text:
I recently installed a Windows 2008 Foundation server. I set it up as the only DC, with DNS and DHCP.
Initially I received errors regarding licensing that indicated that the server would shut down within in a certain amount of days if it wasn't corrected. The error further indicated that the licensing problem was because it couldn't access a DC to figure out how many users there were. What is strange is that Windows 2008 Foundation is primarily designed for a small business that only has one DC.
We did successfully go through the process of properly activating the license. I also attempted to find information on how to setup Foundation as the only DC on a network but I couldn't find any definitive information.
My question is how can I make sure that I've licensed the server correctly and it won't shut down unexpectidly? Foundation only supports 15 users and I only have two on the server now. Is there any "clear" documentation on using Foundation as the only DC?
May 20, 2010 at 7:24 UTC
5 Replies
May 20, 2010 at 7:24 UTC
May 20, 2010 at 8:51 UTC
This is what I came accross the other day. In fact, the first two errors are the ones we saw initially. (The errors have gone away, but I'm not convenced that the problem has.) The problem with this article is it doesn't give any resolutions in a senerio where the Foundation server is the only DC. It also doesn't tell you the best way to setup a single server network using Foundation.
May 20, 2010 at 6:00 UTC
Niagara Technology Group (NTG) is an IT service provider.
What info do you want on how to run FS as a single DC? I'm unclear on what you want to know. Basically, you run everything on that one server because it is all that you have. Not too much to know in that scenario.
May 30, 2010 at 10:09 UTC
Was your question answered? If so, please mark best answer and helpful posts.
Aug 10, 2010 at 11:51 UTC
Yes, all is good.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Only a few minutes until the finale of Survivor, and, as it has been since before the first episode even aired, my money is on Eliza.
Why? Well, first, I've got to give props to a woman who is pre-law and interned at a public interest organization.
Second, she's been on the chopping block at nearly every tribal counsel, but continued to survive. (And isn't that what the game is all about?)
Now, of course, the people I like NEVER win (i.e. Rupert), but still, I've gotta say, Let's Go Eliza!
"Cops wanna knock me, D.A. wanna box me in. But somehow, I beat them charges like Rocky." - Jay-Z
Survivor Finale
by Blonde Justice on 12/12/2004
3 comments:
I'm sad that Eliza didn't even make it to the final 3. I didn't really like Brian, and I thought that was silly of him to get rid of Eliza. She seemed to really be on his side, whereas it was apparent that the other two were in an alliance.ReplyDelete
I'm hoping for a better time next season!
Yeah, Eliza didn't make it. But, like I said, the people I like never win.ReplyDelete
So please don't like me.ReplyDelete
Subscribe to: Post Comments (Atom)

subset: commoncrawl


text:
The Enhanced 4-Port FXO Voice/Fax Expansion Module supports the same density and features as the EM-HDA-4FXO, but provides enhanced features. It include longer loop length support of greater than 8k feet and improved performance against poor line conditions when used in ground start signaling mode.
Electronics, Computer & Photo > Computers & Accessories > Networking Products > Network & Communication > Routing/Switching Devices > Modules


text:
Chatswood - 2 bedrooms
Apartment, Chatswood
Confirmation within 24hr
From $190 per night {{quote.pricing.message}} Total {{quote.pricing.price | format:'currency':'short'}} for
Min 1 - 7 nights stay
Two bedroom apartment located one minute walk from Chatswood train station and Bus interchange.
A comfortable apartment with a large balcony.
Internet and Cable TV Foxtel included in the daily rate.
The minimum stay is 7 nights.
A Two bedroom on the 6th floor -Opposite Chatswood station & Bus interchange
Fully Air conditioned -One queen bed plus – 2 single beds , two bathrooms bathrooms with hair dryer, shower
Fully equipped granite kitchen with Smeg appliances & cutlery and cooking utensils
Dishwasher, oven, microwave, gas stove, large refrigerator - DVD player
Internal laundry with Washing machine & Dryer -Security card access to all floors
External Swimming Pool in the building - Balcony with table & chairs
Features covered by Stayz Rental Assurance
Air conditioning, Dishwasher, Parking, Washing machine, Wheelchair accessible
Rating snapshot
Average customer ratings
No reviews have been submitted for this property.
{{review.SubmissionTime | format:'date':'full'}}
{{comment.UserNickname}} responded to this review
{{comment.SubmissionTime | format:'date':'full'}}
Have a question?
Feel free to send a message to the owner/manager if you have any specific needs, or if you require additional information before you can commit to a booking request.
Select request booking to access the quote calculator and to check availability.
Enter your check in and check out dates to see availability and a total price.
|Sun to Thurs ($ p/n avg.)
|Fri & Sat night
|7 night stay
|Min night stay
|Extra adult ($ p/n)
|Extra child ($ p/n)
|{{tariff.weekdayMinimumRate | format:'currency':'short'}} -
|{{tariff.weekendRate | format:'currency':'short'}} -
|{{tariff.weeklyRate | format:'currency':'short'}} -
|{{tariff.extraAdultCost | format:'currency':'short'}} -
|{{tariff.extraChildCost | format:'currency':'short'}} -
The following dates have a special rate or exception applied. Please note any discounts applicable to these dates will still apply.
|{{exception.priceInCents / 100 | format:'currency':'short'}} p/n
|Security Deposit
|{{rates.bond | format:'currency':'short'}} p/s
|{{extra.valueInCents /100 | format:'currency':'short'}} p/n p/p p/np p/s
|Yes No
p/n = per night, p/s = per stay, p/p = per person, p/np = per night and person. Ask the owner to arrange optional extras
Note: Pricing is subject to change, and a minimum stay period and other conditions may apply. You should confirm pricing with the property owner. Bookings paid through the Stayz online booking system incur a 2% Service Fee.
Prices listed based on 4 adults. Property has 3 beds and can sleep a maximum 4 people.
- Available
- Unavailable


text:
Resort is located on famous Calangute Beach, on Baga Beach Road, and in close proximity to Vagator and Anjuna beaches. Resort is easily accessible from all parts of the Goa. Airport is about 48 km away, while nearest railway station is located at Thivim about 25 km from the hotel.
Die Verfügbarkeit Ihrer ausgewählten Reise wird geprüft.


text:
Futureshow Station, now Unipol Arena, is an indoor sporting arena located in Casalecchio di Reno, Province of Bologna, Italy. The seating capacity of the arena is for 11,000 people and it was opened in December 1993. It is currently home to the Virtus Bologna basketball team.
February 22, 2009Edit
Gaga performed during the half-time break of the Tim Cup Final with Michael Silas, Ian McKenzie, and Asiel Hardison.
- Set list:
- "Poker Face" (Elements of the Space Cowboy Remix)
- "Just Dance"


text:
I think the cutest thing about "LiberalLogic101″ is the complete lack of thought that goes into the memes they produce. Take the one above. Seriously, take it and keep it. The sheer ignorance involved is impressive. It's a source of wonder the creator can even figure out how to turn on a computer much less copy and paste a right-wing talking point.
Standard Right Wing Propaganda – Guns Aren't Dangerous, Black Presidents Are!
Current Status: Blessed (1)
Seeded on Thu Jan 24, 2013 2:39 PM
keyboard shortcuts: V vote up article J next comment K previous comment


text: ok, right now as a new forum, i don't want set any permissions to category/forum or node. All i want to do is create user groups and give them permission. then, shall i set all the permissions for all nodes to all user groups to INHERIT is this right? Btw which takes more priority Forum Permission or User Group Permission


text:
Product Details
Style: WR609
Function: The Brooks Epiphany run short is designed to elevate your running experienceand elicit compliments along the way. Mesh splicing enhances breathability, an inner liner moves moisture away from the skin, and stretch fabric moves with you for full freedom of movement. Youll want one in every color (we do). Fabric: Shell: Revelation Stretch Woven (86% polyester/14% Lycra spandex); Liner: 100% moisture transfer polyester is ultra-lightweight, fast-drying and cool to the touch, with a fine, buttery soft texture.
- Fit: Semi-fitted
- 3.5 inseam
- Stretch fabric ensures freedom of movement
- Mesh splicing enhances breathability
- Moisture transfer liner keeps you dry and comfortable
- Draw string helps you find your perfect fit
Questions and Answers
ASK A QUESTION Got questions about this product? Ask them here. Got questions about anything else? Please see our FAQ.
More Like This
Our Price $24.00
Available Sizes:L, M, S
Our Price $14.99
Available Sizes:L
Our Price $14.99
Available Sizes:L
Our Price $29.99
Available Sizes:L
Our Price $29.99
Available Sizes:L


text:
Article recommendation
Wanted to direct everyone to a new online article about The Colorado Fly Fishing & Riparian Education Center in Pinecliffe, Colorado. Very cool thing these guys are trying to get up and running.
Center Seeks to Educate Colorado Kids About River Ecology - Yahoo! Voices - voices.yahoo.com
|All times are GMT -5. The time now is 05:57 AM.
Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Search Engine Optimization by vBSEO 3.6.0
2005-2017 The North American Fly Fishing Forum. All rights reserved.


text:
I am not sure how to thank everyone a Crocodile Bay for a " Fantastic Trip . " Hopefully you can forward this message on to the appropriate individuals. Bo, Todd, Anthony, German and the gang were great! Everything went of like clock work. The fishing was everything that one could ask for and more. My daughter caught a 50 lb Rooster, and I came in second not far behind with a 45-pound Rooster, and we split the duty on a 40-pound Rooster. I did manage to bet her out with a 25 lb red snapper and my 57 " Barracuda. All of which will be seen in future article.
Sandy, Kree and I want to thank everyone for one of our most enjoyable vacations to date. I personally am looking forward to returning again and hopefully not in thirteen years.
- Official Description (provided by the hotel):
- All-inclusive packages for World-class Billfishing offshore or Roosterfish, Snapper, & Dorado inshore. Kids love our 24 exclusive Ecotours - Corcovado Rainforest, Zipline, Mangrove Kayaking, & Moms love our luxurious state-of-the-art Spa with a full complement of 30+ Spa massages, facials and full body treatments. Set on 44 lush tropical acres in the bio-diverse Osa Peninsula. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Orbitz, Travelocity and Hotels.com so you can book your Crocodile Bay Resort reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Crocodile Bay Hotel Puerto Jimenez
- Crocodile Bay Resort Costa Rica/Puerto Jimenez


text:
Greater Injury Kit Recipe is a herbalism recipe in Dragon Age: Origins.
Acquisition Edit
Dragon Age: Origins Edit
- Sold by Alarith at the Elven Alienage in Denerim for 00000792007 92 0.
Dragon Age: Origins - Awakening Edit
- Sold by Yuriah in the Vigil's Keep - Throne Room for 00000864008 64 0 after his stock have been upgraded three times.
- Sold by the Keep Medic for 00000180001 80 0 during The Siege of Vigil's Keep quest.
Crafting Edit
|Required skill
Greater Injury Kit Recipe


text:
Submitted by kallen • October 22, 2013 customwritingtips.com
The article tries to explain the concept behind the advancement of technology in the education setting. The advance of technology helps in transforming the shape of education in learning institutions in the future. In the article, the author has developed ways in which technology applies in shaping the education system.
- Category: Education
- Tags: 24/7 writing company


text:
It only takes a minute to get started. Fill out our uncomplicated sign up form and login to the site.
This step is elective but will greatly raise your chances of your profile being viewed by interested members. Write a few sentences about yourself, your interests and upload any nice photographs of yourself.
Utilise our website's sophisticated search to find members who meet your criteria. Or just scan through listings and find daters that take your fancy.
Found someone who appeals to you? Drop them a line or just a wink and see what develops from there. With a little luck you'll be on your way to true love.
You won't regret it


text:
Here's a hot deal. If you have a Cheesecake Factory restaurant nearby, you can snag a slice of cheesecake for 50% off on 7/29/16 and 7/30/16. There is a limit of one slice per guest and it is a dine-in only promotion.
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


text:
Does anybody need to be reminded how useful such file cabinets can be? This collection has proved to be a good thing for numerous customers and what do you think about all this? Even those people who have high expectations, did not have too many problems with deciding so neither should you.
Oxford 3-Drawer File Cabinet - File Storage - File Cabinets - Office Furniture |
It is a lateral file cabinet for storing your files and important documents. Everyone need to have this in office space for protect the files. It has got a cherry finish and elegant style.
Four Drawer Vintage Brushed Steel File Cabinet
Turn a trunk into a mini office with this how-to.
Green Antiqued Wood File Cabinet
Storing documents required comfortable and practical cabinets. It must give them very easy way to access and allow them to organize. Such is the cabinet. This combination of delicate elegance and usability.
24.5" tall utility stand. Sleek modern design, with two big drawers and a very handy open shelf above them. Perfect to put near your favorite chair or recliner so you can have every bill, paper, pen etc. at hand.
Sauder Harbor View 1 Drawer Lateral Wood File Cabinet in Antique White
This spacious drawer box cabinet will allow you to store files, documents and lots of small items securely while enhancing interior features with its neat and sleek construction. It has a locking mechanism.
metal filing cabinet dresser
File Cabinet~I found one at a garage sale 15.00, now want to paint with chalk paint and cover front with contact paper use for clothes
Lateral file cabinet. Two big drawers with interlocking drawer system. The cabinet is made of steel with smooth black finish. Sturdy and good quality. Perfect for storing your documents at home or at the office.
Very elegant, stylish and classic wooden cabinet with two drawers. It has delicate feet and very delicate decoration, which refers precisely to the classics. It is made with light, reddish wood. Perfect for the living room.
love. antique filing cabinet ~
This small but very functional mobile cabinet is the perfect solution for your office and home. Two practical drawers including one closed with the key in a safe location will store important documents and other items.
Decorating your office? You may want to check this 2-Drawer Vertical File Cabinet. Made of resistant steel, has high side-drawers and glide suspension. It can make your office to look more professional.
It is very practical and extremely useful cabinet documents. It consists of three drawers of different sizes, which facilitates the establishment of order. Drawers are slightly transparent. The whole is mainly practical, but also looks good.
This well-thought out cabinet for documents, is the perfect solution for your home or office. You can describe every drawer and organize documents discretion. Made from high quality steel.
Black, elegant and very practical cabinet for documents. It consists of three drawers where you can store a variety of files. The medium drawer is locked, the others are open. Ideal for offices, archives and agencies.
This file holder can be used for commercial purposes because it has been created of high-quality materials. Its classic black color makes it attractive for office areas. There's a locker to ensure that your files are safe.
Functional piece of furniture, suitable for dens, offices, studies and libraries. This 3-Drawer Steel File Cabinet in Black Finish features three letter-size drawers, and a convenient lock to secure the two top drawers.
This simple and very functional cabinet with two drawers is a great place for various documents and notes. Will check at home, in the office, campus - wherever you need it. Handy handles and the possibility of closing ensures the safety of your files.
This Functional 4-Drawer Vertical File Cabinet in Putty Finish features four smoothly operating drawers with cam locks and ball-bearing slide suspension. Each drawer includes a convenient pull handle, and small frame for placing tags.
This white file cabinet has two deep drawers and accommodates letter-size files. Its construction has been made of steel and is finished in pure white. It will look good in a doctor's office. You can secure both drawers with a locking mechanism.
This practical and attractive 18-Inch Mobile File Cabinet in Black Finish includes 1 open shelf for extra storage, 1 file drawer for letter-size hanging files, and 1 accessory drawer for office supplies. The whole construction is durable and fully-functional.
Useful choice for offices and studies, this Mobile Standard File with Lock in Black Finish is going to help you organize all your messy files. The file features a sliding cover, a key lock to secure files, a hinged top and 1 lower shelf.
Modern storage cabinet that will let you organize your documents and letters conveniently. Black finish accentuates contemporary looks. Silvery metal hardware also adds to a style. The cabinet is equipped with a sturdy lock.
Functional rectangular modern file cabinet. Sturdy wooden construction is made of wood finished in black. It has 2 gliding drawers with 2 metal handles each. The upper drawer is lockable with a key. Compact, suitable for smaller interiors.
A vertical file with extra lock to keep your belongings and documents utmost protected - it offers two drawers that open fully to provide easy access to the entire contents and the black finish makes for a neutral and elegant addition to any setting.
Storex Wheeled Two-Drawer Filing Cabinet, 18 Inches, Light Grey (61301B01C)
Ultra-practical seating: ottoman on lockable rolling casters, hiding a pull-out drawer inside! It is an alternative for ordinary storage ottomans. It is very mobile and utilitarian. It has hole-pattern black sides and cushioned top.
This practical and very effective three drawers pedestal is the perfect place to store documents and other trinkets. Robust construction will check on many occasions. It can serve as a side table or a table to the printer.
File cabinet. It is a very small, tiny and handy file cabinet. It has mounted wheels, so that it can be freely moved around the room. It consists of two small drawers and one more. It is very practical and useful.
Convenient and extremely practical file cart. This open cart, in which, the files are somehow suspended. It is a cart on wheels, so that, it can freely move. It is very useful. Of course, ideally suited to any office.
High cabinet for storing files. It was made in a bright color. It consists of four drawers, which are equipped with comfortable handles. It is primarily practical and useful, but no one will refuse her the elegance and style.
Cabinet for storing documents and other personal items. It is made of wood and mounted on wheels for easy movement. It has 2 drawers arranged vertically. Suitable for residential and commercial premises.
File cabinet made of charcoal steel, very durable. Three lockable drawers, also for hang folders. Beautiful matte gray finish, smooth drawer mechanisms, handy casters, and perfectly sized. Looks and functions great in an office.
Mahogany drawer cabinet. This elegant and practical furniture works perfectly in any home or work environment. Two spacious drawers are ideal for storing documents or other precious belongings. Secure the interior with the lock.
The wide, bright cabinet for the storage of documents and records. It was made in a bright color. It consists of four drawers, which allows for systematic organization of stored documents. Suitable for office, on the desk and the agency.
Slap Ceiling Tins on to old metal filing Cabinet? And use for end table and office storage?
Beautiful Paisley Peacock Modern Steelcraft Filing Cabinet
Filing cabinet remake
Use beautiful wallpaper to cover the front of an old filing cabinet. Wonderful!
House Dressing Style - makeover file cabinet
Peacock Filing Cabinet from House for Five
And have we got a file cabinet for you! House Revivals: Super-Amazing Ways to Up-Cycle Filing Cabinets!
Decorated File Cabinet ~
Cool file cabinet DIY.
FIle Cabinet Makeover-1-2
Mini office chest for #office #organization Great for a home office, because it can be used to sit on, and doesn't make your home look too "office-y"


text:
How to Dye Mohair Wigs?
Dyeing mohair wigs is a relatively easy step in the doll-making process. Before dyeing, you must ensure that the mohair wig is clean and does not have any tangles. It should also have plenty of roo...
Read More »
How to Dye a Mohair Wig?
The first doll to sport a mohair wig of which we have written history comes from Germany around 1880, and was introduced by the company Alt, Beck and Gottschalk. Mohair is still the choice of quali...
Read More »
How to Make Worn Out Wigs Into New Wigs?
Wigs can give you a completely different look for a special occasion, or be worn every day. Unfortunately, wigs, unlike human hair, all eventually start looking old. Buying new wigs every time your...
Read More »
How to Clean Mohair?
Mohair is the lofty, downy fiber from a goat. It is similar to wool in many ways, but it is warmer and less bulky. Mohair can be knitted or woven and is most often used to make sweaters and home ac...
Read More »
How to Apply a Reborn Mohair Wig?
Add new hair to your reborn doll with ease, by using a premade mohair wig. Some doll makers root single strands of hair into the doll's scalp with felting needles, to create wispy hair. This proces...
Read More »


text:
22-3281 - Basic Electronic Tool Kit
Kit contains: Full range digital multimeter (similar to #72-6343), plastic parts box, 5" diagonal cutters, 5" long nose pliers, 6" slanted cutters, 6" long nose pliers, 6" stainless steel scissors, 7-in-1 wire cutters/crimpers, three Phillips screwdrivers (#0 x 75...
200-040 - DeoxIT D5 Spray Contact Cleaner, 5% solution, 5 oz.
Fast acting deoxidizing solution cleans, improves conductivity, lubricates & protects.
DeoxIT contact cleaner dissolves oxides and sulfides that form on metal surfaces. This restores the contact's integrity and leaves a thin microscopic layer that protects t...
21-10754 - 8" Cable Ties - Black - 1000 per Pack
These cable ties are manufactured from Nylon 66 for higher heat resistance and are approved to UL-94V-2. Resistant to gas, oil, grease and alkalies.
298-0010 - AAA Heavy-Duty Alkaline Batteries - 40pk
40-pack Ultra Alkaline AAA Batteries
Pro-Elec Ultras are heavy-duty alkaline batteries suitable for all general purpose applications. High quality, leak-resistant, non-mercury and cadmium-free materials make these batteries a perfect choice for long-lasting, h...
MCM is a great company. Very fast service and shipping of Raspberry Pi. Will tell all my friends to buy from MCM


text:
Actors: Manuel Ferrara - Alexa Pierce
Categories: Hardcore - Brunette - Big Ass - Big Tits - MILF - POV
Source: manuelferrara
Description: Busty MILF Alexa Pierce is fucked hard by Manuel Ferrara!
Published on 2015-06-05
The content of this page is reserved for mature adults.
To view the page click on "Enter" or click "Exit" to return to the homepage.
Enter - Exit


text:
Contact Tiret & Company CPAs Today
Could your business benefit from expert help in tax, accounting, payroll or business management? Do you need assistance with your personal taxes or financial plan? Let Tiret & Company help you!
We serve businesses and individuals in the community of San Bruno, California.
1250 Bayhill Drive Suite 100
San Bruno, CA 94066
Phone: 650-225-0400
Fax: 650-225-0129
Payroll Fax: 650-225-0490
Email: firstname.lastname@example.com
If you would like more information, please complete the form below and we will contact you as soon as possible.


text:
2012 Performances
Sat Feb 25
Ecstatic Music Festival: Glasser & Roomful of Teeth, featuring the music of Merrill Garbus & Sarah Kirkland Snider
Merkin Concert Hall at Kaufman Center
A Glasser setlist is a compilation of all the songs Glasser has played live in concert. Many Glasser concerts have the same setlist, and sometimes Glasser setlists will vary from night to night. We've gathered together 2017 Glasser tour setlists as well as past Glasser setlists to give you a better idea of what you can expect at your local Glasser show. If you have a Glasser song list you would like to upload, please check back as that functionality will be added soon, as well as setlists for Glasser.


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Embedded content is displaying html infoAsked by eeeo_ed on March 17, 2015 at 03:03 PM
JotForm Support
Do you mean to say that the visual report is displaying html info? As I check the report link provided, I see that the report is displayed without any issue. Could you confirm if the issue occurs in any particular browser?
Please try clearing the browser cache and see if that helps. Please get back to us with little more information on the issue, if it still exists.
Thank you!
I just fixed it. What I think happened is that the chart was slightly overlapping the header. When I deleted the header, it solved the problem.
That being said, there are big spacing issues: when I set up the report, the chart is right up against the top of the page, yet when it's embedded, there is the huge gap you see in your screen shot.
JotForm Support
That's great to see that you were able to resolve the issue. We appreciate your efforts. With respect to the spacing issue on the top of the page, you may try moving the fields on the visual report designer to fit your needs.
Hope this information helps. Please let us know if need any further assistance. We will be happy to help.


text:
posted by CL .
When I measure pressure in relation to depth, I found that as the depth increases, so does the pressure. I experimented this in a LARGE graduated cylinder. What would be the difference if I used a SMALL graduated cylinder versus a large one? What is the evidence for this? And what is happening on a molecular level to the molecules in the smaller graduated cylinder?


text: CEI Today: Durbin price controls, union special privilege, and a public pension crisis August 29, 2013 Wednesday, August 28, 2013 In the News Today DODD-FRANK DURBIN AMENDMENT - JOHN BERLAUCredit Union Times: Flawed Durbin Ruling Boosts Fed's Odds: Guest Opinion Since U.S. District Judge Richard Leon issued his shocking decision on July 31 that called for even more draconian price controls under Dodd-Frank's Durbin Amendment, some legal commentators have given him the benefit of the doubt. Leon's decision oozes with unprofessional snark, misconstrues economic terms in the statute's language, and limits its research of "legislative intent" to the "intent" of one member of Congress who voted for the legislation: Sen. Richard Durbin (D-Ill.). Leon's ruling also forces the Fed to construe the law in a way that almost certainly will foment a constitutional challenge. Because of these many flaws, the Fed has a good shot at getting the ruling overturned in its pending appeal. > Read more > Interview John Berlau UNION SPECIAL PRIVELEGE - TREY KOVACS Workplacechoice.org: Union Special Privilege Under Scrutiny In response to a Miami Herald report, a Miami-Dade County Commissioner is sponsoring legislation to eliminate or reduce the practice of allowing government employees to work full-time for their union, also known as union release time. Union release time is an inappropriate use of taxpayer funds. > Read more > Interview Trey Kovacs PUBLIC PENSION CRISIS - IVAN OSORIOOpenmarket.org: Playing Politics with Public Pensions Many public pension plans around the nation are severely underfunded. The 2008 financial crisis, which wiped out many pension investments, has focused the public's and policy makers' attention on the issue since then. But the financial crash and ensuing Great Recession don't explain all of the problem public pensions face today. One major problem is self-inflicted: investment decisions that advance political agendas rather than shareholder value. > Read more> See also: Lobbyists at the State Public Trough > Interview Ivan Osorio More in the news... Historians Should Learn the Economic Way of Thinking Gore Hijinks: Everyone's Favorite Crazy Uncle Al Is On the Loose Again President Obama: Cut Law School from Three Years to Two Big Labor versus the Obama Administration New Mexico Court: Go Into Business, Lose Your First Amendment Rights CARBON TAX INEVITABLE? CEI is a non-profit, non-partisan public policy group dedicated to the principles of free enterprise and limited government. For more information about CEI, please visit our website, cei.org, and blogs, Globalwarming.org and OpenMarket.org. Follow CEI on Twitter! Twitter.com/ceidotorg. Before Net Neutrality Eats the World On Sept. 9, the US Court of Appeals for the D.C. Circuit will hear oral arguments in Verizon's challenge of the FCC order on net neutrality.Unelected FCC commissioners contemplate this action without authority from Congress, and even with such authority, it should have and would have been challenged. CEI President Lawson Bader The EPA doesn't have to tell anyone anything, or does it? INTERVIEW A CEI EXPERT Media Contacts:202-331-2277Christine HallNicole CiandellaBrian McNicoll CEI Experts by Issue Advancing Capitalism Constitutional and Legal IssuesEnergy and Environment Finance and Entrepreneurship Health and Safety Labor Nanny State Regulatory Reform Tech and TelecomTrade and InternationalTransportation


text:
Computer-based fluorescence quantification: a novel approach to study nucleolar biology
Nucleoli are composed of possibly several thousand different proteins and represent the most conspicuous compartments in the nucleus; they play a crucial role in the proper execution of many cellular processes. As such, nucleoli carry out ribosome biogenesis and sequester or associate with key molecules that regulate cell cycle progression, tumorigenesis, apoptosis and the stress response. Nucleoli are dynamic compartments that are characterized by a constant flux of macromolecules. Given the complex and dynamic composition of the nucleolar proteome, it is challenging to link modifications in nucleolar composition to downstream effects.
In this contribution, we present quantitative immunofluorescence methods that rely on computer-based image analysis. We demonstrate the effectiveness of these techniques by monitoring the dynamic association of proteins and RNA with nucleoli under different physiological conditions. Thus, the protocols described by us were employed to study stress-dependent changes in the nucleolar concentration of endogenous and GFP-tagged proteins. Furthermore, our methods were applied to measure
Our protocols set the stage to analyze in a quantitative fashion the kinetics of shuttling nucleolar proteins, both at the single cell level as well as for a large number of cells. Moreover, the procedures described here are compatible with high throughput image acquisition and analysis using HTS automated platforms, thereby providing the basis to quantify nucleolar components and activities for numerous samples and experimental conditions. Together with the growing amount of information obtained for the nucleolar proteome, improvements in quantitative microscopy as they are described here can be expected to produce new insights into the complex biological functions that are orchestrated by the nucleolus.
The proper organization of cellular organelles and compartments is of fundamental importance to eukaryotic life. Within the nucleus, nucleoli are key compartments that contribute to a growing number of cellular processes
Defining the composition and activities of the nucleolus under various growth conditions is a prerequisite to understand its role in different aspects of cell physiology. As dynamic compartments, nucleoli are characterized by a constant flux of macromolecules, in part due to their shuttling between nucleoli and the surrounding nucleoplasm. Advanced fluorescence microscopy and photobleaching techniques revealed the highly dynamic nature of the nucleolus which is characterized by the transient association of many nucleolar proteins with this compartment
Recent advances in proteomics indicate that up to several thousand different proteins could be associated with nucleoli
Results and Discussion
Quantification of nucleolar fluorescence is complicated by the dynamic nature of nucleoli
Measurements of organellar or compartment-specific concentrations of molecules frequently depend on a marker that identifies the subcellular structure of interest. It further requires that the marker detects a specific organelle or compartment under a variety of experimental conditions. However, in the case of nucleoli this approach is challenging, since several of the nucleolar "marker" proteins relocate in response to environmental or disease-induced changes. This is demonstrated in Figure
Figure 1
The dynamic organization of nucleoli requires new tools for quantification of nucleolar fluorescence
The dynamic organization of nucleoli requires new tools for quantification of nucleolar fluorescence. The nucleolar accumulation of B23 and fibrillarin, frequently used as marker proteins for the nucleolus, is sensitive to changes in growth conditions. HeLa cells were grown under normal conditions or exposed to a 1-hour heat shock at 45.5°C. Cells were fixed and processed for indirect immunofluorescence. Size bar is 20 μm. Note that a portion of either protein relocates from the nucleolus upon heat stress.
Figure 2
Quantification of nucleolar fluorescence is a multi-step process
Quantification of nucleolar fluorescence is a multi-step process. The quantification process requires three major steps as summarized in the figure. For each of these steps images are processed with different morphology filters that are indicated in the figure. See text for details.
To accommodate a wide diversity of experimental settings, we designed alternative methods for the analyses of images. These methods differ in the functions that are selected for each step of the quantification process. For instance, several strategies are used to identify nucleoli, and distinct functions are employed to reduce noise and false positives (Figure
The key step in the quantification process is the identification of nucleoli. Two groups of procedures demarcate the nucleolar compartment (Figure
Measurement of nucleolar fluorescence based on nucleoplasmic markers with low abundance in nucleoli
In principle, these procedures rely on a nuclear marker that is absent from nucleoli or more abundant in the surrounding nucleoplasm. For example, labeling nuclear DNA with a fluorescent dye, such as 4', 6-Diamidino-2-phenylindole (DAPI), is a simple procedure to generate such a marker. Alternatively, a nuclear protein like RNA polymerase II (Pol II) can serve as a marker, because there is little Pol II in nucleoli. For both markers, the nucleoli are defined as regions within the nucleus where fluorescence intensity declines. Here, we provide three different methods that depend on the distribution of a DNA marker and/or fluorescently labeled Pol II to identify nucleoli.
This method (Figure
Figure 3
Quantification of nucleolar fluorescence in HeLa cells synthesizing a GFP-tagged reporter protein that localizes to nucleoli and the nucleoplasm
Quantification of nucleolar fluorescence in HeLa cells synthesizing a GFP-tagged reporter protein that localizes to nucleoli and the nucleoplasm. The DAPI image is employed to locate nucleoli. Individual operations necessary to identify nucleoli and quantify pixel intensities in the nucleolar compartment are shown. (a) Confocal images for DAPI and GFP. (b) Identification of nucleoli based on DAPI-staining of the DNA. Dark holes in the DAPI image are detected with the
In Figure
Additional file 1
Multiple processing steps are applied to identify the nucleolar compartment. Examples of the settings for different operations are shown. (a) If fluorescence signals in nucleoli are below the pixel intensities in the nucleoplasm, the
Click here for file
Once the nucleolar compartments are identified, the software creates segments that represent nucleoli. The generation of segments is based on the size constraints and pixel intensity above background as they are provided by the researcher; this step is carried out for all of the methods illustrated here. The nucleolar segments are overlaid with the DAPI image (Figure
Applying the same principle as described for Method 1, the distribution of Pol II, or any other marker that is of low abundance in nucleoli, can also be used to identify nucleoli (Additional file
Additional file 2
Identification of nucleoli with Methods 1-4. (a) Original confocal images show the distribution of the DNA marker DAPI (blue) and Pol II (red) as well as a GFP-tagged protein (green, probe image, Stat. correction). (b) Method 1 identifies nucleoli based on the DAPI image. (c) Method 2 employs the Pol II staining to delimit nucleoli. (d) Method 3 demarcates nucleoli by using the DAPI and Pol II images (
Click here for file
Under some experimental conditions, reliance on a single nuclear marker may not be sufficient to identify nucleoli with high confidence. Furthermore, visual inspection to exclude false positives is not always feasible, in particular when large numbers of images are generated. To address this problem, we designed an alternative method that combines information from the DAPI as well as Pol II images to identify nucleoli (Figure
Figure 4
Identification of nucleoli based on the distribution of both DAPI and Pol II
Identification of nucleoli based on the distribution of both DAPI and Pol II. (a) Original confocal images of HeLa cells that transiently synthesize a GFP-tagged protein. Cells are co-stained with DAPI (blue) and antibodies against Pol II (red). (b) The
As shown in Additional file
Measurement of nucleolar fluorescence based on the probe image
To accommodate different experimental settings, we developed alternative procedures (Additional file
When the fluorescence in nucleoli is dimmer than in the adjacent nucleoplasm, nucleoli can be delimited with the probe image (Additional file
If the fluorescence in nucleoli is brighter than in the adjacent nucleoplasm, nucleoli can be identified with the
The concentration of proteins B23 and fibrillarin in the nucleolus is regulated by stress
We applied the approach described in Figure
Figure 5
Quantitative analysis of B23 and fibrillarin nucleolar localization
Quantitative analysis of B23 and fibrillarin nucleolar localization. The abundance of proteins B23 and fibrillarin in nucleoli is regulated by stress. HeLa cells were grown under normal conditions or heat-shocked for 1 hour at 45.5°C as shown in Figure 1. Nucleolar and nuclear fluorescence was quantified and pixel values/area were calculated. For B23, 21 control cells (38 nucleoli) and 22 heat-shocked cells (36 nucleoli) were measured; for fibrillarin 20 control (28 nucleoli) and 20 heat-treated cells (31 nucleoli) were analyzed. Results obtained for control conditions were defined as 1; significant differences were identified with two-tailed Student's
In Figure
Since many of the HeLa cell nuclei contain more than one nucleolus, we repeated the analysis after combining the measurements for individual nucleoli within the
For many proteins the balanced distribution between two different cellular compartments is critical for their biological function
Taken together, the results presented in Figure
Nucleoli can be identified with the Pol II image in heat-shocked cells
For some cell types stress may alter the DAPI staining and may therefore not be useful as the sole reference to demarcate the nucleolus. In Additional file
Additional file 3
Nucleoli can be detected in heat-stressed cells. HeLa cells were incubated for 1 h at 45.5°C, fixed and stained with antibodies against Pol II and RPA194, a subunit of RNA polymerase I. RPA194 was chosen as a marker for nucleoli, because it is less affected by heat than B23 and fibrillarin (unpublished data). Original images are shown in the top panels. Nucleoli are identified based on the Pol II staining (panels in the middle). This requires the
Click here for file
Comparison of different nucleoli located in the same nucleus
Many cell types have more than one nucleolus per nucleus
Collectively, the analyses for B23 and fibrillarin suggest complex changes in their intranuclear location in response to stress. The studies described in Figure
Measurement of nucleolar fluorescence in different cell lines
Given the diversity of cellular models used in different studies, it is crucial for the methods described here to be applicable to different cell lines. This is of particular importance as nuclear morphology and the pattern obtained upon DAPI staining can differ drastically (see for instance NIH3T3 cells in Figure
Figure 6
Quantification of nucleolar fluorescence intensities in different cell lines
Quantification of nucleolar fluorescence intensities in different cell lines. Nucleoli were identified in (a) MCF7 and (b) NIH3T3 cells using the methods shown in Figure 3 for HeLa cells. Note that the unique DAPI staining pattern that is characteristic for NIH3T3 nuclei does not interfere with the quantification protocol.
Analysis of the nucleolar accumulation of GFP-tagged hsc70
The detection of proteins by indirect immunofluorescence can be complicated by the accessibility of antigens under different conditions. Furthermore, many studies involve the analysis of reporter proteins that are fused to GFP or other fluorescent moieties. It was therefore important to demonstrate that the protocols described here can be applied to GFP-tagged proteins. In addition to the results shown in Figure
Figure 7
GFP-tagged hsc70 accumulates in nucleoli during the recovery from heat stress
GFP-tagged hsc70 accumulates in nucleoli during the recovery from heat stress. Transiently transfected HeLa cells synthesizing GFP-hsc70 were grown under normal conditions or exposed to a 1-hour heat shock at 45.5°C and then incubated at 37°C for 3 hours. Nucleolar accumulation of GFP-hsc70 was measured with method 1 in controls (n = 51) and cells recovering from heat stress (n = 71). The results for nucleolar fluorescence are shown as means + STDEV; data were normalized to the control, and significant differences were determined with Student's
Taken together, the methods described here are applicable to different procedures that are used to visualize proteins or RNA (see below). This includes the labeling with antibodies, use of fluorescent reporter proteins or other techniques that generate fluorescent reporter molecules.
Quantification of RNA synthesis in nucleoli
One of the hallmarks of nucleoli is the synthesis and processing of 45S ribosomal RNA and the subsequent assembly of ribosomal subunits. Transcription can be detected in growing cells with click chemistry, and nucleoli are prominently labeled with this technique
Figure 8
Measurement of RNA synthesis in nucleoli
Measurement of RNA synthesis in nucleoli. HeLa cells were incubated with 1 mM 5-ethynyluridine for 6 hours at 37°C, fixed and treated with azide-modified Alexa Fluor488. (a) Confocal images were acquired, and the probe image was corrected for background fluorescence (EU, Stat. correction). The
We further examined whether the staining pattern obtained for EU-labeling properly identified the nucleolar compartment. To this end,
To evaluate whether EU staining can provide a marker for the nucleolar compartment upon stress, we exposed cells to a 1-hour heat shock either before or after the incubation with EU. Under these conditions, EU labeling remained prominent in the nucleolus (Additional file
Additional file 4
The effect of heat stress on EU staining. For all experiments, HeLa cells were kept with EU for 6 hours, and EU was labeled with Alexa Fluor488 as described in the Methods section. In the top panels, cells were grown with EU at 37°C for 6 hours and fixed. Panels in the middle show cells that were first heat-shocked for 1 h at 45.5°C, then treated for 6 hours with EU at 37°C and fixed. For the bottom panels, cells were incubated with EU for 6 hours at 37°C, then transferred to medium without EU and stressed for 1 h at 45.5°C. Following heat shock, samples were immediately fixed and processed to visualize EU incorporation. Size bar is 20 μm.
Click here for file
Different quantification methods give comparable results
In light of the different strategies that can quantify pixel intensities in nucleoli, it was important to compare these methods. In Figure
Figure 9
Comparison of methods for the quantification of fluorescence associated with nucleoli
Comparison of methods for the quantification of fluorescence associated with nucleoli. Nucleoli were identified with the DAPI image as indicated in the figure. The different filter operations, which were performed to reduce noise and false positives, are listed on top of each panel. (a) Nucleoli were identified with the DAPI image;
Automated processing of images
For the methods described here, the identification of nucleoli and measurement of nucleolar pixel intensities require several operations. These include the processing of multiple images which are generated during the analysis. As an example, Additional file
Additional file 5
Quantification of nucleolar fluorescence with the
Click here for file
The repeated application of a large number of steps can be tedious and time-consuming. However, the work load can be reduced by automated processing of the images. Once the proper steps for image analysis have been defined (Additional file
Compatibility with high throughput screening technology
High throughput screening (HTS) technology is a powerful tool to explore different aspects of cell biology, and it can be expected that research on the nucleolus will benefit from the applications of HTS technology. It was therefore important to test the suitability of our protocols for automated HTS platforms. HTS applications have to overcome the limitation that the majority of high throughput imaging systems is equipped with widefield microscopes. To address this point, we applied the "detect light holes" protocol (Method 5) to measure nucleolar fluorescence intensities for fibrillarin in images acquired with HTS technology. The protocol was applied to the probe image, thus it was not necessary to use the DAPI image as a reference. For the results depicted in Figure
Figure 10
Analysis of nucleoli with high throughput screening technology
Analysis of nucleoli with high throughput screening technology. (a) Original images of HeLa cells stained with DAPI (blue) and anti-fibrillarin antibodies (red) were acquired with a high throughput imaging system equipped with a widefield optical imaging unit. Cells were incubated with the drugs indicated or the vehicle only (water for cycloheximide, DMSO for all other samples) and processed for indirect immunofluorescence as described in the Methods section. (b-e) Following correction for background, the probe image was analyzed for the presence of nucleoli, which were defined as bright holes that are between 2 and 5 μm2 in size and contain pixel intensities larger than the user-defined threshold. The total number of cells was determined with the DAPI image. It should be noted that the background correction and data analysis were performed in a fully automated fashion without visual inspection of the images. In parts b-e results for three independent experiments are depicted as average + STDEV. (b) For each condition the total number of nucleoli was divided by the number of cells; the results were normalized to DMSO-treated samples. (c) The area stained with anti-fibrillarin antibodies was used to evaluate the effect of different compounds. The threshold was set at 4 μm2; and the percentage of nucleoli below this threshold was determined for each treatment. Several of the drugs increased the percentage of nucleoli that are smaller than 4 μm2. (d, e) The effects of drugs on the pixel intensity (d) and nucleolar area (e) were measured. Statistically significant differences for drugs delivered in DMSO were determined by One-way ANOVA. Data obtained for water and cycloheximide were compared by Student's
Two to four regions were visited in each well; the probe image was corrected for background fluorescence and analyzed for "light holes". Light holes were identified as regions that are 2-5 μm2 in size and show fluorescence intensities above a user-defined threshold. Each image was scored for compartments that fit these constraints. It should be emphasized that the background correction and data analyses were performed automatically on the whole set of images without visual inspection of the segmentation. However, the correctness of the segmentation was verified for several randomly chosen images.
The total number of cells was determined with the DAPI image, and the ratio of nucleoli/cell was calculated (Figure
To simplify the analysis, we scored changes to the nucleolar compartment by setting a threshold for the nucleolar area at 4 μm2. Figure
A more detailed examination demonstrates the drug-induced changes to the fluorescence intensity and nucleolar area as observed with fibrillarin (Figure
We further evaluated whether our assay is robust enough to be employed in HTS experiments. Actinomycin D is a well established drug to analyze nucleoli, whereas compounds like DRB or roscovitine are known to affect nucleolar as well as other activities. Therefore, we employed actinomycin D as the reference to calculate the Z factor for our assay. Following published protocols
Figure 11
Determination of the Z factor for HTS
Determination of the Z factor for HTS. HTS conditions described in Figure 10 were used to determine the Z factor of the assay. HeLa cells were incubated with the vehicle DMSO or actinomycin D (Act D). Cells were fixed and fibrillarin was visualized by indirect immunofluorescent staining. Images were acquired and processed as for Figure 10, and the percentage of nucleoli that are smaller than 4 μm2 was measured. The values for average + STDEV, obtained for three independent experiments, are depicted. On the basis of these results a Z factor of 0.57 was calculated.
It should be noted that with the experimental setting described for Figure
Given the complex and dynamic composition of the nucleolar proteome
Signals that can be quantified with the presented techniques go beyond those derived from proteins. We show that our protocols can be easily applied to RNAs or biological activities that are located in nucleoli, as exemplified by the transcription of rRNA genes. Collectively, our methods provide a multi-disciplinary approach that generates information not only at the level of single cells or single nucleoli, but also for large populations of cells. One of the benefits of the protocols designed by us is their compatibility with state-of-the-art fluorescence microscopy. We provide proof-of-principle that these procedures can be combined with high throughput image acquisition using HTS automated platforms. Developing these methods sets the stage to quantify nucleolar components and activities for numerous samples and experimental conditions. Together with the growing amount of information emerging for the nucleolar proteome, methods in quantitative microscopy as they are described here can be expected to produce new insights into the complex biological functions that are orchestrated by the nucleolus.
Growth of cells and heat shock
HeLa S3 cells were grown on poly-L-lysine coated cover slips and exposed to heat shock as previously described
Immunofluorescence and confocal microscopy
Fixation of cells, permeabilization and incubation with antibodies followed published procedures
Confocal microscopy was carried out essentially as described
Measurement of transcription associated with nucleoli
RNA was labeled by incorporation of 5-ethynyluridine (EU) using click chemistry as described
High throughput screening and image acquisition
For experiments shown in Figure
Software and basic operations
All images were analyzed with MetaXpress software (MDS Analytical Technologies). The procedures described here rely on several software operations that are used in different combinations. The selection and purpose of different filters is summarized in Figure
(1) Detection and demarcation of nucleoli
Detect dark holes
The morphology filter
Detect light holes
The morphology filter
Add function
This operation combines two images by adding their pixel intensities for pixels that are in corresponding positions. The
(2) Reduction of noise and false positives
In addition to nucleoli, other regions in the nucleus may also be recognized by the
Median filter
Erode/dilate filters
As an alternative to the
Additional file 6
Comparison of the
Click here for file
Additional file
(3) Segmentation and quantification of fluorescence intensities in nucleoli
The median or erode/dilate filter images are analyzed with a defined set of parameters; they include size constraints and fluorescence intensity above local background. Based on this information, the software module creates segments that colocalize with nucleoli. Nucleolar fluorescence is then quantified by measuring pixel intensities in the probe image at the positions defined by segments.
Statistical correction (Background correction)
Prior to the measurement of pixel intensities in the nucleolus, the probe image has to be corrected for the contribution of background fluorescence. This can be achieved by the statistical correction function. Statistical correction subtracts from each pixel in the image the average intensity value of a selected region which does not contain cells. For a HTS setting a background image is automatically subtracted from all the images analyzed.
To measure fluorescence signals in nucleoli and nuclei, images for at least 20 cells were acquired by confocal microscopy for each of the different conditions. For HTS experiments, a minimum of 51 cells were analyzed per data point for each of the three independent experiments. For quantification, fluorescence signals were integrated over the entire nucleolus and nucleus. Results are shown as means and STDEV. Significant differences between control and stressed cells were identified with Student's
List of abbreviations
Act D: actinomycin D; CHX: cycloheximide; DAPI: 4', 6-Diamidino-2-phenylindole; DRB: 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole; EU: 5-ethynyluridine; Meth: methotrexate; Pol II: RNA polymerase II; Rosc: roscovitine.
Authors' contributions
MK developed all of the protocols; MK and PB performed the experiments. MK and US wrote the manuscript. All authors read and approved the final manuscript.
We are grateful to C. M. Brown, M. Guevara, H. Sampson, A. Spurmanis and K. Teske for their continuous support and helpful discussions. This research was supported by grants from CIHR, NSERC and HSFQ to US. MK was a recipient of a fellowship from the Heart and Stroke Foundation of Canada and a postdoctoral fellowship from McGill University. PB was supported by a fellowship from FRSQ/Heart and Stroke Foundation of Canada.
New insights into nucleolar architecture and activity
The moving parts of the nucleolus
Cellular stress and nucleolar function
Nucleolus: from structure to dynamics
The multifunctional nucleolus
Nucleolus: the fascinating nuclear body
RNA viruses, hijacking the dynamic nucleolus
In search of nonribosomal nucleolar protein function and regulation
Nucleolar targeting: the hub of the matter
Chromatin, linking structure and function in the nucleolus
Nucleolar Adaptation in Human Cancer
What the nucleolus says to a tumour pathologist
Involvement of the nucleolus in replication of human viruses
Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis
Nucleolar proteome dynamics
Nucleolar components involved in ribosome biogenesis cycle between the nucleolus and nucleoplasm in interphase cells
High mobility of proteins in the mammalian cell nucleus
NOPdb, Nucleolar Proteome Database-2008 update
Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions
Functional proteomic analysis of human nucleolus
Deciphering the human nucleolar proteome
Arabidopsis nucleolar protein database (AtNoPDB)
Analysis of signaling events by combining high-throughput screening technology with computer-based image analysis
Dissection of the molecular mechanisms that control the nuclear accumulation of transport factors importin-α and CAS in stressed cells
Chaperones and multitasking proteins in the nucleolus: networking together for survival?
Stress inhibits nucleocytoplasmic shuttling of heat shock protein hsc70
Exploring RNA transcription and turnover
Chemotherapeutic drugs inhibit ribosome biogenesis at various levels
Simple Statistical Parameter for use in the evaluation of high throughput screening assays
Proteomic analysis of the nucleolus in adenovirus-infected cells
Quantitative proteomics using stable isotope labeling with amino acids in cell culture (SILAC) reveals changes in the cytoplasmic, nuclear and nucleolar proteomes in Vero cells infected with the coronavirus infectious bronchitis virus


text:
- Description
Add style to any dining area with this round counter height glass table with a striking, curved table base. Finished in gray with matching ivory leatherette chairs.
1 Round Counter Height Table
Round Counter Height Table, 48"DIA X 36 3/4"H
More from this collection: Manhattan III
- Additional Information
Additional Information
SKU 13794555 MPN CM3710GY-PT GTIN UPC 841403113085,841403113092,841403113108 Brand Furniture of America Collection Manhattan III Assemble Length No Assemble Width No Assemble Height No Assembly Required Yes Material Wood Finish No Styles Contemporary Shapes No Shipping Time 1-2 Weeks Free Shipping No
- Reviews
Disclaimer: Pictures displayed may include optional products not included in the price. Computer monitors often display color variations. Color swatches are shown if alternative colors are available. Please call us or email us if you have any questions!


text:
Download free Chubby housewife enjoys sex with her boss from Section: Indian sex videos & Category: Adult videos villa. Filetype: Chubby girls full sex videos & Size: 18191.54 kb
¤ Main menu
¤Related Site Links
Get "Chubby housewife enjoys sex with her boss" at your phone.
File size : [18191.54 kb]
Loading the player...
Similar Downloads
Booby chubby lady gets her pussy drilled by neighbor
Chubby housewife enjoys sex with her boss in hubbys absence
Village chubby aunty much interested in doggy style sex vide
Son fucking his hot mom chubby mother fucked by son on floo
Cute chubby goth enjoys a fuck and a facial video
Hot chubby matures and pregnant women gives hard bj to group


text:
Advertisement picturing is by far one of the most competing forms of picture taking. This is for the most part due to the fact that you can get far more business than in any else feature of photographic industry.
Every day companies pay trillions of dollars on advertisement; near one objective - to trade their merchandise. Companies poorness to archer the student that their goods is sunday-go-to-meeting - so you must be able to ignitor this.
The photographers toil essential be to the highest technical element and he/she must have the capacity to instigate a masterpiece from something unrefined. Close sufficient is merely not good decent. Close ample will not vend a service.Post ads:
Extra Pockets School Bag Traveling Bicycle Backpack Hiking / Andrew Philips Bus Card Case / Heart Pattern 3pc Rectangular Travel Set Beige / Shopper Bag Saltire Heart I Love Scotland Navy / RealTree Camo Men's Leather Trim Key Ring Gift Set / Seeline Eyewear Sunglasses Black Grey / BLT-YLW - Reversible Belt - 30 inch - 60 inch / Icon Eyewear Sunglasses Sunglasses / Steve Madden Women's/Girl's No Show Socks 6 Pair -Size: / Bloom - Grey - Make Up Bag / Wash Bag by Nicky James / Somatic PLEASURE Womens Oversized Sunglasses - All Colors / Tabbi Socks Super Loose Socks - Maroon / 12 Pairs Womens Cotton Rich Black Socks. / Women Backpacks of Multiplicate Colors / ilovemyreadingglasses Fashion Reading Glasses - Cool Brown / Umbrella child "Betty Boop" transparent red. / Fancy belt "Kika" red. / BLT-SKY - Reversible Belt - 30 inch - 60 inch / Absolute Outdoor 15082 Polywool Socks Black M
To become an advertisement creative person one essential basic take in the market and comprehend what the client is looking for. The unsurpassable way of recognition this is select up a public press and expression at the ads. Study each figurine. Study the similes you see on billboards or metaphors that are used to hype pictures.
Most environs of flier picturing can be classed as nonmoving existence picturing. So if you are curious in attractive metaphors for the personal ad flea market your early job is to set up your own quarters workplace. This can be a marvellous put down to practice.
Every entity in your hole can be photographed. Start off next to a few eyeglasses. But evoke you are not provoking to kind the spectacles exterior good - you are exasperating to provide them.Post ads:
DGK Haters Black With Clear Lens Shades / Marilyn Vintage Metal Frame Coin Purse / BLT-RYL - Reversible Belt - 30 inch - 60 inch / 1.5inch Celtic Thistle Trouser Belt / BOBSTER TEXAS SUNGLASS CLR LENS CLOSED CELL FOAM EZTX001C / Makeup kit "Fuchsia" rainbow. / BLT-MST - Reversible Belt - 30 inch - 60 inch / Umbrella child "Maya L'abeille" yellow green. / Hello Kitty Plaid Cosmetic Case / Black Mock Croc Snap Close Wallet - Vegan Friendly / Rasta Hemp Belt - Black RGY W03S66F / About Color Pinic Scarf / 1.5inch Celtic Stag Trouser Belt / Seeline Eyewear Sunglasses Silver Grey Gradient / Umbrella "Singing In The Rain" multicoloured. / Ironstone Quilted Cotton Small Wallet / Seeline Eyewear Sunglasses Gold Grey / Fluffy co. Low Rider Eco Mini Wallets - Brown / Chaps Men's 3-pk. Cotton Blend Dress Socks
The second entity you demand is a man-sized data formatting camera. Buy a field of the unsurpassed lenses. Slide motion picture is your first superior for want ad picturing. The purchaser may poverty to tap up the imagery to a hoarding mass.
The furthermost principal point once starting off is creating a case of your toil. This can be transmitted to selling agencies or can be shown direct to a company. Always have a few copies on standby. Also set up a web folio. On-line advertisement is budding at a striking rate. Your case should besides belong of a antithetical extent of descriptions.
If you are rational of victimization an trailer agency, get their guidelines from them. These should be massively helpful to you in benevolent the flea market. Dealing with a symptomless habitual bureau should be intensely worthwhile for you if your pictorial representation is to par.
Advertisement can be seen everywhere - and roughly 80 per centum of all ads use picture taking. Most advertising these life consists of a few spoken language and a impressive work of art that should attract the viewer's publicity.


text:
Fitness club replaces dues with charitable fundraising
Back in 2008 we covered Plus 3 Network, the California-based site that lets fitness enthusiasts raise funds for charity with each workout. Picking up on a similar idea, Can Too is an Australian fitness club that provides professional training sessions and support in exchange for charitable fundraising.
Spotted another good idea???
Join our network and earn rewards


text:
President Obama is getting a boost as commander in chief from the stunning news that Osama bin Laden was killed by U.S. forces.
But does George W. Bush, Obama's Republican predecessor, deserve some political credit, too?
As with lots of things in a sharply divided Congress, the answer is tinged with partisanship.
Less than 25% of Republicans in the U.S. House who have issued a news release about bin Laden's death commended Obama, while less than 3% of Democrats in the House gave a nod to Bush for setting things in motion after the 9/11 attacks, according to an analysis out today from the University of Minnesota's Smart Politics blog.
"Members of Congress were not united in the thanks and praise they gave for the landmark successful mission against the FBI's No. 1 most wanted terrorist," Smart Politics said.
In its unique analysis, Smart Politics studied the press releases and statements made by House members since Obama's announcement Sunday night that bin Laden was dead. It found that 87% of House members issued a press release on their official websites or made a statement about bin Laden via Facebook or Twitter.
Some findings:
Catalina Camia leads the OnPolitics online community and has been at USA TODAY since 2005. She has been a reporter or editor covering politics and Congress for two decades, including stints at The Dallas Morning News and Congressional Quarterly. Follow her at @USATOnPolitics.
USA TODAY's Jackie Kucinich (@jfkucinich) and Fredreka Schouten (@fschouten) also contribute to the OnPolitics blog.


text:
MCM Heritage Boston Purse (Small)
$ 830.00
The refined timeless tote shape is a distinguished collectible, timeless in poise and chic in style. Structured to perfection, this season's introduce an allover signature logo print that is suitable for multiple occasions.
- Ships from Atlanta with MCM warranty documentation and dust bag
- Double handles; detachable adjustable crossbody strap
- 9.2 W x 5.9 H x 5 D


text:
Hime wrote:I don't think I've even seen Coke Life. Is it sold in cans?
Used to come in all varieties bar glass bottles.
Talking of which, Coke Zero, Pepsi and Pepsi Max all come in glass bottles but I only ever see them individually in certain newsagents. Never see them in any of the supermarkets. It does look a hell of a lot better.


text:
Best Of :: Shopping & Services
Drive-thru liquor shops can be singularly seedy affairs — and God knows East Phoenix, from Indian School to McDowell Road, is full of plenty of dubious examples. But even though Tower Liquors falls smack dab in the middle of this milieu of grungy alcoholic functionality, it's truly a gem: a spotlessly clean, perfectly organized shop that offers everything from Jose Cuervo to pickled eggs. The icing on the cake? The building is a Mid-Century Modernist's dream, with the kind of design details that inspired haughty hotspots like the Vig and the Parlor. During the day, it may not look like much; at night, we dare you to drive past its bright neon sign and not feel the gemutlichkeit. All that and cheap tequila, too.
In the summer of 2009, husband-and-wife team Liam James T. Murtagh and Emily Spetrino-Murtagh took over the old Art One space and opened this novelty shop that fuses two sweet things: vinyl records and candy. The Murtaghs, who play in a number of punk and ska bands, focus their records on those two genres as well as new wave. And there are all sorts of sugar-loaded confections, ranging from jars of eye-popping candy to edible mustaches. The space doubles as the couple's living quarters, so they're open only in the evening when they get home from their day jobs. BTW, don't be alarmed to see the Murtaghs' 2-year-old son, Iggy, running around the shop and being crazy playful. He won't bite.
Tucked behind the yummy Paisley Violin Café is this retail co-op that's housed in totally adorable structures that weren't so adorable at one time: The World War II-era cottages once held German and Italian prisoners of war. However, you won't feel anything weird or creepy when visiting the buildings that were moved from 19th Avenue and McDowell Road and remodeled by Gina and Derrick Suarez. Instead, you may very well experience local-art heaven in shops such as Urban Art Florist and Wicked Wear Studio and Gallery. The collective often unites to hold special events like the Paisley Town Art Festival as well as special dealios during Art Detour.
We shop. A lot. Which is why we figured we'd easily find a new winner in the "Best Boutique" category this year. No go. In good faith we simply can't stray from our favorite, Frances. Georganne Bryant doesn't just run the cutest boutique in town — she's started a cottage industry when it comes to community support. She gives well-earned shout-outs to other local businesses on her blog (www.francesblog.tumblr.com), supports the indie movement in all sorts of creative ways (from sponsoring movies like Handmade Nation to printing T-shirts that say, "Love Phoenix or Leave Phoenix") and she's the first to credit someone else with a good idea. But usually, truth be told, it's Georganne who has the good ideas. If you don't care about all that community building, buy-local hoo-ha, come to Frances because Georganne has good taste, along with good ideas. She stocks the sweetest collection of clothing, housewares, jewelry, and paper goods this town has ever seen. Now you can shop on Frances' website — and our pocketbook (super-cute, purchased at Frances) is really doomed. But be sure to sign up for Georganne's mailing list, because twice a year, she has a really good sale. Just don't go telling the whole world or anything, 'kay?
We'll follow Bunky wherever it goes. And we have. Owner Rachel Richards-Malloy has kept us on our toes since she opened — this is her third location. Today, her teeny shop is located across the street from the Phoenix Art Museum and shares a home with Matt Pool's Giant Coffee in the Merz building. The store is filled with designer clothing and accessories and is cleverly arranged to fit even more inventory than she had before — Bunky now carries books and housewares. None other than Hayes McNeil from Plus Minus Studio designed her modular space. The walls are lined with salvaged wood (you can even spy an old Pepsi can nailed into one of the wood panels). Bunky's got designer threads in a designer space with designer coffee, all in one spot? Oh, man, we are so there.
This cute girl named Olive needed a place to shack up, and turns out Paris Envy had some room upstairs, so a partnership of sorts was born. And we're so glad. This trend of sharing space is a wonderful byproduct of a slow economy, and we hope it keeps classy indie businesses like Paris Envy and Olive in Paris (the latest project from the ladies who brought us the sadly short-lived Olive Annie on Seventh Street) going strong. We drooled over a pair of vintage lamps at Paris Envy, a housewares shop with a shabby-chic French (obviously) vibe, and upstairs, we debated over a piece of handmade jewelry or a funky multi-colored belt. Both stores have so much style and personality and . . . sorry, but you'll have to excuse us because we need to go back and see whether those lamps are still there. Be right back!


text:
Come on out, witches and wizards of Hogwarts. Harry Potter is dead! And now… now we can build a new world. A world without the threat of the Mudbloods!
This guy had an ego. But there was no mistaking Harry's body.
"NO!" Darion cried. Even though I knew he was gone, I was still beyond tears. I wanted to run out, to seize Harry, to hold him tight and never let him go, but Amber held me back.
"Not yet… we have to wait until it will do the most good… at least let someone kill the snake."
Now, who will join me? Who shall join me in the reconstruction?
Only one man was prepared to step forward. Neville Longbottom looked at Riddle courageously.
One? Well, I hoped for better…
"I don't give a flying lion what you have to say, Voldemort. I was given one mission, and one mission only. I, Neville Longbottom, leader of Dumbledore's Army in Harry's absence, hereby condemn you and your pet snake to the darkest corner of hell!" Neville roared in outrage. He reached into the Sorting Hat I had subtly rolled next to him without thinking, and drew the Sword of Gryffindor with a sharp wring. He levelled it, and sliced.
NNNNNNNNNNNNNNNNNOOOOOOOOOOOOOOOOOOO! Riddle's scream was heard for miles, despite being mostly hiss. As Nagini's head spiralled away from the body. At that instant, Harry's body vanished.
"Ginny, it's time. We have to-"
"Find Harry's body. He might be alive!"
As the fight was moved into the Great Hall, the Death Eaters were all but cornered by the oncoming hordes of allies of many different species, affiliation, and… yeah, everybody and their mother showed up. However, the two biggest threats, Bellatrix and Riddle, were not easily sedated. A group of professors holding off Riddle, we were searching for Harry when she got in our way.
"Ginny… and Amber, too? My word… this'll make for a fun show!" Bellatrix, no doubt completely off the deep end, had shown up, and we were pretty much locked into battle.
Amber started off by launching several different spells, each one missing Bellatrix by about a hair. Bellatrix panicked, and shot a jet right back at her. She blocked it, and I took up the fight.
As soon as I gave it a shot, her first jet narrowly missed me.
"Fear tactics didn't work? Suppose she really is insane," Amber remarked, before she and I were pushed aside by Mum.
"Never, ever do that again, Lestrange!" she taunted, before launching a bedazzling series of hexes. This time, the barrage landed, and Bellatrix was pissed.
"This'll be the doom of yet another Weasley… and then where are the parents? Who'll look after ickle Ginnikins after Mommy, Daddy and Harry are all gone?"
"Never. Threaten. Us. Again!" Mum roared, and shot a fiery plume. I shot a grassy one, and Amber a watery one. All three collided in mid-air, and hit Bellatrix straight in the chest, shattering her into a thousand fragments.
"Everyone's sick of that scream already, Thomas!" Harry chuckled, removing the Invisibility Cloak.
"HARRY!" I cheered, throwing my arms around him, until Amber prodded me off. We all stood beside Harry, facing Riddle.
How did you not die?
"Well, long story short, you killed my mother, stole my blood, I was one of your Horcruxes, and the Elder Wand isn't yours. It's mine."
But Snape killed-
"Yes, Snape did kill. But the murder would not have seized the Elder Wand even if Dumbledore still had it. It belonged to Draco, who was the only one to take the wand by force. And then I was the first to overpower him. Heh heh… I suppose we could have an old fashioned wand fight now, could we?"
Your death will mark my ascent into godhood!
"You aim far too high, Mr Riddle. Nebulosus Terrenus!" Amber said, flourishing her wand. Four pink jets hit the four corners of the room, encasing our arena in a pink fog. The spectators did not show up, probably to avoid unnecessary damage.
Amber began the fight by turning into her eagle form. She cawed, and started diving, pecking Riddle. Me and Harry saw Riddle prepare a counter, and interrupted him. Harry repelled Amber from the fight, threw his and Riddle's wands to one side, and began to wrestle with the snake.
So this is how Muggles fight? Pathetic. They'll be easy prey! He redoubled his efforts, pulling Riddle's arm around behind his back.
"Now what do you think?" he asked, giving him a solid kick. "They work for and against bullies well." I threw him his wand, and prepared a finishing blow.
"Alakazam!" Amber called, somehow turning into a human well. Harry looked at her oddly.
"Alakazam? You use a Muggle spell, with no idea what it might magically do?" he asked. Tom's body was encased in thick, pink bands, obviously causing him mental anguish. He roared, and his body sunk under the earth with an odd sound.
"Now what?" I asked. I shouldn't have. A white mist emerged from where Riddle last stood, and merged with Amber. She coughed, hacked and wheezed, doubling over.
"Ah! Ginny, what happened?" Amber panicked.
"Hold still, Muggle girl!" I snarled. The diary dropped from my hands.
"Wait, didn't Harry have this?"
"Amber, DON'T TOUCH-" I started. Amber opened the diary, and a golden light engulfed her.
"…that," I finished lamely, falling and registering no more.
The sight of Amber, vulnerable as she was in the Chamber of Secrets, no longer repulsed me after the pain was over. Cackling wildly was her replacement… that possessed Amber that still had the indecency to flirt with Harry.
"Now, where were we? Oh, that's right. I remember! I was about to kill you!" Amber taunted mercilessly. I was still laying down from the shock.
"Now Harry, this'll be quick. Avada Kedavra!" she cried, and Harry dodged the bullet like a pro.
"Hm… this will be far more of a challenge than I expected." Psychically, Riddle picked up the Elder Wand, and drew it to his hand.
"How 'bout a dual-wield?"
"Is that even possible?"
"No, but I'll do it anyway!"
Have you ever tried fighting someone with two wands? Yeah, even with the disadvantage of having to look at two targets at once, Riddle was easily kicking our butts. If only he knew to not kick our butts…
"Avada Kedavra!" he cried, aiming directly at me. Harry knocked me to one side, taking the bullet to the arm. A second shot, and Harry was well and truly gone.
"…You just messed with the wrong girl, Thomas Marvolo Riddle. Quodest Radior!" My Hyper Hex flowered from my wand at such an incredible speed, Riddle was pulled along by the blast, until he hit the far wall and sunk to the the floor. Amber looked up at me, and smiled. Her head fell to the floor, gone.
"…My goddess… What have I done?" I panicked briefly, before fainting due to use of the Hyper Hex.
I stirred, moaning as I tossed and turned.
"Good morning, Ginny. Sleep well?" Mum asked. I sat bolt upright.
"What happened?"
"No idea, Ginny. Amber cast some jinx that hid all four of you from view, and when you were back in the real world, you were fainted over Harry, Amber looked like she had been crushed against a wall, and we couldn't find You-Know-Who's body…"
"I'm piecing it together… wait, where are Harry and Amber?"
"Right over there. We're in the Hospital Wing, darling." I got off the bed, and looked at the two. Amber was on life-support, with Darion holding her hand. Harry was merely getting rest…
Quit fooling yourself. They're dead, and nothing you can do can change that simple fact.
…Correct. There was nothing I could do.
"Stop the medical treatment!" I found myself saying.
"Why, Ginny. They need this treatment to survive!"
"That's the thing… they didn't. Neither of them made it out of the Misty Terrain."
Shortly after Harry and Amber's cremation, I entered my dormitory, to find more stuff than was mine.
"Harry and Amber wanted you to inherit their stuff should they have fallen…" Hermione said, from one of the other beds. I nodded vaguely, and spotted a particularly spacious trunk belonging to Amber. I sorted all the stuff into that trunk, or to be given to someone who could make use of it.
"You're so lucky," Hermione muttered.
"Why?" I countered, tossing one of Harry's shirts in a trunk for Darion.
"Harry and Amber got a funeral. We barely buried Ron properly."
"Look, Hermione, that wasn't our fault. The wizarding world would marry Harry if I wasn't dating him… if I weren't dating him. And Amber's been on more of a spiritual quest, to master more spells than I think exist. Ron… well… at the time, no one really gave a damn about him."
"Ginny… fine. It's not like anyone gave me any attention."
"Hermione, are you OK?"
"Oh, just being torn apart inside by nearly every single negative emotion known to Muggle or wizard alike. Not like you'd care, though. You're just waiting to lull Harry to sleep, or find one of Amber's precious uber spells."
"Hermione… I'm sorry, I didn't know!"
"Yeah… you didn't even bother trying to give me the time of day, Lily! Admit it."
"Hermione, please!"
"I've had it! There just isn't anywhere for a sane person to go anymore! I… really need to talk to him…" Hermione opened the window, and sat on the sill.
"Hermione, we can talk. Now that Harry's gone…"
"Yeah, sure. Now that Harry's gone, you have all the time in the world to talk. Not before," Hermione put her legs on the other side of the sill. I knew instantly what would happen next.
"Hermione, GIVE THIS A SECOND THOUGHT!"
"Too late for me, Ginerva. Make sure I get a funeral." Hermione pushed. She fell off Gryffindor Tower. I very nearly jumped off myself trying to catch her, but it was too late. I chose not to see the splatter. I turned to my inheritance, and absentmindedly grabbed another thing.
Maybe I have been taking Hermione for granted… her and Ron. If only I could make it up…
Harry and Amber are probably doing it. And was Harry going to propose to you?
I looked in the palm of my hand. Indeed, it was a beautiful ring, encased within its own box. Smiling to myself, I put it on, and continued to sort this stuff out. I didn't want to bring up Hermione's suicide yet…
Yeah, maybe I finished off on a bad note. Perhaps someday I can 'fix' this.
Next time, the revelation of my next projects... or maybe even my next 'seven'. Hm?
...The hints are not working, are they?
Two must fall to the hand of one..


text:
It only takes a minute to get started. Fill out our simple sign up form and login to the website.
This step is optional but will seriously multiply your chances of getting noticed by other people on the website. Write a little about yourself, your pasttimes and upload your nicest photo of yourself.
Use our website's advanced search to find members who match your needs. Or just scan through listings and find people that seem interesting.
Located someone who takes your fancy? Send a letter to them or just a wink and see what they say. With a little luck you'll be on your way to uncover that special someone for you.
You won't regret it


text:
Cup fever: Brands court football after cricket
With the domestic 20-20 cricket tournament ending, corporate sponsors are doffing cricketing gear and donning soccer boots as the FIFA soccer world cup looms.business Updated: Jun 03, 2014 00:16 IST
With the domestic 20-20 cricket tournament ending, corporate sponsors are doffing cricketing gear and donning soccer boots as the FIFA soccer world cup looms.
Brands such as Hero MotoCorp, Lava Mobiles, Microsoft, Nokia and Adidas are leading the race to woo soccer fans. "The traction of advertisers during soccer matches has gone up phenomenally. With the world cup, brands reach out to a set of audience that may not be hooked during cricket matches," said Rohit Gupta, president, ad sales, Multi Screen Media, which owns Sony SIX, the new official broadcaster of FIFA.
Coca-Cola, a fixture at cricketing events, is switching gear. "Our endeavour is to forge a special connect with football fans," said Debabrata Mukherjee, vice-president-marketing, Coca Cola India and South West Asia.
Adidas, which signed Virat Kohli as its brand endorser last year, has historic ties with soccer. "We will leverage our strong on-field visibility at World Cup," said Tushar Goculdas, brand director, Adidas India.
Despite just 900 seconds available, against 2,400 seconds during the T20 cricket matches, the rates have not fallen from the last edition. "The channel is selling slots for Rs 2.5 lakh per 10 seconds, the same as ESPN charged during last FIFA. Soccer has re-confirmed its position as the second most attractive sport in India," said Navin Khemka, media buyer, ZenithOptimedia.
Brands spent over Rs 5 lakh (per 10 seconds) during initial cricket league matches. During the cricket world cup, rates soar to Rs 6-8 lakh.


text:
Will Face the State correct its headline linking to bogus blog entry, now that blog has acknowledged its own falsehood?
As Colorado Media Matters noted [1], the "news" website Face the State [2] falsely asserted [3] on October 17 that the online political daily news site Colorado Confidential [4] "yank[ed]" a Jim Spencer [5] diary entry critical of the campaign for Denver Mayor John Hickenlooper's Better Denver [6] tax and bond package, and linked to a conservative blog that originally made the claim. Although the blog, Wren's eJournal [7], has acknowledged that its item was inaccurate, Face the State as of October 18 had not corrected its false headline -- "Tim Gill-Backed Website Yanks Critical Article Against A-I" -- linking to the Wren's eJournal entry.
Wren's eJournal stated [8] on October 17 that Spencer's October 10 diary entry on Colorado Confidential was "no where to be seen on that website." In fact, consistent with Colorado Confidential's practice of featuring its most recent entries on its home page, Spencer's week-old diary [9] was moved off of the home page but remains live elsewhere on the website.
On October 17, Wren's eJournal acknowledged [10] the error and posted the following in response to a comment [11] from Colorado Confidential managing editor Wendy Norris pointing out the falsehood and the fact that Spencer's column still was available:
I just read Colorado Confidential's response to my report of not being able to find Jim Spencer's very interesting and informative article on A thru I. Their response in full is in the comments to my original post (see below). They seem to think an appology is necessary; if anything it seems to me they should be a bit humble about making the article next to impossible to find. Read Spencer's article now if you haven't already, here's how:
It can ... be accessed via a search on Colorado Confidential [12] by selecting "diary" and typing "Hickenlooper" as the keyword.
As of October 18, Face the State had not corrected its October 17 headline, which remains posted in its original form on Face the State's archives:
Where'd Jim Spencer's Column Go?
Tim Gill-Backed Website Yanks Critical Article Against A-I [13]
Now that Wren's eJournal has acknowledged its error, will Face the State do the same?


text:
If you haven't seen Charles Ferguson's buzzed-about 2007 documentary, "No End in Sight," and you wanted to, you're in luck: You can now watch the film on YouTube in its entirety. Thanks, Mr. Ferguson!
Posted on Jul 27, 2017
A Progressive Journal of News and Opinion
Publisher Zuade Kaufman | Editor Robert Scheer
About Us | Contact Us | Advertise or Partner with Truthdig | User Agreement | Privacy Policy | Comment Policy
© 2017 Truthdig, LLC. All rights reserved.
Website development by Hop Studios


text:
Status: Closed
Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
Psychotherapist who works with individuals, couples and families. Based in Corte Madera, CA.
Sanford Ellen Mft is a Psychologist office located in Corte Madera, CA. A psychologist focuses on the evaluation, prevention, diagnosis, and treatment of mental, emotional and behavioral health issues. A clinical psychologist uses psychotherapy and other counseling skills to improve emotional and mental health.
Please call Sanford Ellen Mft at (415) 945-3200 to schedule an appointment in Corte...
Be the first YellowBot user to add photos or videos of Sanford Ellen Mft
Teen Pathways is located at 100 Tamal Plz, Corte Madera, CA. This business specializes in Social Services and Business Organizations.
Since 2007 the company has been providing Educational Consultant.
If you don't see your business listed on YellowBot, please add your business listing.
YellowBot wants to get your input! If you have a comment, find a bug or think of something neat we should do, let us know by emailing us.
© 2007-2017 Solfo, Inc. – All rights reserved


text:
Tags: mobile, New York City, NYC, Subway, Wi-Fi.
Posted 02/20/2014 at 5:39 PM
Posted 3 years ago
If you live in or around New York City, you'll soon be able to enjoy Wi-Fi service at the Grand Central Terminal, 34th St. Herald Square, and Bryant Park subway stations, as early as this summer.
William Bayne of Transit Wireless said "Hundreds of millions of new annual subway patrons will soon receive the benefits of having all the major wireless carriers in the subway stations. We are setting the stage for future innovations that will provide riders with an enhanced experience in the New York City subway system."
Transit Wireless expects to have Wi-Fi service enabled at 277 subway stations by 2107.
Service will still be unavailable in tunnels, however.
You must be logged in to post a comment.


text:
Jean Noisette, owner of Atlanta-based Dazzle Me Parties, who uniquely carved a niche with her brand, has garnered widespread attention from celebrities to cable networks like VH1. She has shared with us 10 goals every entrepreneur should set. CREATE BUSINESS
According to National Payroll Week's 2015 "Getting Paid In America" survey, 32% of Americans say it would be difficult to meet their current financial obligations if their next paycheck were delayed for a week, meaning the financial ground they're standing
Here's how a hobby can help you get a fresh perspective on business challenges. To my surprise and delight, I recently found motivation in unexpected ways. I'm someone who has always really enjoyed looking at other people's gardens. I literally stop
For most married couples, and pretty much anyone in a serious relationship, money issues are a huge part of what makes things work or fall apart. More often than not, the topic of finances is one that most dislike touching. According
Over the years, society has created "taboo" behaviors for women. Women are criticized for speaking up for what they want or need, not acting "ladylike" or showing off their talents. The risk for women does not lie in exhibiting these
With social media heavily integrated into our lives, it's easy to compare your career path to that of others. So whenever doubt seems to arrive on your doorstep, here are three tips you can rely on that will make you
Although it's usually the food that people pay the most attention to on the Christmas dinner table, you might want to learn how to dress up your table to make the room more festive. Of course, with the economy the
Before we cut into that juicy baked or fried turkey for Thanksgiving, many of us are already planning our Black Friday Shopping spree. Black Friday is regarded as the first day of Christmas shopping. Although it is not an official
When looking for that great mate, finding someone that is compatible in religion, personality, goals and yes, the bed is extremely important. However, financial compatibility is usually overlooked and often ignored. This is surprising since one of the main reasons
The holidays are upon us once again, and it's pop culture for people to dive head first into reckless spending so much so that at the end of the day, most of us are neck deep in debt. Most of


text:
Libntoh aims to be a user-friendly library to provide an easy way to perform defragmentation and reassembly of network/transport protocols. It is intended to provide a flexible, thread-safe, and highly configurable environment for the user. It performs IP defragmentation and TCP reassembly.
Graylog2 is an syslog implementation that stores logs in MongoDB. Messages are accepted via TCP or UDP. A Web interface allows you to view the log messages. It also implements GELF, the Graylog Extended Log Format, which is a simple JSON string that allows you to send long and structured syslog messages, which is very useful for customized application log monitoring.
Sxwd is a multi-purpose tool for dealing with xwd images. Xwd is a C++ class which was created by merging xwd.c and xwud.c from the X11R6 distribution and porting them to GNU C++. Sxwd is a command line wrapper to exercise the member functions of xwd. Sxwd was created to manipulate the colormaps of 8-bit PsuedoColor XWD images in the production of custom USGS topographical maps. A few additional features have been added.
vmodemd is a telnet server designed to run old DOS BBS systems and door games. It listens for incoming TCP connections on a defined port, does a minimal telnet handshake if needed, and then starts to proxy all data to a PTY which is bound to a dosemu virtual serial port. vmodemd supports any number of virtual modems, can display customized banners if all modems are busy, can terminate a serial app when a telnet connection is unexpectedly dropped, and run custom scripts before passing control to dosemu, allowing you to pass relevant data like incoming IP address to the DOS app.
A multiplatform, 3D space-combat MMORPG.
A GNU/Linux rescue CD that comes with Rescapp, a nice wizard that will guide you through your rescue tasks.


text:
The Hacienda Matapalo real estate project has changed hands, and those who gave money to the previous management are wondering if they are protected.
The change in ownership was contained in a .pdf file on the firm's Web site, addressed to "To Holders of Agreements to Purchase Home Sites and Condominiums at Hacienda Matapalo."
At the same time the local CBS television station in Miami aired a story interviewing residents there who had given the original management money in anticipation of construction, which has not taken place. A former employee of the former management was quoted saying that perhaps as many as 200 would-be purchasers sent $21 million to the firm, Pegasus Star Ltd., a subsidiary of the U.S.-based Centam Partners, LLC.
The announcement of the purchase was by Giovanni Varela, who was identified as the legal representative of Gestion Integrada De Residuos SRL, called GIR. Said the announcement: "Varela is one of the principals of INDECA, the premier architectural and design firm in Central America. However, we stress that GIR has no development arrangements or agreements in place at this time."
Apparently many of the would-be purchasers gave a down payment and continued to put money into what they thought was a trust account.
The announcement of the ownership change identified the previous operators and managers as David Matluck, Ed Sklar and Brian Albury.
The announcement also said that the Matapalo property is subject to a secured loan with a firm identified as UTA Capital LLC. The announcement said that Gestion Integrada De Residuos took over the management in lieu of foreclosure and that additional resources were provided.
Said the announcement:
"You can expect a new philosophy and different set of priorities moving forward. GIR is not a marketing company, nor a sales-driven organization. Do not anticipate the numerous emails, solicitations, marketing materials or various electronic updates to which you had become accustomed with the former owner. GIR is focusing squarely on the necessary steps to complete the infrastructure that will allow for lot closings and for vertical construction to begin.
"GIR has chosen to direct the bulk of its resources towards producing tangible results. GIR is effectively transitioning to the construction phase of development.
"In anticipation of taking ownership of Hacienda Matapalo, GIR had been systematically laying over the past several months the preliminary groundwork necessary to move the project forward, with funding support and encouragement so far from UTA Capital LLC. The long awaited HOA documents are now complete and filed with the proper governmental agencies.
"The individual surveys for the single-family home sites have been completed and we expect to receive the final governmental approvals for lot closings in August. In combination with those surveys, the other required preliminary steps to facilitate single-family home site registration for inclusion in the National Registry are also nearly complete.
"All of the crucial permits and approvals have been secured including, but not limited to, the water concession and sewage treatment plants. Hacienda Matapalo has continued to successfully pass all the necessary environmental inspections from Setena and Acopac, two important Costa Rican governmental agencies. In the second half of July you can expect to see work resume on infrastructure, the entrance way and the guardhouse."
Acopac is the Área de Conservación Pacífico Central, and Setena is the Secretaría Técnica Nacional Ambiental.
The announcement also said that some former employees of Pegasus, Michael Starkey and Nathan Posey, are being retained in the United States to serve as liaisons between the new owners and those who are purchasing property.
"In the coming months you can expect to be systematically contacted by Michael or Nathan as they will be assigned to assist the new owner in two very important specific tasks: (1) execution by prospective buyers of new purchase and sale agreements with GIR, and (2) determining which individuals who signed appropriate purchase option agreements with Pegasus are prepared to close and have the necessary financial resources to meet the closing obligations of the GIR agreements."
In short the announcement probably means that those who gave money to Pegasus have lost their cash because UTA Capital has taken over the property and that anyone who wants to do business will have to start all over with the new firm. To get their money they will have to start legal actions against Pegasus.
The 665-acre project was at Playa Matapalo, which is on the central Pacific coast between Quepos and Dominical. The Web site called Matapalo the No. 1 real estate development in Costa Rica.


text:
Browsing 2011 Atlanta Conference on Science and Innovation Policy by Subject "Market formation"
Now showing items 1-1 of 1
Drivers of innovation of biofuel ethanol. A comparative analysis between the U.S. and Brazil. (Georgia Institute of Technology, 2011-09-17)The United States remains dependent on energy sources derived from fossil fuels. The transportation system in particular is 98% dependent on oil. This overreliance on oil has raised concerns over the long term impact of ...