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|Mumbai, India
The Bombay High Court on Friday set aside the death penalty handed down by a session's court to Ramchandra Karanjule, the former director of an orphanage in Navi Mumbai, for murdering an inmate and gang-raping five mentally challenged girls inside the premises of the orphanage.
A division bench of justices RV More and Anuja Prabhudessai, while acquitting him of the charge of murder, commuted the sentence of death to a sentence of 10 years' imprisonment for the charge of gang-rape. Karanjule, who was produced in court escorted by the police, hugged his family members, who were present inside the court room, after the pronouncement of the sentence. The bench will be releasing a detailed copy of the judgment on Wednesday.
Karanjule, a former railway employee, had founded the Kalyani Mahila and Balak Seva Sanstha, which was located at Kalamboli.
The session's court had in 2013 acquitted four and convicted six of the 10 accused in the case. Of them, two were sentenced to life imprisonment. The convicted accused included Nanabhau Karanjule, Khandu Kasbe (both acquaintances of Ramchandra Karanjule), Sonali Badade (orphanage superintendent) and Parvati Mavale (its caretaker).
The high court, while reducing the life sentence imposed on Kasbe, sentenced him to 10 years' imprisonment on gang-rape charges, while Prakash Khadke, sentenced to life imprisonment, was acquitted of all charges. Apart from them, the court convicted Nana Karanjule, a freelance journalist, under charges of molestation and sentenced him to two years' imprisonment.
The court also sentenced Badade to one year in jail along with a fine of Rs2,000, acquitting her of the serious charge of attempt to murder but convicting her on the lesser charge of causing hurt. Mavale was convicted on the charge of causing hurt under section 324 of the IPC and sentenced to one year in jail with a Rs2,000 fine.
Karanjule, who has been in prison since his arrest in 2011, has undergone a substantial period of the 10-year sentence and could be released from prison after serving a few more years. The incidents of the sexual and physical abuse of the girls, aged 14 to 18 years, came to light during an inspection of 25 homes for differently-abled children under the instructions of the Bombay High Court.
According to defence lawyers Niranjan Mundargi and Mahesh Vaswani, the lower court's order was perverse and without an application of the mind.
The lower court, while sending Karanjule to the gallows, had observed: "The only objective behind running the orphanage was to satisfy lust and earn money. Ramchandra Karanjule not only exploited the girls for years but also allowed his friends to exploit the girls. He is a menace to society and life imprisonment will be highly inadequate in this case."
The prosecution's case was that 19 girls were allegedly gang-raped by three of the accused. The statements of the 19 victims were recorded by a magistrate and, of them; three had appeared before the court to testify against the accused and identified Karanjule, whom they referred to as 'Papa'.
The charge of murder was invoked against Karanjule after it came to light that one of the victims was suffering from jaundice and pneumonia when she was gang-raped. The victim subsequently died.
Source: DNA India, March 12, 2016
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ACU Workforce Framework
The ACU Strategic Plan 2015-2020 forms the basis for series of Enabling Frameworks, including the Workforce Framework, which guide the design and implementation of workplace initiatives and people management practices across the University.
ACU uses workforce profiling to inform the development of appropriate strategic interventions. We seek input from staff on their University through consultation processes and through the regular myVoice staff engagement survey.
Workforce Profiling
Strategic planning for aligning our staff profile to our strategic objectives.


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|Genetic Monster
|130 meters
|100,000 metric tons
|Created by
|Redesign by
|Matt Frank
We as a race are a remarkable evolutionary milestone, in that we humans are capable of molding our environment to suit our needs, and also constantly be creating new and improved methods to conquer and control the world around us. This progress comes at a price, however, as with new technology comes the danger of the unpredictable and the unknown. BIOLLANTE is such an unknown, and of such an unpredictability that she is beyond our present understanding of just what man has wrought.
Created from an amalgamation of Godzilla's Organizer G1, a rose's DNA, the blood of a kind-hearted girl, and the desperation of her mourning father, the myriad of influences lead to such a beast as Biollante. Initially, Dr. Shiragami was brought before G-Force with the hope of using Godzilla's DNA to create the Anti-Nuclear Energy Bacteria with hopes of defeating him once and for all...but Shiragami's disheveled mind had other plans. Seeking to keep some part of his late daughter alive, he had already mixed her DNA with that of her favorite flower; yet when a simple accident threatened to take her away from him yet again, he took the job only so that he could keep her cells alive forever... The ambition of a madman, tempered only by his undieing love. Biollante's psyche is apparently a coin-toss: one side is the gentle spirit of Erika Shiragami, who seeks to protect her father and bring serenity to the world, the other is an instinctual beast, who lashes out at a cruel and unsympathetic reality. First appearing as a colossal rose, she called out to sympathetic spirits, be they anywhere in the world, Erika herself desperate and confused in her newly reincarnated form. Of course...the only creature to respond was Godzilla himself. Seeking out this new creature, so like himself, Godzilla was agog at this giant mutation. Erika's mind went into overdrive, and the instinct to attack kicked in. Godzilla defeated her in this form, however...but she returned later, in a new and further evolved form, now more Godzilla than Erika. Biollante and Godzilla fought to a standstill, and the giant saurian retreated due to being weakened by the ANEB, whereas the plant monster, burned and wounded by Godzilla's raw power, dissolved into the most amazing cloud of ethereal flowers and glowing life-force, Erika's smiling face the last thing seen by Shiragami....before....
- - Able to secrete deadly radioactive sap with horrific corrosive ability.
- - Regenerative power equal to Godzilla's
- - Able to grow and replicate mouthed tendrils and spear-tendrils


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Classic Shrimp Salad with Thousand Island Dressing
This classic Classic Shrimp Salad with Thousand Island Dressing is loaded with bay shrimp, hard-boiled eggs, avocado, black olives and topped with a homemade dressing.
I love spring and summer. I also love sitting on our back patio and reading, no matter what the weather. Right now we have gorgeous sunshine in Oregon, but there will be plenty of rain ahead. April showers bring May flowers! (My front yard tulips.)
Grace, grace, and more grace
I've been reading a lot about grace in our lives, the grace we've been given as we age. Have you ever noticed how grace-filled women become more beautiful as they age, not less? Of course this has a spiritual component to it. If we allow God to shape us and work in our lives, we naturally mature as we grow in Him, reflecting a radiant inner beauty that can touch those around us when, we don't even know it.
I've been touched by those kinds of women. They've surrounded my life, some for 50 years, and when you're with them, it's always inspiring and encouraging. I hope to be one of those women, years from now.
I so love this …
Grace is something you can never get, but only be given. The grace of God means something like: Here is your life. You might never have been, but you are, because the party wouldn't have been complete without you. Here is the world. Beautiful and terrible things will happen. Don't be afraid. I am with you. Nothing can ever separate us. It's for you I created the universe. I love you. There's only one catch. Like any other gift, the gift of grace can be yours only if you reach out and take it. Maybe being able to reach and take it is a gift too. – Frederick Buechner
My author friend (I don't really know her in real life, but I love her books, so I consider her my friend), also shares: Grace is when the silence is so complete that you can hear your own heartbeat, and right within your ribs, God's beating heart, too. – Shauna Niequist
For me, to sum up grace, it's an unconditional, unmerited love that is renewed daily from my sometimes stubborn, ugly, and undeserving heart.
I'm thankful for a second chance, every day, to renew the freshness of what I've been offered in this life.
His mercies are new every morning. Great is His faithfulness!
Classic Shrimp Salad with Thousand Island Dressing
Have I told you how much I adore baby shrimp? Where I live it's called Oregon Pink Shrimp.
My Aunt Lillian would make a Shrimp Salad for every family dinner, traditionally for Easter, Christmas, or Thanksgiving. It was her signature dish, along with her fabulous homemade dressing, which I really believe "made" the salad.
Today I'm sharing that recipe, which is perfect for an upcoming Mother's Day, or springtime celebration, don't you think?
In my mind, there' s nothing quite like a fresh shrimp salad, with homemade Thousand Island dressing!
And yes, if you're wondering about the relish, this ingredient is a must, and it MUST be sweet relish, not dill. So yummy!
Classic Shrimp Salad with Thousand Island Dressing
Yield: 4-8
Prep Time: 20 minutes
Total Time: 20 minutes
You can store covered in the refrigerator for up to 2 days.
- 1 clove garlic, minced
- 1/4 tsp. garlic salt
- 3/4 cup mayonnaise
- 1/4 cup chili sauce
- 2 Tbsp. ketchup
- 1 1/2 Tbsp. minced red onion
- 2 teaspoons sweet pickle relish
- Salt and pepper to taste
- Lemon wedges
- 1 lb salad (baby) shrimp, washed and drained
- Iceberg lettuce, shredded (or your favorite salad greens)
- Tomatoes (optional)
- 4 hard-boiled eggs
- Shredded carrots
- Avocado, sliced
- Red onion, thinly sliced
- Black olives, drained
- Lemons for garnish
- For the dressing: In a small mixing bowl, whisk together the garlic, mayonnaise, chili sauce, ketchup, onion, and relish until combined. Season with salt and pepper to taste. Cover and let sit for about 30 minutes so the flavors blend. (You can store covered in the refrigerator for up to 2 days.)
- Arrange the salad ingredients decoratively on a low-line platter or bowl. Serve with the chilled dressing on the side, with extra lemon wedges.
More shrimp recipes:
Green Salad with Shrimp and Avocado (this recipe was from my old neighbor, Jean Lust)
Pineapple and Baby Shrimp Farro with Fried Rice
3 comments
Thank you Sandi for this reminder of His grace for all who believe. "Morning by morning new mercies I see. All I have needed they hand hath provided, Great is thy faithfulness Lord unto me." I am so thankful and look forward to reading your blog every day. It encourages me to be a better woman, full of God's love for others! Blessings!
Auntie LaLa's shrimp salad was the best! Thanks for the recipe to pass down.
Perfect spring salad, looks amazing!
Leave a Reply
Thank you Sandi for this reminder of His grace for all who believe. "Morning by morning new mercies I see. All I have needed they hand hath provided, Great is thy faithfulness Lord unto me." I am so thankful and look forward to reading your blog every day. It encourages me to be a better woman, full of God's love for others! Blessings!
Auntie LaLa's shrimp salad was the best! Thanks for the recipe to pass down.
Perfect spring salad, looks amazing!


text:
The rules of gene expression in plants: Organ identity and gene body methylation are key factors for regulation of gene expression in
Microarray technology is a widely used approach for monitoring genome-wide gene expression. For Arabidopsis, there are over 1,800 microarray hybridizations representing many different experimental conditions on Affymetrix™ ATH1 gene chips alone. This huge amount of data offers a unique opportunity to infer the principles that govern the regulation of gene expression in plants.
We used bioinformatics methods to analyze publicly available data obtained using the ATH1 chip from Affymetrix. A total of 1887 ATH1 hybridizations were normalized and filtered to eliminate low-quality hybridizations. We classified and compared control and treatment hybridizations and determined differential gene expression. The largest differences in gene expression were observed when comparing samples obtained from different organs. On average, ten-fold more genes were differentially expressed between organs as compared to any other experimental variable. We defined "gene responsiveness" as the number of comparisons in which a gene changed its expression significantly. We defined genes with the highest and lowest responsiveness levels as hypervariable and housekeeping genes, respectively. Remarkably, housekeeping genes were best distinguished from hypervariable genes by differences in methylation status in their transcribed regions. Moreover, methylation in the transcribed region was inversely correlated (R2 = 0.8) with gene responsiveness on a genome-wide scale. We provide an example of this negative relationship using genes encoding TCA cycle enzymes, by contrasting their regulatory responsiveness to nitrate and methylation status in their transcribed regions.
Our results indicate that the Arabidopsis transcriptome is largely established during development and is comparatively stable when faced with external perturbations. We suggest a novel functional role for DNA methylation in the transcribed region as a key determinant capable of restraining the capacity of a gene to respond to internal/external cues. Our findings suggest a prominent role for epigenetic mechanisms in the regulation of gene expression in plants.
Understanding the regulation of gene expression is essential to understand the form and function of living systems. Microarray technology has been widely used in many organisms to understand genome-wide changes in gene expression in response to treatments
To learn the general rules that govern gene expression in plants, we took advantage of a large microarray database available for Arabidopsis in the NASCarrays database
Results and discussion
The Arabidopsis transcriptome is robust to most perturbations but strongly influenced by organ type
In an effort to discover new principles that govern gene expression in
Additional File 1
Control vs. tests comparisons. List of the analyzed 474 comparisons in the NASCarrays database, annotated according to the experimental factor and plant structure categories. NASC experiment numbers are provided.
Click here for file
Figure 1
Classification of experiments from the NASCarrays database
Classification of experiments from the NASCarrays database. Pie charts with the classification of microarray experiments according to the experimental factor categories defined by TAIR (A) or the organ used to extract RNA to perform the microarray experiments (B).
We first examined the number of differentially regulated genes per comparison. We found their distribution to be far from normal. As shown in Figure
Additional File 2
Gene responsiveness by categories. Table detailing the number of experiments, within the eight experimental categories, in which each Arabidopsis gene is regulated. The number in parenthesis in the header of the Table indicates the total number of experiments in each category.
Click here for file
Additional file 3
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 4
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 5
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 6
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 7
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 8
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 9
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Additional file 10
Genes regulated specifically in one experimental category. Each file provides the individual genes responding exclusively in abiotic, biotic, ecotype, chemical, hormone, mutant, nutrient or organ comparisons, respectively.
Click here for file
Figure 2
Global characteristics of the Arabidopsis transcriptome
Global characteristics of the Arabidopsis transcriptome. A) Histogram of the number of genes (X-axis) regulated in a given number of comparisons (Y-axis). B) Average number of genes regulated by each experimental category as defined in Figure 1A. C) Histogram of the number of comparisons (X-axis) for which the specified number of genes (Y-axis) show significant regulation.
Given its impact on global gene expression levels, we next wished to evaluate the importance of organ type in the context of typical experimental factors that are tested in the laboratory. We compared the number of genes responding in shoots or roots for each of the nine treatments in the AtGenExpress abiotic stress series. On average, only 13% of the total genes that responded to a treatment responded in both organs. By contrast, a much higher proportion of genes (88%) were regulated by the treatment in an organ-specific manner (Additional File
Additional File 11
Importance of organ type in the response to abiotic stress in Arabidopsis. Percentage of genes responding to various stresses in either roots, shoots or both. Data corresponds to the AtGenExpress Abiotic Stress series present in the NASCarrays database. The black zone indicates the percentage of genes responding only in roots; the white zone indicates those responding only in shoots, and the black squares region indicates the genes responding in both tissues
Click here for file
Housekeeping and hypervariable genes possess marked structural differences
To identify properties that explain the capacity of a gene to respond to stimuli, we ranked genes based on the number of comparisons in which they are differentially expressed. As shown in Figure
Additional file 12
Gene responsiveness. Gene responsiveness as determined by the Rank Products and fold-change method.
Click here for file
Additional file 13
Housekeeping and hypervariable genes and their methylation status (1). List of Housekeeping and hypervariable genes, classified according their methylation status as defined in:
Click here for file
Additional file 14
Housekeeping and hypervariable genes and their methylation status (2). List of Housekeeping and hypervariable genes, classified according their methylation status as defined in:
Click here for file
A previous study positively correlated expression levels with gene size in plants
Additional file 15
Function of housekeeping and hypervariable genes. Analysis of over-representation of gene ontology functional terms in housekeeping and hypervariable genes (performed in VirtualPlant –
Click here for file
Table 1
Contrasting features of housekeeping and hypervariable genes.
Gene feature
CDS length (bp)
2624 (s.e. = 89)
1178 (s.e. = 73)
1931 (s.e. = 8)
Gene length (bp)
3117 (s.e. = 87)
1493 (s.e. = 78)
2229 (s.e. = 8)
Total exon length (bp)
1941 (s.e. = 52)
1169 (s.e. = 50)
1568 (s.e. 6)
Total intron length (bp)
1173 (s.e. = 52)
323 (s.e. = 44)
660 (s.e. = 4)
Number of exons (pb)
8 (s.e. = 0.31)
3 (s.e. = 0.24)
5 (s.e = 0.03)
Genes without introns
6% (p = 5E-16)
33% (p = 0.0007)
Average number of transcription factor binding sites
27 ± 1.2 (p < 0.01)
46 ± 1.8 (p < 0.0001)
30 ± 0.1
TATA-containing genes
5% (p = 1.3E-6)
45% (p = 6.1E-15)
Genes coding for unstable transcripts
0% (n.a.)
8% (p = 9E-11)
Shared among eukaryotes
18% (p = 0.002)
34% (p = 2E-10)
Body methylation
63% (p = 1.5E-35)
8% (p = 2E-10)
Promoter methylation
Body methylation
36% (p = 9.1E-21)
2% (p = 3.8E-8)
The first column lists various features analyzed for housekeeping genes (second column), hypervariable genes (third column) and the whole genome (fourth column). Rows report average and standard error or percentage values. P values for significant (p < 0.01) enrichment or depletion as compared to the genome occurrence are shown in parenthesis.
Eukaryotic genes are transcriptionally regulated by the coordinated interaction of multiple protein factors that interact with discrete binding sites and with each other
Additional file 16
Enrichment of cis-acting motifs in the promoter of hypervariable genes. Frequency distribution of the number of predicted transcription binding sites in the promoter of housekeeping and hypervariable genes and the whole genome. The genes were ranked according to the number of cis-acting regulatory elements in their promoters according to the AGRIS database (X-axis). The points represent the fraction of genes in a bin of 10 motifs.
Click here for file
In addition to gene structure, epigenetic mechanisms such as DNA methylation are known to have an impact on gene expression in eukaryotes, particularly in heterochromatic regions
Transcript region methylation is the most important factor to explain genome-wide responses to internal/external stimuli
To evaluate the importance of these features for gene expression responses on a genomic scale, we performed a regression analysis of the gene responsiveness for all Arabidopsis genes as a function of each of the structural features described above. We used a linear model of the form: Y ~ αX + β, where Y was the observed gene responsiveness of all genes and X was the structural feature under evaluation (e.g. presence of TATA-box, cis-acting binding sites in the promoter or gene body methylation). Thus, the effects detected were free from any bias arising from gene selection, as could be the case when analyzing this relatively small group of housekeeping and hypervariable genes.
Notably, using the two independently generated methylome datasets
Additional file 17
Correlation between gene responsiveness as determined by the fold-change method and gene body methylation. Table listing gene responsiveness as determined by the fold-change method (≥ |2|), and the corresponding frequencies of methylated genes.
Click here for file
Additional file 18
Plot of the correlation between gene responsiveness determined by the fod-change method versus gene body methylation. This graphs shows the linear correlation between gene responsiveness as determined by fold change ((≥ |2|) and gene body methylation.
Click here for file
Additional file 19
Results of simple regression models, given by experimental category. Description is as Table
Click here for file
Additional file 20
ANOVA models for the effect of methylation and TATA-box presence on gene responsiveness, by category of experimental treatment. The models have the form Y ~ aX + b, where X was a factor encoding the presence or absence of those features as two different levels. We used the 'aov' function in R to fit the model. The F statistic estimates the significance of the contribution of the factors to the response. The differences between the levels of the factors were estimated by the Tukey procedure, using the 'glht' function from the 'multcomp' package in R. This is equivalent to comparing the coefficients of the factors. The Bayesian Information Criteria was calculated in R using the 'BIC' function in the package 'nlme'. This parameter represents the "a posteriori" probability of the model to be true, being maximized when the magnitude of the parameter is minimized.
Click here for file
Table 2
Results of the simple and multiple linear regression analyses
Explanatory variable(s)
Data Source
r 2
Methylation frequency
Frequency of genes target of H3k27me3
Gene size
TAIR Genome v6.0
TATA-box frequency
Methylation + TATA-box
Column 1 reports the explanatory variables used to model gene responsiveness. Column 2 indicates the source of the data (reference). Columns 3 and 4 report the different statistics obtained with the linear regression. n.r., not reported; n.d., not determined.
Figure 3
Correlation between methylation and gene responsiveness
Correlation between methylation and gene responsiveness. (A) Plot of the frequency of methylated genes (according to Zhang et al.
The effect of DNA methylation on gene responsiveness could be explained by a simple transcriptional gene silencing effect
Additional file 21
Lack of linear correlation between expression levels and gene responsiveness. Box plot of the signal of a gene across the whole NASC arrays dataset (X-axis) versus gene responsiveness (the number of comparisons in which it is significantly regulated, Y-axis). A simple linear regression model cannot explain the variability in the data (R2 = 0.04).
Click here for file
Figure 4
Lack of linear correlation between expression levels and gene body methylation or TATA-box presence
Lack of linear correlation between expression levels and gene body methylation or TATA-box presence. (A) Plot of the median expression level across the whole NASC arrays dataset in 10% bins (X-axis) versus the frequency of methylated genes in the bin (Y-axis), as determined by Zhang et al.
We also evaluated the relationship between the presence of modified histones and gene responsiveness. We used a recently published genomic survey of trimethylation in lysine 27 of histone H3 (H3K27me3) f
Gene body methylation and regulation of expression by nitrate in TCA cycle genes
As a case-study and to provide a concrete example of the influence of methylation patterns on the regulation of gene expression, we focused on a discrete biological process and experimental factor: nitrate. Nitrate has been shown to be a signal to regulate gene expression in plants
Table 3
Relationship between the methylation status and nitrate regulation of TCA cycle genes.
AGI number
Gene Annotation
Responsiveness to nitrate
Methylation statusa
MDH (malate dehydrogenase); malate dehydrogenase
malate dehydrogenase, cytosolic, putative
malate dehydrogenase (NAD), mitochondrial
PMDH1 (PEROXISOMAL NAD-MALATE DEHYDROGENASE 1); malate dehydrogenase
malate dehydrogenase (NAD), mitochondrial, putative
PMDH2 (PEROXISOMAL NAD-MALATE DEHYDROGENASE 2), PMDH2 (PEROXISOMAL NAD-MALATE DEHYDROGENASE 2); malate dehydrogenase
malate dehydrogenase, cytosolic, putative
malate dehydrogenase (NADP), chloroplast, putative
malate dehydrogenase, cytosolic, putative
isocitrate dehydrogenase, putative/NAD+ isocitrate dehydrogenase, putative
IDH1 (ISOCITRATE DEHYDROGENASE 1); isocitrate dehydrogenase (NAD+)
isocitrate dehydrogenase, putative/NADP+ isocitrate dehydrogenase, putative
isocitrate dehydrogenase, putative/NAD+ isocitrate dehydrogenase, putative
isocitrate dehydrogenase, putative/NAD+ isocitrate dehydrogenase, putative
isocitrate dehydrogenase, putative/NADP+ isocitrate dehydrogenase, putative
ICDH (ICDH); isocitrate dehydrogenase (NADP+)
This table provides the AGI number, the gene annotation, regulation by nitrate as determined from four independent experiments (see main text) and the methylation status according to the two methylome datasets used in this work. This table includes all the different malate dehydrogenase and isocitrate dehydrogenase isozyme-coding genes present in the Arabidopsis genome, according to VirtualPlant
The analysis of the large and heterogeneous whole-genome microarray dataset available in the public domain proved useful to evaluate principles that govern regulation of gene expression in plants. Our global and systematic analysis of the quantitative effect of different experimental factors (e.g., mutations, stress and organ identity) on the plant transcriptome revealed the key role of developmental processes for establishing mRNA levels throughout the plant. This process in turn determines how cells, organs and tissues respond to exogenous cues. Our data indicate that plant responses to external stimuli are strongly organ-dependent and underscore the need for a more thorough survey of organ-specific and, by extension, cell-specific responses in Arabidopsis and other plants
The second part of our analysis provided a weighted insight into the role of different molecular mechanisms in the global regulation of gene expression in Arabidopsis. The data indicate that DNA methylation within the body of Arabidopsis genes is a key factor that may determine or negatively influence the capacity of genes to respond to internal or external cues. The presence of a TATA-box may favor gene responsiveness but to a lesser extent than the negative effect of DNA methylation. Surprisingly, our data indicate that other gene structural features (e.g., number of cis-acting elements, gene size, presence and number of introns) are less important than DNA methylation and the presence of a TATA-box. These results highlight the importance of epigenetic mechanisms for the global control of gene expression. As a concrete example, we found consistency between regulation by an external stimulus (nitrate) and gene body methylation for a discrete biological process, the TCA cycle, beyond what would be expected by chance. The results presented here suggest a model whereby gene body DNA methylation restrains the ability of a gene to be regulated, regardless of regulatory signals (e.g., binding sites for specific transcription factors in the promoter region). This effect would not be directly dependent on basal gene expression levels. Moreover, our results provide a plausible functional role for the DNA methylation that is found in the body of a large number of Arabidopsis genes. This new role differs from the proposed role for DNA methylation in suppressing spurious transcriptional initiation
Data processing
The CEL data files comprising all ATH1 Affymetrix hybridizations through the end of 2005 were obtained from NASCArrays through the AffyWatch Subscription Service. This data comprised 1887 hybridizations corresponding to 108 different experiments. The entire hybridization set was normalized using the Robust Multiarray Analysis method
For the analysis of differential expression, the remaining 1701 hybridizations were mapped to their corresponding experiments. Controls and biologically meaningful tests were identified and grouped with their replicates. Comparisons in which the control or treatment hybridizations had less than 2 replicates were discarded. This process resulted in a list of 474 biologically meaningful comparisons (control versus test), including 1295 hybridizations. In the case of tissue comparisons, we used rosette leaves as a control, and all other tissues were considered tests. Rosette leaves were chosen as the reference because they are the prototypical organ system
Differential expression analysis
The comparisons were analyzed for differential gene expression using the RankProducts method
We evaluated the functional coherence of the differentially expressed genes by the different methods by evaluating enriched gene ontology (GO) terms in the resulting lists. For most of the comparisons tested, visual inspection revealed enriched GO terms that were obviously related to the experimental factor. This was not the case for the other methods. As an example, 245 genes were found to be differentially expressed in the comparison DO.1.1 (Additional File
With the results of the differential expression analysis, a "regulation matrix" was created. This matrix contained the p-value for the down- and up-regulation of all of the ATH1 Affymetrix chip probes across the 474 comparisons. The cut-off for defining a probe as differentially expressed was 0.05. The complete data file with ratios is available from
Housekeeping and hypervariable gene definition
The least responsive genes (housekeeping genes) were defined as follows: first, we selected genes which did not show differential expression in any comparison (5652 genes). Second, these genes were filtered for expression above the median of the entire NASC dataset (1758 genes). Third, we choose only those having a signal difference between the 1st and 3rd quartile (interquartile range) that was in the bottom 5 percentile of the signal interquartile ranges from the whole dataset. This ensured the selection of 384 expressed Arabidopsis genes that exhibit the lowest expression variability.
For the most responsive genes (hypervariable genes), we first choose genes that were regulated in 86 or more comparisons, corresponding to the top 1% most responsive genes from Figure
Structural and phylogenetic analyses and correlation with gene responsiveness
Gene structural features (gene, CDS, exon, intron lengths and numbers) – were obtained from the TAIR 6.0 Arabidopsis genome
Statistical and regression analysis
Calculation of significant enrichment or depletion was done in R using the hypergeometric distribution. t-tests were carried out with the GraphPad Prisma 4.0 software. Simple and multiple linear regression models used to predict gene responsiveness as a function of various structural parameters were done in R. We used simple models of the form:
Gene body methylation and regulation by nitrate for TCA cycle genes
We retrieved the genes corresponding to the TCA cycle from AraCyc
Authors' contributions
FFA carried out the bioinformatics and statistical analyses and wrote the manuscript. NM and SYR revised the manuscript critically for important intellectual content. RAG carried out some of the bioinformatics analyses, wrote the manuscript and was responsible for the conception of the study, the design of the data analysis and the interpretation of the results. All authors read and approved the final manuscript.
We thank Xiaoyu Zhang, Dr. Steve Jacobsen and Dr. Joseph Ecker for kindly providing genome-wide DNA methylation data in a custom format, and Juanita Larraín-Linton for her proof-reading. This work was funded by grants from: ICGEB (CRPCHI0501), FONDECYT (1060457), MILLENNIUM NUCLEUS FOR PLANT FUNCTIONAL GENOMICS (P006-09-F), FUNDACION ANDES (C14060/62) and NSF (DBI0445666) to R.A.G. F.F.A. was funded by a Ph.D. fellowship from CONICYT.
The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses
A gene expression map of Arabidopsis thaliana development
A Gene Expression Map of the Arabidopsis Root
Transcriptional profiling of the Arabidopsis embryo
Functional discovery via a compendium of expression profiles
A Gene Expression Map for Caenorhabditis elegans
A gene-coexpression network for global discovery of conserved genetic modules
Quantifying the relationship between co-expression, co-regulation and gene function
Orchestrated transcription of a key pathway in Arabidopsis by the circadian clock
NASCArrays: a repository for microarray data generated by NASC's transcriptomics service
Rank products: a simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experiments
Comparison and evaluation of methods for generating differentially expressed gene lists from microarray data
AP2 Gene Determines the Identity of Perianth Organs in Flowers of Arabidopsis thaliana
Comparative studies on the type-B response regulators revealing their distinctive properties in the His-to-Asp phosphorelay signal transduction of Arabidopsis thaliana
Cell-fate specification in the epidermis: a common patterning mechanism in the root and shoot
LEAFY controls floral meristem identity in Arabidopsis
In plants, highly expressed genes are the least compact
Phylogenetic profiling of the Arabidopsis thaliana proteome: what proteins distinguish plants from other organisms?
Identification of unstable transcripts in Arabidopsis by cDNA microarray analysis: rapid decay is associated with a group of touch- and specific clock-controlled genes
A Unified Theory of Gene Expression
The regulatory code for transcriptional response diversity and its relation to genome structural properties in Arabidopsis thaliana
Gardening the genome: DNA methylation in Arabidopsis thaliana
DNA methylation dynamics in plant genomes
Genome-wide high-resolution mapping and functional analysis of DNA methylation in arabidopsis
Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription
Genome wide analysis of Arabidopsis core promoters
PlantProm: a database of plant promoter sequences
Multiple comparisons
Estimating the dimension of a model
Whole-genome analysis of histone H3 lysine 27 trimethylation in Arabidopsis
Partitioning of the Maize Epigenome by the Number of Methyl Groups on Histone H3 Lysines 9 and 27
A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells
Histone Methylation-Dependent Mechanisms Impose Ligand Dependency for Gene Activation by Nuclear Receptors
Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis
Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen
Genome-wide patterns of carbon and nitrogen regulation of gene expression validate the combined carbon and nitrogen (CN)-signaling hypothesis in plants
Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1,000 rapidly responding genes and new linkages to glucose, trehalose-6-phosphate, iron, and sulfate metabolism
Steps towards an integrated view of nitrogen metabolism
CpG methylation is targeted to transcription units in an invertebrate genome
Exploration, normalization, and summaries of high density oligonucleotide array probe level data
Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets
The Arabidopsis Information Resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and community
RankProd: a bioconductor package for detecting differentially expressed genes in meta-analysis
Controlling the False Discovery Rate: a practical and powerful approach to multiple testing
Statistical significance for genomewide studies
An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach
AGRIS: Arabidopsis Gene Regulatory Information Server, an information resource of Arabidopsis cis-regulatory elements and transcription factors
AraCyc: A Biochemical Pathway Database for Arabidopsis


text: You can find the source code here. There is also a list of changes. If you are even worse at Perl than me you and you want to create something similar to this site, you may of course use the script as a starting point for your own experiments. The script is available under the GNU General Public License.


text: One affordable LG Android was spotted in January (LG Optimus 2) and now there's another – the LG Optimus L3 E400.Likely to be called simply the LG Optimus L3 when it finally takes an official bow, the phone has been spotted at a Swedish online retailer for the equivalent of £120. Which tells us that this is very much a budget phone and that it is intended for the European market. So expect it on the shelves of our very own retailers fairly soon.Its curved metallic frame and latticed back perhaps hint at a premium phone, but the features reflect that price point, with the L3 offering up a 3.2-inch screen (320x240 pixels) and a three-megapixel camera (without a flash), but it does come with Android 2.3 Gingerbread for the operating system. Colour choice for the phone is black or white.The retailer is talking 27 February for availability, so still time for a UK launch / UK availability before that date. If we get some official word, we'll let you know. Otherwise, look out for it as a minor MWC launch by the company.Source: Recombu


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The bathroom can be seen in these episodes:
- [S01E01] - Penny showers
- [S02E06] - Sheldon hides from Ramona Nowitzki
Sheldon has placed a UV sterilization light there to stop his toothbrush from being infected by germs. Sheldon also has tape on the floor which serves to avoid splatter on the mirror from flossing teeth, and to stop urine from getting on the floor when men use the toilet. Sheldon also has a squeegee to clean the mirror.
Other items seen in the bathroom include:
From the pilot episode
Other items:
- robot launching poster (moves to living room next episode)
- unidentified photograph of a pipe/valve framed over the towel cabinet
- cherry bookshelf with glass doors used as a towel cabinet*
- French Country style faucet by Kingston Brass, pewter finish
From S02E06 "The Cooper-Nowitzki Theorem"
|McCandles II space walk
|City of Heroes poster
|Gentle Scrub bath mitt by Aquasentials
|Hyrdo Body Sponge by Earth Therapeutics
|AVIVA Shower Dispenser
|Wicker Hamper
- Framed over the toilet, the astronaut image features NASA's Bruce McCandles II making the 1st untethered walk using the Manned Maneuvering Unit on February 7, 1984.
- A super rare City of Heroes poster is behind the door over the towel cabinet (it's an enlargement of the artwork from inside the CD)
Later episodes
- "Bounty Hunters" artwork by Randy Martinez. Limited edition of 350 prints. Seen above the towel cabinet. Reprints are now available from AcmeArchives .


text:
Flaire T-shirt (Lavender)
Flaire T-shirt (Lavender)
I wanted a T-shirt with a bit of flaire - and this is the result! I designed the bottom of the tee so that it hovers just over the belt and shows a bit of belly cos the girls got cute bellies. Made in Kyushu Japan.
- #For warranty info read this.
- #Folks making a purchase should read our Design Philosophy post first.
- #If you are purchasing a Smart Doll then make sure to read the Welcome Guide.


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Support the Press
Dear Friend—
The Ashland Poetry Press has grown tremendously in the ten years since we founded the annual Richard Snyder Publication Prize, awarded to the best book-length manuscript of poems submitted to the press. Of the ten books published in the series to date, two books placed in national competitions for prestigious book prizes. Vern Rutsala's The Moment's Equation was a finalist for the 2005 National Book Award. Another Snyder Prize winner, Christine Gelineau's Remorseless Loyalty, was nominated by David St. John for a 2006 Los Angeles Times Book Prize. Another book published outside of the Snyder series, Kathryn Winograd's Air into Breath, was co-winner of the 2003 Colorado Book Award in Poetry.
In 2004 the Ashland Poetry Press established a second book series, the Robert McGovern Publication Prize, awarded to poets over 40 years of age who have published no more than one book. Four books have appeared in the series to date, with a fifth book planned for 2008. Unlike the open competition of the Snyder Prize, manuscripts for the McGovern Prize are considered by nomination only. There is no submission fee. McGovern manuscripts are nominated by a panel of renowned American poets and editors, including Philip Levine, Alice Fulton, Vern Rutsala, Robert Phillips, Carolyn Forche, Annie Finch, William Heyen, Andrew Hudgins, Gregory Wolfe, Eamon Grennan, John Kinsella, Richard Jackson, and Gerry LaFemina.
APP has grown in other ways too. The founding of the Ashland University MFA in Creative Writing Program has allowed the English Department to hire a full-time administrative director who will also serve as Managing Editor of the poetry press. Our books are also now nationally distributed by Small Press Distribution and by Atlas Books. In ten years we have published 19 titles, with an additional book currently in production. The press has been fortunate to operate within Ashland University, receiving a whole range of non-monetary support along with a nominal annual stipend, and surviving financially through modest sales revenues, submission fees, and occasional grants.
We are now in a new era in which we are addressing the long-term needs of the press. One of our long-term goals is to establish an endowment to support the press far into the future. Since the press was founded in 1969, we have never previously approached friends of APP with a request for contributions. We hope your passion for the literary arts will motivate you to give. Please consider making a tax-deductible contribution to the Ashland Poetry Press so we may build upon a forty-year legacy of publishing beautiful books.
- Stephen Haven, Director
- Deborah Fleming, Editor


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Please enter the email address for your account. A verification code will be sent to you. Once you have received the verification code, you will be able to choose a new password for your account.
+351 262 380 267
Mint Lda, (Hey Portugal) Ave Eng Luis Paiva e Sousa, Loja 2C Nossa Senhora do Populo2500-329 Caldas da Rainha
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News By Tag
Industry News
News By Location
Industry News
Starving in South Sudan's Swamps: Families Eating Water Lilies to Survive
The UN reports that more than a third of South Sudan's population, or 4 million people, will be on the brink of starvation by the end of 2014; Medair's nutrition centres see massive increase in new admissions.
Initial assessments have shown shocking acute malnutrition rates of 31 percent in Panyijar County, a swampy area where more than 30,000 people fled to escape the violence, doubling the population. "The swamps create a very tangible barrier from the insecurity that exists on the other side," said Medair's Dr. Trina Helderman. "Sadly, this safe haven severely lacks food. Food was already insufficient for the local population due to floods last year, but now with almost double the population, food is scarce to non-existent. Families are surviving on leaves and water lilies. "
In Panyijar, the International Rescue Committee (IRC) asked Medair to work alongside them and provide life-saving treatment for children with moderate acute malnutrition and for the most severely malnourished children and pregnant/lactating women. "Seeing all of the malnourished children here and knowing that there is such a shortage of food is heartbreaking,"
"People have almost exhausted the water lilies that they eat," said Medair's Eunice Kavoi. "They have to go very far to get them now, which is also not safe." Indeed, the swamps are full of dangers: murky water that reaches neck-high depths, deadly predators such as crocodiles and snakes. Yet families who live hours away are braving the journey to reach Medair's feeding programme for the possibility of receiving life-saving care and food.
"We are from Pomalual, which is a four- hour walk, but there's no other clinic nearby," said young mother Martha Kong. "I had to walk through the swamp, which is very dangerous, but where else could I go? If I had stayed home, I know my child would have died from hunger."
Meanwhile, malnutrition cases have almost tripled since February in a camp of 40,000 Sudanese refugees in Maban, according to Medair health workers. "Children wait outside our nutrition centres to collect used wrappers from the nutrition supplement we distribute, so that they can lick the inside of them," said Dr. Lois Fergusson, Medair Health Manager.
Now that the rainy season is starting, which runs from May to October, supply routes already hampered by insecurity will be cut off by flooding, while wet conditions bring an increased risk of diseases that can be lethal in combination with malnutrition.
"We are working night and day," says Peter Manyang, a relief worker who helps run the centre in Panyijar where the most severe cases are treated. "Almost everyone is starving in Ganyliel villlage, big and small," says Eunice. "The majority of people fled their homes without blankets, without anything, when their villages were torched. When we first went in, there were hardly any signs of life."
Despite the scale and severity of the crisis, there is still hope. If media agencies can raise enough awareness and relief agencies can raise enough support, we know that food provisions and appropriate treatment will make an immediate life-saving difference for weakened families. "When the World Food Programme food drops started and Medair's nutrition centre opened, you could start to see some hope," says Eunice. "Children started playing again at the airstrip, we could hear laughing. Signs of life started to come back." ____________________________________________________________
Medair has been present in South Sudan since 1992, and since the conflict began in December has assisted thousands of affected people through nutrition, health, water and sanitation, and emergency shelter. Alongside its response to the nutrition crisis, its emergency response teams are also working to contain a recent cholera outbreak in Juba.
For media: please contact Abigail Woodcock, Press Relations Officer (English) abigail.woodcock@
Timothy Chapuis, Communications Manager (English, French) timothy.chapuis@
For inquiries and interviews on the ground, please contact Wendy van Amerongen, Communications Officer (Dutch, English) email@example.com, +211 (0)927 475 150
Recent photos of Medair's work in Ganyliel and other areas are available for use upon request. For more information on Medair's South Sudan programme, click here. (http://relief.medair.org/
Medair's South Sudan programme is supported by the EC Directorate-
Figures are taken from the United Nations Office for the Coordination of Humanitarian Affair's South Sudan SitRep No.36, 16 May 2014 and from UNICEF.
Medair helps people who are suffering in remote and devastated communities around the world survive crises, recover with dignity, and develop skills to build a better future.
Medair - Abigail Woodcock
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text:
Online Cartoon
Orçamento $1500-3000 USD
A series of short 5 minutes cartoons, for distribution on the internet
32 freelancers estão ofertando em média $2176 para este trabalho
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text:
This high quality audiophile headshell provides an elegant solution with a variety of cartridges
Standard P-mount replacement headshell. Replace your headshell, or keep a spare. Great for switching cartridges quickly.
Fits all standard mount tonearms.
OFC Gold plated cartridge leads
56mm in length
Metal finger lift
Attractive cherry wood body
Mounting screws not included
Weight 9.0grams
Item as described, I am very pleased with it, and also the quick delivery
Was this helpful to you? Yes / No
This review has not been appraised.
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text:
Banks provide billions for Dirty Diesel traders while failing to act on human rights, says new briefing
- Of 26 banks contacted, not one has pressured companies over toxic fuel exports to Africa
BankTrack is the international tracking, campaigning and support organisation focused on banks and the activities they finance.
We aim to promote fundamental changes in the banking sector so that banks adopt just and sustainable business practices.
Tianju Wang, Greenovation Hub
Yu Xiaogang, Green Watershed
Katharine Lu, Friends of the Earth US
ICBC (Industrial and Commercial Bank of China) is a joint-stock commercial bank based in the People's Republic of China. Founded in 1984, ICBC overtook Citi to become the world's largest bank by asset value in 2007, a position it retains in 2014. ICBC has expanded its operations outside of China through mergers and acquisitions, as well as registering branch offices in foreign countries. Its most important overseas strategic investor is Goldman Sachs. In 2007, ICBC acquired PT. Bank Indonesia and Heng Seng bank, as well as a 20% stake in Standard Bank of South Africa. It also set up offices in Russia, Dubai, Doha, Sidney and New York.
In 2015, the bank was named "Best Emerging Markets Banks" by Euromoney, and ranked 1st place among the Top 1000 World Banks by the Banker. As at the end of 2015, total assets of ICBC amounted to USD 3,300 billion and the total liabilities of the bank amounted to USD 3,052 billion.
No. 55 Fuxingmennei Avenue, Xicheng District
100032 Beijing
Mr. Jiang Jianqing
|Annual reports
Annual Report 2015
Corporate Social Responsibility Report 2015
listed on Hong Kong Stock Exchange & Shanghai Stock Exchange
The bank has declared in its CSR policy improvements listed below.
Three-year strategic goal of increasing green credit through developing green credit concepts, frameworks, and implementation steps.
In accordance with relevant national policies on environmental protection, resource conservation, and carbon emissions reduction, ICBC has formulated "Opinions on Strengthening of Green Credit Construction", "Views on Further Energy-saving and Emission-reducing Credit Support", "Classification and Management of the Domestic Corporate Green Credit Loans" and other rules and regulations; the bank also produced such environmental risk policies as "ICBC Post-Loan Management Approach Focusing on Environmental Protection by Industrial Customers" and "Notice on Further Strengthening of Loan Environmental Risk Prevention and Control Procedures".
The bank published the Implementation Outline for Green Credit Development. It clarified at the strategy level the basic aims and principles and key points for the implication of green credit development, and specified the direction and requirements for the building of the green credit system across the Bank in the future in terms of credit culture, classified management, policy system, process management, product and service innovation, evaluation mechanism and ability development.
According to relevant policies of the State concerning environmental protection, energy conservation and emission reduction, the Bank, has during the reporting period, successively made a series of policies, including the Notice on Optimizing and Improving the Green Credit Classification of Corporate Customers, the Notice on Printing and Distributing the Post- credit Management Procedure for Environmental Protection Industries under Key Attention, the Notice on Strengthening the Risk Control on Enterprises with Outdated Capacities, the Notice on Strict Control over the Credit Risk Associated with Small & Medium Lead-related Enterprises, the Notice on Strengthening the Prevention and Control of the Environmental Risk of Industries Involving Cadmium, Mercury, Chrome and Metalloid Arsenic and the Notice on Accelerating work in relation to Loan Exit from Small & Medium Lead Accumulator Enterprises. The Bank further improved the classification system for green credit, carried out and strengthened the monitoring of environmental risk, post- credit inspection, post-credit risk control requirements and post-credit management responsibilities with respect to customers in industries with a key focus on environmental protection, clarified the working mechanism and management requirements for risk control associated with enterprises with outdated capacities.
Amendment of its approach to "the disclosure and management of social responsibility information," in response to the developing trends of corporate citizenship and stakeholder demands.
Update of its "industrial (green) credit policies", which already cover 85 percent of corporate loans to(China's) 54 industries, and will put more emphasis on the main factors of industrial pollution, resource consumption, energy savings, and environmental protection,and integrate these with the core targets of the whole industrial (green) credit policies.
Publication of the "Notification regarding the increase of emission controls of heavy metal industries' commercial loan credit risk management," which takes the credit risk management for heavy metal industries one step further and specifies the involvement of heavy industry emissions in the scope of key industries.
The Bank established correlation between green credit classification and enterprise ratings, and transplanted the correlation to the credit process. It is required to timely identify the green credit classification label of customers throughout the procedure of due diligence, credit rating and grant, review and approval, contract signing, release approval and post-loan management, and use it as a key reference to make general evaluation and credit decision.
The bank strengthened financing risk control for enterprises involving "phasing out outdated production facilities". It urged to enhance management of financing related to the phasing out, timely organized bank-wide risk screening, and urged customers to complete the phasing out. ICBC continued to enhance financing risk control in areas involving heavy metal emission through rigid control methods such as financing suspension system that helped to step up reduction and withdrawal of existing financing as well as strict control over new financing.
The bank redefined the Green Development Strategy. The growth rate of loans supporting the green economy was 3.99 percentage point higher than that of overall corporate loans.
True leader Front runner Follower Laggard
BankTrack has assessed ICBC on its implementation of the UN Guiding Principles on Business and Human Rights in June 2016. ICBC is assessed as a Laggard, with a total score of 0.5/12.


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Use our free step-by-step guide below or have a reliable service form a Corporation for you.
If you want to save time and focus your energy on starting a company, here are two great online incorporation services:IncFile (for $49) Rocket Lawyer (for $99)
The very first thing to do is ensure that the name you'd like isn't already taken, doesn't contain restricted words, or is too similar to another name under the eyes of state law. Before doing anything else, use the Alabama Business Entity Search system to check, and if there are potential trademark issues conduct another quick search using the TESS System through the U.S. Patent Office.
Keep in mind your brand name should contain the words 'Corporation,' 'Company,' 'Incorporated,' or 'Limited." Abbreviations for these words are also acceptable. If needed, you can file a Domestic Entity Name Reservation form.
Filing Fee: $10 fee with expedited options
Every Alabama corporation, or business entity really, must elect a certified Registered Agent to represent their company. They're responsible for receiving and helping to handle legal paperwork like all state filings & important business forms, legal notices, etc.
Basic requirements are that this agent be an Alabama resident/company with an Alabama street address. That said, you can hire an outside professional and expect to pay up to $160/year, or get a certified/qualified agent for free when you incorporate with IncFile or CorpNet.
Most states have you file your corporation with the Secretary of State, but in Alabama you file a Certificate of Formation with the Judge Probate Court Office through mail in the county where your corporation's going to do business. Make sure you attach a copy of the Business Name Reservation form when you file if you need to file one.
When your Certificate of Formation has been filed with the Probate Court, they'll provide a stamped copy of your form and forward the original documents to the Secretary of State along with your $100 filing fee.
While not mandatory, this is very common practice among corporations because of the nature of the business entity. It's in this book you should keep all critical pieces of red tape to ensure you're well-organized, to protect your status legally, and come across professionally.
As the name of the book implies, it's a record holder of your Alabama Corporation registration documents, business licenses and permits, minutes of meetings, and other important documents. We're huge fans of Corporate Kits which include gorgeous records books/binders you can brand for as little as $99.
Appoint at least 3 directors who among other things will oversee the Alabama corporation until the first shareholder meeting where new directors will be voted in. Prepare an "Incorporator Statement" with complete names and addresses of each director and keep it in your records book. Once elected, an initial meeting will:
Ideally, during your first shareholder meeting you spent some time going over where to bank and set up your corporation's financial foundation. With so many different banks (local, state, national) with tons of different fee structures, incentives, kickbacks, and other business services this shouldn't be taken lightly.
The other point is to make sure corporate accounts aren't connected or mixed with any accounts of any other kinds. It should be a dedicated and separate account for easy financial tracking and tax purposes synced with your accounting software.
How many employees will your corp have? Where's it located and what kind of business are you in? Do you work with the public or hazardous materials? To ensure that you will not face penalties, fines, and fees when operating a business, you need to completely fulfill your business taxes and licenses obligations to the state. To get started, see the resources below:
Consider bookmarking the Birmingham district office of the Small Business Administration, and the Dept. of Revenue's Business Licensing section because they're going to be go-to sources. Also, consider investing in professional Business License Research packages can quickly get needed documents for you.
If you'd like help forming a Corporation, here are two great options:
IncFile ($49 + state fees) is ideal if you're on a budget but refuse to sacrifice quality. However if you'd like access to an attorney past incorporation Rocket Lawyer ($99 + state fees) is the best option.Visit IncFile Or Visit Rocket Lawyer
Note that this article on how to form a corporation in Alabama isn't a legal document or legal advice. It's for informational purposes and the information above is subject to change. For specific legal questions regarding how to form a corporation in Alabama or business in general, please consult with a lawyer or other accredited professional.


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Connecting Families to Senior Living
Talk to a local advisor for FREE
Talk to a local Advisor about this community
Contact this community at:
(866) 333-2174
140 Melbourne Rd
Caretypes Provided: Assisted Living, Alzheimer's Memory Care
Pricing is available for the following rooms:
*Prices quoted are monthly rental charges and are provided by the communities themselves. Actual prices may differ due to one-time fees, timing and care services required. Speak with your Senior Living Advisor to learn more.
Our apologies. Pricing is currently unavailable for this particular community.
Our neighborhood setting and spectacular views of the Berkshire Mountains create a beautiful residential atmosphere, one that easily allows us to feel like 'home.' The more than 100 residents at Melbourne are a diverse group in their 60's to 100's. Several couples live here, and an unusually high percentage of Melbourne residents are men! Daily social and cultural events engage residents on many levels. Residents benefit from our daily fitness classes, and state-of-the-art Keiser and NuStep exercise equipment. A hallmark of EPOCH Senior Living, our fitness program focuses on increasing strength, balance, range of motion, and vitality. Additional wellness education is promoted through Melbourne's registered nurse case manager, and informative lecture presentations with healthcare professionals. EPOCH at Melbourne offers gracious apartment-style assisted living, and additionally features Bridges; our program for individuals with Alzheimer's Disease and other memory concerns. Bridges offers special assisted living environment for individuals with Alzheimer's disease and memory impairment. The program works to bring out the best in residents, while offering security and comfort to their families. All Bridges staff members are certified through the National Alzheimer's Association's 'Care For People With Alzheimer's Disease' training program, promoting care giving within the context of relationship building between staff, the resident, and their family and friends. Our caring staff, many of whom have worked here since we opened in 1998, brings out the best in residents, offering personal care, security and peace of mind. EPOCH at Melbourne really is a community, in the truest sense of the word. Please come see, and feel, for yourself why we are 'The Residence of Choice for Seniors.'
Schedule an appointment with an Advisor
out of 4 reviews
Wingate Residences at Melbourne Place
"My mother called me today to tell me, that at age 94, she is in
love for the first time in her life. She loves the
retirement community, has friends now, has activities, and she has
blossomed. I was so sure she was headed for a dementia
unit and suddenly, she is this new person. I couldn't have done any
of this without your help and support every step of the way. You
have been amazingly helpful and supportive."
Caroline L., Found Assisted Living in Boston, MA
"My adviser checked in with us periodically just to let us know
she was there. I would not have been able to go through
this alone and knowing she was there eased the burden of making
this life-changing decision. Today my mom is happy and
Tricia M., Found Alzheimer's Memory Care in Quincy, MA
My mother is staying at EPOCH Assisted Living at Melbourne. They have been very attentive and very caring. They have shown that they are very genuinely interested in the well-being of my mother. more...
Call Now (866) 333-2174
Get the latest tips, news, and advice on aging and caregiving.
A Place for Mom is the largest assisted living referral service. We are paid by our participating communities, therefore our service is offered at no charge to families.
Copyright ©2017 A Place for Mom, Inc. All rights reserved.


text:
Moving to an Integrated Stress Environment Ecosystem
Increasingly, financial institutions are generating improved submission quality and synergies by taking a globally integrated approach to the scenario modeling of RRP, capital stress testing, and liquidity stress testing. While there are limits to the potential scope of this integration given inherent differences across these exercises, the need for a global strategic solution to scenario modeling is becoming clear as the regulatory requirements become more granular and as host supervisors demand local exercises for subsidiaries.
In this report, Moving to an Integrated Stress Environment Ecosystem, the authors lay out a Target State Vision of an integrated approach to stress exercises and a practical roadmap for financial institutions to achieve these efficiencies.
James Warrick Alexander, Principal in the Americas Finance & Risk and Public Policy Practices
Deepak Kollali, Partner in the Americas Finance & Risk and Public Policy Practices
Eric Czervionke, Partner in the Corporate & Institutional Banking Practice
Paul Cantwell, Partner in the Americas Finance & Risk and Public Policy Practices
Celent is a research and advisory firm dedicated to helping financial institutions formulate comprehensive business and technology strategies. Celent publishes reports identifying trends and best practices in financial services technology and conducts consulting engagements for financial institutions looking to use technology to enhance existing business processes or launch new business strategies. With a team of internationally based analysts, Celent is uniquely positioned to offer strategic advice and market insights on a global basis. Celent is a member of the Oliver Wyman Group, which is a wholly-owned operating unit of Marsh & McLennan Companies [NYSE: MMC].
Media Contacts
North America
Michele Pace
Tel: +1 212 345 1366
Europe (London)
Chris Williams
Tel: +44 (0)782 448 3336
Asia (Tokyo)
Yumi Nagaoka
Tel.: +81 3 3500 3023
Table of Contents
1. Bringing Together the Stress Environment Exercises
2. Target Vision for an Integrated Stress Environment Ecosystem
3. The Practical Feasibility of Achieving the Target State
4. The Roadmap Ahead


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Code of Conduct for Athletes
of Philosophy
It is the intent of the Schoharie
Central School District to provide a
well-rounded, educational experience for its students. Interscholastic
athletics are a major part of the educational process. The combination
of academics and athletics enhances the quality of our students' daily
lives. However, the District's primary focus will always remain
Opening Statement
Students who are representing the
Schoharie Central School District
in interscholastic athletics should realize that they have an obligation
themselves, their teammates, and the school community to strive for
It is hoped that participation in our
interscholastic athletic program will enable these students to value
their learning experience, to develop self-control, to exercise mature
behavior and judgment, and establish a sense of pride in themselves,
their school, and their community.
Participation in our interscholastic
athletic program demands a commitment that definitely involves sacrifice
and dedication. Respect for one's health and physical development must
be an integral part of daily living.
The following rules are set forth to
help all participants properly condition
themselves mentally and physically in order to perform well in the
classroom and in athletics.
The Code of Conduct is in effect during
the athletic season in which the athlete is participating. The season is
defined as beginning with the first day of practice set by Section 2 and
the Western Athletic Conference and ending with the last contest in that
sport which includes post-season play.
An athlete and his/her parent/guardian
will sign the Code of Conduct at the beginning of the athlete's sports
season. If the athlete signed an athletic code in a previous season, but
failed to sign one for the current season, the code is still in effect.
An administrative oversight does not void the code. It is assumed that
the athlete understands and agrees to the terms of the code even though
the athlete did not sign a new one.
Athletic / Academic Eligibility
A) Player/Parent Night
All athletes are required to attend a mandatory Player/Parent meeting
that is held prior to the team's fi rst contest. Any athlete, who along
with their parent/guardian that is not in attendance, will be ineligible
for games and scrimmages. Sign-ins will be taken at the meeting.
Emergency meetings will be at the discretion of the Athletic Director.
B) Academic Achievement is the primary
focus of the Schoharie Central School District. Athletes are not only
expected to maintain, but will be encouraged to improve academic
performance during any sports season.
After the first five weeks of school,
the high school office issues the Academic Probation/Ineligibility list.
All in-season athletes who have two or
more failures and/or incompletes will be
athletically ineligible (prohibited from competing in games or
scrimmages). The ineligibility will begin one week starting on the first
Monday after the infraction.
Athletes who are ineligible will be
required to take a copy of their schedule to each of their teachers for
them to sign indicating their current status (passing or failing). This
sheet must be turned in to the ISS room by the end of the day each
Friday. This process is to be followed for the entire five weeks.
The Teacher, Coach, and Athletic
Director must confirm that the student-athlete has only one failing
grade before the athlete can return to competition. Athletes are
permitted to practice with their teams during this time but cannot
compete in contests.
An athlete with one failure is eligible
to participate in games and scrimmages, however, if the athlete fails to
complete their requirements, then they will become ineligible. Probation
requirements are the same as ineligible requirements. Additional
administrative disciplinary action may cause ineligibility.
Student athletes must be in school by
8:20 a.m. unless they have an official written excuse.
Use of Alcohol / Drugs / Tobacco Products
Respect for one's health and physical
development must be an integral part of daily living. Health research
clearly indicates the harmful effects of using alcohol, drugs, and
tobacco products.
It is the intention of the Schoharie
Central School District to offer athletes the opportunity to make
responsible, well-informed choices based on honesty and integrity as
well as to offer help to those students who exhibit a substance abuse
A) First Offense –
Suspension from competing in games for 25% of the scheduled
games. (The schedule given by the Western Athletic Conference and the
athletic office will be used to determine the number of games)
Suspension will take effect with the first game after the offense and
will include non-league games. Athletes are expected to practice with
their team during this time but cannot compete
in contests.
Any identifiable cyber image, photo, or
video that implicates a student athlete to have been in possession or
presence of drugs/ alcohol/tobacco, or portrays actual use, or
out-of-character behavior or crime, shall be confirmation of a violation
of the Code.
If no determination can be established
for time frame or location of such action, the Code will still be
enforced. A coach's decision to enforce stricter rules will be honored.
Athletes who are honest and admit their
involvement when first questioned will have a reduction in the penalty
to 12.5% (minimum penalty for first offense is one game). For
determining number of games, if 25% or 12.5% does not figure out to a
whole number, i.e., 1, 2, 4, etc., then the number of games will be
determined by the following method:
Up to and including .5 will be rounded
down (i.e., 1.4 will be a one game suspension); .51 and up will be
rounded up (i.e., 1.6 will be a two game suspension).
Furthermore, the Athletic Director
reserves the right to push to the next tier if athlete is dishonest or
maintains a negative attitude. If an athlete is a captain, they will
lose that position for the remainder of the season.
A meeting with the school's counselor
and an outside substance abuse counselor,
agreed upon by parent and school, is mandatory.
A meeting between the Principal,
Athletic Director, parent and athlete before returning to competition is
mandatory. In addition, student will be required to re-sign the code in
the presence of the team.
B) Second Offense -
Suspension from competition in games for 50% of scheduled
games. (See first offense for determining number of games and what
constitutes a
game) Athletes are expected to practice with the team during this time
but cannot
compete in games.
Athletes who are honest and admit their
involvement when first questioned will have a reduction in the penalty
to 25%. Also, athletes and/or parents volunteering information about
themselves or a family member will have the penalty reduced to 25%.
If an athlete is a captain, they will
lose that position for the remainder of the
season. Athletes will not be eligible for any school sport award at the
end of the
season. This includes school letter, WAC All-Star recognition in that
sport, and any
Booster Club sponsored awards.
A formal substance abuse evaluation by
the school's counselor or equivalent,
agreed to by parent and school, and a follow-up to evaluation as
recommended by the initial evaluation is mandatory.
Mandatory meeting between the
Principal, Athletic Director, parent, and athlete
before returning is mandatory. In addition, student will be required to
re-sign the
code in the presence of the team.
C) Third Offense –
Suspension from participation (practice and competition) for 12
months from the date of the offense.
For reinstatement to occur, the athlete
must become a participant in a chemical
dependency program, treatment program, and/or education/instruction
program –
whichever is deemed necessary.
A mandatory meeting between the
Principal, Athletic Director, parent, substance
abuse counselor, and coach before returning to participation is
mandatory. In
addition, student will be required to re-sign the code in the presence
of the team.
These offenses are for the
athlete's entire athletic career starting with
their first involvement at the high school level.
If a penalty cannot be fulfilled
because of an occurrence late in the season
or due to the fact that the athlete does not complete the season, it
will be carried over the next sports season in which the athlete
The number of games suspended in the
new sports season will be based upon the percent served in the original
offense season subtracted from the total percent to be served.
When questioning athletes regarding
code violations, whenever possible, a third party will be present. This
third party will be an administrator or teaching staff member.
Athletes are expected to conduct
themselves at all times in a manner that refl ects
positively on their team, school and themselves. Violations under this
section will be reviewed, and a discipline committee consisting of the
Superintendent of Schools, Building Principal, Athletic Director, and
one coach will make determinations.
If a student has in-school suspension
(ISS), then they are ineligible for that day. They may attend a practice
or contest if the coach requests it. If a student is out-of-school
suspended (OSS), then they are ineligible and may not attend a practice
or contest during the term of the suspension.
Team Rules
Team rules reviewed and approved by the
Athletic Director will be given to the athletes by their coaches.
Equipment and Uniforms
Equipment and uniforms issued to each
athlete must be properly cared for and not
abused. It is the responsibility of the athlete to return all equipment
and uniforms in good condition to their coach at the end of the season.
The athlete must pay for all items not returned in good condition. The
athlete will be on the Ineligibility List until this matter is resolved.
Hazing / Initiation
Hazing or initiation by athletes is
prohibited by the Schoharie Central School District. Violation of this
section will result in suspension and/or removal from the team.
Medical Exam
The school's physician will administer
athletic physical exams yearly. The physical exam is required before an
athlete will be allowed to participate. The school physician has the
absolute authority to determine the capability of the athlete to
participate in a particular sport.
The athlete must fill out the Health
History form and return it to the school nurse before participating in
any practices.
Schoharie Central School Concussion
A concussion is defined as a type of
traumatic brain injury caused by a bump, blow, or jolt to the head that
can change the way your brain normally works. Concussions can also occur
from a blow to the body that causes the head to move
rapidly back and forth.
If a concussion is suspected proceed
with the following steps:
Remove child from event.
Refer to Concussion Danger Signs
information sheet for immediate medical evaluation.
Evaluate child using Concussion
Checklist provided.
Inform parent or guardian.
Refer for medical evaluation if
indicated in accordance with the Concussion Checklist.
Continue to monitor child until
child is under parental or medical supervision.
Notify school nurse – File incident
Return to Play Guidelines
After a concussion has been diagnosed,
proceed with the following steps:
Documented medical clearance is
required by a medical doctor before the child may return to physical
and/or sports.
The child should not return to
physical education and/or sports until the child is totally
asymptomatic (including after exercise).
The child must complete the Return
to Play Protocol below before he/she may return to physical
education or sports and the protocol must be completed with the
coach and nurse monitoring progress.
Graduated Return to Play
Rehabilitation stage: 1. No activity
Functional exercise at this stage:
Complete physical and cognitive rest.
Objective of stage: Recovery
Rehabilitation stage: 2. Light
aerobic exercise
Functional exercise at this stage:
Walking or stationary cycling keeping intensity <70 percent MPHR; no
resistance training.
Objective of stage: Increase HR
Rehabilitation stage: 3.
Sport-specific exercise
Functional exercise at this stage:
Running drills in soccer, volleyball, basketball, and cross country; no
head impact activities.
Objective of stage: Add movement
Rehabilitation stage: 4.
Non-contract training drills
Functional exercise at this stage:
Progression to more complex training drills, eg; passing drills in
soccer and basketball; may start progressive resistance training.
Objective of stage: Exercise,
coordination, and cognitive load
Rehabilitation stage: 5. Full contact
Functional exercise at this stage:
Following medical clearance, participate in normal training activities.
Objective of stage: Restore confidence
and assess functional skills by coaching staff
Rehabilitation stage: 6. Return
to play
Functional exercise at this stage:
Normal game play
Six day return to play protocol.
Each day the athlete makes a stepwise increase in functional activity,
is evaluated for symptoms, and is allowed to progress to the next stage
each successive day if asymptomatic.
Insurance and Accidents
Interscholastic athletics involve the
risk of injury to the participants. In case of an injury, coaches and
qualified school personnel are authorized to provide first-aid and
arrange for emergency treatment they deem necessary. Schoharie Central
School District carries a secondary insurance policy for injuries.
The parent's insurance will be billed
first. The school's insurance may not completely pay all bills. The
parent will be responsible for bills not paid by insurance. It is the
responsibility of the athletes to report all injuries to their
coach immediately. The coach will then complete appropriate accident
reports and file the report with the school nurse.
Transportation of Athletes to Away Contest
The Athletic Director will arrange all
transportation. All athletes are expected to ride to and from away games
with the teams. A coach will accompany the team on all trips and have a
complete travel list.
An athlete with a written request
signed by their parent may travel from an away contest with a parent.
The School Principal or Athletic Director must sign this request before
given to the coach.
Athletes are expected to follow all bus
rules. Any violations will result in disciplinary actions. Parents are
expected to pick up their children after practice, scrimmage
or games in a timely manner.
In order to promote the fundamentals of
good sportsmanship, student-athletes,
coaches, and parents will respect each other and the opposing school at
all times, including off campus and all internet communications.
Spectators and/or parents will not interfere with play during practices,
scrimmages, or games. Violations of good sportsmanship will result in
the issuing of a yellow(warning) card or a red (vacate) card.
Team Parent/Guardian
Each team is required to have at least
one parent/guardian who will serve as a liaison to the All-Sports
Booster Club.
The penalties set forth in this Athletic Code of
Conduct are in addition to any penalties that may be imposed by the
School's Code of Conduct.
Thank you very much for
your support of Schoharie Central Athletics
Updated July 2012; posted to web
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This page is maintained by Schoharie Central School
District and the Capital Region BOCES Communications Service according to the
web publishing guidelines of Schoharie Central School District. Copyright ©
2011. All rights reserved. PDF documents on this website can be viewed using
Adobe Acrobat Reader, which can be
downloaded from Adobe for free. Paper
copies of downloadable documents may be requested by calling 518 295-6679.


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