text:
This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Single-cell studies of phenotypic heterogeneity reveal more information about pathogenic processes than conventional bulk-cell analysis methods. By enabling high-resolution structural and functional imaging, a single-cell three-dimensional (3D) imaging system can be used to study basic biological processes and to diagnose diseases such as cancer at an early stage. One mechanism that such systems apply to accomplish 3D imaging is rotation of a single cell about a fixed axis. However, many cell rotation mechanisms require intricate and tedious microfabrication, or fail to provide a suitable environment for living cells. To address these and related challenges, we applied numerical simulation methods to design new microfluidic chambers capable of generating fluidic microvortices to rotate suspended cells. We then compared several microfluidic chip designs experimentally in terms of: (1) their ability to rotate biological cells in a stable and precise manner; and (2) their suitability, from a geometric standpoint, for microscopic cell imaging. We selected a design that incorporates a trapezoidal side chamber connected to a main flow channel because it provided well-controlled circulation and met imaging requirements. Micro particle-image velocimetry (micro-PIV) was used to provide a detailed characterization of flows in the new design. Simulated and experimental results demonstrate that a trapezoidal side chamber represents a viable option for accomplishing controlled single cell rotation. Further, agreement between experimental and simulated results confirms that numerical simulation is an effective method for chamber design.
Cell biology and microbiology applications that require the ability to quantify genotype, gene expression, and phenotype at the single cell level are becoming increasingly important. Single-cell studies can quantify the heterogeneity among cells within populations, including isogenic cell populations. Historically, biological research relied primarily on bulk cell studies, which quantify averaged cellular parameters and responses. However, ensemble averaged measurements fail to explain how a subgroup of cells respond or survive in the face of a sudden perturbation such as a rapid environmental change or the administration of a drug. Quantification of intercellular differences through single cell analyses can offer insight into the benefits or consequences of cell population heterogeneity [
Cell morphology provides useful information for diagnosing diseases. For example, irregular nuclear size, shape, and internal chromatin distribution can suggest cancer initiation and portend progression [
Multiple-perspective microscopy accesses 3D cellular structure with fewer drawbacks and also enables volumetric image reconstruction. For example, 3D single cell computed tomography (Cell-CT, VisionGate, Phoenix, Arizona) acquires a series of pseudoprojections taken by optical microscopy from various angles, and applies the filtered backprojection method to reconstruct a 3D image of the cell. The method is similar to x-ray computed tomography (CT) [
Schematic illustrating cell rotation in a trapezoidal side chamber. Illumination is provided by a broadband light source and condenser. A cell is stabilized at the desired position by optical trapping in a trapezoidal side chamber. Projection images may be acquired at different angles as the cell is rotated by hydrodynamic forces.
On the micro-scale level, mechanical micromanipulation provides precise user control with multiple degrees of freedom for orienting single cells [
We use Computational Fluid Dynamics (CFD) to facilitate microvortex chamber geometry design and flow field simulation. CFD was previously used to evaluate and compare diamond- and double diamond-shaped side chamber designs [
Researchers have also performed experimental characterization on microvortex flow fields. Shelby
We applied micro particle-image velocimetry (µ-PIV) to investigate recirculating flows in microscale side chambers. Adrian introduced the first µ-PIV systems to demonstrate slow flow measurement with micrometer resolution [
To understand the effect of side chamber geometry, a first set of simulations was performed to characterize rectangle-shaped, isosceles trapezoid-shaped, and diamond-shaped side chambers, each attached to a long, straight channel. All CFD simulations were performed in 2D. The simulated main channels were 700 µm long and 50 µm high. The opening between each channel and chamber was 40 µm. The trapezoid- and rectangle-shaped side chambers were 60 µm high, and the diamond-shaped side chamber was 130 µm high. The longer parallel side of the trapezoid-shaped side chamber was 140 µm. The lower half of the diamond-shaped side chamber was identical to the trapezoid-shaped chamber.
Channel and side chamber geometries were meshed with Gambit (ANSYS, Canonsburg, PA). 5,125 structured quadrilateral elements were applied to mesh the main flow channel. Since the microvortex forms within the side chamber, node density was set to 1 µm/node in each side chamber to ensure detailed grid resolution there. Structured quadrilateral meshes were applied for the rectangle-shaped side chamber, which resulted in 2,400 quadrilateral elements. A combination of unstructured quadrilateral and triangular meshing was applied for the diamond-shaped and trapezoid-shaped side chambers. The diamond-shaped chamber comprised 10,151 elements, while the trapezoid-shaped chamber comprised 5,360 elements
Fluent 6.2 (ANSYS, Canonsburg, PA), was used to simulate 2D velocity profiles in the chambers. The SIMPLE algorithm was applied to couple pressure and velocity, a no-slip boundary condition was applied at the walls, and water was used as the working fluid. A uniform 20 µm/s inflow velocity was applied at the inlet and an outflow boundary condition was applied at the outlet for all three cases in the first set of simulations. It is noteworthy that uniform inflow velocities ranging from 0.01 µm/s to 1 m/s, all of which correspond to flows within the laminar regime, were also examined for a subset of the side chamber designs. Over that range, there were no apparent changes to the shapes of the side chamber flow structures.
We compared simulation results for the different chamber geometries and, based on performance and optical accessibility, we selected the trapezoid-shaped side chamber as the most advantageous design. For the second set of simulations, the trapezoid-shaped design was further optimized based on our design requirements, including ease of prototyping. More specifically, the chamber opening was made as large as possible (so as to facilitate cell entry and nourishment, and also to avoid sharp corners that are problematic for microfabrication), without elevating the chamber opening to main channel height ratio above 0.8. That value was identified as a maximum conservative threshold, based on previous simulations, which must not be exceeded in order for the microvortex to form. Further, the chamber height was made as large as possible (again to avoid sharp corners), without surpassing the main channel height, beyond which point the microvortex position within the chamber becomes problematic for imaging (again based on previous simulations). In the final design, the main channel was made 800 µm long and 70 µm high, and the trapezoidal side chamber was made 70 µm high, with a 140 µm longer side and a 56 µm shorter side. The main flow channel was meshed with 8,890 structured quadrilateral elements, and the chamber was meshed with 7,436 unstructured quadrilateral and triangular elements. A uniform 23.8 cm/s inflow velocity was applied at the inlet for this second set of simulations and results were then compared with experimental data. It is noteworthy that geometric optimization of the chamber in this application was driven by fairly simple design constraints, so advanced geometric optimization methods were not required; however, in cases characterized by more complex and demanding constraints, successful design may benefit from if not require a highly capable geometric optimization strategy [
Based on the optimized geometry, a microfluidic device was fabricated in polydimethylsiloxane (PDMS) with standard soft lithography methods using a 60-µm-thick KMPR mold. The fabrication process is summarized as follows: First, PDMS monomer and cross-linker were mixed at a ratio of 10:1. Second, the mixture was degassed in a vacuum chamber for at least 30 min until all air bubbles disappeared. PDMS was poured into the KMPR mold and cured overnight. After curing, inlet and outlet holes were created by punching with an 18-gage stainless steel tube. PDMS chips and cover glasses were treated by a plasma cleaner (PDC-32G, Harrick Plasma, Ithaca, NY) for 40 s. After attaching the treated surfaces together, the PDMS layer was permanently bonded to the microscope cover glass.
A volumetric flow rate of 1 µL/s was supplied by a gear pump (milliGAT, GlobalFIA, Fox Island, WA), which provided an approximately uniform flow profile at the inlet of the microfluidic device with a mean flow rate of 23.8 cm/s. Fluorescent particles of 1-µm diameter (PSL, TSI Inc., St. Paul, MN) were diluted 10x before seeding. A 2D µ-PIV system (Micro-PIV, TSI Inc., St. Paul, MN) was used to obtain a series of consecutive image pairs within the flow field. A schematic of the experimental setup is shown in
A filter cube containing a dichroic mirror filter and a barrier filter was integrated with the inverted microscope. The light path in the system is shown in
Using a 20×, 0.45 NA objective lens, the entire trapezoidal side chamber could be seen within the field of view. The focal plane was adjusted to the central plane of the microfluidic device because the particle density was highest in this plane. In addition, PIV particles can slowly recirculate near the flow channel and microchamber walls, causing the particles to lag behind the flow [
Micro particle-image velocimetry experimental setup. Inset shows trapezoidal side chamber.
A set of 400 image pairs was captured for each experimental run. The time separation between images in a pair was set to 70 µs to ensure that particle displacements within the trapezoidal chamber were less than 10 pixels. Raw images were processed after capture using DaVis software (LaVision, Ypsilanti, MI). Background noise was first removed by subtracting the mean intensity of 400 image pairs from each image. A mask with the shape of the trapezoidal side chamber was applied to exclude everything outside of the flow region. Velocity vectors were calculated using a multi-pass, recursive cross-correlation algorithm with 50 percent grid overlap. In this study, the initial interrogation window was set to 64 × 64 pixels, and the final window was set to 32 × 32 pixels. The size of the initial interrogation window was chosen as 64 × 64 pixels to accurately capture the range of velocities within the chamber. However, this choice of interrogation window size resulted in an experimental design tradeoff favoring measurement accuracy within the chamber at the expense of measurement accuracy in the main flow channel, where higher velocities were present. A Gaussian weighting function was also applied to the correlation surfaces calculated for each interrogation window in order to deemphasize outliers.
Three different side chamber geometries were simulated and performance was compared in terms of vorticity and vortex position. Vorticity is the curl of the velocity field
Columns show CFD simulations of the diamond-shaped (
In order to create a microscopically accessible hydrodynamic microvortex for multiple-perspective imaging, a flat side plane parallel to the main channel is desirable. The diamond-shaped side chamber with a corner at the imaging surface does not provide imaging accessibility. Rectangular and trapezoidal side chambers on the other hand both provide excellent optical access. However, for the same chamber opening size, the trapezoid-shaped chamber offers superior accessibility for imaging. The vortex center of the trapezoidal side chamber is further away from the main channel than the rectangular one. The position of the vortex center is an important parameter since further displacement from the main flow channel, toward the imaging surface, can improve rotational stability and decrease the likelihood of a cell being dragged out of the microchamber and into the high-velocity main channel. The trapezoidal chamber displaced the vortex center 6.3% closer to the imaging surface than the rectangular chamber. Total circulation
The trapezoidal chamber achieves 61 percent more circulation than the rectangular chamber. One explanation may be that the obtuse angles (sharper corners) at the channel neck of the trapezoidal chamber separate more flow from the main flow channel than the right angles of the rectangle-shaped side chamber. Furthermore, the vorticity contours in
A comparison of the velocity and vorticity magnitude contours from µ-PIV experiments and simulations using the trapezoidal chamber is shown in
Velocity and vorticity magnitude contours from µ-PIV (
The velocity magnitude surface plots for both µ-PIV experimental data (a) and simulated data (b) are shown in
Comparison between µ-PIV (
In order to quantitatively compare the PIV results with CFD, a statistical analysis was performed. It is noteworthy that velocity magnitude in the trapezoid chamber was not normally distributed, so we applied a nonparametric data analysis that does not require data normality and provides the additional advantage of being robust to outliers. Specifically, the Kolmogorov-Smirnov two-sample test was used to test the null hypothesis that PIV and CFD velocity magnitudes were identical with respect to location and dispersion. First, we smoothed the CFD resolution to the µ-PIV level (2.88 µm/node) with Kriging interpolation [
To demonstrate the successful design and fabrication of the trapezoidal side chamber, pre-cancerous human esophageal epithelial cells cells were introduced into the chamber and rotated at controlled rates. A time series of images showing controlled rotation of a dysplastic esophageal epithelial cell (CP-C cell line) is provided in
Time series images demonstrating the controlled rotation of a CP-C cell through 180° over 0.51 s.
The trapezoidal side chamber provides excellent optical access. Using an appropriate cell culture medium as the working fluid, the device can also provide a suitable environment for living cells. The chamber design is compatible with rotating single cells at a controllable rate, providing a viable image acquisition process for multiple-perspective imaging systems. Specifically, cells have been rotated within the chamber at rates ranging from 70 to 120 rpm in continued work, with a validated rotation rate accuracy of approximately +/− three percent. Future work will include advanced CFD model development, including modeling of both the trapped object and the optical trapping beam. The µ-PIV experiment can also be improved by using smaller tracing particles and a more advanced, perhaps elastic, particle tracking algorithm. Lastly, the realism and/or relevance of the experimental flow profile could be further enhanced by introducing a trapped microparticle or cell into the µ-PIV-monitored device.
This article presented a process, guided by numerical simulation, for designing microvortex side chambers. We selected the trapezoidal side chamber design for our application, after comparing performance among alternatives, because it provided the best optical access and microvortex center position. Our µ-PIV experimental approach was used to achieve a specifically purposed flow profile within a trapezoidal side chamber, which has not been proposed in other studies. The design process was further supported by simulation results, which agreed with µ-PIV results at the 95 percent confidence level, and the proposed design has now been used successfully to accomplish controlled rotation of real cells. Future work will include broader experimental cell studies and cell imaging, as well as more advanced computational studies that focus on other important fluid dynamic characteristics of the proposed chamber design, such as the shear stress profiles experienced by rotating cells under variable main channel flow conditions.
This research was supported in part by a grant from the W.M. Keck Foundation (D.R. Meldrum, PI).


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text:
Channeling the frenzy. Ever since a Wal-Mart employee on Long Island was trampled to death on Black Friday in 2008, retailers have been alert to dangers of stampeding shoppers. "Stores thought that creating this frenzy was good for business," Marsha Cohen, chief retail analyst for the NPD group tells The Boston Globe. "As it turns out, this puts retailers in a very precarious legal position and it creates a very inefficient system for consumers to take advantage of sales."
This year, the Globe reports, Target is creating a buffer zone of at least 10 feet between entrances and the start of the lines. Groups of 30 people will be allowed into the buffer zone every 20 or 30 seconds to keep mobs from rushing en masse into the store.
Did you check online? Consumer Reports notes that many retailers offer the electronic goods at the lowest price of the year. But even then, CR says, the best bargains may not be at the brick-and-mortar stores. Don't forget to check Amazon.com and other websites, which often can do better. (Amazon is even starting its Black Friday deals early.)
Brick-and-mortars, though, are also using the Web to build excitement and lure buyers into the store. Best Buy already has announced some Black Friday deals on the Internet and promises more on its Twitter and Facebook pages this week. Already on tap: a 24-inch high-definition Dynex TV for $80, a Samsung laptop with a 320GB hard drive for $300, and a Toshiba Blu-ray disc player for $40.
The tech lure. Consumer Reports' James Willcox offers this advice when shopping for electronic goods:
Often retailers — and sometimes, manufacturers — will combine two or more items into a specially priced package they say will save you money. But you should check the individual prices of these items at both the store and its competitors to see if you're really getting a great deal. Our research during the past week uncovered some real savings, but also bundles that barely saved any money at all.
Hottest deals. Fatwallet.com, one of the top sites for deals, offers a user-friendly Deal Finder, to check out sales either by category or by stores. Here are some of its hottlest Black Friday offerings so far this year:
1. Norton Internet Security 2012 (1-PC) [Boxed CD] - Staples - Free
2. Toshiba Satelite C655D-S5332 15.6" Laptop - Radio Shack - $299.99
3. Xbox 360 4GB Console w/Kinect - Wal-Mart - $199.96
4 . Acer 23" Widescreen LCD Monitor - Staples - $99.99
5. BlackBerry PlayBook 7" Tablet - 16GB, WiFi - Staples - $199.99
Fast and furious. It seems almost impossible to keep track of the waves of ads that are coming as BF looms. One tip: Bradsdeal.com puts the big ones all on one page for a quick peek. Here's one you'll find from Ace Hardware: A Radio Flyer red wagon for $49.99.
Doug is an unrepentant news junkie who loves breaking news and has been known to watch C-SPAN even on vacation. He has covered a wide range of domestic and international news stories, from prison riots in Oklahoma to the Moscow coup against Mikhail Gorbachev. Doug previously served as foreign editor at USA TODAY. More about Doug
Michael Winter has been a daily contributor to On Deadline since its debut in January 2006. His journalism career began in the prehistoric Ink Era, and he was an early adapter at the dawn of the Digital Age. His varied experience includes editing at the San Jose Mercury News and The Philadelphia Inquirer.
Also: Follow On Deadline on Twitter


text:
Howard Jones is a duck shooter and an irrigator who grows wine grapes near the Murray River town of Dareton in southern NSW.
But you won't catch this weather-beaten farmer, hunter and fisherman burning copies of the Murray-Darling Basin Authority's proposals to shift big volumes of water from agriculture to the environment.
While the angst and anger expressed at community meetings since the authority released its proposals two weeks ago October 8has captured the headlines, there are farmers like Jones who believe the river system they rely on is in desperate need of revival.
Indeed, Jones has urged Environment and Water Minister
"We need the [basin] plan," he says. "Sure, there's flaws in it as there are in any draft document. We need to work through those issues and the government needs to get on with buying water.
"If we don't have a healthy river system, we don't have a life."
Jones was part of a delegation that was convened by conservation groups and spoke to Burke on Wednesday night, a day after he heard from farm lobby groups that the proposed reforms would cost thousands of jobs.
While the irrigation groups urged Burke to invest more in water efficiency infrastructure rather than buybacks to maintain the productive capacity of regions, Jones put the opposite case.
"The bulk of the money should be spent on buybacks," he says. "Efficiency measures are usually expensive and take a lot of time. We need more water back in the system and buybacks are the way to go."
Jones has been an irrigator for 30 years and may seem an unlikely conservationist. He became involved in efforts to restore wetlands in the 1990s after noting the degradation of the river system while hunting and fishing.
"I was going around seeing places where you'd normally wander along and ping a duck or catch a fish and all of a sudden it was clear there was something going on," he says. "The trees were dying or the reeds looked pretty ordinary, the water was starting to smell or was not there at all."
Now he chairs a group called Murray-Darling Wetlands Limited, which is dedicated to restoring environmental sites and is prepared to argue for reform, even if he knows many other irrigators see it differently.
Jones intends to hang on to his licence for 400 megalitres of high- security water from Western Murray Irrigation and continue growing wine grapes.
But he says that if the government offered the right price – significantly above what it has offered so far – he believes many irrigators in the region would take the money and retire.
"If they hit the right price target, quite a few people I know would sell. Most of us are on the wrong side of 60. There is another life apparently," he says.
He acknowledges mass buybacks come at a cost to the community and says there would need to be some targeted financial support, as well as buybacks.
In its 10-year Murray-Darling plan, the government promised $3.1 billion for irrigation licence buybacks from 2008 and $5.8 billion for water infrastructure projects.
During the election campaign it promised it would secure any extra water that was required for the environment through purchases from willing sellers.
So far, the focus has been on buybacks but, faced with the backlash of recent weeks, the government has begun talking up the water savings that could be achieved through infrastructure measures, particularly to improve the management of environmental water.
On Wednesday, National Farmers' Federation CEO
If that occurred, he said any attempt at water reform would fail and could end up pitting farmer against farmer.
The Australian Financial Review


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text:
What is CHINATRACE
It is a joint venture founded by TraceTracker, international leader in global information exchange for the food industry, and Shandong Institute of Standardization, national leader in developing and implementing traceability solutions.
CHINATRACE is an extension of the TraceTracker GTNet®, which is a web platform with a suite of solutions that enhance the value of products and brands while stimulating consumer dialogue. Shandong Institute of Standardization provides the foundation for this service through reputable solutions and expertise in implementing traceability systems.
Mission and Vision
Our mission is to assist the food industry and authorities in preventing, containing and resolving food quality and safety issues. At the same time, we contribute to continued growth and long term perspective for Chinese food exports and domestic consumption.


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Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
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Philip Levine
Philip Levine (January 10, 1928 / Detroit, Michigan)
» Click to list general statistics » OR, select a date :
Ode For Mrs. William Settle
In Lake Forest, a suburb of Chicago,
a woman sits at her desk to write
me a letter. She holds a photograph
of me up to the light, one taken
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text: Walking the streets of NYC, you come across a fairly large amount of strollers. Bugaboo, Bugaboo, Bugaboo, Bugaboo, Maclaren - no, wait, that's just an older Bugaboo. As you can imagine, the stylishly designed Bugaboo brand is quite popular. I'm sure you've seen this in other urban areas. The one thing that was pretty much the same was the canopy. Well, you can now buy new canopies called 'Bonne Bonnets' (from a company of the same name) in lots of cool, sassy patterns made to fit any Bugaboo stroller. I like the 'Neon Spring' one, but there's a nice selection to choose from.


text:
Big Easy Scarf by Lee Samsami Stein
Welcome! You are browsing as a guestWould you like to see 35 projects made from this pattern and much more? create a free account What am I missing?
© Lee Samsami Stein
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© Lee Samsami Stein
Big Easy Scarf
This pattern is available as a free Ravelry download
This scarf, made from leftover yarn from a bigger project, utilizes the faggotting pattern called Turkish Stitch, and I love it because it's reversible, and painfully simple to knit. (from Barbara Walker's Treasury of Knitting, Vol. 1). It's the perfect tv-watching project, and makes a great last-minute gift as it won't take more than a day to finish!


text:
You Only You - Poem by SWETA LEENA PANDA
You are my life
You are my wealth
You are my everything
You are the reason for which I wanna win
Yes, it's you
Only you,
Yes, I am telling to you
Can you hear me?
What I am saying
I need you here
I need you in my every life
I need you love
You are my hero & my simulate too
I can't win, I can't win in the betel field without you
I can't win this game without you
Without you I feel I am lost, I am vain
I can't think about a life a day without you
Can you give me a promise to stay with me for forever?
Yes, I am telling to you
Yes, I only need you
can you hear me what I am saying
I Won't run, I won't Fly,
I can't rest, I can't fight
I am nothing without you
only without you
Yes, I am telling to you
Can you hear me what I am saying
I won't soar, I won't climb
I'm paralyzed, I'm so blind
I lost my mind without you,
only without you
You only you
Ohh can you hear me
What I am saying
I am seeing only to you
I need only you
You, you only you
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Edgar Allan Poe


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text:
The scene was set and the New England Patriots were ready to take a victory from the New York Jets in the Meadowlands on Sunday … and as we have seen time and time again in the past two seasons, they let the game go.
When looking back on last season, of the six games the New England Patriots lost, four of them were lost after leading or being tied going into the second half.
The last three regular season games for the Patriots are a good illustration of what I mean. In the last game of the 2009 campaign, the Patriots lost 34-27 to the Houston Texans after leading 20-13 after three quarters. In the Cincinnati Bengals opener, the Patriots had a 24-3 halftime lead and then were outscored (21-14) and outgained (272-128) in the second half. The scoring spree made the game a lot closer than what it should have been. Then there was Sunday versus the Jets where three crucial turnovers in the second half gave the game away to New England's bitter AFC East rivals.
Why have the New England Patriots -- who, until recently have always been able to close out games in the past -- become a team that squanders their lead late in games? Is it because of the team being so young and no veteran leadership presence? Is it because of lack of conditioning causing the players to wear out before final gun goes off in the game?
These are all questions that need to be addressed soon if the Patriots are going to make a run at the Lombardi this year.
When coach Bill Belichick was asked about the loss on Monday he answered, in typical Belichick fashion "We just didn't do well enough," adding "If there were some switch, I would flip it."
Even quarterback Tom Brady was befuddled when asked about the letdowns in the second half. "I think when it comes down to these second-half games and our level of toughness, we've got to go out there and play tougher," Brady said. "I have to do a better job of leading this team and certainly execute better when it's crunch time."
That was a good answer by Brady but where is this toughness and leadership going to come from? Is it going to be safety Brandon Meriweather, now in his fourth year, or will it comes from third year linebacker Jerod Mayo?
Leadership can come in many different forms on this team but to put it simply, it has to come from somewhere within. With the loss of RB Kevin Faulk, of one of the team's captains, due to a torn ACL, there is one less veteran leader to look to.
Before 2009 when the team was pushed around, players like Rodney Harrison and Tedy Bruschi were there to help to make adjustments and lead the younger players through the tough times.
Obviously if the head coach or the quarterback have no idea what causes the meltdowns in the second halves, then we have to make assumptions as fans of the game. The top assumption would be that the team lacks mental toughness and on-the-field leaders. The team needs to focus onplaying 60 minutes of football in order to finish off the games that they should be winning.
The other portion of this that cannot be brushed aside is the coaching in these losses. When teams go into halftime, the crucial piece of success is the preparation and game planning in the locker room at halftime. In both games to start this season, the second half adjustments that Marvin Lewis and Rex Ryan made were far more successful than the ones Bill Belichick and the coaching staff in New England came up with. So, not only does this team need leadership on the field and in the locker room, they need a game plan that will put them in a place to win.
So it is simple right? Go into halftime, make the adjustments and come out and rely on your leaders to execute the strategy. Easy enough, correct?
I guess that is why I sit at home and write instead of playing on the field … sometimes the phrase "easier said than done" comes to mind
Hopefully the Patriots can get in the right mindset with the struggling Buffalo Bills coming to Foxboro this Sunday.
Shane Leketa is a long time Patriots fan who bleeds red white and blue. Leketa runs the site @MyPatsSpace


text:
Rent Musical Ritual in Mexico City 1st edition today, or search our site for other textbooks by Mark Pedelty. Every textbook comes with a 21-day "Any Reason" guarantee. Published by University of Texas Press.
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text:
Lake Erie, Colorado's AHL affiliate, had the unenviable task of playing three games in three nights. After taking down Peoria and Milwaukee Thursday and Friday night, respectively, the Monsters (7-4-0-0) fell to Rockford in penalty-filled fest.
In the game against the Rivermen, Lake Erie battled back down two goals in the third period to win in a shootout, 4-3. Captain Bryan Lerg opened the scoring in the first for the Monsters with a power play goal from Tyson Barrie and Thomas Pock.
After two goals by Adam Cracknell and one by Andrew Murray made it 3-1 for Peoria, Cameron Gaunce added his first goal of the year on a wraparound bank shot off goalie Jake Allen's skate to cut the deficit to one. Less than a minute later, Andrew Agozzino tied it at three on wrist shot stick-side, with Barrie drawing his second assist of the game.
After overtime settled nothing, Lake Erie got goals by Lerg, Mike Sgarbossa and Mark Olver and goalie Sami Aittokallio only giving up one goal on four shots in the shootout. He also stopped 25 of 28 shots in regulation and overtime for the win.
Calvin Pickard drew the assignment in game two against the Admirals Friday. He stole the show with a 42-save performance and a 2-1 win.
Milwaukee drew first blood on the power play, with Michael Latta putting home the rebound at 12:24 of the first period. Lerg tied it up with only 17 seconds remaining in the first on the man advantage. Luke Walker and Sgarbossa earned the assists.
After Monster forward Geoff Walker took a holding the stick penalty, Luke Walker pounced on a rebound from Paul Carey to score the short-handed goal in the second. That was all Pickard needed, stopping all 31 shots in the second and third periods.
Lake Erie jumped ahead Saturday 3-1 but penalties were their demise, as the IceHogs came back and won 6-3 in a game that saw 120 combined penalty minutes and four ejections.
Bill Thomas, Agozzino and Barrie scored the three goals for the Monsters, who do not play again until Friday when they head to the Toyota Center to play the Houston Aeros. Patrick Bordeleau ended the game with 26 minutes in penalties. Pickard made 27 saves in the loss.
Rockford had six different goal scorers. Adam Clendening, Shawn Lalonde, and Brandon Pirri scored goals a man up while Brandon Bollig, Kyle Beach and Jeremy Morin scored at even strength. Carter Hutton earned the win for the IceHogsTags: Calvin Pickard, Colorado, Colorado Avalanche, Hockey, Lake Erie, NHL, Tyson Barrie


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2015 Performances and Workshops
with Karen Montanaro
January 1 • Performance Description. www.performancelink.org
Exciting News!
Karen to present her "Talk"
at a prestigious conference!
New Promo Pics Click Here!
Photocredit: George C. Anderson
A Visit to Sanford (News Article)
Photocredit: Argy Nestor
Bringing Magic (News Article)
Photocredit: Times Record
School Assemblies Click Here!
Photocredit: The Sun Journal
A Dance To Touch The Soul
Concerts CLICK HERE!
Workshops CLICK HERE!
Presenter Resources CLICK HERE!
Karen with Marcel Marceau
Read More in "Backstage"


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In the mood for fall comfort food but don't want the extra fat? Here is a great and super easy recipe for you!
Low Fat Pumpkin Brownies
1 box low fat brownie mix
1 15 oz. can of pumpkin
Mix can of pumpkin in with the mix and mix well.
No need to add any other ingredients.
Bake according to directions on box.
Makes about 16 servings.
How to Make a Mermaid Crown
1 hour ago


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(Video) Ask the Expert Webcast: Anyconnect Configuration and Troubleshooting
- Gold, 750 points or more
is a customer support engineer and CCIE at the Technical Assistance Center at Cisco. In this webcast, Vikas provides information on configuration basics of AnyConnect SSL VPN Client on ASA through ASDM. He also provides information on concepts of profiles, tunnel groups, group policies, and troubleshooting of common problems associated with AnyConnect SSL VPN client. He has answered many questions asked from the audience during the live event.


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Remote Methode Call is a project to enable calls to methods in remote C++ classes. The transport is implemented as a library, and is free from dealing with the internal RMC stuff, so any C/C++ programmer is able to write a transport for his own purpose. RMC(2) currently has a sockets transport library as reference implementation. It is developed on Linux, and supports Linux on iX86 and PPC. RMC should also work on BeOS. Testers and developers are welcome to the project.
|Internet Clustering/Distributed Networks Networking
|Operating Systems
|BeOS Windows POSIX Linux
A Fluent OpenStack client API for Java.
A TWiki appliance that is easy to use and lightweight.


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Our Friends
Below is a list of links to follow for more area information:
dreamsvilleusa.com- Welcome to Dreamsville, USA. Home of Dennison and Uhrichsville, a great way to find out what we have to offer.
www.traxdiner.com- The Trax Diner is located within the Dennison Railroad Depot Museum and offers a variety of lunch and dinner specials.
ohiosfirstvillage.com- Historic Schoenbrunn Village, the first settlement in Ohio started by the Moravians. Schoenbrunn is operated by the Dennison Railroad Depot Museum with an agreement through Ohio Historical Society.
www.claymuseum.com- The Uhrichsville Clay Museum is located in Uhrichsville, "The Clay Capital of the World". Visit today to view the clay industry at its finest.
http://schoenbrunnvillagefair.org/- Schoenbrunn Village Colonial Trade Fair, Held October 17th & 18th 2009www.neohiotravel.com – Come visit the local Tuscarawas County Convention and Visitors Bureau to see all the attractions in the area.
cityofuhrichsville.org/waterpark – Uhrichsville Aquatic Park now open from 11:00am – 8:00pm Sunday – Saturday. Complete with slides, waterfalls, fountains, and much more!
www.ohiorailtourism.org – The Dennison Railroad Museum is a proud member of the Ohio Rail Tourism Association(ORTA).
www.smartrving.net – Special military Active-Retired Travel Club
www.5ad.org – 5th Armored Division
www.trainorama.com – Train-O-Rama has Ohio's largest operating multi-gauge model railroad display open to the public
http://www.bradfordrrmuseum.com/ – Bradford Ohio Railroad Museum
http://www.100thjager.com – WWII historical re-enactment unit formed to portray the 100th Jager unit as it would have appeared in 1943 –1945. A great part of the annual American Soldiers Homecoming Festival held in August.
Links for Pictures


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Longview's Zaycoven Henderson Commits to Texas + Will Enroll in January
LONGVIEW — The Texas Longhorns have another East Texan headed to the 40 Acres.
Longview senior defensive tackle Zaycoven Henderson announced his commitment to Texas during a Monday afternoon ceremony at Longview High School. Henderson plans to graduate this month and enroll at Texas in January.
The 6-foot-1, 300-pound standout had about 20 offers to choose from, but picked the Longhorns over Texas Tech and Baylor, two schools that joined Texas in his final three.
Henderson decommitted from TCU in early September. Since then, Texas had been considered the favorite in his recruitment. Henderson had Texas and Texas Tech shirts in front of him for his announcement, but slipped on the burnt orange to make it official. Family members and friends decked out in Texas gear also tipped off spectators as to which school the standout defensive lineman was about to choose.
"It's the University of Texas. You can't go wrong. They send D-linemen out every year (to the NFL). It's my state's school. Why not?," Henderson said of his decision to go to Austin.
He also said that the perceived uncertainty regarding longtime head coach Mack Brown's future had no impact.
"I'm not playing for the coaches. I'm playing for me, and I'm playing for my family and my support," Henderson said. "The coaching staff, it's still gonna be Texas. They're gonna get the best coach. It's one of the most prestigious programs in the country."
Henderson added that classmate Dorian Leonard, a 6-foot-4 receiver who committed to Texas in mid-June, being a Longhorn pledge influenced his decision.
"That's my boy," said Henderson, who is determined to play as a freshman. "I never had the offer. He had it. When I got it, that's when I decided to go.
"They're great in education. My family, that's all we talk about is education. Make sure you go in with a play. I'm going there with a plan and I'm gonna major in business."
Henderson is the fifth East Texan and fourth in the 2014 class to commit to Texas, joining Leonard, Jasper senior center Terrell Cuney, Newton senior athlete Kevin Shorter, and Lufkin junior athlete KeKe Coutee.
Henderson overcame a foot fracture that cost him the first five games of the 2013 season. In only eight games, he recorded 36 tackles, 13 for loss, two sacks, one forced fumble, one fumble recovery, and one defensive touchdown. That performance came after his breakout junior season a year ago, when he recorded 48 tackles, 10 for loss, and two sacks.
You knew that when we got the good-on-good, that Zay was ready to go. He would get the party started, so to speak. — Longview coach John King on Henderson's leadership in practice
Longview head coach John King said Henderson did not waste any time getting back to business once he was cleared to play following the foot fracture.
"He was ready to get back on the football field. He didn't milk it. As soon as he could get back out there, the next day he was on the field playing fighting through pain, and continued to do so throughout the rest of the season," King said. "The biggest thing is Zay will mix it up with anybody. The nastier it gets the better he's gonna play. He's just kind of a throwback, old-school defensive lineman.
"The thing that stands out the most about him is his first step. Extremely violent, and strong with his hands. When he throws blockers off of him he can chase the football. We played him inside as a nose and a 3-tech, and then we moved him to the edge as a 5-technique and he was effective both places."
King, who has coached his share of high-major college signees, said Henderson has everything physically and mentally to be an impact player in a major conference.
"Zay's gonna fit in anywhere. He's a very likable kid and very intelligent. He's an extremely smart football player. He just understands the game of football, scheme-wise and leverage-wise," King said. "He understands the intensity you've gotta play the game with. His work ethic, what he's shown us here and what he's been able to do here, I think it's gonna carry him throughout his college career and hopefully one day he may play on Sunday."
East Texans Committed to Texas
2014 Class
- Terrell Cuney, C, Jasper, 6-3, 260
- Zaycoven Henderson, DT, Longview, 6-1, 300
- Dorian Leonard, WR, Longview, 6-4, 200
- Kevin Shorter, RB/ATH, Newton, 6-0, 190
2015 Class
- KeKe Coutee, ATH, Lufkin, 5-10, 160
2014 East Texas Football Players With Division I FBS Offers
- Terry Ausborne, S, John Tyler, 5-10, 175 — offer from SMU (1)
- Blake Bogenschutz, QB, Carthage, 6-1, 175 — committed to UTSA (1)
- Jacob Bragg, OL, Nacogdoches, 6-4, 310 — committed to Kansas; additional offers from Louisiana Tech, Oregon State, ULM (4)
- K.D. Cannon, WR, Mount Pleasant, 6-0, 160 — committed to Baylor; additional offers from Arizona, Clemson, Florida State, Houston, Miami, Nebraska, Notre Dame, Oklahoma, Oklahoma State, Ole Miss, Purdue, SMU, TCU, Texas, Texas A&M, Texas Tech, Utah, Vanderbilt, Virginia, West Virginia (21)
- Caleb Chumley, QB/ATH, Pine Tree, 6-5, 220 — offers from Houston, Kansas, Louisiana Tech, Memphis, Missouri, New Mexico State, North Texas, Oregon State, Texas State (9)
- Joseph Clark, RB/S, Chapel Hill, 5-9, 175 — committed to Texas Tech; additional offers from Air Force, Navy, Texas State (4)
- Kade Clayton, ATH, Tyler Grace, 6-0, 180 — offers from Navy, SMU, Texas State (3)
- Terrell Cuney, C, Jasper, 6-3, 260 — committed to Texas; additional offers from TCU, Texas Tech (3)
- Devondrick Dixon, ATH, Gilmer, 5-9, 180 — committed to Texas State (1)
- Adrian Goodacre, OL, Carthage, 6-4, 310 — committed to ULL; additional offer from Houston (2)
- Terian "Tee" Goree, WR, Carthage, 6-2, 170 — committed to North Texas; additional offers from Colorado, Colorado State, Louisiana Tech, Memphis, San Diego State, ULL, ULM, UTSA (9)
- Davion Hall, ATH, Liberty-Eylau, 6-2, 191 — committed to Baylor; additional offers from Alabama, Arkansas, LSU, SMU, Stanford, Texas, Texas A&M, Texas Tech (9)
- Zaycoven Henderson, DT, Longview, 6-1, 293 — committed to Texas; additional offers from Baylor, Florida, Iowa State, Kansas, Kansas State, Missouri, Nebraska, Oklahoma, Oklahoma State, Ole Miss, Oregon, SMU, South Carolina, TCU, Texas Tech, Washington, Washington State (18)
- Travin Howard, S, Longview, 6-0, 178 — offer from UTSA (1)
- Willy Ivery, ATH, Sulphur Springs, 5-8, 180 — committed to North Texas; additional offers from Colorado State, New Mexico, Texas State, Toledo (5)
- Jamal Jeffery, WR, Lufkin, 5-9, 165 — committed to Texas A&M (1)
- Chris Jefferson, ATH, Athens, 6-2, 200 — committed to SMU (1)
- Jawuan Johnson, DB, New Boston, 6-0, 195 — committed to Northern Illinois (1)
- Dorian Leonard, WR, Longview, 6-4, 200 — committed to Texas; offers from Florida State, Oklahoma, Oregon State, San Diego State, Texas Tech (6)
- Reggie Long, LB, Whitehouse, 6-0, 218 — offers from Army, New Mexico State (2)
- Ben Luckett, RB, Palestine, 6-0, 195 — committed to ULM (1)
- Patrick Mahomes, QB, Whitehouse, 6-3, 203 — committed to Texas Tech; additional offers from Houston, North Texas, Rice (4)
- Stephan Maxey, DB, Longview, 5-11, 185 — offer from UTSA (1)
- Keith Minor, DL, Chapel Hill, 6-3, 237 — offer from Cincinnati (1)
- Jarrell Owens, LB/DE, Palestine, 6-3, 230 — committed to TCU; additional offers from Texas State, Texas Tech, ULM (4)
- Greg Roberts, DE, Nacogdoches, 6-5, 200 — committed to Baylor (1)
- Anthony Rhone, LB, Texas High, 6-0, 210 — committed to SMU (1)
- O'Keeron Rutherford, WR, Carthage, 6-5, 181 — committed to North Texas; additional offer from ULM (2)
- Anthony Sanders, ATH, Athens, 5-11, 185 — offer from ULM (1)
- Kevin Shorter, RB/S, Newton, 6-0, 190 — committed to Texas; additional offers from Arkansas, Army, Baylor, Clemson, Florida State, Georgia Tech, Houston, Kansas, LSU, Maryland, Miami, Mississippi State, Nebraska, Ohio State, Oklahoma, San Diego State, Tennessee, Texas A&M, Texas Tech, UCLA, ULM, Utah (22)
- Brian Thlang, OL, Palestine, 6-2, 290 — committed ULM (1)
- Wesley Thompson, ATH, Chapel Hill, 6-1, 185 — committed to ULM (1)
- Josh Walker, RB/LB, Gilmer, 6-1, 220 — committed to Texas A&M; additional offers from Oklahoma, Texas, Texas Tech (4)
- D.J. Williams, DT, Lufkin, 6-4, 293 — offers from Houston, Miami, Missouri, North Texas, Oklahoma (5)
- Jeffery Wilson, RB, Elkhart, 6-0, 190 — offers from New Mexico State, North Texas, Texas State, ULM (4)


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METAL/NONMETAL MINE FATALITY - On January 8, 1997, a mechanic with approximately 3 years of mining experience was killed at a sand and gravel operation. While performing mechanical repairs on a wash plant blade mill, the mill became energized pulling the victim into it. He died from crushing injuries. This is the first fatality reported in calendar year 1997 in the metal and nonmetal mining industries. As of this date in 1996, there were no fatalities reported in these industries. This fatality has been classified as machinery.
For more information:
MSHA's Fatal Accident Investigation Report [FTL97M01]


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Temperature Map
The unofficial end of summer – Labor Day weekend – concludes in summer-like style with toasty conditions and daily storm opportunities. The best chance of storms is on Labor Day itself.
Through Tonight: No weather concerns whatsoever. It's partly cloudy to mostly clear, and a bit humid. Overnight lows range from the low 70s downtown to the upper 60s in the cooler suburbs. Light winds from the south at 5-10 mph.
Saturday through Sunday: Both days are partly to mostly cloudy, very warm and humid, with a 30-40 percent chance of afternoon and evening showers and storms. On Saturday, the highest rain chances are north and west of the District. On Sunday, the chances are pretty uniform throughout the region.
To be sure, it's dry most of the time for outdoor activities, but with dew points in the sticky upper 60s and 70s (Sunday), you'll want to take it easy. High both weekend days are in the mid-to-upper 80s. Sunday morning's low temperatures range from 70-75 (suburbs-city). Winds tend to be from the south around 10 mph.
Labor Day (Monday): Very warm and humid with a 50 percent chance of afternoon and evening showers/storms thanks to an approaching fall-like front. Highs are again in the mid-to-upper 80s.
See Camden Walker's forecast into early next week. And if you haven't already, join us on Facebook and follow us on Twitter . For related traffic news, check out Dr. Gridlock.
Pollen: Tree counts are LOW, grasses are MODERATE, weeds are MODERATE-HIGH, mold spores are LOW-MODERATE.
Accuweather's fall foliage outlook: "… this year's most vibrant display of foliage will occur across the mid-Atlantic," Accuweather forecasts. It says the near normal temperatures and precipitation it predicts will help bring out the best of the season's color.


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Over time, wine has seen some not so desirable incarnations that infringed on its classy reputation. First there was Thunderbird and Night Train, then there was the boxed wine revolution, and now there's the wine sippy cup, called "Vino2Go".
The Vino2Go™ wine sippy cup tumbler is the simplicity of style, an active wine lovers dream come true. This BPA-free durable drink-ware has a familiar wine glass shape making it a convenient and easy to use product. You get your choice of 5 different colored tops: Merlot Red, Verde Green, Clear Ice, Party Pink & Business Black! The wine tumbler holds 10 oz of wine – standard wine glass size.
Still not sure which is a worse idea – encouraging drinking while on the go or putting booze in the type of cup typically used by toddlers.
Product Page: ($16 via Geekologie)


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Be Homer's Valentine!
By Charlotte Knights | February 3, 2014 12:00 PM ET
(Uptown Charlotte, NC) -- Valentine's Day is just around the corner and the Charlotte Knights are spreading love around town with help from one of their romantic mascots. This year, the Knights are giving fans a chance to surprise their sweetie with a special Valentine's Day delivery from the team's lovable mascot Homer the Dragon.
For $75, Knights fans can purchase a special Valentine's Day package that will send Homer door-to-door with two field box vouchers to a Knights game at BB&T Ballpark this season, a rose, and either a Knights hat or Knights t-shirt. Homer will also happily take a photo with the lucky Valentine's Day recipient and that photo will be emailed to the lucky sweetheart later in the day. Homer will deliver the special gifts on either Thursday, February 13 or Friday, February 14 (Valentine's Day). Fans will have the option of choosing which game they would like to attend (April 22 or May 5).
$75 Package Includes:
- 2 Field box vouchers (good for Tuesday, April 22nd or Monday, May 5th)
- A Knights hat or shirt
- Photo with Homer
- Single Rose
All orders can be placed online at www.charlotteknights.com. Shortly after your order is placed, you will be contacted by a Charlotte Knights representative to discuss all of the details. Quantities are limited and are filled on a first-come, first-serve basis. Orders will be taken through Monday, February 10. All orders must be within a 15-mile radius of BB&T Ballpark in Uptown Charlotte.
The Knights will open BB&T Ballpark on Friday night, April 11 at 7:05 p.m. against the Norfolk Tides. For more information about Charlotte Knights Baseball, log on to www.charlotteknights.com. Uptown Knights are Better Than Ever!
This story was not subject to the approval of the National Association of Professional Baseball Leagues or its clubs.View More