text:
A deterministic map of Waddington's epigenetic landscape for cell fate specification
The image of the "epigenetic landscape", with a series of branching valleys and ridges depicting stable cellular states and the barriers between those states, has been a popular visual metaphor for cell lineage specification - especially in light of the recent discovery that terminally differentiated adult cells can be reprogrammed into pluripotent stem cells or into alternative cell lineages. However the question of whether the epigenetic landscape can be mapped out quantitatively to provide a predictive model of cellular differentiation remains largely unanswered.
Here we derive a simple deterministic path-integral quasi-potential, based on the kinetic parameters of a gene network regulating cell fate, and show that this quantity is minimized along a temporal trajectory in the state space of the gene network, thus providing a marker of directionality for cell differentiation processes. We then use the derived quasi-potential as a measure of "elevation" to quantitatively map the epigenetic landscape, on which trajectories flow "downhill" from any location. Stochastic simulations confirm that the elevation of this computed landscape correlates to the likelihood of occurrence of particular cell fates, with well-populated low-lying "valleys" representing stable cellular states and higher "ridges" acting as barriers to transitions between the stable states.
This quantitative map of the epigenetic landscape underlying cell fate choice provides mechanistic insights into the "forces" that direct cellular differentiation in the context of physiological development, as well as during artificially induced cell lineage reprogramming. Our generalized approach to mapping the landscape is applicable to non-gradient gene regulatory systems for which an analytical potential function cannot be derived, and also to high-dimensional gene networks. Rigorous quantification of the gene regulatory circuits that govern cell lineage choice and subsequent mapping of the epigenetic landscape can potentially help identify optimal routes of cell fate reprogramming.
The biologist Conrad Hal Waddington, in the course of a career spanning four decades (1930s - 1970s), attempted a bold synthesis of the fields of genetics, embryology and evolution
Figure 1
Mapping Waddington's epigenetic landscape
Mapping Waddington's epigenetic landscape. (A) The "epigenetic landscape" proposed by Conrad Waddington shows a ball rolling down valleys separated by ridges on an inclined surface, as a visual metaphor for the branching pathways of cell fate determination. Figure reproduced from original text by Waddington
In the quantitative view of a cell as a dynamical system governed by genetic interaction networks
Huang, Wang and colleagues have recently proposed a probabilistic "pseudo-potential" to quantify the epigenetic landscape for a gene network regulating cell fate, where the elevation of the surface is inversely related to the likelihood of occurrence of a particular state in phase space
Here we propose a simple numerical method to map the epigenetic landscape that is not based on a probabilistic or master-equation approach. Instead, a quasi-potential surface (Figure
Finally, we discuss ways in which this quantitatively mapped landscape may help predict the efficiency of cellular de-differentiation or trans-differentiation, and identify optimal routes of cell fate reprogramming. Recent discoveries have challenged the dogma of cell fate determination as a unidirectional and irreversible process. Even terminally differentiated adult cells have now been shown to retain considerable phenotypic plasticity and the ability to be reprogrammed into pluripotent stem cell-like states
Results and Discussion
Derivation of the quasi-potential landscape
We first illustrate our quantitative approach with a simple circuit of two genes
If we were able to derive a closed-form potential function
then the local minima on the two-variable potential surface
In general, condition (3) will not be valid for an arbitrary circuit of two genes
Therefore, given that a gene circuit is in general a
Figure 2
Computing the epigenetic landscape for a bistable switch based on a double-negative feedback circuit of two genes
Computing the epigenetic landscape for a bistable switch based on a double-negative feedback circuit of two genes
where Δ
The change in the quasi-potential, Δ
For positive increments in time Δ
The procedure described above was repeated to evaluate the change in the quasi-potential along trajectories originating from different points in
Figure 3
Ridges and valleys on the computed epigenetic landscape of a bistable (A, B) and a tristable (C, D) regulatory network of two genes
Ridges and valleys on the computed epigenetic landscape of a bistable (A, B) and a tristable (C, D) regulatory network of two genes
The same procedure can be applied to systems with more than two stable steady states - for instance, a "tristable" system produced by a circuit of two genes that induce their own expression, in addition to mutual inhibition (Figure
Quantitative interpretation of the quasi-potential landscape
To establish that the "elevation" of the computed landscape at a given location in
Figure 4
Valleys on the computed epigenetic landscape represent high-occupancy stable steady states, while ridges represent barriers to stochastic transitions between those stable states
Valleys on the computed epigenetic landscape represent high-occupancy stable steady states, while ridges represent barriers to stochastic transitions between those stable states. For the tristable two-gene system, increasing the Hill coefficient
Figure 5
Height and steepness of barriers affects stochastic occupancy of stable states
Height and steepness of barriers affects stochastic occupancy of stable states. (A) Percentage of stochastically simulated cells in the three attractors
The "third dimension" (elevation) of the landscape represented by the quasi-potential, although directly derived from the dynamic rate equations without any additional information, thus yields an interpretation of cellular stability not immediately apparent from two-dimensional phase portrait analysis. The analysis above supports the contention that the length of the "least action trajectory" along the contours of the epigenetic landscape is more important in predicting transitions between alternative cellular states than the simple "aerial distance" in state space
These results suggest that calculating the relative heights of the ridges and valleys on the computed epigenetic landscape of a multi-gene system can help predict the probability of trans-differentiation from one cell lineage to another, or de-differentiation of a particular cell type to its progenitor state. Current efforts to reprogram cell fate with potential application in regenerative medicine suffer from a low rate of successful reprogramming
Additional file 1
Supplementary Figures. This file includes additional figures to supplement the text.
Click here for file
A dynamic landscape
The computed epigenetic landscape derived above should not be interpreted as a static surface
Figure 6
The shape of the computed epigenetic landscape can be altered by modifying gene interaction parameters
The shape of the computed epigenetic landscape can be altered by modifying gene interaction parameters. When basal expression
Interestingly, this flexibility of the quasi-potential surface under gene manipulation gives a quantitative interpretation of the revised image of the epigenetic landscape proposed by Waddington (Figure S3, Additional File
In this work, we have defined a deterministic quasi-potential that is minimized along a temporal trajectory followed by a gene network, and used it to quantitatively derive the corresponding epigenetic landscape. A gene network not being a mechanical system, this quasi-potential should not be confused with a potential energy function. It is rather a Liapunov function of the dynamical system represented by the gene network, along which trajectories flow monotonically "downhill" towards the steady states of the network
This novel and simple process for deriving the surface of the landscape from a path-integral quasi-potential is not restricted to two-gene systems. While the landscape cannot be visually rendered for circuits with more than two genes, the rates of transition across the potential barriers between multiple steady states in the system can still be computed to predict optimum routes of cell fate reprogramming.
However, many binary branching points in development, particularly in blood cell lineage specification, are governed by mutual antagonism of only two transcription factors associated with alternative lineage choices
Bistable network model
To illustrate the derivation of the epigenetic landscape, we used a simplified mathematical model of a bistable network of two genes,
where variables
Tristable network model
The tristable network model consisted of two genes,
where the new parameters
Integration Algorithm
To evaluate the change in the quasi-potential along each trajectory in
Thereafter, at each time step:
• The rates
• Expression levels
where for increments in time Δ
• The quasi-potential
The above steps were repeated until the quasi-potential
Software platforms used
The deterministic models were implemented and simulated on the MATLAB® (R2009a, The MathWorks, Inc., Natick, MA) platform, while the BioNetS program
Visualization of stochastic simulation results
The stochastically simulated "cells" (i.e. individual realizations of the stochastic network model) were overlaid on the quasi-potential surface at the
Additional file 2
Supplementary Model Code. This file lists the source code in MATLAB® format for the computational algorithm used to derive the epigenetic landscape.
Click here for file
Authors' contributions
SB designed the study, constructed the computational model and performed computer simulations, and wrote the paper. QZ participated in study design. QZ and MEA discussed the results and commented on the manuscript. All authors read and approved the final manuscript.
We thank J. D. Schroeter, C. Woods, R. B. Conolly, H. J. Clewell III, J.E. Trosko, M. Thattai, J. M. Haugh and B. Howell for critical discussions and reading of the manuscript. This work was supported by the Superfund Research Program of the U.S. National Institute of Environmental Health Sciences, and by the American Chemistry Council.
Epigenetic landscaping: Waddington's use of cell fate bifurcation diagrams
Conrad Hal Waddington: the last renaissance biologist?
An introduction to modern genetics
Organisers and Genes
The Strategy of the Genes
Metabolic Stability and Epigenesis in Randomly Constructed Genetic Nets
Stem Cell States, Fates, and the Rules of Attraction
Reprogramming cell fates: reconciling rarity with robustness
Systems biology of stem cell fate and cellular reprogramming
Structural Stability and Morphogenesis
An Inventory of Waddingtonian Concepts
The Potential Landscape of Genetic Circuits Imposes the Arrow of Time in Stem Cell Differentiation
Understanding gene circuits at cell-fate branch points for rational cell reprogramming
Potential energy landscape and robustness of a gene regulatory network: Toggle switch
Optimal enumeration of state space of finitely buffered stochastic molecular networks and exact computation of steady state landscape probability
Robustness, stability and efficiency of phage λ genetic switch: Dynamical structure analysis
Calculating biological behaviors of epigenetic states in the phage λ life cycle
Intrinsic noise, dissipation cost, and robustness of cellular networks: The underlying energy landscape of MAPK signal transduction
Potential landscape and flux framework of nonequilibrium networks: Robustness, dissipation, and coherence of biochemical oscillations
Potential Landscape and Probabilistic Flux of a Predator Prey Network
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
Induction of pluripotent stem cells from adult human fibroblasts by defined factors
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution
Induced pluripotent stem cell lines derived from human somatic cells
Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons
Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency
Molecular basis of transdifferentiation of pancreas to liver
Stepwise reprogramming of B cells into macrophages
Conversion of mature B cells into T cells by dedifferentiation to uncommitted progenitors
In vivo reprogramming of adult pancreatic exocrine cells to beta-cells
Direct conversion of fibroblasts to functional neurons by defined factors
Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors
Direct conversion of C. elegans germ cells into specific neuron types
Extreme Makeover: Converting One Cell into Another
Elite and stochastic models for induced pluripotent stem cell generation
Forcing cells to change lineages
Non-genetic heterogeneity of cells in development: more than just noise
Epigenetic reprogramming and induced pluripotency
Construction of a genetic toggle switch in Escherichia coli
Nonlinear Dynamics and Chaos: With Applications to Physics, Biology, Chemistry, and Engineering (Studies in Nonlinearity)
Bifurcation dynamics in lineage-commitment in bipotent progenitor cells
Stochastic modelling for quantitative description of heterogeneous biological systems
Stochasticity in gene expression: From theories to phenotypes
Stem cell biology meets systems biology
Noise-based switches and amplifiers for gene expression
Enhancement of cellular memory by reducing stochastic transitions
Towards an understanding of lineage specification in hematopoietic stem cells: A mathematical model for the interaction of transcription factors GATA-1 and PU.1
Computational Modeling of the Hematopoietic Erythroid-Myeloid Switch Reveals Insights into Cooperativity, Priming, and Irreversibility
Biochemical Network Stochastic Simulator (BioNetS): software for stochastic modeling of biochemical networks
A general method for numerically simulating the stochastic time evolution of coupled chemical reactions
Exact Stochastic Simulation of Coupled Chemical-Reactions


text:
Thank you for your interest in Immaculate Conception Cathedral School. As you explore our website, we hope you'll get a sense of our mission and the spirit that has filled our Central Gardens campus for 90 years.
We encourage you to bring your family to step onto our grounds and into our classrooms. When you do, you will quickly realize that we look very familiar. Of all the Memphis-area private schools, ICCS truly reflects our city and our world. We are diverse, whether you're measuring ethnic background, geographic origin, academic ability, faith tradition or zip code.
Since 1922, our students have gone on to shine brightly in their communities and in countless endeavors. We know that you are looking for the campus environment and faculty connection that works best for your child. I invite you to spend some time with us at ICCS and consider the possibilities.
Msgr. Valentine "Fr. Val" Handwerker, Rector
The Cathedral of the Immaculate Conception

subset: commoncrawl


text:
Koozies (also particular as can coolers) are wonderful to have on a picnic, or once you're righteous seated external on a hot day. They hold on to your drinks colder for noticeably longer. Koozies are a great invention, but have you of all time stopped and deliberation roughly how they work? The quality of their ornamentation and its power is imaginative because it makes use of simple physical science principals.
We all cognise that once you put impressively cool h2o in a room, and let it sit, it will gradually move to freedom heat. The same consequence is found once you set thing completely hot in a area (it will leisurely put on ice off to liberty heat). The reasons at the rear this pb us to take in how koozies donkey work. It all has to do beside the motility of atoms.
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Voyeur : December 2010
virgin blue group news DECEMBER 019 BRISBANE YOUTH SERVICE Angela Barnes, the 34-year-old executive manager of Brisbane Youth Service (BYS), has been involved in helping homeless and vulnerable young people for more than 15 years. How does BYS help the community? BYS works with homeless and other wise disadvantaged young people aged between 12 and 25 years. Today, we have more than 40 staff responding to situations concerning people's immediate needs, such as food, emergency accommodation and showers, through to intense ongoing support and structure programs. Why did you become involved? From when I was 15 years old I knew I wanted to work with young people who needed more positive life experiences. This led to undertaking a degree in psychology and working with kids with very high-need disabilities. I followed this with an honours degree to be registered as a psychologist and running a heroin treatment program. Then I took my first role at BYS as a drug intervention worker. What are some of the biggest challenges that you and BYS face? Issues of inequality are probably the hardest thing to change. How do you help a young person imagine they could go to university if no-one in their house or life has ever even had a job? What else could the organisation do to help if it had more funds? Our biggest financial issue right now is trying to buy a building for vulnerable young people in inner-city Brisbane. Our last building, which we had for 13 years, was sold, so now we are in a commercial rental, which has no security of tenure. The list of what we need is extensive. What does the future hold for BYS? Hopefully we will soon achieve our dream of securing a space for young people. BYS is very good at what it does and it will, I believe, continue to grow and develop. Hopefully the number of young people who are homeless will decrease as more attention is given to this issue at the state and federal levels and we can focus on helping young people to live and not just sur vive. Brisbane Youth Service helps underprivileged youth realise their full potential and achieve their dreams. Virgin Blue is a proud supporter of Brisbane Youth Service. For more information visit www.brisyouth.org.
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Published: 2004/03/03
In the Moment
Published: 2004/03/03
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Jilu, is a region located in the province of Hakkari, Turkey. One of the oldest churches in the world, named Mar Zaia, is found in Jilu. The church was built in the 4th century AD. Before 1915 Jilu was home of Christian Assyrian from Jilu tribe.There were 17 Assyrian villages in this region,Village of Mar Zaya was seat of Mitropolit of the Church of the East,who was called Mitropolit of Jilu, Baz and Rekan.The last Metropolit whos seat was in Mata Mar Zaya was Mar Sargis Zaya. Jilwaye left their homeland in 1915 with other Assyrians from Hakkari and today they ancestors live all over the world.In Syria ,Khabur,there are two Assyrian villages with people from Jilu tribe. Today there are Jilvaye communities in Russia,Georgia,Iraq,Iran,Lebanon,Syria,USA,Australia,Europa,Cuba,Chile,Brazil
Characteristics of the "Jilu" Neo-Aramaic dialect
The Jilu dialect is one of the most distinct Neo-Aramaic dialects of Southeastern Turkey. It falls under the Northern Assyrian Neo-Aramaic group, (similar to the Bazi and Gawar/Gavar dialects). It has some elements of Turkish and Kurdish vocabulary and grammar.
Examples of common vocabulary and verb conjugations in the Jilu dialect:
|Urmian Standard
|Jilu Koine
|to come
|to go
|b'rikhsha or b'khasha
|come here!
|Syriac (male)
|Syriac (female)
|Jilu (male)
|Jilu (female)
|father's brother
|I will see you (to female)
|I will see you (to male)
|I want (male)
|E' bǎyihn
|E' bana
|I want (female)
|E' bǎyǎn
|E' bayan
|drink (command) (plural)
|you (plural)
|akhnokhu or akhtu
There exists also sub-tribes within the Jilu tribe, some with their own distinct pronunciation or verb conjugations. The Jilu read Urmian Standard based literature and read and write in Urmian Standard. Most Jilu can switch back and forth from Jilu to Urmian Standard when conversing with Assyrians of other tribes.
The Jilu dialect is said to "soften" Urmian standard Neo-Aramaic vocabuly terms (e.g. a softer "k" sound replaces the guttural "q" sound in qurbana and becomes "kurbana", see above table).
Verb conjugations
There are variances in the way verbs are conjugated in the Jilu dialect. Some speakers tend to adopt a form of verb conjugation that is closer to the Urmian Standard, this may be considered the Koine Jilu because it is the most widely used. The Koine Jilu may be attributed to the growing exposure to Urmian Standard based literature, media, and the liturgical language of the Assyrian Church of the East. The Jilu Koine may also be attributed to the social exposure of the Jilu to Assyrians of other tribes, especially to Assyrians speaking the Urmian dialect. The majority of Jilu Koine speakers are from/descendants of Jilu Assyrians from Iraq.
Some Jilu from Syria tend to have a verb conjugation system that is similar to the Jilu Koine but further from the Urmian Standard. This other dialect also has vocabulary terms that are foreign to Jilu Koine speakers from Iraq. Some speakers of Syrian(from Syria) Jilu claim that their dialect is more pure than that of the Iraqi speakers, although it is disputed.
Examples of verb conjugations,
|Urmian Standard
|Jilu Koine
|Syrian Jilu
|What is the matter with you (ppl)?
|malkhu or malokhu?
(It should be noted that speakers of Syrian Jilu are the descendants of the Jilu that relocated to Syria during the 1930s and 1940s from Iraq.)
Vowel pronunciation
Jilu of the B'Nehmaye tribe pronounce their "a"'s as "eh" when speaking.
|Urmian Standard
|Jilu Koine
|Jilu Koine - B'Nehmaye
Some Jilu sub-tribes (Most are named after the villages they resided in within Jilu)
- B'Smal
- B'Zkharyah
- Alisnaye
- B'Namaye
- B'Nehmitnaye
- Ishtaznaye
- Nehriknaye
- Nehraye
- Zeeryaya
- Yekmala
- Zarnaye
- B'Talanaye
(It should be noted that most "sub-tribes" are not actually sub-tribes of the larger Jilu Tribe itself. Rather each "sub-tribe" consists of Jilu members that come from a specific town within Jilu. Or, the name of the "sub-tribe" may come from a Jilu person's family name that resided in Jilu (e.g. "B'Skharyah" literally, "of Zechariah" ). Another possibility is that the sub-tribe name reflects the area or region the Jilu person's family originated from (e.g. "Nehraye" literally, "River dwellers").
All Jilu of the Jilu "sub-tribes" rather should be seen as a single unified tribe from different towns and regions within the Hakkari region of Jilu. Historically there was no distinction made between the Jilu people from the different towns, etc. All Jilu are of one Jilu tribe, a tribe of the Assyrians within the Ottoman Empire.
Famous Jilwayeh
- Albert Quryaqus - A founding member of the "Concerned Members of Mar Zaya Church"
- Sargon Dadesho - Creator and president of AssyriaSat
- Mar Sargis Yousip - Bishop of the Assyrian Church of the East in Baghdad, Iraq. Currently resides in Modesto, California.
- Mar Meelis Zaya (disputed Jilwa) - Bishop of Australia and New Zealand.
- Mar Iskhaq Yousip - Bishop of Nuhadra (northern Iraq) and Russia. Relative of Mar Aprim Khamis.
- Qasha Kando D. Kando - Former Qasha at Mar Zaya Towana Assyrian Church of the East in Modesto, California, USA. First cousin and brother-in-law of Mar Aprim Khamis.
- Malik Kambar d'Jilu - one of the Assyrian National Leaders in the beginning of the 20th Century
- David Adamov - President of the Assyrian International Congress of Georgia
- George Mansur - Assyrian singer from Sydney Australia.
- Malik Endreios - Malik of Jilu in early 1920's. He was deported with Mar Eshai Shimun XXIII. He was from Zerni village of Jilu. Grandson Awikan Shamasha Giwargis is still alive.
- Jimmy Giwargis- A founding member of the Mar Zaya Church Tel Koran, Syria. (narajhk.jeeran.com )
- Mar Rabban Al-Kass - Chaldean Beashop of Arbil and Amadiya
- Vladimir Kalamanov - Russian Federation representive in UNESCO
- Tony Gabriel - Assyrian Singer from Canada
List of the Assyrian villages of Jilu
- Alsan
- Medhi
- Nahra
- Zerini
- Mata d Mar Zaya
- Ummud
- Talana
- Be Bukra
- Nerik
- Ori
- Zer
- Serpel
- Bu Bawa
- Samsiki
- Mata d Oryaye
- Musperan


text:
A few months ago we released This Exquisite Forest, a Chrome Experiment that lets you create collaborative animations using an online drawing tool. Since then, thousands of people from all over the world have contributed to the project, creating unique animations like Looking Up / Looking Down, Wine after Coffee and Animated Typography. For any of these animations, you can click the button in the lower right to add to the story and branch it in a new direction.
Today, we'd like to share The Endless Theater, a new way to wander the forest by viewing a continuous stream of different animations. In addition, now you can embed animations directly into your site or blog, so it's even easier to share your work with the world. Just go into the lightbox view and click "Embed."
A project of this scale and diversity is really only possible on the web, where people can view and contribute from all over the world using only a browser. Thank you all for making the forest so full of life. Please continue to explore, find your favorite animations, and add to the story.
Posted by Aaron Koblin, Creative Lab
(Cross-posted from the Chrome blog)
Original: http://feedproxy.google.com/~r/blogspot/MKuf/~3/V95U73l9p-4/the-evolution-of-this-exquisite-forest.html
By: A Googler
Posted: December 11, 2012, 7:01 pm
Google incorporated founded in September 4, 1998, is a global technology company focused on improving the ways people connect with information.
The Company generates revenue primarily by delivering...


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Some of the better home security monitoring software allows you to ignore parts of the view screen so that you track only the motion you are interested in; for example, you may have a webcam set up to record your driveway but part of the recoding includes a busy street – with the right software, you can tell the monitoring software to block out the street portion and record only what you need.
At the time I started this page, there was not much in the way of security monitoring software, but that was years ago and today, just about every webcam has an associated software package that if free and as a result, this list has dwindled.
This home security monitoring software is completely free and works with and remote security surveillance camera, such as your basic webcam from Logitech, Microsoft, Labtech and others. This is excellent surveillance software and turns any webcam into a home security camera that allows you to detect motion, remote HTTP monitoring (http video streaming) and email you when your security camera detects motion. Although the software has not been updated in a while, it's still an excellent program.
Site: webcamspy.sourceforge.net
If you know of similar software that sound be included on this page, please leave a comment and we'll review.


text:
WynturStorm: "So I talked to your voice provider and she changed her mind. You are now allowed to be distributed under certain circumstances." Snow: "Thanks you master."
Name: Snow Utanen
Name interpretation: Snow- a random name that WynturStorm and Snow's voice provider felt fit her voice. Utanen- Uta- Song. ne-sound and the N at the end. Snow Song sound
resembles the Voice Provider's last name, which also ends in a N.
Type: SHASTALOID Model: 05
Voice range: C4 to C5 Alto
Gender: Female
Related Characters: Kuro Utanen (Younger Brother.), Star Utanen (Younger sister), Neko Utanen (Younger brother.), Shadow Utanen.(Younger Sister),Storm Otone (friend.).
(Fellow SHASTALOIDS, Neko isn't released yet.)
Age: 19
Genre: Any. Perferbly softer songs.
Homepage: https://www.facebook.com/shastaloid
Weight: ???
Character Item:???
Creator: shadowstar1021 on YouTube and DeviantArt. WynturStorm on Wiki.
Height: ???
Voice Source: Shadowstar1021 on Youtube's older sister..
Picture link List: http://shadowstar1021.deviantart.com/
Birthday: ???
Likes: ???
Media List: http://www.youtube.com/user/shadowstar1021
VoiceBank Download: http://www.4shared.com/zip/Coegvu3o/Snow_CV.html?
Release Date: June 13
Dislikes: ???
Signature Song: ???
Personality: She is the loving big sister who is willing to sacrifice any thing for the safety of her younger siblings. She can seem to be over protective but it is only because she loves her younger siblings. She lost her eye sight when she saved her youngest brother Neko from kidnappers.
Supplemental Information
Headgear: Head phones
Eye color: Grey (Blind)
Headphones: Grey and blue
Dress: A long white coat with blue trims. White pants. Grey boots with blue cuffs.
Nationality/Race: American Shastaloid.
Favorite phrase: ????
(Better Art work to come soon. More info to come.)
This has been verified as correct by the voice provider, the character design artist and the voice bank creator. ~WynturStorm/shadowstar1021


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Headlight Euro Guards, Black; 97-06 Jeep Wrangler TJ
Protect your headlights with this pair of black headlight euro guards from Outland Automotive. Fits 97-06 Jeep Wrangler (TJ).
Pricing: $25.99
When you go off-roading, you want to know that you have protection when and where you need it, especially near the vulnerable areas of your Jeep like your headlights! That's why at Outland Automotive, we cover your Jeep from top to bottom. These black headlight Euro guards guards not only dress up your Wrangler, but make sure they don't take a major trail beating! Easy to install with no drilling required. Constructed of durable powder coated steel for long life and great looks.
- 391123001
- 391123001
- 804314281793
- Outland Automotive
- New
Shipping Information
- Item Requires Shipping
- 1.0 lbs.
- W8.0000" x H4.0000" x L11.0000"
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text:
SoOfficial Model
Dani Marie PolettiHair Stylist Female Collegeville, Pennsylvania, US
Mayhem # 3170964
About MeHello! My name is Dani Marie! I am a hairstylist working in Collegeville Pa. I have a undeniable passion for all things hair and beauty. I am working towards being a color specialist as well and specializing in short haircuts . My niche is also in styling, makeup, and eyebrow shaping. As of now I am mainly working in the salon but I am eager to expand my portfolio into editoral.
Please feel free to message me with any questions about my in salon hair services, as I will offer a Model Mayhem discount to anyone for their first visit.
Outside the salon for shoots, I am willing to work for TF
Have you worked with Dani Marie Poletti? Add Credits for Dani Marie Poletti! >>
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text:
Tuesday 16 April 1996
It was Tuesday, under the sign of Aries. The US president was Bill Clinton (Democratic).
In that special week of April people in US were listening to Because You Loved Me by Celine Dion.
In UK Return Of The Mack by Mark Morrison was in the top 5 hits.
James and the Giant Peach, directed by Henry Selick, was one of the most viewed movies released in 1996
while The Green Mile, Part 1: The Two Dead Girls by Stephen King was one of the best selling books.
On TV people were watching Cañaveral de pasiones.
If you liked videogames you were probably playing 2Xtreme or Big Red Racing.
But much more happened that day: find out below..
You can also have a look at the whole 1996 or at April 16 across the years.
Old Newspapers
Have a look at the old newspapers from 16 April 1996 and get them!
Which were the most popular movies released in the last months ?
TV Series
Which were the most popular TV series released in the last months ?
Find out your future
Get a FREE Numerology report based on the digits of 16 April 1996!
Wish him/her..
Historical Events
Which were the important events of 16 April 1996 ?
- Saint Paternus
- Saint Bernadette
- Blessed HervT of Tours
- Saint Turibius
- Saint Fructuosus
- Saint Drogo.
- Saint Benedict Joseph Labre
- Mawlid in Shi'a Islam (2006).
- Birthday of the Danish sovereign, Queen Margrethe, celebrated in Greenland.
- Saints Martial, Urban, Eventius, Caecilian, Julia, and their companions, martyrs of 304
Famous Birthdays:See famous people born on 16 April
Famous Deaths:
- Stavros Spyros Niarchos: Greek shipowner, dies at age 86.
- Raymond Earl Hill: saxophonist, dies at age 62.
- Stavros Niarchos: Greek entrepreneur who was an international shipping magnate, as well as an art collector.
- Madeleine Bourdouxhe: Writer, dies at 89.
- Lucille Bremer: dancer/actress (Ziegfeld Follies), dies at age 73.
Music Charts
Which were the top hits in that special week of April 1996 ?
Top #10 songs in the USA
- Celine Dion - Because You Loved Me
- Mariah Carey - Always Be My Baby
- Tony Rich Project - Nobody Knows
- Alanis Morissette - Ironic
- R. Kelly - Down Low (Nobody Has To Know)
- 1,2,3,4 (Sumptin' New) - Coolio
- Brandy - Sittin' Up In My Room
- Busta Rhymes - Woo-Hah! Got You All In Check
- SWV - You're The One
- Whitney Houston - Count On Me
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...and if 16 April 1996 was your Birth Date then Join our Birthday Club!
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Genealogy: find out your family heritage
Magazine Covers
What news were making the headlines those days in April 1996?
- Time: Unabomber Ted Kaczynski in custody. Inset: Harvard yearbook picture from Sygma.
- SportsIllustrated: Sports Illustrated: no.9530
- EW: Entertainment Weekly: no.322
Which were the most popular books released in the last weeks ?
Remember when
What videogames would you be playing back in the days ?


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24 tire CD, fine workmanship, beautiful patterns; 12 sheets, 1 pieces of CD / DVD are arranged in each positive and negative), special suitable for family, office, DJ
|Form Color
|Black + Silver
|1 set
|Dimensions & Weight
|5.51 in x 5.51 in x 1.57 in (14 cm x 14 cm x 4 cm)
|3.81 oz (108 g)
|Packing List
|1 x CD bag
All packages from DX.com are sent without DX logo or any information indicating DX.com. Due to package variations from suppliers, the product packaging customers receive may be different from the images displayed.
To enable volume discounts on this site, use coupon code: BULKRATE during checkout. You will see a discount applied at the bottom of the shopping cart. Competitive pricing is available. Contact us for details.
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Zeer handig zwart kunststof "autoband" model disk-map voor wel 24 schijfjes , te gebruiken in de auto of gewoon voor thuis , mooi en veilig op te bergen achter de handige rits.
looks good, funny and can really carry the cd's in a funny way. doesnt take much space.. good decoration for your room\car. a bit of a cheap nylon quality of the cd covers, may be tearable. plastic is also a bit cheap. cd... Read more
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Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
Risley Ronald R DVM practices at 1938 Dam Road, West Branch, MI 48661.
Veterinarians offer general and emergency pet care services. Some veterinarians offer 24 hour emergency services-call to confirm hours and availability.
To learn more, or to make an appointment with Risley Ronald R DVM in West Branch, MI, please call (989) 345-5363 for more information.
Be the first MLive.com user to add photos or videos of Risley Ronald R DVM
Risley Ronald R DVM can be found at Dam Rd 1938. The following is offered: Pet Supplies. The entry is present with us since Sep 10, 2010 and was last updated on Nov 14, 2013. In West Branch there are 1 other Pet Supplies. An overview can be found here.
Risley Ronald R DVM is a Veterinarians facility at 1938 Dam Road in West Branch, MI.


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Home > Product Categories > Connectors & Adapters > Video Connectors > BNC Connectors > BCP-VA3
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text:
Looks like Rihanna's Battleship film debut isn't exactly smooth sailing for the star.
Analysts expected the film to do $40 million at the box office over the weekend, reports USA TODAY's Scott Bowles. But it came in second place to Avengers with $25.4 million.
Maybe Rihanna needed better lines. As Vulture points out, the singer's performance was a master class in one-liners (the blog tracked every line of her dialogue).
Here's a sample:
"What's wrong with you, drama queen?"
"Get up, princess! Come on!"
"Shut up. Shut up."
"Oh, this gon' be sweet. He hates the man."
"You go mess with him and see what happens!"
"Kentucky Fried Chicken!"
"You look like Colonel Sanders, actually."
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text: I saw unbleached cake flour in my local north Florida supermarket this morning. Has anyone used this-I have not seen it before? For everyday home cooking, I use unbleached all purpose flour but cake flour in general is for a more refined product. Would there be a difference if you used unbleached cake flour rather than regular unbleached all purpose flour?


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Christine Nesbitt of London, Ont., continues to show she's the fastest skater among Canada's women, even on sticky ice, claiming the title of 3,000-metre champion at the 2012 Canadian Long Track single-distance championships at Calgary's Olympic Oval.
Meanwhile, Mathieu Giroux, of Pointe-aux-Trembles, Que., won his first Canadian title in the 1,500-metre race.
Nesbitt, who already has six individual World Cup medals this season, won the 3,000 at Calgary in a time of 4:07.93, beating Winnipeg's Cindy Klassen (4:09.52) and Brittany Schussler (4:10.46).
"We were all talking about how slow it was," said Nesbitt. "None of us skated particularly fast or impressive times... But I was really happy with the fight I showed at the end. I'm still learning to be a 3K skater. I haven't really put together a 3K race that shows where I am physically," said Nesbitt, who gets much more practice winning 1,000 and 1,500-metre races
Giroux won the men's 1,500 metres in 1:45.29, edging out Denny Morrison (1:45.64), of Fort St. John, B.C., by 0.35 seconds, and Regina's Lucas Makowsky (1:45.93) by 0.64 seconds.
In a separate competition for domestic ranking -- Canada Cup grouping races -- Nick Goplen, from B.C., was first (1:51.78) in the men's 1,500-metre race. Daniel Dubreuil from Quebec was second and Saskatchewan's Elliott Nelson was third. In the women's 3,000-metre race, B.C.'s Sarah Pousette took the top spot (4:31.68), followed by Ontario's Isabelle Weidemann and Noemie Fiset from Quebec.
The Canadian championships and Canada Cup #2 continue Friday. Those races will feature the men's 1,000 and 5,000 metres, and the women's 1,500 metres. On Saturday, the women will compete in the 1,000 and 5,000 metres, and the men in the 10,000 metres. On Sunday, the final day, the men and women will race two 500 metres and the mass start.
Report Typo/Error


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Visual Comfort Lighting E.F. Chapman Pimlico 1 Light Swing-Arm Sconce
$ 567.00
This 1 light Swing-Arm Wall Light from the E.F. Chapman Pimlico collection by Visual Comfort Lighting will enhance your home with a perfect mix of form and function. The features include a Antique Nickel finish applied by experts. This item qualifies for free shipping! Check the right-hand bar or call our dedicated Sales Team for similar items and additional options not pictured.
- Collection: E.F. Chapman Pimlico
- Number of Lights: 1 light
- Wattage Per Light: 60W
- Total Wattage: 60W
- Shade: Linen
- Bulb Type: Hi-Lo A
- Bulbs Included: No
- Wiring: Portable
- Dimensions: 25" d x 17" h
- Shade Dimensions: 7" x 12" x 9"
- Backplate/Canopy Width: 3"
- Backplate/Canopy Length: 6.5"


text:
ActiveX control that makes skins in VB forms with new titlebar and rounded look
Use this security logo:
Buy vbSkinner Pro for $70.00.
Tutorial not found. Let know us about any useful video tutorial.
controls, basic, skinning, customize, ocx, shape, components, feel, programming, skinned, active, vb6, activex, transparency, visual, forms
Bugs fixed.
The setup program or the ocx file can not be uploaded to a web site for download, but the file posted in our web site can be linked from another web site.
The setup program may be included in any CD compilation.
If you have a licence key, it can not be included in a CD-ROM compilation or posted in a web site, or other actions like these as described in the End User agreement.
This software is provided as is, without no type of warranty about its use, the operations that it perform and the possible existence of bugs not detected during its development. Use it assuming the whole responsibility.
This software is protected by the international laws of author's copyright. The distribution of its copy of evaluation is allowed, but the REGISTRATION DATA with the LICENSE KEY are exclusive property of the licensed user, being expressly prohibited its diffusion, transference, lending, etc.
In the cases that vbSkinner should be used with more than one developer in more than one PC additional license keys must be purchased for every developer.
The use of software assumes the responsibilities and limitations established in this licensing agreement.
Limitations of the copy without license:
vbSkinner Pro works without limitations but only in the development environment of Visual Basic, once the program is compiled is necessary a license key for its normal use.


text:
Admire the most beautiful places of Vaglio Basilicata and add them to your basket. YAMGU will create your trip considering distances, opening hours and weather.
The archaeological area contains an important federal sanctuary Lucan, situated in a wooded area near a spring and dedicated to Mefite, Goddess osca marked by power to heal with water. In the 2nd century BCE the sacred area is undergoing a massive restructuring linked to the Roman presence in the area, and remains active until the first half of the 1st century a.d. The numerous inscriptions discovered in it which have oscan language writing document a strong community identity lucana.
The sacred complex is composed of a courtyard, a large paved area for large flagstones of irregular limestone,
Archaeological area situated on a natural Acropolis s.l.m 1000. to the Basento Valley. The site is related to an indigenous settlement attended from the 8th century. BC
Between the late 6th and early 5th centuries BC the Organization of urban space is divided along a main street, which runs in East-West direction, paved with large flagstones and intersected by perpendicular streets, on which overlook the houses.
The coeval returned necropolis funerary figurative ceramics of Greek type, gold and amber jewelry and armor of Greek type. These are the burials of those Basileis (King), remembered by a
The preparation of the rural civilization Museum collects objects, about 600, spanning a period between 1850 and 1960. They have been the subject of active research among the local population and also spontaneous gift many citizens. Objects, once restored by the same organizers, were catalogued and exposed with its inventory card following the Assembly policy for activities. On each card was the name in Italian and dialect language vagliese, or year of use, the donor and the name of the user, as well as a brief description of the object
Visiting the Museum you can find out with an overview of t
In line with the most advanced conception of territorial Museum, pointing to the knowledge and development of local resources, in 2006 to Screen is opened the "Museum of the ancient people of Lucania". The show offers for educational virtual reconstructions, life-size and major archaeological contexts with faithful reproductions of materials that increase the accessibility and usability of the cultural heritage. Fitting in the regional Museum system through a dense network of thematic connections, the Screening complements the path in archaeological sites of Serra and Rossano, according to wha


text:
3 Min Read
* Wheat up more than 6 pct this week, biggest such rise in a year * Corn, soybeans gain for 2nd day on short-covering bounce (Adds comment, detail) By Naveen Thukral SINGAPORE, Oct 14 (Reuters) - Chicago wheat climbed for a second session on Friday, with the market poised for its biggest weekly gain in almost a year as strong demand prompted investors to cover short positions. Corn rose nearly 1 percent, adding to Thursday's 3.7-percent rally, while soybeans were little changed after advancing more than 1 percent the last session with gains capped by expectations of record U.S. production. Chicago Board of Trade most-active wheat contract has climbed 6.2 percent this week, the most since the end of October last year. Corn gained 3.7 percent, the biggest weekly climb in more than a month and soybeans were largely unchanged for the week. "There is ample supply of wheat, but the quality of milling wheat is an issue," said Rajesh Singla, head of agriculture research at Societe Generale. "We have problems with the crop in France, Australia is facing adverse weather and the U.S. has some dryness. U.S. wheat market has been oversold and it is trading below the cost of production." Commodity funds were net buyers of CBOT corn, wheat and soybean futures on Thursday, traders said. The funds have held a historically large net short position in CBOT wheat as of Oct. 4, leaving the market vulnerable to short-covering rallies. On Friday, wheat rose 0.7 percent to $4.19 a bushel by 0257 GMT, corn added 0.9 percent to $3.52-1/2 a bushel and soybeans were unmoved at $9.56-1/4 a bushel. Saudi Arabia and Egypt both announced plans to buy wheat. Algeria announced a purchase tender earlier this week, and Syria struck a deal to purchase 1 million tonnes of Russian wheat. Soybean prices face resistance from a record-large U.S. crop. The U.S. Department of Agriculture on Wednesday raised its forecast for U.S. soybean production to 4.269 billion bushels. Grains prices at 0257 GMT Contract Last Change Pct chg Two-day chg MA 30 RSI CBOT wheat 419.00 3.00 +0.72% +2.89% 402.21 68 CBOT corn 352.50 3.00 +0.86% +2.03% 336.98 67 CBOT soy 956.25 0.00 +0.00% +0.21% 959.73 51 CBOT rice 10.21 -$0.02 -0.20% -1.78% $9.83 63 WTI crude 50.56 $0.12 +0.24% +0.76% $46.92 64 Currencies Euro/dlr $1.104 -$0.016 -1.39% -0.95% USD/AUD 0.7600 0.003 +0.33% +0.24% Most active contracts Wheat, corn and soy US cents/bushel. Rice: USD per hundredweight RSI 14, exponential (Reporting by Naveen Thukral; Editing by Joseph Radford)


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text:
- availability of sheet glass
- номенклатура (по толщине, размерам и массе) листового стекла
Англо-русский строительный словарь. 2013.
Англо-русский строительный словарь. 2013.
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text:
Well here we are at the conclusion Week 3 for the Buccaneers as they now sit at 1-2 after losing a very winnable game in Houston and the knee-jerk reaction once again from many is to fire head coach Lovie Smith. But would that be a good thing?
Social media is sounding off. Fans are convinced that Lovie does not belong on the sidelines. While that holds true to an extent, it's actually not as smart as many think.
The Bucs were destroyed in Week 1 at home against the Titans. That left a bad taste in the mouths of everyone within and around the team -- fans, coaches, etc. Week 2, Tampa Bay was victorious against the lowly New Orleans Saints. Although they shot themselves in the foot often, the Buccaneers took advantage and that's the first step for becoming a good football team.
However, they took a step back on Sunday. The lack of execution was evident. From dropped passes to missing tackles in the run game, you name it, the Bucs featured it. Some will say that is the fault of the player. Others blame that on the staff not preparing the team. Another crowd just blames Lovie Smith.
This may sound crazy, but Lovie Smith wasn't on the field Sunday. Lavonte David, Gerald McCoy, Logan Mankins -- those guys were on the field. Those guys didn't do enough to help the team win. Mike Evans, at some point has to stop asking for a flag after nearly every incompletion. Especially when he is the one getting flagged and dropping passes.
Alfred Blue was a stud. Ryan Mallett was efficient. Things like that only happens to the Bucs, no matter who the coach is.
Removing Smith from the picture will only result in more turnover, more time to get back to winning again. Yes I know, the argument of continuing to lose under Lovie Smith is also valid. But you can't continue to flip coaches.
Lots of these players were brought in by Smith. But it's time to ask the players to play. Something they hasn't done in years -- under Raheem Morris, under Greg Schiano, and not under Lovie.
This is a collective failure. Not a failure due to one name.


text: Contents[show] Hisagomaru Anime Images Profile Images HisagomaruHisagomaru's Physical ManifestationAdd an image to this galleryBeast Swords arc (anime only) Hisagomaru tries to return to sword form.Hisagomaru appears before the group while wearing a cloak.Hōzukimaru and Nanao chase Hisagomaru.Hisagomaru crashes into Hanatarō.Hōzukimaru demands to know what Hisagomaru did with Ikkaku.Hisagomaru looks at Hanatarō's Zanpakutō.Hisagomaru extends a large cannon from its chest.Hanatarō and the others with his Zanpakutō spirit.Hisagomaru falls on his back.Hisagomaru opens a crate as Hanatarō's chickens fly out.Hanatarō and Hisagomaru leap toward each other.Hisagomaru sits in front of a fire.Add an image to this galleryHisagomaru Video Clips Hisagomaru uses the healing powers of his Zanpakutō.Hisagomaru uses Akeiro Hisagomaru.Add an image to this gallery Retrieved from "http://bleach.wikia.com/wiki/Hisagomaru_(Zanpakut%C5%8D_spirit)/Image_Gallery?oldid=473638" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Images