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|Tipo de imóvel
|Estadia Mínima
|4 - 8 noites
NÚMERO eleito um BEACH HOUSE EM NÚMERO UM PRAIA EM COSTA RICA !!!!
Aninhado entre 2 acres de vegetação tropical e belas flores tropicais, sobre o que é dito ser a mais bela praia na Costa Rica, é Casa Piedra. Ouça as ondas quebram na praia, nadar na piscina de borda infinita, cheirar as flores tropicais, ouvir os macacos, desfrutar os beija-flores, borboletas e da preguiça ocasional tudo em total privacidade. Tome o seu café da manhã ou suco fresco em nossas cadeiras de balanço confortáveis da Costa Rica, (e os nossos pôr do sol fabuloso também), ouvindo e observando as ondas ir à praia de areia branca.
O terraço é coberto ainda totalmente aberto e totalmente privado. Nossas árvores de fruto incluem, limão, manga, manga, abacate, grapefruit, laranja, guabana vários tipos de bananas e um casal com o qual você provavelmente ainda não está familiarizado. Temos também orquídeas e cocos.
Casa Piedra é ideal para uma lua de mel ou uma pequena reunião de família. Seja como ativo ou privado como você escolher.
Casa Piedra 8 pessoas em 3 quartos. O mestre tem vista para a piscina e para o mar. Todos os três quartos têm camas queen size. A casita também tem 2 camas de solteiro.
Nós temos a equipe mais simpática casa na Costa Rica. Olivier vai buscá-lo e devolvê-lo ao aeroporto e ajudá-lo a organizar você aventuras locais. merissa vai lavar e passar suas roupas e ter o seu café da manhã pronto quando você acorda. Eles estão aqui para ajudá-lo e garantir a sua estadia é o melhor !!!
As atividades são infinitas, se você optar por participar. Rafting, cavalgadas, mergulho, tirolesa, golfe, surf, ver nidificação de tartarugas e praia pentear para citar alguns. A pesca esportiva está disponível para sailfish, marlin, atum, wahoo e muito mais. Então, claro, há snorkeling. Faça uma massagem maravilhosa no terraço enquanto ouve as ondas, vamos organizá-lo. Caminhar até a praia e desfrutar dos restaurantes em Flamingo. Desfrute povo da Costa Rica, as aldeias vizinhas, desfrute Casa Piedra. Será um período de férias você não vai esquecer.
Casa Piedra tem sido a nossa casa de família por quase 20 anos. É um tesouro isolada extremamente confortáveis !! É diferente de qualquer outra casa na Costa Rica. Nós sabemos que você vai se divertir tanto quanto nós fazemos.
Palavras-chave: Casa de praia
The house and location were fantastic. The place was even better than the photos showed. The beach was mostly secluded, the waves perfect for adults to body surf or just play in. The view from the expansive deck was just what I was looking for. Many an hour were spent on the deck or lounging in the infinity pool. Walks and runs on the beach, eating at Coco Loco's, reading on the deck with the waves crashing in front of you - a wonderful place to vacation. It was also close enough for my adult sons to go to Tamarindo for some very big waves and a day and a half trip to Arenal to see the inland terrain and do some hiking. I highly recommend this spot!
We booked the home for a family vacation all adults. 6 in total. We found the set up was perfect as the rooms being separated gave us all our own space. We found we all gravitated to the beautiful infinity pool as well as the patio all the time. The view of the beach and the ocean was fantastic. The surf was great, beach is secluded and not crowded. The proximity to Coco Loco (local eatery) was advantagous as their menu was great. The staff Oli and the daily visits of Maritza helping out to keep the property clean and attend to our needs was welcome. Oli and Maritza are true professionals. We highly recommend this property. It can be perceived a little rustic but this is the charm of this home. We Would book again again.
This is an amazing property because of its view, location and proximity to the beach. We were there during Holy Week and there were activities set up on the beach with a grand stand and a band and other fun things - but it shut down early - like 9:30pm - so it never disturbed us too late. The beach is a pristine white sandy beach - with no seaweed when we were there. There were huge waves that were a blast but that would have made me nervous if my kids were younger. There were many restaurants within walking distance but if Oli is available - he would drive you if you want to go further. He was the best when it came to problem solving or a quick trip to the grocery. He set up a fishing/snorkeling private boat which was terrific too.
The sunsets and lanai were absolutely breathtaking. Also, the moon over the water late at night is incredible - we were there for a full moon and it was truly spectacular.
The only draw back of this fabulous property is that the third bedroom is sort of like a coach house and set back from the other two bedrooms - which each have their areas as well. If you are thinking about having young children share the double beds room - am not sure it is the best fit. They would be set back from you and with the beautiful ocean noises, you probably wouldn't hear if they needed something. My youngest is 14 and she was fine there with a friend but I just was thinking if they were younger, I would have had an issue.
Another plus, Oli set up a chef to cook some dinners and lunches for us and it was a great way to relax at this beautiful spot. He also had some ladies come and give us fabulous massages on the lanai! It was one of the many highlights of our Costa Rican trip! Also, Mareetza (sorry if I spelled it incorrectly) was a dream since we came from the rain forest and she did all our laundry and kept the place nice and neat. She is a gem. I hope you get a chance to check out this special spot!
Our family of 6 adults stayed for a week. The location was beautiful and accommodations were very good. Even though there is a road between the house and beach, it is a quiet location and there is a mile of beach to explore. Good restaurants nearby including Coco Loco next door (excellent). Staff at the house were great; Olivier and Marista were very attentive and helpful. My only complaint was the lack of communication from the owners prior to our stay. On several occasions there was no responses to my phone messages/e-mails. The owner responded to the VRBO site communications only.
The six of us ladies just got back from a week at the most beautiful beach. Our villa had a view that can never be outdone. We spent hours just sitting in the pool and on the large deck staring out at the Pacific Ocean. The location of the villa is second to none on that entire beach. And of course, thanks to house manager (Oli), we were able to do many truly memorable activities. Oli was able to set us up with great events and people, maximizing the entire Costa Rican experience. We all could not have been happier with the location, the view and the staff!!! Great time had by all.
We just spent the past week in paradise. The accommodations were great and fit all of us (4 adults and 4 kids) perfectly. We loved the openness and the large outdoor space overlooking the ocean. It was the perfect place to watch the sunset every night. The kids got a lot of use of the pool and loved taking the boogie boards down to the ocean. The staff was very friendly. Olivier checked in with us every morning to see if we needed anything and Marissa ensured the place and our clothes stayed clean. The entire property is gated and has a security guard on site at night, so we always felt safe. Whenever we didn't want to cook for ourselves, we would venture out to one of the local establishments. A few that we visited and enjoyed were Coco Loco (right next door), Maries and the Monkey Bar both of which were about 600-700 meters away.
The only two minor hiccups in our stay were the 24 hours where the water was shutoff for the entire town and our last night when the air conditioner stopped working in the casita where the kids were sleeping.
We would definitely stay here again and recommend it to anyone that is looking for a house on the beach in Costa Rica.
Turtle visualização do assentamento, tirolesa
|Validade da Tarifa
|por noite
|Noite (fim de semana)
|por semana
|por mês
|Pacote ou Evento
Off season
mai 1 2017 - out 16 2017
Estadia mínima: 4 noite(s)
Christmas holiday
dez 24 2017 - dez 31 2017
Estadia mínima: 8 noite(s)
Taxa Única
New Years holiday
jan 1 2018 - jan 7 2018
Estadia mínima: 7 noite(s)
Taxa Única
Tarifa Básica
Estadia mínima: 4 noite(s)
For holiday rates contact owner


text:
Business Card for an airport
Budget €250-750 EUR
we are an airport and we need some idea designs for logo and business card
135 freelancers are bidding on average €308 for this job
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здравейте, казвам се Тодор Тодоров и бих искал да работя с вас върху проекта за лого и визитка. моля ако се интересувате от услугите ми посетете www.f5designstudio.com където можете да проверите нивото на предлаганите More
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subset: commoncrawl


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Miniature hairs appearing along former hairline after traction alopecia?
I'm a 18 year old male that suffers from mild trichotillomania due to depression. I've seen a psychiatrist and have stopped the self-destructive behavior, though much hair around my temples has already fallen out. I have began to apply Minoxidil to the temples twice a day, and, after three months, I'm beginning to see small, blond (I'm dark-headed) hairs appear along my former hairline. Will these develop into full hairs?
Doctor Answers 1
Hair growth
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Thank you for your question. I cannot fully answer your question without seeing your hair or photos, but it sounds like the small, blond hairs you are seeing are vellus hairs. Vellus hairs are new hair growth and depending on the cause of the hair loss, they grow in extremely fine and light colored. Over time, they may or may not regain color and thickness. I highly recommend you consult with a board certified dermatologist to have your hair and scalp evaluated. Take care and good luck.
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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Product Spotlight
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Kroger Posts Strong Q4, Year
CINCINNATI - Beating analysts' expectations, fourth quarter and full year results released yesterday by The Kroger Co. here proved that its efforts to heighten the shopping experience with improved selection, enhanced customer service, discounts, and better store conditions are paying off handily.
CINCINNATI - Beating analysts' expectations, fourth quarter and full year results released yesterday by The Kroger Co. here proved that its efforts to heighten the shopping experience with improved selection, enhanced customer service, discounts, and better store conditions are paying off handily.
"Kroger delivered consistently strong results during 2006, exceeding our original guidance for both identical supermarket sales and earnings per share," said Kroger's chairman and chief executive David B. Dillon yesterday. "These results and the gains we have made in market share throughout the year indicate that Kroger continues to compete effectively in this challenging environment. Our associates' commitment to our plan will continue to enable us to deliver the service and value our customers expect."
The leading supermarket chain said that during the fourth quarter, ended February 3, 2007, it tabbed a 14.5 percent gain of $16.9 billion, up 5.7 percent after adjusting for an extra week, and a 5.6 percent same store sales increase including fuel.
Quarterly earnings, meanwhile, rose to $384.8 million, or 54 cents per share, up from $282.1 million, or 39 cents per diluted share in the fourth quarter of 2005.
For the full 2006 fiscal year, total sales increased 9.2 percent to $66.1 billion. After adjusting for the extra week in fiscal 2006, total sales increased 7 percent over fiscal 2005, while same store sales increased 6.4 percent with fuel and 5.6 percent without fuel based on a 53-week period in both years.
Net earnings for fiscal 2006 amassed $1.11 billion, or $1.54 per diluted share. Kroger's fiscal 2006 summary included a 53rd week that benefited the year by an estimated $0.07 per diluted share, as well as $0.03 of expense per diluted share for legal reserves recorded in the first quarter vs. 2005 net earnings of $958 million, or $1.31 per diluted share.
In a conference call with analysts, Dillon said while the company's quarterly progress was largely in line with expectations, "five or six things" worked especially well together that produced "an incredibly strong quarter." In addition to solid same store sales gains, better-than estimated gross margins, and depreciation, Kroger also made strides with its natural and organic offerings.
When asked about changes and opportunities for improvement at the midpoint of Kroger's five-year strategic plan, Dillon said while the company didn't get quite as much accomplished as it originally anticipated, it anticipates "strong future sales growth opportunities" by remaining focused on staying "meaningful to our customers....and making sure that the stores we offer are relevant" to each individual market.
Dillon said customer loyalty and data from its loyalty program analysis partner dunnhumby will continue to play an increasingly important role in Kroger's game plan, as will managing perishable shrink in meat and produce. "Shrink in fourth quarter showed improvements vs. the year before in perishables," he said, noting, "there's still good room for improvement," particularly as it relates to establishing the root causes of shrink.
"If incremental shrink in produce was in organics, that's one thing," but if it is stemming from traditional categories, he added, "that's quite another." To that end, Dillon said Kroger will seek to better identify "where to expand and where to contract to benefit sales."
Further, while the first, second and fourth quarters of 2006 presented significant deflationary conditions that hampered fresh meat category sales, Kroger caught especially strong seafood sales, Dillon noted.
Dillon further credited Kroger's workforce for contributing significantly to its strong fourth quarter results, which he said demonstrates "that our associates understand the importance of offering customers improved service, product quality and selection, and value. Our performance last year is the result of the hard work and dedication of our associates."
Kroger has a $1.9 billion to $2.1 billion capital expenditures purse to work with this year for its larger, faster growth markets, while it plans to grow supermarket square footage by 2 percent before acquisitions and operational closings
When asked whether acquisitions were on the drawing board, Dillon said it's "not for lack of trying," but rather price differentials between what is available and what Kroger is willing to spend. "We continue to see a lot of things out there," but in the interim, will continue focusing on "helping grow our market share in some markets" via expansions and major remodels, he said.
In his year-end review, Dillon said Kroger gained share in 36 of its 44 major markets, based on internal company estimates. Kroger increased share in 26 of 32 major markets in which it competes directly with Wal-Mart Supercenters. Its share declined in five and was unchanged in one. Dillon said Wal-Mart added 125 Supercenters, giving it 1,000 units in Kroger's major markets.
During the analyst call, Dillon refrained from commenting on what is expected to be challenging labor negotiations the grocer has ahead with unionized employees in Southern California, Cincinnati, Detroit, Houston, Memphis, Toledo, Seattle and West Virginia this year.


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|Shinobu Kawai <shinobu.ka...@gmail.com>
|Re: EscapeTool (and velocity-tool best practices)
|Wed, 08 Dec 2004 21:00:19 GMT
Hi Mike, > I started using EscapeTool today (since it was far more comprehensive that my > primitive and misnamed URLEncoder tool), and noticed that it only took > String arguments. Oops. > In general, should Velocity tools that operate on a String operate on an > Object instead and perform a object.toString() conversion first? Or at > least provide Object argument wrapper methods to call the String argument > methods? Sounds reasonable to take Object parameters. Quoting Nathan, "Java, being strongly-typed, distinguishes many things that VTL tries not to distinguish". Just be sure to check for null. ## But hey, RenderTool takes only Strings for VTL. > Obviously, I can easily subclass EscapeTool even if no one else agrees with > me and add "public String html(Object object)"-like methods, but I'm curious > what we consider to be best-practices.... Overloading in Tools currently gives strange behaviour. http://issues.apache.org/bugzilla/show_bug.cgi?id=13637 http://issues.apache.org/bugzilla/show_bug.cgi?id=16707 ## are these duplicates? Best regards, -- Shinobu Kawai -- Shinobu Kawai <firstname.lastname@example.com> --------------------------------------------------------------------- To unsubscribe, e-mail: firstname.lastname@example.com For additional commands, e-mail: firstname.lastname@example.com
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Go off the grid with our experimental
learning program in Patagonia, Argentina.
Road2Argentina hosts this unique program in partnership with a sustainable living project located near El Bolson, Argentina – a small, friendly mountain town in the Rio Negro Province of southern Argentina which boasts the Rio Azul Protected Natural Area, part of the largest UNESCO Temperate Forest Biosphere Reserve in the world, as its backyard.
The project was conceived in part to carry out the mission of the Land Ethic Action Foundation (LEAF), a not-for-profit organization formed in 1994 with the goal of teaching and demonstrating self-sufficiency and sustainable development. By implementing the techniques developed by John Jeavons of Ecology Action, the project has
succeeded in bringing the benefits of Biointensive Mini Farming to the Southern Cone region of South America.
The project sustains itself in part by operating a sustainably built wilderness lodge which employs members of the local community and welcomes travelers from around the world to enjoy farm-to-table meals, outdoor recreational activities and opportunities for authentic cultural interaction in Patagonia. While the lodge is undoubtedly an integral part of this project, volunteers play a vital role in helping this experiment in sustainable living achieve continued viability year after year.
As a participant in this hands-on program, you will expand your knowledge of sustainability topics and local efforts to preserve and protect the natural environment that makes Patagonia such a special place. Through direct involvement in organic farming and gardening practices and environmental conservation initiatives, you will learn valuable skills while at the same time helping this pioneering project advance and meet its goal of sustainability.
Participants are expected to spend 6-7 hours per day Monday through Friday volunteering in the core areas described below. Weekends are free to visit downtown El Bolson and La Feria Regional Artesenal or explore and enjoy the Patagonian wilderness which surrounds the program site on all sides.
Tasks include preparing beds, seeding, transplating, weeding, irrigating, harvesting, making compost, caring for the farm animals and assisting with ongoing construction projects, etc. Participants may also lend a hand to the chef in the kitchen, preparing fresh, organic meals for guests.
Tasks include removal of non-native species, trail building and maintenance for low-impact use, as well as construction of a circuit of trails, bridges and campsites in cooperation with local organizations committed to promoting sustainable tourism in the surrounding natural area.
Tasks include working with local schools to develop or remodel greenhouses and gardens during the academic year, and lending a hand to the community with ongoing projects such as building a community hay barn, rebuilding barns and sheds, and helping neighbors with the harvest.1
1Please note that a limited number of community outreach projects may be available between December and March when schools are on summer vacation and many locals are dedicating their time and energy to the seasonal tourist activities which provide essential revenue for the community.
An extensive system of trails and refugios, or mountain shelters, which lead you through the spectacular mountains and glaciers of the Patagonian backcountry, is literally at your doorstep. Recreational activities including kayaking, whitewater rafting, mountain biking, canyoning and horseback riding are also available to interested participants.
The following items are included in the program:
Daily meals prepared with fresh, local ingredients
Welcome orientation and tour of the project site
Dormitory style accommodations
Transfer in and out from the bus terminal to the project site
Bus transportation from Buenos Aires or transfer from Bariloche (BRC)
Weekly visit to downtown El Bolson and La Feria Regional Artesanal
Volunteering activities including organic farming and gardening, environmental conservation and community outreach projects
Recreational activities including kayaking, whitewater rafting, moutain biking, canyoning and horseback riding can be added on to the program at an additional cost
Support from the Road2Argentina and on-site project staff


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Hotel Trees are unusual trees that grow in the Metal Forest in the Nome Kingdom.
They bear a certain kind of table d'hote fruit called "Three Course Nuts", which are something like coconuts to look at. Unscrewing the top of the nut reveals a cupfull of good soup. The middle part contains a hollow filled with meat and potatoes, vegetables, and a fine salad. The last section contains pie and cake, cheese and crackers, and nuts and raisins. The Three-Course Nuts are not all alike in flavor or contents, but they all are good.
Breakfast Trees are another variation of Hotel Trees. They contain coffee or chocolate instead of soup, oatmeal instead of meat and potatoes, and fruit instead of dessert. (Tik-Tok of Oz)
These Hotel trees are similar to the Dinner-Pail Tree and Lunch-Box Tree from the Land of Ev, which L. Frank Baum introduced in Ozma of Oz.


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I wonder how Chris Mathews will hold up on election night? He had major problems after Obama's disasterous debate. How will he cope with losing the election????
I hope that he does a Hari-Kiri live during peak viewing hours.
Chrissy will have a meltdown on national TV. He will then be off the air for a much needed vacation.
About a week after the election there will be a small news story on CNN about a "former news anchor" named Chris Matthews who tragically committed suicide. It will be reported as a sign of mass depression taking hold in the United States because of our corrupt election process, of course. Back to you Wolfe...


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
Now they want me to do proof of service? I looked it up there are so many forms. Form 30, form 40 and many different. Which one do I need?
I am not sure you want a rehearing, but it sounds like you do want to present a defense of some sort to the ticket. If you have no defense, stop reading.
Assuming you have a defense that you want to present, I would speak to the clerk in the courtroom where you were supposed to be, but were not due to the medical emergency. Show the clerk - hand her - a photocopy of the medical records from the ER that shows the condition was a medical emergency. The clerk should then talk to the judge. These things do happen, but many people are not truthful about such an excuse. Bring documentation to show the clerk (not just a filing window). Ask if the clerk will allow you to do an oral motion for "nun pro tunc" to turn back time to the date of the hearing to perhaps reset it in the future in case the officer needs to be present (again).Ask a similar question
Thanks for the comment. I spoke with the clerk many times about my case. I just hired a lawyer. Lets see what happens.
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It's the "Cheer Melen Up Without Hurting His Back!" giveaway!
Please note: It is super important that you read and follow the directions.
M's in pain. Lots of pain. I gave a basic description of what's been going on here, if you're interested.
At the time of that writing, we'd decided to talk to a chiropractor about the situation before committing to surgery because M really didn't want surgery. And we did, but if we weren't optimistic before talking to the chiropractor, we were even less optimistic after our conversation (despite the fact that it would have been super cool to be able to say that M's doc used to work for the New York Giants). Not only because it kinda sounded like hooey, but also because M's insurance doesn't cover what the chiropractor wanted to do because the medical world isn't sure, yet, that it isn't hooey.
We had to go back to M's neurosurgeon to find that out. The chiropractor told us most insurances didn't cover it, but he didn't tell us that they didn't cover it because there's no confidence that it solves the problem.
So…we decided against the chiropractor's suggestion, and scheduled the surgery.
Once we'd made the decision, it all happened really fast. On Tuesday, we left the neurosurgeon, and drove to M's PCP for clearance. From there, we drove to the imaging place to pick up the disc containing M's MRI and X-rays. On Wednesday, we drove to St. Peter's Hospital for pre-op. On Thursday, my mom drove up from Maryland to drive us to and from the hospital (I still don't drive), keep me from completely losing my mind in the OR waiting room, and help us out for a couple days after the surgery. And Friday morning, we piled in the car at 8am and headed off to send M under the knife.
I was petrified. A lot of things can go wrong with spinal surgery. A LOT OF THINGS. And I couldn't stop thinking about each and every one of them.
I tried really hard not to let M know how scared I was. I sat with him while he waited to go back to the OR, and made jokes about the people on Let's Make a Deal, trying not to fidget. I have a permanent indentation on the inside of my cheek where I chewed relentlessly to avoid crying. And the second my mother and I were out of sight of M and the other people waiting for loved ones to come out of surgery, I completely fell apart.
I'm still worried. Who wouldn't be? But M made it through the surgery okay, and he's now in recovery. Slowly but surely, things are getting better, but Wednesday, he had a lot of swelling, and Thursday, he felt really sick.
This giveaway is all about new ways to cheer him up and keep him positive while he's healing. More about that in the entry directions. First, let's talk about those prizes!
The Prizes
First Prize: The New We-Vibe Touch provided by SheVibe.com!
Second Prize: The New Tantus Amsterdam Small provided by Tantus, Inc.!
Third Prize: The Lovehoney Beaded Sensual Glass Butt Plug provided by Lovehoney.com!
Honorable Mention: Gun Oil (silicone based, 2 oz), Gun Oil H2O (2 oz), Pink Water (3.3 oz), Pink Silicone (3.3 oz) provided by Pink and Gun Oil!
Social Media Prizes (2): A sample pack containing the following: Gun Oil High Caliber Male Performance Enhancement (dietary supplement), Gun Oil (silicone based), Gun Oil H2O, Gun Oil Force Recon (hybrid), Gun Oil Gel (water based), Gun Oil Loaded (water based cream), Gun Oil Stroke 29 (water based cream), Pink Elevate Female Performance Enhancement (dietary supplement), Pink Unity (hybrid), Pink Indulgence Crème, Pink Frolic (specialty toy lube), Hot Pink (warming lube), Pink Water, Pink Silicone, and a coupon for 30% off your next order at GetLube.com provided by Pink and Gun Oil!
Disclaimer: Use products at your own risk. Read all ingredients and instructions provided before use. Giveaway sponsors (SheVibe.com, Tantus, Lovehoney, Pink, Gun Oil) are not liable for any adverse reactions due to allergies or misuse of the products. Giveaway hosts (Melen and Rayne) are not liable for any adverse reactions due to use of these products.
How To Enter
There are a few ways to enter, the first being agreeing to the terms. That one is mandatory, and can be found in the widget below. Everyone must complete that entry to be considered, but that is the only entry that must be completed.
If you'd like to win First, Second, or Third Prize, or Honorable Mention, you must complete a task for me. It's super simple. Ready?
This thing with M has been going on since September of last year. I'm pretty good at cheering my man up, but I'm running out of ideas that won't hurt his back. My most recent attempt was buying smiley-faced french fries to have with dinner some night this week. Yeah…they're getting really lame. So! Your mission, should you choose to accept it (and if you don't, your only prize option is a sample pack of lubes and performance enhancement pills), is to throw out ideas for cheering M up and keeping him positive while he heals from spinal surgery. These things cannot involve sex (because cumming hurts…duh?), touching M's back, or too much bending or twisting on M's part. You can submit these entries via the comments below, or in an email to rayne(at)insatiabledesire[dot]com. Be sure to mark the "Cheer M Up!" entry option in the Rafflecopter widget below. You can make one suggestion each day of the contest.
The third and final way to enter is to share the shit out of this giveaway so other people have the chance to enter. Sharing is caring, after all. One winner will be chosen randomly via the Rafflecopter widget. The other will be whoever has the most social media entries. Each of the winners of these prizes will receive a giant sample pack of lube (listed above).


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Amateur video footage taken by a drone and uploaded to YouTube reveals the enormous scale of Apple's new "spaceship" headquarters now under construction in Silicon Valley, California.
The video shows a massive construction site, reinforced concrete foundations and what looks to be the gargantuan underground carpark that is designed to hide the eyesore of an outdoor parking lot.
The building, designed the British architectural firm Foster + Partners, is a four-storey, circular edifice built around a 60 hectare landscaped park. The complex will accommodate 12,000 employees.
The park at the core "would replicate the original California landscape, and parts of it would also recapture the orchards of [Silicon Valley's] past", Foster + Partners founder Sir Norman Foster told Architectural Record in an interview published earlier this year.
The late Apple cofounder, Steve Jobs, said the aim of the project was to build "the best office building in the world".
He was speaking shortly before his death in October, 2011 at a public meeting convened by the city council of Cupertino, where both Apple's existing headquarters and the new one are based.
Construction of the project, which Jobs said was inspired by the open spaces at the campus of nearby Stanford University, is not expected to be completed until 2016.


text:
Marquise Composite Diamond Accent Beaded Bridal Set in 10K Gold
Item# 19943760
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Why shop Peoples?
Sweet and sparkling, ask for her hand with this charming diamond-accented bridal set. Fashioned in warm 10K gold, the engagement ring features a marquise-shaped frame set with shimmering diamond accents and intricate beading. The gracefully twisting shank glistens with additional diamond accents. On your wedding day, the coordinating diamond-accented wedding band completes the ensemble. A meaningful gift of love, this bridal set is finished with a polished shine. View product details.
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text:
D5300 vs EOS 70D
KKhan wrote:
Please help me choose between the two cameras. I am new to DSLR and would love to learn more.
I already bought 70D few days ago. It takes stunning pictures. AF is amazing as long as you are using the view finder. WIFI for me has held good and I am able to freely move pictures to cell phones and post on facebook and whatsapp. I have been really wowed with this camera. I havenot used my flash at all with this camera. The only thing that bugs me is the weight. Almost 700 gms + 200-300 gms for lenses.
That brings me to Nikon 5300, everything almost similar. But the body is 500 gms almost 200 gms lighter than 70D.
I wanted to ask you guys, should I stick with 70 D where I know the pictures have been amazing and I would get used to extra 200 gms of weight in time or is D5300 same as 70D and worthwhile going down on the extra weight if that is bugging me. What are the big differences between the two cameras?
Here are the spec sheets for
70 d: http://www.dpreview.com/reviews/canon-eos-70d/2
5300: http://www.dpreview.com/products/nikon/slrs/nikon_d5300/specifications
Also: http://www.nikonusa.com/en/Nikon-Products/Product/Digital-SLR-Cameras/1519/D5300.html
I wish I could try d5300 in a store but cant find it in Portland, OR ( I m here till Sunday) before I go back to Kansas City.
200g really isn't that much, and you'll get used to it quickly. Mean while the 70D is a higher-level DSLR to the D5300, it's on par with the D7100 and shares a lot of the pro-level features like top LCD screen, two control dials and Auto Focus adjustments.
If you were really that concerned about weight, you'd be better off going mirrorless.
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Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats
Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools for treatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injured spinal cord still need to be improved. In the present study, we investigated whether
The spinal cords of adult Sprague-Dawley (SD) rats were completely transected at T10, five experimental groups were performed: 1. sham operated control (Sham-control); 2. operated control (Op-control); 3. electro-acupuncture treatment (EA); 4. MSCs transplantation (MSCs); and 5. MSCs transplantation combined with electro-acupuncture (MSCs+EA). After 2-8 weeks of MSCs transplantation plus EA treatment, we found that the neurotrophin-3 (NT-3), cAMP level, the differentiation of MSCs, the 5-HT positive and CGRP positive nerve fibers in the lesion site and nearby tissue of injured spinal cord were significantly increased in the MSCs+EA group as compared to the group of the MSCs transplantation or the EA treated alone. Furthermore, behavioral test and spinal cord evoked potentials detection demonstrated a significantly functional recovery in the MSCs +EA group.
These results suggest that EA treatment may promote grafted MSCs survival and differentiation; MSCs transplantation combined with EA treatment could promote axonal regeneration and partial locomotor functional recovery in the transected spinal cord in rats and indicate a promising avenue of treatment of spinal cord injury.
Functional deficits following spinal cord injury (SCI) result from neuronal damage, demyelination of axons, and dysfunctional neuroglial cells. The failure of axonal regeneration following SCI has been attributed to a non-permissive environment containing inflammatory mediators, lack of neurotrophic support, and inhibitory molecules. Numerous therapeutic strategies attempt to overcome these negative factors and promote axon regeneration, including transplanting tissue bridges
Previously, we reported that co-implanting Schwann cells and neural stem cells (NSCs) into a semi-transected or completely transected spinal cord significantly affected its recovery under certain circumstances
Acupuncture is a therapeutic technique used in traditional Chinese medicine. Since its development several thousand years ago, acupuncture has made many contributions to healthcare and medical treatment. Electro-acupuncture (EA) is a type of therapy in which a needle inserted into an acupoint is attached to a trace pulse current with the purpose of producing synthetic electric and needling stimulation. The application of EA for the treatment of SCI has shown promising results in the alleviation of the patients' suffering
Preparation of bone marrow mesenchymal stem cells (MSCs)
MSCs were prepared from one-month-old Sprague-Dawley (SD) rats in accordance with the procedure described in detail in our previous study
To verify the survival and transdifferentiation of transplanted MSCs, third or fourth generation MSCs were labeled with bromodeoxyuridine (BrdU, 1 μmol/L, Sigma) and Bisbenzimide (Hoechst33342, 10 μg/1 ml, Sigma). Two days before transplantation, MSCs were treated with 1 μmol/L BrdU for 48 h. In addition, MSCs were labeled with blue nuclear fluorescence Hoechst 33342 (10 μg/1 ml) 2 h before transplantation. Next, the MSCs were washed 3 times with D-Hank's balanced salt solution (HBSS, pH 7.4), and removed from the culture flasks using 0.25% trypsin/0.02% EDTA (Sigma). MSCs were centrifuged and resuspended in DMEM medium for cell counting. After undergoing the trypan blue dye exclusion test, the cell suspension was adjusted to a final concentration of 1×105 viable cells/μl in culture medium for transplantation.
Animal groups and spinal cord surgery
Experiments were performed on 66 adult female SD rats (Experimental Animal Center, Zhongshan School of Medicine of Sun Yat-sen University, China) weighing 230~250 g. Rats were housed in a temperature-controlled (24 ± 2°C) and light-controlled (12:12 light-dark cycle) room with free access to food and water. Prior to experimental manipulation, rats were allowed to acclimate to the housing facilities and were handled daily at least for 3 days. All experimental protocols and animal handling procedures were approved by the Animal Care and Use Committee of Sun Yat-sen University, and were consistent with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
The rats were divided into 5 groups. The first group, the sham-control group, received only a laminectomy (Sham-control). The remaining four groups underwent complete transections at the T10 spinal segment of the spinal cord as follows: the Op-control group received a spinal cord transection only; the EA group received EA treatment after the spinal cord transection; the MSCs group received MSCs transplantation after spinal cord transection; and the MSCs+EA group received transplanted MSCs with EA treatment after transection.
T9–T10 laminectomy: Briefly, the animals were anesthetized with 1% sodium pentobarbital (40 mg/kg, i.p.). A laminectomy was carried out at the T9-T10 level to expose the T10 spinal segment. The dura was cut and the T10 segment was transected completely with a scalpel with no tissue removed
Electro-acupuncture treatment
Two pairs of '
Figure 1
Schematic diagram indicates the four selected acupoints of the 'Governor vessel'
Schematic diagram indicates the four selected acupoints of the 'Governor vessel'. Changqiang (GV1), Yaoshu (GV2), Jizhong (GV6), and Zhiyang (GV9), which correspond to equivalent acupoints in humans.
Rats were loosely immobilized in a specially made restrainer that exposed their dorsal spine, hind legs, and tails. Two pairs of stainless silver needles of 0.3 mm in diameter were inserted into the GV1 and GV2, and GV6 and GV9, to a depth of 5 mm. The two pairs of needles were connected to the output terminals of an electro-acupuncture apparatus (Model G 6805-2, Shanghai Medical Electronic Apparatus Company, China). Alternating strings of dense-sparse frequencies (60 Hz for 1.05 s and 2 Hz for 2.85 s) were used. The intensity was adjusted to induce a slight twitch of the hindlimb (≤ 1 mA) and lasted for 20 min. EA was administered once every other day for 7 weeks, starting from the seventh day post-surgery.
cAMP and NT-3 assay
In accordance with our previous work
Previously, we tested the NT-3 level 2 weeks after the spinal cord transection and found the NT-3 level increased after EA treatment
Behavioral analysis
Both the BBB locomotor rating scale and inclined-grid climbing test were used to analyze the post-surgical motor behavior of the subjects' hindlimbs
Spinal cord evoked potentials (SCEP)
8 weeks after the spinal cord surgery, 23 rats (N = 5 in each group except sham control group = 3) were anesthetized with ketamine (40 mg/kg) and 1% sodium pentobarbital (30 mg/kg) and stereotaxically fixed. T1-L1 vertebrae were completely exposed. Briefly, The stimulation electrode was inserted into the T1-T2 interspinous ligaments, and a pair of needle electrodes was inserted into the interspinous ligaments of T12 to L1 for SCEP recording, according to a previous study
Immunohistochemical staining
Following the behavioral testing and evoked potentials recording, 23 rats of 5 groups were perfused by 4% paraformaldehyde in phosphate buffer (pH 7.4). Spinal cords were removed, post-fixed overnight in the paraformaldehyde, and cryoprotected in 0.1 M PB containing 30% sucrose at 4°C. Longitudinal cryosections (25 μm thickness) of spinal cord were cut and mounted on gelatin-coated slides for immunostaining. Primary antibodies were used as follows: mouse anti-oligodendrocyte special protein (clone CE-1, alternate name: MOSP, 1:1000, Chemicon); mouse anti-ED1 (a mouse monoclonal IgG, 1:200, Serotec, Indianapolis, IN); mouse anti-postsynaptic density (95 KDA) protein (PSD-95, 1:200, Sigma); rabbit polyclonal anti-serotonin (5-HT, 1:200, Sigma); rabbit polyclonal anti-calcitonin gene-related peptide (CGRP, 1:8000, Chemicon); rabbit polyclonal anti-glial fibrillary acidic protein (GFAP, 1:80, Sigma); rabbit polyclonal anti-synapsin I (1:500, Chemicon); rabbit polyclonal anti-neurofilament 150 KD (NF150, 1:400, Chemicon); rabbit polyclonal anti-β-tubulin III (1:100, Sigma). Cy3-conjugated goat anti-mouse IgG and Cy3-conjugated rabbit anti-goat IgG (1:800, Jackson Immunoresearch Labs, Inc.), FITC-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG (1:200, Jackson Immunoresearch Labs, Inc.) were used as secondary antibodies. Sections were washed three times with PBS and incubated with 10% normal goat serum with 0.3% Triton X-100 in PBS for 30 min at room temperature. Incubations with appropriate primary antibodies were performed overnight at 4°C. After repeated washing with PBS, sections were incubated with their respective secondary antibodies for 1 h at 37°C, washed with PBS, coverslipped, and examined under the fluorescence microscope.
Immunofluorescence double-labeling was performed to assess the survival and differentiation of transplanted MSCs. BrdU-labeled transplanted MSCs were identified by a mouse anti-BrdU antibody and visualized with FITC-conjugated goat secondary antibodies. Rabbit polyclonal anti-NF150 (for neurons) or rabbit polyclonal anti-β-tubulin III (for newly differentiated neurons) were used to identify the surviving MSCs that had differentiated into neurons.
A surviving cell count was conducted in 10 longitudinal spinal cord sections dissecting each animal's injury site, as a preceding study reported
Quantitative analysis of 5-HT and CGRP positive nerve fibers was performed according to prior studies
Additional file 1
A sketch of the injured spinal cord to clarify the location of quantification (rostral, caudal or the lesion site). The quantification of 5-HT positive fibers was performed at three regions (0.3 mm rostral to the transection site, the transection site and 0.3 mm caudal to the transection site) which were delineated by 4 red lines. The CGRP-positive fibers were quantified only in the lesion site (outlined by the two middle red lines) at 200 × magnification.
Click here for file
Data analysis
The total surviving MSCs and the percentages of differentiated cells were compared statistically between the MSCs+EA and MSCs groups using the Independent Samples T test. Other data were analyzed using one-way ANOVA or repeated-measure ANOVA. If equal variances were found, Fisher's least significant difference test was performed. Otherwise, the Kruskal-Wallis Test and Dunnett's T3 were used. The statistical significance level was set at P < 0.05.
cAMP and NT-3 level in transected spinal cord
Two weeks after the spinal cord surgery, the cAMP levels in the lesion site of spinal tissue were significantly increased in the MSCs+EA group compared to other groups (F = 49.32, df = 3, P = 0.000), but there was no significant difference between the Op-control group, EA group, and MSCs group (Fig.
Figure 2
cAMP and NT-3 level in the injured spinal cord
cAMP and NT-3 level in the injured spinal cord. A. cAMP levels in the lesion tissue were measured by radioimmunoassay. In the MSCs+EA group, cAMP levels in the lesioned tissue were significantly higher as compared to the other groups. *P < 0.001. Data = means ± SD. B. NT-3 contents in the segments rostrally and caudally to the lesion site, and the lesion site were measured by ELISA. In all three areas, the NT-3 contents were significantly increased in the EA, MSCs, and MSCs+EA groups as compared to the Op-control group (* indicates P < 0.01). In the lesion site, the NT-3 contents were significantly higher in the MSCs+EA group than the MSCs and EA groups (# indicates P < 0.01). Moreover, the NT-3 contents in the rostral and caudal segments to the lesion site in the MSCs+EA group had no significant difference as compared to the Sham-control group (P > 0.05). Data= means ± SD.
Four weeks following the spinal cord surgery, the NT-3 content at the lesion site in the MSCs+EA group was significantly increased compared to the MSCs or EA groups. However, NT-3 content at the rostral and caudal segments of the MSCs+EA group was not significantly different from that of the EA group. NT-3 in the Op-control group was the lowest (Fig.
Behavioral testing
After spinal cord transection, all the rats were paralyzed and moved by pulling themselves forward with their forelimbs. Moreover, they presented with urinary and fecal incontinence. The hindlimb locomotor activity of rats in each group was evaluated by the BBB open field test at five time points between 1 and 8 weeks post-lesion. Hindlimb locomotor activity improved gradually in the three treatment groups throughout the entire follow-up survival period. BBB scores gradually increased over time in each group. BBB scores were significantly higher in the three treatment groups compared to the Op-control group in the period from two weeks to eight weeks post-lesion (P < 0.05). However, MSCs+EA group showed a significant higher score compared with the EA and MSCs groups after the fourth week, most notably at the eighth week following the operation (P < 0.01) (Fig.
Figure 3
BBB open field test: BBB scores were obtained starting from the first week throughout the 8 week survival period (5 time points)
BBB open field test: BBB scores were obtained starting from the first week throughout the 8 week survival period (5 time points). The scores gradually increased with time in each group. BBB scores were significantly higher in the three treatment groups as compared to that of the Op-control group from two weeks to eight weeks post-transection (P < 0.05). The MSCs+EA group showed a significantly higher score than the other treatment groups beginning the 4th week following transection. Especially at eight weeks after the operation, the scores of the MSCs+EA group were significantly higher than those of the other groups (*indicates P < 0.01 as compared to the Op-contorl group; # indicates P < 0.01 compared to the EA group/MSCs group). Data = means ± SD.
An alternative function test, the inclined grid test examining limb coordination, was also performed. Rats in the MSCs+EA group voluntarily placed their paws on the grid and pushed themselves upwards using their hindlimbs to reach the horizontal platform. Rats in the MSCs and EA groups placed their paws on the grid occasionally. In contrast, rats in the SCI group struggled to climb onto the inclined grid with their forelimbs while their hindlimbs dragged passively behind their body and their hind paws often fell through the mesh. Thus, this test showed voluntary movement of the hindlimbs and plantar placement primarily in the MSCs+EA group.
Spinal cord evoked potentials (SCEP)
At the end of the eighth week following spinal cord injury, the SCEP in the Op-control group was weak, the latencies were prolonged, and the amplitudes were significantly decreased as compared with the other four groups (Fig.
Figure 4
Spinal cord evoked potential recovery after MSC transplantation and EA treatment
Spinal cord evoked potential recovery after MSC transplantation and EA treatment. A. The SCEPs of the rats in the Op-control group were very weak. After receiving EA, MSCs, or the combination therapy of EA and MSCs grafts, the SCEP of the rats recovered to different degrees. B. The restored activity of SCEP was abolished by a retransection, indicating descending axonal regeneration.
Figure 5
The latency and amplitude of SCEP at the end of 8 weeks after MSCs transplantation and EA treatment
The latency and amplitude of SCEP at the end of 8 weeks after MSCs transplantation and EA treatment. A. After spinal cord transection surgery, the latency of SCEP was significantly prolonged in Op-control group as compared with the Sham-control group (* indicates P < 0.05). However, the MSCs+EA group showed a significant recovery of the latency (# indicates P < 0.01, as compared to the Op-control group; $ indicates P < 0.05, as compared to the EA/MSCs group). B. After spinal cord transection surgery, the amplitude of SCEP was significantly declined in the Op-control group as compared to the Sham-control group (* indicates P < 0.05). However, in the MSCs, EA, or MSCs+EA group, the amplitude of SCEP was significantly recovered as compared with the Op-control group (# indicates P < 0.05). The MSCs+EA group showed a significant recovery of the amplitude as compared to the EA or MSCs group ($ indicates P < 0.05). Data = means ± SD.
Survival and differentiation of MSCs in transected spinal cord
During the 8th week after transplantation, the grafted cells labeled by BrdU could easily be identified with fluorescent microscopy, were mainly found in the lesion site, and were well integrated with the host tissue (Fig.
Figure 6
The survival and differentiation of MSCs in the injured spinal cord
The survival and differentiation of MSCs in the injured spinal cord. A. BrdU-labeled nuclei (green) of MSCs in the injured spinal cord in the MSCs+EA group. Grafted BrdU-labeled MSCs were well integrated with host tissue and migrated into host tissue over the host/lesion interface in the spinal cord both rostrally and caudally. Two broken lines outline the lesion site. Inset in A showing the BrdU-labeled MSCs – at a higher magnification (Scale bars: 80 μm; Insets, 20 μm.). B. The number of survived MSCs in the lesion site (MSCs+EA vs. MSCs, * indicates P < 0.05). Data = means ± SD. C. ED1-immunostained activated macrophages (red) in the lesion site; numerous grafted MSCs (Hoechst labeled the nuclei of MSCs, blue) survived in the lesion site, some were phagocytosed by ED1 immunoreactive activated macrophages (arrowheads). D. NF150-positive neuron-like cells (green, arrows) co-localized with Hoechst-labeled nuclei of MSCs (Hoechst, blue), but not with ED1-positive activated macrophages (red). Scale bars: A = 80 μm, C and D = 20 μm.
The longitudinal spinal cord sections were examined for differentiation markers for neuron, oligodendrocyte, and astrocyte lineages. Fluorescent immunohistochemistry staining revealed that transplanted MSCs were able to differentiate into either neuron-like cells or glial-like cells. We found that the majority of differentiated neural cells of the engrafted MSCs were localized in the host/lesion interfaces and the lesion site. The transplanted cells partially expressed the neuronal marker, NF150 (Fig.
Figure 7
Differentiation of MSCs into neuron-like and oligodendrocyte-like cells at 8 weeks following transplantation
Differentiation of MSCs into neuron-like and oligodendrocyte-like cells at 8 weeks following transplantation. A and B: NF150 (red) and BrdU (green) double-labeling show the NF150-positive neuron-like cells (arrows) in the MSCs group (A) and MSCs+EA (B) group. Cell number counting indicates the number of NF150/BrdU double-labeled cells was significantly higher in the MSCs+EA group compared to the MSCs group (C, * indicates P < 0.05). D and E: MOSP (red) and BrdU (green) double-labeling show the MOSP-positive oligodendrocyte-like cells (arrows) in the MSCs group (D) and MSCs+EA group (E). Cell number counting indicates the number of MOSP/BrdU double-labeled cells was significantly higher in the MSCs+EA group as compared to the MSCs group (F, * indicates P < 0.05). Data= means ± SD. Scale bars: A, B, D, E = 20 μm.
At early stages of the transplantation process, many grafted cells expressed nestin, a marker for neuroblast cells (Fig.
Additional file 2
BrdU and Hoechst co-labeling images in vitro and in vivo. A. The percentage of BrdU (red) and Hoechst (blue) co-nuclei (arrows) of MSCs was about 95% in vitro. B. Hoechst-labeled nuclei (blue, arrows) in the lesion site the MSCs+EA group. C. BrdU immunofluorescence showing BrdU-labeled nuclei (green, arrows) in the same field of A. D. image A and B showing BrdU and Hoechst co-nuclei of MSCs (blue-green, arrows). Scale bars: A = 40 μm, B, C, D = 20 μm.
Click here for file
Figure 8
Differentiation of MSCs into neuron-like cells at 8 weeks following transplantation
Differentiation of MSCs into neuron-like cells at 8 weeks following transplantation. A, B and C: Using different makers (nestin for neuroblast cells (A), β-tublin III for young neurons (B), and 5-HT for functional neurons (C) combined with a BrdU double-label), we found that MSCs transplanted into injured spinal cord tissue could survive and differentiate into neuronal phenotypes in the MSCs+EA group. D-F: In the MSCs group, a double immunofluorescence study of PSD95 (green) and synapsin I (red) showed a small number of coexpressing cells. G-I: Double immunofluorescence showed that some cells coexpressed PSD95 (green) and synapsin I (red) in the grafts of the EA+MSCs group. Scale bars: 20 μm.
5-HT and CGRP fibers regeneration
Serotonergic fibers originate in brain stem nuclei, especially in the raphe nuclei, and project throughout the longitudinal extent of the spinal cord. These fibers are thought to have a greater growth capacity following spinal cord injury and can be conveniently detected by 5-HT immunostaining
Figure 9
5-HT and CGRP-positive nerve fibers in the injured spinal cord at 8 weeks following surgery
5-HT and CGRP-positive nerve fibers in the injured spinal cord at 8 weeks following surgery. A-D: A longitudinal section of spinal cord shows 5-HT positive fibers in the Op-control group (A), EA group (B), MSCs group (C), and MSCs+EA group (D). a-d: Higher magnification of the rectangle boxes in Fig. A-D. Scale bars: A, B, C, D = 320 μm; a, b, c, d = 40 μm. E: Comparison of the number of 5-HT positive fibers in the lesion site and the region immediately rostral to the lesion site among the 4 groups (MSCs+EA vs. Op-control group, * indicates P < 0.01; MSCs+EA vs. MSCs or EA groups: # indicates P < 0.05). F-I: A longitudinal section of spinal cord shows CGRP-positive fibers (arrows) in the Op-control group (F), EA group (G), MSCs group (H), and MSCs+EA group (I). Scale bars: F, G, H, I = 20 μm. J:Comparison of the number of CGRP-positive fibers located in the lesion site in the 4 groups (MSCs+EA vs. Op-control group, *indicates P < 0.001; MSCs+EA vs. EA or MSCs groups, # indicates P < 0.001).
In the present study, we evaluated the effect of EA on the survival and differentiation of MSC transplantation, axonal regeneration, as well as functional recovery in rats with transected spinal cords. We found that the level of cAMP and NT-3, the number of vital and differentiated MSCs, the number of 5-HT-positive and CGRP-positive fibers in and near the lesion site of the injured spinal cord were all significantly increased in the MSCs+EA group as compared to the groups that underwent MSC transplantation or EA treatment alone. Furthermore, evidence from BBB scales and spinal cord evoked potentials demonstrated a significant functional recovery in the MSCs +EA group.
MSCs transplantation combined with EA treatment increased the cAMP level in the lesion site of the spinal cord, which is consistent with our previous study
In previous studies, we found that EA treatment increases the amount of NT-3 in the spinal cord tissue surrounding the lesion site 2 weeks after spinal cord transection
In this study, we found that the survival and differentiation of MSCs into neuron-like cells and oligodendrocytes were significantly increased in the MSCs+EA group, corresponding to a similar elevation in NT-3 and cAMP levels. This data suggests that EA promotes the survival and differentiation of grafted MSCs by elevating neurotrophic factors (such as NT-3) and the level of cAMP in injured spinal cord tissue. NT-3 is a significant member of the neurotrophic factors family and plays an important role during nervous system development, neuronal survival and differentiation, and neuronal repair via a signal transduction pathway
However, several observations have raised questions regarding neuronal transdifferentiation of MSCs and suggested that the transdifferentiation was attributed to cell fusion with host cells. Several laboratories have proposed that MSCs fuse with the host cells, including the neurons, and acquire their phenotypes, which simulate their transdifferentiation into host cells
We also found that 5-HT and CGRP-positive immunostained axons were regenerated in or across the lesion site into the caudal spinal cord at different degrees, most prominently in the MSCs combined with EA treatment group. It is known that scar formation by glia proliferation, lack of tropic support and inhibitory molecules
The detection and behavioral analysis of spinal cord evoked potentials suggest that MSC transplantation combined with EA treatment efficiently improves neuronal function recovery. MSCs transdifferentiate into neurons and oligodendrocytes to replace the damaged or dead neural cells or repair myelin sheaths in the injured spinal cord, and increase the number of 5-HT-fibers passing through the lesion site into the caudal spinal tissue, which may be the morphological basis of the functional outcomes. However, whether or not these "neural cells" neurons were in fact functionally replaced damaged spinal cord neurons needs to be confirmed.
In summary, our experiments have shown that MSC transplantation combined with EA treatment promotes MSC survival and differentiation into neural-like cells, stimulates axonal growth into the lesion/transplant site, and improves hindlimb locomotion. Increased NT-3 and cAMP levels in the injured/transplanted area may be the main molecular mechanism of the morphological and functional effects of the treatment. Our results suggest a new therapeutic potential of the combination of MSC transplantation with EA treatment to treat spinal cord injury patients.
Authors' contributions
YSZ designed the study, contributed to the experiments, and drafted the manuscript. YD designed the study, contributed to all experiments and analysis of the data, and drafted the manuscript. YQ participated in the design of the study, conducted the surgery and behavioral examination of rats and analyzed the subsequent data. JWR carried out the electro-acupunture treatment on rats. YQZ contributed to the cell cultures and performed the spinal cord transections in some rats. WJL and YJZ also performed some of the spinal cord transections. YL contributed to the creation of the antibody protocol. HXD contributed to the study design and general concept, and drafted the manuscript. All authors read, commented, and approved the final manuscript.
We thank Keely Murphy at Northwestern University for editing this manuscript. This research was supported by a grant from the Chinese National Natural Science Foundation (No. 30472132) and a Research Grant of the Administrative Bureau of Chinese Traditional Medicine of Guangdong Province (No. 1050167; 2007109) to Y.S. Zeng.
Spinal cord repair in adult paraplegic rats: partial restoration of hind limb function
Effects of an embryonic repair graft on recovery from spinal cord injury
Freeze-dried poly(D, L-lactic acid) macroporous guidance scaffolds impregnated with brain-derived neurotrophic factor in the transected adult rat thoracic spinal cord
Synthetic hydrogel guidance channels facilitate regeneration of adult rat brainstem motor axons after complete spinal cord transection
Induction of axon growth into Schwann cell implants grafted into lesioned adult rat spinal cord
Transplanted embryonic stem cells survive, differentiate and promote recovery in injured rat spinal cord
Features of skin-coincubated macrophages that promote recovery from spinal cord injury
Olfactory ensheathing cells genetically modified to secrete GDNF to promote spinal cord repair
Transplantation of bone marrow stromal cell-derived Schwann cells promotes axonal regeneration and functional recovery after complete transection of adult rat spinal cord
Transplantation of human neural stem cells for spinal cord injury in primates
A re-assessment of the consequences of delayed transplantation of olfactory lamina propria following complete spinal cord transection in rats
Treatment of the chronically injured spinal cord with neurotrophic factors can promote axonal regeneration from supraspinal neurons
Restriction of axonal retraction and promotion of axonal regeneration by chronically injured neurons after intraspinal treatment with glial cell line-derived neurotrophic factor (GDNF)
Glial cell line-derived neurotrophic factor-enriched bridging transplants promote propriospinal axonal regeneration and enhance myelination after spinal cord injury
Delayed transplantation of fibroblasts genetically modified to secrete BDNF and NT-3 into a spinal cord injury site is associated with limited recovery of function
cAMP and Schwann cells promote axonal growth and functional recovery after spinal cord injury
Combinatorial therapy with neurotrophins and cAMP promotes axonal regeneration beyond sites of spinal cord injury
Combining Schwann cell bridges and olfactory-ensheathing glia grafts with chondroitinase promotes locomotor recovery after complete transection of the spinal cord
Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord
Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury
Marrow stromal cells: implications in health and disease in the nervous system
Bone marrow-derived mesenchymal stromal cells for the repair of central nervous system injury
Expression of brain natriuretic peptide by human bone marrow stromal cells
Induction of neurotrophin expression via human adult mesenchymal stem cells: implication for cell therapy in neurodegenerative diseases
Protective effects of bone marrow stromal cell transplantation in injured rodent brain: synthesis of neurotrophic factors
Spinal cord injury in rat: treatment with bone marrow stromal cell transplantation
Adult rat and human bone marrow stromal cells differentiate into neurons
Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery
Adult bone marrow cells differentiate into neural phenotypes and improve functional recovery in rats following traumatic brain injury
Adult human bone marrow stromal spheres express neuronal traits in vitro and in a rat model of Parkinson's disease
Combination of denoviral vector-mediated neurotrophin-3 gene transfer and retinoic acid promotes adult bone marrow cells to differentiate into neuronal phenotypes
Remyelination of the spinal cord following intravenous delivery of bone marrow cells
Remyelination of the rat spinal cord by transplantation of identified bone marrow stromal cells
Clinical trial of acupuncture for patients with spinal cord injuries
Effect of Governer Meridian electro-acupuncture on water channel aquaporin-4 in experimental spinal cord injured rats
Effect of electroacupuncture at acupoints of the governor vessel on aquaporin-4 in rat with experimental spinal cord injury
Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats
Effects of Du Meridian electroacupuncture and transplanted neural stem cells on the functional recovery of the hind limbs in spinal cord transected rats
Combination of goremor vessels electroacupuncture and transplanted neural stem cells promotes injured spinal cord tissue producing nerve growth active substances
Functional recovery and regeneration of descending tracts in rats after spinal cord transection in infancy
Co-transplantation of neural stem cells and NT-3-overexpressing schwann cells in transected spinal cord
Cyclic AMP elevation is sufficient to promote the survival of spinal motor neurons in vitro
Sensitive and reliable locomotor rating scale for open field testing in rats
Prevention of spinal cord injury with time-frequency analysis of evoked potentials: an experimental study
BDNF-expressing marrow stromal cells support extensive axonal growth at sites of spinal cord injury
Orientation of neurite growth by extracellular electric fields
Perturbation of the direction of neurite growth by pulsed and focal electric fields
Adenylyl cyclases and the interaction between calcium and cAMP signaling
Requirement for BDNF in activity-dependent survival of cortical neurons
Multiple distinct signal pathways, including an autocrine neurotrophic mechanism, contribute to the survival-promoting effect of depolarization on spiral ganglion neurons in vitro
Neurotrophin-3 and Brain Derived neurotrophic factor induce oligodendrocytes proliferation and myelination of regenerating axons in the contused rat spinal cord
Intracerebral transplantation of marrow stromal cells cultured with neurotrophic factors promotes functional recovery in adult rats subjected to traumatic brain injury
Human bone marrow stromal cells cultures conditioned by traumatic brain tissue extracts: growth factor production
Expression of mRNAs for neurotrophic factors (NGF, BDNF, NT-3 and GDNF) and their receptors p75NGFR, trkA, trkB and trkC in the adult human peripheral nervous system and nonneural tissues
Possible role of CREB in the stimulation of oligodendrocyte precursor cell proliferation by neurotrophin-3
Neurotrophin-3 and a CREB-mediated signaling pathway regulate Bcl-2 expression in oligodendrocyte progenitor cells
Neurotrophin-3 specifically increases mature oligodendrocyte population and enhances remyelination after chemical demyelination of adult rat CNS
Differential effects of cAMP in neurons and astrocytes
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes
Differentiation, cell fusion, and nuclear fusion during ex vivo repair of epithelium by human adult stem cells from bone marrow stroma
Transplanted bone marrow regenerates liver by cell fusion
Bone marrow transdifferentiation in brain after transplantation: a retrospective study
Adult bone marrow stromal cells in the embryonic brain: engraftment, migration, differentiation, and long-term survival
Transplanted human bone marrow cells generate new brain cells
Bone marrow stromal cells protect and repair damaged neurons through multiple mechanisms
Glial inhibition of CNS axon regeneration
Effect of electroacupuncture on neurotrophin expression in cat spinal cord after partial dorsal rhizotomy
Changes of expression of glial cell line-derived neurotrophic factor and its receptor in dorsal root ganglions and spinal dorsal horn during electroacupuncture treatment in neuropathic pain rats
Treatment of neural injury with marrow stromal cells
Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms
Transplants of fibroblasts genetically modified to express BDNF promote regeneration of adult rat rubrospinal axons and recovery of forelimb function
Delayed grafting of BDNF and NT-3 producing fibroblasts into the injured spinal cord stimulates sprouting, partially rescues axotomized red nucleus neurons from loss and atrophy, and provides limited regeneration
Effect of electroacupuncture on proliferation of astrocytes after spinal cord injury


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Join as a member. There's a certificate with your name on it.
Last updated 23 March 2011. Created on 5 July 2010.
Edited by Mojah. Log in to edit this page.
Opengrid was developed by Mojah Media for the purpose of having an uncluttered and coherent interface through which various grid based layouts could be configured without having to delve into css or theme files. The theme serves as an ideal starting point and foundation upon which to design and test out different ways to present information.
Here's a short guide to get you going.
Pre-configuration Layouts Based on Active Sidebars Regions.
Opengrid has by default two lateral regions: first and second sidebar. These regions are visible only when there is content assigned to them, in that case it is said that the region is active.
Depending on which side regions are active in a given page, you may have four situations:
- neither sidebar is active
- both sidebars are active
- only the first sidebar is active
- only the second sidebar is active
Within the theme settings page can be seen the form that provides pre-configured the layout in each of these cases, allowing if necessary, to set different widths or positions in each of the regions, for each of the four situations listed above.
Grid Actions - How to set the grid.
When we use a grid-like framework, the width of our layout is divided into columns, the number of columns depends on the value chosen for the container, in our case the options will be 12 or 16 columns - with a fixed width for each column using the fixed container; or a fluid width using the liquid container.
Having defined the grid type, the layout is split in that number of columns, then you can begin to configure each region as a function of the grid.
Configuration options:
- Width: width of the region in number of columns
- prefix: padding left in columns
- Suffix: Padding right columns
- Push: Move right the region, in number of columns
- Pull: Move left the region, in number of columns
Considerations about Options Push & Pull.
The region where the main content is loaded is above the lateral regions, if we want to then show one of the sidebars in front of the region where the main content of the page is loaded, you should make use of push-pull options.
Suppose our design needs to show the first sidebar to the left of the main content, where also the dimensions in columns are:
- container: 16 columns
- sidebar first region: 5 columns
- Main content area: 10 columns
In this case we move:
- the region's main content to the right by an amount equal to the width of the first sidebar, ie Push: 5 columns
- the sidebar to the left first by an amount equal to the width of main content region, ie 10 columns Pull
About Opengrid Layout - Containers & Regions
Opengrid is a theme whose structure is built using a system of grids. One of the major limitations that can be found from the design point of view of this type of framewprk, is the fixed width. Some designs need backgrounds that span the entire width of the screen, where clearly a fixed width would make it impossible to use a theme based on grids.
To circumvent this problem Opengrid implements a system of independent containers, thus each region is surrounded by a div container, said container in turn is surrounded by a div, the latter div being independent of the grid system, allowing for a background that can be applied to the entire width of the screen, thus removing the limitation mentioned above. Where not required for these backgrounds can be used as general container the div # page.
Extra Classes - Adding Personal Classes
Perhaps the most difficult concept to understand when you start working with a grid-like system, is the investment in the workflow of labor between HTML and CSS. Conventionally, at least for most people, the first step is to set out the structure of the HTML page, defining id's and classes to be later styled by css. By working with grids this process is reversed, thus we have a CSS file with existing classes that we apply to our HTML layout in order to build the site.
We define and load classes (now converted into variables) via the theme settings page that are applied to the html generated by Drupal and the theme engine. This methodology opens up a range of options regarding how to apply styles to our theme. In the same way we apply classes/variables related to the grid system, we can create new style sheets with pre-defined classes that may be applied to each region.
Example:Styles for lists by region.
Let's define a class style sheet with styles that apply to lists.
.Red-navigation ul li a (
color: red;
. Blue-navigation ul li a (
color: blue;
. Green-navigation ul li a (
color: green;
now, from the theme settings form, look for the section of "both sidebars active" and for the regions main , first and second sidebar assign each one a different class from which we just created,
- main -> extra class: red-navigation
- first sidebar -> extra class: blue-navigation
- second sidebar -> extra class: green-navigation
For each of the regions will have lists of links of different colors.
The example, although simple, gives an idea of what is possible to achieve with pre-configured css classes. In terms of reusable css, it is very interesting.
Drupal starter theme
Drupal foundation theme
Drupal base theme
Drupal grid base theme
Looking for support? Visit the Drupal.org forums, or join #drupal-support in IRC.


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Harris Home Furnishings is a furniture retailer that sells mattresses in addition to other types of home ...
→ Visit the Harris Home Furnishings profile
Harris Home Furnishings in Eatonton, GA carries the following products. Select a manufacturer below to view the lines and collections carried by this store:
Sealy produces a broad range of innerspring and specialty mattresses and foundations through the Sealy, Sealy Posturepedic and Optimum brands ... See Sealy models »
Have you shopped for a mattress at Harris Home Furnishings? Write a Review!
Harris Home Furnishings is a furniture retailer that sells mattresses in addition to other types of home furnishings. Harris Home Furnishings carries a variety of mattress models from which to choose, including mattresses from Restonic and Sealy. Harris Home Furnishings has furniture stores in Georgia.
View the Harris Home Furnishings profile →
Harris Home Furnishings is a general furniture store in Eatonton, GA. As a furniture retailer featuring a broad selection of mattresses, Harris Home Furnishings carries mattress models from Sealy.
Have an update or correction to our information about Harris Home Furnishings? If so, please take a moment to suggest an edit.
If this is your business, claim your profile to update this information directly. (What's this?)
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Get started on GoodBed by claiming your business profile. Companies in the GoodBed community believe in providing consumers with straightforward information, quality products, and good service. If you share these beliefs, join us by claiming your free profile today.
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Islamabad, Dec 9 (IANS) President Asif Ali Zardari, who left Pakistan Tuesday after suffering a stroke that caused bleeding in the brain and facial paralysis, will continue to be in Dubai for over two weeks and may even go to London for further treatment, said media reports.
Zardari abruptly left Pakistan for Dubai Tuesday, triggering speculation about his health. There were also rumours of a military coup, which the US scotched Thursday.
Latest media reports said Asif Ali Zardari had suffered a mild stroke as the president's aides insisted his health was improving and he was 'stable, comfortable and resting' in a Dubai hospital.
The mild stroke caused internal bleeding in the brain and facial paralysis, the News International quoted sources as saying.
Sources added that though medical test reports were clear and he was out of danger as the stroke and internal bleeding were not life threatening, the president would need long period of treatment, including speech therapy, to resume his normal life.
Britain's Telegraph said Dubai doctors have diagnosed the ailment as transient ischemic attack. It is caused by a blockage that prevents blood flow to a part of the brain and leads to stroke-like symptoms.
Zardari will continue to be under medical observation at the American Hospital in Dubai and the period may even be more than two weeks before he returns home, Gulf News reported.
The president was shifted out from an ICU to a hospital room Thursday evening.
Zardari's office said his health is improving and he is resting at the Dubai hospital.
The Aiwan-e-Sadr, the president's residence and principal workplace, issued a statement Thursday that said: 'The president is stable, comfortable and is resting.'
'Initial tests and investigations have been within normal range while further tests will be carried out,' the brief statement said.
However, a source quoted by the News International said Zardari had collapsed in the presidency Tuesday due to the stroke and may be taken to London for further treatment.
Zardari is high blood pressure patient and takes blood thinners.
There have been conflicting reports about Zardari's health.
The official line, a day after Zardari left Pakistan, was that he had gone to Dubai for medical tests and also to meet his children, but army doctors who carried out a medical check declared him fit.
Doctors from the Armed Forces Institute of Cardiology (AFIC) had found Zardari fit after a check-up.
On the other hand, the US-based Foreign Policy magazine quoted a former American official as saying that sections of the US government were informed that Zardari had a 'minor heart attack' Monday night. He had flown to Dubai via an air ambulance. Zardari may have to undergo an angioplasty procedure Wednesday and may also resign on account of 'ill health', the media report said.
The president's sudden departure had led to coup rumours.
Interior Minister Rehman Malik Friday tried to douse those rumours by saying: 'We understand that the people have given us a mandate for five years through elections and votes, and if someone tried to do something to the government, people would foil such attempts.'


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Microwave Mini Taiyaki Maker Set
Taiyaki is a hot pancake type of Japanese street snack that usually comes with red bean filling. These are made inside molds that resemble waffle molds and are usually cooked over fire or electric stoves.
Unlike traditionally made taiyaki, now with this set of taiyaki making tools one can make tasty taiyaki at home using just a microwave!
All it takes is to prepare the ingredients such as pancake mix, milk, eggs, mix them in the shaker provided and fill the mold with the batter and filling. After which it goes into the microwave for approximately 1 and half minutes and a delicious Japanese snack is ready to be eaten.
Depending on one's skill, it may or may not come close in taste as those bought from the streets but the convenience of being able to eat hot and piping taiyaki anytime of the day is surely a big advantage, and it's safe for kids to make it for themselves too. The microwave mini taiyaki maker set is available for 3,500 yen at Strapya-World.


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Something you might not know about me – I'm a little bit Irish – so I love St. Patrick's Day 🙂 It's fun! Soooo I had to take some of the minty M&M's I had in the cabinet & make one of my most favorite quick snacks. Minty Shamrock M&M Popcorn is so easy to make & my kids went nuts for it with that hint of mint. Gotta love M&M's for keeping every holiday fun with all their different flavors & varieties.
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Federal spending cuts have limited grants for Bell County and Copperas Cove that would help supply new state-mandated radio equipment.
"We came up with a plan to change everyone over in our region on a five-year plan," said Jim Reed, executive director of the Central Texas Council of Governments, which helps manage several regional issues for multiple counties.
Funding for the emergency response radio system was allocated from Federal Emergency Management Agency grants the council of governments expected to regularly receive, Reed said.
"But (the funding) was cut by the federal government," he said.
Copperas Cove Deputy Fire Chief Gary Young said the new system will allow every emergency responder in Texas to communicate with each other.
Each year, the FEMA grant that was supposed to supply those funds decreased, Reed said. Now, the region's five-year plan looks more like a 10-year plan.
Copperas Cove and Bell County were to receive funds during a four-year period. During the first year, all the money was given to rural areas, he said. Bell County and Copperas Cove agreed to an 80/20 percent split on funds.
This year, Copperas Cove got about $60,000, Young said. The city will use the money either to purchase some of the remaining radios or on infrastructure, he said.
During the last three years, Copperas Cove received about $240,000 for the radios, with $80,000 last year, Young said.
The grants helped to purchase 76 of 345 communications systems needed in the city at a cost of about $3,300 each.
The intention was that the five-year plan would fund all the radios, Young said, "but the funding in the five-year plan was (originally) for much more than we have been getting."
To prevent residents from funding the radios, Copperas Cove has been vigilant in trying to obtain other grants.
Earlier this month, Copperas Cove received a grant for about $100,000. It had a 10 percent match, however, which means the city will chip in $10,000 for the radio purchase.
Young said the money should supply nearly 30 radios for the Cove fire department.
Reed said he was sure Bell County was attempting to take the same steps to fund radio and infrastructure upgrades to be in compliance with state law.
Reed estimated there are between 1,000 and 2,000 radios servicing Bell County, some of which have already been updated.


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Tags: signs, street signs
Where I live, street signs often have a little bit of local history written below.
东风西路 runs north south; it is further broken up into 东风西路北 and 东风西路南。Further east 东风东路 also runs north south; it is also broken up into 东风东路北 and 东风东路南. Helpfully, the signs also give distances to the next major intersection or landmark.
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Benjamin Franklin Born on
Janurary 17, 1706
Boston, Massachusetts Other Important Events In His Life Benjamin Franklin was many things ,but he was best known for
being one of the founding Fathers,
an American politician, an
inventor, and one of the signers
of the US Constitution.
He was also a magnificent scientist,
printer, writer, statesman, diplomat,
and a absolute civic leader. After a long and challenging life, he dies a peaceful
deaf on April 17,1790 in Philadelphia, PA. Franklin's Deaf Leadership Qualities
great nagotiator
confident public speaker
contributed to his community Franklin deserves a perfect ten because he was a true genius and an inspiring politician. He gave out the idea of the three branches of government that help maintain the U.S today. He convinced France to fight along with the Americans against the British. His impressive resourceful intelligence helped us win the Revolution and save many lives. All his dedicated work to crafting the United States paid off. Perfect 10 He discovered
by using a
key and a kite. In 1730, he
Read Rogers Sources
"Benjamin Franklin." Science and Its Times. Ed. Neil Schlager and Josh Lauer. Vol. 4. Detroit: Gale, 2001. Gale Biography In Context. Web. 17 May 2011.
"Benjamin Franklin." Encyclopedia of Occultism and Parapsychology. Detroit: Gale, 2001. Gale Biography In Context. Web. 17 May 2011.
"Benjamin Franklin 1706-1790." American Eras. Detroit: Gale, 1997. Gale Biography In Context. Web. 17 May 2011. Adminster to France
in 1779
and also served as
ambassador to Britain Signed the U.S Constitution in September 17,1787. Failures:
Any Successes:
invented useful inventions
convinced France to fight along
side with America
helped stop slavery
helped establish a better
discovered electricity Created by Jessoca Mendoza
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Benjamin Franklin: Why He Should Be Remembered.
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jessica mendozaon 17 May 2011
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