text:
Students to help speedway company build audience
Getting millennials out of their living rooms and into NASCAR speedways is the goal of one of four projects being undertaken by the Vanderbilt Accelerator Summer Business Institute.
Media Contact:
Jim Patterson
Public Affairs Officer
Vanderbilt University
(615) 322-NEWS | email@example.com
Jun 4, 2014
Getting millennials out of their living rooms and into NASCAR speedways is the goal of one of four projects being undertaken by the Vanderbilt Accelerator Summer Business Institute, an annual business boot camp run by Vanderbilt Owen Graduate School of Management.
Students enrolled in the institute will consult with the International Speedway Corporation (ISC), which runs 13 speedways, including Daytona and Talladega, and has nearly 900,000 grandstand seats and 500 suites to fill during races, mostly NASCAR events. It's the kind of business challenge that Accelerator students, who are college students or recent graduates, like to immerse themselves in and work to create winning solutions.
Accelerator Summer Business Institute helps students hone essential skills in marketing, sales, finance, real estate, research and corporate strategy, while participating companies receive the brainpower, creativity and proposals from several teams of highly motivated students.
The ISC project launch is 8 a.m. June 16 in Averbuch Auditorium in Owen Graduate School of Management, 401 21st Ave. S. Final presentations will be made at the same location at 8 a.m. June 23. To attend the ISC or other Accelerator presentations, contact Jim Patterson.
Other Accelerator 2014 projects include:
- Executives of The Cosmetic Market, a beauty supplies and aesthetic services retailer, want students to optimize the in-store experience at its various locations while increasing profits for the company. The company operates four stores in the Nashville area and is opening a new location near Atlanta in the fall. The new store is the focus of the challenge, but successful ideas will be implemented in all locations.
- Using Dollar General as a case study, student teams will perform a quantitative analysis and deliver an equity analyst report, providing qualitative and quantitative justification for their 12-month target stock price.
- Griffin Technology executives wish to develop a multi-device charger that falls between its PowerDock 5 and its MultiDock. They want the student teams to define audience segments, desired price point and the must-have functionality for a multi-device charger in this range.
More information about Accelerator is available online. To follow the progress of Accelerator students and their projects, visit the Vanderbilt Accelerator Summer Business Institute page on Facebook.
Vanderbilt Owen Graduate School of Management is ranked as a top institution by BusinessWeek, U.S. News & World Report, Financial Times and Forbes. For more information about Owen, visit owen.vanderbilt.edu or follow us on Facebook, Twitter, LinkedIn or Instagram.


text:
:: Financial Accounting
Fall 2010
@Leeward CC
|Credit Hours:
Tina Lee
455-0349 (Ph)
453-6735 (Fax)
|Course URL:
|Instructional Mode:
|08/23/2010 - 12/09/2010
|Course Requirements:
|"Students must log into Laulima for instructions on how to get started with the class. If you do not see the course in your Laulima account, please contact the instructor imediately."
|Course Prerequisites:
|ENG 21 or ENG 22, and MATH 1B with a grade of C or better or equivalent.
|Course Description:
|Introduction to accounting and financial accounting theory including: Accounting as an information system; the accounting cycles; accounting for merchandising operations; financial statements and internal control; short-term liquid assets; inventories; current liabilities, payroll, and long-term assets.
|1.Kimmel, Weygandt, and Kieso, Accounting: Tools for Business Decision Making, 3rd edition, Volume 1. Publisher: Wiley & Sons, Inc.
2.WileyPLUS online homework manager access code (to accompany the text)
3.Calculator, straight edge, #2 pencils, eraser, pen
Books may be purchased at the Leeward CC bookstore or ordered online at http://www.bookstore.hawaii.edu/lcc/home.aspx. If you do not have access to the Internet or unable to travel to the bookstore, you may also order by phone at 1-800-UHBOOKS
|Technical Requirements:
|The Internet allows students to take a course at home using a personal computer. Familiarity with using a computer and basic Internet skills (browsing, sending e-mail, saving/sending files) is required. Students must have a computer with Internet access. Depending on the online course, students may be required to log in at a specific day and time or at anytime. Students taking an online course should always contact their instructor after registration or prior to the first day of class to get detailed information regarding the course.
Minimum Computer Hardware/Software Requirements:
It is also required that you obtain a UH Email account. Please go to the following URL for more information: http://www.hawaii.edu/askus/563
Laulima Help
|Additional Notes:
|Alternate Technology Access Plan: In registering for a Distance Education course (Cable or Web) the student is responsible for finding immediate alternate access to a computer with Internet connectivity or cable television should that student experience technical difficulties. Technical difficulties can include but are not limited to problems with a student's computer hardware/software; inoperability of a student's VCR or DVR; or lack of service by a student's Internet Service Provider (ISP) or Cable Provider. Technical issues do not constitute the extension of an assignment, project, quiz or exam deadline unless agreed upon by the instructor.
An Alternate Technology Access Plan will be made by the student prior to the start of the semester and should be implemented immediately upon encountering technical difficulties. The student is required to continue course work as a result of having an alternate plan of access while independently resolving any technical issues with hardware/software, VCR/DVD, ISP, or Cable Provider.
|Tuesday, April 20, 2010 12:39:49

subset: commoncrawl


text: Cook honored for entrepreneurship education Posted: March 07, 2011 Author: Meaghan GordonPrevious1 of 1NextLIVINGSTON, Ala.—A University of West Alabama assistant professor recently received the 2011 College Entrepreneurial Educator of the Year award from the University of Alabama for his contributions in the classroom and the Black Belt. Charlie Cook, a 20-year UWA employee, has taught entrepreneurial classes and led the UWA Small Business Development Center. He and four others from across the state were honored at a ceremony and reception in the North Zone at Bryant-Denny Stadium. "Mr. Cook is very deserving of this recognition due in part to his many years of dedicated service to all things entrepreneurial, from teaching and inspiring students in entrepreneurial classes to engaging communities and businesses in strategic, comprehensive economic development planning," said Dr. Ken Tucker, UWA College of Business dean, who nominated Cook. Cook has encouraged and supported students in starting their own businesses. He says former students now contribute to the economic health of their communities through their business ventures in home building and construction, restaurants, automobile dealerships, accounting practices, marinas, art and fashion boutiques and more. As director of the UWA Small Business Development Center, Cook developed a simplified business plan format that was adopted for statewide use by beginning entrepreneurs to define and monitor their progress on requirements critical to the initial success of their entrepreneurial endeavors. He has also worked closely with officials in the University's service area to prepare comprehensive development plans for their communities. Cook, a Sam Walton Fellow, served as faculty advisor to UWA's Students in Free Enterprise chapter and Delta Sigma Pi professional business fraternity. He has also assisted with UWA's online program through set up and trouble-shooting. Although he will retire in May, Cook maintains an active private consulting practice to advise local businesspeople and provides many hours of pro bono advisory and technical support services to public and private entities. See More UWA News


text: Hi, All I am new to these forums but found the information here very useful. I have just recently built a Mac Mini Home theater from the advice from this forum and its been working very well except so some movies (downloaded MKV files) play fine on "Windows Media Player classic" but don't play properly on Plex, XMBC or VLC. If you forward the file by 1-2 min it starts playing fine but otherwise the player just stops playing the file after 1-2 seconds. Its seems to be a issue with the file for sure, but some player/CODEC seem to be more robust in handling glitchy files than others. This also only seems to happen only in the beginning of the movie, once you forward, say 1 min they seem to play fine. Same behavior happens if I use WD Live but the same file works fine with Popcorn Hour. It seems like the beginning of these files is partially corrupted. Any suggestions on how to fix this? other than downloading the file again? are there any codec's i can change in VLC or Plex which can address this? or are there any utilities i can run on the MKV files to clean the glitch out? Appreciate your feedback.


text:
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Lady Gaga – Scene from Poker Face & Paparazzi videos
Feb 2013
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© 2011-2017 CakesDecor.com. All Rights Reserved.
DISCLAIMER: Any posts on CakesDecor.com (CD) are posted by individuals acting in their own right and do not necessarily reflect the views of CD. CD will not be held liable for the actions of any user.


text:
I know the title is lame, but it's also true. Sometimes life is like that. Let me give you a run-down on what the Campus Challenge is and you can decide if you agree with me.
You know all those nifty apps that you use on a daily – even hourly – basis on your smartphone? Well they all have to be developed by somebody. TradeKing is an online broker that is challenging students to become the "somebodies" who develop those apps with a contest called Campus Challenge. They tried this program at Loyola University Maryland during the spring of this year, and now they've rolled it out nationwide – this time with $100,000 in prizes available!
The idea is fairly simple: Develop an app that runs on any platform, designed in any language, which utilizes the Tradeking.com application programming interface (API) either alone or in combination with other APIs from Facebook, Twitter, Yahoo Finance, etc. In other words, build a better online or mobile investment "mousetrap." Oh, and did I mention that the contest is only open to students?
That part alone is already pretty cool. I always love the idea of teams competing to solve a problem in the best way possible. Not only does each team learn a lot in the process, but the awareness that they are competing with other teams drives each group to perform to the best of their abilities. Some really amazing stuff comes from contests like this.
But what makes Campus Challenge "way cool" to me is how my alma mater, Loyola University Maryland is involved. It's one of only five schools (out of the 30 teams that have entered) that is weaving the contest right into the classroom instead of setting up extra-curricular teams. That mean students are doing this as their class work, not in addition to it.
This, to me, is the future of education. Why should it be that students create nothing of value to the world outside of their university until after they've graduated? It's a totally artificial barrier. In fact, why aren't we challenging students to contribute to the "real world" right from the beginning?
I see contests and opportunities like this as a great step towards that happening. The face of education is changing, and it is all thanks to schools being willing to step outside of what is considered "normal" and companies like TradeKing who believe that students can make a difference too.
Check out the Campus Challenge website here. If it sounds intriguing to you, see what you can do to get your school to participate in a contest like this one next year!


text:
Gotham Girls Roller Derby All-Stars win Hydra Trophy at WFTDA Championships
Gotham Girls Roller Derby All-Stars celebrate with the WFTDA Hydra Trophy on Sunday at 1st Bank Center in Broomfield
Sarah Austin
New York's Gotham Girls Roller Derby All-Stars were the big winners at the 2011 Women's Flat Track Derby Association (WFTDA) Championships at the 1st Bank Center this weekend, beating out West Region champs the Oly Rollers Cosa Nostra Donnas, 140-97. Gotham Girls also trounced the Rocky Mountain Rollergirls 5280 Fight Club -- local favorites and defending champs -- and Austin's Texecutioners on the way to winning the Hydra Trophy.
Gotham Girls heading into battle against the Oly Rollers, from Olympia, WA.
Sarah Austin
The Rocky Mountain Rollergirls 5280 Fight Club won its opening bout on Friday, dispatching Nashville's Music City All-Stars 198-58, but lost to Gotham 187-134 on Saturday. Despite ousting the local heroes, Gotham became the clear fan favorite from that point on: The Oly Rollers, 2009 WFTDA champs and last year's second-place finishers, have a long history of dominating the flat track derby circuit, and the crowd at the 1st Bank Center seemed primed to root against them.
Semi-finals on Saturday ended up being quite a smash-up, with Gotham beating the Texecutioners 195-113 (the dude in the crowd wielding a chainsaw and shouting "Kill! Kill! Kill!" sure was pissed) and Oly rolling over the the Kansas City Roller Warriors 124-104 (though we'll give the Warriors' "Kansass City" booty shorts the win for the weekend's best uniform touch). Texas went on to beat Kansas for third place in Sunday's consolation round.
Gotham Girls and Oly Rollers each came into the WFTDA Championships undefeated in 2011 competition
Sarah Austin
Sunday's finals were the match-up most roller derby fans had predicted: The Gotham Girls came into the tournament as the East Region champions, after an undefeated season. The Oly Rollers came in as the West Region champions (they beat Rocky Mountain Rollergirls in the Bridgetown Brawl West Region Playoffs last month), also capping off an undefeated season.
Penalties in the final bout helped shift the balance to Gotham Girls
Sarah Austin
Fans looking for a fight got what they came for: Oly Rollers skaters spent a total of 48 minutes in the penalty box, to Gotham's 37 minutes, and two of Oly's top skaters, Sassy and Tannibal Lector, were ejected from the game. That helped shift the balance to Gotham, pouncing on power plays to run the score up, for a final score of 140-97.
Hydra, we hardly knew 'ya. Rocky Mountain Rollergirls, 2010 WFTDA Champs, relinquish the Hydra Trophy to the Gotham Girls Roller Derby All-Stars
Sarah Austin
Upcoming Events
Deray Davis
TicketsSat., Aug. 26, 7:00pm
Cindy Kaza with Andy Byng!
TicketsWed., Aug. 30, 7:30pm
Jeff Dye
TicketsThu., Aug. 31, 7:30pm
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TicketsFri., Sep. 8, 7:30pm
Joe Torry
TicketsThu., Sep. 14, 7:30pm
Next year's WFTDA Championship tournament will be hosted by the Atlanta Rollergirls, November 2-4, 2012.
Local fans will have two more chances to catch some roller derby action this season: The Rocky Mountain Rollergirls will host an Air Force vs. Army Mixup Bout at the RMRG War*house, 5200 Smith Road, on November 19, as well as a holiday-themed bout -- Kill Scouts' Grinches vs. the Ho Ho Hoes -- on December 10.
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text: 9/25/12 9/25/12 POPSUGAR Celebrity Anna Camp Pitch Perfect Premiere in LA | Pictures Elizabeth, Rebel, and Anna Premiere Pitch Perfect With Smiles and Laughs September 25, 2012 by Lauren Turner 20 Shares Chat with us on Facebook Messenger. Learn what's trending across POPSUGAR. View on One Page Photo 3 of 35 Start Slideshow ADVERTISEMENT () Share This Link Copy ← Use Arrows Keys → 3 Previous Next Anna CampAdam DeVineRebel WilsonPitch PerfectMax HandelmanAnna KendrickElizabeth Banks


text:
M3 steering wheel lower panel install help!
I just bought a M3 steering wheel without the upper (multifunction button) panel and the lower ("T" shape) panel. I will be transfering my existing sport steering wheel upper (multifunction buttons) panel to the M3 steering wheel. I have also purchased the M3 lower panel cover from the dealer because the M3 one is solid versus the "split" spoke my sport steering wheel has.
My question is this. I noticed on the sport steering wheel the lower spoke is fastened to the steering wheel by a couple of screws from the back like this http://www.my330i.com/photos/mod4h.jpg
However with the M3 steering wheel with a solid lower spoke, there aren't any holes in the back to put the screws in! How am I suppose to fasten the cover??
Please help
I just installed my M3 wheel yesterday, the lower panel screws in exactly the same as the old forked sports one ... just dont me an idiot like me and forget to plug the wires back into the slip ring after you recconect the airbag :(
so you are saying there are a couple holes in the back of the steering wheel where it allows me to feed the screws in? Reason I am asking is because I had seen pics of the M3 steering wheel in several ebay auctions and there are no holes in the back that I can see. Could you show me a picture of the back of your steering wheel?
Yah sure I will take some pics after i get out of class tommrow ~3-4pm west coast time if no one else posts by then, but yah it should have the exact same holes on the back as the sport wheel.
1 Attachment(s)
oh ... just realized that i could 'borrow" the pic from the auction i won it from
there at the bottom you can see where the holes are hope this helps ... youll love the wheel once you get it in :)
excellent!! thanks very much.
I realized the picture I was looking at in the other ebay auctions were of the horizontal spoke, not the bottom one!
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text:
The Shakespeare Conference: SHK 11.1625 Thursday, 31 August 2000.
Date: Wednesday, 30 Aug 2000 15:20:31 -0300
Subject: Stratford Ontario 2001 Season
SHAKSPERians may be interested in the announcement of Stratford
Ontario's 2001 season
August 28, 2000 . . . . Stratford Festival Artistic Director Richard
Monette today announced the Festival's 2001 playbill, which will feature
14 productions running in repertory over six months. It will be the
Festival's biggest season in 10 years.
"A number of goals have converged in shaping this season's playbill,"
said Mr. Monette. "First, it fulfills a long-held ambition of mine to
mount a cycle of Shakespeare's history plays in the same season - Henry
IV, Parts 1 and 2, and Henry V - so that patrons can experience these
great dramas of power and personality either as individual stories or as
part of a grand and sweeping epic saga. The playbill also contains one
of Shakespeare's very greatest comedies, Twelfth Night, and one of his
most challenging and controversial 'problem' plays, The Merchant of


text:
Erin Oberly on Jul 14, 2017
cavgirl2002 on Jul 1, 2017
Great print, brings back happy memories of Cougar Gold. Thanks so much!
cavgirl2002 on Jul 1, 2017
Lovely print. It's hanging on my wall as we speak. Thanks so much!
Susie Hooper on Jun 14, 2017
Love this print. Very quick dispatch and sturdy packaging. Thank you
Egg tempera still life originals and giclée prints
11 years ago I finished university in New Zealand and moved to the United Kingdom. Here I worked as a graphic designer until 4 years ago when I began painting. I had entered a painting in a local art exhibition and from this I was offered an exhibition at a gallery were I live in Liverpool. I put the work from this show online and from there it has gone well, so well that now I just paint.
I paint primarily in egg tempera and enjoy the technical control this allows me while preparing my own paints and grounds.
The cultural transition I experienced early in my career has influenced my subjects which often focus on aspects of British life. I paint mainly still-life, depicting everyday objects in an intimate, isolated and sometimes photographic way. This meticulous, graphic representation of objects I attribute to my background in graphic design. The objects, which range from shoe polish to polos and pork pies, are set against neutral backgrounds. With no context given the interpretation of the works remains open, but subjective readings are unavoidable. I enjoy small, often unobserved details and imperfections; spills, stains, or a scrape on the edge of a shoe polish tin. Food has been the inspiration for much of my work, I like that it connects with a wide audience as we all experience food. It is a fun, cheeky subject that often makes people smile.
My paintings were commissioned for the book 'The Taste of America' published by Phaidon in 2013. I have exhibited her work in both solo and group shows, including the Summer Exhibition at the Royal Academy of Arts, London 2012.
Shop members
Joël Penkman
I am a New Zealand–British painter who currently lives and works in the UK.
Shop policies
Accepted payment methods
- Accepts Etsy Gift Cards and Etsy Credits
Prints will be posted First Class to UK addresses (1-2 working days) and International Airmail to overseas addresses (5-7 working days but can occasionally this can take an additional 25 working days). Please note if you are ordering from outside the United Kingdom it takes a minimum of 2-3 weeks and up to 2 months for packages to arrive and this is out of my control.
Small original works will be sent Royal Mail with extra insurance, Special delivery (UK addresses, 1 working day) and International Signed For (overseas addresses, 5-7 working days but can occasionally take an additional 25 working days). Large original works will be sent by a courier service with full insurance.
I do not take responsibility for any duties and customs charges that might be incurred.
Refunds and Exchanges
If you change your mind that's ok to. Let me know as soon as you can and on the safe return of any item I will refund the cost of the item but unfortunately not the postage (you'll need to cover postage both ways).


text:
-Established 1997-
page added: 9/21/07
Hi-Def Reviews
Blu-ray Disc reviews by
Greg Suarez
The Digital Bits
The Replacement Killers: Extended Cut
1998 (2007) - Sony Pictures Home Entertainment
Released on Blu-ray Disc on September 11th, 2007
Film: C+
Video (1-20): 15
Audio (Uncompressed PCM - 1-20): 18
Extras: B
Specs and Features:
96 mins, Not Rated, MPEG4 AVC 1080p widescreen (2.40:1), BD-25 SL, Elite Blue HD packaging, "making of" featurette,
Chow Yun-Fat Goes Hollywood
featurette, animated film-themed root menu with audio/"in-film" menu overlay, scene access (16 chapters), languages: Uncompressed PCM 5.1 (English and Italian) & Dolby Digital 5.1 (English, French, Italian and Hungarian), subtitles: English, Spanish, French, Italian, Portuguese, Chinese, Mandarin, Korean, Thai, Hebrew, Arabic, Dutch, Swedish, Hungarian, Czech, Norwegian, Danish, Hindi, Polish, Greek, Icelandic, Finnish, Turkish, Slovenian, Bulgarian, Croatian and Romanian, Closed Captioned
Sometimes after a stressful day at the office all I want to do is come home, fire up my home theater, switch off my brain and watch some good old-fashioned excessive violence.
The Replacement Killers
fills that need quite nicely. This movie is wholly predictable, filled with clichés and has a paper-thin plot. But for some reason it's a flick I still find entertaining. If you feel the same way read on to find out if an upgrade to Blu-ray is worth your money.
Powerful Chinatown crime boss Mr. Wei calls on his top assassin John Lee (played by Chow Yun-Fat, the Patron Saint of Pistol-Wielding Bad Asses) to avenge his son's death at the hands of police detective Zedkov (Michael Rooker). When John's conscience won't allow him to carry out the execution he must flee the wrath of Boss Wei and high-tail it to China to protect his mother and sister lest Wei finds them first and makes them pay for his disobedience. John tracks down expert document forger Meg Coburn (Mira Sorvino) to hustle together a fake passport. But Wei's thugs follow John to her office and the feisty, take-no-guff Meg becomes embroiled in the violence and takes on the role of John's unlikely ally. Wei hires a pair of brutal replacement killers (one of whom is real-life bad ass Danny Trejo) to finish John's assignment and to finish John himself. John and Meg decide to strike back at Wei and his army of villains and make a stand for what's right. But will they be successful in the face of insurmountable odds?
The Replacement Killers
is almost so by-the-book as to be another forgettable entry in the pantheon of action cinema, the film's saving grace is its charismatic heroes. Chow Yun-Fat is always the strongest asset of gritty crime tales such as
The Corruptor
The Killer
and (of course)
. These modern-day pulp diversions are showpieces for his commanding screen presence and addictive charisma. Casting Yun-Fat in an otherwise mediocre film like
The Replacement Killers
helps make the experience more memorable.
The biggest treat this film provided for me was how well Mira Sorvino performed in her first (and, I believe, only) role as an action heroine. Sorvino's hard-edged physical and emotional presences in
The Replacement Killers
not only complemented Yun-Fat's character, but were also able to hold up against his commanding performance. While I don't want to give the impression that these were Oscar-worthy turns, in the genre of action cinema if a director casts a couple of wet noodles as his heroes, he's well and truly screwed before the first frame of film is shot. That is definitely not the case here. Antoine Fuqua proved in his directorial debut that he had a keen eye for casting this style of film.
The 2.40:1 MPEG4 AVC transfer on the Blu-ray edition of
The Replacement Killers
is good but not great. During optimal, brighter conditions the transfer can look very nice, sporting crisp detail. But overall the transfer has slightly overblown contrast and vivid colors appear pasty. Plus there is a lot of grain in this transfer a lot! Normally grain doesn't bother me because it is a natural part of watching film. However, on this BRD the level of grain washes out finer detail in dark scenes, and there are a lot of dark scenes in this film. While the transfer definitely is not the best there is on the Blu-ray format, it is still a hands-down improvement over the standard DVD version of the film.
And then you have the audio. The uncompressed PCM 5.1 track is sublime. The movie's ample amount of gunfire is potent and sweetly punctuated with kick from the subwoofer. During the many action sequences the entire soundstage explodes with the chaos of a firefight bombarding the listener from every direction. During subtler moments dialog is always clear and well mixed. On the downside, during these quieter moments ambience is sacrificed for the score, which can be a touch over-mixed, drowning out environmental effects.
The extra features included on
The Replacement Killers
Blu-ray are sparse and not really worth your time. There's a short "making of" featurette that's purely promotional and lacks anything in the way of insight. The longer featurette called
Chow Yun-Fat Goes Hollywood
is basically a 20-minute love letter to Yun-Fat from acting and directing collaborators and video store clerks (?). On the brighter side, the extended cut of the film appears on this Blu-ray release. The extra 10 minutes or so added to this cut provide a bit more character depth and is the preferred version of the movie.
So is
The Replacement Killers
worth the upgrade to Blu-ray? Yeah, I'd say go for it: Even though the transfer has its faults it's still better than the standard DVD and if your home theater is capable of playing PCM surround audio you will not be disappointed by the rat-a-tat coming from your speakers.
Greg Suarez
[email protected]
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text:
Newbury Racecourse held its first race in 1905 and has long been one of Britain's leading tracks, staging up to 30 days of top class Jump and Flat racing annually, with at least one raceday in every month of the year.
The highlight of Newbury's Jump racing programme is the Hennessy Gold Cup, one of the most prestigious and sought after prizes in the sport and the focal point of the bet365 Festival in late November.
On the Flat, Newbury's high-quality programme includes the Al Shaqab Lockinge Stakes in May, with the Group One race over a mile worth £350,000. It has been won by many of the greats of the sport, including Frankel in 2012.
Get the Best Seats and enjoy the Greatest Racing Day Find out more
Get the Best Seats and enjoy the Greatest Racing Day Find out more
Newbury Racecourse, Newbury, Berkshire, RG14 7NZ Map
Contact the Racecourse Find out more
See the results for the last meeting Find out more
predictor strike rate at this course
In the last 1 month at Newbury, our top prediction for each race


text:
Biotechnology: Products of Secondary Metabolism, Volume 7, Second Edition
Copyright © 1997 VCH Verlagsgesellschaft mbH
Editor(s): H.-J. Rehm, G. Reed
Published Online: 28 MAY 2008
Print ISBN: 9783527283170
Online ISBN: 9783527620890
About this Book
Products of secondary metabolism from fermentation and cell culture are mainly pharmaceuticals, ranging from traditional antibacterials like beta-lactams, new generation compounds like dalbaheptides and lantibiotics to immunomodulators and antitumor drugs. The major fields of application of these low volume/high price products are human and veterinary medicine as well as plant protection. In this volume functionally based screening approaches and the applications of molecular genetics in the discovery and process improvement of compounds are emphasized. Along with the exploitation of new sources the growing understanding of biosynthetic processes permits the identification of the genetic backgrounds.
Topics included are: Novel Receptor-Active Compounds - Microbial Lipids - Microbial Siderophores - beta-Lactam Antibiotics - Peptide Antibiotics - Lantibiotics - Glycopeptide Antibiotics (Dalbaheptides) - Aminoglycosides - Products from Basidiomycetes - Cyclosporins - Secondary Products from Plant Cells - Antitumor Agents


text:
Spirits and Libations of Hawaii
Tropical Drink Recipes
by Kohana Au
About the Book
Originally the partaking of fermented libations was confined to the priests and chiefs as part of their rituals, a custom that appears to have prevailed in all early civilizations. In later years a fermented sweet potato juice was used by the common folk. Apparently there was not a particular ceremony connected with its use, but the early Hawaiians reserved it for special occasions.
Features & Details
- Category Cooking
Small Square, 7×7 in, 18×18 cm
36 Pages
- Publish Date May 27, 2010
- Tags Hawaiian Rum, Tropical Drink Recipes, Hawaiian Cocktails, Hawaiian Drinks, Rum, Lanai, Molokai, Oahu, Maui, Kauai, Hawaii
About the Creator
Kohana Au has long been associated with a number of projects throughout Polynesia and the Hawaiian Islands. As a Featured Writer for the Kauai Menu Magazine he has extensive first hand experience with the islands. Kohana Au shares his passion and knowledge of the culture, history and legends of these very special societies and people. "As a world traveler, sailor and adventurer I have had the wonderful opportunity to experience and enjoy many places and people. I hope I can share with you, my friends and readers, just some of these adventures and places. " Aloha - Me ke aloha pau ole "with best wishes without end"
Log in or create an account to post a comment.
2 Comments
Kohana Au Mahalo nui loa Tonga! Enjoy!
Renee Rora Aloha Michael! Thank you so much for this! it is about time that people learned that Hawaii has the best rum in the world! The recipes are fabulous as well!


text:
If he makes it.
.... is to be sung by the 75-year old Englebert Humperdinck - younger forumites, ask your grandparents about him - whatdya' think, peoples ?
It might even work, he couldn't do much worse than most of the recent plonkers the U.K. has chosen, could he ?
..or could he ?
plain old vanilla
If he makes it.
O.M.G. I never thought we had sunk that low, that we had to get "The elderly" to enter this competition.Is this realy the best we can do?.
Guess if there is a WW3, they will get all those over 65 yrs old, and send them to the front line.
C'mon, G.B. we can do a damn site better than E.H. he is well past his sell by date, I think it was 40 yrs ago when he had his last hit.
Lincoln .7
There is no such thing as a problem, just a solution!!
Perhaps the most amazing thing about Engelbert Humperdinck is that it's his chosen stage name, his real name is Arnold George Dorsey! Why on earth would you choose Engelbert Humperdinck!!
Adler Tag ("Eagle Day") 13th August 1940
Why change his name from DORSEY, it didn't stop Tommy Dorsey from leaving his mark as a composer, entertainer, playing the trumpet and trombonist, and they didn't come much better than him.
Liincoln .7
There is no such thing as a problem, just a solution!!
Well there will be no hope for us if Sweden bring back ABBA!
Absolutely shamefull reply so that I could post this picture!
Lovely photo paul much better than EH LOL
rgds baz
[QUOTE=91Regal;1863836It might even work, he couldn't do much worse than most of the recent plonkers the U.K. has chosen, could he ?[/QUOTE]
Difficult to do much worse than last place.
The British team cannot seem to grasp the obvious:-
1/. Find someone who can sing.
2/. Find someone who looks good.
1 + 2 = Katherine Jenkins.
The foreigner's voting agenda has nothing to do with the quality of the songs or the acts these days. No point in rooting for Dinky, I'm afraid...
Never underestimate the power of stupid people in large groups!
the plain fact is !! we could front the best act in the world whilst an eastern european country could front a mute with a dog with a rough bark and if there were only the 2 acts we'd still only come second
the pure fact is that western european countries may as well no longer enter this political voting farce !!
in theory irrelavent of his age he should stand a fair chance , but i'm not holding my breath !!
my money would go on an ex communist country for a safe return !!
irony is the last time we won was with an american singer !!! , so even our last win technicaly wasn't european !!
Midlands Air Crash Research
"Lest We Forget"
Im not really a fan of Eurovision so could not give a rats bottom who we send to lose again. But sending EH as long as he is fit enough to sing is a pretty inspired choice. Look at the publicity its getting and the talking on the internet on forums just like this one. Its all free advertising for a Competition and a BBC show that a lot of people would not bother to watch otherwise. Anyone over 60 will watch for nostalgic reasons anyone under 60 will watch to see what somebody of 75 looks like singing, this is proper freak show stuff here
Realistically the UK will never win it due to political voting etc and I think this is why we do not really enter a decent established act each year, I think because we put our hands in to our pocket to fund the thing we can not be thrown out before the final so we are assured a place in the final even if we send David Cameron and Nick Clegg singing "I got you babe"
Also winning it means you need host it and obviously that costs so its rather better to enter and lose than enter and win, other than losing is a very British tradition we are great at it
To see my photos go to,
who actually decides our entry for eurovision??
Project Oblivion-Coming Soon
At The Going Down Of The Sun
And In The Morning
The best way for the UK to win is to have the following in our song:
- Cheesy pop song with simple English lyrics
- Some token phrases in German, French and one other language
- Praise for Britain integrating further into the EU failure and helping the other
money sucking b*stardsmember states
- Some mention about how great the other EU countries are, and how much we like them
Keep thy airspeed up, less the earth come from below and smite thee.
My Blog
My Designs
I think what Britons find frustrating is that in Europe, the UK is the source of most of the pop music from the last 50 years and yet cannot win a competition such as Eurovision. The reality is however that many countries vote politically and those that don't such as the UK, France, Germany etc. find themselves sidelined.
I think the UK should do as Italy did and withdraw from the competition.
Engelbert Humperdinck - is that truly the best we can do - well no, it isn't so I think it might an ironic choice - someone with a sense of humour. EH wasn't even a "great" in his heyday! What a bizarre choice.
Having said, we'll still watch it - car crash TV at its best.
So much for Pathos
When it was telephone voting Wogan used to be critical of countries voting for their friends but I think he somewhat missed the point; for example the large number of votes for Russia that were made in Estonia, a country occupied forcibly by Russia for about sixty years! What he failed to appreciate was that about 20% of the Estonian population is of Russian extraction so voting in the contest was one of the few ways this (persecuted?) minority population could get back at Estonia.
Ive often wondered how many of the UK votes for Ireland are from the Irish living in the UK? It has been said that the phone-boxes just north of the border are very busy on Eurovision night!
The only truly fair way for this competition to be voted on would be if nobody knew what nationality the singer is or country they are representing. But obviously that would make it a talent competion and not Eurovision.
My wifes friend from Belarus votes every year on whatever song she likes(she not biased) and says as she comes from a country where votes are rigged its one way she can use her vote and it really be counted.
To see my photos go to,
Can T22 WT525, Can B2 WD954, Pilatus P2 A-125 (cockpits)
"Wouldn't kick her out of bed.............mind you, with that song................I wouldn't wake her up, either..........."
well andrew loyd webber is highly regarded and when his song was entered we went from U.K. NIL POINTS , to being fith
but will EH break through the political vote
i think satan would be wearing ice skates !!
I just dont care .....I have Van Goughs ear for music !!
I think Nigel Farage and his rapping would go down pretty well
(Back ground info- Nigel is the leader of UKIP, and about the only politician making a stand again the unelected socialist EU bulldozer crushing the life out of Europe)
The song 'contest' is a bogus, rigged, corrupt and wasteful as the appalling Europe 'project' which spawned it. Perhaps if we ignore it, it will go away.
...having heard about Englebert, Czar Putin has decreed that the UK be upstaged....
...words almost fail me..
plain old vanilla
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|You are in: Health
Living in the shadow of a pyre
A Devon town is united in fear over the safety of chemicals released from an animal pyre.
Local protest forced the Ministry of Agriculture, Fisheries and Food (Maff) to scale down the number of carcasses being destroyed at a pyre near Holsworthy from 14,000 to 4,000.
Residents are frightened that the amount of cancer-causing dioxins filtering into the immediate vicinity will compromise their health.
But government guidance published on Tuesday states that dioxin levels should pose no long-term health threat, although it admits that people close to large pyres may suffer exposure to irritants.
One local doctor has already treated patients complaining of headaches and respiratory problems.
Although he cannot prove a direct association with smoke inhalation he believes there is a possible link, which could pose a long-term risk to health.
Dr Asad Al-Doori, who practises at Black Torrington, 11 miles from Holsworthy, said there were lots of "significant issues" to consider.
"Dioxins can lead to a serious suppression of the immune system and congenital deformities in children," he said.
"I hope the government and Maff have done their homework."
'Major concern'
The mayor of Holsworthy, John Allen, who runs a shop in the town, said everyone was worried about the health risks of the pyre.
He said: "I think it is one of the major concerns on everyone's lips.
"We don't know what the long-term effects are.
"People are concerned for themselves and their children."
Homes closest to the site of the pyre were evacuated and their owners temporarily re-housed by Torridge district council.
So far the wind has blown the smoke away from the town, but if it changes direction, existing fears will be intensified.
Mr Allen said: "People are worried about the fall out, what's in the dust and where it's settling."
The Department of Health guidelines state that people living close to pyres may suffer exposure to irritants such as sulphur dioxide, and asthmatics may find their condition worsens temporarily.
The government also admits that micro-organisms from carcasses could enter local water supplies, although public water companies are equipped to filter their supplies.
But the guidance states that dioxin levels around incineration sites will quickly return to normal and pose no long-term health threat, although levels in food and other samples taken from areas around larger pyres will be monitored.
Government scientists are currently monitoring dioxin levels near Holsworthy.
A DoH spokesman said: "When the results are available we will try to explain them to local people."
See also:
23 Apr 01 | Medical notes
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Original URL: http://www.theregister.co.uk/2006/04/12/buffalo_brin/
Buffalo to bring 'reincarnated floppy disk' to Japan
Follows Memorex, Verbatim
Posted in Personal Tech, 12th April 2006 10:55 GMT
Japan's Buffalo has become the latest storage product provider to offer M-Systems' 16MB FlashDisc, an apparently "exciting" new take on the old USB Flash disk concept that packages the solid-state storage into a circular shell reminiscent of 1970s computer tapes.
Buffalo this week joined Memorex in the US and Verbatim in Europe, the three "global brand partners" who are taking M-Systems' product to market. As are its fellows, Buffalo will be offering four of the 16MB, 6cm diameter disks on a spindle. It will make them available to Japanese buyers at the end of the month. ®


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Build a native android application
Бюджет $250-750 USD
Build android (java) application, im going to do this phase by phase to make sure the quality & commitment of the freelancer.
Phase 1:
1.1 splash screen (after install) - 2 pages
1.2. login page (authentication to server)
1.3 download list of data items (10 item initially, allow to download more items)
1.4 data available offline
1.5 sidebar menu
1.6 items page form
1.7 map page
1.8 web service to connect to backend database
backend app already done, in python & postgreSQL.
design already created (jpg/psd), you have to follow the design exactly.
pages to build in this phase:
1. info/splash
2. login
3. sidebar
4. homepage (item list)
5. item page/form
6. map page (multiple marker)
71 фрилансеров(-а) подали заявки на эту работу; средняя заявка - $627
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This is a revised edition of a work originally published in 1997. Vyse begins by discussing Wade Boggs, a baseball player who set some records that have never been equalled. His "professional life was filled with superstition". Believing that he hit better after eating chicken, he consumed it every day. He also had a long "pre-game ritual" (which I don't quite understand, not being familiar with the terminology of baseball). Vyse might similarly have mentioned Norman Parkinson, who in the 1970s was the official portrait photographer of the Royal Family. He would never pick up a camera unless he was wearing a fumi, an embroidered skullcap popular in parts of the Middle East. He believed that it would be bad luck to try to work without this headgear. It is easy to jeer at men like Boggs and Parkinson, but they did reach the summits of their respective professions.
Superstitions are more common with people whose life is uncertain or risky, for example "gamblers, sailors, soldiers, miners, financial investors, and, somewhat surprisingly, college students." I seem to recall that, decades after Donald Campbell drowned in Lake Coniston during an attempt to break the world waterspeed record, divers finally recovered his body, along with his lucky mascot, which evidently had not worked on that occasion.
As to students, whilst "conventional wisdom suggests that the highly educated should be more skeptical than their less learned peers", there is clearly a strong element of chance with exams: you may get a question on a topic you happened to revise that morning, or you may get a question on your weakest subject. Another uncertain profession is acting. Most actors are unemployed for much of the time, and when they do have work there are plenty of things that can go wrong.
For convenience, psychology professors frequently do tests on their own students. One wonders about this, since psychology undergraduates are probably not entirely representative of the human race as a whole, and knowing what is going on, may deliberately try to skew the results.
Vyse states that children's "rituals of avoidance of cracks in sidewalks . . . must have been passed from person to person". I cannot agree: I developed this habit at age five or six, and I am fairly sure that no-one had told me to do it, so it must be somehow instinctive. It was only later that I read A. A. Milne's poem 'Lines and Squares' (in When We Were Very Young, 1924), about a boy who believed that, if he "steps on a line" he would be eaten by bears. In my case I thought that a bomb would explode, although eventually I noticed that, when my thoughts were elsewhere, I had trodden on lines, but nothing untoward had ensued. Even in middle age I occasionally find myself treading carefully for this kind of reason.
A woman recently told me that, when a girl, she believed that, if she trod on a crack, the ground would open and the earth would swallow her up. If this kind of superstition were passed from person to person, one would expect that the anticipated disaster would always be the same. Nevertheless, superstitions are, as he says, likely to be acquired by membership of a social group, where, as has been shown by many psychological experiments, there is a tendency to go along with the others. (I would also query his assertion that dice rolling is "completely random". Whole books have been written about cheating at dice.)
Some birth horoscopes contain statements that must be true of almost anyone – "Some of your aspirations tend to be pretty unrealistic" – but people find them impressive. There are also some totally weird ideas, such as that "Dick Cheney was involved in the 9/11 attacks." In short, I would suggest that these notions are believed in because they are emotionally appealing, rather than because they are supported by facts and reasoning. – Gareth J. Medway.


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FSU Doctoral Candidate Discusses Alternative to Traditional Hospitals
COLLEGE PARK, MD – February 8, 2011 - Florida State University Economic Department Doctoral Candidate Nitin Dua will present his paper titled "Hospital Safety, Endogenous Entry and Competition by Ambulatory Surgery Centers" at Optimal Solution Group (Optimal) LLC's Brown Bag Lunchtime Series event at its office on February 9, at 12:00 p.m.
The paper will focus on the emerging trend of ambulatory surgery centers (ASCs) as a preferred setting for medical care as opposed to traditional hospital settings. Dua's research examines the potential for ASCs to provide easily accessible medical care and the merits of establishing more of these facilities in the future. He also argues that ASCs provide a safer setting for medical procedures as opposed to some hospitals known for having poor-safety ratings.
Dua's work stems from his research interests in Industrial Organization, Health Economics, and Labor Economics. He received his Master's degree in Business Economics from University of Delhi, India post which he worked as a quantitative researcher for a UK based multinational corporation.
Optimal Solutions Group, LLC
Founded in 2000, Optimal Solutions Group, LLC, is a nonpartisan public policy research and technical assistance firm that differentiates itself by its quantitative expertise and innovative approaches in order to provide rigorous, data-driven research and technical assistance to government agencies, corporations, nonprofit organizations, and philanthropic foundations. Located at the University of Maryland's M Square Research Park in College Park, Optimal is a leader in driving real-time public policy research. Optimal has 60+ employees including multidisciplinary researchers that have backgrounds in economics, health policy, statistics, psychology, sociology, public policy, communications, education, urban planning, and business. Optimal has four research centers—Health; Education; Housing; Workforce Development and Social Policy.


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Sherman Theater: Friday Night At The Fights - Frontline Assault
Sherman Theater: Friday Night At The Fights - Frontline Assault - Mixed Martial Arts (MMA) Event on October 5, 2012 in Stroudsburg, Pennsylvania USA
The Sherman Theater will begin to produce it's new MMA series "Friday Night At The Fights" and "Saturday Night At The Fights" with quarterly pro-am events. Frontline Assault is the first in the series of event. Featuring over a dozen bouts, the Sherman Theater production level will be comparable to being at a real arena. Real dressing rooms, real entrances, and great seating options will make this event a can't-miss experience.


text:
BioMed Research International
Volume 2013 (2013), Article ID 160320, 6 pages
Genetic Variation between Biomphalaria alexandrina Snails Susceptible and Resistant to Schistosoma mansoni Infection
1Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
2Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
Received 10 April 2013; Revised 13 June 2013; Accepted 15 June 2013
Academic Editor: Robert Stoehr
Copyright © 2013 Suzanne M. F. El-Nassery et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction between Biomphalaria alexandrina snails and Schistosoma mansoni. Using RAPD-PCR with eight (10 mers) random primers, we were able to determine the polymorphic markers that differed between snails susceptible and resistant to Schistosoma mansoni infection using five primers out of the eight. Our results suggest that the RAPD-PCR technique is an efficient means by which to compare genomes and to detect genetic variations between schistosomiasis intermediate hosts. The RAPD technique with the above-noted primers can identify genomic markers that are specifically related to the Biomphalaria alexandrina/Schistosoma mansoni relationship in the absence of specific nucleotide sequence information. This approach could be used in epidemiologic surveys to investigate genetic diversity among Biomphalaria alexandrina snails. The ability to determine resistant markers in Biomphalaria alexandrina snails could potentially lead to further studies that use refractory snails as agents to control the spread of schistosomiasis.
1. Introduction
Schistosomiasis is the second most common parasitic cause of mortality in tropical countries, second only to malaria, and has been a target for increased control by the World Health Organization [1]. Great effort has been made to control the transmission of the disease. However, there is little evidence that the prevalence of the disease has decreased globally. Indeed, it continues to spread to new geographic areas [2]. Of the countries in which neglected tropical diseases are prevalent, Egypt is one of the many counties that have suffered greatly under the burden of tropical diseases, including schistosomiasis [3]. Schistosoma infection is one of the most significant public health problems facing the country.
Human infection with Schistosoma mansoni is closely related to the existence of its intermediate snail host of the genus Biomphalaria. Biomphalaria alexandrina is the only snail host in Egypt [4, 5]. This snail has extended its distribution from the Nile Delta and is now present throughout the country along the tributaries of the Nile. By 1979, B. alexandrina had colonized the Nile from the Delta to Lake Nasser [6]. Recently, B. alexandrina snails have invaded the water sources in some reclaimed areas, which has resulted in Schistosoma infection in previously uninfected populations and will eventually lead to an increase in schistosomiasis transmission throughout Egypt [7]. The situation may be further complicated by the increasing number of irrigation projects whose goal is to improve the Egyptian economy along with the increasing mobility of infected agricultural workers who migrate to neighboring areas [8, 9]. Schistosomiasis is a specific target for control by the Egyptian Ministry of Health, international agencies, and by the World Bank. This has been done via the mass administration of the medication praziquantel to human populations in conjunction with the use of niclosamide for snail control. However, schistosomiasis control has not yet been achieved [10]. With the appearance of praziquantel resistance among schistosomes [11] and the absence of an available vaccine for use [12], the use of alternative control strategies is necessary. The high degree of specificity of schistosomes for their intermediate snail host led Coelho et al. [13] to suggest a control strategy based on the use of snails resistant to parasitic infection as biological competitors of existing susceptible snails in endemic areas. However, the application of this strategy requires an in-depth understanding of the role of genetics in the interaction between parasites and snails.
Newton [14], a pioneer in the study of the genetics of Biomphalaria glabrata, considered the susceptibility or resistance of a snail to infection to be a heritable characteristic. Resistance is found to be a dominant character in many Biomphalaria spp. However, some loci of susceptible snails could carry a certain amount of resistance alleles [15, 16]. The increase in the number of inherited resistant genes could lead to an increased immune response to the parasite and decrease the degree of susceptibility to infection [16–18].
Resistance in Biomphalaria snails has been found to be age dependent. In B. glabrata, the resistant phenotype in juvenile snails is controlled by four genes, each of which has multiple alleles, while in adulthood, there is only a single dominant gene that determines this trait [19, 20]. In B. tenagophila, two dominant genes determine resistance [17]. The susceptibility and resistance of B. alexandrina, as with other Biomphalaria spp., were found to be heritable characteristics, with the resistance trait being dominant [16, 21]. However, the number of genes that determine this characteristic has not yet been identified.
One of the factors limiting progress in understanding the molecular genetics of B. alexandrina relative to other Biomphalaria species has been the lack of specific sequence information for the application of polymerase chain reaction (PCR). An alternative powerful and highly applicable technique that does not require prior sequence information is the random-amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Unlike conventional PCR-based analyses, this approach uses oligonucleotide primers of an arbitrary sequence to start DNA strand synthesis under conditions of low stringency. Each primer allows for the amplification of specific fragments that are randomly distributed throughout the genome of the snail, thus allowing for unbiased comparison [22, 23]. Many investigators have used this technique to distinguish between susceptible and resistant phenotypes in different Biomphalaria species due to its high applicability [24–27]. In this study, we sought to determine the genetic variability related to the susceptibility and resistance of B. alexandrina snails to S. mansoni using the RAPD-PCR technique with different primers.
2. Materials and Methods
2.1. Snails Stocks
All snails originated from water channels in the Alexandria governorate (Egypt). They were maintained for one year in our laboratory under suitable environmental conditions in glass aquaria containing snail-conditioned water, and they were fed lettuce leaves and calcium carbonate. The parasite strain was originally obtained from shedding cercariae from naturally infected B. alexandrina snails collected from Alexandria water courses. Maintenance of S. mansoni life cycles was conducted between the snails and Swiss strain albino mice. Separation of Schistosoma resistant and susceptible snail stocks was performed according to the method described by Zanotti-Magalhães et al. [28], which is as follows: 100 juvenile snails (four to six mm in diameter) were used. Each snail was exposed for four hours to eight freshly hatched miracidia of S. mansoni. Susceptibility was monitored weekly from four up to 10 weeks postexposure through cercariae shedding which was favored by lamp light. Nonshedding snails were reexposed individually to eight miracidia/snails. Any snails in which the cercareia were observed were considered infected, and those that did not shed cercariae after two exposures were considered uninfected. The infection rate was calculated.
Snails that remained uninfected after two exposures were isolated and reared singly for self-reproduction under laboratory conditions. Their progeny was the resistant stock. Snails that yielded high infection rates were isolated and reared singly for self-reproduction, and their progeny was the susceptible stock. Thirty days post infection, six snails from each stock were used in the present study.
2.2. Molecular Analysis
2.2.1. DNA Extraction
Snail tissue (both susceptible and resistant) was dissected, and the head-foot of each snail was preserved in absolute ethanol for the extraction of high molecular weight DNA [4, 29]. EZ-10 spin column genomic DNA extraction kit for animal tissue (Bio Basic Inc., Canada) was used. Thirty mg of the snail tissue was placed in a 1.5 mL centrifuge tube, and 300 μL of Animal Cell Lysis Solution and 20 μL proteinase K were added. The mixture was incubated at 55°C with occasional vortexing until the tissue was completely lysed (at least three hours) and cooled to room temperature. The tube was mixed by vortex for 20 seconds and then centrifuged at 14500 ×g for five minutes. 300 μL of the supernatant was then pipetted into an EZ-10 spin column, and 300 μL of AB buffer solution was added. The preparation was mixed well and allowed to sit for two minutes. Centrifugation was completed at 1600 ×g for two minutes, and the flow through was discarded. 500 μL of the wash solution was added, and spinning was conducted at 6400 ×g for one minute. Washing was repeated, and the flow-through was discarded. Spinning was completed at 10060 ×g for an additional minute to remove the residual amount of the wash solution. The column was placed into a clean 1.5 mL microfuge tube, and 45 μL elution buffer (2.0 mM Tris-HCl pH 8.0–8.5) was added to the central portion of the membrane in the column. The tube was incubated at 50°C for two minutes. Spinning was conducted at 10060 ×g for one minute to elute DNA from the column.
The quantity of DNA was measured spectrophotometrically by UV absorption at A260 (1.0 OD unit is equivalent of 50 μg). The quality of genomic DNA was assessed via an analytical 0.7% agarose gel.
2.2.2. DNA Amplification by RAPD-PCR
The genotypes of the resistant and susceptible strains of B. alexandrina snails were determined with different arbitrary 10 mer primers using the RAPD-PCR technique according to a method described by Simpson et al. [30] and Abdel-Hamid et al. [25] with some modifications. The technique was performed in a reaction volume of 25 μL using 25 ng genomic DNA of each sample, 50 pmoL of each primer, 10X Taq DNA polymerase buffer including MgCl2, 0.2 mM dNTPs, and five unit/μL Taq DNA polymerase (Promega Co., USA).
Eight 10 mer primers were tested for their ability to differentiate between susceptible and resistant strains of B. alexandrina to S. mansoni. The primers used were as follows: OPA-01 (5′-CAGGCCCTTC-3′), OPA-02 (5′-TGCCGAGCTG-3′), OPA-06 (5′-GGTCCCTGAC-3′), OPB-18 (5′-CCACAGCAGT-3′), OPC-11 (5′-AAAGCTGCGG-3′), OPD-10 (5′-GGTCTACACC-3′), OPD-18 (5′-GAGAGCCAAC-3′), and OPM-04 (5′-GGCGGTTGTC-3′) (Operon Technologies Inc. CA, USA). The primers OPA-01, OPA-02, OPA-06, and OPM-04 have been used in previous studies in which specific polymorphic markers were detected between resistant and susceptible strains of different snail species to S. mansoni infection. They were used in B. glabrata [24, 26, 31, 32], B. tenagophila [25], in both B. glabrata and B. tenagophila [33], and B. alexandrina [21]. To our knowledge, the remaining random primers had not yet been tried in Biomphalaria spp.
The lyophilized primers were purchased from Eurofins (mwg/operon). They were reconstituted by adding sterile water to a final concentration of 100 pmoL/μL distributed in aliquots and stored at −20°C. The amplification conditions were as follows [21]: the tubes were transferred to the thermal cycler where they were subjected to one cycle of initial denaturation at 95°C for five minutes, then 40 cycles of 95°C for one minute, 30°C for one minute, 72°C for one minute followed by a final extension cycle at 72°C for 10 minutes.
2.2.3. Agarose Gel Electrophoresis and Staining
The PCR products were separated on 2% agarose gel stained with ethidium bromide, visualized on a UV Transilluminator, and photographed by Gel Documentation System (Alpha Imager M1220, Documentation and Analysis System, Canada).
2.2.4. Analysis of Polymorphisms on Agarose Gels
The genetic variability of the susceptible and resistant strains was determined by analysis of the electrophoretic profiles of the bands visualized on the gels. The identification of polymorphic bands was based on the comparison of the band patterns on the same gel for the susceptible and the resistant strains, and only those detected in all individuals of the same strain were considered polymorphic [24, 33]. The similarity coefficient was calculated as described by Dice [34] (Table 1).
3. Results
The susceptibility or resistance of B. alexandrina snails to S. mansoni infection was studied starting four weeks postinfection and continuing weekly thereafter for up to 10 weeks after miracidia exposure. The obtained results showed that 68% of the examined snails (100 snails) were susceptible, while 32% of the snails were resistant.
In this study, the susceptible and resistant snail lines were selected on the basis of well-characterized resistance/susceptibility phenotypes after uninfected snails were exposed to S. mansoni miracidia twice. The RAPD-PCR method was used to differentiate between susceptible and resistant snail lines of B. alexandrina. A total of eight primers were tested, and polymorphic markers were obtained with five of them (OPA-02, OPB-18, OPC-11, OPD-10, and OPD-18). We found that with these primers, the method was reliable for the identification of stable genomic markers between the two phenotypes, that is, the susceptible and resistant traits within the same snail species. The other three primers, OPA-01, OPA-06 and OPM-04, did not produce any results.
The genomic DNA amplified by using the primer OPA-02 (5′-TGCCGAGCTG-3′) gave one polymorphic band of approximately 730 bp in the susceptible strains only and three differentiating bands of nearly 330, 430, and 670 bp in resistant strains. As shown in Figure 1, the genomic DNA amplified by using the primer OPB-18 (5′-CCACAGCAGT-3′) presented a polymorphic band being approximately 700 bp in the susceptible strains only. In resistant strains, the primer yielded three differentiating bands of nearly 230, 260, and 470 bp. The results obtained from the electrophoresis on agarose gel of the amplified products of genomic DNA with the primer OPC-11 (5′-AAAGCTGCGG-3′) showed a number of differentiating bands in both susceptible and resistant strains. The genomic DNA, which was amplified with this primer, presented with a specific band of 400 bp in susceptible lineages and four bands of approximately 290, 380, 540, and 1010 bp in the resistant lineages (Figure 2). One band only of 400 bp in the susceptible linage and another one of 260 bp in the resistant linage were obtained using the OPD-10 (5′-GGTCTACACC-3′) primer. The amplified genomic DNA with the primer OPD-18 (5′-GAGAGCCAAC-3′) presented with two differentiating bands of nearly 230 and 1000 bp in the susceptible lineages and three specific bands of 150, 300, and 500 bp in resistant lineages.
4. Discussion
The infection rate obtained in the present study demonstrates that B. alexandrina is an efficient intermediate host for S. mansoni and consequently plays an important role in schistosomiasis transmission in Egypt.
The RAPD-PCR technique was used in the current work to determine the susceptibility/resistant genetic variability of B. alexandrina snails. Eight ten base pair (bp) oligonucleotide primers were each added to an individual sample of DNA, which was then subjected to PCR. The resulting amplified DNA bands were polymorphic segments with different band sizes depending upon the genomic DNA and the primer. The RAPD-PCR products were analyzed using agarose gel, which represents an effective and cheap method [33, 35]. However, other authors have used the alternative polyacrylamide gel electrophoresis method [21, 33, 36].
The results of the current work reveal the low coefficient of similarity between the susceptible and resistant snails studied (Table 1). This may be due to the short period (one year) allotted for snail rearing in the laboratory. In a study by Spada et al. [26] in which snails were reared for 20 years in the laboratory, the authors yielded a high coefficient of similarity. These authors expected low genetic variability of their strains and concluded that laboratory strains undergo much less intense selective pressures than the field isolates have.
Of the eight primers used in the present study, three primers, namely, OPA-01, OPA-06, and OPM-04, whose sequence of oligonucleotides are (5′-CAGGCCCTTC-3′), (5′-GGTCCCTGAC-3′) and (5′-GGCGGTTGTC-3′), respectively, were unable to detect polymorphic markers between the resistant and susceptible lines of B. alexandrina snails. However, these primers were efficient in distinguishing genetic variability of the resistant and susceptible lines of B. glabrata and B. tenagophila [24, 26, 32, 33]. These primers might amplify very restricted areas of the genomes of B. glabrata and B. tenagophila specific for resistance or susceptibility, which are not present in B. alexandrina snails. Interestingly, one of these primers, the OPA-01 primer, resulted in high genetic variability between B. alexandrina strains from different provinces in Egypt using the RAPD-PCR technique [37]. The present study showed that with five out of the eight utilized primers, specific bands of different lengths were amplified in the resistant and susceptible phenotype snails. To our knowledge, four of the five utilized primers (OPB-18, OPC-11, OPD-10, and OPD-18) had not yet been studied with Biomphalaria spp. The fifth primer OPA-02 (5′-TGCCGAGCTG-3′) had been tried previously with different Biomphalaria species [21, 25, 26]. In the current study, this primer resulted in three polymorphic bands with nearly 330, 430, and 680 bp in the resistant strains and one band with 730 bp in the susceptible strain. Abdel-Hamid et al. [21] used the same primer on B. alexandrina originating from the Delta region of Egypt to determine markers of resistance. They obtained only one band with 430 bp in the resistant strains, which we also obtained in the current study. The OPA-02 primer was also used by Abdel-Hamid et al. [25] and Spada et al. [26] for the analysis of the genetic variability between resistant and susceptible lineages of B. tenagophila and B. glabrata. Using this primer, the authors were unable to detect any polymorphic markers between resistant and susceptible lines. This marker may be a specific marker distinguishing the resistant and susceptible B. alexandrina from other species.
In the current work, we searched for primers capable of differentiating susceptible and resistant strains. As mentioned above, five of the eight utilized primers were able to amplify specific bands of different lengths between the resistant and susceptible phenotype snails. In addition to the OPA-02 primer, which was used in previous studies, we tried four random primers that, to our knowledge, have not been used before with Biomphalaria species. These primers are OPB-18 (5′-CCACAGCAGT-3′), OPC-11 (5′-AAAGCTGCGG-3′), OPD-10 (5′-GGTCTACACC-3′), and OPD-18 (5′-GAGAGCCAAC-3′). Each of these primers was able to demonstrate one or more differentiating polymorphic bands in the resistant and susceptible strains in B. alexandrina.
We found that the detection of susceptible and resistant bands using the RAPD-PCR technique is a suitable and efficient methodological approach for the analysis of genetic variability among schistosomiasis intermediate hosts. However, future studies should sequence the PCR products to predict the function of the identified DNA sequences and to assist in explaining the levels of polymorphism reported between the susceptible and resistant snails.
As has been concluded by other researchers, the RAPD-PCR technique was found to be sensitive, fast, and easy to perform [32, 38]. Using the RAPD technique with the abovementioned primers can identify genomic markers that are specifically related to the B. alexandrina/S. mansoni relationship in the absence of specific nucleotide sequence information. Consequently, this approach can be used in epidemiologic surveys investigating the genetic diversity of B. alexandrina snails, as it is a time-saving technique when compared to the conventional parasitological methods. The ability to determine resistant markers in B. alexandrina snails could pave the way for further studies based on the use of refractory snails as a means by which to biologically control the transmission of disease, although this issue remains a matter of discussion. However, the use of this approach in concert with other measures could create new strategies for the control of schistosomiasis transmission.
Conflict of Interests
The current authors paid all the expenses of the study, with no financial support from the university or from any other institutions. There are no personal relationships with other people or organizations that could inappropriately influence (bias) the work. No one of the authors of this paper have any direct financial relation with the commercial identities mentioned in the paper that might lead to any conflict of interests for any of the authors.
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She became silent at that, and for a time the low sweet harmonies of the nocturne Penelope was playing filled the gap.
In fact it is so popular that when any one is asked to play "Chopin's nocturne," this one is meant.
The (p. 305) 'nocturne in black and gold,' I do not think a serious work of art.
The yacht in nocturne is the yacht I want, ought to have, and never shall have.
He had a supper by appointment, but began to write his nocturne and forgot all about the time.
Before the end of the nocturne carriage bells are heard outside.
I could not appreciate their mental pleasures, any more than a savage could delight in a nocturne of Chopin.
A nocturne—yes; it was getting dark, and the sea was rising—that was the sound of the sea.
I shall, without your permission, and probably to your disgust, play a nocturne by John Field.
Lady Constance sat down at the piano and began the nocturne.
1862, "composition of a dreamy character," from French nocturne, literally "composition appropriate to the night," noun use of Old French nocturne "nocturnal," from Latin nocturnus (see nocturnal). Said to have been coined c.1814 by John Field, who wrote many of them, in a style that Chopin mastered in his own works, which popularized the term.


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අනාගතයේ දී, සෑම දරුවෙකුම නව නිපැයුම්කරුවෙකු වනු ඇත
We are enabling children around the world to get creative with technology and invent in school, in clubs and at home! The BBC micro:bit is a fun, handheld, easily programmable computer that was rolled-out across the UK in 2016 - and is now being used around the world. It's 70 times smaller and 18 times faster than the original BBC Micro computers used in schools in the early 1980s. Started by the BBC and a great team of partners, the Micro:bit Education Foundation is a non-profit organisation.
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The micro:bit was created using the ARM mbed hardware and software development kits (HDK and SDK) and compiler services. The project builds on the organisations' collaboration on the original 1981 BBC Microcomputer.
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The micro:bit was originally envisioned and created by the BBC together with the collaboration of over 30 partners, inspired by the BBC micro. BBC Learning will continue to produce content and projects that support the Foundation's U.K. Education strategy.
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To bring the micro:bit to life, Microsoft have provided a great development experience with Block Editor and TouchDevelop. They have also brought in a number of learning resource providers, and are producing a getting started guide for teachers and students.
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Samsung developed the official micro:bit app for Android that connects the micro:bit to smartphones and tablets, enabling children to code the micro:bit on-the-go. The unique set of features on the app allows young people to learn how to code to control their devices via the micro:bit, creating endless applications for young people to develop useful and fun technology solutions. For example, they can learn how to build their very own micro:bit selfie remote controller! Samsung has developed a range of free downloadable resources and projects for schools and families.


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Hello, Welcome to Just Answer and thanks for your question. Aspartamine taken in reasonable quantities has never been found to present any harm to anyone in scientific studies. Asthma itself is quite serious/dangerous and treating it is imperative. Singulair is a leukotriene inhibitor that prevents allergens from causing swelling in the lungs and airways. It is used in children as young as 2, often in conjunction with nebulizers. The puffiness in the face may be a result of the pulmicort which is a corticosteroid that may result in redistribution of body fat in different parts of the body, including the face. You should discuss your concerns with his treating doctors.


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One is for Ben Day, a young man who was killed in action in the waning days of World War I. He's the young man whose body was brought back to Hopkins County via train and spent the night in the Hopkins County Courthouse before being taken by wagon to its final resting place.
I'm also always drawn to the gravesite of Ervin Crabtree, who was killed in action in World War II. Ervin, newly married to a young lady from Reilly Springs, was a "wet-behind-the-ears farmboy" who went to serve his country like so many others and never made it back home. He gave the ultimate sacrifice for his country's freedom.
I visited Tuesday with Vera Harrington, who reported enjoying the beautiful weather. She indicated that she'd had time to get out and do some of the things she needed doing and enjoy the cool temperatures and warm sunshine.
Vera had a nice visit with Mary and the girls, as they came for the weekend and spent time on the farm in Reilly Springs. "I really hated to see them go back," said Vera. "We'd all had so much fun, laughing and enjoying all the things here in Reilly Springs, but work and school meant they had to return to Waxahachie."
Congratulations are in order for Trey Hinton, who won Grand Champion honors in the Hopkins County Hay Show. He and Wayne IV were on hand to accept the honors at the Professional Ag Workers Breakfast last Thursday.
Marilee Fishere expressed her sorrow at the loss of her high school friend, Lynn McKinney Reynolds. Marilee, Chad Cable and Nita Gouge Brewer all attended the funeral last Saturday.
Then on Sunday, Marilee, Ethel Winton and Ima Asher attended a birthday party for Mrs. Wilma Brice and had an enjoyable time visiting with friends and wishing Wilma a happy birthday.
The Fisher family is busy processing deer and hogs. They're making summer sausage and cutting meat and are available "around the clock." J.R., Vanessa, Blake and Kendra are all involved, along with Fred Fisher and his grandson Drew Cross. So if you kill that big buck, you know where to take it.
Joyce McDonald and Ruth Fisher are making plans for a trip to Branson, Mo., so Fred may have to eat some of that sausage while they're gone.
Residents in Reilly Springs awoke Saturday to everything being covered in frost. It was a beautiful morning, but just a little bit cool, and that about stopped the yard mowing and the cutting of hay in our community for the season.
Poeple in Reilly Springs, along with others in Hopkins County, were saddened to learn of the death of Mr. Malcolm Wright, who passed away on Sunday. Malcolm was a frequent visitor to this community and made a remarkable difference in this life. He will be sorely missed by his many friends and family members.
Our own Karman Holland was one of the presenters in this year's annual Extension Service Holiday Program, held Monday afternoon and evening in the Southwest Dairy Museum to usher in the holidays. She demonstrated a number of ways to use natural "gatherings" from your yard, the woods, and the countryside in decorating your home for the holidays. The Christmas program was well attended with a number of crafts, recipes and gift-giving ideas for the holidays.
Until next week, continue to remember our troops, who defend our freedoms and ask for them an extra measure of safety and safe trip home. Pray for love in our hearts, harmony in our community, and peace in our land. God bless Reilly Springs, Hopkins County, and America.
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breath palette toothpaste
Finally more than just bubblegum flavored toothpaste... this has chocolate, caramel, orange...
Turn the boring morning and nightly routine into a party. That's right! A party in your mouth with Cupcake Floss! Be careful though, the delicious frosting flavor might turn you into a flossaholic!
I have an obsession with bacon, so my boyfriend bought me this as a joke. Pretty darn fanstastic if you ask me!Added 9 Years Ago from wishingfish