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Evolution of specifier proteins in glucosinolate-containing plants
Genome evolution and evolutionary systems biology
The glucosinolate-myrosinase system is an activated chemical defense system found in plants of the Brassicales order. Glucosinolates are stored separately from their hydrolytic enzymes, the myrosinases, in plant tissues. Upon tissue damage, e.g. by herbivory, glucosinolates and myrosinases get mixed and glucosinolates are broken down to an array of biologically active compounds of which isothiocyanates are toxic to a wide range of organisms. Specifier proteins occur in some, but not all glucosinolate-containing plants and promote the formation of biologically active non-isothiocyanate products upon myrosinase-catalyzed glucosinolate breakdown.
Based on a phytochemical screening among representatives of the Brassicales order, we selected candidate species for identification of specifier protein cDNAs. We identified ten specifier proteins from a range of species of the Brassicaceae and assigned each of them to one of the three specifier protein types (NSP, nitrile-specifier protein, ESP, epithiospecifier protein, TFP, thiocyanate-forming protein) after heterologous expression in
Among specifier proteins, NSPs represent the ancestral activity. The data support a monophyletic origin of ESPs from NSPs. The split between NSPs and ESPs/TFPs happened before the radiation of the core Brassicaceae. Future analyses have to show if TFP activity evolved from ESPs at least twice independently in different Brassicaceae lineages as suggested by the phylogeny. The ability to form non-isothiocyanate products by specifier protein activity may provide plants with a selective advantage. The evolution of specifier proteins in the Brassicaceae demonstrates the plasticity of secondary metabolism within an activated plant defense system.
A distinguished feature of plant secondary metabolism is its enormous diversity. More than 200 000 secondary metabolites are known to date that are either widely distributed in the plant kingdom or specific for a small group of plant taxa. Evidence is accumulating that this chemical diversity is shaped by selection pressures exerted by the biotic and abiotic environment of the plant
Figure 1
Glucosinolates and glucosinolate hydrolysis.
Glucosinolates and glucosinolate hydrolysis. A. Examples of structures of aliphatic glucosinolates (1, 4-methylthiobutylglucosinolate), aromatic (i.e. Phe-derived) glucosinolates (2, benzylglucosinolate), and indolic glucosinolates (3, indol-3-ylmethylglucosinolate). B. Using allylglucosinolate (4, aliphatic side chain with terminal double bond) as an example, the hydrolysis by myrosinase (a) is shown that leads to the formation of an isothiocyanate upon spontaneous rearrangement of the aglycone (b) or to the formation of alternative products (c-e). The ability to form organic thiocyanates depends on structural requirements of the glucosinolate side chain and the presence of thiocyanate-forming protein (TFP; c). Epithiospecifier proteins (ESPs) promote the formation of epithionitriles upon hydrolysis of glucosinolates with a terminal double bond (d), but do not support organic thiocyanate formation. Simple nitriles are formed at acidic pH, in the presence of ferrous ions or due to the action of nitrile-specifier proteins (NSPs; e). If a glucosinolate does not fullfill the structural requirements for epithionitrile formation, ESPs promote the conversion of the aglycone to the simple nitrile. In a similar way, TFPs support epithionitrile or simple nitrile formation upon myrosinase-catalyzed hydrolysis of glucosinolates that do not allow organic thiocyanate formation.
In the glucosinolate-myrosinase system, chemical diversity is generated by independent variation of glucosinolate biosynthesis and glucosinolate breakdown
To date, nine plant specifier proteins with different substrate and product specificities have been identified at the molecular level and characterized biochemically, namely the epithiospecifier proteins (ESPs) from
Here, we identified specifier protein cDNAs from a range of species of the Brassicaceae that showed their ability to form non-isothiocyanate products in a phytochemical screening among representatives of the Brassicales order. As the amino acid sequence identity proved not to be sufficient for the assignment of a given specifier protein to one of the three types (NSP, ESP, TFP;
Results and discussion
Phytochemical screening
To study the occurrence of non-isothiocyanate products of glucosinolate hydrolysis within species of the Brassicales, we analyzed 21 species of 14 genera of the Brassicaceae as well as seven species from five other Brassicales families for their glucosinolate and glucosinolate hydrolysis product profiles (Additional file
Additional file 1
Table S1. Glucosinolate classes and hydrolysis product types in species of the Brassicales. The plant organs indicated were analyzed for glucosinolates by HPLC after derivatization to desulfoglucosinolates. For the analysis of glucosinolate hydrolysis products, fresh plant material was homogenized in aqueous solution, and dichloromethane extracts of the homogenates were analyzed by GC-MS. Glucosinolates were assigned to structural classes according to the structure of their side chains as follows: A1, aliphatic: methylthio-/methylsulfinyl-; A2, aliphatic: terminal double bond; A3, aliphatic: terminal double bond and 2-hydroxy-group; A4, aliphatic: other than A1-A3; B1, benzyl-; B2, 4-hydroxybenzyl-; B3, 2-hydroxy-2-phenylethyl-; B4, Phe- and Tyr-derived other than B1-B3; C, indolic. Glucosinolate hydrolysis product types are abbreviated as follows: I, isothiocyanate; N, simple nitrile; EN, epithionitrile; OX, oxazolidine-2-thione; T, organic thiocyanate. An X indicates the identification of at least one representative of a glucosinolate class or a hydrolysis product type in the given plant material (n.i., not investigated). Most species were of the Brassicaceae. For species of other families, the family is given below the species name.
Click here for file
Additional file 2
Figure S1. Glucosinolate hydrolysis products in
Click here for file
Identification and characterization of specifier protein cDNAs
Brassicaceae species that produced simple nitriles, epithionitriles or organic thiocyanates were used to identify specifier protein cDNAs by a PCR approach (with the exception of
Additional file 3
Figure S2. Alignment of previously known specifier proteins for primer design. Protein names are as given in Table 1. Amino acid sequences were aligned with the ClustalW option implemented in MEGA 5.05
Click here for file
Additional file 4
Table S2. Oligonucleotide primers for cDNA isolation. Sequences are given starting with the 5' end. Mixed nucleotide codes are: D, A + G + T; K, G + T; M, A + C; R, A + G; S, C + G; W, A + T; Y, C + T. Protein names are as given Table
Click here for file
Additional file 5
Figure S3. Assignment of newly identified specifier proteins to the ESP, TFP or NSP type. Activity was tested using crude extracts of bacteria expressing specifier protein cDNAs, 1 mM allylglucosinolate (A-K) or 2 mM benzylglucosinolate (L-V) and 0.005 units myrosinase in 50 mM MES buffer, pH 6.0. Shown are total ion current traces of GC-MS chromatograms recorded of dichloromethane extracts of the assay mixtures after 30 min incubation. Products of allylglucosinolate breakdown are simple nitrile (1), organic thiocyanate (2), isothiocyanate (3), and epithionitrile (4). Products of benzylglucosinolate are simple nitrile (5), and isothiocyanate (6). (X) indicates the peak of the internal standard. The following cDNAs were used: A, L, no cDNA (empty vector control); B, M,
Click here for file
For seven of the eleven Brassicaceae species subjected to the PCR approach, specifier proteins were identified (Table
Phylogenetic analyses
Together with specifier proteins identified in previous studies, a total of 19 characterized specifier proteins representing nine genera of lineage I and expanded lineage II of the Brassicaceae according to
Additional file 6
Table S3. Amino acid and nucleotide sequence identities of specifier proteins/ specifier protein cDNAs. Amino acid sequence identities are shown on the right, nucleotide sequence identities on the left as determined in separate comparisons of each sequence pair by ClustalW implemented in MEGA Vers. 5.05.
Click here for file
Additional file 7
Figure S4. Phylogenetic tree of specifier proteins from Brassicaceae. Full-length amino acid sequences of 19 biochemically characterized NSPs, ESPs and TFPs (Table 1) as well as three putative specifier proteins (Table 3) and one homolog of unknown function (At3g07720) were subjected to phylogenetic analysis using the Maximum Likelihood algorithm with 1000 bootstrap repetitions.
Click here for file
Additional file 8
Figure S5. Neighbor joining tree of specifier protein cDNAs from Brassicaceae. Full-length nucleotide sequences of 19 biochemically characterized NSPs, ESPs and TFPs (Table
Click here for file
Additional file 9
Figure S6. Maximum Parsimony tree of specifier protein cDNAs from Brassicaceae. Full-length nucleotide sequences of 19 biochemically characterized NSPs, ESPs and TFPs (Table
Click here for file
Figure 2
Phylogenetic tree of specifier protein cDNAs from Brassicaceae.
Phylogenetic tree of specifier protein cDNAs from Brassicaceae. Full-length nucleotide sequences of 19 biochemically characterized NSPs, ESPs and TFPs (Tab. 1) as well as three putative specifier proteins (Tab. 3) and one homolog of unknown function (At3g07720) were subjected to phylogenetic analysis using the Maximum Likelihood algorithm with 1000 bootstrap repetitions. Bootstrap values are given at the nodes. A homolog from
In four species, pairs of specifier protein paralogs were available. Each pair consisted of one NSP of the AtNSP1 cluster and one ESP. Thus, for each functionally characterized NSP of the AtNSP1-cluster, an ESP paralog could be included in the analysis. Pairwise analyses of the amino acid distances (
Additional file 10
Table S4. Amino acid and nucleotide distances of specifier proteins/ specifier protein cDNAs. Amino acid distances are shown on the right, nucleotide distances on the left as determined by MEGA Vers. 5.05 using the Equal-input + G model (
Click here for file
Time estimates date the beginning radiation of the core Brassicaceae at 40–50 million years ago, i.e. at about the same time as the
A closer analysis of the ESP/TFP-cluster revealed that specifier proteins of this cluster form several subgroups. A first branch contained only two ESPs identified from
As alignment gaps are considered non-informative in the above analyses, the results represent the phylogenetic relationships based on comparisons among the kelch domain regions of the sequences analyzed. Therefore, we performed a separate analysis of those regions of NSPs that are JAL domains. The two JAL domains of AtNSP4 were used separately with the N-terminal JAL domain termed A and the second JAL domain termed B. The analysis confirmed an earlier study on AtNSP1-AtNSP4 and suggested a monophyletic origin of all JAL domains except JAL domain A of AtNSP4 (Figure
Figure 3
Phylogenetic tree of JAL domains of NSPs from Brassicaceae as well as homologs composed of JAL domains identified in databases.
Phylogenetic tree of JAL domains of NSPs from Brassicaceae as well as homologs composed of JAL domains identified in databases. Amino acid sequences of JAL domains of eight NSPs (marked with a black circle) and 26 homologs of unknown function from
Analysis of selection pressure
Nucleotide substitutions in coding sequences can either be silent (synonymous) or amino acid-changing (non-synonymous). Natural selection mainly acts on non-synonymous substitutions leading to different rates of non-synonymous vs. synonymous substitutions
In order to analyze selection pressures acting on specifier protein genes, we determined non-synonymous vs. synonymous substitution rate ratios (ω) using pairwise comparisons of full-length coding sequences (Additional file
Additional file 11
Table S5. Non-synonymous vs. synonymous substitution rate ratios of specifier protein cDNAs.
Click here for file
As pointed out by Kroymann (2011)
While the toxicity of isothiocyanates, whose formation does not require specifier proteins, and their role as direct chemical defenses have been proven in various studies
Phylogenetic analyses of ten newly identified and nine previously known characterized specifier proteins of the Brassicaceae as well as three putative specifier proteins from Brassicaceae found in databases and one homolog of unknown function suggest NSPs to be the ancestral representatives of this group of proteins and provide evidence for a monophyletic origin of ESPs from NSPs. The split between NSPs and ESPs occurred before the diversification of the core Brassicaceae, i.e. at least about 36 million years ago. TFP activity evolved from ESPs later, and phylogenetic analyses indicate that this happened independently in different lineages. Based on the data available to date, specifier proteins might be an innovation of the Brassicaceae that likely expands the defensive repertoire of the Brassicaceae, among others possibly as a response to selection pressures exerted by specialist herbivores that evolved adaptations to the glucosinolate-myrosinase system. The evolution of specifier proteins demonstrates the plasticity of secondary metabolism in the context of an activated plant defense system.
Chemicals and general experimental procedures
Custom-made oligonucleotides were from Invitrogen. Allylglucosinolate (sinigrin) was purchased from AppliChem. Benzylglucosinolate was purified from
Plant material
Sources of seeds are given in Additional file
Additional file 12
Table S6. Sources of seeds for growing plants for phytochemical analysis and cDNA isolation.
Click here for file
Glucosinolate analysis
Plant material was frozen in liquid nitrogen, stored at −80°C, and freeze-dried before analysis. Glucosinolate profiles were determined by HPLC of the corresponding desulfoglucosinolates as described previously
Glucosinolate hydrolysis products in tissue homogenates
Fresh plant tissue (100–300 mg) was homogenized in 500 μl 50 mM MES buffer, pH 6.0. After 5 min incubation at room temperature, 50 μl internal standard (phenylcyanide, 100 ng μl-1 in MeOH) were added, tissue remainings were removed by centrifugation, and the supernatant was extracted twice with 750 μl CH2Cl2. Samples were concentrated in an air stream to about 200 μl and analyzed by GC-MS as described
cDNA preparation and degenerate oligonucleotide PCR
Leaves were frozen in liquid nitrogen and stored at −80°C until used for cDNA generation. Total RNA was extracted using ribozol-OLS (OLS OMNI Life Science) or innuPREP Plant RNA Kit (Analytik Jena) according to the manufacturer's instructions and used for reverse transcription with SuperScript III Reverse Transcriptase (Invitrogen) and oligo(dT)12–18 primers (Invitrogen) according to the instructions of the manufacturer. Based on an amino acid sequence alignment of known specifier proteins, conserved regions within NSP sequences and within ESP/TFP sequences were identified (Additional file
Gene-specific primers (Additional file
Cloning of full-length cDNAs and heterologous expression
Sequence information of the fragments obtained by RACE-PCR was used to design gene-specific sense and antisense primers for amplification of the full-length cDNAs or ORFs, respectively (Additional file
Specifier protein assays
Assays were carried out in a total volume of 500 μl 50 mM MES-buffer, pH 6.0, containing 1 mM benzylglucosinolate or 2 mM allylglucosinolate and crude bacterial extract corresponding to 1.5 mg total protein and were started by addition of 0.005 units of myrosinase. After 30 min incubation at room temperature, 50 μl of phenylcyanide (100 ng μl-1 in MeOH) were added as internal standard and the mixture was extracted twice with 750 μl CH2Cl2. Organic phases were pooled, dried over Na2SO4, concentrated under an air stream to about 200 μl and analyzed by GC-MS as described
Data base search
Blastn was used to search the nucleotide data base at NCBI using cDNA sequences of known specifier proteins as queries. Hits were included in the phylogenetic analyses if they represented complete ORFs with an identity of at least 60% with those of characterized specifier proteins. Using
Sequence and phylogenetic analyses
Motif and pattern searches were done with InterProScan at
NSP: Nitrile-specifier protein; ESP: Epithiospecifier protein; TFP: Thiocyanate-forming protein; JAL: Jacalin-related lectin.
Competing interests
The authors declare that they have no financial and no other competing interests.
Authors' contributions
JCK performed phytochemical analyses, cloned and characterized specifier proteins, and carried out phylogenetic analyses. MB performed phytochemical analyses. UW designed the study together with JCK and MB and analyzed data. UW wrote the manuscript with comments from all authors. All authors read and approved the final manuscript.
Tobias Wille, Loretta Heise and Anita Backenköhler are thanked for excellent technical assistance and Burkhard Bohne and Ina Meier for help with obtaining seeds and growing plants. We thank The Botanical Gardens listed in Additional file
From waste products to ecochemicals: Fifty years research of plant secondary metabolism
Natural diversity and adaptation in plant secondary metabolism
Repeated evolution of the pyrrolizidine alkaloid-mediated defense system in separate angiosperm lineages
Seeing double: Gene duplication and diversification in plant secondary metabolism
The glucosinolate-myrosinase system in an ecological and evolutionary context
Terpene synthases and the regulation, diversity and biological roles of terpene metabolism
Functional plasticity of paralogous diterpene synthases involved in conifer defense
Differential levels of insect herbivory in the field associated with genotypic variation in glucosinolates in Arabidopsis thaliana
Methylthioalkylmalate synthases: Genetics, ecology and evolution
Regulatory networks of glucosinolates shape Arabidopsis thaliana fitness
Glucosinolate biochemical diversity and innovation in the Brassicales
The chemical diversity and distribution of glucosinolates and isothiocyanates among plants
Biology and biochemistry of glucosinolates
Role of glucosinolates in insect-plant relationships and multitrophic interactions
Myrosinase: gene family evolution and herbivore defense in Brassicaceae
Localization of plant myrosinases and glucosinolates
Role of camalexin, indole glucosinolates, and side chain modification of glucosinolate-derived isothiocyanates in defense of Arabidopsis against Sclerotinia sclerotiorum
Glucosinolates, isothiocyanates and human health
Positive selection driving diversification in plant secondary metabolism
Glucosinolate hydrolysis in Lepidium sativum - Identification of the thiocyanate-forming protein
Crambe thioglucoside glucohydrolase (EC 184.108.40.206): separation of a protein required for epithiobutane formation
Biological effects of glucosinolates
The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia ni herbivory
The gene controlling the quantitative trait locus EPITHIOSPECIFIER MODIFIER1 alters glucosinolate hydrolysis and insect resistance in Arabidopsis
Glucosinolate hydrolysis and its impact on generalist and specialist insect herbivores
Tipping the scales - specifier proteins in glucosinolate hydrolysis
Glucosinolate breakdown in Arabidopsis - mechanism, regulation and biological significance
Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane
A thiocyanate-forming protein generates multiple products upon allylglucosinolate breakdown in Thlaspi arvense
The genetic basis of constitutive and herbivore-induced ESP-independent nitrile formation in Arabidopsis
The kelch repeat superfamily of proteins: propellers of cell function
Antagonistic jacalin-related lectins regulate the size of ER body-type beta-glucosidase complexes in Arabidopsis thaliana
Cabbage family affairs: the evolutionary history of Brassicaceae
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana
Large-scale collection and annotation of full-length enriched cDNAs from a model halophyte. Thellungiella halophila
Thiocyanate formation from glucosinolates: a study of the autolysis of allylglucosinolate in Thlaspi arvense L. seed flour extracts
The synthesis and enzymic hydrolysis of (E)-2-[2,3-2 H2]propenyl glucosinolate: Confirmation of the rearrangement of the thiohydroximate moiety
The intramolecular formation of epithioalkanenitriles from alkenylglucosinolates by Crambe abyssinica seed flour
MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance and Maximum Parsimony Methods
Dated molecular phylogenies indicate a Miocene origin for Arabidopsis thaliana
Arabidopsis family ties: Molecular phylogeny and age estimates in Brassicaceae
Genome duplication and the origin of angiosperms
Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models
Comparative biochemical characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolates
Formation of simple nitriles upon glucosinolate hydrolysis affects direct and indirect defense against the specialist herbivore, Pieris rapae
Indole-3-acetonitrile production from indole glucosinolates deters oviposition by Pieris rapae (white cabbage butterfly)
The genetic basis of a plant-insect coevolutionary key innovation
Isolation of sinigrin and glucotropaeolin from cruciferous seeds
Altered glucosinolate hydrolysis in genetically engineered Arabidopsis thaliana and its influence on the larval development of Spodoptera littoralis
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

subset: commoncrawl


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Ron Nessen (Ronald Harold Nessen)
BiographyWashington, District of Columbia, USA
Ron Nessen was born on May 25, 1934 in Washington, District of Columbia, USA as Ronald Harold Nessen. He is a writer, known for The Presidents (2003), Paul Conrad: Drawing Fire (2006) and Follow the Leader (2002). He is married to Johanna Neuman. Read more... <


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The convenient lift out tray with special convoluted foam to protect your handguns, sits on top for safe transport and easy access. The double latching system prevents accidental opening and helps seal the box from dust and moisture. There are also two pad lock tabs for added security. The large comfortable handle makes carrying the case to and from the range comfortable and easy.
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I'm pretty sure I am done. I'll still hang around and stuff, for a while. But, I think I am done trying to figure things out.
I had a discussion with Dion on my Septenary Man thread, and it is crazy when I look at my old writings, writings more than 20 years old of mine. Fuck. I've accomplished them. I was on a quest to touch my soul, my spirit, and see what I was comprised of.
Throughout my entire life there has been an ache, a longing, to struggle through all the many illusions of what we are before we have been entangled in the web of the human condition.
The human condition is comprised of unreality. It is comprised of errors and distortions and longing and blindness, and false hopes and deception and the worst of it; relying on others to find the self. Co-dependence and crutches and fantasies and all the many, many disturbing, debilitating constructs of fear and doubt.
I have asked the questions throughout my life, since the time I can remember: What are we and what are we here for?
Even when these answers become known, there is no relief or release like we think there will be. Unfortunately, learning or suspecting the answers...well the answers remain
beyond human expression. It is akin to trying to describe, in full and in perfect form beyond equivocation , the reason why you love your child, or the reason you were born. Read the tales of the saints.
link to en.wikipedia.org
Read of St. John of the Cross and Saint Teresa of Avila. Their experiences, according to modern day Christians on this site would be of demonic influence.
Ah, I am getting off on a tangent.
There is no 100% absolution. I know now why the religious find such comfort in believing something from outside themselves will provide full absolution. How/why? Because it cannot be done save for fantasies and beliefs and the power of conformity that chains the soul.
The chains, the restriction, the boundaries that the human condition has constructed about itself...are comforting. We have been indoctrinated, composed of since our first breath, the predisposed human fallacy of not cycles, but of a singular beginning and end.
It all needs to be redone. The holy wars, the separation, the judgements, the righteousness. There is a problem though. Before, these things were isolated ideologies. Now, they consume the world. Not to mention the vices of greed and vanity.
We are due a cleansing. Cleansing does not mean death, but it does mean a painful SCRUB; a painful clearing of things attached to us that are not of our own self.
ALL the things you experience in this life are your own...
~ I need to get back in touch with myself. I have strayed a very long way from my center. It is difficult being this sensitized to the world, to the people surrounding my life.
I want to go deep, deep down into the thing that I am. Touch my soul and release it. Let it flow out and consume my subjective being.
I can sense it here. I feel it. It's just out of sight. It feels strange. It is here. And it is me. But it feels like someone else.
I hope one day I allow it to span this chasm of fear and sorrow that I have dug. Let my soul cross it, and like the sands beneath the ocean waves, I will let it engulf me and let it preside over the confusion and doubts that have constructed my life.
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Genre: Comedy In the follow-up to the record-breaking hit comedy "The Hangover," Phil (Bradley Cooper), Stu (Ed Helms), Alan (Zach Galifianakis) and Doug (Justin Bartha) travel to exotic Thailand for Stu's wedding. After the unforgettable bachelor party in Las Vegas, Stu is taking no chances and has opted for a safe, subdued pre-wedding brunch. However, things don't always go as planned. What happens in Vegas may stay in Vegas, but what happens in Bangkok can't even be imagined. Rated: Unrated Directed By: Todd Phillips XviD | English | avi | 608x256 | XviD @ 760 Kbps MP3 @ 128 Kbps | Genres: Comedy | Romance Uploader Note: When 1gb version will be available will be uploaded also if you want better quality in bigger size Note:If you have something to offer as early releases,seedboxes, links,accounts,etc please contact us on torentz 3xforum ro Seed Stats: 20-400 GiB at aprox 10 MiB/sec/20 slots per torrent How To Get Your Maxspeed (uTorrent) Torrent Client - Preferences - Bandwidth - Number of connections - Set aprox. 500 for each Torrent Client - Preferences - BiTorrent - Protocol Encryption - Forced Bandwidth Allocation - High (Set download/upload limit - Unlimited) Stop Other Active Torrents - Start downloading and wait for connections. Enjoy and Seed for at least 1:1 ratio. Don't forget to say thanks/leave reviews. Before downloading you should check/google search sample "release name""sample". Greets to all actual/future p2p/scene QUALITY groups and affiliate websites. Don't trust IMDb robot or bad ratings,always watch some trailers first. Ignore stupid comments/fake/mislabeled/no-DVDrips/CAM/TS/RAR uploads. Please spread this release/torrent to your friends/forums/etc. If you need translation subs visit some subtitles sites. Report if you see online streaming or other spam links. Recommended movie player VLC PLAYER.
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History of the OberthEdit
The USS Oberth (NCC-630) was constructed subsequent to the Hermes-class Scouts as a Survey Class Science Vessel. This ship was later refit and re-designated NCC-602 during a Starfleet-wide registry re-designation. The original ship to bear that number, the USS Aries, was destroyed in the line of duty. As the Oberth was almost completely rebuilt from the keel up, the Oberth NCC-602 is considered a new build, and first of the Oberth-class.
Fan Film InformationEdit
Author's notes: I created this ship in an attempt to avoid de-canonization of the Hermes-class. NCC-602 is a conjectural number for Oberth, as it was never seen on screen, but it would supersede the USS Aries which was published in 1975. Hermes-class has been seen in canon reference in the Star Trek films. The above explanation is intended to make it easier for all references to remain intact and still work together so that everyone's interpretations can coexist. The ship as seen above will appear in two episodes of Starship Saladin.


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Mix together a marinade of lemon, oil, oregano, and garlic. Add meat and let marinate for 2 to 3 hours in the refrigerator. Remove meat from the marinade and thread on skewers. Place on a hot grill, turning a few times, for 10 to 15 minutes.
Recipe courtesy of Ann Pappas


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vril starts living above her means,sending Letitia to a private school and Jackson is horrified to learn she has been financing it by embezzling the Jockey's holiday fund. Vilified by the estate she goes to work for Lillian but Jackson's overtime and Letitia's scams pay off the money owing. Mimi meets fellow jogger Neil and is soon enjoying a sexual relationship but dumps him when she learns he prefers her to gain rather than lose weight. Chesney is concerned that delivery driver Gorky,separated from his wife,is abusing Bean,his 12-year old daughter and sets out to frame him for drug-dealing.However Bean takes drastic action of her own and Chesney helps her to escape.Resumen IMDb.com
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Heineken Cup match preview: Clermont ring the changes for trip to Wales
Clermont Auvergne will be looking to make it six wins from six in Pool 5 when they travel to face the Scarlets.
Last Updated: 19/01/13 5:23pm
The Welsh side have yet to rcord a win in Europe this season, including going down to a 49-16 loss against their next opponents at the start of the competition.
Head coach Simon Easterby has opted to move Rob McCusker to number eight with Sioni Timani starting at blindside flanker, whilst Johan Snyman comes into the second row.
Exciting prospect Samson Lee starts at tighthead prop, meaning South African Jacobie Adriaanse drops to the bench.
Clermont, meanwhile, know a win in Wales would make certain of a home quarter-final. They have yet to taste defeat in the group stages, including a 46-3 thrashing of Exeter last time out.
The Heineken Cup favourites have made no less than eight changes from the side that put the Chiefs to the sword, including handing a start to Wales international Lee Byrne at full-back.
Julien Malzieu comes in on the right wing with Benson Stanley partnering captain Aurelien Rougerie in the centres. David Skrela is the other change in the backs, coming in at fly-half for Brock James.
In the pack, Raphael Chaume starts in place of Thomas Domingo, with Alexandre Lapandry and Elvis Vermeulen joining Julien Bonnaire into the back-row.
Clermont reached last season's semi-finals only to be knocked out by eventual winners Leinster, who are still hopeful of making it out of the same pool this year.
Scarlets: 15 Liam Williams, 14 George North, 13 Jonathan Davies, 12 Adam Warren, 11 Kristian Phillips, 10 Aled Thomas, 9 Tavis Knoyle, 8 Rob McCusker, 7 Josh Turnbull, 6 Sione Timani, 5 Johan Snyman, 4 George Earle, 3 Samson Lee, 2 Ken Owens, 1 Phil John.
Replacements: 16 Matthew Rees, 17 Rhodri Jones, 18 Jacobie Adriaanse, 19 Richard Kelly, 20 Aaron Shingler, 21 Gareth Davies, 22 Gareth Maule, 23 Andy Fenby.
Clermont Auvergne: 15 Lee Byrne, 14 Julien Malzieu, 13 Aurélien Rougerie (c), 12 Benson Stanley, 11 Napolioni Nalaga, 10 David Skrela, 9 Morgan Parra, 8 Elvis Vermeulen, 7 Alexandre Lapandry, 6 Julien Bonnaire, 5 Nathan Hines, 4 Jamie Cudmore, 3 Davit Zirakashvili, 2 Benjamin Kayser, 1 Raphael Chaume.
Replacements: 16 Ti'i Paulo, 17 Vincent Debaty, 18 Daniel Kotze, 19 Julien Pierre, 20 Damien Chouly, 21 Ludovic Radoslavjevic , 22 Kevin Senio, 23 Regan King.


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Chad Riddle
- 67 years old
- Date of birth: Aug 28, 1947
- Place of birth:
Missouri, United States
- Date of passing: Apr 6, 2015
- Place of passing:
Missouri, United States
|Let the memory of Chad be with us forever
This memorial website was created in memory of our loved one, Chad Riddle, 67, born on August 28, 1947 and passed away on April 6, 2015. We will remember him forever.
Memorial Tributes
"My brother was one to listen then share his thoughts. He told you like it was, I admired him for that. He carried a heavy burden but never stopped trying to live life and share its meaning. I miss him and hope he's around me this Christmas season!!"
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And then a couple weeks ago, I got some exciting news that I had been chosen to be a part of 2014 Blogger Squad for Country Chic Paint!
I'm so excited to partner with these ladies and Country Chic paint as we all take our own spin on breathing new life into old pieces, and tackling new creations.
Plus, you'll be seeing a lot more before and after's from me!
Once this rain ceases at least. ;)
And in the meantime, I'd love for you to check out all the other contributors both on their blogs and our Pinterest Board.
Jane from Mamie Jane's
Melissa from While It Rains
Rachel from Maison de Pax
Ronda from The Everyday Domestic
Lindsay from My Creative Days
Krista from The Happy Housie
Jen from Migonis Home
Sarah from Life on Virginia Street
Tricia from Little Cottage on the Pond
Stacey from Embracing Change
Heather from Inspire Me Heather
DeDe from Designed Decor
Carissa from Carissa Miss
Happy {rainy} Tuesday!


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text: Dr. Ling was Brian Griffin's acupuncturist. In "He's Too Sexy for His Fat", he treats Brian to eradicate his fleas. Although he appears Chinese, he speaks with an Irish/Scottish accent. He is also unable to read English as he keeps his acupuncture needles next to a box of poison needles. Upon Brian's discovery of this, he ends his patronage with Ling.


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Ready for your first adventure Wildstar
Who plays ancient scrolls in the recent who know what adventure of the launch area in craglorn Wildstar forgive, but plans to do something different. The risk of five players familiar in the new design similar to a dungeon, but it is not linear.
"We're driving very history, is not difficult from the point of view of war to other copies of the game, but there will be many options and different styles of" Frost revealing. "One example is like an adventure you, where it says that the orphanage fire or someone drowned somewhere. That man is a very Important information of drowning. You can choose," Well, I like orphans and children I will save the children, other people drowned. If you want to play, you can choose baa.a, "Who cares about the orphanage. I have to go ahogamiento.así offered this option, you can play, it is the effect of seeing things.
"Also has several game types: one thing is probably a Moba, the desert war, for example. The other, galleys, like a tower of Defense in his defence, the military attack of military domination or exile. You want your life is the tower, once these fly, you lose or bad track, and a team of people throughout the region in the surface or assault team live element, such that the risk of cosas.así is crazy, is crazy." are you ready for the next adventure Wildstar.


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Pros: Look very solid, well made, I tried it on several bottle types. Ideal gift for your outdoorsy friends. Cons: I think it might be cheaper, but still good.
cons: carabiner is not really great, that's why only 4 stars pros: everything else as it should be tried to carry a 1,5L bottle, doing well
-Pretty useful -Keeps your hands free and pockets dry when carrying a cold bottle of water. -Build quality is not bad. -Does not fit all types of water bottles -Should make adjustable sizes Anyone who saw me with this was... Read more
Very cheap, but has very high-level quality. It can handle plastic and glass bottles. Carbiners are big enough and quality is much higher than I could even expect. I am very satisfied with this product. Really! Package to... Read more
Very useful to hang a bottle at your backpack or somewhere else. Easy tu use, to detach and place back. Can be used with standard PET bottles. When you turn the clip in other dirrection as it should be in my opinion, it be... Read more
Well, they just clip to your soda bottle, water bottle or what ever. Tested them with 2 litre pepsi bottles and they kept clip tightly. I... Read more
Pros: Look very solid, well made, I tried it on several bottle types. Ideal gift for your outdoorsy friends. Cons: I think it might be... Read more
cons: carabiner is not really great, that's why only 4 stars pros: everything else as it should be tried to carry a 1,5L bottle, doing well
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More than just a few years ago, I wrote about NFL quarterback Peyton Manning who, at the time was the all-everything leader for the Indianapolis Colts. I mentioned how I liked his coolness under pressure and even though he wasn't the quarterback for "my" team, I couldn't help but admire his calm demeanor and ability to analyze problems and adjust on the fly.
That is something that every produce manager can relate to.
Manning worked through some near career-ending injuries, and in March of 2012 ending up signing with "my" team after all (Thanks Indy). Broncos fans everywhere were excited. The question lingered, why did the Colts owner Jim Irsay cut Manning loose to begin with?
The popular thinking was Manning was damaged goods and releasing him meant creating salary-cap room to sign the talented but untested rookie Andrew Luck.
Perhaps it was the wise move or was necessary for Indy to preserve the franchise. Perhaps the Colts feared an aging Manning might not last a series, much less a whole season. They couldn't afford to keep him, most said.
I say they couldn't afford to let him go.
Football talk aside, I see this thinking a lot in the produce aisle, the pressure to hire a manager with less experience. Or a produce manager setting up a new store might be directed to hire a mix of talent: A few with a lot of experience, a few with some experience and a boatload with no experience.
Just like the football salary cap, this is due to an ever-shrinking labor budget. The produce manager is asked to train the underlings from scratch.
If this is the only available mix, so be it. However, experience counts for so much in the produce business. When I was in charge of staffing new or remodeled stores, we always hired experience first before plugging holes in the schedule with rookies.
It only made sense, especially when the doors opened for business and the customer rush was on, but the philosophy works in normal labor settings too. Especially when it came time to promoting someone to fill a new produce manager position.
Experience doesn't necessarily mean the number of years on the job. We looked for people who worked in varying levels, inner-city and suburban settings, slow and busy stores, covered vacations and was most familiar with the latest marketing trends and technology.
So when a chain instills this kind of produce manager in the ranks, they should do everything they can to develop and retain them. As they get stronger, the confidence builds. They can be turned loose to merchandise and manage freely.
Just like Manning, running that famous, no-huddle offense.
Armand Lobato works for the Idaho Potato Commission. His 30 years of experience in the produce business span a range of foodservice and retail positions.
What's your take? Leave a comment and tell us your opinion.


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Wednesday, August 3, 2005
VivoMetrics Adds to Advisory Board
Ventura-based VivoMetrics announced this week that it has added Arthur R. Bartolozzi, M.D. to its scientific advisory board. Bartolozzi is Chief of Sports Medicine at the The University of Pennsylvania Health System's Pennsylvania Hospital. VivoMetrics develops the LifeShirt System, a wearable garment used for monitoring respiratory and cardiopulmonary functions. The company's product are used by academic researchers, pharmaceutical companies, and others to monitor life signs and for clinical research.


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Dream Decorative Pillow
Sweet dreams indeed. This whimsical pillow is reserved for a loved one near or far, a sentimental reminder without the sappy love stuff.
This product is no longer listed on FindGift.com by Kohl's. This usually means that the product is sold out or has been discontinued. Check the Similar Gifts section above to find other gifts that may be suitable. Or, you can see more gifts from Kohl's


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McGuigan Black Label Rose 11.5% 750ml
Provence is an iconic, picturesque region of rolling hills, medieval villages, fields of lavender and is also the spiritual home of French Rosé. This wine is made in the traditional Provencal style, using Sauvignon Blanc and Grenache, resulting in a Rosé that is full of sweetness but balanced with great acidity. The Grenache is used to extract maximum floral notes from the skins, while the Sauvignon Blanc provides the delicate acidity on the back of the palate. Drink while young and fresh.


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Mel B: 'LA has so many overweight people'
Mel B recently sat next to Charlize Theron at a SoulCycle class.
Mel B doesn't feel inspired to be healthy when in Los Angeles because she views Americans as "the most overweight, obese people".
The former Spice Girl has been living Stateside on-and-off for years, but it hasn't altered her outlook on health and fitness. The star is renowned for being in shape so doesn't need any help with exercise, although she does enjoy the variety of food on offer in the City of Angels.
"No, they're the most overweight, obese people in America, but LA does have great alternatives if you want to be vegan or vegetarian or lower calorie, macrobiotic or the raw food. They have so many different options," she told Britain's Heat magazine.
Exercise-wise the star has bought into the LA lifestyle though and she's a regular at SoulCycle classes. These are static bike routines which take place in dark rooms with loud music - and you can guarantee other stars will be there too.
"I was sitting on a bike a couple of weeks ago next to Charlize Theron, and Puff Daddy was at another class. Everyone's there!" she laughed.
Mel enjoys experimenting with her diet now and then, sometimes with better results than others. Jay Z and Beyoncé famously shed pounds when they embraced veganism, but Mel didn't see any major benefits when she gave it a whirl.
"I went vegan recently, just to see what it was like and I missed chicken and steak too much, so after two weeks, I was like, 'OK, I've done that now,'" she said. "No (I didn't lose weight), but I slept better. That's about it. I guess I had a little bit more energy, too."
During her Spice Girls heyday the star was famed for her impressive six pack, which still makes an appearance now and then. She doesn't obsess about it these days, meaning sometimes she's more ripped than others.
"Just work out, eat lean and clean and get good rest," was her advice for a washboard stomach. "It's about being a little bit strict in looking after yourself. When I'm working out to my full potential it's all about running and weights. I work out every day for about 45 minutes."
© Cover Media
Belfast Telegraph Digital


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Israel News
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American Politics
Edition française
Green Israel
Saeb Erekat 311 AP.
(photo credit:AP)
Around 10 EU countries are set to upgrade the status of Palestinian
representative offices in their capitals in the near future, chief Palestinian
Authority negotiator Saeb Erekat declared on Sunday.This would mean that
Palestinian missions would move a step closer toward becoming embassies whose
officials enjoy full diplomatic immunity.
RELATED:South American countries recognize Palestinian stateMy Word: South American absurdity
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Being Reasonable About Religion Ebook
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Picky is an application catered for people residing in Singapore who are scouring everywhere for favourable restaurants. This application guides and recommends restaurants according to user's personal tastebuds and mood.
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There has always been a debate over the appropriateness of judicial review.
This may seem to imply that judicial review no longer serves a legitimate purpose and should be abandoned.
Rather than overturn Roe v. Wade, he proposes to overturn the entire system of judicial review.
But more than 200 years later, the disruptive potential of judicial review remained on full display.
According to the Quran, the highest political authorities are subject to judicial review.
This decision greatly strengthened the theory of judicial review of national legislation.
Before the principle of judicial review was supported by a single authoritative decision, it had thus become a partisan issue!
His aim has been to pass in judicial review the thoughts and imaginations of mankind concerning the destiny of the human soul.
He stated in one breath the principle of judicial review and the doctrine of enumerated powers.
Calderón restored the lands to their rightful owners and his act was approved in judicial review by the audiencia.
The principle by which courts can declare acts of either the executive branch or the legislative branch unconstitutional. The Supreme Court has exercised this power, for example, to revoke state laws that denied civil rights guaranteed by the Constitution. (See also checks and balances.)


text: 1,411 Quite Interesting Facts to Knock You Sideways is guaranteed to elicit a "who knew?" from even the most rabid trivia hounds. Each page features roughly thematically linked morsels of intriguing knowledge, distilled into a pithy sentence or two. Did you know, for instance, that 5% of cats are allergic to humans? The symbol for the female sex (?) represents the hand mirror of the Roman goddess Venus? Or that, after first meeting him, Mussolini described Hitler as a "mad little clown"? The authors tell us that everything has been meticulously researched - and that they've mercifully left all that boring stuff out. Why 1,441 you ask? The very first factoid presented is "There are 1,411 tigers left in India." We're also offering the authors' equally amusing two previous troves of eclectic informational tidbits. What fun and enlightening browsing for, well, anyone interested in anything! Also see 1,227 Quite Interesting Facts to Blow Your Socks Off and 1,339 Quite Interesting Facts to Make Your Jaw Drop. (AG)


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I was in a parking garage with 2 other people. 1 was gonna 'get something out of my car real quick".walked over opened a car with a key and removed a backpack and ipod. While handing the backpack to his girlfriend,she dropped it and several items fell on the ground.I helped her pick them up and she put them in her purse. Last thing was some folded up papers I picked up and hurried onto the elevator. Turns out... Not his car, nor his stuff. I was in the casino playing 6 hours later, security came and got me to speak to a sheriff . I gave him the papers and told him how I got them. He arrested me for496(A)Felony, the guy who committed the theft, admittedly, was cited for 484. Msdmnr Petty theft despite 6 priors, 3 for theft & current probation. I have no priors. How does that happen??Also, the arrest/evidence report shows the value of the items I was in possession of as $0. I said "that was his car, he had the keys to it" from the start, so how did I possess 'KNOWN" STOLEN PROPERTY??? **** The thing I most don't understand is this...If EVERYTHING he stole adds up to be less than $400 resulting in a Misdemeanor Petty Theft, how can a small fraction of it, some pieces of paper valued at $0 constitute a Felony??
Penal Code section 496 is a "wobbler", meaning it can be charged as either a felony or a misdemeanor. If the police have an option to arrest and book you on a felony, they will, since the number of felony arrests the generate factor into budgets, etc.
Will you be charged with a felony? With no record, it's unlikely. Will you be charged at all? That depends. It's up to the DA to review all the relevant reports and decide if they believe your "I didn't know" story vs. they think you knew and just lied to the police.
Either way, you'll need a good criminal defense attorney in your corner to protect you. Start your search NOW. They can try to make sure any video surveillance footage is preserved if it could exonerate you.Ask a similar question
496 pc requires that at the time of the incident you knew or reasonably should have known that the items were stolen. This does not appear to be the case hear. Thus, they would have a very difficult time proving their case.Ask a similar question
It certainly sounds that at the very least, you have a winable case. The DA is the entity that decides what you get charged with, if at all. It is imperative that you have a lawyer ready to represent you ASAP. Often times an attorney can talk to the issuing DA prior to charges being filed. This can sometimes prevent charges altogether or perhaps have them filed as a misdemeanor instead of a felony. However, in the event felony charges are filed, you certainly need to have a lawyer fighting for you and assuring that you are not going to be convicted for something that you should not be. This is a crime that requires very specific elements be met in order to be guilty. You should sit down with your lawyer and develop a strategy for defense immediately.Ask a similar question
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text:
Two Wheel Distance
762/762 Meters
A lot of this challenge will be completed during power slides, but for the remainder of the challenge, you can take a car to the freeway and drive halfway up on to the barrier that line that sides. If you drive too fast, you can get struck on it or even drive right over it, so get two wheels up on the barricade and then take your time driving across the highway. It will only take a few times to complete the challenge this way.
If you are following the "destroy 25 tanks" method for the other challenges, then this will come along with it. If not, go piss off a lot of cops for a tank to show up, take it and shoot at everything in sight. Almost every time you hit a vehicle it will be a multikill and with the wide AOE of the tanks artillery, almost every shot towards two people will be a multikill as well.
Basejumps Nailed
15/15 Landings
Base jumps are a pain in the ass, to be frank. When you jump out of a plane or helicopter, or jump off a high building, you can pull out your chute and then press Y/Triangle to set a marker on the ground to land in. Go to the airport and in the northeast corner there are two helicopter pads. Grab the copter on the right and take it up to about 8 seconds in the air. When you jump out, and chute and place the basejump marker, it should be around 80 meters below you. This height was the easiest for me to nail it each and every time. Land in the marker, anywhere inside the lights, and it will count. If your helicopter didn't survive the fall- sometimes it does- run back up the stairs to the helipad and another should have spawned by now for you to take up again. 14 more times and you can get this challenge out of the way.
No-Weapon Kills
25/25 Kills
This is one of the rather simple challenges to get. All you need to do is run up to 25 people without a weapon in hand (no Guns, no Penetrators, no Apoca-fists) and punch 25 people to death. If they go down and don't die, stand over them and attack to stomp on them to finish them off.
Stoppie Distance
305/305 Meters
This is another one of the more easy challenges. Grab a motorbike or scooter and head to the airport runways. Once there, get up to full speed and then slam on the brakes with the left trigger and push forward on the left stick to raise the back end of the bike. Continue rolling back and forth along the run way and you'll nab this easily in no time.
Stag Commandos Killed
25/25 Killed
During the campaign, after STAG has arrived and before the end of the campaign, is when you need to get this challenge. Cause a ruckus enough for Stag to arrive and get it to level 5 for the commandos to show up. If you want to bring the fight to them, head to Sunset Park in the Northeast corner and go nuts to bring them running immediately. If you try to do this once the game is completed, Stag won't show up, just the SWAT and cops will.
Compliments Paid
50/50 Compliments
There are no rules for the targets for this challenge thankfully. Simply walk up to 50 different people, civilians or Saints gang members and press right on the D-Pad to pay the person a compliment. Do it 50 times and you're done with this.
Distance Travelled
762,000/762,000 Meters
This will take a dang long time to get. Even with all the driving of the campaign, it will take some additional driving afterwards to nail the full total. One way to help increase the distance travelled is to hop in the VTOL and simply fly back and forth over the city. Once you hit the far side, the VTOL will turn around automatically and head the opposite direction, allowing you to not even worry about having to control it, or even pay attention to it.
EMU's Destroyed
50/50 Emus
Emu's are the tiny Smart Car type vehicles that you'll see driving around every once in a while. One way to destroy 50 of them rather quickly is nab one and then bring it to your garage at the HQ. Pull it out and then drive a couple feet and hop out. Blow it up and repeat. Another way is to wait for later in the game when the gang of mascots attack during a survival cellphone call. The mascots will arrive in both Emu's and golf carts giving you plenty to destroy to reach fifty.
Gang Ops Defeated
32/32 Gangs
There are 32 gang operations throughout the city of Steelport and to take over each part of the city, you'll need to defeat each in the various territories. If you haven't found all of them, look for the grey areas of the map and the map legend will tell you how many there are left to find. Once you have found them all, you can defeat them insanely easy by just having the VTOL fire a burst of missiles at them from the air.
Survival Completed
26/26 All Waves
This is easily one of the more time consuming challenges. Each time your cell phone rings, you'll have the opportunity to complete a survival challenge. You'll need answer the phone and then head to the location. There are two types of challenges; wave based where you need to kill a set amount of enemies per wave and wave based where each wave is time based and you need to survive long enough to make it to the end. You will only receive the phone calls while you are outside of a vehicle and walking.
Kill Brutes
30/30 Brutes
Just like the "destroy 25 tanks" challenge, piss off enough cops and gang members so that the tanks start rolling in. Hijack a tank and then use it to destroy everything in sight. During the rush of enemies that will continually appear, there will be a load of Brutes that arrive. Also using the tank, you can destroy their truck before they even get out making it all that much easier.
500/500 Headshots
Depending on how good your aim is, you may or may not need a lot to finish off this challenge by the time the main campaign ends. Either way, you can head to Arapice Island and climb on top of a car or a crate and let the zombie swarm all around you. Simply shoot downwards and chances are you'll always hit their heads. Let another group form up and rinse and repeat until you hit 500.


text: ~Ecosystem Portfolio~~African Savanna~ ~Biotic~~Lions~~Grass~ ~Trees~~Gazelle~~Rhino~ ~Abiotic~~Dead Tree~~Dead Grass~~Water~~Air~~Rocks~ For the population to survive they need grass , gazelle , water buffalo , rhinos , hippos , crocodile , and lions. If the population does not have these things one thing could die and another can over populate. Limiting Factors:the factor that prevent a population from growing any larger. Limiting Factor: dry season Predator/Prey Relationship:lion and water buffalo Producer: an organism that creates its own food. Ex:Grass Herbivore: An animal that feeds on grass and other plants. Ex: Gazelle Gazelles Diner by Amine Fassi Omnivores: An organism that eats both plants and animals. Ex: Baboon Monkeys Life by Arnulf F Carnivores: An animal that eats flesh. Ex: Lion A Lion Called Ron by David Lloyd Decomposers: An organism, usually a bacterium or fungus, that breaks down the cells of dead plants and animals into simpler substances. Ex: Beetle Hirschkäfermännchen by Leo Pöcksteiner Scavenger: An animal or other organism that feeds on dead organic matter. Ex: Vulture vultures squaring off by Brent Stapelkamp Producer Explanation: A producer converts radiant energy into chemical energy for herbivores. Ex: A tree Nairobi Boarder Lands by Ryan Benson Food Chain: A series of organisms interrelated in their feeding habits Food Web: A combination of food chains that integrate to form a network Removing a Population: If you remove one organisms population from the ecosystem, all other organisms will be affected. Ex: Over population or extinction An energy pyramid is a pyramid or triangle shape because the energy flows up. Only 10% of the energy flows up at each level. Producers are on the bottom of the pyramid because they make their own food and they do not need other plants and animals to survive.


text:
|Sasha Roiz
|Biographical Information
|October 21, 1973
Tel Aviv, Israel
|The Godfather II (video game)
|Agent Henry Mitchell
Sasha Roiz is a Canadian actor who was born in Israel. He has appeared in a number of popular television dramas, including CSI: Miami, House, NCIS, The Mentalist, Lie To Me, and Terminator: The Sarah Connor Chronicles and notably Caprica. He also portrayed CIA Agent Henry Mitchell in The Godfather II (video game).
Characters voiced by Sasha RoizEdit
The Godfather IIEdit
External linksEdit
- Sasha Roiz at the Internet Movie Database
- Sasha Roiz on Wikipedia


text:
This Tournament was made possible by the partnership with Las Plumas High School Thunderbird Robotics and Butte College Drafting Department. Under the leadership of Jon Andrew (LPHS) and John Dahlgren (Butte College), the high school robotics team hosted the event on their campus and judged the robotics portion of tournament while the Butte College students mentored small groups students in the engineering challenge of the day.
The students had a great experience. The Expanded Learning Department looks forward to continuing our mission to offer these sorts of unique opportunities to students in Butte County. See Summer Schedule.
Thank you again to those who participated and those who supported this event for our students!
Teams from following after school programs participated and won these awards:
2nd Place Runner Up, Most Gracious Professionalism
Most Inspirational
3rd Place Runner Up
Tournament Champion
Best Teamwork
Berry Creek
Engineering Judge's Award
For more information contact:
Mary Ellen Garrahy
Program Manager
Expanded Learning
Butte County Office of Education
1859 Bird Street
Oroville, CA 95965
Phone: 530-532-5647
Fax: 530-532-5699


text:
| Clip Of Energy and Foreign Policy This clip, title, and description were not created by C-SPAN.
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October 23, 2012
October 23, 2012
Sec.Hillary Clinton - Energy Diplomacy and Climate Change2012-10-18T21:07:08-04:00https://images.c-span.org/Files/857/20121018210743002_hd.jpgSec.Hillary Clinton on energy diplomacy and "the very real threat of climate change."
Sec.Hillary Clinton on energy diplomacy and "the very real threat of climate change."
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- Georgetown UniversityGeorgetown University
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text:
Disclaimer: I own nothing. Not even the plot bunny that attacked me….. *holds up arm, showing evil bunny attached to arm* Let's read the tags…. This plot bunny was set upon me by CANDYisEpic. Thanks for that…
A/N Finally! The long awaited one-shots have arrived! In chapter two of my story Teach Me How to Fly, I asked for prompts from reviewers. If you haven't read that yet, you probably should, but it isn't really needed. I received three prompts from reviewers, this one featuring Lucifer.
Oh, and read till the end—it gets better than the beginning.
Summary: Lucifer still seethes in his Cage, but after a surprise visit from one he thought was dead, he realizes that perhaps things are much simpler than he thought.
Lucifer buried his head in his arms, allowing his rage to completely take over his mind. Green eyes flashing in malicious intent, he spread his magnificent wings and roared, his normally calm, smooth demeanor shattered. Sam was gone, Michael was gone, Adam was gone, and he was alone once again. The sheer cosmic energy radiating off of him scorched the walls of his burning prison. Of course, it did nothing to free him. Michael built this prison, and as much as it pained him, Michael was much too perfect to make a mistake like that.
When he was first cast down, the pain was overwhelming. As an archangel, he had never felt true pain. But in Hell, there was no discrimination. All were punished. The most painful thing, however, was Michael's face swimming around in his vision. The cold brutality etched on his perfect features, the narrowed eyes as he cast his own brother down. But… hidden in those hazel eyes was a flicker of something.
Shaking the image out of his mind, Lucifer renewed his frenzied attack on his prison. Digging his fingers into the burning stone, he pulled. He pulled and ripped at the stone walls, only to have the stone shift back into place, neatly ordering itself into an impenetrable wall. Screaming in rage, he slammed a mighty fist into the wall, sinking down to his knees.
"Well, well." Lucifer whipped around to see the owner of the snarky voice staring back at him, prison suddenly forgotten.
It couldn't be.
"Hello, Luci," Gabriel said, a ghost of a smile on his face. Lucifer took a hesitant step towards his little brother, one hand outstretched to touch his face. He retracted it instantly, shaking his head in disbelief. It was new trick of Hell. That was it. Designed to torture him with his sins. Shooting a glare at his supposed little brother, he still couldn't help but feel his heart break. He hadn't intended to… it had all happened so fast…
Gabriel laughed his usual warming laugh, spreading his arms to the sides, "It's really me, Lucifer," he said, "In the flesh. Or," he paused thoughtfully, "Whatever it is we're made out of."
Lucifer searched his brother's face for any hint of remorse, any shred of anger, but found none. He hesitantly stepped forward, as if his brother was an image in the mist, to be blown away by any sudden disturbance. With a quick, hope filled step, he embraced his baby brother, holding him close as if he were the most precious thing in the world. Gabriel stiffened, then relaxed, returning his brother's embrace with a smile that, for once, was not a smirk.
"You… Father… did he…?" Lucifer stuttered, his usual eloquence forgotten momentarily.
"Yep," Gabriel answered brightly, "Dad brought me back. And better than ever, I might add," he said, shooting him a knowing wink.
Lucifer paused for a brief moment before asking, almost sheepishly, "…and… your Castiel?" he asked, averting his eyes.
Gabriel stiffened, "I…" he trailed off, looking away, "I haven't visited the kid yet. For all he knows, I'm still dead." Seeing his little brother in such pain, Lucifer felt even more remorse than he had felt in a very long time. Castiel meant more than anything to Gabriel, save Balthazar. Gabriel never was one to pick favorites. He himself, of course, was the exception. Lucifer knew that he was Gabriel's favorite elder brother.
"I remember when he was just a little fledgling who had flight problems…" Gabriel sighed nostalgically, "Always so protective, even then. I guess it came back to bite him with the Winchesters."
Lucifer turned to look at his little brother, "I couldn't save Uriel," he confided brokenly. His little brother that he had raised, and he had failed to protect him. Anger for Father welled up once again in the ex-archangel. Trying in vain to bottle it up, Lucifer trembled with utter fury.
"It's not like that, you know," Gabriel said quietly.
"What?" Lucifer snapped angrily, "It's not like Father doen't even care about what happens to us anymore? We were His firsts! We should be the ones He's watching over!"
"It's not like that," Gabriel repeated, "It's much more simple."
"How?" Lucifer asked bitterly, refusing to meet his younger brother's eyes, "Father hasn't even shown his face since I left. He knows that what I said was true. And look what our home has become," he sneered, "a prison camp to train new soldiers."
Gabriel's face showed the first flicker of anything but calm. "It's like that because you left," he ground out, obviously struggling to keep his ever-present temper in check, "They decided to make perfect little soldiers because they were scared of you. Of your choice." Lucifer was silent. Of course. It was just like Michael to smooth out pain by doing everything—humane or not—to make sure that it didn't happen again.
"And the humans, well," Gabriel shot him one of those "I-know-so-don't-even-try-to-lie-to-me" looks, "You have to know after taking two vessels, one of them being Sam Winchester, that they're not so different from us." Lucifer said nothing. Yes, he had been rather fond of the child Sam Winchester. He was so compassionate, so understanding, yet so filled with anger… just like him
"And Father?" Gabriel continued, much calmer now, "I don't think He's been hiding." He got up, walking to the door of the Cage and pressing a golden, lined wing to it. The air shimmered, showing an exit.
"He's been waiting." Turning around, Gabriel began to disappear into the shimmering air.
"Wait!" Lucifer blurted out, Gabriel turned around, looking at his brother with a classic smirk. "Don't…" Lucifer started, "Don't leave me alone again," he finished meekly. The Bright Morning Star subdued at last.
"Oh I'm not," Gabriel said quietly. "I'm leaving the door open." Lucifer stared, dumbstruck for the first time in his life. "I told you Father was waiting," Gabriel said, "He was waiting for you, Luci. His wayward son come home. You have a choice now, Lucifer." And with that, he left, the door of the Cage still open, as promised.
Lucifer stood there for what felt like eternity. Father was waiting for him? Father wanted him back? He felt the first cool breeze slip through the door, caressing his face, calling him, beckoning him.
A choice.
Stepping out into the cool night air, Lucifer spread his magnificent wings under the silver moonlight.
"I'm coming home, Father," he whispered.
Gabriel sat, keeping vigil of his little brother, Castiel, from Earth as he always did. His heart broke for the little guy, really. Keeping the peace in Heaven wasn't easy several millennia ago, and it sure as hell wasn't easy now. Especially with Raphael competing for power. But even try as he might, he couldn't bring himself to intervene. Some inane, sixth sense told him that his little brother had to grow up, couldn't always be leaning on him for support. But he still watched.
Lucifer appeared in a pile of limbs in front of Gabriel. But… it wasn't Lucifer. His brother had not a shred of Grace left, his wings just empty shadows.
Lucifer staggered to his feet, "Cursed coordination," he muttered, "how do they do it?"
"Uh, hello?" Gabriel called, waving a hand in front of his brother's face.
"What?" Lucifer snapped angrily, brushing himself off.
"What…." Gabriel gestured helplessly at his brother with both hands.
"What?" Lucifer repeated, "I'm tired, I'm hungry, I have this strange pressure in my lower stomach, and that landing hurt," he rolled his shoulders experimentally.
"You're.." Gabriel started.
"Yes," Lucifer sighed, "I've been 'reborn.'"
"I'm human."
The End
I'm evil. That's all I gotta say. :)
Please review! Unless you want Raphael to come and smite you in your sleep…. What?


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Winn-Dixie is a supermarket chain that sells all types of groceries. The supermarkets are located in the Southeastern United States with stores in Florida, Alabama, Mississippi, Louisiana and Georgia. Cashiers, managers and stock persons are just a few of the job positions offered. If you want to apply for a job at Winn-Dixie, you can do so in person, or you can submit your application online and wait for Winn-Dixie to contact you.
Visit the Winn-Dixie website and click on the "Employment" link on the home page. On the following page, choose whether you want to submit an online application for retail, distribution center, pharmacy or corporate positions, and click on the corresponding link.
Enter your Zip code, choose the number of miles you are willing to work from your home, and click the search button. Choose the store or office location where you want to work from the list of available choices.
Click on the "Apply Now" link. Read the terms and conditions, clicking "Next" when you are finished reading each page. Decide whether you want to create a pass code, which will enable you to access your application for 48 hours.
Answer each of the questions on the online application and provide all of the required information. You must provide your Social Security number, full name, address, contact information and resume.
Review your information. Make any necessary changes or corrections, and submit your application to Winn-Dixie. Once your application is reviewed, Winn-Dixie personnel will contact you regarding your resume and application.
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text:
Platts – Oil production from the Organization of the Petroleum Exporting Countries (OPEC) totaled 30.01 million barrels per day (b/d) in November, down 290,000 b/d from October, and down for a second consecutive month as Libyan production went into reverse after several months of steady gains, according to the latest Platts survey of OPEC and oil industry officials and analysts.
The November level compared to October output of 30.3 million barrels per day and is within a few hundred thousand barrels of the 30-million-b/d ceiling that OPEC, at its recent end-November meeting, decided to maintain despite multiple projections that such production could create a significant supply overhang in the market.
Libyan production had climbed to an average 860,000 b/d in October, almost double the July level. But a new security-related shut-in at the Sharara field in early November resulted in a month-on-month drop of 210,000 b/d.
"This is the great irony of the OPEC decision to leave its production ceiling unchanged," said John Kingston, Platts global director of news. "The status of Libya remains uncertain, with enormous swings of output that are measured in the hundreds of thousands of barrels per day. So an OPEC decision to stand pat could actually play out with unintended cuts – or production increases – from a still unstable Libyan situation."
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Microix, Inc.
A developer of business management software.Speak with a Microix, Inc. provider
Microix, Inc., (pronounced "mike roy") is one of the fastest growing software companies to provide reliable extended solutions to Best Software Sage Fund Accounting. Microix, Inc., specializes in the development of Microsoft Access, SQL Server and .NET solutions for the non-profit sector.
Our number one priority is to deliver feature rich and flexible products that provide your organization added value to its' existing software investment. Microix strives to be at the forefront of technology. From the very first product we released, to our latest, the goal has always been to innovate at every turn.
We are also committed to providing technical support that exceeds your expectations. We know that no matter how good a product might be, it still requires a dedicated team of individuals fully committed to supporting it. From the most rudimentary inquiries to complex problems, our support team is ready to help your organization achieve its' objectives.
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Journalist Richard Rushfield has covered American Idol for four years, since Season 6. In American Idol: The Untold Story (Hyperion, $24.99, on sale Tuesday), he takes readers behind the scenes of America's most popular TV show. Season 10 begins Wednesday on Fox (8 p.m. ET/PT) with new judges Jennifer Lopez and Steven Tyler.
Richard and I recently had a long conversation about his book. A portion of that interview appears in Friday's print edition of USA TODAY. I'll run more of it here, starting with this:
Q: What is the "untold" story of American Idol?
A: So many parts of the show seem like part of the natural order of the universe — a three-judge panel, for instance. When they created it, it was a combination of luck, some smart decisions and a lot of things that could have gone differently and produced a very different show. It's also the story backstage, about producing something that no one expected to be a hit. The show was completely written off when it first aired in America. It was considered a Star Search retread.
When the show came along, it changed the lives of all the people involved, from the contestants to the producers to the stars. It changed their relationships with each other.
Q: What was different about American Idol and Star Search? Why did Idol become what it was, while Star Search, for the most part, remained relegated to the fringes of syndication?
A: Simon Fuller's plan was that you turn the controls over to the audience. That it's not about judges. This was in response what he saw as a music industry that was becoming more concerned with being cool than producing hits and getting further away from its populist roots.
He first conceived of American Idol as an online show that would exist entirely on the Internet, and this was at a time when relatively few people had Internet access. He first conceived it that way, with the idea that this show was entirely put in the hands of the people, that the audience would decide who the winner was.
No one had done that with a show on a big scale before. There had been little bits of voting, but nobody had completely turned a show over to the audience like that before.
Q: What other ways did American Idol change television and popular culture?
A: On the one hand, a singing contest is the most traditional and conservative of memes. One of the very first hits on TV was Ted Mack's Amateur Hour. Singing contests probably go back to the ancient Greeks or something.
What Idol did that was different was bringing this interactive age to television, creating something that the audience controlled that was very much in real time and evolved with the audience control, with the judges' reaction. It opened up and allowed the audiences to feel like they owned the show.
The audience will often be "more Catholic than the pope" in their outrage over alleged infractions of rules. They get more upset than the producers do when it comes out that someone had a recording deal or something like that. Tens of millions of people really feel like American Idol is their show and invest a lot of emotional energy in how it turns out. I think that's something unprecedented, and that's something a lot of shows have tried to replicate.
If you look at competition shows, it seems like half the airwaves are dominated by them now. Idol completely opened up the world to that.
Q: What access did you have to people on the show?
A: I've been working on the book quietly for four years, as I've covered the show. I've gotten to know dozens of the contestants and the producers. As I formally began to write the book, they were very open to me. Most important, Simon Fuller, the creator of the show, told me for the first time the complete story of the creation of the show.
Q: Did you also get to sit down with Simon Cowell?
A: During the course of the four years' previous, I interviewed just about everybody involved for the book. I had never sat down with Simon Cowell.
About three weeks before the book was due, I got a call saying, "Simon Cowell would love to chat with you. When will you be in London?" I said, "I'll be on the next plane."
I raced over there and visited with him. He was very gracious with his time and very open and forthcoming.
Q: You sometimes depict Cowell's motives as inscrutable. What answers did you get about the kind of people he picked for the show in later seasons?
A: A lot of times in Idol history, people have suggested that Simon's motives were more complex than he admits to, more that meets the eye. A lot of people -- and this includes people involved with the show -- believe he has intentionally picked lesser contestants so that he can make fun of them. In the end, there were some people looking towards the divorce [between Cowell and the show] who suggested he had done that to hurt the show. Obviously, he disputes that version, and there's no way of knowing his motives absolutely. But this was a common theme.
What he says to that frankly, is that if he had been doing that, the audiences wouldn't have taken them seriously; if he had been intentionally selecting bad contestants to hurt the show, the audiences would ignore him and go a different way.
There was one incident in Season 7 -- and this was about the only incident in the book where people had diametrically opposed versions of the story -- where Nigel Lythgoe said there was a huge dispute that led to a permanent rift within the Idol production about whether a certain contestant should be brought on the show. Nigel suggested he wanted to bring on that contestant in order to make fun of him.
When I put this to Simon, it was the one point in our interview where he got really upset and strongly, strongly disputed that version. He said he thought this person, Kyle Ensley, was talented, that he would have had a following, that he spoke to the young people. They remember it differently. Both accounts are there, and the readers can decide, and they can view the tape of the original episode, where you see Simon about as angry as you ever saw him on Idol.
It's interesting to watch. He is just fuming. Having now watched every single tape of every single episode, I can tell you that's the angriest he ever was on the air. There was definitely tension there. What it was, readers can decide.
For more of the Richard Rushfield interview, go here:
The Richard Rushfield Interview, Part 2: The 'other side of the Idol world'
The Richard Rushfield Interview, Part 3: 'Hopefully that myth can be put to rest'
I'll post more of my interview with Richard Rushfield throughout the weekend, in which he'll discuss Cowell's departure, the primary myths that surround American Idol (including Season 8's mysterious Arkansas votes), some of the ancillary figures in the Idol world and why they're important, and the changes to the show for this season and through the years.
Nashville-based Brian Mansfield began writing about music for USA TODAY in 1997 and took over Idol Chatter just before the start of Season 8. He co-authored Make Me a Star: Industry Insiders Reveal How to Make It in Music, and while he has never auditioned for American Idol, he did sing Boot Scootin' Boogie at Disney World's American Idol Experience the day the attraction opened (he lost). More about Brian | E-mail Brian


text:
Location: Grain Quality and Structure Research2010 Annual Report
1a. Objectives (from AD-416)
Determine the chemical, physical and sensory attributes in "off" odor sorghum grain for the development of a standard reference sample.
1b. Approach (from AD-416)
GIPSA and ARS are involved in the independent research of measuring grain quality characteristics. The parties agree that meeting the objectives of this project will serve to strengthen and enhance the research programs of both entities by furthering the understanding of improved tools and techniques to determine unacceptable odors in grain sorghum and potentially other grains. It is intention of the parties to this Agreement that the research work shall affirm their mutual interest in cooperative research programs and exchanges and shall be for their mutual benefit and the benefit of the people of the United States. GIPSA request ARS to conduct research on the impact of "off" odor in sorghum on the chemical, physical and sensory quality of sorghum grain. GIPSA agrees to award ARS resources to conduct the above research and provide samples with different degrees of off odors. ARS agrees to study physical and chemical variations between molded and healthy sorghum grain. ARS will provide a sub-award to Kansas State University to complete supplemental work to obtain chemical and sensory traits. The project will be conducted as collaboration between GIPSA-ARS. A major criterion for evaluating sorghum grain quality is odor. Both domestic and international customers are very adept in their ability to detect "off odor" in sorghum grain. If an "off odor" exists a red flag immediately is raised and the quality of the sorghum is suspect. ARS would benefit by collaborating with GIPSA on this project to determine a means to evaluate for "off odor" and the inherent chemical components that comprise the "off odor(s)". The collaboration may lead to the development of a sensory panel for GIPSA that may be used in assessing sorghum grain quality using sensory and chemical analyses.
3. Progress Report
Some volatile compounds present in musty sorghum can be associated with musty odor notes, and can be differentiated in two groups: "dry" and "wet" mustiness, that combine to give an overall musty that is evaluated by grain inspectors. Musty sorghum with "wet" odor notes seemed to have other off-odorant notes as earthy/damp or moldy. The presence of these odor notes may indicate a higher grade of deterioration in the grain, compared with the "dry" musty grain samples. The presence in these samples of some aromatic compounds (such as pyrazines and methoxybenzenes), which were absent in the "musty, dry" sorghum grain samples, corroborated the sensory analysis results. Some references/standards were elaborated using the instrumental aromatic data and the sensory descriptive analysis as the principle potential reference materials for mustiness in grain. These references can allow the USDA inspectors to unify and increase the consistency of the sensory analysis used to grade the sorghum grain. During the next months of the project, new chemicals will be considered (e.g. pyrazines) and used as references. Shelf life study will be continued, and recommendations about references preparation and use can be made. An aspect that needs to be considered for future work is that clean grain samples should be stored in various ways to determine how those storage conditions affect the appearance or disappearance of chemical aromatic compounds over time, and to study the relationship of these compounds with the degradation of the "clean grain odor". Such study would allow recommendations to be made for grain storage.
4. Accomplishments


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Spirit Halloween Costumes
Spirit stores start popping up around September and are so easy to get to. They're practically everywhere in addition to being online and have the most complete collection of costumes I've eve seen. They've definitely solved my costume dilemmas numerous times.
Super Bright American Made Halloween Glow Sticks. Each Glow Stick comes with a lanyard to make in to a necklace.Added 8 Years Ago from Glowstickfactory