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- Description
A super lightweight laptop sleeve that protects like its heftier counterparts. Lined with super-soft felt and available in vibrant colors, this sleeve will make a statement in or out of your bag.
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Clinton Township
Man on loose after robbing bank
By Nick Mordowanec
Posted October 11, 2013
UPDATE - Alleged robber caught by FBI - www.candgnews.com/news/fbi-catches-alleged-clinton-township-bank-robber
A suspect was on the loose after robbing the Huntington Bank at 36921 Harper in Clinton Township.
The suspect entered the bank at approximately 11:45 a.m. on Oct. 9. He approached the teller and handed over a note that demanded a large amount of cash. The suspect also produced a silver handgun. The teller handed the suspect an undetermined amount of cash before the suspect fled into the apartment complex east of the bank. It is believed that he then entered an unknown vehicle.
The suspect was described as a black man, estimated to be in his mid-to-late 20s, about 5-foot-7 or 5-foot-8 with a medium-dark complexion. He was wearing a navy blue Detroit Tigers baseball cap with white trim, aviator sunglasses, a navy blue T-shirt, khaki shorts and white shoes.
The Clinton Township Police Department is requesting the public's assistance in identifying the suspect. Contact Det. Bryan Gilbert at (586) 493-7845 if you have any information. Callers can remain anonymous.
About the author
Nick Mordowanec covers Fraser, Clinton Township, Fraser Public Schools, Clintondale Community Schools and Baker College for the Fraser-Clinton Chronicle. Nick, a graduate of Michigan State University, has worked for C & G Newspapers since 2013 and has won awards from the Society of Professional Journalists Detroit Chapter and the Michigan Press Association. He has slight obsessions with "Seinfeld" and Led Zeppelin.
More from C & G Newspapers
Oakland County mosquitoes test positive for West Nile virus
Oakland County
Lahser Road closes for construction
Police search for armed robbery suspects

subset: commoncrawl


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Is your air conditioner problem? Unable to function normally, as usual, it means your air conditioner put out warning signs for improvement. Sometimes these symptoms come at an inopportune time, such as during the summer, was sleeping, even when there are guests who visit your home. Before contacting AC repair services such as aircon service, first you know the signs when your air conditioner is damaged; AC Not Be Turned, this sign may be the worst and certainly, you do not want. If your air conditioner fails when it is turned on, you may be experiencing these symptoms. If the system can not run, it does not mean your air conditioner has broken, however, you should soon be possible to correct your air conditioner from the root of the problem. Perhaps this is because of The amount of damage a lot of your AC system or your electrical problems.
Strange sound or smell; Things are easily known when the air conditioning system is broken. If you suddenly can hear or smell of your air conditioner, there may be a problem from the air conditioner. Sound out or smell is usually an indication of alerting you to a big problem. Not Consistent temperature and air flow; Is your air conditioner a little stuffy? Ventilation, similar to the nose, can sometimes occur due to blockage by debris. This problem can affect the overall temperature of your house because of lack of air flow. If ventilate is in trouble, can pose a threat to your air-conditioning system, as well as your family because they could potentially be infected with dangerous allergies.


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If an item you ordered from TextbookRush does not meet your expectations due to an error on our part, simply fill out a return request and then return it by mail within 30 days of ordering it for a full refund of item cost.
Learn more about our returns policy
Description: We have entered the age of "peep culture": a tell-all, show-all, know-all digital phenomenon that is dramatically altering notions of privacy, individuality, security, and even humanity. Peep culture is reality TV, YouTube, MySpace, Facebook, Twitter, over-the-counter spy gear, blogs, chat rooms, amateur porn, surveillance technology, Dr. Phil , Borat , cell phone photos of your drunk friend making out with her ex-boyfriend, and more. In the age of peep, core values and rights we once took for granted are rapidly being renegotiated, often without our even noticing.With hilarious, exasperated acuity, social critic Hal Niedzviecki dives into peep, starting his own video blog, joining every social network that will have him, monitoring the movements of his toddler, selling his secrets on Craigslist, hiring a private detective to investigate him, spying on his neighbors, trying out for reality TV shows, and stripping for the pleasure of a web audience he isnrsquo;t even sure exists. Part travelogue, part diary, part meditation and social history, The Peep Diaries explores a rapidly emerging digital phenomenon that is radically changing not just the entertainment landscape, but also the firmaments of our culture and society.The Peep Diaries introduces the arrival of the age of peep culture and explores its implications for entertainment, society, sex, politics, and everyday life. Mixing first-rate reporting with sociological observations culled from the latest research, this book captures the shift from pop to peep and the way technology is turning gossip into documentary and Peeping Toms into entertainment journalists. Packed with stranger-than-fiction true-life characters and scenarios, The Peep Diaries reflects the aspirations and confusions of the growing number of people willing to trade the details of their private lives for catharsis, attention, and notoriety.Hal Niedzviecki is the editor of Broken Pencil magazine and has published numerous works of social commentary and fiction, including Hello Irsquo;m Special: How Individuality Became the New Conformity .
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All the information you need in one place! Each Study Brief is a summary of one specific subject; facts, figures, and explanations to help you learn faster.
List price: $17.95Copyright year: 2009Publisher: City Lights BooksPublication date: 6/1/2009Binding: PaperbackPages: 256Size: 5.75" wide x 8.75" long x 1.25" tall Weight: 0.880Language: English
by Hal Niedzviecki and illustrated by Marc Ngui
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No one would notice on a scalloping horse
Submitted by Casey on February 2, 2010 6:00 pmOne Comment
Tastespotting: lighting
Apricot Pillow Cookies – traditional Danish recipe.
Foodgawker: Composition – too tight
Tastespotting: lighting
Wow you should see the bad comments on this site about this one!
Glad they found a type O thanks for posting this one!
.-= pegasuslegend´s last blog ..Almond Crusted Stuffed Haddock with Shrimp in Wine Butter =-.


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Energy efficiency
Optimising energy consumption in your warehouse saveguards the environment and also reduces the operating and maintenance costs of the plant. This not only ensures a clear conscience but also secures your long-term investment.
With expert knowledge and many years of experience, SSI SCHAEFER facilitates the implementation of energy-efficient storage right from the planning of the logistics and the facility. Thanks to our unique and wide range of intralogistics services, we think in systems and complete solutions that assure your success both efficiently and long-term.
A selction of topics on which we provide consulting and that we implement together with you:
Logistics planning
- Site selection
Where is the facility located with regard to your customers, does it have the necessary infrastructure? Which distances need to be covered?
- Site optimisation
Are all the existing spaces and buildings being used efficiently?
- What does the overall energy balance look like?
Building planning
- Use of renewable energies
What are the options regarding photovoltaics, geothermics or solar thermals?
- Lighting
Have energy-saving lights been installed? Is there a concept for security lighting?
- Heating
Are there any reasonable additional extras such as pellet heating, district heating from bio gas plants or black lights?
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Is there a feasible energy-optimised design for the roof and facade that could be realised? Are the gates, doors and locks sufficiently insulated?
Energy efficiency right from the start
When planning and implementing an SSI SCHAEFER warehouse and order picking system, energy efficiency is also a fixed part of the system planning work.
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Quezon City, Philippines [ show map ]
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I'm here to meet guys from 18 to 30 years old for friendship, romance, marriage and more.
hi i'm dawn erica 22 from philippines I am funny,cute and simple. I have a big lovable family. And I'm hoping to meet my Prince here who will love me eternally.
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text: Calvin Klein Men's RTW 2009 Italo Zucchelli's collection stayed truer to the brand's identity than any other of the creative director's previous efforts. IsSixWeeksAfterFashionWeek = false WWD Staff Share This Article Reddit LinkedIn WhatsApp Email Print Talk Galleries Beauty Collection roadblock = false You're missing something! SUBSCRIBE NOW Collections Fashion Season Types Fashion Season Years Fashion Show Cities Fashion Show Topics Fashion Shows regions Share This Article Reddit LinkedIn WhatsApp Email Print Talk load comments blog comments powered by Disqus


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PMP Exam Change 2016 (Part 2): In Part 1, we learned about PMI's Role Delineation Study (RDS), PMP Exam Content Outline, PMBOK Guide, and PMP Handbook, and how these influence the PMP Exam. We have all heard that 8 new tasks have been added, few tasks have been modified, and some deleted from the PMP Exam Content Outline. I'll not go into the full laundry list here. Rather, I'll be focusing on the key theme, and provide a list of the new topics that you need to focus on for the new exam. At the end, I'll also provide some guidance for PMP aspirants to help them align their preparation to the new changes. Let's get rolling.
By Harwinder Singh On 2017-06-30T13:32:41Z - 16 comments
PMI Books24x7 (eReads and Reference) is Dead
For the past few days, PMI's Books24x7 site has been down. The website says "We're Sorry - PMI.org will be back soon." This website used to provide more than 250 project management books, many of which are extremely useful for PMP, CAPM, and PMI-ACP exam prep. The access to this website was free for PMI members and was one of the top benefits of PMI membership. I have several blog posts that link to this site. I waited for a few days and finally sent a message to PMI on Twitter enquiring about the status.Books PMI PMI Membership
By Harwinder Singh On 2017-06-30T15:31:23Z - 0 comments
PMI - Wake Up! PMP Handbook is Out of Date since Jan 12, 2016
It's been more than a month since PMP Exam changed (Jan 12, 2016). But Project Management Institute (PMI) is yet to update the PMP Handbook to reflect the changes. Apart from this, the handbook has other glaring errors, which can mislead or confuse PMP aspirants. Such errors are unpardonable given the popularity of PMP Certification across the globe and the multi-billion dollar industry around it.PMI PMP PMP Handbook
Through this short post, I aim to raise awareness among the PMP aspirants about the out-of-date portions of the PMP Handbook and nudge PMI out of slumber and get its act together.
Through this short post, I aim to raise awareness among the PMP aspirants about the out-of-date portions of the PMP Handbook and nudge PMI out of slumber and get its act together.
By Harwinder Singh On 2017-06-30T13:38:59Z - 3 comments
PMP Exam Change 2016: RDS, Content Outline, PMBOK and Handbook - I'm So Confused! (Part 1)
PMI updated the PMP exam on January 12, 2016 to align with the latest PMP Exam Content Outline, June 2015. PMI announced that 25% of the exam questions were updated as a result of this change. Does it mean that my PMP exam preparation need to change? Does my exam prep material also change by 25%? What are the new topics on the exam? Where do I get the study material for the new topics? Should I wait for a few months before taking the new exam? Has the exam passing score changed? What is this Role Delineation Study (RDS) thing? Is the PMP exam still based on PMBOK Guide or is it now based on the PMP Exam Content Outline (what's the difference between the two, BTW)? If you have any of these questions, this post is for you.PMBOK PMP PMP Exam Change 2016 PMP Exam Content Outline PMP Handbook Role Delineation Study (RDS)


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Nissan Almera N15 Series Service Repair Manual PDF Free Download
Nissan Almera N15 series repair manual pdf, service manual pdf, electrical wiring diagrams pdf, body repair manual pdf, presented Nissan Almera, european market.
The program Nissan Almera N15 series, contains the detailed and full description of repair and diagnostics of all units of the automobile, including detailed electric schemes, the moments of inhalings, process of assembly and disassembly of the engine and other units and units, body sizes for an extract of a body, detailed repair transmission automatic and manual, repair of coupling, steering management, the conditioner, all electric components and blocks of the management, the necessary special tool, and as other information about Nissan Almera N15 series.
This repair manual Nissan Almera contains maintenance and repair procedures for NISSAN Almera, model N15 series. In order to assure your safety and the efficient functioning of the vehicle, this manual should be read thoroughly. It is especially important that the PRECAUTIONS in the GI section be completely understood before starting any repair task. All information in this manual is based on the latest product information at the time of publication. The right is reserved to make changes in specifications and methods at any time without notice. The proper performance of service is essential for both the safety of the technician and the efficient functioning of the vehicle. The service methods in this Service Manual are described in such a manner that the service may be performed safely and accurately. Service varies with the procedures used, the skills of the technician and the tools and parts available. Accordingly, anyone using service procedures, tools or parts which are not specifically recommended by NISSAN must first completely satisfy himself that neither his safety nor the vehicle's safety will be jeopardized by the service method selected.
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This award-winning city hotel has a total of 320 rooms and combines luxurious comfort with modern facilities. It is air-conditioned with 24-hour reception,a hotel safe, a currency exchange facility and lift access, as well as dining, conference and parking facilities, WLAN access and a laundry service. It also has its own nightclub.
This hotel's prime location offers a unique blend of modern yet timeless heritage. The hotel is situated in the heart of the banking and commercial centre of Georgetown, within easy walking distance of shopping districts as well as many of the historical and cultural landmarks in Penang. The tourist centre of Khoo Kongsi, Batu Ferringhi Beach and Kek Lok Si Temple can all be easily reached by car.
All rooms come with en suite bathroom, a hairdryer, a direct dial telephone, a satellite TV, a minibar, tea/coffee making facilities, centrally regulated air-conditioning and a safe.
Live entertainment is available at the Carmen Club, located in the hotel's basement.
Guests can enjoy a sunset buffet dinner and excellent views of the city in the one and only revolving restaurant in Penang, Kopi Tiam.
The following are all accepted as methods of payment: American Express, Diners Club, JCB, MasterCard and VISA.
The hotel Georgetown (Ex.City Bayview Hotel Penang) is located right in the heart of Georgetown, the perfect convenient location for business or leisure.Its 25 min from Penang International Airport to hotel by car.
Room Facilities:
Direct dial telephone
Satellite cable TV
Tea Coffee
Central airconditioned


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12 November 2007,
Price realised CHF 29,800
Patek Philippe. A highly unusual, early and historically interesting 18K gold half hunter case perpetual calendar keyless lever watch with unusual month indication
Signed Patek Philippe & Co., Genève, retailed by A.H. Rodanet, Paris, no. 20'953, manufactured in 1862
Cal. 18''' gilt-finished lever movement, 25 jewels, bimetallic compensation balance, wolf's tooth winding, gold cuvette, the white enamel dial with Roman numerals, three subsidiary dials indicating phases of the moon combined with lunar calendar, day combined with constant seconds, date, window for the month to the lower half, in plain circular case with inlaid blue enamel Roman numerals and five minute divisions to the glazed aperture in the front, case numbered, cuvette signed by maker and retailer and numbered, movement numbered underneath the dial
50 mm. diam.
New York +1 212 636 2000
London +44 (0)20 7839 9060
Hong Kong +852 2760 1766
Shanghai +86 21 6355 1766
Accompanied by Patek Philippe Extract from the Archives confirming production of the present watch with hunter case in 1862 and its subsequent sale on 10 March 1874.
This watch is amongst the earliest perpetual calendar watches made by Patek Philippe: already in use before, the firm's perpetual calendar mechanism was patented in 1889. It is furthermore the only example known to date which such an uncommon dial layout. Whereas the month is normally shown in a separate subsidiary dial, the present watch features a small window to the lower part of the dial, visible through the aperture when the front cover is closed. Furthermore an unusually small indication of the days is incorporated within the constant seconds.
Only another early watch with a similar dial layout but with the addition of a retrograde date to the upper half is known to exist to date. This example, movement no. 25'308, is now on permanent exhibition at the prestigious Patek Philippe Museum in Geneva.
Interestingly, since the production of these two early watches in the 1860s, only two other watches by Patek Philippe have been fitted with a comparable dial layout: reference 871, the double dial secular "true" perpetual calendar watches, one made for Seth G. Atwood and offered in this auction, lot 222.
The writer, columnist and collector on how he caught the watch collecting 'bug', and why almost all the watches he ..
This historic event in New York from 13 July to 23 July features more than 300 rare Patek Philippe watches priced from $5,000..
Few dive watches have made quite as big a splash as this distinctive piece from the golden age of watchmaking, offered in ..
Why Vintage Heuer chronographs made between the 1930s and the 1980s are so highly regarded
Overall sales hit £2.35 billion ($3 billion) in the first half of the year, with auction sales up by 29 per cent on the e..
Interior designer and heritage colour expert Edward Bulmer on the modello by Jean-Baptiste van Loo he acquired 27 yea..


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A DIGE study on the effects of salbutamol on the rat muscle proteome - an exemplar of best practice for data sharing in proteomics
Proteomic techniques allow researchers to perform detailed analyses of cellular states and many studies are published each year, which highlight large numbers of proteins quantified in different samples. However, currently few data sets make it into public databases with sufficient metadata to allow other groups to verify findings, perform data mining or integrate different data sets. The Proteomics Standards Initiative has released a series of "Minimum Information About a Proteomics Experiment" guideline documents (MIAPE modules) and accompanying data exchange formats. This article focuses on proteomic studies based on gel electrophoresis and demonstrates how the corresponding MIAPE modules can be fulfilled and data deposited in public databases, using a new experimental data set as an example.
We have performed a study of the effects of an anabolic agent (salbutamol) at two different time points on the protein complement of rat skeletal muscle cells, quantified by difference gel electrophoresis. In the DIGE study, a total of 31 non-redundant proteins were identified as being potentially modulated at 24 h post treatment and 110 non redundant proteins at 96 h post-treatment. Several categories of function have been highlighted as strongly enriched, providing candidate proteins for further study. We also use the study as an example of best practice for data deposition.
We have deposited all data sets from this study in public databases for further analysis by the community. We also describe more generally how gel-based protein identification data sets can now be deposited in the PRoteomics IDEntifications database (PRIDE), using a new software tool, the PRIDESpotMapper, which we developed to work in conjunction with the PRIDE Converter application. We also demonstrate how the ProteoRed MIAPE generator tool can be used to create and share a complete and compliant set of MIAPE reports for this experiment and others.
A variety of high-throughput experimental techniques are available for studying how the protein complement of a sample (the proteome) changes under different cellular conditions, such as during disease processes. The changes observed in individual proteins, or groups of proteins, as experimental conditions vary allow researchers to begin understanding the underlying molecular mechanisms in the cell. Gel electrophoresis (GE) has been employed to study proteins for over four decades
The Human Proteome Organisation - Proteomics Standards Initiative (HUPO-PSI,
In this article we demonstrate how MIAPE GE (gel electrophoresis) and GI (gel informatics) compliant reports can be created easily in practice, through the MIAPE Generator tool
We have performed a study on the effects of salbutamol (an anabolic agent) on the proteome of rat muscle cells. Salbutamol is a type of beta2 adrenergic agonist, which is known to cause hypertrophy in muscle but the underlying molecular mechanisms are not well understood. The aims of the study are to use proteomic technologies to model changes in the development of skeletal muscle cells
In the supplementary material [Additional file
Additional file 1
Supplementary Methods. The materials and methods from this study
Click here for file
Software development
The PRIDE Converter software
Additional file 2
Example mapping file for the PRIDESpotMapper. The csv file can be opened, for example, in spreadsheet software. The file contains information for mapping Mascot dat files to spot coordinates on gel images, and is used to create the final PRIDE XML file.
Click here for file
Once all the resulting files coming from the search engine (Mascot for this version) are joined in a single PRIDE XML using the PRIDE Converter, the execution of the PRIDESpotMapper is straightforward (Figure
Figure 1
The workflow used to construct a PRIDE XML file containing gel spot data, via the modified PRIDE Converter and the PRIDESpotMapper
The workflow used to construct a PRIDE XML file containing gel spot data, via the modified PRIDE Converter and the PRIDESpotMapper.
The DIGE gels were analyzed as described in the Supplementary Methods [Additional file
At the 24 h time point 17 spots of interest were identified: 4 spots were down-regulated, 13 spots up-regulated from which 31 non-redundant proteins were identified [Additional file
Additional file 3
Proteins with changed abundance at 24 h post-treatment. A summary file containing the set of proteins that had modulated abundance, as detected by DIGE, at 24 h post-treatment with salbutamol.
Click here for file
Additional file 4
Proteins with changed abundance at 96 h post-treatment. A summary file containing the set of proteins that had modulated abundance, as detected by DIGE, at 96 h post-treatment with salbutamol.
Click here for file
We have performed gene ontology enrichment analysis on the two data sets using DAVID (24 h [Additional file
Additional file 5
Clusters of functional annotations for proteins at 24 h post-treatment. The output of clustering functional annotations produced by DAVID, on the protein set at 24 h post-treatment
Click here for file
Additional file 6
Clusters of functional annotations for proteins at 96 h post-treatment. The output of clustering functional annotations produced by DAVID, on the protein set at 96 h post-treatment
Click here for file
In summary, proteins involved in ion binding and transport, nucleosome assembly, cell interactions, protein binding and structural proteins seem to be modulated at 96 h, whereas only structural and energy production proteins are affected after 24 h. An immediate effect of the anabolic agent is to produce a structural effect that needs a great deal of energy. It appears a more complex later effect is observed, involving a number of cellular pathways.
Salbutamol is shown to cause muscle hypertrophy, which suggests it may have a similar mode of action as other beta2-adrenergic agonists. This effect on the muscle cell is rapid, and is clearly seen using high magnification microscopy. As anticipated, many of the differentially expressed proteins identified are cytoskeletal. A significant number are also involved in transcription or translation. Skeletal development pathways are activated both at the early and later time points. Up-regulation of ATP synthesis, glycolysis and phosphorylation also seems to be occurring.
Data deposition and MIAPE report generation
MIAPE guideline documents describe the metadata that should be captured about a given proteomic technique, for instance detailing the minimum information that should be reported about the experimental protocols. The MIAPE Generator tool has been developed to assist in the generation of MIAPE-compliant reports, and is freely accessible from
The benefits of producing the MIAPE reports, in addition or instead of a traditional Materials and Methods, are as follows. The report has a standard structure, requesting key details for each stage of the process, ensuring that the experimenter does not fail to report any information that may be important for reproducing the protocols in another lab. As one example, the MIAPE GE document requests that the gel recipe are provided (section 3.2.2) if the gel was not purchased pre-cast. Similarly, the MIAPE GI document requests details should be provided on software parameters and algorithms used with different software packages, which can affect the results obtained. The MIAPE GE/GI specifications also request that raw data should be provided and linked to the report, in this case, the original gel images. This could potentially be hugely valuable if researchers are interested in performing more detailed examination of results, for example to test if a specific protein is differentially regulated, using different statistical assumptions than the researchers who generated the data.
Deposition of protein identification data in PRIDE
The PRIDE database has become one of the leading public repositories for proteomics results. However, to date, few gel studies have been deposited in PRIDE due to the lack of appropriate tools. We have deposited two files - one for the 24 h and one for 96 h time point, each containing the protein identifications for each gel spot. Each protein identification has a link to the gel image in the MIAPE database, along with X/Y coordinates and quantification information, in terms of the ratio detected by DIGE across treated versus control samples. As far as we are aware, this is the first deposition of a complete quantitative DIGE data set in PRIDE. The PRIDE records can be accessed at
Discussion and Conclusions
Each year there are many hundreds of proteomics studies published in the literature, in which gel electrophoresis is used to separate, identify and perform relative quantification of the proteins present in complex samples. However, few of these data sets have ever made it into the public domain, beyond lists of protein spots provided in tables within articles or as supplementary material in spreadsheets. The Proteomics Standards Initiative has released several tools and guideline documents designed to improve the public accessibility of proteomics data, including minimum reporting guidelines (MIAPE documents) and XML formats. The EBI has also developed the PRIDE database to allow proteomics scientists to publish protein identification data sets to the wider community.
While it is possible to include protein identification data in the database behind the MIAPE generator tool, this is not the standard public repository for this kind of data. Instead, proteomics scientists tend to search the PRIDE database (or GPM, PeptideAtlas, Peptidome, Tranche) for identification data. As such, it is important for gel-based proteomics studies to be deposited in one of these primary data repositories. To date, almost no data sets derived from gel-based experiments have been deposited in any of these databases. We have created the PRIDESpotMapper to work alongside the PRIDE Converter and thus, for the first time, provide a simple route for uploading valid PRIDE XML, containing gel spot information and quantitative values. The PRIDE developers may include gel support in future versions of the PRIDE Converter directly. We will work with the PRIDE team to incorporate the same mechanism presented here for representing gel spot data, to ensure that researchers wishing to share gel-based proteomic data can use the PRIDESpotMapper now, and migrate to a new version of the PRIDE Converter, as and when appropriate.
This article should serve as an exemplar for how researchers can upload gel-based data to PRIDE and use the MIAPE Generator tool for creating MIAPE compliant reports. There is on-going discussion with journal editors as to the requirement for proteomics articles to be MIAPE compliant - and these reports may in due course supplement, or in some cases replace, traditional materials and methods sections of proteomics articles. We encourage further discussion of these issues, for example through the PSI's open mailing lists or attendance at the annual PSI meeting.
For the study described, we have created MIAPE reports describing the gel electrophoresis and the gel image informatics performed, and these have been deposited in the associated database. The database also contains the source gel images, allowing other groups to re-analyse these data, using the same or different software pipelines.
The initial results of the study show that several key pathways are modulated by treatment with salbutamol, with significantly more changes occurring at 96 h post-treatment. This indicates that there is a lag between the treatment and the downstream activation of cellular pathways. There are some limitations of the DIGE results, not least that true quantitative ratios cannot be linked to individual protein identities, since the sensitivity of tandem MS revealed that many spots on the gels contained more than one protein. However, the ontology enrichment analysis shows that many of the protein groups highlighted are likely to be direct or indirect targets for salbutamol, since the enriched functional categories fit our expected hypotheses of the effects of an adrenergic agonist. We are making these data sets freely available as we anticipate that they will be useful to other researchers working in this area for building hypotheses about the mechanism of action of salbutamol on the proteome of muscle tissue.
Availability and requirements
The software described in this article is accessible from
The adapted PRIDE Converter described here is released as a snapshot build (2.4.2), which will be updated periodically when there are major new releases of the main PRIDE Converter software but not for minor updates. The PRIDE Converter is freely available and open-source, released under Apache License 2.0. PRIDE Converter requires Java 1.5 (or above). The current version has been tested on Windows XP, Windows Vista, Linux and Mac OS X.
The PRIDESpotMapper is freely available as a Java jar file for local install or can be run using Java web start. The application has tested using Java Runtime Environment (JRE) 1.6 with the following operating systems: Windows 7, Windows XP, Windows Vista, Linux Red Hat, Linux Ubuntu.
URLs to link direct to these records in the MIAPE Generator database.
24 h timepoint:
96 h timepoint:
PRIDE access for data sets
Accessions 16472 and 16473
Mascot dat files located on Tranche:
24 h time point:
96 h time point:
Passphrase: ratproteome
List of abbreviations
DIGE: difference in-gel electrophoresis; GE: Gel electrophoresis; GI: gel informatics; GO: Gene ontology; HUPO: Human Proteome Organisation; LC-MS: Liquid Chromatography-Mass Spectrometry; MIAPE: Minimum Information About a Proteomics Experiment; PRIDE: PRoteomics IDEntifications database; PSI: Proteomics Standards Initiative; XML: Extensible Markup Language
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
JK performed the DIGE study, supervised by JMW. JAMA led the software development work described, assisted by SMB and supervised by JPA. ARJ led the writing of the manuscript and contributed to software development and to analysis of the DIGE results. All authors have contributed text to the manuscript.
Acknowledgements and Funding
We would like to thank Adrian Thompson at Pfizer for providing rat cells. JK is funded on a BBSRC CASE studentship, in collaboration with Pfizer, whose support we gratefully acknowledge. Work on data standards in ARJ's group is funded by the BBSRC [BB/G010781/1].
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
High resolution two-dimensional electrophoresis of proteins
Difference gel electrophoresis: a single gel method for detecting changes in protein extracts
The HUPO Proteomics Standards Initiative
The minimum information about a proteomics experiment (MIAPE)
Guidelines for reporting the use of gel electrophoresis in proteomics
Guidelines for reporting the use of gel image informatics in proteomics
Guidelines for reporting the use of mass spectrometry in proteomics
Guidelines for reporting the use of mass spectrometry informatics in proteomics
Guidelines for reporting the use of column chromatography in proteomics
Guidelines for reporting the use of capillary electrophoresis in proteomics
The minimum information required for reporting a molecular interaction experiment (MIMIx)
The Gel Electrophoresis Markup Language (GelML) from the Proteomics Standards Initiative
PRIDE: a public repository of protein and peptide identifications for the proteomics community
The PeptideAtlas project
NCBI Peptidome: a new public repository for mass spectrometry peptide identifications
Open Source System for Analyzing, Validating, and Storing Protein Identification Data
SWISS-2DPAGE, ten years later
Semi-automatic tool to describe, store and compare proteomics experiments based on MIAPE compliant reports
PRIDE Converter: making proteomics data-sharing easy
Gene Ontology: tool for the unification of biology
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
PRIDEViewer: A novel user-friendly interface to visualize PRIDE XML files


text:
(Osseo wi, United states)
Dear heavanly father, please help me and bring my family back together. My parents have been fighting a lot lately and it's. been stressing me out. My sisters are always gone and I'm the only child here listening to it. I rely on you God and I trust you to make everything better. Thank you for guiding me through evedyday and loving me. Have your angles surround us while we sleep. In your name, Jesus Christ, Amen.
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text:
Dhaanya Kootu or Cereals and pulses mixture, Health mix - buy online
Dhaanya Kootu is a kind of unique combination of cereals and pulses. This mixture of Cereals and pulses would help in the effective build up of mental power and physical health. It is considered to be an ideal food for all ages, especially for the infants and diabetic patients. Dhaanya Kootu or Cereals and pulses mixture would contain Normal rice (നാടൻ അരി), Tapioca pearls (ചൗവ്വരി), Maize (ചോളം), Ragi, Millet, Suji wheat (സൂചിഗോതമ്പ്), Green Gram (ചെറുപയർ), Bengal gram (പൊരിക്കടല) and Poppy seeds (കശകശ). It also contains spices and nuts varieties like Cinnamon (കറുവാപ്പട്ട), Cardamom (ഏലക്ക), Pista, Cashew nuts, Ground Nuts (നിലക്കടല) and Badam or Almond. The sweetening agent used is Rock candy (കൽക്കണ്ടം). Diabetic patients can include mixture in their daily diet as it would help them to control the blood sugar levels.
How to use this mixture?
- Dry fry the cereals, pulses, spices and nuts varieties. Grind the mixture to a fine flour along with the Rock candy (കൽക്കണ്ടം).
- Sieve it through a fine sieve and transfer to an airtight container
- Take a glass of milk and add 1 tablespoon of the powdered mixture. Boil the milk and add some sugar for taste. Have it as a nutritious drink


text: Boy group VIXX has recently made their first comeback of 2015 with the release of Boys' Record, which features the title track "Love Equation". The boys proved their popularity by sweeping this week's music programs, taking home a total of six trophies for their latest single. For "Love Equation" promotions, Ken has been seen wearing a top from Korean brand 87mm. The white S.L.A Sweatshirt ($n/a) has been seen in the official MV and on stage at Inkigayo. It features black striped accents and the phrase "Seoul Like Abnormal" is embroidered on the front.


text:
Related quotes
"I like being the underdog and I like to hear the crowd boo, ... By the end of the fight they'll be cheering for me saying, 'That was a great fight.'"
"I didn't hear the boos, ... They buy tickets, and they can certainly do anything they want. I would probably boo a couple [of the plays]."
"I hit it perfect and it just kept coming back, ... The crowd cheered, and then booed, and it just hung there."


text:
Why do you like or dislike this movie?
Uploaded: 2015-11-06 Rating: % / Votes
Description: We have a sexy hottie babe today wanted a quick cash for the gun that she have so I offer her extra cash if she will pose on it naked. A horny hottie babe wanted a quick cash so I give her a sweet deal to suck and fuck


text:
VNC probe insight
We are happy to announce the DRG VNC probe insight report based on DRG network data. We believe this is the first VNC-based report of it's kind being offered to the community. You can find this new community resource on the Insight & Analysis page. A special thank you to Sangkyun Noh, a DRG contributor, for developing the DRG Distro module that helped make this report possible.
posted at 2:10 pm | permanent link
The debut of DRG Weekend Reads
Today we debut a new service entitled DRG Weekend Reads, a weekly short list of good information security reads to enjoy your weekend with. Each Friday we'll bring you the best of the week, many of which come from the far corners of the net that may have fallen under the popular radar, but above the interest threshold.
posted at 12:00 am | permanent link
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text:
Risk appetite dominates Aussie
Caution is liable to be a key feature over the next 48 hours with unease over the G20 meetings and renewed fears over the US auto sector. In this environment, the Australian dollar will remain vulnerable to a further correction weaker, especially if equity markets are subjected to heavy selling pressure. Underlying sentiment should still be firmer than seen at the beginning of March and capital outflows from Japan will also provide Australian currency support. Overall, there is the risk that the correction weaker will continue slightly further with solid support realistic on dips to the 0.6680 region against the US dollar.
The Australian dollar was unable to make a fresh challenge on resistance above the 0.70 level on Friday and weakened to the 0.69 region in US trading as the US currency gained ground and Wall Street came under pressure. The theme towards weaker risk appetite continued on Monday as Asian stock markets fell significantly and this pushed the Australian currency to lows just below the 0.68 level against the US currency. There will be further caution ahead of the Reserve Bank interest rate meeting this month, although international trends will tend to dominate for now.
International policy responses and co-operation will continue to be watched very closely ahead of the G20 meeting on Thursday. There will be further unease that no significant policy developments will be agreed while there will also be fears of major tensions as any serious disagreements would cause much more serious damage to market confidence. In this environment, risk appetite is liable to weaken slightly further which would tend to benefit the dollar and yen and undermine currencies such as the Australian currency.


text:
Hello, from Sauce. I have a question about the Call of Duty: Black Ops Rezurrection DLC. Last Night, I purchased it, wrote down the codes on the PSN store, and downloaded only the Rezurrection map pack (which is 745 mb, and took 6 HOURS TO DOWNLOAD). This morning, went I went to play it, I was welcomed with the sight that I had only downloaded the map Moon. This afternoon, I wrote the codes down again and is currently downloading the Call of Duty: World at War Map Pack 4 (It will take 12 hours, ending at about 12:00 AM Pacific Standard Time) for BO. Is this why I didn't get the old maps the first time? Thanks. AwesomeSauce360 talk
EDIT: On October 12, 2011, at 6:41 PM PST, I decided to give it one last try. IT WILL ONLY TAKE 3 AND A HALF HOURS!!!! OMGOMGOMGOMGOMGOMGOMG I'M SO HAPPY!


text:
Full Metal Jacket - Animal Mother MP3
One of my favorite scenes in the whole movie. For people who are wondering what the song is playing in the background, its Wooly Bully by Sam the Sham and ...
Animal Mother's Charge MP3
Full Metal Jacket.
Full Metal Jacket - Animal Mother - HD MP3
Watch In High Quality: http://www.youtube.com/watch?v=vDrQ9amRgrc&hd=1 I was a bit bored so I made a tribute about my favorite character in Full Metal ...
Animal mother -Poontang- MP3
The guru gives away the true meaning of life. Listen and learn.
Animal Mother Tribute MP3
I do not own rights to Stanley Kubrick's "Full Metal Jacket" or Metallica's "Holier Than Thou". This is a Tribute vid. for the best movie character ever to live, ...
The 10 Most Amazing Moms of the Animal Kingdom MP3
The animal kingdom at large doesn't celebrate Mother's Day, but there are several moms in it that definitely deserve a huge bouquet of flowers and breakfast in ...
Loadout - M60E4 Animal Mother Style! (Battlefield 3 Gameplay/Commentary) MP3
Loadout - M416 Special Forces http://youtu.be/tP6trgtBnjY Hey guys. It's time for episode 77 of Loadout. LevelCap's Computer Hardware: ...
Animal Mother MP3
Scene from Full Metal Jacket.
Animal Mother MP3
Provided to YouTube by Believe SAS Animal Mother · Today Is The Day Animal Mother ℗ 2014 Southern Lord Recordings ℗ Southern Lord Recordings ...
Full Metal Jacket - Animal Mother & Joker (Italian) MP3
you talk the talk, do you walk the walk?
Metal Gear Solid V: The Phantom Pain Stealth Walkthrough - Mother Base - Animal Platform MP3
This Walkthrough for Metal Gear Solid V The Phantom Pain will show you how to achieve S-RANK on every mission. I will also show you where you can find ...
The Cat & The Ducklings (Animal Odd Couples) MP3
Meet Della and her 'yellow kittens' :)
Animal Mother - Pig Dna Live At BoilerRoom 12/20/2014 MP3
Live Performing Of Animal Mother - Pig Dna at BoilerRoom (BostonMa) 12/20/2014 Enjoy Not Music!!!!
Most Precious Blood - Animal Mother MP3
Artist: Most Precious Blood Album : Do Not Resuscitate Track : #11 - Animal Mother Released: 2011 Style: Hardcore Lyrics : Breathe with hell in your heart and ...
Today Is The Day - Animal Mother MP3
Today Is The Day coming to The Brighton Bar September 26th, all info & tickets here: www.facebook.com/events/1468136056814907.
Animal Mother Love with her Baby MP3
Mother's Love Photos -- National Geographic animals.nationalgeographic.com/animals National Geographic Society Animal mothers bond with their babies in ...
Animal Mother - Live @ Hashtag Bar - "Break My Heart"/ "Dedicated (Sausage & Peppers)" MP3
Performance from the Rising Stars Concert Series held at Hashtag Bar in Staten Island, New York.
Full Metal Jacket - Animal Mother - Charge! MP3
The greatest charge scene in war movie history!
Animal Mother - Better You Than Me MP3
Animal Rhymes | Nursery Rhymes from Mother Goose Club! MP3
Animal Rhymes from Mother Goose Club! Sing along with your favorite Mother Goose Club characters to these classic animal rhymes! Watch "Rhyme With Us!" ...
Happy Mother's Day! Cute Animal Video [Animal Compilation] MP3
A short compilation of loving animal mothers to celebrate motherhood for everyone! Music by Edoardo Bennato.
MGSV:TPP - Animal Conservation Platform MP3
A little tour of the off mother base base where captured animals are kept.
ANTIKYTHERA "Animal Mother" MP3
ANTIKYTHERA performing "Animal Mother" live from their upcoming second full length release Pantheon.
TODAY IS THE DAY "Heathen" Official Video [Southern Lord Recordings] MP3
"Heathen" is culled from TODAY IS THE DAY's tenth studio full-length, Animal Mother, out now on Southern Lord Recordings. A Novel Concept TV Production ...
Animal Mother & Private Joker atışması MP3
Bu sahnemizde animal mother private jokere hayatının ayarını vermektedir.
Animal Mother - Stones Throe MP3
A project for our Music Video production class at Pacific Audio Visual Institute. Animal Mother is: Josh Wyton - Drums Dave Hass - Guitar Sound and Video ...
My Review Of Today Is The Day "Animal Mother" MP3
CLICK FOR LINKS: Stream it here officially/buy it digitally/physically: http://todayisthedaysl.bandcamp.com/releases "Masada" music video: ...
TODAY IS THE DAY "Animal Mother" Live 11/14/2014 The Foundry, Cleveland, OH MP3
Today is the Day performing "Animal Mother" Live at the Foundry in Cleveland, OH on November 14, 2014 Today is the Day are: Steve Austin - Vocals & Guitar ...


text:
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text:
Manic Street Preachers
Larissa Wodtke et al's study of the Manics' album The Holy Bible captures the band's intellectual appeal
Shows such as Tiswas and Going Live! were bastions of British entertainment, but now they've been replaced by wall-to-wall TV chefs. We celebrate its golden era and ask where it went
Fans in Israel were unsatisfied with the Cheap Thrills singer's show due to a lack of between-song chat – but banter only works in the right hands
Every international tournament brings fleeting hope, crushing disappointment and a raft of singles. Hear's your brief guide to the latter
Brief letters: Hospital targets | McDonald's | Manic Street Preachers | Brief Encounter | Margaret Thatcher | Richmal Crompton
The track, Together Stronger (C'mon Wales), will celebrate the Wales football team competing in its first major championships for 50 years
George Bingham, Lord Lucan's son, wants his long-gone father to be officially declared dead. But those who disappear have a tenacious hold on the collective imagination
Last week we brought you our 10 best namechecks in songs. Here we present your thoughts on recordings that should have made the list
From Manic Street Preachers to the Chemical Brothers, some of the UK's best acts have passed through the doors of Heavenly records. As it hits 25, Saint Etienne, Beth Orton and head honcho Jeff Barrett explain how the label pulled it off
Meanwhile Paul Simon scores his first UK No 1 album in 25 years with The Ultimate Collection and the Manic Street Preachers' Holy Bible reissue tops the vinyl charts
When someone disappears, it is often their parents who are thrust into the spotlight. But what of the brothers and sisters left behind? Here, five talk frankly about those they have loved – and lost
'At the end of 2002, the Manics invited us to play with them. My teenage dreams had somehow come true'
No glitz, no glamour, just a measured, unpretentious documentary for the faithful, writes Peter Bradshaw
The Manics go back 20 years to revive the deathly beauty of The Holy Bible and the memory of their lost fourth member, writes Dorian Lynskey
With the Manics taking their landmark album The Holy Bible on tour, Emily Mackay turns away from the long form and celebrates 10 memorable moments
The band's military re-enactment of their caustic 1994 masterpiece gives it a crackling new energy, writes Graeme Virtue
Christmas has come early for Manic fans – lifted from their Holy Bible reissue, here's a previously unreleased version of their second single from the record
The Manics frontman tells Phil Mongredien about the power of music writing, the depth of Badfinger's back catalogue – and how Guns N' Roses shaped his first experience of London
In revealing interview to mark 20th anniversary of The Holy Bible, the last album Edwards worked on, the band say it was difficult to admit how desperate the guitarist was, writes Vanessa Thorpe
Music blog Later...with Jools Holland: Jessie Ware, Manic Street Preachers, Ben Howard - as it happenedThis week's Later... features Jessie Ware and the Manic Street Preachers (plus some other people) having a singsong in a studio
James Dean Bradfield, Nicky Wire and Sean Moore will play a series of shows in December, celebrating 20 years since their third album was released
From the Manics going krautrock to some spectacularly glossy pop, here are five new albums for you to consider this week
Lana Del Rey, Jack White, the Pixies and Robert Plant were the highlights on day two – and of course the main event: Metallica
Twenty years after their notorious festival debut, a set brimming with big hits confirmed the Manics' status as crowd-pleasers, writes Gwilym Mumford
'The beat is slow, like a rusty steam train pushing through a river of hot tar'
John Harris: The Manic Street Preachers are going to Glastonbury with a new album, Futurology, that's full of European influences, from Russian avant-garde and Krautrock to early Simple Minds
Inspired by cultural landmarks and krautrock records they absorbed during their recent tour, watch the band's video for their disco-punk marching anthem
It's a bumper Singles Club, with tracks from Manic Street Preachers, Kelela, Raul Midón and Years and Years
From Russell Brand's revolution rallying cry to rocking with rugby players, the musical stars of 2013 pick the highlights of their year


text:
Welcome to this week's Kickstart, presented by One Industries. The pace of work has been non-stop at the TW offices lately, as we recently closed our December issue and now face January's deadlines, which will include the Racer and FMXer of the Year. Who do you think will land the covers?
Last Wednesday, we premiered the latest in the Kickstart DVD series, Kickstart 4: The Final Chapter, at Marquee 15 in Corona, CA. If you were unable to make it out or would like proof that people wear pick-up kits, see our recap at http://motocross.transworld.net/1000137806/features/kickstart-4-the-final-chapter-premiere-party/
Before heading to our movie, we spotted Travis Baker on a Valli Yamaha YZ450F. Valli and Star have recently ended their partnership and Baker is the first to commit to the Colorado race team. Check the Randy Robert's video for proof http://motocross.transworld.net/1000137926/news/riding-with-travis-baker/
Finally, Swap tossed his video camera aside for a Canon EOS Mark III and caught Jeremy McGrath in action while at the DC gear launch. You know you want to see this http://motocross.transworld.net/1000137869/features/jeremy-mcgrath-photo-gallery/
There is plenty to see and do in this week's Kickstart, including more about the DC gear, A Day in the Dirt, numerous interviews, a rundown of the 2013 GP schedule, and more. Get to gettin'…


text:
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text:
- 49 years old
- Date of birth: Dec 5, 1962
- Date of passing: Aug 29, 2012
|Let the memory of OGBONNA be with us forever
"Don Dusty,
Continue to sleep in peace, Egbon. I miss you like crazy."
"Happy Posthumous Birthday, Dusty. Continue to rest in peace."
"Don Dusty,
i cant even believe that it is 4 years already since you left us behind. continue to rest knowing that Toyin is doing a wonderful job with the boys.
i love you brother and will always miss you."
"Sleep well dusty. You were a jolly good fella but left too soon."
"It's 3 years now, Dusty. Sleep on, Brother."
"Happy Posthumous Birthday, Dusty <3"
"Dusty, you would have clocked 51 today! But you must be in a better place now. Sleep on, Brother."
"Dusty, you would have clocked 51 today! But you must be in a better place now. Sleep on, Brother."
"Continue to rest in Peace"
"Dusty darl, its one year now that you left us. You were my true First Love and the one that lived in me. Although I have had four encounters with you since your passings, all those meetings have different messages, but overall you seem happy. Your song"First Cut is the deepest", you sang for me a month to your untimely death, brought me to tears. Rest on MFL, Dusty. Thanks Agwu London. Kim"
"Dusty, cant believe its a year already. We miss you. Continue to rest in the bosom of our Lord."
"I missed Amadi sooooo much, to the extent I never had the courage to visit his country home when I traveled for the xmas. 'How can I face his grave'!, I thought within myself. May Amadi – the epitome of entertainment reporting in Nigeria – continue to rest in peace."
"I celebrate the fact that you were born and knowing you, even though the cold hands of death snatched you away from us too quickly. Happy Posthumous Birthday, Dusty. Continue to rest in peace.
Forever remembered x"
"Egbon, you would have been 50 today. But God choose to call you home earlier than we would have liked. HE knows better. Continue smiling whereever you are. Happy birthday."
"you were the lord of entertainment reporting in nigeria, your memory lingers forever like the comet, bye bros"
"By Fred Iwenjora
For my friend and bros, Ogbonnaya Dusty Amadi, the Lord of entertainment reporting in Nigeria
The news of the sudden death of my friend, colleague and brother, Ogbonnaya Amadi whom I called the Lord of entertainment reporting in Nigeria made me scream . My host in Mannheim, Germany where I am currently doing a couple of things including being a visiting reporter for African Cour"
"He was dedicated to bringing out the best in young people. Amazing, lovable character x"
"Dusty, your candle blew out but your legend lives on."
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10"w x 10"d x 24"h
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Sitter in Wimberley
I am a professional nanny who has provided care for children most of my adult life. I have a profile at care..... where you can see I have had a clean background check. I am in the process of getting my first aid/CPR re-certification and have a squeaky clean driving record. My university education includes 3 semesters of elementary education and some early childhood education. I have excellent references and absolutely love taking care of children. I am looking for full time (live out) work and know I would be an excellent addition to your family. I am comfortable with pets and always leave your home a little cleaner than it was when you left. I would love to talk with you! Kind Regards, Michelle Moss
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Wichtiges Update Version: KB2726961
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Bitte beachten Sie, für dieses Update existiert keine deutsche Beschreibung.
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Xcom Enemy Unknown Download Free pc Game using single direct link for windows. No waiting , No trials, No survey. Only full version pc game.
XCOM: Enemy Unknown will place you in control of a secret paramilitary organization called XCOM. As the XCOM commander, you will defend against a terrifying global alien invasion by managing resources, advancing technologies, and overseeing combat strategies and individual unit tactics.
The original XCOM is widely regarded as one of the best games ever made and has now been re-imagined by the strategy experts at Firaxis Games. XCOM: Enemy Unknown will expand on that legacy with an entirely new invasion story, enemies and technologies to fight aliens and defend Earth.
You will control the fate of the human race through researching alien technologies, creating and managing a fully operational base, planning combat missions and controlling soldier movement in battle.
Xcom Enemy Unknown Features
- Strategy Evolved: XCOM: Enemy Unknown couples tactical turn-based gameplay with incredible action sequences and on-the-ground combat.
- Strategic Base: Recruit, customize and grow unique soldiers and manage your personnel. Detect and intercept the alien threat as you build and expand your XCOM headquarters.
- Tactical Combat: Direct soldier squads in turn-based ground battles and deploy air units such as the Interceptor and Skyranger.
- Worldwide Threat: Combat spans the globe as the XCOM team engages in over 70 unique missions, interacting and negotiating with governments around the world.
Xcom Enemy Unknown System Requirements
OS:Windows Vista
Processor:2 GHz Dual Core
Memory:2 GB RAM
Graphics:NVIDIA GeForce 8600 GT / ATI Radeon HD 2600 XT or greater
Hard Drive:20 GB HD space
Sound:DirectX Compatible
Other Requirements:Broadband Internet connection
Additional:Initial installation requires one-time internet connection for Steam authentication; software installations required (included with the game) include Steam Client, Visual C++ 2008 Redistributable, DirectX and Microsoft .NET 4.
OS:Windows 7
Processor:2 GHz Dual Core (Intel Core 2 Duo 2.4 GHz or Athlon X2 2.7 GHz)
Memory:4 GB RAM
Graphics:NVIDIA GeForce 9000 series / ATI Radeon HD 3000 series or greater
Hard Drive:20 GB HD space
Sound:DirectX Compatible
Other Requirements:Broadband Internet connection
Additional:Initial installation requires one-time internet connection for Steam authentication; software installations required (included with the game) include Steam Client, Visual C++ 2008 Redistributable, DirectX and Microsoft .NET 4.
Xcom Enemy Unknown pc game Review
I could tell you about the many times I played through this game's campaign. About Enemy Within, and the chances it brought. About the mod Long War and how it turns this great game into an epic marathon. But you don't want to hear that. You want to know if this particular game is worth your money and your time.
Xcom Enemy Unknown is about adaptation. About turning the weapons and technologies of the invaders against them, about choosing where to pool your limited resources, or where to send your troopers to fight, knowing some may not return.
And boy, does it hurt when your beloved sniper, the one you've been watching over from the start, that guy that could always be counted to nail a crit at extreme distances. He has just died because you send your fire team ahead and he was left behind to offer support… until a flanking alien killed him in cold blood. That's Xcom Enemy Unknown. Those memorable moments that happen amidst the familiar landscapes. You'll also offer silent prayer to that unfortunate civilian that had to die so that you could hit that alien cluster around him with a rocket.
In terms of crunch, the tactical aspect is very solid. A large part of the battlefield can be brought down, and so can your cover, making each battle less about hunkering down and dealing and taken fire and more about maneuvering. A flanking, agile soldier can become just as deadly as the one hunkered down behind a wall with the giant machine gun.
A very enjoyable game, with engaging mechanics and a smart AI that makes for very emotional, unscripted moments.
Another Review
The remake of one of the golden oldies of the strategy genre. Naturally this made it quite difficult to satisfy the fans of the original as well as to attract new players in equal parts.
What Firaxis did well, was the graphical style and presentation, XCOM offers the style of the first UFO games, while bringing it in the new era. Nothing extraordinaire for sure, but fitting graphics with the one or other really nice possibility to take a screenshot or enjoy the headshot with which your sniper eliminates this pesky alien.
Regarding the gameplay they tried to keep the balance between streamlining and complexity.
The maps are handmade, offering some nice details and mostly destroyable environment, but since the random element is gone, they keep on repeating themselves after some playtime.
The same can be said for the progression of the game itself.. your soldiers are basically divided into classes, which have fixed perk trees to choose from one level up. Without the added options of "Second wave" and the additional possibilities from "Enemy within" to enhance your soldiers with mech or gene modifications, this brings up a similar problem to the maps, the soldiers are very similar to each other. As mentioned the add-on options change this in a positive way, beside the modifications you can for example enable that each soldier has different starting stats. Still this does not reach the complexity of the original X-Com soldiers, but helps the diversity significantly.
As for the base management and battles themselves.. once again it is the mix of old and new. The Action points of the old soldiers are gone, now each soldier has two actions per turn (with the right perks, it can be way more though) one for moving, the other for shooting. Again perks as well as equipment can change the possibilities of a soldier's actions.
This system together with an intelligent cover system works quite well and makes for interesting battles.
The base has fixed fields, which you have to fill during the game. Also with the Xcom Enemy Unknown the possibility of the enemy invading your base returns, though as a scripted event.
What gripes i have with Xcom Enemy Unknown is, that for example it forces you to go a certain way in science and engineering, besides the story elements themselves. If you don´t rush satellites for most of the states you have a hard time to survive. Same with the interceptors. A bit more freedom and variety would be helpful, especially for a game with such a possible long time motivation. "Enemy within" helps in this respect, but cannot eliminate the weaknesses in full.
Nevertheless they did quite a good job, bringing the alien hunt to the modern age.
So if you like strategy, grab this one 🙂


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I know just enough networking to be a danger to myself and others and really need some advice for how to setup my home / studio network. Any help is very much appreciated...
Network Hardware:
- Asus RT-AC5300 wi-fi router
- Netgear ProSAFE M4100-D12G 12-port gigabit router
- Netgear ProSAFE XS716T-100NES 16-port 10Gb Smart Managed Switch
- Cable Modem connecting to TimeWarner Cable ISP
- Studio/Office where I have a 10Gb server and a few 10Gb workstations / render nodes (and gaming machines come evenings/weekends :). They need to talk fast to each other, get Internet access, provide FTP/HTTP server, and be able to VPN for remote access. I also want wi-fi available here for when working on laptop, etc.
- Media/Entertainment Room where I have several devices (Xboxes, Playstations, Rokus, etc., etc.) that I'd like to wire connect (for speed/reliability) and some over wi-fi (i.e. DirecTV which only does wifi). They need to be able to see each other, but also pull media files that are served from my studio server.
- Firewall needed for all, but esp. for office
So obviously the 10G switch is for the office and the 1G switch is for the media room. Where I break down on deciding what is best is where the Internet connection should feed to and where to put the AC5300 wifi. I could see putting the AC5300 right off the modem and then hang the two switches off of it, and then use it's Firewall? Or would the XS716T be superior / more flexible at the gateway and hang the AC5300 off one of the switches as simply a WAP? And what to use for firewall? Should I run a separate network and mask for home vs office network? VLANs? Those are the questions that are overwhelming my decision making process.
Can anyone tell me if there's one or two clearly best practice ways of configuring this?
Thank you!
PS: If you're wondering why 10Gb, I'm a filmmaker and I work with 4K uncompressed frames all day long, as well as CG rendering. Need high throughput.
2 Replies
Oct 19, 2016 at 1:01 UTC
I love VLANs, but here is not the place for them, unless you want to get into defining what one network can do and the other can't.
I'd set it up as
Internet - cable modem - firewall/wireless - 10gb switch
- 1gb switch
According to the data sheet for the switches, they are not firewalls, just routing switches.
Oct 19, 2016 at 7:28 UTC
I like the idea of logically separating your work and home devices. You wouldn't want your guests being able to access your file server, or other work stuff.


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One of my best memories from my childhood was watching my father get ready for work each day. He always would say, "Don't sleep in and let the day escape you. Wake up and be productive so you can feel rewarded before the sun goes down." I took those words to heart, and I'd like to believe they've guided my work ethic through the years.
But a lot has changed since those days. My father would come home at 5 p.m., and that would be the end of his work day. I find that I get home and usually end up checking email well into the evening, and at times, responding to comments on our social media platforms right before I fall asleep. That's probably not the best practice since we all need to unwind and spend time enjoying the rewards of a productive day, but the truth is, it's the new world we live in that keeps us connected 24 hours a day.
Enter the millennial.
I just came back from my first NRMCA ConcreteWorks Conference, and although there were plenty of educational sessions on industry-specific topics, the one session that kept a constant buzz was Relating to Generation Y, the Millennial Generation, in the Workplace. There's been plenty of talk about our aging workforce, as well as the shortage of qualified workers. And it's no secret that that the millennial generation isn't showing much interest in the concrete and construction industries. So how do we solve the problem?
Nadira Hira offered some insight into recruiting and working with the millennial generation. She brought up five key tips:
- Mission: Millennials want to know the organization they're in is a good one. One that they can be proud of. Tell them how they can contribute to the overall mission of the organization, and even more important for us, the industry.
- Team: Talk about work culture in an authentic and meaningful way. With the consistant digital connectivity, millennials are often alone. Their work environment becomes part of their integrated life, and their coworkers become family.
- Learning: Don't assume they know everything that is obvious to you. Where generations before grew up relying on intuition, the millennial generation depends on the Internet for immediate answers.
- Story: Talk about opportunities at your organization with examples using people, not flow charts. They don't want to know that it will take X amount of years to move up to Y position. Talk about how Joe Concrete started as a driver and how he created his legacy at the organization.
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We must be authentic with how we talk about our industry. Provide an opportunity for this next generation to know that they can be part of something great, and that we are willing to show them how.
What are your thoughts on recruiting and working with the millennial generation? What are some of the obstacles you have come across? I'd love to hear from you.


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RevoStock Text And The City 348438
After Effects Project \ High Energy \ Lights \ Particles \ Urban
Text And The City 348438 by MartinProUser @ RevoStock
After Effects Project \ Commercials \ Intros \ High Energy \ Lights \ Particles \ Urban
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Required Software: This product is an Adobe After Effects project file. It requires Adobe After Effects version CS4 or higher as well as any plug-ins listed follow. Required After Effects Plugins: Trapcode Starglow
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