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Artist:Jo-El Sonnier
Title:Have A Little Faith
Year Of Release:1989
Genre:Blues, Cajun & Zydeco, Country
Quality:Flac (tracks, log, .cue)
Total Time:36:52
Total Size:224 Mb (covers)
WebSite:Album Preview 16:59
01. The Scene Of The Crime
02. If Your Heart Should Ever Roll This Way Again
03. Ooh, Ooh, Ooh
04. Have A Little Faith In Me
05. I'll Never Get Over You
06. The Hurt In My Heart
07. Solid Gold-Plated Fool
08. Blue, Blue, Blue (Blue, Blue)
09. Walls
10. Evangeline Special
The emphasis here lies more heavily on ballads, as Sonnier discovers John Hiatt and delivers penetrating versions of his "Have a Little Faith" and "I'll Never Get over You." The album also includes a remake of Iry LeJeune's 1945 "Evangeline Special" and a straight-country single in "If Your Heart Should Ever Roll This Way Again."
Premium Jo-El Sonnier - Have A Little Faith (1989) Fast & Anonymous
(Buy premium account for maximum speed and resuming ability)
(Buy premium account for maximum speed and resuming ability)


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Many Fear UK Housing Plan Will Create Sprawl
"Government plans to build three million new homes by 2020 will create an "unsustainable urban sprawl", the Tories have warned."
"'His own government officials are planning to let rip with the concrete mixer and add to unsustainable urban sprawl,' Shadow planning minister Jacqui Lait said of new Prime Minister Gordon Brown."
"She added: 'Local residents will be powerless to stop the unelected bureaucrats building the sink estates of the 21st century.'"
"The Financial Times newspaper reported that the Government Office for the South East had set a target of building up to 38,000 new houses annually, in line with last month's Housing Green Paper."

subset: commoncrawl


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Wilson Engineering employs the most qualified and experienced staff of engineers and surveyors. Every engineer in our staff is a licensed Professional Engineer (PE) or Engineer-In-Training (EIT) in the state of Washington, Alaska, Oregon, California, or Hawaii. Our surveying staff consists of Professional Land Surveyors (PLS) and Land Surveyors In Training (LSIT) licensed in Washington. If you are interested in starting a career with Wilson Engineering, please send us your qualifications for future available positions.
Current Available Positions:
Civil Engineer (Wastewater Emphasis)
We have two job openings for civil engineers with education and experience in designing wastewater treatment and conveyance systems. Skills we are looking for include working well with coworkers and clients, problem solving, knowledge, analytical, verbal, writing, and CAD. We have a superb team of positive people, a great working and learning environment, and competitive salaries/benefits. Bellingham is a great place to work, live, play, and raise a family.
- Engineering design and/or project management for a wide variety of municipal wastewater treatment and conveyance systems involving treatment, water reclamation, pump stations, and sewer rehabilitation.
- Planning, evaluation and selection of preferred wastewater conveyance and treatment alternatives.
- Conceptual and detailed design calculations and analyses.
- Detailed engineering reports and supporting documents.
- Construction drawings, specifications and cost estimates.
- General civil engineering design
Experience : Position No. 1 - minimum 8 years; Position No. 2 -
minimum 4 years
Education : BS or MS in Civil or Environmental Engineering
Compensation: Salary depends on experience
Benefits: Health Insurance, Generous Vacation, SIMPLE IRA Match
Send Qualifications to:


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B00RKQFU0M Used good or better, we ship best copy available! Book Only. Expedited shipping is 2-6 business days after shipment, standard is 4-14 business days after shipment. Used items do not include access codes, cd's or other accessories, regardless of what is stated in item title. If you need to guarantee that these items are included, please purchase a brand new copy. Bookseller Inventory #
Title: Photograph 51 by Ziegler, Anna (2011) ...
Publisher: Dramatist's Play Service
Book Condition: Good
Book Description Book Condition: Good. Book Condition: Good. Bookseller Inventory # 97808222250894.0
More Information About This Seller | Ask Bookseller a Question


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Taye Biddle has barely cracked Jon Gruden's complicated playbook. But there are already questions about whether Biddle may be able to help the Tampa Bay's complicated kick and punt return situation.
The Buccaneers signed Biddle on Sunday, one day after the Carolina Panthers cut the second-year pro.
Biddle found out about 2 p.m. Sunday that he would be coming to Tampa. After a quick trip through the locker room to meet his new teammates Monday morning, he was on his way to pick up his playbook.
"It was weird at first. But after getting in here and meeting everybody, it feels pretty good," Biddle said. "I'm already used to it. I like what I see."
The Buccaneers must have liked what they saw out of Biddle this preseason. He made a splash in the Panthers' preseason contest against the New York Giants on Aug. 10, catching two passes for 108 yards and two touchdowns in the game. But it wasn't enough to earn Biddle a spot on a roster jammed with receivers.
Gruden said the addition of Biddle gives the Bucs three speedy deep threats, along with Joey Galloway and David Boston.
"The guy can run, really run," Gruden said. "He has some return ability and a guy that has rare speed. We're excited to work with him. You can never have enough of that."
Biddle played in just two games for Carolina last season, registering three catches for 37 yards. He does not have a regular-season kickoff or punt return to his credit, and according to stats compiled by NFL.com, he did not have a kickoff or punt return in the preseason.
But his speed and receiving ability automatically makes him a candidate to help in both areas. After the release of Mark Jones on Saturday, Gruden told the media that Ike Hilliard, Joey Galloway and Phillip Buchanon would form a "return" committee.
Biddle admitted that he's more comfortable with punts at this point.
"Punts are more my specialty, but I did both," Biddle said.
Of as much interest to Biddle is the opportunity to work with Galloway, who at age 35 is still one of the league's fastest players. Biddle is eager to compare Galloway to Steve Smith, the Panthers' deep threat and a mentor to Biddle since the Mississippi product arrived in Carolina.
"I learned a lot of the fine, little techniques that he (Smith) does, the stuff that he sees through his eyes," Biddle said. "He would sit down with me and break things down during meetings. He taught me how to watch film. There are little things that he does to actually master (the deep route). I'm sure there are some techniques that Joey does, too, so I'm going to try and pick that up and make my game better."
Matthew Postins covers the Buccaneers for BucsBlitz.com and the Charlotte Sun-Herald in Port Charlotte, Fla. He is a member of the Pro Football Writers Association and has won national awards for his Buccaneers coverage from the PFWA, the National Newspaper Association and the Associated Press Sports Editors. He is also a contributor to the Scot Brantley Show from 4-7 p.m. weekdays on WHBO 1490-AM in Tampa-Clearwater.


text: File:Stacy and Ginger.jpg Stacy and Ginger looks very similar, just look at those pictures above... Could they be sisters, or at least family in some way? The only difference (not counting age, lenght and clothes) with them is... That Ginger's ears can be seen. That Stacy got wider eyebrows. Retrieved from "http://phineasandferb.wikia.com/wiki/User_blog:Amindis/Stacy_and_Ginger_-_sisters%3F?oldid=402493" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Pages with broken file linksInactive blogs


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Christie Stevens – Neighbor Affair
Released: August 20, 2013
Christie Stevens is chillaxin' by the pool with some fresh-made sangria she concocted for her and her boyfriend, but when she calls him and tells him she's awaiting his arrival, the bastard cancels on her 'cause he says he's hanging with his friends. She can't believe he'd rather run with the boys than give her nice big tits some love, so she dumps his ass and turns her attention to the neighbor, Ryan, who's doing some work around the property for the landlord. She reels him in, away from his raking, and gets him to agree to some skinny-dipping. But fuck that, she says, she'd rather just get naked and fuck! So they do.
Deleted due DMCA report


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Manila Clams with Lentils and Kale
Small, sweet Manila clams are a fixture on the menu at Renee Erickson's Seattle oyster bar, The Walrus and the Carpenter. To make a meal of them at home, she adds French green lentils and kale. The clam juices, white wine and crème fraîche create a magnificent broth that's delicious with grilled bread for dunking.
Recipe from Food & Wine Chefs' Easy Weeknight Dinners.
- Active:
- Total Time:
- Servings: 2 to 4
- 1 cup French green (Le Puy) lentils, picked over and rinsed
- 1 garlic clove, smashed
- 1 fresh or dried bay leaf
- 3 tablespoons unsalted butter
- 2 shallots, thinly sliced
- 1 tablespoon thyme leaves
- 3 pounds Manila clams or small littleneck clams, scrubbed
- 3 cups white wine
- 1 cup crème fraîche
- Thinly sliced zest of 1 lemon
- 3 tablespoons fresh lemon juice
- Salt
- 1 bunch of Tuscan kale, stems trimmed and leaves chopped
How to make this recipe
In a medium saucepan, combine the lentils, garlic, bay leaf and enough cold water to cover the lentils by 2 inches. Bring to a boil over high heat, then reduce the heat to moderately low, cover and simmer for 30 minutes, until the lentils are tender. Drain well; discard the garlic and bay leaf.
In a large saucepan, melt the butter over moderate heat. Add the shallots and thyme and cook, stirring, for 2 minutes. Stir in the clams and wine, cover and cook over high heat, shaking the pan occasionally, until the clams open, 6 to 8 minutes. As the clams open, transfer them to a bowl; discard any clams that do not open.
Stir the crème fraîche, lemon zest and lemon juice into the large saucepan and season with salt. Add the kale and lentils and cook, stirring, until the kale wilts, about 1 minute. Return the clams to the saucepan and stir to combine. Serve the stew in deep bowls.
Serve With
Grilled bread and lemon wedges.


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A serene accommodation palace one of Limassol's oldest establishments, the Curium Palace carries years of colonial history aristocratic flairs and local culture. Newly refurbished, the Curium Palace continues in presenting it's well established history in a modern but classical manner. There are 66 standard rooms, 2 triple rooms, 3 interconnected rooms, 3 Junior Suites and 3 Executive Suites. Standard rooms have twin or double bed. Junior Suites has a seating area. Executive Suites have separate seating and t.v. area.
Distance to town center: 1 kmDistance to Larnaca Airport: 75 kmTransfer time to/from Airport: 45 minutesDistance to Paphos Airport: 75 kmTransfer time to/from Airport: 45 minutes


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A modern recycling plant has been setup in Newport that's completely equipped with advanced technology. It was opened by the First Minister Rhodri Morgan who said "Newport will become one of the flagships for the recycling industry". He praised Sims Recycling Solutions for its effort in recycling IT products. This plant is capable of recycling half a million tonnes of electronic waste every year.
Apart from this, the plant will eliminate and process the toxic elements present in the materials and cut up metals in the recycling process.
There is also a continuous demand for recycling old mobile phones. Sims Recycling Solutions Chief Graham Davy said:"We are happy about this modern plant as it will meet the demands of the UK Environment Agency for recycling in the longer term and is good news for people in Newport. It's one of the largest companies in recycling electrical and electronic goods across the globe.


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Competitions RWS Contemporary Watercolour Competition 2017
NOTE: This opp listing has expired and may no longer be relevant!
Opportunity Description
The Royal Watercolour Society (RWS) was founded in 1804 and is the oldest and most prestigious watercolour society in the world. Past Members include Sir Lawrence Alma-Tadema, E.R. Hughes, William Hunt, Henry Moore and John Singer Sargent.
From our home at Bankside Gallery, situated on the vibrant south bank next door to Tate Modern, we hold regular exhibitions showcasing the best in water-based media. The Society also hosts talks, watercolour courses, family art events, open competition and art demonstrations.
Royal Watercolour Society
Bankside Gallery
48 Hopton Street
t: 020 7928 7521
* The call for artists from this organization has expired. Please see other opportunities on ArtDeadline.Com.
About ArtDeadline.Com
Artdeadline.Com was the art worlds first and still most respected resource of its kind. Selected by 1000's of artists, fine art galleries, museums, art agencies, and educational institutions for both news distribution and art resources since 1994.
We receive and publish more opportunities and event listings per day than any other online or print publication, much of which is exclusive content. We are the leader in the publication of:
- Art Competitions
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|Re-enactment at Oliver Café in Jakarta, where the victim died after sipping
an iced coffee her friend had bought for her.
The family of Wayan Mirna Salihin, the former Sydney design student allegedly poisoned at a Jakarta cafe in January, has lambasted the 20-year jail sentence sought by prosecutors saying the Indonesian-Australian woman accused of her murder deserved the death penalty.
Prosecutors late on Wednesday demanded that the Central Jakarta District Court find Australian permanent resident Jessica Kumala Wongso, 27, guilty of premeditated murder.
Salihin, who met Ms Wongso while studying at Billie Blue College in Sydney, died on January 6 after sipping an iced coffee her friend had bought for her.
"It can be concluded that the defendant has laced with cyanide the coffee drunk by the victim when the coffee was in the defendant's possession," prosecutor Melanie Wuwung told the court.
Although the maximum sentence for premeditated murder is the death penalty, Indonesia has promised it would be taken off the table after requesting assistance from the Australian Federal Police in the case.
Ministerial approval is a necessary condition for Australian assistance in investigating crimes that could lead to the death penalty, after the AFP tip-off that ultimately led to the execution last year of Bali Nine ringleaders Andrew Chan and Myuran Sukumaran.
Lead prosecutor Ardito Muwardi said a 20-year-prison sentence fitted the crime.
"A sentence demand depends on the prosecution's subjectivity. Subjectively, we feel that 20 years is enough," Mr Muwardi told television station MetroTV.
Salihin's widower, Arief Sumarko, was furious.
"We were newlyweds. We were in love. She is my closest friend. We were going to start a family. But she was taken from me," Mr Sumarko told The Australian.
"How can I accept? She doesn't deserve this. We all know who killed Mirna. I hope the judges sentence Wongso as severely as legally possible."
Ms Wongso's lawyer, Otto Hasibuan, said the defendant "does not deserve a day in jail".
Mr Hasibuan highlighted testimonies by Australian toxicologists led by Beng Beng Ong, who claimed there was insufficient evidence to conclude that Salihin died of cyanide poisoning. saying that "death of natural causes cannot be ruled out".
Prosecutors told the three judges to ignore the defence experts' because their credibility and integrity were questionable.
The defence is scheduled to provide a counter argument next week.
Source: The Australian, October 7, 2016
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Thanksgiving was such a blast. The turkey was moist, the dressing was flavorful, and good times were had by all. We ate until we were stuffed and we played games late into the night.
Now that Thanksgiving is over the next thing to look forward to is Christmas! Here are some Friday Favorites that will make the holiday even brighter.
This A.B.S by Allen Schwartz Sleeveless Studded Sheath Dress is a great mix of naughty and nice. The studs give the dress a flair over just a simple Little Black Dress.
I've never made brittle before, but my mom always made peanut brittle for Christmas. This recipe for Salted Pistachio Brittle is an interesting take on brittle.
Nonstick vegetable spray
1 cup sugar
1/2 cup light corn syrup
1 cup raw, unsalted pistachios coarsely chopped
1 tbsp unsalted butter
1 tsp kosher salt
3/4 tsp baking soda
Course sea salt
Line a rimmed baking sheet with parchment paper; spray with nonstick spray and set aside. Whisk sugar, corn syrup, and 3 tablespoons water in a medium saucepan. Stir over medium heat until sugar dissolves. Fit saucepan with candy thermometer, bring mixture to a boil, and cook until thermometer registers 290°F, 3-4 minutes.
Using a heatproof spatula, stir in pistachios, butter, and kosher salt (syrup will seize initially, but will melt as it heats back up). Continue to cook syrup, stirring often, until thermometer registers 300°F and pistachios are golden brown, 3-4 minutes. Caramel should be pale brown (it will darken slightly as it cools). Sprinkle baking soda over and stir quickly to blend caramel thoroughly (mixture will bubble vigorously).
Immediately pour caramel onto prepared baking sheet and, using a heatproof spatula, quickly spread out as thin as possible. Sprinkle sea salt over and let caramel cool completely. Break brittle into pieces.
Normally when I am invited to dinner I like to bring a small gift for the hostess. This Furoshiki Gift Wrapping puts a special touch on the average bottle of wine.


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USFFC 20: Carroll vs Moon featuring The Rory Kelly Band
USFFC 20: Carroll vs Moon featuring The Rory Kelly Band - Mixed Martial Arts (MMA) Event on September 20, 2014 in Morganton, North Carolina USA
USFFC 20 "The Reckoning" is the first event to take place in Morganton, North Carolina. This is event is a pro/am event. Tickets will be on sale July 1st, 2014 at shop.battleforged.com
Directions: The Old Armory Event Center is a beautiful remodeled Armory from the Pre-WW2 Era! It is located less than 2 miles off I-40 in Western North Carolina. Take exit 105 and travel towards the downtown area.


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$595 retail
or 4 payments of
At your door
by 8pm
Drop in mail
by 5pm
Known for their coveted rustic florals, Zimmermann makes a comeback this season with the Lavish playsuit. Crafted out of soft silk georgette with illustrative details, it features unlined flared bell sleeves for the perfect feminine statement. Backless with a relaxed fit, the high neckline balances out the silhouette beautifully. Rent this for an outdoor wedding or a special dinner date!
This playsuit looks amazing on all body types. Wear your hair up and hire gold jewellery to match the tones of this incredible print! Keep styling minimal to add sophistication to the bohemian trend. Versatile for both day and night – read our blog post on how to rock the playsuit this spring!
About the designer
Zimmermann has enjoyed success as one of Australia's eminent fashion labels since their beginning in 1991. Started by sisters Nicky and Simone Zimmermann, the label encapsulates the classic Australian woman - laidback yet stylish, merging beautiful, delicate fabrics with unique, savvy cuts. Their dresses range from the feminine and flirty to the sleek and fashion-forward, making them perfect for a variety of events. GlamCorner allows you to hire Zimmermann dresses at a fraction of their retail price - these stunning dresses can now be your go-to for summer events and functions!
All dresses listed are in Australian sizes. Model wears a size 8.
BUST: Slightly relaxed fit. Refer to the size guide for specific measurements.
WAIST: Fitted, but elasticated with adjustable tie.
HIPS: Relaxed fit as the shorts flare out.
LENGTH: This playsuit is mini length. Please note that our model is 5'9".
UNDERGARMENTS: As the bodice is backless, consider wearing silicone cups or going without a bra to avoid any visible lines.
FABRIC: Fabric does not stretch.
GREAT FOR: Pear, Hourglass, Curvy, Straight, Narrow body types.
Since sizing and cut varies between dresses and brands, please use this chart as a general guide. The sizing in this chart applies to this dress ONLY. If you need assistance selecting the proper size, please contact our friendly customer service team.
Estimated standard delivery time is 1-2 business days - depending on your location & the time you submitted your order.We recommend you select your delivery date 1-2 days before your event to make sure everything's perfect.Same-day delivery is available in Sydney Metro area.If you place an order on a weekend or public holiday, your order will be processed on the next business day.Standard delivery fee is $9.95 for all rentals except Try On orders. Try On orders are free of shipping cost.
Returning your rental item is very simple and free. Simply place your item in the pre-paid return bag provided by GC, and drop it at your nearest Post Office or the yellow Express Postal Box.If your rental period ends on a Sunday or a public holiday, just drop your item in the nearest yellow Express Postal Box on the return date. It will be shipped back to us in the next business day.We take care of dry-cleaning too so you don't have to worry about it.
Late returns
In the unlikely event that you returned your rental item back late, you will incur late fees.Please see FAQs for more details.
You can book up to two dresses to try on within the convenience of your home for $30. For more information on our Try On service, please visit this link.
Try On service is only available for dresses. Please note that we are unable to hold dresses for your actual event date until a booking order is placed.
Join GlamCorner now to get
all the latest looks and style tips


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- Limited Edition - SevenFriday Watch Woody P2B/03 Limited Edition D (SVF-019)
Official Authorised Agent
Limited Edition of 450 pieces. SOLD OUT
This addition to the Industrial Revolution line is inspired by historic industrial production methods, hand hammered copper, old iron tools and the art of wood joinery. This is the first SEVENFRIDAY to feature Super Luminova on the hour hand and on the 12 different hour indicators, making it easy to read the time in low light. This watch is also the first model outside the V-series to feature our new NFC CP mechanism. The highlight of this watch is the wooden animation ring. The P2B/03-W box is surrounded by an animation ring made from seven layers of wood. American walnut, beech and solid cherry timbers were cut, shaped, individually lacquered, layered and then bonded together by master craftsmen at Watschinger, an Italian luxury wood joiner with over 60 years crafting experience. P2B/03-W
Buy your new watch from C W Sellors - Jura Watches.
Try it on at home
If you do not like it, you can return it for an exchange, credit or full refund.
Let us adjust your bracelet
We can adjust the metal bracelet to fit your wrist size, see our size guide
Get free delivery
Buy online and we will ship your watch free and ready to use, fully insured by us and delivered to your door.
Free Gift
Watch Winder or Watch toolkit or heart necklet plus free delivery, sizing and gift wrapping.
Accessories for this watch
Please contact us if you need any straps for this watch.


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WebShot ~Will take a screenshot of the browser easily~
13 декември 2015, 19:06
About WebShot ~Will take a screenshot of the browser easily~
This app is an app to take a screenshot of the browser.
The Thats caught in iPhone functions normally! Anyone who thinks I! !
Is a big mistake! ! !
In fact, I take screenshots of the entire screen of the site!
If it is function of the iPhone, and I have to take as many times as you move, but only one-tap if this app! ! !
Please taste once its great features!
Perhaps, we are hooked! ! !
Simmilar Apps
16.12 10:49
01.12 19:52


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Fresh White with Granite Accents
3 Fresh White Galvanized Steel Exclusive blades included
Worlds collide in exciting ways with the Duluth, an industrial fan that's fit for your home. This product boasts purely industrial aesthetics from top to bottom - from the prominent rivets securing the motor to the turbine-inspired switch housing cap. It's like a piece of modern machinery walked right into your room, yet the fan carries a certain grace and ease that allows it to blend right in to any loft or contemporary environment.
Install the new Casablanca Fan Visualizer App and get fresh inspiration for your space.
7988 cfm
Electricity Use
112 watts
Airflow Efficiency
71 cfm/watt
Model 59192
5-star 1 review
Recommended by 100% of reviewers
August 5, 2016
eric m.
The Duluth is a beautiful fan. It finishes off a perfect room.
August 25, 2016
We're happy to hear you're enjoying your new Duluth, Eric! Welcome to the Casablanca family!


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Haragei was the Rokugani term for trusted instinct. When the belly could perceive things the mind could not. It could tell how to act, even before the threat had appeared. This instinct was trusted by many samurai. [1]
Sparrow Clan Edit
Haragei was Suzume's philosophy of warriors. To be as the sparrow, to trust one's belly, to allow one's instincts to warn one of danger. It was the first lesson of the Suzume School of Swordmanship, to recognize danger by instinct, to perceive an action before it has occurred, and to act without hesitation. [2] Literally translated, the term meant "stomach art", and it referred to a exchange of thoughts and feelings that was implied in conversation, rather than explicitly stated.
- ↑ Trusted Instinct (Torn Asunder flavor)
- ↑ The First Lesson, by Robert Denton
|This article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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Frog is a white street-level dealer whose allegiances are never made clear. He is seen early in season two distributing a package for Ziggy Sobotka (and stealing from him in the process), and later negotiates with Nick Sobotka to distribute another. During the early stages of season two's investigation, he sells heroin to Thomas "Herc" Hauk and is photographed by Kima Greggs and Ellis Carver.
- Played by: Gary 'D. Reign' Senkus
- Appears in season two: "Undertow"; "Backwash", "Port in a Storm" (uncredited) and -30- (uncredited).


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At about 1715z I QSYed to 10m JT65 from 6m JT65. I may have left it too late as I have not had any spots on 10m yet. Maybe, there was no European propagation to link to the TEP area? Anyway, I'll leave things running for a few more hours.
UPDATE 1927z: IZ4RE (1138km) spotted me in the last ten minutes.
UPDATE 2200z: I finally went QRT at around 2145z. In all, 5 South Americans were spotted on 10m JT65, all after dark here. Best DX on 10m JT65 RX was Argentina. I was not expecting to spot any South Americans on 10m this late. Surprised again!
31 Oct 2016
QSYed to 10m JT65
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Post your job for free and up to 3 tradesmen will contact you to quote
This page has everything you'll need to know about finding and hiring a local handyman. Learn about this trade and how they can help you around the home.
Take a look at our Handymen profiles, read reviews and request quotes directly.
Handyman in Renfrewshire
We offer a complete service from joinery to electrical all services are catered for . We offer a quality service at reasonable prices with a full guarantee and free quotations
Trades serviced: Home improvements, Loft Conversion, Wooden Doors - Internal, Laminate Flooring, Solid wood flooring, Fitted bedrooms / Wardrobes
Latest homeowner review
very prompt, quickly identified the problem and resolved it at a resonable price. did other checks too, overall definate recommend him....
Handyman in Renfrewshire
Trades serviced: Garden Wall, Chimney Building / Repair, Pointing / Repointing, Stonework / Stone Cladding, Cellar & Basement Conversion, Conservatory
Latest homeowner review
Excellent job and great value for money.
Handyman in Renfrewshire
Trades serviced: Bathroom Design, Bathroom Installation, Complete Bathroom Refurbishment, Garden Wall, Chimney Building / Repair, Pointing / Repointing
Handyman in Renfrewshire
Trades serviced: Chimney Building / Repair, Cellar & Basement Conversion, Conservatory, Garage Conversion, House extension, Home improvements
Latest homeowner review
Very helpful and committed to do the work another day and returned as promised to carry out the work in a professional manner
Handyman in Renfrewshire
Trades serviced: Whole Internal Refurbishment, Internal Painting & Decorating, Carpet fitters, Laminate Flooring, Linoleum Flooring, General Fitted Furniture
Handyman in Renfrewshire
Trades serviced: Bespoke kitchens, Kitchen design / Installation, Fitted kitchens, Complete Kitchen Refurbishment, Laminate / Wooden kitchen worktops, Kitchen Worktops - Stone
Latest homeowner review
william barker got in touch very soon after me posting the job and had me booked in with the time frame i was wanting,excellent job from start to finish. First class job many thanks would highly recommend
Handyman in Renfrewshire
Trades serviced: Cellar & Basement Conversion, Conservatory, Garage Conversion, House extension, Home improvements, Loft Conversion
Handyman in Renfrewshire
Trades serviced: Wooden Doors - Internal, Wooden Shutter, Bespoke furniture, Fitted bedrooms / Wardrobes, General Fitted Furniture, Bespoke kitchens
Handyman in Renfrewshire
Windows ,Doors and Conservatories. Extensions, Garage conversions. Most Joinery and building work undertaken. Bespoke furniture made to order, garden furniture and landscaping.
Trades serviced: Garden Wall, Pointing / Repointing, Stonework / Stone Cladding, Cellar & Basement Conversion, Conservatory, Garage Conversion
Latest homeowner review
Job done well , on time and no extra costs - very satisfied
Handyman in Renfrewshire
Trades serviced: Garage Conversion, Wooden staircases, Wooden Doors - Internal, Floor Sanding & Finishing, Laminate Flooring, Solid wood flooring
Latest homeowner review
Excellent from start to finish. Was really helpful, punctual, good work and very happy with price. Will be using again when work needed.
Create a job - it's free
We'll connect you with the right tradesmen, from over 30 different trades.
Get quotes
Up to 3 tradesmen can provide a quote, but on average you'll get 1-2.
Check ratings
Look at each tradesperson's profile to see their ratings and reviews.
Pick a tradesperson
Use our homeowner checklist for extra tips on choosing a tradesperson.
Job done
Leave a rating for your tradesperson based on quality, reliablity and value.


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TripAdvisor 2015
Travel + Leisure 2016
Plan Your Trip
Get Started Now!
Quick links to help you plan your perfect trip
Getting here is half the fun and there are so many easy options to choose from:
- Washington State Ferries: A leisurely ride on a Washington State Ferry from Anacortes gives you a taste of the archipelago's beauty, with a choice of stops at four islands—Lopez, Orcas, Shaw or San Juan. You can now make a reservation at www.takeaferry.com. Ferries will accommodate cars as well as walk-on passengers.
- If you plan on traveling to the islands over the next few weeks (July/August 2017), make sure to check the ferry alerts page for updates: www.wsdot.wa.gov/ferries/schedule/bulletin.aspx.
- Seasonal Passenger Ferries: From Seattle's waterfront, Port Townsend or Bellingham, hop onto the San Juan Clipper, Puget Sound Express, or San Juan Cruises (respectively) for a day or overnight trip to Friday Harbor on San Juan Island.
- Air Travel: Fly from Seattle or SeaTac International Airport on a Kenmore Air seaplane or wheeled-plane, or take a regional flight.
- Car Rental: Rent a car at SeaTac and drive to the Washington State Ferry terminal in Anacortes.
- Airport Shuttles: Take an Airporter Shuttle from SeaTac to the Washington State Ferry terminal in Anacortes.
- Visitor Information Centers: When you arrive in the islands, stop in at the Orcas, Lopez and San Juan Island Chambers of Commerce for maps, brochures and information about where to go and what to do during your stay.
- Getting Around: When you get to the islands, you can also get around with shuttles, mopeds, bikes, and car rentals.
San Juan Islands Events
Featured Event
Thursday - Monday, May 26 - Sept. 4
Emergence: First Nation Legendary & Emerging Artists
Upcoming Events
- August 12, Lopez Island
September 22 to November 11, Lopez Island•Orcas Island•San Juan Island•Friday Harbor
Explore the Islands
Featured Experience
10 Fun Things to do this Summer in the San Juans
Long, sunny days let you love your endless summer in Friday Harbor, San Juan Island, and on Lopez and Orcas Islands
Dining in the Islands
Fine dining in the San Juan Islands means tasty food—often locally produced—served in a laid-back atmosphere (forget the jacket and tie). Many of the islands' restaurants take advantage of the bounty from local growers. Consider adding a local wine, beer, cider or spirit to your meal in these establishments—labels from San Juan Vineyards, Orcas Island Winery and Lopez Island Vineyards & Winery are featured on many of their wine lists.


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My family and I, come to Keystone every year for our vacation. We normally stay at the Evergreen Condominiums. We booked kind of late and were not able to get into that Condo. So we decided to stay here. The prices are fairly cheaper than where we normally stay, but definitely not as nice. They are smaller, and the one we stayed in was a two bedroom plus loft. The loft was the size of a very very small bedroom, and the stairs were not the best. The condo is very out dated, 1980's look, and the windows don't open. On a brighter note; they were very clean and the staff of course is still very friendly. My kids did enjoy having an outdoor pool and hot tub. The location is great as far as it's not to far from the ski resort, and not to far from the town.
- Reservation Options:
- TripAdvisor is proud to partner with Orbitz, Expedia, Hotels.com, Travelocity, Hotwire, Cancelon, HotelQuickly and getaroom.com so you can book your Forest Condominiums reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
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In the eyes of the MSM, Pope Benedict XVI has a problem. He's too darn . . . Catholic.
As the Pope begins World Youth Day in his native Germany, Today took the occasion to invite in Newsweek's Christopher Dickey for an oh-so-effete critique of Benedict XVI.
Interviewed by Katie Couric, Dickey first claimed that Europeans view Benedict "with some skepticism right now. People are looking at this Pope and saying what does he want to do by re-Christianizing Europe?"
Gee, and here I thought that was a Pope's job.
Dickey then added, with breathtaking condescension, "on the other hand he isn't creating the kinds of problems people thought he would. There was a lot of suspicion that he would be so emphasizing Christianity that it would alienate Muslims, that it would alienate Jews in Europe."
What a relief. We certainly can't have a Pope creating problems with that Christianity stuff.
Dickey let us know that things are no better in America. "I think American Catholics are worried" about the Pope, confessed Dickey with a sigh.
Why? "Because his record as the guardian of doctrinal purity for the last 20-something years was a record of great conservatism. He certainly was hostile to the idea of the ordination of women. There certianly is not going to be any movement on the issue of abortion."
Continued Dickey: "the issue of right to life generally from conception to death is one that is very important to him and to the extent that runs up against modern American values even among modern American Catholics, that suggests there will be confrontation."
Apparently there are no traditional Catholics left in the USA - only "modern" ones, in tune with "modern American values," who disdain "conservatism" and clamor for women priests and abortion on demand.
So here was one MSM standard-bearer interviewing a representative of another MSM mainstay, and the consensus was clear: among right-thinking people of sophistication, this Pope is really something of a bother and an embarrassment with all his unseemly emphasis on Christianity. It's really time to get modern, don't you know?


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How to Create a Nutcracker makeup look for the holidays
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It's all about perspective. A winning job resume is your personal marketing tool, and you have to use it properly. Think always of your resume as a selling tool that outlines your skills and experiences so that an employer can see, at a glance, how you can contribute to the company.
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Presenters, Performers, Lecturers, Entertainers
Presenters, Performers, Lecturers, Entertainers
Adult, Teen and Youth Audiences
The Geneva Public Library District serves a population of 30,000+ residents. We have one Meeting Room with a 150 person capacity in which we provide a wide-range of special programs to meet the diverse needs of our patrons. Thus, it experiences a very high usage rate.
You may use this form to propose your program to us and it will be routed to the appropriate staff person in charge of that particular audience. Please note: You will receive a response from us only if we can accommodate your program.


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Saladin Ahmed on Islamic Folklore
Over at Fantasy Magazine, Saladin Ahmed talks about The Messengers, Monsters, and Moral Instructors of Islamic Literature:
The lore of every culture in human history contains sentient beings that are more than or less than human. Elves and vampires, nagas and orishas, daemons and spirit animals—each of these creatures has powers beyond those of mortal men and women, and each is enmeshed in the moral and symbolic order of creation particular to the culture from which it arises. Their strange forms and powers, their familiar-yet-foreign drives and weaknesses, teach humans about what it means to live in this world and the worlds beyond.
The stories and scriptures of the world's numerous Muslim cultures are no different. Islam's holy book (the Qur'an), its contested doctrinal traditions (the Hadith), and its various folklores are brimming with powerful nonhuman creatures.
First of all, there is the constitutive presence of God Himself in these texts and traditions. Below the divine, there are also several lower orders of nonhuman beings present. Angel, Djinn, and Ghoul: each of these creatures appears in numerous Islamic scriptures or stories, from theQur'an to the Thousand and One Nights to epic poetry. The cultures of Islam are profoundly diverse, of course, spanning centuries and continents. Local iterations of these beings reflect this diversity. But in every instance, when humans encounter these creatures, it is a test of faith, wit, and bravery. – continue reading.
2 Comments
Got to hear Saladin Ahmed read an excerpt from his novel at Worldcon: it featired a ghoul hunter, his Dervish warrior assistant, rock ghouls, and a magic system that uses quotes from the Qur'an. Very cool. Also saw him on a panel about non-European fantasy elements. I think that many of th
Comment by amy west | August 24, 2011
e interviews in Fantasy Mgazine are useless, but this one might be worthwhile.
Comment by amy west | August 24, 2011
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text: MetLife, Inc. (NYSE: MET), Bank for Investment & Development of Vietnam (BIDV), and Bank for Investment and Development of Vietnam Insurance Corporation (BIC) signed an agreement today to establish a life insurance joint venture (JV) in Vietnam. The signing ceremony was witnessed by Prime Minister Nguyen Tan Dung of Vietnam, Tran Bac Ha, chairman of the board of management of BIDV, and Kurt Campbell, member of MetLife's board of directors and former assistant secretary of state, during the prime minister's visit to the United States. The JV follows the Memorandum of Understanding (MOU) that was previously signed by both parties in July of this year and delivers on a key component of MetLife's strategy: expanding its presence in emerging markets. At the same time, the JV also represents BIDV's strategic direction into the field of life insurance, which is in line with and complements the bank's business tradition. The new JV will have a charter capital of VND 1 trillion (approximately US$48 million), and MetLife will own 60% of the company and BIDV and BIC will have the remaining 40%. The joint venture will initially focus on offering life and health insurance products. Alongside the establishment of the JV, there is also an exclusive bancassurance distribution agreement between the JV and BIDV. The JV, which is expected to be operational in 2014, will leverage the strengths of two exceptional companies to create a strong and trusted insurance provider. Vietnamese consumers will benefit from access to MetLife's global expertise, financial reliability and a wide range of innovative products and services. In addition, the JV will also leverage BIDV's keen understanding of the financial needs of Vietnamese customers and its large national branch network of more than 600 distribution points. Commenting on the signing, Chris Townsend, president, Asia for MetLife said, "This JV agreement between MetLife and BIDV expands MetLife's footprint into fast growing Southeast Asia and highlights our commitment to the country. We look forward to partnering with BIDV, a bank with a long and distinguished history and brand, to deliver a suite of innovative life insurance products to customers in Vietnam in order to provide them with the ability to achieve and maintain their financial security."


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If you are in need of emergency food assistance, contact one of our partner agencies in Lumpkin County. Each agency has different hours of operation and requirements. Therefore, we suggest that you call the agency for specific information. If you are unable to find help near you, please contact Lydia Johnson at 770-534-4111 or via email.
Community Helping Place Inc
1127 Georgia 52
Dahlonega, GA 30533
706-867-9621 x 309
Day / Time: Monday, Wednesday & Friday 10am – 1pm
Notes: 1st visit: photo ID, Lumpkin County Resident, Ongoing: photo ID, Lumpkin County Resident, 150 % of poverty guidelines.
North Central / There's Hope for the Hungry
1230 Samples Industrial
Cumming, GA 30041
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523 Ben Higgins Road
Dahlonega, GA 30533
Day / Time: Second Saturday 10am – 1pm
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Unseen Hands
4632 Auraria Road
Dawsonville, GA 30534
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The Waypoint Center – Dahlonega
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Copyright © 2016 Georgia Mountain Food Bank Website by Forum Communications


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Suspect arrested in connection with brutal gangland shooting
Detectives involved in a long-running investigation into a Crumlin/Drimnagh feud murder have arrested a key suspect in the case.
The Dubliner, who is aged in his 30s, is part of the 'Fat' Freddie Thompson mob and was grilled by gardai over a number of days about his suspected role as a "scout" in the shocking murder of Gerard Eglington (27), who was shot dead in Portarlington, Co Laois, in September, 2012.
Eglington was killed because he was heavily-involved in Ireland's deadliest drugs feud - the bitter gang war between Thompson's gang and a crew who were allied to jailed gangland killer Brian Rattigan.
The suspect had fled to France after the murder and was picked up by gardai on his return.
Officers arrested the suspect after fingerprint evidence showed up on a car that was seized after the brutal gangland slaying. The car had been seen staking out the house before the murder happened.
"The mob who carried out this murder had used individuals to travel in cars to scout out the area before Eglington was shot dead," a source said last night.
"That is what this man is suspected of doing, and while there were a number of arrests in the aftermath of the murder, there is expected to be more in the coming months," the source added.
The arrested man, who has previous convictions, was released without charge and a file is being prepared for the DPP.
Eglington was at home with his 11-year-old stepdaughter and three-year-old son when the attack occurred at about 8.30am on September 24, 2012, at Kilnacourt Woods, Portarlington.
The slain gangster was previously involved in a savage assault on the brother of exiled gangster 'Fat' Freddie Thompson at the Karma Stone pub in the city centre on March 13, 2011.
Via Herald.ie


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Nissan Prices and Values: New Nissan models include the Altima, Maxima, Armada, Murano, Titan, Pathfinder and Sentra. Nissan SUVs also include the Xterra and CrossCabriolet. Base prices, for models such as the Pathfinder, may appear surprisingly affordable. Used Nissan values vary by model, while the Leaf's book value is subject to frequent updates. Nissan deals change monthly.
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Select a used 2001 Nissan Sedan model
(2018 - 1998)
2001 Nissan Altima
The 2001 Nissan Altima is a stylish and fuel-efficient compact sedan that is rated at over 30 mpg on the highway.
2001 Nissan Maxima
The 2001 Nissan Maxima is a stylish and sporty mid-size sedan with seating for five passengers and a 222 hp V6 engine under the hood.
2001 Nissan Sentra
The 2001 Nissan Sentra is a compact five-passenger sedan which offers sporty styling and a fuel-efficient ride with a highway fuel economy rating well over 30 mpg.


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Alison Cooley | A New Series & Studio Visit
We're excited to launch a new series of gorgeous paintings by artist Alison Cooley.
We got some insight into Alison's practice, what influences her and a little context into the intriguing title behind the new series "chirographs." Read our interview with Alison and explore her new paintings.
We're excited about this new series of paintings! Tell us a little bit about these new works you've been creating, what's the story?
I've always been attracted to drawing and marking in painting. In my latest work, I'm creating a kind of written visual language that's my own but is open to interpretation by the viewer. It's like a "secret language" that I create in line, form and tone that I want people to read in their own way.
I've been looking at artists who use molten glass on paper (pyrography) and at the same time realized I was making my own "ancient language." I love the sound of the word "chirograph" which refers to the study of writing by hand (chiro-) and also to medieval contracts. My own chirographs are language markings that are a two-way contract with the viewer.
Last gallery show you went to?
"Unfinished" at the Met Breuer.
Tell us about the best studio visit you've had.
In London an art history professor came to my studio and said "You don't know what you're painting now but you will in the future." I wasn't sure what he meant at the time, but I have a better idea now. It's the esoteric idea that you know your future self now. Painting is my way of arriving at a place where I already am.
Have the cities you've lived in influenced your practice? If so, tell us a bit about that, and what elements in particular steered you in certain directions.
I think my painting is a mirror to a lot of issues that I experience navigating the world. I'm very tactile, and also am hyper-sensitive to texture and smell. Living in London, I had this explosion of awareness of what you might call "personal climate" - the aura and weather of people's skin and how everyone has an original atmosphere around them, like a fingerprint. I grew up in Washington, DC and have indelible memories of the dragging, almost New Orleans-like murk of summer weather. On Nantucket, I was like a bird in the wild, more expansive but equally tuned in to shifting air, light, and sea. Now I think I'm merging all of these experiences into my own language, and being much more ok with letting it be my own, not "translated."
Best gift you've ever received?
My child.
What is your favorite quote?
"All my smooth body" - the ghost in Hamlet.
What country do you wish to visit?
What makes you laugh no matter what?
Keyboard Cat
Whats the first thing you do when you begin formulating an idea for a painting?
Build a new playlist to create a gateway. Since I often work in a series, I edit and change the playlist as I move from one piece to the next. In a way I am developing a soundtrack to my process, an audible experience that echoes and inspires the work.
What work took you the longest to complete?
In this series, Chirograph 6.
Tell us about some of your favorite artists.
Recently I saw Cecily Brown speak at the National Gallery of Art and I loved hearing about how referencing the past informs her paintings. Her sources included a wide reaching range of artists including Goya, Hogarth, Bruegel, among others. Continuity is a such a big theme for me and "quoting" from the past is an intriguing and rich part of process. I draw from so many different places — Nathan Oliveira, John Singer Sargent, Bernini, Miro.
What's one habit you wish you could break.
Meandering through Pinterest before I go to sleep.
Who was your favorite teacher in art school?
Eve Aschheim at Sarah Lawrence.
What does creativity mean to you?
Being radically open to your senses and experiences.
What's something you can't do?
It's weird, but I am awful at opening small packages so I have dozens of pairs of scissors around my studio and kitchen to snip open bags, boxes and cases. I'll even cut an avocado with scissors.
What's the most adventurous thing you've done?
Pack up my family and move overseas.
What's one thing you've always wanted to try but you've been too scared to do?
Take flying lessons.
What else are you working on right now?
A series of work on handmade Turner grey paper and some large drawings on lacquered board.
When do you make your best work?
In the morning after a long walk outside.
Regarding your method of making, is it a case of the material or method dictating the idea of the other way around?
Method…I cycle through materials — pens, ink, oil sticks — I love, devour, and learn from my materials and move on to the next.
Is art making therapeutic for you?
Yes, It's essential for me and a key part of how I experience the world.
What are you most proud of?
Always finding the discipline and space to paint….even in really, really, really small apartments.
What do you see yourself doing in 5 years?
Painting gigantic, expansive paintings that allow my language to tell multiple tales.
If you could travel anywhere to create for a while, where would you go?
St. Petersburg, Russia.
Did you grow up around other creative people?
Yes, I grew up in Washington DC and we spent a lot of time in museums as kids. Maybe more importantly, I am from a family of voracious readers, so I was introduced to the transformative experience of losing yourself in a book — someone else's world and language — at an early age. My family joke about book hangovers — the feeling that once you finish a truly moving book, you can't shake it for a few days.
What are your other hobbies?
Yoga, reading, music.
What motivates you?
The fact that as an artist you can live in a magical reality for the hours you are working and creating.
What's your studio philosophy?
Just show up and work.
How many hours do you try and work in the studio per week?
30-40 hrs.
Silence or sound while creating? If sound, what?
Music is intertwined with my work, when I'm not painting, I am engaged in making playlists and discovering music that might take me on a new voyage. Back in the day, I made some masterpiece mix tapes which I still have even though I don't have a tape player.
Tools or mediums you're dying to experiment with?
Heat or wax.
Any advice to aspiring artists?
You are your best self and worst enemy in the studio…find the space right on the edge and go with it.
What's next?
We'll see!


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Italian Society of Bioinformatics (BITS): Annual Meeting 2011
Eighth Annual Meeting of the Italian Society of Bioinformatics (BITS)
MicroRNAs (miRNAs) are small RNAs (21-24 bp) providing an RNA-based system of gene regulation highly conserved in plants and animals. In plants, miRNAs control mRNA degradation or restrain translation, affecting development and responses to stresses. Plant miRNAs show imperfect but extensive complementarity to mRNA targets, making their computational prediction possible, useful when data mining is applied on different species. In this study we used a comparative approach to identify both miRNAs and their targets, in artichoke and safflower.
Two complete expressed sequence tags (ESTs) datasets from artichoke (3.6·104 entries) and safflower (4.2·104), were analysed with a bioinformatic pipeline and
Mature miRNAs and targets were predicted within EST sequences of safflower and artichoke. Most of the miRNA targets appeared highly/moderately conserved, highlighting an important and conserved function. In this study we introduce a stringent parameter for the comparative sequence analysis, represented by the identification of the same target in the COS region. After statistical analysis 79 targets, found on the COS regions and belonging to 60 miRNA families, have a signal to noise ratio > 2, with ≥ 0.85 specificity. The putative miRNAs identified belong to 55 dicotyledon plants and to 24 families only in monocotyledon.
The family
Significant progress has been achieved in recent years on the mechanisms of gene regulation and expression in plant and animals
A class of tiny RNA molecules,
The biogenesis of miRNAs in plant is a multi step process: initially a miRNA gene is transcribed in the nucleus (pri-miRNA) by means of POLII enzyme
Unlike animal mRNA targets, plant targets show a single sequence motif displaying a near-perfect complementarity to their miRNAs. The imperfect but extensive correspondence of plant miRNAs to their mRNA targets provides a feature which makes a computational prediction possible
Figure 1
Workflow of the computational approach followed to identify miRNAs
Workflow of the computational approach followed to identify miRNAs. Overall procedure for miRNA, target and homologous regions identification.
Results and discussion
Einverted program from EMBOSS package
Figure 2
EST alignment of selected sequences of
EST alignment of selected sequences of
Additional file 1
predicted pre-miRNAs folding in Compositae. The table contain the information about all pre-miRNAs present in
Click here for file
Figure 3
Similarity of miRNA secondary structures
Similarity of miRNA secondary structures. Stem-loop of structures of miR156 in Cardueae, acc. GE609552, EL372706, EL373856, EL385094 with mfe -66, -59.8, -58.8, 57.4 kcal/mol respectively (a-d); stem-loop structures of miR156 from Arabidopsis and arachis, (Ath-miR156f and Ahy-miR156c) with mfe -64.12 to -56.6 kcal/mol (e-f).
Figure 4
Location and secondary structures of Ath-miR167d and its homologous in artichoke
Location and secondary structures of Ath-miR167d and its homologous in artichoke. Secondary structure of the Ath-miR167d primary transcript (mfe -129.70 kcal/mol) (a). Predicted secondary structure of
BLASTx analysis showed at least three miRNAs within coding regions, (E-values range: 1e-26 to 8e-127), corresponding to miR168a and miR834 in
Experimental identification of mature miRNAs
An experimental assay was developed for
Table 1
List of primer sequences for RT-PCR analysis for cloning and sequencing microRNAs from
Related miRNA
Primer Name
Primer Sequence
Poly(T) adapter
gcgagcacagaattaatacgactcactatagg(t)12vn §
Poly(T) adapter reverse*
§ degenerated bases are indicated by the following one-letter code: C+T = Y; A+C+G = V; A+C+G+T = N.
* Common reverse primers for all miRNAs
Identification of homologous regions between
However, this study revealed that some sequences considered specific only for
Target prediction
RNAhybrid results showed that 7145 and 8730 ESTs in
Additional file 2
RNAhybrid results in
Click here for file
Additional file 3
RNAhybrid results in
Click here for file
Additional file 4
Minimum free energy calculation for each miRNA target, by RNAhybrid. In the first and the second columns are reported the mirBase identifier and accessions numbers. In these two columns, the miRBase ID/accession have been listed by comma, for the species with identical mature miRNA sequence. For each mature miRNA the mfe and mfecut-off, used in the analysis, is indicated.
Click here for file
Blast results and COS identification
The comparative approach herein followed allowed the identification of conserved homologous/orthologous sequences (COS regions) in EST datasets of
Additional file 5
Identified COS regions in
Click here for file
About 8000
Conserved target in COS region
Analyses carried out in COS regions resulted in the identification of miRNA targets, conserved in
Additional file 6
Significant identified miRNA targets. The table contains the significant targets (specificity > 0.8) identified by this pipeline. For each target has been indicated the occurrence find in the ESTs, the average of the occurrence obtained considering two shuffled sequence datasets, the signal to noise ratio and the specificity.
Click here for file
The complete EST sequences analyses, including target and relative functions, are reported in additional file
Additional file 7
Significant target functions. In the table are showed the statistical significant targets and the putative function obtained by comparison of the
Click here for file
In this study, potential targets for miR390 were found within 12 ESTs of
Within the
The confirmed role played by miRNAs in both physiological and pathological processes make them an interesting object of study
The identification of 17 ESTs codifying for miRNA precursors in Asteraceae family, showed that the rate of precursors retrieve is estimated at 0.021%, alike to the real rate found in Arabidopsis (see material and methods), highlighting the validity of the developed method; moreover catm0011, a novel miRNA predicted from sufflower
Identification of putative conserved miRNAs and their precursors
A computational approach to identify potential miRNA precursors was carried out in the EST db of
Additional file 8
Arabidopsis Test 1. The table depicts 221 EST sequences as real pre-miRNA retrieved after BLAST analyses in Arabidopsis EST dataset. The analyses were conducted considering identity > 90%, and pre-micro sequence alignment coverage ≥ 70%, in order to limit the false positive originated from the possible targets.
Click here for file
Additional file 9
Arabidopsis Test 2. In the table are listed the pre-miRNA obtained on the same Arabidopsis EST dataset used for the real pre-miRNA identification (see Table S8), by the bioinformatic approach described in the manuscript. We recognised 196 out of 221, corresponding to 88% of the real precursor present in the data.
Click here for file
Identification of conserved homologous/orthologous EST regions
The BLAST program was used to identify homologous regions within the ESTs of the species under study. In the BLASTn (e-value e10-4) analysis we considered
Target prediction analysis
The RNAhybrid program was used to identify possible binding sites present in the ESTs, following specific base pairing rules. RNAhybrid finds the mfe hybridisation of two RNAs fragments with different lengths, i.e. long (ESTs) and short (mature miRNA sequences), respectively
Statistical target evaluation on COS regions
In order to evaluate the confidence level of the target prediction on the COS regions, for each target the number of occurrences found was confronted with those expected in random samples. To assess the signal-to-noise ratio and specificity level, two approaches were employed: in the first one, for each mature miRNA sequence used in the analysis, 10 randomized sequences were produced, with the same di-nucleotide composition of the source, by means of shuffleseq program
And the signal to noise ratio (SNR) as:
The specificity as function of the signal-to-noise ratio is:
Experimental assays
Plant growth
Seedlings of globe artichoke (
RNA extraction
Total RNA was extracted from leaf (cotyledon and true leaves) of 3-weeks old globe artichoke plants, using Trizol reagent (Invitrogen, UK) followed by RNase-free DNase digestion (RNase-free DNase set and RNeasy Mini Kit, Qiagen GmbH, Germany). Samples concentration was determined by a spectrophotometer (NanoDrop©, Thermo Scientific, USA). Polyadenylation was performed as follows: 2 μg of total RNA were added to 25 μl of a reaction mixture in presence of 2.5 mM MnCl2, 80 μM ATP, 1× reaction buffer and 1 U of
Amplification of miRNAs was carried out from 1 μl of a 1:10 dilution of the reverse transcribed reaction with a common reverse primer, Poly(T) adapter reverse, homologous to 23 nt in the Poly(T) adapter sequence, while the forward primers were chosen according to homologous sequences from miRNAs selected through the
List of abbreviations used
miRNA: microRNA; RISC: RNA-induced silencing complex; AGO: ARGONAUTE; dsRBP: dsRNA binding protein; ESTs: Expressed sequenced tags; COS: Conserved homologous/orthologous sequences; mfe: minimum free energy; NGS: next generation sequencing; PPR: pentatricopeptide repeat; Cu, Zn-SOD: copper/zinc superoxide dismutase.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
DC and MMFS conceived and designed the study. DC carried out the bioinformatic analyses, performed the statistics and helped to draft the manuscript. MMFS performed the experimental assays and drafted the manuscript. DP and GS participated in the design of the study, the discussion of results and helped to draft the manuscript. All authors read and approved the final manuscript.
This work was partially supported by a dedicated grant from the Italian Ministry of Economy and Finance to the National Research Council. Project: "Innovazione e Sviluppo del Mezzogiorno - Conoscenze Integrate per Sostenibilità ed Innovazione del Made in Italy Agroalimentare - Legge n. 191/2009. The authors thank F. Cillo for kindly commenting the manuscript and the anonymous referees for their thorough review and highly valuable comments and suggestions, which significantly contributed to improving the quality of the paper.
This article has been published as part of
A chloroplast DNA inversion marks an ancient evolutionary split in the sunflower family [Asteraceae]
A review of the phylogeny and classification of the Asteraceae
Protective effects of purified safflower extract on myocardial ischemia in vivo and in vitro
Inhibition of cholesterol biosynthesis in primary cultured rat hepatocytes by artichoke [Cynara scolymus L.] extracts
Molecular properties and prebiotic effect of inulin obtained from artichoke (Cynara scolymus L.)
Role of microRNAs in plant and animal development
MicroRNAs and their regulatory roles in animals and plants
High-throughput in vivo analysis of gene expression in Caenorhabditis elegans
Genome-wide gene expression profiling in Arabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant
Novel hydroxycinnamoyl-coenzyme A quinate transferase genes from artichoke are involved in the synthesis of chlorogenic acid
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
MicroRNAs in plants
The expansion of the metazoan microRNA repertoire
Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate
MicroRNAs: genomics, biogenesis, mechanism, and function
MicroRNAS and their regulatory roles in plants
A biochemical framework for RNA silencing in plants
Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions
Nuclear processing and export of microRNAs in Arabidopsis
The functions of microRNAs in plants
Radial patterning of Arabidopsis shoots by class III HD-ZIP and KANADI genes
Control of leaf morphogenesis by microRNAs
Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes
The early extra petals1 mutant uncovers a role for microRNA miR164c in regulating petal number in Arabidopsis
Principles of microRNA-target recognition
Fast and effective prediction of microRNA/target duplexes
RNAhybrid: microRNA target prediction easy, fast and flexible
EMBOSS: The European Molecular Biology Open Software Suite
miRBase: microRNA sequences, targets and gene nomenclature
Vienna RNA secondary structure server
Basic local alignment search tool
Investigation of the microRNAs in safflower seed, leaf, and petal by high-throughput sequencing
Genome-wide analysis of BURP domain-containing genes in Maize and Sorghum
A subclass of myosin XI is associated with mitochondria, plastids, and the molecular chaperone subunit TCP-1alpha in maize
Conservation and divergence of plant microRNA genes
MicroRNAs: at the root of plant development?
Comprehensive prediction of novel microRNA targets in Arabidopsis thaliana
Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis
The Arabidopsis cyclophilin gene family
AtCyp59 is a multidomain cyclophilin from Arabidopsis thaliana that interacts with SR proteins and the C-terminal domain of the RNA polymerase II
Mutant identification and characterization of the laccase gene family in Arabidopsis
Regulation of copper homeostasis by micro-RNA in Arabidopsis
MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis
Essential transition metal homeostasis in plants
Auxin regulation of the microRNA390-dependent transacting small interfering RNA pathway in Arabidopsis lateral root development
Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis
On the expansion of the pentatricopeptide repeat gene family in plants
Chloroplast RNA-binding and pentatricopeptide repeat proteins
Facile means for quantifying microRNA expression by real-time PCR
Differential effects of mild and severe Cucumber mosaic virus strains in the perturbation of MicroRNA-regulated gene expression in tomato map to the 3' sequence of RNA 2


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The hotel was established in 1932 and regarded as the first and most luxurious entertainment place in Shanghai, highly recommend to the leisure travellers,especially who likes shopping. CZ Feb/11
Located in a historic landmark shopping street, the hotel is strategically ideal for business, shopping, sightseeing and leisure in Shanghai, providing easy access to the Bund, Yu Garden, People Square, Shanghai Museum, Grand Theater and Huai Hai Road.
It is a tall building in Euro design.
Lobby is in small size but elegantly decorated.


text:
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Biologics: A Class of Medication Produced by Recombinant DNA TechnologyBY: Muniba Safdar | Category: Biology | Submitted: 2011-02-17 19:27:05
Article Summary: "What is biologics? Information about its composition and Recombinant DNA biologics. Biologics generally refers to biology or to life and living things. It is a class of medication. It includes an extensive range of medicinal products in medicine created by biological processes. Biologics are sequestered from a multiplicity of n.."
Biologics generally refers to biology or to life and living things. It is a class of medication (is something that treats, prevents or alleviates the symptoms of disease or the act of treating with medicines or remedies). It includes an extensive range of medicinal products in medicine created by biological processes. The medicinal products includes blood, vaccine, allergenic (is related to or having the effect of an allergen) gene therapy (is the insertion of normal or genetically altered genes into cells usually to replace defective genes especially in the treatment of genetic disorders), blood components, somatic cells, recombinant therapeutic proteins and tissues.
Biologics can be framed as;
• Proteins
• Sugars
• Nucleic acids
• Living entities (such as tissues and cells)
• Complex combination of these above substances
Isolation of biologics:
Biologics are sequestered from a multiplicity of natural sources such as human, animal, or microorganism. It may be created by biotechnology methods and other medicinal technologies. Biologics that are cellular and gene-based are the most essential forefront of biomedical (is related to the activities and applications of science to clinical medicine) research. There are no other cures available for the treatment of various medical disorders and in order to overcome these situations cellular and gene-based biologics can be used to handle a variety of medical conditions.
Recombinant DNA technology by means of Biologics:
As it is mentioned in the above context, the word "biologics" can be used to state to a comprehensive range of biological products in the field of medicine. However, in a restrictive manner the word biologics is used for a class of medication produced by recombinant DNA technology by means of biological processes. These medications are commonly of three types:
1. There are some substances or materials that are almost same to the body's own key signaling proteins. For example, blood-production stimulating protein erythropoietin (is a glycoprotein secreted by the kidneys that stimulates the production of red blood cells), or biosynthetic (is the production of a chemical compound by a living organism) human insulin and its analogues, or growth hormone.
2. Monoclonal antibodies (any of a class of antibodies produced in the laboratory by a single clone of cells or a cell line and consisting of identical antibody molecules) are similar to the antibodies. These antibodies are used by human immune system to fight with viruses and bacteria. But the monoclonal antibodies are "custom-designed" which means that they are designed through hybridoma (is a hybrid cell resulting from the fusion of a lymphocyte and a tumour cell; used to culture a specific monoclonal antibody) or any other methods. These monoclonal antibodies are just made to target any specific cell type or to stabilize or block any given substance in the body.
For example, Remicade a monoclonal antibody used to cure psoriatic arthritis (is a form of rheumatoid arthritis usually affecting fingers and toes and associated with psoriasis), psoriasis (is a chronic skin disease characterized by dry red patches covered with scales; occurs especially on the scalp and ears and genitalia and the skin over bony prominences), Crohn's disease, rheumatoid arthritis (is a chronic autoimmune disease with inflammation of the joints and marked deformities; something (possibly a virus) triggers an attack on the synovium by the immune system, which releases cytokines that stimulate an inflammatory reaction that can lead to the destruction of all components of the joint) and breast cancer.
3. Receptor constructs or fusion proteins that are generally based on a naturally-occurring receptor interconnected to the immunoglobulin (is a class of proteins produced in lymph tissue in vertebrates and that function as antibodies in the immune response) frame. In terms of pharmacology (is the science or study of drugs: their preparation and properties and uses and effects), immunoglobulin-structure instructs stability whereas receptor are responsible to provide the construct with complete specificity.
For example, immunoglobin CTLA-4 fusion protein technology is used to cure rheumatoid arthritis and to cure chronic plaque psoriasis; immunoglobin G1 fusion protein technology is used.
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text: The BLAUMAR APARTAMENTOS are the ideal destination to spend a fun family holiday. The establishment has apartments of 2 bedrooms. Back at the hotel, a swim in the pool will smooth away the tiredness from a long day full of excitement. Guests can take part in any of the nautical activities arranged by the hotel. Guided tours arranged.