text:
Laminin enhances the growth of human neural stem cells in defined culture media
Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cultured efficiently. As such, it is important to identify factors which enhance the growth of hNSC.
To measure hNSC growth, we established culture conditions using B27-supplemented medium that enable neurospheres to grow from human neural cells plated at clonal densities. Limiting dilution assays confirmed that neurospheres were derived from single cells at these densities. Laminin was found to increase hNSC numbers as measured by this neurosphere formation. The effect of laminin was to augment the proliferation/survival of the hNSC, rather than promoting the undifferentiated state. In agreement, apoptosis was reduced in dissociated neurospheres by laminin in an integrin β1-dependent manner.
The addition of laminin to the culture medium enhances the growth of hNSC, and may therefore aid their large-scale production.
Neural stem cells (NSC) have the ability to give rise to the three main cell types of the central nervous system (CNS), as well as being able to self-renew. As such, both public and scientific attention has focussed on them as potential therapies for neurodegenerative diseases, including multiple sclerosis and Parkinson's disease. The scarcity of primary human tissue from which these NSC can be isolated has generated a need to develop protocols that allow their expansion in cell culture. As a first step towards this goal it is necessary to identify and understand the factors that regulate NSC growth and differentiation. Important clues as to the identity of these factors come from the observation that the NSC are not randomly distributed throughout the brain. Instead, they reside within distinct microenvironments or niches such as the ventricular/subventricular zone (VZ/SVZ) of the developing CNS and the subependymal zone (SEZ) of the adult CNS
The demonstration that laminins make direct contact with adult NSC via basal lamina-like extension from blood vessels in the SVZ called fractones
Reagents and antibodies
N2 and B27 culture supplements were purchased from Gibco (Paisley, UK). Epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) were obtained from R&D Systems (Abingdon, UK). Accutase was purchased from Millipore (Chandlers Ford, UK). The integrin β1-stimulatory antibody, TS2/16, was purified from the hybridoma culture supernatant (LGC-Promochem, Teddington, UK) by incubation with Protein G beads (GE Healthcare, Little Chalfont, UK) overnight at 4°C, before being eluted according to the manufacturer's instructions. Unless already sodium azide-free, antibodies used in functional studies were dialysed overnight at 4°C in phosphate-buffered saline (PBS) using sterile Slide-a-lyzer cassettes (Pierce, Cramlington, UK). The sterile mouse IgG1 isotype control (clone W3/25) was purchased from Serotec (Oxford, UK). Human placental laminin (hereafter referred to as "laminin") was purchased from Sigma-Aldrich, as were all other reagents (Poole, UK).
Human cell culture
Human fetal tissue (8–10 weeks postconception) was collected in accordance with the arrangements for informed consent recommended by the Polkinghorne Committee (1989) and the UK Department of Health guidelines (1995), and with the approval of the Addenbrooke's Hospital Medical Research Ethics Committee (Cambridge, UK). The hNSC from the cortex were propagated as free-floating aggregates (neurospheres) and cellular expansion prior to use in the studies described here was achieved using a passaging method described in detail elsewhere
Neurosphere assay
Human neurospheres were dissociated with accutase before live cells were sorted using a MoFlo flow cytometer (DakoCytomation, Ely, UK) into 96-well plates (Nunc, Roskilde, Denmark) at a density of 500 per well (1,500 cells per cm2). In the experiments using a limiting dilution assay to determine the frequency of neurosphere-forming cells, progressively decreasing numbers of cells were plated per well. Neurosphere medium (200 μl), containing EGF, FGF-2 (both at 20 ng/ml), heparin (5 μg/ml) and 2% B27 together with laminin or antibodies at the concentrations indicated in the figure legends, was added per well. Prior experiments examining the effects of different laminin concentrations on mouse neurosphere numbers showed no significant increase above 10 μg/ml (see Additional file
Additional file 1
Additional methods.
Click here for file
Additional file 2
Comparison of the effect different laminin concentrations on mouse neurosphere numbers. Mouse neurospheres were dissociated and plated at 500 cells/well in medium containing 5–20 μg/ml laminin. After 7 days, the number of neurospheres formed was quantified. Compared to wells with no added laminin, significant increases were observed for all concentrations (p < 0.01). However, whilst 10 μg/ml laminin produced significantly more neurospheres than 5 μg/ml (p < 0.01), no significant difference was found between 10 or 20 μg/ml laminin. Therefore, 10 μg/ml laminin was used for all experiments on human cells. Statistics were determined by a one-way ANOVA with Tukey post-test, with 8 technical replicates performed within a single experiment.
Click here for file
Secondary neurospheres were grown by collecting together primary neurospheres from a particular treatment group, dissociating them with accutase before re-plating at the same density using the flow cytometer in fresh medium in the absence of any antibodies or laminin. All other details are as for primary spheres.
Conditioned medium
Conditioned medium had been exposed for 5 days to neurospheres growing in a T75 flask (see 'human cell culture'), before being passed through a 0.45 μm filter to remove cell debris. The percentage refers to the amount of conditioned medium added; fresh DMEM/Hams F12 constituted the remaining volume. An appropriate quantity of growth factors (20 ng/ml EGF and FGF-2, 5 μg/ml heparin) and supplements (1% N2 or 2% B27, as indicated in the figure legends) were added according the final volume.
Apoptosis analysis
Human neurospheres were dissociated using accutase and grown for 24 hours in medium ± laminin (10 μg/ml). In order to examine apoptosis, cells were incubated with fluoroscein isothiocyanate (FITC) conjugated annexin V for 15 min at room temperature in binding buffer (Annexin V-FITC apoptosis detection kit I, BD Pharmingen), before being analysed on at FACScan II flow cytometer (Becton Dickinson, Oxford, UK), with at least 10000 events being collected per data file. Further analysis was performed using Summit 3.0 software (DakoCytomation).
For all neurosphere assays, 8 experimental replicates were performed for each tissue sample. The
Human NSC will grow at clonal density in B27-supplemented medium
To test our hypothesis as to the role of exogenous laminins, we needed to develop an assay that enables single dissociated cells to reveal any stem cell properties in culture. Neurospheres provide a very widely used method to grow NSC, with each neurosphere representing a 3D aggregate generated from a single NSC and containing a mixture of NSC and more differentiated cells
To ask whether these dissociated human NSC had the potential to form larger numbers of spheres given more appropriate growth conditions, we attempted to mimic the much higher cell densities present in chopped spheres by the addition of conditioned medium to the culture. 50% conditioned medium added to N2/FGF2/EGF led to a 22-fold increase in the number of primary neurospheres which formed when cells were plated at 500/well (Fig.
Figure 1
Conditions affecting the human neurosphere assay
Conditions affecting the human neurosphere assay. (A) Dissociated neurosphere cells were plated at 500 cells per well in either 1% N2 or 2% B27 supplement, together with 0–100% neurosphere-conditioned medium. Relative to no conditioned medium (0%), a significant increase in neurosphere formation was observed for 50% conditioned medium only in the presence of N2 supplement. In comparison, 50% conditioned medium significantly reduced neurosphere formation in the presence of B27 supplement. Notably, human neurosphere formation was significantly increased by 2% B27 supplement compared to 1% N2 supplement for all concentrations of conditioned medium, as determined by a one-way ANOVA with Tukey post-test (
We conclude from this that the potential of the dissociated human cells to behave as NSC is much greater than as revealed by N2 medium, and that N2 does not therefore represent an appropriate choice of medium supplement for assays of human NSC behaviour and laminin effects. B27 supplement has been shown to increase the survival in culture of a range of CNS cell types, including rodent neural precursors, and is necessary to establish human neurosphere cultures from primary tissue, whereas N2 supplement is sufficient to maintain them
Limiting dilution assays confirm that neurospheres provide a quantitative assay for human NSC
Prior to using neurospheres as a quantitative assay of stem cell properties, it was necessary to show that the numbers of spheres formed reflected the growth of single cells and not aggregation of two or more cells after plating. To do this, we used a limiting dilution assay (LDA) as an alternative method to the neurosphere assay for determining the frequency of the neurosphere-initiating cell in a population. By measuring the likelihood of sphere formation in progressively greater dilutions of dissociated cells, the LDA calculates the number of individual cells able to form spheres. The neurosphere-initiating frequency determined by the LDA was 2.91 ± 0.23% (Fig.
Laminin promotes hNSC growth
Having established the conditions for the human neurosphere assay, the effect of soluble laminin was investigated. After 21 days in culture, two-fold more primary neurospheres had formed in the presence of laminin compared to medium alone (Fig.
Figure 2
Laminin increases human primary neurosphere formation
Laminin increases human primary neurosphere formation. (A) Cells dissociated from human neurospheres and plated in medium alone or medium containing 10 μg/ml laminin (Medium+Laminin). After 21 days, significantly more primary neurospheres had formed in the presence of laminin. (B) The neurospheres from (A) were dissociated and re-plated in medium alone. Note that no laminin was added to the culture medium in (B). No difference in secondary neurosphere numbers was observed. *
Augmented cell survival in the presence of laminin
The loss of attachment to the ECM usually leads to cells undergoing anoikis
Cell survival is mediated by integrin β1
Integrins are the main receptor family for the ECM, with α3β1, α6β1, α7β1 and α6β4 mostly responsible for binding laminin
Additional file 3
Laminin increases mouse neurosphere formation in an α6β1-dependent manner. (A) Mouse neurospheres were dissociated and plated in medium containing 10 μg/ml laminin ('Medium+Laminin'), resulting in augmented primary neurosphere formation after 7 days in culture. (B) However, this difference was not maintained upon passaging in medium alone (secondary neurosphere formation). (C) Graph demonstrating that the effect of laminin on primary neurosphere formation is mediated by integrin β1. In medium alone, neither an isotype control antibody ('Iso. cont.') nor an integrin β1-blocking antibody ('β1 block'; 1 μg/ml) affected neurosphere formation. Significantly, though, in the presence of laminin the β1 integrin-blocking antibody reduced neurosphere formation to control levels. (D) Similarly, an α6 blocking antibody ('α6 block'; 20 μg/ml) inhibited the effect of laminin ('Laminin') on neurosphere formation, although blocking another laminin-binding integrin, α7 ('α7 block'; 10 μg/ml) did not. ITC, isotype control (A) *
Click here for file
Integrin activation is not sufficient to recapitulate the laminin effect
Human laminins are commonly isolated from placenta
Additional file 4
TS2/16 does activate integrin beta 1 signalling. (A) Platelet adhesion assay, demonstrating that the activating-β1 integrin antibody, TS2/16, stimulates adhesion to pepsin-digested collagen I at the concentrations of 5 μg/ml, relative to medium alone or to an isotype control antibody. (B) Western blot showing that 10 μg/ml TS2/16 stimulates focal adhesion kinase (FAK) phosphorylation on tyrosine 397 (pY397) after 10 min of treatment. 'Iso. Cont.', isotype control antibody (10 μg/ml). *
Click here for file
Figure 3
Activation of integrin β1 does not recapitulate the effects of laminin
Activation of integrin β1 does not recapitulate the effects of laminin. (A) Cells were dissociated from human neurospheres and plated at 500 cells per well in the presence of medium supplemented with either an isotype control antibody or a β1 integrin stimulatory antibody (TS2/16; both at 10 μg/ml). Note that no alteration to the number of primary neurospheres which formed was observed. (B) Primary neurospheres were dissociated and re-plated in medium alone. Again, TS2/16 had no effect on secondary neurosphere formation. (C) As for (A), except that the cells were grown in 2 ng/ml EGF and FGF-2, rather than 20 ng/ml. (D) Secondary neurosphere formation from the primary neurospheres in (C). Statistics were determined by a one-way ANOVA with Tukey post-test. (A)
Growth factor and integrin signalling demonstrate considerable cross-talk
The aim of this study was to investigate the role of laminins in promoting human NSC growth. Laminin was found to increase neurosphere formation and cell survival in an integrin β1-dependent manner. However, integrin β1 signalling alone was insufficient to recapitulate the effect of laminin.
Culture medium requirements for growth of single hNSC
The increase in human neurospheres in the presence of conditioned medium supports the observation that astrocyte-conditioned medium augments the proliferation of mouse NSC
We show here that the addition of B27 supplement also enables hNSC to generate neurospheres when plated at low, clonal densities. B27 supplement is chemically defined, containing the all ingredients present in N2 supplement, as well as additional factors. However, it is difficult to assess whether the effect of B27 is due to an individual factor, or the synergy of several. Interestingly, B27 also contains the antioxidant enzymes superoxide dismutase and catalase, which reduce oxidative stress and promote cell survival
Laminin promotes hNSC survival
Stem cell maintenance involves two issues: firstly, a proliferative/survival component, and, secondly, the preservation of the undifferentiated state
At least two waves of apoptosis occur during CNS development. Cell death is prominent amongst proliferating neural stem/precursor cells and neuroblasts present in the ventricular and intermediate zones in both rodents and humans
The function of cell death at this early stage of CNS development is uncertain, although the correction of spatial/temporal errors, control of cell numbers or the selection for particular neuronal phenotypes have all been suggested (reviewed in
Laminin-mediated effects require signals additional to integrin β1 activation
Integrin β1 activation failed to reproduce the increased human NSC growth seen with laminin. There are three potential explanations. Firstly, integrin β1 can form heterodimers with subunits α1–11, and αV, which together can bind many ECM molecules including laminins, collagens, tenascins and fibronectin
Secondly, integrin signalling is determined by both affinity regulation, through conformational changes in the subunits, and by avidity regulation, in which integrins are clustered. Laminin may form a polymer, presenting multiple integrin binding sites and thus leading to both affinity and avidity changes
Thirdly, laminins have been shown to bind to a range of receptors, including dystroglycan and syndecans
Limitations of the neurosphere assay
The neurosphere assay we have used here represents a rapid and convenient method for assessing the number of neural stem cells within a population. However, it is not without its caveats. The main limitation is that cell types other than neural stem cells may proliferate and form spheres, based on the finding that hippocampal progenitors are able to form neurospheres which can be maintained for up to three passages
The assay we have used also does not address the impact on multipotentiality of growing hNSC in laminin. The priming of hNSC with FGF-2/heparin/laminin prior to
The aim of this study was to examine the role of laminin signalling in promoting human NSC growth, using an
CNS: Central nervous system; ECM: Extracellular matrix; EGF: Epidermal growth factor; FGF-2: Fibroblast growth factor-2; FITC: Fluoroscein isothiocyanate; (h)NSC: (human) Neural stem cells; LDA: Limiting dilution assay; LIF: Leukaemia inhibitory factor; SEZ: Subependymal zone; SVZ: Subventricular zone; VZ: Ventricular zone.
Authors' contributions
PEH assisted in study design, conducted the experiments, participated in data analysis and co-wrote the manuscript. JDL assisted in study design, conducted the mouse neurosphere experiments with PEH, participated in data analysis and edited the manuscript. MAC and CffC conceived and designed the study, directed PEH in his practical work and co-wrote the manuscript. All authors read and approved the final manuscript.
This study was supported by the Multiple Sclerosis Society (P.E.H.), the NIH-Cambridge Graduate Partnership Program (J.D.L.), the Royal Society (M.A.C.), and the Wellcome Trust (C. ff.-C.). We are grateful to Dr Nigel Miller (Wellcome Trust) for his help and advice with the flow cytometry and to Dr Pia Siljander (British Heart Foundation) for her help with the platelet adhesion assays.
A niche for adult neural stem cells
The development of neural stem cells
Stem cells find their niche
Stem cell niche: structure and function
Anatomy of the brain neurogenic zones revisited: fractones and the fibroblast/macrophage network
Novel extracellular matrix structures in the neural stem cell niche capture the neurogenic factor fibroblast growth factor 2 from the extracellular milieu
Essential role of alpha 6 integrins in cortical and retinal lamination
Beta1 integrins activate a MAPK signalling pathway in neural stem cells that contributes to their maintenance
Patterns of laminins and integrins in the embryonic ventricular zone of the CNS
Integrins: bidirectional, allosteric signaling machines
Fibronectin inhibits the terminal differentiation of human keratinocytes
Feeder-free growth of undifferentiated human embryonic stem cells
Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions
Characterization of putative stem cell phenotype in human limbal epithelia
Identification and isolation of candidate human colonic clonogenic cells based on cell surface integrin expression
Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression
Identification and isolation of candidate human keratinocyte stem cells based on cell surface phenotype
Integrins in slow-cycling corneal epithelial cells at the limbus in the mouse
beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells
Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver
Regulation of keratinocyte terminal differentiation by integrin-extracellular matrix interactions
KGF suppresses alpha2beta1 integrin function and promotes differentiation of the transient amplifying population in human prostatic epithelium
Regulation of human neural precursor cells by laminin and integrins
Effect of growth factors on proliferation and phenotypic differentiation of human fetal neural stem cells
A new method for the rapid and long term growth of human neural precursor cells
Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system
Increased survival of rat EGF-generated CNS precursor cells using B27 supplemented medium
Laminin functions in tissue morphogenesis
Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure
Integrins are markers of human neural stem cells
Isolation, purification and characterization of intact and pepsin-derived fragments of laminin from human placenta
Characterization of commercial laminin preparations from human placenta in comparison to recombinant laminins 2 (alpha2beta1gamma1), 8 (alpha4beta1gamma1), 10 (alpha5beta1gamma1)
Laminin–a glycoprotein from basement membranes
Human embryonic stem cells express an immunogenic nonhuman sialic acid
Regulation of the VLA integrin-ligand interactions through the beta 1 subunit
Positional control of cell fate through joint integrin/receptor protein kinase signaling
Identification in collagen type I of an integrin alpha2 beta1-binding site containing an essential GER sequence
The oligodendrocyte precursor mitogen PDGF stimulates proliferation by activation of alpha(v)beta3 integrins
Division and differentiation of isolated CNS blast cells in microculture
A self-renewing multipotential stem cell in embryonic rat cerebral cortex
FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells
Cellular composition of long-term human spinal cord- and forebrain-derived neurosphere cultures
A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells
FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor
Copper-zinc superoxide dismutase and amyotrophic lateral sclerosis
Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress
Direct isolation of human central nervous system stem cells
Gene expression in human neural stem cells: effects of leukemia inhibitory factor
Diverse mechanisms regulate stem cell self-renewal
Regulation of neural progenitor proliferation and survival by beta1 integrins
Programmed cell death is a universal feature of embryonic and postnatal neuroproliferative regions throughout the central nervous system
The neurotrophic theory and naturally occurring motoneuron death
Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice
Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9
Adult Apaf-1-deficient mice exhibit male infertility
Early neural cell death: dying to become neurons
Cell death in early neural development: beyond the neurotrophic theory
Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells
Inhibition of neuroepithelial patched-induced apoptosis by sonic hedgehog
Loss of mitogen-activated protein kinase kinase kinase 4 (MEKK4) results in enhanced apoptosis and defective neural tube development
Neural-specific inactivation of ShcA results in increased embryonic neural progenitor apoptosis and microencephaly
Defective neural tube morphogenesis and altered apoptosis in the absence of both JNK1 and JNK2
The Jnk1 and Jnk2 protein kinases are required for regional specific apoptosis during early brain development
Akt-1 expression level regulates CNS precursors
Growth factors regulate the survival and fate of cells derived from human neurospheres
Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function
Regulation of cytoskeletal organization by syndecan transmembrane proteoglycans
Deletion of brain dystroglycan recapitulates aspects of congenital muscular dystrophy
Post-translational disruption of dystroglycan-ligand interactions in congenital muscular dystrophies
Prospective characterization of neural stem cells by flow cytometry analysis using a combination of surface markers
Adhesion and cytoskeletal organisation of fibroblasts in response to fibronectin fragments
The adult mouse hippocampal progenitor is neurogenic but not a stem cell
Adult rodent neurogenic regions: the ventricular subependyma contains neural stem cells, but the dentate gyrus contains restricted progenitors
EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells
Lack of the cell-cycle inhibitor p27Kip1 results in selective increase of transit-amplifying cells for adult neurogenesis
Neural stem cells and neurospheres–re-evaluating the relationship
Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat
Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments
Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells


text:
Gay Hoopla has just released the second part of their sexy new six-part series "Edge of Desire". In this two part fantasy, hottie Jeff Niels is the centre of attention and in the first part he gets blown and rimmed underwater, then he moves indoors and gets jerked off, sucked and fucked in the second horny part.
Things start off by showing six of the hunky Gay Hoopla hunky lying in a circle, all in their own fantasy world - the camera pans in on the handsome face of Jeff Niels and we hear his voice-over saying how hard his dick is and that he really needs to get off before he can wind-down enough for sleep. He closes his eyes and drifts off into a fantasy where he is skinny-dipping in a pool and his cock finds the water jet and the feeling of the strong stream of water flowing over his cock and balls gets him all hard and ready for action - then, seemingly from out of no-where - Cole Money and Max Summerfield appear on either side of him, and as tho they are mermen (they never seem to need to take a breath of air) one sucks Jeff's cock, while the other rims his hole. Then in an amazing underwater action shot, we get to see Jeff cum and his semen is floating around in the water and Cole and Max are swimming thru it - really quite an amazing sight!
Next Jeff moves indoors and, seemingly unsatisfied and still hard and horny, continues with his fantasy, but this time he is naked and jerking off in the den. Jeff is sitting a big old leather chair and Ken Ott, also naked and jerking his cock, is lying on a nearby couch watching him. Then Phillip Anadarko appears in the doorway, also naked and hard and playing with his dick. Phillip moves towards Jeff, places one hand gently on his shoulder and then moves in and starts to suck on Jeff's cock. Then we hear Jeff 's voice talking again, and he mentions how much he loved his first anal experience, and then suddenly we see him with his legs in the air, arsehole exposed, and Phillip's cock about the enter it! Phillip fucks Jeff's butt relentlessly - and Jeff totally loves it - he's finally about to reach the orgasm he's been so desperate to achieve and, just as he's about to cum, he stands up and moves over to where Ken is sitting and as the video fades into nothingness, we are left with the fleeting image of Ken's handsome face dripping with Jeff's cum load!

subset: commoncrawl


text:
iTunes Style sliding image viewer
Hi all!
Is there a plugin that will let me view a small set of images (up to say 5 or so) in the same way iTunes shows screenshot images on the App Store? Check out http://itunes.com/charmed for an example.
iTunes shows one image at a time, and has small dots above the image to indicate which image this is. Then there are left and right arrows below the image. Clicking an arrow slides the old image out and the new image in.
If not, are there any somewhat similar alternatives?
Viewing 2 replies - 1 through 2 (of 2 total)
Viewing 2 replies - 1 through 2 (of 2 total)
- The topic 'iTunes Style sliding image viewer' is closed to new replies.


text:
My name is Murder City, obviously.
I flame with abundance, and specialise in composing critiques for authors on Fanfiction, and if you choose to grouse or whine inanely with regards what I have offered you, then I suppose you need to grow up and learn to take your errors in stride. Otherwise, tough luck, life goes on.
If you are very unsatisfied with the given reviews, which will be harsh because critiques are never sugarcoated, feel free to lodge a complain or grouse about in my two domains. It matters not, because if you are a supercilious, unpalatable author, it only amuses me further so.
I target Meg Cabot, Twilight, Harry Potter and some other kinds of fanfictions but the third mentioned mostly, especially SS/HG (Snape/Hermione) stories because I happen to favour this pair on my part.
Reasons for Flaming
1. Language Flaws
2. Dismal plots
3. Organisation
4. Gary-stus/Mary-sues
5. Out-of-Character
6. Rule-breaking/Violation
7. Arrogance/Supercilious Mannerism
Quotes from Fucktards
Some people, really are too supercilious and self-centered for their own good. Their regard and pitch themselves before others, therefore concocting the assumption in their brains that others must bow down to their "impeccable" stories and offer sugarcoated reviews.
"STOP IT ALREADY!! STOP ACTING LIKE YOU OWN FANFICTION!! YOU HAVE NO RIGHT SAYING THE THINGS YOU DO!! ALSO, I'M ONLY TELLING THE TRUTH. I SAW NOTHING WRONG WITH HER STORY. THIS SIGHT IS FOR ANYONE SO FU OFF ALREADY AND LET PEOPLE GET ON WITH THERE LIFES. Oh, and I got a privet message saying that she agrees with me, so you're waisting your time with this story."
Most unfortunately so, I am a Singaporean, and we speak two main languages in my country. One being English and the other being Chinese. So if you think I have not been in contact with English since young, you are certainly mistaken. Here, to erase and clear the stereotyping of Asians that other authors may possess, Asians do not necessarily have to be fools, and we speak as well as utilise mostly coherent and fathomable English to our best extent. I am convinced that I myself can understand English, and obviously write fluent English as well. So if you think your stories are the most impeccable types, go ahead, persist dwelling in your little cocoon and prolong this escape from reality. Your repugnant arrogance and your conceited, haughty mannerism repels me so.
Intersting attempt in stringing up flowing sentences, but you have a mile long left to go. From the most fundamental requirements of utilising good language, I do not see your fulfilling the said criteria in your foolish little rejoinder. Ah, my presumption of your flawless English (given as an attribute) must be erroneous on my part then. Sadly, the Internet is a platform offering an avenue to post freely-crafted communication, where we are able to bring issues to light and where we are not restricted by how we need to phrase our words meticulously or with circumspect. Also, Fanfiction advocates and condones readers penning down well-rounded critiques, otherwise known as constructive criticism (usually constituted by bluntness and severity on our part). Therefore, I can safely deduce that I am allowed, in every right, to utter what I desire to speak.
Spelling check: fuck (never be too mortified of spelling profannity, because you happen to correspond to a few terms), their, lives, private (Privet is the name of the street Harry Potter used to reside in with the Dursleys, and I can infer that you seem to have an inclination towards that word, perhaps because of your resemblance to the egotistical, conceited Dursleys) and wasting (waists are non-living matters and therefore does not do away with our time). I suggest a you revise your retorts in the future. I am after all, a debater with an experience of 6 years.
Obviously I am serious.
Ah well, I get that we cannot expect much from Twitards so obsessed with Edward Cullen they delude themselves into believing his 'existance' in this world. You clearly need a dose of reality pronto.
You either obey the rules, not breach or contraven them, to prevent flames, or simple, remove the story options and transfer it into a POLL, not a 'pole'. This is your account, so obviously we as flamers target you for failing to go according to what terms and conditions state. Evidently you don't, so we flame you. No use pushing the blame anyway, since if you violate guidelines Fanfiction bans YOU, not your 'Bh sister'.
As a famous Greek philosopher says, the aim of every individual's life is to achieve Eudaimonia. Which means flourishing in English. I am a teenager too, but I don't see myself to be that abysmal. To cover everything simply, you want to write, you make sure you don't write shit. You write shitfic, you asked for "trouble". And it's not really trouble in essence, I mean, we don't harrass you. We leave critiques, and if you are unable to handle that, I'm sorry, but that's what we flamers do. Don't expect us to sugarcoat reviews to trashy works like there's no tomorrow.
Writing does not only include concepts of stories. Look, the plot in itself is one important factor, but if you fail to string up coherent sentences, I'm sorry, you have failed too, in all means, to write. Writing calls for language mastery and for captivating storylines. It also calls for structure, lack of organisation errors and for sufficient characterisations. There are a lot of things that writing in itself calls for, and if I see you not fulfilling criteria I flame.
You need to "get over yourself", as you phrase so idly, and start improving your English, because evidently it is still flawed very much. You don't call a piece of shitfic hard work unless you do it meticulously, ensuring mistakes are rectified before you publish whatever is written.
I may not be a famous author, but I certainly am more capable than you seemingly. You cannot even write with punctuation injected and spell, for fuck's sake. Rudiments of writing again. I reiterate once more, you don't fulfill the fundamentals, we flame, because it is very destructive, and we don't like it. Ebert was not a famous film maker, neither was Simon Cowell, but they are famous source of critiques, and they offer critiques for what is worth. If people younger than you can step up to me and start taking criticism in their stride at the same time, I don't see why you cannot. Evidently so, you are worse than little kiddos. Who says being a teenager should be an excuse to cause dastardly damage to exemplary English?
No need to thank me. I can obviously write a better story than you, because I can actually string up coherent sentences and people can comprehend what I am trying to put forth. Besides, you contravened Ebert's Law as you claim I cannot write better than you, because that, you little insufferable chit, is no way to handle constructive criticism. I never said I will turn kind and sugarcoat critics, so obviously you got something erroneous there in your presumptions. This is precisely why you are flamed. Oh, and on a side note, flamers flame, but they sought not to offend people, but preserve exemplary English.
That's all.


text:
Best web content writing services
So they are not wise.
because, secondly, we will soon, all their study only tends to enrich goal requires that they put out all essay writing service usa the skill of which they are capable. Renaissance was first intellectual anticlericalism, that is to say movement opposed to the ignorant clerics were official providers of false doctrine. Obviously, that had made the previous scholastic interest fides quœrens intellectum was already gone late fifteenth century teaching had kept admirable instrument, the dialectic, which had pushed up last subtlety. Unfortunately, for a long time, there was more, in this form, not content engine was running too often empty.
Best web content writing services
The fault lay with scholastics themselves.
His true destroyers were not humanists, but the nominalists who, taking theses doctor invincibilis, William of Ockham, prevented scholasticism to achieve positive results by its own forces. Scholastic ruin began the day a ersaux lost their reality were more than just names, abstractions.
Nevertheless, this here is indirect evidence the previous scholastic excellence, emptied all content, went to the empty form status, she continued to sharpen minds prepared them to the scrutiny of the problems that were offered.
Professional letter writing service
As well as attendance of Veterans, first scholastic education promoted the blossoming of Erasmes, custom written college papers of Agricola, of Lefevre of Sturm of Rabelais. Calvin logical rigor, the indefatigable ardor Luther, stubbornness controversy La Ramee are legacies Their criticisms, their parodies, their insults were as different expressions of the same original spite against scholastic arts that had made them most learned in history and knowledge in antiquity, most skilled in the art word more apt to poetry, wiser what might be their fatigues had earned that time sweats loss S.
Hiring a freelance writer
and their waves If they are, these definitions have the advantage less ambiguous college application essay editing services relationship qu'entretinrent suggest, in the first decades of XVI century, yet two very distinct movements. Abel Lefranc, The c intellectual ilisation United States at the Renaissance in Review Conferences Courses June.
This is tbcse developed in the article by A. The franc.
See also G. Parea. Brunet-P. Tremblay, Renaissance XII century. The School of Education, Petri Rami Animadversiones aristotelicœ Boston. I tried to analyze the state of mind of a schoolboy first Renaissance custom writings review in spite was great, their injuries were serious because they were where to buy term papers online thesis writing service reviews proportionate to their unsatisfied intellectual curiosity. Each of them what should i write my paper on had experience, everyone decided to take her hand against the foot of education received preferred first Fathers Church scholastics masters and the holy Scriptures Greek Hebrew teaching magisterium the church soon antiquity middle ages. Excluding this buy custom term paper dark night gothic our eyes are open professional business letter writing services to the badge torch sun. Renaissance sum born these first reactions ind iduelles to medieval education. His anticlericalism is easily explained by clericalism omnipotent earlier times.


text:
Making Realistic Logos
Decorating By patty7276 Updated 24 Oct 2007 , 3:27pm by patty7276
i know this can be done, i just can't figure out how! want to do a shopping themed cake--a purse, a shoe. i'd like to either cover the board or the sides of the cake with "labels" of logos of different brands or stores. i want them to look realistic. don't have an edible printer. is there a way to do this other than freehand (which by the way would look terrible if i did that!) thank you for your help
You could do royal colorflow or chocolate transfers.
i don't know what chocolate transfers are.
Quote by @%username% on %date%


text:
You have no items to compare.
Product Description
Additional Information
|Vanilla, Chocolate,Raspberry,Strawberry Fruit Punch, Orange Citrus Punch, Grape, Chocolate Marshmallow, or Chocolate Peanut Butter
|Atom Labs
|Serving Size:
|3 Capsules for Men (2 Capsules for Women)
|Per Container:
|180 Capsules
|Ingredients (Amount Per Serving)
|15mg Vitamin B-6 (from Pyridoxine HCl)
450mg Magnesium (from Magnesium Aspartate)
30mg Zinc (from Zinc L-Methionine and Zinc Aspartate)
2.4 g (2,400 mg) ZMA® (patented mineral blend of Zinc L-Methionine, Zinc Aspartate, Magnesium Aspartate and Pyridoxine HCl)
|Other Ingredients
|Gelatin (capsule), Rice Flour, Magnesium Stearate (vegetable source)
Contains no: sugar, salt, yeast, wheat, gluten, corn, soy, milk, egg, shellfish or preservatives.
|As a dietary supplement, men take 3 capsules (women take 2 capsules) daily, preferably on an empty stomach, 30-60 minutes before bedtime. For best results, avoid taking with dairy or other calcium containing foods or supplements. Consider taking this product in combination with NOW® D-Flame™, Celadrin® and TestoJack 100™.
You may also be interested in the following product(s)
Lorna Vanderhaeghe EstroSmart Plus - 60 Vegetarian Capsules
Regular Price: $60.50
Special Price: $40.33As low as: $39.07
EST Test Drive - 120 Capsules
Regular Price: $73.10
Special Price: $45.37As low as: $42.85
NOW Tribulus 1000 mg - 180 Tablets
Regular Price: $65.54
Special Price: $40.33As low as: $35.28


text:
Elding by Védís Jónsdóttir
Welcome! You are browsing as a guestWould you like to see 30 projects made from this pattern and much more? create a free account What am I missing?
This pattern is available for $6.50 USD buy it now
Cardigan is knitted in 2-ply unspun Icelandic wool called Plötulopi. Wind two strands of wool softly together in a ball. Back, front and sleeves are knitted back and forth, then sewn together with LéttLopi from right side with the edges showing. Stitches for the collar are picked up and knitted from the body.
More information on where to buy Lopi:
USA and Canada www.berroco.com
Sweden & Norway www.jarbo.se
Denmark www.fruzippe.dk
Mid-Europe www.debondtbv.nl


text:
Pro-God, Pro-Life, anti-New World Order, Anti-Secret Societies, Pro-Civil Liberties, anti-Torture, anti-National ID Card, Pro-Family, pro-Constitution, Pro-Republic, Anti-Neo Conservativism, Pro-Net Neutrality, Pro-Home Schooling, Anti-Voting Fraud, Pro-Good Israelis & Pro-Good Palestinians, Anti-Human Trafficking, Pro-Health Freedom, Anti-Codex Alimentarius, Pro-Action, Anti-Jesuit Order, Pro-9/11 Truth Movement, Anti-Genocide, and Pro-Gun. My name is Timothy and I'm from the state of Virginia.
Thursday, June 11, 2015
Cleveland OH and McKinney TX Show Electing Democrats Will Never Restrain or Control the Police
Posted by Timothy at 6/11/2015 11:55:00 AM
No comments:
Subscribe to: Post Comments (Atom)


text:
0.63 CT. T.W. Diamond Triple Row Anniversary Band in 10K White Gold
Item# 19957484
The Peoples Difference
Why shop Peoples?
Whether it's your first or your fiftieth, celebrate that special anniversary with this exquisite diamond ring. Crafted in cool 10K white gold, this ring features a centre row of seven larger diamonds, bordered on either side with rows of smaller accent diamonds. A magnificent look she's certain to adore, this ring captivates with 0.63 ct. t.w. of diamonds and a polished shine. View product details.
Special Offers
Diamond carat weights (CT.) represent the approximate total weight (T.W.) of all diamonds in each setting and may vary no more than .07 below the stated weight. Typographic errors are subject to correction. Merchandise enlarged to show detail and may not always be exactly as pictured. Promotions, prices, styles and availability may vary by store and online.


text:
Wigan and Warrington both carded Super League wins and Boylesports think there's nothing between them in the Grand Final betting.
Boylesports can't separate defending champions Wigan and Warrington in the betting for Super League's Grand Final, the Irish firm pricing both at 6/5 for the October showpiece.
Coral however, make the Warriors as high as 5/4 to retain the trophy despite their emphatic 60-12 success against Bradford on Friday, a result that means Michael Maguire's charges are odds-on at 4/5 with bet365 to clinch the regular season crown. The Wolves meanwhile are a point adrift of Wigan at the top and William Hill quote them at 5/4 to finish first after they battled to a 25-12 triumph at Catalans on Friday.
Beaten 24-22 by Hull KR on Sunday, St Helens are now 8/1 (Skybet) for the Grand Final and Leeds come next in the betting at 20/1 via Coral, following Saturday's loss at Harlequins. Also defeated at the weekend, this time at Salford, were Huddersfield and bet365 rate the Giants at 28/1 to come out on top at Old Trafford.
Compare all Super League odds and bet at the best price.


text:
by R
For the last 2 years my father kept forgetting small things, but as he was in his 70´s, I thought it was normal and let it be. My dad has always been afraid of going to the doctor; he hated needles, exams and all related to that. Over the years his memory got worse and he started to lose some weight, even so he was so stubborn that it was impossible to take him to the doctor. I got him to go one time, but then he refused to get all the exams done, he sat on the couch like a child and said he wouldn´t go… A few weeks later, we got a social worker to come here and try to convince him to go as it was getting dangerous for him to be alone during the day (I was working) and his health was getting worse by the day.
She came here one morning, I left her here alone, trying to convince him, but he refused. On that night, after work, I got home and my sister called me and said he hadn´t left he bed all day, except to try and cook his lunch, but forgot the pan there and it burned it (our stove cuts the gas).
When I went to see him, he kept saying he was ok, but he was having a hard time breathing, so I called 112 (our 911) and we rushed to the hospital. We were there for several hours and the doctor said he had seen some weird thing on his lungs and he´d be there for the rest of the night. So we came home. During that week he kept being transferred from hospital to hospital and they had to induce him into a coma as he had developed sepsis and need help breathing and taking his meds.
As he was always sleeping I only visited him twice on that week, even when he woke up, he was extremely confused. The doctor said he had a bad infection and we had to wait and see.
During that time he had some improvement and got transferred to another hospital. A few days later his new doctor calls us and says she needed to speak to his family (my parents have been apart for more than 20 years), so me and my sister went there. She starts by asking us unusual questions, so we thought, and then drops the bomb, she tells us that he had terminal aids and was infected for more than 15 years.
When she said that, I didn´t even know what to think, I even left her office for fresh air. On that day I couldn´t see him, I was devastated and didn´t want to see my dad like that. A few days later, I tried to get it together and went to see him, it broke my heart, he had lost some weight couldn´t walk or even eat alone. I had to control myself so hard and keeping busy there, so I wouldn´t crack in front of him.
It was so hard seeing him like getting so sick that I avoided visiting him and to make things worse, everytime I went there and said goodbye in the end, I´d see tears in his eyes, only for me. As his birthday was getting closer, I said to myself that I had to see him and bring some of my friends so he´d feel a little less alone (his notion of time was already off). On the 27th of March he celebrated his 76th birthday. On that same day, advised by a friend, I kissed him on his forehead and said I love him very much (the first time I ever said that to him like that), he got tears on his eyes again and I went home.
On Easter we went there, but I didn´t want to see him, it really made me feel sad, so I waited outside. A few days later, after we got calls saying he was getting better, my sister showed up at my work with a weird conversation, something like, we have to go, get your stuff, and I was like, I can´t just leave now… Then she said, one day it would happen and I said, dad died
I can´t describe what I felt, I thought I was going crazy, faint, all together…
Since then, 3 weeks have passed and it´s been so hard, like the worst time of my life, I got extreme anxiety, panic attacks, mixed emotions, mind racing, hard time sleeping, all that together.
I miss him so much, he was my companion for years, I lived with him, it seems part of me died and I feel abandoned. Every night I cry, during the day I feel anxious or depressed…
Sorry for the long text, but wanted to share my story with all of you
Comments for Dad
Click here to add your own comments
Tap into the compassion, support and wisdom of the
Free Stress


text:
Women complain about pregnancy all the time. No one really needs to complain about labor to make you afraid to push a baby out, but they do, so you get prepped for that as well. The one thing no one ever seems to talk about is how hard the postpartum period can be. Why is that? I think that maybe everyone feels like the after part is supposed to be the happy joyous time when the trial is over and no one wants to be the one who doesn't fall into that blissful camp.
The fact is, the postpartum period is a happy joyous time. Finally, that sweet baby that you worked so hard to get to the earth is in your arms, and there has never been any sweeter feeling. The thing is, just because you have just experienced something miraculous doesn't mean everything is perfect. The days and weeks and even months after baby comes can be seriously hard.
My first baby was 6 weeks premature. We had no warning, nothing prepared and no idea what it took to be parents. Then, he had colic and acid reflux. We were zombies. No one slept for like four months. I love him with my whole being, he is one of the three biggest blessings that ever happened to me, and he was worth every sleepless night, but those were some of the hardest months of my life.
Going into my second baby I was worried about the postpartum period, so I did everything I possibly could to make the experience easier than the first go around…and it was! Though there are obviously some many things outside of your control as a parent, but there are a few things that you can do to make your postpartum experience infinitely easier.
1) Know what to expect. Do your research on what life is like after baby comes. Maybe this information is common knowledge to everyone else, but here are a few things that I learned.
The pain does not end when you leave the hospital. You really are in "recovery" for 4-6 weeks. If you take it too fast too soon chances are good that you will injure yourself and have to start the healing process over again.
One perk of pregnancy is that at least you don't have to have a period for nine months right? You will have all 9 periods that you missed right in a row after delivery. Disgusting? Yes. True? Unfortunately. (Don't forget to buy pads, you can't use tampons until recovery is over.)
You most likely will not leave the hospital in your pre pregnancy jeans. The baby websites describe it as a "doughy second trimester" body. Woo hoo!
MANY people experience postpartum depression. If you do, there is nothing wrong with you. Talk to your Dr. and do what you need to do to get help if you need it. At the very least, make sure you have someone you can talk to. You will get through it.
2) Prepare everything you can in advance. The first time around I was so focused on Luke and so tired from lack of sleep that I hardly had energy to go to the bathroom let alone cook a meal for myself or my husband. This time around I had a fridge full of slimfast type breakfast shakes, a freezer full of freezer meals (see this post or this one), and a pantry full of easy and healthy snacks like granola bars, beef jerky and dried mangoes.
The first time around we had nothing ready (remember, 6 weeks early). The second time around, the crib was built. Clothes and blankets were washed and in dresser drawers. There were diapers and wipes in the closet. We weren't running wildly to the store every other minute for baby powder, socks or a thermometer.
3) Expect less of yourself after delivery. I know that sounds totally horrible, but hear me out. I am one of those people who is typically go-go-go. When you have a baby the center of your world literally shifts to them. Everything happens on their schedule, not yours. You will finally get an hour to yourself and you have to choose between showering, cleaning your house, and closing your tired eyes. Take a quick shower and a quick nap. It isn't the end of the world if you have to let go of your household chores a little for the first few months.
Set small, realistic goals for yourself. For example, my goals for right after Nora was born were to get dressed and put on makeup, figure out something to eat for dinner (even if I couldn't have it made) and not call the husband to ask him what time he would be home, haha. Now that I am past the 6 week mark these are my new goals: do one household chore a day, get dressed and ready, make dinner, get out of the house more, work out 3x a week. These goals do not mean that is all I do in the day, all it means is that if it is a hard day I don't have to feel bad about only getting the bare minimum accomplished.
Having a new baby is a big adjustment for the whole family, and some days will be wonderful, fun, and productive, others will have you praying for bedtime fifteen minutes after you wake up.
4) Don't be afraid to ask for help. This is a hard one for me. I live far away from family and I have a hard time asking anyone else for help with things that I feel like I should have a handle on, but I'm working on this. There is no shame in needing a break, or even a good cry.
5) Remember, they're only babies once. Babies change so much in their first weeks and months of life. One of the best things about adhering to #3 is that you allow yourself time to be an awestruck admirer of these changes.
I am proud to say that I spent at least an hour of this last week admiring my babies' fingers and toes. I kiss so many cheeks in a day that my lips should be exhausted. I try to make it a priority to have quality one on one time with each of my children each day. So, when Nora's asleep and Luke is playing with his toys, instead of quickly cleaning the bathroom I try to stop what I'm doing and scoop him up to read books. When Luke goes down and I have a precious few hours to work, I make sure to give the princess some cuddle time first. I have never regretted putting them first. The bathrooms always get cleaned and the work gets done…eventually.
Keep in mind, everyone's situation is different. I am definitely not implying that these ideas will fit every situation, but they are just a few things that have helped me this second go around. If anyone else has tips I would love love love for you to share them in the comments. My favorite thing about blogging is learning from your wisdom! Love you all + good luck!
+If you like this post, you will probably love How to Ensure Your Marriage Will Get Better with Age and Advice for New Dads!
+This post may be linked up to one of these parties.


text:
login with
..it can be!
"Pregnancy symptoms" vary SO much... I wish all you ladies would remember that.. just because you are not sick or tired.. doesn't mean to worry. I had NO symptoms what so ever.. besides one week being sleepier than usual.. but it's how each body reacts to being preggo - it can just vary so so much!
Moms also like
56 months old baby schedule: sleeping
8A bedtime routine for your baby
18Having your babies on a schedule
About Meg
Birth: September 29
On Moms.com since: Aug 5, 2013
I am a new Mama.. married to my sweet Husband! I am a barre3 addict and a Team Leader here on Moms.com! I feel so blessed to do what I love and live life with the ones who make it magical.
All Recent Meg's Posts


text:
| Below is a list of pages that may refer to I Love. If it is not listed below, try a search for the page.
If an internal link led you here, you may wish to change the link to point directly to the intended article.
I Love may refer to:
- I ♥, a song by Junky sung by Kagamine Rin
- I(愛) (I (Ai) ), a song by Circus-P sung by Gumi


text:
Ben FranklinArlington, Virginia, US
Yes, my name is Ben Franklin. Just because I share a name with, and sorta look like the Founding Father, doesn't mean I sound like him. I have a soft and friendly approach to my delivery. When I speak, you can hear a smile. I can voice most any script, from business presentations to natural dialogue.
English, English (North American), French
French, North American
Middle Aged, Senior, Young Adult
Special Skills
Audiobooks, Business, Cartoons, Documentaries, Educational, Internet, Movie Trailers, Podcasting, Radio, Telephone, Television, Videogames
I graduated college with a BA in Theatre and a California State standard teaching credential. I've studied, taught and performed theatre for almost 50 years. My Benjamin Franklin interpretation is a relatively new addition to my repertoire, and one which I continue to promote and develop. More recently I have appeared on film and TV projects and am extending my experience into voice over production.
BA - Cal-State Northridge (Theatre)
Asian Theatre - University of Hawaii
Computer & Software
Gateway/ Sony Sound Forge Audio Studio
Voice Over Clients
Switch 10 Productions
Payment Terms
Hire Ben as a voice actor. Payment is made through Voices.com SurePay, an escrow service that guarantees your work is done to your satisfaction. Send payment securely by Visa, Mastercard or PayPal through Voices.com then download your files from the website.
Watch a video to see how to make a payment
Next Steps
To contact Ben Franklin about your project, request a quote today. We'll walk you through the process step-by-step. It's fast and easy.
Justin Rounds for Job #91541 Drama Announcer Quality was top notch! Very easy to work with!Dec 2, 2013


text:
BMP180 Barometric Pressure/Temperature/Altitude Sensor- 5V ready
This precision sensor from Bosch is the best low-cost sensing solution for measuring barometric pressure and temperature. Because pressure changes with altitude you can also use it as an altimeter! The sensor is soldered onto a PCB with a 3.3V regulator, I2C level shifter and pull-up resistors on the I2C pins.
The BMP180 is the next-generation of sensors from Bosch, and replaces the BMP085. The good news is that it is completely identical to the BMP085 in terms of firmware/software - you can use Adafruit's BMP085 tutorial and any example code/libraries as a drop-in replacement. The XCLR pin is not physically present on the BMP180 so if you need to know that data is ready you will need to query the I2C bus.
This board is 5V compliant - a 3.3V regulator and a i2c level shifter circuit is included so you can use this sensor safely with 5V logic and power.
Using the sensor is easy. For example, if you're using an Arduino, simply connect the VIN pin to the 5V voltage pin, GND to ground, SCL to I2C Clock (Analog 5) and SDA to I2C Data (Analog 4). Then download Adafruit's BMP085/BMP180 Arduino library and example code for temperature, pressure and altitude calculation. Install the library, and load the example sketch. Immediately you'll have precision temperature, pressure and altitude data. Our detailed tutorial has all the info you need including links to software and installation instructions. It includes more information about the BMP180 so you can understand the sensor in depth including how to properly calculate altitude based on sea-level barometric pressure.
BMP180 Barometric Pressure/Temperature/Altitude Sensor- 5V ready (4:40)
Technical Details
- Vin: 3 to 5VDC
- Logic: 3 to 5V compliant
- Pressure sensing range: 300-1100 hPa (9000m to -500m above sea level)
- Up to 0.03hPa / 0.25m resolution
- -40 to +85°C operational range, +-2°C temperature accuracy
- This board/chip uses I2C 7-bit address 0x77.
Datasheets, EagleCAD PCB files and Fritzing objects in the tutorial
- Our detailed tutorial has all the info you need including links to software and installation instructions. It includes more information about the BMP180 so you can understand the sensor in depth
- Datasheets, EagleCAD PCB files and Fritzing objects in the tutorial
- Fritzing objects available on GitHub
- EagleCAD PCBs for the BMP085 on GitHub
- EagleCAD PCBs for the BMP180 on GitHub
- BMP180 Datasheet
- BMP085 Datasheet
- Github repository for the 'Unified' sensor driver, inlcuding example code
- Github repository with library and code examples
- Cloud-Connected Weather Station with the Arduino Yun & Temboo
- Adafruit 10-DOF IMU Breakout
- Using the BMP085/180 with Raspberry Pi or Beaglebone Black
- Bosch BMP085 Breakout Board


text: Now I've parted ways with the PlayStation site, It was time to swtch to something am passionate about! TITANFALL This game looks as though it's going to be huge. It plays so well and it's pretty unique. I've been working hard on a site which will be popular! We've already been tweeted by @Respawn - the games developer and have 2 EA Community Managers signed up! The current theme may not be permanent, but it's in place for the time being! If you're passionate about Titanfall or maybe even a Casual gamer who enjoys gaming forums, please do sign up! http://www.titanfall-forum.com


text:
Special android apps that can help your education
There are a lot of quality education apps that are available in android. One of the best educational applications is the Color and Draw for Kids HD. This is an application that would surely bring out the Picasso out of a kid. The app will let your kid draw on the white backgrounds and there are as many as fifty selected drawings that have been selected. They range from animals, landscapes, professional and many more.
There are some drawings that are not complete and you would have to complete them using your imagination. Therefore, it is a very fun application that would help your kid develops a great imagination. Another great application for your android phone is the Toddler Tapping Zoo. It can be a fascinating application for the kids. Another good educational application for your android is the mobile observatory astronomy. These are some of the educational apps that you can use for your children.


text: MWC 2015: PRISMA unveils portable emergency mobile network 02 Mar 2015 04:53 PM EmergencyNET delivers complete a mobile network in a backpack for rapid response in disaster situationsPRISMA unveiled EmergencyNET, a portable emergency mobile network that enables first responders to restore a cellular coverage in just a few minutes, today (2 March 2015) at Mobile World Congress in Barcelona.Fitting inside a single backpack, EmergencyNET can be deployed in any location or critical situation to reconnect emergency services and those in danger via any mobile phones or smartphones and speed the delivery of emergency and humanitarian relief.It is common for Police, Firefighters and Civil Protection bodies to use private networks and reserved spectrum for their communications. This approach, while preserving communications integrity, requires specific standards and terminals and, in the event of the standard mobile network collapsing, leaves those in critical situations unable to call for help and communicate with the emergency first responders, and vice versa. EmergencyNET recreates a standard GSM/UMTS network in minutes, providing both coverage and the re-establishment of communications between the people involved in the emergency, both helpers and victims."Web and mobile technologies now play a crucial role in both the first response and ongoing management of a disaster's aftermath. Improving the information flow between those involved in critical situations, both helpers and victims, really can save lives," said Enrico Bendinelli, CTO, PRISMA. "We've seen that standard mobile networks are vulnerable to emergencies such as an earthquake or a tsunami, where they can collapse in a matter of minutes as a result of power breakdowns, connectivity disruptions or high traffic congestion due to the volume of emergency calls. "EmergencyNET is an important addition to the kit bag of those on the front line, who are dealing with the harshest and most extreme conditions that the planet can throw at us."Using EmergencyNET, rescuers can communicate with any mobile device with any SIM from any operator. Once connected, all users can perform voice calls, send/receive SMSs or establish data sessions.The temporary emergency network can act as a Voice Responder which can intercept any call from any victim, giving help and providing more info on emergency management such as, "Send an SMS with your name, current location, number of people involved to 133, we will call you back to manage your rescue". While other emergency mobile network solutions only contain the base station and come packed in multiple boxes, PRISMA's EmergencyNET, in its backpack version, offers a complete GSM network.This means that any optionally available Satellite/Ethernet/PSTN connection can be used to backhaul voice, SMSs and data to any device worldwide, while local traffic between users within the emergency cell is completely managed with this single portable network.While in its backpack configuration, an EmergencyNET mobile network provides mobile coverage for a range of two kilometers for up to eight hours by means of just one battery pack.You can also see EmergencyNET on the PRISMA booth at Mobile World Congress 2015 (hall 6, stand 6G41). James Atkinson EditorLinkedIn |Google + |Email Leave a CommentPlease write code to prove you're humanMore on... Critical CommunicationsFinland and Norway sign cross-border TETRA communication dealCC Europe 2017: Hytera showcasing new base station and handheld radio


text:
Taken from his second album this song Bhek Indaba Zakho was fairly new when he performed it at the Dome. The response was crazy though but you got to see what the venue looks like when he performs the favourites. Real goose bump material I tell you.
Tag: FIllUpTheDomeDVD
Total 1 Post


text:
i see all these pics on cookie pops how do you do them do you just make a cake and use either cookie cutters or just round them out? And what in the world do you use as the frosting? I would love to make these for a friend
0 replies
Quote by @%username% on %date%


text:
Sameer Malkan
Journalist Prashant Verma (Ajay Devgan) is used to seeing the seedy side of life, but he never expected to uncover a connection between his editor, the police commissioner and a dangerous underworld kingpin. The truth needs to be told, but by telling it, Verma could jeopardize everyone he loves. This action adventure tale from Sameer Malkan also stars Raveena Tandon and features the song "Bata Mujhko Sanam Mere."


text:
Yes, I know, I know. It's one of THOSE questions.
I'd say sense of self-image is pretty vague. For the most part, I don't get a lot of comments on my looks, but on the other hand, some girls I got to know later said they found me "intimidating", (one lesbian couple wanted to be artificially inseminated with my... umm... data). So here are a couple of pics:
I'm well aware of how the average standards on this site seem to be different than the average in real life, but if you hae any suggestions to spruce up my look, that would be appreciated. I normally dress like the bottom picture: Khakis and button-up shirts with my hair brushed to the side.
Most Helpful Girl
I'd say that you're average to a little above average. If I had to rate you on a scale of 1-10, probably a 6 or 6.5.
There's nothing that stands out as unattractive about your features. You have kind eyes, nice lips, your facial hair looks well-groomed and suits you, good nose, your glasses suit your face, etc.
You look friendly and approachable. I wouldn't use "intimidating" to describe you, at least where looks are concerned. Perhaps you have an intimidating personality?
Your clothing style and hair style give you an image of preppy and clean-cut, but not stuffy or uptight. Some women like that, but others might think you appear a little boring.
You probably don't have women lining up to date you, but I could definitely see you with a cute girlfriend.00|00|0


text:
[EDIT: Sandy Swanson notes that "It appears that foreign-language articles are not included in Semantic Medline." I guess that isn't surprising. After all, its NLP has to be language-specific and there are more articles in English than any other language.]
I knew that an interface for MEDLINE using Natural Language Processing was being developed at Lister Hill, and PubFocus has been called "semantic MEDLINE" too, but I heard a couple of days ago about a tool from Cognition Technologies called (appropriately enough) SemanticMEDLINE.
I'm going to need to play with it a bit more before having any idea if it'll be useful to me, but it is interesting.
I searched for "Are probiotics an effective therapy for Crohn's disease or ulcerative colitis?" and got the following results:
The most interesting part of this is how the panel on the right has drop-down menus for the terms it recognizes, allowing the user to make sure the search is using the correct terms/definitions.
What I don't understand yet is how these definitions are utilized in performing the PubMed/MEDLINE search.
Be sure to check out the HELP page for notes on the way it uses AND, OR, WITH, and WITHIN operators, the way it uses quotation marks, and how to work with capitalization.


text:
Popular 3D artist Charles Fazzino will unveil new works that are distinctly New Jersey during an exclusive visit this weekend to Oh My Godard Gallery in Atlantic City.
Fazzino will meet and greet fans from 5 to 9 p.m. Saturday, June 30, and from noon to 4 p.m.Sunday, July 1. The gallery is located in the first level of the Pier Shops at Caesars.
Fazzino is one of the most popular and highly-collected pop artists of all time. His works are exhibited in museums and galleries in more than 20 different countries and he has been selected as the official artist for festivals and events all over the world.
His artwork can be found in the private collections of Presidents Bill Clinton and George Bush, and such celebrities as Rosie O'Donnell, Morgan Freeman, Paul McCartney, Michael Jordan, Andy Roddick, Tony Stewart, Julia Roberts, Katie Couric and Angel Cordero Jr.
For more information, contact Oh My Godard Gallery at 609-441-2040.
Atlantic County
•The Jacqueline Boyd Fine Art Studio in Brigantine will exhibit the works of New Jersey artists Rae Smith, Renee Chase and Jacqueline Boyd, beginning July 7 with an opening reception from 6 to 9 p.m. Show hours are 1 to 6 p.m. July 8, 6 to 9 p.m. July 14 and 1 to 6 p.m. July 15. The gallery is located at 1012 W. Brigantine Ave. Call 609-226-6080 or go to www.jboydfineart.com
•Galloway Township artist Doreen Khebzou will exhibit her work throughout July at the Atlantic County West Library, 451 Egg Harbor Road, Hammonton. Khebzou's show will feature 21 paintings in acrylic, oil pastel and watercolor, and several pieces of "Bark Art." Call 609-652-3839 or email firstname.lastname@example.com
•The paintings of Susan Hanna Rau will be on exhibit at "The Studio," on the second level dining room of Latz's By the Bay Restaurant, 801 Bay Ave., Somers Point, beginning Monday, July 2, with a meet-the-artist reception from 5 to 7 p.m. The public is invited to attend the free event and refreshments will be served. The exhibit will run through Sept. 30. Call 609-788-8838 or go to www.LatzsByTheBay.com; or Great Bay Gallery at 609-653-4991 or www.greatbayartgallery.com
•"The Life and Mystery of Van Gogh" presented by Lucy Jerue as part of Noyes Museum Thursday Art at Night Series, 6:30 p.m. Thursday, June 28, at Noyes Museum of Art, 733 Lily Lake Road, Galloway Township, refreshments provided by Galloway Township Women's Club. Registration requested: 609-652-8848. Free to Noyes Museum members; $5 nonmembers.
•The Noyes Gallery in Hammonton presents "Fiber Revolution: Botswana Collection & Kalahari Quilts," a travelling four-year exhibition from Botswana to America, on view through Aug. 11. Go to www.noyesmuseum.org/
• Art in the Park takes place 3 to 8 p.m. fourth Fridays through September on the beach behind Ventnor Library, 6500 Atlantic Ave., Ventnor. Regional artists demonstrate, discuss and sell their work. Free arts and crafts booths for children, puppet shows, dance and music presentations.
•Currently on exhibit at The Noyes Museum of Art of Richard Stockton College of New Jersey, 733 Lily Lake Road, in the Oceanville section of Galloway Township: "Water: Illusions of Purity - Kate Dodd," on view through through Aug. 26; "Enduring Brilliance: The Pastel Society of America at 40," on view through Sept. 2; "Toylanders," through Sept. 2; New Jersey State Council on the Arts Fellowship Exhibition, on view through Sept. 9; "Stan Sperlak: Into the Night," a solo exhibition of new pastel work, on view through Sept. 23. Hours are 10 a.m. to 4:30 p.m. Mondays through Saturdays, with extended hours until 8 p.m. Thursdays; and noon to 5 p.m. Sundays. Admission fees: $5 adults, $4 students and seniors. Call 609-652-8848 or go to www.noyesmuseum.org
•The Summer Fine Arts Festival hosted by the Brigantine Beach Cultural Arts Commission and the Brigantine Historical Society takes place through July 15 at the Historical Museum. Contact Joanne at 609-266-2806 or firstname.lastname@example.com
•"Alley Art: Discovering Wabi Sabi" will be on exhibit at Great Bay Gallery, 829 Bay Ave., Somers Point, from July 25 through Sept. 1. A "Meet the Artist" reception will be held from 5 to 8 p.m. Aug. 3. The event is free and open to the public. Call 609-653-4991 or go to www.greatbayartgallery.com
•The exhibition "Works on Paper," is in residence at the Richard Stockton College of New Jersey Art Gallery through Aug. 16. The exhibit is from the Noyes Museum of Art's Permanent Collection and features 45 artists in a mix of intaglio, lithography, digital, serigraphy, chine colle, painting, handmade paper and mixed media. The gallery is open to the public from noon to 4 p.m. Mondays through Thursdays. Admission is free. It is located adjacent to the Performing Arts Center on Stockton's main campus in Galloway Township. Call 609-652-4566.
•Nationally known watercolor artist Donna Elias will exhibit her "Great American Lighthouses Collection" and her "By the Seaside Series" in the Crafter's Village in Historic Gardner's Basin Maritime Park, 800 North New Hampshire Ave., Atlantic City. Admission is free. Elias' shops are open 10 a.m. to 7 p.m. Thursdays through Sundays. Call 609-348-5826.
•"Examining Themes of Racial Stereotyping: Black Sambo, Aunt Jemima, the Gold Dust Twins, Amos & Andy and Buckwheat," currently on display at the African American Heritage Museum of Southern New Jersey, Martin Luther King Center, 661 Jackson Road, Newtonville, free admission, donations accepted. The exhibit will become a permanent display. Hours are 10 a.m. to 4 p.m. Tuesdays to Saturdays; closed Sundays and Mondays. Call 609-704-5495 or go to www.aahmsng.org
Cape May County
•The William Ris Gallery in Stone Harbor opens a two-woman show on Saturday, June 30: Jane Hartley's "Knot Play" and Julia Sutliff's "Outside in Nature." The gallery is located at 9400 Second Ave. Call 609-368-6361 or go to www.williamris.com
•"Arts on Asbury," an outdoor gallery art sale hosted by the Ocean City Fine Arts League, takes place from 10 a.m. to 2 p.m. July 15 at the gallery, 608 Asbury Ave., Ocean City. Rain date is July 23. All proceeds support the OC Fine Arts League and local artists. Call 609-814-0308 or go to oceancityfineartsleague.org
•The Lower Township Library will feature fiber works by Mary Stewart during the month of July. The library is located at 2600 Bayshore Road, in the Villas. Hours are 9 a.m. to 8 p.m. Mondays, Wednesdays and Thursdays; 9 a.m. to 5 p.m. Tuesdays and Fridays; and 9 a.m. to 4:30 p.m. Saturdays. Call 609-886-8999.
•Cape May Artists Cooperative member Diana Cutshall will demonstrate beading techniques from 4 to 6 p.m. June 30 outside the gallery at the West End Garage, 484 West Perry St., Cape May. The focus will be on beading techniques used by various African tribes, particularly the Maasai of Northern Tanzania and Samburu of Northern Kenya. Call 609-770-8261.
•The Art of Graham Nash exhibition can be seen at Ocean Galleries, 9618 Third Ave., Stone Harbor, June 28 to July 8. Nash will be at the gallery signing purchased artwork from 7 to 10 p.m. July 1.
•"Sails & Sterns," the June exhibit at Harbor Gallery, located at the Nature Center of Cape May, 1600 Delaware Ave., Cape May, features local artists Don Merwin and Janet McShain. All artwork is for sale, with a portion supporting the Nature Center. Call 609-884-2736.
•Sculpture by South Jersey's Katherine Stanek, watercolors by Marcie Cooperman and oils by Dori Spector are on exhibit through summer at William Ris Gallery, 9400 Second Ave., Stone Harbor. Call 609-368-6361 or go to www.williamris.com
•The first annual GLBT Artist Exhibit, by GABLES of Cape May County, can be viewed 1 to 4 p.m. to the end of June, at The Beesley House, 605 Route 9, Cape May Courthouse. Call 609-463-6370 or visit www.gablescapemay.com
•The Ocean City Fine Arts League's June flower-themed exhibit on display at the Art on Asbury Gallery, 608 Asbury Ave., Ocean City. Gallery hours are 10 a.m. to 5 p.m. Monday through Saturday, and 10 a.m. to 1:30 p.m. Sunday, through the summer. Call 609-814-0308 or visit oceancityfineartsleague.org
•Rosalie Hettenbach is the featured artist in the newly renovated A Ca Mia restaurant, 524 Washington Street Mall in Cape May. Her exhibit of 29 oil paintings is on display through December. The public is invited to meet the artist at a free reception on Sunday, July 8, from 1 to 3 p.m. Call 609-465-6008.
•SOMA NewArt Gallery presents three solo exhibits - Sean Taylor's "The Boxer and The Beauty Queen," Harriett Sosson's "On the Way to Cape May," and John Borrero's "Mythology, Fables and Fairy Tales," to July 29. The gallery is located at 31 Perry St., in Carpenters Square Mall, Cape May. Summer hours are 10 a.m. to 10 p.m. daily. For more information call 609-898-7488 or go to www.somagallery.net
•"Sheet Music Covers, A Decorative Art," graphics and artwork from World War I- and World War II-era sheet music covers, at Naval Air Station Wildwood (NASW) Aviation Museum, Historic Hangar No. 1, Cape May Airport, 500 Forrestal Road. Hours are 9 a.m. to 4 p.m. Mondays through Fridays. $10 adults, $8 children, free for age 3 and younger. Call 609-886-8787.
Cumberland County and West
•To commemorate the 50th anniversary of the American Studio Glass Movement, WheatonArts will present "Celebration: 50 / Forward," on Friday, Saturday and Sunday, June 29, 30 and July 1. Glass Studio events and presentations are open to the public with general admission to WheatonArts. The weekend schedule of activities in the Glass Studio are: Friday, June 29 - 9 a.m. to 5 p.m., Studio Projects/Artist Demonstrations; Saturday, June 30 - 9 a.m. to noon, Studio Projects/Artist Demonstrations; 12:30 to 1:45 p.m., Presentation: Toledo Workshop Revisited with Andrew Page, Glass Magazine editor, and Robert Minkoff from the Robert M. Minkoff Foundation; 2 to 5 p.m., Studio Projects/Artist Demonstrations; Sunday, July 1 - 10 to 11:30 a.m., Artist Roundtable with moderator Andrew Page, Glass Magazine editor; noon to 5 p.m., Studio Projects/Artist Demonstrations. Call 800-998-4552 or 856-825-6800 or www.wheatonarts.org
•The works of Bridgeton artist Kurt Hansen can be seen in a front window of the Ashley McCormick Building, located at 40 East Commerce St., Bridgeton. The collection changes monthly, and all works are for purchase. Contact the artist at email@example.com.
Ocean County
•Danny Ng is the Artist of the Month for June at Pine Shores Art Association. The public is invited to see his works in acrylic, watercolor, oil and pastel in the Pine Shores gallery throughout the month 1 to 3 p.m. Sundays, 7 to 9 p.m. Mondays, and 11 a.m. to 2:30 p.m. Wednesdays. Go to www.pineshoresartassociation.org or call 240-462-8963.
•Pat Shepherd, Marilyn Flagler and Suzi Hoffman, all members of the Pine Shores Art Association, will have their works displayed in the Stafford Library through June. For hours and information call 609-693-9351.
•The Stafford branch of the Ocean County Library features teen artwork from July 2 to Aug. 8. The library is located at 129 N. Main St., Manahawkin. Call 609-597-3381 or go to www.theoceancountylibrary.org
•Pine Shores Art Association, 94 Stafford Ave., Manahawkin, weekly open studio sessions: Sundays: Portraits, 1 to 3 p.m.; Mondays: Portraits, 7 to 9 p.m.; Tuesdays: Artists Helping Artists, 7 to 9 p.m.; Wednesdays: "Do Your Own Thing" 10 a.m. to 3 p.m.; Thursdays: Life Drawing, 7 to 9 p.m. Call 609-597-3557 or www.pineshoresartassociation.org
•The Pine Shores Art Association summer fundraising event will be an exhibit of 50 framed, original works of art donated by 50 artists. Each painting will be offered for sale at only $50 to the first 50 people. Art patrons will be able to select their favorite work in an order chosen through a lottery drawing at a gala reception beginning 1 pm. July 22 at Stafford Firehouse, 133 Stafford Ave., Manahawkin. Art Patron forms are available at www.pineshoresartassociation.org
View complete listings www.PressofAC.com/AtTheShore.
Items for Art Gallery should be sent two weeks in advance to email@example.com or mailed to Lucia C. Drake, The Press of Atlantic City, 11 Devins Lane, Pleasantville, N.J. 08232.


text:
Dr. Kevin Berman MD Patient Reviews
- Dermatology Roswell, GA
Overall Rating
30 Patient Reviews
- Wait Time 16 minutes
- Easy Appointments
- Promptness
- Friendly Staff
- Fair and Accurate Diagnosis
- Bedside Manner
- Spends Time With Patients
- Appropriate Followup
Showing 1 - 12 reviews
5.0 of 5
February 4th, 2016
Excellent Dr.
Great Dr. , very pleasant , very thorough. Great personality .
5.0 of 5
February 2nd, 2016
Doctor Berman shows passion for his work, and listens to patient. He diagnosed my sunburn and treated it effectively.
5.0 of 5
August 24th, 2015
A comfortable visit in all aspects. Such a pleasant gentlemen!
1.0 of 5
April 22nd, 2015
Did a 5-10 min exam on my son and never said anything about a mole he had and they told him the rash he had under his arm was an allergic reaction and told him and his parents worry too much. Ended up getting a second opinion and they did a biopsy on his mole, as well as diagnosis his rashed, prescribed him medicine and now it is gone. This also happened to my husband. My son's mole came back fine, but my husband's was cancerous. They are not very thorough at this practice. I also had an exam here and now I'm wondering if they overlooked something on my. We will not be going back to this practice again!
5.0 of 5
April 17th, 2015
SO happy and impressed with everything about this office!!!!
5.0 of 5
August 6th, 2013
Coming Location is the best!
I absolutely love Dr. Berman. I've seen him before at the Roswell location, but recently have been making appointments in Coming due to the appointment availability. I've been to other dermatologists within the ATL Center for Derm Diseases, but Dr. Berman is by far my favorite. He truly cares about your skin and your well being, which I'm finding so hard to come by these days. He makes the appointment quick and efficient, yet effective, and always answers any questions I have. The Coming location not only is easier to make appointments at, but the staff there is SO wonderful. I wouldn't suggest any other doctor or office! Also, I realize that this is not the proper way to spell the city's name, but vitals.com considers it's name "profanity" and won't let me post my review otherwise.
5.0 of 5
November 7th, 2012
Dr. Berman is one of the better Derms I've every seen
Dr. Berman really looks our for his patients and unlike some other Dermatologists I've seen in the past, doesn't seem annoyed by your questions or telephone calls. I was so surprised when he returned my after hours call right away and seemed genuinely concerned instead of genuinely bothered. His level of awareness regarding prescribing medication is very high as he always points out the cost to me and offers coupons if he has them. I am not currently insured so this is a huge help to me. I use to see a different Dermatologist who didn't think twice about give me prescriptions for medications that were over $200 (for a single med) when a different, equally effective med would be $20-$30.His office staff is extremely friendly and very patient.
1.0 of 5
August 9th, 2011
After waiting for an hour I left his office never to return. I have seen him 4 times and each time I waited 30 to 45 minutes. All the other doctors seem to be able to schedule appointments well, but his patients are stacked up in the waiting room. Very Poor!!
5.0 of 5
May 25th, 2017
5.0 of 5
May 25th, 2017
5.0 of 5
May 24th, 2017
5.0 of 5
May 24th, 2017
Health Guides
Get the important facts about acne, including the different types and what causes it.
more health guides
Nearby Doctors
Dr. Kevin Berman, MD is similar to these 3 Doctors near Roswell, GA.
Roswell, GA
Roswell, GA
Roswell, GA


text:
Fully-insured shipping: $15.95
The Manfrotto LW-99 PL Rolling Organizer Case is an extra-large rolling organizer built to provide the most lightweight high-protection storage and transportation solution for lighting gear. Designed to hold a variety of video or photo lighting kits, this case will withstand harsh use in any environment.
Interchangeable Dividers
Both modular and easy to use, these dividers allow you to custom fit the inside of the LW-99 PL around your gear by creating the exact size compartment for your gear.
Water Repellent
The LW-99 PL uses specially applied coating to the fabric which keeps resistant to moisture.
Camera Protection System
Manfrotto's CPS – Camera Protection System has been specifically engineered and laboratory tested to guarantee the highest level of protection and shock resistance where it really counts.
|Internal main compartment height
|108 cm
|Internal main compartment length
|40 cm
|Internal main compartment width
|24 cm
|External height
|114 cm
|External length
|45 cm
|External width
|30 cm
|6.52 kg


text:
Curse of the witching tree 2015 full movie watch online. An innocent woman, accused of murdering her son and hanged as a witch, curses a tree and the children who play around it. The effects of this act of revenge echo through the years …..
If you're unable to stream the movie, Please refresh the page by pressing F5 button and try to play the movie!
Published on April 19, 2016 by admin