text:
Circulating microparticles: square the circle
Cytoplasmic and organelle biology
The present review summarizes current knowledge about microparticles (MPs) and provides a systematic overview of last 20 years of research on circulating MPs, with particular focus on their clinical relevance.
MPs are a heterogeneous population of cell-derived vesicles, with sizes ranging between 50 and 1000 nm. MPs are capable of transferring peptides, proteins, lipid components, microRNA, mRNA, and DNA from one cell to another without direct cell-to-cell contact. Growing evidence suggests that MPs present in peripheral blood and body fluids contribute to the development and progression of cancer, and are of pathophysiological relevance for autoimmune, inflammatory, infectious, cardiovascular, hematological, and other diseases. MPs have large diagnostic potential as biomarkers; however, due to current technological limitations in purification of MPs and an absence of standardized methods of MP detection, challenges remain in validating the potential of MPs as a non-invasive and early diagnostic platform.
Improvements in the effective deciphering of MP molecular signatures will be critical not only for diagnostics, but also for the evaluation of treatment regimens and predicting disease outcomes.
The present review summarizes information concerning microparticles (MPs), covering the clinical aspects of circulating MPs, recent advances and technological developments in this field.
Several recent reviews have concentrated on specific aspects of cellular vesicles biology, focusing primarily on exosomes (subset of cellular vesicles with size < 100 nm) and the mechanisms involved in cellular vesicles release and signaling
Results and discussion
MPs: attempts to define
MPs are described as a heterogeneous population of membrane-delimitated vesicles 50–1000 nm in size released from the cells in which they form and retaining certain antigens of their cells of origin
The size of MPs (50 to 1000 nm), their lipid composition, and their irregular shape and density are major parameters that separate them from exosomes (usually of diameter < 100 nm and lower density – 1.13-1.19 g/mL) and apoptotic bodies (much larger vesicles released at the final steps of apoptosis and normally 1000–3000 nm in size)
Additional file 1
Range of MP sizes in different publications.
Click here for file
Methods of MPs characterization
Isolation of MPs typically involves a combination of centrifugation and size-based filtration followed by characterization using flow cytometry, electron microscopy, Western blotting or proteomics. Isolation of MPs from the peripheral blood of patients or healthy controls starts with drawing blood into the tubes with different anticoagulants: sodium citrate, acid-citrate-dextrose, EDTA salt, or heparin. Sodium citrate is the most widely used anticoagulant
Research focused on elucidating MP composition and functional activity is hampered by the complexity of the biological fluids where MPs are present and the small size of MPs
The protein content of MPs is usually ascertained by Western blotting and proteomic approaches
Current flow cytometry methods utilize both fluorescence probes and light scattering. Quantification of MPs by flow cytometry shows good correlation with the relative light scattering intensities determined by dynamic light scattering
Figure 1
Distribution of Dragon Green-conjugated beads in sizes of 190 nm, 510 nm, and 730 nm (images acquired with a SORP Aria 2 cytometer, Flow and Imaging Cytometry Resource, PCMM, Boston Children's Hospital)
Distribution of Dragon Green-conjugated beads in sizes of 190 nm, 510 nm, and 730 nm (images acquired with a SORP Aria 2 cytometer, Flow and Imaging Cytometry Resource, PCMM, Boston Children's Hospital). This figure was included in an advanced abstract as part of the Proceedings of the International Workshop on Applied Cytometry (2012).
Figure 2
MP images (erythrocyte-derived MPs) acquired with an Imagestream 100 (40x objective) (Amnis Inc, Seattle, USA)
MP images (erythrocyte-derived MPs) acquired with an Imagestream 100 (40x objective) (Amnis Inc, Seattle, USA). A dotblot showing a mixture of erythrocytes and erythrocyte-derived microparticles (X-axis-brightfield area; Y-axis-brightfield aspect ratio of intensity). a.) Multiple erythrocytes (region R3 on dotblot); b.) Single erythrocytes (region R2 on dotblot); c.) Microparticles (brightfield) (region R1 on dotblot); d.) Microparticles stained with calcein AM (images from calcein AM-channel are particles taken from R1-region).
Additional file 2
References for Table 1 (Summary of some methods applied for MPs research).
Click here for file
Origin of MPs
MPs have been identified in human plasma, peripheral blood, cord blood, urine, saliva and cerebrospinal fluid
To characterize the cellular origin of MPs in peripheral blood, the most common approach is to stain MPs with fluorescently-labeled AB directed against antigens of parental cells (for example CD41, CD61 and platelet-activation marker CD62 for platelets; glycophorin for erythrocytes; CD45 for lymphocytes; CD14 for monocytes; and so on) and to perform subsequent analysis by flow cytometry. However, a large variety of CD markers have been used by different groups to characterize background and activation of MPs derived from endothelial cells (CD31, CD34, CD62E, CD51, CD105, CD144, CD146) versus platelets (CD41, CD41a, CD42a, CD42b, CD61, CD62P) may have led to inconsistency in the functional characterization of MPs populations (reviewed in
Shedding (ectocytosis) and MP content
Though MP shedding is enhanced upon cell activation, constitutive ectocytosis is a permanent ongoing process
Plasma membrane remodelling is a critical event during apoptosis and cell activation, and enzymes that regulate this process also regulate MP production
Figure 3
Basic scheme of MP formation via reverse budding
Basic scheme of MP formation via reverse budding. A. Activated cells release MPs in response to Ca++ agonists. Increased concentration of Ca++ alter the asymmetric PS distribution of the plasma membrane, activate kinases, inhibit phosphatases and activate calpain, which leads to reorganization of cytoskeleton and increased MPs production. B. MP formation during the early stages of apoptosis is associated with GTP-bound Rho proteins, which activate the ROCK-I kinase. This kinase is involved in cortical myosin-II contraction, detachment of the plasma membrane from the cytoskeleton, and release of MPs that have hijacked cytoplasmic components, nucleic acids, and membrane antigens.
Several other enzymes possibly involved in MPs formation and activity include aminophospholipid translocase, and other members of the floppase family, as well as protein disulfide isomerase and acid sphingomyelinase
Enhanced release of MPs is associated with diverse stimuli including hormones, fatty acids, reactive oxygen species (e.g. hydrogen peroxide)
MPs also carry all types of nucleic acid molecules, including mRNA and DNA fragments
Functional activities of MPs: interaction with homologous or heterologous cells
As outlined above, MP production is a tightly regulated and selective process, suggesting that MPs may be important mediators of cell-to-cell communication. MPs can be internalized in a dose-dependent manner by macrophages, endothelial cells and other cell types (an example of MP internalization by hCMEC/D3 cells is shown in Figure
Figure 4
An epifluorescence microscopy image shows that hCMEC/D3 cells have internalized small MPs (arrowheads), which had been purified from human glioma cells treated with TRAIL (100 units/ml) and stained with PKH-67 (Sigma, USA) before addition to the hCMEC/D3 cells
An epifluorescence microscopy image shows that hCMEC/D3 cells have internalized small MPs (arrowheads), which had been purified from human glioma cells treated with TRAIL (100 units/ml) and stained with PKH-67 (Sigma, USA) before addition to the hCMEC/D3 cells. MPs that are attached to the cell surface are out of focus (representative photo from Z-stack collection). Objective Plan Apo x60/1.4. Bar 5 μm.
The MPs express adhesion molecules on their surface, which may influence the probability of their capture by target cells and mediate MPs effects on cell behavior
Lung-derived MPs have been shown to transfer mRNA to marrow cells
A growing body of evidence supports an important role for MPs in the induction of apoptosis. MPs released at the early stages of apoptosis do not contain organelles and their size is smaller than 1 μm; however, they sediment at a lower acceleration than exosomes
Circulating MPs
The level of circulating MPs depends on the balance between their rates of formation and clearance. Clearance of MPs occurs through several main mechanisms. The major one is degradation due to the action of phospholipases and proteases
While low MP concentrations can be detected in the blood and body fluids of healthy subjects
Additional file 3
MP-based risk stratification of some pathological states.
Click here for file
Additional file 4
Supplemental Table. Changes in MP levels in peripheral blood of patients in response to treatments.
Click here for file
Additional file 5
References for Table 2 (MP levels in the plasma of healthy controls).
Click here for file
Additional file 6
References for Table 3 (MP levels in the plasma and body fluids of patients with cancer).
Click here for file
Additional file 7
References for Table 4 (MP levels in the plasma and body fluids of patients with different disorders).
Click here for file
The association of elevated levels of certain MP subtypes with specific disease states may also have therapeutic implications. An interesting possibility is the use of
These findings have ignited interest to MPs as possible biomarkers for diagnostics and evaluation of efficiency of a therapeutic strategy.
MPs in cancer
Cancer cell-derived MPs have been studied intensively in recent years, and their potential as diagnostic and prognostic tools has been described
MPs shed by tumor cells serve as a profound additional pathway for drug release
The contribution of platelet-derived MPs to hematogeneous cancer metastasis is tied to their procoagulant activity
MPs and vascular diseases
Platelet-derived MPs have been extensively investigated for their ability to induce coagulation and participate in thrombosis because they display PS and other negatively charged phospholipids that provide binding sites for activated coagulation factors
Since endothelial MPs from patients with metabolic disorders induce endothelial dysfunction in animal models
MPs and infectious diseases
Bacterial virulence factors such as the M1 protein from
ErMP levels are significantly increased in the blood of malaria patients with coma or severe malaria
As methods for isolating and characterizating MPs advance, it is anticipated better understanding of the mechanisms of MP formation and functional activity will be achieved in near future (a current overview of MP activity is summarized in Figure
Figure 5
Potential mechanisms of MP action
Potential mechanisms of MP action.
AB: Antibody; ABCA1: ATP binding cassete transporter A1; ABCB1: ATP binding cassete transporter B1; ADAM10: A disintegrin and metalloproteinase domain-containing protein 10; ARF6: ADP-ribosylation factor 6; Allo-HSCT: Allogeneic hematopoietic stem cell transplantation; ARFCES: Carcinoembryonic antigen; ASCT: Allogeneic stem cell transplantation; BALF: Bronchoalveolar lavage fluid; calcein AM: Acetometoxy derivate of calcein; CCR5: C-C chemokine receptor type 5; CXCL12: Chemokine (C-X-C motif) ligand 12; CXCR-4: C-X-C chemokine receptor type 4; DC: Dendritic cell; EDTA: Ethylenediaminetetraacetic acid; EM: Electronic microscopy; EGFR: Epidermal growth factor receptor; EMPs: Endothelial microparticles; EpCAM: Epithelial cell adhesion molecule; Er-MPs: Erythrocyte-derived microparticles; ERK: Extracellular signal-regulated kinase; Fas: CD95; FasL: Fas ligand; FMD: Flow-mediated vasodilatation; GVHD: Graft-versus-host disease; HER2: Human epidermal growth receptor 2; HIV: Human immunodeficiency virus; HSP: Heat shock protein; HUVEC: Human umbilical vein endothelial cell; ICAM-1: Intercellular adhesion molecule 1; LLC: Lewis lung carcinoma; LPS: Lipopolysaccharide; MDR: Multiple drug resistance; mRNA: Messenger RNA; miRNA: microRNA; MMPs: Metalloproteinase; MPs: Microparticles; NTA: Nanoparticle tracking assay; PAF: Platelet-activating factor; PBMCs: Peripheral blood mononuclear cells; PCOS: Polycystic ovary syndrome; P-gp: P-glycoprotein; PMPs: Platelet-derived microparticles; PMNs: Polympophnonuclear neutrophils; PS: Phosphatidylserine; RNA: Ribonucleic acid; sPLA2: Secretory phospholipase A2; PTEN: Phosphatase and tensin homolog; ROCK-1: Kinase; Rho-1: Associated kinase; SIRS: Systemic inflammatory response syndrome; SLE: Systemic lupus erythematosus; STAT: Signal transducer and activator of transcription; TF: Tissue factor; TLR: Toll-like receptor; TNF-α: Tumor-necrotic factor alpha; TRAIL: TNF-related apoptosis-induced ligand; TRM: Transplantation-related mortality; TSG101: Tumor specific antigen 101; VEGF: Vascular endothelial growth factor.
Competing interests
LD is employed by Becton Dickinson Biosciences Inc. Other authors do not have any competing interests.
Authors' contributions
NSB and IAV wrote the first draft. EFK, MB, JNHS, EDP and LD critically reviewed a manuscript and contributed towards figures. All authors read and approved the final manuscript.
We are thankful to Luke Jasenosky and Aleksandra Gorelova (Harvard University) for editorial help with the manuscript. NSB was supported by a Harvard Pilot Grant and the Immune Disease Institute, and IAV was supported by Russian Foundation for Basic Research grants 11-01517a and 11-01749a.
Exosomes as intercellular signaling organelles involved in health and disease: basic science and clinical applications
Exosomes: extracellular organelles important in intracellular communication
Exosome secretion: molecular mechanisms and roles in immune responses
Exosomes: immune properties and potential clinical implementations
Exosomes and other microvesicles in infection biology: organelles with unanticipated phenotypes
Role of exosomes/microvesicles in the nervous system and use in emerging therapies
Microparticles: protagonists of a novel communication network for intracellular information exchange
Classification, functions, and clinical relevance of extracellular vesicles
Vesicles as carriers of virulence factors in parasitic protozoan diseases
Formation and fate of platelet microparticles
Microparticles as regulators of inflammation: novel players of cellular crosstalk in the rheumatic diseases
Characterizing blood microparticles: technical aspects and challenges
The role of microparticles in the pathogenesis of rheumatic diseases
Microparticles: a critical component in the nexus between inflammation, immunity and thrombosis
Microparticles, malignancy and thrombosis
Exosome-based immunotherapy
Pathophysiologic implications of membrane phospholipid asymmetry in blood cells
Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules
Proteomic analysis of denritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles
Flow cytometric analysis of circulating microparticles in plasma
Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles
Exosomes: composition, biogenesis and function
Measuring circulating cell-derived microparticles
Flow cytometric measurement of microparticles: pitfalls and protocol modifications
Centrifugation is a crucial step impacting microparticle measurement
Detection of circulating microparticles by flow cytometry: influence of centrifugation, filtration of buffer, and freezing
Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters
Haemolysis during sample preparation alters microRNA content of plasma
Microparticle sizing by dynamic light scattering in fresh-frozen plasma
Flow cytometric detection of endothelial microparticles (EMP): effects of centrifugation and storage alter with the phenotype studied
Optical and non-optical methods for detection and characterization of microparticles and exosomes
Ectosomes released by human neutrophils are specialized functional units
Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients
Comparison of three methods for isolation of urinary microvesicles to identify biomarkers of nephrotic syndrome
The induction of matrix metalloproteinase and cytokine expression in synovial fibroblasts stimulated with immune cell microparticles
Tumor-derived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner
Liver cell-derived microparticles activate hedgehog signaling and alter gene expression in hepatic endothelial cells
Isolation and characterization of microparticles in sputum from cystic fibrosis patients
T-cadherin is present on endothelial microparticles and is elevated in plasma in early atherosclerosis
Isolated microparticles from peripheral blood and body fluids as observed by scanning electron microscope
Endothelial microparticle formation by angiotensin II is via Ang II Receptor type I/NADPH oxidase/Rho kinase pathways targeted to lipid rafts
Microparticles: a new tool for plasma membrane sub-cellular proteomic
Plasma-derived microparticles for biomarker discovery
Identification and proteomic profiling of exosomes in human cerebrospinal fluid
Single vs swarm detection of microparticles and exosomes by flow cytometry
Microparticle size and its relation to composition, functional activity, and clinical significance
Pre-analytical and methodological challenges in red blood cell microparticle proteomics
Novel test for microparticles in platelet-rich plasma and platelet concentrates using dynamic light scattering
Microparticle-associated tissue factor activity: a link between cancer and thrombosis?
Increased microparticle tissue factor activity in cancer patients with venous thromboembolism
Impedance-based flow cytometry for the measurement of microparticles
Measurement of platelet microparticles
Differential stimulation of monocytic cells results in distinct populations of microparticles
Monocyte/macrophage-derived microparticles up-regulate inflammatory mediator synthesis by human airway epithelial cells
Bio-maleimide as a generic stain for detection and quantitation of microparticles
Proinvasive properties of ovarian cancer ascites-derived membrane vesicles
Circulating microparticles: pathophysiology and clinical implications
Isolation and identification of potential urinary microparticle biomarkers of bladder cancer
Proteomics analysis of a podocyte vesicle-enriched fraction from normal human and pathological urines
Cell-derived vesicles exposing coagulant tissue factor in saliva
Microparticles (MPs), tissue factor (TF) and tissue factor inhibitor (TFPI) in cord blood plasma. A preliminary study and literature survey of procoagulant properties of MPs
Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndrome
High levels of circulating leukocyte microparticles are associated with better outcome in acute respiratory distress syndrome
Apoptosis in the atherosclerotic plaque: quantitative and qualitative aspects
Shed membrane microparticles with procoagulant potential in human atherosclerotic plaques: a role of apoptosis in plaque thrombogenecity
Microparticles and type 2 diabetes
CR1, CD35 in synovial fluid from patients with inflammatory joint diseases
Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes
Activated complement compounds and complement activator molecules on the surface of cell-derived microparticles in patients with rheumatoid arthritis and healthy individuals
Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
Increased vitreous shedding of microparticles in proliferative diabetic retinopathy stimulates endothelial proliferation
Differential contributions of monocyte- and platelet-derived microparticles towards thrombin generation and fibrin formation and stability
Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation
Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles
Platelet-derived microparticles-an updated perspective
Cellular origin of platelet-derived microparticles
Leukocyte-derived microparticles in vascular homeostasis
Ectosomes as modulators of inflammation and immunity
Complement proteins C5b-9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity
Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles
Selective localization of matrix metalloproteinase 9, beta1 integrins, and human lymphocyte antigen class I molecules on membrane vesicles shed by 8701-BC breast carcinoma cells
The phospholipid composition and cholesterol content of platelet-derived microparticles: a comparison with platelet membrane fractions
Comparative proteomic analysis of PAI-1 and TNF-alpha-derived endothelial microparticles
Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis
Comparative analysis of platelet-derived microparticles reveals differences in their amount and proteome depending on the platelet stimulus
Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets
Expression, activation, and function of integrin alphaM/beta2 (Mac-1) on neutrophil-derived microparticles
Tissue factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation
Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells
Regulation of transbilayer plasma membrane phospholipid asymmetry
Transmembrane phospholipid distribution in blood cells: control mechanisms and pathophysiological significance
Platelet microparticles are heterogeneous and highly dependent on the activation mechanism: studies using a new digital flow cytometer
Phosphatidylserine surface expression and integrin alpha IIb beta 3 activity on thrombin/convulxin stimulated platelets/particles of different sizes
Analysis of tissue factor positive microparticles
Platelet adhesion enhances the glycoprotein VI-dependent procoagulant response: involvement of p38 MAP kinase and calpain
Sickle blood contains tissue factor positive microparticles derived from endothelial cells and monocytes
Circulating microparticles are associated with vascular dysfunction in patients with end-stage renal failure
Evidence that agonist-activated activation of calpain causes the shedding of procoagulant-containing microvesicles from the membrane of aggregating platelets
ADP-ribosylation factor 6 regulates tumorigenic and invasive properties
Significance of capacitative Ca2+ entry in the regulation of phosphatydylcholine expression at the surface of stimulated cells
Cellular mechanisms underlying the formation of circulating microparticles
Exposure of platelet membrane phosphatidylserine regulates blood coagulation
Microparticles: targets and tools in cardiovascular disease
The majority of circulating platelet-derived microparticles fail to bind annexin V, lack phospholipid-procoagulant activity and demonstrate greater expression of glycoprotein Ib
Circulating mesenchymal stem cells microparticles in patients with cerebrovascular disease
Circulating microparticles in systemic lupus erythematosus
Identification of a factor that links apoptotic cells to phagocytes
Identification of Tim4 as a phosphatidylserine receptor
Cellular microparticles: What are they bad or good for?
Microparticles in cardiovascular diseases
Extracellular protein disulfide isomerase regulates coagulation on endothelial cells through modulation of phosphatidylserine exposure
P2X7 receptor signaling contributes to tissue factor-dependent thrombosis in mice
Acid sphingomyelinase activity triggers microparticle release from glial cells
Loss of plasma membrane phospholipid asymmetry requires raft integrity. Role of transient receptor potential channels and ERK pathway
Receptors, rafts, and microvesicles in thrombosis and inflammation
Identification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth factor 8-associated microvesicles
Membrane vesicles as conveyors of immune responses
Effects of progesterone and estradiol sex hormones on the release of microparticles by RAW 264.7 macrophages stimulated by Poly (I:C)
Postprandial changes in phospholipid composition of circulating microparticles are not associated with coagulation activation
High-shear stress-induced activation of platelets and microparticles enhances expression of cell adhesion molecules in THP-1 and endothelial cells
Circulating microparticles and endogenous estrogen in newly menopausal women
Measurement of platelet-derived microparticle levels using an enzyme-linked immunosorbent assay in polymyositis and dermatomyosistis patients
Insulin inhibits tissue factor expression in monocytes
Horizontal transfer of oncogenes by uptake of apoptotic bodies
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
Platelets and platelet-like particles mediate intercellular RNA transfer
Detection of microRNA expression in human peripheral blood microvesicles
Potential role of miRNAs in developmental haemostasis
Microparticles: major transport vehicles for distinct miRNAs in circulation
Microvesicles released from rat adipocytes and harboring glycophosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis
Microvesicles derived from endothelial progenitor cells protect the kidney from ischemia-reperfusion injury by microRNA-dependent reprogramming of resident renal cells
Atheroprotective communication between endothelial cells and smooth muscle cells through miRNA
Endotoxins stimulate neuthrophil adhesion followed by synthesis and release of platelet-activating factor in microparticles
Neuthrophil priming, caused by cell membranes and microvesicles in packed red blood cell units, is abrogated by leukocyte depletion at collection
Increased production of leukocyte microparticles with enhanced expression of adhesion molecules from activated polymorphnonuclear leukocytes in severely injured patients
Microparticles from ovarian carcinomas are shed into ascites and promote cell migration
Microparticles from human atherosclerotic plaques promote endothelial ICAM-1-dependent monocyte adhesion and transendothelial migration
Erythrocyte-derived ectosomes have immunosuppressive properties
Microparticles released by human neutrophils adhere to erythrocytes in the presence of complement
Transfer of the chemokine receptor CCR5 between cells by membrane-derived microparticles: a mechanism for cellular immunodeficiency virus 1 infection
Blood-borne tissue factor: another view of thrombosis
Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape
Microvesicles as mediators of intercellular communication in cancer-the emerging science of cellular "debris"
Platelet and megakariocyte-derived microparticles transfer CXCR4 receptor to CXCR-null cells and make them susceptible to infection by X4-HIV
Delivery of microRNA-126 by apoptotic bodies induces CXCl12-dependent vascular protection
Induction of apoptosis in circulating angiogenic cells by microparticles
Microvesicle entry into marrow cells mediates tissue-specific changes in mRNA by direct delivery of mRNA and induction of transcription
The dynamic stem cell microenvironment is orchestrated by microvesicle-mediated transfer of genetic information
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery
Human liver stem cell-derived microvesicles accelerate hepatic regeneration in hepatectomized rats
Transfer of microRNAs by embryonic stem cell microvesicles
Microvesicles derived from adult human bone marrow and tissue specific mesenchymal stem cells shuttle selected pattern of miRNAs
Monocyte derived microvesicles deliver a cell death message via encapsulated caspase-1
Cell-derived microparticles contain caspase 3
Inhibition of microparticle release triggers endothelial cell apoptosis and detachment
Platelet microparticles contain active caspase 3
Biologically active Fas antigen and its cognate ligand are expressed on plasma membrane–derived extracellular vesicles
Induction of lymphocyte apoptosis by tumor cell secretion of FASL-bearing microvesicles
Tumour-derived exosomes and their role in cancer-associated T-cell signalling defects
Fas ligand-positive membranous vesicles isolated from sera of patients with oral cancer induce apoptosis of activated T lymphocytes
Secretory phospholipase A2 generates the novel lipid mediator lysophosphatidic acid in membrane microvesicles shed from activated cells
Liver Kupfer cells rapidly remove red blood-derived vesicles from the circulation by scavenger receptors
Activated platelet-derived microparticles in thalassemia
Uptake of inhalable microparticles affects defence responses of macrophages infected with
Studies of factors regulating the aging of human erythrocytes. III. Metabolism and fate of erythrocytic vesicles
Clearance of platelet microparticles
IgM promotes the clearance of small particles and apoptotic microparticles by macrophages
Endothelial cell-derived microparticles loaded with iron oxide nanoparticles: feasibility of MR imaging monitoring in mice
Exosomal-like vesicles are present in human blood plasma
Gender-specific and menstrual cycle dependent differences in circulating microparticles
A filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma
Increased CD62e + endothelial microparticles levels predict poor outcome in pulmonary hypertension patients
Prognostic value of endothelial microparticles in patients with heart failure
Circulating CD31+/Annexin V + microparticles correlate with cardiovascular outcomes
Increased levels of circulating microparticles in primary Sjogren's syndrome, systemic lupus erythematosus and rheumatoid arthritis and relation with disease activity
Interferon-beta1a reduces plasma CD31+ endothelial microparticles (CD31 + EMP) in multiple sclerosis
The effects of high dose interferon-beta1 on plasma microparticles: correlation with MRI parameters
Enhancement of vascularization and granulation tissue formation by growth factors in human platelet-rich plasma-containing fragmin/protamine microparticles
Microparticles in angiogenesis: therapeutic potential
Protective effects of vitamin C on endothelium damage and platelet activation during myocardial infarction in patients with sustained generation of circulating microparticles
Effects of miglitol in platelet-derived microparticle, adiponectin, and selectin level in patients with type 2 diabetes mellitus
New immunophenotype of circulating endothelial progenitor cells and endothelial microparticles in patients with erectile dysfunction and metabolic syndrome: effects of taladafil administration
Circulating red cell-derived microparticles in human malaria
Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers
Membrane vesicles in ovarian cancer fluids: a new potential marker
Circulating tumour-derived microvesicles in plasma of gastric cancer patients
All circulating EpCAM+CK+CD45- objects predict overall survival in castration-resistant prostate cancer
Elevated levels of circulating platelet microparticles, VEGF, IL-6 and RANTES in patients with gastric cancer: possible role of a metastasis predictor
Crosstalk between cancer and haemostasis: implications for cancer biology and cancer-associated thrombosis with focus on tissue factor
Microparticles and exosomes in gynecologic neoplasia
Microvesicles: mediators of extracellular communication during cancer progression
Cancer and thrombosis: from molecular mechanisms to clinical presentations
Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells
Microvesicles released from human renal cancer stem cells stimulate angiogenesis and formation of lung premetastatic niche
Expulsion of small molecules in vesicles shed by cancer cells: association with gene expression and chemosensitivity profiles
Microparticle-associated nucleic acids mediate trait dominance in cancer
Different modalities of intercellular membrane exchanges mediate cell-to-cell P-glycoprotein transfers in MCF-7 breast cancer cells
Pathophysiologic significance of procoagulant microvesicles in cancer disease and progression
Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer
Platelet microparticles: a potential predictive factor of survival in hormone-refractory prostate cancer patients treated with docetaxel-based chemotherapy
Microparticles in vascular diseases
The role of microparticles in inflammation and thrombosis
Shedding of the matrix metalloproteinases MMP-2, MMP-9, and MT1-MMP as membrane vesicle-associated components by endothelial cells
Platelets stimulate proliferation of bone cells: involvement of platelet-derived growth factor, microparticles and membranes
Platelet-derived microparticles induce angiogenesis and stimulate post-ischemic revascularization
Microparticles of human atherosclerotic plaques enhance the shedding of tumor necrosis factor-alpha converting enzyme/ADAM 17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1
Elevated levels of circulating procoagulant microparticles in patients with paroxysmal nocturnal hemoglobinuria and aplastic anemia
Thrombosis in patients with paroxysmal noctural hemoglobinuria is associated with markedly elevated plasma levels of leukocyte-derived tissue factor
Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria
Endothelial dysfunction caused by circulating microparticles from patients with metabolic syndrome
Monocyte vesiculation is a possible mechanism for dissemination of membrane-associated procoagulant activities and adhesion molecules after stimulation by lipoplysaccharide
Stimulation of blood mononuclear cells with bacterial virulence factors leads to the release of pro-coagulant and pro-inflammatory microparticles
The clinical and functional relevance of microparticles induced by activated protein C treatment in sepsis
Enhanced production of endothelial microparticles with increased binding to leukocytes in patients with severe systemic inflammatory response syndrome
Complement activation on platelet-leukocyte complexes and microparticles in enterohemorrahagic Escherichia coli-induced hemolytic uremic syndrome
Circulating microparticles from septic shock patients exert differential tissue expression of enzymes related to inflammation and oxidative stress
Detrimental hemodynamic and inflammatory effects of microparticles originating from septic rats
Circulating endothelial microparticles in malawian children with severe falciparum malaria complicated with coma
Microvesiculation and cell interactions at the brain-endothelial interface in cerebral malaria pathogenesis
Maurer's clefts of
Platelet microparticles: a new player in malaria parasite cytoadherence to human brain endothelium
Genesis of and trafficking to the Maurer's clefts of
An exosome-based secretion pathway is responsible for protein export from
Interactions of
Periodontal infection and atherosclerosis: mere associations?
Identification of leishmania fructose-1,6-biphosphate aldolase as a novel activator of host macrophage Src homology 2 domain containing protein tyrosine phosphatase SHP-1
Localization and induction of the A2 virulence factor in
Extracellular vesicles from


text:
THE head of a city manufacturing think-tank has been sacked after being arrested over allegations of making pornography.
James Bentley, a former partner with KPMG, had his contract with the Manufacturing Foundation terminated following his arrest by specialist police officers on Friday.
He was questioned at Solihull police station about allegations of making and possessing indecent images, by the West Midlands High Tech Crime Unit before being released on bail.
The foundation board met on Monday and terminated his contract. Mr Bentley was director of the Foundation, a not-for-profit organisation giving policy advice to regional and central government.
Alan Curtis, from the Warwick Manufacturing Group, at the University of Warwick, and chairman of the foundation board, refused to comment.
There was no comment from AWM and the foundation's website was "offline for routine maintenance".
Mr Bentley's work has included master classes to 14 and 15-year-olds as part of their GCSE business studies.
A West Midlands Police spokeswoman said: "A 54-year-old man was arrested in Solihull on Friday morning on suspicion of making and possessing indecent images.
"He was released on police bail pending further inquiries."
Mr Bentley was unavailable for comment.

subset: commoncrawl


text: Bridge Night scene with spot light -Royalty Free Image License: Standard LicenseStandard Royalty Free License Night scene of Liede bridge with brilliant spot light, Photo taken in Guangzhou city, Guangdong province of China 243 views Ray Woo An experience stock photographer based in China. The main topics of my works... [more] info Author Page Portfolio: 3124 images. Updates / RSS Network Image updated—August 7, 2013 Keywords architecture, beauty, black, bridge, building, business, China, Citizen, city, color, colorful, colors, colourful, evening, Guangdong, Guangzhou, light, lighting, modern, night, night scene, place, river, scene, sky, spot, track, urban, water Image Categories Architecture | Building Image #1865 Size Type File Info Price ( US Dollars $ )50% off Bloggeejpg4.63" × 6.94" @ 72 dpi333 × 500px2.001.00 Smalljpg9.26" × 13.89" @ 72 dpi667 × 1000px4.002.00 Mediumjpg5.55" × 8.33" @ 300 dpi1666 × 2500px8.004.00 Largejpg8.94" × 13.41" @ 300 dpi2681 × 4022px12.006.00 End User License Agreement View Cart (0.00) Symbiostock Community Symbiostock CommunityNetwork with illustrators, photographers, and designers. Symbiostock on TwitterSymbiostock news. Symbiostock on FacebookCommunity maintained Facebook hub. Symbiostock.infoSearch images on the Symbiostock network. Symbiostock SearchSearch Symbiostock images and network. Symbioguides.comSymbiostock knowledge base. Symbiostock.comSell your images and network with fellow microstock professionals. ClipArtof.comHigh Resolution Stock Illustrations & Clip Art ClipArtIllustration.comFirst Symbiostock Site, and home of the Orange Man Microstockgroup.comA meeting place for microstock professionals.


text:
The year 2013 is off to a promising start — but don't get used to it. Charlie Coglan said he's hoping for nine months in a row with modest job growth, a streak Maine hasn't seen since 2007. Ahead of his state forecast for the New England Economic Partnership conference …
Comments for: Colgan: Jobs recovery expected in 2016
Posted May 23, 2013, at 7:20 a.m.
Guidelines for posting on bangordailynews.com
The Bangor Daily News encourages comments about stories, but you must follow our terms of service.
- Keep it civil and stay on topic
- No vulgarity, racial slurs, name-calling or personal attacks.
- People who harass others or joke about tragedies will be blocked.
It should be noted this is not a comprehensive list.
- Don't insult one another or the subjects of BDN stories. You may be ticked off at what someone did or said, but you can explain that without resorting to name-calling or obscenity. Remember that young people are on this site too.
- That nasty line that makes your buddies go "haw, haw"? You're better than that.
- Comments should be your own work, not copied and pasted from elsewhere, though brief quoted passages to make your point are fine. And please don't use all capital letters. There's no need to shout.
- Stay focused on the issue in the article. Off-topic posts will be deleted.
- This isn't a forum to chat about your personal life.
- Some ideas — the fate of the earth, abortion, gun control, the nature of God — aren't going to be resolved in a reader forum, so no need to get frustrated if others don't see your point of view. State your position once and leave it at that.
- If you mouse over each comment you will see a "Flag" button. Please use it if you feel comments on the site are out of line. Quite often, we won't see a comment until it's reported.
- Questions about why a comment was — or wasn't — deleted? E-mail us at email@example.com and let us know your username and where you posted or saw the comment.


text:
By the nature of their jobs, firefighters must be dedicated individuals, willing to put their lives at risk for the safety of the community. Even among such committed men and women, there will always be those who stand out. Municipalities and governments all have unique sets of awards and honors periodically granted to recognize valor and effort that goes above the call of duty. While these recognitions certainly differ between departments, several honors are basically universal.
The Award of Merit
The Award of Merit--sometimes also called the "Merit Award" or "Medal of Merit"--is an award given to a firefighter for saving a life when there is no risk to their own life. It is also occasionally presented for specific acts of bravery, initiative or capability during the performance of one's duties. Interestingly, some municipalities will also award this to civilians who assist the fire department in some significant way during an emergency.
The Recognition Award
This relatively unique award is granted to a firefighter who, by his exemplary actions while in the line of duty, calls favorable attention from the community to their unit, their department and the profession of firefighting. There are a number of varieties of this award. For example, some communities may have it broken down to three or four separate awards (for instance, Officer of the Year or Chief of the Year), while others (such as the City of Lubbock, Texas) may name the award after prominent members of their department.
The Lifesaving Award
This particular award has several names differing from department to department, like the Medal of Honor or the Life Saving Medal. This award is specifically granted to those firefighters who save the life of another at great risk to personal injury or even death. An interesting variation of this medal is the EMS Lifesaving Award, which is granted to firefighting personnel who manage to resuscitate a breathless victim, who goes on to survive the ordeal.
The Unit Award
This award is given to members of a unit (comprised of two or more firefighters) who have conducted themselves as a whole in a manner worthy of recognition. The Unit Award is specifically granted for exemplary teamwork in overcoming a common obstacle while in the performance of duty. Variations include the Unit of the Year Award, the Unit Citation Award and the Meritorious Unit Award. Unlike the other awards listed, which are individual awards, the Unit Award is granted to each member of the unit.
Related Searches
- Photo Credit Hemera Technologies/AbleStock.com/Getty Images


text:
Woven leather belt
Woven leather belt
Thin woven leather belt with end tip. To be worn tied around the waist or fastened with a classic metal buckle.
Contact us for more information
Product name: BAZAR
Product code: WE5501047406005
Distributed by Max Mara S.r.l., registered office in Reggio Emilia (Italy), Via Giulia Maramotti 4, 42124 (IT)
See more bags, jewelry, scarves and foulards.
Shipping is always free. You will receive your order in approximately 4 to 8 business days. We will send you the Tracking Number to follow the delivery on the UPS website.
Returns can be requested within 14 days from when you receive your order. Return shipping is also free if you use our pre-printed label.


text:
EthicsDaily.com Videos Introduce Global BaptistsJuly 31, 2015
During the Baptist World Congress in Durban, South Africa, EthicsDaily.com created a series of videos featuring various global Baptist leaders. An article provides a quick overview of the videos. The piece, EthicsDaily.com Videos Introduce Global Baptists, includes a photo I took of Cliff Vaughn filming as Robert Parham interviewed Edward Dima of South Sudan.
I appeared in one video as Robert Parham and I discussed Baptist-Muslim relations during our time in Dubai. The whole set of about two dozen videos runs just over 35 minutes. They're all good, but below are a few of my favorites. It is good to hear from Baptists in other countries and learn from their experiences and ideas.
Robert Parham and Edward Dima: Baptists in South Sudan from EthicsDaily on Vimeo.
Robert Parham and Dimitrina Oprenova: Bulgarian Baptists from EthicsDaily on Vimeo.


text:
R. Olson (RO) Photographer
Mishi ParkModel Female Puyallup, Washington, US
My MM URL: http://www.modelmayhem.com/mishipark
Mayhem # 2981470
About MeMy name is Mishi Park, I was born and raised in Western Washington and have traveled to many exciting places around the world. I am restarting my modeling career after taking a short break with my two beautiful children. I have over 10 years of modeling experience and am very easy to work with. I have reasonable rates for implied shoots. Please feel free to contact me if you are intrested in shooting. You may contact me by e mail. My e mail address is firstname.lastname@example.com. Thank you for taking the time to view my work. I look forward to working with you soon. Please add me on facebook, the account is under Mishi Park.
Have you worked with Mishi Park? Add Credits for Mishi Park! >>
Credit NotesCharles Valentin with Image is Everything
R. Olson (RO) Photographer
Sam Rambo Photographer
Fostography Concepts Photographer
UpDog Photos Photographer
Jamie Velandra Photographer
NWEXPOSED Photography Photographer
Loading Content


text:
Meanwhile, QB Ryan Leaf was added to the injury list on Wednesday afternoon after suffering a sprained right wrist. The severity of his wrist sprain is not yet known, but Buccaneers should announce what they are going to list Leaf as for the Atlanta game sometime tomorrow.
Pro Bowl FB Mike Alstott's hamstring has been holding up just fine in practice and today, he participated in team drills. The team says the A-Train will play about 15 snaps on Friday against Atlanta.
Buccaneer Magazine has compiled a list of the Tampa Bay Buccaneers' injured players and their status on the eve of their flight to Atlanta to take on the Falcons.
OUT= 100 percent chance of not playing.
DOUBTFUL= 25 percent chance of playing.
QUESTIONABLE= 50 percent chance of playing.
PROBABLE= 75 percent chance of playing
The following players are OUT of Friday night's contest between the Buccaneers and Falcons: C Jeff Christy (left MCL sprain), G Russ Hochstein (left foot fracture) and DE Joe Tafoya.
The following Buccaneers' players are listed as QUESTIONABLE for Friday's game: WR Gerald Harris (left finger dislocation), DE Marcus Jones (right hamstring strain), DT Warren Sapp (left shoulder sprain) and DE Ellis Wyms (left foot sprain).
The following Bucs' players are PROBABLE for Friday's game: FB Mike Alstott (left hamstring strain), G Wilbert Brown (left elbow sprain), LB Marq Cerqua (left shoulder dislocation), K Martin Gramatica (left hip strain), LB Shelton Quarles (right groin strain) and P Mark Royals (right MCL sprain).
Subscribe to Buccaneer Magazine today by calling 1-800-881-BUCS(2827).


text:
EOJHL Regular Season Underway
View Single Post
Registered User
Join Date: Oct 2011
Posts: 394
vCash: 500
Renfrew bench was short for sure and they finished with 4 D. I am not sure if the game was ever in doubt as it seemed OTS played just well enough to win but could have played better if needed. For sure some OTS players tried to pad their stats last night but I would have thought the shot count was closer than 49-19. Couple of bad line calls lead directly to two OTS goals, one missed icing call in the first and a missed off side in the third but like I said, I don't think it really matter. The Renfrew goalie had a good game but the OTS back up is scary to watch. It seemed ever shot had a chance to go in but he stopped the puck when he needed to. I would think both Ottawa and Renfrew will be getting some more help on Dec 1 when Kanata and Pembroke get down to 23.
On another note...I think you see Allard back in Arnprior soon. Some of the new recruits are just not getting the job done. He will be on a very short leash, but they need him.
View Public Profile
KL14's albums
Find More Posts by KL14


text:
Search Profile Post Profile Log In Membership Plan Site Map
Awarded as Best Matrimonial Site...
Call Us : +91-11-47652233
Profile Created for Sister
32 years/ 5Ft. 3In. (160 cm)
Bachelor Degree
Banking & Finance
Lives in UAE, Dubai
Detailed profile
About MI-399563
I am Pooja basically from India but presently staying in UAE. br I belong to a Hindu Bhatia community. br br Education & work br --------------------- br br Done -B.com have 7 Years of International Banking Experience ... Currently She is Well Placed with a Swiss Private Bank in Dubai. br br Family br -------- br Have father who is Retired from Western Coal Fields. Mother is a Writer Singer and a Spiritual Teacher. Have 2 sister -1 is Married with 2 Kids. Brother-in-law Ex-military is Currently Employed with Mnc Based in Delhi br ... and Elder Sister stay along with me and working in a Private Banker with Mnc Bank Based in Dubai.
All Rights Reserved - MatrimonialsIndia.Com (2017)
Web Design by WeblinkIndia.NET


text: The M4 Sport in Danube Media sports channel that broadcasts after a two-week promotion 2015. July 18 was launched cent. This channel can be seen in the 16 priority sports events of Hungarian and international sporting events, outstanding presentation of Hungarian athletes, competitions, tournaments brokerage. This also includes self-produced programs. The M4 Sports roughly 10 hours of live provides daily sports coverage.


text:
Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
As each tradesman expresses interest in quoting on your job, we'll send you their profile and ratings to help you choose the right one for you.
After the work is complete, return to the site and rate your tradesman based on their quality of work, reliability and value for money.
Tree Surgeon
Added 30/08/2016
We have moved into a house with large garden and will need quite a lot of tree work. But immediately...
Searching for tradespeople
Tree Surgeon
Added 04/07/2016
Cut down one twisted willow tree
Searching for tradespeople
Tree Surgeon
Added 17/08/2014
I need about 5 trees felled and taken away and the stumps grounded .I also need some ground...
Searching for tradespeople
Managing your job is easier with the Rated People Homes App, download it today.


text:
With all the packing and everything else you need to remember to do before you go on holiday it's easy to overlook your mobile phone package.
Holidaymakers who don't plan ahead risk getting a nasty surprise when they return home to a painful bill.
The highest – and most hidden – costs apply to data roaming... using a smartphone to get on the internet or use apps abroad.
Unless you buy a special package before you go away it's always best to switch off data roaming on your phone.
And while you're at it disable your voicemail, or you'll pay every time someone leaves a phone message.
If you do have to go online, use email or make lots of calls, there are ways to cut your bill.
Our table shows the cost of using your mobile in the EU and US.
The good news is from July 1, the maximum that can be charged to make a mobile call from one EU country to another falls from 36p a minute to 28p, while the cost of sending a text will be cut from 11p to 8p.
Data charging will also be capped at 69p per megabyte (MB).
And customers have to be notified before the bill exceeds €50 (£40).
Providers are offering better deals too.
From July, O2 customers will be switched to a new Travel tariff allowing 25Mb of data per day for £1.99.
On calls, pay-monthly customers will pay 50p per call, then the minutes come off their contract.
It's possible to opt out and pay normal charges, but for many this will be a better deal.
Outside the EU there are no limits on what you can be charged.
For example, using a pay-monthly Vodafone mobile to call the UK from Turkey costs £1.65 a minute, and 35p to send a text.
Data costs £3 for the first MB and £15 for every MB afterwards.
Calls to and from the US are expensive too.
Phone network 3 charges £1.40 a minute to call the UK and 99p to receive.
With Orange data costs a steep £8 per MB.
One way to cut costs is to sign up to a bolt-on package, but they tend to be cost-effective only if you use your phone a lot.
Vodafone has a £3-a-day Eurotraveller bolt-on enabling pay-monthly customers use their UK price plan in Europe.
Orange have a £5.10 a month option giving 30 per cent off calls and texts outside the EU.
And 3 launched a Euro Internet Pass allowing customers to access internet for £5 a day.
An international SIM card is another option.
John Assiter, of GOSIM.com, says: "This lets you receive calls and SMS messages for free abroad as well as getting cheaper local calls and data. Data can start from 31p per MB."
Case study: "I came home to a £550 bill"
Alex Frith learned the hard way just how steep overseas mobile phone charges can be if you're not careful.
She returned from holiday in Thailand last year to find a £550 phone bill from Orange.
Alex, an account director, said: "When I got to Thailand it was raining so I used my phone to check the weather forecast to see when it was going to clear up.
"I also logged on to Facebook and watched YouTube clips. I had a few days before I met up with my boyfriend, so I probably used my phone more than I might have done otherwise.
"My bill is normally about £50 a month, so it was a real shock to get one for more than 10 times this – and I had to use my savings to pay it off. Even worse was the fact that it cost me more than my flight over there!"
Alex, 35, from Romford in Essex, now makes sure she turns off data roaming when she goes abroad.
She said: "Now I take my laptop when I go away and make use of free wi-fi rather than my phone."
The Moneymap's Steve Hodder's Q&A
Pound Notes: financial news in brief
Treasure Hunters: TV's Jamie Breese answers your questions on antiques and collectables


text:
Bring stylish, clean appeal to the bedroom with the bright white of this transitional king pillow sham. Hand-applique creates texture and visual interest for this appealing, casual bedding.
Our team has over 100 years combined experience with contemporary lighting.
Every EuroStyleLighting.com order is backed by our 100% Satisfaction Pledge.
Shipping and Delivery Information - For item EU9X497 if ordered by 1 pm Pacific Time


text:
We stay at the Hampton Inn whenever possible when we're in Morgantown (it books early for games). It's central to Morgantown and near a lot of good places to eat. Next door is an Applebees so you don't even need to drive. The hotel is clean, friendly, with nice beds. Newer TVs and a couch. The staff here is friendly and accomodating.
- Official Description (provided by the hotel):
- The Hampton Inn Morgantown is conveniently located next to the Evansdale Campus of WVU. We are just minutes away from sporting events, major medical facilities and entertainment venues. All 107 of our guest rooms are newly renovated and feature Cloud 9 Bedding, 32' inch flat screen televisions, workstations with roll around desks, ergonomic desk chairs and lap desks for your comfort. Our additional amenities include free wireless, high-speed internet access, complimentary breakfast daily 6:00AM-10:00AM, 24 hour business center, and a brand new fitness facility! ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Hilton Hotels Direct, Booking.com, Orbitz, Expedia, Priceline, Travelocity, Hotels.com, TripOnline SA, Hotwire, Cheap Tickets, Cancelon, HotelQuickly and getaroom.com so you can book your Hampton Inn Morgantown reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Hampton Inn Morgantown Hotel Morgantown
- Morgantown Hampton Inn


text:
Zombie Hankies Set of 3 by Cyanide Stitches on Etsy
Original Zombie Fashion for the cold and flu season. Who knows the next flu outbreak might be a zombie virus!!
Vaudeville is back and ready for your pocket this season!!Tags: bicycles, tissues, bikes, mustache, penny farthing, handle bar, hankies, Hankercheifs, hankyAdded 8 Years Ago from Etsy


text:
Press Release Historic Preservation Board Vacancy
Historic Preservation Board Vacancy - March 16, 2017, Anacortes WA – The City of Anacortes is seeking interested residents to fill a vacancy on the Anacortes Historic Preservation Board. The term of this position will run through December 31, 2019.
Applicants must reside in Anacortes and have demonstrated interest and competence in historic preservation. The major responsibility of the Historic Preservation Board is to identify and actively encourage the conservation of the City of Anacortes' historic assets by maintaining a register of historic places; raising community awareness of historic properties; and serving as a resource in matters of historic planning and preservation.
A background in history, architecture, archaeology, law, engineering or real estate is helpful. Must be able to attend regular monthly meetings and participate in preservation activities. The board consists of five members that meet the second Tuesday of each month at 4:00 pm at the Anacortes Museum Research Library, 1305 8th Street.
To apply please submit a Talent Bank Application (available on the city website http://www.cityofanacortes.org/Executive/Talent_Bank_App.pdf or at city hall) to:
Cherri Kahns
PO Box 547
904 6th Street
AnacortesWA 98221
Email: email@example.com


text:
- Latest
- Popular
- Blogs
- Authors
- Topics
- Our Blog
…, and don't think too hard. They do not have to be the 'right' books or great works of literature, just ones that have affected you in some way." So, which book finished at No. 1? Lada Adamic and Pinkesh Patel explained how they determined the top 100 books on the social network in a note on the Facebook Data Science page: To answer this question, we…


text:
Home >> Decorating Ideas >> Faux Finishing
Technical Data Sheets
Sign up for Hirshfield's
e-mail Newsletter and stay up to date on the latest in home decorating fashions.
The Minneapolis Institute of Fine Finishes, with the support of Hirshfield's, is excited to offer a variety of fine finishing opportunities for amateurs and professionals alike. These hands-on classes are taught by real professionals and range from basic painting to complex finishes and Venetian Plasters. Whether you are just beginning to paint or whether you have been painting for years, there's a hands-on class waiting for you. Register at any Hirshfield's store or visit plastercenter.com for more information.
Click one of the techniques below for how to instrutions
Modern Masters® Metallic Paint, available at Hirshfield's Decorating Centers, is an elegant alternative paint finish.
It's simple: just substitute ordinary latex paint with one of the beautiful shimmering metallic paints. You need neither artistic talent nor special skills to transform your project from ordinary to extraordinary.
Water Base/Non-Toxic
Clean Up with Soap and Water
Non-Tarnishing Metallic Colors
Easy Application
Brush, Roll or Spray
40 Shimmering Colors
Back to Top


text:
Students Feedback
Parul Institute of Medical Sciences & Research
- Parul Institute of Medical Sciences & Research
- Admissions
- Infrastructure
- People
- Committee
- Medical Education Unit
- Faculty Attendance List
- Awards & Achievements
- Hospital
- Affiliating University
- Citizen's Charter
- Academics
- Academic Meets
- Research Publication
- Research Projects
- Teaching Schedule
- Students Feedback
- Clinical Material
- Clinical Research & Ethics
- Dept. of Paramedical and Health Sciences
- Events
- Downloads
- Gallery
Latest News
Dr. P. Ambika Dhiman a Ph.D. Scholar of PU serves as Health Advisor to Hon. President of India
Congratulating Hon. President of India Shri Pranab Mukherjee for successful completion of his term We also congratulate our Ph.D scholar (Ayurved) Dr. P. Ambika Dhiman for serving as the Health Advisor to the Past President and the next elected Presidentmore info


text:
- List your item for as low as $30 - Advertise your item in both print and online
Create your ad
Browse: See our comprehensive list of earthmoving equipment specifications.
Compare: Try the compare button to see multiple specs side-by-side
Enquire: Hit the enquire button to talk to a dealer about your selection
Ron Horner has found another old-school excavator classic, this time a 1968 Kato 750G.+MORE
HASTINGS DEERING USED EQUIPMENTArcherfield, QLD, 4108
Trade Safely. Use our anti-scam guide to protect yourself.
Refurbished run and tested ready to go
Trade Price Trucks & Spares are one of Australia's largest Japanese Truck Wreckers specialising in second hand truck parts to suit Hino, Isuzu, UD, Fuso Mitsubishi most makes and models
We stock a large range of reco, refurbished or second hand Diesel engines & gearboxes
If its not in stock we may be able to find one or refurbish your one
Various Truck Bodies, Truck Cabs, Panels, Diff Centre's, Wheels & Tyres
With over 4000 Vehicles already been dismantled and trucks arriving daily chances are we will have or be able to find the part you are looking for
We also stock a large range of good quality wholesale Japanese Trucks
For further details please contact us with your requirements
The item you are attemping to add to compare is a different subtype to the items(s) in your list.
Only items of the same subtype may be compared.


text:
Web Development
The first step in designing a successful web site is to gather information. Many things need to be taken into consideration when we design the look and feel of your site, so we first ask a lot of questions to help us understand your business and your needs in a web site.
As the nature of your business you need to be prepared to tell us how your website should look and feel. Then we will make a prototype and we will follow the procedure step by step.


text:
California employers may violate an employment law almost daily, says the California Chamber of Commerce, "simply by trying to provide some flexibility for an employee, save money for the company or just be nice.
Here are the big 10 mistakes that are most likely to get them sued:
- Classify all employees as exempt, whether they are or not.
- Be nice to employees — let them work through lunch so they can take off early.
- Make everyone an "independent contractor" because having employees is too much trouble.
- Don't bother providing training about harassment and discrimination to managers and supervisors. They won't need the information.
- Let employees decide which hours and how many they want to work each day.
- Terminate any employee who takes a leave of absence, whatever the reason. It is too much trouble to administer leaves of absence, and who knows if the employee will return.
- Don't give employees their final check if they fail to return company property.
- Provide loans to employees and deduct the money from their paycheck each pay period.
- Use noncompete agreements to protect confidential information such as business secrets, customer lists and pricing information and prevent employees from working for the competition.
- Implement a "use it or lose it" vacation policy and avoid paying out all the money at termination.
"Keep in mind that this list will not apply to all employers as regulations and collective bargaining agreements may override these general rules," CalChamber says.
Click here for a CalChamber report that details why these actions get companies into hot water.
Other business stories…
- 18 favorite franchises to own
- 11 must-see movies for entrepreneurs
- 12 best franchises to buy in 2012
- 12 surprising things made in Orange County
- 11 worst businesses to start in 2012
- 11 hottest businesses to start in 2012
- Crocs dangerous for kids, lawsuit claims
- 10 brands youngsters like most
- Sexy photos anger O.C. fitness club members
- 8 foods Americans like best
- 11 highest profit businesses to start
- Calif. lost most jobs, firms in recession
- Local landscapes win beautification awards
- 27% of California firms 'a sinking ship'
- 10 consumer products that beat the recession
- Report: 99.7% of O.C. residents pay no use tax
- Poll: How much money does government waste?


text:
Our professionals provide hassle free documentation, customs brokering and inland clearance for the easy transportation of ordered consignments. These experts possess in depth of knowledge of custom regulations and have ample distribution networks with the officials in various countries.
15 C Chandigarh, Chandigarh - 141010, India
Container Truck,cargo Movers,freight Forwarder,personnel..
OUR SERVICES 1) OCEAN FREIGHT 2) AIR FREIGHT 3) RAIL FREIGHT 4) IN-HOUSE-HOUSE-CUSTOMS BROKERAGE 5) SURFACE TRANSPORTATION OCEAN FREIGHT We are one of the international freight forwarder and consolidator to offer our valuable customers Tailor-Made shipping programs. We offer end to end diversified range of import and export of ocean freight forwarding services including FCL and LCL consolidation services worldwide. W
Aairc Klobal Logistics
#16,bazzar Lane, Bazzar Road,saidapet, Tamil Nadu - 600015, India
We have a well trained and qualified team to handle all types of cargo and commodities, including heavy-lift and over dimensional as well as hazardous by sea and air.
Safeocean Logistics
Ludhiana, Punjab, India, Punjab - 141010, India
We provide custom custom website design. vardhmansoft, a competent web design & seo company from india well known to deliver custom website design services for all types of websites as per the requirement of customer in time and at affordable prices. Our service bouquet of custom website design solutions meet your needs & help your website to stand out in the crowd.
Rn-3, Mahesh Nagar, Near Electricity Complaint Office, Haryana - 133001, India
Post your Buy Requirement
We are one of the oldest and leading custom house agents with an experience of 121 years. We undertake custom clearance of all kinds of commodities for import and export. Our tailored service can take the headache out of every type of importation.
Tulsidas Khimji Pvt. Ltd.
46, Veer Nariman Road, Fort, Mumbai (bombay), Maharashtra - 400001, India
We are offering custom clearance service. Safeocean is your india agent offering complete import and export customs clearance.
Safeocean Express
Jagjit Complex, Jeewan Nagar, Punjab - 141010, India
We also undertake the custom clearances for our customers and our team of qualified professionals are there to handle documentation for all kind of commodities. We also provide warehouses transit warehouses as per the need and requirement of our customers on demand.
Anil Mantra Pvt Ltd
40, Chandigarh - 160101, India
Custom Application Development Services
We provide custom application development services just as you need for your business. Be it a web-based or LAN-based application, we are expert in both of them.
E-48,phase-8,mohali, Chandigarh , India
Custom Software Development Services
We create customized solutions for clients that greatly add to their productivity and efficiency Our services allow our customers to perform with maximum efficiency in minimal time.
8th Floor, Tdi Business Center, Sector 118 Mohali, Punjab, Chandigarh , India
Custom E Commerce Development Services
Are your current e-commerce portals not yielding the desired results? are they unable to attract customers and take your business to new heights? custom e-commerce development is the only solution which can reverse the trend.
Webguruz Technologies Private Limited
Plot No. 430 A, Industrial Area Phase-ii, Chandigarh - 160002, India
Income Tax, Service Tax, Sales Tax, Customs Tax, Corporate..
We provide services in Online accountancy and all the jobs related to Auditing , Accountancy and Tax returns.
S.k.bhasin And Associates
Sco 126-127,3rd Floor, Sector-34a, Chandigarh, 160022, Chandigarh , India
Freight Forwarding Agents, Custom Clearing Agents
We provide custom clearing and forwarding agent for benapole/ bangladesh.
Anand Shipping & Logistics
1st Floor, 13a, Kashinath Mullick Lane, West Bengal , India
Deals in Customs Clearing Services, Cargo Clearing Services, Freight Forwarding Services
Sun Ocean Shipping & Logistics Services
No.6,appa Samy Lane, Parys, Chennai-600001., Tamil Nadu - 600001, India
Post your Buy Requirement
Deals in Custom Clearing Services, Freight Forwarding Services
Afl Pvt. Ltd.
181, Indl Area - I, Chandigarh - 160002, India
Deals in Legal Services, Custom Clearing Services
Ess Kay Internationsal
Sco 371-73,sec 34achandigarh, Chandigarh - 160036, India
Deals in Import Services, Export Services, Warehousing Services, Custom Clearing Services
Varsha Shipping
51, Gma Building, Gujarat , India
Deals in Air Freight Forwarding, exim consultancy services, Custom Clearing Services, Transportation Services, Shipping Services, Freight Forwarding
Mars Shipping Agency
A/8, Ground Floor, Sai Sadguru Apts., Andheri-ghatkopar Link Road, Ghatkopar(west), Mumbai - 4000, Maharashtra - 400099, India
Deals in Custom Clearance Services, Transportation Services
Tricon Logistics (i) Pvt. Ltd
Suite # 13, Gr. Floor, Sai Commercial Centre, Govandi St. Rd., Govandi (east), Mumbai - 400 088, Maharashtra - 400088, India
Deals in Freight Forwarding Services, Custom Clearance Services
Wellway Logistics
Scf-27, 1st, Floor, Urban Estate, Punjab , India
Deals in Shipping Services, International Courier Services, Domestic Courier Services, Custom Clearance Services, Air Cargo Services, air freight shipping services
Gunina Group Gmbh
No. 4504/c , Sec 71 , Mohali, Chandigarh - 160036, India
Deals in import export code consultancy services, custom clearance consultancy services, focus license consultancy services, customs consultancy services, rcmc consulting services, Export & Import Consultants, advance license consultancy services
The Landmark Foreign Trade Consultancy
# 11/29a, Paris Nagar, Opp. Kims School,rnaerodrome Road, Coimbatore - 641014, Tamil Nadu - 641014, India
Deals in Warehousing Services, Transportation Services, Sea Freight Services, Customs Clearance Services, Air Freight Services
X, Tamil Nadu - xxxxxx, India
Deals in Logistics Service, Custom Clearance Services, Freight Forwarding Services
Core Logistic
Sco-90 91 92 2nd Floor Sector-8c Madhya Marg Chandigarh - 160018 India, Chandigarh , India
Deals in custom house agent services
Sunrise Freight Forwarders Pvt. Ltd.
Focal Point Phase V, Punjab , India
Custom Web Development Services
Deals in Search Engine Optimization Services, Website Design Services, e commerce web solutions, Application Development Services, custom web development services
Addiox Technologies
Sco: 179-180, Sec 8-c, Chandigarh - 160008, India
Custom Web Programming Services
Deals in Logo Designing Services, database development services, custom web programming services, Website Promotion Services, Search Engine Optimization Services, Website Designing Services, Website Maintenance Services
Thakar Infotech & Automations
#118, Bank Colony, Manimajra, Chandigarh - 160101, India
Custom Software Development Services
Deals in business process outsourcing services, Php Development, Data Conversion Services, IT Enabled Services, Custom Software Development Services, web hosting solutions, Data Entry Services, Mobile App Development
Matrid Technologies
#242, Sector : 15-a, Chandigarh - 160015, India
Deals in Website Development
Fatbit Technologies
F-8, Second Floor, Phase 8, Ind. Area, Sec 72, Mohali, Pb 140308, India, Chandigarh , India
Custom Website Development Services
Deals in Website Designing Services, flash websites development services, ecommerce website services, Custom Website Development Services, Graphic Designing Services
Digitech Software Solution
S.c.o. 839, Nac,manimajra, Chandigarh - 160101, India
Custom Dedicated Application Development
Deals in Mobile Application Development
Virtual Resource
Sco-11(iind Floor) , Sector-17e, Chandigarh - 160017, India
Deals in International Trade Services, Export Import Services, Customs Clearing
International Trade Consultants
Quiet Office Bldg. No. 12, Sector 35a, Chandigarh - 160036, India
Deals in Freight Forwarding Agents, customs clearing agents
Doff Shipping Pvt Ltd
405 Bps Plaza, Devidayal Road, Mulund Westrnnext To Best Bus Depot, Maharashtra - 400080, India


text:
Expand your existing wine collection, or start a new one with our Rohnert Park wine cellars. You can take advantage of our Rohnert Park complete remodeling services to get the house you have always wanted. Our Rohnert Park custom cabinetry and construction can help to add a modern look to your kitchen, while making it more functional. Make more room for yourself and organize your existing garage space with our Rohnert Park garage storage cabinets.
San Francisco, South San Francisco, Daily City, Marin, San Rafael, Sausalito, Tiburon, Corte Madera, Fairfax, Mill Valley, Bolinas, Stinson Beach areas, Sonoma and Napa Counties, Petaluma, Rohnert Park, Santa Rosa, Healdsburg, Napa, Calistoga, and Saint Helena.


text:
With First Tutors: Music you can search for a Nefyn cornet teacher. Cornet lessons are the perfect method to learn an entertaining new hobby, acquire a new skill and gain confidence with cornet. Use our service to find Nefyn cornet teachers and determine which best suits your needs so you can begin your cornet lessons in Nefyn straight away!
This is the best place to discover Nefyn cornet tutors in the UK, helping you discover Nefyn cornet lessons close to home and Nefyn teachers to assist with any instrument such as piano, violin or guitar. We can help you find Nefyn cornet lessons or a Nefyn cornet tutor to prepare for cornet exams and improve your cornet skills! Our search will order Nefyn cornet tutors near you by their tuition prices and distance from you.
If you are a cornet tutor and would like to offer cornet lessons in Nefyn or anywhere else across the UK, please register with First Tutors: Music or learn about us here. Becoming a Nefyn cornet teacher is a fantastic way to re-affirm your own skills through giving cornet tuition while helping others, all in the Nefyn area!


text:
Address: 23 N Derr Dr, Lewisburg, Union, Pennsylvania, 17837
Phone Number: 570-522-0229
Address: 23 N Derr Dr, Lewisburg, Union, Pennsylvania, 17837
Phone Number: 570-522-0229
Voting for a business allows it to have a running tally. If a business hasn't been positively voted for yet the displayed number will be 0. Negatively voting for a business will not make it fall below zero, however it will decrease the number of votes a business has if their tally is more than 0
The recommendation system is a way for customers of Amish Metal Roofs to let other people know whether they would recommend the business. You may recommend for or against using this business and your recommendations will affect this business's User Recommendation Score on Home Services Engine.
No public recommendations have been added for this business yet.
Can you see an error with the business details on this page? Please let us know so we can continue to provide you with the most accurate business results in the USA.


text:
Zed is a minor antagonist, Ned's twin brother, and an enemy of Dusty Crophopper. He is voiced by Gabriel Iglesias.
Out of the two brothers, Zed is the talkative one who often likes to bad-mouth Dusty, and not so bright, often getting hit by his boss Ripslinger, or Ned.
- Zed was the one who broke Dusty Crophopper's antenna.
- Zed is described as the rowdy & reckless one.
- He & Ned are known as the "Twin Turbos"
| Some or all of the information and/or categories on this page may have come from another site such as the Villains Wikia or TVTropes.org. This may include previous edits that are different than the current version. Changes to this page to provide original content are welcomed and encouraged, but this notice must remain on the page at all times.
To visit this page on the Villains Wikia, click here.


text:
Welcome to .
A website framework for serious business.
Create powerful business oriantated websites that you are gonna be proud of. Your business success is important to us.
Responsive & Retina
All of our sites are responsive and support all screen sizes including retina displayes. We know the importants of reaching the widest audiance.
Powerful Performance
Using a frame built from the bottom up, we have made every effort to keep your sites fast. Highest speed networks to the smartest code, makes things light and efficiant.
Truly Multi-Purpose
We focus on everything a business would need in one package. From ecommerce shopping baskets and inventory stock management to built in seo and security monitoring.
Lines of Codes
KBs of HTML Files
No. of Templates
Hours of Coding
Responsive Layout
Powerful Layouts with Responsive functionality that can be adapted to any screen size.
Retina Ready Graphics
Looking beautiful & ultra-sharp on Retina Displays with Retina Icons, Fonts & Images.
Powerful Performance
Optimized code that is completely customized and delivers unmatched performance.
HTML5 Video
Our hositng provides support for Native HTML5 Videos as well as video hosting sites like youtube.
User management
Subscription based management is built in. You can restrict and password protect any area of yours sites.
Endless Possibilities
We built this system from scratch. No frameworks where used. We can customise and build any requirement.
Light & Dark Color Schemes
Change your Website's Primary Scheme and layout instantly. A unique custom vaiable support has been built in to provide easy reusable html. No cloning required.
Our sister company specialises in website security, and protects all our hosting and backups.
AWESOME personal support.
Our support team are well spoken, friendly and very experienced. They cn help out via online call, or email.


text:
February 16, 2016
| Article
Nail tech and instructor Jeri Mallow shares tips to avoid burnout and promote a healthy attitude.
March 30, 2015
| Article
Julie Shepperly, a coach and trainer for beauty education provider Milady, shares what she's learned about moving forward and getting through those times when you just want to give up.
October 4, 2013
| Article
Editor Hannah Lee discusses the importance of taking time to recharge.
August 14, 2013
| Article
Check out these four ways to get your mojo back when you're singing the blues. Your body and mind will thank you, and you'll be able to enjoy your career in the nail industry for years to come.
February 1, 2009
| Article
Have you fallen short of achieving your goals? You can move forward, create clarity, and put realistic steps in place to bridge the gaps in your life.
December 1, 2008
| Article
As long as the sun rises in the East, they will be out there — bad days. We all have them. No, we can't make all the bad stuff go away but we can keep it from affecting our clients. Prepare in advance to avoid passing on all that negative energy, and your next bad day may pass without any negative toll on your business.
September 1, 2008
| Article
Looking for a positive way to get motivated and go to work each day? These readers share their secrets (and sense of purpose!) which will surely inspire you.
March 1, 2008
| Article
If you're feeling tired, apathetic, and cranky, you may need more than a good night's sleep; you could be suffering from job burnout. Early symptoms of burnout are so common, they are commonly overlooked. Learn to identify the symptoms of burnout and develop ways to protect yourself before you crash.
July 1, 2007
| Article
Combat boredom in the salon by varying your routine and trying new things from time to time. Whether you've got a little downtime or you simply need to kick-start a new routine, there are many ways to re-energize and refresh your surroundings in order to up the entertainment factor of your job.
Balancing work and home life can be a tough task in many professions, and nail techs are often susceptible to last minute appointments and closing time squeeze-ins. NAILS asked salon professionals if it is possible to maintain a healthy balance between work and personal life, and if so, how have they managed to do it.
February 1, 2007
| Article
Salons have always been centered around a beauty philosophy — but what happens when salons extend that outlook to include Mother Earth? As green salons pop up all over the country, find out what this trend means to you.
April 1, 2006
| Article
Don't let a full day at the salon wring the life out of you. Give yourself the care you need and you'll thrive even on the most challenging of days.
November 1, 2003
| Article
While death is an extreme result of stress-related illness, women are particularly susceptible to developing disease in response to stress--from depression and anxiety disorders to abdominal fat and colds.
While I am hardly an expert on balancing work and personal life, I know that since I've begun seeking a balance and creating time for family and friends, my life is richer.
November 1, 2002
| Article
In this month's Reader To Reader forum, we hear that sometimes learning more can be an inspiration.
CND Bootcamp 201745 photos
Beauty Changes Lives Legacy Awards Pays Tribute to Pivot Point Founder Leo Passage21 photos
Add on services are generally non-nail related services that can be added to a nail care service, and should not normally require additional...
Learn More
Is it normal that I'm in pain after doing a couple clients' nails?
How do you handle a client who constantly tries to correct you or tell you what to do?
What are reasonable terms for a non-compete contract?
How do I announce to my clients that I will be moving to a new salon?
The new products I'm using are adding time to each appointment. How do I adjust my schedule?
Featured Products & Promotions | Advertisement
How big is the U.S. nail business? $7.3 billion. What's the average service price for a manicure? Dig into our decades' deep research archives.
View All
VietSalon is a Vietnamese-language magazine and the sister publication to NAILS. Click the link below to sign up for a FREE one-year subscription.
Get a free preview issue and a Free Gift
Subscribe Today!


text:
Apple's eco-sanctimony
Uggghh. I love my iPhone, iMac, and Macbook Pro, but I can't stand the enviro-nitwittery of Apple management.
WaPo reports that Apple has withdrawn from the US Chamber of Commerce over its opposition to cap-and-tax:
Apple is pulling out of the U.S. Chamber of Commerce because of the organization's strident criticism of plans to reduce U.S. greenhouse-gas emissions, the computer giant said Monday.
In a letter to the Chamber's president, Apple Vice President Catherine Novelli wrote, "Apple supports regulating greenhouse gas emissions, and it is frustrating to find the Chamber at odds with us in this effort." As a result, Novelli said, "we have decided to resign our membership effective immediately."
The Chamber of Commerce, which says it represents more than 3 million businesses, has been one of the strongest critics of legislation aimed at reducing U.S. emissions.
Last week, the group's president, Thomas J. Donohue, said in a statement that his group supports "strong federal legislation" to protect the climate. But he said legislation passed by the House of Representatives — which would use a "cap and trade" system to lower the cost of reducing emissions — was flawed because it does not require other polluting countries to act and does too little to spur U.S. investment in green technologies.
A spokesman for the Chamber, Eric Wohlschlegel, wrote in an e-mail statement Monday: "While we'll continue to represent the broad majority of our membership on this goal, we recognize that there are some companies who stand to gain more than others with the current options on the table."
Steve Milloy's Green Hell blog points out that if Apple were really concerned about environmental health, it would leave China, not the US Chamber of Commerce:
So when will Apple pressure the Chinese government to adopt the Clean Air Act? Isn't actual air pollution in China much worse than the invisible, if not debatable/mythical, problem of U.S. CO2 emissions?
We doubt that Apple has any answers to those questions as Al I-need-cap-and-trade-to-become-the-first-carbon-billionaire Gore sits on its board of directors and, no doubt, cheered Apple's resignation from the U.S. Chamber.
Be sure to click on the link and see the compare and contrast photos of air quality at Apple's Cupertino CA office vs. Shenzhen, China, where iPhones are manufactured.
Eco-alarmist scientists determined to make 'climate' kill everybody one way or another
July 26, 2017 05:20 PM by Doug Powers
New Dem slogan a BIG hit with people who make money testing slogans (open thread)
July 23, 2017 08:21 AM by Doug Powers
Climate crisis getting so dire DiCaprio's *giving up private jets (*next Wed. only)
July 21, 2017 03:17 PM by Doug Powers
NPR & Bill Nye share 2-step climate change fix: You stop having kids, then die ASAP
July 20, 2017 07:54 AM by Doug Powers
Al Gore undaunted by inconvenient truth about Antarctic iceberg
July 14, 2017 02:05 PM by Doug Powers
Categories: Al Gore, Enviro-nitwits, global warming


text:
|Price incl. VAT
|Price excl. VAT
|Extended warranty + 1 year £10.72 Extended warranty + 1 year
|Return products within 60 days £6.70 Possibility to return products within 60 days
Inexpensive data repository that will solve the problem of storage of files once and for all. Ideal for the home.
Inexpensive data repository that will solve the problem of storage of files once and for all. Perfect for home use. The heart of the device is a Marvell Armada 370 processor with a frequency of 800 MHz. That coupled with 256 MB of memory provides plenty of power for relatively fast data transfer - an average read speed of 102 megabytes / s and write speed of 59 megabytes / sec. Furthermore, as such a processor equipped with a coprocessor. It increases the overall performance of the main CPU unit. You will feel a noticeable difference especially when loading thumbnails in a large number of photos or videos. Just some nice level of operating noise and surprisingly low power consumption.
Synology DiskStation DS216se is a great place to store your data. Not only do you save files easily, but also to them at any time, and above all easy to reach. The basic building block of a browser is the operating system of DiskStation Manager. This allows you to intuitively manage both server itself, but the stored data. Through a variety of tools and applications so you can peek into the vault of a wide range of compatible devices. Starting with a laptop and ending with a mobile phone or television. Moreover, thanks to Cloud can synchronize content between multiple devices. The operating system does not matter. Synology DiskStation DS216se supports both Windows and Mac or Linux. The same applies in the case of smartphones - compatible with both iOS and Android.
Information about the displayed capacity in Windows.
Specifications can be changed without notice. Images are for illustrative purposes only. Retail Price (MSRP) means the manufacturer's/supplier's suggested retail price.
Link to Manufacturer's Website: Enter link to the external review


text:
Warning: shuffle() expects parameter 1 to be array, string given in /home/alumni/www/alumni2.mighty-site.com/php_funcs.php on line 613
Student Rate:
$60 Initial Evaluation
$30 Office Visit
In addition, here is a list of some of the major health insurance providers that we service.
American Specialty Health
Anthem Blue Cross
Blue Cross
Blue Cross Blue Shield (out of state plans)
Blue Shield of California
Great West Health-Cigna
United Healthcare
Please note that insurance coverage varies depending on your particular policy and that not all insurance plans include chiropractic coverage. We would be happy to help you check on your insurance coverage, simply call our office and have your insurance information in hand (408) 244-1440


text:
Default start Page
I'm running an adult website based around WP, and my host has requested that I put up an entrance page prior to accessing the website it self. I have got an entrance page setup, but whenever I hit the title to go to the home page, the entrance screen pops up rather then the blog itself.
I changed the blog address to include index.php in it, yet all the URLs then have index.php in it. One other alternative I can think of is to insert a cookie onto the users computer to have them bypass the entrance page, but that won't work for people who don't allow cookies.
One other solution I can think of is to include a 'dummy' folder in the blog address.
If you have any alternative solution they would be much appreciated.
- The topic 'Default start Page' is closed to new replies.


text:
Many people tolerate a lot of distractions while doing their jobs and still get things done. Chris Forsberg is a veteran drifter who is very accomplished at his profession, but the question is: just how good? Forsberg's stripped car doesn't have any incoming texts, but there is a guy next to him putting a Taser to his chest.
Answering that age-old question, can you drift while being tazed?
- Chris Bruce
- Aug 21, 2014
TX car dealer sued for repeatedly tasering employee
- Brandon Turkus
- Aug 10, 2013
Tasers, while technically non-lethal devices, are not to be messed with. Just ask a police officer that's been tased in the name of training. Besides the crippling pain brought on by the electric shock, tasers can induce a number of heart conditions, up to and including heart attacks, while a 2008 study by Amnesty International determined that 334 deaths had been caused by tasers from 2001 to 2008 in the US alone. Like we said, it's not a toy.
Sharp-dressed man goes on vehicle-damaging rampage at CA Mazda dealer
- Chris Shunk
- Nov 24, 2011
Have you ever gotten so mad that you wanted to break something? For the vast majority of us, the urge passes without any property damage, but the same can't be said of Edward Roth. Fox 5 San Diego reports that the 22-year-old did his best Michael Douglas impression from the movie "Falling Down." The victim? Hines Mazda of Mission Valley, and Roth even managed to do the deed in a snazzy dress clothes. Surprisingly, the vandalism appears to be totally random – dealer officials say Roth has n
- 2018 Mustang GT does 0-60 in under 4 seconds
- How dealers are price-gouging Dodge Demon
- New Car Buying Guides
- 2018 Honda Accord is sleeker, more efficient
- Mercedes X-Class pickup truck revealed
- Find and compare 2017 Models


text:
Mail forwarding is a service that allows you to hire an address and have your mail forwarded to that mailing address. Capital Office`s mail forwarding service is like a mail box or a po box; in that you can use the City Road EC1 Central London address provided by Capital Office as your own.
Many people choose to have a mail forwarding address as provides many benefits when compared to using your own residential address as the main business correspondence address. One such benefit is that the mail forwarding address is a prestigious London business address, and your clients would be given the impression that your actual business is based there. Clients will not know it is a mail forwarding or mail box service as their is no indication of this on the address you use.
The address you use can be used on your own correspondence such as business cards, letter heads and on your website. Having a mail forwarding address instantly gives your business an established presence. Impressions are important and a mail forwarding service can help you achieve important goals.
Another benefit is security, a mail forwarding service from Capital Office is confidential. This means that your details are private and are not given out to anybody enquiring about your mail forwarding address. This is good for stopping unwanted callers turning up and wasting your time.
Once mail is received at your mail forwarding address, you can have it sent out to any location in the world. Most clients prefer to have the mail sent in batches – this can help reduce postage costs by sending the mail in one bundle as opposed to individually. However you the client can choose how you want it sent, each client has individual requirements, and we offer a flexible postage plan to suit your special needs.


text:
Sourdough bread will not rise
Can anyone help - my bread will not rise enough?
I regularly bake bread to a recipe originating from the island of Malta. It is a type of sourdough bread.
Can anyone help - my bread will not rise enough?
I regularly bake bread to a recipe originating from the island of Malta. It is a type of sourdough bread.
I have been attempting the no-knead method and have NOT been getting sufficient rise and very little oven spring. So, I have been trying to determine if my problem is being caused by the type of yeast I am using. Here are 3 items I have found that have me somewhat confused.
"USC researchers have extended the life span of baker's yeast 10-fold through a combination of genetic manipulation and caloric restriction, marking the greatest increase in prolonging life ever achieved in the laboratory."
I am a very new baker, low on experience (or quality baking tools), and perhaps too ambitious for my own good. As I wander the boards, I am faced with such a tremendous amount of information that I am not sure how to proceed.
This is my first post, and hope you can help. My mother's buttery egg bread recipe has disappeared, and I need it for next week. It consists of flour, eggs, milk, yeast, and salt. Makes a batter like dough, which rises in the fridge, with one punch down, then left overnight. It's dropped, soft and spongy, into loaf pans coated with melted butter, resulting in a crunchy crust. So, I know the outline, just need to recoup the details and proportions.
Sound familiar? The troops are hungry!
I am a new breadmaker from Toronto, Ontario. So far I have been using a Bread Making Machine, with mixed results. I think I am not using robust yeast, but I don't know where to find good, viable yeast. Does anyone have any suggestions? Best regards, Sommerpa
Hope anyone out there could help me out... I attended this 1 day baking seminar wherein the chef was making Pork Buns. He stressed out that in order for the bread to have a strong foundation inside for the pork fillings, you would have to add baking powder to the buns. How would this be true? I searched for pork bun recipes and found out that most of them do have baking powder included. What about adding baking powder to other breads other than pork buns... say, cinnamon rolls, swedish tea rings, interlaced breads, etc... Any answer would be a big help. Thank you!


text:
Download Flat Chris Young + Enter to Win a Signed Copy of 'It Must Be Christmas'
Ever dreamed of the Christmas morning miracle where your favorite celebrity is waiting for you down by the tree? Well, get ready because Chris Young is about to come home with you for the holidays!
Young has kickstarted his own 'Flat Stanley' campaign by encouraging fans to print out a miniature version of him to take him everywhere they go during the holiday season. From festivities with friends to eating Christmas dinner alongside your closest family members, Young is able to travel with his portable self to any occasion you can think of.
The country singer encourages fans to not only let him join along with wrapping presents and sipping hot cocoa next to the fire, but also to document the occasion each time he's hanging around with a quick post to any social media site with the hashtag #ItMustBeChristmas. The more, the merrier, as Young (and Santa) would say!
Some lucky fans who participate in the festive Flat Chris Young project may even get selected by the "I'm Comin' Over" singer himself to share on his own social channels online and get everyone in the holiday spirit.
Young made the yuletide season ever brighter with the release of his recent project, It Must Be Christmas, featuring both new and old Christmas carols on the tracklist. The album also features a slew of special guests, including Alan Jackson and Brad Paisley.
After you download your very own Flat Chris Young HERE, be sure to complete the form below to enter to win an autographed copy of Young's holiday album, It Must Be Christmas!


text:
Mark Stanley '78, New York City Ballet lighting designer and CFA professor at Boston University, discusses his design philosophy, his students, and his dual roles.
January 15, 2016
W&M physicists can take shifts monitoring a particle physics experiment from the comfort of Small Hall.
January 12, 2016
Curtis Pride '90 discusses William & Mary, his time as a professional athlete and his new role with Major League Baseball.


text:
Factory Reset Fortinet Box
This tutorial is to show you how to reset your Fortinet box back to factory settings. Every configuration is reset to default setting, and your Fortinet box is blank of policy or any settings that you created.
Firmware versions: 3.x and 4.x
Execute these commands in command line mode from your Java windows, or from CLI. If you need help about connecting to Fortinet using a serial cable, click here.


text:
Ask us anything!
If you have an account with us, please log in.
By creating an account with our store, you will be able to move through the checkout process faster, store multiple shipping addresses, view and track your orders in your account and more.
Availability: Out of stock
Regular Price:
Special Price
Have a Question?
Time to be a unicorn. This totally Tissue Jersey tank is soft and lightweight, featuring a unicorn graphic and clouds throughout. Scoop neck, open back and adjustable tie straps. A flowing, a-lined fit. 50% Cotton, 50% Polyester Model wears a size small.
About Us
In the Press
News & Events
Find a Retailer
E-Gift Cards
Size Guide
Privacy Policy
Contact Us
Shipping Info
Returns & Exchanges
Order Status
Shopping Bag