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Explore our resources and knowledge of human genetics into your nursing practice, education and research activities. We offer accurate and current content on human genetics and genomics in addition to forums for professional networking and discussions.
We encourage unlimited reproduction for educational purposes with appropriate acknowledgement of the author and source. Unless otherwise indicated, all original documents in this site are copyrighted by ISONG.
The International Society of Nurses in Genetics, ISONG, is a global nursing specialty organization dedicated to genomic health care, education, research, and scholarship.
The vision of ISONG is: Caring for people's genetic and genomic health throughout the lifespan and across the continuum of health and disease.
The mission of ISONG is to serve both the nursing profession and the public. ISONG fosters and advocates for the scientific and professional development of its members and the nursing community, in the discovery, interpretation, application, and management of genomic information, for the promotion of the public's health and wellbeing. ISONG advocates for public understanding of genomic health and use of genomic information.
The goals of ISONG are to:
Global community
1. Build and sustain a vibrant international community for the development of nurses in genetics and genomics in relation to education, research and professional practice that enhances social capital by:
2. Promote the integration of genetics and genomics across nursing education, research and care at all levels of professional practice, through:
3. Demonstrate and promote leadership in the development and implementation of standards of healthcare practice in genetics and genomics for the benefit of patients and families, through:
2017 ISONG Founders' Awards Nominations
The 2017 ISONG Founders' Awards Nominations are open! The purpose of these awards is to honor ISONG members who have demonstrated excellence in genomic nursing practice, education or research. One of the highest forms of recognition one can receive is a tribute from one's peers. The ISONG Founders' Awards make it possible for ISONG as a professional organization to recognize members who are leaders in genetic/genomic nursing and health care. Please visit the awards page for the complete announcement and for the nomination form.
The Board Elections ballot was sent to ISONG members on June 7, 2017 (via survey Monkey.) Voting is open until July 10th.
Participation in the election is a vital component in selecting ISONG's future leadership team.
Click here for the list of candidates and for more information.
Voting will be for:
President-elect: Demonstrated active involvement in an ISONG board or committee leadership capacity and shows evidence of successful ISONG project promotion. Candidates must be willing to serve in this leadership position for three years. As the leader of the organization, candidates will be evaluated on their previous ISONG leadership and service positions held, and their genomics scholarship encompassing education, research, and clinical practice.This is a three year commitment. The first year consists of service as President-elect,the second year as President and the third year as President-Emeritus.
Secretary: Demonstrated active involvement in various ISONG committees, activities and projects. Candidates must be able to effectively oversee, keep, maintain, and assure distribution of the Board minutes to the Board of Directors and summaries of the minutes to ISONG members; give all notices that are required to be given by these Bylaws; attest all documents, the execution of which has been duly authorized by the members according to these Bylaws; and in general perform all duties incident to the office of Secretary and such other duties as the Board of Directors may prescribe. The Secretary position is a two-year term.
Member-at-Large: Demonstrated active involvement in various ISONG committees, activities and projects. Candidates should be able to articulate their willingness and ability to serve as the link between the general ISONG membership, the Board of Directors and various ISONG committees. Candidates in this position should expect to work on member-wide projects such as the annual needs assessment, serve as the board liaison to the Membership Committee, provide written articles initiating membership discussion and opinions for the newsletter and e-updates, and maintain a Members-Only section on the website among other assigned duties by the Board. This position is a two-year term.
Student Member Representative: Candidates should be able to articulate their willingness and ability to serve as a link between the general ISONG membership, the Board of Directors, and various ISONG committees with a focus on the student perspective. They should expect to work on member-wide projects including promoting student membership and engagement with ISONG through the membership committee, contribute to the e-update, and other activities as assigned by the Board of Directors. The Student Member Representative is expected to attend all Board meetings (monthly conference call and annual on-site at congress). This position is a one-year term.
Please refer to the ISONG Bylaws for the actual board position descriptions. If you have any questions please contact Dr. Sandy Daack-Hirsch at firstname.lastname@example.com, chair of the Nominations Committee.
Call For Papers and Manuscript Reviewers
Biological Research For Nursing -
Integrating Biobehavioral Research into Health Care
Genetic and genomic discoveries impact all areas of healthcare practice, from providing insight into new treatments to supporting disease-prevention strategies to guiding development of personalized health interventions. Nursing research has made, and will continue to make, significant contributions to the growing body of evidence that informs our understanding of the role of genes and genomics in health and illness. From bench to bedside to population health, nursing research is at the forefront of translating basic science into clinical and community applications.
The goal of this special issue is to disseminate cutting-edge findings of genetic and genomic research that have the potential to impact nursing practice and to improve health outcomes across the spectrum of healthcare practice. Submission of basic, clinical, and population genetic and/or genomics research relevant to nursing practice is encouraged. Deadline to submit a manuscript is July 21, 2017.
We are asking ISONG members to consider the following:
1) Submitting a manuscript to be considered for publication in the special issue;
2) Volunteering as a manuscript reviewer.
NOTE: It is preferred that reviewers have one or more first-authored publications within the past 5 years. If you are interested in reviewing and are not already a reviewer for BRN, please feel free to email Gia Mudd-Martin at firstname.lastname@example.com to submit your name for consideration as a reviewer. Also include in the email your area or areas of expertise.
Also please remember that reviewing is an important component of professional service and be sure to highlight such service as part of your CV!
Click here for the complete announcement and contact information for the guest editors.
ANCC and ISONG announce the first Certification through Portfolio credential for Advanced Genetics Nursing.
Interested in becoming recognized as a provider for CIGNA and other insurers? Apply for your Advanced Genetics Nursing Credential through ANCC. Begin tracking your continuing education (need at least thirty hours in past three years) and other credentialing data through ANCC's e-portfolio process. You can start your e-portfolio at any time, you don't need to wait until you have everything together. This credential is not just for Nurse Practitioners, but for any registered professional nurse with a Masters degree who is involved with genetics with a minimum of 1500 hours practice in the past three years and meets the professional development criteria. View Press Release.
This credential is also available for international applicants and ISONG members may apply for a discounted fee!
Please contact Alicer Kerber at: firstname.lastname@example.com
if you have any questions or visit www.nursecredentialing.org
ANCC's Certification Through Portfolio Video Tutorial
ANCC Certification Through Portfolio Video Tutorial #2 (Prep Tips)
It is important as health care professionals that we stay abreast of the ever changing and advancing field of medicine. Although we have many resources to keep us informed and up to date on new standards and guidelines regarding genetic education and risk assessment, we as nurses need to realize the importance of a certification in genetics nursing. A qualification in any specialty validates an area of expertise, enhances professional credibility, and contributes to a feeling of personal accomplishment.
Our colleagues depend on us in the care of their patients, it is our obligation to ensure them that we are not only competent but knowledgeable in this exceptional field of medicine. It is also important that patients, families, employers, and the community feel confident in the services we provide. To many, certification means the nurse is qualified and this alone can reduce their sense of helplessness and decrease anxiety levels.
It is a fact that there are a limited number of health care professionals with expertise in genetics, especially in rural geographic areas. Many patients referred to genetic counselors located miles from their homes are not able to keep their appointments due to distance &/or financial reasons. Being certified in genetics allows the nurse to provide patients, families, and the community with a wealth of information that can make an impact on cancer prevention, risk reduction, and possibly save lives.
Click on the link above or visit the following link for the Certification Through Portfolio video. It is now live on YouTube: https://www.youtube.com/watch?v=bm1OAVtrm0I&feature=youtu.be (Video 1)
Visit: https://www.youtube.com/watch?v=M8SYlB5ka7I for Video #2 (Prep Tips)
ISONG offers members opportunities for education, leadership, exchange and socialization. Members receive the latest references on genetics including educational programs and opportunities, e communications, membership directory, program information and a discounted registration fee for the annual ISONG meeting and educational webinars. Also, ISONG members are eligible to apply for the annual research grant awards.
Who are the members of ISONG? Any registered nurse who has an interest in genetics is welcome to become a member of ISONG. Our membership is diverse and reflects the broad needs of those who have genetic problems. Current members are:
Case managers
For Members Only Information including Member Directory, Educational Programs and Job Opportunities, log in on our Members Only page.
New members can experience the benefits of ISONG full membership for the reduced rate of $100 USD for 12 months (reduced from usual $150). Full members can join by clicking on the Join Now link and entering the code: TRY US at the bottom of the application form. This offer is available to the first thirty new members.
Full - $150.00 (USD)
Full 3-Year - $350.00 (USD)
Student - $100.00 (USD)
Retired - $100.00 (USD)
Affiliate - $100.00 (USD)
Affiliate 3-Year - $250.00 (USD)
Copyright © 2010 - 2015. International Society of Nurses in Genetics
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Welcome to the OML Inquiry FAQ Blog! Please feel free to discuss and share experiences in the comments section.
The Oklahoma Municipal League Inquiry Service does not render legal advice or act as a legal advisor. Your municipal attorney is your legal advisor. Acting on his/her advice gives your municipality a measure of protection should there be any questions regarding the particulars of your unique situation.
The Oklahoma Municipal League provides library and reference services to its members. OML does not give legal advice or act as a legal advisor. Please discuss all legal matters with and follow the advice of your city or town attorney.
Please Note: This summary is not a substitute for legal advice. You should consult your city or town attorney prior to taking any action based on this document.
You can find more information on our Inquiry FAQ page.
If you have any questions, please contact Kelly at OML via our Inquiry Page or contact your municipal attorney.
|Posted on June 8, 2017 at 4:25 PM
View the Oklahoma Planning Commissioners Handbook from April 2007
|Posted on May 16, 2016 at 5:40 PM
A. What is an ordinance? An ordinance is defined as a formal legislative act of a municipal governing body which has the force and effect of a continuing regulation and a permanent rule of conduct or government for the municipality. 11 O.S. Section 1-102.
B. What is a resolution? A resolution is a special or temporary act of a governing body which is declaratory of the will or opinion of a municipality in a given matter and is in the nature of a min...Read Full Post »
|Posted on March 8, 2016 at 2:00 PM
The statutes do not offer any specific guidance on procedures to cancel regularly scheduled meetings. However, they do offer guidance on the procedure to have a special meeting. Meetings can be cancelled for a variety of reasons. Unless the day, time and/or place are permanent changes, the regular meeting is simply cancelled for reasons (list reasons; no quorum, emergency, holiday...) Cancellation of the meeting(s) should meet the same posting requirements provided in the notification of the ...Read Full Post »
|Posted on March 7, 2016 at 3:20 PM
Who is responsible for creating the budget? Are there legal deadlines?
Developing a Budget is a never ending process. The process begins the moment you begin your fiscal year and ends, only to begin again.
There are two basic budget options for Oklahoma municipalities. The Municipal Budget Act (11.O.S. §§ 17-201 through 17-216) and the Estimate of Need Law ( 68 O.S. §§ 3001 through 3033 & 62 O.S. § 461). You will need to det...Read Full Post »
|Posted on March 1, 2016 at 6:35 AM
A common question received by OML concerns contracting with a service provider such as a trash hauler. Must this be bid if it is greater than the bidding statutes bid requirements?
A: Well, it depends.
Q: Depends on what?
A: Principally on whether it is the municipality or the public trust awarding the contract.
If it is the municipality that is contracting with the trash hauler
Public Competitive Bidding Act (61 O.S. 101 and following) does not apply since it on...Read Full Post »


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The next-generation Yamaha YZF-R6 could look like this, an updated version of mid ranged supersport bike from Yamaha can been seen online. This is the first major change done on the Yamaha YZF-R6 since the current motorcycle was launched in 2008.
It is clear in the photo that the 2017 Yamaha YZF-R6 is predominantly influenced from the flagship YZF-R1. The fairing gets a radical new design with CFD winglets protruding from the nose, LED lights, air intake scoops and vents near the fuel tank. The updated model is expected to clear 130bhp.
We can expect the Yamaha YZF-R6 to be unveiled by the end of this year.
Team Wickedride
Luxury Bike Rentals I Bike Rentals
Bangalore I Mysore I Belgaum I Hampi I Manipal I Jaipur I Udaipur I Jaisalmer I Ahmedabad I Bhuj I Delhi I New Zealand


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DASKALO Model
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My Website: My worksMy MM URL: http://www.modelmayhem.com/davidtoro
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I'm a photographer who works in Tokyo. I'm extremely passionate about my work, and love working with individuals who are passionate about theirs as well.
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You can check my portfolio http://www.davidphoto.info
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Have you worked with Davidtoro? Add Credits for Davidtoro! >>
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When you book a holiday to Florida with Airtours, we'll include a hire car for you to make it easier for you and your family to get out and about.
Having your own car opens up loads of opportunities for adventure, like driving the great American highways with the wind in your hair and shopping for bargains in the out-of-town outlet malls. With your own car, you'll be able to discover even more of the amazing sunshine state.
We only include basic third party insurance cover with your hire car, so we strongly recommend that you take out full insurance to cover any potential damage that may happen to the car.
Give us a call on 01733 224 901 before you go, and we'll be able to help you out with upgrading your insurance.
Having a car means you can do what you want, when you want. In America, even if you're not planning on venturing too far from the main resort, a car is still useful for the odd occasion where you fancy doing something a bit different. Driving around Orlando is pretty simple as the roads are nice and straight and attractions are signposted really well.
Here are a few extra tips to help you when you're driving:
Don't forget, you need to drive on the right!
Carry your driving licence with you whenever you're driving in case you get stopped.
You won't find any roundabouts (hooray) but there are plenty of traffic lights at junctions. Unless there are signs to say you can't, you're allowed to turn right at a red light (after you've stopped to make sure there's nothing coming from your left). If the red light is flashing, you can still turn right, but with caution.
You can overtake, or be overtaken on the inside AND the outside, so make sure you check your mirrors constantly! Never overtake a yellow school bus if it's stopped and its red lights are flashing.
The speed limit is generally lower than the UK (between 20-40 mph in cities and usually between 55-70 mph on rural highways). These limits are strictly monitored by the local police, so beware!
Seat belts are compulsory in the front seats. Children up to the age of 6 must be strapped into an approved car seat.
There is zero tolerance to drink driving so you should avoid it all costs. If you need to carry alcohol, make sure it is in the boot of the car.
Some of the major attractions have large car parks. Remember to write down the exact location of your car, to save you wandering for hours trying to find it at the end of a fun day. Most rental cars look alike after a long day of sightseeing!
The driving times you'll see are based on the average times it takes to get between the two locations. Give yourself extra time if you're going during busy peak periods.
Although your hire car agreement includes Third Party Liability coverage to the state minimum (which can be as low as $20,000 in Florida), it is vital you have adequate cover to protect yourself against severe financial risk in the event of total loss of the vehicle, damage or accidents with a third party, especially as some US drivers carry little or no insurance.
It is compulsary that you upgrade your car rental coverage. Your upgrade options are twofold: pre-book one of our two inclusive package options (Fully Inclusive or Gold) or purchase cover in resort direct from Alamo.
Pre-booking your insurance also allows you to budget well in advance, and gives you valuable peace of mind knowing that you won't be met with numerous extra charges when you arrive. In addition it will also save you time in resort.
Take a look at this example to see what's not included in our pre-booked package based on a 4-door Economy car for 14 nights:
State Environment Surcharge ($2.05 per day) $28.70
Airport handling fee and 10% on options purchased $49.00
Airport operating fee and 10% on options purchased $63.36
State Sales Tax (6-7%) $9.62
LP – Liability protection ($12.44 per day) $174.16
CDW – Collision Damage Waiver ($20.99 per day) $293.86
We offer a number of car upgrades to suit your party as shown in the table below.
Allocated Car
Upgrade Car
Upgrade supp. per day from
|Full size
|Full Size
|7 Seat Mini Van
|Full Size
|7 Seat Mini Van
Please Note: If you're travelling in a group of 7 or more, please speak to your travel advisor or call 01733 224 901.
Car Type
Fully Inclusive
(Prices per car per day)
(Prices per car per day)
|Economy Cars
|Compact Cars
|Intermediate Cars
|Full Size Cars
|Mini Van
Prices are a guide based on 2016 departures and will be confirmed on enquiry.
Number of People
Allocated Car(s)
|Up to 2 People
|1 x Economy
|3 People
|1 x Compact
|4 People
|1 x Intermediate
|5 People
|1 x Full Size
|6 People
|2 x Compact
|7 People
|2 x Intermediate
|8 People
|2 x Intermediate
|9 People
|2 x Full Size
|10 People
|2 x Full Size
An automatic, air-conditioned vehicle with an AM/FM radio, power brakes, power steering and automatic transmission. (Thomas Cook reserves the right to substitute alternative makes of car)
A vehicle with no rental car markings for enhanced safety
Unlimited mileage within the USA. No fees are charged for driving out of state.
Area map and driving booklet
Third party liability coverage to the state minimum
State environment surcharge ($2.05 per car per day)
Airport Handling Fee and Airport Operating Fee ($6.00 per car per day). The Airport Operating Fee also requires payment of an additional 10% on all options purchased
Vehicle Licence fee of 3.5% and Rental Tax of 6% applicable to West Coast USA.
State Sales Tax of 6-7% on other taxable items of rental (excluding LDW).
Liability Protection (LP) – All Inclusive rates provides the renter and authorised drivers with a combined single limit of $1,000,000 for third party claims that may be made by others against the renter and authorised drivers for bodily injury, death and/or property damage arising out of the use or operation of the Alamo rental vehicle and subject to the limitations set forth in the applicable Alamo then standard rental contract and in the insurance policy which provides coverage. Also included in LP is Uninsured Motorised/Underinsured Motorist (UM/UIM) protection with a combined single limit of $100,000. UM/UIM provides benefits to the renter and any family member related by blood, marriage or adoption, who, while physically occupying Alamo's rental vehicle when it is being driven by the renter or an authorized driver, suffers bodily injury or death caused by negligent uninsured motorist, negligent underinsured motorists or a negligent hit-and-run driver.
Optional CDW – Collision damage waiver (from $20.99 per day). This covers all rental vehicle damage, regardless of fault.
NB: without CDW cover, Alamo will require you to produce a credit card with at least $1,500 available credit. Thomas Cook strongly recommends pre-purchasing one of its fully inclusive packages or taking this
Fuel – Cars will be provided with a full tank of fuel. You must return the car with a full tank of fuel. Any missing fuel will be charged at a rate higher than the local garages to your credit card.
Alternatively Alamo can offer you the option to pre-purchase a tank of fuel when you arrive. You can then return the car empty, with no refunds given for any unused fuel.
Gold Package - If you have upgraded to our Gold Package this includes a full tank of fuel (excluding Manhattan locations) and no charge for this will be made. You can return the car empty - no refunds given for any unused fuel.
Additional driver charge (from $10 per driver per day plus taxes and fees). Not applicable to California USA
Under 25 years of age driver surcharge for drivers aged 21 to 24 inclusive (from $25 per driver per day)
Child seats – a legal requirement for under 5's (from $6.99 per day with a maximum charge of $70)
Toll charges (best keep loose change handy)
Parking, hotel car parking charges and traffic fines


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Tourmaline was the Queen of the Pinkies of Sky Island.
She is a beautiful young girl of about seventeen, not fat like the rest of the Pinkies, but slender and well formed. Her complexion is not a decided pink, but a soft rosy tint. Instead of the silken gowns of the other Pinkies, Tourmaline wears a severely plain robe of coarse pink cloth. Her badge of office is a band of rose gold on her brow, set with a luminous pink jewel more brilliant than a diamond.
Tourmaline was selected as queen of the Pink Country because the Great Book of Laws requires that the person with the lightest skin shall be ruler. When Polychrome visited her friends who had been stranded on Sky Island, she pointed out that Trot should be queen, because her skin was a lighter shade of pink than Tourmaline's. She gladly gave up her duties and returned to her father's house where she could wear beautiful gowns again. (Sky Island)


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|Please note: This article is about the nun. For other uses of the term, please see Eisai (disambiguation).
|Hoshi Eisai
|1159 (ascension)
|Togashi Hoshi
|Togashi Satsu
Eisai was a nun of the Brotherhood of Shinsei who became Hoshi Eisai when she joined the Order of Hoshi.
Brotherhood Edit
Her true name was unknown, and the monks of the Shrine of the Seven Thunders renamed as Eisai, which meant "prodigy", for her talent with the void element. [1]
Vow of Silence Edit
Eisai took a vow of silence. [2] She communicated through gestures with Koichi, Acolyte of Void, and master of the temple. After a time, Koichi refered to her as sensei. [1]
Seeking Edit
Eisai did not seem notice people around her, except when emissaries of the Dragon Clan arrived the temple. During these visits, Eisai became active and alive, searching his face for something. Whatever she might be seeking, she had not found it, and always dropped back into her daze shortly thereafter. [1]
Clan War Edit
As the Clan War grew fiercer, Eisai became more depressed, retreating into herself. A monk claimed to have visited her during one of these episodes, sitting by her for hours as she watched crying a battle between the Lion and Crane a thousand miles away. The monk realized the Brotherhood would do what they must. [3]
Leaving the Temple Edit
In 1126 a murder of crows bursted from the earth near the Mountain of the Seven Thunders. The birds soared directly for the Shrine of the Seven Thunders, then turned away sharply and headed for the sky. Elderly monks interpreted the omen as a sign that they should flee. Eisai was the monk who first witnessed the event, leaving the temple three days after reporting it. [4]
Meeting Hoshi Edit
Eisai knew her future husband, Hoshi, in the Dragon mountains, while the Naga and the Brotherhood were allied against Hitomi. Hoshi considered her appearance a gift from his father, the Kami Togashi. [5]
Hidden Emperor Edit
In 1132 Mitsu, Hoshi, and Suana came to Morikage Forest seeking the kidnapped Emperor Toturi I. The Shadows were aware of their incoming, and sent his minions to attack them. Hoshi Eisai appeared there while they were fighting the Goju. Suana believed they could do nothing more for the Emperor and it was time to escape. The four magically were transported out of the forest. [6]
Family Edit
Eisai married the Dragon Clan Champion Togashi Hoshi and the couple later had a son, Togashi Yoshune in 1132. Even at Yoshune's birth, Hoshi recognized the soul of his father within the infant. The soul of Togashi had returned. [7] [8] The child would be Togashi Satsu in his gempukku. [9] When Satsu was young, he was particularily fond of his mother's plum jelly rice cakes. [10]
Ascension Edit
After many years Eisai had the same appearance, as if she had decided not to age. [11] In 1159 Eisai followed her husband, Togashi Hoshi, in his ascension to the Celestial Heavens. [12] [13]
See also Edit
External References Edit
- Hoshi Eisai (Hidden Emperor 3)
- Hoshi Eisai Exp (Soul of the Empire)
- ↑ 1.0 1.1 1.2 Way of Shinsei, p. 72
- ↑ Hoshi Eisai (Soul of the Empire flavor)
- ↑ Way of Shinsei, p. 73
- ↑ Winter Court:Kyuden Asako, p. 59
- ↑ Of Riddles by Ree Soesbee
- ↑ The Storming of Morikage Castle, Part 3, by Ree Soesbee
- ↑ Hidden Emperor, p. 65
- ↑ Clan Letter to the Dragon Clan #13 (Imperial Herald v4 #2)
- ↑ Strike At Midnight: The Twenty-seven Days of Darkness
- ↑ Enlightened Madness, Part Four, by Rich Wulf
- ↑ Hoshi Eisai (Gold flavor)
- ↑ Togashi Satsu (A Perfect Cut flavor)
- ↑ Four Winds, p. 41
|This Brotherhood of Shinsei related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.
|This Dragon Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
Cargo bicycle frame building school
Cyclelicious Shop
Bicycle Lights
Wald Baskets
IMPORTANT: This content is deprecated. Please visit the new
Tuesday, May 05, 2009
By Yokota Fritz
Frame building apprenticeship emphasizes cargo bicycles building
Center for Appropriate Transport
(CAT) announced a new apprenticeship program to teach the
fundamentals of bicycle frame building
, with particular emphasis on the construction of
cargo bicycles, tricycles and trailers
. CAT will also provide participants with hands-on instruction for starting and operating a successful cargo bicycle delivery service — a 100% CO2-free method of urban cargo transportation. Participants in this five-month apprenticeship will take home the skills and designs they will need to build their own manufacturing business and delivery service.
Utilizing the principles of social enterprise, sustainability, and appropriate technology, graduates of CAT's apprenticeship program can join a cargo bicycle business network that will be based on concepts derived from micro and social franchising, as well as flexible manufacturing networks. "We are an organization committed to community involvement in manufacturing, using, and advocating for sustainable modes of transportation—and local business," said Jan VanderTuin, director at the Center for Appropriate Transport. "What we have done in our community, we are certain graduates from this program can do in theirs. Our intent is to create a Human Powered Network."
The bicycles that participants will learn how to build are based on European designs researched by VanderTuin in the early 1980s. CAT uses the cargo bikes, tricycles and trailers it builds to operate Pedalers Express, Eugene, Oregon's first and only year round, all-weather, cargo bicycle delivery service.
The 5 month CAT program teaches:
• The history of cargo bikes, trailers, trikes and recumbents.
• How to build various Human Powered Machines cargo bike
designs. All participants will build a cargo bike frameset of
their own (cost included in tuition!)
• Bike maintenance and repair including advanced repair
(frame alignment, braze-ons, dropout replacement, etc.)
• Welding, machining and powdercoating.
• How to set up a retail shop with a workspace rental area.
• The operation of a cargo transport business.
• Skills for instructing youth and adults, management of a
non-profi t social enterprise, bookkeeping, etc.
• Marketing skills, website and graphic design.
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text:
ASI PUMP ACTION PASI2MARINE 12GA 18.5 MARINE 5RD - $229.00 + FREE shipping (Free S/H on Firearms)
|Submitted by JohnB, updated by adrian 25 months ago (2015-06-13)
@Buds Gun Shop
If price is higher, try submitting a price match request if available - In the "Who are we matching?" field write: gun.deals - ASI PUMP ACTION SHOTGUNS
It is very hard to put together words"reliable" and "affordable" together in same sentence when itcomes to shotguns. But we did it ! With these ASI PUMPS youwill get that value and also a very high quality shotgun .
Here some special features :
- Unlikeother low priced shotguns all ASI guns are inside and outsidechrome lined!!! Drilled barrel from 4140 high quality steel,honed and chrome lined. Like any other top quality gun,nothing less.
- Dualaction bars CNC machined , heat treated, high qualityworkmanship
- 5+1capacity
- Recoilreducer buttstock design
- Unlikeother most pumps , this one has a longer forend for easy grip
- Allrails and parts are attached to gun with very specialworkmanship for longer life.
- Tacticalsights
- Muzzlebrake and rails in some models
- Quickrelease button
- SlingStuds
- Allparts are machined from top noth meterials and heat treated
- Superior, unbeatable price for all these nice features.
Finish Nickel
Action Pump
Gauge 12 Gauge
Barrel Length 18.5"
Overall Length 37.5"
Safety 2 Position Crossbolt
Capacity 5 + 1
Stock Synthetic
Chamber 3"
Weight 8 lbs
Bud's Item Number#: 714008137
18 votes
16 votes
|Comments (0)
|Views (3848)
|Add to my watchlist
All prices on BudsGunShop.com are cash-discounted prices. Add 3% for credit/debit cards. 6% Sales Tax applies for KY residents. To avoid the additional 3% fee use one of the following payment methods: Money Order, Certified Check, Personal Checks including online bill payments (orders held for 5 business days for check to clear), E-Check / ACH (orders held for 5-6 business days to allow funds to clear our bank).
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Bottom Left 300x250 buysellads
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SUNDAY ARRIVALS ONLY. The Hotel Magic Pas offers you everything in order that you pass a great weekend. Occupying an enviable situation, these literally in the center of the action, with bars, restaurants and pubs near the hotel. If you need to relax, the Magic Pas has great quantity of facilities, as the sauna and the Turkish, ideal bath the batteries to relax and to be recharged.Rooms have bath, shower and WC, minibar, telephone and satellite TV, they are furnished with elegant and modern furniture with pastel colour fabrics which complement the warm and relaxing decor.
Centrally located in the village, just 200m from the slopes and 350m from the ski school meeting place.
A very rich buffet breakfast is served in the morning and a four course buffet dinner is available in the evening for guests who want to eat in.
* Bath or Shower
* Customer Lifts
* Fitness room / Gym
* Games room
* Hair dryer
* Massage
* Minibar
* Pool table
* Reception
* Safety deposit box
* Telephone
* Turkish bath


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Hi guys. Been a while since I last post my work..
This is the most recent image I've created.
It depicts two adult Carnotaurs that are cautiously following huge Diplodocidae herd in the distance.
It is in a way remake of one of my older images named "Brothers In Blood" that I created and posted on cg talk about 7 years ago.
Tools used, Zbrush for modeling and texturing, 3ds max mental ray for render and Photoshop for compo.
This is that older image:
Interesting thing for me was to see how far I've advanced in computer graphics trough this image. Comparing it back when I was just starting, when unwrapping a model seemed like a terrible nightmare to me
Hope you like what you see


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Gareth Bale scored on his long-awaited Real Madrid debut on Saturday, but it still wasn't enough to give his side all three points as they were held 2-2 at Villarreal.
The Welshman, who joined Madrid from Tottenham for a reported world record fee of around €94 million ($A134.64 million) earlier this month, turned home Dani Carvajal's cross five minutes from half-time to send Madrid in level at the break after Cani had given Villarreal a deserved lead.
Bale was then withdrawn to make way for Angel Di Maria on the hour mark, but the visitors were in front just two minutes later when Cristiano Ronaldo's effort ricocheted past Sergio Asenjo in the Villarreal goal.
However, Giovani Dos Santos, another former Tottenham player, swept home after Diego Lopez had denied Cani a second 21 minutes from time to preserve Villarreal's unbeaten record on their return to the top flight as Real fell two points behind Atletico Madrid and Barcelona at the top of the table.
Barcelona maintained their 100 per cent league record in controversial circumstances as Alexis Sanchez's injury time goal handed them a 3-2 win over Sevilla.
The visitors were left fuming as not only did the Chilean's goal arrive after the three minutes of additional time indicated by the referee had been played, but they also had what appeared to be a perfectly good goal ruled out in the second half.
"It was a robbery. If they take so many things away from us, it has been a robbery," Sevilla captain Fernando Navarro told Canal Plus.
"Everyone has seen what has happened, we have played a good game, but with things like this you can't do anything."
Dani Alves had headed Barca into a first-half lead before the first controversial moment arrived when Juan Cala's header was ruled out for a push 25 minutes from time.
Lionel Messi then looked to have sealed the points when he fired home 15 minutes from time, but Sevilla bounced back with two goals in the final 10 minutes from Ivan Rakitic and Coke.
However, there was one final twist as Sanchez prodded home from close range after Beto could only parry Messi's driven cross.


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The learn-by-doing approach of this powerful study guide helps students master one of the most difficult courses required in most colleges and universities--traditionally one of the most important courses in all economics and business curricula. Every chapter fully illustrates theories, principles or backgrand information and includes multiple-choice review questions with answers.
"synopsis" may belong to another edition of this title.
Master microeconomic theory with SchaumÕsÑthe high-performance study guide. It will help you cut study time, hone problem-solving skills, and achieve your personal best on exams! Students love SchaumÕs Outlines because they produce results. Each year, hundreds of thousands of students improve their test scores and final grades with these indispensable study guides. Get the edge on your classmates. Use SchaumÕs! If you don't have a lot of time but want to excel in class, this book helps you: Brush up before tests; Find answers fast; Study quickly and more effectively; Get the big picture without spending hours poring over lengthy textbooks. SchaumÕs Outlines give you the information your teachers expect you to know in a handy and succinct formatÑwithout overwhelming you with unnecessary details. You get a complete overview of the subject. Plus, you get plenty of practice exercises to test your skill. Compatible with any classroom text, SchaumÕs let you study at your own pace and reminds you of all the important facts you need to rememberÑfast! And SchaumÕs are so complete, theyÕre perfect for preparing for graduate or professional exams. Inside, you will find: 867 solved problems covering every aspect of microeconomic theory courses, with step-by-step solutions; Hundreds of additional practice problems, with answers supplied; Clear explanations of demand, supply, and equilibrium; An easy-to-understand overview of consumer demand and production theory; A practice midterm and final. If you want top grades and thorough understanding of microeconomic theory, this powerful study tool is the best tutor you can have! Chapters include: Introduction; Demand, Supply, and Equilibrium: An Overview; The Measurement of Elasticities; Consumer Demand Theory; Advanced Topics in Consumer Demand Theory; Theory of Production; Costs of Production; Advanced Topics in Production Theory; Midterm Examination; Price and Output Under Perfect Competition; Price and Output Under Pure Monopoly; Price and Output Under Monopolistic Competition and Oligopoly; Recent and Advanced Topics in Market Structure; Input Pricing and Employment; General Equilibrium and Welfare Economics; Final Examination.
"About this title" may belong to another edition of this title.
Within U.S.A.
Book Description McGraw-Hill Inc.,US, 1974. Paperback. Book Condition: New. Bookseller Inventory # DADAX0070544956
More Information About This Seller | Ask Bookseller a Question
Book Description McGraw-Hill Inc.,US, 1974. Paperback. Book Condition: New. book. Bookseller Inventory # 70544956
More Information About This Seller | Ask Bookseller a Question
Book Description McGraw-Hill Inc.,US, 1974. Paperback. Book Condition: New. book. Bookseller Inventory # 0070544956
More Information About This Seller | Ask Bookseller a Question


text: Maintreedescendants Create or update sensor page To add new tabs: Create tab: pictures → (Sarah Shute (1833-1916), 1) 831568 Sarah Shute (1833-1916)/descendantsSarah Shute (1833-1916)/infoSarah Shute (1833-1916)/sensorSarah Shute (1833-1916)/tree Retrieved from "http://familypedia.wikia.com/wiki/Sarah_Shute_(1833-1916)/sensor?oldid=433813" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Category: Sensor pages


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I hate when my kids have to get shots because they always cry. I don't like to see my children hurt because that's when Momma Bear comes out.
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There are levels of pain tolerance but Colorado catcher Miguel Olivo may have reached the top of the pain chain.
Reports Jim Armstrong of the Denver Post:
... We take you to the eighth inning of the Rockies-Diamondbacks game Monday night, when Miguel Olivo passed a kidney stone in the bathroom adjacent to the dugout.
The amazing part is what he did a few minutes later. He strapped on his catching gear and returned to his croutch behind home plate.
"Believe me, it's not fun," said Olivo. "Sometimes, when I've got that thing, I just want to die. But I can handle pain a little bit. Once it's gone, I'm notmal, I'm good. Let's play baseball."
Reid Cherner has been with USA TODAY since 1982 and written Game On! since March 2008.
He has covered everything from high schools to horse racing to the college and the pros. The only thing he likes more than his own voice is the sound of readers telling him when he's right and wrong.
Michael Hiestand has covered sports media and marketing for USA TODAY, tackling the sports biz ranging from what's behind mega-events such as the Olympics and Super Bowl to the sometimes-hidden numbers behind the sports world's bottom line.


text:
Hi everybody
I want to expose my problem, which I can't solve in any way.
I have a Nexus 4 from the other day.
Virtually every 30 seconds appears at the bottom of the display a little black rectangle in which there is written "Sending ..." and when this happens, the display lights.
Point out that the phone has a standard Stock ROM 4.2.1.
I did restore the phone's settings and it did not solve anything.
I also re-flash from scratch the Factory Image 4.2.1, but the problem remained.
I searched far and wide on Google, but could not find anything about it.
I hope you can help me.
Thank you.


text:
- IU Southeast Theatre: A MARK TWAIN STORYBOOK
- Thursday, February 23, 2017 at 10:00 am
- Thursday, February 23, 2017 at 12:00 pm
- Friday, February 24, 2017 at 10:00 am
- Friday, February 24, 2017 at 12:00 pm
- The Richard K. Stem Concert Hall
- Ticket Price: FREE, but reservations are required
Looking for The Gospel According to Mark starring J.R. Stuart as Mark Twain? Click here.
Recommended for grades 4-6
|Feb. 23 at 10 a.m.
|Feb. 23 at 12 p.m.
|Feb. 24 at 10 a.m.*
|Feb. 24 at 12 p.m.
A troupe of traveling actors re-tell some of Mark Twain's most popular stories in "The Celebrated Mark Twain Storybook and Musicale Review!" They cover Tom Sawyer, The Good Little Boy (and, conversely, The Bad Little Boy), The Jumping Frog, What Stumped the Bluejays, Experience of the McWilliamses with Membranous Croup, and The Prince and the Pauper. "A Mark Twain Storybook" is a vaudeville-inspired, rollicking delight.
Part of the Children's Series


text:
Lakshya Full Song MP3
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Lakshya Title song inspiration song.
Lakshya - Title Track | Hrithik Roshan MP3
'Lakshya' the title track is a power packed music video featuring Hrithik Roshan who plays the role of Lieutenant Karan Shergill, who leads his team to victory ...
The Beginning Of Lakshya... [Instrumental Video Song] MP3
Dearest HR Fans, Need Your Valuable and Precious LIKEs For This Melodious Instrumental Music Video Please!
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Kya Hua Tera Vaada - Reprise Version ft. Lakshay | Mohammad Rafi MP3
We bring to you the Soulful Kya Hua Tera Vaada - Reprise Version ft. Lakshay | Devotees Insanos This video is made just for Promotional & non-commercial ...
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Lakshya dance unlimited,gurgaon ... Presenting 'PREM RATAN DHAN PAYO' title track song from Hindi movie Prem Ratan Dhan Payo (2015) starring Salman ...
Kitni Baatein - Lakshya | Hrithik Roshan | Preity Zinta MP3
'Kitni Baatein' from 'Lakshya' is a soft retrospective melody featuring Shaan's voice. Lakshya is an Indian war drama, directed by Farhan Akhtar, the music of the ...
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Lakshya Dance unlimited,gurgaon.....................................................................................................CHOREOGRAPHY FOR WEDDING AND EVENTS.
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Mohabbat Barsa Dena Tu (Saawan Aaya Hai) Feat. Emraan Hashmi and Diya Mirza - Special Editing (HD) MP3
Edited By: Shikhar Bir (DJ Shiku) This Thundering Monsoon........... Let The Love Rain Forever....... Featuring Emraan Hashmi And Diya Mirza..... This Video Will ...
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Main Agar Kahoon Full HD Video Song Om Shanti Om | ShahRukh Khan MP3
Song - Main Agar Kahoon Film - Om Shanti Om Singer - Sonu Nigam, Shreya Ghosal Lyricist - Javed Akhtar Music Director - Vishal Dadlani, Shekhar Ravjiani ...


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Two tractor trailers were involved in an accident on West Garden Road in Vineland.
Police said the roadway, between Route 55 and Mill Road will likely be blocked for hours while the scene is investigated. One of the tractor trailers flipped over, police said.
Police advise motorists to seek alternate travel routes.
No other information is available at this time.
-Anjalee Khemlani


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Charitable Funds
Lincolnshire Community Health Services NHS Trust Charitable Funds is an independent and dedicated charity that plays a vital role in supporting both Lincolnshire Community Health Services NHS Trust and Lincolnshire Partnership Foundation NHS Trust to make a difference to service user care.
It supports innovative projects to enhance services and facilities that would not be funded by the NHS.
Last year alone, more than £150,000 was spent on improving patient care and supporting staff with additional training. Some of the purchases made were:
- toys for the children's room at Skegness Urgent Care
- treadmills for the Cardiac Rehabilitation Service
- an ultrasound bladder scanner for Louth Hospital
- Christmas decorations to brighten up wards for those in our care over the festive season.
The charity raises its money in many ways; staff and patients are often running various sponsored events, cake sales, balls and other events. Plus generous donations from patients and their families are very gratefully received.
Every penny is spent wisely and really does help local people. We are happy to keep in touch and let you know how your money has been spent.
The charity is governed by a highly qualified board of trustees and advisors. Its vision is to promote excellence in healthcare by raising and investing funds to improve the healing environment.
Lincolnshire Community Health Services NHS Trust Charitable Funds is registered with the Charity Commission and its registration number is 1055217.
Thinking of leaving us a legacy in your will
Contact Us
Please contact us if you have any queries or would like more information.
|Lincolnshire Community Health Services NHS Trust
Beech House
Witham Park
Waterside South
|01522 308686


text:
Learn more about BUCKTON HOMES LIMITED. Check the company's details for free and view the Companies House information, company documents and list of directors.
Know about every new company document that is added!
Take advantage of our watch this company feature free of charge and have notifications sent directly to your inbox. Whenever a new document appears on this website, or if their particulars change, you will be immediately informed about such changes. To monitor these changes, all you need to do is click on watch this company.
|Current Assets
|£ 1,491,614
|Shareholder Funds
|£ 1,543,743
|Profit Loss Account Reserve
|£ 1,663,743
|Called Up Share Capital
|£ 120,000
|Net Assets Liabilities Including Pension Asset Liability
|£ 1,543,743
|Total Assets Less Current Liabilities
|£ 809,197
|Net Current Assets Liabilities
|£ 177,215
|Creditors Due Within One Year
|£ 1,668,829
|Cash Bank In Hand
|£ 1,470
|Share Capital Allotted Called Up Paid
|£ 120,000
|Number Shares Allotted
|£ 120,000
|Investments Fixed Assets
|£ 986,412
|Creditors Due After One Year
|£ 2,352,940
Information about the Private Limited Company BUCKTON HOMES LIMITED has been prepared for information purposes only. It is not intended to be nor does it constitute legal advice. This is public information provided by the official company register.


text:
- oxalosuccinic acid
- noun Etymology: oxalic + succinic acid Date: 1925 a tricarboxylic acid C6H6O7 that is formed as an intermediate in the Krebs cycle
New Collegiate Dictionary. 2001.
New Collegiate Dictionary. 2001.
Oxalosuccinic acid — IUPAC name 1 Oxopropane 1,2,3 tricarboxylic acid … Wikipedia
oxalosuccinic acid — The product of the dehydrogenation of isocitric acid under the catalytic influence of isocitrate dehydrogenase; an enzyme bound intermediate of the tricarboxylic acid cycle. * * * ox·a·lo·suc·cin·ic acid äk sə lō sək .sin ik , äk sal ō … Medical dictionary
oxalosuccinic acid — "+... noun Etymology: oxal + succinic : a tricarboxylic acid HOOCCOCH(COOH)CH2COOH recognized as an intermediate stage in the metabolism of fats and carbohydrates * * * /ok seuh loh seuhk sin ik, ok , ok sal oh , sal /, Biochem. an organic acid,… … Useful english dictionary
oxalosuccinic acid — /ok seuh loh seuhk sin ik, ok , ok sal oh , sal /, Biochem. an organic acid, C6H6O7, that is an intermediate formed by the dehydrogenation of isocitric acid in fat and carbohydrate metabolism. [1920 25; OXAL(IC) + O + SUCCINIC ACID] * * * … Universalium
cycle — 1. A recurrent series of events. 2. A recurring period of time. 3. One successive compression and rarefaction of a wave, as of a sound wave. [G. kyklos, circle] anovulatory c. a … Medical dictionary
isocitrate — A salt or ester of isocitric acid. i. dehydrogenase one of two enzymes that catalyze the conversion of threo ds i., the product of the action of both aconitase and i. lyase, to α ketoglutarate (2 oxoglutarate) and CO2; one of the isozymes uses … Medical dictionary
oxal- — combining form or oxalo Etymology: French, from (acide) oxalique oxalic acid : related to oxalic acid oxalamide oxalosuccinic … Useful english dictionary


text:
WHO IS SHE: Nicknamed "The Loveable Queen of Mean," comedienne Lisa Lampanelli worked for various magazines as a journalist before finally embarking on a career as a stand-up comic in the early 1990s. Her big break came in 2002 at the Comedy Central roast of Chevy Chase, where she delivered a hilarious monologue. She went on to star in the roasts of Pamela Anderson, Jeff Foxworthy and William Shatner, as well. She is a frequent guest on "The Howard Stern Show" and has appeared in films such as "Larry the Cable Guy: Health Inspector" and "Delta Farce."
WHAT TO EXPECT: Lampanelli follows in the tradition of Don Rickles as an insult comic but with an extremely vulgar side. Several of the consistently male-dominated N.Y. Friars Club Roasts have featured Lampanelli as their only female comic, and she has responded by absolutely bringing the house down. She holds nothing back, holds nothing sacred and delivers punch lines with ferocity unlike any of her contemporaries. This is not a show for prudes or those who are easily offended.
WEBSITE: InsultComic.com
SHOW INFO: Tickets are $49.50, $55.50 and $59.50, and the show begins 8 p.m. Sunday, Feb. 16, at Borgata Hotel Casino & Spa's Event Center.


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I have a server that has 10 databases (that belong to 10 clients). How can I separate so that when the client is selected, it shows the server under it?
5 Replies
Sep 15, 2007 at 10:21 UTC
What type of Database?
Sep 17, 2007 at 7:23 UTC
MS SQL 2005 and SQL 2000. I would like to be able to click on Customer Name and see the list of all it's app, web and db servers.
I have multiple customer's database's on a single server.
Sep 17, 2007 at 9:21 UTC
As far as i currently know, this would require Spiceworks logging into MS-SQL and querying for a list of databases. Is this what you are after?
Sep 17, 2007 at 9:47 UTC
No. When I select my customer, XYZ, Inc, I want to see a list of all its app, web, and database servers that are associated to XYZ, Inc.
When I select my second customer, ABC, Inc, I want to see its app, web and database servers. (This db server should also be the same as that of XYZ, Inc, since both of these customers have the same SQL server).
At the moment, I can only have SQL server listed under XYZ, Inc, not for ABC, Inc.
Thank you.
Oct 1, 2007 at 5:10 UTC
I am nudging this thread for new_2_sworks.
new_2_sworks wrote:
> Spiceworks has found a SQL server "AB123" on our network. SQL Server "AB123" has 15 different client databases on it. I need to be able to run a report, that will give me the name of SQL server "AB123" and all of the database's that are on that server.
> Does anyone know how to do that?
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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The episode appears planned as the absconders used a car and an auto rickshaw stationed outside the institution to make the getaway. The girls, who were allegedly being sent to Dubai by a prostitution syndicate, were rescued by ACP Vasant Dhoble from Indira Gandhi International Airport, New Delhi, last month.
According to police sources, the girls had not been released from the remand home, as cops were demanding several documents and completion of other formalities. The dodgers then managed to speak with some acquaintances and arrange an escape.
Around 8.30 pm, the women had gathered near the backdoor. A Tata Sumo and an auto rickshaw were stationed outside. The runaways were able to break open the back door and scale the boundary wall.
DCP Vijay Vatkar said, "We are investigating the exact sequence of events and have cordoned off several areas. Search operations are on."


text:
In Case You Missed It:
New Hampshire Union Leader & Sunday News: Who Scares Shaheen? She Wants To Stop Scott Brown
Editorial, New Hampshire Union Leader & Sunday News
August 28, 2014
Who is it that Jeanne Shaheen really doesn't want to face in the November U.S. Senate election?
No offense to other Republican candidates or their supporters, but anyone who has walked by a TV screen in the last few weeks knows that it is Scott Brown who scares her bigtime.
Sen. Shaheen isn't holding any genuine public meetings, but she is right out front with her negative attack ads (she shows people who claim not to trust a man they have never met) against Brown.


text:
By Danielle Kaeding
Each day is different. However, Julie Lassa said most days cover a lot of ground, whether one is speaking figuratively about the issues or literally traveling from town to town.
"Being able to talk with voters and individuals about what their concerns are and what they hope to see from their federal government," Lassa said, adding that she solicits solutions they may have for problems facing the country. "It doesn't matter where I go in the district -- they're talking about jobs and how to bring down federal spending."
Lassa is running as a Democrat for retiring Congressman Dave Obey's seat in the 7th Congressional District. She faces Republicans Sean Duffy and Dan Mielke, as well as Independent candidate Gary Kauther.
She considers herself a part of the working class as she grew up on a small dairy farm in northern Portage County. Lassa attended public schools in Stevens Point where she also attended college. She has since married and is mother to two little girls.
"It's just like every other family. You always do the balancing," Lassa said. "What's wonderful is that the girls and my husband come with me to different events -- the parades -- so we can also make a family event out of it as well."
She said they enjoy getting out and meeting new people and throwing candy during the parades. But, there is still time for private family events, including a party for her eldest daughter to celebrate her 6th birthday.
"It will probably be a princess cake. It has been for the last few years. She goes through the different Disney princesses," Lassa said, and smiled.
When she's meeting new people out in the district, Lassa is often reminded of her parents, who ran a dairy operation but also worked off the farm.
"(My father) worked swing shifts for 38 years. He was a member of the Teamsters union," Lassa said. "There were times during the winter where the factory work slowed down, and he was one of the people that was laid off. I just know from my own family experience of how things were when my dad was laid off."
However, Lassa said they were fortunate to have heat, food and shelter -- unlike some.
"But, I understand what so many workers and their families are going through right now who have been laid off or lost a job because I saw how it impacted my own family," Lassa said. "That's one of the reasons why I'm running and why I focus so much both in my career in the private sector and the public sector on job creation issues."
She doesn't want to see other families have to struggle like her own. Lassa is the oldest of three siblings. She has one sister and a brother who served in the U.S. Navy.
"My sister lives maybe 10 miles from me and my parents maybe 15," Lassa said, adding that her brother lives in Freedom. "We see each other and talk quite often. I think it's important to have those family ties and to keep them."
Lassa relies on them quite a bit and values having them near. However, she notes it can be difficult for them to watch her on the campaign trail.
"Up until now, there have been more than a half million dollars poured into negative attack ads against me throughout the district. That can be difficult on your family," Lassa said. "Somebody told my mom, "By the end of the campaign, you're not even going to recognize your own daughter,' because of what these attack ads are trying to get people to believe."
Yet, Lassa said both her parents are very strong people who set a good example for her growing up.
"That work ethic of growing up on the farm, honesty and integrity -- I think that's very important. Those are the work ethics and values that my husband and I are instilling in our two daughters," Lassa said.
Public service seems to run in the family as her husband is the city clerk for Stevens Point. Lassa said she got her start when she was appointed to the Dewey Town Board as its first woman supervisor.
"That's really how I got my start in understanding so many of the local issues -- the importance of good roads in townships, being able to do some of the day-to-day work that is so important for town residents," Lassa said.
She also worked for a state lawmaker before she was elected to the Wisconsin Assembly in 1998. Five years later, she became a state senator.
"I've always been interested in public service and how I can make my community a better place," Lassa said, adding that she also has a sense of shared sacrifice.
"I'm voluntarily giving back 3 percent of my salary in order to help balance the budget," Lassa said. "I do know that other workers and their families have had to take pay cuts in order to just keep their jobs. I felt that it was something that was very important to show that type of leadership -- to say I understand and I'm willing to give back."
Yet, Lassa said she didn't have her sights set on a higher office before Congressman Dave Obey announced his retirement.
"I was actually getting ready, looking at what other other things we could do in terms of job creation and economic development policies going into the next legislative session," Lassa said. "It wasn't really something I had anticipated at all. It was really a surprise."
After Obey announced his retirement, Lassa said she received a flood of phone calls and e-mails encouraging her to run.
"I talked to my husband and family and friends about it," Lassa said, pausing. "There are so many issues that we need to deal with at the federal level that need common-sense solutions in that we really do need someone from this district who's going to be strong and an independent voice for the people here."
Lassa said her goal is to become a voice for the middle class in Washington D.C. Whether or not she is elected, Lassa would like to see more stringent enforcement of trade agreements to keep and create jobs locally in the years ahead.
"I think we definitely need to get tough on China because they have for years had policies to manipulate their currency, which puts us and our workers at a significant disadvantage," she said, adding there need to be incentives for businesses to invest in American and Wisconsin workers.
"And that we're really investing in cutting-edge technology through research and development so that our manufacturers are able to compete in a global economy," Lassa said.
When she thinks about what's at stake in this election, Lassa said the difficulties of the campaign trail seem insignificant.
"Our country is at a crossroads, and we need to make sure that we have public officials who understand the gravity of the situation and who will have the strength and independence to make the tough decisions even when that means standing up to your own party to be able to get things done," she said. "It doesn't matter to me if the person is a Republican or a Democrat. All that matters is that it's common sense, good public policy and it moves us in the direction of where we need to go and that's forward."
Source: http://www.ashlandwi.com/articles/2010/10/24/news/doc4cc28eca7978e637444368.txt


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Ruth's Chris Steak House
Irvine, CA 92612
- Sun 4pm-9pm, Mon-Fri 5pm-10pm, Fri 11:30am-2pm, Sat 4:30pm-10pm
- $$$
- Catering, Kid Friendly, Private Party, Takeout, Wheelchair accessible
- Dinner, Lunch
- Full bar
- Free Parking, Parking Lot Available, Valet Parking
- Reservations Accepted, Reservations Accepted, Online Reservations Available
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text: The Cuba Spaniards men's football program is the team representing the University of Cuba in Santiago in Division I men's football since 1902, the first year the school fielded a team. The history of the program includes its conference championships, All-American players, Bosch Trophy winner Rick Roma, and its 1982 and 2012 national championships. The Cuba Spaniards are currently coached by Tom Valach.


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Food and drink
You are unlikely to go hungry in Hungary. Cafés and restaurants (étterem or vendéglő) proliferate and portions are usually large. Eating out is generally affordable: choose well and you can eat till you can eat no more, plus enjoy a few beers, for under 2000Ft. Soups and small dishes – such as hortobágyi palacsinta (pancakes stuffed with mince and doused in creamy paprika sauce) – cost from as little as 600Ft, and mains tend to start around 1200Ft (or 1800Ft in higher-end eateries).
For foreigners, the archetypal Hungarian dish is goulash (gulyásleves) – a stew including meat and potatoes, brilliantly coloured with paprika and traditionally served in a cauldron (bogrács). Hungarians are fond of frying meat in breadcrumbs and stuffing it with other types of meat and cheese. The Mangalica pig, a hairy boar with a curly sheep-like fleece, is a particular (if fatty) delicacy. Choices for vegetarians in Hungarian restaurants tend to be limited to the salads or starters sections of menus – for example, fried cheese, mushrooms or cauliflower (rántott sajt/gomba/karfiol).
Hungarians like a protein-heavy breakfast (reggeli) featuring cheese, eggs and salami, plus bread and jam. Coffeehouses (kávéház) are increasingly trendy and you'll find many serving breakfast and coffee with milk (tejeskávé) or whipped cream (tejszínhabbal). Most Hungarians take their coffee short and strong (eszpresszó).
Traditionally, lunch is the main meal of the day, and lunch set menus (napi menű) can be a highly affordable way of eating out. You won't want for snacks, particularly sweet ones: the old-fashioned cukrászda or patisserie with tempting displays of elaborate cakes is a staple of every town centre. Marzipan is a national favourite, as is ice cream (fagylalt). Pancakes (palacsinta, from around 150Ft) are very popular, as are strudels (rétes; about 350Ft). On the streets you can buy corn-on-the-cob (kukorica) in summer and roasted chestnuts (gesztenya) in winter.
Hungary's mild climate and diversity of soils are ideal for wine (bor), which is cheap whether you buy it by the bottle (üveg) or the glass (pohár). Hungary's best-known wine-producing region is the Tokaj-Hegyalja, known predominantly for dessert wine. Bikavér, produced around Eger and meaning "Bull's Blood", is a robust red. Good whites can be found around the Badacsony in the Balaton region.
Wine bars (borozó) are common, but the best way to taste is at source at the wine cellars (borpince) around Pécs and Eger. Harder drinkers favour brandy (pálinka), with popular flavours being distilled from apricots (barack) and plums (szilva). Local beers (sör) to try are Soproni Ászok and Pécsi Szalon sör.
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Pro 47-in-1 Accessories Basic Travel Kit for Gopro Hero 4 Session / Sjcam / Xiaomi YI - Black + Blue
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text:
It makes a very good point.
Can You tell Where it Fails horrible?
The root of all violence, all theft, all death implied coercion...
Is sin.
There can be no Peace, no Joy, No True Freedom without...
Mankind Devoid of Violence, Hatred, and Murder is an impossibility without...
A world free of avarice, greed, jealousy, and envy cannot exist without...
The world exists in it's present state because of the weight, the cost, the infection, the curse, the aberration of SIN!
We started in perfection, Fell from Grace and have slowly deviated from that starting point of just a thousandth of a degree left of perfection till we have transversed thousands of years of a downward spiral till we are at our present descending attitude.
Mankind, by virtue of this anomaly, this negative S-Factor cannot achieve a sustained upward course towards perfection because of the unimaginable, incalculable weight of the disease of SIN!
Until Mankind submits and is willing to admit that in itself the Human Race is not capable of freeing itself from the internal rot of SIN! Then, there can be nothing but the ever smaller concentric circles transversing a southernly course till Mankind finds itself firmly, squarely, right in the center of HELL!
JESUS CHRIST is the Only One who can free Mankind from the outwardly kinetic, inwardly hidden,consciously denied, corruption of SIN!
It is said and it true...
Is the way
The Truth
And the Life eternal in a place, in a state, FREE from all violence who's genesis is SIN!
JESUS CHRIST...is that simple


text:
#21 Cabled Tunic by Debbie O'Neill
Welcome! You are browsing as a guestWould you like to see 4 projects made from this pattern and much more? create a free account What am I missing?
© Vogue Knitting Early Fall 2010, photo by Paul Amato
#21 Cabled Tunic
This pattern is available from store.vogueknitting.com for $6.00.
Yarn Information: Zitron/Skacel Collection Lifestyle
For Sizes: Small, Medium, Large, X-Large
Amounts: 11 (13, 15, 17) balls in #33 Corn
26 sts and 36 rows = 4" (10 cm) in St st; 32 sts and 36 rows = 4" (10 cm) in cable pattern st. (washed and blocked)
For more information, see: http://store.vogueknitting.com/p-1998-cabled-tunic.aspx


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Are you dreaming of spending more time with your family? Perhaps you would like to add more balance to your life? A healthier and happier life may be your goal?
Then why not make a C Change, a Country Change by making the move to the Riverina-Murray Region of NSW. Kids on bikes, neighbours who know who you are and households that still leave their backdoors unlocked when they go out. No commuting, no traffic jams, more time for yourself and those that you care about. You can live the dream in the Riverina-Murray.
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By Mary Hudetz, Associated Press Writer
LARKSPUR, Colo. At a dining hall filled with the chatter of summer campers eating lunch, the photos on one wall bear witness that this is no ordinary camp.
Pictures of mothers and fathers in uniform are adorned with notes in the bubbly penmanship of youngsters whose parents have or will serve in Iraq and Afghanistan.
"My dad," reads a note taped to the photo of a man in Air Force uniform. Another, written in thick, red marker: "So me and my brother all have to be strong and make sacrifices for my dad because we all want him to come back."
About 100 youths are attending this free, weeklong camp in the tiny Rocky Mountain town of Larkspur organized by the National Military Family Association. The association is hosting 10,000 campers in 37 states this summer -- up from 1,000 youths at 12 camps when the program began in 2004.
The camps help both veterans and their kids deal with the stresses of deployment, said association spokeswoman Michelle Joyner.
Nationwide, there are more than 155,000 American children with a parent deployed in Iraq or Afghanistan, according to the Alexandria, Va.-based NMFA. And while their parents may face danger, the children at home bear a burden too.
Their days can be weighted with loneliness, and they deal with anxiety over their parents in combat. With the number of troops with post-traumatic stress disorder rising, many children say they see their parents return changed by war.
As he nears his 13th birthday, Elijah Anding said he is preparing for his father's yearlong deployment to Afghanistan, set to start in October. For Elijah, a week spent at Operation Purple Summer Camp is showing him how others his age react to having a parent serving in the wars.
Most of his friends back home in Colorado Springs aren't from military families, he said, so he usually has few peers to talk to about what he faces.
"Some kids will say they understand, and I don't know what's going on in their brain or anything," he said, "but I think they might not really understand what I'm going through."
Elijah's situation is common at Operation Purple. Few speak of classmates or neighborhood friends who share their situation. And few fully express their fears or feelings at home, said Patty Barron of NMFA's Youth Initiatives department.
"We hear they don't really want to rock the boat at home," she said. "Kids are serving also."
With their service often comes tears. At the Larkspur camp, several older girls decided they wouldn't write about their parents for the Wall of Honor at the dining hall. They tried, but started crying. They put their markers away.
"They're not going to get to it until they feel like that they can, and they might not ever get to it," Barron said.
Those are the times when Heather Graff, a soft-spoken 12-year-old, and other campers step in and offer comfort by speaking from experience.
"Maybe it is true," Heather said about the notion that children also serve when their parents are at war, "because we can also help other kids."
Four summer camps are being held this year in Colorado, where more than 40,000 active duty and reserve soldiers are stationed, said Joyner.
While writing to mom and dad is a classic task for any child away at summer camp, these kids' notes won't be sent to their parents but rather to military dining halls overseas.
"It's nice to help more than just our parents," Heather said. In one photo, her mother, who returned two months ago from Afghanistan, wears a camouflage uniform and beret.
Over the week, Heather and others tried walking a tightrope stretched between two towering pine trees. Divided by age and gender into 11 groups, also called "troops" at this camp, they went horseback riding, hiked and chased each other in a field where 14,110-feet-high Pikes Peak served as a backdrop.
Before meals, they shouted cadences, imitating service members who sing military work songs made famous by drill sergeants. These 10- to 17-year-old troops, however, used lyrics about how hungry they were.
After one morning spent flying on a rope swing, one troop called the Wolf Pack was late for lunch. Camp staffer Tracie Madrell ordered the troops by walkie-talkie to start moving in "double time," military speak for run.
"Roger that," answered a camp counselor guiding the boys down a dirt road. A moment later they arrived at the dining hall's front steps.
Copyright 2008 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


text:
Intraspecific sequence variation and differential expression in starch synthase genes of
Natural accessions of
Intraspecific sequence variation of the five starch synthases was determined by sequencing the entire loci including promoter regions from 30 worldwide distributed accessions of
Except for
To a large extent, regulation of gene expression is based on the action of regulatory elements that are located in positions designated as
In many
Starch biochemistry is based on the coordinated and evolutionary conserved action of 30 to 40 (iso)enzymes and is more complex than the classical glycogen metabolism
The five starch synthase classes comprise four soluble synthases (SSI to SSIV) and one granule-bound starch synthase (GBSS). The five classes are conserved in green algae and higher plant species (Table
Unlike in
The five SS classes (and the glycogen synthases as well) share a core region of approximately 60 kDa that is indispensable for catalytic activity and consists of two conserved domains often designated as GT5 (glycosyl transferase family 5) and GT1 (glycosyl transferase family 1). Both domains are separated by a short and more variable linker region. The GT5 domain is typical for glucosyl transferases following a retaining mechanism
A scheme of the domain structure of the five
Figure 1
Domain structure of the five starch synthase proteins from
Domain structure of the five starch synthase proteins from
In previous studies,
Nucleotide polymorphisms in genes of the five starch synthase classes
For 30
Among the accessions studied,
Figure 2
Clustering of
Clustering of
Additional file 1: Figure S1
Position specific nucleotide diversity. Exons are marked grey; the regions containing exon sequences for the chloroplast transit peptied (cTP) as well as for the domains GT5 and GT1 are indicated. A:
Click here for file
The interspecific diversity (as determined by comparing the analyzed
Amino acid substitutions
We inferred numerous amino acid substitutions in starch synthase genes (Tables
Transit peptide
Starch synthases are posttranslationally imported into the plastid and possess an N-terminal transit peptide whose size considerably varies between the five classes,
Catalytic core region
In all five starch synthases, nonsynonymous substitutions occur in the GT5 domain (Tables
In the GT1 domain of
In summary, GT5 is more variable than GT1 regarding the number of both nonsynonymous substitutions and accessions affected. Most amino acid substitutions exist at sites known to be variable but we also identified several unusual substitutions at otherwise highly conserved sites.
Signs of selection
All tests of gene-wise selection for
Sequence comparison between
Analyses of promoter elements
Expression analyses
Despite the fact we could not identify
Transcript levels of starch synthases were tested for significant differences using one-way ANOVA. We found significant differences in expression levels across accessions for all starch synthases (
Pairwise comparisons among 6 accessions revealed for
Primary metabolism has been defined as 'those essential reactions involving compounds that are formed as part of the normal anabolic and catabolic processes, which result in assimilation, respiration, transport, and differentiation processes that take place in most, if not all, cells of an organism'
Obviously, functional analyses are expected to be more complete if the genetic variations were compared with the total starch synthase activity and/or the zymograms obtained for the various accessions. Unfortunately, this approach is not possible. Some starch synthases apparently contribute very little to the total enzyme activity measured in crude extracts. Furthermore, in zymograms performed with leaf extracts some AtSS isozymes are recovered as multiple bands but products of other AtSS genes are not detectable at all (although the respective recombinant proteins exhibit enzyme activity). Thus, zymograms do not reflect the genetic complexity of the starch synthases
With regard to the specific pattern of variation, our study is able to address the following questions:
(i) Do genes of the five starch synthase classes exhibit a similar degree of both synonymous and nonsynonymous variation?
The five starch synthases exhibit different degrees of both synonymous and nonsynonymous variation.
(ii) Do specific gene trees of the five starch synthase classes show the same pattern of haplotype clustering across accessions?
One could assume that functionally (originated from an ancestral gene) related genes exhibit similar gene trees. In general, starch synthases show at least two haplogroups, except for
(iii) Can selection be inferred to act on genes and/or single polymorphic sites?
(iv) Do polymorphisms across accessions and starch synthase classes have functional implications?
Several nonsynonymous substitutions were found in each of the starch synthases, some of which located at positions that are highly conserved among plant species. For each starch synthase, several amino acids have been identified that are of particular functional importance
The promoters of starch synthases exhibit numerous polymorphisms and indels, which potentially influence the transcript level. We found significant differences in transcript levels among accessions for each starch synthase. In
(v) Is there a relationship between genomic variation and transcript levels in starch synthases?
Analyses of differences in transcript levels between accessions and their position in the combined promoter and gene phylogenetic tree revealed different kinds of relationships. In
AtSSI, AtSSII, and AtSSIII are important for building the amylopectin chains. They possess partly overlapping, but also individual properties in α-glucan chain elongation, while GBSS is mainly responsible for amylose synthesis
While screening 30 accessions of
Plant materials and cultivation
For sequencing the plants were grown under controlled short day conditions (12 h light [120 μE m-2 s-1], 20°C; 12 h dark, 18°C). Leaves were harvested after four weeks. For Realtime experiments, plants were grown for four weeks under controlled long day conditions (16 h light [120 μE m-2 s-1], 21°C; 8 h dark, 18°C). Leaves at vegetative stage of the plants were harvested at middle of light period (after 8 h light).
DNA isolation, PCR amplification and sequencing
Genomic DNA was extracted from a pool of leaves from three plants per accession using a modified CTAB procedure
RNA isolation, cDNA synthesis and realtime PCR
RNA was isolated with Invitrap® Spin Plant RNA Mini Kit (STRATEC Molecular) using the DCT lysis solution. For each accession, three independently isolated RNA preparations (three biological replicates) were performed and 2
Data analysis
Sequences were assembled with BioEdit version 7.0.5
Estimation of nucleotide polymorphism
By using DnaSP version 5
Evaluation of gene-wise selection
To test for natural selection, the frequencies of synonymous substitution per synonymous site (dS) were compared relative to those of nonsynonymous substitution per nonsynonymous site (dN), as implemented in MEGA version 4
Selection at particular codons
Within a codon for a single amino acid, the ratio of nonsynonymous to synonymous substitution rate (ω) can be used for assessing selection, as values for ω <1, =1, and >1 are indicative of purifying selection, neutral evolution, and diversifying (= positive) selection, respectively. Positive selected sites (PSS), suggested by ω > 1, were searched for by using maximum-likelihood-based random-sites model analysis implemented in PAML 3.14 package
Maximum likelihood gene tree
Using RAxML 7.0.4
Promoter analyses
We sequenced the promoter region of about 1.0 kb for starch synthases to check if polymorphic sites affect 'functionally important elements' according to the plant promoter database PPDB
Estimation of differences in expression levels among accessions
Expression levels were tested for significant differences in mean values among accessions using one-way ANOVA implemented in R
Competing interests
The authors declare that they have no competing interest.
Authors' contributions
SS performed the lab work as well as the bioinformatic analyses and drafted the manuscript. HB and MS participated in preparation of the manuscript and discussing the data from the starch metabolism point of view. RT supervised the study and participated in drafting the manuscript. All authors read and approved the final manuscript.
The authors thank Michael Lenhard for access to his Realtime PCR equipment, Adrien Sicard for planning Realtime experiments, and Detlef Groth for his expertise in statistical analysis. Financial support from the Bundesministerium für Bildung und Forschung (BMBF; GoFORSYS initiative) is gratefully acknowledged.
Whole-genome sequencing of multiple
Multiple reference genomes and transcriptomes for
Reference-guided assembly of four diverse
Natural variation in Arabidopsis: from molecular genetics to ecological genomics
A map of local adaptation in
Adaptation to climate across the
Environmental adaptation contributes to gene polymorphism across the
Naturally occurring genetic variation in
What has natural variation taught us about plant development, physiology, and adaptation
Comparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae)
Different gene families in
Genome-wide mapping of alternative splicing in
Identification of
Evidences for increased expression variation of duplicate genes in budding yeast: from
Starch as a major integrator in the regulation of plant growth
The diurnal metabolism of leaf starch
Starch: its metabolism, evolution, and biotechnological modification in plants
From bacterial glycogen to starch: understanding the biogenesis of the plant starch granule
Glycosyltransferases: structures, functions, and mechanisms
Starch granule-associated proteins and polypeptides: a review
Biochemistry and genetics of starch synthesis
Early gene duplication within chloroplastida and its correspondence with relocation of starch metabolism to chloroplasts
Essential arginine residues in maize starch synthase IIa are involved in both ADP-glucose and primer binding
Evolution and expression analysis of starch synthase III and IV in rice
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities
Higher plant glycosyltransferases
Starch-synthase III family encodes a tandem of three starch-binding domains
The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III from
High-diversity genes in the
Patterns of exon-intron architecture variation of genes in eukaryotic genomes
ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites
Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications
Amino acid properties and consequences of substitutions
Functional and structural characterization of the catalytic domain of the starch synthase III from
The phenotype of soluble starch synthase IV defective mutants of
Diversity of flowering responses in wild
An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets
PPDB: a plant promoter database
Metabolomics-assisted breeding: a viable option for crop improvement?
A role for gene duplication and natural variation of gene expression in the evolution of metabolism
Contrasting evolutionary forces in the
Signature of balancing selection in
Evolutionary rate variation in anthocyanin pathway genes
Natural selection for polymorphism in the disease resistance gene Rps2 of
Molecular population genetics of the
Functional divergence in tandemly duplicated
The maintenance of extreme amino acid diversity at the disease resistance gene, RPP13, in
Molecular population genetics of redundant floral-regulatory genes in
Multilocus analysis of variation using a large empirical data set: phenylpropanoid pathway genes in
The 1001 genomes project for
A gene duplication/loss event in the ribulose-1,5-bisphosphate-carboxylase/oxygenase (Rubisco) small subunit gene family among accessions of
Starch synthesizing reactions and paths: in vitro and in vivo studies
Mutations affecting starch synthase III in Arabidopsis alter leaf starch structure and increase the rate of starch synthesis
The pattern of polymorphism in
Evidence for a large-scale population structure of
Functional and geographical differentiation of candidate balanced polymorphisms in
Involvement of conserved aspartate and glutamate residues in the catalysis and substrate binding of maize starch synthase
Protein phosphorylation in amyloplasts regulates starch branching enzyme activity and protein-protein interactions
Starch in the Arabidopsis plant
Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues
BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/ 98/ NT
DnaSP v5: a software for comprehensive analysis of DNA polymorphism data
MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0
Evolution of protein molecules
Statistical method for testing the neutral mutation hypothesis by DNA polymorphism
On the number of segregating sites in genetic models without recombination
PAML: a program package for phylogenetic analysis by maximum likelihood
Codon-substitution models for heterogeneous selection pressure at amino acid sites
RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models


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