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|Dean Gaudet <dgau...@arctic.org>
|Re: NameVirtualHost
|Mon, 27 Oct 1997 22:52:35 GMT
On Mon, 27 Oct 1997, Rob Hartill wrote: > that doesn't mean to say that there was or wasn't a significantly > larger number of people not living on the limit and having no problems > with smaller numbers of vhosts. I don't think I ever had problems with > them before the recent changes. They just worked intuitively for the > way I intended to use them. I assume many other people had similar success. > Yes, fine, whatever. We disagree, let's leave it at that. Neither of us have any numbers to base these arguments on. I'm just telling you that I see bug reports and such indicating that there are definately a nontrivial number of people who are running a large number of vhosts. *This is one of Apache's huge selling points: that we easily support hundreds of vhosts.* You make it sound like I've broken things for small vhosts, I most certainly have not. There's no "edge" except for the #file-descriptors "edge", and that's completely unrelated. > My gut feeling is that people using smaller numbers of name based > vhosts outnumber the 'life on the edge' folks whose systems broke > because they were pushing things much harder and that the latter are > the exception rather than the rule. Which is why I suggested a very trivial change to NameVirtualHost at the beginning of this thread. > Maybe it won't work out more intuitive, but at the moment, yes it does > feel better to me, mainly because it avoids more nested <></> blocks > which eat too much space and are harder to look at. Not everyone is a perl programmer ... your syntax looks like something a perl programmer would write :) BTW, I hate the <> crap myself, but that's legacy. Having a consistent syntax is important, mixing two syntaxes is not a good idea imho. For example, we don't use colons for anything yet. What happens when the : is part of a path in your syntax? What happens (in your syntax) when we want to add something more than just name and path matching for non-ip vhosts? For example, what if there's some part of the SSL negotiation that can be used, which requires another bit of config info? Mine at least allows for a new directive within the inner-most container. > ok, here's where I think the proposal has evolved to, using 'Service' again. > > format: > > Service [name]:[ip]:[port]:[path] vhost-block-alias > > The config parser would read one Service line at a time and put the > results into tables; there would be 1 table per IP address. > Your ip-hashing would map an IP to a table. Ordering withing these > tables is important, the earlier in the table the earlier it is checked > for a match (the first to match wins). > > For every IP the server operates from, there would be one table > that lists [name]:[port]:[path] - the things we want to match against, plus > the vhost-block-alias which is a key to another hash/table that determines > which <VirtualHost></VirtualHost> contents are important. > > Any 'Service' line where an explicit IP is mentioned, the [name]:[port]:[path] > goes into the table at the next free slot. If a 'Service' line doesn't > mention the IP then [name]:[port]:[path] goes into ALL tables - this > makes sure that we always catch things like Brian's example ::79: where > we want to intercept all port 79 requests first and not worry about the > rest of the parameters (all IPs would catch it). all tables? You have no idea what all the tables are. You have no idea what all the ip addresses are that you'll see. You need to add an artificial "default" table. Note that doing as you suggest makes it really expensive to have a single port based vhost, it's entry has to take up space everywhere (this is not a new problem, we already have this problem). My proposal has a branch in the decision tree first at the port level -- and that level can be eliminated entirely. My hypothesis is that port-based servers are rare. > When a request arrives, we use the IP to choose the table and then work > through systematically looking for an entry that doesn't fail to match > on any criteria. The catch-all ':::' would be at the bottom of all tables to > guarantee success. > > In this scheme, someone with 500 IPs benefits from your hashing - they > lookup a table containing (probably) just the ':::' entry and they > get fast IP -> <VirtualHost></VirtualHost> mapping. > > For the places where hashing isn't an issue, we'll typically end up with > a small number (usually 1) of tables containg varying numbers of entries. > The 'n:p:p' entries give full control over how to unambiguously map a > request to the right vhost configs. The ordering (as Brian showed) > enables us to pick off special cases first, or (as I showed) to list the > most likely match first (e.g. a popular hostname comes before an infrequently > used one). I still don't think the complexity of Brian's suggestion is necessary. Longest suffix match (my suggestion) for hostnames is easy to make go fast, but this isn't a negative for either yours or my proposal. It's just a simplification. > The 'Service' lines separate the parameter matching from the <VirtualHost> > </VirtualHost> actions. I think that separation is very useful (not least > for mapping multiple criteria to the same <VirtualHost></VirtualHost> > configs). Right, we agree on this. Your proposal and mine are almost equivalent semantically, and I'm not quibbling over the details of the semantics very much. My only issue is syntax. I want a syntax which explicitly states the hierarchy of the comparisons. I feel explicit syntaxes are easier to understand. I also want a syntax which remotely resembles something we already have; yours doesn't. Even if you changed your syntax to get rid of the colons and use quoted strings (which we already have support for) it still bothers me. I would have to remember what each of the 4 positional parameters mean, even though I'll only ever use two of them, host and IP address, which we can probably agree are the two most commonly used fields. The positional ordering also does not correspond to the hierarchy you're proposing. Dean
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how soon should you meet your lover's girlfriend?
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Location: Upstate New York, USA
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It sounds like many here have a common wish - for everyone to meet in some form or another before it gets too serious.
Where we differ is on the definitions of "meet" and "too serious". For some sex, by definition, only happens when things are serious, for others it's more casual. As long as everyone involved in the relationship is compatible on this, then it's going to work - if they aren't, then it's going to be a problem really quickly.
For others, "meet" means face-to-face, because they get a far better "read" on someone when having a face-to-face interaction then by emails or phone calls, whereas for others the emails and phone calls are perfectly fine. Again, as long as everyone involved is on the same page, then things can be very functional.
Knowing this, I think that it's really important in the very early discussions about a relationship, to find out where everyone stands on these points and come to an agreement. This will involve lots of anguish and people wasting their time on something that was destined to fail.
This has been very interesting, and has helped clarify some stuff for me. Thank you for the (mostly) civil discussion on this.
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Become A Summit Husky!!!
Summit Prep is currently full for the 2016-2017 school year. If space becomes available, we will update this page with a transfer lottery date.
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Kennedy, William
AbeBooks Seller Since March 13, 2004Quantity Available: 1
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AbeBooks Seller Since March 13, 2004Quantity Available: 1
About this Item
Bibliographic Details
Title: Roscoe
Publisher: Viking Penguin, New York, NY, U.S.A.
Publication Date: 2002
Binding: Hard Cover
Book Condition:Fine
Dust Jacket Condition: Fine
Edition: First Edition.
About this title
You've never met a politician like Roscoe (or have you?): a suave Falstaffian in a double-breasted white Palm Beach suit, unscrupulous, brilliant, exploding with courtly romance. It's V-J Day, the war's over, and Roscoe, after twenty-six years as chief braintruster of Albany's notorious political machine, decides to quit politics forever. But there's no exit, only new political wars, mysterious death, self-destructive party feuds, and scandalous threats to his beloved and her family.
Roscoe, the chivalrous warrior, turns his own life, and everybody else's, inside out to cope with the erupting disasters and finds fraudulence an extremely effective combat weapon. "Righteousness doesn't stand a chance against the imagination," he concludes. Every step forward leads Roscoe back to the past-to the early loss of his true love, his own peculiar heroics in the First World War, the takeover of city hall, the fight with FDR and Al Smith to elect a governor, and the methodical assassination of gangster Jack (Legs) Diamond.
Roscoe, William Kennedy's seventh novel in his Albany cycle, illuminates the high and low of Albany life between the world wars. It is an odyssey of great scope and linguistic verve, a deadly comic masterpiece from one of America's most important novelists.
"Kennedy's beguiling yarns are the kind of family myths embellished and retold across a kitchen table at night: whiskified, raunchy, darkly funny, tangles of old resentments and fresh exasperations." (Time )
"When Kennedy writes about Albany, New York, he is in fact holding up a mirror to all of American history . . . his fictional terrain can be compared to the Faulknerian South in its complex richness." (The Washington Post)
"Kennedy's power is such that the reader will follow him almost anywhere, to the edge of tragedy and back again to redemption." (The Wall Street Journal)
Insubstantial but charming, William Kennedy's Roscoe seems to unintentionally resemble many of the politicians it depicts. The seventh novel in Kennedy's Albany series, Roscoe follows Roscoe Conway, a quick-witted, charismatic lawyer-politician who has devoted much of his life to helping his Democratic Party cohorts achieve and maintain political power in 1930s and '40s Albany, New York. It's 1945, and Roscoe has decided to retire from politics, but a series of deaths and scandals forces him to stay and confront his past. Kennedy takes the reader on an intricate, whirlwind tour of (mostly) fictional Albany in the first half of the 20th century. He presents a mythologized, tabloid version of history, leaving no stone unturned: a multitude of gangsters, bookies, thieves, and hookers mingle with politicians, cops, and lawyers. In the middle of it all is Roscoe, the kind of behind-the-scenes, wisecracking, truth-bending man of the people who makes everything happen--or at least it's fun to think so. Kennedy shows an obvious affection for his book's colorful characters and historic Albany, and he describes both with loving specificity. Though the book often works as light comedy, its clichéd plot developments and stereotypical characters undermine its serious concerns with truth, history, and honor. "You've never met a politician like Roscoe Conway," promises the book's jacket blurb. But we have, through his different roles in countless films and TV series. As with its notoriously deceitful hero, Roscoe is likeable as long as you don't take it too seriously. --Ross Doll
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The characteristic functions of various functions of a real or vector random variable are expressed in terms of the characteristic function of that variable. In the examples there is special emphasis on the stable distributions that have real characteristic functions. Some of the results suggest the practicability of generalizing traditional multivariate analysis beyond the multi-Gaussian model.
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Your Own Worst Enemy
Do you ever wonder if you secretly want to fail? It's hard to believe, but some people do want to fail. They may not want to do better than their parents, and secretly, they set themselves up for failure. Others fear success. They associate success with added responsibility, and they want to avoid responsibility at all costs. But if you're like most people, you want to succeed. For whatever reason, however, you find you make trading mistakes that result in losses. There are times when you are your own worst enemy.
There are a number of ways that you may be your own worst enemy, but here are some common ones.
Many novice traders risk money they can't afford to lose, and understandably, feel on edge as they execute and monitor a trade. You can't concentrate very easily when you are agitated. In order to trade calmly and rationally, it is vital to trade only with money you can afford to lose. In addition, it is important to manage risk, so that you minimize the amount you can lose on any single trade. If you truly have nothing to fear by losing, you'll feel more relaxed. You will trade effortlessly, and you won't make as many trading errors.
Don't miss sleep. When you're tired, it's hard to concentrate. When you are worn out or tired, you are understandably ready to lash out, or feel extreme frustration, even when you face a minor setback. Concentration requires energy, and you don't have adequate energy to trade when you are tired. So get the sleep you need to stay alert and ready for action.
Don't trade by the seat of your pants. Many novice traders don't map out their trades in enough detail. They don't decide beforehand where to enter or where to exit. When they actually try to execute the trade, they find it difficult to stick with their trading plan because it is unclear and difficult to follow. They don't know exactly what to do and when. And so, they panic at critical moments of investing. Carefully outlining a plan and following it will help you avoid costly trading errors.
Trading is hard enough. You don't need to sabotage your own efforts. By trading with money you can afford to lose, staying calm and rested, and by following a well-defined trading plan, you can stay at the top of your game and take home profits.
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Fix It/book, Cassette, and Filmstrip
One morning Emma gets up early to watch television. But the TV won't work. No one can fix it-not Mom, not Dad, not even the repairman. Neither can a balloon or a song or playing horsey fix Emma's disappointment. But then Emma's mother reads her a book. "Read it again," says Emma. "And again." And when Emma's father finally realizes what's wrong with the TV-it was just unplugged!-Emma is too busy reading to care. The power of books to enthrall has rarely been presented in such a clever, simple way for the TV-savvy preschool set.
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Grilled Lamb and Red Onion Tacos with Tomato-Mint Salsa
Slideshow: More Amazing Lamb Recipes
- Servings: 8
- 2 tablespoons ancho chile powder
- 1 small onion, coarsely chopped
- 10 garlic cloves, chopped
- 3 chipotle chiles, chopped
- 1 cup fresh orange juice
- 1 cup extra virgin olive oil
- Two lamb tenderloins (2 pounds each)
- Salt and freshly ground pepper
- 2 grilled lamb tenderloins, sliced thinly
- 8 flour tortillas
- 1 cup grated Monterey Jack cheese
- 1 cup grated white cheddar cheese
- 1/2 cup crumbled feta cheese
- 1 large red onion, sliced and grilled
- 1/4 cup fresh mint chiffonade
- Salt and freshly ground pepper
- 1/4 cup olive oil
- Tomato-Mint Salsa, for serving
- 2 medium tomatoes, coarsely chopped
- 2 tablespoons finely diced red onion
- 1 jalapeÑo pepper, seeded and minced
- 3 tablespoons balsamic vinegar
- 1/4 cup fresh mint chiffonade
- 2 teaspoons olive oil
- Salt and freshly ground pepper
How to make this recipe
Make the tacos
Combine all ingredients in a bowl and set aside for 1 hour at room temperature.
Make the tacos
Combine the ancho powder, onion, garlic, chipotles and orange juice in a blender and blend until smooth. Slowly add the olive oil and blend until emulsified. Place the lamb in a shallow baking dish, cover with the marinade and let marinate for 1 hour. Heat the grill. Season the lamb with salt and pepper and grill for 3 minutes on each side for medium-rare. Let sit 10 minutes before slicing thinly.
Make the tacos
For each taco, lay four slices of lamb over 1/2 of the tortilla, sprinkle with 2 tablespoons each of the Jack and cheddar cheeses and 1 tablespoon of the feta cheese. Top with grilled onion and mint. Season to taste with salt and freshly ground pepper. Fold over the tortilla and brush with olive oil. Grill for 3 minutes on each side or until crispy and the cheese has melted. Serve with the Tomato-Mint salsa.


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Last week, I found my personal paradise. A place where all things that inspire me are categorized, labeled with my notes, and the sources are automatically tagged… a place called Pinterest.
If you have no clue what Pinterest is, here's the 411: It's basically a virtual pin board where you "pin" things you find online that inspire you or want to make note of. Things like home decor ideas, recipes, DIY projects you want to try out, entertaining ideas… lolcats, schematics for building your own rocket ship… whatever your heart's desire. The ultra cool part is when you click your Pin bookmark, Pinterest automatically saves the URL so you can easily go back and see more information about the image you pinned! Amazingly awesome.
Another favorite feature is you can follow other people's pins. Think of it as Twitter… but with photos. Overall, Pinterest is a perfect recipe for a new obsession. Yet again, amazingly awesome.
I encourage non-"pinners" to consider jumping on the Pinterest bandwagon simply because it's fun and you'll be suprised how inspirational it is to see all of the things that catch your eye in one place. I've got a feeling this is eventually going to be THE go to for inspiration, project ideas, etc.
Note: if you request an invite it can take up to two weeks but if you have a friend who already uses Pinterest, they can send you an invite for instant pinning.
I just started "pinning", so my categories (or "boards" as they call them) are a little on the sparse side, but if you would like to get a taste of what Pinterest is all about, or if you already have a Pinterest account, you can find my page right here: http://pinterest.com/7thhouse.


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Is the Oil & Gas industry ready to drive the next wave of efficiencies?
...with the EcoStruxure™ Facility Expert (formerly known as Facility Hero) app in hand! Discover how you can optimize your maintenance efforts today.
A single, open IoT system that lets you compete in today's digital world, EcoStruxure™ is a game-changer.


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With its pleasant crunch and natural sweetness, fresh coconut adds texture and flavor to a healthy diet. While it's high in calories -- a 2-inch-by-2-inch piece has about 160 calories -- coconut meat also has lots of nutritional value, and it's an especially rich source of healthy fats and beneficial minerals.
Potentially Heart-Healthy Fats
Most of coconut meat's calories come from fat. The 15 grams of total fat in a serving of coconut account for about 85 percent of its total calories. And while most of that fat -- a little over 13 grams -- is saturated, which isn't normally good for your heart, the saturated fatty acids in coconut actually benefit your cholesterol levels. The fats in coconut meat increase high-density lipoprotein, or HDL -- a form of cholesterol that benefits your cardiovascular health. It's still not known whether eating coconut meat actually reduces your risk of heart disease, however.
Beneficial Fiber
Coconut meat also offers cardiovascular benefits because it's high in fiber -- each serving of fresh coconut meat offers 4 grams, which is 16 percent of the daily value. Foods high in fiber can help lower your cholesterol levels because fiber can bind to cholesterol in your digestive tract and flush it out of your system. Fiber might also help with weight control -- it's not a source of calories like other carbohydrates but still helps you feel full -- and promotes digestive health by fighting constipation.
Beneficial Minerals
Coconut meat also serves as a source of minerals. It's particularly high in essential manganese and copper, which activate antioxidant enzymes needed to protect your cells from damage. Copper's also involved in making elastin and collagen, two proteins that strengthen your tissues, and copper also plays a role in energy production. Manganese promotes healthy bone development, and it helps you process carbohydrates and proteins. A serving of raw coconut meat provides 10 percent of the DV for copper and 34 percent for manganese.
Eating More Coconut Meat
Enjoy coconut meat on its own as a high-energy snack -- just chop into bite-size pieces and go. The meat's subtle flavor also works well as an unexpected addition to fruit salads -- it's especially good paired with mixed berries or with chopped pineapple and papaya. If you're feeling more adventurous, try blending chopped coconut meat together with frozen pureed acai, a few handfuls of baby spinach and a splash of unsweetened almond milk for a tropical-flavored, nutrient-dense smoothie.
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text:
Sediment transport in oscillatory boundary layers is a process that drives coastal geomorphological change. Most formulae for bed–load transport in nearshore regions subsume the smallest–scale physics of the phenomena by parametrizing interactions amongst particles. In contrast, we directly simulate granular physics in the wave–bottom boundary layer using a discrete–element model comprised of a three–dimensional particle phase coupled to a one–dimensional fluid phase via Newton's third law through forces of buoyancy, drag and added mass. The particulate sediment phase is modelled using discrete particles formed to approximate natural grains by overlapping two spheres. Both the size of each sphere and the degree of overlap can be varied for these composite particles to generate a range of non–spherical grains. Simulations of particles having a range of shapes showed that the critical angle—the angle at which a grain pile will fail when tilted slowly from rest—increases from approximately 26° for spherical particles to nearly 39° for highly non–spherical composite particles having a dumbbell shape. Simulations of oscillatory sheet flow were conducted using composite particles with an angle of repose of approximately 33° and a Corey shape factor greater than about 0.8, similar to the properties of beach sand. The results from the sheet–flow simulations with composite particles agreed more closely with laboratory measurements than similar simulations conducted using spherical particles. The findings suggest that particle shape may be an important factor for determining bed–load flux, particularly for larger bed slopes.
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A mother whose first-grade daughter died at school from an allergic reaction to a peanut can sue the school system for allegedly defaming the mother by trying to shift blame to her for the child's death, a unanimous Virginia Supreme ...Read More »
Court hears judge-or-jury 'bad faith' case
A former legislator and a constitutional scholar squared off June 2 in the Supreme Court of Virginia on whether a judge or jury can decide an insurer bad faith claim under Virginia Code § 38.2-209. It wasn't just the lawyers for the parties ...Read More »
Justices deny rehearing in major product liability case
The Supreme Court of Virginia has denied rehearing of its decision to overturn a $14.14 million verdict for a Pulaski County man who blamed an alleged air bag defect for his car crash injuries. On Jan. 8, the court ruled ...Read More »
Court allows condemnation jury to hear early appraisal
A unanimous Supreme Court of Virginia says the state highway department cannot block evidence of an early, high-dollar appraisal when it asks a jury to put a value on land taken for a road project. The court rejected the state's ...Read More »
Virginia court can't enforce Yelp subpoena
A Virginia carpet-cleaning business could not go through a Virginia circuit court to compel Yelp to identify "John Doe" posters who allegedly defamed the business on the consumer review website, the Supreme Court of Virginia has ruled. The court as ...Read More »
Defendant wins 'prevailing party' fees in split decision
A defendant who won a suit alleging breach of a shareholder agreement was entitled to attorney's fees under a provision that allowed fees to a party who hired a lawyer "to enforce" the agreement, the Supreme Court of Virginia said ...Read More »
Lawyer survives possible murder threat, avoids liability
A Harrisonburg lawyer can breathe a second sigh of relief after the Supreme Court of Virginia last month found no basis to hold him liable for the 2011 murder of a process server by an angry husband. The lawyer himself ...
Tagged with: Judge Jane Marum Roush Justice S. Bernard Goodwyn Supreme Court of Virginia NewsRead More »
'Sufficiency' challenge was sufficient
When Bradley Mowbray appealed his misdemeanor assault and battery and property destruction convictions, he simply asserted the trial judge "erred in finding the defendant guilty of a violation of Va. Code §§ 18.2-57 and 18.2-137." The clerk of the Court ...Read More »
SCOP badge claim no bar to conviction
The prosecution did not have to show that a special conservator of the peace was wearing a badge to prove he was acting as a "law enforcement officer" when patrolling for a private security firm, the Supreme Court of Virginia ...Read More »
'Good cause' to hear anti-sodomy cases
In Toghill v. Commonwealth, the Supreme Court of Virginia found good cause not to apply Rule 5:25, Virginia's contemporaneous objection rule, and went on to hold that Virginia's anti-sodomy law is constitutional, as applied to a defendant who solicited oral ...Read More »


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Over the axle trailers?
Discussion in 'Tow & Trailer' started by Fubeca, Dec 11, 2003.


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Watch Mitt Romney Iron Himself in the Trailer for 'Mitt'
There are a lot of fun things to be found in the trailer for Mitt, but the unequivocal highlight is watching Mitt Romney iron his suit while it's still on.
Premiering Jan. 24 on Netflix, Mitt follows Romney and his family through his two failed presidential bids, from 2006 to 2012. … More »


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Pleasant and helpfull staff. amid construction/refurbinsing at this time. new lighting and carperts were wonderfull. Closet could be designed better and clothing iron should be replaced, but still functional. Breakfast is basic, hot, and adequate. Need better quality bacon.
- Official Description (provided by the hotel):
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I would like to see proof. After all, we all know that the national government can lie about weapons of mass destruction - could these be Sadam's weapons, finally being used after transport out of Iraq as was reported at the time of the invasion or just before it began?
The answer is that under the Obama Administration, it does not matter where they came from and they will be the pretext for supplying arms to the Muslim Brotherhood all for the stated purpose of the "democracy movements" throughout all the Mideast.
No one cares where they came from. They only care how we will react as a people on hearing the scary news.
After all, we will invade for the safety of the children...
This is probably a lie. Our government now lies to us constantly, and about almost everything. Nearly nothing they say is honest. And now, this latest propaganda is offered.
I'm waiting for stories of Syrian troops unplugging incubators and catching Belgian babies on their bayonets.
Hillary and Obama come out and announce "if chemical weapons are used, we will intervene".
But what they actually did was precisely tell the moslem brotherhood wahabbi rebels, "we will intevene on your behalf as soon as you can provode "proof" of a chemical attack."
This can be done with a few Libyan shells provided by their like minded cohorts there, or by the CIA. Then there will be a "limited intervention to secure the gas weapons".
This of course, means you must wipe out the Syrian Air Force. You must wipe out all the Syrian Anti-Air capacity to permit a permissive air environment.
You must secure a large airport and possibly a port and a supporting MSR.
The force seizing the gas supplies will report that they are in awful condition and it will be a slow to remove them safely, so we will need to seize a large base to have a place to deal with their storage and removal.
This will mean all Syrian ground forces which can threaten that base must be wiped out.
As we do attack the Syrian Air Force, AAA, etc, we will probably directly endanger or kill some Russians.
This boy-king Obama is going to get us into a war with Russia before he is done.
And all for what? To help the same crew that took over Egypt grab Syria.


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Melissa Robinson, who co-owns the Sears location in Summerside with her husband Mark, stands at the customer service desk inside the half-empty store. The store, due to a decision by Sears Canada, will be closing its doors for good on Jan. 2, leaving 10 people out of work.
©Nancy MacPhee/Journal Pioneer
Telling her employees that they soon would be out of work is one of the hardest things Melissa Robinson has ever had to do.
Robinson and husband, Mark, own the Sears store in Summerside.
Recently, the couple was told by Sears Canada's corporate office that it was closing, early in the new year, the store they've owned and operated since 2009, putting 10 people out of work.
"We are really a family here. It is very hard at Christmas," said Robinson, choking back tears. "To have to tell your employees that we don't have employment for them at Christmastime is one of the worst things we could ever imagine."
It was a devastating blow, a decision, Robinson was quick to point out, wasn't that of the couple, and one, she added, that came as a shock.
"They are choosing not to be in Summerside at this point."
The store, located at 645 Water St., includes a furniture and appliance showroom and a catalogue outlet.
The location services those from Hunter River all the way to the western tip of P.E.I.
"Our customers are what we are going to miss the most," said Robinson. "We have a very loyal customer base that we appreciated over the last six years. They have supported a young couple and a family business that we have been growing. They have been very good to us and they will definitely be missed.
"We hope we can find a way to service them in the future, in some way or capacity."
She wouldn't say exactly when she and her husband found out news of the closure.
"It was definitely rather short notice," added Robinson. "I am not at liberty to discuss the dates. We gave the staff notice as soon as we had confirmation of firm dates of when it was going to happen."
She referred that question and others specifically related to the closure of the store to Vincent Power, vice-president of corporation communications with Sears Canada.
Calls made to Power on Tuesday have not yet been returned.
For now, and until Jan. 2, it will be business as usual, said Robinson.
Customers can still pick up and return parcels at the Summerside location.
There is also a liquidation sale of all stock in the store.
"Sears' plan is to sell everything off. All the furniture is being sold at cost and any of the other departments the items are being sold at bottom-dollar prices," said Robinson.
She added that any customers with questions should feel free to call the store.
"Our thoughts go out to customers that are going to have holiday returns and will have to travel to Charlottetown to do that."


text:
Open Shop: Introduction to Python
Parts and Crafts
Thursday, January 10, 2013 at 3:00 PM - Thursday, February 7, 2013 at 5:00 PM (EST)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
A five-session introduction to Python, a powerful, popular, and highly alliterative programming language that focuses on clear syntax and easy readability. We'll cover some of the rules and tools and use them to build simple games. Experience is helpful, but not necessary. participants must know how to read and type at a reasonable speed.
Dates: Tuesdays, January 10 – February 7 (5 sessions), 3 – 5 p.m.
Cost: $100, sliding scale ($10-20 / session)
Age: Open to kids of all ages -- some programming experience is useful, must be able to read and type fluently
When & Where
339 Summer St
Thursday, January 10, 2013 at 3:00 PM - Thursday, February 7, 2013 at 5:00 PM (EST)
Add to my calendar
Parts and Crafts


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Mixed Media Southwest Abstract Art Painting Navajo Trail by Colorado
painting by artist Carol Nelson
7 in X 24 in (17.8 cm X 61 cm)
Mixed Media
This painting is available directly from the artist.
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I was in search for a MIDI footswitch, to control my -guitar- effects. Price was high and the functionality not what I was looking for. Next step was to design a simple MIDI Controoler! And ..voila! Source Code/etc are on GitHub. Facebook page (with photos) is here.
MiFaSol – Midi Controller
by Antonis/ /


text:
Master in Engineering Mechanics
The field of Engineering Mechanics includes all areas of the industrial design process that are based on advanced mechanical analysis of product behavior and functionality. The Master's programme equips students with the knowledge and skills required to focus on reliable and sustainable design by simulations and experiments.
Programme outline
The education within the three tracks – Fluid Mechanics, Solid Mechanics and Sound & Vibration – is conducted with involvement from industry and influenced by the latest research. Only one third of the courses are mandatory, so students can tailor the course structure to meet their individual aims. This is very useful for those interested in exchange studies. The theoretical courses are mixed with more applied project courses and the final degree project is often performed at a company in Sweden or abroad.
Degree project
The fourth semester is spent working on a five month degree project, where the student has the opportunity to investigate a specific area in-depth. The degree project is performed either in industry or at a university department, in Sweden or abroad. Students doing their degree project close to KTH may also take courses in parallel with their thesis work.
Career prospects
Students who graduate from the Engineering Mechanics programme will be proficient in fluid mechanics, solid mechanics and sound & vibration – areas that are tied to a multitude of industries. Graduates will find a wide range of job opportunities in research and development within industrial companies, consulting firms and research institutes, not only in mechanical engineering, but also in other engineering fields.
This school offers programs in:
- English
Last updated March 23, 2017


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Distances are calculated in a straight line and may not reflect actual travel distance.
It is important to note that this mortgage calculator is for guidance purposes only. Mortgage lenders have their own affordability criteria therefore it is important you speak to one of our Mortgage Advisers for further information and a more accurate indication of what products may be available to you and what your monthly mortgage payments are likely to be.
The main entrance is via the first floor which has a welcoming entrance hallway with stairs up to the second floor and down to the ground floor.
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Everybody unwinds from a stressful day differently—some people get a massage, whereas others watch flames engulf a big pile of tires. Let tension melt away with this Groupon.
Choose Between Two Options
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Customers pick from a Swedish, deep-tissue, or pregnancy massage. This Groupon is redeemable at Hillary's Providence location throughout the week and at Glow Face & Body Spa in Warren on Wednesdays.
Hillary Adams Massage
In high school, Hillary Adams stumbled upon a PBS TV special about massage therapy that inspired her to experiment with massage techniques on her friends and family. After graduating from college with degrees in English literature and anthropology, she reignited her teenage passion by enrolling at Bancroft School for Massage Therapy. Since completing massage school in 1999, Hillary has become a nationally certified and Rhode Island–licensed massage therapist who uses 100% natural oils and various modalities to treat clients ranging from pain-stricken weekend warriors to an 11-year-old girl receiving her first-ever massage.
Located inside a century-old Victorian home next to the historic Ladd Observatory, Hillary's Providence office reverberates with soothing new-age tunes by Dean Evenson. She also works out of Glow Face & Body Spa in Warren on Wednesdays, which houses a massage room furnished with modern decor and artwork.


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In what may be viewed as the final scene-setter before their Southeastern Conference Western Division showdown, the BCS standings, as expected, retained LSU and Alabama as the top two teams in college football's most important rankings.
LSU (8-0, 5-0 SEC) and Alabama (8-0, 5-0) both enjoyed off weeks as they prepare to play at 7 p.m. Saturday in Tuscaloosa, Ala. The game will be televised by CBS, which last week moved the game from an afternoon kickoff to a prime-time slot.
The winner, as ESPN's Rece Davis said Sunday night as the latest BCS standings were unveiled, "will have a clear path to the 2012 All-State BCS national championship game" on Jan. 9 in the Mercedes-Benz Superdome.
LSU sits atop The Associated Press, the USA Today and the Harris Interactive polls. Alabama trails the Tigers in all three. The AP poll, however, is not a component of the BCS standings.
The BCS standings use the Harris poll, the coaches' poll and computer ratings to determine which teams play for the national championship.
In those BCS standings, LSU leads at .9734, with Alabama second at .9591. Oklahoma State is third at .9310, while Stanford is fourth at .8615.
Stanford (8-0) moved up two places to fourth after beating Southern California 56-48 in triple overtime Saturday night. It was the Cardinal's first win of the season against a ranked team.
Boise State (7-0) slipped a spot to fifth, while Clemson (7-1) fell from fifth to 11th after its first loss.
Oklahoma State, if it wins out, seems to be in position to reach the title game. The computer ratings have the Cowboys, who host Kansas State on Saturday and Oklahoma on Dec. 3, No. 1.
LSU and Alabama are tied for the second-best computer rating.
Oklahoma State is No. 3 in the Harris poll and No. 4 in the coaches' poll, and Stanford is No. 4 and No. 3. But the difference between the Cowboys (.9310 BCS average) and the Cardinal (.8615) is the computer ratings.
While there are plenty of teams jockeying behind LSU and Alabama, the showdown Saturday will establish the winner as the favorite to reach the BCS title game.
"Of all the one versus twos I've seen, this one is the most anticipated, at least in my lifetime," ESPN's Kirk Herbstreit said. "I cannot wait to see these two teams get together."
The loser of the showdown could face a tricky climb to get back into the top two of the BCS standings.
Of the loser, Brad Edwards, ESPN's BCS analyst, said, "I think that team will still fall behind the major undefeateds, regardless of what voters think as far as what teams are the two best teams in college football … and then would have to work its way up."
After Saturday, LSU has two SEC games remaining: Nov. 19 at Ole Miss and Nov. 25 against No. 8 Arkansas.
Alabama, meanwhile, will have SEC games left Nov. 12 at Mississippi State and Nov. 26 at No. 25 Auburn, the defending national champion.
Of LSU, Herbstreit said, "That team has the 'it' factor. They've had adversity. They've played on the road. … They bring swagger and they bring confidence when they show up Saturday in Tuscaloosa."
Stanford is sixth in the computers because of a weak early season schedule. The Cardinal should be getting a boost from here on out.
After a road game Saturday against Pac-12 rival Oregon State, the Cardinal play Oregon, eighth in the BCS standings, at home. They finish with California and Notre Dame at home and could play in the Pac-12 championship, too — probably against Arizona State.
It might not be enough to catch Oklahoma State. The Cowboys face Big 12 rival Kansas State, 14th in the BCS standings, on Saturday, have road games against Texas Tech and Iowa State, and finish with a home game against Oklahoma.
The Big 12 no longer has a championship game.
For Boise State, the formula is the same as it has always been. The Broncos' conference schedule — this year in the Mountain West — presents few stiff challenges, so Boise State probably needs to go undefeated just to play in one of the four marquee bowl games. The Broncos have done that twice. To reach the national championship game for the first time, Boise State will need the teams ahead of it to lose.
Wing-ing it
Going into Saturday's play, LSU freshman punter Brad Wing ranked third in the SEC with a 44.4-yard per punt average behind only Georgia's Drew Butler (46.1 ypp) and Arkansas' Dylan Breeding (44.7).
According to the SEC, Wing has the top average among freshman punters in the country. Wing has helped LSU lead the SEC and rank sixth nationally with a net punting average of 41.1 yards per punt.
The numbers game
A look at some key numbers going into Saturday's showdown:
1 — The number of times LSU and Alabama have each turned the ball over in SEC play this season (both interceptions).
2.38 and 0.88 — Number of sacks and sacks allowed by LSU per game this season, second and first, respectively, in the SEC.
3 and 7 — Number of points allowed by LSU and Alabama, respectively, off turnovers. LSU has committed three turnovers, Alabama eight. The SEC record for fewest points allowed off turnovers is 14 by Alabama in 2005.
7 — The total number of punt return yards allowed by LSU this season. The next best team going into Saturday's play is Auburn with 41.
10-4 — LSU's record in games at Alabama (Tuscaloosa and Birmingham) since 1982.
39 — Number of times the road team has won in this series out of 74 total meetings.
66.7 — Alabama's percentage for red zone defense (five TDs, one field goal in nine attempts), which leads the SEC.
93.8 — LSU's percentage for red zone defense, which is last in the SEC. Teams have scored 15 of 16 times against the Tigers (nine touchdowns, six field goals).
97.4 — LSU's percentage for red zone scoring, which is tops in the SEC and second nationally behind only Stanford. The Tigers have scored 38 of 39 times in the red zone this season (31 touchdowns, seven field goals). The only time LSU failed to score was at West Virginia, when Rueben Randle dropped a pass in the end zone and Drew Alleman followed by missing a 30-yard field goal attempt. Alabama ranks 38th nationally at 85.4 percent (24 TDs and 11 field goals in 41 attempts).
Advocate Assistant Sports Editor Joseph Schiefelbein, Advocate sportswriter Scott Rabalais and The Associated Press contributed to this report.


text:
Beyond the Edge
Edmund Hillary's 1953 ascent of Mt. Everest was an event that amazed the world and led to a new age of mountaineering and ambitious exploration. Hilary's triumphant saga is retold using both modern footage, and recordings and photos from the time.
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Movie Details
- Director
- Leanne Pooley
- This movie is
- Inspiring
- Format
- Ratings
NRNot rated. This movie has not been rated by the MPAA.
NoClosed captioned
Beyond the EdgeClose
For Parents
Common Sense Note
- Age appropriate
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- Not appropriate for kids of the age most likely to want to see it


text:
Changes in the transcriptome of the malaria parasite
Eukaryote microbial genomics
The transmission of the malaria parasite
To better understand gene regulations during the initial phase of malaria parasite transmission, we focused on the transcriptome changes that occur within the first half hour of parasite development in the mosquito. Comparison of mRNA levels of
For a subset of 27 genes, transcript changes during gametogenesis were studied in detail by real-time RT-PCR. Of these, 22 genes were expressed in gametocytes, and for 15 genes transcript expression in gametocytes was increased compared to asexual blood stage parasites. Transcript levels of seven genes were particularly high in activated gametocytes, pointing at functions downstream of gametocyte transmission to the mosquito. For selected genes, a regulated expression during gametogenesis was confirmed on the protein level, using quantitative confocal microscopy.
The obtained transcriptome data demonstrate the regulations of gene expression immediately following malaria parasite transmission to the mosquito. Our findings support the identification of proteins important for sexual reproduction and further development of the mosquito midgut stages and provide insights into the genetic basis of the rapid adaption of
Up to date, the tropical disease malaria is one of the most devastating infectious diseases in the world, causing 216 million new cases and approximately 655.000 casualties each year
The transmission of malaria parasites from the human to the mosquito is mediated by specialized sexual precursor cells, the intraerythrocytic gametocytes. Maturation of
When entering the mosquito midgut together with the blood meal, the gametocytes become activated from the dormant stage by external stimuli, i.e. a drop in temperature and the contact with the mosquito-derived molecule xanthurenic acid (XA) (reviewed in
During gametocytogenesis,
Gametocyte maturation and gametogenesis are particularly accompanied by the coordinated expression of numerous adhesive surface proteins, including the EGF domain-containing proteins Pfs25 and Pfs28, the 6-cys proteins Pfs230 and Pfs48/45, and the LCCL domain-containing PfCCp proteins. It is noteworthy that the majority of these proteins can be divided into two classes: One class of the adhesion proteins, including Pfs230, Pfs48/45 and the six PfCCp proteins, is expressed within the parasitophorous vacuole (PV) of the developing gametocyte, and the majority of these proteins assemble to multimeric protein complexes
While astonishing advances have been made in previous years in unveiling the molecular basis of parasite transmission from the mammalian host to the mosquito, the currently available data are fragmented and major key players for gametogenesis and adaptation have not yet been identified. We thus aimed at investigating the changes in transcript levels during gametocyte activation in order to gain in-depth knowledge on the molecular switch-over that takes place in the parasite during transmission. We selected the suppression subtractive hybridization (SSH) technique to experimentally screen for genes differentially expressed during gametocyte activation, because this PCR-based method amplifies induced genes while simultaneously suppressing house-keeping genes, and is thus particularly suited to discover new or unexpected genes
Results and discussion
To determine changes in the transcriptome of malaria parasites during the initial phase of transmission to the mosquito, we conducted a SSH on mRNA isolated from mature non-activated gametocytes and from gametocytes at 30 min after in vitro activation, which have completed gametogenesis
We identified a total number of 126 genes, for which expression levels changed in the gametocytes during activation. The majority of genes can be assigned to six major ontology groups (Figure
Figure 1
Ontology groups of
Ontology groups of
Roughly 60% (76/126) of the SSH genes have been detected in the currently available
We used real-time RT-PCR analyses to confirm the transcript changes in gametocytes during activation for a subset of the identified genes and to determine, if these genes become up- or down-regulated in their expression during gametocyte activation. Total RNA was isolated from immature (stages III and IV) and mature (stage V) gametocytes of
Figure 2
Changes in transcript expression levels during the formation and activation of
Changes in transcript expression levels during the formation and activation of
We chose 27 genes from the SSH analysis for comparison of transcript abundance, with representatives from all ontology groups. The gene
Transcript expression levels were measured via real-time RT-PCR and calculated by the 2-ΔCt method
Seven genes were identified, i.e. PF3D7_0827800 (
Actin I was described as part of the plasmodial motor complex (reviewed in
Eight genes had an increased transcript expression in gametocytes compared to asexual blood stage parasites, and transcript levels either remained constant or decreased following activation. These include PF3D7_0302100 (
Pfs16 is a transmembrane protein of the gametocyte PVM
The presenilin-like signal peptide peptidase (SPP) was previously reported to be involved in merozoite invasion and cleavage of the erythrocyte cytoskeletal protein band 3
The gene product of PF3D7_0817600 comprises a MAC/PF domain, similar to the MAC/PF domains of the previously described plasmodial perforin-like proteins PPLP1-5
Three genes were predominantly expressed in the asexual blood stages and show a resurgence in transcript expression in gametocytes during maturation, i.e. PF3D7_1238900 (
For four genes, PF3D7_0818900 (
The genome of
The transmembrane protein GAP50 is part of the IMC of the parasite invasive stages, including the ookinete
FBPA is an enzyme of glycolysis, and in
Transcript expression for five genes, PF3D7_0825800 (
The early transcribed membrane proteins (ETRAMPs) are proteins of the plasmodial PVM
For selected genes we subsequently investigated the expression changes on the protein level. Antibodies against the proteins PK2, CLK-4, actin I and actin II, GAP50, proteasome SU α5, Pfs16, Pfs25 and PPLP6 were used to immunolabel the respective proteins in samples of
Figure 3
Changes in protein expression during formation and activation of
Changes in protein expression during formation and activation of
Interestingly, we demonstrated a slight up-regulation in the protein expression of GAP50 at 30 min p.a., although a decrease in transcript levels was described during this process. This phenomenon can be explained by the fact that translationally repressed
In summary we demonstrate that the majority of genes, which are regulated in their expression levels during gametocyte activation, have functions assigned to signaling, cell cycle and gene regulation, and proteostasis, or that they are cytoskeletal or cell surface proteins. The regulated expression of proteins involved in signaling and cell cycle control is on the one hand important for the signaling pathways that result in the induction of gametogenesis, once temperature drop and XA are perceived by the parasite
Furthermore, induction of gametogenesis following gametocyte activation results on the one hand in the destruction of the gametocyte IMC and relocation of cytoskeletal proteins
Noteworthy, the SSH analysis identified a number of unknown proteins with transmembrane domains and/or signal peptides, and for six of them, high transcript levels were shown in mature and activated gametocytes. For three of the genes, expression in the gametocytes was higher as compared to asexual blood stage parasites, and these genes might encode for proteins important for gametocyte development or gametogenesis.
The here presented data on the changes in the
The following antibodies were used in this study: mouse antisera against Pfs16 and Pfs230 (kindly provided by Kim Williamson, Loyola University Chicago), proteasome SU α5
Parasite culture
Gametocyte-less F12 strain and gametocyte-forming NF54 isolate of
Construction of a subtracted cDNA library using the SSH method
Total RNA was isolated from Percoll-enriched stage V gametocytes before and at 30 min p.a., using Trizol reagent (Invitrogen, Karlsruhe, Germany). Subsequently, mRNA was isolated from total RNA using the Oligotex mRNA Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions, and the SSH analysis was conducted as previously described
Colony PCR, sequencing and computer analysis of cDNA sequence data
A preliminary colony PCR screen on 20 colonies was performed with vector-specific primers, i.e. T7-promotor: 5′- TAATACGACTCACTATAGGG-3′ and SP6: 5′-ATTTAGGTGACACTATAG- 3′ (purchased from Thermo electron, Waltham, MA, USA), using the following conditions: denaturation at 95°C for 5 min followed by 30 cycles of denaturation at 95°C for 15 s, annealing at 43°C for 20 s, and extension at 72°C for 60 s. A final 7-min 72°C step was added to allow complete extension of the products. The colony PCR showed that 75% of clones contained an insert in the vector. Subsequently, 288 randomly picked clones were screened for the presence of the vector. Plasmid isolation of 141 positively screened colonies was performed with the Fast Plasmid Mini kit (Eppendorf, Hamburg, Germany) and the inserts were sequenced (GATC Biotech, Konstanz, Germany; sequences listed in the Additional file
Additional file 1
Colony sequences of SSH analysis.
Click here for file
RNA isolation and real-time RT-PCR
Total RNA was isolated from the mixed asexual F12 cultures, enriched immature NF54 gametocytes (stage III and IV), non- activated mature gametocytes and gametocytes at 30 min p.a. using the Trizol reagent (Invitrogen) according to the manufacturer's protocol. RNA preparations were treated with RNAse-free DNAse I (Qiagen) to remove gDNA contamination, followed by phenol/chloroform extraction and ethanol precipitation. All RNA samples had A260/A280 ratios higher than 2.1. Two μg of each total RNA sample were used for cDNA synthesis using the SuperScript III First-Strand Synthesis System (Invitrogen), following the manufacturer's instructions. Controls without reverse transcriptase were used to investigate potential gDNA contamination by diagnostic PCR using
Additional file 2
Sequences of primers used in this study.
Click here for file
Primers for real-time RT-PCR were designed using the Primer 3 software (
Real-time RT-PCR measurements were performed using the Bio-Rad CFX96 Real-Time Detection System. Reactions were prepared in triplicate in a total volume of 20 μl using the maxima SyBR green qPCR master mix (Thermo Scientific, Bonn, Germany) with 20 ng of cDNA and primer concentrations of 0.3 μM. The following PCR cycling conditions were used: an initial denaturation step at 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealing/extension at 60°C for 60 s, followed by a final extension at 95°C for 10 s and a final melt-curve analysis of 55-95°C. Controls without template and without reverse transcriptase were included in all real-time RT-PCR experiments. Transcript expression levels were calculated by the 2-ΔCt method
Recombinant protein expression and production of mouse antisera
Recombinant proteins for CLK4, PK2, and actin I were expressed as fusion proteins with a GST-tag using the pGEX 4 T-1 vector (Amersham Biosciences, Freiburg, Germany), PPLP6 recombinant proteins were expressed as maltose binding protein-tagged fusion proteins using vector pIH902 (kindly provided by K. Williamson, Chicago;
Indirect immunofluorescence assay and signal strength quantification
Asexual F12 blood stage parasites, non-activated NF54 gametocytes and gametocytes at 30 min p.a. were air dried on glass slides and fixed for 10 min in −80°C methanol. For membrane permeabilization and blocking of non-specific binding, fixed cells were incubated for 30 min in 0.01% saponin/0.5% BSA/PBS and 1% neutral goat serum (Sigma-Aldrich) in PBS. Preparations were then successively incubated for 1.5 h each at 37°C with the primary antibody diluted in 0.01% saponin/0.5% BSA/PBS. Binding of primary antibody was visualized using fluorescence-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Alexa Fluor 488 or Fluor 596; Molecular Probes, Karlsruhe, Germany) diluted in 0.01% saponin/0.5% BSA/PBS. Nuclei were highlighted by incubating the specimens with Hoechst nuclear stain 33342 (Molecular Probes) for 1 min. Cells were mounted with anti-fading solution AF2 (Citifluor Ltd., Leicester, UK) and sealed with nail varnish. Digital images were taken using a Leica TCS SP5 confocal laser scanning microscope and processed using Adobe Photoshop CS software. For quantitative evaluation of the average signal intensity, digital images of 20 randomly selected mature and activated gametocytes were taken using a Zeiss LSM 510 confocal laser scanning microscope under the same optimal laser scanning microscopy settings for each antibody. The average fluorescence signal intensity of each cell, as indicated by a line edging the respective cell, was measured by LSM 510 image software and recorded.
Competing interests
The authors of this manuscript declare no competing interests.
Authors' contribution
CJN and MS conducted the experiments. SK, TB, MNA and CW generated antibodies. RF, AV and GP designed the experiments. CJN, MS, GRM, JW, and GP contributed with data analyses. CJN and GP contributed with writing the manuscript. All authors read and approved the manuscript.
We thank Meike Lietzow and Ludmilla Sologub for technical assistance. This study was supported by the Emmy Noether program of the Deutsche Forschungsgemeinschaft and the MALSIG consortium of the EU 7th framework program (GP), by an FCT grant PTDC/BIA-BCM/105610/2008 (GRM), as well as by a grant from the Hessian Ministry of Science and Art via the LOEWE-research focus "Insect Biotechnology" (AV). CJN received a fellowship by the Graduate School of Life Sciences of the University of Würzburg.
Malaria: progress, perils, and prospects for eradication
The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitation
Proteins of the malaria parasite sexual stages: expression, function and potential for transmission blocking strategies
The coming-out of malaria gametocytes
Malaria proteases mediate inside-out egress of gametocytes from red blood cells following parasite transmission to the mosquito
Molecular mechanisms of host cell egress by malaria parasites
A proteomic view of the
Analysis of the
Discovery of gene function by expression profiling of the malaria parasite life cycle
Genomewide identification of genes upregulated at the onset of gametocytogenesis in
Protein export marks the early phase of gametocytogenesis of the human malaria parasite
Proteomic analysis of
Sporogonic development of cultured
PfCCp proteins of
Sexual stage adhesion proteins form multi-protein complexes in the malaria parasite
Family members stick together: multi-protein complexes of malaria parasites
Regulation of sexual development of
Universal features of post-transcriptional gene regulation are critical for
Comparative analysis of septic injury-inducible genes in phylogenetically distant model organisms of regeneration and stem cell research, the planarian
Proteome analysis of separated male and female gametocytes reveals novel sex-specific
Integral membrane protein located in the apical complex of
Differential localization of full-length and processed forms of PF83/AMA-1 an apical membrane antigen of
A multidomain adhesion protein family expressed in
Protein targeting to the parasitophorous vacuole membrane of
Target antigens of transmission blocking immunity on gametes of
Sequential expression of antigens on sexual stages of
Characterisation of
The 230-kDa gamete surface protein of
Critical role for a stage-specific actin in male exflagellation of the malaria parasite
Malaria parasites form filamentous cell-to-cell connections during reproduction in the mosquito midgut
The proteasome of malaria parasites: a multi-stage drug target for chemotherapeutic intervention?
Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method
Genes within the serotonergic system are differentially expressed in human brain
Selective upregulation of a single distinctly structured
Identification of a major
Set regulation in asexual and sexual
Histone lysine methyltransferases and demethylases in
Host-cell invasion by malaria parasites: insights from
Malaria parasites co-opt human factor H to prevent complement-mediated lysis in the mosquito midgut
Reverse genetics screen identifies six proteins important for malaria development in the mosquito
PfSRPK1, a novel splicing-related kinase from
Two nucleus-localized CDK-like kinases with crucial roles for malaria parasite erythrocytic replication are involved in phosphorylation of splicing factor
The systematic functional analysis of
Subcellular localisation of Pfs16, a
Cellular location and temporal expression of the
Alpha-tubulin II is a male-specific protein in
Isotype expression, post-translational modification and stage-dependent production of tubulins in erythrocytic
Promiscuous expression of α-tubulin II in maturing male and female
A Presenilin-like protease associated with
Malaria parasite signal peptide peptidase is an ER-resident protease required for growth but not for invasion
A small molecule inhibitor of signal peptide peptidase inhibits
A member of a conserved
Surface antigens of Plasmodium falciparum gametocytes–a new class of transmission-blocking vaccine targets?
Protein kinases of the human malaria parasite
Characterization of
Versatile TPR domains accommodate different modes of target protein recognition and function
Systems analysis of chaperone networks in the malaria parasite
Two major phosphoproteins of
Proteases and chaperones are the most abundant proteins in the parasitophorous vacuole of
Dissecting apicoplast targeting in the malaria parasite
15-deoxyspergualin primarily targets the trafficking of apicoplast proteins in
Hsp90 as a capacitor formorphological evolution
Post-embryonic functions of HSP90 in
A conserved molecular motor drives cell invasion and gliding motility across malaria life cycle stages and other apicomplexan parasites
Origin, composition, organization and function of the inner membrane complex of
Evolution and architecture of the inner membrane complex in asexual and sexual stages of the malaria parasite
Characterization of an aldolase-binding site in the Wiskott-Aldrich syndrome protein
Metabolic maps and functions of the
WD40 proteins propel cellular networks
Human malaria parasites display a receptor for activated C kinase ortholog
New insights into myosin evolution and classification
Alveolins, a new family of cortical proteins that define the protist infrakingdom Alveolata
Etramps, a new
A systematic analysis of the early transcribed membrane protein family throughout the life cycle of
Organization of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites
A subset of
Falcipains and other cysteine proteases of malaria parasites
Rapid membrane disruption by a perforin-like protein facilitates parasite exit from host cells
A perforin-like protein mediates disruption of the erythrocyte membrane during egress of
Protein kinases of malaria parasites: an update
DNA synthesis in
Plasmodium falciparum erythrocyte invasion: a conserved myosin associated complex
Recombinant Pfs230, a
Complete in vitro maturation of
Improved synchronous production of
Targeting and sequestration of truncated Pfs230 in an intraerythrocytic compartment during
Analysis of the immune-inducible transcriptome from microbial stress resistant, rat-tailed maggots of the drone fly
PlasmoDB: a functional genomic database for malaria parasites


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Professional Web Design
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Get Rekt
Mike Creuzer submitted a new resource:
#Rekt - Get Rekt
Read more about this resource...
I've just checked out the demo and I can't scroll any page when it's finished loading (I can scroll briefly whilst the page is still loading). I'm on Android 4.4.4 using Chrome for Android.
A bug with one of the systems we're using, we'll fix it in the morning. But this should be fixed on our demo.
Thanks, demo now fixed.
Might sound stupid, but how would I install the widgets? framework manager?
That's correct. We will write a resource up on it.
Ok....I am having an issue. Got the logo centered on UI.X but I can't seem to get it to center on #Rekt.
Depending on the nav style centering won't make sense. Reka default nav style cannot have a centered logo because where would the links go
.We build it intuitively.
Thanks, i'd like to know if it's possible to have the widgets show up only on the rekt theme, not on all themes .
@Mike Creuzer I know it says you can do a custom location for the navigation.....can I get you to help me a little please?
What I would love to do is have the navigation under the logo and both the logo and navigation be centered.
Widgets aren't showing up on rekt after activating them in the widget manager, the "sign up" thing underneath the header doesn't show on the demo page either, main reason why I got this theme..
Also how do you use the homepage?
We will be posting documentation tomorrow or over the weekend. It requires creating a new page and using a template include for the homepage.
Gorgeous theme. Love seeing where Audentio is taking XenForo.
Great theme! I have it installed on my board now, but the mobile bug that @Martok mentioned is still affecting this build. Any idea on when the fix will be issued to customers? Thanks!
We released the fixed version Thursday night, could you provide a link to your board please?
waiting on documentation...
Here is the tutorial on setting up the Rekt index: https://xenforo.com/community/resources/configuring-rekt-homepage.4202/
Font awesome forum icons don't work. No matter what I change them to I still get the default icons.
Separate names with a comma.


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LOL Whites Case
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Award Winner: 89-point Silver Medal, Toast of the Coast Wine Competition 2016
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dramatic monologuenoun [ C ]US also dramatic monolog /drəˌmæt.ɪk ˈmɒn.əl.ɒɡ/ /drəˌmæt̬.ɪk ˈmɑː.nə.lɑːɡ/
Thesaurus: synonyms and related words
Examples from literature
- But the largest, if not the greatest work in the volume must be sought for, not in the romances, properly speaking, nor in the lyrics, but in the dramatic monologues.
- Clive, the most popular in style, and certainly one of the finest poems in the volume, is a dramatic monologue very much akin, in subject, treatment and form, to the narratives in the first series.
- Hence his persistent use of the dramatic monologue.
- The larger part of the volume consists of dramatic monologues.
- To Browning belongs the credit of having created a new poetic form,—the dramatic monologue.


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Lauren Conrad's summer must-haves
Lauren Conrad has shared her seasonal must-have fashion pieces.
The 29-year-old has made the successful transition from reality star to fashion designer over the years and is a style icon for many.
Sharing her guide to clothes for the warmer weather, Lauren reveals what pieces take her fancy.
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Lauren also compiled a list of items which fans can nab themselves. One of these was a cute Reformation Hali Dress, boasting a floral design, low ruffled back and a short structured skirt.
She isn't all about prints though as she also mentions a plain white Rebecca Minkoff Celestine Top.
"Don't be afraid to show off your shoulders this summer… This breezy little number from Rebecca Minkoff is sure to cool you down on a scorching day. Plus I can't get enough of the sweet laser-cut details," she added of the piece.
Another garment which shows off shoulders is a pale pink off-the-shoulder dress from ASOS, which Lauren describes as perfect for "twirling" in at a summer party.
Accessories make the cut too, with bags and shoes to match the outfits. A pair of Cornetti Recommone Gladiator Sandals match the ASOS dress, while a BAGGU Drawstring Bucket Bag would also go well with the piece.
"Bucket bags have been on my radar for the past couple of seasons. The prettiest shade of pink on one of my favourite bags is an award-winning combination," she gushed of the item.
© Cover Media


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LOVE IS THE DRUG: An Urban Love Story by TWEET
REAL ROXANNE IS BACK!!!!-----The glamour and politics of Hollywood swallow up a beautiful young film critic as she tries to resist the only man she's ever wanted--and his wife isn't far behind. A powerful love story filled with sex, drama and passion. This novel was written by KOLA BOOF during her soap opera days at "Days of Our Lives" and "Young and the Restless." She chose that it bepublished under the pen name "Tweet." LOVE IS THE DRUG is a FULL LENGTH NOVEL--374 pages. WARNING: Romantic love story contains EXPLICIT SEX, Mature Themes and Violence.
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The football world's longest off-season is almost at an end. Yes, the FFA Cup has provided something of a diversion, while Western Sydney Wanderers' epic adventures in the Asian Champions League have grabbed the attention of the whole country.
But football fans will breathe a healthy sigh of frustrated relief when the new A-League season kicks off again on Friday night with a clash between the two biggest clubs in the league – Melbourne Victory and the Wanderers – at Etihad Stadium.
It was more than five months ago that Brisbane Roar saw off the Wanderers in extra time in the grand final to take its third A-League title, courtesy of Henrique's decisive strike.
Since then we have had the Champions League final, a World Cup, and the opening games in qualifying for the next European Championships: the current English Premier League season (the last one of which finished after the A-League play-offs) is now more than six weeks old following its mid-August kick-off.
Whatever the future might hold – expansion, more matches, a longer season – there is nothing more pressing than the present, and the A-League's 10th season promises to be more intriguing than any other.
Will Brisbane, despite losing its talismanic striker Besart Berisha, be able to go back-to-back once again?
Will the Wanderers, their confidence and status boosted by their run to the final of the Asian Champions League, use that experience to go one better this season and take their first A-League title after being beaten in the past two championship deciders?
They are just two of the fascinating questions to be answered during the next seven months as the season develops its own rhythm and character, delivering a narrative crammed with as-yet-undreamt-of plot twists and turns.
All eyes are on the newly named Melbourne City following the takeover of Melbourne Heart by Manchester City, and its subsequent rebranding. John van 't Schip is quietly confident that the "other" Sky Blues can do well, but he is taking nothing for granted, warning this week that other teams and clubs were still some way in front of the transforming City.
Still, the addition of Premier League quality – with the likes of Damien Duff and Robert Koren added to the squad, alongside well-credentialled local newcomers such as Aaron Mooy and Erik Paartalu – should make City a highly competitive force from the outset.
The signing of Spanish superstar David Villa, who arrives next week for a 10-game guest stint, is merely icing on the cake.
Melbourne Victory has not stood still in the off-season either. Kevin Muscat's side lost narrowly to Brisbane in the semi-finals, and while it has lost important players, such as the on-loan striker James Troisi and the Ivory Coast full-back Adama Traore, the Navy Blues have recruited well and look to have a well-balanced squad with significant strength in depth.
Carl Valeri will bring maturity and quality to the defensive midfield role after more than a decade playing in Italy, most of it in Serie B. Valeri has also played at the World Cup in South Africa, and will have plenty of incentives to hit the ground running to try to force his way into contention for the Socceroos' Asian Cup squad.
Graham Arnold is the new man in charge at Sydney FC. Star Alessandro Del Piero has left after two seasons with the Sky Blues, and while they might not have signed anyone of Del Piero's glamour, Sydney has recruited broadly to give it strength in depth and provide Arnold with alternatives.
Alex Brosque should be a good addition to Sydney's front line, which has also added Bernie Ibini, once of the Central Coast Mariners, and former Perth Glory and Gold Coast United hitman Shane Smeltz.
Still, Arnold's stock in trade is defensive organisation: the former Socceroo striker and sometime national team manager always focuses on making his teams hard to beat, and Sydney should be championship challengers under its new boss.
In Perth, the faithful are still waiting for success. One grand final appearance aside (when former Rangers duo Ian Ferguson and Stuart Munro were at the helm), most of the past nine years have been a succession of false dawns for the men from the west.
There has been a significant personnel change in WA, and Youssouf Hersi, from the Wanderers, and Ruben Zadkovich, formerly of the Jets, should provide attacking flair and midfield energy as Kenny Lowe looks to change a culture of underachievement.
After a season in charge, Spaniard Josep Gombau knows a lot more about the A-League, and his players know a lot more about him and the structure and style in which he wants them to play. The Reds made the finals last year but exited swiftly, and the former Barcelona man will be anxious to go further this time.
Adelaide is always competitive and it has retained a South American core, augmented by the arrival of ex-Victory midfielder Jimmy Jeggo and former Newcastle wide man Craig Goodwin.
The Jets have gone through plenty of changes and have a new coach, Phil Stubbins, who finally gets the chance to prove his worth under his own steam. The Englishman was Aurelio Vidmar's assistant at Adelaide United and also coached in Thailand.
Central Coast always looks hard-pressed to match its previous competitiveness as each new season begins, but it inevitably does. Phil Moss✓ will be hoping that he can maintain its great record in the season ahead.