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1: It seems that because of the point spending system on stats now that if you were to play a conjurer for a while and spend stats that favor that class you would not be able to switch on the fly to a class like pugilist or lancer which uses str and dex rather than int and piety.
Is there a work around for that? should I spend points so that I am balanced and let my gear focus me or do casters stick to casting classes and melee to melee.
2: I am having trouble picking my guardian and I am wondering what all it effects so I can make a good choice.
3: How stat reliant are the other profs like fisherman and cook and such.
4: How difficult is it to travel from Limsa to Uldah and how important is your starting kingdom.


text:
AFP CO: Obama's Failing Agenda in Aurora
Americans for Prosperity - Colorado
Thursday, October 18, 2012 at 1:45 PM (MDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
Coming to Colorado...
Obama's Failing Agenda Tour
with special guest Monica Crowley!!
Thrusday, October 18
1:45-2:15 PM MT: Rally
Red Lion Hotel
3200 South Parker Road
Aurora, CO
Americans for Prosperity is going coast to coast via bus on the Obama's Failing Agenda Tour to educate Americans about President Obama's failing agenda, and to put grassroots pressure on him.
Join us to rally for economic freedom, limited government and less regulation.
When & Where
Red Lion Hotel
3200 S Parker Rd
Thursday, October 18, 2012 at 1:45 PM (MDT)
Add to my calendar
Americans for Prosperity - Colorado
Americans for Prosperity - Colorado is the largest free-market grassroots organization in the state with over 127,000 Colorado activists holding government accountable as we speak with tens of thousands of citizens, our state legislature and members of the media about critical public policies that promote economic freedom and opportunity for all.

subset: commoncrawl


text:
For decades, only you knew the television programs you watched from your living room. That's starting to change. The FTC recently explored this at its Smart TV workshop. The data generated when you watch television can reveal a lot about you and your household. So, before a company pulls up a chair next to you and starts taking careful notes on everything you watch (and then shares it with its partners), it should ask if that's O.K. with you. VIZIO wasn't doing that, and the FTC stepped in.
Did you get a high school "diploma" from Stratford Career Institute or are you currently enrolled in its high school program? If so, you want to read this. Last February, the FTC sued Stratford Career Institute for misleading advertising of its high school program. Today, the FTC announcedd that Stratford has agreed to return money to people it deceived.
If you decide to buy a water filtration system, there are a lot of choices. If a company says its filtration products are "Built in the USA," that might influence your decision. But what would you think if you learned those filtration products were actually imported from overseas?
Today, the FTC announced a settlement with Volkswagen over 3.0 liter diesel cars the company claimed had low levels of harmful emissions, but did not. The settlement affects more than 77,000 cars made by VW, Audi or Porsche. These cars were not covered by the settlement announced last year, which covered similar false advertising for VW and Audi 2.0 liter diesel cars.
Tax season has just begun, but tax identity thieves already are posting their "gone phishin'" signs: fake emails designed to trick companies into handing over their employees' personal information. To help small businesses avoid the hook, the FTC and the IRS are hosting a free Tax Identity Theft Awareness Week webinar on Wednesday, February 1, at 4 p.m. EST.
Today we're kicking off Tax Identity Theft Awareness Week. Tax identity theft happens when a scammer files a fraudulent tax return using your Social Security number (SSN) and claims your refund. It also happens when someone uses your SSN to earn wages, and sticks you with the tax bill.
Have you heard about a government program that will pay your monthly bills for an up-front payment or processing fee? Here's a short version of the rest of this post: It's a scam. Don't do it.
We've heard that this scam is happening in some African-American church communities: people approach church-goers with this so-called deal. And, because it comes up in church, the scam might seem like it could be legit. But take it from me – and the FTC: there is no federal program that pays your monthly bills in exchange for payment of any kind.
Whether you're gathering with friends for Friday night happy hour or a Super Bowl party to watch the big game, it's important to get home safely. So wouldn't it be great if you could transform your smartphone into a breathalyzer to make sure you were safe to drive? Well, Breathometer, Inc., claimed its breathalyzer devices could do just that, and give you the power to make smart decisions while you're drinking.
When you're on the hunt for income, the last thing you want is to be misled about the earnings and benefits that a business opportunity offers. Today, the FTC announced that Uber agreed to a $20 million settlement of the FTC's charges that the company made false or unsupported claims regarding its drivers' likely income and the benefits of its Vehicle Solutions Program.
Protecting consumers' privacy and personal data has long been a priority at the FTC. Over the years, we've helped millions of identity theft victims recover from that crime. We created the National Do Not Call Registry to limit unwanted telemarketing, and we continue to fight illegal robocalls. And we've brought more than sixty cases against companies that didn't take reasonable steps to protect people's data.
The Federal Trade Commission (FTC) is the nation's consumer protection agency. The FTC works to prevent fraudulent, deceptive and unfair business practices in the marketplace.
Share Our Resources. Here's How >
File a Complaint with the FTC >


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text: Culture 6 April 2011 The real Mona Lisa A team of art historians is preparing to excavate a cemetery in search of the Mona Lisa's model. But Sign up for our weekly email * Print HTML "Wonder is the feeling of a philosopher and philosophy begins in wonder," wrote Plato in one of his dialogues: after all, he argued, Iris, the Greek messenger of heaven, was the child of the sea god Thaumas, who was the concept of wonder personified. But you don't have to be a divine genealogist to appreciate the value of wonder to our understanding of the world around us and our relationship with art -- the vehicle of elucidation that brings dimly felt experience into the light (as Tolstoy once put it). Wonder and enchantment, by their nature, stem from vagueness and imprecision; much of their power lies in their ability to cast our imaginations adrift. Curious, then, that a group of art historians is resorting to CSI-style forensic methods to discover the identity of the model who sat for Leonardo Da Vinci's Mona Lisa. Silvano Vinceti, who raised eyebrows in February with his claim that Da Vinci had based the iconic portrait on the face of Gian Giacomo Caprotti, his young male apprentice, has now embarked on another widely reported project to locate the remains of Lisa Gherardini, the rich merchant's wife long held by convention to have been the sitter. Last year, the same group of scientists announced that it had found the bones of Caravaggio and identified a possible cause of death, centuries after the artist died in mysterious circumstances. The team has also used modern methods to reconstruct the faces of some notable Italians (including Dante) on the basis of their skulls. One obvious question springs to mind: why did they bother? Vinceti was once a television producer and his nous for generating publicity is not in question. Yet his enterprise is more than just a waste of money -- it is a symptom of a culture of cynicism that aims to replace the wonder of art with cold, hard fact. The Mona Lisa, of all paintings, is one that is celebrated for its sense of mystery. For centuries, critics and casual viewers have contemplated the precise meaning of her smile and her story. (As Bob Dylan speculated in the song "Visions of Johanna": "Mona Lisa must have had the highway blues/You can tell by the way she smiles.") The haze of uncertainty that surrounds the work has kept it alive. Anchoring the image to a particular body and identity will neither enrich the experience of looking at it, nor answer the more metaphysical questions that count. In the Prelude, Wordsworth's daydreams transfigure a wet, black rock in the distance into a "burnished shield", glistening in the sun. Not knowing whether his fancies are right or wrong in objective terms allows for imaginative play. In many ways, I'd rather have the shield than the rock. The real-world details of the Mona Lisa's creation would, at most, serve as a mildly diverting piece of supplementary information. Maybe I'm being over-critical of Vinceti's work -- having more information about something, I admit, is better than having less. Yet I reserve a certain scepticism for anyone who feels compelled to make such a song and dance about a relatively insignificant biographical detail, as if solving the mystery of who the model was would, at the same time, solve the grander mystery of the painting's power. Yo Zushi's most recent album, "Notes for Holy Larceny", is out on Pointy Records (£9.99) › Web Only: the best of the blogs Yo Zushi is a contributing writer for the New Statesman. His latest album, It Never Entered My Mind, is out now on Eidola Records and is on Spotify here. Subscribe from just £1 per issue More Related articles How to talk about Harry Potter if you've never read Harry Potter Everyday superheroes - how pop culture can help overcome trauma Upon Remembering Westminster Bridge Subscription offer 12 issues for £12 + FREE book LEARN MORE Close This week's magazine


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UK Dating
Hi Support I was on your site about a month ago and when I cancelled I did not give this site a good review Sorry... more..
Other UK Dating Group Sites
Jeff Goldblum 'dating heiress'Posted on: January 27, 2011 16:58:21
Jurassic Park star Jeff Goldblum appears to still be a hit with the younger ladies after he was spotted kissing his alleged new partner Lydia Hearst-Shaw.
The actor was seen acting affectionately with the 26-year-old heiress and model outside a restaurant in Los Angeles, the Daily Mail reports.
At 58, the Hollywood icon is twice as old as his new girlfriend, but significant age gaps seem to be no unusual thing for the actor.
Goldblum previously embarked on a relationship with Lost star Tania Raymonde, who was just 21 at the time.
However, he seems to be more than happy with his latest love, who is the daughter of Patricia Hearst-Shaw who was famously kidnapped by the Symbionese Liberation Army in 1974.
As well as starring in Steven Spielberg's 1993 hit dinosaur movie, Goldblum also appeared in the film Independence Day and had a cameo role in an episode of Friends.
Are you single? Why not try online dating?
Related USA Dating News
Dazzling engagement ring flashed by Kim KardashianPosted on: October 25, 2013 13:28:01
Kim Kardashian and Kanye West stepped out for their first date as an engaged couple last night October th - and Kim was sporting a huge ring nbsp It was...
(read more)
Lovesick man offers $1,500 for someone to find him a girlfriendPosted on: September 27, 2013 16:11:45
A man from the US has created his own website to try and get a girlfriend - and has offered a pound quot finders fee quot to anyone who helps...
(read more)
Leonardo DiCaprio getting serious with Toni Garrn?Posted on: August 16, 2013 11:33:51
He may be one of the most eligible bachelors on the planet but it seems Leonardo DiCaprio has only got eyes for one woman at the moment The Great Gatsby...
(read more)
Ashley Tisdale and Christopher French engagedPosted on: August 13, 2013 13:35:48
Hollywood starlet Ashley Tisdale has confirmed she is engaged to her rocker boyfriend Christopher French The blonde beauty who rose to fame in the High School Musical franchise confirmed via...
(read more)
News Index
Online Dating (1340)
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Mobile Dating (81)
Gay Dating (20)
Christian Dating (12)
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text:
Pay-Per-View Cleanup
To meet Pro Wrestling Wiki's quality standards, this PPV article or section may require cleanup. Please help by improving the article.
IPW Genesis 2008 was a Professional wrestling event produced by IPW which took place on at the Lynfield YMCA in Lynfield, Auckland.
- (c) refers to the champion(s) heading into the match.
- Evie defeated Britenay (w/ "Handsome" Danny Jacobs & Paul Starr)
- Pirates and Ninjas Are Totally Awesome defeated Saint Chaos (w/ special guest referee Ricky DeVinal)
- "Dangerous" Vinnie Diamond (c) defeated Link van Haggard to retain the South Pacific Championship
- Jon E. King defeated IPW Commissioner Daddy Kool (w/ Mischa Vladimir Ivanoff)
- Alfred Valentine w/ Flagboy vs. "The One" Vinny Dunn ended in a Time Limit Draw
- "The Samoan Silverback" Alexander defeated "The Professional" Joseph Kinkade (w/ Gary O. Davis & Gorilla)
- Xtreme Superstars defeated "The Deal" Dal Knox & The Fire Ant
External links
- [ Official link]


text:
Braden Castle Association
Bradenton, FL 34208
About Braden Castle Association
In 1924 a committee was formed and the property, now known as "Braden Castle Park" was purchased. The property today consists of 200 lots, of which 197 are built on with small houses, a trailer park that has 94 mobile home sites, and ...
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text:
|Publication number
|Publication type
|Application number
|Publication date
|Feb 23, 2010
|Filing date
|Sep 14, 2004
|Priority date
|Sep 15, 2003
|Fee status
|Also published as
|Publication number
|James Guest, Carlos Casas
|Original Assignee
|James Guest
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (8), Non-Patent Citations (3), Referenced by (3), Classifications (11), Legal Events (8)
|External Links: USPTO, USPTO Assignment, Espacenet
The present application claims the benefit of U.S. provisional application no. 60/503,134 filed Sep. 15, 2003. The provisional application is hereby incorporated by reference.
The invention relates generally to a method and system for cellular transplantation, and in particular, to percutaneous endoscopic cellular transplantation into the spinal cord to create intramedullary cellular trails.
Stereotaxic injection into the brain is facilitated by secure cranial immobilization in a stereotaxic frame. This has made it possible to accurately place transplants in the brain. However, stereotaxic injection into the spinal cord is more difficult due to the small size of the spinal cord, its constant motion in several planes relative to the vertebral spine, and its exquisite vulnerability to injury. For these reasons current spinal cord injection techniques rely upon direct visualization of the spinal cord during an open surgical procedure and immobilization of the spine in clamps during cellular injections. Since this type of surgical exposure is usually necessary to create an experimental spinal cord injury, various types of transplant techniques are possible using the same exposure.
Many spinal injuries require decompression and vertebral column stabilization surgery soon after hospitalization. This surgical opportunity may not be an optimal time for cellular injection to facilitate repair. Once this initial surgery has occurred, it is undesirable to disturb the progress of wound healing and bone fusion. Therefore, it is important to develop strategies of cell transplantation into the spinal cord compatible with minimal disturbance of the wound healing process. Cellular transplantation into the injured spinal cord has been used to study injury and repair responses in experimental models. Some clinical trials of cellular transplantation (e.g. activated macrophages, ensheathing glia) into the injured human spinal cord have been initiated using conventional open surgical techniques. Cell suspensions can be transplanted to create trails or columns of cells to partially reconstruct axonal pathways. In the spinal cord this requires either several closely spaced separate injections or a technique to enter the cord at a shallow angle. Currently, almost all injections into the spinal cord result in a tract defined by the needle path which is generally perpendicular to the longitudinal axis of the spinal cord.
There exists a need for a minimally invasive technique and system for cellular delivery in the spinal cord. Accordingly, the present invention provides a minimal access endoscope-assisted technique via a lumbar puncture approach without the need to create a large surgical incision. Cellular transplantation is performed under endoscopic visualization based on percutaneous access, remote from the injury site. Delivery of cells or cell suspension provides a trail of cells in the spinal cord which may span from the site of axonal injury to a useful target neuronal population. Regeneration of the damaged axons to the target neuron occurs via the transplanted supportive cells. The trail of cells is defined by the needle path which is generally parallel to the longitudinal axis of the spinal cord.
Spinal cord injections may be performed by introducing an endoscope percutaneously at an interlaminar space, for example a lumbosacral interspace. Because the epidural and subarachnoid spaces can be distended with fluid injection, endoscopic visualization of structures in these spaces can be achieved. The endoscope may be used with a syringe to inject a cellular suspension using a small catheter and endoscopically directed needle placed through the working channel of the endoscope. The system and method results in a columnar trail of transplant cells.
This strategy for intraspinal injection may confer important clinical advantages over other techniques. For example, using an endoscopic procedure instead of a CT-guided percutaneous technique, the spinal cord surface blood vessels can be visualized and avoided to prevent bleeding. Also, orientation to the midline is improved, and the depth of the needle insertion can be directly determined. As a further advantage, insertion of the endoscope at a non-injured site permits a cautious traversal of the endoscope from a normal region into the injury region of the spinal cord, thereby improving visual orientation. Also, the present invention eliminates the need for a laminectomy to access the spinal cord, facilitates flexibility of timing between surgical decompression and transplantation operations, reduces interference with healing of previous surgical incisions and fusion beds, facilitates multiple episodes of transplantation at different sites, reduces surgical duration, and may be performed under local anesthesia.
In accordance with one aspect of the present invention, there is provided a method for treating an injury of a spinal cord of a patient. The method includes implanting a therapeutic substance in the spinal cord under indirect visualization, such as an endoscope. The endoscope may be inserted through a skin puncture site remote from the injury of the spinal cord and through an interlaminar space of the patient, such as a lumbosacral interspace.
The method may further include inserting an injection member through the skin puncture site and into the spinal cord and injecting the therapeutic substance through an aperture of the injection member. The injection member may be at least partially withdrawn from the spinal cord while injecting the therapeutic substance to thereby form a trail of therapeutic substance. The injection member may be slideably disposed in a working channel of the endoscope and may include a needle connected to a catheter. The distal portion of the needle may be inserted into the spinal cord.
The method may also include reducing or damping motion of the needle in the spinal cord relative to the endoscope by providing a flexible portion of the catheter between the needle and a distal end of the endoscope. The therapeutic substance injected into the spinal cord may be cells, a cell suspension, or a biological matrix containing a therapeutic substance(s). The method may further include injecting distention fluid in the interlaminar space.
In an exemplary embodiment, the method may include the insertion of an implant into the patient. Use of percutaneous endoscopy may place syringopleural or cystoperitoneal cerebrospinal fluid (CSF) shunts within the spinal cord. Endoscopically-assisted shunting of intramedullary syringes, an arachnoid cyst, and/or a perineurial cyst may be performed.
In accordance with a similar aspect of the present invention, the injection member may include a catheter connected to a needle with a side-hole aperture. A flexible hollow guide wire may be positioned through the catheter and side-hole aperture of the needle and into the spinal cord. The therapeutic substance may be injected from the hollow guide wire while the guide wire is being withdrawn from the spinal cord to thereby form a trail of therapeutic substance in the spinal cord.
In accordance with another aspect of the present invention, a device is provided for treating an injury of the spinal cord of a patient. The device includes a visualization means for visualizing the spinal cord through a skin puncture located remote from the injury and an injection means for injecting a therapeutic substance into the spinal cord. The visualization means may be provided by an endoscope. The injection means may be withdrawn while injecting the therapeutic substance to thereby form a trail of therapeutic substance in the spinal cord. The device may also include means for fluid distention of an interlaminar space of the patient. Fluid distention facilitates endoscopic visualization because the optical density of the fluid permits illumination and visualization.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
A more complete understanding of the present invention, and the attendant advantages and features thereof, will be more readily understood by reference to the following detailed description when considered in conjunction with the accompanying drawings wherein:
The present invention provides a method and apparatus for making a percutaneous cellular injection into the spinal cord under endoscope visualization. The method includes percutaneously introducing a flexible endoscope into the subarachnoid space of the spinal cord, introducing a flexible catheter with an attached needle into a working channel or lumen of the endoscope, penetrating the spinal cord with the needle under direct visualization of the endoscope, and injecting cells into the spinal cord while the needle is withdrawn. The steps of penetrating the spinal cord and injecting cells into the spinal cord result in the implantation of a trail of cells within the spinal cord.
The system of the present invention provides for minimally invasive transplantation of cells within the spinal cord. A visualization means provides percutaneous viewing of the spinal cord (and/or interlaminar space, lumbosacral interspace, arachnoid, subarachnoid, pia mater). An injection means provides for the injection of cells into the spinal cord. The injection means may be configured for insertion through a working channel of the visualization means. The system provides a trail of implanted cells within the spinal cord.
Referring now to
Another syringe 54 may be placed in fluid communication with the working channel of the endoscone 34. Distention fluid may be injected into the subarachnoid space 50 using the syringe 54.
In addition, the proximal end of the catheter may include indicia such as 1 mm marks to accurately determine how deep the needle has penetrated into the spinal cord. Also, a PE-10 polyethylene (or other suitable polymer) catheter may be used to hold the injection needle which may be a 30 G cut-down needle tip extending approximately 5 mm from the end of the injection catheter and set about 3 mm into the catheter. The PE-10 catheter may have an inner diameter of about 0.28 mm and a length of approximately 58 cm. As such, a volume of 35.7 μl of cell suspension may be pre-loaded into the catheter. The catheter may be back-filled with a cell suspension so that a continuous fluid column exists in the catheter. The continuous fluid within the catheter and syringe system provides uniform injection pressure thereby facilitating uniform cell dispersion.
The endoscope may be rotated after positioned in the subarachnoid space to optimize the relative positions of the optical and illumination fiber bundle, the working channel, and the dorsal spinal cord to perform the injection. Distention of the subarachnoid space with isotonic fluid may be useful prior to the injection since the endoscopic visualization is enhanced in a fluid filled space. The distention may be delivered by attaching tubing in fluid communication with the working channel of the endoscope so that fluid can be injected via the working channel beside the injection catheter. The fluid infusion pressure may be approximately 30-35 cm H2O and about 100 cc of fluid may be delivered during the procedure.
It is further contemplated that different injection parameters such as volume and injection rate may be utilized to minimize spilling the cell solution into the adjacent subarachnoid space. Also, distension fluid infusion may be stopped just before the cell injection is started to reduce the chance that the flow of fluid would cause cells to disperse from the injection site to other levels of the neuroaxis. Furthermore, the endoscope, catheter, and/or needle may be inserted through the patient's skin at a location distant from the cellular injection site. As an example, for a L1 level injury of the spinal cord conus medullaris, the endoscope is introduced at L5/S1 and then directed several centimeters rostral to the level just distal to the cord injury where the injection could be made provided the subarachnoid space was open. This increases the safety of the procedure by allowing the operator to become oriented to normal anatomic structures prior to entering the subarachnoid space surrounding the areas of spinal cord injury.
Representing another exemplary embodiment of the present invention,
Referring to
Referring to
It is further contemplated that the catheter and needle may be positioned independent of the working channel of the endoscope. In
Clinical Trial
This study employed five cadaveric female pigs weighing approximately 30 kg.
NIH 3T3 fibroblasts were grown in DMEM with 10% fetal bovine serum in a tissue culture flask at 5% CO2 at 37� C. They were labeled with a fluorescent nuclear dye, Hoescht 33342 (Molecular Probes) at a concentration of 1:100. The cells were released from dishes using trypsin and triturated into a suspension. The cell suspension was then centrifuged at 1500 rpm for 5 minutes, the supernatant removed, and L-15 (Gibco) was added to achieve a final concentration of 105 cells per μl. The cells were maintained on ice prior to injection.
The lumbosacral anatomy of the adult pig was studied to determine optimal entry points for percutaneous access to the spinal canal. The effectiveness of two flexible endoscopes was compared for this cell injection technique. The MYELOTEC fiberscope (Model 3000E) is designed for lumbar epidural procedures, and offers the advantage of small size (0.9 mm) but its tip is not independently steerable. The scope is deployed by insertion into a disposable 2.7 mm diameter catheter module (Model 4001) with dual Leur-lockable ports and channels. One channel is used to introduce the fiberscope, and the other can be used for irrigation or, for the present invention, insertion of the injection catheter. These ports provide some resistance to fluid back flow, and the introducer also has a valve to also resist fluid back flow. This is advantageous to reduce CSF loss and to maintain fluid distention of the CSF space. The device is "steered" by rotating a disc on the catheter module.
The other fiberscope that was studied was the CODMAN 83340 Neuroguide IOVS. It is a 38 cm long steerable scope of 3.2 mm diameter with a 3 FR working channel. It has a tip articulation of 100-160 degrees, the depth of field is 5-50 mm, and the field of view is 64 degrees in water. Both scopes were deployed in all five animals. For this trial, however, the CODMAN 83340 scope was used for the cellular injections. The endoscopes were coupled to a high resolution color CCD camera (Endoview, Urohealth Systems Inc., Costa Mesa, Calif.). One video output signal was connected to a monitor, and the second output signal was used to acquire digital video using a Sony DCR-PC100 digital video camera.
To access the lumbar subarachnoid space with the MYELOTEC fiberscope, a 14 G spinal needle (2.1 mm OD) was directed between L5 and L6, 7 cm rostral to the sacral depression until there was loss of resistance in a glass syringe (epidural space). Next, the dura was penetrated and CSF returned and the depth in mm from the skin to the dural opening was measured. A flexible guidewire was placed through the needle into the subarachnoid space (SAS). The spinal needle was removed and the 7F introducer sheath (2.33 mm OD) was advanced over the guidewire. The guidewire was removed and the catheter module introduced. Next, the 0.9 mm (OD) fiberscope was advanced through the catheter module until emergence and visualization of nerve roots was detected. Pulsatile injection of saline through the second channel improved image quality and made it easier to navigate the catheter module and endoscope.
Placement of the CODMAN scope was different than the MYELOTEC fiberscope because of its greater size. A 12.5F peel-away introducer was fed over a large (10 G, OD=3.4 mm) needle placed into the SAS at L5/6. The needle was then removed and the endoscope introduced into the SAS through the sheath. A large syringe (30 cc) was attached via a three-way stopcock to the working channel port and small pulses of saline were injected. Both endoscopes were navigated adjacent to the dorsal or dorsolateral spinal cord surface. Because the CODMAN introducer does not have a valve to prevent backflow, more fluid was used to distend the subarachnoid space since there was a constant loss of fluid through the small gap between the scope and the introducer.
All cellular injections described hereinafter were delivered using the CODMAN endoscope. Injection was performed in a dorsal midline area at the L2 vertebral level. Thirty-six μl of the cell suspension was loaded into a polyethylene tube [0.61 mm OD, 0.28 mm ID] 58 cm in length (Intramedic polyethylene tubing PE-10) with a 30 G needle (8 mm total length, OD 0.3 mm) attached at its distal end. Fluid infusion was stopped, and the catheter was slowly inserted into the working channel and advanced distally until the needle tip could be visualized to exit the endoscope. It was then necessary to rotate the endoscope to optimize the relative positions of the optical fiber bundle, the needle, and the dorsal spinal cord. With the scope facing the dorsal spinal cord surface at a 45 degree angle, the needle was advanced until it contacted the pial surface.
Following pial perforation, the needle was advanced 4 mm along a rostro-ventral trajectory by gently pushing the catheter into the endoscope. At this point irrigation was terminated with the subarachnoid space moderately distended at a pressure of 30-35 cm H2O. One μl of cell suspension was injected, and another 4 ul was injected while the needle was slowly withdrawn from the cord over about 5 minutes. Then, the needle was left in position for a further two minutes before being removed from the spinal cord parenchyma. No further pulses of saline irrigation were delivered in order to minimize the possibility of flushing the cells along the neural axis. The endoscope was removed and 4% paraformaldehyde was slowly infused into the subarachnoid space around the injection site. A lumbar laminectomy was then performed to remove the injected region of the spinal cord for histological evaluation.
In preparation for histological assessment, the specimens were post-fixed in 4 percent paraformaldehyde for two weeks. The cord was embedded in gelatin and cut into 55 μm sagittal sections using a sliding freezing microtome. The sections were visualized using a Zeiss Axiophot fluorescent microscope to identify the fluorescent-labeled injected cells. Digital images were acquired at 10� using METAMORPH software. Image montages were constructed in ADOBE PHOTOSHOP 5.0 to show the full length of the cellular trail within the spinal parenchyma and to assess for spillage at the adjacent spinal cord margins.
A useful anatomic landmark in the pig is a palpable sacral depression (SD) from which the L5/6 interspace was located 7 cm rostrally. The vertical distance from the skin to the dorsal surface of the cord at L5/6 was 6-7 cm. The interlaminar spaces (or lumbosacral interspaces) at L5-6 and L6-S1 at the midline were 4.0 and 2.5 mm respectively. The porcine conus medullaris terminates within the sacrum 3 cm rostral to the SD, thus there is no true lumbar cistern as in the human.
Video image quality was similar using either the MYELOTEC or CODMAN endoscopes. Blood vessels, nerve roots, and denticulate ligaments could be visualized with either endoscope. Adequate visualization of anatomic details during scope navigation required pulsatile irrigation of the subarachnoid space with fluid. Although the size of the CODMAN scope was greater, the ability to control the direction of the injection needle and catheter was better than with the disposable MYELOTEC catheter module. This appeared to be due to stiffness within the scope tip and the fact that the CODMAN scope was only actively directable at the tip. The steering motion of the MYELOTEC scope was more whip-like and it tended to flip from the midline to the lateral spinal canal during attempted injections. For this reason, the CODMAN endoscope was utilized for definitive injections.
A useful feature of this technique is that while the tubing and at least part of the needle are within the scope, the needle behaves as a rigid extension of the scope. This allows for the force necessary to penetrate the pial surface. When a loop of tubing is fed out between the tip of the scope and the needle after insertion into the cord, small motions of the scope and spinal cord are damped with respect to the needle. This has the effect of reducing injection-associated spinal cord trauma by allowing the needle to move with the spinal cord during physiologic motion.
Trial Results
During histological analysis, a trail of Hoechst-labeled cells was identified in each specimen under a fluorescent microscope. Some trails were homogenously distributed whereas others consisted of interspersed clumps of labeled cells. Some cell spillage was evident on the adjacent pia in 4/5 specimens; however, cells were not seen more than 3 mm from the injection site, and neither traumatic distention of the central canal nor cavitation as a result of the injection was observed.
The development of small flexible fiberoptic scopes and high resolution CCD cameras has lead to increased interest in the use of endoscopes for spinal canal applications. Multiple injections into the spinal cord over an extended time period may be necessary for effective regeneration. The technique described herein could also be used to deliver non-cellular biomaterials into the subarachnoid space or spinal cord. Alternatively, other therapeutic effects, such as pain modulation via chromaffin cells transplanted into the subarachnoid space might be achieved with this technique.
Due to camera and display interface associated degradation, an alternative consideration is to use rigid endoscopes with their superior rod lens optics. A technique based on a rigid endoscope would sacrifice some of the advantages of the flexible scope such as entry into the subarachnoid space at a point several segments distal to the injection site and the flat injection trajectory possible by having the flexible scope nearly parallel to the spinal cord. The incorporation of backflow resistance valves into the MYELOTEC introducer and catheter module means that less fluid volume needs to be delivered to distend the subarachnoid space than with the CODMAN introducer where some constant backflow occurs. However, the advantage of not incorporating a valve is that CSF pressures are less likely to be escalated using the CODMAN introducer and endoscope. Using a porcine model of subdural fluid infusion, it has been shown that spinal cord ischemia occurs at induced CSF pressures of approximately 65 mm Hg.
While dural puncture is simple with a spinal needle, getting flexible introducers to enter the porcine dural sheath over a guidewire requires more effort. Therefore, a large spinal needle was used to improve the ability to pass the CODMAN guide sheath through the dura. In clinical application of the 7F MYELOTEC introducer for intradural procedures, there have not been CSF leaks.
The concept of creating cellular trails to guide regenerating axons assumes that cellular migration following transplantation is minimal. Random cellular migration could lead to poorly organized or misdirected axonal growth. In a previous study trails of Schwann cells were not dispersed 6 weeks after injection. Axons grew along the trails in a fasciculated pattern similar to that seen in peripheral nerve grafts. The injection technique utilized in this trial appeared to cause little injury to the spinal cord.
Percutaneous endoscopic cellular injection may be useful for cellular transplantation, may reduce surgical and anesthetic time, be compatible with local anesthesia, eliminate the need to disrupt spinal instrumentation and bone grafts, and allow greater flexibility in the respective timing of spinal fixation and cellular transplantation following spinal cord injury. Using the system and method according to the present invention, intraspinal cellular injection using a flexible endoscope from a percutaneous access is achievable.
All references cited herein are expressly incorporated by reference in their entirety.
It will be appreciated by persons skilled in the art that the present invention is not limited to what has been particularly shown and described herein above. In addition, unless mention was made above to the contrary, it should be noted that all of the accompanying drawings are not to scale. Accordingly, a variety of modifications and variations are possible in light of the above teachings without departing from the scope and spirit of the invention. For example, the invention has been described with reference to a spinal cord procedure. It should be appreciated that the present invention can be applied to a number of situations in which percutaneous introduction of cells or other biomaterial is desired.
|Cited Patent
|Filing date
|Publication date
|May 16, 1988
|Feb 27, 1990
|Janese Woodrow W
|Apparatus for the exchange of cerebrospinal fluid and a method of treating brain and spinal cord injuries
|Apr 24, 1998
|Feb 22, 2000
|University Of Massachusetts Medical Center
|Guided development and support of hydrogel-cell compositions
|Oct 14, 1998
|May 15, 2001
|Daum Gmbh
|Contrast agent for ultrasonic imaging
|Jul 13, 1999
|Oct 22, 2002
|Catheter Imaging Systems, Inc.
|System for enhancing visibility in the epidural space
|Feb 22, 2002
|Jul 18, 2002
|Maria Palasis
|Lateral needle-less injection apparatus and method
|Apr 18, 2002
|Nov 21, 2002
|Cragg Andrew H.
|Method and apparatus for spinal augmentation
|Jul 13, 2001
|Jan 16, 2003
|Purdy Phillip D.
|Methods and apparatuses for navigating the subaracnhnoid space
|Jan 14, 2003
|Jul 15, 2004
|Jahns Scott E.
|Devices and methods for interstitial injection of biologic agents into tissue
|"Schwann cell genetically modified to secrete human BDNF promote axonal regowth across transected adult rat spinal cord". Phililppe Menei et al., European Journal of Neuroscience, vol. 10, pp. 607-621, 1998.
|Casas, CE, EJ Owen, Oliveira M, Guest JD: Endoscopic Transplantation into the conus medullaris. Soc. Neurosci Abstr. 2002 852.8. Nov. 2002.
|Cellular transplantation into the spinal cord using an endoscopic technique. C Casas, E Owen, J Guest. Presented at the AANS/CNS Section on Disorders of the Spine and Peripheral Nerves, Orlando, FL, Feb. 2002.
|Citing Patent
|Filing date
|Publication date
|Sep 8, 2008
|Oct 8, 2013
|Bayer Pharma AG
|Connector system having a compressible sealing element and a flared fluid path element
|Sep 8, 2008
|Mar 11, 2010
|Medrad, Inc.
|Connector system having a compressible sealing element and a flared fluid path element to reduce fluid flow restrictions
|Aug 29, 2012
|Mar 14, 2013
|The Children's Hospital Of Philadelphia
|Device and method for accessing small lumen
|U.S. Classification
|604/506, 604/500
|International Classification
|Cooperative Classification
|European Classification
|Dec 21, 2004
Owner name: GUEST, DR. JAMES, FLORIDA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:CASAS, MR. CARLOS;REEL/FRAME:015476/0970
Effective date: 20041203
Owner name: GUEST, DR. JAMES,FLORIDA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:CASAS, MR. CARLOS;REEL/FRAME:015476/0970
Effective date: 20041203
|Oct 4, 2013
|Maintenance fee reminder mailed
|Feb 23, 2014
|Lapse for failure to pay maintenance fees
|Feb 23, 2014
|Reinstatement after maintenance fee payment confirmed
|Apr 15, 2014
|Expired due to failure to pay maintenance fee
Effective date: 20140223
|Nov 2, 2015
|Patent reinstated due to the acceptance of a late maintenance fee
Effective date: 20151105
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Owner name: INVIVO THERAPEUTICS HOLDINGS CORP., MASSACHUSETTS
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Browse our listing of great programs and events for all ages. Choose Select a Calendar to view events by category or age group. Select the Community Events calendar to view non-library events held in our meeting rooms. Select Notify Me to sign up for email or text message notifications about library events.
We conclude our 40th anniversary season with long time favorites The Nields! Nerissa & Katryna Nields continue to tour the country playing for all ages. The Nields's family shows are a blend of Pete Seeger-esque group song leading and Dan Zanes-like reconstruction of old classics, plus their own inimitable style of high energy, whimsy and filigreed harmony singing.


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Product Information
Important Sizing Note: The following measurements are the dimensions of the actual product, not of the pet they fit. After measuring your pet, make sure all measurements fit within the following dimensions (with a little room to spare in the chest and neck).Small
This is a great comfort harness. I put it on my dog as soon as it arrived. You can tell she's more comfortable with no irritation under her front legs! Thanks!!


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Even though I'm in Taiwan, I find myself often bored. I have yet to transfer money over so I can't travel too much. But my race stuff came today! I'm so excited ^^
I haven't participated in a run since Sept 2012, so I'm very nervous, but also so very excited!
I also made the Captain a box so I can send him some souvenirs.
I was also a bad girl and bought popteen, even though I can't read that much of it. (read any of it)
It makes me want to sew s badly! I also went to the Chaing Kai-Shek memorial, where we saw a protest against illegal lobbying, it was really interested, I felt lucky to be able to see it.
I've been knitting and watching dramas lately too.
I'll let you know how the race goes! I hope I finish it in under 2 hours.


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Whether fashionable everyday modern wear or special-occasion Renaissance Faire garb, many leather garments feature both functional and decorative laced closures. Laces lend boots and shoes, jackets and coats, bodices, corsets, trousers and gloves not only practical solutions, but power and style. However, threading laces through plain leather holes can leave them looking unprofessional or sloppy and risks damaging the leather over time. For a clean, finished look, adorn leather lacing holes with sturdy metal grommets.
Things You'll Need
- Grommets
- Washers
- White chalk
- Leather punch
- Rubber mallet
- Grommet punch kit
Measure the size of the grommets and determine the desired distances between them. Mark the grommet placement on the leather with white chalk.
Center a leather punch over the markings to make lacing holes. Hit the top of the punch with a rubber mallet until it pierces the leather.
Slide a grommet end through a hole in the front side of the leather.
Turn the leather over and slide a washer onto the protruding grommet end.
Place the leather, front side down, onto the anvil in the grommet punch kit. Center the grommet's face side on the anvil.
Place the grommet punch through the center of the grommet. Hit the punch sharply with the mallet until the washer folds, securing the grommet. Repeat the process on all lacing holes.
Tips & Warnings
- Set the anvil on a block of wood to cushion the floor, table or workspace.
- Select grommets with teeth only when working with heavy leather, as these may loosen with use in lightweight leather.
- Select the same size leather punch as the grommet size.
- In addition to hammer punches, setting machines and plier punches are optional. Pliers, however, do not give as clean a result as the others.
Related Searches
- Making Leather Handbags and Other Stylish Accessories; Ellen Goldsten-Lynch, Sarah Mullins and Nicole Malone
- Sew and Repair Your Outdoor Gear; Louise Lindgren Sumner
- Photo Credit Jupiterimages/Photos.com/Getty Images


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Ebook Misteri Dunia Jar
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LINCOLN, Neb. – After a strong exhibition performance on Monday night, Nebraska basketball will kickoff their season on Saturday against Mississippi Valley State.
Coming off an unexpected 13-18 seasons, Tim Miles enters his fourth year as the head coach of the Huskers. Just two-years ago, Miles was named the National Coach of the Year after getting Nebraska into the NCAA Tournament.
"It's hard to believe it's already here again," said Miles. "I heard the PA guy say, 'in his fourth year.' I thought to myself, I just walked in the door. What are you talking about?"
Nebraska will be one of the youngest teams in the Big Ten in 2015-16. The Huskers could play up to five true freshmen in the rotation this season. Guard Glynn Watson, guard Evelyn Bakari, forward Ed Morrow, forward Jack McVeigh and forward Michael Jacobson all played against Northern State on Monday.
"I thought they were okay. They have a long way to go," said Miles. "It was good, because Northern gave us a different look than Wyoming (scrimmage opponent) did, which is a different look than in practice, which is probably a different look than we will get against Mississippi Valley or Nova. They are going to have to keep making adjustments in their game. It's not like you are just running up and down playing against similar talent. You are running against older guys who are game planning for you."
With a younger group of players, seniors Shavon Shields and Benny Parker have had their hands full trying to get the squad ready to compete every night.
"There connection with these guys is everything," said Miles. "The young guys are impressionable for a time…the upperclassmen will tell those guys, 'we need you. We are depending on you. We want you to be good. Listen to coach.' There is a real connection that way of teaching and learning."
Earlier in the week, Miles wasn't ready to commit to how deep his rotation will be in the early part of the season.
"There are times I think we have four and there are times I think we have nine," said Miles. "Somewhere in between. I hope we can get five for sure."
Big Red Report Projected starting lineup:
G.) Benny Parker – The high-energy defensive presence has played in 96 games at Nebraska.
G.) Tai Webster – After struggling his first two years, an aggressive Webster could be an x-factor this season.
F.) Andrew White – A Kansas transfer, the junior scored 18-points against Northern State on Monday night.
F.) Shavon Shields – The Huskers returning leading scorer averaged 15.4 and 6.0 rebounds last season.
F.) Jacob Hammond – Will be asked to contribute big minutes this season after only playing in 11 games last year as a true freshman.


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Located on the seventh floor, with views over the city, the Mississauga Executive Centre is in Tower 2 of a landmark cluster of four 16-story office buildings at the centre of Mississauga's commercial district. The centre, in the western part of the Greater Toronto Area, is on Hurontario Street (Hwy. 10) just opposite Square One, Ontario's largest shopping mall, with its selection of restaurants and facilities. Secure underground parking is available for tenants.
Mississauga is Canada's sixth-most populous municipality, almost doubling in population in each of the last two decades. More than one in ten of Fortune 500 base their Global or Canadian Head Offices in Mississauga. Some of the strongest industries are pharmaceuticals, Banking and Financials, electronics and computers, transportation parts and equipment industries.
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Cisco 1750 & 1751 flash memory maximum upgrade capacity?
Hi guys,
Need your help on this. Can I know, what is the maximum flash memory capacity can I upgrade on Cisco 1750 & 1751. I need to use minimum 32M of flash memory to upgrade my routers to IOS version 12.3 and up.
And also can I upgrade the memory to 64M? I think there is no problem with the memory upgrade, only concern about the flash upgrade. Especially for 1750 routers.


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A Place to Put Your Cash
This Horizon Three Wallet from Makr Carry Goods is truly a man's man wallet that has luxury and quality written all over it. It is a three pocket card holder and has one exterior pocket. It is hand sewn, and the edges have even been painted and polished to perfection. This wallet is constructed of Bark HF Horween Chromexcel leather, so you know it is guaranteed to be durable.
At $90, we think you should do it for your money.
Incoming Search Terms:
- makr horizon three wallet


text:
Find out how much this building will cost in your area today
with our online estimating tool, the DCD Archives.
to create a conceptual estimate instantly on this building
and hundreds of other RELIGIOUS projects.
DCD Subscribers
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Project Architect/Mechanical & Electrical
SOA Architects dba Stuart Owsley & Associates, PA
902 South Taylor, Pittsburg, KS 66762
General Description
Location: Branson, Missouri
Date Bid: Jan 2008
Construction Period: Apr 2008 to Apr 2010 Total Square Feet:
19,200 Site: 1.5 acres. Number of Buildings: Three; Chapel seats
Building Size: First floor, 19,200; total, 19,200 square feet.
Building Height: First floor, 50' maximum; tower, 80'; building
main floor, 10 to 18'; cross/geodesic sphere, 20'; total height of
tower, 100'.
Basic Construction Type: New/V-B.
Foundation: Cast-in-place, reinforced concrete, slab-on-grade,
retaining walls. Exterior Walls: Native stone veneer. Roof:
Metal. Floors: Concrete, stone. Interior Walls: Wood stud
Construction Team
Administrative Architect (Construction Phase): Treat Architects,
P.C. - 415 Green Briar Drive, Branson, MO 65616
Visionary/Consultant: O. Gene Bicknell, Bicknell Family Holding
Company, LLC - 4200 West 115th Street, #100, Leawood, KS 66211
Project Manager: Michael Rankin, Rankin Consulting - firstname.lastname@example.com
General Contractor: Larry Snyder & Company - 4820 North Towne
Centre Drive, Ozark, MO 65721
The completed shrine, inspired from an idea that came to entrepreneur
Gene Bicknell in a vision five years prior to its dedication on April
11, 2010, is now the "Shrine of the Holy Spirit" in Branson, Missouri.
Bicknell commissioned SOA Architects to give his vision form, shape and
detail with conceptual and construction drawings and specifications.
Stuart Owsley, project architect needed to also understand and share
this vision from a sculptor's perspective through sketches, dialogue and
reflection with and from Bicknell.
The Shrine project features a one hundred foot high triangular shaped
tower representing the Trinity, which is topped off with a cross and
geodesic sphere representing the world. At each side of the tower, fifty
foot high splayed and scalloped top wing walls constructed with
bluestone veneer panels and representing the shoulders of God, continue
down to encompass the Chapel building with side walls representing the
arms of God, which welcomes visitors into his presence.
Inspirational scripture verses are engraved on large bluestone panels
recessed into the "shoulder and arm" walls and viewed as visitors walk
around outside the Chapel walls. While the tower and walls are
dominating elements of the shrine, they highlight the chapel building
and there is no doubt as to its importance. This small hexagon shaped
structure is constructed with native limestone rubble veneer, six sided
pyramid shaped roof, stained glass arched windows, triangular shaped
bluestone floor tiles and pew seating occupancy of eighty for
comfortable intimate worship, prayer and quiet reflection.
The Shrine project includes four multi-tiered hexagon shaped
interlocking courtyard decks covered with bluestone tiles and surrounded
by descending bluestone walls that encompass the hexagon shaped gift
shop/restroom building and hexagon shaped gazebo structure for outdoor
reflection and relaxation – three structures representing the Trinity.
The lowest center main courtyard deck includes a hexagon shaped
fountain/pool centered between the main steps going up to the Chapel
deck, stone tablet monument engraved with Ten Commandments, flower bed
planters and comfortable bench seating. The entire structure is recessed
twenty feet into solid rock at the tower base and positioned on a
sloping site directly in front of The Mansion Theatre in Branson. Four
hundred tons of the bluestone material was quarried, cut and furnished
by American Bluestone, LLC from upstate New York.
Owsley truly believes he was also inspired by the Holy Spirit and
Bicknell's vision and will give credit to Bicknell and the Holy Spirit
for his once in a lifetime opportunity and contribution. The local firm
of Treat Architects, Inc. handled the architect's administrative duties
for 20 months of the construction phase. The Shrine is dedicated to the
Holy Spirit, but also testifies to the perseverance and faithfulness of
a man in his vision and the guidance of the Holy Spirit. "It was not an
easy journey at times, but it was truly driven by the Holy Spirit."- O.
Gene Bicknell
Product Information
Structure Wing Walls: American Bluestone LLC
Metal Roofing: Architectural Metal Roof System
Lighting: Shaper, Invue, LSI Composite Walls: Alcotex
Entrances & Storefronts: Special-Lite, Inc.
©2015 Copyright DC&D Technologies, Inc. All rights reserved. | DCD
Construction Magazine | Email:


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Guaranteeing Sales Success With 3 Key Outbound Sales Techniques
Similar to the bright and colourful display of a bird in search of a mate, content marketing strategies tend to provide companies with the opportunity to be discovered by prospects. These inbound sales strategies have earned a legitimate place in the lead generation process, as they enable potential suitors to assess options and make educated choices at their own pace. But even the most beautiful, vivid bird still needs to eat and getting inbound tactics to produce the quantity and quality of leads that are required to feed your hungry sales force can be prove to be quite challenging.
And that's where highly targeted outbound strategies come into play. By recognising your targets and proactively reaching out to them, you're in control of your own successes instead of simply waiting for them to come to you. To make important use of outbound strategies, you should employ the following three key tactics:
1. Fishing In The Right Lake
Targeted prospecting is critical in having successful sales teams. Nobody should be wasting their days calling hundreds of numbers only to come up empty handed. Sale intelligence tools enable you to segment in a wide variety of ways and identify specific targets that will not only have the highest likelihood to convert but also the ones that will turn into high value accounts and long-term customers. With insight into all prospects that use a specific technology, you can select for those whose budgets are big enough to warrant a strategic account approach.
A sales intelligence solution's organisational chart will enable you to target the right people within the organisation but not limit you to just a single contact. Having complete visibility into the IT organisation gives you the power to employ multi-stakeholder selling. This tactic will give you higher visibility into the organisation and tighten your sales cycle as you control when the relevant decision makers are invited to the table.
2. You Can Be Selective
With the adoption of effective sales intelligence tools sales teams can identify and contact the prospects with current needs and reach out only to the prospects with the highest likelihood of purchasing. This eliminates the wasted time and effort of old-school outbound sales. Some might argue that outbound lead generation has gone out of fashion; an obvious reason for this is that outbound lead generation is considered harder and messier "interruption marketing. Cold calling".
Today's sales and marketing teams are no longer looking for the most leads at the lowest cost, but rather identifying and approaching the right targets when informed with solutions designed to address issues that the prospects are currently facing. Making use of tools like Real Time Triggers gives you actionable data about prospects' open initiatives and leadership changes so that you can come knocking when they are prepared to open the door.
3. Follow The Bubbles
Ensure that quality inbound leads are engaged in a strategic manner by outbound sales efforts. Successful people have mastered the art of balance and when we talk about marketing, the discussion always seems to come down to inbound vs. outbound technique. When, in reality, it is far more productive to think about the proper balance between these programs, or how to make them most effectively work in concert with each other for greatest rewards. Inbound and outbound tactics in concert with each other will put you in front of good-fit prospects quicker.
Prospects who download, read and subscribe to your content are actively choosing to know more about your solutions. Supplement the information entered by inbound leads to access content with the complete profile data from your sales intelligence solution. Knowing that the prospect was interested in a cloud storage whitepaper is a good start, but when supplemented with the profile information, your sales team can know before reaching out that the organisation is currently evaluating cloud storage solutions and identify who on the team is responsible for that project as well as have at hand the direct contact information needed to reach them directly and discuss your offering. Being the most prepared vendor when you make the call will is likely to move your solution to the top of the list.
Outbound strategies definitely give you control over the sale. By engaging with prospects early in the decision making process you can establish and develop relationships, provide more tailored solutions and close more sales. And that's what it's all about, right? But it's also important to remember that striking a balance between these two powerful forces of inbound and outbound strategies is crucial for maximising your sales success.


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Our 2016 Program
A note from GWI Founder, Seema Hingorani
Our inaugural GWI four-week Summer Intensive Program on Penn's campus ended on June 24, 2016. Our 30 diverse freshman and sophomores from colleges all across the country learned a lot in the classroom from highly regarded business school professors from Wharton, Columbia Business School and the Yale School of Management, among others. In addition, an incredible lineup of senior women and men in the asset management business spoke to our students throughout the program about their career and life experiences. See the full list of Scholar backgrounds and our professors and speakers. We also took our Scholars to visit a select number of asset management organizations including Bloomberg, DE Shaw, PNC Bank and Wellington Management and ringing the closing bell at the NYSE on June 10th was clearly a memorable highlight!
The curriculum was rigorous and engaging with topics ranging from financial statement analysis and basic accounting to learning how to value a business and construct a portfolio as a public pension fund or university endowment to learning about impact investing and socially responsible investing. There was a full day of presentation skills training and panel discussions about career development and interview preparation. On the final day of the program, each group of Scholars (5 teams) did awesome jobs on their Comcast Corporation stock research reports and PowerPoint presentations. We were all very impressed (especially our Investment Committee members that included myself; Anna Dopkin from T. Rowe Price; GWI's advisory board chair and former CEO of Commonfund Capital, Sue Carter, and Wharton finance professor, Bilge Yilmaz) with each team's investment recommendation to buy or sell shares of Comcast's stock based on in-depth research, including a meeting with senior management of Comcast Corporation sponsored by T. Rowe Price portfolio managers. It was clear that a lot of what we taught our Scholars really stuck! That day we also presented each Scholar with an official certificate of program completion which made for a fun ceremony. We were so proud of our inaugural cohort!
Following our four-week educational program on Penn's campus, our Scholars went on to have spectacular six-week paid summer internship experiences that GWI secured for them at leading asset management firms around the world including Vista Equity Partners, Causeway Capital, Carlson Capital, The Blackstone Group, The Carlyle Group, Oaktree Capital, Taconic Capital, Perry Capital, Wellington Management, Walter Scott Partners Limited, CPPIB, Bowdoin College Investment Office and MassPRIM. See the full list of internship firm partners here. Our students were in close contact throughout their internships with GWI staff and, importantly, with our GWI Ambassadors, women and men in the asset management business who served as local, on-the-ground resources and friends for our Scholars in the regions where they interned. These Ambassadors were there to help with any questions and to offer advice. Many of them took our students out for lunches and dinners to get to know them and make them feel at home, which was wonderful. See the full list of our GWI Ambassadors here. The relationships and friendships that our Scholars formed with so many amazing people during their ten weeks this summer will be invaluable to them for the rest of their lives.
Seema Hingorani
Founder and Chair, Girls Who Invest


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This is the perfect tour for rock 'n' roll, blues, and country music lovers. Enjoy city sightseeing in Memphis and an orientation tour of Nashville with a stop in Tupelo, Elvis' birthplace. Begin your trip in Memphis and visit the Rock 'n' Soul Museum; the Stax Museum of American Soul Music, a tribute to the great Memphis soul musicians; Sun Studio, probably the most famous recording studio in the world; and Graceland, where you experience life as Elvis did. Head east to "Music City USA"—Nashville—for reserved premier seats at the world-famous Grand Ole Opry. Visit the Johnny Cash Museum to see his historical documents, awards, costumes, and instruments. Step inside Studio B, Nashville's oldest surviving recording studio and one of the country's most important studios, and go behind the scenes of the music industry at the Country Music Hall of Fame. Another special treat awaits: a private performance and discussion with John Carter Cash, Johnny and June Cash's only son. Fans from all over will appreciate the heart and soul of this musical journey.
City Highlights
Sightseeing: In Memphis; orientation tours in Tupelo and Nashville, Stax Museum, Rock 'n' Soul Museum, Sun Studio, Elvis Presley Birthplace & Museum, Graceland, Johnny Cash Museum, private performance with John Carter Cash, Country Music Hall of Fame, Studio B, and reserved premier seating at the Grand Ole Opry. Inside visits as shown in UPPERCASE in the tour description, including admission charges where applicable.
MEMPHIS Crowne Plaza Memphis Downtown (MF) & Elvis Presley Heartbreak Hotel (for early 2016) (MF) / Guest House at Graceland (for late-fall 2016 departures) (MF)NASHVILLE The Inn at Opryland (MF)
Transportation:Private, deluxe air-conditioned motorcoach while touring.


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Read More on How To Download YouTube HD Video Free On Mac pc (Macos Sierra) »]]>
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I wager you're thinking, that you somewhere have to paste it, but no. This is already taken care of even. You shall start to see the icon of the video inside the MP3 YouTube Converter. In fact this small software makes a huge youtube to mp3 hd difference to every iPhone/iPad user that spends enough time on YouTube. Download , install & run Free YouTube to MP3 Converter Find the songs you want to download for your iPhone using the search bar. Read the detailed comparison of YouTube Red and the recomended application here: How to Get YouTube Red free of charge.
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Read More on Koh Samui's Ideal Seashores Uncovered »]]>
We strongly allude to that you appoint a house real property agent gold-colored expense business home till subsidize you with the buy of your Samui Expletive Villas. Whereas they've worked well several instances before re also Samui Overlap Villas, they comprehend to whom straight to proceed to under what issue. Every inbuilt yourkohsamuivillas house buyer in Thailand is definitely destined to deliver taxes on rent unearned income. Thailand will not consider a unique capital disposable income taxes for international traders. Samui Extravagance Villas are separately embosomed and assure yourselves enjoyment that your increasing generation mood always keep in mind.
They also offered good suggestions on regional locations and suggested great actions to perform in and outdoors of Samui.
The concierge team will then be at your service, arranging and organizing everything before your arrival from the comfort of your own home, Guiding you through the entire length of your stay, From a visit to a world class Health spa to a private plane to deliver you in style to your beach front wedding. Centered from our gorgeous centrally located workplace in Fishermans Town, you will advantage from our 10 years of encounter on Koh Samui in all elements of High-class properties Kalara is definitely the Bloomberg " Greatest Agent for Thailand" champion 2011 and is definitely one of the just businesses in Thailand to keep the desired AIPP regular membership.]]>
Read More on Mab Prachan Koh Samui »]]>
We have 30 golf courses withing 1hr driving – WORLD RECORD! The closest is Siam Country Golf Club. Laem Chabang International and St. Andrews are designed by Jack Nicklaus. This makes our place an excellent choice for your Koh Samui golf holiday.
We offer 28 beautifully appointed rooms, a large SALTWATER swimming pool in the garden, free wireless internet, and a superb air-conditioned pub with large TV screens. In addition an outside restaurant offering a wide choice of food and drink, snooker tables, a movie theatre and a gym and sauna for a perfect relaxing evening.
Samui offers a fascinating world of parties and night life, probably the best you can find in Thailand!
Koh Samui offers a fascinating variety of touristic highlights, absolutely worth your visit: The Sanctuary of Truth (world's tallest wooden building).
The Silverlake vineyard area with the laser Buddha, thai and chinese tempels.
Koh Larn Island with its beautiful beaches which costs you 1 Dollar for the ferryboat.
Sri Racha Tiger Zoo, if you are not afraid to caress a baby tiger.
Shopping, shopping, shopping in Samui's shopping tempels or open markets.
Koh Samet, a small island famous for its amazing beaches.
Saltwater Pool and Outdoor Jacuzzi (even for left menu)
Our 22m long saltwater swimmingpool is the center of the property. No agressive clorine in the water – you will feel like swimming in the ocean, only much cleaner and with a drink standing on the side of the pool. The small and gentle wind which passes slowly through our gardens helps keep you cool whether you just take a relaxing swim or some healthy exercise. Just perfect after a day on the golf course or when you come back from work in the Eastern Seaboard. !
We serve food and beverages at our garden bar, right besides the pool, and at our poolside tables.
On Samui's largest TV screen we present movies and all kind of life sport events, which you can watch from your table as well as directly from the swimming pool]]>


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CONCORD -- As it has done in the past, New Hampshire is once again leading a pack of states in taking a stand against intrusive government.
The New Hampshire House of Representatives is presently considering HCR6, a bill stating that New Hampshire will no longer recognize the authority of Washington, D.C., if the federal government violates the U.S. Constitution in specific ways. Among these are the institution of mandatory national service, military draft without a formally declared war, unconstitutional implementation of martial law, or further restrictions on gun ownership.
The bill enjoys bipartisan support and is also supported by many members of the Free State Project, an effort to move liberty-loving people to New Hampshire, where they will work to reduce the size and scope of government.
The bill is sponsored by NH State Representative Dan Itse (R-Fremont), who also authored legislation condemning the USA PATRIOT act. "The present bill has tremendous popular support. The members of the Free State Project have helped to spread the word about this resolution across the state", said Rep. Itse. "That's a big step in moving this forward."
This type of legislation has precedent in New Hampshire. In 2007, the state formally withdrew from the federal REAL ID program. Many participants of the Free State Project were heavily involved in that effort as well.
Representative Itse will be a speaker at the Free State Project's Liberty Forum, held from March 5 – 8 in Nashua, NH. Rep. Itse will be joined there by Free State Project member Rich Heller, who successfully overturned the Washington, D.C. gun ban in the DC. vs Heller Supreme Court Case.
The group's website is freestateproject.org <http://freestateproject.org>


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This step is elective but will seriously raise your chances of getting noticed by other people on the website. Write a little something about yourself, your hobbies and upload some good photos of yourself.
Utilise our website's advanced search to locate members who match your criteria. Or just flip through listings and locate singles that take your fancy.
Located someone who meets your requirements? Send a letter to them or just a wink and see what comes of it. With a little luck you'll be on your way to finding your suitable partner.
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Hey everyone I'm Victor from Brazil, just finished my environmental engineering graduation and going to study 10 months in Brisbane. While graduation student I lived in a student house with about 50 other mates for 5 years. There we were the only responsables for keeping the house, so I'm used to work together to do all stuffs and live in harmony. I'm a chill guy, like to talk, share and learn new experiences. Love travelling and meeting new cultures.
Hi ! I come from France and I study Management of Sport at University of Strasbourg. I enjoy hanging out with friends, make party, going at festivals and enjoying life ! I'm little bit maniac but I think I'm easy-going and fun too. I go in Australia for 3 months break during my last school year.
Helloo, Max an i are a couple of well mannered rural folk from north west Victoria. Hahaha rural folk. Yeehaa we'll teach you how to kick up the dust and lasso a horsee. Hahaha nah, not quite. We're both vegan and love to eat and cook, alot. Max is fully qualified in Pest control management but has been pursuing one of his passions and working as a Spare parts interpreter for a Harley Davidson dealership. I've been a Barista at the same establishment for 9 years. Hoping to pursue my own passion and undertake study in Psychology in the near future. Max was born in Brisbane and has forever told me how wonderful it is to live there so we've decided to pack ourselves up and both make the big move. We're both friendly, respectful, honest, clean and highly conscientious people. Very social, reasonable, easy to get along and communicate with but both understand boundaries and alone time. We both love gaming, gardening, diy, cooking and outdoors adventures along with many other things. We'll make great roomates and great friends too.
I'm a chill guy from Norway, working part/full time as a marketing consultant in Brisbane. This is the start of my third year in Brisbane, since 2012, just can't stay away! Looking for a place close to the city centre, south side is a plus. I'm also in need of furniture, but a bed is enough :) Love a few drinks and doing social things, but does also respect others when it comes to respecting "quiet time" and keeping things clean. I'm also a person that loves movies, gym and tavels. Ps. I am most likely in need of a place I can park a motorcycle 8-)
Hi there! We are an Argentinian couple looking for a place to stay in Brisbane for almost 3 weeks. We are glad to share time with other people!
Hey there everyone :) I'm a new educated multimedia designer from Denmark, who had my internship in Melbourne for 10 weeks earlier this year with 'burgersofmelbourne' - and I'm coming back to Australia at the end of August! I loved it here and I love the Australian people, so I'm super excited to come back. I'm looking for a place to stay. I'm very friendly, polite and take the owner and roommates needs and wishes into account always! :) I'm outgoing, fresh and always up for having a good time or chilling with a movie on a Sunday in pajamas! I would love to hear from you guys!
Hi, my name is Nadine and I´m from Austria (the country without kangaroos). I am 24 years old and I gonna be an exchange student in Brisbane next semester. I am looking forward to find new friends in a lovely shared accomodation like yours. I prefer having my own room with an aircon system that can do heating as well. I can be a very communicative and adventurous as well as a quite and regardfull person, especially in the exam period. In my freetime I love to dance, so if you want to learn how to salsa or maybe viennese waltz this would be your chance. :P If you do have any further questions feel free to ask. Looking forward meeting you! Cheers Nadine
We are a friendly couple from a small town in North Queensland! We are looking to relocate to the big smoke early September in order to discover the ways of city living. I work in hospitality and Jack is an electrician and we hope to secure jobs before the move HOWEVER if we are not yet employed there is financial backing for this exact moment in our lives that has been in the making for quite some time. We are determined to begin the next step in our lives and would be very grateful if someone would give us a chance. My name is Becky (23) and I love meeting new people and making friends, My day to day routine includes working, reading, watching tv, cooking, listening to music, petting animals and on the weekends I like to go on adventures! I love trying new food, watching movies and just exploring the city. I am a huge Harry Potter fan and my favourite band is still Fall Out Boy but don't hold that against me. I am extremely easy to get along with and I am very respectful of others :) Jack (25) is easy to get along with and can cook up a storm, so we wouldn't mind sharing meals occasionally.. :) He is absolutely hilarious and will tag you in the dankest memes. He does a bit of reading and a lot of binge watching tv shows. He likes to have a few drinks here and there mainly on weekends but is completely respectful of others. Together we are just looking to find a place to live and make some awesome friends!
Hi there! My name is Daisie, 25 year old vegan. Seeking a one bedroom place for myself and my very well behaved 7 year old staffy. Alternatively a temporary (potentially longer term) room to rent while we find a place of our own. Preferably Brisbane or the Gold Coast. Even something some where in the hinterlands would be cool! If you think you might have something suitable for us we would love to hear from you! Warmest regards, Daisie and Whoopsie!
Easy going int student. Tidy and nice person.
I am clean and tidy. Easy going.
We are clean and tidy we are both quite people and nice people
Hi I'm Ashleigh, I'm looking too move to Brisbane to be closer to work opportunities & family. I currently reside at the gold coast. I am unemployed at the moment but eager to get back into working as soon as I settle into stable accommodation in the Brisbane area. In saying that i also study online, im currently completing my 1st year doing my bachelor of social sciences. I'm easy going, get along with everyone. I'm a friendly single, 25 year old female keep to myself normally, very clean, drug free. Would like to find room that also has own bathroom but share is okay. Internet is a necessity. Great rental history. Refrences can be provided. Able to pay bond & 2 weeks in advance if required. Mainly Looking only for a female flat mate but a couple is fine.
Very easy going and tidy, enjoy making new friends and meeting new people. I work about 60hrs a week in sales and generally come home and then go train, I enjoy relaxing and watching movies when home or heading out to the glasshouse mountains for some rock climbing on my days off. I'm a non smoker, and consider myself respectful and relaxed. Just be nice to find a great place with a nice housemate. I require a place with secure parking and Also own bathroom is preferred
I am a brazilian guy with japanese ascendence. Organized, clean, easy going and student @ TAFE (tue,wed,thu). I can cook and we will have a great time living together. I am not a party guy, but I don´t refuse a cold a beer. Cheers
Hi there, I'm Chris from Berlin, Germany. I just moved to Brisbane on the 27 of July as an exchange student at the QUT (Gardens Point). Staying in a hostel a.t.m. and looking for a nice flatshare for the next 4 month. I'm an open minded and communicative person and lived in a flatshare (male and female) for the last 4 years, so I'm used to cook, having a beer (German stereotype - check! ;), go out dancing, doing sports or just hang out, together with my flatmates. But I also totally understand if someone wants to be alone or just needs some rest. I'm also a clean and tidy person and I like animals, so if you have a pet, this shouldn't be a problem, except it is something creepy! :D Some more about me? For the last 6 years I worked as an event manager (in full-time) for the cultural department of one district office of Berlin. Next to my job I studied in the evenings for the last 2 1/2 years. But now I quitted my job to be able to study abroad for one semester and go on traveling afterwards. I saved enough money and will also try to get a job in bar or sth. similar, so no worries, I will be able to pay my rent easily (even without the job)! If you have any questions, just hit me up! Cheers Chris
Hi everyone, I am a very neat and tidy person. I'm very respectful of others and their need for quiet and personal space, I'm also mature, independent, understanding, fun loving, social and friendly. I'm 18 so I'm always keen for a good night out but I also I like catching up with friends, I'm an active person but I do love my food and I also like chilling at home watching some good old Netflix! Right now I'm on my gap year working on our family property in NSW. I am a farm girl but don't worry I'm not a complete bogan and I went to boarding school in Brisbane so I know my way around and have plenty of friends up there. I'm hoping to move in late July or early August so I'm able to settle in and get a job and earn more money before I start uni at QUT next year. I'm looking for a place that has easy access to the city, is preferably furnished but if not that's ok, I love animals so I'm more than happy to live with pets and I am looking for a place where the rent is under $180! So if you think that I would be a good fit for your house please message me!!!
Thought I'd create an Ad seen as I may not be renewing my current lease. Looking for a private room with en-suite bathroom & secure car space please. I'm a 24 yr old female, originally from New Zealand & am employed full time. Clean & tidy, laid back, loyal, down to earth, mind my own business but won't turn down an arvo beverage kind of girl. Also into health & fitness.
I'm from Chile, and I came to Australia to study English.
I am IT professional working full time in Brisbane city. I am looking for separate room in shared house somewhere near to city. I don't drink alcohol and also don't smoke.
Norwegian student planning to study at UQ
I am very friendly and open mind. I love to live in a clean Space ;)
Hi, we are a French couple in Australia since 9 months. After many road trips in our camper van we would like to work in Brisbane and find a nice place to leave :)
Hello !! We are two people, Marion (22) and Clément (23). We are looking for a roommare, for a budget of 200$ per week. We like to chat with other people, we love cooking and finding new recipes.


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Dr. mark Birnbach
boulder, colorado - USA
No approved endorsements
About Dr. mark Birnbach
I practice general and cosmetic dentistry in Boulder, CO. I have experience and skill in the field of dentistry. I have taken over 3500 hours of continuing education. I have completed hands-on esthetic courses with internationally known cosmetic dentists Bill Dickerson, Larry Rosenthal and David Hornbrook and conducted dental seminars on esthetic dentistry and thrice been awarded "Clinician of the Year" by the Boulder Dental Study Club. I have been recognized as an expert in the field of TMD (More about TMD – Temporomandibular Joint Dysfunction), the treatment of chronic head, neck & facial pain.
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The Redskins on Sunday face a Cleveland Browns team that has won their last three games and four of their last six.
Part of the reason for the surge has been the improvement of the Browns' young offensive players, Redskins defensive coordinator Jim Haslett says.
As they set out to rebuild their team this year, the Browns looked to upgrade their offense and drafted running back Trent Richardson third overall and quarterback Brandon Weeden 22nd overall. Right tackle Mitchell Schwartz was the fifth pick of the second round and then in the supplemental draft the Browns took wide receiver Josh Gordon.
All four start on offense along with another young player, second-year wideout Greg Little.
Those players have helped Cleveland's offense average 23.3 points a game after averaging 17.9 points through their first 10 games.
"I think they're getting better and better every day," Haslett said. "I'm talking about the receivers, the quarterback, the running back. Obviously, [Richardson] was nicked up early. They have a really good offensive line – a young offensive line. They're a good football team. They've won a bunch of games lately, and they've been in every single game. This will be a good challenge for our football team."
Richardson, who is playing with a rib injury, has rushed for four touchdowns in the last three games after managing five over the first 10 outings. Gordon – Robert Griffin III's former Baylor teammate – has hauled in a team-high five of Weeden's 13 touchdown passes.
And on the win streak, Weeden has posted two of his three most accurate passing performances.
Despite the promise that the young Browns have shown on offense, the unit has struggled significantly with ball security. Weeden has thrown 15 interceptions – tied for fourth in the NFL. Meanwhile, Richardson has lost three fumbles.
The Redskins' defenders, who have recorded 15 interceptions and nine fumble recoveries, believe that the opportunity will present itself to increase those totals this week, and that that will be key to their success.
"Forcing turnovers, and turnover differential is always a key stat and it's definitely something we thrive on as a defense," nose tackle Barry Cofield said. "Any time you have a rookie, and I think Richardson has a couple fumbles also, when have a team that turns over the ball, it's a copycat league. You want to see what their problems were in the past, and if turnovers are it, that's something we're going to harp on."


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Add Kettering (Ohio) Alter signal caller Malik Zaire to that list after a very strong junior season.
Already with a handful of offers, Zaire, who stands 6-foot-1, 190-pounds, has a strong arm with the ability to extend the pocket to find an open receiver.
With early offers from Ohio State, Wisconsin, Cincinnati, and Northwestern, Zaire is sure to be a quarterback Michigan State takes a hard look at.
Here is Part One of the final highlights we received.


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by Layken W
I luuuv horseland! I go on it everyday and I have met so many great friends! Best of Wishes,Layken
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