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Ultra-high throughput sequencing-based small RNA discovery and discrete statistical biomarker analysis in a collection of cervical tumours and matched controls
Ultra-high throughput sequencing technologies provide opportunities both for discovery of novel molecular species and for detailed comparisons of gene expression patterns. Small RNA populations are particularly well suited to this analysis, as many different small RNAs can be completely sequenced in a single instrument run.
We prepared small RNA libraries from 29 tumour/normal pairs of human cervical tissue samples. Analysis of the resulting sequences (42 million in total) defined 64 new human microRNA (miRNA) genes. Both arms of the hairpin precursor were observed in twenty-three of the newly identified miRNA candidates. We tested several computational approaches for the analysis of class differences between high throughput sequencing datasets and describe a novel application of a log linear model that has provided the most effective analysis for this data. This method resulted in the identification of 67 miRNAs that were differentially-expressed between the tumour and normal samples at a false discovery rate less than 0.001.
This approach can potentially be applied to any kind of RNA sequencing data for analysing differential sequence representation between biological sample sets.
Since the discovery that small RNA effectors define a number of developmental transitions and biological defence mechanisms
miRNA genes were first discovered by forward genetic methods. These methods led to the identification of several miRNA genes associated with developmental phenotypes in
Small RNA profiling by deep sequencing has been applied in an increasing variety of biological situations (for example,
miRNA profiling by small RNA cDNA library sequencing
We captured, amplified and sequenced 58 small RNA libraries prepared from 29 pairs of cervical cancer tissues and matched normal tissues (Additional File
Additional file 1
Small RNA sequences obtained from 29 pairs of human cervical cancer tissues and matched normal tissues.
Click here for file
Additional file 2
Known and novel microRNAs expressed in human cervical cancer tissues and matched normal tissues.
Click here for file
Additional file 3
Distribution of sequence counts for number of unique microRNAs expressed in cervical cancer tissues and normal cervices.
Click here for file
The sequence data reveal a very broad range of expression levels for known miRNAs (based on sequence counts): ~6% of miRNAs were detected at high sequence counts (>104), 14% were in the intermediate range (103-104), and the remaining were at low sequence counts (<100) (Additional File
Novel miRNA genes
To search for novel candidate miRNAs, we used criteria as previously described
Sixty-four novel miRNA genes (88 distinct mature miRNAs) were identified from a total of 45,299 novel sequence reads (Additional File
Forty-one of the new miRNAs were located in introns, one in an exon, and five in the 5'/3' untranslated regions of known genes; 17 were found in the intergenic regions. Notably,
Additional file 4
Novel candidate microRNAs identified from human cervical cancer and normal cervices.
Click here for file
Additional file 5
A unique small RNA downstream of the Vault transcript.
Click here for file
miRNA data analysis
Sequencing-based miRNA profiling does not provide absolute measurements of miRNA expression, but rather the relative counts of different miRNAs within each sample. As described in the previous section, miRNA-seq data are typically characterized by variances in total counts for each sample. These, as well as sequence counts for individual miRNAs, will be subject to large sampling noise. Moreover, in contrast to microarray data, the miRNA-seq data involve non-negative counts.
All statistical analyses were performed on the cube-rooted data, unless otherwise specified. The raw data had a very skewed distribution, with many large values.
The cube-root transformation reduced this skewness and gave the resultant data an approximate Poisson distribution, which was important for our log-linear modelling. The standard approach for testing differential expression of genes measured on microarrays is to compute a
In order to evaluate reproducibility between replicates, we prepared two additional (duplicate) libraries for which small RNA capture, amplification and sequencing were carried out independently (and at a different time) from the respective original samples. The two samples for which this was done were G699N and G761T. From the raw data, we saw correlations of 0.8966 between the two libraries from G699N and 0.7836 for the two libraries from G761T. For reference, the mean correlation between pairs of different normal tissue samples was 0.6708, with the mean correlation between pairs of samples from different tumours being 0.5735. The observed duplicates are by no means perfectly concordant between replicate samples; in addition, we noted that some non-replicate pairs show more correlation with each other than do the pairs of replicates.
In order to visualize the 714-dimensional vectors of miRNA expression in a lower-dimensional subspace, we performed principal components analysis (PCA). The principal components are the linear combinations of the miRNAs that have the largest variance and provide informative axes for projection of the data. The analysis revealed clear separation between tumour and normal samples, but not between tumour types (Figure
Figure 1
Distinctive patterns of miRNA expression between cervical cancer and normal samples revealed by principal component analysis
Distinctive patterns of miRNA expression between cervical cancer and normal samples revealed by principal component analysis. microRNA incidence values from each sample were projected onto the first two principal components, using cube-rooted data. This two-dimensional representation of the ~714 dimensional primary data resulted in evident separation between normal and tumour samples but not between adenocarcinoma (ADC) and squamous cell carcinoma (SCC) samples. The first principal component explains 21.2% of the variation present in the data and the second explains 11.6%. ASC, adenosquamous cell carcinoma; T, tumour; N, normal.
In order to assess the difference between normal and tumour samples, we performed an unsupervised hierarchical clustering of the samples using complete linkage and correlation-based distance. Hierarchical clustering groups the samples by their similarity, in a bottom-up fashion. As shown in Figure
Figure 2
Clustering analyses of normal and tumour samples based on microRNA expression
Clustering analyses of normal and tumour samples based on microRNA expression. (a) Samples were clustered using cube-rooted data and correlation-based distance (as described in Additional File
In order to classify samples based on miRNA expression levels, we applied the nearest shrunken centroids (NSC) method
Additional file 6
microRNAs (miRNAs) used in the nearest shrunken centroid classifier for normal versus tumour, as well as the standardized centroids for each of those miRNAs in each class.
Click here for file
Additional file 7
microRNAs (miRNAs) used in the nearest shrunken centroid classifier for normal versus adenocarcinoma versus squamous cell carcinoma, as well as the standardized centroids for each of those miRNAs in each class.
Click here for file
Additional file 8
microRNAs (miRNAs) used in the nearest shrunken centroid classifier for adenocarcinoma versus squamous cell carcinoma, as well as the standardized centroids for each of those miRNAs in each class.
Click here for file
In order to further explore the performance of NSC for normal versus tumour samples, we randomly split the samples into a training set of 40 samples and a test set of 18 samples. We trained NSC on the training set and tested on the test set; this was repeated 100 times. This resulted in an average of 1.77 errors for normal versus tumour classification. The samples that were most frequently misclassified were G529N, G696T, G701T, G850N and G871T. Not surprisingly, the samples are located near the boundary of the tumour and normal samples in the principal component analysis (PCA) plot (Figure
In order to identify miRNAs that are differentially expressed between tumour and normal tissue, we needed to address the fact that the data were characterized by a high variance in sequence counts between samples as well as between miRNAs, and the fact that the data were discrete. For this purpose, we propose the use of a Poisson log-linear model. In this model, the cube-rooted counts for each miRNA for each sample are taken as Poisson random variables and the logs of the means of these Poisson variables are estimated using a linear model (see Additional File
Additional file 9
Supplementary description of statistical analysis.
Click here for file
Figure 3
Comparison of false discovery rate estimates based on different statistical methods
Comparison of false discovery rate estimates based on different statistical methods. False discovery rates are shown for our proposed method for identification of significant microRNAs (miRNAs) - a log-linear model on cube-rooted data - as well as three competing methods: a log-linear model on raw data,
Table 1
miRNAs with significant changes determined by our Poisson log-linear model
† can be considered as a score for whether microRNA
§ Only microRNAs with low FDR (≤ 0.0001) are shown.
FDR = false discovery rate.
Additional file 10
The false discovery rate of all microRNAs as determined by our Poisson log-linear model.
Click here for file
Dependence of analysis on sequencing scale
Variation in overall sequence depth for different samples will be present in any analysis of independent biological specimens due to differences in tissue makeup and abundance, with some additional variation due to technical aspects of library construction and sequencing. Our discussions above make use of datasets with numbers of sequence reads for the different samples that range from tens-of-thousands to over a million (Additional File
Additional file 11
The number of microRNAs found to be differentially-expressed at a given false discovery rate threshold for each of the resampled data sets.
Click here for file
These results indicate that conclusions can be drawn at a variety of experimental scales, with deeper library coverage resulting in more power for some statistical analyses but with biological factors (for example, number and uniformity of specimens) eventually exerting the major limitations on interpretation as sequencing depth increases.
Analysis of clustered miRNA expression
We defined a miRNA cluster as a set of miRNAs located (i) within a 1 kb region or (ii) in close proximity (<4 kb), with the same orientation and not separated by a miRNA in the opposite orientation. Our data contained 56 miRNA clusters consisting of 236 miRNAs (Additional File
Additional file 12
Average correlation of microRNAs within each cluster, and corresponding
Click here for file
In order to determine whether any of the 56 clusters contain miRNAs that are significantly associated with the cervical cancer versus normal class labels, we applied the log linear model and calculated the median scores for the expressed miRNAs in each cluster. We found that 30 clusters contain expressed miRNAs that are significantly associated with the disease (
Additional file 13
Median score of microRNAs within each cluster in log linear model, and corresponding
Click here for file
Assessment of miRNA expression patterns
We compared the expression level of
Additional file 14
Comparison of sequencing and Northern data of the 29 cervical cancer samples studied.
Click here for file
In this work, we used high throughput sequencing approaches combined with statistical analysis in order to comprehensively characterize miRNA expression profiles of 29 matched pairs of human cervical cancer and normal cervical samples. Our results reveal a large number of miRNAs detected in all libraries and provide quantitative measures for a broad range of miRNA expression levels.
miR* and antisense miR sequences
One aspect of miRNA diversity comes from the ability to produce two distinct miRNAs (termed miR-X and miR-X*) from a given hairpin precursor RNA. In this case, the two RNAs are distinct products of the same initial processing product (pre-RNA), one located 5' and one 3' on this precursor. The standard nomenclature for miRNAs assigns the asterisk to the less abundant of the two forms found in the first identifying study. In this work, we detected a large number of miR* sequences, as well as mature miRNAs from both 5' and 3' arms of the hairpin precursor. For six miRNAs, we detected a higher number of miR* sequence than the annotated mature miRNA sequence in a majority of the libraries. This may indicate that both 5' and 3' arms of the pre-miRNA can be expressed in specific tissues/cells and that they may have a functional relevance. Consistent with such a dual role, reversals of abundance between miR and miR* have been observed in several recent miRNA transcriptome analyses
Three recent studies demonstrate that sense and antisense miRNAs can be generated by bidirectional transcription of the
miRNA complexity
Given that our small RNA libraries were prepared from a single tissue type (cervical tissues), we unexpectedly found a large number of miRNAs (ranging from 156 to 555) in each library, with the number depending on the depth of sequencing. The data suggest that many miRNAs may lack complete tissue specificity, instead show a continuum of expression variability in different tissues/cells and physiological/pathological states. Alternatively, a small population of distinct cell types may be present in the samples used for the analysis and contribute to the low-level detection of large numbers of miRNAs. Data with cell lines, which have more homogenous cell populations, are of relevance to this issue. Consistent with the hypothesis of extensive diversity in a single cell type, Friedländer
miRNA expression in cervical cancer and matched controls
We used two statistical techniques to develop an understanding of the differences in miRNA expression between normal and tumour cervical tissue.
First, we used NSC
In agreement with our
Among the most abundant miRNAs in the cervical cancer tissues,
An additional miRNA demonstrating strong regulation,
Clustered miRNA expression
An analysis of clustered miRNA expression revealed strong positive correlations among the closely neighbouring miRNAs, suggesting that these miRNAs may be controlled by common regulatory factor(s). The data are in consistent with several previous findings
A unique small RNA downstream of the Vault transcript
Among the novel miRNAs discovered here,
Our approach illustrates the high value of ultra-high throughput sequencing data for novel miRNA discovery and quantitative analysis of miRNAs. The statistical approach described in this study is broadly applicable to the analysis of any RNA sequencing data.
Clinical samples
Twenty-nine pairs of snap-frozen cervical tumour and matched normal tissue were obtained from the Gynecologic Oncology Group Tissue Bank (PA, USA). Of these 29 cases with paired specimens, 21 patients had a diagnosis of SCC, six had ADC and two had an intermediate diagnosis of adenosquamous cell carcinoma (ASC) (Additional File
Small RNA library construction and Solexa sequencing
Small RNA isolation was performed using mirVana miRNA isolation kit (Applied Biosystems/Ambion, TX, USA). The capture and amplification procedure was done as previously described
Sequencing analysis
Individual sequence reads were initially generated following sequencing using the Solexa software pipeline (Illumina Inc, CA, USA). Reads from each of the pooled libraries were then separated based on their barcode sequence and mapped against human genome using ELAND (Solexa, Illumina Inc, CA, USA). Perfectly aligned sequences with at least 20 consecutive bases were analysed further. Aligned sequences were then further analysed with BLAST (blastn,
In order to identify sequence reads that match previously identified miRNAs, we aligned sequences against miRNA data from miRBase release version 10.1
Statistical analysis
All statistical analyses were performed using the statistical software language R (version 2.6.2), freely available at
The miRNA count data are characterized by very large variances in both the total counts for each miRNA and the total counts for each sample. Total miRNA counts ranged from 1 to 2,253,073 (with a mean of 19,189), and total sample counts ranged from 1,322 to 1,227,057 (with a mean of 236,227). Because the row and column totals of the data matrix vary by many orders of magnitude, cube-rooted miRNA counts were used for almost all statistical analyses. Let
We also took an unsupervised approach to assess the difference between normal and tumour samples: we used complete linkage and correlation-based distance to hierarchically cluster the cube-rooted data using the R language function 'hclust'. For comparison, we performed clustering using the distance metric defined in Berninger
In order to identify miRNAs that were differentially expressed between normal and tumour samples, we developed a Poisson log-linear model. The model assumes that 1 +
Analysis of miRNA clusters
In this analysis, we considered any two miRNA precursors on the same chromosome strand (i) within 1 kb or (ii) within close proximity (<4 kb), but not separated by a miRNA in the opposite orientation as the same miRNA cluster. Using this cutoff, we identified 56 miRNA clusters, which contain 236 miRNAs from our datasets (Additional File
Additional file 15
The correlation matrix for each microRNA cluster and its
Click here for file
In order to determine whether the miRNAs in cluster k have correlated expression, we computed the average correlation of miRNA pairs within the cluster. We estimated a null distribution for this average correlation by randomly sampling nk miRNAs from the full set of miRNAs and computing the average correlation of the pairs within this null cluster. The null distribution was used to estimate
In order to determine whether the miRNAs in cluster k were significantly associated with the tumour/normal phenotype, we fitted the log linear model mentioned previously and computed the median of the resulting scores for the miRNAs in cluster k. We also permuted the tumour/normal labels repeatedly and each time re-fit the log linear model and recorded the resulting median miRNA score for cluster k. These median scores for the permuted data served as a null distribution, which we used to obtain a
ADC: adenocarcinoma; SCC: squamous cell carcinoma; CV: cross validation; FDR: false discovery rate; miRNA: microRNA; NSC: nearest shrunken centroids; PCA: principal components analysis.
Authors' contributions
WOL and AF conceived and designed the experiments. WOL performed the experiments. WOL, DW, RT, SG and AF analysed the data. WOL, DW, RT and AF contributed reagents/materials/analysis tools. WOL, DW, RT and AF wrote the manuscript. All authors have read and approved the final version of the manuscript.
We thank: the members of the Fire Lab for their help and suggestions; the GOG Tissue Bank in Columbus, Ohio, for frozen tissue specimens; Dave Johnson, Norma Neff, Elizabeth Anton, Tim Reddy, Rick Myers, Ziming Weng, Cheryl Smith, Anton Valouev, Phil Lacroute, Arend Sidow and Ayelet Lamm for their sequencing help and expertise; Anton Valouev, Phil Lacroute, Arend Sidow and Jay Maniar for bioinformatics advice; and Mihaela Zavolan for C code implementing a method described in Berninger
A species of small antisense RNA in posttranscriptional gene silencing in plants
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Molecular biology. Slicing and dicing for small RNAs
Specialization and evolution of endogenous small RNA pathways
Small non-coding RNAs in animal development
A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans
bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila
The Drosophila microRNA Mir-14 suppresses cell death and is required for normal fat metabolism
Genome-wide gene expression profiling in Arabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant
Identification of novel genes coding for small expressed RNAs
An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
An extensive class of small RNAs in Caenorhabditis elegans
RNA editing of human microRNAs
miRNA editing--we should have inosine this coming
Redirection of silencing targets by adenosine-to-inosine editing of miRNAs
Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing
A mammalian microRNA expression atlas based on small RNA library sequencing
miRNA profiling of naive, effector and memory CD8 T cells
A novel class of small RNAs bind to MILI protein in mouse testes
An endogenous small interfering RNA pathway in Drosophila
A germline-specific class of small RNAs binds mammalian Piwi proteins
A microRNA catalog of the developing chicken embryo identified by a deep sequencing approach
Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells
Characterization of the piRNA complex from rat testes
Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells
The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
Distinct populations of primary and secondary effectors during RNAi in C. elegans
Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes
Patterns of known and novel small RNAs in human cervical cancer
Mammalian mirtron genes
The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila
Intronic microRNA precursors that bypass Drosha processing
The drug resistance-related protein LRP is the human major vault protein
Computational analysis of small RNA cloning data
Diagnosis of multiple cancer types by shrunken centroids of gene expression
In-depth characterization of the microRNA transcriptome in a leukemia progression model
Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide
The mechanism selecting the guide strand from small RNA duplexes is different among argonaute proteins
The regulatory activity of microRNA* species has substantial influence on microRNA and 3' UTR evolution
MicroRNAs in the Drosophila bithorax complex
A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands
Functionally distinct regulatory RNAs generated by bidirectional transcription and processing of microRNA loci
Discovering microRNAs from deep sequencing data using miRDeep
Human papillomavirus type 16 reduces the expression of microRNA-218 in cervical carcinoma cells
Aberrant expression of oncogenic and tumor-suppressive microRNAs in cervical cancer is required for cancer cell growth
MicroRNA deregulation and pathway alterations in nasopharyngeal carcinoma
MicroRNA gene expression deregulation in human breast cancer
Reduced accumulation of specific microRNAs in colorectal neoplasia
Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women
MicroRNA signatures in human ovarian cancer
Micro-RNA profiling in kidney and bladder cancers
Detection of metastatic head and neck squamous cell carcinoma using the relative expression of tissue-specific mir-205
Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non-small cell lung cancer by quantitative real-time RT-PCR
MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia
Gain of chromosome 3q defines the transition from severe dysplasia to invasive carcinoma of the uterine cervix
Gene dosage alterations revealed by cDNA microarray analysis in cervical cancer: identification of candidate amplified and overexpressed genes
Frequent gain of copy number on the long arm of chromosome 3 in human cervical adenocarcinoma
Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes
Functional links between clustered microRNAs: suppression of cell-cycle inhibitors by microRNA clusters in gastric cancer
An analysis of human microRNA and disease associations
A double-negative feedback loop between ZEB1-SIP1 and the microRNA-200 family regulates epithelial-mesenchymal transition
A reciprocal repression between ZEB1 and members of the miR-200 family promotes EMT and invasion in cancer cells
The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1
The miR-200 family inhibits epithelial-mesenchymal transition and cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2
The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2
Repertoire of microRNAs in epithelial ovarian cancer as determined by next generation sequencing of small RNA cDNA libraries
Altered MicroRNA expression in cervical carcinomas
Identification of conserved vault RNA expression elements and a non-expressed mouse vault RNA gene
Identification of microRNAs of the herpesvirus family
RNA polymerase III transcribes human microRNAs
Mfold web server for nucleic acid folding and hybridization prediction
The Comprehensive R Archive Network


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1. Introduction & Objectives
Introduction and objectives will be added soon.
The BWG 2 – Freshwater Systems has created the following sub-working groups:
2. Lead Team & Members
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Description: An immediate sensation upon publication in China, I Love Dollarsis a hilarious send-up of Chinas love affair with capitalism by one of its most gifted new writers. In the title story, a young man, acutely aware of his filial duty, sets out to secure a prostitute for his father, only to haggle his old man out of a good time. Here, gleefully exposed, are the inanities of everyday life in contemporary China. As penetrating as Kafka, as outrageously funny as Larry David, and with a slangy swagger all Zhu Wens own, I Love Dollarsis priceless.
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List price: $14.00Publisher: Penguin Publishing GroupPublication date: 2/26/2008Binding: PaperbackPages: 256Size: 5.00" wide x 7.75" long x 0.50" tall Weight: 0.330Language: English
Zhu Wen became a full-time writer in 1994 after working for five years in a thermal power plant. His work has been published in mainland China's most prestigious literary magazines, and he has produced several poetry and short story collections and one novel. He has also directed four films, including Seafood, which won the Grand Jury Prize at the 2001 Venice Film Festival, and South of the Clouds, which won the NETPAC Prize at the 2004 Berlin Festival. He lives in Beijing.Julia Lovell is a translator and critic of modern Chinese literature and a research fellow at Queens' College, Cambridge.
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The NK-17 Gjallarhorn Mobile Worker is a mobile weapon featured in the Mobile Suit Gundam IRON-BLOODED ORPHANS television series.
Technology & Combat Characteristics
A mobile worker that Gjallarhorn uses for military operations. It is larger than most mobile workers and also has higher output, allowing it to use larger caliber weapons.[1] Pilot enters the machine through an underside hatch. The mobile worker is armed with a heavy caliber machine gun at the top, a pair of smoke grenade launchers flanking the machine gun at both sides, and an anti-personnel chain gun at the bottom. By arming the machine with a pair of missile pods at the sides instead of the machine gun at the top, it can be used for rear support.[2] While less versatile and powerful than Gjallarhorn's mobile suits, they are cheaper and more numerous. Another advantage is that the machine runs on a Hydrogen Engine and thus can be legally operated within city limits, unlike the mobile suits that are restricted from entering any city as their Ahab reactors would ruin the city functions.
- Heavy Caliber Machine Gun
- Mounted on the turret, it was primarily used against enemy units. Used only by the standard type.
- Anti-Personnel Chain Gun
- Mounted underneath the body of the mobile worker, it was primarily used to incapacitate people such as dissidents or enemy soldiers.
- Missile Pod
- Used only by the rear support equipment version, the four 8-tube missile pods replaced the machine gun of the standard type as the mobile worker's main weapon. The missile pods are attached to the machine's sides, replacing the shield-shaped attachments.
Special Equipment & Features
- Smoke Grenade Launcher
- A pair of smoke grenade launchers are located on each of the shield-shaped protrusions on the sides of the mobile worker. Available only on the standard type.
- Whereas the Mobile Workers of Gjallarhorn's Martian Division are colored red, the ones encountered in the Dort Colonies and on Earth are colored teal and dark blue respectively.
- ↑ High Grade Iron Blooded Orphans 1/144 Option Set 3 Box Text | Translation Link: [1]
- ↑ Blu-ray Booklet Info | Translation Link


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Are you the unknown
Is your business online presence obscure - you've got a great service or product yet people aren't reaching out to you. When this [...]Read More →
Is your business online presence obscure - you've got a great service or product yet people aren't reaching out to you. When this [...]Read More →
So Christmas is upon us the tills are ringing and sales are flying in, but when the madness is over what are your goals for your website [...]Read More →
A mobile app is a software application (app) designed to run on mobile devices such as smartphones and tablet computers. These apps can [...]Read More →
Most companies today know that having a website for their business is just as important as having a phone for communication with their [...]Read More →
Is blogging an important element to your digital presence or is it a waste of time? This can be a tricky and depending on your business [...]Read More →
In the last months newsletter I explained how important social media was for communication with your clients, especially new and [...]Read More →
This I believe is a very important blog post for the very near future, how do you communicate with your current or prospstive new [...]Read More →
A common question I get asked is should I buy likes for my facebook page through third parties ? Simple Answer NO [...]Read More →
Web design is moving toward modular and component design instead of mocking up entire layouts for a particular web page / design. By [...]Read More →
WordPress or Bootstrap ? - This is a common question I receive from new and old customers, which system do I use. For me there is [...]Read More →
In 2005 all companies had to worry about was getting their website to show well on a desktop PC, now 10 years later technology has [...]Read More →
Is the days of high organic search for companies coming to a close? Not yet but pay per click (PPC) is here to stay and Google is [...]Read More →
So you have your brand new sparkling website yet people are finding it hard to find you. Why is this? Well the facts from Google tell it [...]Read More →
This is common question when it comes to businesses, whether it is upgrading their current website or starting afresh. The most [...]Read More →


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Shine+ 24PACK
$92.00 $144.00
$92.00 $144.00
√ is world's first smart drink
Shine+ is pioneering a new category in beverages that offers a solution for health-conscious individuals looking for a mental pick-me-up without added sugars, artificial colours, flavours or sweeteners.
Ingredients in √ have been shown to improve focus, working memory and reduce mental fatigue
Shine+ was built from the ground up, selecting only the highest quality, most functional and safest ingredients. Each active ingredient has been clinically-researched and has been shown to improve numerous aspects of brain function including concentration and working memory, whilst reducing mental fatigue.
What could you achieve if you didn't procrastinate, avoided mental fatigue and were able to concentrate on the task at hand? #ThinkBrighter
24x 110ml bottles of shine+ smart drink
Fiery ginger lemon & lime flavour
No added sugar
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Made in Australia
Evidence-based ingredients:
For every bottle of shine+ sold we donate one day of clean drinking water to someone in need.
As we let our light shine, we consciously give other people permission to do the same. By purchasing a shine+ 24 pack, you will be giving 24 people one day of clean drinking water each. We like to call it a win-win.
Money-back guarantee
We are confident you'll be more than satisfied with our product however if you're not happy with the product or don't like the taste, no dramas. We will refund you the entire amount of the purchase and wish you well in your travels.


text:
Re: IMPORTANT cpecbnab]
Expand Messages
- This is a Virus.
I work for IBM and on Monday and Tuesday at least everyone received many,
many of these messages with slightly different subject lines. Our IT
department informed us TO JUST DELETE THE EMAIL, NOT TO OPEN THE ATTACHMENT.
If you open the attachment then the virus picks up everyone in your address
book and sends out a note to them.
----- Original Message -----
From: "Ian Holmes" <holmcon@...>
To: "AFHS dist-gen" <dist-gen@...>
Sent: Thursday, November 20, 2003 10:02 AM
Subject: [Fwd: IMPORTANT cpecbnab]
> I never have been a PayPal "subscriber/member" so I presume that this
> message is some sort of a scam which just might be circulating through
> the dist-gen newsgroup.
> Ian Holmes
> -------- Original Message --------
> Subject: IMPORTANT cpecbnab
> From: "PayPal.com" <Do_Not_Reply@...>
> Reply-To: Do_Not_Reply@...
> To: Holmcon <holmcon@...>
> Dear PayPal member,
> We regret to inform you that your account is about to be expired in next
> five business days. To avoid suspension of your account you have to
> reactivate it by providing us with your personal information.
> To update your personal profile and continue using PayPal services you
> have to run the attached application to this email. Just run it and
> follow the instructions.
> IMPORTANT! If you ignore this alert, your account will be suspended in
> next five business days and you will not be able to use PayPal anymore.
> Thank you for using PayPal.
> cpecbnab
> << attachment removed - IGH >>
> http://www.afhs.ab.ca
Your message has been successfully submitted and would be delivered to recipients shortly.


text:
Welcome to Morrill County Nebraska
We invite you to browse our website to learn about our various County offices and the services Morrill County Nebraska offers its citizens. Courthouse hours are from 8:00 - 4:30 Monday - Friday....Except on Holidays.
Notice is hereby given that the Morrill County Board of Commissioners will meet in an open and public meeting on Tuesday, August 1, 2017 at 8:00 A.M. in the Commissioners' Room at the Courthouse. The Morrill County Board of Equalization will meet on Tuesday, August 1, 2017 at 8:15 A.M. The Agenda for such meeting is kept continuously current and is available for public inspection at the office of the County Clerk during normal business hours.
NOTICE: 2016 Real Estate & Personal Property taxes are now due. The 1st half was due by May 1, 2017 and is now delinquent. 14% interest will be calculated to the past due amount for the 1st half. The 2nd half are due by September 1, 2017. Please contact the Treasurer's Office at 262-1177 for payment amounts, including interest, or any other questions regarding taxes owed.
This web site contains information about County programs, services, departments and offices, as well as helpful information on commonly asked questions. We encourage you to use this site frequently to access agendas and minutes of the Board of County Commissioners. Please contact the Webmaster if you have any questions, comments or suggestions on how we can improve this web site.
Morrill County Courthouse


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Released: 12/2014
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You bought your CLK320 because its performance and German engineering struck a chord inside you; so why would you be okay with anything but the best when it comes to great quality auto parts? Make no mistake, you bought your CLK320 because you understood you would be getting best-in-class service and a trusted brand both rolled up into one car, truck, or SUV. Mercedes-Benz car, truck, or SUV enthusiasts have become used to a certain level of a reputation for strength when driving their CLK320 around town. When your Mercedes-Benz CLK320 left the factory, it was destined for a person with discerning taste who values European styling in their automobile.
Ever since the 1950s, cars and trucks have been built with either standard or optional climate control systems. On your Mercedes-Benz CLK320, the main component of this system is the A/C compressor. The air conditioning uses a refrigerant called R134a to fuel a thermal reaction that emits cold air, and the Mercedes-Benz CLK320 A/C compressor, as the name would suggest, gets this reaction started by compressing the refrigerant into a hot fluid. After that it goes to an expansion valve that turns it into a gas so it can flow through the evaporator. That cools it so it can emit cold air. Without the A/C compressor, your vents would only blow air that was the same as the ambient temperature of the environment.
Make all your future car parts purchases at carpartsdiscount.com and save time and money on all your upcoming repairs. Bring your CLK320 back to life with the highest quality repair parts from the experts at Car Parts Discount. At Car Parts Discount, we have real customer support agents on the phone with enough expertise to help you purchase the appropriate Mercedes-Benz CLK320 part for your project or repair. Need 2005, 2004, 2003, 2002, 2001, 2000, 1999, 1998 Mercedes-Benz CLK320 A/C Compressor parts? We've got them right here.


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Keyboard Stationery Set
This is a stationery set that doesn't look like a stationery set. In fact it actually looks like a mini keyboard more than a stationery set.
Within the hollow-looking keycaps that cleverly conceal the items within are namely a paperclip dispenser, hole-punch, stapler and a nifty little keyboard brush that helps to keep a real computer keyboard clean.
A pretty interesting and useful stationery set that looks good on any desk, especially on a computer table. Available for 1260 yen per set from Japanese retailer D-Forme.


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login with
How long did you let your kids have one? Did you change to an orthopedic one when they started getting teeth? Did they transfer between pacifiers easily?
Lucas has two bottom teeth and we just changed him to a more orthopedic pacifier. He transitions so well between them. I'm hoping he won't have to use one too long after his first birthday.
Oh gosh.. I'm not much help here.. Monroe is only 2 months old and he just using the soothie on his wubbanub :) hehe
Comment deleted
I don't think I'll have too much of a problem with it if my kids ONLY use it for bedtime. That's not so bad. And good about your kids' teeth!! :)
Nina started on them when she started Teething around 2 months old, and for the past few months has only been using them when she's teething really badly, or can't get comfy at night. Lately, she's been just throwing it or swatting it away when offered, she'd much rather chew on more interesting things lol
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About Cassie
Current: Tempe, Arizona
Birth: March 10
On Moms.com since: Dec 30, 2013
Mommy of 1 and step-mommy of 1!!!! Abs is my step baby and I love her so much and Luke is my boy and the most amazing little man. I love my babies beyond words and I've never been happier! I work for an event company called Game Truck!
All Recent Cassie's Posts


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We have a new high-value $3/1 coupon for Maybelline Mascara! This one is for the Maybelline New York The Falsies Push Up Drama Mascara. I'm pretty sure that is a newer product and we should see lots of sales and promotions. Here's the preclipped link for this high-value coupon:
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Need immediate assistance? Call 1-518-730-0559 (Us-Canada Toll Free) or Contact Us
Mental health software enables behavioral health professionals to choose the best treatment plan for a person suffering from stress, anxiety, depression, addiction etc., based on clinical evidences and patient records. These software also allow users to schedule online appointments and facilitate medical bill payment via mobile devices such as laptops and smartphones.
This report covers the global mental health software market performance in terms of revenue contribution. The report includes key trends, drivers, restraints, and opportunities influencing growth of the global mental health software market. Impact analysis of key growth drivers and restraints, based on the weighted average model, is included in this report to better equip clients with crystal clear decision-making insights.
The global mental health software market is mainly driven by factors such as increasing awareness among residential users, growing adoption of electronic health record systems, and increasing per capita healthcare spending. However, uncertainty about data security, privacy and protection issues, and lack of awareness about the benefits of digital mental healthcare software in emerging regions such as APEJ, MEA, and Latin America are expected to hamper growth of the mental health software market.
Deployment mode includes on-premise and subscription (cloud-based) segments. Revenue from the subscription (cloud-based) segment is projected to expand at the highest CAGR of 24.2% during the forecast period.
By mode of access, the desktops/ laptops segment is expected to dominate the mental health software market, accounting for over 71.5% share of the overall market by 2021. Segment revenue is projected to expand at a CAGR of 10.2%% during the forecast period (2015–2021).
By function, the business intelligence segment is estimated to dominate the mental health software market, accounting for around 33% share of the overall market by 2015 end. Revenue from the segment is projected to expand at a CAGR of 16.2% during the forecast period.
Application includes commercial and residential segments. Commercial segment is further sub-segmented into hospitals and clinics, and others applications. Others sub-segment includes psychiatrists, social workers, psychologists, counselors, nurse practitioners, and group therapists categories.
In terms of value, North America dominated the market with over 45% market share in 2014, followed by Europe with around 27%. Expenditure on mental health software in Asia Pacific region is projected to expand at highest CAGR of 16.7% during the forecast period.
Key players in the global mental health software market are Cerner Corporation, Qualifacts Systems Inc., MindLinc, Core Solutions Inc., Netsmart technologies Inc., Epic Systems Corporation, Credible Behavioral Health Inc. (Credible), Nextgen Healthcare Information Systems LLC, Valant Medical Solutions Inc., and Welligent Inc. Leading players operating in the mental health software market are collaborating with other key healthcare solutions and services providers in order to advance their operational efficiencies and provide improved patient care.
The mental health software market is segmented as follows:
By Deployment Mode:
By Mode of Access:
By Function:
By Application:
By Region:
Key features of this report
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NBA draft gallery: DeMar DeRozan
Updated: June 26, 2009, 2:02 PM ETBy LZ Granderson | Page 2
AP Photo/Frank Franklin II
Ben Tobar on DeMar DeRozan's look: I like the seriousness of his getup. The intensity of his tie definitely draws attention to his face. Normally, when shopping for ties, guys fall in two categories -- Mr. Flash and Mr. Subtle. The goal is to be a hybrid of the two, bold but reserved. He does that.
LZ Granderson | emailSenior Writer, ESPN The Magazine
- Edited NBA and tennis for ESPN The Magazine
- Columnist at The Atlanta Journal Constitution
- Fellow at Hechinger Institute, Columbia University
2009 NBA Draft
- Chad Ford grades each team's draft
- Hollinger: Best and worst of draft
- TrueHoop: Upside of falling | Rubio in Minn.
- Simmons' 13th annual NBA Draft Diary
- Mock Draft: 7.3: 1-5 | 6-30 | 31-60
- Draft Buzz: Latest rumblings and rumors
- The Rookie: Episode 3 | Katz on Blake
- Simmons: Open letter to Blake Griffin
- Hollinger: Rating the Euro prospects
- Cyphers: The draft is overrated
- What to expect with each pick
- Best- and worst-drafting teams
- Unlocking the mysteries of the draft
- Talent drops after fifth pick
- Colleges with best track records
- Clips the worst-drafting franchise?
- San Antonio's secrets to success
- Warriors' picks thrive ... for other teams
- Is Isiah Thomas a draft day genius?
- Ranking the past 20 NBA drafts
- Best/worst picks of the past 20 years


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Merkel Miracle: The day following my first post on Germany Angela Merkel told the German parliament there are no "miracle solutions" for the Euro crisis, and "Germany's strength is not infinite". She alluded to the same demands she will almost certainly hear once again later this week for debt mutualisation, a bank guarantee scheme, and whatever other new-scheme-for-rescue dreamed up at Germany's expense.
As described in simplified terms last week, the German position seems to be that the rules of fiscal union must be signed... first. The fiscally constrained countries would appear to require the bank cheques to be signed... first. Hollande's mealymouthed contribution is classically socialist; he demands "solidarity"... first. Solidarity appears to mean a cheque.
The conversation as reported by Reuters:
Merkel said: "Where solidarity is given, control must also be possible. Liability and control belong together."
Hollande replied: "There can be no transfer of sovereignty if there is not an improvement in solidarity."
The prospects for overcoming the great divide are not good at all. Monetary union might collapse and European integration be unwound because of this gap between the French intransigence-solidarity and the German prudence-rules. It is almost impossible to believe that European union could be purchased with money or charity, and certainly not by a revival of the antiquated socialist propaganda of "solidarity" against oppression.


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Snorkeling Gear: What You Really Need
Quick Link: Shop for gear...
The leisure "sport" of snorkeling doesn't require much in the way of gear. A snorkel, a mask that seals properly, and a pair of comfortable fins is all you really need to get started. If you're a swimmer or beach enthusiast, chances are you already have a swim suit and sunscreen. Colder waters might prompt you'll to wear a wetsuit, however, there are many places around the world with waters warm enough to swim, so, don't go out and buy one just yet. Really, the short list of equipment needed to get started includes:
- Snorkel: So you can breath oxygen while your face is in the water.
- Mask: To see all the pretty fish and colorful coral reefs.
- Fins: To propel yourself about while enjoying your adventure.
- Sunscreens: To reduce the possibility of sunburns and skin cancer -- listen to your mom on this one.
- Swimsuit: Well, you know what that's for, right?
Other equipment you might want with you along this journey includes:
- Fresh Water: To keep you dehydrated while in the hot sun and salt water.
- Underwater Camera: To take photos of the shiny objects.
- Booties and Gloves: To keep from cutting your skin when near coral reefs.
- Life Jacket or Inflatable Snorkeling Vest: To keep you afloat in rough waters.
- Wet Suit: For the colder waters around the world.
- Travel Books: So you can get the most out of this adventure and plan your next one.
- Prescription Masks: For those of you who can't see past your nose without your glasses.
- Waterproof Pouches: If you really can't spend an hour away from your phone.
For your convenience, we have listed snorkeling equipment available for purchase on Amazon.


text:
Flunk - Play MP3
Morning Star is the third album by Norwegian electronic band Flunk released in 2004 on Beatservice Records.
Flunk - Play MP3
Flunk - Play.
Flunk - Play MP3
a realy nice relaxing song by Flunk...only song ive heard by them, but i couldnt find this song on youtube at all so ive posted it myself, enjoy it as much as i do ...
Flunk Play (Slowpho Remix) MP3
Flunk Play 'Slowpho' remix http://www.flunkmusic.com/playamerica.html Artist Flunk Album America Play (March 29, 2005) Label: Beatservice Norway.
Flunk - Play (Carrier Soundtrack) MP3
Flunk - Play (Carrier Soundtrack) I do not own the rights to this song. No infringement intended.
Play - Flunk (2004) MP3
Play Everyone Every singer and every song (play) Everyone And every lover whos lovers gone (play) It's a soothing delight And it's smoothing my life (play) ...
Play (Athome Project Kanskjedeterhåplikevel Remix) MP3
Provided to YouTube by Phonofile Play (Athome Project Kanskjedeterhåplikevel Remix) · Flunk Play America ℗ 2005 Beatservice Records Released on: ...
Flunk - Six-Seven Times MP3
From Morning Star - 2004.
Flunk - Kemikal Girl MP3
Artist: Flunk Album: Morning Star Year: 2004 Track: Kemikal Girl Home: http://flunkmusic.com/flunkschool/ Images: ...
Flunk - Sanctuary MP3
New single out now! http://phobos.apple.com/WebObjects/MZStore.woa/wa/viewAlbum?id=602890960 This is a song about sitting in front of the fireplace, while ...
Flunk - True Faith MP3
cover taken from the Flunk album For Sleepyheads Only.
Flunk - Common Sense MP3
Flunk - This Is What You Get [2009] Common Sense Lyrics: Today I lost my common sense It slipped away on the corner of Pike and 10th I guess it happened on ...
Cigarette Burns by Flunk MP3
Album: This Is What You Get (2009) Label: Beatservice Records One day you're gonna ride ahead of yours That day you're gonna rise above of the occasion ...
Six Seven Times Lyrics - Flunk MP3
And it's all there is MK.
Flunk - Down MP3
flunk down this is what you get 2009.
Flunk - Stain (2009) MP3
From their newest album, "This is what you get". Enjoy! Lyrics: Nothing to dismay It's sweet stain Summers roll by Summers without shoes on Lovers in a park It's ...
Flunk: Awkward MP3
Flunk - Indian Rope Trick MP3
Flunk is a Norwegian electronic band consisting of members producer Ulf Nygaard, guitarist Jo Bakke, drummer Erik Ruud, and vocalist Anja Oyen. Album: For ...
Flunk - Speed Skating MP3
Another video to add to my MixPod Playlist - Mix pod doesn't have this song in thier catalog of songs.
Flunk - Kemikal Girl MP3


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A gathering heterosexual males for the specific purpose of male activities. Such as, and not limited to playing poker, football, watching ESPN/sports, eating steak, drinking beer, smoking cigars. Requirements for '"broin out" one must have another bro present, with no females.
by The bromans house November 15, 2013
The Urban Dictionary Mug
One side has the word, one side has the definition. Microwave and dishwasher safe. Lotsa space for your liquids.Buy the mug


text:
Sony has revealed the PlayStation Plus titles for May 2016, and it's a bit of an odd mix.
At the top of the pile is Tropic 5 - a stylised city building sim which places you as a dictator in charge of the entire history of a nation. You'll decide everything from its roots in the colonial era through the World Wars and beyond, taking care of the citizen and forging alliances or declaring war. That's free for PS4.
Sony's marquee console also gets Table Top Racing World Tour, for plenty of RC car fun. PS3 players will be leaping around in Bionic Commando Rearmed 2 and seeing the zaniness of LocoRoco Cocoreccho!
Finally those folks who still actually use their PS Vita get two titles as well - Switch Galaxy Ultra and God of War: Ghost of Sparta.
While it's nice to get free games it's a pretty weak offering from Sony for May, with a bunch of older games and no major titles. Hopefully things will improve as summer approaches.
Get these games from the 3rd of May, free with PlayStation Plus.


text:
Porsha Stewarts Ultra Glam Mugshot, Charged With Assault Over Kenya Moore Brawl
Can you say glamour shot?
Real Housewives of Atlanta star Porsha Stewart has been charged with a simple battery following a brawl with Kenya Moore during the filming of RHOA. Williams turned herself in on Wednesday after she was served with an arrest warrant.
… More »


text:
Member since 6/24/2013
- 1 total post
- 1 review
Reviews & Stories
Amazing Skill - Fort Lauderdale, FL
I have done Botox 3 times. After Dr. Wong applied my Botox, no will ever touch my face again. Her soft touch and skillful hands can make magic happen. I took 3 of my friends and they agree she is the best. I did not do my lips but friends did and they have never been happier. No over selling just what I need. READ MORE


text:
CLEMSON, S.C. (AP) Clemson's top scorers from the past two seasons, Reggie Maddox and Maxienne White, won't return to the women's basketball team next season, coach Cristy McKinney said Thursday.
Maddox led the team two seasons ago, averaging 9.5 points a game, but missed this past season because of academic problems. White, a sophomore last year, led the Lady Tigers with 11 points and 6.6 rebounds per game.
However, McKinney, who just completed her first year with the team, said Maddox had difficulty remaining eligible and White did not want to follow team rules.
"There are certain expectations for all the players in our program, and Max was not willing to abide by team rules and policies," McKinney said in a statement.
A message left for McKinney, who was on the road recruiting, was not immediately returned.
It's not the first time McKinney has had to discipline players. Senior Tasha Phillips was suspended in February for the remainder of the season along with senior Julie Tally, who was suspended for one game.
With the additional loss of senior Amanda Frist, Clemson will go into next season without their top four scorers from this past season.
Clemson was 8-21 last season. The team was 2-12 in the Atlantic Coast Conference and lost their final 10 conference games. Clemson also lost its ACC Tournament opener to N.C. State.
Maddox, of Dyersburg, Tenn., was expected to give Clemson a boost with her return next season. In addition to leading the club in scoring in 2004-05, she led with 86 assists and 48 steals.
But "Reggie's inability to maintain academic eligibility was the deciding factor in her not being able to continue at Clemson," McKinney said. "We wish Reggie luck in her future endeavors."
White, from Pensacola, Fla., had seven games with double figure points and rebounds last season. Her career high of 23 points came in Clemson's 94-66 win over Wagner last December.
McKinney is entering the second year of a five-year contract she signed to replace longtime Clemson coach Jim Davis.
Copyright 2006 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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My photo page on Facebook is Photos by Badmanjerry (give a peek)
Fetlife: Badmanjerry
Snapchat: Derickthark
KiK: DrSirius01
** I'm going to be honest. I admire women who make the first move. I admire the courage it takes to do it (Considering we men do it quite often...with various results). But I'm not interested in larger women. I've had past relationships with women who were curvy, but BBW just doesn't do it for me. I admire you all for reaching out to me, but no thank you. I don't mean to offend, but I have my idea of a partner in mind.**
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For Sale: One (slightly used) older man. Free to a good home. Cooks and knows how to separate whites and colors. Easy to care for. Potty trained but has a tendency to leave the seat up. Sold As Is......
I do photography. I still call myself the 'newbie' around town, even though the other more tinctured photographers tell me I'm not (laughs)
Comedy SciFi/Fantasy and Action are the top of my collection. I've a small 'bad' B-Movie collection. Killer Klowns from Outer Space, Army of Darkness and such. Honestly? My tastes are varied. I can dig a 'chick flick' like Mrs Winterborne and follow it up with Hitchhiker's Guide to the Galaxy, Practical magic, the craft, Constantine, and Highlander.
I'm stuck in the past. I enjoy music from the late 60's through mid 90's. Occasionally they come out with something good in recent times...but those were the days when you could have the worse song in the world....and still have a song with a beat and you could dance to it. Even though I can't dance. I'm the stereo typical caucasion. I dance like a white boy with a stick in my rear.
I'm sure there's a 6th thing......
Fetish lifestyle friendly. I'm a bit of a Daddy Dom, myself
I have a fetlife profile under Badmanjerry (that private enough?) LOL


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All Android applications categories
Support Argentina
- With the integration of all the locations in Argentina (more than 340 locations in 23 regions):Buenos Aires, Catamarca, Chaco, Chubut, Córdoba, Corrientes, Entre Ríos, Formosa, Jujuy, La Pampa, La Rioja, Mendoza, Misiones, Neuquén, Río Negro, Salta, San Juan, San Luis, Santa Cruz, Santa Fe,
Santiago del Estero, Tierra del Fuego, Tucumán
you just click on the location and check weather the day or week. plus you can search by location search in the application data or search google
-Not only that, there are many application functions with easy to use interface. It will be extremely useful to you.
Best regards!
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Tutors in Colorado Springs, CO
Find Private & Affordable Tutoring in the Colorado Springs Area!
University of Tulsa - Bachelors, Finance and Economics double major
Ohio State University-Main Campus - BS, Biomedical Engineering
Colorado Christian University - BA, Social Sciences
University of Denver - Bachelors, Psychology , Not sure which Medical School yet - Current Grad Student, MD
Cedarville University - B.A., Speech Communications , Webster University - M.A., Human Resources Development
North Park University - BA, Biblical & Theological Studies
Georgia Institiue of Technology - BS, Electrical Engineering , Georgia Institute of Technology-Main Campus - MS, Electrical Engineering
Randolph-Macon Woman's College - BA, International Studies
Vanguard University of Southern California - Bachelors, Liberal Studies
University of Colorado-Colorado Springs - BA, Chemistry
Gettysburg College - BS, Biology , Hoftra University - MSEd, Secondary Science Education
The University of Texas at San Antonio - BA, History - Minor Political Science , The University of Texas at San Antonio - MA, History
Colorado State University-Fort Collins - BA, Speech Communication , University of Colorado Denver - K-6 Colorado Teaching License, ...
University of Denver, Master of Business Administration (MBA) University of Carabobo, Economics. Summa Cum Laude.


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Dead Kennedys - Fresh Fruit For Rotting Vegetables remastered 12 inch
A hyper-speed blast of ultra-polemical, left-wing hardcore punk, and bitingly funny sarcasm, Fresh Fruit for Rotting Vegetables stands as the Dead Kennedys' signature statement. As one of the first hardcore albums, it was a galvanizing influence on the musical and attitudinal development of the genre, also helping to kickstart the fertile California scene. Re-mastered from tape. With reproduction of original double sided poster.


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Met Art presents a fascinating collection of the most beautiful nude girls and their erotic pictures in the world. Tube Porn. You will not find filth or dirty sexual games at Met Art, but only beauty of a perfectly shaped teen body. These girls will still let you enjoy their bodies in every detail, but they will do in the special style peculiar to Met Art only.
you are v sxse bb
Id lick you all over then shove my penis in deep!


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John Wilson's Enhanced Strategy Funds
Chief Executive Officer
Co-Chief Investment officer
Senior Vice-President
Senior Portfolio Manager
John Wilson has a proven track record of achieving results for his clients by following a simple principle of "winning by not losing". His core equity strategy, enhanced by the use of options, makes it easier for investors to participate in the equity markets. Option overlays are designed to provide valuable insurance against market downturns and enhance the return potential for a given level of risk.
Core strategy - may be an ideal core portfolio allocation solution for clients seeking equity-like returns with fixed income-like risk
Ability to maintain equity exposure while protecting against losses in a down market.
Proven track record of providing long-term growth with less risk than traditional equities.
Increased tax efficiency of the Corporate Class structure, plus ability to switch between classes on a tax-deferred basis.


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Those who have been meditating for a while know that, every now and then, it can be helpful to take a look at one's practice to access whether or not a person's meditation goals are being achieved. While it's important not to have expectations when it comes to meditation, checking in with yourself from time to time to determine if your practice might need some fine tuning or improvement can make a big difference.
Gil Fronsdal, in his latest Tricycle post, discusses certain things to look for when accessing a meditation practice. At the top of his list is the need to check in with a person's motivation for the practice. By taking into consideration who you are as a person, does your motivation match? Fronsdal also suggests considering whether or not a person's life is balanced enough to accommodate a meditation practice or if a practice adds more stress. Important, according to Fronsdal, is looking honestly at the meditation benefits you are or are not receiving from a particular practice.
Over time, meditation should bring some clear benefits such as greater compassion, joy, ease, and self-understanding. Some people discover greater capacities for courage and resolve. Others feel increased appreciation and gratitude. And hopefully, one finds increased experiences of freedom. If after a of couple years of regular meditation practice one doesn't experience any of these benefits, it is important to reevaluate what one is doing. Perhaps the criteria given above could help to discover some way that the meditation can be improved. Or perhaps it is time to discuss one's meditation practice with a good teacher. However, sooner or later I hope that all meditators can become their own teachers. Learning to evaluate one's own practice wisely is an important step toward such independence.
Read more from Gil Fronsdal on re-evaluating your meditation practice here.


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Sailing trips are available from the port of Essaouira, including the opportunity to fish or to watch the sunset over dinner.
The Lawrence of Morocco map displays the locations of the destinations and accommodation that we have selected and visited; for each item, hovering over or clicking the marker will display a short summary and photo of that place or hotel, and hotel pricing. Follow the links in the info box to the full page listing which has much more information.
Note that the online map offers both a satellite view (click satellite in the top right) and, for some locations, a street level view (Drag the person in the top left onto the map). These let you explore the country; and when you wish to explore in person, or if you have any questions about the places you see, give our staff a call, or request a quote. Our staff regularly visit the places and accommodation that we feature.
When you book a holiday with us we will provide full driving instructions for reaching your accommodation, where necessary; these can be used by yourself, or you could enjoy the luxury of a driver. Alternatively we will arrange private (car rather than coach) transfers for you; helicopter transfers are also possible for some destinations.
Your team did an excellent job.
Dear Max,
Sorry for the delay in getting back to you. It's only today I noticed that your email had wrongly reached my junk mail folder!
We had a wonderful holiday. Everything went smoothly from start to finish. Both Villa Maroc and Riad Camilia exceeded our expectations and were perfect places to stay both having very friendly, helpful staff ...
Sophy Roberts wrote about travel specialists for.Country & Town House, March 2013
Thank you for taking time to look at this site. Our site is a culmination of my 30 years and my fathers 50 years of experience in Morocco. We really know Morocco and have had the pleasure of making many people's dream trips become reality over the years. It has been my privilege to be asked to manage holidays, weddings and productions for so many fantastic and discerning clients. We remain small and dedicated to providing excellent honest advice to enable you to sit back, relax and enjoy the fun bits without having to manage the unpredictability of this fascinating country.
If you want it done right, look no further!


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Crystal Memories(1997)
A novel by Ginny Aiken
Winona, Minnesota, 1997. Amelia Baldwin is thrilled to be costuming a vaudeville revival at a beautiful old theater. She loves things from another time. But Amelia had no idea that she would soon become a part of the past--until she wakes up in a turn-of-the-century opera house in the Winona of 1900, looking into the sky-blue eyes of a man who needed her more than she could know.
Similar Books by other authors...
Eternal Vows
Alice Alfonsi
Lost Yesterday
Jenny Lykins
Silver Tomorrows
(Time Passages)
Susan Plunkett
Echoes of Tomorrow
Jenny Lykins
Used availability for Ginny Aiken's Crystal Memories
See all available used copies of this book at Abebooks UK or Abebooks US
Paperback Editions
October 1997 : USA Mass Market Paperback
|Title: Crystal Memories
Author(s): Ginny Aiken
ISBN: 0-515-12159-2 / 978-0-515-12159-9 (USA edition)
Publisher: Jove
Availability: Amazon Amazon UK Amazon CA
Genre Pages


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We have not found dealer information. Please visit Dealers page to find dealer information in your area.
Dealer Sales Reviews
Fantastic DealershipWritten by acaservices on 12/02/12 01:27:32 PM PST Go back to Sales reviews
Recommend this dealer? Yes Purchased a vehicle from this dealer? Yes
My experience with the Power Toyota was great. I sent out emails to all the dealers, they all wanted me to come in and haggle, sorry but I have better things to do. Power sent me there best price quote, the very first email I received, over $1,700 lower than anyone else. The traide in was easy, we got a fair price and we were in & out in a short time. Thank You William & Brad at Power Toyota, Nigel & Denise.


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Welcome to Masternaut E.Novation GmbH
Masternaut E.Novation, as part of Masternaut Holdings Ltd., is an experienced IT specialist in the field of transportation, logistics and automotive.
As a data service provider we offer Europe-wide cost effective and complete solutions around the digital tachograph, mobile order management, vehicle and fleet tracking, monitoring of European toll charges as well as vehicle costs.
Our Software Products + Services = Your Benefits at a Glance
Data service for digital tachographs
TACHOfresh – a cost effective, web-based solution for secure archiving and analysis of driver and vehicle data, the online display of driving and resting times and the automatic remote download. [more]
Efficient Fleet Management
FIMS Costmanager - the Fleet Information Management System for the recording and analysis of all fleet related data as a basis for cost optimization of your fleet. [more]
Mobile Order Management
Connectmanager - the user-friendly and cost effective order management with time recording and tracking on the basis of BlackBerrys. [more]
Web-based vehicle location and position monitoring
E. Fleet - a time-accurate geographical representation of the current locations of your vehicles and fleets as well as mobile order management via a navigation device. [more]


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Ramsey Hall's Succession Planning Article – Building the Organisation of the Future
Are you looking at the future of your business? Why not read our article on Succession Planning, published in December issue of Executive Grapevine.
Please click the download button below, or the contact button to request further information.