text:
Photo Call Takes Lead, Victory in Orchid
Teresa Viola Racing Stables' grade I winner Photo Call returned to her winning ways in the $200,000 Orchid Stakes (gr. IIIT) April 2 at Gulfstream Park.
Teresa Viola Racing Stables' grade I winner Photo Call returned to her winning ways in the $200,000 Orchid Stakes (gr. IIIT) April 2 at Gulfstream Park.
Photo Call, good enough to run fifth in the Breeders' Cup Filly & Mare Turf last year, will have to contend with stakes winner Quiet Kitten in a relatively soft field for Orchid Stakes
While she will start from the outside post in a field of 12, Goldy Espony will try for a fifth win in six starts when she goes in the $100,000 La Prevoyante Handicap (gr. III) Saturday.
Ken and Sarah Ramsey's multiple grade I winner Stephanie's Kitten returns in the June 5 New York Stakes (gr. IIT) at Belmont Park. The daughter of Kitten's Joy won Tampa Bay's Hillsborough Stakes (gr. III) last time out.
Grade I winner Discreet Marq will make her first start of 2015 at Belmont Park May 9, entered as the 2-1 favorite in a field of seven older fillies and mares for the $150,000 Beaugay Stakes (gr. IIIT).
Bethlehem Stables, Michael Dubb, and Highclere America's Rosalind snapped a six-race losing streak when she held off the late run of Maximova to take the $200,000 Sheepshead Bay Stakes (gr. IIT) at Belmont Park May 2.
Kept in training, Robert Evans' Irish Mission returned immediate dividends, taking the $150,000 Very One Stakes (gr. IIIT) at Gulfstream Park Feb. 21.
Christophe Clement is enjoying the best year of his career since going out on his own in 1991 with almost $9 million in purse earnings, including a first victory in the Belmont Stakes (gr. I) with Tonalist.
With the benefit of a perfect ground-saving trip under John Velazquez, Aigue Marine rallied up the rail to catch favored Danza Cavallo in the $200,000 Long Island Handicap (gr. IIIT) on yielding turf Nov. 8 at Aqueduct.
White Rose emerged from a five-horse scramble in the final 50 yards to win the $125,000 Rood & Riddle Dowager by a nose over lukewarm favorite Caroline Thomas Oct. 19 at Keeneland.
Coming off a victory in the Noble Damsel Stakes (gr. IIIT) in her U.S. debut, George Bolton and Peter Leidel's Annecdote tops a solid field of 12 older fillies and mares entered in the Athenia Stakes (gr. IIIT).
It wasn't the Belmont Stakes (gr. I) , but it was another stakes winner for owner Robert S. Evans and trainer Christophe Clement when Irish Mission ran down Viva Rafaela to take the $150,000 Glens Falls Stakes (gr. IIIT).
Juddmonte Farms' Riposte may be the class of the field in the $200,000 Sheepshead Bay Stakes (gr. IIT) May 24, run over 1 3/8 miles on Belmont Park's inner turf course.
Inimitable Romanee, given a patient ride by Alan Garcia, got up at the wire on the far outside to nip Aigue Marine for a 14-1 surprise in the $150,000 Bewitch Stakes (gr. IIIT) April 24 on the Keeneland turf.
Princess of Sylmar used the $100,000 Cat Cay Stakes as a good starting point for the 2014 season at Aqueduct Racetrack April 6, gliding through an opening off the turn and cruising to victory.
Distance-loving Inimitable Romanee aims for her third graded stakes win in a row when she takes on eight other older filly and mare rivals in the $150,000 Orchid Stakes (gr. IIIT) on the grass March 29 at Gulfstream Park.
Stonestreet homebred Teen Pauline put on another front-running show at Aqueduct while rolling to her third straight win and first graded stakes victory in the $200,000 Top Flight Handicap (gr. II) March 1.
Stonestreet's Teen Pauline seeks her first graded stakes score and third straight stakes win March 1 at Aqueduct, when she takes on a small but salty field of fillies and mares in the $200,000 Top Flight Handicap (gr. II).
Although she hasn't run since Oct. 5 and hasn't won since June 20, Juddmonte Farms' Riposte looks to have a decisive class edge over her seven opponents in the $150,000 Suwannee River Stakes (gr. IIIT) Feb. 8 at Gulfstream.
Ken and Sarah Ramsey's Kitten's Dumplings proved a quirky winner of the $400,000 Queen Elizabeth II Challenge Cup over 8-5 favorite Alterite at Keeneland Oct. 12 to pick up her first grade I victory.
TimeformUS Weekend Plays: The Queen Elizabeth II Challenge Cup and the Athenia read blog
Tom LaMarra and Claire Novak handicap the Queen Elizabeth II Challenge Cup Stakes at Keeneland. Will one of the Kitten's win this weekend? Download Brisnet Past Performances for the Queen Elizabeth II Challenge Cup Stakes.
Alterite, a 1 1/2-length winner of the Garden City Stakes (gr. IT) in her North American debut last month, heads a field of nine 3-year-old fillies entered for the $400,000 Queen Elizabeth II Challenge Cup (gr. IT).
The Sept. 14 Garden City Stakes (gr. IT) at Belmont Park offers bettors an interesting dilemma, with talented grade I winners facing a grade I-placed French import and the one-two finishers of the grade II Lake Placid Stakes.
Caroline Thomas picked up the first stakes win of her career when moved up in the $200,000 Woodford Reserve Lake Placid Stakes (gr. IIT) due to the disqualification of original winner Nellie Cashman Aug. 18 at Saratoga.
|Breeder / Owner
|Alternative Meds
|David P. Duggan
|Caroline Thomas
|Barclay Tagg
|Flash Forward
|Dominick Schettino
|Golden Joop
|Kiaran P. McLaughlin
|Magnificent Shirl
|Jerry Hollendorfer
|Mariel N Kathy
|Dominick Schettino
|Christophe Clement
|With Sugar On Top
|Kiaran P. McLaughlin
|Real Good Deal S.
|Prairie Gold Juvenile S.
|Curlin S.
|Duke City Sprint S.
|Arapahoe Debutante S.


text: Iv been doing squats for a long time but recently I have decided to go deeper and heavier. My knees have been hurting for the past couple weeks. Is this something that will go away once i get used to going deeper? Or is it most likely a fault with my technique?

subset: commoncrawl


text:
After rolling up 1,715 yards from scrimmage as a rookie in 2008 (1,238 on the ground), Bears RB Matt Forte is struggling to find room to run in 2009.
After three games, he's rushed for 150 with only 2.5 yards per tote.
So what's wrong? Is an offseason hamstring injury lingering?
"No, my hamstring does not hurt at all," Forte says.
Is an overhauled offensive line sporting three new starters to blame?
"When we put our mind to it, we can run against any team," says Forte, though he did not lay the blame on his linemen's shoulder pads. "It's difficult running in the NFL. We know that. But if we get bodies on people and stay strong, we can run the ball."
This Sunday, the Bears host the suddenly scalding Lions, whose run defense is ranked a respectable 16th.
"You just have to keep building, keep trying to get better," said LT Orlando Pace, who was signed April 2, the same day QB Jay Cutler arrived in the Windy City. "Every week, we're just going to try to get this thing going. We just have to work harder this week."
Along with Pace, second-year RT Chris Williams is a new starter as is LG Frank Omiyale, another free-agent addition. -- Nate Davis
Hat tip: Chicago Tribune
Nate Davis is a reporter, blogger and editor who's been at USA TODAY since 2000. He has covered the NFL since 2005. No, he did not play quarterback for Ball State. Davis' succession of our esteemed colleague Sean Leahy at The Huddle is considered a Brady-for-Bledsoe swap by most "insiders."More about Nate


text:
GATESVILLE — Annabelle Barrington, a Gatesville High School senior, will represent the Gatesville Boys & Girls Club at the State Youth of the Year competition in Austin in April.
"Annabelle is an amazing young woman," said Gatesville club Director Julie Hudson, who nominated Barrington for the competition.
"She is at the club every day after school and was here all summer," Hudson said. "The kids love her."
Barrington won local and area Youth of the Year competitions to advance to the state finals, Hudson said.
"Boys & Girls Clubs of America's Youth of the Year recognition is the highest honor a Club member can achieve," according to a news release from Brian Jones, director of operations for the organization.
Founded in 1947, the competition recognizes young people "for service to their club and community, academic performance and contributions to their family," the release stated.
The daughter of Jim and Sherry Barrington, Annabelle is vice president of the Keystone Club at the Gatesville Boys & Girls Club, active in 4-H and is a member of the Gatesville High School Dazzlers dance team, Hudson said.
Barrington wrote two essays to qualify for the competition, one about what the Boys & Girls Club means to her, the other about her future plans, which include attending Central Texas College to pursue a career in Special Needs Education.


text:
Following the cancellation of this years event, Oxford Folk Festival is now looking to the future. We are patiently rebuilding, and you can expect a series of concerts and fund-raising events in the months to come. There is unlikely to be a full-scale festival in 2012 but we hope to be in full swing by 2013. Keep watching this space for further events.
In the meantime, visit our what's on page for details of special Christmas events.
Our great friends at Folk Weekend Oxford are planning another springtime jamboree to keep the festival spirit alive over the weekend of 20-22nd April 2012. Visit www.folkweekendoxford.co.uk
Also, check out Folk in Oxford, a brand new website which aims to showcase the wide range of folk and roots activities in and around Oxfordshire: http://folkinoxford.co.uk


text:
Since 2000, Efficiency Vermont, the statewide efficiency utility has worked to help Vermonters lower their electric bills through technical assistance and rebates.
Every year, the entity, which is funded through an energy efficiency charge that is added onto customers' electric bills, produces a report highlighting the savings it produces for the state.
While many cheer Efficiency Vermont's efforts to save people money, some businesses that bear a heavy burden of the cost are not convinced that a mandatory charge tacked onto everyone's bill is the best way to go about funding efficiency.
VTDigger.org set out to determine just how much larger businesses pay for the efficiency charge and if it is worth it for the state's big energy users.
The Vermont Energy Investment Corp., a nonprofit, operates Efficiency Vermont under an appointment issued by the Vermont Public Service Board. Formerly, the organization operated on a contract basis. In 2010, the board granted the organization an order of appointment to serve as a regulated efficiency utility — something business groups opposed.
So whether they like it or not, everyone pays the charge, and Efficiency Vermont will be offering its services through 2021 with its current appointment.
As for big energy users, Efficiency Vermont would not divulge usage data for the largest users. It claims it is not subject to the state's public records law and that the information is confidential anyway.
Information provided by the efficiency utility shows that its 50 largest customers used 864,805 megawatt hours of electricity in 2010 — not including IBM, which operates its own efficiency program, and Omya, which was owned by Vermont Marble Power at the time (a utility itself that did not have to pay the charge). That is about 15 percent of the approximately 5.6 million megawatt hours used in 2010 statewide, according to the Department of Public Service. For 2011, the budgets for all energy efficiency utility programs totaled around $40 million. Efficiency Vermont estimates that those top 50 users contributed about $5.9 million through the energy efficiency charge. The organization claims members in that group that took advantage of Efficiency Vermont programs reaped $15.8 million in total resource benefits, which is the lifetime value of the savings that will result from energy efficiency measures installed by these customers in 2010.
Who actually pays what into the energy efficiency charge is a bit complex since residential, commercial and industrial payers each pay different rates. They each amount to a fraction of a cent per kilowatt hour, with industrial users paying the smallest amount per kilowatt hour. Some customers pay "demand" rates which makes for even less of an apples-to-apples comparison, and Burlington Electric Department customers pay a different rate that funds that utility's own program.
The bottom line is that the increasing budgets for efficiency programs increases charge rates across the board generally. For 2012, 2013 and 2014, the budgets for efficiency programs will be $40.1 million, $42.8 million and $45.9 million respectively. That means an increase in the energy efficiency charge of 4.2 percent, 6.7 percent and 7.2 percent for each of those years.
In 2012, a change in the ownership of Omya's Vermont Marble Power Division (a utility) created another wrinkle in the efficiency charge story. Previously, Omya had not been paying the charge on its power much like other utilities like Green Mountain Power do not pay the charge for the energy they use. In 2011, CVPS purchased Vermont Marble Power. This means the electricity used by Omya is now subject to the charge, offsetting what other ratepayers pay and decreasing the charge amount for industrial customers. Representatives from Omya were not available to comment on this issue.
Talking to businesses and industry representatives, the consensus seems to be that if ratepayers are going to pitch in millions of dollars every year for Efficiency Vermont, the way it operates needs to be, well, efficient.
Efficiency Vermont boasts $115.3 million in these total benefits to the state for the last year in its 2010 savings claim. Whether the operation is spending ratepayer dollars in the best manner is still up in the air, according to industry groups.
In comments to the Vermont Department of Public Service regarding the draft Comprehensive Energy Plan, the Associated Industries of Vermont took umbrage with the increase in Efficiency Vermont's budgets approved by the Public Service Board.
William Driscoll is the vice president of Associated Industries of Vermont. He points out that the organization, which lobbies on behalf of Vermont manufacturing and other business sectors, is by no means against efficiency. He recognizes that efficiency programs are good for the environment and can save businesses big money on their electric bills. AIV's beef is with the structure and method of going about promoting efficiency.
AIV criticized increasing the budget for the efficiency utility in its comments on the state's draft Comprehensive Energy Plan and submitted testimony in a proceeding before the Public Service Board regarding the structure of the energy efficiency utility.
Driscoll said the core of the problem is that the energy efficiency charge adds quite a bit to the cost of electricity, and for businesses, that can mean lost opportunities along with the increased cost of doing business.
While businesses take advantage of the efficiency rebates and expertise Efficiency Vermont offers, Driscoll said his organization hears from a lot of companies that what they gain in efficiency measures is not enough to cover the cost of the charge.
"Some companies have to spend so much on the efficiency charge that they lose other opportunities," Driscoll said.
He said tax incentives or a lending mechanism that would allow businesses to get funding for projects then pay it back themselves make more sense.
"At this point, it's a tax that everyone pays but only a few benefit," he said.
The issue with the utility structuring was whether to shift VEIC's role from a contractual status with the Public Service Board to an order of appointment like any other utility. The Public Service Board issued the order of appointment in December 2010. It runs through 2021.
AIV and IBM supported keeping the competitive bidding process.
According to testimony before the board, IBM had paid $35 million for 446 million kilowatt hours of electricity in 2008. It paid $1.5 million for the efficiency charge. The company would later develop its own pilot efficiency program.
Driscoll said the problem with an order of appointment rather than competitive bidding every three years is it can lock in any bad habits or poor practices going on at the utility.
Without meaningful opportunities or incentives to compete, Driscoll says, the energy efficiency utility, like any business, will be less innovative or cost effective.
For many individual businesses, the efficiency charge is a mixed bag. It increases the cost of doing business but it can really pay off in the form of rebates on big investments.
Bill Stenger, president and co-owner of Jay Peak Resort, has been working with Efficiency Vermont since the beginning. The resort has upgraded facilities and retrofitted just about everything it can to be more efficient, Stenger says. Stenger said the resort pays about $1 million a year for electricity, and with a new indoor water park opening this winter, costs will likely be even higher.
In the past, because of credits and rebates, the resort was able to cut down on the investment costs for a lot of new machinery like chairlifts and snow guns and invest in efficiency measures. With any new project, he says, the resort tries to involve the energy efficiency utility.
"The resources are useful and helpful," Stenger says.
But after doing about all it can on the efficiency front, the resort still pays a hefty price each year for the surcharge: $40,000 more or less.
"The bottom line question is: How much do we pay to fund those services, and is it equal to the benefit?" Stenger said.
He said he does not advocate eliminating the energy efficiency utility, but it needs to run a tight ship. There needs to be vigilant, aggressive oversight, if the program is going to work, Stenger said.
And there is oversight. The Public Service Board issued a request for proposal for an independent auditor to review and verify the savings Efficiency Vermont achieved and reported from 2008 to 2010. The audit is required every three years by statute. The last audit in 2008 found the program was in fact very effective and returned $1.61 for each dollar spent in the business sector.
Jim Merriam, director of Efficiency Vermont, says the majority of businesses appreciate the technical expertise the utility offers. It allows them to receive consultation on lighting one year and heating and cooling the next without having to hire experts in-house.
"Businesses clearly understand that to be able to get a benefit from Efficiency Vermont they need to participate and work with Efficiency Vermont," he said. "Once they do that, they see that we can really create a return on investment."
This December, Terry Kinter, director of operations at Fairbanks Scales in St. Johnsbury, offered a tour of the manufacturing facility's new state-of-the-art lighting system.
The business is one of 60 that took Efficiency Vermont's "Energy Leadership Challenge" to reduce their energy usage by 7.5 percent over two years.
Fairbanks Scales will be able to pay off the renovation in the company's 250,000-square-foot facility in five years, Kinter said. With help from Efficiency Vermont, the company was able to meet its bottom line and potentially save about $37,000 annually.
The Fairbanks Scales project helped another local business, Sutton Valley Electrical, obtain a large contract to install the new lighting system.
IBM, the largest private employer in the state and likely the largest energy user, has taken its own approach to efficiency. Currently, IBM is in the second-year of a three-year pilot program that requires the company to invest $1 million in efficiency measures. IBM is exempt from the charge because of this program, which started in 2009.
Janet Doyle, who works on energy and environmental programs at IBM in Vermont, said the idea of the self-managed program was to allow the company to retain capital it would have spent on the energy efficiency charge and put it to work on larger projects. Before, IBM would pay the energy efficiency charge, but it would be a year and a half to two years before it got the money back in the form of a rebate to invest in an efficiency project.
Unlike most companies, IBM had the technical expertise in house to go it alone. Highly-specialized technical staff allowed it to implement its own efficiency projects without the help of Efficiency Vermont.
Doyle said the company has reduced its electricity usage by 100 million kilowatt hours in the past 10 years.
"One of the results of our program is we've benchmarked it and found it's very cost-effective," Doyle said.
IBM recently added a new addition that replaces part of its cooling demand in the winter with cold air from outside. Even with a focus on efficiency, however, Doyle said it is difficult to keep up with rising energy costs.
Another large energy user, the University of Vermont, broke even last year with its efficiency measures that it pays for the Burlington Electric Department efficiency program.
According to Gioia Thompson, director of the university's Office of Sustainability, the institution spent about $160,000 on the energy efficiency charge and got back about the same in $100,000 in rebates and about $60,000 in expertise — about one full-time-equivalent salary for a year.
UVM has been working on efficiency since the 1990s with the Burlington Electric Department, which also now has an order of appointment from the Public Service Board.
Thompson said the benefits of rebates for new technologies that would not otherwise be on the market along with the technical expertise offered by the city electric department have been a big benefit for the university.
While businesses individually bear a substantial burden for their energy usage, according to numbers from Efficiency Vermont, multi-family housing constituted another large electricity usage sector with 420,000 megawatt hours used in 2009. Universities and colleges used 140,000. Ski areas 125,000, followed by K-12 schools, hospitals and water and wastewater facilities. So while big business takes big hits on the charge, individually ratepayers pitch in a massive amount also just in smaller doses.
Richard Sedano, director of United States programs for the Regulatory Assistance Project, said Vermont's focus on efficiency is a form of building business infrastructure. Having experts in the state to offer assistance as well as doing things like training contractors in the area of efficiency puts the state ahead of most others. Focusing on creating enough economies of scale to really tackle big projects and big programs is key, Sedano said.
One of the things that people forget, he said, is that not everything is directly cost-based. For example, while a business may not receive in benefits exactly what it pays for the energy efficiency charge, people in cities do the same thing. It costs more to serve people in rural areas than urban spots, but people in cities are OK with paying higher rates than it actually costs the utility to deliver power while rural residents actually pay less than the real cost for delivering electricity to them.
Looking at the big picture, the efficiency surcharge debate over who pays for it and how may be largely ideological. The bottom line, however, is that it is big money, and businesses (and ratepayers in the aggregate) shell out big dollars. The savings, according to Efficiency Vermont, are big too. The upcoming audit will have to verify just how big.


text:
Mouseover to zoom
Clawful (Classics)SKU: 027084918335 Price: $54.99 Write a review | No reviews for this product.
Clawful Bio
Real Name: Pronounced through a series of claw clicks
A member of the Karikoni, an Eternian race of crustacean warriors, "Clawful" as he was known outside of Orkas Island, became a core member of Skeletor's Evil Warriors after he saved Beast Man's life from a Seclapoid attack. Clawful's hard shell protects him from both extreme heat and cold. Under a spell cast by Evil Lyn, Clawful's dull wits were enhanced, turning him into a keen warrior and strategist. He fought alongside Skeletor, leading a battalion of Skeleton Warriors during the Second Ultimate Battle Ground. Clawful uses his dreadful claw to work his evil will!
Write a review | No reviews for this product.


text:
About Us
Gift Certificates
Gift Ideas
Sales & Promotions
Offline Order Form
Songs By Subject
Action & Participation
Art Appreciation
Character Education
Classroom Management
English: ESL/EFL/ESOL
Folk Songs
Holidays & Seasons
Music Appreciation
Nursery Rhymes
Reading & Writing
Social Studies
Special Education
All Subjects
Songs By Artist
Songbooks & Sheet Music
SFT Blog
Purchase orders accepted
Songs for a August Theme Unit
Song suggestions for the month of August.
The songs on this page are compiled from a variety of different artists and albums. We have grouped them together to help you narrow your search.
These August songs teach about summer weather, camping, the beach, and back to school.
En el bosque- Language Leaders
Mosquito- Jeanne Nelson and Hector Marín
Never Be Afraid to Ask Questions– Ben Stiefel
People Like It When I Say "Hi"– Cathy Bollinger
Picnic – Exploring Language through Song and Play
School is Cool– Lauren Mayer
School Song– Kathleen Wiley
She Likes Ice Cream – Tim Christenbury
Start this Day with a Song– Jack Hartmann
Track Detective– Jeff Wolin
August also has these Month Long Observances:
American Artist Appreciation Month
See our Art songs
National Inventors Month
See our Biographical songs
National Water Quality Month
See our songs about Water Conservation and Clean Water
First Week of August - National Clown Week
See our Silly Songs
Second Week of August
- National Apple Week
See our Fruit songs
- National Smile Week
See our Positive Attitudes songs
Third Week of August - American Dance Week
See our Dance & Movement songs
Holidays for the Month of August
August 1st
- Francis Scott Key's Birthday
See our vocal song, The Star Spangled-Banner
- Respect for Parents Day
See our Family and Character Education songs
- Sports Day
See our songs about Sports
August 3rd - National Watermelon Day
See our Fruit songs
August 5th
- American Family Day
See our Family songs
- Friendship Day
See our Friendship songs
August 6th - Wiggle Your Toes Day
See our Preschool Action songs
August 8th - Dollar Day
See our songs about Money
August 9th - Book Lovers Day
See our songs inspired by Children's Literature
August 10th - Herbert Hoover's Birthday
See our songs about US Presidents
August 19th -
- Orville Wright's Birthday
See our Biographical songs
- Bill Clinton's Birthday
See our songs about US Presidents
August 26th - National Dog Day
See our Pet songs
August 27th - Banana Lovers Day
See our Fruit songs
All Year- Intelli-tunes
En la playa (The Beach)- Language Leaders
French - À la plage (Beach)- Language Leadrs
Learning the Four Seasons
Months- Listen & Learn
Rhythms & Rhymes for Special Times- Jack Hartmann
Vamos al campo (Camping)- Language Leaders
See more songs for Theme Units.
Powered by Robly™
Songs for Teaching®
Using Music to Promote Learning
5501 W. Ross Dr.
Chandler, AZ 85226
800-649-5514 (480-689-1190)
© 2002-2012 Songs for Teaching® All rights reserved.


text:
Governor's Hurricane Conference Underway
(Orlando, Fla. - WFLA) The 28th Annual Governor's Hurricane Conference is going on all week in Orlando. This year's theme, "Rethink Risk" is intended to get attendees to re-focus on the very real threat tropical cyclones pose to Floridians, businesses and the many visitors to the Sunshine state.
Emergency managers and personnel, first responders, state, federal, volunteer agency, business and industry representatives along with health and medical professionals from around the state, nation and world will take part in 46 training sessions, 48 workshops and panel discussions to train and learn from each other.


text:
Illusions in advanced cancer : the effect of belief systems and attitudes on quality of life
Beadle, Geoffrey F., Yates, Patsy, Najman, Jackob M., Clavarino, Alexandra, Thomson, David, Williams, Gail, Kenny, Liz, Roberts, Syd, Mason, Bernard R., & Schlecht, David (2004) Illusions in advanced cancer : the effect of belief systems and attitudes on quality of life. Psycho-Oncology, 13(1), pp. 26-36.
|Accepted Version (PDF 229kB)
Patients with advanced cancer frequently express positive attitudes and can be unduly optimistic about the potential benefits of treatment. In order to evaluate an illusory domain in the context of advanced cancer, we developed a scale of will to live and characterized the beliefs that patients held about the curability of their cancer, and how committed they were to using alternative treatments. A measure of quality of life was used as the dependent variable in order to assess the association between these attributes. After a preliminary exploration confirmed the presence of an illusory domain, these concepts were prospectively tested in 149 ambulant patients with advanced cancer who attended for palliative systemic treatment, radiation treatment or supportive care. The scale of global quality of life was reliable (Cronbach's alpha coefficient 0.72). The distribution of the scores of will to live was skewed, with no respondent scoring poorly, and the scale was reliable (Cronbach's alpha coefficient 0.82). The scale of belief in curability showed diverse beliefs. In some cases, there was a discrepancy between respondents' beliefs in curability and what they believed to be the report by their doctors. There was also an association between a committed use of alternative treatments and a belief in the curability of the cancer (p<0.001). In a multiple regression analysis, both will to live and performance status remained associated with better quality of life scores after adjustment for other relevant variables (p<0.05 and <0.001, respectively). These results suggest that positive illusory beliefs can be measured and are an important component of adaption for some patients with advanced cancer. Furthermore, this illusory domain may influence the perception and measurement of quality of life.
Impact and interest:
Citation counts are sourced monthly from Scopus and Web of Science® citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.
Full-text downloads:
Full-text downloads displays the total number of times this work's files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
|ID Code:
|Item Type:
|Journal Article
|Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > NURSING (111000) > Clinical Nursing - Secondary (Acute Care) (111003)
|Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:
|Copyright 2004 John Wiley & Sons
|Copyright Statement:
|The definite version is available on publication at www3.interscience.wiley.com
|Deposited On:
|13 Oct 2005 00:00
|Last Modified:
|29 Feb 2012 13:10
Export: EndNote | Dublin Core | BibTeX
Repository Staff Only: item control page


text:
Richard E. Beamer, 83, of 300 S. 15th Street, Sebring, died on February 28, 2003, at 5:15 p.m. at th
Current subscribers have full access to all online news content as part of their subscription. If you are a current subscriber, please log in with your website account information for full access. If you are a current subscriber, but have not yet registered for access to the website, please call our Circulation Department at (330) 821-1200


text:
We're sorry, this listing is off the market. Try these listings instead:
Clean Well Established Dental Lab #405077 posted Apr 15, 2016
- Ohio, United States
- $360,000
The firm is a well established dental lab with many customers in the Central Ohio area as well as customers in Southern Ohio. The current facility was built in 2005 and is located in a medical office park that focuses on same day surgery and multiple dental specialties including oral surgery. The current...
Premium Dental Lab For Sale #465217 posted Mar 23, 2017
- Rockingham County, New Hampshire, United States
- $333,000
This is a very successful business with decades of experience servicing Southern New Hampshire. Their experience is in crafting crowns, bridges working with a variety of materials. This business is utilized widely through Southern New Hampshire. Services also include Veneers, full mouth re construction,...
Well-Established Dental Lab #471504 posted Jul 4, 2017
- Colorado, United States
- $225,000
Working with a variety of dental offices in the area, this lab has been in existence since 1974 and has become a valuable member of the dental care teams. Full dentures, removable partial dentures or fixed bridges, crowns, veneers, orthodontic appliances and splints are some of the work that this lab...
Did you know that social networking is a great way to buy a business? Sign up now and we'll show you how.
Disclaimer: DealStream has not independently verified any of the information in this listing and makes no warranty as to its accuracy or completeness.


text:
Cisco Ultra Packet Core, the Transition BeginsSP360: Service Provider
So yesterday we had our first snowfall up here in New England, holidays are coming fast, and so are events like the Consumer Electronics Show and Mobile World Congress. WellRead More
How Does XL Axiata Spell LTE Expansion – NFVSP360: Service Provider
Rapid advances in digital technology and the demand for high quality customer experiences, has spurred XL Axiata to develop a network solution capable of supporting their business strategy effectively andRead More
Hey Mobile Operators: "It's Time to Network Like the Big Guys, Innovate Like a Startup"SP360: Service Provider
Here we go, Mobile World Congress is fast approaching with about 100,000 attendees descending on Barcelona next week. The big focus this year is said to be around 5G, Internet-of-ThingsRead More
Telecom Asia Readers Rate Cisco VPC as NFV Innovation of the YearSP360: Service Provider
Telecom Asia has recently announced the winners of their 8th Readers' Choice & Innovation Awards. For the Cisco Mobility team it was a proud moment to receive the award forRead More
ACG Research says "Cisco Packet Core is Accelerating"SP360: Service Provider
Growing Market Share Leadership in Mobility – #1 in Packet Core, EPC, MME, SGW, & PGW for Q2 2015, driven by demand for LTE, Wi-Fi, VoWi-Fi, VoLTE, Policy Suite. Wasn'tRead More
Cisco Continues Mobile Core Momentum: Leads in Packet Core Market Share according to IHS Infonetics Research Data.SP360: Service Provider
Well I told you that we would be back real soon with another update and here you go. As you remember we recently blogged that ACG Research again confirmed that CiscoRead More


text:
Lifetime to Premiere New Unscripted Series CELEBRITY HOME RAIDERS, 3/6
Celebrities welcome viewers into their homes for a trip down memory lane and an exclusive look into their lives and prized possessions in Lifetime's all-new unscripted series Celebrity Home Raiders. The series premieres Thursday, March 6, 2014, at 10pm ET/PT.
In each half-hour episode of Celebrity Home Raiders, host Kit Hoover (Access Hollywood Live) and renowned auctioneers Darren Julien and Martin Nolan from Julien's Auctions, reminisce with celebrities as they search through their belongings for beloved treasures to be auctioned off, with proceeds benefiting a charity of each celebrity's choice. The stars invite Kit, Darren and Martin into their homes to discuss the HISTORY and sentiment of their personal memorabilia while haggling over each item's value. Potential items up for auction include David Hasselhoff's replica of KITT from Knight Rider, an Axe guitar owned and used by Gene Simmons from KISS, and many other highly coveted items. Will the objects' final price meet the celebrities' expectation and - more importantly - will they reach their charitable donation goals? The featured celebrities and their charities are:
· David Cassidy benefiting Alzheimer's Association of America
· David Hasselhoff benefiting Starlight Foundation
· Debbie Gibson benefiting Children International
· Fran Drescher benefiting Cancer Schmancer
· Ice T & Coco benefiting The Long Island Bulldog Rescue Group
· Lance Bass benefiting The Trevor Project
· Rick Harrison benefiting Voice Health Institute
· Simmons Family benefiting Sophie's Place Center for Abused Children
Celebrity Home Raiders is produced for Lifetime by DiGaVision (Teen Wolf) and Leftfield Pictures (Pawn Stars). Executive Producers are Liz Gateley and Tony DiSanto for DiGa Vision, Brent Montgomery, David George and Matthew J. Braley for Leftfield Pictures, Mary Donahue and Paul Hardy for Lifetime, and Lance Bass, who also is a creator of the project. Augi Jakovac serves as co-executive producer. Eight, half hour-long episodes have been ordered.
DiGa Vision, LLC is owned and controlled by former MTV producers and executives Tony DiSanto and Liz Gateley, and is focused on creating, developing and producing cable and broadcast reality and scripted series, films, and digital content. DiGa's signature style and unique voice reflects the pop culture relevance, distinctive visuals and seamless execution that DiSanto and Gateley are well recognized for. Since its launch in January 2011, DiGa has series and pilots in various stages of production at a slew of networks, including A&E, CMT, CW, History, Lifetime, Logo, MTV and Oxygen. Current series include Teen Wolf (MTV), Ke$ha: My Crazy Beautiful Life (MTV), Girl Get Your Mind Right (MTV), My Life is a Lifetime Movie (Lifetime), I Love The 1880s (History), The Baby Wait (Logo), and the upcoming adventure reality series Strange Medicine for Fusion, and the scripted horror series Scream for MTV. Digital series include K-Town with co exec producer Tyrese Gibson, ArtstTlk hosted by Pharrell Williams, travel series Ex-Pats with Savannah Buffet, and the scripted comedy Olive and Mocha for NBCU digital. DiGa has projects in development with talent such as Ceelo Green, Magician Calen Morelli, Sean Hayes, Mac Lethal, Kristin Cavalari, Kelly Cutrone and Kevin James.


text:
Your browser does not support iframes.
The top-seeded Chiefland softball team was a heavy favorite going into its District 1A-7 semifinal game against fourth-seeded Branford last Tuesday.
A lot was on the line, however, including a program-best fourth straight state playoff berth, to keep the Lady Indians on their toes for any upset bid the Lady Buccaneers might've hoped for.
The Chiefland baseball team opens the District 1A-7 tournament Monday, May 1, at 4 p.m. against Dixie County in Branford.
The winner plays Tuesday against Trenton.
The Bears and the defending champion Indians each finished 4-6 in district play and split their pair of regular season meetings, as CMHS avenged a 10-9 road loss in Cross City with a dominant 11-2 win at home over Dixie County on April 11.
The Chiefland Indians football team held its first official spring practice Monday at C. Doyle McCall Field. The Indians, led by first-year CMHS head man Adam Gore, play at Wildwood on May 19 at 7 p.m. for their spring game.
Six Chiefland sprinters and hurdlers joined the girls' 4x800 relay team in qualifying for the regional championships in track and field at the District 1A-5 meet at Oak Hall School in Gainesville last Wednesday.
Michael Weaver advanced in a pair of events as the Chiefland standout finished second in the boys' 110 meter hurdles (17.87 seconds) and scored third in the boys' 300-meter dash (46.58 seconds).
Please don't call me if your neighbor shoots your dog or poisons your cats. Call law enforcement and if they can't do anything about it, then I can't either. For one thing, I am not getting in the middle of a dispute between neighbors. Second, if I interview one neighbor, then I have to interview the other, which never ends well. Third, we don't have an investigative reporter or other resources.
The run began at Beef O Brady's in Chiefland. The riders then traveled to Akin's BBQ in Bell, Cherry's Seafood & Steak in Trenton, The Mustang Grill and Bar in Archer, Frogs BBQ Pad in Williston and finished at Willard's Restaurant & Lounge in Morriston.
There were over 65 total riders on motorcycles and in cars vying for prizes given to holders of the top seven hands. Also, there was a raffle with over 50 prizes.
The Chiefland Rotary Club's meeting April 19 at the Gathering Table started a little different. Instead of the Rotary Club giving money, it received money.
Two members of the CHS Interact Club, Jessica Ivy and Caleb Hardee presented Skipper Henderson with a check for the Rotary Club. The check represented the investment Chiefland Rotary provided the Interact Club with for a cooler raffle.
Tom Morelock, director of facilities for the College of Central Florida, gave a tour, along with project manager Katie Hunt and builder Todd Duffy, of the new Jack Wilkinson Levy Campus north of Chiefland for the Suwannee Valley Rotary Club last Thursday.
The two-story structure with modern amenities is on schedule for completion this summer, Morelock said, with staff expected to be in place by the end of July.
A ribbon-cutting ceremony is planned for Aug. 11, before classes begin on Aug. 28.
The City Commission held a city budget workshop after adjourning Monday's regular meeting.
City Manager Mary Ellzey presented a budget workshop agenda detailing potential wage increases from the current city employee salary plan.
The city has not upwardly adjusted starting pays since adopting the current plan in 2004. Police and Fire employees have seen upward adjustment independent of the 2004 plan, and are not included in the proposal as a result.
Bull riding seems a little bit like a fraternity of cowboys and bulls that was the original "animal house" long before the movie first appeared in 1978.
Colton Stevens, 23, of Old Town, and Cody Baisden, 28, of Kenansville, have known each other for 10 years.
Stevens is following behind his father and grandfather, who also rode the bucking animals. Baisden, who has been riding consistently since he was 18, said bull riding was just something he always wanted to do.
Both love it.
Copyright © 2017
The Chiefland Citizen is your source for local news, sports, events, and information in Levy County and Chiefland Florida, and the surrounding area.


text:
Paulo Tan & Red
Check out these new shoes by Livie & Luca! A perfect mix of moccasin and loafer, with fun contrast stitching. Each pair is handmade, and made to be lightweight & flexible, designed with developing little feet in mind.
crochet pattern for little loafers
Crochet Pattern that looks like little loafers.
Hand crocheted baby loafs are perfect for little boys who like to relax like Dad! Booties come in a clear gift box!Tags: baby, loafers, booties, baby boys, baby booties, hand crocheted, loafs, baby boy booties, baby boy loafersAdded 7 Years Ago from Jefferiessocks
A Pair of New Shoes is Given to a Child in Need With Every Pair
These shoes will be a staple for your little guy. The classic styling and colors go with everything, dressy or casual. Handmade from leather, each pair has a lightweight flexible sole, perfect for little ones on the go.


text:
Thu Feb 7, 2013, 08:15 AM
Omaha Steve (65,601 posts)
New York expanding nation's biggest transit hub
Source: AP-Excite
NEW YORK (AP) - Sixteen stories below Grand Central Terminal, an army of workers is blasting through bedrock to create a new commuter rail concourse with more floor space than New Orleans' Superdome, just one of three audacious projects going on beneath New York City's streets to expand what's already the nation's biggest mass transit system.
But even with blasting and machinery grinding through the rock day and night, most New Yorkers are blithely unaware of the construction or the eerie underworld that includes a massive, eight-story cavern, miles of tunnels and watery, gravel-filled pits.
"I look at it and I'm in wonder, I'm in awe," says engineer Michael Horodniceanu, president of capital construction for the state Metropolitan Transportation Authority. "I feel like when I went to Rome and entered St. Peter's Basilica for the first time. ... I looked at it and said, 'Wow, how did they do that?'"
In New York, they hauled out so much rocky debris from under Grand Central that it could have covered Central Park almost a foot deep, Horodniceanu says.
FULL story at link.
Read more: http://apnews.excite.com/article/20130207/DA49M3O02.html
In this undated artists rendering provided by the Metropolitan Transportation Authority in New York, the Mezzanine node of the Second Avenue Subway at 46th Street in New York City is shown. The Second Avenue Subway is being built to ease rider congestion on Lexington Avenue trains. (AP Photo/Metropolitan Transportation Authority)
5 replies, 2031 views
Always highlight: 10 newest replies | Replies posted after I mark a forum
Replies to this discussion thread
|5 replies
New York expanding nation's biggest transit hub (Original post)
|Omaha Steve
|Feb 2013
|The Wizard
|Feb 2013
|Feb 2013
|Feb 2013
|Feb 2013
|sir pball
|Feb 2013
Response to Omaha Steve (Original post)
Thu Feb 7, 2013, 09:06 AM
The Wizard (9,080 posts)
1. Really a bomb shelter (NT)
Response to Omaha Steve (Original post)
Thu Feb 7, 2013, 10:23 AM
pscot (20,522 posts)
2. How much water will it hold?
Response to pscot (Reply #2)
Thu Feb 7, 2013, 10:40 AM
eggplant (2,957 posts)
3. it's ok -- it doesn't have ventilation.
apparently they'll just make their own air.
Response to Omaha Steve (Original post)
Thu Feb 7, 2013, 12:12 PM
FailureToCommunicate (8,433 posts)
4. "a massive, eight-story cavern, miles of tunnels and watery, gravel-filled pits."
Sounds great. NOT!
I still like the original one:
Response to FailureToCommunicate (Reply #4)
Thu Feb 7, 2013, 12:34 PM
sir pball (2,576 posts)
5. The sunbeams always look lovely in that image, but..
..they're illuminating the haze of tobacco smoke filling the (cavernous) station. When they cleaned the ceiling years ago, they left one little patch untouched to show how filthy, tarry, gummed-up it was...it's literally black as night. My eyes start to water and I feel a bit nauseous whenever I think too much about what's going on in that pic.


text:
Ski resorts throughout the Northwest are now staying open in summer, using their chairlifts to tote sightseers, hikers, and mountain bikers to the summits. But there's nothing quite like the Oregon Skyway, which debuted last year at Willamette Pass, southeast of Eugene. In spring, roomy eight-person gondolas replace the chairs on the resort's express lift. Climb in and watch the panorama unfold ― Odell Lake, Diamond Peak, and a series of other southern Cascades high points ― as you zip 1,546 feet to the top of Eagle Peak. It's all downhill from here.
Return as you came and go back up later if you like; your ticket's good all day. Or stroll less than a mile east from the top, and you're on the Pacific Crest Trail. Walk south to wind back down to the ski area's base via a string of emeralds known as Rosary Lakes, a total of about 6 miles one way. Or take the PCT northbound to Maiden Peak Trail and follow it down to Gold Lake Road to loop back to the gondola's base. Mountain bikes are prohibited on the PCT but are welcome on several other routes for intermediate or better riders. 9-4 daily; $12, $8 ages 6-16, $1 ages 5 and under. Willamette Pass is off State 58, 69 miles southeast of Eugene and 71 miles southwest of Bend; www.willamettepass.com or 541/345-7669.


text:
Blessed Are They That Mourn (2 of 8) by Daniel Rodgers
This content is part of a series.Blessed Are They That Mourn (2 of 8)
Series: The Sermon on the Mount
Dan Rodgers
Matthew 5:1-12
November 2, 2005
INTRODUCTION: Last week we began our eight-part series on the Beatitudes. The title for each message will come directly from the verses themselves. Our first lesson was; "Blessed Are the Poor In Spirit." This evening, our message is; "Blessed Are They That Mourn." You will notice that each lesson has a blessing and a compliment or a reward; therefore, each outline will have two main points.
Some commentators say that there are two possible meanings here: first, those who are mourning over sin. In (4:17), Jesus said, "Repent, for the kingdom of heaven is at hand." And then, there are those who say it is speaking about affliction and suffering. I personally believe it refers to the latter, those who mourn because of affliction. We are going to consider both thoughts:
A. Mourning Over Sin
1. In 2 Cor 7:10, it says, For godly sorrow worketh repentance to salvation not to be repented of: but the sorrow of the world worketh death.
2. The truth is that we should mourn over sin. Sin, if not dealt with, can affect every aspect of our lives. Sin will keep us from fellowship with Christ; we can lose our joy; God will no longer answer our prayers; and certainly, He will withhold any good thing we hoped that He might do for us. Sin in the life of a Christian is not a good thing.
a. Isaiah 59:2, But your iniquities have separated between you and your God, and your sins have hid his face from you, that he will not hear.
b. Jeremiah 5:25, Your iniquities have turned away these things, and your sins have withholden good things from you.
3. If we find ourselves in sin, we should mourn--we should mourn because we have not only hurt ourselves, but we have hurt God. In James 4:9, we read; Be afflicted, and mourn, and weep: let your laughter be turned to mourning, and your joy to heaviness ...
There are 8098 characters in the full content. This excerpt only shows a 2000 character sample of the full content.
Price: $4.99 or 1 credit
Sign up for a Free Trial with SermonSearch.com and download this sermon free today!
Featured Worship Backgrounds


text:
Warning: You are currently using an unsupported browser -OR- your browser is currently in compatibility mode. Certain features and pages on larimer.org may be impacted or altogether inaccessible. Read More…
|East stairway from third floor
|Third floor restroom plumbing
|Second floor covered food court
|Scaffolding for North side exterior work


text:
Was DIBS a good idea in the first place?
Tiger doesn't like walls, mostly because Tiger enjoys ranging around his territory in the property jungle freely.
But Tiger understands the attraction of owning your own enclosed space with a patch of grass on the side. After all, constantly having to mark your territory in the usual way can get tedious.
Not to mention all the trespassers who keep accidentally dropping their bear traps. Tiger doesn't understand why so many people carry so many bear traps around the jungle.
Anyway, many people dream of owning their own homes and the developer interest-bearing scheme (DIBS) ostensibly helps them to.
To be fair, maybe it does. Cough up the down payment and you have no further financial burden from the purchase until the developer completes your spanking new house, because the developer pays the interest until you get the keys.
That might even motivate the developers to work faster, right? The faster they build your house, the less they have to pay. During that period you only have to worry about paying your monthly rent or your current home's instalments.
But it sounds too good to be true to Tiger. The property industry, like any other business environment, comes down to maximising profit.
According to birds chirping in Tiger's ears, the days of 30-40% profit margins are gone for the developers. If developers actually bear these interest costs then the profit margins will drop further. How will they survive under the pressure for continuous profit growth?
It only makes sense that the 'borne' interest costs are actually priced into the house prices. And that raises transparency questions. Why is DIBS called 'incentive' without informing the buyer that the 'free' cost has been priced in?
Like a salesperson offering discounts after quietly marking up the price, is that not cheating?
In the end this also leads to unnatural inflation of prices. Instead of supply and demand, cost and profits are at work as new launches benchmark on marked-up prices. There are also those who take advantage of the scheme - along with its cousin the 'rebate'- to bet on future appreciation on relatively little or even zero outlay, essentially speculating.
Go to KiniBiz for more .
This TigerTalk article was written by Khairie Hisyam.


text:
Melissa Burkley Personal Information
Melissa Burkley , Ph.D.
Melissa Burkley, Ph.D., 2006, University of North Carolina Chapel Hill.
Dr. Burkley is currently looking for graduate students to join her lab for the Fall 2014 year.
Dr. Burkley teaches undergraduate courses in multicultural psychology (PSYC 4133) and experimental methods (PSYC 3914). She also teaches a graduate course in research design (PSYC 6223). Dr. Burkley teaches courses in research methods and motivation.
Dr. Burkley's research focuses on stereotypes and prejudice. In particular, she is interested in studying the positive and negative consequences of being a member of a stigmatized group. Most recently, she has investigated why women endorse negative gender stereotypes. Her research shows that negative stereotypes are often endorsed after failure in order to protect one's self-esteem. In another line of work, she has helped develop a new measure of implicit attitudes known as the Affect Misattribution Procedure (AMP). This method combines cognitive priming with projective tests in order to create a more valid and user-friendly measure of implict affect, cognition, and prejudice. Other interests include intergroup relations, and social cognition.
Dr. Burkley's research has been featured in Women's Health, Esquire, Cosmopolitan, Marie Claire, and Oprah Radio. Dr. Burkley also writes a blog for Psychology Today entitled "The Social Thinker"


text:
Forgot Password?
Producers click here to register
Click here to become a Guestpert
Books Published By Jacquie Jordan Inc.
Speaking Point: Why did Josh Powell do such a horrible thing to his own young sons? – One of his own sisters recently grew suspicious of him. She actually stated she believed he had killed his wife, Susan. The mayor the town in Utah where Susan disappeared had recently said the $500,000 cost of the town's investigation was worth it because they were getting "hot leads." Josh Powell must have believed that his arrest for murdering his wife was coming soon and didn't want to face that. That maybe explains his own suicide, but not killing his owns sons with a hatchet and fire. This can only be explained by his own extreme narcissism. Narcissism is common trait among criminals in general, and murderers in particular.
Speaking Point: Why did the 9-1-1 operator question the social worker for so long before dispatching police? – The social worker brought the young boys for a supervised court-ordered visit with Josh Powell. Josh Powell immediately locked her out of the house, and the social worker smelled gasoline. It is outrageous that the 9-1-1 operator delayed for so long before dispatching police to the scene. The operator claimed they needed to respond only to life threatening emergencies! What an absolute nightmare for those little boys.
Speaking Point: Why did the court even allow visits between the children and Josh Powell? – Another outrage. You have to wonder what the judge could have been thinking. Josh Powell is suspect number one in the apparent murder of his wife and the judge allows visitation? You are going to allow a potential murderer be with two young boys? Forget about the horrific murder-suicide that came, how about just the thought that the boys would grow up knowing they were forced to be with the person who killed their mother? Unbelievable. Kind the same as the judge who allowed OJ to see his children. How messed up would that make those kids?
Speaking Point: Did Josh Powell's father have a role in killing Susan Powell? – Josh Powell was such a monster, he overshadows what a creep his father is. Powell's father is a child pornographer and voyeur. Especially troubling is that the father apparently had a sexual fixation on his son's wife, Susan. What makes it all the more disturbing is that apparently Josh Powell and his father remained close right up to the end. Could the father have actually killed Susan (perhaps she rebuffed his sexual advances, or perhaps she became aware of his pedophilia and voyeurism and threatened to bring it to light)? Did Josh Powell came up the unbelievable story about the impromptu winter camping trip to cover for his father? At the minimum, it's hard to believe that Josh Powell's father didn't know whether his son ki
Speaking Point: Why didn't the Utah authorities at least try to seek an indictment on Josh Powell? – The prosecutor could have convened a grand jury to investigate Susan Powell's murder. They could have forced witnesses to come testify. They could have forced Powell himself to show up. And if he took the Fifth, the pressure on him would only have increased and maybe they could have gotten a break in the case. The investigators could have put a tail on him practically 24-7, watching for something suspicious and hoping for a break. The evidence on Scott Peterson wasn't much stronger, and he got the death penalty. They should have gone for it.
Related Media: LA Times


text:
Why choose Wagner's Bio-Curcumin?
Curcumin is the active ingredient found in Turmeric root, a traditional medicine with a long history of use in Ayurvedic medicine (a system of traditional medicine native to the Indian continent).
Used for a number of inflammatory conditions, Turmeric is found to be especially beneficial in reducing inflammation associated with joint and digestive symptoms, however needs to be taken in large doses to be beneficial.
Wagner Bio-Curcumin contains Meriva®, a clinically trialled and patented form of Curcumin.
Meriva® Curcumin is shown to be up to 29 times more bioavailable^ and more efficiently absorbed than standard unformulated Curcumin.
This means it is much better absorbed than standard Turmeric or Curcumin extracts.
As a result, you don't need to take as much to benefit from its anti-inflammatory action.
Taken daily, Wagner Bio-Curcumin may help:
* Temporarily relieve pain associated with arthritis and osteoarthritis
* Reduce joint inflammation and swelling, thereby increasing joint mobility and movement
* Relieve symptoms of medically diagnosed irritable bowel syndrome (IBS)
* Maintain healthy cholesterol levels in healthy individuals
^Meriva Curcumin shown to be up to 29 times more bioavailable and more efficiently absorbed than standard unformulated curcumin. Meriva® is a registered trademark of Indena S.p.A.
Customer Reviews
Orders within Australia – Regular Post
Orders will be delivered by Australia Post.
$8.95 for orders weighing less than 3kg
$13.00 for orders weighing more than 3kg
Delivery is FREE if your order is over $149 and weighing less than 3kg.
Extremely heavy parcels may incur additional postage costs. We will contact you prior to dispatch if this is applicable to your order.
Orders within Australia – Express Post
Express Post is available for orders under 500g at a cost of $11.95 and between 501g – 3kg for $19.95. If your order qualifies for Express Post, this will be offered at checkout.
Orders outside Australia
International orders will be dispatched via Airmail. Please allow up to 14 days for international Airmail delivery.
Shipping costs depend on the order weight and destination country. Once you have added the products you require to your shopping basket, select your country from the dropdown list and the shipping cost will be displayed.
Returns Policy
We hope that you will be delighted with your order. However, if for any reason you are not completely satisfied then please contact us within 7 days of receipt of your parcel, and our customer service team will be pleased to assist you with organising a return authorisation. All returned merchandise must arrive back to us in their original, unopened and unused condition.
Payment Options
We accept credit cards and PayPal:
* Visa
* MasterCard
* American Express
* PayPal
For your peace of mind, credit card information does not pass through or stored on our servers. Once you pay for your order, your credit card details are encrypted and processed through the our PCI-Compliant Payment Gateway.


text:
Read the full story here Web Link posted Wednesday, February 19, 2014, 12:00 AM
Town Square
Letter: Hazards of cigarettes, meat are similar
Original post made on Mar 14, 2014
Read the full story here Web Link posted Wednesday, February 19, 2014, 12:00 AM
a resident of Menlo Park: Downtown
on Mar 14, 2014 at 6:45 pm
Peter Lenhardt is a registered user.
Thank you to the Town of Portola Valley!
I'd just like to express my thanks to the Town government and residents for some efforts made on behalf of cyclists and pedestrians during the past year.
Specifically, additional parking enforcement and signage along Portola Road near the Windy Hill parking area has resulted in increased protection for cyclists (and the occasional pedestrian) and prevents us from being forced into traffic lanes by a line of illegally/poorly parked cars.
I've biked in this area for 12 years now, and it is my sense that respect for others and adherence to the law is increasing generally. Efforts by cities and towns in this area are an extremely valuable part of that increasing respect and compliance.
I'll keep doing my part by stopping at signs, yielding to pedestrians, and keeping my lights flashing, but I wanted to share my gratitude and the extra measure of joy I feel when biking through Portola Valley. Your efforts are noted and greatly appreciated.
Peter Lenhardt
a resident of Menlo Park: Downtown
on Mar 14, 2014 at 6:47 pm
Peter Lenhardt is a registered user.
Hmmm, maybe I need to explore this interface a bit further....
a resident of Menlo Park: Suburban Park/Lorelei Manor/Flood Park Triangle
on Jun 4, 2017 at 6:48 pm
Due to repeated violations of our Terms of Use, comments from this poster are automatically removed. Why?


text:
The past month has been controversy ridden for the South African Muslim community, as news of the inauguration of the 'Open Mosque' in Cape Town has forced Muslims to tackle issues of gender equality, sexual orientation and religious freedom.Community radio stations, national broadcasters, newspapers and social media have been abuzz with news of the Open Mosque, covering everything from the protests and backlash against such an idea and death threats against the founder, to the alleged clo … [Read more...]
South Africa's Open Mosque : Media, Feminist Critiques and "Unopen" Mosques.
October 15, 2014 by
Book Review – Sufi Narratives of Intimacy: Ibn Arabi, Gender, and Sexuality
August 21, 2012 by
This review was originally published at Muslim Views.Research in the areas of Islamic and Gender Studies often overlap when it comes to the question of women in the Islamic spiritual tradition. What does Sufism offer to men and women seeking out paths of equality and egalitarianism? How does maleness or femaleness influence spirituality, and is the notion of the un-gendered soul a tenable one in the context of a hyper-gendered legal tradition? Is it possible to go beyond socially instituted … [Read more...]


text:
WATCH: The Coolest Ice Cream Smart Phone App Ever
Häagen-Dazs has come up with a pretty weird reason to buy their ice cream the next time you're at the grocery store.
Recently, they put out a CELL PHONE app, and if you aim your phone's camera at the lid of a pint of their ice cream . . . it makes it look like a hologram of a violin player pops up from the top of it.
And she plays for the exact amount of time you should wait for the ice cream to soften up. Then if you do it with TWO pints, a guy with a cello joins in.


text:
Kingsmen Sweep Singles, Top Chapman
CLU defeats Chapman 8-1
ANAHEIM, Calif. -- The Cal Lutheran men's tennis team swept all six singles matches and improved to 4-1 in the Southern California Intercollegiate Athletic Conference (SCIAC) after defeating Chapman 8-1 Friday. The match marked the first time CLU swept all six singles matches since the season-opener against Occidental on Feb. 8.
The Panthers actually took the No. 1 doubles match as Troy Akin and Brett Buford defeated Alex Nichols and Connor Treacy.
Fortunately for Cal Lutheran, the Kingsmen would find it's winning ways and rattle off seven straight victories including the No. 2 and No. 3 doubles matches. Moises Cardenas and Fergus Scott got CLU its first point of the day when they beat Charlie Werman and Sam Mitteldorf 8-5. Following that, Ian Slater and Jesse Knight gave CLU a lead they would not relinquish, topping Justin Thompson and Evan Valentine 8-1.
Cal Lutheran would keep it going as the match shifted to singles with Cardenas beating Thompson 6-1, 6-3 in the No. 1 matchup and Nichols winning 6-1, 6-2 against Werman in the No. 2.
Treacy gave CLU the match- clinching point when he defeated Buford 6-3, 6-2 in the No. 3 contest while Sean Handley picked up a win in the No. 4 singles match when he rallied after dropping the first set 5-7, but took the next two 6-4, 10-3.
Slater won the No. 5 game 6-4, 6-1 and Scott completed the singles sweep as he defeated Hunter Morris 6-2, 7-5 in the No. 6 contest.
Cal Lutheran (5-8, 4-1 SCIAC) will look to continue its winning ways when it travels to Claremont, Calif. and takes on Pomona-Pitzer. The match is set to get underway at 10:00 a.m.
Box Score: CLU 8 CU 1
CLU Twitter: https://twitter.com/clusports
CLU Facebook: https://www.facebook.com/clusports
CLU Instagram: http://instagram.com/clusports


text:
Disney Dream with Tweens: Disney Dream Cruise Review by mommamd
Overall Member Rating
Disney Dream with Tweens
Destination: Bahamas
Embarkation: Port Canaveral (Orlando)
We spent 4 nights in an All Star Vacation home prior to our cruise. We were close to the Disney Parks, and enjoyed the pool, hot tub, and wi-fi all in our own vacation home. We drove a rented Dollar minivan to Port Canaveral. DH dropped me and the kids and bags at the pier and went to return the van. We were Castaway Club Gold members, but couldnt check in until he showed up 30 minutes late, and were in the 8th boarding group. WE checked in fast and waited for our numbers, More There are too many people in this cruise terminal for this process. There are not enough chairs and 1,000 children running about. Finally we are boarded 40 minutes later and head to the buffet. This is lovely with a huge variety and lovely free soda fountains. We enjoyed the crabs, shrimp, salads. Then we changed into our suits in the restrooms and rode the watercoaster and sampled the pools. WAtercoaster is fun, but lines are long, and the pools are tiny. The hot tubs are kid soup, we headed to the adult hot tub which was relatively empty, as well the adult pool. Stange that it is a through way to to kids side, but ok, no large plants, no decor, no scultures or art, all in all a very plain deck. We expected more theming and magic, it seemed to be built to take a beating.
There are few loungers at the family pools, just chairs. Many kids running about and moms screaming at them into my ear. Hmmm....
Dinner was fun in Animator Palate. Turtles were good. Food was good.
Next night we did the Remy, awesome food, large bill as we got the 75 dollar per person food plus 99 dollar wine pairing. We are a fan of Victoria and Alberts so wanted to repeat that experience. You cannot get a glass of wine, really, the wine list has wines for 13,000 dollars, we didnt want to spend that much. (Ha) The french sommelier is very proud of his list. So much wine and they start you off with a complimentary champagne cocktail, I was too tired to see the fireworks. The kids enjoyed the 3 hours to explore, we sat with them at the Royal Palace restraurant, they played on deck then say the Gnomeo movie in the Walt Disney Theatre. We saw 3 D Tron there as well, which was great, the theatre has an amazing sound system.
DS and DD enjoyed the 2 Disney shows we did see, the first night and the last (Believe) We missed Villains. The queueing was a drag for the shows, it was hot and the door opening was delayed 40 minutes the first night. Crowded and uncomfortable, and free sodas are on the top deck or else you can pay to have soda brought to you to cool you off. No thanks, after we scored our second row seats I went up to get free beverages and brough them down.
The elevators are way too small. The stairs turn into a playgroung after 8 pm, many tweens at the Tween club roaming around and hiding under stairs.
4,00 passengers on our cruise and 1,000 are children.
We didnt stay up late enough to check out the clubs, unfortunately.
3 nights is very short but the price was right for us ;)
We liked Castaway CAy, we did parasailing there, the pelican plunge slide, lunch, and snorkling. The water was too cold for me but DH and the kids enjoyed it. Headed back to the ship at 3 for more watercaoster and Serenity Pool time.
We liked all the goodies our Castaway Gold status gave up, bags, lanyards, rice krispie treats, passport holders, stationary. Cruisers had an offer to save 10 percent on a future cruise, but our dates for fall break were booked up at inside and outside, only balconies available for a large price. We passed.
We had connecting outside porthole cabins, the boy used a pull down bunk with a map of glowing stars above his bed, great Disney magical touch!
Movies on demand, view of the piers, second deck, we were near the Garden restaurant. They offered to get me eggs benedict from another restaurant when I asked about it. I passed, and got it the next day at the buffet.
Waitresses cut our son's meat for him, which is lovely, very family oriented. No prices for cocktail of the day on the dinner menu, our assistant server though 3 dollars but I thought that highly unlikely.
Summary, crowded terminal, too many wild kids. deck spaces uninspired, long lines, small elevators, shows that kids love.
I Less
Published 05/12/11
Cabin review: 9A
Port and Shore Excursions
Read Cruise Critic's Disney Dream Review >>
Ship is really showing her age
Father Son Weekend Away
Worse cruise service ever!
Most awesome customer service,...
A good cruise BUT go on others...
Most awesome customer service,...
Relaxing Caribbean Cruise
A wonderful family trip
Sponsored Links


text:
Where Is Hell Located?
The Bible, which is the only book on earth given to men by our
Creator and Redeemer, has so much to say about the location of Hell.
And no, no matter how bad your life is on this earth, it is not
'hell' on earth, nor is this wicked world the 'only hell there is,'
as some say.
To begin with we want to look at an actual account of a man who went
to Hell given in the Bible. Here it is so you can read it…


text:
Amsterdamse Cello Biënnale
de Paul van Riel
Acerca del libro
An intimate view of the 2nd Amsterdamse Cello Biënnale with its many participants; celebrated soloists and upcoming talent.
For nine days, the Muziekgebouw and Bimhuis in Amsterdam, state of the art contemporary music venues, were alive with the music of Berio, Xenakis, but also (for breakfast !), Bach as well as Beethoven or Stravinski.
Concerts, masterclasses, workshops and the National Cello Concours drew thousands of aficionados, but still, an atmosphere of intimacy prevailed.
A warm and personal reportage by a photographer who is obviously much at home with the subject.
Características y detalles
Apaisado estándar, 25×20 cm
118 Páginas
- Fecha de publicación oct. 29, 2010
- Etiquetas classical music, klassieke muziek, paul van riel, cello, cellist, music, muziek, musician, concert, fotografie, photography, photobook, fotoboek, biennale, stage, people, theatre, theater, culture, cultuur, podium, portret, portrait, portretten, portraits, amsterdam, paulvanriel, cellobiennale
Acerca del creador
Assignment photographer for (international) consumer publications and corporate clients. Autonomous work shown at exhibitions internationally.
Inicia sesión o crea una cuenta para publicar un comentario.


text:
A man with alleged links to an outlawed motor cycle gang had his licence suspended and motorcycle confiscated after he was stopped travelling at more than 60km/h over the legal limit in Yass on Saturday afternoon.
About 5pm police from Goulburn were checking motorists speed on the Hume Highway when they detected a Harley Davidson motorcycle which they say was travelling at 154km/h.
The motorcycle was stopped and the rider, a 26-year-old Yass man, was spoken to by the officers. The police say the rider had a provisional license, which restricts him to a maximum speed of 90km/h. The Harley Davidson was not displaying P-plates, and the engine exceeded the capacity limit, according to the officers.
Police confiscated the motorbike and issued the rider infringement notices for the offences, before suspending his licence on the spot.


text:
Hotel Apostoli Palace
Calle del Padiglion 4702, 30131 Venice, Italy |Show on the map
from ₹ 1,995
- Terms and facilities
- Location
- Opinions
With a stay at Apostoli Palace in Venice (Cannaregio), you'll be minutes from Chiesa Santa Maria dei Miracoli and Rialto Bridge. This inn is within close proximity of Ca' d'Oro and Galleria Giorgio Franchetti alla Ca' d'Oro. Make use of convenient amenities such as wireless Internet access (surcharge), concierge services, and tour/ticket assistance. Make yourself at home in one of the 6 air-conditioned rooms featuring minibars and LED televisions. Complimentary wireless Internet access keeps you connected, and satellite programming is available for your entertainment. Private bathrooms have complimentary toiletries and hair dryers. Conveniences include safes and coffee/tea makers.
Choose your room
Share it
Tripadvisor Score Score based on 340 reviews
Average price per night for this hotel for the following weeks
|23/07/17 - 23/07/17
|24/07/17 - 30/07/17
|31/07/17 - 06/08/17
|07/08/17 - 13/08/17
|₹ 4,957
|₹ 6,365
|₹ 5,813
|₹ 5,182
Services and terms: Hotel Apostoli Palace
Accommodation facilities
- Bar/Café
- Garden/Terrace
- Non-smoker Rooms
- Restaurant
Standard Room Services
- Safe
- Minibar
- Satellite TV
- Radio
- Hairdryer
- Telephone
Nearby points of interest
Reviews: Hotel Apostoli Palace
See more nearby hotels


text:
These authors contributed equally to this work.
This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
We previously reported a gradual increase of relative mitochondrial DNA (mtDNA) copy number during the progression of esophageal squamous cell carcinoma (ESCC). Because mitochondria are the intracellular organelles responsible for ATP production, we investigated the associations among mtDNA copy number, mitochondrial bioenergetic function, tumor invasion and the expression levels of epithelial mesenchymal transition (EMT) markers in a series of seven ESCC cell lines, including 48T, 81T, 146T, TE1, TE2, TE6 and TE9. Among them, TE1 had the highest relative mtDNA copy number of 240.7%. The mRNA of mtDNA-encoded ND1 gene (2.80), succinate-supported oxygen consumption rate (11.21 nmol/min/106 cells), ATP content (10.7 fmol/cell), and the protein level of mitochondrial transcription factor A (TFAM) were the highest and the lactate concentration in the culture medium (3.34 mM) was the lowest in TE1. These findings indicate that TE1 exhibited the highest bioenergetic function of mitochondria. Furthermore, TE1 showed the highest trans-well migration activity of 223.0 cells/field, the highest vimentin but the lowest E-cadherin protein expression levels, which suggest that TE1 had the highest invasion capability. We then conducted a knockdown study using pLKO.1-based lentiviral particles to infect TE1 cells to suppress the expression of TFAM. Molecular analyses of the parental TE1, control TE1-NT and TFAM knockdown TE1-sh-TFAM(97) cells were performed. Interestingly, as compared to the control TE1-NT, TE1-sh-TFAM(97) exhibited lower levels of the relative mtDNA copy number (
Esophageal squamous cell carcinoma (ESCC) is an aggressive neoplasm in Asia, especially in Southern China, Hong Kong and Taiwan [
A complete metabolism of one mole of glucose can generate a maximum of 36–38 mol of ATP, including two moles of ATP after glycolysis in the cytoplasm (oxygen independent) and 34–36 moles of ATP from oxidative phosphorylation in the mitochondria (oxygen dependent). During glycolysis, one mole of glucose can be converted into two moles of pyruvate, which then enters the Krebs cycle for subsequent electron transport and oxidative phosphorylation in the mitochondria to generate ATP. Under some circumstances, when oxygen supply is not sufficient or the mitochondria are impaired, pyruvate is transiently reduced to lactate by lactate dehydrogenase (LDH) in cytoplasm without entering the mitochondria. This kind of glucose metabolism is termed anaerobic glycolysis or lactate fermentation, and only two moles of ATP can be generated by incomplete catabolism of one mole of glucose [
In contrast to normal human tissues, human cancers can display an avid glucose uptake with profound lactate production to generate ATP, even if the surrounding oxygen supply is sufficient. This special phenomenon is termed Warburg effect, which was first observed by Dr. Otto Warburg eight decades ago. He contended that human cancer tissues, unlike normal tissues, obtain an increase of ATP from lactate fermentation in cytoplasm and a decreased reliance on the ATP generated by oxidative phosphorylation in mitochondria. Such kind of metabolic shift in cancer tissues led Warburg to speculate that the function of respiratory enzyme complexes in cancer tissues might be impaired or suppressed [
Generally, there is a mitochondrial network within human cells, and each mitochondrion is connected to other mitochondria during most of the time. However, due to the dynamic processes of fission and fusion of this network, many small mitochondria are disintegrated from the main network that can be observed in different locations at certain moments or at different times inside the cells. This is dynamic and in a particular snapshot, some cells may appear to contain several small mitochondria, besides the un-fragmented mitochondrial reticulum. Only under stress or harsh conditions, especially in a pathological state, the mitochondrial reticulum can be disintegrated in fragments. Generally, each fragment contains at least a single nucleoid and each nucleoid harbors around 1 to 6 mtDNA molecules [
Human mtDNA is a circular and double-stranded DNA with a size of 16,569 bp, including the non-coding and coding regions. The coding region encodes 13 polypeptides that constitute the respiratory enzyme complexes, 2 rRNAs, and a set of 22 tRNAs required for protein synthesis in the mitochondria. The 13 mtDNA-encoded polypeptides include 7 (ND1, ND2, ND3, ND4, ND4L, ND5, and ND6) in Complex I, 1 (cytochrome
During the past decades, biomedical researchers have paid increasing attention to the alterations of relative mtDNA copy number in human cancers. Compared to the corresponding non-cancerous parts, a decrease of the relative mtDNA copy number was found in lung cancer, hepatocellular carcinoma, gastric cancer and breast cancer [
A total of 7 ESCC cell lines, including CE-48T/VGH (48T), CE-81T/VGH (81T), CE-146T/VGH (146T), TE1, TE2, TE6, and TE9 were used to conduct this study [
Besides the relative mtDNA copy number, significant differences were also noted in the succinate-supported oxygen consumption rate (
Regarding the protein expression, as shown in
Most studies have revealed that a high relative mtDNA copy number is associated with a high mitochondrial bioenergetic function in human cells [
It is well established that only 13 polypeptides of the respiratory enzyme complexes are encoded by mtDNA, and all the other peptides are encoded by nDNA. Because the 4 polypeptides of Complex II are totally encoded by nDNA, our finding that TE1 had a relative higher protein expression of SDHA might be caused by the compensatory gene induction. As a result, the high mitochondrial bioenergetic function of TE1 cells might be attributed, at least in part, by the nDNA-encoded polypeptides.
Although TE1 showed the highest bioenergetic function and lowest lactate concentration in the cultured medium, TE1 did not express the lowest level of LDH but a medium one. LDH can convert pyruvate to lactate in cytoplasm to guarantee the execution of glycolysis [
As shown in
Now that TE1 had the highest relative mtDNA copy number and highest mitochondrial bioenergetic function with the highest intracellular ATP content, we were interested to know whether TE1 exhibited a higher migration activity. Significantly, TE1 did express the highest trans-well migration activity. To avoid the confounding effect of cell proliferation to interfere with the analysis of trans-well migration activity, the growth kinetics of the 7 cell lines were also evaluated, and the results showed that the growth rate of TE1 was second to the last. This finding convinced us that there must be a relationship between the bioenergetic function of mitochondria and the migration activity of the ESCC cell line. It is well established that a decrease in epithelial cell marker and an increase in mesenchymal cell marker, the EMT, play an important role in the invasive process of cancers [
Based on the above findings of the 7 ESCC cell lines, we hypothesized that higher relative mtDNA copy number is associated with a higher mitochondrial bioenergetic function and a higher invasiveness of the ESCC cell line. However, such an association needs to be further validated. Thus, we conducted the following gene knockdown experiments.
As shown in
As shown in
As shown in
The above findings in TE1 cells were consistent with the Warburg effect, because the impaired mitochondria could be compensated for by an increase of lactate fermentation to keep up the glycolysis to generate ATP. However, the ATP from the glycolysis seems not enough to make up the ATP from the mitochondrial respiration, because the ATP production of TE1-sh-TFAM(97) was less than that of control TE1-NT. Thus, the growth rate of TE1-sh-TFAM(97) was slightly slower than that of control TE1-NT, but was not significantly arrested. Moreover, the trans-well activity of the TE1-sh-TFAM(97) was significantly decreased when compared to the control TE1-NT cells, and this alteration was much more obvious than the change of growth kinetics. An elevated expression of E-cadherin and decreased expression of vimentin in TE1-sh-TFAM(97) were correlated well with its decrease in trans-well migration activity. These knockdown results further proved our hypothesis that mtDNA copy number is associated with mitochondrial bioenergetic function and invasiveness of ESCC.
When evaluating the relationship between mitochondrial bioenergetic function and cancer, most researchers paid their attention to the Warburg effect and emphasized the mitochondria impairment in cancers [
Regarding the Warburg effect in human cancers, most researchers emphasized the increase in the uptake of glucose to produce ATP. It deserves to mention that when we cultured ESCC, besides the original L-glutamine in the DMEM, an additional 1% L-glutamine was added to the culture medium. Obvious growth retardation occurred if the extra L-glutamine was not added. Since the 1950s, several cancer biologists also noted the importance of L-glutamine as a tumor nutrient. Several novel roles of L-glutamine in cancer biology have been identified, including the essential role of the amino donor in purine biosynthesis, and supply of alpha-keto glutarate to the Krebs cycle and involvement in the production of other macromolecules [
DNA extraction. Approximately 3 × 106 cells were mixed with 500 μL of Tris-EDTA buffer, 50 μL of 10% sodium dodecyl sulfate and 10 μL of proteinase K (20 mg/mL) and incubated at 56 °C for 16 h. Subsequent phenol, phenol/chloroform and chloroform (500 μL) extractions were performed to get the supernatants in three steps and then the DNA was salted out by the addition of isopropanol (500 μL) plus sodium acetate (50 μL) (3 M, pH 5.0) and kept at −20 °C for 16 h. After centrifugation at 12,000
RNA extraction. Approximately 3 × 106 cells were mixed with 500 μL of TRI™ Reagent (Sigma-Aldrich Chemical Co.) plus 100 μL of chloroform to centrifugation at 12,000
Protein extraction. Approximately 3 × 106 cells were suspended in 50 μL of lysis buffer (50 mM Tris-HCl, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, and 1% NP-40, pH 7.4) containing 1% of protease inhibitor (Roche Applied Sciences). The suspension was incubated at 4 °C for 30 min and then centrifuged at 12,000
Quantitative real-time PCR (Q-PCR) using SYBR Green I (Roche Applied Science, Mannheim, Germany) to determine the threshold cycle (Ct) was applied for the quantification of DNA and mRNA, which were described previously [
The mtDNA copy number was defined as total mtDNA copies divided by total nDNA copies. The sequences of primers used for mtDNA amplification (ND1 region) and nDNA amplification (18S rRNA region) were mtF3212: 5′-CACCCAAGAACAGGG TTTGT-3′ and mtR3319: 5′-TGGCCAT GGGTATGTTGTTAA-3′ as well as 18SF1546: 5′-TAGAGGGACAAGTGGCGTTC-3′ and 18SR1650: 5′-CGCTGAGCCAGTCAGTGT-3′, respectively [
The mtDNA encoded mRNA expression was defined as total ND1 copies divided by total 18S rRNA copies. The sequences of primers used for mtDNA encoded ND1 and nDNA encoded 18S rRNA mRNA were ND1F: 5′-TGGGTACAATGAGGAGTAGG-3′ and ND1R: 5′-GGAGTAATCC AGGTCGGT-3′ as well as 18S rRNAF: 5′-CTCAACACGGGAAACCTCAC-3′ and 18S rRNA R: 5′-CGCTCCACCAACTAAGAACG-3′, respectively [
For each reaction, 1 μL of sample DNA (10 ng/μL)/1 μL of cDNA (16× dilution) was amplified in a 10-μL reaction buffer containing 0.25 μL (20 μM) of each primer (mtF and mtR for mtDNA, 18SF and 18SR for nDNA; ND1F and ND1R for mtDNA-encoded mRNA, 18S rRNAF and 18S rRNAR for nDNA-encoded mRNA), 1.2 μL of 3 mM MgCl2, 1 μL of LightCycler SYBR Green I mixed reagent (Roche Applied Science, Mannheim, Germany) and 6.3 μL of PCR grade H2O. Simultaneously, 1 μL of DNA (1 ng/μL)/cDNA (16× dilution) from the 143B cells and PCR grade H2O were included as the positive and negative controls, respectively. The PCR procedures included hot start at 95 °C (10 min) and 40 cycles of 95 °C (20 s), 62 °C (20 s) and 72 °C (20 s). The fluorescence intensity was measured at the end of primer extension at 72 °C for Ct calculation. A melting curve was detected from 55 °C to 95 °C to confirm the PCR product that we interested in. The relative mtDNA copy number and relative mRNA levels of mtDNA-encoded ND1 of each sample were calculated, after adjusting the mtDNA copy number of 143B cell as 100% and the mRNA level of mtDNA-encoded genes of 143B cell as 1.00 by the standard cures we established. Each reaction was done in duplicate and experiments were repeated for 3 independent cultures of cell lines (
The relative protein expression levels were determined by Western blot. An aliquot of 60 μg of total cellular protein was separated on a 10% SDS-PAGE, and then blotted onto a piece of BioTrace™ polyvinylidene difluoride (PVDF) membrane (Pall Corp: Pensacola, FL, USA). Non-specific bindings were blocked with HyCell blocking buffer (HyCell Biotechnology Inc.: South Logan, UT, USA) for 1 h at room temperature. The membrane was subjected to specific primary antibodies against mtTFA (Santa Cruz, sc-19050, 1:500, 25 kD), SDHA (Molecular Probes, A11142, 1:1000, 72.2 kD), lactate dehydrogenase (LDH) (Santa Cruz, sc-33781, 1:5000, 35 kD), E-cadherin (Upstate, 07-697, 1:1000, 106 kD), vimentin (Sigma, V6630, 1:2000, 58 kD) and beta-actin (Chemicon, MAB1501, 1:10,000, 42 kD), and incubated for 16 h at 4 °C. After 4 times of wash by PBST buffers, the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 h at room temperature, the protein band was visualized on an X-ray film (Fujifilm) by using ECL reagents (GE Healthcare, Buckinghamshire, UK) [
The cellular oxygen consumption rate was measured by the 782 Oxygen Meter (Strathkelvin Instruments, Scotland, UK). The reaction was kept at 37 °C with a circulation water system. A total of 106 cells were suspended in a 330-μL assay buffer (125 mM of sucrose, 65 mM of KCl, 2 mM of MgCl2 and 20 mM phosphate buffer, pH 7.2) and transferred into a closed chamber of the oxygen meter to measure the oxygen consumption rate of the cells. After the steady state was achieved, 1 μL of ethanol-purified digitonin (a final concentration of 0.0003%, Sigma-Aldrich) was added to the chamber to permeabilize the plasma membrane, which enabled direct exposure of the respiratory enzyme complexes to the following substrates. An aliquot of 10 μL succinate (1 M) (a final concentration of about 30 mM) was loaded into the chamber for 2 min to measure the succinate-supported oxygen consumption rates (nmol/min/106 cells) [
A total of 106 cells were seeded in 6-well culture plate and incubated for 24 h to achieve the steady state. They were then washed with PBS and replenished with 2 mL of fresh growth medium in 37 °C incubator for 4 h. Ten microliters of the medium was transferred to 96 well plate and mixed with the Lactate Reagent (Trinity Biotech plc., Bray, Ireland). Absorbance at 540 nm was measured on an ELISA reader (PowerWaveX 340), and the value was normalized by the cell number. Each experiment was performed in duplicate and repeated for 3 independent experiments (
The intracellular ATP content was measured by the ATP Bioluminescent Somatic Cell Assay Kit (Sigma-Aldrich) as described previously [
Cell trans-well migration was assayed by using 24-well culture plate and millicell hanging cell culture inserts. The bottom of the insert was covered with a piece of membrane with 8-μm pores (Millipore). A 400-μL of growth media containing DMEM plus 10% FBS was added in the 24-wells, and 5 × 104 cells suspended in 150-μL of growth media containing DMEM plus 1% FBS were seeded in the insert. Followed by 24 h of incubation at 37 °C in a standard cell culture incubator, cells were removed from the upper surface of the membranes of the insert with a cotton swab. Cells that migrated to the lower surface were fixed with methanol for 20 min and stained with 0.2% (
A total of 5000 cells suspended in 100 μL of growth media were seeded on a 96-well microplasty reader plates (Corning Glass Works, Corning, NY, USA). After incubating for 24 (reference point), 48, 72, or 96 h at 37 °C, an additional 10 μL of AlamarBlue™ reagent (Invitrogen) was added to the cells and incubated for 4 h. The fluorescence intensity was measured by the Victor2™ 1420 Multilabel Counter (Perkin-Elmer Life Sciences) on a plate reader at 538 nm (excitation) and 590 nm (emission). The methods for calculation of the cell growth kinetics were described previously by Larson
Small hairpin RNA (shRNA) constructs designed by the National RNAi Core Facility of Academia Sinica in Taiwan (
Initially, the pLKO.1-sh-mtTFA(96), pLKO.1-sh-mtTFA(97) and pLKO.1-NT were transformed to DH5α strain of
About 1.5 × 106 of TE1 cells were suspended in 3 mL of growth medium and were seeded in a 6 cm culture plate. After 24 h of incubation, the growth medium was changed to a fresh one containing polybrene (8 μg/L) and sufficient amount of virus particles (2 multiplicity of infection, 2 MOI) were added for virus infection. Twenty-four hours later, the medium was changed to a new one containing puromycin (2 μg/mL) for clone selection.
After virus infection, 3 new clones were established. TE1 cells harboring pLKO.1-NT after 2 MOI of virus infection were named as TE1-NT. TE1 cells harboring pLKO.1-sh-TFAM(96) after 2 MOI of virus infection were named as TE1-sh-TFAM(96). TE1 cells harboring pLKO.1-sh-TFAM(97) after 2 MOI of virus infection were named as TE1-sh-TFAM(97).
The continuous variables among different cell lines were compared using analysis of variance (ANOVA) and between 2 cell lines were compared using student
In conclusion, our experimental results showed that a high copy number of mtDNA may contribute to the high bioenergetic function of mitochondria and further confer an advantage for tumor invasion in ESCC cell lines.
This work was supported by grants from the Department of Health, ExecutiveYuan, Taiwan for Taipei Hospital (DOH 99-05) and National Science Council, Taiwan (NSC 100-2314-B-192-001).
Western blot analysis shows that TE1 had the highest mitochondrial transcription factor A (TFAM) (
Illustration of the quantitative (bar, cells/field) and photographic (light microscopy) results of the assay of trans-well migration activity among the seven cell lines. TE1 exhibited the highest trans-well migration activity.
Growth kinetics of the seven ESCC cell lines. Data are shown in percentage when the cell number on day 1 is defined as 100.0%. The graph was plotted by using the SPSS 12.0 software (SPSS Inc, Chicago, IL, USA).
Comparison of the relative mtDNA copy number, bioenergetic function and invasion activity of seven esophageal squamous cell carcinoma (ESCC) cell lines.
|Cell lines
|Biological variables
|Relative mtDNA copy number (%)
|108.9 ± 43.8
|79.4 ± 3.5
|79.7 ± 7.9
|240.7 ± 54.2
|73.2 ± 14.0
|98.0 ± 42.9
|121.8 ± 26.0
|Relative mRNA expression level
|ND1 (mtDNA encoded ND1 gene)
|1.71 ± 0.29
|0.74 ± 0.32
|0.91 ± 0.30
|2.80 ± 0.73
|0.75 ± 0.20
|0.53 ± 0.16
|1.65 ± 0.59
|Oxygen consumption (nmol/min/106 cells)
|6.86 ± 0.56
|10.03 ± 0.16
|10.22 ± 1.10
|11.21 ± 0.11
|8.10 ± 0.69
|10.52 ± 0.82
|8.18 ± 0.41
|ATP content (fmol/cell)
|8.10 ± 0.36
|8.50 ± 0.40
|5.57 ± 0.21
|10.67 ± 0.51
|7.17 ± 0.29
|5.20 ± 0.10
|6.50 ± 0.30
|Lactate concentration (mM)
|4.02 ± 0.03
|4.79 ± 0.10
|4.06 ± 0.27
|3.34 ± 0.05
|3.58 ± 0.12
|4.37 ± 0.18
|6.29 ± 0.29
|Transwell migration (cells/field)
|54.7 ± 2.3
|41.7 ± 3.5
|5.7 ± 1.5
|223.0 ± 15.9
|3.7 ± 1.5
|4.0 ± 1.0
|103.0 ± 12.3
The relative mtDNA copy number of 143B cell is defined as 1.00 (100%);
The relative ND1 mRNA expression level of 143B cell is defined as 1.00.
Comparison of the relative mtDNA copy number, bioenergetic function and invasion activity of the parental TE1, control TE1-NT and TFAM knockdown TE1-sh-TFAM(97) cell lines.
|Biological variables
|Cell line
|Relative mtDNA copy number (%)
|221.7 ± 9.8
|227.8 ± 5.1
|152.5 ± 13.4
|Relative mRNA expression levels
|ND1 (mtDNA encoded ND1 gene)
|3.12 ± 1.04
|2.90 ± 1.13
|1.70 ± 0.11
|Oxygen consumption (nmol/min/106 cells)
|8.36 ± 0.94
|8.66 ± 1.16
|6.36 ± 1.07
|ATP content (fmol/cell)
|10.13 ± 0.67
|9.90 ± 0.44
|7.27 ± 0.76
|Lactate concentration (mM)
|3.39 ± 0.19
|3.36 ± 0.12
|4.31 ± 0.34
|Trans-well migration (cells/field)
|230.7 ± 9.6
|224.7 ± 6.5
|95.7 ± 7.2
The relative mtDNA copy number of 143B cells was defined as 100.0%;
The relative ND1 mRNA expression level of 143B cells was defined as 1.00.