text:
Systems biology approach to stage-wise characterization of epigenetic genes in lung adenocarcinoma
Systems physiology, pharmacology and medicine
Epigenetics refers to the reversible functional modifications of the genome that do not correlate to changes in the DNA sequence. The aim of this study is to understand DNA methylation patterns across different stages of lung adenocarcinoma (LUAD).
Our study identified 72, 93 and 170 significant DNA methylated genes in Stages I, II and III respectively. A set of common 34 significant DNA methylated genes located in the promoter section of the true CpG islands were found across stages, and these were:
Integrating epigenetic information for genes with expression data can be useful for comprehending in-depth disease mechanism and for the ultimate goal of better target identification.
Cancer progression is associated with mutation and differential gene expression
Advances in next-generation technologies have led to identification of genome-wide DNA methylations in a large number of disease samples. Methylation sites have been analyzed based on clustering with respect to genomic regions, methylation patterns, and common regulatory patterns
Lung cancer is one of the most commonly diagnosed cancers in United States. Lung cancer is morphologically divided into NSCLC and small cell lung cancer (SCLC)
The aim of the study was to elucidate the DNA methylation patterns across different stages of LUAD from publicly available data resources. We used The Cancer Genome Atlas Data (TCGA) as our resource for methylation data. In this study, a systems biology approach of integrating gene-expression, DNA methylation and protein-protein interaction data for finding highly important DNA methylated genes across stages of LUAD were developed. These DNA methylated genes were compared across stages for their uniqueness and commonality to identify the patterns across stages of LUAD. These patterns were then validated and ranked for their importance in LUAD using literature evidences
The objective of this study was to understand the Significant DNA methylated genes across the four stages of LUAD and analyze them as potential targets. The TCGA data associated with LUAD was classified based on these stages. For this stage-wise data, the patient's age ranged from 58–75 with few outliers. The Significant expressed genes and Significant DNA methylated genes were identified based on the
Additional file 1
Click here for file
The Significant DNA methylated genes were compared across stages as shown in Figure
Figure 1
(A) Venn diagram of all DNA methylated genes across stages; (B) Venn diagram of hypermethylated genes across stages; (C) Venn diagram of hypomethylated genes across stages
(A) Venn diagram of all DNA methylated genes across stages; (B) Venn diagram of hypermethylated genes across stages; (C) Venn diagram of hypomethylated genes across stages.
As shown in Figure
Identification of highly scored Significant DNA methylated genes
The significant DNA methylated genes were analyzed and ranked based on their beta-values. Table
Significant DNA methylated genes in and outside of CpG islands, promoter regions, transcription factors, chromosomes and pathways
The hypermethylated and hypomethylated genes were further analyzed with respect to their methylation inside and outside of the CpG islands. Table
Figure 2
Profile of DNA methylated genes as transcription factors across stages
Profile of DNA methylated genes as transcription factors across stages.
Figure 3
Profile of methylated genes
Profile of methylated genes. A Distribution of hypermethylated genes across chromosomes in different stages. B Distribution of hypomethylated genes across chromosomes in different stages.
Additional file
Additional file 2
Pathway distribution for DNA methylated gene across stages.
Click here for file
Network construction and analysis
A systems biology approach was developed to understand the Significant DNA methylated genes and Significant expressed genes in each stage. The interactions for the Significant DNA methylated genes and Significant expressed genes were identified using BioGRID
Figure 4
Stage-wise interactions of DNA-methylated genes with significant genes
Stage-wise interactions of DNA-methylated genes with significant genes.
Additional file 3
Analysis of subnetworks of size 2, 3, 4, and 5 across stages.
Click here for file
Additional file 4
Analysis of common and unique subnetworks of size 4 revealing the significant genes.
Click here for file
Analysis of these subnetworks is an NP-hard problem because these are large open subnetworks. To reduce the complexity, the subnetworks were ranked based on their
Additional file 5
Significant DNA methylated genes in the UBC network.
Click here for file
Figure 5
Circos image showing the stage-wise distribution of hypermethylated, hypomethylated genes and pathway class in the chromosomes
Circos image showing the stage-wise distribution of hypermethylated, hypomethylated genes and pathway class in the chromosomes. Outer circle represents the chromosomes, the first inner circle represents the hypermethylated genes in each chromosome, and the second inner circle represents the hypomethylated genes on each chromosome and inside is the pathway class to which the gene on the respective chromosome belongs.
Figure 6
Comparison of different types of networks obtained from BioGRID and manually curated signaling network
Comparison of different types of networks obtained from BioGRID and manually curated signaling network. The "bio_stage" refers to BioGRID and "sig_stage" refers to signaling network. The Venn diagram depicts the overlap of genes between the two networks obtained from the two different sources, BioGRID and Signaling network. A) Venn diagram of the methylated genes for Stage I, Stage II, and Stage III. (B) Venn diagram of expression genes for Stage I, Stage II, and Stage III. (C) Venn diagram of methylated-expression genes for Stage I, Stage II, and Stage III.
Distribution and understanding of Significant DNA methylated genes across stages
According to Table
Of the 12 common Significant DNA methylated genes common to Stages I and II,
Of the 30 common Significant DNA methylated genes across Stages II and III,
Of the 42 common Significant hypermethylated genes unique to Stages I and III,
Of the four Significant hypomethylated genes in Stage I and III, in addition to
In addition to genes that were found to be common across all or two stages, several genes were unique to one stage only, and these need further evaluation. From Stage I, NEFM has been reported as a biomarker and as a methylated gene in cancer
Distribution of the Significant DNA methylated genes in and outside of CpG islands, promoter regions, transcription factors, chromosomes and pathways
Our initial distribution analysis found that most of the Significant DNA methylated genes across the three stages were present in the TRUE CpG sites (as shown in Table
DNA methylation is closely linked with gene regulation, particularly with transcriptional activity. It has been reported that DNA methylation can prevent gene activation and restrict expression for correct developmental stage
Earlier studies have reported chromosome 6 and 15 to play an important role in lung cancer
The analysis found that chromosomes with the highest number of hypermethylated genes in Stage I also had the highest number of hypomethylated genes in Stage II and eventually have the highest number of hypermethylated genes in Stage III (Figure
The pathway analysis depicted the onset of different epigenetically modified pathways across stages. The common signaling pathway identified across the three stages shown in Additional file
Understanding the DNA methylated stage-specific networks
Effective drug target identification in a disease now requires incorporating knowledge of the epigenetic genes with knowledge of other biological features. Biological networks help understand and elucidate the roles of the molecular entities individually and collectively. Therefore, the epigenetically modified genes identified in our study were further analyzed in terms of their interaction partners across different stages of LUAD. This network analysis can help to recognize patterns that were not visible by exploring the expression data alone and help to illustrate the conserved and unique patterns across the stages of LUAD. These patterns could then be further validated in laboratories for their efficacy as drug targets.
The missing links and novel genes of Table
To understand the commonality and uniqueness of the Significant DNA methylated genes in the context of the other significant expressed genes, we developed and performed subnetwork analysis (as described in the methodology section). Subnetworks of each size were analyzed with respect to their hub genes. Table
Analysis of the genes in the subnetworks (Additional file
The top ranked subnetworks of size four in each stage were propagated based on their
Effectiveness of methodology to extract significant subnetworks for networks obtained using variable dataset sources is proved by the comparison results showed in Table
The study was entirely based on the available TCGA data, which has the limitation of unequal samples; still we were able to prove the advantage of integrating epigenetic data, expression data and protein-protein interaction knowledge for advancing of systematic understanding of LUAD. This understanding can be further improved by incorporating the system biology approach to the epigenetic profile across the different stages of LUAD. The study identified 72, 93 and 170 epigenetic genes across Stages I, II and III. A set of 34 common epigenetic genes were identified across the three stages, and it was observed that methylation patterns were similar across Stages I and III, but were different in Stage II. The study also identified known, and novel epigenetic genes across stages that were important in LUAD, these genes could be further validated in the laboratory for their scope as targets. The novel epigenetic genes identified were
The gene expression and DNA methylation data for LUAD were downloaded from TCGA
The overall methodology for the stage-wise identification of LUAD process is shown in Figure
Step A: the gene expression data from UNC AgilentG4502A_07_3 were analyzed based on the log2 values to obtain the differentially expressed genes.
Step B: the methylation data from Illumina HumanMethylation27 for each stage were analyzed based on the beta value to obtain the differentially methylated genes.
Step C: the data obtained from Step A and B was integrated to obtain a stage-specific network of LUAD. This network was annotated with the topological and biological features for analyzing the methylated patterns.
Step D: the stage-specific subnetworks were obtained for LUAD.
Figure 7
Overall Methodology
Overall methodology. (A) UNC AgilentG4502A_07_03 gene expressed data was analyzed based on the log2 values to obtain the differentially expressed genes. (B) The methylation data from Illumina HumanMethylation27 were classified for each stage. Significantly DNA methylated genes were identified. (C) Stage-specific interaction networks were constructed and annotated with their DNA methylated genes. The nodes and edges of each network were scored based on the topological and biological features. (D) The epigenetic subnetworks were identified and compared across stages to understand the epigenetics commonality and uniqueness.
Following sections contain details of each step.
Step A. Identification of significant genes from expression data
The level 3 data available from TCGA
Step B.1. Identification of significant DNA methylated genes from methylated data
The beta-values
Step B.2. Analysis of DNA methylated genes with respect to the CpG islands, promoter regions, transcription factors, chromosome distribution and pathways
The correlation of DNA methylated genes with CpG islands is assessed by mapping the position of the DNA methylated gene to CpG position using computational methods
Step C. Understanding the stage-specific networks of LUAD
To understand the significance of the DNA methylated genes in LUAD, stage-specific networks were obtained using the following steps:
Identification of gene-gene interactions and DNA methylated-gene interactions from BioGRID and constructing the stage-specific networks of LUAD
The gene-gene physical interactions (associations) for all the Significant expressed genes and Significant DNA methylated genes were identified using BioGRID for all stages
To understand the overall profile of each stage-specific network of LUAD, a system's biology approach was developed. All the nodes (genes) and edges (interactions) were annotated with their respective topological and biological features. The statistical computing tool R (
For a graph
And where
Pathway sifnificance score
The pathways associated with each nodes
For any two interacting nodes (genes) in the network,
All the genes and their edges in the stage-specific network were then annotated with their
Step D. Identification and scoring of epigenetically relevant subnetworks across stages
To compare and elucidate the interaction network of Significant DNA methylated genes across stages is a hard problem. Therefore, the networks were analyzed using graph techniques by identifying the relevant subnetworks
The Significant DNA methylated genes in each stage were ranked based on their beta-value. The Significant DNA methylated gene with the highest beta-value was considered as a SEED. The SEED and expand algorithm was then used to identify the next connecting gene (node) and interaction (edge) based on the
The subnetworks were compared for their commonality and uniqueness across stages to identify those Significant DNA methylated genes that could be potential targets. These were then validated using literature for their importance in LUAD. In order to prove the universal nature of above detailed network analysis, the methodology was repeated for the interacting genes obtained from manually curated Human Signaling Network dataset (
TFs: Transcription factors; NSCLC: Non-small cell lung cancer; SCLC: Small cell lung cancer; LUAD: Lung adenocarcinoma.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
MPP: conceptualizing and developing methodology, analysis of all the algorithm result, writing manuscript. AAD: data collection and analysis, scripting, figures and input for writing. MJP: PI of the project, conceptualizing the objective, writing manuscript, valuable inputs at all the time. All authors read and approved the final manuscript.
Authors' information
Akshay Desai and Meeta Pradhan are co-first authors.
We would like to thank Maya Palakal and Deepali Jhamb for editing the manuscript. We also want to thank all the members of the TiMAP laboratory at IUPUI for their valuable suggestions.
Genetic alteration and gene expression modulation during cancer progression
The origin of oncogenic mutations: where is the primary damage?
Prioritizing cancer-related genes with aberrant methylation based on a weighted protein-protein interaction network
DNA Methylation as Clinically Useful Biomarkers-Light at the End of the Tunnel
DNA methylation analyses of urothelial carcinoma reveal distinct epigenetic subtypes and an association between gene copy number and methylation status
Epigenetics and cancer
Analysing and interpreting DNA methylation data
Epigenetics in Cancer
Genome-epigenome interactions in cancer
Cancer genomics: DNA methylomes and histone-modification maps
Epigenetics and human disease: translating basic biology into clinical applications
Features of methylation and gene expression in the promoter-associated CpG islands using human methylome data
CpGProD: identifying CpG islands associated with transcription start sites in large genomic mammalian sequences
CpG islands or CpG clusters:how to identify functional GC-rich regions in a genome?
Epigenetic gene silencing in cancer: the DNA hypermethylome
A comparative analysis of DNA methylation across human embryonic stem cell lines
Linking the epigenome to the genome: correlation of different features to DNA methylation of CpG islands
Genome-wide analysis of promoter methylation associated with gene expression profile in pancreatic adenocarcinoma
DNA hypermethylation in tumorigenesis - epigenetics joins genetics
An integrative analysis of DNA methylation and RNA-Seq data for human heart, kidney and liver
Identification of aberrant chromosomal regions from gene expression microarray studies applied to human breast cancer
DNA methylation profiling in breast cancer discordant identical twins identifies DOK7 as novel epigenetic biomarker
Promoter hypermethylation and BRCA1 inactivation in sporadic breast and ovarian tumors
Genome-wide DNA methylation profiling of non-small cell lung carcinomas
Overexpression and hypomethylation of flap endonuclease 1 gene in breast and other cancers
TKTL1 is activated by promoter hypomethylation and contributes to head and neck squamous cell carcinoma carcinogenesis through increased aerobic glycolysis and HIF1alpha stabilization
Methylation markers of early-stage non-small cell lung cancer
Integrated analysis of genetic and epigenetic alterations reveals CpG island methylator phenotype associated with distinct clinical characters of lung adenocarcinoma
Genome-wide CpG island methylation analyses in non-small cell lung cancer patients
The human disease network
A direct approach to false discovery rates
DNA methylation landscapes: provocative insights from epigenomics
BioGRID: a general repository for interaction datasets
g:Profiler - a web-based toolset for functional profiling of gene lists from large-scale experiments
g:Profiler–a web server for functional interpretation of gene lists (2011 update)
HOX genes in pancreatic development and cancer
Lineage infidelity of epithelial ovarian cancers is controlled by HOX genes that specify regional identity in the reproductive tract
Detection of HOXA9 gene methylation in tumor tissues and induced sputum samples from primary lung cancer patients
Homeobox gene methylation in lung cancer studied by genome-wide analysis with a microarray-based methylated CpG island recovery assay
Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes
Genome-wide screening of genes regulated by DNA methylation in colon cancer development
Frequent and simultaneous epigenetic inactivation of TP53 pathway genes in acute lymphoblastic leukemia
DNA methylation markers predict outcome in node-positive, estrogen receptor-positive breast cancer with adjuvant anthracycline-based chemotherapy
Ectopic Tbx2 expression results in polyploidy and cisplatin resistance
Methylation status of NEUROG2 and NID2 improves the diagnosis of stage I NSCLC
DNA methylation biomarkers for lung cancer
Non-small cell lung cancer invasion and metastasis promoted by MMP-26
GeneCards: Integrating information about genes, proteins and diseases
Genomic deregulation during metastasis of renal cell carcinoma implements a myofibroblast-like program of gene expression
Genome-Wide Analysis of DNA Methylation and the Gene Expression Change in Lung Cancer
Leptin and leptin receptor polymorphisms are associated with increased risk and poor prognosis of breast carcinoma
Neuromedin U is overexpressed in pancreatic cancer and increases invasiveness via the hepatocyte growth factor c-Met pathway
Deletion or epigenetic silencing of AJAP1 on 1p36 in glioblastoma
Association analyses identify multiple new lung cancer susceptibility loci and their interactions with smoking in the Chinese population
A multilevel data integration resource for breast cancer study
Gene amplification at chromosome 1pter-p33 including the genes PAX7 and ENO1 in squamous cell lung carcinoma
Epigenetic stem cell signature in cancer
SOX17 methylation inhibits its antagonism of Wnt Signaling pathway in Lung cancer
Minimal deletion regions in lung squamous cell carcinoma: association with abnormality of the DNA double-strand break repair genes and their applications on gene identification and prognostic biomarkers
Epigenomic analysis of aberrantly methylated genes in colorectal cancer identifies genes commonly affected by epigenetic alterations
Genome-wide methylation analysis identifies epigenetically inactivated candidate tumour suppressor genes in renal cell carcinoma
Identification of novel neuroendocrine-specific tumour genes
CpG island hypermethylation in human astrocytomas
Effects of human papillomavirus (HPV) type 16 oncoproteins on the expression of involucrin in human keratinocytes
Interplay between DNA methylation and transcription factor availability: implications for developmental activation of the mouse myogenin gene
DNA methylation has a local effect on transcription and histone acetylation
Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR
Forkhead box O-class 1 and Forkhead box G1 as Prognostic Markers for Bladder Cancer
Expression analysis of angiogenesis-related genes in Bulgarian patients with early-stage non-small cell lung cancer
HOX genes in ovarian cancer
Abnormal methylation of seven genes and their associations with clinical characteristics in early stage non-small cell lung cancer
Plasma soluble HLA-G is a potential biomarker for diagnosis of colorectal, gastric, esophageal and lung cancer
KEGG for integration and interpretation of large-scale molecular datasets
The role of BCL11B in hematological malignancy
Aberrant promoter methylation and expression of UTF1 during cervical carcinogenesis
EMX2 is epigenetically silenced and suppresses growth in human lung cancer
Identification of known and novel pancreas genes expressed downstream of Nkx2.2 during development
A multi-factorial signature of DNA sequence and polycomb binding predicts aberrant CpG Island methylation
Hypermethylation of a cluster of Kruppel-type zinc finger protein genes on chromosome 19q13 in oropharyngeal squamous cell carcinoma
Epigenetic silencing of erythropoietin in human cancers
Prognostic Significance of Gremlin1 (GREM1) promoter CpG Island hypermethylation in clear cell renal cell carcinoma
Frequently methylated tumor suppressor genes in head and neck squamous cell carcinoma
A major lung cancer susceptibility locus maps to chromosome 6q23-25
Chromosome 15q25 (CHRNA3-CHRNA5) variation impacts indirectly on lung cancer risk
Large-scale delineation of secreted protein biomarkers overexpressed in cancer tissue and serum
Gene amplifications at chromosome 7 of the human gastric cancer genome
Chromosome 7 abnormalities in prostate cancer detected by dual-color fluorescence in situ hybridization
The prognostic value of chromosome 7 polysomy in non-small cell lung cancer patients treated with gefitinib
p53 alterations and chromosome 17 aberrations in non-small cell lung cancer
Current status of human chromosome 14
Mouse Lbx1 and Human Lbx1 define a novel mammalian homeobox gene family related to the drosophila lady bird genes
Identification of driver and passenger DNA methylation in cancer by epigenomic analysis
Detection of bladder cancer using novel DNA methylation biomarkers in urine sediments
Transcriptional profiling of non-small cell lung cancer cells with activating EGFR somatic mutations
Lung cancer and Toll-like receptors
Altered death receptor signaling promotes epithelial-to-mesenchymal transition and acquired chemoresistance
Activation of focal adhesion kinase in human lung cancer cells involves multiple and potentially parallel signaling events
Sonic hedgehog pathway expression in non-small cell lung cancer
A systems biology approach to the global analysis of transcription factors in colorectal cancer
PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma
Genetic alteration of the beta-catenin gene (CTNNB1) in human lung cancer and malignant mesothelioma and identification of a new 3p21.3 homozygous deletion
Heterogeneities in the biological and biochemical functions of Smad2 and Smad4 mutants naturally occurring in human lung cancers
Zyxin is a TGF-β/Smad3 target gene that regulates lung cancer cell motility via integrin α5β1
TP53 mutations in nonsmall cell lung cancer
WNT/TCF signaling through LEF1 and HOXB9 mediates lung adenocarcinoma metastasis
Androgen receptor and androgen-dependent gene expression in lung
MYC-induced epigenetic activation of GATA4 in lung adenocarcinoma
Amplification and overexpression of SKP2 are associated with metastasis of non-small-cell lung cancers to lymph nodes
Coordinated regulation of ATF2 by miR-26b in gamma-irradiated lung cancer cells
Altered pattern of Cul-1 protein expression and neddylation in human lung tumours: relationships with CAND1 and cyclin E protein levels
Integrative genome analyses identify key somatic driver mutations of small-cell lung cancer
Identification of reference genes across physiological states for qRT-PCR through microarray meta-analysis
Identification of Importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens
BRAF and RAS mutations in human lung cancer and melanoma
PIK3CA mutation and amplification in human lung cancer
Genetic and bioinformatic analyses of the expression and function of PI3K regulatory subunit PIK3R3 in an Asian patient gastric cancer library
MicroRNA-7regulated TLR9 signaling enhanced growth and metastatic potential of human lung cancer cells by altering PIK3R3/Akt pathway
Cancer Genome Atlas
A map of human cancer signaling
Regulatory network motifs and hotspots of cancer genes in a mammalian cellular signalling network
The human phosphotyrosine signaling network: Evolution and hotspots of hijacking in cancer
Construction of human activity-based phosphorylation networks
Removing batch effects in analysis of expression microarray data: an evaluation of six batch adjustments methods
Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysis
Historical notes on the Wilcoxon unpaired two-sample test
An introduction to the bootstrap - Efron, B, Tibshirani, RJ
KEGG: kyoto enclyopedia of genes and genomes
Collective dynamics of 'small-world' networks
GOSemSim: an R package for measuring semantic similarity among GO terms and gene products
Interactome networks and human disease
Network-based classification of breast cancer metastasis
Dominating biological networks


text: Great Italian War Battle of Pavia (1512) Beginning: 1508 End: 1512 Place: Italy Outcome: Italo-Spanish victory; Treaty of Lyon Combatants Spain Papal States Duchy of Milan Swiss Confederation France Republic of Venice Duchy of Ferrara Kingdom of Naples Commanders Ferdinand II of Aragon Julius II Maximilian Sforza Matthaus Schiner Charles VII Leonardo Loredan Alfonso I Ferdinand of Naples Strength 240,000 300,000 Casualties and Losses 48,000 67,000 Contents[show] Background French Invasion: 1508 The Campaign Drags On: 1509 The Napalese Campaign: 1510 Spanish Counterattack: 1511 Invasion of Savoy: 1512 Treaty of Lyon Retrieved from "http://althistory.wikia.com/wiki/Great_Italian_War_(The_Habsburgs_Shall_Prevail)?oldid=524160" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: The Habsburgs Shall Prevail Wars Wars (The Habsburgs Shall Prevail)

subset: commoncrawl


text:
Discussion in 'Off Topic' started by steven s, Nov 26, 2011.
Attempted to update a forum I'd rather not mention.
But it does make me feel a little better now.
I did that a day or so ago, created more issues than it solved :nod:
Does it begin with a letter between u and w?
You can sell your license on my site, it has a marketplace.
Let it be some other idiots problem.
That way you can stop being the idiot falling for the shenanigans thinking that upgrading non-xenforo software will improve your site.
Ah yes, the time of backing up everything, doing a testupgrade, updating all addons (because everything always breaks anyway) fixing everything manual, doing the actual upgrade, fixing everything again, noticing newly introduced bugs, getting about 4 mails about yet another very important security patch because the FAQ suddenly gives away your MySQL password...
Good times.
I think we all know how this thread is going to go, so probably best to close it now.
Separate names with a comma.


text:
There's been a bit of a resurgence recently in the debate about the merits of the whiteboard interview – that it's beyond out of date and a model for perhaps the worst way to evaluate software engineering talent. Of course, the most common response to this criticism is, "Well, this is just the way we've always done it."
Ok, full disclosure: We do use the whiteboard at Hedvig in hiring, but it's hardly the be-all and end-all of the interview process. It's simply one part of many in finding the best fit between Hedvig and job candidates. If correctly structured, the algorithm problems and coding challenges will give meaningful information to both the prospective candidate (these problems shed light on the type of work a candidate will see when they join the team) and to my team.
Here at Hedvig we like to know more about candidates and their thinking style rather than just testing their abilities, so we stay away from rote examinations that other companies often use to winnow out as many candidates as possible. I've interviewed hundreds of engineering candidates over the course of my 20-plus year career, and with that experience, I've found it's best always to include the following four particular questions in each and every interview.
Question 1: What is your most successful project to date, and why was it the most successful?
We ask this question to understand the candidate's thought process. The project itself is actually irrelevant; what's key is the ability to articulate technical information in a group setting and to demonstrate that the person can think clearly about why something worked out well.
One of our engineers once asked me, prior to interviewing a very senior candidate, "How do I really interview this candidate? He knows more about 'xyz' subject than I ever will." My answer was simple: If the candidate can describe a complex project so that you clearly understand its value and its concept, you have proved that you can learn from the candidate as well.
Also, candidates who attribute their success to their team rather than to their own singular brilliance are often better able to adapt to our collaborative environment. At Hedvig, we shy away from developers who aren't team players and instead fancy themselves rockstar coders.
Question 2: Have you worked with any distributed systems and, if not, what is your comfort level around these concepts?
Our founder and CEO, Avinash Lakshman, built the company with the focus on bringing a distributed systems mindset to storage, an industry that has seen only incremental innovation over the last decade. As a result, everything we do at Hedvig is built around distributed systems and our most senior engineers know them like the back of their hand.
We recognize that few new developers and computer science graduates come to us already steeped in all things distributed computing. Even experienced developers may not have distributed systems experience. But we do expect our candidates and employees to have at least a passing knowledge of technologies such as Apache Cassandra and Dynamo. Put simply: distributed systems as an architecture does not mean you can run your application across multiple nodes, and we definitely don't consider client/server architectures as distributed. A sophisticated and nuanced understanding of distributed systems comes in time, and we're big proponents of learning on the job. We're looking for those with a passion for doing things in new ways.
By the way, it's usually at this point that a candidate will jump up to the whiteboard (or be prompted to do so) to illustrate distributed systems concepts.
Question 3: Are you comfortable talking with customers?
Even though we're fortunate to have won large, enterprise customers, we're still a scrappy startup. Avinash can often be found side by side with our engineering team, working out the kinks or optimizing a customer's deployment of the Hedvig Distributed Storage Platform. In short, we wear many hats. Our engineers also assist with customer service and are often on the phone with customers to troubleshoot any issues. This is a valuable loop that enables us to build loyalty among our users and receive direct feedback to improve the product.
Question 4: What is an example of a project where you failed or did not have a successful outcome, and what would you have done differently?
This is the final question that I usually ask to bookend the interview. It is very much about ferreting out how the candidate thinks rather than what she or he has done. In addition, the candidates we hire have already learned how to admit mistakes, learn from them and take that knowledge into new projects and endeavors. We look for people with an insatiable desire to keep learning and improving their programming skills.
Everyone fails at some point, and the lessons you learn by picking yourself up and starting again often help push you to the next big achievement or breakthrough. At Hedvig, we're doing something that hasn't been done before, and we need folks who are OK with taking the risks associated with such innovation.
Not the be-all and end-all
So why do we value whiteboard skills when they've suddenly become so passé? This is partly because our engineers are often also teachers – sometimes explaining their code line by line in group code reviews. Or check out this pic of Avinash holding court with some of our engineers as he whiteboards distributed systems concepts.
As a result, we often see more extroverted applicants do a bit better in this portion of the interview. At the same time, whiteboards are by no means the only criterion on which we evaluate people. To be flexible, we often also give a homework assignment so candidates can spend time on a problem at their own pace.
I've even had a few engineers ask not to use a whiteboard and instead offer to walk me through a side project they've built themselves. And that's fine, too. As Jon Evans at TechCrunch wrote last year, "Every candidate must have a side project that they wrote, all by themselves, to serve as their calling card."
Again, the whiteboard and the side project are tools for me to better understand how candidates think and why they did what they did and not just focus on the end result. So if you think you have what it takes to answer these questions — with or without a whiteboard — then apply today!


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|David Jencks <david_jen...@yahoo.com>
|Re: [Discuss] Model for deployment plans in GEP - Move from EMF to XMLBeans or JAXB?
|Fri, 14 Mar 2008 19:47:57 GMT
On Mar 14, 2008, at 3:54 AM, yunfeng ma wrote: > Cool, great news ... :) > > I'm trying to replace EMF using JAXB and got some issues need your > help. > 1. Some elements in the deployment plan can not be unmarshalled, > such as gbeans in geronimo-web.xml. I'm not sure if it's because > geronimo-web-2.0.1.xsd only includes an abstract element "service" > which is the parent of "gbean". > Is the gbean in the correct namespace for the schemas involved? In the geronimo plan processing we do quite a bit of work to correct namespaces so that the plan author doesn't need to keep changing namespaces; in particular we set the correct namespace for gbeans no matter what the original namespace is. > 2. Now the schema files are compiled to generate the model classes > during every GEP building. I think the better way is generating the > java source codes offline and importing the source codes to svn > directly, this provides us a chance to customize the generated > source codes (like the way in OpenEJB). I agree. > 3. The deployment plan saved by JAXB after modification is > unformatted, only contents are in one line. We need save the > deployment plan with well format. This code seems to work well in AttributesXmlUtil: public static void writeAttributes(AttributesType metadata, Writer out) throws XMLStreamException, JAXBException { Marshaller marshaller = ATTRIBUTES_CONTEXT.createMarshaller(); marshaller.setProperty("jaxb.formatted.output", true); JAXBElement<AttributesType> element = new ObjectFactory ().createAttributes(metadata); marshaller.marshal(element, out); } > 4. More elegant way to operate the POJOs generate by JAXB. JAXB > uses POJO to represent the element in XML, so how to create a new > instance of an element (JAX > B way is too fussy), how to set/get the properties of an element > via property name (JAXB doesn't provide any way for this), how to > get the parent instance of an element (JAXB doesn't provide any way > for this) etc. > I don't understand the problem here. I thought the main point of using something like jaxb was so you can write typed java code for a typed java model corresponding to the xml. Do you want to write generic java code to deal with the typed java model? thanks david jencks > Any advice for the above issues? > Thanks a lot. > > -- YunFeng Ma
|View raw message


text: Dream Thrush Printings/Rarity: Cost: CMC: 2 Card Type: Creature — Bird Power/Toughness: 1/1 Oracle Text: Flying : Target land becomes the basic land type of your choice until end of turn. Flavor Text: Whether for good or ill, their arrival always means change. Retrieved from "http://mtg.wikia.com/wiki/Dream_Thrush?oldid=49315" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Invasion Common Cards CMC: 2 Blue Creature Bird Flying Mana Denial


text:
Reader: Ballpark Neighborhood Used to Smell Like Desperation...
Coors Field under construction in 1994.
Jonathan Shikes
Twenty years ago, Denver native — and now Westword managing editor — Jonathan Shikes snapped photos of under-construction Coors Field, going up just north of LoDo in an old warehouse area often called NoDough, given the lack of economic activity there. Today the Ballpark neighborhood is not just a go-to spot on game day, it's one of the city's hottest dining destinations, with big residential complexes popping up all over. Are the changes over the past twenty years an improvement? Observes Miles:
Used to smell like desperation. Now it smells like pee.
Says Johnny:
That neighborhood definitely got a major facelift, but the roads are narrow, there is never enough parking and oh, by the way, we put everything into these stadiums while homeless people sit without proper shelter and food half the time from just two blocks away.
Concludes Jack:
The past 20 years hasn't helped my looks any.
What do you think of the transformation of this neighborhood? Work & Class, which opened in January 2014, just won our Best New Neighborhood Restaurant award in the Best of Denver 2015; what's your favorite restaurant in the Ballpark neighborhood?
Related Location
2500 Larimer St.
Denver, CO 80205
Get the Food & Drink Newsletter
Our weekly guide to Denver dining includes food news and reviews, as well as dining events and interviews with chefs and restaurant owners.
Recommended for You
Powered by SailThru
Popular Stories
Sponsor Content


text:
Twitter competition to win £100 gift card from The Gift Card Centre
Sunday, 8 November 2009 | Admin
TheGiftCardCentre.co.uk's Twitter Feed Allows Customers to Keep Up-to-Date With New Gift Cards and e-Gift Vouchers Available to Buy Online
UK's first online gift card superstore engages with customers on Twitter, giving away £100 gift card to one lucky Twitter follower this Christmas
Gift cards are constantly being added at the UK's top gift card and e-gift voucher shopping portal TheGiftCardCentre.co.uk . And now customers can keep up-to-date with new gift cards, gift card news and special offers at TheGiftCardCentre's Twitter feed, found at hhttp://twitter.com/giftcardcentre
"Twitter gives us the platform to keep instantly in touch with our customers, which is important in such a fast-paced and growing industry," said Keith Brand, Director of Marketing for TheGiftCardCentre.
"We see Twitter as just one more convenient consumer touch-point, and we're even giving away a £100 gift card to one of our lucky Twitter followers for Christmas."
UK-based TheGiftCardCentre.co.uk is the first website dedicated to selling gift cards online. Gift cards and vouchers in the UK represent over £3 billion in turnover, and TheGiftCardCentre is the UK's one and only online gift card superstore.


text:
Project Lead jobs in Alexandria, LA
Showing jobs 1–2
Newest | Relevant first
Sort by: Date posted | Relevance
Crest Industries | Pineville, LA
Construction ManagerProvide support to project team by review of project documents and by maintaining and communicating field changes.
5 - 7 years experience|
Manufacturing / Electronics
Building Materials Manufacturer | Alexandria, LA and 78 other locations
Industrial Maintenance Manager$70K - $90KIdentifies and executes development for the maintenance team in both technical and soft skills; ensures the sustainability of completed improvements by the maintenance team.
5 - 7 years experience|
Building MaterialsPosted by Phil Rosenberg


text:
"It is true that you can use any 2.5 laptop SATA hard drive & just format it on the system, instead of having to buy specific drives made just for it like you do with Xbox 360. Also is it true you can buy games on market place, and not have to pay to play online as you do on 360? Otherwise what are the differences other then the blue ray on PS3?"
Bought this for my husband, and he loves it as do my sons. We love the fact that it is also a blue ray dvd player. It is slim and awesome to play.I just wish we had a option to pick what game it came with....
PS3 - 320gb Uncharted 3: Drake's Deception Bundleby Sony Computer Entertainment
ITEM#: 13883642PS3 - 320gb Uncharted 3: Drake's Deception Bundle. PlayStation3 is the only gaming machine that delivers a complete high-definition entertainment system with a built-in Blu-ray player, hard drive, Wi-Fi, and full range multi-media capabilities.
|Country of Origin
|United States
Similar Items
Assassin's Creed The Ezio Collection - Playstation 4
Today: $56.74
Kingdom Hearts Hd 1.5 + 2.5 Remix - Playstation 4
Today: $56.18
Mass Effect Andromeda - Playstation 4
Today: $67.55
Warner Brothers - 1000507059 - Mortal Kombat X Ps4
Today: $27.19
Sony Playstation - 3001568 - Mlb The Show 17 Se Ps4
Today: $46.74
Diablo Iii - Playstation 3
Today: $20.37
Sony Playstation - 3001579 - Paragon Ps4
Today: $47.09
Product Reviews
Most Recent
PS3 Station
Bought this for my husband, and he loves it as do my sons. We love the fact that it is also a blue ray dvd player. It is slim and awesome to play.I just wish we had a option to pick what game it came with.Read More
Music Problem
Only issue we are having with the new PS3, when we are watching blu-ray/DVD movie's on it, the sound will cut out. We're not quite sure why. For some reason we have to disconnect the internet before we watch a movie to make sure the sound does not cut out while watching the movie, not sure what we can do to resolve the issue.Read More
Questions & Answers
Thank you for your inquiry; you will be contacted by Customer Care.
"Is the remote wireless in item:13883642"
Yes the controller is wireless. Please let us know if there is anything else we can do for you.
"Why does the sound cut in and out when playing, and it sometimes lags behind when playing"
That would be your internet connection. If your connected to the internet.
Shopping Tips & Inspiration
Sony PlayStation 4
Sony PlayStation 4 buying guide from Overstock.com. Check out the best features of the Sony PlayStation 4 and learn what games and accessories are a must have.
Video Game Consoles Buying Guide
Video Game Consoles Buying Guide from Overstock.com. Consider the features you want in a video game console, but your choice should be driven by choosing which games you are interested in playing.
Best Video Games for Christmas
Give the kid or budding gamer in your life what they really want for Christmas — a video game. This guide breaks down several game types, so you can choose the right game to slip into a stocking or put under the tree this year.
Video Game System Glossary
Video Game System Glossary from Overstock.com. If you're shopping for a video game console, you'll want to familiarize yourself with these terms.
Buying Gifts for Gamers
Buying Gifts for Gamers from Overstock.com. Our guides provide customers with information about buying gifts for gamers.
Television Checklist for Video Gaming
Television Checklist for Video Gaming from Overstock.com. Our guides provide customers with information about televisions for video gaming.
How to Clean Gaming Hardware and Accessories
How to Clean Gaming Hardware and Accessories from Overstock.com. Our guides provide customers with information about how to clean gaming hardware and accessories.
How to Choose a Video Game Console
How to Choose a Video Game Console from Overstock.com. Our guides provide customers with information about how to choose a video game console.
Best Hand-held Games
Best Hand-held Games from Overstock.com. It's time to go mobile! Here are some of the best portable video games to try.
10 Fun Facts about Disney
10 Fun Facts about Disney from Overstock.com. Disney has a long, exciting history, and here are some fun and lesser known facts.
Shipping & Returns
Contact Information
This product is not yet released, and is expected to ship on Nov. 1, 2011.
This date is subject to change. In order to assure you receive Overstock.com's low price on this item, your credit card will be charged upon order placement. The item will ship immediately upon release.
Standard Return Policy:
Items must be returned in new or unused condition and contain all original materials included with the shipment. More Details
FINAL SALE EXCLUSION: Items marked as FINAL SALE are not returnable unless the problem you experience is the result of our error.
For your protection, all orders are screened for security purposes. If your order is selected for review, our Loss Prevention Team may contact you by phone or email. There may be a two business day delay to process your order.
** Most Oversize orders are delivered within 1-4 weeks. Some orders may take 6 weeks to be delivered.


text:
Gaming Blog Blogs
A blog covering topics in the Roleplaying Game Industry with a focus on d20/D&D-clone products.
Owner: BA_Tracey
Listed in: Videogames
Other Tags: roleplaying games, Dungeons and Dragons, RPGs, Games, Board Games
A blog for everything Xbox 360 featuring honest reviews, previews and features along with all the.. latest news.
Owner: Ronie
Listed in: Videogames
This blog contains xbox 360 gaming related content such as gamerscore and achievement guides, game.. reviews, technical help with your console and games, reviews, recommendations, and much more.
Owner: noddegamra
Listed in: Videogames
Other Tags: xbox, 360, gamerscore, gaming, achievements
I am Fanscott this my gaming blog I help gamers out on Twitter or facebook and much more site I try.. to make them famous
Owner: Fanscott
Listed in: Videogames
Online gaming blog covering MMORPG's, FPS, and other games.
Owner: moku999
Listed in: Videogames
Other Tags: World of Warcraft, wow, mmorpg, mmo, Battlefield
We offer current news on both technology and gaming we get our information both first hand as well.. as from other sources around the net.
Owner: RNM1455
Listed in: Technology
Other Tags: Technology, Gaming, News
A digital culture blog, following upcoming games - and sometimes movies.
Owner: Elenandaron
Listed in: Videogames
Other Tags: Guild Wars, Blizzard, MMORPG, free to play, beta tests
Random Studios gaming is a blog founded by two immensely creative gamers with a mission- to tell the.. world what sucks, and what's worth your trip to Gamestop.
Owner: bobbob11235
Listed in: Entertainment
Other Tags: Gaming, Reviews, Satire, Technology, Video
9. Gaming Blog
Come here to learn all the latest gaming news and to find out how to get free Microsoft Points, Free.. 1600 Microsoft Points, Free 4000 Microsoft Points all e-mailed to you 100% free!
Owner: Baller95
Listed in: Videogames
Other Tags: games, xbox 360, free microsoft points, halo, cod
The Phantom Gaming Blog - Centered around video games, gaming, gamers, and gamer culture in general.
Owner: Phantom552
Listed in: Videogames
Other Tags: Game Guides, GameFAQs, MMORPG, FPS


text:
Boot Barn Item #: 2008007
Color: Click a button belowcolor
size: Click a button below Size Chartsize
- 95% polyester 5 % spandex
- Long 3/4 sleeves
- Stud detailing
- Boat neck neckline
- Knee length
- Hand wash cold
Dress for the occasion with this Studded Knit Dress by Roper. It features stud detailing that trails down the front of the dress with a fantastic boat neckline. Whether you are going to dinner or the dancehall, this is perfect for dress up occasions. Pair with cowgirl boots and a hat for a complete western outfit.
Style(s): 3-57-513-1182
Lifestyle: Western
Material: Polyester
Color: Black
Print Roper is America's authentic western wear. No imitations. You'll find superior design and quality. Hard work, vision and a dream. That's how the west was won. That's why Roper is America's first choice in authentic western wear and everyday casual fashion styles.


text:
GSA Visual Communication Graduate 2012
My house style is wonderfully bold and playful, and can be seen in these bright bright prints, badges and sketchbooks.
I am Glasgow based and work as the designer and director of Risotto Studio; Scotland's leading risograph print specialist.
Through commercial projects, artists' editions and my own work I promote the risograph process and fun graphic design!


text:
Banana Split Pudding Brownies
Recipe by BB2011
From Vegan With A Vengeance. This is a really fudgy brownie with a banana pudding on top that melts into the brownie layer and forms this unbelievable ooey gooey banana brownie concoction. Although you can hold it in your hand, you may want to serve it with a fork. Enjoy!
Top Review by magpie diner
Mmmm..watch out you may just eat the whole batch. This was a great thing to make with my over-ripe bananas! Emphasis on the "pudding" in the title, you couldn't exactly hold my batch in your hand, plate and fork were definitely required - but I like that. The puddingy-cakey part is so rich, and the top layer set really nicely. I wouldn't change a thing - except that I'd have some vanilla ice "cream" to go with it. I think I'll make this next time I have people over for dinner, because vegan or not, they're just GOOD!
See Discussion:1 Review
Ingredients Nutrition
- 113.39 g semisweet chocolate, chopped
- 236.59 ml very ripe banana, mashed (about 2 large)
- 78.07 ml canola oil
- 236.59 ml sugar
- 4.92 ml vanilla extract
- 177.44 ml all-purpose flour
- 59.14 ml cocoa powder
- 1.23 ml baking soda
- 0.59 ml salt
- 236.59 ml very ripe banana, mashed
- 29.58 ml sugar
- 59.14 ml soymilk
- 2.46 ml vanilla extract
- 14.79 ml arrowroot
Directions See How It's Made
- Preheat oven to 350°F
- Spray a 9x13-inch baking pan with nonstick cooking spray or very lightly grease with oil.
- TO MAKE THE BROWNIE BATTER: Melt the chocolate by placing in a pan or heat-proof mixing bowl over a small pot of boiling water.
- Stir with a heatproof spatula until completely melted, then remove from heat and set aside to cool.
- In a large mixing bowl, combine 1 cup of mashed banana, the oil, and the sugar. Use a handheld mixer to beat everything together for about a minute. If you don't have a mixer, use a strong fork and mix for about 3 minutes.
- Mix in the vanilla and melted chocolate.
- In a separate bowl, sift together the flour, cocoa powder, baking soda, and salt.
- Add this to the banana mixture in batches, mixing with the hand mixer as you go along. Mix for about 1 minute more.
- PREPARE THE BANANA TOPPING: In a small bowl, combine all topping ingredients and mix with the hand mixer (remember to rinse the beaters off first) for about a minute.
- TO ASSEMBLE AND BAKE THE BROWNIES: Spread the brownie batter evenly into baking pan. Pour the banana topping over that and spread evenly.
- Bake for 30 minutes. Remove from oven and let cool.
- After about 15 minutes, move it to the fridge until fully cooled.
- Cut into twelve squares, place a few slices of banana on top of each portion, and serve.


text:
Use your antibodies-online credentials, if available.
No Products on your Comparison List.
Your basket is empty.
Worldwide Shipping!
Find out more
Show all species
Show all synonyms
Select your species and application
anti-Mouse (Murine) PLCB3 Antibodies:
anti-Human PLCB3 Antibodies:
anti-Rat (Rattus) PLCB3 Antibodies:
Go to our pre-filtered search.
Human Polyclonal PLCB3 Primary Antibody for IHC, ELISA - ABIN1531468
Lagercrantz, Carson, Phelan, Grimmond, Rosén, Daré, Nordenskjöld, Hayward, Larsson, Weber: Genomic organization and complete cDNA sequence of the human phosphoinositide-specific phospholipase C beta 3 gene (PLCB3). in Genomics 1995
Human Polyclonal PLCB3 Primary Antibody for IHC, ELISA - ABIN1532482
Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. in Cell 2006
investigation of physiological involvement of PLCbeta3 signaling in ovulatory-size follicles: identification of PLCbeta3 as a mediator of LH-induced differentiation responses of granulosa cells; up-regulation of PLCbeta3 during oogenesis/ovulation
Gnb isoforms control a signaling pathway comprising Rac1, Plcbeta2, and Plcbeta3 leading to LFA-1 (show ITGAL Antibodies) activation and neutrophil arrest in vivo
Plcb3-deficient mice spontaneously develop atopic dermatitis-like skin lesions.
Data suggest that PLCbeta3 acts as a negative regulator of VEGF- (vascular endothelial growth factor A (show VEGFA Antibodies)-) mediated vascular hyperpermeability through intracellular calcium signaling.
GPCR (show GPBAR1 Antibodies) activation of Ras and PI3Kc in neutrophils depends on PLCb2 (show PLCb2 Antibodies)/b3 and the RasGEF RasGRP4 (show RASGRP4 Antibodies).
defined a PLC-beta3- and SHP-1-mediated signaling pathway for FcvarepsilonRI-mediated cytokine production
Cellular G(i)-G(q) synergism derives from direct supra-additive stimulation of phospholipase C (show PLC Antibodies)-beta3 by G protein subunits Gbetagamma and Galpha(q (show GNAQ Antibodies)).
Expression of Plcb3 was studied in a cell line during myoblast differentiation.
both PLCbeta2/beta3 and PI3Kgamma (show PIK3CG Antibodies) play vital roles in platelet cytoskeletal dynamics
results suggest that activation of PLC (show HSPG2 Antibodies)-b3 by pertussis toxin-sensitive G proteins is responsible for transient [Ca2 (show CA2 Antibodies)+]i increase in response to thrombin (show F2 Antibodies)
GPCR agonists ET-1, LPA, and thrombin activate PLCepsilon and PLCbeta3 in fibroblasts. Activation of these PLC isoforms displays agonist-specific temporal profiles; PLCbeta3 is predominantly involved in acute and PLCepsilon in sustained PI hydrolysis
We propose that unliganded PLC (show HSPG2 Antibodies)-beta exists in equilibrium between a closed conformation observed in crystal structures and an open conformation where the PH domain moves away from the EF hands. Therefore, intrinsic movement of the PH domain in PLC (show HSPG2 Antibodies)-beta modulates Gbetagamma access to its binding site.
the MCP1 (show CCL2 Antibodies)-induced cortactin (show CTTN Antibodies) phosphorylation is dependent on PLCb3-mediated PKC (show PRRT2 Antibodies) activation, and siRNA-mediated down-regulation of either of these molecules prevents cortactin (show CTTN Antibodies) interaction with WAVE2 (show WASF2 Antibodies)
membranes are integral for the activation of PLC (show HSPG2 Antibodies)-beta isozymes by diverse modulators.
the M3 muscarinic receptor (show CHRM3 Antibodies) maximizes the efficiency of PLCbeta3 signaling beyond its canonical role as a guanine nucleotide exchange factor (show RASGRF1 Antibodies) for Galpha (show SUCLG1 Antibodies).
This study provides an understanding of the structural basis for the PDZ (show INADL Antibodies)-mediated NHERF1 (show SLC9A3R1 Antibodies)-PLCbeta3 interaction that could prove valuable in selective drug design against CXCR2 (show CXCR2 Antibodies)-related cancers.
PLCbeta3 is enriched in the cytosol.
phospholipase C (show PLC Antibodies)-beta3 structure reveals the role of the its distal C-terminal domain
GPCR (show NMUR1 Antibodies) activation of Ras and PI3Kc in neutrophils depends on PLCb2 (show PLCb2 Antibodies)/b3 and the RasGEF RasGRP4 (show RASGRP4 Antibodies).
PDZ domain-containing 1 (PDZK1 (show PDZK1 Antibodies)) protein regulates phospholipase C (show PLC Antibodies)-beta3 (PLC (show HSPG2 Antibodies)-beta3)-specific activation of somatostatin (show SST Antibodies) by forming a ternary complex with PLC (show HSPG2 Antibodies)-beta3 and somatostatin (show SST Antibodies) receptors.
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
phospholipase C, beta 3 (phosphatidylinositol-specific)
, phospholipase C-beta-3
, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3
, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3-like
, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
, PLC-beta-3
, phosphoinositide phospholipase C-beta-3
, PLC beta 3
, phospholipase C beta 3


text:
Jury Selection Set to Begin in Child Abuse Death Case
Jury selection will begin in Adams County Monday for the trial of a man accused of beating his 2–year–old grandson to death last year in the South–Central Nebraska town of Naponee.
Peter Draper, 48, is charged with intentional child abuse resulting in Joseph Rinehart Junior's death. He died in April of 2012 after family members took him to the hospital for severe vomiting.
Court records say family members told police that they had watched Draper abuse the boy on several different occasions.
An autopsy showed Joseph died of head and internal injuries.
Draper is one of three family members charged in the case. His wife, 48-year-old Nancy Draper, and Joseph's mother, 25-year-old Laura Rinehart, are also charged with intentional child abuse resulting in death.
Nancy Draper's trial is set to begin June 17 in Phelps County. A trial date for Rinehart has not yet been set.
Defense attorneys for Peter Draper say his trial is expected to last five days.


text:
Direct advertisment created by M&C Saatchi, India for Dare, within the category: Media.
Copywriter: Sanjeev Saikia
Art Director: Keshav Hazra
Executive Creative Directors: Naren Kaimal, Amardeep Singh
Highest Rated
Very cool and strategic idea, but the art direction of the cards is completely uninspiring.
Saying 'done' without saying where, has been done to death.
We're going to need more lube.
One of the best comments I've ever read here. jajaja
Luis Maram
Hey, that's not a bad idea for a section actually, or a rating system. It would encourage people to be more creative with their posts.
We're going to need more lube.
It's not a bad idea. I think if the art direction of the cards was less slapstick then it would work well.
We're going to need more lube.
Where did they take the cards designs? A printing machine? Good idea, terrible execution.
Luis Maram
Nice idea... too bad that you can't influence the design of the cards ;)
love the idea...execution[business cards] lack aesthetics...but that doesn't undermine the power of the brilliant idea
Popular Reads
Clio Sports
"Say Less, Do More": Words of Inspiration From the 2017 Clio Sports AwardsRead More
Inside the Agency
How DDB's Ari Weiss And Keith Reinhard Are Embracing Ad Legend Bill Bernbach's "Rebel Spirit"Read More
Clio Health
Ward+Robes Designer Gowns Offer Individuality for Hospitalized TeensRead More
Clio Awards
Empathy, Observation, Openness: IDEO's Roshi Givechi on the Narrative of Product DesignRead More


text:
Speaking to Ecuadorian Gama TV, Chamorro claimed Benitez, known as "Chucho", had suffered a heart-attack. He had complained of abdominal pains and was transferred to a hospital, where he died on Monday, Chamorro said.
Benitez, who was top scorer in the Mexican league last season with Club America, had just signed for Qatari outfit El-Jaish and had travelled to the club to undergo a medical before completing his move.


text:
|Property type
|Minimum Stay
|2 - 3 nights
This Awesome Family Farm House is fully furnished with 5 Bedrooms, a Large Kitchen, a Huge back deck with a Grill, Bon Fire Area, and Plenty of Parking for All your Toys! It is also equiped with a Washer & Dryer, & Dishwasher.
With your stay you will also receive 4-9 hole rounds of golf at Golden Sands Golf & Bucket Bar for Each Night you stay for FREE!
It is located on the #1st hole of Golden Sands Golf Course, close to the Club House. Set just 2 miles from the Silver Lake Sands Dunes, and close to Pentwater, Ludington, and Muskegon you would be able to visit all of West Michigan without having to repack your bags! You also have tons of private parking for you and your family so bring all the toys with you! Oceana County is definatly Michigan's play ground with the Dunes, Dirt Biking, Hiking, Water, Racing, Fishing, Lighthouses, and SO MUCH MORE!
There is also Silver Lake, Pentwater Lake, and Lake Mi. boating available near by! Boating is unbelievable up here! 2 Lighthouses, miles of private beaches, and even a Sunken Ship you can see from your boat!
Only 20 minutes away is Michigan's Adventure! This awesome theme park full of Water, Coasters, and Fun!
Main Floor
Beds All beds have 1 set of sheets & 1 pillow for each head
Laundry Room: Full sized washer & dryer
Living Room: Full sized couch & 2 chairs, Direct TV satellite with a flat screen (with Tigers & Sports games!)
Dining Room: Round table with four chairs, this space extends from the Living Room
Grandma's room: Full bed, small room with 1 small closet across from bathroom
Family bedroom: King Bed, couch, dresser, Lots of room for your air mattress or pack & play. Air Conditioning Window Unit
Full Bathroom: Full bathtub with shower, Pull Knob towards you to turn on shower. No bath towels (Please bring your own towels)
Kitchen: Stove, oven, full sized refrigerator, dishwasher, coffee maker, toaster, dishes, glasses, coffee cups, silverware, kitchen towels, dining table with 4 chairs.
Full Bathroom: Full bathtub with shower. No bath towels (Please bring your own)
Master Bedroom: King sized bed, dresser, room for your air mattresses, lots of closet space, window air conditioner
Adjoining Rooms: One Full beds, Three Twins beds, closet space, and a beautiful elevated outdoor deck
Back Yard: Propane grill (bring your own propane), large deck, bon fire pit, picnic table, 1/2 fenced in yard
Bon Fire Wood: $20 If you would like a big box filled with dry firewood a 24 hour notice is required. This is an outside company and price and delivery depends on availability.
Please think of this Cottage as your Grandma's home. If you need something, use something. If you have something leave something. (Like extra laundry soap :) The next closest house is almost a mile away. The Pro Shop & Restaurant is just steps away. There is a very large pond a short walk away. There is a lot of room in the front of the house and side of the house for parking large vehicles. The entire house is surrounded by big Maple trees that keep the house cool in the summer.
There is no central air conditioning, but there is 2 window air conditioning units. In Silver Lake there is usually is a strong breeze coming from Lake Mi. That's because the Silver Lake Area is set 2 miles out into Lake Michigan.! So remember to bring an extra sweatshirt. There is a few window fans in the house to help circulate air when it does get warm. Place these in the windows to bring the cooler night air in.
There is not beach towels, or bath towels supplied. There is kitchen towels. There is a private paved drive for you on the south side of the house. Please use these spaces for your family. Any repairs to vehicles must be on this pavement. The back yard & front yard is the best places for children to play. Please use golf etiquette with noise for the happiness of all guests at Golden Sands. (Revving engines lots & loud music is not good etiquette :)
When golfing & dining, all alcoholic beverages must be purchased at Golden Sands.
Your Free Golf may be used all at once, or spread out during your stay. There is a driving range, practice putting green, as well as the 9 hole golf course. Golf carts are available to be used on the golf course for an additional fee.
Please place all dirty sheets by the washing machine before check out. Clean out refrigerator, place garbage in the garbage bins, run dishwasher for dirty dishes.
Check in time is 3:00pm Check out is 11:00am
DIRECTIONS TIPS: We are set 2 miles from Lk. Michigan & Silver Lake Sand Dunes. When entering our address, DO NOT put NORTH wilson rd. It will lead you astray. Please use 2501 Wilson Rd. Mears, Mi. 49436. Silver Lake is usually called Mears on all mailings & GPS.
Golden Sands is ranked #1 on Trip Advisor! Silver Lake Sand Dunes has been nominated 'One of America's Best Little Beach towns' by Travel & Leisure Magazine!
Check out www.thinkdunes.com for lots to do while you are here!
Anyway you Slice it –
Your vacation will be filled with Awesome Memories that will Last a Lifetime!
Thank You for Choosing Golden Sands Cottage on the Course
Keywords: Family Home, House, Golf Course Views, Silver Lake Sand Dunes area, Children Friendly, large back yard, bon fire pit, large kitchen, lots of parking, Located on Golden Sands Golf Course and Bucket Bar Properties.
My husband and I own and run Golden Sands Golf Course. We used to live in the Golf House until 2008. We have 4 children and dog, Mulligan. When you stay with us you get your very own tour guides for the area! We love to travel all over our county and play! We can tell you which beaches on which days, which courses to play, and which restaurants have the best service! We are excited to help you make your vacation as memorable as possible!
Alicia Kolenda purchased this farmhouse in 1999
Silver Lake Sand Dunes is SO Breathtaking! The miles of sand, the fresh water, and the abundent ways to enjoy nature!
Free Golf - Free Golf - Free Golf ;-)
Add in the relaxation of no other houses within almost a 1/2 mile, Tons of wildlife to watch, and a restaurant on demand if you get sick of cooking! Perfect!
You could literaly vacation in Ludington, Pentwater, Silver Lake, AND Muskegon, without having to pack up and move! Play for the day - then come back to the Golf House to stay! SO Convenient!
Nice house plenty of room for everyone and lots for kids to do
Great area, great experience
The house was very roomy. We had 3 adults and 6 kids, and there was plenty of room for everyone. The kids loved running around the large backyard and the pond. The beds were comfortable and clean. The restaurant/bar is steps away and had games for everyone to borrow and play. It was a great family vacation and we all had a wonderful time. FYI the cell service is challenging, but it kept the kids off the electronics and made them go outside and play. There is free wifi.
Most people come here for the Dunes (about a mile away) or the multiple golf courses in the area, but for us, it served as home base for our annual disc golf weekend. Eight guys ranging in age from 29-50 and we all had a blast. The house was clean and had everything we needed to survive the weekend without our wives (dishes, cookware, washer/dryer, etc.). Each guy had a comfortable bed to sleep on each night. We had our own driveway for our vehicles that led to the back porch area. The backyard had a deck with a grill, a fire pit and plenty of space to hang out. The house had a balcony that overlooked the property's beautiful ball golf course complete with a pond, driving range, pro shop and Bucket Bar. The Bucket Bar is located just steps from the backyard area of the house and serves some of the best pizza, wings and sandwiches we have ever had (a good thing because its one of the only restaurants in the area). The restaurant was very popular with the locals and had a family atmosphere. The wonderful owners of the property did everything they could make sure our stay was perfect. After taking care of the golf course during the day, they run the Bucket Bar at night. Alicia was so sweet, funny and down to earth, plus she makes a mean long island iced tea. We also had cleaning supplies to wash our dirty clothes thanks to Alicia's concept of treating the house like "Grandma's House" (use what you need and if you brought supplies feel free to leave extras behind for the next group). I really can't say enough good things about our stay here. Some other reviews mentioned cosmetic flaws in the house but you can't compare a hotel room to an entire house built in the 1800s, especially for a fraction of the cost. This isn't a commercially ran property. Its a good family house run by an even better family. We will definitely be returning for future outings!
Worked perfect for our group of seven people. Accurate description and very helpful owners. This place is walking distance from the bar! Highly recommend!
Disappointed. Carpet had big stains, grill was disgusting. no working fans. Fuses blew when you plugged toaster and coffee pot in .House had flies in it all the time bad screens or no screens on some windows. Large deck on back of house but side of house was so dirty and covered with spider webs and spiders. Many broken items in home not well maintained musky smell. Many other issues definitely not worth what we paid .
We are located just 2 miles from downtown Silver Lake with lots of family friendly activities. From Mac Woods Dune rides, to Craigs Cruisers amusements. There is lots of activities to keep you active!
|Rate Period
|Weekend Night
|Monthly *
Summer Rates
Jun 23 2017 - Sep 4 2017
3 night minimum stay
Fall Rates
Sep 5 2017 - Oct 31 2017
2 night minimum stay
My Standard Rate
2 night minimum stay
Once our guests have confirmed their available dates, the $100 deposit & booking fee may be paid in many ways. You can either mail a check, call with a credit card, or go to our website and pay online. www.GoldenSandsGolfCourse.com click the Tee Time & Shop red tab at the top of the page. Look for the Deposits section. Full balance is due upon check in.
* Approximate monthly rate. Actual rate will depend on the days of the month you stay.


text:
The rapid collapse of a U.S. diplomatic compound in Libya exposed the vulnerabilities of State Department facilities overseas. But the CIA ability to fend off a second attack that same night provided a glimpse of a key element in the agency's defensive arsenal: a secret security force created after the Sept. 11, 2001, attacks.
Read Full Article »


text:
Hoyer Announces Winners of 2014 Member Online All-Star Competition
July 21, 2014
Mariel Saez 202-225-3130
WASHINGTON, DC - House Democratic Whip Steny H. Hoyer (MD) announced the winners of the 2014 Member Online All-Star Competition today. See below for the list of Members, Committees, and Caucuses that have been named 2014 Online All-Stars.
"I'm pleased to announce this year's Online All-Stars, who led House Democrats in acquiring over 213,000 new followers on Facebook, Twitter, Instagram, and Youtube," stated Whip Hoyer. "Over this three-week competition, House Democrats did an outstanding job connecting with hundreds of thousands of new followers, surpassing last year's total by 50,000. I congratulate Reps. John Lewis, Joaquin Castro, and Dan Kildee on being named MVPs, and I thank all of the Members and their staffs for their hard work and innovative efforts to reach and engage with more Americans through social media."
The 2014 MVP, Rep. John Lewis (GA-05), gained an impressive 17,495 new followers, thanks to his engaging content, including photos from his work in the civil rights movement in the 1960s, which he compared to today's work for LGBT rights, as well as posts on immigration reform. Freshman MVP Joaquin Castro (TX-20) gained 6,051 new followers in part by hosting a Facebook Q&A virtual townhall, hosting a constituent cover photo contest, and bringing attention to the humanitarian crisis at the border. Overall, House Democratic Freshmen did an extraordinary job competing in the contest, holding 15 of the top 50 All-Star spots.
Out of the 58 House Democrats who were in a pre-election Franking blackout period, which prohibits some actions like advertisements, Rep. Dan Kildee (MI-05) won the Election Blackout MVP award by gaining 2,873 new followers, in part by pushing to renew unemployment benefits and posting a "selfie" with fellow Reps. Takano and Lowenthal.
Committee Democrats and Caucuses also participated in the competition. The Congressional Black Caucus, the Congressional Hispanic Caucus, Oversight & Government Reform Committee Democrats, Ways & Means Committee Democrats, and Science, Space, & Technology Committee Democrats were named 2014 All-Stars.
This year's total of over 213,000 new followers for House Democrats on social networks marks an impressive increase over the previous year's total:
Total new
MVP Rep. John Lewis (GA-05), Freshman MVP Rep. Joaquin Castro (TX-20), and Election Blackout MVP Rep. Dan Kildee (MI-05)
MVP Rep. Luis Gutierrez (IL-04) and Freshman MVP Rep. Mark Takano (CA-41)
MVP Rep. Jared Polis (CO-02)
MVP Rep. Maxine Waters (CA-35)
Co-MVP Rep. Pedro Pierluisi (PR) and Co-MVP Rep. Jared Polis (CO-02)
This year's competition included Instagram for the first time, and the contest resulted in the near doubling of House Democrats with Instagram accounts, from 50 accounts prior to the competition to 88 accounts now. House Democrats gained 6,500 new followers on Instagram during the competition.
Note: Members of Leadership, while participating in the competition, were not counted in determining All-Stars and are not included in the list below.
2014 Member All-Stars:
2014 Committee All-Stars:
2014 Caucus All-Stars:


text:
What is your username? Maximagination
Please link to the wiki here: http://yogabbagabba.wikia.com/wiki/Yo_Gabba_Gabba_Wiki
How many edits have you made on that wiki? 13 edits.
How many days/months have you been editing there? Since March 16th.
On the Special Pages -> Special:ListUsers when was the last time an admin edited, and who was it? I can't find the admins.
Adding the info for you - May 9th Maximagination 3 admins in total have logged in on May 9th (even though only one edited) Cheers Dawn
Any other information: This forum has been abandoned. Edits are rare, and when edits are done, they are not appropriate. I don't know too much about being an admin on a wikia, but I will do all I can for this. Please make me admin, for I will help fix up this wiki! :)
- Thank you so much! [http:// http://community.wikia.com/wiki/User:Maximagination]


text:
The aftermarket version of General Motors' in-car connectivity system, OnStar, is starting to pay off for drivers of non-GM vehicles.
At least that was the case Dec. 18 when a Gresham, Ore., owner of a Chrysler 300 was able to recover her stolen car with the aid of the OnStar system, which police used to track it down.
OnStar is that one-button system that allows you to talk to a live operator. It was initially introduced as a built-in in all GM vehicles so owners could reach an operator in case of an emergency. GM has steadily added more features to it, such as turn-by-turn navigation.
In the latest twist, GM has introduced an OnStar system that can be retrofitted to cars from rival brands. It's as easy as replacing the rear-view mirror. GM says OnStar plays a role in recovering 500 stolen cars a month. Now, it even allowed for a recovery of a competitive brand's car.
OnStar subscriber Jean Franklin of Gresham, bought the OnStar system two days before her 2006 Chrysler was stolen. It came with a stolen vehicle recovery feature.
"The stolen vehicle feature is the reason I purchased [OnStar FMV]," Franklin told GM. "My car had been stolen before, so the police recommended I get it. I am much more comfortable now that the car has OnStar."
GM says Franklin called OnStar to report her stolen vehicle around 7:30 a.m. on Sunday. After OnStar provided the Gresham Police Department with the vehicle's location, officers were able to pull over the car and had the suspect in custody within 64 minutes of the original call. GM created a fake version of the incident for the photo, above, which it calls a dramatization.
"OnStar was crucial to recovering the vehicle," said Aaron Turnage, a Gresham Police Department officer who worked the case tells GM. "Without OnStar, we probably would have recovered it, but who knows what condition it may have been in? OnStar helped get the vehicle recovered almost immediately, and it was completely unharmed."
Chris Woodyard is an auto writer for USA TODAY who covers all aspects of motoring. He revels in the exhaust note of a Maserati and the sharp creases of a Cadillac CTS. Chris strives to live a Porsche life on a Scion budget. More about Chris


text:
(if this is a private computer)
Yes please, register now!
Start a Conversation
Add A Photo
When you purchase a digital subscription to Cake Central Magazine, you will get an instant and automatic download of the most recent issue.
storybook doll pan. Made for my 5 yr old.
Pink/black Ballerina Cake
Delphina's Fairy Ballerina
Barbie Doll
Too cute!! Great job!
i love the different textures. very pretty!
Nice job!
What a sweet one.
Awesome job! Pretty cake!


text:
I am happy to announce the release of wallet version 0.9.0.2 for Windows, OS X and GNU/Linux. This is a bug fix release which aims to fix the vast majority of problems with the current version. If you have problems please don't hesitate to create a new issue on GitHub so we can incorporate a fix into the next version (likely 0.9.0.3). A wallet update is not required but is strongly advised to ensure you are on the most stable wallet build.
Downloads and bug fix list:


text:
uae jobs chanel beige perfume 00 to qualify GOLD Offers 8% off valentino couture dresses all orders* Spend £.
00 to qualify PLATINUM Offers 10% off all orders* Spend £.
Privacy Policy Website Copyright ©.
00 Customize now Custom Fast Jacket&trade.
ve each bagged two of the most eligible bachelors in the world, proving something we'.
The Cheapest chanel beige perfume Cheap Original Sale Paraguay chanel beige perfume knight51 by Meduza 2 hours ago 2 valentino rockstud collection hours ago Replies: 1 Views: 81 1 Nautical Science OR Navigation and Maritime science.
PERSONAL STATEMENT by Moking93 by Meduza 2 hours ago 2 hours ago Replies: 1 Views: 83 1 Unplanned gap year personal statement.
by theformula by Meduza 2 hours ago 2 hours ago Replies: 1 Views: 80 2199 Track checkers not so anonymous.
) by Coffeetime by Coffeetime 3 hours ago 3 hours ago Replies: 2,199 Views: 123,525 607 Any one resitting A2 Modules.
) by TheGemini by Teddysmith123 3 hours ago 3 hours ago Replies: 607 Views: 19,410 Moved: Architecture 2015.
Started by: Captain Jack Forum: Food and drink Replies: 0 Last post: 3 minutes ago I did a masters at QMUL.
Started by: Zamestaneh Forum: Religion Replies: 412 Last post: 6 minutes ago Racist Stereotypes, Are You a Victim.
Started by: Rosie786 Forum: Forum games Replies: 2586 Last post: 5 minutes ago See coach outlet camarillo more Study resources "Justice should not only be done, but should manifestly and undoubtedly be seen to be done.
" Lord Hewart AQA Psychology Unit 1 Social Learning Theory National Curriculum requirements chapter 7.
Copyright The Student Room 2014 all rights reserved The Student Room, Get Revising and Marked by Teachers are trading names of The Student Room Group Ltd. Stylish And High Quality Authentic Luxury Items Outlet Online, chanel beige perfume order more than 200usa get big discount on here.
This website contains sexually explicit content.
You new bottega veneta bags handbags must be at least 18 years of age to enter this website.
Please enter a title Please enter a message Post Close x Results are out.
get Clearing help Get quick advise or join the chat celebrate your results Make a speed post: ask a question about Clearing Please enter a title Please enter a message Post Looking for something else.
Try the All Forums page Close x Thanks for posting. chanel beige perfume RD
Supply cheap and wholesale chanel beige perfume order online or direct from our store. uae jobs chanel beige perfume 00 Customize now Custom Racing Jacket®.
s brains not beauty that puts a ring new bottega veneta bags 2014 on it Sophie Turner, Benedict Cumberbatch's new fiancee Rex 1.
00 Customize now Custom Radarlock&trade.
s bilingual, having studied French and Italian at Oxford University and later at the esteemed Jacques Loco School of theatre in Paris.
00 Customize now Custom M Frame®. chanel bedding set for \u002420.00chanel beige perfume costchanel beige perfume reviewschanel beauty associatechanel beige clair wocchanel bangles pricechanel bedding fullchanel beauty gift with purchasechanel beaded handbagchanel beige perfume


text:
July 23, 2016
dedicated short code, marketing campaign, marketing strategy, mobile marketing, mobile platform
How To Increase Profits Through Mobile Marketing
Have you heard about mobile marketing? There are a zillion different ways to use mobile technology to market products and services. Wondering where to begin in such a broad topic? Follow these tips to get started. You must have a proper database built to start a mobile marketing campaign. Refrain mindlessly adding phone numbers in […]


text:
Click here to print this page
Gibbs Charitable Trust
Mrs. L. A. Nelson
Mr. & Mrs. D. Naish
Mrs. J. M. Angerson
Burges Salmon Charitable Trust
Mrs. M. E. Dyer
Made for Ever Wives Group
Mr. & Mrs. P. J. Revill
Mrs. R. C. Vincent
Margaret Heard
Mr. & Mrs. G. Barnes
Mr. H. J. Hibberd
Mrs. M. Spivey
Mr. & Mrs. M. Yates
Mr. & Mrs. G. Stock
Mr. & Mrs. A. Hudson
Mrs. Penny Moon
Professor R. Hodder-Williams
Mrs. M. Fisher
Mrs. Vivien Evans
Mrs. M. Press
Mrs. M. Bryant
Mrs. J. H. Gibbs
Mr. R. V. Davies
R. Burgess
Mr. & Mrs. D. M. W. Simpson
Mr. B. Trowbridge
Mr. J. E. Barnett
Rev. E. A. & Mrs. Clarke
Miss H. J. Cross
Mrs.J. Verinder
R. N. Goldsworthy
Mr D. & Mrs. P. J. Blackmore
Mr & Mrs J.D. Wisheart
Staple Hill Methodist Church Ladies Group
T.A Scadding
J. Taylor
J.E. Brown
D. Bainbridge
D. Smart
West Town Methodist Church
Mrs. K M. Pillinger
Elizabeth Radendge
Watley's End Methodist Church
Beryl Bellamy
J & M Britton Charitable Trust
Rev & Mrs D Weeks
Stapleton Methodist Church
The Merchant Venturers
The Misses Buston
Scobell Charitable Trust
Hanham Methodist Church
Dame Violet Wills Will Trust
Mr B Glasson & Ms L Robinson
Mrs D M Symons on behalf of the Montacute family
Mrs S Howdle
Westbury Methodist Church Wesley Guild
Revd. Lee, Cheon Sik and the Methodist Church of Korea
Mrs B Pidgen
Mrs B Hickling
Ashridge Construction Ltd.
Miss M K Croft
Filton Methodist Church Wives Group
The Leonard Laity Stoate Charitable Trust,
Hartnett Conservation Trust
Wesley Historical Society, Bristol Branch
Allchurches Trust
Westbury Methodist Women's Fellowship
The four stone inscriptions around the base of the John Wesley statue were unveiled by The Lord Mayor of Bristol Geoffrey Gollop, Revd Dr Mark Wakelin from the Connexional Team of the Methodist Church, Revd Ward Jones Chairman of the Bristol Methodist District and Chairman of the New Room Trustees and Revd David Weeks Chaplain and former Warden of the New Room.
The event was also attended by the BBC Points West news programme and their weatherman, Richard Angwin, who presented his forecast from the courtyard garden.
There is a photo diary on the New Room Facebook page which show the garden being created. Please click on the link on the home page of this website to view this. Regular updates will also continue to be posted as the garden grows!


text:
Navy Health Optical Rebates
Adel Eyecare offers Navy Health members the opportunity to maximise the benefits of private health insurance.
Adel Eyecare offers Navy Health members:
- A 'No Gap' range – a special for Navy Health members to purchase our fantastic frame and lens packages at no out-of-pocked expense
- Access to Adel Eyecare's eye testing and consultation services – in depth assessment workplace/office needs and for ocular disease prevention including diabetes, macular degeneration and glaucoma. Ask about Medicare bulk-billing.
- A full 2 year satisfaction guarantee on all Packages
- The lowest price sunglasses and prescriptions sunglasses guaranteed
Details of your Navy Health membership type should be on the front of your card. Adel Eyecare does not guarantee the information below as it can change at any time, information is provided as accurately as possible as a guide. Navy Health's extra packages and the benefit limits for optical are:
|Premium Extras
|Healthy Living
|Saver Plus
|Basic Extras
|Annual Limit
Item numbers for optical health fund rebates include:
|Single Vision Lenses
|Bifocals Lenses
|Multifocal Lenses
|Contact Lenses (frequent replacement)
A full list of item numbers for optical services can be found through HICAPS by clicking here.


text:
Ripped Femme Metabolism Support
Designed Specifically for the Female Hormonal System
When it comes to burning fat, most fat burners forget that a woman's metabolism and hormonal system is different than a man's. A woman's unique biochemistry is a balance of multiple hormones that affect her energy, workout performance and ability to burn off fat. Ripped Femme METABOLISM SUPPORT is the first fat burner supplement that's truly formulated for a woman's unique endocrine system. It delivers clinically researched dosages of key fat-burning ingredients, including indol-3-carbinol, red pepper extract, green tea extract, caffeine, olive leaf extract, vitamin B12, and a supportive dose of L-carnitine tartrate.
What Makes METABOLISM SUPPORT Unique?
METABOLISM SUPPORT is formulated with two unique complexes that work to support optimal female fat metabolism. Unlike other fat burners that only solve part of the problem, METABOLISM SUPPORT works to balance out the female hormonal system, unlocking estrogen's hold on your fat-burning potential and allowing you to burn off more fat.
Estrogen Metabolism Complex:
Fat Metabolism Complex:
How does METABOLISM SUPPORT Work?
METABOLISM SUPPORT works by promoting estrogen metabolism and a healthy balance of estrogen in the body, while supporting fat metabolism through increased thermogenesis. When estrogen levels are high, the result is metabolic shutdown. Excess estrogen blocks fat-burning receptors from activating fat metabolism and the burning of fat as fuel. METABOLISM SUPPORT delivers key ingredients to lower estrogen load and ignite fat metabolism via multiple pathways!
Estrogen & Fat Metabolizer Complex:
Indol-3-carbinol is a unique compound isolated from cruciferous vegetables such as broccoli, and it has been shown to have a positive effect on estrogen metabolism, neutralizing harmful estrogen metabolites and balancing the ratio of estrogen in the body.
Fat Metabolism Complex:
Sweet Red Pepper Extract
Sweet red pepper extract is a non-pungent (non-spicy) cultivar of red pepper and contains three known capsaicinoid-like substances that can boost fat burning: capsiate, dihydrocapsiate and nordihydrocapsiate. Sweet red pepper extract can enhance core body temperature, oxygen consumption, uncoupling proteins 1 and 2 (UCP1 and UCP2) and even gene transcription of UCP2 mRNA in fat tissue!
Caffeine has a synergistic effect when combined with other fat-burning ingredients. It blocks the action of phosphodiesterase (PDE), an enzyme responsible for the breakdown of cyclic adenosine monophosphate (cAMP). Stopping the breakdown of cAMP results in a prolonged effect of cAMP-dependent pathways, which include regulation of glycogen, blood sugars and fat metabolism. Further to that, prolonging cAMP's actions increases the release and mobilization of fat from fat cells, allowing them to be burned off during exercise or even while at rest. Increased cAMP activity also results in greater release and availability of norepinephrine, one of the key hormones involved in fat burning!
L-Carnitine L-Tartrate
Carnitine plays a key role in fat metabolism in the body and helps shuttle fat to the powerhouse mitochondria to be burned off as fuel. Carnitine levels are lower in women; therefore, increasing carnitine via supplementation can help increase carnitine levels and support fat metabolism in the female body.
Green Tea Extract
Green tea extract has a twofold effect when it comes to female fat burning. The active compound, EGCG, can help stimulate thermogenesis and norepinephrine release. Green tea extract can also help decrease the aromatization of "bad" estrogen. High levels of estrogen can block fat burning in the body, but green tea's positive effects can help lower estrogen load and elevate fat burning.
Olive Leaf Extract
Olive leaf extract provides the active compound oleuropein, which has a direct effect on UCPs, upregulating its effects in brown adipose tissue and increasing the expression of the fat-burning hormones norepinephrine and epinephrine.
Vitamin B12
Vitamin B12 is an important energizing vitamin, which helps the body metabolize carbohydrates and fats. Vitamin B12 is also involved in the production of red blood cells, which carry oxygen to muscles during workouts. Low levels of vitamin B12 have been associated with hormone imbalances in the body, not to mention fatigue and depressed mood.
RECOMMENDED USE: Take 1 capsule 3 times daily, every 3 to 4 hours as needed. Take with food. For occasional use only. Consult a health care practitioner for use beyond 12 weeks.
Supplements Canada, or the operating company, will not be held responsible for any product information and ingredients,


text:
Need Food Allergy Testing in Marshall, TX? Simply order online 24/7 and get fast, affordable Food Allergy Testing at a lab near you (Find More Locations):
Why would I get Food Allergy Testing?
Food Allergy Testing is useful for people in Marshall, TX who suffer from symptoms such as unexplained rashes, bloating, itching or runny noses that may be caused by exposure to foods.
How much does Food Allergy Testing cost?
Our Food Allergy Testing prices include the doctor's order that you need to get tested in Marshall, TX, all lab fees and taxes, and a pdf copy of your results. For current prices, please CLICK HERE.
We are pleased to offer Food Allergy Testing in Marshall, TX.
Food Allergy Testing in other cities near Marshall, TX:Beckville | Easton | Elysian Fields | Hallsville | Harleton | Jefferson | Jonesville | Karnack | Lodi | Longview | Scottsville | Tatum | Waskom | Woodlawn


text:
Find used dimmer modules by top manufacturers such as Applied Electronics, Avolites, NSI and Leprecon. All products come with warranties and are tested before shipping to you.
Solaris sells and buys secondhand and used dimmer modules for concerts, installations, churches, events and all types of entertainment production. If you do not find what you are looking for give us a call and we will quickly find it for you.


text:
Muffy VanderBear Lulu Lot
- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: -
- Source: eBay
- Sold Date: Dec 14, 2012
- Channel: Auction House Sell similar item on eBay
Muffy VanderBear Lulu Lot. Includes Lulu with original hang tag Lulu's dog bed, leash, dog bowl and brush in unopened package. From the introduction of Lulu in Fall 1993. All with original hang tags . From a smoke free home. No returns. No International Shipping. Paypal only. Excellecnt Condition.


text:
All Android applications categories
Mp3 Music Search Download Pro allow you download mp3 music through the best music search engine.
Search and download mp3 music from public domain via public search engines.
After download, you use your default music player to play the song! You don't have to move the songs! Only try to refresh your music library!
Not invasive like all the others! NO PUSH NOTIFICATION ADS!
Tested on: Galaxy Nexus, SGS2, SGSIII, Nexus 7, Nexus S, Desire HD, Desire S, Optimus 3D, Razr, Asus Transformer Prime, Xoom and various TEGRA 2-3 devices..
from 959 reviews
Download Mp3 Search and Download Pro
Free - V1.0 - 2M
Sorry ...
This app is no longer available.
Share this app


text:
Travel and Expense Processor
- Employer
- Plus Relocation Services
- Location
- Minneapolis, Minnesota
- Posted
- Dec 14, 2016
- Closes
- Jan 13, 2017
- Category
- Accounting, Administrative / Clerical, Banking, Business, Career Fairs, Customer Service, Financial Services, Government, Nonprofit, Other, QA / Quality Control, Real Estate
- Job Type
- Employee
- Employment Status
- Full Time
Plus is driven to be the best relocation management company in the industry. We are committed to providing completely customized global mobility programs and flawless execution. Our size is just right – not so vast that you become a number and not so small that any possibility is too great. We invest in our employees by concentrating on continuous training, personal development, and utilizing the Gallup Strengths Finder to explore individual strengths. We understand how important a work-life balance is and believe in creating opportunities to grow both professionally and personally.
We are headquartered in Minneapolis, MN United States with additional offices in London, Hong Kong, and soon to be Shanghai, however we certainly do not believe our global expansion stops there. Our tri-regional model allows us to deliver seamless mobility services in more than 180 countries – and counting!
Essential Job Functions
- Review relocating employee expenses for accuracy, compliance, expense coding and policy adherence in a timely manner
- Review supplier invoices for accuracy, compliance, expense coding and policy adherence in a timely manner
- Accurately calculate and provide timely tracking of supplier revenue
- Generate precise and timely client invoices
- Report on supplier aged invoice status
- Communicate with internal customers and supplier partners to resolve billing issues, expense issues and general questions
- Generate internal and external reporting as defined by management
- Assist other departments as necessary
- Coordinate the processing of client candidate expense reimbursements
Skills / Abilities
- Strong organizational skills with the ability to multitask at a high level
- Capable of working effectively and independently in a fast-paced environment
- Ability to be a self-starter and work autonomously, as well as be a part of a successful team
- Proficient in utilizing Microsoft Excel
- Excellent numerical and analytical skills
- College degree preferred
- Minimum of 1 year of accounting, employee expense management or related experience preferred
- Advanced knowledge of Excel functions and formulas
Why choose Plus Relocation?
Our vision, mission, and values says it all - we have the best employees who care and want to make a difference. Below are just a few additional perks of working at Plus:
- Use of Gallup Strengths Finder
- Work/Life Balance
- Comprehensive health package
- 401(k) plan
- Profit Sharing
- On-Site Gym Membership ($10 annually)
- On-Site Yoga
- Free Personal Training (remote or at on-site gym)
- Close to downtown Minneapolis (394/169)
- Free Ramp Parking
- Wellbeing Program
- Leadership Focused Development
Want to learn more? Check us out at http://www.plusrelocation.com/careers/. We look forward to hearing from you!


text:
VCF West is for everyone! Computer geeks, families/children, students, collectors, IT professionals, curious onlookers…
When: August 5-6, 2017
With support from:
Where: Computer History Museum, 1401 N. Shoreline Blvd., Mountain View, California (Google map, directions, lodging)
Hours: Saturday, 9:00-6:00, Sunday, 9:00-5:30
Tickets — Available online and at the door.
Exhibits (and exhibit registration) — Hands-on exhibits are presented Saturday and Sunday. You'll find demos of 1960s minicomputers, 1970s homebrew systems, 1980s eight-bitters, and a few oddities. Some exhibits contain pristine original machines, while others focus on unique modern hacks, and everything in between.
Speakers — Come see our impressive keynote speakers!
Consignment — Vintage Computer Festivals are not flea/swap events, however we do offer a consignment room. Note: We're making a new consignment form. It will be available shortly.
Food — Where they are computer nerds, there must be food! Items such as bagels, muffins, coffee, snacks, hot dogs, soft drinks, etc. will be available.
Don't forget — CHM is the world's best computer museum, and it will be fully open during VCF West. Do not miss your chance to see their incredible exhibits including the newly constructed software pavilion.
Contacts: VCF West Producer — Erik Klein (firstname.lastname@example.com), Vintage Computer Federation President — Evan Koblentz (email@example.com)