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Rembert's best game of the season came against North Carolina, where he scored 18 points in a 23-point loss. The issue here, however, is that he has not had too many other good games. Rembert has more turnovers than assists, shoots 37 percent from the field, and is unlikely to have a major impact here. He is not an awful defender, but Lyons certainly has the advantage and will likely try to force Rembert into turning the ball over, as he has 16 turnovers in his last three games.
Advantage: Arizona
SG: Nick Johnson (6-3, 200, So.) vs. Jarvis Jones (6-2, 190, Sr.)
Jones has only played in two games this season, but he has made his presence felt, although that may not be a good thing. Jones has 36 total shots this season and has only made 11 of them, including 2-15 from behind the arc. Basically, Jones is a volume shooter that really is not very good at what he does. In addition, Jones already has nine turnovers, though he did have five steals against Ole Miss. Johnson is one of the best perimeter defenders in the country, so if Jones is going to get back on track, it is hard to believe he will do it in this game. Jones is likely going to get his shots up, but they won't be good ones and Johnson will easily have a more efficient game.
Advantage: Arizona
SF: Solomon Hill (6-7, 220, Sr.) vs. Lester Wilson (6-4, 205, Fr.)
Wilson is another volume shooter, but easily the most effective player on the team. He averages around 18 points and 7 rebounds per game, including a 20 and 9 effort against Virginia Tech, 26 and 8 against Charleston Southern, and 14 and 7 against Ole Miss. If Wilson has a bad game, ETSU has no chance of competing, let alone winning. Wilson has the ability to be extremely effective, especially if he gets going from behind the arc, as he is shooting around 40 percent on 74 attempts. The issue for Wilson will be that he just can't guard Hill and Hill may also be playing the best defense of his career right now. Wilson may get his, but Hill will be able to match him throughout the game.
Advantage: Arizona
PF: Brandon Ashley (6-8, 235, Fr.) vs. Hunter Harris (6-7, 220, Jr.)
Hunter is actually a solid player averaging 8 and 7 per game. However, that is basically his ceiling and he is not going to have a major impact beyond that. Ashley has a size advantage here and definitely has higher potential as well. If Ashley gets going early, this is a difficult matchup for Hunter, who has struggled with athletic teams. Harris can be forced into turnovers with a physical defender and although Ashley has not been great on defense this season, he has been good enough. If Ashley plays like he did against Oral Roberts, this matchup is a no-brainer.
Advantage: Arizona
C: Kaleb Tarczewski (7-0, 255, Fr.) vs. Kinard Gadsen-Gilliard (6-5, 250, Jr.)
This is an awful matchup for ETSU and the guess here is that it goes small in order to try and get Tarczewski out of the game. Gadsen-Gilliard has the weight of a big man, but he does not have the height and has struggled in nearly every game this season. His high in points is 12 against Charleston Southern, but he has gone scoreless in three games and scored two points in two other contests. Gadsen-Gilliard's season-high in rebounds is five and he has played more than 25 minutes in a game only twice. Basically, Tarc should be able to put together a strong game, but it will be interesting to see what the opponent does to try and take him out of it.
Advantage: Arizona
Bench: Mario Stramaglia will be the first guard off the bench and has gotten the minutes of a starter at times, as he went for 32 minutes against Ole Miss and 27 against UNC. The issue is that he had a combined 11 turnovers in those games and does not offer much in terms of being a primary ball-handler. John Walton will come off the bench as well, but the forward has not done a lot this season, despite averaging about 20 minutes per game. We're not sure Arizona is going to play a game this season where it does not have a bench advantage and it certainly will not be this one.
Advantage: Arizona
Coaching: Sean Miller vs. Murry Bartow
Bartow has the best winning percentage of any coach in school history, but we don't need to analyze this matchup too much. Miller has his team off to a hot start and Bartow does not. In addition, they are obviously coaching at different levels. This game probably will not come down to coaching but, if it does, Arizona has the advantage.
Advantage: Arizona
Prediction: There is absolutely no reason why this game should be close. ETSU is not a good shooting or rebounding team and simply does not have the size to go toe-to-toe with Arizona. The Wildcats will try to get an early lead and get some other guys minutes and should be able to do so. We don't see this game being close for long due in large part to the fact that East Tennessee State just can't keep up with Arizona offensively.
Arizona 83, East Tennessee State 60


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The Sokoto fountain above ground basin stands this ribbed monolithic sculpture at 37 inches tall and is sure to add beauty and focus to your garden decor. The water flows seamlessly down the detailed sides. A bad of River Stones are included for added enhancement.View More Items in This Collection
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Hillview Chairs Collection
In honor of the Amish tradition of superior craftsmanship with a modern-day appreciation for variety, the Hillview designers have introduced the fantail back slat design in hopes of pleasing those who have a penchant for something unique.
Set to impress, this fantail desk chair is both comfortable and aesthetically appealing -- an excellent choice for your office. Choose from white quarter-sawn oak, cherry or traditional oak; side and arm-chair designs.
As with all of our office furniture at DutchCrafters, this Fantail Desk Chair is handcrafted in the USA. It is built with the highest commitment to quality in wood selection, workmanship, hardware and finishing.
Rated A+ by the Better Business Bureau, DutchCrafters is a name you can trust. Give us a call at 866-272-6773 and let us help you with your selection of office furniture. You'll see why thousands of customers have selected DutchCrafters for their superior quality, solid wood office furniture.
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Visitor Information
GuidelinesVisitor guidelines are developed on a case-by-case basis as determined by a patient's care needs. It is recommended that visitors call ahead before they come to visit as the patient's schedule changes daily.
- Rehabilitation unit quiet time begins at 10 p.m.
- Please refrain from using your cell phone during therapy sessions, while nursing is passing medications or when the doctor is making rounds with your family member.
- Close family and friends who are involved in the patient's care are welcome to attend therapies to increase their understanding of patient's care needs.
- Visitors may need to visit on evenings and weekends depending on the rehabilitation patients' endurance.
- Please use the Chestnut Street entrance. The rehabilitation unit is located on the third floor on the north end of the building.
- Guest trays for family or friends can be ordered so that you can share a meal with the patient. Please discuss this with your patient's nurse. This needs to be arranged in advance and payment arranged.
- The South Campus deli is located on the ground floor and is open Monday through Friday from 8:00 a.m. to 1:30 p.m.
- Children ages 12 and under may visit if directly supervised at all times.
- Staff may make recommendations regarding number of visitors and length of visits as patients often need rest between treatments to maximize their improvement.
- Patients have the options of specifying who can visit and when.
- PeaceHealth St. Joseph is a non-smoking facility.
Recreation RoomThe recreation room is a wonderful place for friends and family members to visit patients. There are a variety of activities available for free time use, including a television, DVD/VCR, music, keyboard piano, games, puzzles, art supplies, books, magazines and the daily newspaper, the Bellingham Herald.
The recreation room computer is available on a first come, first serve basis. Our unit is set-up with access to wireless Internet if you wish to bring in your personal laptop.
Grabow Therapy & Wellness Center
3217 Squalicum PkwyBellingham, WA 98225


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Gilfix and La Poll In the News and Media
At Gilfix & La Poll Associates, our attorneys are active participants in the field of elder law and estate planning, and have been called on by the press to discuss developments and activities related to the law. In addition, Michael Gilfix often appears as a guest on San Francisco's KGO Newstalk 810 AM Radio. We have collected some news articles, podcasts and videos here. Please note that they do not constitute legal advice. Contact us to answer any questions.
In the News
Mesh Multigenerational Planning with New Economy Ideas
By Michael and Mark Gilfix
Broaden client service by giving estate planning a multigenerational scope and being alert to new economy opportunities. This would represent a paradigm shift in methods to serve client needs.
A New Paradigm: Truly Multigenerational Planning
Help Families Achieve a Diversity of Shared Goals.
Regardless of wealth, most members of our client communities would benefit from multigenerational planning.
An Estate Plan Built For Special Needs
By RACHEL EMMA SILVERMAN, wsj.com -
Gabe Molitor is no ordinary trust-fund kid: He has epilepsy and Asperger's syndrome, a form of autism. His mother, Shelby Valentine, recently set up what's known as a special-needs trust, which will provide funding to pay for some of her 30-year-old son's expenses when Ms. Valentine and her husband are no longer able to care for him...
To Protect an Aging Parent's Assets, Plan Ahead
By Mike Krantz, Usa Today. This is a question and answer piece. Q: My father may be put into a nursing home and would like to protect his investments, including his home and $250,000 in cash and stocks. Is there a way to do this? A: ...
June 1: Len Tillem Show
Avoiding gift and estate taxes - steps you can take now.
September 2: Len Tillem Show
Medi-Cal and planning for long-term care.
Mike Gilfix talks about what one should expect when coming in to see the attorneys at the firm. At Gilfix & La Poll, you can take care of all of your Estate Planning needs.


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|Stephen Colebourne <scolebou...@btopenworld.com>
|Re: [PROPOSAL][all] Break dependency on commons-build
|Sat, 11 Mar 2006 22:49:55 GMT
Dennis Lundberg wrote: > Stephen Colebourne wrote: > It looks good. Personally I like this l&f better than the "old" one, but > that's a matter of taste I guess. I deliberately chose to 'enhance' a few things :-) > - The breadcrumbs line uses a smaller font with less padding, in your > example > - Copyright notice is now smaller and to the right instead of left > - Navbar headings are now underlined > - Navbar has arrows indicating folded menus, for "Project Info" and > "Project Reports" > - Icons for external links have been added > - The content area seem identical to me. Deliberate > - Links in the non-content areas have a slightly different color Ah. Maybe should make a little brighter, although the old colour was too bright IMHO. > - The sections "About us" and "Commons Resources (Unofficial)" are > missing from the navbar. I guess this is due to not using the menus from > commons-build. This is a problem, and I can't figure out why. You may be able to help here. No matter what entity I use, the sections aren't picked up. All I can think is that its caused by no longer using the commons-site.jsl. Stephen --------------------------------------------------------------------- To unsubscribe, e-mail: email@example.com For additional commands, e-mail: email@example.com
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Rated as the 20th-best defensive tackle in the nation after a standout career at Bay High School in Panama City, Fla., he was set to attend Florida State but didn't qualify for academic reasons. He turned his attention to the University of Southern Florida, but was denied entry despite meeting NCAA qualifying requirements.
"It was frustrating," Thornton said. "I learned about college football, it's a business more than anything. I look back and I don't have any regrets. Looking back on it, it was a blessing."
Undeterred, Thornton ended up at Southern Miss and embarked on a standout career. He was voted team captain as a senior and closed his career with 116 tackles (58 solo), with 37 for losses including 5.5 sacks, on his way to second-team all-Conference USA honors. While his tenure included a 12-win sophomore season, it was followed by a crushing 22-game losing streak that somehow didn't crush his spirit.
"You push through and fight every day," he said. "I felt like, I had an opportunity to play college football. A lot of times when I used to go home, people reminded me of that. They reminded me others would like to be in my position. Every game, every day … was a blessing."
That includes the game that finally snapped the Golden Eagles' dubious streak. Of course, Thornton was on the sideline nursing a deep bone bruise in his right leg suffered the week before.
"It was hard, but I was happy because we didn't lose all our games," he said. "We were trying to bring back the tradition of winning. I was glad to be a part of that win. I was disappointed I couldn't play in it and be a part of it, but it was still my team and I feel like I was still a leader. After the game, all of my teammates came over and gave me a hug and said they got the win for me."
Now, the 6-foot-3, 304-pounder will have the chance to contribute to some wins at the pro level. He was a either a "fast riser" — the NFL's head scout, Dave-Te' Thomas, had Thornton as his "most underrated" nose tackle — or a projected fifth- or sixth-round pick, depending on who you believe. But the former seemed to be where Packers general manager Ted Thompson was leaning when he selected Thornton with the 85th overall pick in the third round.
"To be honest with you, I didn't really have high expectations of going high in the draft," Thornton said. "I didn't know a lot of teams were willing to take me. I told my agent, 'Don't tell me anything. Keep it to yourself.'
"I had no idea (Green Bay) was interested."
But they were. Very interested, as a matter of fact. Just two picks after Notre Dame defensive tackle Louis Nix III went off the board – nearly two rounds later than many thought – Green Bay chose the unheralded and largely unheard of Thornton over higher-ranked prospects like Penn State's DaQuan Jones, Louisiana Tech's Justin Ellis and Tennessee's Daniel McCullers.
"Khryi can catch your eye," Thompson said when asked what separated Thornton from the remaining players. "He played on a team that didn't win that many games but he's extraordinarily quick off the ball and has the ability to penetrate and get in the backfield and pursue laterally and that sort of thing. So, we think he gives us a chance to have a little juice."
"Juice" in scout speak equates to energy, hustle and, hopefully, some pass rush. He lined up all along the defensive front at Southern Miss and could play either the three-technique – shading the outside shoulder of the guard as more of an inside rusher -- or five-technique end – straight across from the offensive tackle with responsibility for two gaps. He'll be battling for snaps on a mostly young defensive line stocked with players like Mike Daniels, Josh Boyd, Jerel Worthy and Datone Jones, not to mention veterans like B.J. Raji, Letroy Guion and Julius Peppers.
But Packers defensive line coach Mike Trgovac saw enough tape of Thornton's tangibles and demeanor to be excited about adding him to the mix.
"I think the biggest thing when you watch a guy from a 1-24 team, or whatever their record was, is his competitiveness throughout those games," Trgovac said. "Sometimes it's hard when you're losing all the time to keep your spirits going and he didn't have a problem with that.
"The best thing about him is probably his get-off off the ball. He's got really good, quick feet. The guy is, what, 305 pounds and ran a five flat (4.99) 40. That's the thing that impressed me the most, was some of the movement defenses that he did he was able to get into the gap fast and shoot that gap."
Between the NFL Combine and the NFLPA Collegiate Bowl, the versatile Thornton caught the eyes of scouts, whether he realized it or not. And as a member of the Packers, the chances of him seeing 22 losses over the length of his contract seem slim. Suddenly, the advice he gave to his teammates during those tough times is coming to pass.
"I told guys the eye in the sky never lies. If you're down 40 or up 40, play hard to the whistle and, hopefully, somebody will see you."
And they did.
Agree or disagree?: Discuss hot Packers topics in our, free forums. Leave publisher Bill Huber a question in the subscribers-only Packers Pro Club forum.
W. Keith Roerdink has covered the Packers since 1992. E-mail him at email@example.com.


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Fri December 9, 2016 - 7:30 PM
Denver, CO, 2016
Saint John's Cathedral
1350 Washington Street
Denver, CO
It is Kantorei's 20th Anniversary. Kantorei celebrates by performing your favorite Christmas music by request! Send your requests for the music you want to hear by visiting www.kantorei.org, or by emailing at firstname.lastname@example.com...See More


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Targeting the motor regulator Klar to lipid droplets
Here we describe a new mutant allele of
Our analysis demonstrates that droplet targeting of Klar occurs via a cis-acting sequence and generates a new tool for monitoring lipid droplets in living tissues of
Lipid droplets are the intracellular sites for storage of neutral lipids. Long dismissed as inert inclusions, they are now recognized as dynamic organelles with a myriad of functions well beyond fat storage [reviewed in,
Despite the ubiquity and potential biological significance of droplet motion, its mechanism is not well understood
At least in part, specificity of droplet motion is achieved via distinct motor regulators that are present exclusively on lipid droplets. For example, in both mammals and flies, members of the Perilipin family modulate droplet motion
Klar's role is not limited to lipid droplets; in different cells, Klar controls distinct transport processes. In early embryos, it regulates the bidirectional motion of lipid droplets
This tissue-specific targeting of Klar correlates with isoform variation
For Klar α, there is ample support for this model. Its C-terminal region contains a 60 aa KASH (Klarsicht/ANC-1/Syne Homology) domain. KASH domains typically localize to the outer nuclear envelope, and together with SUN domain proteins in the inner nuclear envelope establish a bridge linking cytoplasmic and nucleoplasmic proteins
Available evidence is consistent with the notion that the unique C-terminal region of Klar β has an analogous role in targeting to lipid droplets. For example, in cultured cells, this so-called LD domain is sufficient to recruit RFP to lipid droplets
To examine the role of specifically the LD domain, we identified a new
Identifying a
The transcripts for Klar α and β diverge after exon 15, followed either by exons 16, 17 and 18 (Klar α) or exon 15ext (Klar β)
To address this issue, we searched for new
Many lines from the mutant collection are mixtures of homozygotes and heterozygotes; the latter carry not only the mutagenized chromosome but also a balancer chromosome. In particular, for strain Z3-3772, the TILLING analysis had employed DNA from heterozygotes as in the extant stock homozygotes are rare to non-existent. When we extracted the non-balancer chromosome from this stock and sequenced exon 15ext, the predicted protein sequence of the corresponding LD domain was identical to the canonical sequence of Klar (Figure
Figure 1
Sequence of the LD domain in various strains and species
Sequence of the LD domain in various strains and species. (A) Comparison of
To reconcile this observation with the TILLING results, we hypothesized that the mutation in the parental line might exist on the balancer chromosome, TM6B. We generated flies heterozygous for the TM6B chromosome from line Z3-3772 and for
This deletion is not a general feature of the TM6B balancer chromosome. Although many of the lines from the mutant collection were analyzed as heterozygotes, the deletion was recovered only once. In particular, our screen identified sequence variations for 19 other lines for which DNA from heterozygous flies had been characterized; but for none of these lines was the 25 bp deletion identified. We also extracted a TM6B chromosome from a different stock of the collection and found that the 25 bp deletion was not present. Thus, this deletion in exon 15ext did not pre-exist on the TM6B chromosome and apparently arose spontaneously since the mutagenesis scheme employed to generate the collection
What is the consequence of this change in the genomic sequence? The 25 bp deletion in exon 15ext shifts the open reading frame and should result in a truncated LD domain: While the initial 50 amino acids are unchanged, the C-terminal 64 residues are replaced by an unrelated five-amino-acid sequence (Figure
To determine if
Table 1
Predicted sequence variation in KlarMW
Observed change
Exon affected
Ser to Thr
a, b
Ser to Thr
a, b
Ser to Ala
a, c
Asp to Glu
a, b, c
Ser to His
a, b, c
Thr to Ser
a, b, c
Ile to Asn
a, b, c
Frame shift
See Fig. 1A
Thr to Ser
a, b
Ser to Ala
a, b
The protein coding exons of
a present in KlarZ
b present in a wild-type Klar α cDNA (GenBank
c present in various wild-type
The LD domain is necessary for droplet localization
The identification of
We first asked if the mutant KlarMW protein was expressed. We extracted proteins from early embryos of various genotypes and detected Klar by Western analysis (Figure
Figure 2
KlarMW is expressed in embryos, but not localized to lipid droplets
KlarMW is expressed in embryos, but not localized to lipid droplets. (A) Klar Western of embryos (cycle 14/Phase II) of various genotypes. Since
To test if the mutant Klar was present on lipid droplets, we employed the assay previously used
The LD domain truncation disrupts lipid-droplet transport in embryos
During early embryogenesis, lipid droplets display stereotyped shifts in their overall distribution as the relative balance of plus- and minus-end motion changes in a temporally controlled manner
Figure 3
Klar β is important for the correct balance between plus- and minus-end motion. In the absence of Klar β, the global droplet distribution in Phases I and II is similar to the wild type, but becomes dramatically different in Phase III: lipid droplets remain around the center, instead of shifting back to the periphery. To assess if the LD domain contributes to Klar's function in this transport process, we compared
Sequence variation in the LD domain
The TILLING approach had uncovered a second strain (Z3-1770) with mutations in exon 15ext. We verified those changes by genomic sequencing; they are predicted to result in amino-acid changes at five positions (Figure
To identify other positions in the LD domain at which variation can be tolerated, we compared the sequence of this domain across seventeen fly species (Figure
The LD domain truncation supports nuclear migration in photoreceptors
Besides droplet transport, the best-characterized role of Klar is in the migration of nuclei in developing photoreceptors
To reveal the position of photoreceptor nuclei in third-instar eye discs, we employed the marker Elav (Figure
Figure 4
A GFP-tagged LD domain localizes to embryonic lipid droplets
The previous analysis established that the LD domain is necessary
Figure 5
GFP-LD distribution in early embryos
GFP-LD distribution in early embryos. (A, B) Western analysis of wild-type (WT) and GFP-LD expressing (GL) animals. GL samples are from females carrying both a GFP-LD transgene and the matα4-Gal4-VP16 driver or from embryos laid by these females. GFP-LD was detected with anti-GFP antibodies; corresponding WT samples demonstrate specificity of detection. (A) GFP-LD is present in both ovaries and embryos. (B) GFP-LD is expressed at similar levels in Phase I, II, and III embryos. Tubulin serves as loading control. (C, D) GFP-LD expressing embryos examined live by confocal microscopy. In otherwise wild-type embryos, GFP-LD is present in distinct puncta that accumulate around the central yolk (C). In embryos mutant for Halo, GFP-LD puncta accumulate just under the nuclei (D); these puncta are shifted outwards relative to the embryos in C. Top: whole-embryo view. Bottom: detail of the embryonic periphery. The embryos in C and D are age-matched (top: early Phase II; bottom: mid Phase II). Scale bars in D represent 60 μm (top) and 10 μm (bottom), respectively. (E) GFP-LD expressing embryos imaged live by epifluorescence microscopy. Comparison to a wild-type embryo demonstrates that most of the signal is due to GFP-LD. Global distribution of GFP-LD in Phases I, II, and III mimics the distribution of lipid droplets at these embryonic stages (Fig. 3A); in particular, GFP-LD is spread throughout the periphery in Phase III if endogenous Klar β is present, but not if it is absent. (F) Even when expressed in the absence of endogenous Klar β, GFP-LD is present in discrete puncta (scale bar = 6 μm). (G) Triglyceride levels in wild-type and GFP-LD expressing embryos (0-1.5 hrs old) are very similar. Error bars represent the standard deviation from two different experiments.
To determine the intracellular distribution of GFP-LD, we detected GFP fluorescence in living and in fixed embryos or stained fixed embryos with anti-GFP antibodies. All three methods revealed that the fusion protein was present in discrete puncta (Figure
Additional file 1
Motion of GFP-LD puncta in living embryos. Images were acquired by confocal microscopy at 2.5 images per second. Playback at 12 frames per second. Scale bar = 7.5 μm.
Click here for file
Additional file 2
Motion of GFP-LD puncta in living embryos. Images were acquired by confocal microscopy at 1.35 images per second. Playback at 6 frames per second. Scale bar = 7.5 μm.
Click here for file
If GFP-LD indeed marks lipid droplets, its distribution should change predictably if lipid-droplet transport is altered. Inward transport of lipid droplets in Phase II requires the transport regulator Halo; in its absence, lipid droplets accumulate close to the plasma membrane, under the nuclei, rather than around the yolk in the center
Finally, at higher magnification, the GFP-LD structures appeared as rings (Figure
Figure 6
GFP-LD puncta are lipid droplets
GFP-LD puncta are lipid droplets. (A, B) GFP-LD co-purifies with lipid droplets. (A) Pre-cellularization embryos were embedded in agar and centrifuged. In the image, the embryos are arranged such that the lipid-droplet layer points up. GFP-LD is highly enriched in the droplet layer, whether or not the embryos express endogenous Klar β. Under the same imaging conditions, autofluorescence in wild-type embryos is negligible. (B) Lipid droplets were isolated from wild-type (WT) and GFP-LD expressing (GL) embryos by floatation. Equal amounts of protein from embryo lysates (Lys) and from the droplet fraction (DF) were compared by Western analysis. The cytoplasmic protein tubulin is absent from the droplet fraction. Both the lipid-droplet protein LSD-2 and GFP-LD are highly enriched in the droplet fraction. GFP-LD was detected as in Fig. 5. (C, D) GFP-LD expressing embryos were fixed and stained with Nile Red to reveal lipid droplets. GFP-LD is present in rings around lipid droplets; intensity of GFP-LD signal varies between droplets (scale bar = 5 μm). Panel D shows a magnified view of parts of panel C.
There are two unexpected patterns of the GFP-LD signal. First, it is present in fairly uniform rings, rather than the discrete spots observed for endogenous Klar β
GFP-LD localizes to droplets in the absence of endogenous Klar β
The above analysis suggests that the LD domain is sufficient to target an unrelated protein to lipid droplets. This conclusion, however, might be misleading if the LD domain were a dimerization motif. In that case, GFP-LD could physically interact with full-length Klar β and would be targeted to lipid droplets secondarily, even if full length Klar were to bind to the droplets via other domains. Although the analysis of
The GFP-LD fusion co-purifies with lipid droplets
Lipid droplets are rich in neutral lipids and have a low buoyant density. This property makes it possible to biochemically separate droplets from other cellular structures. We therefore asked if GFP-LD copurifies with lipid droplets. In a first test, we employed
The LD domain mediates droplet localization in many cell types
How proteins are targeted to lipid droplets is not well understood
We first examined ovaries since lipid droplets are abundant in nurse cells and in oocytes from mid-oogenesis onwards. GFP-LD expressed with a Gal4 driver specific for the female germ-line (matα4-Gal4-VP16) accumulates during oogenesis (Figure
Figure 7
GFP-LD fusion protein localizes to lipid droplets in many types of cells
GFP-LD fusion protein localizes to lipid droplets in many types of cells. The intracellular distribution of GFP-LD was examined in animals expressing GFP-LD in the female germ line (A, B, C: matα4-Gal4-VP16 driver) or in somatic cells (D, E, F: Act5C-Gal4 driver). Colocalization with Nile Red signal (A, B, D, E, F) or enrichment in the lipid-droplet layer after centrifugation (C) indicates that GFP-LD is associated with lipid droplets. (A) Stage 13 oocyte. (B) Stage 10 nurse cells. (C) Egg chamber after centrifugation. Bright-field microscopy (BF) reveals distinct layering. Prominent brown lipid-droplet layers are evident in the nurse cells (left) and the oocyte (right); the oocyte also shows a gray yolk layer. GFP-LD is enriched in the droplet layers of both nurse cells and oocyte. The merged image also shows yolk autofluoresence in blue. (D) Follicle cells. (E) Larval salivary gland. (F) Wing imaginal disc. In all cases, the intensity of GFP-LD signal varies dramatically between lipid droplets. GFP-LD signal was also present, at lower levels, elsewhere in the cell (
Using the ubiquitously active Act5C-Gal4 driver, we also expressed GFP-LD in somatic cells. In a number of adult and larval tissues, we found discrete GFP puncta, including in follicle cells in the adult ovary, larval salivary glands, and imaginal discs. These structures are lipid droplets, as they appear as rings at higher magnification and co-label with Nile Red (Figure
In summary, targeting of the LD domain to lipid droplets is not an embryo-specific phenomenon. GFP-LD also localizes to lipid droplets in the female germ line and in a number of somatic tissues. In addition, an analogous RFP-LD fusion protein localizes to lipid droplets in cultured
Because the
Droplet targeting by the LD domain
Our analysis of GFP-LD fusions suggests that targeting of Klar β to lipid droplets is a multi-step process. In many cells, GFP-LD strongly accumulates on lipid droplets (Figure
Nevertheless, GFP-LD clearly accumulates strongly on lipid droplets in many tissues, forming characteristic ring structures. Intriguingly, this distribution is distinct from the distribution of full-length Klar β, which is present in discrete dots on embryonic droplets
What is the molecular mechanism by which the LD domain, a region just 114 amino acids in length, is recruited to lipid droplets? Evolutionary conservation and mutational analysis point to a 46 amino acid region as critical for targeting (Figure
Since GFP-LD localizes to the droplet surface, it might potentially interfere with lipid metabolism. For example, in cultured mammalian cells, overexpression of PLIN2 promotes lipid-droplet accumulation, presumably by shielding the droplets from access by lipases
Why is GFP-LD distribution not uniform?
It is striking that not all lipid droplets accumulate GFP-LD to the same level. Lipid droplets in close proximity in the same cell can have dramatically different GFP-LD signal (Figures
Currently, we cannot distinguish whether GFP-LD is preferentially recruited to certain types of droplets existing naturally or whether GFP-LD expression causes differences between droplets. For example, Gal4 drivers often show mosaic expression in nurse cells
The role of the LD domain for droplet transport
The LD domain is necessary not only for droplet localization of Klar β, but also for Klar β's function in regulating droplet transport. Our results indicate that although the LD domain is sufficient for droplet localization, by itself it does not mediate Klar's transport functions. When expressed in the Klar β null background, most GFP-LD signal remains in the yolk sack in Phase III (Figure
Although it is conceivable that GFP-LD expression levels were simply not high enough for rescue of the transport defects, we disfavor this explanation since a full-length, GFP-tagged Klar β construct expressed at much lower levels is sufficient to profoundly alter droplet motion (YVY and MAW, unpublished observations). We therefore conclude that proper regulation of droplet motors requires the N-terminal region of Klar β and not just the LD domain. This model is further supported by the fact that Klar α shares those N-terminal regions and is also involved in motor regulation.
Whether the LD domain simply targets Klar β to lipid droplets or has additional functions is not yet clear. For example, because in yeast the LD domain can interact with the droplet protein LSD-2, it has been suggested that it participates in transmitting developmental signals from LSD-2 via Klar to motors
However, our data suggest that such effects, if they exist at all, do not alter the net outcome of transport. GFP-LD expressing embryos displayed the same transparency changes as the wild type: the embryo periphery became transparent in Phase II and cloudy in Phase III (Figure
Since LD has the ability to bind to lipid droplets in a wide range of cells, Klar β may control droplet motility in many tissues. In
To test the function of the LD domain of Klar β, we generated inducible GFP-LD fusion constructs and identified a
Fly stocks
Oregon R was used as the wild-type stock. The stocks carrying
To generate the GFP-LD expressing flies, GFP was amplified from pEGP-C1 (Clontech) and cloned into the
TILLING, genomic sequencing, and sequence analysis
TILLING analysis was performed by the Fly-TILL service as described
DNA primers were created to bookend individual exons of
To determine the pattern of evolutionary conservation in the LD domain, we first identified the likely
The helical wheel in Figure
Living embryos were centrifuged to separate lipid droplets from other cellular components, as described
To stain lipid droplets, dechorionated embryos or dissected fly tissues were fixed in 4% formaldehyde in PBS for 10-15 min using standard procedures
Two approaches were employed to examine GFP-LD fluorescence in living embryos. For confocal microscopy, embryos were hand-dechorionated, placed in halocarbon oil on a glass slide, and covered with a cover glass that was supported by spacers. For epifluorescence microscopy, embryos were placed into halocarbon oil on a glass slide. The halocarbon oil turns the chorion transparent (see Figure
Micrographs were acquired on a Leica SP5 confocal microscope or a Nikon Eclipse E600 fluorescence microscope with a 4MP Spot Insight camera. Images were processed in Adobe Photoshop and assembled with Adobe Illustrator.
Western analysis
For Western analysis, proteins were typically separated on a 10% SDS PAGE gel and transferred to PVDF membranes using standard Towbin or CAPS transfer. Mouse anti- alpha tubulin (Sigma-Aldrich) was used at 1:10,000, and mouse anti-GFP (Roche) at 1:1000. LSD-2 was detected with a rabbit polyclonal anti-serum
Droplet purification
To generate samples enriched in lipid droplets, we adapted the protocol described previously
Triglyceride measurements
Embryos were collected for 1.5 hr, dechorionated with 50% bleach, and resuspended in triton salt solution
Authors' contributions
YVY generated and characterized the GFP-LD transgenes, contributed to the functional analysis of
We thank the Bloomington
Lipid Droplets Finally Get a Little R-E-S-P-E-C-T
Lipid droplets: a unified view of a dynamic organelle
Fat on the move: intracellular motion of lipid droplets
Translocation and utilization of fungal storage lipid in the arbuscular mycorrhizal symbiosis
Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole
The phosphorylation of serine 492 of perilipin A directs lipid droplet fragmentation and dispersion
Temporal and spatial variations of lipid droplets during adipocyte division and differentiation
PLD1 and ERK2 regulate cytosolic lipid droplet formation
The gregarious lipid droplet
Noninvasive Imaging of Protein Metabolic Labeling in Single Human Cells Using Stable Isotopes and Raman Microscopy
The lipid droplet proteome reveals that droplets are a protein storage depot
A role for lipid droplets in inter-membrane lipid traffic
Hepatitis C Virus Core Protein Induces LIpid Droplet Redistribution in a Microtubule- and Dynein-Dependent Manner
Dynein-mediated cargo transport in vivo: a switch controls travel distance
Coordination of opposite-polarity microtubule motors
Role of dynactin in endocytic traffic: effects of dynamitin overexpression and colocalization with CLIP-170
Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets
A determinant for directionality of organelle transport in Drosophila embryos
Developmental regulation of vesicle transport in Drosophila embryos: forces and kinetics
Regulation of lipid-droplet transport by the Perilipin homologue LSD2
PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores
Organelle-specific Control of Intracellular Transport: Distinctly Targeted Isoforms of the Regulator Klar
Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane
Molecular analysis of the klarsicht gene and its role in nuclear migration within the differentiating cells of the Drosophila eye
Glued participates in distinct microtubule-based activities in Drosophila eye development
Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons
Drosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye
A nuclear-envelope bridge positions nuclei and moves chromosomes
Nuclei take a position: managing nuclear location
Drosophila klaroid encodes a SUN domain protein required for Klarsicht localization to the nuclear envelope and nuclear migration in the eye
The Zuker collection: a resource for the analysis of autosomal gene function in Drosophila melanogaster
Fly-TILL: reverse genetics using a living point mutation resource
TILLING. Traditional mutagenesis meets functional genomics
Retention of induced mutations in a Drosophila reverse-genetic resource
In-vivo centrifugation of Drosophila embryos
The functions of klarsicht and nuclear lamin in developmentally regulated nuclear migrations of photoreceptor cells in the Drosophila eye
PERILIPIN-dependent control of lipid droplet structure and fat storage in Drosophila
A proposed model of fat packaging by exchangeable lipid droplet proteins
Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation
Proteins under new management: lipid droplets deliver
Characterization of the Drosophila lipid droplet subproteome
Drosophila Lipid Storage Droplet 2 gene (Lsd-2) is expressed and controls lipid storage in wing imaginal discs
Drosophila Perilipin/ADRP homologue Lsd2 regulates lipid metabolism
Genomic consequences of background effects on scalloped mutant expressivity in the wing of Drosophila melanogaster
A Drosophila gain-of-function screen for candidate genes involved in steroid-dependent neuroendocrine cell remodeling
Quantitative genomics of starvation stress resistance in Drosophila
A molecular link between FGF and Dpp signaling in branch-specific migration of the Drosophila trachea
Gene expression profiling of the developing Drosophila CNS midline cells
Role of the proline knot motif in oleosin endoplasmic reticulum topology and oil body targeting
Hydrophobic and basic domains target proteins to lipid droplets
ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts
Adipocyte differentiation-related protein reduces the lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover
Rab18 localizes to lipid droplets and induces their close apposition to the endoplasmic reticulum-derived membrane
Gal4 in the Drosophila female germline
In vivo analysis of MT-based vesicle transport by confocal reflection microscopy
Control of Fat Storage by a Drosophila PAT Domain Protein
The reproductive relationships of Drosophila sechellia with D mauritiana, D. simulans, and D. melanogaster from the Afrotropical region
Natural variation of the amino-terminal glutamine-rich domain of Drosophila Argonaute2 is not associated with developmental defects
Looking at embryos
Fluorescent analysis of Drosophila embryos


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This is a typical budget city hotel offering 33 clean accommodation units. It has air conditioning and was renovated in 2011. Guests are welcomed in the lobby which offers a 24-hour reception and a 24-hour check-out service, as well as lift access to upper floors. Internet access is also available at the hotel.
This Kuala Lumpur hotel is located opposite a big shopping centre. It is a 10-minute walk from the hotel to Sunway Pyramid and Sunway Lagoon Theme Park is also within walking distance. A lot of restaurants, bars, cafes and supermarkets are to be found near to the hotel. Public transport is available nearby too. The hotel can be reached within 40 minutes' drive from Kuala Lumpur International Airport and Kuala Lumpur Sultan Abdul Aziz Shah Airport is roughly 30 km away
The rooms come with an attached en suite bathroom with a standing shower and a hairdryer. Guests can look forward to a good night's rest on their double bed. They are fully equipped with individually regulated air conditioning, tea and coffee making facilities, a dressing table, a wardrobe and an LCD TV with satellite channels. Further amenities include a direct dial telephone and Internet access.
The hotel accepts cash payments only.
The check-in time is at 2 pm and check-out is at 12 noon.
Room Facilities:
Direct dial telephone
Internet access
Tea Coffee
Double bed
Individual airconditioned


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KVPY Application Form 2015, KVYP Scholarship 2015 Apply Online, KVPY Application Form & Scholarship 2015
The KVPY Scholarship are depend on the KVPY Applicant form. The Form are available for that students that are eligible or interested in the KVPY Scholarship. The KVPY Scholarship provides those students they really need that scholarship to continue their job.
KVYP Scholarship Details
Kishore Vaigyanik Protsahan Yojana Funded by Dept of Science and Technology, Govt of India and administered by the Indian Institute of Science, Bangalore. Rs. 5,000-7,000/- per month plus 4 months fellowship as Contingency Grant p.a. Applications are invited from School and College Students (Indian Cities) Interested in Pursuing Courses in Basis Science and Careers in Research in Basic Science Fields. The KVPY Fellowships are given only to Indian Nationals to Study in India (OCI and PIO's are not eligible) Students enrolled in an undergraduate course in Basic Sciences, that is, B.Sc./B.S./B. Stat./B. Math./Int. M.Sc./M.S. in Chemistry, Physics, Mathematics, Statistics, Biochemistry, Microbiology, Cell Biology, Ecology, Molecular Biology, Botany, Zoology, Physiology, Biotechnology, Neurosciences, Bioinformatics, Marine Biology, Geology, Human Biology, Genetics, Biomedical Sciences, Applied Physics or Geophysics
Stream SA
Students enrolled in XI Standard (Science Subjects) during the academic year 2013-14 and have secured a minimum of 80% (70% for SC/ST/PWD) marks in aggregate in MATHEMATICS and SCIENCE subjects in the X Standard Board examination
Stream SX
Students enrolled in XII Standard/ (+2) (Science subjects) during the academic year 2013-14 and aspiring to join an undergraduate program in Basic Sciences (B.Sc./B.S./B.Stat./B.Math./Int. M.Sc./M.S.) for the session 2014-15 provided they have secured a minimum of 80% (70% for SC/ST/PWD) marks in aggregate in MATHEMATICS and SCIENCE subjects (Physics/Chemistry/Biology) in the X Standard Board Examination.
Stream SB
Students enrolled in the 1st year of B.Sc./B.S./B.Stat./B.Math./Int. M.Sc./M.S. during the academic year 2013-14 and having secured a minimum of 60% (50% for SC/ST/PWD) marks in aggregate in SCIENCE subjects (Physics/Chemistry/Biology/Mathematics) in the XII Standard / (+2) Board Exam
In the 1st year final examination of B.Sc./B.S./B.Math./B.Stat./Int. M.Sc./M.S. they must secure 60%(50% for SC/ST/PWD) marks before taking up the fellowship.
Their fellowship will be activated when they enroll in an undergraduate course in Basic Sciences (B.Sc./B.S./B. Stat./B. Math./Int. M.Sc./M.S.) after XII Standard (+2) securing a minimum of 60% (50%for SC/ST/PWD) mark or equivalent grade.
Application Form Eligibility Criteria KVPY
Those candidates are having a science and mathematics or science /mathematics / biology stream after 10th can appear in this test. Candidates who are going to appear in this test after 10th should have 80% aggregate marks in science and mathematics in 10th and 1st year candidates must have 60% aggregate marks in 12th science and mathematics. There is a relaxation in percentage of SC/SC/OBC category candidates. For more details of eligibility criteria and other details Visit Eligibility Criteria for KVPY 2015. Applicants who are eligible for this test can apply online and offline mode. Candidates who want to apply offline can download an application form from the official website or can request for application form by post till last date in October 2015.
Important steps for apply offline steps
- Applicant want to visit the Official website of Kishore Viagyanik Protsahan Yojna (KVPY) at"kvpy.iisc.ernet.in/main/index.htm" on official website.
- Then applicant want to click on "Applications" button.
- Then applicant want to click on "Apply online" button and read the instruction.
- Then applicant want to Click on "Continue" button Option.
- Then applicant want to select your stream and enter the details.
- After that applicant want to click on submit Button for Application form.
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Sydney's favourite non-for-profit organisation Major Raiser put together unique campaigns designed to engage younger people with fundraising. To date, they've raised thousands of dollars for organisations like The Music Outback Foundation and Australian Youth Against Cancer. As part of their campaigns, they've become renowned for their parties, which have included acts like RüFüS, New Navy, Parades and Bon Chat, Bon Rat.
In 2013, they've re-launched solely as a charity that funds school feeding programs in impoverished nations around the world. Their GIVVAFORK (get it?) campaign has seen them partner with a number of Sydney restaurants to raise money for the UN's World Food Programme. These eateries will be stocking GIVVAFORKS throughout March, with all money going to the charity. $1 = four school meals provided to children in Laos – think about that as you settle your bill.
To celebrate the campaign, Major Raiser have put together their biggest gig yet. For just $15 (guess where the money's going!?) you'll get to check out an incredible line-up including Elizabeth Rose, Olympic Ayres, Polographia and special guests Van She.
As Major Raiser always say, come along and "Party with a purpose!"
WHEN: Saturday, March 30
WHERE: The Standard – Level 3, 383 Bourke Street, Surry Hills
HOW MUCH: $15 + bf. Buy tickets
RSVP: Facebook.com/events/188344521289234/
MajorRaiser.org.au | Facebook.com/MajorRaiser | WeAreTheStandard.com.au


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Get Your Dog Care Questions Answered by Experts
Hello. Thanks for writing in.
How long ago did this ingestion occur?
10 minutes or so
Thank you for the follow up. Lizi is doing fine. No adverse effects from the onions and I watch my plate closer now. Thank you for your assistance.


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Food Processing Schools in Virginia
The information below is designed to help people explore potential careers, and find information about colleges offering related educational programs. If interested,each individual may contact multiple schools.
Also try online schools for food processing, for more choices and flexibility.
Featured Food Processing Schools in Virginia
Kaplan UniversityCampus
Kaplan University is focused on recognizing the achievements of military and veteran students and offers the flexibility of an online education.
- Locations:
- Fort Lauderdale, FL
American UniversityOnline
Change the World with American University
Be a part of something bigger than yourself.
- Locations:
- Online
Southern New Hampshire UniversityOnline
- Locations:
- Online
California University of PennsylvaniaCampus
Recognized by the U.S. News and World Report for: Best Online Programs, Bachelor's 2017, Best Online Programs, Grad Education 2017 and Best Online Programs, Bachelor's for Veterans 2017
- Locations:
- California, PA
Northcentral UniversityCampus
Earn your graduate degree online with Northcentral University.
- Locations:
- San Diego, CA
Ashford UniversityOnline
- Locations:
- Online
Liberty UniversityCampus
100% Online & No Standardized Testing
- Locations:
- Lynchburg, VA


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20 Games Like Animal Crossing: Amiibo Festival()
Truth or Lies
In Truth or Lies, which is set for release this fall, players join family and friends in a roundtable style game play answering an astonishing array of thought-provoking questions. Utilizing a proprietary voice calibration system that works in conjunction with either the Xbox 360 Wireless Microphone or USB microphone, Truth or Lies measures stress levels in a player's voice to reveal the honesty of their answers.
Scene It? Box Office Smash
Scene It? Box Office Smash brings movie and trivia fans together for a game that provides hours of laughter as you challenge your friends and family to see whose movie knowledge reigns supreme reliving some of your favorite moments from the silver screen. Continuing the franchise's social and engaging trivia experience on the Xbox 360, Scene It? Box Office Smash features all-new questions, more high-definition (HD) movie clips and several new puzzle types, giving you a trivia game overflowing with images as well as audio and video clips from hundreds of films you know and love.
The Jackbox Party Pack 3
The threequel to the party game phenomenon features the deadly quiz show Trivia Murder Party, the say-anything sequel Quiplash 2, the surprising survey game Guesspionage, the t-shirt slugfest Tee K.O., and the sneaky trickster game Fakin' It.
97 / 10054.85
Drawful 2
The team behind the hit party games Fibbage, Quiplash, and YOU DON'T KNOW JACK presents Drawful 2, the game of terrible drawings and hilariously wrong answers!
You use your phone or tablet to draw weird and funny things like "pitcher of nachos" or "death by trombone." The other players type in what they think the (probably terrible) drawing is and those become the multiple-choice wrong answers. Then everyone - even an audience of potentially thousands – tries to guess the REAL answer.
Drawful 2 is a go-to party game that everyone can play and enjoy!
Scene It? Lights, Camera, Action
The popular DVD board game makes its first appearance on Xbox 360 with Lights, Camera, Action. Over 1,800 original movie-themed questions are available across two game modes: Play Now and Party Play. Packaged along with the game are four custom wireless controllers featuring large colored buttons so players can quickly submit or "buzz-in" a multiple-choice answer before their opponents.
Featured categories include Child's Play, where a child's artwork is used to depict a movie scene; Quotables, where part of a film's dialogue is missing; Puzzle Picture, which scrambles an actor's image; Invisibles, where actors and other items have been removed from a scene; and Clip Study, which has players answering questions about a short clip. The game automatically keeps track of which questions have been already asked to minimize repeats.
For 3-8 Players and an Audience of thousands!
The team behind the hit party games YOU DON'T KNOW JACK, Fibbage, and Drawful presents Quiplash, the laugh-a-minute battle of wits and wittiness! Use your phone or tablet to answer simple prompts like "Something you'd be surprised to see a donkey do" or "The worst soup flavor: Cream of _____."
No rules, no correct answers! Say whatever you want!
Your answer is pitted against another player's answer in a head-to-head clash of cleverness and comedy (or just "Which answer is least stupid?"). Other players – and even an Audience of people waiting to get in the next game – then vote for their favorite answer.
Quiplash is a go-to party game that everyone can play and enjoy!
Genital Jousting
Genital Jousting is an online and local multiplayer party game about flaccid penises and wiggly anuses for up to eight players at once.
93 / 10064.65
DJMAX Respect
DJMAX Respect is an upcoming rhythm action game primarily developed by Rocky Studio in co-operation with DJMax Sound Lab Team for the PlayStation 4.
Are You Smarter Than a 5th Grader? Game Time
Find out if you are Smarter Than A 5th Grader! Face Jeff Foxworthy's jokes as you try to answer more than 5,000 Game Time questions. Become a whiz kid in over 25 categories. Play like the show or try your hand in brand new Game Time modes including Three & Out and Schoolroom Showdown. Try your hand at Extra Credit mini games to boost your GPA. And if you think you're the smartest in the room, play against your friends and family in four-player challenges.
You Don't Know Jack
YOU DON'T KNOW JACK® - where high culture and pop culture collide!
Loaded with outrageous questions, wall-to-wall music and sound effects, blistering humor, and Cookie Masterson, your semi-lovable master of ceremonies, YOU DON'T KNOW JACK® is trivia-licious!
Key features:Up to Two players on the same computer
Genetically engineered with over 70 Episodes & over 15 hours of cranium-contorting questions.
Like that? Want more? Play favorite question types including DisOrDat™ and The Jack Attack™.
Long-lasting flavor with hundreds of questions that stay crunchy... even in MILK!
Taunt your opponents with more fun features like Screw Your Neighbor™ and the Wrong Answer of the Game!
Let the frivolity ensue with insane animation, hi-octane music, and hilarious wisecracks from your lovable yet verbally sassy host.
Lightning-fast gameplay – Don't drag your pants! The quicker you buzz in, the more cash you win or lose!
Super Smash Bros. for Wii U
Super Smash Bros. for Wii U is the fourth console-based entry in the popular Super Smash Bros. series of fighting games. With an even larger roster of fighters than its predecessors and the introduction eight player battles, Smash for Wii U continues to add to the formula that gamers have loved since 1999.
86 / 100524.3
Family Trainer Magical Carnival
Enjoy the theme park of your dreams without ever leaving home! Family Trainer Magical Carnival lets players explore an array of intriguing themed theme park zones including haunted houses, a lively circus tent, a pirate ship on the high-seas, a colorful parade and magical fantasy attractions. A veritable thrill-ride of activities will keep the whole family moving as they tame lions, steer pirate ships, explore haunted houses, ride a flying carpet and much more.
The Croods: Prehistoric Party
The Croods: Prehistoric Party! features the lovable Croods family: Grug, Ugga, Eep, Thunk, Sandy, Gran and Guy, on a hilarious adventure through 30 unique and addictive party-style mini-games. Explore spectacular landscapes in an incredible new world filled with never-before-seen creatures as you battle your friends and family in this awesome adventure with up to four friends in multiplayer gameplay.
Start the Party!
Start the Party!, previously known as Move Party, is a 2010 augmented reality party video game for the PlayStation 3, which utilizes the PlayStation Move controllers.[1] It was officially unveiled at the 2010 Game Developers Conference in San Francisco.[1] The game is the first game developed by Supermassive Games and was published by Sony Computer Entertainment for release as a launch title for the PlayStation Move and was bundled with it in Asian regions excluding Japan.[2] The game is a collection of augmented reality mini-games which utilise the PlayStation Eye and was released in Europe on 16 September 2010 and North America on 17 September 2010.
Viva Piñata: Party Animals
Full description
A multiplayer game expanding the Viva Pinata experience. Compete in a variety of zany battles and races in the social party game Viva Piñata: Party Animals. Control the fame, fortune, and destiny of all your favorite characters from Viva Piñata: Fergy Fudgehog, Paulie Pretztail, Franklin Fizzlybear, and Hudson Horstachio. It's party time!
Bomberman Online
Just when you thought Bomberman had planted his last explosive, the prolific video game hero returns for a modern update on his classic series. With addictive battles, frantic new modes, and brand new stages, Bomberman Online offers explosive action for all. Fans of the older multiplayer versions of the franchise will rejoice at the inclusion of four online modes Battle Royal, Hyper Bomber, Panel Attack, and Submarine Attack each designed to accommodate up to 10 players. The game's lone single-player mode boasts five awesome levels, and the graphics are rendered in 3D this time around.


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Product details
The Franke Galassia GAX 611 sink featured a sculpted, Italian styled drainer pattern and a large single bowl
- Material: Stainless Steel
- Effects and finish: Silk.
- Includes: Waste kit. 1 x basket strainer waste
- Guarantee: 50 Years
- Dimensions: (W)50, (D)19cm
- Type of sink: 1 bowl inset
- Orientation of sink: Right handed
- Dimensions of cut out: (W)48, (L)98cm
- Length: 100cm
- Usage: All
Franke Galassia 611 Kitchen Sink - 1 Bowl 885311
Select product options
Franke Galassia 611 Kitchen Sink - 1 Bowl
Available to order at the service desk in store for home delivery
Currently out of stock for home delivery
Franke Galassia 611 Kitchen Sink - 1 Bowl is rated out of 5 by 1.
Rated 5 out of 5 by Anonymous from as required Lovely sized sink really spacious, just hope that I never have enough dirty dishes to fill it! Very pleased with this product.
Date published: 2013-01-13


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Upcoming Events for Vertical Measures
July is busy month here at Vertical Measures. As you might already know, Vertical Measures is a founding member of AZIMA, the Arizona Interactive Marketing Association. Arnie is also the President, and Elise is the Administrator on the AZIMA board. Over the past three months, each of the board members have been working diligently to get AZIMA up and running, and preparing for upcoming events.
Tags: AZIMA, AZIMA events, e-commerce platforms, On-Site SEO, SEO events
About Kaila Strong
As Senior Director of SEO Services, Kaila oversees both the SEO department and our Internet Marketing Strategists. She works with our expert team to uncover SEO strategies, develop link building campaigns, conduct competitive analysis, review Google penalties, execute backlink analysis and train peers on SEO fundamentals. +Kaila Strong


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Let's try to keep this Mystery Car thing going. It's going to require a change to Facebook and it's going to take a little adjusting. Let's try it out here on the main site. Let's forget the Forum until we figure this thing out.
We've got a good little candidate here that needs to be identified. Can you tell us the year, make and model? ANSWER HERE by signing in to your Facebook account and commenting below. Will it work? Let's see.
This one should be easy -- deliberately, while we work on this little experiment.
Chris Woodyard is an auto writer for USA TODAY who covers all aspects of motoring. He revels in the exhaust note of a Maserati and the sharp creases of a Cadillac CTS. Chris strives to live a Porsche life on a Scion budget. More about Chris


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Video Wills, Kate & Harry Want A National Conversation On Mental Health
Men are less likely to talk than women with 54% of women having had a conversation compared to 37% of men.
Detectives are appealing for information following a shooting which left a 11-year-old girl and her mother injured.
Police were called at approximately 21:40hrs on Sunday 20 January to Chelsea Crescent, St Cuthberts Road, Cricklewood, following reports of shots fired at a residential address.
Officers and London Ambulance Service attended. Two females were discovered suffering from gunshot injuries and taken to a central London hospital.
In a statement, police said:
A 48-year-old woman suffered gunshot wounds to the chest and leg and her daughter, an 11-year-old girl suffered a gunshot wound to her foot. Their conditions are not described as life-threatening.
Four other adults were in the house at the time and they were not injured. All parties were known to each other.
Two black males were seen running away from the property at the time.
The Trident Gang Crime Command is investigating.
Enquiries continue and there has been no arrests so far.
Detective Inspector Glenn Butler as appealed for witnesses to come forward.
He said:
"This incident occurred in a residential area, which may have lead to a number of people seeing what occurred.
"If you saw anything unusual within Chelsea Crescent and the surrounding roads, then please contact the incident room. The motive for this incident remains unclear but I am keeping an open mind as to all possibilities."
Anyone with information on the shootings is asked to call the incident room on 0208 733 4774 or Crimestoppers on 0800 555 111.
Men are less likely to talk than women with 54% of women having had a conversation compared to 37% of men.
A career in the media and entertainment industry starts here...
A man has been fined £1,800 for flying drones over Parliament, near Buckingham Palace and over football stadiums.
Government to investigate after some restaurants accused of withholding tips
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About the Book
Once again, Ken's garden makes an impressive appearance, captured by my faithful Nikon D40x. Part one of two part series for 2009. Second part contains all my Passion Flowers & Angel Trumpets. (VF)
Features & Details
- Category Home & Garden
Standard Landscape, 10×8 in, 25×20 cm
120 Pages
- Publish Date Dec 03, 2009
- Tags Garden, Flowers
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On electrical transport in CoWO4 single crystals
- Received:
- Accepted:
- Citations
- 70 Downloads
CoWO4 is a p-type semiconductor with conductivity in the range 10−8 to 10−3 ohm−1 cm−1. The solid exhibits extrinsic behaviour up to 750 K and intrinsic behaviour above 750 K. In this solid conduction by hopping of small polarons seems to be dominating up to 750 K and above 750 K conduction becomes band type. The energy band gap of the solid has been found to be 2.80 eV. The values of mobility and the mean free path are estimated. The variation of dielectric constant with temperature has been attributed to changes in atomic and ionic polarization and space charge polarization of thermally generated charge carriers.
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© Chapman and Hall Ltd 1981


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PITTSBURGH - Dear residents of the Steel City, I have something I need to tell you: I'm sorry.
For several years now, I've had a burgeoning distaste for your town. I ignored all of the most-livable-city awards and the talk about how Pittsburgh had reinvented itself after the decline of the steel industry, focusing only on its stereotypical image as a smoggy, burnt-out town in the Rust Belt. I respected your sports traditions, especially the legacy of the Pittsburgh Steelers. But as a Green Bay Packers fan living in Washington who'd come across one too many Steelers fans that have trouble with either history or math (since the NFL began play in 1921, the Packers have 13 championships, more than double the Steelers' six), I ran your team up the list of franchises I love to watch fail. And I've spared few opportunities to take playful punches at the team, or the city, on Twitter.
But my most insidious offense - and the one for which I feel the greatest need to apologize today - is my violation of a principle tenet of baseball writing: Sample size matters. Or, as my mother might say, don't knock it until you've tried it.
You see, in four years covering the Nationals, I'd never visited your fair city. Instead, I'd allowed my image of it to be crafted by what I saw on TV or heard East Coast bluebloods say about it. When the travel schedule finally lined up for me to travel west this weekend for the Nationals-Pirates series, though, I decided I'd better get enough information to make a fair evaluation. So, resisting my urge to strut around town all weekend in my Super Bowl XLV champions swag, I set out to explore the city.
What I found, quite frankly, surprised me. First, your baseball stadium (PNC Park) is a gem, and it would appear your football stadium (Heinz Field) is, as well. The ability to get from downtown to both of them by foot or boat is tremendous. And your downtown area, as it turns out, is clean, walkable and full of places to eat. It's got both a sense of history and revival.
Finally, and perhaps most importantly, your town's commitment to hearty, greasy food is truly elite. I ventured with a few of the Nats beat writers to Fat Head's Saloon on Pittsburgh's South Side on Friday, and was thoroughly impressed with the high-end bar food and beer list. Saturday's trip was to the legendary Primanti Bros. Restaurant, which scored well in three areas: It had appeared on "Man vs. Food," it referred to soft drinks as "pop," not "soda" (I'm from the Midwest, I still call it pop and I'm not changing), and it adhered to the first Ben Goessling Principle of Sandwich-Making - a sandwich is not big enough unless there's stuff falling out of it with every bite.
As it turns out, Pittsburgh, in forming my short-sighted opinion of your city, I'd ignored many of the same virtues that life-long East Coasters miss when they denigrate my native Midwest as "flyover country." In a way, you've got the same artsy-meets-salt-of-the-earth feel that my hometown of Minneapolis has. And like Wisconsin, from which my entire family hails, your town is full of northern European descendants, Germans and Poles raised on back-breaking labor, family values and an intense love of their sports teams. It's probably not a coincidence that the Packers and Steelers, quite possibly the two greatest franchises in NFL history with the two most passionate fanbases, take their names from labor-heavy industries that have now largely gone away. Our paths involve the same roots, struggles and high points. We share a love of comfort food and kitschy phrases ("Yinz guys" for you, "You betcha" for us). We might produce the meat, cheese and bread for the burgers, but what would they be without Heinz ketchup? As president Abraham Lincoln said in his First Inaugural Address 150 years ago, we are not enemies, but friends. Or, in the words of another great dignitary, Rocky Balboa, if I can change, and you can change, everybody can change.
(Wait, he was supposed to be from Philadelphia, right? Is that a sore spot?)
So in conclusion, Pittsburgh, I'm sorry. I thank you for giving me a chance to have my stereotypes erased, and I look forward to spending more time in your fair city. I'm excited to walk around PNC Park this weekend to see what makes it one of the most highly-respected parks in the game. And maybe i'll get to Heinz Field someday, to see where the Steelers won their last game of the 2010-11 season.
(Of course, to see where the Packers won the last game of their 2010-11 season, I'd have to head another 1,200 miles south to Dallas.)
OK, OK, that was a cheap shot. But from what I can tell, Pittsburgh, you can appreciate a little good-natured teasing. I've learned enough about you to know I don't have any reason for digs more serious than that.


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Matrix Ping Pong
I frequent ebaumsworld to see unusual video. I came across this little gem of a video depicting a ping pong game. However, as you will see there is more than meets the eye!
The FCP are getting gearing up for the 2006 Conference, It will be held back at Eastern Mennonite University in VA. They don't have the total cost as of yet.
If you are interested you can contact the 2006 Conference Directors at:
Directors Gary and Ann email@example.com or go to their website at:
Workshop Leader CoordinatorConference Registration includes workshops, performances, exhibits, and special events; plus lodging for 5 nights and meals for Monday night thru Friday Night.
Tipi Tales is a 13 part, half-hour children's puppet series designed to illustrate the seven sacred teachings of Aboriginal spirituality to children by showing the importance of connection between children and their elders, man and his creator, and love of oneself. Four children face basic morality questions, or issues that need to be overcome, with the help and guidance of two grandparents and the legacy of the seven sacred animals that embody each teaching. As the moral dilemma is overcome, the children learn to rely on the values extended by their grandparents to empower themselves when facing obstacles. Tipi Tales can be found on APTN (Aboriginal Peoples Television Network) @ 9:00 AM, Wednesdays.
I came across this posting a couple of months ago, at the following link:
"Celebrate the Puppet"
A conference of the
Puppeteers of America: Great Lakes Region
and the
Ontario Puppetry Association
Fanshawe College, London Ontario
July 27 – 30, 2006
All information about the conference is located via link
Andrew, started his blog site in January 2004 and is a fellow Canadian. His blog's address is:
Andrew also has a PUPPETEER world map! Where you can post your puppet business or personal information. It is a great idea! You simply can make a faster connection with others in the way of networking, or if you are travelling and want to catch a performance just go here and see:
Andrew's site is what inspired me to start this blog, it is very well done and I highly recommend it! I would go to his Blog first, read everything, then go sign in on the map. Andrew states who he wants on his Puppeteer World map (which is located on his Blog) so if you fall in those guidelines, map away!
PS-I'm on the map too, I'm the groundhog located in Hamilton, Ontario.
Okay, here is a neat website I found concerning a company in
Sherman Oaks CA. http://www.swazzle.com they have included documentaries, and how to's when it comes to building puppets at a classroom settings. It is a good introduction to the processes of starting a puppet performance if you are starting a troupe and don't know anything about the production aspect(s). Enjoy!
I surf the net in search of anything dealing with puppetry, and came across other Puppetry Blogs. I found it very informative and wanted to follow the trend, so I'm starting this site in hope to reach anyone else like me and the other fellow bloggers to not only network but to put out information and promote puppetry!
Onward fellow puppeteers! Learn, Learn, LEARN!


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Hey guys,
What a nice moment of the day 6:21 P.M here in the Dominican Republic, to be grateful for the opportunity to be able to #WorkFromAnywhere. I'm here checking my #stats, writing some posts, and getting ready for a long night of work, guess where?? At my daughter's #VolleyballCamp.. And you?, what are you grateful for?


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Common Questions
The Session
Do I have to be in the pictures? Can't you photograph my dog/cat by himself?
We certainly pride ourselves in capturing the curiosity, quirkiness and humanity of our non-human companions, but David's real claim to fame is portraying relationships among family members – human or otherwise.
So, no, you don't have to be (linked to album with no people) in the pictures, but why not? (linked to album of people with their pets) You don't have to choose. David typically does both, and any other combination you dream up. It doesn't cost any more, and remember, you're free to select whichever images you love, and leave the rest behind.
What should I wear?
We recommend attire that makes you feel confident, comfortable and that expresses who you are! Solid colors work best, but avoid both BLACK and WHITE. Jeans, layers and textured clothing (denim, waffle weave, corduroy, linen) will add warmth to your photos. If you have questions, just call us. You might also like to bring several options and make the final decision here.
Please avoid shirts featuring logos, graphics or bold patterns. Pay attention to your shoes (and your children's shoes) as your feet will almost certainly appear in some of the final images. Your big snow boots or giant basketball sneakers, for example, might detract from the overall mood of the images.
How much time should I budget for my Sutton Studios experience?
We are not only warm, we're efficient! Though your sitting should last from 30-60 minutes, we'd like you to budget about 90 minutes with us on the day of your portrait sitting, a little more if you're coming with a large group. That's enough time for us to make our magic, and not so much that the more exciting species among us (ok, kids too) will become bored.
What will I need to pay?
When you schedule your appointment, you will pay us a nonrefundable $50 deposit to reserve your session time. On the day of your portrait sitting, you'll just pay the balance of your sitting fee.
We'll help you put your print order together during your second visit to our studio – the big day when you get to view your proofs. This occurs about a week after your sitting (and must happen within thirty days). (link to How long until I can see my proofs, and how do I select the photos I want to purchase?)
My dog is aggressive. Will there be other dogs there?
Don't worry, your dog is safe. We schedule portrait sittings with plenty of just-in-case time in between, so that animals who don't know each other aren't being introduced in our space.
We therefore ask that you arrive on time rather than early, for your appointment. Please do make us aware of any behavioral or aggression issues your animal may have so we can be sensitive to those concerns, and keep both your dog and our photographer safe.
My dog is nervous.
My dog is afraid of flash.
My dog won't sit still.
My dog isn't trained.
Is that a problem?
Few (if any) of our clients' companion animals are professionally trained. David has 20+ years of experience and he's worked with more than 10,000 dogs and cats (and there were even several special encounters with an alligator named Bubba.) For a recent philanthropic event, he photographed 89 of man's best friends in one day!
These dogs and cats have taught David effective techniques for working with a wide spectrum of animal personalities. Right behind liver treats, David's most important tool is his legendary patience. Though saintly is not the first adjective he'd use to describe himself, the most common feedback we receive after a session is "David has the patience of a saint." We couldn't make this stuff up if we tried!
Some dogs fear new places or new people, some are convinced that any trip outside the house is inevitably delivering them to a needle-wielding veterinarian, and some may be unhinged by the car ride that brought them.
David initially meets with your family (both human and non human members) in our reception area before beginning the photo session. He'll greet your dog/s/cat/s and talk with you to create a reassuring atmosphere for all. Once everybody is acquainted, he will walk you into the camera room. In the camera room, your pets will be given ample time to inspect the room and reassure themselves about the darker corners before we ask them to begin to work.
Should I get my dog groomed?
Grooming is a personal decision – you know how your dog will look her best – but please bathe your dog a day or two before your sitting. If you plan on having her groomed, we recommend setting the grooming appointment for a day or two before the sitting. A grooming and a portrait session on the same day can be a lot of stress for your dog, and we want her to be relaxed and very much herself for the photos.
A word about nails: Your groomer will probably want to trim your dog's nails for you, but we'd prefer they didn't. Those freshly trimmed nails can be pretty rough, and they're hard on our hand-painted backdrop and, at times, on our photographer.
How will you get my child to smile for the photos?
We live in a world where we have everything available on-demand: Chinese take-out, Netflix, shined shoes, groceries. What we sometimes fail to realize, however, is that most children cannot smile on demand. Even if they do, it will seldom be the kind of smile you want to display on your wall. In order to capture and highlight joy, there must actually be ... joy. That's what David will work on with your child.
While it may be challenging for you to sit in the wings and refrain from shouting "Say Cheese!" we'd be remiss if we didn't inform you that Sutton Studios is a cheese-free zone. David has been directing photo shoots for over 20 years and has a way with both children and animals. (link to the"never work with kids or animals" blog.) He adores them, and they feel it. He's even mastered the art of making funky noises to conjure the reactions he seeks. They may smile. They may not. Relax. We think you'll like your pictures (link to families album) either way. No cheddar required.
After the Session
Can I purchase high-res files and print them myself?
Here's the thing: our brand is rooted in a cultivated, definitive look, which we strive diligently to preserve. Each David Sutton original "out in the world" is our ambassador. David inspects and approves (or rejects and we go back to work) Every. Single. Print. Our process results in the consistency and incredible quality that our clients trust and expect.
So, can you purchase high-res files and print them yourself? Nope, but for good reason. We send each of our clients home with not only a smile and a homemade liver treat, but also fine-art-quality prints ready for display.
We deliver extraordinary customer service including listening to and embracing our clients' visions for the art they imagine, leaving nothing to chance. We employ personalized retouching services and our clients benefit from our exclusive relationship with our perfectionist lab that adheres to exacting processing standards and processes your final prints on the finest materials available in the medium. Oh, and we're control freaks.
But I got all the digital files from my wedding. . .
That's a horse of a different color. Wedding photographers use a different business model and customarily charge very high session fees up front, as part of a complete package that may include delivery of the digital image files (so clients can reproduce inexpensive, disposable copies for all the friends and relatives who want cake-face-smashing snapshots.)
By contrast, we charge a nominal sitting fee, which places the burden on David to create compelling, irresistible images.
You are never under any obligation to purchase prints. After you've reviewed your proofs and made your purchase, you can be confident that the artwork you're investing in has been handled by exacting professionals, every step of the way.
But I want to post the pictures on Facebook. How can I do that?
Easily! Once you have met with our Photo Design Experts and finalized your print order, we will place a selection of the selected images into an album on our studio's Facebook page (search: Sutton Studios Portraits). The image files are in .jpg format and bear handsome and discreet Sutton Studios logo.
These work great for all your social media and networking sites, while protecting David's copyright. You're welcome to circulate these electronic images as you see fit.
How long until I can see my proofs, and how do I select the photos I want to purchase?
Immediately following your portrait session, we'll schedule your print ordering consultation. This consultation will occur about a week after your sitting (and must happen within thirty days.) During your ordering consultation, we'll help you put your final print plan together.
You'll work closely with one of our Photo Design Experts who can talk you through your options (link to price list/video page) and help you achieve the artistry you're hoping for, whether you intend to create a gallery in your living room or make a bold, artistic statement on your wall complemented by a dramatic album or book. Oh, and don't forget personalizing your office space and making gifts for your more deserving friends and family.
Please be sure to measure your walls before your consultation. Consider your proportion guidelines and limitations so you're prepared to order appropriate and complementary sized prints.
Do keep in mind that we keep proofs active for 30 days from the date of your sitting. If we haven't received your final ordering decision within the first 30 days, your printed proofs may no longer be available to see. So, spit spot. Chik chak. Beat feet.
I like them ALL! Can't I just buy the proofs?
Sorry. Every photograph we deliver to our clients must earn David's approval, and our lab proofs simply don't make the cut. Our work proofs are neither retouched nor fine-tuned to our exacting specifications. They also experience a great deal of handling, which undermines their stability and longevity.
They're GREAT as a jumping-off point to help determine your needs and plan your displays and albums, but they're not meant to last, and they do not leave the studio.
When will my finished prints be ready?
Your finished prints will be available within 6-8 weeks of placing your order. During the winter holiday season (November/December), however, we compress our production cycle to accommodate your holiday gift giving needs.
Do you offer framing?
We have close relationships with professional framers who have been impressing our clients for years, and we're happy to provide you with all their information. Fine art framing is a specialty, like photography, and we believe it's best left to the experts.
Do you offer custom greeting cards?
Why, yes, we do! You can order greeting cards with photos from your session through one of our affordable, web-based greeting card associates (currently Minted.com and TinyPrints.com.) Pricing varies by product. We require a $50 per-image file prep and initial set-up fee; then you can order as many cards as you like directly from our online associates.
Will you be featuring my photographs in your promotional materials?
We'd love to; is that OK with you? On the day of your session, we will present you with a model release form. While you are absolutely not required to sign the release (we respect your privacy and will not ask questions about your decision) doing so allows us to use our favorite photographs in our exhibitions and promotional materials, as stock imagery, and in advertising. We exhibit in galleries and other performance-based spaces several times a year, and we look forward to inviting you to these exciting events.
My daughter's red hair is her best feature. Can I get one or two shots in color?
David's signature work is monochromatic. We painstakingly process the raw images and take them through an exacting multistep process with the ultimate goal of achieving the timeless black and white look for which David is famous.
That said, you may have a daughter with gorgeous auburn locks or a Weimaraner puppy with piercing blue eyes, and want to highlight and display these features. Although David's reputation has been built on black and white, we can indeed have a few of the images from your session re-rendered into color for you. Just ask. No hard feelings.
What if I have to reschedule?
We understand that some conflicts are simply unavoidable and we will try our best to work with your schedule. Should you need to move an appointment, we ask for one week's notice prior to your scheduled day, so we can cater to all of our clients and their needs. A $50 fee will be applied for reschedules within 7 days of an appointment.
Can you come to my house instead?
Most of our portraits sittings take place in our studio where we have optimum control over lighting, background and environment (control freaks, remember?)
However, David can indeed come to you, if you prefer, (link to an on-location video) and photograph your family in your home or the setting of your choice. Call us for details about our on-location fees.
I work all day. Can we do my session at night? On the weekend?
Appointments during standard business hours are most cost-effective for you, the client, but if an evening sitting or Saturday sitting is what you need, we'll make the arrangements. We want to work with you, so we'll do our best to plan your sitting within your scheduling constraints.
Do you do weddings and events?
Although David does not do event or wedding photography himself, our Production Manager, Daniela Cardili does, and we recommend her unequivocally. Dani has a photojournalist's reflexes and a portratist's eye, and she's well respected in the industry, all of which positions her as the ideal person to capture these momentous occasions.
Do you offer gift certificates?
We do indeed. We can work with you to design a sitting + print package that will fit both your budget and your gift giving needs. You can purchase your gift certificate over the phone or come in and make the arrangements in person. Call us and we'll talk you through it.


text: Okay, as you may know already, my wiki was deleted. I now have it on good authority (DaNASCAT) that indeed a group of immature children did complain about it. Turns out that they didn't like I had only three blogs that pointed out flaws in making a wiki into a dictatorship. Those kids decided to argue with me saying that I was wrong and uninformed to what they were actually trying to say. I pressed them further to inform me on exactly what they meant to say and they said, I quote: "not going to argue with a troll anymore". Heres my issue, I was not trolling, I was simply asking for them to back up their arguments. Did they? No. They turned to complaining when there was no reason to. And because of their immature actions, I no longer have a way to bring my fanbase closer together. All that work I did, gone because of a bunch of immature kids. Was I in the wrong? Yes, I should of never even bothered with kids who run a wiki that is full of drama and abuse. Are they in the wrong? They are 100% in the wrong. They complained to have a wiki they didn't like get shut down. Now, I have nothing. They win, and Wikia Staff has yet again allowed those immature kids have their little spoiled victory. No other wiki complained about me, yet when a wiki that shouldn't even be cared about (Players Wiki) complains, red flags always have to go up. Seriously Wikia Staff? Go ahead, argue with me, say I'm overeacting, in the end, you will agree that you're letting a bunch of spoiled brats run around causing drama on Wikia. I stand by the facts in this situation. If you wish, I can provide evidence to why it is Players Wiki that should be shut down, and I will have even more of that to show from other wikis, because my wiki isn't the only wiki that's had problems with Players WIki.