text:
Genome-wide analysis of small RNAs reveals eight fiber elongation-related and 257 novel microRNAs in elongating cotton fiber cells
MicroRNAs (miRNAs) and other types of small regulatory RNAs play critical roles in the regulation of gene expression at the post-transcriptional level in plants. Cotton is one of the most economically important crops, but little is known about the roles of miRNAs during cotton fiber elongation.
Here, we combined high-throughput sequencing with computational analysis to identify small RNAs (sRNAs) related to cotton fiber elongation in
This study not only facilitated the discovery of 257 novel low-abundance miRNAs in elongating cotton fiber cells but also revealed a potential regulatory network of nine sRNAs important for fiber elongation. The identification and characterization of miRNAs in elongating cotton fiber cells might promote the further study of fiber miRNA regulation mechanisms and provide insight into the importance of miRNAs in cotton.
Cotton (
Small RNAs (sRNAs), which range in size from 18 to 24 nt, can regulate gene expression at the transcriptional and/or post-transcriptional level
A total of 31 miRNA families, including 27 conserved and four novel miRNA families, have been identified in developing cotton ovules using a high-throughput sequencing approach, which showed that the general repression of miRNAs in fibers is associated with the upregulation of a dozen validated miRNA targets that are highly expressed in cotton fibers
In the present study, we performed high-throughput sequencing of cotton fiber sRNAs to identify novel and potential fiber elongation-related miRNAs. Four small RNA libraries were constructed from cotton fibers sampled at four different time points from 5 to 20 dpa, and more than 18 million short RNA sequence reads were generated for each library. The comparative miRNAomic analysis revealed 257 novel miRNAs and nine fiber elongation-associated sRNAs, including eight miRNAs and one tasiRNA, in allotetraploid cotton fiber cells. The predicted targets of the nine sRNAs were experimentally validated and implicated in different cellular responses and metabolic processes, demonstrating the regulatory importance of miRNAs and tasiRNAs in cotton fiber elongation. Thus, this study advances our understanding of the important regulation of miRNAs and tasiRNAs in allotetraploid cotton through the identification of new miRNAs and a putative miRNA-mediated regulatory network supporting cotton fiber cell elongation.
Results and discussion
High-throughput sequencing data overview
To identify new miRNAs and potential fiber elongation-associated miRNAs, four small RNA libraries were generated from allotetraploid cotton fibers at 5, 10, 15 and 20 dpa and then subjected to Illumina high-throughput sequencing. The obtained raw sequence reads (a total of 76.2 million reads from four fiber libraries) were computationally processed to remove low-quality reads, contaminated adapters and reads shorter than 18 nt, yielding more than 74.2 million clean reads (97.4% of total raw reads); 21–24 nt sequences accounted for 84.3% of the total clean reads (Figure
Figure 1
Sequence length distribution of the clean reads from cotton fiber small RNA libraries isolated at 5, 10, 15 and 20 dpa
Sequence length distribution of the clean reads from cotton fiber small RNA libraries isolated at 5, 10, 15 and 20 dpa. The 24 nt group is clearly much larger than the others.
The clean reads were mapped to the diploid cotton
Known miRNAs in elongating cotton fiber cells
Currently, miRBase (version 19.0, released on August 1, 2012) lists just 43 mature miRNA genes belonging to 21 miRNA gene families as cloned or predicted to be expressed in cotton. Based on our previous work, 36 new miRNAs from cotton ovules were officially named by miRBase and included in the subsequent release
Additional file 1: Table S1
The 79 known cotton miRNA families expressed in cotton fibers.
Click here for file
To accurately measure miRNA read abundances, the abundances of known miRNAs were normalized to reads per ten million (RPTM). The four miRNA families with the greatest abundance were 167 (164706 RPTM), 156/157 (86430 RPTM), 165/166 (71375 RPTM), and 894 (39985 RPTM), accounting for 41.3%, 21.7%, 17.9% and 10.0% of all the known miRNA reads, respectively. The miRNA families 164, 168, 3476, 7508, 172, 390, 535, 159/319, 2911, 2950 and 396-3p also exhibited average abundances of more than 1000 RPTM. In contrast, certain miRNA families were expressed at low abundance (Additional file
A total of 213 potential miRNA gene loci, belonging to 48 miRNA families, were identified based on the EST sequences (CGI11), genome survey sequences (GSS) and
Additional file 2: Table S2
The 213 known cotton miRNA precursors derived from ESTs (CGI11), genome survey sequences (GSS) and G. raimondii genome sequences.
Click here for file
Additional file 3: Figure S1
The 139 novel stem-loop structures of known miRNA precursors in elongating cotton fibers. In most cases, the red highlighting indicates the miRNA sequence and the blue highlighting indicates the miRNA* sequence. For example, in '17-MIR159-[hbr-MIR159a MI0022053]', the '17' came from the "index" column in the Additional file
Click here for file
Of the 48 known families with precursors, 26 had miRNA* sequences, providing evidence for a DCL1-processed stem-loop (Additional file
The sequence of the most abundant miRNA in each miRNA family (i.e. the representative miRNA sequence) is shown in the "Sequence" column of Additional file
Newly identified miRNAs in elongating cotton fibers
As shown in the analysis of known miRNA precursors, 174 of 213 precursors originated from
Additional file 4: Table S3
The 257 novel miRNAs in cotton fibers.
Click here for file
Additional file 5: Figure S2
The 314 Vienna RNA secondary structures of 257 novel miRNAs. The Vienna structures showed that the reliable candidate miRNA reads (at least five reads) accounted for more than 75% of the corresponding sets of reads according to rule (1) described in the "newly identified miRNAs in elongating cotton fibers" section.
Click here for file
Additional file 6: Figure S3
The 310 stem-loop structures of novel miRNA precursors in cotton fibers. The stem-loop structure shows the miRNA* sequence, mismatches between the miRNA and the other arm of the hairpin, and the size and frequency of asymmetric bulges. There were 314 novel miRNA gene loci, as shown in Additional file
Click here for file
Additional file 7: Figure S4
Genomic overview of 174 known and 311 novel GhmiRNA gene loci on the 13
Click here for file
Cotton fiber elongation-related miRNAs
To elucidate the potential regulatory roles of miRNAs in cotton fiber elongation, we analyzed the differential expression profiles of miRNAs. To minimize noise and improve accuracy, only miRNAs expressed at greater than 100 RPTM in at least one library were used for comparison. After Fisher's test (p < 0.01) and cluster analyses, as shown in Figure
Figure 2
The 46 cotton fiber elongation-related miRNAs
The 46 cotton fiber elongation-related miRNAs. (A) Complete linkage hierarchical cluster analysis of differential expression miRNAs in the cotton fibers (5–20 dpa) was performed by comparing the RPTM of GhmiRNA in every library to the average of the four cotton fiber sRNA libraries. The color indicates the fold-change as log2, from high (red) to low (green), as indicated in the color scale. On the right are names of the GhmiRNAs, on the left are the types to which they belong, and on the top is the dendrogram of the 5, 10, 15 and 20 dpa small RNA libraries generated by the cluster analysis of the 46 differentially expressed miRNAs. (B) The average abundances of 46 cotton fiber elongation-related miRNAs. RPTM, reads per ten million. Types A, B and C are shown in red, yellow, and blue, respectively, in Figure
Additional file 8: Figure S5
Quantitative RT-PCR analysis of GhmiR156, GhmiR167 and GhmiR168. The data represent the mean values ± SD of three replicates.
Click here for file
A newly identified tasiRNA in cotton
tasiRNA is an important clade of regulatory sRNAs in plants, although they have not been previously identified in cotton. In Arabidopsis, four canonical
Figure 3
miRNA-derived tasiRNA in
miRNA-derived tasiRNA in
Similar to the gene structure in Arabidopsis, the tasiRNA-generating locus in
Additional file 9: Figure S6
Size distribution of cotton ta-siRNA generated from the DW503626 gene.
Click here for file
We analyzed the expression patterns of DW503626 5'D7 (+), considered a homolog of functional tasiRNAs in Arabidopsis, during fiber development based on the sequence number. The accumulation of tasiRNA increased from 5 to 10 dpa and decreased from 10 to 20 dpa (Figure
tasiR-ARF, a conserved tasiRNA that targets ARFs in plant species, was required for proper leaf polarity development
Target prediction, validation, and expression correlated with miRNAs
To characterize the functions of miRNAs with differential RPTM in cotton fiber elongation, we first predicted and experimentally validated their targets. Using the psRNATarget Web server (
Additional file 10: Table S4
Target prediction for novel cotton fiber elongation-related miRNAs.
Click here for file
Additional file 11: Table S5
Target prediction for known cotton fiber elongation-related miRNAs.
Click here for file
Using this strategy, miRNA-guided target cleavage was examined for targets of the miRNAs listed in Figure
Figure 4
Target gene validation and expression analysis
Target gene validation and expression analysis. (A) Mapping of target mRNA cleavage sites using 5'-RLM-RACE. The arrows indicate the cleavage sites and the number shows the frequency of the clones sequenced. (B) Quantitative real-time PCR analysis of the target mRNAs. The data represent the mean values ± SD of three replicates. The dots represent the corresponding abundance on the right axis in four small RNA libraries. TC241907: ARF8 (auxin response factor 8); TC236756: MYB; TC259543: CaMBP (calmodulin-binding protein); TC229767: ACCD (acetyl-CoA carboxylase beta subunit); TC251689: CIBP (calcium ion-binding protein); TC246633: LRR-RLK (leucine-rich repeat receptor-like protein kinase); TC277934: SPL9 (squamosa promoter-binding protein-like 9); TC279526: GHHB8 (HD-Zip protein 8).
As shown in Figure
The expression of validated miRNA target genes was examined using qRT–PCR to determine whether there was a negative correlation between the accumulation of miRNAs and their targets at various periods during development. As shown in Figure
A potential functional network of fiber elongation-related tasiRNA and miRNAs
The unique biological process of cotton fiber elongation relies on the complex regulation of gene expression. miRNAs, which are small but crucial molecules in the regulation of gene expression, play an important role in the process of fiber elongation
Figure 5
A potential functional network mediated through miRNAs during fiber elongation
A potential functional network mediated through miRNAs during fiber elongation. The color of the miRNA box indicates the type of expression pattern and is consistent with the data shown in Figure
For a cotton fiber cell, the precise control of the elongation period is critical. It is not known how the fiber cell knows when to start or end the rapid elongation stage. However, an H2O2 burst at 20 dpa may serve as a termination signal for rapid fiber elongation
Additional file 12: Figure S7
GhSPL9, a target of GhmiR156/157, may positively regulate anthocyanin synthesis in cotton fiber. (A) Quantitative RT-PCR analysis of
Click here for file
Calcium-mediated signal transduction plays crucial roles in a wide array of growth and developmental processes. In plants, changes in cellular Ca2+ concentration are transmitted through several calcium sensors or calmodulin (CaM)
Various studies on cotton fiber cell development have identified plant hormones as critical regulators of fiber development. Auxin promotes the fiber cell development of
Additional file 13: Figure S8
Quantitative RT-PCR analysis of
Click here for file
The rapid elongation of cotton fiber cells requires substantial lipid synthesis to support the developing organelles and membranes. ACCD, the target of GhmiRnE, encodes the β-carboxyl transferase subunit of acetyl-CoA carboxylase (ACCase, EC 188.8.131.52), which is a regulatory enzyme in fatty acid synthesis
Additional file 14: Table S6
Normalized abundances of miRNAs and tasiRNA in fibers and seeds at 15 dpa.
Click here for file
In this study, 46 GhmiRNAs were differentially expressed during the cotton fiber elongation progress, 40 (Type of A and B GhmiRNAs) of which were dramatically upregulated during rapid fiber cell elongation (10 and 15 dpa). In Arabidopsis, miRNAs are loaded into AGO1, which acts an RNA slicer and is the target of miR168
Furthermore, the abundance of GhmiRNAs in this network accounted for approximately 80% of the total miRNA reads. As shown in Additional file
A total of 79 known miRNA families and 257 novel miRNAs from cotton fibers were identified through the high-throughput sequencing of sRNAs isolated from 5, 10, 15 and 20 dpa cotton fibers. This expands the population of known
Plant materials
Upland cotton (
Small RNA library construction and Solexa sequencing
Total RNA was extracted from the frozen 5, 10, 15 and 20 dpa fibers and the 15 dpa young seeds (as a reference) using the PureLink™ Plant RNA Reagent kit (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. The RNA quality was examined on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) (Additional file
Additional file 15: Figure S9
RNA integrity of samples from 5, 10, 15 and 20 dpa fibers and 15 dpa seeds. Right: Electropherograms of the samples, as generated by the Agilent 2100 Bioanalyzer. The x-axis indicates the size of the nucleic acid, and the y-axis indicates the fluorescence. The red and black arrowheads indicate the 28S and 18S ribosomal RNA peaks, respectively. The RIN and 28S to 18S ratio are also shown in the image. RIN: RNA integrity number; FU: Fluorescence units.
Click here for file
Analysis of sequencing data
Sequences were trimmed to eliminate low-quality reads and other contaminants from the adaptor tags. Repeats and protein coding sequences derived small RNAs were identified using a BLAST search against the annotated
Identification of elongation-related miRNAs and target prediction
The abundances of known and novel miRNAs in each library were reported as normalized reads (reads per ten million, RPTM). The miRNA families with reads greater than 100 RPTM in at least one library were subjected to Student's
The psRNATarget web server (
5'-RACE and quantitative RT-PCR
To validate the predicted cotton miRNA target genes
Additional file 16: Table S7
The primers used in this study.
Click here for file
Anthocyanin measurement
A previously described method
miRNAs: MicroRNAs; ARF: Auxin response factor; siRNA: Small interfering RNA; rasiRNAs: Repeat-associated siRNAs; tasiRNAs or TAS:
Competing interests
The authors declare no competing financial interests.
Authors' contributions
JYL conceived and designed the study. WX prepared the RNA and analyzed the sequencing data. ZW performed the tasiRNA analysis. XW and ZW performed qRT-PCR. ZW performed the anthocyanin measurement. WX, ZW, MD and YL performed the 5' RACE. JYL, WX and ZW wrote the manuscript. All authors read and approved the final manuscript.
The authors would like to thank Yu Zhang for the bioinformatics analysis. This work was supported by grants from the State Key Basic Research and Development Plan (2010CB126003) and the National Transgenic Animals and Plants Research Project (2011ZX08005-003 and 2011ZX08009-003).
Development of the cotton fiber
Comparative proteomic analysis provides new insights into the fiber elongating process in cotton
Metabolic pathway engineering in cotton: biosynthesis of polyhydroxybutyrate in fiber cells
Cotton fiber growth in planta and in vitro. Models for plant cell elongation and cell wall biogenesis
The control of single-celled cotton fiber elongation by developmentally reversible gating of plasmodesmata and coordinated expression of sucrose and K+ transporters and expansin
Developmental and molecular physiological evidence for the role of phosphoenolpyruvate carboxylase in rapid cotton fibre elongation
Unraveling mechanisms of cell expansion linking solute transport, metabolism, plasmodesmtal gating and cell wall dynamics
The cotton kinesin-like calmodulin-binding protein associates with cortical microtubules in cotton fibers
Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development
The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers
Molecular mechanisms of fiber differential development between
Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis
Small RNAs and their roles in plant development
A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development
MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes
The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis
A plant miRNA contributes to antibacterial resistance by repressing auxin signaling
Genome-wide analysis reveals rapid and dynamic changes in miRNA and siRNA sequence and expression during ovule and fiber development in allotetraploid cotton (
Enrichment of a set of microRNAs during the cotton fiber development
A Comparative miRNAome Analysis Reveals Seven Fiber Initiation-Related and 36 Novel miRNAs in Developing Cotton Ovules
MicroRNAs and their diverse functions in plants
The draft genome of a diploid cotton
Repeated polyploidization of
Conservation and divergence in plant microRNAs
Small RNA profiling in two Brassica napus cultivars identifies microRNAs with oil production- and development-correlated expression and new small RNA classes
Adenylation of plant miRNAs
Criteria for annotation of plant MicroRNAs
DNA methylation mediated by a microRNA pathway
Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide
microRNA-directed phasing during trans-acting siRNA biogenesis in plants
DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7
Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Regulation of AUXIN RESPONSE FACTOR3 by TAS3 ta-siRNA affects developmental timing and patterning in Arabidopsis
AtXRN4 degrades mRNA in Arabidopsis and its substrates include selected miRNA targets
Control of leaf morphogenesis by microRNAs
Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology
Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in Arabidopsis
Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor
Plant-specific calmodulin-binding proteins
Genes encoding calmodulin-binding proteins in the Arabidopsis genome
Physiological and biochemical changes associated with cotton fiber development: IX. Role of IAA and PAA
Auxin response factors
'Evidence of an auxin signal pathway, microRNA167-ARF8-GH3, and its response to exogenous auxin in cultured rice cells'
Disruption and overexpression of auxin response factor 8 gene of Arabidopsis affect hypocotyl elongation and root growth habit, indicating its possible involvement in auxin homeostasis in light condition
Cell-specific nitrogen responses mediate developmental plasticity
Characterization of the brassinosteroid insensitive 1 genes of cotton
Plant acetyl-CoA carboxylase: structure, biosynthesis, regulation, and gene manipulation for plant breeding
Dissecting functions of KATANIN and WRINKLED1 in cotton fiber development by virus-induced gene silencing
Genomics of cotton fiber secondary wall deposition and cellulose biogenesis
Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis
The arabidopsis ATHB-8 HD-zip protein acts as a differentiation-promoting transcription factor of the vascular meristems
Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate
Characterization of microRNAs expressed during secondary wall biosynthesis in Acacia mangium
The genetic control of lignin deposition during plant growth and development
The eli1 mutation reveals a link between cell expansion and secondary cell wall formation in Arabidopsis thaliana
The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development
Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs
Molecular phylogeny of miR390-guided trans-acting siRNA genes (
Statistical analysis of a small set of time-ordered gene expression data using linear splines
GhMYB25-like: a key factor in early cotton fibre development
Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
Molecular mechanism for jasmonate-induction of anthocyanin accumulation in Arabidopsis


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29 October 2008,
Beverly Hills
Price realised USD 47,500
Guy Rose (1867-1925)
Still Life with Onions, Peppers and Basket
indistinctly signed, dated and inscribed 'Guy Rose/87/To Annie & Ned/-July88-' (upper right)
oil on canvas
20 x 26 in. (50.8 x 66 cm.)
New York +1 212 636 2000
London +44 (0)20 7839 9060
Hong Kong +852 2760 1766
Shanghai +86 21 6355 1766
In the 1880s, San Francisco attracted some of the most prominent regional and nationally recognized artists of the day, including William Keith, Thomas Hill, Albert Bierstadt, and George Inness, among many others. The city's California School of Design similarly attracted some of the most notable artists and instructors who would have a profound impact on Guy Rose's early career, including most significantly Soren Emil Carlsen, who became director of the school in 1887. "[Carlsen] brought a distinct sense of modernity to his still-life painting, the genre for which he remains best known...[he] applied pigment broadly and in dramatic, baroque contrasts of light and dark. Carlsen was sensitive to lost edges in pictorial space, where, for example, the silvery scales of fish give way to an enveloping dark, empty background...In certain discreet passages, the sense of illusion in a painting by Carlsen is balanced with the concrete, sensual presence of pigment. His confident application of thick, dark paint and brilliant highlights betrays a debt to Velázquez and certain of the Dutch masters. His fluid articulation of paint not only impressed Guy Rose but served as a precedent for the considerably looser surfaces Guy would later come to understand and master." (W. South, Guy Rose: American Impressionist, San Francisco, California, 1995, p. 14)
Rose's Still Life with Onions, Peppers and Basket is deeply indebted to the teachings of Emil Carlsen and the broader influence of one of the most influential instructors of the day, William Merritt Chase. Rose's exquisite mastery of detail and texture is achieved through a bold composition dramatically juxtaposing stark light against a cavernous space of unknown shadow and depth. The use of a subtle diagonal line in the foreground to indicate the edge of a table serves to enhance a more dynamic picture plane and invite the viewer into what had traditionally been a more static genre. Having remained in the artist's family until the present time, Still Life with Onions, Peppers and Basket is a rare and exceptional early example from the most renowned California Impressionist.
This painting will be included in the catalogue raisonné on the artist being compiled by Roy Rose and the Irvine Museum.
By descent in the family of the artist to the present owner.
Christie's Spring 2018 auction of more than 2,000 items will continue the long legacy of Rockefeller philanthropy
An expert introduction to the movement, from its origins in France to its flowering on a private island off the coast of New En..
From Yves Saint Laurent in Paris to David Hockney in New York, the must-see shows for the second half of 2017
Exceptional pieces offered in the second half of 2017 in Paris, New York and Geneva

subset: commoncrawl


text:
hey guys! This is iProm Part 5
Warning: This is the last part so no more rushing me.
Freddie: YOUR my blind date?
Sam: Unfortunately.. yes.
Freddie: Why would Carly think we should get back together?
Sam: I dont know maybe because.. well what do you think?
Freddie: Well.. I will admit, I was a little more happy when I was with you.
Sam: Aww (hug)
DJ: Ok.. and now were going to bring it DOWNNNN.......
(Slow Music starts playing)
Seddie: (awkward silence)
Sam: This is the song that played when we first kissed...
Freddie: Yeah....
Carly: You're such a good dancer.
Devon: Thanks, your not so bad yourself. Hey, your really hot tonight.
Carly: Well so are you.
T-Bo: MISS BENSON!
Mrs. Benson: WHAT DO YOU WANT YOU FREAK?
T-Bo: Do you wanna dance?
Mrs. Benson: Your kidding right.
T-Bo: Imma good dancer. WATCH. (twirls mrs. benson)
Mrs. Benson: You are pretty good.
T-Bo: Thanks.
Freddie: S.. Sam?
Sam: F..Freddie..
Freddie: D.. do you want to dance with me?
Sam: Wouldn't you much rather dance with Carly... (in a depressed voice)
Freddie: No. I'm asking to dance with you.
Sam: Ok.
{Seddie Slow Dance}
Freddie: Listen Sam..
Sam: Yeah?
Freddie: Breaking up with you was the stupidest thing I've ever done.
Sam: (shocked face)
Freddie: Do you want to get back together?
Sam: Well... I dont want it to go to fast. But yes, I do want to get back together though.
Freddie: Maybe after a few days we will talk about it and see.
Sam: Ok
{Slow Kiss}
Did you guys like it? I will be making a sequel to this called iDon't Know. Will you read iDont Know??? :)


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Cat Catches GIANT Fish - PetTube
PetTubePlanetsFunniestAnimalsPublished: June 24, 2016362 views
Published: June 24, 2016
Check out more funny pet videos at http://pettube.com
This feisty feline celebrates the catch of the day!
From the producers of television's "Planet's Funniest Animals" and "Funniest Pets and People" comes PetTube, a channel and website dedicated to pets and the people who love them. PetTube gi


text:
WATCH: Did He Really Just Hit Those High Notes?
Photo Credit: Youtube
Caleb Johnson is a finalist on this seasons American Idol. He nailed it with his rendition of the classic "Dream On' by Aerosmith. It is very difficult to belt out those last notes that Steven Tyler does so well. But Caleb sang them so well that everyone's jaws were dropped! I think he may win, we will find out tonight!


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In case you did not get a chance to catch Apple's live feed of the event today, luckily, I did, as did many actual tech journalists. All the details and specs about the two new iPhones, the iPhone 7 and 7 Plus, were revealed in typical Apple fashion. Also, a new product, Apple AirPods, was released in addition to quite a few product upgrades. Several new games were showcased that the new, more powerful, iPhone can handle. One game is from the Super Mario Brothers line.
Continue reading "Apple Unveils the iPhone 7 and iPhone 7 Plus and More! " »


text:
The eurosceptics have the best lines again
There are eerie parallels between the euro debates in the UK in the late 1990s and in the northern eurozone today. Back then, the anti-euro campaigners in the UK highlighted the shortcomings in the construction of the eurozone – an analysis that turned out to be correct – and also correctly predicted the euro would require a political union to succeed in the long run. There was also emotional stuff about the Queen on banknotes. But it was an internally consistent position. I did not share that view but I recall admitting back then that if you do not accept political union, logically you should not accept the euro either. There was never a purely economic case for the currency.
The pro-campaign in the UK,
by contrast, was pathetic. It was based on the notion that the anti-campaign exaggerated their case, and that a single currency was not such a big deal. Anyway, supporters argued, it was in Britain's interest to remain in the heart of Europe, whatever that meant. I challenged a young pro-euro MP at the time. His answer was that in Britain advocating a political union was impossible; hence, a campaign to play down the implications would be a good second best.
Fast forward 13 years. Last week, more than 200 economists signed a petition organised by Hans-Werner Sinn, head of Germany's Ifo economics institute and the most eurosceptic voice among the country's economists. He has not yet advocated a withdrawal from the euro, but his recommendations are not consistent with the eurozone continuing in its present form. The petition focused on the banking union, and why this would open the door to a fiscal union and large-scale burden fiscal sharing.
Other economists then launched a counter-petition arguing that a banking union is a logical extension of a monetary union. That in itself is correct. But, like the pro-euro campaigners in the UK in the 1990s, they played down the implications. They said a banking union is nothing to be afraid of; there would be no transfers; there is no question of Germany taking on anybody else's banking debt.
I disagree with the views of Professor Sinn and his group. But I have to admit their position is internally coherent. It opposes transfers and ultimately accepts the break-up of the eurozone.
The position of the so-called pro-euro movement, however, is a lot less coherent. A properly constructed banking union will, of course, imply permanent transfers, as would a properly defined fiscal union. The idea that you can resolve the eurozone crisis without transfers of any sort is delusional. Since the German banking system is relatively stronger than Spain's, a banking union would, of course, imply a loss for Germany. If you have joint deposit insurance and joint banking recapitalisation, surely transfers will take place – just as they now take place inside countries themselves.
The pro-campaigners in Germany, as in Britain 13 years ago, pretend to be pragmatic. This is why they agree with Chancellor Angela Merkel's rejection of eurozone bonds. They are not a sellable proposition in Germany, they say. Likewise, they agree that economic adjustment in the eurozone cannot be symmetrical, again on the grounds that it is not a sellable proposition. For the same reason, they also support all the deficit rules, despite the fact that they have never worked. They also reject changes to the European Central Bank's mandate. With pro-Europeans like that, who needs eurosceptics? Hilariously, several professors signed both documents.
The proposal by the German council of economic advisers for a so-called debt redemption fund falls into the same category. It is another tribute to the shrine of pragmatism. It appears pro-European but, on closer inspection, it is not going to resolve the debt crisis. A debt redemption fund is an instrument to pool a portion of each country's debt in exchange for the issuance of a eurozone bond. But the idea is for the debt to be repaid, so the bond issue will ultimately expire. That may be fine for relatively small amounts, but Italy's "excess" debt is about €1tn. The idea that Rome can repay its excess debt in 25 years is insane, unless you assume a miraculous and sustained pickup in economic growth.
As in Britain in the 1990s, today's pro-Europeans are incapable of defending the idea that a monetary union requires a banking union, a minimally sufficient fiscal union with tax-raising powers, and a political union with a legislative mandate independent of member states. The eurosceptics are saying they do not want any of this. The pro-Europeans want to obfuscate.
Much as I hate to admit this, Prof Sinn's group is intellectually more consistent than the group whose ultimate goals I share.
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September 13 - 16, 2017
Sands Expo | Las Vegas, NV
Las Vegas, the Entertainment Capital of the World. There is something for everyone here— all you have to do is find it. And we're here to ease that search so you can choose the Vegas experience you want to have. This visitor guide highlights the best of Las Vegas with suggested things to see and do while you're in town for International Vision Expo West. Indulge in music, theater, food, shopping, spas, nightlife or whatever catches your eye. We hope you enjoy your trip.
The Following document pinpoints specific areas in Las Vegas with suggestions on what to do and see while in town for International Vision Expo West. View this document in:
When you arrive in Las Vegas, you immediately feel the energy that makes it so iconic. There is something for everyone, which makes Las Vegas a grand stage for Vision Expo West.
Entertainment runs the full spectrum—from acrobatic masterpieces, to live concerts, to breathtaking views and rides. You can spend your days shopping international brands, lounging poolside and indulging in well-deserved relaxation at the spa. Evenings may find you sampling 5-star cuisine from the hottest chefs, dancing the night away and, of course, trying your luck in the casinos. The world's most dazzling destination is a perfect backdrop for attendees at Vision Expo West.
The Las Vegas restaurant landscape is one of the most exciting in the world. With so many extravagant, splurge-worthy dining experiences available in Las Vegas, it can be difficult to choose. Here are a few tried and true options for every adventurous foodie.
Check out LasVegas.com's dining section for more information on where to eat.
While the Strip takes center stage, there's a whole world of activities for those eager to venture beyond the glittering lights. Twenty-four hours in a day hardly seems like enough. Wall-to-wall Las Vegas activities mean you get more in your getaway. Check out these Things To Do in Las Vegas where you'll be guided down the path of exciting things to do.
With nearly 40 million people visiting Las Vegas yearly, it has become a hot spot for international retailers. Travel the world without ever leaving the Strip. Crystals at CityCenter. The Forum Shops at Caesars. The Grand Canal Shoppes at The Venetian/The Palazzo. The Miracle Mile Shops at Planet Hollywood. The list could go on forever, and trust us, it does. In fact, it's fair to say that shopping in Las Vegas beats shopping anywhere else in the world. Learn more about Las Vegas Shopping here.
After a long day on the show floor, you deserve an evening off. Kick off your shoes and enjoy a blissed-out day at one of Las Vegas' spas. And whatever atmosphere helps you relax, Las Vegas spas surely offer it, take a look at what the spas have to offer here.
Vegas is the perfect place to tee up on your perfect course. Follow our guide to match your golf preferences to the perfect course. You'll find some of the finest championship courses in the world right in Vegas.
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The coastal and sea areas of Hawaii and Southern California are important for recreation and commercial activities, and are home to a variety of marine plants and animals, including whales, dolphins, seals, sea lions, sea turtles, corals, invertebrates, sea birds, and multiple fish species.
Minimizing impacts from training and testing on the marine environment is an important goal for the Navy. In its commitment to environmental protection, and in compliance with existing laws, permits, and authorizations, the Navy follows strict guidelines and employs measures to reduce potential effects on marine species while training and testing.
Funding Independent Research
The Navy is a world leader in marine species research. For more than a decade, the Navy has funded research and partnered with universities, research institutions, federal laboratories, and private researchers around the world to increase the understanding of marine species distribution, physiology, and behavior. This scientific research helps environmental regulators, scientists, and the Navy to:
Observing the Area Prior to Activities
Posting Qualified Lookouts
Establishing Mitigation Zones for Seafloor Resources
Navigating Safely
Reporting Monitoring Results
Protective Measures Assessment Protocol
Quantifying Acoustic Impacts
Ensuring Access and Public Safety
Public Safety Measures
The safety of military personnel and the public is of utmost importance. The Navy observes every precaution when planning and conducting training and testing activities. Some precautionary measures in place include:
These measures are intended to ensure public safety during scheduled Navy training and testing activities. These measures, along with the cooperation of the public and commercial and recreational users of the air and sea spaces, enable safe training and testing. The Navy publishes notices to mariners with location, activity, and duration information. Mariners are requested to read and adhere to the published notices.
Thorough environmental and safety reviews are conducted for all test systems. Prior to going into the water, most systems go through land-based testing, and many have been tested in smaller fresh water areas or tanks. After an initial review, modifications are made, as needed, to minimize the potential impacts on public safety and the natural environment.
U.S. Navy | Navy Recruiting | FOIA | No Fear Act
Veterans Crisis Line | Accessibility/Section 508


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Doctor insights on:
Cauterization Risks
What is the definition or description of: cauterization?
Heat treatment: Cauterization is the term for the use of a device which uses heat to treat tissues, most commonly at surgery to diminish bleeding but also externally for bleeding such as nasal bleeding. It can also be used to remove lesions on the skin like warts and moles. ...Read more
Cauterization (Definition)
Cauterization is the term for the use of a device which uses heat to treat tissues, most commonly at surgery to diminish bleeding but also externally for bleeding such as nasal bleeding. It can also be used to remove lesions on the skin ...Read more
What is a cervical cauterization?
CAUTERIZATION: A procedure to eliminate chronic cervicitis and possibly ca in situ done by gyne md. ...Read more
Should i go to school tommorow after Cauterization today?
Clarify please: Please advise what part of your body was cauterized. Can re-ask with clarification. ...Read moreSee 1 more doctor answer
How can you manage pain after a hysterectomy and cauterization procedure?
Combination: Usually narcotics are given (tylenol (acetaminophen) with codiene, vicodin, percocets, etc). Take these as recommended but remember you can also take Ibuprofen or naprosyn (advil or aleve) and the combination work better together. Try to move around, avoid over-exerting yourself, & call your doc if your pain is worsening. Sometimes a change in meds helps also. Take good care of yourself. ...Read more
How painful is nose cauterization?
Depends: Nose cauterisation is not pleasant but most doctors spray local anaesthetic before cauterisation and that helps to decrease the pain. ...Read moreSee 1 more doctor answer
How much does nose cauterization hurt?
Variable: Pain is relative and what one person says is a slight twinge may be quite painful for another. That said, i usually apply a topical anesthetic first. This tends to make the procedure more comfortable. ...Read more
How long does the test good for? How many years? I had a test done in october . Cardiac cauterization how long is this test good for. Is it done every year
Only when needed: A cath should only be performed if there is a good chance of finding something worth "fixing". It should be guided on symptoms and the results of less invasive tests, particularly stress tests. ...Read moreSee 1 more doctor answer
Is it effective to remove hemorrhoids by cauterization?
Depends: Small internal hemorrhoids especially if the major symptom is bleeding can be treated with irc (infrared coagulation) which is a form of cauterization. ...Read more
When after nasal cauterization do I have to wait to blow my nose?
Nose cautery: After nasal cautery, you really want to let the nose heal. Try to avoid blowing your nose for 2-3 days, longer if more bleeding stirs up after cautery. Keep the nose moist with saline, too. This facilitates the healing if the lining of the nose after treatment. You can also prevent drying of the nose by putting a little vaseline just inside the openings if the nose. ...Read more
Any connection between a nasal cauterization and then face pain following that?
Very common: After nasal cauterization, you may feel itching and pain in your nose and face for for 5 to 10 days. If concerned see your doctor for post-op appointment. ...Read moreSee 2 more doctor answers
- Talk to a doctor live online for free
- Wart cauterization
- Uterine cauterization
- Cauterization of the heart
- Ask a doctor a question free online
- Cauterization complications
- Cauterization and warts
- Nasal congestion after cauterization
- Pregnancy after cervical cauterization
- Cauterization


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Hi All. I was just curious if anyone knew the answer to the following:
I picked up a used WRT610n V1 from a friend of mine to replace an aging WRT54g. For whatever reason the the router's web interface is accessible from the WAN. For now I forwarded port 80 to a non-existent LAN IP and it is now not accessible from the WAN. I was just wondering if I'm missing something really stupid. Its internal firewall is turned on and the only forwarding rule that I currently have on there is to that one non-existent IP. Is there something I'm missing or is this a known issue with the stock firmware? I would rather not have it show up on a port scan from the WAN IP if possible (this was tested from a device not connected to my LAN btw). I can't seem to find any info on google.
11 Replies
Feb 18, 2012 at 1:40 UTC
Page 30
Remote management access
Disable it!
Feb 18, 2012 at 1:47 UTC
It was disabled by default. Just wanted to mention that I am on the latest firmware too.
Feb 18, 2012 at 1:55 UTC
Have you tried to reset it to factory defaults after FW upgrade?
Feb 18, 2012 at 2:45 UTC
Yes I have. It's been reset a couple different times in fact.
Feb 18, 2012 at 2:50 UTC
Allowed Remote IP Address If you want to be able to
access the Router from any external IP address, select Any
IP Address. If you want to specify an external IP address or
range of IP addresses, then select the second option and
complete the fields provided.
If you set remote address to something like 18.104.22.168, is still avaialble form the internet?
Feb 19, 2012 at 6:02 UTC
Interesting, I enabled remote management (even though I dont use it), changed it to use 127.0.0.1 on an alternate port, then it didn't show up, then it did after I turned it off, then I turned it back on and it still shows port 80 being open (which I had to forward to my brother in law's PS3 yesterday for a game.). Port 80 still shows on the port scan though obviously I cant connect to the router via the Internet as it's being forwarded to the PS3. The PS3 was not on during that time.
Feb 19, 2012 at 6:30 UTC
Obviously this router has a lot of bugs inside :-)
Feb 19, 2012 at 7:19 UTC
I think I agree with that lol
Feb 20, 2012 at 7:10 UTC
Obviously this router has a lot of bugs inside :-)
Ripoff clone router that is not running a real Cisco OS? I seem to remember a story about fake Cisco equipment awhile back.
Feb 20, 2012 at 7:25 UTC
Unfortunatly, this is old bed linksys, before Cisco got them under control.
V1 of device
Feb 27, 2012 at 12:55 UTC
A ripoff clone router ?Lol. I was able to load the latest stock firmware on it without a hitch. Could that even still work if its a knock off? I suppose it could but still...
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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Difference between the two Black Codes we put into place to regulate what slaves could and could not do, they were another way to reinstate slavery following the Civil War, dealing with social controls. While Jim Crow Laws deprived blacks of civic and political power, such as segregating schools and forbidding intermarriage between blacks and whites. Jim Crow laws were laws that were established to separate the whites and the black races in the South of America.
Jim Crow Laws were to create "separate but equal" treatment.
They condemned black citizens to inferior treatment and facilities. What are the Jim Crow Laws? Segregation Education was segregated
Drinking fountains
Etc. Timeline 1875-1975
1875 March 1 - Congress passes Civil Rights Act of 1875, prohibiting discrimination in public places.
1913-1921 - Woodrow Wilson institutionalized segregation in the federal civil service. By the end of World War I, the District of Columbia was thoroughly segregated as well.
1914 - Every southern state and many northern cities had Jim Crow laws that discriminated against black Americans. Black Codes Vs. Jim Crow Laws Jim Crow Laws Black Codes What are the Black Codes? Black Codes were a series of restrictive laws
They were designed to restrict freed black s' activity and ensure their availability as a labor force since slavery had been abolished.
Many states required blacks to sign yearly labor contracts, if they refused, they risked being arrested as vagrants and fined or forced into unpaid labor. Black Codes Timeline 1863 Ten percent plan
1865 Reconstruction
14-4-1865 Lincoln's Assassination
Late 1865, early 1866, Southern States passes black code
1868 the 14th Amendment was passed
1869 the 15th Amendment was passed Sources http://www.sitinmovement.org/history/america-civil-rights-timeline.asp
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Black Codes vs. Jim Crow Laws
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Movies: Too many to name. But I'm a huge Liam Neeson fan.
Shows: Arrow, The Flash, Gotham, Big Bang Theory, That 70's Show, Smallville.
Food: Italian, Mexican, Chinese.
The past, the present, the future. What I'm doing with my life, what has happened to my life. How I'm going to die alone.


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GraphicRiver Creative Nature Design Elements 2533387
Stock Vector \ Decorative \ Decorative Symbols
Design \ Elements \ Orange \ Editable \ Formats \ Textured \ Shapes \ Vector
Stock Vector - Creative Nature Design Elements 2533387 by antishock @ GraphicRiver
Stock Vector \ Decorative \ Decorative Symbols
Nature design elements: glossy metal circles framed with green and orange leaves, arrows and random textured shapes.
EPS10 Fully editable vector. EPS file provided only. All design elements included in EPS file, no files of other formats.
Layered: No; Graphics Files Included: Vector EPS; Minimum Adobe CS Version: CS.
Abstract Background; Triangle Geometric Concept; Triangle Geometric Concept; Merry Christmas modern cards - abstract baubles; Christmas Background; Christmas Background; Happy New Year Concept.
Keywords: arrow, autumn, background, blue, circle, design, element, gray, green, leaf, maple, metal, nature, orange, red, round, spring, summer, yellow.


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- Home
- Votive, White
White Votive
Handmade White, Unscented Votive. Approximate burn time 15 hours.
Each batch is hand made; Color pigments may vary with each batch and from the photo.
Unscented votive which allows you to empower by anointing with your oils, herbs and energy.
Votives either slightly tapered or straight cylinder.
White is known for: Element - Spirit; Moon/Lunar; Goddess; Truth, Sincerity, Spirituality, Psychic Development and Abilities, Sincerity, Divination, Channeling, Meditation, Inspiration, Protection, Peace, Purification, Cleansing, Calling on Spirit Guides, Higher Self, Divination, Wisdom, Prophecy, Consecration, Healing, Angels, Vision, New Beginnings, Birth, Marriage. White is a neutral color, can be used to replace or in substitute of another color.
White is used in Energy/Chakra Work on the 7th Chakra - Crown (Purple is also used on the 7th Chakra).
Place in flameproof holder and trim wick to 1/4 inch to enable better burning quality and time.


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3 Min Read
* TSX ends down 144.95 points, or 1.2 pct, to 12,178.66 * Index hits lowest level since January * Suncor, golds lead names on downside By Claire Sibonney TORONTO, April 4 (Reuters) - Sinking commodity stocks led Canadian equities to a near 12-week low on Wednesday, a day after the U.S. central bank crushed hopes for more monetary stimulus and as a weak Spanish bond auction signaled the effects of earlier European funding operations may be waning. Big names on the downside included Suncor Energy, which plunged 4.5 percent to C$31.26, Goldcorp, which lost 4.9 percent to C$40.98 and Barrick Gold, which slid 3.2 percent to C$41.16 as the price of gold fell to its lowest levels in nearly three months. "We've seen a steady decrease in optimism that the Fed would embark on easier monetary policy in recent weeks and the gold group has been under pressure ever since, so has gold for that matter," said Elvis Picardo, strategist and vice-president of research at Global Securities in Vancouver. Energy companies were also hit by retreating oil prices, which ended about 2 percent lower, after testing key technical support levels. U.S. government data showed crude stockpiles in the world's top consumer jumped last week to a nine-month high. Suncor in particular however was also hurt by news that its output was down more than 40 percent in March from February. The Toronto Stock Exchange's S&P/TSX composite index ended down 145.02 points, or 1.18 percent, to 12,178.59. Earlier in the session, the index hit its weakest level since Jan. 13. Comments from European Central Bank President Mario Draghi that the euro zone's economic outlook is subject to downside risks related to the debt crisis also dented risk sentiment. The flight from stocks and commodities followed through on Tuesday's selloff, after the Federal Reserve's minutes from its March meeting suggested the appetite for a third dose of quantitative easing, so-called QE3, has decreased. Meanwhile on Wednesday, Spanish borrowing costs jumped at bond auctions, raising concerns that the rally in troubled peripheral sovereign debt sparked by the European Central Bank's two Long-Term Refinancing Operations may be coming to an end. "We've seen headline risk out of Europe recede very sharply in recent months so anything that comes out that again refocuses attention on the issue tends to have a negative impact in markets," added Picardo. In other individual company news, shares of Canadian home-improvement retailer Rona Inc slipped 1.2 percent to C$10.35 after the company said it was not for sale. WestJet Airlines bucked the broader market trend, climbing 2 percent to C$13.62 after reporting it flew fuller planes in March despite higher fares.


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|Fragment of painting
for watercolor class "Fish. Bubbles. Easy watercolour paintings"
by Olga Peregood
- we made an exiting 1 wash and turn it into water fish can't live without
- learn colors
- mixed them together
- made beautiful watermarks
- apply salt effect and turn it into bubbles
- and tissue for corrections
- composition
- and harmony by direction and color
- how to be loosed and confident while painting
|Original watercolor painting "Fish. Bubbles" by Olga Peregood
Come and watch Trailer and Join Class via this link - https://youtu.be/Emk_hb4XEeE


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Goodyear Eagle F1 Asymmetric 2: Concentrated technology
To take the lead in a highly disputed category, the Goodyear Eagle F1 Asymmetric 2 inaugurates an impressive set of technologies.
On the same topic
> Improved braking
The Eagle F1 Asymmetric uses ActiveBraking which is characterised by a crossed plies carcass structure and a high-surface silica rubber compound.
This technology considerably increases the ground surface under braking pressure. This enables stopping distances to be reduced. A traditional tyre however does not change much during these phases.
In addition, a new rubber compound comprised of high molecular weight polymers, very high silica content and a traction resin derived from motor sports. All this improves braking performances on wet roads.
> Better driving precision
The Eagle F1 Asymmetric has a crossed plies carcass structure which stiffens the profile. The noted advantages are greater driving precision and better road holding and braking on dry surfaces.
> Optimised energy efficiency (FuelSaving)
To reduce consumption (and hence CO2 emissions), the Eagle F1 Asymmetric 2 offers 3 solutions:
Ø Reduced weight with less material and optimised structure to limit heating and rolling resistance.
Ø A new type of high-performance silica that reduces heating.
Ø Aerodynamic sidewalls.
The stiffness of the tread provides even pressure and distribution of stiffness over the whole contact patch, allowing for potentially greater mileage.
In view of future tyre labelling, the Eagle F1 Asymmetric 2 has been optimised to emit less noise.
Goodyear Eagle F1 Asymmetric 2
o Performances
o Dimensions
Articles in the same header
- Discoverer Winter: the new winter tyre for SUVs from Cooper Tire
- Kristall Control SUV: a new winter tyre by Fulda
- Eskimo SUV 2: Sava launches a new winter tyre for SUVs
- AllSeasonContact: The new all season tyre from Continental
- Yokohama presents two new winter tyres for its BluEarth and IceGuard ranges
- Apollo launches two new tyres for the European market at Geneva
- Yokohama announces the return of the A008P
- Ecorun A-A: Falken unveils its tyre for Toyota hybrid vehicles
- Yokohama extends its BluEarth range with two new summer tyres
- Michelin Pilot Sport 4 S: a new benchmark sports tyre?


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Tired, annoyed, frustrated? These would be some of the basic symptoms of Shade Syndrome. Trying to find that perfect pair of sunglasses is a delicate dance, that can sometimes leave you annoyed and frustrated. Here's a quick guide for some handy tips to finding that diamond in the rough / which in this case is your perfect pair of sunglasses that best compliment your shape.
Heart-Shaped: Wearing your heart on your forehead, opposed to your sleeve? With having a wide forehead and cheekbones in conjunction to a narrow chin, cat-eye sunglasses are your go-to frame style. The best attribute of cat-eye sunglasses is the illusion of lift. I refer to it as a face-lift in a frame. For those of us who don't want to go under the knife, cat-eye sunglasses are the purrrfect fit for you. Meow!
Sunglasses Brand: Cheap Monday.
Oblong-Shaped: Why the long face? Can't find that perfect pair of shades? With having the facial features of a long face, narrow chin, and cheeks, big over-sized sunglasses are your true match. The bigger the better! One great feature for over-sized wrapped sunglasses is the fact that when worn, you will give off a cool, chic, mysterious vixen look, and who doesn't like a good Nancy Drew novel?
Sunglasses Brand: Marc by Marc Jacobs.
Square-Shaped: Having problems thinking outside the box? With broad being a key word, from having a broad forehead to a square jawline, you have your pick at a broad range of differently styled sunglasses. From round and oval to cat-eye frames, these will all compliment your facial features best. Sunglasses to stay away from would be any sharp geometric shaped frames.
Sunglasses Brand: Left to Right – Victoria Beckham, Marc by Marc Jacobs, Karen Walker
Oval –Shaped: Just like a supermodel in a Victoria Secret ad, you can pretty much pull off any style. With that being said, go out and invest in some great sunglasses that come in all sorts of different designs. Dare to be daring! Like in these Alexander McQueen two tone metallic sunglasses.
Sunglasses Brand: Alexander McQueen
Round-Shaped: Around and round we go… The sunglasses that will best frame your round face would be any style of frames that are wide and rectangular. Opposites attract! And in this case it is your round face, with a perfect pair of wide, geometric styled sunglasses. No curves here!
Sunglasses Brand: Stella McCartney
We all come in different shapes and sizes. It's what makes you, Y-O-U! With all of us given an uniqueness about ourselves, it can sometimes be frustrating when trying to find our true fit. I hope this guide can be a quick fix to your dilemma. Opposed to throwing shade, we need to start wearing shade. Great, amazing, fabulous, look at me shade!
All sunglasses are purchasable at shopbop.com. Be sure to check out their website for great, on trend, styles of fabulous looking sunglasses from a variety of different designers.


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Arlington, VAUSA
Hometown: Bangor, ME
July 12, 2012
Currently, our organization uses a custom in-house spun oo-mod/perl content management system to hook up our million users with the tools they need to give congress a hard time when it does something we, both as an organization and as a broader movement, think harms or otherwise loosens the footing of the hard working middle and working classes in the country, with a focus on immigration policies. On a more personal note, I've used perl to pick apart maps and draw new ones, interpret pixels as instructions for building ray-tracing scripts, serve as a tentative back end for app services in regards to game ideas that I never find the time or energy to complete, et cetera.
Hallo; long time perl user (12-14 years or so...) for a political advocacy group, looking to get out of the gorram house once in a while and serve other nefarious purposes.
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Pet Sitter in Orillia
Hello, my name is Amanda. I am 18 years old and am currently looking for an animal job. I have had quite a bit of experience with animals, personal and job-wise. I have animals of my own (2 dogs, 4 cats, 1 bird) and I have also worked in a pet store (5 months experience). I really enjoy being with animals too!
Pet Sitter Details
|Email Address
|Phone Number
|Provider Type
Dog Walking
Pet Feeding
Pet Sitting
Notes made here will only be seen by you. If you want to message the owner of this listing
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text:
|July 03, 2017, 16:54
Bronze Contributor
FALaholic #: 74557
Join Date: Aug 2014
Location: Foothills of the Blueridge Mountains
Posts: 3,812
Finally got all my helmets upgraded and have more than enough till next generation obsolete what have now. But wife and I each have a pair of ACH MICH high ear with N Rails, have standard ACH MICH in truck emergency kits and lots of spares. All have night vision mounts and have six complete night vision arms and adapters. My primary has an early Gen 2 PVS-14 monocular and on five others have a selection of I.R. and Thermal devices attached from elegantly as the PVS-14 attaches to J Arm to one with short section of pic rail attached to J Arm and device attached to pic rail and down to a couple that required use of duct tape. Funny thing is have a Bushnell I.R. monocular paid $50 duct taped to J Arm, is rated for 10 yards and in the house works stunning with the ambient light after we shut down for evening or if pull plug on all lights the illuminator does great room to room. Step outside in dark and 10 yards is about its functional range.
Been playing a lot with the new primary as added a Peltor noise cancelling hear through headset and dual radio capability. Am not really satisfied with my monocular anymore. Compared to my ATN Gen 2+ CGT night vision scopes it lags well behind them in performance. Have no desire to spend Gen 3 money but beginning to see a lot of Gen 2+ PVS-14's in the $1,199 to $1,799 range. Especially if don't need the mounting brackets. While don't need to spend any extra money right now a guy came into LGS with a Armasight NYX-14 HD MG Gen 2+ had purchased then when his wife saw the credit card bill flipped out. Claims has had out of box once and sales receipt, shipping documents show he has had in his possession for two weeks. Also know he has been to every LGS in town, pawn shops, has listed on Craigslist and fleabay. Got his number and think $900 would part him with it. He dropped price to $1,000 in LGS and his fleabay auction has opening bid of $999 running with over seven days left and no bids. Awfully tempting to call and make a cash offer and break into some locked up funds. Know there are lots of guys here that refurbish and resell night vision. Looking to upgrade by winter when time changes so what should I expect to pay for a Gen 2+ monocular compatible with military mounting system.
A time will come when people will not listen to accurate teachings. Instead, they will follow their own desires and surround themselves with teachers who tell them what they want to hear. 2 Timothy 4:3 G.W.T.
"If it is worth doing once...it's worth doing it a few more times to get it right." Stimpsonjcat
|July 03, 2017, 22:58
Gold Contributor
FALaholic #: 129
Join Date: Jul 2000
Location: phx az
Posts: 14,599
DrTrumpet was selling refurb PVS14's in the $1500 range - I got a backup for my OMNI unit, I think he put a GenIII ANVIS tube in a new 14 housing - only real difference I can tell between his $1500 unit and the $3500 OMNI tube is manual gain on the latter and auto gain on the former - unit works GREAT - ought to ping him and see if he's still doing them, great value IMO.
|July 04, 2017, 08:47
FALaholic #: 74891
Join Date: Oct 2014
Location: Tioga County, PA
Posts: 980
Huey you seem to have tons of money so stop farting around and get some PVS-21s. I use them several times a week fitted to a Team Wendy helmet and they are worth it. Light, compact, great vision, plenty of adjustments to make for an easy fit. $20K on sale.
|July 04, 2017, 11:45
FALaholic #: 31665
Join Date: Sep 2007
Location: on top of a wind swept ridge
Posts: 3,828
Jim Rawles of Survivalblog https://survivalblog.com has a ad on the right side bar for PVS14 (I think it is his side company) usually has good price on the equipment (although I never really shopped for night vision gear w/ my meager wallet)
Far better it is to dare mighty things,
than to take refuge with those timid spirits
that know neither victory, nor defeat.
Teddy Roosvelt
Pray for peace, but prepare for war.
Winston Churchill
|July 08, 2017, 17:28
Bronze Contributor
FALaholic #: 74557
Join Date: Aug 2014
Location: Foothills of the Blueridge Mountains
Posts: 3,812
I don't have plenty of money, just a modest but steady toy budget that if thrifty with seems to keep adding toys. Yes, could order a $18,000 set of panoramic Gen 3 goggles but it would kill a years budget of hunting bargains and would only have one very expensive night vision kit that would feel like needed to be locked in vault or Faraday box. Am the type that assumes SHTF at very unexpected times. Why have a bug-home kits in all vehicles.
Have discovered Gen 2+ night vision does pretty much what I need. Often find someone who bought an item and used once then unexpected bill or angry wife put it in my hands at half street price with less than 10 hours use. Right now am buying lots of high quality 6.8 barrels from people switching back to 5.56 due to availability of ammunition. Found deal on new primed brass end of 2015 and ended up with 15,000 cases from one company dirt cheap. Found 5,000 loaded milspec 90 grain Gold Dots on clearance then 6.8 costs shot to the moon. It's a constant search for deals, willing to be one generation off latest technology that allows me to have a helmet with hear through ear muffs, dual radio capability and night vision. If ordered a new ACH MICH high ear with N Rails, MSA/Sordin dual radio headsets, Team Wendy suspension/padding and Gen 3 night vision it's a $25,000 to $30,000 helmet and would only own one and have to give to wife as won't let her take my seconds. Can be frugal with lightly used, 1.0 to 1.5 generations off latest tech and have $2,500 in a fully equipped quality helmet. That means wife and I both have one almost current, like new primary kit. Then we have a a little older (say Gen 2 optics, single channel headset) kit for trucks and backup. The workable kit that's close at hand when need beats the top of line kit locked up in safe or at home when on vacation. Why always lurking and looking for deals on lightly used and refurbished equipment. Have to add a radio or two to each vest, can buy three good used Icom handhelds for less than one new. Can buy five good public service Motorola's. Rather have three of last year's model or five police duty radios than one of current model plus accessories are cheaper for the older. Especially when going to open up to do a MARS/CAP free band mod that voids warranty first day to unlock transmit abilify across entire operating range.
Caught a one day sale with Leupold Tracker thermal monoculars for $399 and free shipping. Know for a fact, most refurbs are returns from remorseful shoppers. Factory goes through, does more testing than new, repackages with warranty and have two Loopy thermals for price of one. It's how am wired and will always be even if had a seven figure toy budget. Have a lot of hobbies, just added an EKG and defibrillator to home med kit from Iraq MASH unit surplus. Also scored a good used weather radar that doesn't need repaired. It will get packed in a Faraday box with my spare weather fax and other forecasting equipment. If buy new at retail would have one super high speed, low drag operators kit for an old man to guard and care for. Rather have a pile of beaters I can field without remorse. Used my helmet with duct taped Leupold Tracker last night. The Norotoros mount has enough adjustment it flips down in perfect eye position then up out of the way, $70 helmet, $30 N Rail kit, $35 Team Wendy pads, $40 Norotoros mount, $399 Loopy thermal and $175 single channel hear through headset. Used a PTT kit with splitter for second radio with Safariland controls and helmet is better than most solders had in Iraq for under $1,000 and 80% functionality of a $25,000 Uncle Sugar kit that a real operator uses.
A time will come when people will not listen to accurate teachings. Instead, they will follow their own desires and surround themselves with teachers who tell them what they want to hear. 2 Timothy 4:3 G.W.T.
"If it is worth doing once...it's worth doing it a few more times to get it right." Stimpsonjcat
|July 13, 2017, 06:39
FALaholic #: 66611
Join Date: Jun 2012
Location: Wells, Vermont, USA
Posts: 720
|Today, 21:40
FALaholic #: 20538
Join Date: Jul 2006
Location: Dallas-Fort Worth
Posts: 4,434
After a while they shut down and moved the Gen 3 line to Arizona but kept making Gen 2 stuff there for quite awhile longer. Has to be good to be kept in production that long.
Me? I'm kinda a PVS-17 fan.
There's only two reasons we showed up here, to party and kick some ass, and we're almost out of beer!
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Wrestle And Fuck teamed up Champion wrestler Rick Bauer and Muscle God newcomer Max Summers. Max is the larger man - not that Rick is small by any means - and although more muscular in stature, his grappling skills were no match at all for what Rick brought to the fight. No matter how hard he tried, Max just couldn't submit the smaller, and yet more agile stud. One gets the impression that Rick was actually playing with his more ample opponent. Especially when he finally tired him out of wrestling and had him laying on his back, legs spread wide open, waiting for Rick's main muscle to enter his hugely muscled butt.
Tags: fighting (14), wrestling (76),


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As Savage walked through the press box area, the media had gathered, but he didn't have much to say.
"I want to wish you all a Happy New Year", Savage said, "I'm glad we were able to sign Leigh Bodden and that's all I am going to say."
The pair was seen ain the press box at Newsome's seat in the second row of the Ravens' front office seating area.
INACTIVES: The Browns inactives were OL Cosey Coleman, WR Brandon Rideau, CB Antonio Perkins, RB William Green, CB Michael Lehan, TE John Owens, and LB David McMillan. Derek Anderson was the third quarterback. Dave Yovanovits will make his first start of the season at RG in place of Coleman.


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PREDICT THE SCORE and scorers in a Fulham match and CHALLENGE FANS, friends and rivals across the world to WIN FULHAM PRIZES!
• It's FUN - tap away to predict the scores in the first game of it's kind!
• It's FREE - play through the whole Premier League season for free!
• It's FAMILY FRIENDLY - there is no betting or gambling, it's a game!
• It's REWARDING - earn XP to level up, earn coins to buy upgrades, win Fulham prizes!
• It's NEVER TOO LATE - every match is global tournament, no need to worry if you join after the start of the season!
The aim of the game is to make the most accurate prediction of the scoreline, scorer and score order. The more accurate you are, the more points you win, the higher you rank against Fulham fans around the globe. Play for fun against friends and family, play for power and prizes against rival fans or play for both at the same time!
Great app


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|Please note: This article is about the ronin. For other uses of the term, please see Nanashi (disambiguation).
Nanashi, which meant "no name", was a ronin who alongside Shinjo Fuyari founded the city of Nanashi Mura. [1]
Meeting Fuyari Edit
Fuyari was a corrupted samurai who had been dismissed from the Unicorn army after he made unwarrented attacks againsts the Dragon Clan and Crab Clan. Fuyari rallied a large army of ronin and ashigaru to attack the Unicorn, but when he was on the march, he ran into a large force of ronin led by a hooded figure. The hooded figure referred to himself as Nanashi and convinced Fuyari to stop his silly venture. [2]
Nanashi Mura Edit
Fuyari was accepted in the Dragon lands, and he set up Nanashi Mura on its southern parcel, named after the ronin that put him back on the path of honor. [3]
|This Ronin related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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I think that Combat Training is a very great feature of Black Ops. It entertains me a lot as opposed to having only 4 players in offline multiplayer in previous games. However I do have a few complaints:
- Hardened and Veteran difficulty are kind of unrealistic. One time a bot was walking forward, turned 180 degrees, and killed me with a headshot, under 3 seconds. This happens to me a lot, and I was wondering if it happened to anyone else.
- Bots getting headshots when they can't even see you.
- The bots' grenades are very accurate and can throw them at you, when you're behind walls, when they never even seen you behind the wall.
- Not many customizable features for offline multiplayer like in Modern Warfare 2, like no spawning or no Killcams. It would be really fun if you could do Gun Game or Hardcore Mode with the bots.
- Why only 10 people in one match unlike 12 people in Xbox Live Combat Training? Isn't fun doing 1 vs 11?
- No Combat Training in System Link.
Anyone else having complaints or suggestions?


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If you would like to ship your items to a different country, please select it below. Item availability, prices and shipping will be updated to reflect that country.
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Handy Binder
By Paul Abraham Jaimovich
Open iTunes to buy and download apps.
"Hard to believe there isn't an application as complete as this one in the store!"
We've all seen the notes applications out there… they are all missing a key element…that is why we gathered everything you might need when taking notes into one easy to use application.
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Small Bug Fix
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- Category: Utilities
- Updated: 20 December 2011
- Version: 1.1
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- Language: English
- Developer: Paul Abraham Jaimovich
Compatibility: Requires iOS 5.0 or later. Compatible with iPad.


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The company in question is, Fiserv, Inc. (NASDAQ:FISV) currently with a stock price of 99.3 (0.09% today). The market cap for Fiserv, Inc. is 21986.01, and is in the sector Services, and Business Services industry. The target price for Fiserv, Inc. is 108.36. Currently Fiserv, Inc. is trading with a P/E of 25.16, and a forward P/E of 19.95. Average volume for Fiserv, Inc. is 995.39 and so far today it is 706600.
Performance in the last year for Fiserv, Inc. has been 14.76%. For EPS growth, Fiserv, Inc. has seen a growth of 0.30%, and is looking to grow in the next year to 12.24%. More long term stats show that EPS growth has been 12.40% over the last five years and could be 12.50% for the next five years. Fiserv, Inc. has seen sales growth quarter over quarter at 5.00%, with EPS growth quarter over quarter at 77.30%. The 20-day simple moving average is -5.85%, with the 200-day simple moving average coming to -0.92%.
Since the IPO date for Fiserv, Inc. on the 3/26/1990, Fiserv, Inc. has seen performance year to date to be 8.57%. With Fiserv, Inc. trading at 99.3, the dividend yield is *TBA, and the EPS is 3.95.
So could Fiserv, Inc., be undervalued? Well as said before P/E is 25.16. The PEG is 2.01, P/S is 4.09 and the P/B is at 8.34. The P/cash is 83.6, with P/free cash flow at 19.42.
Fiserv, Inc. ability to deal with debt shows that the current ratio is 1, and the quick ratio is 1. This is with long term debt/equity at 1.72, and total debt/equity at 1.72.
In terms of margins, Fiserv, Inc. has a gross margin of 45.40%, an operating margin of 25.50% and a profit margin of 16.90%.Payout ratio for Fiserv, Inc. is 0.00%. Return on assets come to 9.60% with return on investment coming to 12.20%.
Insider ownership for Fiserv, Inc., is at 0.30% and institutional ownership comes to 89.40%. Outstanding shares are at 221.41. While shares float is 219.04. The float short is currently 1.59%, and short ratio is 3.5.


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While Siri is great for its clever quips, helpful suggestions and multi-functional abilities, you will soon be able to hold regular conversations with it and both of you may even joke around a bit.
Striving to improve Siri's communication skills, Apple has bought VocalIQ, a British 'speech tech AI specialist' for an amount ranging between £33 million to £66 million.
VocalIQ aims to let you share natural conversations with speech assistants by developing routines and not limit conversations to mere commands. This way, the virtual assistant will learn from one conversation and will apply the learning to future conversations, getting better and better every day.
"Previous conversations are central to its [the smart dialogue engine] learning process – allowing the system to better understand future requests and in turn, react more intelligently," says the firm.
Apart from learning how to interact, VocalIQ can also learn to recognize noise during conversations, thus separating background noise from actual noise accompanying a conversation.
Sounds exciting, doesn't it? So the next time you ask Siri a question about its origin, you'll get a proper answer rather than the usual 'Who me?' quip. With eleven months to go before Apple's next launch event takes place, we're sure that Siri in iOS 10 and in iPhone 7 will be a lot different from the one we're used to.
Back in April last year, Apple had acquired British tech firm Novauris Technologies with a view to improve Siri's capabilities, especially for offline usage.


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Elk Fest
September 30 - Oct 1, 2017
The haunting call of the bull elk fills the Estes Valley during the early days of fall. The call begins with deep, resonant tones that rise rapidly to a high-pitched squeal before dropping to a series of grunts.
What is this strange sound and what does it mean?
To celebrate the annual elk rut and learn about the "wapiti," the Native American name for elk, Estes Park hosts the 18th Annual Elk Fest in Bond Park and the surrounding area.
The free festival features bugling contests, Cabela's Archery Range, elk exhibits, the Rocky Mountain Raptor Program, elk seminars, elk-inspired arts and crafts, Native American music, dancing and storytelling, self guided elk tour maps, a children's' area with elk-themed activities, and a craft beer garden. For safe & respectful elk viewing tips, please visit our Wildlife Watching page.
Vendors will display artwork, handmade elk-ivory jewelry, and will offer distinctive elk cuisine. Mountain men from around the country will gather at the Mountain Man Rendezvous to sell their wares and demonstrate their skills.
Enjoy the Educational Seminar Tent to learn more about Elk and wildlife in Estes Park from a variety of professionals including Colorado Parks and Wildlife and Mile High Note Game Calls. Representatives from the Rocky Mountain Raptor Program will display on a gloved hand some of their magnificent rescued birds. It's amazing to see these majestic birds close up! Colorado Parks and Wildlife are on-site offering an Elk Camp where you can explore a variety of educational booths, and their staff will be helping in the field as attendees take the self-guided elk tour!
Live country, rock and bluegrass music will light up the park Saturday and Sunday with popular Colorado bands performing on stage.
Celebrate elk fest by participating in the 2nd annual Rut Run 5k on Saturday! Race day registration starts at 8:30am at Town Hall and the race begins at 9am. Participants will start and finish at Bond Park with an elevation gain of 246 feet and will receive a long sleeve shirt, a one pint beer token and burrito. Online registration coming soon!
On both Saturday and Sunday, stop into Town Hall (adjacent to Bond Park/Elk Fest) to watch a 20 minute video produced by the Estes Park News that shares the history of Elk in Estes Park and how elk adapt to each season in our mountain valley. Learn safety tips for elk watching and see footage of Estes Park elk that has been included in National Geographic, the BBC and many others have used in their worldwide programming. Stick around after with staff from Colorado Parks & Wildlife for Q & A.
On Sunday from 1-3pm, amateurs compete to see who can sound most like an elk bugling at the Elk Bugling Contest. Rules & registration information coming soon.
Schedule of Events
Saturday, September 30, 2017
8:30am: Rut Run - 5k
10am: All Vendors Open
10am-3pm: Kids Corral - Activities and Crafts
11am-12:30pm: Live Music - Ran Off the Rooster
11am-4pm: Elk of Estes Park - 20 min film in Town Hall
12:30-1:30pm: Native American Storytelling
1:30-3pm: Live Music - Wendhaven
3-5pm: Native American Music, Dancing & Storytelling
Sunday, October 31, 2017
10am: All Vendors Open
10am-3pm: Kids Corral - Activities and Crafts
11am-12:30pm: Live Music - Dahlby & Nadine
11am-3pm: Elk of Estes Park - 20 min film in Town Hall
12:30pm: Bugling Contest
1:30-3pm: Live Music - Wendhaven
3-4pm: Native American Music, Dancing & Storytelling
Vendor & Sponsor Information
If you are interested in participating in Elk Fest as a vendor or sponsor, please visit the Town of Estes Park website for details.


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On a remote Scottish island in the '50s, minister Balor (Damian Lewis) takes out his frustrations on his wife Aislin (Andrea Riseborough). When a juvenile offender, Fionn (Ross Anderson) is sent to live with them, tensions rise.
Andrea Riseborough is magnificent in this eerie, beautifully shot drama.
Andrea Riseborough is magnificent in this eerie, beautifully shot drama that's enhanced by her sensitive turn as the sad, distant wife of Lewis'bible bashing minister. The few residents of the island are beginning to pack up their belongings and head to the mainland in search of work, leaving the passionately pious Balor bereft and angry. Seeking inspiration from the Lord, he makes increasingly irrational decisions while his quiet, pale wife tries to evade his wrath.
Even though Aislin barely speaks for much of the first act, the script, direction and performance signal her deep unhappiness and sense of longing.
Aislin's world brightens considerably when Fionn is sent to live with them. He's a bookish, polite hard-grafter who's dismissed by Balor on the grounds of his criminal past - but who awakens a complex mix of maternal feelings, sensuality and creativity in the preacher's wife.
What begins as a blood-boiling portrait of an abusive husband hiding behind his religion becomes a story of a hesitant connection between strangers brought together through circumstance. Anderson is strong, and while Lewis overplays it with a shouty, fire and brimstone turn, Riseborough's soulful turn holds the interest and helps build erotic tension. Unanswered questions abound but it's fun trying to figure out the answers in this impressive feature from Corrina McFarlane (Three Miles North Of Molkom).
Riseborough steals the show in a moody period drama loaded with atmosphere and tension


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N1132 Ethnic Old Afgani Lapis Low Alloy Silver Necklace 23" by Eksha Creations
- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Ethnic, Folk & Native American Art
- Source: eBay
- Sold Date: Mar 23, 2013
- Channel: Auction House Sell similar item on eBay
N1132 Ethnic Old Afgani Lapis Low Alloy Silver Necklace 23" by Eksha Creations
This old ethnic necklace is made of afgani lapis, yellow resin and white metal beads which is also known as low alloy silver. This necklace is acquired from Kathmandu, Nepal. This necklace is 23 inches long and weighs 157gms (5.5oz). The approximate sizes of the beads measures from 10x9mm to 18x22mm. The size of the pendant measures 31x41mm.


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Tykes add Steele
West Brom goalkeeper Luke Steele has moved to Barnsley on an emergency loan.
Last Updated: 14/02/08 7:39pm
West Brom goalkeeper Luke Steele has moved to Barnsley on an emergency loan.
The 23-year-old will make a debut to remember for The Tykes, as he is set to start in Saturday's FA Cup clash with Liverpool at Anfield.
Barnsley have been forced to raid the transfer market as first choice keeper, Heinz Muller, has been ruled out for the rest of the season after rupturing his cruciate knee ligament.
His replacement Tony Warner, on loan from Fulham, is cup-tied.
In other news, Barnsley have won their appeal against Lewin Nyatanga's dismissal at Plymouth on Tuesday night.
The central defender was sent off at Home Park in the fourth minute for a professional foul on Jermaine Easter, but he will not now serve a suspension.