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Anna Bligh marks three years since she was sworn in as Qld Premier today....
ABC Tropical Queensland Archives
Stories from 13 September, 2010 on ABC Tropical Queensland (13 items)
Authorities in north Queensland are set to reassess the search for a man who fell overboard from a fishing trawler off Mackay....
The Isolated Children's Parents' Association (ICPA) says the Queensland Government seems to have had a change of heart about the future of small ...
More than 4,500 volunteers across north Queensland took part in the weekend's Great Northern Clean-Up....
Queensland Health (QH) authorities are checking another two possible cases of measles in the state's north....
Recreational fisherman Ron 'Mengie' Meng has had a great start to the week reeling in a 34 kilogram Spanish mackerel. ...
Several local sporting competitions played out their respective grand finals over the weekend and the Mackay Cutters played their final game in the ...
A union representing health professionals says some of its members want to strike over the pay dispute with the Queensland Government. The Liquor, ...
Authorities are set to reassess the search for a man who fell overboard from a fishing trawler off Mackay....
Anna Bligh marks three years since she was sworn in as Qld Premier today....
Member for Dalrymple Shane Knuth has written to the Queensland Government requesting a deadline extension for graziers completing environmental ...
Authorities in north Qld will focus on searching islands for a missing Irish tourist who fell off a trawler....
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Shop Birmingham, AL
Baby Equipment Rentals - Birmingham, AL Rent daily, weekly or monthly.
Crib Rental ~ Car Seat Rental ~ Highchair Rental ~ Stroller Rental ~ Baby Bath Rental ~
Baby & Toddler Toy Rental ~ Exersaucer Rental ~ Baby Gear Rental
Rockabye offers baby gear rental with either Delivery or Airport Car Rental Facility Drop Off/Pickup in Birmingham, Alabama. Delivery fees are $30 and up based on distance as determined by Zip Code. A Rush Fee of $20 applies to all orders beginning less than 48-hours from time of confirmation. Every order is subject to a $40 rental minimum and applicable taxes and delivery.
Our self pickup location is in Hoover and orders are made available by appointment only. This is NOT a store to shop, please do not arrive without a confirmed order and appointment. Our Self Pickup/Self Return location is at the Extra Space Storage Management Office, 3050 Bowling Drive, Hoover, AL 35242. Our representative will call you a day or two in advance of your order to schedule a time for you to pick up the equipment.
Initial prices shown are for rental up to 2 nights, Select the item and change the Rental Period to see rate for your rental length.
All equipment is meticulously cleaned and safety inspected. All recalled items are removed from inventory. Our rental cribs all adhere to the new Federal Safety Standards, drop-side cribs are not available. Rockabye provides equipment on a first come/first served basis. Customers are encouraged to place orders early around peak rental periods and holidays. By placing a confirmed order with Rockabye Baby Rentals, you accept our complete terms and conditions and agree to return equipment with all accessories undamaged and as indicated on the delivery order.
IMPORTANT - Before confirming an order, please review our Terms and Conditions! Providing payment is acceptance of our Terms and Conditions in full. A complete copy of our Terms can be found on our shopping site at http://shop.rockabyebabyrentals.com/termsandconditions.sc or on our informational site http://www.rockabyebabyrentals.com/Terms.htm

subset: commoncrawl


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The energy of the music we play onstage is absorbed by the audience members, who carry it away with them. We performers carry it also. So I play music that expresses love, joy, gratitude, and the Divine as beautifully as I can in the hope that we will all benefit.
I play guitar and bass for Tao Theory, The Convergents, Mukti, Ananda Rasa Kirtan, Radiance Kirtan, Mirabai Warkulwiz, Acharya Mangalananda, and Musica Divina. I've also recently performed with Jai Uttal, Prema Hara, Prajna Vieira, Daniel Paul, Ben Leinbach, Prabhu Nam Kaur, and Gregg Filler.
I also compose and perform as a solo guitarist. I hold a Master of Music degree from Florida State University. I perform original music, classical guitar repertoire, and a few jazz standards for private events and public venues.
This year I've performed over 100 times. In the past few years I've performed at Bhakti Fest West, Shakti Fest, Mt. Shasta Yoga Fest, Grace Cathedral San Francisco, M. A. Center San Ramon, San Geronimo Valley Community Center, Open Secret Bookstore San Rafael, and at many yoga studios and private events around the Bay Area.
For current performance info and other interesting bits, please see my Facebook page.
Please enjoy these solo guitar pieces that I wrote and recorded recently.
Contact me directly:
- Greg Barnett
- firstname.lastname@example.com
- 415-810-5005


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|Message view
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|Chris Hostetter <hossman_luc...@fucit.org>
|Re: To Sort or not to Sort
|Fri, 17 Dec 2004 01:31:26 GMT
: In my application, users search for messages with Lucene. Typically, : they are more interested in seeing their hits in date-order than in : relevance-order. In reading my ebook copy of "Lucene in action" (wish : I'd had that a year ago), I find that one of the features added in 1.4 : was the ability to ask for hits in an order based on a field. It also : looks like adding the field necessary to get things by date order is : straight forward. When considering issues like this, it's important to consider what is really important to your users: Do they eally want to see items strictly ordered by date, or do they want to see results sorted by relevancy -- where the "recentness" of an item influences how "relevent" it is. For example, when I search theLucene users mailing list for "RangeQuery" I want more recent messages to appear first, but I'd still prefer that a slightly older message "bubble up" in the list if the Subject includes "RangeQuery" and the body mentions RangeQuery dozens of times -- because it's likely to be very relevent then more recent messages which only mention RangeQuery once or twice -- but I don't want results that are strictly sorted by term frequency, becuase then messages from 3 years ago (and several Lucene revs ago) might be at the top of the list. Depending on how you maintain your index, there are a couple of different ways of achieving a goal like this. if you rebuild regularly, then just giving your more recent documents a higher boost is one way to go. another would be to use several FilteredQuery(RangeFilter) with several increasing intervals of dates (ie: today OR the past week OR the past month OR the past year) so that more recent documents match all of the clauses, and older documents match fewer (or none) -Hoss --------------------------------------------------------------------- To unsubscribe, e-mail: email@example.com For additional commands, e-mail: email@example.com
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The revised Markets in Financial Instruments Directive (MiFID II) and the accompanying Markets in Financial Instruments Regulation (MiFIR) regulate the provision of investment services in respect of a multitude of financial instruments traded on regulated trading venues as well as OTC Markets.
The new legal framework entered into force on 1 July 2014. Various provisions must be transposed into national law by July 2016, while the detail regarding others is to be further specified in additional rules developed by the European Securities and Markets Authority. Application is planned for January 2018.
The impact of the MiFID II / MiFIR rules is far-reaching and will have a significant impact on the EU's financial markets. These include market structure changes; transparency requirements for a broader range of asset classes; the obligation to trade certain derivatives on-exchange; requirements on algorithmic and high-frequency-trading and new requirements for commodity derivatives.
CME Group is currently evaluating the impact of the MiFID II/MiFIR reforms on our European infrastructure.
For more information about this regulatory reform:


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Peter A. Matteo, 86, of Dover, died after a short illness on Wednesday, Sept. 5, 2001, at the home of his daughter in Severna Park, Md.
Mr. Matteo was born in Dover and lived there all of his life.
He was a furniture salesman at Furniture Liquidators in Rockaway for 20 years. He was also an avid bowler, enjoying the sport since 1940.
Mr. Matteo was predeceased by his wife, Marcella, who died in 1964. Mr. Matteo is survived by a son, Peter G. of Dover; a daughter, Michele Jarry of Severna Park, Md.; two grandsons, Matthew Jarry and Nicholas Jarry; a brother, John of Florida; his long-time companion, Alma Sickles; and many nieces and nephews.
Donations may be made to Hospice of the Chesapeake, Millersville, Md., 21108, or to the Harry and Jeanette Weinberg Oncology Center of Johns Hopkins Medical Center, Baltimore, Md., 21231.
Arrangements were by Tuttle Funeral Home, 272 Route 10, Randolph Township.


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Real estate is more than land and buildings. It is an investment. It may be the family home. Paul has over 20 years of experience with real estate transactions, purchase and sale issues, construction contracts and defects, mechanics liens, payment/collection problems, landlord tenant matters, lot line disputes, easements, and issues with government agencies involving residential and commercial real estate matters. Paul represents buyers, sellers, landlords, tenants and contractors in real estate transactions and disputes. Paul is also a skilled negotiator and can facilitate a transaction as an advocate or as a neutral mediator. Contact Paul for an initial consultation. Read the depositions of real estate agents by Paul: http://realestatedeception.com/
Mechanic's lien Commercial real estate Landlord or tenant Real estate Construction law Family law
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Gajar Ka Halwa
Brief Description
Gajar Ka Halwa is commonly known as gajrala. Gajar Ka Halwa is a traditional Punjabi dessert. Gajar Ka Halwa preparation requires carrots and milk as its main ingredients. Gajar Halwa recipe can be eaten in any ways either warm or cold. Since Gajar Ka Halwa Preparation can work well either with dry fruits or not. It totally depends upon ones taste.
|Preparation Time
|15 mins
|Cooking Time
|30 mins
Ingredients for Fruit Kheer
- Carrots-1 kg
- Milk-1 litre
- Cardamom Seeds -1 teaspoon
- Water -3/4 cup
- Ghee-3 tablespoons
- Raisins-1 to 2 tablespoons
- Almonds-1 to 2 tablespoons
- Pistachios-1 to 2 tablespoons
- Sugar –half kg
How to make Gajar Ka Halwa
- Grate the carrots.
- Put some raisins in water for 30 to 35 minutes.
- Now chop the nuts.
- Put the water to boil, as soon as it starts boiling add the carrots to it. Cook for about 10 minutes.
- Now add milk to the carrots. Cook it for about an hour while stirring occasionally.
- Now add sugar to the mixture and cook it well till the whole milk get disappeared.
- Then, add ghee, crushed cardamoms and the raisins. Mix well.
- Gajar ka halwa is ready to be served. Serve it with garnishing of almonds and pistachios.
Gajar Ka Halwa
Some Useful Tips
You can preserve the Gajar ka halwa in fridge for a week or in freezer for about couple of months.
Other Similar Recipes
- Parwal ki burfi
- Dry fruit barfi
- Gulab jamun
- Kaju pista roll
- Kala jamun


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Posted: Wednesday, May 10, 2017 11:09 AM
New Glock 43 9mm Semi-Automatic Pistol, 6 round magazine. Additional magazine plus loader and cleaning brush included. This is the perfect carry pistol! The grip has a built in beaver tail design for a high and tight grip. Large magazine catch for easy removal. Two magazines, cleaning brush, magazine loader, and hard case included.
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Play it safe. Avoid Scammers.
Most of the time, transactions outside of your local area involving money orders, cashier checks, wire transfers or shipping (especially overseas shipping) are scams or frauds.
Report all scam attempts to firstname.lastname@example.com.


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United, please take back Januzaj. He is utter dog shite.
Mourinho has now lost 16 of his past 34 matches (after losing 16 of the previous 111) and lost three in a row for the first time in ten years. Has the magic gone? It's still very early but I think you have to be a little bit concerned about the apparent lack of plans, he already just seems to be throwing darts and hoping that he'll fluke a bullseye.
United, please take back Januzaj. He is utter dog shite.
Yeah, again I stress, United take back Januzaj.
1 point after 6 games? Jesus today's game sucked huge donkey dick, like badly. How the fuck can you give away a 2-goal lead? Do we blame Moyes, that's the easy thing to do but the player's can't get off free either.
We're going down this season, being made more painful when the mag bastards pass us on the way up.
They had an exceptional season that also saw Chelsea and Manchester City shit the bed. Arsenal didn't have the best year last season and still managed to finish 2nd. Last season was an anomaly.
They also lost Kante who was a big part of their attack last season. They'll be lucky if they challenge for Champions League again this year.
The most worrying thing for Leicester in my view is just how porous their defence has been. It was always unlikely that Mahrez and Vardy would replicate their prolific streak, but given there were no changes in the back four I expected them to hold fairly tight. Goals from corners like they were conceding against United is just one thing you wouldn't be accounting for given their strength with Huth and Morgan. I think Ranieri really has to go to a three man midfield and solidify things - the 4-4-2 is nice and was such a big success last season but without Kante they are proving to be far too exposed, conceding four goals in three straight games.
Looking at them as the "best team in England" is an anomaly as last season was so unbelievable, and only a handful of their players have played in European competition before. From any perspective they are doing fantastically well.
- Kirchoff
- Januzaj
- Borini
- Cattermole
- Anichebe
- Pickford
- Pienaar
- Rodwell (well, he is a walking sick note)
Our current injured list is sublimely banterific.
Fairly astonishing results from last night, underlining just how poor the quality of defending in the league is these days. 29 goals in 6 games. Obviously the most noteworthy was Leicester turning over Man City, something which I don't think anyone would have seen coming given Leicester's form as of late - but they took advantage of City's abject defending and truly awful tactical structure. Guardiola had an absolute nightmare, compounding his side's loss of strength through suspension by trying to force square pegs in to round holes - it seems like all the changes he's made (more than any other manager in the league this season) has completely confused most of his players who have no idea what they are supposed to be doing.
Just because there are a few managers who have found some success with a back-3 doesn't mean it is a magic bullet, and it certainly isn't when you have players at your disposal like Stones, Kolarov and Sagna. I think they'll have to try and invest (once again) in the January transfer window to try and shore up the backline. Kompany is simply too unreliable, Stones needs someone with more experience and strength alongside him, Otamendi is too impulsive, and the aforementioned Kolarov and Sagna are just not central defenders.
So Wayne Rooney to China, eh? Thoughts?
Nothing official yet but it's looking like it could happen. I think two things:
1) Rooney should take it and get his pay day. He could play for a year and be set for life (not that he isn't already but you know what I mean $$$)
2) Rooney doesn't owe the fans anything. Why stay at Old Trafford and watch his career dwindle to nothing from the bench?
Just my two cents.
Froggy and/or Tenks still lurk around here right?
Leicester City have tonight SACKED title winning manager Claudio Ranieri.
Moments like this is why I don't really watch football anymore. It's a heartless, soulless game
( ?_?) - 97% of PW suddenly think Michael Cole is awesome. If you're part of the 3% that still thinks he is an annoying tool, copy & paste this into your sig.
Currently Playing: Suikoden 1, Max Payne 1, Beyond Good and Evil, MGS Peace Walker
Mourinho was sacked
Pelligrini was replace
Ferguson retired
Mancini was sacked
Anchotti was sacked
Football managment, such a funny business.
It's obviously sad but I think it was a move that they had to make, Leicester haven't just been bad recently, they've been absolutely diabolical, and with others around them like Swansea and Hull improving under their new managers, are now more than a legitimate candidate for relegation.
It would have been nice from a sentimental perspective for him to have at least got see the Champions League campaign through, as they're still vaguely in with a chance in the second leg against Sevilla, but that home league game against Hull before hand is a massive fixture, and then there's back to back home games against Stoke and Sunderland which they really need to get some points from, so you can understand the rationale of making a change now having not scored in the league this calendar year.
Yeah, they'll beat us
Arsenal just got whooped 10-2 on aggregate over Bayern Munich.
Fans are calling for Arsene Wenger to be fired. You think this may happen?
Still My Champ
Thanks, surrender.
Nah. They'll give him an extension and he'll retire a Gunner in a few years.
If the team isn't already in "rebuild and scout Arsene's replacement," mode, they will be come summer. They probably won't have a choice.
Alexis is gone (he was laughing on the bench after Bayern's 5th), Cazorla is gone, not sure what Ozil will want/do because I don't know his contract details (he seems indifferent at best though), Cech is old...yeah, time to get serious about filling the obvious gaps and focusing on being a contender in a few years and not next year.
There are currently 1 users browsing this thread. (0 members and 1 guests)


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The property makes the perfect place in Ao Nang to get away from the stresses of life, where guest will discover the real serene in their individual lake side bungalows featuring modern style or in the romantic bamboo huts.
The hotel is conveniently located only 700 metres away from Ao Nang Beach and Nopparat Thara Beach.
The bungalows are divisibly designed as detached house in the atmosphere of natural country style. Each guestroom comes with air conditioning and fan, its own private bathroom, hot shower, satellite TV, mini bar and refrigerator.
The resort offers restaurant and services guests both by land and by sea tourism for instance.
A car ride between Krabi International Airport and the hotel takes 40 minutes. The property is to be found located on the road to Ao Nang Beach, near the boxing stadium.
Shuttle bus service can be arraged at the hotel.
Room Facilities:
Satellite cable TV
Internet access
Mini fridge
Tea Coffee
Double bed
Individual airconditioned


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Church News
Christ Church seeks information on those buried in its cemetery
A walk through the Christ Church Parish Cemetery is not just a walk through the history of Middlesex County, but through Virginia and American history as well. Church records indicate that at least six graves were placed inside the footprint of the present building and a stone found during an earlier renovation has been placed in the aisle leading to the altar.
07.13.2011SRBA Annual Session convenes July 19-21
The 2011 Annual Session of the Southside Rappahannock Baptist Association will convene July 19-21 at First Baptist Church, Tappahannock, where Rev. Vernon Keeve serves as pastor. The theme for this session is "Ministering to People Where They Are" (I Corinthians 9:9-23).
07.13.2011Rev. Jesse Jackson to speak at Lebanon Baptist on July 31
On Sunday, July 31, Lebanon Baptist Church near Saluda will celebrate seven years of being in its new edifice on Route 17. "We have been blessed by God to have Rev. Jesse Jackson to speak for the occasion," said a church spokesperson.
07.13.2011Youth Day is July 20 at SRBA Convention
On Wednesday, July 20, at 9 a.m. the Southside Rappahannock Baptist Association (SRBA) will host Youth Day at the morning session of its annual convention, which will be held at First Baptist Church, Tappahannock, where Rev. Dr. Vernon Keeve is pastor.
More Church News: page 309 of 465 pages « First < 307 308 309 310 311 > Last »


text:
SiteLinkChecker checks Web sites for broken links and shows status of each link.
Use this security logo:
Freeware is computer software that is available for use at no cost or for an optional fee.
Tutorial not found. Let know us about any useful video tutorial.
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Updated Site Link Checker
Anyone may copy this programm for any purpose. You do NOT need any permission to copy this program to an archive, magazine,mailing list, FTP archive, etc... This programm may be used free of charge for any purpose including corporate and academic use. Any attempt to sell this programm must have the permission of the original author.


text:
Skip to Content
Home > Careers > MUHC Employment Process
View Additional Section Content
You will receive an e-mail notification once your application has been successfully submitted. Notification of important activity regarding your application, status, job posting notification matches, etc. will be sent to your email.
Note: If you are not receiving notification emails from University of Missouri Health Care Human Resources, we recommend checking your spam or junk folders (or other email filter) as emails may sometimes skip your inbox.
Once your application has been submitted, a representative from Human Resources will review your submission to determine if you meet the minimum required qualifications for the position.
Candidates who meet the minimum qualifications will then be reviewed and ranked based on their qualifications and work experience. The most qualified candidates will be asked to complete an online questionnaire as part of the selection process.
University of Missouri Health Care does NOT release the status of an application. In order to check your application's status, log on to your account and click the "My Jobpage" tab at the top of the page. You will be able to review the positions for which you have applied and the status.
Once the final candidate is identified, a member of Human Resources will contact the candidate to make a formal offer. Upon acceptance, the candidate will be required to complete a substance abuse test (including nicotine usage) and will be scheduled for a pre-employment health screen along with new hire paperwork.
For detailed information, please click here.
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text:
Define the Lowest Common Ancestor Problem. I.e. what does LCA(i,j) return?
How is a Euler tour different to a depth first search?
How long is the Euler tour of a preprocessed LCA tree?
Describe how to preprocess a tree ready for an LCA query.
Describe how to make a LCA query when solved using RMQs.
Without using the +/- RMQ algorithm. What is the prep time, space and query time complexity of the LCA solution using RMQs?
If the +/- RMQ problem can be solved with O(n) space, O(n) prep time and O(1) query time, what is the space, prep time and query time complexity for LCAs when solved using the +/- RMQ algorithm?
How can RMQs be solved in O(1) query time and O(n) space/prep time?
Created by msladey about 2 years ago


text:
Cities and counties won't be allowed to raise the minimum wage under a law signed Monday by Oklahoma Gov. Mary Fallin (R).
Under the new law, which passed the state legislature on party-line votes earlier this year, cities would be prohibited from raising the minimum wage above the $7.25 federal mark, and they would be prevented from requiring employers to provide sick days to their employees.
Backers of the new law said differing wages in different cities would be a hardship for businesses. In a statement, Fallin said different wage laws would hurt job creation.
In recent years, cities and localities across the country have unilaterally increased their minimum wages without waiting for state action. Santa Fe raised its minimum wage in 2003. Last year, voters in Seatac, Wash., a small suburb of Seattle, set the minimum wage for airport workers at $15 an hour. The Richmond, Calif., city council voted last month to raise the minimum wage to $12.30 an hour by 2017.
Los Angeles and Seattle are considering raising their minimum wages to $15.37 and $15 an hour, respectively.
But that option won't be available to Oklahoma municipalities. Supporters of a higher minimum wage are in the process of gathering the 80,000 signatures necessary to get an initiative on the ballot in Oklahoma City that would boost the minimum wage from the federal $7.25 an hour mark to $10.10 an hour.


text:
Beyonce reveals second baby plans!
Could there be another little Beyonce or Jay-Z in 2014?!
Openly gushing about daughter Blue Ivy, now 14 months, in the April issue of Shape magazine, Beyonce spoke of her future plans to expand her family with hubby Jay-Z.
In the interview the singer revealed that she "absolutely" wants more kids and will try for a second after her world tour.
"I'll probably start after this tour," she said. "We'll see if it happens." Her world tour kicks off on April 15 and ends on August 15.
Beyonce once told Vogue: "When I was younger, there were moments where I said, 'I'm not going to have children', and then moments when I wanted four, and now I definitely want another."
Images via I am Beyonce
"I just adore being a mother, hearing her say 'Mama' and call me when she needs something … It makes me feel like I have a real purpose here. I enjoy all the things people warned me would be tough to handle," she said in the interview.
"I'm most surprised by how much she's taught me," the proud mum added. "She's only a year old and I've learned so much about myself from her. Giving birth was one of my biggest fears, and having Blue forced me to face it. And now I recognise the strength I have. She teaches me to focus on the things that truly matter, like family, and to pay attention to each moment, because they go by so quickly."
I totally agree with Beyonce. Having children teaches you so much more about yourself. I honestly never knew how strong I could be in the face of fear, or how protective I could be. Mumma Bear can be tough at times! I also didn't realise how much I lacked patience until I had children. Sigh
Beyonce turns 32 this year so I guess the clock is slowly starting to tick away from her if she plans on having two or even three more children. Good luck to her I say!


text:
Response of human chondrocytes and mesenchymal stromal cells to a decellularized human dermis
Although progress has been made in the treatment of articular cartilage lesions, they are still a major challenge because current techniques do not provide satisfactory long-term outcomes. Tissue engineering and the use of functional biomaterials might be an alternative regenerative strategy and fulfill clinical needs. Decellularized extracellular matrices have generated interest as functional biologic scaffolds, but there are few studies on cartilage regeneration. The aim of this study was to evaluate
Normal human articular chondrocytes (NHAC-kn) and human mesenchymal stromal cells (hMSC) from healthy donors were seeded in polystyrene wells as controls (CTR), and on decellularized human dermis batches (HDM_derm) for 7 and 14 days. Cellular proliferation and differentiation, and anabolic and catabolic synthetic activity were quantified at each experimental time. Histology and scanning electron microscopy were used to evaluate morphology and ultrastructure.
Both cell cultures had a similar proliferation rate that increased significantly (
The results obtained showed that in
Articular cartilage lesions following acute trauma or pathological conditions such as osteonecrosis, osteochondritis and rheumatoid arthritis are responsible for the highest rate of disability worldwide
Xenogenic and allogenic decellularized extracellular matrices (ECMs) have aroused great interest as functional biologic scaffolds also in cartilage tissue regeration
To the authors' knowledge, there are few studies that assess the biological properties of acellular ECM membranes as scaffolds for cartilage tissue engineering and regenerative medicine, not only in terms of proliferation and viability, but also anabolic and catabolic synthetic activity
The aim of the present study was to evaluate
Decellularization of human dermis
Decellularized human dermis (HDM_derm) was taken from the back of four multi-organ and/or multi-tissue donors by following strictly Directive 2004/23/EC and Italian Transplant Center Guidelines on harvesting, processing and distributing tissues for transplantation.
In sterile conditions, using an electric dermatome, the epidermis was separated from the dermis trunk area and dermis samples of about 10 cm2 were dissected. The thickness of the dermal samples was about 0.8 mm. The dermis grafts were rinsed with 0.9% NaCl solution and stored in this solution for transport (2-4°C) to the treatment station (< 12 hrs) where they were submitted aseptically to a combined treatment of decellularization. The tissue was treated with 2.5% trypsin (GIBCO, catalogue n. 15090) and placed in an incubator overnight (5% CO2 at 37°C), washed twice with sterile 0.9% NaCl to remove trypsin remnants, then dipped in RPMI medium (containing 10000 IU/ml penicillin, 10 mg/ml streptomycin, 25 μg/ml amphotericin B) for 15 minutes and sealed inside cryofreezing bags (Hemofreeze bag – DF 700–3 by Fresenius Kabi) without adding cryoprotectors. Finally, the dermis was submitted to irradiation with γ rays (100 Gy) and stored in nitrogen vapour at -180/-190°C. This method provides a decellularized ECM scaffold, as shown by histology (H&E, DAPI stain), Transmission Electron Microscopy, cell viability test and DNA amount (Picogreen DNA Assay)
Cell proliferation and biosynthesis on decellularized human dermis
Normal human articular chondrocytes (NHAC-kn, Clonetics™ Cell System, BioWhittaker Italia, Caravaggio, Italy), derived from a single-donor knee articular cartilage, and human mesenchymal stromal cells (Poietics™ hMSC, Lonza Walkersville Inc., Walkersville, USA) harvested and cultured from bone marrow of a single donor, were used for the experiment. NHAC-kn and hMSC cells were expanded using the Chondrocyte Growth Medium (CGM, containing fetal bovine serum 5%, gentamicin sulphate-amphotericin B 0.1%, b-Fibroblast Growth Factor 0.5%, R3-Insulin-like Growth Factor-1 0.2%, insulin 0.2%, transferrin 0.1%) and the Mesenchymal Stem Cell Growth Medium (MSCGM, containing 10% FCS, 30 μg/ml Gentamicin and 15 ng/ml Amphotericin, Lonza Walkersville Inc., Walkersville, USA). Four different batches of HDM_derm were aseptically cut into 10 × 10 mm2 pieces; NHAC-kn, at passage 1, or hMSC were seeded onto forty-eight specimens at a density of 1 × 105 cells. The same concentration of NAHC-kn and hMSC cells was seeded in empty polystyrene wells as control (CTR). After 24 h incubation, of growth media were changed with Chondrocyte Differentiation Medium (CDM, containing fetal bovine serum 5%, gentamycin sulphate-amphotericin B 0.1%, Transforming Growth Factor β-3 0.5%, R3- Insulin-like Growth Factor-1 0.2%, insulin 0.2%, transferrin 0.2%, and ascorbic acid 2.5%) in NHAC-kn cultures and with Complete Chondrocyte Induction Medium (Lonza Walkersville Inc., Walkersville, USA) in hMSC cultures. After 24 h of incubation, the medium was collected and non-adherent cells were counted. Cell adhesion was calculated as the difference between seeded cells and non-adherent cells, present in the medium and attached to the 24-well plate bottom, expressed as a percentage of the number of the seeded cells. At 7 and 14 days, the supernatant was collected and the following tests were performed: procollagen type II C-propeptide (CPII ELISA, IBEX Technologies Inc., Montreal, Quebec, Canada) and aggrecan (Human Aggrecan EASIA™ ELISA kit, BioSource Europe SA, Nivelles, Belgium) for phenotype expression, the multifunctional peptide transforming growth factor- β1 (TGF-β1, quantikine human TGF-β1 immunoassay, R&D Systems, MN, USA) for anabolic growth factor synthetic activity, interleukin1β (IL-1β, quantikine human IL-1β immunoassay, R&D Systems, MN, USA) and matrix metalloprotease 3 (MMP3, quantikine human MMP3 immunoassay, R&D Systems, MN, USA) for inflammatory and catabolic response assessment, respectively. The measured protein concentrations were normalized by Total Protein concentration (Total Protein Kit, Micro Lowry method, Petterson's Modification, SIGMA®, Missouri, USA). The cell proliferation reagent WST-1 (Roche Diagnostic GmbH, Penzberg, Germany) test was performed and quantified spectrophotometrically at 450 nm with the reference wavelength at 625 nm. Results were expressed as % Vitality calculated as follows:
Histology and scanning electron microscopy
After 14 days of cell culture, the seeded membranes were studied to evaluate chondrocyte and mesenchymal stromal cell colonization inside HDM_derm by histology and scanning electron microscopy (SEM). For histology, specimens were fixed in 10% buffered formalin solution in PBS (pH 7.4), embedded in paraffin and sections were cut to 6-μm thickness and stained with Haematoxilin & Eosin, Safranin O and Alcian Blue. Images were grabbed by using an Olympus BX51 microscope equipped with XC30 Olympus camera. For SEM, specimens were fixed in 2.5% v/v glutaraldehyde (pH 7.4 in PBS 0.01M, 1h) and dehydrated in graded ethanol series. After a passage in hexamethyldisilazane, the samples were air dried, then sputter-coated with gold before being examined by a Philips XL-20 SEM.
Statistical analysis
Statistical evaluation of data was performed using the SPSS/PC+ Statistics TM 12.1 (SPSS Inc., Chicago, IL USA) software package. Data are reported as Mean ± SD at a significance level of
At 24 hours after seeding, cells adhered consistently to HDM_derm membranes (93% and 98% for NHAC-kn and hMSC, respectively). At 7 days, the cell vitality index was 98% for both cell cultures seeded on HDM_derm. After 14 days of culture, these indexes significantly (
Significant phenotypic marker expression data obtained from NHAC-kn and hMSC cultures seeded on HDM_derm were normalized to those of their controls (Table
Histology and Scanning Electron Microscopy (SEM)
The main histo-architecture of the acellular extracellular matrix was retained. After 14 days in static culture, both cells grew prevalently on the HDM_derm surface. NHAC showed rounded features forming aggregates along the HDM_derm surface and penetrating in the deeper layers of the decellularized membrane (Figure
Figure 1
Histology and SEM patterns of NHAC-kn and hMSC seeded on HDM_derm at 14 days
Histology and SEM patterns of NHAC-kn and hMSC seeded on HDM_derm at 14 days. Histological and SEM images of NHAC-kn (a,c,e,g) and hMSC (b,d,f,h). The NHAC-kn and hMSC cells are qualitatively recognizable in HDM_derm. Haematoxilin & Eosin staining (a,b), magnification of 40x (scale bar = 20 μm): both cell types grew superficially and deeply colonized the HDM_derm. Safranin O staining (c,d), magnification of 80x (scale bar = 10 μm): presence of proteoglycan synthesis (dark red), predominantly inside cells and equally present in both primary cells. Alcian blue staining (e,f), magnification of 80x (scale bar = 10 μm): around both primary cells light blue stain indicated the presence of GAGs deposited on the decellularized matrix. SEM images show a complete colonization of HDM_derm scaffold by NHAC-kn with collagen network still visible; NHAC-kn retained their viable morphology and cell-to-cell relationship (g). Sparse distribution of the few hMSC cell aggregates is present (h).
SEM analysis performed 14 days after seeding on HDM showed different patterns for NHAC-kn and hMSC, respectively. A homogeneous and multilayered surface-covering colonization with possibly living cells was found for NHAC-kn on HDM_derm: they had a polygonal-rounded morphology or fusiform shape. Most of the cells were connected to each other by forming cell aggregations and were tightly interwoven within the collagen network. Conversely, hMSCs partially covered HDM_derm by cell aggregates associated with a clear-cut collagen meshwork (Figure
The aim of the present study was to assess the influence of HDM_derm on two human primary cells – NHAC-kn and hMSC −, in terms of CPII, aggrecan, TGF-β1, IL-1β and MMP-3 release, in order to evaluate its possible use as a scaffold for
The current results showed that HDM_derm supported NHAC-kn and hMSC cell adhesion, vitality, chondrogenic differentiation and synthetic activity. The NHAC-kn cultures maintained their phenotype with significantly increased synthesis of CPII and aggrecan, whereas the hMSC cultures showed a significant increase in aggrecan and TGF-β1 secretion. Furthermore, HDM-derm did not induce the release of IL-1β and MMP-3 that are often used as markers to measure inflammatory and catabolic stimuli
The reason why HDM_derm retained bioactivity upon decellularization might be because it participates and directly enhances cellular adhesion and proliferation indexes as found with NHAC-kn and hMSC, at least in the early stages of culture. Some authors found differences among growth factors (GFs) such as VEGF, FGF and TGF-β release in the extraction vehicle from different decellularized membranes
Histological and SEM investigations supported
The experimental set up of the study had some weaknesses. First of all, the lack of a more clinically relevant matrix as a control was a major limitation. The ideal control material for the present study would be a dermis from the same donors decellularized by an already developed and recognized procedure. Unfortunately, these decellularization techniques that are used in the production of clinically accepted products are patented and it was not possible to reproduce this technique on the dermis derived from the donors included in the present study. Therefore, it was preferred to perform experiments without using other materials with different chemical and physical properties such as synthetic scaffolds or hydrogels.
The use of single-cell seeding density and relatively short culture time was the second weaknesses of the current experimental set up. Therefore, the seeding of HDM_derm probably needed an improvement in the
A further improvement in the current study set up might be to adopt a dynamic standardized culture condition by using bioreactors able to ensure efficient cell seeding, colonizing the scaffold deeply and also able to reproduce a mechanical stimulus to improve the cartilage-construct differentiation and maturation as well as regulate microenvironmental key factors in promoting chondrogenesis, such as oxygen concentration and pressure, or the presence of proteases
Finally, the strengths of the current study were: (1) the use of a new decellularization method on allogenic human dermis that provided a reliable 3D structural and biological scaffold for chondrocytes and MSCs; (2) the use of primary non-transformed human cells that closely simulate a living model, thus yielding more physiologically significant results than animal derived cells.
Over the last few years, decellularized xenogenic and allogenic ECMs have started to be investigated for cartilage tissue engineering, because they retain structural and functional proteins and antibacterial activity, which has been shown within degradation products of biological scaffolds composed of extracellular matrix
In summary, to the present authors' knowledge, this study is the first piece of evidence to support the potential use of human decellularized dermal matrix as a scaffold for cartilage regeneration. The results showed that the developed HDM_derm might be used as a scaffold by acting as a biological and bioactive substrate for cell seeding, proliferation and synthetic activity. HDM_derm sustained the growth and colonization of a well-differentiated phenotype NHAC-kn as well as undifferentiated hMSCs. These results support the use of HDM_derm for further preclinical
CDM: Chondrocyte Differentiation Medium; CGM: Chondrocyte Growth Medium; CPII: Procollagen Type II C-Propetide; CTR: Controls; ECM: Extracellular Matrix; FGF: Fibroblast Growth Factor; GF: Growth Factor; HDM_derm: Decellularized Human Dermis; hMSC: Human Mesenchymal Bone Marrow-Derived Stromal Cells; IL-1β: Interleukin 1 Beta; MMP-3: Matrix Metalloprotease 3; MSCGM: Mesenchymal Stem Cell Growth Medium; NHAC-kn: Normal Human Knee Cartilage-Derived Articular Chondrocytes; SEM: Scanning Electron Microscopy; TGF-β1: Transforming Growth Factor Beta1; VEGF: Vascular Endothelial Growth Factor.
Competing interests
The Authors EB, DM, RG, MF patented the decellularization method.
Authors' contributions
Contributions of the authors to the manuscript included:
The present work was supported by Ministero dell'Università e della Ricerca, FIRB 2010 Project n. RBAP10MLK7 "Scaffolds for skeletal tissue regeneration: preclinical evaluation of biocompatibility and effectiveness" and by the Project "Regione Emilia Romagna Programma di Ricerca Regione-Università 2007-2009" (Regenerative Medicine in Osteoarticular Disease). The authors wish to thank Mr. Keith Smith for his assistance in language supervision.
Strategies for articular cartilage lesion repair and functional restoration
The inductive effect of bone morphogenetic protein-4 on chondral-lineage differentiation and in situ cartilage repair
Functional biomaterials for cartilage regeneration
Mechanical properties of natural cartilage and tissue-engineered constructs
Biologic scaffold composed of skeletal muscle extracellular matrix
Development and evaluation of a decellularized membrane from human dermis
Human dermal matrix scaffold augmentation for large and massive rotator cuff repairs: preliminary clinical and MRI results at 1-year follow-up
Decellularized extracellular matrix derived from porcine adipose tissue as a xenogeneic biomaterial for tissue engineering
A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells
Fabrication and repair of cartilage defects with a novel acellular cartilage matrix scaffold
A sandwich model for engineering cartilage with acellular cartilage sheets and chondrocytes
Recellularization of decellularized human adipose-tissue-derived extracellular matrix sheets with other human cell types
Decellularized cartilage matrix as a novel biomatrix for cartilage tissue-engineering applications
Decellularized human dermis to treat massive rotator cuff tears: in vitro evaluations
Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis
The role of growth factors in cartilage repair
A review of decellularized stem cell matrix: a novel cell expansion system for cartilage tissue engineering
The retention of extracellular matrix proteins and angiogenic and mitogenic cytokines in a decellularized porcine dermis
Preserved extracellular matrix components and retained biological activity in decellularized porcine mesothelium
Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering
Chondrogenesis and cartilage tissue engineering: the longer road to technology development
An in vitro study of collagen hydrogel to induce the chondrogenic differentiation of mesenchymal stem cells
Chitosan scaffolds for osteochondral tissue regeneration
Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in self-gelling alginate discs reveals novel chondrogenic signature gene clusters
Effect of chondroitin sulphate C on the in vitro and in vivo chondrogenesis of mesenchymal stem cells in crosslinked type II collagen scaffolds
Hyaluronic acid-binding scaffold for articular cartilage repair
Simultaneous regeneration of articular cartilage and subchondral bone in vivo using MSCs induced by a spatially controlled gene delivery system in bilayered integrated scaffolds
Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends
Fibrin glues in combination with mesenchymal stem cells to develop a tissue-engineered cartilage substitute
Cultured cell-derived extracellular matrix scaffolds for tissue engineering
Culture of human bone marrow-derived mesenchymal stem cells on of poly(L: -lactic acid) scaffolds: potential application for the tissue engineering of cartilage
Chondrogenic differentiation of human adipose-derived stem cells in polyglycolic acid mesh scaffolds under dynamic culture conditions
TGF-β3 immobilized PLGA-gelatin/chondroitin sulfate/hyaluronic acid hybrid scaffold for cartilage regeneration
A study on repair of porcine articular cartilage defects with tissue-engineered cartilage constructed in vivo by composite scaffold materials
Self-assembly-peptide hydrogels as tissue-engineering scaffolds for three-dimensional culture of chondrocytes in vitro
Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in porous hyaluronic acid scaffold
Antibacterial activity within degradation products of biological scaffolds composed of extracellular matrix
Pre-publication history
The pre-publication history for this paper can be accessed here:


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Greetings from London.
I thought I'd share the below quote, which was sent to me (Thanks, Thao!) when I was considering whether or not to put up the controversial FISA post. I knew beforehand that it would lose me some readers.
The few words below cut through hours of meaningless deliberation and made the decision simple…
There are a lot of hard choices and big decisions in life. Dealing with most of them requires facing daunting — but transient — discomfort.
I encourage you to read the following, which helped me to eliminate the rationalization we so often use to avoid doing what is important.
"I say to you, this morning, that if you have never found something so dear and precious to you that you will die for it, then you aren't fit to live.
You may be 38 years old, as I happen to be, and one day, some great opportunity stands before you and calls upon you to stand for some great principle, some great issue, some great cause. And you refuse to do it because you are afraid.
You refuse to do it because you want to live longer. You're afraid that you will lose your job, or you are afraid that you will be criticized or that you will lose your popularity, or you're afraid that somebody will stab or shoot or bomb your house. So you refuse to take a stand.
Well, you may go on and live until you are ninety, but you are just as dead at 38 as you would be at ninety.
And the cessation of breathing in your life is but the belated announcement of an earlier death of the spirit.
You died when you refused to stand up for right.
You died when you refused to stand up for truth.
You died when you refused to stand up for justice."
-Dr. Martin Luther King, Jr.
From the sermon "But, If Not" delivered at Ebenezer Baptist Church on November 5, 1967.
Posted on: September 4, 2008.
Please check out Tools of Titans, my latest book, which shares the tactics, routines, and habits of billionaires, icons, and world-class performers. It was distilled from more than 10,000 pages of notes, and everything has been vetted and tested in my own life in some fashion. The tips and tricks in Tools of Titans changed my life, and I hope the same for you. Click here for sample chapters, full details, and a Foreword from Arnold Schwarzenegger.


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Buy Videocon VJU32HH08CAM 32 inch HD Ready LED In India at These Prices
Videocon VJU32HH08CAM 32 inch HD Ready LED Full Features & Specifications
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Welcome to the ineedhits Search Engine Marketing blog, where we share the latest search engine and online marketing news, releases, industry trends and great DIY tips and advice.
There's been no end to the speculation over the future of the former search engine butler over the last few weeks. And really there have been few clear answers from the ranks at Ask.com or the IAC – well until now.
Ask.com spokesman and VP, Nicholas Graham has been vigilantly watching all the speculation and putting out the spot fires that blog rumors have created. He even popped over to our post on Ask.com's plans for Teoma last week.
Needless to say he's been a busy man.
In the fallout of some recent layoffs and obvious cost cutting, Graham has gone out and finally shared Ask.com's plans and re-aligned focus.
According to an Interview with Jordan McCollum from WebProNews, Graham has this to say:
"The idea that we're going to become a 'women's site' is not correct…
…what we've found from our research is that there's a sizeable group of our core user base that is women. This core audience comes for certain types of searches, certain categories-reference (dictionary, thesaurus, etc.), health and entertainment. Our new strategy is to focus on building up our ability to render good answers in those categories for our core audience…
…the idea that we're not going to be a search engine anymore is not correct. We'll continue to cater to all sorts of people who come to search… We'll continue to be a great search engine, but a great search engine with a new focus."
So for all our readers that are passionate fans of ask.com as a search engine – rest assured, Ask.com will remain with a strong commitment to being a relevant source of answers.
And on whether they still have Google in their sights:
According to Graham they're "not giving up on bettering Google", they're simply focusing on being a more relevant place for people to find answers and being "best at that".
It's been a turbulent few weeks for Ask.com and hopefully this clear direction will help them concentrate their efforts on an important core audience and create a sustainable advantage over their competitors.
Keep up to date with the latest from our blogs.
AOL Set to Swamp us With 30 New Websites
It seems that AOL wants to make 2008 its best year ever after they recently announced that they want ...


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Home > Resources > Retrieve Android Contacts
How to Recover Deleted Contacts from Android Phones?
Many people ask about how to recover lost contacts on Android phone since such phone data is quite easy to be lost from a mobile phone. I have ever wanted to clear the cache of the contacts storage app in order to free up my phone space. But accidentally I deleted all the contacts and sadly I don't have a backup of them. I did my best to find a solution to recover all the deleted contacts on my Android phone. And nothing is impossible for a willing heart, I finally found the Vibosoft Android Contacts Recovery.
This Android Contacts Recovery allows you to recover deleted, lost, formatted or corrupted contacts on your Android mobile phone directly from the phone or SIM card on the phone. Not only retrieve Android contacts, you can also use this marvelous tool to get back text messages, videos, photos and more from Android phone without any quality loss. All the recovered files will perfectly keep the original quality. Now, let's go through how to recover contacts from Android mobile by using Android Contacts Recovery.
Before that, you need to free download the program on your computer.
Guide to Retrieve Android Contacts (Phone Numbers) in 3 Steps
Step 1: Run the program and enable USB debugging on your Android
After connecting your Android phone to the computer, launch the installed Android Contacts Recovery. Check if you have enabled the USB debugging on your phone. If not, you should follow the below instructions to enable it at first. Choose the right way for your own device.
1) For Android 2.3 or earlier: Enter "Settings" < Click "Applications" < Click "Development" < Check "USB debugging"
2) For Android 3.0 to 4.1: Enter "Settings" < Click "Developer options" < Check "USB debugging"
3) For Android 4.2 or newer: Enter "Settings" < Click "About Phone" < Tap "Build number" for several times until getting a note "You are under developer mode" < Back to "Settings" < Click "Developer options" < Check "USB debugging".
Click the button of "Allow" on your phone after enabling the USB debugging on your phone.
Step 2: Scan your Android phone for lost contacts
You will get the scanning results on the left. Click on the "Contacts" folder and all the contacts, together with detailed information will can be seen on the right. Preview and check those you want to get back.
Step 3: Restore deleted contacts on Android selectively
Ready to proceed with the last step? It is quite simple, just click on the "Recovery" button and then, all the selected Android contacts will be saved in HTML and CSV on your computer.
Read More:


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Roberts, 50, seems assured of confirmation because several members of the "Gang of 14," a group of moderate Democratic and Republican senators whose support would be needed to block his nomination, say they don't think such an effort would be justified. "I can't imagine a filibuster," said Sen. Ben Nelson, a Nebraska Democrat. Neither can Sen. John Warner of Virginia, the group's senior Republican, who says he has talked with others in the group.
A filibuster is a maneuver that allows a minority of senators to block a vote. Because Republicans hold 55 of the Senate's 100 seats, Roberts' opponents say it's probably the only way to stop him. "I can count," said Eleanor Smeal, president of the Feminist Majority Foundation.
It takes 41 votes to sustain a filibuster, and there won't be enough without the support of the seven Democrats in the Gang of 14. Even some of the eight Democrats on the Senate Judiciary Committee who sparred with the nominee this week told him they were at least considering a vote in his favor.
Those committee members are among the chamber's most liberal Democrats, and several have reason to court liberal activist groups: Sens. Joseph Biden of Delaware and Russ Feingold of Wisconsin are weighing bids for the presidency in 2008; Sen. Charles Schumer of New York is trying to solicit funds for 2006 candidates as head of the Democrats' senatorial campaign committee.
An important factor in the Democrats' strategy: how best to position themselves for the next Supreme Court debate. The president has said he will act in "a timely manner" to fill the seat of retiring Justice Sandra Day O'Connor, a critical vote on the often divided nine-member court. Because O'Connor is a moderate conservative who — unlike the late Chief Justice William Rehnquist — often voted with the court's four-member liberal wing, her seat affects the court's direction.
Democrats such as Senate Minority Leader Harry Reid of Nevada want a moderate nominee like O'Connor. Some prominent women, including O'Connor, have suggested that Bush pick another woman. Bush has hinted he might make his attorney general, Alberto Gonzales, the first Hispanic justice. Conservative Republicans such as Sen. Sam Brownback of Kansas want a justice more obviously opposed to abortion and gay marriage than Roberts has appeared to be.
Bush was rebuffed this year when Republican leaders tried to end filibusters of judicial nominees, a move that would have made it easier to win approval of conservative appointees. The Gang of 14 consists of seven Democrats who promised to use a filibuster only in "extraordinary circumstances" and seven Republicans who back Democrats' right to filibuster.
Democrats in the group want what Sen. Mark Pryor of Arkansas calls "a nominee who will unite us, not divide us." They hope Bush will consult them, as he did before nominating Roberts. Sen. Mary Landrieu, D-La., said Bush aides asked her about Edith Clement, a judge on the U.S. appeals court that covers Louisiana, Texas and Mississippi. Clements' lack of controversial writings on abortion could make her an appealing candidate.
"She would be very high in my book," Landrieu said.


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What To Expect
Wander through River Safari, one of the world's largest repositories for river dwelling animals, and come face-to-face with the ecosystems of the world's most iconic rivers!
Inspired by the world's iconic river habitats (the Mississippi, Congo, Nile, Ganges, Mekong, Yangtze and the Amazon), the park is home to 200 land and aquatic animal species and is Asia's first and only river-themed wildlife park.
Stroll along freshwater aquariums and luscious walk-through exhibits, including attractions such as the Giant Panda Forest and the Amazon Flooded Forest, the world's largest freshwater aquarium.
End your River Safari visit with a 10 to 15 minute boat ride down the Amazon River* before enjoying some free time which you can spend around the park before starting the Night Safari adventure.
Set in 40 hectares of secondary jungle, the world's first Night Safari will allow you to experience the tropical jungle after dusk with stunning effects.
See a myriad of nocturnal animals, from fierce predators to timid forest dwellers, in their natural habitat under the subtle glow of the moonlight. With priority boarding, you won't have to wait in line to board the Tram Safari which takes you through the East and West Loop. Pass by a large reservoir and weave through selected habitats designed specifically to replicate the natural environment from the Himalayan Foothills to the Southeast Asian Rainforest and Indian Subcontinent.
Let the guide take you on a Walking Trail to view some animals at close quarters. There will also be an opportunity to catch either the Thumbuakar Performance of fire-eating skills OR a 20 minute animal extravaganza at the Creatures of the Night Show, subject to weather conditions.
Pick-up and drop-off service from major Singapore hotels
River Safari admission ticket
River Safari Amazon River Quest (or River Safari Cruise) boat ride*
Free use of WiFi inside the park
Free Downloadable Audio Guide (River Safari Adventure Buddy App)
Night Safari admission ticket
Night Safari tram ride priority boarding
Night Safari Creatures of the Night animal show or Thumbuakar Performance fire show**
Meals and drinks
Tips and gratuities
Personal expenses
*Amazon River Quest may be replaced by the alternative River Safari Cruise during adverse weather conditions. Boat rides are subject to weather conditions and height requirements and other restrictions. Children must be at least 1.06 meters in height. Children between 1.06 to 1.35 meters must be accompanied by an adult.
**Night Safari Creatures of the Night animal show and Thumbuakar Performance fire show are subject to weather conditions.
This tour has a 2 passenger minimum requirement for operation and is subject to cancellation if this minimum is not met. Please call at least 24 hours prior to departure to confirm schedule.
Tour duration includes traveling time to and from the attraction. After the morning River Safari visit, guests will have some free time before the Night Safari Tour starts.
Children below 2 years old are free of charge.
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|Battle of Tannenberg
Russian infantry advancing on Prussian positions during the Battle of Tannenberg.
|May 7 - May 12, 1954
|Decisive Prussian Victory
The Battle of Tannenberg was a decisive engagement between the Russian Empire and Prussia in the early days of the Great War, fought by the Russian First and Second Armies and the Prussian Second Army between 7 May and 12 May 1954. The battle resulted in the almost complete destruction of the Russian Second Army.
Worried about his loss at Gumbinnen and the continued advance of the Russian Second Army to the south, Prittwitz ordered a retreat to the Vistula, effectively abandoning eastern Prussia. When he heard this, the German Army Chief of Staff, recalled Prittwitz and his deputy Waldersee to Berlin. They were replaced by Heinz Guderian, called out of retirement, with Erich Von Manstein as his Chief of Staff.
The Battle
1. The Initial moves
2. The Armoured drive
The Prussian A5V Panzers were now unleashed upon the unsuspecting Russians. Led by Manstein Hiomself, who led from the command Panzer,the prussian forces broke through the russian lines and killing anything in there path.
3. The collapse
Rather than report the loss of his army to the Czar, Yeremenko committed suicide by shooting himself in the head on 13 May 1954.


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This item from Bonnie Boswell Antiques is now SALE PENDING.
More from Bonnie Boswell Antiques
Vintage World War Two Silver 3 Pence Coin Sweetheart Bracelet George V - VI
We are pleased to present this Vintage World War Two Silver 3 Pence Coin Sweetheart Bracelet circa: King George V - VI. Is there anything in the jewelry world hotter than coin jewelry? NO! The perfect example of what's old is new again. This is a nice World War II era Sweetheart Bracelet. These coin bracelets were referred to as sweetheart bracelets because metal was scarce during the war. This has 6, 3 pence coins with George V and George VI. George V reigned from 1910-1936 George V1 from 1936-1952 There are 3 coins of each king. The back side of the coin is covered with a plaque the coin was set upon. I can only read one date 1935. It is my understanding that the 3 pence coins of this vintage are sterling silver?
CONDITION: as presented. I have lightly polished the coins but suspect they would clean and polish better. We all have our own feelings about patina
Gender: Female, Age Group: Adult, Color: silver, Size: 7 1/4"
If you are the pending buyer of this item, go to My Account to view, track and pay for this item.
Bonnie Boswell Antiques
Bonnie Boswell
Whitefield, NH
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The Ted Baker Travel Lifestyle Organiser will keep your documents and personal items safe and secure whilst you are travelling. Crafted from soft, durable faux leather in blush pink with a brogue style of patterned perforation and a rose gold zip, the organiser has Ted says 'work it' in gold script embossed on the front. Opening to reveal compartments and elasticated bands, the Thistle Organiser comprises a card holder, paper pocket, pen holder, mobile compartment, headphone clamp and a detachable pencil case. Presented in a stunning Ted Baker branded gift box, the sophisticated case will enhance your travelling experience.
|Size / Fit:
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Attention Publix shoppers! Select zip codes will be getting a $5 off $40 Publix coupon in their local Tampa Bay Times, Tampa Tribune, Lakeland Ledger, Bradenton Herald, Sarasota Herald Tribune and Naples News-Press on 4/17/16.
Even though I live in Pinellas County, I have yet to encounter these single page flyer inserts at any of my local stores since they started doing the promotions in "select" zip codes. Make sure you check the newspapers before buying them.
The coupon is good through 4/23/16 in these Florida counties: Pasco, Pinellas, Hillsborough, Manatee, Sarasota, Charlotte, Lee, Collier, Lake, Sumter, Highlands and Polk.
Good luck!
Thanks True Couponing.Extreme Couponing Tip: You can usually get two prints of most internet coupons. To find them in the easiest possible way, use our preclipped links that take you right to the coupon. Then come back and click the same link to get your 2nd print. This will save you the time spent searching for the coupon.
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Call us on:
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We are sorry that we do not have the opportunity to work with cats as our cats are fostered in the homes of our fosterers and we do not have a rescue centre. If you are interested in hands-on experience please try Cats Protection Homing Centre in Mitcham.
Fundraising Events Organiser
As a Fundraising Events Organiser, you will play a key role in co-ordinating, motivating and supporting local volunteers in raising those all-important funds. Cats in our care rely on generous donations from people who love cats and count on volunteers like you to make those donations happen. You will lead volunteers in a variety of fundraising events and activities raising much needed funds for cats in your area.
Cat Fosterer
Cats Protection helps thousands of cats across the UK each year thanks to our network of dedicated volunteers. As a fosterer you will be directly helping cats and kittens in need; providing them with hands on care and working with others
to rehome them, ultimately, providing them with a second chance of a happy and healthy life.
Cat Fosterer Support
Could you help support the Fosterers by transporting cats to and from the vets and collecting stray cats and delivering them to the vets. This would be on an ad hoc basis and you can decide how much time you are able to spare.
Education Speaker
As an Education Speaker you will act as an ambassador for Cats Protection and play a key role in raising awareness of how the charity makes a big difference to the lives of thousands of cats and kittens in need each year. You will inspire others, develop learning and understanding of feline welfare and be the voice for cats in your community.
Do you have contacts in the local press? If so we would love your help to maximise our coverage in the local papers and radio by creating press releases and contacting journalists.


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Discover a selection of top forums scuttle lakeside on the internet. Come to share your passion on forums scuttle lakeside and meet other fans of scuttle lakeside. Then, why don't you create your own forum of scuttle lakeside ?
Lakeside Warriors is a RP site based off of the Warrior series by Erin Hunter.
#lakeside, warriors, site, based, warrior, series, erin, hunter


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L. R. Scarborough
In "The Searchlight" of November 13th Dr. Norris misses the Exodus code or morals a long ways. I give two or three examples of how he has missed it.
1. He seeks to make the impression, by certain deeds of record, that the Seminary was located in Fort Worth, on Seminary Hill, about March 9, 1910. The facts are that the bonus of $100,00.00 was raised and the gifts of land were made in October. The trustees unanimously, after considering a number of locations, without influence either from Dr. Carroll or from me, located the Seminary at its present place in November 1909. A plot and maps of the land were made and architects employed in December. I moved here early in December to take charge and put up the buildings. There were five pieces of land deeded to the Seminary. Two of them were deeded in December, one of them early in January. The other two, because of certain complications and conditions made in the gifts, where trusteeships had to be established and certain conditions worked out, were not finally made until January 24th. The lawyers and committee had several meetings, took them thirty days to work it out. A contract was let for Fort Worth Hall January 4th and the building was begun early in January, before I purchased the little plot of land that Dr. Norris is turning somersaults over. Dr. Carroll and other members f the faculty selected and contracted for the lots they bought before or about the time I did mine. The records show that the Seminary was located by trustee action months before I purchased the little plot of land. The purchase I made of this land had no more to do with the locating of the Seminary on its present site than buying a piece of green cheese his to do with the time the moon will rise.
2. Dr. Norris says he is going to the Southern Baptist Convention and see to it that the books of the Nashville headquarters, during the 75 Million Campaign, are audited. He need not trouble himself about this. Dr. Van Hess was treasurer of that fund and handled all the money and his books have been audited six or seven times and unanimously apporved every time by the Southern Baptist Convention. Dr. Norris tried to make the insinuation that the Southwestern Seminary got more money out of the 75 Million Campaign than it should have. He is in error there. I never received any of the 75 Million Campaign money except that paid on my salary. He intimates that I got two salaries, one from the Campaign and one from the Seminary. The records show that I received money from both of these sources, but never at the same time. Not one day was there any lapping of salaries. All the money the Seminary received out of the Campaign was either sent by the state secretaries or by the Education Board secretary. They had definite percentages on which to divide this money and if we got more than we should they are responsible. All these secretaries are honorable men aand their reports have been audited several times; but if there is any blame attached and if the Seminary got more than its share those who divided and sent the money are to blame. I know the Southwestern Seminary got only its percentage part as was agreed on by the Executive Committee of the Southern Baptist Convention before I was made Director of the Campaign.
3. Dr. Norris would make the people believe that the seminaries had done wrong in borrowing money from the boards and not paying it back. The Southern Baptist Convention unanimously at Washington instructed these boards to loan this money to the seminaries; but it was definitely stated that the boards were to be refunded this money either out of money collected over and above the 75 Million or out of future campaigns. If there is any wrong done in this matter, the Southern Baptist Convention did it and did it unanimously.
4. Dr. Norris prints a photographic copy of a letter from a pastor in Florida. In this letter I am accused of expelling a son of this pastor on the grounds that he refused to quit singing in the choir of the First Baptist Church and attending the preaching of Dr. Norris. The minutes of the faculty of the Seminary show that this young minister was expelled because of charges made against him by his wife and the people they lived with. He confessed his guilt before the faculty. The cause was sufficient for explsion. He afterwards repented and begged forgiveness and on a certain plan was taken back on probation. His divorced wife could give some interesting testimony in this cause. This pastor says that I have hounded his son ever since. This is a mistake. I have only tried to collect the $200.00 security note which I had to pay for the boy, endorsed by the father. In the light of the facts in the case, it is strange that any minister could write the letter printed in "The Searchlight". It is not strange that "The Searchlight" would print the letter. Neither I nor the faculty of the Seminary know that htis boy ever attended the First Church or sang in the choir until we saw it in the father's letter. There is no truth in that charge.
This is only another case where people have been disciplined by the Seminary for bad conduct or dismissed on other delinquencies who have immediately gone to Dr. Norris for refuse. This will explain some of the affidavits and letters which Dr. Norris publishes.
These are but samples of "The Searchlight" misrepresentations. It is useless to answer any of the others. The brethren had just as well know once for all that "The Searchlight" is not a factory for the truth.
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Crazy Images Photographer
JessicaFabacModel Female Austin, Texas, US
My MM URL: http://www.modelmayhem.com/JFabac
Mayhem # 3045992
About MeI have been modeling for about a year now including a few fashion shows, and a couple promotional jobs. I have currently just graduated college, and now that I am no longer in school full time I am looking to shoot much more! I have worked with some great people, and am still learning. I am looking to expand my portfolio, as I strive to continue learning and doing different types of shoots.
As for me personally I am athletic and have loved sports my whole life. I never thought I would get into modeling as I had opportunities as a child, but was focused on my sports. As I have gotten older and experienced my first shoot I have fallen in love and get excited with each shoot. I feel very strongly about keeping things professional, but at the same time I am goofy, and love to have fun with all my shoots.
I have many ideas and concepts that I have been wanting to try and am looking forward to collaborating with new photogs, hairstylists, MUA, etc.
NO NUDE SHOOTS. Will do implied if I feel comfortable
Feel free to contact me with any questions, or ideas! :)
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Do you remember during the prior Presidential Administration–back when the President was evil–when we fought "illegal wars" approved by and repeatedly funded by Congress?
Do you remember when some claimed that the "missing" WMDs weren't missing, just moved? Do you remember in response Bashir Assad denying that Syria had any chemical weapons at all?
Syrian President Bashar Assad's regime has used chemical warfare in order to ease its entrance into Homs, said Awad Al-Razak, an officer who defected from the Syrian armed forces.
Al-Razak, who served in the chemical warfare department of the Syrian military, told the Al-Arabiya network that the government used nerve gas under the supervision of Russian and Iranian scientists, and intends to do so again in other parts of the country.
I'm not surprised at all that Assad has turned out to be the kind of monster that would use chemical weapons, in a desperate bid to remain in power, if that is in fact what occurred. Nor would I be surprised if Iran, who has used chemical weapons before, chose to assist in deploying such weapons to one of their last remaining friends in the region. I am a little surprised that Russia would be clumsy enough enough to have their fingerprints on an operation like this.
Frankly, it doesn't matter if the weapons used were from Saddam's Iraq, current Iranian stockpiles, or of Syrian manufacture. Syrian civilians are just as dead, and Assad is courting a war crimes prosecution or summary execution whether the weapons he is using against his own people are a nerve agent or "just" high explosives.