text:
Dawn of the Rise of the Fallen Machines Now Available!
Hello Sunsetters (we just made that up, can you tell?),
We are excited to bring you big news today. First up, Dawn of the Rise of the Fallen Machines campaign add-on is now available! Yes, we know it's April Fools' Day. No, this is not a prank. You can purchase this by itself or get the Season Pass and get BOTH the Mystery of the Mooil Rig AND Dawn of the Rise of the Fallen Machines, as well as, the Weapons Pack at a discount.
Man vs. Machine: The savage battle that's been raging on for billions of years enters its latest bout in Dawn of the Rise of the Fallen Machines. A rescue mission to save Sam's old mentors from the Fizzco Robotics Factory quickly turns into an all-out assault to counter-hack and dismantle Fizzco defenses. Don't worry. For you, hacking equates to pulling triggers and laying waste to a bevy of upgraded Fizzco bots. Everyone has their strengths!
Featuring an all new story, weapons, traps, Amps, enemies, clothing, gameplay mechanics, and a massive new area to explore – Dawn of the Rise of the Fallen Machines is a techtonic addition to the Sunset Overdrive universe.
New Achievement
As an added bonus, we're celebrating April Fools' Day the only way Fizzie would – with the release of new achievements for ALL players. The "Worst Job in the Kingdom" achievement is unlocked by replaying and completing the "Floating Garbage" mission within par time (15 minutes) for a whopping 200 Gamerscore! There's also the "Fizzie Says April Fools!" achievement. Complete them, and see what happens. Let the pranks begin!
Sunset Overdrive is now $39.99**!
For those of you who haven't picked up Sunset yet, that is ok, we won't judge, but now is your perfect chance! Sunset Overdrive is now (and will be moving forward) only $39.99! Grab it today via your favorite retailer or download directly from Xbox Live. Be sure to grab the Season Pass too which includes all the additional content for only $19.99.
**$39.99 is only in effect in the US. If you're outside of the US prices may be different.
Content Update
With the new add-on comes some bug fixes and improvements. We want to let you know what is getting fixed. We apologize if any of these have prevented you from fully enjoying the game.
So here you go and we hope to see you all online foolin' around in Chaos Squad.
- Fixed a bug with Game DVR where the clip is recorded to the wrong profile after switching accounts via the Xbox Dashboard.
- Fixed a bug where a Game DVR clip would be recorded when the player has failed a challenge but reached a high score during the failed attempt.
- Fixed a bug where Game DVR would record a clip when Night Defense was successful even though the player did not reach a new high score.
- Fixed a bug where bombs from the Shield Buddy could potentially get trapped in a nearby player's shield.
- Fixed a bug where some UI elements would not appear in-game when multiple Xbox accounts are signed in.
- Fixed a bug where the poster credits sequence would not be visually displayed, but audio could be heard in the background.
- Fixed a bug where the piranha's in the "Suddenly Piranhas" amp could potentially fall through the world when they spawn in.
- Fixed a potential crash that could occur when quickly accessing the weapon wheel after respawning with a loading screen.
- Fixed a bug where the player could potentially have a blank trap screen and not be able to edit their trap loadout.
- Fixed a bug where the D-pad trap selection UI and various POI are missing from HUD when a secondary profile is signed in before launching.
- Fixed a bug where not enough Fizzco enemies would spawn during the "Awesomesmithing" mission if the Chance of Lightning amp kills enough of the Fizzco enemies.
- Fixed a bug where the failed screen would continue to appear after reloading the last checkpoint in the "Find 4Kim" mission.
- Fixed a bug where the failed screen would continue to appear after reloading the last checkpoint if the player fails to keep up with the train in the "Help a Hobo" quest.
- Fixed a bug in the "Excalamune" challenge where it will not end if the Spawner is killed before the Herker.
- Fixed a bug in the "Episode 4 – Buck Stops Here" challenge where the player would be taken to their primary mission location instead of the actual challenge location.
- Fixed a typo in one of the intro images for "Fizzco Ambush" mission in Chaos Squad.
- Fixed a bug where some Chaos Squad missions could potentially be assigned as active in the Campaign.
- Fixed a bug where a client would be unable to pick-up the battery after being killed by the battery in the "Power Up" mission in Chaos Squad.
- Fixed a bug where the 4th and 5th spinners for the Chaos Squad rewards would no longer appear once the player has earned all the amps and vanity rewards.
- Fixed a bug in Chaos Squad where the player could potentially lose their weapon loadout during the "The Floor is Lava".
- Fixed a bug where the last outfit purchased with Calista could still be displayed as purchasable.
- Fixed a potential bug where vanity items owned could be shown as available to buy with Calista if an Insomniac Set was purchased but not equipped.
- Fixed a bug where the Dev Shirt could potentially disappear from the players inventory.


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Boys and girls benefit from literacy programme
International research has consistently found that girls outperform boys in reading and writing - but one Scottish education authority is bucking the trend with its literacy programme.
North Lanarkshire's active literacy initiative shows significant improvements for boys and girls, according to senior educational psychologist Nancy Ferguson.
Analysis found that in both the control group and the active literacy group, girls outperformed boys at the beginning of P1. But six years into the programme, the gender gap had disappeared.
"Both did equally well," Dr Ferguson said. "In the control groups, however, girls continued to do better than boys and this difference was statistically significant."
Dr Ferguson and members of the authority's literacy base believe that certain features of the active literacy programme have engaged boys more than traditional methods: its active thinking and challenging approach; more opportunities for discussion, investigation and problem solving in a structured way; no time-wasting; and more opportunities for extended periods of reading and writing.
Its multisensory approach and the physical manipulation of magnetic letters and boards at the early stages seem to suit boys.
An underlying philosophy is the removal of decontextualised workbooks and worksheets, which means that pupils engage in fewer low-level tasks and spend more time on high-level tasks. Instead, there is a list of approved children's books, graded for different stages, from which teachers work.
Teachers have often observed that boys who started school before the age of five were more likely to struggle throughout their education. But when North Lanarkshire spent five years tracking children who had started school at the age of four, they found they recorded an average standardised score of 112 - in the "above average range" of 111-131 under the Neale Analysis of Reading Ability (Nara) test.
Of the overall results for active literacy, Dr Ferguson said: "In the control groups where children didn't have active literacy, girls outperformed boys from baseline to P7, but with the active literacy group, this year's results show consistently that active literacy boys outperform boys who have not had this approach. And boys and girls perform at the same level in the active literacy group."
P5 Nara comprehension (Standardised scores)
Below average range (70-89)
Active literacy group 3.8%
Control group 22.9%
P5 Nara comprehension (Standardised scores)
Average range (90-110)
Active literacy group 35.4%
Control group 47.9%
P5 Nara comprehension (Standardised scores)
Above average range (111-131)
Active literacy group 60.8%
Control group 29.2%.

subset: commoncrawl


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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Retroviruses exploit nuclear trafficking machinery at several distinct stages in their replication cycles. In this review, we will focus primarily on nucleocytoplasmic trafficking events that occur after the completion of reverse transcription and proviral integration. First, we will discuss nuclear export of unspliced viral RNA transcripts, which serves two essential roles: as the mRNA template for the translation of viral structural proteins and as the genome for encapsidation into virions. These full-length viral RNAs must overcome the cell's quality control measures to leave the nucleus by co-opting host factors or encoding viral proteins to mediate nuclear export of unspliced viral RNAs. Next, we will summarize the most recent findings on the mechanisms of Gag nuclear trafficking and discuss potential roles for nuclear localization of Gag proteins in retrovirus replication.
Retroviruses interact with nuclear trafficking machinery during several different phases of their replication cycles (
Once integration is completed, viral RNA is synthesized by the cellular RNA polymerase II. Retroviral transcripts are co-transcriptionally modified with the addition of a 5' cap and 3' poly(A) tail, like cellular mRNAs. A fraction of the viral RNA is spliced and exported out of the nucleus to serve as mRNA for translation into viral proteins. The remainder of the viral RNA remains unspliced and must be transported from the nucleus into the cytoplasm where it serves two functions: (i) as the template for translation of the viral structural proteins, Gag and Gag-Pol; and (ii) as the viral genome, which is packaged into virus particles. Because export of unspliced and incompletely spliced cellular RNAs is prevented by host machinery to prevent translation of abnormal proteins (reviewed in [
The Gag protein, which directs the assembly and budding of virus particles from the plasma membrane, is localized primarily in the cytoplasm of infected cells. However, the Gag proteins of Rous sarcoma virus (RSV), feline immunodeficiency virus (FIV), mouse mammary tumor virus (MMTV), prototype foamy virus (PFV), murine leukemia virus (MLV), Mason-Pfizer monkey virus (MPMV), HIV-1, and several retrotransposons undergo nuclear localization under certain conditions (
Model of retrovirus replication. Retroviral infection is initiated with binding of the viral Env protein to a cell surface receptor and fusion of the viral envelope with the cellular membrane (step 1). The viral RNA genome is reverse transcribed from RNA to DNA (step 2) to form the provirus, which is stably integrated into the genome of the host cell (step 3). Viral RNA is transcribed by the host polymerase II, and a portion of the RNA is spliced to direct the translation of the Env glycoprotein and other viral proteins (step 4). A portion of the viral RNA remains unspliced and is exported from the nucleus by the host factor TAP/NXF1 (e.g., simple retroviruses MPMV and RSV) or virally encoded Rev-like proteins (e.g., complex retroviruses like HIV-1 and HTLV-I) (step 5) to serve as a template for Gag and Gag-Pol translation (step 6). The Gag proteins of some retroviruses traffic through the nucleus during assembly (step 7). It has been postulated that the nuclear population of Gag (denoted by "?") might select genomic RNA (gRNA) and transport it into the cytoplasm for packaging. Alternatively, selection of genomic RNA may occur in the cytoplasm (step 8). In either case, the Gag-gRNA complex is transported to the plasma membrane (step 9) where additional Gag molecules bind the viral RNP to complete assembly of the immature virus particle, which buds from the plasma membrane (step 10). The steps in replication that are covered in this review are indicated in bold. Figure modified from [
Retroviral proteins that undergo nuclear trafficking*
|Retroviral Protein
|Localization of the Population Associated with the Nuclear Compartment
|Nuclear Localization Mechanism
|Nuclear Export Mechanism
|RSV Gag
|Nucleus [
|Imp11 (MA domain) [
|Nucleoli [
|Importin α/β [
|RSV RT, β subunit
|Nucleus [
|Nucleus [
|Shares Import Pathway with Histone H1 [
|MMTV Gag
|Nucleoli [
|Unknown [
|No Identified NES
|Nucleoli [
|MMTV Rem
|Nucleoli [
|Retrotranslocation from Endoplasmic Reticulum to Nucleus [
|MPMV Gag
|Nuclear Pore Complex; Low Levels in Nucleus [
|Unknown; Nuclear localization Increased with Ubc9 Overexpression [
|JSRV Rej
|Nucleus and Nucleoli [
|Rej Function Depends on CRM1 [
|MLV Gag
|Nucleus [
|No Identified NES
|Nucleus [
|MLV p12
|Mitotic Chromatin [
|Nucleus and Nucleoli [
|Unknown; Interacts withBrd4 in Nucleus [
|HTLV Rex
|Nucleus and Nucleoli [
|Importin β [
|BLV Rex
|Punctate Nuclear Foci [
|HIV-1 Gag
|Nucleolar [
|Not CRM1 [
|Nucleus [
|Importin α3 [
|Nucleoli [
|HIV-1 Rev
|Nucleus andNnucleoli [
|Importin β [
|HIV-1 Vpr
|Nucleus [
|Importin α [
|HIV-2 Rev
|Nucleoli [
|HIV-2 Vpx
|Nucleoplasm [
|No Identified NES
|FIV Gag
|Nucleus [
|FIV Rev
|Nucleolus [
|EAIV Rev
|Nucleus [
|BIV Rev
|Nucleus and Nucleoli [
|Importin α [
|MVV Rev
|Nucleoli [
|CAEV Rev
|Nucleoli [
|PFV Gag
|Nucleus [
|Binds to H2A/H2B on Mitotic Chromatin [
* Note: Transcriptional activators related to HIV-1 Tat were not included in the table.
Productive retroviral infection requires unspliced viral transcripts to be transported into the cytoplasm where they are translated into the essential viral proteins Gag and Gag-Pol. To circumvent intrinsic cellular blockades that prevent the export of incompletely spliced RNAs from the nucleus, complex retroviruses encode
HIV-1 Rev recognizes and binds to the highly structured
In the nucleoplasm, Rev co-localizes with the SR-domain splicing factor SC-35 in nuclear speckles [
HIV-1 Rev mediates export of unspliced viral RNAs through an interaction between the NES of Rev and the CRM1/RanGTP export complex. However, many other cellular proteins also interact with Rev, indicating that nuclear export of unspliced HIV-1 RNA depends upon a complex network of interactions [
In contrast to complex retroviruses that encode
Insight into the mechanism by which CTE-containing RNAs are exported from the nucleus was provided by proteomic studies that identified the host nuclear export protein Tip-associating protein/Nuclear RNA export factor 1 (TAP/NXF1) as a binding partner of CTE complexes [
A putative
RSV RNAs containing the DR elements are exported by the cellular mRNA export factor TAP/NXF1 and the RNA helicase Dbp5 [
Taking the available data into account, we postulate that there is a temporal switch in RSV replication, such that viral transcripts produced early after integration are exported using DR-mediated interactions with Tap/NXF1 and Dbp5 to initiate the synthesis of Gag and GagPol proteins. We hypothesize that as the levels of these viral structural proteins increase, the Gag protein enters the nucleus where it may bind unspliced viral RNA and export it into the cytoplasm for encapsidation into virions [
FVs, members of the genus
Among retroviruses, PFV utilizes a unique set of cellular factors that bind to its viral RNA for nucleocytoplasmic transport. Rather than using TAP/NXF1, like the
Historically, the Gag proteins of orthoretroviruses were thought to exist only in the cytoplasm of infected cells. However, it has recently been shown that Gag proteins of diverse retroviruses localize to the nucleus under specific conditions [
Localization of Gag proteins in and near the nucleus. The Gag proteins of retroviruses and retrotranspons have been detected in the nucleoplasm (MLV, PFV and RSV), in association with chromatin (PFV), in the nucleolus (FIV, HIV-1, MMTV, and RSV), at the nuclear rim (MPMV, Ty1, FIV) and at pericentrosomal sites (HIV-1 and PFV).
The first retroviral Gag protein discovered to undergo active nucleocytoplasmic trafficking was RSV Gag, which has a well-understood mechanism of nuclear entry and egress. Whereas under steady-state conditions RSV Gag is detected primarily in the cytoplasm and along the plasma membrane, treatment of RSV-infected or Gag-expressing cells with the CRM1-inhibitor leptomycin B (LMB) dramatically concentrates Gag in the nucleus [
To dissect the mechanism by which RSV Gag undergoes transient nuclear trafficking, a series of experiments were performed to identify nuclear import and nuclear export signals (NES) in the RSV Gag polyprotein [
The MA domain does appear to be a critical determinant of RSV Gag function, as replacement of the RSV MA sequence with HIV-1 MA abrogates nuclear trafficking of the RSV/HIV chimera [
Why does RSV Gag contain two NLSs that interact with three different import factors? One possibility is that Gag encodes redundant signals to ensure it enters the nucleus. Alternatively, each NLS might be used selectively: the NLS that mediates entry of the Gag precursor during assembly could use one or more sets of import factors whereas the MA and NC NLSs in the mature proteins could interact with different importins during early infection. In support of this idea, insertion of a canonical NLS into MA (in addition to the atypical NLS already present) interferes with infectivity [
Although the mechanism of nuclear trafficking is the best understood for RSV Gag, other orthoretroviral Gag proteins also localize to the nucleus or at the nuclear rim (
Subcellular sites of Gag-genomic RNA interaction where genomic RNA packaging may be initiated prior to plasma membrane localization. Retroviral Gag-RNA complexes have been visualized in pericentrosomal locations (HIV-1 and PFV) [
The lentiviral FIV Gag protein has a substantial degree of nuclear localization under steady-state conditions in feline cells, its natural host [
In the same study, HIV-1 genomic RNA also accumulated at the nuclear envelope [
Like FIV, a link may exist between MPMV Gag accumulation at the nuclear pore complex (NPC) and genomic RNA packaging (
The yeast long terminal repeat (LTR) retrotransposons Ty1 and Ty3 have some trafficking properties in common with retroviruses. Ty1 and Ty3 encode Gag proteins that bind unspliced genomic RNA to form virus-like particles. Mex67p, the yeast ortholog of TAP/NXF1, is required for the export of Ty1 RNA from the nucleus [
The Gag proteins of RSV and HIV-1 localize to the nucleolus under certain conditions [
Due to the transient nature of its trafficking through the nucleus, detecting RSV Gag in nucleoli requires disruption of the nuclear export activity of Gag, either by mutating the p10 NES or treating infected cells with LMB [
Other retroviral Gag proteins have been reported to localize to the nucleolus under certain conditions. For example, LMB treatment of transfected cells causes FIV Gag to accumulate in nucleoli when expressed alone and nucleolar localization was dependent on NC [
In addition to localizing to the nucleolus, the RSV Gag protein also accumulates in discrete nucleoplasmic foci when restricted to the nucleus by mutating the NES or inhibiting CRM1-mediated nuclear export [
In the past decade, great strides have been made in understanding the mechanisms that guide nuclear import, nuclear export, and the intranuclear activities of retroviral and retrotransposon Gag proteins. The use of a comparative approach to identify common and unique features of different Gag proteins in the nucleus has been very illuminating. Although it is not yet clear whether all Gag proteins transit through the nucleus at some low level, examination of the roles of nucleocytoplasmic trafficking in those Gag proteins that do enter the nucleus or associate with the nuclear envelope (RSV, MPMV, MLV, HIV-1, FIV, PFV, Ty1 and Tf1) are important to pursue.
Is Gag nuclear trafficking involved in genomic RNA packaging? To date, studies of RSV, MPMV, and Ty1 support this idea. PFV Gag associates with chromatin and plays a role in integration, although it might also function in genome encapsidation of nuclear transcripts [
Finding that several retroviral Gag proteins undergo nucleolar localization raises intriguing questions about whether there is a unifying function of the nucleolus in virus replication. One possibility is that retroviral Gag proteins interact with a host protein or RNA in the nucleolus involved in genome packaging or virus assembly. It is intriguing that several RNA pol III transcripts are enriched in retroviral particles, including RNAs that are processed in the nucleolus such as tRNA, U6 small nuclear RNA, 7SL [
This work was supported by grants from the National Institutes of Health R01CA76534 (LJP), P50GM103297 (LJP), F30 CA165774 (DVB), F31 CA171862 (RJK) and CURE Funding from the Pennsylvania Department of Health (LJP). The Pennsylvania Department of Health specifically disclaims responsibility for any analyses, interpretations, or conclusions of this project.
The authors declare no conflict of interest.


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Get an overview of this hotelHotel with free breakfast, near St. Augustine Beach Park
Popular property highlights
Situated near the beach, this hotel is within 3 mi (5 km) of St. Johns County Ocean Pier, St. Augustine Beach Park, and Anastasia State Park. St. Augustine Alligator Farm Zoological Park and St. Augustine Lighthouse and Museum are also within 6 mi (10 km).
Hotel Features
This hotel features an outdoor pool, coffee/tea in a common area, and laundry facilities. Free continental breakfast, free WiFi in public areas, and free self parking are also provided. Other amenities include a 24-hour front desk, express check-out, and a front-desk safe.
Room Amenities
All 38 rooms provide conveniences like refrigerators and microwaves, plus free WiFi and flat-screen TVs with cable channels. Guests will also find coffee makers, free local calls, and hair dryers.Information missing or incorrect? Tell us!Opens in a new window
St. Augustine Island Inn
Hotel Amenities
Hotel Amenities
A complimentary breakfast is offered. Wireless Internet access is complimentary. This St. Augustine hotel also offers an outdoor pool, coffee/tea in a common area, and laundry facilities. Limited complimentary onsite parking is available on a first-come, first-served basis.
St. Augustine Island Inn has designated areas for smoking.
- Number of buildings/towers - 1
- Outdoor pool
- Free breakfast
- Safe-deposit box at front desk
- Total number of rooms - 38
- 24-hour front desk
- Express check-out
- Laundry facilities
- Number of floors - 3
- Elevator/lift
- Designated smoking areas
- Parking (limited spaces)
- Free self parking
- Coffee/tea in common areas
- Free WiFi
Family Friendly Amenities
- Family Hotel
- Outdoor pool
- Free Wi-Fi
- Laundry facilities
- Refrigerator
- Microwave
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking, Parking (limited spaces)
Room Amenities
- Free WiFi
- Connecting/adjoining rooms available
- Air conditioning
- Free local calls
- Refrigerator
- Coffee/tea maker
- Microwave
- Daily housekeeping
- Hair dryer
- Iron/ironing board
- Cable TV service
- Flat-panel TV
- In-room climate control (air conditioning)
- Rollaway/extra beds (surcharge)
Where to Eat
Guests are offered a complimentary continental breakfast.
Nearby Things to Do
Recreational amenities at the hotel include an outdoor pool.
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
Hotel Policies
Check-in time ends at midnight
Check-in time starts at 3 PM
Check-out time is 11 AM
Payment types
Children and extra beds
- Children are welcome.
- Rollaway/extra beds are available.
- Cribs (infant beds) are not available.
- Pets not allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No rollaway/extra beds available
No cribs (infant beds) available
Optional extras
- Rollaway beds are available for an additional fee
Hotel Name
- Island Inn St. Augustine
- St. Augustine Island
- St. Augustine Island Inn
- St Augustine Island Saint
- St Augustine Island Hotel Saint Augustine
We should mention
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.


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First Image Of Russell Crowe As Noah
A sneak peek at Aronofsky's latest
The first image of Russell Crowe playing the title role of Darren Aronofsky's 2014 bibical epic, Noah, has arrived online via USA Today and you can check it out below.
For a bigger version of this image, head to USAToday.com
As eagle-eyed readers will notice, there's not a lot much going on in the picture apart from Crowe's rugged face, but there's still something rather encouraging about this extra piece of proof that the Iceland-shot project is genuinely happening.
Written by John Logan (Gladiator, The Aviator, Hugo and many more) and Aronofsky himself, it's not a traditional retelling of the age-old bible story but a radical reimagining, complete with giant angels called 'Watchers'.
As for the rest of the cast, you can look forward to the likes of Jennifer Connelly (playing Joan of Ark Noah's wife, Naameh), Douglas Booth and Logan Lerman (Noah's sons, Shem and Ham), Emma Watson (a close friend of Shem's, Ila), Anthony Hopkins (Noah's grandfather Methuselah, who is as old as, well... he's very old) and Ray Winstone as an as-yet unnamed character who acts as Noah's enemy.
If you want other hints and titbits about the project - including pictures of puffins - be sure to follow Aronofsky on Twitter where he's posting regular photos of the production as it happens.
Noah is set to hit cinemas in the UK and the US on March 28, 2014.


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Belila recipe And Books
|Super Recipe Contest
Belila recipe and books
So you would like to know how to make Belila recipe! It is not hard, follow the steps below and enjoy your own creation. Check our books section as well!
225g whole wheat grains soaked overnight in water
pinch of salt
425ml milk
1 tbspn caster sugar
2 tbspns aromatic honey
4 tbspns rose water
115g flaked almonds
50g pine nuts
seeds of 1 pomegranate (optional)
Rinse the wheat place in a pan cover with water add salt and bring to the boil.
Simmer gently halfcovered for 35 minutes.
Drain. 2
Bring the milk sugar and honey to the boil add the wheat and simmer for about 1ols minutes. Withdraw from the heat and mix in the rose water nuts and pomegranate seeds. 3
Offer with extra nuts hot milk and sugar.
Belila is an old Egyptian dessert which has recently become fashionable in smart Cairo hotels but it has changed its identity and it is now served as a healthy breakfast to the trend and health conscious. The dish consists of whole wheatgrains which have been boiled and are then served with sweetened milk nuts and flavourings such as orange flower water or rose water. The wheat expands in volume and should not be overcooked or it will turn into a mass. So keep an eye on it towards the end. Many variations of the dish can befound round the Mediterranean. For instance the Greek kolyva offered at funerals and on All Souls Day is similar but in a dry form without the milk and with a larger variety of nuts and spices. The same can be said about the Turkish asure which has an enormous variety of ingredients forty according to tradition and it includes rice and pulses such as beans and chickpeas as well as assorted driedfruits. This again is a kind of thick milkypudding and is supposed to be what Noah cooked with all the ingredients he had on the ark. All these dishes seem related to the ancient Greek panspermia a selection of wheat and nuts offered to the dead and the God of Hades.
This Recipe Serves 6
We truly hope you will like this American recipe, and enjoy your tasty Belila recepie. Check out these great recipe books and learn how to make Belila!
View Recipe Books - Recipe
Thank you for visiting Lovely Recipes and Books Web Site!
Website Design And Hosting - Abacus.ca


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Zimbabwe to Boston and Points in Between
The Boston Marathon is widely recognized as the premiere running event in the US and abroad. For 26.2 miles, approximately 20,000 runners push themselves to their limits while being cheered on by over a half million spectators. Among this year's finishers was Dr. Munashe Chigerwe.
Like many of the marathon runners, he traveled a great distance to Hopkinton , Massachusetts to be among the thousands to partake in the annual Patriot's Day celebrations and marathon kick-off. Originally from Zimbabwe , a slight detour brought Dr. Chigerwe to the College of Veterinary Medicine where he has been a food animal resident since 2003.
Many train for years to build their endurance and strength to even qualify for the marathon. Growing up on a farm and miles away from school, Dr. Chigerwe's training began at an early age. He fondly recalls running to school with his five siblings after morning chores to beat the bell.
Like many young boys in Zimbabwe , soccer was the main attraction with Dr. Chigerwe. While several of his siblings trained to be both short and long distance runners, he stuck with soccer through high school. Due to the lack of organized soccer here in the US , he decided to run in the time that he had.
Starting modestly with locally organized 5k runs, Munashe soon came to the conclusion that these short runs were not much of a challenge. A trip to Des Moines , Iowa in 2005 provided the first opportunity for him to test his long distance running. His summation of the marathon was, hard.
Undaunted, Dr. Chigerwe continued to train and participate in marathons when time permitted. On his fourth marathon, he qualified for the 2007 Boston Marathon. The 2007 race was characterized by bad weather including thirty-six mile per hour winds, rain and temperatures below 40 degrees.
The marathon itself as Dr. Chigerwe described was not only a physical, but mental roller coaster. Starting from corrals containing a thousand other runners, they began the first part of the race on relatively level terrain for the first twenty miles as they spaced out and found a comfortable pace. The remainder of the race is known for its increasingly challenging terrain of long rolling hills, including Heartbreak Hill, which depleted most of his energy reserves by the time he descended into Boston on adrenaline alone.
With one Boston Marathon under his belt, he has set his sights on another challenge. In a bid to qualify for the Marathon once again, he intends to run at least one marathon in every state before returning home to Zimbabwe . His current count is at eight with only forty-two more to go.
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Last Update: February 29, 2012


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Assembly pcr
From OpenWetWare
Assembly PCR
Assembly PCR can be used to assemble two pieces of DNA into one piece. Briefly, it essentially involves PCR'ing the two pieces separately with primers that have a 20bp overlap and then doing an extra PCR step using the two products as the template. This is essentially just for ease of cloning. Instead of trying to PCR two separate pieces and then assemble them by endonuclease digestion and ligation, it can be easier simply to PCR the first piece w/ a reverse primer that overlaps with the forward primer of the soon-to-be adjacent piece, and then use the product of the first PCR reactions as a template for the assembly reaction. If the overlap of the reverse primer for the 5' piece and the forward primer for the 3' piece overlap by ~20bp, the product of the first PCR reactions should anneal in the overlapping region and create a full length product. Using the Fw primer for the first piece and the Rev primer for the second piece in the assembly reaction then amplifies the desired full-length product.
Remember that this technique works only if: You want to assembly in series two pieces of DNA from PCR product (or you already have one piece you want to cut out and assemble in series w/ a PCR product; in this case it's easier just to do the PCR for the piece you already have. Believe me, the assembly reaction product is well worth the cost of the extra primers and PCR step).
- 1) Design the reverse primer for the DNA that will be 5' w/ significant overlap w/ the forward primer for the 3' piece. Essentially, as long as one of the primers has ~20bp overlap w/ the reverse complement of the other primer, the products should anneal in the assembly reaction.
- 2) Do PCR as normal for the 5' and the 3' pieces using the longer primers that correspond to each piece.
- 3) Check on a gel to make sure you got product from the first PCR reaction. Some people like to cut out the product band and use the purified products as template for the next reaction. I just use the PCR product straight from the first reactions w/o any purification; the logic is that the undesired primers/templates will be in such low concentrations that the intended reaction will be highly favored.
- 4) Set up the assembly reaction like a regular PCR, except: 1) as template use equal amounts of product from the first reactions, and 2)use the Forward primer for the 5' piece and the Reverse primer for the 3' piece to amplify the annealed template. (I use 45µl of Invitrogen Taq HIFI supermix, 2µl of 5µM primer each, and 0.5µl of each PCR product as template). Cycle like you did for the first reactions.
- 5) Run the product on a gel. If the reaction worked, you should see a band the size of the sum of your two templates.
- 6) Purify the product (I use the Quiagen PCR pur. kit), cut w/ desired endonucleases, and clone away! The quality of the product from this reaction is usually very good and I can get up to >100 transformants. Felix Moserfelix moser


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Brian Sutton
Associate Professor
- Office
- Phone
- (920) 465-2027
- email@example.com
Professor Sutton mainly teaches courses in composition, humanities, and occasionally literature.
Research Interests: His research interests include composition, especially research-based writing and literature, and he publishes in both of these areas.
Recent Publications: In May and June of 2013, Venture Theatre of De Pere staged two one-acts of Dr. Sutton's as a double bill for two weekends. They were "Running Mates" (comedy, not a musical) and "Can I Buy You a Drink?" (a music revue—that is, a series of songs with sketches between, as opposed to a "book musical" with a plot from beginning to end). The director, cast and crew received a favorable review in the Green Bay Press-Gazette, and aided by that review and favorable word of mouth, the final performance was a sellout!
In July 2013, Dr. Sutton's full-length musical (regular "book musical," not a music revue) Searching for Romeo had four performances in the Jean Weidner Theatre.
"We sold out all four performances, received standing ovations at two of them, and received a favorable review in the Green Bay Press-Gazette. On the Monday after the final performance, the house manager of the Weidner Center contacted me and asked that I try to bring the play back for another weekend, because a number of customers on leaving the theatre had expressed interest in seeing it again, and also because after the opening, a number of people had tried to buy tickets based on word of mouth and expressed disappointment that the remaining performances were already sold out. Unfortunately, I wasn't able to reassemble the cast, since a couple of key cast members moved away the week after the run."
"Prince Charming and Peter Pan in Munro's 'How I Met My Husband.'" Notes on Contemporary Literature 42.2 (March 2012): 4-6. Print.
Education: Ph.D., English, Ohio State, M.A., English & Education, University of Michigan , B.A., English, University of Michigan
- English 218: World Literature I
- Humanistic Studies 323 The Writings of the Old Testament
- Humanistic Studies 324 The Writings of the New Testament


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Linkin Park Showcase Upcoming 'Recharge' Video Game at Berlin Press Event
Linkin Park have their hands in many different pies and one of their latest projects finds them working on a new video game. Showcasing the interest in energy and natural resources, the band tailored the game, titled 'Recharge,' around those issues.
The group teamed with Kuuluu Interactive Entertainment to create the game, which is a third-person 3D online experience that combines puzzle, adventure and fighting elements. However, rather than competing with foes, you're working in unison with other players as part of a rebellion to regain natural resources from those who hold them in power.
Mike Shinoda stated at a recent press event in Berlin (seen in the video below), "The story of the game is basically rooted that in this future world, the earth's natural resources have almost run out, so as you can imagine with the way that people are to one another, the people who have control of the resources have the power and they're basically using it to keep everyone else as their subordinate. As a player you're a member of the rebellion against that and you work with your group to beat those guys, to take back the power and to free the human beings."
Shinoda expressed his satisfaction with the whole game creation process, explaining, "That's like a dream come true, you know, we got to work on these characters and this game. The band gave the input on the story. I think we gave the original story and then went back and forth with Kuuluu on it and these guys translated that into some really interesting gameplay."
It's the band's plan to also tie in charitable upgrades, with users having the opportunity to add special features for their character with the proceeds from sales going to help aid natural resource issues in the real world. For more information on the 'Recharge' game, click here.
Watch Mike Shinoda and Joe Hahn at the 'Linkin Park Recharge' Press Event
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Watch a Trailer for the 'Linkin Park Recharge' Game
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Charlie Sheen Just Gave Lindsay Lohan $100,000 For Her 'Tax Bill'
Seen here at the premiere of Liz & Dick that she showed up two hours late for and only her dad liked because that will make up for recording every single phone conversation they've ever had, Lindsay Lohan apparently had $100,000 of her $233,904 unpaid tax bill knocked out by Charlie Sheen which I'm sure has absolutely nothing in common with the time she stole $100,000 from another rich dude then blew him to duck the charges. The two stories barely sound alike. TMZ reports:
Don't ever say Charlie Sheen's not the charitable type — because he recently cut Lindsay Lohan a $100,000 check to cover the actress' six-figure tax bill … all out of the goodness of his heart … sources tell TMZ.
Sources close to Lindsay tell us she and Charlie became close pals while on the set of "Scary Movie 5″ back in September — spending a lot of time together both on and off the set.
During their bonding period, we're told Lindsay and Charlie talked about everything — and at one point, Lindsay mentioned her ongoing tax problems.
During the course of his life, Charlie Sheen has done exactly three things out of the "goodness of his heart," and I guarantee you not one of them is give a woman money that's not payment for sexual favors (Legend has it he'd burst into flames.) because here they all are in order:
1. Not "accidentally" shoot Denise Richards.
2. Not "accidentally" shoot Brooke Mueller.
3. Not "accidentally" shoot his own kids.
And let's be frank, not a single one of those are set in stone. Emilio Estevez gives the kids new bulletproof vests every year for Christmas. True story.
Photos: Fame/Flynet, Getty, INFdaily, Pacific Coast News, WENN


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When Charles M. Steiner retired as the CEO, founder, and chair of Branch Group Inc. several years ago, he was renowned as a leading entrepreneur in the electric industry; indeed, today Branch Group, as part of Rexel, is the largest electrical distributor in the world.
In 1973, 10 years after he graduated from the University of Pittsburgh with a Bachelor of Business Administration degree in accounting, Steiner purchased the first of his many businesses, GEE Electric Co., in Wheeling, W.Va. After he grew GEE Electric into a successful business, he founded IMARK, an electrical cooperative; EDIC, an insurance company; and Vantage Group, a national sales organization to serve the electrical supply needs of major companies. Steiner now manages the Steiner Family Office.
Steiner's impressive contributions to the electrical industry have been recognized by his service as director and chair of various committees of the National Association of Electrical Distributors (NAED), for which he was presented the NAED Distinguished Service Award.
Through the Charles M. Steiner Atrium, Charles and Rhoda Steiner have provided students and faculty of the Joseph M. Katz Graduate School of Business (KGSB) with a beautifully enriched facility. The Steiners also established the Steiner Family Foundation Scholarship in Basketball, and, most recently, have made a leadership gift to create The Chesapeake & Potomac Pitt Club Grant Program that will provide need-based assistance for deserving students from the metropolitan Washington, D.C., area to attend Pitt.
Charles Steiner serves on the Pitt Board of Trustees and the Board of Visitors for the College of Business Administration (CBA) and KGSB. In April 2001, he delivered the CBA commencement address, and, in 2003, he was named a KGSB Distinguished Alumnus. He also is a Pitt Alumni Association Golden Life member.
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Unisex Hooded Unitard
Style: T180
Microfiber Polyester/Spandex
Unisex hooded unitard has a built-in glove and zipper closure that runs from the top of the head down to the waist. The unique foot features a sturdy sewn-in suede front sole for optimum comfort, durability and gripping action. Unitard is designed to be worn with optional head covers for the dancer who wants to be covered from head to toe. Black body features a black suede sole, other colors natural suede sole.
Girls: 4-6, 6X-7, 8-10, 12-14 Size Chart
ADDITIONAL Full Body Unitard & Head Covers - Girls Styles:


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Here's a great confirmation of Frankincense essential oil's cancer-fighting abilities. In this study, Boswellia sacra essential oil was used, though in other research, Boswellia carteri and Boswellia seratta have demonstrated these same effects. (See more here on each of these Frankincense CO2 essential oils, and our new Sacred Frankincense).
This is one in a great many peer-reviewed scientific studies confirming the anti-cancer action of Frankincense, at concentrations which leave healthy cells unaffected. (Here's our review of research using Frankincense against breast cancer cells).
Additionally, it was the heaviest-molecular-weight constituents of the Boswellia sacra Frankincense essential oil that were shown to have the most potent anti-cancer action. Good to know, as the CO2 distillations of Frankincense carried by The Ananda Apothecary DO have naturally have greater amounts of these larger molecules than steam or hydro-distilled varieties.
As in previous studies, researchers bathed human cancerous cell cultures in solution containing Frankincense essential oil, which caused apoptosis – or 'natural cell death' – of these cells. (This does NOT usually occur with cancerous cells; instead they are 'immortal').
What's new about this study is that tumors of these same cancerous cells were implanted in hairless mice. This allowed very direct observation of tumor size.
The 'experimental' group was injected at the tumor site with a Frankincense essential oil & saline solution, and the 'control' group with the saline solution only. Significant reduction in tumor size(s) was noted at only 14 days!
Researchers concluded that Frankincense essential oil might be a "might be a useful alternative therapeutic agent for treating patients with pancreatic adenocarcinoma, an aggressive cancer with poor prognosis".
Truly, EVERY study we've read investigating Frankincense as a treatment for cancers has concluded in a similar manner – that Frankincense warrants further investigation as a cancer treatment.
For more on this subject, visit www.pubmed.gov and search for 'frankincense cancer' or 'essential oils cancer'. And here's a link to this particular research abstract, and to the full research article.
(By the way, we do not, in any way, condone animal testing! However, it is only through such studies that natural remedies will be accepted by the "Western" medical community. We do believe it would be a beneficial to the world if Frankincense essential oil was given as a valid option for cancer treatment).
Please note: this article does not constitute medical advice, nor have these statements been evaluated by the FDA. No claims are made as to any medicinal value of this oil. The information presented here is for educational purposes of traditional uses and is not intended to diagnose, treat, cure, or prevent any disease.


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by Staff Writers
Diyarbakir, Turkey (AFP) Feb 21, 2013
Turkey sent jets across its border with Iraq to strike separatists from the outlawed Kurdistan Workers' Party (PKK), a local military source said Thursday.
The jets bombed 12 targets in the Kandil Mountains in the autonomous Kurdistan region of Iraq on Wednesday, the source told AFP without providing any casualty figures.
Pro-Kurdish Firat News Agency confirmed the raid, saying it targeted two villages and destroyed many farms and orchards.
The raid comes amid nascent peace talks between Ankara and PKK leader Abdullah Ocalan, who is serving a life sentence in prison.
Turkey hopes the talks will yield a ceasefire and eventually bring an end to the almost three-decade Kurdish insurgency for self-rule in the southeast.
Last month, the Turkish army said it killed 14 rebels in the southeastern city of Hakkari as they were preparing to ambush a border post. A Turkish soldier was also killed in the ensuing shootout.
Ankara says the rebels use their hideouts in northern Iraq, where the PKK command is also located, to infiltrate Turkey for hit-and-run attacks on security forces.
Around 45,000 people are believed to have been killed in 29 years of fighting between Turkish security forces and the PKK, which took up arms in 1984.
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Secondary vehicle help
View Single Post
Registered User
Join Date: Sep 2008
Location: Chwk, BC
Posts: 842
My Ride: 2007 X5 3.0Si
View My Garage
Originally Posted by
Is there any way you can swing an 06' and up X5? Two of my neighbors have X5's, and their parking spaces are side by side. But the e70 just looks so much better compared to an e53, when you see them side by side. Even my wife, who only pays attention to cars when I make her look at one, has commented how much better the e70 looks. They just look so much more muscular. This particular e70 has that Tobaco Brown leather interior, which is awesome, and the e53 has a black interior, so that makes this particular e70 look even better. She has decided that the e70 x5 is the vehicle she wants when we move to the 'burbs.
The Merc looks too generic for me. It does not have the personality of the BMW. I realize that is not a very thoughtful analysis, but it would be hard for me to get past that when it came down to buying one.
I have always heard good things about the Acura MDX. I know a few people who have had several of them, including one co-worker who trades his in every 2 years for a new one. But, he always gets the same color combo, which I think is a bit odd. I think it looks very generic as well. but it is hard to criticize the quality and durability of an Acura. I have never heard about any transmission issues, and did not see more complaints about it compared to the BMW on a quick Google search. It is worth looking into though, since an '04 would be out of warranty.
The Acura and the Merc both tend to blend in to the SUV crowd. The BMW's seem to stand out a bit more, particularly the e70. Have you done your homework on things like resale value, insurance, and cost of ownership estimates? I imagine the cost of ownership on the X5 is a bit high, compared to the MDX. Insurance is probably not much different between the three of them, but you never know. One may be less than the others. I would be interested to see how the resale values stack up among these three though.
Great feedback,
I've checked resale and cost maintenance
Mdx definitely comes out on top in those categories
Just started looking into the 4runnerd a bit too
Mercedes and range are out of the running
Cost wise X5 might be out of the running, it will be the secondary be the anyways but ill still hold some faith for test drives
I'd just have to look more into 4runner vs MDX now as I've owned Hondas before but not toyotas
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Keon Raymond scored the first touchdown of his life off an interception at the age of nine. That set the tone for his football career.
The Calgary linebacker is one away from becoming the franchise leader in defensive touchdowns as the Stampeders (4-1) face the expansion Ottawa RedBlacks (1-4) for the first time Saturday at McMahon Stadium.
Raymond tied Frank Andruski's club record last weekend when he intercepted former teammate Kevin Glenn and went 63 yards for the TD in Calgary's 25-24 loss to the B.C. Lions.
"It would be a blessing if I was able to get another one," Raymond said Friday. "I don't worry about it. If the opportunity presents itself, I want to take full advantage of it."
The 31-year-old is in his seventh season wearing Calgary's galloping horse logo. Raymond signed a contract extension with the club in January.
"It was one of the things I said when I signed back here … 'I want to be able to have my name on the side of this stadium one day,'" Raymond said. "That's one of the things that continues to fuel me."
Raymond's pick-six against B.C. was his fourth. The other two TDs have come on fumble recoveries.
He dabbled on offence as a receiver and running back in high school, but said nothing beats the feeling of scoring off the opposing offence's mistakes.
"It sucks the life out of them, and I don't mind being a vampire," he said.
A staunch Calgary defence allowing a league-low 15.1 points per game has been a huge difference for Calgary this season. The club's offence is the least productive in the West Division, averaging 24.6 points per game.
However, B.C. became the first CFL team this season to surpass the 100-yard rushing plateau against Calgary.
"Our biggest thing is to make sure we're in position to win the game at all times," Raymond said. "We did that. We just didn't close out defensively, so we want to make sure we're able to bounce back and continue to play defence the way we've been playing."
A former Stampeder quarterback returns to McMahon a second consecutive week, although Ottawa's Henry Burris spent two seasons as Hamilton's starter following his seven-year stint in Calgary. However, Raymond's familiarity with Burris will certainly help him anticipate his one-time teammate's moves.
And in 2012, Raymond returned a Burris interception 100 yards for a touchdown, but he says he won't shirk his defensive responsibilities for a shot returning a second Burris pick for the team record.
"I played with Hank for about five years and understand how he throws, what his looks are and what his main targets are," Raymond said. "It gives me a little bit of a bead on him, but I still have to make sure I'm doing my part, covering guys and [being] in the right position."
Ottawa is coming off a 38-14 home loss to Saskatchewan and is averaging just 18.8 points a game, ahead of only the Montreal Alouettes (15 points per game).
However, Ottawa remains in contention in the East Division. The Toronto Argonauts are the only Eastern club with more than one win and are on their bye week.
"That's the silver lining behind all this," Burris said. "You never expected the East would get off to this slow of a start. We need to take full advantage of it. That's what we've been preparing ourselves for all week."
"Toronto has the bye this week, so we have the chance to tie them up. Hamilton is thinking the same thing as well as Montreal, so who is going to take that next step? It's sitting right there for us. We need to make that step happen and it's going to start with our offence."
The Stampeders supplied the RedBlacks with significant manpower, both on their roster and coaching ranks.
Ottawa selected Glenn, offensive lineman J'Michael Deane and safety Eric Fraser in the expansion draft. But Glenn asked for and was granted a trade to the Lions after the RedBlacks signed Burris as a free agent.
The Stampeders also sent veteran offensive lineman Jon Gott to Ottawa for the first overall pick in the CFL draft in May, using it to take Laval offensive lineman Pierre Lavertu.
RedBlacks head coach Rick Campbell spent the past two seasons as Calgary's defensive co-ordinator, while offensive co-ordinator Mike Gibson is a former Stampeders offensive line coach.
Ottawa's first trade in February was acquiring defensive lineman Justin Phillips from Calgary, but Phillips is currently on the six-game injured list with a broken leg.
There wasn't much for Ottawa to celebrate against Saskatchewan but the club's first TD at home was certainly memorable.
When backup quarterback Thomas Demarco punted the ball into the end zone, an on-side Dobson Collins raced downfield and jumped on it for the score.
John Hufnagel, Calgary's head coach and GM, pointed out Campbell's coaching roots are on special teams.
"He's always been known to have some tricks up his sleeve," Hufnagel said.
With Canadian running backs Jon Cornish and Matt Walter still out with concussion symptoms, ratio issues will keep veteran slotback Nik Lewis a healthy scratch for a second week.
Cornish, the CFL's most valuable player and leading rusher last season, hasn't played since the season opener.
Cornish and Walter were both able to get into the weight room this week. Cornish also attended team meetings "so they're both going in the right direction," Hufnagel said.Report Typo/Error


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Maintaining a bench grinder wheel
Lots of DIYers own a bench grinder. But not everyone knows which wheel to use for which metal or how to care for the wheels. So we consulted the experts and came up with these nuggets to keep you in the grind.
First, use aluminum oxide wheels for ferrous metals and silicon carbide for all the others (brass, copper, aluminum).
Next, dress the wheels regularly to remove clogged grains. Use either a "star wheel" dresser or a diamond dresser available through our affiliation with amazon.com. The diamond style (not shown) "retrues" the wheel a bit. But don't expect either tool to "resquare" your wheel—that kind of rig costs a bundle.
Theoretically you can keep using your wheels until they wear down to the label. But you should replace them when they're too small to use with the tool rest. Before you install a new wheel or reinstall a used one, always "ring-test" it to make sure there are no cracks (Photo 2). Tighten the wheel only enough to prevent it from slipping. Overtightening can crack the wheel.


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in Ca. and now i live in south korea for the next year or
love to travle. so far i have been to tokyo, iraq, canada,
about 30% of the us. this year i plan to go to guam, japan
again,and travle up and down korea. Im trying to see the world
i want some one to see it with me. there is a lot more to
about me... so ask away
I am fun, awsome, and out going
He really is outgoing, and a really nice guy, not to mention he's
really super funny, and he's super generous,such a selfless guy,
that's why i am so inlove with him,.. you can hangout and be
friends with him, Just try not to flirt,.. ;)
guys..... i dont know cause we dont talk about that stuff
-- or if you see that i looked at your profile... some times i dont know what to say... so i just look and not say anything... but if you like what you see... say hi or wink at me... and i will respond to you


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Lions CEO Tom Lewand plans to start making calls at 12:01 a.m. ET.
So what should he do?
PFTV takes up the cause of the Lions in the latest installment of "Fix My Franchise."
Lions CEO Tom Lewand plans to start making calls at 12:01 a.m. ET.
So what should he do?
PFTV takes up the cause of the Lions in the latest installment of "Fix My Franchise."
Really not that many years away.
Hopefully they have their future franchise QB. If so, do the following:
1) Keep coaching staff together for at least a couple more years. Let them install their offensive and defensive schemes and go from there.
2) Get Suh or McCoy in the draft and add a veteran on the outside like Vanden Bosch or Kampman (as was stated before). Already have some of the other positions taken care of, just doesn't appear to be the case when you can't get pressure. This would instantly fix that problem. And they're young on defense so they'll continue to develop.
3) Get a RB, free agency (like Taylor) or draft (like Best) or both.
4) Get a reliable second WR. There's some out there right now. If you can't single cover Calvin Johnson, then it's trouble for any defense (with the exception of very few teams).
5) Draft a good young OT in the draft. I personally don't think the Lions O-Line is as bad as people think. They should play better if they aren't playing from behind and if the young players develop to learn better blocking and get the ball out of the QB's hand quicker. But you still need to look at the future.
All this sounds like a lot. It is. That's why it's still a couple years away. But they have potential to fix a great portion now and then look forward to being good in the near future.
Ok, so here is my approach to fixing the Lions. It is going to take longer then just this year, but here is where they should start.
Vanden Bosch DE
Thomas Jones RB
Chester Taylor RB
Resign Foote LB
Cromartie CB for Mo Morris and a 5th
Ginn Jr. WR for Sims or Avril
1st Suh DT
2nd Pouncey G-C
3rd Best available CB Donovan Warren or Patrick Robinson
4th Myron Rolle S
2011 Draft take LT, RB, DE in whatever order makes the most sense according to draft board.
Peppers? No thanks… absolutely not. I'd rather have Vanden Bosch, who hustles on each play, than Peppers, who disappears for weeks.
Your first step in fixing the Lions should be updating the helmet and logo you display on the monitors in the back. They have changed unis twice since the one you have up.
Since this will most likely be a response to my post, I might as well get it out of the way.
"They use the logo from the last time the Lions were relevant." Ha Zing.
Check out http://www.lionsgab.com to keep up on the Lions this offseason.
Yeah, all they have to do is sign all the top free agents and have all the top draft prospects fall to them in each round and their all set! Thanks for input guys!
Shouldn't this segment have been called "possible 2010 offseason moves"?


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Product Information
Dogs will put up a fight to play with this Tuff Guy Lars Squeaker Mat with more noises for hours of entertainment!
Why We Love It:
With 11 noisemakers and at least 5 different sounds, these Tuff Guy Squeaker Mat Dog Toys are sure to keep pooches occupied and happy! Each Tuff Guy has his own story, meet Lars. From an early age it was apparent that Lars was unable to grow hair on his head. When confronted about this topic, his response was simply, "Hair doesn't grow on steel." Lars found his true calling in ice hockey, where he was feared as the dirtiest player in Europe at the ripe age of 12. After years of intense skating and fighting, Lars has since made a quiet living for himself on his rustic ranch in Montana. He is pursuing a new passion as a country singer and hopes to release his first single in May of 2012.
Sizing Information:
18" Length x 9" Width x 3" Depth
Important Sizing Note: The following measurements are the dimensions of the actual product, not of the pet they fit. After measuring your pet, make sure all measurements fit within the following dimensions (with a little room to spare in the chest and neck).One Size


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Michael Parsons, Ph.D.
Primary Location
Cleveland Clinic Main Campus
- Appointment:
- 216.636.5860
- Desk:
- 216.445.1637
- Fax:
- 216.444.4525
Additional Locations
Brain Tumor and Neuro-Oncology Center
Cleveland Clinic Main Campus
9500 Euclid Avenue
Cleveland, OH 44195
Driving Directions
- Appointment:
- 216.636.5860
- Desk:
- 216.445.1637
- Fax:
- 216.444.4525
Treatment & Services
- Cognitive/neuropsychological Evaluations
- Neuropsychological Evaluation
Specialty in Diseases & Conditions
- Alzheimer's and Dementia
- Brain Cancer (Brain Tumor)
- Encephalopathy
- Epilepsy
- Memory Loss
- Stroke
Is Cleveland Clinic Part of Your Insurance?
Review a list of accepted insurance plans for our Northeast Ohio locations or learn more about purchasing a contracted managed care plan.View All Plans
About Michael Parsons, Ph.D.
Michael W. Parsons, PhD, joined the Section of Neuropsychology at Cleveland Clinic in 2007 as an Adult Neuropsychologist. He provides neuropsychological evaluations for adults for a broad range of referral questions, and consults to services throughout the clinic and community. These evaluations assist in diagnosis and treatment of medical and psychological conditions that affect brain function.
His research involves the use of functional and structural brain imaging techniques to study processes of learning and memory in cognitively intact individuals and patient populations. Other research interests include the effects of brain and other cancers on cognitive function, as well as the effects of cancer treatment on cognition.
Dr. Parsons is a Diplomate of the American Board of Clinical Neuropsychology and the American Board of Professional Psychology.
Education & Professional Highlights
Education & Fellowships
Residency - Medical College of Wisconsin Clinics
Milwaukee, WI USA
Medical School - University of Texas at Austin
Doctor of Philosophy
Austin, TX USA
Internship - University of Florida College of Medicine
Clinical Psychology
Gainesville, FL USA
Undergraduate - University of Virginia
Charlottesville, VA USA
Specialty Interests
Brain tumor, Dementia and Alzheimer's Disease, Epilepsy, evaluation of cognitive changes associated with other neurologic conditions including head injury, Huntington Disease, ALS, memory and memory disorders, memory executive functioning, movement and movement disorders, MS, Multiple Sclerosis, neurodegenerative diseases, neuropsychological assessment, neuropsychology, traumatic brain injury and other neurologic conditions
Research & Publications
See publications for Michael Parsons, Ph.D..
(Disclaimer: This search is powered by PubMed, a service of the U.S. National Library of Medicine. PubMed is a third-party website with no affiliation with Cleveland Clinic.)
Industry Relationships
Cleveland Clinic physicians and scientists may collaborate with the pharmaceutical or medical device industries to help develop medical breakthroughs or provide medical expertise or education. Cleveland Clinic strives to make scientific advances that will benefit patient care and support outside relationships that promise public benefit. In order for the discoveries of Cleveland Clinic physicians' and scientists' laboratories and investigations to benefit the public, these discoveries must be commercialized in partnership with industry. As experts in their fields, Cleveland Clinic physicians and scientists are often sought after by industry to consult, provide expertise and education.
To assure professional and commercial integrity in such matters, Cleveland Clinic maintains a program that reviews these collaborations and, when appropriate, puts measures in place to minimize bias that may result from ties to industry. Cleveland Clinic publicly discloses the names of companies when (i) its physicians/scientists receive $5,000 or more per year (or, in rare cases, equity or stock options) for speaking and consulting, (ii) its physicians/scientists serve as a fiduciary, (iii) its physicians/scientists receive or have the right to receive royalties or (iv) its physicians/ scientists hold any equity interest for the physician's/scientist's role as inventor, discoverer, developer, founder or consultant.* In publicly disclosing this information, Cleveland Clinic tries to provide information as accurately as possible about its physicians' and scientists' connections with industry.
As of 7/28/2016, Dr. Parsons has reported no financial relationship with industry that is applicable to this listing. In general, patients should feel free to contact their doctor about any of the relationships and how the relationships are overseen by Cleveland Clinic. To learn more about Cleveland Clinic's policies on collaborations with industry and innovation management, go to our Integrity in Innovation page.
Public Health Service-Reportable Financial Conflicts of Interest. Cleveland Clinic scientists and physicians engage in basic, translational and clinical research activities, working to solve health problems, enhance patient care and improve quality of life for patients. Interactions with industry are essential to bringing the researchers' discoveries to the public, but can present the potential for conflicts of interest related to their research activities. Click here to view a listing of instances where Cleveland Clinic has identified a Public Health Service (PHS)-Reportable Financial Conflict of Interest and has put measures in place to ensure that, to the extent possible, the design, conduct and reporting of the research is free from bias.
* Cleveland Clinic physicians and scientists subscribe to the guidance presented in the PhRMA Code on Interactions with Healthcare Professionals and the AdvaMed Code of Ethics on Interactions with Health Care Professionals. As such, gifts of substantial value are generally prohibited.


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Freimoser:Fast yeast transformation protocol
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- DNA plasmid
- carrier DNA (salmon/herring sperm DNA in H2O, 2mg/ml stock, 500 µl aliquots, heat-inactivated at 95°C for 5-6 min and put on ice before use (has to be done once))
- 50% polyethylene glycol (PEG) solution (MW 3'350, filter-sterilized)
- 1 M lithium acetate (autoclaved)
- water
- cells scrapped from plate
- plate containing appropriate medium
- Incubator
- Centrifuge
- Sterile 1.5-ml microcentrifuge tubes
- Measure out 50 µl of carrier DNA into sterile 1.5-ml microcentrifuge tube (1).
- Add cells scrapped from plate.
- Add 1 - 2 µl of DNA plasmid.
Add 240 µl of 50% polyethylene glycol (PEG) solution.
Add 36 µl of 1 M lithium acetate.
- Mix solution by pipetting up and down several times.
Option 1: Incubate at 30°C for at least 30 mins.
Option 2: Incubate at room temperature for at least 30 mins.
- Heat shock
Option 1: Store at 45°C for 15 mins.
Option 2: Store at 42°C for 20 mins.
- Centrifuge at maximum speed for 1 min at room temperature, gently aspirate out the supernatant and discard it.
Add 100 µl of water.
Resuspend pellet by vortexing/by shaking vigorously.
Plate out suspension onto plate containing appropriate medium.


text:
I'm in, please check out my portfolio
You rule!
Hey Lung, I would love one! I'll send you my mail address.
Thanks xtortionist, I was asking because envato sent me the mail so I thought it's normal here to send those DMCA 's. But why is then envato sending those emails when both authors are from Europe?
It's not that much a problem for me but if a author copies, really copies (doesn't post just the same type of file) and get's approved, he'll think it's a good thing.
Anyway, thanks for the replies, the thread can be locked.
Btw. the files are business cards, so plenty of room for creativity, nothing like logo mock-ups.
You don't have much possibilities with logo mock-ups, I've tried too make them as different as possible and would add more styles if there were more printing techniques. Maybe you should have compare the files first.
So you're saying when a designer (I don't wanna name anybody here) makes a flyer that's 70-80% your idea and makes it more stylish, it's alright?
I have a problem with a file which I think is pretty much similar to one of my designs, it's not the same but obviously a copy. I've contacted the support, first they told me that they can't do much about it, just tell the designer that it is too similar and ask him to modify it. The second mail from the staff was about a DMCA take down notice and now should I send one or not.
I don't know if it's allowed to post links so that you guys can compare the files and tell me if I'm right or insane
Congrats Rafael! Keep up the good work
Thanks guys, I truly appreciate it!
Just wanted to announce that today I've achieved my first milestone, 100 Sales. Big thanks to all buyers for their trust & fellow designers for the motivation!


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Anthrax vs. Linkin Park – Cage Match
Trash-metal legends Anthrax defeated Staind yesterday, bringing their Cage Match record to 4-0. With one more victory, Anthrax's 'I'm Alive' will be inducted into the Loudwire Cage Match Hall of Fame.
In what will be Anthrax's final bout with 'I'm Alive,' the band will face their biggest challenge yet, squaring off with the gigantically popular Linkin Park. While it may seem like an odd pairing, both these songs put the metal to the pedal.
Linkin Park's 'Blackout' has been remixed by one-time Nine Inch Nails member Danny 'Renholdër' Lohner for the brand-new 'Underworld Awakening' soundtrack. The experimental remix begins with a long electronic sequence, but never fear Linkin Park fans, the song goes into full-on assault mode at the one-minute mark.
Can Anthrax make it into the Cage Match Hall of Fame with their fifth straight victory? Or will Linkin Park start a winning streak of their own? Listen to both tracks and vote for your favorite song below.
Listen to Anthrax, 'I'm Alive'
Listen to Linkin Park, 'Blackout' (Renholdër Remix)
Rules of Cage Match:
There are no rules … except this one: If a song remains the reigning champ for five straight cage matches, it is retired to the Loudwire Cage Match Hall of Fame. With so many great songs out there, we have to give other bands a chance!


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Music: Mostly indie/alternative. Few go-to's are Portugal the Man, Spoon, Modest Mouse, Jack White, Arctic Monkeys, Black Keys
TV Shows: Archer, Bob's Burgers, Game of Thrones. Love a good netflix binge every now n then. Also any food-related show/documentary
Food: anything edible?
2. A lime (juiced)
3. Onion (chopped)
4. Roma tomatoes (seeded and diced)
5. Cilantro (finely chopped)
6. A large bowl to mix, stir and serve in
Oh, shit, I did this wrong didn't I?
*whispers* taxes
*panting softly* pay the mortgage
*moans* make sure we turned off all the lights, our utility bill was fucking $300 this month


text:
1 – Easy ordering process
2 – High quality output
3 – Satisfied customers
Your business has a lot to offer… and your website should reflect this fact! Custom product design software turns your business into a flexible online service. It helps you to cut costs, save time, eliminate waste by producing on demand, and build stronger customer loyalty.
Connect with us today to gain access to everything you need — We're standing by to help you implement the customized modifications and technical website improvements to turn your printing business into an efficient online operation.
Regardless of the size or shape of the products ordered, our personalized Product Design Software will help you support your customers' desire to make a bigger impact on their markets. The speedy turnaround time will enhance your customer's loyalty, and our dedicated customer service will ensure your complete satisfaction.
Products Designer started its journey from 2008 in web based custom Product Configurator Software industry.
We developed rich web applications to make printing business easy. Complete solution for digital printers with website and Online custom product designer software to sell any type of personalized product online like T-shirt, Business Card, Promotional items, Team Jersey, Cap/Hats, Shoe, Phone case, Laptop skin, mugs, bags, buttons or your imagination


text:
Apply for Winter Break Trips here today!
- Date: December 31, 2016 – January 8th, 2017
- Location: Jinotega, Nicaragua
- Theme: Global Youth Empowerment
- Cost: $1,850/Student (All-Inclusive)
UK ASB volunteers traveling to Jinotega, Nicaragua will work with the non-profit organization Outreach360 (formerly Orphanage Outreach), whose primary mission is to "Release the Hero Within – to involve people to provide opportunities to orphaned, abandoned, and disadvantaged children in Nicaragua." Specifically, volunteers will participate in Outreach360's Teach Corps-English Education program, helping to teach children basic English language skills in Outreach360′s Learning Center. Volunteers will stay in local housing provided by Outreach360 while in Nicaragua and partake of local food which will be prepared and served by Outreach360 staff. Staff will provide opportunities for volunteers to learn more about the history and culture of Nicaragua, including issues affecting youth and education, and volunteers will spend one free day as a group exploring the local area. This may include vising a local art co-operative, visiting a working coffee farm, walking trails through a cloud forest, and/or dining at an eco-resort. Group flights to Nicaragua will be booked by UK ASB, and students will depart as a group to/from Lexington. For more information about Outreach360, visit: www.outreach360.org.
- Date: December 31st, 2016 – January 8th, 2017
- Location: Amazon, Ecuador
- Theme: Environmentalism
- Cost: $1,850/Student (All-Inclusive)


text:
I'm pretty comfortable in the kitchen and would really enjoy cooking a nice, healthy meal for you. I love animals and if you have a pet that's a plus! I enjoy talking about current events. I'm fine if you have different opinions and enjoy a nice banter as long as we don't get too intense. One of my passions is learning about the new trends in technology - I guess I'm a bit of a geek when it comes to that kind of stuff.
For physical activities I'm open to just about anything. I do like being outside and so any type of running, hiking, or biking sounds great. I also like working out at my gym, rock-climbing and some snowboarding as well. I'm not really into the dance/bar scene any more, but would actually prefer getting to know you by going for a nice long walk or sitting at home watching a good movie and sharing a glass of wine.
i could go on and on
the internets
blue skys
good movies
haha and my awesome smile
I think a lot about what my next adventure is going to be.
I enjoy thinking about how my life could change over the next few years.
haha and i do day dream a lot of the day


text:
Barshilia, Harish C and Rajam, KS and Sudhakar, A and Sankar, N (2001) Design and development of a fully automated substrate rotation system (dwell time controller) for the deposition of multilayer coatings. Technical Report. National Aerospace Laboratories, National Aerospace Laboratories.Full text not available from this repository.
In the deposition of multilayer coatings (say of A and B materials) thicknesses of individual layers are of the order of few angstroms. The thicknesses of the individual layers are determined by dwell time of substrate underneath each target. Typically the dwell time is of the order of few seconds depending upon the process conditions. Manual control of accurate dwell time is very difficult. More so, the repeatability of the individual layer thicknesses greatly affects properties of the multilayer coatings. In order to deposit multilayer coatings with controlled layer thicknesses and repeatability a PC based control system is designed and tested. This system consists of a driver circuit power supply and a stepper motor connected to the substrate holder through a rotary feedthrough. A user-friendly software is written in Visual Basic and Visual C++ for driving the stepper motor. Keywords: Multilayer Coatings, Magnetron Sputtering, Dwell Time Controller, Visual Basic and Visual C++.
|Item Type:
|Proj.Doc/Technical Report (Technical Report)
|PHYSICS > Physics(General)
|Surface Engineering Division, Surface Engineering Division, Materials Science Division, Aerospace Electronics and Controls Division
|Depositing User:
|Mr N Selvakumar
|Date Deposited:
|24 Aug 2011 14:32
|Last Modified:
|24 Aug 2011 14:32
Actions (login required)
|View Item


text:
Palomar Mountain State Park appears to be saved from imminent closure, so hit those trails and celebrate in some of the most beautiful mountain scenery in our county.
Myers Fire Road trail
Before you go: Download a map of all Palomar Mountain State Park trails, including Myers Fire Road.
The map of the fire road shows a spur trail off the right near the end of that road; that's where the hairpin turn goes right to the cabin ruins.
Cost: $8 day-use fee.
Rules: Trails are open only to hikers (and leashed dogs only on Myers Fire Road).
Trailhead: To Myers Fire Road. From Interstate 15, take Highway 76 east. Follow signs to Palomar, going left on South Grade Road, S-6. When you reach the top of the mountain, follow signs to Highway S-7 to Palomar Mountain State Park. Once in the state park, park at Doane Pond day-use area. Walk to the campground and the beginning of Myers Fire Road near campsite No. 9.
Distance: Myers Fire Road is about 1.9 miles one way with an elevation gain of about 700 feet.
Difficulty: Moderate.
The 1,862-acre state park averages 5,000 feet in elevation with the highest point, High Point, rising to 6,140 feet, just east of the world-famous Palomar Observatory. Some 40 inches of rainfall each year support the fine conifer forest atop Palomar with old-growth trees including big-cone Douglas fir, white fir, reddish-barked incense cedar, live oak and black oak, Coulter pine and yellow pine.
The black oak trees, which are the only oaks in San Diego County to lose leaves in the fall, coloring the landscape with gold, show off new leaves in a bright spring green color. Interestingly, when those new leaves begin to sprout, they are tinged a bronze-red.
You'll see all of these towering trees on all the trails on Palomar. The Doane Valley Nature Trail is a great introduction to the region with its downloadable nature guide to take along as it interprets numbered posts on the 1-mile loop. The Thunder Spring Trail wanders through some of the best fir and pine forests on the mountain. And the French Valley Trail is an all-time favorite for massive old oaks that have thrived here for hundreds of years as well as wide-open meadows that surround a creek.
I've enjoyed hiking on almost every mile of the dozen trails in Palomar Mountain State Park. There are a few other tree-rich trails to explore on the adjacent Cleveland National Forest (CNF) lands, which include the observatory, operated by California Institute of Technology and open every day free of charge from 9 a.m. to 4 p.m.
When I went to Palomar Mountain State Park recently to hike there again, I planned to follow the Doane Valley to Fry Creek Trail, which connects the state park to the CNF Fry Creek campground. But checking in at the Palomar entry, the chief ranger, Jessica Murany, informed me that trail is all but obliterated, requiring more bushwhacking through poison oak than I like. She also noted that a family recently got lost (and found) in that area — enough information for me to seek a different route.
She directed me to Myers Fire Road, which proved to be another excellent foray through the forests of Palomar.
Leaving from the Doane Valley Campground — where some of the biggest and oldest oaks and firs make this a very popular camping destination — I followed Myers Fire Road uphill through thick stands of black oaks, incense cedars and pines.
I spotted the bright blue flashes of both western bluebirds and stellar jays. I marveled at those bronzed-red baby leaves of a young black oak sapling. I was astounded at the size of the pine cones of the Coulter pine — which is, naturally, also called big-cone pine. Coulter pine cones are the largest of any pine, measuring from 8 to 16 inches long and weighing up to 10 pounds. Little wonder they're called "widowmakers" if they fall on anyone's head.
In early May, wildflowers were showing off, too, including the purple chaparral pea, scarlet bugler, blue ceanothus (wild lilac), tiny yellow hairy lotus mixed in with white popcorn flowers, purple lupine, white blooming stems of the Western choke cherry, the short, low-growing blue Bajada lupine and lots of tiny baby blue eyes.
As you wind up the fire road, after a bit less than the first half-mile, you'll come to a faint road that goes off to the right; don't take that. It's an old driveway that goes to private property where fire claimed the home in the 2007 fire. Continue straight ahead on the Myers Fire Road.
After about 1.2 mile from your start, the main road makes a sharp hairpin turn to the right, while a fainter road continues at the left. Follow that hairpin turn to the right. In another half-mile or so, you'll reach the road's end where some old cabin ruins tell of a previous life up here. Turn around and go back the way you came. You'll note you've crossed from the state park into CNF lands on this road.
If you now take that faint road you passed at the hairpin turn, this continuation of Myers Fire Road dips downhill and in about another three-quarters mile, ends at a gate.
The views from Myers Fire Road are another bonus. On a very clear day, you can probably see the ocean. On any other day, you have up-high views down onto Palomar itself — the meadows and distant ridges covered in pines.
Myers Fire Road is the only trail in the state park where you can take your dog, always leashed. Dogs are also allowed on CNF lands, but not on state park trails (though they can be in state park campgrounds).
To date, more than $83,000 has been raised through private donations to help keep Palomar Mountain State Park operating. To keep abreast of that situation, and to donate, go to friendsofpalomarsp.org.
Priscilla Lister is a freelance writer from San Diego.Copyright © 2017, The San Diego Union-Tribune


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About the Book
This book is about the color green and how it relates to nature and sustainability. I made it in my Color Workshop class at Pratt Institute. Inside you will find, drawings, photographs, color theory and other information that relates to the color green.
Features & Details
- Category Arts & Photography
Small Square, 7×7 in, 18×18 cm
54 Pages
- Publish Date Nov 04, 2011
- Tags pratt institute, color theory, color transparencies, complementary colors, nature illustration, color textures, green, sustainability, colors, nature, ocean, photography, painting, watercolor, tikal, illustration, guatemala, flowers
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Order your ARC 2014-2015 Salon Catalogue
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Broader scope
by Virgil Elliott
Piet Spijkers wrote:
Virgil, then you must be quite familiar with the studies by Van de Wetering I suppose. Good to hear about
. The Utrecht Caravaggists are among my favourites, among them somebody seldomly talked about, Jan van Bijlert.
Yes, I bought Van De Wetering's book when it first came out, and have been following the activities of the Rembrandt Research Project with great interest, and the scientific analyses that are being done of old paintings by the various museums, foundations, and conservation groups. I try to stay current with everything that comes out on the subject, as I have for many years.
Regarding Honthorst, his painting of
Christ Before the High Priest
is probably my favorite painting in the National Gallery, in London. There was a travelling exhibit of the Utrecht Caravaggisti that came to San Francisco a few years ago, and Honthorst was the star of the show, in my opinion, though there were quite a few others who were noteworthy as well, Van Baburen being one whose name pops into my mind immediately. There were so many excellent painters in Holland in the 17th century that it is no wonder all their names cannot be household words today, just from the sheer number of them being more than most people's memory banks could retain. Who has heard of Cesar Van Everdingen today? Or Jakob Ochterveldt, or
Willem Drost
, etc.? Holland has a rich tradition in painting, and I have the utmost respect for the 17th century Dutch artists, as I have for the last 48 years.
An earlier publication that covered some of the same ground as Ernst Van De Wetering was
Art in the Making - Rembrandt
which was based on the National Gallery's analyses of the
paintings in their possession. More testing and studies were subsequently done, which were reported in Van De Wetering's book,
Rembrandt - The Painter at Work
. I also follow the National Gallery Technical Bulletins for updates on their ongoing studies and tests on old paintings, as they are continually making new discoveries. Likewise the other museums and conservation science efforts.
No artist who has ever painted well has gone unappreciated by me. It has been a lifelong obsession of mine to study them all, to seek them out wherever I can find them and learn all I can from them through their paintings. I have learned much more in museums and at my easel than any instructor has ever taught me.
Virgil Elliott


text:
|Chekol, T
|Vough, L
|Chaney, Rufus
Submitted to: Environment International
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/15/2004
Publication Date: 8/15/2004
Citation: Chekol, T., Vough, L.R., Chaney, R.L. 2004. Phytoremediation of polychlorinated biphenyl-contaminated soils: the rhizosphere effect. Environment International. 30(6):799-804. Interpretive Summary: Phytoremediation uses plants to aid in biodegradation of xenobiotic (non-natural) organic compounds which require remediation. The present study was conducted to characterize the role of plants and soil microbes in biodegradation of PCBs in amended soils. Initially different grass and legume forage crops were evaluated for their ability to increase the extent of PCB degradation. The two most effective plant species, Reed Canary Grass and Switchgrass, were further evaluated in sterilized and non-sterilized soil, with both unplanted and planted treatments. Sterilization had little effect on the rate of biodegradation, while planting substantially increased the extent of biodegradation of PCBs in this soil. Planting these crops increased the density of soil microbes in the soil, and especially in the rhizosphere soil (soil within about 0.1-0.3 mm of the root surfaces). The present experiment provides a strong illustration of the role of plant species in increasing soil microbial ability to degrade xenobiotic organic compounds which contaminate soils. It is well known that root exudates and lysed root cells supply nutrients which aid growth of soil microbes. Plants may further aid biodegradation by improving soil structure, and improving oxygenation of the rhizosphere. Phytoremediation is increasingly recognized as a method which can achieve required soil remediation at far lower cost than the default soil removal and replacement with clean soil. Effective management of the crop growth and monitoring of the extent of remediation are needed to achieve optimum remediation rate and lowest costs.
Technical Abstract: The objective in the first phase of this study was to screen alfalfa, flatpea, sericea lespedeza, deertongue, reed canarygrass, switchgrass, and tall fescue for phytoremediation of polychlorinated biphenyl (PCB)-contaminated soil. During the second phase, the focus was rhizosphere characterization to optimize PCB phytoremediation. Aroclor 1248 (PCB) was added to soil at 100 mg kg-1 of soil. In the first phase, all of the plant species treatments showed significantly greater PCB biodegradation compared to the unplanted controls and the two most effective species were selected for further study. During the rhizosphere characterization study, soil irradiation did not affect PCB biodegradation, but planting significantly increased PCB biodegradation; 38% or less of the initial PCB was recovered from planted pots, compared to more than 82% from the unplanted control soils. Presence of plants significantly increased the biological activity (microbial counts and enzyme activity) of both irradiated and unirradiated soils. Greater bacterial counts and soil enzyme activity were closely related to higher levels of PCB biodegradation. The data showed that Aroclor 1248 biodegradation in soil seem to be positively influenced by the presence of plants and plant-bacteria interactions. Our results suggested that phytoremediation could be an environmentally friendly alternative for PCB-contaminated soils.


text:
Write 9 articles on wordpress having 250-300 words
Budget $10-30 USD
Write and publish 9 articles on wordpress.
You will also need to upload some images of whom links will be provided
Idea about what to be written will also be shared.
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text:
'Slumdog Millionaire' star Dev Patel has been reported to be filling the shoes of Zuko in place of Jesse McCartney who is knocked out from 'The Last Airbender' due to scheduling conflict.
Despite his earlier assurance to fans that he is putting his best foot forward for his role in "The Last Airbender", Jesse McCartney is no longer attached to the feature film adaptation. Of the matter, Variety reported the pop singer has to bow out from the project due to scheduling conflict with his touring dates.
Explaining McCartney's exit, director/writer/producer M. Night Shyamalan stated, "Jesse had tour dates that conflicted with a boot camp I always hold on my films, and where the actors here have to train for martial arts." The "Leavin'" singer was actually attached to Zuko character, who is the Fire Nation's evil prince.
Coming in to replace the 21-year-old is "Slumdog Millionaire" lead actor Dev Patel. About the choosing of the rising star, Shyamalan revealed that the 18-year-old was "already one of the guys I was interested in." The filmmaker then added, "Then I saw 'Slumdog Millionaire,' and the kid just grew in my eyes." By joining the project, Patel will be featured alongside "Twilight" actor Jackson Rathbone and newcomers Noah Ringer, Nicola Peltz and Jessica Jade Andres.
A live-action film version of Nickelodeon's animated series of the same name, "The Last Airbender" will see Noah Ringer as Aang, Dev Patel as Zuko, Nicola Peltz as Katara, Jackson Rathbone as Sokka and Jessica Jade Andres as Suki. Production is expected to begin in mid-March in Greenland for July 2, 2010 U.S. release. The movie is said to be a part of a trilogy.


text:
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