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found my FREEDOM: Carnival Freedom Cruise Review by chill636
Overall Member Rating
found my FREEDOM
Destination: Western Caribbean
Embarkation: Fort Lauderdale (Port Everglades)
The driver (named Luiz)still came to the airport to pick us up which was very nice of him. The dEcor of the Hyatt Place was very clean and modernly designed. Large flat screen TVs in every room, pull out couch and plush mattress padding made for a wonderful night's sleep after a long day of traveling. There is an outside pool, the fitness center is better than expected and so was the free continental breakfast buffet. There was fresh fruit salad, fresh juices, milk, variety of cereals, yogurt, bagels and toast. You can also have breakfast made to order More for purchase. Day 1 embarkation- instead of arriving at the cruise terminal at 10am as planned; DH and I took the shuttle back to the airport at 12 to pick up my luggage. We arrived at the port approx 1:40pm and surprisingly the boarding process was smooth. There was a long line that went very quickly, we were on the boat in 30min! The FREEDOM's dEcor was just as described as fellow cc'ers noted; very dark and sharp that can appear to be cluttered and out of sync BUT there is a method to the madness. Each part of the ship is supposed to give the feel of a certain time period in history ranging from Babylonian times to modern times.
Tip: it is no longer required to wear the life vest for the boat drill...the wearing of the vest is now OPTIONAL.
Day 2 was a sea day and also formal night. Take advantage of the day and the activities available. The Adult Serenity is exactly as described; a place for comfort and serenity. Definitely a great place to hide from the crowd.
Day 3 Cozumel, Mexico: The fam and I went to Paradise Beach and had a blast! There is no entry fee. but $2 for the use of chairs and umbrellas (which is a great price) and $12 allows you use of the water activities i.e. mountain climbing, trampoline, slide, etc. It also allows you to kayak safely in the waters. DH and I were among the first to arrive at the beach and had to pay. By the afternoon when the crowds arrived they weren't charged a dime..just an extra tip. We enjoyed the beautiful emerald waters and even got a couple's massage for $50. There's a restaurant avail (we didn't use) and heard there were even free drinks we missed out on.
Day 4 was a sea day so I will not elongate the review by listing all the activities I enjoyed but I will say DH and I treated my sis and her hubby for a night at the Sun King (steakhouse). We thoroughly enjoyed it. Now let's just jump to day 5 COSTA RICA: based off reviews we decided to book a tour ahead of time with JJ Tours. Andres was our tour guide and he was awesome to say the least!! Spoke English very well, very informative and accommodating. But I will say he can move very slowly at times so keep track of time to encourage him to move along. Our 4 hour tour was 6 hours so please keep that in mind. We only had about 10 mins to shop before we heard the horn of the boat! Despite all of that I would still book with JJ Tours again if I had the chance. it was well worth the 45pp. The tour consisted of a boat cruise along the rain forest to see animals in their natural habitat, we tried to zip line but the cruise line gets first priority and it got very crowded so we opted for lunch at the beach, skipped the banana plantation and went back to the zipline. The zip line is an addt'l charge of 45 but still cheaper than other excursions including the zipline. My niece and nephew did the zipline and loved every moment of its adrenaline rush and optic zoom of the wildlife in the rainforest. Awesome is the only word to describe the entire day.
Day 5 LIMON, PANAMA: Warning: everything is cheap in Panama EXCEPT CABS!! It's hard to negotiate with them it will be cheaper to latch on with a group and take a tour but DH and I didn't want to take a tour we had very specific things we wanted to do. For $50 A PERSON we visited the Gatun Locks, panama canal, very beautiful beach with clear warm waters (Playa Langosta), and shopping in the Madison area, Great prices on everything imaginable. Our cab driver spoke very little English and we speak very little Spanish so that made the day very interesting...lol
Day 6 and 7 were sea days and the last formal night. Here are a couple of boat tips I missed:
Find the secret deck on decks 6, 7 and 9 definitely worth it The Fish and Chips spot is still a hidden gem even on this ship. The general masses still were unaware of its existence.
Sadly I didn't make it to the sushi bar BUT I noticed Carnival does something different since my last cruise..they have something called late night snacks every night starting at around 11:30 on deck 5, Very timely. Tip: you HAVE to attend karaoke nights, it is a MUST! There is an eccentric and eclectic gentleman named KIM that makes the evening entertaining, hilarious and LOTS of fun!!
Last but not least dining room tip: you MUST try the apple pie when its on the dessert menu which is ONE night! Definitely gives the warm chocolate melting cake a run for its money! (The diet apple pie tastes just as good).
Disembarkation was fun, fast and smooth. We opted to do the self-assist where you keep your bags with you and take them off the ship; very convenient. We pulled into port at 6:30 had a quick bite on the Lido and was in a cab heading back to the Hyatt Place South by 8am. Hope you enjoyed the review. Feel free to email me if there are any questions firstname.lastname@example.com Less
Published 08/12/10
Cabin review: 1329
very noisy floor, unruly kids, sink leak never fixed
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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
The increasing availability of semiconductor-based nanostructures with novel and unique properties has sparked widespread interest in their use in the field of biosensing. The precise control over the size, shape and composition of these nanostructures leads to the accurate control of their physico-chemical properties and overall behavior. Furthermore, modifications can be made to the nanostructures to better suit their integration with biological systems, leading to such interesting properties as enhanced aqueous solubility, biocompatibility or bio-recognition. In the present work, the most significant applications of semiconductor nanostructures in the field of optical biosensing will be reviewed. In particular, the use of quantum dots as fluorescent bioprobes, which is the most widely used application, will be discussed. In addition, the use of some other nanometric structures in the field of biosensing, including porous semiconductors and photonic crystals, will be presented.
The increasing availability of techniques for the fabrication and characterization of semiconductor-based nanometric structures with controlled composition and dimensions has sparked widespread interest aiming at their use in different biotechnological systems [
Furthermore, the typical size of semiconductor nanostructures, comparable to that of many common biomolecules, makes them to be appropriate for the development of hybrid systems [
The interest in the replacement of conventional molecular tags, such as fluorescent chromophores, with nanostructures resides in the superior physico-chemical properties of nanostructures compared to the molecular species they replace [
In the present work, the most significant applications of semiconductor nanostructures in the field of optical biosensing will be reviewed. In particular, the use of quantum dots as fluorescent bioprobes, which is the most widely used application, will be discussed. In addition, the use of some other nanometric structures in the field of biosensing, including nanoporous semiconductors and photonic crystals, will be discussed.
The most common method of detecting and quantifying biomolecules still remains the use of fluorescence [
However, many of the organic dye and protein-based fluorophores suffer from serious chemical and photophysical limitations caused by their intrinsic properties, which have limited their effectiveness in long-term stability and simultaneous detection of multiple fluorescent signals, i.e. multiplexing, without complex instrumentation and processing. Some drawbacks that can be highlighted include narrow absorption windows coupled to broad red-tailed emission spectra via small Stokes shifts, short excited state fluorescent lifetimes, pH dependence, self-quenching at high concentrations and susceptibility to photobleaching.
The unique fluorescent and overall optical properties of semiconductor quantum dots (QDs or semiconductor nanocrystals), make them very interesting fluorophores for both
Quantum dots usually show symmetric and narrow (bandwidth of around 30 to 50 nm full width at half maximum) photoluminescence spectra spamming the ultraviolet to near-infrared, thus enabling emission of pure color (
Furthermore, the possibility of tuning the emission from the QDs as to improve spectral overlap with a particular acceptor dye, make QDs suitable for their use as efficient fluorescence resonance energy transfer (FRET) donors. Moreover, QDs also emit light at a rate slow enough to eliminate most of the autofluorescence in the background but fast enough to maintain a high photon turnover rate. Therefore, they are ideal probes for timegated detection with enhanced selectivity and sensitivity. Additionally, it is possible to obtain polarized fluorescence by using shape-controlled QDs.
However, although possessing superb optical characteristics, QDs undergo an intermittent on–off emission under continuous excitation. This property, called blinking, is only partially understood and has been attributed to Auger ionization, caused by fluctuations in net charge inside or around the nanocrystals [
The fabrication techniques for the growth of semiconductor nanocrystals have been extensively developed in the past few decades, mainly focused on traditional applications in optoelectronic devices. These techniques allow the size, shape and composition of the nanocrystals to be varied over a remarkably wide range. Generally speaking, semiconductor QDs for applications in the field of optical biosensing are synthesized with mostly direct-band-gap materials (II–VI or III–V column elements of the periodic table). Accordingly, quantum dots made of ZnS, CdS, ZnSe, CdTe and PbSe, emitting from the UV to the infrared have been prepared for bioapplications, although these may need refinement as issues of reproducible synthesis and inorganic passivation remain to be optimized.
There are two major problems associated with the stability of luminescence from semiconductor nanocrystals. The first one is related to the presence of surface states arising from surface non-stoichiometry and unsaturated bonds. Crystalline imperfections and defects found on the surface of QDs will capture excited state energy and provide nonproductive and non-emissive pathways for deactivating the QD after they have been excited with light. Secondly, the surfaces of uncapped QDs tend to be very reactive due to their high surface-area-to-volume ratio, and consequently they become easily polluted by a variety of agents, which in turn provide additional trapping pathways that also result in quenched emissions. At the same time, uncapped QDs are so reactive that they are prone to spontaneous dissolution or photochemical degradation. Hence, even the simple act of diluting QDs often leads to irreversible decomposition of the nanocrystals. Therefore, control of the surface properties is mandatory for the formation of highly luminescent and chemically stable QDs. This issue has led to the development of core-shell quantum dot structures with improved properties [
Core-shell geometries where the nanocrystal is encased in a shell of a wider band gap semiconductor have resulted in increased fluorescence quantum efficiencies (over 50%) and greatly improved photochemical stability [
As a result of their superior optical properties QDs are being increasingly used for
The typical physical size of core-shell QDs is almost an order of magnitude larger than many of the conventional organic dyes in use (
Since highly luminescent QDs are usually fabricated from organometallic precursors and salts they have no intrinsic aqueous solubility, are often non-biocompatible, and do not have any reactive functional groups for conjugation with biomolecules [
In this regard, the typical size of the QDs, comparable to or slightly larger than that of many proteins (
Colloidal quantum dots with a wide range of bio-conjugation and with high quantum yields are now available commercially. The range of biological experiments that these materials are employed in is rapidly growing, being this one of the first commercial applications of modern nanotechnology. Specific binding to cell surfaces, insertion into cells, and binding to cell nuclei or other organelles have all been demonstrated following conjugation of the nanoparticle with the appropriate targeting protein. A range of biomolecules, including deoxyribonucleic acid (DNA) and proteins have been conjugated to QDs and used in diverse biomedical studies such as
Several aspects of biological relevance regarding the use of QDs emerge when a novel biomedical application is envisaged. These aspects include stability, toxicity and internalization mechanism, which are in many cases interrelated. All these potential sources of conflict can be well focused by appropriate surface biofunctionalization to obtain bioconjugates composed of QD nuclei with surrounding biomolecules based on amino-acid or nucleic-acid sequences. Furthermore, with an efficient surface binding of such molecules one can exploit the specific recognition properties of proteins and oligonucleotides opening the gate to preferential site organization of the QDs in the cell.
Cell viability studies are mandatory steps before application of any QD-complex [
Internalization of QDs in the cells can take place in diffusive and active forms [
Alternative methods for biofunctionalization exist based mainly in the interaction of the QDs with lipid layers, bilayers and nanomicelles conveniently linked to antibodies or other kind of proteins [
With such a large choice of biofunctionalization possibilities, QD cell internalization studies are currently merged in an intensive development. Studies include QD evaluation in both eukaryotic and prokaryotic [
Examples of the degree of development of applications by using internalized QD conjugates include
QDs are not meant to replace fluorescent dyes and proteins, but rather to be a specialized tool that can augment and complement them. However, several associated areas still need to be developed, principally the surface functionalization ligands and the related methods for conjugating QDs to biomolecules (especially orthogonal conjugation). As these mature, QDs are expected to become a more versatile tool for all aspects of fluorescent biosensing. In the following sections some particular examples of the use of quantum dots in the field of biosensing will be shown.
The unique advantages that QDs offer over conventional dyes has increasingly led to their use in immunoassay detection. For biosensing, the greatest potential of QD-antibody bioconjugates is in multiplexing [
Semiconductor nanocrystals can be used as to increase efficiency of photochemical reactions and can be effectively coupled to biomolecular units such as enzymes, to generate novel photoelectrochemical systems. Within this approach two broad strategies have been followed involving the use of the electrochemical properties of QDs for biosensing: (i) the monitoring of QD electrochemistry directly; or (ii) monitoring of QD photoelectrochemistry. As an example of the first approach, a multiple protein aptamer-based biosensing capability is coupled to the enormous amplification feature of nanoparticle based electrochemical stripping measurements to yield remarkably low detection limits [
One method for using QDs in biosensing is to create a donor/acceptor complex, which exhibits switching capability via fluorescence resonance energy transfer (FRET). FRET between donor and acceptor molecules has been extensively used in biophysical and biochemical studies to probe ligand-receptor binding and molecular structural changes. This is directly attributable to the sharp efficiency dependence of the process on the donor-acceptor separation distance at the 1–10 nanometer scale. QDs offer several advantages when used as FRET donors in place of organic dyes [
In this regard, several studies have demonstrated the effective use of QD FRET donors to detect small analytes by utilizing a common strategy that relies on conjugating QDs to target binding receptors which can be either proteins, antibody fragments or DNA aptamers. The QDs conjugates are then exposed to appropriate acceptor-labeled target analogs which are brought in close proximity to the QDs by binding to the receptors. In this initial state, the QD-donor photoluminiscence is quenched by efficient FRET to the proximal acceptor-labeled analogs. The presence of the target then displaces bound analogs from the surrounding conjugated receptors and this is detected through a reduction in FRET efficiency and the concomitant QD photoluminescence increase. This strategy has been utilized for detecting diverse analytes. Overall, this type of detection method benefits from a wide library of receptor proteins, antibodies and aptamers which provide both flexibility and specificity. However, it requires the presence of labeled analogs in solution, and is therefore not suitable for continuous monitoring. However, it is worth noting that some of the same optical properties that make QDs excellent FRET donors may also hinder their use as FRET acceptors. While their broad absorption spectrum and high excitation cross sections result in large spectral overlaps and high FRET efficiencies, this can also result in the unavoidable direct excitation of the QD acceptor at a rate that is often greater than the FRET induced excitation. In addition, the QDs longer exciton lifetime compared to that of many organic dyes may also hinder efficient FRET from dyes to QDs.
An interesting biosensor showing reversible FRET has been fabricated by connecting CdSe/ZnS core/shell QDs with a photoactivatable species that functions as the reversible FRET acceptor [
Additionally, the optical properties of QDs can be exploited as to improve the assay sensitivity of single particle DNA sensing. Incubation of dye-labeled DNA targets with biotinylated capture DNA probes allows their conjugation to streptavidin QDs only when the two DNA sequences hybridize. This hybridization is then detected via FRET between the QD and the dye acceptor. This approach utilizes commercial QD materials and their large size (∼30 nm diameter) initially suggested that relatively poor FRET efficiency would result. However, in this particular case, the high QD quantum yield (≥ 50%), the high Cy5 acceptor molecular extinction coefficient (∼250,000 M−1 cm−1) and a large number of acceptors (typically 12 to 54) around each donor can combine to overcome distance constraints arising from the QD size. Additionally, the background due to acceptor direct excitation is virtually eliminated through the choice of an appropriate excitation wavelength. This led to a 100-fold improvement in sensitivity compared to single organic dye molecular beacon-based detection. These type of sensing schemes can also be adapted for their use in a multiplex format. The narrow and symmetric QD emissions allowing easy spectral deconvolution and the most straightforward configuration relies on several QD populations interacting with the same dye acceptor, rather than the opposite.
Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots, such as zinc sulfide–capped cadmium selenide nanocrystals, into polymeric microbeads at precisely controlled ratios [
As such, these recent investigations certainly open up vast opportunities for creating a new generation of fluorescent markers with immense promise and potential in biological assay and detection. However, despite some breakthrough advances in the synthesis of QD-tagged latex beads and their applications to multiplexed optical encoding, there remain many problems yet unresolved. The main challenges that must be addressed in the use of these nanobeads in biological applications are how to better control the incorporation of the QDs into the polymer particles, as well as the control of colloid stability and monodispersity. More recently, work has also been done in the area of incorporating QDs into biocompatible polymer beads.
In addition to nanoparticles, novel materials such as porous silicon (PS) and porous carbon with nanometric pore size, compatible with the dimension of biomolecules have been used for biosensor applications. Other nanoporous thin films such as zinc oxide have also been used.
Since the discovery of its strong visible luminescence at room temperature, nanostructured porous silicon has attracted considerable interest aiming at the fabrication of optoelectronic devices, including biosensors. Additional interest is given to PS since this material can be fabricated easily using some of the established processes of the usual silicon technology, by partial electrochemical dissolution in hydrofluoric acid-based solutions, thus leading to controlled morphology at a nanometer scale in three dimensions. Furthermore, its large surface area allows quite easy chemical surface modification thus allowing the development of sensitive biosensors [
PS-based biosensors based on optical interferometry have been developed, allowing the detection of small organic molecules (biotin and digoxigenin), 16-nucleotide DNA oligomers and proteins (streptavidin and antibodies) at pico- and femtomolar analyte concentrations [
Furthermore, PS-based microcavities have been developed for their use as biosensors, by exploiting the luminescent properties of PS [
Finally, potentiometric biosensors based on porous silicon have been described [
One of the most common materials used as matrices for the design of biosensor systems is carbon. Graphite, glassy carbon and carbon paste are some of the carbon forms that have been used so far for biosensor development [
Glassy carbon is another material that has been well established for many years as an electrode material and more specifically as a biosensor immobilization matrix. Structurally, it is a compact solid, mechanically stable, impermeable to gases and fluids and chemically resistant over a broad range of situations. In addition, the high porosity, the low background current over a wide potential range and the great applicability of this material make it ideal for the adsorption of large molecules, while at the same time suggest its possible use as an electrochemical transducer. Recently, novel material processes allow for the formation of porous glassy carbon, leading to the formation of micro, meso or macro porous structures. One of the drawbacks of this material when used in biosensor design is its low sensitivity to peroxide, as well as to other mediators. In any case, porous glassy carbon can be considered an excellent transducer for amperometric measurements, as is the case of porous carbon, while providing cavities adequate for enzyme immobilization.
By analogy with the control of the density of electron states in semiconductor quantum wells, wires, and dots, it is possible to control the density of photon states by creating a medium with artificially designed regions of varying index of refraction, i.e., photonic crystals (PhCs). Thus, by controlling the patterns of materials on a length scale comparable with the wavelength of light, in one, two, and three dimensions, nanometric structures with designed optical characteristics can be artificially created [
In general terms, photonic crystals are an attractive biosensing platform because they provide strong light confinement. Unlike many sensing platforms that utilize the interaction between the small evanescent tail of the electromagnetic field and the analyte, PhCs can be designed to localize the electric field in the low refractive index region (e.g., air pores), which makes the sensors extremely sensitive to a small refractive index change produced by bio-molecule immobilization on the pore walls. Moreover, by introducing a point defect into a PhC, defect states can be pulled down from the air band or up from the substrate band. The corresponding optical spectra show narrow transmission peaks inside the bandgap, whose precise position is determined by the refractive index of the pores. Thus, the presence of molecules inside the pores can be detected by monitoring a small spectral shift, especially if high-Q microcavities, which have been reported both theoretically and experimentally. However, protein recognition depends on the surface chemistry, thus, instead of filling up the pores and changing the ambient refractive index, the molecules coat the pore walls.
A two-dimensional photonic crystal microcavity biosensor has been demonstrated [
The use of quantum dots in the field of biosensing has been discussed in this review, including issues such as materials, bioconjugation of quantum dots and cell internalization. Additionally, the use of novel materials with nanometric pore size in the field of biosensing has been presented. Also, it was shown that photonic crystals are an attractive biosensing platform because they provide strong light confinement.
The current status of quantum dot technology is expected to evolve in a rapid fashion on many different aspects. The use of novel systems could lead to improved properties including narrower fluorescence emission and longer lifetimes, as well as suppression of blinking and quantum yield enhancement. Also, sensitivity to electric or magnetic fields may play an important role in future biosensor systems.
(A) Emission maxima and sizes of quantum dots of different composition. Quantum dots can be synthesized from various types of semiconductor materials (II–VI: CdS, CdSe, CdTe, etc.; III–V: InP, InAs, etc.; IV–VI: PbSe, etc.). The curves represent experimental data from the literature on the dependence of peak emission wavelength on quantum diameter. The range of emission wavelength is 400 to 1350 nm, with size varying from 2 to 9.5 nm. All spectra are typically around 30 to 50 nm (full width at half maximum). Inset: Representative emission spectra for some materials. (B) Absorption (upper curves) and emission (lower curves) spectra of four CdSe/ZnS quantum dot samples. The blue vertical line indicates the 488-nm line of an argon-ion laser, which can be used to efficiently excite all four types of quantum dots simultaneously. Reproduced from [
Comparison of the size of a representative dihydrolipoic acid (DHLA) capped CdSe/ZnS QD (550 nm emission and diameter ∼ 6 nm), to a maltose binding protein (MBP) molecule (mw∼ 44,000), green fluorescent protein (GFP, mw∼30,000) and a cyanine dye (Cy, mw∼700). Reproduced from [
(A) Schematic of a 4-color multiplex assay. The indicated colors of QDs were prepared with antibodies against the 4-indicated toxins and simultaneously incubated in microtiter-well plates containing the 4-toxins immobilized by capture antibodies on the surface. (B) Multi-toxin assay examining mixes of all four indicated toxins at 1000 ng/mL each probed with a mix of QD-detection antibody conjugates. Measured values are shown as circles. Both the composite fit and the fit from each of the four individual QD components are displayed. Reproduced from [
Operation of the aptamer/QD-based dual-analyte biosensor, involving displacement of the tagged proteins by the target analytes. (A) Mixed monolayer of thiolated aptamers on the gold substrate with the bound protein-QD conjugates; (B) sample addition and displacement of the tagged proteins; (C) dissolution of the remaining captured nanocrystals followed by their electrochemical-stripping detection at a coated glassy carbon electrode. Reproduced from [
(a) Schematic of QD modulation by MBP-BIPS. (b) Spectral properties and modulation function of MBP-BIPS and the 555 nm emitting QDs. Reproduced from [
The principles of a QD-based nanosensor for Rev-RRE interaction assay. (a) Secondary structure of biotinylated RRE IIB RNA. Nucleotides identified as important for Rev binding are shown in red. (b) Sequence of Cy5-labeled Rev peptide. (c) Conceptual scheme of the QD-based nanosensor for Rev-RRE interaction assay based on FRET between 605QD and Cy5. The binding of a Cy5-labeled Rev to a biotinylated RRE IIB RNA formed a Rev-RRE complex, which was caught on the surface of a 605QD to form a 605QD/Rev-RRE/Cy5 assembly through specific streptavidin-biotin binding. FRET occurred between the 605QD and Cy5 upon illumination of the 605QD/Rev-RRE/Cy5 assemblies with an excitation wavelength of 488 nm. Reproduced from [
Schematics of QD nanobarcoded microbead system for high-throughput gene expression analysis. (A) Pseudocolor picture of the microbeads embedded with quantum dots. (B) Example spectra of the beads coded with different mixture of QDs. (C) Construction of the nanobarcoded microbeads. Each bead has a distinctive ratio of four different QDs, allowing identification by a characteristic spectral nanobarcode. The transcript-specific oligonucleotide probes are conjugated to the bead surface. Therefore, each spectral-barcoded bead detects a specific oligomucleotide determined by the probe. (D) Gene expression monitoring and quantification sandwich assay. The nanobarcoded microbead-attached oligo probes capture biotinylated cRNA sample through hybridization, the cRNA is further sandwiched by the 655 nm streptavidin QDs (or 705 nm, 800 nm) to be quantified. Reproduced from [
(Left) Schematic of a PS-based optical interferometric biosensor. The silicon oxide surface of the porous layer can be modified to express various molecular recognition elements (such as oligonucleotides, biotin or antibodies). Reflection of white light at the top and bottom of the PS layer results in an interference pattern (Fabry-Perot fringes). Interactions of the molecular species with their recognition partners immobilized on the surface induce a change in the refractive index of the nanocrystalline semiconductor, giving rise to wavelength shifts in the fringe pattern that can be easily detected (charge-coupled device (CCD) camera) and quantified. (Right) Interferometric reflectance spectra of DNA-modified PS layers. Experiments were performed for two DNA sequences and the corresponding complementary strands. For clarity, only one set of data is shown in each case. (A) The Fabry-Perot fringes from a PS surface derivatized with DNA-A ("before hybridization," red trace) shift to shorter wavelength upon exposure to a 2 × 10−12 M solution of DNA-A' (the cDNA sequence to DNA-A) in 1 M NaCl(aq) ("after hybridization," blue trace). The net change in effective optical thickness (from 7,986 to 7,925 nm) upon DNA-A' recognition is represented by the difference between the two interference spectra ("difference," green trace). (B) The control experiment, showing the Fabry-Perot fringes of a DNA-A–derivatized PSi surface before and after exposure to a 2 × 10−12 M solution of DNA-B (non-cDNA sequence) in 1 M NaCl(aq). No wavelength shift was observed up to the measured concentration of 10−9 M DNA-B. Reproduced from [
Recognition and binding of bacteriophage lambda DNA to a 30-nucleotide complementary DNA sequence immobilized on a silicon chip is detected by a 12 nm red-shift in photoluminescence. The DNA concentration of the sensing bacteriophage lambda is 194.2 fM. Reproduced from [
(Left) Scanning electron microscopy photograph of a typical photonic crystal and schematic of the experimental setup. A tunable laser (1,440 nm to 1,590 nm) is used as the source. Light is coupled in and out of the PhC using tapered ridge waveguides. A polarization controller is used to maximize the TE mode signal, and an InGaAs detector is used to measure the transmission signal. (Right) Normalized transmission spectra of the PhC microcavity. Curve (a) indicates the initial spectrum resonance after oxidation and silanization, curve (b) is measured after glutaraldehyde attaches to the pore walls, and curve (c) is obtained after infiltration of BSA molecules. Reproduced from [
Comparison of organic/protein fluorophore and quantum dot properties. Adapted from [
|Absorption spectra
|Variable/narrow generally a mirror of the emission spectra
|Broad spectra, steadily increases towards the UV from the first absorption band edge
|Molar extinction coefficients
|Variable, generally < 200,000 M−1 cm−1
|High, 10–100 times that of fluorophores
|Emission spectra
|Broad, asymmetric red-tailed emission
|Narrow FWHM, typically 25 to 40 nm for CdSe core materials
|Maturation time
|Needed for fluorescent proteins
|Effective Stokes shifts
|Generally < 100 nm
|> 200 nm possible
|Tunable emission
|Unique to QDs / can be size-tuned from the UV to IR
|Quantum yield
|Variable, low to high
|Generally high, 0.2 to 0.7 in buffer depending upon surface coating
|Fluorescent lifetime
|Short < 5 ns
|Long ∼ 10–20 ns or greater
|Spectral range
|Necessitates a different dye every 40–60 nm
|UV-IR depending upon binary/ternary material
|Variable to poor
|Excellent, strong resistance to photobleaching several orders of magnitude that of dyes
|Multiphoton cross section
|Variable to poor
|Excellent > 2–3 orders of magnitude that of dyes
|Single-molecule capabilities
|FRET capabilities
|Variable, mostly single donor- single acceptor configurations
|Excellent donors, size tune emission to improve the overlap with an acceptor dye, single donor-multiple acceptor configurations possible
|Multiplexing capabilities
|Excellent, largely unexplored
|Intermittency (blinking)
|Maybe problematic in isolated circumstances (single molecule tracking)
|Chemical resistance
|Multiple reactivities commercially available
|Limited conjugation chemistries available
|Mono-valent attachment
|Multi-valent attachment
|Rare – mostly
|Good possibilities, can attach several molecules to QDs depending upon size
|Physical size
|< 0.5 nm
|4 – 7 nm diameter for CdSe core material
|Largely untapped
|Cost effectiveness
|Very good / multiple suppliers
|Poor / few commercial suppliers

subset: commoncrawl


text:
Tuesday, Aug. 23, 2011 | 2 a.m.
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There are several reasons why Las Vegas oddsmakers set UNLV's win total at 2.5 heading into the 2011 season.
They were 2-11 a year ago. Their schedule isn't as tough as it was in 2010, but it's still pretty difficult. They're young. They have plenty of question marks.
After watching the first two weeks of the Rebels' second fall camp under coach Bobby Hauck, I'm of this opinion: UNLV will be more competitive and exciting to watch in 2011 than it was in 2010, but is still about a year away from being a program that regularly contends for postseason berths.
But the program is headed in the right direction, there's no question about that. Signs of it abound when you compare last year's fall camp to the current one. Here are five of them:
Photo by Steve Marcus
1) They're just in better shape
It's not that guys were heaving left and right in camp last season, but the fruits of strength and conditioning coach Mike Gerber's program are visible.
Guys who needed to add weight did so effectively, while others just look more trim and fit. There's a noticeable lack of fat on the bodies of both UNLV's offensive and defensive linemen.
"Absolutely it's better," Hauck said. "(Gerber) always feels like 18 months into a strength program, you start seeing the dividends.
"I think we're certainly in better shape this year than we were in last year. We're able to go longer and harder (in practice), guys are learning to play harder, fewer guys are banged up, all of those things."
Photo by Sam Morris/Las Vegas Sun
2) More productive practices
On top of being able to push guys who are in better shape to practice longer, Hauck can also conserve some energy of his own on the practice field.
"We are spending more time coaching technique and scheme rather than just attitude and effort," Hauck said. "A lot of that is more of a mainstay now, day in and day out, which is good."
Hauck didn't have players from the Mike Sanford era necessarily rebelling against him openly last fall, but he certainly had some hurdles to clear in terms of getting guys to fall in line with his approach. His style at Montana was hard-nosed and disciplined with a strong hint of old-school philosophy.
It's beginning to show within the current personnel. There's still a ways to go in that department, but not as much as there was a year ago.
Photo by Sam Morris
3) Efforts on the recruiting trail are showing
It was going to be hard for Hauck and his staff to come into the UNLV program last year and immediately target top-flight prep recruits and reel them in.
Instead, they followed a more challenging pattern: Comb the junior college ranks for guys who can play right away, then take high school prospects who need some work — especially those in their backyard — but could develop into strong college players.
Among linebacker Princeton Jackson, cornerback Ken Spigner and safety Dre Crawford, Hauck hauled in three juco prospects this year who will likely see serious playing time right from go in 2011 and help shore up a defense that was thin in numbers last season. Several more could see the field, too.Meanwhile, some of the under the radar high school seniors the staff saw potential in a year ago are now looking like key contributors.
A good example is sophomore running back Tim Cornett, who led the Rebels in rushing last year with 546 yards. Now, he's transformed himself into more than just a speedster who can rip off big runs. He bulked up, learned how to run between the tackles more effectively and has the look of a complete back.
The next to emerge could be redshirt freshman left tackle Cameron Jefferson. He was raw coming into UNLV a year ago. He only played two seasons of football at Desert Oasis High, tipped the scales at just over 260 pounds and had rough technique. Now, he's up to 290 pounds while maintaining his above-average athleticism and looks much more comfortable at the position. He'll learn on the fly this season and likely will endure some visible growing pains, but expect him to anchor UNLV's offensive line for several years.
There will likely be several stories similar to Jefferson's as the staff continues to look for potential rather than polish on the recruiting trail. And landing some of those guys in both the 2012 and 2013 classes now becomes a bit easier that there's stability at the top of the program, with Hauck having agreed last week to an extension through the 2014 season.
Photo by Sam Morris
4) Stability at the quarterback position
In a move that was surprising to very few, Caleb Herring was officially named UNLV's starting quarterback on Sunday.
Yes, he earned the job so far this fall camp by showing the best knowledge of the Rebels' offense and by being the most consistent performer of those competing for the gig.
But, really, he also earned the job last year. Aside from gaining valuable experience by appearing in eight of UNLV's 13 games, he successfully made the difficult transition from being a quarterback in the shotgun spread offense to a guy who can operate from either the shotgun or under center.
He was successfully reprogrammed, and now has both the sound and the look of a guy who is more confident in what he's doing. Add in the fact that he's only a sophomore, and, barring injury, UNLV could have some stability at football's most important position for the next three seasons. As many who follow UNLV football know, that hasn't been the program's strong suit in the past decade or so.
Photo by Sam Morris/Las Vegas Sun
5) Then there's scheduling
OK, so this one is not related to training camp. But the proof should show up on paper in the future.
Last season's schedule was originally set up by former athletic director Mike Hamrick with the idea that the program would be peaking under Mike Sanford and be ready to step up to bigger challenges (and paydays). Instead, in a transition year under a new head coach, the nonconference slate included Wisconsin, West Virginia, Nevada-Reno, Hawaii and Idaho. Mix in BYU, TCU, Air Force, Utah and San Diego State in the conference portion of the slate, and, well, Hauck was in a tough spot.
So expect the philosophy to change some in the coming years. The hope for UNLV is that while the quality of the personnel increases, the level of competition from outside of the Mountain West Conference will even out some.
For example, next season, UNLV will host Minnesota and Washington State — two lower-ranking teams from BCS conferences — while traveling to face Utah State and Louisiana Tech. All four could be looked at as games the Rebels will have a chance to win.
The idea of playing the likes of Wisconsin and West Virginia might seem more sensible if the upward trend continues.
But not right now.
"(In 2010) we played a Top-15, Top-20 schedule in the country, were the only non-BCS school to play a schedule like that, and the reality of it is if we want to turn this around, we have no business playing the schedule we played last year," he said. "It makes zero sense. It just doesn't."


text:
Home Plan Detail
By: Jon Rentfrow
Rentfrow Residential Design
Square FootageTotal living area: 2788' Basement: 4118' Main Level:2124' Upper Level:664' Basement:1330' Footprint: 51' 4" W x 60' 0" D
RoomsBedrooms: 3 Bathrooms: 2.5 Master suite location: Main Floor
AttributesLevels: 2 Parking: Garage Number of car stalls: 2 Foundation:
- Wraparound Porch
- Vaulted Ceiling
- Fireplace
- Optional Basement
- Skylight
After entering the home off of the covered front porch, you find yourself in a foyer space with a vaulted ceiling that reaches over 15' in height. To the left there is an arched opening to a formal dining room, which has two pairs of French doors that open onto the front porch. As you walk down the foyer hallway, you pass a stairwell and a vestibule. The vestibule has a nook with a counter, a prep sink, and an under counter refrigerator conveniently located across from the dining room, and a powder room. After continuing down the foyer hall, an arched opening straight ahead looks into an open family room with a 14' high ceiling. A fireplace is against one wall, along with built–ins for media and books. Two pairs of glass doors with picture windows above them look out to the backyard patio, and bring in abundant natural light. The room is open to a sunny breakfast nook, which has an eating counter that separates it from the kitchen. The kitchen has a center island with a stove, a vaulted ceiling, and space for built–ins, such as a desk and window seats, along the window wall. The kitchen also has a corner pantry, and a small vestibule with coat hooks and a door that opens into the two–car garage. On the other side of the family room, a hall leads to a laundry room and the master suite. The master bedroom has a vaulted ceiling, and windows along three walls. The master bath has room for both a tub and a shower, and the walk–in closet has a secondary door to the laundry room for easy access.
Upstairs, the second floor landing is oversized and light–filled from a skylight above. There is a bedroom, a full bath, and a den, which may also be used as a third bedroom/guest room if needed. A door from the bedroom closet accesses the attic for additional storage space.
The optional basement plan has an open recreation room with a fireplace, a bedroom, a full bathroom, and plenty of storage.
Floor Plans


text:
Uncyclopedia:Pee Review/Final Destination
From Uncyclopedia, the content-free encyclopedia
edit Final Destination
Mostly total overhaul of the Final Destination article, which started off crappy, then became crappier (namely because of me). I've come to apologise. EpicAwesomeness (talk) 16:36, July 2, 2011 (UTC)
|I like what I'm seeing here, well so far anyway, some minor issues but mostly sone really funny stuff in this article. I'll go through it in depth now:
Introduction - This section is exremely well done, a carefully executed section of humourous sarcasm to introduce your topic as well over exaggerating it slightly, but enough to produce the humour it needs without overdoing it. The quote used could stant for a little improvement (too long, it could be perhaps made more brief whilst still getting the point across). But all in all a well done introduction.
Production - This bit is also well done, combining: Fact, sarcasm, satire and a tiny bit of crudeness towards the producers. I like the way this is structed, and if you came here looking for a way to improve it, sorry to disappoint but I can't it's perfect and any attempts to improve it would probably ruin it.
Popularity - I like this section too, but it could use a minor polished in the sentence structuring, what I mean is merge certain sentences together to avoid unfunnyly repeating yourself, otherwisae its like the rest a good combination of truth and humour and not over done in the slightest.
Fifth film and Themes - These I think were the downfall of this article and stopped the score edging from 8.5 towards 9.5 or a 10. They need more content and pictures to accompany them. Images give not only a visual representation to the reader but also humour through backing up what the reader sees in the text.
Spin-offs - A slight poisoning by being random, but this is largly well done satire. Improving this section is the least of your concerns, improving the above two sections is what you should try and focus on.
Your humour is very good, but I can see that it could potentially be better than it is.
|The concept is reasonbly well developed, but lacks one important point characters. Due to the style of thi acticle, you should have included some information on the characters. Do this and has potential to be really funny. Ive said it before when doing reviews, humour and concept go hand in hand:
Good concept = Funny article
Poor concept = Not so funny article.
Lastly in the sections I was harasher in with the humour, you need to imprrove the concept of it. So get your concept more detailed and the humour within this article will improve up to a full score!
|Prose and formatting:
|I like how you used references through your article, they show good formatting skills and understanding of the subject matter. Just a couple of minor fixups needed:
Needs more links - I think it doesn't have enough, don't over link your article and keep them well distributed, but add a few more here and there.
And a minor issue what the hell is with the inroduction, the last 4 words got cut off, or has that got something to do with the template?
|The images you've picked are really funny and are of a high quality. But as I said the latter two section need expanding and have images to accompany them. So when expanding them try and find appropraite images to use here.
|My overall rating out of 10
|Final Score:
|So yah, largly a well done article. But it needs a slght amount of work on it, I see a potential feature here if you keep working on it!
|~Sir Frosty (Talk to me!) 05:15, July 7, 2011 (UTC)


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SYTYCD Recap: Wraps Up Auditions with Redemption, Anger, Great Dancing
This week's So You Think You Can Dance marked the end of an era. After an all too short month, DANCE fans bid a fond(?) adieu to Justin Bieber and his choreographer Nick DeMoura, who introduced their final Dance Crew Battle. While I'm sure Uncle Nigel will do whatever he can to get The Biebs on the finale to introduced the most-tweeted crew, assuming all of his visa issues are resolved.
Also coming to an end this week was the Season 11 auditions. From the gorgeous Fabulous FOX Theatre in Atlanta (where I have seen many-a-show) Hip-Hop icon LiL Buck and STEP UP star (and MAGIC MIKE's wife) Jenna Dewan Tatum served as guest judges alongside Mary Murphy and the aforementioned Nigel Lythgoe.
We got a little bit of everything this week; redemption stories, angry tirades, the patented sob-stories, the always annoying kid-dancing segment, and, as expected, some phenomenal dancing. So, onto the auditions:
Mariah Reives
Sanford, NC
Style: Jazz
Three-Word Review: Damn, she's good!
Result: Ticket to Callbacks
Erik "Silky" Moore
Orlando, FL
Style: Hip-hop
Three-Word Review: Fantastically Vivid Musicality
Result: Ticket to Callbacks
Kelly MacCoy
Cincinnati, OH
Style: Jazz
Three-Word Review: Fun, "Trick"y Number
Result: Ticket to Callbacks
Chris "Mr. Strange" Griffin, Founding Member of Dragon House
Atlanta, GA
Style: Animation
Three-Word Review: Crazy Cool Angles
Result: Ticket to Callbacks
Conrad Dechabert
Austin, TX
Style: Contemporary
Three-Word Review: Pretty Darn Manly
Result: Ticket to Callbacks
Taveaus "Dynamic" Woods
Las Vegas, NV
Style: Hip-Hop
Three-Word Review: Talented Little Dude
Result: Choreography (Eventual No)
Brandon "Sideshow" Feimster, Dragon House member
Detroit, MI
Style: "True Pop-Locking"
Three-Word Review: Boring, Anger Issues
Result: No
Ricky Ubeda
Miami, FL
Style: Contemporary
Three-Word Review: Little Dabble Do
Result: Ticket to Callbacks


text:
Know Van Halen? Take Rolling Stone's quiz
Fun feature from Rolling Stone magazine, which has posted a quiz to test fans on their Van Halen trivia skills. Check for the link to the quiz farther down below. But just a few quick notes. The hit rockers recently reunited, with David Lee Roth and brothers Eddie and Alex… Read More →


text:
One of Australia's largest real estate networks is facing internal upheaval amid allegations of mismanagement, manipulation of books and possible conflicts of interest including deals with suppliers owned by or linked to its own board members.
In recent months, a reform group of disgruntled First National Real Estate agents has called for a forensic audit of the company's books for the past five years under the company's chief executive, former Melbourne Football Club boss Ray Ellis.
As part of the push for change, a former finance manager has written to the corporate regulator admitting his role in falsifying First National's accounts to improve the company's financial position.
In a confidential 2014 letter to the Australian Securities and Investments Commission obtained by Fairfax Media, former finance manager Darren Moon says that in the years 2010-2011 he was "required" by Mr Ellis to "adjust" financial reports and "overcapitalise the balance sheet by manufacturing figures". During 2012-2013, First National was teetering on insolvency.
Mr Moon's bold admission is one in a long list of allegations of financial irregularities against his former employer. He resigned in 2013.
Mr Ellis and his board have refused to conduct a forensic audit, and have silenced the reform group - believed to be supported by about 40 agents - including through thinly veiled threats of legal action.
Both First National chairman David Nitschke and Mr Ellis refused to be interviewed by or to answer questions from Fairfax Media.
In the minutes from the company's 2014 annual general meeting, Mr Ellis acknowledges manipulation of accounts, while denying fraud, misappropriation of funds or "wrongdoing of any nature".
Instead he blames Mr Moon, claiming the former finance manager acted "independently" in choosing to "overstate the value of assets in the balance sheet".
Curiously, given the seriousness of such malpractice, First National appears to have taken no action against Mr Moon.
First National is a member organisation formed in 1981 which has hundreds of branches across Australia and New Zealand. It spruiks itself as Australia's "most advanced real estate network" .
At the time of Mr Ellis' appointment in 2004 the business claimed 580 member offices.
In 2012-2013 the First National was in financial trouble, with its survival apparently dependent on an inflated balance sheet, an outdated valuation of a major investment, a Westpac overdraft, and large personal loans and lines of credit from Mr Ellis.
The First National website now claims 400 members. Critics inside the network insist real membership is much lower.
A major question hangs over First National's decision in 2011 to buy a quarter stake - valued at the time at almost $763,000 - in software company Multi-Array Pty Ltd.
While the company has repeatedly valued the Multi-Array shares at cost, $762,750, First National agents and former senior employees have told Fairfax Media they fear Multi-Array is now worth a fraction of its original value.
As a result, for three consecutive years, 2012-2014, auditors Crowe Horwath handed down qualified audits, noting the Multi-Array shares should have been valued at market, not cost, value.
The former finance manager and the reform group raised concerns about a string of deals between First National and suppliers with clear links to present or past board members.
They include more than $260,000 paid to training company Australian Property Compliance, owned by Frank Tierney, a Mildura agent who was First National chairman when Mr Ellis was appointed chief executive in 2004.
Company insiders say that payments to Australian Property Compliance were never budgeted for, and they have questioned the extent of training provided. Fairfax Media understands that one director resigned from the First National board in 2013 in protest over the company's failure to justify the payments to Australian Property Compliance.
Other deals of concern include payments to software supplier Sellercom Pty Ltd, whose directors include First National directors David Hamilton and Michael Gray, and to a related company, Bluerock Software.
A senior First National source assured Fairfax Media that all appropriate declarations had been made by board members and recorded in board minutes. The minutes are not publicly available and First National refused to release them to Fairfax Media.
New auditors Grant Thornton are finalising the 2014/2015 financial report, and have been made aware of the members' concerns and allegations.
In early 2004, Mr Ellis was asked to resign as Melbourne Football Club chief executive after just one year in the job, and a projected profit in 2003 turned into a $2.2 million loss.
On Tuesday morning, First National chairman Mr Nitschke issued a general statement in which he notes the tendency for disagreement within member organisations such as First National. "The board acknowledges the intentions of some members to advocate for change and we are pleased that those members are engaging with the board and the greater membership for the benefit of all our many stakeholders," it read.


text:
The best news here at the Briar Patch Inn is always related to our lovely guests. They are the reason we exist and why our job is so enjoyable. Each person that chooses to stay in this secluded place (without phones or televisions in the cottages and barely any cell phone reception—true amenities!), is usually with us to revel in the spirit of connection to nature and, most importantly, to connect with their own inner beauty. Here, one can truly feel as if they have stepped into a sanctuary where their soul is able to take a deep, contented breath.
With that said, we felt that no one could inform others who are considering a stay with us more eloquently than those who have experienced this lush and enchanting wonderland firsthand. We have a guest book in which people can share their feelings about their time at the Briar Patch Inn. So we decided to devote this newsletter to what they have to say…
"A wonderful break from our normal, fast-paced lives."
--Brandon and Celeste
"We love and so enjoy your peaceful ambiance and spiritual serenity."
"Falling in love again."
--Kris and Patrick
"Got engaged!!! Most magical place on earth. Thank you!"
--Dan and Constanza
"First time, will be repeat offenders."
--Tom and Lisa
"We love this place so very much. It's our special Thanksgiving tradition.
Thank you for creating such a beautiful and enchanted spot for us. We love you."
--Mashburn Family
"Do we HAVE to go home?"
--Kari and Keilen
"I came to find answers and found the Divine."
"She said YES!!"
"The Deckhouse was so perfect!
And the courtesy, help and attention offered by staff was beyond wonderful!
Finally, the breakfasts and treats—outstanding."
--Shonnie and Barbara
"Nice to know places like Briar Patch still exist . . . so peaceful."
--Dave and Kathy
"Words cannot describe how happy we were to arrive at this blessed place of healing."
--Anderson family
(Would like to mention that this family had been hit by a light rail train on their way to us.
Their healing time here was much needed. Beautiful family!)
"Perfect, only better."
Thank you all for making Briar Patch a special place!
See you soon . . .
The Briar Patch Family


text:
Class B Training
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|Comments (1)
Always #2 Never #1 in Alliance, Ohio
55 months ago
Anybody know any companies in The Northeast Ohio Area, Preferably Stark, Summit, Tuscarwaras and surrounding Counties that pay for your class B cdl to work for them. that would be no experience, just apply as a normal job? I.E. trash companies and other places
» Sign in or create an account to comment on this topic.


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Once visitors end up on your landing page, you're no longer competing for their attention with other Web sites, so change the focus to the task they're trying to accomplish. You're missing an enormous opportunity by not creating a hype-free zone on your landing page.
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When a small business fails at search marketing, more often than not the problem boils down to a poorly thought-out business plan. Monetizing a Web site is so much more than slapping some ads on a page and waiting for the checks to roll in. You need to create a business plan, and test viability of that plan before you spend any money on marketing and development.
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Posted by Nathania Johnson Jan 22, 2009
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Posted by Nathania Johnson Jan 22, 2009
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| Delve Networks Releases Impressive Obama Inauguration Speech Video Search
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Delve Networks has released an awesome tool that lets you search Obama's inauguration speech video. When you type in a keyword, the parts of the timeline where the keyword was mentioned are highlighted. Here's a visual: Next, when you put your mouse over one of the highlighted areas, a bubble appears …
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text:
The Art of Cinema #427
The Red Badge of Courage
(John Huston; 1951)
|your thoughts:
6 comments:
- Christopher said...
a one of a kind powerhouse of a movie,even in all its troubled shortened form..
- December 28, 2009 12:30 AM
- 42nd Street Memories said...
I agree. Underrated film. Also proved that Audie Murphy could act when guided by a top notch filmmaker.
- December 28, 2009 7:44 AM
- Tod Brody said...
Is that the cartoonist Bill Mauldin? Didn't know he was an actor.
- December 28, 2009 12:48 PM
- Christopher said...
yep its the same Bill Mauldin
- December 29, 2009 12:46 AM
- farouchegrande said...
if you manage to find the book "Picture" by Lillian Ross (republished by Da Capo Press a few years ago), don't miss it.
upon Huston's request, Ross followed the whole creative process behind this movie from the script to the premiere, and there's a lot more in it than you see on screen. one wonders, if the movie is what it is after all the changes MGM forced upon it, what a masterpiece would it be if they let it be as JH conceived it...
- December 31, 2009 9:33 PM
- Gerard Saylor said...
Mauldin was not an actor. I read a recent Mauldin biography that said Murphy disliked Mauldin because Mauldin was an artist.
I think Max Allan Collins mentioned before - it may have been here - that he partly modeled a character after Murphy.
- January 03, 2010 8:18 PM


text:
CAREER Model for Career Development Success
by Career Coach Randall Hansen Ph.D.
Can anyone guarantee career or job-search success? Of course not. Searching for a career or job is a journey and a journey that often takes many unexpected twists and turns. Sometimes success is right around the next bend while other times it is quite a way down the path.
What can help job-seekers with this career and job-search journey? Job-seekers with a plan almost always fare better than those job-seekers who muddle through the process. With this concept in mind the CAREER Model for Career Development Success was developed by the team at Quintessential Careers.
CAREER Model for Career Development Success
|Contemplate and understand who you are and what you want from life. This first step is most critical and can sometimes take the most amount of time.
|Assess your current situation and what you want to become. This step involves self assessment in a variety of ways as you begin to develop a vision of your next career move.
|Research and set goals to achieve the vision of yourself. Research is critical for all phases of your job-search and in this step you need to fully research the requirements of your next career move.
|Explore and develop short and long term plans to achieve your goals. This step builds on the assessment and research from previous steps and has you mapping out a plan to achieve your career goal.
|Execute and implement your plans making changes where necessary. Once your plans have been formulated the next step is actually putting the plans into action making necessary changes as you progress toward your career goal.
|Reflect on success; plan for future. With your career goal in sight this step is about rewarding yourself for achieving your career goals while also looking ahead and continuing the process to achieve your longer-term goals and aspirations.
If you are ready to implement the CAREER Model in your life your next step should be to take advantage of these free career resources.
Are you part of an organization or group? Invite Dr. Hansen to present one or more Empowering Workshops and Presentations.
Go back to Why Use a Career Coach?
Read about the Benefits of Coaching.
Read more About Career Doctor Randall Hansen.
Dr. Randall Hansen is THE Career Doctor. He is also is founder of Quintessential Careers one of the oldest and most comprehensive career development sites on the Web as well CEO of EmpoweringSites.com. He is also founder of MyCollegeSuccessStory.com and EnhanceMyVocabulary.com. He is publisher of Quintessential Careers Press including the Quintessential Careers electronic newsletter QuintZine. Dr. Hansen is also a published author with several books chapters in books and hundreds of articles. He's often quoted in the media and conducts empowering workshops around the country. Finally Dr. Hansen is also an educator having taught at the college level for more than 15 years. Learn more.
The Career Doctor is part of the Quintessential Careers Network of Career Sites. Quintessential Careers is one of the most comprehensive and award-winning career development sites on the Web empowering job-seekers since 1996.


text:
KINGSWOOD'S eight game unbeaten run was brought to an end by Kings Stanley.
The home side were the better team throughout and should have taken the lead early on when a one-on-one situation involving Liam Gale and goalkeeper Chris Fox ended with Fox making a really good save with his legs.
Stanley did take the lead in the 20th minute when Steve Robertson's shot was blocked, but rebounded for Danny Stephens to fire home.
In the second half Fox was in action again as he pulled off another good save from an Andy Maryon header and then minutes later denied Robertson.
A second goal late on by Stephens settled the result in favour of Stanley.


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Frolic Fabric Collection Tour
June 1, 2017
It's officially summer break for me and the kids and I'm looking forward to two months of fun, relaxation, and hopefully some qu...8 comments
It's officially summer break for me and the kids and I'm looking forward to two months of fun, relaxation, and hopefully some qu...8 comments
I'm taking a quick break from my blogging sabbatical to share this Polina Dress I made for the Coffee + Thread Pattern tour! My fr...14 comments
Hello there! Today I'm sharing this duffle bag I made using a pattern by Virginia of Gingercake Patterns . I've known Virginia ...13 comments
It's that time of year again! As a thank you to all of you, I'm having my last big sale of the year just in time for those of yo...1 comment
*post contains affiliate links Michelle Patterns were some of the first pdf sewing patterns I used when I discovered the onlin...No comments
I made this London Backpack and these zipper pouches earlier this year as quilt market samples and am sharing them today as part of t...1 comment
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text:
The Shakespeare Conference: SHK 12.0346 Tuesday, 13 February 2001
Date: Monday, 12 Feb 2001 13:20:12 -0500 (EST)
Subject: Hamlet and Oedipus?!?
Clifford Stetner refers to "unresolved Oedipal" issues Hamlet may have
with Gertrude. . . frankly, I wonder that we still haven't gone beyond
Dr. Ernest Jones' type of critique, which I find so willfully
superficial and unscientific that it should have been ditched long ago.
Is Stetner saying that there can be no other reasons, dramatic or
otherwise, for Hamlet's intemperate response to his mother's
re-marriage? Is he implying that the _only_ reason Hamlet lingers on
the time it took for remarriage was because he lusts for his mom? I
should certainly hope not.
If we are going to apply a scientific model, and we need to consider
Freud as a scientist (I know, that's a big if, but what can we do), then
the only way to prove the Oedipal theory is to take into account the
facts of the case, and come up with compelling reasons why other, more
down-to-earth theories don't apply. Here's my theory:
It doesn't take Freud or Jones to realize that step-parents are rarely
welcome. And if the new dad happens to be a drunken bastard of an
uncle, making the marriage not only ill-advised but _incestuous_ in the
eyes of any Church in Hamlet's day, I fail to see any reason for Oedipus
to intrude on the discussion. Hamlet's humiliation at his mother's
conduct can be easily understood by anyone who looks the situation
squarely in the eye. The original legend makes clear that Hamlet is
disgusted with the Queen's quick remarriage -- Shakespeare adds that
touch of pathos that he is also embarrassed for her, and the
embarrassment comes precisely because he remembers what a great
wife/queen/mother she used to be.
How simple humiliation and disgust can be twisted into carnal lust is
beyond me, and has yet to be proven. No matter how fine a stylist Jones
was, he was working from a deliberate misunderstanding of the play. He
doesn't even understand that, for Hamlet, revenge can never be just
killing off Claudius. (Do we need to go over the concept of
damnation-as-revenge again?) The hesitation in the chapel, again, is
perfectly understandable given the values of Shakespeare's time (and, if
you're a Baptist, even our own).
Sorry, but this one puts a bee in my hat, and I had to respond.
Andrew White


text:
Doctor insights on:
My Upper Abdomen Itchy Skin
Yellow bruising mostly on arms. Yellow watery diareha. Seeing the color yellow. Some abdomen discomfort.Itchy skin?
Yellow: The cause can be something as simple as eating a lot of foods high in vitamin a (carrots, yellow peppers) or as serious as hepatitis or liver cancer. See your doctor for an evaluation of your liver. ...Read more
Upper Abdomen (Definition)
Draw a transverse line through the umbilicus and the upper abdomen includes every part of the belly above this line and below the ...Read more
Can mono cause high liver enzymes, itchy skin (no rash), gallbaldder thickening, pain in lower abdomen and upper lower back and anxiety?
This sysptoms started 2 months postpartum.
Partly: See your doctor for work up to get the exact answer. ...Read more
Have dull upper right abdominal pain which i feel every half an hour or so. and Itchy skin. Thanks so much?
Gallbladder: Sounds like the possibility of a gallbladder issue, stones or inflammation. Definitely important to have an examination and likely some blood tests and an ultrasound. ...Read more
I had abdominal pain, slight feeling that my whole body is trembling and frequent itchy skin immediately after eating what should I do???
Allergy?: If your symptoms of abdominal pain, itchy skin and shaking is related to a specific type of food ingestion, this could be an allergy to that food. It would be unusual for these symptoms to occur after eating in general. ...Read more
My stool has been orange about 4 weeks. I don't have abdominal pain. no fatigue, just itchy skin sometimes. and I don't have jaundice, what is wrong?
Acholic stools: are frequently associated with gall bladder disease? your young, & don't seem to have any other symptoms listed- Do you eat a lot of carrots?- yours are not clay colored?? ...Read more
If I have upper right abdominal pain and itchy skin, and my doctor ordered a liver fiction test panel as well as an ultrasound; will these show an autoimmune hepatitis?
They will: provide evidence for or against the diagnosis, either helping to confirm it or ruling it out. ...Read more
Is there any STD that cause joint pain, loose stool, abdominal pain, itchy skin?
Some may: Hiv can, sometimes gonorrhea can cause some of those symptoms. ...Read more
I have dull upper right abdominal pain. Itchy skin fatigue and diarrhea. All my blood test, ct, ultrasound comes back negative. What should I do?
RUQ anatomy: Anatomically, the right upper quadrant (RUQ) of the abdomen includes: liver, gallbladder, pancreatic head, bile ducts and ampulla, c-loop of the duodenum, stomach antrum and pylorus, the hepatic flexure of the colon. Don't forget the diaphragm and lower chest are pretty close by. The abdominal musculature, ribs, and skin can all contribute as well to localizing RUQ pain. Re-exam is appropriate. ...Read moreSee 1 more doctor answer
Is yellow diarrhea, constipation, and mild abdominal pain common a week before menstrual cycle? It started happening after I changed to a healthy diet and more exercise. Are itchy skin spots linked to periods as well? Thanks!
Probably not: Most likely cause would be your dietary change; even potential possibility of a food allergy. It may normalize, but don't hesitate to be seen and thoroughly evaluated. ...Read moreSee 1 more doctor answer
Have a red itchy skin rash on belly and arms?
Rash: Rashes are difficult to diagnose without being seen. Is it flat, pebbly, blistery? How long present? How large an area involved? Any contact with poison ivy personally or a pet? Any exposure to ticks? Any exposure to measles/chickenpox/etc? See pcp for evaluation & treatment of rash. ...Read moreSee 1 more doctor answer
Itchy Skin (Definition)
Often pertains to skin that is dry and ...Read more
- Talk to a doctor live online for free
- Center upper abdomen itchy skin
- Upper left abdomen itchy skin symptoms
- Itchy swollen my upper abdomen symptoms
- Ask a doctor a question free online
- I have itchy skin on my upper back
- Itchy right upper abdomen
- Itchy spots on left upper abdomen
- Itchy white center upper abdomen
- Talk to a dermatologist online


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text:
Barack Obama is confident that the two 'American Idol' female judges, who are big supporters of Democrat, will come to an understanding and move forward instead of backward.
President Barack Obama has weighed in on Mariah Carey and Nicki Minaj feud on "American Idol". In a phone interview with radio host Michael Yo on "The Yo Show", the commander-in-chief is positive the two female judges will be able to solve whatever problem that comes between them.
"Can you repair the relationship with Mariah Carey and Nicki Minaj on 'American Idol'? That's what people want to know," Michael asks Mr President about the "We Belong Together" singer and the "Starships" femcee who are both self-proclaimed supporters of Democrat.
"I think that they are going to be able to sort it out, I am confident," Obama replies. "I'm all about bringing people together, working for the same cause. I think both outstanding artists are going to be able to make sure that they're moving forward and not going backwards."
Although Mr. President gives a very diplomatic answer, he does have a preference of the two artists. "Mariah, she's actually done some events for us. I've gotten to know her and Nick (Cannon), she's a wonderful lady," he says. "Nicki I don't know, but I've got her on my iPod."
Obama also talks about his lackluster performance at the first debate against GOP presidential candidate Mitt Romney. "There's no doubt that I had an off-night," he admits before adding, "He had a sharp presentation but his ideas are still ones that aren't going to work for America and aren't going to work for your listeners. And so we're just going to keep on going."


text:
Former boxer Fred Radmer keeps on fighting
Win or lose – and of his more than 40 amateur fights, he lost only a handful – Fred Radmer was well known and popular for the all-out effort that was his trademark in the boxing ring.
Forty-six years ago, Milwaukee Sentinel sportswriter Ray Grody even anointed the baby-faced 112-pounder as "The People's Choice" when Radmer, then 16, fought his way to the finals of the state Golden Gloves tournament. He lost a close decision in the championship bout at the Milwaukee Auditorium that year, but took home the trophy designating him as the "Outstanding Fighter" of the whole tournament in the novice division.
Later, Radmer won two Golden Gloves state titles, and in 1969 he became a member of the Milwaukee Bombers, the team of elite boxers that represented Brew City in the short-lived International Boxing League and was – in those days before the Brewers, the Wave and the Admirals joined the list – one of only two big league sports franchises in town, the other being the Milwaukee Bucks.
Now 61 and living in West Allis, Radmer is still an indomitable fighter, only since he hung up his boxing gloves more than three decades ago the competition has been even tougher – racism, ignorance, political charlatanry, alcoholism, illness.
He grew up on North 38th and West Brown Streets, where as a boy he and his friend Jerry LaMoore (also later a Golden Gloves champion) sparred in Radmer's backyard. Fred's dad, Ken, had boxed in the U.S. Coast Guard during World War II, and also fought in the Golden Gloves in the early 1940s.
"I always liked boxing," says Radmer. "I learned self-reliance and how to take care of myself."
He liked it so much that in 1965, when he was 15, he said he was 16 – the minimum age – to enter the Golden Gloves tournament then held in Kenosha. After stopping his first opponent Radmer lost in the regional finals, but Evans Kirkby of The Milwaukee Journal wrote, "one of the best fights of the night came ... when Duane Priske of Kenosha outpointed Fred Radmer of Milwaukee in a battle of handsome little 112 pounders."
When the Gloves moved to Milwaukee the next year his legend achieved full liftoff. By '69, when Radmer became a charter member of the Milwaukee Bombers, he had grown into a handsome little featherweight (126 pounds).
The International Boxing League was the brainchild of broadcaster Jack Drees, whose original idea was to establish teams of professional boxers in select major cities around the country that would fight each other in a round-robin of meets. Wes Pavalon, who'd brought the basketball Bucks to town in '68, headed the Milwaukee IBL franchise, but when it was decided to start the league off with amateur rather than professional boxers, Pavalon bailed and the Bombers were taken over by Racine businessman Allen Buhler.
The other IBL franchises were in Chicago, Denver, Detroit, Louisville, Miami, New York City and St. Louis. When the Bombers debuted at Louisville on Nov. 10, 1969, Radmer won the team's first victory in the ring.
The concept of big league boxing wasn't a winner here. Home meets at the Arena and Auditorium drew sparse crowds, and after finishing with a record of 4-5-1 in their first season the Bombers folded. The IBL itself went down for the count a year later.
The padded headgear amateur boxers are required to wear today wasn't an option when Radmer was fighting, but thanks to his hit-and-not-be-hit strategy he was never hurt in the ring. By the time he quit boxing in 1977, though, Radmer was into a more dangerous kind of belting.
"My drinking was getting out of hand," he says. Fred has been sober for the last 15 years, for which he credits the person he proudly calls "the moderating force of my life." Kathy Radmer assumed that role the first time Fred came to see her at her West Allis home, and stole a car to do it.
"I did a lot of dumb things when I was younger," he says.
"He didn't steal any more cars after he met me," says Kathy. Page 1 of 2 (view all on one page)
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Introduction Edit
JapanSoc is a social bookmarking website for Japan-related news, stories and information. It was started by Nick Ramsay in November 2007, and by February 2008, had over 50 registered users. JapanSoc was created as a community building tool, bringing people with an interest in Japan together, and giving them a forum to introduce, vote on and discuss articles with a Japan theme.
JapanSoc was officially launched on November 11th 2007, with the article, JapanSoc Brings Social Bookmarking to Japan, introducing the site and explaining how its development was inspired by other social bookmarking websites and the Pligg content management system.
Benefiting Readers Edit
Social bookmarking is a system whereby the users vote for articles they deem useful, interesting, or simply worth sharing. This method of ranking and filtering submissions usually means that the most popular articles are worth reading, saving time and bringing quality content to the attention of the reader. Subscribing to the JapanSoc RSS Feed is a great way to keep abreast of what's happening in the community.
Helping Bloggers Edit
A successful social bookmarking website with hundreds or thousands of users is a powerful tool for bloggers. Submitted articles that interest JapanSoc users can send waves of traffic to each blog, increasing the readership of each individual blog. Even in its early stages, JapanSoc can Boost Blog Traffic by giving alternative keywords to search engine such as Google. Bloggers are encouraged to register and submit their Japan-related articles to benefit both themselves and the community as a whole by providing useful or interesting reading material.
Promotion Edit
Japan bloggers have been working hard to give JapanSoc the exposure it needs to be a success. Tools such as the JapanSoc Feedburner FeedFlare , a Voting Button, the Nipponster Toolbar, an OS X Widget and Japanopedia's Checklist of 140+ Japan-blogs for JapanSoc are examples of how the community is rallying around JapanSoc. There is a JapanSoc Tools page containing links to all the promotional tools available, and banners and graphics that bloggers can use to show their support can be found on the JapanSoc Banners and Buttons page.
More Information Edit
Learn more about JapanSoc here.


text: Kohl, John - On 29 May 2008, NASA announced its selections for Small Explorer Missions. John is the Lead Investigator for the Smithsonian Astrophysical Observatory (SAO) portion of the CPEX mission, which is a collaborative project of the Naval Research Laboratory and SAO. The six missions selected for Phase A studies will compete for the two mission slots that will be down selected for development.


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Jubilarian – 2010
Helen Hendricks, SSND
Professed in 1960
Sister Helen Hendricks celebrates 50 years as a School Sister of Notre Dame. She became acquainted with the SSNDs in grade school and went on to become archdiocesan teacher of the year.
A meaningful time in my ministry…
In 1985, the members of my class were given a trip to Rome and Germany in celebration of our silver jubilee. This was a very memorable time for me. Mother Theresa of Jesus Gerhardinger was beatified that year, and I especially treasured standing on the gravesite of our community's foundress.
Another meaningful time for me was in the 1970s, when I was named teacher of the year in the Archdiocese of Milwaukee. I was proud of this achievement and happy that my mother came down to Milwaukee by bus to see me accept the award.
What drew me to the SSND Community…
I had two aunts and cousins who were SSNDs. I also had SSNDs as teachers throughout grade school. The Sisters were (and are) very kind and generous people.
One thing you might like to know about the School Sisters of Notre Dame…
You can always recognize an SSND because of her love for community.
In my current ministry…
My current ministry is in Community Service at Our Lady of Mount Carmel Retirement Community, in Mount Calvary, Wis. I do many things, but mainly handle the switchboard and relate e-mail communications to the Sisters. Recently, I also became part-time secretary for the SUNSEED Eco-Education Center, an exciting ministry of the SSNDs.
Looking back and looking ahead…
I am thrilled to be a jubilarian. Who would have thought I'd see the 50th jubilee?
Sister Helen's missions:
St. Aloysius, West Allis, Wis.
St. Joseph, Escanaba, Mich.
St. Peter, Beaver Dam, Wis.
Holy Name, Sheboygan, Wis.
St. Joseph, Black River, Falls, Wis.
St. Nicholas, Freedom, Wis.
St. Stanislaus, Berlin, Wis.
Mother of Perpetual Help, Milwaukee, Wis.
Holy Trinity, Oconto, Wis.
St. Louis School, Dykesville, Wis.
Our Lady of Mount Carmel, Mount Calvary, Wis.
How to Reach Jubilarians
Cards and letters may be addressed to:
Sister's name c/o SSND Jubilarians, Provincial Offices, 13105 Watertown Plank Road, Elm Grove, WI 53122-2291.
If you would like to honor sister with a tribute gift, please click HERE.
Return to jubilarians' main page


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Bishop T.D. Jakes is more than excited about his upcoming MegaFest event in Dallas, Texas, on August 29-31. According to Jakes, in addition to the concerts, balls, and a Lifeclass with Oprah, the event will tackle some key issues in our community.
"We're going to tackle some tough subjects," said Jakes. "Get down to some things that are cutting at the underbelly of our community. Whether it's with a Lifeclass or whether it's with some of the workshops or town-hall meetings... we're going to grapple with some issues."
Check out the interview above.