text:
Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation
Mechanisms that confer an ability to respond positively to environmental osmolarity are fundamental to ensuring embryo survival during the preimplantation period. Activation of p38 mitogen-activated protein kinase (MAPK) occurs following exposure to hyperosmotic treatment. Recently, a novel scaffolding protein called Osmosensing Scaffold for MEKK3 (OSM) was linked to p38 MAPK activation in response to sorbitol-induced hypertonicity. The human ortholog of OSM is cerebral cavernous malformation 2 (CCM2). The present study was conducted to investigate whether CCM2 is expressed during mouse preimplantation development and to determine whether this scaffolding protein is associated with p38 MAPK activation following exposure of preimplantation embryos to hyperosmotic environments.
Our results indicate that
These results define the expression of upstream activators of p38 MAPK during preimplantation development and indicate that embryo responses to hyperosmotic environments include elevation of CCM2 and activation of p38 MAPK.
Culture medium osmolarity is one of the primary parameters that must be considered when formulating an optimized medium for the production of preimplantation embryos. Even brief exposure of preimplantation embryos to 300 mOsm/kg culture media (in the absence of osmolytes) results in impaired development
MAPKs function by propagating extracellular signals via a series of phosphorylation events through sequentially arranged protein kinases, resulting in cellular responses ranging from transcriptional to post-translational events
The discovery of a class of compounds called cytokine-suppressive anti-inflammatory drugs (CSAIDs) has allowed for the specific pharmacological inhibition of MAPK14/p38α and MAPK11/p38β isoforms
Among its possible roles in the early embryo, p38 MAPK signaling is likely to mediate embryonic responses to hyperosmotic stimuli. Recently, a novel scaffolding protein called Osmosensing Scaffold for MEKK3 (OSM) was characterized
Figure 1
CCM2 as a Scaffold for p38 MAPK Activation
CCM2 as a Scaffold for p38 MAPK Activation. As described in [26] the Rho-GTPase, RAC, is recruited to actin membrane ruffles by CCM2 following hyperosmotic stress, facilitating the activation of p38 MAPK. CCM2 acts as a scaffold binding to RAC, MAP3K3, and MAP2K3 to organize these components into a functional signaling module. We have included in this model the two additional upstream MAP2Ks to p38 MAPK: MAP2K6, a specific activator of p38 MAPK related to MAP2K3; and MAP2K4, which primarily activates the JNK/SAPK pathway, but can also phosphorylate p38 MAPK
The present study was conducted to investigate whether CCM2 and upstream p38 MAPK pathway constituents are expressed during preimplantation development and to determine whether changes in CCM2 expression are associated with p38 MAPK activation following exposure of preimplantation embryos to hyperosmotic stimuli. Our results indicate that CCM2 is expressed throughout mouse preimplantation development and its protein levels are elevated in response to hypertonic culture conditions and correlate with increased p38 MAPK activity in the early embryo.
Detection of mRNA transcripts encoding
Qualitative RT-PCR methods resulted in the detection of mRNA transcripts encoding
Figure 2
Detection of mRNA Transcripts encoding p38 MAPK Pathway Constituents and CCM2 during Mouse Preimplantation Development
Detection of mRNA Transcripts encoding p38 MAPK Pathway Constituents and CCM2 during Mouse Preimplantation Development. RT-PCR products encoding
Relative abundance of mRNAs encoding
Cellular distribution of CCM2, MAP3K3, phosphorylated MAP2K3/MAP2K6 and phosphorylated MAP2K4 proteins during mouse preimplantation development
CCM2 immunofluorescence was detected in all stages of early mouse development, from the 1-cell to the blastocyst stage (Figure
Figure 3
Immunofluorescence Detection of CCM2, MAP3K3, Phosphorylated MAP2K3/6 and Phosphorylated MAP2K4 during Mouse Preimplantation Development
Immunofluorescence Detection of CCM2, MAP3K3, Phosphorylated MAP2K3/6 and Phosphorylated MAP2K4 during Mouse Preimplantation Development. Whole-mount indirect immunofluorescence of 1-cell, 2-cell, 4-cell, 8-cell, morula, and blastocyst stage mouse embryos with antisera raised against CCM2 (CCM2), MAP3K3 (MAP3K3), phosphorylated MAP2K3/MAP2K6 (P-MAP2K3/6) and phosphorylated MAP2K4 (P-MAP2K4). CCM2 and MAP3K3 immunofluorescence was distributed diffusely throughout the cytoplasm of each blastomere in all embryo stages. Phosphorylated MAP2K3/MAP2K6 (phosphorylated at Ser189/107) immunofluorescence was detected in the cytoplasm and nucleus of each blastomere in each embryo stage. Phosphorylated MAP2K4 (phosphorylated at Thr261) immunofluorescence was primarily localized to the nucleus of each blastomere in each embryo stage. In all confocal micrographs, green indicates positive staining for specific protein and red indicates F-actin (rhodamine-phalloidin). Scale bars represent 50 μm.
Effect of culture in 1800 mOsm hyperosmotic medium on blastocyst morphology and p38 MAPK activation
We initially employed treatment with high culture osmolarity (i.e. 1800 mOsm) to explore the effects of an extreme treatment paradigm on phospho-MAPKAP2 fluorescence. Blastocyst culture in KSOMaa media containing 10% glycerol or 1.4 M sucrose resulted in an instantaneous decrease in blastocyst volume. Those placed in medium + 10% glycerol displayed a rapid recovery (5 minutes) back to normal volume, however, this recovery was not observed in embryos placed in 1.4 M sucrose medium (data not shown). Phospho-MAPKAPK2 immunofluorescence increased in blastocysts cultured in KSOMaa + 1.4 M sucrose for 10 minutes (Figure
Figure 4
Immunofluorescence Detection and Quantitation of Phosphorylated MAPKAPK2 Following Culture in 1800 mOsm Medium for 10 Minutes and 30 Minutes
Immunofluorescence Detection and Quantitation of Phosphorylated MAPKAPK2 Following Culture in 1800 mOsm Medium for 10 Minutes and 30 Minutes. Phosphorylated MAPKAPK2 was detected in the cytoplasm and the nucleus of all embryos observed following 10 minutes and 30 minutes of treatment. There was a noticeable increase in the level of phosphorylated MAPKAPK2 protein detected in blastocysts cultured in KSOMaa + 1.4 M sucrose (C, G) when compared with blastocysts cultured in KSOMaa + 10% glycerol (B, F) or KSOMaa only (A, E) at both 10 minutes and 30 minutes of treatment. No primary controls are shown (D, H). All confocal micrographs are representative images from pools of blastocysts in each treatment group. Green indicates positive staining for MAPKAPK2 proteins (when phosphorylated at Thr334), red indicates F-actin (rhodamine-phalloidin) and blue indicates nuclei (DAPI). Scale bars represent 50 μm. Scion Image analysis applied to FITC immunofluorescence images representing detectable phosphorylated MAPKAPK2 at 10 minutes (I) and 30 minutes of treatment (J). Relative signal strengths are presented as the mean ± s.e.m. representative of three independent replicates. Bars with different letters represent significant differences in relative signal strength between treatment groups (
Effect of 460 mOsm hyperosmotic treatment on blastocyst morphology and p38 MAPK activation
To investigate the effects of reduced levels of hyperosmolarity on phospho-MAPKAPK2 fluorescence we next conducted the following experiment using culture medium adjusted to 460 mOsm. Exposure of cultured blastocysts to a 1.4% glycerol or 0.2 M sorbitol medium did not visibly affect blastocyst volume (data not shown). While no perceptible differences in blastocyst volume were observed between embryos following 15 minutes and 30 minutes of 460 mOsm hyperosmotic treatment, variations in the levels of phospho-MAPKAPK2 fluorescence were detected (Figure
Effect of 460 mOsm hyperosmotic treatment on
To explore a possible mechanism for the induction of p38 MAPK following incubation in hyperosmotic medium we next examined the influence of exposure to hyperosmotic medium on
Figure 5
Effect of 460 mOsm Hyperosmotic Treatment on
Effect of 460 mOsm Hyperosmotic Treatment on
Our overall objective was to increase our understanding of the intracellular signaling pathways that respond to the external environment and regulate preimplantation development. Our recent characterization of the expression of all four isoforms of p38 MAPK (MAPK14/p38α, MAPK11/p38β, MAPK12/p38γ, and MAPK13/p38δ) and a number of its downstream substrates provided the foundation for our current studies
Each gene of interest displayed a consistent increase in transcript abundance as embryo development advanced towards the blastocyst stage. There was a significant increase in relative mRNA levels from the 8-cell stage to the morula and blastocyst stages for all transcripts investigated. These results suggest that mRNAs for p38 MAPK pathway constituents are rapidly accumulating in the post 8-cell stage embryo and this observation correlates well with outcomes from p38 MAPK inhibition studies which suggest that p38 MAPK signaling is required to maintain development beyond the 8–16 cell stage
At the protein level, the CCM2 and MAP3K3 cytoplasmic distribution pattern observed following application of whole-mount indirect immunofluorescence was as expected considering the role of CCM2 as an actin binding scaffold protein and MAP3K3 as an upstream MAP3K that is responsive to extracellular stimuli at the cell surface. The diffuse cytoplasmic distribution mirrors the localization pattern displayed by these proteins in HEK293 and COS7 cell lines
Since it is well known that the roles played by p38 MAPK extend well beyond the regulation of the actin cytoskeleton our present study investigated whether embryonic p38 MAPK signaling is affected by exposure to hyperosmotic stimuli as is reported for somatic cells. Our results clearly demonstrate that hyperosmotic treatment during culture increased p38 MAPK activity as assessed by increased phosphorylation of its specific downstream substrate, MAPKAPK2. These findings are supported by numerous studies that have described the responsiveness of this pathway to hyperosmotic stress, and have indicated that increases in p38 MAPK activity are a common cellular response to hyperosmotic stimuli (reviewed by
At both 1800 mOsm and 460 mOsm hyperosmotic treatment conditions, the addition of glycerol to KSOMaa culture medium did not result in a significant increase in phospho-MAPKAPK2 immunofluorescence in blastocysts. This is in contrast to the 1.4 M sucrose (1800 mOsm) and 0.2 M sorbitol (460 mOsm) treatments in which a significant increase in phospho-MAPKAPK2 immunofluorescence was detected at two treatment time-points. We propose that this difference in p38 MAPK activation is a result of the alleviation of osmotic stress and restoration of osmotic equilibrium across the cell membrane due to aquaporin (AQP; water channel) mediated glycerol permeability. This likelihood is supported by our previous studies which demonstrated the selective permeability of water and glycerol, but not sucrose, through apical and basolateral AQPs located in the blastocyst trophectoderm
Interestingly, hyperosmotic treatment of cultured rat astrocytes by mannitol or sorbitol increases the expression of AQP4 and AQP9 mRNAs and proteins, which is suppressed by treatment with the p38 MAPK inhibitor SB203580
By quantifying relative signal strength of phospho-MAPKAPK2 immunofluorescence, we observed a 2-fold increase in p38 MAPK activity following 1.4 M sucrose treatment for 10 minutes and a 1.75-fold increase following 0.2 M sorbitol treatment for 15 minutes. A 2-fold increase in p38 MAPK activity is consistent with results observed in other studies
Though changes were not detected in localization or steady state mRNA transcript levels of CCM2 following hyperosmotic treatment, our results did reveal a significant increase in CCM2 immunofluorescence in blastocysts treated for 15 minutes in 0.2 M sorbitol (460 mOsm), but not 1.4% glycerol (460 mOsm). These results suggest a solute specificity in the induction of CCM2, which agrees with the induction of p38 MAPK activity and phosphorylation of MAPKAPK2 described above. Uhlik et al.
In conclusion, we have demonstrated for the first time that transcripts and polypeptides encoding MAP3K3, MAP2K3, MAP2K6, MAP2K4 and CCM2 are expressed and localized throughout mouse preimplantation development. We have discovered that p38 MAPK activity is regulated by exposure to hyperosmotic stimuli, and that the response to hyperosmotic stress in the early embryo includes increased CCM2 levels. These outcomes provide a basis for understanding the mechanisms controlling osmotic induction of p38 MAPK activity during preimplantation development. The outcomes therefore further our knowledge of the regulation of intracellular signaling pathways within the mammalian embryo and understanding of how culture environments can affect embryo development. The preimplantation embryo is highly sensitive to the environment in which it develops. With the extension of embryo culture to the blastocyst stage emerging as a routine treatment paradigm in human infertility clinics, it becomes even more important that we study the short-term and long-term effects of culture on embryo development. Our results may therefore contribute to advancements in the development of improved embryo culture systems with the ultimate goal of increasing our ability to produce healthy embryos for embryo transfer.
Superovulation and mouse embryo collection
Female CD-1 (6–8 weeks of age, Charles River Laboratories, Saint-Constant, QC, Canada) or MF-1 mice (4–5 weeks of age, Harlan Sprague Dawley, Indianapolis, IN, USA) were superovulated by intraperitoneal (IP) injection of 10 IU pregnant mare serum gonadotropin (PMSG; Intervet Canada Ltd., Whitby, ON, Canada) followed 48 hours later by 10 IU human chorionic gonadotropin (hCG; Intervet Canada Ltd., Whitby, ON, Canada) prior to mating with CD-1 males (Charles River Laboratories, Canada) as described
RNA extraction
Total RNA was extracted from pools of 20 or 40 preimplantation stage mouse embryos or positive control tissue (mouse kidney). Samples were frozen and stored at -80°C in 500 μl of TRI Reagent® (Molecular Research Center Inc., Cincinnati, OH, USA) with 5 μL of Polyacryl Carrier (Molecular Research Center Inc., Cincinnati, OH, USA) following collection. In samples used for quantitative Real-Time reverse transcription and polymerase chain reaction (RT-PCR) analysis, 0.025 pg/embryo of exogenous Luciferase Control RNA (Promega Corporation, Madison, WI, USA) containing a 30-base poly(A) tail was added to each pool of embryos prior to RNA extraction.
Reverse Transcription and Polymerase Chain Reaction (RT-PCR)
Embryo total RNA was reverse transcribed (RT) using Random Primers (Invitrogen Life Technologies, Burlington, ON, Canada) and RNaseOUT™ Ribonuclease Inhibitor (Invitrogen Life Technologies, Burlington, ON, Canada) along with Sensiscript RT (Qiagen Inc., Mississauga, ON, Canada) according to the manufacturer's suggested protocol. Following one hour incubation, the sample was diluted to a concentration of 1 embryo equivalent per μL (embryo/μL) and subjected to PCR amplification of H2A histone family, member Z (
Table 1
PCR Primer Sequences for Amplification of
Gene product
Primer Sequence
Size (bp)
Ensembl Transcript ID
Custom TaqMan® gene expression assay design for real-time PCR
The Custom TaqMan® primer and probe sets for
Quantitative real-time PCR analysis
Real-Time PCR reactions were performed using the ABI PRISM® 7900HT sequence detection system (Applied Biosystems, Foster City, CA, USA) and TaqMan® Gene Expression Assays (Applied Biosystems, Foster City, CA, USA). Pre-designed and pre-optimized commercially available TaqMan® Gene Expression Assays for MEKK3/Map3k3 (Assay ID: Mm00803725_m1), MKK3/Map2k3 (Assay ID: Mm00435950_m1), MKK6/Map2k6 (Assay ID: Mm00803694_m1), MKK4/Map2k4 (Assay ID: Mm00436508_m1), p38α/Mapk14 (Assay ID: Mm00442497_m1), were used along with the Custom TaqMan® Gene Expression Assays described above for
Relative quantification of target gene expression levels was performed using the comparative CT (threshold cycle) method (ABI PRISM® Sequence Detection System, version 2.1, Applied Biosystems, Foster City, CA, USA). The quantification was normalized to the control luciferase RNA levels
Rabbit antisera raised against the phosphorylated forms of phospho-MKK3/MKK6 (Ser189/207), phospho-SEK1/MKK4 (Thr261), and phospho-MAPKAPK2 (Thr334) were used (Cell Signaling Technologies Inc., Danvers, MA, USA). The anti-MEKK3 antiserum used was a mouse monoclonal antibody (BD Biosciences Pharmingen, San Diego, CA, USA). The rabbit polyclonal anti-OSM/CCM2 antiserum was a gift generously donated by Dr. Gary L. Johnson (University of North Carolina at Chapel Hill)
Whole-mount indirect immunofluorescence and confocal microscopy
Mouse preimplantation stage embryos were collected and processed for application of whole-mount immunofluorescence as described
Image analysis and quantification of immunofluorescence intensity
To quantify immunofluorescence results we employed the method developed and reported by
The Scion Image program, Version 184.108.40.206 (Scion Corporation, Frederick, MD, USA), is a freely distributed commercial software program that mimics the performance of NIH Image program for quantification of the chromogen signal strength
Hyperosmotic treatment of cultured blastocyst stage mouse embryos
Eight cell stage mouse embryos were flushed from the oviducts of timed-pregnant female mice, washed, pooled in groups of 20, and cultured in 20 μL drops of KSOMaa until the blastocyst stage (approximately 90–95 hours post-hCG for cultured embryos) prior to hyperosmotic treatment. Culture medium osmolarity was adjusted by the addition of glycerol, sucrose, or sorbitol to KSOMaa embryo culture. Treatment groups and their respective osmolarities were: (
Statistical analysis
Statistical analysis was performed using SPSS®, Version 14.0 (SPSS Inc., Chicago, IL, USA) or SigmaStat® 2.0 (Jandel Scientific Software, San Rafael, CA, USA) software packages. Real-Time PCR results are presented as the mean ± s.e.m. for relative mRNA transcript levels from three independent replicates. Real-time RT-PCR data were square-root transformed, subjected to one-way analysis of variance (ANOVA), and followed by Tukey's Multiple Comparison Test or non-parametric Mann-Whitney Rank Sum Test. Results from Scion Image analysis are presented as the mean ± s.e.m. for relative signal strength (RSS) from three independent replicates with the number of embryos in each treatment group indicated. All data was tested for homogeneity of variances by Levene's Test for Equality of Variances. In instances of equal variance, data was subjected to one-way ANOVA followed by Fisher's Least Significant Different (LSD) test for comparing three means of unequal group size. When equal variances were not observed, data was subjected to Welch's variance-weighted ANOVA followed by the Games-Howell (GH) Post-hoc Test for Multiple Comparisons, appropriate for situations of unequal (or equal) sample sizes and unequal or unknown variances. For all data analysis,
Authors' contributions
BF participated in the design of the study, performed the PCR, immunofluorescence and hyperosmotic response experiments and drafted the manuscript. PHW assisted in conducting the Real-Time RT-PCR analysis, experimental design, analysis of outcomes and provided critical review of the results and manuscript contents. AJW directed the study, provided critical review of the results, co-authored the manuscript and acquired funding for the research program. All authors read and approved the final manuscript.
Our gratitude is extended to Dr. Pavneesh Madan, Dr. Michele Calder, Julie Andrassy, Andrew Paliga, Anne Pin, Michelle Violette, and Christine Witchell for their expertise and assistance with embryo collections. We are especially grateful to Dr. Gary L. Johnson, University of North Carolina at Chapel Hill for providing us with the rabbit polyclonal anti-OSM/CCM2 antiserum used in this study. This research was supported by an operating grant from the Canadian Institutes of Health Research (CIHR) to AJW.
The protective action of betaine on the deleterious effects of NaCl on preimplantation mouse embryos in vitro
Organic osmolytes and embryos: substrates of the Gly and beta transport systems protect mouse zygotes against the effects of raised osmolarity
Glycine protects preimplantation mouse conceptuses from a detrimental effect on development of the inorganic ions in oviductal fluid
Osmoregulation and cell volume regulation in the preimplantation embryo
Betaine is a highly effective organic osmolyte but does not appear to be transported by established organic osmolyte transporters in mouse embryos
Regulation of intracellular glycine as an organic osmolyte in early preimplantation mouse embryos
The glycine neurotransmitter transporter GLYT1 is an organic osmolyte transporter regulating cell volume in cleavage-stage embryos
p38 kinase activity is essential for osmotic induction of mRNAs for HSP70 and transporter for organic solute betaine in Madin-Darby canine kidney cells
MAP kinases and the adaptive response to hypertonicity: functional preservation from yeast to mammals
Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation
A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells
Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta)
The primary structure of p38 gamma: a new member of p38 group of MAP kinases
Characterization of the structure and function of the fourth member of p38 group mitogen-activated protein kinases, p38delta
Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human
Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms
Characterization of the structure and function of a novel MAP kinase kinase (MKK6)
MAPKAP kinase-2; a novel protein kinase activated by mitogen-activated protein kinase
PRAK, a novel protein kinase regulated by the p38 MAP kinase
Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases
A protein kinase involved in the regulation of inflammatory cytokine biosynthesis
Pharmacological effects of SB 220025, a selective inhibitor of P38 mitogen-activated protein kinase, in angiogenesis and chronic inflammatory disease models
p38 MAPK signaling during murine preimplantation development
p38 mitogen-activated protein kinase (MAPK) first regulates filamentous actin at the 8-16-cell stage during preimplantation development
Requirement of the MAP kinase signaling pathways for mouse preimplantation development
Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock
CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis
Activation mechanism of c-Jun amino-terminal kinase in the course of endodermal differentiation of P19 embryonic carcinoma cells
Induction of c-fos expression through JNK-mediated TCF/Elk-1 phosphorylation
Dual roles for c-Jun N-terminal kinase in developmental and stress responses in cerebellar granule neurons
Rac-1 and IQGAP are potential regulators of E-cadherin-catenin interactions during murine preimplantation development
Aquaporin proteins in murine trophectoderm mediate transepithelial water movements during cavitation
Channel-dependent permeation of water and glycerol in mouse morulae
Hyperosmolar mannitol simulates expression of aquaporins 4 and 9 through a p38 mitogen-activated protein kinase-dependent pathway in rat astrocytes
Osmotic stress up-regulates aquaporin-3 gene expression in cultured human keratinocytes
Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene
Functional challenge affects aquaporin mRNA abundance in mouse blastocysts
The osmoregulatory and the amino acid-regulated responses of system A are mediated by different signal transduction pathways
Hypo-osmotic cell swelling activates the p38 MAP kinase signalling cascade
Hypertonicity activates MAP kinases and inhibits HCO-3 absorption via distinct pathways in thick ascending limb
Na+/K+ -ATPase regulates tight junction formation and function during mouse preimplantation development
An improved culture medium for mouse blastocysts
Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression
Comparative analysis of development-related gene expression in mouse preimplantation embryos with different developmental potential
Application of Photoshop and Scion Image analysis to quantification of signals in histochemistry, immunocytochemistry and hybridocytochemistry


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Dyckia Species
Dyckia is a genus of 120 terrestrial bromeliads. Dyckia are striking rare plants native to central South America, primarily Brazil.
Dyckia plants are related to the pineapple and easy to grow. They like well-drained soil and enjoy ample water in summer. For pot culture, they like to have room to allow for root growth. Dyckia plants love exposed full sun positions in a garden.
Dyckia have stiff and usually spiny leaves. Their native habitats are often rocky. Most Dyckia species grow in a clumping habit, often forming large mats. Dyckia flowers emerge from the side of the leaf rosette and are usually yellow to orange.
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subset: commoncrawl


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A recipient of Frost & Sullivan's 2012 North American Product Leadership Award in Disinfection Equipment for Shale Oil and Gas Wastewater Treatment and chosen as a 2013 IHS CERAWeek Energy Innovation Pioneer, Ecosphere has enabled oil and gas customers to recycle and reuse over 2.8 billion gallons of water on more than 700 oil and natural gas wells in major shale plays around the United States.For more information, please visit www.EcosphereTech.com . To receive timely information on Ecosphere Technologies, sign up for Ecosphere's email news alert system at http://www.ESPH-IR.com . The Ecosphere Technologies, Inc. logo is available at http://www.globenewswire.com/newsroom/prs/?pkgid=12214
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Judging for National History Day in Indiana
Thank you for volunteering to judge for a National History Day in Indiana contest. Your contribution to the program is part of what makes NHD unique and provides a valuable role in the authentic learning experience for these students. In preparation for the event, please take a few minutes to review the information below. You'll receive hard copies of these materials on the day of the contest.
NHDI Contest Page for general contest information
National History Day Rulebook
2014 Theme Sheet: Rights and Responsibilities in History
Judging tip sheet
Please arrive at the contest's judges registration room before 8 a.m. on the day of the contest to meet your fellow judges and get instructions for the day. Coffee and snacks will be provided at each orientation, and lunch will be provided for all volunteers following morning judging.
North District Contest
St. Mary's College, February 15
Judging orientation in Student Center
Park in parking lot next to Student Center.
Central District Contest
IUPUI, March 15
Judging orientation in Lecture Hall 101
Park in visitor's parking garage marked 'XB' on the linked map and get parking validated at lunch.
South District Contest
Brown County High School, April 12
Follow signage to find judges' room.
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Marian University, May 3
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Home Business Tips, Tricks, And Techniques For Success
If you have always wanted to work in your pajamas, then you should look into owning and operating a home based business. While it's not a get rich quick scheme, (you will have to do lots of hard work) it can be a way to break out of your dependence on an employer and make a living on your own. Use the tips here to get started.
Before starting a home business, make sure you are ready to take on the responsibilities that come with it. Many people find their business unsuccessful because they did not have the time or resources to invest in it. Also, make sure you have time to deal with home business responsibilities.
Attracting clients to your home business is integral to its success. Social media because of its popularity can be a critical medium for advertising and marketing your business. Your business should have its Social Media feed. You can also find creative ways to market your business on websites like Reddit and Digg. Finally, since you are working from home and likely using the same computers for both personal and business use, make sure to keep the accounts separate!Surely, you may have learned to avoid sites like
which a horrible suggestions.
Seek out experts in your field when you have questions. If you are nervous about calling them, send them an e-mail. It's important to be able to model someone who is a success, and people who have made themselves successful are usually willing to help beginners out. This will save you years of mistakes.
Before you start selling anything, you should make sure to find out what the price range for it is in the current market. Price the item or service as offered by your competitors, and aim your price just slightly lower. Talking negatively about another business is bad for your own, so focus on making your good qualities stand out instead.
If you have a home business, advertise by posting flyers on your local community bulletin board, passing out flyers in your neighborhood, or taking out a classified ad in an online advertiser for your region. These methods are free or low-cost and they are good in getting the word out about your business.
Ensure that your home business is properly insured, by discussing the business with an insurance agent. You should also make sure that your home business complies with local zoning laws and city ordinances. You don't want to start a dog care business, if there is a limit to the amount of animals you can have on your property.
Make sure your computer is protected. Since it is your main way to do business and make money, you will want to make sure you don't lose it. Keep your data backed up on a separate hard drive and install virus scans and firewalls. This way, you can be fully protected.
Organize your work space in a manner that works for you. You will want to be sure to stay organized and keep everything in the proper place. This will help you save time and deal with customers easier. It also helps you complete projects in a timely manner and stay focused.
Become your own business expert. Write articles relevant to your business, and put a link in the byline or author box to your website. Include your email address if possible. Look for opportunities to speak about your expertise through online or in-person seminars. Offer to speak for organizations whose members can benefit from your products, and offer the group a share of yhour sales proceeds.
If you are looking for a way to eat potato chips while watching movies all day and still make a living, then a home based business is not the answer you are looking for. But if you implement the tips given here you can have a successful business from the comforts of your own home in no time.


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You will be required to comply with this code if you intend to operate a launch facility. A launch facility is a place specifically designed or constructed to launch space objects.
This Flight Safety Code sets out the safety requirements which you must meet to obtain a licence to operate a launch facility (a space licence).
This includes safety standards that must be achieved in relation to flight paths and in respect of the risks posed to third parties by space launches and the methodology to be used to calculate the risk.
Please consult the Contact Officer for more information and to ascertain the level of compliance that may be required.
A code of practice can be either a legal requirement or non-legal requirement. Legal codes of practice are defined as a result of legislation. Non-legal codes of practice are defined by industry regulators and bodies.
Flight Safety Code, 2nd Edition - Please consult the Contact Officer for information on this document.
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without limitation, liability in negligence, to any person for the general information
which is provided herein, or in respect of anything, including the consequences
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How do you captivate the second-largest city in the U.S., and NBA fans across the country? Make a slam dunk. By jumping over a car.
That's what the Los Angeles Clippers' Blake Griffin did on Saturday. After all the money that Kia spent on its Super Bowl ad for the new Optima sedan, its bigger coup almost certainly will turn out to be that slam dunk. The flashy new sedan was parked nearly underneath the hoop at the Staples Center in Los Angeles. Teammate Baron Davis, popping through the car's sunroof, fed the ball to Griffin as he catapulted himself across the hood and put the ball into the hoop.
A Los Angeles Times columnist, Bill Plaschke, called him the "human hood ornament" and "the man who literally gave car a jump." The feat almost certainly made every sportscast highlight reel on local evening news broadcasts over the weekend.
Chris Woodyard is an auto writer for USA TODAY who covers all aspects of motoring. He revels in the exhaust note of a Maserati and the sharp creases of a Cadillac CTS. Chris strives to live a Porsche life on a Scion budget. More about Chris


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She has cancer that's not bad enough so u have to sue her as well ? Holy cow
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I would think not. I can't imagine many people would want to work for him after this either.
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Worse Things Waiting(1973)
A collection of stories by Manly Wade Wellman
World Fantasy Best Collection
Fine cloth copy in a near-fine, very slightly edge-dulled dust wrapper, now mylar-sleeved. Remains particularly well-preserved overall; tight, bright, clean and strong.; 352 pages; A collection of the best fantasy tales from one of the genre's greatest writers. Subjects: Wellman, Manly Wade. Form/Genre: Fantasy fiction.
Used availability for Manly Wade Wellman's Worse Things Waiting
See all available used copies of this book at Abebooks UK or Abebooks US
Hardback Editions
1973 : USA Hardback
|Title: Worse Things Waiting
Author(s): Manly Wade Wellman
ISBN: 0-913796-00-X / 978-0-913796-00-9 (USA edition)
Publisher: Carcosa
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Ethics, Excess and Extinction
An exhibition exploring the reality of endangered species and humanity's responsibility to protect them
In 1998, environmental scientists proposed the concept of poaching as an environmental crime, defining any activity as illegal that contravenes the laws and regulations established to protect renewable natural resources, including the illegal harvest of wildlife with the intention of possessing, transporting, consuming, or selling it or using its body parts. They considered poaching to be one of the most serious threats to the survival of plant and animal populations. As many as 20 percent of Africa's elephants could be killed in the next 10 years if illegal poaching continues at its current rate.
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Travel Visas to Belgium
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Guide to Adrenal Health
Our adrenal system is responsible for hormones that affect our metabolism, immunity and more. Learn how to support this system and improve your overall health.
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Godzilla 201X: End Of Kaijus
Chapter: The Arrival of the King
Strong winds blew about summoning a lack of tranquility. Rising from the depths was Godzilla submerging from Tokyo bay...but where's Tokyo? Noting...
Nothing was left...did a war happened during his slumber...the only time he is at ease...or did this happened over night. Godzilla sniffed the air but no sent was found...just dust...emptiness closing in on Godzilla's heart as it's beats slowly with devastation..."WHAT HAPPENED?!" Godzilla questioned with a angry roar. Yes mankind was wiped clean off the city. Yes, Godzilla carried on his cruse on humanity but still...STILL...something wasn't right...something was wrong..
A large UFO swoops above him. Godzilla stares deeply upon this E.T with hatred. Godzilla had mixed feelings of Mankind but NO Body, No Kaiju or Alien race kills off the innocent!
The UFO was obviously carried a entity within. A spiral beam flared straight towards the UFO blasting it to sherds...
It was too easy...There's no way it's that simple, it's never easy.
The UFO was regenerating. Tendrils sport about and a roar- Oh God- A hideous roar burst out. Godzilla waited for this new opponent to rise up. The Alien had huge black pupils eyes staring at Godzilla. Razor sharp fins, Shark like fangs drooling with Alien hunch back, long arms and legs. Metallic tendrils on it's back. Tubes all over it's body, this creature was possibly an experiment of some kind. No matter, Godzilla raise his mighty voice at it. Zorg laughed at his attempt to show off his dominance. Zorg was not done morphing as it's remains of it's ship reconstructed and rewired, implanting the remains on himself. Covering his alien-nakedness, converting the ship into alien armor.
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End of Chapter one


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Onty goes to Queensland
de Rob Whennan
Acerca del libro
Another adventure with Onty the brontosaurus and his mate Mutzy the muttuburrasaurus. This time, they go on a holiday to Queensland. Anyone who has been there will recognise some or all of the places mentioned in this delightful tale of a family relaxing and enjoying both each other's company and some of the best Australia has to offer.
Características y detalles
- Categoría Niños
Vertical estándar, 20×25 cm
40 Páginas
- Fecha de publicación jul. 31, 2013
Acerca del creador
Rob Whennan was born in Murray Bridge South Australia & currently lives in the North Eastern suburbs of Adelaide. He is married with 2 children & 2 grandchildren (with more on the way hopefully). He has written various types of books, ranging from children's to horror over the years and is now, finally doing something with them. Re-typing them all from old hard copy printouts is a time consuming yet rewarding job.
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Style: 75Y0162 Color Code:
Printed woven swiss dot dress with fitted bodice and full skirt. Pintucking along the bust and smocking along the back. Elastic banding along the upper and bottom empire waist. Adjustable button straps and slip lining.
100% Cotton Machine Wash * Imported
Measurements for Size 6: Length: 37 3/8? Chest: 29?


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Two Cliks ft Jaybone & Reno Missing Me MP3
A 2013 Production!!!! From Mo' V.I.P Entertainment International.
Jay Bone Drum Cover- Kanye West- Stronger MP3
Warming up to some Yeezy.
Jaybone Bell & Restless Light "River Child" MP3
Album: Jaybone Bell & Restless Light Release Date: May 4, 2015 Video filmed in downtown Winona, MN by Brett Huus of Soundstrations Aerial footage shot by ...
Jaybone Bell & Restless Light - Guadalupe Mountains MP3
Guadalupe Mountains from Jaybone Bell & Restless Light's debut full length album set to video collage by Al & The Sharptones.
Jaybone Bell - "Tale of the Sun" - Ocooch Mountain Session MP3
An Ocooch Mountain Session featuring Jaybone Bell performing "Tale of the Sun" at Some Sum Studio in Winona, MN. Jason Ziebell: vocals/guitar Amber ...
Jaybone shuffle MP3
Jaybone dance.
Jaybone ft Ishy X & Foster - Tsvaga Bag MP3
Hustlers Anthem.
jAyBONE &jAyPEE PLAyiNG My SONG. iNAS kOOL. MP3
iNA (ME HAHA) WEA POOkAH jAyDE SiA jAyBONE AND jAyPEE AT MiCkEy DEEhS.
Jaybone MP3
Provided to YouTube by Universal Music Group International Jaybone · Milt Jackson · J.J. Johnson · Ray Brown Jackson, Johnson, Brown & Company ℗ 1983 ...
Jaybone - I Love You MP3
Jaybone brings style and grace to another level on this song. Its about a language I think we all understand......Its about love. Produced by Reno and Jaybone, ...
Destiny Destruction by DUS Jaybone MP3
Just a clip of all my favourite Crucible moments from Destiny. The song is called "Little Pills" by Devilskin. Hope you enjoy it!
JayBone-Jamming With Loops MP3
Jaybone MP3
Names a deezy.
JAYBONE & DYZZY SHOW - Ramblin' About Hulk Hogan MP3
Jaybone Bell + Restless Light MP3
Jaybone Bell and Restless Light first got together in October 2012. Prior to their first gig at Sum Some Studios in Winona, Minn., Jason performed solo under the ...
Movin On' by Jaybone Bell & Restless Light MP3
Movin' On by Jaybone Bell & Restless Light with roller skating montage. 2015 self titled album.
I'll Be Here in the Morning - Jaybone Bell MP3
Jaybone Bell covers Townes Van Zandt. What fun!!
Joey Franko ft. B6(Pushaz Ink), JayBone - Money & Bread (Remix) (Prod. Ace Santana) [New 2015] (Bes MP3
Joey Franko ft. B6(Pushaz Ink), JayBone - Money & Bread (Remix) (Prod. Ace Santana) [New 2015] (BestInTheWestRap) @JoeyFrankoLA @B6PushazinkBrow ...
Story of a felon- Jay Bone MP3
Medical Marijuana!? JayBone Tha YoungSavioso Speaks On It! MP3
Utah Artist JayBone Tha YoungSavioso Speaking On Medical Marijuana!! GET YOUR FREE DOWNLOAD OF THA SLC PUFFALATION HERE: ...
John Robeft and Jaybone MP3
Jaybone Bell and Restless Light - Cedar Valley Rally MP3
Ant Billions ft. JayBone - Anything Can Happen (Prod. DomBeatz) [New 2015] (BestInTheWestRap) MP3
[TimelessVIDZ] - Jay Bone - Puffin MP3
Track 1 from the "Go hard or Go yard" Mixtape by Jay Bone !!!! CD Coming Soon ....Keep a look out for the rest of the songs & vidz.
Jaybone Bell & Restless Light - "Lonesome" Live @ Holzinger Lodge MP3
Jaybone Bell & Restless Light live at Holzinger Lodge (4-12-14)


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Dr. Sean Esmende is a fellowship-trained orthopaedic spine surgeon specializing in treating a large variety of conditions of the cervical, thoracic and lumbar spine. These conditions include degenerative spinal disease, myelopathy, scoliosis, flatback syndrome, revision spine surgery, trauma and oncologic disorders of the spine.
Prior to coming to Connecticut, he completed his undergraduate education at Cornell University and medical school at the University of California Los Angeles. He then completed his orthopaedic residency as well as an orthopaedic trauma fellowship at Brown University/Rhode Island Hospital. He completed his spine fellowship training at the University of Pittsburgh Medical Center.
He has numerous peer-reviewed publications, written textbook chapters about spinal disorders, and has presented at national and international spine meetings.
Dr. Esmende firmly believes in the art of medicine: correlating patient symptoms along with physical exam and study results. In other words, Dr. Esmende believes in treating the patient as a whole and not just the disease. Surgery is always the last resort after trying a full compliment of conservative spinal care.


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This product has been retired from our catalog and is no longer for sale. This page is made available for those looking for datasheets and the simply curious.
Description: Replacement: None. We are no longer carrying this Sales Sheet in our catalog. This page is for reference only.
This pamphlet is a sales sheet for retailers to use for their retail displays. Have them for customers as informational take-aways. It is 3.50" by x 8.50" and printed on glossy card stock. You can use the file below to print your own. If you don't want to print your own, you can get them from us.
This pamphlet is for the SparkFun Inventors Kit.
Is the Uno on the sheet some new version? Because if you look carefully, you will see that ATMega8U2 programming header is soldered, the chip is rotated by 45° degrees and the voltage regulator is not the normal package. Also, the EEPROM (or whatever it is) is smaller than on normal UNO.
Yes, this is the newest version.
No reviews yet.


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Our first Wednesday event of the year is here! We'll be at Gresso's, 961 S High St, on the patio, Wednesday, August 24th starting at 5pm. They're hosting a great happy hour just for GETDOT…$1 GETDOT shots, a $2 beer special and wing specials all night! For those that want to stick around, Gresso's is hosting a trivia night. Great news…there's now PLENTY of parking across the street!
The raffle and live auction are also back! Check out what we have so far:
- (5) Oil Changes – Rob Howell/Noebull Automotive
- (2) $20 Average Joe's gift cards – Paul Rockwell/Average Joe's
- (2) $25 Average Joe's gift card with an Average Joe's t-shirt – Paul Rockwell/Average Joe's
- (2) $25 Gift Cards – Yabo's Tacos/Scott Boles
- Lots of t-shirts and giveaways from Gresso's
- The Skull Vodka – Tailored Management/Chip Ramsey
- Watershed Distillery Gift Basket – Bottle of 4-Peel Gin and a Bottle of Vodka autographed by the owners, passes to tour the distillery, pint glasses and t-shirt
- Mini bartending set – Tailored Management/Chip Ramsey
- A night on the town in The Gresso's Party bus for you and 20 of your friends!
- 2 tickets to the Parade of Homes Preview Party – a $190 value – The Preview party is catered by Cameron Mitchell, includes a few drink tickets and your opportunity to view the 10 homes before the general public does. – Thursday Sept. 1 from 6 til 10PM, in Powell …where the old Shamrock Golf Course was. The actual parade opens in Saturday Sept. 3rd. – Mike Whiteman
- Secret Black Box!
All proceeds from this event will go to Action for Children!
Can we count on you to make our event successful? If you're interested in donating a raffle or live auction item or becoming one of GETDOT's awesome sponsors, connect with Rob Frink, firstname.lastname@example.com, Chip Ramsey, firstname.lastname@example.com, or any of our Board Members! Any financial support or items donated are 100% tax-deductible!


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Parc Life EC
Parc Life EC is the latest new launch Executive Condo (EC) in Sembawang Crescent next to Canberra Park. Parc Life Executive Condo is developed by Frasers Centrepoint and Keong Hong. Parc Life Sembawang has 11 blocks of 15 & 16-storey towers with a total of 660 executive condo units comprising of from two, three and four bed-room units. Penthouses units is also available. The Parc Life EC site is within walking distance to the Sembawang MRT station and Bus interchange. Parc Life EC is also just a few minutes away from the conveniently Sun Plaza and the Sembawang Shopping Centre. Parc Life Executive Condo is targeted to obtain TOP in fourth quarter of year 2018.
About Parc Life EC Developer
Being one of Singapore's top developers, Fraser Centrepoint Homes(formerly known as Centrepoint Homes) has been consistently setting high standards in quality homes it has built in both Singapore and overseas markets. It has an huge and impressive portfolio of building commercial malls, executive condominiums and private residential properties with recognition such as Green Mark Awards and high Constriction Quality Assessment System. from the local authorities. With more than 13,000 homes in Singapore being build by Fraser Centrepoint Homes. It's success is because of its marketing strategy and well-timed entry in Government Land Sales P(GLS) Programmes which allows it to gain a foothold on the land sites while other developers might be holding back. It's current launches include North Park Residences, RiverTrees Residences, Twin Fountains EC, Flamingo Valley, Palm Isles, eCO, Watertown, Soleil @ Sinaran and many more. Fraser Centrepoint Homes also leaves its footprint Internationally in China, Australia, and also many parts of the world. Buyers can be assured of the quality furnishings as well as the facilities available in Parc Life Sembawang.
About Parc Life Site
(Fraser Centrepoint bags the Parc Life EC site in Sembawang Crescent)
The Housing and Development Board (HDB) has sold the residential land parcel of Parc Life EC in Sembawang Avenue, in the North Region of Singapore as part of Government Land Sales (GLS) in the 3rd quarter of 2014. This site is bounded by Sembawang Avenue, Sembawang Crescent and Gambas Crescent, and is listed under the confirmed list of sites and is allocated to be developed into Parc Life Executive Condo EC. With a plot land size of 238,849 square feet and a plot ratio of 2.8, Parclife EC Sebmawang is estimated to house 660 executive condo units. The 238,849 square feet site of Parc Life EC in Sembawang Avenue fetched four bids on Thursday. The tender fetched a top bid of $214 million from Frasers Centrepoint's FCL Tampines Court and Keong Hong Construction's KH Capital – which works out to $320 per sq ft (psf) per plot ratio (ppr) for the 238,848 sq ft plot. This narrowly edged out the second-highest bid of $211 million, or $315 psf ppr, from City Developments' Verwood Holdings and TID Residential.
Parc Life EC Location
Situated amongst developed Towns such as Sebmawang, Yishun and Woodlands, daily conveniences, grocery shopping, F&B and schools are just a stone's throw away. Parc Life Sembawang is located right between the upcoming Skypark Residences EC and Canberra Park, therefore future residents of parlife ec will get to enjoy the greenery view and vast openness of space. Parc Life EC future residents can also take a short walk down to Sun Plaza, directly just next to the Sembawang MRT Station, providing daily needs from supermarket, food court, eating joints, household products, electronics, personal care stores and pharmacy. For greater variety, future residents can also go to the nearby Sembawang Shopping Centre. New EC is also a short drive away from the Chong Pang City, a favourite neighbourhood cluster which has many retails and eating outlets. Its future residents will also be able to find many delicious and yummy local food and also fresh market produce right at Chong Pang Market & Food Centre located at Chong Pang City. Other developments in the vicinity include Canberra Residences, North Park Residences, The Nautical, One Canberra, Eight Courtyards, Yishun Emerald and Yishun Sapphire.
Facilities of Parc Life Executive Condo
Outside your home space, your life space becomes a near heaven with thoughtful and intimate facilities all around. Lounge areas covered in lush landscaping recreates life in a tropical paradise. Strategically placed spa pools will soothe you from stressed to calm. There's an aqua gym for your water aerobics. A swimming pool for your laps. And a poolside gym that adds a 'wow'to every workout. Rounding off everything is the Gourmet Pavilion for alfresco dining and… a taste of bliss. In addition, residents will also get to indulge in infinity edge pool, rejuvenating spa pools, wading pool, lawn, function room, gymnasium and green lawn/gardens within the development.
Why buy Parc Life EC?
Parc Life EC will be ideal for family living as it is near to numerous reputable local education facilities. Schools such as Sembawang Primary School, Canberra Primary School, Wellington Primary School, Endeavour Primary School, Northoaks Primary School, Greenwood Primary School, Ahmad Ibrahim Primary School, Riverside Primary School, Sembawang Secondary School, Canberra Secondary School, Ahmad Ibrahim Secondary School and Admiralty Secondary School are just within its 2 km radius. Other education amenities such as childcare centres and kindergartens can also be easily found near this new ec in sebmabang, providing parents with young children a peace of their minds while they are away at work.
Future homeowners of Parc Life Sembawang is conveniently served by the nearby Sembawang MRT station and Bus interchange. This new EC in Sebmawang is just 600 m away from Sembawang MRT Station which operates the North South Line Train services. Future residents of New EC can take a short stroll to the station. In terms of driving, For vehicle owners, Parc Life Sembawang EC is easily accessible via public transport and the upcoming North-South Expressway (NSE) will improve the overall connectivity of the road network islandwide for motorists, providing links to existing expressways such as Seletar Expressway (SLE) and Pan Island Expressway (PIE). The Sembawang new EC is approximately 24 minutes' drive to the Central Business District (CBD). When in need for a weekend shopping spree, the Orchard Shopping Belt is approximately 21 minutes' drive away. The nearby Seletar Expressway (SLE) and the Bukit Timah Expressway (BKE) allow easily driving for future residents of New EC when it comes to traveling to downtown or various parts of the island.
|Parc Life EC
|Frasers Centrepoint and Keong Hong
|Sembawang Crescent
|99 years lease from
|Site Area
|238,849 sqft
|668,779 sqft
|Plot Ratio
|Expected TOP:
|Total Units
|Total Carpark
|Executive Condominium
|Unit Type:
Amenities Near Parc Life EC
|Important Amenities Near New EC
|Approximate Distance
|Train Stations
|Sembawang (NE13)
|Bus Stop
|Opp Blk 325 (Bus: 859, 962)
Bet Blks 325/326 (Bus: 859, 962)
|Fairprice (Sun Plaza)
Giant (Sembawang Shopping Centre)
Prime (761 Yishun St 72)
Sheng Siong (Yishun Central 925)
|Shopping Malls
|Sun Plaza
Sembawang Shopping Centre
Sembawang Mart
|Education Institutions
|PCF Chong Pang Blk 317-315
PCF Canberra Blk 330-319-327-328
Edelweiss Kindergarten Sembawang Presbyterian Church
PCF Canberra Blk 413 – 405
PCF Canberra Blk 468D-468C
PCF Sembawang Blk 507D-501D-503C
Zulfa Kindergarten @ Sembawang Mart
Hope Community Kindergarten (Sembawang)
PCF Admiralty Blk 687B
PCF Canberra Blk 487-488
|Sembawang Primary School
Canberra Primary School
Wellington Primary School
Endeavour Primary School
Northoaks Primary School
Greenwood Primary School
Ahmad Ibrahim Primary School
Riverside Primary School
|Sembawang Secondary School
Canberra Secondary School
Ahmad Ibrahim Secondary School
Admiralty Secondary School
|Junior College
|Yishun Junior College
|Republic Polytechnic
|GEMS World Academy
|Food Centre
|Chong Pang Market & Food Centre
|Canberra Park
Admiral Country Club
Montreal Green
Eagle Club
HomeTeamNS @ Sembawang
To receive first hand info and floor plans and price release, please register your interest at http://parc-life-ec.com/e-application/
Other popular new property launch in 2015/2016 is Sol Acres EC, Criterion Executive Condo, Brownstone Executive Condo, Signature at Yishun, Wandervale Executive Condo, Amore EC, Lakelife EC, The Vales Executive Condo, Westwood Executive Condo and Club Gemini Residences Toa Payoh.


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February 09, 2017
Both the men's and women's cross country squads at Earlham College have been named All-Academic teams by the United States Track & Field and Cross Country Coaches Association (USTFCCCA).For a team to be considered for this distinction, it must have competed and compiled a team score at an NCAA Regional Meet. The team members also must have an accumulated minimum team grade-point average (GPA) of 3.10 on a 4.0 scale.The women's team finished with a 3.76 GPA, the third highest in all of NCAA Division III. The men's team had a 3.40 team GPA, finishing in the top half of the 173 Division III men's teams that were honored.
Earlham College, a national liberal arts college located in Richmond, Indiana, is a "College That Changes Lives." We expect our students to be fully present: to think rigorously, value directness and genuineness, and actively seek insights from differing perspectives. The values we practice at Earlham are rooted in centuries of Quaker tradition, but they also constitute the ideal toolkit for contemporary success. Earlham is one of only 40 national liberal arts colleges ranked among U.S. News and World Reports' "Great Schools at a Great Price."
Mitch Blankespoor is director of sports marketing and communications at Earlham College. He can be reached at 765-983-1795 and firstname.lastname@example.com.


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Chills Chills Chills Articles
Celebrity news that gives you the chills
Tom Cruise is not John Travolta
Rock Of Ages opens in less than a month. We're about to get a lot of the GMD. Tom Cruise as a badass rock star. Tom Cruise bare-chested, wearing eyeliner, and SINGING. Two magazine profiles have been released as advance promotion for the film. I'm sure by now that you've seen these shots of Tom fully Stacee Jaxxed in a spread for W Magazine looking really uncomfortable on the cover being groped by two models. Full Story
Isabella Cruise sees her mom ... "sometimes"
I don't know about this story. I don't know about the source. It's an Australian magazine called New Idea claiming a world exclusive one-on-one interview with Isabella Cruise, daughter of Nicole Kidman and Tom Cruise. REALLY?Fine.We'll work with it until we shouldn't work with it anymore. Just so you know in the event it turns out bogus. Full Story
The GMD's humourless child
Theo Wargo/Getty
I read an article a couple of weeks ago (thanks Anna Maria) about Newt Gingrich vs Mitt Romney that's interesting on its own merit - click to see - but here's the part that's germane to this site: For some reason, (Mitt Romney) has always reminded me of Tom Cruise, and I never could figure out exactly why that was. Full Story
Another award season without the GMD
Splash, INF
Maybe he'll be invited to present at the SAGs or the Oscars. So far though, his name has not appeared in relation to the Globes. And he certainly won't hear his name called on January 24th when the Academy announces its nominees. So we'll get little of Tom Cruise and, therefore, the presentation of Katie Holmes, on the carpets this season. Full Story
The GMD is an auror?
Gawker profiled The Delphian School today. It's K-12, at $42,000 a year per student, even though it's not yet accredited, though in the process, and was founded by Scientologists. The way the students themselves describe it, it's like, um, Hogwarts for Xenu people. Click here Full Story
Why weren't you at the Xenu party?
Saturday night in Hollywood, there was a big Scientology party attended by some of Xenu's most ardent disciples including John Travolta and wife Kelly Preston, Priscilla Presley, Juliette Lewis, Jenna Elfman, and Jason Lee, who looked hotter than he has in a long time. You can see the photos here Full Story
Robo: bland and crazy
Letterman's guest last night was Ricky Gervais. Over on The Tonight Show it was equally as boisterous as Jay Leno welcomed Katie Holmes. Hardly. That girl... Is so boring... And maybe she knows it. Maybe that's why she put on the red pants. I didn't care for the red pants. I think it's the shade of red that's the problem, especially in combination with that the colour of her crochet jumper. Full Story
Little Sci is the only explanation…
For why Katie Holmes stepped out like this in New York last night. The only. After all, she has said before that Suri picks out her clothes. God I hope so. Because if a grown woman had put all this together, and one who calls herself a "designer" at that, well f-ck me it's worse than we all thought. Full Story
The GMD on high alert
Get the popcorn ready. There's a war coming. And it looks like we all get to watch. And they're doing it Hollywood revenge styles. So, like, totally our favourite kind of catfight. Only this might bring a few more chills. Remember how Paul Haggis, Oscar-winning director, used to be a Scientologist – for over 30 years! – and he realised how f-cked they are so he broke up with Xenu in 2009 and threatened to go public with what he knows? Well… It sounds like he's going public. Full Story
You're allowed to drive for Christmas
With a chaperone. At least that looks like a chaperone. With Katie Holmes. Yesterday leaving the gym. The photo agencies are calling her companion a "friend", but you know, where Xenu is concerned, only "approved" friends are allowed. So this friend must be Church-friendly, and can you really call that a real friend? Well, small victories, I suppose. Full Story
They chose, or Xenu chose?
Curious timing... Yesterday I wrote about Granny Freeze Nicole Kidman, wondering, even though it's none of my goddamn business, as to the whereabouts of her first two children Isabella and Connor. Click here for a refresher. Judging from my inbox, it was easily yesterday's most popular post. Full Story


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Chevrolet Repair Questions? Ask a Chevy Mechanic for Answers ASAP
Ted,I'm sorry I haven't got back to you I have been real busy with my husband taking chemo and so my brother has not been able to have the car to do the work, to know if that fixes it. Will try to get it to him this weekend and get back with you.Thanks,Carol


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Do Right - Lou Holtz
Do Right, By Lou Holtz. In this first Do Right program he forcefully expresses his philosophy, along with illustrations from Notre Dame football.
Worksmarts: How to Get Along, Get Noticed, and Get Ahead
An EXCELLENT EMPLOYEE ORIENTATION PROGRAM. Promotes the idea that if employees really give their best effort daily -- rather than worrying about how to get ahead -- they will see results right away.


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- Home
- Class Schedule & Info
Class Schedule & Info
Subscribe to Our YouTube Channel To Be Notified When New Videos are Released
Class Information and Schedules
Instructors--Roland & Marsha Winbeckler
Click on topic below to quickly view the listing.
Class Information--Roland & Marsha Winbeckler Classes
Roland & Marsha have three types of classes--comprehensive 2-day courses, 1-day courses, and mini-classes (weekends OK for all).
If there's a class subject you'd like us to teach that is not listed, e-mail us.
The student fees for the classes are typically:
2-Day Courses--$800.00 per student--10 Student Minimum
- Cake Sculpture
- Airbrush
- Fondant & Modeling Chocolate Uses
- Buttercream Decorating
1-Day Courses --$400.00 per student--10 Student Minimum (Usually Two 1-Day Classes Are Taught at the Same Time To Conserve Travel Expenses, Otherwise Student Minimum Would be More)
- Cake Sculpture
- Airbrush
- Fondant & Modeling Chocolate Uses
- Buttercream Fundamentals
Mini-Classes--Price Varies by Subject, Time Required, and Instructor Supplies Needed--Combined Student Minimum for All Classes is Usually Around 65
- Airbrush Basics Demo & Class
- Basics of Cake Sculpture
- Buttercream Basics
- Cocoa Painting
- Cute Marzipan-Style Figures
- Fancy Borders w/Basic Tips
- Figure Piping--Easy Piped Figures
- Hand Molded People Figures (Like Roller Derby Figures on Ultimate Cake Off)
- Rolled Buttercream
- Wafer Paper Uses
- Writing Made Easy
Info On Sponsoring Any of Roland & Marsha's Classes
Q: How do I get the Winbeckler's to teach in my area?
A: Either you or someone you know could sponsor a class. A sponsor receives either a per-student stipend or, with enough students, a free class. A sponsor's responsibilities usually include promoting the class, taking deposits and payments from students, providing a classroom and misc. supplies, etc.
To sponsor a class(es), please read the class info above and then e-mail us to let us know which class(es) you would like to offer and a list of three date choices in your order of preference. We will try to accommodate your first choice. When choosing dates, please check the schedule below for any months that are already taken. We usually schedule just one class trip per month and do not schedule classes in July or August unless they are in conjunction with our travels to the ICES Convention.
Roland & Marsha Winbeckler's Class Schedule
October 6-8, 2017
Winbeckler Classes at Confetti Fest Cake Show--Seattle, Washington
Click this link for more class and registration information.
Designs/projects created in classes will/may vary from those shown in photos.
Easy, Fun Faces Class--Don't be Intimidated by Realistic/Comical Faces--10/6/2017 (Friday) 08:00 AM - 12:00 PM
Would you like to create realistic faces but feel intimidated and don't know where to begin? Then this is the class for you! Students will learn an easy, fun way to create realistic or exaggerated faces using modeling chocolate. The face created in class will be approximately 6" high. This is one of our students favorite classes - come join the fun! (Photo shows examples of faces created using this technique—1 Face will be created in class.) All supplies included.
Click this link for more class and registration information.
One face will be created in class. The face design made in class may not be one shown in these photos.
Writing Made Easy Class--Don't let Messy Writing Ruin Your Cakes--10/6/2017 (Friday) 01:00 PM - 3:00 PM
Learn to write on cakes like a pro. Learn the basics then do fancy script, Old English, imitation flow-in style, comical writing, etc. All these styles can be created with buttercream - no fondant cutout letters or royal icing needed. Don't let messy writing ruin your beautiful cakes!
Students should bring: Round tips #1, 2, 3, 5, & 7 (multiples of tips is helpful),plus ten (10) decorating bags (disposable plastic or parchment), and 3 couplers. Optional: 2 quarter-sheet grease-proof cake boards and boxes for students who wish to take home samples of the writing styles they create in class.
Instructors will provide: Icing, Food Colors, and Writing Surface.
Designs/projects created in class will/may vary from those shown in photos.
Click this link for more class and registration information.
Airbrush Techniques Class--Conquer Your Fear of the Airbrush--10/7/2017 (Saturday) 08:00 AM - 12:00 PM
Learn how an airbrush works, basic and advanced techniques, background possibilities with the airbrush, how to make and use stencils, and general care and maintenance of the airbrush. Also, learn ways to add shimmer/sparkle/luster dusts and how to create dimension on cakes.
Instructors will provide all supplies including airbrushes and airbrush colors for class use.
Click this link for more class and registration information.
Designs/projects created in class will/may vary from those shown in photos.
For more info and to register, see link below :
Click this link for more class and registration information.


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Obama recently said that he has friends who fly into what ever city he is in to play basketball with him. Cool. A Nike carbon footprint.
But don't worry, Al Gore has endorsed Obama. You know, the man who thinks the sky if falling because of global warming but lives in a house that burns energy like there's no tomorrow. Oh, wait, according to Gore, there is no tomorrow.