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Hypoxia-mimetic agents inhibit proliferation and alter the morphology of human umbilical cord-derived mesenchymal stem cells
The therapeutic efficacy of human mesenchymal stem cells (hMSCs) for the treatment of hypoxic-ischemic diseases is closely related to level of hypoxia in the damaged tissues. To elucidate the potential therapeutic applications and limitations of hMSCs derived from human umbilical cords, the effects of hypoxia on the morphology and proliferation of hMSCs were analyzed.
After treatment with DFO and CoCl2, hMSCs were elongated, and adjacent cells were no longer in close contact. In addition, vacuole-like structures were observed within the cytoplasm; the rough endoplasmic reticulum expanded, and expanded ridges were observed in mitochondria. In addition, DFO and CoCl2 treatments for 48 h significantly inhibited hMSCs proliferation in a concentration-dependent manner (
The hypoxia-mimetic agents, DFO and CoCl2, alter umbilical cord-derived hMSCs morphology and inhibit their proliferation through influencing the cell cycle.
Human mesenchymal stem cells (hMSCs) were first identified by Friedenstein et al.
Oxygen is a potent signaling molecule, affecting the fundamental characteristics of various types of cells. Specifically, reduced oxygen levels or hypoxia influences blood-brain barrier permeability through influencing endothelial cell junctional complexes
Hypoxia is often induced by decreasing oxygen concentrations
Analysis of stem cell proliferation and differentiation is often carried out in 20% O2, which is much greater than that found
Morphological and immunophenotypic characterization of umbilical cord-derived hMSCs
Both large and occasionally multi-nucleated hMSCs as well as small, spindle-shaped mononucleated cells were present in the primary hMSCs culture (Figure
Figure 1
Morphological changes in hMSCs primary cultures over time
Morphological changes in hMSCs primary cultures over time. A) First passage at 5 days (100×). B) Third passage at 3 days (50×).
The primary hMSCs expressed CD44 (96.1%), CD29 (98.5%), and CD105 (98.6%) surface antigens whereas expression of the CD106 (2.1%), CD40 (0.8%), CD34 (0.5%), CD45 (0.8%), and HLA-DR (0.7%) surface molecules were below the detection limit (Figure
Figure 2
Histograms representing the immunophenotype and cell cycle distribution as determined by flow cytometry
Histograms representing the immunophenotype and cell cycle distribution as determined by flow cytometry. The phenotype of hMSCs was determined after cell isolation (A). The cell cycle distribution was determined without (control) or with 120 μM DFO or with 100 μM CoCl2 for 48 h (B).
hMSCs differentiation
Following adipogenic induction, hMSCs morphology changed from elongated, confluent fibroblastic-like cells (Figure
Figure 3
Adipogenic and osteogenic differentiation of hMSCs
Adipogenic and osteogenic differentiation of hMSCs. Adipogenesis was detected by the formation of intracytoplasmic lipid droplets stained with oil red O (A, no induction; B, induced cells; 100×). Osteogenic differentiation was demonstrated by calcium deposition as evidenced von Kossa staining (E, no induction; F, induced cells; 100×). Atomic force microscopy of umbilical cord-derived hMSCs upon adipogenic (C, D) and osteogenic (G, H) differentiation after 21 days in normoxic conditions were shown as error signal images (C,G) and 3-dimension graphs (D,H).
Effects of DFO and CoCl2 on hMSCs proliferation
hMSCs proliferation rates were determined using the MTT assay; logarithmic growth was observed after 48 h. Decreased hMSCs proliferation was detected after DFO (Figure
Figure 4
Reduced hMSCs growth after treatment with hypoxia mimetics
Reduced hMSCs growth after treatment with hypoxia mimetics. A) hMSCs were treated with the indicated concentration of A) DFO or B) CoCl2 at various time points. Data represent the mean ± SD. *
Effects of hypoxia mimetics on hMSCs cell cycle
As compared to the control group, the ratio of hMSCs in the G0/G1 phase increased while those in the G2/M/S phase decreased after DFO and CoCl2 treatment (Table
Table 1
Effects of DFO and CoCl2 of different concentrations on cell cycle distribution of hMSCs detected by flow cytometry with PI staining
DFO (μM)
CoCl2 (μM)
Cell phases
80.03 ± 4.28
82.67 ± 3.25
86.41 ± 3.13
81.96 ± 2.21
85.87 ± 2.93
1.52 ± 0.57
1.35 ± 1.29
1.30 ± 1.32
1.37 ± 0.29
1.32 ± 0.74
18.45 ± 4.10
15.98 ± 4.21
12.29 ± 1.95
16.67 ± 2.13
12.81 ± 2.87
(n = 3)
Effects of hypoxia mimetics on hMSCs morphology
After 72 h in normoxic conditions, AFM revealed enlarged, spindle-shaped hMSCs up to 80 μm in length and 30-40 μm wide (Figures
Figure 5
Atomic force microscopy of hMSCs under normoxic conditions
Atomic force microscopy of hMSCs under normoxic conditions. A typical long spindle, cytoskeleton (A1, A2, A3), and palpus-like or cicada wing-like pseudopodium (B1,B2, enlarged areas shown in C1 and C2 respectively) were observed. The mesh-like cytoskeleton of adjacent hMSCs (D1, D2) can also been seen (enlarged areas shown in E1, E2).
hMSCs were exposed to factors inducing adipogenic and osteogenic differentiation for 21 days in normoxic conditions after which the morphology was assessed. The nuclei of adipogenic-induced hMSCs moved to peripheral area while the cell membranes became more granular, which was suggestive of the S phase of the cell cylce (Figures
Altered hMSCs morphology was observed after treatment with DFO (Figures
Figure 6
Atomic force microscopy of hMSCs upon treatment with hypoxia mimetics
Atomic force microscopy of hMSCs upon treatment with hypoxia mimetics. hMSCs were treated with 120 μM DFO (A1, A2, A3) and 100 μM CoCl2 (B1, B2, B3) for 4 days. Enlarged areas of B1 and B2 are shown in C1 and C2 respectively.
TEM analysis of control hMSCs revealed round cells with surface protrusions and microvilli (Figure
Figure 7
Transmission electron microscopy of hMSCs
Transmission electron microscopy of hMSCs. A) Complete view of untreated (8900×) or D and E) DFO-treated hMSCs after 3 days (8900×). (B and C) The endoplasmic reticulum of untreated hMSCs (24000×). F) The mitochondria of untreated (30000×) and G) DFO-treated hMSCs (30000×).
Human umbilical cords represent a promising source of hMSCs, which can be conveniently isolated and induce low immunogenicity. In addition, as compared to other stem cell sources these present relatively few ethical issues. The hMSCs isolated from umbilical cords were positive for CD44, CD29 and CD105, and negative for CD106, CD40, CD34, CD45 and HLA-DR surface antigens, confirming their identity as MSCs
The intracellular cytoskeleton responds to external mechanical stimuli
Differentiation of MSCs is also influenced by hypoxia. Qu et al.
As evidenced by clinical trials in hypoxic-ischemic diseases, MSCs-based therapy has potential value in tissue replacement and regeneration
The influence of hypoxia on hMSCs ultrastructure was also explored by TEM. After treatment with DFO, hMSCs contained a large number of unidentifiable vacuoles that are early markers of apoptosis, which is consistent with Ren et al.
The effects of CoCl2 and DFO on hMSCs proliferation may be mediated by cell cycle changes. A larger percentage of DFO- and CoCl2-treated cells in the G0/G1 phase was observed, while the ratio of those in the G2/M/S phase decreased. Similar results were reported by Holzwarth et al.
There are several study limitations that warrant discussion. Firstly, the present study analyzed the effects of hypoxia mimics on hMSCs; however, the effects of physical hypoxia were not assessed. Further studies will be carried out to compare the effects of physical hypoxia and hypoxia mimetics on hMSCs morphology and growth. In addition, the effects of DFO and CoCl2 on hMSC HIF-1α levels were not analyzed. However, previous studies using the same concentrations of DFO and CoCl2 used in the present study have reported upregulation of HIF-1α expression
We isolated and cultured hMSCs from umbilical cords. The proliferation of hMSCs was inhibited by DFO and CoCl2. The cell surface and ultrastructure was viewed with atomic force microscopy and transmission electron microscopy after DFO- and CoCl2-induced hypoxia, which is the first time this has been reported. These data will provide a better understanding of the potential therapeutic applications of hMSCs in hypoxic-ischemic disease.
Isolation and culture of hMSCs from umbilical cord and treatment with hypoxia-mimetic agents
hMSCs were obtained from the umbilical cords of healthy full-term infants born by cesarean section. Tissues were washed with phosphate buffered saline (PBS), cut into approximately 1 cm3 pieces, and digested with 0.2% type II collagenase (Gibco, USA) at 37°C for 5-6 h. After filtration and centrifugation, the cells were incubated in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS, Gibco, USA), 100 U/mL penicillin and 100 U/mL streptomycin. Cells were inoculated at density of 1 × 106 cells/mL in 25 cm2 culture flasks (Corning, NY) and maintained at 37°C in a humidified atmosphere containing 5% CO2. After 4-5 days, non-adherent cells were removed. The culture medium was replaced every 3 days, and adherent cells were cultured until they reached 80-90% confluence. Cells at passage 3-7 were used to analyze the effects of hypoxia-mimetic agents. Informed consent for the use of the umbilical cords was obtained from each mother prior to surgery. The study was approved by The Ethics Committee of the First Affiliated Hospital, Jinan University, Guangzhou, China.
DFO and CoCl2 were dissolved in ultrapure water and applied to the hMSCs cultures at concentrations of 10, 25, 50, and 100 μM for CoCl2 and 15, 30, 60, and 120 μM for DFO. Control groups consisted of cells grown in the absence of DFO and CoCl2.
Immunophenotype determination and cell cycle analysis
hMSCs were incubated with either phycoerythrin or FITC-conjugated antibodies specific for CD29, CD44, CD90, CD105, CD106, CD40, CD34, and CD45 (BD Biosciences, San Jose, CA). Mouse isotype control antibodies served as negative controls. Cells were stained with a single label and analyzed by flow cytometry using a BD FACSCalibur.
Cell cycle was analyzed through propidium iodide (PI)-staining. hMSCs (106 cells) in the third passage were fixed, permeabilized in 70% cold ethanol at 4°C for 24 h, and treated with 200 μg/mL RNase A for 30 min at 37°C. hMSCs were then incubated in 40 μg/mL PI for 5 min at room temperature, and placed on ice until analysis by flow cytometry.
Analysis of hMSCs differentiation
To evaluate hMSCs properties, adherent cells (in the third passage, at 80-90% confluence) were subjected to adipogenic and osteogenic differentiation
MTT proliferation assay
After treatment, cell growth media was replaced with 200 μL MTT solution (5 g/L in PBS) per well for 4 h after which it was replaced with 150 μL dimethyl sulphoxide. Quantification was then carried out using a microplate reader at 570 nm; a 630 nm filter was used as a reference.
Atomic force microscopy
hMSCs were cultured on glass coverslips in growth media alone (control group) or containing 120 μM DFO or 100 μM CoCl2. After the cells reached 70-80% confluence at 72 h, they were gently washed with PBS, fixed with 4% paraformaldehyde, washed with demineralized water, and air dried. Tapping-mode atomic force microscopy analysis (Autoprobe CP research, Thermomicroscopes, California, USA) was performed with silicon nitride tips (spring constant, 0.9 N/m) using a Park Scientific Instruments commercial instrument (MPP-31123). The scanning speed was 0.4-1HZ. The IP2.1 software (Thermomicroscopes Proscan Image Processing Software Version 2.1, California, USA) applied smoothing analysis to the resulting images.
Transmission electron microscopy
TEM was used to assess the morphological characteristics of hMSCs. hMSCs cultured for 72 h in media containing 120 μM DFO were immediately fixed at 4°C with 2.5% glutaraldehyde, post-fixed in osmium, and routinely processed for TEM assessment (PHILIP5 TECNAI 10, HOLLAND). Cells cultured in complete medium alone were used as a control group.
Statistical analysis
Statistical analyses were performed using SPSS 15.0 statistics software (SPSS Inc, Chicago, IL, USA). Data were expressed as mean ± standard deviation (SD). One-way ANOVA test with a post-hoc Bonferoni comparison was performed to compare the data among concentrations. Furthermore, a repeated ANOVA test for repeated cell growth was performed to compare the data among concentrations. Finally, a generalized linear model analysis was applied to show changes in data over time. All
Authors' contributions
HLZ participated in the design of the study and drafted the manuscript .QZ carried out atomic force microscopy and transmission electron microscopy, and drafted the manuscript .YLQ carried out the isolation and culture of hMSCs and their immunophenotype characterization. QQB carried out the analysis of hMSC differentiation. XAH carried out the MTT proliferation analysis. HTJ performed the statistical analysis. HWL participated in the design of the study and edited the manuscript. All authors read and approved the final manuscript.
This work was supported by grants from the the National Natural Science Foundation of China (Grant No. 30973127) and the Natural Science Foundation of Guangdong Province, China (Grant No.06300580).
Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues, Cloning in vitro and retransplantation in vivo
Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord
Term Amniotic membrane is a high throughput source for multipotent Mesenchymal Stem Cells with the ability to differentiate into endothelial cells in vitro
Human placenta-derived cells have mesenchymal stem/progenitor cell potential
Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood
Simultaneous activation of several second messengers in hypoxia-induced hyperpermeability of brain derived endothelial cells
Hypoxia induced proliferation of hematopoietic stem-cells in bone-marrow of mice
Hypoxia inducible factor-1 modulates gene expression in solid tumors andinfluences both angiogenesis and tumor growth
Hypoxia promotes murine bone-marrow-derived stromal cell migration and tube formation
Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs
Transcriptional profiling of human cord blood CD133(t) and cultured bone marrow mesenchymal stem cells in response to hypoxia
Proliferation and differentiation of bone marrow stromal cells under hypoxic conditions
Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan
Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells
Evidence for Transcriptional Regulation of the Glucose-6-Phosphate Transporter by HIF-1a: Targeting G6PT with Mumbaistatin Analogs in Hypoxic Mesenchymal Stromal Cells
Cobalt chloride and low oxygen tension trigger differentiation of acute myeloid leukemic cells: possible mediation of hypoxia-inducible factor-1alpha
Effect of desferrioxamine and metals on the hydroxylases in the oxygen sensing pathway
Involvement of mitochondrial- and Fas-mediated dual mechanism in CoCl2-induced apoptosis of rat PC12 cells
Stabilization of wild-type p53 by hypoxia-inducible factor 1 α
Effect of hypoxia on mesenchymal stem cells - review
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
Dynamic reorientation of cultured cells and stress fibers under mechanical stress from periodic stretching
A micro/nanoscale surface mechanical study on morpho-functional changes in multilineage-differentiated human mesenchymal stem cells
Promotion of osteogenesis through b-catenin signaling by desferrioxaminutes
Hypoxia increases Sca-1/CD44 co-expression in murine mesenchymal stem cells and enhances their adipogenic differentiation potential
Hypoxia and Stem Cell-Based Engineering of Mesenchymal Tissues
Mesenchymal stem cells in regenerative medicine: opportunities and challenges for articular cartilage and intervertebral disc tissue engineering
Connexin43 promotes survival of mesenchymal stem cells in ischemic heart
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs
Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells
Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells
Synergistic effects of CoCl(2) and ROCK inhibition on mesenchymal stem cell differentiation into neuron-like cells
Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation
Reorganization of nuclear factors during myeloid differentiation
Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells
Human adipose tissue is a source of multipotent stem cells
Biology of the stem cells in human umbilical cord stroma: In situ and in vitro surveys


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1994 Caperdonich 46% (LCBO# 262790)
— 1994 Caperdonich 46% (LCBO# 262790)
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Cavuto Guest Peterson Says Progressives' Citation Of MLK Legacy Is "Evil"
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- Posted In
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Titanic: The Artifact Exhibition [This exhibition has ended.]
Almost 100 years after it set sail in 1912, RMS Titanic the ship of dreams, has arrived on Singapore's sunny shores. This is the first time the travelling exhibition is making its appearance in Southeast Asia at the ArtScience Museum, Marina Bay Sands. Featuring over 275 authentic artifacts, 14 of which have never been seen before, visitors get a chance to step into extensive re-creations of different parts of the ship - the extravagant First Class cabins to the basic Third Class Cabins, the iconic Grand Staircase, Boiler Room and other locations on the ship. Learn about the life of Titanic at nine themed galleries, chronologically arranged to tell the story of its construction, life on board, its final moments during the ill-fated sinking, and the wreck's discovery. Visitors to the exhibit will also be thrilled to receive replicas of boarding passes containing names of actual passengers onboard the Titanic.
Titanic: The Artifact Exhibition runs from 29 October 2011 through to 29 April 2012.
Opening Hours: Daily 10am – 10pm (Last Admission at 9pm)
Address: ArtScience Museum, Marina Bay Sands, 10 Bayfront Avenue
Transformers: The Ride
The latest addition to Universal Studios Singapore's theme park family is the Transformers Ride, which debuted on 3 December 2011 after much anticipation. It is the world's first theme park attraction based on the film franchise of the same name, Hasbro's iconic Transformers brand. The motion thrill ride features 12 scenes, all of which showcase the most advanced evolution of immersive theme park entertainment with its hyper-realistic 3D high-definitive video and visual effects stimulators. In this latest ride, guests will be transported onboard advanced motion-based ride vehicles into the Transformers universe. They will also have a chance to be transported by an Autobot called EVAC (created exclusively for the ride) and combine forces with Optimus Prime and the Autobots in an ultimate 3D battle against the villainous Decepticons.
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Address: Located within the Sci-Fi City zone at Universal Studios Singapore, Resorts World Sentosa, 8 Sentosa Gateway
Maritime Experiential Museum
Learn about Southeast Asia's rich maritime history in the most innovative and interactive way at Singapore's first maritime museum, the Maritime Experiential Museum. With its distinctive maritime themed environment, the museum features more than 400 rare objects, bringing to life the Maritime Silk Route via life-sized historic ship replicas, shipwreck artifacts, interactive exhibits and educational programmes for all ages. It is also home to the world's first multi-sensory 360-degree Typhoon Theatre where visitors get to experience a simulation of a shipwreck right from their seats.
Opening Hours: Daily 10am – 9pm
Address: Resorts World Sentosa, 8 Sentosa Gateway
ION Sky Observation Deck
Spread across two levels, ION Sky offers a stunning 360 degrees panoramic view of the city from the highest point in the heart of Orchard Road, comprising both an observation deck and an event space. The wide open space is also aimed to host international runway shows, exclusive product launches, as well as high-end parties. It is also home to the urban-themed restaurant Salt Grill, owned by celebrity chef Luke Mangan. Boasting a height of 218 meters, the ION Sky is even higher than Marina Bay Sands SkyPark which is 200 meters high. Once at the top of the deck, visitors can get a closer look of the city surroundings in real-time through the observatory's high-tech Tellscope, promising an immersive viewing experience. Admission to the ION Sky observation deck is at $16 for adults and $8 for children.
Opening Hours: Daily 10am - 8pm with choice time slots
Address: Levels 55 and 56, ION Orchard and Residences, 2 Orchard Turn
Gardens by the Bay
An ongoing project by National Parks Board (NParks) to transform Singapore from a "Garden City" to a "City in a Garden", the Gardens by the Bay will comprise three distinctive waterfront gardens: Bay South, Bay East, and Bay Central, stretching across the mouth of the Singapore River. The largest of them all, Bay South, located right next to the Marina Bay Sands, will showcase the best of tropical horticulture and garden artistry, with a massive display of tropical flowers and coloured foliage. Visitors can expect a Conservatory Complex consisting of special themed domes (Flower Dome, "Cool Dry" and "Cool Moist" Domes), each displaying plants and flowers from different regions in the world. Another note-worthy feature is the Supertrees. Man-made trees with heights between 25 metres and 50 metres, they will be home to a wide variety of exotic ferns, vines and orchids, amongst other plants. The Supertrees are also embedded with environmentally sustainable energy and water technologies, providing shade and shelter in the day time, and lighting and ambience decoration at night.
Artist Impression of the Supertrees
The first phase of the Gardens Bay South is scheduled for completion by the end of 2011 and is targeted to open in June 2012.
After all the excitement and buzz, Swedish fashion retailer H&M have finally opened their first Southeast Asian flagship store in the heart of Singapore's shopping scene at Orchard Road. This means good news for those who regularly travel to other cities with H&M outlets for their H&M shopping fix. Located at Orchard Building and covering an area of 30,000 square feet spanning across three levels, the store is home to a wide variety of fashion and accessories for women, men, teenagers and kids. With its fashionable pieces and affordable pricing, it is set to be the next favourite shopping hotspot in town.
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Address: Orchard Building, 1 Grange Road
One of the newest kids on the block and named after London's exclusive fashionable address, Knightsbridge is home to upmarket retail outlets and flagship stores such as American youth apparel Abercrombie and Fitch, American classics Brooks Brothers, hip fashion brand Tommy Hilfiger and not forgetting British high street fashion retailer, Topshop. Like H&M, this stylish retail mall is located along Singapore's most prominent shopping district Orchard Road, and is housed within the luxurious Grand Park Orchard Hotel. Spanning across 8,000 square meters in retail size, it offers retailers unique concepts such as customizable retail spaces via double façade frontages, and direct street access – something that is entirely new in Singapore.
Opening Hours: Daily 10.30am – 10pm
Address: 270 Orchard Road
Louis Vuitton Island Maison
Housed in a glass and steel pavilion floating off Marina Bay Sands, the Island is Louis Vuitton's first Maison in Southeast Asia, and also Singapore's fifth store. The luxury landmark spans across four levels and incorporates both art and cultural elements in its retail space, housing an art gallery, bookstore, the exclusive Travel Room, private lounge, and an outdoor deck overlooking the Marina Bay cityscape for visitors to relax while shopping. There are three different ways to get to the Maison – from the outdoor bridge, arriving via a boat and disembarking at Island jetty, or through Marina Bay Sands Shoppes Basement 2, where a tunnel and ascending travelator will lead to the centre of the pavilion.
Opening Hours: Sun to Thurs: 10am – 11pm, Fri, Sat & eve of public holidays: 10am – 12am
Address: Crystal Pavilion North, #B1-38/39 & B2-36/37/37-A, Marina Bay Sands, 2 Bayfront Avenue
Avalon and Pangaea
Glittering additions to Singapore's nightlife scene are two of the hottest night clubs in the U.S and Europe, Avalon and Pangaea. Both opened after much anticipation in July 2011 at the Crystal Pavilions in Marina Bay Sands. Occupying the top two levels of the Pavilion and spanning over 17,000 square feet with impressive high fashion interiors, Avalon features some of the world's leading DJs and live concerts in a multi-sensory interactive environment. Pangaea on the other hand, with its red snake skin lounges and muted lighting, has a more intimate and sophisticated lounge club setting, akin to a European "bottle club".
Opening Hours: Avalon: Wed to Sun, 9pm till Sunrise, Pangea: Thurs to Sat, 8pm till Dawn
Address: Crystal Pavilion South, #B2-05 (Pangea) & Level 1 (Avalon), Marina Bay Sands, 2 Bayfront Avenue
KU DÉ TA Singapore
Designed and perched 200m above the city on an impressive 14,500-square-foot space on the roof of Marina Bay Sands, KU DÉ TA boasts a multi-tiered layout with several venues integrated into one – club lounge, outdoor bar with poolside terrace and a restaurant which offers both al fresco and indoor seating. Whether you're planning for a formal business lunch, a casual brunch, or a romantic dinner by candle light, guests will be pleased with the creative menus and freshest organic ingredients used at the restaurant helmed by award-winning chef Dan Segall. With Chef Dan's background in Japanese cuisine, expect to whet your appetites with his modern take on Asian dishes.
Over at the club lounge, club connoisseurs can look forward to an intimate clubbing experience on the indoor circular dance floor fit for 170 people, rocking to tunes rolled out by world-class DJs. If you'd much rather relax, try KU DÉ TA's signature cocktails while lounging about the spectacular infinity pool at the SkyDeck. Feeling adventurous? Head straight for the unique selection of drinks prepared by KU DÉ TA's very own resident mixologists and of course, enjoy the 360 degree panoramic view of the stunning Singapore city skyline from the SkyBar.
Opening Hours:
Restaurant & SkyBar: Lunch: Daily 12 – 3pm, Dinner: Daily 6pm till late, Weekend Brunch: 9am – 3pm
Club Lounge: Daily 6pm until late
SkyDeck: Daily 9am till late
Address: SkyPark at Marina Bay Sands North Tower, Level 57, 1 Bayfront Avenue
Joël Robuchon Restaurant and L'Atelier de Joël Robuchon
Much to the delight of food enthusiasts in Singapore, culinary sorcerer Joël Robuchon has opened his first outlets in Southeast Asia at the Resorts World Sentosa. Chef Joel, known for his passion for excellence, is the world's most decorated chef with 26 Michelin stars on his plate. Guests to both restaurants will be amazed by his repertoire of inventive French classics, fused with international influences from Asia and Spain. The 54-seat Joël Robuchon Restaurant is a fine dining restaurant serving traditional French cuisine, promising only the freshest dishes with produce sourced from around the world. L'Atelier de Joël Robuchon on the other hand is more experimental with its casual yet intimate setting, with the eating counter surrounding the entire kitchen where the guests' food is prepared, providing a full view of the chefs at work.
Opening Hours: Daily 5.30pm – 10.30pm
Address: Hotel Michael Level 1, Resorts World Sentosa, 8 Sentosa Gateway
International Cruise Terminal
Scheduled to be completed by the end of 2011, this new cruise terminal in Singapore's Marina South is set to make Singapore the one-stop cruise hub of Asia. Covering 28,000 square meters in size, which is one of the biggest in Asia, the new terminal will be ready to handle some of the world's mega cruise-liners by 2015, which previously docked at the older Pasir Panjang container port due to height and berth limitations at the current Singapore Cruise Centre.
Artist Impression of the International Cruise Terminal
The new location at Marina South has deep waters and a large turning basin with no height restrictions, enabling it to accommodate the next generation of cruise liners being built now. It will also be able to handle 6,800 passengers at a time and double Singapore's berth capacity.


text:
Stores »
I hope to win Because Mandy IS MY Sweet angel!!!! i love mandy and i love every song of Wild Hope
Lily Allen is amazing.
At first i didn't want to like it but you have to admit this is a really good cd.
This woman's voice makes the hair on the back of my neck stand up.
We are a community of shoppers sharing our best finds from across the web.
© 2017 ThisNext, Inc. ThisNext is a member of BUZZMEDIA Entertainment, a division of BUZZMEDIA.


text:
Share the Suspense ...
|Genre/Category: Crime, Thriller, Mystery
Usage: Standard Copyright
Ebook Format: PDF
Total Transfers: 8094
Register Here.
Sponsored links:
Even when life becomes somewhat complicated, murder is still murder. During the colsing days of the last millennium we anticipated something bad was going to happen, but what we did not expect was the change. Dead people stared to rise from their graves and folks stopped ageing altogether. It bagan tp rain and it has been raining ever since. Everyone believed it was the end of the world, things looked so bad. But a guy still has to make a living doesn't he?


text:
Riven by many deep and stupendous gorges, crowned by high and magnificent mountains neck-laced by a chain of lakes, water by majestic rivers, studded with burning deserts and great escarpments that plunge down from its highlands, Ethiopia remains a land of incomparable beauty.
But it is not simply the sheer scale of its physical beauty which characterises this land where the Blue Nile has carved one of the world's greatest and most awesome gorges.
Its ancient and medieval monuments, its proud and colourful cultures, and its varied and often unique wildlife set it apart from all else.
Ethiopia, dissected through its middle by the scar of the Great Rift Valley, is as old as time itself. It is a land claiming a history that goes back to the Old Testament.
It is the land of Sheba, and of Axum and Lalibela, of towering obelisks and the Ark of The Covenant - a land that was Christian before much of Europe and yet unknown to Europe for 1,000 years or more; a land that dwelt in limbo, forgotten by the world which it never forgot.
From the vast spread of Lake Tana, source of the Blue Nile River in the northern highlands, through the relics of Axum, seat of Sheba's legendary empire, down along the scorching inferno of the Danakil Depression, one of the world's lowest points, and high amid the wonders of the Simien and Bale Mountains to the untouched, game-filled wildernesses of the remote southern grasslands.
Click here for tours
Fact File
CapitalAddis Ababa
LanguageAmharic, Oromo, Tigrinya, Somali
ClimateThe predominant climate type is tropical monsoon. In Addis Ababa there are on average 7 hours of sunshine per day, meaning it is sunny for around 60% of the time. The dry season is the sunniest time of the year, though even at the height of the rainy season in July and August there are still usually several hours per day of bright sunshine. Most major cities and tourist sites in Ethiopia have a comparable climate.
VisaIssued on arrival at Bole International Airport
HealthAdvised immunisation against yellow fever, malaria, poliomyelitis, tetanus, typhoid, Meningococcal Meningitis and Hepatitis A
PassportsMust be valid for more than six months after proposed date of departure from Ethiopia
Time ZoneGMT +3


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Friday, October 30, 2015
Museum's Weekend Festival Focuses On NASA, Space Exploration
A three day festival, focused on NASA and space exploration, is running at the California Science Center through the weekend, according to the museum. According to the Center, which is centrally located in Los Angeles, its three day festival, Space Fest, runs from today through November 1st, and will feature a host of displays from NASA and demonstrations all focused on aeronautics and space exploration, talks from scientists and a NASA astronaut, and more. The California Science Center is home to the Space Shuttle Endeavor. The new exhibition also features a new film, Journey to Space 3D, narrated by Sir Patrick Stewart.


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Average Price: $36.13
Earth's Best diapers are the best diapers on the market, other than Honest Company but those are much pricier and not available at as many stores. Earth's Best diapers hold a lot of liquid, they are unscented, and are safe for your baby's sensitive skin.
I really really really wanted to like this brand. The idea of a diaper that was really eco friendly and great for sensitive skin. My problem was the fit and it leaked. My daughter doesn't have huge pee's and this one never held on. I found I had to change her more often than other brands because with a little bit of moisture, the diaper would almost fall off her bum.
These reviews are the subjective opinions of ChickAdvisor members and not of ChickAdvisor Inc.


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A few of my friends told me about this cave deep in the depths of the forest in our neighborhood. Apparently, the legend goes as this:
A cartographer was looking around the forest. He eventually found a cave. He went inside, wondering what it will lead into. Surprisingly, it was very large, except for the entrance. He noted that it was so huge, a waterfall could fit in here. But there was no waterfall. There wasn't even a source of light. It was very dark, and the air smelled rotten, hence the name Rotten Cave. He luckily had a lamp with him (This was in the 1800s, by the way), and was horrified to see dead rotting corpses in there. He rushed to the entrance to tell everybody of his discovery, but the entrance was blocked with stone. He tried furiously to get out, but it was no use. Then, he felt a cold breath down his neck. He was never heard from again.
I got to admit, it does seem like a pretty scary ghost story. But I didn't believe it. There must be a million dozen other ghost stories that were probably just like it. So when my friends asked me to go with them to the cave and check it out, I didn't hesitate.
Justin, David, Carter and I delved into the deep forest in the fall afternoon. All the trees had no leaves on them, and it was a pretty gloomy day. Good day for a ghost hunt, I thought. We were prepared for anything. First, we all had flashlights, with fresh batteries. We also had a horn so if one of us was separated, we would blare the horn so we would know where we were. We also had walky talkies, if there was a split entrance. We found the cave, and reluctantly, we went inside.
As the legend described, the air smelled foul. As if rotten eggs and sour milk was left here for years. We turned on our flashlights and off we went. We walked through the stoney entrance. There was a medium sized cracked that we could fit through. We squirmed into the tiny crack. Halfway through, we heard Carter scream. Turns out it was just a spider web. Carter was a scaredy cat. He didn't want to go in the first place, but instead of being at home, he decided to come with us.
We emerged through the crack to see the biggest space we have ever seen. But it wasn't beautiful. The walls were basically yellow and the air still smelled horrible. So we continued on. I started to feel a little frightened, mainly because our flashlights started to stop working. We went on to the rumbly rocks that we stepped over. That's when we noticed that Carter was gone.
We used our walkie talkies to communicate with him, but all we heard was static. We tried again and again, but nothing worked. The only words we could make out were "Stay....Get a...Hel-". We blared our horns, hoping that Carter would come. He didn't. We focused our attention on finding Carter. We walked all around the place like it was a maze. But we couldn't find him. He disappeared.
David proposed we get out of the cave, but Justin said that we should go forward. We still had more ground to cover, and we didn't even find any rotting bodies. I had to agree with him, because we might find Carter wandering in the depths of the cave. So we continued. The air started to smell even worse than it was before. How could that have happened? Soon, the smell got much more horrid. That probably means the bodies are close.
I started to shiver, as the air got colder. Then all of a sudden, everything became still. There was no smell, and it was very cold. We all felt it, and we all started to become frightened. David just kept on saying we should go. Justin said we had to find Carter. I told everybody to stop. I heard footsteps. We all thought it was Carter. Coming out of the darkness was a yellow rotting corpse slowly shifting towards us.
Naturally, we all screamed and ran away as fast as our young feet could go. David tripped on a rock as we continued to run. All we last heard from him was his screams echoing inside the cave. I kept on rushing towards the crack that we went through. I was completely horrified to see the crack was sealed off. I also realized that Justin wasn't with me. I was all alone, weeping and sobbing to myself.
I should have never have came here, I thought. I'm stuck here forever. I heard bones coming from all sides. I stood completely still, awaiting my doom. The last thing I felt was a cold breath going down my neck.


text: To get from Mesagne to Treviglio by train you can catch one of the 14 trains that everyday travel between these two cities. The first train to Treviglio leaves from Mesagne at 07:05 and the last train departs at 23:36. If you choose a local train the trip lasts on average 09:49 hours. For your trip from Mesagne to Treviglio by train you can choose to leave from one of Mesagne main stations: Mesagne On Wanderio you can compare all schedules, fares and railway companies to Treviglio and book the best itinerary starting from Mesagne.


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Discussion in 'Fan Zone' started by Coy, Dec 29, 2013.
As many as it takes to get you to leave.
I think he has the makings of a great coach. I also think that the game management thing is so grossly over the top that it is not funny. Every coach...and that includes your beloved Bellichick...has made mistakes that have hurt their team's chance of winning.
It started with the bogus ice the kicker and grew from there. Boniol was screaming for the TO because he saw something. Garrett trusted his kicking coach.
Now all you do is keep saying game management, no actual proof, just opinion. he runs the ball to eat clock and you ***** (NE), he throws it to get points before the half you *****(NO). He can't win. The other team makes a play, his team does not execute the play it does not matter...it is predetermined, it is Garrett's fault.
It is a witch hunt and I am sick of it.
Based on your standards Tom Landry would not have lasted past 1963 and if you say otherwise you are ******* liars.
You missed the point of the thread, this thread is not to praise Kelly as the nest coach ever and miracle worker, just to show what a good HC can do, unlike Garrett.
You still didn't answer the question and by the way this is 2014 not 1963 (51 years later), totaly different circumstances, totally different era and a totally diffenrent sport, very bad argument on your part, I mean common.
The problem with this franchise is the neutered head coaching position and the lack of accountability. It's impossible to hold the HC accountable because of the pervasive GM meddling and it's impossible to hold the GM accountable because he owns the team. Did Garrett pick the new defensive philosophy or put Callahan in charge of play calling? Did he choose the players on this roster or cause the decimation by injury? Some of his actions do look suspect, but clearly the larger problems that spill over into everything else are not his doing.
LOL funny how when it doesn't fit the agenda it is a bad argument.
Haha even funnier how you don't answer a question because you are inclined to take Garrett over Belichick or maybe even over every other HC in the NFL and that buddy is just flat out ridiculous, not even worth discussing, I really hope Garrett pans out, I really do, I'll be pulling for him because I love my Cowboys, I just think it's gonna be another wasted year, anyway, Happy new year.
Why answer the question you guys never do all you do is spin and repeat the same crap over and over.
Don't bother, I alredy know your answer, that's why I said, "not worth discussinhg"
No you think you know...just like the rest of your football opinions.
Lol, your funny man. All hail to Garrett.
Ooh, burn!
BZ, just out of curiosity, how long would you give Garrett? What would it take for you to pull the plug?
The same as any coach, taking a step back under optimum conditions. And no playing 26 different players in your front 7 is not optimum conditions. Neither was playing your #4 center with both guards hurt and losing your NT, both inside LBs and having Ware play with one arm the second half of the season.
If people would honestly look at the circumstances instead of blind hatred they would realize that being 3 points from the playoffs this season was actually damn good coaching.
While I disagree about Garrett, I respect your opinion and hope he becomes a successful coach next year. Thanks.
There is never going to be a season where injuries don't play a major factor in a team's performance. Green Bay lost their quarterback and best WR for a good chunk of the season and still made the playoffs. When will the excuses stop with you and your boy Garrett?
Again a terrible comparison. GB won the division because Trestman gave games away by playing Cutler and Detroit just fell apart going 1-6 down the stretch.
Green Bay's making the playoffs wasn't because they were coached well enough to overcome key injuries (they only won 2 of the 8 games Rodgers missed, and each of those was only by 1 point). It was really because Rodgers's injury kept him out of 8 games instead of 9.
Bookmarked for when they finish 3rd or 4th in the division next year.


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Discussion in 'XenForo Questions and Support' started by flowerpot132, Mar 8, 2016.
How long does it take for the new posts count to reset?
A month?
What do you mean by reset?
Unread posts newer than the value set for 'Read Marking Data Lifetime (Days)' will show as new posts.
I mean if a user doesn't login for ages, then the new posts count will show as 100s. Can it not do that and just show the new posts from say the last month?
Change the value for the option I mentioned to 30.
Ok it was already set to 30 days. So if I login as a user and see 300 posts unread. If I wait 30 days then log back in, that will be no unread, presuming there have no new posts in that 20 day period.
Thanks. One last thing... can that user log in inbetween the 30 days and that 30 days still count, or does it reset everytime they log in?
It is based on the post date relevant to the actual date.
Ok I think there might be a problem with my forum then. It is not displaying that behaviour?
If I go to new posts, it is showing posts (as unread) that are older than 30 days.
I doubt there's a problem. Can you screenshot what you're seeing and highlight what the problem is? Note that this is all based on last post date.
Separate names with a comma.


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You purchased your M35h because its a reputation for safety and Japanese engineering appealed to your senses; so why would you be okay with second best when it comes to great quality auto parts? When your Infiniti M35h was born, it was destined for a person with discerning taste who wants unique styling in their automobile. Auto enthusiasts appreciate the a reliable name built into each Infiniti, and understand that only the highest quality repair M35h parts will suffice when repairs are needed. While it's not hard to buy replacement parts for Infiniti vehicles, these days the necessity for nothing but the highest quality are required to fix your car or truck.
The fuel in our engine would not be combustible without mixing with air first. Your Infiniti M35h has an internal combustion engine that mixes fuel with air before it detonates, and an air filter needs to remove dust and debris before it is ignited. Without an air filter or air cleaner in your engine, your fuel would be filled with particulates from outside. This will clog fuel injectors and lower the octane in your fuel; making it difficult for your engine to generate power. And unless your Infiniti M35h air filter is replaced regularly your engine will be robbed of the necessary air flow needed to maintain optimum fuel efficiency. Clearly, replacement air filters are a necessity for everyone.
At Car Parts Discount, we have real customer support agents on the phone with enough expertise to help you buy the appropriate Infiniti M35h part for your needs. Buying new Infiniti parts from www.carpartsdiscount.com means you are taking advantage of the highest quality original and aftermarket replacement M35h parts on the internet. Given all the hassles associated with fixing broken parts on your car or truck, let www.carpartsdiscount.com be the most authoritative website you go to make purchasing it easy. All the Infiniti M35h Air Filter & Cleaner parts on this page will fit vehicles made in 2013, 2012. Choose your year below to refine your search.


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Go Red for Women
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| Clip Of Mitt Romney Town Hall Meeting This clip, title, and description were not created by C-SPAN.
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July 31, 2012
July 31, 2012
Mitt Romney on a national ID card2011-10-20T21:02:11-04:00https://images.c-span.org/Files/22d/20111020210324001_hd.jpgMitt Romney talks in 2011 aout the need for a national ID card.
Mitt Romney talks in 2011 aout the need for a national ID card.
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Mitt Romney was interviewed by New Hampshire Union Leader editors. Questions focused on his economic plans and his role in…


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Aww! Thank you, Linda! You are such a sweetheart!!
You look awesome!!
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Wow ladies! You sure know how to make me blush...thank you!! I look forward to learning more from you mom's! :O)!!
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I am an expectant mother, perhaps just like you, who was looking for an opportunity to work from home for a legit company. And I've found it! Not only are the rewards financial but more so, health wise. It is doable with hard work and determination!


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|Doji Koin
|Doji Soh
Doji Koin was a Crane magistrate and ambassador who was sent to the Badger Clan.
Family Edit
Koin was Doji Soh's younger brother. [1]
Discovery of Ryoshun's Grave Edit
In 1166 the Badger Clan had bareky been able to pay their taxes, so the imperial families sent a functionary, Miya Tsurugi, to decide whether the Badger's minor clan status should be revoked. Koin came there to claim the right of the Crane to defend the Ichiro lands if the Badger lose their Minor Clan status. To be sent so far from home he must had offended someone important or believed he could prove himself there. Koin's shugenja advisor at that time was Asahina Hira, who was considered by Koin as a helpless old blind man. Upon Hira's discovery that the Badger guarded the Ryoshun's Grave, the tomb of the Tenth Kami, Ryoshun, Tsurugi informed the Badger had been fulfilling Hantei's charge, and Koin pledged the Crane Clan to pay the small clan taxes the next year. Hira proved he was enlightened and became the Keeper of Void. [2]
The Badgers Live Edit
In 1170 when the Army of Fire threatened to overwhelm the Badger forces, Koin was the last remaining guest. Badger Champion Ichiro Kihongo would not have the Badger abandon their ancestral duty, even if it meant facing his clan's extinction, and asked Koin to leave. To save both the Badger's honor and his future, the Crane asked Kihongo for an honor escort - which would include all Badger children. By the time Kihongo agreed, the yobanjin scouts had come to close to be circumvented. Koin, with the help of his yojimbo Daidoji Sugoru, managed to find a Badger sentry whose father had dug a mine shaft straight into the foundations of Shiro Ichiro. With this escape route, the Badger's future was saved. [3]
External Links Edit
- Doji Koin (Code of Bushido)
- ↑ Doji Koin (Code of Bushido flavor) and Doji Soh (Code of Bushido flavor)
- ↑ The Touch of the Void by Shawn Carman
- ↑ The War of Dark Fire, Part 3 by Shawn Carman
|This Crane Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
2704 Erie Ave. Send to Phone
513-321-6221 | Make Restaurant Reservations | Menu
OpenOpen daily
- Parking available
- Dress code: Casual
- Kid-friendly
* Click here for rating key
Graeter's Restaurant Review: Graeter's is available in plenty of Cincinnati's neighborhoods, and you can even get it from the frozen-food aisle at the grocery. But there's something about Hyde Park Square and its promenade that makes the ice cream here taste even better. Enjoy your sweet treat outside on a bench by the fountain and take in the sights and sounds of summer. Graeter's uses a French pot process to make its creamy concoctions. Black raspberry chip is a favorite, with big chunks of chocolate throughout. The blueberry pie flavor is irresistible, and the waffle cones are a must-try. You can even take some home in a dry-ice travel pack.
Find out which restaurants and chefs top the list of GAYOT's 2016 Annual Restaurant Awards.


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Sport Hg Compressive Large Microperforated
Product description
Compressive large microperforated pants unisex. With Climatherm thermoregulation technology and manufactured without seams for maximum comfort. The cloth is hypoallergenic, absorbs sweat and evaporates it quickly. It eliminates all kind of odor. The athlete is always kept dry, clean and at the best temperature. With differencial compressive system in order to improve blood flow. With carbon fiber: it eliminates static electricity and reduces muscle charges. That is helpful to reduce lactic acid and avoids risk injuries and strains. Recovery times are reduced too.
- Compressive large microperforated pants unisex belonging to our Active Plus Carbon line, with differencial compressive system. It offers a high pression on strategical zones,
- Great security sensation. Injury risk reducted.
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- Composition: 8% Elastane, 7% Microfiber PA, 5% Resistex Carbon, 80% Climatherm
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Data Rescue 3 has and can be used to recover data from a floppy disk or CD/DVD media in certain cases. We do not officially support this, however if your able to see your files by using the demo and our steps below to preview or recover a sample file give it a shot.
A floppy disc can not be directly scanned by Data Rescue 3. However if your able to clone the contents of a flash or SD drive to another device or make a .dmg file , it can be scanned afterwards. You can do this with a external floppy drive and disk utility. Apple's Disk Utility can make a .dmg file out of the floppy disk. For more info on creating a disk image from a drive, visit Apple Support document
Afterwards scan the .dmg file with Data Rescue and 3 follow the steps below,
1) Go to the "Data Rescue 3" menu at the top of the screen and select "Preferences". 2) Click on the check box for "Enable Expert Features", then close the Preferences window. 3) Ensure you are within the "Detail View" for Step 1 to see the list of devices. If you are in Arena View (with the animated interface) click on the "Detail View" on the bottom left corner to change views. 4) Go to the Expert Menu at the top of the screen and select "Add file to scan…". This will display an "Open" prompt for you to select a file. 5) Select the file in the prompt and click "Open". 6) You will notice the file will appear in the "Select a Drive/Volume" list. You may proceed with your scan of the file from there.
Data Rescue is capable of scanning CD/DVD media based on file patterns to rebuild any common file types it detects, such as JPEG, MP3 Audio, and Word Doc. However, Data Rescue 3 does not display the CD/DVD media as a device to scan by default. You may enable the selection of CD/DVD's by enabling these special features within the Demo of Data Rescue 3. Steps on enabling all devices: 1) Go to the "Data Rescue 3" menu at the top of the screen and select "Preferences". 2) In the "General" tab, click on the click on the "Enable Expert Features" check box to turn on this feature. 3) Select the "Scan Engine" tab. Hold the "Control" key and click on the category titled "Scanning". 4) On the right side of the list, enable the check box titled "Show all devs", then close the Preferences. (Please note if the right side of the list does not contain "Show all devs", highlight a different category temporarily, then hold the "Control" control key and click on the "Scanning" Category once again). 5) Close the "Preferences" window. 6) Refresh the hard drive list by going to the "Expert" menu at the top of the screen and select "Refresh Volume List".
Once the list is refreshed, the CD/DVD media will be available to scan. From here, you may try the demo to scan the media with a Deep Scan, and any files found will be listed within the "/Reconstructed Files/" folder to review. Simply highlight any files of interest and click on the "Preview" button to see if the files display properly. If it provides you with the results you are looking for, you may purchase the full version of Data Rescue 3 here to continue with your recovery.


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When developing another patch series I had a temporary state in which git-clone would segfault, when the call was prepared in prepare_to_clone_next_submodule. This lead to the call failing, i.e. in `update_clone_task_finished` the task was scheduled to be tried again. The second call to prepare_to_clone_next_submodule would return 0, as the segfaulted clone did create the .git file already, such that was not considered to need to be cloned again. I was seeing the "BUG: ce was a submodule before?\n" message, which was the correct behavior at the time as my local code was buggy. When trying to debug this failure, I tried to use printing messages into the strbuf that is passed around, but these messages were never printed as the die(..) doesn't flush the `err` strbuf.
When implementing the die() in 665b35ecc (2016-06-09, "submodule--helper: initial clone learns retry logic"), I considered this condition to be a severe condition, which should lead to an immediate abort as we do not trust ourselves any more. However the queued messages in `err` are valuable so let's not toss them out by immediately dying, but a graceful return. Another thing to note: The error message itself was misleading. A return value of 0 doesn't indicate the passed in `ce` is not a submodule any more, but just that we do not consider cloning it any more. Signed-off-by: Stefan Beller <sbel...@google.com> --- Here is the last patch of the series as an independant bug fix applicable on master. Thanks, Stefan builtin/submodule--helper.c | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/builtin/submodule--helper.c b/builtin/submodule--helper.c index 6f6d67a..bd7cce6 100644 --- a/builtin/submodule--helper.c +++ b/builtin/submodule--helper.c @@ -748,8 +748,12 @@ static int update_clone_get_next_task(struct child_process *child, if (index < suc->failed_clones_nr) { int *p; ce = suc->failed_clones[index]; - if (!prepare_to_clone_next_submodule(ce, child, suc, err)) - die("BUG: ce was a submodule before?"); + if (!prepare_to_clone_next_submodule(ce, child, suc, err)) { + suc->current ++; + strbuf_addf(err, "BUG: submodule considered for cloning," + "doesn't need cloning any more?\n"); + return 0; + } p = xmalloc(sizeof(*p)); *p = suc->current; *idx_task_cb = p; -- 18.104.22.1683.gd6329be.dirty -- To unsubscribe from this list: send the line "unsubscribe git" in the body of a message to majord...@vger.kernel.org More majordomo info at http://vger.kernel.org/majordomo-info.html


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Six-Month Practical Filmmaking - Berlin
Six-Month Practical Filmmaking is designed for those wanting a fast-track into a career as a screen creative. It offers an intensive and highly practical immersion in the skills required to work in the moving image industry.
Taught by industry professionals, Six-Month Practical Filmmaking provides students with the confidence to enter employment in the creative industries, instilling a foundation in the core elements of filmmaking. They will learn how to write a screenplay, direct scenes and how to produce their own filmed content. On completion of the course, students will graduate with a showreel of their work.
The six-month programme consists of three terms. During Terms 1 and 2, students will be trained in all key aspects of filmmaking through a combination of intensive practical workshops and a series of creative exercises, escalating in scale as they go through the programme. Following the learning from the foundation programme in Term 1, students learn the advanced skills in screenwriting, cinematography and sound, fiction directing and producing, documentary production, editing and postproduction across Term 2. In Term 3, students will make their own film and perform key roles on set on other students' films.
- Learn how to interpret a script and create a storyboard
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- Be able to record sound and use it creatively to tell a story
- Be able to cast a film and direct actors
- Manage a small crew and direct using the correct on-set procedures
- Be able to use professional editing software and understand the key postprodution elements of film finishing
Minimum Age: 18 at start of course
Entry Requirements: Our Practical Filmmaking course is open enrolment and there are no entry requirements. However in order to get the most out of the course, you should be able to both speak and write in English to a reasonable standard.
This school offers programs in:
- English


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Dialogue Mette Laier
About the Book
Dialogue, a jewelry book by Mette Laier.
Jewelry inspired by a trip to the U.S. Virgin Islands, made in fair-trade gold, silver and corals.
Features & Details
- Category Crafts & Hobbies
Small Square, 7×7 in, 18×18 cm
24 Pages
- Publish Date Feb 11, 2014
- Language English
- Tags fair-trade gold, necklaces rings, Jewelry, coral, silver
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Information not available
Judgment of the Court (Grand Chamber) of 21 December 2016
European Commission v Hansestadt Lübeck
Appeal — State aid — Airport charges — Article 108(2) TFEU — Fourth paragraph of Article 263 TFEU — Decision to initiate the formal investigation procedure — Admissibility of an action for annulment — Person individually concerned — Legal interest in bringing proceedings — Article 107(1) TFEU — Condition relating to selectivity
Case C-524/14 P
published in the electronic Reports of Cases (Court Reports - general)
Judgment of the Court (Second Chamber), 3 July 2014
Eycke Braun v Land Baden-Württemberg
Request for a preliminary ruling from the Amtsgericht Karlsruhe
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Case C‑524/13
Order of the Court (Sixth Chamber) of 14 November 2013
TeamBank AG Nürnberg v Office for Harmonisation in the Internal Market (Trade Marks and Designs)
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Case C‑524/12 P
published in the electronic Reports of Cases (Court Reports - general - 'Information on unpublished decisions' section)
Judgment of the Court (Sixth Chamber) of 6 September 2012.
Lowlands Design Holding BV v Minister van Financiën.
Reference for a preliminary ruling: Hoge Raad der Nederlanden - Netherlands.
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Case C-524/11.
Judgment of the Court (Fourth Chamber), 8 March 2012
European Commission v Portuguese Republic
Failure of a Member State to fulfil obligations — Common system of value added tax — Directive 2006/112/EC — Articles 296 to 298 — Common flat-rate scheme for farmers — Flat-rate compensation percentage set at nil rate
Case C-524/10
Judgment of the Court (Fourth Chamber) of 22 December 2010.
Ville de Lyon v Caisse des dépôts et consignations.
Reference for a preliminary ruling: Tribunal administratif de Paris - France.
Preliminary rulings - Aarhus Convention - Directive 2003/4/EC - Public access to information in environmental matters - Directive 2003/87/EC - Scheme for greenhouse gas emission allowance trading -Regulation (EC) No 2216/2004 - Standardised, secured system of registries - Access to data on greenhouse gas emission allowance trading - Refusal to report - Central administrator - Administrators of national registries - Confidential nature of the data held in the registries - Exceptions.
Case C-524/09.
Judgment of the Court (Third Chamber) of 11 December 2008.
Commission of the European Communities v Republic of Austria.
Failure of a Member State to fulfil obligations - Articles 28 EC and 30 EC - Registration of old used vehicles previously registered in other Member States - Technical requirements with regard to exhaust emissions and noise pollution - Public health - Protection of the environment.
Case C-524/07.
Summary publication


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A/N: Hi again everyone. Good to be back in the land of the submitting. Anyway, this little story is about Lycan - which is one big leap outside my Naruto comfort zone. In case you're still a little confused, this is NOT part of the "Friends" series. Anyway, some background.
I wrote this story over a year ago after seeing Underworld. This story is NOT about that. You will see no familiar characters, and (hopefully) I didn't use any character names from that movie. If I did, it was completely coincidental, because I don't know enough about it to write a fic about it. I just used their "world" if you will, to create a story about the Lycan race and what happens when a future leader is duty bound to choose a mate. This story is told from the point of view of a prospective "mate." Must warn you, this is a dark, sexy, intense, and sometimes violent story. You have been warned. All characters here were made up by me, and therefore belong to me. The "world" I guess would belong to Underworld. So with that, I hope you like it. Enjoy!
My name is Rhiannon and I am among the chosen. When I was born, my mother chose my name carefully. It means "Great Queen" in Welsh, or so I'm told. I was born to be a great queen some day, but first, I had to be chosen for the position by a man who would be the King, or Chief of our clan. I was amongst the chosen, and it was my turn to meet with the man who would be Chief.
My mother had been waiting for and fearing this day her whole life. I just wanted to get it over with. As she poked decorative combs and pins into my hair I asked her, "Mother, am I ready yet? I'm really tired of all this primping."
"Just a few more pins. There, you look ravishing. I hope Kylon thinks so."
Kylon. The murderer. He was next in line to lead our Lycan clan and be Chief when his time came. Presently, he was of age to begin making decisions alongside his father, so it was deemed that it was time for him to pick his mate. There were requirements. Blood line qualities, feminine qualities, strength, and more. But he was still a murderer.
I hadn't seen him since we were children. He'd been sheltered by the whole clan, and had become an important member, strong and vicious as any Lycan should be. But he mercilessly killed three women who were offered to him as potential mates. Apparently, they were bred specifically for him to choose from, and he chose to kill them all. I was also conceived for this purpose. I was born two years after he was to the whole clan's joy. My blood line is superior to the other girls, yet they were offered to him first. I didn't care – he killed them when our clan was at its lowest population ever. They could have been valuable members, but instead, they're dead.
Apparently, each ritualistic meeting went the same way. The women were invited to meet him over dinner, where they'd talk for a while, they'd get to know each other, and then the animal magnetism took over. Each had given themselves to him, each of whom he took greedily, and when he finished with them, he'd marked each with a bite on the shoulder to mark them as "his." Then he rolled them over and tore out their throats. He said each one was bred inferiorly for some reason or other. It is said he'd know when he found the right mate. There were a few more in line waiting for their turn with him. Who wouldn't want to be his mate? All the power, protection and prestige that goes along with entering the first family. I don't want it. I never did. And I certainly didn't want him.
I heard my mother say, "Rhiannon, listen to me. It's almost time for you to go for your meeting. Please, listen to him. Do not speak until you are asked to. Please him carefully and he may choose you."
"And if I don't or he doesn't?"
"I fear this may be the last time I see you my child." I looked her in the eye. I wasn't afraid to die. I was a well bred strong Lycan. If it was my fate for him to rip out my throat after having his way with me, I could not change that. Time would tell.
I stood at the knock at the door. They had come for me. "Rhiannon, may the gods bless you. Be your best. May all luck be upon you."
I hugged her, "Thank you Mother. I'll do what I must."
I opened the door and found two men waiting there for me. One said, "Kylon will see you now Rhiannon."
"Thank you." They led me to Kylon's dining chamber where he "interviewed" his prospective mates. They opened the door and led me in where I was seated at the end of a long table.
"Wait here. He won't be long. Make yourself comfortable, but don't leave."
I nodded and sat at the end of the table. It seemed like an eternity. I sat looking around at the old dusty pictures on the walls of the ancient Lycan who had passed on before us. Kylon's grandfather was among them. His father was depicted as well, but he was still very much alive and currently governing our clan. I couldn't tell if there was a picture of Kylon himself, because I didn't know what he looked like now. I hadn't seen him since we were children. I was curious to see him again. Lycan are very attractive people. It's bred into us. I was sure he would be handsome. I waited and looked around some more. I had to remember my manners when he did arrive, or I might not be breathing long.
The door at the far end of the room opened and in he breezed. I told myself to keep my heart rate steady, as we Lycan can sense fear, apprehension, stress, and excitement. I held myself steady as I looked at him. He was impossibly handsome. He still had some features that I remembered from so long ago, but he had grown into his manhood spectacularly. Inside I was breathing heavily, wanting him to choose me. I held my heart rate even. He spoke as he walked down the table to greet me. I stood. He extended his hand, "Rhiannon right? I remember you from when we were kids. You have turned into an absolutely striking woman." I bowed slightly, not looking at his eyes – they were a white blue – and said, "Thank you Kylon. You haven't changed much since we were kids."
"Oh, I believe I have. Come sit. Talk with me, while we eat." Platters of meat were bought out to us and dark red wine. We ate silently; I spoke not a word to start a conversation, as I heard one girl was killed because she talked too much. I was to speak only when spoken to. I decided to let him lead the conversation. Finally he said, "Rhiannon. You and I both know why you're here. I want to go over some things with you before our evening proceeds."
"Certainly Kylon."
"First of all, I wanted you to know that you were actually my first choice. You were my family's first choice for me. But there was some rumor of you being stubborn, willful and possibly difficult." I held my heart beat steady. I gazed at him. "Now that I've been introduced to the others in line after you, I demanded of my father that we go back and have a look at you. I'm glad I did. You are very pleasing to the eye."
So I was the first choice? Interesting. I growled slightly, "Thank you Kylon."
"What is it? Something the matter?"
"May I speak freely?"
"I hate what you did to them."
"Who? The others."
"Why? They were essentially bred for me, but they weren't right for me. What else could they have done?"
"Our population is as low as it's ever been. They could have been trained, given jobs to do, taken care of this mansion, anything. But now they're dead and we're down three more in the clan. I see their killings as senseless."
"Are you calling ME senseless?"
"I call your killing them senseless."
"I see." I stared at him unwaveringly. "You are willful, I will give you that. You're brave to even speak to me that way. I could eliminate you right now and move on to potential mate number five."
"If you do, you won't really get to know me Kylon. You won't know the rest of me, which I'm sure you want to experience if the scent of your testosterone is any indicator."
"You can smell that? Very good. It's true. You fascinate me, and your attitude is entertaining. But are you willing to serve me? Be my mate? What if I chose you, would you accept me?" He waited. "Well? I'm waiting?"
"I'd need time to think about it."
"What's there to think about? If I chose you, it'd be an honor! You're life would be set!"
I was pushing some very sensitive buttons but I had to know.
"What did the other women answer to your question?"
"They all said they would accept me."
"And yet you killed them all." He was silent. He thought to himsef for a moment, then he scowled a bit showing his teeth. His white/blue eyes grew angry.
"I am saddled with the responsibility of doing what is best for me."
"Not what's best for the entire clan?" I wished I could shut up.
"Willful is not the word for you. Leave. Be gone. I want you gone." He raced up to me and got right in my face, an inch or two from my nose. I smelled his testosterone and instantly reacted. "You will return to me tomorrow night and we will finish this. One way or another, we will finish this." He growled and turned, flying to the door. He turned when he reached it, "You reacted to me when I was close to you. I felt your wantonness. Be here tomorrow." He left. As soon as he was gone, I lost control of my heart, and it pounded in my chest.
I went back to my mother. She was thrilled, "He chose you! Rhiannon! That's wonderful!"
"He didn't choose me."
"Then how are you alive?"
"I think I infuriated him. He wants me back there tomorrow to 'finish things,' as he put it."
"Oh, Rhiannon. We should leave. We can run, I can hide you for a while until he finds you and,"
"Mother, if this is going to be my last night on this earth, I'd like to go clear my head. It's my own fault if I've upset him. He is quite cocky though. I'm going for a walk. I'll be back in a while."
"Be careful child. He may send people after you if he's really angry."
"Somehow, I don't care if he does. I spoke my peace and my fate rests in his hands. Don't worry. I feel I'll be back soon." I left and walked alone in the night air. The night was cool and I was able to collect my thoughts and calm my nervous heart. I re-thought what I'd said. I must have been an idiot to argue with such a powerful person as Kylon. He was so beautiful, manly. I loved looking at him. But I wouldn't bow to him. He just may kill me tomorrow, but tonight, I still belonged to me.
On my way back to the mansion, I felt someone watching me. I smelled the testosterone. Kylon was watching me. I ignored him and kept moving. Was he going to kill me now? Let him try. I'd fight him tonight if I had to. I turned and looked into a shadow, looked right at him menacingly and walked right by him. He didn't move. I entered the mansion and soon went to bed.
A/N: Ok so far? This is a big change for me, so I'd be interested in what you think.
Next up: The second meeting. See you soon!


text:
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