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Dendritic cells (DCs) connect innate and adaptive immunity, and are necessary for an efficient CD4+ and CD8+ T cell response after infection with
Infection of DCs with live Mtb (H37Ra or H37Rv) led to cell death. This cell death proceeded in a caspase-independent manner, and without nuclear fragmentation. In fact, substrate assays demonstrated that Mtb H37Ra-induced cell death progressed without the activation of the executioner caspases, 3/7. Although the death pathway was triggered after infection, the DCs successfully underwent maturation and produced a host-protective cytokine profile. Finally, dying infected DCs were permissive for Mtb H37Ra growth.
Human DCs undergo cell death after infection with live Mtb, in a manner that does not involve executioner caspases, and results in no mycobactericidal effect. Nonetheless, the DC maturation and cytokine profile observed suggests that the infected cells can still contribute to TB immunity.
Tuberculosis is responsible for 1.7 million deaths annually, and
The key role played by dendritic cells (DCs) in this successful host response has been well studied
We and others have shown how macrophage cell death follows infection with Mtb
Our results show that Mtb infection drives DC maturation and death. As we found in macrophages
Dendritic cells form an important link between the innate and the adaptive immune response, so their viability during infection may have consequences for the host. We prepared DCs from human blood as described in Methods. After 6 days' incubation, we reliably generated a population of DC-SIGN+ CD14- cells (Figure
Figure 1
Dendritic cells mature after they phagocytose
Dendritic cells mature after they phagocytose
Maturation was assessed in DCs infected with H37Ra. In controlled experiments, DCs were infected with live or dead Mtb H37Ra or at MOI 1for 24 h. Approximately 60% of cells had phagocytosed mycobacteria at this time point. The cells were washed to remove extracellular mycobacteria and either analysed or incubated for a further 24 or 48 h before analysis. DCs infected with live H37Ra displayed a mature phenotype, up-regulating CD83 and CD86 after 48 h infection with Mtb (Figure
To assess the relationship between intracellular infection and DC viability, we infected human monocyte-derived DCs with Mtb strains H37Ra and H37Rv. Viability of infected DCs (infected with 10 bacilli per cell) was assessed by PI exclusion and quantified on a GE IN Cell Analyzer 1000. Infection of DCs with either live strain was followed by cell death after 24-72 hours (Figures
Figure 2
Having established that reduced DC viability was dependent on infection with live mycobacteria, we then investigated the mechanism of cell death in H37Ra-infected DCs. We previously noted that macrophage cell death after Mtb infection results in DNA fragmentation. By ELISA, we could show that DNA fragmentation was also a feature of the DC response to viable Mtb H37Ra infection peaking at an MOI of 5 (Figure
Apoptosis results in nuclear condensation, pyknosis and, eventually, fragmentation of the nucleus into apoptotic bodies
Dendritic cell death after
Activation of caspases is considered to be essential for classical apoptosis
Figure 3
Dendritic cell death after
Dendritic cell death after
Our results so far indicated that H37Ra-infected DC death occurred with DNA fragmentation, but without nuclear karyorrhexis and was caspase-independent. Caspase-independent cell death can occur with or without caspase activation, depending on the mechanism of cell death
Secretion of cytokines by Mtb H37Ra-infected dendritic cells
Although macrophages and neutrophils die after Mtb infection, these dying and dead cells have been shown to play a role in host immune responses
Figure 4
Dendritic cells are permissive for growth of
Alveolar macrophages also die after Mtb infection and yet are capable of restricting the growth of Mtb
Figure 5
Dendritic cells are permissive for growth of
Dendritic cells are permissive for growth of
We investigated the impact of Mtb infection on the viability of human monocyte-derived dendritic cells. We found that DC death followed infection with both the H37Ra and H37Rv strains of Mtb, required viable bacilli, and could be detected at 24 hours co-incubation. The type of cell death was atypical of apoptosis, because it lacked nuclear fragmentation. Cell death due to infection with H37Ra was caspase-independent, although it did proceed with DNA fragmentation. Caspase activation was not detected by substrate assay analysis. Although this type of cell death did not interfere with earlier DC maturation events or cytokine release, it was not associated with any detectable mycobactericidal effect of DCs.
With regard to mycobactericidal effect, DC death differs from H37Ra-infected macrophage cell death, which can kill the invading parasite
It is perhaps not surprising that DCs failed to kill bacilli by themselves, without T cell help. Other consequences of macrophage cell death after Mtb infection have been described, such as better antigen presentation
In the present study we found that both the attenuated H37Ra and virulent H37Rv strains cause death of human DCs. The caspase-independent cell death we report in H37Ra-infected DCs appears to be neither apoptosis nor pyroptosis (both of which require caspase activity)
Caspases can have variable effects on the immunogenic potential of dying cells. Exposure or release of damage-associated molecular pattern molecules (DAMPs), such as calreticulin, high-mobility group box 1 (HMGB1) or heat-shock proteins, by dying cells is associated with immunogenic cell death. Calreticulin exposure has been shown to be of particular importance in the induction of immunogenic cell death
It is also possible that DC death could interfere with important DC functions such as migration to local lymph nodes for efficient antigen presentation. Others have shown that DC migration to local lymph nodes is impaired in Mtb infection
Mtb and the human immune system have co-evolved, so that one third of the global population has been colonised by this pathogen, yet the immune system is adequate at preventing disease 90% of the time
We have presented evidence that DCs undergo cell death after infection with Mtb
Inactivation of mycobacteria with streptomycin
Log-phase H37Ra were treated with streptomycin sulphate (Sigma, St. Louis, MO; 0.1 mg/ml) for 48 h prior to infection. Streptomycin was thoroughly washed from mycobacteria prior to DC infection.
Gamma-irradiated H37Rv
Obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH:
Cell Culture
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats of anonymous healthy donors (provided, with permission, from the Irish Blood Transfusion Service). The PPD status of donors was unknown. PBMCs were separated by density centrifugation on Lymphoprep (Axis-Shield, Oslo, Norway), washed and re-suspended in serum-free RPMI 1640 (Gibco, Invitrogen, Carlsbad, CA; for plastic adherence monocyte separation) or in PBS (Sigma) with 2% defined foetal bovine serum (FBS; HyClone, Thermo Fisher Scientific, Waltham, MA) and 1 mM EDTA (Sigma) (for immunomagnetic negative selection). Monocytes were isolated by plastic adherence, or by negative selection using the immunomagnetic negative selection EasySep Human Monocyte Enrichment Kit (STEMCELL Technologies, Vancouver, BC), as per manufacturer's instructions. For plastic adherence separation, PBMCs were incubated at 37°C for 2 h in serum-free RPMI. After incubation, unwanted cells were thoroughly washed from the adherent monocytes, which were then incubated in DC medium: RPMI supplemented with 10% defined FBS, 40 ng/ml recombinant human IL-4 and 50 ng/ml recombinant human GM-CSF (both ImmunoTools, Friesoythe, Germany). For immunomagnetic negative selection, PBMCs were incubated with magnetic particles coated with antibodies targeting unwanted cells (anti- CD2, CD3, CD16, CD19, CD20, CD56, CD66b, CD123 and glycophorin A). Labelled cells were magnetically separated and discarded, isolating the unlabelled monocytes. Monocytes were then incubated in DC medium. DCs were seeded on 24-, 48- or 96-well culture dishes at a density of 1 × 106 cells/ml and cultured for 6 days prior to infection with
On the day of infection, mycobacteria were centrifuged at 3,800 rpm for 10 min and re-suspended in RPMI 1640 containing 10% defined FBS. Clumps were dispersed by passing the bacterial suspension through a 25 gauge needle eight times, and the sample was centrifuged at 800 rpm for 3 min to remove any remaining clumps. To determine the amount of Mtb necessary to achieve the required MOI, a CrystalSpec nephelometer (BD Diagnostic Systems, Sparks, MD) was used to estimate bacterial numbers in
Propidium iodide staining for IN Cell Analyzer viability assessment
Viability was assessed using the propidium iodide (PI) exclusion method for plasma membrane integrity of cells, and the nuclei were counterstained with Hoechst. Cells were incubated with 10 μg of PI/ml, Hoechst 33342 (10 μg/ml), and Hoechst 33358 (10 μg/ml) for 30 min at room temperature. The number of PI-positive cells relative to the total number of nuclei per field was counted by automated fluorescence microscopy using the IN Cell Analyzer 1000 and IN Cell Investigator software (GE Healthcare, Pittsburgh, PA). Each condition was assayed in triplicate, and 8 fields were counted in each well. Staurosporine (Sigma) (1 μM, diluted in serum-free RPMI) was applied for 24 h as a positive control for cell death.
DNA fragmentation (cell death ELISA)
The cell death detection ELISAPLUS kit (Roche Applied Science, Mannheim, Germany) was used to quantify
Caspase Inhibition
The pan-caspase inhibitor, Q-VD-OPh (20 μM; Enzo Life Sciences AG, Lausen, Switzerland), was applied to DCs 4 h prior to infection with H37Ra and replenished every 24 h throughout the duration of infection
Caspase-Glo Assay
Caspase 3/7 activity was measured using the luminescent Caspase-Glo assay system (Promega, Madison, WI). DCs were cultured in 96-well plates and the assays were carried out in a total volume of 200 μl. After equilibration to room temperature, Caspase-Glo reagent was added to each well and gently mixed using a plate shaker at 300 rpm for 30 s. The plate was incubated at room temperature for 30 minutes and luminescence was then measured using a Victor2 plate reader.
Laser Scanning Confocal Microscopy
Following infection, DCs were fixed for 10 min (H37Ra) or 24 h (H37Rv) in 2% paraformaldehyde (Sigma), applied to glass slides and left to air dry overnight. The cells were then stained with modified auramine O stain for acid-fast bacteria and DC nuclei were counterstained with 10 μg/ml of Hoechst 33358. The slides were analysed using a Zeiss LSM 510 laser confocal microscope equipped with an Argon (488 nm excitation line; 510 nm emission detection) laser and a diode pulsed solid state laser (excitation 561 nm; emission 572 nm long pass filter) (Carl Zeiss MicroImaging GmbH, Oberkochen, Germany). Images were generated and viewed using LSM Image Browser (Carl Zeiss MicroImaging).
Flow Cytometry
Dendritic cell surface markers were analysed by flow cytometry on a CyAn ADP flow cytometer (Dako/Beckman Coulter). Dendritic cells were infected with live H37Ra, or streptomycin-killed H37Ra at MOI 1 for 24 or 48 h. As a positive control for maturation, uninfected DCs were treated with LPS (Sigma; 1 μg/ml) for 24 h prior to staining for flow cytometry. Cells were incubated with antibodies for 30 min and fixed with 2% paraformaldehyde for at least 1 h prior to flow cytometry.
The following antibodies were used: FITC mouse anti-human CD14, FITC mouse IgG2a, κ isotype control, PE mouse anti-human CD209 (DC-SIGN), PE mouse IgG2b κ isotype control, APC mouse anti-human CD83, APC mouse IgG1 κ isotype control, FITC mouse anti-human CD86, FITC mouse IgG1 κ isotype control (all BD Pharmingen, San Diego, CA).
Cells were gated on forward scatter and side scatter to exclude clumps and debris. DCs were CD14- and DC-SIGN+ (constituting approximately 90% of gated cells). Results were analysed using Summit software version 4.3 (Dako/Beckman Coulter).
Cytokine Analysis
Dendritic cells were infected with live H37Ra or streptomycin-killed H37Ra at MOI 1 for 24 or 48 h. LPS was applied for 24 h (Sigma; 1 μg/ml) as a positive control for DC maturation and cytokine secretion. Cytokine secretion was measured in cell-free supernatants by ELISA using the Meso Scale Discovery SECTOR Imager 2400 and the following assays: human IL-6 assay, and human Th1/Th2 10-cytokine multiplex assay, capable of detecting IFN-γ, IL-1β, IL-10, IL-12p70, IL-13, IL-2, IL-4, IL-5, IL-8 and TNF-α (Meso Scale Discovery, Gaithersburg, MD). IL-4 measurements were disregarded, as DCs were maintained in culture with exogenous IL-4, rendering it impossible to distinguish levels secreted by the cells themselves.
Colony forming units and BacT/ALERT 3D
Dendritic cells were harvested 24 h or 72 h after infection with
Statistical analysis
Results are expressed as means ± the standard errors of the mean (SEM). The data were analyzed with GraphPad Prism 5 software (GraphPad Software, Inc., La Jolla, CA) statistical software using by repeated measures ANOVA with Tukey's post test, or (where stated) by the Friedman test followed by Dunns multiple comparison test. A
Authors' contributions
RCMR performed the experiments and prepared the figures; MPOS performed the cytokine ELISAs; RCMR and MPOS analysed the data; MPOS and JK conceived of and designed the study; RCMR, MPOS and JK wrote the manuscript. All authors read and approved the final manuscript.
The authors wish to thank Dr Timothy Grant, Centre for Support and Analysis in Research (CSTAR) for providing advise on statistical analysis of the data. This work was supported by the Health Research Board of Ireland (HRB), Science Foundation Ireland (SFI-Immunology Cluster) and The Royal City of Dublin Hospital Trust. RCMR is a PhD candidate in the HRB-funded structured PhD programme in Molecular Medicine "From Genes to Function".
Fact Sheet No 104: Tuberculosis
Global Tuberculosis Control 2009: Epidemiology, Strategy, Financing
The role of dendritic cells in mycobacterium-induced granulomas
In vivo depletion of CD11c+ cells delays the CD4+ T cell response to
Dissemination of
A role for dendritic cells in the dissemination of mycobacterial infection
The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency
IRF8 mutations and human dendritic-cell immunodeficiency
A caspase-independent pathway mediates macrophage cell death in response to
Apoptosis of macrophages during pulmonary
Induction of cell death in human macrophages by a highly virulent Korean isolate of
Infection by
Apoptosis facilitates antigen presentation to T lymphocytes through MHC-I and CD1 in tuberculosis
Apoptotic vesicles crossprime CD8 T cells and protect against tuberculosis
Targeted delivery of amikacin into granuloma
Protective immunity against
Accelerated CD8+ T-cell memory and prime-boost response after dendritic-cell vaccination
Targeted induction of antigen expression within dendritic cells modulates antigen-specific immunity afforded by recombinant BCG
Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics
What do we mean when we write "senescence,""apoptosis,""necrosis," or "clearance of dying cells"?
Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009
Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties
Caspase-independent cell death
Apoptosis, but not necrosis, of infected monocytes is coupled with killing of intracellular bacillus Calmette-Guérin
H2O2 induces monocyte apoptosis and reduces viability of
Spontaneous or
Dendritic cell activation by sensing
Fas ligand-induced apoptosis of infected human macrophages reduces the viability of intracellular
CD43 controls the intracellular growth of
Rapid pathogen-induced apoptosis: a mechanism used by dendritic cells to limit intracellular replication of
IL-10 converts human dendritic cells into macrophage-like cells with increased antibacterial activity against virulent
Fate of
Constrained intracellular survival of
No life without death--apoptosis as prerequisite for T cell activation
Granulysin-dependent killing of intracellular and extracellular
CD4+ and CD8+ T cells kill intracellular
Mycobacterial lipopeptides elicit CD4+ CTLs in
Central memory Vγ9Vδ2 T lymphocytes primed and expanded by Bacillus Calmette-Guérin-infected dendritic cells kill mycobacterial-infected monocytes
The pyroptosome: a supramolecular assembly of ASC dimers mediating inflammatory cell death via caspase-1 activation
Immunogenic and tolerogenic cell death
Infection and apoptosis as a combined inflammatory trigger
Living on the edge: inhibition of host cell apoptosis by
Foamy macrophages within lung granulomas of mice infected with
Genetic basis of virulence attenuation revealed by comparative genomic analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv
Contrasting transcriptional responses of a virulent and an attenuated strain of Mycobacterium tuberculosis infecting macrophages
Control of M. tuberculosis ESAT-6 secretion and specific T cell recognition by PhoP
Expression of virulence of Mycobacterium tuberculosis within human monocytes: virulence correlates with intracellular growth and induction of tumor necrosis factor alpha but not with evasion of lymphocyte-dependent monocyte effector functions
Growth of virulent and avirulent Mycobacterium tuberculosis strains in human macrophages
New structural insights into the molecular deciphering of mycobacterial lipoglycan binding to C-type lectins: lipoarabinomannan glycoform characterization and quantification by capillary electrophoresis at the subnanomole level
Modulation of eukaryotic cell apoptosis by members of the bacterial order Actinomycetales
Human macrophages infected with a high burden of ESAT-6-expressing M. tuberculosis undergo caspase-1- and cathepsin B-independent necrosis
Calreticulin exposure dictates the immunogenicity of cancer cell death
Induction of immunological tolerance by apoptotic cells requires caspase-dependent oxidation of high-mobility group box-1 protein
Direct antigen presentation and gap junction mediated cross-presentation during apoptosis
Initiation of the adaptive immune response to
Interleukin 12p40 is required for dendritic cell migration and T cell priming after
Increased dendritic cell numbers impair protective immunity to intracellular bacteria despite augmenting antigen-specific CD8+ T lymphocyte responses
Bystander inhibition of dendritic cell differentiation by Mycobacterium tuberculosis-induced IL-10
Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5
Differential migration of human monocyte-derived dendritic cells after infection with prevalent clinical strains of
Src homology 3-interacting domain of Rv1917c of
Innate immune response to
Innate immune recognition of infected apoptotic cells directs TH17 cell differentiation
Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A
TNF-dependent BALB/c murine macrophage apoptosis following


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|Message view
|« Date » · « Thread »
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|Marcel Reutegger <marcel.reuteg...@gmx.net>
|Re: problem with index not being updated
|Fri, 13 Jan 2006 11:12:49 GMT
Brian Moseley wrote: > in fact, i make the repository available to the cosmo webapp as a > tomcat naming resource. this has already caused classloading issues > with this text filter, as cyrus daboo (the author) described in this > thread: <http://thread.gmane.org/gmane.comp.apache.jackrabbit.devel/4509> oh, I completely forgot to reply to this message, I apologize. the approach certainly works. as a quick recap here: the text filter is added to jackrabbit from within the web application that uses a different classloader than the one jackrabbit is using for its classes. I suggest to move your text filter implementation to the same class path where the jackrabbit classes are located. and then configure the text filter in repository.xml and workspace.xml. this will most probably solve the ThreadDeath issue you see. with your current setup, jackrabbit as a resource depends on a webapp classloader that is marked invalid at some point (cosmo reloads) and will never recover. moving the text filter class to jackrabbits classloader will avoid this dependency. > it may be that cyrus' TextFilterFactory approach causes the > classloading problem i'm seeing, but i can't say for sure, because to > be honest, classloading makes my head spin :) what do you think? yeah, classloading is not trivial stuff. I really suggest to move the text filter class. Or is there a specific reason why you want to keep the classes in the webapp? > and for the record, what is the "canonical" way to register a custom > filter with jackrabbit? the official way is to configure the filter in repository.xml and/or workspace.xml. See: http://issues.apache.org/jira/browse/JCR-265 regards marcel
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Přednášející: Jindřich Kolorenč (Fyzikální ústav AV ČR, v.v.i.)
Místo: Cukrovarnická 10, budova A, knihovna, 1. patro
Jazyk: anglicky
Pořadatelé: Oddělení tenkých vrstev a nanostruktur
I investigate tunneling of electrons from an STM tip to a substrate through a magnetic adatom by means of the Anderson impurity model. I employ an approximation analogous to the cotunneling theory [1] and concentrate on f-electron adatoms with large spin-orbital coupling. I compare the obtained inelastic electron tunneling spectra (IETS) with predictions of the spin model that has been very successful in the case of transition-metal adatoms [2]. When the spin model is applied to f electrons, the adatom spin S is replaced with its angular momentum J [3,4]. The spectra calculated in the two models differ, and I trace the difference to the exchange interaction between the adatom and the tunneling electrons. The bilinear (Heisenberg) exchange J.s, assumed in the spin model, implies that the magnetic quantum number m can only change by zero or plus/minus one for single-electron scatterings. The Anderson model indicates a larger number of allowed scattering channels. The less restrictive selection rule originates in the orbital exchange that is active for the orbital contribution to the magnetic moment of the adatom. This more general exchange influences also the lifetime of the magnetic states since they are destabilized, among other mechanisms, by the exchange with substrate electrons [4].
[1] F. Delgado and J. Fernandez-Rossier, Phys. Rev. B 84, 045439 (2011),
[2] M. Ternes, New. J. Phys. 17, 063016 (2015),
[3] T. Schuh et al., Phys. Rev. B 84, 104401 (2011),
[4] T. Miyamachi et al., Nature 503, 242 (2013).
Copyright © 2008-2014, Fyzikální ústav AV ČR, v. v. i.


text:
There is a catch to every big red can. Although buying 2 pounds of pre-ground coffee every couple of months may seem like an easy way to always have coffee on hand, a dirty secret lies hidden behind the facade of convenience: staleness.
Once roasted, coffee beans begin to release carbon dioxide and absorb oxygen. This oxygen initiates the breaking down process called oxidation, which is responsible for browning fruits and rusting metals. It's not exactly something you want to happen to your coffee.
Two weeks beyond roast is a general rule for determining whether coffee beans are beyond their apex. This may not seem like enough time to drink all that coffee you have. Here's the simple solution to that problem.
Buy less coffee more often.
It goes directly against the habit of buying big red cans, but it produces some fantastic results. Thanks to Alice.com, it can be even more convenient than the grocery store run.
Here are three terrific reasons why fresh coffee is always better.
Aromatic Oils Are Still Available
Roasted coffee beans break down slowly. The quickest things to go are the aromatic compounds in the coffee oils themselves. These oils, when available during brewing process, give life and flavor to a cup of coffee. When they have evaporated and dried up, the resulting cup is bland and uninteresting.
Many flavorists claim that roughly 80% of taste is actually a response to aromas exciting your sense of smell. With that in mind, don't you want very aromatic coffee?
You'll Taste Flavors Like Never Before
A cup of coffee made from beans out of the roaster seven days prior is a cup of coffee bursting with powerful, rich flavors. Think your pre-ground coffee is chocolaty? Just wait till you try those same beans freshly roasted and grinded just before brewing. The richness will make you rethink the phrase "it just tastes like coffee".
Somewhere Around the World, A Farmer Smiles
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Patient Comments: Bipolar Disorder (Mania) - Effective Treatments
What kinds of treatments have been effective for your bipolar disorder (mania)?
A Doctor's View on Catherine Zeta-Jones: A Case of Bipolar II Disorder
Read the Comment by Roxanne Dryden-Edwards, MD
Famous actress Catherine Zeta-Jones put a face on the mental illness of bipolar II disorder when she announced she was seeking treatment in 2011. Bipolar II disorder include symptoms like:
Main Article on Bipolar Disorder
Comment from: Bipolar Disorder, 55-64 Male (Patient) Published: March 04
Aged sixty-three, I am a patient of GAD (generalized anxiety disorder), major depression, panic disorder, anxiety disorder, migraine, phobia, insomnia, manic and bipolar disorder. For ten years, I have taken lorazepam, Librium, escitalopram, lots of alprazolam, Libotryp, quetiapine, sertraline, venlafaxine, mirtazapine, amitriptyline and nortriptyline. I am a diabetic and I also suffer from hyperdyslipidemia. During the period, I lacked minimum energy and remained confined to bed most of the time. Since the cause was exogenous more than endogenous and there was no change in the causes, I have now given up taking drugs. I only take sertraline 50mg, Ativan 2mg, mirtazapine 15mg and Librium 25 mg, one each before dinner. I feel better now.
Comment from: Mrytle, 65-74 Female (Patient) Published: December 19
I am 66 years old and became ill with bipolar in 1973. While raising my children I had many depressions and two psychotic breaks when I had to be hospitalized. However I was not given the diagnosis of bipolar until 1987. There were few effective medications available back then. One hospital I was in (1977) did not believe in giving medications at all. Since 2000 I have been fairly stable with minimum drugs of Depakote, Lamictal, Remeron and Ativan for sleep. I have had a lot of therapy by way of group and individual.
Comment from: benny81530, 35-44 Female (Patient) Published: October 08
I have bipolar disorder that has controlled my life for many years. I did many things that were risky and out of control. In the last year, I have gotten it under control. The main thing I did to change it was to quit taking Klonopin, as it only added to my manic episodes. I did this by myself. Soon after I quit taking it, I began to realize that I had to get my bipolar mania under control. My doctor gave me Geodon along with my other medications, and it has worked well. I now take lithium 300 mg, two times a day; trazadone 100 mg, two times a day; Geodon 80 mg, three times a day; and Effexor 150 mg, once a day. For more than a year now, I have been feeling real good with no manic episodes. I take my medications faithfully and continue to see my doctor every month. Whatever you do, don't stop taking your medication thinking that you don't need it.
Comment from: james, 35-44 Male (Patient) Published: June 04
I have been on Invega Sustenna medication for 6 months and feel suicidal and high anxiety. I have bipolar and am being treated for schizophrenia, nurses think another medication is going to make me better when the medication I am on now is wrong for me. There are too many side effects so I am going off my medicines, this will make me feel better. I like it better being in a mania than having these side effects, doctors do not care what they give you as long as you do not cause trouble. They don"t care if I feel awful, but I do, so I am stopping the medications.
Patient Comments
Viewers share their comments
Bipolar Disorder - Symptoms Question: What symptoms do you experience with your bipolar disorder?
Bipolar Disorder - Causes and Risks Question: Were you at risk for developing bipolar disorder? Please share your story.
Bipolar Disorder - Diagnosis Question: What tests were used to diagnose bipolar disorder in yourself or a relative?
Bipolar Disorder - Support Question: What forms of support have helped you or a relative who was diagnosed with bipolar disorder?
Bipolar Disorder - Types Question: Describe your type of bipolar disorder, along with your symptoms and treatment.
Patient Comments are not a substitute for professional medical advice, diagnosis, or treatment. Never delay or disregard seeking professional medical advice from your physician or other qualified health provider because of something you have read on MedicineNet. The opinions expressed in the comments section are of the author and the author alone. MedicineNet does not endorse any specific product, service or treatment.
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text:
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text:
BANGOR and MALVERN, Pa. — The parents of Michael Varney of Bangor and Sarah Berger of Malvern, Pa., are happy to announce the engagement of their children. Michael is the son of Timothy and Lori Varney of Bangor. Sarah is the daughter of Robert and Maryann Berger of Malvern, Pa.
Michael is a 2006 graduate of Bangor High School in Bangor and a 2010 graduate of Villanova University School of Business in Villanova, Pa., where he received a Bachelor of Science degree in business administration and finance with minors in Spanish and real estate. He is a commercial lines and surety producer for the Varney Agency. Sarah is a 2006 graduate of The Hill School in Pottstown, Pa., and a 2010 graduate of Villanova University School of Nursing in Villanova, Pa., where she received a Bachelor of Science degree in nursing. She is a registered nurse in the Main Line Health Care System at Lankenau Hospital in Wynnewood, Pa.
The couple is planning a Nov. 5, 2011, wedding at St. Thomas of Villanova Church at Villanova University in Villanova, Pa. The reception will be held at the Philadelphia Country Club in Gladwyne, Pa.


text:
Apologies if the answer is out there already ...
We have ~ 200 users in Windows AD envrionment but with external hosted exchange. Is there a quick way of adding all active AD accounts with end-user rights access to Spiceworks Portal ?
This topic was created during version 5.0.
The latest version is 7.5.00101.
4 Replies
Jan 21, 2011 at 4:35 UTC
use the AD sync. Other option is to not use AD and users just need to enter their email address (no password).
Jan 24, 2011 at 3:54 UTC
We already had AD Sync functioning. However as we use an external third party hosted exchange email service, our AD accounts did not have anything other than user name fields populated. In particular no email addresses had been populated.
Putting email addresses into the AD user details resolved the probalem.
Jun 17, 2011 at 8:07 UTC
1st Post
Hi John,
did the spiceworks database update the email field automatically after modifing the user in AD?
I'm doing the same work (add email info in AD for all users) but almost all changes are not updated into spiceworks!
AD sync is set to auto, every 15 min.
Thanks in advance,
Jun 29, 2011 at 9:34 UTC
I am having the same problem. Spiceworks doesn't appear to be updating the user list, no matter how often you have it check in the settings. Has anyone found an update to this?
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Para-Plus Translations, Inc.
Toll Free: 800-558-3011
THE AMERICAN DREAM: When Para-Plus Translations, Inc. founder and president Sonia Santiago arrived in the U.S. from Puerto Rico at age 14, she faced a new school and community without any English language skills. She quickly learned the language, then practiced in the mirror to get rid of her accent. She went on to create a multi-service translation and interpretation agency with service capabilities in over 100 languages, which has grown every year for 34 years.
PASSING THE TORCH: Second-generation owners Robert and Carlos Santiago took a circuitous route to ownership and governance. Working outside the company, both acquired business skills that ultimately allowed Para-Plus to grow and the organizational structure to change. "Developing, maintaining and adhering to a governance system within the family firm has been very important to us," Robert Santiago says.
Quick Tip "In business, business comes first. In the workplace, decisions must be objective and, as best as possible, not personal."
PERFECTING PROCESSES: Robert Santiago says the second generation's challenge was to build on past success, continuing to provide service excellence and manage client relationships during a time of internal transition and economic downturn. They sought to keep the core values of a family-based organization while bringing the business to a level that was more consistent with the demands of a technology-based generation.
BUILDING A LEGACY: Robert Santiago says he wants to create a great work environment where everyone treats each other with respect and individuals feel they are effectively contributing to the success of the organization. "To help key employees learn to make day-to-day decisions in the absence of ownership, you force them to make tough decisions on a trial basis and run independently," he says.


text: With that, I'll turn the call over to Tony.Tony Somma Thanks, Bruce. Good morning, everyone. Earnings per share for the quarter were $0.38 compared with $0.47 last year. For the quarter, gross margin increased $10 million or 3% due mostly to higher prices. Weather in the quarter, as measured by cooling degree days, was almost the same as last year. Turning to the expense side, O&M for the quarter increased about $12 million or 13% excluding a $4 million increase in SPP transmission cost, most of which had a favorable revenue offset. It should be noted, last year we reversed a liability associated with assets we sold in the 1990s, thus reducing 2010 O&M by $5 million. Current year increases include the following: $4 million of Wolf Creek, half of which was for regulatory compliance and the balance due to increased expenses for this year's refueling and maintenance outage; $2 million on our distribution system for tree-trimming where we're seeing value and a more sophisticated approach to optimize electrical reliability with more targeted expenditures, this program will be a component of our upcoming rate case as we like to expand it; and $2 million increase on property taxes which was also offset with higher revenues. Partially offsetting the increases were the $2 million reduction in storm amortization expense, something that will continue through the remainder of the year. SG&A expenses increased $8 million due primarily to higher legal fees related to the arbitration of two former executives and the amortization of deferred energy efficiency program cost with the latter also having a corresponding revenue offset. Last, depreciation expense increased $4 million due largely to planned additions for environmental and transmission projects completed last year. Now, turning to our financing plans for the year, through June we settled 3.1 million shares priced last year on a forward contract, receiving about $66 million. We still have 9.7 million shares priced last year, reflecting about $226 million of available equity that we plan on selling this quarter or early next. Beyond this, we don't have plans to sell additional equity this year.


text:
Nebraska Methodist College of Nursing & Allied Health Entertainment News
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text: Wouldn't it be great to earn money with just making photos with your smartphone? It's a lot easier than you think! Using these apps you can make $250 for a picture. All you need is an iPhone or Android device and a photographic eye. Here are the apps that will turn your creativity into cash:


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Does My Shirt Smell Like Chloroform To You T-Shirt, Hoodie, Tank Top
$23.95• FREE SHIPPING when you order 2 or more shirts. Use code "FREESHIP" in checkout. (Details)
• Womens sizes run small - see size guide below.
Product Details
You can order this Does My Shirt Smell Like Chloroform To You t-shirt on several different sizes, colors, and styles of shirts including short sleeve shirts, hoodies, and tank tops.
Each shirt is digitally printed when ordered, and shipped from Northern California.


text:
A 19-year-old Garfield Heights resident was sentenced by Judge Tammy O'Brien in Summit County Court of Common Pleas April 6 in connection with the October armed robbery at Panera Bread on Darrow Road.
Alexis M. Olenik Barrett pleaded guilty to robbery Feb. 27. A firearm specification was dismissed.
She was sentenced to two years community control, 60 days home incarceration and received an 18 month suspended jail sentence. She also was ordered to pay $2,953.83 in restitution to Panera, jointly with the other two defendants Corey D. Johnson, 26, and D'Jaye Quinae Redrick, 18, both of Cleveland.
Redrick is scheduled to be sentenced June 8. He pleaded guilty to robbery, a third-degree felony, Feb. 27. A firearm specification was dismissed.
Johnson pleaded guilty March 14 to one count of aggravated robbery with a firearm specification; a repeat violent offender specification and having weapons while under disability charge were dropped.
Johnson was sentenced that day to seven years in jail, including a three-year mandatory sentence for possession of a firearm; and four years for the aggravated robbery. The three-year sentence is to be served consecutively with the four-year sentence; Johnson will be credited for the 137 days already served. Johnson was given five years probation after he is finished with his jail sentence.
According to Twinsburg police reports, officers responded to Panera Oct. 3 at 9:21 p.m. for an armed robbery. No injuries were reported in the robbery.
Email: firstname.lastname@example.com
Phone: 330-541-9423
Twitter: @twinsburgohio


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Women's Impact Network call for proposals
The Women's Impact Network of New Hanover County, a women's giving circle formed in partnership with the North Carolina Community Foundation, has announced its 2014 grants cycle with a focus on health and wellness needs of New Hanover County residents.
The Network invites area non-profits to submit a letter of inquiry explaining what a program might be able to do with a one-time grant of up to $32,000. Network members will use the letter of inquiry to identify organizations that most closely match the funding interests of the Women's Impact Network.
2014 Letter of Inquiry Procedures
Deadline: Letters of Inquiry must be emailed on or before Friday, April 4, 2014.
Important: Submitting a Letter of Inquiry does not guarantee formal consideration for funding. Letters of Inquiry only provide us with preliminary information. After reviewing the Letters of Inquiry and conducting other research, the Women's Impact Network Grants Committee will invite selected organizations to complete a full grant proposal. If an agency is selected to submit a formal application, they will be notified by April 30th, and full applications will be due to the North Carolina Community Foundation by Sunday, June 15, 2014.
Letter of Inquiry Form: Please complete the LOI form, which serves as your Letter of Inquiry. Organizations may submit only one Letter of Inquiry.
Funding Priorities: The intent of the Women's Impact Network is to award grants that have the potential for high impact, resulting in significant positive change in the community. Women's Impact Network members are especially interested in:
- Projects that address health and wellness needs of New Hanover County residents
- Creative and non-traditional approaches to meet these needs.
- Proposals that offer opportunities to collaborate and foster partnerships with more than one local organization, as well as leverage other funding sources.
- Projects that demonstrate the ability to achieve measurable results and have a high probability of successful continuation beyond the grant period and/or are replicable.
- Organizations must be located and provide services in New Hanover County.
- Applicants must qualify as tax-exempt under Section 501(c)3 of the Internal Revenue Code or be classified as a unit of government.
- Organizations must not discriminate on the basis of age, race, national origin, ethnicity, gender, physical ability, sexual orientation, political affiliation or religious belief nor be a religious organization that serves exclusively sectarian purposes.
Click here for the 2014 Letter of Inquiry invitation.
Click here for the 2014 Letter of Inquiry proposal form (required for submission).
For more information about the Women's Impact Network of New Hanover County, please contact Patricia Lawler, Regional Associate, North Carolina Community Foundation, at (910) 509-7256 or firstname.lastname@example.com.


text:
Disney Princess 6 Bin Storage 471DIR
The attractive 6 Bin Storage unit will make tidying up a pleasure rather than a chore for your little ones. With its strong MDF frame and 6 fabric storage drawers it is ideal for keeping clothes, toys and games tidy.
Note: Delivery within 48 working hours.
Features & Specifications:
-Disney Princess storage unit - practical and convenient for storing toys, book and games
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-Assembled size (h)60 x (w)63.5 x (d)30 cm
-Weight: 4.3 kg
In the Box:
-1 x left panel
-1 x right panel
-9 x batons
-6 x fabric storage bins
-1 x set of screws
-1 x instruction sheet


text:
Australia vs Sri Lanka 3rd Final live Score Match Highlights CB Tri-Series 2012 at Adelaide on 8 March 2012. Australia cricket team will face Sri lanka in 3rd Final of Commonwealth Bank Series (CB- Tri Series) 2012 at Adelaide.this match Aus vs SL 3rd Final live cricket game most interesting because Series of Final leveled by 1-1 in Australia and Sri Lanka.Australia team well play in 1st Final and beat Sl but other hand 2nd Final Sri lanka play very well and beat Aus by 8 Wickets. this time and in this series both teams Australia and Sri Lanka game very care fully and they provide good entertainment for their fans to enjoy full match live stream and enjoy full highlights results at the end of game. so we provide Australia vs Sri Lanka 3rd Final live score match highlights results online game videos at here to enjoy full match with ball to ball live commentary and Scorecard.
Mahela Jayawardene and Tillakaratne Dilshan took full toll of a wayward Australia as Sri Lanka forced a third triangular series final in Adelaide. Chasing 272, the visitors galloped to the target with 5.4 overs to spare, benefiting greatly from a rollicking start when the first three overs reaped 30 runs.
Dilshan alternated between the brazen and the cheeky, crashing 10 fours and also being struck on the helmet when he attempted his patented Dil-scoop. The captain Jayawardene was more cultured, but outpaced his partner in a princely innings that would have ended in the second over if not for a Clint McKay no-ball.
Such indiscipline summed up Australia's predicament, having struck a horrid patch of form in the field midway through the first final in Brisbane and then being unable to rise above it in Adelaide. The captain Michael Clarke is also in the unenviable position of carrying a left hamstring problem perilously close to the start of the West Indies tour, due to begin almost immediately after the third final.
Australia vs Sri Lanka-Aus vs SL 3rd Final live Score Online Updates 2012
Australia vs Sri Lanka 3rd Final live Score Match Highlights Videos Results Timings
Australia vs Sri Lanka 3rd Final Live 2012.
CB Tri-Series 2012.
Date:- 08 March 2012- Time:- 12:00
Venue:- at Adelaide.
Watch Live Stream TV:- Sky Sports HD, Ten Sports,NEO, ESPN,Ten Sports HD, Star Cricket,Star Cricket HD.
When the triangular made a comeback this Australian summer, there was trepidation around it. After all, it was going to involve 12 ODIs before the best-of-three finals series between sides that were used to playing each other. Familiarity between India and Sri Lanka has borne boredom for a long time now, and on top of that Australia had a strong mental hold over India after a summer full of Test-bashing.
Australia and Sri Lanka 3rd Final Squads Team Players List:
Australia squad:
MJ Clarke*, SR Watson, GJ Bailey, DT Christian, XJ Doherty, PJ Forrest, BW Hilfenhaus, DJ Hussey, MEK Hussey, B Lee, NM Lyon, CJ McKay, JL Pattinson, MS Wade†, DA Warner.
Sri Lanka squad:
DPMD Jayawardene*, AD Mathews, LD Chandimal†, TM Dilshan, HMRKB Herath, CK Kapugedera, KMDN Kulasekara, MF Maharoof, SL Malinga, NLTC Perera, KTGD Prasad, KC Sangakkara†, SMSM Senanayake, WU Tharanga, HDRL Thirimanne, UWMBCA Welegedara.
Australia vs Sri Lanka 3rd Final live Score Match Highlights CB Tri-Series 2012 at Adelaide:
Australia vs Sri Lanka 3rd Final live Score Match Highlights CB Tri-Series 2012 at Adelaide to enjoy full game and enjoy score card results online.all fans enjoy Aus vs SL 3rd final live game live cricket score online results and highlights videos here with match time scorecard full.stay here for enjoy full match latest updates.


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Initially, I didn't like this product at all but it grew on me. I hated the texture of it, how I had to add water to the mixture before applying it on my face, and how messy it was to use! But after a week of putting up with the messiness, I noticed that my skin tone improved and I wasn't as oily if I used this product the day before.
I believe I bought the 100 g pot, which was $14. I'm used to buying drug store cleansers, so this was pricier than I was used to. But since I tried it before, I knew that it would be worth the price!
The texture of the cleanser was something I have not seen before. I took a closeup, because I'm not too sure how to describe it. It is a bit moist and flaky, and a tad bit grainy.
These reviews are the subjective opinions of ChickAdvisor members and not of ChickAdvisor Inc.


text:
Guild Name: Eorzea Corps
Leader: Destinova
Server: Undetermined
Type: All play styles
Website: http://www.eorzeacorps.com
Recruiting: Active mature members
Our sight is set on the horizon. With a willingness to help new
and old members we will be focused on progression of skills,
knowledge, mission's, and gear in a way that rewards the
helpful and deters the disdain.
Edited, Mar 18th 2010 10:11pm by EorzeaCorps
Edited, Mar 19th 2010 12:19pm by Pikko Lock Thread:


text:
Andy Wolf, Love is the new, second line of the Austrian eyewear brand Andy Wolf. It just launched together with a third line, Andy Wolf, Soul. All products are manufactured and handmade in Hartberg, Styria, where the label has been founded in 2006. Since then the eyewear company raised in international esteem. Andy Wolf, Love embodies the label's passion for new design and exciting color combinations. The first campaign is a tribute to the 70s (what else ;)).
The collection includes sunglasses as well as optical glasses for women and men. Among the models are mirrored sunglasses, cat eye diva stylings and round nerd glasses.


text:
Love baking and fancy a little competition? This October, we will be hosting our first pumpkin pie bake-off! As a member of our local community, you are invited to bring in your most delicious, homemade pumpkin pie. The amazing Cotswold Chef™, Rob Rees, will be judging all entered pies alongside pies from some of our very own team members. The winner will win a pair of tickets to London's spectacular Spirit of Christmas Fair – one of the classiest shows in the UK and a great way to get you ready for the holidays!
All are welcome to participate – mums, dads, kids, professionals and amateurs alike!
Pies should be dropped off on 30 October, between 10.30 am and 12.00 pm, to our Acorn Cook Centre here at the Cheltenham store.
Any further questions? Please contact Customer Services on 01242 542950.


text:
Nigerian students in Russia having took to the streets of Moscow on Monday, to protest over a 12 month unpaid stipends at the Nigerian embassy.
The students who are beneficiaries of Bilateral Education agreement with Russia, a scholarship initiative of the Nigerian government managed by the Ministry of Education, which provides funding for undergraduate and postgraduate studies in selected countries, accuse the federal government of failing to keep their promise in paying their stipends, which has been left unpaid since October 2015.
Meanwhile, one of the protesting students in his tweets claimed that they were left in the cold to freeze for one hour thirty minutes before the embassy officials came to address them, only for them to be told that they didn't come along with a written petition to the Federal ministry of Education.
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text:
Jane Williams B.Sc., M.A., M.Sc. (Certified Clinical Animal Behaviourist), ABTC listed
Jane has many years' experience as an animal trainer and behaviourist. She is a full member of the Association of Pet Behaviour Counsellors (APBC) and of the Association of Pet Dog Trainers (01005) (APDT). Jane is a Certified Clinical Animal Behaviourist (ASAB). She qualified as an animal behaviourist, having completed both the Post Graduate Diploma in Companion Animal Behaviour Counselling and a research M.Sc. in the same subject area at the University of Southampton.
Company:Athenae Ltd.
Courses by this Tutor
3 March 201810 - 4£90 inc. home-made lunch, biscuits & cakes etc.


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| This article or section about a Quest is incomplete or in error. You can help AoC Wiki out by editing it.
|The Smuggler
|Main Category
|Tortage daytime
|City of Tortage
|Quest Starts At
| Smuggler Note
|Minimum Level
| 16
| • Talk to the Smuggler
I. Finding the Smuggler Edit
Objective Edit
- Talk to the Smuggler
Journal Entry Edit
A note on the wall reads:
""Strom's thugs will be here any minute, so I better leave if I want to see another day. If you still need the goods smuggled out of Tortage, bring them to me in my house in the Poor District. Make sure no one follows you."
I should locate the smuggler if I want to leave Tortage early.


text:
100 Favorite Dishes: The Alferd Packer Salad at MAD Greens
The Alfred Packer salad in a to-go container.
Linnea Covington
No. 73: The Alferd Packer Salad at MAD Greens
While many eateries tout their use of seasonal ingredients, it's not often that you can find a dish that truly represents what's coming out of Colorado farms right now — especially at a quick-service restaurant. Never fear: With the special Alferd Packer salad, MAD Greens has created a bowl of pure Colorado love — but it's only available for a short time. The oddly named salad is made with Palisade peaches from the Clark Family Orchard, roasted Olathe sweet corn coming from Ahlberg Farm, and crisp green beans straight from the vines at Petrocco Farms in Brighton. Also gracing the bed of romaine are dots of tangy goat feta from Jumpin' Good Goat Dairy in Buena Vista and citrus-grilled chicken. They're all tied together with a light balsamic vinaigrette, which heightens the tangy-sweet combo of peaches and goat cheese.
Give thanks for this bountiful, healthy lunch to chef Dan Long, one of the founders of MAD Greens and the company's chief culinary officer. Through mid-September, you can get this salad at any of the fifteen Colorado locations. While you're there, check out such other tasty options as the Siam I Am salad with kale, soy beans, quinoa and spinach; the Annie Oakley panini, made with slow-roasted chicken, bacon, swiss and avocado; or the fresh-baked chocolate chip cookies. The first MAD Greens opened in 2004 in Centennial and over the past decade, the company has opened stores across the state and in Arizona, Texas and Utah.
Convicted cannibal Alferd Packer would have surely stuck to Colorado veggies if there had been a MAD Greens nearby when he was making his dining decisions. And, as a bonus, $1 for every Alferd Packer sold will be donated to Denver Urban Gardens.
Hungry for more? All the dishes in our 2016 countdown are linked below.
No. 100: Masala Beef at Biju's Little Curry Shop
No. 99: Bacon Shrimp and Grits at Bacon Social House
No. 98: Porchetta and Kimchi Sandwich at Brider
No. 97: Squab-and-Sunchoke Tortellini at Abejas
No. 96: Housemade Burrata at Viand
No, 95: Schweinshaxe at Rhein Haus
No. 94 Kimchi Jeon at Seoul BBQ
No. 93: Zuppa Pomodoro at Parisi Italian Market & Deli
No. 92: The Ocean Salad at Sushi-Rama
No. 91: The Signature Bowl from Churn & Burn
No. 90: Green Chorizo at Los Compadres
No. 89: The Diavola Calzone at Pino's Neighborhood Pizzeria
No. 88: Biscuits and Green Chile at the Noshery
No. 87: Chirashi at Izu Sushi
No. 86: Brisket and Ribs at GQue Championship BBQ
No. 85: The Tornado at the Chowder Room
No. 84: Korean Fried Chicken Wings at Yong Gung
No. 83: A Meatball Hero at Lil' Ricci's New York Pizzeria
No. 82: Shakshuka at Stowaway Coffee + Kitchen
No. 81: Late-Night Ramen at Brazen
No. 80: Potato Tacos at El Tejado
No. 79: Corned Beef Heart at theWay Back
No. 78: The Fried Bologna Sandwich at Snarfburger
No. 77: Sweet Corn Mezzaluna at Bar Dough
No. 76: Duck Confit at Table 6
No. 75: Croque Monsieur at Fire
No. 74: Tahini Cookies at the Preservery
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Running Time: 56 minutes
Released: 09/2008
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Resist Hoodie
$ 45.00
Throw your hood up, throw your freedom lovin' hood up... on this 'RESIST' hooded sweatshirt! Utilitarian and durable, this hoodie is perfect for everyone taking part in the resistance.
- Hand screen printed in California
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Measurements are taken of the front of the shirt. For example, a Men's Small says Chest = 18", which means it measures 18" from left to right of the front of the shirt. The full shirt circumference = 36" (front and back combined).


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On September 4th 2016, I made the tough decision to leave Wichita, Kansas for Greenville, South Carolina. I had spent five years in Wichita as an athlete and Graduate assistant so leaving what had essentially become my home for the last five years was no easy decision. On the other hand, I had a lot of important people that believed in me and wanted me to chase my dreams in becoming the best possible athlete I could be. Therefore, making the move to Greenville and joining Furman Elite was definitively the right move for me. I did notice straight away that I was going to be spoiled in Greenville compared to Wichita due to some great weather. There is literally no wind in Greenville and 25mph winds is the norm in Wichita!
The first few weeks in Furman Elite was made up of a lot of firsts. I learned that the community of Greenville was very invested in running which is probably helped by some awesome Furman University cross country programs, the swamp rabbit trails that run for miles into downtown, and some Elite track clubs in the area. DEXA scans, blood tests, hurdle mobility, plyometrics, pool routine, long runs at DuPont State Forest, and training at altitude were some of many new things we had to get use to. The team and I also got to help out at both of Furman Universities home cross country meets which was a great and fun way to meet both the men and women's teams.
Now that 2017 is here, I am excited to finally race consistently. I opened up my season on January 21st with a 4.01.86 mile which is the fastest I have ever opened up. Who knows, maybe Coach Gary will stop saying I've the biggest thighs on the running circuit now! On a serious note though, I would definitely contribute my best opener ever to having incredible training partners at Furman Elite and training for a month in Flagstaff, Arizona. I am currently training through this week before pacing the men's 3k at the JDL Fast Track meet on February 4th, and then I will race a 3k in Boston were I hope to achieve the standard for the European Indoor Championships. Exciting times are ahead for sure.
** I just want to say a huge thank you to all the support we receive from Asics, boosters, Performance Therapy, and Mike Shride. Without you guys, none of us would be able to do what we do and love.


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|1,506 sq. ft.
|Property type
|Minimum Stay
|3 - 7 nights
This luxury residence is part of a newly realized chalet village built in the typical Austrian style. The combination of wood, stone, and a fantastic unobstructed view of the mountain scenery makes a stay unforgettable.This tastefully decorated chalet is located at the foot of the protected National Park 'Hohe Tauern' in which the eternal snowy peaks of the Großvernediger and Großglockner are located.In the winter period the popular ski areas Wildkogel, Königsleiten and Zillertal can be reached easily within a few minutes by car or with the ski bus for free.Chalet Grünwald is divided into two floors, each with a separate entrance and are also connected by an internal staircase. The two floors can also be rented independantly from one another.
With an area of 140 sqm chalet has the following features:Ground floor (Grünwald B):- Entrance hall- 1 bedroom with a double bed Smart TV, en-suite bathroom (sink, shower, hairdryer)- 1 bedroom with a double bed and Smart TV- 1 bathroom (sink, toilet, shower, hairdryer)- Washer and dryer
- 1 Studio - useable as bedroom:
- Open kitchen with dining area- Cozy seating area with Smart TV and (electric) Fireplace- Sofa bed for 2 persons- Access to the patio and garden through sliding glass doors
- Access to the upper floor via an internal staircase with a lockable door
Upper floor (Grünwald A):- Entrance with direct access to large living room and cloakroom- 1 bedroom with a double bed- En-suite bathroom with bath, wash basin and a bio sauna with built-in infrared emitter- 1 separate toilet- Open kitchen with dining area- Cozy seating area with Smart TV and (electric) Fireplace- Sofa bed for 2 persons- Access to the balcony through large sliding glass doors- Access to the ground floor via an internal staircase with a lockable doorThe interior is equipped with ceramic floor tiles with wood finish, fully equipped kitchen and modern sanitary facilities.There is one parking space in the underground garage and additional parking available outside.There is a storage room for ski equipment with boot dryer and bikes.Important information arrival:In winter we recommend to take snow chains with you as the chalets are situated on a hill and the access road is steep.The details shown in the property description could possibly have changed something, so please contact our team for more information.
Additional costs:
(Refundable Security Deposit): €700Final Cleaning: €190Bed-linen + Towels: €15 per Person/setLocal Tax: €1,10 per Day p.Person (over the age of 15 years)
Gute Betreuung, super Austattung. Wir waren sehr zufrieden.
*Plus compulsory or optional charges based on actual occupancy or consumption. The payment has to be made in the currency offered by the property manager, before use of conversion tool. Payments in other currencies have to be negotiated beforehand with the property manager. The currency conversion tool is provided for your convenience only and is non-binding.


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Forent Energy Ltd. is pleased to announce the appointment of Mr. Brad R. Perry, CMA, as Chief Financial Officer of the Company. Mr. Perry will be responsible for the Company's finance, accounting, administration and risk management activities.
Mr. Perry is a Certified Management Accountant with a Bachelor of Commerce degree from Saint Mary's University in Halifax, Nova Scotia. He has 23+ years of oil and gas experience including 16+ years as the CFO of several junior oil and gas exploration and development companies listed on the TSX and TSX Venture Exchanges. Notably, Corridor Resources Ltd. in Halifax and, most recently, Tuscany Energy Ltd. in Calgary. Mr. Perry brings significant governance, strategy, reporting, analysis and M&A experience to Forent.
Forent Energy
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Roy Miller Flint Ridge Dovetail
- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Advertising
- Source: eBay
- Sold Date: Mar 22, 2009
- Channel: Auction House
This is a beautiful modern knapped flint ridge dovetail made by Master Knapper Roy Miller. It measures 1 1/2" wide x 5 1/8" long. It is signed Roy Miller 2000. Do not miss out on this beauty !Buyer pays $6.50 shipping and handling. International buyers pay exact shipping charges. We will combine shipping on multiple lots. Payment is expected within 10 days of close of auction.


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DEJAH THORIS Original Comic Art - Mike Grell
- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Books, Paper & Magazines
- Source: eBay
- Sold Date: Nov 25, 2006
- Channel: Auction House
Dejah Thoris Original Cover art Prelim by Mike Grell. Done in pencil on a 11x17 Illustration paper.
All my items are stored in a temp controlled, smoke free environment. If you have any questions, please email me.
U.S. Winning bidder pays $9 Priority shipping/ins
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Good morning all,
It occurred to me last night when I couldn't sleep that Henry James, that formidable intellect and author of the masterful The Turn of the Screw, also committed a heinous sin against literature: in The Golden Bowl he named a character Fanny Assingham.
Fanny Assingham!
From my way of thinking, there is no clearer sign that you and I should be gentle with ourselves and laugh off our occasional mistakes. At least we didn't name a character Fanny Assingham.
I wondered, now jolted from sleep by this vision of Fanny, if it were the worst name in all of literature. Thomas Pynchon has come up with some real groaners, but he knows it and does it for his own purposes and delights. There are plenty of bad names out there. Most are probably just flat-out forgettable.
But are there any worse than Fanny Assingham?
If you've got a nominee, please make your case below in the comments.


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text: There is no market which is more fast moving, nor more ephemeral, than that of social media. Vulnerable to the vagaries of fashion and personal taste, the big players in this field are not known for their stability, but rather for their fortunes fluctuating rapidly, and experiencing considerable skepticism about their market value. Thus, the original social network website - so notorious that they made a movie about it - Facebook Inc ( FB), was considered grossly overvalued by many analysts when it first floated, and quickly tumbled in value. There were two fundamental problems which Facebook faced.


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Students of Liechtenstein studies need not worry about paying for over-priced textbooks anymore – renting textbooks is an option! Liechtenstein textbooks – for example Let's Go: The Budget Guide to Germany, Austria & Switzerland, 1993/Including Liechtenstein and Eastern Germany by [Geoff Rodkey], and Frommer's Switzerland (Frommer's Complete Guides) by [Darwin Porter, Danforth Prince] – are often some of the most frequently updated books by Publishing companies. The field of Liechtenstein is constantly changing, and new editions can come yearly and at a huge cost to students who continually buy new books. So why buy when you can rent cheap textbooks? There are plenty of Liechtenstein books to rent – BookRenter has of them all!
Used book sales can be a cheap option as well, but often can be more expensive than textbook rentals. However, there are very few return policies with used books, and especially when taking Liechtenstein classes, buying a used book can be dangerous and costly.
Let's Go: The Budget Guide to Germany, Austria & Switzerland, 1993/Including Liechtenstein and Eastern GermanyList Price:$16.95
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List Price:$16.95
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Ayoub hints at possible changes in Al Ahly squad against Al Wahda
Al Ahly's assistant manager, Ahmed Ayoub, stated that Al Wahda clash, in...
Mohamed Salah featured as Torino managed their first home win over Roma since 1990, on matchday six of the 2016/17 Serie A season.
Roma went into the match after an overall good start to the Serie A season, sitting in third in the league with 10 points from their opening five games, and on the back of a 4-0 win over newly promoted Crotone.
Torino were less fortunate, and aside from a 5-1 drubbing of Bologna they had failed to win a match so far, and went into the match following two goalless draws against Empoli and Pescara.
Roma created some early chances but failed to find Edin Dzeko on two occasions, the second of which was a weak Salah cross.
Nainggolan's shot from distance came close but ultimately failed to hit the target.
Roma were made to pay moments later, when Andrea Belotti added to his already impressive tally of four goals from five games with a header at the near post.
The chances kept falling to Dzeko, but the score remained 1-0 until the half-time whistle blew.
Early in the second half, Torino won a penalty as Belotti was brought down by ex-Torino defender Bruno Peres, and Iago Falque – on loan from Roma – scored the penalty against his parent club.
Almost immediately afterwards, Diego Perotti was brought down in the box by De Silvestri, and club legend Francesco Totti sent Joe Hart the wrong way as his penalty found the top left corner of the net.
Torino doubled their advantage through Falque again, thanks to his deflected shot over Szczesny after the ball broke to him from Belotti's heavy touch.
Although Roma are still in third place in Serie A, they will likely be overtaken if Milan or Inter win their games in hand.


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Category Archives: Press
SocialGO launches sgv2.com
SocialGO has recently opened signups for sgv2.com – the home of SocialGO Version 2! The new platform will be running alongside the existing SocialGO.com platform for the next few weeks as we collect feedback and introduce new features for subscribers. … Continue reading
SocialGO's Royal Encounter
SocialGO Co-Founder Dominic Wheatley recently spoke at Variety's Venture Capital & New Media Summit – a conference exploring how the investment community is driving technology innovation. Our Dom was lucky enough to rub shoulders with the Duke and Duchess of … Continue reading
An introduction to conversation marketing
Conversation marketing Manchester University Sunday, 27 February 2011 The term "conversation marketing" first came about in the book Join the Conversation by Joseph Jaffe. Businesses conducting conversations with their customers is far from new but Jaffe wanted to look at … Continue reading
SocialGO Raises $2.2m + Version 2 Screenshots
We are pleased to announce that SocialGO PLC has successfully raised $2.2m to fund the business through the launch of Version 2 and beyond. The whole team is excited about the year ahead as we see the new platform come … Continue reading
SocialGO targets the Facebook Generation
French politician Dominique de Villepin is using SocialGo to mobilise supporters and communicate with them, though you wouldn't know it is an off-the-peg solution. The basic paid-for set up is impressive, the design is slick, and the dashboard easy and … Continue reading
SocialGO – A Part of East London's Media Revolution
This article from the Economist website explains the UK government's hopes for the emerging digitial media startups in the East end of London. "The entrepreneurs in Silicon Roundabout stress the unique draw of the east of the city, with its … Continue reading
SocialGO Helps UK Government Launch Major Policy Initiative
SocialGO Co-Founders Steve Hardman and Alex Halliday recently played key roles in the launch of the British Government's major new initiative, the East London Tech City. Steve Hardman gave a speech on the challenges facing software startups in the United … Continue reading
The Future of Social Media and Social Networking
Here is a very interesting presentation from the recent World Social Networking Congress that gives some great insights into the future of social media and social networking. Simply click on the image above to download. Enjoy…….


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To travel through Wales is to journey through thousands of years of history, culture and tradition, which never ceases to amaze! As you make your way you can count on quality rest and refreshment at our six historic Inns. Businesses that are as much a part of the story of Wales as the stunning landscape in which they sit.
Wine and dine in a delightful old thatched cottage that was once ancient stables, or stop for a tasty bite and a refreshing ale in a building once frequented by aristocrats. We're renowned in the area for our delightful beer gardens and some of our Inns even offer tranquil ponds to captivate the imagination. At every one you can pull up a chair by a roaring log fire in a pub that can be as popular with the spirit world as it is with our worldly guests.
Find out more and download a colour or back and white copy of our complete collection guide for South Wales.
From the coast in the south to the lush mountains and valleys to the north, Wales has a great story to tell - and our Vintage Inns are there to keep you good company on the way!


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It's been 12 long years since Hrithik Roshan and Kareena Kapoor Khan starred together in a movie, they came very close, when Karan Johar signed them for Shuddhi but unfortunately the project fell through.
Hrithik backed out of the movie due to his surgery and other prior work commitments and once he left, Kareena too ditched her close buddy, Karan Johar as she says and we quote, "The idea of doing Shuddhi was to work with Hrithik (Roshan). Once he decided to opt out, it didn't work for me."
Salim Feels Salman Khan Is Not Fit To Be Anybody's Role Model
So Kareena clearly backed out of the project for Hrithik, turns out that the stars will soon be in a love triangle. You heard it right! If reports are to be believed Hrithik Roshan and Kareena Kapoor Khan will soon share screen space in Tanuja Chandra's upcoming movie which is said to be a love triangle between an army officer, pilot and a woman.
Earlier Shahid Kapoor and Ranbir Kapoor were said to be cast in the lead roles, but that did not work out and the project was shelved for some time.
Now, Sharman Joshi has been said to be cast as one of the leads, so the project may soon start. Fans can't wait to see Kareena and Hrithik Roshan together on screen again.
Check out their old pictures together...
Mujhse Dosti Karoge
This picture of Kareena and Hrithik was taken on the sets of Mujhse Dosti Karoge.
Kareena Kapoor and Hrithik Roshan's chemistry was so hot that people believed them to be a real couple.


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[BUG] Program requests save even when saved (589725)
Expand Messages
- I submitted this at Sourceforge.net as bug number 589725
If I open any character in PC Gen, the program will, without fail, demand
that I save the character because it's changed. This happens whether or
not I change anything. It even happens if I hit the "Save All" menu
-- Rob Bowell
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"I think he wanted to be able to look them in the eye and see how he was doing," Georgia head coach Mark Richt said. "You could learn a lot by looking in guy's eyes about where he's at mentally. You may not necessarily want to pull a guy if he's struggling. But you want to know that he's struggling so you don't put too much on him. You might run some plays that might take some pressure off him."
Richt, who was the coordinator for years at Florida State, said he was always more comfortable in the press box.
"When I came to Georgia I realized I was really a visual learner type person," he said. "I could be in the box and really see the whole thing. I thought make good decisions based on what I was saying. Whereas on the ground level you can't see the big picture."
Injury updates
Receiver Logan Gray's impressive camp has hit a stumbling block. The converted quarterback is out 7-10 days after spraining his ankle in Tuesday's practice.
"Not a high ankle sprain, which was good, but it did well up pretty good," Richt said. "Any of those guys getting hurt's a shame, but Logan he's been practicing so well, and had his best practice (on Tuesday) and then he ends up spraining his ankle right at the end."
Inside linebacker Akeem Dent (toe surgery) could see live action in practice next week, but Richt said the staff is "100 percent certain" the defensive co-captain will be ready for practice the week of the opener.
Richt also expects G Cordy Glenn (mononucleosis), center Ben Jones (knee) and ILB Mike Gilliard (hamstring) to be able to have a full week of practice before the opener.
Safety Nick Williams also got hurt early in Thursday's practice, apparently with something upper-body related. Richt wasn't sure what injury it was said Williams was going full-tilt at the end of practice.
Diving trash talk
Richt's high dive on Monday – wearing street clothes – made ESPN SportCenter's top 10 plays. He was told Thursday that Mississippi State coach Dan Mullen rated Richt's dive a 5.7.
"Our own diving coach said he'd give me about a 6.5 in a real meet," Richt said. "Mullen gave me a 5.7? All right, I might get him on that. Call him up right now."
Game prep begins
More than two weeks before the opener, the Bulldogs have broken into some scout team drills to get ready for Louisiana-Lafayette. That's earlier than normal, due to the team starting practice earlier to make up for only having one two-a-day.
"I'm not sure whoever's going to be on the scout team today is always gonna be on the scout team the rest of the year," Richt said. "But it's always a tough day for some guys, hoping maybe not to be on the scout team right off the bat. But that's a very important position for us."


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Search our database to find out how your school fared.
Okaloosa County maintained the "A" grade it's held since 2014, while Escambia County earned a "C" for the second year in a row.
Five of the six schools who received a "D" past year improved one letter grade.
"We worked so hard". Elementary schools saw the largest increase in "A" schools with 30 percent, up from 21 percent a year ago, state officials said. "If we didn't improve, I don't know what else we could have done".
The state said the percentage of schools that earned an "A" or "B" increased to 57 percent.
If Florida schools had a year book, Miami-Dade would hands down win "most improved". "We will continue to implement stronger leadership and instructional support structures for these schools, which will result in a continual increase in student achievement".
The number of schools receiving an A doubled to six from previous year.
Sarasota County Schools has earned another A grade for the 2016-2017 school year from the Florida Department of Education, and is one of only two districts in the state to have earned an A every year since grading began in 20014.
More than 40% of the Martin County schools received an A and just as many received a B and just three schools were rated C, in 2017. Boynton Beach High went from a D to a C. Olympic Heights in Boca Raton moved from a B to an A. It has become clear to our community that each school's progress can not be represented by a single assigned letter grade.
Of Duval's two F grades, only one, Ramona Boulevard Elementary, is a traditional school.
"I am proud that more than two-thirds of the schools that were being monitored through the school improvement program improved to a C or better".
Palm Beach County School District held on to its B rating, as did each of Florida's other six large urban districts, including Broward and Miami-Dade counties.
In Broward, nearly 36 percent of schools serving populations in which at least 7 in 10 students are poor improved their letter grade.
Fifteen Lake schools increased at least one letter grade, four schools dropped a grade and 26 schools remained the same, according to the education department's annual report.
More than 70 schools in the three counties made the state's list of the lowest performing elementary schools, based on English language skills and learning gains.


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MISSES AND WOMEN'S ONE-PIECE DRESS IN HALF SIZES: The front bodice of this flattering dress features three horizontal tucks. Rows of gathers give softness to front shoulder edge and front and back waistline. View 1: Each tuck features two notches. A small notched collar forms a V shaped neckline. The three quarter length sleeves are cuffed. View 2 has a square front neckline and flared sleeves.
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