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Chantilly Neighborhood
Chantilly, the neighborhood pocketed between Elizabeth and Plaza Midwood, is one of my favorite neighborhoods in Charlotte. Ok, so I live there and I am obviously a little bias, but I will give you the top 5 reasons why I love it and you would too:
1) Beautiful huge oak trees line the streets of Chantilly. Taking a jog or walk thru the neighborhood during the summer heat you will stay mostly shaded, which means we also have a very colorful fall and a gorgeous green spring!
2) Walking distance to not only Elizabeths business district, but also Plaza Midwoods. Chantilly gets the best of Elizabeth and Midwood since we are nestled right between the two and if you dont know some of the best mom and pop restaurants in Charlotte are in these two neighborhoods.
3) No one house is the same and with most of the homes being built post WWII they were nothing more the 2 bed 1 bath homesmuch has changed! Weve built up, expanded out, added front porches, updated kitchens and baths, or in some cases torn down existing homes to build brand new ones this is why we are all extremely unique!
4) Neighbors My husband and I have been in the neighborhood for four years and some of our closest friends are now right down the street. We have met our Chantilly friends at the park with Bishop (our German Shepard), at a neighborhood social or picnic, and some we met at each others houses. Regardless of how we met them, we dont walk too far to hang out with the gang these days!
5) The parks We have a great swing set and jungle gym at our Chantilly Park, which is often covered with little peeps climbing, swinging, or chasing each other. Chantilly is also dog-loving neighborhood. Rarely will your K9 be the only one of his/her kind there.
If you dont know about Chantilly and youre in the Plaza Midwood or Elizabeth areastake a drive through our streets, you wont be disappointed!
Thinking of selling your home in Chantilly?
Find out what your home might be worth at:
2336 Kingsbury Drive
Subdivision: Chantilly
|Back to Property List
|New Construction in Chantilly, designed and built by THR Design Build. Spacious and open design with 9' ceilings on 1st and 2nd floors. Beautiful site-finished hardwoods throughout house. Large master suite with beautiful bath and huge walk-in closet. 507 sf of finished basement w/ full BA. Option to finish more.
Basic Information
Contact An Agent:
|Additional Photos:
Listing courtesy of: Dwight Brokerage
The data relating to real estate on this Web site derive in part from the Carolina Multiple Listing Services, Inc. IDX program. Brokers make an effort to deliver accurate information, but buyers should independently verify any information on which they will rely in a transaction. All properties are subject to prior sale, change or withdrawal. Neither Genevieve Tisdelle Williams, Broker nor any listing broker shall be responsible for any typographical errors, misinformation, or misprints, and they shall be held totally harmless from any damages arising from reliance upon this data. This data is provided exclusively for consumer -- personal, non-commercial use and may not be used for any purpose other than to identify prospective properties they may be interested in purchasing. © 2017 Carolina Multiple Listing Services, Inc.


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GENEALOGY-DNA-L ArchivesArchiver > GENEALOGY-DNA > 2006-05 > 1148090948
From: "John McEwan" <>
Subject: R1b SNP page updated
Date: Sat, 20 May 2006 14:09:08 +1200
In-Reply-To: X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2869
Dear List
The R1b SNP page has been updated.
New aspects
* 7 new entries and updates
* Newly designed R1b SNP page. I took Dennis Wrights advice (and html
code) and made the page have two frames to provide a fixed heading and
scrolling data area. It is years since I hand coded html pages.
Hopefully: you can see the page if your browser supports frames, and the
links should all work and start up a new browser window.
* At the bottom in "squinty font" is a summary of how many individuals
have been tested for a SNP, how many are positive, and percentage
New data
* As has been reported on the list we now have 2 S21+, S29+ individuals
aka R1b1c9b. These individuals have a genetic distance of 8 at 37
markers (8 mismatches). This indicates that this is not a recent private
SNP but is better defined as a prehistoric clan SNP. In conjunction with
a total of 38 public SNP results in R1b this SNP now has reached
confirmed status according to the new ISOGG SNP tree criteria. See
www.isogg.org/tree. Both members are in R1bSTR3 haplotype cluster or as
Ken Nordtvedt defines it R1b-F2. For other members of this haplotype
cluster see http://www.geocities.com/mcewanjc/37strallhapr1bone.pdf
Please note some members of R1bSTR3 are not in this S29+ so even at 37
STR markers this group cannot be clearly distinguished. Now that the SNP
has been confirmed, individuals who are within this cluster or are close
matches to the two tested positive are encouraged to test. If we get
sufficient positives its origin and geographical distribution can be
better resolved.
* As has been reported to the list we now have 2 confirmed S21+, S26+
individuals aka R1b1c9a. These individuals have a genetic distance of 7
on 25 markers and 7 marker mismatches. This indicates that this is not a
recent private SNP but is better defined as a prehistoric clan SNP. This
SNP also called L1 or Little SNP is associated with a null at DYS439.
Based on individuals who have a null value at this marker quite a bit is
known about its origin and distribution. See
for further information. These individuals are often associated with
R1bSTR32 and associated clusters. In conjunction with a total of 43
public SNP test results in R1b, S26 now has reached confirmed status
according to the new ISOGG SNP tree criteria. I will leave it to Neal
Fox and Leo Little for detailed comment on the challenges awaiting
further exploration of this group. To me it seems to be determining if
it is present/originated in continental Europe and further defining its
geographical spread and surname diversity within England. Again this
will give clues to its origin.
* Just a small observation about the M222+ cluster, Some of these
members are a long way from the R1b modal and in fact they are nearly as
close to the R1a modal as the R1b modal.
* We also have a new entry Best, who is markedly distant from the
existing R1b STR clusters I have previously defined. As time goes on
these unusual haplotypes continue to appear.
Finally as usual if you note any errors or new results please contact
me. I know there are quite a few people who have SNP results who are not
on this list (mainly M222+ individuals) and I would encourage them to
contact me as the information provided helps all those interested in
their origins.
John McEwan
-----Original Message-----
From: Jason S. Clary [mailto:]
Sent: Friday, 19 May 2006 6:00 a.m.
To: John McEwan
Subject: Re: [DNA] R1b SNP page updated
Er.. that last one is SHHB7 not SSHB7.
----- Original Message -----
From: "Jason S. Clary" <>
To: "John McEwan" <>
Sent: Thursday, May 18, 2006 10:53 AM
Subject: Re: [DNA] R1b SNP page updated
> Maybe.. That's not where I got em, though... I found em poking around
> various public projects while looking for matches for someone in my
> Hmm.. small nuff group to look up if you know the trick:
> looks like Vanover isn't but the rest are.
> This data should be more reliable. It's cut-n-paste from FTDNA
> Some of those haven't been updated and one says he's haplotype H for
> reason.
> Anyway.. they all have "green" R1b1c7 on FTDNA.
> ----- Original Message -----
> From: "John McEwan" <>
> To: "'Jason S. Clary'" <>
> Sent: Thursday, May 18, 2006 4:23 AM
> Subject: RE: [DNA] R1b SNP page updated
>> Are these people listed in FTDNA or in Ysearch?
>> -----Original Message-----
>> From: Jason S. Clary [mailto:]
>> Sent: Tuesday, 16 May 2006 12:27 p.m.
>> To: John McEwan
>> Subject: Re: [DNA] R1b SNP page updated
>> These may or may not duplicate data you already have but I thought
>> send
>> em over in case they are useful to you.
>> These are FTDNA confirmed M222+ individuals I've located.
This thread:
|R1b SNP page updated by "John McEwan" <>

subset: commoncrawl


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IT IS believed that Judy Moran was more worried about losing the estimated $350,000 proceeds left from the sale of her Ascot Vale house than how long she would spend in prison for murder.
Sentenced two weeks ago to 26 years' jail with a minimum of 21 years for murdering brother-in-law Des ''Tuppence'' Moran, she was comforted when Justice Lex Lasry dismissed an application to forfeit as tainted property the balance of the house's $1.07 million sale proceeds.
But Victoria's Director of Public Prosecutions (DPP) will now appeal against Justice Lasry's ruling. The DPP is expected to file appeal papers in the Court of Appeal seeking to overturn the decision, presumably on the basis Justice Lasry erred in law.
He told Moran, 67, who contracted Geoffrey Armour to kill Mr Moran with another man, that she planned the crime for financial gain or over hostility to him or a combination of both.
The DPP submitted that Moran's house proceeds should be forfeited under confiscation law as the property was used in ''connection'' with the commission of murder.
It was argued that the house was used as a base for the murder, items used in the crime - the murder weapon, clothes and a wig - were stored and concealed there in a safe, and a car driven to and from the crime scene was stored at the property, and it provided security for a loan used partly to pay or reward Armour.
In opposing the application, barrister John Saunders said the proceeds were Moran's only remaining assets for (prison) life's necessities and for use if she lived to be paroled.
He argued the property was not tainted within the meaning of the Confiscation Act.
Justice Lasry said he believed it was ''nothing more than speculation'' and not supported by evidence to conclude the property was used as a base ''from which the murder would have been planned and discussed''.
He also found the DPP's four other grounds were not sufficient to taint the property.
When contacted by The Age, Moran's lawyer Jim Stavris yesterday confirmed he had been notified that the DPP would appeal and that his client would defend it.


text:
Relative to the way they began the season, the Memphis Grizzlies are really struggling right now. They've dropped four of their last six, with Friday's defeat being one of their most frustrating thus far. In an 86-84 loss to the Blazers, Mike Conley missed a point-blank layup that would've given the Grizzlies a one-point lead with just seven seconds to go. It was Memphis's ninth consecutive miss, and Rudy Gay's last-ditch jumper would be number ten. Ten straight misses to close out a two-point loss. Pretty discouraging. How 'bout Marc Gasol, though? With his career-best eight blocked shots, the big Spaniard tied his brother Pau's Grizzlies franchise record. As cool as it as that two brothers are now officially tied for an NBA franchise record, this story gets even cooler. At least I think it does. Pau, you see, did not hold this record alone. He shared it with none other than Stromile Swift, who swatted eight 76er shots on November 20th, 2003. Swift added 12 points and 16 rebounds to his impressive block total, and I think it's safe to assume he was much more spectacular in his setting of this record than either of the Gasol brothers were. Thank you, Marc Gasol, for bringing about a Stromile Swift reference during an NBA basketball game. Also, thank you for not surpassing his record. He doesn't have many.
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text:
Italian Couscous with Parmesan and Herbs
Chewy Italian couscous, also called freula, is a Sardinian-style toasted semolina pasta. It comes in small, medium and large grains and is available at specialty food stores. Any small pasta, such as ditalini, orzo or acini de pepe, makes a good alternative to medium-grain freula.
Slideshow: Fast Weekday Pastas
- Servings: 8
- 1 pound medium-grain Italian couscous (about 2 1/2 cups)
- 4 tablespoons unsalted butter, cut into 1/2-inch pieces
- 1/4 cup freshly grated Parmesan cheese
- 2 tablespoons finely chopped flat-leaf parsley
- Salt and freshly ground pepper
How to make this recipe
Bring a large pot to salted water to a boil. Add the Italian couscous and cook over moderately high heat, stirring occasionally, until tender but still chewy, about 20 minutes. Drain well and return the Italian couscous to the pot. Add the butter and Parmesan and stir over low heat until melted. Add the parsley, season with salt and pepper and serve.


text:
1 Recommendation
chad's recommendation
I've been a long time of Jeff Tweedy and Wilco, in general. When their most recent album came out I was skeptical to say the least. However, I was quickly caught up in the new sound. The highlights come with "Hate it Here" and "Walken."
My Collections:
This CD is soo good-we LOVE it and Wilco in general. It is a must own...
- mrsotis


text:
Avea, one of Turkeys leading mobile operator sees a boost of 2.5 million new subscribers with the introduction MNP. Avea predicts that nearly 9 million mobile users in Turkey will switch their operators, taking advantage of this new service.
Source: Wireless Federation.
© Copyright 2017, ITU
The information presented within this blog comes from various organizations around the world. ITU encourages users to seek more detailed information from the original source through the links provided.
Links to third-party websites are provided for the convenience of all users. The ITU is not responsible for the accuracy, currency or the reliability of the content on these third-party websites. ITU does not offer any guarantee in that regard nor does ITU endorse the third-party organizations, their sites or content.


text:
Diane Hine
Diane Hine (25 July 1956)
» Click to list general statistics » OR, select a date :
Fruit Fly Tiff (Dodecatina Refrain)
F or
R easons
U nclear he
I mbibes more when
read more >>
- Hits: Number of visitors who read the poem on the given date.
- Posts: Number of visitors who posted the poem to a friend (via e-mail) on the given date.
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text: Kohlberg Kravis Roberts & Co. L.P., a leading global investment firm, today announced the signing of a definitive agreement under which funds advised or controlled by KKR are acquiring a significant minority stake in RigNet, Inc. (NASDAQ: RNET) from Cubera, an investment firm specializing in the Nordic private equity market. Upon closing of the acquisition of 4.75 million shares, which is subject to customary approvals, KKR will become RigNet's largest shareholder, holding a 27% stake in the company. RigNet is a leading provider of managed remote communications solutions, systems integration and collaborative applications to the global upstream energy sector. Operating across the life of the field, RigNet serves offshore and onshore drilling rigs, production facilities and energy maritime with solutions ranging from fully-managed voice and data networks to more advanced applications that include video conferencing and real-time data services. It provides services to over 1,100 remote sites in over thirty countries across six continents. Johannes Huth, Member of KKR and Head of Europe, stated: "We are investing in a fast-growing global market leader that has created an attractive niche at the intersection of three of our core investment themes: energy, technology, and services. All three sectors exhibit structural growth well ahead of broader GDP trends. We are excited about this addition to our investment portfolio and we look forward to supporting RigNet's growth strategy as a patient and constructive shareholder." Mattia Caprioli, Member of KKR and Head of KKR's services sector team in Europe, added, "We believe that the digitalization of upstream energy has only just begun. A prime example is the rapidly growing demand for broadband connectivity for oil and gas operations in remote and harsh environments. Today, the connection speed of deepwater drilling rigs is a fraction of the internet connection speed of a typical family home. Scarce bandwidth needs to be shared between regulatory supervision, remote management, firm-wide ERP systems and business communication. Crew well-being is increasingly tied to connectivity as employees stay in touch with their families, use telemedicine services and use web and intranet as a source of infotainment and training. As significantly more bandwidth capacity becomes available and more real-time information is shared between rigs and headquarters, drillers, operators and oil field services providers will be able to improve health and safety, drive operational efficiencies and boost crew morale. We expect RigNet's mission-critical connectivity, integrated systems and innovative services to play a vital role in helping customers shape the digital oil field of the future. We share management's goal to establish RigNet as a pre-eminent global services provider to the oil and gas industry."


text:
This is the second annual Financial Times 300 list, produced independently by the Financial Times in collaboration with Ignites Research, a subsidiary of the Financial Times that provides business intelligence on the investment management industry. More than 2,000 elite Registered Investment Advisory (RIA) firms were invited to apply for consideration, based on their assets under management (AUM). The 630 RIA firms that applied were then graded on six criteria: AUM; AUM growth rate; years in existence; advanced industry credentials; online accessibility; and compliance records. Please see important disclosure.
Concise and accurate information inform our superior service.
We're prepared for performance at the highest level.
We see our strategies through with focus and consistency.


text:
Today is Thursday, Feb. 14, the 45th day of 2013. There are 320 days left in the year. This is Valentine's Day.
Today's highlight:
On Feb. 14, 1963, Federico Fellini's art-house classic "8½," a movie about a movie director played by Marcello Mastroianni, was first released in Italy.
On this date:
In 1778, the American ship Ranger carried the recently adopted Stars and Stripes to a foreign port for the first time as it arrived in France.
In 1859, Oregon was admitted to the Union as the 33rd state.
In 1895, Oscar Wilde's final play, "The Importance of Being Earnest," opened at the St. James's Theatre in London.
In 1903, the Department of Commerce and Labor was established. (It was divided into separate departments of Commerce and Labor in 1913.)
In 1912, Arizona became the 48th state of the Union as President William Howard Taft signed a proclamation.
In 1913, labor leader Jimmy Hoffa was born in Brazil, Ind.; college football coach Woody Hayes was born in Clifton, Ohio.
In 1920, the League of Women Voters was founded in Chicago; its first president was Maud Wood Park.
In 1929, the "St. Valentine's Day Massacre" took place in a Chicago garage as seven rivals of Al Capone's gang were gunned down.
In 1949, Israel's Knesset convened for the first time.
In 1979, Adolph Dubs, the U.S. ambassador to Afghanistan, was kidnapped in Kabul by Muslim extremists and killed in a shootout between his abductors and police.
In 1988, Broadway composer Frederick Loewe, who wrote the scores for "My Fair Lady" and "Camelot," died in Palm Springs, Calif., at age 86.
In 1989, Iran's Ayatollah Khomeini called on Muslims to kill Salman Rushdie, author of "The Satanic Verses," a novel condemned as blasphemous.
Associated Press


text:
Wednesday, October 31, 2007
The Tipping Point
To their credit, leading Democrats are now dropping support of Mukasey one by one. By why is this still a partisan issue? This is an issue about who we are-- about the core character of America.
Former SERE trainer Malcolm W. Nance has just penned what I believe to be the most informed, insightful, and empasioned account of the waterboarding controversy to date. He writes:
Now American use of the waterboard as an interrogation tool has assuredly guaranteed that our service members and agents who are captured or detained by future enemies will be subject to it as part of the most routine interrogations. Forget threats, poor food, the occasional face slap and sexual assaults. This was not a dignified 'taking off the gloves'; this was descending to the level of our opposition in an equally brutish and ugly way. Waterboarding will be one our future enemy's go-to techniques because we took the gloves off to brutal interrogation. Now our enemies will take the gloves off and thank us for it.Read the whole piece, and read it again. We are now at the tipping point. Which path will we choose to take?
There may never again be a chance that Americans will benefit from the shield of outrage and public opinion when our future enemy uses of torture. Brutal interrogation, flash murder and extreme humiliation of American citizens, agents and members of the armed forces may now be guaranteed because we have mindlessly, but happily, broken the seal on the Pandora's box of indignity, cruelty and hatred in the name of protecting America. To defeat Bin Laden many in this administration have openly embraced the methods of by Hitler, Pinochet, Pol Pot, Galtieri and Saddam Hussein.


text:
03-28-2008 07:43 AM - last edited on 03-28-2008 09:27 AM by JaneL
03-31-2008 07:00 AM - last edited on 03-31-2008 12:01 PM by andyP
Note from Moderator: Please update your profile with your correct country location as per the forum rules. Products, options and services vary from market to market. Knowing your location helps us help you.
Message Edited by D-art on 03-31-2008 04:01 PM
Message Edited by D-art on 03-31-2008 04:01 PM
i too was experiencing this message.
I downloaded all of the drivers via Update Retriever (UR) to my network share/repository. I then used System Update (SU) to point to the share to download the files. I received a login prompt (as usual) and then it would give the message that there were no updates for my system.
I finally figured out that inside the UR, the default setting for my options were "TEST" and not "ACTIVE". Switching these to ACTIVE in the UR fixed *MOST* of these issues.
I am now just having issues where the a few of the updates are not being displayed. I opened up the XML file in the repository and verified the system type was correct for that download (example: video drivers for that model).
if i point the SU program to Lenovo for updates, it finds all of the required drivers.
Any suggestions to why it finds some drivers and not others?
after hours of testing, i believe that i have figured out what is going on. the xml file included with the driver specifically shows xp pro sp1 and sp2 support but doesn't mention sp3 which we are running. if i remove sp3, it finds the driver... if i reapply sp3, the driver is no longer available in the list.
is there anyone from lenovo available to comment on this at all?
NVision wrote:
is there anyone from lenovo available to comment on this at all?
Welcome to the forum!
There are a few Lenovo employees roaming around in here helping out, but this is not an official support channel. You may or may not get an answer directly from one of them as there are way more of us than them and all of them, even Mark, have full-time jobs doing other things. Instead, this is designed primarily to be a user-to-user support forum. If you require immediate assistance, please call the support line.
English Community Deutsche Community Comunidad en Español Русскоязычное Сообщество
2015 X1 Carbon, ThinkPad Slate, T410s, X301, X200 Tablet, T60p, HP TouchPad, iPad Air 2, iPhone 5S, IdeaTab A2107A, Yoga Tablet, Yoga 3 Pro
I AM one of those crazy ThinkPad zealots!
If you find a post helpful and it answers your question, please mark it as an "Accepted Solution"!
I am not a Lenovo employee


text:
Revisiting Rail, Civil Unions
Wednesday - June 23, 2010
So, what are we going to be talking about during the four-and-a-half long months remaining in this already oh-so-long special, primary and general election year?
Sadly, much of the heat will be generated by topics we've been discussing ad nauseam for decades, starting with a fixed-rail mass transit campaign for Honolulu.
It appeared to have been settled in 2008 when Oahu residents voted 156,051 to 140,818 for the project. They also re-elected the rail project's champion of the moment, Mufi Hannemann. He stood in a long line of rail champions: every Honolulu mayor save one since 1968.
So rail's a done deal, right? Of course not. Honolulu east of Red Hill and Windward Oahu made up the bulk of those 140,818 anti-rail votes, and many of those folks will not let the issue die.
Perhaps they shouldn't. Any project with a $5.5 billion price tag needs the voters' continued attention.
They will give it in the months ahead as University of Hawaii engineering professor Panos Prevedouros takes the anti-rail, pro-high occupancy transit position into the winner-take-all mayoral election. Prevedouros may very well win it. He's a quick-witted, excellent debater with a disarming sense of humor. He proved that in the 2008 mayoral primary. And he's the only one of the five major announced mayoral candidates who opposes the proposed mass transit system. The others - city Prosecutor Peter Carlisle, city managing director Kirk Caldwell and City Councilmen Donovan Dela Cruz and Rod Tam - all have their strengths, experience in county government and name recognition foremost among them.
But Prevedouros has a pool of 140,818 opponents of rail in which to troll for votes. That's a big pool, and there aren't any other mayoral boats with lines in the water.
Transit also will be prominent among the issues dominating the gubernatorial debate. Indeed, it already is. Neil Abercrombie joins the mayor in supporting rail, but the former U.S. representative has been criticizing Hannemann for months. The mayor, he argues, has moved too slowly and now wants to walk away from the project before construction has begun. Hannemann responds that it's Republican Gov. Linda Lingle's fault, that her stubbornness has caused the delays.
And so it goes.
Then there are civil unions, an issue that we've been debating for almost two decades. The Hawaii Supreme Court launched the debate in the early 1990s by deciding in favor of same-sex marriages. Opponents succeeded in tying the Legislature in knots for several sessions and getting a constitutional amendment on the ballot that defined marriage as between a man and a woman. The voters approved it, and there the issue has percolated.
Whether Lingle signs, vetoes or lets the civil unions bill become law without her signature, the issue will play a central role in the primary and general elections. Both Duke Aiona and Lynn Finnegan, the most likely Republican ticket in the fall, oppose civil unions. Their positions will energize the thousands of demonstrators opposed to the bill to campaign for and vote the Republican ticket in November.
But in the primary elections, anti-civil union voters may find themselves choosing the Democrats' ballot. There they'll find Hannemann, who opposes civil unions, while Abercrombie joins the legislative Democrats who supported them. Among the Democrats running for lieutenant governor, both Sens. Bobby Bunda and Norman Sakamoto voted against the civil unions bill.
So we'll redo both issues.
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text:
Received this via e-mail...make sense to you?
Free people are not equal. Equal people are not free.
"A gun is like a parachute. If you need one, and don't have one, you'll probably never need one again."
The definition of the word Conundrum is: something that is puzzling or confusing.
Here are six Conundrums of socialism in the United States of America:
1. America is capitalist and greedy - yet half of the population is subsidized.
2. Half of the population is subsidized - yet they think they are victims.
3. They think they are victims - yet their representatives run the government.
4. Their representatives run the government - yet the poor keep getting poorer.
5. The poor keep getting poorer - yet they have things that people in other countries only dream about.
6. They have things that people in other countries only dream about - yet they want America to be more like those other countries.


text:
Guard your steps when you go to the house of God. Go near to listen rather than to offer the sacrifice of fools, who do not know that they do wrong. Do not be quick with your mouth, do not be hasty in your heart to utter anything before God. God is in heaven and [Read More…]
{Practicing Benedict} When it is best not to speak
{Practicing Benedict} The God who comes
Welcome to Mama:Monk's weekly Wednesday series examining St. Benedict's Rule and what it has meant to me as a stay at home mom. (Full disclosure! I'm no expert on Benedict or the Benedictine Order. I just love him/them and can't seem to stop reading books that have "Benedictine" in the title.) In my former life I traveled [Read More…]
This Way to Austin…
I know, I know. How clichéd is that picture? But I took it myself. I passed that sign twice every Tuesday on Franklin Street in SF, on my way to drop off and pick up August from his school. I never noticed it, of course, until Chris' interview for the job in Austin. After that, [Read More…]
Mama admits she's kind of a crazy person.
We all have our crazy-makers. Mine is email, and phone calls, and thank you notes—basically any communication that I'm expected to follow through on. Sometimes I do alright with those things. I make lists. I set goals: Return four emails before bed! But sometimes I torture myself. My deepest weakness is a longing to please [Read More…]
Sisyphus and Motherhood and Coffee
Yesterday morning I woke to a quiet (and sort of creepy) almost three-year-old standing beside my bed at 6:15, silently staring at me. Good morning, Wednesday. After I got August settled and watching a Thomas show, I moved toward my coffee pot, only to have Brooks begin crying and crying. I bounced him in the [Read More…]
On Ripeness and Courage
Why does "full term" have to feel so…full? I once read a lovely book (which I really ought to read again…and you should read as well) called Great With Child, in which the author (Debra Rienstra) refers to herself as "ripe" at the end of her pregnancy. I keep thinking about that. I feel ripe…teetering [Read More…]


text:
Posted Mon, February 13, 2012
Celebrating over ten years of professional helicopter pilot training, Helicentre Aviation Ltd. are excited to announce the launch of two funded professional helicopter pilot training scholarships.
Training from zero experience to working Commercial Helicopter Pilot is what Helicentre Aviation has specailsed in for over a decade.
Helicopter pilots have self funded their careers in to the civilian rotary world since the introduction of civilian helicopters in the 1950's - never before has a helicopter flying scholarship of this calibre been open to people looking to change their career, until now!
Once awarded the scholarships will cover in-house professional training and hour building with a total estimated value of up to £67,000.
Applicants who wish to know more about the scholarships can register their interest by filling out the online form.
Further information, terms and conditions and application forms for the Commercial Helicopter Pilot and Helicopter Flight Instructor scholarship programmes will be released on 1st March 2012.
To stay up to date with he latest news visit our scholarships page and register your interest today.
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text:
Hello, my name is Xavier.
I'm from Barcelona, Catalonia.
I'm just testing mon0wall 1.2 on a s-epia hardware and I've realized
that only 16 concurrent pptp connections are allowed.
Reading the faq i've finished on chri's page, with useful information
about this 'issue'.
So, I tryed using the hidden exec.php page to download the file
/etc/inc/globals.inc and modifying the parameters he gives
"n_pptp_units" => 50,
"pptp_subnet" => 26,
Then upload the file again at /tmp/globals.inc and copying it at the
original location.
It seems to work, but when I've rebooted, it shows again 16 maximum
concurrent connections.
I think that this is a right way to do this hack, but I suppose I've
missed some step.
Could u help me?
Thanks in advance
Best regards
Xavier Sanz


text:
Oil Mist Lubrication: Practical Applications
Oil mist lubrication is used on literaily thousands of pumps and electric motors in the U.S. petrochemical industry. This lubrication method has been demonstrated to reduce rolling element bearing failures by as much as 90%, generating significant technical and economic advantages over other lubrication systems. Here is the complete explanatory guide to this modern reliability and profitability improvement approach. The comprehensive text explains how extensive research at Texas A&M University in the late 1980's and early 1990's has solidified our understanding of the underlying reasons why and how oil mist actually works in the safest, most reliable, and cost-effective fashion.
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text:
Quantitative polymerase chain reaction analysis of AQP4 mRNA expression in 3 BL6 mice using SYBR-Green–based (Invitrogen) chemical analysis (A) and 2 human control tissue samples using SYBR-Green–based and gene expression assay (TaqMan; ABI) chemical analysis (B). Tissues analyzed were liver (L), kidney (K), muscle (M), frontal lobe (Br), brainstem (BS), hypothalamus (HyT), spinal cord (SC), and optic nerve (ON). Results are expressed as a mean ratio of the samples tested relative to a reference gene, hypoxanthine guanine phosphoribosyl transferase 1 (HPRT). In mouse tissue, M1 and M23 AQP4 isoforms were evaluated separately; in human tissue, total AQP4 (M1 and M23 isoforms) mRNA was evaluated. SYBR-Green–based and TaqMan chemical analysis methods show quantitatively different but similar relative levels of expression.
Analysis of AQP4 protein expression by sodium dodecyl sulfate–18% polyacrylamide gel electrophoresis in neuromyelitis optica–relevant tissues from representative mouse (A), rat (B), and human (C) tissues. Proteins analyzed are from the cerebral hemisphere (Br), brainstem (BS), optic nerve (ON), spinal cord (SC), kidney (K), and liver (L). The M1 and M23 lanes were loaded with proteins isolated from HEK293 cells transfected separately with human M1 or M23 AQP4 isoform pcDNA. Loading was optimized to distinguish M1 from M23; differences in β-actin reflect the differences in protein loading. Densitometry results are summarized in the graphs to the right of the gels.
Analysis of protein aggregation by blue native polyacrylamide gel electrophoresis in CNS tissue from a BL6 mouse (A), Lewis rat (B), and human control (C). Gels were loaded to optimize the visualization of upper-order aggregates. Proteins analyzed are from the cerebral hemisphere (Br), spinal cord (SC), and optic nerve (ON). Protein molecular weights correspond to a protein standard designed for molecular weight estimation of aggregated proteins (NativeMark Protein Standard; Invitrogen).
A, Blue native polyacrylamide gel electrophoresis (BN-PAGE) comparing aggregated AQP4 in ON vs Br tissue. Protein molecular weights correspond to a protein standard designed for molecular weight estimation of aggregated proteins (NativeMark Protein Standard; Invitrogen). B, Two-dimensional protein analysis wherein lanes from a 3% to 12% nondenaturing BN-PAGE gel are cut and electrophoresed in a second dimension on a denaturing sodium dodecyl sulfate–12% polyacrylamide gel (12% SDS-PAGE). The long horizontal arrow is in the direction of electrophoresis for the single-dimension 3% to 12% BN-PAGE. The vertical arrow is in the direction of electrophoresis for the second-dimension 12% SDS-PAGE. The short horizontal arrows indicate the positions of M1 and M23 isoforms of AQP4 protein.
Comparison of aquaporin 4 (AQP4) mRNA (A), protein (B), and protein aggregation (single-dimension blue native polyacrylamide gel electrophoresis [BN-PAGE] [C] and 2-dimensional BN-PAGE [D]) between a single patient with NMO and human control samples. The optic nerve (ON) was studied using the SYBR-Green (Invitrogen) technique for mRNA expression from a single patient with NMO and from 5 control ONs (A) and from a single control (B through D) in cerebral hemisphere (Br), spinal cord (SC), ON, and kidney (K) for protein expression and aggregation. Messenger RNA expression is intermediate in NMO. Sample loading was performed to optimize the distinction of M1 from M23; β-actin serves as a comparator protein to control for differences in protein loading. Protein molecular weights (C) correspond to a protein standard designed for molecular weight estimation of aggregated proteins (NativeMark Protein Standard; Invitrogen). 12% SDS-PAGE indicates sodium dodecyl sulfate–12% polyacrylamide gel. Arrows are explained in the legend to Figure 4.
Matiello M, Schaefer-Klein J, Sun D, Weinshenker BG. Aquaporin 4 Expression and Tissue Susceptibility to Neuromyelitis Optica. JAMA Neurol. 2013;70(9):1118-1125. doi:10.1001/jamaneurol.2013.3124
© 2017
To understand the predilection for optic nerve and spinal cord pathologic changes in neuromyelitis optica (NMO).
To evaluate tissue-specific expression (RNA and protein) and supramolecular aggregation of aquaporin 4 (AQP4) in mouse, rat, and human tissues.
Quantitative polymerase chain reaction, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and blue native polyacrylamide gel electrophoresis.
Laboratory analysis of mouse, rat, and human tissue.
Tissues from control individuals and 1 patient with NMO obtained at autopsy.
Neuromyelitis optica in the patient.
Main Outcomes and Measures
Tissue-specific messenger RNA and protein expression and supramolecular aggregation of AQP4.
The AQP4 messenger RNA and proteins were much more highly expressed in the optic nerve and spinal cord relative to other regions of the brain and to non–central nervous system tissues in all species evaluated. Large supramolecular aggregates of AQP4 were overrepresented in the optic nerve and spinal cord relative to other central nervous system tissue. There was no difference in AQP4 expression between an individual with NMO and the control samples.
Conclusions and Relevance
Optic nerve tissue from an individual with NMO did not differ in AQP4 expression from control samples. The relatively high levels of expression and of supramolecular aggregation in the optic nerve and spinal cord may contribute to the predilection of these structures to NMO pathologic changes.
Neuromyelitis optica (NMO) is an autoimmune, relapsing inflammatory disease of the central nervous system (CNS) with a predilection for optic neuritis and myelitis associated with long spinal cord lesions.1 Other brain structures with high levels of aquaporin 4 (AQP4) immunoreactivity are also preferentially targeted.2 A pathogenic autoantibody specific for extracellular epitopes of the transmembrane CNS-predominant water channel protein, AQP4 is detectable in most patients.3 This antibody reproduces many aspects of NMO-specific pathologic changes when injected directly into the CNS in the presence of human complement or when injected intravenously in the setting of a disrupted blood-brain barrier resulting from prior induction of experimental autoimmune encephalomyelitis or injection of a Freund adjuvant.4- 7 The relative predilection of this disease for the optic nerve and spinal cord remains poorly understood, as are the reasons why other AQP4-expressing tissues are not targeted.
Aquaporin 4 protein is expressed in humans as 2 isoforms that result from alternative transcription initiation and alternative splicing of the same gene encoded on chromosome 18. M23, the shorter isoform, lacks 22 N-terminus amino acid residues of the longer M1 isoform.8 The basic unit of AQP4 assembly is a heterotetramer. Heterotetramers with a high proportion of M23 isoform aggregate as supramolecular complexes9,10 that lead to formation of orthogonal arrays of particles (OAPs) on the surface of cells detectable by freeze-fracture electron microscopy.11
A recent analysis of expression of M1 and M23 isoforms using quantitative polymerase chain reaction (PCR) and rapid amplification of complementary DNA ends (RACE) reported a relatively high proportion of M1 isoform–specific RNA (nonaggregating isoform) in tissues susceptible to NMO and that M1 expression might be the critical determinant of susceptibility of certain tissues to NMO.12 Neither AQP4 protein isoform-specific expression nor supramolecular aggregation was analyzed in that study.
We measured mRNA and protein expression of AQP4 and evaluated supramolecular aggregation in tissues of mice, rats, and humans. We studied tissues obtained at autopsy from control individuals and from 1 patient with NMO.
We evaluated tissues from 3 BL6 mice, 3 Lewis rats, and 2 human controls. Human tissue was obtained from autopsies performed at Mayo Clinic, Rochester, Minnesota, as approved by the Mayo Clinic institutional review board, with an interval from death to harvesting of approximately 24 hours; written informed consent was not required for analysis of autopsy samples. Tissues were flash frozen in liquid nitrogen and stored at −80°C. We also evaluated 5 additional human optic nerves from controls that were collected within 12 hours of death and stored at −80°C.
We analyzed mouse and human tissues using quantitative PCR. Total RNA was isolated from approximately 30 mg of pulverized tissue using an RNA purification kit (RNeasy; Qiagen). After purification, the RNA samples were stored at −80°C for future analysis. Mouse tissue was analyzed by using SYBR-Green chemical analysis with primers that were selective for M1 and M23 isoforms of AQP4 as described by Noell et al.13 Human tissue was analyzed with 2 assays: gene expression assay–based hybridization probes based on 5′ nuclease chemistry (TaqMan; ABI) and a SYBR-Green–based assay (Invitrogen). The following mouse AQP4 primers were used: forward primers for AQP4 M23 (460–base pair [bp] product), 5′GGAAGGCTAGGTTGGTGACTTC3′ and for AQP4 M1 (510-bp product), 5′CTCCCAGTGTACTGGAGCCCG3′; common reverse primer 5′TGGTGACTCCCAATCCTCCAAC3′; and the HPRT1 (250-bp product) primers 5′GCTGGTGAAAAGGACCTCT3′ and 5′CACAGGACTAGAACACCTGC3′. Human tissues were analyzed with primers that recognize the shared sequence of M1 and M23 isoforms (total AQP4) rather than with primers specific for individual isoforms. The short 15-bp segment of the unique 5′-untranslated sequence of M23 human AQP4 precluded successful design of an M23-specific primer for either of the quantitative PCR techniques. Human HPRT1 and AQP4 primers were designed by Applied Biosystems using TaqMan MGB (minor groove binder) probes for the TaqMan assays. Primers for the SYBR-Green assays were as follows: for AQP4, 5′GCACCAGGAAGATCAGCATCG3′ and 3′GATTTCCATGAACCATGGTGACTC5′ and for HPRT1 (94-bp product), 5′TGACACTGGCAAAACAATGCA3′ and 5′GGTCCTTTTCACCAGCAAGCT3′. Threshold cycle (Ct) values in each reaction were determined in quadruplicate; variations in readings were required to be less than 0.6 Ct. The comparative Ct method (2−ΔΔCt method) was used to determine the relative expression of AQP4 to HPRT1 transcripts.
Frozen tissues were pulverized and homogenized in native polyacrylamide gel sample buffer (Invitrogen) containing 10% n-dodecyl-β-D-maltoside to a final concentration of 0.75%. Lysates were centrifuged at 5000g for 10 minutes at 4°C. Supernatants were spun at 20 000g for 30 minutes at 4°C and frozen in aliquots at −80°C. Samples for denaturing electrophoresis were added to 2× Laemmli sodium dodecyl sulfate (SDS)–containing loading buffer, incubated at 4°C overnight, and loaded onto an SDS-containing 18% polyacrylamide gel (Bio-Rad) without preheating. Proteins isolated from HEK293 cells transfected separately with human M1 or M23 AQP4 isoform pcDNA, a commercial cloning vector (Invitrogen), were loaded as molecular weight controls. Loading of protein on gels was optimized to visualize bands corresponding to the M1 and M23 AQP4 isoforms and to distinguish between them; to control for variations in the amount of protein loaded, the intensities of the AQP4 and β-actin control bands were compared visually and by using densitometry for semiquantitative analysis. To evaluate the supramolecular aggregation of AQP4, blue native polyacrylamide gel electrophoresis (BN-PAGE), a nondenaturing electrophoresis technique established for this purpose, was used as previously described.14 Briefly, protein supernatant was mixed in a 1:3 ratio with sample buffer and G-250 (a detergent; Coomassie ratio, 4:1) and loaded onto a 3% to 12% Bis-Tris gel and electrophoresed with native PAGE running buffers, dark blue, and light blue according to the manufacturer's protocol (Invitrogen). Two-dimensional PAGE was performed according to the manufacturer's protocol (Invitrogen). Briefly, native PAGE gel strips were cut along loading lanes and treated with a lithium dodecyl sulfate sample buffer (NuPAGE; Invitrogen) in a reducing solution followed by an alkylating solution and finally a quenching solution to equilibrate the proteins before loading onto the second-dimension SDS gel.
Proteins were blotted onto polyvinylidene fluoride membranes (Bio-Rad) using a blot module (XCell; Invitrogen) according to the manufacturer's protocol. Membranes were blocked in Tris-buffered saline and Tween 20 (TBST) with 5% nonfat dry milk and incubated with a 1:1000 dilution of rabbit anti-AQP4 (Santa Cruz Biotechnology) primary antibody at 4°C overnight. Blots were then washed in TBST and incubated with goat anti-rabbit IgG horseradish peroxidase (Southern Biotech) secondary antibody, washed, and exposed to a chemiluminescent substrate (SuperSignal ECL; Thermo Scientific).
We evaluated tissues from 3 BL6 mice. The mean RNA expression values for the M1 and M23 isoforms were highest in optic nerve and spinal cord tissues followed by brainstem and brain tissues. The kidney, a non-CNS AQP4-expressing tissue, had much lower expression than the CNS tissues did, and the liver, as expected, had no detectable expression (Figure 1A).
We evaluated tissues from 2 human autopsies. The TaqMan assay yielded higher values for AQP4 transcripts than the SYBR-Green assay did, but ranked expression results were strongly concordant using both methods. Messenger RNA (mRNA) levels for total AQP4 were highest in optic nerve and spinal cord tissues followed by other CNS tissues; non-CNS tissues had much lower expression, and liver tissue showed the lowest expression (Figure 1B).
Immunoreactive AQP4 M1 and M23 protein per unit of tissue weight varied considerably between the tissues of all species, but the relative expression was consistent in the NMO-relevant tissues analyzed across species (Figure 2); AQP4 was most abundant relative to β-actin, the comparator protein, in optic nerve tissue from each species. Spinal cord tissue also showed high levels of expression but lower expression than the optic nerve. Expression was much lower in kidney tissue; however, kidney tissue could not be distinguished from liver tissue, which is established not to express AQP4.
Central nervous system tissue had adequate amounts of immunoreactive AQP4 for evaluation of supramolecular aggregation when we used BN-PAGE (Figure 3). Gels were loaded so as to adequately visualize higher-order bands, which were the bands of particular interest; this necessitated overloading of protein of low aggregate size. In all the species evaluated, the highest ratio of large to small AQP4 aggregates was found in optic nerve tissue, and the lowest ratio was found in tissue from the cerebral hemisphere. The relative abundance of highest-order aggregates to lowest-order aggregates was also evaluated by using 2-dimensional BN-PAGE, which confirmed an excess of higher-order aggregates in optic nerve tissue and showed that these aggregates had a high proportion of the M23 isoform, particularly in the optic nerve, as expected (Figure 4).
We compared total AQP4 mRNA levels in the optic nerve of a patient with NMO with those of 5 control optic nerves using the SYBR-Green method (Figure 5A). The NMO sample showed intermediate numbers of transcripts compared with the controls. The relative amounts of total AQP4 protein and the relative amount of M23 vs M1 expression as determined by SDS-PAGE did not differ between the control and NMO tissues, nor did the distribution of higher-order aggregates (Figure 5B-D).
Formation of OAPs is required for binding of NMO-IgG.15,16 Mutations that disrupt the formation of supramolecular aggregates and OAP formation abrogate NMO-IgG binding.16 Hydrophobic interactions of 7 amino acids downstream of methionine 23 in AQP4 M23, especially Ala25 andPhe26, are critical to supramolecular aggregation and OAP formation. These residues interact with Cys17 to Ser21 of AQP4 M1 to disrupt the formation of OAPs. The interaction of these M1 and M23 residues may represent nonspecific blocking effects that do not depend on interactions of specific residues.10 Supramolecular aggregates are generated after AQP4 tetramers are transported to the cell membrane.17 Extracellular proteins, including agrin and laminin, are also critical to orthogonal array formation,18 as are elements of the dystrophin cytoskeleton that anchor AQP4 in the membrane. Interference with the interaction of M1 and M23 AQP4 isoforms or of AQP4 molecules with their cytoskeletal or extracellular anchors may disrupt the formation of OAPs. Given the critical role of the relative expression of M1 and M23 isoforms to supramolecular aggregation and OAP formation, an analysis of AQP4 isoform expression and supramolecular aggregation is relevant to understanding the tissue specificity of NMO-IgG targeting.
Aquaporin 4 is highly expressed in the CNS and is also expressed in some non-CNS tissues, especially skeletal muscle, crypts of the gastric mucosa, renal medulla (distal renal collecting tubule), lung, and placenta.19,20 Aquaporin 4 is concentrated in the CNS at astrocyte end-feet as a result of anchoring by the end-foot–concentrated dystrophin cytoskeleton. Messenger RNA of the M1 protein isoform has been reported by others as the predominant AQP4 transcript detected in the human and rat CNS.21,22 However, M23 is the dominant protein isoform expressed in mammalian tissues. The relative proportion of M23 to M1 varies between tissues, the highest proportion of M23 being in CNS tissues.19,20,22 The reason for the apparent dissociation between RNA transcript and protein isoform ratios is not entirely clear and may involve transcriptional, translational, and posttranslational mechanisms. The transcriptional activity of the M23 isoform is greater than that of the M1 isoform in choline acetyltransferase assays of promoters of the 2 isoforms. The differences are exaggerated in glioblastoma vs kidney cell lines, possibly explaining the tendency for relatively greater M23 expression in the CNS.21 Translation of M23 has been described as "leaky" because the Kozak sequence of the native M1 isoform translation start sequence deviates from what is considered optimal.20,22 This deviation results in generation of M23 proteins from M1 as well as from M23 transcripts. Finally, the disproportionate representation of the shorter M23 isoform in large molecular aggregates may be attributable to differential posttranslational turnover of the 2 different isoforms because larger aggregates may represent a pool of AQP4 less prone to turnover; after exposure to NMO-IgG, M23 aggregates are not modulated from the surface of astrocytes as readily as M1 isoforms.23
Neuromyelitis optica clinically targets the CNS selectively; no renal abnormalities have been recognized despite expression of both isoforms in the kidney. Myositis is a rare occurrence.24 Although some patients with NMO develop pathologic changes outside the optic nerve and spinal cord, it is unusual for this to occur in early phases of NMO. Severe necrotizing pathologic features are characteristic of optic nerve and spinal cord lesions but less so of lesions in the area postrema, a common inaugural site of pathologic manifestation, perhaps owing to relative deficiency of the blood-brain barrier and high AQP4 expression. Area postrema lesions may be nonnecrotizing, sparing myelin and astrocytes.25
Saini et al12 studied differential tissue expression of M1 and M23 using real-time PCR methods and RACE to differentiate 2 apparently different molecular species of M23: a genomic-encoded form with an M23-specific untranslated region sequence and a second mRNA alternative splice variant. The authors concluded that M1 isoform mRNA was the predominant isoform expressed in the optic nerve and spinal cord and suggested that the proportion of M1 may be key to NMO pathophysiologic changes. The mechanism underlying the putative association of high levels of M1 mRNA in the optic nerve with pathogenesis of NMO were not explored, but the authors intimated that an epitope related to the M1 isoform might be important. The M1-specific isoform sequence encodes an intracellular domain of AQP4. Different from the rat and mouse M23-specific sequence, the human M23-specific sequence within the untranslated region of M23 is very short (15 bases) and AT rich (66.7%), restricting primer design; by contrast, the rat M23-specific sequence is much longer (105 bases).22 We quantitated the M1 and M23 isoforms of AQP4 transcripts by quantitative PCR in mouse tissue but were unable to do so in human tissue because of the short M23-unique transcript sequence. Both the M1 and M23 isoform transcripts were detected, and, in the mouse brain, the relative amounts of M1 and M23 transcripts paralleled the relative amounts of M1 and M23 protein. However, NMO is an autoantibody-mediated disease; therefore, protein expression is more relevant than RNA expression to the pathogenesis of the disease. In this study, we assessed M1 and M23 AQP4 protein expression in NMO-relevant tissues.
We report 2 key findings: (1) total AQP4 mRNA and protein per unit of tissue weight were greater in the optic nerve and spinal cord relative to other CNS and non-CNS tissues and (2) the distribution of supramolecular aggregate species of AQP4 differed, with the proportion of the largest aggregates highest in the optic nerve and, to a lesser extent, in the spinal cord. These observations may be relevant to why NMO selectively targets the optic nerve and spinal cord and spares non-CNS AQP4-expressing tissues, such as the kidney. The relative amounts of AQP4 and control proteins were evaluated in whole-tissue lysates, and the results were not skewed by the immunoprecipitation of AQP4 protein. Expression of total AQP4 mRNA and of protein products of both principal isoforms of AQP4 relative to control genes and proteins varied similarly in the 3 species evaluated. In CNS tissues, AQP4 was most highly expressed in the optic nerve and spinal cord at the mRNA and protein levels, which are the tissues most frequently and severely targeted in NMO. Furthermore, we found that the distribution of supramolecular aggregates of AQP4 was skewed toward those of the greatest molecular size in the optic nerve and spinal cord compared with other tissues. Aggregates of different sizes were observed in all the tissues studied, consistent with expression of both isoforms of AQP4 in all the CNS and extra-CNS tissues. We found no clear differences in mRNA or protein isoform expression between a single patient with NMO whose tissues we had an opportunity to study and multiple human control tissues; however, the fact that only a single NMO case was studied is a notable limitation of this study. Thus, extrapolating from this case, the key difference between patients with NMO and controls is the presence of NMO-IgG autoantibody in serum and not the molecular expression of AQP4, at least as measured with the techniques used in this study.
Our study did not distinguish white from gray matter in the spinal cord; immunostaining performed in other studies26 suggests that expression is higher in central gray matter than in white matter and that NMO lesions tend to be central in the spinal cord, although they involve both gray and white matter. However, the optic nerve, a white matter tract, had the highest expression of AQP4 per unit of tissue weight, and the differences between the tissues we studied are not explained by the relative proportion of gray vs white matter.
Some of the differences between CNS tissues and the differences between non-CNS tissues and CNS tissues could relate to the density of AQP4-expressing cells rather than to differences in the amount of AQP4 expressed per cell; comparators other than β-actin that are sensitive to astrocyte density may be of value in future studies. However, the differences between the optic nerve and spinal cord compared with the frontal lobe were large and unlikely to be explained by the density of astrocytes alone.
Other factors, such as differences in the inflammatory microenvironment or differences in proteins that mitigate complement-mediated pathologic changes, including complement inhibitory proteins (eg, CD59), and regional differences in blood-brain barrier characteristics could also contribute to differential pathologic changes in the CNS or in certain structures, such as the optic nerve and spinal cord. Further studies of the expression and aggregation of AQP4 may provide additional insights into the relative susceptibility of tissues to NMO pathologic changes.
Accepted for Publication: March 4, 2013.
Corresponding Author: Brian G. Weinshenker, MD, FRCPC, Department of Neurology, Mayo Clinic, 200 First St SW, Rochester, MN 55905 (email@example.com).
Published Online: July 15, 2013. doi:10.1001/jamaneurol.2013.3124.
Author Contributions:Study concept and design: Weinshenker.
Acquisition of data: Matiello, Schaefer-Klein, Sun.
Analysis and interpretation of data: All authors.
Drafting of the manuscript: Weinshenker.
Critical revision of the manuscript for important intellectual content: Matiello, Schaefer-Klein, Sun.
Obtained funding: Weinshenker.
Administrative, technical, and material support: All authors.
Study supervision: Weinshenker.
Conflict of Interest Disclosures: Dr Weinshenker reports serving on data safety monitoring boards for Novartis, Biogen Idec, and Mitsubishi; receiving payment for consultation from Elan Pharmaceuticals, Asahi Kasei Kuraray Medical Co, Ltd, GlaxoSmithKline Pharmaceuticals, and Ono Pharmaceuticals; serving on the editorial boards of the Canadian Journal of Neurological Sciences, the Turkish Journal of Neurology, and the Multiple Sclerosis Journal; receiving research support from the Guthy-Jackson Charitable Foundation; and receiving license royalties from RSR Ltd for a patent regarding AQP4-associated antibodies for diagnosis of NMO.
Funding/Support: This work was supported by grants from the Guthy-Jackson Charitable Foundation (Dr Weinshenker) and by postdoctoral fellowship grants from the National Multiple Sclerosis Society (Dr Matiello).
Additional Contributions: Michael Fautsch, PhD (Department of Ophthalmology, Mayo Clinic, Rochester), provided archived human optic nerve samples. Vanda Lennon, MD, PhD, and James Fryer, MS, provided stable cell lines expressing M1 and M23 human isoforms for use as molecular weight markers.


text:
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Notice to Readers: Better Hearing and Speech Month --- May 2008
Hearing loss affects one to three of 1,000 live-born infants annually (1,2). Without intervention at an early age, hearing loss can delay speech, language, social skills, and academic achievement. Therefore, all infants should be screened for hearing loss by age 1 month but preferably before leaving the birth hospital. All states and territories offer hearing screening for newborns. Any infant who does not pass the hearing screening should have a full hearing evaluation by age 3 months. If hearing loss is confirmed, the child should be referred for needed medical tests and begin intervention services by age 6 months (3). Following this 1-, 3-, 6-month plan for these children can maximize communication and language development (4,5). Information on CDC's Early Hearing Detection and Intervention programs is available at http://www.cdc.gov/ncbddd/ehdi.
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Date last reviewed: 5/1/2008


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Work has halted at one of the key facilities in the country's nuclear weapons complex following an act of civil disobedience July 28 by three Catholics, including an 82-year-old religious sister.
The Y-12 National Security Complex, the nation's only facility for manufacturing, processing, and storing weapons-grade uranium, has temporarily stopped operations and has moved all its nuclear material into secure vaults following the action, which saw the three Catholics cut through a series of fences before making their way to the complex's reportedly most secure facility.
The three, who call themselves the Transform Now Plowshares, said they wished with their action to "indict the U.S. government" for its nuclear weapons modernization program and for planning to build a new facility at the Y-12 site in Oak Ridge, Tenn., according to friends of the activists.
In the last several years, activists in the area have been protesting the planned new facility for the complex, called the Uranium Processing Facility, which the National Nuclear Security Administration says is needed to replace aging buildings and facilities, some of which date to 1945.
Federal estimates have placed the price tag of the new facility, one of three planned by the security administration, at about $6.5 billion.
The National Nuclear Security Administration (NNSA) released a statement Wednesday saying the temporary closure of the nuclear site was "necessary to ensure continued confidence in safe and secure operations at Y-12."
"During the stand down period, which is effective today and is expected to end by next week, all special nuclear materials will be moved to vault-type facilities on site, all nuclear operations will be halted, and contractor security personnel will undergo training and refresher instruction," the statement continues.
According to reports in the Knoxville News Sentinel, which first covered the protest, the activists were found outside the facility July 28 after they had hung banners, splashed blood and painted messages on the wall of the storage site.
Before they were arrested, the activists offered to share bread with the guards at the site and sang, the News Sentinel reported.
The three activists -- Sr. Megan Rice, a member of the Sisters of the Holy Child Jesus; Michael Walli, a member of the Dorothy Day Catholic Worker community in Washington, D.C.; and Greg Boertje-Obed, a former U.S. Army officer from Duluth, Minn. -- had their second court appearance regarding their protest Thursday.
They are expected to appear again Friday afternoon for a detention hearing in the U.S. District Court of Eastern Tennessee in Knoxville. At that hearing Magistrate Judge C. Clifford Shirley is to determine whether they can continue to be held in prison pending trial or if they can be released on bail.
For more on the protest, see NCR's earlier story, Catholic activists breach Tennessee nuclear weapons plant in protest.
Friends of the activists are also maintaining a website to keep people informed of their condition: Transform Now Plowshares.
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text:
Jazzfunk & Fusion pt. II (December 2012)
- by The Jazz Kid
- 2,837
- 53m
- 4 years ago
Swiss Jazz Quintet, Festival Big Band, Ian Carr, Nucleus, Scott Bradford and more.
+December 2012 list
+Jazzfunk & Fusion pt. II
+Selected and mixed by Blast Kid the Jazz Kid
+Recorded from original vinyl
+Record cover art and more lists on http://thejazzkid.tumblr.com
Since I discovered your mixcloud profile, I can't stop listening to your selections. And I always listen each mix at least twice. Every track is wonderful. Thanks for the good work.
yee very good make i come from jazz and funks yeeeeeeeee
beautiful selection, enjoyed every minute of it!
chillin.. out )
Gotta press the fav, no choice, FUNKtastic! Thanks
Scott Bradford?! Wow, haven't heard that for a while, thanks
ps - will share this on my advents calendar on the blog tomorrow, hope thats ok!
YAY! Been waiting for the December mix! Hope all is well with you my friend! Looking dope as always!
Thanks Dee. That Scott Bradford was recorded in Belgium by the way. The saxophone player is the one and only Nathan Davis and the rest of the band are the Belgian usual suspects...
Holy smoke.......that's some mean Rhodes on Safari, and what a killertrack by Dieter Reith!!! Never heard of Scott Bradford...but he surely swings. FANTASTIC! :=
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text: About Time Warner CableTime Warner Cable Inc. (NYSE: TWC) is among the largest providers of video, high-speed data and voice services in the United States, connecting more than 15 million customers to entertainment, information and each other. Time Warner Cable Business Class offers data, video and voice services to businesses of all sizes, cell tower backhaul services to wireless carriers and, through its NaviSite subsidiary, managed and outsourced information technology solutions and cloud services. Time Warner Cable Media, the advertising arm of Time Warner Cable, offers national, regional and local companies innovative advertising solutions. More information about the services of Time Warner Cable is available at www.timewarnercable.com, www.twcbc.com, www.navisite.com, and www.twcmedia.com. About Ipsos MediaCT (U.S.) Ipsos MediaCT is the market research specialization within Ipsos built to reach, engage and more effectively understand today's digitally-driven consumer in the fast moving media, content and technology space. We work with leading companies in technology, entertainment and all sectors of media – TV, online, print, mobile, outdoor, radio – helping owners and advertisers to better understand different audiences, the content they consume, the channels they use to consume it and the technology they use to discover, talk about and access this content. Ipsos MediaCT is a specialist division within Ipsos, one of the world's largest market research agencies. Ipsos has offices in 84 countries, generating revenues of €1,363 billion (1.897 billion USD) in 2011. Alongside media, content & technology, Ipsos has specialist practices in advertising, loyalty, marketing and public opinion research. Visit www.ipsos-na.com/media to learn more.


text:
Braden McGrath wrote:
>>Otherwise, you may look at a packet filtering software that
>>"properly handles stateful masquerading."
> To clarify though... M0n0 handles stateful masquerading just fine. It
> just doesn't handle some forms of stateful FTP masquerading, because to
> do so requires the firewall to basically rewrite FTP control packets as
> they come through. It's an added load to the CPU, and it adds to the
> size of the firewall, both of which are enemies on m0n0.
> As others mentioned, with OpenBSD's pf, you get an "ftp-proxy" program
> that handles rewriting... on the CLIENT side. It does this to make
> *active* FTP work with less hassle on the client side.
> I don't know how it would function if you tried to pass server-bound
> traffic through it though.
ftp-proxy is not designed for proxying FTP to servers, so it wouldn't apply.
pf however does work fine with the packet mangling involved in rewriting
the FTP traffic when sending control/data connections to a translated
address, including AFAIK port-translated addresses (port forwarding as
opposed to 1:1); hence why my FTP server on a private address can
recieve passive connections from the Internet just fine. (And thus no
need for ftp-proxy to have to try to work the other way around.)
Working around firewall "unfriendly" protocols such as FTP, IRC's DCC,
and other brainless apps are one sign of a well designed *modern* packet
filter. Even PIX manages to provide fixups.


text:
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text:
Gift Premium Subscription To ColColt
Join Date:04-21-2010
Last Activity:06-24-2012 7:18 PM
- Long Time Gun Enthusiast
- Shooting and Photography
Posted in thread: .357/.38 Spcl. on 06-10-2012 at 02:26 PM
For shooting 38 Specials primarily, this is my all time favorite. Accurate as you'll likely
find and locks up tight as Ft. Knox. groups have been excellent with a 158 gr cast bullet with
4-4.5 gr of 2...
Posted in thread: Moonclips on 11-21-2011 at 12:01 AM
Mine is the 357. Moon clips will work with rimmed cartridges also.Welcome to TK Custom.com &
Posted in thread: Moonclips on 11-13-2011 at 11:44 PM
Has anyone ever had a cylinder cut to accept full moon clips? I was considering this for my
SP-101 from TKCustoms.
Posted in thread: Another S&W on 11-13-2011 at 02:40 PM
My ex-wife was terrified of guns-any kind. She wouldn't even come in the same room if I had one
out. She said she was afraid they would accidentally "shoot off"!!:D Of course, she was
paranoid anyway....
Posted in thread: Another S&W on 11-13-2011 at 01:21 AM
Oh me either. And if I do ever get married it will be to someone that I don't have to negotiate
my hobby purchases with:DThe beauty of a good dog is...1)They like a lot of stuff on the
floor2)The don'...
Posted in thread: Another S&W on 11-13-2011 at 01:00 AM
You can always tell the wife you had lust in your heart and the devil made you do it.:D I don't
have a wife-just my dog so, that makes it easy for me.
Posted in thread: Another S&W on 11-13-2011 at 12:52 AM
I was going to buy the Colt Commander XSE but fooled around procrastinating and I lost it to
someone else. However, this one caught my eye and I decided to get it as an alternative. I like
the all blu...
Posted in thread: Commander XSE or 1911SC? on 11-05-2011 at 03:24 PM
I wouldn't cull either design as both have proven themselves. I've had less trouble with
external extractors(S&W specifically) than with others such as Colt and SA...my experience
only. If a pistol co...
Posted in thread: Commander XSE or 1911SC? on 11-04-2011 at 05:02 PM
Cane-The external extractor has been proven(to me at least) to be a very reliable method. I
have a 20 year old 4506 with the external extractor and a 6906-both 3rd Generation pistols and
neither at an...
Posted in thread: Commander XSE or 1911SC? on 11-03-2011 at 12:07 AM
MIM parts don't really bother me that much but I would like to see them not use it for the sear
and disconnector. Perhaps those parts are better than in days gone by but as you said, old
thoughts and ...


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text:
Calendar of Events
Spring 2014
March 13-14, 2014
Transmodernism: Intergrating the Art and Science of Movement
Transmodernism: Intergrating the Art and Science of Movement
A SymposiumThursday, March 13, 5:00-9:00 pm, Merrill Hall 131
Friday, March 14, 9:00 am - 3:00 pm, Merrill Hall 131
Cosponsored with UWM's Dance Department in the Peck School of the Arts and the College of Health Sciences
FREE for UWM students and ACDFA attendees
$35 one day | $50 both days
Tickets at the door and online through Brown Paper Tickets: http://www.brownpapertickets.com/event/577227
All proceeds to the Ed Burgess Legacy Scholarship Fund
The way we all interact with our environment is through movement.
With an increasing dependence on technological advances, our movements have become more automated and routine, which leads to both physical and psychological stress. As habitual movers, we rarely think about how we move, and when we do we often attempt to direct ourselves as if an outside observer was providing a posture for us to imitate.
Somatic methods, such as the Alexander Technique, are one approach to affecting one's balance of intention and attention during movement within the environment. The Alexander Technique has been successfully used with a wide range of performers from elite dancers to patients with severe neurological problems or pain. This symposium brings together dance instructors, physical therapists, and Alexander Technique teachers and practitioners to discuss and experience the relations between attention and intention to action from a variety of perspectives.
Glenna Batson (PT, ScD, AT Instructor, Professor Emeritus, Duke University) is keynote speaker.
Thursday, March 13
Arrival 4:45-5:00 pm
Welcome and Intro talk 5-5:15 pm
James Robey – Transmodernism in Dance 5:15-6:15 pm
Joint Lecture about AT / PT 6:15-7:00 pm
Exploration work / Glenna Batson 7:00-8:00 pm
Panel discussion 8:00-9:00 pm
Friday, March 14
Welcome and recap intro for those who are new to the symposium 9:00-9:30 am
Lessons / one on one work / small groups demonstrations 9:45-11:45 am
Lunch and science lecture 12 noon-1:00 pm
Panel on PT, Dance and AT interactions 1:00-2:00 pm
Keynote: Glenna Batson 2:00-3:00 pm
The Transmodernism symposium is based on the work Luc Vanier (Dance) and Wendy Huddleston (Kinesiology) did in their C21 Transdisciplinary Challenge Award project: Intention and Attention: Transmodernism and Integration in Human Movement Studies.
Transmodernism symposium website
Transmodernism flyer (PDF)
< Return to Calendar
- postal address: p.o. box 413 milwaukee, wi 53201
- street address: curtin hall 929 3243 n downer ave milwaukee, wi 53211
- phone: 414.229.4141
- fax: 414.229.5964
- email: firstname.lastname@example.com


text:
Offensive Outburst Takes Ports Past Giants 10-1
By Stockton Ports | June 23, 2013 1:40 PM ET
STOCKTON, Calif. - The Stockton Ports offense had struggled mightily over the first three games of their series with the San Jose Giants. That all changed on Sunday afternoon at Banner Island Ballpark. Stockton hammered out 13 hits that yielded 10 runs in a 10-1 win over the Giants as the Ports salvaged the final game of the four-game set.
San Jose scored their only run of the game in the top of the first. With runners at the corners and nobody out, Angel Villalona grounded out to third to bring in a run and give San Jose a 1-0 advantage. The run was unearned and aided by an errant pick-off throw from Ports starter Raul Alcantara (1-0), who allowed just the one run in his California League debut. Alcantara went on to earn the win as he tossed five total innings and allowed four hits while walking two.
Stockton's offense took hold of the contest in the bottom of the first. With two on and one out, Giants starter Clayton Blackburn (3-4) gave up an RBI single to Tony Thompson that tied the game at 1-1. B.A. Vollmuth followed with an RBI double to left to put the Ports in front to stay. With two on and two out, Rashun Dixon hit a ground ball to shortstop and Bobby Haney's throw went into the dirt and into foul ground, an error that allowed two additional runs to score and give the Ports a 4-1 advantage.
The Ports widened their lead in the second on an RBI single hit by Addison Russell to make it a 5-1 game.
Blackburn pitched into the fourth inning and allowed back-to-back singles with one out to Myrio Richard and Bobby Crocker that put runners at the corners. Russell came up and hit a sac-fly to right to score Richard from third. Max Muncy followed with his league-leading 19th home run of the season, a two-run shot that made it an 8-1 ballgame and knocked Blackburn from the contest.
Blackburn suffered the loss after going 3.2 innings and allowing eight runs (six earned) on eight hits while striking out three.
Seth Frankoff took over for Stockton in the sixth and pitched around a pair of singles and a hit batsman to keep San Jose out of the run column over two innings worked. Tucker Healy entered the game in the eighth and tossed two scoreless frames to close out the contest.
After Jason Forjet pitched 3.1 scoreless innings for the Giants, Kelvin Marte came on to pitch the ninth and, with one on and two out, gave up a two-run homer to Russell that increased the Ports lead to 10-1. It was Russell's eighth home run of the season and it made him 2-for-3 in the contest with four RBI.
Richard went 4-for-4 on the afternoon with three runs scored. Seven of the nine Ports batters got a hit during the contest.
The Ports welcome the Visalia Rawhide to Banner Island Ballpark to begin a three-game series on Monday. Josh Bowman (1-1, 11.91 ERA) will take the mound in search of his second consecutive victory for Stockton, opposed by Visalia left-hander Keith Hessler (5-3, 6.57 ERA). First pitch is set for 7:05 p.m. PDT.
This story was not subject to the approval of the National Association of Professional Baseball Leagues or its clubs.View More


text:
It's not in cinemas until May 2005, but bosses at Lucasfilm behind the Star Wars saga decided to tell fans now.
Episode III is the film in the saga that links the two recently made films to the original three made years ago.
The film will show how Darth Vader becomes the most feared baddie in the Star Wars universe and how loads of Jedi get wiped out in the Clone Wars.
Fans are expecting the first trailer to be shown around the time the original trilogy are released on DVD - in September 2004.
The film's title was revealed at a huge event in the US called Comic-Con in San Diego.
Loads of cool stuff about top films is announced at the event every year.


text:
If Hudbay Minerals Inc. takes over Augusta Resource Corp., it will inherit far more than a massive copper mine site outside Tucson, a potential for huge production and profits, and an equally massive controversy.
It will face a truckload of legal obligations and commitments to mitigate and compensate for the mine's environmental impacts. It will also face questions and concerns from the community about how real those commitments are — questions that don't always have simple answers.
Over the past seven years, Rosemont Copper and its Canadian parent Augusta have promised verbally and in writing to carry out dozens if not hundreds of mitigation measures for the planned Rosemont Mine in the Santa Rita Mountains. Those commitments have mushroomed in number and scale as the mine has inched closer to final federal permitting decisions, which Augusta expects by June but which Hudbay has predicted will take much longer.
Toronto-based Hudbay is nearing the final stages of its Augusta takeover effort. On Friday, it extended the deadline for Augusta shareholders from Wednesday to April 2 to decide whether to accept the takeover bid. Augusta's board of directors has recommended rejecting what it calls Hudbay's "grossly inadequate" and "opportunistic" offer and says it's already negotiating with other potential suitors.
If either Hudbay or another company buy Augusta, will Rosemont Copper's promise to pay for a 7-mile-long Central Arizona Project pipeline hold up? Among other examples, what about its plan to use dry-stack mine tailings, which use less water than traditional tailings; or its agreement with residents to compensate them if their wells go dry from mine pumping?
Hudbay has promised in emails to the Star and to the Sahuarita Sun to continue Rosemont's commitments.
The U.S. Forest Service says it will have a staff hired at company expense to make sure that 100-plus pages worth of mitigation measures it required of Rosemont Copper are carried out on the ground, regardless of who owns it.
One Forest Service staffer would work full-time coordinating the monitoring and enforcement of the requirements. A monitoring team representing other federal agencies will evaluate whether the mine's impacts live up to what was predicted by the final Rosemont environmental impact statement, said Coronado National Forest Supervisor Jim Upchurch.
The Forest Service, the Arizona Game and Fish Department, the Arizona Department of Environmental Quality and the town of Sahuarita also have various kinds of "successor clauses" in permits and agreements involving the mine, aiming to bind future companies to measures imposed on Augusta and Rosemont Copper. Such clauses are in general legally binding and enforceable in court by the agencies, although it may not be easy for average citizens to take such cases to court if they're not parties to the agreements, says William Sjostrom, a University of Arizona law professor who specializes in contract law and the field of mergers and acquisitions.
"Measures that are a condition of permits are requirements of the project and would not be affected by a change in ownership," Hudbay spokesman Scott Brubacher wrote in a recent email to the Star. "We value the relationships we have with the people who live around our mines, we engage and we listen. Hudbay wants to play its part in strong, sustainable communities. Should the Rosemont project advance under Hudbay, that is what can be expected from us."
The project's opponents are skeptical that these commitments will hold up, even as they also say these measures are inadequate.
For one, some of the mitigation measures have come as verbal commitments only, with no formal, contractual arrangements to carry them out. Second, they've seen other mining companies promise to protect the environment in the past. A Washington, D.C.-based Rosemont opposition group, Earthworks, commissioned a study showing that 70 percent of mines they looked at failed to meet such promises when it comes to protecting water quality, even in cases where there weren't corporate takeovers.
Third, the opponents point to past cases in which other takeover companies have failed to follow predecessors' commitments to mitigation measures.
"Our view is that mitigation is never assured till it's actually done," observed Pima County Administrator Chuck Huckelberry, a longtime outspoken Rosemont opponent.
"Until you get the party that is going to do it agree to it and put it into a contract, there will always be questions and potential loopholes. There will be a misunderstanding," Huckelberry said. "Companies get sold again and go bankrupt."
Pipeline commitment
On Rosemont, the mitigation promise that has drawn the most attention in light of the takeover efforts is the proposed $25 million pipeline that would take CAP water south to the Green Valley-Sahuarita area to be unloaded into basins to recharge the aquifer. That recharge is supposed to compensate for Rosemont Copper's pumping of groundwater nearby to service the mine, lying over the Santa Ritas along Arizona 83.
Rosemont and Augusta have promised to build this pipeline in meetings, news releases and media interviews. Augusta has signed a letter of intent with Community Water Co. to build the line. It has paid $2 million to cover all costs incurred to date for permitting, right-of-way acquisition, design, engineering and a small amount of construction work.
And, it has bought and recharged 45,000 acre-feet of CAP water in Marana which it hopes to trade with some other entity for the rights to other CAP water that eventually could be shipped through the main project canal to the new pipeline for recharge near Green Valley.
Virgil Davis, the Community Water Co.'s board chairman, says the water company has a contract with Augusta in which the mining company commits to pay for the pipeline. But he said the water company doesn't want to release the contract publicly because of the criticism it expects would be voiced by mine opponents. Augusta has not put money in escrow to cover future construction costs.
If a takeover should occur, however, Davis said he has no doubts that a successor company would build the pipeline, which the water company dubs Project Renews.
"The commitment and integrity of the Augusta people has been excellent," Davis wrote in an email to the Star. "Community Water and Augusta negotiated in good faith to develop an implementation plan for Project Renews that accommodated possible changes in the ownership of either party. That being said, we are committed to seeing Project Renews through to completion."
Among those who question whether the pipeline will survive a takeover are Tucson City Councilmen Kozachik and Richard Fimbres, utility watchdog Magruder, Rosemont opposition group Save the Scenic Santa Ritas, and Peter Davis, a Green Valley resident who recently filed an objection to Community Water's application to the state for a permit to build the recharge basins.
"In the event this takeover does occur, what assurance do affected parties such as (Community Water's) ratepayers or owners have that Hudbay will fulfill Augusta's commitments?" Davis wrote the state last month. "To date, I have seen none."
In a recent email to the Sahuarita Sun, Hudbay Vice President David Clarry said the company is familiar with permitting projects in various jurisdictions and that what is most needed is "respect for the process and stakeholders."
Clarry said that Hudbay intends to fulfill Augusta's promises to use dry stack tailings at the mine and to honor Augusta's agreement with well owners in neighboring Sahuarita Heights to compensate them if their wells are drawn down by Rosemont's pumping.
But UA law professor Sjostrom said that while he can't say for sure without seeing the relevant documents involving Rosemont mitigation, he believes that emails such as Clarry's probably aren't legally binding enough by themselves to stand up in court. He added, however, that the company wouldn't have to sign a formal agreement to make it enforceable.
"In other words, emails can be enough," he said, although they generally need to be supported by some form of consideration, which he explained as "'I promise to do something in exchange for your promise to do something and vice versa.'"
When the Star asked Hudbay if it would be willing to put Clarry's comments into a legally enforceable form, company spokesman Brubacher said, "A change of ownership would not change the requirements of any permits issued or any contracts signed. Discussion of new binding agreements regarding Rosemont prior to (Hudbay) becoming owners is premature."


text:
|You are in: Business
Wednesday, 29 January, 2003, 18:24 GMT
UK could face £11bn budget hole
The UK government may have to raise taxes by up to £11bn a year from 2005 onwards, an independent economic think tank has warned.
Chancellor Gordon Brown can take short-term solace from the fact that the Institute for Fiscal Studies (IFS) believes he will more or less meet his borrowing targets during the next two financial years.
According to the IFS, this will bring the government into conflict with its "golden" budget rule - which states that it is ok to borrow to invest, but that any additional debt must be repaid over the economic cycle.
If the Chancellor wants to stick to his spending promises on public services like health and education, he will have to increase taxes.
Income tax rise of 4p?
Just to break even he will have to raise his tax take by at least £4bn, and possibly by up to £11bn to have a safety margin, calculate the institute's tax experts.
That is equivalent to a tax rise of between 1p and almost 4p on the basic rate of income tax .
"There is a danger of the Chancellor missing the golden rule as we go forward", said IFS director Robert Chote when he presented the institute's closely watched Green Budget, which analyses the government's fiscal options.
And if the economy grows slower than currently forecast by the Treasury, the budget shortfall could be even higher.
Gordon Brown is expected to give the details of his budget plans for 2003-04 in March.
No war impact
Military action in Iraq should not have a major impact on Mr Brown's budget calculations, the IFS experts say.
The Chancellor has already set aside £1bn to cover the costs, while a sharp economic slowdown might trigger a revenue shortfall of a further £2bn.
The figures, though, are dwarfed in a budget with tax revenues of about £400bn and spending at a similar scale.
But what will hit the budget numbers, say the IFS experts, is a dramatic shortfall in corporation tax, well below the Treasury's forecast.
Government debt
Last November, in his annual pre-Budget report, Gordon Brown admitted that the days of government surpluses would soon be over.
After paying off billions of pounds of debt over the past five years, he admitted that he would run a budget deficit for the next two years.
But Mr Brown and his officials hope that a sharp recovery of the UK economy will bring a swift turnaround in the following years.
The IFS analysts are more sceptical.
They predict that government spending will rise faster than planned, while tax revenues will grow slower than the government predicts.
See also:
18 Dec 02 | Politics
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28 Nov 02 | Politics
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