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that it appears to be a brittle fracture associated with old Royalex.
What someone might do is post a picture of a Royalex boat that was wrapped when it was no more than fiver years old.
When a relatively new Royalex boat pitons hard into a rock, the bow will crumple in relatively smooth folds. I saw a 20+ year old Royalex boat hit an obstacle at high speed, and what resulted was a jagged split.
If we conclude that the canoe under present discussion has gotten brittle, then we'll know that it can't be used in whitewater. It might be repaired and used for lake duty, though the repair may add enough weight to be felt.
Let's leave the repair cloth issue aside until we know what sort of goal we're setting.
P.S. "Yankee" might mean that you are in Bew England. Kaz of millbrookboats.com might have knowledge about the boat's repairability, and might repair it for a reasonable amount, if you and he agree on the money and effort.
but I share the same concerns about the ABS being brittle that others have, and would be reluctant to use it on whitewater.
I would use G Flex epoxy rather than a methyl-methacrylate like Plexus simply because it is much cheaper. It bonds to ABS well. Mixed with a thickening agent like colloidal silica, it can be used to fill voids and fair uneven surfaces on the hull. It also wets out fiberglass and aramid cloth well.
I would try to fit the pieces of broken Royalex together like a jig saw puzzle. Try to secure a stiff piece of cardboard covered with Saran Wrap to the outside of the broken hull to maintain shape. Clear plastic packing tape can also be used to hold the approximated pieces together. Thicken the epoxy with colloidal silica to bond the broken pieces together from the inside and use multiple applications to fair the broken hull surface. G Flex can be mixed up in very small batches and sands well when cured.
When you get the interior as smooth as possible do the same thing on the hull exterior and fair it as best you can. I would apply a single large sheet of aramid to the inside of the hull extending at least 2" beyond all broken edges. You might want to use peel ply over the aramid to get a smoother edge. On the outside I would use a single layer of S 'glass again extending at least 2" beyond the breaks, but try not to have the edges of the fiberglass line up with those of the aramid.
You could use more layers, but I suspect this repair would be as strong or stronger than the remainder of the hull. If you wanted you could cut strips of S 'glass to bond over the joined breaks and feather the edges of those strips before covering the whole with a large sheet of S 'glass.
Feather the edges of the S 'glass well and fill the weave with enough applications of epoxy. Paint it over with red Krylon Fusion spray paint, and if desired, you can spray over the aramid with some flat grey spray paint as well.
You can buy a 32 ounce kit of G Flex epoxy from Sweet Composites for $56 and I think it would be enough to do the repair: http://sweetcomposites.com/WEST.html
Three ounces of aerosil (colloidal silica) will set you back another $4. A piece of 5 ounce per yard aramid 36" x 58" would cost another $27: http://sweetcomposites.com/Kevlar.html
and a piece of 6 oz/yd S 'glass 36" x 60" would be another $19.50: http://sweetcomposites.com/Fiberglass.html
A 36" x 64" piece of nylon fabric (peel ply) would cost $9.50: http://sweetcomposites.com/Polyester.html
So far you are up to $116 less shipping. Adding costs for incidental supplies like disposable gloves, sandpaper, tape, spray paint and shipping costs will put you around $150 or so. To me that would beat taking the boat to the landfill.
I suspect Royalex is prone to these types of puncture holes. I repaired one once that had a softball size puncture. A white pine tree limb fell vertically and punched right through the canoe.
I glued the remaining Royalex puzzle pieces back together with G-flex. I filled any remaining missing puzzle piece areas with thickened G-flex,and then patched the inside with fiberglass and patched the outside with fiberglass. I filled the fiberglass weave with G-flex, sanded and painted with Krylon to protect the epoxy.
As far as I know, the repair has held up fine, but the family uses the canoe for flatwater trips pretty much exclusively.
The hole was smaller than yours and not on the turn of the bilge, which may complicate things.
Good luck.
Thanks for all the tips and war stories, folks.
After getting lots of good points of view from both the "repair it" and "it's toast" camps I've decided to give the repair a go. I did take some good whacks on the hull with a sledge hammer, which bounced off each time (thankfully!). I've decided it's worth repairing it and seeing what happens.
So far, I've beveled (using a dremel grinder and random orbit sander with 80-grit)the edges of the cut and put the first layer of epoxy on the inside to stabilize the hole. I peeled the wax paper off the first side after one day so I could add a second layer of GFlex to a few areas that didn't get enough the first time. I left the wax paper on the outside while I was doing the second coat. When I went to peel the wax off from the outside, much of was stuck to the epoxy that seeped through the cracks. I have had good luck peeling the wax paper away from GFlex in the past (and during this repair from the inside). I'm not sure what happened this time. I guess I'll have to sand it off because if I use a chisel, I'm afraid I'll take off to much of the GFlex that is doing what it's supposed to do.
Anyway, the repair is pretty solid so far. Now I plan to use more GFlex on the outside of the hull all along the crack to finish stabilizing the hull in preparation for the patches. I've ordered kevlar and s-glass material for the inside and outside patches, respectively.
I'll post more pictures once I do the patches. In the meantime, here are a few of the crack stabilizing GFlex process:
More tips are still welcome!
G Flex is more viscous than other epoxies such as System 3, MAS, or West System 105/205-206. I have found that it wets out 6 oz/yd fiberglass or aramid cloth just fine as long as you are applying only one layer at a time and the working temperature is not too cool. The trick is to be patient and let the cloth take its time taking up the epoxy. Don't be in a rush to force it into the weave. Fortunately, G Flex has a pot life of about 45 minutes at room temperature (longer at cooler temperatures) and an even longer working life so you have time.
Yes, you can warm G Flex and it will reduce the viscosity but it will also reduce the pot life and working life so I would try a couple of other things first. If you have an accessory lamp on a cord put a 100 Watt bulb in it and lay it or hang it under the part of the hull you are working on several hours before you apply your cloth. You might be able to put your space heater underneath the inverted hull if there is a good bit of space between it and the Royalex. Obviously take care to avoid overheating the hull. You can also use a hair drier or heat gun to gently warm the part of the hull you are applying cloth to before you put the cloth on the boat and wet it out.
Fiberglass cuts easily with regular scissors. Aramid does not. I have been successful in cutting aramid cloth with scissors but it does dull the scissors and gives your hand a work out. Some people lay the cloth on a cutting board and use a razor knife to cut it.
All woven cloth will tend to fray as you cut it and wet it out. You will get better at minimizing the fraying as you gain experience. If you are cutting rectangular patches on the warp (along the line of the fibers) be careful to cut exactly along the line of one of the glass fibers to minimize the number of transected fibers that will be susceptible to fraying. Patches cut on the bias actually are less prone to fraying along the edges.
Like a lot of folks, I will cut my patches slightly larger than I want the end result to be and then remove a couple of strands from each side before applying it knowing that they will probably fray anyway. The fibers that are left "sticking out" from the patch are easily sanded off after the epoxy is cured.
As you wet out the cloth start applying your epoxy in the center of the patch and rather slowly progress out to the edges. As you apply epoxy to the cut edge very gently dab it on without dislodging the fibers along the edge and let the fibers take up the epoxy before working that part of the cloth very much. Try not to work the cloth in a direction that separates fibers from the edges of the cloth until the epoxy has become tacky enough to hold the cloth down.
You might find that an inexpensive, disposable, metal handle "acid flux" brush is a good tool for applying the epoxy to the cloth.
It generally takes at least 2, and often 3 applications of epoxy to completely fill the weave of the cloth. With each successive application the cloth will take up progressively less epoxy, however. You might not need to completely fill the weave of the aramid cloth on the inside but you will want to do so on the exterior if you want a smooth surface. At cool temperatures, G Flex may take longer than overnight to cure. If you let the epoxy fully cure before applying another coat then you should either wet sand the cured epoxy or wash and rinse it to remove the amine blush which could interfere with bonding of the fresh epoxy.
I will often feather my patches after the wetting out process before filling the weave of the cloth. As long as the epoxy is cured, or nearly cured, you won't disrupt any fibers in the cloth as long as it was properly wet out. I usually do this by wet sanding with water proof paper using something like 180 or 220 grit. After the cloth weave is completely filled I wet sand again down to maybe 400 grit (or finer).
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subset: commoncrawl


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[new] big big planet download [QJ9z] file now
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text:
My Japanese Tree Peony is in full bloom right now and the scent is heavenly. I love the deep red colors with the huge yellow contrasting centers of this beautiful perennial.
Panasonic Lumix DMC ZS1
japanese tree peony peony tree japanese flowers red
Beautiful Vickie
many thanks Carol
Lovely shot Vickie!
thank you Chris
beautiful vickie!
thank you Sharon
Sis, WOW this is just a gorgeous bloom and I am soo jealous.It is still cooler here than there and although the peonies are budding there are no blooms yet. We brought the one you gave me from Regina and have planted it here in the bed at the back of the house. It is growing but we will not see blooms from it this year.:>)) Leslie
Thank you sis,This one is super gorgeous. Want a piece of this one>? It's a Japanese Peony, different from our usual ones and this color is gorgeous as well as the scent.
very beautiful, Vickie !
thank you elfie
I remember this gorgeous flower from last year. Beautifully captured. I bet you would love it from macro lens too but your little Lumix did a very good job here.
yes that's the one. this is from the first batch of blooms, just finishing. More will come yet. THe little Lumix is pretty good for macros, better than the 50D for sure at least with the lenses I have. I'm still aiming for the macro lens
Beautiful flower, beautifuly captured !!
thanks very much Mattie, unfortunately it blooms for such a short time
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text:
Lake County, Illinois has been awarded Federal Funds made available through the Department of Homeland Security (DHS)/Federal Emergency Management Agency under the Emergency Food and Shelter National Board Program.
Lake County, IL has been chosen to receive $321,381 to supplement emergency food and shelter programs in the county.
The selection was made by a National Board that is chaired by the U. S. Department of Homeland Security's Federal Emergency Management Agency and consists of representatives from American Red Cross; Catholic Charities, USA; National Council of the Churches of Christ in the USA; The Jewish Federations of North America, The Salvation Army; and, United Way Worldwide. The Local Board was charged to distribute funds appropriated by Congress to help expand the capacity of food and shelter programs in high-need areas around the country.
A Local Board made up of representatives from United Way of Lake County, The Salvation Army, Jewish Child and Family Services, Community Action Partnership, Catholic Charities of Lake County, Lake County Workforce Development, The Lake County Board and other key organizations, will determine how the funds awarded to Lake County are to be distributed among the emergency food and shelter programs run by local service agencies in the area. The Local Board is responsible for recommending agencies to receive these funds and any additional funds made available under this phase of the program.
Under the terms of the grant from the National Board, local agencies chosen to receive funds must: 1) be private voluntary non-profits or units of government, 2) be eligible to receive Federal funds, 3) have an accounting system, 4) practice nondiscrimination, 5) have the capability to deliver emergency food and/or shelter programs, and 6) if they are a private voluntary organization, have a voluntary board. Qualifying agencies are urged to apply.
Lake County, IL has distributed Emergency Food and Shelter funds previously with A Safe Place, Northern Illinois Food Bank, Lake County Housing Consortium managed by Catholic Charities Lake County Services, Maristella, Most Blessed Trinity, and PADS Lake County, Inc. participating. These agencies were responsible for providing thousands of meals and hundreds of nights of lodging and emergency shelter
Public or private voluntary agencies interested in applying for Emergency Food and Shelter Program funds should attend the Application meeting on Friday, July 29th from 9:30 - 11:00 AM at the United Way of Lake County offices: 330 S. Greenleaf St. Gurnee, IL. Please RSVP for the application meeting to firstname.lastname@example.com. Please visit www.liveunitedlakecounty.org/efsp for more information on the application and meeting. The deadline for applications to be received is Wednesday, August 10, 2016 at 5:00 PM.
Applications received after the deadline will not be accepted. For further information about the EFSP grants call, Deanna Olmem, Lake County EFSP Local Board Chair at 847-775-1018 or email@example.com at United Way of Lake County.
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text: dunnhumbyUSA, the world's leading customer science company, announced today that it has entered into a multi-year contract with GNC Holdings, Inc. (NYSE: GNC), the nation's largest specialty retailer of health and wellness products. After a rigorous selection process, dunnhumbyUSA was chosen to apply its customer centricity solutions across all facets of GNC's marketing, working closely with GNC to more effectively segment and engage with customers of its more than 6,200 retail outlets and online at GNC.com throughout the United States. This will include personalized communications and offers to shoppers through both online and offline channels. GNC has long recognized that getting closer to individual shoppers and creating sustained loyalty is key to building its competitive advantage. The retailer began down a path to customer centricity more than a decade ago with the introduction of the GNC Gold Card, which currently engages several million GNC customers. Earlier this year, GNC launched the Member Price Program to offer even more value to Gold Card members, making benefits previously offered one week per month now available to customers every day. Joseph M. Fortunato, GNC's Chairman of the Board, President, and Chief Executive Officer, said: "Partnering with dunnhumbyUSA represents a significant investment in our direct marketing efforts to be as customized and relevant as possible to every customer on a more personalized level." Fortunato continued: "The ability to completely understand our customers' shopping patterns provides GNC the ability to target products and offers that each customer desires for their individual health and wellness needs, as well as complementary products that will add value to them achieving their individual goals. Our end goal is to maintain customers for life by providing them the best supplement brand and products in the world, developed by GNC's unique Product Development and Scientific Affairs Department." "GNC's customers are not only very dedicated to their personal health and wellness, they are also very enthusiastic about the GNC brand. So for us, delivering relevance is crucial to growing that customer enthusiasm about the GNC brand. We want to keep those customers coming back for more and more, year after year," said Stuart Aitken, CEO of dunnhumbyUSA.


text:
Rough Trade Shops
Magazine - Issue 7
The very autumnal Issue 7 of Rough Trade Magazine contains the following treats for your eyes and...hands?
• An interview with D.D Dumbo conducted in the back seat of his car in his home town of Castlemaine, Australia.
• Fantastic reviews of our top 10 albums of the month by Rough Trade's Delia Sparrow
• Jack Sills of Light in the Attic speaks of his love for 90s lowrider culture in LA
• Writer Craig Taylor reviews cult book The Return of the Soldier by Rebecca West• Elizabeth Skadden is interviewed about her fascinating life by Rough Trade's John Webb
• Ricky Gervais (yes, THE Ricky Gervais) is interviewed about the music that shaped his life by Liv Siddall
• Jof Owen discusses his fear of brains• A funny Skype convo between Jack and James of Ultimate Painting and Sean Yeaton from Parquet Courts
• Brian Boone's "Entries on Rolling Stone's Top 500 Albums of all Time List Whose Titles Sound like Fart Jokes"
• A love letter to Bruce Springsteen by Rough Trade's Gaz Moore
• A convo between cute brothers Michael and Brian of The Lemon Twigs, photographed in a park near the shop by Anna Victoria Best
• Jonathan Richman responds to a question about the importance of French musicians
• A piece about our Label of the Month Trouble in Mind by writer Marc Masters
• An interview with Honnda by Rough Trade West's Tom McDowell
• A horoscope from Sunflower Bean, very much worth a read.
• A comic by Daniel Brereton inspired by the melancholy of Arthur Russell


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
How do I clear some space in upload space?Asked by TechieBloke on January 06, 2015 at 09:49 AM
My account is showing that I have 135mb of upload space?
What is this and what makes up this 135mb?
How do I clear some of it to make room for new stuff?
JotForm Support
Hello TechieBloke,
Upon checking your account, I found that your form "Website Updates Form" has 63 uploads which seems to utilize 135.12 MB of upload storage space.
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text:
I have some videos with "TV Show" as type in iTunes info.
They work fine in iTunes
I copied some over to my iPad and now I cannot find them to play them.
They do not show under videos, only movies.
How do I get them to show or play on my iPad?
9 Replies
Jul 5, 2012 at 2:43 UTC
Brand Representative for Contronex - G Data Distributor
I think they are in the Videos Section still. Under a separate category. The other thing to try is to just change the media type to movie and resync.
Jul 5, 2012 at 3:01 UTC
they are there because i can see them in itunes
the question is how do you see the other sections IE TV shows?
Jul 5, 2012 at 3:06 UTC
Brand Representative for Contronex - G Data Distributor
Well on mine the TV Shows show up on a tab in the video app. If you search for the name of the show, do they show up there?
Jul 5, 2012 at 3:12 UTC
Mine just has a "store" and "edit" button
if i use edit it just lets me delete
Jul 5, 2012 at 3:20 UTC
Brand Representative for Contronex - G Data Distributor
Hmm. Do you know if there are any restrictions setup on that iPad, like that you can't watch TV Shows of a certain rating?
Jul 5, 2012 at 3:26 UTC
shouldnt be
it is mine not company
Jul 5, 2012 at 3:33 UTC
Brand Representative for Contronex - G Data Distributor
Ok I just thought of something. Check in iTunes under Get Info. Make sure that the media type is TV Shows (Which I bet it already is) and then make sure that you have all of the fields filled in info wise, especially the show name
Jul 9, 2012 at 7:37 UTC
That is all set good
The problem is when I go under videos, I only see movies. There is no tabs or anything to switch to TV shows.
Jul 12, 2012 at 6:15 UTC
When you have the iPad3 plugged in, can you browse it in iTunes and see whether it's actually sync'd over?
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Gravity Box
So now it can be told…
I have a new book coming out this May. It is my first short story collection* and will be published by
Walrus Publishing, a small press right here in St. Louis.
Here's the cover, done by the remarkable John Kaufmann, also local to the area.
There are eleven stories, a mix of previously published and new, a mix of science fiction, fantasy, "slipstream," and a mix of short story and novelette. I am, needless to say, very excited about this, and I throw myself on the mercy of anyone reading this to spread the word.
Tentatively, there are plans in the works to have a release party at Left Bank Books in mid-May. I will also be at ConQuest in Kansas City over Memorial Day Weekend where copies will be available.
The first publicity post is up at the Walrus site, here, which is an interview. So rather than ramble on about the book here I urge you to click through and read the interview…and while you're there check out some of Walrus's other titles.
*Hmm. Not strictly true. I should say this is my first BOOK LENGTH short story collection. The estimable Steve Miller and Sharon Lee published a chapbook of three stories some years ago called Other Ways: Three Tales From The Secant. As of this writing, I do not know if these are still in print.
10 thoughts on "Gravity Box"
Congratulations! Can't wait to read it.
Congrats Mark! I'm looking forward to reading.
Absolutely fantastic. Looking forward to reading it. Also, have a new book out in April. It's 40 b & w photos with forty poems.
Congrats, Mark! Please bring copies to your library program in April. I'm looking forward to reading it–and buying copies for friends.
Congrats Mark. Looking forward to it!
Hurrah! It's about time! Can't wait to read it!
Oh, COOL! I have always loved your short-story gemstones best. Sure do miss putting up my feet, holding a vessel of coffee or wine, closing my eyes, and letting the journey begin…. Hey, there's a thought: future audiobooks in your excellent reading voice?
Comments are closed.


text:
Upon indemnifying a policyholder for a loss, an insurer generally has the right to recoup its loss by pursuing recovery from other entities or individuals who may be responsible for the policyholder's damages in the first place. Subrogation rights vary across jurisdictions and can have serious consequences both for the policyholder and the insurer.
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text:
Spend a fun filled day with Salty Tours and give your kids something to talk about! On our kids towable tube ride they get to glide across the lagoon's smooth waters enjoying the safe and adrenaline filled experience. Smiles, pure enjoyment and great memories guaranteed.
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text:
Alexander Nimmo's Inverness Survey and Journal, 1806
Edited by: Noël P. Wilkins
Alexander Nimmo was Rector of Inverness Academy in 1806 when Scotland's Commission for Highland Roads and Bridges employed him to survey the boundary of Inverness-shire with the adjacent counties. During his survey, Nimmo kept a MS journal (c.17K words) of his 'perambulation.' This journal is a valuable and interesting first-hand account of aspects of the shire and the Highlands at a most important time in its history. Additionally, it is a seminal document in Nimmo's personal transformation from schoolmaster to engineer. Nimmo later resigned his Rector's position in 1811 and took up a post as an engineer with the Commission for the Bogs of Ireland, and he went on to complete amazingly comprehensive and unsurpassed surveys, maps, and reports on the Iveragh peninsula of County Kerry and Connemara in County Galway. This book presents a transcription of the Inverness journal along with an introduction and commentaries, and includes a map showing the territory covered by Nimmo during his 'perambulation.' The commentaries place the man and his survey in the context of: his career as a schoolmaster, the historical events of the time in Scotland and Ireland, the map-making of the region during the period, and Nimmo's subsequent engineering career in Ireland and Britain. It is the first time that Nimmo's journal has been published.
208 pages
Publication Date: 6/25/2011
Format: Cloth


text:
iOS Developer - Portsmouth/Part Remote - Greenfield Project
iOS Developer - Portsmouth/Part Remote - Greenfield Project Austin Fraser has a brand new requirement for an experienced iOS developer to join a small software team within the innovation hub of an e-commerce business. This role is a rarity as it is basically a start up within a corporate with clear values when it comes to innovation. In the time the business has been operational they have gone from strength to strength winning multiple awards including Investors in People. This position is best placed for a iOS Developer who is looking to take a step up and utilise technical abilities with design as well. The position is Greenfield so you will see an application through from design to release. The position is based in Portsmouth based office with the opportunity to work remote, We are looking for experience/knowledge with the following; iOS Swift iPhone/iPad applications UI skills My client appreciates the fact that not everyone would have worked with all of these technologies so applications welcome from candidates with different technical background and not all of the above. In return for your expertise you would join an organisation that recognises talent and has won lots of awards for their training and development. Benefits include training courses, flexible working and bonus. For further information and a detailed discussion and an extended job specification please contact Gavin Stephens at Austin Fraser. Desired Skills and Experience iOS Objective C iPhone/iPad This job was originally posted as www.cwjobs.co.uk/job/66901520


text:
Molecular cloning of doublesex genes of four cladocera (water flea) species
Comparative and evolutionary genomics
The gene
We identified homologs of both
The all dsx genes of five cladoceran species examined had similar amino acid structure containing highly conserved DM and oligomerization domains, and exhibited sexually dimorphic expression patterns, suggesting that these genes may have similar functions for environmental sex determination in cladocerans.
Sex determination is a fundamental developmental process, affecting the sexual differentiation of gonads, and leads to sex-specific differences in behavior, physiology and morphology. Sex-determining systems can be divided into two categories: genotypic sex determination (GSD) and environmental sex determination (ESD)
Natural selection of rare mutational variants has been suggested to mediate the transitions between GSD and ESD
Sex determination systems in insects vary considerably in key factors and regulatory mechanisms to develop sex-specific traits. The sex determination mechanism in
The Cladocera (commonly called water fleas) is an ancient clade of branchiopod crustaceans comprising 16 or 18 family lineages
Recently, we and others found that male production occurred independently of environmental cues by treatment with exogenous juvenile hormone (JH) or its analogs
To understand the molecular and evolutionary relationships between GSD and ESD, we previously identified and analyzed three DM-domain genes (DMRT11E, DMRT93B and DMRT99B) from
Results and discussion
Molecular cloning of
To verify whether homologs of
Additional file 1
RT-PCR of oligonucleotides corresponding to highly conserved region of
Click here for file
Figure 1
Nucleotide sequence comparison of
Nucleotide sequence comparison of
Figure 2
Schematic diagrams of the DSX1 structures and its sequence comparison of DM- and oligomerization-domains
Schematic diagrams of the DSX1 structures and its sequence comparison of DM- and oligomerization-domains. (A) Domain structures of DSX1 in
Figure 3
Schematic diagrams of the DSX2 structures and its sequence comparison of DM- and oligomerization-domains
Schematic diagrams of the DSX2 structures and its sequence comparison of DM- and oligomerization-domains. (A) Domain structures of the DSX2 of
Figure 4
Phylogeny of DM-domain containing genes based on amino-acid sequence conservation
Phylogeny of DM-domain containing genes based on amino-acid sequence conservation. The evolutionary history of DM-domain containing genes was inferred by using the Neighbor-Joining method. The percentage of replicate trees in which the associated genes clustered together in the bootstrap test (1,000 replicates) is shown next to the branches (Bootstrap values below 70 percent are not shown). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The analysis involved 55 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 62 positions in the final dataset. Evolutionary analyses were conducted in MEGA5
By comparing the DapmaDsx1 sequence to orthologs from the four studied species (
The DM-domain contains zinc chelating residues, and among the insects studied to date, two highly conserved amino acid residues, threonine and glutamine (Boxed in Figures
Compared to the DM-domains, more amino acid variation is observed in alignments of the oligomerization-domains (Figures
Additional file 2
Estimation of evolutionary divergence between the DSX1 except for DM- and oligomerization-domain and COI sequences. The number of amino acid differences per site between sequences are shown. The analysis involved 5 amino acid sequences. All positions containing gaps and missing data were eliminated. There were total of 225 and 208 positions in the final dataset of DSX1 and COI, respectively. Evolutionary analyses were conducted in MEGA5
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Additional file 3
Estimation of evolutionary divergence between the DSX2 except for DM- and oligomerization-domain and COI sequences. The number of amino acid differences per site between sequences are shown. The analysis involved 5 amino acid sequences. All positions containing gaps and missing data were eliminated. There were total of 210 and 208 positions in the final dataset of DSX2 and COI, respectively. Evolutionary analyses were conducted in MEGA5
Click here for file
Sex specific expression of
We previously reported that both
In this study, we confirmed that expression patterns of
Figure 5
Relative transcriptional expression levels of
Relative transcriptional expression levels of
Annotation of
We previously cloned and described the mRNA transcripts of the
Additional file 4
Gene model annotations on the
Click here for file
Potential transcriptional regulatory elements in the 5' upstream promoter regions of the
To further annotate putative functional and conserved elements of the
We characterized putative known transcription factor binding sites (TFBS) in the
Additional file 5
Nucleotide sequence comparison of
Click here for file
Additional file 6
Nucleotide sequence comparison of
Click here for file
Additional file 7
Nucleotide sequence comparison of
Click here for file
Additional file 8
Click here for file
Additional file 9
Click here for file
Additional file 10
Click here for file
Figure 6
Annotations mapped onto Pro-Coffee alignment of
Annotations mapped onto Pro-Coffee alignment of
We compared the number of unique predicted transcription factors (TFs) shared amongst the
Additional file 11
TF-map alignments unique TFs comparison.
Click here for file
Figure 7
Venn diagram of putative transcriptional factors and sequence logos of
Venn diagram of putative transcriptional factors and sequence logos of
Since we previously reported that
In order to assess whether these
Additional file 12
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In summary, we identified the orthologs of
Isoclonal strains of
Cloning of
The nucleotide sequences of the
Additional file 13
Primer sequences for 5' and 3' RACE in
Click here for file
Phylogenetic analysis of the DM-domain genes
A phylogenetic tree of DM-domain genes including newly cloned
Additional file 14
Accession numbers of the sequences for phylogenetic analysis.
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Quantitative PCR
Two to three weeks old male and female animals of the five cladoceran species were used in quantitative-PCR (q-PCR) assays of gene expression levels. mRNAs were quantified as described previously
The primers were chosen to amplify short PCR products of 150 bp; the primer sequences are listed in Additional file
Additional file 15
Primer sequences for quantitative PCR.
Click here for file
The genomic locations of
Transcription factor map alignments
Additional file 16
Click here for file
Additional file 17
Click here for file
Additional file 18
Click here for file
Additional file 19
Click here for file
Additional file 20
Click here for file
Additional file 21
Click here for file
The 5′ 1.0 Kbp upstream region was extracted from all
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
KT, YK, HM, HW, NT and TI designed the experiments; KT, YK, MS, NS, SM, HM, SO, YO, CH and TM performed the experiments; KT, HM, JKC, TI, SP and CJ analyzed the data; KT, HM, CJ, JKC and TI wrote the paper. All authors have read and approved the final manuscript.
The evolutionary dynamics of sex determination
Establishing sexual dimorphism: conservation amidst diversity?
Sex determining mechanisms: an evolutionary perspective
Evolution of sex chromosomes in Sauropsida
Mode and tempo in environmental sex determination in vertebrates
Sex ratios and conditions required for environmental sex determination in animals
Evolution of environmental sex determination from genotypic sex determination
Models of polygenic sex determination and sex ratio control
The evolution of sex-determining mechanisms: lessons from temperature-sensitive mutations in sex determination genes in
Analyzing the coordinated gene network underlying temperature-dependent sex determination in reptiles
Sex-determining mechanisms in insects
Function and evolution of sex determination mechanisms, genes and pathways in insects
Sex determination in beetles: Production of all male progeny by parental RNAi knockdown of
Molecular phylogeny of the Branchiopoda (Crustacea)–multiple approaches suggest a 'diplostracan' ancestry of the Notostraca
A phylogenetic analysis of the Conchostraca and Cladocera (Crustacea, Branchiopoda, Diplostraca)
Phylogenetic evidence for a single long-lived clade of crustacean cyclic parthenogens and its implications for the evolution of sex
Sexual reproduction in
Effects of endcrine-active chemicals on the development of sex characteristics of
Intersex and male development in
Exploring embryonic germ line development in the water flea,
Diapause induction in
Genotypic characteristics of cyclic parthenogens and their obligately asexual derivatives
Juvenoid hormone methyl farnesoate is a sex determinant in the crustacean
Production of male neonates in four cladoceran species exposed to a juvenile hormone analog, fenoxycarb
Control of sex in Cladocera. III. Localization of the critical period for control of sex
Juvenile hormone agonists affect the occurrence of male
Sequence divergence and expression of a
Sex and the single cell. II. There is a time and place for sex.
Dimerization of doublesex is mediated by a cryptic ubiquitin-associated domain fold: implications for sex-specific gene regulation
Evidence for evolutionary conservation of sex-determining genes
Sex and the singular DM domain: insights into sexual regulation, evolution and plasticity
Genetic and molecular insights into the development and evolution of sexual dimorphism
Molecular cloning and sexually dimorphic expression of DM-domain genes in
Analysis of expressed sequence tags of the water flea
Development of an RNA interference method in the cladoceran crustacean
Environmental sex determination in the branchiopod crustacean
The ecoresponsive genome of
Identification and characterization of the
Evolutionary dynamics of the DM domain gene family in metazoans
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
Structural rRNA characters support monophyly of raptorial limbs and paraphyly of limb specialization in water fleas
Use of ChIP-Seq data for the design of a multiple promoter-alignment method
Transcription factor map alignment of promoter regions
JASPAR, the open access database of transcription factor-binding profiles: new content and tools in the 2008 update
TRANSFAC(R): transcriptional regulation, from patterns to profiles
WebLogo: a sequence logo generator
Morphological changes in
Abortive meiosis in the oogenesis of parthenogenetic
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
Cloning and characterization of the ecdysone receptor and ultraspiracle protein from the water flea
Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies
wFleaBase: the
AnnotationSketch: a genome annotation drawing library
WeederH: an algorithm for finding conserved regulatory motifs and regions in homologous sequences


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Editors' Note: The "Download Now" link directs you to the iTunes App Store, where you must continue the download process. You must have iTunes installed in order to open the link, and you must have an active iTunes account to download the application. This download may not be available in some countries.
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From DigitalSpecks.com: Go back in time to play against the cowboys and check your speed and agility at a shootout contest. Are you the quickest gun that ever lived?
What's new in this version:
1) Advanced Gameplay2) Multiple Enemies3) Smoother Levels4) Fixed Issues
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Tags » Abbott Northwestern Hospital
My Entry into The Leading Physicians of the World
Hey Everyone! Check out my official entry into The Leading Physicians of the World:
Top Cardiologist Raymond W. Scallen, MD, FACC to be Published in The Leading Physicians of the World as New Member of the International Association of HealthCare Professionals
Abbott Northwestern
Name of Hospital: Abbott Northwestern
City, State/Province, Country: Minneapolis, Minnesota, USA
Number of Stars: 4
Comment: I spent some time in both the partial and inpatient programs here and overall was there for almost a month. 571 more words


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Fareportal is one of the fastest growing travel technology companies in the world; our portfolio of travel brands, including flagship product CheapOair, receives over 100 million visitors annually. As a Product Manager, you will be a member of the world-class Fareportal Technology Team which is responsible for creating industry-leading digital products enjoyed by millions of consumers across the globe.
We are seeking a customer-centric, technical product manager for our content management system who possesses at least 3 years of experience building CMS that enhance and improve our user experience. This role's main focus is to define and execute a roadmap based on business vision, market trends, customer feedback and business analytics for our global content management system (GCMS). The right person will possess strong technical acumen and proven experience with the full product life cycle. This is an interesting opportunity for ownership over a critical system that supports 10's of millions of visits a month.
Personal Impact:
Our casual company culture boasts innovation, collaboration and harbors a unique entrepreneurial spirit.
The right people will. . . .
Mission: To be the world's best flight booking and customer experience company with a human touch


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The Most Popular Halloween Costume in Every State
What everyone will be wearing this year, from California to New York.
In case you're still in search of the *perfect* Halloween costume (it's less than two weeks away, you know), here's what everyone else will be wearing.
The folks at Influenster surveyed over 42,000 people across the country, collecting the deets on their costume choice for 2015. According to their report, 84 percent of the U.S. population will be dressing up for Halloween—so don't be one of the lame crowd that doesn't. Of that way-more-fun group, Influenster determined the most popular costume for each state.
The super-original American public has made the minions and Frozen's Elsa this year's top picks. (Seriously? Frozen came out almost two years ago, guys.) Beyond that, Disney princesses and characters still reign supreme in several states. (Surprise, surprise.) Alice in Wonderland took the top spot for California while Snow White swept New York, Pocahontas proved most popular in Georgia and Florida, and Cinderella in several Midwestern states. To name a few.
See the full costume break-down from coast to coast in the infographic below:


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login with
Home remedies for pneumonia
This is not one of those things you should be treating at home without doctors advice. It depends on the type of pneumonia they have as to how it is treated. Bacterial pneumonia will need special antibiotics and possibly aspiration depending on how bad it is. Walking pneumonia may just require rest and lots of liquids.
Either way, seek medical help and follow the advice given by your doctor.
Yeah, that's what I was thinking too. Though I would still be proactive in using essential oils on the chest since it only takes 20 minutes for essential oils to reach every cell in the body.
I agree with you!
Moms also like
About Melissa
Birth: December 31
On Moms.com since: Mar 3, 2014
I am a single mom of two fantastic kiddos that I love to pieces. Currently in school working towards my teaching degree. You can find me most days on www.mommathoughts.com when I am not here chit chatting! :)


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A few weeks ago, my friend and colleague Todd Defren, wrote a post for AOL that seemed to stoke a lot of fires amongst the millennial workforce. Todd dares to tell the truth about how he feels when people quit his agency, SHIFT Communications. What the world needs are more people like Todd who are willing to share how they feel and how they manage people in their employ. We've all become so passive and politically correct, the pink elephants in the room keep multiplying.
That brings me to the past week where I too lost an employee to a new opportunity. Jenna Bromberg, who has been central to our successful and award winning content strategy at H&R Block, left for a great opportunity with Yum Brands – in particular, Pizza Hut. Jenna had a background in the hospitality industry (I stole her from Houlihan's) and wanted to get back to her passion.
Although it hurts in the near term, Jenna was leaving for a great opportunity and following her passion. As her supervisor the past two years, I am proud she's moving on. Not only did she do amazing work for me, but any leader should be happy when their people move to a great job – whether that's internally or externally with someone else. Although I get paid for my functional knowledge and to lead a team, I pride myself on developing others. It's what I love about management.
Back to Todd's post and it's point – there are proper ways to quit and there are bad ways to quit. I was again fortunate here as Jenna quit well. How did she quit well and why should you pay attention? Here's what she did and why it made me a bigger fan:
Clearly, these show the growth and maturity of a true professional. That's why she's on to manage her own team for one of the world's biggest brands. Second, it serves as an example to everyone who may consider that next role. How you leave your job sometimes is as important as the job you did when you were there. That may seem unbalanced but just a you want to make a good first impression, you also want to leave a good lasting impression on your way out.
Thank you for your contribution the past two years Ms. Bromberg. Thanks for your presence here and thanks for leaving us in better shape than when you walked in the door.
God speed you wacky Canadian!
Very classy, Scott.
I've not had the opportunity to work with Jenna, but her reputation is consistent with your resounding endorsement. She's going to continue to do great things, I'm sure.
As a former hiring manager, I agree with everything you wrote about the importance of a smooth exit.


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Hollywood in Vienna
Masterpieces of film music performed by the Vienna Radio Symphony Orchestra under the direction of Keith Lockhart, the Neue Wiener Stimmen (New Vienna Voices) choir and great singers - that's "Hollywood in Vienna" at the Wiener Konzerthaus. The host for the evening was Hollywood film expert Steven Gätjen.
The Sound of Space
In 2016, "Hollywood in Vienna" promised to be an unforgettable concert journey to the fantastic worlds of space and time.
The music to 2001: A Space Odyssey allowed audiences to experience the infinite dimensions of the universe. In Interstellar, between the stars, humanity of the future runned into difficulties finding new inhabitable worlds. The light and dark sides of the Force battled it out in the world-famous Star Wars.
Then Star Trek's starship Enterprise warped into view to explore distant galaxies where no one has gone before. In Contact, signals were received from alien lifeforms.
In Close Encounters of Third Kind, a five-tone phrase enabled communication. Finally, everything revolved around E.T.'s most ardent wish: to phone home.
Tribute to Alexandre Desplat
The second part of the gala concert honored the composer, Oscar and Golden Globe winner Alexandre Desplat. He created atmospheric film music sounds, including for Harry Potter, Twilight, The King's Speech, The Imitation Game and The Grand Budapest Hotel.
Alexandre Desplat said, when he received the reward:
"Thank you Max Steiner, thank you Vienna. It has been an incredible moment to be able to go to the Leopold Museum, the Sezession, the Albertina and Belvedere and see the masterpieces that we knew from books live, like Dürer at the Albertina, and of course being here on this Podium with Keith after so many otters incredible composers and conductors like Anton Bruckner is something hard to believe."
Hollywood in Vienna 2016
13 October 2016, 7.30 pm (pre-premiere)
14 October 2016, 7.30 pm (gala with prize-giving)
Vienna KonzerthausLothringerstraße 20, 1030 Wien
- Your benefit with the Vienna City Card: 10% off
Main entrance
- no steps (Double swinging doors 140 cm wide )
- Ramp 435 cm long
Car parks
Main entrance
- 2 Parking spaces for people with disabilities
Elevator available
- 94 cm wide and 190 cm low , Door 90 cm wide
Further information
- Seeing eye dogs allowed
- Wheelchair accessible restroom available.
All halls accessible via elevator, inner ramp.
2 elevators from ground floor (door width: 90 cm, cabin depth: 190 and 140 cm, cabin width: 94 cm).
Wheelchair seats: 18 in the Grand Hall, 6 in the Mozart Hall, 3 in the Schubert Hall and 4 in the Berio Hall. Seeing-eye dogs permitted (by prior arrangement).
- Main entrance


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Milton Teagle Simmons (born July 12, 1948), known professionally as Richard Simmons, is an American fitness personality who promotes weight-loss programs, most famously through his Sweatin' to the Oldies line of aerobics videos and DVDs and is known for his eccentric, outgoing personality. Simmons began his career opening a gym catering to the overweight, and he became widely known through exposure on television and through the popularity of his consumer products. He became a striking cultural icon, often parodied, and a frequent guest of late night television talk shows such as The Late Show with David Letterman. Today, Simmons continues to promote health and has broadened his activities to include political activism, notably in support of a bill mandating non-competitive physical education in public schools as a part of the "No Child Left Behind" act.
In "Ready, Willing, and Disabled", a cutaway shows Peter Griffin working out in "Richard Simmons' Sweatin' to Books on Tape", a parody of Simmons' "Sweatin' to the Oldies" series of exercise videos. The book on tape is Tuesdays With Morrie by Mitch Albom.
Peter also asks several tough-looking guys at The Drunken Clam if they are Simmons in "Death Is a Bitch". He tries to provoke them into shooting him as a way to prove Death's incapacitation.


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What was basis of the apartheid regime in South Africa?
What was basis of the apartheid regime in South Africa?
Aparthied was particularly oppressive for the blacks.
It was unique to south africa.
The apartheid regime differentiated people on the basis of their skin colour. The native people of South Africa who were black skinned were called blacks. They made up about three-fourth of the population. People of mixed races who were called coloured. The White Europeans were called Whites. The white rulers treated all non-whites as inferior.
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