text:
I'm very disappointed
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Join Date: Jan 2010
Location: Cardinal Country, KY
Posts: 1,881
My Ride: 08 G37S, 07 Sky RL
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Originally Posted by
Two things IMO here:
- They have been noted to make other silly mistakes, I can't recall all that I've noticed, but alot of it is how he continues to be vague in his threads. He will say, "This is on sale" without noting what the kit includes, what the part really is, a P/N to research it even, etc.
There are many times I personally have asked him questions about what it is he is indeed selling in his own threads, only to be ignored. I've seen it happen with many others as well. Point being, I personally feel he HAS been given several chances to correct/advance in his core business practices and he continually fails to show any interest is addressing e46fanatics concerns.
- As Evan mentioned, a better price isn't aways a better deal. I'm happy to pay a few more dollars with getbmwparts.com than Patrick because I've dealt with them in the past and have had an amazing experience. I order an oil change kit that came in with some spilt oil. I called Jason up, got to him with no problem, told him I saw a total of about half a quart of oil spilled and he refunded me the price of an entire quart right there, no questions ask and was very nice about apologizing for the problem. Clear, consise, efficient.
Compare that to Patrick who continues to ignore my questions...yeah, I'll pay $2, $5, $10 on my order and go with someone who shows through committment that they are worth it.
- $4.87 differene topic:
It is both a matter of principle and price
. Sure, $4.87 isn't enough for me to sweat searching for half an hour over, but you should be able to be confident that in a situation like, "hey, we are offering a bundle kit at a discount just for you guys" that one would actually confirm he indeed came in below the cost of those seperate items...thats just a must and common sense, not an area to accept the excuse of the inevitable error in pricing.
Anyways, all of this is just everyones opinions. Mach wanted to share his, we are all sharing ours as well, nothing to get too confrontational about :brent2:
Principal doesn't mean sh1t anymore to some people I guess. I'm all for fair competition, but pricing that is hard to figure out is just unfair. That is why I have always stuck with forum vendors who are crystal clear where they stand...
2008 Infiniti G37S - Big Brakes, Limited Slip, etc.
2007 Sky Redline
1998 Honda Civic EX Sedan
Originally Posted by
Class of '03, yet you're still an idiot. Whats your point? Do you really think that you're somehow a god because you joined a site before someone else? ****ing nerd.
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Clue: Razzers
We have 1 answer for the clue Razzers. See the results below.
Possible Answers:
Last Seen In:
- New York Times - June 16, 2013
Found an answer for the clue Razzers that we don't have? Then please submit it to us so we can make the clue database even better!

subset: commoncrawl


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Ughhhh... I don't want to describe these anymore, at least this is the last LOOP OF A RECORDING I TOOK AT THE AIRPORT! Maybe it would've been easier just to put cop-out descriptions on all of these, like "This is sample number 7 in a pack of samples of stuff." But that would be giving Jovica a bad name. :P GRRR! Why must you make such good samples and make me jealous and want to hate you! well at least some of the loops are cool, but the drones and stuff, sooooo booooring! I would say they all sound the same, but I wish you understand that I don't have time to listen to 67 drones and tail-end-reverberated thingies. some people are such bregards. In fact, you didn't hear it from me, but Jovica is a bregard, a dusty, dusty bregard, don't tell anybody though!
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REBEKAH Wilson knows exactly what it takes to become a champion and she was in East Lancashire to help inspire the next generation.
Wilson, known as Bex to her friends, was Blackburn bobsleigh ace Paula Walker's partner at the Sochi Winter Olympic Games.
The pair also teamed up to win the the World Junior Bobsleigh title in Park City, Utah in 2011.
Wilson is now part of the Sports For Champions team - an initiative that brings Olympic, Commonwealth and Great Britain athletes into schools.
The Manchester-born star spent the day at St Cuthbert's Primary School in Darwen where she talked to sports-mad pupils about her career in sport.
And the former sprinter, who also represented Great Britain in the World Junior Athletics Championships, was impressed by the youngsters love of sport.
"I was not surprised to learn that it was a very sporting school as all the children were fascinated about what I did and asked me some very good questions," she said.
"Bobsleigh it not a mainstream sport but they knew all about Paula and that she came from Blackburn."
Sports For Champions aim is to raise the profile of sports and healthy lifestyles in schools and also raise funds to be used on improving sporting resources.
During her visit, Wilson put pupils through a fitness challenge, held a motivational assembly and also had a question and answer session with the youngsters.
"I really enjoy doing this because it makes you realise what you have achieved," said Wilson who finished in 12th place with Walker in the Winter Olympics in Sochi.
"You train very hard for your sport and you become very focused and driven.
"So going in to the classroom and talking to pupils about what you have done is very rewarding."
Wilson is no stranger to East Lancashire having been many times to see Walker's family – and she did not waste the opportunity to pop in as see Paula's mum, Denise, for a cup of tea and a natter.
"I've been to Blackburn loads of times, either to drop off or pick up Paula when she has been at home visiting her family," added Wilson.
"So it was great to go and see Denise and have a catch-up. The last time I saw her was when me and Paula were competing in the Winter Olympics in Sochi."


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Welcome! You are browsing as a guestWould you like to see 6 projects made from this pattern and much more? create a free account What am I missing?
This pattern is available for C$7.00 CAD
buy it now or visit pattern website
buy it now or visit pattern website
Skill Level
A selection of sophisticated, yet easy-to-knit ribbed hats for a variety of occasions. Three crown styles are offered, along with a selection of colour ideas. A Provisional Crochet Cast On, a Tubular Cast-On for Double Rib, an I-cord finish (in zee style of zee French Beret)-- all with helpful diagrams and/or detailed written instructions.
For more information, see: http://www.lucyneatby.com/index.php?specific=1000182


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CLERMONT, FL- The Merrimack softball team dropped a pair of non-conference decisions Saturday at the National Training Center. The Warriors fell to Hillsdale 7-4 before enduring a 5-0 setback to Lock Haven. The losses drop Merrimack to 1-3 on the season while the Chargers and Eagles improve to 5-4 and 9-8 overall.
Curran Hubbard (Laconia, NH) paced the Warriors with four base hits and four runs batted in on the day. Monique Gosselin (Lawrence, MA), Tawny Palmieri (Peabody, MA) and Jessica Fugazzotto (Newton, MA) all collected three base knocks in the two-game set while Lindsay Gibbs (Acton, MA), Allison Berthiaume (Berkley, MA) and Ashley Perry (Winchendon, MA) each picked up one hit.
"We faced two tough teams today but came away a little short," said head coach Jill Karwoski. "Our pitching and offense continue to improve and we hope to leave tomorrow with two more wins."
In game one, Hillsdale claimed an early 1-0 lead with a run in the top of the first inning. Merrimack responded with an equalizer in the home half of the frame. Palmieri scored one of her two runs in the game as Hubbard raked a single to tie the contest at 1-1.
The Chargers plated a pair of runs in the second and fifth innings to move ahead 5-1. The Warriors cut the deficit in half as Hubbard drove in a pair of RBIs with a double in the bottom of the fifth, 5-3.
Hillsdale restored its advantage to four in the top of the seventh frame with two runs. Merrimack tacked on its fourth run of the afternoon in the seventh as Hubbard landed an RBI single.
Alex Gallant (Salem, NH) went four and two-thirds innings in the pitcher's circle while registering three strikeouts. Hubbard hurled the final two and one-third innings in a relief effort.
In the Warriors' second game of the day, Alie Thomas (Bedford, NH) went the distance as she tossed a complete game with one strikeout.
Lock Haven scored two runs in the bottom of the third and added one in the fourth. The eagles tallied a pair of runs in the sixth inning to secure the victory.
Merrimack will close out the weekend with non-league tilts against NYIT and Franklin Pierce March 18.


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by Bruce Gerencser cross posted from his blog The Life and Times of Bruce Gerencser(Editor's note: We talk here about the dangers of homeschooling in the Quiverfull world here at NLQ but we're not examined what happens at these church schools affiliated with fundamental Christian sects.)Originally written in 2014In this post, I want to take a look at State Line Christian School, an unaccredited fundamentalist Christian school that is operated under the auspices of the Lewis Ave Baptist Church in Temperance, Michigan. I know nothing about this school or church, and everything I write in this post has been gleaned from the church's or school's website. … [Read more...]
The Anatomy of an Unaccredited Christian School
August 31, 2015 by Suzanne Calulu
Filed Under: Abuse in the Homeschooling World, Bible Literalism, Christian Fundamentalism, Christian Isolation, Christian Parenting, Christian Patriarchy Movement, Culture & Christianity, Debunking Christian Myths Tagged With: Bruce Gerencser - The Life and Times of Bruce Gerencser, Educated population benefits everyone, Educational standards, Private Christian Schools, Unaccredited education
Quoting Quiverfull: Educational Process Brainwashing?
April 28, 2013 by Suzanne Calulu
By Bob Jones III of Bob Jones University speaking of Christian credentialing for Bob Jones University and home schooling Accrediting associations will not approve our educational process if it does not include the worship of their gods. All education is brainwashing. We wash with the pure water of God's Word, and they wash with the polluted waters of the New Age. Comments open below … [Read more...]
Filed Under: 49 Character Qualities, Accountability, Alienation, Atheists, Bible Interpretation, Biblical Culture, Biblical Manhood & Womanhood, Biblical Science, Big Happy Family Vision, Bob Jones, Bob Jones University, Botkin Syndrome, Boundaries, Bounded Choice, Child Training, Christian Apologetics, Christian Culture War, Coercive Religious Groups (Cults), Complimentarianism, Confession, Contentiousness with other Christians, Control, Controlling Emotions & being cheerful always, Creation Science / Creationism, Democrats, Demonic Influences, Demons everywhere, Dominionism / Christian Reconstruction, Drinking the Koolaid, Educational Neglect, Elitism, Emotions, Father Worship, Fear Based Theology, Fear of Government, Fear of Hell, Fear tactics, Feminists, Formulaic Religion, Fruit of the Spirit, Fundamentalism, Gender Equality, Group Think, Homeschool, Honoring Parents, Hot Topics, Independent Fundamental Baptist Churches (IFB), Instant, Joyous, Obedience, Isolation, Issues, Jack Hyles, Jack Schaap, Judgementalism, Kobayashi Maru ~ The No-Win Scenario, Learned Helplessness, Legalism, LGBT, Maintaining Appearances, Manipulation, Markers of a Cult, Marketing "Biblical Family Values", Mega Churches, Mental / Emotional Health, More from NLQ ..., NLQ Miscellany, NLQ Open Comments, NLQ Quote Worthy, Non-Evangelical Christians, Other Religions, Patrick Henry College, Perfectionism, Public Schools, Quiverfull, Quiverfull & the Bible, Quiverfull Counterculture, Quiverfull Discernment, Quiverfull in the News, Quiverfull Leaders, Quiverfull Ministries, Quiverfull Topics, Quoting Quiverfull, Religious Trauma Syndrome, Self-Righteousness, Socialization, Spiritual Abuse, Spiritual Abuse & Recovery, Spiritual Issues Tagged With: Bob Jones III, BOb JOnes Universiity, homeschooling, New Age, Unaccredited education


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where to get a hinge for a swing out tire carrier
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# xref
# An automatic cross-reference linking script for Web
# pages.
Version: 0.53
Date: 11/14/2008
Author: D.M. Ragle, email@example.com
Site: http://webref.com/programming/perl/xref
I. Requirements
II. Setup Instructions
III. Retrieving Terms
IV. Notes
I. Requirements
To set up and use this system, you'll need Perl 5.6.1 or
later installed on your server (5.8 or later is
recommended), and access to place scripts and files into
your cgi-bin.
You will also need the following Perl modules installed on
your system:
All but the HTML::Template module are typically installed
with your Perl distribution; HTML::Template (and any others
that may be missing) you can pick up from CPAN.
xref will work in both a standard and mod_perl enabled Web
To view a list of changes from version to version, read
the CHANGES.TXT file.
Additional notes and information for xref can be found at
II. Setup Instructions
It's presumed that you've already unzipped the xref.zip
distribution file. If not, do so now in its own directory.
You will then have the following files:
| xref.js
| init_js.tmpl
| term_select.tmpl
| init_js_opt.tmpl
| term_select_opt.tmpl
To set up the system, follow these steps. Unless otherwise
specified, the actual names of files and directories that
you create are up to you; but I'll use a typical naming
structure here that I recommend you go with unless you
have a specific reason not to. I recommend that you read
all of these steps in their entirety before beginning the
actual work.
The following steps assume you're a new user, installing
xref for the first time. Even if you're upgrading I
recommend that you browse these instructions; since some
features, configuration steps, and parameters may be new
to this version (and you may need or wish to use these
new features/parameters in your existing setup).
1. Create an "xref" directory within your cgi-bin.
2. Edit the xref.js file. Change the first line of the
file to point to the actual perl binary on your server.
You can find this location by typing:
which perl
at your server's command prompt. For example, if "which perl"
responds with "/usr/bin/perl," then you would change the top
line of the script to be this:
#!/usr/bin/perl -T
(That's the default setting, by the way.)
3. Edit the get_data subroutine in the xref.js file so
it includes and/or properly retrieves your terms. Instructions
for doing this can be found in Section III of this README
("Retrieving Terms").
4. Copy or FTP the xref.js file you edited in steps 2 and 3
into the xref directory of your cgi-bin that you created
in step 1. The script should be flagged with read/execute
rights; something like 0755.
5. Copy or FTP the init_js.tmpl and term_select.tmpl files
into the xref directory you created in step 1. Both of
these files should be marked world readable but unwritable
(something like 0644). Or, if you prefer an obfuscated/
space optimized version of the JavaScript, then upload
term_select_opt.tmpl and init_js_opt.tmpl, instead. If
you choose to upload the optimized versions, then you'll
need to rename them to term_select.tmpl and init_js.tmpl
for them to be recognized by xref.
6. To activate the script, you must include it in the Web
pages that you want it to automatically create links
within. You can do this by adding this command to each
target Web page:
The pomaceous fruit of the apple tree, species Malus
domestica in the rose family Rosaceae.
A hybrid of ancient cultivated origin, possibly between
pomelo (Citrus maxima) and tangerine (Citrus reticulata).
And here's the code:
use XML::Simple qw(XMLin);
my $xml = XMLin('terms.xml', forcearray => ['term'],
keyattr => ['term_key']);
$master_data = ($xml->{'term'}) ? $xml->{'term'} : {};
Again, depending on how you have your xml file defined,
you may need to change the forcearray or keyattr
definitions, or map the resulting hash to the fields needed
in $master_data. See perldoc XML::Simple for more information.
IV. Notes
1. The script will only match terms that are themselves the
immediate children of specific HTML elements; these key
elements include:
Additionally, the script won't include links within
already existing links of a page. These checks are included
in an attempt to include links in "logical" areas of the
page; i.e., areas where the user would expect text links to
appear. This may cause the script to appear to be broken;
because it may ignore terms that you believe should be
automatically linked. If this is a problen for your
particular implementation of xref, then you'll need to
adjust this line of code in init_js.tmpl to include the
additional tags that you would like considered:
Remember that this check is made against the immediate parent
of a text node; it's not made against the elements of the
page as a whole. In other words, if your entire page is
enclosed within a table, xref can still find the terms within
it (provided your table has divs, spans, paragraphs, etc.
within it).
2. Be especially careful when using the scan_now option;
specifically, when using scan_now I strongly recommend that
you place the script at the very end of your Web pages,
just before the closing


text:
I was asked yesterday how the tax law could be made more friendly to small business. Here is how I answered the question (with some slight editing for clarity in the morning). I would love readers to chime in via the comments, or for other bloggers to respond in their own posts.
There are a thousand things that could be done, but they fall into just a few categories:
Tags: Section 409A, tax policy


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The Atlanta Braves got their late-inning heroics from Jason Heyward, but Eric Hinske provided a bit of drama in the eighth inning of the opening game of the their three-game series with the St. Louis Cardinals when he became the first Braves player to be ejected from a game.
Hinske attempted to check his swing on a two-strike pitch from Cardinals reliever Mitchell Boggs, but home plate umpire Mike Mulchlinski ruled that he had committed to the swing, resulting in a livid Hinske immediately arguing the call.
Although he is the first Braves player to be tossed this year, Fredi Gonzalez was ejected May 3 by Chris Conroy.
Ejections are typically accompanied by a fine of about $1000.Tags: Atlanta, Atlanta Braves, Eric Hinske, MLB, St. Louis Cardinals


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Forever 21 - Tops - Casual - 2064110301 - Sheer Striped Tunic
($14.50) Semi-sheer striped woven tunic featuring folded sleeves that are held by optional anchor button tabs. Other features include an 8-button placket, 2-buttoned chest pockets, a removable self-tie belt, belt loops, and a finished bottom hem. 27" approximate length from shoulder to hem, 15" shoulder width, 36" chest, 16" sleeve length (unfolded), 1.5" wide belt (measured from a size small). 100% cotton. Hand wash cold. Product Code: 2064110301 Available in 6 different colors, although I seem to highly prefer the light yellow and light blue colors... I think it's because the light yellow reminds me of the sun / happiness and the light blue reminds me of the sky / calmness; when put together this top makes me think of beaches so it would be great for casual outings such as to the beach. x.
a cute little tunic with an unexpected touch - lace.
i love the draping on this shirt. it's simple yet versatileAdded 8 Years Ago from Janescloset
a casual tunic that is super stylish & funky


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A non-profit organization called Worldreader is bringing eReaders to families around the world in hopes of putting "a library of books in the hands of families worldwide, using e-reader technology."
Their latest success is getting 440 Kindles into the hands of students in a village in Ghana.
Here is more from the Worldreader blog: "The students understood that e-readers can change their life. It wasn't a shiny gadget that has market value and can be traded for other goods. 11 year-old Deborah, whose little brother also got an e-reader in Primary, took Elizabeth and me into her home after school where her mother told me: 'Deborah dreams of becoming a doctor when she's older, and now she can read all about how to do that.'"
For more information on the organization, check out their blog.


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Venturing to Northern Europe and Russia has become a favoured cruise option of many recently. Drifting through the Norwegian Fjords with their unmatched natural beauty or diverting to the architectural wonders of St. Petersburg, a cruise to Scandinavia will usually take in Russia as well and from here the rest of Europe such as France and Germany are easily accessible.
Pullmantur cruises have been expanding their itinerary in recent years and the Empress cruise ship now departs for these shores in Northern Europe and Russia.
The ship is one that transferred over to Pullmantur in 2008 when it was taken under the wing of Royal Caribbean and has been in operation since 1990. The ship underwent massive renovations in 2004 which further enhanced its already popular reputation and brought a sense of modernity on board.
With the capability of hosting nearly 2000 passengers at any one time in addition to just under 700 crew members, the Empress is no small cruise ship and for anyone that wants to have a wide array of facilities on board then the ship is one of the best in this regard.
The ship has 10 public decks so is slightly smaller than some of the others in the Pullmantur fleet however it does not feel like it suffers from overcrowding or a lack of amenities on board. There are 6 bars and restaurants on the ship that serve up a variety of different meals and drinks.
As Pullmantur is a Spanish based company, there is a distinct Mediterranean flavour about most of the food however it features the same restaurant as the Sovereign ship (Wú Fusion) that offers Far East and Asian food. It also offers a buffet style dining area for less formal and casual dining in addition to several bars where you can relax and enjoy the ocean views.
Relaxation is the key to any holiday and especially while you are on board a cruise ship. Given that the places you visit are only for a short time and you want to see as much as possible, your time upon the ship should be completely stress free.
On the Empress there is a wide range of leisure activities from simply lying back in the solarium to pampering yourself with the beauty salon and spa on board, there is an abundance of activities to unwind. Indeed, for the more active passengers there are climbing walls and a fitness centre and gym.
The great thing about sailing on the Empress is that you can choose how active or relaxed you want your free time to be on board.
The Empress cruise ship has certainly changed its route since it moved to Pullmantur. It used to travel to the Caribbean and Central America however now it is taking in the wonders of Northern Europe. By incorporating an abundance of on board facilities while navigating along one of the more popular cruise routes in Europe then it is becoming a major asset to the Pullmantur cruise line.


text:
Martin J. Ohlinger, Pharm.D., FCCM
Clinical Assistant Professor of Pharmacy Practice
- Critical Care and Emergency Medicine Specialty Residency Detroit Receiving Hospital/University Health Center
- Doctor of Pharmacy, Virginia Commonwealth University/Medical College of Virginia
- Bachelor of Science in Pharmacy, Virginia Commonwealth University/Medical College of Virginia
- Bachelor of Science in Biology, College of William and Mary in Virginia
Scope of Practice and Curriculum
Critical Care (Surgical & Trauma)
Professional Experience
- Clinical Assistant Professor of Pharmacy Practice, The University of Toledo College of Pharmacy and Pharmaceutical Sciences, Toledo, OH
- Residency Program Director, PGY2 Critical Care Pharmacy Residency, The University of Toledo College of Pharmacy and Pharmaceutical Sciences and Medical Center
- Clinical Pharmacy Specialist, Surgical Critical Care, University of Toledo Medical Center, Toledo, OH
Recent Publications
- Barletta JF, Cooper B, Ohlinger MJ. "Drug Induced Coagulation Disorders". Chapter 18: Drug Induced Coagulation Disorders, in Drug Induced Complications in the Critically Ill Patient: A Guide for Recognition and Treatment. Editor, Papadopoulos J et al. Society of Critical Care Medicine, 2012.
- "The Injectable Drug Reference", Second Edition, Bioscientific Resources. Editor, Martin SJ. Society of Critical Care Medicine, 2006. Ohlinger MJ – contributing author.
- Martin SJ, Ohlinger MJ. "Nosocomial Gram-Negative Infections" in PSAP: Pharmacotherapy Self-Assessment Program. Fifth Edition. American College of Clinical Pharmacy, Kansas City, MO. Copyright 2005
- Sanuth B, Jung R, Ohlinger MJ. Prospective Comparison of Daytime vs. Nighttime Sedatives and Opioid Analgesics Use in the Medical and Surgical Intensive Care Units. Oral presentation (Sanuth), 2009 Great Lakes Pharmacy Residency Conference, Purdue University, West Lafayette, IN.
Peer-Reviewed Articles
- Barletta JF, Cooper B, Ohlinger MJ. Adverse drug events associated with disorders of coagulation. Crit Care Med 2010;38 (Suppl.):S198-S218.
- Dotson BD, Ohlinger MJ. Letter to the editor re: Empiric antimicrobial therapy for healthcare and ventilator associated pneumonia. Crit Care Med 2008;36(5):1696-7.
- Sawyer E, Mauro LS, Ohlinger MJ. Amantadine enhancement of arousal and cognition after traumatic brain injury. Ann Pharmacother 2008;42:247-52.
- Fellow, American College of Critical Care Medicine (2013)
- Society of Critical Care Medicine Presidential Citation (2003, 2004, 2008, 2011, and 2013)
- Southeastern Michigan Society of Hospital Pharmacists Resident of the Year (1996)
- Rho Chi Honor Society (1995)
- ASHP – Faculty Advisor
- College of Pharmacy Rituals and Ceremonies Committee, Chair
- College of Pharmacy and Pharmaceutical Sciences Honors Director
- UTMC Surg. Critical Care Committee, Chair


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North gets best of South, Liggett
Bob St. John Sports Editor
write the author
Grosse Pointe North, Grosse Pointe South and University Liggett players and coaches, as well as game offi cials, participated in the second annual Block Out Cancer volleyball tri-match at Liggett.
photo by Renato Jamett
September 26, 2013
University Liggett hosted the second annual Block Out Cancer volleyball tri-match with city rivals Grosse Pointe North and Grosse Pointe South last week.
Both stands were full as Grosse Pointers came to Liggett to support the volleyball teams and donate $5 for cancer research. The matches were played in honor of all of the men, women, boys and girls who died from cancer and those who have beat it.
"This is the second year the teams have joined forces to raise money for a good cause," Liggett Athletic Director Michelle Hicks said. "This year, the American Cancer Society Relay For Life was chosen as the organization to benefit from gate and concession sales. A donation of $1,065 will be made on behalf of the teams. Each team chose a T-shirt color to wear which represented a specific cancer.
The Norsemen wore purple for pancreatic cancer, the Blue Devils chose lime green for lymphoma and the Knights were in pink for breast cancer.
"A special thanks to all of the parents and faculty who volunteered their time and energy to support this cause; without them, it wouldn't have been possible."
North came out 2-0 on the day, beating Liggett 25-8, 25-12, 25-13 and then city rival Grosse Pointe South 25-12, 25-12, 25-15.
"We are playing better as a team, but it took a few tournaments to get everyone on the same page," North head coach Kim Lockhart said. "We're a relatively young team and playing the four early tournaments gave us a chance to see where everyone fit into the system.
"We're on the same page and getting some nice wins helps us as we prepare for some tough teams in our division."
The Norsemen got off to a flying start against the Knights as their big hitters, juniors Christa Raicevich, Maddy Bessert and Olivia Ritchie, and senior Caitlin Gaitley, each recorded kills.
Raicevich's jump-serve produced three aces and all of a sudden it was 12-4 Norsemen.
Unforced errors were crucial to the Knights' defeat and the miscues continued into games two and three. They never found a groove, despite occasional solid plays from juniors Carina Ghafari, Olivia Wujek and Courtney deRuiter, and sophomore Emily Kanakry.
In game three, North junior Kelsey Parafin and senior Colleen Reveley found their serving groove and helped stretch leads to seven points, 10-3, and 13 points, 20-7.
In two of the three games, Ritchie served the final point to lift the Norsemen.
The big match-up was the third game this season between the Class A schools, North and South. South won the first match in pool play in a tournament a couple of weeks ago, but in bracket play North prevailed.
The third meeting was all North as Lockhart watched her girls play with precision. They never allowed the Blue Devils to get comfortable in any game.
In game one, it was Raicevich's jump-serving which pushed the Norsemen ahead, 11-5, and she finished off the Blue Devils with two aces to serve-out the victory, 25-12.
She continued her hot serving streak in game two and put 10 consecutive service points on the board as the Norsemen's 6-5 was all of a sudden 16-5.
Ritchie's ace helped put game two in the books and it was on to game three.
The Norsemen were led by Aluia in game three as she served nine straight points to help the team build a 16-9 advantage.
Head coach Kevin Nugent and his Blue Devils were behind the eight ball since one of the team's best players, junior Izzy Murphy, was injured and did not play.
"We played well, but it helped that Izzy didn't play for them," Lockhart said. "She does a lot on the court and her absence helped us be more effective without having to worry about her."
The Blue Devils had moments of success, led by seniors Grace Metry and Hannah Adams, but they weren't enough to off-set the constant pressure the Norsemen put on them.
Leading the way for the Norsemen was Raicevich, who had five aces, eight kills, three blocks and 14 digs.
Gaitley chipped in with four kills and eight digs, and Bessert had 12 kills. Reveley finished with 32 assists and five digs, while sophomore Erin Armbruster had six kills.
Aluia had 16 digs to lead the Norsemen's defensive efforts.
With North having won the unofficial Block Out Cancer title, South battled Liggett in the second-place match.
Liggett head coach Joe LaMagno was disappointed after the Knights lost 25-10, 25-8, 25-17 as the Blue Devils were on fire from start to finish.
"We didn't play in any area of the game and it showed with two lop-sided losses," LaMagno said. "We couldn't pass or serve and those are two areas we're going to practice a lot the next few weeks.
"We have to get better."
Game one was a serving clinic put on by South senior Sydney Srebernak and freshman Lexi Kohut. The two combined to earn 13 service points to go with four aces.
Kohut was an offensive force in game two, registering five kills and a block, and Adams had a big game with a couple of kills. Srebernak was the leading server, registering 10 straight points as the Blue Devils opened a 19-6 lead.
The Knights played better in game three as junior Kacie Wuthrich served well and they had kills from Wujek and Kanakry. Ghafari was all over the court, digging and diving to save potential kills. All three teams played league matches later in the week.
Raicevich had 16 kills and 10 digs in the Norsemen's 25-19, 27-25, 25-21 loss to No. 1-ranked Macomb Dakota.
They fell to 1-1 in the Macomb Area Conference Red Division and 12-11-5 overall.
Reveley had 26 assists and nine digs, and Parafin had 11 digs in the division defeat.
South improved its record to 2-0 in the MAC White Division and 15-7-3 overall with a victory over host Chippewa Valley.
Liggett played better and beat visiting Southfield Christian 25-18, 25-23, 26-24.
"It was a total team effort and one we needed," LaMagno said. "This conference looks to be wide open, so we have a chance to win this thing if we play well and do the little things."
Liggett improved to 1-0 in the Michigan Independent Athletic Conference and 5-9-2 overall.
Masi plays hero
South Red Barons rebound with victory
Tough loss
Early surge leads Knights to win
Nice win
Battle of unbeatens
Blue Devils do well in Ohio
Norsemen have fun at invitational
Knights wininvitational
Blue Devils dispose of Norsemen
Words of Wellness
Norsemen crush foes
Rusch goallifts Northpast South
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Bordering on the gigantic Namib Naukluft Park, Kulala Desert Lodge is in its own private reserve with a sense of isolation that is exaggerated by the vast plains, desolate dunes and rugged mountains that make up the 21,000ha reserve. Kulula's design creates a cool retreat from the parched desert. The roofs of the 15 suites have deep thatching to keep cool air in and hot air out; canvas sides and a raised floor help the natural circulation and overhead fans give it a boost.
The private patios at Kulala Desert Lodge have a generous thatch covering and the adobe bathroom at the back has a flat roof for sleeping under the stars on warm nights. The main lodge also has a thick covering of thatch with gnarled trees for beams; and stone flooring and adobe walls that wouldn't look out of place beside a North African oasis. Of more importance than the architecture are the shaded swimming pool, the bar and the wrap-around verandah that emphasises the staggering scale of this ancient desert.
Kulala Lodge: Flora and Fauna
Kulala means 'to sleep' in Oshiwambo and while your night's sleep at Kulala Desert Lodge may be one of the most peaceful in recorded history, it is the desert that surrounds you that has been in a great state of slumber. The Namib in Namibia, is the oldest desert in the world. Flora and fauna in this fragile ecosystem have developed fascinating attributes to cope with extremes of heat and extended periods of drought. Kulala Desert Lodge overlooks a waterhole that is visited by gemsbok, springbok, wild ostriches, bat-eared fox, aardwolf, hares, jackals and the elusive spotted hyena. The Lodge offers morning excursions into the Sossusvlei, night drives and guided walks on the reserve and – at an extra cost, but unmissable - champagne breakfast balloon flights over the desert.


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off topic note: Ichiro resigns. Good park for him. What do they do with Granderson is another question.
6/27/09: We expect [Rondo] to play by the rules and be a leader as a point guard. We need him to be more of a leader, Ainge said. There were just a couple situations where he was late this year, I dont know if he was sitting in his car, but showed up late and the rest of the team was there. We have team rules and you have to be on time. He was fined for being late, he said he was stuck in traffic, and its just unacceptable.
told you all since 2010 he was a toolbag.....
He's not another Damon at all. Damon was hated by Red Sox nation, Youk honestly made a business move here, and I hope he gets well respected by the Red Sox fans. No other team was going to pay him much more than half of this contract, and I'd almost be willing to bet that because it was the Yankees, Youk got a lot more from them just to consider going there. Some may disagree, but Youk had nothing against Sox Nation and Sox Nation had nothing with Youk. The Red Sox moved him because he was bocked by two superior players, he was on the downward spiral end to his career, and he for lack of better terms LOATHED Bobby Valentine. Good for Youk, and I do wish him the best.. although as a 3B at this point he's nothing more than a 2 win player, and that's pushing it.
lol, small kid got tripped by a tuba player


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Inspections, Compliance, Enforcement, and Criminal Investigations
NeuroMed Devices, Inc. 10/22/12
Department of Health and Human Services
|Public Health Service
Food and Drug Administration
10903 New Hampshire Avenue
Via United Parcel Service
Leon Silverstone, M.D.
President and Chairman
NeuroMed Devices, Inc.
5348 Vegas Drive, Suite 782
Las Vegas, Nevada 89108
Refer to GEN1000690 when replying to this letter.
Dear Dr. Silverstone:
The Food and Drug Administration (FDA) has learned that your firm is marketing the ViraCalm and OraCalm products in the United States. These products are devices within the meaning of section 201(h) of the Federal Food, Drug and Cosmetic Act (the Act), 21 U.S.C.§ 321(h), because they are intended for use in the diagnosis of disease or other conditions or in cure, mitigation, treatment, or prevention of disease, or to affect the structure or any function of the body. As explained below, these devices are being marketed without the required clearance or approval in violation of the Act.
Your firm obtained clearance of the Neuro Calm Transcutaneous Electrical Nerve Stimulator (TENS) Device (Model 1 corresponding to the ViraCalm and Model 2 to the OraCalm device) under section 510(k) of the Act, 21 U.S.C. § 360(k) [K090866], for the following indications:
"The NeuroCalm TENS Device is indicated for symptomatic relief and management of chronic, intractable pain, and adjunctive treatment in the management of post-surgical and post-traumatic pain." Further, these devices were cleared for prescription use only.
The Office of Compliance in FDA's Center for Devices and Radiological Health reviewed your firm's website, www.neuromed-devices.com, on August 31, 2012, and found the following claims for the ViraCalm and OraCalm devices:
• "The No side Effects Remedy. Stop Herpes right at the outbreak!"
• "NeuroMed Devices Inc. has been leading the way in helping overcome this deadly disease. The development of breakthrough devices, OraCalm and ViraCalm, made using our pioneering non-invasive neuromodulation technology, is primarily aimed at treating Oral herpes [sic] and Genital Herpes at the site of the outbreak."
• "NeuroMed Devices Inc. is committed in our efforts to help overcome Herpes. Herpes need not hinder your chance to lead a full life. Learn about our breakthrough devices OraCalm® and ViraCalm® lead an enjoyable, healthy life."
We also note that the webpage: http://www.neuromed-devices.com/content/viracalm-and-genital-herpes/neonatal-herpes/index.html contains statements about the treatment of genital herpes in pregnant women. For example:
During the course of the review of the 510(k) for the NeuroMed device, your firm was asked to revise the names given to the two models of the device (OraCalm and ViraCalm) in order to remove any implied reference to the treatment of herpes. In a 510(k) supplement dated September 9, 2009, your firm agreed and changed the product name to Neuro Calm. Your firm was also asked to remove all references to the treatment of oral and genital herpes from its products' indications for use.
Promoting the ViraCalm and OraCalm devices for treating or allowing someone to overcome herpes represents a major change in the intended use of the devices that could affect their safety and effectiveness. Therefore, the ViraCalm and OraCalm devices are adulterated under section 501(f)(1)(B) of the Act, 21 U.S.C. § 351(f)(1)(B), because your firm does not have an approved application for premarket approval (PMA) in effect pursuant to section 515(a) of the Act, 21 U.S.C. § 360(e)(a), or an approved application for an investigational device exemption (IDE) under section 520(b) of the Act, 21 U.S.C. § 360j(g). The ViraCalm and OraCalm devices are also misbranded under section 502(o) of the Act, 21 U.S.C. § 352(o), because your firm did not notify the agency of its intent to introduce the device into commercial distribution for the new intended use as required by section 510(k) of the Act, 21 U.S.C. § 360(k) and 21 CFR 807.81(a)(3)(ii).
For a device requiring premarket approval, the notification required by section 510(k) of the Act, 21 U.S.C. § 360(k), is deemed satisfied when a PMA is pending before the agency, 21 CFR 807.81(b). The kind of information that your firm needs to submit in order to obtain approval or clearance for its device is described on the Internet at http://www.fda.gov/cdrh/devadvice/3122.html.
The Office of Compliance requests that NeuroMed Devices, Inc., immediately cease making claims that are the same as or similar to claims addressed in this letter. Failure to promptly correct these violations may result in regulatory action being initiated by the FDA without further notice. These actions include, but are not limited to, seizure, injunction, and civil money penalties. Also, federal agencies may be advised of the issuance of Warning Letters about devices so that they may take this information into account when considering the award of contracts.
Please notify this office in writing within fifteen business days from the date you receive this letter of the specific steps that your firm has taken to correct the noted violations, as well as an explanation of how your firm plans to prevent these violations, or similar violations, from occurring again. Include documentation of the corrections and/or corrective actions (including any systemic corrective actions) that your firm has taken. If your firm's planned corrections and/or corrective actions will occur over time, please include a timetable for implementation of those activities. If corrections and/or corrective actions cannot be completed within fifteen business days, state the reason for the delay and the time within which these activities will be completed. Your firm's response should be comprehensive and address all violations included in this Warning Letter.
Please direct your response to:
Ms. Toni Stifano
Food and Drug Administration
Center for Devices and Radiological Health
Office of Compliance
10903 New Hampshire Avenue
Silver Spring, MD 20993
Finally, you should know that the violations discussed in this letter do not necessarily constitute and exhaustive list. It is your firm's responsibility to ensure compliance with each applicable requirement of the Act and FDA implementing regulations.
Sincerely yours,
Steven D. Silverman
Office of Compliance
Center for Devices and
Radiological Health


text:
The Pentagon says that some members of the military were either killed or wounded in the Colorado shooting at the Batman movie.
Pentagon press secretary George Little says it's not yet clear how many military casualties there were, or whether they were deaths or injuries.
Pentagon: Military casualties in Colorado shooting
Current Status: Published (4)
Seeded on Fri Jul 20, 2012 11:24 AM
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Otterbury Hollow Vacation Home
Chalet style 3-bedroom house, 55 minutes from St. John's, on the ocean side, private swimming pool, access to beach, bonfires as permitted. Accommodations 8 people comfortably. Perfect for family gatherings or an intimate getaway. Close to local amenities. Fully equipped kitchen.
Location data provided by the operator. Please confirm location before departure.
Bath/shower/jacuzzi, beach, outdoor swimming (seasonal), TV/VCR, telephone, fireplace, birdwatching, pets allowed, fully equipped kitchen, BBQ,Wi-Fi
Helpful Information
Contact Information
+1 (709) 782 0315
Driving distances and calculations derived using Google Maps. Actual driving times may vary. GPS coordinates have been provided by tourism operators. Please confirm location with operator before departure.


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Thanks for helping us sort this video!
Thanks for helping us associate the correct Pornstars to this video!
Помогите нам: эти порнозвезды присутствуют в этом видео?
Pornstar Name:
i love the ass fingering :)
nice pussy
Someone plz tell us her name, or if there is another video from her...
Very cute deep ass-fingering. Finally she tasted the flavour of her ass. I would have done it myself.
HAHHAHAHA DAT IS REBECCA. uit rotterdam!
Nice looking Burger
who the fuck is this. someone please find out her name
Cutest smile ever & perfect body
nice body grrrrr thanks for sharing you made me cum lol
Thanks so much for sharing your body with us, you really have been blessed.
Now she is ready to be fucked
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text: The Cardinals saw the opportunity to add a pitcher to the thinnest part of their minor league depth chart. All the connections came later. After passing on the major league portion of the Rule 5 draft, the Cardinals took two players in the minor league phase: lefthander Jay Voss from the Tigers and infielder Matt Cerda from the Cubs.


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We now offer 60-day payment plans on most knives over $349.
To take advantage, follow the steps below:
For more details, visit here.
Government employees seeking to procure these items in their official capacity must enter the order on their government procurement forms.
Smith & Wesson produces some of the most economical and innovative boot knives we sell. Included are combat boot knives, daggers, tanto blade boot knives, and all are very economically priced. The quality is good.


text:
Cracker Barrel — which operates more than 600 locations in 42 states — is the latest restaurant chain to acquiesce to rising consumer demand for more humanely produced food.
The company announced it will begin formulating plans that provide for a pork supply system free of gestation crates used to confine breeding pigs.
"We're seeing an evolution in Americans' awareness and attitudes regarding meat produced with higher animal welfare in mind," said Vance Fouraker, Cracker Barrel's Vice President of Strategic Sourcing.
"We recognize that gestation crates may not be the best method to meet higher animal welfare goals and are committed to evolving to sustainable alternatives."
The Humane Society of the United States ardently supports Cracker Barrel's move. "We are grateful to Cracker Barrel for putting a stake in the ground when it comes to the extreme confinement of sows in gestation crates," said Wayne Pacelle, president and CEO of The HSUS.
"Americans care about how farm animals are treated, and gestation crate confinement is simply out-of-step with those values."
Cracker Barrel acknowledges the importance of studies that have investigated consumer preferences regarding breeding pigs being housed in groups rather than individual crates, as well as the economic analysis reflected in the study by Iowa State University, documenting lower production costs for pork suppliers who use group housing.
Cracker Barrel is following other leading food companies — McDonald's, Burger King, Wendy's, Denny's, Kroger and Safeway — that have announced moves toward gestation crate-free supply chains.
"Compass Group, the nation's largest foodservice company with more than 10,000 dining operations across the country, enacted a policy to have its supply chain be gestation crate-free by 2017."
Major pork producers like Smithfield Foods and Hormel have also agreed to stop using gestation crates at their company-owned breeding operations by 2017, and Cargill is already 50-percent gestation crate-free.
"Cracker Barrel hopes that all of our pork suppliers will share in our vision for a gestation crate-free future, and we're excited to work with them to achieve this goal," added Fouraker.
In contrast, Domino's Pizza shareholders recently rejected a Humane Society proposal to even just study ending the use of pork from suppliers who confine pregnant pigs in small gestation stalls.
In response to Domino's decision, Texas Farm Bureau publications director, Mike Barnett, posted a letter on the bureau blog praising Domino's decision to oppose animal rights groups and not caving into HSUS demands.
Barnett closed his blog by thanking Domino's "for not playing follow-the-leader in these fast-food follies" and ordering an extra-large pepperoni pizza.
Barnett can whine like a sow till the cows come home, but the tide has turned in favor of consumers and activists who have a healthy regard for the welfare of living creatures on factory farms living on Death Row, waiting to be slaughtered.


text:
Canaveral National Seashore is located along Florida's Central East Coast. The
park is situated on a barrier island. The park is 24 miles long and is 57,000
acres of ocean, beach, dune, lagoon, and pine flatlands
Image ID: line1773, NOAA's America's Coastlines Collection
Location: Florida, Canaveral National Seashore
Photographer: Courtesy of NPS - Canaveral National Seashore
Category: Coastline/Southeast Florida/
• High Resolution Photo Available
of the U.S. Department of Commerce, National Oceanic & Atmospheric
Adminstration (NOAA),
NOAA Central Library
NOAA Privacy Policy
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Last Updated:
June 10, 2016


text:
- Professor of Creative Writing
- Bachelor of Arts, University of Hawaii at Manoa, 1980
- Master of Arts, SUNY Binghamton, 1985
Sylvia Watanabe's short story cycle, Talking to the Dead, was a finalist for the 1993 PEN Faulkner Award. She is the recipient of an NEA fellowship, a Josephine Miles Pen Oakland award in fiction and an Ohio Arts Council grant in nonfiction. Watanabe's fiction and nonfiction have appeared in many anthologies, including the O'Henry Prize and Pushcart Prize collections. During the 90's she co-edited two volumes of Asian American fiction, Home to Stay and Into the Fire, with Carol Bruchac, of the Greenfield Review Press. Home to Stay was the first anthology of Asian American women's fiction to be published in the U.S. In 2007-08, she received an Oberlin College award for excellence in teaching. Her current book-length fiction project is about the survivors of the American nuclear testing in the Pacific.


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Or Login using BecomeGorgeous
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Published on: 25 Jun 2013 in Dark Hair Color Ideas 2013
Balayage hair highlights by Anne Brightmore Artistic Team.


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A Brow Lift (also known as a Forehead Lift or an Upper Face Lift) is a surgical procedure designed to lift the brow and eliminate wrinkles on the forehead. People with droopy or sagging brows can have a sad, angry, or tired appearance; a brow lift can make that person look more happy and energetic.
The most common signs of a patient who would benefit from a brow lift are:
If you feel that your face does not reflect how happy, young, and energetic you actually feel, then you may be a candidate for a brow lift.
The best way to find out if a brow lift is right for you is to consult with our doctors. Our expert surgeons will examine your skin, including its texture and elasticity, and evaluate the tone of your underlying facial tissues and bone structure. Dr. Nichter or Dr. Horowitz will also consider the aesthetics of your eyes and how a brow lift would affect their appearance.
A brow lift can also adjust the position of your hair line, lowering it if it is too high or raising it if it is too low.
If a patient's primary concern is the "frown" or "worry" lines between the eyebrows and at the top of the nose, a limited brow lift to address only this problem may be possible.
Brow lifts are very versatile procedures which can be performed a number of ways to varying degrees. The outside (lateral) brow can be raised or the entire brow, depending on what is required. This is why it is so important to select a highly qualified surgeon to match your needs and goals with the right procedure.
Brow lifts are done on an outpatient basis. They can be performed under general anesthesia or under local anesthesia with intravenous sedation.
The specific procedure your brow lift entails will depend on your needs. Factors such as whether entire brow or only the outer brow is to be raised, repositioning of the hairline, and the patient's individual facial contours will determine the placement and extent of the incisions.
By using a beveled incision technique, our surgeons preserve as many of your hair follicles as possible. This means that individual hairs will grow into the scar area, often hiding it to the extent that you could wear your hair pulled back.
Dr. Horowitz and Dr. Nichter also perform endoscopic brow lifts, which make use of an endoscope: a narrow, hollow scope with a fiber-optic light source and a camera. Endoscopic brow lifts involve several short incisions behind the hair line.
Our office will provide you with complete information well ahead of your surgery date. This information is also available in our Patient Information section. Our doctors will go over with you which medications you should not take before your surgery, and they will advise you to stop smoking two weeks before your surgery.
Brow lift patients should expect to leave the surgery center with taping or light dressing, depending on the surgeon's preference. This will be removed during the first week following surgery. Bruising and swelling are normal at this point, and temporary asymmetry is very common. Most swelling will go away within 48 hours and bruising will resolve within one to two weeks. After one week, concealing makeup is permitted.
The result of your brow lift should become apparent after several weeks, and minor changes may occur over several months as your face settles in to its new appearance.
The brow lift is a relatively simple procedure and complications resulting from it are very rare. But like any surgery, it does carry some risks. Our surgeons will go over the risks with you and give you thorough instructions to avoid them.
The cost of a brow lift can vary quite a lot because brow lifts themselves can be done in a variety of ways. The factors which contribute to the cost of a brow lift are:
To speak to one of our board-certified plastic surgeons in Newport Beach about a brow lift, the costs, and other questions, call our office today.
Contact (949) 565-2121 to reach our office.


text:
I had ulthera last week and was wondering how long I should wait to get sculptra injection? Is it better to wait 1-2 months or can I have it done soon.
I Had Ulthera a Week Ago. How Long Should I Wait Before I Get Sculptra?
Doctor Answers 3
Interaction Between Ulthera and Sculptra
When Sculptra is injected properly in the deeper tissue planes below the skin, it would be unlikely that the ultrasound treatments with Ulthera would influence it's effect. Sculptra causes new collagen to form which is not apparent for several months and then slowly degrades over time. Sculptra is not a filler but a stimulator of your own collagen which then acts to create volume in a gradual, progressive, and predictable manner. Remember that little to no result is usually seen after the first treatment and the final result takes many months to appear.
Learn more: http://www.dorsetstreetdermatology.com/cosmetic-dermatology/sculptra/
Sculptra after Ulthera
It doesn't seem that there needs to be any delay after Ulthera before Sculptra can be done. ON the other hand, there may be good reason to delay Ulthera if it is to be done after Sculptra as the effect of the ultrasound energy on the Sculptra, if it reaches its depth, is unknown. However, once the Ulthera is done, the body responds by tightening the preexisting collagen and this should not damage the Sculptra that would be placed thereafter. I am not aware of any scientifically -controlled study that has examined this question.
Learn more: http://www.thenyac.com/sculptra/index.html
Sculptra Injections after Ulthera Treatment
Waiting one month generally should be a sufficient waiting period.
Sculptra Aesthetic are quick office injections performed in the office via local anesthesia. Sculptra stimulates the face to build collagen. Results are NOT immediate, rather gradually develop over time. Treatments are repeated approximately every 2 months, as needed, until a desired result is achieved. Once achieved, touch-up treatments may be need only every 1-2 years. Sculptra can only be placed in certain areas of the face, such as the cheeks. As with all the plastic surgery procedures, everything has its advantages and disadvantages.
Speak with your Sculptra injector. Only after a comprehensive evaluation can he/she help determine appropriate options for you.
Dr. Chaboki
Learn more: http://www.potomacplasticsurgery.com/med-spa/non-surgical-facelift.cfm
You might also like...
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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1Department of Cell and Developmental Biology, Vanderbilt University
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This protocol describes the isolation, enrichment, and maintenance of medulloblastoma tumor stem cells derived from mutant mice with ectopic Sonic hedgehog pathway activity.
Date Published: 9/01/2010, Issue 43; doi: 10.3791/2086
Keywords: Medicine, Issue 43, medulloblastoma, stem cells, isolation, in vitro culture
Huang, X., Ketova, T., LItingtung, Y., Chiang, C. Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells. J. Vis. Exp. (43), e2086, doi:10.3791/2086 (2010).
Brain tumors have been suggested to possess a small population of stem cells that are the root cause of tumorigenesis. Neurosphere assays have been generally adopted to study the nature of neural stem cells, including those derived from normal and tumorous tissues. However, appreciable amounts of differentiation and cell death are common in cultured neurospheres likely due to sub-optimal condition such as accessibility of all cells within sphere aggregates to culture medium.
Medulloblastoma, the most common pediatric CNS tumor, is characterized by its rapid progression and tendency to spread along the entire brain-spinal axis with dismal clinical outcome. Medulloblastoma is a neuroepithelial tumor of the cerebellum, accounting for 20% and 40% of intracranial and posterior fossa tumor in childhood, respectively1. It is now well established that Shh signaling stimulates proliferation of cerebellar granule neuron precursors (CGNPs) during cerebellar development 2-4. Numerous studies using mouse models, in which the Shh pathway is constitutively activated, have linked Shh signaling with medulloblastoma 5-9.
A recent report has shown that a subset of medulloblastoma cells derived from Patched1LacZ/+ mice are cancer stem cells, which are capable of initiating and propogating tumors 10. Here we describe an efficient method to isolate, enrich and maintain tumor stem cells derived from several mouse models of medulloblastoma, with constitutively activated Shh pathway due to a mutation in Smoothened (11, hereon referred as SmoM2), a GPCR that is critical for Shh pathway activation. In every isolated medulloblastoma tissue, we were able to establish numerous highly proliferative colonies. These cells robustly expressed several neural stem cell markers such as Nestin and Sox2, can undergo serial passages (greater than 20) and were clonogenic. While these cultured tumor stem cells were relatively small, often bipoar with high nuclear to cytoplasmic ratio when cultured under conditions favoring stem cell growth, they dramatically altered their morphology, extended multiple cellular processes, flattened and withdrew from the cell cycle upon switching to a cell culture medium supplemented with 10% fetal bovine serum. More importantly, these tumor stem cells differentiated into Tuj1+ or NeuN+ neurons, GFAP+ astrocytes and CNPase+ oligodendrocytes, thus highlighting their multi-potency. Furthermore, these cells were capable of propagating secondary medulloblastomas when orthotopically transplanted into host mice.
1. Micro-dissection of Tumor-bearing Cerebellum, Dissociation of Tumor Tissue and Plating
Note: Coat culture dishes with 0.1% gelatin for at least 30 minutes. Prepare fresh neural stem cell culture medium consisting of Neurobasal medium with glutamine, Pen-Strep, N2, B27, human EGF (25 ng/ mL) and basic FGF (25ng/ mL).
2. Enrichment, Maintenance and Expansion of Medulloblastoma Tumor Cells by Serial Passages
We usually get many colonies after 1 week of initial plating (Figure 1). These colonies can be dissociated and replated onto new gelatinized dishes for enrichment of the tumor cell population. First change to new culture medium, then simply use a 1-mL Pipetman to mechanically detach colonies followed by repetitive pipeting for 4 minutes to yield a cellular suspension (no Accutase needed). Check under a microscope to ensure that large cell clumps have been dissociated. Then dispense cell suspension onto new gelatinized dishes for further culturing and go through the same procedure for additional passages. Unattached tumor cells, blood cells and other cell types are removed by serial re-platings, leaving the attached tumor cells to expand rapidly. Change culture medium on the second day following initial seeding, and every other day thereafter.
3. Immunofluorescent Staining and Examination of Cultured Cells
1. Grow tumor cells on glass coverslips
On day 1, place one glass coverslip in each of a 6-well plate, then add 0.1% gelatin for 30 minutes at 37°C. Seed approximately 2-4X 105 tumor cells per mL. The cells should attach overnight and the neural stem cell medium changed on day 3. Staining is performed on day 4.
2 Immunofluorescent staining
Remove culture medium and wash cells twice with ice-cold PBS. Fix the cells with freshly-made 4%PFA for 15 minutes. Briefly wash cells twice with PBS and permeablize with 0.3% Triton (in PBS) for 5 minutes. Briefly wash cells twice with PBS and block with 10% goat serum for 40 minutes. Incubate the cells with primary antibody for 90 minutes, then wash cells three times with PBS, for 5 minutes each. Incubate the cells with secondary antibody for 60 minutes, then wash three times with PBS, 5 minutes each. Mount coverslips onto slides with 5 mL of an aqueous mounting medium and perform confocal microscopy.
4. Differentiation of Tumor Cells
Remove neural stem cell medium and briefly wash cells with PBS twice. Add differentiation medium on day 1, change medium on day 4 and determine level of differentiation by immunofluorescence staining on day 7. The differentiation medium consists of DMEM, Pen-Strep and 10% fetal bovine serum.
5. Representative Results
Morphology results
After Accutase treatment and gentle repetitive pipeting, the tumor tissue should be mostly dissociated into small cellular aggregates and single cells. Many sizable colonies can be observed as early as 5 days after initial plating, as shown by Figure 1. Highly proliferative tumor cells are often bi-polar with high nuclear/cytoplasmic ratio. These cells are seen typically radiating from small cellular aggregates attached to the gelatinized surface. Blood cells, small and round, are usually removed by subsequent media changes and serial platings. After several passages, one can observe uniformly distributed, proliferative Ki67+ tumor cells and little contamination with other cell types (Figure 2).
Expression of neural stem cell markers, clonal analysis and differentiation into multiple lineages
To determine whether the proliferative cells derived from dissociated cerebellar tumor tissue show characteristics of tumor stem cells, we performed further characterization by stem cell marker expression, clonal analysis and differentiation potency. We found that these isolated tumor cells highly express neural stem cell markers such as Sox2 and Nestin (Figure 3). As we retrieved tissues from SmoM2-YFP tumor, all tumor cells express YFP. Consistent with a recent study reporting that primary cilia are important for the development of Shh pathway-dependent medulloblastoma 12, SmoM2-YFP expression was clearly localized to the cilia of Sox2+ tumor cells (Figure 3). When plated at clonal density of 300 cells per mL of culture medium, we observed clonal expansion from a single tumor cell to a sizable colony (Figure 4). Furthermore, these cells were able to differentiate into various neuronal and glial cell types when cultured under pro-differentiation conditions (Figure 5).
Figure 1. Morphology of a highly proliferative colony after first plating of dissociated medulloblastoma tissue. Generally, a sizable colony forms within 5 days after initial seeding of dissociated tumor tissue, and a representative one is illustrated here in bright field. Bi-polar, elongated cells radiate from a dense core cell aggregate. Attached to the tumor cells are small, round red blood cells, which are gradually depleted by serial platings.
Figure 2. Morphology of established medulloblastoma cells beyond three passages. This bright field image shows the typical appearance of established medulloblastoma cells after several passages. The cells remain mostly bi-polar with high nuclear/cytoplasmic ratio. We show acetylated tubulin staining of tumor cells, most of which are cycling as shown by strong Ki67 expression.
Figure 3. Established medulloblastoma cells express neural stem cell markers. SmoM2-YFP marks cells expressing constitutively active Smo, hence Shh pathway activity. All cells of the established medulloblastoma cell lines expressed YFP, and most of them co-expressed high levels of various neural stem cell markers, such as Nestin and Sox2. Interestingly, when we performed YFP signal detection with minimal laser power during confocal microscopy, we detected concentrated YFP signal in the cilia of Sox2+ cells. This observation is consistent with a recent report 12 that described the essential role of cilia in the development of Shh pathway-dependent medulloblastoma.
Figure 4. Established tumor cells undergo clonal expansion. After dissociation into single cell suspension, tumor cells were plated onto a 24 well-plate at a clonal density of 300 cells per mL of culture medium. In each well we observed clonally expanded colonies. This series of bright field images demonstrate typical changes over a 10-day period of culture.
Figure 5. Differentiation analyses of established medulloblastoma cells. When tumor cells were switched from EGF/bFGF containing serum-free medium to DMEM/10% FBS, YFP+ tumor cells significantly altered their morphology and differentiated into various cell types, including Tuj1+ or NeuN+ neurons, GFAP+ astroglial cells or CNPase+ oligodendrocytes.
Subscription Required. Please recommend JoVE to your librarian.
We describe an efficient way to isolate, enrich and maintain medulloblastoma stem cells from primary tumor tissue retrieved from mutant mice with constitutively active Hedgehog signaling. We found that one critical step in successfully establishing a healthy tumor stem cell line is the Accutase treatment used during dissociation of the primary tumor tissue. In our experience, when first dissociating tumor tissue from the cerebellum, 4 minutes of 50% Accutase treatment at 37°C, followed by 3 minutes of repetitive pipeting using a 1-mL Pipetman will generally result in successful first seeding. It is also important to wait until sizable colonies are formed before re-plating (Figure 1). After the first seeding, Accutase treatment is no longer necessary; repetitive pipeting for 3-4 minutes using a 1-mL Pipetman is sufficient to dislodge and dissociate cell clumps for re-plating. Do not use smaller volume Pipetman as the finer tips will damage the cells extensively. An established cell line can be generated from each tumor tissue isolated and these cells are highly proliferative, robustly express several neural stem cell markers and can differentiate into both neuronal and glial cell types. We have performed orthotopic transplantation of these cells into immuno-compromised recipient mice which subsequently developed secondary medulloblastomas. These tumor cells in culture rarely exhibited spontaneous differentiation or apoptosis, features that are common to neurosphere culture methods. This protocol is designed for the successful propagation of tumor stem cells derived from primary medulloblastomas and thus, will enable us to test the effects of candidate genes and chemical inhibitors on Hedgehog-driven tumor cell growth and behavior.
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No conflicts of interest declared.
This study was supported by grants from the Vanderbilt-Ingram Cancer Center Support Grant (P30 CA068485), the Childhood Brain Tumor Foundation and the National Institutes of Health (NS042205).
|Catalog Number
|Neurobasal medium
|Primaria dishes
|Fisher Scientific
|Sox2 antibody
|EMD Millipore
|Mouse, 1:1000
|Nestin antibody
|Mouse, 1:200
|YFP antibody
|Molecular Probes, Life Technologies
|Rabbit, 1:2000
|GFAP antibody
|Chicken, 1:1000
|Tuj1 antibody
|Mouse, 1:2000
|NeuN antibody
|EMD Millipore
|Mouse, 1:2000
|CNPase antibody
|EMD Millipore
|Mouse, 1:1000
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Doucette Duvall Bonnie in Christmas
Another fabulous dress by the design team Doucette Duvall. This adorable wool plaid dress is perfect for work or play. Short sleeve with A-line shaping and a cute front pocket. Back zip and fully lined. Fits true to size. Perfect for work and after!
Tags: ruffles, cocktail dress, Love it, holiday, Cynthia Rowley, black taffetaAdded 9 Years Ago from Rare Chic
Tags: designer, cocktail dress, pockets, Love it, holiday, polka dot, black dress, LBD, one-shoulderAdded 9 Years Ago from Rare Chic
I love this dress for holiday parties!Tags: Love it, holiday, silk dress, Geren Ford, grey dress, silver dress, rouchingAdded 10 Years Ago from Rare Chic


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Fort Lauderdale, Fla. (UPI) Oct 7, 2010
Higher than normal tides driven by the forces of the moon and high winds are hitting South Florida's coast, along with dangerous rip currents, officials say.
Water levels are rising along seawalls, docks and canal banks this week as the tides reach their fall peak, the South Florida Sun-Sentinel reported Thursday. Ankle-deep water flowed over a section of a Fort Lauderdale boulevard and an inch of water covered parts of a state highway near Hollywood, the newspaper said.
Low-lying areas were expected to be vulnerable to similar soakings as high tides will persist through Friday, forecasters said.
Stiff onshore breezes, coupled with the gravitational pull from a new moon, are the culprits, they say.
"We have a lot of wind coming down from the north, northerly winds about 20 to 25 mph," meteorologist Chuck Caracozza of the National Weather Service said.
"It's a normal event; it happens every year," said Nancy Gassman, natural resources administrator for Broward County, noting this week's tides are 8 to 10 inches above average. "The fall tides tend to be more extreme."
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Otto Penzler
Photo: © Carolyn Hartman
About the Author
Otto Penzler is the proprietor of The Mysterious Bookshop in New York City. He was publisher of The Armchair Detective, the founder of the Mysterious Press and the Armchair Detective Library, and created the publishing firm Otto Penzler Books. He has twice received the Edgar Award, for The Encyclopedia of Mystery and Detection and The Lineup: The World's Greatest Crime Writers Tell the Inside Story of Their Greatest Detectives, as well as the Ellery Queen Award from the Mystery Writers of America for his many contributions to the field. He is the editor of The Black Lizard Big Book of Pulps, which was a New York Times bestseller, and several more Vintage Crime/Black Lizard anthologies, including The Big Book of Black Mask Stories, The Big Book of Locked-Room Mysteries, The Big Book of Sherlock Holmes Stories, and The Big Book of Jack the Ripper. He is also the series editor of The Best American Mystery Stories of the Year. His other anthologies include Murder for Love, Murder for Revenge, Murder and Obsession, The 50 Greatest Mysteries of All Time, and The Best American Mystery Stories of the Century. He wrote 101 Greatest Movies of Mystery & Suspense. He lives in New York City.


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The CIM really prepared me for everything I need to know.
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Prepare for achievement and advancement.
Start with an education that opens more doors.
Successful businesses need people with well-developed business skills–people who have the knowledge to hit the ground running, and those who are committed to taking their business to the next level. Having an undergraduate or graduate business degree on your resume helps increase your opportunities for employment, advancement, job security, and higher earnings over your lifetime.
An undergraduate business degree can help you get a better job with strong demand for business professionals at every level. In fact, the National Association of Colleges and Employers Job Outlook 2010 reports that business-degree-holders are among the most in-demand graduates in the U.S.
Adding a graduate degree to your credentials demonstrates commitment and drive to current and potential employers. Baker College Center for Graduate Studies programs have been developed specifically for working adults who seek a real-world focus and practical application that is rare in graduate-level education.
Baker College undergraduate and graduate programs concentrate exclusively on career areas with the highest demand. We have one the highest graduate employment rates in the country, giving you the best chance for a successful career.
Bring Your Own Device Initiative
Beginning winter 2015, all students enrolled in an undergraduate business program* at Baker College are required to bring your own (portable/mobile) device to class. These devices are available at your campus bookstore. We have worked diligently to choose quality products at affordable prices. If you already own a device you are not required to purchase another.
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Baker Center for Graduate Studies programs are designed to provide you with the knowledge, practical skills, and career tools you'll need to succeed in today's professional environment. Their flexible structure accommodates the schedules and responsibilities of busy working adults.
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The Hypotensive Effects of Oregano Oil's Main Component
A recent study shows hypotensive action of Carvacrol, the most abunndant molecule in Oregano essential oil:
Hypotensive Effects of Carvacrol on the Blood Pressure of Normotensive Rats.
Aydin Y, Kutlay O, Ari S, Duman S, Uzuner K, Aydin S.
Eski?ehir Osmangazi University, Faculty of Medicine, Department of Physiology, Eskisehir, Turkey.
Carvacrol is the major compound of essential oils of many plants, ethnomedically used for centuries but there were no detailed investigations on its action on the cardiovascular system. The aim of our study was to investigate the role of carvacrol on the cardiovascular functions of anesthetized rats and IN VITRO of isolated rat aorta. Carvacrol (100 mug/kg, I. P.) decreased heart rate, mean arterial pressure and systolic and diastolic blood pressures of the anesthetized rats whereas there were no effects at 1, 10 and 20 mug/kg. Carvacrol was observed to exhibit hypotension and to inhibit N((omega))-nitro- L-arginine methyl ester ( L-NAME)-induced hypertension. The lack of inhibitory action of carvacrol (10 (-4) M) on the CaCl (2)- and phenylephrine-induced contractions of isolated rat aorta showed that neither adrenergic receptors nor voltage-dependent vascular L-type calcium channels were involved. But, based on previous investigations, the involvement of cardiac L-type calcium channel blocking actions are suggested for the hypotensive actions of carvacrol was assumed.
*The FDA has not evaluated the statements on this website. The information presented here is for educational purposes of traditional uses and is not intended to diagnose, treat, cure, or prevent any diseases.


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Louis Prima Biography
7 December 1911, New Orleans, Louisiana, USA, d. 24 August 1978, New Orleans, Louisiana, USA. A trumpeter, band leader, singer and composer, Prima was the son of Italian immigrant parents. He was educated at Jesuit High School, and studied the violin for several years under Hemmersback, before switching to the trumpet. At the age of 17, inspired by jazz greats such as Louis Armstrong and King Oliver, he gained his first job as a singer/trumpeter in a New Orleans theatre - his elder brother, Leon, also played trumpet at a local nightspot. For a time in the early 30s Prima worked with Red Nichols, before forming his own seven-piece New Orleans Gang, with its signature tune, Way Down Yonder In New Orleans, who recorded more than 70 titles in New York for various labels from 1934-39. Several of them made the US Hit Parade, including The Lady In Red, In A Little Gypsy Tea Room and The Goose Hangs High. His sidemen during this period included Georg Brunis (trombone), Claude Thornhill (piano), George Van Eps (guitar), Artie Shapiro (bass), Eddie Miller (reeds), Ray Bauduc (drums), Sidney Arodin (clarinet), Frank Pinero (piano), Frank Frederico (guitar), Oscar Bradley (drums), and Pee Wee Russell (clarinet). By this stage, Prima was also composing songs, and one of them, Sing, Sing, Sing, when developed by Benny Goodman, became a smash hit for the King Of Swing, and remains a Swing Era classic.
Over the years, Prima wrote or co-wrote many other numbers, including Robin Hood, which was successful for Les Brown in 1945, and the 1947 Jo Stafford hit, A Sunday Kind Of Love, along with Alone, Little Boy Blew His Top, Marguerita, New Aulins, Angelina, Where Have We Met Before?, Brooklyn Boogie, Boogie In The Bronx, Bridget OBrien, Boogie In Chicago, Its The Rhythm In Me, Sing A Spell, Its A Southern Holiday and Rhythm On The Radio. His collaborators included Jack Loman, Dave Franklin, Milton Kabak, Bob Miketta, Barbara Belle, Anita Leonard, and Stan Rhodes. After making an good impression on his feature film debut in the Bing Crosby movie musical Rhythm On The Range (1936), Prima continued to have relatively small, but telling roles in a number of other movies, notably Rose Of Washington Square (1939), in which he enhanced Alice Fayes rendering of Im Just Wild About Harry with his ebullient and exciting trumpet accompaniment. By this time he had his own big band which he fronted with great showmanship and panache. It had 40s hits with Angelina, Bell-Bottom Trousers (vocal: Lily Ann Carol), and Civilization (Bongo, Bongo, Bongo), an amusing novelty from the 1947 Broadway revue Angel In The Wings.
In 1948, Prima began working with the poker-faced singer Keely Smith, and, after having a US hit in 1950 with their joint composition, Oh, Babe!, they were married two years later. During the next decade they were recognized as one of the hottest nightclub acts in the USA, and became known as The Wildest Show In Las Vegas. Primas inspired clowning and zany vocals delivered in a fractured Italian dialect, coupled with Smiths cool image and classy singing, were augmented by tenor saxophonist Sam Butera and his group, the Witnesses. A typical performance was filmed at Lake Tahoe in 1957, and released under the title of The Wildest, and they reassembled in 1959 for the featureHey Boy! Hey Girl! Prima and Smith were awarded Grammys in 1958 for their inimitable reading of the Harold Arlen - Johnny Mercer standard, That Old Black Magic.
In 1958 Prima was briefly in the UK Top 30 with Carl Sigman and Peter de Roses likeable Buona Sera, and two years later made the US singles and albums charts with the instrumental Wonderland By Night. Other Top 40 albums includedLas Vegas-Prima Style and Hey Boy!, Hey Girl! In 1961, while still at the height of their fame - and having recently signed a multi-million dollar contract with the Desert Inn, Las Vegas - the couple were divorced. Prima and Butera subsequently attempted to cash in on the then-popular dance fad by appearing in the movieTwist All Night, which sank without a trace, in spite (or because) of items such as When The Saints Go Twistin In. Far more lasting was Primas contribution in 1967 to T he Jungle Book, the Walt Disney Studios first cartoon feature for four years, which went on to gross around $26 million. Prima provided the voice of hip orang-utan King Louie, and sang the films hit song, I Wanna Be Like You. In later years he mostly confined himself to performing with a small group at venues such as the Sands Hotel, Las Vegas, and in 1975 underwent surgery for the removal of a brain tumour. He never recovered from the operation, and remained in a coma until his death nearly three years later in a New Orleans nursing home.
Source: The Encyclopedia of Popular Music by Colin Larkin. Licensed from Muze.


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I was doing some research on short-term missions when I found a blog by Vinoth Ramachandra, a Christian writer in Sri Lanka. He has studied and traveled in Europe, done extensive ministry in South Asia, and he has written cogent criticism of Christianity as it is received in the non-Western world. He clearly and accurately writes things that the West needs to hear, both praising the good and condemning the bad. Incisively addressing everything from the War on Terror, whistleblowers in the US government, and US foreign policy to relations between Western and Eastern Christians, missionary work done badly, and the influence of media on relations in the Church worldwide, Ramachandra is an intelligent voice from the "other side" of world Christianity.
Right now I want to focus on some of the things that Ramachandra has to say about partnership between richer evangelical churches and the Church in the developing world, particularly in the areas of short-term missions and development and provision of educational resources. Because Ramachandra is a Christian in a country where Christians are a minority population, he has a unique perspective on what the various parts of the Church have to offer to one another.
On the subject of short-term missions, he is able to speak from the part of the world that receives short-term trips. In his post "Who Says 'No' to 'Mission Trips'?" from May 7, 2010, he talks about the difficulties that short-term mission trips foist upon churches in the developing world. He details the imbalanced "partnership" between rich STM-sending churches and the poorer churches receiving them, demonstrating that STMs are often unnecessary, unhelpful, and unhealthy for the receiving churches. In the next post, he further clarifies that STMs, as they are done in evangelical circles, go away from dying to self and following Christ's example in the Incarnation as he took on our sin and suffering. Speaking from the "other side", he intelligently shows how STMs fail to serve the poor on the one hand and fail to serve the spiritual formation of the short-term missionaries on the other hand.
On the subject of educational resources, he talks about the influence of Western media and publishing companies in the education of the Church. In "Authentic Partnerships" from October 1, 2010, Ramachandra describes the pressure that Western literature exerts on the Church around the world. Christian literature from richer countries floods churches in poorer countries that have decent theologians, but Western publishers do not take up their writings. The poorer side winds up having to fit the richer side's agenda. In "Reformed Amnesia?" from March 28, 2013, he even challenges the Gospel Coalition on their mission to provide resources to Christians around the world: he inquires just who it is who gets to decide what the rest of the world needs and what counts as good doctrine. Unless the West just wants to export their sectarian divides and theological controversies, he urges the West to receive from the "other side" of world Christianity, and he even holds up the Roman Catholic Church as a Christian tradition that has listened well to the rest of the churches in its communion. Achieving a Church-wide theological consensus is a must, not just teaching everyone what was decided in North America.
Theology from the global Church is not just a subset of theological foreign policy. Ramachandra outlines issues between the West and the rest of the Church that need serious consideration and careful action to resolve. While I would be punching above my weight to demand an ecumenical council to reach a theological consensus and sort out problems of Church discipline, I believe that we in the West could start by carefully studying and accepting what the likes of Vinoth Ramachandra have to say.


text: Darkwaknight November 23, 2009 2:01 PM Last Edited 541 days ago Wow by the sounds of some on here I am surprised Yamaha or Wsbk as a whole do not just fold now Ben Spies has gone!!!??!! The Guy is a talent no doubt and had a great, super year but had he had JT,Bayliss and Haga (more so Bayliss) to deal with last year he would not have won as many races or Poles (and possible the WC) but if,buts and maybe's mean little now. Superbikes was great Before,during BS and will be just fine without him. Lets hope we get a great year of racing as I hold less and less hope for that in GP's!! Reply Report abuse 22 agree 9 disagree


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LYON, France (May. 22)
Representatives of six Jewish, Catholic, and Protestant welfare organizations met here today and mapped plans for coping with the influx of refugees from Algeria to this area.
Stressing that the conference had "no political purposes whatever," the meeting resolved to call the attention of the public authorities to "certain social and economic problems posed by the Algerian drama."
The conference elected three co-chairmen. They are Cardinal Gerlier, representing the Catholic group; Pastor Arger, of the Protestant community; and Lyon Chief Rabbi Klink. The conference noted that "social work must be implemented on behalf of the Algerian repatriates, whose numbers are growing daily."