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What Should Jerry Reese Do?
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Joined: 23 Feb 2012
Posts: 3964
Location: Northeastern PA
Posted: Fri Jan 04, 2013 2:34 pm
Post subject: What Should Jerry Reese Do?
Take a look at the different positions and say what you think the Giants should do.
Coaching- TC will be back. Unfortunetly, Gilbride, who was once rumored as a BC HC canidate, will be back. I don't think Fewell will be let go either. But maybe bring in someone with same unique ideas for defensive and offensive. Defensive had a lot of injuries, but Offensive was too vanilla.
QB- Eli is still Eli-te to me. Carr should be resigned as backup. Bring a developmental QB (Jeff Tuel?) for the Practice Squad.
RB- Earth (Brown), Wind (Bradshaw), and Fire (Wilson) 2.0 will be back, but Reese should bring in a couple vets in case 1 or more gets injured. Dare we say Brandon Jacobs? Kregg Lumpkin is reliable at least. Hynoski is entrenched at FB.
WR- I believe Randle is ready to step up full-time in our 3 WR sets. Nicks needs health. Cruz needs to stop dropping. Hixon should be back at a reasonable price as depth WR/ST. Add in another rookie, an UDFA like Chad Bumphis) and a KR/PR whether its Jernigan or a FA.
TE- Robinson will hopefully be ready after a full year to give us a 2nd good TE, but I still believe Bennett should be resigned. There is little FA depth or we would have to use another draft pick to get a replacement. Bear can wear many hats so he still has value.
C/G- Do we cut Baas? I say we do if/when we can ensure we have a reliable replacement. We don't have to do it yet. Boothe should have a reasonable price tag and be back. Snee is still the best of the bunch. Brandon Mosley could be looked at G to either replace Snee in the future or Boothe with him sliding down. Any extra picks we get can be used for intirior OL depth and sign a cheaper placeholder OG like "Butt-Fumble" Brandon Moore. Spending $6 million+/year on a FA guard never works out.
OT- Priority #1 is resign Will Beatty. He has finally become the LT we need. Priority #2 is cut Diehl right away. An OL needs continuity as much as talent. Use our 2nd Rnd pick on an OT (Dallas Thomas) and sign a reasonable FA. I like Will Svitek.
DT- Linval Joseph is 1 starter. Canty should be back if he's willing to take a pay cut. He can still make an impact when healthy, but isn't worth his current salary. Draft a DT in the mid-rnds. Have Kuhn and Ausitn fight for a spot. Sign a FA too. Richard Seymour has been suggested. Sounds good to me.
DE- JPP is a beast and can play either DE position. Tuck, I believe, would take a pay cut down to $3 million or so. Osi is gone. He played great, but its time to move on. We then use an Day 1-2 pick on a DE (Dantone Jones in Rnd 3 for me). Moving Kiwi to DE permanently would give us more depth and bring new blood to our LB core.
LB- There is no questioning, our starting LB core, Chase, Kiwi, and Boley is probably the worst in the league. It needs an overhaul. Kiwi is moved to DE. His replacement could be a FA. A reasonably priced FA like Brad Jones could fit well as a SAM LB. Chase should be resigned as a FA, but for depth purposes and ST ace. His replacement at MIKE could be Boley (if we don't cut him) or a rookie (Alex Ogletree or Khaseem Green a little earlier, Gerald Hodges a little later). We can ask Boley for a pay cut. Rivers is hurt and not very effective. He's a FA and can move on. It's JWill's time for a permanent starting role at WILL.
SAF- Kenny Phillips has been great, but his price tag with his injuries make him to expensive for a high-end long-term deal he will get elsewhere. Trel will be back at 1 spot. I recommend splurging a bit for William Moore, a really good combo safety that fits our scheme and is on a team that just resigned a SAF last year to big money and have 2 big money CBs. Stevie Brown and Will Hill has proved to provide excellent depth and ST. IMHO, I can't see Brown repeating his success, which is why I'm leary of relying on him. He's been a ST Journeyman. No question he was great this year, but now teams will have film on in the offseason. Hill probably needs another year after being away from the game so long. He can replace Trel in 2014.
CB- Prince established himself as 1 starter, but another is needed. Hosley is best suited for slot duty. Using a 1st Round pick (Jonathan Banks, Xavier Rhodes, or Jordan Pyor) would give us a #2. Webster could be given a pay cut to $1.5-2 mil. Say no, then your cut. TT2 is gone with his option not picked up. Adding a 2nd CB through the draft (Honey Badger?) or another 1-2 in FA gives us depth. Marcus Trufant sounds good.
ST- Weatherford and DeOssie are safe. Tynes is a FA. I say draft Dustin Hopkins in Rnd 6, save $2 mil, and don't look back. With Wilson and Randle stepping into offensive roles, we could use a KR/PR. Jernigan hasn't proven anything, so a FA (Bengals's Brandon Tate) or a late Rnd pick/UDFA like O.J. McDuffie could be a replacement.
What do you think Jerry Reese should do at each or a certain position?
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Joined: 14 Jan 2007
Posts: 12126
Location: USA
Posted: Fri Jan 04, 2013 3:36 pm
Post subject:
RB: Do not think that Bradshaw is a guarantee to come back. He is making nearly 4 million and that is a lot to pay for a mostly injured backup running back.
Jacobs is done, he is not rejoining the Giants
Brown is a Exclusive Rights FA so he should be back
For the OL we need to sign or draft a OG or C and hopefully Brewer can win the RT spot. We need to resign Beatty badly
DT: We need Canty to reduce his salary and we need to draft another one
LB: Boley WILL be cut, he barely played our last few games, our LBs are garbage and we need a new one at MLB and SLB. Jwill can play the WLB
CB: Webster whole entire salary can be turned into a bonus which will reduce his capt hit.
S: With Phillips probably gone even we need to draft one badly. There is talk with T2 moving here if he can recover from his 3rd ACL injury.
In conclusion our draft better be BPA at
of the defensive positions and we sign a starting caliber OL.
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Joined: 15 Mar 2007
Posts: 4062
Location: Giants fan in the Seahawk's backyard...
Posted: Fri Jan 04, 2013 3:47 pm
Post subject:
in my estimation this will not be a particularly popular idea, but (and i'm assuming that Chance Warmack goes to Dallas with the pick prior to us) the "smartest" pick that we might make would be taking Barret Jones. His versatility on the OL is basically unmatched in this draft. he would be the eventual replacement for Baas/Boothe, but can play any position on the line (former All-SEC left tackle).
i know it won't be the "popular" choice, but its a smart one.
I feel sorry for people who dont drink. They wake up in the morning and thats the best theyre going to feel all day.
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Joined: 11 Apr 2012
Posts: 158
Location: ATL
Posted: Fri Jan 04, 2013 3:56 pm
Post subject:
QB- Yep
QB EDIT: We are signing Curtis Painter, so David Carr's time may be coming to an end.
RB-Yep but I think Bradshaw should be the fire and Wilson should be the Wind.
I think we should look at the very late rounds for another runningback. I am looking at Zac Stacy, Vanderbilt, and if we are lucky, maybe from the trade down we can do that some people want we can try Marcus Lattimore, South Carolina if he drops far enough.
WR- Yea agree with everything, definitely need to try and get Hixon for at least another year. Get another WR undrafted or through free agency. I don't like the way Nicks' health is acting.
Note: If we want to try something new in our offense we can kill two-three birds with 1 stone sorta and draft Dri Archer. He is projected to run high 4.20s or low 4.30s, and can play RB, WR, KR, PR. I have always wanted a Darren Sproles kinda guy. But Unlikely sadly.
TE- Resign Bennett, Adrien Robinson is supposed to be the "JPP of TEs" so this spot should be good.
C/G- Snee is staying cause of family. Boothe should be resigned. Baas I think we are stuck with for now.
OT- Will Beatty resign. Diehl cut. Draft someone in 1st or 2nd round. Im thinking also about Dallas Thomas. Or we can take a risk and go for the Wisconsin Rick Wagner.
OT EDIT: Locklear gets resigned also. Also something interesting about Locklear:
- Only OT with 500+ snaps that didn't lettup a sack.
DT- Linval Joseph is good. Canty will take a pay cut most likely. I am interested in what Kuhn is all about. Austin probably get cut during preseason next season. I like Sheldon Richardson in the 1st round but that conflicts with us getting Johnathan Banks. Seymour I don't want because age, injuries, may ask for alot of money, leadership problems. If we go through free agency for a DT though a player we could look at could be Justin Bannan. He won't cost as much.
DE- My favorite. JPP is getting lessons from Strahan this offseason hopefully so he will probably explode next season. Tuck needs a paycut. Osi is gone like you said. You say Dantone Jones in Round 3. Well I say Devin Taylor in Round 4/5. The thing is if we are even thinking about releasing Osi, that must mean we are confident in Adrian Tracy and Ojomo. Plus I just love Devin Taylor for some reason. He is 6-7, 270 and was one of South Carolina's best DEs if not the best until that beast of a man Clowney came.
LB- Chase was our best LB this season, that is saying something. Jacquian Williams has WILL pretty locked for the future. Keep Blackburn for one more year. I am not a real fan of Ogletree from Georgia but everyone hear likes him, so who knows. There are some good LBs coming out in free agency so we can look there. Kiwi can move to DE so that just adds to the point that we don't need to spend too high of a pick for DE. But Reese loves him some DEs so i am not sure. I think we need to find some athletic LBers in draft I like are ofcourse Te'o, Jones, Arthur Brown, and Khasheem Greene. I am hearing alot of good stuff about Andrew Jackson, from Western Kentucky though, but don't know if he is declaring.
SAF- Kenny Phillips should be resigned, but will probably be given a better offer by another team because we might need to pay cut him. Antrel Rolle is fine for now. Brown was a nice pickup. Will Hill has potential. I want Cooper Taylor, S, Richmond as undrafted pickup though. Adds depth, was once best SS for Georgia Tech, familiar with 3 safety set, tall, etc. However he has Wolff-Parkinson-White Syndrome. Also we have Tyler Sash, forgot to mention. We are good at safety.
CB- Prince is good he just needs a partner. Cut Terell Thomas or a very low paycut. Cut Corey Webster's pay aswell. Draft Johnathon Banks or Jordan Poyer in 1st rounds, Or go with sleepers B.W. Webb from William & Mary(nickel corner though) or Sanders Commings from Georgia. When it comes to cornerbacks nowadays I marvel at Seattle. Their cornerbacks are both over 6'2 and get the job done. So I would prefer Johnathan Banks and Sanders Commings.
ST- This spot is good. However we should be looking for a new kicker. Dustin Hopkins would work, and for returning I point you at Dri Archer.
Thats what I got to say. Good stuff.
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Joined: 23 Feb 2012
Posts: 3964
Location: Northeastern PA
Posted: Sat Jan 05, 2013 1:47 am
Post subject:
I checked on Archer. He is a Junior, and at his size is a Day 3 pick, so maybe next year. Put in Theo Riddick in my mock offseason. He is bigger and not as fast as Sproles, but was a great all-purpose back/WR/KR at ND so he gives us some options.
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Joined: 20 Nov 2007
Posts: 1381
Posted: Sat Jan 05, 2013 2:22 am
Post subject:
dkebones wrote:
in my estimation this will not be a particularly popular idea, but (and i'm assuming that Chance Warmack goes to Dallas with the pick prior to us) the "smartest" pick that we might make would be taking Barret Jones. His versatility on the OL is basically unmatched in this draft. he would be the eventual replacement for Baas/Boothe, but can play any position on the line (former All-SEC left tackle).
i know it won't be the "popular" choice, but its a smart one.
I 100% agree. Not at 19 though (atm). We need to move back. I can't see him blowing up the combine but he has giants written all over him.
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Joined: 07 Sep 2009
Posts: 28865
Location: Founder and Chairman of the He-Man Oline Haters Club.
Posted: Sat Jan 05, 2013 4:27 am
Post subject:
I see very few problems with the coaching staff and more of the players under performing.
Bringing back Jacobs is a horrible idea, with his time in SF I've come to realize he is just a cancer waiting to be poked at and should just be left alone.
Randle at 3rd will be good, Cruz will have less targets because of it which in turn will limit his drops.
Hopefully Bennett will resign because at a TE position he's what we need right now. He also know's our offense and teaching another TE will just be a waste of a year.
Our O-Line will be shaped by the draft, depending on who TC drafts they will most likely start a few games.
Our D-Line should return to its true form, wouldn't be surprised in a 5/6th round pick DT. Tuck and Jpp should be able to man down the line, and hopefully Kiwi is put on permanent D-line (which I highly doubt will happen)
On the Lb situation Boley stays, along with Chase. Kiwi hopefully gets moved down to our D-Line and depending on the draft Ogletree should sub in for him.
Not sure on the Saftey/CB positions, I'm positive Brown and Prince will be starting. With Websters "decline" he looks to be gone (Traded). Hopefully
Thomas can come back from his injury, I highly doubt he can. But before he got hurt returning from his first injury he looked like the good ole TT.
I believe our ST guys are safe (Tynes/Weatherford ), but wouldnt be shocked if we drafted a Kicker
Can't spell Gen without Glen
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Joined: 19 Jan 2009
Posts: 10897
Posted: Sat Jan 05, 2013 12:23 pm
Post subject:
Hopefully we get Kiwi back at DE. If I remember correctly he played much better there than at LB. He's also on the record saying he likes playing DE much better...so I'd love some quality LB falling to us at some point.
Hopefully we can get an O lineman at some point to replace Diehl. That's the only big problem we have with the line IMO.
I don't really have any issues with the coaches...but that's usually my stance 'round here
I just wish Killdrive would turn the offense over to Eli more often and run a no-huddle similar to what the Pats do.
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Aaron "CB" Thompson
82, of Tucson passed away April 5, 2013. Services will be held Saturday, April 13, 2013 at 11:00 a.m., EVERGREEN MORTUARY, N. Oracle & W. Miracle Mile. Viewing will be held at 10:00 a.m. until time of service. Please see www.evergreenmortuary-cemetery.com for full obituary and guest book.

subset: commoncrawl


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Suddenly, the three most notorious assassinations in Russia are solved, and the masterminds are the very ones President Vladimir Putin suspected all along. Forgive us if we remain skeptical.
Russia and the Usual Suspects
Current Status: Blessed (1)
Seeded on Mon Sep 3, 2007 12:27 PM
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At the beginning of Season 11, it was shown that hoodies/jackets no longer had to have a Degrassi logo on it, and it could be of the students' choice. Extras (jewelry, headbands, scarfs) could also be worn. Head coverings were also supposedly banned as well, as a number of students who had usually or always worn hats/beanies in school under the prior rules (K.C., Adam) no longer did so.
While it was practically unheard of for a real-life school that's never had uniforms to implement them at any other time than the start of a new school year (with ample prior notice the preceding spring), several other teen shows have done so in midyear as a one-episode storyline.
At the beginning of Season 12, the dress code was loosened, and students no longer had to wear uniforms and got to wear the clothing of their choice. Students are still required to display student ID on lanyards. Starting with the first episode of Season 15, the student IDs could be worn on a lanyard or clipped onto a student's backpack.
Colors for the polo shirts
- Seniors (Grade 12) Blue
- Juniors (Grade 11) Red
- Sophomores (Grade 10) Purple
- Freshmen (Grade 9) Yellow
The first publicity photos of the second half of Season 11 show incoming Grade 9s wearing yellow shirts in the next school year, meaning the students promoted to the next grade will be obliged to buy different coloured shirts every year.
This does confirm that Grade 7 and 8 students no longer attend Degrassi and are not just merely out of focus.
As of Come As You Are (1), the uniform policy has been abolished, while the requirement to display student ID remains.
The plot of Got Your Money (1), is set in motion by the fact that Zig still wears khakis bought for the now-obsolete uniform.
In the opening credits
Although they had been introduced as students returned after midwinter break partway through Season 10, the uniform-free introduction continued to be used for the remainder of the season. Likewise, Season 11's credits carried over Grade 10-12 students' old pictures with their last year's shirt colors after the start of the new school year. With the change in policy in Season 12 coming in the cold open of the first episode, uniforms were dropped from the intro with immediate effect.


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text:
High Altitude Observatory staff traveled to Chile and New Mexico last month for a classic HAO event: observing and documenting a total solar eclipse. This one passed over Putre, Chile, where several minutes of totality on 3 November gave Kim Streander, Dick White, David Elmore, Alice Lecinski, and Greg Card an opportunity to record film and digital images from three coronal cameras. (Other cameras were trained on the researchers themselves. The Cable News Network filed a report from Chile, and British and local media were also on hand.) Some cirrus was present over Putre, but the group hopes that analysis will show the eclipse images were not significantly hurt. The eclipse coincided with the "dia de todos los Santos" (All Saints' Day) celebration and a regional fair, producing a mix of "llamas, tourists, Native Americans, and scientists," as Dick and Kim put it. Their summary after three hectic days of instrument placement, calibration, and data collection: "Everyone is tired. Packing. Like Chile! Sell condo, car, etc. Send proceeds! Enlisting in Chilean army."
The Chilean effort was complemented by the third launch of the Woods rocket, managed by HAO's Tom Woods. The NASA rocket left White Sands Missile Range on the day of the eclipse bearing several new x-ray and ultraviolet imagers. Full-disk solar irradiance values and coronal images were taken at wavelengths of 17.5, 30.4, and 121.6 nanometers (the primary emission wavelengths for iron, helium, and hydrogen in the solar corona) to supplement the Chilean data. The project's other goal, in conjunction with the University of Colorado's Laboratory for Atmospheric and Space Physics, is to study variability of the solar extreme ultraviolet irradiance and its influence on the upper atmosphere. HAO investigators include Tom, Don Hassler, Tom Holzer, Gary Rottman, and graduate student John Worden. Engineers Greg Ucker and Ray Wrigley worked with the NCAR machine shop to fabricate the new imagers.
This photo of the solar corona was taken during the total eclipse at Putre, Chile, at 12:18 Greenwich mean time on 3 November. The Newkirk camera system photographed the corona in red light through a filter that suppressed the bright inner corona in order to show the much fainter streamers of the outer corona at the same time. Because the photograph was taken late in the eclipse, prominences on the east limb are visible. (For this and for solar images in general, north is at the top, east at left, and west at right.) Other features of note are the polar plumes and the nonradial nature of the southwestern streamer. A coronal mass ejection was observed in the vicinity of this streamer by NCAR's Mauna Loa Solar Observatory about ten hours after the eclipse. (Photo courtesy Alice Lecinski and HAO.)
Though a number of people who wished to express their high regard for Phil were unable to attend in person, their thoughts were included in written testimonials to Phil. For example, long-time friend Aksel Wiin-Nielsen (assistant director of NCAR's Laboratory for Atmospheric Sciences at the time Phil was its director) could not attend. However, Joe Tribbia delivered Aksel's scheduled speech, which addressed Phil's long-term scientific and adminstrative achievements and afforded a personal glimpse of Phil's warm and intelligent personality. Mel Shapiro (NOAA) read a letter from Arnt Eliassen, a long-time colleague now based in Norway, who noted that Phil's scientific intuition "was very much guided by his strong aesthetic sense. He demanded from scientific theory that it should be beautiful and elegant." Eliassen also cited Phil's "wonderful command of the English language." Speakers present included Earl Droessler (North Carolina State University), Joseph Fletcher (NOAA), Will Kellogg (NCAR), Chuck Leith (Lawrence Livermore National Laboratory), Doug Lilly (University of Oklahoma), Gerald North (Texas A&M University), and WarrenWashington (NCAR).
According to Joe, "The symposium succeeded in honoring Phil's accomplishments in science and his leadership role at the Institute for Advanced Studies, the Air Force Cambridge Research Lab, the Joint Numerical Weather Prediction Unit, and NCAR, in a manner in which Phil would approve--discussing the scientific merit of these activities."
Among the more personal reminiscences shared was the influence of Phil's father, a botanist at the University of Illinois. Chester Newton (NCAR) noted that other faculty from Illinois would visit Phil's boyhood home bearing puzzles and quizzes, which Phil loved. "Phil was also a historian of science," recalls NCAR archivist Diane Rabson, who recorded the symposium for posterity. "He was active in our oral history projects and served on the NCAR history committee."


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Robert P. Myers
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Rob Myers was born in Pascagoula, Mississippi on August 21, 1966. He graduated from Pascagoula High School in 1984 and then attended Mississippi State University where he received his Bachelors of Professional Accounting in 1988. He received his Juris Doctorate from the University of Mississippi in 1991.
While at Ole Miss., Rob served as member and Treasurer of Moot Court Board in 1990-1991 and was a member of Phi Delta Phi legal fraternity.
Rob Myers was admitted to the Mississippi Bar in 1991 and is licensed to practice in various courts in Mississippi including the United States District Court, Northern and Southern Divisions, the Mississippi Supreme Court, and the Mississippi Court of Appeals, as well as the United States Fifth Circuit Court of Appeals.
Upon graduating from law school in 1991, Rob practiced law with the Gulfport firm of Mize, Blass, Lenoir & Laird, which later merged with the firm of Aultman, Tyner, McNeese, Ruffin & Laird.
In May 1995, Rob joined the firm of Owen & Galloway, P.L.L.C. where he practices law today. Rob became a partner in the firm on January 1, 2000, and his main areas of practice are medical malpractice, personal injury, and probate.
Rob served as a member of the Board of Directors for the Mississippi Young Lawyers' Association in 1996 and served as President of the Harrison County Young Lawyers Association in 2000.
|University of Mississippi School of Law
|Law School
|Mississippi State University
|State / Court
|U.S. District Court Southern District of Mississippi 1991
|U.S. District Court Northern District of Mississippi
|U.S. Court of Appeals 5th Circuit 1991
|Mississippi 1991
Additional Info
Service Type:Private
1414 25th Avenue
Gulfport, MS 39501


text:
Do You Still Link Like Its 2005?
You come across a site promising 500 do-follow links for $50. Wow what a good deal you tell yourself. You'll be number one on Google in no time. Ha! If only this were true. You're still building links like its 2005, and need to reevaluate your game plan.
While traditional link building may still work for some, the trend has been and will continue to lean toward content. Good content, when publicized correctly, will naturally receive traffic, links, and translate into better rankings for your site overall.
Tags: Content Development, facebook, Guest Blogging, Link Building, linkability
About Kaila Strong
As Senior Director of SEO Services, Kaila oversees both the SEO department and our Internet Marketing Strategists. She works with our expert team to uncover SEO strategies, develop link building campaigns, conduct competitive analysis, review Google penalties, execute backlink analysis and train peers on SEO fundamentals. +Kaila Strong


text:
On her fan club site, the 'Cowboy Casanova' singer writes about the time she pulled over to help two puppies on a median of an interstate highway between Oklahoma and Tennessee.
Carrie Underwood apparently deserves the title of stray savior. The "Jesus, Take the Wheel" hitmaker has shared the story of how she took part in saving the life of an abandoned dog over the holidays, writing on her fan club site that she pulled over to rescue the injured pup and found it a permanent home.
In a December 30 post, the 28-year-old singer recalled the time she saw two dogs on the median of an interstate highway between Oklahoma and Tennessee as she drove home from a visit with her parents. "I had Ace and Penny [her own dogs] and a trunk full of luggage and presents in the SUV," she wrote.
Concerned for the pups' safety, the wife of NHL star Mike Fisher pulled over only to find out that one of them had died. "My guess is that the dogs were thrown out of their car by their owners on the highway," she noted. When the other pup that she named Stella started wagging her tail, she picked it up, put it into her car and took it to her emergency vet.
On New Year's day, Underwood brought up another post about Stella. The season 4 winner of "American Idol" revealed that Stella was getting house trained, recovering from her road rash and getting her teeth cleaned. She added, "She follows me around from room to room. She's the best Lab I've ever been around!"
A day later, Underwood informed her fans that Stella has been adopted by some neighbors down the street. Although her neighbors told her that she could visit now and then, the country singer wrote, "I cried when she walked out the door. [Fisher] told me not to be a crazy ex-girlfriend about it and stalk them ... I can't help it! She deserves the best life ever!"


text:
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Translations into English
|Named Person:
|Czesław Miłosz; Czesław Miłosz
|Document Type:
|All Authors / Contributors:
Czesław Miłosz
|0060745665 9780060745660
|OCLC Number:
|102 pages ; 24 cm
|Second space --
Late ripeness --
If there is no God --
In Krakow --
Framing --
Werki --
Advantage --
A master of my craft --
A stay --
On old women --
Classmate --
Tenant --
Guardian angel --
A beautiful stranger --
To spite nature --
I should now --
High terraces --
Nonadaptation --
Hear me --
Scientists --
Merchants --
Coffer --
Degradation --
New age --
Eyes --
Notebook --
Many-tiered man --
Father Severinus --
Treatise on theology --Apprentice --
Orpheus and Eurydice.
|Other Titles:
|Czesław Miłosz ; translated by the author and Robert Hass.
|More information:
"Nobel Laureate Czeslaw Milosz's most recent collection Second Space marks a new stage in one of the great poetic pilgrimages of our time. Few poets have inhabited the land of old age as long or energetically as Milosz, for whom this territory holds both openings and closings, affirmations as well as losses. 'Not soon, as late as the approach of my ninetieth year, / I felt a door opening in me and I entered / the clarity of early morning, ' he writes in 'Late Ripeness.' Elsewhere he laments the loss of his voracious vision - 'My wondrously quick eyes, you saw many things, / Lands and cities, islands and oceans'--only to discover a new light that defies the limits of physical sight: 'Without eyes, my gaze is fixed on one bright point, / That grows large and takes me in.' Second Space is typically capacious in the range of voices, forms, and subjects it embraces. It moves seamlessly from dramatic monologues to theological treatises, from philosophy and history to epigrams, elegies, and metaphysical meditations. It is unified by Milosz's ongoing quest to find the bond linking the things of this world with the order of a 'second space, ' shaped not by necessity, but grace. Second Space invites us to accompany a self-proclaimed 'apprentice' on this extraordinary quest. In 'Treatise on Theology, ' Milosz calls himself 'a one day's master.' He is, of course, far more than this. Second Space reveals an artist peerless both in his capacity to confront the world's suffering and in his eagerness to embrace its joys: 'Sun. And sky. And in the sky white clouds. / Only now everything cried to him: Eurydice! / How will I live without you, my consoling one! / But there was a fragrant scent of herbs, the low humming of bees, / And he fell asleep with his cheek on the sun-warmed earth.'"--Jacket.
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by dsimonse updated 2011-08-17


text:
The Critical Path is a series of very brief interviews with famous game creators on all manner of heady topics related to game design.
There's Clint Hocking discussing how games convey loss, Tim Schafer on the economy of writing being especially important in interactive media, and Hideo Kojima on placing messages about social issues in games. Regrettably, Kojima doesn't debate the economy of writing with Schafer. At least not yet.
The web interviews are just a start, though, as The Critical path is actually a multi-format "transmedia project exploring the art, philosophy, politics and psychology of video games" that will become a full-length documentary, as well as a mobile app.
Creator Artifact Studios' goal is to "give game designers their due as innovators and influencers of culture."
This project has been in the works for two years. Artifact Studios noted that user feedback will dictate the sort of content to come.
Check out the trailer below, or just go directly to the Critical Path site and check out some micro-interviews as they're even shorter than the trailer.


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The IPN Photography Agency for Photographers & Trade has announced its upcoming event "Photofest Ireland." Taking place on 28 April 2013, this one-day event will be held in the Crowne Plaza Hotel and Conference Centre in Santry, a suburb on the Northside of Dublin, from 10am - 6pm. The programme includes an array of seminars, product demonstrations, and more. Entry is free but advance registration is advised. You can pre-register at the website below.
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Tag Archives: Florida Stage
Nan Barnett Takes Exec Director Post At Atlanta's Actor's Express Theatre
Nancy Barnett, the former managing director of Florida Stage, has taken a job as executive director of Actors' Express Theatre in Atlanta effective immediately.
StageBill Blog: Looking Back At The Year of the Sea Change
In real life, we rarely have the clarity of identifiable watersheds as heroes discover in dramas. But five years from now, you'll likely look back on the past 12 months and recognize not a turning point, but an unmistakable moment within a slow sea change in South Florida theater.
Nan Barnett's Essay on the Death of Florida Stage and the Personal Fallout
Nan Barnett, managing director of Florida Stage, has written a stunning essay for American Voices New Play Institute, revealing both the devastating and transformative effect the closure had on her life as well as the first extensive insider's account of how it occurred.
Looking Back: The Best (and Worst) of the 2010-2011 Season
By Bill Hirschman (CORRECTED 9:47 A.M.) Theater reviewers must have a better than average ego to flatter themselves into believing that their judgment has worth, regardless of what anyone else thinks. So imagine critics asking each other for a reality …


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About the Author
Maria Poblet
Raised in Buenos Aires, politicized in East Los Angeles, Maria Poblet is a nerdy Latina rooted in the San Francisco Bay Area. Building off a decade of radical community organizing and movement building work, she lead the merger of the Latino organization she built with a Black organization, forming a single, multi-racial powerhouse called Causa Justa :: Just Cause (www.cjjc.org). Before organizing, she was Artistic Director of Poetry for the People, and had the honor of being mentored by June Jordan. Follow her on twitter @mariadelpueblo
Maria Poblet Tags
amilcar cabral BART Strike Catalyst Project causa justa classconsciousness class suicide community organizing displacement feminism gentrification google bus googlebus grassroots global justice housing crisis Internationalist Feminism labor organizing mariadelpueblo maria poblet mission district oakland privilege public sector workers revolutionary democracy san francisco social movements tech techies tenant organizing World March of Women world social forum tunisia
Recent Maria Poblet Posts
- The Struggle for the Flatlands: How Oakland Can Fight Gentrification Written on Tuesday, 22 April 2014 06:39
- Revolutionary Democracy, Class-Consciousness, and Cross-Class Movement Building: Lessons from Amílcar Cabral Written on Wednesday, 19 February 2014 19:51
- Gentrification and The Battle for the Heart of San Francisco Written on Tuesday, 04 February 2014 07:14
- The BART Strike, and Beyond Written on Tuesday, 22 October 2013 05:23
- Until Every One of Us is Free: Reclaiming Feminism at the Grassroots Written on Thursday, 03 October 2013 00:49


text: On Fri, 26.10.07 09:29, Kelly Miller (lightsolphoenix gmail com) wrote: Hi! >> It will tell you which processes have opened your audio devices > Yeah, I managed to find the cause; PulseAudio was trying to grab the audio > device twice, and the error came from the second attempt. When I corrected > the error in the configuration file, those errors went away. So you modified PA's config file, right? The default config file should work fine for most people. So if I understand correctly this was not fault at all on PA's side, but just a configuration error on your side? > However, I can confirm that there's some kind of memory leak problem in the > ESound protocol handling, which I narrowed down to the do_read method via > tracking the error message I got in the source. As I mentioned before, > sometimes PulseAudio will jump to 50%+ memory used and then shut itself > off; when I ran it using pulseaudio -vv, the error I got lines up with the > do_read function's general error messages. I can get a log of that if > necessary as well. Yes, please! Most helpful would be some kind of memory trace. Best would be an output of valgrind's massif tool. Thank you, Lennart -- Lennart Poettering Red Hat, Inc. lennart [at] poettering [dot] net ICQ# 11060553 http://0pointer.net/lennart/ GnuPG 0x1A015CC4


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Samsung Electronics's debut of its Galaxy 10.1 tablet computer in Australia may be delayed beyond the end of the month after a judge said she needs time to study Apple's patent-infringement claims.
Federal Court Justice Annabelle Bennett may grant a "brief" injunction on sales of the Samsung tablet as she considers arguments from Samsung and the iPad maker, the judge said yesterday during the first day of a two-day hearing over whether the Galaxy Tab should be banned in Australia until the dispute is resolved. The judge urged both sides to consider starting their patent-infringement trial as soon as possible.
"Technology moves very quickly, " Bennett said. "It would be in both sides' interest to have this matter finalised quickly."
The dispute is part of a global fight between the two companies, the world's two largest makers of tablet computers, which began in April in the U.S. after Cupertino, California- based Apple sued Samsung claiming Galaxy products "slavishly" imitated the designs and technologies used in iPads and iPhones. Samsung struck back with lawsuits in South Korea, Japan, Germany and Australia.
Samsung targeted the iPad 2 and adopted as many of its features as it could in developing its newest tablet, Steven Burley, Apple's lawyer, said today.
It must have been "as plain as the Opera House to Samsung" that the design of the Galaxy Tab 10.1 infringed Apple patents, Burley said. "They ought to clear the way in advance rather than attempt to crash through."
'High stakes'
Samsung threatens to steal Apple's sales of iPads with its products and that would also threaten sales of iPhones, applications and accessories because people like to stick with one brand, Burley said.
A ban on the sale of the product would go too far, David Catterns, Samsung's lawyer, told the judge.
"This is a high-stakes fight," Catterns said. "This is where we draw the line."
Apple's iPad may account for 73 per cent of tablet computer sales this year, according to the research firm Gartner. Products that run on Google Inc.'s Android operating system, including Samsung's Galaxy tablets, will probably have about 17 per cent of the market, Gartner said in a Sept. 22 note.
Samsung agreed in August to delay a planned release of the Galaxy 10.1 tablets in Australia until Bennett rules on Apple's request for an injunction barring the sale of the product.
Fighting until iPad 9
Under usual circumstances it would take "well into next year" to resolve the patent dispute, Catterns said. Apple would be prepared to go to trial next week, Burley told the judge.
Catterns said that by the time the patent dispute is resolved at trial, Apple would be selling the iPad 9.
Samsung had altered its Galaxy 10.1 tablet from a U.S. version that Apple claimed infringed 10 of its patents, for release in Australia. The Australian version, although with "reduced functionality," still infringes at least three patents, according Burley. Catterns denied the Australian tablet has reduced functionality.
Apple didn't object to earlier versions of Samsung's tablets even though they used the same technology, Catterns said.
The 10.1 is a bigger threat to Apple because it's the thinnest tablet computer on the market, Catterns said.
"They didn't sue before but now they're worried," Catterns said of Apple. "It's the thinness of our product that worries them."
Zoom technology
Samsung countersued Apple on Sept. 17, saying the iPhone and iPad infringe seven of its patents related to wireless communications standards.
At yesterday's hearing, Apple focused on one alleged patent infringement, relating to the touch screen technology of the iPads. Samsung had agreed not to fight Apple's claim that the Galaxy 10.1 uses zoom technology that infringes its patent.
The agreement to halt advertising and the sale of the Galaxy 10.1 tablet doesn't affect any other Samsung tablet or smartphone available in Australia, or other countries, the company said following the Aug. 2 hearing.
A German judge barred the sale of the Galaxy 10.1 tablets in that country on Sept. 9, pending a trial of Apple's patent claims. Apple also won an injunction in Germany prohibiting the sale of the Galaxy Tab 7.7, which has a smaller screen than the 10.1 or the iPad. That forced Samsung to pull the product from the IFA consumer-electronics show in Berlin earlier this month.


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I make duct tape (with Duck tape) bags, various types of jewelry in all kinds of styles, and my newest love is drawing, particularly with Copic Sketch markers! As of June 2013, I've been using Copics for about 3 months.
I just turned 44 years "old" and feel younger than I have in a long time (: Which is seriously weird, since my daughter just turned 18.... I dunno how that happened! Where'd all that time go?!? She's actually been my inspiration to delve into the drawing world, because she's been doing it since she was a toddler. I still remember the look of wonder on her face, the first time she drew a crayon across a page and couldn't believe the color appeared there I love her drawings so much, I got one of them tattooed on my leg.
My other inspiration is my best friend, who got into zentangling last summer (2012.) She's fabulous at it. I did some doodling myself for a while, but I think I've realised that I like doing slightly more realistic/themed drawings and have left doodling by the wayside.
Other things I love.... reading! I do it all the time. I read pretty much every type of fiction, but sci-fi/fantasy are my top loves, followed closely by thriller/mystery type stuff. My library room has overflowed into the rest of my house, and I own about 4000 books. Yes, I have read them all. I also love to cook and create my own recipes, but I am NOT a baker (; I've travelled all over the world and am certainly not done with it - if only it weren't so darn expensive!
When I'm not reading, drawing, cooking or watching TV, I'm gaming on my PC. All time love is Mass Effect, and I've played the series 20+ times. In fact I'm replaying again right now, so I think I'll get back to that......


text:
< Back to Faculty Services
Media Booking
In addition to the media located in the Library collection, Palomar faculty may borrow videos and DVDs from the San Diego Office of Education (SDCOE) Consortium collection of over 12,000 titles. Faculty may also request videos be purchased for this collection that would not ordinarily be available from the Library.
Quick Link: Consortium Catalog
There are no charges for booking media. Delivery is on Tuesday or Friday. Items are delivered to your department office or off-campus site, unless other arrangements are made. Media cannot be delivered directly to the classroom.
Choose One Of The Following Methods To Borrow Videos or DVDs:
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text:
- Description
This is a super dress made from chiffon fabric with a bold abstract print in green, yellow, blue and black. It has short sheer sleeves and a wrap over bodice forming a V neckline. The waist is seamed and there is a faux wrap over panel to the front skirt. The dress is lined at the skirt in yellow silk acetate and it fastens with a back zip and there are shoulder pads. There is a black pvc buckled belt.
Vintage items rarely conform to modern day sizings and the UK size we give is only a guide. You are advised to check the measurements carefully to ensure a good fit.
bust: 32"
waist: 28"
hips: 36"
length: 36"
Approximately UK size: 10 but please check measurements
1980s / CONDITION: Very good - there are some marks to the lining, unseen when worn. There is visible wear and tear to the pvc covering of the belt.
Please refer to our grading and sizing guides.


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Reader: Best Thai in Denver Has One of the Worst Parking Situations
Rick and Noy Farrell opened Taste of Thailand twenty years ago this month. The restaurant has remained true to its goals -- authentic dishes made with fresh ingredients -- for the past two decades. But over that same period, dining habits have changed a lot (few people linger over lunch, for example), and so has the Englewood neighborhood where this Thai spot is located. See also: Taste of Thailand Turns Twenty with Fresh Spirit and "Beautiful Fish"
Don't plan on finding parking in front of Taste of Thailand.
Says Denver Dave:
Best Thai in Denver -- no question. We'd go more often but the parking situation is really grim.
Are there other restaurants you skip simply because parking is impossible? Post your nominations for the town's worst parking situations below.
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There is no question that the people of Hong Kong own Victoria Harbour. The Protection of the Harbour Ordinance makes that clear. But it has never been clear who is ultimately responsible for managing and safeguarding their most precious natural asset. This has been left to various government departments with administrative power over one thing or another. One result is that the rightful owners have had little say in the loss of half of it to reclamation over the years and the loss of access to the waterfront. Two months ago Chief Executive Leung Chun-ying endorsed the suggestion that the existing Harbourfront Commission, an advisory body that has succeeded in improving access, be replaced by a harbour authority to oversee management, planning, design and development. In his policy address last week, he was as good as his word, pledging to back the authority with financial support, understood to be in the billions of dollars.
This is what a growing public chorus, including this newspaper and the Society for the Protection of the Harbour, has been calling for over many years. First there will be a six-month public consultation beginning in March in which all stakeholders including district councils can have their say. But the Post has learned that Kai Tak, Tsim Sha Tsui and the northern shore of Hong Kong island around Quarry Bay and North Point are likely to be the first areas to be revamped by the new authority to enhance public access and enjoyment.
This is good news. The government still owns 70 per cent of the harbourfront, but the lack of a senior co-ordinating agency has had regrettable consequences. Apart from reckless reclamation, insensitive planning and development has made the shoreline increasingly inaccessible. Attempts at improvements have been frustrated by divided responsibility. As a result, Hong Kong has not yet learned how best to integrate the diverse elements of a living, working harbour with enhanced public access, amenity and enjoyment.
Government support for establishing the authority, however, does not yet mean that respect for the public interest can be taken for granted, or that the coming consultation is a mere formality. There remains the question of genuine public representation on the authority to ensure the right balance between government, commercial and public interests. Better still, the chief executive should give an unequivocal assurance on this point now.


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Delegates at the Royal College of Midwives annual conference in Cardiff want a reappraisal of the use of the three main obstetric interventions.
They called for expert bodies, including the National Institute for Clinical Excellence, to assess the evidence.
The conference heard that all three interventions can pose serious risks.
If women were given proper information on their use, the conference heard, they might make different choices that could have profound implications for the maternity services.
A reduction in caesareans and induced labour would help to reduce the number of births that require the use of instruments such as forceps - which are associated with a greater risk to both women and their babies.
Midwife Claire Wood, who put forward the motion, said: "Be very clear, this motion is not about setting professional against professional.
"Rather it could be an historical opportunity for all midwives to seize the initiative and lead the way forward in multi-disciplinary collaboration and to openly and honestly reappraise the care we give to all women."
Dame Karlene Davis, RCM general secretary, said: "Pregnancy, labour and childbirth is, for the majority of women, a natural healthy life event.
"We are concerned, however, that too many women are now receiving intervention, such as induction, epidural and caesarean section, without them always fully understanding the nature of these procedures and their potential risks.
"The RCM believes that the UK's caesarean rate is too high and will continue to campaign to inform women about all of their birth choices."
Guidelines from the National Institute for Clinical Excellence published last month urge doctors to persuade pregnant women not to have a caesarean if they do not need one.
The Department of Health supported the NICE guidelines, which are due to be reviewed in June 2005.
A spokesman for the department said: "We support the need for women to be offered evidence-based information and to enable them to make informed choices about their care during birth.
"Decisions about a woman's delivery need to be made by the woman in conjunction with the health professionals looking after her."
Around 1.5% of all births in England and Wales are caesareans carried out for non-medical reasons.


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Despite Berlin's reputed round-the-clock nightlife, all of the capital's bars close at some point, right? Well, not quite. Exberliner magazine's Jacob Heinze discovered four real 24-hour drinking joints...
The best of Berlin's 24-hour bars
Current Status: Blessed (1)
Seeded on Fri May 20, 2011 1:07 AM
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Home > Entertainment > People > Article
Former Hollywood madam Heidi Fleiss sobbed on the witness stand as she told a jury how her love affair with Blackhawk Down actor Tom Sizemore turned into a violent, abusive relationship.
Fleiss said yesterday that one of the most violent incidents occurred in May 2002 after Sizemore returned from filming a movie in Canada.
Tabloids were reporting that he was cheating on her with prostitutes, and a number of the women called Fleiss to say Sizemore owed them money, she testified at the start of his domestic abuse trial.
"I confronted Tom and we had a horrible fight," Fleiss said. "He hit me in the bedroom and dragged me across the room by my hair. He hit me with his hands ... I had a black eye."
Fleiss left the house with a friend of Sizemore. When she later returned for her property, she said, she found Sizemore had broken everything of hers.
Sizemore, 41, has pleaded not guilty to 16 misdemeanour counts of hitting, harassing and threatening Fleiss, his ex-fiancee.
The charges include counts for making about 100 harassing telephone calls to her in a one-year period, vandalism, threatening to inflict injury to a person or property, and corporal injury resulting in a traumatic condition.
Sizemore is free on $US100,000 ($A155,000) bail. He faces up to 13 years in prison if convicted of all the charges.
In court, Fleiss wore a gray suit jacket over a black miniskirt and stiletto heels. She began to weep almost as soon as she sat down. Sizemore sat across the courtroom and avoided eye contact with her as he took notes.
Fleiss previously served 21 months in prison for tax evasion and attempted pandering. She said she met Sizemore shortly after being released in 1999, fell in love with him and moved into his home in fashionable Benedict Canyon.
"I loved him a lot and I trusted him," she said at the outset of her testimony.
She said she was not dependent on him for money and knew he had a volatile temper. She said Sizemore's manager wrote up an agreement in which the actor promised not to abuse her or to break any of her property.
Things began to deteriorate in November 2001 when they went to New York to promote Blackhawk Down.
"We did the Howard Stern show and he asked me if I liked the movie," Fleiss testified. "I said I didn't like it because it was too violent. I like things like Bambi."'
When they went back to their hotel room, Fleiss said, Sizemore hit her so badly they couldn't go to the premiere of the film.
Asked why she didn't call the police, she said, "I was on parole, the convicted criminal, and he was the all-American guy and could send me back to prison."
Assistant City Attorney Robert Cha told jurors they would hear numerous tape recordings of Sizemore's threatening phone calls and "you will hear that this case is about jealousy, control and manipulation. The victim is Heidi Fleiss."
Sizemore's attorney, Michael Fitzgerald, said Fleiss was lying.
"You will hear evidence that contradicts Heidi Fleiss," he said. "There are no crimes here, just tragedies."
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text:
Elmo visited by Sugar Snow Baby Birthday Cake
when i made baby elmo, i was very excited coz he turned out very cute. One thing made me more excited is my very first sugar version of Snow Baby figurines. I love them so much but because they are not much affordable in my financial ability, i tried my best to make them in sugar. They are fun to do.
Tags: birthday red cake chocolate
2 Comments
Beautiful cake!
thank you FirstClassCakes


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InMinistry MAPMin Class
GSEM534 - Issues in Ellen G White Studies
Professor: Denis Fortin, PhD
Date: November 10-14, 2013
Location: Northern New England Conference Office (Westbrook, Maine)
Course Description:
Issues in Ellen G. White Studies looks into contemporary issues related to the ministry and writings of Ellen White. Among the issues discussed in this course are: Ellen White's role and ministry, her understanding of her inspiration and her authority, her methods of writings and use of theological and historical sources, her use of literary assistants, rules of hermeneutics for her writings, her role in the development of Adventist doctrines and lifestyle, and her relevance for today's Seventh-day Adventist Church.
Class Resources:
Reservation Form
Location Information
Attendee List:
- Jeffery Abatayo
- Steven Brownell (CEU)
- Greg Carlson (CEU)
- Jonathan Cross
- Paulette Heinlein
- Amanda Johnston
- Easton Marks
- Arnet Mathers (CEU)
- Brian Milano (CEU)
- Dorrin Patillo
- Cornel Preda
- Kevin Robert
- Edrol Sandy (CEU)


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Welcome To
Orlando Luxury Golf Villa Web Site
(Highlands Reserve, Orlando)
For Detailed House Specification (Broadband Required) Click Here
Youtube Videos Of The Villa: Please Click Here
This site was last updated 1st April 2015
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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Proteolytic enzymes are important macromolecules in the regulation of biochemical processes in living organisms. Additionally, these versatile biomolecules have numerous applications in the industrial segment. In this study we have characterized a protein-rich fraction of
Proteases (EC 3.4), also known as proteolytic enzymes or peptidases, are a large group of enzymes which play a significant role in many biochemical mechanisms to maintain metabolic processes of all organisms [
Examples of serine proteases isolated from plants are ATFE (
In this study, we report for the first time the pharmacological properties of a protein-rich fraction of
Proteases are proteolytic enzymes naturally found in all organisms [
In order to assess the presence of proteolytic activity in protein extracts of
Azocaseinolytic activity of
F1.0 was resolved into several protein bands ranging from 150 kDa to 6.5 kDa when subjected to SDS-PAGE (
SDS-PAGE profile of fraction F1.0 proteins and in-gel protease assay (zymography). (
Among proteolytic enzymes, those which hydrolyze fibrinogen have been related to important effects on the coagulation cascade. Studies conducted with these molecules could contribute to the development of molecules of interest in the treatment of disorders associated with the coagulation cascade, beyond the possible application in the clinical laboratory [
Fibrinogenolytic activity of
The kinetics of fibrinogen degradation was determined by incubation of enzyme source plus substrate for 0.5, 1, 2, 3, 4, 5, 10, 15, 20, 30, 60 and 120 min. The incubation of F1.0 with bovine fibrinogen showed that the enzyme hydrolyzed Aα chain very efficiently within 1 min of incubation, Bβ within 4 min, while the hydrolysis γ chain is slower (
In order to explore the class of protease involved, an inhibition study was carried out using different class-specific inhibitors (E-64, PMSF and EDTA). The fibrinogenolytic activity of F1.0 was inhibited by E-64 (
Fibrinolysis is another important proteolytic event that is associated with wound healing. During physiological processes, plasmin is the key enzyme that hydrolyzes fibrin clots [
In order to verify the presence of fibrinolytic activity, plasma clots were incubated with F1.0 at different protein concentrations for 1 h at 37 °C. The reaction was stopped by addition of SDS-PAGE sample buffer in the presence of β-mercaptoethanol and immediately subjected to 100 °C for 5 min. The hydrolysis pattern was visualized by SDS-PAGE (
Dose-dependent hydrolysis of plasma clot by fraction F1.0 of
The procoagulant activity of F1.0 was determined by measuring its effect on the clotting time of human citrated plasma in a digital coagulometer. The clotting time was reduced after addition of increasing concentrations of F1.0 (
Effect of
We also investigated the effect of F1.0 on blood coagulation by determination of activated partial thromboplastin time (APTT) and prothrombin time (PT). APTT decreased from 41 s to 14 s when the concentration of protein increased (
Fibrinogenolytic enzymes act by cleaving the fibrinogen molecule at sites distinct from those cleaved by thrombin, turning the fibrinogen incoagulable [
Cysteine proteases present in the latex of
The effect of pH on the azocaseinolytic activity of F1.0 was monitored from pH 2.0–12.0. F1.0 was active at a broad pH range, especially at pH of 5.0 (
The effect of some metal ions and detergents on azocaseinolytic activity was analyzed (
Effects of pH and temperature. (
The nonionic surfactant Triton X-100 did not affect the enzymatic activity significantly (
Effects of metal ions and detergents on the azocaseinolytic activity of fraction F1.0. # Control (H2O) with 100.0 ± 1.7 relative activity. *
|Metal ion and detergents
|% Relative activity #
|1 mM
|81.8 ± 1.1 ***
|1 mM
|82.4 ± 1.3 ***
|1 mM
|12.1 ± 3.6 ***
|1 mM
|2.3 ± 3.0 ***
|1 mM
|1.8 ± 6.0 ***
|1 mM
|5.1 ± 2.3 ***
|1 mM
|88.6 ± 3.6 **
|1 mM
|56.1 ± 1.1 ***
|1 mM
|15.5 ± 3.9 ***
|Tween 20
|92.5 ± 1.1 *
|106.4 ± 2.3
|36.3 ± 3.9 ***
For enzymes belonging to the class of cysteine protease, the assessment of hydrolytic activation with thiol-specific reducing agents such as
Effect of reducing agents on the azocaseinolytic activity of fraction F1.0. (
Azocasein, bovine fibrinogen, albumin from bovine serum, and protease inhibitors E-64 and phenylmethylsulphonyl fluoride (PMSF) were purchased from Sigma Aldrich (St Louis, MO, USA). Bicinchoninic acid (BCA) assay kit was purchased from Pierce (Rockford, IL, USA). All reagents of SDS-PAGE were purchased from GE Healthcare (Piscataway, NJ, USA). All other reagents and solvents used were of analytical grade. The water used was purified by reverse osmosis. The leaves used for characterization of biological activity in this work were collected in Ceará-Mirim city (5°36'08.34''S 35°26'48.77''O), Rio Grande do Norte, Brazil. The species studied was identified in the "Herbário Parque das Dunas", Department of Botany and Zoology, at the Federal University of Rio Grande do Norte, where the specimen was inserted into the collection with protocol number 14,065. The human blood samples were collected from healthy volunteers.
Male and female Swiss mice (32 g ± 3 g) 6–8 weeks of age were obtained from the "Biotério de Criação de Animais do Centro de Ciências da Saúde da UFRN". The animals were maintained under a controlled temperature (22 ± 2 °C) and with free access to water and commercial feed. The experiments were performed in accordance with the guidelines established for the care of laboratory animals. This study was approved by the "Research Ethics Committee on Animal Use" at the Federal University of Rio Grande do Norte, under protocol number 028/2009.
Samples of plant tissue previously washed with distilled water were pulverized in liquid nitrogen. The fine powder was weighed and added to buffer 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.5 in the ratio 1:2 (w/v), under stirring at 4 °C for 2 h. After centrifugation for 30 min at 12,000 g at 4 °C, the supernatant (crude extract) was precipitated with acetone (1:2, 1:1 and 2:1, v/v, acetone: extract) at 4 °C for 4 h, to obtainment of fractions F0.5, F1.0 and F2.0, respectively. After centrifugation for 30 min at 12,000 g at 4 °C, the precipitates were collected and dried under vacuum (Concentrator Plus, Eppendorf, Hamburg, Germany) and then resuspended in the same extraction buffer and stored at −20 °C.
Protein concentrations were analyzed using the BCA assay kit following the manufacture's instruction. Aliquots of sample (25 μL) were added to microwell plate and mixed with BCA reagent (200 μL). The plate was incubated for 30 min at 37 °C and the absorbance measured at 562 nm with a microwell plate reader (µQuant™–BioTek, Winooski, VT, USA). Bovine serum albumin (BSA–Sigma) (0.025–2.0 mg/mL) was used as a standard calibration curve.
The azocaseinolytic activity of F0.5, F1.0 and F2.0 was performed as previously described [
The electrophoretic profile of F1.0 was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) 15%, using the minigel system GE Healthcare (Piscataway, NJ, USA) [
Fibrinogen hydrolyzing activity of fraction F1.0 was determined as previously described [
In-gel protease assay was performed as previously described [
Clotting activity of F1.0 was studied using citrated human plasma, as previously described [
APTT and PT were measured in CLOTimer analyzer, as previously described [
The plasma clot hydrolytic activity of fraction F1.0 was studied using citrated human plasma, as previously described [
Defibrinogenant activity was tested using the method of Gene
The hemorrhagic activity test was performed as described in Nikai
Blood samples from healthy non-smoking volunteers were collected in K3EDTA (1.5 mg EDTA: 1 mL blood) test tubes, washed three times with saline and subjected to centrifugation at 800 g for 10 min between each washing, from which 20% red blood cell (RBC) suspension was prepared. Serial dilutions of fraction F1.0 (50–1000 µg) were added separately to each microtube containing 1 mL 0.2% RBC suspension and incubated at 37 °C for 1 h. The tubes were centrifuged for 10 min at 800 g and the degree of RBC lysis was assessed by measuring the optical density of the supernatant at 540 nm wavelength. The results were compared with negative non-treated control and a positive control (100% lysis) in which normal saline was substituted with distilled water.
The azocaseinolytic activity of F1.0 was monitored at varying pH values, to determine the optimum pH, using the specified buffers in the pH range of 2–12 as follows: for pH 2–5: 0.05 M sodium acetate buffer; pH 6–7: 0.05 M sodium phosphate buffer; pH 8–9: 0.05 M Tris-HCl; and for pH 10–12: 0.05 M glycine buffer. The optimum temperature was determined in the temperature range of 4 °C to 80 °C and the enzymatic activity of fraction F1.0 was assayed by the azocaseinolytic assay described above.
The effect of metal ions on the enzyme activity was determined using different salts in 0.05 M Tris-HCl, 0.15 M NaCl, buffer, pH 7.5. Fraction F1.0 pre-incubated with 1 mM of the salts CoCl2, NiSO4, MnCl2, CaCl2, MgCl2, CuSO4, HgCl2, BaCl2 or ZnCl2 (at 37 °C for 15 min) were tested and the assay was performed in triplicate using azocasein 1% as a substrate. Alternatively, fraction F1.0 was pre-incubated with 1 mM or 5 mM of the reducing agents DTT, β-mercaptoethanol or
One-way ANOVA with Tukey's post test was performed using GraphPad Prism version 5.00 for Windows, GraphPad Software (San Diego, CA, USA).
The study of a protein-rich fraction of
The authors thank CNPq and CAPES (Brazil) for the financial support and Elizeu Antunes dos Santos for the infrastructure made available at the Laboratory of Chemistry and Function of Bioactive Proteins (Department of Biochemistry/UFRN) and the students Ilanna Tainá Medeiros Gurgel Pinheiro and Argos Henrique Albuquerque Costa. MF Fernandes-Pedrosa gives thanks to CNPq for Scholarship in Research Productivity. The authors are also grateful to Andrew Alastair Cumming and Andréia Bergamo Estrela for editing this manuscript.
Conceived and designed the experiments: Yamara A. S. de Menezes, Matheus de F. Fernandes-Pedrosa, Ivanise M. M. Rebecchi, Adeliana S. de Oliveira and Adriana F. Uchoa. Performed the experiments: Yamara A. S. de Menezes and Juliana Félix-Silva. Analyzed the data: Yamara A. S. de Menezes, Juliana Félix-Silva, Ivanise M. M. Rebecchi, Adriana F. Uchoa and Matheus de F. Fernandes-Pedrosa. Contributed reagents/materials/analysis tools: Matheus de F. Fernandes-Pedrosa, Arnóbio A. da Silva-Júnior, Adriana F. Uchoa and Ivanise M. M. Rebecchi. Wrote the paper: Yamara A. S. de Menezes, Arnóbio A. da Silva-Júnior, Ivanise M. M. Rebecchi, Adriana F. Uchoa and Matheus de F. Fernandes-Pedrosa.
The authors declare no conflict of interest.


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A Star is Milked
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A Star is MilkedA Star is Milked (1988) Season 2 Episode 19A- Mighty Mouse: The New Adventures Cartoon Episode Guide
This page has been viewed 16 times this month, and 339 times total.
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- 1988
- Ralph Bakshi Animation Productions, Bakshi-Hyde Ventures, Viacom Productions Inc.
- Paramount Television, Viacom International
- Mighty Mouse: The New Adventures Episode Guide
- Cartoon Characters: Mighty Mouse/Mike Mouse, Pearl Pureheart, Orphan Scrappy, Bat-Bat (Bruce Vein), Sourpuss, The Cow, Fractured Narrator.
- Vocal Talent: Patrick Pinney (Mighty Mouse/Mike Mouse), Maggie Roswell (Pearl Pureheart), Dana Hill (Orphan Scrappy), Charles Adler (Bat-Bat/Bruce Vein), Joe Alaskey (Sourpuss), Michael Pataki (The Cow), Beau Weaver (Fractured Narrator), Rodger Bumpass, Candy Candido, Joe Colligan, Jeannie Elias, Bill Farmer, Ellen Gerstell, Tami Holbrook, Mona Marshall, Janet May, Terence (Terry) McGovern, Lisa Raggio, Clive Revill, Neil Ross, Pamela Rowan, Jim Ward, Wendell Washer, Alan Oppenheimer.
- Directed By Ralph Bakshi, Kent Butterworth, Eddie Fitzgerald, Steven E. (Steve) Gordon, Bob Jaques, John Kricfalusi, David Marshall, Tom Minton, Jim Reardon, John Sparey, Bruce Woodside.
- Produced By Ralph Bakshi.
Executive Producer: John W. Hyde.
- Written By Eddie Fitzgerald, Nate Kanfer, Tom Minton, Doug Moench, Rich Moore, Jim Reardon, Andrew Stanton.
Story Direction: Ralph Bakshi.
- Edited By Baylis Glascock.
- Animation Director (Overseas): Dave Marshall.
- Story Editor: Tom Minton.
- Director of Photography: John Kricfalusi.
- Casting: Sherry Argaman, Carole McCoy, Janelle Pransky.
- Executive in Charge of Production: Tom Klein.
- Production Managers: Jan Adams, Michael Lander.
- Layout Supervisor: Bruce W. Timm.
- Key Layout Artist: Brian Mitchell.
- Layout Artist: Eric Stefani.
- Storyboard Artists: Ralph Bakshi, Kent Butterworth, Umberto De la Fuente, Steven E. Gordon, Kevin Harkey, Tom Minton, Rich Moore, Brad Rader, Jim Reardon, Jim Smith, Byron Vaughns.
- Sound Editor: Kent Holaday.
- Sound Mixer: Sherry Klein.
- Sound Effects Editor: Jeff Vaughn.
- Sound Effects: Lewis (Lew) Goldstein.
- Music Editor: Mark von der Heide.
- Music Supervision: Lynne McCleerey, Mitch Wirth.
- Music Producer: Gary Anderson.
- Lyricist: Marshall Barer.
- Character Color Designer: Rose Ann Stire.
- Character Color Designer: Kevin Linda Redondo.
- Background Designer: Vicky Jenson.
- Character Model: Jeff Pidgeon.
- Color Key: Vicky Jenson.
- Transportation Coordinators: Mike Kazaleh, Lynne Naylor.
- Voice Tracks Recorded At: Buzzy's Recording Services.
- Audio Post-Production: EFX Systems.
- Music By: Score Productions Inc.
- Video Post-Production: Unitel Video.
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SVC Chaos: SNK vs. Capcom
Review by Numan_lord
"Shin Akuma strikes again!"
I was a very big fan of Capcom vs. SNK 2 so when I seen SVC: Chaos I didn't hesitate to buy it at all, and for a price of $20.00 it was all worth it.
Graphics: 8 / 10
The graphics in this game have the right to receive some criticism this time on SNK's part due to the fact that the majority of their characters have been copied and pasted from older SNK fighting games I'm talking KOF '95-97 looking sprites we have here. The backgrounds in this game are quite dull too, not exciting at all. The real shine in the graphics department is definitely the new smooth looking Capcom characters like Akuma, Ryu and Demitri and new SNK characters like Mr. Karate, Athena and Mars People. The newer characters in this game give the graphics the fairly high score.
Sound: 7 / 10
The sound in this game is also relatively decent. All the characters talk (mostly Japanese :P ) when they fight and even in their intros (Not the on screen readable intros). The music is pretty good too it all tends to suit the not so great scenery your characters battle within. The music at times can be quite dull itself but only in some instances like the final stage where you fighter either Serious Mr. Karate or Shin Akuma. I was expecting more epic music for two epic bosses. The secret bosses Red Arremer and Athena have decent music for their stages. Athena's heavenly music suits her stage well and Red Arremer's spooky music suits his fine too.
Storyline: 7 / 10
The storyline isn't all too great it is just a dream match game with different universal characters battling it out to see who is stronger. But it seems to work in the end.
Characters: 8 / 10
The character roster in this game is pretty much A standard material. We have on the SNK side a mixture of KOF, Samurai Shodown, Metal Slug, Athena, and Art of Fighting. On the Capcom side we have Street Fighter, II & III, Darkstalkers, Red Earth and Ghosts N' Goblins. Me seeing Red Arremer, Athena, Hugo and Demitri in this game just made everything all the more awesome considering they are all very unique and awesome characters. But knowing that my favourite fighting machine Shin Akuma was present baked the cake fore me. It would have been nice to see Gill in this game too but that never happened obviously.
Gameplay: 8 / 10
The game play is just like any other fighting game. But many Capcom fighting game fans would know that performing super moves requires a lot of skill and timing. With SNK fighting games they tend to just let loose all the big explosions an aura blasts like their's nothing to it. This can be a good thing and a bad thing. The good side you can do most moves with ease and totally own your opponent. The bad thing you can let loose moves you didn't mean too thus you will loose power bar levels stooping you from performing other stronger moves. You then have to fill your bar back up full and you may kill the opponent by accident while doing so if your accident move connected with them. The super arts in this game or Exceed moves as they are known are very powerful and can wipe an entire health bar clean from any character almost. This is why this game gives you two health bars per fight and to help out the balance so fights don't last a whole 2 seconds like in the very sad Samurai Shodown V. You can only use an exceed move if your character has lost one health bar and has a full 3 stock power bar. Also after using an exceed move whether it connects with the opponent or not you cannot use another exceed until your next opponent. The gameplay in this game is also much harder than most fighting games. So changing the difficulty settings won't give you a great difference at all. And trust me survival mode is just so very cheap you'll be flat out getting past the second fight. Myself having played as Shin Akuma in arcade mode and doing well; decided to take him into survival. But using him in this survival mode was a real eye opener to me considering I still haven't unlocked Red Arremer or Athena I have only just fought them and lost miserably to them both. :P
Replay Value: 8 / 10
Much like any fighting game you will always find yourself coming back for another decent dose. Even if this game is quite hard the unique character roster and onscreen readable feud intros make it all worthwhile.
Overall: 8 / 10
Basically this is kind of like SNK's version of Capcom vs. SNK 2 and although not as impressive as that game, it has a lot of unique characters and gameplay elements only found in this fighting game. It's definitely worth a play.
Reviewer's Rating: 4.0 - Great
Originally Posted: 02/27/09, Updated 08/05/10
Game Release: SVC Chaos: SNK vs. Capcom (EU, 04/15/05)
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