text:
RNA-seq analysis of differential gene expression in liver from lactating dairy cows divergent in negative energy balance
The liver is central to most economically important metabolic processes in cattle. However, the changes in expression of genes that drive these processes remain incompletely characterised. RNA-seq is the new gold standard for whole transcriptome analysis but so far there are no reports of its application to analysis of differential gene expression in cattle liver. We used RNA-seq to study differences in expression profiles of hepatic genes and their associated pathways in individual cattle in either mild negative energy balance (MNEB) or severe negative energy balance (SNEB). NEB is an imbalance between energy intake and energy requirements for lactation and body maintenance. This aberrant metabolic state affects high-yielding dairy cows after calving and is of considerable economic importance because of its negative impact on fertility and health in dairy herds. Analysis of changes in hepatic gene expression in SNEB animals will increase our understanding of NEB and contribute to the development of strategies to circumvent it.
RNA-seq analysis was carried out on total RNA from liver from early post partum Holstein Friesian cows in MNEB (n = 5) and SNEB (n = 6). 12,833 genes were deemed to be expressed (>4 reads per gene per animal), 413 of which were shown to be statistically significantly differentially expressed (SDE) at a false discovery rate (FDR) of 0.1% and 200 of which were SDE (FDR of 0.1%) with a ≥2-fold change between MNEB and SNEB animals. GOseq/KEGG pathway analysis showed that SDE genes with ≥2- fold change were associated (
RNA-seq analysis showed that the major changes at the level of transcription in the liver of SNEB cows were related to fat metabolism. 'Steroid hormone biosynthesis', a process that normally occurs in reproductive tissue, was significantly associated with changes in gene expression in the liver of SNEB cows. Changes in gene expression were found in this pathway that have not been previously been identified in SNEB cows.
Selective breeding for high milk yield in dairy cows has led to breeds in which the nutritional demands of the very high lactation rates following calving are in excess of that which the animal can metabolise from ingested feed
The principal physiological response of animals to NEB is to try and maintain homeostasis by mobilising body fat (and protein) reserves. This results in the release of non-esterified fatty acids (NEFAs) from adipose. NEFAs are transported primarily to the liver where they are either fully oxidized to CO2, or converted to ketone bodies such as beta-hydroxybutyrate (BHB), or esterified into triacylglycerides (TAG) either for delivery into blood as very low density lipoproteins proteins (VLDL) or storage as cytosolic lipid droplets
As with the majority of metabolic states, changes in gene expression in the liver are central to NEB. There have been several studies describing such changes at the level of transcription in the liver of NEB cows which have employed qPCR
RNA-seq is a relatively new technique that can be used to analyse changes in gene expression across the entire transcriptome
Unlike microarrays, the bioinformatic tools for the analysis RNA-seq data are still at the early stages of development
Here we describe the use of DEseq
Results and discussion
Library preparation and sequencing of polyA mRNA-seq libraries
Twelve polyA RNA-seq single read libraries that had been prepared from total RNA extracted from liver from 6 MNEB animals (libraries 1–6) and 6 SNEB animals (libraries 7–12) were run as 36 bp single reads on 21 lanes randomly distributed across 3 flowcells (Table
Additional file 1
Table of number and assignment of reads for each flowcell lane.
Click here for file
Transcriptional profile of cattle liver
The lowest limit of detection was set to 5 or more uniquely aligned reads in at least one animal in either the SNEB or MNEB group. At this limit, 12,523 and 12,833 genes were detected as expressed in MNEB and SNEB animals respectively. This is similar to the number of human Ensembl genes detected in human (12,191) and mouse (11,201) liver tissue by polyA RNA-seq
Figure 1
Complexity of NEB cattle liver transcriptome
Complexity of NEB cattle liver transcriptome. (A) Average number of reads across 21 flowcell lanes aligning to genes. (B) Average FPKM values for genes across 21 flowcell lanes.
Lane effect
To further determine whether the differential expression observed between MNEB and SNEB was due to a real difference between the groups and not simply random variation caused by running samples on different lanes (i.e. lane effect), correlation analysis was performed between FPKM values from the two treatment groups and also the same 9 libraries run as technical replicates on two different lanes (Figure
Figure 2
Lane effect
Lane effect. (A) Average FPKM values for each gene in SNEB animals correlated with average FPKM values for each gene in MNEB animals. (B) Average FPKM values for each gene for libraries 1, 2, 5, 6, 7, 10, 11, 12 run on lanes 1A, 3B, 3A, 2B, 4B, 7A, 7B, 1B respectively correlated with average FPKM values for each gene for libraries 1, 2, 5, 6, 7, 10, 11, 12 run on lanes 2A, 1C, 4A, 3C, 4C, 8A, 5A, 7B, 8C respectively.
Identification of SDE genes
DEseq is an R-based software package that was developed specifically for the identification of SDE genes from raw counts of sequence reads generated by RNA-seq analysis that uniquely align to genes. RNA-seq library 1 (corresponding to MNEB animal 1) was not included in these analyses as this animal subsequently became ill and a possible underlying infection may have skewed the gene expression data generated. DEseq analysis showed that 413 genes were SDE (FDR of 0.1%), 201 of which had a fold change of ≥2 (either up or down) between MNEB and SNEB animals (Additional file
Additional file 2
Table SDE genes and their fold-change.
Click here for file
Comparison of microarray and RNA-seq
We previously analysed the same MNEB and SNEB liver RNA samples using the Affymetrix 23 K bovine gene microarray platform and, of the 5,229 genes which were detected as expressed, 416 were identified as SDE by the puma method using the recommended cut-off P-like value of
Additional file 3
Comparison of microarray and RNA-seq SDE genes.
Click here for file
Physiology of SNEB model
Soon after calving in high-yielding dairy cows, nutritional and energetic demands can increase 4-fold within a single day as the animal undergoes a physiological upheaval to meet the demand by the mammary gland for substrates, particularly glucose, for milk production. The main changes of this upheaval are a reduction of insulin concentration in the blood, a reduction of lipogenesis in adipocytes, an increase in export of fats from adipose to liver, and a dramatic increase in gluconeogenesis in liver. Unlike non-ruminants, where glucose is absorbed via the small intestine, approximately 90% of all glucose in ruminants is synthesised by gluconeogenesis in the liver from volatile fatty acids (VFAs) derived from bacterial fermentation in the rumen. VFAs are absorbed across the rumen wall and into the hepatic portal vein which delivers them to the liver. The increase in hepatic gluconeogeneis at the onset of lactation leads to production of very high quantities of glucose. The glucose entry into the blood stream in top performing cows producing 90 kg milk per day has been estimated at 7.4 kg per day of which 4.4 kg will end up as lactose in milk
In our model we were comparing two groups of lactating animals in the early post-calving period (13 or 14 days post-calving). One of these groups was in MNEB and the other was in a state of SNEB which was artificially induced by restricting feed and trebling the milking frequency. From this we hoped to identify novel genes and pathways which are important in SNEB in high-yielding dairy cows. Hormones, metabolites and aspects of liver composition measured at the time of tissue collection showed that, as predicted, relative to MNEB cows, SNEB cows had undergone significant (
Additional file 4
Graph of effect of SNEB compared to MNEB on blood concentrations of major NEB associated metabolites 1–14 days post-calving.
Click here for file
Over-represented KEGG pathways
In our RNA-seq study, nine KEGG pathways were associated with ≥2-fold SDE genes most of these were related to metabolism of fats (Table
Additional file 5
Maps of KEGG pathways (associated with ≥2 fold (FDR 0.1%) SDE genes) overrepresented in SNEB animals.
Click here for file
Additional file 6
Maps of KEGG pathways associated with all SDE (FDR 0.1%) genes.
Click here for file
Figure 3
Changes in hepatic gene expression in the KEGG PPAR signalling pathway in SNEB compared to MNEB cows
Changes in hepatic gene expression in the KEGG PPAR signalling pathway in SNEB compared to MNEB cows. Blue boxes are genes detected by RNA-seq as expressed but not SDE, red boxes are SDE genes with reduced expression in SNEB animals, green boxes are SDE genes with increased expression in SNEB animals.
Figure 4
Changes in hepatic gene expression in the KEGG Steroid hormone biosynthesis pathway in SNEB compared to MNEB cows.
Changes in hepatic gene expression in the KEGG Steroid hormone biosynthesis pathway in SNEB compared to MNEB cows. Blue boxes are KEGG enzymes coded for by genes detected by RNA-seq as expressed but not SDE, red boxes are KEGG enzymes coded for by SDE genes with reduced expression in SNEB animals, green boxes are KEGG enzymes coded for by SDE genes with increased expression in SNEB animals. KEGG enzyme 18.104.22.168 is coded for by HSA CYP11A1, KEGG enzyme 220.127.116.11 is coded for by HSA
Carbohydrate metabolism
The 1.5-fold drop in blood glucose in SNEB cows would suggest decreased gluconeogenesis in the liver of these animals. However, only a minor decrease (≤2-fold) of pyruvate carboxylase (PYK (KEGG pathway map) =
Uncoupling of the GH/IGF1 axis
Although pathways related to the GH/IGF1 axis were not identified as overrepresented among the SDE genes identified by RNA-seq, several genes within in this pathway were detected as SDE (
TAG accumulation and increased blood NEFA
Increased TAG accumulation in the liver was reflected by overrepresentation of the KEGG 'PPAR signalling pathway' (Table
Oxidation of NEFAs and ketogenesis
Due to the higher amount of NEFAs in the blood of SNEB animals, we were expecting changes in expression of genes to reflect increased oxidation of fatty acids in SNEB animals. However, although eight of the nine fatty acid oxidation genes in the KEGG PPAR signalling pathway were detected, none were SDE (Additional file
Impaired PUFA synthesis
The most SDE gene we identified by RNA-seq was
Unexpected overrepresentation of KEGG pathway 'steroid hormone biosynthesis'
One of the most interesting findings of our RNA-seq study was that the KEGG pathway 'Steroid hormone biosynthesis' (Table
CYP11A1, a novel gene in metabolic adaptations to post-partum bovine SNEB
Although we detected upregulation of
RNA-seq analysis showed that the major changes at the level of transcription in the liver of SNEB cows were related to fat metabolism. Unexpectedly, one of the most significantly changed biological pathways was 'Steroid hormone biosynthesis' where
Materials and methods
Animal model
The animal model employed in this study has been described previously
Collection of liver tissue for RNA and TAG analysis
The entire liver was removed within 15–30 min after slaughter and weighed. Samples weighing approximately 1 g were dissected, rinsed in RNase-free phosphate buffer, snap-frozen in liquid nitrogen, and stored at −80°C. For triacylglyceride (TAG) analysis, total lipids were extracted from 50 mg samples of liver as previously described
Blood sampling and metabolite assays
Stabilized (EDTA-treated) whole blood samples were collected on the day of slaughter by jugular venipuncture for haematological analysis. Blood samples were analyzed for glucose, NEFA, β-hydroxybutyrates (BHB), and urea using appropriate kits and an ABX Mira autoanalyser (ABX Mira, Cedex, France). All metabolite assay coefficients of variation were low and typically <5%.
RNA extraction and quality analysis
Total RNA was prepared from 100–200 mg of fragmented frozen liver tissue using the TRIzol reagent (Sigma-Aldrich, Dorset, UK). Tissue samples were homogenized in 3 ml of TRIzol reagent and chloroform and subsequently precipitated using isopropanol (Sigma Chemical, Wicklow, Ireland). RNA samples were stored at −80°C. 20 μg of total RNA from each sample for genomic DNA contamination was treated with the RNase-free DNase set (QIAGEN, Crawley, West Sussex, UK) and purified it using the RNeasy mini kit in accordance with guidelines supplied (QIAGEN, Crawley, West Sussex, UK). RNA quality and quantity were assessed using automated capillary gel electrophoresis on a Bioanalyzer 2100 with RNA 6000 Nano Labchips according to manufacturer's instructions (Agilent Technologies Ireland, Dublin, Ireland). Samples of RNA had 28 S/18 S ratios ranging from 1.8 to 2.0 and RNA integrity number values of between 8.0 and 10.0.
Preparation and sequencing of polyA mRNA-seq ilumina libraries
polyA RNA was isolated from 5–10 ug total RNA with oligo(dT) beads using two rounds of oligo-dT purification. 5–10 ug RNA was fragmented with zinc fragmentase (Applied Biosystems, Warrington, UK), first strand cDNA synthesis was performed using the Invitrogen random hexamer primers and SuperScript II (Invitrogen), second strand synthesis was performed using Invitrogen DNA Polymerase 1 (Invitrogen). End repair and polyadenylation were performed using NEB Next Tailing Module (New England Biolabs). ® End Repair Module and NEB Next dA- Illumina single read adapters were ligated to blunt ended, polyadenylated fragments with a NEB Quick ligation kit (New England Biolabs). Adapter-ligated cDNA fragment libraries were run on an Illumina GAII using version 3 sequencing kits and version 3 single read cluster generation kits.
Read alignment
The reads from each of the lanes were aligned separately to the BCM4 genome assembly
Read abundance calculation
The abundance of mRNAs for all annotated genes from the ENSEMBL v59 annotation of the bovine genome
Identification of SDE genes and pathway analysis
We identified SDE using DEseq (version 1.1.11)
GOseq and KEGG pathway analysis tools were used to identify biological pathways that were significantly enriched in the data set of SDE genes (Table
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
MSM generated the RNA-seq libraries, co-ordinated the sequencing runs, helped with retrieval and analysis of data, carried out detailed interpretation of the pathway analysis, and prepared the main manuscript. SW extracted the RNA from NEB liver samples, conceived the idea of performing RNA-seq on the NEB RNA samples and contributed to the biological interpretation of the data and manuscript preparation. DL contributed to the bioinformatics of differential gene expression analysis and pathway analysis and writing and editing of the manuscript. DK, and DM co-ordinated tissue collection and RNA extraction from the NEB model, conducted metabolite studies on the model and contributed to the biological interpretation of the data and writing and editing of the manuscript. CC set up and performed the majority of the bioinformatics on the sequencing runs including data retrieval, read alignments, differential gene expression analysis, KEGG pathway analysis, data interpretation and co-wrote the main manuscript with MSM. All authors read and approved the final manuscript.
This work was funded by Teagasc under the Irish National Development Plan and Chris Creevey is funded under the Science Foundation Ireland (SFI) Stokes lecturer scheme (07/SK/B1236A). The authors thank Alison Murphy and Amanda Lohan for their technical support and advice.
Negative energy balance alters global gene expression and immune responses in the uterus of postpartum dairy cows
ADSA Foundation Scholar Award. Biology of dairy cows during the transition period: the final frontier?
A species comparison of liver slice synthesis and secretion of triacylglycerol from nonesterified fatty-acids in media
Splanchnic metabolism of dairy cows during the transition from late gestation through early lactation
Pleiotropic effects of negative energy balance in the postpartum dairy cow on splenic gene expression: repercussions for innate and adaptive immunity
Effects of propylene glycol or fat drench on plasma metabolites, liver composition, and production of dairy cows during the periparturient period
Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow
Genomics of metabolic adaptations in the peripartal cow
Interrelationships between negative energy balance (NEB) and IGF regulation in liver of lactating dairy cows
Nutrition-induced ketosis alters metabolic and signalling gene networks in liver of periparturient dairy cows
Negative energy balance and hepatic gene expression patterns in high-yielding dairy cows during the early postpartum period: a global approach
Comparison of transcriptomic landscapes of bovine embryos using RNA-Seq
Mapping and quantifying mammalian transcriptomes by RNA-Seq
RNA-Seq: a revolutionary tool for transcriptomics
Widespread transcription at neuronal activity-regulated enhancers
A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
(2010) SNP discovery in the bovine milk transcriptome using RNA-Seq technology
Chimeric transcript discovery by paired-end transcriptome sequencing
From RNA-seq reads to differential expression results
Computational methods for transcriptome annotation and quantification using RNA-seq
Differential expression analysis for sequence count data
Gene ontology analysis for RNA-seq: accounting for selection bias
The genome sequence of taurine cattle: a window to ruminant biology and evolution
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
An abundance of ubiquitously expressed genes revealed by tissue transcriptome sequence data
Gluconeogenesis in dairy cows: the secret of making sweet milk from sour dough
Short communication: hepatic gene expression for gluconeogenic enzymes in lactating dairy cows treated with bovine somatotropin
Tissue expression of angiopoietin-like protein 4 in cattle
The transcriptomic profiles of adipose tissues are modified by feed deprivation in lactating goats
Dietary arachidonate differentially alters desaturase-elongase pathway flux and gene expression in liver and intestine of suckling pigs
Genetic variants of the FADS1 FADS2 gene cluster as related to essential fatty acid metabolism
Delta6-desaturase (FADS2) deficiency unveils the role of omega3- and omega6-polyunsaturated fatty acids
DNA methylation is not involved in preovulatory down-regulation of CYP11A1, HSD3B1, and CYP19A1 in bovine follicles but may have a role in permanent silencing of CYP19A1 in large granulosa lutein cells
Fasting-induced hepatic production of DHEA is regulated by PGC-1alpha, ERRalpha, and HNF4alpha
Ensembl's 10th year
Controlling the false discovery rate - a practical and powerful approach to multiple testing


text:
The Watchtower of the River of the Unexpected Hero (U24) was located in the Enkaku province, a small Moto province beyond the Ide lands. [1] After construction began on the Khol Wall, the Watchtower served as the wall's southern headquarters. [2]
Appearance Edit
The Watchtower was an extremely tall fortress almost 100 feet high made of dark blue stone. Built in the middle of a vast, open field some distance north of the great Shinomen Forest. It was extremely broad at its base and narrowing only gradually. An interior staircase connected the various levels, many of which served as barracks. [3]
Garrisoned Edit
Originally, the garrison was comprised of Ide troops, but in the 12th century they were replaced. [2] An entire legion was appointed there to defend this area from any possible attack from the Shadowlands, [4] fondly nicknamed the Unexpected Legion. A samurai stationed at the watchtower could see for miles on a clear day. In the event of a Shadowlands assault there were more Unicorn samurai stationed here than any other fortification. They were heavily armed and drilled and trained constantly, leaving the lands scarred by combat maneuvers in two miles in all directions. [5]
|This Unicorn Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.

subset: commoncrawl


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Chris Martin Son Moses at Lakers Game January 2016
Chris Martin Gets in Some Quality Time With Son Moses Ahead of the Super Bowl
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Chris Martin Gets in Some Quality Time With Son Moses Ahead of the Super Bowl
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Data from: The ecology of seed dispersal by small rodents: a role for predator and conspecific scents
Date Published: August 7, 2013
DOI: http://dx.doi.org/10.5061/dryad.763bh
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text: Hi all, I've got a two-node EL5.6 cluster with a handful of interfaces on the nodes. When I do not specify MTU=x, everything works great. However, I want to use jumbo frames, and so the fun begins. As soon as I define MTU=2000 (for example), then cman on one note will start but not stop (the other node stops fine). Also, 'ccs_tool update /etc/cluster/cluster.conf' fails with: ==== [root xenmaster003 ~]# ccs_tool update /etc/cluster/cluster.confFailed to receive COMM_UPDATE_NOTICE_ACK from xenmaster004.iplink.net. Hint: Check the log on xenmaster004.iplink.net for reason. Failed to update config file. ==== Nothing at all gets written to the target node's log files though. Is there some subtle magic needed to get jumbo frames working in the cluster? Am I missing the forest for the trees? :) -- Digimer E-Mail: digimer alteeve com AN!Whitepapers: http://alteeve.com Node Assassin: http://nodeassassin.org


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By Lorenzo G. March 30, 2017
La moda degli Ultrabook sempre più sottili e compatti ha portato le case a "tagliare" qualche componente presente nei loro dispositivi, uno di questi è la porta Ethernet, che è sempre più difficile da trovare a bordo dei più recenti PC portatili. Prima di partire per Barcellona per il MWC17 son dovuto correre ai ripari per poter risolvere questo "problema" sul mio Asus UX303UB e grazie a Dodocool ho trovato la mia soluzione. Oggi vi parlo di Dodocool DC18-1 Hub Usb 3.0 + Porta Ethernet Gigabit dalle dimensioni compatte e dal design moderno. -Confezione di vendita- La confezione di vendita è molto minimale, ma giustamente stiamo parlando di un "semplice" Hub Usb. L'imballo di vendita è un semplice cartonato, sulla parte frontale troviamo la foto del dispositivo presente all'interno e le caratteristiche tecniche. All'interno troviamo l'Hub Usb 3.0 e il classico libretto delle istruzioni dove vengono riportare le carattristiche tecniche e la modalità d'uso, il tutto scritto in 6 lingue. -Design e materiali- Il design, nonostante si stia parlando di un "semplice" Hub Usb, è moderno e ben curato, con linee morbide. Questo Dodocool DC18-1 è costruito interamente in plastica, con una lavorazione ruvida leggermente antiscivolo, nella parte frontale, oltre alle 3 porte USB 3.0, troviamo un led di notifica di collegamento al pc. Molto utili i 4 gommini antiscivolo posti nella parte inferiore dell'Hub che garantiscono un'ottima presa su tutti i tipi di piani. Il cavo ha una copertura in pvc non troppo spessa, ma abbastanza resistente. -Le nostre considerazioni- La tendenza, tra le case costruttrici, è quella di produrre Ultrabook sempre più compatti e leggeri e spesso bisogna correre a compromessi e tagliare magari certi componenti, come porta Usb o porte Ethernet. Questo Dodocool DC18-1 viene in soccorso di chi, come noi, è sprovvisto di porte Ethernet o ha bisogno di qualche porta Usb in più sul proprio Ultrabook o PC. Noi abbiamo optato su questo Dodocool DC18-1 per le sue dimensioni compatte e portatili ed anche per il suo design minimale ma per niente anonimo. Ci ha accompagnato nei 4 giorni stati a Barcellona e possiamo dire che ha fatto egregiamente il suo dovere, non abbiamo avuto alcun tipo di problema, il collegamento con la porta Ethernet è sempre risultato stabile senza problemi di connessione e le 3 porte Usb hanno retto egregiamente lo "stress" giornalistico. L'Hub è dotato anche di un led di stato, che segnala il collegamento al PC, molto utile per capire, in caso di problemi, se il problema stesso è dovuto dall'Hub o dalla porta Usb del PC. Da segnalare che questo Hub Usb non ha alcun bisogno di installazione di software, quindi alla prima connessione sarà già completamente funzionante. Chiudiamo nel dirvi che, se siete alla ricerca di un Hub compatto, con un design moderno e sopratutto affidabile, beh Dodocool DC18-1 potrebbe essere la vostra risposta e sicuramente non ve ne pentirete.
By Rick 'The Best of Everything' March 9, 2017
Dodocool USB 3.0 3Prt HUB with Gigabit Ethernet B00XW5VEO6 With the price and performance this is just an indespencible item for the tool kit. I had this one about a week and a friend had a fail in her internet connection. So, I ran over and plugged this into her USB and in a few seconds she was back online. As it turned out a noname ethernet card went toes up. So, I left the DC18 there connected to her old HP's Lone previously unnoticed USB3.0 jack on the back of the chassis so she could keep working while arranging for a replacement ethernet card. The DC18 apparently worked better than the 5 year old 10/100 card. Sort of a vague description, but if she is happy that is what counts. With thunder and lightning, you never know. Best to have some parts around in case of tragedy. And this is a handy stop gap measure. With three USB ports on the Hub it is hard to beat the convenience. The pigtail cable is just about right to reach around the back of the chassis and is just right for those annoying wired addons like keyboard, mouse, external drive. ==--> Product sample provided for fair review MDL#: DC18 ASIN: B00XW5VEO6
By Schlep March 9, 2017
This thing is absolutely perfect for someone that cannot plug an RJ-45 into their PC for Internet connectivity. I stuck this into a USB 2.0 on my laptop and the driver install came up right away and did not lake long at all to finish and I was on the Internet that fast. For those that are really clueless, you do have to have Internet access from a provider with a CAT 5 like to plug into this device. It is not magically going to give you Internet for free. That being said, I also attached two external devices to the USB 3.0 slots onto this device, a Kingston USB 2.0 flash drive and a Transcend USB 3.0 card reader. I put pics of each ready to open files and also the device itself this these things plugged in. You really cannot have it simpler than this if you do not have a port to plug the RJ-45 into. I received this at a discount in exchange for an honest review.
By PoisonPinhead March 9, 2017
By Alex March 9, 2017
By G. L. Wright March 8, 2017
By Simmons March 8, 2017
By Bob March 8, 2017
Tested with Windows 7 & Windows 8. I wanted this but my first thoughts were why anyone else would need this as I have yet to find a recent PC/laptop that does not have an Ethernet connection. This one is a Gigabit one that will handle speeds up to the limit of the usb 3 connection speed. Compatibility is claimed from XP upwards although again not many PCs with XP will have usb 3 and so not be able to make use of the additional speed. HOWEVER It is a well-built usb 3 hub, with 3 outputs so will give an additional 2 usb sockets. I found the Ethernet socket very useful, which is why I wanted it, as I do a lot of testing with routers, switches etc and this allowed me to have a socket on the front of my PC as the included one is at the back and not easy to reach where as I have a couple of usb 3 sockets on the font so for me very useful. I tested a number of usb flash memory drives and HDDs it worked well on both usb 2 and 3 obviously with less speed with usb 2. The only downside is that it is bus powered which although useful does mean that it may not be suitable for devices that need to draw high levels of current.
By Lenz March 8, 2017
By Dan Churchill March 8, 2017
By Wilson March 8, 2017
This is a nice little charger box. It is nice and small, works well and helps charge multiple phone or devices very nicely and quickly. Have not had any problems with this charger. It does do the fast charge and hasn't been hot to the touch while charging. Quality feels good and I am very pleased with my purchase.
By TheCoolGeek (YouTube) March 8, 2017
Design The USB hub is in matte black and the quality of plastic used feel sturdy and durable. It has a dimension of 94mm x 42mm x 23mm (L x W x H) and the attached USB cable is 31cm long. On the top, it has 3 USB extension ports and at the end, it has an ethernet port. The USB hub comes neatly packaged with a driver CD, and a warranty leaflet. Feature I use this USB hub mainly with my MacBook Air when I am travelling. Not only that it expands the number of USB ports, I can also use it to connect to the internet via an ethernet cable which I previously couldn't (the MacBook Air doesn't have an ethernet port). Before I wrote this review, I have ran a number of tests with the equipments I have access to. Below are some of my findings which you may find useful: * I could charge a smartphone (e.g. iPhone 6, Samsung Galaxy S6) and have two USB memory stick in use at the same time. * I have tried charging both of the above smartphones at the same time. It worked, but the charging was slow. * It have used a Seagate 1TB portable external hard drive with it, it worked fine without extra external power. * It charges bluetooth headsets, Kindles, Kobo readers, external battery packs fine * It can charge the iPad Air 2, iPad mini, but slow. * I have tested the hub on Windows 7, 8.1, 10 and Mac OS X El Capitan, it worked fine on all of them without needing to manually install drivers. * I have tested and compared the internet speed (ping, upload and download), using the built in ethernet port in PC and Mac versus this USB network hub, at broadband speed of less than 100Mbps. There was no measurable difference. All in all, this is an excellent multi-port USB 3.0 network hub. Highly recommended. Note: I was supplied with this product in exchange for my fair and unbiased review.
By Carbajal March 8, 2017
By Hancock March 8, 2017
By Juan M. March 8, 2017
El objeto es un Hub en color negro fabricado en plástico. Para empezar decir que es muy ligero apenas pesa por el material que esta fabricado. tiene 3 puertos usb 3.0 y una toma de ethernet, esta toma es bastante útil y no todos los hubs la traen, en cuanto a los puertos usb tienen una excelente velocidad de transferencia. Me ha gustado por lo ligero que es y lo pequeño al ser compacto por lo que lo puedes llevar a cualquier lado guardado en la funda de tu ordenador. Es bastante útil y como un hub para el uso diario viene muy bien.
By Kippller Tech Reviews March 8, 2017
never thought I would have a need for a product like this, But I was completely wrong. The product is well designed, It looks slick and is very slim, The black matte plastic looks fresh and doesn't look out of place at all. The usb ports are all at the right side and they have an LED indicator. There is a LAN port also, If you are going to be using this for transferring files from an external hard drive or a USB you will not be disappointed with the read and write speeds. I also found a neat little use for this product, If you have an Xbox or Playstation you can use this hub to expand the amount of USB ports you have! It provides enough power as its USB 3.0! I used the LAN port and I did not have any lower speeds than usual so thats great! One thing I would liked to have is a longer cable but I suppose thats really not a big deal. The packaging is really nice and neat ! Looks absolutely amazing. So overall its a great product especially if you have a laptop that has a few USB ports !
By Esther March 8, 2017
Se trata de un concentrador de 3 puertos USB y un puerto RJ45 que nos permite conectar múltiples dispositivos a un ordenador al mismo tiempo o cargar 3 dispositivos a la vez. Muy eficaz, con este hub 3.0 aumentamos y optimizamos la efectividad de transmisión de información. Como bien sabemos los nuevos ordenadores a parte de venir con un limitado numero de puertos USB la mayoría vienen ya sin puerto para el RJ45, en mi caso, mis ordenadores no lo tienen y si realmente necesitas el 100% de la velocidad no te vale con la conexión por wifi. Muy buena solución, en mi caso me ha dado la vida. A parte de este uso, lo puedes usar como carga conectando el USB a un cargador. Yo he llegado a cargar 3 dispositivos y sin ninguna problema. Materiale duro, robusto y buena calidad. Muy contenta con el producto, lo recomiendo.
By FLYGUY TECH March 8, 2017
By Landry March 8, 2017
Quick delivery and works as intended! I was previously using a USB 3.0 to gigabit Ethernet adapter with my Surface Pro 3. However, I needed another USB port for my serial-to-USB adapter for configuring HP Procurve switches via putty while maintaining wired network access. This was the solution to my predicament. The product is well-built and works as advertised. I couldn't be happier!
By Joel Da Silva March 8, 2017
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Sixteen years after building York Mountain Winery, Andrew York, constructed the Farmhouse in 1898. (We dig his priorities!) The home was a comforting place for his family as they lived their dream of growing grapes and producing wine atop York Mountain.
Through the years, the Farmhouse underwent many updates, yet nothing as great as our full remodel in 2013. The original home consisted of only the center section of today's structure. The kitchen (an old cabin) was added in the early 1900s, and the great room was built in the 1970s. In 2013, we took the Farmhouse down to the studs for its most comprehensive renovation to date!
Original York Family Home
Restored Armstrong Family Home
When restoring the 115 year-old Farmhouse, we knew it would take more than a can of paint and new cabinets to breathe life back into the structure. This once abandoned house needed a major makeover to let the charm of original craftsmanship shine. To lead the restoration, we enlisted our design-savvy and in-house architect, Steve George. We knew with his guidance the completed home would reflect the historic integrity and charm of the original Farmhouse all while incorporating today's modern conveniences and amenities.
Before renovation could begin, the Farmhouse needed to be stabilized with a new foundation...no easy undertaking! Once sturdy, the house was stripped down to the studs, ridding the premise of asbestos, lead paint, and black mold. Good riddance! Removal of the walls and floors revealed a maze of haphazard electrical and plumbing, along with the fireplace's brick chimney that curved through the inside of the walls to connect to the smokestack that served the stove in the kitchen. Needless to say, we had our work cut out for us, but we were on a mission to bring this iconic Farmhouse back to life.
Looking to the past helped guide many of the visual features found throughout the finished home. To achieve our restoration goals, we hired several specialized craftsmen. Thanks to their particular trades and passions, we were able to restore many of the original windows, save the linoleum-covered wood floors, and even carve a few interior doors to match the originals.
Details were key to this project! When it came to the finishing touches, architect Steve George searched far and wide to adorn the home with reflections of the past. The light fixtures and switches along with certain furniture pieces resemble those used at the turn of the 20th century, including the wrought-iron bed frames found in each beautifully designed suite.
Be it by gazing at the Farmhouse walls lined with images of times gone by, cooking a meal in the spacious kitchen bathed in California's natural light, or by taking in the views of the mountain range that hug this magical section of west Paso Robles, this charming and historic Farmhouse is once again a place for family and friends to relish in memories of the past and dream of the future atop York Mountain.


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"I just wanted you to know that when the two pieces of flannel I recently ordered arrived, I was thrilled with the quality and everything else about them!"
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I have been a volunteer for over 25 years and it has been the most rewarding experience. The people at HAI are so wonderful and totally focussed on helping the disadvantaged.Even though they started out helping hospitalized children their mission over the years has expanded to include so much more.Recently they used ther buses to take volunteers to Rockways to help Sandy victims.
I am a new volunteer at the HAI Saturday Art Studio and I relish my time working with this group of dedicated artists who come here each week to connect through art making. I am continually blown away by the art that comes out of this nurturing and supportive environment and am so grateful to be part of this program. As a working artist I find this experience has enriched my own practice by experiencing so many new ways of thinking about art.
HAI is an excellent service organization filling the needs of so many NYC residents. I've personally had the opportunity to volunteer for a large number of their programs across all of their departments and have seen the significant impact that their services have on individual lives. Their after-school programs provide a much needed outlet for hundreds of students at schools throughout the five boroughs, giving them the opportunity not only to express themselves but to learn about new forms of expression by attending plays and performances in this culturally diverse city. Their arts workshops are probably my favorite program. They provide a Saturday Workshop with free materials to individuals with little to no training and who have often been marginalized because of mental illness. I value the time that I've spent serving with HAI, and the positive impact on communities throughout NY that I've personally witnessed as a volunteer with their services. I will undoubtedly continue to serve with them in the future.
I have been a volunteer art instructor at the HAI Saturday Art Studio for almost 3 years, and it has been one of the most rewarding experiences of my life. I often feel that the workshop participants have taught me far more than I have taught them. As an art director, graphic designer, illustrator, and teacher at Parsons the New School for Design, I have found my practice immeasurably expanded through volunteering at HAI. The enthusiasm and creativity of the participants has opened up my thinking about visual art more than I would ever have imagined. Most importantly, I feel like part of an extended family. It is an honor to be part of it.
Because I fell apart after the death of my mother in 1993, I went to a Continuing Day Treatment Program (CDT) to begin to put myself back together. Her death triggered my schizoaffective bipolar type disorder. I was severely, suicidally depressed, and unable to function. My affect was flat. All of my college buddies lived far away, so I basically had no friends. Because of the disorder I could not teach, my passion, and I had no income. I had nothing except for dreary time on my hands. But one day at CDT, we were told about HAI and of a performance that HAI had free tickets for. I think it was Ballet Hispanico. I went, and even though I could not enjoy it at that time, it did something for me. I went to an occasional event that HAI gave tickets to. In 1996, at an Intensive Psychiatric Treatment Program (IPRT), I was in an art group where I was invited to attend the HAI Art Studio--My life started again after that. I had a weekend activity. I made friends. I started doing art, I got cats for the first time in my life and I started painting cats. HAI and Cats put passion back in my life. I began exhibiting my work, and eventually began selling it as well. I now have a second career that I am passionate about, and I am an award-winning Outsider Artist of HAI where I had a solo art exhibit of 60 paintings, and have appeared in international magazines and other publications, all thanks to HAI. (I still attend HAI events.) Now I am on the Board of Directors of HAI, where I do my best to give back to HAI and to give HAI to others who would benefit from the services of HAI.
I have been a performer with HAI for over 20 years. They send us to populations that would never have the chance to hear the quality of music/drama that we can offer. And all of us work there for less than we normally work for, because we believe in their mission. They have struggled for years to serve the under-valued people of this city; the elderly, the homeless, the sick. They are a valuable and needed organization in the City of New York.
Five years ago a friend recommended HAI (Hospital Audiences) as a great venue for volunteering. Since I finally had some time to donate and to "give back", I started volunteering at HAI. My goal was to provide my services to others by facilitating their attendance at cultural events and thus enhancing their lives.
Little did I know that, thru my years of volunteering at HAI, my life would also be enhanced and enriched!
There are no words to describe my joy at watching someone in wonderment as they experience, sometimes for the first time, a cultural experience which they never would have gotten to see if not for HAI.
I hope to continue to see first- hand the benefits experienced by the community which HAI serves for many years to come.
HAI is an organization that provides opportunities for individuals with Mental Ilness and MRDD patients and others, who canmot afford to go places like the Movies ,Broadway shows ,Basketball games, etc, without HAI most of these consumers could never afford a ticket to these activities . A lot of these individuals isolates themselves , as a Social Service Worker i do my best to make sure that these consumers utilize all services provided by HAI as part of their Treatment Plan , to Learn Socialization Skills , meet new people, learning how to interact and present themselves in public , HAI is the best orginization that provides valuable intertainment to my consumers and individuals with disabilities every where ,HAI continue to do the beautiful work that you are doing with God's Blessings.
HAI has a program for blind and visually impaired people. They describe live theater and when I volunteer for example at the Radio City Christmas show and the kids go up to the describer afterwards and hug them and tell them how much the enjoyed the show, I know I am doing a great thing. Way to go HAI
HAI provides a invaluable source of entertainment and cultural education for our city's under-served and handicapped communities. A diverse array of events are offered to the members of HAI and as volunteers, we have the pleasure of providing admission to these activities, while being able to engage in a very personal and intimate setting. I have had the pleasure of enjoying dance recitals, baseball games, and community dances with our community and have shared their moments of joy and excitement. The HAI program truly makes everyone's lives a little better.


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Moonpools and Caterpillars: Hear MP3
Moonpools and Caterpillars: Hear from the album, "Lucky Dumpling" (1995) Also featured in the film, "Wish Upon a Star" starring Katherine Heigl. Nationality: ...
"Hear" Moonpools & Caterpillars MP3
From "Lucky Dumpling", 1995.
Hear by Moonpools and Caterpillars MP3
This clip is the Hear music video by Moonpools and Caterpillars done back around 1995 and a clip from the movie Wish Upon a Star where the band had a ...
Moonpools and Caterpillars - Heaven MP3
I believe in heaven and know that I should just accept it all So content with the paths that I walk on - surely I should see it all So take care and don't go to far, I will ...
Moonpools and Caterpillars/ Hear lyrics MP3
Moonpools & Caterpillars "Hear" Ethiopia Health Aid July 27, 2013 MP3
Moonpools & Caterpillars playing "Hear" at the Ethiopia Health Aid event in Downtown LA on July 27, 2013, their first show in 15 years.
Moonpools & Caterpillars - Colossal Youth MP3
I am tired of all those covers, so here you go. Enjoy and leave comments. Album: Lucky Dumpling (1995 Elektra Records) Disclaimer: Copyright goes to ...
Hear (of Moonpools and Caterpillars, by www.GuitarTutee.com) MP3
http://www.guitartutee.com Moonpools and Caterpillars Guitar Tutorial Song Acoustic Live Lyrics Chords Tabs Cover Videoke How To Play.
soon - moonpools and caterpillars MP3
Moonpools and Caterpillars - Hear (live at The Outpost) MP3
April 9, 2014 - Arrgh! Can't believe I finally saw them play live! I grew up to their songs and to finally hear those songs being played live is truly overwhelming.
Moonpools and Caterpillars: Sundays MP3
Moonpools and Caterpillars: Hear from the album, "Lucky Dumpling" (1995) Nationality: USA How long have you struggled here? How long have we tried?
Moonpools & Caterpillars - Hear (Live) MP3
Hear by Moonpools and Caterpillars @ AMBER Ultra Lounge 4/11/14 MP3
Moonpools & Caterpillars - Hear (1995) MP3
album : Lucky Dumpling (1995)
Moonpools and Caterpillars - Hear MP3
Hear - Moonpools and Caterpillars (cover) MP3
center for arts, timog.
Moonpools and Caterpillars - Hear (live in Manila) MP3
Soon by Moonpools and Caterpillars Amber Ultralounge Fort Strip, Taguig Philippines April 11, 2014.


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Global design consultancy firm IDEO is featuring a recent Johnson Leadership Programs-organized workshop on design thinking on its website as a part of the firm's current "Open IDEO" challenge. Open IDEO is an open innovation platform that seeks to bring people together from across the globe to address pressing social challenges.
Twenty Johnson students participated in the October 4, 2013, to October 5, 2013, workshop, "Leading Innovation through Design Thinking," which is highlighted on the Open IDEO blog. The intensive two-day session was offered as a part of Johnson Leadership Programs' Leadership Skills Program workshop series, which offers supplemental workshops that complement the school's leadership curriculum.
The workshop was taught by Tracy Brandenburg and Sirietta Simoncini, two faculty members in Systems Engineering at Cornell, and was designed to give the Johnson participants robust training in the complete design thinking cycle of empathize, define, ideate, prototype, and test. The students spent the two days working on Open IDEO's current challenge, which asks teams to submit their ideas on the question: "At a time when our world faces unprecedented challenges, how might we ensure that young people practice their creative confidence today so that they have a shot at becoming successful leaders tomorrow?"
Brandenburg says that she and Simoncini were so inspired by the Johnson students that she decided to share their experiences at Johnson on the Open IDEO blog. In her post, Brandenburg explains that at the workshop, she and Simoncini were both teachers and students.
"So how did we–the class professors– become the students? Because our 'teachers' gave some insightful lessons that we hadn't considered as educators. For example, one notion is to consider not just celebrating failure, but insisting on it! When asked to fail, something magical happens–students design without fear."
Interim Leadership Programs Director Ingrid Jensen says the impetus for the workshop grew from a desire to enhance leadership programming around creativity. "We know that being able to create environments that foster creativity and innovation are core competencies required of any leader in the 21st century. This workshop gave students crucial hands-on training in tools they can take to their post-MBA employers that will enable them to lead teams that need to innovate."
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Melba Downey
- 59 years old
- Date of birth: Jan 9, 1938
- Place of birth:
Bastrop, Louisiana, United States
- Date of passing: Sep 1, 1997
- Place of passing:
Mer Rouge, Louisiana, United States
|Let the memory of Melba be with us forever
This memorial website was created in memory of our loved one, Melba Downey, 59, born on January 9, 1938 and passed away on September 1, 1997. We will remember her forever.
Memorial Tributes
"No other like her. The grandest musician I ever knew and so privileged to call her my sister. I miss you more every day."
"Missing you Momma !! Keep those Angel singing in tune !!!"
"I would give anything to hear granny play the piano again and have hear meet her great-grand daughter. i love you granny"
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On Monday 04 June 2007 09:14:56 Gerry Reno wrote: > Yes, I've confirmed. I burned a second DVD, verified the checksum after > the burn, mounted the disk and made sure I could browse and load from > all disk areas, retried install with new DVD and same errors as > reported. Fedora 7 Installation is stuck because DVD/CDROM drive is not > recognized. You may need the qemu from Fedora 7 to be able to run it. -- Jesse Keating Release Engineer: Fedora
Description: PGP signature


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Nixon Showout Bi-Fold Snap Coin Wallet - Herringbone/Black
Keep cash and cards within easy reach. Complete with snap coin wallet, multiple card slots and wrapped in a wool herringbone outer, this stylish piece is one you won't want to leave home without.
- Nixon accessories
- Style name: Showout Snap Coin Wallet
- Colour: Herringbone/Black
- Design: Bi-fold
- Compartments:
- 1 x note compartment
- 4 x card slots
- 2 x storage compartments
- 1 x snap coin pocket
- Embossed logo detailing
- Materials:
- Exterior: Wool/polyurethane
- Lining: Nylon
- Dimensions (approx. cm):
- Folded: 8.5 x 10.5
- Unfolded: 21 x 10.5
- Country of origin: China
Truly stylish and classic designs are hard to come by. Nixon products are created using only the highest quality craftsmanship and finest materials available. For fashion-forward deals, look no further!


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Hi there,
I have about 100 devices on multiple subnets - the network scan has been stalled for an hour now, is this normal or am I experiencing problems?
1 Reply
Dec 14, 2007 at 10:14 UTC
When you click "Settings"->"Network", there is a scanning log at the bottom of the screen. It should give you an update on what it is doing at the moment when you refresh that screen.
Are you sure it is stalled, or is it enumerating services on the 100 pc's it's found? Spiceworks will "discover" devices rather rapidly, then spend a fair amount of time testing the services it finds on each individual device. Check the log on that screen to see precisely what it's up to.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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7/9/2012 - The Current Market Sentiment
The market focusing has been shifted to the US labor report of August which will be released today from the ECB's yesterday awaited decision of buying bonds which came with expected germane criticism too. The single currency rose yesterday after announcing the decision of having the interest rate unchanged at 0.75% but after receiving the decision of releasing unlimited buying bonds plan from the secondary market in an unlimited way during the consequent press conference, the single currency came under the pressure which eased back again brining the single currency again to be traded above 1.26 versus the greenback waiting for today US labor market data which will be closely watched by the markets to have new clues about the US labor market which always takes the care of the Fed which came under a lighter pressure to decide a new QE3 soon following last US non-farm payrolls release of July improved to 163k in July from 64k in June underpinning the greenback.
But anyway now the USD is under pressure as the market sentiment has been shifted from discounting an easing action by the Fed, if the US economy to deteriorate further to such an action from the Fed, if it is not to improve soon since the release of the minutes of the Fed's last meeting in august which can suggest that having non-farm payrolls numbers again below 100k in August as what have been in the previous 3 months to July can warrant a new action by the Fed instead soon of waiting for having negative numbers again as most expectations were referring by these minutes.
God willing, the US labor report of August is expected to show declining of the non-farm payrolls of to 125k with stable unemployment rate at 8.3% as it was in July while the existing cheeriness of having better data are still underpinning the equities markets following this week release of US ADP Employment change of August which rose to 201k from 173k while the consensus was referring to 140k.
By God's will, The single currency is expected to meet resistance again at 1.265 versus the greenback whereas it failed to surpass yesterday and also by the end of last week when it was satisfied by reaching 1.2636 and a continuation of this rising can be met by another resisting level at 1.2693 before 1.2748 which has been reached after the recent parliament elections in Greece in last June while easing down back again can be met with supporting levels at 1.2464, 1.24., 1.2241, 1.2134 before 1.2042 which could stave off the pair falling last month and in the case of falling below it, this can open the way for testing 1.20 psychological level which can be followed by reaching 1.1876 again whereas the pair has rebounded forming its bottom on 7th of June 2010
Kind Regards
FX Market Strategist
Walid Salah El Din
E-Mail: email@example.com


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- Nov 16th - 17th, 2013
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The Dunham Vol. Fire Dept. Gun Show currently does not have dates scheduled. This Belpre, Ohio gun show is held at Dunham Vol. Fire Dept. and hosted by Dunham Vol. Fire Dept.. All federal, state and local firearm ordinances and laws must be obeyed.
Self-Sponsored, all proceeds benefit the Dunham Vol. Fire Department.
60 Tables
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Saturday 7:00am - 9:00am


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Day Two Results (pdf)
GAMBIER, Ohio - The men's swimming team has the advantage heading into Saturday's action, but the lead is anything but safe as they look to win Kenyon's Total Performance Invitational for a third consecutive year. At 848.5 points, the Quakers hold a 96-point lead on Kenyon (752.5) heading into the last day of competition. The other four teams in the field all have less than 300 points apiece.
The night began with the 200 Freestyle Relay. Though Penn didn't get the victory, both its 'A' and 'B' teams placed in the top three. The 'A' team of Rhoads Worster, Alex Porter, Eric Schultz and Kevin Intrieri finished second with a time of 1:21.87.
The Quakers didn't really get things rolling until the middle stages of Friday night's finals. Kyle Yu started it with a win in the 100 Breaststroke, coming home in a time of 56.06. Next up was Worster, who proceeded to set a school record in the 100 Backstroke, breaking Nick Johnson's time from 2011. Worster clinched the victory with a time of 49.60.
Both Worster and Yu would play large roles in the final event of the evening, the 400 Medley Relay. Penn's 'A' team won the event with those two swimming the opening two legs, and Samuel Haley and Porter closing it out in 3:20.38.
Other top finishers for the Red and Blue on the evening included Haley in the 200 Butterfly (second place, 1:50.96), Chris Swanson in the 200 Freestyle (third place, 1:40.52), Brendan Crystal in the 400 IM (fifth place, 4:03.29) and Schultz in the 50 Freestyle (fourth place, 20.80).
Preliminaries resume tomorrow morning from the Kenyon Aquatic Center beginning at 9:30 a.m. The Total Performance Invitational will close out with championship and consolation finals tomorrow night at 5:30 p.m.


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Night in Shanghai
Night in Shanghai
By Nicole Mones
Houghton Mifflin, Hardcover, 9780547516172, 277pp.
Publication Date: March 4, 2014
List Price: $25.00*
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In 1936, classical pianist Thomas Greene is recruited to Shanghai to lead a jazz orchestra of fellow African-American expats. From being flat broke in segregated Baltimore to living in a mansion with servants of his own, he becomes the toast of a city obsessed with music, money, pleasure and power, even as it ignores the rising winds of war.Song Yuhua is refined and educated, and has been bonded since age eighteen to Shanghai's most powerful crime boss in payment for her father's gambling debts. Outwardly submissive, she burns with rage and risks her life spying on her master for the Communist Party.Only when Shanghai is shattered by the Japanese invasion do Song and Thomas find their way to each other. Though their union is forbidden, neither can back down from it in the turbulent years of occupation and resistance that follow. Torn between music and survival, freedom and commitment, love and world war, they are borne on an irresistible riff of melody and improvisation to Night in Shanghai's final, impossible choice.In this stunningly researched novel, Nicole Mones not only tells the forgotten story of black musicians in the Chinese jazz age, but also weaves in a startling true tale of Holocaust heroism little-known in the West.View the Trailer: www.nicolemones.com
Saturday, Mar 8, 2014
Nicole Mones' new novel tells the story of African-American musicians who found respect and appreciation in Shanghai's nightclubs, even as the city descended into Japanese occupation and war. More at NPR.org
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Thursday, Feb 27, 2014
Alan Cheuse reviews Night in Shanghai, by Nicole Mones. More at NPR.org
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East Central Mental Health-Mental Retardation Inc is a Psychologist office located in Tuskegee, AL. A psychologist focuses on the evaluation, prevention, diagnosis, and treatment of mental, emotional and behavioral health issues. A clinical psychologist uses psychotherapy and other counseling skills to improve emotional and mental health.
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The Federal Reserve System (also known as the Federal Reserve, and informally as the Fed) is the central banking system of the United States. It was created on December 23, 1913, with the enactment of the Federal Reserve Act, largely in response to a series of financial panics, particularly a severe panic in 1907.
Over time, the roles and responsibilities of the Federal Reserve System have expanded, and its structure has evolved. Events such as the Great Depression in the 1930s & 9/11 in 2001 were major factors leading to changes in the system.
Adapted from Wikipedia


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After making homemade marshmallows, it's only natural to make S'mores, right? But you know me and my chocolate problem, and when I started thinking about European chocolate, I got an idea…and another idea, and another…well, just look at what I've done…
BRITISH (SCOTTISH) S'MORES…Shortbread, Cadbury's Dairy Milk Chocolate and a marshmallow
FRENCH S'MORES…Mere Poulard biscuits with dark chocolate (preferably French) and a toasted marshmallow
DUTCH S'MORES…Stroopwafels with a very dark chocolate (Dutch chocolate if you can find it) and a toasted marshmallow ~ Version 1
DUTCH SPECULAAS S'MORES…Speculaas Cookies with dark chocolate and a toasted marshmallow ~ Version 2
ITALIAN S'MORES…Latte e Miele Biscotti with Italian (Perugina dark) chocolate and a toasted marshmallow
BELGIAN S'MORES…Biscoff Cookies with Belgian dark chocolate and toasted marshmallow (I was kind of hungry when I was shooting these…can you tell?)
And last, but not least, my favorite ~
BRITISH DIGESTIVE S'MORES…Digestive Biscuits with Cadbury's Dairy Milk Chocolate and a toasted marshmallow! Heaven to a Brit!
I wanted to make Australian S'mores with Anzac Biscuits and Mexican S'mores with Mexican Cookies…well, you get the idea, but there are only so many S'mores a girl can eat in one go! Let me know if you come up with another international version…I'd love to hear what you come up with. In the meantime, I hope you try one of these as they are all deliciously naughty (and very messy)! Enjoy!


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Knowing the Occasion of a Peaceful Death in Intensive Care Units in Thailand
Kongsuwan, Waraporn
Locsin, Rozzano C
Schoenhofer, Savina O
Bibliographic Citation
Nursing & health sciences 2011 Mar; 13(1): 41-6
The purpose of this study was to describe how nurses know the occasion of a peaceful death. The data were generated from individual in-depth interviews with ten nurses who practised in adult intensive care units in the southern region of Thailand. Using a content analysis method, four processes of knowing the occasion of a peaceful death were isolated. They were visual knowing, technological knowing, intuitive knowing, and relational knowing.The clinical implications of these processes provide opportunities for nurses to practise the best end-of-life care during a critical time in a patient's life. This study also strengthens cross-cultural nursing during end-of-life care in high-technology settings, such as the intensive care unit.
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End-of-Life Care in Intensive Care Units -- Save Lives That Can Be Saved, Offer the Dying a Peaceful and Dignified Death Klepstad, P.; Gisvold, S.E. (2003-05)
Brief survey: determining brain death in Canadian intensive care units = Enquête ponctuelle: la détermination de la mort encéphalique dans les units de soins intensifs au Canada Doig, Christopher James; Young, Kimberly; Teitelbaum, Jeannie; Shemie, Sam D. (2006-06)
Dignified Death: Concept Development Involving Nurses and Doctors in Pediatric Intensive Care Units Poles, Kátia; Bousso, Regina Szylit (2011-09)The aim of this study was to develop the concept of the dignified death of children in Brazilian pediatric intensive care units (PICUs). The Hybrid Model for Concept Development was used to develop a conceptual structure ...


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Enjoy dinner drinks and a chance to meet…
Yessi Bello Perez is a reporter at Tech City News, and a regular contributor at tech-related events throughout the UK. Yessi has spent the last year researching and writing about the hottest AI companies in the UK. She will be talking about her key findings in advance of Tech City News' upcoming print magazine, which will include a special report on HealthTech and the use of artificial intelligence within the sector. Whilst her knowledge of AI spans across various different verticals within tech, Yessi is particularly interested in the way in which innovative HealthTech companies are leveraging the technology to diagnose disease.
This dinner is perfect for…
HealthTech, MedTech & AI Founders, CEOs, entrepreneurs.
The dinner conversation will be…
Yessi will be talking about:
✓ The key new startups using AI to shake up the UK's healthcare industry.
✓ What tech giants such as Google and Microsoft are doing with AI and healthcare.
✓ What is attracting funding in this space.
✓ An overview of AI and HealthTech trends in 2016 and prediction of key trends for 2017.
Expect to hear what's cutting edge in the sector and to leave with some great take-aways for your own strategy.
Who's Yessi Bello Perez?
Yessi Bello Perez is a reporter at Tech City News, the UK's leading B2B tech title. Prior to joining Tech City News in January 2016, Yessi spent a year reporting on the global bitcoin and blockchain scene for one of the industry's leading publications. With a background in lifestyle and multimedia journalism, her work has been published in Cosmopolitan, HELLO! online and Time Out.


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NEW YORK (TheStreet) -- The selloff in new tech momentum stocks such as Netflix (NFLX)Facebook (FB) and Twitter (TWTR) presents a buying opportunity for savvy investors, said fund managers who are predicting a rebound in their prices.
Yet others warned against catching a falling knife, as a lack of market momentum this year sees investors shun growth stocks in favor of their cheaper value peers.
The market is drawing sharp differentiation between old and new tech stocks, with Microsoft (MFST), Cisco (CSCO) and IBM (IBM) -- companies with solid balance sheets and well-established business models -- all higher for the year to date. Several analysts have re-labeled them value stocks, despite technology traditionally being viewed as a growth sector.
By contrast, Twitter, Facebook and Netflix are seen as trendy new-tech stocks, where traditional financial ratios mean less, as valuations are predicated on forecasting future social trends. These companies rallied hard -- especially in the second half of last year -- amid a backdrop in which momentum and optimism drove prices higher.
But as the rally enters its sixth year and concerns rise around correction risks, investors have re-orientated away from trendy names.
Warren Financial Service Chief Investment Officer Randy Warren views the selloff in new-tech stocks as a buying opportunity for investors who were unable to buy-in during their IPOs.
"You have to know what you're buying, as people who have too much in these stocks are hitting the panic button. But if potential investors sit on their hands, these stocks could come roaring back," he said in a phone interview.


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Hi I plan on doing a water cooled build for my next rig I gonna use a NZXT phantom 820 and a 1000w corsair PSU and 32Gs of Dominator® Platinum and two cards of ether the new AMD volcanic islands or geforce nvidia maxswell but I want to know what size fittings and tubing and if a Aquacomputer Aqualis 450ml Reservoir/pump is a good choice for the case and what size rads I need. Help would be most welcomed and thanked.
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Perth Ideally Poised To Become Australia's Next Big City
An in-depth look at Australia's second fastest-growing city.
"Ask Perth residents about their city and they will rhapsodise about the pristine beaches of Cottesloe and Scarborough, the Margaret River wineries and the charm of Rottnest Island. The promise of paradise on a penny-pinching budget is a potent lure for east coast Australians."
Thanks to Brenda Meyer