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Donald Trump's Policy Stance Favors Big Banks Like JP Morgan
- Trump's political process involves repealing the Dodd-Frank Act.
- On a net basis this is good for banks and the economy, as lending is the primary driver for M0 currency expansion.
- If Trump gets elected, I would get incrementally aggressive on large-cap banks and favor JPM within the space.
[EDIT: This article tries to analyze the impact of policies of the presumptive Republican nominee on the banking stocks. We at Amigobulls don't have any political leaning and the opinions expressed are those of author. Please read our full disclaimer.]
Trump could pave the path for repealing Dodd Frank
Anything that can impact the fundamentals or performance of a company is what interests me. And with Donald Trump being the presumptive nominee of the GOP party, I have to wonder how some of his potential policies will affect some of the companies I cover. More specifically, his recent comments on repealing the Dodd Frank Act at his San Jose political rally becomes the forefront of our discussion.
I am not a left-wing or right-wing, but I do have a Trump bias, which is something I'm going to disclose before you continue further.
The challenge with the Dodd-Frank bill is quite large, actually. My greatest source of concern comes from the heightened capital reserve requirements. The panacea to preventing the financial system from falling apart could be the very reason why the economy is not recovering as quickly.
Why this is negatively impacting banks
Prior to the enactment of the Dodd-Frank Act, banks were required to carry 3.5% of core common capital as part of a 50% weighting against mortgage securities and 100% weighting for riskier mortgages. Dodd-Frank Act unravels the money multiplier effect, as it requires banks to carry a higher percentage of shareholder equity excluding preferred equity upon the full phase-in of comprehensive capital analysis and review, which requires banks like JP Morgan Chase (NYSE:JPM), Bank of America (NYSE:BAC) and Wells Fargo (NYSE:WFC) to carry higher core equity of roughly 10.5% or so.
The problem with this is that it diminishes the possibility of banks moving lower in the credit tiers, as prior to Dodd-Frank, the big banks were generally more willing to lend. The issue is a little more convoluted, but deregulation for financial sector stocks is broadly positive with the large cap banks gaining much more upside assuming Trump wins the white house.
The broad economic implications are also positive, as it might spark the necessary tinder to drive substantial economic growth. Generally speaking, money is a function of lending, and the faster banks can lend, the monetary base quickly multiplies. This is driven by the money multiplier function.
Prior to Dodd Frank it was estimated that a bank could retain roughly 3% of client deposits and lend out the remaining 97%, which equates into a 33.33 multiplier, which can be computed by dividing a single dollar by .03. The definition of money multiplier is a little different though because I'm making a comparison of required bank equity as opposed to what the bank can lend as a percentage of client deposits.
So, let's dive into the gory details
Generally speaking, the required capital ratios net out the banks liabilities from assets, to arrive at core shareholder equity, thus requiring banks to hold onto more shareholder equity in order to lend money. Basically, upon reaching those minimum capital thresholds, banks can lend more, but it requires that a bank hold onto a higher percentage of all profits before returning to conventional banking. It's hard to make an apple's-to-apple's comparison to the bank deposit multiplier, but when looking at JPM's financial figures, the bank currently has $562.3 billion in client deposits and $445.8 billion in total loans within its consumer banking segment.
This implies that $116.5 billion in client deposits is not being utilized, and the remaining $445.8 billion is lent out, of which JPM backs a decent chunk with its core equity. Basically, the implied money multiplier gets diminished quite significantly, because the bank is holding onto roughly 26.1% of client deposits as a proportion of money getting lent out. This has been the case at a lot of the big banks and many are not lending to those belonging to lower credit tiers.
If JPM's tendency to shore up capital happened at every bank, we'd be operating a money multiplier effect off of a 26.1% reserve ratio, which implies a 3.8x multiplier. Not good, not for the broader economy, because if we assume banks are underutilizing client assets, I then have to assume deflationary headwinds as inflation is a function of banks propensity to lend as a percentage of all client deposits. Basically, if the big banking franchises reduce lending any further, which will happen upon the full phase-in of capital requirements derived by Basel 3, which is provisioned by the Collin Amendment from the Dodd Frank Act, the monetary base will likely contract.
Source: Federal Reserve
When looking at the money supply, the figures aren't looking good. Between 2014 and 2016 the bigger banks have acted quickly to deleverage their balance sheets ahead of fully phased-in requirements by 2019. In fact, many of the bigger banks have moved even quicker to reach those minimum capital requirements, so they can return cash back to shareholders.
When looking at financial sector aggregated statistics, the loan/deposit ratio hovers at around 75%, which confirms the observation of JPM's financial figures, but also industry-wide. If I had to imagine a reason for why CPI metrics aren't normalizing above 2%, this is probably why.
So, you'd imagine that if banks were to operate at higher capital ratios, that they would continue to add cash to their balance sheet so they can lend more money to consumers, right? Technically, banks can lend more or utilize a lower reserve ratio if they were to hold more cash on the balance sheet. But instead, banks are opting to return any excess above the 10.5% core equity back to shareholders via dividends and share repurchase, which is money not going back into the economy via conventional lending.
This explains why central bank interest rates have had modest impact on lending, and inflation. If banks can't lend, money can't multiply, which has nasty implications for inflation. This economic truism can't be altered even if you're a Paul Krugman or Janet Yellen in the economist community. Anyone who understands banking, regulation and economics knows that the banks aren't lending as much to consumers and are retaining deposits at unprecedented rates.
The average credit score is still higher than the prior cycle by a significant margin. Many of the consumers in the United States can't borrow at a 750 to 760 credit tier (yet this is the average). In other words, tight underwriting standards compounded by regulation that requires higher capital reserves equates into slow consumer loan growth.
Final thoughts and conclusion
I find it highly improbable that consumer banking will make a substantial comeback until the Dodd-Frank Act is removed. While the act favors small banks, it really ignores the reality that much of the banking sector is dominated by large cap franchises. In other words, if you really want an economic recovery you're likely to get one if Dodd-Frank is repealed.
If Trump wins the White House, the odds are quite high that big banks will do even better. Of the big banks, investors should consider JPMorgan as its core franchise remains strong, and fundamentals will likely drive the stock to $73.93. The entire bank sector may have even more upside though if Trump gets elected for president by year-end. At that point, I may even revise my price target higher. I think every bank analyst is crossing their fingers on this because repealing
I think every bank analyst is crossing their fingers on this because repealing Dodd-Frank would be an even bigger catalyst than low interest rates, asset purchases by the Fed, or other fiscal/monetary policies. If banks lend with fewer restriction, the economy will likely normalize to historical nominal GDP growth rates.
In a future article, I'll be covering how a Trump's presidency will affect banks in even greater detail.


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"How did it get to this point? Why am I working, working, practical next to my boss lint in my business and fear as though I don't have a instant to focus ahead?"
You in all probability started your own business to have state of choice, to have state to build and to have freedom to have unrestricted proceeds in need the constraints of a pay.
You may have vanished a J.O.B. and now you are fear as nevertheless you are erstwhile once again at a J.O.B. near your most unsuitable boss of all time...YOU!Post ads:
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Creating a association that others can run in need you can elasticity you that state that was your purpose to having your own conglomerate. This takes subject field and thorough glare of publicity to all the details that you are soon handling.
Oh, how seductive it is to feel you can touch it all! Maybe you give attention to you status to get large and have more earnings formerly you be to have the structure you need?
It may seem to be unsafe to be paid for the post when your monetary resource are tight, but in need the back in put down you are apt to act stranded where on earth you are.
You are too a perfect nominee for labor and throw. When you are overworked and overwhelmed, the feeling that player you into the business organization starts to decrease and die.
Ask yourself, "How masses work time am I really practical in my concern all week?".
Would you ever have worked that many hours in ANY job you've of all time had?
I didn't construe so.
The resist facing maximum enterprise owners is how to come up beside the case to slog ON the business concern so that they can upgrade the manoeuvre of their establishment.
Now it's clip to appearance for the instance wasters that swipe every of your precious occurrence distant. Is it email? That is a challenge for lots of us.
How jammy it is to get sucked into checking email and past get unclear by any figure of riveting topics.
Disciplining yourself to watch email lone at sure present of the day could freed up every semiprecious example for your company readying.
Another obstruction to our prosperity can be avoiding thing (like busines preparation) because it seems too outstanding of a hang over. If you can stop the extend beyond low into minor segments, it will be easier to fit the slog on one of the segments into any unconfined juncture that you have.
Actually creating appointments near YOURSELF for a translation would be a way to compile juncture for this company preparation.
And bread and butter that appointment!
Show yourself and your commercial as noticeably obsequiousness as you variety the remains of the international.
If you have snags in disciplining yourself into devising several of these changes and creating a commercial plan, it may be the circumstance to advisement just about hiring a company handler.
A company trainer would be competent to crutch you in feat done this change of state into a systemized enterprise structure that will permit you to turn fruitfully and to have the freedom and invention you wanted in the original place!

subset: commoncrawl


text: Oh My God! How do I describe the Champions League Final. The tears of joy from Ronaldo. The tears of sorrow from captain Terry. One can only sympathise with him. He had a 12 yard kick to win the title only to slip and watch the ball hit the post to give united a life line. The turning point of the game for me however was Drogba's red card which was totally unnecessary. It would have been him taking the kick and in all probability not Terry. Giggs could have sealed it in extra time but for Terry's intervention. Penalties it would have to be, with United kicking first. Tevez and Carrick both scored and then came the shocking miss from all people United. Chelsea kept scoring and then it all came down to the last kick with Terry needing to score to win. Heavy rain and memories of Beckham's penalty came back when he slipped and missed. Given the lifeline United capitalised and it was Nicholas Anelka who turned villain missing and giving United and Ferguson their second European Championship.United dominated in the first half where as Chelsea got them in the second. So final score ended 1-1 with Ronaldo and Lampard scoring and 6-5 in the penalty shootout. This was Ferguson's first win in a penalty shoot out as a manager and now we all wonder whether Grant goes with packed bags.


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New Delhi: Costa Rica coach Alexandre Borges Guimaraes says his team would try to emulate his team's performance in Italy 1990 at the 2006 World Cup in Germany. "This World Cup is a very good opportunity for us, to the national team, because we have almost the same players that we have in the last World Cup in Korea-Japan and we are trying to emulate what we did in Italy 1990," said Guimaraes. Brazil-born Guimaraes moved to Costa Rican at the age of eleven to become one of the most loved football figures in Costa Rica's history. Making the national squad as a player, he played a pivotal role in the Costa Rican's 1990 World Cup crusade. He was made the coach for the 2006 team following the deposition of coach Horge Luis Pinto. Pinto's tenure saw a string of poor performances from the Costa Ricans and at one time they looked like they would not even qualify for the World Cup. But with Guimaraes at the helm, Costa Rica went on to defeat Guatemala, Panama, Trinidad & Tobago and the US at home to secure a berth in Germany, finishing with five wins, a draw and four losses.
Topics : Football
Get the latest ICC Women's World Cup 2017 news, check ICC Women's World Cup 2017 schedule, WWC live score & WWC Points Table. Like us on Facebook or follow us on Twitter for more ICC Champions Trophy updates. You can also download the NDTV Cricket app for Android or iOS
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NEW ORLEANS (AP) — BP's massive oil spill is ready to become the largest ever in the Gulf of Mexico.
It's an ominous record that underscores the oil giant's dire need to halt the gusher.
Based on the highest of the federal government's estimates, the oil that's gushed for two and a half months from a blown-out well a mile (two kilometers) under the sea is expected to surpass the 140 million gallon (530 million liter) mark on Thursday.
That eclipses the Ixtoc I spill off Mexico's coast in 1979 to 1980. Even by the lower end of the government's estimates, at least 71.2 million gallons (270 million liters) are in the Gulf.
That calculation is based on the higher end of the government's range of barrels leaked per day, minus the amount BP says it has collected from the blown-out well using two containment systems.


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Midnight City M83 Lyrics MP3
M83: "Midnight City" Official Lyrics MP3
We do not own this song. This video is for the sole intention of sharing lyrics.
M83 'Midnight City' Official video MP3
News: Listen to the new M83 track 'Do It, Try It', taken from the new album 'Junk': http://m83.it/junkYo M83 is touring through 2016: http://m83.it/ticketsYo Watch ...
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LYRICS* Waiting in a car Waiting for a ride in the dark The night city grows Look and see her eyes, they glow Waiting in a car Waiting for a ride in the dark ...
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Watch in HD for the best screen quality, otherwise it's terrible!:/ Lyrics in case you think they're wrong: http://www.songlyrics.com/m83/midnight-city-lyrics/
M83 // Midnight City (Lyrics) MP3
No Copyright Intended*
Midnight City (HQ) - M83 Remix by YONAS (Lyrics in desc.) MP3
lyrics; Life faith, I Take a look at the clock "and see I'm down on my final hour" "I'm talkin' about life, faith, I ( I see ya bout to do it on this one) "take a look at the ...
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Nach langem mal wieder ein Video gemacht & hochgeladen :) Ich hoffe es gefällt euch :)
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Midnight City by M83 hope you enjoy sorry about the powtoon ad thingy.
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I do not own this song M83 Website: http://ilovem83.com/ (My first lyric video)
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Artist: M83 Title: Midnight City Tuneload:http://www.mediafire.com/download/8v9myjbv1w8halo/M83_-_Midnight_City.mp3 Lyrics: Waiting in a car Waiting for a ...


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A good hotel for individual travellers, with the shopping centre within walking distance and in a fantastic location right in the centre of the town.0708 hm
This hotel is situated in a quiet side street, just off the major shopping centre. Konigsallee and Central Station are within walking distance.
This is a traditional, white building in a residental area.
This lobby is small. The bar and reception are within the same area.


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(First Ever) Red Stick Food Swap
Red Stick Food Swap
Tuesday, December 10, 2013 from 6:30 PM to 8:30 PM (CST)
Handmade Food & Craft Swap
Kick-off the holiday season by swapping homemade & handcrafted goodies! All swap items must be homemade, homegrown, foraged or handcrafted by you.
Ticket Policy
You must register and have a ticket to participate in this event. Each swapper with a ticket can bring 1 guest to enjoy the event. Please do not bring more than one guest per swapper. Your guest does not need a ticket.
By "purchasing" a free ticket you agree to the following:
1) I will adhere to the highest food safety and cleanliness standards in food preparation.
2) I will label all food and beauty items with an expiration date and storage instructions, if applicable.
3) I will bring nothing that has been canned prior to November 2012.
4) I will contact Red Stick Food Swap at email@example.com if I can not attend so that my ticket can be given to the next swapper on the waitlist.
5) I will assume all liability; specifically I agree to not hold liable food swap hosts, swap venue owners/providers, or other swap participants.
6:30-7:00: sign in, set up swap items & samples, fill out swap sheets
7:00-7:30: browse offerings & sample goods; enjoy refreshments/samples
7:30-8:00: write trade offers on others' sheets
8:00-8:30: review swap sheet offers, find each other & swap away!
Please arrive no later than 6:45pm
Helpful Notes
You can bring a bunch of one thing or multiples of a few different things. It's up to you!
Please be sure to practice good food-safety standards. Take precautions to adequately protect your food while it is in transport, and while it is at the swap, if needed (i.e. a cooler).
We will provide swap sheets, name tags, writing utensils, and other necessary materials for the event. If you are providing samples, please bring everything necessary to let other sample your items.
Before you go
Bringing snacks/samples to share?
Great! Make sure to bring any serving utensils/platters you might need. Please label them on the bottom to make sure you get them back.
At the swap
How to swap
After the swap
For more information about our homemade food & craft swap, visit our website at www.redstickfoodswap.wordpress.com
Join millions of people on Eventbrite.
In order to purchase these tickets in installments, you'll need an Eventbrite account. Log in or sign up for a free account to continue.


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Fri, 18 Dec 2009 /
Sat, 19 Dec 2009 /
Thu, 31 Dec 2009 /
The votes are in: RA reveals the best long-players of the year.
The RA staff highlights the finest mixes and compilations of the year.
RA's staff chooses their favourite imprints of the year.
Laptops, hardware, saxophones, oh my! The RA readers choose their top live acts of the year.
RA's readers start the poll season off by picking out their favourite DJs of the year.


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Resources for HIV Providers to Stay Informed and Take Action on ACA Repeal Efforts.
A resource supported by the HIV Medicine Association
February 13, 2017
Resources for HIV Providers to Stay Informed and Take Action on ACA Repeal Efforts.
November 5, 2015
Are you are aware of any access issues to Daraprim (pyrimethamine) since the price increase by Turing Pharmaceuticals? If so, share your comments on our blog.
Resources to help providers improve access to hepatitis C treatment for their patients.
Designed to help clinicians properly bill for outpatient HIV services, HIVMA created a pocket guide, wall poster and accompanying webinar. The guides include reference lookups for patient history, physical exam, table of risk and medical decision making.
HIVClinician.org was created to help HIV medical providers and clinics adapt to health care financing and system reforms. It is a project of the HIV Medicine Association.


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So, periodically, I'm gonna post a creative diary of sorts on my upcoming project Your Ass Is Grass. This is number one.
Work on the script has been going well, but there've been a few hitches here and there, as always. First and foremost, I decided to put the script away for a bit to let it jell in my head a little more. My writing process is pretty clumsy: The first draft is to figure out what my story is going to be; the second and third are simply trying to make those elements even better — to find out what really works, what doesn't, and to make the elements that do work richer, more 'themselves,' so I can truly create an excellent and original piece of work.
That's a slow process, but for me a necessary one. I've never been able to outline and proceed simply with that, due to the fact that most of my creative decisions that stick seem to be the ones made in the heat of composing. Thus, coolly sitting down to outline a story seems to be too easy. There needs to be blood on the page, a heady mixture of plot and character that comes only from the thick of battle.
Second, my script The Mourning Portrait has gone through a few small revisions, so I jumped onto that project for a time and let YAIG sit and jell for just a bit. Which is okay. That's life. If you've read my previous post about the writing process, you know that as long as progress is being made on some front, I'm happy. The key when you switch from project to project is to keep that momentum and don't falter. Easier said than done, of course.
But the script for YAIG is coming along nicely — in my head. Sometimes it helps not to work too fast, or to try to hurry things. I really do think the extra time is often well spent. One of the bad habits I'm trying to get rid of is to rush things along, and honestly, to write scripts that could have been better if I had taken an extra four months or so to finish. That's why I'm taking my time and letting themes and ideas simmer before committing them to a final draft.* This is a good one, I'm telling myself; one of the ones I'll be remembered for. So I'm in no hurry to get it out there.
Thanks for your interest! Keep your butt in the chair!
*This is not to contradict my earlier assertion that work is best done only staring at the page. There are two kinds of writing, of course: the actual compositional kind, and the dreamy, staring at the ceiling kind. Both are essential.


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THE race for the second promotion spot in division two of the IT Sports Mixed Tennis League is going down to the wire.
Wetherby Castlegarth captured the division two title weeks ago and ended their campaign with a win over Bubwith. Top scorers Angela Crossley and Tim Purdy helped to relegate Bubwith, unlucky to go down having won five matches.
Four sides are still bidding for promotion, with Fulford now in second place with a games difference advantage of one over Poppleton.
Fulford lost the chance to clinch the runners-up spot after going down to promotion rivals Rowntree Park, but ended with a good win thanks to unbeaten Faye Garland and Tom Craddock.
York have won their last two matches to stay in the promotion hunt as unbeaten Howard Pallister partnered Tricia Urquhart in a victory over Rowntree Park and then Margaret Whitehead in a triumph at Poppleton.
Sutton-on-Derwent's rearranged matches will decide whether they are promoted in division three after Cliffe lost their final match at Stillington, who were boosted by the unbeaten father and daughter team of Rozzy Carrick and Tim Madgwick.
Bishopthorpe grabbed the chance to move into second as Ellie Page and Jamie Winspear won 29 games against Fulford. Fulford will be relegated with Copmanthorpe, who lost to a Dunnington side which had Rosie Todd and Greg Ashcroft winning 31 games.
York are champions of division five after Katie Shorthouse and Dave Howarth led the scoring against second-placed Riccall.
Lorraine Bishop and David Welsh spearheaded a good win by Cawood to save themselves from the drop but relegate Poppleton.
Bishopthorpe could find no answer to the 26 games won by Wetherby's Helen and Stuart Robinson, but they are in third place, ahead of Castlegarth on games difference.
Appleton Roebuck are the new division six leaders after beating Stamford Bridge, with Emma Robertshaw and David Luscombe winning 27 games.
Bishopthorpe 3 have to win their remaining two matches to take the division nine trophy after closest rivals Heworth beat Cliffe with the aid of captain Helen Walker and Mike Boothman.
Jackie Mason and Robert Field won 28 games at Fulford to keep Bishopthorpe in top spot.
New Earswick's Nicky Georgiou and Keith Pearson won 36 games at Sutton-on-Derwent in division ten.
Liz Walker and Neil Pratt ensured Wilberfoss 3 would be promoted by winning 27 games at Dunnington.


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Vicent Martín i SolerValència 1754 - Sant Petersburg 1806
This Valencian figure was one of the most famous opera composers towards the end of the 18th century. Known as Martini "lo spagnuolo", he completed his music studies in Italy and rivalled Mozart in Vienna. He was later send to St. Petersburg, where we was to helped establish the operatic tradition at the court of the Tsar.
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The aria in the singing lesson
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text:
Words! (8 of 12) by Jerry Vines
This content is part of a series.WORDS! (8 of 12)
Proverbs for Parents
Jerry Vines
Proverbs 12
I want to talk to you about words for a little while
this morning. "Sticks and stones may break my bones,
but words will never hurt me." Have you ever heard
that? Probably you learned that, like it did, when you
were real young and going through the experience of
school. Somebody said something to you that was pretty
brutal or very unkind and you have been taught to
reply, "Sticks and stones may break my bones, but
words will never hurt me."
Tell that to Erica Harrold, our new Miss America.
There was an interesting article this week about her
in the newspaper. It said that the new Miss America
was picked on and threatened by her fellow students in
the 9th grade. It says that she was called a variety
of unmentionable names in the 9th grade. She came from
a multi-cultural background. She received anonymous
telephone threats against her life. Eggs were thrown
against her family home. Somebody mysteriously cut off
their power one night. She didn't go to a
stereotypical poor or high crime institution. Quite
the opposite. She went to a school for gifted
students. When her parents transferred her to a
conventional public high school, her harassment
problems evaporated. She went on to graduate with
honors from the University of Illinois and has been
accepted by the Harvard Law School.
"Sticks and stones may break my bones, but words will
never hurt me." The truth of the matter is - words can
hurt you. You never forget them.
"Word will never hurt me." Say that to this 4th grader
whose parent brought James Dobson a note that was
placed on her desk. "Awful Susan: You are the
stinkiest girl in the world. I hope you die, but I
suppose that's impossible. I've got some ideas - 1.
Play in the road; 2. Cut your throat; 3. Drink poison;
4. Get drunk; 5. Knife yourself. Please do this, you
ugly girl. We all hate you. I'm praying, O ...
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text:
Longtime member of UAS English department passed away on March 22
By: Don Cecil, former English faculty
Jo Devine-Acres, a member of the UAS English department for more than 20 years, passed away on March 22 due to an inoperable brain tumor. She taught a wide variety of courses on the Juneau campus, but her specialty was expository writing. She served on many committees related to student writing, but she was most proud of her work related to UAS's regional on writing assessment program. She was one of a handful of faculty in the late 1980s who researched writing assessment at other universities around the country and built a very successful lower-level student portfolio review program for UAS. During the program's early portfolio reading sessions, it was obvious to some of us that Jo's students overall were undertaking more complex writing tasks and carrying them out at a higher level, which immediately caused those of us with lower expectations for our students to "up" our expectations significantly—a major benefit of the program.
Those English faculty who knew Jo well will remember her as a steadfast champion of the community college mission and lower-division education. A prolific reader, she loved to expose her lower-division students to new and challenging ideas for the purpose of generating writing that allowed students to practice clear and independent thinking. Most of all, Jo was a dedicated and passionate colleague who loved to talk about teaching and who participated vigorously in the life of the department.
Although she was only 60 at the time of her death, Jo was fortunate to be able to enjoy several good years of retirement on Whidbey Island at her cabin, where she spent most of her time reading, gardening, and visiting with her grandchildren. She is survived by her husband, Greg Acres; her two sons, Bradley and Mason Leland; and her six grandchildren.


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'Hunger Games' Star on Record Breaking Opening: 'We Are Still In Shock' (EXCLUSIVE)
Hunger Games star Isabelle Fuhrman is still reeling over their record breaking weekend (and Monday, FYI). So how did she find out it was the third highest opening in history?
"One of my friends actually called me!" Isabelle told Celebuzz. "I got a bunch of emails from my agent and from a lot… More »


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This is a studio complex that consists of 18 fully-equipped rooms. The dominating colours of blue and white create a hospitable atmosphere. There is a lobby at this city hotel and 24-hour reception and check-out services and a parking area are available.
The complex is located a distance of 800 metres from the port of Milos while the centre of the village of Adamas, where there are also public transport links, is 450 metres away. Shopping facilities and bars are located within a short walking distance. Milos Airport is about 5 km from the hotel.
All the rooms are decorated based on the traditional Cycladic architecture and provide a double bed, air conditioning, a private en suite bathroom with a shower, television, fridge, hot water all day and everyday cleaning services. There are also tea and coffee making facilities, individually regulated heating and a balcony or terrace for each room.
The nearby beach is sandy.
Room Facilities:
Mini fridge
Tea Coffee
Double bed
Individual airconditioned
Individual heating


text:
a4boston Review
Does exactly as it should
A decent basic rug pad for a good price. Shipped fast and was exactly as described.
- Did the color match the color displayed onsite? Yes
- How is the quality of the product? Good
- Did the description onsite accurately describe the product? Yes
Was This Review Helpful?


text: We had already heard that the Samsung Galaxy S IV would not be launching at the upcoming MWC event in Barcelona, so the big question was just when would it launch? One industry insider is claiming he has the date, not to mention the location.Eldar Murtazin, founder of Mobile-review and a man who tends to get it right more often than wrong, claims 14 March is the big launch date. He didn't actually say the Galaxy S IV, but his cryptic Tweet of a 'big announcement' is widely being read as the launch date for Samsung's next flagship smartphone.That's about two weeks after MWC, giving Samsung plenty of space from any launches at that event. After all, no one wants to be overshadowed. Saying that, Murtazin's Tweet does hint at the S IV taking the wind out of HTC's sails. The S IV will appear not long after HTC's flagship, the HTC One, gets its official launch at MWC and around the time it will be pitching for retail sales.New York is said to be the location officially, although Samsung might well run events in different territories, including a London event. After all, this is the big one for Samsung.If previous rumours are to be believed, the Galaxy S IV is rumoured to feature an Exynos 5 Octa (8-Core) processor, Mali-T678 (8-Core) graphics, a 4.99-inch SuperAMOLED Full HD Resolution Display, 2GB RAM, 13-megapixel rear facing camera capable of shooting 1080p Full HD video at 30FPS, 2-megapixel front facing camera capable of shooting 720p HD Videos and Android 4.2.2 Jelly Bean for the OS.Expect official confirmation of the event (if, of course, this is true), in the coming weeks.Source: SamMobile


text: Milton surface wipes are specially formulated to clean and protect from germs. They are ideal for quick clean ups around the house, or when away from home. Suitable to use on high chairs, changing mats, toys, toilets, bins potties, door handles etc. The wipes can also be used to disinfect kitchen items or kitchen hygiene surfaces. The wipes are impregnated with an antibacterial and anti-fungi solution which doesn't leave any residue or stains after drying.


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Various ArtistsLabel: Rhino
Release Date: 08 September 2014
Format: LP Double Vinyl
|Various Artists SATURDAY NIGHT FEVER (ORIGINAL MOVIE SOUNDTRACK) Limited Reissue Vinyl 2LP Set
Saturday Night Fever: The Original Movie Sound Track epitomized the disco phenomenon of the late 70s. Composed and performed primarily by the Bee Gees the soundtrack became synonymous with the classic film starring John Travolta. It created huge hits for the Bee Gees with tracks like Stayin' Alive, Night Fever, More Than a Woman, Jive Talkin and You Should Be Dancing and in 2012 was ranked no. 132 on Rolling Stone magazine's list of the 500 greatest albums of all time.
Featured Tracks:
Side 1:
1. Stayin' Alive Bee Gees
2. How Deep Is Your Love Bee Gees
3. Night Fever Bee Gees
4. More Than A Woman Bee Gees
5. If I Can't Have You Yvonne Elliman
Side 2:
1. A Fifth Of Beethoven Walter Murphy
2. More Than A Woman - Tavares
3. Manhattan Skyline David Shire
4. Calypso Breakdown Ralph MacDonald
5. Boogie Shoes - K.C & the Sunshine Band
Side 3:
1. Night On Disco Mountain David Shire
2. Open Sesame Kool & the Gang
3. Jive Talkin' Bee Gees
4. You Should Be Dancing Bee Gees
Side 4:
1. Salsation David Shire
2. K-Jee M.F.S.B


text:
Elena Bouryka
Sword of War
During the 12th century, German Emperor Federico Barbarossa (Rutger Hauer) triumphs over enemy lands to revitalize a once-great empire. But he must contend with Italian Alberto da Giussana (Raz Degan) and his "Company of Death," which is comprised of 900 angry men. They are all willing to fight to the death at the epic Battle of Legnano. Renzo Martinelli (The Stone Merchant) directs and co-writes this history-rich drama.


text:
The majority of wine produced in England (around 90 per cent) is white, as the
British climate rarely reaches the temperatures needed to ripen red wine
grapes. Here, Peter Hall, the owner of Breaky Bottom vineyard in Sussex, is
pictured with some of his vines. The vineyard specialises in white and
sparkling wines.
English wine beats champagne to win Decanter trophy
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text:
Significant amounts of carbon dioxide have been dissociated by a single flash of a xenon-filled quartz flash tube. For any one piece of quartz, the effectiveness of a discharge varied as expected with the various parameters. Considerable variation was found between any two pieces of quartz. The maximum output encountered was equivalent in the spectral region used to a blackbody of about 8000°K.
© 1960 Optical Society of AmericaFull Article | PDF Article
OSA Recommended Articles
R. E. Huffman, Y. Tanaka, and J. C. Larrabee
Appl. Opt. 2(6) 617-623 (1963)
B. C. Roquitte
Appl. Opt. 6(3) 415-416 (1967)
P. G. Wilkinson and E. T. Byram
Appl. Opt. 4(5) 581-588 (1965)


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FedEx Fleet Types
Flipping through various shoots looking for something else, I happened to come across a few shots of aircraft from the FedEx fleet. It occurred to me that I could drag together a post that was focused purely on the FedEx … Continue reading


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text: Politics 27 March 2008 Rasputin in a tank top A fashion faux pas by the No 10 enforcer and a setback for Lord Moneybags's media ambitions Sign up for our weekly email * The first sighting has been recorded of the No 10 enforcer Stephen Carter in one of his Frank Spencer tank tops, albeit with a Ralph Lauren logo. Talibrown veterans seething over this alleged crime against new Labour fashion, or more trivial issues such as Carter's growing influence, may care to pause before whingeing to Uncle Gordie. A quiet word in the Supreme Leader's ear or a begging letter could be safe, but a hostile email might trigger involuntary early retirement. Carter is seeking a Rasputin-like grip over Brown after securing full access to the Prime Minister's emails, in as well as out. Perhaps he learned the value of control during a private two-hour tête-à-tête in Brussels with the Prince of Darkness, Peter Mandelson. Lord Ashcroft of Belize's dream of becoming a media mogul has suffered a big knock, Labour MPs vowing to boycott the Tory deputy chair's freebie rag, Total Politics. In excess of a dozen immediately signed a Commons motion tabled by the Scouse bruiser Peter "Killa" Kilfoyle to reject unsolicited copies of the Conservative peer's organ. Half the potential readers binning Total Ashcroft unopened would test the depth even of Lord Moneybags's voluminous pockets, never mind the resolve of advertisers promised a cross-party readership. It's official: MPs are certifiable. Speaker Michael Martin is to issue certificates to former MPs, thanking them for their services to expenses. The embossed sheets, with the portcullis crest and mace in raised gold, are described as a "nice family heirloom". After the row over claims on the public purse, many voters will feel former MPs already live in a nice family heirloom - bought by taxpayers through generous second-home allowances. The Bedfordshire belle Nadine "Mad Nad" Dorries is the first virtual MP. Dodging a question from a lobby scribbler, the one-time nurse replied that she'd issue a statement on her website if she had anything worthwhile to say. An unkind Con colleague, irritated by Mad Nad's tendency to boast about how attractive red-blooded MPs find her, loudly suggested she'd disappear completely if true to her word. Knives are out for Hattie Harperson after she had a Labour NEC meeting put back an hour then failed to attend. Word is Sister Hattie also lobbied a member to postpone a decision on whether Wolverhampton North-East replaces retiring MP Ken Purchase from a women-only shortlist. Curious. I thought her hubby Jack Dromey wasn't interested in a Midlands constituency. Kevin Maguire is associate editor (politics) of the Daily Mirror Kevin Maguire is Associate Editor (Politics) on the Daily Mirror and author of our Commons Confidential column on the high politics and low life in Westminster. An award-winning journalist, he is in frequent demand on television and radio and co-authored a book on great parliamentary scandals. He was formerly Chief Reporter on the Guardian and Labour Correspondent on the Daily Telegraph. Subscribe from just £1 per issue This article first appeared in the 31 March 2008 issue of the New Statesman, Is Boris a fake? More


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The bench mark price of ethanol has been fixed at Rs44 per litre and the government had made it mandatory for OMCs to blend 5% ethanol with petrol, which has now been increased to 10% but actual lifting has been unsatisfactory
Oil Minister, Dharmendra Pradhan, admitted in the Lok Sabah recently, that the OMCs (Oil Marketing Companies) were able to "achieve" only a 1.37% blending of ethanol with petrol against the target of 5% which, had recently been increased to 10%.
The Indian Sugar Mills Association (ISMA) had assured the government of their willingness to reach 10% blend level across the country, but sought flexible ethanol blending with petrol, ranging from 5 to 25%, depending upon sugar cane availability.
The sugar mills are currently realising about Rs32 per kg for sugar.They can produce only 95kgs of sugar for every tonne of cane crushed and 45kgs of molasses yielding about 10.8 litres of ethanol. However, if the entire juice from cane is used for fermenting into alcohol, there would be
no production of sugar but 72 litres of ethanol could be obtained.
The bench mark price of ethanol has been fixed at Rs44 per litre and the government has made it mandatory for OMCs to blend 5% ethanol with petrol/diesel, which, as reported above, had now been increased to 10% but actual lifting has been unsatisfactory. Moneylife had reported earlier, that while the sugar mills had a stock of 65 crore litres of ethanol, only 35 crore litres had been lifted by OMCs, resulting in huge blocking of funds. If such a situation should continue, nothing prevents state governments from permitting sugar mills to convert sugar cane juice to alcohol.
It has been reported in the press that higher ethanol blending would hit the liquor industry, a fear that may have caused lower blending rate.OMCs must realize their responsibility and ensure that they lift ethanol from mills on a regular basis and also make available blended petrol/diesel at all
government transport depots besides all major bus terminals. In fact, every pumping stations must be asked to ensure availability blended petrol/diesel so that the consumption is increased.
The ISMA, in a press report, has stated that the estimate for the current season, 2014-15 is expected to be 25.3 million tonnes of sugar, a mere 4% increase over last year, with both UP and Tamil Nadu showing a shortfall but offset by increase in Maharashtra, Karnataka and Gujarat. The carryover sugar stock at 7.5 million tonnes is more than the required buffer and should take us through comfortably to the next season, even after providing for exports.
Luckily, the monsoon's progress, as advised by IMD (Indian Metrological Department) shows that they expect the rainfall to be well distributed and there will be adequate showers throughout the country, which will reduce the deficit predicted earlier due to an El Nino effect.
In the meantime, it is imperative for the government to take serious steps against the OMCs for not complying the required blending programme that have been made mandatory. One way of enforcing such a rule is to tell the OMCs that unless they lift and blend ethanol from mills, they won't be given the subsidies committed by the government.
Such a threat alone will work with OMCs, who must also be forced to focus on setting up ethanol blended petrol/diesel units in all the pumping stations throughout the country. As a start, this could be enforced in all the sugarcane producing states where the mills are established. If such actions are not taken, sugar mills may start looking for export market to sell their ethanol.
(AK Ramdas has worked with the Engineering Export Promotion Council of the ministry of commerce. He was also associated with various committees of the Council. His international career took him to places like Beirut, Kuwait and Dubai at a time when these were small trading outposts; and later to the US.)


text:
Auditor eyes spending at other public bodies
After highlighting lavish spending at the Equal Opportunities Commission, the Director of Audit said yesterday that other subsidised organisations and statutory bodies would be scrutinised.
And he did not rule out conducting value-for-money investigations on the Chief Executive's Office and major government bureaus.
The comments by Benjamin Tang Kwok-bun came as the government said it would listen to views from lawmakers and the public before deciding whether to renew the contract of EOC chairman Raymond Tang Yee-bong, due to expire next year.
'The investigations in the past few years have shown many [public bodies] have poor understanding of what constitutes good corporate governance. They also did not have good systems for handling money,' Benjamin Tang said in an RTHK interview.
'The issue of good corporate governance is starting to be a problem.'
In its latest six-monthly report, the Audit Commission criticised spending at the EOC, ranging from a HK$15,200 dinner in Beijing to paying a HK$320 speeding ticket, and buying 32 air purifiers for its office at a cost of HK$78,000.
The report put the spotlight on Raymond Tang for his generous hospitality and acceptance of hospitality items while on trips abroad. It said his life-insurance package, the premium for which last year cost HK$24,600, was not authorised by his contract.
Mr Tang has denied the EOC has done anything wrong and rejected charges it was a big spender. He has also dismissed suggestions that he should resign.
Benjamin Tang denied the Audit Commission was running a campaign of reprisal against public bodies, saying he was only trying to 'help them save money' and improve corporate governance.
Under his leadership since 2003, the Audit Commission has increasingly targeted public bodies and has issued a series of damning reports against organisations including RTHK, the Tourism Board, the Consumer Council and the English Schools Foundation.
Democratic Party vice-chairwoman Emily Lau Wai-hing said: 'Although it is right to expose big spenders like the EOC, the Audit Commission should also hunt some big tigers in government bureaus rather than giving people the impression of it being a government hit man.'
On an official visit to Guangzhou, Secretary for Constitutional and Mainland Affairs Stephen Lam Sui-lung, whose bureau has a portfolio dealing with the EOC, said the government was considering contractual matters regarding its chairman.
Asked whether Raymond Tang's contract would be renewed when it expired next year, Mr Lam said the government would 'listen very carefully' to views from the legislature, new board members of the EOC, and members of the public before making a decision. He said he had already written to Mr Tang urging the EOC to implement recommendations by the Audit Commission.
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While Pokémon Go has already generated millions of dollars for developer Niantic and Nintendo, it could always do better. Niantic has revealed that it is looking at the idea of sponsored locations.
In an interview with the Financial Times, Chief Executive John Hanke said, "There is a second component to our business model at Niantic, which is this concept of sponsored locations." This seems like a fitting way of generating some extra revenue, as businesses are already investing in lures as a way of attracting players to their location.
Advertisers would be charged for every customer visiting a store, as confirmed by the Pokémon Go app.
Niantic is familiar with this concept, having previously worked with companies to turn their locations into portals in its other AR game, Ingress. It was careful when introducing these sponsorships in Ingress, so hopefully it will take the same approach this time around. Then again, Ingress didn't have the explosive popularity that Pokémon Go has enjoyed.
Niantic will release more information about these sponsored locations in the near future.


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Direct interaction between Smad3, APC10, CDH1 and HEF1 in proteasomal degradation of HEF1
The Transforming Growth Factor-β (TGF-β) regulates myriad cellular events by signaling through members of the Smad family signal transducers. As a key signal transducer of TGF-β, Smad3 exhibits the property of receptor-activated transcriptional modulator and also the novel ability of regulating the proteasomal degradation of two Smad3 interacting proteins, SnoN and HEF1. It has been shown that Smad3 recruits two types of Ub E3 ligases, Smurf2 and the Anaphase Promoting Complex (APC), to mediate SnoN ubiquitination, thereby enhancing SnoN degradation. The molecular mechanisms underlying Smad3-regulated HEF1 degradation are not well understood. Furthermore, it is not clear how Smad3 recruits the APC complex.
We detected physical interaction between Smad3 and an APC component APC10, as well as the interaction between HEF1 and CDH1, which is the substrate-interacting component within APC. Detailed domain mapping studies revealed distinct subdomains within the MH2 domain of Smad3 for binding to APC10 and HEF1 and suggests the formation of a complex of these four proteins (Smad3, HEF1, APC10 and CDH1). In addition, the protein levels of HEF1 are subjected to the regulation of overexpressed APC10 and CDH1.
Our data suggests that Smad3 may recruit the APC complex via a direct interaction with the APC subunit APC10 to regulate the ubiquitination and degradation of its interactor HEF1, which is recognized as an ubiquitination substrate by the CDH1 subunit of the APC complex.
The Transforming Growth Factor β (TGF-β) superfamily consists of a large group of structurally related polypeptides including various forms of TGF-β, bone morphogenic proteins (BMPs), activins, growth and differentiation factors (GDFs) and the Anti-Mullerian Hormone (AMH, or MIS)
The Smad proteins are the vertebrate homologues of the
HEF1 was first isolated in a screen for human proteins capable of inducing pseudohyphal growth in
In TGF-β induced signaling events mediated by Smad3, it has been shown that Smad3 interacts with both the N-terminus and the C-terminus domains of HEF1 via its MH1 and MH2 domains respectively
The 26S proteasome is a large proteolytic complex present both in the nucleus and the cytosol of eukaryotic cells. Most known proteasome substrate proteins require polyubiquitination to be targeted to the proteasome for degradation. Ubiquitination occurs through the sequential action of three enzymes
The mechanism of Smad3-binding-dependent proteasomal degradation of HEF1 is unknown. For SnoN, it has been shown to involve at least two different mechanisms. First, Smad3 has been shown to recruit the HECT family E3 ligase Smurf2 to regulate SnoN ubiquitination
Here we report the biochemical studies of the interaction between Smad3 and APC10, between HEF1 and CDH1, and the evidence for the functional roles of APC10 and CDH1 in Smad3-regulated HEF1 degradation. These studies suggest a new mechanism of Smad3 in regulating the proteasomal degradation of its interacting proteins via recruiting the cell cycle-linked E3 ligase APC.
Smad3 directly interacts with the Anaphase Promoting Complex (APC) subunit APC10
The yeast two-hybrid system developed in Dr. Roger Brent's laboratory was applied to search for Smad3 interacting proteins, as described previously
First, we tested the ability of APC10 to interact with different Smads in a mammalian overexpression system. HA-tagged Smad proteins (Smad1, Smad2, Smad3, Smad4) were transiently expressed in 293 cells together with T7-tagged APC10. The Smads were immunoprecipitated with anti-HA antibody and the co-precipitated T7-tagged APC10 was detected by Western blot using anti-T7 antibody. T7-APC10 was detected to co-precipitate with Smad2, Smad3, but not with Smad1 or Smad4 (Fig.
Figure 1
Smad3 interacts with APC10 specifically
Smad3 interacts with APC10 specifically. (A) Co-immunoprecipitation of Smad3 and APC10 from mammalian overexpression system. 293 cells were transiently transfected with T7-APC10 and Smad proteins as indicated. The cell lysates were subjected to Western blot analyses directly or immunoprecipitation followed by Western blot as indicated. (B) Smad3 binds to APC10 in GST pull down assays. Flag-tagged Smad constructs were transfected into 293 cells and tested against GST-APC10 purified from E. Coli. Total input Smad protein levels (100 ug) were detected by immunoblotting of the cell lysates with anti-Flag antibody (top panel). Smad proteins bound to the GST-APC10 beads were eluted and analyzed by immunoblotting with anti-Flag antibody (middle panel). As a control, F-Smad proteins were incubated with the GST alone (bottom panel). (*) We noted that GST-APC10 was recognized by the anti-Flag antibody on Western blot. (C) Smad3 binds to HEF1 and APC10 in
Next we tested the interaction between APC10 and the Smad proteins by GST pull-down assay using GST-APC10 expressed and purified from
The interactions were further tested via
Smad3 interacts with APC10 via Smad3 MH2 domain and requires both N- and C-terminus of APC10
To understand how APC10 interacts with Smad3, we carried out deletion analyses to determine the domains of interaction on both Smad3 and APC10. First, GST pull down assays were carried out to test the interaction between purified GST-APC10 and Flag-tagged Smad3 and Flag-tagged Smad3 deletion mutants expressed in 293 cells. The tested Smad3 deletion mutants include Smad3NL, Smad3LC, Smad3 MH1 (Smad3N) and Smad3 MH2 (Smad3C) (Fig.
Figure 2
Domain mapping studies of the interaction between Smad3 and APC10 reveal distinct domains involved in binding
Domain mapping studies of the interaction between Smad3 and APC10 reveal distinct domains involved in binding. (A) The Smad3C (MH2 domain) is necessary and sufficient for binding to APC10. Top panel: a cartoon to illustrate the deletion constructs of Smad3. The APC10 binding activities of each truncated Smad3 as detected by assays in the bottom panels are summarized at the right side of the panel. Bottom left panel: MH2 domain is necessary and sufficient for Smad3 to bind APC10 in GST pull down assay. Flag-tagged full-length or truncated Smad3, as indicated, was transfected into 293 cells and tested against GST-APC10 and GST (negative control). Bottom right panel: MH2 domain is necessary and sufficient for Smad3 to bind APC10 in 293 cells. Different Smad3 truncations tagged with Flag were co-transfected with T7-APC10 into 293 cells. The expression of these proteins was detected by Western Blot using anti-Flag and the interaction between APC10 and Smad3 truncations was detected by immunoprecipitation of T7-APC10 followed by Western blot using anti-Flag. "L.C." represents the antibody light chain. (B) The C-terminal domain of APC10 is necessary for binding to Smad3. Top panel: a cartoon that illustrates the deletion mutants of APC10. The top three deletion constructs are amino-terminal deletion mutants. The number of amino acids deleted in each construct is indicated. For example, the D2 construct lacks the N-terminal 41 amino acids, thus it is also labeled as D41N. Bottom left panel: the six deletion constructs of APC10, each tagged with Flag, were transfected into 293 cells. The expression of these deletion mutants was detected by Western blot using anti-Flag. Stable protein expression was detected only in cells transfected with two C-terminal deletion constructs (D8 and D9). Bottom right panel: the C-terminal 66 amino acids of APC10 are required for Smad3 binding. T7-tagged APC10 and Flag-tagged APC10 D9 were transfected into 293 cells. Cell lysates were subjected to immunoblotting with anti-T7 (lane 1) and anti-Flag (lane 2) antibody. APC10 and APC10 D9 were tested against GST-Smad3 (lanes 3 & 4) and GST alone as a control (lanes 5 & 6).
Further experiments were carried out to determine Smad3 binding domain on APC10. A set of deletion constructs of APC10 (Fig.
Different subdomains within the MH2 domain of Smad3 are involved in binding to APC10 and HEF1
In order to map more precisely the domain on Smad3 necessary for APC10 binding, we tested several Smad3 deletion mutants within the MH2 domain against GST-APC10. In addition, we also tested the binding property of a Smad3/Smad1/Smad3 chimera, which contains N-terminus a.a.1–236 and C-terminus a.a. 277–424 but with part of the MH2 region of Smad3 (a.a. 237–276) replaced with the co-responding region of Smad1 (a.a. 276–317), as previously described
Figure 3
Smad3 MH2 domain contains overlapping but distinct binding sites for HEF1 and APC10
Smad3 MH2 domain contains overlapping but distinct binding sites for HEF1 and APC10. (A) A Cartoon to illustrate the deletion constructs of Smad3 (Smad3d2, d4 and d6) as well as a hybrid protein S3/S1/S3. (B) GST pull-down assay to map APC10 binding site on Smad3C. Flag-tagged Smad3 deletion mutants were transfected into 293 cells and the cell lysates were tested against GST-APC10. Cell lysates (top panel) and proteins bound to the beads (middle and bottom panels) were analyzed by Western blot with anti-Smad1/2/3 from Santa Cruz. The percentage listed at the right side of the listed constructs is derived from dividing the signals (measured by ImageQuant) of bound proteins in the middle panel by the signals of lysate proteins in the corresponding lane of the top panel and then times 100 percent. (C) GST pull-down assay to map HEF1 binding site on Smad3C. Same as in (B), with GST-APC10 replaced by GST-HEF1. (*) endogenous Smad1 and Smad3 recognized by anti-Smad1/2/3 antibody. (D) A cartoon to illustrate the complex of Smad3, HEF1 and APC10 and their potential interaction with the APC ligase core complex.
Since Smad3 MH2 domain has also been shown to bind HEF1
The CDH1 protein in APC complex binds to HEF1
To be ubiquitinated and thus degraded by the proteasome pathway, APC substrates need first to be recognized by either CDC20 or CDH1 which targets the ligase to specific substrates during metaphase-anaphase transition as well as during late anaphase, respectively
Figure 4
CDH1 interacts with HEF1 at the HEF1 C-terminal M2 domain
CDH1 interacts with HEF1 at the HEF1 C-terminal M2 domain. (A) In vitro binding test. In vitro translated 35S labeled CDH1 was incubated with GST-HEF1 or GST alone as a control in modified lysis buffer. 35S labeled CDH1 was separated by electrophoresis and detected by autoradiography. (B) A cartoon that illustrates putative D boxes on HEF1. (C) A cartoon that illustrates the deletion constructs of HEF1 used in (D). (D) Co-immunoprecipitation of HEF1/HEF1 deletions with CDH1 in 293 cells. Full-length HEF1 and T7-tagged HEF1 deletions were co-transfected with myc-tagged CDH1 into 293 cells. Myc-CDH1 was immunoprecipitated with anti-myc antiboby and CDH1 bound HEF1 or HEF1 deletions were detected by immunoblot with either anti-p130Cas antibody (top panel, lanes 1 & 2) or anti-T7 antibody (top panel, lanes 3–5). The expression level of CDH1 was detected with anti-myc antibody (second panel). The amount of HEF1 (third panel) or T7-HEF1 deletions (bottom panel) expressed was detected by anti-p130Cas for HEF1 or anti-T7 antibody. (E) A cartoon that illustrate the putative complex of HEF1, Smad3, APC and CDH1.
CDH1 binds to HEF1 C-terminal M2 domain containing two D boxes
Upon examining HEF1 amino acids sequence, we found five putative D box [RxxLxxxx(N)]. These putative D boxes are located within the SH3 domain (box 1), the SH2 binding sites domain (box 2), after the Ser rich domain (box 3) and within the M2 domain of HEF1 (box 4 & 5) (Fig.
To determine which D box interacts with CDH1, full-length HEF1 as well as T7-tagged HEF1 deletions, as illustrated in a cartoon in Figure
Smad3 interaction with APC10 is regulated by TGF-β type I receptor activation while CDH1 interaction with HEF1 is constitutive
To further test the effect of TGF-β receptor activation on the interaction between Smad3 and APC10 or between CDH1 and HEF1, these proteins were expressed in 293 cells in the presence or absence of a constitutively activated TGF-β type I receptor R4T204D (R4TD). As shown in Figure
Figure 5
TGF-β type I receptor activation enhances Smad3 interaction with APC10 but does not alter CDH1 interaction with HEF1
TGF-β type I receptor activation enhances Smad3 interaction with APC10 but does not alter CDH1 interaction with HEF1. (A) Smad3 interaction with APC10 is positively regulated by the activation of TGF-β type I receptor. Flag-Smad3 and T7-APC10 were co-expressed in the presence or absence of a constitutively active TGF-β type I receptor mutant R4T204D (R4TD). The interaction between Smad3 and APC10 was detected by immunoprecipitation of Smad3 with an anti-Smad3 polyclonal antibody followed by Western blot with anti-T7 (Top panel, lanes 4–6). The immunoprecipitated Flag-Smad3 was detected by anti-Flag antibody (middle panel). The expression of R4TD was detected by anti-TGF-β RI polyclonal antibody from Santa Cruz (Bottom panel). R4TD-p represents a potential cleavage product of R4. (B) HEF1 interaction with CDH1 Is not regulated by TGF-β type I receptor activation. The 293 cells were transiently transfected with myc-CDH1 and HEF1 in the presence or absence of Smad3 and R4TD, as indicated. The interaction between HEF1 and CDH1 was detected by immunoprecipitation with anti-p130Cas followed by Western blot with anti-myc (Top panel, lanes 7–13). The expression of myc-CDH1 and Flag-Smad3 was detected by Western blot by anti-myc and anti-Flag (Top panel, lanes 1–6). The expression levels of HEF1 was detected by anti-HEF1 (middle panel) and the levels of R4TD detected by Western blot with anti-TGF-β RI antibody (bottom panel).
APC10 and CDH1 both regulate Smad3-regulated HEF1 degradation
Since APC10 binds to Smad3, which binds to HEF1 and induces proteasomal degradation of HEF1
Figure 6
Both APC10 and CDH1 can alter the steady state protein levels of HEF1
Both APC10 and CDH1 can alter the steady state protein levels of HEF1 (A) Overexpressed APC10 caused the reduction of the steady-state protein levels of HEF1 and such an activity is compromised in a deletion mutant of APC10 (APC10 D8) that is defective in binding to Smad3. HEF1 was co-transfected with ubiquitin, Flag-Smad3, T7-APC10 or T7-APC10 deletion mutant APC10 D8. The expression level of HEF1 was detected by Western blot using anti-p130Cas antibody, as shown in the top panel; the expression of Smad3, APC10 and APC10D8 were detected by Western blot using anti-Flag and anti-T7, as shown in the bottom panel. (B) CDH1 enhances Smad3-regulated HEF1 degradation. HEF1 was co-expressed in different combinations with myc-CDH1, T7-APC10, Flag-Smad3 and R4T204D (R4TD), a constitutively active type I receptor in 293 cells. The steady state levels of HEF1 were detected by anti-HEF1 antibody (top panel) and the expression levels of Smad3 and APC10 were detected with anti-Flag and anti-T7 antibodies, respectively (second and third panels), while the expression levels of myc-CDH1 were detected by anti-Myc (fourth panel). To detect R4-TD, cell lysates were blotted with anti-TGF-β RI antibody (bottom panel).
To confirm a functional role of CDH1 in proteasomal degradation of HEF1, we also tested the effect of CDH1 on HEF1 protein level, in the presence or absence of Smad3 and APC10, in 293 cells. Like APC10, overexpression of CDH1 alone reduced the protein levels of both forms of HEF1 (Fig.
HEF1 has been implicated in many different signaling pathways such as those mediated by integrin, TCR and BCR where it plays the role of an adaptor protein. Recent studies have shown that HEF1 degradation is regulated by Smad3 via the proteasomal degradation pathway and is further enhanced by TGF-β stimulation
The interaction between APC10 and Smad3 was first observed in the yeast two-hybrid system. This interaction was confirmed in mammalian over-expression system by co-immunoprecipitation, then by
Our previous studies have shown that Smad3 interlocks with HEF1, with the MH1 domain binds to the N-terminal domain of HEF1, while the MH2 domain of Smad3 binds to the C-terminal domain of HEF1
Since APC10 is known to play a role in regulating substrate recognition and ubiquitination by mammalian APC {Hartmut C. et al., Current Biology 13:1459, 2003} the interaction between Smad3, APC10 and HEF1 suggests that HEF1 and Smad3 are potential ubiquitination substrates for APC ligase. Our finding of the direct interaction between HEF1 and the WD40 repeat protein CDH1 further qualifies HEF1 as a substrate for APC, since interaction with CDH1 or CDC20 has been shown to be a prerequisite for APC substrates
While Smad3 can regulate the proteasomal degradation of both HEF1 and SnoN, phosphorylation of Smad3 by the activated type I receptor is required only for SnoN degradation but not for HEF1 degradation
The physiological regulation of the complex formation between Smad3, APC10, HEF1 and CDH1 is likely very dynamic and complex. Since all these observations are made via
In Figure
Figure 7
A cartoon for possible signaling pathways that involve Smad3 interaction with APC10
A cartoon for possible signaling pathways that involve Smad3 interaction with APC10. A cartoon that illustrates possible pathways for Smad3-induced HEF1 degradation involving APC10 and CDH1. Please see text for details.
The studies presented here revealed a physical link between a key signal transducer of TGF-β (Smad3) and a component (APC10) of the cell cycle regulatory E3 ligase APC, and further suggest a molecular mechanism via which such a physical interaction contributes to the regulation of the protein stability of a multi-domain cytoplasmic docking protein HEF1, which is involved in a large network of signaling events. Since Smad3 interacts with many other nuclear and cytoplasmic proteins, this observation may have broad implications for the regulation of these other Smad3 interactors in a similar fashion. While previous studies of the APC complex have been primarily limited to cell cycle control, the observations reported recently extended the role of APC into non-cell cycle events associated with SnoN
Mammalian cell line
293 cells (human kidney cells transformed with adenovirus 5 DNA) were maintained in Dulbecco's modified Eagle medium (DMEM, GIBCO/BRL) supplemented with 10% heat inactivated fetal bovine serum (FBS), 25,000 units of penicillin, 25 mg of streptomycin and 5 mL of 200 mM L-Glutamine at 37°C in the presence of 5% CO2.
Antibodies and reagents
Anti-myc (9E10), anti-Smad1/2/3 (H-2), anti-HEF1 (N-17) and anti-TGF-β RI (V-22) were purchased from Santa Cruz biotechnology. Anti-p130Cas monoclonal antibody was purchased from Transduction Laboratories. Anti-T7 (69522–4) was purchased from Novagen. Anti-Flag was obtained from Sigma and anti-HA was purchased from Roche. Phosphatase inhibitor (P5726) and protease inhibitor cocktail (P8340) were purchased from Sigma. MG132 was dissolved in DMSO and added directly into cell culture medium to a final concentration of 50 μM for 6 hours before harvest.
The pCMV-HEF1 expression vector has been described previously
293 cells were transfected using the standard CaPO4 procedure
Immunoprecipitation and Western blotting
Cells were incubated 30 min on ice in HBS-Lysis buffer (50 mM HEPES, 5 mM EDTA, 50 mM NaCl, 1% TritonX-100 supplemented with protease and phosphatase inhibitors just prior to use). Cell debris was pelleted by spinning in a microcentrifuge at 14,000 g at 4°C for 10 min and supernatant was saved for immunoprecipitation and western blot analysis. For immunoprecipitation, cell lysates were incubated with 2 μg of primary antibody for 2 h at 4°C followed by an additional 2 h-incubation with 40 μL of 50% slurry of protein G-sepharose 4 Fast Flow (Amersham). Beads were then washed once using lysis buffer and 3 times with modified lysis buffer (lysis buffer containing 0.1% of TritonX-100). The precipitated proteins were eluted in 2× SDS loading buffer (100 mM Tris-HCl pH 6.8, 4% SDS, 0.2% bromophenol blue and 20% glycerol) + 10% β-mercaptoethanol, loaded on SDS-PAGE and transferred onto PVDF membrane (Millipore). Membranes were analyzed by Western blotting
GST pull-down assay
GST-APC10, GST-Smad3 and GST-HEF1 were expressed and purified from
- TGF-β: Transforming Growth Factor-β; TCR: T-cell receptor
- BCR: B-cell receptor
- HEF1: Human Enhancer of Filamentation I
- APC: Anaphase-Promoting Complex
- CDC20: Cell division cycle 20
- CDH1: Cdc20 homolog 1
- MG132: Nα-Benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal [Z-Leu-Leu-Leu-H]
- SDS-PAGE: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
- Mv1Lu cell: Mink Lung cell
- MH1, MH2 domain: Mad homology-1, -2 domain
Authors' contributions
CN responsible for carrying out all key experiments, data collection and organizing the data into a thesis format. LM was in charge of the in vivo interaction tests of the interaction between Smad3 and APC10. XL carried out the initial yeast two-hybrid test to isolate APC10 as a Smad3 interactor. Mona Peng contributed to Figure
This research was supported by a Research Scholar Grant award (to Wang T.) from American Cancer Society (RSG-01-184-01-TBE) and Institution fund from Virginia Mason Research Center (C. Nurry and L. Maksumova).
The authors are grateful to technical assistance from L. Feng, J. Farley, A. Reshevsky and A. Elia. The HEF1 deletion constructs are kind gifts from Drs. E. Golemis and S. Law from Fox Chase Cancer Center.
Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta)
Differential expression of the TGF-b isoforms in embryogenesis suggests specific roles in developing and adult tissues
Bone morphogenetic proteins: multifunctional regulators of vertebrate development
Smads and early developmental signaling by the TGFbeta superfamily
TGF-beta signal transduction
TGF beta signals through a heteromeric protein kinase receptor complex
TGF-beta receptors
Activation of signalling by the activin receptor complex
GS domain mutations that constitutively activate T beta R-I, the downstream signaling component in the TGF-beta receptor complex
The immunophilin FKBP12 functions as a common inhibitor of the TGF beta family type I receptors
The TGF beta receptor activation process: an inhibitor- to substrate- binding switch
Crystal structure of the cytoplasmic domain of the type I TGF beta receptor in complex with FKBP12
Signal transduction by the TGF-beta superfamily
Regulation of Smad signalling by protein associations and signalling crosstalk
TGF-beta signaling by Smad proteins
TGF-beta signalling from cell membrane to nucleus through SMAD proteins
A novel ability of Smad3 to regulate proteasomal degradation of a Cas family member HEF1
Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN
The anaphase-promoting complex mediates TGF-beta signaling by targeting SnoN for destruction
Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae
The docking protein HEF1 is an apoptotic mediator at focal adhesion sites
Cell cycle-regulated processing of HEF1 to multiple protein forms differentially targeted to multiple subcellular compartments
Structure and function of Cas-L, a 105-kD Crk-associated substrate- related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes
Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells
T cell receptor-mediated tyrosine phosphorylation of Cas-L, a 105-kDa Crk-associated substrate-related protein, and its association of Crk and C3G
Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase
The ubiquitin system
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction
Direct binding of CDC20 protein family members activates the anaphase- promoting complex in mitosis and G1
The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1
Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex
Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex
Disruption of Apc10/Doc1 in three alleles of oligosyndactylism
Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit
Yeast Hct1 is a regulator of Clb2 cyclin proteolysis
CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis
Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex
SARA, a FYVE domain protein that recruits Smad2 to the TGFbeta receptor
Smad3 allostery links TGF-beta receptor kinase activation to transcriptional control
From mono- to oligo-Smads: the heart of the matter in TGF-beta signal transduction
Ski/Sno and TGF-beta signaling


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|Reviews for Mako Drench
| IndieGoBoom chapter 1 . 11/9/2012
As I was saying, I adore your Nanaki-centred pieces! He doesn't get a lot of love or spotlight in relation to the other characters (he and Barrett, I believe, are especially ignored amongst authors. Cid, too, perhaps) and so its really great to see you harness his character in various ways. Like this piece here felt sort of dark and bitter even, almost as though Nanaki were fearing for himself as he wrote Cloud off as a mere puppet. "He's the messed up one. I'm still Nanaki." That sort of thing, though in the back of his mind he wonders if he's going to be so different. If he could end up the same way.
Great one-shot!
| Guest chapter 1 . 11/9/2012
Honestly, I adore your Nanaki-centred
| Artscapades chapter 1 . 11/6/2009
This is an amazing piece of work. I really can't think of anything else to say I love it.
| Vladimir the Hamster chapter 1 . 8/14/2009
lmao, glow in the dark Cloud. xD I know his eyes do, but I can just imagine his whole body giving off an eerie green light... dom
I thought it was pretty cool how you touched upon Nanaki's experience as an experiment. The scattered thoughts reflect the feeling of helplessness very well, and how it all comes together at the end. Very cool.
| S.Zix chapter 1 . 6/6/2009
I really like this glimpse into Red XI's head. He's probably one of the mentally stronger characters in the game. So much so that people forget that he went through many of the same alterations as Cloud and Sephiroth.
Plus, the weaving of glimpses throughout is intriguing. Though it makes me feel like this is a little rushed. Perhaps if each were lenghtened slightly?
Thanks for the read!
| BananaPeaceMonkeyKarmi chapter 1 . 3/2/2008
I think this is very good.
'I remember it all. That is my curse. He remembers nothing. That is his.'
That's good line. So is:
'I am Nanaki, the son of Seto. I will not go mad. I know who I am.'
Nanaki is what I named him in the game. I didn't like Red XI. No one should have to be an experiment. In that game, or in life.
| Devi11 chapter 1 . 2/12/2008
I like it.
Great to see some Nanaki fic, and even greater to see one that deals with his feelings about having been experimented on by Hojo.
| Mistress 0f Dragons chapter 1 . 1/22/2008
very sad...
... and weird.
| invisiblesplotch chapter 1 . 1/12/2008
Been a while now since i've been around but as usual, it's a pleasure to read your stories, and i really like this one. It's short, but taut and effective. You've really brought out Red's fears and his own burden very effectively. i was actually just playing FFVII and i went through the part where Cloud practically pleaded with Hojo to give him a number and where Red queried Tifa about why she doubted they could go on without Cloud, and you've brought out the whole scenario, the desperation and pathos of it very well.
Red's bits of memories, interspersed between what he's seeing of Cloud, brings out his emotions as well. i think most people overlook the fact that Red was also subjected to Hojo's cruelty as well, and i'm glad to see a fic that touches on it. As a psychological piece, it's great as well. Short and sweet. i also don't really see a lot of really good horror around, and i must say that this is one of the best that i've read, for the skillful way in which you've illustrated Red's deepest fears. It reminds me of another brilliant horror fic i read by Princess Artemis, who is another extremely talented author. It's called "The Ninth Silence". If you like horror, it's definitely worth checking out, i'm pretty sure you won't be disappointed. Great job on your fic, you are truly talented.
(On a lighter note, i still get a chuckle everytime i remember that "splotchy" comment, heh heh)
ANyway, great job once more! )
| Helwi chapter 1 . 10/19/2007
wow, very deep...
It's true that Nanaki's past as an experiment must affects him a lot more than what he is showing in the game...
The situation of Cloud is kinda paradoxal, because in a way, he has been the only successive "Sephiroth's copy" that Hojo "created" and was the one deemed as a faillure...
And Nanaki's sensibility must not help him see the real Cloud among the Jenova's cells and the concentrated memories in the mako.
It should make him doubt of Cloud's true nature indeed.
In a way, he is afraid of Cloud's state, because he can't help but compare it to his own. Like at the time at Gold Saucer when he was scared to be another of the tatooed black claded men or similare to them.
He is still not sure where he stands, but still has the will to keep himself. This is something that make him different of Cloud who was lying to himself about his own identity in order to preserve his sanity.
Nice story :)
| Ginger Ninja chapter 1 . 8/21/2007
Very nice and surreal. It really fit with that part of the story.
| whitealmond chapter 1 . 5/18/2007
Cloud just completely degenerating like that... is so wonderful and horrible at the same time. Red XI's thoughts and the descriptions - I can't praise you highly enough
| Lady Valtaya chapter 1 . 2/16/2007
"...I think he might glow in the dark."
It's almost funny, but kind of sad too, because Red isn't really wrong about saying that. Cloud's eyes do glow in the dark- even when he is recovered from his latest bout with Mako poisoning...
Red's disjointed thoughts make this a little awkward to read, but it is still a good story, because he isn't exactly himself right now- he's reliving his own painful memories and since we know he has some Jenova cells in him too I'm sure that some of the voices in his head are being stirred up from that direction.
Not to say that Jenova has ever had or will ever get control of Nanaki the way she has proven she can do to Cloud, but I don't think that would stop her from talking to her host organisms- whether it is Red or Cloud or even Vincent I think she retains the ability to tease them with nagging self doubt and stuff...
Good work anyway, though I've always thought that Red liked Cloud a little better than he seems to in here...
| Qween of the Damned chapter 1 . 1/30/2007
Its really good. In such a sad, sad way.
| Rosie chapter 1 . 1/1/2007
Wow, this little oneshot is wonderful. It keeps you guessing tp the end who is the narrator. I love your ending including how all these events are affected Red, the most senseible character in the group.
A very intresting topic and well written peice, well done!


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Training & Qualifications
I'm a college student, scored 4s on AP English Language and AP English Literature, and have been accepted into a college on a 3 AM paper with no edits.
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RepairPal is your advocate for car care advice and guidance
Q: Search Results for 93 buick century 3.3 clydiner v6 engine changed the fuel pump on 1993 Buick Century
Over a week ago she just cut off in traffic. She would start but cut off with a loud bang(like a gunshot). I had to tow her home and she set in the driveway for about a week and a half. On 3/19/2012 the fuel pump was replaced and she started up. I was glad if that but didn't try to drive her. I wish that I had, when in park you could give it gas and the engine would rave up. But when in drive or reverse I had to push the gas pedal all the way to the floor and she would not move at all. What is the problem.
| |
could be plugged fuel filter,plugged catalytic converter or trans issues. hope this helps
Changed the fuel filter before the fuel pump was changed. Just got the catalytic converter fixed in October of last year with a whole new exhaust system. Trans mission is what i'm checking now to see if I needed trans mission fluid. Cause it still goes into gear. I have to also check to see if all the wires were hooked back up when ther fuel pump was changed. Could there be something else that is missing if it's not the transmission?
if u just replaced the pump u may need to replace the filter (again) the old pump may have left some sediment in it and that could be the problem
I will have to change that first to make sure that is not the problem. I even checked to codes on the care and I got a 12 repeatedly so that lets me know everything is normal. My father said that it might not be reading the overdrive and I will have to disconnect it to see if that will work but it might not be that. I feel like it could be the filter that wasn't changed when changing the fuel pump. If I continue to have that same problem after the filter is changed then I know that it is something else.
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Rachel Morris (cyclist)
Rachel Morris MBE (born on 25 April 1979) is a British Paralympic sportswoman who has won Paralympic gold medals in both cycling and rowing. She took a gold medal at the 2008 Summer Paralympics as a handcyclist, and eight years later at Rio she won gold in the women's single sculls as a rower. She lost both her legs to Complex regional pain syndrome and cycles, in part, to manage the pain.[1] She was born in Guildford, Surrey.[2]
Morris won two gold medals at the 2007 World Para-Cycling Championships in Bordeaux, France; she won the time trial and road race events in the women's category B races.[3][4] This made her the first ever British hand-cyclist to be crowned a double World Champion.[3]
Morris was named to the team for Great Britain at the 2008 Summer Paralympics, where she competed in the road race and time trial in the HC A/B/C disability category for athletes who use a handcycle.[5][6] She finished sixth in the road race but won the gold medal in the time trial; her time of 20 minutes 57.09 seconds was nearly three minutes faster than her nearest competitor.[5] [7] [8]
At the 2010 UCI Para-cycling Road World Championships held in Baie-Comeau, Canada, Morris won two gold medals.[5] Her first came in the H3 category individual time trial; she won the event by over two minutes in a time of 23 minutes 34.71 seconds.[9] Morris won her second gold medal in the road race, beating silver medallist Sandra Graf by over 80 seconds.[10]
Morris was selected to compete for Great Britain at the 2012 Summer Paralympics, where she was aiming to defend her title in the time trial and also to compete in the road race.[11][12] In July 2012 her participation at the Games was placed in doubt after she was hit by a car during a time trial near her home in Farnham, Surrey.[13][14][15] The incident left her with whiplash and shoulder injuries.[14][15] In addition to her physical injuries Morris' handcycle was damaged in the crash, meaning she had to use her competition bike for training and order a new one for use in the Paralympics.[11] She recovered in time to compete in her first event at the Games on 5 September.[16]
She subsequently took up rowing, in the ASW1x (arms and shoulders women's single sculls) event, came 5th at the 2014 World Rowing Championships in Amsterdam, and won the silver medal at the 2015 World Rowing Championships at Lac d'Aiguebelette in France, thus qualifying for the 2016 Paralympics.
- ^ Williams, Ollie (16 September 2011). "Rachel Morris: Racing against her own body". BBC Sport. Retrieved 13 July 2012.
- ^ "Rachel Morris:Team GB". The Telegraph online. 26 August 2011. Retrieved 13 July 2012.
- ^ a b "World golds for Stone and Morris". BBC Sport. 28 August 2007.
- ^ "GB Cycling Team Rider Biography: Rachel Morris". British Cycling. Retrieved 13 July 2012.
- ^ a b c "Rachel Morris". British Paralympic Association. Retrieved 13 July 2012.
- ^ "Disciplines - Para-Cycling". Canadian Cycling Assoctaion. Retrieved 13 July 2012.
- ^ Aikman, Richard (12 September 2008). "Cyclists trio take GB gold medal haul to 30". The Guardian. London. Retrieved 13 July 2012.
- ^ "More cycling success for Britain". BBC Sport. 12 September 2008. Retrieved 13 July 2012.
- ^ "Sarah Storey wins World Paracycling Road Championships". BBc Sport. 20 August 2010. Retrieved 13 July 2012.
- ^ "Double gold for GB cyclists at World Paracycling Champs". BBC Sport. 22 August 2010. Retrieved 13 July 2012.
- ^ a b "Rachel Morris says injury has shattered her medal hopes". BBC Sport. 12 July 2012. Retrieved 13 July 2012.
- ^ Davies, Gareth A. (26 June 2012). "London 2012 Paralympics: Great Britain para-cycling squad for Games named". The Telegraph. Retrieved 13 July 2012.
- ^ "Paralympic cyclist Rachel Morris in fitness fight after car accident". BBC Sport. 10 July 2012. Retrieved 13 July 2012.
- ^ a b Walker, Peter (10 July 2012). "Paralympic cycling medal hopeful may miss Games after being hit by car". The Guardian. London. Retrieved 13 July 2012.
- ^ a b Davies, Gareth A. (10 July 2010). "London 2012 Paralympics: hand-cyclist Rachel Morris may miss Games after car crash". The Telegraph. Retrieved 13 July 2012.
- ^ Addley, Esther (5 September 2012). "Paralympic handcyclist lines up to race nine weeks after being hit by car". The Guardian. London. Retrieved 5 September 2012.
- Cyclists at the 2008 Summer Paralympics
- Sportspeople from Guildford
- 1979 births
- Living people
- Paralympic cyclists of Great Britain
- Paralympic gold medalists for Great Britain
- Cyclists at the 2012 Summer Paralympics
- English female cyclists
- Paralympic bronze medalists for Great Britain
- Medalists at the 2008 Summer Paralympics
- Medalists at the 2016 Summer Paralympics
- Rowers at the 2016 Summer Paralympics
- English female rowers
- Members of the Order of the British Empire


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RoseAnn V. Shawiak
RoseAnn V. Shawiak (October 6,1950 / New Jersey)
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Aging Childhood Memories
Waving gently like seaweed in the ocean, willow trees sway
slowly in an afternoon breeze.
Silently lifting spirits with their peaceful, serene manner.
Sunlight sliding on and off their expedient leaves.
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Augsburg Digital Archives
We have scanned several collections of our most requested materials and made them available online
- Augsburgian: The student Yearbook was published from 1916 to 2010. All available years are scanned.
- Augsburg Echo: The student newspaper has been published since 1898. All available issues are scanned.
- Alumni Publications: Newspapers and magazines produced by the Alumni Association for Augsburg alumni.
- Graduate Student Theses: Theses for which we have permission to provide online. All theses can be found in paper in the library
- Murphy Square: Augsburg's student-led literary journal of poetry, prose, and the visual arts. Published since 1929 under various names.
- Class photos: Photos of student organizations, campus events and class pictures at Augsburg College and Seminary from the 1880's to the 1960's.
- Campus photos: Historical photos of the campus and buildings.
- Lutheran Free Church: The Lutheran Free Church was active from 1897 until it merged with the American Lutheran Church in 1963. Augsburg houses the archives of the LFC. Some of those archives are scanned.
- Video Archives: Youtube videos of some of the recordings of campus events.
You can find an overview of the history of Augsburg College in two books that we have scanned and provide online:
Chrislock, C. H. (1969). From fjord to freeway: 100 years, Augsburg College. Minneapolis: Augsburg College.
Nelson, R. C., & Wood, D. (2000). The Anderson chronicles: An intimate portrait of Augsburg College, 1963-1997. Minneapolis, Minn: Kirk House Publishers.
The art that you can see in Lindell Library is a complete set of prints called "The Vinland Suite." The book accompanying those prints is reproduced here:
Holtsmark, A., Rosseland, J., & Augsburg College. (2000). The Vinland saga. Oslo: J. Rosseland.
Because the College Archives and Special Collections are staffed on a limited basis, assistance is available by appointment only. For assistance, contact Kristin Anderson, College Archivist:
By e-mail: email@example.com
By telephone: (612) 330-1285
The Archives and Special Collections at Augsburg College include:
- Information relating to the College's history
- Limited information on former faculty, staff and alumni
- The College's permanent art collection
- The Nordic Music Collection