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The new Paleo/Primal thread - please reintroduce yourself, newcomers welcome!(906 Posts)
Wow, we got to 1000 messages!
Welcome back from the previous thread and welcome to all new!
I have been Paleo since September last year, I started as an ex-vegetarian/vegan, I wanted to give up gluten and I had been doing 5:2/IF, which lead me to Paleo/primal. I am 40, I have 2 kids age 8 and 6. I have no major health issues, but I would like it to stay that way. I have bad skin, which has improved since starting Paleo and my dandruff has disappeared. I have gained some weight since starting Paleo, but I was not overweight before, although I would like to get back on my pre Paleo weight - I just love Paleo mayonnaise too much ;) I still practice IF and I lift weights 2-3 times per week. My family is also Paleo.
I have just come back from a camping weekend where I was not strict Paleo as I was camping with other families and I just didn't want to spend my entire weekend telling my kids "no, you cant have it". I do think that Paleo camping is entirely possible, think tinned fish, nuts, sausages, eggs etc.
I don't know if grains can damage your gut wall but they feed candida. When candida switches to it's fungal form (rather than yeast) it grows roots which pierce the gut wall and lead to leaky gut. It is then more like a parasite and will make you very unwell indeed!
The candida infestation I had was affecting my thyroid function which in turn was messing with my hormones big time. I was totally staggered to learn the impact diet makes to overall health (I feel silly saying that but nobody teaches you these things.) Everything is connected!
No I feel like I have healed my gut and like you say, my tastebuds are reset and I can enjoy food as it should be! I also think grains are evil
Thanks for the info about thyroids. I'm going to have a big read of the perfect health diet to see what they say about it.
FoxPass - from what you say about the gluten/thyroid link, do you think it would be worth taking supplements of magnesium and Vit D, as well as the selenium and iodine? I already use epsom salts when I have a bath, but don't really take any supplements at the moment. I also intend to supplement with a good quality omega 3 fish oil.
I'm coming to the conclusion that grains are evil too!
peonies I take a spoonful of coconut oil every day as it is very good for supporting thyroid function. I also eat 3 brazil nuts for selenium and use a magnesium spray daily.
I'm not sure what else directly supports thyroid function but I also take a multivitamin and omega 3 oils daily.
Thanks Fox, I already cook with coconut oil quite a lot so hopefully I'm getting that anyway. Will do more research into whether other supplements would be helpful.
Rawcoconut - do you think you could clarify your views on grains, please?
Actually I don't disagree with you. I think that properly prepared grains a la Nourishing Traditions book are probably ok but no one does that anymore....
Paeonies - I'm fairly certain that I was subclinically hypothyroid before I came to all this. (A number of years of preg and b/feeding had been the final straw for my overly taxed body). My diet was probably ok ish - lots of fruit and veg but I ate grains. Anyway, I'm now taking magnesium, vit d, vit k and iodine and vit c daily, b vits and selenium once/twice a week, as well as being primal plus safe carbs. The perfect health diet folk don't recommend either a multivitamin or fish oils - it's all there on the website, it's fascinating reading.
I do know that taking iodine for hypothyroid issues is very controversial. The theory seems to be that it helps only if selenium and magnesium levels are ok too. But you don't want to take too much selenium either.... Also, there is also a theory that too low carb can exacerbate hypothyroidism in the long run. Minimal carb definitely did not agree with me so I eat white rice and white potato.
My DH is slightly horrified by my diet (aghast that I eat eggs in butter most days etc) so I have agreed to get my cholesterol checked. I'm slightly scared about it - what if my levels are sky high !!
Ps fox pass - I agree with you about feeling staggered by it all. I think I have heard "eat whole grain" and "low fat" so long that you don't question any other way.
I couldn't believe how crap I was feeling 3 months ago. Chronic sinusitis, brain fog, exhaustion, always cold, dry skin, gut issues. Yet all blood tests "normal".
2 weeks into my changes, it began to lift. I have a long way to go but all conventional medicine could offer me was nasal steroids!
I try not be too evangelical to others with similar health issues as I know it's not everyone's cup of tea. But I do wonder why there isn't more focus by governments into diet and modern living and the rise in all the weird issues mentioned by rawcoconut...
For clarity! Grains=evil .
Magnesium- it very poorly absorbed from suplements and even from food, which is why the body using magnesium stores to process grain toxins is a problem- it leads to deficiencies.
Magnesium baths are good (dead sea salts for example), it's expensive but you can cut it with Epsom salts, as the sulphates in that are good too. Both are good for the skin but may initially cause minor rash (the magnesium salts anyway) so use smaller amounts to start with.... Or you could swim in the sea daily!
Ohdsddypig - yes, there are good reasons why traditional cultures prepared food stuffs in certain ways.
I don't think I would try the sprouted or soaked, fermented grains now, but I bet if I'd been raised eating them, I would never have developed the food allergies, intolerances and immune issues.
I think modern preparation methods as well as the sheer amount of grain foods we are told to eat are both a big part of the problem.
Ohdaddypig- cholesterol check, remember its not the total number that's the important thing but the ratio of high to low density cholesterol, so a higher number, but with a healthy ratio is better than a low number with the wrong ratio,
And testing you numbers now, is not going to tell you what it was before paleo- just suppose you get a bad result, maybe it was worse before paleo iykwim, so retest in 3 or 6months...
Hello, everyone! Haven't been on any paleo/primal threads before but I saw this one and thought it would be nice to join in
I'm a relative newcomer to paleo - I first started reading/thinking about it when I read Escape the Diet Trap last year but was a veggie (of 20 years' standing!) at the time so it didn't really appeal. Then at the start of this year I joined a CrossFit gym and started rethinking what I was eating.
I've now been doing paleo for about 5 months and have started eating fish and meat again (experimentally). I have lost nearly a stone since the start of the year and feel much better when I'm eating this way, although I still get guilt pangs sometimes! Unfortunately I haven't been able to keep up the CrossFit due to money/location issues but I have signed up for Tough Mudder in September and am running and doing kettlebells at home in preparation for that. I never used to have much of a sweet toothy but I find that now it's my biggest weakness - I give myself Saturdays off so I can indulge.
Hi, thread dropped off my active list, summer holidays really interfering with my MN time
The summer holidays are also really interfering with my paleo diet! Doing the best I can - still off bread at least (it just seems so unappetising now!) - but just can't seem to settle into a routine of meals, keep missing at least one which means I usually end up eating SAD food.
Hi all Wildings what exactly is cross fit. I would love to try kettlebells but cannot find an instructor. One of the paleo books I can get from the e library goes on about kettlebells being good for womens sex drive - fraid thats not the reason I want to try them though - apparently its an efficient way to excersise.
hello, I'd like to join. I want to cut gluten and sugar, so paleo seems a good approach. But I live on sugary carbs so it's going to be a tough one!
Please could you give me some ideas of quick, non-offally meals for the week ahead?
Hi Giraffes!
CrossFit is basically a sort of combination of weightlifting & gymnastics moves that you do in a group. It's supposed to turn you into the perfect all-rounder - good for strength, flexibility, stamina etc. It's very high-intensity and quite scary to start off with but I really loved it! Just sad that I can't do it now due to money/travel issues. There are a couple of videos here and here (disclaimer - I am nowhere near that level of fitness!)
Kettle bells are a great exercise tool - swings, especially, are a really good & quick way to build up core strength - but technique is really important and you really need someone to check that you're doing them properly as you can put your back out otherwise.
I'll hold your hand snow
As someone with an acidic mouth, sugar is pure evil. It burns like hell! Have you tried switching to a Lauryl sulphate free toothpaste?
After a couple months without Lauryl Sulphate, I was able to eat sweeter fruit. I still don't go anywhere near sugar, molasses but I might taste an ice-cream with honey.
Hello BeingMoreDog and welcome.
Ideas for the week ahead:
Butternut squash mash take only 5 minutes to cook.
Steamed vegetables with butter or olive oil (and lemon).
Stir fries.
Sweet potato, swede, butternut squash chips in the oven take about 15 minutes
Fish can be ready in 5-10 minutes
Minute steaks are incredibly versatile ( saltimbocca , pizzaiola and other sauces )
Avocado caprese with rocket salad
Avocado (mashed with garlic, lemon, chopped basil and a pinch of seasoning) with prawns or tuna served on lettuce leaves.
They sound delicious fava - I love avocado. Looking fwd to shopping tonight so can get going on this!
How about snacks? I need to reduce the sugary snacks, but keep the fatty ones as breastfeeding. I don't want to lose weight (could do with gaining a bit tbh), but I need to eat a lot more healthily.
Is natural yoghurt a go-er? I love that with seeds and nuts.
I love full fat Greek yog with seeds/ nuts berries too (also BF)
One month since starting this
Ten pounds down
Body shape changed.
Back in size 12.
Bikini pic on profile eeekk!
Well done trucks that's a great result.
Interesting Fava, will definitely give the sls free toothpaste a go - can you recommend one that doesn't taste hideous? I haven't had a flare up since I've gave up sugar and fruit, my mouth is still sore but bearable and doesn't interfere with eating and drinking. Off to google acidic mouth....
Am on my way back from a week being fed by in-laws, and I feel grim. Am writing a grocery shopping list on the train and if I get home in time to do grocery shopping will do a reset Whole30 starting tomorrow.
Ultradex toothpaste and mouthwash (colour, flavour and alcohol free & PH neutral) are very good.
Well done, trucks
Yami, I will join you with whole 30 tomorrow (well at least whole 14, dp on hol end of month) I need boundaries!
Thanks wilding will check it out
BeingMoreDog - welcome!
Fav those ideas sound yum. Do you defrost frozen fish or just put it straight in oven?
OMG just watched the second cross fit link - safe to say no way i am doing that don't think I could even get onto a rope never mind climb up it....and as for flickflacks and hand stand push ups ! I was not expecting that - 17 year old girl is like spiderman!
Brilliant Fava just got some. What is acidic mouth, have googled but it's a term that seems to be a applied to lots of conditions.
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So I just recently had issues with our ATM not giving money to customers although the receipt prints out saying it did. This is obviously a HUGE issue. I called the company to complain and then asked what to do about the customer's money and he gets all pissy at me and tells me how federal law doesn't require him to reimburse people and they need to file a claim with their bank.
This was all news to me. I guess I had never thought about what happens when an ATM screws up. What if you had $105 in your account and you withdraw $100 but the ATM doesn't give it to you? You've not no access to money and no way to prove that you didn't get it right?
It's fucked up to say the least. I'm working with my corporate office to get this fixed for my customers, but sheesh.

subset: commoncrawl


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Russian firm Egorov Puginsky Afanasiev & Partners is boosting its capital markets practice with the hire of a team led by the managing partner of corporate and finance boutique Liniya Prava.
Dmitriy Glazounov and a team of nine lawyers and two support staff start work at Egorov Puginsky today (14 March). Glazounov will head up the larger firm's banking and finance and capital markets practices in Moscow.
Glazounov has significant experience in capital markets and before founding Liniya Prava held senior roles at the Federal Commission for the Securities Market of Russia and the Agency for Housing Mortgage Lending.
He advises on debt and equity capital markets and securitisations. His clients include major international and Russian financial institutions such as BNP Paribas, Morgan Stanley, Citibank, VTB Capital, Renaissance Capital and VEB Capital as well as corporates.
Glazounov said the combination of Egorov Puginsky's size and breadth and his team's experience could create the strongest banking and finance and capital markets practice in Russia.
Egorov Puginsky, which recently won European Law Firm of the Year at The Lawyer's European Awards (21 February 2014) has made only a handful of lateral partner hires in the last few years. In late 2012 it picked up legacy Herbert Smith partner Robin Wittering to build its English law capacity (21 November 2012), while three years ago it hired Baker & McKenzie's St Petersburg litigation head Ivan Smirnov (21 April 2011).
For more on capital markets work in Russia, see last year's special report, Market force.


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Nancy Spain practices in the area of general and probate related law. Prior to moving to Chicago in 1981, Ms. Spain was engaged in private law practice in Cleveland, Ohio. More »
Richard Spain concentrates his practice in estate planning, probate work, real estate, and corporate representation. A practicing attorney in Cleveland, Ohio for seven years, Mr. Spain returned to his hometown of Chicago in 1982. More »
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Deborah B. More »
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Mike's passion for helping people led him to open Oak Wealth Advisors in 2008 so that families with special needs members seeking experienced and objective financial advice could access the same quality of service that has traditionally been available only to the very wealthy. Having a brother on the Autism spectrum, Mike has a first-hand understanding of the challenges and joys living with a family member with special needs bring. His mission is to assist individuals and families in achieving financial security and enjoying their lives to the fullest extent possible. Mike's career includes leadership positions with three of the most respected independent financial advisory firms in Chicago prior to his founding Oak Wealth Advisors. In addition to his professional experience, Mike has served as president and/or treasurer of a number of Chicago-area special needs charitable organizations. Mike enjoys sharing his expertise and has done so through teaching classes on special needs financial planning at Texas Tech University, the University of Illinois, and the University of Wisconsin. He has also written articles for the Chicago Sun-Times, and has been featured or quoted in numerous national publications including The New York Times, The Wall Street Journal, Money Magazine, and Financial Planning. More »
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'The traffic is heavy today because it's a Sunday, everyone is out 'gezing' (sight seeing etc..).'
'The traffic is heavy today because it's a Monday morning and everyone is going to work.'
'The traffic is heavy today because it's Saturday afternoon' - no reason usually given.
'Of course the traffic is heavy today because it's a weekday.'
Add all the above up and you will understand traffic is always heavy here.
However, if you add snow to the equation, it all gets a lot more exciting! When I say snow, really we're talking about a fairly light brushing of soggy snow that comes most years for a day or so in January or February in Istanbul. But when it comes it's great! The children eagerly scan the local news in the hope that the Mayor will call a 'Kar Tatili' or Snow Holiday. The teachers take a passionate interest in the weather which is a restrained adult version of the kid's blind excitement about the possibility of a day off. Despite the excitement, in true Istanbul style the sun can rise and bring crashing disappointment to millions of hopeful hearts.
Never mind, the winter is yet young.
A piece of the city wall - nothing to do with snow, it's sunlight...……
Watercolour on 300gm paper.


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Updated Jan. 1, 2009 -- Times may be tough, but leaving our communities behind can't be an option. Through charity and volunteerism, come benefits – and not just for those on the receiving end of our generosity. Community service is a form of encouragement to those who have lost their way, to those who are developing in our world and to those who need that extra push.
With giving comes a sense of responsibility and purpose. People who are in need feel respected, feel necessary and may want to follow the path of giving back to their respective areas.
Through our help, we are able to build bridges. We are able to help strengthen the weakest parts of our community, which means that we are helping build a stronger world for ourselves.
Community service also allows us to shatter barriers. Just as those before us overcame obstacles and economic hardship, it is our responsibility to keep moving our community forward. Every little bit helps. You can volunteer at the nearest shelter, at a church, and even with the 2008 elections. So, step up, volunteer, and serve your community.
Start today!


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| Bipedal Hunter Killer
The FK Reaper, or Reaper class assault machine, was a bipedal HK unit utilized by Skynet in the alternate future of Terminator 3: The Redemption.
An FK Reaper possessed four optical sensors and an arsenal comprising a heavy plasma gun and a flamethrower.
The FK Reaper is apparently compatible with T-850 CPUs, allowing John Connor to install the chip of his protector into one such unit.


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Dear White Moms
I was reading this article and it touched me. I wanted to share it with you ladies because no matter what color we are this is a very important topic. Let me know what you think about the article!
That whole thing makes me so angry I am shaking! I have very strong opinions about this whole thing that might shock people. And my husband who happens to be black is in total agreement. This whole "unarmed black kid" is so much made up racism it's not even funny! This "unarmed kid" attacked a police officer while the officer was still in his car. There are so many examples of REAL racism in the world that to make stuff up just so the parents and organizations can make money off of lawsuits, completely makes the real racism that happens in this country seem less legit. Instead of making the world a better place for my children it is making it a place where the only cases of racism that we tackle are ones that money can be made from even if we have to create the racism...grrrr I could so go on and on!
I will admit that I haven't really kept up with the story as much as I would have liked to. So he attacked an officer?
Him and his friend were walking down the middle of the street so the officer tried to stop them and before he could get out of the car he went after the officer, tried to get the officers gun, a shot went off and then him and his friend ran. The friend was trying to get into the passenger side if the car. The officer got out of his car, told them to stop and the friend hid behind a car while the guy that got shot started walking toward the officer, he was about 6-7 feet away when he was shot but he was refusing to stop. The officer shot him not knowing that he was unarmed because he never got the chance to check. This guy had already attacked him and was now refusing to stop. I would have shot too. The officer went to the hospital with a swollen face because of the attack of this "unarmed kid" if it were a case if racism then why didn't the other friend get shot? Plus this officer has worked for that same department for 6 years with no disciplinary actions.
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Research Presentation
Kogod Classroom T61
Presenter: Yujin Jeong, Assistant Professor of International BusinessTitle: "Status and Bribery: Evidence from the Revealed Accounting Records of Two South Korean Presidents" (abstract attached below)
This co-authored paper will be presented to the Annual World Bank Conference on Development Economics 2015 in June.
Dean's office will support lunch (pizza, sandwiches and drinks) for participants. …more
Type: Conferences. Panels. Speakers
Host: Kogod School of Business


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Reciprocal Repression Between MicroRNA-133 and Calcineurin Regulates Cardiac Hypertrophy
A Novel Mechanism for Progressive Cardiac Hypertrophy
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Cardiac hypertrophy involves a remodeling process of the heart in response to diverse pathological stimuli. Both calcineurin/nuclear factor of activated T cells pathway and microRNA-133 (miR-133) have been shown to play a critical role in cardiac hypertrophy. It has been recognized that the expression and activity of calcineurin increases and miR-133 expression decreases in the hypertrophic heart, and inhibition of calcineurin or increase of miR-133 expression protects against cardiac hypertrophy. Here we tested the interaction between miR-133 and calcineurin in cardiac hypertrophy. Cardiac hypertrophy in vivo and in vitro was induced by transverse aortic constriction and phenylephrine treatment. mRNA levels were measured by using real-time PCR methods. Luciferase assays showed that transfection of miR-133 in HEK293 cells downregulated calcineurin expression, which was reversed by cotransfection with the miR-133–specific 2′-O-methyl antisense inhibitory oligoribonucleotides. These results were confirmed in cultured primary cardiomyocytes. miR-133 expression was downregulated, and calcineurin activity was enhanced in both in vivo and in vitro cardiac hypertrophy models. Treatment of cells and animals with cyclosporin A, an inhibitor of calcineurin, prevented miR-133 downregulation. Moreover, the antisense oligodeoxynucleotides against the catalytic subunits of calcineurin Aβ and the decoy oligodeoxynucleotides targeting nuclear factor of activated T cells transcription factor, a calcineurin downstream effector, increased miR-133 expression in cultured primary cardiomyocytes. Our data show that reciprocal repression between miR-133 and calcineurin regulates cardiac hypertrophy.
MicroRNAs (miRNAs) are small noncoding RNA molecules that silence their cognate target genes by specifically cleaving messenger RNAs or inhibiting their translations.1 MicroRNAs have emerged as important regulators of cardiac hypertrophy, and several miRNAs involved in heart hypertrophy have been identified, that is, miR-133, miR-195, miR-1, miR-208, miR-21, and so forth.2–6 Carè et al2 reported that the expression of microRNA-133 (miR-133) decreased in mouse and human models of cardiac hypertrophy. Overexpression of miR-133 in vitro inhibited cardiac hypertrophy. In contrast, suppression of miR-133 by "decoy" sequences induced hypertrophy. Therefore, changes of miR-133 were considered as the requisites for determining cardiac hypertrophy. Calcineurin is a calcium/calmodulin-activated serine-threonine phosphatase that dephosphorylates the transcription factor, nuclear factor of activated T cells (NFAT), which translocates into the nucleus to bind to DNA and activate hypertrophic response genes. Cardiac-specific activation of calcineurin or its downstream effector NFAT is sufficient to induce cardiac hypertrophy,7,8 and calcineurin protein levels and phosphatase activities increase in hypertrophic hearts.9,10 In light of the antithetical functions of calcineurin and miR-133, we hypothesized that calcineurin is the target of miR-133, and the expression of miR-133 is regulated by the calcineurin/NFAT signaling pathway.
Construction of Luciferase-miRNA Target-Site Fusion Plasmids
To construct reporter vectors bearing miRNA target sites, we synthesized fragments (by Invitrogen) containing the target sites for miR-133 and inserted these fragments into the multiple cloning sites downstream of the luciferase gene (HindIII and SacI sites) in the pMIR-REPORT luciferase miRNA expression reporter vector (Ambion, Inc), as reported previously.11
Synthesis of miRNAs and Anti-miRNA Antisense Inhibitors
Detailed methods are described in the online Data Supplement (please see http://hyper.ahajournals.org).
Luciferase Activity Assay
Detailed methods are described in the online Data Supplement.
Real-Time PCR Analysis
Unless otherwise stated, real-time PCR experiments were repeated 5 times. Detailed methods are described in the online Data Supplement.
Western Blot for Calcineurin and Calcineurin Phosphatase Assay
Detailed methods are described in the online Data Supplement.
Pressure-Overload Cardiac Hypertrophy
Detailed methods are described in the online Data Supplement.
Preparation of Primary Rat Cardiomyocytes
Cardiomyocyte cultures were prepared by dissociation of 1-day–old neonatal rat (Wistar) hearts and were differentially plated to remove fibroblasts.7 To induce the hypertrophic response, phenylephrine was added to cardiomyocyte cultures at 50 μmol/L, the concentration which has been proven to induce stable cardiomyocyte hypertrophy in our preliminary tests and another previous study.12,13 The culture medium containing phenylephrine was changed every 12 hours for a period of 72 hours. Cyclosporin A (CsA) was present at 0.5 μg/mL to inhibit cardiomyocyte hypertrophy.7 Cardiomyocytes were prepared for immunocytochemistry, as described previously.7 Monoclonal antibody against sarcomeric α-actinin (Sigma) was added at dilutions of 1:200. Nuclear staining was performed with 1.3 μmol/L of bisbenzimide (Sigma). The relative surface area of the cell was calculated from the number of pixels by using Image-Pro Plus (version 5.0.1).
Synthesis of NFAT Decoy Oligodeoxynucleotide and Calcineurin Aβ Antisense Oligodeoxynucleotide
NFAT decoy oligodeoxynucleotides (ODNs) are 5′-CGCCCAAAGAGGAAAATTTGTTTC-3′ and 3′-GAAACAAATTTTCCTCTTTGGGCG-5′. Scrambled decoy ODNs are 5′-TAGTTATGCATCACGACCTGAT-3′ and 3′-ATCAGGTCGTGATGCATAACTA-5′. Phosphorothioate-modified ODNs (20 bases in length) were used as antisense ODNs against the catalytic subunits of calcineurin Aβ and scrambled ODNs. Sequence (5′ to 3′) of antisense-Aβ is GCCATGCTGGGCCCGGGGCT; sequence (5′ to 3′) of scrambled-Aβ is GCGCAGTGCGGCGGTCCGTC. These antisense ODNs have been described and evidenced previously.14,15 All of the ODNs were synthesized by Shanghai Sangon Biological Engineering and Technology and Service Co, Ltd.
Transfections of microRNA, 2′-O-methyl antisense oligonucleotides (AMO), the decoy ODN, or antisense ODN were performed by using Lipofectamine 2000 (Invitrogen, Life Technologies), according to the procedure specification.
Data Analysis
Data are presented as mean±SEM. Significance was determined by using the Student t test for comparison of 2 groups and 1-way ANOVA for comparison of 2 more groups in SigmaStat Analysis Software. For t test, if normality failed, Mann–Whitney rank-sum test was used. P<0.05 was considered significant.
miR-133 Targets Calcineurin
We compared miR-133 nucleotide sequences with the sequences in the 3′-untranslated region (3′UTR) of calcineurin mRNA in rats and mice and found that the 3′UTR of calcineurin mRNA in the 2 types of species contains sequences that were imprecisely complementary to miR-133. There are ≥6 nucleotides matching the 2 to 10 nucleotides from the 5′ end of miR-133 in mice and ≥6 matching nucleotides in 1 site (site 1) in rats (Figure 1A). Because there are 2 possible binding sites in the 3′UTR of calcineurin mRNA in rats, we synthesized the fragments containing both site 1 and site 2, respectively. We performed luciferase reporter assays in HEK293 cells that do not express miR-133 (miRNA Research Guide, http://www.ambion.com/techlib/guides/Ambion_miRNA_res.pdf) to test whether miR-133 targets calcineurin in rats and mice. Compared with the negative control (NC) using chimeric constructs, transfection of miR-133 with the luciferase reporter gene linked to the wild-type 3′-UTR of calcineurin resulted in a significant decrease (P<0.01) of luciferase activity, and coapplication of miR-133 with its inhibitor, miR-133-specific 2′-O-methyl antisense inhibitory oligoribonucleotide (AMO-133), alleviated (P<0.05) the decrease of luciferase activity, whereas AMO-133 alone or mutant miR-133 had no effect (Figure 1B). These data suggest that calcineurin is the target of miR-133. To identify the importance of site 1 and site 2 in the interaction between miR-133 and calcineurin in rats, we further applied the luciferase reporter gene linked to the wild-type 3′-UTR of calcineurin with site 1 and site 2, respectively. Results showed that the effect of site 1 was equivalent to that of both sites, but site 2 had less effect than both sites and site 1 alone (see Figure S2 in the online Data Supplement), indicating that site 1 was predominant in the 2 sites.
Next, we tested the regulation of calcineurin by miR-133 in cultured primary neonatal rat cardiomyocytes. We examined the reliability of transfection with lipofectamine 2000 reagent. miR-133 level in cultured primary cardiomyocytes transfected with miR-133 increased ≈10-fold compared with the nontransfection group (Figure 2A, left). Mir-1, another skeletal specific microRNA, was the control and showed no difference between miR-133 transfection and nontransfection groups (P>0.05; Figure 2A, left). Because the cultured primary cardiomyocytes express endogenous miR-133, we checked the effects of AMO-133 and NC on the miR-133 level. Results showed that the NC had no effect on the miR-133 level, but AMO-133 decreased the miR-133 level significantly (Figure 2A, right). These data indicated that the experimental conditions were reliable.
In the cultured primary cardiomyocytes without phenylephrine treatment, transfection with miR-133 or cotransfection with miR-133 and AMO-133 did not affect the protein and mRNA level of calcineurin, but transfection of AMO-133 alone significantly increased the protein (Figure 2B) and mRNA level (Figure 2C) of calcineurin and induced cardiomyocyte hypertrophy (Figure 2D; P<0.01). In cultured primary cardiomyocytes treated with phenylephrine (50 μmol/L), transfection of miR-133 significantly decreased the elevated protein and mRNA level of calcineurin induced by phenylephrine (P<0.01), and coapplication of AMO-133 with miR-133 antagonized the effects of miR-133 (P<0.05; Figure 2E and 2⇑F). The changes of cell area were consistent with those of calcineurin protein and mRNA levels (Figure 2G). In addition, we also examined the protein level of calcineurin-B1 (CnB) in cultured primary cardiomyocytes to identify the specificity of miR-133 for calcineurin. In general, the protein phosphatase holoenzyme is composed of a catalytic subunit and regulatory subunit. CnB is a regulatory subunit. There were no statistical differences in the CnB protein levels among the NC, miR-133, miR-133+AMO-133, and AMO-133 groups (Figure S3A). However, compared with NC, phenylephrine treatment significantly increased the CnB protein level, and the increased CnB protein level was not affected by transfection of miR-133, miR-133+AMO-133, or AMO-133 (Figure S3B). These data indicate that miR-133 specifically targets calcineurin.
Calcineurin/NFAT Signaling Regulates miR-133 Expression
Conversely, it is not clear whether calcineurin regulates miR-133 expression. We established a cardiac hypertrophy model in vivo by transverse aortic constriction (TAC) in mice. The left heart ventricular tissues were used for examination of calcineurin protein level, calcineurin activity, and microRNA expression. As shown in Figure 3A and 3B, TAC induced cardiac hypertrophy, which was inhibited by treatment of the calcineurin inhibitor CsA. The protein level of calcineurin increased in the hypertrophic heart, and the increased calcineurin protein level was inhibited by CsA (Figure 3C). P-release represented the calcineurin activity, and the hypertrophic heart showed increased P-release, which was inhibited by CsA (Figure 3D). The expression of modulatory calcineurin interacting protein 1 (MCIP1, also known as regulator of calcineurin 1) was upregulated in response to calcineurin activation16; therefore, we further measured MCIP1 mRNA as a reflection of endogenous calcineurin activity. As shown in Figure 3E, MCIP1 mRNA expression was significantly increased in hypertrophic hearts, and the increased MCIP1 mRNA expression was inhibited by CsA treatment. These results were similar to the previous report.9 As shown in Figure 3F, the cardiac hypertrophy resulted in reduced miR-133 expression, as was reported by Carè et al.2 However, the decreased miR-133 expression was recovered by inhibiting calcineurin with CsA (Figure 3F). At the same time, we examined miR-195, which is another important miRNA involved in cardiac hypertrophy.3 As shown in Figure 3G, cardiac hypertrophy led to the increase in miR-195 expression, which was not affected by calcineurin inhibition, suggesting that there exists a specific correlation between calcineurin and miR-133. We also examined the protein level of CnB and fibrosis in sham and hypertrophic hearts treated with or without CsA. CnB protein level increased in TAC-induced hypertrophic hearts, and the increased CnB protein level was inhibited by CsA treatment (Figure S4). CsA also prevented cardiac fibrosis induced by pressure overload (Figure S5). Next, we confirmed the relationship between calcineurin and miR-133 in cultured primary cardiomyocytes that were treated with phenylephrine to induce hypertrophy in vitro. Phenylephrine induced cultured primary cardiomyocytes hypertrophy, which was inhibited by CsA treatment (Figure 4A and 4B). The protein level (Figure 4C) and activity (Figure 4D and 4⇓E) of calcineurin increased and miR-133 expression (Figure 4F) decreased in phenylephrine-treated cardiomyocytes. CsA inhibited cardiac hypertrophy (Figure 4A and 4⇓B), decreased calcineurin protein level (Figure 4C) and activity (Figure 4D and 4⇓E), and increased miR-133 expression (Figure 4F) in phenylephrine-treated cardiomyocytes.
Although CsA is a specific inhibitor of calcineurin, we further used antisense Aβ ODNs (antisense ODNs against the catalytic subunits of calcineurin Aβ) to test the effect of calcineurin on miR-133 expression in cultured primary cardiomyocytes. Results showed that antisense Aβ increased the expression of miR-133, and scrambled Aβ showed no effects (Figure 5A). NFAT is the immediate downstream effector of calcineurin. We inhibited NFAT by using NFAT-specific decoy ODNs (cis-element double-stranded oligodeoxynucleotides) to confirm that NFAT signaling regulated miR-133 expression. Results showed that NFAT decoy ODNs increased the expression of miR-133, and scrambled ODNs showed no effects (Figure 5B). These data suggest that calcineurin/NFAT signaling regulates miR-133 expression.
A clue to the mechanism of interaction between miR-133 and calcineurin in cardiac hypertrophy comes from the fact that the increased activity and expression of calcineurin were accompanied by the decreased miR-133 expression in the hypertrophic heart, which implies that there existed a specific relationship between calcineurin and miR-133. We found that miR-133 regulated calcineurin expression, whereas calcineurin/NFAT signaling regulated miR-133 expression. On the basis of our results, a model between miR-133 and calcineurin in cardiac hypertrophy was established. As shown in Figure 6, miR-133 or calcineurin regulates its own expression via a positive feedback, and the reciprocal repression between miR-133 and calcineurin regulates cardiac hypertrophy. Cardiac hypertrophy is a progressive process, and the mechanism remains obscure; the present study provides a novel understanding.
MicroRNAs are a class of endogenously expressed, noncoding tiny RNAs of 20 to 23 nucleotides, which are largely conserved in sequences and structures. miR-133 is divided into miR-133a and miR-133b, which are only different in 1 base in the terminal 3′ position. This differential base had no influence on complementary matching to mRNA. miR-133a is also divided into miR-133a1 and miR-133a2, for which the sequences are the same. miR-133a was used in the present study. Empirically, ≥6 nucleotides matching the 2 to 10 nucleotides from the 5′ end of miRNAs indicate that there would be existence of the target regulation by miRNAs.11 We identified target sites for miR-133 in calcineurin on the basis of complementarity, with ≥6 nucleotides matching the 2 to 10 nucleotides from 5′-end of miR-133 in mice and ≥6 matching nucleotides in 1 site (site 1) in rats (Figure 1A). Although there are 2 possible binding sites in the 3′UTR of calcineurin mRNA in rats, site 1 is predominant.
We found that transfection of AMO-133 alone significantly increased the protein and mRNA level of calcineurin and induced hypertrophy in the cultured primary cardiomyocytes without phenylephrine treatment, suggesting that endogenous miR-133 has physiological significance. On the other hand, in the cultured primary cardiomyocytes without phenylephrine treatment, transfection with miR-133 or cotransfection with miR-133 and AMO-133 did not affect the protein and mRNA level of calcineurin, suggesting that the amount of endogenous miR-133 is sufficient to inhibit calcineurin in the normal state; even if the miR-133 level is increased by exogenous transfection, calcineurin cannot be inhibited further.
Most animal miRNAs are imprecisely complementary to their mRNA targets, and they inhibit protein synthesis without interfering with the stability of the mRNA target. miR-133 is imprecisely complementary to calcineurin mRNA (Figure 1A); thus, calcineurin mRNA should not be cleaved and destructed directly. We found that the change of calcineurin mRNA was paralleled with that of calcineurin protein level, which might be attributed to the positive feedback mechanism of calcineurin itself. It has been reported that the calcineurin promoter is specifically activated through a positive feedback mechanism involving calcineurin-induced NFAT in the heart17; therefore, when calcineurin protein level decreases, the mRNA level will decrease correspondingly. For this reason, we speculate that the decrease of calcineurin mRNA level is not as a result of direct destruction induced by miR-133.
Although CsA is a specific inhibitor of calcineurin and has been used to specifically inhibit calcineurin in our and other previous works,7–9 we further used antisense Aβ ODNs and NFAT decoy ODNs to test the effect of the calcineurin/NFAT pathway on miR-133 expression. These 3 approaches, CsA, antisense-Aβ, and NFAT decoy ODNs, confirm that calcineurin/NFAT signaling regulates miR-133 expression. NFAT is a transcriptional factor, and the putative binding site for NFAT is 5′-A/TGGAAANA/T/C-3′.18 We found that the genes of miR-133 in rats and mice are Mib1 and EG622283 (from the genome database at the National Center for Biotechnology Information), respectively; however, the putative binding sites for NFAT could not be found from 700 bp upstream to 300 bp downstream of the transcription start site of these genes (the genome database at the National Center for Biotechnology Information). On the basis of the present knowledge, the ideal explanation for the regulation of transcriptional factors on miRNA expression should be the existence of binding sites for transcriptional factors, but the regulation of miRNA expression is so complicated that multiple factors may be involved in the procession from gene expression to miRNA maturity. Mature miRNAs are initially transcribed as primary miRNAs that require the RNase III enzyme Drosha in the nucleus to be trimmed into precursor miRNAs. The latter are exported by the nuclear export factor exportin 5 to the cytoplasm, where they are cropped to become mature miRNAs by another RNase III enzyme, Dicer.19 From this, it can be seen that it is unreliable to explain the regulation of transcriptional factors on miRNA expression simply by matching the binding sites. The possibility is not excluded that there exits an indirect regulation of miR-133 by calcineurin/NFAT pathway. Presently, the regulation of miRNAs is not understood in depth. We could not elucidate the exact molecular mechanism of NFAT regulating miR-133, which is the limitation of the present study.
Calcineurin/NFAT pathway or miR-133 plays a critical role in cardiac hypertrophy. Here we report that the reciprocal repression between miR-133 and calcineurin regulates cardiac hypertrophy. miR-133 regulates the expression of calcineurin through posttranscriptional repression. Conversely, calcineurin/NFAT signaling also regulates miR-133 expression. Our results indicate that, once calcineurin/NFAT signaling is activated, miR-133 expression will decrease with a loss of repression on calcineurin, and the heart will be progressively hypertrophic. We provide a novel understanding of progressive cardiac hypertrophy.
We gratefully acknowledge Xiao-Bin Luo (Research Center, Montreal Heart Institute, Montreal, Quebec, Canada) for providing sequences of NFAT Decoy ODNs.
Sources of Funding
This work was supported by National Natural Science Foundation of China (30873064), A Foundation for the Author of National Excellent Doctoral Dissertation of PR China (2007B72), and Program for New Century Excellent Talents in University of China.
- Received July 19, 2009.
- Revision received August 7, 2009.
- Accepted January 22, 2010.
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Carè A, Catalucci D, Felicetti F, Bonci D, Addario A, Gallo P, Bang ML, Segnalini P, Gu Y, Dalton ND, Elia L, Latronico MV, Høydal M, Autore C, Russo MA, Dorn GW II, Ellingsen O, Ruiz-Lozano P, Peterson KL, Croce CM, Peschle C, Condorelli G. MicroRNA-133 controls cardiac hypertrophy. Nat Med. 2007; 13: 613–618.
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van Rooij E, Sutherland LB, Liu N, Williams AH, McAnally J, Gerard RD, Richardson JA, Olson EN. A signature pattern of stress-responsive microRNAs that can evoke cardiac hypertrophy and heart failure. Proc Natl Acad Sci U S A. 2006; 103: 18255–18260.
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Sayed D, Hong C, Chen IY, Lypowy J, Abdellatif M. MicroRNAs play an essential role in the development of cardiac hypertrophy. Circ Res. 2007; 100: 416–424.
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van Rooij E, Sutherland LB, Qi X, Richardson JA, Hill J, Olson EN. Control of stress-dependent cardiac growth and gene expression by a microRNA. Science. 2007; 316: 575–579.
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Thum T, Gross C, Fiedler J, Fischer T, Kissler S, Bussen M, Galuppo P, Just S, Rottbauer W, Frantz S, Castoldi M, Soutschek J, Koteliansky V, Rosenwald A, Basson MA, Licht JD, Pena JT, Rouhanifard SH, Muckenthaler MU, Tuschl T, Martin GR, Bauersachs J, Engelhardt S. MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts. Nature. 2008; 456: 980–984.
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Dong D, Duan Y, Guo J, Roach DE, Swirp SL, Wang L, Lees-Miller JP, Sheldon RS, Molkentin JD, Duff HJ. Overexpression of calcineurin in mouse causes sudden cardiac death associated with decreased density of K+ channels. Cardiovasc Res. 2003; 57: 320–332.
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Lim HW, De Windt LJ, Steinberg L, Taigen T, Witt SA, Kimball TR, Molkentin JD. Calcineurin expression, activation, and function in cardiac pressure-overload hypertrophy. Circulation. 2000; 101: 2431–2437.
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Ritter O, Hack S, Schuh K, Röthlein N, Perrot A, Osterziel KJ, Schulte HD, Neyses L. Calcineurin in human heart hypertrophy. Circulation. 2002; 105: 2265–2269.
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Xiao J, Luo X, Lin H, Zhang Y, Lu Y, Wang N, Zhang Y, Yang B, Wang Z. MicroRNA miR-133 represses HERG K+ channel expression contributing to QT prolongation in diabetic hearts. J Biol Chem. 2007; 282: 12363–12367.
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Taigen T, De Windt LJ, Lim HW, Molkentin JD. Targeted inhibition of calcineurin prevents agonist-induced cardiomyocyte hypertrophy. Proc Natl Acad Sci U S A. 2000; 97: 1196–1201.
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Oka T, Dai YS, Molkentin JD. Regulation of calcineurin through transcriptional induction of the calcineurin A βpromoter in vitro and in vivo. Mol Cell Biol. 2005; 25: 6649–6659.
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Yang B, Lu Y, Wang Z. Control of cardiac excitability by microRNAs. Cardiovasc Res. 2008; 79: 571–580.
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- Reciprocal Repression Between MicroRNA-133 and Calcineurin Regulates Cardiac HypertrophyDe-Li Dong, Chang Chen, Rong Huo, Ning Wang, Zhe Li, Yu-Jie Tu, Jun-Tao Hu, Xia Chu, Wei Huang and Bao-Feng YangHypertension. 2010;55:946-952, originally published March 17, 2010https://doi.org/10.1161/HYPERTENSIONAHA.109.139519
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- Reciprocal Repression Between MicroRNA-133 and Calcineurin Regulates Cardiac HypertrophyDe-Li Dong, Chang Chen, Rong Huo, Ning Wang, Zhe Li, Yu-Jie Tu, Jun-Tao Hu, Xia Chu, Wei Huang and Bao-Feng YangHypertension. 2010;55:946-952, originally published March 17, 2010https://doi.org/10.1161/HYPERTENSIONAHA.109.139519Permalink:


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Set for Spring – For the spring 2012 season, German based label Set enlists the top model duo of Kasia Struss and Bambi Northwood-Blyth for its latest lookbook. The new range is inspired by the rock and roll aesthetic of the 1970's with casual denim, suede, leather and lightweight knits in a neutral color palette. Accessories include oversize hats, understated jewelry and bohemian accents for all the spring essentials.
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Suryadatta College of Management Information Research & Technology_duplicate is situated at Sr. No. 342, Chandani Chowk Campus, Patil Nagar, Near DSK Ranwara Society Bavdhan (Bk.), Pune - 411021, Maharashtra, India. in Pune. The Institute campus is about 8 km. away from Railway Station Shivaji Nagar, 8 km. away from Lal Maheal Bus Stop, 17 km. away from Pune Airport.
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Meguiar's new products for 2017 announced 10/31/16
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Rep. Ron Kind (D-WI) today voted to save and create nearly 320,000 jobs -- including saving the jobs of 160,000 teachers and preventing the layoffs of tens of thousands of police officers, firefighters, and nurses. The Education Jobs and Medicaid Assistance Act is not only completely paid for, it reduces the deficit by $1.4 billion over 10 years--through spending cuts and by closing a loophole that encourages corporations to ship American jobs overseas.
"Many communities in Wisconsin are being forced to choose between important public services," said Rep. Kind. "This bill changes that. "Our kids shouldn't have to wait for a full economic recovery to receive a quality education."
The legislation provides $10 billion in funding to save more than 160,000 teacher jobs nationwide. The bill is expected to fund approximately 3,000 K-12 jobs in the state and 284 K-12 jobs in western Wisconsin alone, according to the National Education Association. It also provides $16.1 billion in health assistance to the states that, by reducing shortfalls and stimulating the economy, will save and create 158,000 jobs, including preventing the layoffs of police officers and firefighters. More than half these jobs will be in the private sector, including workers who contract for or supply services to state and local governments.
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The funding in the bill is supported by a majority of the nation's Governors, including many Republican Governors, and by numerous organizations, including the bipartisan National Conference of State Legislatures, National Association of Counties and the Leadership Council of Aging Organizations.
Source: http://www.kind.house.gov/index.cfm?sectionid=52&parentid=1§iontree=52&itemid=716


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Liberal Bishop of Des Moines Promotes "Medical" Cannabis
Posted by Tancred
Edit: it's interesting to note how some prelates like Msgr. Pates, camouflage themselves by perfunctory attention to social issues from a Catholic point of view, and spend the rest of their time pushing an overriding modernist agenda. For example, few people know that Archbishop John Ireland was such a bishop since many of his public acts were irreproachable and even laudatory, but he was an Americanist and even contemplated a schism in the late 19th century.
[Des Moines Register] Des Moines' Catholic bishop is joining with Iowa lawmakers who are pushing to make medical marijuana legal in the state.
Bishop Richard Pates, the influential leader of 100,000 Roman Catholics in southwest Iowa, has asked House and Senate members to approve a comprehensive bill to permit medical cannabis. The Iowa Legislature is in an intense debate over medical marijuana legislation as the final days of the 2016 session wind down.
"I believe now is the time to help suffering Iowans and their families get access to this medicine," Pates said in a letter dated late last week.


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Yes, you would be able to configure your email account in Outlook or Outlook Express.
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Yes, you will be able to use your email account through IMAP or POP3 on your smart phone, and use the email account on your phone. The advantage of this would be, that you would not be restricted to the free email providers. You will be able to send email from your email @ yourdomain.com to anyone, right from your smart phone.
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Yes, you need to have your own domain name, for google to take you seriously. Targeted traffic from google will not come to you if you use a free email service, such as hotmail. Google gives a lot of importance to genuine content, and it would be extremely difficult for google to know that the content is yours if you use a free email address.
6. What other advantages are there to having a domain name and email address ?
It gives you privacy. You are away from the prying eyes of google and other free email providers. If you use a free email address, you are exposing your email data to the prying eyes of the providers. The providers would crawl the data and use that to send you contextual advertisements on the screen while you are checking your email. This is how they make money to provide you the service. You are also opening yourself up to others subpoenaing your email provider to furnish your email data.
It is always better to use your own private email address with your own hosting provider. This is not as expensive as it sounds, and most providers provide this service at about $ 2 per month for a small business, and at about $ 4 per month for larger organizations.


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|2006-04-13, 20:05
|Link #21
Junior Member
Join Date: Apr 2006
Location: NJ
Age: 30
It's all about Walden (if anyone ever had the guts to attempt reading it) if you don't agree with a law you must practice "peacefull disobediance." I say we march on washington that's who all the other opressed people got their unjust laws lifted.
Despite how well it works in anime, in the real world violence is not the anwser, it's just adding more fuel to the fire..
|2006-04-13, 20:09
|Link #23
Junior Member
Join Date: Apr 2006
Location: NJ
Age: 30
|2006-04-13, 20:15
|Link #25
Senior Member
Join Date: Nov 2003
Location: The dog gossips too much.
|2006-04-13, 20:16
|Link #26
Gangsta Member
its caus i had iss for 3 weeks, and i wasnt in class very often
i dont even bother to do work anymore
its a fucking waste of time
|2006-04-13, 20:20
|Link #27
Junior Member
Join Date: Apr 2006
Location: NJ
Age: 30
I might sound stuck up here, but if I were you I'd do my best to get an education and move out of you area and make a name for youself. There is always the GED approach.
|2006-04-13, 21:18
|Link #29
Senior Member
Join Date: Nov 2003
Location: The dog gossips too much.
In any case I'm not your counsellor or anything, so let's get back on topic. What was it again...?
|2006-04-13, 21:42
|Link #30
Monarch Programmer
Join Date: Apr 2004
Location: Liverpool
Age: 35
Ok a little off-topic here but I just have to tell you guys this.
In the city of Chester, England, it is legal to shoot a Welsh man with a Crossbow after Midnight. No lie. A crazy law from the old times that has not been changed to this day!
|2006-04-13, 22:47
|Link #31
Junior Member
Join Date: Apr 2006
Location: NJ
Age: 30
|2006-04-13, 22:48
|Link #32
Junior Member
Join Date: Apr 2006
Location: NJ
Age: 30
I think breaking the law is ok if you don't belive in it, as long as it fall into the societies moral guidelines.
So yeah back to the original question of this thread scenario 2 is worse, and I'd still be diriving 60.
|2006-04-13, 23:15
|Link #33
now with 20% more ego!
Laws were created to appeal to people the fact that they have to take responsibility for their actions. Usually, laws which are objected to would tend to range from ridiculous speed limits to more serious cases in which the manipulation of the judicial system is used in order to exploit laws for political or economic gain. With regards to the latter, one would have to argue if these laws are truly detrimental or not and if not, should they still be broken? In such cases, perhaps the breaking of the law is more justified, but on the other hand, though this is a representation of a failing of human morality, isn't breaking them because you object to them the same thing? Essentially, the answer to the main question posed in this thread all depends on the moral incentives of the person who's making the decision of if he should, or shouldn't, break a law because he or she disagrees with it. However I feel that yes, even if the law in question does not cause harm to people, they should be objected to because what they represent is an essential failing in morality with regards to the government which, in itself, is contradicting the very ideological concept of making up laws in the first place, that being putting in place a set of morally-ideal behavior for people to adhere to.
I don't think that without standardized law there'll be chaos. But likewise, that can only be the case if people and society as a whole became so morally-conscious that we would render laws obsolete, which of course will never happen because as history has proved on so many occasions, people have a tendency of being bastards.
Last edited by Inuzuka; 2006-04-14 at 06:32.
|2006-04-14, 01:01
|Link #34
I find it a little scary when I read posts from people with the attitude of newtyped but at 14 I guess we often say things we don't really mean in order to sound cool. Hopefully he or she will grow up and realise the importance of getting a good education and that, at the risk of sounding completely boring, education can be the key to a better future.
Anyway, back to the original post. Yesterday a doctor in the British army was sent to prison and fined BP20,000 for refusing to go to Iraq. His justification was that the war on Iraq was illegal. At first I thought maybe he was a bit nervous about going but then read that he had already served twice in Iraq so it couldn't have been that. He had moral principles that he wanted to uphold and look what happened to him.
With regard to the speed limit. Sure I do drive above the speed limit, just a bit. But then again the cause of many accidents is put down to speeding. So should we break the law with regard to speed? Probably not but if you drive at the speed limit every other driver gets really pissed off with you and then that can also cause an accident with other drivers trying to overtake you. So if everyone breaks the speed limit, does that make it right? I like to think that laws are there for the benefit of the majority even though I think some are stupid. For example should doctors be allowed to help you to kill yourself? At the moment they would be accused of murder but if the person trying to kill themselves is going to die anyway and just wants out before the pain or situation becomes intolerable shouldn't they be allowed to die with dignity?
I read a story about a woman in the late 19th Century who was brought up by a very moral/religious family. She was never told the "facts of life" and was married off to some old guy. She could not come to terms with the fact that she had to have sex with this guy so she threw herself out the window of the hotel. Unfortunately for her she didn't die. The doctor was given the job of trying to save her life so she could be taken to court and tried for attempted suicide so the authorities could then hang her. How sad.
|2006-04-14, 09:48
|Link #35
♪♫ Maya Iincho ♩♬
- Though violence isn't the answer, you read through history right? Peace was always won thru violence, then after the killing and bloodshed there was peace, even if it was for only a little bit.
- I agree with most of ya that. I believe that it's legal to break the law if you " Your entire self, 100% believe that you will no harm anyone."
scenario 1 - there's a 20 mile stretch of roads you're driving on 3am in the early early morning, and the speed limit is 60mph (miles per hour) (sorry i dont know the speed in Kilometers per hour, around 80-90KPH, somewhere around there). You are driving 90mph, the police pulls you over for speeding, you argue that it's 3am and there's no intersections for approx 5 miles and you still pull me over? Well, whether we like it or not, the speed limit is there 24/7/365.
This seems very logical if you breaking the law, but no one can be harmed in any way, except yourself, should you be arrested or fined for something that may/may not harm any other but yourself?
The officer/judge (who ever happens to be alledging you of your crime) may say that they are protecting you from yourself or maybe you from any bystander that will be eventually in the way.
I also agree with this. Without regulations, there would be no such a word as peace. Though my thoughts are paradoxical, there's just too many rules and regualtions around to agree with and to obey.
|2006-04-14, 10:35
|Link #36
Sexy Tornado
Join Date: Dec 2005
Location: The European Bunion
Age: 38
The sad thing is that with today's law system and the amount of unscrupulous lawyers in the business (I'd say profession but personally I don't consider it as such any more because there's little professionalism left and it's mostly about the money) it's entirely likely you could shoot a Welshman and kill him with using a crossbow, with no real consequences. There are that many stupid laws and of course stupid loopholes in the law that it's basically possible to do anything you want and get away with it, as long as you can pay for a bent enough lawyer.
|2006-04-14, 10:39
|Link #37
we girls arnt safe!
Join Date: Apr 2006
Location: In the space between your walls
Age: 29
Befor any of this we should all grab a book of laws and go to law school.........or not
But I think that some laws are made becase there are a baise for respect and ricousness, like no steeling, no killing, no abuse, etc.
But some laws are just bull to protcet those that fall in the reqirement or are the only ones who care.
so if a law dosent hurt the well being of a person over all (like O no I am a millionaire but that kid didn't buy my CD for 15$ he burnt it from his freind lest throw him in jail!) its not to bad to break.
mostly laws are there to protect the rich and bairly keep the normal people from geting steped ALLOVER.
all so if you break a law thats being abused or no used corectly or is againt worldly standers of well being, then grab your torch and pitch frok and lets go!
|2006-04-14, 15:17
|Link #38
Join Date: Mar 2006
Age: 28
it's pretty obvious that you don't know how to read, or the basics of grammar.
whatever schooling you're getting, it's not doing much good, truthfully.
and once you're 16, nothing is stopping you from dropping out.
to get back on topic, i really think it depends on the law. i used to smoke before it was legal for me to, etc.
|2006-04-14, 17:18
|Link #39
Deadpan Snarker
Join Date: Dec 2005
Location: The Neverlands
Age: 40
I'm a notorious speedlimit offender
I'm equal to a 'potential murderer' according to the leaders of the current witchhunt on speeders
I cannot see any justification in punishing speeding on an empty highway
if an area is known for accidents and they set up speedtraps there, ok! I'll accept that
problem is,.... speedtraps are nowhere to be found there, only where it's easy pickings
long open freeways with low traffic, where the streets are litterally paved with gold
with lame excuses like sound, enviroment and safety ...puh-lease
Oh, and you know how the get ya?
Obscured by bushes or other elements in the scenery like some goddamn group
of medival bandits.
...difference is they don't take your money at gunpoint and get away with it
they take a picture, get away with it, and 6 weeks later there's a bill on you doormat
"White collor Criminals" indeed
The "againsts"
"But you could get yourself killed!"
Uh-huh, I could also get stabbed by some kid with an attitude who I offended because
I accidently bumped into him
"You could end up paralyzed"
Ahhh, a real burden for society!
Now my taxmoney that had to go to alcoholics, junkies, psychos, lazy*** overpaid
bureaucrats and politicians not to mention those medival bandits with their fat
*** sitting in a car taking pictures like 2 dirty old men ....has to be spent on me me!
"You could kill someone else!"
I ride a motorcycle, no pedestrians on the highway, every car on the road is a
potential killer for me, not the other way around
and unless someone is going 30 on the left lane where they should be doing
100 on the right, chances of me rearending them with 270km/h are slim to none
Vehicles: Affectionatly "the holy cow of the west" and they're milking it for all it's worth
Signing off: Holland's most wanted
|2006-04-15, 08:27
|Link #40
♪♫ Maya Iincho ♩♬
The law can be more clearly understood if you understand the basis of it, what it's suppose to do and why it's there. If you can prove for a certainly fact that it's not necessary, then your statement should be valid.
|Thread Tools


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|In TX if you have a PPO they are required to cover LMs (Licensed midwives) . . .
This is a little OT, but I'm having trouble getting my insurance to pay for my last homebirth.
Peach - Where can I get that
in writing?
My insurance company is trying to say that they only cover CNMs, not CPMs . . .
In Texas, the insurance companies are supposed to cover homebirths but they often use a loophole. The loophole is that a lot of places are self insured but use the insurance company to "manage" the benefits. Because companies are self insured, they don't have to follow the "regular" guidelines.
Here is a link to a list of mandated health coverage in Texas:
Momma to DD (12/04) and DS (11/09) .
I survived 16 mos! Ask me about breastfeeding a baby with posterior tongue tie, high palate, and weak oral motor skills- whew!
Okay.... so if it really is using the insurance company (dh's company is I'm 90% sure...) then aetna's anti-homebirth policy is not legal, is that correct?
I've always heard it's not legal... but I'd like to catch them on it...
Technically, it isn't legal in Texas. Aetna will give you the run around on the phone and give you a bunch of excuses.
If you continue to use your regular midwife, I know she can get Aetna to pay. :-) The baby was born in April of 07 and Aetna paid the following Dec./Jan.
I found this other link with a summary of some of the insurance changes: http://www.texasbar.com/Template.cfm...&ContentID=972 (Make sure that you are not on an HMO.)
|My insurance company is trying to say that they only cover CNMs, not CPMs . . .
It boils down to, if they don't have to follow the law, it's not technically illegal. That doesn't mean they won't pay eventually. You can also (sometimes) speak to the people in HR who write the policy for your dh's company. They can force the insurance company to pay. Whether they will or not is a different question.
My CPM does not do the billing. Her clients pay her and then we do our own insurance ppwk. She will help if/when she can, but she's too busy with her actual job to do much about what our insurance companies are saying to us.
Anyway, thanks for the input!
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text: human trafficking Human trafficking, modern-day slavery By Jennifer White & Mercedes Mejia • Feb 6, 2012 UM Law School's Human Trafficking Clinic. Human trafficking is a growing crime in Michigan, and the U.S. At its most basic level, even identifying victims of this crime can be difficult. In the first of our three part series we'll look at the challenge of providing assistance to victims. Michigan Radio's Jennifer White spoke with Elizabeth Campbell, Staff Attorney at the University of Michigan Law School's Human Trafficking Clinic. Campbell says there are mostly two categories of human trafficking. Trying to curb human trafficking By Christina Shockley & Mercedes Mejia • Feb 6, 2012 ThomasWanhoff / Flickr Each Monday, as part of our "Seeking Change" series, we're checking in with someone who's trying to improve life for people in Michigan. I spoke this morning with Andy Sopher about a particularly difficult subject: sex trafficking of kids. Sopher is with Wedgewood Christian Services in Grand Rapids and is behind a new project aimed at curbing trafficking of youngsters. State attorney general files charges under state's new human trafficking law By Rick Pluta • Jul 26, 2011 Covair Owner / Flickr The state attorney general's office has filed the first charges under the Michigan's updated law against human trafficking. A man is accused of forcing two teen-aged girls in Detroit to become prostitutes. The man is charged with two counts of inviting teen-aged girls to parties and then forcing them to work as prostitutes, collecting all of the money, beating them for not earning enough, and sexually assaulting them himself. The attorney general's new Human Trafficking Unit is trying to extradite him from California. A study done last year for the Michigan Women's Foundation found as many as 160 cases a month of girls being sold online or through escort services in Michigan. The study did not track how often teen-aged girls and boys are offered on the streets or in hotel rooms. But human trafficking is becoming more common across the country. The Michigan Women's Foundation says the new charges and penalties are useful – but the state should also have a "safe harbor" law that ensures people forced to become prostitutes are treated as victims and not as criminals. Pages« first ‹ previous 1 2