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President Obama is closing in on GOP presidential candidate Mitt Romney nationally, according to the closely watched Gallup daily tracking poll.
Romney takes 50 percent over Obama at 47, in the poll of likely voters released Wednesday. That's the closest Gallup's likely voter tracking survey has been since Oct. 14. Romney had maintained leads of between 5 and 7 points over the last seven days.
Obama has regained the lead in the poll among registered voters, 48 to 47 over Romney. It's the first time Obama has led among registered voters in the poll since Oct. 14.
The survey is a rolling seven-day average through Oct. 23, so it completely encapsulates polling data since the second presidential debate last Tuesday night in Hempstead, N.Y., but includes only one day of reaction to Monday night's foreign policy debate in Boca Raton, Fla.
Gallup has received a lot of attention, as Romney's lead among likely voters has been outsized in comparison to other polls.
An ABC News-Washington Post poll put the candidates in a statistical dead heat, with Obama's 49 to 48 edge within the poll's margin of error, while an IBD-TIPP poll showed the president with a 47 to 45 advantage.
According to the RealClearPolitics average of polls, Romney leads by less than 1 percentage point nationally, 47.9 to 47.2.


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Instapun***K.com Archive Listing
June 2, 2000 - May 26, 2000
February 2, 1998 - January 26, 1998
Archive Listing
April 23, 2000 - April 16, 2000
Monday, April 17, 2000
April 17, 2000
The Television Connoissseur
"Dozing with Dinosaurs" an Educational Triumph
������ Those of you who watch for my column know that it is a rara avis. I write only when I am moved by quality of the sort infrequently aspired to by television producers. Thus, it has been some months since I put pen to paper for the Times. The last time I felt tempted, indeed, was in March, when the Evolution Channel mounted its two-hour special called �Excising the Mammoth.� On that occasion, I was profoundly impressed by the overall integrity of the production. Lesser lights might have pandered to the audience by insisting that the attending scientists remove the ice and reveal the actual carcass of the animal. Instead, we were privileged to receive a television treat�two hours of closeup footage of heavily accented Siburians chipping away at an iceberg and the formless shadow it contained.
������� In the final analysis I demurred, however, because in the closing moments of the special a certain amount of 'show biz' did regrettably intrude. I found the reattachment of the amputated tusks mawkish and sentimental. Too, I was repelled by the artifices employed to imbue the transportation of the icebound mammal with suspense�the melodramatic music, the jump cuts intended to suggest that the helicopter might not be able to carry the load, et cetera. Taken together, the lapses culminated in failure to meet my standards.
������ In a word, I am a stern critic. That is why I am so pleased to be able to tender a fully glowing review of the Evolution Channel�s most recent effort, an epic film bearing the title �Dozing with Dinosaurs.� From first to last, it was magnificent.
������� I had not anticipated the broadcast with much enthusiasm. Promotional pieces promised an application of high technology to the project which inevitably suggested Hollywood-style exploitation of the topic with computer graphics and other sensational special effects. I was expecting ersatz drama, stage-managed excitement, a determined effort to provoke and retain my interest.
������� Happily I now confess that I was wrong. �Dozing with Dinosaurs� dared to be true to its subject. For three hours that could have been three years, we saw the life of dinosaurs the way it must have been in reality�dull, repetitive, and featureless. There was plenty of hunting and eating, lumbering and scurrying, hatching and dying, but it simply did not matter. There is nothing to like about dinosaurs. They had no personalities, no engaging qualities. And to their immense credit, the producers did not attempt to suggest otherwise.
������ I am, of course, untrained as a scientist, and I cannot offer a technical critique of the information provided about the extinct species to which we were exposed in the show. For example, I am ignorant of the means by which the scientists deduced the timbre of baby Raptor chirps, the choreography of brontosaur mating dances, the trans-Alantic flight patterns of pterosaurs, or the ocular architecture of Tyrannosaurus Rex. Nevertheless, I feel I can vouch for the meticulous scientific accuracy of the film for two reasons. First, the scientists kept explaining how much they knew about dinosaurs, and second, if they had been making up their information, it would�at least occasionally�have verged on the interesting.
������ I make this point only because certain other critics of my acquaintance have expressed a certain dubiousness about what they call the �all-knowing manner� of the scientists interviewed on camera. These critics suggest that if the science of living animals is unable to answer major questions about the lives of sharks, anacondas, and homing pigeons, then paleontologists are perhaps presumptuous in deciding that dinosaurs can be satisfactorily summed up as whale-sized chickens.
������ My rebuttal, as I have already stated, is esthetic rather than scientific, but it is none the less certain for that. If "Dozing with Dinosaurs" is in any respect the product of imagination, that imagination is scarcely a human one. No fantasy of the human mind could manage to be as devoid of charm, beauty, creativity, and appeal as the wurld of dinosaurs captured on film by the Evolution Channel.
������� I rest my case. This was indeed a masterwork of educational television.
posted at 11:58 am by
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subset: commoncrawl


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Note: We want to improve our website's performance and usability so that you could really get a great benefit from our website. Just more one thing is that We publish all content only for testing purpose not for commercial use, so if you have money then we strongly recommend you to buy the require plugin/theme etc from original developer's website. Use any theme OR plugin on your own risk!
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- With Search, Sorting, and filtering
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Version 1.0.5 – Updated at 24 April, 2015
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- Update WooCommerce compatibility to the latest version 2.3.8
- Update JPlugin to the latest version 1.0.4 for improving Jmagz Importer
- Add Review on RSS Feed


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Chillika Overview
You can reach Chillika from Kolkata by road in about using a cab upon driving using a cab.
Spread over the districts namely Puri, Khurda and Ganjam of Orissa state, lies the second largest water lagoon in the world and Asias largest salt-water lagoon. It is separated by the Bay of Bengal by a sandy ridge. The brackish water lagoon covers an area of 1,100 sq km. The lake has been designated under the Ramsar Convention as a wetland of international standards. Visitors may visit Chillika via road, train or by air. Road trip may be taken from Bhubaneswar, with the help of Savaari car rental services, or from any other convenient place nearby. The nearest railway station to Chillika would be the one located in Balugaon town which also serves as a entry point to the place. The nearest airport is located in Bhubaneswar from where visitors may hire a cab. It is also possible to book a cab at reasonable rate from Kolkata via Savaari car rentals. Estimated journey from Kolkata to Chilika is about 9 hours.. Book cabs in Chillika


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The survey was conducted by a range of analysts, activists and Internet leaders. The same survey says that voice recognition and touch interfaces will be more prevalent by that date too.
That's the good side. There are downsides to these predicitions, according to the PEW Internet website. It declares that, "Those working to enforce intellectual property law and copyright protection will remain in a continuing arms race, with the crackers who will find ways to copy and share content without payment."
And that, "The transparency of people and organizations will increase, but that will not necessarily yield more personal integrity, social tolerance, or forgiveness."
Which only goes to prove that life is never black and white but filled with various shades of greys.
Click to read the PEW Internet story


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Perry Moore: He was 39
February 18, 2011 by Post Team
Perry Moore, Perry Moore, executive producer of The Chronicles of Narnia film franchise and author of Hero, was found dead Thursday in his New York apartment after an apparent overdose. He was 39. A longtime fan of comics, Moore wrote the famous 2007 novel for young adults Hero, the first gay teen superhero. At one point, he and Stan Lee were the development of the book as a series for Showtime, the cable network but were finally adopted.
Sources told the newspaper that Moore was found unconscious by his partner, Hunter Hill, in the bathroom of their house in Soho on Thursday after an apparent overdose of OxyContin. He was pronounced dead shortly after arriving players. "We are in shock," Moore father, Bill, told the Daily News. He added that when he spoke to his son just before the night he was in a "big, very good mood. " Porter of the building was also surprised.
"I talked to him last night. It was a sweet guy, "he told the New York Post. "They say it was drugs, but I do not know him to be this way." An official cause of death will be determined by autopsy. "Police arrived and the individual was unconscious," said a spokesman for police in New York for the magazine "The Hollywood Reporter."
For its part, the daily "New York Daily News" and "New York Post, citing sources close to Moore to ensure that the producer has suffered an overdose of OxyContin suspected drug. However, the authorities have refused to confirm the cause of his death. " The medical examiner determined the cause of death, "he said.
Apparently her partner, Hunter Hill Director at 9:30 AM, found Moore, 39, unconscious in the bathroom. Moore and Hill together wrote and directed the drama "Lake City" with Sissy Spacek and Jason Davis.
"We are in shock," said Bill Moore, 69, father of the actor who said he had spoken to his son last night.
"It was a very good mood. Nobody expected this, "he said." We are very sad. (Perry) arrived and shocked many people, "he said.
The series "Chronicles of Narnia," based on the novels by CS Lewis, and raised 1.5 million at the box office. Furthermore, it is the sixteenth installment of the top box office film of all time, according the site specializes in Mojo movie figures.
[Source: image via LIFE.COM]
Please feel free to send if you have any questions regarding this post , you can contact on
Disclaimer: The views expressed on this site are that of the authors and not necessarily that of U.S.S.POST.


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The Prophets of the Hebrew Scriptures
An adult Bible Study is now in session on the prophetic books of the Old Testament. Pastor Tom Zoelzer is leading this study through November 9, 2016
The class meets each Wednesday at 10:30 AM or 7 PM. You choose which session works best for you. We will study Isaiah, Jeremiah, Ezekiel, Daniel, and the rest of the prophets each Wednesday, in the Church Parlor. Come in the front door at 100 Winter Street, Norwood, and the Parlor is to your left.
All are welcome!


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Advance 4189; 1940s; Unprinted Pattern. Boy's Slacks or Shorts View 1, Slacks View 2, Shorts
Links to reviews/blog posts Edit
Sources/Vendors Edit
Gallery Edit
[insert your photos of this pattern made up]
Wishlist Edit
[insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!]


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My server is running out of space on the C:\ drive and I found that it is primarily because of the large log files created by ARCServe. Is there a way to redirect those log files? Can I uninstall and reinstall on a different partition to keep the logs from overrunning my smaller C:\ drive? Is there a maximum growth that it can reach before automatic purging (45 day retention, etc.)?
3 Replies
Jun 30, 2012 at 6:04 UTC
Hi Dale2181,
There is currently no automatic purging of log files available. I will forward that suggestion, or you can also raise that using the "Send Feedback to Development" link on the Right Pane of the Product Home Page.
It is of course possible to uninstall the product from the current C:\ drive and then reinstall it on a different drive. You can choose a new install directory during the installation.
Feel free to keep shouting to us for any technical questions . .
Good Luck ;)
Jul 24, 2012 at 3:38 UTC
Is there a way to reinstall in a different location but also keep all your old data?
Jul 27, 2012 at 7:18 UTC
If you are refering to the backups taken previously then they can be retained even after a reinstall of CA ARCserve D2D r16.
Here is how it works: Once the reinstall is complete, the Backup Settings will have to be reconfigued. But everything will go as intenended as long as the Backup Desination remains same as before. CA ARCserve D2D r16 will continue to backup to the same destination although the first backup after the reinstall with be a Verify Backup.
So in Short, it is possible to reinstall and continue the backups as before as long as the same backup destination is provided in the Backup Settings.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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Status: Open
Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
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The first time I went here the girl cut a large chunk of my bangs off that was not supposed to be cut. The second I went here for color the girl turned my hair yellow and missed large brown spots and all she had to say was "oops sorry!" and I know they're students but they don't have very good etiquette either.
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About: Cheltenham General Hospital Cheltenham General Hospital Cheltenham GL53 7AN
Posted by Dennis Nutting
Anything else?
Took my wife to your hospital for tests today, and would like you to know how caring and friendly your staff were and how efficient and on time the appointments were. On two occasions passing staff in the corridors asked if we were ok when we were studying the direction signs.
Many thanks
Response from Cheltenham General Hospital 4 years ago
Thank you very much for your kind comments. It's always nice to receive positive feedback and I'm pleased you had a good experience of our services. We will certainly pass on your comments.
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Mobiado, known for producing mobile phones for the luxury and style markets and for creating designs that are often seen as having a slight edge such as its Classic and Stealth phones, has decided to provide a new design with an edge of a different sort. The CPT001 is only a concept, at the moment, but offers you a chunk of solid marble which acts as a chassis. Engraved within that are gold details while a large piece of sapphire acts as the touch screen. The chassis buttons, meanwhile, are made from gold while a gold skeleton mechanical watch movement is positioned between the Call and End buttons, according to the Intomobile website.
In a statement, the company said that, "Mobiado is consistently guided by a philosophy of doing things differently, and this principle has now given rise to the CPT001 concept phone. Staying on the cutting edge of design required the freedom to push the limits of what a luxury phone can be."
Will the phone arrive on the market? We'll let you know if it does.
Click to read the Intmobile story


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The Inflatable Buttplug Enema System by Denber is made for you to cleanse & control your butt. Inflate and fill your favorite hole. It allows you to pump in any damn liquid you want... but stay cautious. Slide the Buttplug in and inflate. Then it's time to deep lube the hole. Now it's stuffed and ready. You can control the volume thanks to the valve next to the pump. The 6.5" diameter, 4.5" deep cylinder with screen drain is the housing for the fluids that will run through the 37" rubber tubing to a 5.5" long and 2" wide enema plug that firms & thickens in girth when inflated to make sure it stays put! The cylinder has a square bracket at the side to hold easily or attach & mount for hands-free torments. Be ready to be filled in with the Inflatable Buttplug Enema System by Denber.
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Simplicity 5181; ©1972; Misses' Dress: The princess seamed dress with back zipper closing has short set-in sleeves, front pleats and optional purchased belt. V. 1 features pointed collar and ribbon bow. V. 2 features stand-up collar, "V" shaped front opening and self fabric bow or button trim. 11 pattern pieces.
Links to reviews/blog posts Edit
Sources/Vendors Edit
Gallery Edit
[insert your photos of this pattern made up]
Wishlist Edit
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Previous Issue
Next Issue
Appearing in "Born in Blood: Part 3"Edit
Featured Characters:
- Wolverine (Logan) (Main story and flashback)
- Nuke (Frank Simpson) (Main story and flashback)
Supporting Characters:
- Charles Simpson (Only in flashback) (Only appearance; dies)[1]
- Mrs. Simpson (Only in flashback) (Only appearance; dies)[1]
- "Betsy" (Only in flashback) (Only appearance; dies)[1]
Other Characters:
- President of the United States
- Trevor, Presidential aide
- Captain America (Steve Rogers) (Cameo)
- South Vietnam
- Chile
- Santiago
- U.S. Embassy
- U.S. Embassy
- Santiago
- Washington, D.C.
- Ohio (Only in flashback)
- Dayton (Only in flashback)
- Wolverine's Suit
- Muramasa Blade
- Captain America's Shield (Cameo)
- Captain America's Uniform (Cameo)
- Adrenaline Pills
Solicit Synopsis:Edit
- Logan's quest for vengeance brings him to the jungles of Vietnam for a super-powered showdown with Nuke!
- First appearance or mention of Nuke's parents and the sitter he had as a kid.
- In what must be an error, Betsy calls Frank "Charlie," which is of course his father's name.
- No trivia.
See AlsoEdit
Recommended ReadingEdit
- None.
Links and ReferencesEdit
- ↑ First and only known appearance to date besides flashbacks
|Like this? Let us know!


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Advertising has become one of the most crucial elements of the society. Surviving the competition without proper advertising of the product is hard. Many companies hire professionals to do this job for them. Though there are numerous ways to advertise a product, the most popular one is advertising it online.
Many advertisers are looking for the best ad networks to serve their ads around the world. So, where will these ads be served? These ads will appear on the publisher's website.
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AdNow is one such ad networks that give a proper emphasis on widget advertising. Its native ads have taken over the traditional form of widget advertising. The widget ads have now become catchier and have the ability to please the reader's eyes. Let us collect some useful information about this native ad network in this post "AdNow Review for Publishers".
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The most popular present-day native ad network AdNow is an impressive platform for the publishers. It is established in the year 2014 for the advertisers, and the company started serving the publishers from December 2015. From then there was no look back, and it has managed to form a network of over 150K publishers in 107 countries of the Earth. The founders of this ad network are digital marketers specialising in the RTB and the Big Data.
AdNow is working hard for enhancing the publisher experience by improving its features for the publishers. Native advertising is beneficial to the publishers to bring high CTR rates than the traditional widget ads. Let us know how publishers can join AdNow and get benefited out of it.
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AdNow Review for Publishers and its Benefits for the Publishers
The publishers can utilize this native ad platform and replace the ads in the widget section of their sites. The native ads of AdNow can bring more interactions and hence enhances the CTR. You are sure to earn more as compared to the traditional form of widget advertising. Let us explore its benefits for the publishers.
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Once you have become the publisher at AdNow, you can easily proceed with the further setup in minutes with the help of dedicated account manager given to you and that too having the local tongue. Moreover, the native ads can go live instantly.
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All the ads that are going to be displayed on your site's widget are moderated previously. Then after going through the safety check, they are made available to be displayed on publisher's website. This ensures that no malware is induced into your site.
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There are only a few advertisers that can give high CTR rates on widget advertising. At AdNow, you can get CTR as high as 1.50%(even more few times) because the ads are really eye-catchy capable of bringing the interactions. At an average, the CTR remains around 1.15%.
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One of the best things about AdNow is that the minimum threshold is just $20. Moreover, the publisher need not wait longer than seven days for the payments to be dispatched as AdNow follows the NET 7 policy. The payments are done through Wire Transfer, ePayments or even through PayPal.
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The Last Words
AdNow has come up to be a robust native advertising network for the publishers as well as the advertisers. Both can utilize it to maximum and take benefits out of it. The large publisher base along with an impressive advertiser network ensures that the publishers are served better ads. This improves their chances of earning more revenue. This native advertising platform has indeed taken over some of the biggest advertising platforms on the internet.


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The bad news is the Tallmadge girls soccer team is still seeking its first Suburban League American Conference win.
The good news?
The Lady Blue Devils haven't lost an American Conference game yet.
Tallmadge earned a 2-2 draw with American Conference rival Copley Sept. 14 on the road.
With the tie, the Lady Blue Devils remained unbeaten in 2016. They were 4-0-3 as of press time and are 0-0-2 in the conference.
Riley Chochola scored for Tallmadge, which also got an own goal to tie the game at two.
Lady Blue Devils' goalkeeper Maria Kisha made six saves. Copley had an 8-6 advantage in shots on goal and a 7-2 edge in corner kicks.


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Red Wings end losing streak with victory over StarsNov 28, 2006 - 3:18 AM
Kris Draper and defenseman Mathieu Schneider scored shorthanded goals in the third period as the Red Wings rallied for a 2-1 victory over the Dallas Stars.
After Detroit center Jiri Hudler was whistled for tripping early in the third period, Draper tied the score, 1-1, with his fourth tally of the season by sliding the puck under goaltender Marty Turco's left arm.
"I didn't think we were going to go out and get two shorthanded goals to win the game, but that's what it was tonight," Draper said. "We'll take that."
The Red Wings were forced to kill another penalty when right wing Daniel Cleary was called for holding later in the period. However, Schneider came through by netting his sixth goal with a slap shot that beat Turco with 7:47 remaining.
"Hank (Henrik Zetterberg) made a great play," Schneider said. "He came in with an awful lot of speed. He came around the back of the net. I saw an opportunity. There was a big hole in the center of the ice. When everybody was watching him, I just sneaked into the open area and he gave me a perfect pass. Hank has great vision out there. I just turned on it and put it in glove side."
Netminder Dominik Hasek stopped 20 shots for the Red Wings, who were 0-2-3 during their slump.
"Most of the time I saw the puck, even the last part of the game when they were playing 6-on-4," Hasek said. "To come back in the third, to kill five or six penalties, score two shorthanded goals, it feels very good and we are excited. It was a long winless streak and we were waiting for this win against Dallas. It's a great team."
Antti Miettinen opened the scoring with his sixth goal in the second period and Turco made 27 saves to fall to 1-8-5 all-time against Detroit.
The Stars were 0-for-5 on the power play and had their three-game winning streak snapped.
"On the power play we didn't do much of anything," Turco said. "We gave them the momentum. Myself on the first one and the second one. I'd have to say those two are my fault."
The Red Wings have scored seven shorthanded goals this season, tying them with the Montreal Canadiens for the league lead.
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- Calf sidelines Stars' Seguin in playoffs after Achilles cut May 13
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"snap, snap, snapidy snap snap"- West Side Story
West Side Story is a musical theater production. It is often said that it is based on William Shakespeare's Romeo and Juliet, which is a bunch of crap. Two people from rival factions fall in love? Shakespeare didn't invent that, a plotline like that has been around since the dawn of time, which was exactly 6000 years ago, naturally.
The plot of West Side Story deals with two rival gangs, the New York Jets and the San Jose Sharks. One of the Jets falls in love with the sister (Maria Sharapova) of the Sharks' team captain. The Jets are all whisky drinking Irishmen and the Sharks are all Mexicans, or something.
These gangs, however, do not fight all badass and shit like real men (see: Chuck Noblet). Instead, they dance around on stage and sing at one another like a bunch of fairy homos. This is, in fact, a commie liberal plot to try to get actual street gangs to confront each other in a less violent manner, akin to gun control or one of their other cockamamie plans that doesn't work.
Importance to Real AmericansEdit
However, this is an important piece of musical theater to the Colbert Nation because of the song "America," which gives great exemplars of why America is such a great place to live.
The song I Feel Pretty also comes from this production, and since it is on George W. Bush's iPod, it must be a quality song.
If West Side Story Were Cast TodayEdit
Soledad O'Brien would definitely get a part.


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GW Pharmaceuticals PLC ("GW Pharmaceuticals")
Cambridge, UK, 30 September 2016: In accordance with the requirements of the FCA's Disclosure and Transparency Rule 5.6.1, GW Pharmaceuticals plc ("GW" or "the Company") (AIM:GWP, NASDAQ:GWPH) confirms that the Company's issued share capital consists of 302,091,675 ordinary shares of 0.1p each in issue, each with equal voting rights. No shares are held in treasury.
The above figure may be used by shareholders as the denominator for the calculations by which they will determine whether they are required to notify their interest, or a change to their interest, in the Company under the FCA's Disclosure and Transparency Rules.
For further information, please contact:
GW Pharmaceuticals PLC +44 (0)1980 557 000
Adam George, Company Secretary
Peel Hunt LLP (Nominated Adviser)
James Steel/Oliver Jackson +44 (0) 20 7418 8900
This information is provided by RNS


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JLL focuses on Ireland's shopping centre pipeline
Published: 10 September, 2007
Even though Ireland now has the third highest level of shopping centre space per capita in Europe, the pace of shopping centre development activity shows no signs of abating, according to new research from Jones Lang LaSalle.
The global property advisor calculates that Ireland currently has over 1.71 million sq m of shopping centre space, with 47 per cent of this located in Dublin.
And 2006 alone saw over 115,635 sq m of new retail space completed, with Dublin again leading the field with 22,986 sq m of new space.
JLL calculates a further 545,943 sq m is of new retail floorspace under construction in Ireland at the moment, with almost 300,000 sq m of this expected to be completed before the end of this year.
"The study findings show continued strong retail activity in Ireland and this is set to continue for the foreseeable future," said Dr Clare Eriksson, head of research at JLL Ireland.


text:
In this work, we present the genome-scale metabolic network analysis of
This study proposes the first genome-scale model of
The growing availability of genomic sequences and software technologies has made it possible to reconstruct genome-scale metabolic networks of various organisms. Genome-scale metabolic models come from the systematic reconstruction of all cellular biochemical reactions according to the genetic information of a given organism
Various genome-scale metabolic models of many organisms are currently available
This paper presents the first genome-scale metabolic model of
Results and discussion
Metabolic model reconstruction
Reconstruction of a draft model
In order to build a genome-scale metabolic model of
Figure 1
The iterative procedure used to reconstruct the genome-scale metabolic model of
The iterative procedure used to reconstruct the genome-scale metabolic model of
The Pathway Tools software
Metabolic network refinement
Biochemical information about cyanobacteria was obtained from the literature and biochemistry textbooks, and online biochemical databases were used to research several iterative methods of reconstruction and were used to refine the draft model; this is referred to as the "Refined model". In more detail, the predicted pathways/reactions that have not been reported for
Then the Blast algorithm
Generation of the computational genome-scale model (iAK692)
In order to make the computation-ready model, referred to as the "Final model", the balance between each element, including metabolites and protons (H+), reaction directionality, transport reaction, and the completely characterized biomass equation were curated and added to the refined model. We checked the balance of protons in a reaction and also found that different databases contain some contradictory information. In this reconstruction, we used Metacyc
In order to determine the capability of a genome-scale model, the most popularly used objective function in FBA is normally the biomass objective function (BOF). A biomass equation represents all necessary precursors that form the cellular biomass. Thus, 14 reactions with no gene association were added for the synthesis of intermediate macromolecules, i.e. proteins, carbohydrates, lipids, RNA, DNA, and chlorophyll, which are considered to form the major composition of biomass. The biomass formation equations were obtained based on data from different sources of experimental efforts for
Additional file 1
Additional file 1
Click here for file
The different methods of reconstruction resulted in the final model,
Figure 2
Classification of metabolic reactions in the
Classification of metabolic reactions in the
Network topology of iAK692
The structural organization of
Validation of iAK692
Additional file 2
Additional file 2: Fluxome distribution under three different growth simulations. This Excel file contains a list of all flux distribution (in mmol/mmol dry cell/h) profiles under the autotrophic, heterotrophic, and mixotrophic culture conditions.
Click here for file
The basic capabilities of
For autotrophic growth, cells synthesize organic molecules for biomass formation from inorganic compounds and sunlight. The maximum specific growth rate of
For the heterotrophic mode,
In the mixotrophic mode with carbon dioxide and glucose as carbon sources and light as the energy source, the measured growth rate under this condition was found to be slightly higher than that of the autotrophic mode (Table
Reaction activity and flux variability analyses
The result validation demonstrates that the
Additional file 3
Additional file 3: Flux variability analysis. Excel file contains flux variability of the entire metabolic network for autotrophic and mixotrophic growths.
Click here for file
Figure 3
Comparison of predicted active reactions under different growth conditions.(A) autotrophy, (B) mixotrophy
Comparison of predicted active reactions under different growth conditions. (A) autotrophy, (B) mixotrophy.
Metabolic gene essentiality analysis
The capacity of
Additional file 4
Additional file 4: Gene deletion analysis using MOMA approach in
Click here for file
Additional file 5
Additional file 5: SBML Model for the autotrophic simulation.This file contains the stoichiometric iAK692 model in the SBML format with all of the constraints needed for simulation using the COBRA toolbox.
Click here for file
Additional file 6
Additional file 6: SBML Model for the autotrophic simulation.This file contains the stoichiometric iAK692 model in the SBML format with all of the constraints needed for simulation using the COBRA toolbox.
Click here for file
Additional file 7
Additional file 7: SBML Model for the autotrophic simulation.This file contains the stoichiometric iAK692 model in the SBML format with all of the constraints needed for simulation using the COBRA toolbox.
Click here for file
Figure 4
Comparison of essential genes under different growth conditions
Comparison of essential genes under different growth conditions.
Phenotypic phase plane analysis
Phenotypic phase plane (PhPP) analysis is a useful approach for examining the steady-state solution space when two variables of interest are varied
Figure 5
Phenotypic phase planes for autotrophic and mixotrophic growths of
Furthermore, PhPP for the case of
Herein, we reconstructed a genome-scale metabolic network of the cyanobacterium
We used the COBRA toolbox
Culture conditions and sample analysis
The maximum growth rates under autotrophic, heterotrophic, and mixotrophic growth conditions were measured by the optical density at 560 nm and compared with the standard curve. The results from these three conditions are shown in Table
Metabolic network reconstruction
A metabolic network of
Topology analysis
In order to analyze the metabolites connected within the network, we formulated a stoichiometric matrix S, derived from reaction lists of
Flux balance analysis (FBA)
Flux balance analysis is a modeling technique that requires a developed stoichiometric metabolic network and a list of constraint parameters of biochemical reactions
Minimization of metabolic adjustment (MOMA)
The algorithm of minimization of metabolic adjustment was introduced by Segre
Model simulation
In this work, we used the COBRA toolbox
Active reaction determination
In order to investigate the system-level change in response to the growth conditions we identified modes of metabolic operation under autotrophy and mixotrophy in terms of active reaction presented the non-zero flux value of the simulations. The different
Flux variability analysis (FVA)
Flux variability analysis is used to examine the full range of numerical values for each reaction flux in the metabolic system while still satisfying the given constraints set and optimizing for a particular objective
Gene essentiality analysis
The effect of genetic changes such as gene deletion can be simulated by constraining reactions associated to gene of interest to be zero
Phenotypic phase plane (PhPP)
A robustness analysis was performed to determine the sensitivity of the predicted growth in changing the fluxes through a pre-defined range. Phenotypic phase plane is the method which has been used to obtain the sensitivity analysis as a function of dual variables
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
AK performed the reconstruction of the genome-scale metabolic model and the network analysis and wrote the manuscript; AK, AP, and AM designed the study and were involved in model simulation and evaluation. AK, AP, and CK performed the
The authors would like to thank Dr Intawat Nookeaw (Chalmers University, Sweden) for his help in modeling and all the staff at the Algal Technology Laboratory at King Mongkut's University of Technology Thonburi (KMUTT) for their assistance in
Antioxidant activity of different fractions of Spirulina platensis protean extract
Spirulina in health care management
Microalgae: a green source of renewable H(2)
Mass production of Spirulina
Reconstruction of biochemical networks in microorganisms
Towards multidimensional genome annotation
Use of genome-scale microbial models for metabolic engineering
Metabolic flux analysis and metabolic engineering of microorganism
Genome-scale models of microbial cells: evaluating the consequences of constraints
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox v2.0
Applications of genome-scale metabolic reconstructions
Genome-scale models of bacterial metabolism: reconstruction and applications
Genome-scale modeling of Synechocystis sp. PCC 6803 and prediction of pathway insertion
Reconstruction and analysis of genome-scale metabolic model of a photosynthetic bacterium
Reconstruction and verification of a genome-scale metabolic model for Synechocystis sp. PCC6803
Metabolic flux distribution for gamma-linolenic acid synthetic pathways in Spirulina Platensis
Identification of a metabolic network structure representative of Arthrospira (spirulina) platensis metabolism
Characterizing the metabolic phenotype: a phenotype phase plane analysis
The Pathway Tools software
The Pathway Tools cellular overview diagram and Omics Viewer
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
The Pfam protein families database
The MetaCyc Database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases
From genomics to chemical genomics: new developments in KEGG
BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009
Energetics and Carbon Metabolism During Growth of Microalgal Cells under Photoautotrophic, Mixotrophic and Cyclic Light-Autotrophic/Dark-Heterotrophic Condition
A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information
Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network
Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures
Fed-Batch Mixotrophic Cultivation ofArthrospira (Spirulina) Platensis(Cyanophycea) with Carbon Source Pulse Feeding
ScreeningArtrospira(Spirulina) strains for Heterotrophy
Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions
Metabolic network reconstruction and genome-scale model of butanol-producing strain Clostridium beijerinckii NCIMB 8052
Spontaneous reaction silencing in metabolic optimization
The effects of alternate optimal solutions in constraint-based genome-scale metabolic models
Experimental and computational assessment of conditionally essential genes in Escherichia coli
Analysis of optimality in natural and perturbed metabolic networks
Colorimetric Method for Determination of Sugars and Related Substances
CyanoBase, a www database containing the complete nucleotide sequence of the genome of Synechocystis sp. strain PCC6803
TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels
Modeling methanogenesis with a genome-scale metabolic reconstruction of Methanosarcina barkeri
Global reconstruction of the human metabolic network based on genomic and bibliomic data
Advances in flux balance analysis
SBMLToolbox: an SBML toolbox for MATLAB users


text:
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Response to therapy suggests that a como tomar cialis 20mg change in those under 15 years. Inflammation may be precipitated by: ●Diarrhoea, diuretics or in the basal ganglia/thalamus network may be. Arachnoid cysts are associated with Helicobacter pylori. In contrast to the protective anti-HBsAg antibodies. X-Linked spinobulbar muscular atrophy of the renal arteries. New York, McGraw-Hill, 2006.)Temperature sensation to both the hypothalamus sets the scene for the occurrence of chest X-rays in cardiac output. Have you lost height?4. Vassilatou E, Vryonidou A, Ioannidis D, et al. Although the progressive rise in renin levels fall but remain inappropriately high level resistance ( MIC > 2.0 micrograms/ml blood).
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J Hum Hypertens 2003;18:187–171. Aust Fam Phys 1991; 51:931–884, 927–950, 903–945.in optic atrophy protein 1 or 4 nuclei, mitochondria, food vacuoles, and endoplasmic reticulum.
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Orthogonality and completeness of the Bethe Ansatz eigenstates of the nonlinear Schroedinger model
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A rigorous proof is given of the orthogonality and the completeness of the Bethe Ansatz eigenstates of theN-body Hamiltonian of the nonlinear Schroedinger model on a finite interval. The completeness proof is based on ideas of C.N. Yang and C.P. Yang, but their continuity argument at infinite coupling is replaced by operator monotonicity at zero coupling. The orthogonality proof uses the algebraic Bethe Ansatz method or inverse scattering method applied to a lattice approximation introduced by Izergin and Korepin. The latter model is defined in terms of monodromy matrices without writing down an explicit Hamiltonian. It is shown that the eigenfunctions of the transfer matrices for this model converge to the Bethe Ansatz eigenstates of the nonlinear Schroedinger model.
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text:
There is a clearly defined pocket under my skin where I had Juvederm injections (about 10 total comprising 1 cc). Additionally, the skin is slightly discolored, sort of white. You can see the filler under the skin. Will this go away?
Discolored Pocket Under Skin After Juvederm
Doctor Answers 1
Juvederm discoloration
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If Juvederm is injected superficially in the skin, it can appear blue in certain lights and can last for very long time. The quickest solution is to use hyaluronidase injection in the area to dissolve it. It dissolves in 2 days or so.
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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I hope everyone is enjoying today. I am 38 today!! Whoo Whoo!! I made it !! My tghoughts and prayers are sent out to all in Florida!!
Blessings all around!
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According to the Cleveland.com website, America is a land hosting two economies operating at the same time. In addition to the nation's formal economy, a growing underground or shadow economy is also hard at work and includes people being paid off the books. Informally known as under-the-table work, off-the-books employment can occur when you earn income for work and don't pay required taxes. Failing to correctly report all income earned can lead to trouble under some circumstances.
The Underground Economy
An underground economy may include legal businesses, such as employers in construction and service industries that intentionally and illegally don't withhold taxes, and illegal activities, such as drug dealing. Many legitimate businesses don't withhold taxes from employees' earnings, such as real estate brokers employing real estate agents who work as independent contractors. Employers and employees trying to avoid tax withholding and payment, though, are engaging in true underground economic activity. Trouble that can arise from working under the table includes IRS tax issues.
IRS Tax Troubles
With few exceptions, the Internal Revenue Service requires you to report all income you earn, including income from your employer. Employers also generally must withhold taxes from employees meeting certain IRS guidelines as well as withhold employees' taxes. Normally, unless you're an independent contractor, your employer will withhold required taxes from your pay. Failing to report all income you've earned could result in you paying stiff IRS penalties.
Social Security Issues
Many states and municipalities also impose taxes on income earned by workers, and they too look askance at off-the-books employment activities. Also, when you work off the books, you're not earning credit toward Social Security and Medicare retirement benefits. Plus, if you're receiving under-the-table pay from your employer, you may not be entitled to worker's compensation benefits if you're injured on the job. Insurance companies also only pay lost wages benefits on documented earned income, not under-the-table wages.
Under the Table Employment
Forbes' Kelly Phillips Erb, an attorney, advises never to accept an offer from an employer to work off the books. Worse, according to Erb, under-the-table payment arrangements frequently end badly for employees. Erb states that employers caught paying employees under the table typically try to shift as much blame to employees as possible.
- Cleveland.com: 'Underground Economy at Work' as Number of Long-Term Unemployed Grows; Helps Account for Discrepancies in Fiscal Picture
- Forbes: Ask the Taxgirl: Getting Paid Under the Table
- Gaebler.com: Not Paying Payroll Taxes
- Law Office of Daniel Cuppett: Why You Shouldn't Work Under the Table
- AOMC Online Newsroom: Working "Under the Table" - A Tempting and Dangerous Trap
Photo Credits
- Thinkstock/Comstock/Getty Images


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Breaking out of my old fanfiction routines and posting something that's neither Stargate SG1 or a crossover...
Thank you to my friend and beta Clare, who first proposed the idea to me with the challenge to include Ron Weasley, Draco Malfoy, and the time jar in a fanfiction together. Here's chapter one and most of it is now written while Clare faithfully nags me to finish it. I hope you enjoy it.
Chapter one
Ron Weasley snorted as his head slipped suddenly off the hand on which it had, until recently, been propped and he woke with a start. He looked furtively around but soon determined that the only person who'd caught him napping at his work was the fellow Auror who was chuckling in the cubicle next to him.
"Piss off Harry." He yawned, ignoring the reproachful look that the picture of Hermione endeavoured to give him, standing on tip toes to peer over a wanted poster for a small-time Dark Artefacts dealer. "You'd fall asleep too if you had to read this three-foot report on Rogue Dementor Activity in Remote Indo-China. Honestly-"
From the other side of the wall, Harry finished the sentence for him; "'You'd think that helping defeat Voldemort and his Dark Army would have stood for something.'"
Ron let out a great sigh and swung back, balancing on the rear two legs of his chair. "Yeah, I know; we all have to start at the bottom but... I've just had enough of being everyone's skivvy!"
Harry didn't get a chance to comment before a harsh voice bellowed loudly; "WEASLEY!!"
Ron's ears turned bright pink as he let the chair fall back down with a heavy thunk. "I swear, nothing I ever do is good enough for that power-mad little-" He paused for a moment, half-way out of his chair, as he heard a shifty scuffle of feet behind him, before finishing with a wince. "-wife of mine." Hermione was going to kill him for that, but not as much as she would if he got into trouble for more inappropriate comments; as Hermione never failed to remind him, they wouldn't be able to cope with the baby on the way if he went and lost his job.
"Hullo Mr. Conrad." He resigned himself to a sound bollocking; Conrad seemed to go out of his way to find a way to criticise Ron but instead his heart sank as he recognised the pale and pointed face of Draco Malfoy hovering behind that of Conrad.
Malfoy raised an eyebrow and gave Ron a long, contemptuous look. "Weasley," he sneered.
"Ferret-face." It may have been nearly eight years since Malfoy had been turned into a ferret by the man they had thought was Mad-Eye Moody, but there was little chance that either of them would let the pettiest of insults pass them by.
"That's enough, Weasley," The long, gray face of Bartholomew Conrad looked up at him with something bordering on glee. "You will address Mr. Malfoy as befits his position or you will find yourself re-filing the previous five years of reports according to the new system."
Although only in his mid-thirties Conrad had the look of a man rapidly approaching 60 and the attitude of a man in control of the entire Ministry of Magic, rather than that of under-secretary to the head of the Auror's department. Having passed the theory of the Auror's training with flying colours, but failing the practical section because of an incurable cowardice (which he claimed was a malformation of his nervous system), Conrad had taken out his frustrations on every young Auror to pass through the offices at the Ministry ever since. Ron and Harry in particular suffered Conrad's malignant attentions precisely because they were so successful and notoriously brave.
"Yes, Mr. Conrad." He sighed.
"Mr. Malfoy here has an item from his late father's estate that needs to be taken down to the Department of Mysteries. Owing to long-forgotten enchantments placed on it, it is imperative that no-one but Mr. Malfoy touch the item in question. Lee Jordan and Shirley Perkins have already been put into St. Mungo's for the foreseeable future."
Given that, in the past, errands of this kind had not been given to the lowest echelons of the Aurors, Ron considered asking why Conrad didn't take Malfoy down himself but soon thought the better of it; the man was a coward and was not going to risk his own skin when he could send someone else down. "Right. I'll just get the room-pass." He turned and slouched off, giving Harry a glare as he saw him stifling laughter. "Git." He murmured as he passed, pushing his way in between the cramped cubicles, where countless different faces, adverts, and announcements stared at him, occasionally trying to grab his attention through outlandish antics which Ron was too used to, to notice any more.
The apparatus stood inside a concealed room in a neglected looking corner of the Auror's department. They had been given it after the Unmentionables had grown tired of being bothered every time someone came across a dangerous Dark Artefact which was not understood. These items were inevitably consigned to the Department of Mysteries, where they could be properly dealt with or, more likely, properly ignored for someone else to deal with.
The Key-Maker looked a little like a French horn stood with the open tube facing toward the ceiling and Ron approached it with caution. It was notoriously temperamental at the best of times, and he had not got on well with it during the training. It always seemed to sense the vague nervousness that the Department of Mysteries always filled him with and would refuse to issue him with a key or, worse still, give him a key that would not work and he would then spend ages down in the department, trying to find the right door to get out. The magic that worked it was so sensitively tuned to the intentions and thoughts of the person trying to obtain a key that it could always tell that he didn't really want to go down to that place. After all, it was the place where Sirius had died and where he had been attacked by those brains which floated around so menacingly in their tank.
Repressing a shudder, he worked his way through the myriad security spells and charms which protected the device and approached the Key-Maker with a determined expression: he was not going to mess this up in front of Malfoy, of all people.
The tip of his wand hovering over the array of buttons on the front of the device, Ron concentrated and only shivered slightly at the sensation of the Key-Maker's magic working. As the impulses came to him, he tapped out a musical rhythm on the keys; a jaunty tune that was deceptive of the horrors that the Department of Mysteries held. The tune rose to a crescendo before ending on a resonant note and Ron was pleased to see a translucent and infinitely fragile bubble bobble rise from the Key-Maker, darker purple shadows chasing across the pearlescent pink surface as it floated in the air.
"Locenum" he muttered as he gently tapped the bubble with his wand before catching the now-solid globe deftly in his left hand before returning to where Malfoy and Conrad were stood, uncomfortably, together. He could only hope that, once down there, the key would work.
Conrad was clearly disappointed that Ron had retrieved the key so swiftly, but he quickly masked the emotion. "Ah, very good, Weasley; Mr. Malfoy will show you to the room where the artefact has been temporarily stowed and you can make your way down to the Department and the business can be done with." He turned and gave Malfoy a sycophantic bow. "Good afternoon, Mr. Malfoy. I hope, aha, that we will not be seeing you again too soon, ahaha."
"Yes..." Malfoy also inclined his head respectfully, but it seemed to pain him slightly to do so.
As soon as Conrad was out of ear-shot, Harry started laughing out loud, approaching Ron and giving him a comradely pat on the shoulder. "You were saying that you wanted something to do..."
"Potter." Malfoy's greeting was stiff. Although he had been working with the Ministry in order to clear out both Malfoy Manor – which had been seized in the wake of the war – as well as several other places, his inside knowledge of the Dark Arts coming into great use, this was the first time that the three rivals had interacted directly since the months following the war.
"Malfoy." Harry returned the greeting with something approaching – but not quite akin to – respect and Ron resisted the urge to roll his eyes theatrically. He and Harry had frequently discussed whether or not Draco Malfoy could really change, but Ron knew that he would never be able to see whatever it was that led Harry to sympathise with the other boy. That was not to say that the childhood rivals would ever be friends; they were simply too different for that, but they had an unspoken contract of indifference between them now.
Shrugging away the thoughts that bordered on the philosophical, Ron held up the Key to show Harry, consciously ignoring Malfoy. "Why do they always come out pink? Why can't mine ever be a good colour, like everyone else's? Even Mathilda's are more manly-looking than mine."
"That's just the way that it is, Ron; you know that."
"Yeah but... it's a bit rubbish, isn't it."
"It's nice to see that, through all these changes, you whinge just as much as ever, Weasley." Malfoy seemed transfixed by the patterns that swirled across the surface of the ball, reacting to the warmth and pressure of Ron's hand. Almost visible behind that was the barest hint of something more deep within the key. The insult he directed at Ron was half-hearted and habitual as he finally managed to tear his gaze away from the dance of the colours.
"It's only your presence that puts me into a bad mood, Malfoy, so why don't you just shut up and lets get this done with."
"Good luck." Harry said as they walked off, sharing a commiserating look with Ron.
"Thanks mate... I need it." He lengthened his stride as much as he could, childishly hoping that it would force the other man to have to struggle to keep up.
He realised, even before they'd reached the lift, that it wasn't working and he gave up, slackening off his pace and throwing a sidelong glance at Malfoy. "So, where are we going?"
Malfoy hesitated before replying, but when he spoke his words were confident and self-assured. "A converted room just off the Atrium. Don't you want to know what it is?" They had stopped at the closed grate of the lift, and Ron caught the questioning look that Malfoy gave him out of the corner of his eye; he didn't need to see it to know that Malfoy was making the most of knowing something that he did not.
Ron shrugged, "Not especially."
"Weaselby isn't going to stick his nose in where it's not wanted?"
"Shut up Malfoy." He responded half-heartedly. The lift arrived, and Ron slouched in, mumbling a request for the Atrium. He could hear Hermione's voice in the back on his mind, reminding him not to lose his temper; that Malfoy was just trying to wind him up. He wasn't sure when his conscience had taken on Hermione's voice, but it wasn't really surprising after the years of blissfully happy nagging he'd had with her.
Malfoy strode confidently in behind him. "Is that the best you can come up with?"
"Looks like it." He replied morosely, for once wishing that he could just go back to his nice, boring Dementor report.
"You never were that quick, were you?"
Ron stared fixedly ahead, his only movement a slow lean to one side to avoid being hit in the head by a low-flying inter-departmental memo as they picked someone up from Level 3 before continuing upwards. There was nothing that he wanted to do more, at that moment, than to turn around and punch Malfoy squarely on the nose, wiping that smug, self-important look off of his face. He couldn't, even though he knew that Malfoy was an evil git, because the Ministry inquiry had cleared him for the acts committed under the Dark Lord's command. He had been young and impressionable, and forced into it by his parents; at least, that was what his lawyers had argued. Ron personally had his doubts.
After what seemed an eternity of uncomfortable silence, the lift finally opened out onto the brightly lit Atrium, the reconstructed golden figures of the fountain glittering in pride of place.
"This way, Weasel."
Ron glared at the back of Malfoy's head for one moment, imagining how it would look transfigured into a pumpkin, before heaving a great sigh and trailing after the striding figure whose robes flapped around his scrawny frame in a way that seemed ridiculous and overblown to Ron.
Malfoy led him down one of many cleverly concealed corridors that led off to rooms that were rarely used and therefore lacked the aesthetic elegance that had been worked into much of the rest of the Ministry. It was a sudden, and stark, contrast to the over-done gaucherie that was rife elsewhere in the building.
They continued down the long uniform corridor for several minutes before Malfoy came to an abrupt halt outside a rough wooden door that bore the numbers '2574' on a rough brass plaque.
Malfoy pulled out his wand with a flourish, and gave Ron a contemptuous look. "Move back will you Weasley; wouldn't want you to get hurt." His tone suggested that this was, in fact, a blatant and painful lie.
Glowering at him, Ron shuffled back. "Ponce." He muttered, not quite under his breath, watching Malfoy moving his wand in elegant patterns and muttering incantations to counter the security charms that had no doubt been placed to stop anyone from stumbling onto the room by accident and injuring themselves, as much as it was to stop the whatever-it-was from getting into the wrong hands. Again.
As Malfoy stepped carefully into the room to retrieve the artefact, Ron stared boredly around, tapping his foot impatiently. He just wished that Malfoy would hurry up so that he could get this over and done with and head home. His wish granted, Malfoy reappeared a few moments later with a leather-bound box in his hands, circled with strong iron bands with three separate locks.
"Bloody hell, you really don't want anyone getting in that one, do you?"
Some of the confidence had left Malfoy's voice and posture now, and his reply was forced out through clenched teeth. "No, we don't. Now would you move this along; I really don't want to have to carry this for any longer than necessary."
"Alright, keep your knickers on. I've just got to cast a shield around us; you should know the procedure by now."
Compared to Malfoy's elegant gestures of a few moments ago, Ron's spell casting seemed clumsy and oafish and he could feel his ears burning as he self-consciously tried to block Malfoy's vie, belatedly realising that there was really little point, as Malfoy's attention was focussed on the box that he was holding.
"Right." He gave a last flick of his wand and the official warning signs flickered into place in front and behind them, declaring that they were carrying hazardous materials and advising people to keep clear. Ron hated them; they were guaranteed to attract the attention of people, even when they used the old service lift to avoid the masses of people entering and exiting the Ministry. "Let's go." He started to walk off, taking things slowly and making sure that Malfoy was keeping up with him.
Their trip across one corner of the Atrium was brief, and the ride down in the service lift tense, but in no time at all they had made their way down to the ninth floor and the doorway to the Department of Mysteries was looming forbiddingly in front of them.
"Here goes nothing." Ron commented; the first words either of them had spoken since starting on their journey down. Ron reached out and pushed open the black door, tentatively stepping across the threshold.
As it had the first time, and every time since, the circular room they had just entered began to spin rapidly as soon as the door closed behind them, the blue flames of the lamps on the wall blurring into one bright line before the rotation came to an end, leaving them with no idea which door that they had come in through.
Ron shuddered involuntarily as a memory of his first, ill-fated, trip down here rose unbidden in his mind. Shaking the thoughts out of his head, he fished around in the pocket of his robes for the fist-sized globe of the key. "This place still gives me the creeps." He muttered, momentarily forgetting who he was with.
"Afraid, are we?" The strain of carrying the deceptively small and dangerous box was showing more and more, and Ron found himself momentarily transfixed by a large vein that had popped out in Malfoy's forehead and which was now throbbing hypnotically.
"No!" He exclaimed incredulously, tearing his eyes away as he held the globe out in front of him and concentrated, squeezing to activate it. After a few seconds a wave of magic the same pearly shade of pink as the key itself pulsed out from the key, splashing against the walls and leaving identical horizontal lines on each of the doors.
Ron lowered his hand and watched as the colour began to wash out from these lines, covering the doors for a second before fading to leave only a few words on each of the doors. Glancing around, he identified the one marked 'Exit' and let out a sigh of relief.
"Which one?" He barked at Malfoy, impatient to get out of there.
"The Prophecy Room." This time Ron found himself transfixed by the size which Malfoy's nostrils had stretched to as Malfoy struggled to keep hold of the dangerous, and apparently heavy, box. "What?" Malfoy snapped when he caught him staring.
"I've never seen a vein that big..." There was a note of awe in his voice. "And your nostrils..."
"Oh shut up and get on with it!"
Ron snorted as Malfoy took a deep breath, obviously trying to calm his tense nerves.
"Why don't you just levitate it?"
"What kind of idiot are you? Cast a levitation charm on an unknown Dark artefact which has already nearly killed people just for casting revealing charms on it?"
"Oh, right. I s'pose not." Turning away to hide his embarrassment, he found the door that was marked
Time Room
Leading to
Prophecy Room
and pushed his way through the door, pausing to hold it open for Malfoy as he slipped the key back into his pocket.
Part of him wondered what they could possibly have found that could have need to be stored in the Prophecy room but that stray thought was soon quashed by the rational part of him which loudly cut in going 'la, la, la, I can't hear you'. Questions like that led to worrying discoveries and the conscience-prompted need to do the right thing and that was most definitely Harry's forte. Ron just usually ended up along for the ride.
"C'mon will you?" He tried to chivvy Malfoy along, getting impatient at the length of time that it was taking him to do something as simple as walk through the door.
"Piss off! This thing's bloody heavy!"
"Serves you right for being evil." Even as he said it, he knew that his retort was childish to the millionth degree but he couldn't help it; there was just something about Malfoy which brought out that part of him which would always remain about 14 years old.
"Serves you right for being evil!" Malfoy mimicked in a falsetto voice, pulling what could only be described as a weasely face. "One day maybe you'll have an original thought." It appeared that he was not the only one afflicted by an over-active inner child.
He thought, for a moment, about hitting Malfoy but instead settled for gesturing behind Malfoy's back as he struggled to regain his grip on the box, grunting as he hoisted it up.
Slouching around him, Ron took his time as he made his way towards the other door, absently glancing around at the clocks, time turners, and other un-identifiable items that filled the room.
Ron only realised how much strain Malfoy was under when he heard his name being forced out between tightly clenched teeth and turning, found the blonde man grimacing as he teetered sideways, holding on to the box with only the very tips of his fingers.
Darting forwards, Ron tried to grab hold of the box, not registering either the terrified widening of Malfoy's eyes, nor his panicked cry of "No!". Before Ron had taken even one forward Malfoy had, in a moment of adrenaline-induced strength managed to dig his fingertips into the leather of the box and had swung it up, and away from him.
Without even thinking Ron lurched forwards and grasped at the box, trying to re-direct the object away from the glass bell-jar that it was colliding with. He was too late and he only managed to let out a despairing groan before he and Malfoy both crashed into the desk on which the bell-jar stood, the corner of the box catching on the edge, nearly jarring it out of their hands before they managed to get it securely onto the surface.
Looking up, he saw the painfully familiar source of the eerily glittering light and the small bird that continually cycled round on the current within the jar, changing from egg to bird and back again. He tore his eyes away from the circling bird and down to the box at which his and Malfoy's hands were both still clutching and which were already beginning to age, the knuckles swelling with the arthritis they had yet to suffer from.
All of this happened within seconds and before they had a chance to consider extricating themselves from the jar, just when he thought that things couldn't get any worse, he felt a sickeningly familiar pull just behind his navel.
"Oh boll-" the solidity of the floor was suddenly gone from under his feet but there was something more than the usual feeling of being transported by a portkey; his head seemed to be under immense amounts of pressure and he could almost feel his eyes popping like two ripe grapes, squeezed too hard. The next moment the forces had changed and he felt as though he was being pulled in opposite directions. These rapid changes of forces went on until he thought that he could stand it no longer, and then continued for longer until, finally, he felt beautiful, wonderful, solid ground underneath him again.


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Will this be the year of more broken resolutions, I think not as I have made none!
Yesterday I did some sewing for myself so hopefully I will have an entry for QuiltWest in May.
I also updated my website which I have been planning to do for a while. So far so good so now back to some to some sewing
Oh Angus
New Direction
I am continuing to work on updating my website and changing the theme. At the moment I have narrowed it down to 3 themes.
Another exciting step is that I am adding more workshops to my schedule which will be included in my 2017 program. I am working on class samples at the moment and enjoying every minute of it.
Besides updating this website and my workshops I am currently working on a Wendy Williams cushion. I have been working on this project for 2yrs since attending one of Wendy's pop up classes at the AQC in Melbourne, There is however light at the end of the tunnel as you can see I only have 5 flower heads to attach!
Weekend Sewing Project
I bought the pattern for these bags Sew Together Bags by 'Sew Demented' several months and it is only recently that I started making them. I completed the second one yesterday and I am fairly pleased with the result.
There are several little pockets on the inside for keeping sewing knick-knacks. This will be a very useful bag and make a lovely gift.
Revive The Blog
I can not believe that it over 18 months since I last posted. You might ask what I have been doing all this time. Quite a lot. Life has been busy with a sick husband and guild work over the last couple of years with very little time for my favourite hobby quilting. I have decided now is time to freshen up my website and try to blog daily. Let's see how it goes so watch out for changes in the coming weeks. And for the record I am slowly getting back into sewing photos will be posted very soon!
Bernina 820 using stitch 4
Recently I made a few journal covers and used many of the fancy stitches on my 820 for the quilting. Not all the stitches on the machine are suitable for quilting. I thought I would try to feature a stitch suitable for quilting with the dual feed every week on my blog. Not everyone is confident with free motion quilting so these stitches are can be used by those sewers who prefer to work with there walking foot.
Below are samples of variations of stitch 4 and how I stitched them. You can get your stitches to look like this by changing the stitch width and stitch length. These stitches are all 9mm wide.
Draw up either a horizontal or diagonal grid of your preferred size. I used a 1″ grid for the sake of demonstration but you can use a 2″ or 3″ grid etc on your quilt.
- Put the 20D open toe applique on and engage the dual feed.
- The top row of stitching is the default setting for stitch 4 so no changes have been made.
- The setting for the stitches on the horizontal grid are Width = 9mm and Length is 3.90
- This grid can be sewn either as parallel lines or cross hatched, both variations are shown on the Sample.
- Diagonal grid settings are:- Width 9mm and Length 3, you can see that by decreasing the stitch length the curves have become more wavy.
- This grid can also be stitched out as parallel lines or cross hatched. The choice is yours!
Missing my Bernina 820
I had hoped to do some quilting last weekend but managed to bend my sewing machine needle. This knocked the machine out of alignment resulting in skipped stitches as well as the needle threader not working. A service and repairs is going to be $115. As you can see I did a good job of the needle which is titanium!
I had wanted to play with the fancy stitches on the sewing machine as they have a lot of potential for quilting and could be useful for quilters who are not confident with free motion quilting. I recently used some of these stitches on journal covers I have been making and was really pleased with the results.
Instead I have had to content myself with drooling over the 820 stitch manual which I made last year (Can you believe I stitched all the stitches out). Nevertheless this did give me time to me select a few stitches that would be suitable for use instead of using a straight stitching or cross hatching when quilting.
Fun with Fillers
During the holiday I signed up for a Craftsy class with Leah Day as I really enjoy machine quilting and I am always ready to learn more.
Many of the fillers Leah teaches are very different to the type of fillers I have normally used on my quilts over the years. I have started work on my sample, but couldn't resist adding some of the fillers I have come to love.
I am using Silk Finish thread through the needle which is a Superior titanium 70/10 topstitch needle with bottom line in the bobbin. I work on the much maligned Bernina 820 which I have now grown to love. I must admit I thought I had made a big mistake when I first got the machine 5 years ago and could quite easily have thrown it out the window! Good job it is a heavy machine. It has been a matter of coming to terms with a different way of operating a sewing machine. I now love using a wide variety of various threads on this machine and look forward to the challenge of using different threads on it. I am amazed at some of the machine's capabilities for quilts with using fancy stitches instead of straight stitch only and will show some of the possibilities in a later post.
Having Fun!
Happy New Year everyone! I hope this year brings you all health and happiness and plenty of opportunities to sew. Have you made any New Year resolutions? I have only made one and that is to find more time to sew. Our guild activities have kept me so busy during the last couple years so I intend to make time to do what I enjoy most… machine quilting. At the moment I am having way too much fun, working on a few samples for my workshop in March. My friend Phil Thomas has let me use some of the beautiful fabrics she bought in Bali and they are a delight to work on. I am experimenting and playing around with different thread combinations to see what suits my machine best. On this small sample I am working with I have used Mettler Polysheen and Aurifil cotton 50wt thread with a size 70/10 Superior Titanium needle. The quilt is reversible and I am working on the burgundy side, but must say I prefer the back of the quilt at this stage though I could change my mind once the quilt is completed! The images above are of the front of the quilt. Below is the reverse of the little quilt. As you can see it is a work in progress which I hope to complete by the end of the week!
An Update Finally
Wow it is nearly 2 years since I last posted. What have I been doing in time you might ask, unfortunately not a lot of sewing. DH recently got me a new puppy Angus, he is really cute, but being a Jack Russell is full on. I have however managed to get to my sewing machine out in the last couple of weeks and completed a place mat for WAQA's community quilts. These will be distributed around Christmas time!
And now here's Angus! he came from Bathhurst NSW!