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Zoya released a code that allows you to buy 3 polishes and only pay shipping. These polishes are free of toluene, formaldehyde, DBP (phthalates) and camphor, and wear wonderfully!
Code: FB2011
Thanks for passing on the code! It worked, only cost was for shipping. Excellent deal, for a really nice quality product. :)
Awesome deal, thanks so much for sharing!
Thanks, I ordered some and passed it along.
Mama to DS (5) and DD (2)
I ordered mine yesterday after getting my daily redplum email....! Ordering was seamless, though the site was slow, I'm guessing it was bogged down by many other people like me!
Thanks for sharing- can't wait to enjoy my new colors!
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|Reviews for Grasping For Control
| MysticWolf1 chapter 1 . 9/30/2005
Wow! I like it! It is kind of a psychological thriller.
| KaeKae chapter 1 . 3/10/2004
Well, well, well, the story begins. Characterization for the most part was pretty good. Trying to imagine Wes screaming "Bloody bastards" is fairly amusing.
Make sure that each person's lines are on different lines. Couple times at the end Angel and Donavon's lines were in the same paragraph, which just gets confusing.
Story seems very dialog driven, which is not necessarily a bad thing, because characterization seems close. Could have lived without Fred calling Donavon "Donnie", and maybe a description of whatever the hell Angel is supposed to give back to Donavon would be nice. Will probably get that in later chapters I'm guessing.
Overall I enjoyed it though. My main recommendation would be more description. But I'll definitely be back for the next chapter.
| Kamitose chapter 1 . 3/8/2004
yeah, the stupid document was coming up perfect on my computer but i never noticed it looking like this. I fixed it and the corrected version should show up soon. Thanks for pointing that out to me.
| Imzadi chapter 1 . 3/8/2004
I'd love to read this but the formatting is bad. There needs to be more spaces between paragraphs and the backslashes need to go.

subset: commoncrawl


text:
YP - The Real Yellow PagesSM - helps you find the right local businesses to meet your specific needs. Search results are sorted by a combination of factors to give you a set of choices in response to your search criteria. These factors are similar to those you might use to determine which business to select from a local Yellow Pages directory, including proximity to where you are searching, expertise in the specific services or products you need, and comprehensive business information to help evaluate a business's suitability for you. "Preferred" listings, or those with featured website buttons, indicate YP advertisers who directly provide information about their businesses to help consumers make more informed buying decisions. YP advertisers receive higher placement in the default ordering of search results and may appear in sponsored listings on the top, side, or bottom of the search results page.
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Lori Corrigan, MA, LCPC
Lori Corrigan, MA, LCPC
|Professions: Counselor, Mental Health Counselor, Psychotherapist
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Are you as healthy and happy as you would like to be? As human beings, we all face ups and downs in life. Sometimes we can work through life's challenges on our own, and other times it's more difficult to get through the difficulties the gift of life can bring.
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Although you probably associate mosquitoes with warm climates, 32 species have been recorded in Britain. These include species that transmit diseases elsewhere in the world. Although you may not consider them to be garden insects, there is a high chance that some species are breeding in water-filled containers in you garden from May onwards. They will happily live in water butts and any other reasonable sized container that has standing water; they have even been known to lay eggs in water dishes put out for pets. Mosquitoes lay their eggs in still water and begin life as aquatic larvae and pupae breathing oxygen from the surface. Adults emerge from the pupal stage and most female mosquitoes need to feed on blood to produce eggs. Most British species will bite humans, but they usually feed on frogs, birds, mammals and reptiles. The male mosquito only feeds on nectar and honeydew.
Fact File
The biggest mosquito is Toxorhynchites, with a wingspan of up to 2.5cm. It doesn't bite and the larvae eat larvae of other mosquito species.
Larvae of the mosquito Coquillettidia richiardii breathe oxygen by penetrating the vascular system of submerged aquatic plants.
Adult mosquitoes of the human-biting form of Culex pipiens are spreading worldwide from Africa by stowing away on airplanes. They have been caught on planes landing at Heathrow. Larvae also travel across oceans on boats transporting old tyres filled with water.


text: 11/19/16 10/13/07 POPSUGAR Food Salads Name That Dish! October 13, 2007 by partysugar 0 Shares Chat with us on Facebook Messenger. Learn what's trending across POPSUGAR. Walnuts are a great ingredient to pair with cheese, greens, and fruit. They are also an important component in the classic salad pictured below. This salad is one that combines crunchy apples, walnuts and crisp celery with a creamy dressing. Do you know what it's called? Name That DishSaladsWalnutsNuts


text:
Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite
Infestation of ovine skin with the ectoparasitic mite
Infestation with
This study has provided novel insights into the signalling mechanisms leading to the development of a pro-inflammatory response in sheep scab, whilst providing crucial information regarding the nature of mite factors that may trigger this response. It has enabled the elucidation of the temporal patterns by which the immune system is regulated following exposure to
Sheep scab, caused by the mite
The life cycle of
A major feature of sheep scab is the rapid epidermal influx of eosinophils and neutrophils, followed by blister formation and a serous fluid exudate resulting in dermal oedema
Our group recently demonstrated the up-regulation of the pro-inflammatory factor interleukin-8 (
Results & Discussion
Total RNA extraction from skin biopsy samples
Total RNA extracted from skin biopsies was assessed for quality based on the RNA Integrity Number (RIN) and quantified using a spectrophotometer
Microarray data processing
To ensure quality and consistency of sample labelling and array hybridizations, control information was collated from all arrays and reviewed prior to data analysis. Quality control data for all arrays was found to be consistent with the manufacturer's (Agilent) recommendations. The performance of the array hybridizations was further assessed through scatter plots, comparing each array with every other generated, in Genespring GX 11.0. Scatter plots confirmed a linear distribution between arrays and showed a dynamic uninterrupted range of expression values from low to high signals. Box and whisker visualizations confirmed the data had comparable distributions and were of sufficient quality for further analysis. To remove invariant transcripts that could contribute to multiple testing errors in the subsequent statistical analysis, downstream data filtering of the array dataset (15,208 probes) was performed. Probes showing a "present" or "marginal" flag call in 100% of the samples in any one of the 5 time points were considered reliably expressed (13,517 probes). This list was further filtered on expression values, retaining probes with 100% of the samples in any one of the 5 time points showing expression values within the 20-100th percentile. These filtering steps resulted in a final list of 10,716 probes for the differential expression analysis.
Determination of differentially expressed transcripts
Infestation of the skin with
Figure 1
Profile plot of 1,552 genes significantly differentially expressed during time course of infestation with
Profile plot of 1,552 genes significantly differentially expressed during time course of infestation with
Quantitative real time PCR (qRT-PCR) validation of microarray data
To confirm the validity of the microarray results, qRT-PCR confirmation was undertaken for 11 putative differentially expressed genes (Table
Table 1
qRT-PCR validation of microarray data
Gene Symbol
Microarray fold change
qRT-PCR fold change
1 hr
3 hr
6 hr
24 hrs
1 hr
3 hr
6 hr
24 hrs
Key - Comparisons between baseline samples (time = 0) and individual time points. *Correlations performed using Pearson's correlation co-efficient for highly expressed genes, i.e.
Temporal modulation of the host inflammatory response
All significantly differentially expressed genes were grouped into distinct clusters based on the time point (post-infestation) at which their expression level peaked. This produced eight distinct gene clusters with defined temporal peaks of expression across the time course of infestation (Table
Table 2
Peak temporal gene expression clusters following infestation with
Cluster ID
Cluster Description
Number of genes in cluster (% of total)
Immediate early (IE) up
81 (5.2)
Early (E) up
201 (12.9)
Intermediate (IM) up
344 (22.2)
Late (L) up
383 (24.7)
Immediate early (IE) repressed
6 (0.4)
Early (E) repressed
35 (2.3)
Intermediate (IM) repressed
289 (18.6)
L (L) repressed
213 (13.7)
Figure 2
Clustered mean temporal gene expression profiles following infestation with
Clustered mean temporal gene expression profiles following infestation with
Additional file 1
Gene lists for individual clusters. The file contains the list of genes represented in each of the individual gene expression clusters described in the text. This file includes gene symbol annotation where available, Agilent probe ID, fold change data for each gene at each time point compared to baseline (Time = 0) and the Benjamini & Hochberg FDR corrected p-values for each individual probe.
Click here for file
Comparison of the host response to
To identify key pathways involved in the host skin response to infestation with
Network/Pathway analysis of temporal host response to
The differential regulation of individual genes does not necessarily relate to the importance of a biological effect as these changes can also be due to indirect or bystander effects. This can be a particular problem when these changes are analysed in isolation. To limit the impact of these effects we utilised a network/pathway approach, analysing global changes in gene expression in the context of known signalling pathways, enabling the identification of functional groupings of genes. To identify the temporal mechanisms and signalling pathways involved in the host response to
Table 3
Top 10 fold-changing genes from each temporal cluster following infestation with
Cluster ID
Gene Symbol
Gene Description
Fold Change*
Transmembrane protein 123
CD83 molecule
Activating transcription factor 3
Cysteine-rich, angiogenic inducer, 61
BTG family, member 2
early growth response 1
cysteine-rich, angiogenic inducer, 61
G protein-coupled receptor 171
growth arrest and DNA-damage-inducible, beta
Chemokine (C-C motif) ligand 2
Chemokine (C-X-C motif) ligand 2
Selectin P (granule membrane protein 140 kDa, antigen CD62)
17.2 to 18.1
Suppressor of cytokine signalling 3
colony stimulating factor 3 (granulocyte)
phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
tumor necrosis factor, alpha-induced protein 6
Pentraxin 3
225.6 to 317.2
Matrix metallopeptidase 1 (interstitial collagenase)
237.1 to 302.8
Chemokine (C-X-C motif) ligand 5
Serine dehydratase
Metallothionein 1E
membrane-spanning 4-domains, subfamily A, member 8B
superoxide dismutase 2, mitochondrial
291.7 to 617.4
Small proline-rich protein 2A
Casein beta
small proline-rich protein 2E
109.7 to 163.2
S100 calcium binding protein A9
asialoglycoprotein receptor 2
S100 calcium binding protein A12
Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
Zinc activated ligand-gated ion channel
Heat shock 70 kDa protein 8
ADAM metallopeptidase with thrombospondin type 1 motif, 2
Fibrinogen-like 2
Secreted frizzled-related protein 4
Thioredoxin interacting protein
-3.4 to -3.9
Serpin peptidase inhibitor, clade F, member 1
Frizzled homolog 2
-2.6 to -2.7
Complement component 3
Fc fragment of IgG binding protein
Serum deprivation response
nitric oxide synthase trafficker
Family with sequence similarity 13, member C
Cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Lix1 homolog (mouse)-like
Scavenger receptor class A, member 5 (putative)
collagen, type III, alpha 1
-4.9 to -5.1
Cytochrome P450, family 2, subfamily F, polypeptide 1
CAP, adenylate cyclase-associated protein, 2
Insulin-like growth factor binding protein 1
Stathmin-like 2
GDNF family receptor alpha 2
Cytochrome P450, family 2, subfamily B
Keratin 1
Flavin containing monooxygenase 3
Key - *Fold change calculated relative to baseline samples (Time = 0), range given where 2 or more probes for a gene are present. #Cluster 5 contained only 6 genes
Table 4
Top five canonical pathways associated with each gene cluster following infestation with
Temporal cluster*
Canonical Pathway
IL12 signalling and production in macrophages
IL10 signalling
Aryl hydrocarbon receptor signalling
PPAR signalling
Production of nitric oxide and reactive oxygen species in macrophages
Dendritic cell maturation
TREM1 signalling
NF-kB signalling
IL10 signalling
NF-kB signalling
Acute phase response signalling
TREM1 signalling
Leukocyte extravasation signalling
Antigen presentation pathway
T helper cell differentiation
CD28 signalling in T helper cells
Role of pattern recognition receptors in recognition of bacteria and viruses
Acute phase response signalling
Role of macrophages, fibroblasts and endothelial cells in RA#
Wnt/beta-catenin signalling
Antiproliferative role of somatostatin receptor 2
CCR5 signalling in macrophages
Integrin signalling
IL1 signalling
BMP signalling pathway
TGF beta signalling
IL6 signalling
Wnt/beta-catenin signalling
Key - *Cluster 5 contained too few genes for effective analysis of canonical pathways. #Rheumatoid arthritis. Ap-value calculated using Fisher's exact test determining the probability that association between genes in the data set and canonical pathway is due to chance alone
Genes up-regulated with infestation, gene expression clusters 1-4
Cluster 1 - One hour post infestation
Cluster 1 represents the immediate early (IE) group of genes with peak expression at 1 hpi. The top biological function represented was "cancer" with 34 of the 51 pathway-eligible genes in this cluster associated with this category. The enrichment for cancer related genes is related to the abundance of transcription factors represented in this cluster and to highlight this, the top 2 networks from the Ingenuity pathway analysis were combined, forming a single network highly enriched for transcriptional regulators (Figure
Figure 3
IPA network depicting relationships among transcription factor encoding genes in cluster 1, 1 hour post-infestation with
IPA network depicting relationships among transcription factor encoding genes in cluster 1, 1 hour post-infestation with
The NF-kB family members
Due to the increase in IL12 production following stimulation of APCs with LPS, TLR4 signalling has been associated with a Th1-type response
TNFα is up-regulated in human epithelial cells following exposure to HDM allergens and has also been detected in early stage lesions in sheep scab
In summary cluster 1 highlights the transcriptional response in the instigation of the host pro-inflammatory reaction to
Cluster 2 - Three hours post infestation
Cluster 2 represents the early (E) group of genes showing a peak expression at 3 hpi. Pathway analysis identified the rapidly expanding host immune response with 62 of the 124 (50%) genes in this cluster associated with "inflammatory disease". The merging of the top 4 networks from the analysis of cluster 2 highlighted the up-regulation of NF-kB and pro-inflammatory genes (Figure
Figure 4
IPA network depicting relationships among pro-inflammatory genes in cluster 2, 3 hours post-infestation with
IPA network depicting relationships among pro-inflammatory genes in cluster 2, 3 hours post-infestation with
IL1-α and IL1-β are pro-inflammatory cytokines proteolytically processed to form potent mediators of inflammation
As well as peak expression for a number of genes involved in TLR and NF-kB signalling pathways, cluster 2 included genes involved in the instigation of the host inflammatory response. The expression levels of selectin-E (
Mite-derived proteases, including serine, cysteine and aspartic proteases trigger host inflammatory responses
Sheep scab is classically associated with a Th2 type response, supported by infiltration of CD4+ T-cells and γδ-T-cells, combined with a lack of CD8+ T-cell infiltration and an influx of eosinophils and mast cells
In summary cluster 2 highlights the gathering pace of the pro-inflammatory response to infestation with
Cluster 3 - Six hours post infestation
Cluster 3 represents the intermediate (IM) group of genes with peak expression at 6 hpi. As in cluster 2, a number of these genes (91 of the 207 pathway mapping eligible genes in this cluster) are involved in inflammatory disease, along with "inflammatory response" (80/207), "cell-cell signalling and interaction" (79/207), "immunological disease" (81/207) and "cellular growth and proliferation" (104/207). The main canonical pathway represented here was "T-helper cell differentiation" (p = 6.38E-08), followed by the "IL10" (p = 3.72E-06), "NF-kB" (p = 1.57E-05), and "acute phase response" signalling pathways (p = 6.13E-05) and "communication between innate and adaptive immune cells" (p = 2.6E-04) (Table
Network 2 from cluster 3, showed enrichment (network score = 38) for genes involved in infectious and dermatological disease (Figure
Figure 5
IPA network depicting relationships among pro-inflammatory genes in cluster 3, 6 hours post-infestation with
IPA network depicting relationships among pro-inflammatory genes in cluster 3, 6 hours post-infestation with
Figure 6
IPA network depicting relationships among genes in cluster 3, 6 hours post-infestation with
IPA network depicting relationships among genes in cluster 3, 6 hours post-infestation with
The TNF superfamily member,
IL2 receptor alpha (
In summary, peak expression of the Th2 cytokines
Cluster 4 - Twenty four hours post infestation
Cluster 4 represents the late (L) genes with peak up-regulation at 24 hpi. The top biological function represented was "inflammatory response" with 89 of the 235 genes associated with this category. A number of additional biological functions including, "cell-cell signalling and interaction" (86/235), "cellular growth and proliferation" (111/235) and "cellular movement" (65/235) were enriched. Enriched canonical pathways included "leukocyte extravasation signalling" (p = 2.96E-05), "antigen presentation" (p = 3.9E-05), "T-helper cell differentiation" (p = 1.74E-04), "dendritic cell maturation" (p = 5.96E-04) and "IL8 signalling" (p = 3.1E-03) (Table
Another cytokine with peak expression at 24 hpi was
Figure 7
IPA representation of the canonical pathway for leukocyte extravasation signalling in cluster 4, 24 hours post-infestation with
IPA representation of the canonical pathway for leukocyte extravasation signalling in cluster 4, 24 hours post-infestation with
Figure 8
IPA representation of IgE and pro-Th2 genes in network 3 from cluster 4, 24 hours post-infestation with
IPA representation of IgE and pro-Th2 genes in network 3 from cluster 4, 24 hours post-infestation with
Cytotoxic T lymphocyte associated protein 4 (
A previous study demonstrated the involvement of MRC1 in the uptake of Der p 1 in human monocyte derived DCs
Cluster 4 contains genes involved in terminal differentiation of keratinocytes, with the small proline rich proteins (SPRRs)
The up-regulation of SPRRs in scab infected skin is likely to be controlled by the inflammatory response, either through the action of IL1, IL13, or both. The up-regulation of EDC genes at this time may aid skin barrier remodelling in an attempt to reconstruct an effective barrier against exogenous factors following mite and/or mite allergen-mediated damage. In relation to this, previous studies have highlighted the ability of HDM allergens (Der p 1 and Der p 3), for which
The S100 factors S100A9 and S100A12 (90-fold and 66-fold up at 24 hpi) represent two of fourteen S100 factors encoded for within the EDC
The increased expression of S100 genes in scab infected skin could lead to exacerbation of an already prolific inflammatory response.
In summary, cluster 4 highlights the activation and maturation of DCs, indicating that the initial pro-inflammatory response is beginning to influence an adaptive immune response to
Genes down-regulated with infestation, gene expression clusters 5-8
Cluster 5 - One hour post infestation
Cluster 5 represents the immediate early (IE) repressed genes with peak repressed expression at 1 hpi. There were insufficient genes in this cluster for effective pathway mapping. However, five genes were down-regulated at 1 hpi, namely apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2 (
Cluster 6 - Three hours post infestation
Cluster 6 represents the early (E) repressed genes with peak repressed expression at 3 hpi. The biological functions enriched in cluster 6 were "infectious disease" (6 of the 19 pathway mapping eligible genes), "connective tissue disorders" (5/19), "dermatological disease and conditions" (4/19) and "antigen presentation" (2/19). This cluster showed enrichment for the complement system (p = 5.29-12), with six complement genes (
Figure 9
IPA representation of the complement system pathway enriched in cluster 6, 3 hours post-infestation with
IPA representation of the complement system pathway enriched in cluster 6, 3 hours post-infestation with
The complement genes (
A family of inactivated serine protease paralogs, termed scabies mite-inactivated protease paralogs (SMIPPs), has been identified in the scabies mite
In summary cluster 6 highlights the reduced expression of complement genes following infestation with
Cluster 7 - Six hours post infestation
Cluster 7 represents the intermediate (IM) repressed genes with peak down-regulated expression at 6 hpi. The top biological function represented was "cancer", (78 of the 150 pathway mapping eligible genes) indicating a general down regulation in gene expression. Additional enriched biological functions included "cellular movement" (46/150), "gastrointestinal disease" (30/150) and "cellular growth and proliferation" (64/150). This cluster is dominated by genes encoding collagens, including
In summary cluster 7 highlights the reduced expression of a number of collagen genes at 6 hpi with
Cluster 8 - Twenty four hours post infestation
The final cluster of genes contained the late (L) repressed genes with peak repressed expression at 24 hpi and as with cluster 4 (24 hpi up-regulated) represents the end stage of the time course. The top biological function represented was "cancer" (60 of the 109 pathway mapping eligible genes). Additional biological functions associated with this cluster included "cell death" (44/109), "dermatological diseases and conditions" (12/109) and "cellular growth and proliferation" (45/109). As observed with cluster 4, cluster 8 was enriched for genes involved in terminal differentiation of keratinocytes with a number of genes located within the EDC locus. The most repressed of these genes were loricrin (
Down regulation of filaggrin expression is interesting from the perspective of mite proteases, in particular for the serine protease Der p 3 found in HDM extracts. A homologue of Der p 3 has been identified in
The mechanisms responsible for down-regulation of filaggrin and loricrin at this stage are unclear. However, expression of these factors, along with the involucrin (
Also down regulated (2-fold) at 24 hpi was claudin-3 (
A number of keratin encoding genes showed an approximate 2-fold down-regulation at 24 hpi. These included keratin 2 (
In summary cluster 8 highlights the importance of EDC genes and combined with the up-regulation of other EDC genes within cluster 4 (also 24 hpi), indicates an important role for this gene complex in sheep scab pathogenesis. Other roles of note within this cluster are the reduced expression of genes involved in the formation of tight junctions and also for keratin and keratin-associated genes.
This paper represents the first transcriptome level analysis of the host skin response to infestation with
Animal study
Ethical approval for this study was obtained from the Moredun Research Institute Experiments Committee and animals monitored daily in accordance with guidelines agreed with UK Home Office.
Total RNA extraction
RNA was extracted from skin biopsies following the random assignment of samples to limit batch effects. Biopsies were removed from RNA Later and excess solution and contaminating blood removed. Biopsies were dissected with a clean scalpel into smaller sections and placed into a bead homogeniser tube (2 ml CK14 type, Precellys, UK) with 1 ml of lysis buffer (Buffer RLT with β-mercaptoethanol (Qiagen, UK) and lysed with 2 cycles [6200 rpm for 23 seconds] in a bead homogeniser (Precellys 24, Precellys UK), holding on ice for 2 minutes between cycles. Lysate was cleared by centrifugation [13,000 rpm at +4°C] and supernatant transferred to a microcentrifuge tube. Total RNA was extracted with the RNeasy fibrous tissue midi-kit (Qiagen, UK) following the manufacturer's protocol with on-column DNase I digestion. RNA sample quality was assessed on an Agilent Bioanalyser (Agilent, UK), an RNA Integrity Number (RIN) was obtained for each sample and RNA yield was assessed on a ND-1000 Nanodrop spectrophotometer (Thermo Scientific, UK). RNA samples with a RIN >7.5 were considered to be of acceptable quality
Microarray study
Transcriptomic analysis was performed with the Agilent ovine gene expression microarray (15,208 ovine probes) in an 8 × 15K slide format. Samples were randomly assigned to the arrays within a slide, with all samples (time points) for a single animal hybridised onto the arrays on a single slide to limit technical variation. Total RNA was used as starting material for each array with one array used per time point (×5) (non-infected (time = 0), 1, 3, 6 and 24 hpi), per animal with a total of 30 arrays. The Agilent One-Colour gene-expression workflow (Cy3 dye, Quick Amp Labelling Protocol, Agilent, UK) was used to amplify and process the RNA samples, following the manufacturer's protocols. Briefly 800 ng total RNA was used for the generation of fluorescently labelled (Cy3 dye) complementary RNA (cRNA), using T7 RNA polymerase. Microarrays were hybridised at 65°C for 17 hours in a hybridisation oven (Agilent, UK). Microarrays were scanned on a microarray scanner (Agilent, UK) at the manufacturer's recommended settings. Microarray signal data was extracted using Agilent Feature Extraction software version 9.5.3 (Agilent, UK). To enable inter-array comparisons raw microarray data for each array was normalised to the 75th percentile and log transformed, further downstream filtering was performed in Genespring GX 11.0 (Agilent, UK).
Statistical analysis of microarray data
Differential gene expression across the time course of infestation was determined using a one way-analysis of variance (ANOVA) with a Student-Newman-Keuls (SNK) post-hoc test in Genespring GX 11.0 (Agilent Technologies, UK) comparing each of the 5 time points, non-infected (time = 0), 1, 3, 6 and 24 hpi across all animals. Multiple test correction was performed using the Benjamini & Hochberg False Discovery Rate (FDR) procedure with an FDR corrected p-value cut-off of ≤ 0.05 and a fold change cut-off of ≥2.0
Pathway analysis
Data were analysed with Ingenuity Pathways Analysis (IPA) (Ingenuity Systems,
qPCR validation
Quantitative real-time PCR (qRT-PCR) was used to verify differential expression of 11 selected genes from the final list of differentially expressed genes. Primer sets were designed as previously described (See Additional file
Additional file 2
Table S1 - Primer sequences and annealing temperatures for qRT-PCR validation of microarray data. The file contains the primer sequences and annealing temperatures for the qRT-PCR validation of the microarray data used in this study.
Click here for file
Authors' contributions
STGB designed the study, organised the research trial, performed and processed samples and microarray hybridizations, data analysis, clustering and pathway/network analysis using IPA, qPCR validations and wrote the manuscript. DF participated in the research trial and qPCR validations. FN participated in the research trial. CW participated in study design, research trial, EBI submission of the microarray data and helped prepare the manuscript. TNM contributed towards qPCR validations, data interpretation and helped prepare the manuscript. AJN participated in the study design and data analysis and helped prepare the manuscript. JFH conceived and designed the study, participated in the analysis and helped prepare the manuscript. All authors have read and approved the manuscript
This study was funded by the Department for Environment, Food and Rural Affairs (DEFRA), UK under project OD0553. The authors would like to express gratitude to Agilent Technologies UK Ltd for the ovine transcriptome microarrays and to the Division of Pathway Medicine, University of Edinburgh for help with microarray scanning. We would also like to thank Dr Belinda Norris for her helpful discussions, Moredun Clinical Division for their continued help and expertise and also Jung-Su Chang and Dr George Russell for the ovine TLR2 and TLR4 primer sets and plasmid constructs. We would also like to thank Ann Wood for the IL4 expression plasmid
History, biology and control of sheep scab
Progress and opportunities in the development of vaccines against mites, fleas and myiasis-causing flies of veterinary importance
Biology of
Examination of possible transmission of sheep scab mite Psoroptes ovis between host species
Ingestion of rabbit erythrocytes containing 51Cr-labeled hemoglobin by Psoroptes spp. (Acari: Psoroptidae) that originated on cattle, mountain sheep, or rabbits
Ultrastructure of the alimentary canal of the sheep scab mite, Psoroptes ovis (Acari: Psoroptidae)
Sheep scab: the disease, pathogenesis and control
A physiological and biochemical model for digestion in the ectoparasitic mite, Psoroptes ovis (Acari: Psoroptidae)
Confirmation of degenerative effects on psoroptic mites from scab lesions
House-dust-mite allergens
Early innate and longer-term adaptive cutaneous immunoinflammatory responses during primary infestation with the sheep scab mite, Psoroptes ovis
Dermatopathology and immunity in experimental Psoroptes ovis (Acari:Psoroptidae) infestation of naive and previously exposed Hereford cattle
Differences between primary and secondary infestations with the sheep scab mite, Psoroptes ovis
Gene expression profiling of ovine keratinocytes stimulated with Psoroptes ovis mite antigen--a preliminary study
The RIN: an RNA integrity number for assigning integrity values to RNA measurements
Cutaneous and systemic responses during primary and challenge infestations of sheep with the sheep scab mite, Psoroptes ovis
Comparison of expression profiles induced by dust mite in airway epithelia reveals a common pathway
Insights into host responses against pathogens from transcriptional profiling
DAVID: Database for Annotation, Visualization, and Integrated Discovery
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
Role of primary response genes in generating cellular responses to growth factors
Transcriptional control of the inflammatory response
Signaling to NF-kappaB by Toll-like receptors
Allergenicity resulting from functional mimicry of a Toll-like receptor complex protein
Cloning and sequence analysis of a cDNA encoding Pso o II, a mite group II allergen of the sheep scab mite (Acari: Psoroptidae)
Different doses of lipopolysaccharides regulate the lung inflammation of asthmatic mice via TLR4 pathway in alveolar macrophages
Toll-like receptor 4 (TLR4) is required for protective immunity to larval Strongyloides stercoralis in mice
Lipopolysaccharide-enhanced, toll-like receptor 4-dependent T helper cell type 2 responses to inhaled antigen
Identification of bacterial species associated with the sheep scab mite (Psoroptes ovis) by using amplified genes coding for 16S rRNA
House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells
Interleukin 25 promotes the initiation of proallergic type 2 responses
Administration of IL-33 induces airway hyperresponsiveness and goblet cell hyperplasia in the lungs in the absence of adaptive immune system
TSLP conditions the lung immune environment for the generation of pathogenic innate and antigen-specific adaptive immune responses
The novel use of the human nasal epithelial cell line RPMI 2650 as an in vitro model to study the influence of allergens and cytokines on transforming growth factor-beta gene expression and protein release
Signal transduction by tumor necrosis factor and its relatives
Cytokines and fever
Effects of selective tachykinin-receptor antagonists on tachykinin-induced airway mucus secretion in the rat
Interleukin-6 family of cytokines and gp130
House dust mite major allergens Der p 1 and Der p 5 activate human airway-derived epithelial cells by protease-dependent and protease-independent mechanisms
House dust mite, Dermatophagoides pteronissinus increases expression of MCP-1, IL-6, and IL-8 in human monocytic THP-1 cells
SOCS3 negatively regulates IL-6 signaling in vivo
SOCS-3 regulates onset and maintenance of T(H)2-mediated allergic responses
Resident skin cells in psoriasis: a special look at the pathogenetic functions of keratinocytes
The role of interleukin-1 in disease
Early events in the induction phase of contact sensitivity
Inflammatory and hyperproliferative skin disease in mice that express elevated levels of the IL-1 receptor (type I) on epidermal keratinocytes. Evidence that IL-1-inducible secondary cytokines produced by keratinocytes in vivo can cause skin disease
Enhanced production of biologically active interleukin-1 alpha and interleukin-1 beta by psoriatic epidermal cells ex vivo: evidence of increased cytosolic interleukin-1 beta levels and facilitated interleukin-1 release
Production of IL-1 alpha and IL-1 beta by human skin equivalents parasitized by Sarcoptes scabiei
Keratinocyte-derived granulocyte-macrophage colony stimulating factor accelerates wound healing: Stimulation of keratinocyte proliferation, granulation tissue formation, and vascularization
Keratins and the keratinocyte activation cycle
Modulation of cytokine expression in human keratinocytes and fibroblasts by extracts of scabies mites
The scourge of scabies
Interleukin 1 receptor antagonist is a member of the interleukin 1 gene family: evolution of a cytokine control mechanism
Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4
Biphasic production of IL-8 in lipopolysaccharide (LPS)-stimulated human whole blood. Separation of LPS- and cytokine-stimulated components using anti-tumor necrosis factor and anti-IL-1 antibodies
The biphasic mRNA expression pattern of bovine interleukin-8 in Pasteurella haemolytica lipopolysaccharide-stimulated alveolar macrophages is primarily due to tumor necrosis factor alpha
Inflammation-induced changes in the phenotype and cytokine profile of cells migrating through skin and afferent lymph
Keratinocytes from patients with atopic dermatitis and psoriasis show a distinct chemokine production profile in response to T cell-derived cytokines
House dust mite extracts activate cultured human dermal endothelial cells to express adhesion molecules and secrete cytokines
Expression of toll-like receptor 2, NOD2 and dectin-1 and stimulatory effects of their ligands and histamine in normal human keratinocytes
Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1{alpha}, and TNF-{alpha} Recapitulates Some Features of Psoriasis
House dust and storage mite extracts influence skin keratinocyte and fibroblast function
Systemic pathologic responses in experimental Psoroptes ovis infestation of Hereford calves
Sarcoptes scabiei (Acari: Sarcoptidae) mite extract modulates expression of cytokines and adhesion molecules by human dermal microvascular endothelial cells
The role of protease activation of inflammation in allergic respiratory diseases
Mite serine protease activates protease-activated receptor-2 and induces cytokine release in human keratinocytes
Interaction of mite allergens Der p3 and Der p9 with protease-activated receptor-2 expressed by lung epithelial cells
Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery
Proteolytically active allergens cause barrier breakdown
Characterisation of lesional infiltrates of dendritic cells and T cell subtypes during primary infestation of sheep with Psoroptes ovis, the sheep scab mite
Potential allergens stimulate the release of mediators of the allergic response from cells of mast cell lineage in the absence of sensitization with antigen-specific IgE
Interleukin-1-inducible genes in endothelial cells. Cloning of a new gene related to C-reactive protein and serum amyloid P component
Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune response
Biochemical and functional characterization of the interaction between pentraxin 3 and C1q
Regulation of PTX3, a key component of humoral innate immunity in human dendritic cells: stimulation by IL-10 and inhibition by IFN-gamma
IL-1 beta induces COX2, MMP-1, -3 and -13, ADAMTS-4, IL-1 beta and IL-6 in human tendon cells
Direct role of human dermal fibroblasts and indirect participation of epidermal keratinocytes in MMP-1 production after UV-B irradiation
Effects of UVR and UVR-induced cytokines on production of extracellular matrix proteins and proteases by dermal fibroblasts cultured in collagen gels%
UV-induced DNA damage initiates release of MMP-1 in human skin
Role of matrix metalloproteinases in asthma
Antioxidant enzymatic activities in human blood cells after an allergic reaction to pollen or house dust mite
Metallothionein as an anti-inflammatory mediator
The proteolytic activity of the major dust mite allergen Der p 1 conditions dendritic cells to produce less interleukin-12: allergen-induced Th2 bias determined at the dendritic cell level
Granulocyte macrophage colony-stimulating factor is overproduced by keratinocytes in atopic dermatitis. Implications for sustained dendritic cell activation in the skin
Upregulation of the release of granulocyte-macrophage colony-stimulating factor from keratinocytes stimulated with cysteine protease activity of recombinant major mite allergens, Der f 1 and Der p 1
Dysregulated activation of activator protein 1 in keratinocytes of atopic dermatitis patients with enhanced expression of granulocyte/macrophage-colony stimulating factor
Epidermal cytokine and neuronal peptide modulation of contact hypersensitivity reactions
Potential role of interleukin-10-secreting regulatory T cells in allergy and asthma
Interleukin-10 and the interleukin-10 receptor
In vivo suppression of NF-kappa B and preservation of I kappa B alpha by interleukin-10 and interleukin-13
Proteolytic cleavage of CD25, the alpha subunit of the human T cell interleukin 2 receptor, by Der p 1, a major mite allergen with cysteine protease activity
Different growth factor requirements for human Th2 cells may reflect in vivo induced anergy
The house dust mite allergen, Dermatophagoides pteronyssinus, promotes type 2 responses by modulating the balance between IL-4 and IFN-gamma
Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens
Interleukin-8 is a potent mediator of eosinophil chemotaxis through endothelium and epithelium
Synergy between proinflammatory ligands of G protein-coupled receptors in neutrophil activation and migration
Chemokines synergize in the recruitment of circulating neutrophils into inflamed tissue
Generation of anaphylatoxins through proteolytic processing of C3 and C5 by house dust mite protease
Tick-susceptible Bos taurus cattle display an increased cellular response at the site of larval Rhipicephalus (Boophilus) microplus attachment, compared with tick-resistant Bos indicus cattle
CD28 and CTLA4 coordinately regulate airway inflammatory cell recruitment and T-helper cell differentiation after inhaled allergen
CTLA-4 control over Foxp3+ regulatory T cell function
Regulatory T cells (Treg) in rheumatoid arthritis
Infestation of sheep with
Activation of dendritic cells by toll-like receptors and C-type lectins
C-type lectin receptors on dendritic cells and Langerhans cells
Involvement of the mannose receptor in the uptake of Der p 1, a major mite allergen, by human dendritic cells
Innate immune responses of airway epithelium to house dust mite are mediated through beta-glucan-dependent pathways
Innate immunity mediated by epidermal keratinocytes promotes acquired immunity involving Langerhans cells and T cells in the skin
The protease allergen Der p 1 cleaves cell surface DC-SIGN and DC-SIGNR: experimental analysis of in silico substrate identification and implications in allergic responses
Co-stimulation of T cells results in distinct IL-10 and TNF-alpha cytokine profiles dependent on binding to ICAM-1, ICAM-2 or ICAM-3
LFA-1 interaction with ICAM-1 and ICAM-2 regulates Th2 cytokine production
Fc receptors
Structural bases of Fc gamma R functions
A major house dust mite allergen disrupts the immunoglobulin E network by selectively cleaving CD23: innate protection by antiproteases
The receptor with high affinity for immunoglobulin E
The cornified envelope: a model of cell death in the skin
Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornified envelope
Interdependent transcription control elements regulate the expression of the SPRR2A gene during keratinocyte terminal differentiation
Isolation, characterization, and UV-stimulated expression of two families of genes encoding polypeptides of related structure in human epidermal keratinocytes
In vivo and in vitro SPRR1 gene expression in normal and malignant keratinocytes
Expression and regulation of small proline-rich protein 2 in allergic inflammation
Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions
The transmembrane protein occludin of epithelial tight junctions is a functional target for serine peptidases from faecal pellets of Dermatophagoides pteronyssinus
Reduction of skin barrier function by proteolytic activity of a recombinant house dust mite major allergen Der f 1
Skin barrier dysfunction and systemic sensitization to allergens through the skin
Physical mapping of a functional cluster of epidermal differentiation genes on chromosome 1q21
S100A2 coding sequence polymorphism: characterization and lack of association with psoriasis
Purification and partial characterization of a highly immunogenic human leukocyte protein, the L1 antigen
S100 protein subcellular localization during epidermal differentiation and psoriasis
S100 proteins in the epidermis
Gene (mRNA) expression in canine atopic dermatitis: microarray analysis
Calprotectin expression inhibits bacterial binding to mucosal epithelial cells
Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells
Interaction of S100A8/S100A9-arachidonic acid complexes with the scavenger receptor CD36 may facilitate fatty acid uptake by endothelial cells
Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock
The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer
Elevated serum levels of calcium-binding S100 proteins A8 and A9 reflect disease activity and abnormal differentiation of keratinocytes in psoriasis
Molecular organization and function of the complement system
Complement promotes the development of inflammatory T-helper 17 cells through synergistic interaction with Toll-like receptor signaling and interleukin-6 production
Complement regulators and inhibitory proteins
Scabies mite inactivated serine protease paralogs inhibit the human complement system
Structural mechanisms of inactivation in scabies mite serine protease paralogues
A comparative study of allergenic and potentially allergenic enzymes from Dermatophagoides pteronyssinus, D. farinae and Euroglyphus maynei
The proteinases of Psoroptes ovis, the sheep scab mite--their diversity and substrate specificity
Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite Sarcoptes scabiei
Cytokine modulation of atopic dermatitis filaggrin skin expression
Loricrin and involucrin expression is down-regulated by Th2 cytokines through STAT-6
Unravelling the skin barrier: a new paradigm for atopic dermatitis and house dust mites
Loss-of-function variations within the filaggrin gene predispose for atopic dermatitis with allergic sensitizations
Epidermal differentiation complex yields a secret: mutations in the cornification protein filaggrin underlie ichthyosis vulgaris
Breaking the (un)sound barrier: filaggrin is a major gene for atopic dermatitis
On the role of the epidermal differentiation complex in ichthyosis vulgaris, atopic dermatitis and psoriasis
The filaggrin story: novel insights into skin-barrier function and disease
Skin barrier function and allergic risk
Controlling the false discovery rate: a practical and powerful approach to multiple testing
Role of alveolar macrophages in respiratory transmission of visna/maedi virus
Molecular cloning and characterization of Toll-like receptors 1-10 in sheep


text:
Chrome Plated Atlantis Quartz Ship's Bell Clock
Chrome Plated Atlantis Quartz Ship's Bell Clock
Our most traditionally styled ship's clock, The Atlantis Chrome Bell Clock strikes every half hour in the classic ship's 8-bell cycle. The screw bezel allows easy access to the German quartz bell movement and silencer switch when the unit is mounted. The clock casing is created from chrome-plated solid, forged brass that is hand polished and flawlessly lacquered. This chrome bell clock is a beautiful addition to your boat when mounted to the bulkhead or to any office or home when displayed on one of our mahogany finished clock bases.
- Traditional ship's 8-bell cycle, rings every half hour
- Bell silencer switch
- AA battery, complete instructions and mounting hardware included
- Precision quartz movement
- Limited lifetime warranty
Dial: 4" (102 mm)
Base: 5 1/2" (140 mm)
Depth: 2 7/8" (73 mm)
- Weems & Plath


text:
Developmental Pediatrics at CHKD provides consultations for the evaluation, diagnosis and management of children with developmental disabilities.
Our program has been designated as a Child Development Center through
the Virginia Department of Health. Our full care team also consists of psychologists, social workers and educational consultants.
Note: Developmental Pediatrics at CHKD are not psychiatrists and does not provide any counseling services.
We tailor each child's treatment plan according to his or her unique needs. We may recommend a consultation with a developmental pediatrician, lab tests or other services depending on your child's assessment.
referred to
CHKD's developmental pediatrics department include premature infants, children with developmental
delays and/or genetic syndromes, children with speech and motor delays, or students failing school.
If you have questions about your bill, please call (757) 668-7200. We also offer in-depth information about insurance in our billing and insurance section.
Social work
Educational consultants


text:
OXNARD, Calif. – Sometimes being a head coach can be a lonely position.
There are so many issues that come up on a daily, if not hourly, basis. They could be player related, personnel related, coaching staff related, management related. It's part of the reason Bill Parcells liked to say, "I'm just trying to keep my own house from burning down."
Jason Garrett enters his third full season as the Cowboys' head coach with an overhauled coaching staff. Only four -- Wes Phillips, Wade Wilson, Chris Boniol and Keith O'Quinn -- remain from when he took over in the middle of the 2010 season.
Of the six new coaches on his staff in 2013, three have head coaching experience: Derek Dooley, Rod Marinelli and Monte Kiffin. Bill Callahan, who is the fourth former head coach on Garrett's staff, joined the Cowboys last year.
"I definitely believe that when you sat in that chair you have a better appreciation of what a head coach goes through," said Dooley, who headed up Louisiana Tech (2007-09) and Tennessee (2010-12) before coming in as Garrett's wide receivers coach. "For an assistant coach, when you're face with all these tough decisions as a head coach, we say, 'I would've done this or that,' and maybe you don't have the perspective he does and maybe you don't realize the four other things that affect it that might be why he made that decision. If you experience being a head coach and you can put your ego aside, you become really a better assistant. You can serve the head coach in a better capacity because you understand the challenges he's facing and understand what decisions are made and the way they are made."
Considering that Garrett may be on the hot seat after consecutive 8-8 seasons, surrounding himself with former head coaches might not be viewed as the wise thing to do. If owner and general manager Jerry Jones ever wanted to make an in-season change, he would have plenty of in-house candidates.
But Garrett feels secure in his position.
"I rely on all those guys all the time," Garrett said. "We're having constant conversations about how to do things, is this the right way, what do you think about that? And all the guys give great insight."
Marinelli was Detroit's head coach from 2006-08. Kiffin was the head coach at North Carolina State from 1980-82. Callahan was Oakland's head coach in 2002-03 and Nebraska's from 2004-07.
"You see the big picture at times," Marinelli said. "I think that's one of the benefits. You usually climb because you're good at doing certain things as a position coach or a coordinator. I think it's important that you bring that expertise to the table every day."
Garrett's decision to give up play-calling duties to Callahan was not easy. He enjoyed doing it. He was good at it. On game day, though, the hope is that he will now be freed up to better handle time management and other issues that will arise. Without play-calling duties during practice, Garrett can roam the field more to study every player and every group.
He can also interact with more players.
"There's nobody that does a better job of all the coaches I've been with than Jason does spending time and getting prepared for a team meeting or talking to players one on one," Callahan said. "He's the best at it."


text:
A Denim Shirt is a classic clothing piece to own. This one is a very versatile thing to have as this can be worn in a number of different ways to look stylish. The Del Rey Shirt is one signature shirt which should be a marvel in a womans wardrobe. This fashionable blue denim shirt is made of cotton Denim which makes it very comfortable. The shirt has a button fastening placket in front and has got roll-up sleeves which give it an all weather stylish look.
The shirt can be worn with dark jeans or can be paired up with white jeans. The double denim look can be mastered with a pair of light jeans and darker shirt or a lighter shirt with darker jeans. The shirt can be paired up with a matching skirt as well.
- Button fastening placket at front
- Roll-up sleeves
- Unlined
- Cotton Denim


text:
The Ag Education Council was formed to assist the Agriculture community in and around Fauquier County. In 2014 this council provided the first in a series of ''Winter Learning Seasons''. These sessions are specifically tailored to the agriculture business in the area and covered topics from diversification to land leasing and farm lending.
Every year in early November the Chamber hold it's Annual Meeting and Awards Gala. This committee is responsible for planning the entire event from getting bids for venues to soliciting sponsorship and silent auction items from our members. This committee meets monthly starting at the beginning of the year.
The EDL Committee meets the 2nd Monday of every month in the Chamber's conference room at 4:00pm. This committee addresses issues facing the Fauquier County and Town of Warrenton business community. This committee is in strong and consistent communication with the Town Council, County Board of Supervisors, State Delegation and our Federal Representatives. This committee recently formed a broadband sub-committee to help the county work on a county wide broadband infrastructure plan. The EDL committee plans the annual Legislative Preview and State of the County and Towns in December, Chamber Day at the Capital in late January and the Legislative Wrap Up in late April or early May.
The Education Council meets every month on the 3rd Wednesday at 4:00pm in the Chamber's conference room. This council was formed to be the bridge between our public and private schools and our business community.
The FYPC assists the young professionals in our business community. They put on educational sessions called ''Business Building Basics or B3'' covering topics such as networking, partnering and leadership. The FYPC also coordinates and plans fun events throughout the year. In May they have their annual Corn Hold Tournament and on the 2nd Thursday in September is the ever popular ''Tour of Warrenton''. The FYPC committee meets every month.
The Finance Committee is headed by our treasurer and is responsible for the consistent review of the chamber's financial status. This committee reviews the annual budget makes recommendations to the President in preparation for presentation of the new budget to the board of directors. This committee meets monthly and the date and time vary.
The Gov Con Business Council was formed to assist the government contractors in and around the Fauquier community. They launched in the fall of 2015 with a panel discussion with a prominent government contractor, a representative from SBA, our local economic development office and a representative from VEDP's department of international trade. In early 2016 they put on a very well attended symposium on how to do business with local government. They are currently planning an event focused on procurement processes with the state of Virginia followed by an event to focus on the procurement processes for our Federal Government.
The Governance and Nomination Committee is a committee of the Board of Directors, with responsibility for review of the efficient and effective practices of general governance. The GNC makes recommendations on matters to the by-laws of the organization, along with recommendations on the size, composition, members and the officers of the Board.
The Marketing Committee was formed in 2012 as the chamber was embarking on a new branding campaign. Today the Marketing committee assists the chamber in all of it's marketing endeavors. They assist in design of marketing materials (flyers, posters, ads) for the major events the chamber puts on. This committee also assist the chamber staff in website updates and marketing the chamber to non members.
The Membership Committee is responsible for recruiting, engaging and mentoring new members to the Fauquier Chamber of Commerce. Members of this committee reach out and meet with new members to provide them their plaque and start up materials and go over the many ways they can get engaged with the Chamber. The members of this committee attend luncheons, After 5 and other networking events and identify guest at the event so they can share with them the benefits of membership.
The Old Town Spring Festival is entering it's 39th year in 2017. This committee assist in the planning of this all day event. They assist in acquiring vendors and sponsors for the event and help to coordinate the 40+ volunteers needed the day of the event. This committee also helps to recruit local entertainment for all day entertainment at the Courthouse steps.
The Piedmont Harvest Fest is a 3 yr old event that was designed to merge our business and agricultural communities. The committee members are tasked with helping to recruit business, agriculture and food vendors, sponsors, and demonstrations for this all day event. There are specific members that focus on finding local talent for the entertainment component of this event.
The Valor Awards is an annual signature event of the Fauquier Chamber of Commerce. It was established in 2012 and serves to honor those individuals in the Fauquier County's Public Safety sector who have gone over and above the call of duty. The Valor Awards Committee works directly with the leaders of the Public Safety sector (Sheriff, Warrenton Police, Fire and Rescue and the State Police) to identify honoree for each year's awards. The committee members also reach out to our members and the community to solicit sponsorship to help cover the associated cost with this amazing event. This committee meets once a month starting 6 months prior to the event and the event is usually in mid to late April.
Formed in 2010, the Women's Business Council focuses on providing support to women in all facets of business - whether they are employees, entrepreneurs, mid-level administrators, or senior management. The Chamber, through the Women's Business Council, hosts educational programs and events to assist women's professional development with the ultimate goal of their success. The Executive Committee of the WBC meets quarterly to assist in the planing and review of the year's events. Membership of this committee is limited and inquiries should be directed to the Co-Chairs or Chamber staff.


text: With a severe drought, and water in San Diego countty at a premium, we are going to install a 1000 gallon underground water tank, pump, and flitration so a client can use the water on thier landscape and small fruit grove. The home is being replumbed so the gray water does not enter the sewer/septic. Herezz the question. Do any of you know of a system comes as components, be engineered, so all I have to do is connect the pieces? If not, can you suggest what ad where to start looking for? I have done a few google searches, and came up with little to no info. Thanks for the help in advance.


text:
This daily moisturiser with Essential Oils of Clove and Eucalyptus revives and re-energizes tired and dull skin. With its light, fresh texture, it helps soothe razor burn, slow down hair regrowth and protect the skin against harsh external elements. It also helps smooth fine lines and first expression lines. Use daily after shaving to revive and refresh the skin.
Free U.S. Shipping. Period. View Our Shipping Policy
Return Policy: This product is non-returnable
Return Policy: View Our Return Policy
Special Shipping Requirements: ORM-D Class
ORM-D classified products such as aerosol sprays, nail polish, perfume and products with high alcohol content may only be shipped by UPS Ground within the Continental US.
Availability: This product has been discontinued
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text:
Bear tattoo was a tattoo of the Dragon Clan which could summon the incredible strength or endurance of the bears, although not simultaneously. [1]
- ↑ Book of Earth, p. 207
|This Dragon Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
Arlen Oleson - The Knotted Cord MP3
Arlen Oleson plays a celtic tune named, "The Knotted Cord", in NYC Central on his Hammered Dulcimer. A special Thanks to David Bova for telling me the name ...
Greensleeves on the Hammered Dulcimer, Arlen Oleson MP3
Filmed in Central Park on a very cold December day in 2007.
Arlen Oleson plays MP3
in Central Park near Strawberry fields.
Arlen Olesen Tunes MP3
I play 4 tunes of Arlen Olesen.
Amazing Hammered Dulcimer Player in Central Park NYC MP3
Very talented. Performer is Arlen Oleson. This was taken just next to Bethesda Fountain under the arches. Edit (12/12/12): Album on Amazon: ...
relax in centre park with sky and clouds and arlen oleson MP3
Belemira MP3
Some of you may have seen a couple of other japanese dulcimer players have uploaded their performance on this very song. We are dulcimer friends and are ...
Mrs. Savage's Whim MP3
Another favorite that I learned from Arlen Oleson.
Musician in Central Park MP3
Musician in Central Park NYC. I would like to find out who he is. Now discovered he is Arlen Oleson.
Belemira MP3
Arlen Olesonさんのダルシマーをまねてみました。 I learned this music by a performance of Douglas Bishop & Arlen Oleson.
The Nancy MP3
Ms Savage's Whim MP3
This is also Arlen Oleson's song.
Belemira.MP4 MP3
Martine's well MP3
This song is Arlen Oleson's. I practice this now.
Belemira -clear version MP3
Another try with much lighter hammers ,wood side. Original song beautifully composed and played by Douglas Bishop & Arlen Oleson.
Epping Forest MP3
This song is Arlen Oleson's. Favorite one.
Kinlogh *soft* MP3
I learned it from Arlen Oleson's CD. Someone please tell me the meaning of "Kinlogh". Seems like it's The name of a place in Ireland ,though...
Ducky with Belemira MP3


text: Thinglink Sign up to Thinglink ThingLink - Make Your Images Interactive ThingLink lets users add interactive links to any photo and turn them into fun web experiences that drive engagement. Get started with Thinglink Tour of Thinglink Platform Tour This video shows you where to find anything you might be looking for on ThingLink.com. Learn what all the different tabs and menu options represent. Example of Thinglink Help with Thinglink Help - ThingLink Help - ThingLink


text:
Create audience
Updated: August 30, 2007
Applies To: Office SharePoint Server 2007
This Office product will reach end of support on October 10, 2017. To stay supported, you will need to upgrade. For more information, see , Resources to help you upgrade your Office 2007 servers and clients.
Topic Last Modified: 2007-08-08
Before you perform this procedure, confirm that:
You have read the topic Manage audiences.
Your system is running either the Standard or Enterprise version of Microsoft Office SharePoint Server 2007.
Important: Audiences managers must have read access to the Shared Services Provider (SSP) administration site, and must have the Manage Audiences permission enabled.
Audience members can create audiences, which can then be used by other users to target content.
Use the following procedure to create an audience.Create audience
On the Shared Services Administration home page, in the Audiences section, click Audiences.
On the Manage Audiences page, click Create audience.
On the Create Audience page, in the Properties section, type a name and description.
In the Owner text box, type or select a person to own this audience.
Select Satisfy all of the rules or Satisfy any of the rules depending on the rules you have planned for each audience.
Note: Complex rules containing AND and OR can be created by developers using the Office SharePoint Server 2007 object model.
Click OK.
Note: When you click OK, the Add Audience Rule page appears. Audiences must have at least one audience rule.
To add a rule based on a user:
In the Operand section, select User.
In the Operator section, select Reports Under to create a rule based on organizational hierarchy or select Member Of to target by group or distribution list.
In the Value box, type or select the user that you want to use to test this rule. For a Reports Under rule, select the person who is the manager of the users who you want to include in the audience. For a Member Of audience, select the group or distribution list to include for the audience rule.
To add a rule based on a property of user profiles:
In the Operand section, select Property, and then select a property from the menu.
From the Operator menu, select an operator for the property. The operators vary by property, but common operators include = and Contains.
In the Value section, type a value to use when evaluating the property against this rule.
Click OK.


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I feel the state of South Dakota has a fiscal responsibility to provide our students the opportunity to obtain a quality education. I will work hard to assure that this is achieved. A quality education system allows for our local businesses and industries to have access to a highly trained workforce. I will do my part to assure our youth have the opportunity to have a rewarding career in South Dakota and for them to raise their families and develop roots in our local communities.
Source: http://dickwerner.com/platform.html


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For sale by LEGBOROYAL VERIFIED29 Jul 2:43 pmLagos Mainland, Lagos
A new seven seater executive Sofa. ( 1 three seater, 1 two seater and 2 singles). Made of high density foam filling, wooden frame, fibre filled backrest, american weave fabric and chrome legs. Free delivery and price is negotiable. Payment on delivery within lagos.
Stay safe!
Watch out for:


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In today's Catholic Daily Roundup, top stories include the Donald's Trump failure to correct a questioner at a town hall event who incorrectly states that President Obama is Muslim, Pope Francis ask to audit the process of choosing bishops, and the Archdiocese of Washington's plan to fit 25,000 people in to Junipero Serra's canonization mass on the east portico of the Basilica of the National Shrine of the Immaculate Conception.
"Life's most persistent and urgent question is, 'what are you doing for others?'" (Martin Luther King Jr.)
Today's Reading: "For the love of money is the root of all evils, and some people in their desire for it have strayed from the faith" (1 Timothy 6:10)
In the News:
- GOP leaders continue to threaten the government with shutdown over their push to defund Planned Parenthood, and Obama becomes incresingly adament about reforms as he comes to the close of his presidency. This is a path the Republican Party's right flank has been down several times before since 2011, and they've never succeeded in achieving the policy outcome for which they began their fight in the first place. But the right swears that this time the shutdown it will be different.
- Republican presidential front-runner Donald Trump declined Thursday to correct a questioner at a town hall event who incorrectly stated that President Barack Obama is Muslim. "We have a problem in this country. It's called Muslims," said the first man Trump called on to ask a question. He continued, "We know our current president is one. You know he's not even an American." Trump, who was a driver of the "birther" movement that claimed Obama wasn't born in the U.S, first responded with feigned exasperation — "We need the question," he said, to laughs — but let the man continue.
- Migrants flooding into Croatia are being "moved on" Prime Minister Zoran Milanovic says, adding that his country cannot become a "migrant hotspot". Up to 20 buses carried migrants to the Hungary border, although Hungary says it is building a new fence there. Croatia has closed seven of its eight road crossings to Serbia after 14,000 migrants entered over the past two days seeking passage to northern Europe. Slovenia, another neighbour of Croatia, accused Croatia of breaking EU rules. The huge numbers heading north through the Balkans have triggered an EU crisis. Many are fleeing conflicts in Syria, Iraq and Afghanistan.
Pope Francis and church news:
- Pope Francis' altar for mass is steadily rising on Havana's Plaza de la Revolución they day before his trip to Cuba and then to the US, which will be the longest of his Pontificate. And looming large over that stage gazes an immense portrait of Ernesto "Che" Guevara. During his latest trip to South America, the pope made some of his strongest ever criticisms of capitalism. These remarks highlight the pope's staunch anti-capitalism, as well as an antagonism to those who advocate it. Today, almost 50 years since the death of Guevara, Pope Francis, with his barely concealed attacks on the capitalist United States, is renewing the call for global revolution.
- Pope Francis has asked his international Council of Cardinals to study the way the Church vets, identifies, and appoints bishops around the world, looking particularly at the qualities needed in a bishop today. Near the end of the council's meetings with the pope Sept. 14-16, the Rev. Federico Lombardi, Vatican spokesman, briefed reporters on its work. While one of the main tasks of the nine-member council is to assist Pope Francis with the ongoing reorganization of the Roman Curia, Lombardi said that from the beginning, Pope Francis said he wanted the group to advise him on matters of Church governance in general.
- The largest Catholic church in the Americas isn't nearly big enough to hold everyone who wants to see Pope Francis perform a Mass of canonization next week. Instead, the pope will canonize Junipero Serra from a temporary sanctuary that's being built on the east portico of the Basilica of the National Shrine of the Immaculate Conception. The church can comfortably seat 3,500 people, and up to 10,000 can cram inside on special occasions like Easter. But the pope's visit is more than that. The Archdiocese of Washington is distributing 25,000 tickets to the Mass next Wednesday.
CACG would like to equip lay Catholics with the resources necessary to promote the common good in their communities. That's why we have the Catholic daily roundup. You can sign up for this short and digestible daily e-mail here.
We always appreciate your support to continue our work into 2015. Please invest in us today.


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See preview quiet the thief outlined tight font and download this cool font for free. The font is classified as outlined tight fonts. This font viewed 79 times and downloaded 11 times. You can also downloads other fonts: Quiet The Thief Outlined Tight for Windows and Mac at FreakFonts.com. If you like this font don't forget share them with you friends!
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Carrie Holden
I bought this to try with my 11 year old cat who appears to have developed sudden allergies overnight to several things including her food. I had read lots of positive reviews on other websites about this food, so I went ahead & bought it. At first my cat tolerated it mixed with wet food, but after a week, she refuses to eat it at all. I'm left with a huge bag (the smallest available is 2.5KG on here) of expensive food that is of no use now opened. I wish companies would sell trial sized small packets of this type of food, esp for cats who are notoriously fussy to avoid a waste of money.


text: Craig Rorick is a character who appears in the CI episode Wasichu He is played by Ed Hodson. AppearancesEdit Wasichu This article is a stub. You can help the Law & Order Wiki by expanding it. Retrieved from "http://lawandorder.wikia.com/wiki/Craig_Rorick?oldid=48129" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Law & Order Wiki stubs Males CI Characters Add category Cancel Save


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The DMM Boa HMS, one of the best HMS biners around, is now available as a 3 pack of colour coded biners. The colours help simplify systems, keep things organised at belays and make it easier for teaching novice leads.
With Pre-Order, you can purchase the latest items added to our store in advance of them arriving with us.
You Pre-Order is prioritised (at no additional charge); meaning that, as soon as the product arrives with us, we can wrap it straight back up and get it sent out to you - it often never even hits the stock room shelves!
With Pre-Launch, the date when we expect to receive the product ourselves is still a little too hazy to be confident in accepting Pre-Orders.
So, instead, you can submit your email address against the item you are interested in and we will email you as soon as stock arrives on the system.
Estimated Delivery Dates are given on Pre-Order and Pre-Launch products. They are for indication purposes only and can change at any time without notice.
The dates we provide are based on indications given by our suppliers. Whenever we get updated information from our suppliers we will endeavour to update the Estimated Delivery Dates provided on product pages.
The 'Big Daddy' of lockers – the ultimate rigging biner and one which is super strong and super tough. DMM's well loved Boa HMS, one of the best rigging biners around, and its now available as a 3 pack of colour coded biners. The colours help simplify systems, keep things organised at belays and make it easier for teaching novice lead climbers.
The Boa is large enough to accommodate several knots and has a very wide gate opening – just what you need in a rigging situation. All of which makes it a great centre use biner, but the story doesn't end there.
Despite its name, the Big Boa is surprisingly light; by giving it an I-Beam construction we've reduced the overall weight by almost 25%. Add in the fact that it is easy to handle in gloves and the appeal to winter climbers is obvious.
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Fall in love!
While your kids head back-to-school for a fresh start, it's time YOU have a fresh outlook on your marriage! This season, fall more in love with your spouse with a easy to follow, in-depth program designed to inspire, connect and grow even the best of marriages!
Consider it a "Done-for-you Way to Date Your Spouse."
Our simple to follow program provides you with a weekly, printable checklist that makes date night prep easy, conversation starters done for you, intimacy tips at your fingertips and SO much more! All you have to do is click, print and prep and we've done the rest!
- Upon registration, receive a FREE copy of our A to Z Guide: 26 Ways in 26 Days to a Happier, Healthier Marriage and our Marriage Masters Text Pack, complete with over 100 texts to use with your spouse for each letter of the program!
- Every 5 weeks, throughout the duration of the program, receive additional Diva Favorites out of our virtual Diva Swag Bag, including:
- Inspirational Wall Art
- An EXCLUSIVE audio recording by licensed marriage and family therapist, Kirk Voss, equipped with free printable worksheets to ensure a full listener experience.
- An EXCLUSIVE date night designed specifically for this program that allows you and your spouse to recap and connect on strengths, weaknesses, lessons learned and more!
- A FREE Diva Store product of your choice! Yup, YOUR choice!
- At completion, receive a Marriage Masters Diploma and summary worksheets that allow you to revisit the program and "bookmark" your future goals, as a couple.
JUST $9.97 A Week!


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Framed Art Print
Girl behind the mirrorsby Diego Gomez
Choose from a variety of frame styles, colors and sizes to complement your favorite Society6 gallery, or fine art print - made ready to hang. Fine-crafted from solid woods, premium shatterproof acrylic protects the face of the art print, while an acid free dust cover on the back provides a custom finish. All framed art prints include wall hanging hardware.
This image is part digital and part manual, where an almost surreal girl emerges from a mirrored background. The intensity lies in the faces as opposed to the loose lines of the torso


text: Safeguarding 1 Safeguarding 1 p>Coming soon Search for: Clare LSP on Social Media View on FacebookClare Sports Partnership 3 days ago Clare Sports Partnership shared Clare County Council's photo. ... See MoreSee LessClare's Older Person of the Year Award PhotoView on Facebook·ShareClare Sports Partnership 4 days ago www.claresports.ie/club-rothaiochta-na-sionainne-classic/ ... See MoreSee LessClub Rothaiochta Na Sionainne Classic | Clare Sports Partnershipclaresports.ieClub Rothaiochta Na Sionainne Classic by admin | Thu 20th Jul, 2017 | General | Club Rothaiochta Na Sionainne are holding our Classic on Sunday 20th August. They have races for A3 and A4 riders. Interested? Well, here are the details. Club Rothaiochta Classic Sunday 20th August. Host Club: Club Rot... View on Facebook·ShareClare Sports Partnership 7 days ago A handy way to share your events and keep up to date with everything happening in the County. ... See MoreSee LessWhat's On In Clare Have you ever looked through your news feed and saw an event, thinking I'd like to go to that? Only to Realise it's Over. Not Anymore - That's the Plan View on Facebook·Share


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Update your Knowledge of Contact in Abaqus/Standard
Upcoming Webinar | Thursday 13th August 2015 | 3.00pm (BST) | Register Now
Contact in Abaqus/Standard has seen major developments in recent years. This webinar will walk you through these developments, the motivation behind the developments and what the current recommended best practices are. You will get a comprehensive picture of the advances made, with the help of illustrative examples. This webinar will help you:
- Understand the current best practices for contact modelling
- Learn about recent updates to contact algorithms
- Gain a better understanding of how contact works in Abaqus/Standard
About the Presenter: Dr. Haiyan Li, Senior Technical Specialist, SIMULIA Great Lakes
Haiyan Li obtained her PhD in 2003 from Department of Jet Propulsion, Beijing University of Aeronautics and Astronautics, China. Then she has worked for TsingHua University, University of Manchester and University of Minnesota, where she got a lot of Abaqus application experience in different areas, including aero engine, nuclear graphite, biomaterial and biomechanical, etc. In Dec. 2013, she joined SIMULIA as a senior technical specialist. She has expertise in structural/stress analysis with contacts, Abaqus/Explicit, fracture/failure, material constitutive models and UMAT.


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The Kenya Medical Directory website www.healthcareinkenya.com is the only healthcare portal and the most visited site in the country attracting more than 26,000 visitors a month.We have been providing this service for nearly 21 years.The two provide a friendly network which assists you to promote your products, and services hence increase your market share.
The Kenya Medical Directory online edition provides a platform which is cost effective medium of exposing your business to a specific target audience with virtual reach. The cost is comparatively less than that of advertising in the traditional media such as newspapers, television, radio and billboards.


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Sugar Creek Collection
Rely on our Double Moving Pedestal Table for all of life's special events. From first birthday parties to retirement dinners, this beautifully handcrafted pedestal table is the statement your dining room is craving.
Open your home to this classic Shaker double pedestal table and spark years of tradition, circled around in family unity and fun.
Invest in your children's future buy purchasing a dining table that will last. Make countless memories with them, and allow them to do the same with their children while feasting on family recipes around the Amish Double Moving Pedestal Table.
Be sure to check out our selection of Amish single and double pedestal dining tables to find the one that clicks with you and your family.
Make this table yours by putting 30% down today!
Questions? Comments? Please call one of our experienced Amish furniture experts at 1-866-272-6773 or visit us at our Amish furniture store on North Lockwood Ridge Road in Sarasota, Florida.
We look forward to hearing from you!
Call any of our Furniture Specialists at 1-866-272-6773 with questions.
© Copyrights 2003 - 2017 DutchCrafters All rights reserved.


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Welcome to the Muppet Central Forum!
You are viewing our forum as a guest. Join our free community to post topics and start private conversations. Please contact us if you need help with registration or your account login.
Save Muppet Central Radio
Within the week, Muppet Central Radio could be off the air. Show your support and save the station by listening via Radionomy's website and apps. We're also on iTunes and Apple TV. Learn More
Sesame Street Season 47
Sesame Street's 47th season officially began Saturday January 7 on HBO. After you see the new episodes, post here and let us know your thoughts.
The RHLC!
Discussion in 'Henson People' started by ReneeLouvier, Feb 21, 2006.
Page 411 of 415
Page 411 of 415


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QTECH-SOL PROFESSIONAL DEVELOPMENT CENTER (QPDC) is a Professional and Management Development Training Organization (NAICS 611430). We develop various Job Oriented Training Programs to individual students, looking to expand career opportunities as highly skilled professionals.
Our TRAINING division provides the best-in-class job oriented career development online training programs in Clinical Research, Pharmacovigilance-Drug Safety, Data management using SAS and Business analysis programs. Our Training programs are approved by New Jersey State - Department of Education AND NJ Department of Labor and Workforce Development. We are primarily engaged in offering an array of short duration courses and seminars towards professional development. The Training's are provided to individuals and corporate through employers training programs and to those who have been recommended and approved by NJ Workforce. Our courses are customized and modified to meet the job market. Our Instructions is provided thru our custom built Learning Management System as distance-learning (Online). The training provided has simulation methods and client settings required for student online learning.
Our PLACEMENT division offers Internship and support to client by providing resources to meet cost efficiency, time saving, and commitment towards hiring solutions to various companies in pharmaceutical, healthcare, and financial segments. QPDC is an equal opportunity employer.


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Pablo Alvaro (9th century) was a knight of Jewish ancestry from Córdoba, Spain who converted to Catholicism.
In 840, Alvaro began a correspondence with the former Bishop Bodo, a Christian convert to Judaism, wherein each tried to convince the other to return to their old faith. Many of their letters have been preserved.
See also
- Richard Gottheil and Hermann Vogelstein, Bodo Jewish Encyclopedia
- Pablo Alvaro Jewish Encyclopedia
- Corré, Alan D. The Bishop's Letter
- Epstein, Lawrence The Theory and Practice of Welcoming Converts to Judaism
This article incorporates text from the 1901–1906 Jewish Encyclopedia, a publication now in the public domain.
|This biographical article about a person notable in connection with Judaism is a stub. You can help by expanding it.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
View Submissions: last part seems to be missingAsked by Cogetil on June 11, 2015 at 02:44 AM
when i recieve the submission the last part is missing if i view it in jotform - submission viewer, but not whe copy i recieve with mail
somehow it appear now
Hello Cogetil.
It may have been a temporary connection or timeout issue that you have experienced. As the information had been emailed to you, it had also been captured as a submission and the data should be visible on the View Submissions page.
Thank you for letting us know that the issue had resolved itself on its own, which leads me to believe that it was just a form of a connection timeout or a browser cache issue.
Please let us know if the issue appears again, and we will certainly take a closer look into what might have caused it. Have a nice day.


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This week's featured coffee comes from Counter Culture Coffee. Apollo is their single origin seasonal coffee from the Idido Cooperative of Ethiopia with tasting notes of "citrus, floral, silky". Given our penchant for cold brewing bright Ethiopian coffees we had to give this one a try.
The zing of lemon zest is one of the first things we tasted followed by smooth lingering notes of orange flavored chocolate. A perfect cold brew to drink in the afternoon over ice. If you like bright coffees, we definitely recommend giving this one a try.


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Would you like to save as your currency permanently or for this visit only?
early closing of restaurant
easy to find and although we arrived late arrangements had been made for us to get into our rooms No for what we wanted it was ideal
The room was comfortable and a good size for a family of 4. the bathroom was clean and the shower was good.
free wifi. good room and bathroom. quiet. if you dont have a car you cannot stay here. it is kilometres away from the city centre and practically no public transport. No lift available.
The staff were both friendly and helpful The entire reception/admin closed at 7pm
Near highway and Angers The window of the rom was near the highway
The room was large and pleasant. The continental breatfast was reasonable. The Internet did not work - they use Noodo Wifi, I could not get this to work. When I tried to register it, it said I was already registered. It would give me passwords that did not work and would say I was not registered. In other hotels, the Internet works fine and I have no trouble. The Internet is an important consideration for me in the choice of a hotel.
The manager was very hospitable. The rooms were clean and conveniently located. Breakfast was very expensive.
Able to have an adjoining room for kids, and also kids eat free at breakfast. There is a Buffallo Grill 5 minute walk away. Located in an industrial estate, need a car to access anything. Daunting walk back in the evening from the Buffalo Grill as the hotel was in darkness as if it was closed down.
easy to find, rooms well laid out and comfortablewe we selected the hotel because it had a resteraunt on site, after traveling all day we did not want to have to go out again for a Meal. On arrival we were told the restaraunt was not open , no explanation .we had to eat out at a Bufalo Grill, we were very disappointed!
It's quiet and has a fine restaurant. There's a bus stop about 200 metres away and could take you to the city centre within 20 minutes. It's not that convenient if you travel by public transports. There's not any service later than 7:30pm although the bus stop is just 2 minutes away.
Simplicity, you kno wwhat to expect at a Campanille
Friendly stuff, easy booking, no englsih speaking TV station. At least one english speacing tv station should be available
Too near to other traffic and to other hotels Not eay to find. I think it would have been incredibly for a non-French speaker to find. More directions are needed
a tea kettle in the room
Restaurant on site aftera day travelling was very helpful and enjoyable, good value meal.
Easy and quick access to rooms from parking Noisy street, bad flushing of shower
Ease of finding hotel. Excellent food in the Restaurant and plenty of choice for breakfast. Room clean and had everything we required with access to garden. Very good value for money. No
Restaurant excellent. Staff helpful and friendly. Would certainly consider staying in this hotel again if in the area.
It was fairly calm, the bed was good. The staff present were not too well informed, however quite friendly.Not so good the fact that one should specify that 3 people in 1 room are not necessarily a couple plus 1 single! So 3 male guests find themselves with a french bed with one duvet plus an extra bed. This should be clearer in the room description.
nice room, good restaurant friendly staff no
Cleanliness, friendliness of staff, location. Not exactly a bad point but I thought dinner was ordinary.
Staff were very accommodating. Restaurant was very good.
Quiet,comfortable,pleasant service and attention to detail.Food was varied and well presented.Definitely a hotel we would use again. A small fault was the height of the ceiling above one side of the bed which,due to the sloping roof was a bit low for a tallish person such as me.
the cleanliness and the breakfast
Facilities good. Hotel was almost empty - only about 6 other guests. Restaurant was closed and we were given a meal for E25, which was rather poor. I could not eat most of it and was given terrine, tomato salad, and 3 pieces of fruit for E25!
Convenient, practical, clean and friendly staff. No. We merely slept there for two nights whilst visiting other locations.
All fine, very friendly, and very helpful. Lack of English speaking staff made things interesting, however the staff went to great lengths to assist in communication.
nice people, helpfull, good resturant, they have 1/2 bord price very good by booking with them directly
The room was very comfortable, with a balcony overlooking the river. The food in the restaurant was excellent and breakfast was good. It is ideally situated for anyone cycling the Loire and had secure cycle storage.
Very tranquil setting overlooking the Loire. Friendly and helpful staff. Excellent food. Menu du jour was very good value.Delightful decor.
Brilliant! Stayed a week and used it as a base to explore the Loire. Lovely view out the window in the mornings. Very filling continental breakfast for €8. Wonderful food in the restaurant - tried to return in time for it each evening. Lots of room, good shower facilities etc. Plenty of parking. All very friendly. Excellent all round!
Location on banks of Loire, calm. No restaurant on Sunday or Monday
A really nice little hotel, which we recommend. Although the hotel was cheap, it was very clean, had all the facilities we wanted, including touches like hairdrier, lots of towels and hot water etc. and the staff were friendly. Although some of the staff only spoke French, it was nice to be in a proper French hotel, rather than an 'international' one. It had the Seine the other side of the road, and we had a lovely view of this from our rooms and restaurant, as well as a lake with a playground on the bank. There was a good choice of evening meal, and the breakfast was really enjoyable and extensive. no
Good fiarly easy to find some restraunts close by. Usual small accomodation for Premier Class but more than adequate for ovewr night stay. Breakfast Area cramped.
It was comfortable but their was no food available in the evening fortunately their was resturants near by We are not use to animals when we eat our food
Quite efective place. Just to have a clean room to sleep. Good (value for money) breakfast very small rooms and toilet
Friendly staff and good breakfast. Rooms were gloomy
We received what we expected. The position at the outscirts of the city turned out to be advantagous (e.g. no problems with parking).
Good cheap basic accommodation Room very warm at night
The staff spoke good English The layout was confusing. There needed to be better signage when you come in first.
The receptionist on our arrival was very good. The swimming pool was good The other receptionist we had dealings with was unhelpful one example- she said there was no need to book for dinner that night and consequently we went hungry as it was a monday. There did not seem to be a map of the resort/complex (if there was we were not given one) and the sign posts were useless. There was no information in english- re attractions etc in the area.
it was very souless (for a business traveller)
beds very comfortable ,clean,and a heating if required.Staff excellent. a little hard to find,but turn right into carpark at La Pommeraye signpost coming from Montjean
It was very well located in a nice, tranquil area. Nothing
The shower was good. The hotel had no air con and had to open the patio door to a shared balcony with smokers. The room was very outdated and we had several biting flies. We actually checked out four days early and moved to a better hotel.