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The Grand Auctioneer was the owner of the Lifemarket on Bryce's Asteroid.
As a young man, he majored in genetics and horticulture on the planet Bryce. One day, he learned how to reanimate dead cells. He was exiled from Bryce and opened the Lifemarket on Bryce's Asteroid.
When the Sixth Doctor and Peri visited Bryce's Asteroid, he took a liking to Peri. He thought the Doctor was a reanimated, but malfunctioning, dead body and had him sent to the workshop to be fixed.
When the Doctor ruined his business, Peri suggested he go back to topiary, as he was very good at it. (PROSE: A Star is Reborn)


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Tenant Held in Killing: Pressure and Tragedy
By JAMES C. McKINLEY Jr.
Published: May 16, 1991
Mehmet Lijkovic was under a lot of pressure.
He had been working seven days a week as a building porter on the Upper East Side of Manhattan to make up some of the money he lost in the strike that had left him without a paycheck for two weeks.
At his home in Queens, neighbors were complaining that his children were too noisy. The condominium board was threatening legal action.
On Tuesday night, the police said, Mr. Lijkovic finally broke. He went to the apartment of the board member he thought was behind the complaints about his children, the police said, and shot the 40-year-old furrier dead in front of the man's wife.
The police gave the following account of how Mr. Lijkovic, 40, ended up charged with murder:
When Mr. Lijkovic arrived home at Village Park Gardens at 64-17 72d Street in the middle-class neighborhood of Middle Village shortly before 7:30 P.M., he found a letter from the board that said he would "be held liable" for the noise being caused by his two children. Some residents also complained that the children had been unruly in the building and about graffiti linked to them. Shot 8 Times
Mr. Lijkovic stormed upstairs to confront Warner Vanderhooft, the board member he thought was behind the letter. He took a 9-millimeter pistol with him. At first the two men began shoving each other in the hallway, each accusing the other of making trouble, said Sgt. George Zaroogian of the 104th Precinct detective squad.
Mr. Lijkovic pulled the gun from his waistband, and, in a fury, shot the taller, stronger man eight times, killing him instantly, according to the police.
Yesterday, neighbors in the five-year-old three-story apartment building and along the quiet residential street were trying to fathom how such a killing could have happened.
Mr. Vanderhooft, who owned TNW Furs, at 307 Seventh Avenue, near 27th Street, in Manhattan, lived with his wife, Margaret Bussfeld, in Apartment 3A. The police said he had apparently complained several times in the past about noise the children were making.
Mr. Lijkovic, who was raised in Yugoslavia, came to America about 20 years and worked for a long time as cook at a restaurant in New Jersey before taking a job as a porter seven years ago, friends and relatives said.
Five years ago, he and his wife, Serveta, bought a two-bedroom apartment, one of 12 apartments in Village Park Gardens, for $170,000 when it was under construction. The couple have a daughter, 12, and a son, 11.
Mr. Lijkovic was described by relatives and friends as a quiet, gentle person whom they would not expect to get into a fight. One relative, who did not want to be identified, said: "He was a good person staying out of trouble. I have known him for over 17 years and I was shocked when I came home from work and heard he had done this." She added, "It's unbelievable."
Kola Cacaj, the superintendent of the apartment building at 340 East 74th Street in Manhattan, where Mr. Lijkovic was a porter for seven years, also described him as someone who kept to himself and rarely spoke. But Mr. Cacaj observed, "He had a hot temper."
Mr. Cacaj said the suspect had been working seven days a week over the last two weeks since the building workers' strike ended. He described the porter as "very nervous from working too much" and angry that the building's owner had not hired another porter to help him. As a result, Mr. Lijkovic was threatening to quit, he said.
Mr. Cacaj said that Mr. Lijkovic claimed to have a pistol permit and was very proud of it and that he often went to shoot at a pistol range. 'To Pay Him Back'
Mr. Cacaj, who, like Mr. Lijkovic, is of Albanian descent, said that in Albania, "soon as a boy hits 14, you buy him a gun and tell him to protect himself -- when someone attacks you or hits you, you're supposed to pay him back."
The police were unable to determine yesterday whether the handgun used in the killing was licensed. Mr. Lijkovic was arrested outside the building shortly after the shooting with the pistol in his hand.
Some residents of the building said Mr. Lijkovic's two children had repeatedly caused problems, writing on people's doors, ringing doorbells and allowing other children into the building.
But other residents said the children's behavior was not out of the ordinary for children of that age.
Anthony Sorisi, who moved out of the building in February after living there for three years, said: "It was just kid stuff. The kids were just playing. Every once in a while they'd let other kids into the building. Nothing to shoot somebody over, you know what I mean?"

subset: commoncrawl


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Reading & Research
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For more information on area tourism, visit: Southern Delaware Tourism


text:
Iryna Kysil
Kyiv - Ukraine
No approved endorsements
About Iryna Kysil
Marketing and PR specialist with special focus on IT and commercial property markets.
Education: Kyiv National Taras Shevchenko University graduate, have lived and studied in Ca, USA. Competence in sociology and research (MS obtained), Marketing.
If you need more detailed information on my professional background, please, refer to my profile at linkedin.com (find the direct link below).
If you notice offensive or incorrect information, please contact us.


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Unlike pages on the visible Web (that is, the Web that you can access from search engines and directories), information in the Deep Web is just not visible to the software spiders and crawlers that create search engine indexes. Since this information makes up the vast majority of available content on the Web, we are potentially missing out on some pretty amazing resources.
Via Karen Bonanno


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Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.


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ESO EHR + Philips MRx monitor ?'s
Discussion in 'EMS Talk' started by Kevinf, Dec 27, 2016.
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No approved endorsements
About Shanna B. Groban
LuxPro Staffing was created with a passion for excellence in mind. Founded on the basic principle of providing the highest quality service and response time to our clients and candidates without compromising exactly the right match. Our main goal is to provide you with a luxury recruiting experience that respects the unique demands of your organization as well as the unique skills and goals of every candidate.
LuxPro Staffing provides professionals to corporations and law firms on a contract and direct-hire basis.
General Office Support, Accounting and Finance, Marketing, Legal, Human Resources
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text:
Sakura (Cherry Blossoms)
To play the media you will need to update yourFlash plugin.
Handbells (Handbell Acc)
3 Octaves of Handbells Level 2. Arranged by Kiyo Watanabe. Shawnee Press. 8 pages. Shawnee Press #HP5432. Published by Shawnee Press (HL.35018805).
Item Number: HL.35018805
9x12 inches.
Kiyo Watanabe has masterfully arranged this traditional Japanese melody for 3 octaves of handbells. The plaintive melody is supported by both chordal and undulating eighth accompaniment. This piece is a good challenge to work on sensitivity in dynamics and evoking a mental picture of lovely cherry blossoms. 44 measures.


text: Pages in category "Series 23" The following 83 pages are in this category, out of 83 total. . Episode:…And Nothing But The Truth4 Episode:Trouble on the Aldbourne- Part 2 Episode:The Last Stop Part 1A Episode:A Life of Lies Episode:Assault On Sun Hill Part 2 Episode:Assault On Sun Hill Part 1B Episode:Baby Trade Episode:Back From The Brink Episode:Back From The Dead Episode:Behind Closed Doors (2007) Episode:Better Off Dead (2007) Episode:Blackmailed Episode:Blood Money Episode:BrotherhoodC Episode:Caught By The Killer Episode:Chain of Events Episode:Code of Silence Episode:Collateral Damage (2007) Episode:Cop Killer, Part 1 Episode:Cop Killer, Part 2 Episode:Copy Cat Killer Episode:Cracking Under Pressure Episode:Crash TestD Episode:Daddy's Girl Episode:Day of Reckoning (2007) Episode:Dead and Buried Episode:Dead Men Don't Tell Lies Episode:Deadly Intent D cont. Episode:Deadly Secrets Episode:Deadly Shame Part 1 Episode:Deadly Shame Part 2 Episode:Death or Glory Episode:Diamonds Are Deadly Episode:Dicing With Danger Episode:Do or DieE Episode:Exit WoundG Episode:Games of War Part 1 of 3 Episode:Games of War Part 2 of 3 Episode:Games of War Part 3 of 3 Episode:Getting Away With Murder Episode:Good Cop, Bad CopH Episode:Hunter On The StreetI Episode:Identity Fraud Episode:Inner DemonsJ Episode:Judgement Call (2007)K Episode:Killer On The Run Episode:Killing Me SoftlyL Episode:Lies That Kill Episode:Located Part 1 Episode:Love, Lies and LimosM Episode:Man Down Episode:Match Day Violence Episode:Mistaken Identity Episode:Model Murder Uncovered Episode:Moving Target Part 1 Episode:Moving Target Part 2 M cont. Episode:Moving Target Part 3O Episode:Old Wounds (2007) Episode:On The Edge Episode:Operation Brass BallsP Episode:Pride Before A FallR Episode:Reluctance Episode:Role Model Episode:Rough Justice Episode:Russian RouletteS Episode:Stealth Attack Episode:Stimulation Episode:Sweet RevengeT Episode:Take It To The Grave Episode:The Last Stop Part 2 Episode:Tortured Soul Episode:Trail of Blood Episode:Trapped Episode:Trigger Happy Episode:Trouble on the Aldbourne Part 1U Episode:Uncut Killer Episode:Up In SmokeZ Episode:Zain- Fake Information Part 2 Episode:Zain- No Going Back Part 1 Episode:Zain- Panic Episode:Zain- The Greatest Day Episode:Zain- The Net Closes Part 4 Episode:Zain- Uncovered Retrieved from "http://thebill.wikia.com/wiki/Category:Series_23?oldid=4798" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: The Bill: episodes Add category Cancel Save


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The most read articles Timpul.md
Newest articles Timpul.md
- 17 Februarie 2017, ora: 08:10 ROMANIA is proTESTING. The unseen story behind the largest protests over the past 25 years
- 09 Septembrie 2016, ora: 11:09 Interview with Prime Minister Pavel Filip: "We will steer clear of partisan politics and will govern with one single goal in mind: results"
- 30 August 2016, ora: 09:56 To the Secretary of State or the United States of America, His Excellency, Mr. John Kerry
- 25 August 2016, ora: 14:06 In next 10 years we can do more than in past 25 years. IPN Series
- 20 Iunie 2016, ora: 14:08 Turkey LGBT: Scuffles at banned Istanbul transgender event


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Alumni Email
Your Duke address
Time changes everything – except your @alumni.duke.edu email address. This free email account lets you stay in touch with friends no matter whether you change jobs or email providers. Use it as a full account or forward to your preferred inbox – it's your permanent link to Duke.
Get It
You're two quick steps away from having your @alumni.duke.edu email.
First, set up a OneLink login. (You may have already done this to access the alumni network or other Duke sites.) This will establish that you're eligible for the email service and provide access to your account.
Next, complete the form provided by the Duke Office of Information Technology. Be sure to set a forwarding address if you don't intend to check your inbox regularly.
Use It
If you're using your @alumni.duke.edu email as a forwarding address, you'll receive emails in your preferred inbox. However, you won't be able to reply or send messages from your @alumni.duke.edu address.
For more information about accessing full email services, call the OIT Help Desk at (919) 684-2200.


text:
Documentation and Configuration Manager
Documentation and Configuration Manager The Client: High Value, Low Volume electromechanical manufacturing The Role: The Documentation and Configuration Manager plans, coordinates and manages programs and processes designed to ensure effective management and implementation of the company documentation and configuration management processes.A proven track record of success in the Aerospace, Medical Device, Automotive or similar, regulated industries is a must.This position is responsible for managing and executing the global quality strategy for documentation and product configuration management, and overseeing configuration management for government programs. Responsibilities include managing the planning and implementation of the company Product Lifecycle Management (PLM) system (Agile); development and implementation of related corporate processes, process maps, procedures and tools; and oversight of the global document control and configuration management activities. This position will also be responsible for leading the global implementation and integration of Agile with the company Enterprise Resource Planning (ERP) and other operational systems, ensuring the integrity of data between Agile and the ERP system, and establishing the process for maintaining an effective change management process. Key Responsibilities: Identify and leverage best practices and processes in support of the Quality Management System (QMS). Monitor new industry and regulatory agency trends such as emerging standards and regulatory requirements, and new technology opportunities relative to documentation and configuration management. Oversee Configuration Control Boards (CCB) and Functional/Physical Configuration Audits (FCA/PCA). Participate in NPI and sustaining engineering (design change, process change, etc.) projects and other company initiatives to ensure they adhere to and satisfy QMS requirements. Ensure configuration management requirements are met for all government and commercial programs. Support production and release of internally and externally manufactured products and components. Gain cross-business consensus, where possible, to establish new processes and implement effective change management. Ensure the documentation and configuration management process is compliant with all applicable industry, local, national and international regulatory requirements and standards. Continuously seek innovative ways to improve business productivity through the introduction of new documentation system technologies, methods and controls. Deploy good documentation control and configuration management practices. Select, implement, train users and manage corporate documentation and configuration management tools. Develop and effectively manage operating, staffing, and capital projects budgets for area(s) of responsibility. Ensure that documentation management professionals are fully competent for their roles, that they are effectively trained, organized and deployed to deliver high quality service to users, meet time commitments and receive appropriate training and mentoring. Salary: Circa £40,000 plus benefits This job was originally posted as www.totaljobs.com/job/66874377


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CIOs in Hong Kong stepping up fight against lax BYOD practices, new data reveals
One in three CIOs polled by Robert Half say a 'lack of employee knowledge and skills around data security' will be the biggest security risk their organisation faces in the coming five years.
The study, which surveyed 100 CIOs and CTOs in Hong Kong, found that almost three quarters (74%) of respondents allow their employees to access corporate data on their personal devices.
Only one respondent said their company was doing nothing to protect the company's data on BYOD, while deployment of...


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If an item you ordered from TextbookRush does not meet your expectations due to an error on our part, simply fill out a return request and then return it by mail within 30 days of ordering it for a full refund of item cost.
Learn more about our returns policy
Description: More than a Hobby takes readers inside the mind of a low-key, likable Oklahoma entrepreneur who has created a unique shopping experience. How did his company go from a $600 loan to $1.3 billion in annual sales in just thirty-one years-especially when he wasn't groomed for this work either by heritage or by education?Green was willing to go against the tide, allowing faith to play a huge part in the business. More Than a Hobby describes how this wildly successful business was built not on business-school theory, but on the founder's own experience as a grassroots store manager.
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List price: $14.99Copyright year: 2010Publisher: Thomas Nelson IncorporatedPublication date: 7/26/2010Binding: PaperbackPages: 224Size: 5.00" wide x 8.00" long x 0.63" tall Weight: 0.462Language: English
David Green is a clinical psychologist who has worked therapeutically for more than 30 years with young people and their families. From 1988 to 2010 he was Clinical Director of the Doctor of Clinical Psychology programme at the University of Leeds. He has been particularly interested in the role clinical supervision plays in the education of healthcare professionals.

Gary Latchford is a clinical psychologist working in physical health, based at the department of Clinical and Health Psychology at St James's Hospital in Leeds. Since 1996 he has also been research director for the Doctor of Clinical Psychology programme at the University of Leeds. His clinical and research interests are mostly around psychological interventions in medical settings, and he has published several papers and book chapters on this topic.

Dean Merrill, former publishing executive with Focus on the Family and International Bible Society, is author or coauthor of 31 books, including The God Who Won't Let Go and Sinners in the Hands of an Angry Church. He and his wife live in Colorado Springs, Colorado.
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Membership is one of the best ways to become involved in the Old Red community. Members receive early word about Old Red programs, exhibits and classes. We offer both Museum Memberships for individuals, couples, and families as well as Corporate Memberships at several different levels. Click on Membership Levels for individual, couple or family memberships or Corporate Membership for more information. Or, email email@example.com or call 214-757-1907 for general information.
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There are many ways to participate in the exciting programs and activities at the Old Red Museum. From volunteering and internship opportunities to donations and sponsorships, you can choose to be an active member in the history of your community.
Stay up to date with the latest at the Old Red Museum by subscribing to our newsletter. Also, find out what we've accomplished in the past by reading our previous newsletters.
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Archives of Cultural Exchange (Dr. Carl L. Johannessen)
Expand Messages
- Thank you for your submission, Terry.Cal, Larry, and some of you might be interested in the efforts and following proposal by Carl (University of Oregon-Eugene). Terry, Rick, myself, and others here are familiar with Dr. Johannessen's presentations at the Atlantic Conference-Halifax, Nova Scotia archives and 2009 online conference.
The "Archives of Cultural Exchange" is a 501(c)3 non-profit Educational Organization founded by Professor Emeritus Carl L. Johannessen as a way of preserving the unpublished records and manuscripts of retired professors and other researchers. It is his contention that a lot of research data is lost, especially in leading edge research, because the pioneers are speaking too far ahead of their time. It was and is his hope that making this data openly available will stimulate exploration across disciplines and types of science, furthering human understanding.
Professor Johannessen, along with a small team of dedicated staff and researchers, hopes to open up research to the everyman. This is something more akin to the idea of the Renaissance Man as envisioned by Leonardo da Vinci and other researchers of the Renaissance and Age of Reason, in the days before specialization and sub-specialization removed the impetus for cross-discipline collaboration. It is our sincere hope that this project reminds scientists and researchers of this valuable area of and tool for research.http://www.archivesofculturalexchanges.org/index.htmlProfessor Emeritus Carl L. Johannessen: carlj@... or maizeresearcher@...
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Nimbleness is a quality that can be applied to armor, allowing its wearer to have greater freedom of movement than would normally be available for the standard armor type being worn.[1]
|This article is a stub. You can help the Forgotten Realms Wiki by expanding it.
References Edit
- ↑ Sean K. Reynolds, Duane Maxwell, Angel McCoy (August 2001). Magic of Faerûn. (Wizards of the Coast), p. 137. ISBN 0-7869-1964-7.


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Conestoga Collection
This nice and practical Shaker Jewelry Armoire handcrafted by the Amish has 4 legs, 6 drawers and 2 necklace doors for your convenience. Please call to speak to one of our Amish furniture specialists with questions or ordering assistance. This Shaker Armoire is a perfect gift item!
© Copyrights 2003 - 2017 DutchCrafters All rights reserved.


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Description: A form of Thai Yoga Massage that focuses on the three Ayurvedic body types? Explains how to determine a person's Ayurvedic body type and provides hands-on techniques for working with them? Brings the practices presented in Thai Yoga Massage to a new customized level of therapeutic healing The traditional healing arts of Ayurveda and Thai Yoga Massage have a deep and integrated relationship that provides an unparalleled modality for restoring body, mind, and spirit. Although it originated in India, over the centuries Ayurveda has been assimilated into the predominant Thai culture and has evolved into a distinctive folk medicine. With the growing popularity of Ayurveda and Thai Yoga Massage, there is a renewed interest in reuniting these practices into a powerful therapeutic alliance. "Thai Yoga Therapy for Your Body Type" bridges the practice of Thai Yoga Massage with its ancient Ayurvedic roots to offer a complete and holistic healing modality. The authors first explain in detail the fundamental principles of Ayurveda and then recommend daily practices for each of the three main body types of vata, pitta, and kapha. Practitioners learn how to customize their work with the appropriate massage approach, recommended yoga asanas, breathing techniques, and diet and lifestyle tips. More than 50 illustrated, full-body Thai Yoga Massage postures are presented as well as a massage flow for each body type. The authors indicate the Ayurvedic benefits of each posture and detail any precautions that should be followed in this dynamic practice of transformative healing.
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List price: $24.95Copyright year: 2006Publisher: Inner Traditions International, LimitedPublication date: 6/15/2006Binding: PaperbackPages: 240Size: 8.50" wide x 11.00" long x 0.75" tall Weight: 1.430Language: English
One of the first Thai yoga massage practitioners in North America, Kam Thye Chow has taught Thai massage in Thailand and throughout Europe, Canada, and the United States. The author of Thai Yoga Massage and Thai Yoga Therapy for Your Body Type, he is the founder and director of the Lotus Palm School in Montreal. Kam Thye Chow lives in Montreal.
Emily Moody is an Ayurvedic educator, yoga teacher, and teacher for the Lotus Palm School and has worked closely with Kam Thye Chow since 1999.
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text: > > No, this is skipped. You can select sets of packages, but "choose > > indivual packages" is gone. :-( I don't think this is a progress. > > Well, you have the option of selecting a group, such as KDE, Gnome, web > server, email server, etc.. then you are able to select certain > individual packages from those. You just can't select each individual > package that exist like the old days. Some are automatic when you > select/deselect within that group. > I'd have to cast my vote for having "choose individual packages" back. There are many times I want a package out of a group, but not the "Default" packages in that group. In many cases, at least with RH9, the "default" packages are not even dependancies for a sub-package in the same group. (At least for the packages that I needed at the time. Can I remember what they were? No, of course not. ;) ) Thanks, Adam Debus Linux Certified Professional, Linux Certified Administrator #447641 Network Engineer, ReachONE Internet adam reachone com


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Report summaryOil/NGL production capacity in Nigeria is expected to remain relatively flat in the future, although output will continue to be at risk from theft. Capacity from onshore and shallow water Niger Delta fields continues to be hampered, but the situation has improved since 2009.
What's included?
This report includes 1 file(s)
- Nigeria oil supply summary PDF - 1.72 MB 14 Pages, 4 Tables, 6 Figures


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Chief Keef - Spread Da Word (Official Video) MP3
Official Music Video 2012. My Page: http://www.facebook.com/ProductionHipHopVEVO.
Chief Keef | Spread Da Word (Instrumental) | [Prod. by @XyzRapper] MP3
Originally produced by Young Chop Download Link: http://www2.zshares.net/78xbcz0n972f.
Chief Keef - Spread Da Word (Official Video) 2014 MP3
Chief Keef-Spread The Word Chopped & Screwed MP3
3rd chopped song.
Chief Keef - Spread The Word MP3
Spread Da Word - Chief Keef MP3
Chief Keef - Spread Da Word: Zion Hill Dance Squad MP3
Dancing to :Spread Da Word-Chief Keef.
Chief Keef - Spread Da Word MP3
Chief Keef- Spread The Word (Chopped and Screwed) [HQ] MP3
Chief Keef Spread Da Word Road To Bang Pt 2 Mixtape 01 20 2013 MP3
Chief Keef "Earned It" Music Video prod by @twincityceo Directed by @NICKBRAZINSKY x @EICKHOFKALE MP3
Chief Keef - Spread The Word. prod by. Young Chop MP3
3 UnoFuego Chief Keef Spread Da Word Remix MP3
Chief Keef - Kobe Freestyle (Shine) MP3
Written Freestyle To Famous Rapper Chief Keef's "Kobe" Song .. Check It Out LISTEN , COMMENT , LIKE , SUBSCRIBE & SPREAD THE WORD !!!!!! Download ...
Chief Keef - I Don't Like Remix (feat. Childish Gambino, Meek Mill, Drake, and Big Sean) MP3
Download For FREE here!! http://snd.sc/NX7iwn NEW JaHa MIX!!!!! CHECK IT OUT SPREAD THE WORD!!! THANK YOU SOO MUCH FOR LISTENING IT ...
Chief Keef - W.W.Y.D. [Instrumental] (Prod. By The Brain) + DOWNLOAD LINK MP3
DOWNLOAD LINK: http://www.hipstrumentals.com/2015/02/chief-keef-w-w-y-d-instrumental-prod-by-the-brain/ For ORIGINAL INSTRUMENTALS or to UPLOAD ...


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Separate names with a comma.
Discussion in 'Original Pictures Forum' started by TXNSLighting, Feb 17, 2013.
Lol that's no joke!
Posted via Mobile Device
Hahahahaha no lie there!
installing a new lawn at this house next week!
Is this an installation on a site without irrigation? Funny how people let things go then suddenly go for it.
No, irrigation... So no warranty from me! Hope he keeps on his word to water...
Well I wouldn't lay the sod until it comes in green....we no longer do yards without irrigation.
He will be good at watering. Hes retired and home all day. Hes ready for a nice good lawn.
it looks like in the first couple of pics the mulch is 4" below the brick line which is the recommended amount for residential in our area, the wheep holes are still 4" above grade. Lettering looks good
Why thank you sir. I love it. Yes sir, first job i ever did i covered up the bricks and heard about it from the homeowner. Have always made note of that little detail from then on.
Well guys, looks like my assault on lawn maintenance is coming to an end. I have the business up for sale. Going to focus more on my haul business, lighitng, acreage mowing and dirt work. Going to invest in a bobcat with mower attachment. We shall see how it goes!


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Chiller TV Online
Now here's a great way to kick off your weekend! A little snack if you will! Chiller TV's upcoming zombie themed show "Remains" is on its way, and we have the first two stills right here for you to digest. Toothpick not included.
Look for the show, which is based on the graphic novel by Steve Niles and Kieron Dwyer that tells the story of a blackjack dealer and a dancer in Reno, Nevada, who find themselves trapped in a casino when a nuclear accident zombifies everyone on earth, to premiere on Friday, December 16th, at 10:00 PM.
Grant Bowler ("True Blood"), Lance Reddick ("Lost"), Tawny Cypress ("Heroes"), and Miko Hughes (Pet Sematary) all star in this latest adaptation directed by Colin Theys.
More as it comes.
Got news? Click here to submit it!
See what remains in the comments section below!


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BERKELEY – Imagine two central banks. One is hyperactive, responding aggressively to events. While it certainly cannot be accused of ignoring current developments, its policies are widely criticized as storing up problems for the future.
The other central bank is unflappable. It remains calm in the face of events, seeking at all cost to avoid doing anything that might be construed as encouraging excessive risk-taking or creating even a whiff of inflation.
What I have just described is no mere hypothetical, of course. It is, in fact, a capsule depiction of the United States Federal Reserve and the European Central Bank.
One popular explanation for the two banks' different approaches is that they stem from their societies' respective historical experiences. The banks' institutional personalities reflect the role of collective memory in shaping how officials conceptualize the problems that they face.


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UPDATE: Tiger and Elin Working on Marriage
Tiger Woods' wife Elin Nordegren visited her golf star husband in the Mississippi rehabilitation center where he is being treated for sex addiction, People.com reported. She may be seeking to reconcile. Tiger Woods was cited at the Pine Grove Behavioral Health and Addiction Services clinic in Hattiesburg, Mississippi. Read why ESSENCE Relationships Editor Demetria L. Lucas finds Tiger Woods' Vanity Fair cover "Thuggish."
Read More
Latest News


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New, Revenge Scoop From Christa B. Allen!10/19/2012
Revenge: Scoop on the future of Emily's plan for revenge from E! Online's Kristin Dos Santos
Here's some scoop on Revenge from Kristin Dos Santos' Spoiler Chat Daily for Friday 10/19/12.
Katie C.: Wanting Revenge scoop with a vengeance, please!!!
Christa B. Allen tells us that Emily's plan is about to go global! "I can tease that Emily [will be] getting involved with people from around the world," she says. "You see people getting involved with her revenge plot that you never would have expected. Lots of new people coming onto the scene." New faces means new drama, and we love it!
Revenge returns with episode 2×04 'Intuition,' on Sunday, October 21, 2012 at 9pm on ABC. Revenge stars Madeleine Stowe, Emily VanCamp and Gabriel Mann.
In Revenge 2×04 'Intuition,' playing with the Graysons is definitely like playing with fire, and Amanda learns that the hard way. Meanwhile Emily and Aiden each get blindsided, and the wheels start turning as Daniel begins to take matters into his own hands.
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the inconsiderate and loud guests staying there. The building is old and has wooden floors upstairs as well as thin walls. Any time one of these certain guests walked by, it was like an elephant stomping down the hall. Totally of their own doing, its not difficult not to slam your heel down when you walk. If you stay here, please remember that.
Staff were nice, bathroom ok, pool table should just be free, breakfast was a miss.
Maybe the downstairs rooms are better, but if you are upstairs and a light sleeper, you are at the mercy of other guests.
- Official Description (provided by the hotel):
- Located in Georgetown, Guest Inn Muntri provides both dormitory and private air-conditioned rooms with free Wi-Fi. It . Free parking and a 24-hour front desk are available. In the common area, guests can play billiards, watch TV and get drinking water from the water dispenser.Simply furnished, all rooms come with a desk. Bathroom facilities are shared, and are equipped with cold and hot-water showers. There is a shared computer in the common area which guests can use.Guest Inn Muntri is a 10-minute walk from KOMTAR, 1st Avenue and Prangin Mall. It is a 15-minute walk from Penang Jetty and a 30-minute walk from Gurney Drive. Penang International Airport is a 30-minute drive away.Bicycle rentals and day trips can be arranged at the tour desk. The inn also provides luggage storage and laundry services.Guest Inn Cafe serves Western dishes, snacks and desserts. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Orbitz, Hotels.com, Travelocity, Ctrip TA, Priceline, Booking.com, TripOnline SA, Hotwire and Cheap Tickets so you can book your Guest Inn Muntri reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Guest Inn Muntri Penang/George Town


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"A friend of mine, who is an actress, told me that when the casting for her recent movie came down to two actresses, the casting director chose the actress with more Twitter followers. I see this becoming a trend in the music industry. For me, this dates back to 2005 when I walked into my first record-label meetings, explaining to them that I had been communicating directly with my fans on this new site called Myspace. In the future, artists will get record deals because they have fans—not the other way around.
Another theme I see fading into the gray is genre distinction. These days, nothing great you hear on the radio seems to come from just one musical influence. The wild, unpredictable fun in making music today is that anything goes. Pop sounds like hip hop; country sounds like rock; rock sounds like soul; and folk sounds like country—and to me, that's incredible progress. I want to make music that reflects all of my influences, and I think that in the coming decades the idea of genres will become less of a career-defining path and more of an organizational tool.
This moment in music is so exciting because the creative avenues an artist can explore are limitless. In this moment in music, stepping out of your comfort zone is rewarded, and sonic evolution is not only accepted…it is celebrated. The only real risk is being too afraid to take a risk at all."


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When I was asked to write a column on ITIL's life story in honor of Happy ITIL® Day 2016, I didn't have to think long. ITIL is simply too important to let this pass. And as millions have read the books I wrote on ITIL, it was also something I couldn't refuse.
But what message would be interesting for the people looking into Happy ITIL Day? The answer, I thought, should be in the very beginning of ITIL's lifecycle: the last letter of its original name – GITIMM.
Thirty years ago, in 1986, CCTA's Roy Dibble changed that original name into ITIL. Why? because of that last letter, M. It simply wasn't a method...
So if it was not a method, then what was it, Dibble asked?
Read the entire column at Van Haren Publishing and you'll know :-)


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Scotland to feel the pinch as swarms of midges descend
Scotland is about to come under attack from swarms of bloodthirsty predators as forecasters predict that midges will descend in vast numbers this week amid "perfect" conditions of heavy rain and sunny spells.
Any hopes that the cold winter might have killed the midge larvae have been dashed.
"The larvae are well protected underground over the winter and may even have got extra protection from the thick snow," said Alison Blackwell, who runs the Scottish Midge Forecast website.
The insect may be more resilient in cold weather than its natural predators, bats and birds.
"Warm weather this weekend will result in a big increase in midge numbers next week and if it remains wet one day and sunny the next that can give high numbers…


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Am I abnormal? Maybe. Am I perverted? Probably. Am I a sissy? Definitely! This blog is about those things that make a sissy swoon... at least this sissy. Topics that interest me include chastity, enemas, fisting, cock sucking, sounds, tampons, condom sucking, makeup, analingus, forced feminization, debutantes, ball gags, douches, corsets, bimboism, prostate milking, orgasm denial, fashion, erotic hypnosis, sexual humiliation, petticoats, panties, transgender fiction and a whole bunch more!
Saturday, July 21, 2012
3 comments:
Dear Sarah,ReplyDelete
Love your blog dear. You should check mine out. Leeanne's Sissy Musings.
Great photos, awesome spec's :)ReplyDelete
love your site!
- AnonymousJuly 26, 2012 at 9:13 PM
i wouldn't wish to speculate on the spectacular spectacle of the speculums! but they do have a myriad possibilities of potential for very exciting uses in sexual play, don't they? :) (michael g.b.)ReplyDelete


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Student life in Australia
Studying at ACIT & IFTV is more than just being at College – it is a life rewarding experience!
Australia is a continent with an excellent climate, boundless opportunities, and fantastic quality of life, which is the perfect place to study, work and live.
The college campus is situated on the Gold Coast, Australia's fastest growing city and most popular holiday destination.
About the Gold Coast
The Gold Coast is very popular with international visitors and offers endless activities to choose from.Relax over coffee at a beach side café, shop in boutique designer stores, or take in the thrills of our many theme parks.
Our world famous white sand beaches boasts some of the best surfing in Australia, and the Hinterland Mountains and rain forests are literally minutes away.Bush walking, biking and other adventure sports are all the rage in the safe, clean environment of Queensland.
The Gold Coast is also home to numerous internationally recognised golf courses, sporting facilities, parks and the magnificent Broad water – a blue water paradise for safe boating and recreation.
Gold Coast Campus
The ACIT & IFTV campus is located at Varsity Lakes, the Gold Coast's biggest IT hub in a scenic lakeside niche.
Featuring state of the art facilities that offer a unique combination of training and recreation, ACIT is well positioned amongst education industry leaders.
Classrooms are equipped with fast networked computer sand licensed software applications.Virtual environments and lab facilities are provided for practical exercises and hands on training, and every student has broadband internet access for all course related activities.
Library facilities are generally not required but arein close proximity to the campus.
Address: Level 2, 246 Varsity Parade, Varsity Lakes, Queensland 4227
Phone: +61 7 5578 8122 Fax: +61 7 5578 8007 Email: firstname.lastname@example.com
South East Queensland is ideal for holidaying and studying. The local climate is sub-tropical with temperatures varying between 17°C and 28°C during the day, and water temperatures vary from 19°C to 21°C all year round – perfectly suited to outdoor activities such as surfing and golf.
Find more information on Queensland weather at the[Bureau of Meteorology]
Culture & Living
The optimistic Aussie way of life creates an environment where your dreams can come true, and with over 200 languages spoken in the sub-continent, Australia offers a true multicultural experience.
The majority of Australians are ecologically sensitive and concerned about preservation.
Weekend beach trips are very popular, and Aussie Beach Culture, is similar to that of the Mediterranean, South America and California.To view live images and surf reports from beaches in Queensland and other states, visit[Coastalwatch].
The Gold Coast is a renowned tourist destination and presents visitors with a multitude of sporting facilities such as golf courses, large sport fields, tennis courts and a variety of water sport activities.
The Gold Coast also has a number of world renowned theme parks such as [Seaworld], [Movie World], [Wet 'n Wild Water World],[Dreamworld], as well as a large number of environmental parks such as the [Currumbin Bird Sanctuary]and [Fleay's Fauna Park]to showcase Australian wildlife – all within a 30 minute drive from the College.
Dining and Entertainment
The Gold Coast has numerous restaurants offering a wide variety of cuisines. Many of its entertainment venues offer live music, dancing and gaming areas.
At Broad beach, the Conrad [Jupiters Casino] and newly built [Gold Coast Exhibition Centre] feature excellent eateries, exhibitions, entertainment and live music by recognised artists all year round.
And for nightlife, Surfers Paradise, also known as the party centre of the Gold Coast, hosts a large selection of restaurants, pubs and nightclubs all in one location.
All these sites are within a 15 minute drive of the college!
Living Expenses& Work Limitations
Living costs in Australia will ultimately depend on your own lifestyle and will vary by location. Students who study in Australia have the option of a number of types of accommodation, and can choose to live with other students or by themselves.
The average international student in Australia requires between AUD $16,000 and $20,000 to cover living expenses (food, accommodation, clothing, local transport, telephone, electricity, stationery, and entertainment) during each year of study.
The Department of Immigration and Border Protection [www.border.gov.au]states that you need approximately $18,000 (AUD) per year to study in Australia.
More official information on living costs is available at [www.studyinaustralia.gov.au]
You need to make sure you have enough funds to support yourself when you first arrive. We recommend that you have approximately $2000 Australian dollars available for the first three weeks to pay for temporary accommodation and transport.
It is important to note that that most costs are estimated over a weekly period.
Under the conditions of an Australian student visa, you may work up to 40 hours every fortnight. Additionally, during holiday periods you are allowed to work unlimited hours per week.
Read more about the mandatory conditions that are attached to all primary student visas here.
Many types of accommodation are available on the Gold Coast. There is something to suit everyone from inner-city apartments and shared accommodation to luxurious villas on golf courses.
As a guide, here are some approximate* costs associated with living in Australia:
- Hostels and Guesthouses – $90 to $160 (AUD) per week
- Shared Rental – $120 to $280 (AUD) per week
- Homestay – $110 to $270 (AUD) per week
- Rental – $165 to $440 (AUD) per week
*Reference: http://www.openconexus.com/australia/prices-and-living-costs/
Refer to the following Gold Coast accommodation websites for more information:
[Flatmates.com.au] – Australia's leading shared accommodation website
[2stay.com.au] – International student and visitors accommodation group
[varsitytowers.com.au] – A student accommodation building 10 minutes from the ACIT campus
[varsityshores.com.au] –Short & Long term student accommodation facilities
[studygoldcoast.org.au/live/] – An accommodation assistance blog on the Gold Coast
A more comprehensive listing of rental accommodation is available in local newspapers such as the [Gold Coast Bulletin]or the [Courier Mail].
For more inquiries please contact the ACIT Accommodation Officer through our [contact us] form.


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An acousto-optically Q-switched Nd:YVO4/KTiOAsO4 (KTA) intracavity optical parametric oscillator (OPO) is efficiently realized in singly resonated scheme. With an end-pumping diode power of 25.9 W, output signal (1535 nm) power of 3.77 W and idler power (3467 nm) of 1.18 W are obtained at a pulse repetition rate of 50 kHz. A rate-equation model is set up to simulate the output power and time characteristics of both signal and idler waves. And both the numerical and experimental results show that the idler pulse width is shorter than the signal one in a singly resonant OPO.
© 2012 OSAFull Article | PDF Article
OSA Recommended Articles
Haiyong Zhu, Ge Zhang, Huibin Chen, Chenghui Huang, Yong Wei, Yanmin Duan, Yidong Huang, Hongyan Wang, and Gang Qiu
Opt. Express 17(23) 20669-20674 (2009)
R. F. Wu, K. S. Lai, H. F. Wong, W. J. Xie, Y. L. Lim, and Ernest Lau
Opt. Express 8(13) 694-698 (2001)
Jieguang Miao, Jiying Peng, Baoshan Wang, and Huiming Tan
Appl. Opt. 47(23) 4287-4291 (2008)


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Adoption requests → Tokyo Ravens Wiki
What is your username? User:Abbynanodesu
Please link to the wiki here: w:c:tokyo-ravens
How many edits have you made on that wiki? 27 and will keep on rising as time goes
How many days/months have you been editing there? Literally four months
On the Special Pages → Special:ListUsers when was the last time an admin edited, and who was it? It's User:Gcheung28 so I don't care
Any other information: I will seriously do my best!


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Ah, the miracle of life. So graphic, so delicious.
Why hello there, face that will haunt my nightmares forever.
Is the Cat in the Hat the father, or just a supportive friend?
Educational AND delicious!
While not inaccurate, this one just seems to put emphasis on the wrong part of the experience.
Wait, is that the PLACENTA IN THAT BOWL?!
Never have sprinkles been so unappetizing.
Nice bowtie?
Forget the fact that he's sitting on a television. Why does baby Ethan look like an orange E.T.?
Dibs on the head!
I guess that's one way to tell the world you're having twins…
Most terrifying use of jello ever? Most definitely.
The miracle of life, as explained by a teenage boy.
So many questions, beginning with the Playboy bunny, and ending with the flesh window.
The logic of this one is undeniable.
It's like an elegant army invading the castle of creepy.
Tristan's quite a looker. Those eyes!
I guess it's good to consider other possibilities for birth, too…
Wait, why "farewell"? Also, why the sexy lingerie?
Come on in, the water, ahem, placenta's fine!
That's one way to ensure people look at boring photos of your child.
Maybe push a little less, Nicole, lest the baby hits the wall.
Childbirth meets the Exorcist meets your worst fears realized.
Wait, is it climbing out of the bellybutton?
The sign's a nice touch.
That's one happy face for someone surrounded by the world's most horrifying use of strawberries.
They're like a little army of deliciousness coming for you…whether you like it or not.
Is Kristin the mother, or just someone with good hand-eye coordination?
Everybody loves a good meat baby
This is truly the spirit of the occasion, boiled down into one sentence.
Via distractify.com
(Visited 166 times, 1 visits today)


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Users are shaping tomorrow's technology, as they find new ways to use gadgets and services that sometimes even the vendors didn't envision.
"People are in fact a kind of killer app," Tim O'Reilly said in his "O'Reilly Radar" keynote address at the recent Emerging Technology Conference in San Diego. He cites the recent blossoming of so-called social networks as one of the latest examples of user influence on applications.
Online Tribes Emerge
Many people have found both personal and professional use for online networking services such as Friendster, Orkut, and other Web sites designed to help people connect with friends and friends of friends. But the concept of social software goes beyond connecting birds of a feather, O'Reilly says.
He points to Amazon.com's Listmania, which lets people create lists of their favorite books and resources on various subjects, as a practical--and successful--example of the phenomenon.
"User contributions are critical to a product's success," O'Reilly said.
While Friendster and other networking sites have had no trouble attracting participants and investors, many analysts wonder how the services will make money.
Some social networking sites plan to add fee-based premium services. Whether the service is for business or pleasure, succeeding with subscription programs will be tricky, according to observers. Others question the security of such services and warn people away from them for privacy reasons.
New Network Security
It could simply be that new forms of socializing require new rules. Robert Kaye, who runs the MusicBrainz.org music-sharing site, proposes an acceptance system whereby "tribal elders" decide who is safe to allow onto the network, and who isn't.
Kaye proposes a two-tiered system. A server component would simply validate members without knowing the purpose of the network, and a client component would handle searching and trading for files. An open question about such a system is how to balance accessibility and security: The looser the network's policies, the less secure it is; but the more secure the network, the more restrictions are placed on its members, according to Kaye.
Still, the popularity of the services indicates the providers have incentive to tackle these and other challenges in maintaining and expanding the online networking.
Doing the Dirty Jobs
While the Emerging Technology Conference focused on the people side of technology, the keynote address by iRobot Cofounder and President Helen Greiner took a decidedly different approach.
IRobot's products are designed to do the jobs that humans don't want. Greiner said U.S. soldiers in Afghanistan quickly became accustomed to having the company's PackBots inspect caves abandoned by the enemy before sending in troops.
Several Japanese companies are also exploring such industrial uses of robots.
Closer to home, iRobot's Roomba automatic floor vacuum is one of the more practical applications of the company's robotics technology. Roomba's laser-guidance system detects walls and surface edges in order to keep the unit from damaging itself. Greiner says the device needs to remain affordable enough to compete with conventional vacuum cleaners.
IRobot is working with the Defense Advanced Research Projects Agency on several more specialized robots. However, Greiner expects much of the work will have civilian applications, particularly in the area of assistance for elderly and disabled people. It may not be long until you can have a version of the Jetsons' Rosie for your very own.


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Objects Without Meaning Black Cami
Objects Without Meaning Black Cami
A classic black camisole just right for laying or wearing alone. SPECS: -classic cami -straight cut body -slim spaghetti straps -fully lined -hand wash cold -runs true to size, model is wearing size small -made in the USA
Est 2012: Olive is a boutique in East Austin, Texas that features a stock of artful independent design with creative everyday dressing in mind. Olive is a space for discovery and self expression.
Alexandra Michelle's approach to fashion design has never really been about the garment. After working in the industry for over a decade, she launched Objects Without Meaning in Los Angeles in 2011 with the purpose of creating clothes that emphasize the person wearing them rather than the clothes themselves. Instead, she focuses on materials with a natural simplicity and collections that rely on your personal style, not on trends.
Do you have questions about this product? We're here to help!


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Tree Services
Brushbusters - since 1992 serving central Arkansas. Have trees down from the storms? We can cut them up and pile for you to burn, or haul them away. Big Oaks down? We cut, split and stack it for your firewood. Brush Hogging, smooth your gravel drive & add gravel where needed, all types of tractor work and yard services. Reply to this post - booking now ! Call 4WD Tractor with Front End Loader, Bushhog, box blade, disc, etc. Chain saw work, trailer to haul away. Call today!


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HASP server: a database and structural visualization platform for comparative models of influenza A hemagglutinin proteins
Structural analysis
Influenza A viruses possess RNA genomes that mutate frequently in response to immune pressures. The mutations in the hemagglutinin genes are particularly significant, as the hemagglutinin proteins mediate attachment and fusion to host cells, thereby influencing viral pathogenicity and species specificity. Large-scale influenza A genome sequencing efforts have been ongoing to understand past epidemics and pandemics and anticipate future outbreaks. Sequencing efforts thus far have generated nearly 9,000 distinct hemagglutinin amino acid sequences.
Comparative models for all publicly available influenza A hemagglutinin protein sequences (8,769 to date) were generated using the Rosetta modeling suite. The C-alpha root mean square deviations between a randomly chosen test set of models and their crystallographic templates were less than 2 Å, suggesting that the modeling protocols yielded high-quality results. The models were compiled into an online resource, the Hemagglutinin Structure Prediction (HASP) server. The HASP server was designed as a scientific tool for researchers to visualize hemagglutinin protein sequences of interest in a three-dimensional context. With a built-in molecular viewer, hemagglutinin models can be compared side-by-side and navigated by a corresponding sequence alignment. The models and alignments can be downloaded for offline use and further analysis.
The modeling protocols used in the HASP server scale well for large amounts of sequences and will keep pace with expanded sequencing efforts. The conservative approach to modeling and the intuitive search and visualization interfaces allow researchers to quickly analyze hemagglutinin sequences of interest in the context of the most highly related experimental structures, and allow them to directly compare hemagglutinin sequences to each other simultaneously in their two- and three-dimensional contexts. The models and methodology have shown utility in current research efforts and the ongoing aim of the HASP server is to continue to accelerate influenza A research and have a positive impact on global public health.
Influenza A viruses (IAV) are among the most common causes of human respiratory infections and among the most significant because they cause high morbidity and mortality, both in annual epidemics and in unpredictable pandemics
HA is a glycosylated type I integral membrane protein that functions both as the viral receptor-binding protein and fusion protein. HA recognizes sialic-acid (SA) bound glycans with variable specificity for SA with α2-3 or α2-6 glycosidic linkages; these linkages are critical in determining host species specificity
To date, nearly 9,000 distinct HA alleles have been sequenced
Construction and content
Template preparation
All crystal structures of influenza HAs were retrieved from the Protein Data Bank (PDB) and filtered through the PISCES server
Sequence alignments
All unique HA sequences were downloaded from the Influenza Research Database (8,769 as of June 2011)
Model preparation
A conservative approach was taken in the modeling, where only amino acid types, side-chain conformations, and dihedrals were allowed to change, with insertions and deletions omitted from the model. This approach was chosen because of the high sequence similarity between the query and template HA sequences and the desire to retain as much information from the crystal structure as possible.
The models were created by mapping the query sequence onto the template sequence from the hsp alignment using the fixed-backbone design functionality of ROSETTA3, v3.2
Model quality estimates
To derive semi-quantitative estimates of the quality that can be expected for modeling a given HA sequence, the sequences of the twelve HA crystal structures used as templates were modeled using the server, omitting their structures from the possible templates, and the models were compared to the crystal structures by various criteria (Figure
Figure 1
Rotamer recovery rate, sequence identity, and C-alpha RMSD for model-crystal structure pairs
Rotamer recovery rate, sequence identity, and C-alpha RMSD for model-crystal structure pairs. The amino acid sequences for each crystal structure used as templates for the HASP server were modeled using the HASP server and the resultant models compared to the crystal structure. The identities are the number of identical amino acids between the query, template pair. The rotamer recovery rate is the ratio of residues with correctly modeled side-chains to all residues. The C-alpha RMSDs were calculated between the template structure and crystal structure of the sequence being modeled over all C-alpha atoms included in the model. Rotamer recovery
On a finer scale, the rotamer recovery rate was evaluated as a function of amino acid type (Table
HASP is a web application written in Java 1.6, and is designed with standard client/server architecture. The meta-information from GenBank
Utility and discussion
Main features of the HASP server
The HASP server interface has two primary components, the
To start, a user chooses the parameters of the query in the
Sequences of interest are selected by checking the box in the right-hand column of the search results. In the
Discussion of HASP server use through a case study
In the World Health Organization's Human Animal Interface online database for H5N1 Avian Influenza in Humans, it is reported that there were three deaths from H5N1 in Vietnam in 2003 and twenty deaths in 2004
To narrow the search to HA sequences from H5N1 strains,
Figure 2
Searching for hemagglutinin sequences
Searching for hemagglutinin sequences. (A) Opening view in the Search tab. The
Figure 3
Search results
Search results. A/Viet Nam/1203/2004 (AY818135) and A/chicken/Viet Nam/Ncvd8/2003 (EF541407) are selected and the
In the viewer, a model is displayed for the selected 2003-chicken H5. To compare the two models, the identifiers for both sequences are selected at the left of the sequence alignment, highlighting them in purple and instantiating a side-by-side viewer (Figure
Figure 4
Because HA regulates IAV entry into the cell
The primary aim of the HASP server is to provide a tool for viewing and comparing amino acid changes in HA subtypes at all levels of protein structure, from primary to quaternary, giving a complete and integrated view of those changes to facilitate understanding. Understanding these changes, which determine the efficiency of transmission, pathology, and ecology of these viruses, is of critical and vital importance to global public health.
Availability and requirements
The HASP server is available free of charge as a web application at:
HASP: Hemagglutinin Structure Prediction (server); HA: Hemagglutinin; NA: Neuraminidase; RMSD: Root Mean Square Deviation; hsp: High-scoring segment pair; IAV: Influenza A virus; PDB: Protein Data Bank; GWT: Google Web Toolkit.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
XIA, EJD, JKT, and DEH conceived the HASP server. XIA designed the HASP server, created the modeling pipeline, performed the model quality assessment, and authored the manuscript. JD led the development and implementation of the HASP server. VG assisted JD with the development and implementation of the HASP server. All authors contributed to and edited the manuscript, and approved the final version.
We are grateful to Dr. Yentram Huyen and Mike Tartakovsky for their support of the project. We also thank Dr. Michael Dolan for helpful comments on HASP, and Dr. Meghan Coakley for assistance in writing this manuscript. This research was supported in part by the Office of Science Management and Operations of the National Institute of Allergy and Infectious Diseases. This study utilized the high-performance computational capabilities of the Biowulf Linux cluster at the National Institutes of Health, Bethesda, Maryland. (
Influenza: epidemiology, clinical features, therapy, and prevention
Influenza virus evolution, host adaptation, and pandemic formation
Sialic acid species as a determinant of the host range of influenza A viruses
The influenza virus resource at the National Center for Biotechnology Information
H5 avian and H9 swine influenza virus haemagglutinin structures: possible origin of influenza subtypes
H1 and H7 influenza haemagglutinin structures extend a structural classification of haemagglutinin subtypes
PISCES: a protein sequence culling server
MolProbity: all-atom structure validation for macromolecular crystallography
ROSETTA3: an object-oriented software suite for the simulation and design of macromolecules
BioHealthBase: informatics support in the elucidation of influenza virus hostpathogen interactions and virulence
Basic local alignment search tool
Recent developments in the MAFFT multiple sequence alignment program
Roles of the hemagglutinin of influenza A virus in viral entry and development of antiviral therapeutics and vaccines
Influenza virus hemagglutinin–structural studies and their implications for the development of therapeutic approaches
Computational studies of influenza A virus at three important targets: hemagglutinin, neuraminidase and M2 protein
Autopsy series of 68 cases dying before and during the 1918 influenza pandemic peak
1918 Influenza: the mother of all pandemics


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Chat with Ashley Fox
Welcome to SportsNation! On Tuesday, ESPN.com NFL columnist Ashley Fox stops by to chat.
Fox joined ESPN.com in 2011 after 11 years as a writer and columnist at the Philadelphia Inquirer, where she covered the National Football League, the Philadelphia Eagles and the Philadelphia 76ers. She also has worked at the Louisville Courier-Journal and Sports Illustrated. A North Carolina native, Fox lives in suburban Philadelphia with her husband, daughter and son.
Send your questions now and join Fox on Tuesday at 3 p.m. ET!
More Ashley Fox: Chats | Search | @AshleyMFox
Ashley Fox (2:59 PM)
Hi everybody. Thanks for joining me. What should we talk about?
Derek (Oregon)
Ashley Fox (3:00 PM)
Oh, that. Seriously, I've been writing about this issue for the last month, including today after last night. Hope the two sides can hammered out a new deal soon. Today would be good.
Chris Fiegler (Latham,NY)
Did you think the Last Play of the Game on Monday Night was Pass Interference against #81 Golden Tate instead of a Touchdown?
Ashley Fox (3:01 PM)
Absolutely. The league admitted as much today in their statement about the last play, which I thought was telling.
Rich (New Jersey)
Hi, Ashley - I've watched the replay of the Packers - Seahawks game countless times, will the critics of the replacements refs start pointing to this game the Packers should have that they should bring the real refs back?
Ashley Fox (3:02 PM)
This was one of many. I think the league has been getting hammered pretty good on this issue. It is ridiculous that now a team has lost a game on a play that was called incorrectly. What happens if the Packers miss the playoffs by a game? Not good.
no one ever (Earth) [via mobile]
I thought the replacement refs did a GREAT job last night!
Ashley Fox (3:03 PM)
Exactly. Even the POTUS has weighed in on Twitter.
Kevin Barineau- Ocala, Florida [via mobile]
New Jeopardy Episode.... Alex Trebek: for $1000.00 "Three Blind Mice" Contestant: "Alex, "What are NFL Referees" Alex Trebek: "CORRECT!
Ashley Fox (3:03 PM)
Good one.
Rich (New Jersey)
Hi, Ashley. John Fox - $30,000 & Jack Del Rio - $20,000 fines, harsher fines for Bill Belichick, Kyle Shanahan
Ashley Fox (3:06 PM)
I would think so. Maybe double for Belichick. He actually put his hands on an official. Fox and Del Rio were fined for their words, not for touching an official. And Shanahan will probably get popped pretty good, too. I'm pretty certain the league will again remind coaches that it doesn't want them barking incessantly at the refs.
Tim (Seattle) [via mobile]
Will a replacement ref end up with government protection if this keeps going on?
Ashley Fox (3:08 PM)
Maybe. My colleague Darren Rovell had an interesting piece today that the outcome of the Packers-Seahawks game had a $150-250 million shift in bets because the Packers didn't cover the spread. A botched call cost a lot of people a lot of money. Check out that story if you have time. It's fascinating.
John (Wisconsin)
How has the league come to this? this is absolutely horrible. 1 team officially lost due to the refs. Multiple teams had major issues in their games that helped them lose. How can the NFL and the NFL owners not be concerned. I am hearing a lot of fans saying they are getting so disgusted that they don't even want to watch anymore.
Ashley Fox (3:10 PM)
I'm hearing this same sentiment, but the TV ratings are still up and the stadiums, for the most part, are still full. The NFL isn't going to budge on the issue of pensions for part-time employees. I don't blame the league on this. There has to be some middle ground there somewhere.
Andrew (WI)
True leaders own up to mistakes, makes public concessions, and at least says he or she is working to correct the mistakes. The NFL and Goodell instead released a statement where they actually didn't address the most controversial part of the call from last night. Roger Goodell is not acting like a leader. Even if you can't end the lockout, admitting mistakes would go a long way towards reconciliation.
Ashley Fox (3:11 PM)
Agreed. Okay, now on to some football-related questions...
TK (Philladelphia)
OK OK OK, enough about last nights game... sportscenter spent 45 mins of an hour show on it... I counted an they replayed the down 17x.... Will the real Eagles please stand up..... most likely to happen in your opinion Ash... Eagles win the Superbowl or the Browns lose all 16?
Ashley Fox (3:13 PM)
Eagles win the Super Bowl? Wow. You're asking that today? I still think the Eagles get it together. The defense is tough. The turnovers obviously are a killer and the quarterback needs to play smarter and cut down the mistakes. But he and Andy Reid are really getting it this week. If they don't bounce back against the Giants it's going to get ugly.
Michael (Cincinnati)
How close can the Browns make it Thurs night? It clearly looked like the team took a step back at home against Buffalo. Can they find anything on the road against Baltimore?
Ashley Fox (3:14 PM)
It's going to be tough. I'm high on the Ravens. They've won 11 or 12 consecutive home games. Flacco played well after a slow start against the Patriots. I don't see Cleveland being able to keep up with them.
Keenan (Atlanta, GA)
Was Mike Mularkey leaving Atlanta the best offseason move in the NFL? His offense was boring and predicatable and now with him gone the Falcons are finally moving the ball and showing the efficiency on offense many expected the past two years.
Ashley Fox (3:18 PM)
It certainly has worked out well for the Falcons switching offensive coordinators. Atlanta is the only team in the league to score a touchdown on its opening possession in each of the three games and they're the only team in the league that hasn't trailed in a game yet. They are getting on top quickly and then putting their foot on their opponents. And the thing is, who on the offense are you going to double? Roddy White? Julio Jones? The future HOF tight end? Try to take one away and another is going to burn you.
Frank (Melbourne, FL)
Hi Ashley! Are Arizona and Seattle serious contenders for the NFC West, or is it still San Francisco's division to lose?
Ashley Fox (3:22 PM)
I think its still San Francisco's to lose, but the division all of a sudden looks much better, doesn't it? Check out our power rankings. San Francisco is 4, Arizona 5, Seattle 10 and St. Louis 25. Who would've thought there would be three NFC West teams in the top 10 at this point in the season? Not me.
Tony (Richmond, CA)
Are the Jets done with Revis now out for the season? Rex Ryan needed a hug during the press conference.
Ashley Fox (3:24 PM)
Ryan needs more than a hug. Everything the Jets do defensively is based on Revis's ability to shut down one side of the field. They don't have that anymore. They have two average starting corners now instead of having the best in the game who made everybody else better. It's a shame for Revis. Hate to see any player lose a season because of an ACL, but really hate to see an elite player suffer that type of injury.
David (Austin)
Hey ashley, I have been living under a rock the last month, can you get me caught up on current events?
Ashley Fox (3:24 PM)
How long do you have?
Jeff (Seattle)
As a Seahawks fan, should I feel guilty about last night's win?
Ashley Fox (3:27 PM)
Guilty? I don't know. Probably. Lucky might be a better word. Or opportunistic. There probably should be an asterisk next to that one.
Steve (Milwaukee)
I understand from a business standpoint that the owners don't want to back down as we all continue to watch, but don't they care about winning? Yes, the normal officials make mistakes too and some have been game altering, but these refs make close games complete tossups. If the Packers had a real owner, especially an outspoken one like Jerry Jones, would this be a bigger deal?
Ashley Fox (3:29 PM)
Sorry, back to the officials, but there are a lot of good points being made. And yes, I agree that it is somewhat of a gift to the league that it was the Packers that this happened to. But this is what the owners want. Jerry Jones said last week that it is only the losing teams that are complaining, which is simply not true, by the way. And you're right: Close games are toss ups.
JJ (Indiana)
Don't you think the real issue is how gambling will be greatly effected by all of this? It sure would be easy for a bad ref to make a "bad call" and make a lot of $$ from some bad people.
Ashley Fox (3:30 PM)
This has crossed my mind and would be a huge problem. Read Rovell's piece.
Otto (Sheboygan, WI)
Legit question: after last night, I have to wonder: what exactly was the background check process for the replacement officials? As a football fan, I hope this is a matter of incompetence and not something more nefarious, but after the "Saints fan" last weekend, and some VERY questionable calls that have swung more than 1 line, I have to ask: they did check out these guys right?
Ashley Fox (3:32 PM)
That is a legit question. The league I believe did background checks, and hopefully they were thorough. But they missed the Facebook stuff and that one official was a professional poker player. Makes you wonder.
Adam (San Diego)
Did you see the report that came out today from the head of the Lingerie Football League saying they fired some of the crews that the NFL is now using because of incompetence? True story!
Ashley Fox (3:32 PM)
I saw something on Twitter about that. Amazing if true.
wyoming [via mobile]
We seem to be blaming the Commish for the problem, he is just a figurehead for the owners who sit in private country clubs racking in the $$
Ashley Fox (3:33 PM)
That is true. He was hired by the owners and works for the owners. He is doing what they want him to do.
Don (Shawano,Wi.)
Everyone is focused on last nights last play. However, there were two blown calls before on the last drive. The roughing the passer call and the pass interference call which put Seattle in position to try the Hail Mary. But to my question, in the NFC the west division has gone from a laughing stock to the strongest division in a short time. Do you see a similar change in the AFC?
Ashley Fox (3:36 PM)
It will be interesting to see if the NFC West stays that high in our power rankings. The NFC as a whole is much stronger then the AFC. As for the AFC West, I've been saying for months how deep that division is now with Peyton Manning in it. I think they'll get it figured out. That was an enormous win for Kansas City to avoid starting 0-3, but they've got some issues. San Diego should be better than they showed against Atlanta and Oakland showed a little life against Pittsburgh. We'll see how it shakes out.
Jeremy (Indy)
A modest proposal... Every team should agree to take a knee on their first possession of next week's game and every subsequent game use an additional series to take a knee until they bring back the real refs. Once the players end up spending a full quarter taking a knee and no one tunes in to the beginning of games, maybe the No Fairness League will lose some cash and end this dispute.
Ashley Fox (3:36 PM)
Interesting proposal.
Isn't it also about time the fans start to take some accountability in the league's mess? Clearly all the owners respond to is dollars. If the fans don't change their behavior, the owners won't change theirs. For all the indignation spewed today, unless each and every one of the (rightly) complaining fans stops watching and paying money to the NFL next week, their complaints are a waste of time and breath. The odds on that happening = nil. And the owners know it.
Ashley Fox (3:37 PM)
This is true. The NFL is printing money.
Bryan (Chicago)
Can the players or coaches strike over this? Can they engage in a slow-down (i.e., refuse to start 1:00 games until 1:10 or take an extra 5 mins at half-time)?
Ashley Fox (3:39 PM)
The players can not strike, per the new CBA. Could they take the field late or do something else? That would be interesting to see. Or maybe the regular officials could come to the realization that they won't get pensions as part-time employees when many full-time employees of the league and of teams don't get pensions any more. What makes the officials so special? They need to be held accountable as well.
Jane (Milwaukee, WI)
Who has the advantage on Sunday? The scorned Packers, or the perhaps even more scorned Saints?
Ashley Fox (3:41 PM)
The Packers, because they have a head coach. And they're playing at home.
Shane (Robins Air Force Base, GA)
YES, officiating has been horrible; YES, we need the regular refs back. However, I think the coaches/players are talking out of both sides of their mouths when they complain about the refs making bad calls, while simultaneously trying to influence the refs into making bad calls when they (the players and coaches) know they are wrong, like Harbaugh getting 5 timeouts or Carroll getting 4. This is not doing something for a "competitive advantage"; it's cheating and a gross lack of integrity. Thoughts?
Ashley Fox (3:43 PM)
I don't disagree, and I've seen this from other people here. The players are flexing the rules because they can. The more nobel thing would be to play within the rules, but you've got to remember this about the NFL. Everybody's paranoid the other guy is going to gain a competitive advantage. So they're all going to try to take advantage of what they can, in this case with flexing the rules. Doesn't make it right, but that's what's happening.
John (Los Angeles)
Let's say the Bucs are getting blown out, 45 seconds left with no time-outs, and the opponent has the ball. Instead of taking a knee, the opponent throws a bomb for a meaningless TD, running up the score even further. Does Schiano throw a postgame tantrum, or does he applaud the opponent for playing a full 60 minutes of football?
Ashley Fox (3:44 PM)
Great question. If I were him in that situation I would hope he would applaud the opponent for playing a full 60 minutes since, you know, that's what his team is doing.
Ryan (San Jose)
My real issue with the refs is they are losing control of the game and players could really get hurt. Like in the Oakland Pittsburgh game when DHB is almost decapitated by Ryan Mundy, with no flag, and the Raiders responded by chop blocking Ziggy Hood and hurting his knee. Player safety is becoming a real issue in this too.
Ashley Fox (3:47 PM)
I agree. Player safety. Integrity of the game. All those things. Lengths of games. They are hard to watch. I was at the Atlanta-Denver Monday night game and it was brutal. And it's tough for players to get into rhythm when the refs stop the game for minutes at a time to make a call. The players don't like it.
Lance (Weston, WI)
I heard that Mike McCarthy was looking in the stands for replacement offensive linemen after the first half(not). But, seriously, what the heck was the O-line doing?
Ashley Fox (3:48 PM)
That's pretty funny. I was really worried for Aaron Rodgers in that first half. Eight sacks. Wow. They didn't help him out much last night.
Jon Chown (California)
Personally, I don't care anymore if the NFL gets its real refs back. This season has been ruined. Screw this season. Whoever wins the Super Bowl should get an asterisk. For this season, I am done watching. Who cares anymore. I may return next season, or I might not. The NFL has pissed me off and I'm done for now.
Ashley Fox (3:49 PM)
I'm starting to hear this more and more...
Kyle (Philadelphia)
If I am the locked out officials, my last offer just came off the table and I'm raising the price of my services. Thoughts?
Ashley Fox (3:50 PM)
Then they'll stay locked out. The NFL isn't budging on the pension.
Ashley Fox (3:50 PM)
Then they'll stay locked out. The NFL isn't budging on the pension.
Joe (Oklahoma)
Ashley, can't help but feel the outrage would have been slightly less if a team like Arizona, Kansas City or some other smaller-market team were on the losing end of last night's call.
Ashley Fox (3:52 PM)
I don't know about that, but the Packers are popular and should be a Super Bowl contender. That certainly factors in. The other thing is it was on national TV so everybody saw it. That didn't help.
Steve (Milwaukee)
We keep putting the labor dispute in the owners hands (who clearly have the most leverage), but what would make the refs break? Is it just going to take time and sufficient lost wages to cave in?
Ashley Fox (3:53 PM)
That would seem to be the league's strategy, to break the union.
Sean Troy (Arizona)
This season has been really exciting with all the refs' incompetence, true. However, this isn't NFL football. I really am not sure why the NFL doesn't seem to see that. The constant chippiness and bickering and growling between the teams is going to eventually lead to a serious brawl, and the NFL will have nobody to blame but itself.
Ashley Fox (3:55 PM)
I completely agree with you and touched on this point in my column today. Things are going to escalate and if it does, it won't be pretty. And that will be a big problem. I hate that players and coaches are calling for flags to be thrown all the time. It's obnoxious, but they are at times influencing what's being called.
John (Wyoming)
Do you think if this keeps up there will have to be some sort of explaination at the end of the season? Case in point they won during the replacement Ref year!
Ashley Fox (3:56 PM)
Yep. Totally. Maybe not if they get this thing settled soon, but if it last half the season or, god forbid, the entire year, it will be the Replacement Ref year. Let's hope we aren't talking about this in January and February.
Ashley Fox (3:57 PM)
Okay, that hour just flew by. There were so many questions I couldn't get to, but I appreciate all of them. Hit me up on Twitter @AshleyMFox and we will continue there. And we'll do it again next week. Hang in there.
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