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Doctor insights on:
Causes Of Pimple Like Rash On Arm Or Leg
I have a rash that start off as a small pimple and evolves to look like a burn mark. It's on my legs, waist, arms(severe) and neck. What can this be?
Exanthems (= rashes): such as yours are often impossible to diagnose with out the MD having the advantage of " laying eyes on it." Photos of good resolution can help but won't supplant good clinical evaluation. There are so many possibilities it wouldn't help anything to try to narrow down with such little information. On the other hand, a good dermatologist usually can nail it in a few minutes in person. ...Read more
Rash (Definition)
Most bumps and blotches on a newborn baby are harmless and clear up by themselves. By far the most common skin problem in infants is diaper rash. Diaper rash is an irritation of the skin caused by dampness, urine, or feces. Most babies who wear diapers will have some type of diaper rash. However, there are other skin disorders that can cause rashes. These are usually not serious unless ...Read more
I have a rash all over my legs and arms. It isn't raised or have any pimple like look to it. Went to a PA who couldn't diagnosis it. Should I be worried?
Can't advise : I'm sorry, but it's impossible to give advice about rashes without actually seeing the rash. ...Read moreSee 1 more doctor answer
In the last week I have been waking up in middle of the night itchy al over body, mostly stomach arms and legs, also hav a pimple like rash on wrist?
Scabies: Sounds like scabies. See a derm for office confirmation and a prescription. ...Read more
My 18 month old has a pimple like rash around her diaper line and on her arms and legs as well as blisters on the bottom of her feet?
Many possibilities : Have her doctor take a look, as this sounds like a pattern that can accompany a viral or perhaps a bacterial infection. It might need time, or a prescription antibiotic cream/ointment, something else, or nothing. Give the doctor a a call and see what he advises. Hope this is helpful. ...Read more
Son got his vaccines and has red pimples on his arms, chest, and back not on legs or stomach. Could it be heat rash from fever or vaccine related?
Dermatitis: Your son needs to be seen by PEds to make sure it is not vaccine reaction. ...Read more
I have red bumps/ pimple like on my arms, some on legs and feet. It itches. What's the cause of this?
Keratosis pilaris : rubra. Keratosis pilaris occurs when the human body produces excess amounts of the skin protein keratin, resulting in the formation of small, raised bumps in the skin often with surrounding redness. Harmless. See a dermatologist if concerned. ...Read more
What can cause pimples to pop up more frequently than normal on my husbands arms and legs? Could it be some kind of bug bite? They don't itch.
It could be: It could be it needs to be looked at when he has the pimples/rash. ...Read more
I have tiny pimple like bumps on my arm and legs. They don't itch or bother me. When i scratch them they turn white. What could they be?
Keratosis pilaris: These are more of a cosmetic issue than anything serious. Some people hate them and use a loofa to scrape them off of the backs of their arms. They happen more in kids and teens and less in adults. You might try some low-potency Hydrocortisone cream to see if that might smooth out these little bumps on the skin. ...Read more
My baby has red pimple like bumps on her arm and leg, they look almost like bug bites. They just appear. What could they be?
Newborn rash: If your baby is a newborn, then it's most likely the common newborn rash erythema toxicum. The rash should clear on its own. No treatment is needed. However, there are similar looking rashes that do need medical attention, so check with your baby's doctor. Here's a link for pic and info: www.Webmd.Com/skin-problems-and-treatments/picture-of-erythema-toxicum-neonatorum. ...Read more
I have pimple like dots on my arms and legs they have all of a sudden appeared over the last month what could this be and how do I treat it?
Folliculitis?: Each hair follicle is related to a small gland to lubricate it and prevent dryin out. Sometime these can become inflammed and cause small red bumps. Get it checked out to be sure it isn't an allergic reaction or viral infection. ...Read more
Skin Rash (Definition)
A skin rash is a symptom in which a person has changes on part or all of his skin, such as color changes, bumps, blisters, oozing, peeling, ...Read more
Pimples (Definition)
Pimples are a kind of acne, and are a condition where oil gets trapped in a pore of the skin resulting in small area of ...Read more
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28 Feb Don't Miss Out on Pancake Day – Try Our Protein Pancake Recipes Instead!
Protein Pancake Recipes
Looking for protein pancake recipes? Wondering what protein pancakes are?! Well, you've come to the right place!
I absolutely LOVE protein pancakes for numerous reasons:
- Taste fantastic
- Great way to hit your macro's for the day
- Perfect post workout snack
- Low in calories and fats.
- Best guilt free treat to have any time of the day!
Protein pancakes are NOT carb ridden like their predecessors. They are made predominately with protein powder. I use Protein Princesses Vanilla flavoured Whey 90. Some of the best protein pancake recipes can be anything from 8g upto 38g of protein per serving!!….HINT Cinnamon Roll Pancakes 🙂
Here are some of my favs
- Body Coach Recipe Protein Pancakes with Greek Yoghurt, Berries and Honey – 8g Protein
Serves 1
1 banana, roughly chopped
1 scoop Protein Princess vanilla protein powder
1 egg
25g rolled oats
1 tbsp coconut oil
Greek yoghurt, blueberries and raspberries, to serve
Whizz up the banana, protein powder, egg and oats in a blender to make your batter.
Heat up half the coconut oil in a non-stick frying pan over a medium heat. Pour little puddles of batter into the pan – I usually get 3 pancakes, with about half the batter in the pan at once.
Cook for about 1 minute on each side. Remove and repeat the process with the rest of the batter.
Serve with a dollop of yoghurt and a few fresh berries.
2. Vanilla-Spiked Protein Pancakes (9 g protein)
The protein in these bad girl pancakes comes from three eggs and a half-cup of cottage cheese. A quarter-measure of flour holds it all together, and vanilla concentrate and coconut oil give the cakes an interesting — and long for commendable — flavor. Top with new natural product, your most loved nut margarine or Greek yogurt for a balanced feast. Photo and Recipe: Emma / Better with Cake
3. Cinnamon Blast Protein Pancake (38g Protein)
Instant oats, vanilla protein powder, egg whites and Greek yoghurt make up the protein-pressed base of these pancakes, while banana and cream cheese make a wanton flavour and texture. Store in the freezer for breakie on the go! Photo and Recipe: Claire / The Slender Student
4. Chocolate and Peanut Butter Protein Pancake – 11g Protein
These are next on my list to bake. I love the winning combination of chocolate and peanut butter.
Get the recipe from The Lemon Bowl
I hope these have inspired you to give them a try?! There are so many different recipes I'm sure you'll find one that you all love and remember these aren't just for breakfast. Awesome as a post workout snack too!

subset: commoncrawl


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Available in:Canada
NetEnt are known for extravagant games, but it was an old favourite that made the headlines after a Norwegian player won big. Mr. Nilson from Oslo was perusing the provider's slot range on Leo Vegas Casino and decided to have a punt on Tiki Wonders: a NetEnt slot that's been overlooked recently in favour of the fancy 3D graphics of recent releases like Gonzo's Quest and Jack and the Beanstalk. However, the move paid off as Mr. Nilson won the big progressive jackpot, netting €830,000.
The main lesson for shrewd spinners to learn from Mr. Nilson's luck is that it's about how the slot plays, not what the graphics say. And Tiki Wonders remains a top drawer progressive jackpot slot. The overall feel of the game is unspectacular, but still pleasant, functional and fun. The Hawaiian vibe is gently done, with a beach background, the sound of lapping waves and beach holiday-themed icons ranging from dolphins to daiquiris.
With players able to play between one and 30 winlines, wagering £0.01 to £0.50 per line, the game offers a lot of flexibility in how it's played. The standard jackpot pays 10,000 coins and there are two progressive jackpots up for grabs: the Wonder Jackpot, which stood at £4,200 at the time of writing, and the Mega Wonder Jackpot, £16,811. So there's serious cash up for grabs.
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by Lisa
(Greentown PA)
Almighty God Heavenly Father I come to you today asking for your help in saving my marriage from divorce. Please help us both to heal from the hurt and pain we caused each other. Lets learn to forgive and begin to love each other as strong as we did before our troubles started. Helps us raise our children together in a loving and happy home. I know this is possible with your help and guidance. I pray to you Heavenly Father to show us the way.
Join in and write your own page! It's easy to do. How? Simply click here to return to Daily Prayers.
Click for Inspiration
50 Inspirational Bible Verse Pictures
See What Happens After A Single Act Of Kindness
By The End of This Video, Your Faith Will Be Stronger Than Ever
See What Happens When Someone Pays For A Random Stranger's Groceries
Popular Prayers
Must Reads:
5 Powerful Prayers for Prosperity
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8 Prayers You Need to Know by Heart
The Trick to Powerful Prayer


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Product Information
Product Detail
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- Package Details : Saree with Matching Blouse Piece
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Temporary Employment Service
The Temporary Employment Service (TES), operated by Carnegie Mellon's Human Resources, is dedicated to providing qualified temporary office support staff quickly. TES understands your needs and retains a pool of qualified clerical, secretarial, and administrative staff who are available for full- or part-time, short- or long-term assignments at the university.
TES employees are eligible for university benefits based on their hours and length of employment, as with other full- and part-time employees of the university. All employees are required to work the minimum hours necessary to maintain benefit-eligibility status. Changes to hours or gaps in employment may result in an interruption of university benefits or a change to the benefit eligibility status.
If you have questions about TES, please contact Pat Schaller, Manager of TES, at email@example.com or 412-268-7478.
To request a TES employee:
Contact TES to discuss your department's hiring needs.
Create a Position in Workday and choose 'Fill via TES.'
Result: TES reviews and approves the position in Workday.
Initiate Add Job once position approved to hire TES employee into the position
For information about how to create positions through Workday, review the "Add Additional Job Quick Guide" in the My Workday Toolkit.
Hiring Manager Information
When your temporary employee arrives:
- Provide a designated work space and computer.
- Orient the employee around the office, and introduce them to others with whom they will be working.
- Establish any alternate work schedules that may be necessary. TES employees are aware of the university's normal university business hours, and the possibility that different hours may be required. However, you should inform them of the specific schedule they should keep, including break periods. Note that TES employees are entitled to overtime pay for hours worked in excess of 40 per week, under the Fair Labor Standards Act.


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Remodeling Services
REMODELING PROJECT IN MIND? 7 Years Experienced Handyman Contractor! Interior and Exterior Painting, popcorn removal, texture, lights and fans install, laminate and Tile floors installation.
Dirty exterior or driveway? We also do pressure wash, Window clean and much more!
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CALL US FOR A FREE ESTIMATE! Mike or Alex


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We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
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It's more than just the Chicago-set sitcom that introduced us to
Christina Applegate. It might just be the key to happiness. Sound
Let's backtrack:
We all assume kids make us happy. We remember birthday parties
and piano recitals and afternoons in the park. And if memories
fade, there are always home movies and bronzed baby shoes and hard
drives full of pictures to remind us that parenthood is an
unparalleled joy and privilege.
And yet for years, research has repeatedly told us that the opposite is true,
that having kids actually decreases a married couple's happiness.
Fewer nights out with the gals, fewer tee times with the guys,
stress about babyproofing and bills and bullies -- it all adds us.
Not that it stopped anyone from having kids, but nearly all the
existing research pointed to one reality: Having kids makes parents
less happy.
But an news tidbit out of Scotland last week has added something
new to the debate.
An article published last week in the Journal
of Happiness Studies (to which I'm now seriously considering a
subscription) says that having kids makes a married couple happier.
Combing through 15 years of data from the biennial British
Household Panel Survey, researchers found that:
Contrary to much of the literature, our results are consistent
with an effect of children on life satisfaction that is positive,
large and increasing in the number of children.
That last part? It means the more kids, the better -- up to
three. (Kind of makes you wonder what it is about that fourth
It's also worth noting that for single people, kids decreased
happiness. Most (though not all) single parents did not plan on
going it alone, and as anyone with kids knows, it's a pretty heavy
load to bear.
No survey will change the fact that most of us want to have
kids. Chalk it up to instinct, social norms, or one too many
Lifetime movies -- we're wired for family. The birthday parties and
piano recitals and afternoons in the park cloud over the terrible
two's and chicken pox and the specter of college tuition costs.
(For a parent's take on what it's all worth, check out Walter
B's Oct. 13 post,
What is a parent's return on investment?)
Let us plan your weekend with the best family events and activities in Chicagoland.
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It is all PlayStation this week as the guys gear up for the new consoles.
Ken McKown
Jason Gambrel
Ryan Wombold
Drew Leachman
John Whitehouse
Games Covered:
Call of Duty: Ghosts
PlayStation History
And more…
N4G is a community of gamers posting and discussing the latest game news. It's part of NewsBoiler, a network of social news sites covering today's pop culture.


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Mr. Ilya Kurylev
Moscow - Russia
No approved endorsements
About Mr. Ilya Kurylev
I'm involved in gaming industry since 2003.
Took part as concept or game designer more that 45 mobile, PC and Web game projects in cooperation with 6 game development studios.
Current location: Moscow (Russia)
My major specialization is game desing for online & casual markets.
My field of activity:
- game design for casual games
- browser-based MMO games and online fun services
- psychological analysis and adaptation of games for casual market
- adapting video games to mobile or flash games, advertising games
- using brands and licenses for game design.
My experience on this field:
- adaptation of fantasy novel of famous Russian fiction writers Sergei Lukyanenko and Nick Perumov for RPG-game "No Time for Dragons" (PC)
- football manager game licensed by famous football club (not announced yet, Mobile)
- design fo casual & fun pool game (Pool Arena, J2Me)
- set of casual erotic arcade games (Mobile)
- free online flash games (www.gametop.com)
- browser-based MMO social game (in production)
Feel free to contact me for consulting or something else.
E-mail: firstname.lastname@example.com
Portfolio and blog (rus): http://igrostroy.ru
Profile (eng): http://www.linkedin.com/in/prospero
Profile (rus): http://iprospero.moikrug.ru
If you notice offensive or incorrect information, please contact us.
Mr Mike Fedorov
Executive producer@


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Author has written 9 stories for Dragon Ball Z, DC Superheroes, and Transformers/Beast Wars.
Hi, I don't update this often enough to stick much up. I'm no longer in my twenties (gosh, how time flies) and I like comics, the odd anime, exercise (sometimes), and Transformers. I come from Australia.
Thanks to Anja for helping me come up with the concepts for Legends of Kakarott. It is on my list to finish. Well, not to the point I was originally going to take it, but to a better spot then it currently is.


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February 14, 2011
Photo by Seth Browarnik for WorldRedEye.com
Pitbull stopped by the Fountainebleau Hotel last week while the Elvis Duran show was in town and doing a live broadcast from the hotel. Too many quotables in this interview. Way too many. You gotta listen to this interview. Listen below…
Pitbull Interview with Elvis Duran Morning Show
Download: Click Here
CategoriesMedia WatchMusic


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College Division
College Division:
- Conducting
Pre-College Division
Pre-College Division:
- Chorus
Contact Information
Contact Information
Born in Johnstown, PA
Choral director at the Riverdale Country School, Sarah Lawrence College, and Perlman Music Program. Performances with renowned ensembles and orchestras, including the American Bach Soloists, San Francisco; Bethlehem Bach Choir and Orchestra; Paul Hill Chorale at the Kennedy Center and the Smithsonian Chamber Players, Washington D.C.; Canticum Novum Chamber Ensemble at Merkin Hall and Alice Tully Hall, N.Y.C. Festival performances include the Pablo Casals Music Festival in San Juan, P.R.; Madeira Bach Festival, Portugal; Marlboro Music Festival, Vermont; and U. of Maryland Handel Festival. Recordings include Bach's B-Minor Mass, American Bach Soloists; The Christmas Story, the Waverly Consort; Mozart Requiem, Amor Artis Orchestra and Chorus; Bach's St. John Passion, Smithsonian Chamber Players; and Tippicanoe and Tyler Too, a collection of campaign songs from the early 1920s and '30s. Distinguished Alumnus Award in 2001, School of Music, West Chester U. Current faculty, the Riverdale Country School, Sarah Lawrence University, and Perlman Music Summer Program.
Degrees and Studies
B.M., M.M., West Chester U., in vocal performance and choral conducting.


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Someone once told me that starting a company is a lot like owning a boat. The best two days are the day you buy it or start it and the day you sell it. So today is a good day for StillSecure, which just announced that it has sold its managed security services business to SilverSky.
I helped my long-time friend Rajat Bhargava start StillSecure back in the dark days after 9/11. Over the years, the company went through many twists and turns. We built an intrusion prevention product and vulnerability management platform, pioneered network access control, and finally acquired and grew a managed security services business.
I left StillSecure in October of 2009 but have stayed close to my friends there. I have watched as they continue to grow and move the company forward. So I was very happy to see the MSSP division find a long-term home with SilverSky.
I, of course, am very familiar with SilverSky, having covered them here in Network World, as well as on my own blog. I spoke with SilverSky CTO Andy Jaquith about this acquisition earlier this week. Andy and the SilverSky team are very excited acquiring the StillSecure MSSP business. They get about 40 great people to join the company, including the SOC/NOC staff here in Florida, the MSSP sales team, and an engineer or two in Colorado.
SilverSky also inherits the great partner/channel relationships that were built over the years, as StillSecure concentrated on the hosting provider market for its MSSP business. Nurturing those will be the key to maximizing the value of this acquisition. SilverSky has some great security services and will add the StillSecure offerings to the mix. Andy says they are still working out what parts of the StillSecure information management infrastructure they will use long term. For now, everything will remain the same.
SilverSky's MSSP business is pretty big and the StillSecure MSSP business is not going to be a huge add to the revenue. But the people and resources should prove very valuable to SilverSky.
Details around financials of the transaction were not disclosed and I am not going to speculate here. I have always been a big believer in the MSSP market and think that this will result in a stronger SilverSky.
What about the non-MSSP part of StillSecure? At this point, it is the network access control business. Safe Access has always been a quality product in the space. It has enjoyed wide adoption within the U.S. DoD and in private enterprise. StillSecure will continue to exist as a NAC provider. Former CTO James Brown will step in as CEO of StillSecure and lead the company. Raj Bhargava will stay on as Chairman, but is involved in several other new ventures. Other StillSecure executives will be leaving to pursue new opportunities. It will be interesting to see how the NAC business does set free of the MSSP and other business priorities.
In the meantime, I am happy to see this new development in the StillSecure lifecycle. I think back to all of the great people I have worked with and met at StillSecure. It gave me a chance to become part of the great community of security people that I still interact with every day. That really has been the greatest part of being involved with the company. I hope the MSSP business continues to thrive with SilverSky and the remaining Stillsecure business also continues to seek out new opportunities and new business.


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"Obesity is a disease. It is not a lifestyle choice, from a lack of willpower or personal weakness. Obesity is a medical condition that should be treated from a medical perspective."
-Dr. Scott Isaacs
About Dr. Isaacs
Scott D. Isaacs, M.D., F.A.C.P., F.A.C.E. is a board-certified endocrinologist, obesity medicine specialist and medical director at Atlanta Endocrine Associates. He attended medical school at Emory University School of Medicine and continued on for residency and fellowship in Endocrinology, Lipids, Diabetes and Metabolism.
He currently serves as a clinical faculty member at Emory University School of Medicine. Since 1998, Dr. Isaacs has focused his medical practice on endocrinology and medical weight loss. Dr. Isaacs has published research on diabetes, thyroid and obesity in medical journals. A frequent speaker to national and international groups, he is a Diplomate of the American College of Physicians and American Board of Obesity Medicine and a Fellow of the American College of Physicians and the American College of Endocrinology.
Dr. Isaacs is past president of the Georgia Chapter of the American Association of Clinical Endocrinologists (AACE) and currently serves AACE on a national level. Dr. Isaacs is past president of the Atlanta Chapter of the Atlanta Diabetes Association. Dr. Isaacs is an expert contributor for ABC, CNN, NPR, numerous national magazines, newspapers, websites and television shows.
Dr. Isaacs lives with his wife and daughter in Atlanta. He enjoys taking spin classes, hiking, biking and learning Spanish.
Dr. Isaacs is the author of several best-selling books on hormones and weight loss including:
- Hormonal Balance: How to Lose Weight by Understanding Your Hormones and Metabolism
- The Leptin Boost Diet
- Beat Overeating Now! Take Control of Your Hunger Hormones to Lose Weight Fast
- Overcoming Metabolic Syndrome
- A Simple Guide to Thyroid Disorders


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Alina – Cute teen girl looks so sexy with boyfriend finally fucking her
Released: October 6, 2014
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text:
The extension of the Sun brand to cable news led, according to Quebecor insiders, to the most expensive specialty channel launch in Canadian history last April. Critics have panned the network as tasteless, if not un-Canadian. But Quebecor insists Sun News "regularly" beats CBC News Network and CTV News Channel in prime time.
It is also losing buckets of money. In the third quarter this year, operating profits in Quebecor's broadcasting division plummeted 77 per cent, primarily due to the launch of Sun News. But Quebecor has made a five-year commitment to Sun News, giving the network time to prove itself.
Mr. Péladeau seems sold on its convergence potential. Of course, weakening the CBC would make it all so much easier, not just for Sun News, but for all of Quebecor's broadcasting and Internet assets.
Mr. Péladeau insists the frequency with which Sun Media's newspapers have filed access requests regarding the CBC in recent years is in keeping with their long tradition of holding public agencies and officials to account.
The debate about the CBC's mission is as old as the public broadcaster, which turned 75 this year. But Mr. Péladeau argues it has never been as relevant as in the current multi-channel, multi-platform universe.
"I listen to the news on Radio-Canada everyday. As a citizen, I think it is undeniable that it has a news mission," Mr. Péladeau offers. "But should Hockey Night in Canada be on the CBC? Should Jeopardy! be on the CBC?"
Mr. Péladeau seized on the Nov. 24 testimony before the House of Commons Ethics Committee, which oversees the Access to Information legislation, of CBC general counsel Maryse Bertrand to show that public network competes directly with private broadcasters, instead of complementing their activities.
Asked why the CBC refused to make the salaries of Mr. Mansbridge and other network stars public, Ms. Bertrand responded: "It's the war for talent, basically. If precise salaries for our talent or even our programs are known, then others can try to poach them or somehow compete more efficiently against us than they would otherwise be able to."
There is not much sympathy for Mr. Péladeau's crusade in Quebec, where Radio-Canada remains a cherished cultural institution and, unlike its English-language arm, a ratings success story. Any diminution of its budget, the thinking in Quebec goes, would only enhance Quebecor's domination of the province's media landscape.
Last month, Enquête, a Radio-Canada version of The Fifth Estate, ran a highly critical probe of the "Quebecor Empire" that suggested Quebecor routinely advances its corporate interests in its newspapers and on its TV channels.
The program included a segment on the illegal hacking scandal facing Mr. Murdoch's News Corp. in Britain, noting that politicians there criticize the Australian-born magnate and his media properties at their peril.
Mr. Péladeau dismisses the Enquête segment as "intellectually dishonest" and a form of "guilt by association" timed to coincide with CBC president and CEO Hubert Lacroix's muscular counterattack against Quebecor. According to Mr. Lacroix, Quebecor has received more than $500-million in direct and indirect federal subsidies in the past three years, including favourable auction conditions for the wireless spectrum its Vidéotron subsidiary needed to enter the lucrative cellphone business.
The charge that politicians cower before Quebecor stems not only from the spectrum auction. It results from the Quebec National Assembly's passage this fall of a law that forbids legal challenges to a contract granting Quebecor exclusive lease and naming rights on a new major league hockey arena planned for Quebec City.
Mr. Péladeau counters that the law was adopted at the insistence not of Quebecor but of Quebec City Mayor Régis Labeaume, who is seeking to resuscitate the Nordiques franchise and thinks Quebecor would be the best owner. So, says Mr. Péladeau, does the NHL.
"We've made representations to the league. They like our business model," he says, alluding to the convergence strategy Quebecor intends to employ by broadcasting Nordiques games on its new TVA Sports channel and Vidéotron's smartphones. Indeed, it is the same model that inspired the $1.32-billion purchase this month of the owner of the Toronto Maple Leafs and Raptors, by BCE Inc. and Rogers Communications Inc.
The prospect of a Quebecor-owned hockey team sends shivers up the spines of media watchdogs in Quebec, who fear it will only increase the company's dominance of the province's media market and turn its news outlets into Nordiques cheerleaders.
Mr. Péladeau sees it differently. Quebecor Media is 45-per-cent-owned by the Caisse de depôt et placement du Québec, which manages the assets of the Quebec Pension Plan. Hence, what's good for Quebecor is good for Quebec.
Or, as he puts it: "Working for Quebecor is like working for all of Quebec."
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Prestigious villa built in a Mediterranean style, facing east with a total built of 489m²in 2 levels plus terraces and porch built on 2 fantastic 3084 m² plots. This property is located in one of the best area of sotogrande Costa, near Royal Golf course with views to the pool and garden. It has built with high qualities, offers 7 bedrooms in total, 5 bathrooms en suite, huge living room into 2 levels with fireplace leads out to a porch, separate dining room, large fully fitted kitchen with exterior breakfast area. Laundry and maid room en suite with storage. The house enjoys a fabulous matured garden with a pergola in a low level with BBQ opens to the garden and the pool and a nice solarium. This generous property also has a guest house with 2 bedrooms, 1 bathroom. Marble floors throughout the house, air condition, and a magnificent mature garden. Ideal house in a beautiful and quiet street of Sotogrande.
By turns, Costa del Sol is uniquely Andalusian, classically Spanish and altogether international. At its most expansive definition, Costa del Sol, the sunshine coast, stretches 185 miles or so, from La Herradura on the easternmost tip of the province of Málaga to Gibraltar on the western border.Costa Del Sol 소재 최고급 주택들에 대해 자세히 알아보기


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Garden Pond>
Pond Medikations>
JBL ArguPond
Price including german VAT plus shipping costs
JBL ArguPond is a remedy for ornamental fish in the garden pond against carp lice (Argulus), gill lice (Ergasilus), anchor worms (Lernaea) and other parasitic crustacea. Fish infected with carp lice are shy, show abnormal behaviour -particularly leaping at night- and try to rub against stones. Carp lice are visible to the naked eye as 4-12 mm long parasites, shaped like a finger nail. Gill lice cause widespread damage to the gill epithelium. The fish show signs of difficulty with respiration, become weak and lose weight. Gill lice are about 1.5 mm and can be seen on the gills with the naked eye. Anchor worms can be clearly identified as long, rod-shaped or branching parasites with two long egg sacks. The head burrows into the scale pockets, causing inflammation.
Application and dosage JBL ArguPond:
40 ml to 450-500 litres of garden pond water. Dwell time in the pond one week. Change 30% of the water afterwards. Treatment can be repeated after two weeks if required. It is recommended to use JBL AccliPond for regeneration of the sensitive mucous membrane.
500 ml are sufficient for 6000 of pond water.
Do you have questions about this article ?
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Acoustica Mixcraft Pro Studio 7
Mixcraft Pro Studio 7 is a powerful music production and multi-track recording workstation with over 7000 music loops and dozens of audio effects and virtual instruments. Record audio, arrange loops, remix tracks, score and edit video, and mix and master tracks to create polished, professional compositions. >More
Copyright ©2017. This site is protected by copyright. There are terms for its use. Send us Feedback on our site and your experiences. Our descriptions, pictures, and prices are mostly accurate, but cannot be guaranteed. Cookies should be enabled in your web browser for full site functionality (details).


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
what is a trust account and how does it work?I have some one in family member that is a trustee, Went father died. And land came on sale and it was sold, but the trustee is one of the family member holding the fund by is trust account, for him to hold it for longtime before it spit to the other family member. Can he hold it out for a longtime with out giving it to the rest of the family. yes or no thank you from Loretta
A trust account is, generally speaking, an account that is held by a trustee on behalf of a beneficiary. Specifically, attorneys hold client funds on behalf of clients. When an attorney earns fees, the attorney, after the client reviews and approves the attorney's bill, transfers client funds from the trust account to the attorney's general account as fee payment of fees.Ask a similar question
A trust account in its broadest sense is not necessarily handled by an attorney. Numerous independent trust companies and banks have trust departments that hold assets in trust for beneficiaries. How the accounts work depend greatly on the documents that create the account. For example a trust account that is for a trust containing the term "My wayward son Johnny only has access to this money for issues concerning his health, education, maintenance and support. The trustee has full authory to independently determine the validity of any of Johnny's requests" is not likely to work by giving Johnny a debit card on the trust account.
Rules about how attorney trust accounts work are found in the Rules of Professional Conduct 1.15 a and 1.15b. See the attached links for a full description.Ask a similar question
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Overview Edit
U.S. copyright law Edit
If the copyrighted work and the defendant's work are strikingly similar, the first element of access may be assumed without proof (at least in civil copyright cases), especially when the copyrighted work was widely available.[1]
- ↑ See, e.g., Playboy Enters., Inc. v. Frena, 839 F. Supp. 1552, 1556 (M.D. Fla. 1993) (full-text) (holding proof of access unnecessary when defendant made "essentially exact" copies of copyrighted photos that appeared in nationally circulated magazine).


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16592 Westway Drive, New Buffalo 49117
See more homes for sale in New Buffalo, MI
This property has been Sold!
07/08/2016 at $2,895,000
Coastal casual luxury is defined in this 4BR/4.5BA, light-filled lakefront home in Timber Lane Assn. Open floor plan with gorgeous Lake Michigan views. Eat-in kitchen and dining area with fireplace lead to large screen porch overlooking the lake that has retractable windows for comfort in 3 seasons. Main floor master has beautiful custom bath, large walk-in closets, opens to private deck. Spacious great room has a stone fireplace for cool nights. Upstairs is second master suite with private bath, and 2 more bedrooms. Follow the stone path out to sitting area on the bluff and watch the sunset! Plenty of room for a pool and has 3-car garage. Timber Lane is close to everything but feels far from it all! This may be the Harbor Country getaway you've been dreaming of!
Property Information
- Status
- Sold
- Address
- 16592 Westway Drive
- City
- New Buffalo
- Zip
- 49117
- County
- Berrien
- Township
- New Buffalo Twp
- Total Finished SqFt
- 4,700
- Above Grade SqFt
- 4,700
- Garage Desc.
- 3, Attached
- Waterview
- Waterfront
- Waterfront Desc
- All Sports, Assoc Access
- Waterfrontage
- 102.0
- Body of Water
- Lake Michigan
- Water
- Public
- Sewer
- Public
- Year Built
- 1989
- Taxes
- $18,538
Rooms and Land
- Basement
- Partial Basement
- Heating
- Forced Air, Natural Gas
- Acreage
- 0.64
- Lot Dimensions
- 275.75x99.94
- Features
- Security System
- Exterior Materials
- Wood
Listing Video for 16592 Westway Drive, New Buffalo MI 49117
Mortgage Calculator
Listing Broker
Listing Courtesy of Coldwell Banker Residential Brokerage
Office Address 10 N. Whittaker St.
Listing Agent Chad Gradowski
Listings last updated: . Some properties that appear for sale on this web site may subsequently have been sold and may no longer be available.
Our Michigan real estate agents can answer all of your questions about 16592 Westway Drive, New Buffalo MI 49117. Real Estate One, Max Broock Realtors, and J&J Realtors are part of the Real Estate One Family of Companies and dominate the New Buffalo, Michigan real estate market. To sell or buy a home in New Buffalo, Michigan, contact our real estate agents as we know the New Buffalo, Michigan real estate market better than anyone with over 100 years of experience in New Buffalo, Michigan real estate for sale.
The data relating to real estate for sale on this web site appears in part from the IDX programs of our Multiple Listing Services. Real Estate listings held by brokerage firms other than Real Estate One includes the name and address of the listing broker where available.
IDX information is provided exclusively for consumers personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing.
All information deemed materially reliable but not guaranteed. Interested parties are encouraged to verify all information. Copyright© 2017 MichRIC LLC, All rights reserved.


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Incredibly, Samsung is the only smartphone maker who tests smartphone batteries in its in-house test labs in the United States where all other smartphone makers, including Apple, get their phone batteries tested at any of 28 test labs certified by the American Cellular Telecommunications Industry Association. Even though Samsung's in-house lab has been approved by the CTIA, the question remains whether Samsung overlooked test results in a rush to release the Galaxy Note 7 in global markets.
Samsung's haste in launching Galaxy Note 7 may have resulted in battery woes
The CTIA-mandated tests on smartphone batteries revolve around battery performance when calls are in progress, when devices are being charged and also when the external temperature rises suddenly, like during the peak of summer. Once a battery clears all these tests, only then is it considered safe to be shipped with smartphones. In the case with Galaxy Note 7, there have so far been 96 reports of overheating batteries, 13 reports of them burning up and another 47 of them causing property damage. So many issues with a particular smartphone battery type certainly raises questions about the quality of testing done in the first place.
Reportedly, Samsung is still clueless about why the battery issues occurred, even though several theories about the same have been floated by company executives and third party experts alike. A number of such theories have pointed to the fact that Samsung observed incredible haste in rolling out Galaxy Note 7 in global markets, probably with an eye on Apple iPhone 7 Plus releasing in September.
Samsung Galaxy Note 7 battery woes a result of high capacity Lithium batteries
Last month, a report in Bloomberg explained how Samsung shifted the initial launch date of the Galaxy Note 7 from September to mid-August and then to August 3. Bloomberg said that Samsung's employees worked overtime and slept at their desks to save time and to ensure that the phablets could reach the world a month before Apple's iPhone 7 Plus did. It also added that suppliers were put under intense pressure as Samsung changed the specifications of the upcoming phablet time and again. We believe a lack of initial planning and too much haste resulted in batteries not working too well with the final version of the handsets.
After the battery fiasco became too much for the company to handle, the Chairman of the U.S. Consumer Product Safety Commission stated that batteries in Galaxy Note 7 handsets short-circuited as they were too big for their compartments and the lack of space caused undue pressure during charging. We wonder if the batteries would have cleared stringent testing had they been subjected to testing at any of the 28 labs approved by the CTIA rather than being tested in-house by Samsung. It will also be interesting to see if Samsung will follow the same procedure with its upcoming phones like the Galaxy S8 and Galaxy S8 Edge which may release in February next year.
Source: Wall Street Journal


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Wire wrapped velvet headband. Malleable to fit any look or do!
Velvet Wire Wrap Headband styled by the FP Me community
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When you purchase a digital subscription to Cake Central Magazine, you will get an instant and automatic download of the most recent issue.
Three tier, square, vanilla cake with Italian meringue buttercream, covered in ivory mmf. Purple ribbon and real hydrangea on top.
Purple Paradise
First Wedding Cake
Red And White Square Wedding Cake


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Dakota Fanning Gets Her Pilates On (PHOTOS)
Dakota Fanning may be a mere girl of 16, but clearly she understands that it's never too early to develop a proper exercise regimen.
The New Moon actress, who's been known to bust out some cheerleading routines for her high school when she's not on the set, reaffirmed her commitment to fitness on Friday by attending an early-morning Pilates class. Looks like she worked up quite the sweat while she was in there, too.
Was it D-Fan's most glamorous moment? Perhaps not. But her habits appear to be much healthier than those of her castmates.
Let's all celebrate Dakota's health-consciousness by clicking through the photo gallery, shall we? And don't forget to hit up The Forks Report for all things Twilight-related.


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- Description
These 95mm outside diameter split tube rings, specifically designed for the Takahashi FS-78 and Sky 90, are made of die-cast aluminum, finished in gloss black paint, and lined with felt. The hinges, no-tool knobs, and hardware are made of stainless steel for long life and easy telescope installation and removal.
Parallax Instruments Standard Telescope Mounting Rings for 95mm OD Tubes
The rings are installed onto your mount or mount dovetail plate via a 1/4"-20 thread mounting hole that is drilled and tapped in the flat boss on the bottom of each ring. A similar hole is drilled and tapped on the top boss so that accessories, such as a piggyback camera mount, can be attached.
Parallax Instruments Product Number: 95MM
When you get to your shopping cart, please write what you are going to mount the rings to in the comments section. Most standard rings are in stock, however, certain mounting set-ups may require some custom drilling and thus shipping may take a little longer. Please call us at 1 (800) 483-6287 or email us with any questions. Thanks!
- Additional Information
Additional Information
SKU PA-95MM Manufacturer Parallax Warranty Not Supplied By Manufacturer
- Reviews
- Included Items
Included Items
- Parallax Instruments Standard Telescope Mounting Rings for 95mm OD Tubes
- Hardware
- Questions & Answers
Product QuestionsQuestion by: bob spaulding on Aug 2, 2015 6:39:00 AM
- Support / Downloads


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Blending vibrant surroundings, a host of facilities and an inviting ambience, this superb property offers the ideal backdrop for a family holiday. The family-friendly business hotel offers 50 guest rooms in total and it is fully air-conditioned. Facilities available to guests at the casino resort include a lobby with 24-hour reception and check-out services, a casino, caf�, bar, pub and breakfast/dining room. The beach hotel features 2 meeting rooms with conference facilities, and wireless Internet access is available. Guests arriving at the themed hotel by car may leave their vehicle in the car park.
This hotel is located in the centre of Gruissan, set in a lush environment of striking natural beauty with a lovely pond. Guests will find links to the public transport network and the casino in the immediate vicinity, while the shops and restaurants are around a 5-minute walk away. The nightlife of Gruissan is around 10 minutes' walk away and the port and nearest beach are roughly 20 minutes on foot. Narbonne, with its cathedral and train station, is approximately 13 km from the hotel.
All rooms are equipped with a minibar, hairdryer, safe and individually regulated air conditioning and heating. An en suite bathroom with bathtub, a double or king-size bed, a direct dial telephone, ironing set, satellite TV, Internet access and a private balcony or terrace also feature as standard.
There is an outdoor swimming pool with children's swimming area. Sun loungers and parasols are provided by the pool and on the sandy beach.
A breakfast buffet is served each morning and set menu and � la carte options are available for lunch and dinner.
American Express, Diners Club, MasterCard and VISA are accepted as methods of payment.
Room Facilities:
Direct dial telephone
Satellite cable TV
Internet access
Iron and board
Double bed
King size bed
Individual airconditioned
Individual heating


text: Posted December 9, 2012 No matter what Rivera should be fired. He's already proven in my books he's not HC material... at least not for the Panthers. And to an extent I agree, however is there really any other slam dunk candidates out there. Chip Kelly is high risk/high reward. I would definately fire him for a Gruden or Cowher. But do we really roll the dice on another hot coordinator? Again, im not really a huge fan of Rivera but running the table and going 7-9 atleast warrants re consideration, especially after beating the Falcons. Share this post Link to post Share on other sites


text: Mercy Johnson returns to movies, flaunts curves on set (Photos) The actress who gave birth to her third child in December 2015, showed off her curves on set of a new movie..Safe to say she's back! See another picture below: ALSO READ: Adorable! Mercy Johnson rides bicycle with her Kids | Peek 06:33 Entertainment, featured, Mercy Johnson, Nollywood


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Lilly & Sid Retro Dino Dungaree Set
£16.00 £28.00
Dinosaurs and stripes- a unique outfit for baby boys this season. The zipper front dungarees are made from lightweight sweatshirt fabric with Dino Print design and jersey cuff hems. The...
we will always endeavour to send your goods in the next post available. at the very least, items ordered before 5pm will be posted the next working day.
you can choose between 1st class which normally takes 1-2 working days or 2nd class which normally takes 3-4 working days. the cost is £2.99 (1st class) or £1.99 (2nd class). international postage is also available via royal mail which comes out at a cost of £9.50 (europe) or £10.95 (worldwide).
we reserve the right to use an alternative courier for selected larger items. this will not impact the cost, or time scales of delivery.
Express Shipping
if you are in a hurry to get your goodies (excited!) an express shipping service is available on all products ordered before 3pm, at a cost of £6.95.


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Hi all,
We are migrating to a new XenApp 6.5 with 6 servers.
My question is, how do you manage these XenApp servers for maintenance? Like when you want to test a new published application?
At the moment, Im doing this:
- Disable logons on Server1 (for example).
- Install the published application, publish it to server1
- Quickly enable the logins on Server1. Start the new published application
- Disable back the logons to Server1, so nobody can logon to this server.
Whats the best procedure for this?
2 Replies
Dec 7, 2011 at 6:49 UTC
I have 6 Xenapp servers, for testing I have a 7th that doesn't associate with the farm. From there I will publish a new app and test myself. If the app is specific I will get a test bed of users and assign them to that server, then give them a unique login for that specific Citrix test server. After testing the app will be published to all the rest and normal logon resumes.
Dec 7, 2011 at 7:35 UTC
Our test server is in the farm, but the applications available to standard users don't run on it. A separate set of apps that run exclusively on that server are set up for admins to use. If we need a wider testing group, we'll push some users onto the test server temporarily by publishing the test server apps to those users.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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Home / apparel / men's apparel / men's clothing / men's jeans /
Men's Jeans
Levi Men's 501 Denim Jeans
4.4 Based on 199 reviews
reviews (199)
shipping & returns
additional info
Product information
Since Levi's invented it in 1873, the 501 Jean has been a blank canvas for self-expression. True to the original, it features the time-tested straight fit and our signature button fly with branded Levi's shanks. This pair comes in a medium stonewash. Stonewashing is accomplished by washing denim with pumice stones to achieve a lived-in look and feel. This step occurs during the finishing process.
- 100% Cotton
- Regular fit through seat and thigh
- Mid rise - 11.25" front rise
- Straight leg - 16" opening
- Measurements based on size 32
- Non-Stretch Denim
- Button fly
- Imported


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Top 5 Things to Do This Weekend
See also:New Times' Calendar of Things to Do See also: Five Must-See Summer Olympics Parties See also: Five Favorite Bowling Alleys in Phoenix
If you tear yourself away from watching Olympic events this weekend (you know you're stoked for the trampoline and race walking events) or playing flip-cup in honor of them, here are your best bets for a good time.
Scream Screening @ FilmBar Becky B brings Wes Craven's Scream and all its '90s camp to FilmBar, complete with hold the phone trivia and a blood-curdling scream war. Scary stuff starts at 11:30 p.m. on Friday. Tickets are $10.
Bobby Green, Jackson Casey, and JJ Horner will launch their new skatewear brand, Pyramid Country, atCowtown
on Saturday from 7 to 9 p.m. On display will be the sculptures and artwork associated with the brand. Organizers promise free beer and grub, as well as a contest for best trick in the shop's parking lot.
Zombie Beach Party @ Rips Ales & Cocktails Apparently, the afterlife's a beach. Embrace your undead tendencies, put on some swimwear, and get a zombie makeover at Rips on Saturday. There will be a best costume contest, drink specials (Brainy Alexanders, anyone?), a photo booth, and horror films on the TVs. Cover is $5.
Dancers And Health Together, Inc.
Arizona's Got Dance! Workshops @ Terpsicore Dance Studio Move and groove in honor of National Dance Day. Local dancers will lead workshops on styles including salsa, jazz, and krump from 9 a.m. to 5 p.m. on Sunday at Terpsicore. Admission is $10 per class or $60 for the whole day.
Flickr via Ben Husmann
Robots Pic-A-Nic @ CityScape Sunday's the day the robots will have their picnic. Dress up in droid duds for a bring-your-own-basket al fresco dinner, hosted by Downtown Phoenix Mothership, from 7 to 9 p.m. in the park at CityScape. Attendees are encouraged to bring sporting goods, blankets, and instruments, for an unplugged jamboree. The event is free and open to the public.
Related Locations
815 N. 2nd St.
Phoenix, AZ 85004
215 W. University Dr.
Tempe, AZ 85281
3045 N. 16th St.
Phoenix, AZ 85016
219 S. 5th Ave.
Phoenix, AZ 85003
1 E. Washington St.
Phoenix, AZ 85004
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Guests can enjoy the warmth of hospitality and an ideal location at this hotel. The property offers ideal hotel accommodations for anyone visiting the Princeton, in a form of beautifully appointed guestrooms, suites and Executive Floor accommodations. It offers a complimentary shuttle service to popular destinations and businesses within a 5-mile radius.The comfort of the Sweet Dreams by Doubletree plush top king bed is the centerpiece of this well-appointed room. Lie back on the five jumbo hypo-allergenic down pillows or relax on the twin-sized sofa bed and flip through the 40+ channels on the 27" TV. High-speed internet access (wireless and only $9.95 per day), task lighting, and a desktop outlet are the business amenities provided. Start the morning with a fresh cup of coffee from the in-room Wolfgang Puck Coffee Maker.
Traveling from Downtown Princeton (Nassau Street), go southeast on either Washington Road or Harrison Street to US Highway 1 and turn left. Go approximately 3 miles to Ridge Road. Hotel is on the southwest corner of US Highway 1 and Ridge Road.


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Harold Morgan
Harold Morgan is responsible for all human resources-related functions and providing strategic human resources leadership and vision. Mr. Morgan provides consultative advice to senior leadership on execution of business strategy and identify opportunities to help improve White Lodging's organizational effectiveness and growth objectives.
Mr. Morgan has spent more than 30 years working in human resources and brings the experience necessary to help White Lodging continue its pattern of strong, strategic growth.
Mr. Morgan graduated with a degree in Human Resources and Labor Economics from the Cornell University School of Industrial and Labor Relations, and is a Certified Compensation Professional. He was a member of the board of directors and search committee chairman for the International Heath and Racket Sports Association, a member of the Organization Resources Counselors, a member of the Human Resources Policy Association and a member of the Machinery and Allied Products Institute.


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Italian coachbuilder Touring Superleggera has something up its sleeve for this year's Geneva Motor Show, and this is most likely the front of that car.
The 2013 Disco Volante is based on the Alfa Romeo 8C Competizione, and seems to be using the Ferrari 599's headlamps. Like if the 8C's body wasn't pretty enough...
Enjoy the sexiness.


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Home Climate Control Remote
DIY Zoning Project
- July 17, 2015
( 9)
Create as many climates in your home as you never thought imaginable. Control temperature, humidity and ventilation. Do it on the schedule. Have everything measured and recorded. Do it from anywhere.
This is a remote control for Home Climate Control system.
Requires DIY Zoning software and hardware installed.
Read more at http://www.homeclimatecontrol.com/android
This is a remote control for Home Climate Control system.
Requires DIY Zoning software and hardware installed.
Read more at http://www.homeclimatecontrol.com/android
Easy to use with the proper hardware. Automatically sets temperature in various zones depending on time of day and schedule. Able to change temperature setpoint temporarily/indefinitely in case you have guests/want a different temperature than usual.
Marmaduke31 or superfreak78@22
Excellent app for controlling home climate An indispensable companion to DIY Zoning software (DZ3) allowing assessment and control of your home environment from anywhere your phone has a data connection. Intuitive and informative app that my (young) kids can work out in less than 2min. Run HVAC, a boiler or other mechanical climate control devices? Then get DZ3 deployed and take control of how your home feels with the help of this app
What's New
r150717: bugfix release
r1417: multiple updates related to landscape orientation and big screen displays, stability, responsiveness
r1239: stability improvements
r1233: "return to schedule" now implies "take off hold"
r1228: long press on setpoint button will now return settings to schedule
r1225: period name displayed in stikethrough if actual settings differ from scheduled
r1223: now displaying schedule period name


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University of Minnesota students to rally at capitol today
ST. PAUL, Minn. (AP) -- More than 300 University of Minnesota students from throughout the state are scheduled to rally today in the capitol to drum up support for higher education funding.
The rally is scheduled for noon in the state Capitol Rotunda. Gov. Mark Dayton, U.S Rep. Keith Ellison, state Sen. David Senjem and university President Robert Bruininks are also scheduled to attend.
The Minnesota Student Association is hosting the event. MSA President Sarah Shook says the group wants to make sure the Legislature considers the university and its students as it seeks to fill a $6 billion deficit.
Separately, Bruininks is scheduled to testify before the House Higher Education Policy and Finance Committee today about the impact of proposed budgets cuts on the university and its students.


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Long lasting and latex safe formula that is glycerine and fragrance free. The shooter is perfectly designed for easy anal insertion, with just the right amount of lube. With a wealth of nerve endings, stimulation of the anus can be a very pleasurable experience for you and your partner.
Delivery & Returns
Additional Information
|SKU / Product Code


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Fire crews control mattress factory fire
Fire crews have controlled a blaze at a bedding factory in Melbourne's northern suburbs.
The fire broke out in High Street, Epping, just before 9:30am (AEST).
Metropolitan Fire Brigade (MFB) assistant chief fire officer, Rob Purcell, says there were concerns about a toxic plume of black smoke.
"We've got a scene here with a mattress factory, not particularly large but given the contents, polyurethanes and foams created quite a large plume of black toxic smoke," he told ABC local radio.
Residents in the area bounded by High Street from Jovic Road to Deveny Road have been advised to close their windows, turn off their air conditioners and shut their doors.
He says there were also concerns about the smoke being sucked up by the air conditioning system at the Northern Hospital.
Assistant chief fire officer Purcell says fire officials made sure hospital officials reset the air handling system so it did not suck the smoke inside.
"We've still probably got 50 firefighters on scene and we'll be here for some time given the damage caused to the building," he said.
"It becomes a little bit difficult to put the fire out when the internal walls collapse."
Topics: industrial-fires, epping-3076
First posted


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An improved method for isolation of RNA from bone
Bone physiology is increasingly appreciated as an important contributor to metabolic disorders such as type 2 diabetes. However, progress in understanding the role of bone in determining metabolic health is hampered by the well-described difficulty of obtaining high quality RNA from bone for gene expression analysis using the currently available approaches.
We developed a simple approach to isolate bone RNA that combines pulverizing the bone and the phenol-guanidinium based RNA extraction in a single step while maintaining near-freezing temperatures. This single step method increases the yield of high quality RNA by eight-fold, with RNA integrity numbers ranging from 6.7 to 9.2.
Our streamlined approach substantially increases the yield of high-quality RNA from bone tissue while facilitating safe and efficient processing of multiple samples using readily available platforms. The RNA obtained from this method is suitable for use in gene expression analysis in real-time quantitative PCR, microarray, and next generation sequencing applications.
Obtaining intact, high quality RNA is an essential step in analyzing gene expression. This step is particularly challenging in bone, which contains low numbers of cells embedded within a highly mineralized tissue. As the endocrine functions of bone
Current methods for isolating RNA from bone use multiple steps in which the frozen bone is wrapped in foil, refrozen in liquid nitrogen and ground into a powder using a hammer
Figure 1
Overview of the One-Step Method for Isolation of RNA from Bone
Overview of the One-Step Method for Isolation of RNA from Bone.
Extracting RNA from bone in a single step
All animal studies using C57BL/6J male mice were performed with approval from the Pennington Biomedical Research Center Institutional Care and Use Committee using mice purchased from Jackson Laboratory (Bar Harbor, ME). Femur bones were harvested from five month old male mice that were fed a defined low fat (D12450B, Research Diets, Inc. New Brunswick, NJ) or high fat diet (D12451, Research Diets, Inc) beginning at four weeks of age. Any attached tissue was quickly removed from the bone using a scalpel before the bone was snap frozen in liquid nitrogen. The bone was stored at -80°C for up to two months before isolating the RNA. For RNA isolation, each bone sample was transferred from -80°C storage to liquid nitrogen until it was divided into two equal portions. To divide the bone, the femurs were cut using diagonal pliers (6 inch/solid joint, TopMost) that are available at hardware stores. One half of the bone was added to a microtube (1.5 ml Eppendorf Safe-Lock) that was prechilled by placing the microtube in a rack surrounded by liquid nitrogen. The remaining half of the bone was immediately returned to the liquid nitrogen and then stored at -80°C.
To facilitate processing multiple samples, we used the Bullet Blender (Next Advance) centrifuge technology that homogenizes tissue using bead disruption of the tissue. A four hour incubation of previously isolated liver RNA with RNase-free beads or untreated beads (Next Advance) demonstrated that RNA is not degraded when in contact with the untreated beads (Figure
The bone was added to a prechilled microtube containing the beads recommended by the manufacturer for RNA isolation (~50 μl stainless steel blend, 6 × 3.2 mm stainless steel, and 1 × 4.2 mm stainless steel) followed by the addition of 1 ml TriReagent (Molecular Research Center). The bone was homogenized in the Bullet Blender centrifuge for five minutes at a speed setting of "ten". The solubilized bone extract was separated from the beads and pulverized bone material by centrifugation at 8,600 ×
Figure 2
Extracting High Quality RNA from Bone in a Single Step
Extracting High Quality RNA from Bone in a Single Step. (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).
To determine if our approach is applicable to other methods of homogenizing the bone tissue, we repeated the procedure using a Polytron (Kinematic PT 3100, 7 mm tip) to homogenize the tissue (Figure
We compared this approach to currently used methods that employ separate steps to pulverize the bone and extract the RNA
RNA Quality and Yield
RNA yield was determined by the absorbance at 260 nm (Table
Table 1
RNA Yield and Quality Using a One-Step Approach to Extract RNA from Bone
Yield (μg)
26.0 -/+ 7.76
2.09 -/+ 0.015
2.17 -/+ 0.163
8.28 -/+ 0.87
3.17 -/+ 3.04
1.91 -/+ 0.155
1.09 -/+0.760
5.65 -/+ 2.15
RNA yield is reported based on the absorbance at 260 nm using a Nanodrop spectrophotometer and confirmed using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA quality is reported as the ratio of the absorbance at 260 nm and 280 nm along with the ratio of the absorbance at 260 nm and 230 nm as well as the RNA Integrity Number based on the entire electrophoretic RNA sample trace produced using the Agilent 2100 Bioanalyzer. Each value is reported at the mean -/+ standard deviation.
To demonstrate the suitability of the bone RNA isolated via a one-step approach for quantitative real-time RT-PCR, 1 microgram of RNA was reversed transcribed using Multiscribe Reverse Transcriptase (Applied Biosystems) with random primers at 37°C for 2 hour. Real-time PCR was performed with TaqMan chemistry (Applied Biosystems) using the 7900 Real-Time PCR system (Applied Biosystems) and universal cycling conditions (50°C for 2 minutes; 95°C for 10 minutes; 40 cycles of 95°C for 15 seconds and 60°C for 1 minute; followed by 95°C for 15 seconds, 60°C for 15 seconds and 95°C for 15 seconds). All results were normalized to a 18S rRNA expression. The gene expression of the bone specific transcription factor Runt-related transcription factor 2 (Cbfa1/Runx2; ABI Mm00501580_m1, amplicon length 129 bases)
Figure 3
The Isolated Bone RNA Supports Gene Expression Analysis using Real-time qRT-PCR
The Isolated Bone RNA Supports Gene Expression Analysis using Real-time qRT-PCR. One μg of the isolated RNA was reverse transcribed using Multiscribe Reverse Transcriptase (Applied Biosystems) with random primers at 37°C for 2 hours. Gene expression of the bone specific transcription factor Runt-related transcription factor 2 (Cbfa1/Runx2) and the adipocyte-specific transcription factor Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) was analyzed via real-time PCR performed with TaqMan chemistry using the 7900 Real-time PCR system and universal cycling conditions. The Runx2 and PPARγ TaqMan primer-probe pairs were obtained from Applied Biosystems. (A) The amplification plot and (B) standard curve generated for Runx2. (C) Comparison of the gene expression of PPARγ and Runx2 from bone RNA isolated from the one-step approach versus multi-step methods. The bone was obtained from an animal fed a high fat diet for 4 months.
In this study, we present a simple method for isolating bone RNA that relies on homogenizing the bone tissue using reagents and materials that are maintained at temperatures near freezing during a single step that combines fragmentation of the bone with extraction of the RNA. This practical and low cost method is readily adaptable to any homogenization equipment that is compatible with cooling the reagents and materials to near freezing. The results demonstrate that a single step approach consistently gives higher yields of intact RNA with RNA Integrity Numbers necessary to support demanding gene expression applications when compared to current methods.
This approach consolidates extraction of RNA from bone into a single step that allows routine isolation of high quality bone RNA from multiple samples without sample loss or possible cross contamination. This approach will facilitate studies designed to investigate changes in bone gene expression by ensuring a high yield of RNA suitable for demanding gene expression applications.
Authors' contributions
LEC participated in discussions to develop the method, isolated the RNA, carried out the agarose gel chromatography and real-time RT-PCR assays and participated in drafting the manuscript. LEC and ZEF constructed the figures for the manuscript. GK participated in discussions to develop the method, assisted LEC in the real-time RT-PCR, carried out the analysis of RNA quality using the Agilent Bioanalyzer and edited the manuscript. JMG participated in discussions regarding the method development and edited the manuscript. ZEF participated in discussions regarding the method and wrote the manuscript. All authors read and approved the final manuscript.
This study was supported by the American Diabetes Association (grant 1-10-BS-55, ZEF) and the National Institutes of Health (R56DK089020-02, ZEF). This project used Genomics Core facilities that are supported in part by COBRE (NIH P20-RR021945) and CNRU (NIH 1P30-DK072476) center grants from the National Institutes of Health. The 3.2 mm diameter stainless blend beads, 1.4 mm diameter stainless blend beads, and 4.8 mm diameter stainless beads were donated by Next Advance.
Bone as an endocrine organ
Playing with bone and fat
Analysis of gene expression in bone by quantitative RT-PCR
A reliable method for total RNA extraction from frozen human bone marrow samples taken at diagnosis of acute leukaemia
In vivo parathyroid hormone treatments and RNA isolation and analysis
Gene expression patterns in bone following mechanical loading
The RIN: an RNA integrity number for assigning integrity values to RNA measurements
Impact of RNA degradation on gene expression profiling
Minireview: Transcriptional Control of Osteoblast Differentiation
Obesity reduces bone density associated with activation of PPARgamma and suppression of Wnt/beta-catenin in rapidly growing male rats


text:
Buy-sell agreements be alive in many, if not most, closely command businesses having significant size and/or importance. And they be there concerning firm collective endeavour partners in umteen thousands of enterprises.
Buy-sell agreements are agreements by and between the shareholders (or assets partners of anything statutory depiction) of a in camera closely-held concern and, perhaps, the business organization itself. They found the mechanism for the acquisition of shopworn behind the change (or opposite adverse changes) of one of the owners. In the satchel of corporate integrated ventures, they as well embed the significance for break-ups or for destiny career for one firm project married person to buy out the some other partner. Buy-sell agreements (or put agreements in several cases) are more burning than supreme business owners, shareholders and boarding of directors recognize. I've frequently said that buy-sell agreements are cursive underneath the guess that the otherwise better half is active to die opening - and one of the partners is right! Seeing two antithetic agreements of late put the message at the top of my cognition and triggered a twosome of memories, as economically.
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The new day I reviewed a buy-sell understanding that was clearly magnificent on the day it was subscribed by a company's two central shareholders - more than than ten geezerhood ago. The agreement states that the parties will device the pro each twelvemonth. Since then, the group has more than tripled in volume and pro. However, the valuation in the buy-sell once it was signed object in issue nowadays because it was ne'er updated. This creates no consequential worries - unless thing harmful happens to one of the shareholders. In that case, one shareowner would reward from a bargain acquisition fee and the other's inherited would suffer a echt economical loss. With this point now in the open, those shareholders are on the job to news the writing as speedily as researchable.
Formula Pricing
Many firm owners privation to originate a steps to start the valuation if a buy-sell statement is triggered. And fairly a few agreements have them, in the main beside calamitous long-term grades. However, this is not uncommon because formulas confer an (apparently) cut-price alternative to hiring a company judge. Almost everybody can put a few numbers into a formula, whether it calls for manuscript plus at the prefatory fiscal closing or 4.5 modern world a 3-4-5 year (pick one) mediocre EBITDA - little debt, of flight path. (I've in fact seen the eviction of liability to determine equity utility omitted as chunk of the formula!) The questions is, will steps results be carnival for all sides in all circumstances? I won't be it here by uninteresting you near bigeminal examples, but no inflexible technique can realistically make certain the worth of a business concern over and done with clip with changing company, industry, and system provisions. That's why many a buy-sell agreements use an classification practice.
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Three Appraisers
As mentioned above, I reviewed two buy-sell agreements only just. The 2nd understanding involved the use of what I bid "one-two-three appraisers, rock!" The drafters of this like of understanding appear to assume that if it is favorable to bear one judge to merit a business, it is amended to bear two, or even cardinal. As an appraiser, I suppose I should like this appliance. After all, it increases the likelihood of our set man employed. While I don't cognise the genesis of this, umteen agreements are left-slanting wherever the rating machinery involves bigeminal assessment firms. Variations go like this:
- The purchasing gathering shall carry one independent appraiser, and the merchandising political party other. They will both afford appraisal opinions. If the belief are inside 10% or 15% or 20% (pick-a-percent), the charge for the buy-sell statement will be the norm of the two. If they are more than pick-a-percent apart, the price will be resolute by the middle of the ordinal appraiser's plus and that of the one nearest to him or her.
- The purchase gala shall retain one self-sufficient appraiser and the marketing gathering a ordinal. They do not provide appraisals. Rather, it is their job to reciprocally select a ordinal appraiser. Having been one of the artistic two appraisers in respective situations, I can william tell you that this is not as soft as you might think! This 3rd appraiser will furnish a rating of the concern (or curiosity). The ordinal appraiser's finishing point is the united upon selling expediency. If you are the third appraiser, that's an impressive responsibility, one that I've undertaken on various business.
- The purchasing get-together shall retain one autarkic judge and the marketing party a 2d. Both will furnish rating conclusions which, if ambient enough unneurotic (pick-a-percent), will be averaged. If the conclusions are more than than pick-a-percent apart, the imaginative two appraisers shall prize a 3rd appraiser. Again, this is not as effortless as one possibly will contemplate. The third valuator must past collect one of the two appraisals as the more correct valuation, and that will be the transaction terms. That's pretty dicey, too, and I've through it.
And near are in all likelihood other than variations on this focus.
The Bottom Line
You probably don't put in more instance at darkness rational something like your (or your clients') buy-sell agreement(s). Take my declaration for it, you shouldn't. You should be intelligent almost your buy-sell understanding now, in the airy of day, and on the job to get a observable agreement that building complex for you and your lad shareholders or partners. I never dry run law, because to do so requires a permit. So I don't have any permissible opinions. I prefer to believe of them as conglomerate opinions.
- If you are a conglomerate property owner or shareholder and your buy-sell agreement has not been updated in the closing yr (or if you don't fathom out it if it has), run, don't walk, to your corporate professional person to have a chat finished these issues. If you or your attorneys don't apprehend the rating nuances of your buy-sell agreement, don't waste time to bring out in a well-qualified enterprise valuator to read the agreement from a appraisal perspective and to relate you what he or she thinks it money - or if at hand is lawfully-begotten liberty for interpretation between appraisers. Find out what requests to be done, spawn the important decisions, and fix the script. It will never be easier than truthful now.
- If you are a trustworthy consultant to a conglomerate proprietor or epochal shareholder, I would propose production interaction for the shocking aim of discussing the buy-sell understanding and subjecting it to nominal reassessment and/or revision.
- If you are an enforcement or manager of a hulking company beside ninefold communal ventures involving considerable resources, you can transport remarkable plus point to your joint venture by requesting a review, from sub judice and appraisal viewpoints, of all alive agreements and/or put provision next to appraisal-type valuation mechanisms.
Remember this roughly buy-sell agreements - human will buy and human will put on the market. You merely don't know who that will be once you value the statement. Your statement of necessity to profession for you and your kith and kin whether you are the purchaser or retailer. And it requests to employment for your partner(s) and their families (or their shareholders) whether they are the buyers or peter sellers.


text:
In late January, I have found and reported a Server-Side Request Forgery (SSRF) vulnerability on toolbox.googleapps.com to Google's VRP, which could be used to discover and query internal Google DNS servers to extract all kinds of corporate information like used internal IP addresses
RCESEC-2016-012: Mattermost <= 3.5.1 Error Page Cross-Site Scripting / Content Injection
I'm quite busy with bug bounties lately, but sometimes I still discover stuff, which might also be interesting for the rest of you ;-). So here's quick writeup about a quite interesting vulnerability in the open source Slack-alternative Mattermost, which I have found in December
HamburgSides 2016: Magic Superpowers!
The year 2016 comes to an end quickly and so it was time for another Sides conference. This year's HamburgSides, formerly known as BSidesHH, was held in the Bucerius Law School in Hamburg next to the 33C3. I've been supporting
44CON London 2016: When Hackers Meet a Corgi!
Have you ever been to 44CON in London? In case you haven't, you need to go there in 2017! To be honest it was my first time attending, but it took just one 44CON for me to become excited and that not only because of
SLAE Course and Exam Review
As you may have noticed, I have posted a couple of articles about my SecurityTube Linux Assembly Expert exam during the last months. Now that I have successfully completed the course, I just want to share my thoughts about it for those of you
SLAE: Custom Crypter (Linux/x86)
Do you want to fool antivirus software? When you look through hacking forums for a solution to this, you will likely encounter the term "crypter". You will also find this tool in the arsenal of every advanced penetration tester and it
SLAE: Polymorphic Shellcodes (Linux/x86)
Question: How can signature-based Intrusion Detection systems be defeated? Answer: Using polymorphic shellcodes! This might sound really crazy and cyber, but it has nothing to do with inventing fancy new hacking techniques, it's rather about puzzling. By replacing assembly instructions with other assembly instructions
Ubiquiti Bug Bounty: UniFi v3.2.10 Generic CSRF Protection Bypass
Better late than never. This article will give you some insights about my discovered generic Cross-Site Request Forgery Protection Bypass in Ubiquiti's UniFi v3.2.10 and below, as published some time earlier this year on HackerOne. This vulnerability basically allows an attacker to compromise
BSidesHH: Ambiguity is Insecurity
Hamburg – just about one year ago, the first BSides was organized by Arron and Caroline. Now, one year later on 28th December, just after a relaxed Christmas time, they invited again – for the next #BSidesHH. It was powered by the great
CVE-2015-5956: Bypassing the TYPO3 Core XSS Filter
TYPO3 is the most widely used enterprise content management system with more than 500.000 installations. I have recently discovered a Non-Persistent Cross-Site Scripting vulnerability in its core and disclosed the details of the vulnerability publicly as CVE-2015-5956. This blog article should give you


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Advice for Puppy Buyers
Mini wire dachshunds are delightful, happy dogs and no prospective owner should be put off owning one when it is relatively easy to find a puppy that is not at risk. The more puppy buyers who are informed and ask the right questions, then the more dogs will be tested. As with any other puppy, do your research and:
- buy only known status puppies.
- do not avoid buying carrier pups – they will NOT develop the condition. Breeders have a responsibility to avoid narrowing the gene pool further by only breeding clear/clear, as this may result in introducing other inherited conditions.
- do not accept a breeder's word that 'they have never seen Lafora in their dogs. The only way to guarantee a dog will not develop Lafora is through breeding 'safe' combinations. Ask to see test certificates and use the WHDC public database to check the status of the puppy's parents.
- Read the excellent DachsFacts sheet from the Dachshund Breed Council: Lafora and Information for Potential Dachshund Owners
- Look out for our new Lafora Pioneer Breeders Scheme, coming soon!


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Receive our special offers & featured listing once a month
The Publications listed below can be purchased at the Balispirit Shop and KAFE, located on the first floor of the The
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The ACC is committed to providing Anthem community members with timely, accurate, comprehensive reports on the financial health of the community. This is done in many forms, including through the financial reports that are presented at the ACC Board's monthly meetings.
In addition, the ACC is required, per its bylaws, to prepare an annual report within 120 days after the close of the fiscal year that includes a balance sheet; an operating (income) statement; and a statement of cash flows for the fiscal year. Such a report is prepared on an audited, reviewed or compiled basis, as the Council Board determines, by an independent public accountant. This report takes the form of the annual audit which is posted here every year after Board acceptance.


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Tiwari has also been named as vice captain in the 14-member new-look team, to be led by T Ambati Rayudu, for third and final four-day 'Test' to be played at Jamshedpur from February 2-5.
The one-day matches would be played at Agartala (February 8 and 9), Guwahati (Feb 11 and 12) and the final will be at Siliguri (Feb 14).
"We have given stress on including players who would be eligible for the 2006 under-19 World Cup. So players like T Ambati Rayudu and Sikhar Dhawan have been kept out,"sources in the junior national selection committee said about the team for one-dayers.
The selectors have picked up a new-look side for the third 'Test' bringing in 10 players who were not included in the squad for the first two 'Tests'.
"This has been done as per the BCCI's policy of giving international exposure to the maximum number of players at the junior level," the sources said.
Team for the third Test: T A Rayudu (capt), Manoj Tiwari (vice captain), Cheteshwar Pujara, Ganesh Satish, Prashant Naik, Azar Bilakia, Pervez Aziz, R Kini, Sahbad Nadeem, Debabrata Paul, Y Mahesh, Abid Nabi, Piyush Chawla and Harsh Goutam. Coach: Venkatesh Prasad.
Team for the one-day matches: Manoj Tiwari (capt), Sushant Matathe, Ankit Rawat, Anirudh Srikant, Ravi Kant Shukla, Parag Madkaikar, A G Pradip, Rohit Sharma, Sahbad Nadeem, Piyush Chawla, Gourav Dhiman, Anand Rajan, Kuldip Sharma and Mayank Tehlan. Coach: Venkatesh Prasad.


text:
Mami Adachi Earns Third SEC Freshman of the Week Honors
LEXINGTON, Ky. - For a program-record-tying third time this season, University of Kentucky women's tennis player Mami Adachi has been named the Southeastern Conference Freshman of the Week.
Adachi is the second player in school history to earn three or more league accolades in the same season. She joins Megan Broderick, who was named the conference's Freshman of the Week three times in 2008. Adachi already became the first player in program history to earn back-to-back Freshman of the Week honors when she was recognized on Jan. 28 and Feb. 4.
A native of Tokyo, Japan, Adachi joined UK at the beginning of the semester and has made an immediate impact in Lexington. To earn her third SEC Freshman of the Week accolade, Adachi helped UK to a 4-3 upset victory over No. 15 Ole Miss in the Wildcats' SEC opener before a 5-2 victory over Mississippi State on Sunday.
On Feb. 27 vs. the 15th-ranked Rebels, Adachi posted a 6-4, 6-4 victory over No. 109 Mai Kamash. The win put UK within one point of clinching the upset victory, Kentucky's first over Ole Miss since 2007. On March 1, Adachi downed No. 63 Martina Frantova, 6-3, 7-6 to give the Wildcats a 3-0 lead over Mississippi State.
In singles action, Adachi has won 11 consecutive matches, all in straight sets, dating back to Jan. 13.
Paired with Aldila Sutjiadi at No. 1 doubles, Adachi earned an 8-3 win over Ole Miss and an 8-4 victory over MSU. Adachi and Sutjiadi where the first doubles pair to win their matches in both of UK's contests to open the SEC season.
No. 20 Kentucky returns to action vs. No. 13 Vanderbilt. Originally scheduled for Friday, the contest has been rescheduled due to weather for Tuesday, March 10 at Noon ET.
Visit UKathletics.com for the most up-to-date information on the Kentucky women's tennis team and all 22 varsity sports. For additional info, notes and behind-the-scenes updates, follow the team on Twitter at @UKWomensTennis.


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Wayang Museum
Wayang is a theatrical performance employing puppets or human dancer. The puppet could be made of leather which perform shadow puppets play, or of wooden. Wayang Museum (used to be called as Shadow Puppet Museum as well) is a museum which keeps collections of wayang from various territories in Indonesia and even from other countries. The idea of building Wayang Museum started when H. Ali Sadikin, the governor of DKI Jakarta, attended the second wayang museum week in 1974. With the support of the committee of the wayang museum week, wayang lovers, and the governor of DKI Jakarta, then the building at Jalan Pintu Besar Utara # 27, West Jakarta decided to be Wayang Museum. The museum was officially open on 13th August, 1975 by the governor of DKI Jakarta, H. Ali Sadikin.
The location of Wayang Museum is near to Cafe Batavia, Fine Art and Ceramic Museum and Fatahillah (History Museum) which are also interesting places to visit.
Jln. Pintu Besar Utara No. 27
Jakarta Barat 11110
Ph. 021 6929560
Opening Hours:
Tuesday - Thursday & Sunday: 9:00AM - 3:00PM
Friday: 9:00AM - 2:00PM
Saturday: 9:00AM - 1:00PM
Monday & Public Holiday: Closed
Entry Fees:
Adult: IDR 2.000
Student: IDR 1.000
Children: IDR 600
Travel Jakarta tour >>>
Jakarta Airport Transfer (JAT)
Jl. Jembatan Tiga Raya 5AH
Jakarta Utara, 41150