text:
open/close all folders
Anime and Manga
- Berserk
- A pregnant (not that this was known at the time) Casca is raped by the demon Femto after his creation. This drives Casca insane and corrupts the fetus into a demonic-like grotesque.
- Emperor Ganishka's demon soldiers are created by dumping heavily pregnant women into an enormous artificial womb that radiates the woman full of demonic energy. The now-demonic fetus rips its way out of the uterus and joins the others.
- Guts' biological mother was executed/murder while she was heavily pregnant with him, and he was delivered more or less as a coffin birth.
- In Vinland Saga, Arneis (a slave) is secretly pregnant with her master Ketil's child. When her former husband comes to take her back - slaying several guards of the farm in the process - and she tries to escape with him, Ketil does not take it well. Just before her punishment, she reveals to him she is pregnant with his child; his response is to suspect that the child isn't his, and beat her half to death. And not only half, as she dies shortly after.
- The pilot of Cowboy Bebop had a heavily pregnant woman on the run with a man wanted for drug smuggling, with the police in hot pursuit. When she is finally gunned down, the belly turns out to be a huge bundle of Red Eye spray canisters.
- In the EC Comics story "Squeeze Play" (Shock Suspenstories #13), the word "pregnant" is never used (it was the 1950s, after all), but the story revolves around a man who kills his girlfriend after she informs him that he HAS to marry her.
- In Sky Doll, the main character, a love doll named Noa has a very graphic hallucination when she is pregnant and her womb rips out. But it's all in a dream... or is it?
- In Watchmen The Comedian shoots a pregnant woman during the Vietnam War. Doubles as Offing the Offspring, as the child was his.
- A 1991 issue of Wolverine featured an Eldritch Abomination that was targeting three pregnant women on a cruise ship, because one of them was carrying The Chosen One but the demon couldn't tell which one. The demon killed two of the women before Wolverine could save them, but Logan did manage to save the right one in the end.
- In the 1991 mini-series The Punisher: P.O.V., an eight-months-pregnant woman is attacked and bitten by a mutated villain. She survives the attack, but later dies during the delivery because the bite had mutated her unborn child.
- In The Punisher MAX X-Mas Special, a gangster's pregnant wife is the target of hitmen sent by a rival gangster out to wipe out her husband's entire family. The Punisher saves the pregnant target from the killers and helps her deliver the baby...at which point he executes her himself for her own crimes, now that the innocent child is out of danger.
- In The Metabarons, the Metabaron Aghora has the brain of a male and female body of his twin sister since he was born dead and had his brain implanted into the body of his twin sister who was brain-dead at birth to become a S/he. S/he goes into a Despair Event Horizon after of the death of her pet spider-wolf and gets over this funk by getting her/himself pregnant. This is when enemies of the Aghora strike. Normally able of killing an entire universe, s/he's so badly weakened that she knows she can't win by fighting an army of clones. So she has take a desperation plan that has her sacrificing her quasi-magical sword to fly up into orbit and nuke the planet from safe distance. No other Metabaron except the first who was dying from an incurable disease, has ever shown that level of 'weakness'.
- In the Historical Fiction graphic novel The Borgias, one nobleman's unfaithful, heavily pregnant wife is shown enjoying a passionate encounter with her lover before they're both Impaled with Extreme Prejudice by an assassin.
- Blacksad: In the Arctic Nation album, it is eventually revealed that Chief Karup's greatest crime after he became a white supremacist was to drag his heavily pregnant black wife out of her bed in the middle of the night and leave her behind in the cold winter woods to die. She survived, and his children would grow up yearning for revenge.
- A Love Like Blood: The female werewolf is murdered by Sangrael and Luperci foot soldiers shortly before she was about to give birth to a vampire-werewolf hybrid.
- Fatale: Hank's pregnant wife Sylvia is brutally murdered by Bishop, who then frames Hank for the deed.
Fan Works
- Crucible (Mass Effect):
- A bunch of women who were pregnant with Hybrid babies were kidnapped by the Quarians for testing. Luckily for them, Garrus and Avitus managed to find them thanks to the help of one of the boyfriends.
- In Alt. Future, poor Amata suffered from this twice. The first time she lost her baby son. The second time, she was killed and her daughter didn't survive for long either. And with what Gaius found in the Quarians' labs, it was obvious that she wasn't the first mother to suffer this fate.
- The pregnant Assassins in A.A. Pessimal's Discworld fic Hyperemesis Gravidarum have got more to contend with than sore feet and morning sickness. At least one of them is on the hit-list of a murder gang bent on vengeance against her and her wider family. The battle goes to the wire.
Film — Live-Action
- Played with at the end of Taking Lives, the character played by Angelina Jolie is pregnant and gets stabbed in the belly, only to reveal the pregnancy was fake and the corner stone of an elaborate scheme to catch the killer.
- In Thursday, Casey has a flashback as to what it was that finally helped him decide to turn his life around from being a drug dealer: during a busted deal, his partner Nick repeatedly shoots an eight-month pregnant woman in the stomach, killing both the woman and the unborn baby.
- One of the Headless Horseman's victims in Sleepy Hollow. Her unborn child gets decapitated as well.
- In Gangs of New York, Jenny's baby was cut out of her after she got pregnant from Bill the Butcher, leaving a big nasty scar on her body and her traumatized
- A sixteen year old girl in The Rite was raped and impregnated by her father, causing a demon to posses her. During her exorcisms, the demon causes her body to do very disturbing and unnatural things. She is later sent to the hospital where both she and the fetus are killed by the demon.
- Jigsaw's wife in Saw IV lost their unborn son due to a door violently smashed against her belly. This was the Start of Darkness for him.
- In What to Expect When You're Expecting, Rebel Wilson's character tells her pregnant boss that her sister got herpes because of her pregnancy.
- In Aliens vs. Predator: Requiem, the Predalien is completely indiscriminate in its victims, invading a maternity ward in the town's hospital and implanting pregnant women with its own xenomorph embryos.
- In the French horror movie Inside, a pregnant woman is attacked by a stranger who wants to steal her baby. With scissors. During Christmas Eve.
- In Curse of Chucky, a pre- doll transformation Charles Lee Ray is seen stabbing a pregnant woman to cripple her unborn daughter after she rejected his advances.
- In the first Warlock movie, the Warlock threatens to kill a priest's pregnant wife along with the child she's carrying to get the information he wants. Presumably subverted because the priest ultimately caves in to the Warlock's demands, although we don't get to see if he did leave them unharmed.
- Blade's origin story from the Blade Trilogy involved his mother being attacked by a vampire when she was heavily pregnant, causing her to die on an operating table and the boy turning into a human-vampire hybrid. Blade swore to find the vampire who killed her to avenge her death. Near the end, Blade discovers that his mother is still alive after the vampire in question turned her, and it was actually Deacon Frost, the bad guy he had been pursuing the entire film.
- In Star Wars Episode III: Revenge of the Sith a heavily pregnant Padmé goes gallivanting off to Mustafar after Anakin after Obi-Wan tells her Anakin has fallen to the dark side. When Obi-Wan turns up, having stowed away on Padmé's ship, Anakin Force chokes her. Padmé starts dying afterwards, forcing medical droids to induce labor and deliver Luke and Leia.
- In Match Point, the protagonist kills his pregnant mistress because he can't choose to leave his wife and sacrifice his cushy lifestyle.
- Occurs in Mad Max: Fury Road with Angharad, who is very visibly pregnant and spends all of her screentime in horrible danger. She ends up slipping off the War Rig and run over by Immortan Joe's car. Both she and her baby end up dying as a result.
- In Who Can Kill a Child? and the remake, Come Out and Play, the female lead is 7 months pregnant at the start of the film. Her pregnancy hampers quick movement and ups the drama particularly when she starts believing that the fetus has gone murderous as well. And she might be right.
- The 2002 thriller Angel Blade features a Serial Killer who deliberately targets prostitutes who have gotten pregnant. His Freudian Excuse: After the accidental death of his own pregnant wife, he feels compelled to kill women he deems "unworthy" of pregnancy.
- At the beginning of The Sharing Knife, protagonist Fawn has left her home to avoid being shamed for her unwed pregnancy. Then an Eldritch Abomination ground-rips (tears the life-force out of) her unborn child. Things do get better for her, but she has one hell of a woobie-fied start.
- Discworld has multiple examples throughout the series, though it's often combined with Pregnant Badass.
- It turns out that Sybil Vimes, fairly early in her pregnancy, escapes a locked room, climbs out a window on knotted bedsheets and wallops a werewolf with an iron bar in The Fifth Elephant. She also stands up rather bravely under an assault by murderous bandits while on the road to Uberwald, holding it together while being held hostage.
- In Raising Steam, the Low King of the Dwarves, Rhys Rhysson, who later styles herself Queen Blodwen, is targeted for political assassination.
- And I Shall Wear Midnight has a decidedly dark example as Amber Petty is beaten by her father, causing a miscarriage/stillbirth.
- In The Hunger Games: Catching Fire, while Katniss wasn't actually pregnant, Peeta manages to enrage the capitol citizens by claiming that she was right before the 75th Games started, trying to invoke this trope.
- Possibly Annie when she was held captive and tortured by the Capitol in Mockingjay, since we don't know when her and Finnick's child was concieved.
- Barrayar:
- Alys Vorpatril, while pregnant with the baby Ivan Vorpatril, is stranded in a besieged city held by a pretender desperately looking for distinguished hostages like Alys.
- Cordelia is attacked with a poisonous gas while pregnant. The aftereffects of the treatment that saved her life drive most of her son's life: Miles, the protagonist of most of the books, was dwarfed and crippled by the effects.
- Carla Cassidy's Pregnesia: Amnesiac widowed heroine, Good Samaritan hero, bad guys after the baby.
- This is teased with Elayne in The Wheel of Time, who gets impregnated in the ninth book. Thanks to a prophetic viewing, she is convinced her children can't be harmed and gets into trouble several times, finally coming to her senses after a particular wound runs dangerously close to her womb. Then things get brutal: during the climax of the last book, she runs afoul of a minor villain from previous books who intends to cut out her unborn twins and use magic to keep them alive until they can be offered to the Big Bad. He promises to rape her afterward. A timely plot device saves the day, however.
- Celine Herondale from The Mortal Instruments, committed suicide while pregnant.
- Gone Girl: It's eventually revealed that Amy was pregnant when she disappeared
not. She was deliberately invoking this trope to create media hysteria.
I knew the key to big-time coverage, round-the-clock, frantic, bloodlust never-ending Ellen Abbott coverage, would be the pregnancy. Amazing Amy is tempting as it is; Amazing Amy knocked up is irresistible. Americans like what is easy, and it's easy to like pregnant women; they're like ducklings or bunnies or dogs.
- A Rizzoli & Isles novel has an escaped convict taking a maternity ward hostage. Among the patients is Jane Rizzoli. She's never harmed.
Live-Action TV
- In the second episode of the short-lived 2000 ABC series Wonderland, a pregnant doctor gets stabbed in the stomach with a syringe by a mental patient.
- Played with in Desperate Housewives. A pregnant Bree gets stabbed in the belly. However, no blood appears because it's a fake belly. But it does threaten to uncover the truth.
- In the Star Trek: The Original Series episode "Friday's Child", a usurper named Ma'ab kills Aka'ar, the Teer (tribal king), in an attempted coup. He then demands Aka'ar's pregnant wife Eleen and her unborn son killed, as the unborn son is the true heir of succession, and Kirk, Spock and McCoy have to go on the run with Eleen to keep her safe.
- Star Trek: Deep Space Nine
- In "Body Parts" Keiko O'Brien is badly injured in a shuttle accident, and Dr. Bashir is forced to transplant the unborn Kirayoshi O'Brien into Kira Nerys, the only other female present. In this case it was an Enforced Trope: In real life, Nana Visitor (Kira) was married to Alexander Siddig (Bashir) and had gotten pregnant with his baby, and rather than find some way to Hide Your Pregnancy they wrote it into the script.
- In "The Darkness and the Light" a Serial Killer targets the surviving members of Kira's old Bajoran resistance cell, eventually luring the very pregnant Kira to his hideout, stunning her, and then attempting to cut Kirayoshi out of her (the intent was to save the innocent baby and take revenge on Kira). She manages to turn the tables and shoot him.
- The NCIS Christmas Episode "Newborn King" had the team defending a very pregnant Marine from mercenaries trying to get the baby, due to it being, if male, the only surviving heir to an Afghan tribe. (She'd fallen in love with the previous heir, who was later killed by the Taliban.) A Maternity Crisis ensues and Gibbs ends up delivering the baby in a gas station while Ziva fights off the mercs in the next room.
- Game of Thrones:
- A pregnant character is stabbed multiple times in the belly at one point, leading to the death of both her and her child. For bonus points, the character is a Canon Foreignernote so the producers only added the pregnancy storyline for drama and gore points.
- Daenerys is imperiled multiple times during her pregnancy, first by her brother, which completely breaks their tenuous familial bond and later by a wine merchant who seeks to poison her.
- Stargate Atlantis:
- As Teyla neared the end of her pregnancy, Michael kidnapped her to use her son as a testbed for his human-wraith hybrids. One Alternate Universe shows that had he finished his experiments, he would have created an army that would have effectively taken over the Pegasus Galaxy, but Teyla never saw it. In the proper time, the team manages to save Teyla and her baby.
- In another episode, Teyla is using her limited psychic skills gained from her Wraith DNA to temporarily mind control a particularly strong Wraith Queen, who are the psychic heavyweights of the vampiric race. Trapped in her own mind, the Queen wonders how Teyla is suddenly so strong that she could pose a challenge for her until she realizes that Teyla's unborn son is helping her, and the Queen nearly kills the child in return.
- Doctor Who:
- Amy is once kidnapped while pregnant because the Silence's boss thinks that her unborn child could be a Time Lord. Not to mention all of the situations she ends up getting into with the Doctor, including her husband dying. Something like ten times.
- In the episode "Amy's Choice", one fake version of Amy is pregnant and trapped in a small town with aliens disguised as the elderly (again) ready to disintegrate everyone, but mostly her family. To get away, she drives a van into a cottage. And her husband dies. Three times. In one episode.
- In Grimm, one of Sgt. Wu's Filipino friends gets attacked by an aswang, which nearly kills her fetus while she was sleeping. Nick and Hank were able to resolve the situation and save her again from the monster.
- In Sons of Anarchy, Tara is kidnapped in the second season. When one of the kidnappers tries to kick her in the stomach, she begs him not to because she is pregnant. He stops and hits her in the face instead.
- Rizzoli & Isles has Jane helping a teenage witness survive while trapped in a warehouse with a hitman. She is far from delicate and survives many perilous situations while pregnant, but Jane later loses the baby after the hitman hits her in the stomach with a pipe.
- In the pilot episode of Boardwalk Empire, a pregnant Margaret is beaten so severely by her Domestic Abuser husband that she miscarries.
- Unsere Mütter, unsere Väter: Aspiring actress Greta begins an affair with a Gestapo officer to protect her Jewish boyfriend. Later in the war she confronts him by revealing that he has gotten her pregnant and should provide for his out-of-wedlock child. He responds by beating her so severely that she miscarries, then has her arrested.
- Containment features a nine-months-pregnant teenager who is trapped in the cordoned off section of the city with the virus that kills people within 48 hours.
- The second season of Veronica Mars starts with a bus crash that kills everyone on board except for Meg Manning who's later discovered to be pregnant. She later succumbs to her injuries, but they're able to save the baby.
- In the Marple episode "The Blue Geranium", a six-months-pregnant woman is strangled...and that isn't even the primary mystery!
- In one episode of Law & Order: Special Victims Unit, where Creator/Martinshort plays the villain of the week, it's revealed that one character is not her child's mother at all. She assaulted a woman very close to giving birth, and cut the unborn fetus out of her body to steal it, killing her in the process. The child is returned to his grieving father by the end of the episode.
Professional Wrestling
- When Lita was carrying Kane's baby, they ended the angle when Gene Snitsky knocked Kane into Lita which made her fall off the apron of the ring, losing the baby.
- In IWA Puerto Rico, Genesis had a habit of ducking out of matches with La Amazona. She was injured, she was sick, ect. When she came to Amazona with "I'm pregnant", well Amazona did not seem to believe her, slapping Genesis after giving her congrats, causing EMTs to swarm to Genesis's aid before Amazona could do anything else
- Later that same year (2008), Portia Perez went after mom to be and SHIMMER co founder Allison Danger. Unlike La Amazona, there was no mitigating circumstance, Perez knew exactly what she was doing, taking the opportunity to end the career of a rival (who was still nursing a collar bone broken by Cheerleader Melissa) and aiming to end something else along with it. This incident is also largely why the Eagles Club Audience loves referee Bryce Remsburg.
- Thankfully subverted the next year in WSU when Princess Jana snapped and beat the stuffing out of her Soul Sisters partner Luscious Latasha, who had revealed she had been avoiding tagging in because she discovered she was pregnant. After getting back from the hospital it was revealed Latasha was not pregnant. Latasha was still upset with Jana because she wasn't lying, just mistaken.
Video Games
- Star Trek Online: In the Foundry mission "Bait and Switch" one of the slaves kidnapped by the Orion Syndicate is a six-months-pregnant Romulan. She and her husband get rescued by the Player Character.
- In the opening of Valkyria Chronicles Martha is about nine months pregnant when the Empire invades Bruhl, and she and Isara held at gunpoint by a pair of Imperial troopers. Welkin and Isara manage to kill both troopers, but naturally the baby chooses that exact moment to show up.
- Mass Effect: Andromeda: The conclusion to one questline involves you tracking down the woman pregnant with the first human child to be born in the Andromeda galaxy, in the hopes of averting this trope This turns into a Maternity Crisis when you catch up to her and find that she's in labor, just in time for a kett battalion to show up followed by a Roekarr ambush. Thankfully, the child lives.


text:
The kitchen is statistically the most dangerous room in a home because it contains the highest concentration of knives, open flames, and pots full of semi-living lobsters. Learn to navigate this danger zone with today's Groupon: for $59, you get a cooking class for two at Senses! Recreational Cooking School (a $130 value).
Led by executive chef and seasoned culinary coach Beverly Lee, Senses! helps kitchen novices and skilled cooks alike increase their cooking savvy with an array of fun, engaging classes. Chef Bev engages students in an interactive environment, performing demonstration-based classes for hands-free learning, while hands-on classes make good use of limbs recently replaced with meat thermometers. Cuisine apprentices can soak up stewing, searing, and saucing skills from seminars on international cuisine, healthy cooking, gourmet cooking made easy, and more, before feasting upon the final product. All sessions include tutelage on concocting a savory two- to three-course meal, a pair of spiffy signature Senses! chef's aprons (yours to take home), and a professional menu with step-by-step instructions. While all necessary cooking equipment and ingredients will be provided, students should plan to bring a pen and paper for jotting down helpful recipe notes and tips for destroying meals that become self-aware.
Chef Beverly Lee of Senses! Recreational Cooking School has more than 690 fans on Facebook.

subset: commoncrawl


text:
Juke Box Hero Lou Gramm comes to The Flying Monkey
Plymouth, NH. At 7:30pm on Saturday, April 19th, The Flying Monkey presents an evening of Foreigner music with former frontman Lou Gramm. Lou Gramm has garnered critical and fan praise for writing and performing some of the rock's biggest hits both with Foreigner and with his own band. Fan favorite Charlie Farren will open the night. Tickets for Lou Gramm start at $59.
Lou Gramm rightly bills himself as the voice of Foreigner, having contributed his impassioned vocals to hits such as, "I Want to Know What Love Is," "Urgent," "Hot Blooded" and "Cold as Ice," and many more.
For legal reasons, however, Gramm can't tour under the band's name. Foreigner is controlled by founder Mick Jones, who has assembled a roster of replacement musicians but is rarely part of the touring lineup these days because of his poor health.
Gramm was the lead vocalist on all of Foreigner's hit songs, including "Feels Like the First Time", "Cold as Ice", "Long, Long Way from Home", "Hot Blooded", "Double Vision", "Blue Morning, Blue Day", "Head Games", "Dirty White Boy", "Urgent", "Juke Box Hero", "Break It Up" and "Say You Will". He co-wrote most of the songs for the band, which achieved two of its biggest hits with the ballads "Waiting for a Girl Like You", and "I Want to Know What Love Is", which was a #1 hit internationally in 85'. The latter was credited only to Jones; however, Gramm indicated that he contributed to half of its writing. Gramm's solo hits include songs like "Midnight Blue" and "Just Between You and Me."
Expect an evening that will catapult fans back to Foreigner's heyday with a remarkable show including all the hits- performed with the intensity and passionate voice that made them famous in the first place.
Tickets for Lou Gramm are on sale now for $59, and $69 for Premier seats. For tickets and more information on concerts and the Flying Monkey Performance Center, visit www.flyingmonkeynh.com or call 603-536-2551.


text:
Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
As each tradesman expresses interest in quoting on your job, we'll send you their profile and ratings to help you choose the right one for you.
After the work is complete, return to the site and rate your tradesman based on their quality of work, reliability and value for money.
Gardener / Garden Designer
Added 05/07/2013
Need to get quotes to trim back hadges in front and back gardens. Garden waste to be disposed...
Gardener / Garden Designer
Added 05/03/2013
I need a new patio laying. This is to be over-laid onto of an existing old patio area so there...
Gardener / Garden Designer
Added 17/08/2012
I need some big hedges trimmed and a lot of brambles/ivey taken out of them. I also need an...
Gardener / Garden Designer
Added 01/11/2009
wood, to build wooden raised beds
Excellent throughout. Clear quote well presented, open always to questions. Work carried out on time, and as required. Would not hesitate in recommeding, one of the best tradesman I have dealt with
Very honest, our fence had been blown over, David suggested repairing the panels rather than replacing with new. Would highly recommend. Chelmsford May 2015.
Jason and his team were absolutely brilliant at transforming our field into a beautiful garden. They were extremely professional at all times and their work was done to an extremely high standard. Nothing was too much trouble. Would certainly recommend their services.
Good communication. Supplied list of materials required. arrived on time and delivered perfect work within the quote.
Managing your job is easier with the Rated People Homes App, download it today.


text:
Genetic variation in SPAG16 regions encoding the WD40 repeats is not associated with reduced sperm motility and axonemal defects in a population of infertile males
- First Online:
- Received:
- Accepted:
- Citations
- 2.5k Downloads
SPAG16 is a critical structural component of motile cilia and flagella. In the eukaryotic unicellular algae Chlamydomonas, loss of gene function causes flagellar paralysis and prevents assembly of the "9 + 2" axoneme central pair. In mice, we have previously shown that loss of Spag16 gene function causes male infertility and severe sperm motility defects. We have also reported that a heterozygous mutation of the human SPAG16 gene reduces stability of the sperm axonemal central apparatus.
In the present study, we analyzed DNA samples from 60 infertile male volunteers of Western European (Italian) origin, to search for novel SPAG16 gene mutations, and to determine whether increased prevalence of SPAG16 single nucleotide polymorphisms (SNPs) was associated with infertility phenotypes. Semen parameters were evaluated by light microscopy and sperm morphology was comprehensively analyzed by transmission electron microscopy (TEM).
For gene analysis, sequences were generated covering exons encoding the conserved WD40 repeat region of the SPAG16 protein and the flanking splice junctions. No novel mutations were found, and the four SNPs in the assessed gene region were present at expected frequencies. The minor alleles were not associated with any assessed sperm parameter in the sample population.
Analysis of the SPAG16 regions encoding the conserved WD repeats revealed no evidence for association of mutations or genetic variation with sperm motility and ultrastructural sperm characteristics in a cohort of Italian infertile males.
KeywordsSperm ultrastructure Axoneme Motile cilia Male infertility Central apparatus Semen analysis
- SNPs
Single nucleotide polymorphisms
Primary ciliary dyskinesias
Transmission electron microscopy
Untranslated region
Linkage disequilibrium.
Infertility impacts approximately 9% of couples globally, with reports ranging from 3.5% - 16.7% of couples [1], and it is estimated that male factor infertility plays a role in as many as 55% of cases [2]. There are multiple causes of male infertility, including congenital factors and environmental exposures [3, 4, 5], as well as gene mutations which cause defects in spermatogenesis and sperm flagellar dysfunction.
The most studied mutations associated with abnormal sperm motility and male infertility cause the primary ciliary dyskinesias (PCD) [6, 7]. Genes involved in the structure and function of the "9 + 2" axoneme, the principle scaffold and regulator of motile cilia and flagella, have been extensively studied and characterized in the biflagellate eukaryotic algae Chlamydomonas, wherein loss of axonemal components has been shown to cause immotility [8, 9]. Most of the mutations identified to date that cause PCD in humans encode proteins that are associated with the axoneme outer doublets. However, relatively little is known about the contribution of mutations or genetic variation in genes encoding the central apparatus of the axoneme to sperm motility defects. It is known that the "9 + 2" axoneme is strongly conserved in overall structure and function [10, 11].
We previously reported that mice lacking Spag6, which encodes the mammalian orthologue of the Chlamydomonas axonemal central apparatus protein PF16, exhibited a phenotype of both male infertility associated with severe sperm motility defects and axonemal structural abnormalities [12], demonstrating that loss of an axonemal central apparatus protein can have dramatic effects in mammals as well.
SPAG16, the mammalian orthologue of the essential Chlamydomonas central apparatus protein PF20 [13, 14], is also required for male fertility and sperm motility in mice [15]. The Spag16 gene encodes two major transcripts in mice, which generate SPAG16L, a central apparatus protein, and SPAG16S, a protein localized to the male germ cell nucleus and cytoplasm. Male mice homozygous for a mutation ablating SPAG16L production have a sperm motility defect and are infertile [15]. The axonemes of SPAG16L-deficient mice also show abnormal responses to calcium, indicating that loss of SPAG16L disrupts either the ability to process or the ability to respond to key molecular signaling [16]. When the transcripts encoding SPAG16L and SPAG16S are both disrupted in a transgenic male mouse, there is haploinsufficiency and abnormal spermatogenesis in the chimeric state, implicating SPAG16S in fundamental processes of sperm formation distinct from the structural role of SPAG16L [17]. Importantly, the Spag16 mutant allele affecting both isoforms was not transmitted to offspring by chimeric males, demonstrating that heterozygous/non-homozygous Spag16 mutation may cause a pronounced phenotype, and that the two murine SPAG16 isoforms each play different but essential roles.
We have also reported previously that sperm from human subjects heterozygous for a frame shift mutation in SPAG16 exhibited instability of important central apparatus components SPAG16L, SPAG17, and SPAG6 [18]. While both subjects in this report were fertile, observed biochemical instability of the sperm axoneme suggests that even heterozygous disruption of SPAG16 has phenotypic consequences that may reduce fecundity. Additionally, a preliminary study of oligozoospermic and oligoasthenozoospermic males has identified 29 previously unreported mutations in SPAG16 transcripts isolated from ejaculated sperm, further suggesting a critical role for SPAG16 in male fertility [19].
In the present study, 60 males of Western European (Italian) origin volunteered for semen and genomic analyses following diagnosis of male factor infertility. We evaluated the genomic region encoding the highly conserved WD domains of SPAG16 proteins (exons 10–16). WD regions are semi-conserved 20 amino acid sequences giving rise to a β-propeller tertiary structure. These domains are the only known functional domains found in SPAG16 proteins, both isoforms of which contain seven such domains. These β-propeller structures are classically thought to mediate protein-protein interactions (reviewed in [20]), playing crucial roles in macromolecular assembly relevant to a diverse array of cellular tasks, including cell growth and division, intracellular communication, apoptosis, and transcription regulation [21]. Recent reports have also demonstrated that some WD domains interact directly with specific RNA sequences, further expanding the range of potential roles played by members of this class of proteins [22].
To better understand potential links between SPAG16 gene variation and specific mechanisms of infertility, semen analysis was performed for each subject [23]. In addition, sperm ultrastructure was assessed by transmission electron microscopy (TEM) and described according to previously established standards [24, 25].
Based on our previous observations of Spag16 gene function in mice, and the influence of a heterozygous mutation affecting the WD repeat region in humans on central apparatus stability, we hypothesized that functional SNPs in the human SPAG16 gene could cause defects in sperm motility and ultrastructure manifest in both the heterozygous and the homozygous states. The present study analyzed genetic variation in a population of infertile males, and their association with parameters that are potentially regulated by SPAG16.
SPAG16 sequences are highly conserved
SPAG16 amino acid sequence conservation analysis
Homo sapiens
Mus musculus
Bos taurus
(Pred) Gallus gallus
(Pred) Pan troglo-dytes
Chlamydomonas reinhardtii
Similarity Scores (%)
Homo sapiens
Mus musculus
Bos taurus
(Predicted) Pan troglodytes
Chlamydomonas reinhardtii
Identity Scores (%)
Analyzed SPAG16 SNPs allele frequencies are not altered in the sample population
For one SNP analyzed by Taqman Genotyping Assay, rs10167688, no individuals with the minor allele were detected. This result was further corroborated by exon region-specific sequencing. Heterozygosity for this SNP has been reported only in West African Yoruban populations [26], so its absence in our population was not unexpected. The probe set used to analyze this SNP was confirmed to be effective using a test set of West African female DNA samples obtained in an unrelated study (data not shown).
SPAG16SNP distributions in sample population
C [exon 11 3′ + 15]G
G [exon 14 3′ +25] A
Homozygous Major allele
Homozygous Minor allele
Sample population Minor allele frequency
HapMap Minor allele frequency
Analysis of linkage disequilibrium in exon 11 SNPs
Analyzed SPAG16 SNPs are not associated with sperm parameters and alterations of axonemal or periaxonemal ultrastructure, fertility index, and sperm pathologies
In the infertile male population studied, semen analysis revealed severe defects in sperm concentration, motility, and ultrastructure (full data in Additional file 2: Table S2). No single parameter was uniformly perturbed amongst all patients carrying a particular SNP minor allele. Additionally, no mutation was found for which all homo- or heterozygous carriers displayed a similar phenotype beyond deficiencies common to the sample (infertile) population in general. There were nominally significant associations found between specific SNPs and sperm ultrastructural characteristics, but these were not significant after Bonferroni correction for multiple testing. Minor allele carriage at rs16851495 correlated negatively with normal axoneme structures (β = −0.16, nominal p < 0.05; corrected p > 0.1). Minor allele carriage at rs12623569 showed a small, nominally significant, positive correlation with normal fibrous sheath structures (β = 0.09, nominal p < 0.05; corrected p > 0.1) (see Additional file 3: Table S3).
For two SNPs [dbSNP: rs2042791; dbSNP rs2042792] in LD, haplotype was not associated with studied sperm parameters or ultrastructural variation (Additional file 4: Table S4).
Amino acid-altering SPAG16 SNPs are predicted to be functionally tolerated
Two of the identified SNPs cause point mutations in SPAG16 proteins. These amino acid substitutions were assessed for predicted effects on protein structure and function. Both amino acid substitutions were predicted to be tolerated using SIFT [27], which analyzes amino acid properties and conserved identities.
In the SPAG16 regions analyzed, both major and minor alleles were present in the study group for four known polymorphisms, and no previously unreported mutations were detected. At rs2042791 [29], the minor allele replacement of an adenosine by a cytosine residue causes the replacement of a glutamine at position 361 [GenBank: NP_078808.3] with a histidine. Previous genotyping studies have reported a minor allele frequency of 0.39 [26] in individuals of Caucasian ancestry for this SNP. Fisher's exact test demonstrated no significant difference between the measured minor allele frequency of 0.408 in our sample population and that established in control populations (p > 0.05). While the present study lacks sufficient power to detect small effects of these alleles on variation in observed parameters of sperm characterization, among patients carrying this SNP no single factor was uniformly deficient within the distribution of sub-normal semen analysis of the study participants. Full genotype and semen analysis results are included for reference (Additional file 2: Table S2).
The lack of association between this SNP and sperm dysfunction is not surprising, given that the glutamine replacement by histidine is predicted to be tolerated in the protein structure [27]. Further, the amino acid is not conserved in mammals – Mus musculus [GenBank:AAI20669.1, NP_080004.1, NP_083436.2] and Rattus norvegicus [GenBank:NP_001128200.1, AAI58603.1] reference sequences for the orthologous proteins in fact report a histidine at this position, consistent with the human minor allele. The same amino acid replacement is predicted in Pan troglodytes [GenBank:XP_001148592.1, XP_526016.2, XP_001148393.1]. These data are consistent with the hypothesis that structural rather than sequence homology is essential to maintenance of axoneme function.
The rs2042792 minor allele was not found at a significantly different frequency than in the reference population (p > 0.05), and did not correlate with assessed sperm phenotypes.
Rs2042791 exists in LD with rs2042792 in our population, but no phenotypic consequences were associated with any of the observed haplotypes.
Minor allele replacement of an adenosine with a cytosine in rs12623569 causes an amino acid replacement at position 425 [GenBank:NP_078808.3], with a threonine taking the place of a lysine. Previous genotyping studies have shown a minor allele frequency for this SNP in individuals of Caucasian ancestry of 0.30 [26]. The present sample population exhibited a minor allele frequency of 0.333, which was not significantly different than that in control populations (p > 0.05). While the lysine at position 425 encoded by the major allele is present in other mammals (Mus musculus – GenBank:NP_080004.1, NP_083436.2; Rattus norvegicus – GenBank:NP_001128200.1, AAI58603.1), replacement by threonine is predicted to be tolerated by the protein structure. Interestingly, minor allele carriage was found to be nominally associated with a higher percentage of normal accessory fibers of the axoneme. Although the statistical significance of this association was lost upon correction for multiple testing, further studies on a larger study population may be warranted to identify contributions of the SNP to sperm flagellar structure.
At rs16851495, minor allele replacement of guanine with an adenine residue does not affect protein translation directly, as the position lies outside the translated exon region. However, minor allele carriage was nominally associated with the reduced presence of normal axoneme structure in the study population. The statistical significance of this association did not withstand correction for multiple testing. However, the limited power of our study may have contributed to the absence of a robust association.
For all SNPs discussed, both heterozygous and homozygous individuals were present in the sample population, and statistical analysis demonstrated no difference in genotype frequency between the sample and control populations (p > 0.05). While the study did not have sufficient power to analyze possible contributions to complex traits, the results negate the hypothesis that amino acid modification at the tested positions may result in a loss of protein function that would mimic the severe phenotypes observed in transgenic mouse studies.
Our results suggest that non-synonymous amino acid substitutions at residues 361 and 425 in the SPAG16 protein are not sufficient to explain a reduction of sperm motility and fertility index, the presence of axonemal/periaxonemal alterations, or an increased percentage of sperm pathologies in the assessed patient population. Based on the strong homology between members of the WD protein family, it is tempting to suspect that these resides may be non-essential in other WD repeat proteins as well.
The profound defects observed in studies of Spag16 gene effects in other species suggest that a functional mutation would significantly perturb sperm function, and would be observed even in a small sample population. Our observations do not, however, preclude the possibility that alternative variations in the SPAG16 gene cause an increase in sperm ultrastructural alterations, and a reduction in sperm motility. Indeed, our previous studies suggest that more significant SPAG16 mutations, such as a frame shift, can reduce sperm central apparatus stability. Further studies of SPAG16 gene variation are warranted to offer a comprehensive understanding of the gene's contributions to male fertility. The sensitivity of the present study to detect variation with the sample is limited by the lack of similarly extensive sperm analysis data, in particular TEM ultrastructural analysis, from a control population. Future development of this area of research should include control samples from men with known fertility and/or sperm count, motility, and structure in the normal range.
Future studies in mammals are necessary as well to explore the nature of Spag16 gene evolution, and the possibility that male germ cell-specific isoforms exist in multiple species. The only identified rat SPAG16 protein is similar to SPAG16S in size, and is derived from mRNA similar to murine SPAG16S, with a 5′UTR from the region upstream of the first exon suggestive of independent transcription, rather than splice variation. A putative SPAG16S promoter and 5′UTR have been identified in humans as well [GenBank: EF591776.1], raising the possibility that SPAG16S maybe be common to mammalian male germ cells. We have recently shown that murine SPAG16S is enriched within nuclear speckles of male germ cells [30]. This unique localization, combined with phenotypic results from various Spag16 transgenic mice, strongly suggests a unique processing role for the SPAG16S protein, and thus a distinct role for the Spag16 gene apart from its structural role in the axoneme.
In a sample population of 60 infertile males of Western European origin, mutations in SPAG16 were not significantly associated with a single phenotype of sperm alteration. The findings suggest that mutations and or genetic variation in the SPAG16 regions encoding the protein's WD repeats are not likely to be major causes of sperm motility and sperm flagellar defects.
DNA sample acquisition
DNA used in the present study was isolated from peripheral blood lymphocytes submitted by 60 male volunteers of Western European (Italian) ancestry diagnosed clinically as sub- or in- fertile. Inclusion criteria for patients were: sperm progressive motility ≤ 10%, normal lymphocyte karyotype, normal hormone levels, absence of anatomical pathologies, genitourinary infections. Patients with known ultrastructural sperm defects of possible genetic origin (dysplasia of the fibrous sheath, cilia immotile syndrome, round headed sperm, etc.) were also excluded.
Research was carried out in full compliance with the Helsinki Declaration of ethical principles for medical research involving human subjects. All patients signed a declaration of informed consent to participate in the research. The work was approved of and performed in full accordance with policies governing human subject research at the by the Ethics Committee for the University of Siena (CEL-AOUS) for specimens collected by non-invasive methods for clinical analysis, and the Virginia Commonwealth University Institutional Review Board.
Allelic genotyping was performed using Applied Biosystems TaqMan SNP Genotyping Assays according to manufacturer's instructions. Specific allelic discrimination probes and primer sets were ordered for the following 3 SNPs: dbSNP: rs2042791, rs12623569, rs10167688. Results were compared to allele frequency detected in NCBI and HapMap studies [26, 29].
Genotype was confirmed by direct sequencing. Briefly, exon plus flanking regions were amplified by PCR and sequenced using tested primers. Primer suitability for sequencing was tested by comparing results to sample PCR products digested and amplified in a Topo TA Vector (Invitrogen).
PCR and Sequencing Primers
Ex10 – Forward: 5′ - TTCATGTAAATTCTGGGCAAA - 3′; Reverse: 5′ - GCAAACCATTTCAACCATGA - 3′
Ex11 – Forward: 5′ - TGGGGCCAGTACTCTCAAAA - 3′; Reverse: 5′ - TTCAGTGCAGGGTGTGTTGT - 3′
Ex12 – Forward: 5′ - GCAATTCAAGTTAGCAATTGTG - 3′; Reverse: 5′ - CCTGGGGTAGCATCAAGG - 3′
Ex13 – Forward: 5′ - TTATTTCATGCCTCAGTTCCTT - 3′; Reverse: 5′ - GCCCTTGCACAATCACTTTT - 3′
Ex14 – Forward: 5′ - GGGAGGAGGGGCTAAAAATTA - 3′; Reverse: 5′ - CCTAAAGTTGTTCTTCTCACCTCA - 3′
Ex15 – Forward: 5′ - AGAGGAATGTAATCTTATGGCTGT - 3′; Reverse: 5′ - TTCATATGACATGCTATACGTAATGA - 3′
Ex16 – Forward: 5′ - CTGACCCCTAACACAGAATGA - 3′; Reverse: 5′ - CCAGGTTTTCCTGCAGTTT - 3′.
Statistical analysis
Fisher's exact tests implemented in the open-source R software package were used to test for differences between allele frequencies observed in the sample population and those reported in HapMap [31]. Hardy-Weinberg equilibrium tests and inter-SNP linkage disequilibrium calculations were performed using PLINK [31]. Haplotypes were assigned to subject based on the most likely phase reconstructed haplotype generated by the expectation-maximization algorithm implemented in PLINK. Haplotypes were then imported to R and an additive term for the haplotype of interested was coded as 0, 1, or 2 based on copy number present. Single SNP and haplotype associations with all phenotypes were carried out using multiple logistic regression in R. Phenotypes measured as percentages were transformed with arcsine of the square root of the percentage to stabilize the differences in variance between samples. The Fertility Index is a standard measure ranging from 0 to 100+. All values greater than 100 were coded as 100. To achieve a more normal distribution and minimize residual errors, sperm/mL was analyzed using a log transformation. A Bonferroni p value was calculated to correct for multiple testing error.
Sperm analysis
Semen samples of patients were collected by masturbation after 4 days of sexual abstinence and examined after liquefaction for 30 min at 37°C. Volume, pH, concentration (sperm x 106/mL) and total motility (a, rapid + b, slow, + in situ) were evaluated according to World Health Organization guidelines [23]. For electron microscopy, sperm samples were prepared as previously described [24, 25].
In each sample the percentage of normal axonemal pattern, of coiled axonemes, of well-assembled fibrous sheaths and accessory fibers were estimated by TEM. Moreover, all TEM data were elaborated using the statistical mathematical formula by Baccetti et al. [25], based on the Bayesian method, which calculates the number of spermatozoa probably free of structural defects (fertility index, FI) and the percentages of three main phenotypic sperm pathologies: immaturity, necrosis and apoptosis [24].
Molecular structure prediction
Using SWISS-MODEL [28], mouse SPAG16L was evaluated for homology with proteins of known structure, and the results mapped on the 3-D structure of the most closely related homologue.
The molecular analysis tool SIFT [27] was used to predict whether the analyzed amino acid substitutions would be tolerated, based on sequence homology and amino acid physical properties.
Amino acid alignment
Alignment analysis, including scoring of conservative versus semi-conservative or radical amino acid substitutions, was performed using recommended default settings in MacVector 11.6 (MacVector. Inc.; Cary, NC) for the following isoforms:
Homo sapiens – GenBank:NP_078808.3
Mus musculus – GenBank:NP_083436.2
Bos taurus - GenBank:DAA32462.1
Gallus gallus - GenBank:XP_421865.2 (predicted)
Pan troglodytes - GenBank: XP_001148592.1 (predicted)
Chlamydomonas reinhardtii - GenBank:AAB41727.1
Micromonas sp. RCC299 - GenBank:ACO61299
Maximum likelihood tree
Amino acids sequences were aligned by Clustal W [32] using default settings, hosted by GenomeNet at the Kyoto University Bioinformatics Center [33]. Aligned sequences were analyzed for maximum likelihood tree and formatted for figure output using programs in the Phylip 3.69 software package [34]. Isoforms used were as follows:
C. reinhardtii – GenBank:AAB41727.1
M. pusilla CCMP1545 – GenBank:EEH57835.1
E. siliculosis – GenBank:CBN79843.1
P. patens – GenBank:EDQ58898.1
T. brucei – GenBank:AAC83819.1
T. gondii ME49 – GenBank:EEA99732.1
(Rooster) G. gallus – GenBank:XP_421865.3 (Predicted)
(Opossum) M. domestica - GenBank:XP_003341903.1 (Predicted)
(Macaque f.) M. fascicularis – GenBank:BAE01109.1
(Rhesus macaque) M. mulatta – GenBank:XP_001082826.2 (Predicted)
(Gibbon) N. leucogenys - GenBank:XP_003254083.1 (Predicted)
(Chimpanzee) P. troglodytes - GenBank:XP_001148592.1 (Predicted)
(Human) H. sapiens N-terminal variant – GenBank:NP_001020607.1
(Human) H. sapiens SPAG16L – GenBank:NP_078808.3
(Bull) B. taurus - GenBank:DAA32462.1
(Rat) R. norvegicus (discovered in testis; SPAG16S homologue) – GenBank:AAI58603.1
(Mouse) M. musculus SPAG16S – GenBank:NP_080004.1
(Mouse) M. musculus SPAG16L – GenBank:NP_083436.2.
This work has been supported by NICHD 5R01HD037416-10 (JFS), NICHD fellowship 1F31HD062314-01 (DRN-G), and Virginia Commonwealth University Presidential Research Incentive Program (PRIP) (ZZ).
Financial support
Supported by NICHD 5R01HD037416-10 (JFS), NICHD fellowship 1F31HD062314-01 (DRN-G), and Virginia Commonwealth University Presidential Research Incentive Program (PRIP) (ZZ).
Supplementary material
Copyright information
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
This game is under construction. Please excuse its informal appearance while it is being worked on. We hope to complete it as soon as possible! [1] [2] Since this is MY game, I won't allow you to edit it. HOWEVER. I'm not english, so if you think there's an error/mistake, well... go ahead. Please feedback the game, positively or negatively. -DestROY13103
-In memory of Satoru Iwata
Super Smash Bros. Collection is a duh, collection of the first 3 Smash Bros. plus some extra content for ZeroCube.
Super Smash Bros. Collection is a collection of various Smash Bros. and their remakes.
- Super Smash Bros
- Super Smash Bros. HD
- Super Smash Bros. Melee
- Super Smash Bros. Melee HD
- Super Smash Bros. Brawl
- Super Smash Bros. Brawl HD
- Project M (from Demo 1.0 to 3.6)
- Project M+
- Super Smash Bros. for 3DS Demo and Super Smash Bros. for Wii U Demo
- Super Smash Bros. for ZeroCube Demo
- Brawl + and -
- Smash Maker
Super Smash Bros. (64) & Super Smash Bros. HD
Super Smash Bros. is included.
Super Smash Bros. HD is a remake in High Definition.
New Characters: Mewtwo, Bowser, King Dedede, Meta Knight, Pit, Zelda.
New Stages: Green Greens (Melee), Venom (Melee), Onett (Melee), Port Town Aero Dive (Brawl), Pokémon Stadium (only normal form, Melee), Jungle Japes (Melee), Norfair (Brawl), Skyloft (Project M), Battlefield and Final Destination.
All-Star mode (when unlocked everyone).
Super Smash Bros. Melee & Super Smash Bros. Melee HD
Melee is included.
New Characters: King Dedede, Meta Knight, Takamaru, Mach Rider, Excitebiker, Balloon Fighter, Wario, Lucas, Pit, Olimar, Wolf, Ridley.
New Stages: Dedede Arena (ZeroCube), Akaneia (?), Castle Siege (Brawl), Past Stages: Hyrule Castle, Past Stages: Mushroom Kingdom, Past Stages: Congo Jungle, Past Stages: Peach's Castle, Past Stages: Planet Zebes, Past Stages: Saffron City
New Stuff: Board the Platforms and Race to the Finish 64 brought back in the stadium. Also Race to the Finish Melee can be played in the stadium as well.


text:
To the Editor:
I have been observing the comments regarding the Ten Commandments monument placed in the Alabama State Supreme Court lobby (news article, Aug. 23).
Why can the federal court say anything about the Ten Commandments being in a state building? Every courtroom has a Bible to swear in witnesses and juries. The Bible certainly has the Ten Commandments in it.
Fate, Tex., Aug. 23, 2003Continue reading the main story


text:
Delta State Poly Ozoro Post-UTME Form 2017, Exam Date, Cut-off Mark, Eligibility And Registration Details is Out
Delta State Poly Ozoro Post-UTME Form 2017, Exam Date, Cut-off Mark, Eligibility And Registration Details is Out. This is to inform the general public that the Delta State Polytechnic Ozoro has announced application for 2016/2017 post-UTME is on sale.
Delta State Poly Ozoro Post-UTME Eligibility for Participation
Delta State Polytechnic, Otefe-Oghara has scheduled Tuesday 6th and Wednesday 7th September, 2016 for the screening and documentation of Candidates who wrote the 2016 Unified Tertiary Matriculation Examination (UTME) for the 2016/2017 Academic Session as follows:
Courses Available
(i) Accountancy
(ii) Banking and Finance
(iii) Business Administration and Management
(iv) Mass Communication
(v) Office Technology and Management
(vi) Computer Science
(vii) Science Laboratory Technology (SLT)
(viii) Statistics
(ix) Computer Engineering
Click here to check your NEW JAMB Result now!
(x) Electrical/Electronic Engineering
(xi) Mechanical Engineering
(xii) Welding and Fabrication Technology
Candidates Eligible for the Exercise
(i) All Candidates who chose Delta State Polytechnic, Otefe-Oghara as their first or second choice and scored 180 and above in the 2016 UTME.
(ii) Candidates who chose other Polytechnic but wish to change to Delta State Polytechnic, Otefe-Oghara and scored a minimum of 180 having changed it through JAMB.
Entry Requirements
(i) Five(5) O'Level Credit Passes including Mathematics and English Language in subjects relevant to the Candidate's course of study.
(ii) The following subjects are taken as one: Economics or Commerce, Government or History or Civic Education, Biology or Agricultural Science or Fishery or Animal Husbandry, Marketing or Salesmanship, Financial Accounting or Book Keeping, Data Processing or ICT or Computer Studies or Information & Communication Technology.
CRS is accepted only for all Business/Social Science Courses.
(iii) Candidates who applied for Computer Science must have at least a pass in Physics.
(i) Log on to www.ogharapoly.edu.ngthen click on ND Application Form.
(ii) Click the payment button to pay a non-refundable screening and documentation fee of Five Thousand Naira (N5,000.00) made online with Verve or Master Debit Card (ATM Card).
(iii) Print two(2) copies of the online acknowledgement form to conclude the registration.
Day One
(Tuesday, 6th Sept., 2016)
* Computer Science
* Science Laboratory Technology
* Statistics
* Computer Engineering
* Electrical/Electronic Engineering
* Mechanical Engineering
* Welding and Fabrication Engineering
TIME: 10.00am
Day Two
(Wednesday, 7th Sept., 2016)
* Accountancy
* Banking and Finance
* Business Administration and Management
* Mass Communication
* Office Technology and Management (OTM)
Documents Required for the Screening/Documentation
Candidates for the screening are required to come with original copies of the following:
(i) JAMB Original Result Slip with Passport photograph. If you do not have this document, click here to get yours
(ii) O/L Results
(iii) Certificate of Local Government of Origin
(iv) Two recent Passport Photographs
(v) The Screening Form (Photocopy)
(vi) Birth Certificate (Age Declaration)
Closing Date
Interested Candidates should apply on or before 2nd September, 2016.
» Buy & Download Original Post UTME Past Questions and Answers (Up-to-Date):
» Get your Original JAMB Admission Letter : Click here to Apply!
» Get your JAMB Result Slip with your passport on it: Click here to Apply!
myPastQuestion.com Products and Services:
- Post UTME Past Questions = from N1,000
- JAMB Past Questions = N1,500
- WAEC/NECO Past Questions = N500 per subject
- Junior (JS33) Past Questions = N1,000 per subject
- All Scholarship Past Questions = N2,000
- JUPEB Past Questions = N2,000
- NDA Past Questions = N2,000
- NPA Past Questions = N1,500
- Job Interview Past Questions = N2,000
- WAEC Result Checker Card = N800
- NECO Result Checker Card = N800
- Post UTME Success Guide (eBook) = N1,000
- Exam Success Guide (eBook) = N1,000
- Creation of Blogs = N10,000
Tags: Delta State Poly Ozoro Post-UTME, Delta State Poly Ozoro Post-UTME Form, Post UTME


text:
He can raise people from the dead, turn water into wine and even go for a heavenly ice skating session. But Philadelphia police draw the line at Jesus accepting tips.
"Philly Jesus," aka Michael Grant, found himself in a holy war with a police officer at Philadelphia's new seasonal ice skating rink. As Jesus himself tells it, he obliged some fans to take a picture after ice skating and accepted a tip from them (Jesus doesn't charge for photos: Blessed be the poor and all that.)
People come up to me and ask me to take pics with them and for them..I tell them i Dnt charge but tips are welcome.. cop said u r solicitin — phillyjesus (@phillyjesus)
November 14, 2014
And that's when the son of God ended up in handcuffs.
Free Philly Jesus! He walks on water.......well skates. #They know not what they do. pic.twitter.com/ao27f87zOF — Nick & Jilly (@NickandJilly)
November 15, 2014
The big JC is usually the unofficial deity of Love Park with the famous Philadelphia LOVE statue. Grant started his Philly Jesus gig after he was run over by a minivan, according to his website. Grant took a detour and became a "Satanic music artist" and fell into a heroin and cocaine addiction before he hit rock bottom and had a revelation in rehab.
And unto Philadelphia, Philly Jesus was born. He has amassed quite a flock of followers, whose righteous indignation poured out on Twitter after Grant's arrest.
VERY disappointed in my hometown for harrassing @phillyjesus . I mean, he walked on water today. OK, it was frozen at the time, but hey... — Paul Cicchini (@PaulCicchini)
November 16, 2014
Philly. The place that loves ya back unless your dressed like Jesus. @phillyjesus has a lawyer and its @gibbsesq. — hugh allen (@hughallen73)
November 16, 2014
Philly Jesus is arrested and now we're all going straight to hell — Melissa (@MEListenup)
November 16, 2014
Jesus may the way, the truth and the life, but he also is no fool. Philly Jesus lawyered up pretty quickly and his attorney, Charles Gibbs, is waging a PR campaign for the Prince of Peace.
Gov't doesn't like dude dressed like Jesus' speech and persecuted him. Sound familiar? We are proud to fight for @phillyjesus — Charles Gibbs, Esq. (@gibbsesq)
November 15, 2014
Your move, Philly police. Just watch out for lightning strikes.


text:
Service Offerings
HCL Product Support Services portfolio covering Deployment services, TAC services and proactive 24 X 7 remote operations services help realize the full potential of your world class products.
Global Deployment Services
Value Delivered
- Competitive deployment services, reduced deployment cost
- Right & multi skilled resources across various technology domains in Telecom, Network, OS and Compute
- Single project management interface for overall accountability
- Center of Excellence for Deployment Support and seamless Product Handover
- Global Localized Reach through HCL Global Delivery Centers
Technical Assistance Center (TAC)
Value Delivered
- Consistent and predictable CSAT
- Increased productivity "do more with same if not withless"
- Increased efficiency "do the right way"
- Cut down on the cost of delivering Technical Services
- Capture revenue generating opportunities
- Better Knowledge Management
- Reduced case escalation to R&D team
Remote Operations Service (ROS)
Value Delivered
- High FCR%, First time Closure – 70%
- Measure and improve CSAT
- Retain customer loyalty
- Accelerate solution and technology acceptance
- Create additional revenue streams
- Well-defined and reusable Operational Support System Framework
- ITIL-based Service Delivery
- 24X7 services to end customers
Case Studies
F100 Telecom Equipment Manufacturer Leveraged HCL's Effective Proactive Management and High End Technical Support for Competitive Differentiation
A smartly designed Co-Sourcing strategy can help product companies gain market share, accelerate new technology acceptance and achieve operational excellence. A Fortune 100 Telecom Equipment Manufacturer enabled new revenue streams, reduced their support services operational cost by driving operational efficiencies through processes and automation and achieved faster speed to market with multidimensional sourcing strategy by launching new products wrapped with Managed Services. Read more
World Leader in Mobile VAS Achieved Global Reach and Competitive Professional Services through HCL's unique Global Deployment Services
Similarly, a leading VAS provider achieved global reach and cost rationalization through right-shoring delivery approach, effective skill and resource management, leveraging HCL Global Delivery Centers, standardization of process and tools based effective program management. Read more
Africa's Largest Communication Service Provider Chooses HCL for IP RAN Roll Out; Achieves Speed-to-Market
Moreover, a leading Telecom Service Provider in Africa selected HCL as sourcing partner to implement IP RAN for mobile operations. HCL helped the customer to build Transmission/RAN network by designing, building, deploying and operating Cisco / Huawei based solution. The environment consisted of devices such as Cisco 7609, MWR2941 etc. HCL also helped the customer in building overall migration strategy including planning, verification and execution with operational support. Read more


text:
Creating cool text layers with smart objects in Photoshop
One advantage that certain vector based programs like illustrator has always had over Photoshop is the ability to do a lot of cool stuff with text. I have dabbled in Illustrator over the years but really am a rookie with this application and always prefer to use Photoshop since i've used it forever and it's quick and easy for me. With the default text tool you can create text in a vertical or horizontal orientation but did you know you can also create text and warp it in any custom fashion?. In today's tutorial i'll walk through a quick tip on how to convert text into a smart object so you can tweak it any way you need to.
I'll be using Photoshop CS6 for this demo and although it's not the perfect example show you how to put some curved text into the sky on this image. Where this does come in handy for me in real life is for wedding albums where I want to add some text into part of an image and a straight line just won't work.
When you create a text layer you always have the ability to go into the Edit –> Transform –> Warp menu item to do some preset edits to your text like Arc, Arch, Flag and many more. However many times you will want to have a custom shape to the text. To do this start with creating a normal text layer in Photoshop.
Next right click on the text layer and select Convert to smart object. This will allow us to create a custom transform for the text layer along with a few other nice benefits. After you convert this into a smart object you can go to Edit –> Transform –> Warp and select custom from the warp settings.
This will allow you to fully customize the warp of the text by dragging the end points around. You can even create a cool 3d style wrapping feel to it by moving one of the corners down so the text appears to be peeling like its coming off the page.
And since this is a smart object just double click on the text layer to open up the text in a new window. Edit the text in any way and save. Viola your text is updated and still keeps the custom shape that you created!
Really quick, easy and gives you full control over the shape of your text in Photoshop! Check back tomorrow for another really fun tip with customizing text in Photoshop. Also if you ever have a question on Photoshop, Lightroom or anything photography related just leave a comment below and lets chat.
If you like our articles remember to share them on Facebook, Twitter or +1 them on Google. I appreciate you spreading the word to your photo friends! Also remember to always start a conversation by leaving a Facebook comment at the bottom of the page. I love to hear from you.


text:
1-866-777-1360M-F 6am - 4:30pm PST
- Home
- Signs
- Wall Signs
- Danger - Arc Flash Hazard - Wall Sign
Most orders ship the same day. More | Click here to pay with a purchase order
For more information on this product, call us at 1-866-777-1360
Product Description
- Mounted on a durable 3mm PVC signboard
- Meets 2016 ANSI sign standards
- Industrial, long-lasting materials
- Custom signs available, please call 1-866-777-1360
- No set-up charge & no extra charges for customization
Danger - Arc Flash Hazard - Wall Sign
Post this sign where arc flash is a hazard in your workplace. Arc flash accidents can be much worse than shock hazards. The heat generated by an arc flash can melt or burn clothing from several feet away. The temperature of the arc flash can be as much as 4 times hotter than the surface of the sun. The blast from an arc flash can instantly damage hearing. These are all good reasons to have adequare signs wherever arc flash is a potential hazard.
This sign has several different mounting options to fit the needs of your location. It is made of durable vinyl that can survive in an industrial environment.
Warranty Information
At Creative Safety Supply, we guarantee the quality of our products. If you're unsatisfied with your purchase, you can return new, unopened items within 30 days of purchase for a full refund. That way you can feel confident the products you order will meet your expectations, or you'll get your money back.
For full warranty information, please visit our warranties page.
Product Reviews


text:
Emerson Automation Solutions has introduced the Roxar MPFM 2600 M multiphase flow meter. The Roxar MPFM 2600 M is a flexible and easily manageable wellhead measurement solution based on a field-proven technology platform. The meter meets the needs of many challenging applications and can be a cost effective solution for more constrained budgets. The meter is ideal for customers with direct and continuous wellhead multiphase flow monitoring needs.
The Roxar MPFM 2600 M is part of the scalable Roxar multiphase product family and provides flexibility as fields mature and conditions change. The compact meter can be easily retrofitted in the field, delivers straightforward installation and commissioning, and has been specifically designed to meet operators' capital expenditure and varying field requirements.
"The MPFM 2600 M is designed to help operators manage costs and increase efficiency while enhancing production and making smaller fields more viable," says Patrick Babka, vice president and general manager, Roxar, Emerson Automation Solutions. "It is also ideal for applications requiring one multiphase meter per well, allowing operators to track multiphase flow from all of their individual wells accurately and cost-effectively."
The MPFM 2600 M can identify and measure non-symmetrical flow in varying flow regimes, providing improved measurement uncertainty monitoring and reliability. The meter includes the advanced signal processing, field electronics and electrode geometry innovations of the third generation MPFM 2600.
Different modules can be placed into a variety of configurations and software modules are available to help with challenging applications including flow back measurements, well testing and allocation metering. These customised configurations include a venturi which extends the operating range of gas void fractions (GVF) to 100% (MPFM 2600 MV), a compact gamma system for improved accuracy (MPFM 2600 MVG), a formation water salinity measurement system, and a special operating mode for wet gas wells.
Emerson Automation Solutions
Please be civil. Job and promotion will not be added into the comment page.
Leave this field blank
Your name*
Your e-mail*
Your website
Leave this field blank
Your comment*
(Use Markdown for formatting.)
This question helps prevent spam:
What color is the sky on a clear day?*
Remember me
your browser.
Schlumberger has announced an agreement to acquire a majority (51%) equity interest in Eurasia Drilling Company Limited.
Mobile news
Our news
your website
Do you have any
tips to us
About us | Contact | Privacy | Terms |
Editor: Michael Rogers
Copyright 1973-2016. Scandinavian Oil-Gas Online. ISSN 1500-709X.
Tel: +47 22 44 72 70, Fax: +47 22 44 72 87


text:
866 E ROCKWELL Drive Chandler, AZ 85225
Hi, David Tucker, ABR, GRI, CDPE.
My name is
and I have a question about 866 E ROCKWELL Drive Chandler
You can respond by either emailing me at
or call during
My Question Is:
Estimated Monthly Payment$5,051
Directions South on McQueenWest on HighlandSouth on SabaWest on Rockwell to home on North side of Rockwell, Super charming 3 bedroom home in Chandler with a pool for these hot summer days! The open floor plan boasts toasted chestnut wood plank like floors and carpet in all the right places. A/C and roof are only 5 years young! Great location situated almost equally between downtown Chandler and downtown Gilbert. Across from the community pool and park! On the nearest major intersection, Warner and McQueen, sits the sought after North Chandler Legacy Traditional School campus. According to their website, ''Legacy Traditional School is an "A" Rated school district by the Arizona Department of Education and has been voted the #1 charter school in Arizona by Ranking Arizona''. Come see this home today!
Interior Features Include:
Fireplace Description 1 Fireplace
Kitchen Dishwasher, Built-in Microwave, Range/Oven Elec
Window Cov: Vinyl Frame, Dual Pane
Heating: Electric Heat
Floor Type: Carpet, Tile, Laminate
Exterior Features Include:
Covered Patio(s), Patio
Fence Block
Acreage: 0.1300 Acres
Landscape Desert Back, Desert Front
Square Feet 5628
Roof: Comp Shingle
Foundation Brick, Frame - Wood
Garage Capacity: 2
Other Features Include:
Subdivision: Stonegate Crossing
Water: City Water
Sewer: Sewer - Public
Re/max Fine Properties
Cell: (602) 762-7653
Fax: (480) 355-1714
Email: email@example.com
Information provided by the Arizona Regional Multiple Listing Service, and is deemed reliable but not guaranteed accurate. Copyright 2009 Arizona Regional Multiple Listing Service, Inc. All rights reserved. Indicates a property listed by a real estate brokerage other than the owner of this site.


text:
You didn't think that was all the Sex and the City style from season three did you? Pshht. Season three was not only one of the most turbulent of seasons, it was also one of the most fashionable. Did anyone else notice Carrie always dressed a bit more sophisticated for Big than Aidan? She was more casual and funky for Aidan. I like both styles for both men. One thing I really want to find before I die: Carrie's purple sequin Fendi baguette. Yes, the one that was stolen. Are you out there my pretty?
Join The Conversation
I'm not a fan of Samantha's look in this picture.
I always thought the shoes were amazing! I also like Miranda's dress.
I love this look on Carrie. The shoes.. So cool!
Love the shoes! hahah the colors are cool!
Like us on Facebook
Related Posts


text:
|Doji Ryobu
|Un-named daughter,
un-named son
|Emerald Champion,
Crane Clan Champion
Doji Ryobu was the Crane Clan Champion [1] and Emerald Champion. [2] during the 9th century.
Emerald Champion Edit
In 811 Ryobu, the Crane Clan Champion, unexpectedly won the tournament of the Emerald Champion. Emperor Hantei XXII announced his son would marry Ryobu's sister, allowing the Crane maintain a strong position in a political climate dominated by the Scorpion and Lion. [3]
Return of the Ki-Rin Edit
In 815 a force of tainted barbarians had invaded Rokugan crossing at the northern edge of the Kaiu Wall. During the final month of the Imperial Winter Court at Otosan Uchi, appeared Shinjo Nishijin, bearing official travel papers endorsed by the Master of Air. The Unicorn Clan Champion told about the Return of the Ki-Rin, and produced the sandalwood fan. Ryobu welcomed the descendants of the Ki-Rin Clan [4] when he received the fan sent by the Unicorn Clan upon their Return. [5] In return for this recognition Shinjo Nishijin gifted Ryobu with a small herd of horses. They were from the Kami Shinjo stock, stronger, larger, and smarter than any before seen in the Empire. The strongest of them was named Junko. [6]
|Preceded by:
|Crane Clan Champion
(c. 811)
|Succeeded by:
Doji Ryobu's son
|Preceded by:
|Emerald Champion
811 - ?
|Succeeded by:
- ↑ Way of the Crane, p. 108
- ↑ Way of the Unicorn, p. 39 sidebar
- ↑ Imperial Histories 2, p. 155
- ↑ Imperial Histories 2, pp. 156-157
- ↑ Way of the Crane, p. 119
- ↑ Scenes from the Empire 18, by Nancy Sauer, Robert Denton, Yoon Ha Lee, and Shawn Carman
|This Crane Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
https://folkarttowers.siparislerim.com/login web adresinden de sisteme giriş yapabilirsiniz.
Sistem hakkında daha detaylı bilgi için www.siparislerim.com adresini ziyaret ediniz.Folkart Concierge application, Folkart Towers office workers and to facilitate the lives of the residents of residential services and products with a single tap brings you to your feet. Online membership is free, and membership information for the user name and password management Folkart Towers your request.
https://folkarttowers.siparislerim.com/log can log into the system through the web site.
For more information about the system, please visit us at www.siparislerim.co.


text:
Latest Photos
Events/Live Bands
Mobile Marketing Forms
Copyright EclipsePenarth© 2014. All Rights Reserved.
Stylish and contemporary the bar offers the perfect place to meet, drink and enjoy the company of like minded party people
of Penarth.
The second floor is our nightclub with a resident DJ, great light and sound system playing the best in R&B, Hip-Hop and club classics we open until late.
The core principles of Eclipse are that you should have a fun, relaxed and pleasurable nights out. So our staff are
on hand to ensure you are looked after. Let us look after you and make sure your night is unforgettable and magical in
every way. We hope to see you soon.
Keep up to date with our events, Plus be entered in our monthly Prize draw a great prize every month. Sign up for Email Alerts
Book your Party now, Birthday, Hen Night, Engagement what ever the occasion we can accomodate!
Click Here
If your holding a charity event and need a venue get in touch we would be happy to help.
Studiofrog Web Design


text:
No Tickets Left?
Provide Your Contact Information Below
01/22/14 7:00pm – George Mason Patriots at Fordham Rams Basketball
See All Fordham Rams Basketball Events
Rose Hill Gym
January 22, 2014, Wednesday @ 7:00pm
With millions of fans nationwide, Ticket Monster
is your destination for exclusive discounts on
sports, concerts, and theater tickets.
Sign up and never miss a deal!
Enter your email address and we will send you an email with instructions on how to reset your password.


text:
In Stock
Expected to ship by .
Rollover to zoom
Click to enlarge
To view an item in an alternate color, place your mouse over a swatch above.
To view item in an alternate color, click on a swatch above.
Item #: 1165-24034-1252
This ultra-comfortable men's big and tall track jacket by KS Sport™ is the perfect casual jacket for wherever life takes you. Made with you in mind, we've designed this jacket in the most generous, comfortable fit imaginable. You'll also love its moisture-wicking technology that absorbs sweat and keeps you cool, and vibrant contrasting colors.
Men's big & tall track jacket in sizes: Big: XL, 2XL, 3XL, 4XL, 5XL, 6XL, 7XL, 8XLTall: L, XL, 2XL, 3XL, 4XL, 5XLBig length abt. 32"Tall length abt. 34"
Our Expert Opinion Made for the field, but perfect for any casual lifestyle! Pair this great track jacket with any of our extra-wide athletic shoes or any of our moisture-wicking sweatpants by KS Sport™ for to experience the ultimate in athletic leisure.
To help you achieve the best fit possible, we've provided a few tips for you to use as a guide
when taking your body measurements. We have also provided a size chart to assist you when selecting merchandise.
You should order Tall if you are 6' 2" or taller and your waist measures less than your chest.
You should order Big if you are 6' 2" or shorter and your waist measures the same or larger than your chest.
(based on 4 reviews)
of respondents would recommend this to a friend.
Ratings Distribution
5 Stars
4 Stars
3 Stars
2 Stars
1 Stars
Best Uses
Feels too small
Feels true to size
100% (3 reviews)
(3 reviews)
Feels too large
Reviewed by 4 customers
Displaying reviews 1-4
Back to top
Bought two will go back for more
By Kevin
from WI
About Me Casual Dresser
Comments about KingSize 1/4-Zip Pullover Track Jacket by KS Sport:
Bought two of these and I wear them all of the time now that the weather is getting nicer...perfect for the transition from winter to spring and spring to fall
Bottom Line Yes, I would recommend this to a friend
Was this review helpful? Yes / No
- You may also flag this review
Nice light warm jacket for early spring up north also.
By BroadwayJoe
from Florida
Coaching, makes a nice light over layer
i would buy the product again
By huff
from brooklyn ny
fit perfect like color and style
Represents itself as a cheap pajama top only. No quality
By Big Gene
from Federal Way, WA
About Me Gym Rat
Poor quality, color-block design is way off
Bottom Line No, I would not recommend this to a friend
Be the first to know about new products & special offers and receive a 30% OFF COUPON in your inbox
Get to know us
Privacy Policy |
Terms of Use |
California Privacy Rights |
California Transparency in Supply Chains Act.
© 2017 FULLBEAUTY Brands, L.P. All Rights Reserved.
KingSize® is a registered trademark of FULLBEAUTY Brands, L.P.


text:
I'm talking about the counter-culture that thinks "The Dark Knight" sucks and believes "Batman & Robin" to be an unsung, comedy gem.
I'm also kind, caring and genuinely interested in getting to know you, should we click!
I enjoy walking, writing, movies, video games, and lengthy, in-depth discussions such as "would Superman or Goku win in a fight?" (hint: Superman.)
*Disclaimer: I'm way more normal than what this roller-coaster ride of a profile will lead you to believe. So don't fear about my ability to stay calm and classy and restrain my urge to argue with people who say things like "Super Mario Land is the worst Mario game."
I've had two fright-filled seasons as a Zombie SWAT Officer at "Terror Behind the Walls," and you can currently see my smiling face in "How to Get Away with Murder," "Billions," "Master of None," "Quantico" and, it's-pretty-much-Rocky-7, "Creed."
I've also been working on an overly complicated board game (think Risk meets Monopoly but in space) and a short film!
I also have this weird knack for creating characters, stories and worlds, but then fail to actually make them a reality. So that means plenty of production bibles, but no production.
I'm also a champion cuddler.
There's also probably the "why is this rascal dressed as a Star Sheriff?"
A reasonable question.
As for modern television, "Designated Survivor," "American Ninja Warrior," "This is Us," "One Punch Man," and "Gilmore Girls." Yes, even much of the wonky new season.
I'm actively infuriated by crime dramas and their lack of real overarching plots, and avoid things everyone loves, like "Game of Thrones" and especially "The Walking Dead."
As for films, I was a film major, so my taste is eclectic at best. "Star Wars," Kurosawa, "The Last Starfighter," etc.
I'm a very slow reader, but I'm currently in the middle of two "Star Wars" novels, "Cosmos" by Carl Sagan, "The Name of the Wind," and a collection of Washington Irving stories. I also enjoy DC graphic novels and comics (I become less and less enamored by Marvel with each passing minute.)
With music, I prefer classical, or film and video game soundtracks, although I'm not adverse to music with lyrics, such as "The Killers" or 80s hair metal.
This is a stupid section.
I know it's gotta be something creative, but I can't yet pinpoint it!
Perhaps you can change up my routine by going on a date with me? ^_^
I frequently match my socks, shirt and boxers, although my boxers tend to be ironically related to the shirt I'm wearing.
I made a FetLife profile (for whatever reason) and then erased it hours later.
But otherwise, if anything on here has caught your attention, feel free to let me know.
I really like meeting people who are different than me because I love to learn about things I'm unfamiliar with (and I'd hope that you do, too!) So even if it doesn't seem like we'd be a good match on the surface, don't let that stop you from messaging. You might be surprised!


text:
Destruction Unit - Night Loner | Newtown Radio | Swan 7 Studios MP3
http://dunit.ascetism.com/ http://www.newtownradio.com http://www.swan7.com Filmed and recorded at Swan7 Studios Curated for Newtown Radio by Colin ...
Saturday Night Loner Dance - Awesome Vines! MP3
Make sure you subscribe to Awesome Vines! to be updated with the most recent creative/comedic vines! Vine by: Kc James I do NOT own this Vine nor do I take ...
CNBLUE [Blue Night concert] - I'm A Loner MP3
CNBLUE [Blue Night concert] - I'm A Loner ☆º°˚*
Three Dog Night - The Loner MP3
Kid Cudi - Day 'N' Nite MP3
Music video by Kid Cudi performing Day 'N' Nite. (C) 2009 Universal Motown Records, a division of UMG Recordings, Inc.
Day 'n' Nite (Nightmare)
Kid CudiDay and night (what, what) I toss and turn, I keep stressin' my mind, mind (what, what) I look for peace, but see I don't attain (what, what) What I need for keeps, this silly game we play, play Now look at this (what, what) Madness, the magnet keeps attracting me, me (what, what) I try to run, but see I'm not that fast (what, what) I think I'm first but surely finish last, last [...]
Destruction Unit - Night Loner (unofficial video experiment) MP3
These guys are awesome.
Night Loner MP3
Provided to YouTube by BWSCD, Inc. Night Loner · Destruction Unit Deep Trip ℗ 2013 Destruction Unit Released on: 2013-08-20 Auto-generated by YouTube.
The Loner MP3
Provided to YouTube by Universal Music Group North America The Loner · Three Dog Night One ℗ 1968 Geffen Records Author, Composer: Neil Young ...
Night Loner-Demo One MP3
Track Listing 0:00 - Sexually Frustrated 1:36 - Thespian 2:57 - Pipe Dreams 3:57 - Kill Myself 5:11 - Big Doings (Corpsicles) Download at ...
Loner: Kali Uchis / Nick Knight / Younji Ku MP3
http://showstudio.com/project/loner After discovering her soulful music on Instagram, Nick Knight quickly made Colombian singer/songwriter Kali Uchis' music ...
Destruction Unit - "Night Loner" - 1/10/14 Saint Vitus Bar, Brooklyn MP3
Man-Aye - Mr. Loner In The Night [HQ] MP3
Music by Man-Aye performing "Mr Loner In The Night" .© 2014 DreamOn Entertainment Man-Aye Instagram @Man_Aye Man-Aye Personal Facebook ...
Kid Cudi - Day n Night Lyrics ! (Original) MP3
Kid Cudi - Day n Night Lyrics ! (Original) ╔═╦╗╔╦╗╔═╦═╦╦╦╦╗╔═╗ ║╚╣║║║╚╣╚╣╔╣╔╣║╚╣═╣...
Saturday Night Loner's Club Ep. 1 MP3
New series I've created, and I hope to actually post it on Saturday from now on. This episode includes Wrecking Journals, a vintage makeup tutorial, and the ...
Saturday Night Loner's Club Ep 2 MP3
Hello again! Here's the second episode of Saturday Night Loner's Club! This episode includes tongue twisters, a cooking tutorial, and some jams(music not jelly) ...
Saturday night loner dance. MP3
Me and my sis made this cool vid all by ourselves :)
Saturday night loner dance again MP3
Mischief Night - Lucci Loner Feat. Briana Devoue MP3
Download Now on Datpiff.com! Lucci Loner - G.O.M.L. Presented by Billy Danze (M.O.P.)
Zedd - Stay the night remake on LMMS by Shown Loner MP3
Sean Wallace & One Night Stand - 'Loner'. MP3
Sean Wallace performing a new song 'Loner' with One Night Stand @ The Working Men's College, Camden, London. - June 2010. Sean Wallace's band The ...
Appalachian Trail Loner #95 NIGHT OF THE OWLS 2012 Thru Hike MP3
(0:04) Several roads were still washed out, damaged by Hurricane Irene. (0:11) The Stratton mountain caretaker cabin has been here since 1928. It has a brand ...
Loner On Saturday Night Lmao MP3
This video was uploaded from an Android phone.
Saturday night loner dance | 1 HOUR ORIGINAL LOOP MP3
Like it if you want to be updated on my new projects and subscribe to my channel too ;) Original maker of the video on Vimeo is Kc James, this is the 1 hour loop ...
Saturday night loner dance MP3
Loner dance.
Man-Aye - Loner In The Night (Music Video) MP3
Man-Aye Facebook http://tinyurl.com/cxc5p2m Man-Aye Personal Facebook http://tinyurl.com/cfp4kfj Man-Aye Twitter http://tinyurl.com/cvhzner DreamOn Ent.
Scary Loner - This Night is My Daughter (Junk Hop Remix) MP3
Rocksanna-Loner of the night MP3
Rocksanna playing song loner of the night at Phoenix pub 19.12.2010 Lučenec Rony Janeček Kika Hukkelová Aďo Janeček Marek Jamber.


text:
Forage to Fork: Spring Cheese
Whole Foods Market Dedham Culinary Center
Tuesday, May 20, 2014 from 7:00 PM to 8:00 PM (EDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
The menu will be determined closer to the date of the class, due to seasonality of the various cheeses.
While cheese doesn't normally come to mind when thinking of seasonal ingredients, it actually is! Because milk production typically ramps up in the spring when mothers are making milk to feed their young, many sheep and goat cheeses peak at this time of the year, specifically young cheeses that don't require a great period of aging to mature. We'll show you how to use the best of these cheeses in tasty, creative recipes. This class is part of our Cooking Live series.
About Cooking Live: Turn off the TV—we know you're watching cooking shows anyway—and let our Culinary Center chef guide you through new recipes and techniques. These classes will feel like watching your favorite chef on TV, except we're here to answer all of your culinary questions. The best part? You won't leave feeling even hungrier than when you walked in!
When & Where
Whole Foods Market - Dedham
300 Legacy Pl
Tuesday, May 20, 2014 from 7:00 PM to 8:00 PM (EDT)
Add to my calendar
Whole Foods Market Dedham Culinary Center
Founded in 1980 in Austin, Texas, Whole Foods Market (wholefoodsmarket.com, NASDAQ: WFM), is the leading natural and organic food retailer. As America's first national certified organic grocer, Whole Foods Market was named "America's Healthiest Grocery Store" by Health magazine. The company's motto, "Whole Foods, Whole People, Whole Planet"™ captures its mission to ensure customer satisfaction and health, Team Member excellence and happiness, enhanced shareholder value, community support and environmental improvement. Thanks to the company's more than 73,000 Team Members, Whole Foods Market has been ranked as one of the "100 Best Companies to Work For" in America by FORTUNE magazine for 15 consecutive years. In fiscal year 2012, the company had sales of $11.7 billion and currently has more than 340 stores in the United States, Canada and the United Kingdom.


text:
my name is jonas by Reza-ilyasaWatch
©2007-2017 Reza-ilyasa
unfinish, but mehh, im in rush... thankyou for viewing..
hey that's my name
Great work!
Black white and one red. Levels out the the overall picture.
lol My name is jonas ^^ you wouldn't mind if I use it as my facebook profile photo?
...and I'm an alcoholic.
this is really nice I love the pose very anime very stylish, where is this character from?
Nice and simple, although I think the tree interferes with the composition somehow; it melds in to the handle of his blade. What do you think?
je m apelle jonas aussi
kesan doodlenya malah bikin keren...
ugh ajarinnn...
ugh ajarinnn...
Wuidih..cool berat!
wah...temennya kopral jhono yah?
playing guitar hero? and weezer? this rawks
slesaiyn doong bang! .. keren gila niy..
keren !!
"I'm carrying the whale!"
heh tense
heh tense
ow ow
really really good, i love the positioning of the camera and its tilted effect. ^^
Very good


text:
The city is adding three Gore district properties to a registry that would buy a little more time should anyone ever apply to demolish them.
All of the properties in the district that are listed in the city's heritage inventory, minus 24 and 28 King St. E., will be added to the downtown heritage register. That means should anyone apply to demolish them, there will be a wait time of 60 days after a permit is issued for council and the public to bring forward concerns. Currently, it's only 20 days.
"The net impact is another 40 days for us to have the opportunity to bring something forward if it's serious enough," said Tim McCabe, general manager of economic development.
The city issued a demolition permit to developer Wilson Blanchard for 24 and 28 King St. E. earlier this year to build a new development there, which sparked some outcry. That inspired Coun. Jason Farr of Ward 2 downtown to introduce the motion at the general issues committee on Monday.
The move bridges the gap between the properties being provincially designated — which would mean no demolition at all — and the usual 20-day period for other city properties, Farr said.
"It would allow for the kind of consultation that was obviously seen, albeit in the final hour here" on the Blanchard development, Farr said.
There is still opposition to the demolition of 24 and 28 King St. E., which were built in the 1870s. But Farr said he has faith in what will replace it.
"This is difficult nonetheless," he said. "I'm not going to sugar coat it. It's a tough decision. But I've said many times that bringing more people to the core is going to help us in the end."


text: Ferrari Is California Dreamin' A new Ferrari for a new generation. By Alessandra Ilari on November 24, 2008 A new Ferrari for a new generation. It's not exactly Ferrari Lite, but the fabled Italian sports car brand's latest model, the California, is just in time for the pinched economy. For one thing, it's more understated, smaller and lighter than the company's other testosterone-fueled beasts, even if it can go from 0 to 60 mph in less than four seconds. And, if past Ferraris focused more on rpm, torque and horsepower, the California takes comfort equally into account, from its retro feeling, based on the brand's 1957 California 250, to a roomier interior with handcrafted leather seats and a sophisticated gearbox with automatic or manual options. WWD logo To Read the Full Article SUBSCRIBE NOW Already a Subscriber? Log in. Tap into our Global Network Of Industry Leaders and Designers load comments blog comments powered by Disqus


text:
Read the full story here Web Link posted Saturday, August 4, 2012, 8:59 AM
Town Square
Feature story: A measure of his heart
Original post made on Aug 4, 2012
Read the full story here Web Link posted Saturday, August 4, 2012, 8:59 AM
Comments (12)
a resident of Downtown North
on Aug 4, 2012 at 11:22 am
Lawrence is a man with unmatched courage. He is just one amazing fellow and we are fortunate to call him a Palo Alto citizen. GO Lawrence! ==
a resident of Mountain View
on Aug 4, 2012 at 12:41 pm
this shows some humanity in someone from palo alto. too bad there are so many heartless individuals. go to your synagogue or church to find people who may actually display that they have heart
a resident of Green Acres
on Aug 4, 2012 at 11:09 pm
Amazing individual. Thanks for the story. Very impressive story that got me motivated. I am so proud to live in the same city as this hero!!
a resident of Barron Park
on Aug 6, 2012 at 10:26 am
heart warming and encouraging. thank you
a resident of University South
on Aug 6, 2012 at 5:11 pm
This is wonderful. I have loved Lawrence for years - so, how do we help him to raise money?
a resident of Community Center
on Aug 6, 2012 at 8:39 pm
Thank you for telling your story Lawrence! Unmatched courage is right, you are an inspiration! The article didn't say anything about your wonderful sense of humor! Go Lawrence!!
a resident of Midtown
on Aug 6, 2012 at 11:31 pm
What a fantastic story!
a resident of another community
on Aug 15, 2012 at 5:41 pm
Hi Larry--
So glad to hear you are still up to great things!!
a resident of Barron Park
on Feb 1, 2013 at 6:23 am
What a great guy and friends. I can't believe he's gone.
a resident of Professorville
on Feb 1, 2013 at 5:19 pm
I grew up in South Palo Alto as a best friend to Larry's sister Janice. My heart is broken that such a wonderful human being has lost his fight. It's especially hard for those of us who have ourselves received the dreaded news that we have "cancer." I will comfort his sister and believe he is now an angel.
a resident of Charleston Meadows
on Jul 18, 2014 at 9:30 am
Best big brother in the whole wide world.... I will FOREVER miss this angel on earth that has now moved on.... I love you, Larry...
a resident of Greenmeadow
on Apr 17, 2015 at 10:37 pm
I will FOREVER love and miss Uncle Larry. He was an amazing, caring, heroic man.
Don't miss out on the discussion!
Sign up to be notified of new comments on this topic.
Opening alert: Kyosho Sushi in Menlo Park
By Elena Kadvany | 0 comments | 1,821 views
Love is a Verb
By Chandrama Anderson | 0 comments | 1,128 views
Oh, My Aching Knees
By Max Greenberg | 15 comments | 802 views
Save $5 when you register by Monday, July 24
Registration is now open for the 33rd annual Palo Alto Weekly Moonlight Run and Walk. This family-friendly event which benefits local nonprofits serving kids and families will take place on Friday, Oct. 6 at the Palo Alto Baylands.


text:
Dropped the ball on this series as of late, but what better way to spark it up again than with the guy people would rather watch push? Save one seminal ledge skating part, Gino's picks were completely unexpected, transition-heavy and refreshing. He even called back a minute after we got off the phone to say he couldn't just list five and had to add one in (it's the one from 1997 by the way), hence the asterisk next to the five.
Always open to requests for Five Faves, and thanks for bearing with us as we begin to pull the Quartersnacks Editorial Department off the Fall-Off List ;) ♥
Related: An Interview With Gino Iannucci (2012)


text:
Three-seat hammered iron Bishop's altar bench with red crushed velvet and decorative cross and scrolls.
73" wide x 24" deep x 58" tall
This altar bench is also sometimes called a sedilia. Some older examples of sedilias were often set back into the main wall of the church. These were placed at the front of the church, so that the front altar could be accessible for the users of the bench. These included the priest, who sat in the middle and largest chair, the deacon, and the sub-deacon. The seats were covered with green on ordinary days of the liturgical calendar, and purple during the holiday seasons.


text:
Your location is set for ASHBURN, VA 20146
Select a product to get a quote.
Edit location
Retrieve a saved quote
Log in to My Account
Agents near 20146
Edit location
24-hour support
Login to My Account to easily print your insurance cards. Not registered for My Account? No problem. Print without logging in by entering your policy number, last name and ZIP code.
Download the Allstate® Mobile app to view and save a copy of your insurance card to your smartphone.
Download for iOS
Download for Android
Please select your state from the dropdown to view additional information regarding digital ID cards.
Contact your Allstate Agent or call 1-800-255-7828. We're here for you.
1Self-print option is not available in California, Delaware, Hawaii, New Jersey, New York and Vermont.
2Not all states accept digital insurance cards as proof of insurance. Message & data rates may apply. Text HELP for help. Privacy: Allstate.com/privacy
20146 Edit Location
© 2017 Allstate Insurance Company
ZIP Code
City, State


text:
The Stranger's Inn was an inn located on the outskirts of the Merchant District of the Second City. Newcomers easily found it when entering the city from the river and major road to the west. The maximum period to stay was one week and no one who had stayed in the Inn before was ever allowed to stay again. In 1198 it was run by Shinjo Xiang. [1]
- ↑ Second City - The City, p. 55
|This Unicorn Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
Confidentiality or Continuity? Family Caregivers' Experiences With Care for HIV/AIDS Patients in Home-Based Care in Lesotho
Makoae, Mokhantso G.
Jubber, Ken
Bibliographic Citation
SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance / SAHARA , Human Sciences Research Council 2008 April; 5(1): 36-46
MetadataShow full item record
Related items
Showing items related by title, author, creator and subject.
Quality of Life of Cancer Patients Receiving Inpatient and Home-Based Palliative Care Peters, Louise; Sellick, Ken (2006-03)
Advanced Home Care for Cancer Patients at the End of Life: A Qualitative Study of Hopes and Expectations of Family Caregivers Wennman-Larsen, Agneta; Tishelman, Carol (2002-09)
Home-Based Long-Term Care: An Interview With Miriam Hirschfeld Davis, Anne J.; Hirschfeld, Miriam (2002-01)


text:
Don't look away for even a second.
One clear summer day in a Baltimore suburb, a baby goes missing from her front porch. Two young girls serve seven years for the crime and are released into a town that hasn't fully forgiven or forgotten. Soon, another child is missing, and two detectives are called in to investigate the mystery in a community where everyone seems to have a secret.
This is Every Secret Thing and you are watching MovieZion
Please wait for 3 seconds, we are loading Every Secret Thing stream.
Please enter captcha to continue to video.
If the above stream is offline (saying We are sorry!),
please click on the "Refresh Link" to get a new streaming link. Don't worry it'll just take a second.