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Morning Radar: Three things we're talking about this morning
Holiday un-cheer: University of Texas' cancer centre has put out a release cautioning holiday partiers to think about the risks of the big C when imbibing. "Research shows that drinking even a small amount of alcohol increases your chances of developing cancer, including oral cancer, breast cancer and liver cancer," according to Clare McKindley, the clinical dietitian in MD Anderson's Cancer Prevention Center.
While the centre does mention that drinking small amounts of alcohol may protect the body against coronary heart disease and type 2 diabetes - and some evidence even suggests that red wine may help prevent cancer - it's as if they know we can't stop at "small amounts."
So, they include tips, such as stick to the recommended serving size (a drink really is 1.5 ounces and you should have no more than two a day), select low-calorie options (no eggnog), and stay away from 100-proof liquor (what were you going to make with that, anyway?).
One Canadian researcher would add one more: skip the caffeine-enchanced tipples.
A Dalhousie University study suggests students who drink alcohol with energy drinks nearly double the amount of alcohol they consume. The 72 students at the Halifax university who were interviewed for the study said they consumed more than eight drinks on occasions they mixed alcohol and energy drinks, reports CBC.ca. Pass the sparkling water.
Anxiety ground zero: A University of Alberta neuroscientist has discovered what tips off anxiety in the brain. Yes, it could be your office Christmas party or the spectre of spending the holidays with just too much family, but researcher William Colmers has zeroed in on the exact brain function that makes it happen.
Dr. Colmers uncovered a "previously undiscovered mechanism in the brain that controls anxiety," according to this Montreal Gazette piece.
That mechanism affects neurons in the pea-sized part of the brain that assesses risk and reward, making those neurons more or less excitable and therefore more or less likely to send out anxious messages, the paper reports. While it may lead to targeted drug therapy, this discovery can't do anything about your skyrocketing stress this week, sorry.
Soccer blaze: And you thought the soccer moms in your neighbourhood were remarkable. But even the most intense sports parent has nothing on these Polish soccer fans, who take it to the next level.
They're cheering - and flaming - on the popular Polish squad Lech Poznan, according to Gawker. The players, they note, are 11-years-old.Report Typo/Error


text: The Drak'Tharon Overlook is a large area just pass the Raptor Pens in Drak'Tharon Keep. Essentially just the top of Drak'Tharon Keep, this overlook allows one to see across the lands in all directions. The Prophet Tharon'ja can be found on the top platform here, as well as Drakuru's Brazier.

subset: commoncrawl


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|Message view
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|woodstockrich <RichPat...@shaw.ca>
|Re: HTML/JS works in Demo but not under JBOSS 7.1 / Eclipse
|Fri, 27 Apr 2012 17:25:04 GMT
Very close. I changed the context-param org.apache.activemq.brokerURL value to tcp://localhost:61616 in my web.xml and also in broker-config.xml in the rar file I made sure that transportConnector uri="tcp://localhost:61616" Cleaned and restarted my JBOSS. Deployed my web application. Ran a separate Java application which pushes out data on a topic and listens for messages from the client. Invoked the URL to my webpage, and voila, my Java application got the message sent to it from my web application. However, my web client is not getting any messages back. Looking at the Web traffic via Fiddler, it makes 3 calls to amq: destination=topic://T&message=stocks&type=listen destination=queue://B&message=login&type=listen destination=queue://A&message=JMSTYPE:LOGIN_REQUEST SENT_FROM:DISPBOARD_JS_EMBED-APR271003&type=send However, it is not going into the behavior I saw before which is the continuous looping of /amq/timeout=... Suggestions please. -- View this message in context: http://activemq.2283324.n4.nabble.com/HTML-JS-works-in-Demo-but-not-under-JBOSS-7-1-Eclipse-tp4584105p4593271.html Sent from the ActiveMQ - User mailing list archive at Nabble.com.
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Tire/Wheel advice & opinions please Irok vs. MTR
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PASS Support (Post Abortion Stress Syndrome)www.afterabortion.com
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Understanding Abortion's Aftermath
by Grace Kern, Director of Word of Hope
Since 1973, there have been more than 58 million abortions in the United States.
LFL's Word of Hope receives around 250 calls each month from people across the country hurting because of a past abortion. Many say the experience has devastated their lives, causing long-lasting emotional, psychological, and spiritual trauma.
Counselors and pastors increasingly attest evidence of post-abortion trauma. Abortion aftermath is largely ignored by most in the general public. Groups organized to protect abortion on demand claim that abortion is no different than any surgical procedure and that the pro-life groups are fabricating the existence of post-abortion suffering. Consequently, many women think that their grief reactions are somehow abnormal and believe that they are the only one experiencing these feelings and that there is nowhere for them to turn.
Who are these women? They are your aunts, your mothers, your cousins, your neighbors, your co-workers, and your church friends. These women come from all different age groups, races, and religions. They are single women, married women, and divorced women. So where are all these women and why aren't they talking about it? The fear of negative reaction from family and friends keeps most women silent.
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The Washington Trust for Historic Preservation wants to provide a rewarding experience to our website visitors. Part of achieving that goal involves gathering information that helps ensure our site meets your needs. We respect your privacy, and this privacy statement provides important information about how we gather and protect the personal information of our website visitors.
Personal Information
You are not required to give us personal information when you visit our website. We may collect personally identifiable information (name, email address, physical address, and other unique identifiers) only if you specifically and knowingly provide it. If we collect personally identifying information, we will use it only for such purposes as described at the point of collection.
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As with most websites, we gather routine statistical information as visitors browse through our site. We use these statistics to make our site more useful to visitors and to improve the services we offer. We may share aggregate information—which does not contain personal information—with other organizations.
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Visitors may pay membership fees or make donations safely via our online contribution service provided by the National Trust for Historic Preservation. In addition, visitors may register for events via our online ticket company, Brown Paper Tickets. The security of your financial information (credit card type, number, and expiration date) are a primary concern of the Washington Trust. The credit card services on the Washington Trust's website are provided via a link to secure sites that protect the security of your information during transmission by using Secure Sockets Layer (SSL) encryption. Through these websites, credit card information is collected confidentially and securely and is used only for your intended purpose.
The Washington Trust for Historic Preservation will not knowingly collect personally identifiable information from children under the age of 13 without first gaining consent from a parent or guardian. If your child sends us an e-mail request for information (such as information for a school project), we will respond using the e-mail provided, but we will not use it for future communications without the consent of the parent or guardian.
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FDA Gives Avastin for Breast Cancer Thumbs DownNov 21, 2011 | Parker Waichman LLP
The U.S. Food & Drug Administration (FDA) announced its final decision to revoke Avastin's approval for breast cancer. In announcing its decision, the FDA cited Avastin's serious side effects, and a lack of evidence showing Avastin will either help them victims of metastatic breast cancer live longer or improve their quality of life.
Avastin will remain on the market as a treatment for certain types of colon, lung, kidney and brain cancer (glioblastoma multiforme).
"This was a difficult decision. FDA recognizes how hard it is for patients and their families to cope with metastatic breast cancer and how great a need there is for more effective treatments. But patients must have confidence that the drugs they take are both safe and effective for their intended use," Dr. Margaret Hamburg, FDA commissioner, said in a statement.
Because Avastin will remain on the market, doctors could continue to use it off-label as a treatment for metastatic breast cancer. Whether insurers will still to cover the $80,000+/year bill for the drug remains to be seen. Medicare, however, which covers many women with breast cancer, has already said it would continue to cover the cost regardless of the FDA's action.
Avastin was approved for metastatic breast cancer in February 2008 under the FDA's accelerated approval program. But two studies conducted by the drug's maker, Genentech, later showed only a small effect on tumor growth without evidence that patients lived any longer or had a better quality of life compared to taking standard chemotherapy alone. Serious complications from Avastin include bleeding and hemorrhage; the development of perforations in the intestines and other areas of the body; and heart attack or heart failure.
According to the Associated Press, breast cancer organization Susan G. Komen for the Cure said it respected the FDA decision and that it was time for researchers to focus on finding so-called biomarkers that would tell which drug is right for which patient.


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Last week, Whoopi Goldberg spoke at the Child Mind Institute 13th Annual Adam Katz Memorial Conversation, where she detailed her life growing up with dyslexia.
During the conversation, NY Mag reports, Goldberg revealed that to teach her daughter about body image she once chewed off a Barbie doll's arms.
RELATED: Whoopi Goldberg Launches Medical-Marijuana for Menstrual Cramps
"I wanted to explain to her that she didn't have to look like that," said The View co-host.
She added:
RELATED: Watch Whoopi Goldberg and Michael Strahan Perform Dramatic Readings of Kanye West's Tweets
"What I realized is you can talk to girls all you want to about what they shouldn't be feeling ... They know who they are. But, we have been taught — and I think women are still led down this idea — that there is something better than you ... If we saw more portly women on magazines, we would think they're beautiful, but we don't. We don't see very portly women. We see women talking about, Oh yes, I gained all this weight for a movie part, but then I lost it all again. Or somebody has a baby on Wednesday and they weighed you know, 116 pounds, and then on Friday they're back to, you know, 98. And it's because this idea of what we're supposed to be hasn't changed in hundreds of years. And so fighting all of that was my biggest issue."
The annual conversation aims to raise awareness about mental health and learning disorders.
RELATED: Whoopi Goldberg Slams Ben Carson for Endorsing Donald Trump
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Dramatic Start to Amp'd Mobile World Supercross GP as Reed Wins Davalos Dominates Supercross Lites Class TORONTO (December 2, 2006) -- Yamaha's Chad Reed won the opening race of the Amp'd Mobile World Supercross GP at Rogers Centre in front...
Dramatic Start to Amp'd Mobile World Supercross GP as Reed Wins
Davalos Dominates Supercross Lites Class
TORONTO (December 2, 2006) -- Yamaha's Chad Reed won the opening race of the Amp'd Mobile World Supercross GP at Rogers Centre in front of 33,412. KTM's Martin Davalos led all 15 laps in winning the Amp'd Mobile Supercross Lites main event.
Honda's Nick Wey grabbed the $1,500 Progressive Direct Holeshot Award with Suzuki's Ricky Carmichael and Reed in tow. Stewart started in 10th place. Throughout the first lap Reed and Carmichael traded positions, but the start of lap two, Carmichael hit a tuff block and crashed. Stewart moved into second place and put the charge on Reed.
Reed and Stewart swapped positions several times before they came together before the finish line jump on lap 17. Stewart went off the track and upon re-entering Honda's Travis Preston collided with Stewart, causing both of them to crash. Reed went on to pick up the win. Carmichael battled back to finish second and Stewart finished third.
"I came here with an open mind, and here I am on the top of the podium," said Reed. "The first time James passed me, he almost knocked me over and that fired me up. It is a shame that he crashed, but that happens. Every time I made a pass, the crowd was so loud.
"I know I am the guy who is in the best shape out there. It would have been nice to have all three of us battling up there tonight. There are a lot of people that helped me put this team together, and I am very appreciative. I am excited to get a win for the team."
Reed, who won the 2003 World Supercross GP championship, heads to Vancouver's B.C. Place Stadium with a three-point lead over Carmichael in the season standings. Stewart sits in third with 20 points.
After earning the $1,000 Progressive Direct Holeshot Award, Davalos was able to extend a comfortable lead over Yamaha's Broc Tickle in the early part of the race. Davalos kept Tickle in check and went on to win his first Amp'd Mobile Supercross Lites main event. KTM's Zach Osborne was third.
"I got a great jump off the gate and rode my own race," said Davalos. "I wanted to put 15 laps together on the new bike. Zach Osborne is a training partner of mine, and I am happy."
Tonight's race will air on SPEED Sunday, December 3, at 6:00 p.m. EST. The Supercross Lites race will air on Saturday, December 9, at 6:00 p.m. EST.
Amp'd Mobile World Supercross GP Event Results, Toronto
1. Chad Reed, Yamaha
2. Ricky Carmichael, Suzuki
3. James Stewart, Kawasaki
4. Tim Ferry, Kawasaki
5. David Vuillemin, Honda
6. Nick Wey, Honda
7. Ryan Clark, Honda
8. Paul Carpenter, Kawasaki
9. Heath Voss, Honda
10. Jeff Gibson, Kawasaki
Amp'd Mobile World Supercross GP Season Standings
1. Chad Reed, Yamaha, 25
2. Ricky Carmichael, Suzuki, 22
3. James Stewart, Kawasaki, 20
4. Tim Ferry, Kawasaki, 18
5. David Vuillemin, Honda, 16
6. Nick Wey, Honda, 15
7. Ryan Clark, Honda, 14
8. Paul Carpenter, Kawasaki, 13
9. Heath Voss, Honda, 12 10. Jeff Gibson, Kawasaki, 11
Amp'd Mobile Supercross Lites
1. Martin Davalos, KTM
2. Broc Tickle, Yamaha
3. Zach Osborne, KTM
4. Michael Willard, KTM
5. Troy Adams, Suzuki
6. Tyler Medaglia, Suzuki
7. William Ainsworth, Kawasaki
8. Robert Marshall, Kawasaki
9. Benjamin Ritter, Kawasaki
10. Logan Martin, Honda
KTM Junior Supercross Challenge
1. Stephane Sum, KTM
2. Nathan Cryer, KTM
3. Christopher Fortie, KTM
4. Tommy Cloutier, KTM
5. Jake Pym, KTM
6. Jean Christophe, KTM
7. Timothy Carr, KTM
8. Dominick Allen, KTM
9. Alyson Bilodeau, KTM
10. Jake Delazzari, KTM
11. Calvin Barnhart, KTM
12. Joshua Roney, KTM
13. Phillippe, Perron, KTM
14. Tommy Purdon, KTM
15. Cody Watson, KTM
-credit: www.supercrossonline.com


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Question: Logistics Consultants Inc LCI provides various logistics analysis services to
Logistics Consultants Inc. (LCI) provides various logistics analysis services to other firms, including facility location decisions. It has just completed a project for a major customer, but on the eve of its presentation it discovered a computer malfunction had partially deleted some of its data. One file that was impacted contains the final factor rating results. Following are the partial results it was able to recover. As you'll notice, some ratings are missing.
If you were the project manager for LCI, what would you do given that you are missing some crucial data?
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If you were the project manager for LCI, what would you do given that you are missing some crucial data?
View Solution:
Answer to relevant QuestionsBindley Corporation has a one- year contract to supply motors for all washing machines produced by Rinso Ltd. Rinso manufactures the washers at four locations around the country: New York, Fort Worth, San Diego, and ...Santa Cruz Bottling is a manufacturer of organic soft drinks on the coast of central California. Its products are enjoying a growing reputation and increased demand through-out the American Southwest. Because of the high ...Describe how outsourcing works. Why would a firm want to outsource?1. Evaluate the current China/Taiwan logistics costs. Assume a current total volume of 190,000 CBM and the 89% is shipped direct from the supplier plants in containers. Use the data from the case and assume that the ...Here are the data for the past 21 months for actual sales of a particular product:.:.Develop a forecast for the fourth quarter using a three- quarter, weighted moving average. Weight the most recent quarter .5, the second ...
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Kenneth says that Iowa State took notice of him just a few months ago.
"They started looking at me around early June," he said. "Coach Bray told me that they saw my highlight tape and that they could really work with me at Iowa State. He said they really like my speed and coverage skills."
While the 5-foot-11, 165-pound cornerback is very interested in Iowa State he made it clear that at the moment he would rather stay closer to home.
"Coach Bray wants me to come up for a game and I definitely want to go there for a visit," he said. "If I get a chance to stay closer to home I probably would because I really want to be closer to Texas but I would definitely like to check them out."
Kenneth had two interceptions last season and says that he would like to have his decision made for his senior year begins.
"I will very likely make my final decision before my season begins," said Lynn. "I want to major in sports management and I would also like to redshirt my first year so that I could bigger."


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It's the most valuable real estate in the whole damn store.
It's just a few square feet by the cash registers, but every single customer is eventually funneled through the checkout lanes, and its merchandise is reachable by even the littlest of shoppers. Candy has always been a top seller for supermarkets, but in recent years it's moved to the front of the store at specialty retailers like Old Navy, Bed Bath and Beyond, Babies R Us, and Sports Authority.
Most shoppers assiduously avoid the candy aisle.
Just 25% will even go there, and when they do, they linger for fewer than 30 seconds. But good intentions and self-restraint are no match for the extended captivity of the checkout lanes where 58% of shoppers buy candy at least once a month. We're not talking about the chewing gum and mints that 63% pick up on a regular basis, but real candy like Kit Kat bars and Twizzlers and M&Ms.
Cigarettes are out; candy is in.
Retailers are going tobacco-free, following the lead of stores like Target and CVS, and where they're not, municipal governments are imposing their own sales bans. Stores have leapt to fill the void left by cigarettes with expanded offerings of soda, chips, and especially candy. In the process we've traded one threat to public health for another.
The New England Journal of Medicine addresses the insidious nature of sugar consumption in the article Candy at the Cash Register — A Risk Factor for Obesity and Chronic Disease. The authors takes retailers to task for the way they harness sophisticated marketing techniques to deliberately bypass our cognitive controls and steer us toward unhealthy impulse purchases. The authors contend that it's not the candy itself, but its placement at cash registers that creates the risk factor, and argue that that moving candy to other store locations should be mandated as a service to public health. They say it's just like safety requirements for window guards or balcony railings—we know it's dangerous to go right to the edge, but sometimes we wander a little too close and need to be protected from our own limited capacities.


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Forum Index > Saints Row: The Third > Season Pass Help
Posted by OccupySkyrim on 2012-02-14 15:01:33
Hello everyone! I need help with my season pass for saints row TT. You see, I have bought one, but only got Nyte Blade pack, how do I get the Professor Genki's Island Pack? can anyone help me, for I am very worried that I have wasted 20 bucks, and one of my friends said I can download it seperatley for free. I have a 360 btw, so please help me!
OccupySkyrim, 2012-02-14T15:01:33Z
- You have to buy the DLC as well as the pass. The season pass just means you pay less for it. However, you apparently have to buy the DLC through the game, NOT the PlayStation store (or whatever the XBox equivalent is called), otherwise you won't get the discount.
- Leigh Burne, 2012-02-14T15:51:00Z
- Wrong. The Season Pass isn't a discount, it's prepaid access to all three DLC packs. If I'm reading this right, there's an in-game store; you go to that and select to download the pack, rather than going to the XBox Live store itself.
- The confusion arises from the pack saying it offers the content at a discounted rate over individual purchases; what this means is you save money compared to buying them seperately, not that you still have to buy them but having the season pass gives you a discount.
- Evil Tim, 2012-02-14T16:26:47Z (last edited: Evil Tim, 2012-02-14T16:31:22Z)
- From the Saints Row website:
- "Strapped for cash? Strap it on with the Saints Row Season Pass in order to save money on three future Saints Row: The Third DLC Mission Packs and the Nyte Blayde DLC pack. The Season Pass is a special program to ensure you get the three upcoming mission packs and the Nyte Blayde pack for a single, one-time recommended cost of $19.99, for a total anticipated savings of more than 15 percent if you purchased each DLC pack separately."
- Leigh Burne, 2012-02-14T16:30:08Z
- Exactly. It's a one-time payment for all the DLC, like I just said.
- Evil Tim, 2012-02-14T16:32:26Z
- I'm pretty sure you automatically get it when the new DLCs are released. That's what it says on the wiki at least. I'm pretty sure when it comes out you can just go and download it for free. I'm not sure, I don't have a 360, but that's probably how it works for the PS3, so I'm guessing it's the same concept for both.
- Ishimura Elite, 2012-02-14T19:07:00Z
- I'll be checing in on this page again soon. I can't access my DLC with the season pass either,
- it is called the Genki bowl pack. it is a complete expantion pack with vehicles, homies and new activities relating to the professor Genki.
- you also can't use your season pass for it, you have to buy it seperatly
- Wrong. All 3 major DLC packs are included in the Season Pass.


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Premiere Pro 2.0: Clip Notes, The Editor's FriendTutorial Series, Part 1
One of the most innovative new features in Premiere Pro 2.0 is its Clip Notes review and approval routine. It allows you to export a timeline for approval, where the video is compressed into a PDF file thats easily e-mailed or transported to a client or reviewer. When the reviewer receives the PDF file, all thats required is the ubiquitous Adobe Reader to watch the video and make comments. After entering comments, that file can be imported back into the timeline, resulting in markers placed on the exact frames where the reviewer made the comments. Heres Here's a tutorial showing you how to use it.
First, create a simple project in Adobe Premiere Pro with a shots lined up, maybe with a few cuts and dissolves here in there. It doesnt really matter what sort of segment you create, will just use that as a canvas upon which we will place all of our comments. Once youve done that, highlight the sequence in the project window. Take a look at the top of the interface and select the menu item entitled Sequence, and then click on Export for Clip Notes (see graphic below).
|Click Sequence, then Export for Clip Notes
Heres where youll see a dialog box with the heading Export for Clip Notes (see graphic below).
Lets take a look at a couple of possibilities inside of this dialog box. If you open that first drop down list, labeled Format, you can see that youll be able to export the media as either Windows Media or QuickTime. For this exercise well stay with the default Windows Media. Next, in the Range area, you can choose to export either the entire sequence or the work area. In this case go ahead and choose the default, work area. Right under that you have a choice of different encoding presets, and lets use the Low Quality Preset for the purposes of this tutorial, because it will provide the fastest export.
Underneath that group of lists, youll see settings where you decide how this file will be compressed into a more portable PDF document. There youre offered video options, where the most common choice would be to embed the video into a PDF file and simply email it to someone. Keep in mind, if you have a really long sequence and you want to do a high-quality encode, youll end up with a gigantic file, and it will take you a long time to render that. Another option here is that you can select Stream Video, and then if you switch over to the FTP (file transfer protocol) settings tab you can choose a destination to which youd like to send a particular file via FTP. This way, you can place the file on a server somewhere, and then the e-mail the small PDF file to your user or reviewer. If thats your choice, the PDF will be automatically be generated with a link to that file?so the user can open the PDF file, and the video will be served from the destination to which you pointed. But for this tutorial, just set the video options to Embed Video. Youll see under that a PDF password field where you can enter a password and confirm it, in case youre sending confidential information that youd like password protected. Go ahead and type in a password there, and note that its case sensitive.
Next, take a look at the instructions field. This is the default text that will appear in a dialogue when the user opens this PDF that youve sent. It greets whoever opens the file on the other side, and analysis that this file uses PDF technology that makes it possible to review a movie and add comments. Customize this however you wish.
Underneath that, you can enter your e-mail address in the Return Comment To field, and thats allows the comments to conveniently be sent to automatically back to you. Lets just leave that blank for now. Click the OK button, and then save this file to the desktop, which will be the easiest place to find it later. Give it a file name that means something to you and then we will move along to the next part. When you click Save youll see that Premiere Pro begins rendering your file into the PDF format. Depending on the size of your project and speed of your computer, it might take a while, about a minute per 15 seconds of video on a fairly fast computer (see graphic below).
Once thats done with its rendering, that render thermometer will disappear. At this point, minimize Premiere Pro, and on your desktop, locate the review PDF that you just generated. Youll need Adobe Reader in order to see this file, so if you dont have that on your system, download it free from the Adobe web site here. Incidentally, when I first tried using Adobe reader to open one of these Clip Notes files, I got a JavaScript error. That can be easily fixed by going to the Help menu in Adobe Reader and selecting Detect and Repair. That quickly fixed the problem. Anyway, when you double-click on that PDF file we created a moment ago, it will first ask you for your password. Fill in the password that you created, and youre in.
Next, youll see that instruction dialogue and once youve clicked through that, just enter the reviewers name of your choice into the window (graphic above). Incidentally, you can import numerous comments from any number of different reviewers into Premiere Pro, so since we would like the editor to know from whom these particular comments are coming, enter your name here.
Now its time to start reviewing the comments. Click the Play button, and whenever you want to enter a comment, click Pause. Youll see that the time code of that particular frame has been entered in the entry field. Next to the blinking cursor, enter your comment.
After that, when you click Play again, your comment is saved automatically. After youve entered a few comments, drop down the ?Go To box at the bottom of that window and take a look at what youve entered so far.
Related Keywords:new features, Adobe Premiere Pro 2.0, Clip Notes, review and approval, PDF file, Adobe Reader, tutorial, how to


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The Hennessey Expedition Asym Hammock is more than just a hammock, it is a complete ultralight shelter and hammock rolled into one. The Expedition Asym is designed for people under 110kg and under 6ft tall . If you need the bigger one then look at the Explorer Asym. The Expedition Asym Hammock has an asymmetrical shape, providing instant comfort over rocky, wet or sloping terrain as well as protection from the sun, wind, rain and insects. The Expedition Asym is shaped to keep you back straight and level when lying down. First is the classic double flap base set up - enter through the bottom of the hammock and it closes automatically once you're inside giving an insect free and water-proof sleeping environment. Second is the more compact and lower profile zipped side opening. Easier to get into but harder to keep the bugs out as you zip it shut. The weights and pack size are the same for both so which you choose is down to personal preference.
All Hennessy Hammocks come complete with attached mosquito netting, detachable rain fly, support ropes, and stuff sack with set up instructions on back. Hennessy Hammock also provides complimentary standard 42" long "Tree Hugger" webbing straps to protect the bark of trees. Because these accessory webbing straps are complimentary, they are weighed separately from the hammock.


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lokapur Overview
You can reach lokapur from Bangalore by road in about using a cab upon driving using a cab.
lokapur is a small village situated in the state of karnataka, india. located in the mudhol taluka, bagalkot district the village belongs to belagaum division. kannada and konkani are the widely spoken languages in this region however telgu and english are used too. there is no railway station in close proximity to the village however there is a major railway station in dharwar which is located 100 km away. road connectivity from mudhol to lokapur is good and transportation services can be availed easily. saavari cab services may be available too.the nearby airport is the sambre airport which is 92 km from the village. the climate is pleasantly warm owing to its elevated location above sea level and the village also receives a good amount of rainfall.. Book cabs in lokapur


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Job Summary
- Salary: Undisclosed
- Work Type: Permanent / Full Time
- Min. Education Level: Diploma
- Field of Study: Not Applicable
- Years of Experience: 5
- Skills: Customer Service
Job Descriptions
Job Number:JO-1306-56266
Industry:Technology/Online/Enterprise Software
Job Category/Function:Sales/Account Manager/Sales
About Our Client
Our client is a global market leader in virtualization software.
Job Description
- Identify, generate, propose and close sales opportunities
- Generate sales by cold/warm calling current and prospective customers
- Provide pricing, proposals and take orders over the phone and through email. Utilize phone, email and web tools to communicate and present company offerings.
- Work in a team environment with the channel sales organization
- Meet and/or exceed individual product and services revenue quota as set by management from time to time.
The Successful Applicant
- Great interpersonal and customer relationship building skills required
- Demonstrated ability to be proactive and take initiative to get things done
- 5+ years of quota carrying experience in inside Sales; preferably with technical or software companies
EA License No.:10C2978


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Reloop KeyFadr
- Description
- Additional Information
Reloop KeyFadr Product Description
Intuitive Possibilities
The Reloop Keyfadr is the compact and versatile DAW solution for producers and performers who use the popular Ableton Live software. Aside from its extremely compact dimensions, which make the controller fit comfortably into almost any laptop bag, the Keyfadr offers users a variety of intuitive possibilities; from beat programming while on the road, to setting-up a control station for studio projects as well as being a creative tool for a live gig.
Creative Tool
The Keyfadr is made up of 25 velocity-sensitive keys, 35 buttons, 8 faders, 16 rotary knobs and 8 endless encoders with push function, making this USB MIDI controller an indispensable creative tool.
Create Complex Compositions
A unique highlight of the Keyfadr is its ability to help create complex compositions on the fly. The internal arpeggiator can build vivid sequences with flexible quantization possibilities that give a subtle nuance to your sound, while the chord mode can punch out different combinations at the strike of just a single key. Scale mode helps you hit the right key every time by playing the correct tones matched to the chosen scale.
Keyfadr MIDI Editor
The specially-developed Reloop Keyfadr MIDI Editor allows you to customize the controller to your individual needs – whether in the studio or at live gigs. The MIDI Editor can map every single control element of the Keyfadr, which means each controller can now be uniquely suited to its owner.
Support for Ableton, Fruity Loops, Bitwig Studio & Reason
The Reloop Keyfadr now also supports Fruity Loops, Bitwig Studio and Reason.
Ableton Live Lite 9 included
Ableton Live Lite 9 comes included with Keyfadr, and the controller is ready to plug-and-play once the software is installed; getting you set-up and running in seconds, without having to make your own complicated mappings. Creating a live performance is easy with Keyfadr, as Ableton's live clip view can be set to control all major clip and navigation commands using only the Keyfadr surface.
- Combines the most useful functions for music production into one device
- Keyboard: 25 mini keys with 9 playable octaves
- Control section: 8 channel faders with endless encoders (pan), including 2 control knobs, (send/return) and three backlit function buttons (mute, solo, rec)
- Full integration and easy plug and play for Ableton Live 9
- DAW transport controls (play, stop, rec, scene up/down, play/ stop clips, overdub, set marker, marker left/right, cycle, tap, metronome)
- On board arpeggiator with multiple modes and internal/external MIDI clock
- Chord feature: Easily play the most common chords in this versatile mode
- Scale mode: Choose from four different scales and always hit the right key
- Shift button for second layer control and choose between two performance scenes
- Fully USB powered controller requires no external power source
- Support for Ableton Live, Fruity Loops and Bitwig Studio
- Free and extensive MIDI Editor for individual configurations available in the download section (Win Version)
Technical Specifications
- Number of presets: 2
- Note keys: 25 (velocity sensitive)
- Dials: 32
- Inputs/outputs: 1 mini USB port
- Capacity: < 100 mA, 5V DC via USB
- Weight: 920 g
- Dimensions: 320 x 29 x 220 mm
- Incl. Ableton Live 9 Lite (value upgrade opportunity)
Reloop KeyFadr Additional Information


text: The Board of Directors of Capstead Mortgage Corporation (NYSE: CMO) announced today that they have appointed Michelle Goolsby to serve as an independent member of the Board of Directors of Capstead Mortgage Corporation. With the appointment of Ms. Goolsby, the Board of Directors will consist of eight members, seven of whom will be independent. Remarked Andrew F. Jacobs, President and Chief Executive Officer, "I am pleased that Michelle has accepted the invitation to join our Board. She brings a diverse background of leadership skills including significant executive and board level experience that will complement the Board's background and serve our Company well over the long term. Michelle currently is a partner and investment committee member for Greenmont Capital Partners, a private equity firm, and previously served as an executive vice president of Dean Foods Company from 1998 to 2008 where she led several functions, most recently corporate development, sustainability and corporate affairs, after having served as general counsel. Previous to her tenure at Dean Foods Company, she provided legal representation for public and private real estate clients in security offerings, mergers, acquisitions, financings and divestitures." About Capstead Capstead Mortgage Corporation, formed in 1985 and based in Dallas, Texas, is a self-managed real estate investment trust for federal income tax purposes. Capstead earns income from investing in a leveraged portfolio of residential mortgage pass-through securities consisting of adjustable-rate mortgage ("ARM") securities issued and guaranteed by government-sponsored enterprises, either Fannie Mae or Freddie Mac, or by an agency of the federal government, Ginnie Mae.


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College of Arts and Sciences
Regular Departmental Events
Kaffestunde (German Coffee and Conversation) Kaffestunde is open to any WIU student, faculty, staff, and members of the Macomb community, who wish to practice their German language skills, no matter what level they are at. For more information contact Ms. Norma Suvak, 309-298-2335, firstname.lastname@example.com. There will not be Kaffestunde during the Fall 2016 semester.
See German Info/Conversation Flyer
Grupo de Español (Spanish Conversation Hour)
Please join us for Spanish Club! Every Thursday during the Fall 2016 from 3:30-4:30 pm in Morgan Hall 303 lab.For more information contact Luis Riofrio firstname.lastname@example.com or Margarita Obregon firstname.lastname@example.com.
Tertulias (Spanish Conversations) will not be during the Fall 2016 semesters. If it starts again, we will update this page.
French Coffee and Conversation are held every Wednesday, from 3:00 to 4:00 p.m. in Morgan Hall 303. For more information contact Dr. Edmund Asare, 309-298-1496, email@example.com.


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Latest Activity: Played Idle Mine (Jul 2, 2017 5:59pm)
Points needed for next level: 192 Level
I haven't been on in forever, if you know me, awesome, but if you don't just say hi. I'm not an ass so unless you're an idiot or an douche bag, I'll be nice. If you do end up talking to me, please just call me awes or ken, it's shorter and easier to type (also i don't like my name i made it almost 3 years ago so… But yeah, if you want to play with me on other games, I'm on League as Kenzorsu, and a few other games that I don't play as much.
Activity Feed
My Games


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Raindale Mill Volunteer
Cause Areas
WhenIt's flexible! We'll work with your schedule.
WhereYork Castle MuseumEye of YorkYork, North Yorkshire YO1 9RYGB
Raindale Watermill stood at the foot of the secluded Raindale Valley on the North Yorkshire moors. Originally built as a cottage, the waterwheel and mechanism were added around 1800 and like many mills in North Yorkshire it then provided for purely small scale local needs, grinding wheat into flour for human consumption, and oats and barley into meal to feed livestock. The mill became redundant in 1915 and in 1935 it was offered to York Castle Museum. The mill was carefully taken apart and reconstructed on its present site, behind the museum on the banks of the River Foss, opening to the public for the first time in 1966.
We reopened the mill to visitors in 2009 with our mill volunteers enabling visitors to access the building and learn about its history and the history of milling in the area. In 2011 we restored the waterwheel and mill mechanism to working order again and the outside area around the mill has undergone extensive landscaping and improvement. We no longer grind corn, so the mechanism operates with the grinding stones disengaged but the waterwheel turns, fed by a pond which draws water from the river.
The volunteer role involves meeting and greeting the visitors, showing them around and explaining how the mill worked, what its origins are and how it came to be at the Castle Museum etc. We have a small demonstration set of quern stones for visitors to have a go at grinding corn for themselves, as well as a small handling collection of milling and agricultural objects for visitors to use.
We provide object handling and customer care training to ensure that volunteers have access to all the information that they need to undertake the role. New volunteers shadow experienced volunteers until they are confident enough to take an active part in the mill sessions. Volunteers also have the opportunity to be trained to operate the mill mechanism if they wish. In addition, we have a bread making activity as part of the Castle Kitchen project which runs in the Castle Museum itself, and which obviously links very well to the mill project.
This is probably our most flexible project in relation to the time commitment that it requires. The mill can be opened any time from around 10:30am to 4:30pm (3.30pm during winter) 7 days a week. Once volunteers are confident with opening and closing procedures and are comfortable with the role, they can open the mill whenever they have some free time to come in; this can be anything from a couple of hours to a full day.
- Customer Service
- Agronomy
Good Match For
Requirements & Commitment
- Must be at least 18


text: he Australian Outback - 1932. Entranced by the moon, a young girl steps out of her bedroom window. When her parents check on her, she is gone. The police suggest aboriginal tracker Albert lead the search, but the father revolts - insisting - no black-fella is to set foot on my land. Instead, he gathers together as many white men as he can find, and conducts a line search across the desolate plains in search of the child. Albert watches helplessly as her every trace is stamped to dust. Sometime later, the daughter is still missing, and the mother makes a decision to take responsibility into her own hands, to reconcile the loss of her child. She goes to find Albert...Resumen IMDb.com


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KIEL – The world seems to be on the verge of another "great transformation," with changes far more profound than news-grabbing economic or geopolitical headlines about Asia's economic rise or the fires in the Middle East. The coming changes will fundamentally redefine the nature of our economic interactions – and the social dynamics that underlie them.
This is a transformation on the scale of the shift, more than 8,000 years ago, from nomadic hunter-gatherer societies to settled agricultural ones, which eventually led to the rise of cities. A similar transformation occurred in Europe in the tenth century, with the emergence of guilds – associations of skilled workers who controlled the practice of their craft in a particular town – which paved the way for the Industrial Revolution.
The particular characteristics of the impending transformation remain unclear. It may well involve revolutions in bio-, nano-, and digital technology, together with a social-networking revolution that eliminates geographical and cultural barriers. What is already clear, however, is that, like previous transformations, this one will involve a fundamental change in all of our economic relations and the social relations that support them.
Mainstream economics offers a straightforward analysis of and policy response to such a transformation. Whenever technological or other changes allow for people to be compensated for the benefits that they confer on one another (minus the costs), the price-based market system can adjust. When the changes create externalities, economic restructuring is required – say, adjustments in taxes and subsidies, regulatory shifts, or property-rights upgrading – to offset the costs and benefits for which the market cannot compensate. And when the changes give rise to particularly high levels of inequality, redistributive measures are needed.


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Project DescriptionA multi family residential project subdivides a block into two homes. In the Pavilion home the living and family pavilions are separated by a toddler friendly courtyard which seamlessly integrates into the living area with its large bi-fold doors opening onto terraced decks.
The open plan living and dining design enjoy high timber lined ceilings with a mezzanine study/cubby house above the kitchen.
Mum can watch her kids play -from her custom design lux kitchen- in the secure courtyard with its green herb wall. A juliette balcony in the Master bedroom suite overlooks the courtyard and living pavillion below.
The new dual occ home follows the contours of the sloping block in a Melbourne suburb adding value through the dual occupancy subdivision. See the video


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The 6th Annual Beerfest is presented by McQuade Distributing, Cloverdale Foods and Jerome Distributing.Guests will be able to sample up to 30 of the finest brews around and taste the treats of Cloverdale Foods. The charity receiving the funds this year is the Walk to Cure Arthritis Foundation.
Boulder Beer will be pouring Shake Chocolate Porter, Dragonhosen Imperial Oktoberfest and Pulp Fusion Blood Orange IPA!
September 10Screw City Beer Festival - Rockford, IL
September 17Newtown Beerfest - Newtown, PA


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Identification of novel mitosis regulators through data mining with human centromere/kinetochore proteins as group queries
Proteins functioning in the same biological pathway tend to be transcriptionally co-regulated or form protein-protein interactions (PPI). Multiple spatially and temporally regulated events are coordinated during mitosis to achieve faithful chromosome segregation. The molecular players participating in mitosis regulation are still being unravelled experimentally or using
An extensive literature review has led to a compilation of 196 human centromere/kinetochore proteins, all with experimental evidence supporting the subcellular localization. Sixty-four were designated as "core" centromere/kinetochore components based on peak expression and/or well-characterized functions during mitosis. By interrogating and integrating online resources, we have mined for genes/proteins that display transcriptional co-expression or PPI with the core centromere/kinetochore components. Top-ranked hubs in either co-expression or PPI network are not only enriched with known mitosis regulators, but also contain candidates whose mitotic functions are not yet established. Experimental validation found that KIAA1377 is a novel centrosomal protein that also associates with microtubules and midbody; while TRIP13 is a novel kinetochore protein and directly interacts with mitotic checkpoint silencing protein p31comet.
Transcriptional co-expression and PPI network analyses with known human centromere/kinetochore proteins as a query group help identify novel potential mitosis regulators.
Mitosis is a complicated cellular process involving extensive structural reorganizations in many subcellular compartments and a sequence of highly orchestrated events. The temporal and spatial changes in mitotic cells are tightly regulated to ensure high fidelity of genomic transmission during cell division. Mitosis is initiated by accumulation of active kinase complexes formed between mitotic cyclins (cyclin A and B in human) and master mitosis regulator CDC2 (or CDK1) at the G2/M transition
The centromeres are specialized loci on chromosomes that form primary constrictions during mitosis. There are currently 18 known human proteins constitutively associating with centromeres throughout the cell cycle
Although the knowledge of proteins localized at the centromere/kinetochore complex has increased exponentially in the past few years
As an end user interested in taking advantage of the huge amount of genomics and proteomics data in the public databases, we first conducted exhaustive literature review and compiled a list of 196 human centromere/kinetochore proteins, and selected 64 among them as "core" components with well-characterized mitotic functions. By interrogating and integrating available online resources using the 64 core proteins as a query group, we have then identified potential novel mitosis regulators among top-ranking genes/proteins that co-express or interact with the 64 core centromere/kinetochore components. Experimental validation has identified one novel kinetochore protein TRIP13 and two novel centrosomal proteins KIAA1377 and DDX39.
Results and discussion
Compiling a comprehensive list of human centromere/kinetochore proteins
Several reviews have previously summarized known centromere/kinetochore proteins in human cells
Additional file 1
Table S1. Compilation of human centromere-kinetochore proteins.
Click here for file
When compared to previous summaries, the compilation has significantly expanded the list of known human centromere/kinetochore proteins, from ~120 to 196. The list still did not include all the subunits of several well-characterized protein complexes such as the dynein-dynactin complex and the γ-tubulin ring complex, both shown to associate with the centromere/kinetochore
We compared our list of human centromere/kinetochore proteins to annotations in Gene Ontology (GO) (
Additional file 2
Table S2. Comparison of the centromere/kinetochore list compiled in this work with GO annotated centromere/kinetochore genes.
Click here for file
Genes co-expressing with 64 core centromere/kinetochore components
Transcriptional co-expression profiling has been extensively used to uncover functional gene modules involved in common biological processes
In our initial efforts, we adopted a simple rank-based method to retrieve genes that co-express with a selection of 64 "core" centromere/kinetochore components (Table
Additional file 3
Table S3. Summary table of all "top 50" genes that co-express with the 64 core centromere/kinetochore queries in Gene Sorter search.
Click here for file
The genes that appeared more than 9 times in the combined co-expression list were summarized in Table
Additional file 4
Table S4. GO enrichment analysis of genes listed in Table.
Click here for file
The genes co-expressing with 64 centromere/kinetochore components were further analysed using the CoExSearch program which accepts a group of queries to search and rank common co-expressing genes
Additional file 5
Table S5. Top-ranking co-expressing genes retrieved by CoExSearch program.
Click here for file
Additional file 6
: Table S6. Genes co-expressing with the 64 core centromere/kinetochore components in both Gene Sorter and CoExSearch searches. Highlighed genes were experimentally tested in this work.
Click here for file
Proteins interacting with 64 core centromere/kinetochore components
We then used POINeT website to obtain the "sub-network specific" PPI data with the 64 core centromere/kinetochore proteins collectively as a query group (see Methods for details)
Additional file 7
Table S7. Top-ranking genes whose encoded proteins interact with the core centromere/kinetochore components.
Click here for file
The mitotic functions of several other proteins are less well characterized but they were all reported in literature to interact with at least one of the 64 centromere/kinetochore proteins. Of interest, adapter proteins AP4B1, AP3B1 and AP2B1 interact with mitotic checkpoint kinases BUBR1 and BUB1, but the mitotic functions of the association have not been addressed
Comparison of co-expression and PPI search results
Nineteen genes/proteins are ranked high in both Gene Sorter co-expression and POINeT PPI lists (Tables
It is acknowledged that high-throughput data contains intrinsic noises that affect both co-expression and PPI analyses
TRIP13 is a novel kinetochore protein
To further validate the results from bioinformatics studies, subcellular localization was determined for seven candidate genes as preliminary evaluation of their potential mitotic functions. The cDNAs were cloned as GFP-fusions and were all confirmed to express at expected sizes (Additional file
Additional file 8
Figure S1. Western blot of GFP-fusion proteins experimentally tested in this work. Except that C4orf46 was fused with a C-terminal GFP tag, all other constructs contain N-terminal GFP. Asynchronous HEK293 (for TRIP13 and KIAA) and HeLa cells (for the rest) were transfected and cell lysates were harvested 24 ~ 48 hrs later for anti-GFP Western blot.
Click here for file
As mentioned above,
We first confirmed by GST-pulldown that GFP-TRIP13 associates with GST-p31comet in cell lysates (Figure
Figure 1
Interaction between TRIP13 and p31 comet
Interaction between TRIP13 and p31comet. (A) GST-p31comet was expressed either alone or together with GFP-TRIP13 in asynchronous HEK293 cells. The input lysates and GST-pulldowns were probed with anti-GST and GFP antibodies. Untransfected lysates were used as controls. (B) Coomassie blue staining of SDS-PAGE gel loaded with ~0.4 μg of purified recombinant GST-p31comet and His-tagged TRIP13. (C)
Figure 2
Characterization of novel kinetochore protein TRIP13
Characterization of novel kinetochore protein TRIP13. (A) A GFP-TRIP13 transfected HeLa cell in early prometaphase was stained for centromeres (ACA, purple), BUBR1 (red) and DNA (DAPI, blue) to compare with the GFP signals (green). Images shown are maximum projection of a z-series at 1 μm interval. Bar = 10 μm.(B&C) A GFP-TRIP13 transfected prometaphase cell (B) and a cell progressing into metaphase (C) were co-stained with anti-MAD2 antibody and ACA to show GFP-TRIP13 localization only at MAD2 positive kinetochores, such as the one indicated by arrows in (C).(D) A GFP-TRIP13 transfected cell spun onto a coverslip for mitotic chromosome spread preparation was probed with DAPI and anti-HEC1 antibody. (E) A single mitotic chromosome stained with DAPI, anti-GFP and anti-HEC1 antibodies to show kinetochore localization of GFP-TRIP13.
KIAA1377 is a novel centrosomal protein
KIAA1377 was retrieved as a top-ranking non-query protein that associates with centromere/kinetochore proteins in the PPI analyses. KIAA1377 was previously shown to localize at the midbody
Additional file 9
Figure S2. Co-localization of GFP-KIAA1377 with γ-tubulin throughout the cell cycle. HeLa cells transfected with GFP-KIAA1377 were fixed and stained with DAPI (blue) and anti-γ-tubulin antibody (red). In the bottom row, note no bleedthrough of strong γ-tubulin signals to the green channel in the untransfected cell on the left. Bar = 10 μm.
Click here for file
Figure 3
Subcellular localization of GFP-KIAA1377
Subcellular localization of GFP-KIAA1377. HeLa cells transfected with GFP-KIAA1377 (green) were fixed and probed with immunofluorescence. Images at single focal planes are shown. Bar = 10 μm. (A&B) Transfected cells finishing cytokinesis were co-stained with anti-α-tubulin (red in A) or anti-Plk1 antibody (purple in B) and counter-stained with DAPI for DNA (blue). (C) A transfected cell expressing higher level of GFP-KIAA1377 (green) was co-stained for α-tubulin (red) and DNA (blue) to indicate co-localization of GFP-KIAA1377 with microtubule structures. (D) GFP-KIAA1377 is localized at centrosomes. Cells at different stages of cell cycle are distinguished based on anti-centrin 2 (red) and DAPI (blue) staining. The GFP signals in different cells were adjusted to the same scale. Enlarged insets show details of boxed areas
Experimental characterization of other potential mitosis regulators
Five other co-transcription hits were also cloned as GFP-fusions and examined for their subcellular localization. Three were retrieved from both Gene Sorter and CoExSearch searches (Additonal file
PBK/TOPK was previously shown to regulate cytokinesis
Additional file 10
Figure S3. Comparison of subcellular localization of GFP, GFP-CDKN3, GFP-PBK and C4orf46-GFP. Cells undergoing cytokinesis or in interphase or mitosis were probed. DNA is counterstained with DAPI (blue) and microtubules are stained with anti-α-tubulin antibody (red). Note microtubule staining is not always easily discernible because single focal plane images were shown, and the contrast is optimized to show the microtubule bundles at the midbody in cells undergoing cytokinesis. Bar = 10 μm.
Click here for file
GFP-DDX39 and GFP-MELK displayed more interesting subcellular localization. Although the bulk of GFP-DDX39 is diffuse in both the cytoplasm and nuclei, a fraction of the GFP signals co-localizes with γ-tubulin throughout the cell cycle (Figure
Figure 4
GFP-DDX39 is a centrosomal protein
GFP-DDX39 is a centrosomal protein. Asynchronous HeLa cells were transfected with GFP-DDX39 (green), fixed and co-stained with DAPI (blue) and anti-γ-tubulin antibody (red). The cell cycle stages were assigned based on the numbers of γ-tubulin foci and DNA morphology. Bar = 10 μm.
Figure 5
Dynamic subcellular localization of GFP-MELK
Dynamic subcellular localization of GFP-MELK. (A). Distribution of GFP-MELK signals in the cytoplasm and nuclei in G1 and G2 cells. G1 and G2 cells are differentiated based on characteristic staining patterns of CENP-F (red)
In conclusion, we have compiled so far the most comprehensive list of centromere/kinetochore proteins in human cells. Data mining of gene expression and PPI databases using the centromere/kinetochore proteins as queries have retrieved candidate novel mitosis regulators. Experimental validation has discovered two novel centrosomal proteins KIAA1377 and DDX39, and one novel kinetochore protein TRIP13. Functional characterization of these proteins will likely reveal novel mechanisms of mitosis regulation. We conclude that transcriptional co-expression and PPI network analyses with known human centromere/kinetochore proteins as a query group help identify novel mitosis regulators.
Literature search and gene ontology analysis
The list of human centromere/kinetochore proteins were first derived from a previous review
Transcriptional co-expression analysis
The transcriptional expression profiling data in the UCSC Human Gene Sorter (Mar. 2006 datasets) were used for transcriptional co-expression analysis
The co-expression was also analyzed later using the CoExSearch program (
Protein-protein interaction network analysis
PPI network analysis was performed using online tools provided at the POINeT website (
Recombinant DNA, recombinant protein and In vitro protein binding assay
DNA cloning was performed using the Gateway system (Invitrogen)
DNA transfection and immunofluorescence
DNA transfection and immunofluorescence was performed essentially according to
Preparation of mitotic chromosome spreads
Transfected HeLa-M cells were harvested after 16 hr treatment with nocodazole (60 ng/ml final concentration) and swollen for 30 min in 75 mM KCl at room temperature. One millilitre of cell suspension was added to a 35 mm dish containing coverslips, and spun at 1,000 × g for 12 min in a Legend RT-Plus centrifuge (Thermo Scientific) on top of a 15 ml tube holder fitted inside a hanging bucket. The chromosome spreads were then fixed and processed for immunofluorescence.
PPI, Protein-protein interaction; SAC, Spindle assembly checkpoint; APC/C, Anaphase promoting complex/cyclosome; GO, Gene ontology; ACA, Anti-centromere autoimmune.
Competing interests
The authors declared that they have no competing interests.
Authors' contributions
STL conceived the study and performed imaging experiments. AT and STL performed literature search and analyzed retrieved results. AT, PO, SS, ZG and STL performed gene cloning and mammalian expression experiments. KW carried out recombinant protein expression and pulldown experiments. STL wrote the manuscript. All authors read and approved the final manuscript.
We thank Drs. Jianhua Ruan (University of Texas at San Antonio), Justin Ways, Andrea Kalinoski and David Allison (all from University of Toledo, College of Medicine) for discussions. STL was funded by deArce Memorial Fund and NSF grant MCB-1052413.
Re-staging mitosis: a contemporary view of mitotic progression
The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription
FoxM1 is required for execution of the mitotic programme and chromosome stability
Human cyclin A is required for mitosis until mid prophase
The spindle-assembly checkpoint in space and time
The reversibility of mitotic exit in vertebrate cells
Molecular architecture of the kinetochore-microtubule interface
Kinetochore structure and function
The life and miracles of kinetochores
The ABCs of CENPs
Building the centromere: from foundation proteins to 3D organization
The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics
Interactome: gateway into systems biology
Integrative analysis of the cancer transcriptome
Cluster analysis and display of genome-wide expression patterns
Targeting the kinetochore for mitosis-specific inhibitors
Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins
Differential expression of a phosphoepitope at the kinetochores of moving chromosomes
Polo-like kinase 1 creates the tension-sensing 3 F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores
Plx1 is the 3 F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
Cdk1 phosphorylation of BubR1 controls spindle checkpoint arrest and Plk1-mediated formation of the 3 F3/2 epitope
The cohesin complex and its roles in chromosome biology
Condensins: organizing and segregating the genome
The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochores
Gene ontology: tool for the unification of biology. The Gene Ontology Consortium
Sgt1 is required for human kinetochore assembly
The SUMO protease SENP6 is essential for inner kinetochore assembly
A general framework for weighted gene co-expression network analysis
Network theory to understand microarray studies of complex diseases
Coexpression analysis of human genes across many microarray data sets
Large-scale meta-analysis of cancer microarray data identifies common transcriptional profiles of neoplastic transformation and progression
A gene-coexpression network for global discovery of conserved genetic modules
The functional landscape of mouse gene expression
A general co-expression network-based approach to gene expression analysis: comparison and applications
Enumerating the gene sets in breast cancer, a "direct" alternative to hierarchical clustering
HZwint-1, a novel human kinetochore component that interacts with HZW10
A matter of choice: the establishment of sister chromatid cohesion
Mutations in STIL, encoding a pericentriolar and centrosomal protein, cause primary microcephaly
RHAMM is a centrosomal protein that interacts with dynein and maintains spindle pole stability
A small GTPase molecular switch regulates epigenetic centromere maintenance by stabilizing newly incorporated CENP-A
CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1
NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization
CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit
Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP
The human CENP-A centromeric nucleosome-associated complex
Gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphase
Orc6 involved in DNA replication, chromosome segregation, and cytokinesis
Rank of correlation coefficient as a comparable measure for biological significance of gene coexpression
COXPRESdb: a database of coexpressed gene networks in mammals
Replication stress induces sister-chromatid bridging at fragile site loci in mitosis
The hydrolethalus syndrome protein HYLS-1 links core centriole structure to cilia formation
From midbody protein-protein interaction network construction to novel regulators in cytokinesis
Cliques in mitotic spindle network bring kinetochore-associated complexes to form dependence pathway
Shugoshin collaborates with protein phosphatase 2A to protect cohesin
Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase
The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1
Clathrin is required for the function of the mitotic spindle
Clathrin heavy chain mediates TACC3 targeting to mitotic spindles to ensure spindle stability
Interference with BRCA2, which localizes to the centrosome during S and early M phase, leads to abnormal nuclear division
Protein interactions: two methods for assessment of the reliability of high throughput observations
Gaining confidence in high-throughput protein interaction networks
Reliability and reproducibility issues in DNA microarray measurements
Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis
Mouse TRIP13/PCH2 is required for recombination and normal higher-order chromosome structure during meiosis
Mouse HORMAD1 and HORMAD2, two conserved meiotic chromosomal proteins, are depleted from synapsed chromosome axes with the help of TRIP13 AAA-ATPase
Identification of a MAD2-binding protein, CMT2, and its role in mitosis
Towards a proteome-scale map of the human protein-protein interaction network
A human protein-protein interaction network: a resource for annotating the proteome
Two classes of human papillomavirus type 16 E1 mutants suggest pleiotropic conformational constraints affecting E1 multimerization, E2 interaction, and interaction with cellular proteins
A signature of chromosomal instability inferred from gene expression profiles predicts clinical outcome in multiple human cancers
Prognostic breast cancer signature identified from 3D culture model accurately predicts clinical outcome across independent datasets
ATP is required for the release of the anaphase-promoting complex/cyclosome from inhibition by the mitotic checkpoint
p31comet promotes disassembly of the mitotic checkpoint complex in an ATP-dependent process
Dual-specificity phosphatases: critical regulators with diverse cellular targets
A mitotic kinase TOPK enhances Cdk1/cyclin B1-dependent phosphorylation of PRC1 and promotes cytokinesis
KAP: a dual specificity phosphatase that interacts with cyclin-dependent kinases
Dephosphorylation of Cdk2 Thr160 by the cyclin-dependent kinase-interacting phosphatase KAP in the absence of cyclin
The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size
Centrosomal RNA correlates with intron-poor nuclear genes in Spisula oocytes
Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation
A functional analysis of MELK in cell division reveals a transition in the mode of cytokinesis during Xenopus development
CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
IDconverter and IDClight: conversion and annotation of gene and protein IDs
Exploring relationships and mining data with the UCSC Gene Sorter
Large-scale analysis of the human and mouse transcriptomes
A gene atlas of the mouse and human protein-encoding transcriptomes
POINeT: protein interactome with sub-network analysis and hub prioritization
Closed MAD2 (C-MAD2) is selectively incorporated into the mitotic checkpoint complex (MCC)
BUBR1 and Closed MAD2 (C-MAD2) Interact Directly to Assemble a Functional Mitotic Checkpoint Complex
Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis


text:
A keepsake piece of jewelry is a great way to cherish or commemorate a loved one or even a special event. A Locket Necklace is the best way to do just that.
You don't have to put an image in a locket (I put a mustard seed in one of mine), but most people do. So, how do you make sure an image will fit and stay securely in the locket? After you work hard to find the perfect image, you want it to stay put. It isn't tough, it just takes a little bit of prep work.
How to size an image for a locket:
This is an easy one because you are sure to have these items in your house already. Lay a piece of clear plastic (ie. sandwich baggie) in the opened locket. With a marker, draw around the inner edge of the locket to create a template for the image size. Cut out the template and place it over your already printed image. Using the template as a frame, cut the image to fit.
Now that you have your image cut out to the appropriate size and shape, you want to make sure it doesn't fall out of the locket.
Here are 3 easy ways to make sure your image remains secure:
1. Drop a tiny bit of glue in the locket and place the image on the glue. You have a little bit of time to slide the image around to make it straight. That seems too easy doesn't it?
2. Cut a small piece of double sided tape and put it on the back of your image. Carefully lay the tape-side down in the locket. This can be a little harder. If you don't lay down the image in the exact spot you need it, you should be able to gently lift it off the locket and try again.
3. Print your image on sticker paper (such as label paper). Cut the image, peel off the protective backing and place the adhesive side directly into the locket. This will not be as forgiving as the glue or double sided tape. I suggest that you lay the edge of the image down first and then roll it down from there.
With a steady hand a couple of minutes of prep work, you can have a beautiful keepsake just like this locket with my favorite little Frenchie.
Other than a picture, what have you put in your locket?
To see more of Olive Yew's personalized necklaces, bracelets, rings and earrings, visit our Etsy shop.


text:
Futuris Corporation Limited
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A technical analyst might call Futuris's share price 'rangebound'. It dipped down in issue 188/Nov 05 (Long Term Buy—$1.91), where we upgraded our recommendation. Now, with the stock up 14%, we're downgrading...
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Bangalore Metro Rail Corporation Limited Vacancy 2016
BMRC has issued the Maintainer, Operator & Engineer Posts Recruitment on 11 June, 2016. So Now Job Seekers can fill the application form BMRC Recruitment 2016 Exam. BMRC will conduct the Maintainer, Operator & Engineer written exam on 18 Sep, 2016. Aspirants who had applied application from for Maintainer, Operator & Engineer Exam, They can fill their BMRC Online Application Form before the last date of application form.
Rajasthan 10th Board Results 2016, RBSE Exam Roll Number wise result
RSGSM Recruitment 2016, 81 Chief Engineer, Officer & Other Posts Apply Online
About BMRC in Details
Bangalore Metro, is a metro system dollop the city of Bangalore in Karnataka, India. NAMMA (Hamari) Metro is India's second biggest metro system in terms of both length & number of stations, after the Delhi Metro. It also incorporate the first underground metro line in South India. The Metro network involve two color-coded lines, with a total length of 31.52 kilometers serving 30 stations. The system has a mix of underground, at size, and exalted stations using standard gauge tracks. The metro has an average daily ridership of 140,000 Passengers.
Name of The Corporation
Bangalore Metro Rail Corporation (BMRC)
Name of The Posts
|Maintainer, Operator & Engineer
|No. of The Posts
308 Posts
Opening Date of Form Filling
|11 June, 2016
|Closing Date of Application Form
11 July 2016
Written Exam Date
18 Sep, 2016
Eligible Criteria of BMRC Recruitment 2016
Age Limit- Applicant's age limit should be minimum 35 years on 11 July 2016. Upper age limit should be applicable for the reserved category candidates as per karnataka governments rules and regulations.
Educational Qualification- Aspirants must have 10th Class with Diploma in Engineering in related Subject from any recognized Institution / University with minimum qualifying percentage of marks. Post wise academic information will be duly given in official notification.
Exam Fee- Aspirants need to pay of Rs 700/- for General & OBC Category & Rs 300 for SC, ST & Ex-Serviceman category candidates.
Selection Process- Applicant's selection is based on the performance on written test, Kannada Test & Medical Test.
Vacancy Details-
Post's Name
No. of Posts
|Train Operator
Section Engineer
DMRC Maintainer, Operator & Engineer Post Jobs 2016
The BMRC has released the BMRC Recruitment Application Form 2016 on its official portal on 11 June, 2016. The BMRC has Conduct the Maintainer, Train Operator,& Engineer Exam 2016. Aspirants are busy in fill their application form. So they can fill application form through given instructions.
How To Fill Application Form of BMRC Jobs 2016
- Firstly, Applicants visit on the official Portal @ www.bmrc.co.in.
- Then click on the 'Recruitment' link.
- Now Search the BMRC Recruitment 2016 Application Form link.
- Fill the all required fields in the form & submit the application form.
- Applicants can download & take a print out of this form for future use.
Important Note- If any aspirants want full information related to this recruitment then go to the official website where you found your answer.
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Blog Posts Tagged with "3G"
Homemade Spy Drone Cracks WiFi and GSM Networks
August 01, 2011 Added by:Headlines
"WASP is equipped with the tools to crack Wi-Fi network passwords made possible by an on-board VIA EPIA Pico-ITX PC running BackTrack Linux equipped with 32GB of storage to record information. WASP can also act as a GSM network antenna meaning it will be able to eavesdrop on calls/text messages..."
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Skype Fixes Critical Android Application Vulnerability
April 22, 2011 Added by:Headlines
"After a period of developing and testing we have released a new version of the Skype for Android application onto the Android Market, containing a fix to the vulnerability reported to us. Please update to this version as soon as possible in order to help protect your information..."
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Mobile Devices Continue to Attract Cyber-Scamsters
April 17, 2011 Added by:Rahul Neel Mani
While it's only beginning to percolate, a trend is clearly emerging— cyber criminals are looking for new opportunities outside of the PC environment. They are investing more resources toward developing exploits that specifically target users of mobile devices...
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Where's My Creeper Box?
February 13, 2011 Added by:Dave Porcello
Indeed, after 7 years my long-coveted creeper box has finally arrived. And nothing says Sneakers like a text message from an elegantly-placed drop box as you exit your target facility's parking lot in an unmarked utility van...
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Intel Processor Chips Now 3G "Kill Switch" Enabled
December 21, 2010 Added by:Headlines
SMS kill-switch enabled devices may be vulnerable to unauthorized data deletion or susceptible to being disabled if the systems regulating the mechanism are breached by hackers or by way of an insider threat...
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Hacker to Release Symantec's PCAnywhere Sour... Jerry Shaw on 10-05-2015
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PoS Malware Kits Rose in Underground in 2014... on 03-17-2015
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New PCI Compliance Study... on 03-17-2015
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- How Does UC in the Cloud Impact Your Security Posture?
- How to Prevent Ransomware and Cyberattacks
- SAP Cyber Threat Intelligence report – July 2017
- WannaCry: How We Created an Ideal Environment for Malware to Thrive, and How to Fix It
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- Convenience Comes at a Steep Price: Password Management Systems & SSO
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i. The NATO Parliamentary Assembly supported calls for NATO to prepare a new Strategic Concept well before the April 2009 NATO Summit in Strasbourg/Kehl when NATO leaders formally decided to produce one. The previous Strategic Concept was adopted in 1999 and the security environment has altered profoundly since that time. NATO – as a political and military alliance – has remained a vital institution precisely because it has adapted to changing circumstances. That process of continual adaptation cannot take place in the context of a static Strategic Concept. As the strategic environment and NATO evolve and adapt, the Strategic Concept must be revised periodically and renewed.
ii. The new Strategic Concept will be the first one to be adopted after the terror acts of 11 September 2001 which transformed our understanding of the new security challenges posed by international terrorism. It will also be the first Strategic Concept to incorporate the perspectives of the new Allies – many having been formerly under Soviet rule - which have acceded to the Alliance over the last decade. It must therefore, combine more perspectives on how to address both traditional and new threats to mutual security.
iii. The Allies also represent different points of view on a broad range of issues that affect the future of the Transatlantic relationship. There are, for instance, competing "visions" of the relationship between NATO and the European Union. There are also concerns among NATO members about inequitable divisions of labour and levels of commitment to NATO operations. Such problems further complicate the task of maintaining public support for NATO and its operations.
iv. The NATO Parliamentary Assembly is well placed to make recommendations about a new – or at least, revised - Strategic Concept. Its members are drawn from the national legislatures of all of the member states of the Alliance. It is a product – and a symbol - of the democratic nature of the Alliance and represents a direct link to the citizens of the member states. As such it is a two-way channel of communication that affords NATO officials the opportunity to speak directly to the representatives of the citizens of the Alliance as well as a venue for parliamentarians to communicate their constituents' concerns to NATO's leadership. As such, the contribution of the Assembly represents many of the concerns of the people this Alliance was created to defend.
v. This document is the product of extensive consultation within the Assembly involving all five of its Committees. Although Assembly members represent very diverse political perspectives, consensus emerged on certain key themes in debates on the new Strategic Concept, including:
• the enduring importance of collective defence Article 5;
vi. This document begins by presenting the main recommendations which emerged from the Assembly's deliberations. This is followed by Appendix 1 which provides some background on the issues and discussions that underpin the recommendations.
Finally, the text of the North Atlantic Treaty itself is reproduced as Appendix 2.
To read more, download the full document


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My name is Renate Henschke and I am an Australian fashion & costume designer/maker working in both Ireland and Australia
I am a designer of costumes, props and sets for film, television and theatre.
Please contact me if you have any 3 dimensional design challenges.
Ireland +353 (0)86 307 2294
Australia +61 (0) 422814963
Skype renatehenschke
E renatehenschke[at]gmail[dot]com


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NPR: Young Men Stuck in Adolescent-Adult Limbo It wasn't long ago, Hymowitz argues, that the average man in his mid-20s had achieved many of life's major milestones — he had a job, a marriage, perhaps even kids and a house. Today's mid-20something male "lingers happily," Hymowitz writes, "in a new hybrid state of semi-hormonal adolescence and responsible self-reliance." Social scientists are struggling to define this new phase of life — "emerging adulthood" and "delayed adolescence" are two ide … [Read more...]
NPR on the "child-man" phenomenon
January 28, 2008 by Leave a Comment
Abbu finally gets to feed baby
May 7, 2006 by Leave a Comment
[This is a repost. It occurred to me that my previous title, "Abbu gets to finally get some of the nursing action", could be misconstrued. Sorry about that...] Well, a bleary eyed Ammi finally allowed Abbu to break out the bottle and feed Raihana Friday night. It was masha'Allah a wonderful experience. Not only was it an incredible relief to actually be able to do something when little Raihana's wails of hunger reached a thunderous roar, but the sight of her looking up at … [Read more...]