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Know more about Angelina Jolie`s mastectomy decision
Oscar-winning film star Angelina Jolie revealed that she underwent a preventive double mastectomy after discovering that she has a mutated version of the BRCA1 gene that made it extremely likely she would get breast cancer.
Jolie and partner to Brad Pitt made the announcement in the form of an op-ed she authored for Tuesday's New York Times under the headline, "My Medical Choice." She wrote that she finished three months of surgical procedures to remove both breasts as a preventive measure.
Breast Cancer Awareness Campaigners believe that Angelina Jolie's openness would help raise crucial awareness of genetic breast cancer risk.
What is Breast Cancer?
Breast cancer is a malignant growth that begins in the tissues of the breast. Breast cancer mostly common in women, but it can also originate in men.
There is however no exact cause of breast cancer, but myths in this case is more widespread than the real cause.
Causes of Breast Cancer
Age: Age plays an important role in determining the chance of getting breast cancer – chances of contracting the disease increases with age.
Genetic Inheritance: Family history like that of mother, sister or daughter been diagnosed with breast cancer further increases the risk.
Early menses or menopause: Early start onset of menses and/or early menopause can also be a reason associated with breast cancer.
What is Double Mastectomy?
Mastectomy is the medical term for the surgical removal of one or both breasts, partially or completely. Mastectomy is usually done to treat breast cancer.
Types of Mastectomy
The type of mastectomy a person undergoes depends on each individual case and on factors such as size, location and behaviour of the tumour.
Nipple-sparing mastectomy: It involves surgical removal of breast tissue.
Skin-sparing mastectomy: This process involves removal of the entire breast except for the breast skin.
A simple mastectomy: It involves removal of the entire breast tissue, but the axillary contents are undisturbed.
Modified radical mastectomy: In this process, the entire breast tissue is removed along with the axillary contents.
Radical mastectomy: It is also known as halsted mastectomy. This procedure involves removing the entire breast, the axillary lymph nodes and the pectoralis major and minor muscles behind the breast.
Extended Radical Mastectomy: Radical mastectomy with intrapleural en bloc resection of internal mammary lymph node by sternal splitting.
A double mastectomy reduces a mutation-carrier's risk by about 90 percent to a level lower than that of women who don't carry the genetic flaw. But it but can never eliminate the danger entirely.
Mostly women with breast cancer go in for plastic surgery. A breast implant is a medical prosthesis used to augment, reconstruct, or create the physical form of breasts. Previously doctors used to shape new breasts by taking fat from a patient`s stomach, upper back, buttock or thigh, but now the procedure has been made safer and more comfortable.
Surgery is often combined with other treatments such as radiation therapy, chemotherapy, hormone therapy and/or monoclonal antibody therapy.
Compilation by: Shruti Saxena


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QUEBEC-RESEARCH-L ArchivesArchiver > QUEBEC-RESEARCH > 2005-06 > 1118059525
Subject: Re: [Q-R] Meaning of Half Value
Date: Mon, 6 Jun 2005 08:05:25 EDT
In a message dated 6/6/2005 2:12:28 A.M. Eastern Daylight Time,
Thanks Owen, When I use the online translator it is generally to get an idea
of what the page says. I really wish I could find that page again. If not I
guess it gets to remain a mystery. I wish there was a better way of
translating French to English when one is not privileged with that skill.
You're welcome Debra.
There is a better way of obtaining a translation from French to English, or
English to French: Post the text to the List, and one of us who are
"privileged" to be bilingual will be happy to translate it.
Fr. Owen Taggart
This thread:

subset: commoncrawl


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Potsie's Creamed Chicken and Biscuits Casserole
Recipe by Pot Scrubber
This is a wonderful and simple to prepare comfort food using things you probably already have around. Kids love it- but so do adults. At least, in this home. Try it. It is one of my quick and easy favorites. This is my first submitted recipe... be kind.
Top Review by NanaRLL
Potsie, this dish was FABULOUS! I doubled the recipe and added in 1 cup of evaporated milk along with the amount of regular milk called for. I used half mayo and half sour cream, and chose to use cream of celery soup. I added in a bit of flour as suggested for a thicker sauce, and also some partially micro waved frozen peas and carrots. I thought I had pimentos on hand but couldn't locate them when I needed them most - someone had to have nabbed them - but I can't imagine for what purpose because I'm the only one around here who actually cooks - so I had to woefully omit them. I added extra onions to compensate for the missing pimentos, then I added a few generous shakes of garlic powder, salt and pepper, and the chicken. I added a bit more of the chicken than specified because it seemed really saucy. Then, since it seemed so saucy, I sprinkled the shredded cheese on top before placing it in the oven for the first 20 minutes - so I would have a firmer foundation for the biscuits I would be placing on top later, because I was afraid the bottoms would be soggy sitting in all that sauce. After it was bubbling nicely I sliced my large-size biscuits in half through the middle so they would be thinner and placed them on top of the cheese, then sprinkled them with jar-type Parmesan cheese and popped the dish back in the oven. Then I stood there and drooled while my grandchildren - and my adult children - all tried to crowd around the stove to watch the dish through the oven-door glass while it bubbled away, so they could try to gage how much longer it would take to be done. Like they would know. Anyway, everyone absolutely loved it and were so busy scarfing it down that it was actually quiet around the dinner table for a change. But I must give a word of caution to others - don't make the mistake I did by adding in evaporated milk - this dish was VERY rich and would have been so even without it. And even though it looked overly saucy when I first placed it in the casserole dish, a lot of the sauce was sucked up by the chicken and veggies, so we had less sauce than we wanted after it was done baking. Again, my mistake for adding in the extra chicken - next time I'll control myself no matter how saucy it seems before baking it, and add the amount of chicken specified. I should have followed your directions to the tee, and I will in the future, Maestro Potsie. By the way, the biscuits turned out perfect and weren't a bit soggy, and I served this dish with a side of boiled and buttered red-skinned potatoes. I can't wait to make this again - with the pimentos - which I'll hide when I bring them home - and hope I'll be able to remember where I hid them when I need them again. Yeah, right! Thanks so much for this great comfort dish!
See Discussion:142 Reviews 18 Tweaks
Ingredients Nutrition
- 1 small onion
- 1 1⁄2 teaspoons butter
- 2 lbs chopped cooked chicken breasts or 2 lbs boneless skinless chicken thighs
- 1 (10 3/4 ounce) can cream of chicken soup
- 1⁄2 cup mayonnaise
- 1 cup milk
- 1⁄2 cup chopped pimiento or 1⁄2 cup sauteed red bell peppers or 1⁄2 cup jarred roasted red pepper
- 1 1⁄2 cups shredded mild cheddar cheese
- refrigerated biscuit
Directions See How It's Made
- Preheat oven to 350.
- Grease the bottom and sides of an 11x7 inch baking dish or 10 inch quiche dish.
- Chop the onion.
- Heat butter in a small nonstick skillet and saute until tender.
- Combine cooked onion, soup, chicken, milk, pimiento, and mayo in a bowl and then turn into the prepared baking dish.
- Bake for 15 minutes and remove from oven.
- Sprinkle 1 cup of the cheese over the baked mixture.
- Arrange biscuits decoratively in a single layer and sprinkle remaining 1/2 cup cheese over them.
- Return casserole to oven and bake until biscuits are golden brown and sauce is bubbly, usually about 20 minutes longer.
- NOTE: 02/16/09: This is RICH! You may sub sour cream for all or part of the mayo if you wish to cut back on the heaviness. The last time I made this I used a 1/4 cup of fat free mayo and 1/4 cup of sour cream. I think it was delicious! Also -- I took the suggestion of adding a cup of cooked green peas. What a terrific idea! I LOVED it! I think I might add two cups next time. Thanks to you all for making my humble recipe and leaving your suggestions. You GREATLY improved a recipe I was already proud of. Thanks so much.


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The Latest News On The API Economy
Searching: No Search Term , Filtered By API: "Tinysong"
34 Streaming APIs: Tinysong, 7digital and Playme
Our API directory now includes 34 streaming APIs. The newest is the Furk.net API. The most popular, in terms of mashups, is the Tinysong API. We list 6 Tinysong mashups. Below you'll find some more stats from the directory, including the entire list of streaming APIs.


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The Eagles and Grizzlies stayed even throughout the first three periods, but Utah finally nabbed a 3-2 overtime win over Colorado in Loveland, Colo., thanks to Nick Tuzzolino's power-play goal.
Tuzzolino also had an assist in the win, and goalkeeper Adam Morrison stopped 37 shots.
Adam Estoclet assisted on Tuzzolino's game-winner and another Grizz score.
Gaelan Patterson and Ian Schultz scored Utah's other goals.


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Together National Priorities Project, Peace Action and a terrific group of colleagues, have assembled all the materials you need to enter the current national debate about Pentagon spending, while building the capacity of colleagues and allies to join in as well.
We are excited that groups and local organizers will use this curriculum to train grassroots activists. We believe that our life experiences, combined with solid information, are the key ingredients to building a politically empowered movement to change federal spending priorities.


text: You really should check out darcs. I did an extensive arch/darcs comparison, and the XWT project (xwt.org) is going with the latter. Vastly simpler than either bk or arch but with all the same features. - a ebiederman at lnxi.com (Eric W. Biederman) writes: > This is just a heads up. I have been doing some investigation > into distributed version control systems. arch looks like it > usable and getting more usable. > > >From the design standpoint I don't see any big issues and > it appears to be the equal of bk etc. I think I see some > was to make it simpler and more powerful but that is not needed to > make a good revision control system. > > I need to do a little more testing to see what arch is actually like > to use. Before I seriously start advocating anything but I'm not > expecting any weird issues to crop up. Except possibly for some > issues running under cygwin, for the platform challenged. > > Once my evaluation is finished I plan on pushing the projects I work > on in that direction. So this is something to think about. > > Eric > _______________________________________________ > Linuxbios mailing list > Linuxbios at clustermatic.org > http://www.clustermatic.org/mailman/listinfo/linuxbios > -- "If Darl McBride [the CEO of SCO, who claims the GPL 'destroys intellectual property'] was in charge, he'd probably make marriage unconstitutional too, since clearly it de-emphasizes the commercial nature of normal human interaction, and probably is a major impediment to the commercial growth of prostitution" -- Linus Torvalds


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What makes our package special is that we can offer you the opportunity to use this fabulous resort as your base from which to explore not only the Kruger Park, but also the scenic province of Mpumalanga and even Swaziland
One full day game drive on an open safari vehicle in the Kruger Park is included in the rate. A guided day tour of the Panorama Route is also included in the tour price, departing from the hotel. The rest of the time is yours to spend at your leisure.
The scenic Panorama Route is famous for its spectacular views, waterfalls and beautiful rock formations. You can also visit an African craft market and cultural village nearby.
Various optional extras can be arranged, including guided bush walks in the Kruger Park, and night drives with powerful search lights on an open safari vehicle, looking for leopards, lions and other nocturnal animals. A variety of adventure activities are available in the area, as well as hot air balloon flights, horse trails and even elephant back rides.
There is no fixed itinerary for this semi-guided safari. The itinerary below is an example and can be adjusted to suit your requirements.
Tour Itinerary:
Day 1: The safari departs from the Nelspruit airport in Mpumalanga, which is close to the Kruger National Park. Daily flights can be booked from Johannesburg to Nelspruit, on a number of different airlines. You will be met at the airport upon arrival and transferred to the hotel resort, on the border of the Kruger National Park. Take the afternoon to settle in and explore your surroundings. Dinner will be announced by drumbeat.
Day 2: We rise before the sun to enjoy a full day game drive in the Kruger National Park, or perhaps a morning and afternoon game drive if you prefer. The day is filled with the excitement of seeing Africa's wildlife in their natural habitat, from the comfort of an air-conditioned vehicle. Whether it's lion, elephant, giraffe or the variety of birds and smaller animals that can be seen, we guarantee you a fascinating and satisfying safari experience in this vast African wilderness area. While looking for the big 5 and other wildlife, we stop at waterholes, view points and picnic sites where you can stretch your legs and enjoy some refreshments.
Day 3: Today is devoted to a tour of the spectacular Panorama Route, the most scenic part of the Lowveld. We visit God's Window and other breathtaking view points, Bourke's Luck potholes, the Blyde River Canyon, various magnificent waterfalls and more. We return to the hotel resort around sunset.
Day 4-5: Spend the day at leisure to explore and enjoy the many facilities on offer at the resort, or perhaps book an extra game drive, day tour or one of the optional activities available in the area.
Day 6: After a relaxed breakfast at the lodge, it is time to pack up and be transferred back to Nelspruit airport, in time for your flight to your next destination.
Price upon request.
About This Supplier
Also see tour packages in:
Africa South Africa Nature & Wildlife National Parks
Email it to a friend:
Click here to email this vacation to a friend


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Wed Oct 23, 2013, 12:08 PM
Savannahmann (3,891 posts)
The truth is that Death Panels have always existed
Rationing is the means of adjusting supply and demand. Deciding who gets what is another way. We've all seen the medical shows where they discuss a patient who is on the heart transplant list. So we'll use that as an example. The list is hundreds, or thousands of patients long. A panel of doctors discuss your particular situation, and place you on the list according to many factors. Age, other health issues, what caused your heart problems, and other things. A man who has a genetic defect and works full time with a family and two small children who has been working hard to protect his heart knowing of his genetic issues is going to place higher than a man of the same age who is a drug abuser and has nearly exploded his heart with Crack.
That of course makes sense. There are a finite number of hearts, a finite number of Doctors who can perform the surgery, and a rather large list of people who need the lifesaving procedure. We have to decide the issue somehow, and this is the best way we can come up with. That drug user mentioned above probably wouldn't even make it on the list in all honesty.
Now, that is the situation for those with insurance, or money. What the ACA does is provide more people with the opportunity to make it on to the list, where they might get the surgery they desperately need to survive. The total number of people who get the surgery probably won't go up that much, but the ones who had been excluded before will now have a chance. If you are low on the list, chances are you won't get the transplant. If you don't get on the list, there is no chance for a transplant. That is the death panel, if you don't make the list, you are dead, often quite soon.
The truth is that death panels have always existed. The truth is that we've used them ever since the first doctor came up with a treatment that seemed to work. Look at Senator Kennedy. He got surgery on his brain from the one hospital that had any success with it. Others were rejected as candidates, because of any number of factors. The most limiting one was the Doctor's time, he can only treat so many patients, and he can only train so many at one time in the procedure.
Orbis is a charity that operates a flying eye hospital. http://www.orbis.org/ They fly this hospital into remote areas, and perform a number of surgeries on peoples eyes restoring sight. Cornea transplants and other treatments. They have thousands of people who would love to have the surgery, and they do as many as they can, but they can't treat everyone. They use the judgment of the Doctors, who would benefit the most, who has the best chances. All of it is judgment of one or two Doctors.
The people of Orbis would love nothing more than to restore sight in every single person in the world who needs their services, but they are human, and they are limited. They do a great job, and they are worthy of commendation. But for every person who can see because of the surgeries this group performs, dozens go without that procedure.
Supply and Demand, someone has to decide who gets what, and that has been the case since the dawn of time. The objection to the ACA is that the decisions will be harder now, because more people will be eligible to get on the list in the first place.
1 replies, 700 views
Always highlight: 10 newest replies | Replies posted after I mark a forum
Replies to this discussion thread
|1 replies
The truth is that Death Panels have always existed (Original post)
|Oct 2013
|Oct 2013
Response to Savannahmann (Original post)
Wed Oct 23, 2013, 06:19 PM
Savannahmann (3,891 posts)
1. Generally I never do this.
This is a first for me, kicking my thread, once, and only once, to get the evening crew a chance to read and comment. If it drops again, I'll let it be. But what I was wondering is this, why haven't we told this to the people to counter the claim of Death Panels from the RW? That they have always existed is a truth we should be mentioning, and pointing out how the ACA is just giving more people a chance to live instead of automatic death.


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McCartney, 24, has had the problem for 10 months and decided to go under the knife after seeing a specialist.
Boss McCarthy told the club website: "We have taken every conservative measure to solve the problem and the last course of action was surgery.
"Hopefully it will put an end to what has been a frustrating injury."
The full-back visited specialist Dr Nic Maffuli at the North Staffordshire Infirmary to ascertain the best course of action.
He will have the operation on Monday and is unlikely to be back within four months.


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Negativity in the work place can be like a bad virus. When working with a small team, it's important that you nip negativity in the bud as early as possible.
If you see a potential outbreak and fail to jump in and take care of matters you can often find yourself knee deep in, well, something you don't want to be knee deep in. Once negativity gets out, it becomes that much more difficult to get back under control. It soon spreads from one person to the next to the next and soon you realize that you've got an office full of Negative Nancyies with bad moral. (No offense to anyone named Nancy!)
What a lot of people fail to realize is that attitude is a choice. We have a choice in any given situation, toward any particular person or about life in general to choose to be either positive or negative about it.
Now we may not wake up in the morning and say to ourselves, "I'm going to be a grumpy SOB today." But when someone brings a bad attitude to the office, it was their choice to do so. It's up to each person to decide how they'll react as life takes it's course.
It ain't always easy being nice
We are all negative from time to time and we all have our downer moments, but in the office, especially where a few people are sharing a single work area, constant negativity can have a drastic effect on productivity. If not dealt with, it doesn't take long before a negative perception soon begins to take hold and spread from the source to others in the office until, rightly or wrongly, the office is infected with a particularly harmful viewpoint.
While thinking positively is a choice, for some it can often be the more difficult choice. This is usually due to a string of other choices made previously. If you fill your mind with enough negativity it becomes difficult to think positively. If that's you (and it can certainly be me at times) you've got to break the cycle of negativity. But in order to think positively one much choose to do so. You have to make a deliberate choice think positive about things that you have a built in tendency not to. You must also choose fill your mind with positive things. You have to choose to shut out negative words and influences and choose to stop and think before reacting in order to react properly.
This is a daily process. It's not something you choose once, but you have to choose every day. While nobody wakes up and chooses to be a grump, we can wake up and choose to be positive. After reading the book How to Win Friends and Influence People I made a concerted effort to be a more positive person in the office. I'm not a negative person per se but I am one that likes things to go my way (read: control freak). Initially I did great at lengthening my fuse, reacting less abrasively and letting things that annoyed me just roll off my shoulders. But then I stopped trying. I stopped making the effort assuming that it would all come naturally. It didn't.
My fuse once again became shorter, my annoyances more obvious and the way I would talk to my employees slightly less friendly. But here again, I realized that I could be handling things better. Not making a concentrated decision to act in a positive manner was affecting my interaction with my team. And it was affecting my team member's interaction with each other. I realized again, things had to change.
Changing negativity requires changing our perspective
While we all have our justifications for being negative (I generally find it a source of humor) most often we just need to look at things from a different perspective in order to change the negative into a positive. In fact, things that we often assume are negative about other people, we spin into positive traits for ourself. Here is a fun little bit found in John Maxwell & Jim Dornan's book, Becoming a Person of Influence.
When the other fellow takes a long time, he's slow.
When I take a long time, I'm thorough.
When the other fellow doesn't do it, he's lazy.
When I don't do it, I'm busy.
When the other fellow does something without being told, he's overstepping his bounds.
When I do it, that's initiative.
When the other fellow overlooks a rule of etiquette, he's rude.
When I skip a few rules, I'm original.
When the other fellow pleases the boss, he's a brown-noser.
When I please the boss, it's cooperation.
When the other fellow gets ahead, he's getting the breaks.
When I manage to get ahead, that's just the reward for hard work.
If we can all start thinking about others the way we do ourselves, we'll be a lot better off. Instead of thinking of your fellow co-workers as losers, suckups and flakes, look at things from your own perspective. It might give you a whole new perspective.
Overall, though, we just need to start being more positive. Just remember, though that while negativity comes easy, being positive is the more difficult choice. and you've got to choose to be positive today, and again tomorrow, and the next day, and the next day and so on.


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The Nicholas Kirkwood x Keith Haring Collaboration is Out!
Nicholas Kirkwood is a bonafide shoe genius. And Keith Haring revolutionized the art world in the '80s and '90s by helping to show that activism and art could meld into bright, beautiful creations. So when it was revealed that Kirkwood would be creating a collection of shoes in honor of Haring, well, we couldn't wait to see them. Sadly, his knee-high rollerskate boots aren't for sale — they're only on display at the Arnhem Mode Biennale, but we could imagine trying to DIY our own pair of high-heeled wedges with some day-glo paint some time soon. [W Magazine]


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Weekend Brunch: Poached Eggs on Roasted Potatoes with Hollandaise SauceMarch 5, 2010 | By | COMMENTS
Last weekend, I decided to make a very ambitious breakfast of poached eggs on roasted potatoes with Hollandaise sauce. It took a whole carton of eggs (three for the Hollandaise, four for poaching and the rest for throwing away after the yolks bled into the whites) but the resulting dish, as you can see, was pretty dazzling. It's the kind of breakfast that makes you, the chef, feel proud and triumphant, roaring with the might of a culinary lion. "I made that!" you keep saying to yourself, reluctant to disturb the plate with a fork. "I really made that."
"Yes," says your companion, digging in.
"I'm a culinary lion!" you continue. "Rawwwwwwwwr!!"
You may get strange looks after that, but just ignore them: you deserve your moment in the sun.
This dish involves three separate techniques that, when they come together, form a beautiful whole. Let's take them one at a time.
Technique One: Roasting The Potatoes
To roast the potatoes for this dish, I used a recipe I'd been eager to try since I read about it on Food52: Tad's Roasted Potatoes.
Tad Friend, brilliant New Yorker writer and Amanda Hesser's husband, developed a roasted potato technique that relies on one essential ingredient: old potatoes. Explains Hesser: "As potatoes age, their starch turns to sugar, making them denser, softer and easier to caramelize."
In January, I bought a bag of white potatoes for this very purpose and finally, this past weekend, I put them to use:
You preheat your oven to 375 and cut your potatoes into little cubes (3/4 inch cubes, according to the recipe, which is why you see that ruler there.) You only want to cut up enough to form an even, flat layer in your cast iron skillet.
Drop the potatoes in there and then coat generously with olive oil (a heavy pour). Season with salt and pepper and drop in 3 garlic cloves and 8 sprigs of thyme or rosemary. Toss around and flatten once again.
Place in the oven and roast for 40 minutes, scraping and turning the potatoes every ten minutes. The potatoes, when done, should be a beautiful dark brown and cooked all the way through (taste one):
That's it for the potatoes! Now on to the next technique.
Technique Two: Hollandaise (in a Blender)
The hardcore cooks among you might scoff at this shortcut for making Hollandaise sauce, but would you scoff if I told you that I learned this technique from a certain cookbook called "Mastering The Art of French Cooking" by Julia Child? I didn't think so! Yes, right there on page 81, Ms. Child has a recipe for "Hollandaise Sauce Made in the Electric Blender." It's wonderfully easy.
In the blender, place 3 egg yolks, 1/4 tsp salt, a pinch of pepper and 1 to 2 Tbs lemon juice.
Then cut up one stick of butter and melt it in a little pot until it's foaming.
Turn the blender on and carefully drip the hot, melted butter into the whirring egg yolks (you may need to use a towel so it doesn't splash up and burn you). Go slowly: drop by drop as it emulsifies and then a thin stream after that. Julia says: "By the time two thirds of the butter has gone in, the sauce will be a thick cream. Omit the milky residue at the bottom of the butter pan. Taste the sauce, and blend in more seasonings if necessary." (I added more salt, pepper and lemon juice after tasting it.)
That's it! Easy, breezy Hollandaise:
Technique Three: Poaching Eggs
No pictures here: this is an unpleasant, ugly process. And I can't claim any expertise here–it's the kind of technique you just have to do and do and do again (as illustrated in the "Julie & Julia" movie.)
Here's what I did: I filled a Dutch oven with water and carefully raised it to just under a simmer (a little bubble every now and then). I filled a separate bowl with ice water. I added salt to the hot water (just a bit) and a splash of vinegar (red wine, though white wine vinegar is preferable). I cracked each egg into a tiny bowl and carefully slipped the egg into the almost-simmering water by getting the bowl as close to the surface of the water as possible. Then, immediately, I used a spoon to coax the white around the yolk. There were lots of strands and ugly things floating around, but I ignored them.
Four minutes later, I lifted the egg with a spider into the ice water. This stops the cooking and also rinses the vinegar off the egg. I did this with the rest of the eggs (and they all, somehow, managed to stay together). Then I emptied the pot of the water and the white floaty stuff, cleaned it, refilled it with water, brought it up to a simmer and reheated the eggs.
This was certainly the most tedious part of making this breakfast but also the part I'm the most glad about. The fact that the poached eggs were solid on the outside and runny on the inside was a real culinary coup and it made the breakfast as enjoyable as it was. Here it is, again, in all its glory:
Do you dare attempt it this weekend? All you need are potatoes, garlic, thyme, a carton of eggs and a dream. The rest will take care of itself.
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This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Calyculin A (Caly A) is cell permeable toxin widely used in cell biology research as an inhibitor of type 1 and type 2A protein Ser/Thr phosphatases of the PPP family. Here we tested effects of low concentrations of Caly A on proliferation of human cancer and non-cancer cell lines. We found that long-term 0.3 nM Caly A prevented G1 to S phase cell cycle progression in human Hs-68 fibroblasts and ARPE19 epithelial cells, but not human breast cancer MDA-MB-468, MDA-MB-231 and MCF7 cells. These conditions produced no change in cyclin D1 levels or in the phosphorylation of endogenous proteins. However, acute application of 0.3 nM Caly A blocked serum-induced increase in intracellular calcium levels in Hs-68 fibroblasts, but not in MDA-MB-468 breast cancer cells. We propose that subnanomolar Caly A prevents cell cycle progression because it blocks calcium uptake by fibroblasts. This probably involves non-selective cation channels and cancer cell proliferation was not affected because calcium enters these cells by other channels. Our results suggest that calyculin A has dual actions and acts as a channel blocker, in addition to its well-established effects as a phosphatase inhibitor.
Calyculin A (Caly A) was initially isolated from a marine sponge Discodermia Calyx, collected in the Gulf of Sagami in Japan. Caly A is potent inhibitor of starfish development and acts as a cytotoxic compound against L1210 leukemia cells [
Caly A is a complex natural product with 15 chiral centers. Study of the structure-activity relationship identified several functional groups, such as the phosphate group, the hydroxyl at C-13, and the hydrophobic polyketide tail, as essential for the inhibitory action on the PPP phosphatases [
We have previously demonstrated that low nanomolar doses (10–50 nM) of Caly A induced T286 phosphorylation and proteasome-mediated degradation of cyclin D1 in MDA-MB-468 and MDA-MB-231 human breast cancer cells [
To further investigate the specificity of Caly A in this study we used lower doses and compared the response of breast cancer with human Hs-68 fibroblast and ARPE-19 retinal epithelial cell lines. These cell lines were selected as commercially available (ATCC, CRL-1635 and CRL-2302) human cells that have normal karyotype and serum-dependent growth, to serve as immortalized but non-transformed controls. To our surprise we observed that at extremely low doses of Caly A (<1 nM) non-cancer fibroblast and epithelial cells were arrested and did not enter S phase, without reduction of cyclin D1 levels. In contrast, breast cancer cells were resistant and not arrested by the same doses of Caly A. This led us to investigate the basis for the effects of Caly A and we found a differential effect on serum-stimulated calcium channels in the Hs-68 fibroblasts
To analyze effects on cell proliferation we applied extremely low doses of Caly A (0.3 and 1.0 nM) that did not cause loss of cell adhesion or cell shape changes. To monitor proliferation we incubated cells with the thymidine analog bromodeoxyuridine (BrdU) and analyzed its incorporation into DNA by immunofluorescent staining. The concentration of BrdU and the duration of labeling were selected to maximize the fraction of BrdU positive cells [
Effects of Caly A on BrdU incorporation in human breast cancer and non-cancer cells. (
The fraction of cells that incorporated BrdU into DNA under control conditions was different among these cell lines, presumably reflecting differences in their cell cycles. All three cell lines showed no effects of this long term incubation with 0.3 nM Caly A and the results were independently replicated. This resistance to Caly A was unexpected and for a comparison we carried out the same assay using two other human cell lines, both with normal karyotype: retinal epithelial ARPE19 cells and Hs-68 fibroblasts. In contrast to the breast cancer cell lines both of these non-cancer cell lines showed a much smaller fraction of BrdU positive cells under the same conditions (
Effects of Caly A on cell cycle progression in breast cancer and non-cancer cell lines. For each cell line and condition 4 images were chosen and the number of total and BrdU positive cells were counted. The percentage of cells that incorporated BrdU was calculated for Calyculin A treated cells and controls. Plotted average value ± standard error, with
Since Caly A blocked progression into S phase, we predicted that Hs-68 fibroblast and ARPE19 epithelial cells would show reduced levels of endogenous cyclin D1. We employed double immunofluorescent labeling to assay for the levels of cyclin D1 and BrdU in the same individual cells (
Effects of Caly A on cyclin D1 levels. (
We carried out a series of control experiments to validate the specificity of cyclin D1 immunostaining. Human Hs-68 fibroblasts were incubated with or without FBS and processed using the identical staining protocol (
The effects of low dose Caly A on expression of cyclin D1 were examined in human fibroblasts, epithelial cells and two different human breast cancer cell lines, MDA-MB-231 and MDA-MB-468 (
Effects of Caly A on cyclin D1 levels analyzed by Western blotting. Breast cancer and non-cancer cell lines were treated with indicated doses of Caly A for 45 h and processed for immunoblotting with cyclin D1 antibodies. Staining for actin is shown as the loading control. Data are representative of 3 independent experiments.
The extended treatment of the cells with Caly A had no noticeable effect on the levels of cyclin D1 in any of the cell lines, except for ARPE19 epithelial cells that showed some reduction in D1 at the higher dose of Caly A (
These results raised questions: how did 0.3 nM Caly A produce G1 arrest in the cell cycle without reduction of cyclin D1, and why no response in breast cancer cells? We tested whether the low doses of Caly A over an extended period of time would affect the phosphorylation of endogenous proteins (
Caly A effects on protein phosphorylation. Non cancer (
Similar results (
What is targeted by subnanomolar doses of Caly A to prevent progression into S phase? This must be different in breast cancer cells compared to the non-cancer cell lines we used as controls (Hs-68 and ARPE19). We calculated that the effects were being produced by about 3 × 106 molecules of Caly A per cell, a relatively low number. Some of the earliest publications describing the actions of Caly A reported effects on intracellular calcium levels [
Caly A effects on serum-induced calcium influx
When MDA-MB-468 breast cancer cells were stimulated by serum following overnight deprivation there was a sustained increase in intracellular calcium (
In this study we discovered that calyculin A at subnanomolar concentrations prevents G1 to S-phase progression of human Hs-68 fibroblasts and ARPE19 human epithelial cells, but not human breast cancer MDA-MB-468 or MDA-MB-231 or MCF7 cells. All the cells tolerated treatment with low dose (<1 nM) Caly A for an extended period of time (2–3 days) with no loss of viability and with no noticeable shape changes that are hallmarks of Caly A inhibition of PPP phosphatases. Our original intention was to examine effects of low dose Caly A on G1 to S phase progression and correlate that to reduced levels of cyclin D1. We previously had found that nanomolar levels of Caly A increased cyclin D1 phosphorylation at T286, triggering degradation via the proteasome [
Early studies found Caly A at concentrations ranging from 10 nM–1 μM caused contraction in the smooth muscles of guinea pig (taenia coli or taenia caeci) and rat aorta in the presence or absence of external calcium [
We propose that non-selective cation channels (NSCC) could be the novel putative target for low dose Caly A. Our experiments showed no increase in intracellular calcium concentration with Hs-68 fibroblast when potassium chloride up to 120 mM was applied on cells to induce depolarization. We have attributed this to the absence or low expression of voltage-gated calcium channels in these cells. Several studies have investigated ion channel activation by serum [
MDA-MB-231 and MDA-MB-468 breast tumor cell lines were maintained in L15 media (Gibco 11415, Grand Island, NY, USA) containing 10% FBS at 37 °C in non-CO2 (air) conditions. All the other cell lines were cultured in a humidified 5% CO2 atmosphere at 37 °C. MCF7 breast cancer cells were grown in MEM (Gibco 11095, Grand Island, NY, USA) supplemented with 10% FBS, 10 μg/mL human insulin (Sigma 051M8418 St. Louis, MO, USA), 1 mM non-essential amino acids (Gibco 11140, Grand Island, NY, USA) and 1 mM sodium pyruvate (Gibco 11360, Grand Island, NY, USA). Hs-68 cells were cultured in DMEM high-glucose (Gibco 11965, Grand Island, NY, USA) with 10% FBS. All cells were passaged every 2–3 days using 1× PBS (Gibco 14190, Grand Island, NY, USA) and 0.05% trypsin-EDTA (Gibco 15400, Grand Island, NY, USA) and were grown to the maximum number of 10 passages. The following antibodies were used to perform western blotting experiments: anti-cyclin D1 (Cell Signaling Technology 2926S, Danvers, MA USA), anti-phospho44/42MAP Kinase Thr 202/Tyr204 (Cell Signaling Technology 9101S, Danvers, MA USA), anti-phosphoS6ribosomal protein Ser 240/244 (Cell Signaling Technology 2215S, Danvers, MA USA), anti-phosphoRb S807/811 (Cell Signaling Technology 9308S, Danvers, MA USA), anti-p107 (Thermo Scientific MA1-12362, Suwanee, GA, USA), anti-phospho-Akt1 Ser 473 (Upstate Cell Signaling Solutions 05-736, Lake Placid, NY, USA), anti-actin (Sigma A2103 St. Louis, MO, USA).
The experiments were performed using protein coated 12 mm glass coverslips (Fisher Scientific 12-545-80, Suwanee, GA, USA), situated in wells on 24-well plate. Wells with coverslips were prepared in advance by adding 400 µL/well of fibronectin (Sigma F0895, St. Louis, MO, USA) diluted in PBS 10ug/mL, followed by incubation for 2 h at room temperature (RT) or overnight at 4 °C. Coverslips were washed 3× with PBS. Cells before plating (2.5 × 104 cells/well) were mixed thoroughly in growth medium by vigorous pipetting at least 10 times in order to obtain single cell suspension. Next day Calyculin A (Sigma C7632, St. Louis, MO, USA) was diluted in medium and added to appropriate wells. To evaluate cell proliferation, 24 h before fixing cells BrdU labeling reagent (Invitrogen 00-0103, Grand Island, NY, USA) was added according to provider's instructions to final concentration of 100 μM. After that time cells were washed with PBS and fixed in 3.7% formaldehyde/PBS for 15 min. After 3× PBS wash cells were permeabilized by 10 min incubation in 0.1% Triton X-100 (Fisher Scientific BP151, Suwanee, GA, USA). After 3× PBS wash, DNA denaturation was performed by coverslips incubation in 2M HCl for 20min, followed by neutralization using 0.1 M sodium borate pH 8.5 (2 min). Coverslips were blocked for 1 h with 2% BSA, 5% normal goat serum (NGS) in PBS. Mouse anti-BrdU Antibody (Ab) (Invitrogen B35128, Grand Island, NY, USA) and rabbit anti-cyclin D1 Ab (Cell Signaling Technology 2978, Danvers, MA, USA) were diluted 1:100 in 2% BSA-PBS solution and incubated overnight in 4 °C. Secondary Abs-goat anti-mouse AlexaFluor 488 (Invitrogen A11001, Grand Island, NY, USA) and donkey anti-rabbit AlexaFluor 594 (Invitrogen A21207, Grand Island, NY, USA) were diluted 1:1000 in 2% BSA/PBS and incubated for 2 h in RT. Hoechst 33342 was incubated for 15min at 1:10000. After each step fixed cells were washed 3× in 0.1% BSA/PBS.
Samples were examined and wide field images were obtained using immunofluorescence Nikon Eclipse E800 microscope equipped with a Hamamatsu 3580 camera and 20X/0.50 magnification objective (no oil immersion). OpenLab 5.5.1 Software was used for image acquisition, processing and analysis.
Cells were plated (depending from cell line) 1.5–3 × 105 cells/well in 6-well plate. After 45 h cells were washed with PBS and whole cell lysates were prepared by applying 1× Loading Buffer (diluted from 4× stock: Tris-HCl pH 6.8, 0.25 M, SDS 8%, glycerol 30%, β-mercaptoethanol 10%, bromophenol blue 0.2%, protease inhibitor cocktail) directly to the cells after washing with PBS, followed by 10 min incubation at 100 °C. Protein samples were resolved using SDS-PAGE on precast gels from BioRad (567-1123, 567-1023 Hercules, CA, USA). Proteins were transferred from gels to nitrocellulose membranes by electrophoresis for 80 min at 25V using a semi-dry transfer apparatus (Transfer Buffer: Tris 25 mM, Glycine 192 mM, methanol 20%). Membranes were blocked with 5% bovine serum albumin (Sigma A7096, St. Louis, MO, USA) dissolved in Wash Buffer (Tris-buffered saline with 0.1% Triton X-100). Membranes were probed overnight at 4 °C with primary antibodies and washed 3× in Wash Buffer. Primary antibodies were detected using fluorescent secondary antibodies donkey anti-rabbit IR Dye 800CW (LI-COR 926-32213) and goat anti-mouse AlexaFluor 680 (Invitrogen A21058, Grand Island, NY, USA). Western blot images were acquired on an Odyssey Infrared Imaging System and software (Licor Biosciences, Lincoln, NE, USA). In each case the amount of protein loaded per well was determined to obtain the same actin signal quantified using Odyssey software. All western blot experiments were performed multiple times with similar results.
Cells plated onto 25 mm coverslip culture dishes (Fisher Scientific 12-545-102, Suwanee, GA, USA) situated in wells in 6-well plate (Hs-68 2 × 105 cells/well; MDA-MB-468 4 × 105cells/well) were grown under standard conditions. Next day media was replaced for serum-free media and cells were subjected to overnight starvation. Prior to experiments coverslips with cells were transferred to imaging chamber, loaded with 6 μM Fluo-3, AM (Invitrogen F1242, Grand Island, NY, USA)-fluorescent intracellular calcium indicator and incubated for 30 min in 37 °C. After that time the excess dye was washed off and cells were covered with DMEM high glucose phenol red free media (Gibco 21063, Grand Island, NY, USA) containing 5 mM CaCl2 with or without 0.3 nM Caly A. Imaging was carried out on a Leica SP5 X microscope system equipped with a 63X/1.2NA water objective. The excitation 500 nm was selected from the white light laser module and the emission signals of 510~570 nm (set through the acoustic optical beam splitter) were measured by a photomultiplier tube. Time-lapse images were taken every 2 s for 4 minutes. The treatment with serum to final concentration of 20% was typically applied at 60 s. Data acquisition and analysis were performed using the Leica LAS AF software. All experiments were performed at least 4 times, in each experiment 15–30 cells were recorded and analyzed as Regions of Interest (ROIs).
We propose that Caly A arrests cell proliferation because it blocks serum-stimulated calcium uptake by inhibiting non-selective cation channels. In contrast, human breast cancer cell proliferation is not blocked because calcium enters these cells by other channels. Our results suggest that Caly A is a dual action toxin that acts as a channel blocker at subnanomolar doses, in addition to its well established effects as a PPP phosphatase inhibitor at higher doses.
The authors thank the University of Virginia Keck Center for Cellular Imaging, in particular Ammasi Periasamy, Vinod Jyotikumar and Yuansheng Sun for assistance in recording intracellular calcium levels. Zygmunt Derewenda arranged for a visiting research position and the project was supported by USPHS grant CA142823 from the National Cancer Institute.
The authors declare no conflict of interest.


text:
Mark Foster, Kimbra + A-Trak Duke It Out at Mexican Wrestling Match in 'Warrior' Video
Converse has released yet another all-star collaboration, and this time, their trio matches up Mark Foster of Foster the People, 'Somebody That I Used to Know' songstress Kimbra, and electro DJ A-Trak. The 'Pumped Up Kicks' vocalist, the 'Cameo Lover' indie pop darling, and the hip-hop / house hitmaker all worked on the track 'Warrior,' the video for which recently premiered on YouTube.
In the clip, Kimbra, Foster and A-Trak are enslaved in an underground Mexican wrestling ring. With her hands bound, Kimbra is forced to sing as the frontwoman of a mariachi band, costume and all. Meanwhile, Mark Foster and A-Trak are manhandled until they compete as luchadores in the ring. As the video progresses, Kimbra stands by idly, singing with a longing look in her eye as the corrupt head honcho takes bets while Foster and A-Trak get their a–es beat.
However, the tables quickly turn as Foster and A-Trak join together to create an unstoppable tag team, clothes lining their way to freedom. They eventually take down the greedy overweight ring leader, but it's it's the gorgeous raven-haired Kimbra who finished the job, round-housing that sucker practically into space, all the while looking adorable in her purple kicks and unitard. Liberation at last!
The 22-year-old singer-songwriter, who is getting ready to release her debut album in May and is making waves with her feature on Gotye's 'Somebody That I Used to Know,' actually wrote about the song on her blog:
Mark's initial suggestion after writing the 'pushing me down' hook was that we try and write the song about something or someone who threatens to take away our joy. There are people who always want to bring you down, and 'Warrior' is about standing up for what you believe and following your convictions even when people want to prove you wrong or explain away the mystical or extraordinary experiences you have… This song is a call back to embrace that child-like wonder and strength in 'feeling' things, while not always fully understanding them. There's a war-cry at the heart of this song that I feel strongly about…. Will we raise our men and women to be strong in heart & 'to cry for the ones they love'? Or just automated to be strong in body and mind, but lacking in empathy? Hold onto your light…. follow it to the end… and fight the man! Preferably with a roundhouse kick to the face in a Luchadore mask and spandex leotard!!! Haha ; )
What do you think of 'Warrior'? Does this trio make sweet, beautiful tunes together? Check out the awesome video below and leave us a comment afterwards.
Watch Mark Foster, Kimbra and A-Trak's 'Warrior' Video


text:
SHERLOC Mobile
A to Z mysteries. Books W-Z [cd] / Ron Roy. - SHERLOC
A to Z mysteries. Books W-Z [cd] / Ron Roy.
Other title:
X'ed-out x-ray.
Yellow yacht.
Zombie zone.
New York : Random House/Listening Library, p2011.
Library ed.
9780307915658 :
0307915654 :
Call number:
jCD E ROY
Physical description:
4 audio discs (4 hr., 2 min.) : digital ; 4 3/4 in.
Wolves -- Juvenile fiction.
Thieves -- Juvenile fiction.
Zombies -- Juvenile fiction.
Children's audiobooks.
Compact discs.
Read by David Pittu.
Summary note:
The white wolf: While visiting their friend Wallis and her adopted daughter on Mount Desert Island, Maine, Josh, Dink, and Ruth Rose investigate why three white wolf cubs were stolen, and by whom.
The x'ed-out x-ray: Ruth Rose, Josh, and Dink attend a concert during which someone steals the star's diamond pendant, and they are soon on the trail of the thief.
The yellow yacht: Dink, Josh, and Ruth Rose help catch the thieves who have stolen gold from Sammi's parents, the king and queen of Costra.
The zombie zone: Reports of zombies and grave-robbers alarm the people of a Louisiana swampland village, but Ruth Rose, Josh, and Dink begin to suspect that the supernatural may not be the cause of the eerie occurrences.
Contents note:
The white wolf -- The X'ed-out x-ray -- The yellow yacht -- The zombie zone.
Item availability
College Avenue Library
jCD E ROY
Checked out
Franklin Road Library
jCD E ROY
Sent to Library Service Center


text:
|Thread Tools
We bought this one (with a 20% off coupon)
and we love it. I can put her down on the floor now! And she likes the floor more than the bouncy seat. So I've taken to putting her down on the mat when I get home from work while I let the dog out and put milk in the fridge.
It stays together well so we can move it from the living room to her bedroom with no problem.
Pet-mom to Squirt with FLUTD & Maya the deaf wonder dog .
mpp, thanks for what you said. i'm so thankful that good medical care is there when we need it.
ericka, i feel the same way about our NICU nurses and docs. though i do think we could have gone home earlier... i think some levels (hematocrit, bilirubin) were lower by the time we left than what would have been grounds for admission, and everything else was resolved within a couple of days of charlie's being admitted. i'd love to talk to someone who knows about the use and weaning off of intravenous nutrition in the NICU too.
lnf, wow, what a life you've had! i can't imagine having your experiences, and it's part of makes this DDC so fun.
s to those of you struggling w/ getting enough rest and your babies sleeping. jsh, i'm with you on working with a plan without it being consuming/frustrating. i'm kinda all or nothing...
me+him for 15 yrs, welcomed our little one march 25th, 2010.
me+him for 15 yrs, welcomed our little one march 25th, 2010.
no circ/vax-babywearer-cosleeping-pumping/breastfeeding-homeschooling single mama to Junebug
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, Sugarbug
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, booboo
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and TTC
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I was just scrolling through some older posts and I wonder what happened to a lot of the moms who were in our DDC
What happened to Sehbub? She used to post a lot, and now she hasn't posted in a really long time. I hope all those moms are just busy, and everything is going really well.
Pet-mom to Squirt with FLUTD & Maya the deaf wonder dog .
so dani has her first official cold. her DCP noticed a sniffles and cough wednesday, then a fever Wedn night, then nore snot and cough today. poor kid. so DH played SAHD and took her to campus with him and to all his meetings. what a good husband. Then I had a migraine so i came hone from work early and fell asleep with Dani and DH cleaned the whole apt and did laundry!!!! OMG. (I bet its unfolded somewhere and not put away LOL). And my migraine is still horrible, so he made dinner and now he just took the kids to the playground! I LOVE him.
A PhD = + +
Jen, homeschooling geek of DD 9 and DS 6.
I got pregnant accidentally (on Halloween, dressed as a girl from the 80's complete with sideways pony tail!) I was 19 and in college. DH is the same age also. I ended up going to classes with a nursing infant and doing online classes, but then I dropped out with only two semesters left because I just...didn't care. I was so done with college and knew I wanted to stay home, so figured I should just forget it and not have the stress of the classes.
The transition to motherhood was fairly easy for me, but DH struggled to figure out how to stop being a kid and instead be a father. We've never had a point in our marriage where we've been alone though, so we have nothing else to compare it to.
I'm 24 now, by the way.
We both want a lot of kids too, spread out over 10-20 years. We haven't picked a set number. We both think we'll know when we're done. Or as DH tells people, we'll stop when it's not fun anymore. Our only stipulation is that we have to be able to afford to care for their basic needs, and I don't want to get pregnant before each baby has nursed plenty. I want each baby to have their own special babyhood, you know? I'm very excited about having a large family and I love that DH feels the same way.
I am so glad we started our family early, on purpose or not, and I wouldn't want it any other way. People (family mainly) seem to think we should have regrets, having a baby so early and not having time alone as a couple and not having our 20's to party. But I look around at my old friends, some of who are still partying and dating, and I am so happy that's not me. SO HAPPY. If I had to do it over I wouldn't change a single thing.
Momma to Sweet Rosie 7/06, Lost Baby J 1/09 at 12 weeks pregnant, Spitfire Ada born 4/21/10, and Baby Boy due July/August 2013!
Aspiring urban homesteader, photographer, homeschooling momma! Blog link in my profile.
And just wait until you are a couple further kids down the line. I don't know of any baby I've ever seen who is more loved on than Mercy is. She literally has a line of siblings every single day, taking turns kissing her. A large family, when everyone involved is both equipped for it and wants it, is a wonderful thing.
Seeing how much fun you guys are having (and actually having a normal-needs baby) makes me slightly less sure I'm done. SLIGHTLY.
Jen, homeschooling geek of DD 9 and DS 6.
I second the idea of the tiles, we have them (but got them for the dog) and they are great. We got ours at Costco.
The thing that annoys me the most about the medical establishment is that I now feel like I can't trust them. I want to, but I've heard too many stories, and been through several issues myself. They are amazing when they are needed, but they need to step back and be better about deciding when they're needed. Im not sure how T would have been born without the epidural, which was an eye-opener for me. I was sure I'd never need one.
T fell asleep in my lap after nursing for the first time in over a month, such cuteness. I wish I'd listened when people told me I'd look back fondly on those days I Was "trapped" on the couch when he fell asleep on me. <sigh>
He was up every 45 min again last night, but so far tonight he's been down for almost 3 hours. Phew...
Oh, and our Sophie arrived today! T seemed to like it the little time he had it before bed. I'm excited!
We did want a bunch of kids but I think we're done at 4, because I don't handle pregnancy well and we're kind of sick of moving through life in fits and starts. Time for the next part of life, you know?
Mama, homeschooler, midwife. DD (13yo), DS (11yo), DD (8yo), DD (3yo), somebody new coming in November 2013.
Pepper, we seem to be in the same boat. I may not have gotten pregnant while dressed like a girl from the 80s, but I did skip my senior prom to hit the justice of the peace instead, at roughly 20 weeks pregnant with our oldest. Oops. But what I want to know is HOW DID YOU BEAT ME TO HAVING POULTRY!?!?
And just wait until you are a couple further kids down the line. I don't know of any baby I've ever seen who is more loved on than Mercy is. She literally has a line of siblings every single day, taking turns kissing her. A large family, when everyone involved is both equipped for it and wants it, is a wonderful thing.
We used to live with my in-laws. One day I couldn't stand it a second longer, and as luck would have it we found a semi-crooked mortgage broker who fudged our income quite a bit and got us a mortgage for $100,000. So we bought the first house that didn't have fire damage or rats or bats. Then, I got some chickens. And that's how I beat you in owning poultry.
Side note: I do not recommend buying the first livable house you come across in your price range. We have since purchased a new roof, a new water main (OMG.), new fixtures, and our electric wiring has proven scary. Also, our yard has no real grass...only weeds and mud patches. But I own this house and my in-laws don't live here. FTW!
Momma to Sweet Rosie 7/06, Lost Baby J 1/09 at 12 weeks pregnant, Spitfire Ada born 4/21/10, and Baby Boy due July/August 2013!
Aspiring urban homesteader, photographer, homeschooling momma! Blog link in my profile.
Well, a duck in the tub *could* be effective in terms of keeping me from having another accidental water birth. I'm too high a risk profile for water birth, so maybe the duck would be a good addition to the family? And God help him if my 2 year old ever got a hold of him.... It might be a case of "who's quacking now!?!?"
We started early (21) as well, and are obviously digging the kid/baby thing (3 in 4 years, ). I am really not sure we are done - logically I feel like we should be, and OH I am not ready for a fourth right now, but I find myself looking for 'the other one' when rounding up the kids, and just get the feeling there is someone waiting for us to be ready (or to be caught off guard...). When i got pregnant with Becky, i really thought she was the little boy I had been sensing, but she is not that person. She is exactly who i need right now, but she was not who i was expecting. Give me a couple years, and i may be willing to meet this little person. Also, I don't have that "next phase please!" feeling yet. I try not to think about the fact that becky could be our last, because I'm not ready for this part of my life to be over.
My chickens laid their first eggs today! Er.. yesterday, or maybe the day before. But i found them today! We just threw out our eggs because of the egg recall, so I think the chickens felt sorry for us.
We didn't start that young (dh was 23 and I was 27) but we didn't have much time a lone as a couple. I used to hate telling people but now that we've been together for 11 years I don't feel so bad! I was pregnant within six weeks of meeting him and we got married 3 months later. I never thought I'd believe in love at first sight but it was pretty much like that for us. I still feel that way about him. He's amazing and I can't believe I found him. Of course we kept having kids We also did the coparenting thing while we did grad school (started when Piper was 3 months). Our life together has always included the beasties.
Sometimes I wish we had started younger but then I don't regret what I did when I was younger either. I had a good time and was ready to settle down when I meet dh. Some of that yearning is because I know we'll only have one more. As it I'll be 41 when having the baby. But on the other hand, five seems plenty and that number resounds with me for some reason. I keep thinking we should stop but I feel like we have one more. I'll be hopefully writing my dissertation with that one...
Oh and I ordered my birthday present! I got a SweetPea Ring sling. I'm really hoping it will keep my little R happy. She's been so fussy lately, and it's hard for me to get anything done in terms of homeschooling and cleaning.
And...I have to TEACH my first class with her!!!! H has open house at this school so I have to take her. I'm so not happy about this. I'm only going over the syllabus and showing a movie so I hope she's good.
Mama to Umberto 12, Camille, 9, Piper 7, Rowena 2, and Jude Therese Prenatal DS diagnosis due December 23.
Jen, homeschooling geek of DD 9 and DS 6.
my husband and i met at 18 and waited til we were 23 to get hitched, then we waited 11 yrs (eta: oops, 12) to get pregnant. we had a lot of fun and moved a lot, and now we're back near family and loving experiencing this new stage of life together. i don't have a single regret on timing either, though i recognize the advantages and disadvantages of our choices.
decided to put off charlie's next WBV to 6 months, and focus on reading up on vaccine info. other than getting a shot, i don't feel like he needs to see a dr. right now, and i just need to feel more sure about what we're doing before i start the road of vaccinating him.
meanwhile, in my local news... http://www.citizen-times.com/article...NEWS/308200041
me+him for 15 yrs, welcomed our little one march 25th, 2010.
DH and I were high school sweethearts, got married when I was in college, then waited 6 years for kids (until our late 20s). I'm happy with our timing -- we moved to the other side of the country, I worked an intense but amazing job that allowed us to save up, and DH got training and fabulous experience in his career. We were able to take what we accomplished in CA, move back near family, and be in a place were I can stay home and DH works a job that lets him be home all week.
We traded off moving away from our friends and into an area of the country where it's hard to find like-minded people, so it's been an adjustment. But there's not much I wouldn't give up to have this much time together as a family.
Jen, homeschooling geek of DD 9 and DS 6.
He rolls back-to-front easily when he's awake, but not when he's sleeping. He rolls front-to-back easily when sleeping, but not awake.
Jen, homeschooling geek of DD 9 and DS 6.
I think I just broke my lawn mower. What does it mean when the pull cord won't pull anymore? I still have half the front lawn to go and I can't pull the cord to start the motor. ARGH! Stupid pieces of sh*t lawn mower is from 1989, seriously. One downside to starting a family early is being poor and having to use old stuff. lol.
Momma to Sweet Rosie 7/06, Lost Baby J 1/09 at 12 weeks pregnant, Spitfire Ada born 4/21/10, and Baby Boy due July/August 2013!
Aspiring urban homesteader, photographer, homeschooling momma! Blog link in my profile.
Vaccinations: S was fine with her shots the other day, and I've done enough reading and thinking about them to feel good about doing them... but while we were waiting, DH turned to me and said, "this isn't going to give her autism, right?". It was kind of a "joke" but it made me so mad that he was just tossing off a "are we doing the right thing?" comment when he has done basically nothing to research or talk about it, so the decision feels mostly like it's mine. Argh!
Rolling: still nothing! She hasn't rolled from front to back in at least a week, and not from back to front ever. Everything else is on track and she sure is wiggly, so I try not to think about it. But I am curious... When she's on her tummy, she seems like she wants to crawl.
Sleeping: we reinstated a strict bedtime routine (which means I am the jerk that is kicking visitors out, shushing my husband from trying to play with the baby after her pajamas are on, etc) and last night it seemed to help a lot! She was back to falling asleep at 8:30 pretty easily, woke up once at 9:15 but fell back asleep, and slept peacefully in her crib till 2:30 . Then she nursed (was obviously way hungry), fell back asleep till 5:30, woke up to wiggle around and talk to herself. I tried to see if she'd fall back to sleep by herself, and I think she almost did, but after a half hour or so of chattering, she started fussing and I brought her into bed with us. Then she slept until 8ish, woke up, and fell back asleep until 9:45. AWESOME!
(On the flip side, I'm exhausted... Somehow when she stays up later, I can fall right to sleep. When she goes to bed on the early side, suddenly I have all of this energy because I'm like "time to myself?!!")
I'm also experimenting with seeing if she'll do one morning nap, and then hold out for a longer afternoon nap. This means I just get in bed with her around 3 and stay in there nursing or having quiet time until 5ish. I'm feeling good about the routine... so I'm sure that means it'll change at any moment
I have to keep cheerleading for us because I just read on FB that a friend's 8 week old son is already sleeping through the night, and another friend with a 3 month old is telling me "they don't NEED to eat at night, they just want to be with you" even though I know for sure S is really hungry in the middle of the night. Plus anything to keep my period at bay is fine with me
We started dating at the very end of his junior year, my 4th year of 5. I'm three months older than him to the day, and his 29th birthday was yesterday.
Pet-mom to Squirt with FLUTD & Maya the deaf wonder dog .
AFM -- Probably because of that 2 hours of rolling practice last night, Sprout has taken 3 - 1.5 hour naps today! I have no idea what to do with myself. After an hour, I get all antsy and worried about how long he's sleeping. Now, we'll see if he'll sleep tonight or not.....
Jen, homeschooling geek of DD 9 and DS 6.
Posted via Mobile Device
sleep - she's waking up more, but some of that is loud DH and some of it is teeth. she's so close to poking that canine through!
vax - we just got our first vax last week since all of us are back at school. I won't do more than 2 shots at a time, so we just did the most important for daycare threats, DTaP and Prevnar. Jess, I can relate with DH not looking into vax stuff. pissed me off too because he didn't resarch ciric that much with my son, and I wonder if he had made a diff decision if he had taken the time.
chickens -DH wants some, but I think they are too much work. We can't keep up on laundry and cleaning, how are we going to do chickens? besides, now we have apt life so chickens wouldn't be approved LOL but this egg recall does make me want some!
rolling - she's done it a couple times, but she's really more into her johnny jump-up than any back or belly time.
pepper - how the heck do you find time to mow a lawn?
work - finished 1 syllabus, still have 1 to go, but no book in sight. i still need a chair and a whiteboard too. ugh. how can i start work w/out the things I need? grrr... still have some diss stuff to do, but had a severe mirgraine 2x this week including this afternoon. I went and got my amalgram fillings replaced with composite today and that's what started this one. ouch...
A PhD = + +
i would actually like to have 2 more but BF is snipped (and wants to stay that way) so i guess that's the end! makes me very very sad. but also i am trying to tell myself that its time to learn something NEW. i've been MOM for 6 yrs and only that, its time to move on. 5 kids always resonated with me too! ironically (or not?) BF has 2 boys so that makes 5.....
i am exhausted of thinking about sleep...just can't seem to get enough.
made the lactation cookies yesterday. i doubled the recipe (!!!!) and made them huge. i also added in wheat germ and raisins. i call my multivitamin i can EAT. YUMMY!!!!
very SHORT naps today...wth?
Plus, I don't mind waking up once to nurse her in the middle of the night - I can stay mostly asleep, it's a nice way to make sure she's alright (since we dont' cosleep) and connect. It's the post-5am wiggling that kills me.
I have to say, I thought my spontaneous letdowns would have slowed by now. I still have my milk let down 5+ times a day out of nowhere... I shouldn't complain (I am grateful for the supply, and it's helping me lose weight quickly) but it's kind of weird. Sometimes it's obvious (when I was bending over a giggly baby this morning, or last night when I was thinking about her crying when getting her shots, or often in the shower) but sometimes it's totally random. Maybe it will slow when she starts eating food too? I'm just surprised, I never knew it would be this frequent.
Also, my left boob is way bigger and produces way more milk. I actually thought DH's eyes were going to pop out of his head the other day when I nursed the right one to completely emptiness and the left was all full.... had to be at least 2 cups' difference! Will I always be unbalanced, even when done nursing?!
Jen, homeschooling geek of DD 9 and DS 6.
About the sleeping through the night thing. If I let L sleep on me all night she sleeps through the night. BUT, that doesn't mean she doesn't eat. Homegirl can nurse in her sleep. She can throw her body from being on her back sleeping to being on her side or almost stomach to eat. She sleeps with her upper body on my upper stomach and her lower body on the bed. Same general position as the nap nanny puts her. If I move her over to the nap nanny I get about 2-5 hours before she wakes up. But even then, she falls right back asleep when I get her and nurse her. To me it's really basically sleeping through the night. I consider most anything where I don't have to get out of bed as STTN.
I just went to my work picnic for the new students. The second years were invited, too. I sat down with L and one of the second years came up to see her and was like "fuzzibunz! that's what we use!" and went to get his wife to show her. We discussed cloth diapers for a minute and then just chatted about babies (their kid is about 9 months old). I nursed L and she said that she feels like she's the only one who ever nurses in public and she thanked me profusely for doing it, too. THEN I found out she lives in my neighborhood and hosts mom groups! Super excited.
Pet-mom to Squirt with FLUTD & Maya the deaf wonder dog .
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text:
Description of spotted wing drosophila
The spotted wing drosophila (Drosophila suzukii) is a small invasive fly that looks very similar to our usual fruit fly (sometimes called vinegar fly) but it acts very differently. The spotted wing drosophila will attack undamaged fruit. It is especially damaging to thin skinned fruit like raspberries, blackberries, blueberries, cherries, strawberries, grapes, etc. It attacks right as fruit are ready for harvest so control measures with insecticides are limited.
Spotted wing drosophila, native to Asia, was found in the mainland U.S. (California) in 2008, by 2009 it was found in Oregon, Washington and Florida. By 2010 it had spread to several other states including Michigan. In 2011, Wisconsin confirmed SWD and in 2012 Illinois and Minnesota confirmed SWD. It appears that SWD is here to stay and has spread very quickly. In Iowa we have collected adult flies in a trap in Story County.
Life cycle of spotted wing drosophila
Spotted wing drosophila can be distinguished from the normal fruit fly (Drosophila melanogaster) but the presence of spots on the wings of the male and a serrated edge on the ovipositor (egg layer) of the female. It is the serrated, saw-like, edge on the female's ovipositor that allows this species to attack pre-harvest healthy fruit.
Management of spotted wing drosophila
Sanitation is necessary for management of SWD. Picking all fruit when harvesting and remove and destroy any fallen, damaged and overripe fruit. There are insecticides available but options will be limited. At this point there is no need for treatment until SWD has been confirmed in your area.


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Football Contest
Pasta primavera brings thoughts of spring
By Iris Clark Neumann
Tuesday, March 26, 2013 4:52 AM
Pasta Primavera
OK, enough snow! I want spring now!
With a portable computer and Internet in a motel room I can even write columns away from home. I cooked before I left, creating a second batch of pasta primavera so I could take a photo, but had been disappointed not to find the same really nice asparagus I had in my first batch. I had clipped most of the spare basil off my plants that are growing under lights, so the second batch wasn't quite as tasty either.
I substituted broccoli for the asparagus. I like the recipe because it creates a simple sauce for whole wheat pasta and combines a nice group of veggies. It's a vegetarian recipe, but cooked meat can be added.
I am away from home this week for the annual Minnesota Shade Tree Short Course. This is an amazing annual conference dedicated to trees, tree care and tree culture. I still haven't decided which classes I am choosing; it's such a hard choice when all sound so interesting. But I need certain courses for re-certifications and others will be helpful for my job.
It snowed back home today, snowed until mid-afternoon, so I was concerned the drive up to Roseville might be challenging. It turned out they had NO snow up here, according to my son, Doron, whom I chatted with on Facebook. I am stopping over to see his daughters tomorrow evening after classes and a supper at the Como Park golf course.
My husband and I are headed back up north this coming weekend and I finally ordered a pair of snowshoes that I am hoping will have been delivered to my house by the time I get back home. We might as well enjoy the snow while it is still here.
Otherwise, I am thinking spring. My lower level is swimming in artificial light with a variety of garden crops growing there - herbs and vegetables. Logan is getting ready for his upcoming season of vegetable gardening and I am enjoying the prospects of things to come. There's still lots of home-canned goods not yet consumed that will keep us eating until the new things start growing.
I admit I sort of went crazy with trying out various yellow tomato and green tomato chutneys, along with yellow tomato jams at the end of the season last year. I also canned a couple of cranberry sauces and cranberry jam, in addition to applesauce and apple butter.
The only problem has been finding ways of incorporating these things into my regular cooking. Logan suggests using some of the sauces on meats like pork chops. Cranberries and pork, that sounds good.
The next day
After one day of tree classes, I am filled with knowledge about global warming and how this can affect our tree populations in the years ahead. Although this winter has had plenty of snow, it is true as was pointed out by a speaker today, that we don't have the same sub-zero temps we had when I was young. Statistics of average temperatures over time also point this out.
I drove over to visit my granddaughters, Sylvie and Cora, after the dinner. It was a short visit, but it is always good to see them, and usually they are visitors at my house or up at the lake, instead of me visiting them at home.
They had been outside playing in the snow when I got there. We read "Madeline" and the Syd Hoff book, "Sammy the Seal." Imagine a talking seal who gets by with attending school, while on sabbatical from the zoo. In the end, he misses his food and water for swimming and heads back home. Madeline gets extra attention when she has an operation for removal of her appendix. Old stories, but they still have an appeal to them (well, at least to Grandma).
Now, for the recipe
Keep this one on hand for the new spring vegetables. I got an original version from an Olmsted County Health Services newsletter two years ago (their original recipe came from the Food Network). This is the same recipe I shared at the Eyota Farmers Market the last two years.
We are looking forward to another market season starting near the end of May. Something new this year, we will be honoring EBT/SNAP cards, thanks to a new grant through Blue Cross Blue Shield and the Minnesota Department of Health.
As mentioned above, I especially like pasta primavera made with asparagus and I have made it using dehydrated tomatoes instead of the fresh cherry tomatoes in the recipe.
During the height of tomato season, I cut cherry tomatoes in half, sprinkled them with a seasoning mix of dried basil, garlic powder and sea salt, after slipping out the seeds. I lined them up in dehydrator trays overnight and then bagged them into small zip-lock bags the next day after drying them all night. My husband enjoys eating these with a veggie dip.
Pasta Primavera
1-2 tablespoons olive oil
3 cloves minced garlic (or chopped green onions)
1 red bell pepper, seeded and cut into strips
1/2 pound thin asparagus, cut into 2 inch pieces (chopped broccoli can be substituted)
1 cup mushrooms (white or small portabellas), sliced
1 cup grape or cherry tomatoes, halved
1 cup chicken stock
1/2 cup low fat milk
1 tablespoon flour, dissolved in 3 tablespoons water
1/2 teaspoon each, salt & pepper
1-2 carrots, peeled and coarsely grated
5-6 ounces whole-wheat penne pasta or linguine
1/2 cup shredded or grated Parmesan cheese
2-3 tablespoons chopped fresh parsley
2 to 4 tablespoons shredded fresh basil leaves
Assemble the ingredients and cut up the vegetables while heating the water for the pasta. Start cooking the pasta in boiling water, according to the package directions while starting to cook the vegetables as described below. When cooked, reserve 1/2 cup liquid, then pour the pasta into a colander.
Heat oil in a saute pan over medium high heat. Cook garlic until soft, about one minute. Add peppers and cook 3 minutes, until they start softening. Add mushrooms, asparagus and tomatoes and cook about 5 minutes. Stir in the flour mixture and cook for a minute. Add chicken stock, milk, salt and pepper, and bring to a boil. Reduce to a simmer and cook until the liquid has thickened slightly, about 5 minutes. Stir in the coarsely shredded carrots. Toss the pasta with the vegetables and sauce. If needed, add the reserved pasta water.
To serve, sprinkle with Parmesan cheese, parsley and basil.
Note: Other seasonal vegetables can be substituted including pea pods, sliced chard stems, chopped chard leaves or chopped spinach, to name a few.
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Used Car Prices Expected to Fall in 2014: NADA
After five years of consistently rising prices, used cars should get cheaper this year according to economists from the National Automotive Dealers Association.
NADA analysts insisted that it's not a sign of a bursting bubble, however, but that used-car prices will simply get closer to their historical average. The average price of a used car rose by 18 percent between 2007 to 2013 and currently, used car prices are roughly 10 percent higher than the average price from the past couple decades.
SEE ALSO: Used Car Pre-Purchase Inspection: 10 Things to Check
According to Jonathan Banks, executive automotive analyst for NADA's used car guide, the elevated price of used cars is a "new normal," and the market remains in a solid position. NADA also predicted that 16.4-million new cars and light trucks will be sold or leased in the U.S. this year, up 6 percent. The used-car market should see roughly 42-million units sold worldwide.
[Source: Automotive News]


text:
SOUTH-AFRICA-EASTERN-CAPE-L ArchivesArchiver > SOUTH-AFRICA-EASTERN-CAPE > 2007-07 > 1184006198
From: "Becky Horne" <>
Subject: [ZA-EC] Newspaper cuttings from the Eastern Cape - Dynamite
Date: Mon, 9 Jul 2007 20:37:42 +0200
Hi Folks
Please join me in welcoming disp1? to the List. Hope you going to be very
happy with us. Here is a snippet of news from an Eastern Cape Newspaper,
which I hope makes you feel right at home.
Herald, 6 July 2007
100 Years Ago by Ivor Markman
A selection of interesting century-old snippets from The Herald of
News from week ending July 6, 1907.
Mail train runs into dynamite trucks
A railway collision occurred at Fourteen Streams last week which might have
had disastrous results. The mail train from Johannesburg to Cape Town ran
into a train consisting of trucks laden with dynamite and with a number of
empty carriages attached to the rear.
The latter were smashed, and as a result the traffic was considerably
delayed. Had the empty carriages not been attached to the trucks nothing
could have prevented a fearful explosion and probably great loss of life and
Best wishes
This thread:
|[ZA-EC] Newspaper cuttings from the Eastern Cape - Dynamite by "Becky Horne" <>


text:
Feature #15356
|Start date:
|Due date:
|% Done:
|Scripting: Problem
|Target version:
|Affected ArmA II version:
|First affected ArmA II version:
|Reproduced by another DH user:
|Single / Multi Player?:
|I am using some Mods:
|I am using:
|Reproducible for you:
|Related to content of DLC:
Add a new setPlayerRespawnTime command (to work in conjunction with the existing playerRespawnTime command).Implementation requirements:
- It simply changes the default respawnTime value, so it should be very easy to implement.
- I think it would only need to operate on the player's local client.
- It needs to be able to be set multiple times during the game (different value for each death).
- It's especially useful for PvP game modes.
- It should also be able to be changed even while the player is dead with the respawn timer already started.
- In some cases, the default time could be 30 seconds or even 600 seconds, which can then be adjusted to suit.
- The display of the timer can be controlled by the mission anyway, so the change won't be noticable.
- It would probably be best if the respawn time was reset to the default respawnDelay=X (set in description.ext), each time a player is respawned.
- If the player is dead and waiting to respawn, you could force an immediate respawn by setting setPlayerRespawnTime to zero
- For example, once a suitable spawn position is chosen.
- Another example use is to prevent a player spawning somewhere which has enemy waiting there to kill them upon spawning.
The benefit of this is to avoid having to create special safe holding areas on the map to spawn the players until they are ready to be moved into the proper mission areas due to additional mission controlled time delays and penalty times.
Eg: If using 'wave (group) respawn', or if using a respawn selection dialog, safe area spawning, penalty delay times, etc.
These special areas need all sorts of special checks: to prevent players entering and/or killing the spawned players while they're "waiting to spawn" or return to the proper area, scoring handling, ROE handling, etc all because there is no way to delay the spawning of a player.
All of this could be solved with a simple client-side value change. (This was requested 2 years ago on the old wiki wishlists.)
Related issues
Updated by kju over 6 years ago
- Due date set to 12/14/2010
- Status changed from New to Feedback
Same as #11192?
Updated by Dr_Eyeball over 6 years ago
Yes, looks like it's the same request. (Searched for playerRespawnTime and found nothing.)
Could you link this topic as a duplicate, for reference. It contains 2 points for consideration in the implementation.
You'll also have to start monitoring and crack down on the idiots who keep voting negative on these requests. It's getting out of hand.
Wish we could see who is voting in order to explain to them what it's intended purpose is. Seems people are of the mindset that if something is of no benefit to them directly, they comment against requests, rather than considering the overall benefits to the whole community.
Updated by Xeno over 6 years ago
I don't think that votes have a meaning at all.
As you can see I've requested the same like you 6 months ago and the chance that it will ever be implemented equals zero.
(And it can't be such a dramatic change as respawn time is a local client thing).
As long as it is not important for BIS we simply don't get it or maybe in a few years.
Beeing a little bit sarcastic here...
Instead we get features like playing a movie ingame in a resource which nobody ever asked for :(
Updated by kju over 6 years ago
- Due date deleted (
- Status changed from Feedback to Duplicate
Thanks. Merged your good report with the one from Xeno.
Overall people seem to use the voting sensible.
Also Xeno if you look into the addressed issues, apart from CTD,
most were among the highest votes ticket.
So it is simple not true that votes do not matter.
At the same BI foremost adds functionality they need for their plans.
Nothing new or non obvious. The marketing talk we all can understand and ignore.


text:
Some long-ago summer experiences can shock today's youth. As a child in Stoneham, Mass., in the 1950s and '60s, Neil Gussman rode his bike to Boston (about 10 miles) when he was 8. "When I told my kids that I rode to Boston one day, they could not conceive of doing something like that," says Mr. Gussman of Lancaster, Pa. "I think they suspected my mother of neglect." Noting that his four children are attending camps this year, he says, "My kids and I grew up on different planets."Read more in the Christian Science Monitor.
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With the resurgence of 3-D movies, theatergoers everywhere are stealing the complimentary glasses in an attempt to experience the fabled third dimension in real life. Gain a clearer perspective of the world with this Groupon.
$50 for $225 Toward a Complete Pair of Prescription Eyeglasses
Equip a corrective pair of Essential frames ($99.95+), or slip on a pair of designer eye enhancers such as those from Anne Klein and Vogue ($159.95+), or high-end frames from Versace ($239.95+) and Coach ($229.95+). Single-vision lenses adjust a singular field of vision ($119+), whereas specialty PearleThin complete lenses ($225+) are lightweight heavyweights.
Choose from eight Long Island Pearle Vision locations. Click here to see all eight locations.
Pearle Vision Long Island
Since Dr. Stanley Pearle opened the doors to the first Pearle Vision in 1961, the franchise has expanded to more than 800 stores nationwide. In these stores, optometrists assess the ocular health of patients before onsite opticians help them navigate the assortment of frames from brands such as Versace, Ray-Ban, and DKNY. If they're not in the store, clients can use the Try-On tool, uploading a photo to see what they or their dog looks like in different types of glasses. Pearle Vision also helps focus the world with contacts from Acuvue and Biofinity.


text:
Isaiah Washington has been in preliminary discussions with NBC about a deal, it has been reported.
There it is, after numbers of bad report Isaiah Washington is moving on. The actor, who's been fired from ABC's hit show "Grey's Anatomy" in early June, reportedly is in preliminary discussions with NBC about a deal on an undisclosed project.
Isaiah told Entertainment Weekly recently that he and his agent "are sorting through a ton of offers in both film and TV ... There is one network in particular that's very interested, but I don't want to say anything specific until it's a done deal."
NBC refused to comment on the much-talked negotiation though, while a representative for the star could not be reached for comment.
While the negotiation with NBC remained unconfirmed just yet, Isaiah publicist does confirm the actor is set to go on "Larry King Live" next Monday July 2nd to discuss his homophobic remark, rehab stint, and his firing from "Grey's Anatomy" with the intention to keep the public from hating him.
Isaiah has, in recent interview with the Houston Chronicle, revealed his plan to take legal action against "Grey's" producers, claiming "They fired the wrong guy. I have to clear my name."