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The Marshall Institute today published a new report titled, "A Skeptical Look at the Carbon Tax," by James DeLong of the Convergence Law Institute. Click here to read the report.
In the News
Does Big Green Care about People or Nature?
Ron Arnold, Washington Examiner, 3 May 2013
How Oil Made Working-Class North Dakota Rich
Jordan Weissman, The Atlantic, 2 May 2013
Oil Drilling Leaps, Clean Energy Lags
Jonathan Fahey, AP, 2 May 2013
The High Cost of Zero
Paul Driessen, Washington Times, 2 May 2013
Right Stuff: NASA Scientists Weigh In to Undo Hansen Damage
Robert Bradley, Jr., Master Resource, 1 May 2013
EPA Chief Pretended To Be 'Richard Windsor'
Stephen Dinan, Washington Times, 1 May 2013
Washington Wind Turbine Toppled by 35 mph Gust
Andy Matarrese, Daily Record, 30 April 2013
Government's Bad Bet on Fisker
Charles Lane, Washington Post, 30 April 2013
News You Can Use
Obama's EPA Has Imposed $37.8 Billion in Annual Costs
According to a Heritage Foundation report published this week, EPA has imposed $37.8 billion in annual regulatory costs since President Barack Obama took office. The report, Red Tape Rising, is available here.
Inside the Beltway
Myron Ebell
Senate EPW Schedules Vote for EPA Nominee
The Senate Environment and Public Works Committee has scheduled a vote on the nomination of Gina McCarthy to be Administrator of the Environmental Protection Agency for the morning of Thursday, May 9th. All the Democrats on the committee will vote for McCarthy. Since they hold a ten to eight majority over Republicans, it is certain that the committee will send the nomination to the Senate floor for a confirmation vote.
What is less certain is whether Senator David Vitter (R-La), ranking Republican on the committee, will have the committee's seven other Republicans with him in voting against McCarthy. If he does, then the next question is whether Vitter will lead an effort to block a floor vote.
It takes 60 votes to invoke cloture to end debate and move to a vote. So Vitter needs to round up 41 votes to block McCarthy's confirmation. There are 45 Republicans in the Senate. If Vitter leads the effort against McCarthy, it is likely that he will have two or three Democrats with him. But there are also a number of Republicans who might defect. Several of them don't like McCarthy, but believe that deference should be given to the President's nominees unless they are manifestly unqualified or corrupt.
The argument for blocking McCarthy's confirmation is simply that it is one of the very few shots that Senators will have during the 113th Congress to push back the EPA's ongoing regulatory onslaught against affordable energy. McCarthy, as Assistant Administrator for Air and Radiation for the past four years, has been in charge of writing and promulgating the several Clean Air Act regulations that are designed to close coal-fired power plants. In my view, those Senators who oppose the EPA's agenda should not be voting to promote the point person for implementing that agenda. She also misled both the Congress and the public about the design and impact of two of the most expensive regulations—new fuel economy targets and the Carbon Pollution Standard. My colleagues Marlo Lewis and Anthony Ward explain her duplicity here.
Across the States
William Yeatman
Renewables Repeal Resuscitated in North Carolina
Last Friday, I reported that the North Carolina House Public Utilities and Energy Committee voted down H.B. 298, legislation that would have prevented the state's green energy mandate increasing from 3% to 12% of electricity sales. This week, however, the bill was resuscitated, thanks to the commitment of its sponsor, Rep. Mike Hager (R). Rep. Hager, who chairs the Public Utilities and Energy Committee, told reporters that he would use his prerogative as committee chairman to keep the bill in play indefinitely, and that his goal was to get a floor vote. Hager's bill received another boost this week when its companion legislation was passed out of the Senate Finance Committee by a voice vote.
EPA Shaking Down Contractors?
In January, there was a split in the National Association of Clean Air Agencies (NACAA), an organization that represents air quality officials in state government. Delegations from seventeen states broke off and formed their own organization, the Association of Air Pollution Control Agencies, due primarily to a disagreement over NACAA's support for the Environmental Protection Agency's air quality regulations. Unlike the NACAA, the breakaway groups objected to EPA's regulatory assault, and they sought to create an independent voice.
The new group contracted Battelle, a prominent consulting firm, to administer its launch. Last month, Inside EPA reported that Battelle dissolved the contract with AAPCA as a result of EPA pressure. Sources told Inside EPA that the agency bullied Battelle into dropping the contract, by thretening to block current and potential future contracts with the federal government. This week, the American Tradition Institute sent EPA a Freedom of Information Request Act seeking information about this alleged instance of "gangster government." Also,Texas state officials sent a letter alleging that EPA had "threaten[ed]" Battelle, and demanded to know why.
Around the World
William Yeatman
Another Pointless Climate Confab Concludes
Today marks the conclusion of an intercessional meeting of the United Nations Framework Convention on Climate Change in Bonn, Germany, the purpose of which was to prepare for negotiations at the 19th Conference of the Parties (COP) to the UNFCCC this December in Warsaw, Poland.
According to Bloomberg, almost 190 UNFCCC delegates in Bonn are working towards a deal in 2015 that would establish binding greenhouse gas emissions targets for….2050.This is a pathetic goal, even by the pitiful standards of these climate confabs. The Kyoto Protocol, which was the result of COP-3, created binding targets that its signatories ignored. In 2007, after having spent a decade monitoring the failure of the Kyoto Protocol, climate diplomats at COP-13 in Bali, Indonesia established "aspirational" goals to achieve binding emissions targets for 2020 by COP-15. Two years later, the "Bali Roadmap" dead-ended at COP-15 in Copenhagen, Denmark, where negotiations completely disintegrated.Since the disaster in Denmark, the UNFCCC has been aimless, and this new target is effectively an admission of total failure. A 2050 target is so distant as to be meaningless in practice. It's a goal I could support, and I'm a "denier." Of course, an empty agreement of this sort is the only one that nations of the world would ever submit to, for reasons that I explain here.
The Cooler Heads Digest is the weekly e-mail publication of the Cooler Heads Coalition. For the latest news and commentary, check out the Coalition's website, www.GlobalWarming.org.


text:
Advertisement [CLICK THE BUTTON ON THE RIGHT TO CONTINUE]
Author | Source: Business Line | Wednesday, Apr 05,2006 12:49 PM
Finally, the toy industry in the
country is growing! From stagnating at 5 per cent two
years ago, it has been growing by about 15 per cent
annually, Mr David Daniel Selvaraj, marketing manager,
Funskool India Ltd, told Business Line.
The growth is driven by two factors:
retail expansion and the import of a wide variety of
toys by companies as well as lifestyle stores. Some
department store chains , such as Lifestyle, have even
launched their own branded range of toys.
Imports are growing in quantity as
well as quality. "Five years ago, most of the imports
were junk. But now their quality is going up" and
well-heeled parents are willing to pay, especially if
they are branded, says Mr Selvaraj. Funskool has quite
a few tie-ups to import merchandise based on popular
cartoon/animation series on television channels.
The range of such products include
Beyblades, Disney Princesses and Noddy. It also helps
that these toys are cheaper here than abroad, he
The acceptance of costlier imports
has spurred Funskool to launch pricier toys. Pointing
to a toddler's scooter set in his office, Mr Selvaraj
says it costs Rs 2,399, adding that about five years
ago, the company rarely priced/stocked a toy/game that
cost more than Rs 500.
Most of the imported varieties are
those based on TV series, a host of battery-operated
toys and cars, he said.
However, prices of toys made locally
by Funskool, and of the staple ones, haven't gone up
in the last three years.
Beyblades is the largest selling toy
ever, Mr Selvaraj said. "We sold double of what we
sold of Pokemon," he explained, adding that he expects
the craze to last another year.
Manufactured by Japanese toy major
Takara and imported by Funskool, Beyblades were
introduced in the Indian market in June 2005, soon
after Cartoon Network started airing the series.
Beyblade Championships are a regular
annual feature overseas and were hosted for the first
time in India by Funskool last year in 15 cities.
Season 3 of the Beyblade series called G Revolution
will be aired on Cartoon Network in the next few
With prospects looking up for the toy
industry, Funskool is eyeing a turnover of Rs 50 crore
in 2006-07. While there is no single estimate of the
toy market's size, Funskool estimates it at Rs 450
crore-Rs 500 crore, the unorganised segment accounting
for 60 per cent share.

subset: commoncrawl


text:
Rep. Jim Kasper of Fargo, N.D., may finally be forced to abandon his efforts to legalize Internet poker in North Dakota after the U.S. House of Representatives voted 317 to 93 July 11 in favor of banning Internet gambling in the United States.
Kasper, a longtime supporter of online poker, said he is unsure how the new bill will affect his pro-Internet poker initiative, which he hoped would make North Dakota the first state in the U.S. to license online poker rooms.
The Republican congressman originally introduced the idea during the 2005 Legislature when he proposed a bill and a North Dakota constitutional amendment that would allow the state to license, regulate and tax Internet poker sites, which are currently based in offshore locals such as Gibraltar, Antigua and Costa Rica. His proposals made it through the House but were shot down in the Senate.
He said he is hesitant to give up his support of the initiative because he believes licensing online poker rooms is a great way to generate revenue, which he hopes will be used to fund local property tax cuts. Kasper is also proposing that the state-owned Bank of North Dakota be used to process the financial transactions of each poker room.
Most recently, Kasper was attempting to bring the leaders of the Internet poker industry together to present a unified front to critics, but his attempts failed because of a lack of financial commitment on the part of the online poker industry. Kapser was then faced with the challenge of circulating petitions throughout North Dakota in an effort to bring the issue to a public vote. Unfortunately, this initiative never took off.
Kapser has not given up, however. He has been asked to speak at Poker Summit North America in October and plans to put forth a new online poker proposal to the Legislature in 2007 that will focus on protecting underage and problem gamblers from Internet poker. He said he will consult with industry groups when drafting the new legislation.


text:
TES Quiz: 2 December 2016
1. Which male tennis player holds the record for being ranked number one in the world for the most weeks?
2. Who played God in the 2003 film Bruce Almighty?
3. Seven Pieces For Large Orchestra is an alternative name for which orchestral suite?
4. What was the former name of the Indian city of Chennai?
5. In 2015, which US singer-songwriter released a remake of Taylor Swift's album 1989?
6. Published in 1887, A Study In Scarlet was the first book to feature which fictional detective?
7. The Battle of Britain took place in which year?
8. Which chocolate bar features an outline of the Matterhorn on its packaging?
9. Under which zodiac sign does 2 December fall?
10. Lady Bird was name of the first lady to which US President?


text:
Six-Word Memoirs on Love and Heartbreak
by Writers Famous and Obscure
Larry Smith & Rachel Fershleiser
This book is available for download with iBooks on your Mac or iOS device, and with iTunes on your computer. Books can be read with iBooks on your Mac or iOS device.
Love wounds the heart and soul . . .
From the editors of the New York Times bestseller Not Quite What I Was Planning comes another collection of terse true tales—this time simple sagas exploring the complexities of the human heart. Six-Word Memoirs on Love & Heartbreak contains hundreds of personal stories about the pinnacles and pitfalls of romance. Brilliant in their brevity, these insightful slivers of passion, pain, and connection capture every shade of love and loss—six words at a time.
Customer Reviews
Great Book!
This book is so neat to read and fun. Leaves you pondering and thinking that's me in each section. I'm one of the authors in the book and it's a book you can't put down.
Imitation at Best.
The real, tangible copies of six word memoirs are fun to look at with colorful text and interesting displays. This is black and white, unoriginal, and boring. Good quotes, definitely, but for eight dollars I expected a much higher quality iBook. Don't waste your money on this one, go buy the real thing. (Unless of course they issue and upgrade. Which is doubtful.)
- $4.99
- Category: Biographies & Memoirs
- Published: Oct 06, 2009
- Publisher: HarperCollins e-books
- Seller: HarperCollins
- Print Length: 144 Pages
- Language: English


text:
China's Xiaomi is taking on Apple at its own game, as the world's third-biggest smartphone maker and most valuable tech start-up unveils the flagship Mi Note, its challenger to the smartphone king's iPhone 6 Plus.
Chief Executive Lei Jun introduced the Mi Note in Beijing with a breakdown of the large-screen phone's technical features, with multiple comparisons to Apple's equivalent. At 2299 yuan ($452) for a model with 16 gigabytes of memory, the Mi Note will retail for almost two-thirds less than the iPhone 6 Plus.
"The Mi Note is shorter, thinner and lighter than the iPhone," Lei told his audience of thousands gathered in the north of China's capital.
Lei wore a light-blue shirt, eschewing his previously favoured black top, jeans and sneakers, reminiscent of Apple founder Steve Jobs' trademark black turtleneck and jeans.
"Xiaomi is an innovative start-up company, with a short history," said Lei. The company has been frequently criticised for allegedly copying other tech companies, most notably Apple. "In 10 years we will have tens of thousands of patents."
The Xiaomi Note is absolutely an original design. Glass front and back. Feels great in hand.
pic.twitter.com/IxUfVPSw1U — Ben Thompson (@monkbent)
January 15, 2015
Lei also laid out Xiaomi's strategy to connect its smartphones with Xiaomi-branded home appliances, allowing phone users to remotely control washing machines, air purifiers and surveillance cameras.
"In five to 10 years all devices will become smart devices," he said. "If every smart device and every device at home can be seamlessly connected to the cloud, what could our life be like? It would be beautiful and easy."
Just three years after Xiaomi sold its first smartphone, a $US1.1 billion round of fundraising announced in December valued the firm at $US45 billion.
Xiaomi weathered a decline in overall smartphone sales in China last year to see revenue more than double to 74.3 billion yuan ($US11.99 billion) from 2013.
The company sold more than 61 million handsets in 2014, up 227 per cent from a year earlier. In China, the world's biggest smartphone market, overall sales fell 8 per cent, according to the ministry of industry and information technology.


text:
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News : Xerox Scientists Use Games to Improve Customer Service
Grenoble, France, March 20, 2014 -- Call centers, vitally important to businesses such as airlines and credit card companies, often have agents who say they feel isolated, receiving little feedback on how they are interacting with customers. To help, Xerox researchers have invented software that uses gaming techniques to provide feedback and encourage teamwork.
"Our studies on call centers indicate that embedding game dynamics in the workplace helps agents and their managers set priorities and provides agents with a common challenge that they can collaborate on and talk about," said Xerox researcher Ben Hanrahan.
Social scientists at the Xerox Research Centre Europe (XRCE) in France studied various aspects of how call center agents work to gain a better understanding of challenges. Building upon their findings, researchers created the Xerox Agent Performance Indicator Software (Xerox API).
Over the course of the workday, Xerox API provides agents with continuous feedback on their performance in a fun, interactive way. Using green, yellow and red color codes, agents can see how they are doing in areas such as time spent on a call, number of calls handled and other key performance indicators. Traditional feedback methods involve a manager emailing performance reports to agents once or twice a day.
Agents using the technology show significant improvement on standard performance measures such as the amount of time spent solving a customer problem. The tool also has helped streamline the relationship between managers and their team.
"This innovative approach to providing support and immediate feedback to call center agents has been well received, earning us an Innovation Award from the National Contact Center Association in The Netherlands where it was first deployed, " said Simon Verzijl, Group President Commercial Xerox Services Europe. "The technology encourages collaboration and setting goals along with some fun and healthy competition."
The technology has been deployed to 1,500 agents working in Xerox contact centers in Europe which provide customer care services for 25 Xerox customers.
Xerox plans to expand the use of the tool with planned roll outs in other countries including the United States. With more than 48,000 call center employees supporting clients in 150 locations worldwide, Xerox is a leading provider of customer care solutions, handling more than a million consumer interactions every day via the phone and Web.
Posted by Veronica Silva Cusi, news correspondent
Source: http://news.xerox.com
Related Editorial
- Automation Drives Performance for Xerox Clients
- Xerox in Leaders Quadrant of Gartner's 2016 Customer Management Contact Center BPO Report
- Xerox Announces New Robotic Process Automation Offerings
- Xerox Placed in Leaders Quadrant by Gartner
- Dominican Call Center Workers Slam the Sector
- Xerox Recalling Workers at its Colorado Springs Call Center
More Editorial From Xerox
About Xerox:
Xerox Corporation is a fortune 500 global document management company (founded in 1906) which manufactures and sells a range of color and black-and-white printers, multifunction systems, photo copiers, digital production printing presses, and related consulting services and supplies. Xerox is headquartered in Norwalk, Connecticut (moved from Stamford, Connecticut in October 2007), though its largest population of employees is based in and around Rochester, New York, the area in which the company was founded.
Published: Monday, March 24, 2014


text:
Land sale north of Bali
Land sale north of Bali – The land was located at the first street on the right of GEROGAK, about 8 kilometers west of SERIRIT, so west of LOVINA. Beachfront approximatly 110m. On the beach, on the left side of the land, there is a family PURA of the formel owners, so the land is very well protected, because the whole family is living behind this offered land.
Land sale north of Bali detail
- Land Size : 5.350 m² = 53,50 Are
- Land location : GEROKGAK In the north of Bali
- Land Title : Freehold ( SHM )
- Facilities : Asphalt Access
- View : Beach front
Price : Rp. 95.000.000 / Are ( IDR )
The land is always used to grow vegetables and farm, so it is nice and flat, but because it is not used for years, it is a little overgrown the grass. There is a very good asphalt road to the land. The land is about 200 meters from the main road, so very quite. Electricity is already on the land. At the left side of the land, there is a small river coming from the mountains. At the backside of the land, there are thousands of palm trees, and behind that there is the view on the mountains of the National Park.
Land north of Bali Pictures :
Property code : sale-land-north-bali-001
To get site inspection to the land, please do not hesitate to make an appointment by fill the Form bellow :


text: The Monarch vibrates with a rumble that transfers throughout the entire ring for a mutually pleasurable experience. Features 3 vibration speeds plus pulsation. Its stretchy double-ring design allows for a firm and secure fit, as well as a unique isolation sensation that can help enhance the experience for him. The front ring stretches around the penis while the wider rear ring stretches around the penis and testicles, ensuring the Monarch's flexible wings can gently, yet firmly massage everywhere they touch. Waterproof. Replaceable batteries included.


text:
Reassessment of the
Comparative and evolutionary genomics
The species pan-genome of
This study determined commonly occurring hyper variable hotspots and mobile elements as primary effectors of quantitative gene-scale evolution of species
The genus
Strains of
Virulence of the bacterium is heavily dependent on the virulence gene cluster (VGC, LIPI-1) which promotes cytosolic replication as well as intra- and intercellular movement
A variety of cell wall components are important for the survival of strains of species
To protect from bacteriophage activity, some Archaea and bacteria have developed an adaptive immune system (CRISPR: clustered regularly interspaced short palindromic repeats) based on a variable module of repeats, spacers and protein coding genes (Cas: CRISPR associated)
Small non-coding regulatory RNAs have emerged as a further layer of gene expression regulation in prokaryotes
The pan-genome concept has recently been introduced to explore the diversity of a number of bacterial species and found varying degrees of conservation reflecting differences in habitat, evolutionary pressure and gene pool
This study is the first one to base its evolutionary analyses on a set of 16 completely sequenced genomes of species
Results and discussion
Basic features of strains selected among known serotypes of
In order to analyze the evolution and pan-genomic potential of the species, strains of
The chromosomes compared show a similar size, G+C content, average length of protein coding genes and percentage of protein coding DNA (Table
Pan-genome model predicts a highly conserved species
The pan-genome of 16 chromosomes of
Additional file 1
Species homology matrices. General homology matrices showing the distribution of all coding sequences among 16 strains of species
Click here for a file
Figure 1
Pan-genomic distribution
Pan-genomic distribution. Distribution of CDS based on a homology measure of 60% amino acid identity and 80% coverage. Chromosomes were added 10000 times without replacement in a randomized order and the number of core (mutually conserved) and accessory (found in at least one but not all strains) genes was noted. Since mean and median values for each step showed only little variation the mean numbers of gene classes were plotted. In order to predict a possible future pan-genomic distribution for this species we performed a power law fitting. A) Pan-genomic CDS after each consecutive addition of a strain, B) mutually conserved CDS, C) conservation of CDS and homology clusters.
Other studies relying on the hybridization of eight lineage III strains on a microarray based on 20 strains (two complete, 18 draft chromosomes) found a closed species pan-genome
Summarily, our research shows a conserved species, which tolerates low levels of horizontal gene transfer.
Hyper variable hotspots contain one fourth of the accessory genes and permitted the insertion of major pathogenicity determinants
The accessory gene content of compared strains is not scattered evenly across the chromosomes, but accumulates in nine defined chromosomal regions supporting previous observations considering the clustered distribution of strain-specific genes
Additional file 2
Insertional hotspot ranges. Hotspots showing at least three separate insertions denoted by locustag ranges.
Click here for a file
Additional file 3
Comparative genomic GECO figures of hyper variable hotspots. Comparative GECO depictions of insertional hotspots highlighting extensive mosaicism.
Click here for file
Only a small number of genes could be identified inside hotspot loci which exhibit an obvious adaptive value for the host genome, including the previously described pathogenicity determinants
These hyper variable hotspots have previously been suggested to be the result of a founder effect resulting from a primary insertion that did not reduce the fitness of the respective strain, which now offers a larger target for neutral insertions, thus increasing their likelihood
Chromosomal mobile genetic elements are major sources of diversity – prophages, transposons and genetic islands
In order to find large insertions in the chromosomes of the respective strains we plotted all coding sequences, which were not conserved in all strains, resulting in the identification of between one and five mobile genetic elements (MGE) such as prophages, transposons, insertion sequences and genomic islands per chromosome (Figure
Additional file 4
Mobile genetic elements. Distribution of mobile genetic elements ordered by relative position in the chromosome of
Click here for a file
Figure 2
Insertions between syntenic core genes
Insertions between syntenic core genes. Bar chart of CDS inserted between syntenic core-CDS existing in all strains depicted relative to reference strain 1/2a EGD-e. The oriC inversion of strains 08–5923 and 08–5578 was removed for this analysis. Mobile genetic elements (MGE) are classified as prophage (red triangle), transposon/IS element (blue square), genetic island (green circle). The MGEs were numbered according to their relative position in strain 1/2a EGD-e. Putative anchor genes in the chromosome (ex.: tRNA, comK) are included in square brackets. If different elements inserted at the same chromosomal locus, the strains involved are denoted in round brackets. Multiple designations per element are delimited by a colon. If an element was not described yet, the genus bearing the highest overall nucleotide similarity to the respective region was included instead (e.g.
Among these are 8 different prophages which are typically inserted by site-specific recombination into chromosomal loci adjacent to tRNA genes as previously observed
Three putative transposons were identified in the strains studied. Two of them are located between homologues of genes
Additional file 5
Comparative genomic GECO figures of transposons ICELm1 and TN554. Comparative GECO depiction using a homology measure of 60% amino acid identity and 80% coverage. Displays content and conservation of two transposons.
Click here for file
Additional file 6
Comparative genomic GECO figures of IS3 elements. Comparative GECO depiction using a homology measure of 60% amino acid identity and 80% coverage. Displays duplication of IS3-like transposon.
Click here for file
Another type of MGE is designated genomic island and denotes a module of genes inserted by horizontal gene transfer which frequently encodes fitness conferring genes and typically contains at least one integrase gene employed for mobility. One of these was called Listeria genomic island 1 (LGI1) and putatively introduced by serine recombinases into 1/2a 08–5923 and 08–5578
In summary, nearly one third of the accessory genes of the species have been introduced by identifiable MGEs, representing a large proportion of gene-scale diversity
CRISPR/Cas systems represent supplementary bacteriophage defense mechanisms for the species
Chromosomes of
Additional file 7
Comparative genomic GECO figure of CRISPR/Cas loci. Comparative GECO depictions of three CRISPR/Cas loci using a minimum CDS homology measure of 60% amino acid identity and 80% coverage.Cas genes and spacer/repeat arrays are framed. Locus 1 displayed no associated Cas genes. Locus 3 includes a
Click here for file
Additional file 8
CRISPR/Cas loci. Homology matrices and positions of CRISPR/Cas genes and associated arrays of three loci. Spacers were additionally mapped versus the NCBI nt database to identify possible target sequences.
Click here for a file
All strains bear a putative remnant of a CRISPR-system at ca. 0.5 Mb in strain 1/2a EGD-e which is not associated with any
Locus 2 is located ca. 10kb adjacent to locus 1 and resembles the
Locus 3 is inserted into homologues of a lipoprotein gene (
All identifiable spacers (81/276) are directed versus known listeriaphages or related composite prophages. We also encountered multiple different spacers homologous to sequences of the same phage in the same array, as well as identical duplications of one spacer. It is tempting to speculate that inclusion of redundant spacer sequences increases the likelihood of a successful defense against the respective bacteriophage (ex.: A118, A500, B025). We never observed identical spacers to be present in multiple arrays, indicating a clear separation of all loci. No spacer was found to target chromosomal or plasmid sequences of species
In conclusion, we propose that an ancestor of genus
Phylogenies compared – relationships between lineages, serogroups, serotypes and strains according to genomic and genetic content
This analysis used the complete genomic sequences of 19 strains of genus
In order to enable phylogenetic clustering we created a well-supported (bootstrap >80%) core-genome tree based on an alignment of all concatenated core genes (2018) of 19 strains using Mugsy
Figure 3
Phylogenomic and -genetic trees
Phylogenomic and -genetic trees. (A) Neighbor joining tree based on an alignment of 2018 mutually conserved core genes (amino acid identity >60%, coverage >80%) of 19 strains of genus
We additionally clustered all strains based on the accessory gene content (presence/absence of 2953 genes) to identify the impact of gene-scale indels, which includes most horizontal gene transfer events
If only gene gain and loss are taken into account, lineages of
The opposite is apparent when considering phylogenomic groups, which were found to be closely related in the core-genome tree but to a much lesser degree considering gene content, implying a number of young indels. Interestingly, phylogenomic groups are located at the end of shorter common branches in the gene content tree, which is due to a small number of exclusively conserved genes (PG1: 28, PG2: 20, PG3: 22, primarily hypothetical and truncated genes) (Additional file
We identified three topological changes between core-genome and gene content tree hinting at shared indels that run contrary to the phylogenomic signal of core-genome SNPs. Removal of genes related to mobile genetic elements (34% of accessory genes) from the gene content matrix resulted in a topology very similar to the core-genome tree. Thus, large-scale insertions, which resulted mainly from bacteriophage integration, run contrary to the "true" phylogenetic signal by inserting many genes in one event as well as by putative parallel insertions into different strains. The only remaining difference was observed considering a common branch for strains of lineage III and apathogenic species, highlighting small-scale indels as causative force. This supports a previous hypothesis suggesting lineage III as a possible deleterious intermediate state between lineages I/II and apathogenic species
Interestingly, the majority of accessory genes of species
In summary, tree topologies based on a core-genome alignment and gene content were found to be highly similar despite the obfuscating influence of mobile genetic elements. Other studies on
Frequent loss and disruption of known virulence-associated genes may explain observed phenotypic attenuations
About one third of the genes which displayed compelling evidence for involvement in the infectious process were found to be absent or to code for a truncated protein in at least one of the strains studied, putatively impacting the disease phenotype (Additional file
Additional file 9
Known virulence genes. Homology matrix of known virulence genes.
Click here for a file
Additional file 10
Click here for a file
Additional file 11
Growth curves BHI. Growth of
Click here for file
Figure 4
In order to correlate phenotypes with genomic differences we performed detailed analyses of virulence-associated genes that allow us to present hypotheses on the evolutionary descent of these changes (Additional file
Additional file 12
Detailed analyses of reductive evolution of virulence-associated genes. In-depth information about previously described virulence and pathogenicity indicated genes that are absent or truncated in one of the compared strains.
Click here for file
In conclusion, strains of
Distribution of surface-associated genes displays conserved lineage-backbones with strain-specific adaptations
A detailed examination was undertaken to spot relevant patterns of presence or absence of surface-associated genes mediating interaction with the environment and the infected host, and to invoke evolutionary explanations (Additional file
Additional file 13
Plot of Surface-associated CDS. Bar plot depicting the distribution of all surface-associated protein coding genes among studied strains.
Click here for file
Additional file 14
Distribution of surface-associated genes displays conserved lineage-backbones with strain-specific adaptations. Detailed analysis of presence and absence of surface-associated genes.
Click here for file
To conclude, genes bearing P60 or LysM domains showed little variation among the strains studied (Additional file
Additional file 15
Surface-associated CDS. Homology matrices of genes containing a surface-associated domain (NLPC/p60, LysM, GW, LRR, LPxTG, Lipobox, signal peptide).
Click here for a file
Internalins are involved in cell adhesion and invasion of host cells and contain a leucine-rich repeat (LRR) domain indicated in protein-protein interaction (Additional file
Additional file 16
Internalins. Homology matrix of genes containing a leucine rich repeat domain and an optional signal peptide.
Click here for a file
Additional file 17
Putative transposition of lipoproteins
Click here for file
Taken together, we found that most surface-associated genes are either mutually conserved or were likely present in an early ancestor of a lineage, implying a fixed core-functionality that is rarely complemented by strain-specific additions confirming previous observations
Ancestral genes of serotypes, serogroups and lineages reveal new marker and virulence-associated genes while strain-specific genes rarely represent an obvious extension of functionality
In order to identify conserved ancestral genes which may be important for the differentiation of lineages, we collected genes that were found in all strains of a lineage (>60% amino acid identity, >80% coverage) and absent in all strains of other lineages (Additional file
Additional file 18
Lineage-specific CDS. Homology matrix of coding genes specifically present in one lineage.
Click here for a file
The adaptation of strains of lineage III furthermore included loss of genes implicated in food preservation measures, pathogenicity, or virulence as previously described
Additional file 19
Lineage I/II exclusive CDS. Homology matrix of genes conserved in 13/14 strains of lineages I and II, while being absent from both strains of lineage III.
Click here for a file
We also tried to identify exclusive indels for serogroups or –types that are represented by at least two strains in this analysis in order to uncover ancestral sequences (Additional file
Additional file 20
Serogroup and –type ancestral indels. Homology matrix of CDS found to be commonly present or absent (ancestral indel) for either one or multiple serogroups or -types.
Click here for a file
In order to assess the impact of recent adaptations, strain-specific genes were examined (Additional file
Additional file 21
Strain-specific CDS. Homology matrix of coding genes specifically present in one strain.
Click here for a file
Small non-coding RNA candidates of
Previous transcriptomic analyses uncovered 210 regulatory sRNA candidates expressed in
Additional file 22
Small non-coding regulatory RNAs. Homology matrix of sRNA candidates.
Click here for a file
Only 43 of these were found to be accessory sRNAs, defined as being absent from at least one compared strain, including 20 sRNAs that are only present in a subset of strains of lineage II. Approximately half of those differentially distributed sRNAs, that were previously suggested to be involved in virulence or pathogenicity by growth attenuation of deletion mutants in mice (
We found only
Interestingly, strain 3a SLCC7179 shows a fragmented homologue of
In summary, evolution of small non-coding RNAs represents an ongoing process in species
LisDB – a comparative genomics server for the
A large part of the analysis presented in this study is based on the GECO comparative genomics software
The presence or absence of genes among all serotypes of species
The 11 isolates to be sequenced were selected to achieve full coverage of serotypes of species
Automatic annotation was performed by GenDB, which includes steps for the identification of protein coding sequences (CDS), rRNA and tRNA genes as well as similarity searches against major gene and protein databases
Comparative analyses
Homologous coding sequences were identified by BLASTCLUST
Pan-genome analysis
The pan-genome size of
Identification of large insertions
The colinearity of chromosomes of
CRISPR/Cas analysis
Spacer/repeat-arrays were identified with PILER-CR 1.02 and CRT 1.1 using standard parameters with the exception of maximum repeat length, which was increased to 40
A phylogenetic core-genome tree was created based on mutually conserved core CDS of all compared strains including out-group strains
In order to identify the impact of indels on phylogeny we built a second tree based on the presence and absence of 2953 accessory genes using GeneContent
Identification of surface-associated genes and putative internalins
Surface-associated genes were identified based on sequence similarity to known motifs (P60, LysM, GW, LRR, LPXTG, lipo) using various Hidden Markov Models (HMM) and SignalP as implemented by Augur
All CDS containing a leucine rich repeat (LRR) domain were assumed to be putative internalins and checked for the presence of a signal peptide. False positives and negatives as revealed by synteny analysis were corrected manually and the homology cutoff was reduced to 50% identity and 40% coverage if necessary. Apprehension of internalin-types based on predicted internalins from a previous study
Measurement of bacterial growth
Bacterial cultures were grown over night at 37°C in brain heart infusion broth (BHI) and diluted 1:200 the next day for fresh cultures. Automated measuring at 37°C was performed using the Infinite 200 plate reader (Tecan) in 96-well plates with 150 μl volume/well.
In order to assess the degree of pathogenicity of the 16 strains studied, the insect model
Analyses of sRNAs
Multiple studies have previously determined small non-coding RNA candidates of species
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
CK carried out bioinformatic tasks related to the sequencing process, performed annotation and comparative analyses and drafted the manuscript. AB participated in the annotation and bioinformatic analyses and assisted in drafting the manuscript. MM performed phenotypic experiments. AS performed the genomic sequencing. AG participated in the design of the study. RD participated in the design of the study. SB assisted in drafting the manuscript. TH conceived of the study, participated in its design and coordination and assisted in drafting the manuscript. TC participated in the design of the study and assisted in drafting the manuscript. All authors read and approved the final manuscript.
This project was funded by the German Federal Ministry of Education and Research through ERA-NET program grants sncRNAomics and SPATELIS, and the LOEWE program of the state Hessia collaborative research project Insect Biotechnology to T. H. and T. C., as well as the German Centre for Infection Research, Justus-Liebig University Giessen.
We thank Sonja Voget, who performed the majority of the sequencing experiments that this project is based on and Anita Hoeland, who constructed data visualizations. We would also like to acknowledge Mark Achtman and Jana Haase for retrieving information about isolates stored in the
Evolutionary history of the genus
Listeriosis: a resurgent foodborne infection
Food-related illness and death in the United States
A new perspective on
New aspects regarding evolution and virulence of
Lineage specific recombination rates and microevolution in
Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of
Comparative analysis of plasmids in the genus
Investigation of specific substitutions in virulence genes characterizing phenotypic groups of low-virulence field strains of
The epidemiology of human listeriosis
Molecular determinants of
A spontaneous genomic deletion in
Listeriolysin S, a novel peptide haemolysin associated with a subset of lineage I
In vivo transcriptional profiling of
Antibody targeting the ferritin-like protein controls
Comparative genomics and transcriptomics of lineages I, II, and III strains of
Surface proteins and the pathogenic potential of
Contributions of six lineage-specific internalin-like genes to invasion efficiency of
Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome
Design principles of adaptive immune systems
Evolution and classification of the CRISPR-Cas systems
Interference with histidyl-tRNA synthetase by a CRISPR spacer sequence as a factor in the evolution of
Self-targeting by CRISPR: gene regulation or autoimmunity?
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
Comparative genomics of the bacterial genus
Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen
Comparative genome-wide analysis of small RNAs of major Gram-positive pathogens: from identification to application
Controlling mRNA stability and translation with small, noncoding RNAs
The intracellular sRNA transcriptome of
Identification of new noncoding RNAs in
Identification of small Hfq-binding RNAs in
Comparative transcriptomics of pathogenic and non-pathogenic
Deep RNA sequencing of
Identification of a sigma B-dependent small noncoding RNA in
Genome analysis of multiple pathogenic isolates of
Comparative genomic analyses of seventeen
Organised genome dynamics in the
Evolution of the core and pan-genome of
Characterization and modeling of the
Probing the pan-genome of
Comparative genomics of
High-throughput genome sequencing of two
Invasiveness is a variable and heterogeneous phenotype in
Revival of Seeliger's historical 'Special
Virulence of
GECO–linear visualization for comparative genomics
BIGSdb: Scalable analysis of bacterial genome variation at the population level
Comparative genomics: the bacterial pan-genome
Efficient oligonucleotide probe selection for pan-genomic tiling arrays
The bacterial pathogen
Whole-genome sequence of
Complete nucleotide sequence, molecular analysis and genome structure of bacteriophage A118 of
Cell wall teichoic acid glycosylation in
The ICESt1 element of
Functional studies of a fibrinogen binding protein from
Complete genome sequence of the
Short-term genome evolution of
Prophage excision activates
Microarray-based characterization of the
Identification, cloning, and characterization of the
PILER-CR: fast and accurate identification of CRISPR repeats
CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats
A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes
Mugsy: fast multiple alignment of closely related whole genomes
Genome phylogenetic analysis based on extended gene contents
Assessment of phylogenomic and orthology approaches for phylogenetic inference
Genome sequence of the nonpathogenic
Gene gain and loss events in
Genome phylogeny based on gene content
Pyrosequencing-based comparative genome analysis of the nosocomial pathogen
Contribution of three bile-associated loci,
Adaptation of
A bacterial protein targets the BAHD1 chromatin complex to stimulate type III interferon response
A five-gene stress survival islet (SSI-1) that contributes to the growth of
Glycerol metabolism and PrfA activity in
Inhibition of ROCK activity allows InlF-mediated invasion and increased virulence of
Variation in
Homologous pairs of regulatory proteins control activity of
sigma(B) activity in
RsbT and RsbV contribute to sigmaB-dependent survival under environmental, energy, and intracellular stress conditions in
Fold and function of the InlB B-repeat
Distribution of internalin gene profiles of
Ruminant rhombencephalitis-associated
Molecular characterization of the arginine deiminase system in
Genome diversification in phylogenetic lineages I and II of
Immunity to the bacteriocin sublancin 168 Is determined by the SunI (YolF) protein of
Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution
sRNAdb: a small non-coding RNA database for gram-positive bacteria
Novel factor rescues ribosomes trapped on non-stop mRNAs
Rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry
GenDB–an open source genome annotation system for prokaryote genomes
Augur–a computational pipeline for whole genome microbial surface protein prediction and classification
Internalins: a complex family of leucine-rich repeat-containing proteins in
The Pfam protein families database
Basic local alignment search tool
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
Application of phylogenetic networks in evolutionary studies
Genome trees and the tree of life


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General information about family concerts
For 13 seasons now, the Palau de la Música Catalana has offered the Concerts Familiars al Palau (Family Concerts at the Palau) season to offer a shared musical experience to parents, children, grandparents, grandchildren, brothers and sisters and so on, creating a shared moment of entertainment in a unique setting.
The family productions at the Palau are designed and created for music to interact with other forms of artistic expression, including dance, theatre, poetry or visual arts, with the aim of fostering curiosity, sensitivity, imagination and creativity among old and young.
The Concerts Familiars del Palau de la Música Catalana begin the season with a series of new productions specially designed for all the family to enjoy music together. The traditional concerts which fill our flagship Art Nouveau auditorium and the Petit Palau we add new production: Big Bang Beethoven conceived from the Education Department of the Palau with musical direction by Eduard Iniesta and choreographic direction of Enrique Cabrera. Another new feature this season is the premiere of the hit show Flamenkat in the Concert Hall, a concert-performance that also excite young and old, has managed cross frontiers .
Also, continuing with the idea of putting on musical workshops for the very young, such as Bim Bom, nadons al Palau (for babies aged up to 12 months) and Zig Zag, passets al Palau (for toddlers from 1 to 2 years old), and Abans de néixer al Palau, a musical workshop for expectant parents in which we take a journey into the sound universe of the baby in order to foster the bond with the unborn child. On the other hand, continue concert-shows premiered in recent seasons: Les Músiques the mon; Cobla 2.0, Swing or Jazz viu Kantikipugui Palau among others ...
Finally, with the aim of offering a new look at the architectural heritage of the Palau, we propose that just before one of the family concerts you take a guided tour specially designed for families with children aged 5 to 10 so that they too can discover and experience the visual features of this unique building, a World Heritage Site.
We invite you then to take part in this unique experience, a catalyst for artistic experiences which will make music into a part of the life of the smallest members of the household.
Free with the Super3 card except in workshops and concerts at the Petit Palau.
* You must present your Super3 card at the box office and buy a ticket is required adult to accompany.


text:
Making A Heart For God: A Week Inside A Catholic Monastery
Making A Heart For God: A Week Inside A Catholic MonasteryStyle: BK28
Author Dianne Aprile
Forward by Brother Patrick Hart, O.C.S.O.
197 pages
"In our over stimulated society, Dianne Aprile's glimpse into abbey life offers us a view of spiritual refreshment without rival."
--Rabbi Howard A. Addison Author of Show Me Your Way: The Complete Guide to Exploring Interfaith Spiritual Direction
"Dianne Aprile has breathed deeply the fresh, life-giving air of a remarkable place where God is more than a topic of casual disinterest."
--Timothy Jones Author of Nurturing a Child's Soul and A Place for God: A Guide to Spiritual Retreats and Retreat Centers
A life of quiet, work and prayer, monasticism has been a part of the Christian spiritual tradition for over 1,700 years, and it remains very much alive today. This guide will prepare you for your own first venture into experiencing monastic spirituality-a fascinating way to deepen your spiritual life no matter what your faith tradition.
Weather you're simply curious about what's behind the mystery, or interested in experiencing it firsthand, this is the ideal handbook to understand the monastic experience.
Also included are a helpful glossary of terms and a listing of monasteries throughout North America that receive visitors.
Every purchase helps supply and distribute Vitamin A to Indian children in desperate need. Learn more!
Our Return Policy
We know that you will love what you ordered but if something doesn't work out, we will gladly accept unworn, unwashed or defective merchandise for return or exchange within 30 days from the date you receive your package. Items purchased outside of the United States are not eligible for returns due to customs issues.
How to make an easy exchange:
Ordered the wrong size or color? No problem! Just follow these easy steps to get your exchanged processed. :)
- Place a new order for the exchange items.
- Contact us HERE with:
- Your Original order number
- Items you wish to return
- New Order Number associated with your exchange order. (so we can refund your shipping costs on the new order) Please include reason for return.
- We'll send a confirmation email including a shipping label, and Return Authorization Label.
- Affix label and include Return Authorization Label barcode inside return package.
- Mail return package using our pre-paid USPS label* (if applicable) or shipping method of your choice.
How to make an easy return
Contact us with:
- Your Original order number
- Items you wish to return
- Reason for return
- We'll send a confirmation email including a Return Authorization Label.
- Include Return Authorization Label inside return package.
- Mail return package using a shipping method of your choice.


text:
i decided not to upload my senior project work since Reiq recently got his stuff stolen by some russian 17 year old (enter various lines of profanity here). so im not taking any risky riskssss
ON! a lighter side of the news....I BEAT SONIC 1 and 2! YEY!.....yes i suck at platforming.
AND! i completed Sonic Generations 100% DUN DUN DUN DUN!!!!!!!!!!!!!!!!
- and get sonic CD remade by Christian Whitehead "Taxman"


text:
By Jocelyn McClurg, USA TODAY
When Nathan McCall hollers, people listen.
EXCERPT: Find out about McCall's 'Them'
The criminal-turned-Washington Post journalist grabbed attention in 1994 with his powerful memoir, Makes Me Wanna Holler: A Young Black Man in America.
McCall, who has left the Post and now teaches at Emory University and lives in Atlanta, has written his first novel, Them. Once again, he's raising uncomfortable questions about race (and real estate) in America. Who's Them? For blacks, it's whites, and vice versa.
McCall doesn't write with the spit-and-polish of a Tom Wolfe, but in its own rough-hewn way, Them is as incendiary as The Bonfire of the Vanities.
The spark that ignites the conflagration in Them is the real-life gentrification of Atlanta's Old Fourth Ward, the birthplace of Martin Luther King Jr.
In the past decade, the once "seedy" black neighborhood has been either revitalized or destroyed, depending on your point of view.
McCall tries to present both sides, but his heart is with his black characters, especially his Everyman protagonist, Barlowe Reed.
Barlowe is an intriguing, unusual "hero." He's a prickly sort with peculiar quirks, including a serious aversion to American flags and paying taxes to "Caesar," as he calls the government.
About to turn 40, Barlowe seems stuck on permanent pause. He's unmarried, with a decent job as a printer but only $138 in the bank. His dream of buying a house in his neighborhood appears doomed, with whites moving in and home prices creeping up.
Barlowe's own simmering racism emerges when white couple Sandy and Sean Gilmore buy the house next door.
For many white readers, the hatred Barlowe and his fellow Fourth Ward residents feel for whites will come as a real shock. This is America in the 21st century? But McCall makes us understand and then sympathize with Barlowe, only one generation removed from the Jim Crow South. Barlowe even slowly warms up to Sandy, who is desperate to be friends.
Them excels in depicting, at times with surprising humor, the misunderstandings and mutual distrust that arise when cultures and races collide. McCall is on shakier ground when he veers into violence. It defies belief that the Gilmores wouldn't call the cops after Barlowe's nephew nearly strangles Sean. And Sean's purchase of a gun too loudly telegraphs an unsatisfactory denouement.
Imperfect as it is, Them is provocative, at times heartbreaking. And yet, against the odds, it offers a glimmer of hope.
Conversation guidelines: USA TODAY welcomes your thoughts, stories and information related to this article. Please stay on topic and be respectful of others. Keep the conversation appropriate for interested readers across the map.


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Prices, specials, features and availability subject to change.
Experience apartment living at its best with a host of amenities, modern features, and a prime location at Graham Hill Apartments! Graham Hill has one, two, and three bedroom apartments in Mechanicsburg, PA. Each home features a fully equipped gourmet kitchen. Amenities include large closets, high ceilings, a washer and dryer, and extra storage. The community features a saltwater swimming pool, fitness center, bark park, and a lot more. Graham Hill is close to the PA Turnpike, shops on the Carlisle Pike, and Wass Park. Wass park features basketball courts, tennis courts, and playgrounds. Weis and Giant grocery stores are also nearby. Graham Hill is pet friendly with some restrictions. This community is professionally managed by Property Management, Inc. with an on-site office. Contact us to schedule a tour!
12 Month lease terms. Please inquire with property staff.
Dogs Allowed. Cats Allowed. Pets Accepted. Call for service animal policy.
153 units
Contact Propertyor call
Staff is very accommodating about any request. Constant grooming to the premises keeps them looking very good. Read More Having the opportunity to have gas heat in the winter makes those utility bills much better. The appliances are new and kept in good shape. It's great to have a swim pool in the summer and a fitness room at any time.It's a lovely quiet atmosphere. My friends who visit often comment on the fact that it is so quiet. It is well priced for it's amenities.
July 05, 2014
Thank you for your positive feedback, we are so happy to hear that you are enjoying your home.
- Your Graham Hill Management Team
The staff.
June 24, 2014
We all Thank Your for your kind review! It is our wonderful residents who make what we do so enjoyable.
-Your Graham Hill Management Team
I know and understand that this is apartment living, but I live on an end unit with the stairs for the upstair Read Mores unit on the outside of my living room wall. Every time that the residents go in and up or down the stairs it sounds like a herd of elephants going up the stairs. The construction on the staircases and floors above the apartments could have been better constructed for sound muffling. Other than that, no other problems.
June 24, 2014
We thank you for review. Please know you can contact us at anytime if there is anything we can do to help.
-Your Graham Hill Management Team
I had to move.... the people who lived behind me were heavy smokers and the smoke came in my apartment... I'm Read Moreallergic, have breast cancer (cannot be exposed to unnecessary carcinogen��??s) , and can't stand the disgusting smell. Their character was rather unsavory and I was uncomfortable being near them......
June 24, 2014
We are truly sorry you had an unpleasant experience. We do understand your reason for needing to move and we are sorry to see you gone but wish you all the best in your new home.
-Your Graham Hill Management Team
Superior service - both management & maintenance.
Management knows residence by name.
June 24, 2014
We thank you for your kind review! We truly love what we do and love our residents!
-Your Graham Hill Management Team
Monday-Friday: 9:00am-6:00pm
Property Management, Inc.
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text:
The studentship will cover tuition fees to home/EU level, and will provide a standard living allowance (stipend), currently £12,300 per annum (subject to annual review).
Overseas students are eligible to apply, but will be liable to pay the difference between home/EU and overseas fees.
Note that fees are subject to change annually throughout the programme of study.
The studentship is expected to start in October.
Applicants must be highly motivated and must normally hold at least a first or upper second class honours degree, or equivalent in an appropriate subject.
Candidates whose first language is not English must meet the University's English language requirements.
Further details of the Faculty and research within the Faculty can be found at http://www.napier.ac.uk/fhls/
Applications, including a CV and a brief summary (max 500 words) on how your experience and background would make you a suitable candidate for this research project should be sent to Caroline Graham, The Graduate School, Napier University, Merchiston Campus, Edinburgh EH10 5DJ.
Please quote the reference number of the studentship you are applying for.
The closing date for all studentships is 20 April.
Please note only shortlisted applicants will be contacted.
Informal enquiries can be made to Dr Richard Simpson, email@example.com, 0131 455 2630.
See; PhD Studentship, School of Life Sciences, Napier University, UK


text: I've looked at Toro, eXmark, Deere, & Scag. It's really down to the Deere & Scag. The JD has a 25 hp Kohler EFI, 54" deck & is $8600. The scag is a 52" with 22.5 hp Kawasaki and is $8,300. The JD is freakin HUGE compared to the tiger cat. I'm not sure if that's going to be a problem for me or not , but I do like the compactness of the scag. Im 6'6" 270 and the deere is more comfortable for big guy. I'd have to buy long lap bars for the scag, at a cost of around $200, to be able to fit. I think these are both quality machines, but I've had a thing for Scag. However , the dealer is about 40 mins away. What do you guys think?


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The president of a Montgomery County manufacturing business has been indicted on federal fraud charges.
The U.S. attorney's office reported that Frank Renick, 62, president of Spectrum Pet Care Inc., which manufactures pet food and products, was indicted on three felony counts of mail fraud in an investment scheme.
The indictment alleges that Renick sold over $7 million of investments in Spectrum, including stocks, bonds and promissory notes to over 250 investors in Missouri and other states between 2006 and May 2011.
A federal grand jury handed up the indictment May 30, but it remained sealed until Renick was arrested Monday, June 4.
Renick allegedly induced investors to invest substantial sums of money in Spectrum by making false representations about Spectrum's financial condition and how the invested funds would be used. He told investors that their investments would be used to purchase equipment and to fund operations.
Although some the funds were used for the business, Renick allegedly used substantial portions to repay existing investors and to pay for his personal expenses unrelated to the business. The indictment alleges that investors lost millions of dollars as a result of Renick's scheme.
If convicted, Renick faces a maximum penalty of 20 years in prison and/or fines up to $250,000 on each count.
The case was investigated by the Postal Inspection Service and the Federal Bureau of Investigation. Assistant U.S. Attorney Reginald Harris is handling the case
Charges in an indictment are accusations and do not constitute proof of guilt, the U.S. attorney's office noted.


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Augusta Gregory
Yearly Forecast for 2017
Augusta Gregory is a female celebrity.
She was born on Monday March 15th 1852, in Roxborough, Ireland,.
During 2017, Augusta Gregory has to be ready for major changes. She will be inspired to start new projects or enterprises. Augusta will feel a strong forward push toward new goals. This is a time for vision and planning. Lady Gregory should share her dream with others; make plans, get the necessary support, but, above all, rely on herself as the driving force, and most importantly - be decisive!
Augusta Gregory is starting a new nine-year Epicycle. Everything she does now will affect her future. Augusta must not hold back the inner force of creation. She needs to be direct, daring, and bold.
Lady Gregory will have more confidence and determination this year, particularly in comparison with last year, which was a time of letting go. This year represents a time of birth. It is a time to take charge and to apply herself to her dream. This is also a good time to make the personal changes Augusta Gregory has long wanted to make: start a diet and/or an exercise program, or begin a new course of study.
There may be some emotional turmoil, especially in the first two or three months. It takes a while to get the ball rolling. There are many changes Augusta must make and much work to be done. She should be open-minded, organized, and focused, while avoiding distractions and procrastination.
Lady Gregory is at a crossroads. She will need courage and a clear head to stay on the right track. This is a year of opportunities.
The key months in Augusta Gregory's year of 2017 are March, in which Augusta is able to lay the foundation to her plans; April, in which changes take place such as a change of residence or career; July and August mark a time in which Lady Gregory will see the fruits of her labors begin to take place; October 2017 represents a major turn in events, often fraught with emotional turmoil; the fall marks a coalescing of Augusta Gregory's plans into more concrete form.
Let's take a look now at Augusta's monthly forecast for July 2017.
You and Augusta
About Augusta's Personal Year Cycle
In numerology, each year of Augusta's life is part of an evolving pattern that can be described as a nine-year cycle or epicycle. These cycles begin at Augusta Gregory's birth and progress, one by one, through nine steps that complete an epicycle, and then begin again.
Read more about our numerological yearly forecast for


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Kate Middleton and Prince William Are Feeding Their Baby-Hungry Fans Pastries
Most Popular
In her life as a regular human, Kate Middleton must have waited in line for something she really, really wanted (Spice Girls tickets?), because she knows what you really really really wanna
zig a zig ah do while endlessly waiting: eat snacks.
The Duke and Duchess of Cambridge proved today that they are the most polite and thoughtful man and woman of the people by sending fans waiting for The Birth of the second royal baby "luxury pastries" (mmmmm). Since KMidd is reportedly past her due date, the folks who've been camped out for days, waiting patiently for the Duchess's arrival at St. Mary's Hospital, are probably hungry (or at least bored, which is the reason we eat snacks half the time anyway) and growing frustrated, so Wills and Kate had some sweets delivered to thank them, according to Kensington Palace.
Advertisement - Continue Reading Below
What a sweet gesture! Let's just hope KMidd didn't bake the stuff herself because we know cupcakes aren't her forte:
P.S. Kate, feel free to send us some coffee! Or a Pepcid AC—all this anxious waiting is giving us some serious agita over here.
More from ELLE:


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Orange Marmalade Stove Top Pork Chops
READY IN: 25mins
Recipe by LexingtonMom
Sweet and tangy, low-cal, and quick. Only 5 ingredients! Great with white rice to drizzle the sauce over.
Ingredients Nutrition
- 6 top loin pork chops
- 29.58 ml lemon pepper
- 59.16 ml apple cider vinegar
- 158.51 ml orange marmalade
- olive oil, to coat
Directions See How It's Made
- Brush chops with a little oil and sprinkle with lemon pepper.
- Heat non-stick skillet over medium high heat. Brown chops on both sides until just cooked through. Remove to platter for a minute.
- Add marmalade and cider vinegar to pan, cooking and stirring into baked bits left in pan. Boil for a couple minutes to reduce a little, stirring now and then.
- Return chops to pan and turn once to coat.


text:
Today, I had a meeting at work. My boss was there as well as her boss, and a few other managers and directors. We started discussing politics in the context of our latest project. I tried to say "erratic election". I almost succeeded. FML
BeginnerYou have looked through 5 pages of the website. That's a start.
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It's in the can!Hey, you uploaded your photo, and you're cute as a kitten!
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Today, while sitting on the couch, my boyfriend came over, pulled his penis out of his fly, and started stabbing me in the face with it while humming the Jaws theme. FML
Today, I felt like spicing up our marriage, so I thought I'd surprise my husband when he got home from work. I put on my sexiest teddy, lit some candles, and laid on the bed. He walked in the room, looked at me for a second, farted, then asked me what was for dinner. FML
Today, I dropped my iPod Touch underneath concrete slab steps, and it's physically impossible to get it back. If you stand above where the iPod is, you can still hear it play music. It's like it's mocking me. FML
Today, my schlong decided to enter Mortal Kombat with my pants zipper. Guess which of the two won a flawless victory? FML


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There are many reasons why people choose to buy a condo on a single family residence.
Many parc riviera west coast condos cost less than residential homes; This allows many first time home buyers the opportunity to own property, obtain equity and is still able to live their lives outside the mortgage payments. Treatment: the Most parc riviera west coast condominium complex is managed by the Association of the homeowner or a professional company sub-contracted. Grass and shrubs planted not managed by the homeowner; dues are collected and placed into a reserve account to cover the cost of any large as roof replacement or asphalt paving. The parc riviera west coast owners do not have to worry about exterior maintenance, unlike single-family homes. This saves time in today's busy lifestyles.
Facilities: Some complex parc riviera west coast offers facilities such as swimming pools, sports centers, community buildings and more. The environmental community: Some people enjoy living in an environment able to socialize with their neighbors. Location: Most parc riviera west coast complex is located in a city or town; This allows the owner the opportunity to access all city facilities stay right on their doorsteps. Rules and regulations: some rules are established to maintain order in the complex. For example a rule can be placed that do not allow large RV or boat to be parked in the building; pet restrictions can set the size or type of animal are allowed on the property, so if you are someone who does not like to be around barking dog, a condo with pet restrictions might be right up your alley.
Although there are many advantages, there are also some disadvantages that the buyer may have to consider before buying a parc riviera west coast condo. Rules and regulations: Although the owner has the right to vote on the rules governing the complex, the majority usually wins so you may end up unhappy with the standard rules such as a pet or if you can run a home based business from your unit. Neighbor options: though you rarely get to choose your parc riviera west coast neighbors when you buy, a condo troops close to your neighbors. Most of the time the only thing that separates you from your neighbors is a shared wall.
Tags: parc riviera, parc riviera for sale, parc riviera west coast, parc riviera west coast vale, riviera apartments


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Fingertips - Picture Of My Own (tradução) MP3
Um anjo vem todas as noites, senta-se ao pé de mim, e passa sobre o meu coração, a sua asa mansa, como se fosse o meu melhor amigo. Esse espirito que ...
Fingertips MP3
Fingertips. Audio: "Fingertips" (reprise) by They Might be Giants (pitched down) from their Apollo 18 album.
[Finger Tips] Yummy Cocktail MP3
Leo Kalyan - Fingertips MP3
1st track from the 'Silver Linings EP' Buy on iTunes: smarturl.it/SilverLiningsEP Listen + follow on Spotify: smarturl.it/FingertipsSpotify Follow: ...
Ryan O'Shaughnessy - Fingertips (Official Video) MP3
Fingertips - Official Video Download here: https://itunes.apple.com/ie/album/fingertips-single/id1040951975 - Subscribe for more videos: ...
Fingertips - Pictures of my own MP3
Lyrics: Save me from this sadness it's coming or take me before my smile it's dissolving wake me from this nightmare i'm entering don't let me fall in the corners ...
Fingertips - Melancholic Ballad (Legendado) MP3
Fingertips - Melancholic Ballad Legendado em Português. Espero que gostem e partilhem.
Fingertips (Parts I & II) - Stevie Wonder (1963) MP3
"Just a little bit o' soul...."
Fingertips - Cause To Love You (Legendado Pt-Pt) MP3
Decidi fazer esta tradução porque é uma das melhores músicas que existe e gostava de partilhar com vocês. Espero que gostem!
Fingertips - You're Gone MP3
Beautiful Song.
They Might Be Giants - Fingertips MP3
OK, I don't know if this happened to you guys but everytime I played this there was always this annoying skip in Fingertips Reprise and I'm Having a Heart Attack ...
The Brian Jonestown Massacre - Fingertips MP3
"The collection of music known as Pol Pot's Pleasure Penthouse is from a cassette Anton gave me in 1992 or maybe 1991. It's hard for me to remember the year ...
The Urgency - Fingertips MP3
Music video by The Urgency performing Fingertips. YouTube view counts pre-VEVO: 150357 (C) 2008 The Island Def Jam Music Group.
The UrgencyLove, when you say your faith is falling fast Through your body, your mind and the soul that you claim to have had Well I hoped to move you so and set you free Just b[...]
Emiliana Torrini Fingertips MP3
Emiliana Torrini Fingertips.
How-To Fingerboard Shuvit/Pop Shuvit - FingerTips Tutorial 1 - Disney Exclusive MP3
The shuvit is one of the first tricks most people learn when they start fingerboarding. In this FingerTips tutorial, Taylor Rosenbauer (aka spazpants) teaches the ...
Art attack makes AND Fingertips makes MP3
some other finger tips: http://www.youtube.com/watch?v=Cyr0XxPWisg http://www.youtube.com/watch?v=Gn5SXCgU8ME ...
Fingertips - Out Of Control MP3
Fingertips's new song available in November! #FingertipsOUT "Out Of Control" is the brand new song from Fingertips. With an indie-rock melody, the track was ...
Fingertips - Move Faster (tradução) MP3
O mundo está nas tuas mãos...desperta a tua mente! By ƤΔỮŁØ ₣€ŘŇΔŇĐ€Ş ®™
Hey well, a new video finally! But don't go building bombs now, okay? Original source here: https://www.youtube.com/watch?v=mfMrVKnGzwg.
Fingertips - DO IT MP3
Fingertips (Banjo) MP3
Fingertips #2 This is the second video in our series of 21 shorts based on the collection of mini tracks, "Fingertips" from the album Apollo 18 by They Might Be ...
Little Stevie Wonder - 1963 Fingertips Part II MP3
Tokyo Inspired Nail Art ∞ The World At Your Fingertips w/ cutepolish MP3
The Japanese are heavily influential in all forms of pop culture. They always seem to be years ahead of the game and on the cutting edge of trends. In this video ...
St. John's, Canada Inspired Nail Art ∞ The World At Your Fingertips w/ cutepolish MP3
There is no place like home. In this episode of The World At Your Fingertips, cutepolish taps her ruby slippers and takes us on a journey to her colorful home: ...
Leo Kalyan - Fingertips (cln Remix) MP3
Subscribe & follow Underground Charisma: https://www.facebook.com/undergroundcharisma https://soundcloud.com/undergroundcharisma ...
Oliver Koletzki & Fran - Fingertips (Lovestoned) MP3
ITUNES: http://phobos.apple.com/WebObjects/MZStore.woa/wa/viewAlbum?id=389328235&s=143443 AMAZON: ...
Velvet Fingertips (Original) MP3
This song is now available on iTunes! Please help support my work and buy a song or two! :) For a physical CD, please visit http://www.chriscendanamusic.com ...
Poe - Fingertips (Original Version) MP3
Poe - Fingertips -- J DILLA Beat ---- Sample used from "Like A Lover" by Sergio Mendes LoFi 240p -There is also HD 720p.
Little Stevie Wonder - Fingertips. (Part 1) MP3
Little Stevie Wonder - Fingertips. (Part 1)


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- Feature Links
- Records Request
Records Request
Environmental Record Requests
Reports for underground and above ground storage tanks are maintained through City and County Permitting offices. Below is a list of these offices that are within Eastside Fire & Rescue's jurisdiction, please navigate within these websites to obtain their Public Record Request Forms.
City of Carnation (contact main number City Hall)
King County (unincorporated)
The Dept. of Ecology website will assist you with specific property research associated with Hazardous Material storage.
Injury-Related Record Requests
Please allow 5-10 business days for your request to be processed. Please complete our Authorization Form and return completed form via email or drop off at the Headquarters station:
Eastside Fire & Rescue
175 Newport Way NW
Issaquah, WA 98027
You will need to be prepared to prove identity for HIPAA-Privacy Rules compliance. If mailing or emailing the authorization form, please include a copy of your current valid driver license. For records that include documents associated with transportation billing for a recent incident, requests may take 10-15 business days.
Non-Injury Record Requests
Please allow 5-10 business days for your request to be processed. Please email your request and include the following information:
· Address of response
· Date of response
· Your contact name, address, and phone number
This website is provided as a courtesy to the user. Information contained on the website is not a substitute for the official records and/or files of this Agency. Therefore, neither Eastside Fire & Rescue, nor any officer or employee of the Agency warrants the accuracy of the information set forth on the website. Persons wishing to obtain official records, files, and/or obtain specific information concerning the Agency or a particular incident can also contact the Business Office at (425) 313-3200.


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FISHING industries along the Jurassic Coast could receive an economic boost as an £800,000 fund opens for applications.
The Dorset and East Devon Fisheries Local Action Group (FLAG) fund has officially launched to enhance the economic growth of coastal communities from Swanage to Beer.
The money was secured by the Dorset Coast Forum and Dorset County Council following a lengthy bid process to the Marine Management Organisation for the European Maritime and Fisheries Fund.
The newly-formed local action group will see representatives from within the industries invest the money in fishing and related aquaculture projects to boost the local economy.
Joe Miller, of Swanage and district fishermen's association, said: "The award of the FLAG for the area will hopefully see the facilities around local harbours be brought up to date. It is clear from the work of previous FLAG's in other areas that there are some great benefits available and I hope that the Dorset and East Devon FLAG can bring similar benefits here."
Through local consultations the needs of coastal communities have been assesses and laid out as the Local Development Strategy (LDS).
Local representatives and industry experts will make funding decisions for projects that best meet the needs set out in the LDS
Dorset County Council cabinet member for the environment Peter Finney said: "The FLAG will go a long way to assisting the local fishing communities increase their economic resilience and improve their sustainability moving forward. This fund will allow local people to be involved in projects that will enhance the fisheries and aquaculture sector in the area."
Through the LDS the FLAG also seeks to support the implementation of Common Fisheries Policy reforms.
FLAG coordinator Rhiannon Jones said: "This fund is a great opportunity for local people in the fisheries related sectors to have a say in how eligible projects could help develop the FLAG area's economy. The FLAG board have a wealth of knowledge and experience in these sectors and with their expertise we hope to make a real difference to the fishing industry."
The fund is now open for applications from organisations, businesses and individuals.
Those wishing to apply to should visit www.dorsetforyou.gov.uk/FLAG or call 01305 224833.


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Look and feel
Microsoft's Surface Tab looks smart, although it's a little chunky and hefty for easy one-handed use
Ease of Use
You'll need to learn your way around Windows 8, but once you get used to its layout, it's simple enough to use
The Surface tablet's keyboard cover is a great little device that admittedly not everyone will enjoy. The 1MP cameras are basic, but you get plenty of storage space (expandable) and a full-sized USB port for attaching peripherals
A quad-core nVidia processor provides plenty of power, although we did see the occasional stutter and pause in Windows 8
Battery life
A standard 5 hours of battery life when streaming media is a respectable result
Review by Sunetra Chakravati,3/8/2013 4:23:46 PM
Ease of use
Neat keyboard cover;
Bright screen;
Well specced
Chunky, hefty build;
Windows 8 quirks and lack of apps
Microsoft's Surface Tab is a Windows 8 tablet that thinks it's a laptop, packing a snazzy felt keyboard cover. It's got stiff competition from the likes of the Asus Vivo Tab RT, so how does Microsoft's baby stack up?
As a stand-alone tablet, the Microsoft Surface is your typical black slate. The 'stretched' widescreen design, coupled with the reasonable heft (comparable to Apple's latest iPad), makes it a little cumbersome to wield one-handed in landscape mode. You're best off clutching it in portrait mode. The Surface is solid in all areas, and reassuringly chunky.
If you like 'em chunky, the Surface is your girl
Volume and power buttons are easily accessible at the edges, and Microsoft has even found room for a full-sized USB port on the side, which can be used to add peripherals such as external hard drives. You also get a mini HDMI port for hooking up to a big screen.
However, the Microsoft Surface's unique feature is its keyboard cover and rear stand, which combine to turn this tablet into a laptop. Tug the tablet's back panel and it pops out, propping the Surface up in either portrait or landscape mode – useful by itself if you want to watch a movie without clutching it in your hands. However, in landscape mode you can also snap the magnetic cover onto the bottom. This cover acts just like Apple's iPad accessory – when it covers the screen the Surface hibernates, or you can fold it back behind the tablet to get it out of the way.
On the inside of the cover is a physical keyboard, which kicks into life as soon as the cover is connected. The keys themselves are little more than slightly-raised pads, and initially we were sceptical that it could replace a full physical keyboard or even a virtual onscreen board. However, after an hour of using it to type up emails and even write this review, we found it was a reliable way of quickly and accurately bashing out large reams of text.
Your natural instinct is to push hard on the keys, but you only have to tap lightly for it to register. Once we got used to that we found we rarely made mistakes while touch typing, even with a lack of proper physical feedback. Of course, we'd recommend trying in a store before you buy, and if you can't be without a proper physical keyboard, Asus' Vivo Tab RT may suit you better (although you can also get alternate Surface Tab keyboards that have proper clacky keys if needed).
The keyboard is unfortunately a dust magnet: this is it after extensive brushing
The keyboard has a full range of shortcut keys, including fast access to your charms (menu options), and even includes a miniature built-in touchpad for moving a cursor about the screen – although we still prefer tapping the screen instead. The magnetic bond is also impressively tough, so it's unlikely to come apart unexpectedly.
Our hands-on testing of the keyboard cover's magnets
Windows 8
Anyone who's used Windows on a home computer will notice immediately how Windows 8 looks nothing like previous versions. This version is optimised for touch control, with a desktop filled with 'live tiles' which you can swipe through and tap on. Each tile is a gateway to one of your apps (e.g. music, mail) so tapping on it opens up the relevant app, but the tile itself is also a live information feed – for instance, if you receive an email, the mail tile will tell you so. This saves you tapping it every few minutes to see if anyone's bothered to get in touch.
The tiles aren't limited to just apps, however. You can pin pretty much anything you like on there, be it your favourite website or even your most loved people, so you can quickly contact them and see their social media updates. For nostalgic Windows fans there's also an old-school desktop, which isn't as much fun to use with your fingers, and multiple users can set up their own desktops, so there needn't be any family squabbling.
You can access Windows 8 RT's (a version of Windows 8 more suited to tablets) more useful features by learning its swipe commands. For instance, drag your finger from the left side of the screen to see your recently opened apps, which you can then run alongside whichever other app you have open (for instance, you can bash out an email while checking your calendar). Drag up from the bottom to open a full list of apps, and drag right to open the 'charms' menu, which gives you quick, easy access to your settings and search/share tools. It takes a while to get used to if you come from an Android or iOS background, but once you learn the gestures it's a fairly intuitive system.
Unfortunately there are still some issues with Windows 8. The People app, for instance, sometimes refuses to update with your latest notifications or shows the wrong profile picture for one of your contacts for no discernible reason.
Microsoft's App Store is neatly presented, with apps broken down into categories such as Sport, Music & Video and Games, and then again into top paid, top free and new releases. The only problem is the limited range, compared to rivals such as Android and iOS. Windows 8 will always be playing catch-up simply because of how much longer the other platforms have been established, but we struggled to find more than a handful of decent games, quality restaurant/drinking hole apps were pretty much non-existent and some of our faves such as Flipboard and YouTube were conspicuous by their absence (the third party YouTube apps are woeful at best).
Still, you get the option to try out many of the paid apps before you buy, which is a neat touch and saves you from splashing out on junk. You also get Microsoft's Office suite (Word, Excel etc) to stay productive on the move, which has to be opened from the desktop section. These apps haven't been redesigned for a touchscreen so the menu buttons are fiddly to use, and this was the one time we used the keyboard's touchpad to ensure we clicked on the right thing.
Performance and battery life
A quad-core nVidia Tegra 3 processor backed up by 2GB of RAM runs the show, and for the most part the Surface runs smoothly. Occasionally things ground to a halt, even when we didn't have much on the go – we'd simply be browsing the App Store and tap something and it would pause for five to ten seconds before bringing up what we wanted. This was an infrequent problem however, and we played some of the latest games without any kind of stuttering.
We managed five hours of media streaming on a single battery charge, about average for a modern tablet. You'll get longer if you limit yourself to more basic tasks such as word processing.
Screen and camera
Then there's the Microsoft Surface's 10.6-inch screen, which may lack the supreme sharpness of rivals such as Apple's iPad Retina display, but is crisp enough to make games, movies and photos look good. Colours are less vibrant than on the Asus Vivo Tab RT, a fellow Windows 8 tablet, but look more realistic. The Surface also boasts a powerful maximum screen brightness, which easily overcomes sunlight and other harsh glare.
Rounding off the specs is a pair of basic 1MP cameras, one on the front of the Surface and one on the rear. The rear-facing lens is mostly pointless, taking grainy shots that are barely even fit for Facebook, but the front-facer is fine for Skype chats.
Microsoft's Surface tablet has plenty to offer if you're after a portable device to keep you productive on the move. The keyboard cover works better than expected and saves you lugging a plastic dock around, while the screen is bright and reasonably crisp. Only Windows 8 itself is a slight downer: for every great feature there's a weird quirk, and the Windows Store is still too barren to recommend.


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Kamal Jain wrote:
A challenge we're dealing with is a massive number of small files, so there is a lot of file-level overhead, and as you saw in the charts…the random reads and writes were not friends of GFS.
It is expected that GFS2 would do better in this area butt this does *not* imply GFS(1) is not fixable. One thing would be helpful is sending us the benchmark (or test program that can reasonably represent your application IO patterns) you used to generate the performance data. Then we'll see what can be done from there ....
-- Wendy


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Free Gift Packaging
- Signature blue gift box
- Stylish silver ribbon
- Personal gift message
8mm Cubic Zirconia Square Frame Pendant in Sterling Silver and 18K Gold Plate
Item# 19988120
Orig. $54.99
Now $27.49
Regal and romantic, this fashion pendant will take her breath away. Created in sterling silver, the eye is drawn to the 8mm round cubic zirconia center stone. A squared halo frame of smaller cubic zirconia in warm 18K gold plate borders the center stone and lines double bale, completing the design. A look to treasure, this pendant is finished with a bright polished shine and suspends along an 18-inch cable chain that secures with a spring-ring clasp.
Typographic errors are subject to correction. Merchandise enlarged to show detail and may not always be exactly as pictured. Promotions, prices, styles and availability may vary by store and online.


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