text:
15' Masonboro Skiff Company Macomber 15 Antique and Classic 2012 Yacht for Sale
Yacht Broker Name:
Scott O Schneider CPYB
Broker Phone Number:
Masonboro Skiff Company Macomber 15 Information:
- 15 Masonboro Skiff Company 2012 Macomber 15
- Vessel Name:
- Vessel Length: 15 feet
- Vessel Builder: Masonboro Skiff Company
- Vessel Model: Macomber 15
- Vessel Year: 2012
- Vessel Type: Antique and Classic
- Vessel Price: $ Contact for Price
- Vessel Location: Wilmington , NC
- For additional details please scroll down
Get More Info:
15 Masonboro Skiff Company Macomber 15 2012 Details:
This Macomber 15 is a traditionally built, flat-bottom skiff that was specifically designed and built for its extremely knowledgeable owners. Always kept indoors under her custom made cover and her overall condition is superior in every respect by any measure.
The Macomber design has a solid hardwood framework of white oak and tropical African sapele. It is planked with cypress and fastened exclusively with silicon bronze hardware. Each piece of lumber was hand selected and individually milled for this specific installation.
Other interesting yachts for sale


text:
16.26 acres in Mohave County, Arizona
16.26 Acres of land with Mountain views. Access property from Flathead Road, see maps. Buyer to verify sq.ft., flood plain and utilities. Utilities Inc. water services Sun Valley.
Hwy 95 to Lipan Blvd east, north on Bison Ave, then east on Flathead Rd
Tax Data (Show Tax Data)
Tax data is not available for this listing.
Contact the Seller for more information.
Contact Seller
Fill out this form to contact Seller about this property
Gina Harris GRI ABR
3790 Highway 95 Bullhead City AZ 86442
Bullhead City, AZ 86442
Other Land for Sale in Mohave County
NOTE: The information contained herein is provided as general information only and has been obtained from sources deemed reliable. It is provided without any guaranty, warranty or representation, expressed or implied, made by Lands of America, or any related entity, as to the accuracy or completeness of the information. The information is presented subject to errors, omissions, change of price or conditions, prior sale or withdrawal without notice. Prospective purchasers should make their own investigations, projections and conclusions concerning the information.

subset: commoncrawl


text:
Status: Open
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Harpster John W Dvm practices at 145 South Birch Street, Canby, OR 97013.
Veterinarians offer general and emergency pet care services. Some veterinarians offer 24 hour emergency services-call to confirm hours and availability.
To learn more, or to make an appointment with Harpster John W Dvm in Canby, OR, please call (503) 266-5881 for more information.
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Small Animal Medicine & Surgery
Harpster John W Dvm is a Veterinarians facility at 145 South Birch Street in Canby, OR.


text:
Clifford's Special Cookbook
Created January 2012
Lovingly Compiled by Zelda Hopkins for Clifford Eugene Hudnall II
To my adopted Grandson that I cherish and love very much. I hope you enjoy it and use it as much as I enjoyed making it for you.
Get Your Copy!
Order a professionally printed, perfect bound cookbook with a full color glossy cover and 50 recipes.
Or Read It Now On Your...


text:
28 November 2007,
Price realised GBP 18,500
A jewelled silver-gilt cloisonné enamel ladle and a small kovsh
Cyrillic mark of Orest Kurliukov, Moscow, 1896-1908; the kovsh with Cyrillic mark of Feodor Rückert, Moscow, 1896-1908; and bearing illegible or spurious marks
The circular bowl with glazed and biscuit floral scrolls and waved pattern border, with beading and rope-work detail, plain stem with vari-coloured floral scrolls and blue ground wirework panels in red and white borders, set with two faceted hardstones, marked under base; a small silver-gilt and shaded enamel kovsh with stylized flower heads and geometric designs decorated with hardstones, marked under base; together with a swing-handled bowl, pill-box, sugar-shovel and salt, all marked under base
the ladle 6¼ in (15.7 cm.) high; the kovsh 3½ in. (9.0 cm.) long (6)
New York +1 212 636 2000
London +44 (0)20 7839 9060
Hong Kong +852 2760 1766
Shanghai +86 21 6355 1766
Special Notice
No VAT will be charged on the hammer price, but VAT at 15% will be added to the buyer's premium which is invoiced on a VAT inclusive basis.
Andrew Graham-Dixon on Russia's avant-garde architects and pre-revolutionary artists
Ahead of our 5 June sale in London, five experts share their insights into Russian art of the past century and more
An expert guide to one of the most important events in the art-world calendar, and an index of in-depth features on Venice
Leaving behind Dubai's high-rise heart, Andrew Graham-Dixon goes in search of its low-slung soul


text:
wKinMut: An integrated tool for the analysis and interpretation of mutations in human protein kinases
Sequence analysis (applications)
Protein kinases are involved in relevant physiological functions and a broad number of mutations in this superfamily have been reported in the literature to affect protein function and stability. Unfortunately, the exploration of the consequences on the phenotypes of each individual mutation remains a considerable challenge.
The wKinMut web-server offers direct prediction of the potential pathogenicity of the mutations from a number of methods, including our recently developed prediction method based on the combination of information from a range of diverse sources, including physicochemical properties and functional annotations from FireDB and Swissprot and kinase-specific characteristics such as the membership to specific kinase groups, the annotation with disease-associated GO terms or the occurrence of the mutation in PFAM domains, and the relevance of the residues in determining kinase subfamily specificity from S3Det. This predictor yields interesting results that compare favourably with other methods in the field when applied to protein kinases.
Together with the predictions, wKinMut offers a number of integrated services for the analysis of mutations. These include: the classification of the kinase, information about associations of the kinase with other proteins extracted from iHop, the mapping of the mutations onto PDB structures, pathogenicity records from a number of databases and the classification of mutations in large-scale cancer studies. Importantly, wKinMut is connected with the SNP2L system that extracts mentions of mutations directly from the literature, and therefore increases the possibilities of finding interesting functional information associated to the studied mutations.
wKinMut facilitates the exploration of the information available about individual mutations by integrating prediction approaches with the automatic extraction of information from the literature (text mining) and several state-of-the-art databases.
wKinMut has been used during the last year for the analysis of the consequences of mutations in the context of a number of cancer genome projects, including the recent analysis of Chronic Lymphocytic Leukemia cases and is publicly available at
Current high-throughput resequencing screenings
A broad number of mutations in the protein kinase superfamily have been reported in the literature
In previous publications, we have discussed the preferential distribution of germline pathogenic deviations
wKinMut represents the first resource to provide an integrated tool for the analysis and interpretation of the consequences of mutations in the protein kinase superfamily. The main objective of wKinMut is to aid computational biologists and clinicians to prioritize pathogenic mutations and to understand the mechanisms by which some mutations lead to disease, and particularly, to cancer.
The tool presented here, incorporates information retrieval and prediction approaches and displays information from diverse sources. First, it simplifies the collection of information about the mutations, such as the classification, domain architecture, functional annotations and plausible interaction partners of the kinase. Furthermore, kinase mutations are analyzed in their structural context and mentions in dedicated databases, genotyping studies and the literature that suggest an implication in disease are also presented. Second, wKinMut estimates the theoretical pathogenicity of kinase mutations with three different approaches, including our newly developed kinase-specific method, KinMut
wKinMut has been implemented mostly in Ruby. The functionality is implemented as a workflow accessible through a REST interface that can render the results either in JSON format or HTML. The later constitutes the interface described in this document. Some of data resources that support this system, such as gene descriptions or iHOP interactions, are queried remotely through the internet as demanded; but are then cached to improve subsequent accesses. The server incorporates some additional caching schemes to improve performance in the back-end, by persisting the job results, and in the web interface, by caching the HTML.
Web interface
Step 1: submission of mutations for analysis
The input to wKinMut are non-synonymous mutations in the protein kinase superfamily. The input format should encode the Uniprot/Swissprot accession number, the wild type residue, the position and the mutated residue. Non-standard amino acids and truncating mutations will be excluded from the analysis. An example of this format would be a mutation from Glycine to Alanine in position 719 of the human epidermal growth factor receptor, which is encoded as P00533 G719A. In the following sections, we will use this example to guide the reader through the different result views (Figure
Figure 1
Summary of the different result pages in wKinMut: Example of a Gly-719-Ala mutation in the human epidermal growth factor receptor
Summary of the different result pages in wKinMut: Example of a Gly-719-Ala mutation in the human epidermal growth factor receptor. The figure shows an example of an input to the server (panel a) and the results summary table (panel b). The rest of panels display show the different outputs from the server, including the gene/protein summary tab (panel c), the domain tab (panel d), the structure view (panel e), the pathogenicity assessment (panel f). Information from the databases, the literature and iHop is exemplified in panels g, h and i respectively.
Step 2: interpretation of the consequences of the mutations
The first output the user will get right after submitting the mutations is a summary page with useful information about the requested mutations (Figure
General information about the protein/gene
Information under the 'gene/protein' tab (Figure
PFAM domains
In a previous publication
Mapping the mutations onto structures
To understand the consequences of mutations might have in protein stability and function it is sometimes useful to study the mutations in their structural contexts. However, mapping mutations from sequences to structures is not always trivial
Prediction of the pathogenicity
In wKinMut the theoretical pathogenicity of mutations is assessed by two independent methods, namely SIFT
Mutations in databases
The wealth of knowledge provided by current research is usually stored in databases. A number of them store information about mutations from diverse perspectives. In wKinMut (Figure
Automatic extraction of mutations from the literature
Unfortunately, the databases referred in the previous section do not contain all current knowledge about mutations. Even in the cases where a database record exists, the knowledgebase cannot always store all contextual information. The context is sometimes very important for the correct interpretation of the predictions: experimental conditions, patients' habits and clinical histories, etcetera. wKinMut provides pointers to mentions of the mutations in the literature under the 'Literature' tab (Figure
Automatic determination of interaction partners
wKinMut integrates Protein-Protein Interactions (PPI) gathered from iHOP in the homonymous tab (Figure
wKinMut facilitates the exploration of the information available about individual mutations by integrating prediction approaches with the automatic extraction of information from the literature (text mining) and several currently available databases. wKinMut works as an open accessible web server.
The system offers direct prediction of the potential pathogenicity of the mutations from a number of methods, including our recently developed prediction method based on the combination of information from a range of diverse sources with a machine learning system
In summary, we think that wKinMut constitutes a powerful one-stop shop for the study of the potential pathogenic potential of mutations in protein kinases. As such, wKinMut will be of interest for bioinformaticians and computational biologists that can use the information provided by the server programmatically as part of their own analysis pipelines, and it can be also useful to biologists and clinicians who can browse and explore punctual information easily from the provided interface. We have used wKinMut during the past year for the analysis of the consequences of mutations in the context of a number of personalized cancer genome projects (see
A further development of the presented system would consider the analysis of the downstream consequences of mutations in relation to potential and known post-translational modifications and their interelations (see
Competing interests
The authors declare that they have no competing interests.
Author's contributions
AV and JMGI designed the experiment. MV, JMGI designed the web server. MV, JMGI and AP implemented the web server. JMGI, MV and AV wrote the paper. All the authors read and approved the manuscript.
The authors thank the members of the Structural Biology and Biocomputing Programme (CNIO), especially A. Rausell, D. Juan, I. Ezkurdia and T. Pons, for interesting discussion and comments on this manuscript. This research was supported by OpenPhacts European project (115191-2) and Spanish Ministry of Science and Innovation project BIO2007-6685.
The consensus coding sequences of human breast and colorectal cancers
The genomic landscapes of human breast and colorectal cancers
Patterns of somatic mutation in human cancer genomes
From cancer genomes to cancer models: bridging the gaps
New avenues in protein function prediction
Next generation tools for the annotation of human SNPs
Using bioinformatics to predict the functional impact of SNVs
Predicting deleterious amino acid substitutions
Large scale analysis of non-synonymous coding region single nucleotide polymorphisms
Predicting the functional impact of protein mutations: application to cancer genomics
Human non-synonymous SNPs: server and survey
SNPs, protein structure, and disease
A comparative study of machine-learning methods to predict the effects of single nucleotide polymorphisms on protein function
Distinguishing cancer-associated missense mutations from common polymorphisms
MuD: an interactive web server for the prediction of non-neutral substitutions using protein structural data
Characterization of disease associated single amino acid polymorphisms in terms of sequence and structure properties
SNAP: predict effect of non-synonymous polymorphisms on function
A method and server for predicting damaging missense mutations
Functional annotations improve the predictive score of human disease-related mutations in proteins
Accurate prediction of deleterious protein kinase polymorphisms
Loss of protein structure stability as a major causative factor in monogenic disease
LS-SNP: large-scale annotation of coding non-synonymous SNPs based on multiple information sources
Prioritization of pathogenic mutations in the protein kinase superfamily
Improving the assessment of the outcome of non synonymous SNVs with a consensus deleteriousness score
Extraction of human kinase mutations from literature, databases and genotyping studies
The SAAPdb web resource: A large-scale structural analysis of mutant proteins
Kinase mutations in human disease: interpreting genotype-phenotype relationships
Statistical analysis of pathogenicity of somatic mutations in cancer
The cancer genome
Characterization of pathogenic germline mutations in human Protein Kinases
Cancer-associated mutations are preferentially distributed in protein kinase functional sites
The protein kinase complement of the human genome
Classification and functional annotation of eukaryotic protein kinases
Implementing the iHOP concept for navigation of biomedical literature
Linking genes to literature: text mining, information extraction, and retrieval applications for biology
An integrated approach to the interpretation of single amino acid polymorphisms within the framework of CATH and Gene3D
FireDB–a database of functionally important residues from proteins of known structure
Retrieving mutationspecific information for human proteins in UniProt/Swiss-Prot Knowledgebase
KinMutBase: a registry of disease-causing mutations in protein kinase domains
The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website
Getting personalized cancer genome analysis into the clinic: the challenges in bioinformatics
Exome sequencing identifies recurrent mutations of the splicing factor SF3B1 gene in chronic lymphocytic leukemia
Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia
Deciphering a global network of functionally associated post-translational modifications
Systematic Functional Prioritization of Protein Posttranslational Modifications


text:
Hi Everyone,
We're giving away free stuff to celebrate double rainbows and our new products!
Quantum is counting down to a big product launch on July 27 and we're hoping our new products will end the need to compromise on your backup systems.
To celebrate, we pulled in a turtle, some fish and tapped the double rainbow. http://www.youtube.com/watch?v=HKC73K11xoc
Check out the video and sign up to the Quantum vendor page to go into the draw to win a $50 gift card. We've got 2 to giveaway.
Cheers, Chris
3 Replies
Jul 27, 2011 at 3:42 UTC
Hi Spiceheads,
Exciting times! We've just announced our new DXi6701 and DXi6702 products that include multi-access protocols, industry leading performance, demon-like speed and scalability up the wazoo.
Here's more information: http://qntm.co/DXi6701DXi6702
Gift card winners announced in a few days so follow Quantum's page here for the chance to win. http://community.spiceworks.com/pages/Quantum
I'll post the gift card winners right below here soon ... stay tuned.
Aug 5, 2011 at 12:50 UTC
Ok ... drumrolls, drumrolls ....
The FIRST winner of a $50 Giftcard to BestBuy or Starbucks or Legoland or something else is ..................................................
*dramatic pause*
*short ad break*
And the winner is ......
**annoying dramatic piano-music-pause**
Congratulations and High fives!! Justin, I'll send you a quick email to get your mailing address for the card.
Stay tuned - I have one more $50 gift card to give away next week. Follow Quantum (http://community.spiceworks.com/pages/Quantum) for your chance to win. (We like to giveaway prizes so it's a good idea to follow us)
Aug 22, 2011 at 4:48 UTC
Without further ado ... the next fantastical winner of a giftcard is ...
*** no ridiculous drum rolls here, thank YOU very much ***
Bill Reid
Congratulations, Bill! I'll drop you a quick email to get your mailing address for the card.
That's our last card for this prize draw but stay tuned (and follow us) because I like to giveaway stuff on SpiceWorks. So there'll be more where that comes from!
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
I'm trying to decide between textured or smooth silicone implants. What are the latest reviews on each As of 2014?
Doctor Answers 12
Which Implants To Choose?
My personal preference is smooth implants
Learn more: http://torontosurgery.ca/Toronto-Breast-Augmentation-Enlargement.html
I'm trying to decide between textured or smooth silicone implants. What are the latest reviews on each As of 2014?
In essence it comes down to surgeons preference.
The most important thing is to find a very experienced board certified plastic surgeon to trust and have good rapport with and follow that surgeon's advice.
There are new textured shaped breast implants that are specifically made to increase breast projection in patients who are borderline needing a lift.
On the other hand textured implants have a thicker wall, can be more palpable, and tend not to move as freely as smooth walled implants.
Again find an excellent plastic surgeon and follow that doctors advise. For more information on textured and saline implants please read the following link:
Learn more: http://www.bostonplasticsurgeryspecialists.com/textured-versus-smooth-implants-breast-augmentation/
You might also like...
Benefits of Textured implants
Visit with a plastic surgeon that can make you feel comfortable about the process.
Best wishes
Steven Camp, MD
Choice of implants ( SMOOTH VS TEXTURED) will always be cotroversial
Also need for a lift or no lift is only determined after personal exam by someone who knows art of plastic surgery.
Remember that your goal should not be breast implants. It should be BEAUTIFUL BREASTS. Get a consultation with a plastic surgeon.
Textured implants have the lowest risk in the most recent studies
Trying to decide between textured or smooth silicone implants.
I also agree that seeking consultation with a board certified plastic surgeon should be your next step. Picking a surgeon experienced in dealing with women shaped like you is much more important than worrying about the implant type.
Good luck
Smooth versus textured silicone implants is a very involved discussion
I'm trying to decide between textured or smooth silicone implants. What are the latest reviews on each As of 2014?
Currently, most plastic surgeons in the U.S. prefer smooth implants. You should discuss your concerns with your plastic surgeon, who should be certified by the American Board of Plastic Surgery and ideally a member of the American Society for Aesthetic Plastic Surgery (ASAP).
Robert Singer, MD FACS
La Jolla, California
Should Patient Choose Textured or Smooth Breast Implants?
Because you have been advised to consider a lift, I would first choose a board-certified plastic surgeon with excellent before and after photos. He/she should be someone to trust because you'll need guidance.
Many people want, just as you do, conflicting results: more volume up top without having a lift, despite downward facing nipples. Perhaps a donut mastopexy (nipple lift) and breast augmentation would work. Or it might not. That's why you need a consultation with an excellent plastic surgeon!
Click on the link below to see a breast augmentation case with a nipple lift.
Learn more: http://www.enhanceyourimage.com/photo-gallery/details.cfm?ID=219tub
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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|Home > Entertainment > Article
A kookaburra laughed, too, and a butcherbird spoke. I wondered how many of the species of native birds I could see from Kane's Bridge, or hear. I got 22 in 10 minutes, including two duck species, little and red wattlebirds, cormorants, scrubwren, moorhen, coot, a lonely night heron and a cocky. I did not lie or cheat by counting ravens and gulls, which are always there, but were not.
What were those two holes in the river? Ah, the nostrils of a tortoise. I thought of the country girl who said to me, "My brother smells real good." Puzzled, I asked if he used deodorant. "No," she said, "He's got three nostrils." I wondered why we, and tortoises, have two nostrils. They connect at the back: why not one?
Staying still is the only way I can see frogs during the day. I approach the pool, let them all go plop-plop and silent, then I remain motionless 'till they show their nostrils, climb out to sit on leaves, start up again.
Bloke I know, standing still on a narrow cliff path, had an echidna walk over his boots. It was not alert, none of them are lerts, but most snakes are.
I stared down a tiger snake: me-and-stillness versus it-and-stillness as it hung, half-reared. I won. It collapsed, waved along on its belly into the scrub.
The snake has two nostrils - neither of them for smelling - and one lung. The long, forked, flickering tongue is what it smells with, collecting scent particles from the air, transferring them to organs in the roof of its mouth. Snakes of many species hunt by smell - hence all the flickering - and use sight at close range only. Their eyes focus poorly, but quickly pick up movement. They have no eyelids, just transparent scales over each eye - spectacles. They lack external ears, but respond to ground vibrations, perhaps transmitted by the jawbone to the inner ear.
To grow, they slough their skins, usually whole, with the spectacles in place; marvellous.
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Those who have been anxious for Hank Williams Jr. to release another album don't have to wait much longer. Hank is set to release 'It's About Time', on January 15th, and his fans couldn't be happier.
Hank's new single "Are You Ready For The Country?", was recently released, and gave us a taste of what's to be expected from the album.
As Williams says in an interview with KTIC Radio, the song is already a hit with fans. But he says that what many of them don't realize is that the song has been around for decades, and was a #7 hit for outlaw country singer Waylon Jennings.
"It got real obvious to everybody that, my God, they already think it's a hit," Williams says. "They already think that's yours...they're not putting Waylon together with it...so these twenty-somethings and all, they think that's your song, because it fits so perfectly."
Williams recalls when he used to open shows for Jennings, and how he got to hear "Are You Ready For The Country?" every night on tour:
"Man it means a lot..not to mention the love factor of Waylon, because I'm out there opening shows for him and he starts the show with 'Are You Ready for the Country' back when and he closes the show with a little ditty called 'Are You Sure Hank Done It This Way?'"
Fans got the chance to listen to Williams' version of the song for one of the first times when he opened the CMA's with a performance of it, which also featured Eric Church.
A few weeks later, Williams' music video for the song premiered on CMT. Church also appears in the video, and him and Williams start off performing in what looks like a barn, but later transforms into a parking garage.
Overall, the performance is upbeat and fun-filled, and you can clearly see that Williams and Church are having a blast singing together.
Watch Williams' new music video "Are You Ready For The Country?" off his soon-to-be released album, 'It's About Time' below.
Hank Williams Jr.'s 'It's About Time' is available now for preorder here in iTunes, and will be officially released on January 15th. It's the perfect gift for the outlaw country fan in your life.
Hank Williams Jr. explained to Rolling Stone Country, "We have made an album that is pure magic!" Hank further notes, "The songs — some I wrote and others I got from great songwriters — are typical Hank Jr. songs and I think will connect with anyone who hears them."
See the track listing below, and get excited for Hank's first album since 2012.
Pre-order "It's About Time" now!
Hank Williams Jr., It's About Time Track Listing:
1. "Are You Ready for the Country" (feat. Eric Church) (Neil Young)
2. "Club U.S.A." (Tony Stampley, Bonnie Swayze)
3. "God Fearin' Man" (Chris Janson, Brandon Kinney, Kendell Marvell)
4. "Those Days Are Gone" (Chris Janson, Brice Long, Terry McBride)
5. "Dress Like an Icon" (Hank Williams Jr.)
6. "God and Guns" (Mark Stephen Jones, Travis Meadows, Bud Tower)
7. "Just Call Me Hank" (Hank Williams Jr.)
8. "Mental Revenge" (Mel Tillis)
9. "It's About Time" (Hank Williams Jr.)
10. "The Party's On" (Hank Williams Jr., Joe Kent, Tony Stampley)
11. "Wrapped Up, Tangled Up in Jesus (God's Got It)" (Reverend Charlie Jackson)
12. "Born to Boogie" (feat. Brantley Gilbert, Justin Moore and Brad Paisley) (Hank Williams Jr.)
Interested in becoming a partner?
Contact us for more info.


text:
Have you ever taken a moment to stop and think about your reflection in the mirror? What if reflections had a life of their own. What if it was their job to watch and mimic you?
What if there was a whole new world beyond mirrors? What if your reflection could cross the bridge between the two worlds but is not allowed to?
Well, wonder no more. I am here to tell you my own tale. I am not human. I am the reflection that broke the rules.
It was a hot summer day in 2009. Children outside hanging with their friends. People at the beach and pools swimming. Even the birds were chirping in the trees, making nature's music.
My reflection was collecting his belongings to head home from his afternoon work as a beggar at the local grocery store when a shady-looking character in a suit with a stylish brown fedora carrying a metal suitcase approached him as soon as he had left the store. The look on the stranger's face was full of anger.
The stranger spoke in a husky voice, "You got the cash?".
"No. Not yet." replied my reflection.
"Listen, man. You get me the cash by sunset or I'll have no choice but to use force." He glared at him under his fedora as he spoke.
"I'm trying, I swear... Just - please.. don't hurt me. I'll do my best." The reflection's voice trembled as he spoke.
"NO, THAT'S NOT GOOD ENOUGH! You WILL get it to me!" he had begun shouting.
The reflection flinched and recoiled at the sudden change in the man's tone of voice and quietly replied, "O-okay. I'll see what I can do."
His free hand formed into a tight fist at the reflection's response "SEE?! No, not see. WILL! You WILL get it to me goddammit!" He briefly glanced behind the reflection and noticed people were beginning to stare with concerned looks on their faces.
His gaze turned back to the reflection as he clenched his teeth together but relaxed his hand. He had a look in his eyes that said that he was dead serious about the money. The man realised he was holding his breath and exhaled. A fake smile spread across his face as he tried putting up an act for the people who were watching, shook the reflection's hand tightly, and walked off, briefcase dangling in his hand.
The good-guy get-up seemed to have worked on the customers, as they had gone back to their activities and ignored the previous event.
It was then at that moment that I knew this was going to not go so smoothly. I knew after that conversation who the man was. It was Joun, a worker at the local pawn shop. He and my reflection had gotten into a money dispute in the previous week. He demanded $230,000 from the reflection. I knew that there was no way he could come up with that amount of money that fast as he only makes a mediocre salary.
After a few minutes of processing what had happened, the reflection began walking home, thinking of ways to collect the money before it was too late. The farther he walked, the more he began realising, there was not a single possible way he would be able to gather that amount of money before sundown, and gave up.
Once at his house, my reflection approached a mirror. He is known to talk to me sometimes. If others saw him talk to himself in the mirror, they would think he was crazy. I know he is not crazy.
He placed both of his hands on the counter and stared directly at me through the mirror as I mimicked his every move.
"Looks like it is all going to end here," he paused and took a deep breath. "It was a good run. You've always been there for me."
I wish I could say something back, but I couldn't. It was against the rules. If I broke them, I would seize to exist. My reflection has always come to me when he felt down. When he was in high school, bullies always picked on him for being short. They would beat him down and taunt him in his classes.
He always came to me and talked to me after school. I had always wished that I could help him out but couldn't. The only thing I could do was mimic his every move and repeat everything he said as he talked. There were mirrors all around the school that forced me to watch him get bullied five days a week without being able to do a single thing.
Sunset came quicker than I had thought. The reflection just sat there awaiting the man's arrival.
There was a knock at the door. It was Joun.
The reflection hesitated, but instead, stood up, and approached the door. He placed his hand on the knob, and opened the door.
Without wasting a second, he immediately began, "Where's the money."
My reflection looked up from the ground and stared him in the eyes. His chin began to tremble as he fought back tears "I.. I don't have it..."
"Well, I told you what would happen" Joun reached into his coat and pulled out a pistol.
"You're going to shoot me." The reflection glued his eyes to the gun as his own hands began to shake.
"Nah, not yet. I've got this," and with that, Joun pulled a knife from his pocket with his other hand.
Without wasting another second, he leaned forward and plunged the knife into "my" ribcage. Blood began spilling out of my reflection's body like it was an endless supply. I couldn't do anything but watch. The reflection couldn't take it much longer. He was soon to die. Joun began to laugh as he repeatedly plunged the knife into him. I watched as his hand with the gun began to ascend, then I remembered.
My reflection's rifle is upstairs.
I had two choices here. I could watch my reflection be brutally murdered and do nothing, or I could help him. Although this would result in me breaking the rules and seizing to exist, I couldn't just stand by and watch.
"You should have paid up when you could."
Without any further thought, I transported through the mirrors in the house until I reached the mirror that was in the master bedroom. I began charging my shoulder into what separated me from the other world. Finally, I stumbled through the mirror and landed on the floor. I quickly pushed myself up and stumbled to the dresser. I wrapped my hands around a corner of it and pushed it away from the wall, revealing the rifle behind it. I grabbed it and flung open a drawer. I reached for the shells and began loading the rifle as I ran from the room and made my way to the stairs.
I could hear my reflection downstairs trying to speak to Joun, but his words only came out as gurgles as blood spilled from his mouth. I descended halfway down the stairs, just far enough that Joun and the reflection were in view. Joun had put the pistol to my reflection's head.
He leaned down to be face to face with him "Just don't forget. I didn't choose to do this. You brought this upon yourself" He began to laugh again.
I realised what was to happen next. I leapt down the last few steps and caught Joun's attention, but it was too late. By then, I was standing directly behind my reflection who was knelt on the ground facing the opposite direction. I had the rifle raised and was aimed at Joun. His laugh was cut short. I could tell by the look in his eyes that he was frightened. He had no clue who I was.
I pulled the trigger. He fell to the floor, blood spilling from his head.
I couldn't let my reflection see me. Without hesitating, I dropped the rifle and sprinted back to the mirror that I was originally occupying. Just before climbing back in, I had to say something to my reflection. I disguised my voice and made it significantly deeper.
"Don't give up yet. You are still young and have a lot to live for. We have always been together"
I noticed my reflection had begun to turn around. So I re-entered the mirror and awaited my fate.
This is where I end my journal.
Reflection's Point of View
I had been saved by someone I couldn't see. I guess I do have a lot more to live for than I had thought. With Joun dead I was safe again. There was something after that day that was weird, though. Whenever I approached a mirror, my reflection wasn't there. It was really creepy at first, but I had gotten used to it. I miss my reflection.


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A fairly routine takeout slide on a potential double play ball has turned into a major problem for the Minnesota Twins.
Second baseman Tsuyoshi Nishioka suffered a broken leg Thursday afternoon when he was hit by Nick Swisher of the New York Yankees in the bottom of the seventh inning.
Nishioka, who was signed as a free agent from Japan this offseason, was hitting .208 with one stolen base in his first six games with the Twins.
Matt Tolbert replaced him in the lineup and could fill in occasionally for Nishioka at second. Tolbert is a .246 career hitter with three homers and 14 stolen bases in his fourth big-league season. However, he did hit .311 this spring.
Luke Hughes, who was beaten out by Tolbert for the utility infield spot in spring training, has been recalled from the minors to fill Nishioka's roster spot. Hughes homered in his first major league at-bat last season and led the Twins this spring in at-bats (65), home runs (6) and RBI (15). In deep leagues, Hughes might be worth a speculative pickup because of a higher upside.
UPDATE: Twins manager Ron Gardenhire threw another wrinkle into the situation over the weekend by suggesting that right fielder Michael Cuddyer could see some time at the second base spot.
Cuddyer played the majority of last season at first base due to Justin Morneau's concussion problems. He came up through the Twins system as a third baseman and has played second before, but he hasn't seen extensive action there since 2004.
If Cuddyer can cut it at second, the Twins could put perhaps their best offensive lineup on the field by moving designated hitter Jason Kubel to right field and inserting Jim Thome at DH.
Cuddyer has gotten off to a poor start this season -- hitting just .125 with three singles in 24 at-bats. However, he had 14 homers and 81 RBI last season and eligibility at second base would give his fantasy value a nice boost.
Steve's been with USA TODAY in one form or another since 1993. He started at USATODAY.com in 1996, becoming the website's baseball editor in 1999, and has written the fantasy baseball column for Sports Weekly since 2006. Follow him on Twitter: @sgardnerUSAT.
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Nokia's new flagship N8 smartphoneView gallery - 4 images
Its prototype might not have been found in a bar, but the leaks and rumors surrounding the Nokia N8 can now be put to bed following official outing of the new smartphone. The N8 is the first device to be powered by Nokia's new Symbian^3 platform, and the company believes that it will put some dedicated point-and-shoot cameras to shame with its 12MP camera featuring Carl Zeiss optics and Xenon flash. The N8 can also record videos in HD and edit them with built-in software. It also has an HDMI connector that allows users to display media on HD-equipped TVs and listen in Dolby Digital Plus surround sound.
The Nokia N8 has a 3.5-inch HD capacitive touchscreen and 16GB of built-in storage that is expandable to 48GB with a micro SD card.
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The Nokia blog says the N8 will deliver live Twitter and Facebook updates direct to the phone's home screens, allowing users to comment, read and send messages, update their status and share their location and photos – all with a single touch.
The location-savvy N8 comes with free global Ovi Maps walk and drive navigation with support in more than 70 countries.
Powering the Nokia N8 is the all-new Symbian^3 platform that Nokia says supports gestures such as pinch-to-zoom and multi-touch. Three customizable homescreens can be loaded with apps and widgets and navigated through with the swish of a finger. There are improved 2D and 3D graphics for a faster and more responsive UI, greater memory management and a visual task manager.
Who's a Qt?
This Nokia has full access to the range of Ovi services and is Nokia's first smartphone to be integrated with Qt - a software development environment that makes building apps easy as well as deploying them across the Symbian and other software platforms.
Formed from a single piece of anodized aluminum, the N8 will be available five colors. It will cost €370 (around US$492 at time of publishing) when it starts shipping in the third quarter of 2010.


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Direct and virtually endless highway without opponents who need to overtake and without time limits in which you need to put - that's what racing runner, especially in the case of the game Racing Fever!
There is a game - Traffic Racer, very popular, the number of downloads and over 50 million in itself it has multiple copies, this game is also a copy, but a copy of which was able to, as they say, because any fan of the original game will gladly abandon it for the sake of diversity and new experiences.
Tell a lot about racing runner for android difficult, so it is best to look in detail at the proposed options to spend your time in this game. It all starts with the purchase of the cheapest and slow the car, but it's a push for development and opportunities to earn more money to buy another or upgrading it. Four game modes, the first two differ only in the presence or absence of oncoming traffic, the third mode is a race against time in which you need to keep up to get to the checkpoint which nakinet even a few seconds, giving the opportunity to get to the next, the latest version pokatushek - free ride, it is useful to getting used to the car and just to ride for fun.
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Bottom line: this game is an excellent alternative Traffic Racer, which it copies, and if the developers will modify it a bit, it will be not so much an alternative, as a full replacement for the audience and pick up many times more!
End of holiday campaign
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| view network connections
Allows the app to view information about network connections such as which networks exist and are connected.
| read your contacts
Allows the app to read data about your contacts stored on your device, including the frequency with which you've called, emailed, or communicated in other ways with specific individuals. This permission allows apps to save your contact data, and malicious apps may share contact data without your knowledge.
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Allows the app to prevent the device from going to sleep.
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Allows the app to create network sockets and use custom network protocols. The browser and other applications provide means to send data to the internet, so this permission is not required to send data to the internet.
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Allows apps to accept cloud to device messages sent by the app's service. Using this service will incur data usage. Malicious apps could cause excess data usage.
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Attention! All files represented on this site either were found freely distributed on the internet or by their authors' permission. If any of materials on this site violates your rights, report us
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Don't Let the Riverbeast Get You on Facebook
Just looking at the monstrous merman at the heart of the new indie horror-comedy Don't Let the Riverbeast Get You! reminds me yet again why an old fashioned rubber monster suit possess so much more charm and personality than the majority of today's digital monsters.
That tagline for Charles Roxburgh's low budget Don't Let the Riverbeast Get You! bills its title creature as "the most indecorous aquatic menace of all-time." For those without a dictionary, "indecorous" is another word for unseemly. Not entirely sure why that applies to the Riverbeast because I'm sure if you look hard enough, you'll see the costume's seems. Oops, wrong seams.
I don't know if the movie will be good or bad, but I can't look at the costume without it warming the cockles of my heart. It's goofy and ghastly, wonderfully so.
The film stars Matt Farley (who also wrote the script and produced the film), Sharon Scalzo, Kevin McGee, Elizabeth Peterson, and other friends of director Charles Roxburgh, who told Undead Backbrain they wanted to work together on a fun project that they hope others will have fun watching.
You can find more stills at both Undead Backbrain and the film's Facebook page (hit the link below). A brief teaser trailer gives you a taste of the 1970's style monster comedy that is Don't Let the Riverbeast Get You!
That guy in Cabin in the Woods was right: Everything really is better with a merman.
Got news? Click here to submit it!
Don't let anyone get you in the comments section below!


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
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Why are people visiting my site telling my forms are disabled?Asked by stameliareled on June 13, 2016 at 12:55 PM
People who use my site have been calling to say the form is gone and there is a message that says Form not found. I can see the form on the jotform site but it doesn't show up on my website (www.stameliareled.com)
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The little moments in life mean the most.
This morning Jasmine and I were talking about going through the baby clothes and getting the newborn clothes out in preparation for Connor's arrivial. She looked at me and said "mom can I ask you a question?" I said yes and she asked me if she could come up and see Connor right after he is born. "Mom, I love him so much and I want to see him before hes all hooked up to machines and tubes, before they take him to surgery. I want to tell him how much I love him and how much I want him to come home." I cried. It was such a touching moment. I know I did something right with her. She is so loving and compassionate. She has a deep care for others.
Oh gosh.. that Jasmine.. she has SUCH a sweet heart.. cherish her!
Awww. You raised a good one there. It's crazy how siblings have that connection and as yours before he's even born. What a great kid :)
Brought tears to my eyes too. What a little sweetheart.
Thank you everyone. I had to wait til she left for school, because I did not want her to see me cry and think something was wrong. It is amazing even how my Makenzie, at 2, is already connecting with the baby growing inside of me. She seems to be starting to get that there is a baby in there. Jasmine has a very tender heart, and always has. She cannot stand to see anyone in pain; either physical or emotional. If you are crying, she is too.
tears in my eyes... Amanda, what can I add? You should be proud of your daughter... And of yourself - only such an amazing loving person as you can build such family!
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About Amanda Hurley
Current: Flat Rock, Michigan
Birth: October 04
On Moms.com since: Sep 20, 2013
I am a 28 year old SAHM of four children. Mr. Connor is my son. I am looking for some additional help for a permanent grave marker. If you would like to help with expenses or would like to send a card, please inbox me for an address and details. Thank you
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Establishing a solid energy policy is one of the most important roles that Congress has. Legislation that simply raises taxes on American oil companies will not alleviate the problem. In fact, it will only make the burden heavier for consumers. I believe we need to implement legislation that will encourage conservation and promote the research and innovation necessary to develop new technologies. But, while doing this, we must also unlock new oil supply. Texans still put gasoline in their cars and trucks so we can't expect new technology to take the place of oil and gas overnight. I believe that creating a renewable energy portfolio-wind, solar, hydropower, geothermal and ethanol-while maximizing our use of domestic fossil fuels-coal, oil and natural gas-will allow us to plan for our future needs while providing enough energy for today.
While our nation may require the use of fossil fuels for some time, renewable sources of energy also have great potential to alleviate our energy shortcomings. I strongly support the federal government's initiative to provide grant funding for research and development of new technologies.
Our nation's dependence on foreign sources of energy poses both an approaching supply problem and potential threat to our national security. I firmly believe we must reduce our dependence on crude oil from countries, such as Iran and Venezuela, who wish America harm. By exploring alternative and traditional sources of energy within the jurisdiction of the United States, our energy independence and national security can be improved.
Source: http://carter.house.gov/index.cfm?sectionid=153§iontree=5,87,153


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Military Intelligence and You!
This satirical comedy weaves vintage military training films and footage from World War II movies with a modern-day story about Major Nick Reed (Patrick Muldoon), whose mission to find a hidden enemy base is hampered when he runs into his ex-lover and her new man. Assigned to track down the Ghost Squadron, the Army Intelligence analyst is sidetracked by Lt. Monica Tasty (Elizabeth Ann Bennett) and her boyfriend, Major Dunning (Mackenzie Astin).
Rent DVDs for only $4.99 a month.
Movie Details
- Director
- Dale Kutzera
- Genres
- Comedy, Spoofs and Satire, Indie Comedies
- Format
- Ratings
NRNot rated. This movie has not been rated by the MPAA.
Widescreen 1.85:1Subtitles
NoClosed captioned
NoLanguage and sound
English: Dolby Digital 5.1Other features
Filmed in B&W; interactive menus; scene access.
Military Intelligence and You!Close
For Parents
Common Sense Note
- Age appropriate
- Not an issue
- Depends on your kid and your family
- Not appropriate for kids of the age most likely to want to see it


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|Home > Wibeke
♀ Wibeke
What does Wibeke mean?
Wibeke [wibeke] as a girls' name is of German and Scandinavian origin, and the meaning of Wibeke is "alive". Variant of Viveca.
ASSOCIATED WITH scandinavian, alive (life)
VARIANTS Vibeke, Wiebke, Wiweca
RELATED FORM VIA VIVECA VivekaCREATIVE FORMS
(female) Wibebe, Wibete, ..
(male) Wibeer, Wibefe, .. MIDDLE NAME PAIRINGS
Wibeke Carlie (W.C.), ..
How popular is Wibeke?
Wibeke is an uncommon first name for females. Wibeke is a rare surname too for all people. (2000 U.S. CENSUS)
Wibeke has yet to be ranked in the Top 2000 so far. (2015 BIRTH Statistics)
Similar NamesSuggested similar names are Anneke, Eike, Mike, Milke, Nike, Rebekke (see Rebecca), Rikke, Rivke, Silke, Tineke, Wileen, Willene, Willie▼, Wilone, Winne and Winnie▼. These names tend to be more commonly used than Wibeke.See names in meaning and etymology.


text: I'm Dr. Guichet and I joined the WikiWorldBook community to find the best answers to questions plaguing sleep apnea doctors in Orange County. I have been a professional in this field for decades and first started after graduating from the Georgetown University School of Dentistry. I work with organizations such as the American Mensa, ADA, Orange County Dental Society, and the Academy of Osseointegration.


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Factory Rear CV drive shaft? (Double cardon)
Discussion in 'The Garage' started by camarossguy2, Jul 27, 2006.


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CEO and president of NBC parent NBC Universal, Stephen Burke, says the move is aimed to have Bell 'take all his time to keep [Olympic] coverage on the cutting edge.'
NBC has confirmed Jim Bell's impending departure from "Today". In a statement released on Tuesday, November 13, the network explained that Bell would leave his position as the morning show's executive producer to serve as the full-time executive producer of NBCUniversal's coverage of the Olympic Games.
"There is no doubt that Jim is the right person to be our Olympic production leader into the future," said NBC Sports Group Chairman Mark Lazarus, whom Bell will report to in the future.
Lazarus continued praising Bell, "His professionalism was unmatched in leading the London Olympics to record levels. Jim has worked with the best in the Olympic television world, and we���re excited to have him join us as the full-time executive producer."
President of NBC Olympics Unit, Gary Zenkel, chimed in, "The Olympic legacy at NBCUniversal is long and rich, and Jim has had a role with this unit going all the way back to Barcelona in 1992. It will be great to have Jim back full time as part of our incredibly talented and experienced production team."
Meanwhile, Stephen Burke, CEO and president of NBC parent NBC Universal, claimed the reshuffle was intended to have Bell "take all his time to keep [Olympic] coverage on the cutting edge." He additionally gushed, "We're going to experiment."
NBC hasn't announced Bell's replacement, but rumor has it, Alexandra Wallace is expected to be named as the new executive producer of "Today". The 46-year-old is currently serving as the executive producer for "Rock Center with Brian Williams".


text:
"Gamekeeper? Hmm ..." said Professor Dippet, slowly. "But, Albus, if he is the boy who opened the Chamber of Secrets –"
"He has said he is not, and I believe him," said Dumbledore evenly. "I have a suspicion, however, that the real culprit has been brought into line. So I hope."
"So we all hope," Dippet agreed unhappily. "This whole business has been dreadful ... that poor girl, and her parents ... well. Any more incidents like that and the school could be closed. You really think it is all over with?"
"I think," said Dumbledore, "that we were talking about hiring Hagrid as gamekeeper. And I also think that he had nothing to do with Myrtle's death. There is no deception in him, you know, Armando. Do you really think that the Heir of Slytherin – if such a person exists – would be someone like Rubeus Hagrid? The boy means well, but he never has been Hogwarts' cleverest student. He can barely manage his homework, much less his exams."
"Even so," said Dippet doubtfully, "perhaps it would be best if he went away from the castle. That is supposed to be what expulsion means. Nobody wants to see him around here any more, too much bad feeling ... and he has been accused of a dreadful crime. Why would he want to stay?"
"Because this is the only place he belongs," replied Dumbledore. "Listen, Armando: he has no other home. His father has died, and his mother is ... not present. He has found happiness here. Let him stay. Where else is he going to go? Can you imagine him trying to make his way among Muggles?"
Dippet was silent for a moment, as if picturing the oversized, clumsy thirteen-year-old Hagrid squeezed into a suit, working a desk job at some Muggle office. Was that possibly the shadow of humor on old Dippet's tired face?
"We aren't running a charity ward here, Albus. I know you like to bring orphans to Hogwarts and pay their costs out of the school's budget –"
"—and they return the favor, in the end," said Dumbledore firmly. "They grow up to become fine, productive wizards after they've been through Hogwarts, and later on they earn the gold that helps keep the school running. Instead of making trouble with untrained magic and causing headaches for the Ministry, which they would do if they had not been taught better. Hagrid will earn his way, if he stays here. You can't deny we need a good gamekeeper, somebody who can handle hard labor and isn't afraid of the Forbidden Forest. The house-elves have enough to do indoors, and the forest residents frighten them."
"Well, Hagrid does seem to have an ... affinity for magical creatures..."
"Precisely," said Dumbledore, smiling. "Rest assured I've given him a good talking-to about the werewolf incident. It won't be happening again."
Dippet squinted at his Transfiguration teacher. "You will keep a close eye on him, then, Albus?"
"Yes, I will. I promise you that. He won't be in my House any more per se, since he is no longer a student, but I will gladly vouch for his behavior. If the school governors complain about him, you can feel free to blame it all on me." Dumbledore smiled slightly. "Anyway, they don't need to know about who is helping to trim the grass and feed the giant squid ... they are mainly interested in the welfare of our students, not our staff."
Dippet nodded. "Very well, then. He will be gamekeeper. Though I don't know where is going to live, Albus."
"Oh, I've thought of that," said Dumbledore serenely. "Remember that old cabin Madam Hooch has been keeping the Quidditch things in? We can have that fixed up in no time."
"But what about the brooms and Bludgers and so forth? Where are we going to put them?"
Dumbledore laughed. "Easy enough, Armando. We'll just stuff them into Slytherin's secret Chamber, once Hagrid tells us where it is."


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Both the writ petitioners were included in the ranked list for appointment to the post of Non Vocational Teacher (Junior) in the Kerala Vocational Higher Secondary Education Department. Ext.P1 is the copy of the ranked list dated 18/12/2007. They were included as rank Nos.59 and 60 in the supplementary
list of Scheduled Caste candidates
Goto indiankanoon


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You've already got 5 items in your shopping cart, so there's no room to reserve another one. If you'd like, you can take something out of your cart to make room for this one.
Play Trailer
Me Before You
| ||
1:50 min|
Redbox Play Pass eligible
Plot Summary
Plot: Louisa "Lou" Clark lives in a quaint town in the English countryside. With no clear direction in her life, the quirky and creative 26-year-old goes from one job to the next in order to help her tight-knit family make ends meet. Her normally cheery outlook is put to the test, however, when she faces her newest career challenge. Taking a job at the local "castle," she becomes caregiver and companion to Will Traynor, a wealthy young banker who became wheelchair bound in an accident two years prior, and whose whole world changed dramatically in the blink of an eye. No longer the adventurous soul he once was, the now cynical Will has all but given up. That is until Lou determines to show him that life is worth living. Embarking together on a series of adventures, both Lou and Will get more than they bargained for, and find their lives—and hearts—changing in ways neither one could have imagined. Based on the novel by Jojo Moyes.
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|Specific References
|Related Products
|Baculovirus-Insect cells
|Baculovirus-Insect Cell lysate that Mouse TC-PTP / PTPN2 (aa 2-314) transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
|A DNA sequence encoding the mouse PTPN2 (Q06180-1) (Ser 2-Asn 314) was fused with a polyhistidine tag at the N-terminus.
|The recombinant mouse PTPN2 consists of 332 amino acids and has a calculated molecular mass of 38.7 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
|Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
|Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
|12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
|Samples are stable for up to twelve months from date of receipt.
|1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
|1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
|Store at 4℃. After re-dissolution, aliquot and store at -80℃.
|Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.


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My First Memories of Being a Student2013-10-12 5 Share Tweet
I was five years old when my mom first sent me to school.
In June 1993, I attended the then newly-opened, small pre-school just a block away from our old house in Pateros, Metro Manila. So basically, that makes us the first batch taught by our teacher named Maricar and her assistant Lisa.
Given that two decades have already passed since then, there are now only a few memories that I can somehow remember clearly. These are some of them.
These photos were taken during our field trips. I'm pretty sure the ones taken at the zoo and the science center were from a different date than those taken in an indoor, pseudo-animal farm and the aquarium. The kids with me in some of the snapshots were two of my closest friends back then, Jamyl and Ismael.
This is a random snapshot of me and my classmates having fun in school. I thought I had more photos of such moments; unfortunately, I was unable to find them in the photo albums that we still have at home.
These are photos taken during our Christmas Party. I remember it being held at the rooftop of a small building in our town, with a bank located at the first floor. Anyway, this is one of the clearest memories I still have of kindergarten. I remember that we were made to sing "The Christmas Alphabet" in front of our parents, and that my line was "A is for the angels who make up the Christmas list"! A man dressed as Santa Claus was at the party, too, and gave us presents (that were, of course, from our parents!).
Lastly, these are some of the more decent shots we have of my graduation day. Decent, because the other photographs turned out too bright because of the sunlight. I don't really recall much about this day, but seeing that photo of me and my classmates in funny hip-hop clothing (I'm the one in the orange shirt) reminded me of the days that we had to memorize our dance steps for the then very popular song "Macarena." Now that I think more of it, our teachers back then liked to make us hold performances for our parents showcasing dances to the tune of pop music!
Unfortunately, I heard that my old school closed its doors a few years ago. From what I know, my teacher has already shifted careers and is now a chef. But up to this day I can still recall little things, like the bakery shop at the corner of the street where my school was that my mom buys me cupcakes from. How I cried one time because my mom wasn't there when class was dismissed, but it only turned out that she bought me a slinky before getting me. Some of my classmates there eventually became my school mates when I transferred to the Catholic school also just a stone's throw away from our home. I may not remember a lot anymore, but I can confidently state that I did have a lot of fun when I was in kindergarten!
This month is dedicated to #MakingMemories with Lomography. Raid your attic, blow the dust off your photo albums, and share your favorite analogue memories with the world. Scan those photos and upload them to Lomography and your social media accounts, using the tags #MakingMemories and #Lomography. It's time to celebrate the good old days through the magic of analogue photography!
written by chooolss on 2013-10-12 #lifestyle #school #kindergarten #student #kinder #makingmemories #analoguechildhood


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Gov. Chris Christie (R-N.J.) will run for reelection in 2013, political adviser Mike DuHaime confirmed. While he was expected to run for a second term, it was not a sure thing.
Newark Mayor Cory Booker is seen as Democrats' best chance to beat Christie, whose popularity has surged in the wake of Hurricane Sandy. The storm also pushed back Booker's timeline for making a decision. He said recently that he plans to decide by mid-December.
Even before the storm, Christie enjoyed favorability ratings above 50 percent. A recent poll suggests that only Booker and former governor Dick Codey (now a state senator) have the name recognition and popularity to beat Christie. State Sen. Barbara Buono is also aiming to take on the governor.
Christie beat then-Gov. Jon Corzine (D) in 2009 by about 87,000 votes.
The pugnacious governor flirted with a presidential bid this past cycle before backing former Massachusetts governor Mitt Romney. If he wins reelection, Christie will be well-positioned to run for president in 2016, although there was some grumbling from Republicans about his embrace of President Obama in the wake of the devastating hurricane.


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Quintanilla Tire Ctr, Tires - Retreading, listed under "Tires - Retreading" category, is located at 3342 S Halsted St Chicago IL, 60608 and can be reached by 7738903840 phone number. Quintanilla Tire Ctr has currently 0 reviews.This business profile is not yet claimed, and if you are the owner, claim your business profile for free. If you are not the owner you can invite this business to join by email or by phone.Browse all Tires - Retreading in Chicago IL. Discover census data for Chicago, IL.
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Ratings body OBSR is set to meet with the Baillie Gifford High Yield Bond fund team, after manager Ben Thompson announced he is leaving the industry.
By Lora Coventry, Analyst, Financial Express Thursday May 20, 2010
OBSR will not take any immediate action on the fund's AA rating, it says, as investment managers within the fixed income team, Rob Baltzer and Donald Philips, are set to take over at the Baillie Gifford High Yield Bond fund after Ben Thompson's departure in June.
Performance of fund vs sector over 1-yr
Source: Financial Express Analytics
"The fixed income team at Baillie Gifford works with a strong team ethic and therefore this management change does not represent a change in investment approach. However, OBSR will meet with Baillie Gifford shortly and will reflect any change in its view thereafter," the body says.
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Claire Messud's new novel, The Burning Girl, feels somehow different from anything she's done before...
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"Americans can't get enough of 'Downton Abbey,'" said The Boston Globe. As Season 3 of the award-winning TV series opens, it is 1920 and Downton Abbey is waking up to a world changed forever by World War I. New characters arrive and new intrigues thrive as the old social order is challenged by new expectations.
In this new era, different family members abound (including Cora's American mother, played by Shirley MacLaine) and changed dynamics need to be resolved: Which branch of the family tree will Lord Grantham's first grandchild belong to? What will become of the servants, both old and new?
The Chronicles of Downton Abbey, carefully pieced together at the heart and hearth of the ancestral home of the Crawleys, takes us deeper into the story of every important member of the Downton estate. This lavish, entirely new book focuses on each character individually, examining their motivations, their actions, and the inspirations behind them. An evocative combination of story, history, and behind-the-scenes drama, it will bring fans even closer to the secret, beating heart of the house.
MATTHEW STURGIS is a writer and critic who has written for Harpers & Queen, The Sunday Telegraph, and The Independent on Sunday. He is the author of Passionate Attitudes: the English Decadence of the 1890s and the highly-praised Aubrey Beardsley. He lives in London.
JULIAN FELLOWES is the creator, writer, and executor producer of Downton Abbey, which won six Emmy Awards and the Golden Globe for best mini-series. Previously, he won the Academy Award for best original screenplay for Gosford Park, and wrote the bestselling novels Snobs and Past Imperfect. A member of the House of Lords, he lives with his wife and son in London and Dorset, England.


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Event, Conference and Corporate Photography Services - Melbourne, Australia
We will look after your photographic needs including headshots for your web gallery and brochures, your staff awards presentation or party, your celebration of the special events in your life, to your next major conference event. Our experience and expertise ensures you receive images that really click with the results you expect. We'll provide a fast turn-around without sacrificing quality.
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Take a look at the Conference photos in our portfolio to get an idea of the standard and attention to detail we offer. Our quality is superb.
After the event we'll set up an online gallery so your conference can be shared with delegates and sponsors. Downloading image files through the online gallery is a simple process.
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