice bodies are a dime a dozen. I think she has a weird chin, giving her the appearance of a drag queen from the neck up. She is very much a Barbie doll -- manufactured plastic, empty on the inside.
"I prefer books and movies where the conflict isn't of the extreme cannibal apocalypse variety I guess." Redsfaithful
Here it is. Flub comes at about 1:30 in the clip.
For every action there is an equal and opposite criticism.
Board Moderators may, at their discretion and judgment, delete and/or edit any messages that violate any of the following guidelines: 1. Explicit references to alleged illegal or unlawful acts. 2. Graphic sexual descriptions. 3. Racial or ethnic slurs. 4. Use of edgy language (including masked profanity). 5. Direct personal attacks, flames, fights, trolling, baiting, name-calling, general nuisance, excessive player criticism or anything along those lines. 6. Posting spam. 7. Each person may have only one user account. It is fine to be critical here - that's what this board is for. But let's not beat a subject or a player to death, please.


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By The Associated Press
For Boris Said, this week's NASCAR Nationwide Series event in Mexico City is more than just his first chance to race a stock car since February.
The free spirit from Southern California loves the sprawling city south of the border.
"I've enjoyed traveling the world to race cars, but I also have fun taking in the local sights and experiencing the local culture," he said. "As soon as I get to Mexico City, I look for the street vendor taco stands and order a couple of carne asadas (grilled steak). It is absolutely delicious - I recommend it to all Mexican food lovers."
More important, Said, a top road racer, will drive the No. 25 Ford Fusion in Sunday's race on the Autodromo Hermanos Rodriguez road course and will be aiming to win for the first time on the Nationwide circuit - previously known as the Busch Series.
He has finished in the top-five at the last three Nationwide races in Mexico, finishing fifth, second and third.
"This could be the year of the win," noted Said, who has a NASCAR Craftsman Truck Series victory. "I have a great crew and great equipment. My only concern is that I haven't been racing for a while."
The last time Said raced was in mid-February at the 150-mile qualifying event at Daytona International Speedway.
"I'm chomping at the bit to go racing again," he said. "I'm always pumped about my next race, but I am even more inspired for Mexico after watching the Rocky Balboa effort by Masters champion Trevor Immelman. To me, winning a NASCAR race is like winning the Masters."
COMING BACK: Longtime IRL IndyCar Series driver Davey Hamilton has signed with Kingdom Racing to drive in this year's Indianapolis 500.
Hamilton, who came back to racing after undergoing 21 operations and two years of rehabilitation following a devastating crash at Texas Motor Speedway in June 2001, finished ninth at Indy last year, his ninth start in the May race. He best Indy finish was fourth in 1998.
"I will do anything I can to race at Indy," Hamilton said. "I continue to train harder and am putting myself in the best physical shape of my life, because the most important race in the world is the Indianapolis 500. I'd like to thank Kingdom Racing for giving me that opportunity, and I look forward to a great partnership with them."
The new team is owned by Houston businessman George Del Canto, who hopes the Indy 500 entry will attract sponsorship for the 2009 IndyCar season.
LOOKING TO EXPAND: NASCAR Sprint Cup star and 2007 Daytona 500 winner Kevin Harvick and his wife, DeLana, have teamed with NHRA drag racing veteran Bob Vandergriff Jr. to explore fielding a Top Fuel dragster in NHRA competition.
The proposed Harvick-Vandergriff Motorsports would offer its sponsors marketing opportunities in NHRA, NASCAR Sprint Cup, Craftsman Truck and the Nationwide Series.
"The response from the companies we've spoken to already has been tremendous," Harvick said. "We view this as something that would be a natural extension of our current Craftsman Truck and Nationwide Series programs. Bob's a proven leader and innovator, and DeLana and I are happy to be here as his guests."
Vandergriff and Harvick hope to begin assembling a team and all the necessary components and people within the next two months as they prepare for a 2009 debut.
The Associated Press


text: Want to create more jobs? No new taxes! By on January 16, 2010 LEVINE: A SMALL BUSINESSMAN'S VIEW OF JOBS These days, it seems that every elected official, regardless of party affiliation, talks about the need for jobs. Some in passing and some at length, but, either way, it's jobs, jobs, jobs. And, after all, who could argue with that? Unfortunately, not everyone fully understands what it takes […]This content is only available to subscribers. Login or Subscribe Share this:EmailTwitterFacebook


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Advertisement [CLICK THE BUTTON ON THE RIGHT TO CONTINUE]
Author | exchange4media News Service | Wednesday, Mar 19,2008 8:25 AM
Times Internet Ltd (TIL), the company behind Internet portal Indiatimes.com, has awarded its media account to ZenithOptimedia Delhi. The business was earlier handled by GroupM's MEC. The realignment follows Times Internet's decision to look for a fresh perspective on all its brands.
The business includes TIL's flagship brand Indiatimes.com with its various sub-brands, Mail, Shopping, Travel and the mobile short code 58888, among others, besides the new services that are to be launched. One of the key things that swung the decision in ZenithOptimedia's favour was their ability to demonstrate a 3D media view that mirrors the changing media consumption habits, especially for the Internet generation, a large part of TIL's audience.
Commenting on the development, Dinesh Wadhawan, MD & CEO, Times Internet Ltd, said, "We are in acceleration mode and in the process of revamping our existing Internet brands as well as launching new ones. ZenithOptimedia displayed a fresh perspective on effective use of media for a 'Media' brand. They also bring with them a great ability to forge cross-media alliances that not only help in brand building but also increase revenue opportunities for our media offerings."
Speaking on the new business, Ambika Srivastava, CEO, ZenithOptimedia, said, "We are very excited to get the opportunity to work for Indiatimes and are looking forward to a long term relationship with them. These client wins are testimony to ZenithOptimedia's skills across product categories, extensive knowledge of the industry, state-of-the-art proprietary research tools, and proven buying efficiency."
TIL has been one of the Internet companies that have been quite active in the advertising space. Its relaunched offering of Indiatimes Mail, backed by a large television campaign, has seen a multi-fold growth in new account registrations.


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Program Time: To purchase airtime for ethnic or English language programming call the Program Director, Lucyna Migala, at (773) 282-6700 or e-mail her at email@example.com.
Advertising: To purchase advertising call (773) 282-6700 or contact the station by mail at 5356 West Belmont, Chicago IL 60641-4192 or write to us via e-mail at email@example.com.
Public Service Announcements:
WCEV serves the community by airing Public Service Announcements for non-profit and not for profit organizations.
Public Service Announcements should be sent in BY MAIL ONLY
at least threeweeks prior to the event in air-ready text fashion to:
5356 West Belmont Avenue
Chicago, Illinois 60641-4192
Interviews / Who We Are: WCEV features a locally produced interview program on topics of interest to Ethnics and all Chicagoland listeners called "Mosaic." It also airs a feature called "Who We Are" featuring information about Ethnic Americans and all Americans. For further information about these items call or e-mail the Program Director, Lucyna Migala, at (773) 282-6700 or firstname.lastname@example.com.


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Malfa Vacation Packages
Malfa is located some 260 miles (418 kilometers) from Rome, the capital of Italy.
This region is a firm fixture on the tourist trail. To discover why, explore the other places around Malfa. While in the area, take a trip to Leni. It is 2 miles (3 kilometers) away from Malfa and is home to the Saint Joseph's Parish and Port at Rinella. If you feel like even more, travel to Santa Marina Salina and enjoy the Church of Santa Marina Salina and Port of Santa Marina. Santa Marina Salina is approximately 2 miles (3 kilometers) from Malfa.
Grab some transportation and visit Messina, a regional center, some 45 miles (72 kilometers) from Malfa. The place offers a range of visitor attractions, for example Sanctuary of Montevergine, Fountain of Orion (Fontana di Orione) and Regional Museum of Messina (Museo Regionale). University of Messina, Palazzo Calapaj and Sant'Elia Church are just a few of the other interesting attractions on offer here.
Get outdoors and enjoy Sicily's popular open-air markets, open-air museums and gardens. No matter what your spiritual beliefs, visitors are sure to enjoy at Sicily's cathedrals and other holy sites.
There are many ways for thrill seekers to enjoy the wide open spaces around Malfa. Horse riding and mountain climbing are just two popular ways thrill seekers here can get their kicks. Take a big breath and de-stress as you have a go at this region's more relaxing pastimes such as bird watching and sunbathing.
You'll be reluctant to bid "Ciao" (farewell) to this interesting place.


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What's it like to live in Barrow-In-Furness?
Is there much greenery in the area? What are the local amenities like?
Asked on Apr 2 2008, General in Barrow-in-Furness | Report content
Answers (3)
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Living in Barrow is strongly dependent on where you live in Barrow, two estates standout as being the better places to live, Hawcoat and Holbeck being my first choices, the rest of Barrow seems to be occupied by people who are struggling a little more with the recent increase in living costs, their is a huge rise in house sales. basically put Barrow has a bad reputation which I can't say I agree with, its a nice place to live, I would just try and avoid the council estates, which is pretty much the same anywhere you go.Answered on Feb 24 2013, Report content
There are many green areas. There is a huge park that has a fairly big pond. There is an Abbey with some lovely walks. We are right on the coast, so have some fab beaches...notably Roan Head and the Coast Rd. The amenities are dependent on age, there are a handful of great restaurants, a smattering of good restaurants and too many fast food places. There are plenty of activities for toddlers and younger children. Teenagers have a variety of activities such as skate park and shopping in Tesco most evenings...there is the cinema!Answered on Apr 23 2013, Report content
Hi, We would suggest that you get in touch with local estate agents in order to get some background about the area. You can search for agents using the 'find agents' tab on the top of the Zoopla Page. We hope this helps.Answered on Oct 1 2013, Report content
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An entrafield was a security and privacy device used in residences on planet Drema IV. The entrafield enclosed the entire home as well as selected rooms within, and could block scent and sounds within certain acoustic ranges. (SCE eBook: Progress)
- This is presumably the seemingly holographic door seen in the TNG episode: "Pen Pals".


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Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors
Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain.
Recombinant AAV-Cre, AAV-GFP (green fluorescent protein) and LV-Cre-EGFP (enhanced GFP) were made with the transgene controlled by the cytomegalovirus promoter. Infecting 293T cells in vitro with AAV-Cre and LV-Cre-EGFP resulted in transduction of most cells as shown by GFP fluorescence and Cre immunoreactivity. Injections of submicrolitre quantities of LV-Cre-EGFP and mixtures of AAV-Cre with AAV-GFP into the neocortex and hippocampus of adult Rosa26 reporter mice resulted in strong Cre and GFP expression in the dentate gyrus and moderate to strong labelling in specific regions of the hippocampus and in the neocortex, mainly in neurons. The pattern of expression of Cre and GFP obtained with AAV and LV vectors was very similar. X-gal staining showed that Cre-mediated recombination had occurred in neurons in the same regions of the brain, starting at 3 days post-injection. No obvious toxic effects of Cre expression were detected even after four weeks post-injection.
AAV and LV vectors are capable of delivering Cre to neurons in discrete regions of the adult mouse brain and producing recombination.
Mice carrying conventional null mutations of genes have been very useful in studying the roles of various molecules in the development and functions of the nervous system. However, the possibility of compensatory changes during development is often raised as an explanation for the very limited phenotypic abnormalities observed in some knockout mice. Furthermore conventional null mutations of genes, which have important functions during development often show an embryonic lethal phenotype, making experiments on adult animals impossible. The ability to manipulate the genotype in vivo provides major opportunities for studying gene function in the mammalian nervous system and for developing novel therapeutic strategies
Over the last few years, development of the Cre-loxP system, which is concerned with recombination mechanisms in the bacteriophage P1, has provided powerful technologies for the manipulation of genes. Cre is a 38 kDa recombinase and loxP is a Cre-specific recognition sequence present in bacteriophage DNA, to which the recombinase binds. This results in the excision or inversion of stretches of DNA flanked by loxP sites, depending on the orientation of the loxP sequences
Several strains of mice exist which have been engineered to express Cre under the control of promoters with various tissue specificities. These can be bred with animals carrying loxP-flanked genes to produce gene inactivation in different tissues. However, there are clear advantages to being able to deliver Cre to specific groups of neurons in adult animals. We describe here the construction of replication deficient adeno-associated virus (AAV) and lentivirus (LV) vectors expressing Cre (AAV-Cre and LV-Cre-EGFP) and the efficiency of transduction, effects on cell survival and efficiency of recombination in Rosa26 reporter mice, 3 days and 1, 2 and 4 weeks following stereotactic injections of the vectors into the neocortex and hippocampus. Rosa26 mice carry a copy of the lacZ (β-galactosidase) gene as a reporter for Cre activity, controlled by the Rosa26 locus. Expression of the gene is activated by Cre-driven removal of a stop-cassette, which consists of the neo-expression cassette followed by three pA sequences, flanked by loxP sites
Viral vectors deliver Cre to a cell line
The primary objective of this part of the study was to determine whether the LV-Cre-EGFP and AAV-Cre viruses allow the delivery of Cre recombinase to mammalian cells. 293T cells were infected either with LV-Cre-EGFP or AAV-Cre viruses. Cells infected with LV-Cre-EGFP were analysed for GFP fluorescence and immunohistochemistry for Cre with Cy3 conjugated secondary antibodies, 16–48 hours after infection. Green fluorescence was detected in the nucleus of many cells and colocalized with Cre (Fig.
Figure 1
(A, B) EGFP fluorescence (A) and Cre immunoreactivity (B) in HEK 293T cells infected with LV-Cre-EGFP recombinant virus in vitro
(A, B) EGFP fluorescence (A) and Cre immunoreactivity (B) in HEK 293T cells infected with LV-Cre-EGFP recombinant virus in vitro. All the cells which express EGFP also express Cre. (C, D) Immunohistochemistry for Cre, in HEK 293T cells 16 hrs after exposure to the AAV-Cre virus (1C) and in uninfected HEK 293T cells (1D). Cre is present in the nucleus of large numbers of cells infected with the virus but is absent from uninfected cells. Scale bars A and B = 25 μm, C = 50 μm, D = 100 μm.
Virally-delivered GFP is expressed in the adult mouse brain
To verify the efficiency of gene delivery to the brain using our viral vectors, GFP immunohistochemistry was performed on coronal sections of Rosa26 mouse brain 4 weeks after injection of either a mixture of AAV-Cre and AAV-GFP or of LV-Cre-EGFP viruses (Fig.
Figure 2
Immunohistochemistry for GFP after infusing LV-Cre-EGFP virus (A, B) and AAV-Cre/AAV-GFP mixture viruses (C, D) into the neocortex and hippocampus of Rosa26 mouse brain
Immunohistochemistry for GFP after infusing LV-Cre-EGFP virus (A, B) and AAV-Cre/AAV-GFP mixture viruses (C, D) into the neocortex and hippocampus of Rosa26 mouse brain. Very strong GFP expression is present in the molecular layer and polymorphic layer (arrows in B and D) of the dentate gyrus. GFP positive cells are present in CA1, CA2 and terminal part of the CA3 region of the hippocampus (A, C). Strong staining was also observed in presumptive glial cells in the subcortical white matter and corpus callosum (arrows in A and C). Fig.
Most cells in mouse brain which express virally-delivered GFP also express Cre
In order to make sure that GFP expression was a good marker for Cre expression in infected cells following LV-Cre-EGFP and AAV-Cre/AAV-GFP mixture injection into Rosa26 mice, immunofluorescence images of sections immunoreacted for both GFP and Cre were compared (Fig.
Figure 3
Double immunofluorescence for Cre and GFP using Cy3 and FITC labelled secondary antibodies respectively with single excitations of 543 nm to detect Cre (A, C) or 488 nm to detect GFP (B, D)
Double immunofluorescence for Cre and GFP using Cy3 and FITC labelled secondary antibodies respectively with single excitations of 543 nm to detect Cre (A, C) or 488 nm to detect GFP (B, D). LV-Cre-EGFP virus (A-B) and AAV-Cre/AAV-GFP mixture viruses (C-D) were injected into the hippocampus and neocortex of Rosa26 mice 4 weeks before perfusion. The patterns of expression of the two transgenes are almost identical (allowing for the slightly stronger staining obtained with the tyramide-enhanced Cre immunohistochemistry) irrespective of the vector used. Fig.
Absence of toxic effects of gene delivery with viral vectors
No signs of cavity formation or other obvious tissue damage were found in regions where GFP or Cre were detected following injection of either AAV-Cre/AAV-GFP mixture, or LV-Cre-EGFP viruses into mouse brain. Individual neocortical neurons infected with AAV-Cre/AAV-GFP mixture and LV-Cre viruses could be identified and they retained their normal morphology up to 4 weeks after injection of the vectors.
Virally-delivered Cre produces recombination in adult mouse brain
To show that the Cre recombinase delivered to mouse brain was functional, the pattern of Cre-mediated recombination was studied in adult Rosa26 reporter mice, injected with either LV-Cre-EGFP or AAV-Cre/AAV-GFP mixture. Rosa26 cells express β-galactosidase following Cre-induced recombination. We performed β-galactosidase enzyme histochemistry, 3 days and 1, 2 and 4 weeks following the stereotactic injection of LV-Cre-EGFP and AAV-Cre/AAV-GFP mixture into the neocortex and hippocampus. These two regions were selected to establish the potential of viruses to deliver functionally active Cre to different cell types. β-galactosidase activity was detected in dentate gyrus at all studied time points, but was maximal 2 and 4 weeks following viral injections into the cortex and hippocampus (Figs.
Figure 4
Cre-mediated recombination following injection of LV-Cre-EGFP (A-C) and a mixture of AAV-Cre and AAV-GFP recombinant viruses (AAV-Cre/AAV-GFP mixture) (D-F) into the neocortex and hippocampus of Rosa26 reporter mice
Cre-mediated recombination following injection of LV-Cre-EGFP (A-C) and a mixture of AAV-Cre and AAV-GFP recombinant viruses (AAV-Cre/AAV-GFP mixture) (D-F) into the neocortex and hippocampus of Rosa26 reporter mice. Expression of β-galactosidase, indicating Cre-mediated excision of the loxP flanked stop signal, was detected by the X-gal staining. β-galactosidase activity was prominent in the dentate gyrus of animals at all postinjection intervals (arrows in A-F). Scale bars A= 100 μm (applies also to B-F).
Figure 5
Cre-mediated recombination in the neocortex and hippocampus of Rosa26 reporter mice, 4 weeks following injection of LV-Cre-EGFP recombinant virus (A-C) or a mixture of AAV-Cre and AAV-GFP recombinant viruses (AAV-Cre/AAV-GFP mixture) (D-F)
Cre-mediated recombination in the neocortex and hippocampus of Rosa26 reporter mice, 4 weeks following injection of LV-Cre-EGFP recombinant virus (A-C) or a mixture of AAV-Cre and AAV-GFP recombinant viruses (AAV-Cre/AAV-GFP mixture) (D-F). β-galactosidase activity is apparent in and around the needle track in the neocortex (arrows in A and D), in parts of the CA1, CA2 and terminal portion of the CA3 region of the hippocampus and in the dentate gyrus. Cells in stratum lacunosum-moleculare (arrowheads in A and D), corpus callosum (arrow in B and E) were also observed. Parts of CA1 and CA2 from Fig.
Injections of LV-Cre-EGFP and AAV-Cre/AAV-GFP mixture viruses into neocortex and hippocampus also resulted in recombination in glial cells, most obviously in the white matter of the subcortical white matter and corpus callosum (arrows in Fig.
We have successfully used AAV and LV to deliver Cre recombinase to a mammalian cell line in vitro and to cells in the brains of Rosa26 mice 3 days to 4 weeks after stereotactic injection in the neocortex and hippocampus. The AAV and LV vectors produced stable transduction of large numbers of neural cells in the regions injected and produced recombination in those cells without obvious tissue damage. These vectors can therefore be used to inactivate genes in discrete regions of the adult mammalian brain and offer exciting possibilities for studying gene function in the nervous system.
Our study extends the recently published studies, which have shown that AAV, LV and adeno virus expressing Cre protein can mediate loxP-dependent recombination
Both neuronal and glial cells were transduced by both AAV and LV vectors, as shown by their expression of immunohistochemically detectable Cre and GFP, but neurons were the most abundant transduced cells with both viral vectors. Transduction of glial cells was most obvious in the subcortical white matter. AAV and LV vectors using the CMV promoter have been reported to produce transgene expression predominantly in neurons but to a lesser extent also in glia
Recombination, detected by β-galactosidase expression, was found in the same populations of cells as expressed GFP and/or Cre. Hence, recombination was predominantly in, but not restricted to, neurons and in the hippocampal formation recombination was most obvious in dentate gyrus neurons and scarcely detectable in neurons within most of CA3. β-galactosidase activity was detected in dentate gyrus neurons 3 days after injection of LV-Cre-EGFP and AAV-Cre/AAV-GFP mixture, although maximal expression of β-galactosidase was found at 14 days or more. AAV vectors carrying erythropoetin have been reported to produce expression of the transgene in striated muscle one day after infection
Our data show that Cre can be effectively delivered to discrete regions of the adult mammalian brain using AAV and LV vectors. This offers the potential for producing changes in the genome of neurons in discrete regions of the brain at the time of choice. The limiting factor in exploiting this technology is likely to be the generation of strains of mice with loxP-flanked genes of interest to neuroscience.
The AAV vector pTRCGW (5.6 kb), (a kind gift from Paul Dijkhuizen, The Netherlands Institute for Brain Research, Amsterdam, The Netherlands), carrying the ampicillin resistance gene, inverted terminal repeats (ITR), human immediate early cytomegalovirus promoter (CMV), wood chuck posttranscriptional regulatory element (WPRE) and multiple restriction sites, was used to construct the rAAV-Cre vector plasmid. The 1.1 Kb fragment of Cre gene along with the nuclear localization signal (NLS) was excised from pNSE-Cre
To generate the LV-Cre-EGFP plasmid, a 2 Kb NotI/BglII fragment containing Cre-EGFP was isolated from pCMV-Cre-EGFP, blunted, and ligated into the multiple cloning site of the lentiviral transfer vector p156RRLsin-PPThCMV-GFP-pre
AAV-Cre Viral vector production
Ten culture dishes (100-mm diameter), each containing 5 × 106 293T cells, were co-transfected by calcium phosphate with 5 μg of either AAV-GFP or AAV-Cre vector plasmid and 15 μg of the packaging plasmid(PDG)
To determine if Cre was expressed in AAV-Cre infected cells in vitro, 293T cells were plated in 6 well plates (5 × 105 per well) in complete DMEM. Cells were infected with AAV-Cre (1:10,000) in the presence of adenovirus (MOI = 3). Fourteen-forty eight hrs following the infection, cells were washed with PBS and fixed with 4% PFA for 15 min, followed by washing with PBS (3 × 10 min). Cells were then incubated with antibody to Cre (1:200, Berkeley, CA, USA), diluted in 50 mM Tris-HCl, pH 7.5, 0.5 M NaCl and 0.1% Triton) overnight at 4°C. The following morning, cells were washed with PBS (3 × 5 min) and incubated with ABC solution (Avidin Biotin Complex, Vector laboratories, CA USA) for an hour at room temperature. The cells were washed with PBS (3 × 5 min) and allowed to react for 5 min in a solution of 0.05% diamino benzidine and 0.03% hydrogen peroxide in PBS. The cells were washed with PBS to terminate the reaction and subsequently observed under a bright-field microscope (Zeiss Axiovert 10).
LV-Cre-EGFP viral vector production
For viral vector production, the LV-Cre-EGFP transfer plasmid was co-transfected together with the viral core packaging construct pCMVdeltaR8.74 and the VSV-G envelope protein vector pMD.G.2 into 293T cells as previously described
To determine if Cre was expressed in LV-Cre-EGFP infected cells in vitro, 293T cells (5 × 105 per well) were plated in complete IMDM. The next day, cells were infected with LV-Cre-EGFP (1:10,000) in complete IMDM medium for 16 hrs, medium was replaced with fresh medium and incubated for 24–48 hrs. These experiments were replicated 3 times. For immunofluorescence labelling, cells were washed with PBS and fixed with 4% paraformaldehyde for 15 min. followed by washing with PBS (3 × 10 min). Cells were then incubated with the Cre antibody (1:200, diluted in 50 mM Tris-HCl, pH 7.5, 0.5 M NaCl and 0.1% Triton) overnight at 4°C. Next morning cells were washed with PBS (3 × 10 min) and incubated with Cy3-conjugated goat anti-mouse (1:400, Jackson laboratories PA, USA) for an hour at room temperature. Cells were washed with PBS (3 × 10 min), mounted on slides and observed under a confocal microscope (LSM 410 Zeiss) using 488 nm and 543 nm excitations for GFP and Cy3 respectively.
AAV and LV delivery of Cre into the brain
Rosa26 reporter mice (22–30 g) were deeply anaesthetised by a fluanisone /fentanyl mixture containing Hypnorm (Janssen Pharmaceutical, UK) and Dormicum (Roche, NL) and water in a ratio of 1:1:2 (10 μl/gm I.P.). Virus was injected into the cortex and hippocampus via 30G stainless needle attached by polyethylene tubing to a 10-μl Hamilton syringe and a Harvard 22 microinjection pump. 0.8 μl of a mixture of AAV-Cre and AAV-GFP (AAV-Cre/AAV-GFP mixture, 9:1) or 0.5 μl LV-Cre-EGFP virus was injected into the cortex (anterioposterior -2, lateral + 1.3, dorsoventral -0.45) and hippocampus (anterioposterior -2, lateral + 1.3, dorsoventral -1.55)
β-galactosidase staining was done by incubating the sections for an hour at 37°C with X-gal-solution containing 5 mMK4Fe(CN)6, 5 mM K3Fe(CN)6, 2 mM MgCl2, 1 mg/ml 5-bromo-4-chloro-3-indolyl-D-galactoside in PBS. The X-gal reacted sections were washed with PBS (3 × 10 min), dehydrated and embedded in entellan mounting medium. Immunohistochemistry for GFP was carried out by the ABC method using diamino-benzidine as a substrate
Colocalization of Cre and GFP was examined by using a double immunofluorescence. A mixture of biotin labelled monoclonal Cre antibody (1:1000) and polyclonal GFP antibody (1:100) was used as primary antibodies, while a mixture of streptavidin-conjugated horseradish peroxidase (1:200, Life Sciences, MA, USA) and FITC conjugated anti-rabbit (1:100, Sigma) was used as secondary antibodies. Cre signal was enhanced by incubating the sections in fluorophore labelled tyramide solution (1:200, Life Sciences) for 30 min at RT, mounted sections were analyzed by fluorescence microscope fitted with SP confocal head (Leica DMRE) using Leica confocal software.
Author's contributions
BYA generated the Cre vectors in collaboration with WTJMCH and SN under the supervision of JV. The Cre-EGFP fusion construct was cloned and the Cre reporter mice provided by CL. The in vitro and in vivo studies were carried out by BA in collaboration with RE and SC. The histological work was carried out by BYA and PNA. ARL, FS, JZ and PNA participated in the design of the study and performed part of the histological analysis. FS, JV, PNA and ARL conceived of the study, and participated in its design and coordination. All authors read and approved the final manuscript.
We thank Anthony J.G.D. Holtmaat for helpful suggestions. This work was supported by a grant from Wellcome Trust, grant number 060367.
Subregion- and cell type restricted gene knockout in mouse brain
Cre-mediated transgene activation in the developing and adult mouse brain
Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity
Adeno-associated viral vectors for gene transfer and gene therapy
Lentiviruses as gene transfer agents for delivery to non-dividing cells
Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery
Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector
Gene transfer by adeno-associated virus vectors into the central nervous system
Differential and persistent expression patterns of CNS gene transfer by an adeno-associated virus (AAV) vector
Long-term gene expression and phenotyping correction using adeno-associated virus vectors in the mammalian brain
Targeted gene disruption: applications in neurobiology
Studies on the properties of P1 site-specific recombination: Evidence for the topologically unlinked products following recombination
Targeted oncogene activation by site-specific recombination in transgenic mice
Generation of a mouse strain that produces immunoglobulin kappa chains with human constant regions
Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting
Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting
Delivery of the Cre recombinase by a self deleting lentiviral vector: Efficient gene targeting in vivo
Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells
Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids
Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome
Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance
Multiplex Cre/lox recombination permits selective site-specific DNA targeting to both a natural and an engineered site in the yeast genome
Mammalian genomes contain active recombinase recognition sites
Generalized lacZ expression with the Rosa26 Cre reporter strain
Adeno-associated virus effectively mediates conditional gene modification in the brain
Targeting conditional gene modification into the serotonin neurons of the dorsal raphe nucleus by viral delivery of the Cre recombinase
Focal deletion of the adenosine A1 receptor in adult mice using an adeno-associated viral vector
Adenovirus-mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion
Analysis of C-MYC function in normal cells via conditional gene-targeted mutation
Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system
Differential efficiency of adenovirus-mediated in vivo gene transfer into skeletal muscle cells of different maturity
Structure of adeno-associated virus vector DNA following transduction of the skeletal muscle
Lentiviral transduction of green fluorescent protein in retinal epithelium: evidence of rejection
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences
Subregion- and cell type restricted gene knockout in mouse brain
Novel tools for production and purification of recombinant adenoassociated virus vectors
Purification of recombinant adeno-associated virus by iodixanol gradient ultracentrifugation allows rapid and reproducible preparation of vector stocks for gene transfer in the nervous system
Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector
Atlas of Mouse brain, 2nd edition
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures


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De Broglie's relationship
As described above, radiations have to be considered as wavelike in order to explain certain experiments and also as particle to explain certain other experiments. This dual behaviour is commonly referred to as wave-particle duality.
In 1924, Louis de Broglie proposed that if light can display this wave-particle duality, then matter, which certainly appears to be particle like, might also have wavelike properties under certain conditions. Louis de Broglie was able to put this idea quantitatively by deriving a relationship between wavelength λ (characteristic of wave) and momentum p (characteristic of particle).This relation may be derived as follows:
Planck's relation
E = hγ = hc/λ
Einstein's relation
E = mc2 Equating these two expressions, we get mc =
Now, the term mc in the above expression may be considered to be the momentum p of the photon. Thus, we have
p =
For ordinary material particles, de Broglie suggested that c may be considered to be equivalent to v (i.e. their velocities) and thus it is also applicable to the material particles.
According to the above equation, particle and wave natures are inversely related to each other. If the mass of the particle is small, as in the case of subatomic particles, the wavelength is significant, i.e. for such particles, wave nature is expected. For a particle of larger mass, the wavelength is too small to be of any practical significance.


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Euthanasia campaigner Philip Nitschke's campaign to win back his registration to practise medicine is in disarray following the sudden death of his pro bono counsel and fellow activist Peter Nugent.
In an email to Fairfax Media, Mr Nitschke said that Mr Nugent had been suffering stage four cancer, and "was well prepared, and had options in place" for ending his own life. However, he had been responding well to chemotherapy, seemed to be in a good state of based on their communications, was excited about the upcoming court case and suicide seemed unlikely.
He described Mr Nugent's death as a "shock", "perplexing" and "a great loss".
Mr Nugent was preparing for an April hearing in the Supreme Court in Darwin to challenge Mr Nitschke's suspension by the Northern Territory Health Professional Review Tribunal in July last year. Mr Nitschke was struck off by the Medical Board of Australia after reportedly admitting to supporting a 45-year-old Perth man's decision to take his own life despite knowing the man was not terminally ill. The man, Nigel Brayley, wasn't a patient of the then Dr Nitschke.
The tribunal later supported the board's decision. The medical board has since added 12 charges that allege further professional misconduct.
Mr Nitschke said, "Given that suicide is not illegal, Peter [Nugent] argued that their decision was in breach of common law provisions, something the NT Tribunal panel of doctors either didn't realise or care about. Hence, the importance of having this reviewed in law, in the NT Supreme Court."
Regarding the additional charges, Nitschke said: "They are clearly unhappy that the Brayley decision is under legal scrutiny, and want to push ahead while they can with this new round of blatantly political charges."
Mr Nitschke, who heads the pro-euthanasia group Exit International, of which Mr Nugent was a member, said he was now scrambling to find another QC to act for him pro bono.
He said Exit International had raised about $200,000 through a crowdfunding appeal, but had "spent most of it with solicitor and incidental fees".
Regarding his own financial standing, he wrote: "The suspension hasn't affected my income, in fact it's probably increased it, with a sharp rise in sales of my Peaceful Pill Handbook, and membership of Exit."
Mr Nitschke was writing from the UK where he has "been preparing a show for the Edinburgh Comedy Festival on euthanasia (and the antics of the Medical Board – which lends itself well to comedy)."
These plans, he said, have been put on hold. "I'll have to come back to [Australia and] try to sort out this mess."
In any event, he said, the show will run in the Melbourne Comedy Festival in 2016.


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Téodor Achewasser T-Shirt
Inspired by an old Bavarian bierdeckel. The bear carries a huge mug of beer which implies joy and wonderful times. Wear this shirt in order to spread happiness to all who see you. Text translates to, "American Harming-water." "Stammtisch" roughly translates to, "a drinking table reserved for regulars."Printed on the American Apparel 2001 Fine Jersey (size info)
type: tshirt vendor: Merchify.com


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I have a mac Server 2011 and I been trying to setup Virtual Sites how do I do that ?
3 Replies
Mar 16, 2011 at 3:34 UTC
One way to do it would be to create multipe sites and diffrentiate them using port numbers or IP addresses.
You can set multiple IP addresses to one network card. After doing this, you create 2 sites and give them each one of the IP addresses each.
Or, Create 2 sites and give them 2 different port numbers. Than you will be able to connect to them with the port numbers. eg myserver.com:8080 for the first site and myserver.com:8091
There are many other ways like using Host files and headers etc, the above would be the simplest to start off with.
Mar 16, 2011 at 4:33 UTC
Hey medi man,
VIrtual Sites can exist on the same IP address so long as you make 100% sure that any alias for the sites are set in the Aliases tab. e.g. www.domain.com, domain.com, etc. Otherwise, you get rollover. We're doing this for several websites.
If you do SSL for any of the Virtual Sites you MUST dedicate an IP address for that website (inside and Internet). The IP Address can exist on the same physical network port as the default IP address, but requires a separate entry in the System Preferences > Network Pane > Click the "+" attach it to an existing port (e.g. Ethernet 1) > Name the connection > set the static IP address as normal. No need to set the gateway, DNS, etc since that's handled by the primary address.
I attached a photo. Not sure how much it'll help
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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A lot of news today out of New York City about protesters being arrested. My guess is that it will only get worse in the coming days.
One of my favorites is here - these kids were surprised to find that jail isn't a friendly place. Hmmm.
(The NYTimes has good stories too, but I skipped those links that require registration.)


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by Gina Lynn
I have been going through a extremely difficult time over the past few years after my divorce. Please pray for me and my sons to find peace and calm in our lives and to take away the drama and outside stresses.
Join in and write your own page! It's easy to do. How? Simply click here to return to Online Prayer Requests.
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Could anyone tell me when to use 'bù' and when 'méi'?
I mean, I learned that both mean something like 'not', as in BU SHUO, BU HUI, BU DONG etc.
But why is it MEI YOU, MEI TING, and probably some other examples?
Thanks in advance!
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This category is for articles that require Cleanup. The templates {{Cleanup|date={{subst:CURRENTMONTHNAME}} {{subst:CURRENTYEAR}}}}, {{list}} or {{verify}} place items here.
See GTA Wiki:Cleanup Templates for details on which cleanup templates to use, and Category:Cleanup Templates for a list of them.
Pages in category "Cleanup"
" Deleted Missions are missions that were cut before a game's release by developers. There are...
Deleted Missions


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Laser Lipolysis
Flaccidity, loss of definition and fat accumulation are the main aesthetic problems concerning the arms. The Laser lipolysis or Lipolaser technique can treat these imperfections in a safe and effective manner, in just one session.
Causes of aesthetic problems on the arms
- Ageing. The cellular metabolism decreases over the years. Generation of collagen and elastin fibres, responsible for supporting the skin and connecting it to the underlying tissue, is reduced and is generated at a lower frequency.
- Hormonal changes. Loss of elasticity is a symptom of the alterations menopause causes on the skin.
- Weight gain. The skin on the arms becomes deformed when the fat deposit is more than it can support.
The combination of these circumstances, added to the fragility of the inside area of the arms, gives rise on the one hand, to sagging fat and muscle mass, and on the other, to flaccidity, causing the "bat wing" effect or flabby skin.
Flaccidity and fat accumulation are the main aesthetic problems concerning the arms
Laser Lipolysis for Arms
Laser lipolysis is a minimally invasive, high precision technique that achieves two goals in just one procedure:
- Destruction of the localised fat deposits, without affecting the adjacent tissue.
- Skin contraction, to readjust the new shape in a natural way.
Skin contraction on the arms is extremely important for the result to be satisfactory.
At IML, Laser lipolysis is performed using the Aspire® platform, a device that emits at two types of wavelength: 942 nm, optimal for selective destruction of the adipose tissue and 975 nm, which causes skin contraction. This laser uses a fine and flexible cannula with an optical fibre on the end, which emits the laser energy.
What does the Laser lipolysis procedure on arms involve?
- It is performed under local tumescent Klein type anaesthetic. This solution provides an optimum vasoconstriction level to reduce bleeding.
- The physician adjusts the laser dosimetry, choosing the 924 nm wavelength.
- A small incision is made in one of the folds of the armpits, and the optical fibre is introduced, generating fat liquefaction as it comes into contact with the adipose tissue. Another incision may be required in some cases, also a very small one, in one of the folds of the elbow.
- The melted fat is removed by aspiration with a fine, 2 mm-thick cannula.
- The physician selects the 975 nm wavelength, which heats the water in the elastic fibres and the interstitial tissue, causing coagulation a collagen reorganisation, which is responsible for skin contraction.
- Once the procedure is complete, a compressive bandage is placed on the treated area.
- There is no need for admittance, so the patient can return home on the same day of the treatment. Recovery time is just 2 days.
What are the results achieved with Laser lipolysis for arms?
- Volume correction
- Flaccidity correction
- Better defined arm contours
- Natural and rejuvenated appearance
What other areas can be treated with Laser lipolysis?
Request a free informative consultation. One of our physicians will provide you with personalised information of the treatment that best suits your needs.