text:
Your answer is one click away!
I am creating an SSIS package that essentially attempts to find all rows in Table A that are not in Table B. The join column is an Identity column in Table A that is the clustered index and a column in Table B that is not an Identity, but is indexed. And I am doing this in batches of 10,000 rows at a time. Both table A and table B have approximately 350M rows.
I initially thought a Lookup transformation would be appropriate but I cannot use Full Cache because it attempts to load 350M rows in the cache! If I use No Cache, the process of looking up just 10,000 rows is horrendously slow (even though the lookup column in Table B is indexed).
Also, Table A and Table B are in two different databases on two different servers.
Is there another transformation that would be more appropriate for what I want to do?
Using SQL Server 2014.
You could try merge join component in the data flow task.
Actually when deal with large amount of rows, you could try some other ways to improve the performance, such as importing both tables into a staging database for sql join, as set operation is usually faster then row by row process.


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I have been on this wiki for a long time, i have done my fair share of contribution and recently i have seen a lot of vandalism, here is some examples of recent vandalism
"*Glenn is one of two characters who has a china face,the other being Vince ." Which was on Glenns VG Page
"Cayla was turned by a Super Zombie named Musiah Robinson" Which was on Cayla Ridleys Page
And a lot more on her page-
Hair = Fire Crotch
and her description -
On October 8th, 1993 Cayla Christine Ridley was found roaming the woods around Eatonton ,GA leading a pack of Red Wolves. While her actual birthdate is unknown, the age of the chile was estimated to be around 3 years. Initially, the young ferral demon toddler had no verbal skills, or blatter control, and responded only to the smell of propane. Communication was limmited to a series of bird like screaches and complicated patterns of arm swinging.
Upon her discovery, the American Foundation for Human Developement (AFHD) labeled their new find "Specimen Ging", shipped her to the big city of Atlanta, and administered a series of tests and therapies to the child to measure her mental capasities. While early signs showed little promise, persisting doctors began to notice small improvements in attention span and visual recognition of basic shapes through years of electroshock therapy.
By the age of 20, Specimen Ging had developed the mental faculties of an average kindergartener and the AFHD was losing interest in the study as quickly as they were losing revinue. Soon after, the once famed failed science experiment was thrown out on the street to fend for herself once more. However this time around, she was living in a concrete jungle. The only learned trait that she had absorbed through repeated conditioning was to moan incessently for avocados and vodka until her needs were met.
On Flames Page
and currently on flames page
and on ThatGuyWhoLikesTWD Page
===You know when your playing chess and you want to get around the other persons pawns to take out all the real threats, Poops are meant to be in the backround, thats what I think zombie movies are all about, its not about the zombies at all actually, its all about the survivors trying to survive in a world that has no hope." - Zach===
And bear in mind this was in the past HOUR. Who thinks we should ban anon's from editing? And if they want to edit they should make an account

subset: commoncrawl


text:
They learned the meaning later that morning, when they bumped into a neighbor from their hometown of Kaneohe, which is on Oahu, one of Hawaii's eight islands. Suddenly, Las Vegas seemed awfully familiar.
"It looks like we're back home," said a surprised Joel Kekauoha, 37, making his first trip to Las Vegas. "You see all your friends up in Las Vegas, not at home."
More people from Hawaii moved to Nevada from 1995 to 2000 than the combined populations of the islands of Lanai and Molokai, and the equivalent of half the Hawaiian population travels every year to and from Las Vegas.
Coming to Las Vegas "can be like a reunion," said Paul Kosasa, who found a ready market in the desert for three of Waikiki's ubiquitous ABC stores. The Las Vegas branches — part hotel-style gift shop, part convenience store, part drug store — remain the only three of the 67-store chain in the mainland United States.
Indeed, when 200 transplanted Hawaiians gather every two years from their adopted homes in California, Washington, Oregon and Minnesota, they rendezvous in downtown Las Vegas.
"Hawaii people are natural gamblers," said Ted Kamada, a reunion organizer who graduated from high school in Maui in 1950. "Las Vegas is a natural thing for them."
Steven Lum, a Las Vegas real estate businessman, estimates there were a few hundred Hawaiians among the 600,000 people living in southern Nevada when he arrived in 1986.
Since then, about 25,000 people have swapped Hawaiian driver's licenses for Nevada licenses, according to Nevada Department of Motor Vehicles records. Today, Nevada has 2.3 million residents.
From 1995 to 2000, some 12,079 people moved from Hawaii to Nevada, according to the U.S. Census, outstripping the combined number of 10,597 residents on Lanai and Molokai.
"In Vegas, you have a higher standard of living," Lum said. "In Hawaii, the quality of life is better. It depends on what the person wants."
Melissa Nahooikaika's family chose opportunity. When the 18-year-old and her parents were looking to move before she entered high school, they put Kona and the Big Island behind them and put down roots in southern Nevada. Her dad found work building houses in one of the fastest-growing areas in the nation.
"The price of living was really high there," Nahooikaika recalled as she stepped from a Jeep Cherokee with "Hawaiian By Blood" stenciled on the rear window.
Everyone in Hawaii knows someone who moved to Las Vegas, said tourist Melodi Kekauoha, 36. She said that while visiting the city she and her husband planned to see friends who made the leap across the Pacific.
To meet increased demand, Hawaiian Airlines plans to add a redeye flight in April 2005 and Aloha Airlines launched a direct daily flight this year between Vegas' McCarran International Airport and Honolulu. Air travel from the islands is up nearly 27% from January to August this year.
McCarran, which tallies both commercial and charter passengers, reported 690,772 traveled to and from Hawaii in 2003. For such a small state — Hawaii has a population of only 1.2 million — it is in the top 12 for visitation to Las Vegas.
"The demand for Las Vegas travel in Hawaii is huge," said Keoni Wagner, marketing vice president for Hawaiian Airlines in Honolulu.
For many Hawaiians, their first trip from Honolulu to Las Vegas was on a cheap 1970s charter flight and a $9.90 room-and-meal package arranged by Boyd Gaming Corp., owner of several downtown hotels.
Today, about 80% of Boyd's customers come from Hawaii, said John Repetti, a company vice president. Company charters bring 10,000 travelers a month from Hawaii.
"Customers who come in, I used to deal to their father or grandfather," Repetti said. "People come to our properties because they've been coming for generations."


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The ignitor squad of Tarot deals next to games vie using this platform of uniquely odd cards. These extent from the notably dense to those a four-year-old can romp. "Zarcana," a complex team game broad battles, voyages, life, death, love, loss, and much can be contend indefinitely - or until one artist in a conquering situation decides to claim the activity fattened. Another unfit appeals to the little set because of its overmuch simpler rules. Each recitalist is dealt a card and some sign is on the card, the entertainer must decree a visual aspect. This can be to a certain extent zany and fun for little brood. Imagine the bring joy to once a artist is dealt The Fool!
There are as more Tarot games as here are Tarot aficionados, and a sudden expression through the Internet can isolate much Tarot games than a individual could humanly strive to drama in one time period. But this is the joy of Tarot - and of the Internet!
As with games, Tarot platform designs run the orbit as asymptomatic. After all, these card game have been in a circle for hundreds of years, and each society has contributed its own book. Sometimes even individuals have come up away beside their own versions of the game, and several of these have turn reasonably undemanding. The Waite-Smith Tarot interpretation (also familiar as "Rider-Waite") of the cards, created during the archeozoic bit of the 20th century, has go belike the peak popular ornamentation used today. Along beside Waite-Smith, quite a few of the known and still-used Tarot versions list the Visconti-Sforza, the Marseille, and the Minchiate. These latter decks repeatedly feature highly wrought graphics minus a acute business of unseeable allegory and, depending on of her own tastes, can sometimes be construed as masterpieces of Renaissance logo.
Many teachers of Tarot urge victimisation the Waite-Smith publication until the initiate has turn up to date beside all the internal representation meanings and representations. At this point, the beginner can after continue to prime from the some Tarot designs open the platform that appeals to him or her individually. It is not outlandish for even a long-time practitioner of Tarot to go on using the Waite-Smith revision because, as one Tarot student says, "It is simple, forthright, and appeals to the pragmatic on the side of my nature."
Tarot paper decks can be purchased some places both online and at brick-and-mortar retailers. Because the reproductive structure of "devil worship" has moved out the Tarot in lots parts, sometimes one can find a platform in as dull a stand as Wal-Mart. Other areas may lonesome transfer decks in hard to find fortune-teller equip shops and opposite "underground" markets.
Tarot serves to amuse as very well as to indefinite quantity insight, much as it did hundreds of old age ago. So drop in your own deck and switch on today to gather the rewards of study Tarot. You may indefinite quantity more than than you ever imagined!


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|Epha4, 2900005C20Rik, AI385584, Cek8, Hek8, Sek, Sek1, Tyro1
|A DNA sequence encoding the human EPHA4 (NP_031962.2) extracellular domain (Met 1-Thr 547) was expressed, with a polyhistidine tag at the C-terminus.
|Human Cells
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 96 % as determined by SDS-PAGE
|Measured by its ability to bind biotinylated Human EphrinA5-his (cat: 10192-H08H) in functional ELISA.
|< 1.0 EU per μg of the protein as determined by the LAL method
|Samples are stable for up to twelve months from date of receipt at -70℃
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EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.
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text:
What's in North Korea's Military Arsenal?
Player utilities
This story is based on a radio interview. Listen to the full interview.
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This story is based on a radio interview. Listen to the full interview.
Marco Werman: I'm Marco Werman, this is The World. If you want to know what North Korea's leaders are up to, don't expect a press conference with statements of fact. You'll likely learn more by gauging the tone of state TV broadcasts from Pyongyang. North Korean state TV programs complete with menacing sounding soundtracks carry the latest pronouncements from the regime. Lately, the broadcasts have been full of threats and bluster. Today, the bluster was in the form of a poem entitled Final War with the US Imperialists. The reading is totally over the top. So is the music that sounds like a score for a scene in The Godfather, when Michael Corleone kills his nephew in the restaurant. It kind of makes you chuckle rather than shake in your boots, but US officials are not laughing. Today, Defense Secretary Chuck Hagel warned that North Korea is skating very close to a dangerous line with all its anti-American threats. So what can North Korea actually do? Does the country's military have what it takes to engage in a final war with US imperialists, as the poem goes. I asked Joel Wit to give us a reality check on Pyongyang's missile capabilities. Wit is a former US negotiator with North Korea.
Joel Wit: They're working on a range of different missiles from ones that might eventually reach the United States to ones that can hit targets in the region. In terms of what they definitely have now I would say they probably can reach Guam with the missile that everyone's been talking about them testing. And they certainly can reach Japan and South Korea.
Werman: So are these missiles armed? I mean if they reach Guam, what then happens?
Wit: Well, look, don't forget. We're talking about them testing a missile. There's no one who thinks they're gonna mount a nuclear warhead on top of the missile and firing at Guam right now. There's no reason for them to do that, and there's no sign that they're preparing for that kind of general conflict. So you know, the missile they're gonna fire up now is a medium, what's called a medium range missile and they're just going to be testing it to see how it works or doesn't work.
Werman: And do they have in their capability and in their military capability, warheads that can go on these missiles?
Wit: Well, you know, the experts disagree on this. I know some very reputable experts who think they can put a nuclear warhead on top of the Musudan or similar missiles, not on top of ICBMs. And there are other experts who say no they can't. So you know, we're really not sure about that, but we shouldn't forget that they've been working for over 20 years on developing nuclear warheads for missiles, so if they haven't developed them by now, they've been doing a pretty bad job of it.
Werman: So that's the background on the missiles in North Korea. Nukes are another story. What kind of nuclear weapons does North Korea certainly have and how soon could they be put on a missile?
Wit: Well, what we know now is that North Korea probably has around eight nuclear weapons based on plutonium that they produced over the past decade. And that North Korea seems to be working on another way of producing material for nuclear weapons and that's enriching uranium. But we don't know whether that part of their program is operational yet, and as I said earlier, there's a division between experts on whether the North can put any of these weapons on top of even the medium or shorter range systems, but I tend to believe given the length of time they've been working on it that they can probably do that.
Werman: Joel Wit, we've seen of course, this amping up of rhetoric before from North Korea, from Kim and his family, but his family and grandfather usually left room for an exit strategy, a pressure valve of sorts to get out of confrontation mode. How much room has Kim, though, given himself?
Wit: Oh, I don't think it's a problem for him to move forward with an exit strategy. You know, they'll fire the missile off maybe. I'm not sure they will, but that's what all the reports say. They'll have their April 15 celebration, which is normal for every year. Then we have a US-South Korean exercise going on, which isn't going to end until the end of April, so we can expect more tensions the rest of the month, but at the end of the month I think things will calm down and he can claim victory that he's kind of defended his country, and then he'll move on.
Werman: Joel Wit, former US negotiator with North Korea, thank you.
Wit: Thanks a lot.


text:
Original Dune
This article or section refers to elements from Original Dune.
The Imperial Council was the ruling body of the Atreides Empire after Paul Atreides took the Golden Lion Throne from House Corrino.
Its members included:
- Paul Atreides, the 82nd Padishah Emperor of the Known Universe.
- Irulan Corrino, his wife, the Princess-Consort.
- Chani Kynes, his concubine.
- Alia Atreides, his sister, a Reverend Mother.
- Korba, his Quizarte priest.


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The Roc is an island formed by a single enormous mountain jutting out of the ocean.[1]
The peak is in the clouds, the lower slopes are covered in dense forest. It is located between lands of Kish and the Isle of Volcanoes. It gets its name from the bird which still resides there.
See AlsoEdit
- ↑ Seas of Blood - ???; Titan - The Fighting Fantasy World - p.??/??


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Pet Sitter in San Diego
My name is Jennifer. I enjoy being able to make any pets day by allowing them to be out in the open even if it is for a certain amount of time a day. If you are looking for someone to fill that errand in your schedule, I am the person for you. I have a dog myself and know where to go to assure them a fun time out.
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Beautifully done wedding church pew decorations will make the church look not just pretty but magical. The pews can be decorated in a very elegant manner and this can be done for sensible price which is what couples are searching for today.
One of the most commonly used inexpensive items that are used as pew decorations is tulle. You can find tulle for wedding church pew decorations at almost every store and it's not at all difficult to use it for decoration.
An advantage of using tulle is that it lasts long and can be safely travelled over long distances easily - if you want your church pew decor done before time. Today many weddings are theme based, so you can use the tulle in line with the wedding theme for the pew decoration.
Another option would be to use ribbon or lace. Wide ribbon/lace can be draped and run along the top of the pew. You could use narrow widths create a swag effect between the pews and use a decorative item to hide the peak of the swag drape.
Ribbon or lace can also be made into bows and rosettes that can be hung from the sides of the pews.
All of the above can be used on their own or teamed with flowers and other decorative features to create spectacular looks.
Some more wonderful pew church decoration ideas are to use flowers or greenery. These can be displayed in inexpensive containters such as decorative cones, small buckets or simply tied together with decorative ribbon.
Other inexpensive wedding church pew decorations which can be used are unique hurricane lamps, unlighted tiki torches and even just a single candle which has been tied to a lovely floral bouquet. A floral designer or a good florist would be able to guide and help you in developing a special and lovely floral display which can be placed at the pews ends.
Stick to your decided budget and it would be astounding to see how much you can do for wedding church pew decorations. Imagination has no limits - go ahead and experiment with ideas!
Once the ceremony is completed leave the venue and form a receiving line then greet and thank your guests for coming as they exit the ceremony venue.
You can read more tips and ideas by clicking here.
If you are planning a winter wedding and still want to wear that strapless dress, see if can hire out a cape from your local gown shop. A cape will keep you warm and should help avoid those goose bumps showing up in your wedding photos.
If you want to look slimmer in your wedding photos stand slightly side on, rather than standing square in front of the camera.
There are many wonderful ways to capture your day, use our wedding photography shot list as a guide to help you create a list of lasting memories to include in your wedding album.


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Welcome to the stunning location of Ashaig House – sit back and enjoy the views!
croft /krôft/
"A small rented farm, esp. one in Scotland, comprising a plot of arable land attached to a house, sharing pasturage with other such farms…"
- Sandy Beach
- 5 Minutes beyond Skye Bridge
- Spectacular sea views
- En-suite rooms
- Two Peaceful Lounges
- Piano, books & games
Ashaig House is a refurbished crofthouse set on seven acres of croftland sweeping down to the sea. The unique and tranquil setting of the house represents an ideal central access point for touring Skye and neighbouring Lochalsh.
This modernised cottage presents a home-from-home amid glorious Hebridean scenery. Next to a sandy beach in the peaceful Skye crofting township of Breakish, the location is exceptional, boasting uninterrupted views and sunsets across the nearby islands of Pabbay, Raasay, Scalpay & Crowlin. This is complemented by stunning panoramic views of the Red Cuillins, and outlooks toward the Cuillin Ridge, Torridon and Old Man Of Storr.
There is parking right outside the house, and free WiFi Internet access indoors. The croftland in front of the house is fine for ball games, barbeques and kite-flying.
The accommodation is self-rated as a high 3-star level. The East section of Ashaig House is not made available for let and is not occupied. Ashaig House is entirely non-smoking.
Walkers, Adventurers, Kids, Geologists, Romantics – your Hebridean bolt-hole awaits…
The comfortable bedrooms comprise one Double Room Ensuite (upstairs), one Family Room Ensuite (upstairs) and a Twin Room with adjacent private bathroom (downstairs). The upstairs bedrooms are traditional attic rooms with extensive sea & mountain views, while the ground floor bedroom has partial views towards the Cuillin Hills and Broadford Bay.
One of the original crofthouse bedrooms, the Double Room Ensuite is a compact room located in the attic space of the original cottage, with its own WC and shower.
Our Family Room Ensuite is also located in the attic space of the original cottage and has a standard double plus a single bed. It has a small ensuite WC and shower. The room is typically booked by two individuals, but may also be suitable for a family.
Our comfortable Twin Room is located on the ground floor, with its own private WC, bath & shower.
Ideal for families/groups of 4-7, but extendable to 10 people: A further 4th bedroom and study-bedroom can be booked (at additional cost) for large parties of 8-10 persons – this must be requested at time of booking. These 2 rooms are not made available to groups of 7 or less. Please contact us for an accurate quote.
Please feel free to email email@example.com or call UK mobile telephone 07801 748 840.


text:
Style 2391; ©1969; Child's and Girl's Cape: The lined cape has panelled front with hand openings in the seams. View A has collar and front button fastening. View B and C have edge-to-edge front. View B has stand collar and chain-link fasteners. View C is collarless with sating bias binding and bow trim.
Links to reviews/blog postsEdit
Add a photo to the gallery by clicking the "modify" button below.
[insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!]


text:
A case with all of the extras.
Designed for professionals, this fine hardshell case features a soft wine velvet interior, string tube, suspension padding, hygrometer, 4 English bow clips and a luxurious blanket. The exterior has leather accents, TSA lock, built in rain-jacket, large front pocket and sturdy handle with hook 'n loop leatherette closure, and includes a shoulder strap.
- Comfort Features
- Quick-lock closure
- Organizer built in exterior pocket
- Backpackable with optional backpack strap (model #BPSTRAP)
- Includes padded shoulder strap and ID tag
- Subway handle strap with contoured rubber grip
- Interior Features
- 4 ribbon-backed bow holders
- Lined with soft velvet
- Hygrometer
- String tube
- Suspension System
- Detachable Blanket
- Large accessory compartment
- Exterior Features
- Lightweight shock absorbing wood frame
- Waterproof 1680D Ballistic Nylon Exterior
- Leather Accents
- Large Front Pocket
- Backside sheet music pocket
- Built-in rain jacket
- TSA Lock
- TSA Lock Features
- 1. Using a pen, push down reset button (located on top)
- 2. Dial your desired combination
- 3. Slide open lock to set new combination
- PLEASE NOTE: A key is not included with TSA lock cases; keyhole for TSA personnel use only
Order today and get everything you want in a case: quality, protection and value.
Double Professional 4/4 Violin Pro Pac Case Specifications:
- Exterior Dimensions: 31" x 11.5" x 5"
Weight: 7.72 lbs.
REVIEW SNAPSHOT®by PowerReviews
Reviewed by 1 customer
- Easy To Use
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- Needs More Inside Storage
Best Uses
- Backup
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- Practicing
Comments about Protec Double Professional 4/4 Violin Pro Pac Case:
Overall the case is nice. Not too heavy, yet sturdy enough. Fit for the violins is excellent. Inside storage is very small. It would be nice to have enough room to get a shoulder rest inside. On the other hand the ouside pockets are nice. My ipad fits in the one on the back nicely. The full length pocket on the top holds a fair amount of stuff. As to durability, time will tell. It appears to be well made. The fabric is heavy enough, we will see about the bow spinners and zippers. It is nice being able to have both fiddles without have 2 cases.


text:
PLYMOUTH, N.H. – Plymouth State College is gearing up for a new academic year. Approximately 3,500 students are expected to return to campus over the Labor Day weekend, and PSC Campus Police will be implementing a new traffic plan to better direct College related traffic on move-in day, and help alleviate congestion in downtown Plymouth.
"Due to current construction projects on campus, gridlock in the downtown area in previous years and concern over roadway access for emergency vehicles, we, along with other local law enforcement, will coordinate the flow of traffic on move-in day," says Campus Police Chief John E. Clark.
On Sunday, September 2, electronic signage will be positioned on Interstate 93 just south of Exit 25 directing all PSC traffic to Exit 26. After exiting, drivers will be directed south on Main St., then west on to Merrill St., which provides access to all on and off campus housing. High St. will be open to eastbound traffic only due to construction at Prospect Hall.
Campus police, along with local and state police, will be stationed throughout the area to assist drivers. State police will be present off Exit 25 to redirect PSC traffic north on I-93 as needed. There will also be officers stationed on Main St. at the intersections of Merrill St. and Holderness Rd./Rte. 175A.
Additionally, members of PSC's athletic teams and Greek life will be on hand to help students unload vehicles and move in to on-campus housing. Their assistance will further aid police in maintaining the flow of traffic through the community.
"Our goal is to facilitate a smooth and efficient move-in experience for all students, keep local traffic flowing and ensure the roadways are accessible to emergency vehicles," says Clark.
A copy of the map indicating the move-in day traffic plan is available at http://www.plymouth.edu/psc/pscnews/move_in_map.pdf


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My Man eLiquid by One Hit Wonder 180ml
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Bulk Adventure Time Tattoos 2400 ct
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This article was interesting. though im somewhat disappointed it turned into a little bit of a "blame game" of the US/UK vs developing countries. The issue is that these there is no sustainability built into the business model of the VFX industry. Most companies are living project to project and even though this is a real issue in the service industry I think there needs to be some way to get some fairness and profitability back into the industry so that artists can focus on creating amazing art instead of how they are going to pay their bills and feed their family after the next job is done. I think Peter Jackson is in a unique position to comment on this as he cofounded weta and directs so he knows that even though the studios need the VFX guys to make amazing graphics and sell some movies there isnt any vested interest in one VFX company over the other so instead of saying "hey guys were going to work with you guys on the next film" and the VFX company having a relationship with the film studio to make great film the relationship is more like a prostitute. If something happens to one VFX house the studio feels like they are expendable and can find another house capable of doing the work. Even if it isnt really that way they can use that to push the price down.
This is the issue with the service industry in general. How do you find a methodology of billing that will allow the offices to stay open in the inbetween time from projects reduce the exploitation thats going on that seems to be killing a lot of amazing offices?
Not sure the answer to this but I think this is the real question of the article.


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Amish Garden Furniture Collection
This Yellow Pine Garden Bridge is Amish made from untreated American grown knot-free Southern Yellow Pine Wood. This 4' x 12' bridge is the perfect connector to your peaceful oasis. It creates a beautiful path to your garden, across a small pond or to any other area of your yard. The decorative spindles add an extra special touch to dress up any outdoor living space. The slight arch in this bridge allows it to blend in with a quiet and subtle environment or for a stylish flair add a little color in the finish.
The Yellow Pine bridge is shown unfinished, however it can be customized with any of the nine available Natural- Kote, soybased, eco-friendly stains. Choose any of the finishes in the blue 'Customize it' button to the right of the picture or to be sure you will be satisfied, request a stain sample from us.
© Copyrights 2003 - 2017 DutchCrafters All rights reserved.


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Mt. Eaton Collection
Complete the look with our Plum Creek Dining Set. The Plum Creek Double Pedestal Table and chairs have a country style that is perfect for the dining room or kitchen nook. This pedestal table comes standard with a boat shaped top and chamfer table edge. it is shown in oak wood with our OCS 102 stain but has nine wood options and many more finishes. Call to receive wood and finish samples to help you determine your options for this solid wood dining table. You can also order wood and finish samples online.
© Copyrights 2003 - 2017 DutchCrafters All rights reserved.


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Rough, tumble and even stumble featured during spectacular racing over 24hours at Moonee Valley, Lankan Rupee being a major contributor, but Adelaide, benefitting from a trouble-free passage, stole the show on Saturday.
Rarely, if ever, have there been group1 finishes with so many in the photo, as in Friday night's Manikato Stakes, described as one of the best, most exciting races seen, despite the hard-luck stories.
The field of seven ended within two lengths of Lankan Rupee in the major sprint following mayhem in the early stages.
Sure, Irish stallion Adelaide deservedly gets the accolades for the Cox Plate triumph and was the best horse in the contest under the circumstances, but he did have a trouble-free run around the outside.
Certainly Fawkner, beaten a short neck, was given every chance by Nick Hall after stumbling badly atthe start.
"Well, it didn't do him any good," Hall said. Hall was able to get him balanced and into a perfect position.
Hall then had to make the first move towards the lead on a hot pace which he conceded "put him in a spot to be beaten".
Champion rider Damien Oliver, who handled Happy Trails, rated the race as "the most exciting finish I've ridden in since Super Impose [1992]" but "suited horses running on from behind".
But not Criterion, of the David Payne stable, under the circumstances. "It's a rough game, this," Payne said after Criterion struck trouble before ending up seventh. Nash Rawiller [Silent Achiever] was later suspended over the incident.
Tom Berry [Foreteller], too, figured the fifth placegetter should have finished closer.
Of course, it comes as jockeys are being criticised for showing no quarter. The Cox Plate has always been a battleground.
Earlier on Saturday's program Chad Schofield came down at the turn on Albonetti in the Moonee Valley Cup, took a count and got back on his feet. The race was won by Prince Of Penzance, Michelle Payne riding bold.
"When we got the run today it was tight coming around the corner," Michelle Payne said after thesuccess.
Still, before Adelaide came onto the scene, the major turf topic was Lankan Rupee and the Manikato protests. Official race caller Greg Miles commented: "It took forever."
Despite the world's best video technology the hearing, and decision, probably set a record for the time taken to get the result.
"We had to tick all the boxes," Racing Victoria chief steward Terry Bailey said.
Oliver lodged a protest on behalf of Angelic Light, the second placegetter, while Tim Clark, who handled Famous Seamus, also objected against Lankan Rupee in a Manikato riddled with early interference.
Trying to sort out the fracas,e stewards questioned Damian Browne (Buffering) and Tegan Harrison (Temple Of Boom) as well as Newitt and the offended jockeys.
Questions arose regarding witnesses being called but, while not a regular occurrence, it is not unique to get a just outcome. Finally stewards deduced the interference to Angelic Light was a combination of Temple Of Boom initially shifting in before being taken further by Lankan Rupee.
However Newitt straightened his mount and the decision to dismiss was based on interference to Angelic Light not being fully attributed to the winner. Regarding Famous Seamus, the stewards felt the gelding "was being tightened prior Lankan Rupee having an influence".
Newitt was issued with neither asevere reprimand, which did not sit well with many, nor the merit for the victory.


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Для получения актуальных цен по гостинице Bw Hotel Globus City, пожалуйста, укажите интересующие вас даты и выполните поиск.
Located in Forli, Bw Hotel Globus City is minutes from Puntadiferro Shopping Centre and close to Sanctuary of Santa Maria Delle Grazie. This 4.5-star hotel is within close proximity of Parco di Via Dragoni and Abbey of San Mercuriale.
Этажей: 1
|Описание номеров
|Make yourself at home in one of the 98 air-conditioned rooms featuring minibars. Complimentary wireless Internet access keeps you connected, and digital programming is available for your entertainment. Bathrooms have bathtubs or showers and hair dryers. Conveniences include phones, as well as laptop-compatible safes and desks.
Rec, Spa, Premium Amenities
Take time to pamper yourself with a visit to the full-service spa. You're sure to appreciate the recreational amenities, including a health club, a spa tub, and a sauna.
Satisfy your appetite at the hotel's restaurant, which serves lunch and dinner, or stay in and take advantage of room service (during limited hours). Quench your thirst with your favorite drink at a bar/lounge.
|Обратите внимание
|Featured amenities include a business center, a computer station, and express check-in. Free self parking is available onsite.


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Lindsay Lohan Is Telling People She FaceTimed Oprah On Christmas
Almost nothing in life is certain. For example, who would've guessed Charlie Sheen would get HIV? But if there's one truth with which we can all rest peacefully in our graves, it's that there's no goddamn fucking way Oprah spent Christmas morning FaceTiming Lindsay Lohan. For people like Oprah, Christmas morning is spent sleeping in while your staff incinerates the bodies you and your guests flayed to death in ritual sacrifice to an owl god the night before. Unless Stedman asked Santa for a video of a cokehead rambling about how some Greek guys she spent the summer blowing on a yacht are financing her big comeback. Guy goes to one party at Tara Reid's house and all of a sudden he can't get off unless a former child star is crying near a billionaire's penis.
THE SUPERFICIAL | About • Facebook • Twitter
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What is it? Auditory Flashcards!
By Fuze Inc.
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What is it? is a fun way to expand your child's vocabulary in an engagingly visual and auditory environment. The What is it? series is designed around the concept that presenting visual stimulus along side audio not only improves a child's ability to memorize the vocabulary being presented but also becomes a source of entertainment, further increasing their desire to learn.
What is it? is a series of auditory flash cards that not only present the terms in clear and concise audio, but also adds fun with jingles and sound affects. Watch your child's face light up as they hear the siren of a police car, the roar of a lion, and the timbre of the violin! Your child's English vocabulary is sure to broaden their understanding and command of the English language in a matter of days!
Over 180 vocabulary words in full color detail with accompanying voice and audio that not only asks the questions, but answers them upon the students request with the push of a button!
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- Ice
- 1 1/2 ounces Old Tom gin, preferably Ransom
- 3/4 ounce sweet vermouth
- 1 teaspoon maraschino liqueur
- Dash of Angostura bitters
- Dash of orange bitters
- 1 lemon twist, for garnish
Fill a pint glass with ice. Add all of the remaining ingredients except the garnish and stir well. Strain into a chilled coupe and garnish with the lemon twist.


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And yes, every sewing project asks its sacrifices, I really every time prick myself with a needle...
Flower bag
On Friday I was with a friend of mine in a big fabric store and 'cause I got some really nice fabrics I replaced my crochet hook by my sewing machine and sewed this bag. (Will soon add it to my shop) I already sewed 4 like that, but this time I made a lining with an inner bag. It took me a whole day to finish. The pattern is my own design of which I'm quite proud of :)
Eingestellt von wideeyedtree um 1:12 PM
5 comments:
- [s.n.] said...
oohh, hübsch! :)
hofftl bekomm ich auch mal so aufwendige taschen hin! <3
liebe grüße,
- 13.2.11
- Lini said...
voll schön, da bleibt blutvergiessen ned aus ;-)
- 13.2.11
- Bianca said...
the blood shot is actually kind of cute, if blood can be cute. :)
- 14.2.11
- studio meez said...
Lovely bag! I like the fabric you used.
- 14.2.11
- Wiebke kritzelt said...
Dornröschen ;-P
- 3.11.11
Subscribe to: Post Comments (Atom)


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
Recently got a DUI in town, Still in the process of going to court with a civilian attorney. My command (USN) is sending me to Captains mast, others in my command have gotten DUI's and never went to captains mast or received any NJP. Since my case is still in the process on the civilian side and, I have not been found guilty of anything ,is it basically an admission of guilt from being punished at NJP and can they use results from that in civilian courts? Is there any way I can stop my command from sending me to NJP? Are my fifth amendment rights being violated?
As far as the civilian courts they cannot use any of the proceedings from your NJP or anything that your command does against you on the civilian side.
As far as what you're Command does I would speak with a JAG officer and have them give you counsel regarding that. It's my understanding that they can proceed forward with the NJP however they still need to provide evidence to use against you during the NJP process.
William Daley, Ret. USMC
I will be happy to speak with you at no cost with a FREE phone consultation if you have a San Diego or California matter. Please call my office at 619-238-1905 or visit my website at www.lawofficeofwilliamdaley.comAsk a similar question
speak to a JAG officer. Probably not to many jag officers answering questions on AVVO.
Legal disclaimer: This message does not constitute legal advice and is for informational purposes only. This message does not establish an attorney-client relationship, which can only be established once a retainer agreement has been fully executed between you and this firm.Ask a similar question
Why are you not directing this question to your "civilian attorney" and/or naval representative(s)? They are the ones who should be in the best position to answer your questions as it relates to your specific case, particularly since the case(s) have already gone to court/NJP.
Law Offices of David Shapiro 3555 4th Avenue San Diego, CA 92103 (619) 295-3555Ask a similar question
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|Home > Fidelix
♂ Fidelix
What does Fidelix mean?
Fidelix [fi-delix] as a name for boys is a Latin name, and Fidelix means "faithful". Fidelix is an alternate form of Fidel (Latin): from the Latin names Fidelis.
ASSOCIATED WITH faithful
ALTERNATIVE FORMS VIA FIDEL Fadelio, Fedele, Fedelio, Fidal, Fidalio, Fidele, Fidelio, Fidelis, Fidello, FideloCREATIVE FORMS
(male) Codelix, Fidedi, ..
(female) Fideli, Fidenia, .. MIDDLE NAME PAIRINGS
Fidelix Azaiah (F.A.), ..
How popular is Fidelix?
Fidelix is an uncommon first name for males. Fidelix is a rare last name too for both adults and children. (2000 U.S. CENSUS)
Which version is better?with its source form and related boy names. Fidel (#1643 LAST YEAR) is the only popular varying form of Fidelix (OUTSIDE TOP 2000). Adoption of this form of Fidelix reached its apex 14 years ago (#832) and is somewhat lower today. (TOP 2000 NAMES, 2015)
Similar NamesSuggested similar names are Feidhlim, Felic, Felim, Felix▲, Felyx, Fenix, Field, Filip, Fillip, Findlie (see Finlay), Finlie, Finnlie and Videlio. These names tend to be more frequently used than Fidelix.See names in meaning and etymology.


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|Publication number
|Publication type
|Application number
|Publication date
|Oct 28, 1986
|Filing date
|Oct 7, 1985
|Priority date
|Oct 7, 1985
|Fee status
|Publication number
|Robert W. Bull, Robert M. Soltysiak, Paul D. Minnick
|Original Assignee
|Neogen Corporation
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (10), Non-Patent Citations (8), Referenced by (13), Classifications (12), Legal Events (6)
|External Links: USPTO, USPTO Assignment, Espacenet
(1) Field of the Invention
The present invention relates to an equine vaccination method wherein the vaccine is potentiated by a leukokine or mixed leukokines administered with the vaccine. In particular, the present invention relates to the potentiation of an equine influenza vaccine with mixed equine leukokines.
(2) Prior Art
The prior art relating to interferon is extensive. U.S. Pat. No. 3,699,222 (1972) to Isaacs and Lindenmann describes the original research with interferon as an antiviral agent. U.S. Pat. No. 4,503,035 (1985) to Pestka and Rubinstein describes purified interferons and in particular the use of leukocytes and a virus (Newcastle Disease virus) for inducing the interferons. Example 7 of this patent particularly describes the use of this procedure to produce equine interferbn.
The term "interferon" is generally used by the prior art to refer to induced proteins of leucocyte and of fibroblast origins which interfere with viral replication. The terms "leukokine" or "leucokine" have been used in the recent literature to characterize proteins including interferons of leukocyte origins and the term leukokine is used herein.
The presence of interferons in the blood stream with virus vaccines is known to increase or enhance the immune antibody response. U.S. Pat. No. 3,906,092 to Hilleman, Tytell and Woodhour (1975) describes the use of interferon inducing polynucleotides as adjuvants to non-replicating (killed virus) vaccines to enhance antibody formation. The administration of mixed live Newcastle disease virus vaccine and interferon to provide an enhanced protection for chickens is described as prior art in this patent, but there is no description of the administration of interferon and a non-replicating virus vaccine. It is not believed that the use of interferons with equine vaccines has been described by the prior art.
Vaccination with commercially available inactivated equine influenza virus vaccine alone (consisting of killed virus of tissue culture origin) produces a variable humoral immune antibody response in equines. This variation is in the magnitude of the antibody response, the time course in which the equine responds to the vaccine, and the duration of the antibody response. These variations have been recognized and accepted as due to biological variation of the species' immune system. Where there is an urgent need for immunity, the equine is repeatedly vaccinated with influenza vaccine.
New and more potent vaccines which augment the equine's immune response to influenza or other viral vaccine vaccination and thereby greatly increase both the likelihood and extent of a protected period with less frequent vaccination, are needed. Further, vaccines are needed where there is a less variable response to the vaccination.
It is therefore an object of the present invention to provide improved vaccine compositions containing an admixture of an equine replicating or non-replicating virus vaccine and of a leukokine as an adjuvant, wherein there is an increased antibody response from use of the composition in the equines. Further it is an object of the present invention to provide a method for administering the vaccine with the leukokine which provides the improved response. It is particularly an object of the present invention to provide an improved method and vaccine compositions for providing immunity to equine influenza. These and other objects will become increasingly apparent by reference to the following description and the drawings.
FIG. 1 is a graph of serum antibody response in equines to separate and concurrent coadministered injections of bivalent equine influenza vaccine (Haver-Lockhart Labs, Shawnee, Kansas) (combined Miami and Prague strains) (1) Equi-1 and Equi-2 with a level of equine leukokines of 200,000 International Antiviral Units, (IU) or (2) with no leukokines. The equines had a high initial serum antibody titer of 1:60 or higher to A/Equi-2/Miami/1/63 which appears to indicate some level of immunity most likely as a result of prior exposure to influenza virus.
FIG. 2 is a graph showing the same separate and concurrent coadministration as in the graph FIG. 1, i.e. (1) (200,000 IU) or (2) no leukokines, except that the equines had a low initial antibody titer of 1:20 or less.
FIG. 3 is a graph showing the same separate and concurrent coadministration as in the graph FIG. 1, except that (1) the leukokines were coadministered with the vaccine at a low level (20,000 IU) or (2) no leukokines were administered with the vaccine. The equines initially had a high level of serum antibody of 1:60 or higher.
FIG. 4 is a graph showing the same coadministration as in the graph FIG. 2, except that the leukokines were coadministered (1) at a low level (20,000 IU) or (2) no leukokine was administered. The equines initially had a low level of serum antibody of 1:20 or less.
The present invention relates to an improvement in a method for providing an immune response to an equine disease with a viral or viral subunit or other antigen vaccine which produces a blood serum antibody response to the vaccine in the equine which comprises: administering an equine leukokine with the vaccine to thereby provide an enhanced immune response in the equine. The method particularly relates to the treatment of equine influenza.
Further the present invention relates to a vaccine composition for an equine which comprises in admixture: a viral or viral subunit or other antigen vaccine which produces a blood serum antibody response to the vaccine in the equine to provide an immune response to a viral disease; and an equine leukokine as an adjuvant to the vaccine, wherein the leukokine is present in an amount which increases the blood serum antibody response to the vaccine in the equine. The leukokine generally substantially increases the initial antibody response in the equines.
The leukokines induced by the virus without purification is mixed with other leukokines of differing molecular size and no attempt is made to purify the leukokines because of the expense; however, this can be done in the manner of U.S. Pat. No. 4,503,035. It is expected that the purified leukokines administered with the vaccine would individually produce more or less antibody response in the equine.
The most practical method of producing the leukokines is by virus induction of equine leukocytes in vitro. The preferred virus produces Newcastle disease in chickens. Other viruses which can be used, for instance, are Bluetongue virus, Sindbis virus and Sendai virus. It is generally impractical to isolate the leukokines in vivo. Recombinant genetic techniques can be used to produce the leukokines in bacteria.
The viral, viral subunit or other antigen vaccines are very well known to those skilled in the art. It is especially unexpected that the leukokines would enhance the serum antibody of non-replicating vaccines, such as the equine influenza virus vaccines. The virus are killed by formalin in producing the equine influenza vaccine used in the preferred compositions and method of the present invention.
Examples of vaccines where the antibody response in equines can be enhanced are those intended for prophylaxis of viral arteritis; equine encephalomyelitis (Eastern, Western Encephalitis, equine sleeping sickness); equine rhinopneumonitis (herpesvirus type 1); equine abortion (herpesvirus type 3); African horse sickness; and foal gastroenteritis (equine rotavirus).
The vaccine compositions can be administered to the equines in any convenient manner. The most common means is by injection, although nasal sprays and other techniques for blood stream absorption vaccines can be used. The vaccines can be separately administered or administered together. The vaccine dosages are usually administered in dosages of 1 ml, usually 0.1 to 5 ml intramuscularly.
The vaccine compositions used are well known to those skilled in the art. U.S. Pat. No. 4,466,957 to Hjorth et al, U.S. Pat. Nos. 4,009,258 and 3,518,347, for instance, describe influenza vaccines.
The vaccines can include other conventional adjuvants. These include complete or incomplete Freund's, aluminum hydroxide, peanut oil, by way of example. U.S. Pat. Nos. 3,790,655 and 3,919,411 to Carlson et al describe adjuvants used in the vaccines used in the specific examples.
Preferably the dosage of the leukokine used with the vaccine is between about 20,000 and 1,000,000 International Units. The assay for the leukokines used in the present invention uses a human interferon preparation developed by the National Institute of Health as a reference standard as discussed herein.
In particular, equine mixed leukokines (EML), when adinistered separately intramuscularly along with a formalin-inactivated combined equine (influenza) virus vaccine, cause an increase of humoral immune antibody response to the vaccine antigen in equines. The EML are produced by induction of isolated equine leukocytes with Newcastle Disease Virus in vitro. The EML were extracted and partially purified by diafiltration and ultrafiltration but the induced leukokines are not isolated. The increase in antibodies reactive with the vaccine antigens occurs more rapidly and is of greater magnitude with the leukokines than the antibody response from the vaccine alone. This translates to a greater assurance of protection against equine (influenza) virus, with the resultant economic and practical benefits of realizing that protection from fewer vaccinations.
1. Collection and Preparation of Equine Leukocytes.
a. Equine peripheral blood leukocytes were collected by a process of leukophoresis in which up to 10 L of peripheral blood from a single animal were subjected to processing. The peripheral blood leukocytes were retained and the red blood cells and plasma returned to the animal.
b. The volume of the peripheral blood leukocytes obtained was assessed in an appropriate vessel and the cells were diluted to a volume three times the starting volume with cold Tris-HCl buffered ammonium chloride (140 mM NH4 Cl, 6mM Tris-HCl), pH 7.4, to initiate the elimination of any remaining red blood cells.
c. After five minutes incubation at room temperature, with frequent agitation, the cell suspension was centrifuged at 500�g for 15 minutes at 4 degrees Centigrade.
d. The cell pellet was resuspended in a minimal amount of Hank's Balanced Salt Solution without Ca2+ and Mg2+ (HBSS), pH 7.4, and centrifuged at 500�g for 15 minutes at 4� C.
e. Following a subsequent wash with HBSS, pH 7.4, the cells were resuspended in a known volume of HBSS.
f. A portion of the cell suspension was diluted 1:20 with HBSS and the total number of cells estimated by hemocytometer count.
g. Cells in the original suspension were pelleted by centrifugation at 500�g for 15 minutes at 4 degrees C. and resuspended in nutrient medium to result in a final concentration of 1�10 (7) cells /ml.
2. Preparation of Newcastle Disease Virus (NDV) for Induction, and Determination of Hemagglutination Titer.
a. NDV (strain Bl, Hitchner; ATTC VR-108) was inoculated into the allantoic cavity of 10 day embryonated eggs, and allantoic fluid containing virus was harvested after a further 72 hour incubation. The allantoic fluids were pooled so as to result in several lots.
b. Freshly drawn chicken blood was mixed 1:1 with cold Alsever's solution (0.42% NaCl, 0.8% trisodium citrate, 2.05% glucose, 0.055% citric acid, pH 7.4) and centrifuged at 500�g for 15 minutes.
c. Serum and Alsever's solution were decanted and discarded.
d. Cells were resuspended in Alsever's solution, mixed thoroughly, and centrifuged at 500�g for 15 minutes.
e. Resuspension of cells, centrifugation, and decantation were repeated.
f. From the packed cell volume, a 0.5% suspension of the chick red blood cells (CRBC) was made in PBS.
g. To each of twelve (12) consecutive wells of a 96 well microtiter plate (v-shaped wells) was added 0.1 ml PBS.
h. 0.1 ml of allantoic fluid from a single lot was added to the first well.
i. Serial 2-fold dilutions were made by transferring 0.1 ml of each consecutive dilution out through well 12.
j. 0.1 ml of the 0.5% CRBC suspension was added to each well.
k. The above procedure was followed for every lot of NDV-containing allantoic fluid in the current pool.
l. 0.1 ml of the 0.5% CRBC suspension was added to 3 wells containing only 0.1 ml PBS (negative control wells).
m. Hemagglutination was assessed when CRBC's in the control wells form "buttons" in the bottom of the wells (the "buttons" ran when the plate was slightly tilted). The titer was equal to the reciprocal of the highest dilution which interferes with "button" formation of the control type, expressed in hemagglutinating units/ml (HAU/ml).
n. Individual lots from the current pool of allantoic fluid which showed the highest HAU/ml were pooled for induction.
o. The induction pool was dispensed such that each aliquot contains enough NDV for 3 to 5 liters of nutrient induction medium, based on a final 15 HAU/ml. Aliquots were stored at -70 degrees Centigrade until use.
3. Preparation of Nutrient Medium for Leukocyte Culture.
a. The nutrient medium for maintenance of the isolated equine peripheral blood leukocytes in which the cells were induced to produce EML, was similar to that described by N. N. Iscove and F. Melchers. (J. Exp. Med 147:923-933, 1978), with the modifications specified below:
1. Iscove's Modification of Dulbecco's Modified Eagle's Medium (IDMEM; Gibco, Grand Island, N.Y.) was rehydrated according to package directions.
2. Addition was made of alpha-thioglycerol (Sigma, St. Louis, MO.) to a concentration of 75 mcM, in nutrient medium.
3. An emulsion of soybean lecithin (type II-S; Sigma) was made in 100 ml of Dulbecco's Modified Eagle's Medium (DMEM) also containing 10 mg/ml fatty acid-free bovine serum albumin (FAF-BSA; Sigma), pH 5.0, by trituration and homogenization of 750 mg of lecithin and including it in the final 10 ml of DMEM--BSA. This mixture was filtered through a sterile 0.45 micron membrane (Millipore) before addition to the nutrient medium to achieve the final concentrations of lecithin (75 mcg/ml) and BSA (100 mcg/ml).
4. Fetuin (Spiro method; Gibco) was added to the nutrient medium to achieve final concentration of 150 mcg/ml.
5. Transferrin (Gibco), 4 mg/ml was 1/3 saturated by the addition of 296 nmol Fe(NO3)3, passed through a 0.2 micron filter (Millipore, Bedford, MA), and added to the nutrient medium to a final transferrin concentration of 1 mcg/ml.
4. Induction of Equine Leukocytes for Production, and Recovery of the EML.
a. The washed equine leukocytes were suspended in the complete nutrient medium at a final concentration of 1�107 cells/ml in the induction vessel (BioCul Type 20 Cell Culture System; Queue Systems, Parkersburg W.V.). To this cell suspension were added EML, previously induced, purified and concentrated by this procedure, to a final concentration of 50 antiviral units/ml of nutrient medium.
b. The contents of the induction vessel were gently stirred in a constant temperature waterbath (37� C.) for 5 hours. During this and the subsequent incubation, the pH was monitored by an internal probe and adjusted to pH 7.4 by automatic addition of acid or base.
c. After 5 hours of incubation, allantoic fluid grown Newcastle Disease Virus (NDV) was added to a final concentration of 15 HAU/ml. The HAU of the NDV pool had previously been assayed by standard methods (Sec. 2 above).
d. Incubation was continued for 18 to 20 hours at 37� and pH 7.4 with gentle stirring to maintain the cells in suspension.
e. The contents of the induction vessel were transferred aseptically to sterile centrifuge bottles and centrifuged at 1500�g 30 minutes at 4� C. The cell pellet was discarded.
f. A sample of the supernatant was taken for centrifugation at 100,000�g for 1 hour before assay for antiviral activity by the plaque reduction method described hereinafter. The balance was held at -20� C. until processing.
5. Diafiltration, Ultrafiltration and Concentration of EML.
a. Supernatant collected from the induction vessel was diafiltered using an Amicon DC 10L ultrafiltration unit fitted with a 100,000 MW cut-off hollow fiber cartridge (H5P100-43; Amicon Corp., Danvers, MA). Prior to diafiltration the cartridge was cleaned and equilibrated by sequentially running through the unit:
1. Five liters of 0.2 M NaOH for 20 minutes,
2. Five liters of distilled H2 O for 2 rinses of 20 minutes each,
3. Two liters of phosphate buffered saline (0.8% NaCl, 0.02% KCl, 0.12% Na2 HPO4, 0.02% KH2 PO4 ; PBS), pH 9.0, containing 0.5% fraction V albumin, for 20 minutes,
4. Two liters of 5 mM sodium phosphate buffer, pH 7.4, for 20 minutes.
After loading the supernatant, diafiltration was initiated with 6 to 7 volumes of 5 mM sodium phosphate buffer, pH 7.4, which resulted in reduction of the reservoir volume to approximately 500 ml. Samples were removed for assay of antiviral activity and to determine absence or presence of live virus.
b. Following decontamination of the ultrafiltration unit by a 20 minute rinse with 5 L of 0.2 N NaOH, it was next fitted with a hollow-fiber cartridge with a 10,000 MW cut-off limit (H10P10-20) and steps 1) through 4) of 4.a. above were repeated.
c. The diafiltrate was ultrafiltered and concentrated against the 10,000 MW hollow fiber cartridge to approximately 500 ml.
d. The remaining crude EML product was removed from the reservoir, filter sterilized by use of a 0.2 micron filter apparatus (Millipore), distributed to sterile vials, and lyophilized.
e. Contents of random vials were taken for assessment of sterility, antiviral activity, protein content, and other biochemical characterizations.
6. Plaque Assay for Antiviral Activity of EML.
a. Virus plaque reduction assay was performed as described by Langford, et al. (Methods in Enzymology, V. 78, Academic Press, New York, N.Y., p. 339, 1981), utilizing monolayers of equine dermal fibroblasts (E. Derm, ATCC-CCL57) challenged with Vesicular Stomatitis virus.
b. In this assay, utilizing WISH human amniotic cells, NIH Reference Human Interferon Alpha (HuIFN-alpha[Leukocyte/Sendai]Ga23-902-530), having an assigned potency of 4.08 log 10 International Units (IU)/ml, demonstrates 4.59 log10IU/ml.
1. The EML, produced as described above, were reconstituted from the lyophilized state with sterile distilled water so as to result in an injection volume of 1 to 5 ml containing 20,000 to 200,000 units of antiviral activity for intramuscular administration to the animals.
2. At the time of vaccination with the commercially available formalin-inactivated equine influenza virus vaccine, the EML were separately co-administered by intramuscular injection. Subcutaneous injection could also have been used.
3. Humoral antibody responses of animals after injection with the vaccine alone or with EML injection were assessed by standard hemagglutination inhibition (HAI) tests as modified from Hierholzer, J.C., Suggs, M. T, and Hall, E. C. (Appl. Microbiol. 18:824-833, 1969) as adapted for the microtiter method, using as antigen allantoic fluid-grown influenza viruses of the same prototype strains as used in the equine vaccine (currently these are A/equi-2/Miami/1/63 and A/equi-2/Prague/1/56).
a. Equine sera for assay were pretreated and diluted as follows:
1. To 0.5 ml serum was added 2.0 ml borate-buffered saline (0.7% NaCl, 0.3% H3 BO3, 0.01% NaOH; BBS), pH 9.0, and 2.5 ml of a 25% suspension of acid-washed kaolin (Sigma) in BBS, pH 9.0.
2. The solution was shaken vigorously at 5 minute intervals for 20 minutes at 24 degrees Centigrade.
3. The solution was centrifuged at 800�g for 30 minutes.
4. The supernatant was decanted to separate tubes. This represents a 1:10 dilution of original serum.
5. The diluted serum was inactivated at 56 degrees Centigrade for 30 minutes.
b. The hemagglutination inhibition (HAI) test was run as follows using 96 well microtiter trays:
1. 25 mcl PBS was added to wells 2 through 12 of rows designated for serum dilutions.
2. 50 mcl PBS was added to 3 wells for cell controls, and to 6 others for virus "back-titration".
3. 50 mcl of each of the starting serum dilutions (1:10) was added to the first wells of each of two rows in which serum dilutions will be made.
4. Serial 2-fold dilutions of the sera by transfer of 25 mcl were made to each well out through well 12 of each row.
5. 25 mcl of PBS was added to all wells of every second row (these were serum control wells).
6. The appropriate allantoic fluid-grown influenza virus in PBS were diluted such that 0.1 ml contains 16 HAU of activity (Sec. 8.A.2., determination of HAU).
7. 25 mcl (4 HAU) of the virus dilution were added to all wells of the remaining rows which contain serum dilutions (these are the serum-antigen wells).
8. 50 mcl of the virus dilution were added to the first of the wells for antigen "back-titration".
9. Further serial 2-fold dilutions of the virus dilution were made by transfer of 50 mcl to each well through well 6.
10. The plates were shaken carefully and incubate at 24 degrees Centigrade for 30 minutes.
11. 50 mcl of a 0.5% suspension of CRBC's (Sec. 8.A.2.b-f) were added to all wells.
12. The plates were shaken carefully, and incubation continued.
13. The plates were read for HAI when the cell control wells showed compact "buttons". Cell control "buttons" ran when the plate was tilted, as should any HAI-positive wells (those where hemagglutination had been inhibited by antibody). HAI-negative wells show an even distribution of CRBC over the bottom of the wells. The HAI titer of the serum was expressed as the reciprocal of the serum dilution (starting dilution is 1:10) in the last well to show a "button" (no hemagglutination).
14. Serum control wells should not show hemagglutination (auto-agglutination); any auto-agglutination may mask low serum antibody titers.
15. Virus "back-titration" should indicate the presence of 4 HAU/25 mcl. Typically, the first 3 wells will show hemagglutination, the last 3 wells will "button".
At the time of vaccination of an equine with killed equine influenza virus vaccine, 20,000 to 1,000,000 units of EML was also administered intramuscularly, preferably 200,000 to 400,000 units. FIGS. 1 to 4 show the results of the vaccination. The results are quite pronounced where the equines had higher levels of the antiflu antibody initially. Administration of higher levels of leukokines also produced improved results. As shown in the Figures the equines were revaccinated at day 31 to 34. The influenza vaccine was in a dosage of 1 ml.
FIGS. 1 to 4 show vaccinations as follows:
HAI LO Group: serum titer 1:20 or less to A/Equi-2/Miami/1/63 at start of study.
Vaccinate+EML (200,000 IU antiviral activity)
Vaccinate+EML (20,000 IU antiviral activity)
Vaccinate-no EML (control group)
HAI HI Group: serum titer 1:60 or higher to A/Equi-2/Miami/1/63 at start of study)
Vaccinate+EML (200,000 IU antiviral activity)
Vaccinate+EML (20,000 IU antiviral activity)
Vaccinate-no EML (control group)
Three equines were used per group. The EML equines receive EML on two occasions (when vaccinated). Blood is drawn every second day for about 70 days. All equines received the second vaccination with/without EML on approximately day 33. Serum anti-flu titer in FIGS. 1 to 4 refers to the reciprocal of the serum dilution resulting in inhibition of erythrocyte agglutination.
As can be seen from the foregoing specific description the EML of the present invention are very effective in increasing the serum antibody titer for equine influenza vaccine. Similar effects can be expected with other vaccines since the results should not depend upon a particular vaccine antigen.
|Cited Patent
|Filing date
|Publication date
|May 28, 1965
|Jun 30, 1970
|Inst Microbiologie Et D Hygien
|Vaccine for equine influenza
|Aug 5, 1968
|Oct 17, 1972
|Nat Res Dev
|Production of viral interfering substances
|Jan 31, 1972
|Feb 5, 1974
|Haver Lockhart Labor Inc
|Injectable adjuvant,method of preparing same and compositions including such adjuvant
|Jan 20, 1971
|Feb 19, 1974
|Richardson Merrell Inc
|Equine strangles vaccine and method of preparing and using the same
|Nov 26, 1971
|Sep 16, 1975
|Merck & Co Inc
|Stimulation of antibody response
|Feb 4, 1974
|Nov 11, 1975
|Bayvet Corp
|Injectable adjuvant and compositions including such adjuvant
|Apr 3, 1973
|Jul 20, 1976
|Baugh Clarence L
|Interferon production
|Aug 5, 1974
|Feb 22, 1977
|The Mount Sinai School Of Medicine Of The City University Of New York
|Influenza vaccine containing a recombinant, antigenically hybridized virus and method of using the same
|Feb 25, 1982
|Aug 21, 1984
|American Home Products Corporation
|Vaccine adjuvants
|Feb 14, 1983
|Mar 5, 1985
|Hoffmann-La Roche Inc.
|Protein purification process and product
|Antibiotiki, 12(7), 602 604 (1967), Litvinov.
|Antibiotiki, 12(7), 602-604 (1967), Litvinov.
|Hierholzer, J.C. et al., Appl. Microbiol. 18:824 833 (1969).
|Hierholzer, J.C. et al., Appl. Microbiol. 18:824-833 (1969).
|Iscove, N. N., et al., J. Exp Med. 147: 923 933 (1978).
|Iscove, N. N., et al., J. Exp Med. 147: 923-933 (1978).
|Langford et al., Methods in Enzymology, vol. 78, Academic Press, N.Y., N.Y., p. 339, (1981).
|Litvinov, Chem. Abstracts, 67, 7536,80070u, 1967.
|Citing Patent
|Filing date
|Publication date
|Sep 10, 1986
|Aug 25, 1987
|Neogen Corporation
|Method for administering vaccines containing equine leukokines and compositions therefor
|Oct 23, 1986
|Mar 22, 1988
|Peter Stephan Center, Ltd.
|Polyvalent equine immune serum composition and method for treating rheumatoid arthritis
|Aug 31, 1998
|May 9, 2000
|Chiron Corporation
|Human IL-2 as a vaccine adjuvant
|Mar 24, 1994
|Jul 4, 2000
|3M Innovative Properties Company
|1H-imidazo[4,5-C]quinolin-4-amines as vaccine adjuvants
|Apr 16, 2004
|Jul 17, 2007
|Board Of Regents For Oklahoma State University
|DNA vaccine expressing HA1 of equine-2 influenza virus
|Nov 23, 2005
|Aug 13, 2013
|La Prairie Group Ag
|Tissue culture media used as a component of cosmetics
|Mar 1, 2005
|Aug 27, 2013
|La Prairie Group Ag
|Cosmetic or dermatological preparation comprising a nutrient medium phase
|Apr 16, 2004
|Feb 10, 2005
|Alexander Lai
|DNA vaccine expressing HA1 of equine-2 influenza virus
|Oct 19, 2004
|Nov 10, 2005
|Beiersdorf Ag
|Cosmetic or dermatological preparation comprising a nutrient medium phase
|Mar 1, 2005
|Dec 29, 2005
|Beiersdorf Ag
|Cosmetic or dermatological preparation comprising a nutrient medium phase
|Nov 23, 2005
|Aug 17, 2006
|Juvena (International) Ag,
|Tissue culture media used as a component of cosmetics
|Mar 31, 1988
|Oct 5, 1988
|Mallinckrodt Group Inc.
|Synergistic immunostimulating composition and method
|Mar 31, 1988
|May 30, 1990
|International Minerals And Chemical Corporation
|Synergistic immunostimulating composition and method
|U.S. Classification
|424/85.4, 424/278.1, 424/209.1, 424/85.7, 424/214.1
|International Classification
|Cooperative Classification
|European Classification
|Oct 7, 1985
Owner name: NEOGEN CORPORATION 241 EAST SAGINAW, SUITE 517 EAS
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:BULL, ROBERT W.;SOLTYSIAK, ROBERT M.;REEL/FRAME:004467/0954
Effective date: 19851001
Owner name: NEOGEN CORPORATION 241 EAST SAGINAW, SUITE 517, EA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:MINNICK, PAUL D.;REEL/FRAME:004467/0956
Effective date: 19851004
|Apr 14, 1987
|Certificate of correction
|Jan 12, 1990
|Fee payment
Year of fee payment: 4
|Jun 7, 1994
|Maintenance fee reminder mailed
|Oct 30, 1994
|Lapse for failure to pay maintenance fees
|Jan 10, 1995
|Expired due to failure to pay maintenance fee
Effective date: 19941102


text:
Teach Basic Computer Skills - all ages, all abilities
Cause Areas
WhenIt's flexible! We'll work with your schedule.
Where415 N Edgeworth StreetSuite 175Greensboro, NC 27401
HandyCapable Network helps bridge the technology gap in our community by distributing low and no-cost computers to families and individuals without computers in their home. As an extension of this part of our mission, we would like to offer computer skills classes to our computer recipients and anyone else in the community with a desire to learn.
Classes offered will include courses in basic computer skills such as Microsoft Office products, basic web page design, how to use social media, and anything else that has to do with operating a computer.
We would like to open classes to youth and adults of various ages - from school age to senior adults, both English speaking and non-English speaking.
If you are interested in helping promote computer literacy in our community, please contact us today!
- Basic Computer Skills
- IT Consulting
- Microsoft Office Suite
- Computer Science
- Security / Network Administration
- General Education
Good Match For
Requirements & Commitment
- Must be at least 16


text:
PLAQUEMINE — Iberville Parish Sheriff Brent Allain announced in February that he would forgo running for a fourth term in office in favor of spending more time with his family.
When Allain made that announcement, it set off a flurry of speculation about who would succeed him.
Parish President Mitch Ourso, a former deputy whose late father, Jessel Ourso, served as sheriff from 1964 to 1968 and again from 1972 until 1978, was one of the most talked-about possible successors to Allain.
While the parish president initially said he was "seriously thinking" about making a run for sheriff, he ultimately released a statement the day after Allain's announcement saying he would stay out of the race.
In the weeks and months afterward, speculation around the parish was focused on Sheriff's Office personnel and police chiefs throughout the parish who might enter the race.
The picture of who exactly would seek the parish's top law enforcement job didn't become clear until last month when the qualifying period for potential candidates ended.
When it was over, several candidates including current and former law enforcement officers, investigators and businessmen had all thrown their hats in the ring.
On Oct. 22, Iberville Parish voters will have the task of selecting from a field of nine, the parish's next sheriff.
The nine candidates are:
e_SBlt Kevin "Butchie" Ambeau Sr., a Democrat from Carville.
e_SBlt Harold Brooks Jr., a Democrat from White Castle.
e_SBlt Michael "Coupella" Garvin, a Republican from Grosse Tete.
e_SBlt Kelvin Q. Goins, No Party from Plaquemine.
e_SBlt Lionel Johnson, a Democrat from Plaquemine.
e_SBlt Wyatt Neely, a Democrat from Plaquemine.
e_SBlt Aubrey St. Angelo Jr., a Democrat from Plaquemine.
e_SBlt Brett M. Stassi, a Democrat from Plaquemine.
• Glynn Stassia, a Democrat from Plaquemine.


text:
Worship--On Campus and Off
Ohio Northern is affiliated with The United Methodist Church, and has been since 1899. We recognize our connection with the denomination in a lot of ways, but the most important of those is Chapel Worship, which takes place on Thursdays at 11 a.m. This time of worship and fellowship includes singing, scriptures and a message, prayer, and most importantly--food! Lunch is served each week for all students, faculty, and staff who attend worship.
On Sundays, and on other days of the week, there are a wide variety of places where you can connect with God and a congregation of local, loving friends:
Click below to download our Chapel Worship Speakers brochure:


text:
Clinton Truman Duffy
Clinton Truman Duffy (1898 – 1982) was the warden of San Quentin State Prison between 1940 and 1952.[1] He was a prominent opponent of capital punishment.[1]
His father was a guard at San Quentin, he was raised on the prison grounds, and his wife's father was also a San Quentin guard.[1]
The 1954 film Duffy of San Quentin tells his story as a warden.[2][3] His accomplishments during his tenure as warden include:[1][4]
- Elimination of corporal punishment
- Improvement of food services
- Establishment of vocational training
- Founding of an Alcoholics Anonymous program
- Desegregation of the dining hall
- Creation of the first prisoner-developed radio programs inside a prison
- Inauguration of a prison newspaper
He was known to walk unarmed among the prisoners and to chat with them.[1][4] Although he "supervised 90 executions during his tenure as warden," he opposed capital punishment.[1]
After he left San Quentin, he worked for the state's parole board; in addition, he wrote books and gave lectures about capital punishment.[1] He died in Walnut Creek, California at the age of 84.[1]
- Duffy, Clinton T., and Dean Southern Jennings. The San Quentin story. Garden City, NY: Doubleday, 1950.
- Duffy, Clinton T. 88 men and 2 women. Garden City, NY: Doubleday, 1962.
- Duffy, Clinton T., and Al Hirshberg. Sex and crime. Garden City, NY: Doubleday, 1965.
- Duffy, Clinton T., and Eva Irene Linkletter. From heroin to San Quentin. Morro Bay, CA: Java Books, 1977.
- ^ a b c d e f g h Blake, Gene (1982-10-14). "Famed warden Duffy of San Quentin dead at 84". Los Angeles Times.
- ^ Duffy of San Quentin (1954) on Internet Movie Database
- ^ Crowther, Bosley. "Duffy of San Quentin (1954)". New York Times. Retrieved 2009-01-11.
- ^ a b "Mister San Quentin". Time Magazine. 1952-01-07. Retrieved 2009-01-12.
External links[edit]
|This biography article about law enforcement in the United States is a stub. You can help Wikipedia by expanding it.


text:
The UntouchablesThe Lily Dallas Story - Season 2, Episode 21 - 16 Mar. 1961 - Lily Dallas is a highly intelligent ex-con who has a history of committing bank robberies. This time she and her husband George 'Blackie' Dallas, who is known for his deft use of a Tommy gun, kidnap a well known and very rich businessman Thomas B. Randall and demand $300,000 ransom.
Back to the The Untouchables Library
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back to Uncle Earl's Classic TV Channel


text:
Changes in planning rules that would make it easier for businesses to expand should be welcomed by SMEs, says Jason Stockwood.
It's been a tough few months for poor Vince Cable. Yesterday must have been particularly difficult as he watched the completion of the BSkyB deal, from which he was unceremoniously removed at the end of last year.
Last night, though, Uncle Vince gave a speech at the Mansion House, in which he set out some potentially significant changes to the planning system in this country.
Mr Cable said that the government wants to flip the planning process on its head, and build a system in which the "standard answer is 'yes', not 'no'."
Broadly, the intention is for the changes to make it easier for businesses to get the planning permission they need to expand or to start new projects. The government would also be able to give special priority to certain big infrastructure developments.
George Osborne apparently intends to put the new planning process at the heart of a new package that he hopes will jolt the economy back into growth – without actually requiring any up-front government spending. The package will be presented in the Growth Review, which is due to be announced alongside the Budget.
As things currently stand, the planning process is pretty dysfunctional. The extraordinary amount of red tape through which businesses must battle is unsustainable, and there is little doubt that this is preventing expansion. Any moves that could reduce that red tape burden are to be welcomed.
Similarly, we are fully behind any proposals that could make it easier for SMEs to grow. A change to the planning system, if properly executed, would make it simpler for businesses to develop the facilities they need. At the same time this could be great news for the construction industry, which is crying out for government support.
Of course, this is also good news for the country as a whole. Government is constantly extolling the abilities of the SME community to boost growth, and repeating the fact that small businesses will be the leaders of the economic recovery. At Simply Business we have long been calling for some concrete action to back up these words, and a properly considered, efficiently executed change to the planning system would certainly be a welcome step.
One note of caution, though. It is of course necessary for the planning process to take into consideration the wishes of communities. There is some concern that the proposals could see those wishes side-tracked, in favour of the demands of businesses. A system like this would be bad for communities, and bad for business.
We would like to see a system in which businesses and residents can work together to find solutions that work for everyone. We hope that the government's Growth Review will bring us one step closer to this reality.
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