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The Major Envelope Glycoprotein of Murid Herpesvirus 4 Promotes Sexual Transmission.
Zeippen, Caroline ; ; Xiao, Xue et al
in Journal of Virology (2017), 91(13),
Gammaherpesviruses are important human and animal pathogens. Infection control has proven difficult because the key process of transmission is ill understood. Murid herpesvirus 4 (MuHV-4), a ... [more ▼]
Gammaherpesviruses are important human and animal pathogens. Infection control has proven difficult because the key process of transmission is ill understood. Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus of mice, is transmitted sexually. We show that this depends on the major virion envelope glycoprotein gp150. gp150 is redundant for host entry, and in vitro, it regulates rather than promotes cell binding. We show that gp150-deficient MuHV-4 reaches and replicates normally in the female genital tract after nasal infection but is poorly released from vaginal epithelial cells and fails to pass from the female to the male genital tract during sexual contact. Thus, we show that the regulation of virion binding is a key component of spontaneous gammaherpesvirus transmission.IMPORTANCE Gammaherpesviruses are responsible for many important diseases in both animals and humans. Some important aspects of their life cycle are still poorly understood. Key among these is viral transmission. Here we show that the major envelope glycoprotein of murid herpesvirus 4 functions not in entry or dissemination but in virion release to allow sexual transmission to new hosts. [less ▲]Detailed reference viewed: 12 (3 ULg)
Conserved Fever Pathways across Vertebrates: A Herpesvirus Expressed Decoy TNF-alpha Receptor Delays Behavioral Fever in Fish.
; ; et al
in Cell Host & Microbe (2017), 21(2), 244-253
Both endotherms and ectotherms (e.g., fish) increase their body temperature to limit pathogen infection. Ectotherms do so by moving to warmer places, hence the term "behavioral fever." We studied the ... [more ▼]
Both endotherms and ectotherms (e.g., fish) increase their body temperature to limit pathogen infection. Ectotherms do so by moving to warmer places, hence the term "behavioral fever." We studied the manifestation of behavioral fever in the common carp infected by cyprinid herpesvirus 3, a native carp pathogen. Carp maintained at 24 degrees C died from the infection, whereas those housed in multi-chamber tanks encompassing a 24 degrees C-32 degrees C gradient migrated transiently to the warmest compartment and survived as a consequence. Behavioral fever manifested only at advanced stages of infection. Consistent with this, expression of CyHV-3 ORF12, encoding a soluble decoy receptor for TNF-alpha, delayed the manifestation of behavioral fever and promoted CyHV-3 replication in the context of a temperature gradient. Injection of anti-TNF-alpha neutralizing antibodies suppressed behavioral fever, and decreased fish survival in response to infection. This study provides a unique example of how viruses have evolved to alter host behavior to increase fitness. [less ▲]Detailed reference viewed: 29 (3 ULg)
Behavioral fever in ectothermic vertebrates.
; ; Vanderplasschen, Alain
in Developmental & Comparative Immunology (2017), 66
Fever is an evolutionary conserved defense mechanism which is present in both endothermic and ectothermic vertebrates. Ectotherms in response to infection can increase their body temperature by moving to ... [more ▼]
Fever is an evolutionary conserved defense mechanism which is present in both endothermic and ectothermic vertebrates. Ectotherms in response to infection can increase their body temperature by moving to warmer places. This process is known as behavioral fever. In this review, we summarize the current knowledge on the mechanisms of induction of fever in mammals. We further discuss the evolutionary conserved mechanisms existing between fever of mammals and behavioral fever of ectothermic vertebrates. Finally, the experimental evidences supporting an adaptive value of behavioral fever expressed by ectothermic vertebrates are summarized. [less ▲]Detailed reference viewed: 36 (3 ULg)
Viral glycoprotein gp150 promotes sexual transmission of Murid Herpesvirus-4
Zeippen, Caroline ; Javaux, Justine ; Xiao, Xue et al
Poster (2016, November 28)
Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of ... [more ▼]
Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of immune response. Understanding these strategies is likely to be important to control infection. Interestingly, we recently observed that Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus infecting laboratory mice, could be sexually transmitted between mice. This model offers therefore the opportunity to understand the mechanisms underlying natural transmission. Some of these mechanisms could rely on the glycoprotein 150 (gp150), which could limit virus neutralization and promote the release of infectious particles from cells. In this study, we tested therefore the importance of gp150 in the context of MuHV-4 sexual transmission. Briefly, female mice were infected with WT or gp150- strains expressing luciferase. They were imaged with an in vivo imaging system to follow infection. When lytic replication was observed in the genital tract, infected females were mated with naïve males to compare the capacity of transmission of the two strains. Our results show that, while the gp150- strain has no deficit in reaching and replicating in the female genital tract, it displays a major deficit of sexual transmission in comparison with WT virions. Interestingly, this deficit appears to reflect a deficit of virions release from vaginal epithelial cells. Altogether, our results show that, while gp150 is not required for efficient dissemination and maintenance of MuHV-4 within its host, it is essential for efficient transmission, by promoting the releasing of infectious particles from the mucosal cells. [less ▲]Detailed reference viewed: 34 (3 ULg)
Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever.
; ; Dewals, Benjamin G et al
in mSphere (2016), 1(4), 00108-16
Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has ... [more ▼]
Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has been hindered by the absence of methods to attenuate or modify the virus, since it cannot be propagated in vitro. As an alternative for vaccine development, in this study, we tested the hypothesis that one of the SA-MCF vaccine candidate targets, OvHV-2 glycoprotein B (gB), could be expressed by a nonpathogenic alcelaphine herpesvirus 1 (AlHV-1) and then evaluated the potential of the AlHV-1/OvHV-2 chimera to be used as a vaccine and a diagnostic tool. The construction and characterization of an AlHV-1/OvHV-2 chimeric virus that is nonpathogenic and expresses an OvHV-2 vaccine target are significant steps toward the development of an SA-MCF vaccine and also provide a valuable means to study OvHV-2 biology. [less ▲]Detailed reference viewed: 29 (4 ULg)
Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1.
Myster, Françoise ; ; et al
in Scientific Reports (2016), 6
Alcelaphine herpesvirus 1 (AlHV-1) is a gammaherpesvirus carried asymptomatically by wildebeest. Upon cross-species transmission, AlHV-1 induces malignant catarrhal fever (MCF), a fatal ... [more ▼]
Alcelaphine herpesvirus 1 (AlHV-1) is a gammaherpesvirus carried asymptomatically by wildebeest. Upon cross-species transmission, AlHV-1 induces malignant catarrhal fever (MCF), a fatal lymphoproliferative disease of ruminants, including cattle. The strain C500 has been cloned as an infectious, pathogenic bacterial artificial chromosome (BAC) that is used to study MCF. Although AlHV-1 infection can be established in cell culture, multiple passages in vitro cause a loss of virulence associated with rearrangements of the viral genome. Here, sequencing of the BAC clone showed that the long unique region (LUR) of the genome is nearly identical to that of the previously sequenced strain from which the BAC was derived, and identified the duplication and translocation of a region from within LUR, containing the entire coding sequences of ORF50-encoding reactivation transactivator Rta and A6-encoding bZIP protein genes. The duplicated region was further located to a position within the terminal repeat (TR) and its deletion resulted in lower ORF50 expression levels and reduced viral fitness. Finally, the presence of a similar but not identical duplication and translocation containing both genes was found in AlHV-1 strain WC11. These results indicate that selection pressure for enhanced viral fitness may drive the duplication of ORF50 and A6 in AlHV-1. [less ▲]Detailed reference viewed: 17 (2 ULg)
Long term-cultured and cryopreserved primordial germ cells from various chicken breeds retain high proliferative potential and gonadal colonisation competency
Tonus, Céline ; ; Ectors, Fabien et al
in Reproduction, Fertility and Development (2016), 28(5), 628-639Detailed reference viewed: 151 (75 ULg)
Deletion of Murid Herpesvirus 4 ORF63 Affects the Trafficking of Incoming Capsids toward the Nucleus.
Latif, Muhammad Bilal ; Machiels, Bénédicte ; Xiao, Xue et al
in Journal of virology (2016), 90(5), 2455-72
Gammaherpesviruses are important human and animal pathogens. Despite the fact that they display the classical architecture of herpesviruses, the function of most of their structural proteins is still ... [more ▼]
Gammaherpesviruses are important human and animal pathogens. Despite the fact that they display the classical architecture of herpesviruses, the function of most of their structural proteins is still poorly defined. This is especially true for tegument proteins. Interestingly, a potential role in immune evasion has recently been proposed for the tegument protein encoded by Kaposi's sarcoma-associated herpesvirus open reading frame 63 (ORF63). To gain insight about the roles of ORF63 in the life cycle of a gammaherpesvirus, we generated null mutations in the ORF63 gene of murid herpesvirus 4 (MuHV-4). We showed that disruption of ORF63 was associated with a severe MuHV-4 growth deficit both in vitro and in vivo. The latter deficit was mainly associated with a defect of replication in the lung but did not affect the establishment of latency in the spleen. From a functional point of view, inhibition of caspase-1 or the inflammasome did not restore the growth of the ORF63-deficient mutant, suggesting that the observed deficit was not associated with the immune evasion mechanism identified previously. Moreover, this growth deficit was also not associated with a defect in virion egress from the infected cells. In contrast, it appeared that MuHV-4 ORF63-deficient mutants failed to address most of their capsids to the nucleus during entry into the host cell, suggesting that ORF63 plays a role in capsid movement. In the future, ORF63 could therefore be considered a target to block gammaherpesvirus infection at a very early stage of the infection. IMPORTANCE: The important diseases caused by gammaherpesviruses in human and animal populations justify a better understanding of their life cycle. In particular, the role of most of their tegument proteins is still largely unknown. In this study, we used murid herpesvirus 4, a gammaherpesvirus infecting mice, to decipher the role of the protein encoded by the viral ORF63 gene. We showed that the absence of this protein is associated with a severe growth deficit both in vitro and in vivo that was mainly due to impaired migration of viral capsids toward the nucleus during entry. Together, our results provide new insights about the life cycle of gammaherpesviruses and could allow the development of new antiviral strategies aimed at blocking gammaherpesvirus infection at the very early stages. [less ▲]Detailed reference viewed: 27 (6 ULg)
Structural Proteomics of Herpesviruses.
; Gillet, Laurent ; Vanderplasschen, Alain et al
in Viruses (2016), 8(2),
Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have ... [more ▼]
Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefore, a better understanding of their complex lifecycle is needed. In particular, while the genome of numerous herpesviruses has been sequenced, the exact composition of virions remains unknown for most of them. Mass spectrometry has recently emerged as a central method and has permitted fundamental discoveries in virology. Here, we review mass spectrometry-based approaches that have recently allowed a better understanding of the composition of the herpesvirus virion. In particular, we describe strategies commonly used for proper sample preparation and fractionation to allow protein localization inside the particle but also to avoid contamination by nonstructural proteins. A collection of other important data regarding post-translational modifications or the relative abundance of structural proteins is also described. This review also discusses the poorly studied importance of host proteins in herpesvirus structural proteins and the necessity to develop a quantitative workflow to better understand the dynamics of the structural proteome. In the future, we hope that this collaborative effort will assist in the development of new strategies to fight these infections. [less ▲]Detailed reference viewed: 38 (6 ULg)
Bovine Herpesvirus 4 Modulates Its beta-1,6-N-Acetylglucosaminyltransferase Activity through Alternative Splicing.
Lété, Céline ; ; Machiels, Bénédicte et al
in Journal of virology (2016), 90(4), 2039-51
Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their ... [more ▼]
Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their profit different pathways of glycan synthesis. Thus, the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. Surprisingly, we show in this study that, as opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. Antibodies generated against the Bo17 C terminus showed that the two forms of Bo17 are expressed in BoHV-4 infected cells, but enzymatic assays revealed that the spliced form is not active. In order to reveal the function of these two forms, we then generated recombinant strains expressing only the long or the short form of Bo17. Although we did not highlight replication differences between these strains, glycomic analyses and lectin neutralization assays confirmed that the splicing of the Bo17 gene gives the potential to BoHV-4 to fine-tune the global level of core 2 branching activity in the infected cell. Altogether, these results suggest the existence of new mechanisms to regulate the activity of glycosyltransferases from the Golgi apparatus. IMPORTANCE: Viruses are masters of adaptation that hijack cellular pathways to allow their growth. Glycans play a central role in many biological processes, and several studies have highlighted mechanisms by which viruses can affect glycosylation. Glycan synthesis is a nontemplate process regulated by the availability of key glycosyltransferases. Interestingly, bovine herpesvirus 4 encodes one such enzyme which is a key enzyme for the synthesis of complex O-glycans. In this study, we show that, in contrast to cellular homologues, this virus has evolved to alternatively express two proteins from this gene. While the first one is enzymatically active, the second results from the alternative splicing of the region encoding the catalytic site of the enzyme. We postulate that this regulatory mechanism could allow the virus to modulate the synthesis of some particular glycans for function at the location and/or the moment of infection. [less ▲]Detailed reference viewed: 34 (8 ULg)
Small RNA deep sequencing identifies viral microRNAs during malignant catarrhal fever induced by alcelaphine herpesvirus 1
Sorel, Océane ; ; Myster, Françoise et al
in Journal of General Virology (The) (2015), 96(11), 3360-3372
Alcelaphine herpesvirus 1 (AlHV-1) is a c-herpesvirus (c-HV) carried asymptomatically by wildebeest. Upon cross-species transmission, AlHV-1 induces a fatal lymphoproliferative disease named malignant ... [more ▼]
Alcelaphine herpesvirus 1 (AlHV-1) is a c-herpesvirus (c-HV) carried asymptomatically by wildebeest. Upon cross-species transmission, AlHV-1 induces a fatal lymphoproliferative disease named malignant catarrhal fever (MCF) in many ruminants, including cattle, and the rabbit model. Latency has been shown to be essential for MCF induction. However, the mechanisms causing the activation and proliferation of infected CD8+T cells are unknown. Many c-HVs express microRNAs (miRNAs). These small non-coding RNAs can regulate expression of host or viral target genes involved in various pathways and are thought to facilitate viral infection and/or mediate activation and proliferation of infected lymphocytes. The AlHV-1 genome has been predicted to encode a large number of miRNAs. However, their precise contribution in viral infection and pathogenesis in vivo remains unknown. Here, using cloning and sequencing of small RNAs we identified 36 potential miRNAs expressed in a lymphoblastoid cell line propagated from a calf infected with AlHV-1 and developing MCF. Among the sequenced candidate miRNAs, 32 were expressed on the reverse strand of the genome in two main clusters. The expression of these 32 viral miRNAs was further validated using Northern blot and quantitative reverse transcription PCR in lymphoid organs of MCF- developing calves or rabbits. To determine the concerted contribution in MCF of 28 viral miRNAs clustered in the non-protein-coding region of the AlHV-1 genome, a recombinant virus was produced. The absence of these 28 miRNAs did not affect viral growth in vitro or MCF induction in rabbits, indicating that the AlHV-1 miRNAs clustered in this non-protein-coding genomic region are dispensable for MCF induction. [less ▲]Detailed reference viewed: 38 (4 ULg)
The genome of a tortoise herpesvirus (testudinid herpesvirus 3) has a novel structure and contains a large region that is not required for replication in vitro or virulence in vivo.
Gandar, Frederic ; ; et al
in Journal of Virology (2015), 89(22), 11438-11456
Testudinid herpesvirus 3 (TeHV-3) is the causative agent of a lethal disease affecting several tortoise species. The threat that this virus poses to endangered animals is focusing efforts on ... [more ▼]
Testudinid herpesvirus 3 (TeHV-3) is the causative agent of a lethal disease affecting several tortoise species. The threat that this virus poses to endangered animals is focusing efforts on characterizing its properties, in order to enable the development of prophylactic methods. We have sequenced the genomes of the two most studied TeHV-3 strains (1976 and 4295). TeHV-3 strain 1976 has a novel genome structure and is most closely related to a turtle herpesvirus, thus supporting its classification into genus Scutavirus, subfamily Alphaherpesvirinae, family Herpesviridae. The sequence of strain 1976 also revealed viral counterparts of cellular interleukin-10 and semaphorin, which have not been described previously in members of subfamily Alphaherpesvirinae. TeHV-3 strain 4295 is a mixture of three forms (m1, m2, and M), in which, in comparison to strain 1976, the genomes exhibit large, partially overlapping deletions of 12.5 to 22.4 kb. Viral subclones representing these forms were isolated by limiting dilution assays, and each replicated in cell culture comparably to strain 1976. With the goal of testing the potential of the three forms as attenuated vaccine candidates, strain 4295 was inoculated intranasally into Hermann's tortoises (Testudo hermanni). All inoculated subjects died, and PCR analyses demonstrated the ability of the m2 and M forms to spread and invade the brain. In contrast, the m1 form was detected in none of the organs tested, suggesting its potential as the basis of an attenuated vaccine candidate. Our findings represent a major step toward characterizing TeHV-3 and developing prophylactic methods against it. [less ▲]Detailed reference viewed: 65 (15 ULg)
Long term culture, cryopreservation and genetic modification of chicken primordial germ cells
Tonus, Céline ; Garcia Gil, Francisco José ; et al
Poster (2015, October 16)
Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties ... [more ▼]
Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties in vitro and are foreseen as promising tools for developing efficient avian genetic engineering and preservation of germplasm. We propose original methods that allow long term expansion, efficient cryopreservation and genetic modification of primary cultures of undifferentiated PGCs. PGCs are collected from embryonic blood during their migratory period and grown in cell-culture insert in the presence of feeder cells (BRL). This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of PGCs lines. Forty percent of blood samples gave rise to lines originating from three commercial layer and two Belgian endangered breeds. PGCs lines were characterized for the expression of the stem cells and PGCs marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. All lines were male although isolated from pooled male and female blood samples. Two cryopreservation methods were developed based upon slow-freezing and aseptic vitrification. Both have shown a similar effectiveness in allowing storage without phenotype drift. Stably expressing lines were obtained by Lipofectamine® mediated transfection of a GFP plasmid. PGCs were subsequently injected in recipient embryos. Persistence of exogenous PGCs in the developing gonad of recipient embryos confirmed that PGCs retain their gonadal colonisation ability, both after long-term culture and after cryopreservation. [less ▲]Detailed reference viewed: 128 (14 ULg)
Helminth-induced inflammation controls murine γ-herpesvirus replication in the lung
Dougall, Annette ; Rolot, Marion ; Vanderplasschen, Alain et al
Conference (2015, September)Detailed reference viewed: 55 (5 ULg)
The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation
; ; et al
in Vaccine (2015), 33Detailed reference viewed: 29 (2 ULg)
Rational Development of an Attenuated Recombinant Cyprinid Herpesvirus 3 Vaccine Using Prokaryotic Mutagenesis and In Vivo Bioluminescent Imaging
Boutier, Maxime ; Ronsmans, Maygane ; et al
in PLoS Pathogens (2015), 11(2), 1004690
Cyprinid herpesvirus 3 (CyHV 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We ... [more ▼]
Cyprinid herpesvirus 3 (CyHV 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We produced single deleted recombinants using prokaryotic mutagenesis. When producing a recombinant lacking open reading frame 134 (ORF134), we unexpectedly obtained a clone with additional deletion of ORF56 and ORF57. This triple deleted recombinant replicated efficiently in vitro and expressed an in vivo safety/efficacy profile compatible with use as an attenuated vaccine. To determine the role of the double ORF56-57 deletion in the phenotype and to improve further the quality of the vaccine candidate, a series of deleted recombinants was produced and tested in vivo. These experiments led to the selection of a double deleted recombinant lacking ORF56 and ORF57 as a vaccine candidate. The safety and efficacy of this strain were studied using an in vivo bioluminescent imaging system (IVIS), qPCR, and histopathological examination, which demonstrated that it enters fish via skin infection similar to the wild type strain. However, compared to the parental wild type strain, the vaccine candidate replicated at lower levels and spread less efficiently to secondary sites of infection. Transmission experiments allowing water contamination with or without additional physical contact between fish demonstrated that the vaccine candidate has a reduced ability to spread from vaccinated fish to naïve sentinel cohabitants. Finally, IVIS analyses demonstrated that the vaccine candidate induces a protective mucosal immune response at the portal of entry. Thus, the present study is the first to report the rational development of a recombinant attenuated vaccine against CyHV 3 for mass vaccination of carp. We also demonstrated the relevance of the CyHV 3 carp model for studying alloherpesvirus transmission and mucosal immunity in teleost skin. [less ▲]Detailed reference viewed: 67 (12 ULg)
Cyprinid Herpesvirus 3 Il10 Inhibits Inflammatory Activities of Carp Macrophages and Promotes Proliferation of Igm+ B Cells and Memory T Cells in a Manner Similar to Carp Il10.
; ; et al
in Journal of immunology (Baltimore, Md. : 1950) (2015), 195(8), 3694-704
Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease of carp and encodes for an Il10 homolog (ORF134). Our previous studies with a recombinant ORF134-deleted strain and the derived ... [more ▼]
Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease of carp and encodes for an Il10 homolog (ORF134). Our previous studies with a recombinant ORF134-deleted strain and the derived revertant strain suggested that cyprinid herpesvirus 3 Il10 (CyHV-3 Il10 [cyhv3Il10]) is not essential for viral replication in vitro, or virulence in vivo. In apparent contrast, cyhv3Il10 is one of the most abundant proteins of the CyHV-3 secretome and is structurally very similar to carp Il10 and also human IL10. To date, studies addressing the biological activity of cyhv3Il10 on cells of its natural host have not been performed. To address the apparent contradiction between the presence of a structurally conserved Il10 homolog in the genome of CyHV-3 and the lack of a clear phenotype in vivo using recombinant cyhv3Il10-deleted viruses, we used an in vitro approach to investigate in detail whether cyhv3Il10 exerts any biological activity on carp cells. In this study, we provide direct evidence that cyhv3Il10 is biologically active and, similarly to carp Il10, signals via a conserved Stat3 pathway modulating immune cells of its natural host, carp. In vitro, cyhv3Il10 deactivates phagocytes with a prominent effect on macrophages, while also promoting proliferation of Igm(+) B cells and memory T cells. Collectively, this study demonstrates a clear biological activity of cyhv3Il10 on cells of its natural host and indicates that cyhv3Il10 is a true viral ortholog of carp Il10. Furthermore, to our knowledge, this is the first report on biological activities of a nonmammalian viral Il10 homolog. [less ▲]Detailed reference viewed: 18 (1 ULg)
The Structure of the Cyprinid herpesvirus 3 ORF112-Zalpha.Z-DNA Complex Reveals a Mechanism of Nucleic Acids Recognition Conserved with E3L, a Poxvirus Inhibitor of Interferon Response.
; ; Boutier, Maxime et al
in Journal of Biological Chemistry (2015), 290(52), 30713-25
In vertebrate species, the innate immune system down-regulates protein translation in response to viral infection through the action of the double-stranded RNA (dsRNA)-activated protein kinase (PKR). In ... [more ▼]
In vertebrate species, the innate immune system down-regulates protein translation in response to viral infection through the action of the double-stranded RNA (dsRNA)-activated protein kinase (PKR). In some teleost species another protein kinase, Z-DNA-dependent protein kinase (PKZ), plays a similar role but instead of dsRNA binding domains, PKZ has Zalpha domains. These domains recognize the left-handed conformer of dsDNA and dsRNA known as Z-DNA/Z-RNA. Cyprinid herpesvirus 3 infects common and koi carp, which have PKZ, and encodes the ORF112 protein that itself bears a Zalpha domain, a putative competitive inhibitor of PKZ. Here we present the crystal structure of ORF112-Zalpha in complex with an 18-bp CpG DNA repeat, at 1.5 A. We demonstrate that the bound DNA is in the left-handed conformation and identify key interactions for the specificity of ORF112. Localization of ORF112 protein in stress granules induced in Cyprinid herpesvirus 3-infected fish cells suggests a functional behavior similar to that of Zalpha domains of the interferon-regulated, nucleic acid surveillance proteins ADAR1 and DAI. [less ▲]Detailed reference viewed: 19 (0 ULg)
Cyprinid herpesvirus 3 : an archetype of fish alloherpesviruses
Boutier, Maxime ; Ronsmans, Maygane ; Rakus, Krzysztof et al
in Advances in Virus Research (2015), 93Detailed reference viewed: 72 (13 ULg)
The alpha2,3-Sialyltransferase Encoded by Myxoma Virus Is a Virulence Factor that Contributes to Immunosuppression.
; ; et al
in PloS one (2015), 10(2), 0118806
Myxoma virus (MYXV) induces a lethal disease called Myxomatosis in European rabbits. MYXV is one of the rare viruses that encodes an alpha2,3-sialyltransferase through its M138L gene. In this study, we ... [more ▼]
Myxoma virus (MYXV) induces a lethal disease called Myxomatosis in European rabbits. MYXV is one of the rare viruses that encodes an alpha2,3-sialyltransferase through its M138L gene. In this study, we showed that although the absence of the enzyme was not associated with any in vitro deficit, the M138L deficient strains are highly attenuated in vivo. Indeed, while all rabbits infected with the parental and the revertant strains died within 9 days post-infection from severe myxomatosis, all but one rabbit inoculated with the M138L deficient strains survived the infection. In primary lesions, this resistance to the infection was associated with an increased ability of innate immune cells, mostly neutrophils, to migrate to the site of virus replication at 4 days post-infection. This was followed by the development of a better specific immune response against MYXV. Indeed, at day 9 post-infection, we observed an important proliferation of lymphocytes and an intense congestion of blood vessels in lymph nodes after M138L knockouts infection. Accordingly, in these rabbits, we observed an intense mononuclear cell infiltration throughout the dermis in primary lesions and higher titers of neutralizing antibodies. Finally, this adaptive immune response provided protection to these surviving rabbits against a challenge with the MYXV WT strain. Altogether, these results show that expression of the M138L gene contributes directly or indirectly to immune evasion by MYXV. In the future, these results could help us to better understand the pathogenesis of myxomatosis but also the importance of glycans in regulation of immune responses. [less ▲]Detailed reference viewed: 44 (12 ULg)


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subset: commoncrawl


text:
|4,305 sq. ft.
|Property type
|Minimum Stay
|3 - 7 nights
Villa CAROLA is bright and extremely spacious. This property has unique views, many balconies and terraces and a wonderful heatable big pool. Villa CAROLA is ideal for friends sharing or family gatherings. We also cater for small infants (2 high-chairs and 2 baby-beds). The villa is fully equipped. There is air conditioning throughout. We also have strong wireless internet.
The pool is heatable!
Renovation works: January - 18 April 2014
3 beautiful new contemporary en suite bathrooms
3 new build-in wardrobes
new carpets
Major renovations took also place during spring 2012:
New master bathroom (bath + big shower), new master WC area, 2 new dressing rooms, a new shower room...
Ideal for 8 - 12people
Parking for up to 6 cars.
Comments from clients: We, a bunch of friends stayed at villa CAROLA last year during May for 2 weeks. We had the most wonderful time, just relaxing and sightseeing. Bettina gave us a wonderful welcome and great tips for places to see. We can highly recommend the villages of Eze (above Monaco) and Gordon. We will definitely be back and will recommend villa CAROLA to our families and friends. Gretel, Micha, Katrin, Annegret, Stefan and Juliana from Wien, Austria
Comments from clients: Simply wonderful! We were absolutely speechless when we arrived. The view is gorgeous! 1 week was definitely to short since there is so much to see! St. Paul is only 10 minutes away on foot what a beautiful village and the sea is in very easy reach. Bettina thank you for your suggestion to take the children for a day out at the Marineland in Biot. They were absolutely thrilled and still talk about the shark tunnel and the dolphin shows.
We definitely plan to come back next year. Family Kehricht from Frankfurt, Germany
Comments from clients: Was great! Thank you Bettina! The villa is 1 A, super big. We had a smashing time. The pool was great and weather perfect. We'll book 2 weeks next time. The Antibes food market was a dream. Highly recommended!!! Best regards Ralf, Heiko, Martin, Dieter, Eddy, Franco, Volker, Tobias, Mark, Klaus, Stefan, Matthias and Peter from Switzerland, Graubünden
My name is Bettina. I am the key holder. I will meet and greet you on arrival and assist you during your stay at villa CAROLA.
I speak English, German and French fluently.
This outstanding property is very comfortable and spacious with most amazing breathtaking views.
The villa is ideally located between Nice and Antibes and in walking distance to the famous village of St. Paul de Vence.
Major cities such as Nice, Cannes and Monaco can easily be reached by car. Driving time: 20 to 45min.
The location is truly ideal.
The swimming pool can be heated during mid-season.
Cost: 300.-E per week
Nous sommes du Nord, alors St. Paul..., quelle différence! Toutes ces couleurs et toutes ces saveurs! La beauté du paysage, la proximité de la mer et ces pitoresques villages dans les allentours! C'est d'une bauté!!! Nous nous sommes beaucoup promenés mais aussi bien reposés dans cette magnifique villa! La vue est incroyable. Merci pour votre assistance et bons conseilles. Cordialement, famille Dupond, St. Quentin
We had a perfect stay at Villa Carola. 3 families with teen kids loved the pool, terreses and evenenings at the BBQ grill. Walking distance to St. Pauls. Higly recommendable.
Först och främst måste vi framhålla våran värd Bettina, mycket proffesionell och hjälpsam.
Villa Carola var ett super mysigt ställe där jag med vänner spenderade 8 dagar, sammanlagt 11 vuxna och 2 barn. Gott om plats, jätte fina rum och riktigt fräscha badrum och toaletter! Utsikten mot den gamla staden st Paul de vence och havet var helt fantastisk. Poolen var mycket uppskattad av hela sällskapet och grill platsen användes flitigt!
Riktigt kul att det fanns ett pingis bord i garage delen.
Den gamla staden (st Paul de vence) var jätte mysig, fanns bra restauranger å mycket att se, kan rekommendera vin provning.
Närheten av Nice, Cannes och Monaco gjorde det lätt att göra dags utflykter.
Men gillar man att ha det lugnt och umgås med nära och kära är villa Carola ett perfekt ställe att koppla av på och bara vara.
Vi kommer absolut försöka att komma tillbaka så snart vi kan.
Två tummar upp 👍🏽👍🏽
/Mattias och Elina
Wir haben im Juni 2016 einen großartigen Aufenthalt mit vier Pärchen in Villa Carola verbracht. Das Haus und der Außenbereich sind sehr großzügig, so dass immer genug Platz vorhanden war. Für uns war es immer eine harte Entscheidung einfach nur im Garten am beheizten Pool bei einem BBQ (Grill vorhanden) und einer Runde Boule auf der hauseigenen Anlage zu entspannen oder etwas von der interessanten Region zu entdecken. Das Haus ist komplett ausgestattet: Küchenutensilien, Waschmaschine, Wifi, TV, Bouleset... uns fehlte nichts.
Die Abwicklung und der Service waren unkompliziert und sehr nett. Von der Buchung bis zur Abreise wurden Fragen zügig beantwortet und Tipps gegeben.
Wir kommen definitiv wieder. Vielen lieben Dank nochmal.
We stayed at Villa Carola in mid-May for a week. There are amazing views from the villa to St Paul de Vence and down to the Mediterranean in the distance. The driveway to the villa is very steep, and there are no footpaths once at the road, so essential to have a car for day trips, supermarket trips etc. Comprising a large master suite on the lower ground floor, three double bedrooms on the first floor (all en suite), a huge living room, reception hall, kitchen and utility room on the ground floor, it accommodated our family of six adults and a baby perfectly. The swimming pool was fantastic, and we were lucky enough to have lovely sunny weather for our week there so were not tempted to leave the villa that often (other than trips to the Leclerc supermarket a few miles away). The property was immaculate and very well appointed, especially the bathrooms. The kitchen, whilst big, could do with updating. The bbq could also do with an upgrade. Bettina, the property manager, was extremely nice and very helpful. We would have no hesitation in recommending Villa Carola - we had a wonderful week's holiday there!
We spent a fabulous week at this very spacious villa. The views of St Paul de Vence and the sea around the Cap d'Antibes from the pool, main terrace & upstairs bedrooms are absolutely stunning.
We enjoyed an evening stroll into St Paul de Vence before supper & a drink in the Cafe de la Place, watching the locals playing boules.
Memorable evening meals with 12 family & friends around the large table on the covered terrace outside the kitchen, laughing together, sampling the local rose & with the lighthouse on the cap d'Antibes flashing on the horizon.
We will definitely return.
Stunning location and views.comfortable.
Modern styled bathrooms.
Well equipped.
Shame keyboard was not connected.
Kitchen could do with updating
St. Paul is a very charming medieval village and known for its priceless art: paintings, sculptures and glassware. It also has very good restaurants, the most famous one being 'La Colombe d'Or'. The views around St. Paul are heavenly, green hills, loads of very colourful flowers in spring, many orange and lemon trees and most importantly a grand view onto the Mediterranean sea.
St. Paul has a privileged location between Nice and Antibes. Only 10min to the sea, 15min to the airport and around 45min to Monaco and Cannes. Many attractive villages like: Eze, Vence, Tourettes, Gourdon and Mougin are within 30 to 60min driving distance.
Maximum of 12 people
Car is a must!
Golf: There are several golf areas within 20 to 50min drive:
Rouret, Grasse, Valbonne etc.
Ski: nearest skiing resorts:
Gréolière les Neiges, Isola 2000 and Auron
Reliable and cheap taxi company from Nice airport to villa:
|Rate Period
|Weekend Night
high season
Jul 15 2017 - Aug 21 2017
7 night minimum stay
mid-high season
Aug 22 2017 - Sep 1 2017
4 night minimum stay
mid season
Sep 2 2017 - Sep 15 2017
3 night minimum stay
mid season
Sep 16 2017 - Nov 3 2017
3 night minimum stay
low season
Nov 4 2017 - Dec 22 2017
3 night minimum stay
Dec 23 2017 - Dec 29 2017
7 night minimum stay
Flat fee
New Year
Dec 30 2017 - Jan 2 2018
4 night minimum stay
Flat fee
My Standard Rate
3 night minimum stay
|No additional mandatory fees
cash on arrival:
350.-E for reception, laundry & end of stay cleaning, this is up to 8 people
Any extra person: 20.-E extra (once)
500.-E security deposit
this will be returned at the end of your stay if all ok
2 baby-beds available as well as 2 high chairs
300.-E per week for pool heating (1 - 7 days, same rate!)
This is on request! Pool heating needed during months: April, May, June, September and October
No pool heating available: November to March!


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The nose of this Santa Rita-originated Pinot Noir offers tantalizing black cherry and raspberry notes, plus hints of black pepper, but the body is heavily extracted to the point where it starts to get a bit pruny. Notes of green olive, currant jelly, and brewed tea. Fun at the start, it ultimately takes things too far out of bounds.
B / $44 / sanfordwinery.com
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The Final Verdict on Value {A Video}
Here it is, the final post in our Verdict on Value series. {Thank you to Arabah Joy for gathering our group for this series. We've prayed for you dear reader, that you have been encouraged and set free!} Haven't we had a rich time over the past six weeks with fellow sisters and the Word … [Read more…]


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FIU Grad's Photo Series Aims to Change Perception of Islam
Sana Ullah and her sister were shopping at Sawgrass Mills when they realized it was time to pray. They grabbed "probably the ugliest shirt" in the store, she recalls, and hurried into a changing room.
The whole situation had Ullah laughing to herself. It was hardly the first time she'd prayed somewhere unusual: As a devout Muslim, she stops what she's doing five times a day to pray. This time, though, it gave the South Florida native an idea.
"I can name so many locations I've prayed before, and maybe most of the places I've prayed have not been inside a mosque," the 24-year-old photographer and Florida International University graduate says. "So I decided it'd be really fun to do a series where I could take pictures of people praying in locations outside of mosques."
Places You'll Pray started in January 2015 as a fun project but began taking on more weight in recent months, as anti-Muslim incidents spiked (a report by Georgetown University's Bridge Initiative found 2015 saw the most of any year since the 9/11 attacks). Republican presidential nominee Donald Trump advocated banning Muslims from entering the United States following the Islamic State-inspired mass shooting in San Bernardino.
Now, Ullah hopes her photo series — which shows people praying at sites including the Grand Canyon, Universal Studios Orlando, and Central Park, as well at milestones such as high-school prom, a graduation ceremony, and a wedding — will help change how Muslims are viewed. She has posted more than 300 of the images on Instagram. Some are taken by her; others have been submitted by people around the world. Maybe people will look at them and see that peace is central to Islam, she says.
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"If one person can see Muslims in a brighter light or in a positive way," Ullah says, "I think I've succeeded."
Lately, Ullah has noticed that people have become "a little more bold" in their prejudices, she says, adding, "I never noticed how many friends I have on Facebook that are not big fans of Muslims." She's also gotten comments from people on the streets — usually along the lines of "go back to your own country."
If the person doesn't seem threatening, Ullah will respond that this is her country. ("You can tell I'm a Miamian because I always have something to say back," she quips.)
Ullah says many do not understand the Islamic faith. She wants Places You'll Pray to encourage people to ask questions. "I feel like if we keep ideas in our head and don't ask the questions in our heads, we start to answer them ourselves," she says. "And lots of times, it's wrong."
Because of recent events, Ullah says, she believes Muslims are not praying in public as often. She adds that some people have told her she's brave for wearing hijab, and some of her friends don't want anyone to know they're Muslim. So her project, which is also part of her thesis for graduate school at George Washington University, has another goal.
"It's kind of like, 'Hear us now; look at us. Whatever you feel against Muslims, it's not affecting us, and we're still going to practice, we're still going to do what we do,'" Ullah says. "I guess to encourage people it's OK. It's OK to be Muslim."
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Christmas Elf Figures and Decorations
For centuries, Northern Europeans believed their homes were protected by mischievous gnomes. When the Santa Claus tradition made it to Scandinavia in the 1800's, writers quickly adapted the gnomes into Santa's friends and helpers. Elf figurines in porcelain, glass, plastic and rubber are now common sights in antique shops; not to mention elf images on plates, ornaments, sweaters, and other media.
Best of the Web ("Hall of Fame")
Papa Teds Place
The late Theodore H. Althof, Jr's beautiful collection of vintage dimestore cardboard Christmas 'putz' village hous… [read review or visit site]
Bohemian Christmas Decorations
This simple site showcases over 500 artistic Gablonz glass bead antique and vintage Christmas tree ornaments, made … [read review or visit site]
Old Soviet Christmas Cards
This site showcases over 1000 old Soviet Christmas and New Years cards from 1950 to 1990. Browse through page after… [read review or visit site]


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22 comments found.
Hello. The wordpress version is no longer available at the address: http://themeforest.net/item/argo-modern-onepage-metro-ui-wordpress-theme/4589714
Can you please help how we can purchase the wordpress version?
Hi, so sorry when the Argo WordPress version is not available for sale, now.
Hi Dzoan , i buyed a wp version, and i just need to know where i found the icons images?


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
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What order form is best to use?Asked by dasilvastudio on March 04, 2016 at 07:15 PM
I sell different photo packages. I need an order form where I can offer multiple packages and have conditions set up for each package to offer more options if they select certain packages. Do you have an order form that fits that type?
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We do have several templates that you can modify and use for your order form. You can check them out by following this link: https://www.jotform.com/form-templates/category/order-form
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Setting Up Your First Order Form
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This is a continuation / reedit of my earlier blog trying to suggest a possible link between the Kaiju and Lovecraft's Cthulhu Mythos.
To start, I have never been satisfied with the idea that Godzilla was nothing more that a radioactive (and somehow resurrected) dinosaur. That story never seemed good enough to encompass the mythic quality of what many consider, "The King of all Monsters". In the 2014 version of the film the writers suggest what seems to me to be a much more satisfying origin for Godzilla and the other Kaiju. The film goes over this very quickly and without much detail, but the suggestion is that the Kaiju predate the dinosaurs by perhaps millions of years, that they feed off of radioactivity, and do not "eat" in any sense of the word that we would recognize. The filmmakers go on to suggest that as the primordial earth started to cool, and presumably became less radioactive, Godzilla and the other Kaiju went into a dormant state close to the planet's core.
This explanation gives the Kaiju a much more mythic and grand origin; but it also provides an interesting possible link to Lovecraft's Cthulhu Myhtos. My understanding of Lovecraft's elder gods is that they originated in space, that they once controlled the earth, and that they are now in various stages of suspended animation under the earth, the sea, or back in space. Perhaps the Kaiju could be envisioned as related in some way to these elder gods. They could be presented as having developed shortly after the big bang as interstellar organisms that consumed radioactive energy. Slowly they evolved into conscious beings with unique characteristics. Perhaps their interactions even play some role in the overall balance of the universe between creation and entropy. Some Kaiju, like Godzilla, eventually came to rest on a primordial earth.
This is where the Kaiju would diverge dramatically from the Chulhu Mythos. While Lovecraft's creations were manifestly evil and bent on the extinction of all life on earth, the Kaiju are more varied with some even taking on the role of the defenders of humanity. As for Godzilla, I like the characterization of the creature as aloof and uninterested in the affairs of humans. His concerns clearly center around the other Kaiju and his motivations are probably best left undefined.


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Rares Barbantan
- March 26, 2016
( 183)
This live wallpaper for the Android platform simulates a piece of cloth attached to the back of your mobile device. Using real physics to shake your wallpaper to life, this app also offers some of the most popular patterns found on http://www.colourlovers.com/
Cloth has stopped Cloth has stopped
Could be a lot better Not bad, but the cloth is exactly the size of the screen, so any moment pulls away from the edge. Would prefer it to show the movement without the black space.
Pretty cool Plenty of cloth like backgrounds for you to choose from.
Very pooooorrr Doesn't work
Doesn't work Waste of time.
Won't open on s3 Does not open on my s3 even after after two installs
What's New
added shadows
removed ads


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Get notified when new sales are live
Subscribe to our notifications so you don't miss out on our new sales!
Chuggington Wood Emery Engine
Don't pay more elsewhere for this awesome kids' toy!
Made with real wood | Guaranteed hours of fun!
- Chuggington Wooden Railway toys
- Design: Emery Engine
- Colour: Multi
- Works with other wooden train systems
- Materials: Wood
- Recommended for ages: 3 years+
- Dimensions (approx, cm): 20 x 6.5 x 5 (L x H x D)
- Country of origin: China


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Meanwhile, Arric has been getting ready to give his minions orders, while Wolfgang and everyone at his house talk.
Arric) Okay, did everyone have a good training day?
Persona) Yes!
Christian) Yeah...
Ray) I did, I beat Christian! >=D
Christian) THAT WAS A FLUKE WIN!
Persona) No...Ray actually beat you...
Christian) BUT HIS MOVE DIDN'T WIN IT!
May) So what, you lost...
AcePyrusWolf) WELL YOU LOST TOO!
May) DON'T PUSH IT!
( Arric watches )
( Everyone starts to yell )
May) HOW ABOUT ME AND CHRISTIAN TEAM UP ON YOU TWO TOMORROW, I'M THINKING THAT WILL BE GOOD!
AcePyrusWolf) FINE!
Arric) Hold on...I say who trains with who and this will not happen tomorrow!
( Everyone yells louder )
May) WHAT! WHY!
Arric) What?
May) WHY!
Arric) Hold on...EVERYONE SHUT UP!
( Everyone shuts up )
Arric) Okay...Now what did you say?
May) Why can't we do that for training tomorrow?
Arric) Because...Well maybe you might be able to, we'll see...
May) Okay
Arric) But for now...May, Christian, go to bed...Persona, Ray, Acepyruswolf, stay here...
May) Umm...Okay...
Christian) AWW!
( May pulls Christian, to his room, then goes to her room )
[ 5 minutes later, after Arric knows Christian and May have to be in there rooms for sure, Arric talks ]
Arric) Okay...Persona, Ray, AcePyrusWolf, I'm assigning you to a mission...
Persona) Which is?
Arric) Find Wolfgang, he should be easy to find since everyone knows where he lives...
Ray) AND I WILL MAKE HIM PAY FOR WHAT HE DID TO MY MOM!
Arric) Oh...And I forgot, try to take Samantha here, Wolfgang kidnapped her...
Ray) I WILL!
AcePyrusWolf) ...We'll do this for you, master!
Persona) Should we go right now?
Arric) Thank you and yes...Oh and Persona, you're the leader!
Persona) Really!
Arric) Yeah, since you're my co-leader...
Persona) THANK YOU!
Arric) You're welcome...
Persona) NOW LETS GO!
( Persona, AcePyrusWolf, and Ray teleport )
Team PG vs. Team APW, Episode 7 Part 1/2


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Riders of all abilities can find open play areas offering gentle slopes, as well as challenging hillsides to tempt even the most adventurous of the "high-markers." 544 miles of groomed trails are maintained throughout Valley County making it nearly impossible to take the same route twice in one winter (View Trail Maps). McCall has consistently been in the top ranking for the North Western United States and is currently ranked #6 on the list. Remember to pick up your registration (and mark area 43A) before you head out – proceeds fund continued grooming and maintenance of trails. Check the grooming report and learn where you can purchase registrations at SnowmobileMcCall.net.
Explore two ways to ride: (1) self-guided snowmobile rentals or (2) guided tours. With a snowmobile rental from Cheap Thrills Rentals or CM Backcountry Rentals, create your own adventure! Rentals are delivered to the trailhead and your group can strike out for a ride to Burgdorf Hot Springs, Warren and Secesh, or a trip to one of the many alpine lakes in the area. New to snowmobiling and want to go with a guide? Try a brand new guided snowmobile trip at Brundage Mountain Resort or CM Backcountry Rentals. Their seasoned guides can take you to scenic destinations including Clow Point, Granite Lake, Brundage Reservoir, Hazard Lake, Warren, the Brundage Mountain Lookout, and Burgdorf Hot Springs, among others. Brundage also offers snowmobile rentals for self-guided trips.
Snowmobiling Directory


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Photo: Gina Matsoukas, Running to the Kitchen
Looking for a super-simple summertime supper? Something crisp and refreshing, but a little more substantial than a leafy salad?
Try cooling down with a chilled summery soup.
Make the most of the bounty of fresh fruits and vegetables available at your farmer's market or supermarket (or from your own garden) with these delicious, easy to make recipes:
Cherry Gazpacho
A play on the traditional tomato-based Spanish dish, Megan Kone from Country Cleaver substitutes cherries in her gazpacho for a sweet taste of summer. The cherries add a burst of tartness, she says, which pairs nicely with her garnishes of fresh basil and (you guessed it) more cherries.
Chilled Greek Yogurt Soup
Tori Avey
Talk about dreamy! This soup calls for just eight simple ingredients, including Persian cucumbers and greek yogurt. Founder Tori Avey of the Tori Avey Blog adds milk to her recipe to achieve the true soup texture, and suggests lemon juice or chopped walnuts for even more flavor.
Chilled Strawberry Bisque
Sprinkle Bakes
This sweet strawberry soup from Heather Baird at Sprinkle Bakes makes a wonderful dessert. She even fashions her own caviar with grenadine and Grand Marnier to accompany her chilled bisque. (You probably won't be able to stop at just one bowl, is what we're saying.)
|Fox News Magazine is the official lifestyle magazine of Fox News, covering love, relationships, style, beauty, food, nutrition, fitness, décor and design. Get more tips at Fox News Magazine.


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Movie Reviews and Previews Articles
Movie Reviews and Previews including industry news, new releases, upcoming projects, and box office results
Justin Theroux: on his dog and feminism
Wenn, AKM-GSI
Justin Theroux is currently promoting The Girl On The Train and he was interviewed by ELLE. Everyone who interviews Justin Theroux is going to try to get a quote about Jennifer Aniston Full Story
Garrett Hedlund in New York
FameFlynet, Splash News
I find Garrett Hedlund really attractive. I find his hair really attractive. Check him out in New York yesterday looking really attractive with really attractive hair. The New York Film Festival opens tomorrow. Full Story
Remembering JT's best years
S. Granitz/ Gregg DeGuire/ Steve Sands/ Getty Images
Lifetime is working on an unauthorised Britney Spears movie. It will most definitely be sh-t. But I think I might watch: Justin Timberlake Full Story
Fantastic Beasts has no owls
But it does have a really cool golden bird. A new trailer for Fantastic Beasts and Where to Find Them has come out and this one gives a better look at Newt Scamander's many fantastic beasts, among them, a golden bird that is maybe a thunderbird. There are, however, no owls. Am very disappointed as I got a great grey owl Full Story
What we missed: Jakey's bush
Mike Marsland/ Bruce Glikas/ Getty Images
We missed a lot when World War Brange took over the celebrity news cycle last week. This week's tabloid are about to drop. I suspect we might be getting another round of Brange ugly very soon. Before that happens, let's catch up on one of the most important but overlooked issues from last week: Jake Gyllenhaal Full Story
Justin Theroux on World War Brange
Raymond Hall/ Getty Images
Justin Theroux is currently on promotion for The Girl On The Train. A reporter from Business Insider (via PEOPLE Full Story
Here come Viola & Denzel
Wenn, Jason LaVeris/ Getty Images
The first trailer for Fences was released today, based on the Tony Award and Pulitzer Prize-winning play by August Wilson and directed by Denzel Washington. Both he and Viola Davis Full Story
Molly Shannon and Jesse Plemons in Other People
The "indie cancer dramedy" is a well-established sub-genre populated by films like 50/50, The Fault In Our Stars, Me and Earl and the Dying Girl, and of course the granddaddy of them all, Terms of Endearment. This is such a specific type of film that if you watch the trailer and think it looks like a fake movie trailer from Saturday Night Live, you would be forgiven because this is exactly the sort of movie that would be parodied on SNL. Full Story
Justin Timberlake shares the title
Oh hey. I'm back from my procedure. It was fine at the time. But now I'm home and the freezing has worn off so this is where I find Justin Timberlake in the extended trailer for his concert film, Justin Timberlake + the Tennessee Kids, premiering on Netflix October 12. Full Story
Jennifer Aniston post-Brange
TheStewartofNY/ Splash News
I was talking to a friend of mine this weekend and we played a game called "how long before Brad Pitt gets papped having a friendly lunch with Jennifer Aniston Full Story
Regina Hall and Morris Chestnut in When the Bough Breaks
Andrew Toth/ Paul Archuleta/ JB Lacroix/ Michael Tullberg/ Getty Images
If ever a movie needed to be rated R, it's When the Bough Breaks, the evil-surrogate "thriller" from Screen Gems, the latest in what has become their annual fall tradition of releasing a bad genre movie aimed at black audiences (see also: The Perfect Guy, No Good Deed). I like the idea of a studio deciding that they are going to make the effort every year to put out a movie for an under-served audience, but f*cking hell I wish they'd try a little harder to make a good one. Full Story


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You are here: Home : Topics A-Z : Restructuring
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Porn Star Cited in Ronco CFO Dispute
Why the salary of the CFO in the Veg-o-matic bankruptcy case may hinge on the word of a pornographic video queen. July 27, 2007
Can You Afford to Go Bankrupt?
Claims from those who helped Delta fly out of Chapter 11, while lower than those for United, show that you can't go broke on the cheap. June 28, 2007
Bally Aims for Chapter-11 Fitness
Agreement with subordinated note-holders could pave way for a restructuring that starts next month and is completed 60 days later. June 4, 2007
An ESOP to the Workers
Giving workers a stake in troubled firms may not be the answer. April 13, 2007
Take a Piece of Me
Why shedding parts of a merged entity can make a company whole. April 1, 2007
Restructuring Redux?
Thanks to the availability of cash, the options for bankrupt companies — and their advisers — abound. March 1, 2007
If Daimler ditches Chrysler, whither Detroit? February 20, 2007
Experts Predict Wave of Bankruptcies
"The American consumer is arguably the weakest link in the credit chain," a financial advisor says. November 9, 2006
Burn Prevention for Creditors
Changes to the bankruptcy law make it easier to fend off attempts by insolvent customers (or their creditors) to claw back payments they made before they went belly up. October 31, 2006
Bankruptcy Pay: Will 'Stay' Bonuses Go?
A provision in the new bankruptcy law amendments has companies scurrying to transform executive retention packages into performance-based incentive pay. September 13, 2006
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Autodesk CFD subscribers can now visualise how data changes over time with 3ds Max lighting and rendering tools.
Ideal for nonprogrammers, the Max Creation Graph can be used to:
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They say the ancient Egyptians worshipped cats. I wonder what future humans will say about us when they examine our Internet servers! We all know cats are popular. But, as I found out the other day, they can even help you get more email subscribers, brand loyalty and trust. Yes, I'm being serious.
Guest post written by Ross Kramer Ross Kramer is CEO of Listrak, an email marketing firm. Ross Kramer: Drop them a line. A few weeks ago, I logged onto Amazon to check out prices for a new flat screen TV. I added a few items to my cart to compare the specs. This [...]


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Description: Co-winner of the 1983 National Book Award for Poetry, Country Music is comprised of eighty-eight poems selected from Charles Wright's first four books published between 1970 and 1977. From his first book, The Grave of the Right Hand, to the extraordinary China Trace, this selection of early works represents "Charles Wright's grand passions: his desire to reclaim and redeem a personal past, to make a reckoning with his present, and to conjure the terms by which we might face the future," writes David St. John in the forward. These poems, powerful and moving in their own right, lend richness and insight to Wright's recently collected later works. "In Country Music we see the same explosive imagery, the same dismantled and concentric (or parallel) narratives, the same resolutely spiritual concerns that have become so familiar to us in Wright's more recent poetry," writes St. John.
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All the information you need in one place! Each Study Brief is a summary of one specific subject; facts, figures, and explanations to help you learn faster.
List price: $17.95Edition: 2ndPublisher: Wesleyan University PressPublication date: 12/1/1991Binding: PaperbackPages: 182Size: 6.00" wide x 8.50" long x 0.75" tall Weight: 0.682Language: English
Poet Charles Wright was born on August 25, 1935 in Pickwick Dam, Tennessee. He earned a B. A. at Davidson College and then entered the army. Upon his exit from the service, Wright earned a M. A. at the University of Iowa's Writer's Workshop. Wright is currently a Souder Family Professor of English at the University of Virginia. Wright won the Pulitzer Prize and The Los Angeles Times Book Prize for his work Black Zodiac. Wright has also received the National Book Award for Country Music: Selected Early Poems, and the PEN Translation Prize for The Storm and Other Poems. In addition to the above awards, Wright has also received the American Academy of Arts and Letters Award of Merit Medal and the Ruth Lilly Poetry Prize.
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|Jukka Zitting <jukka.zitt...@gmail.com>
|Re: Jackrabbit OCM after 1.6 status and source?
|Mon, 09 Nov 2009 15:19:00 GMT
Hi, On Mon, Nov 9, 2009 at 12:11 PM, Aleksei Lukin <email@example.com> wrote: > Jukka, please, update web site. You're patches will be much appreciated. :-) Seriously, we only have limited amounts of time and effort to spend, so instead of asking others to do things for you, the best way to get something done is to contribute it yourself. I absolutely agree that the web site and our documentation in general needs quite a bit of more work. If anyone is interested in helping us out with this, I'd love to assist where needed. The wiki is the easiest way to get started as you can just start editing it. I'm also eager to accept suggestions for improvements to the official web site. BR, Jukka Zitting
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|Brian Moseley <...@osafoundation.org>
|Re: jsr-server version bump?
|Wed, 08 Jun 2005 22:20:54 GMT
Brian Moseley wrote: > i just updated to the very latest jackrabbit code and noticed that the > jcr-server api has changed (specifically, DavSessionProvider's > acquireSession() become attachSession()). > > given the api change, can the version number for the jcr-webdav > subproject be bumped? > > thanks! > > ps: WebdavServlet is still using acquireSession. not sure how it builds, > but it certainly throws an exception at runtime. actually, i was confused by some of my old code lying around in a different directory. i see now that jcr-server was massively restructured, so i have some shuffling to do of my extensions. no problem, but it definitely makes the argument stronger for a version bump.
|View raw message


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Lavash is thin, soft flatbread that is served with dips and used for wraps. Lavash can be easily paired with hummus or babagoosh.
What is Lavash?
Lavash is a combination of flour, water, and salt. The bread's thickness differs depending on how flat and thin you roll it out. For extra taste, you can add either toasted sesame or poppy seeds that can be sprinkled on top before baking.
Traditionally, lavash is cooked against the hot walls of a clay oven.
Origins of Lavash
Food experts believe the unleavened flat bread originated in Armenia, while other foodies claim it first surfaced in the Middle East. Regardless where it originated, it's now a popular table bread throughout the Middle East.
Don't wait too long to eat lavash after baking it. This bread dries out quickly. It often becomes brittle and/or hard to chew.
Other Middle East bread and rice recipes can be found here:
What You'll Need
- 1 pkg. yeast
- 1 1/2 cup warm water
- 2 teaspoons sugar
- 4 1/2 cup all purpose flour
- 1 tablespoon toasted sesame seeds
- 1 tablespoon toasted
- 1 1/2 teaspoon salt
How to Make It
Coat a large bowl with oil. Set aside.
In a measuring cup, combine yeast, water and sugar. Mix until yeast is dissolved.
In a large mixing bowl, combine flour and salt. Add yeast water mixture and form a dough. Knead dough by hand for 10 -15 minutes. 5-8 minutes is sufficient if using a knead hook on a mixer.
Once dough is kneaded, place ball of dough in oiled bowl. Roll the dough around the bowl to coat it with oil.
Cover and let rise for 1 -1 1/2 hours, or until dough doubles in size.
Once dough has doubled, punch down to release air. Continue to knead for about 5 minutes.
Divide dough into 8 separate balls of dough. Cover and allow to rise for 30 minutes.
Preheat oven to 400 degrees.
Once risen, roll dough out to thin rectangles, about 12"x10" for large or 8"x6" for small flatbreads. They should be as thin as pizza dough.
Puncture rectangles with a fork. Brush dough with water and sprinkle sesame seeds. Bake on baking sheet for 20 to 25 minutes until golden brown. Continue to bake remaining dough.
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Things to Do on Beachcomber Island
This secluded paradise offers a beachy adventure for visitors of all ages. It's so small that only one resort calls it home, but you can nab a day trip out to the island if you've got a hankering to leap into its clear waters. Kayaking, jet skiing, fishing, paragliding and boating are all big draws here. Land-wise, a white, sandy beach encircles the entire island, while lush vegetation sprawls throughout the middle. You don't have to look far to find a relaxing coconut palm to rest under until the water beckons you once again! Land-based activities include touch rugby, cricket and miniature golf. You can learn to mix your favourite cocktails or take part in limbo and dance competitions. You won't find any roads or rental cars to speed around in on this lush, tranquil getaway; instead, after you book flights to Nadi International Airport—Fiji's main transportation hub—you'll take a ferry to Beachcomber Island and your adventure away from it all, will begin.
If you're ready to embrace the great outdoors, put on some walking shoes and explore the island at your own pace. It's the best way to really get to know the island and learn how the locals live. You won't find any Beachcomber Island car rentals— being such a small island you can stroll around as you need. Besides, what's the rush? You'll be on island time!
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Pakistan Flooding
August 17, 2010 by staff
Pakistan Flooding, The floodwater is Ehsan Ali and his family by surprise. "At first we thought the water was not so serious," says the farmer, 44, who worked in the rice fields that extend throughout the district Shikarpur. "Then suddenly made an announcement in mosques, telling us to run for our lives. A large amount of water that was coming." When the water runs through the farms of the Pakistani province of Sindh, drowning thousands of hectares of crops, tens of thousands of pilgrims every possession they had and fled.
The evacuees now languishing in makeshift shelters. Many have settled on the side of dirt roads, protecting themselves from a scorching sun, to reaffirm a bed on the head or cover under a car. Others are grouped under a sheet of tin by a railway station similar abandoned or decaying school buildings. Doctors say they are already seeing outbreaks of scabies and diarrhea among the displaced. Women have had to go to do work in public places, giving birth in the classroom to share with other families, for example. When they get relief goods provided from a private donation, the struggle is how little panic to gather food that each person can handle in the second available. With each passing day, the anger over government neglect of horses.
It was in an attempt to contain the anger that President Asif Ali Zardari, made a brief visit to Sindh, his home province, last week. Adjustment via helicopter in Sukkur and under heavy guard, the leader glimpsed the devastation, presented checks to the children who suffer, stroking their heads to console them, and then returned to Islamabad. On Sunday, Zardari accompanied by United Nations Secretary General Ban Ki-moon, in southern Punjab, where Ban said: "This has been a day heartbreaking for me … In the past, I have witnessed many natural disasters worldwide, but nothing like this. "Before the floods, Zardari's popularity stood at only 20%. However, it should be on the floor. In the coming weeks, if you want to retrieve the position of his government to be set in Pakistan in a situation where you can begin to rebuild their economies, drawing billions of dollars from the international community and help the millions affected by water back to their lives. Many doubt if the president is up to par.
Last week, World Bank President Robert Zoellick, said the floods had destroyed crops worth approximately one billion. Pakistan By conservative estimates, the figure is at least twice. Some 17 million acres of farmland have been submerged and more than 100,000 animals have died. On the way Rahimabad, buffalo carcases found on the side of the road, rescued by wild dogs under the clouds of flies. Approximately a quarter of Pakistan's economy and almost half of its workforce dependent on agriculture one.
Pakistan's economy was fragile and dependent on a package of 11.3 billion and supported by the International Monetary Fund (IMF). Before the floods, the country was struggling to meet the fiscal requirements, the discipline of the package. Pakistan has a swollen section of the public, a narrow tax base and a chronic balance-of-payments problem. "Now, alter all calculations, all projections, all scenarios," Abdul Hafeez Sheikh, Minister of Finance of Pakistan, tells TIME. "It is too early to fully assess the impact of the disaster, but the damage is colossal, it is still unfolding. Are added billions and billions of dollars." So far, 40 countries have contributed to 222 million, according to figures compiled by the Pakistan government – a fraction of what is needed. The Pakistani Foreign Ministry is still pondering whether to accept the offer from India and 5 million. The UN hopes to raise another 460 million and for Pakistan.
The difficult economic conditions have provided the political opposition a chance. Striking a nationalist pose, former Prime Minister Nawaz Sharif stated that his country does not need foreign aid. Among a wide swath of Pakistani public opinion, holding out a helping hand is a loss of dignity and therefore expensive. Zardari is already overwhelmed by the impression that the West is too dependent on political enemies and believe that your asking the world for help to further damage the president. "It's a nut approach," says an aide to Zardari about political opposition to the aid. "If people are there to help and want to help, why do you want to make them feel uncomfortable?
There is little chance of a change of government. The opposition has to avoid being seen as exploiting a national tragedy, and the army is too busy to resort to a coup. But while Zardari is likely to remain in power, can be left too weak for useful exercise.
The economic consequences of the floods will be felt over the coming months. Not only are the current revenue has been lost, but people also have to bear the higher prices until the economic infrastructure is rebuilt. "There is a massive loss of infrastructure," says Sheikh. "The dams will be repaired in the northwest, not a single bridge has survived along the Indus River, the roads have to be rebuilt, and schools need repair in the field." The damage to the agricultural economy means that food prices are set to rise. "We had plans to export surplus wheat," added Sheikh. "It was an economic opportunity as Russia has suspended wheat exports, increasing its price. We can not export wheat now because we have to feed our own people." observers have raised the grim prospect of food shortages and the resulting civil unrest.
Sheikh insists that the tragedy could offer a glimmer of hope. "It could be an opportunity to make tough decisions," he says. "For example, could push through a sales tax, introduce a charge by the floods in people well and get a scope from the IMF and the international community's support." The reconstruction effort, said the sheikh, may lead to "a stimulus to economic activity," to strengthen the sectors related to construction. For agriculture, the outlook may include the possibility of wells for more water and sediment left by the floods will land in some arable areas.
But for now, there is only destruction and damage. "That's where my land that used to be," says Hamir Soomro, pointing to the mass of surrounding water. For as far as the eye travels in any direction, his family 1,200 hectares of rice and wheat farmland have been submerged. Only the tips of the stems of rice can be seen. In the distance, there are trees and the remains of brick houses collapsed. The village has a strange, haunted feeling of abandonment. Even the police have left the area. "All that is left is a sunset, Soomro said, touching his forehead to get a clearer picture of the full, the orange sun dip into the distance, causing ripples in the water to glow gently.
There was nothing we could do to tame nature's fury, but Soomro said that Zardari's government has deepened the disaster through poor management. A retaining wall was cut supposedly in the wrong direction and instead of water spilling into the desert and snakes its way to the sea, has spread through Shikarpur and other populated areas. Now, with a second wave of floods, the historic city of Jacobabad has been evacuated. In other areas, says Soomro, petty rivalries have been landowners to divert water to other land in the attempts at self-preservation. The government and bureaucracy have failed to coordinate an effort to water misuse.
Soomro and other farmers throughout the country as he will not be able to harvest their crops in one year. "90% of the land is under water," he says. "The rice crop is gone. I have no wheat seed on the left, because that is under water." Usually going to grow wheat in the winter. "Then there is also damage to equipment." Soomro lands employ some 3,000 people and the people of Rahimabad is home to another 7,000 people indirectly depend on the land. In previous years, the crops suffer from chronic water shortages in Pakistan. The heat causes most of the water to evaporate before reaching the roots. Other crops such as sugar cane can not try because they drain the excess water. The irony is that now can take months for it to dry land.
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text:
Manjeshwari P MYSORE
Essence Of Love! - A Sonnet - - Poem by Manjeshwari P MYSORE
The refreshing look that gives the freshness
The lively feeling revives the zest of being alive
The smile that makes you smile, the joy of fullness
The dream that you cherish, want to share and relive
When you stay close to each other
Most happy to forget all the world for a while
There is nothing more important than being together
This is something you have been waiting all the while
Just the way you will know your special someone
Love is about belief, endurance and understanding
Make sure about the happiness of your beloved one
Afterall, Truly in love, closeness means everything
The essence of Love is Oneness
Togetherness brings Happiness!
Comments about Essence Of Love! - A Sonnet - by Manjeshwari P MYSORE
Chinedu Dike (6/2/2015 4:19:00 AM)
The essence of love is indeed oneness. A beautiful piece of poetry, well articulated and insightfully penned with conviction. A lovely poem with nice rhyme scheme. Thanks for sharing. Please read my poem MANDELA - THE IMMORTAL ICON. (Report) Reply
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Stopping By Woods On A Snowy Evening
Robert Frost
A Dream Within A Dream
Edgar Allan Poe


text:
So, I'm planning to do this year's NanoWriMo. My story is going to be a Sci-fi/pirate adventure called 'Arrowhead'
But I will start this on the 1st of November. Until then, I plan to write one creepypasta a day depending on how I feel.
I also have a novella that is currently being proofread but I hope to get back, revised and published before Hallowe'en . It's about vampires. Yes, I know.
I'll probably stop adding new stories after October, and may disappear entirely until next October.