text:
Identification of drought-responsive and novel
Plant genomics
MicroRNAs (miRNAs) are endogenous small RNAs (sRNAs) with a wide range of regulatory functions in plant development and stress responses. Although miRNAs associated with plant drought stress tolerance have been studied, the use of high-throughput sequencing can provide a much deeper understanding of miRNAs. Drought is a common stress that limits the growth of plants. To obtain more insight into the role of miRNAs in drought stress, Illumina sequencing of
Two sRNA libraries were constructed by sequencing data of control and drought stress treatments of poplar leaves. In total, 207
We discovered five upregulated miRNAs and seven downregulated miRNAs in response to drought stress. A total of 72 related target genes were detected by degradome sequencing. These findings reveal important information about the regulation mechanism of miRNAs in
MicroRNAs (miRNAs) are one of the most abundant classes of small RNAs (sRNAs) in plants and animals. These endogenous sRNAs were first identified in a metazoan called
An increasing body of evidence indicates that miRNAs are involved in the plant drought stress response
Environmental stressors due to climate change, especially drought stress, could make forests increasingly vulnerable to disease and die-offs
Only 234
Illumina sequencing of
According to a previous study on the relative soil moisture content (RSMC) of water-deficient soil
Additional file 1: S1
Summary of
Click here for file
The size distribution of all unique sRNAs is summarized in Figure
Figure 1
Sequence length distribution of
Sequence length distribution of
After genomic annotation of the
Additional file 2: S2
30 undetected miRNAs in this study.
Click here for file
Novel non-conserved miRNAs from
After identifying potential siRNAs and conserved miRNAs from the unique sRNA sequences, the remaining sRNA sequences were potential candidate miRNAs. For the identification of new miRNA, the primary criterion was a stable hairpin structure. After pooling the reads of the two libraries and analyzing the precursors of potential miRNAs using the MFOLD web server, we found 274 potential candidate miRNAs (Additional file
Additional file 3: S3
Potential miRNA candidates without miRNA*s found only in one library of
Click here for file
These RNA structures were predicted by MFOLD software and manually checked according to the criteria of Meyers
Figure 2
Predicted miRNA precursor' hairpin structures of new miRNA precursors
Predicted miRNA precursor' hairpin structures of new miRNA precursors. Precursor structures of 4 newly identified poplar miRNAs (miRn5, miRn11, miRn38, and miRn50) were predicted by MFOLD pipeline. The MEFs were list after the miRNAs name. The mature miRNA and miRNA star sequences are highlighted in red and blue, respectively.
Additional file 4: S4
The predicted hairpin structures of all the 65 new miRNAs precursors.
Click here for file
Differential expression of miRNAs in
To identify drought-responsive miRNAs from
Additional file 5: S5
MiRNAs in response to drought stress.
Click here for file
Figure 3
Differential expression analysis of conserved and novel drought-responsive miRNAs
Differential expression analysis of conserved and novel drought-responsive miRNAs. The changes in miRNAs for CL and DL are greater than 2-fold. For each miRNA, sequence reads were divided by the total sequence number then multiplied to 1,000,000 (reads per million). Differential expression of known and new miRNAs in response to drought stress by sequencing is shown in panel A. The positive and negative values mean miRNAs whose expression was stimulated and suppressed by drought stress, respectively. ** mean significant difference between control and drought stress at P ≤ 0.01. The relative expression level of miRNAs measured by RT-qPCR in response to drought stress is shown in panel B.
We further analyzed the expressions of the 65 new miRNAs under the two treatments. The drought-responsive miRNAs are listed in Figure
Target analysis of novel and conserved miRNAs by degradome sequencing
The previously known miRNA targets also identified in this study are available on the PopGenIE site (
Additional file 6: S6
Targets prediction of new
Click here for file
The verification of miRNA targets would provide further evidence for the existence of new non-conserved miRNAs. To identify the miRNA targets, two parallel analyses of RNA ends (PARE) libraries were constructed for the
Additional file 7: S7
Summary of
Click here for file
Plant miRNAs have a strong propensity for target genes with important functions
High-throughput sequencing of
In a comparison of six
Novel miRNAs
Compared to six previous studies of
Drought-responsive miRNAs in
Although miRNAs have been shown to play an important role in the drought stress response of
We further studied the target genes of these drought-responsive miRNAs by sequencing of the degradome library and comparing our work to previous studies
Ptc-miR473 was also upregulated in drought stress. It targets a member of a plant-specific GRAS transcription factor gene family
The number of downregulated miRNAs was larger than the number of upregulated miRNAs. The two downregulated miRNAs (miR160 and miR164) were both identified to be cold-responsive miRNAs in
Two downregulated miRNAs (Ptc-miR408 and Ptc-miR1444) have been reported to be Cu-responsive miRNAs in
Other downregulated miRNA is Ptc-miR394, whose predicted targets are annotated as dehydration-responsive protein (POPTR_0002s07760.1) and F-box proteins (POPTR_0001s13770.1 and POPTR_0003s16980.1), which were recently reported to be differentially regulated by stress conditions and to play significant roles in the abiotic stress-response pathway. In
From the analysis of predicted targets to downregulated Ptc-miRn6, a CCCH-type zinc finger protein and two trichome birefringence-like proteins (TBLPs) were functionally predicted. Although a cotton CCCH-type zinc finger protein has been identified to enhance abiotic stress tolerance in tobacco
Degradome analysis of non-drought-responsive miRNAs
The degradome sequencing results imply that the miRNAs with no detected targets may silence genes by repressing translation. However, we could not obtain information about translation repression by miRNA through degradome sequencing. Only 19 targets of new miRNAs were identified. The targets of these non-conserved miRNAs are difficult to detect, possibly because of low abundance or a spatial expression pattern. More studies are needed to shed light on the regulation network of these miRNAs in
In this study, sRNA libraries and degradome libraries of control and drought treatments were constructed with poplar leaves for high-throughput sequencing. Twelve miRNA members in 11 families were confirmed to be responsive to drought stress, and 65 novel miRNAs with star sequences of 59 families were identified. Through degradome sequencing, 53 and 19 genes were identified as cleavage targets of annotated miRNAs and new miRNAs, respectively. The functions of miRNA targets were analyzed and discussed. This study provides useful information for further analysis of plant miRNAs and drought stress tolerance, particularly in
Plant materials and total RNA extraction
Additional file 8: S9
Leaf water potential.
Click here for file
Additional file 9: S10
Leaf photosynthetic data.
Click here for file
High-throughput sequencing and bioinformatics analysis
Illumina sequencing on sRNAs (ranged from 18 nt to 30 nt) was conducted using an Illumina Genome Analyzer, following the Illumina protocol
Differentiatial expression analysis of miRNAs between the two treatments
The sequence reads of the two libraries were normalized to 1 million by the total number of sRNA reads in each sample. The calculation of the
The following
where N1 is the total number of reads in the sequencing library of the control, N2 is the total number of reads in the sequencing library of the drought treatment, x is the number of reads for an miRNA in the control library, and y is the number of reads for an miRNA in the drought treatment library.
All calculations were performed on a BGI Bio-Cloud Computing platform (
RT-qPCR of mature miRNAs
To validate the results of miRNAs from high-throughput sequencing, RT-qPCR was performed. The RNAs were extracted from leaves using the CTAB method
Additional file 10: S8
The primers designed for RT-qPCR.
Click here for file
Target prediction and confirmation by degradome sequencing
Degradome sequencing following the PARE protocol was used
miRNA: MicroRNAs; P. trichocarpa:
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
PS DL designed and conducted the experiments, PS DL ZZ analyzed the data, PS DL WY XX drafted the manuscript, WY XX supervised the project. All authors have read and approved the final version of this manuscript.
The authors would like to thank Feng Chen for providing convenience for use of BGI Bio-Cloud Computing platform. This research was supported by the Hi-Tech Research and Development Program of China (2013AA102701), the National Natural Science Foundation of China (31070597, 31270656), the Ministry of Science and Technology of China (2009CB119101), and the Scientific Research and Graduate Training Joint Programs from BMEC (Stress Resistance Mechanism of Poplar).
Cleavage of scarecrow-like mRNA targets directed by a class of
Identification of virus-encoded microRNAs
MicroRNAs: genomics, biogenesis, mechanism, and function
Essential role of MYB transcription factor: PvPHR1 and microRNA: PvmiR399 in phosphorus-deficiency signalling in common bean roots
MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for
Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes
Deep Annotation of
Genome-wide profiling of novel and conserved
Microarray-based analysis of stress-regulated microRNAs in
Genome-wide identification and analysis of drought-responsive microRNAs in
Salt and drought stresses induce the aberrant expression of microRNA genes in tobacco
Genome-wide characterization of new and drought stress responsive microRNAs in
Expression profiles of precursor and mature microRNAs under dehydration and high salinity shock in
Identification of drought-responsive microRNAs in
Genome-wide identification and expression analysis of heat-responsive and novel microRNAs in
Genome-wide identification of cold-responsive and new microRNAs in
Impact of drought on productivity and water use efficiency in 29 genotypes of
Identification of novel and conserved
A global overview of drought and heat-induced tree mortality reveals emerging climate change risks for forests
Genome-wide responses to drought in forest trees
The genome of black cottonwood,
Stress-responsive microRNAs in
Genome-wide profiling of
miRBase: integrating microRNA annotation and deep-sequencing data
Plant responses to water stress
Novel and mechanical stress-responsive microRNAs in
Conservation and divergence of microRNAs in
A species of small antisense RNA in posttranscriptional gene silencing in plants
Criteria for annotation of plant microRNAs
Sorting of small RNAs into
Origin, biogenesis, and activity of plant microRNAs
Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences
MicroRNA-directed phasing during trans-acting siRNA biogenesis in plants
Specific effects of microRNAs on the plant transcriptome
Construction of Parallel Analysis of RNA Ends (PARE) libraries for the study of cleaved miRNA targets and the RNA degradome
Endogenous siRNA and miRNA targets identified by sequencing of the
ABA induction of miR159 controls transcript levels of two MYB factors during
MicroRNA Expression Analysis in the Cellulosic Biofuel Crop Switchgrass (
Overexpression of gma-MIR394a confers tolerance to drought in transgenic
Regulation of barley miRNAs upon dehydration stress correlated with target gene expression
microRNAs associated with drought response in the bioenergy crop sugarcane (
High throughput sequencing reveals novel and abiotic stress-regulated microRNAs in the inflorescences of rice
miR398 and miR408 are up-regulated in response to water deficit in
Modulation of gene expressions and enzyme activities of methionine sulfoxide reductases by cold, ABA or high salt treatments in
CaMsrB2, Pepper Methionine Sulfoxide Reductase B2, Is a Novel Defense Regulator against Oxidative Stress and Pathogen Attack
OsMSRA4.1 and OsMSRB1.1, two rice plastidial methionine sulfoxide reductases, are involved in abiotic stress responses
Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in
The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in
The VEP1 gene (At4g24220) encodes a short-chain dehydrogenase/reductase with 3-OXO-Delta(4,5)-steroid 5 beta-reductase activity in
Involvement of the VEP1 gene in vascular strand development in
Specific enrichment of miRNAs in
Control of root cap formation by microRNA-targeted auxin response factors in
Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages
Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in
Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions
Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice
Conservation and diversity of microRNA-associated copper-regulatory networks in
Spatiotemporal analysis of copper homeostasis in
GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5
TRICHOME BIREFRINGENCE and its homolog AT5G01360 encode plant-specific DUF231 proteins required for cellulose biosynthesis in
Induction of glandular and non-glandular trichomes by damage in leaves of
Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions
Triggering the formation of tasiRNAs in
Effects of silicon on defense of wheat against oxidative stress under drought at different developmental stages
A red beet (
Perturbation of indole-3-butyric acid homeostasis by the UDP-Glucosyltransferase UGT74E2 modulates
Characterization and expression profile of two UDP-glucosyltransferases, UGT85K4 and UGT85K5, catalyzing the last step in cyanogenic glucoside biosynthesis in cassava
A simple and efficient method for isolating RNA from pine trees
A large genome center's improvements to the Illumina sequencing system
SOAP2: an improved ultrafast tool for short read alignment
Rfam: Wikipedia, clans and the "decimal" release
miRBase: tools for microRNA genomics
Mfold web server for nucleic acid folding and hybridization prediction
The significance of digital gene expression profiles
POWER_SAGE: comparing statistical tests for SAGE experiments
Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method
MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5 ' region
Principles of MicroRNA-target recognition
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs
CleaveLand: a pipeline for using degradome data to find cleaved small RNA targets


text:
I don't own these characters. Oh, wait, I do own Dawn and Kendra, but everyone else belongs to Disney.
Epilogue: The law was on her side
The morning was calm, so the assault came without warning as far as Dawn Killigan was concerned. She had been in her kitchen, getting coffee when out of the blue Doctor Director and Shego joined her. Her eyes went wide as they pulled up chairs at her kitchen counter, flanking her.
"Where's the baby?" Shego asked, as if what was happening was utterly normal. She leaned an elbow on the counter and rested her head in her palm, looking almost curious to go along with her question.
"How did you two get in?" Dawn demanded to know, an edge of panic in her voice as she looked back and forth between the uninvited women. She had an excellent security system, so she wanted to know how they possibly breached it.
Shego rolled her eyes to the question. Obviously, Dawn had not completely done her homework on who she was messing with. Big mistake, both intruders thought.
"That's not the issue at hand. The issue at hand is that you thought you could waltz around ruining Kim's good name and then leave like nothing was going to happen," Doctor Director commented in a business-like tone, which sort of hid her anger. The frown on her face did imply her current emotional state, though. And there was the glare that was coming from her onyx eye, even though she was not aware of it.
"I haven't committed a crime," Dawn pointed out, calming down to make sure she was not on the defensive. She was sitting straight up, like there was a rod in place of her spine, so she was still giving off quite the nervous air.
"And, what's your point?" Shego asked curiously, an eyebrow high on her brow. So, she did not think she committed a crime? Who said she did? Certainly not Shego; since if it was a crime was neither here nor there as far as Shego was concerned.
"You don't have any authority over me. I simply made a mistake—" Dawn started, obviously having her excuse all mapped out. Unfortunately for her, her guests were not interested in hearing it.
"You're damn right you made a mistake. You made a mistake the moment you targeted my Kimmie for this bullshit plan you had, dragging her name through the mud and all of this other crap. Now, you knew for a fact you hadn't slept with her, so you knew that baby wasn't hers," Shego stated soundly.
"Therefore you willingly and maliciously lied about Kim," Doctor Director chimed in, sounding just as serious as her momentary partner.
"Well, as you Americans say, so sue me," Dawn remarked, smirking as her air of confidence and arrogance began to return to her. A haughty, amused look invaded her sky-colored eyes.
"The thought had crossed my mind. I could use trust fund money as napkins at the dinner table," Shego retorted thoughtfully. It seemed like it would be quite fun to take Dawn to court and strip her of every dime that she had due to all of the emotional suffering she had caused Kim and all of the lying she did, but that idea was shut down by her lover when she started pitching it.
"But, making you poor would hurt your daughter and Kim was utterly against that," Doctor Director explained in a tone that showed she really did not care. In fact, she was mentally damning Kim's kind heart because she believed that Dawn desired to be crushed for such a horrible stunt that could have cost Kim so dearly.
Dawn's eyes went wide for a moment, letting the duo know that they struck a cord within her. She frowned just from the mention of her daughter and glared at the both of them as if she was trying to will their deaths right then and there. They better not try anything to separate her from Kendra, she thought.
"So, we'll settle this out of court," Shego said, smirking demonically.
"Way out of court," Doctor Director concurred, an expression quite similar to the former thief.
"What? You're going to bully me?" Dawn inquired. She scoffed and rolled her eyes.
"No, we're going to get you to go on international television and admit why you did all of this. Admit that Kim is not the horrible person you made her out to be, and that you were trying to hurt her," Doctor Director replied in a dead serious tone.
"Or else what?" the redheaded Scot demanded to know.
"Or little baby Kendra goes bye-bye," Shego commented, having the nerve to wave at the end of that statement.
Dawn snarled and turned to grab Shego. The former villainess caught Dawn's hand and pressed on her wrist. Shego locked eyes with Dawn, each showing their most serious expression. For the first time since they met, Dawn blinked.
Doctor Director started talking while Shego continued to glare at the Scottish woman. "You caused a lot of trouble, and for you to think that you could just walk away after the test results come in with nothing happening to you is a dream that you're being rudely awakened from," Doctor Director said.
"And you think this will solve the problem?" Dawn asked bitterly. She knew that she unleashed a dragon that could not be put back in the box completely. Kim was forever tarnished and that was good enough for her. Her father was still avenged because no one would look at Kim quite the same way thanks to her.
"It'll make things better than they are now. And don't think that's the end of things," Shego replied strongly, still gripping Dawn's wrist. She put a bit of pressure on the part, causing the redhead to hiss in pain.
"Wha-what else?" Dawn asked while trying to hide her discomfort. She was failing miserably as her mouth was bent up into a grimace, her eyes were slightly wide from pain, and her brow creased from tension. She was also twisted to the side, trying to alleviate the pain shooting out of her wrist.
"Doesn't matter at the moment. Right now, get ready for you press conference," Doctor Director commanded.
"What? Right now?" Dawn asked in shock through gritted teeth.
"We'd like to get to that as soon as possible. Well that or get to putting the ball in motion for you to lose Kendra. Whichever one you want us to do first," the leader of GJ commented calmly.
Dawn scowled, her anger overriding the pain in her wrist. "Fine," she hissed.
"We'll discuss other punishments later. But, just be aware that I'm watching you now and I might only have one eye, but I see everything. If you so much as jaywalk anywhere in the world, I'll be on your case and you'll be that much closer to not having a daughter," Doctor Director promised.
Dawn did not respond. They had her. She refused to even tempt fate when it came to Kendra now. Vanessa made her see that her daughter was the most precious thing that she had and she wanted Kendra to think the world of her when the baby grew up; just like she thought the world of her father. She did not want to disappoint Kendra in any way, shape, or form because she knew how horrible that feeling was for a child; she imagined it was just as bad for a parent if the parent was aware of the disappointment.
So, they had her. She did whatever they wanted. They knew that she would.
Shego wore a small smile on her face as she moved around the kitchen of the apartment; she was practically gliding through the room. The smile grew as she heard the front door open, so she knew that Kim had come home. Moments later, arms wrapping around her middle confirmed her thoughts.
"Had a good day at work?" Shego asked curiously.
"Not really. We didn't get a new case, so it was mostly paperwork and getting my team to stop having a water fight in the building," Kim explained, cuddling into her lover's shoulder.
"Your team is full of little kids. How do you get them under control long enough to finish a case?" Shego remarked. Sometimes, she thought it was like her girlfriend ran a kindergarten, complete with naptime for her team.
"Promises of ice cream at the end of the day," Kim joked.
Shego chuckled a bit and turned around to hug her girlfriend properly. They shared a touching, sweet kiss and then broke apart as Kim went to go take a shower. They then had a quiet dinner. After that, they cuddled up on the couch. Shego turned on the television, her favorite news channel was on.
"Oh, come on!" Kim groaned when she saw who was on television. "What the hell is she going on about now?" The "she" that she was referring to was Dawn Killigan.
"Let's see what she's got to say," Shego commented, hiding a smirk that was trying to fight its way onto her face.
"Do we have to?" Kim groaned.
"I doubt she's making any more horrible claims about you. After all, we already had the DNA tests and everything," the pale woman pointed out.
The younger woman did not argue and decided to just tune Dawn out while Shego watched. Shego could not keep back the smirk as Dawn started talking. The Scottish woman told the world who her father was and his imprisonment started her hatred of Kim Possible. She spoke about how she took a long time, thinking of getting revenge against the hero. These words caught Kim's attention and she turned to listen to the rest of the show.
Dawn went on, explaining how she lied about sleeping with Kim. She did state that she knew for a fact that Kim was not the one that got her pregnant. And when it was all said and done and she was asked why she was coming clean now, she explained that she felt guilty.
"You know, while all of this was going on, Kim spent time with my daughter and she was so good with Kendra. After a while, I felt guilty about ruining not only her name, but her life with this farce. She's far too good a person for me to just leave things with most of the world thinking that she's done these things when she hasn't done any of them," Dawn responded to the question.
"Guilt? She expects us to believe that she actually feels guilt?" Kim shouted incredulously. She would be surprised if Dawn felt anything at all!
"Kimmie, Kimmie, Kimmie," Shego said while pulling the redhead closer to her. She rested her cheek on top of Kim's head.
Kim tried to turn to look at her lover curiously, but gave up when she realized that Shego's face was in her hair. "What?"
"What does it matter why she confessed? The fact of the matter is she did confess and now everyone knows that she was just playing you. That's what counts," Shego pointed out.
Kim conceded that point. "I guess you're right."
"I'm always right. Haven't you noticed?" Shego remarked with a smile.
The redhead scoffed. "I've noticed some flaws every now and then."
"Well, we can't all be little Miss Perfect. But, we can put this behind us now," Shego pointed out.
"Thankfully," Kim sighed, her shoulders flopping into relaxation.
The younger woman put her hands around Shego and brought up a leg to wrap around Shego as well. The pale woman put a hand on Kim's thigh while the other used the remote to turn off the television.
"You know what would really ruin this moment right now," Kim commented.
"Your parents calling again asking about grandchildren?" Shego guessed.
"Yeah," Kim concurred.
"You said they were going to let that go. It's been two weeks. When are they going to start letting it go?"
"I thought they would. I'm starting to think they're serious. So, I started thinking, maybe we should start thinking about it."
"Kim Possible, are you trying to say that you want to have my baby?" Shego asked in a teasing tone, even though on the inside she was flattered. It amazed her that Kim would trust her enough to think about having a baby with her after she walked out on Kim at her most trying moment.
"Well, if that's how it happens. Who's to say you wouldn't be having my baby?" Kim countered with a smile.
"And ruin my figure? Fat chance," Shego quipped, laughing a little as she spoke.
"Well, I can't chance missing work. My team wouldn't even bother coming in if I show any sign of changing," Kim argued with a smile.
"Well, then I guess we just won't be having kids."
"Well, then I guess we won't," Kim agreed and then the phone rang. They looked at each other, exchanging knowing and horrified glances. "So, yeah, you tell my parents that."
"Like hell, they're you're parents!"
"We have got to work this out," Kim said. They had exchanged one pest in Dawn for two in her parents. But, at least her parents were not out to ruin her whole life. They were looking to change it, though. They really wanted grandchildren apparently.
The end.

subset: commoncrawl


text:
By Jack Carey, USA TODAY
As losses become more plentiful than they used to be, opponents start winning a few more recruiting battles and ticket sales aren't as brisk as they once were, college football coaches can find themselves fending off talk of hot seats and employment prospects.
There never seems to be an offseason for speculation on which coaches will survive or who will be moving on.
And in a world where fans want success and want it yesterday, patience is in short supply. After only a month of the season, Internet fan forums and talk radio shows are already overflowing.
Phillip Fulmer is the subject at Tennessee, where the Volunteers are 1-3. Greg Robinson, 8-32 in his fourth year at Syracuse, is as good as gone, if many fans are to be believed.
Virginia's Al Groh is a target after a 1-3 start that includes lopsided losses to Connecticut and Duke. Washington's Tyrone Willingham is a loss away from the Huskies' first 0-5 start in almost four decades. Clemson was picked to win the Atlantic Coast Conference, but coach Tommy Bowden's club is a disappointing 3-2, including 1-1 in the league.
In the midst of it all, coaches have to keep themselves and their players concentrating on upcoming opponents.
"I'm not real aware of the reports on my job status, maybe the kids are more in tune," says Iowa coach Kirk Ferentz, 15-15 since 2006 after going 38-12 the previous four years. "We're just trying to get better and win some football games here."
American Football Coaches Association executive director Grant Teaff calls all the griping "the dark side of college football."
"Our game has never been more popular nor more viewed, but the stakes are extremely high. The higher the salaries, the shorter the patience. It goes with the territory. You would hope that could be avoided, but we just see so much pressure now."
Contributing: Andy Gardiner
Guidelines: You share in the USA TODAY community, so please keep your comments smart and civil. Don't attack other readers personally, and keep your language decent. Use the "Report Abuse" button to make a difference. Read more.


text:
What is JotForm?
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Translation of Already built formAsked by Javidosmanli on March 13, 2015 at 12:56 PM
To whom it may concern,
I have created form in english, and I would like to translate it to german. I have my own translations.
How can I do it?
or I have to create new form in german again.
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thank you
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text:
Shipping Instructions for Returning Instruments
Use our On-Line Service Request Form to get an RMA number and let us know what instrument(s) you are sending. Include a copy of the form with the instrument when you ship it, and include the RMA number on the outside shipping label.
Please read instructions on:
- Shipment methods (below)
- Instrument crates for shipments to the European Union or China (below)
- Shipping Batteries — Lithium or Nickel-Metal Hydride (NiMH)
- Preparing SBE 25 or 9plus Temperature / Conductivity Sensors for Calibration (removing TC Duct)
Shipment Methods
1. DOMESTIC SHIPMENTS (USA) - SHIP PREPAID (via UPS, FedEx, DHL, etc.) directly to:
Address (directions):
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Telephone: (425) 643-9866
Fax: (425) 643-9954
International Shipment Option A — Ship via PREPAID AIRFREIGHT to SEA-TAC INTERNATIONAL AIRPORT (IATA CODE "SEA"):
- Address (directions):
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Telephone: +1 (425) 643-9866
Fax: +1 (425) 643-9954
E-mail: firstname.lastname@example.com
- NOTIFY: MTI WORLDWIDE LOGISTICS for Customs Clearance
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- Telephone: (+1) 206-431-4366
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E-mail: firstname.lastname@example.com
- Please e-mail the flight details and airway bill number to email@example.com and MTI Worldwide (email@example.com) when your shipment is en-route to Sea-Bird. Include your RMA number in the e-mail.
International Shipment Option B — Ship via EXPRESS COURIER directly to Sea-Bird Scientific
Address (directions):
13431 NE 20th Street
Bellevue, Washington 98005, USA
Telephone: +1 (425) 643-9866
Fax: +1 (425) 643-9954
If you choose Option B, we recommend shipping via UPS, FedEx, or DHL. Their service is door-to-door, including customs clearance. It is not necessary to notify our customs agent, MTI Worldwide, if you ship using a courier service.
Please e-mail the airway bill / tracking number to email@example.com when your shipment is en-route to Sea-Bird. Include your RMA number in the e-mail.
Include a Commercial Invoice showing the description of the instruments, and Value for Customs Purposes Only. Include the following statement:
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Failure to include this statement in your invoice will result in U.S. Customs assessing duties on the shipment, which we will in turn pass on to the customer/shipper.
Instrument Crates
Some years ago, emergency measures for coniferous (soft wood) Non-Manufactured Wood Packing Materials (NMWP), also referred to as Solid Wood Packing Materials (SWPM), went into effect in China and the European Union (EU). These measures, applicable to all wood crates, pallets, skids, drums, cases, etc., were designed to prevent the introduction of the pinewood nematode, a microscopic eelworm that has caused extensive mortality in pines in Japan and China. These measures applied to all coniferous woods in shipments originating from the United States, Canada, China, and Japan.
In response to those regulations, Sea-Bird substituted mahogany (a tropical hardwood that was exempt from the regulations), or Manufactured Wood Product such as plywood (also exempt from the regulations), for the fir previously used for the edges of our crates.
In 2002, the Food and Agriculture Organization of the United Nations published "International Standards for Phytosanitary Measures, Publication #15, Guidelines for Regulating Wood Packaging Material in International Trade" (ISPM 15). ISPM 15 regulations require that any wood packaging material be heat treated to the approved standard. Check your local laws for implementation of these regulations.
The American Lumber Standards Committee (ALSC) developed a U.S. standard that meets or exceeds the requirements of ISPM 15 (https://www.ippc.int/publications/regulation-wood-packaging-material-international-trade-0). Packaging that meets the requirements of ALSC is marked with the ALSC stamp on a minimum of two opposing faces of the completed packaging product.
Starting in 2004, all packaging leaving Sea-Bird for international shipments of new products met the new standards. Packaging is either:
- marked with the approved certification stamp, or
- built entirely of Manufactured Wood Products (such as plywood), and is therefore exempt from the treatment and marking requirements
By early 2007, Sea-Bird used up inventories of existing non-compliant boxes, and switched to using approved packaging for all shipments (including in the U.S.) using wood boxes.
Beginning in 2004, the United States started enforcing ISPM 15. If a customer tries to send in equipment for servicing in a non-approved wood box, it may not be allowed into the U.S. If it does reach Sea-Bird, we will return the instrument in a new crate that meets the requirements of ISPM 15. We will charge $50 to $150 for the replacement crate, based on the crate type. These prices are valid only for crate replacement required in conjunction with return of a customer's instrument after servicing, and only when the instrument was shipped in a crate originally supplied by Sea-Bird.
Additional Information
- Warranty Policy
- Preparing SBE 25 or 9plus Temperature/Conductivity Sensors for Calibration (removing TC Duct) — Read before packing instrument for shipment to Sea-Bird.
- FAQs — answers to commonly asked questions about servicing Sea-Bird instruments
- Sending Data to Sea-Bird — procedure for uploading files to a secure ftp site, for review by Sea-Bird.


text:
The Game's "Sunday Service" series has provided nothing but hits, and last night he kept the good times flowing with a new star-studded track entitled, "Rollin." The track features Kanye West on the sample, along with verses from Southern soldiers Trae Tha Truth, Z-Ro, Paul Wall, and Slim Thug. You can stream and download the track after the jump.
We only have one more Sunday until Jesus Piece hits stores, and I think it will be hard for Game to top "Rollin" in that time. Do you agree? Comment below and let us know!
James Shotwell
James Shotwell is the founder of Under The Gun Review. He loves writing about music and movies almost as much as he loves his two fat cats. He's also the co-founder of Antique Records and the Marketing Coordinator for Haulix. You should probably follow him on Twitter.
Latest posts by James Shotwell (see all)
- 2015's Most Underrated Films - December 29, 2015
- Passing The Torch: The End Of An Era For UTG - December 1, 2015
- UTG PREMIERE: Before The Streetlights – "Private Browser" - November 26, 2015


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"Darragh Gammell" <dbgsyste...@gmail.com>
|Re: $m->session
|Wed, 12 Dec 2007 00:57:13 GMT
>From the mason devel Manual we see: $m, the Mason request object, provides an analogous API for Mason. Almost all Mason features not activated by syntactic tags are accessed via $m methods. But looking under "HTML::Mason::Request - Mason Request Class" there is no reference to $m->session. This is where I'm getting confused. Is it possible that whomever wrote this website created their own Object called $m->session and thats why I cant find it anywhere? My Apologies for the silly questions, trying to get to grips with new technologies with no developers present. Regards Darragh On Dec 12, 2007 11:48 AM, Aaron Dalton <firstname.lastname@example.com> wrote: > $m->session is a Mason construct. > > -- > Aaron Dalton > http://perlkonig.com > >
|View raw message


text:
The whole idea of the Tampa Bay Buccaneers trading down has always been silly. They are in excellent position to finally draft a potential franchise quarterback and they would pass that up? Not a chance.
SI's own Peter King wrote an excellent article on Monday in regards to the Bucs' potential draft selection of Florida State quarterback Jameis Winston. In it he quoted a few things from his conversation with general manager Jason Licht. But on ESPN Radio Tuesday, King revealed something that he did not include in his column.
"The one thing that I really didn't write Monday in my column was that -- we talked about what if they got some kind of great offer for [the No. 1 pick] and [Jason Licht] said they are leaning very, very strongly one way," King told ESPN's Colin Cowherd. "He didn't say which way, but it's clear [based on their conversation] that they are leaning toward, no matter what kind of offer they get, they are taking one of those guys (Winston or Oregon's Marcus Mariota)."
So there you have it. Licht, through King, has single-handedly shot down any high hopes of trading down and stocking up picks. That debate should end today. Many have been in agreement as well that there was no chance of the organization trading that No. 1 selection.
For some time now, the consensus has been that either Mariota or Winston will be selected first overall. The Bucs would be wise to stay put and it appears that will undoubtedly be the case now.
Follow Bucs Blitz on twitter: @TBBucsOnScout


text:
Company Overview Aftersales Solutions Partner
- Gervais Sutton
- 2 years ago
- Views:
1 Company Overview Aftersales Solutions Partner Jerry Schmidt Vice President, Global Business Development Q2-2015
2 I: I: Who is is APS? II: History III: HP, Vertical Markets, B2B, OEMs IV: Core Competency V: Why APS? VI: APS Proposition Maximize Client Value Backup
3 I: Who is APS? APS is your E2E Aftersales service partner specializing in Global Asset Recovery of Consumer Electronics and Enterprise computers. APS excels in today s cutting edge technology of wireless solutions involving mobile devices & smartpads. APS manages in/outbound shipments and warehouse inventory. Triage, upgrades & repairs, and kitting & packaging is customized to your requirements. APS utilizes Lean Six Sigma methodology to cut waste with efficient processes. Our Engineers provide value-added services such as Root Cause Analysis and 8D Reporting. APS recaptures the value of your returns that maximizes profit!
4 II: History - Growth 1998 R.E.L. Tech establishes Houston operations 2000 Sam Direct Group, PLC purchases operations 2000 BSI provides ISO 9001:2000 certification 2003 APS established with MBO 2003 HP Remanufacturing Services Quality Supplier of the Year Award 2004 HP PSG Remanufacturing awards PC RMA process 2004 HP PSG Commercial Remanufacturing Quality Supplier of the Year Award 2006 HP Handheld GBU establishes AT&T carrier engagement model 2007 APS receives HP Handheld GBU NPI agreement involving Inventec 2008 APS receives AES (Automated Export System) Compliance Certification 2008 APS hit by Hurricane Ike, but continues to provide 24/7 support 2009 APS and HP enters in a Service Business Agreement for Handhelds support HP awards Handhelds Logistics support from HTC, LGE, Foxconn & Pegatron 2014 HP awards Handhelds Call Center & Empty Box support from Next & Zoyto
5 II: History - Expertise Production Staff a. Green Belt Trained b. BSI Auditor Trained c. Microsoft Certified Technology Specialist d. Average of years experience Management Team a. Finance - MBA b. IT MSIT c. Business Development PMP, Master s Certification in Project Management d. Average of years experience
6 III: HP Product Lines of Business HP Remarketing Americas PSG/PPS a. PCs - Desktops, Thin Clients b. Mobile - Laptops, Handhelds c. Displays d. Flat Panel TVs HP Handheld Global Business Unit (GBU) a. Smartphones b. Tablets/PDAs c. Navigation Devices
7 III: HP Value Added Services HP Remarketing Americas PSG/PPS a. CI Direct and Indirect b. Triage/Repair/Refurbish c. Reporting d. Pick/Pack/Ship Fulfillment HP Handheld Global Business Unit (GBU) a. E2E Support NPI through EoSL ODM relationships Material Procurement b. Warranty c. Extended Service Contracts
8 III: HP Vertical Markets CAN USA Rogers Telecom ATT, St. Jude, Winder Farms LAR Movistar, DHL, Pfizer EMEA AP/J EDS, Vodafone, Mercedes-Benz Honda, Taiko-Seiko
9 III: Vertical Markets Case Studies American Greetings Customized Handheld SKU bundle made ready for resale St. Jude Medical US FDA certification, global Handheld Re-SKU, extended battery solution EDS Smartphone worldwide deployment Honda Japan Handheld lifecycle project
10 III: B2B Associations Flextronics Supplier to EMEA fulfillment hub HP Services Virtual UPS fulfillment hub Inventec Warehousing & on-demand kit creation Dell Hub inventory; Optical Drive upgrade Lite-On Rack power supply testing
11 III: B2B Peer Relationships Avnet IT operations Ryder Shop Floor Data Management Kewill SLS E2E service fulfillment tool HP BPIA partner agreement Digital Badge DecisionOne Motherboard Testing & Repair
12 III: OEM Relationships HTC LGE Inventec / IAC Compal / CCI Foxconn Pegatron
13 IV: Core Competency E2E Solutions
14 IV: Core Competency - Triage, Test, Repair Receipt of goods / De-trash Screen (Sort & Triage) Test/Report Repair/Refurbish Remarket Kitting Packaging On-Demand Printing Fulfillment & Logistics Pick/Pack/Ship Hubbing Transportation Import/Export
15 IV: Core Competency E2E IT Solutions Real time Inventory available via Web. Products uniquely tracked in the APS shop floor system from receiving to ship. EDI and AS2 integration capability with other external partner systems. Unbiased data reporting and performance matrix. In-house IT team incorporates Client s requirements into new business processes flow.
16 V: Why APS? Ready-To-Go Services Recall/Recovery/Rework Programs Local customer support & repair Ad-hoc / hot shot Projects Long-Term Support NPI Launch Programs Sustaining Lifecycle Programs Cost effective logistics bulk RMA shipments vs. end-user transactional End of Service Life disposition Outstanding Quality
17 V: Why APS? (Quality & Reporting) Engineering Quality and Reporting Case Studies: Issue #1: Device will not charge Root Cause: USB connector shearing off motherboard during normal use Issue #2: no audible notification/alarm Root Cause: Audio jack stuck in open position when earphones removed APS Engineers: Performed root cause analysis Performed 8D Reported findings with Client
18 VI: APS Proposition Maximize Client Value Low Financial Risk Flexible Operations Quality TCE
19 VI: APS Proposition Maximize Client Value Low Financial Risk APS owns the support Inventory APS bills the client per transaction APS experience maximizes the client s recovery value Flexible Operations Full E2E Aftersales support Flexible RMA flows Entire product life-cycle support Quality Fix it Right the First Time! Engineering Analysis Eliminate Repeat Returns Total Customer Experience Provide Effective Technical Support Delight the Customer Improve Client s Brand Loyalty
20 Backup VI: APS Proposition Maximize Client Value
21 APS VI: APS Diversification Proposition Maximize Client Value Warehousing Distributor Automotive Oil and Gas Healthcare Retail Q/C Inspection Reporting RL Call Center Repair Sustainability Fulfillment Pick/Pack/Ship Specialty & Niche Services IT
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text: CCW 2014: Motorola Solutions looks to mission critical integrated solutions 29 Jul 2014 12:27 PM Motorola Solutions looks to mission critical integrated solutionsMotorola Solutions showcased how the mass of voice, data and video information can be integrated into a single unified stream for real-time use by business and mission critical end users at CCW.Motorola Solutions' aim is to enable communications centres to better anticipate, forecast and predict, and for actions in the field to become more informed and collaborative. Motorola themed its demonstrations around three key areas: public safety, transportation and the minerals and energy sector.The demonstrations sought to show how public safety critical responders can collaborate securely and in real-time using mission-critical intelligence to shift from reacting to what has just happened to being ready for what's next.The movement of people and cargo is a dynamic, real-time environment demanding quick decision-making from transportation teams in rail operations, airports and sea ports. Motorola's solution ensures the right person or package arrives at the right location at the right time.In the minerals and energy showcase, Motorola showed how organisations can protect their assets and reach team members at all times, become better equipped to reach production quotas and maximise extractions, and maintain worker safety.Paul Steinberg, senior vice president and CTO of Motorola Solutions, outlined some of the thinking behind the integrated solutions on display. He noted that what becomes important is the ability to secure a single operational view, which can then be successfully communicated in real time for a unified response.He said that Motorola is working with Ericsson to take the most useful features from both TETRA and LTE applications in order to generate the next advances in public safety.Future mission critical networksSteinberg said future public safety networks need to be designed for worst case scenarios, the need to provide 100% geographic coverage, and they need a heavy uplink traffic model to enable data and video to be sent to command centres from the field.He added that mission critical networks need dynamic role/incident prioritisation to enable vital voice or data to be reprioritised to get that information through in real time. The question of subscriber data ownership also needs to be addressed, as the emergency services need to control the information.Other issues that have to be addressed include: group calling/one-to-many communications – the current LTE standard does have a way of doing this through eMBMS (enhanced multimedia broadcast multicast services); and network resiliency.Manufacturers looking to introduce mission critical LTE products are having to work ahead of the LTE standard, which does not include key mission critical features available in TETRA.Steinberg said that Motorola and Ericsson favour an over the top (OTT) approach to enabling mission critical functions on LTE, as opposed to Huawei's eLTE approach, which 'embeds' solutions deeper in the network. Steinberg believes the OTT approach keeps things open and more flexible.Ericsson is looking after three layers: the LTE RAN (eNodeB base stations etc); the lower LTE core (MME – mobility management entity; and serving gateway); and the upper LTE core (HSS – home subscriber server, PGW – packet data gateway, PS PCRF/QoS broker – public safety specialised policy rules function/quality of service). All of this needs to be able to communicate with the range of mobile devices employed by end users.Motorola is concentrating its expertise on the two layers above: the first covers interoperability services, including mobile VPN, priority manager/unified services, land mobile radio interworking and potentially IMS (IP multimedia services).The second comprises the application layer covering regional and national public safety apps including legacy apps, broadband apps, NextGen 911/999 calling, multimedia computer aided dispatch, messaging, video, maps/LBS, RMS and PSTN.Both Ericsson and Motorola have said that most of the key issues such as push-to-talk over LTE, group calling/one-to-many broadcasting and direct mode have been solved. It remains to be seen how close their solutions will match the 4G standard as it evolves over the next few years.LEX 755Steinberg also introduced Motorola's latest 4G ruggedised handheld device – the LEX 755. The touchscreen device features Motorola workflow applications on top of the main Android operating system.He described the LEX 755 as a step towards the real-time crime centre of the future where police officers wearing bio-harnesses with sensors, body-worn cameras and augmented reality head displays could be relaying a wide variety of real-time information back to command and control centres.James Atkinson EditorLinkedIn |Google + |Email Leave a CommentPlease write code to prove you're humanMore on... LTE GeneralMWC17: Zyxel to debut new LTE and Wi-Fi indoor gatewayHuawei, Vodafone and Qualcomm create LAA ready networkNokia demos live private LTE network over CBRS shared spectrumMore on... Critical CommunicationsFinland and Norway sign cross-border TETRA communication dealCC Europe 2017: Hytera showcasing new base station and handheld radioMore on... EricssonMWC17: Ericsson expands 5G access and transport portfolioOperators trial intercontinental 5G roaming using network slicing Public safetyTCCA pinpoints key focus areas for LTE-based critical communicationsMotorola launches all-in-one single site TETRA digital radio system Southern Tyrol TETRA network gets a workout at Biathlon World Cup Latest in LTEMWC17: Zyxel to debut new LTE and Wi-Fi indoor gatewayHuawei, Vodafone and Qualcomm create LAA ready networkNokia demos live private LTE network over CBRS shared spectrum


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8 Reasons You Ought To Be Making More Long-Form Informationposted on 16 Aug 2016 09:23 by futurebusiness directory Knowledge
Yet, new research indicates the exact reverse. The truth is, Google rewards brands that post complete, important, and in depth long-form content.
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Lately, Backlinko examined now one million Google search results for more information about the best Search Engine Optimization practices. To many marketers long form content ranks higher in Google's search results — !
This can be a far cry from the smaller balls of content we've been attempting to package with key words for optimization. Content that was more performed better every time. That is probably because of the fact it fosters your brand's relevancy and creates more societal shares than brief form content. Google ranks sites according to their degrees of ability; long form content helps build your sphere of influence.
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Contrary from what many content marketers consider, long form content is a strong tool earn ' esteem to consumers, brands can use to foster credibility, and get more from content advertising efforts. In a content-soaked on-line marketplace, site supervisors rack their brains for ways to be noticeable in the bunch. The greatest method to make an optimistic impact in your consumers would be to show that the brand is a trustworthy source of advice that is useful. Brands that are credible earn assurance is 'ed by consumers.
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He says that everyone prints posts within the 500-800 range, and posts that this platitude is avoided by standout. In depth feature articles create more traffic, more hits, and more social media shares than briefer posts — a recipe for creating the " " effect that is viral.
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Long-form content may feel like cash investment and a huge time, but the yields are worth it. Optimizing your inbound advertising efforts with long-form content conserves your brand cash in the long run. Long- form evergreen content divides the competition and your brand and creates company leads for an extended period of time — optimizing the prospective yields on the investment.
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WOOSTER -- Leaves will be picked up in the following areas (section number noted after the street) on the dates noted: Akron Road (state Route 585), 7, Nov. 14, Dec. 9; Highland Park to Old Airport Road, 7, Nov. 14, Dec. 9; Albern, 8, Nov. 17, Dec. 13; Allandale, 7, Nov. 14, Dec.
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text:
Your answer is one click away!
I am facing issue with the Code which is framed with Class.
Basically before It was simply a file with functions defined in it.
So When I was trying to execute the file using command python filename.py, It is working fine as needed.
Code sample is as follows:
# Getting the tenant list
# Fetch the creation_date of tenant if exists
def get_tenants():
# Fetch tenant list
tenants_list = keystone.tenants.list()
# Fetch tenant ID
for tenant in tenants_list:
tenant_id = tenant.id
So as shown in aboce code in the file I am trying to call get_tenants function, Also it is working fine as needed with no error.
Now I have Created the Class then moved all the functions in to the same.
Above function is Rewritten as follows now.
def get_tenants(self):
# Fetch tenant list
tenants_list = keystone.tenants.list()
# Fetch tenant ID
for tenant in tenants_list:
tenant_id = tenant.id
Then I have called the Function as follows:
billing = BillingEngine()
But, now I am getting the error as follows:
root@devstack:/opt/open-stack-tools/billing# python new_class.py
Traceback (most recent call last):
File "new_class.py", line 281, in <module>
File "new_class.py", line 75, in get_tenants
tenants_list = keystone.tenants.list()
NameError: global name 'keystone' is not defined
Note: Will provide the full file if needed.
May be you must define this?
class Example(object):
keystone = Keystone()
def get_tenants(self):


text:
A former Roman Catholic priest of the Camden Diocese has been arrested on charges he sexually abused a child.
James Hopkins has been charged with aggravated sexual assault and endangering the welfare of a child.
The alleged victim, Jonathan Norton, who is now 17-year-old, says he was abused by Hopkins between the ages of eight and 10.
Hopkins was banned from the ministry in 1995.
In 1999 Norton settled a civil suit against Hopkins and the Camden Diocese for $600,000.


text:
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Industry News
EquineLUX - An Innovative Approach To The Development Of The Contemporary English Saddle Pads
Contemporary standards of the Equestrian Sports place high expectations on the horse riding accessories. High-tech materials, developed for the use in different industries, finds new uses in the manufacturing of saddle pads.
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The majority of horse riding accessories are made in China in accordance with one simple principle 'the cheaper the better'. Despite the fact that functionality of low-priced saddle blankets is technologically limited, they still remain in demand. As a general rule, this kind of cheap horse equipment grabs the attention of the beginner riders, who strive to save some money.
Another widespread belief is that a saddle, if properly chosen, can always fit well. However, the horse's age, lifestyle and certain drawbacks of professional training, may result in losing or gaining fat or muscle mass, improving or degrading horse's body condition. This leads to obvious problems with the fit of the saddle, which can in its turn result in sores, swellings, hard spots, or blisters.
A true professional would have a tendency to take such factors as breathability, shock absorption, comfortable fit, and sweat-wicking feature into account when purchasing saddle pad and half-pad. When the saddle is properly fitted with a SHOCK-REDUCING™ Non-Slip Saddle Pad with SHIM POCKETS (this EquineLUX™ LUXURY Saddle Pad's model was released in December 2012), it will be interacting with a horse's back through a kind of body-shaped buffer. Equipped with set of open-cell High Resiliency open-cell foam shims, this innovative Saddle Pad allows for the overall weight to be redistributed over a larger area, thus it reduces impact on the pressure points of the horse's body. HR foam inserts allow for the overall weight to be redistributed over a larger area, thus reducing pressure and providing shock absorption effect. This effect can be prolonged by timely adjusting the positioning and thickness of the shims within and around of the problem areas on the horse's loins and withers.
The use of SHOCK-REDUCING™ Non-Slip Saddle Pad with SHIM POCKETS compensates for most of the common saddle fit problems. However, it must be done conscientiously, so that none of the shims interfere with saddle fit. This is especially important if a saddle tree is too narrow. Adding an excessive number of shims may result in incorrect balance and poor centering of the rider causing undue pressure in the front or the back of the saddle.
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For more information, please visit our Web site at http://www.EquineLux.com
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