text:
This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Marine systems are very diverse and recognized as being sources of a wide range of biomolecules. This review provides an overview of metabolite profiling based on mass spectrometry (MS) approaches in marine organisms and their environments, focusing on recent advances in the field. We also point out some of the technical challenges that need to be overcome in order to increase applications of metabolomics in marine systems, including extraction of chemical compounds from different matrices and data management. Metabolites being important links between genotype and phenotype, we describe added value provided by integration of data from metabolite profiling with other layers of omics, as well as their importance for the development of systems biology approaches in marine systems to study several biological processes, and to analyze interactions between organisms within communities. The growing importance of MS-based metabolomics in chemical ecology studies in marine ecosystems is also illustrated.
Automated mass spectral deconvolution and identification system
Aerosol mass spectrometry
Atmospheric pressure chemical ionization
arachidonic acid
Culture collection of algae and protozoa
Diode array detector
Desorption electrospray ionization
Docosahexaenoic acid
Electron capture negative ionization
Evaporative light scattering detector
Electronic impact
Gas chromatography
Integrated Infrastructure Initiative
Inductively coupled plasma
Framework Programme 7
Fourier transform ion cyclotron resonance
Laser ablation electrospray ionization
Liquid chromatography
Matrix assisted laser desorption ionization
Multiple reaction monitoring
Mass spectrometry
Next Generation Sequencing
Negative ion chemical ionization
Nuclear magnetic resonance
Polyunsaturated aldehydes
Polyunsatured fatty acids
Stir bar sorptive extraction
Secondary ion mass spectrometry
Solid phase extraction
Solid phase microextraction
Trimethylsilyl derivatives
Time of flight
Ultra performance liquid chromatography
Volatile halogenated organic compounds
Volatile organic compounds.
Seas and oceans cover more than 70% of the surface of the globe. Marine environments are rather diverse, some of them being either stable or unstable, and this heterogeneity is related, to some extent, to distinct physico-chemical conditions like salinity, UV radiation, temperature, and pressure. Such habitats are occupied by a great diversity of prokaryotic and eukaryotic organisms, autotrophic or heterotrophic, accounting for about 2.2 million species of microbes, algae, plants, and animals according to recent estimations [
One feature supporting this biodiversity is contained inside each individual, in the genetic information. Marine organisms possess an extraordinary variety of—often unique—structures related to different biological processes such as metabolic pathways, reproductive systems, development patterns, and sensory and defense mechanisms. Over the last decade, marine biologists have been extremely active in applying genomics at both the organism and the ecosystem level, and the quantity of genomic resources on marine organisms is now becoming highly significant. These resources can be found in several databases with different focus, such as Megx, CAMERA JGI, and GOLD (
There is a long tradition to explore marine diversity in order to discover new molecules and identify bioactive compounds, since marine organisms produce a wide array of chemicals [
Measurement of metabolites is performed by metabolomics, a rather new aspect in the field of omics techniques, in particular when compared with approaches used to sequence genomes and to profile RNA and proteins. Nuclear magnetic resonance (NMR) and mass spectrometry (MS)-based techniques are among the most popular technologies available to perform medium to high throughput metabolite profiling, and have already been used extensively, for instance for phenotyping of several benchmark biological models, or for investigating interactions of living organisms with their environment (environmental metabolomics) [
Database links.
|HMDB (Human Metabolome Database)
|GMD (Golm Metabolome Database)
|FiehnLib (Fiehn Metabolome library)
|SWMD (Seaweed Metabolite Database)
|Data Processing Links
|Metabolome Express
The introduction of state-of-the-art genomic approaches to marine biology has stimulated ground-breaking technological and theoretical advances, leading to the development of major new avenues of research. Recent technical developments in the field of metabolomics allow forecasting comprehensive metabolomics analyses in marine systems [
In this review, we attempt to illustrate the diversity of analyses conducted so far by targeted and untargeted MS-based profiling approaches in marine (eco)systems. To achieve this aim, we focus on recently published advances in the field.
Bacteria have so far been the most extensively studied marine organisms for metabolite profiling. Use of 13C labeling techniques with GC-MS analysis [
Fractions of the metabolite content of other bacteria have been reported by targeted analysis. Phenazines, which are of special interest due to their antimicrobial activities, were investigated in bacteria affiliated to
Among the Bacteroidetes,
Alongside heterotrophic bacteria, cyanobacteria are key players in aquatic environments. Most of the metabolomic MS-based studies for this type of bacteria focus on
For many years, chemical profiling of marine algal extracts was restricted to targeted identification and quantification of selected classes of compounds relevant in the context of economical uses and/or of ecophysiology of these organisms. Aqueous extracts were particularly investigated to measure carbon metabolism-derived compounds such as sugar-nucleotides, polyols, storage and cell wall polysaccharides, using GC-flame ionization detector (FID), GC-MS, HPLC, or NMR methods. Other GC-based studies measured sterol compounds and free fatty acids that were further characterized when MS became more accessible for algal research. Pigment profiling was also very extensively used to describe the different taxa of algae, with major qualitative differences observed between the main algal lineages (red algae, green algae, Rhizaria, Dinoflagellates, Stramenopiles). These organic extracts are indeed very complex mixtures and have imposed major challenges for the analysts.
There are only a limited number of recent metabolomics analyses of microalgae that have been conducted using MS-based approaches, and most of them concern diatoms, this is why we focused on these organisms in the following section.
Most of these data have been produced by GC-MS technology, due to its high sensitivity and robustness for targeted metabolites such as fatty acids, amino acids, oxylipins, or natural products. In 2009, Nappo
In addition to these rather broad analyses, more targeted studies have brought a lot of information on diatom metabolism. For instance, Lang
To finish, it is noteworthy to mention that volatile halogenated compounds have also been detected in diatoms. Recently, cyanogen bromide (BrCN) has been demonstrated to control biofilm formation [
In 2011, the first high coverage metabolomics study on red algae was published [
Indeed, the wound response of the red alga
Metabolite profiling was not restricted to lipid metabolism in red algae, but applied also to several classes of secondary metabolites. Quantification of mycosporine-like amino acids (MAAs), compounds involved in stress responses in several types of marine photosynthetic organisms, has been performed in
Halogenated compounds are well known in red and brown macroalgae, and their metabolism continues to attract interest, as illustrated by the recent publication of a comparative study of volatile halogenated compounds emitted by seaweeds [
Brown algae are also considered as reservoirs for other types of secondary metabolites. For instance, Kledjus
During the last decade, several methods to identify potential chemical markers of defense responses in brown macroalgae have been developed. For instance, hydrodistillation, focused microwave-assisted hydrodistillation, or supercritical fluid extraction, followed by GC/MS analysis, have been applied for extraction of volatile metabolites from
Most of the studies dealing with marine green macroalgae have so far focused on lipid metabolism. Variability of the fatty acid content within the genus
To close this section on macroalgae, it is interesting to note that there are a number of comparative analyses that have been performed to assess different metabolic abilities in seaweeds, in particular production of fatty acids, as illustrated by at least three publications in 2011. A first one analyzed the fatty acid composition of nine seaweed species from the North Sea and from tropical seas in the context of seaweeds being a good, durable, and virtually inexhaustible source of polyunsaturated fatty acids (PUFAs) [
Metabolomic data on marine animals have mostly been acquired by NMR [
Targeted metabolomic studies have been conducted on other animals such as harbour seals and harbour porpoises [
Several types of mollusks have been considered recently for MS-metabolite profiling. One of the examples is the investigation of tidal cycle effects on mussel (
Sponges are recognized as interesting sources of natural products with therapeutic applications, and many of the marine species host
Corals, sessile animals among which most of them rely on photosynthetic microalgae (Zooxanthellae) to obtain the majority of their energy and nutrients, are a well-studied family of marine organisms because of their high content in natural products of pharmaceutical interest [
It has been known for a long time that different types of living organisms secrete a large number of metabolites into their environment (especially under conditions of unbalanced growth) [
An UPLC-MS approach, designed by Barofski
In macroalgae, a number of studies, conducted with ecologically relevant methodologies, have focused on secondary metabolites involved in the defense against biofoulers and pathogens [
In spite of these fascinating progresses in the understanding of novel chemical communications within communities of marine organisms, no global metabolomics study on environmental seawater samples has been published yet. So far, most results of targeted profiling have been obtained on supernatant of controlled cultures. It remains to be demonstrated that dilution effects occurring in field conditions do not preclude the detection of cues that are relevant in the context of shaping population and community structures. This work needs to use approaches without
Developments of
Salts, mainly NaCl, represent major interfering compounds. Moreover, salts polymerize in the ion source and create adducts which increase the difficulty of identifying molecular ions [
Another challenge for metabolite extraction, in particular for macroalgal samples, is grinding. Indeed, seaweeds contain a lot of polysaccharides. For instance, in red algae, carrageenans confer a gelling nature to tissue, resulting in difficulties to obtain a reproducible manual grinding. Thus, particular attention has to be paid to grinding, and automated techniques to carry out such extraction step, e.g., cryo-grinding, should be preferred. To avoid metabolite degradation, freeze-drying of sample before grinding is an option; however, no study has been published yet on the comparison of metabolomic profiles of marine organisms obtained either by freeze-drying samples before grinding or by directly grinding samples in liquid nitrogen. Therefore, it is difficult to give any specific recommendations.
Other types of molecules, such as pigments, represent a major part of algal metabolite content [
This type of experiment represents a great challenge, and we describe here different methods to extract chemicals from the seawater matrix.
Liquid/liquid extraction is one of the most current techniques to work on liquid samples. For instance, marine antifouling compounds organotins were extracted using liquid/liquid extraction, after derivatization, and then detected by either GC-MS or LC-APCI-MS (liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry) [
Substitutes to SPE exist, based on passive samplers such as polymethyldimethylsiloxane (PDMS). To quantify organotin in seawater samples, Chou
Another challenge to conduct metabolomic studies is data treatment. For MS data, several pipelines are available such as XCMS [
Metabolomic data obtained for two strains of
Identifying metabolites is a bottleneck in the metabolomic field. Wishard recently reported the complexity of identifying compounds (1000 to 200,000 different chemicals) when compared to the identification of genes and proteins [
The KEGG and "Cyc" databases are examples of some of the most popular metabolic pathway databases. There is, therefore, a need to develop specific "Cyc" databases dedicated to some of the marine models for which genomes are sequenced (
Finally, it is also possible to suggest the building of organism-specific comprehensive metabolomic databases to combine metabolic information gained for specific marine organisms, and to link this type of repository to the other kinds of web resources listed above, to aim at knowledgebase(s) similar to the Human Metabolome Database (HMDB) [
Soon, the community of marine chemists, biologists, and ecologists will have the capacity to rapidly identify a large number of metabolites from various marine organisms or seawater samples using hyphenation of GC-MS and LC-MS. It will also be possible to identify metabolites without ambiguity using MS-MS. Details for the experimental procedures and analyses of data will be important to be recorded in the framework of the minimal requirements for publishing metabolite data [
Quality control and standardization of metabolomics data are important technological points that are well considered in the biomedical and plant research fields, and are necessary to guarantee meaningful biological applications. Therefore, it is essential, in the nascent field of marine metabolomics, to anticipate these issues by increasing integration of platforms featuring different levels of specialization in some extraction procedures and MS-based approaches (
MetaboMER workflow for a metabolomic approach of marine ecology (adapted from [
Integration of data and systems biology approaches have already been applied to different marine systems, without any metabolite profiling data. Thus, different sorts of models are available for marine organisms, in particular for some selected organisms within their corresponding phylogenetic lineage. To study early development of the brown algal model
Adding analysis at the level of metabolomes with other omics datasets (such as transcriptomes and proteomes to cite the most common) should help to go deeper in the understanding of the processes going on at the scale of an organism (cell, tissue, whole organism), and of the relationships within communities (population scale). Use of organisms considered as biological models within each of the phylogenetic lineages found in the seascape may be a suitable way to develop metabolomics approaches to study different aspects of biology, in particular specific adaptations, in relation with development and/or metabolic processes for instance. In order to integrate data produced at the transcriptome and the metabolome level, correlations between genes on one side and metabolites on the other side can be searched for. It is important to determine co-expression of genes and co-expression of metabolites separately, in order to group genes and metabolites according to similar patterns of expression or production, respectively. In this way, unknown genes will cluster with genes encoding proteins of known function (gene-gene correlations), and similarly, identified metabolites will group with metabolites of unknown structure (metabolite-metabolite correlations). It is possible to conduct similar types of analyses if proteome data are available for corresponding samples. Then it will be relevant to make the link between genes coding for well characterized proteins identified in gene expression clusters and well-defined metabolites contained in metabolite clusters, and carry on analysis in order to decipher metabolic processes. The co-occurrence of related transcripts and metabolites assessed by multivariate analysis may be a useful approach to infer functions of genes, not only in benchmark models, but also for non-model organisms. Effectively, it is currently possible to produce, quite readily, transcriptome data by NGS (Next Generation Sequencing) that can then be integrated with results corresponding to the full suite of metabolites measured in identical sample.
Transcriptome and metabolome data can also be used directly for reconstruction of metabolic networks. However, this type of reconstruction is so far mainly based on information from the sequencing of genomes. The establishment of connections between transcripts and metabolites, and integration of these data with previous genome data, can contribute to improve annotation, in particular for genomes of exotic organisms for which the number of new genes is high. It also permits to complete and check genome scale networks and to give some indications on potential regulation. This iterative process will help predicting behavior of models in order to make some hypotheses under different types of constraints and for several applications, such as the response to changes in environmental conditions. Predictions and hypotheses will need to be tested though measurement of reaction fluxes by fluxomics approaches such as stable isotope labeling techniques [
Once pathways will have been reconstructed and analyzed, it will be possible to perform comparative and evolutionary analyses of metabolic processes among different lineages, to try to relate gain and loss of genes with different phenotypes and metabolic capabilities. This will be important to trace back the evolution of pathways, not only in marine organisms. However, in these organisms, it will help to understand why a lot of them exhibit so many peculiarities in metabolic processes, including at the level of primary and secondary metabolism. One step further will be to relate these specificities with their ability to thrive in their environment, considering also the diversity of ecosystems found in the seascape. In addition, to consider metabolic data for systems biology approaches in the framework of chemical ecology, and therefore to study biotic interactions between different types of organisms in a community, a higher level of integration will be necessary, for instance to observe how perturbations within metabolic networks of a given organism can alter the behavior of other metabolic networks contained in different organisms, belonging or not belonging to the same species.
In this review, we give an overview of recent literature published on metabolite profiling in marine systems. There is a wide and increasing variety of metabolomics applications in marine organisms, performed at different scales, either to discover molecules or to analyze a broad spectrum of metabolites; the latter can possibly be used for elucidating metabolic capabilities of a given species or metabolic responses triggered by changes in environmental conditions. Metabolites can exhibit high spatio-temporal heterogeneity, due in part to fine tuning of their production, in particular for regulatory molecules. In our opinion, one way of translating several pieces of literature leads us to say that monitoring changes in metabolite contents under variable growth conditions within an organism is like looking at the "genome in motion". Effectively, metabolites present in a sample at a given time offer a valuable snapshot of what is happening at this time in a cell or in an organism if dealing with unicellular species. To produce this comprehensive coverage, there is a need for combining distinct analytical platforms based on different separation techniques coupled with MS or MS-MS. It may also be relevant, in some cases, to complete experiments with enzymatic determinations to obtain reliable and reproducible quantification of some metabolites.
Recent developments in metabolomics enable new approaches towards the characterization of the chemically mediated interactions of organisms with their environment. Particularly in chemical ecology research, where metabolic responses to stimuli like herbivory or mechanical tissue disruption are often relevant, metabolomic techniques have the potential to be an important complement to methods used traditionally [
Finally, technical developments will be welcome, in particular for fluxomics, to monitor metabolites in a dynamic way, and for MS-imaging, to precisely localize and quantify metabolites in cells and tissues. This will strengthen applications of metabolomics in systems biology approaches to study unique metabolic pathways present in marine organisms and their regulation, and also to analyze what the metabolites are and how they act to mediate biotic interactions in marine environments. Metabolomics should also be a valuable contribution to the growing interest in marine biotechnology by providing further applications in the context of green and blue chemistry.
We would like to dedicate this publication to the memory of our friend Jean-Pierre Salaün, who untimely passed away on 22 June 2011. His enthusiasm and inspiration for science—in particular, oxylipin biochemistry—will be unforgotten.
This work is a contribution within the project IDEALG which benefited from the support of the French Government run by the National Research Agency and with regards to stimuli program entitled "Investissements d'avenir, Biotechnologies-Bioressources". The set-up of the MetaboMER platform was supported by the European Regional Development Fund (ERDF)-FEDER 33765, Région Bretagne, French Ministry for Research, UPMC, CNRS, and GIS Biogenouest.


text:
For centuries, both Asians and Europeans sought to
trade with China for the luxurious fabrics produced by the silk industry.
The wealthy enjoyed elegant luxuries such as elaborately embroidered robes,
brocade sofas with richly decorated pillows, silk tapestries and tables
covered with satin cloths.
As prosperity and trade increased, so did the quality of silks from China,
providing a look and feel of richness that no other material in the world
could match. It is said that the Romans went crazy for Chinese silks,
so different from Western fashions made of linens, furs, and wool.
Closely tied to the silk weaving industry was the ancient art of embroidery.
From the Shang Dynasty (1766 to 1122 B.C.) to the Tang Dynasty (618 to
907 A.D.), the chain stitch had been favored but was soon replaced by
the satin stitch. This freed embroiderers to use different stitching styles
and new patterns. During the Sung Dynasty
(960-1280), embroidery merged with the art of painting, and famous
masterworks were painstakingly copied in thread.
During the Ming Dynasty (1368-1644), threads other than silk were experimented
with, including human hair, spun threads of gold, or other
materials. Artisans became more regional in style during the Qing
Dynasty (1644-1911), and specific provinces enjoyed special acclaim for
distinctive technical innovation. These arts have not been lost, but are
still practiced today.
Jump to one of the following subjects or scroll down to view all sections:
Click on any image for an enlarged view
Embroidery - colorful gold fish embroidered on each side of pale green
silk, resulting in no "front" or "back" and no threads
visible from the opposite side. Displayed within a carved Chinese frame
with scroll-carved braces.
--Artifact on loan courtesy of theGerald Ford Presidential Museum, Grand Rapids, Michigan
- Hunan hand embroidery by Dr. Zhang Shanzi based on the famous painting,
"Two Tigers."
--Artifact on loan courtesy of the Ronald Reagan Presidential Library and Museum, Simi Valley, California
scene - a crane and a pine tree (symbols of longevity) crafted of barley
straw and grass. Gift to Dwight Eisenhower in 1960.
--Artifact on loan courtesy of theDwight D. Eisenhower Presidential Library and Museum, Abilene, Kansas
are currently exploring "An Ancient

subset: commoncrawl


text: When trying to install the new Unity 4.2 on a OSX 10.7.4 system, i get the following error: "The Installer encountered an error that caused the installation to fail. Contact the software manufacturer for assistance." Is it possible that 4.2 does not support older OSX versions anymore? Any clues towards a solution would be most welcome!


text:
About Mamod
Useful Links
Contact Mamod
Stationary Engines
Steam Road Vehicles
Steam Trains
Kitsets to Assemble
Spare Parts
View Order
Complete Order
Terms of Sale
©2007 Mamod (NZ) Limited
Your Kiwi Source for Mamod
All prices are in NZ Dollars and include any GST payable
Terms and Conditions of Sale apply to all purchases
The images on this site are provided only as a guide
Frequently Asked Questions
Q: How do I Order?
A: Ordering can be done via the following methods:
Use online ordering by clicking
Order Now
while browsing the online catalogue.
Complete Order
to enter your shipping details. This is the simplest method.
Email your order to us (see the
Contact Mamod
page). Remember to include your contact details and shipping address.
Phone us 021 1500-145 or (03) 3146-832 (evenings)
For all these methods you will be sent an order for payment. Once payment has been received the goods will be shipped. An invoice will be sent with the shipment.
Q: Do you have a User Guide I can look at now?
A: Download our
User Guide
Q: What long does shipping usually take?
A: For items that we stock locally, shipping should only be a few days from payment. If we don't currently stock an item, or have run out of stock, then the time will be approximately 2-4 weeks from payment. We endeavour to keep you informed at each step of the way, so that you know exactly where your model is at all times on it's journey to you.
NOTE: Rural Delivery and remote areas may take a day or two longer.
Q: What do the steam engines run on?
A: Mamod recommmends that you only burn Mamod's waxed solid fuel tablets. These tablets are perfectly designed for these machines, a perfect fit for your burner tray and they last for approximately 15 minutes. The tablets are safer and cleaner than using methalated spirits and they will not leave a residue. Two solid fuel tablets maintain the correct heating while other methods are less controlable. We can supply them to you (at cost) from this site, you will find them on every page. There are 20 tablets (10 runs, well over 2 hours) in each packet.
Q: Can I run the engine indoors?
A: The solid fuel tablets are not to be used in confined spaces or in vicinity of domestic pets.
Q: How long will they run between fueling?
A: Approximately 15mins. Always use 2 tablets, no more, adding more will not make the run longer, it will only potentially damage your engine from burning too hot. Water in the boiler lasts approximately 15 minutes. Always keep the water level in the boiler above minimum. Remove the burner tray when you have finished your run to let the engine cool and prevent depleting the boiler water level. Starting with HOT water means the session will run longer as the tablets do not take long to bring the boiler up to steam.
Q: Should I add anything into the water in my boiler?
A: No. No additives are required, just clean hot water. Before the first use of your new machine, flush the boiler with warm water. Periodically you can wash out your boiler with lemon juice or vinegar to prevent limescale. (See your operating instructions on the box or inside.)
Q: How safe is it?
A: Mamod steam models are as safe as they can be and common sense will make your experience safe and enjoyable for everyone. You must be 14+ years to run a model. There is no boiler ticket required (the boiler pressure is 15psi, low enough and safe enough to not require a licence). All engines have safety valves. There are small parts so care should be taken when small children are around to prevent accidental ingestion. The boiler becomes very hot and care must be taken; hold the engine only at the handling points (refer to your specific model's instructions with your engine). Please always handle your engine in the manner described in your Operating and Safety Instructions.
Q: Does it come in another colour?
A: There are a few models in different colours, but we have indicated this in each description. If it does not indicate a choice, then you have to stick to what you see!
Please note that images on this site are only a guide to the actual colours.
Q: Can I get parts for my old Mamod engine?
A: Please ask us about any of your old engines and we will see if we can help you get them working again. We can supply any part for any engine that is currently in production - i.e. sight glass assemblies, safety valves, driving bands, chimneys, etc.. Our site shows most of these parts and some will fit older models. Please don't hesitate to ask us and we will find out for you. You may be pleasantly surprised and have your beloved machine working again!
Q: Where are these engines made?
A: We import these engines directly from the manufacturer in the United Kingdom. Mamod was established in the 1930's and has been going strong since then. (See our History page for more details). All engines are entirely made on site at Mamod Limited, in the country that made the original full-scale working engines!
Q: How do we contact you?
A: We are contactable through the avenues displayed on our contacts page. For orders or inquiries, see the
Contact Mamod
Q: Do you accept credit cards?
A: At this stage, No. Direct bank to bank transfers are the most secure option for everyone. Credit cards are no faster to process than bank transfers, but add extra cost that we would have to pass on to you. We will accept a cheque, but will not ship until this has cleared.
Q: When are the new models comming out?
A: New listings are sent each July, so be on the look out around then!
Q: Can I do a pickup?
A: We don't allow pickups. The cost to Courier will be less than the cost of petrol and doesn't take any of your time.
Q: Can I suggest a different freight option?
A: Yes. We endeavor to get the best price and service for you and if you can suggest another method we want to hear it. You could help someone else save too.
Q: Why do the prices change?
A: We are subject to exchange rate variations. If our dollar is strong, our items will cost you less.
©2007 Mamod (NZ) Limited - Your Kiwi Source for Mamod Quality European Steam Models


text:
The Missouri Department of Transportation (MoDOT) strives to balance historic preservation concerns with the task of planning, designing, constructing, and maintaining the State's complex transportation infrastructure. MoDOT's Historic Preservation (HP) staff works to identify potential conflicts between the two and to help resolve them in the public interest. The HP staff ensures that no MoDOT job is denied federal funds or permits due to lack of compliance with historic preservation regulations. MoDOT makes every effort to comply with federal and state historic preservation legislation and regulations, and address citizen concerns, while being a good steward of Missouri's historic and prehistoric resources.
Section 106 of the National Historic Preservation Act requires that MoDOT consider the potential impacts that any federally funded or permitted project may pose to significant cultural resources. Cultural resources include archaeological sites, buildings, structures (e.g., bridges), objects, or districts. The significance of a cultural resource is evaluated by applying a specific set of criteria that are set forth by the National Register of Historic Places. Cultural resources that meet the criteria of eligibility for listing, or already listed, on the National Register of Historic Places are referred to as "historic properties." MoDOT must evaluate the potential impacts of a project on all identified cultural resources, regardless of their significance. Failure to obtain Section 106 clearance could jeopardize federal funding and permits for a project, which could result in project delays. After the review process is initiated, compliance with Section 106 requires three things:
Section 106 encourages, but does not mandate, the preservation of historic properties. The goal of Section 106 is to ensure that preservation values are factored into the planning process for all federally funded or permitted projects. It provides assurance that agencies will assume responsibility and public accountability for their decisions when dealing with cultural resources.
The Advisory Council on Historic Preservation's pamphlet Protecting Historic Properties: A Citizen's Guide to Section 106 Review is an excellent starting point for citizens seeking more information on their role in the Section 106 process. The Advisory Council on Historic Preservation, as per their website, "…is an independent federal agency that promotes the preservation, enhancement, and productive use of our nation's historic resources, and advises the President and Congress on national historic preservation policy." In Missouri, the State Historic Preservation Office in the Department of Natural Resources is responsible, in partnership with the U.S. Department of the Interior's National Park Service and local governments, in assisting in the Section 106 process.
If you have concerns or knowledge of a proposed MoDOT project that may impact a historic property, please contact MoDOT's historic preservation manager, firstname.lastname@example.com.
Links to the historic preservation topics that MoDOT considers in the planning, construction, and maintenance of Missouri's transportation system are located at the top of the page.


text:
I am using an old DOS-based program which is very old, but very convinient
for me to use. In Windows XP, I was able to get around this problem by
using the following commands:
net use lpt1 \\violin1\brother /persistent:yes
where "violin1" is the computer name and "brother" is the printer name.
See the following link:
I have also verified using the "net view \\violin1" command that "Brother"
is being used as LPT1 for Printing"
However, when I try to use the print command within the DOS program, nothing
happens. No errors, but just sits there until I manually close the window.
I am running my old DOS-based program to be compatible under Windows 95.
Anyone with ideas as to why this is not working with Vista Ultimate?


text:
Here are the answers to some questions we've been asked.
If your question isn't answered here feel free to contact us.
Our probes tried to visit your site via each mobile and home-broadband network we monitor, but instead of returning the page as normal, some or all of the ISPs sent a page telling us their filters have blocked the site.
Each test line is configured with its default level of filtering. The results you're seeing are what the Government would like each ISP to nudge their customers into using.
If you're seeing a lot of timeouts it could be that the site you're testing uses TLS with Perfect Forward Secrecy. This increases the length of time between requesting the site and its content being returned. We're looking into tweaking our probes to detect this and allow a little longer for the test.
BT's blocking is a bit of a blunt instrument - if you try to connect to a site that is blocked using HTTPS, you'll get a timeout. The proxy server that their DNS hijacking directs your request to doesn't respond on port 443.
Unfortunately, no. At the moment, we've chosen the "default" choice for filters on ISP networks. Some ISPs allow account-holders to filter much, much more. Hopefully we will be able to test for stricter filters in the future. We also can't test all filters everywhere, as many people are on private networks. We hope to get more information about filters in schools, workplaces, libraries and on public wifi networks in the future.
As of October 2016, we are having problems with our Three probe. Three only provide filtering on their mobile network, while our probes work best using data SIMs. We hope to resolve this as soon as possible.
You will see results from EE where these had been created before we took our EE probe offline.
The filtering settings on your network might be different to those we've configured on our test lines. Here are some other possible explanations :
If we're monitoring that network then our probe might be offline or under heavy load. Please try again later.
You can subscribe to updates about a website when making a check via the form, but we only make intermittent checks. We can make much more regular checks, but this would likely to raise our costs. Contact us if this would be useful to you.
We are always very willing to bulk test data. We can take submissions through our API or via CSV files. Everything we test usually becomes available under our published data. Get in touch with us.
If you have access to a filtering system you want tested, contact us so we can arrange to run a probe to run tests.
Yes, you can download the full dataset from our licence page.
Yes. The government estate (schools and hospitals, for instance) blocks what they believe to be "extremist material." UK courts have ordered blocks on some websites that enable copyright infringement. A handful of URLs are blocked by members of the Internet Watch Foundation for containing child abuse material.
EE, O2, TalkTalk and Vodafone all came with their default "adult content" filters enabled by default. The following ISPs provided no filtering by default: 3, Andrews & Arnold, BT Broadband, Plusnet, Sky Broadband and Virgin Media.
Before we launched the site we tested the top 100,000 sites (as ranked by Alexa at the time) on each of the networks we monitor. We found that just under 19% of these were blocked on at least one network.
There's a detailed information page on Wikipedia. This page also has a list of site categories blocked by different ISPs in different circumstances.
We have test lines from 3, Andrews & Arnold, BT Broadband, Everything Everywhere, O2, Plusnet, Sky Broadband, TalkTalk, Virgin Media and Vodafone.
Andrews & Arnold sponsor this project. They also offer only unfiltered connections so they are a yardstick against which to compare other providers.
We chose BT Broadband, Plusnet, Sky Broadband, TalkTalk and Virgin Media because these are the ISPs the Government has pressured into agreeing to install "active choice" adult-content filters for new and existing customers. Together they provide over 90% of UK home broadband connections (according to the thinkbroadband UK Broadband Factsheet, Q1 2014, P3).
We chose 3, EE, O2 and Vodafone because these are the only licensed Mobile Network Operators (MNOs) in the UK. All have default-on filters. Other companies such as GiffGaff, Virgin Mobile and Tesco Mobile are known as Mobile Virtual Network Operators, or MVNOs. The MVNOs all use the infrastructure of one of the four MNOs to provide their service. As part of this, we believe they also inherit the parent service's filtering infrastructure, though they can choose whether to make this available to their customers. So MVNOs either have no filtering or the same filtering as one of the MNOs. That means we can cover the entire UK mobile market by monitoring just the four MNOs. Wikipedia has a list of UK MVNOs and the MNO that provides service to each.
Andrews & Arnold do not offer filtering so we couldn't enable any.
BT Broadband filtering is set to "light", which they say blocks the following categories:
PlusNet do not offer network-level filtering so we couldn't enable any. We have enabled "Safe Surf" and "Broadband Firewall" though.
Sky Broadband filtering is set to "13" which they say is "suitable for teenagers and above". They say this blocks the following categories:
TalkTalk KidSafe is enabled. We have also enabled "Virus Alerts". The categories they say KidSafe blocks are:
Virgin Media's parental controls are set to "on".
We asked 3 to enable adult-content filtering. They said they'd try but it might not work. 3 switched off default filtering for contract data-SIMs in March 2011.
Everything Everywhere's Content Lock product is enabled.
O2 block "18+ rated websites" by default. This is enabled.
Vodafone Content Control is enabled.
The probes look for the messages that ISPs return instead of the page you asked for when they've blocked a site. If a probe doesn't see a "page blocked" message, and there was no error, then it assumes what was returned is the normal version of the site and reports "OK". This might mean we're missing some forms of censorship, or might start to do so if an ISP changes its "blocked page" message. If you think that's happening please contact us and let us know.
User submitted sites are checked straight away and the results are normally available within a few seconds.
We send user-submitted URLs, and the Alexa 100k top-ranked sites, to a re-check queue at a rate of 4800 per day (in last-checked order). User-submitted URLs must be more than seven days old to be requeued. With the current database contents, this means we re-check the entire list every 20 days. Re-check URLs go to a different queue from real-time user submissions so they don't get in the way of your results being returned.
Please see the credits page.
See the get involved page for practical ways you can help, such as analysis, coding, campaigning and joining ORG.
We have plans to allow this in future. Right now we're developing a few different probes, including an Android app, a GNU/Linux command-liine probe and integration with ooniprobe. We'd also like to build a browser extension. If you want to help with any of these projects please take a look at our get involved page.
Yes. our code is Free software and we would be very willing to help you set up your own version, or collaborate on a central repository. Ooni from Tor is also Free software and we are working on integrating it with our system. Please contact us if you want to explore further.
Yes, you can download a snapshot of the data from 29 June 2014, or a nightly update, licenced as described on our licence page.


text:
25 items found in this collection
"Are you sure it will launder?"
"Pretty" - Yes, but so impossible to KEEP pretty!
Once there was a young bride...
"Another clean dress"
Wooly as little Teddy Bears!
Mistakes women make in the care of their complexion
How to fight the little foes which work to mar your skin
How to overcome the havoc wrought by sun, wind and dust
Two important sign posts that point the way to beauty
Small mistakes that bar the way to beauty
How to fight the little foes which work to mar your skin
Beware of the little flaws that make one homely
Before Powdering
Every normal skin needs two creams
Every normal skin needs two creams
What wind and cold do to the skin -how to protect it
Page 1 of 2 Next »


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Would you like to save as your currency permanently or for this visit only?
Old cape feel. Low key and lovely location on the dune overlooking province town. Outside province town which was good for me.
Clean, great location Very dated. Floors had rot holes under carpet, felt like I could fall through.
Price /location Wifi did not work well away from office and I signed up for a cheaper room and ended up with something more expensive which would be fine but wifi is spotty
Excellent location. Easy access to High Head Beach, P-town and biking. Friendly, helpful staff. Great views. The room we had was part of a row of rooms built up off the ground, so if someone walked around their room, the vibrations would come through to ours ! Best to have a room on the ground ( which they have ).
Had air conditioning Customer service was kinda mean Room was cramped Wasn't near walking distance of anything fun
Everything you would want right there. Beach, kayaking, pool, pool table/ping pong table, etc. Suite has a single bedroom with kitchen/living area separate which was nice. Staff was very helpful. location was far enough away from the hustle and bustle but just a short drive into town.
beach area - filthy water and sand, no place to sit ; wifi reception spotty, NO AC !!!


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|Luminous Animal (1000+ posts) Send PM | Profile | Ignore
|Sat Jun-04-11 09:28 PM
Response to Reply #12
|42. Sorry. I assumed your kids went to public school and you are OK with the govt. testing them.
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compareit4me.com™ – saving you money one click at a time
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April 2014:
We signed our first round of investment with a group of angel investors led by Jonathan Hall of Mulverhill Associates.
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September 2015:
We announce the news we've raised $3 million in VC Funding. The funding was led by STC Ventures, the VC arm of Saudi Telecom, with backing from Wamda Capital, which is headed up by Aramex founder Fadi Ghandour, and Dubai Silicon Oasis Authority completing the investment group
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So, that's us! Whether you want to apply for a home loan, switch bank account providers, pay for a major life event, finance a new car or find the most suitable credit card, compareit4me's sophisticated search engine and smart comparison tools ensure you can secure the best financial product for your needs. You'll also stay informed about what's going on in the banking world thanks to our easy to read (and understand) articles.
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So, that's us! Whether you want to apply for a home loan, switch bank account providers, pay for a major life event, finance a new car or find the most suitable credit card, compareit4me's sophisticated search engine and smart comparison tools ensure you can secure the best financial product for your needs. You'll also stay informed about what's going on in the banking world thanks to our easy to read (and understand!) articles.
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- compareit4me's Guides & Advice section is the UAE's leading financial resource platform with extensive articles to educate and inform readers, simplifying sometimes complex topics for all to understand
Enough about us…start comparing those finance products!


text:
Plant Patents
A person who invents or discovers and asexually reproduces a distinct and new variety of plant, including cultivated sports, mutants, hybrids and newly found seedlings, other than a tuber-propagated plant or plant found in an uncultivated state, may obtain a patent, subject to the federal laws governing patents for inventions. The description of the plant in the patent application must be as complete as reasonably possible. A plant patent grants the right to exclude others from asexually reproducing the plant or selling or using the plant so reproduced. Plant patents have a term of 17 years from the date of issuance.
Contact our office today to find out how we can help you. Call (617) 651-1150 or email us today to schedule your free, no obligation initial consultation.


text:
In a season filled with pitching uncertainties Darren Oliver has been a beacon of consistency for the Toronto Blue Jays.
Now, at the age of 42, it is the left-hander who is uncertain if he wants to continue his Major League career another season.
"I'm not thinking about it right now," Oliver said on Sunday, when asked if he is up to another year. "I'll worry about that when the season's over, when I'm at home, relaxing. You guys catch me in the off-season somewhere. See what happens when I'm in the Bahamas."
With Blue Jay pitching staff a dicey proposition all season, either through injury or sub-par performances, Oliver has been one name that manager John Farrell has been able count on to get the job done.
In this, his 19th Major League Baseball season, the reliever has held opponents to a .208 average (41-197) and allowed a run in just nine of his 60 games. On September 16th in Boston, Oliver recorded his team-leading 15th hold of the year.
Oliver knows what it takes to win as this will be the first time since 2006 that teams he has been a part of have failed to make the postseason. And the Blue Jays would dearly welcome him back if he is willing.
"I've kind of been spoiled over the past few years," Oliver said. "Like they say, every good thing always comes to an end. Teams are going to make the playoffs and teams aren't. I never took it for granted, the past years when we did make the playoffs.
"Everybody in this room, once they get a taste of it, they're going to know what's it like to be there and always want to grind to get back."
Oliver has two children, aged 10 and 12, and their well-being will factor into his off-season decision on whether to continue or not.
"My two kids and my wife," Oliver said. "I would never play this game and be away from them and take that away from them. Every kid needs a father at home. That's important to me. I think it should be important to a lot of people. Baseball's second. Family's first. I've always said that."
Farrell said he is not really in a position to try to influence Oliver's decision one way or another.
"That's one of those areas only that person truly knows," Farrell said. "Because he's performed so well you all of a sudden assume, that, hey, there's a lot of good pitching left. I'm hopeful that he'll say yes he will continue, for obvious reasons – the way he performs and the way he handles himself and the steadying force he is with the team.
"On the flip side, is that player wondering should he go home, pursue other things. And the question is, what is your gut telling you. And if there's ever any uncertainty then you say keep going. That has to be exhausted. "Report Typo/Error
Follow us on Twitter: @Globe_Sports


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Remember picking blackberries as a kid?
Trying to keep from being stuck by the thorny bushes, watching out for snakes, wasps and other unwelcome critters, and baking under the summer sun? Your sweet reward — a big bowl of plump, purplish-blackberries sprinkled with sugar and swimming in milk or cream — was worth the effort.
Now, you can walk into many grocery stores' produce departments and see displays of delicious summer berries, including blackberries.
The childhood favorite is cultivated across the United States and available into September. Look for deep-colored berries without hulls. Fresh berries are best if used right away, but can be refrigerated for up to two days.
"You don't have to wait for dessert to get the fresh taste of berries," says the Oregon Raspberry & Blackberry Commission. Add berries to salads, such as this refreshing and easy-to-prepare, main-dish Tuscan Quinoa Salad. The commission also offers a twist on the classic shortcake dessert. Try Blackberry Almond Shortcake, which is loaded with juicy blackberries and topped with the crunch of almonds.
Both recipes work with either fresh or frozen berries.


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MOJEFFER-L ArchivesArchiver > MOJEFFER > 2000-10 > 0971525700
Subject: [MOJEFFER] YATES, CHARLES & JONAS
Date: Sat, 14 Oct 2000 08:15:00 EDT
I believe that Jonas YATES of Jefferson Co.,MO is a brother to my Charles
born ca, 1792 in KY of Washington & Jefferson Counties, MO. MY line is
Charles 1792/John Jonas 1826/Susan 1856/Leona 1879.
Mary Clarissa YATES, d/o Jonas, married Elias YATES, s/o Charles & Samuel
YATES, s/o Jonas, married Lucinda YATES, d/o Charles.
The COULTER & MYRICK surnames do not appear in my records of these families,
so I'd like to exchange data.
Barbara Anne
This thread:


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AUGUSTA, Maine — For most Soldiers, packing up the old olive drab colored duffle bag and preparing for deployment is just a fact of life. Some eagerly throw their packs over their shoulder and never look back, and there are some that do look back and say good-bye to the family they are leaving behind.
When the 488th Military Police Company deploys to Afghanistan this summer, spouses and children will be taking on extra duty and trying to keep their deployed spouse present despite the distance.
Staff Sgt. Angela Baker, training non-commissioned officer for Joint Forces Headquarters and Headquarters Detachment, is no stranger to deployment. She left her first child Gavin at eight months old for her first deployment to Iraq in 2004.
Now, she prepares for her husband, Sgt 1st Class Brian Baker's second deployment away from her and their two year old son Jacob. She plans to help herself get through this one the same way she always has, by staying busy.
"I didn't spend my day's home alone worrying, because I was too busy," said Baker. "Holy crap the kids hungry again. He's awake, got to get formula. He's out of diapers. How can he be out of diapers, I just bought diapers?"
"It's never dull and the kids kept me busy, it made it ok," said Baker. Part of being busy will include sending emails and using video chat to stay connected to Brian.
Baker wants to use phone and video chat so Brian can see the boys and Jacob doesn't lose that interaction with his father. "He is at an age where I am not sure what he will do, he knows who his dad is but I would hate for him to lose any recognition."
Thanks to modern technology Soldiers have an advantage that they haven't in the past. Families of the 488th will have the chance to video conference the Soldier home for Christmas morning and birthday parties.
Along with on-line chat Sgt. Michael Bachman, senior human resources non-commissioned officer for the MP's, will use additional resources to make sure his twelve month old Dante, hears and see's daddy.
"We are getting two flat soldiers." Torso sized cut-out photos of the deployed Soldier that families can bring to parties, ball games and just have around the house. "We are going to tape videos of me singing lullabies because that's what my son's used to, we are recording books and my wife is going to hang a lot of pictures so he remembers my face," said Bachman.
Communicating with his wife Annette is just as important as his son explained Bachman, "It lets them know they're still thought of, I am not running away from her or my responsibility as a father. I will let her know that every day."
The MP's, known as the Guardians, have made it a priority to have a strong Family Readiness Group to ensure the family left behind is well supported and the unit downrange feels more comfortable.
Sgt. Travis Young, a military police officer with 1st Platoon, 1st Squad is impressed with the unit family support and knows they will be there to assist. "Because we have such a good support group that is going to help our family, it will help me concentrate on the mission and help me get the job that I have to do done.
Having that kind of support is very important for Baker. "The one thing that we share, and say we as the human race, is commonality. When we find people we have something in common with especially during a tough time or a big transition in our lives, we bond together. With the FRG, family days and Yellow Ribbon events, you know you're not going to go through it alone. The best thing to do is go ahead and make those connections, because if you are feeling it someone else probably is to.
When the Guardians do sling their duffle bags on their shoulders and head overseas they will be far from the house, but still close to home.


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Clinton Kelly's Leftover Lamb Samosas with Mint
Give your lamb roast a leftover makeover!
- Ingredients
- step-by-step directions
Clinton Kelly's Leftover Lamb Samosas with Mint
- 2 cups Roast Lamb (chopped into small pieces)
- 2 cups Minted Peas
- 2 cups Mashed Potatoes
- 1 Package Storebought Pie Dough
- 1/2 Onion (minced)
- 1/4 cup Mint Leaves (torn)
- 2 tablespoons Extra Virgin Olive Oil
- Vegetable Oil (for frying)
- In a large sauté pan over medium-high heat, heat the olive oil and add the onion. Sauté until softened, then add the lamb, and peas. Once warm add to bowl with mashed potatoes (should have equal parts lamb, peas and potatoes). Add the mint and fold together. Set aside to cool slightly.
- Meanwhile, on a floured work surface, using a 6-inch ring mold, cut out circles of dough. Cut the circles in half.
- Spoon a small amount of the filling into each of the half moons. Fold the dough and filling over into a cone and then seal the end with a dampened finger.
- Preheat oil to 360F.
- Fry each samosa until golden brown, about 4-5 minutes. Transfer to a paper towel lined plate.
- Serve with mixed greens with yogurt vinaigrette.
Similar categories: Dinner Courses & Meals Lunch Asian Cuisine
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text:
Official royal wedding news alert! Prince Charles has issued more wedding details for Prince William and Kate Middleton's big event on April 29.
Kate has asked her sister, Miss Philippa - "Pippa" - Middleton, to be her maid of honor.
And Prince William has asked his brother, Prince Harry, to be his best man.
(The big question now: Will girlfriend Chelsy Davy be invited to the wedding? Harry and Chelsy were spotted having a pizza together on Friday night in London, fueling buzz that the on-again, off-again two are on again.)
William and Kate have chosen the following bridesmaids and page boys (these are their ages on wedding day):
Unfortunately, we won't see the bridesmaids' dresses and the page boys' uniforms until the wedding day, says Charles.
Sign up for Lifeline Live e-mail alerts


text:
Perveiz Ali
Mother - Poem by Perveiz Ali
No doubt a human yet unlike any other,
who can dare to campare with the another.
Showing love, tenderness and concern,
Unique to each child each in turn.
With sympathy, empathy and compassion,
An epitome of maternal love with distinction.
Many are alone and are the wolves fodder,
Reality to me is I have a dotting mother.
A teacher, a builder of nation for the better,
Real and loving with courage mixed in her batter.
A promise to seek welfare for her brood,
Comes from a mother, she finds us our food.
To struggle, strain and sweat in hard labor,
Just to see us smile and happy as no bother.
She sacrifices her leisure for her offspring,
Knowing full well treasures it will not bring.
Compromise her comfort to ensure our,
A mother will slave and fight for hours.
Our sheild and protecter through formative years,
She takes it all in strife, always hiding tears.
Topic(s) of this poem: love
Comments about Mother by Perveiz Ali
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Perveiz Ali's Other Poems
Famous Poems
Phenomenal Woman
Maya Angelou
Still I Rise
Maya Angelou
The Road Not Taken
Robert Frost
If You Forget Me
Pablo Neruda
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Annabel Lee
Edgar Allan Poe
Caged Bird
Maya Angelou
Rudyard Kipling
Stopping By Woods On A Snowy Evening
Robert Frost
A Dream Within A Dream
Edgar Allan Poe


text:
Mental Health
Have Mental Health Questions? Ask a Psychiatrist Online
Good morning!
Thanks for writing to JA.
To begin, you should know that Narcissistic Personality Disorder (NPD) is somewhat controversial. You should know several things about the field of psychology/psychiatry:
1. The "big book" used for diagnosing mental disorders is the Diagnostic and Statistical Manual of Mental Disorders... and has undergone a number of revisions and changes over the years.
2. While there are still discussions and arguments about the material contained in the DSM, none is more controversial than those disorders located on Axis II (so-called "Personality Disorders").
3. You should understand that there are licensed mental health professionals who do not believe in the concept of "personality" and do not, therefore, believe that a personality can be disordered.
4. Even for those who find sufficient evident for "personality," the disorders listed on Axis II can be controversial. Besides difficulties with diagnostics, if personality is an immutable trait of an individual - how can one treat it? If one were to diagnose an individual's entire personality as "disordered," what could one do to "re-order" it?
The reason you are probably finding difficulty locating help dealing with narcissism (NPD) per se is because of the controversy surrounding the concept of the disorder. Simply put, most licensed mental health professionals would like find other diagnostic categories to describe your BF's behavior. Certainly an Axis I disorder would be "more treatable" with a better prognosis than an Axis II disorder. Axis I disorders also tend to be better researched and have better developed treatment protocols (either medication or therapy).
Research has consistently demonstrated that treatment plus medication is more effective than medication alone or treatment alone for a majority of Axis I disorders. Further research has shown that Cognitive Behavioral Therapy (CBT) is the most effective form of psychological treatment/intervention.
A licensed psychologist/psychotherapist with specific training in CBT modalities would be able to address your concerns. I would encourage you to find a licensed mental health professional with whom to work, employing CBT. Referring to a psychiatrist for medical treatment is appropriate if you are interested in pursuing this approach.
Again, the mantra of years of research says: medication or treatment alone is not as effective as both working in tandem. Some research has also indicated that insight-oriented talk therapy is counter-productive with some forms of Axis I disorders... it actually exacerbates the condition(s). So, seek out a CBT therapist who will provide targeted, efficient, and effective therapy - not someone who signs you as a "lifer." If you're going to a therapist for years, something about the therapy isn't working.
SO: I will return to an adage I learned during my residency... "If you hear hoof beats bearing down upon you, it's far more likely to be a horse than a zebra that's coming..." The same is true with mental health disorders. NPD is highly controversial, very rare (if it exists at all), and some would argue, untreatable. A host of other disorders (that *are* treatable with good prognoses) could explain the very same behaviors used to describe NPD.
Knowing that you care for your BF, and want the best for him - I would encourage you to work with licensed mental health professionals who specialize in horses, rather than zebras. You deserve (and he deserves) solutions to his problems... and I believe the first step you might take is getting an objective, scientifically-based evaluation from a licensed mental health professional - preferably someone with a CBT background.
Thanks. I hope you're well and that this was helpful.
* FEEDBACK ENCOURAGED. Please contact me prior to leaving negative feedback so that we can resolve the matter. I am eager to work with all JA clients to provide them with useful/helpful answers. Thanks again.


text:
The rankings after the Australian Open are out and
David has moved up one spot to #86.
Today, the tennis world may still revolve around Melbourne and the impending final of the Australian Open. But for David, it will soon revolve around the picturesque little town pictured here - Bamberg.
Probably next Friday, the Argentine team will be on its way to Germany for the first round of the Davis Cup.
For David, it's going to be the first tie under the captain he wanted for this job for years, his former coach
Martin Jaite. Who, despite his experience as player, coach and tournament director is new to this job and also to this kind of scenario, unlike his players:
It's not the players who are making their debut here, it's the captains [Jaite and his vice-captain Zabaleta]. (Source.)In the same interview Jaite also said that although he's of course aware of the pressure it means to play for Argentina, he still wants the Davis Cup to be fun for the players. Something they really want to do and enjoy doing. Well, I guess he can count on David for that...
On the German side, Florian Mayer has started training again (after his injury problems that forced him to pull out of the Australian Open) and therefore it looks like he's going to play, with Philipp Kohlschreiber, and Philipp Petzschner and Christopher Kas for the doubles completing the German line-up.
From the Davis Cup now over to something completely different. As you've probably seen, Megaupload, the site we used to upload matches for David on Screen has been shut down. And I'm afraid, this also means the end of David on Screen (more about it on the David on Screen page). The question now is whether it should be replaced with something else, a different kind of extra page. I'm currently thinking about this but I'd like to hear your ideas.
So - what do you think? What kind of page would you like to have?


text:
Sharon may ignore 'no' vote
Page Tools
Israeli Prime Minister Ariel Sharon could still pursue his proposal for withdrawing from the Gaza Strip even if his party rejects the plan in a referendum on May 2, according to a senior Government official.
In the continuing violence in Gaza on Thursday, Palestinian doctors said two girls, aged 4 and 11, and a 16-year-old boy were killed.
Mr Sharon has urged the Parliament to back his proposal, saying US President George Bush gave Israel several important assurances last week concerning future political arrangements. Israel should not risk losing that support by failing to deliver on its part of the bargain, he said, adding: "Since the establishment of the state, we have not received such vast and staunch political support as was expressed in the President's letter."
Senior members of Mr Sharon's cabinet have agreed to back the plan after earlier expressing reservations. Last month he said he would abide by the result of the Likud party referendum but an opinion poll in the newspaper Haaretz on Thursday suggested that the vote among the 200,000 Likud members would probably be close, with 44 per cent supporting the plan and 40 per cent opposing it.
Mr Sharon told legislators that a vote against his plan would not necessarily derail it. He characterised the party ballot as "a public and moral duty, not a legal or binding duty". A senior Government official said the party vote would have no constitutional weight. Mr Sharon could still present the plan to the cabinet and the legislature for formal approval. Those bodies appear likely to support him.
In this week's Gaza violence, the Israeli military said it used live fire against gunmen and responded to stone-throwing youths with rubber bullets and tear gas. At Kamal Adwan Hospital, Dr Mahmoud Alaa Assala said an 11-year-old girl killed in the violence on Thursday died from shrapnel wounds. A four-year-old girl died after inhaling tear gas. The Israeli military said the soldiers did not see any small children.
In a separate development, an Israeli human rights activist has released a photo showing a 13-year-old Palestinian boy tied to the front of an Israeli police jeep.
The boy, Muhammad Badwan, was detained by the Israeli border police on April 15 in the West Bank village of Biddu, according to Rabbi Arik Ascherman, the head of Rabbis for Human Rights. He said the boy was kept on the jeep for at least two hours to discourage Palestinians from throwing stones at the police.
The Palestinians were protesting against the building of the West Bank barrier that will separate Biddu residents from their farmland. Israeli police said the case had been sent to the Justice Ministry for investigation.
- New York Times


text:
PLEASE NOTE: Your web browser does not have
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| Glossary
1. Person, generally in apparent good health, who harbours organisms that can infect and cause disease in others
2. Person who has one copy of a recessive disease gene but is not affected themselves
©2001 -


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Southmoreland baseball parents plan golf outing
The Southmoreland Baseball Parents Organization was looking for ways to raise money for the junior varsisty and varisty squads.
It still has its annual T-shirt sales and ticket sales for Pirate games, but this year something different is on the docket.
The organization will sponsor a golf outing Sunday at Timber Ridge Golf Course in Mt. Pleasant. Check in is at 8 a.m., with the golf tournament set to begin at 9 a.m..
"We have a golf tournament going on," said Marty Ridenour, organization president. "We're doing some raffles, we're doing some contests. It's a local tournament. We're just asking golfers in the area to come out and support us a little bit."
The cost for individual players is $75 and $275 for a foursome. There also are sponsorship opportunities available ranging from $50 to $100. The price includes cart and fees, dinner, one alcoholic beverage coupon, driving range, competitions in hole in one, longest drive and closest to the pin, along with a basket raffle and other prize opportunities and an awards ceremony. The event will take place, no matter what the weather is Sunday.
"All the parents will be there to help us," Ridenour said. "We've got a nice set of golf clubs to raffle off. We're not too knowledgeable about how to do this. The golf club is helping us along. We're playing it by ear."
Donations and funds raised help fund the cost of clinics, training, equipment, fall ball umpires and the end of the year banquet.
"Whatever the team needs or the school needs, we try to provide a little extra for them," Ridenour said.
Ridenour had two sons on the team in 2013 — senior Mitch and sophomore Max.
"We support both the JV and the varsity program," Ridenour said. "Our major fundraiser in the past has been just some T-shirt sales and 50-50 tickets, things of that nature. It didn't raise a whole lot of money. This year we decided to do something a little bigger. We're rookies at this. We've never done it before. We're just hoping for the best."
Ridenour added that selling the tickets to Pirates' games can be a difficult fundraiser. The tickets they have for sale this year are for games May 31 and Aug. 16. May 31 just happens to be the date of Southmoreland's graduation, making the task a little harder.
"We are hoping the folks that can use (the May 31) tickets will purchase them," Ridenour said. "We're designating Aug. 16 as Southmoreland night and we're hoping to get as many people from the local area to come on down and watch the game and have a showing for the team."
But for now the focus is on Sunday and a first-time golf tournament to raise funds for the Scotties' baseball program.
"We're hoping to raise some funds and see the community come out and support us," Ridenour said. "We're trying to plan a nice day for all involved,"
Information on sponsorship and registration is available at www.southmorelandbaseballfundrasiser.com.
Paul Paterra is a staff editor for Trib Total Media. He can be reached at 724-887-6101 or firstname.lastname@example.com


text: The Oculus Rift headset, which features a fully immersive virtual reality experience, originally made its debut on Kickstarter last summer where it raised almost $2.5 million. Since that time it's been in development and while a dev kit is available, the initial consumer version is expected to debut next year. As the dev kit is only compatible with the Mac and the PC, there's been speculation about whether or not the Oculus Rift will work with next generation consoles, like the upcoming PS4 and the Xbox One, but it turns out that's not what the company is aiming for. According to Oculus Rift CEO Brendan Iribe, who spoke with Edge, the company isn't planning to develop the headset with upcoming consoles in mind, and instead plans to focus its efforts on both PCs and mobile devices. As our sister site TouchArcade points out, mobile devices like the iPad and the iPhone are fully capable of supporting the headset's 1080p display and the technology exists for a potentially wireless system using Bluetooth and AirPlay. With the addition of MFi game controllers, mobile gaming could be entering a whole new era with the Oculus Rift. Iribe wasn't able to give a specific release date for the Oculus Rift, but he said that the company "would love for it to be next year." Article Link: Oculus Rift Virtual Reality Headset to Support 'Next Gen Cellphones'


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Canon has been running an annual themed photography competition in Australia and New Zealand for the last couple of years called Photo5. It's good fun, and gives you the challenge to stretch your photography in a direction you wouldn't normally go.
I've entered that comp for the last 2 years. I've imagined that my photos are good enough to run away with a 5D MkII and a photography trip, but alas, nothing has come of it. My imagination is better than my photography!
But I'm glad that Dreamstime isn't run by Canon, and that the Canon judges are not Dreamstime reviewers. The judges selection of finalists is, in my opinion, pretty bad. There were many fantastic...
Stephen Denness
Cranbourne North, AU
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Cruise with family: Oasis of the Seas Cruise Review by sometime_cruiser
Overall Member Rating
Cruise with family
Destination: Eastern Caribbean
Embarkation: Fort Lauderdale (Port Everglades)
- Embark and disembark were super efficient. Kudos go to RCL to solve this problem.
- Very clean ship. All the public places were free of debris.
- Very clean stateroom. No foul smell during our entire stay.
- Very clean beds, sheets and pillows.
- Very clean bathroom in stateroom. We always closed the lid on the toilet to minimize/eliminate odor.
- Large venues for shows with enough standing room.
- Shows were mostly enjoyable, except we found Hair Spray to be quite long.
- Dining was very good. We only used the complimentary dining services but we found the food and service to be very good.
The dining staff cleared the dirty plates from tables quickly, except in Windjammer at peak lunch hour.
I really liked the Solarium for lunch (light but tasty),
Park Place for breakfast and Opus for dinner.
Windjammer was More also OK (plenty of buffet food) but nothing to sing about.
You can't go hungry or thirsty on the Oasis and indulge all you want at zero cost:
Coffee from Park Place, Cafe Promenade, Windjammer.
Juices in Park Place and Windjammer.
Baked stuff and sandwiches from Park Place, Cafe Promenade, Donut shop, just to name a few.
Pizza from Sorrento.
Burgers & fries from Wipeout or Windjammer.
- Interactive TV in stateroom: check your account, check dining room capacity, show time, book excursions etc. Very convenient. Although the speed of screen update was very slow you thought you were using dial-up modem.
- The drink server were not pushy to sell you drinks at the pool decks or at the show venues. On other cruise line, I got sick of saying "No thanks" to the drink servers so I hope RCL doesn't become more aggressive to sell more drinks.
- Crowd: We expected it would be crowded but we were surprised by how crowded it was.
On embarkation, we went up to Windjammer for lunch at 12:30pm. It was a mad house with scarcely any empty table. On other days, it was not as bad but you would have to be quick on your feet to nab an empty table to fit a family.
Most of the times except late night, the elevators were crowded and my family usually had to wait for another car to come.
The worst wait time was obviously meal time so we decided to walk to/from our dining rooms (it was a good exercise to burn off the calories :-))
On sea days, it was difficult to find empty chairs on the pool deck. Either you get to the pool real early or real late.
- Small state room:
We had large suitcases and had to stow them under the beds to leave enough space to walk around the state room.
But of course, we had to pull the suitcases from under the bed if we wanted to get some thing. This pull-out/push-in became pretty old very quickly.
- No opened liquid container allowed after port calls: I was not sure why but we couldn't bring opened water or soda bottles we bought from the ports onto the ship.
- Photographs were expensive: $20 for an 8x10 sheet and $10 for smaller. You could spend several hundred dollars on pictures alone. Less
Published 06/19/11
Cabin review: M Large Interior StateroomLarge Interior Stateroom (M) Reviews >>
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Associated Press
OAKLAND, Calif. — Down and dominated during most of the first two games at home, the San Antonio Spurs once again showed why nobody should ever count them out.
Tony Parker scored 25 of his 32 points in a sizzling first half, Tim Duncan added 23 points and 10 rebounds, and the Spurs quieted the Golden State Warriors and their rowdy sellout crowd in a 102-92 victory Friday night to take a 2-1 lead in the Western Conference semifinals.
"We made shots and they didn't have as good a night shooting the ball," Spurs coach Gregg Popovich said. "Sometimes it's as simple as that."
Kawhi Leonard finished with 15 points and nine rebounds, and Parker added five assists and five boards to help the Spurs wrestle back home-court advantage from the Warriors after withstanding a brief fourth-quarter rally.
San Antonio outshot Golden State 50.6 to 39.3 percent and curbed streaky shooters Stephen Curry and Klay Thompson for the first time in the series.
Thompson scored 17 points on 7-for-20 shooting, while Curry had 16 points while going 5-for-17 from the floor. Curry sprained his left ankle again in the final minutes. He was icing his ankle in the locker room and his status moving forward is unclear.
Andrew Bogut added 11 points and 12 rebounds for Golden State but was saddled with foul trouble most of the second half. Game 4 in the best-of-seven series is Sunday in Oakland.
After falling behind by 10 points entering the fourth quarter, the Warriors scored the first nine of the period in fewer than 2 minutes. They capped the run by slicing San Antonio's lead to 79-78 when Bogut blocked Duncan's layup, and Draymond Green started a three-point play by drawing a foul on Duncan with a pull-up jumper.
Parker put San Antonio back ahead by seven with a 3-pointer before leaving briefly with an apparent left leg cramp. Trainers wrapped his leg while he was on the bench and he showed no signs of slowing down when he returned.
With Parker on the bench, Duncan converted a three-point play and Leonard added a layup to cap an 11-1 run that gave San Antonio a 90-79 lead with 5:39 to play.
Most of Golden State's yellow-shirt wearing crowd of 19,596 silenced after Curry came off a curl and his left ankle — which he sprained in Game 2 in the first round against Denver but seemed to be back to full strength — landed awkwardly when he planted his feet to receive the ball. Curry limped around but stayed in the game, with nervous chants of "Curry! Curry!" breaking out.
The Warriors moved within five points on Harrison Barnes' pull-up jumper with 2:48 to play but never got closer. After starting 3-0 at home in the playoffs, Golden State fell short again in maybe the biggest basketball game in the Bay Area in decades.
The Warriors have not held a series lead beyond the first round since the 1975-76 season, when they went up 2-1 and 3-2 against Phoenix in the conference finals and lost. That also was last time the Warriors made the conference finals, though there were only three rounds in the playoffs at that time.
"We talked about it, even after winning Game 2, this is going to be a heavyweight championship fight," Warriors coach Mark Jackson said. "This is a different animal that we're going against. They have four future Hall of Famers. They're not going to lie down."
After trailing for 95 of 106 minutes in the first two games of the series, San Antonio had by far its best start behind its All-Star point guard.
Parker scored 13 points in the first quarter on 6-of-8 shooting, including a desperation bank shot after getting fouled by Green that gave the Spurs a 32-23 lead. The largest lead for San Antonio, which went ahead by 11 points throughout the second quarter, previously was five — all the way back in the second overtime of its Game 1 comeback win.
All-Star forward David Lee sparked Golden State with his first appearance in the series after tearing his right hip flexor in the first-round opener against Denver and originally deemed out for the season. Lee, held scoreless in a brief appearance in the Game 6 clincher over the Nuggets, instantly put back an offensive rebound while getting fouled by Tiago Splitter to start a three-point play that sent fans into a frenzy.
Lee finished with five points and two rebounds in three minutes.
Parker swished a pull-up jumper over Green to put the Spurs up 57-48 at halftime. He had 25 points on 11-for-14 shooting in the first half, while Curry (9 points) and Thompson (3 points) combined for 12 points.
The Warriors erased the deficit a little more than halfway through the third quarter when Curry's quick 3-pointer evened the score at 65-all. Bogut exited with 6:34 remaining in the period after picking up his fourth foul, and the Spurs surged ahead by 11 behind a quick burst from Manu Ginobili with Golden State's big man buried on the bench.


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Senate hopeful Cash to campaign in OC
A hopeful for the Republican nomination for the U-S Senate, Richard Cash, has announced two upcoming campaign appearances in Oconee County. Cash will join fellow Lindsey Graham challengers at a get together Monday night, sponsored by the Oconee Republican Party. He'll also meet and greet the public Saturday, April 19 at the Lighthouse Restaurant.


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After a great breakfast at La Iguana we walked up the steep hill into Santa Cruz. Checked out the old church witch had some really cool wood carved statues.
We then caught a very rough boat ride pack to Panajachel. The wind has been blowing like crazy since we got to Pana and today was no different. The boat hull was cracked right where we were sitting. You could see it moving up and down every time it smashed into the next wave. I can't imagine why, The boats where only about a zillion years old and being piloted by a 13 year old kid at warp speed. We got the bikes and headed out of Pana towards Antigua. the road we took was very small and bumpy. It was scenic though and we enjoyed being on the bikes again after a wonderful stay on Lake Atitca. Once we got to Antigua however the mood quickly changed. The city is a maze of one way streets and dead ends. With seemingly endless "church squares". We had a tip on a place to stay from the people at Iguana and they said it was "right near the town square" We spent about 2.5 hours riding to Antigua and about 1.5 hours riding around looking for THE town square. After many stops to ask directions, (a seemingly futile effort when they machine gun a bunch of Spanish at you) Timmy fell into the well again???? We would then head off to ride around some more. We then saw a beacon of light. The MOTO Cafe. We stopped in for a beer and had a chat with a cool British dude who owned it. He gave us some Ideas and we rode around more looking for a room. We did find one but not till after we both got frustrated. we check in and talked them into moving some furniture so we could put the bikes inside. We went out for a nice steak dinner and a few drinks.
Tomorrow we make tracks and start logging some miles again. Cheers!


text: 10/01/11 10/01/11 POPSUGAR Fashion Street Style Spring 2012 Paris Fashion Week Street Style: Day 3 50 Striking Street Style Looks From Day Three of Paris Fashion Week October 1, 2011 by Christina Pérez 3 Shares Chat with us on Facebook Messenger. Learn what's trending across POPSUGAR. View on One Page Photo 42 of 49 Start Slideshow ADVERTISEMENT () Share This Link Copy What's Your Reaction? Thanks for your reaction Don't forget to share this with your friends! ← Use Arrows Keys → 42 Paris Fashion Week Street Style Clotilde d'Urso Previous Next Susie LauIn The Looking GlassSpring 2012 Paris Fashion WeekTaylor Tomasi HillSpring 2012Anna Dello RussoGiovanna BattagliaMargherita MissoniStreet StyleParis Fashion WeekAnna Wintour


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CONTACT: Justin Hoyt, (518) 629-7898, email@example.com
FOR RELEASE: Immediate, Thursday, April 14, 2011
The Hudson Valley Community College baseball team (13-11) swept Schenectady County Community College (10-8) in a doubleheader, 1-0 and 10-8, on Thursday, April 14 at home.
Bryan Lajeunesse (Albany Academy/Watervliet) continued to dominate on the mound for the Vikings, pitching a complete game shutout with six strikeouts. Nick Papas (Maple Hill/Castleton) and Luke Burns (Delaware Academy/Bovina) each went 1-3 at the plate in the win.
In game two, Schenectady had a late rally, but was shut down by Josh Johnson (Tamarac/Wynantskill) in the seventh inning. Johnson got into trouble, loading the bases with one out and a slim two-run lead, but forced two consecutive ground outs to end the game. Tyler Young (Shaker/Latham) led the Vikings, going 4-4 with two doubles and two RBIs. Jake DePietro (Kingston/Kingston) recorded two doubles and three RBIs and Chris Massaroni (Voorheesville/Voorheesville) went 2-3 with a double and two RBIs.
The Vikings travel to Mohawk Valley Community College on Saturday, April 16, for a doubleheader starting at 1 p.m.


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Sunday, June 2, 2013 9:30 a.m. to 9:30 a.m.
Location: Trinitarian Congregational Parish of Castine, 68 Main Street, Castine, Maine
For more information: Rev. Tim Hall; (207) 326-9486; tcpoc.org
A service of holy communion will be held during Sunday morning worship, June 2 at the Trinitarian Congregational Parish of Castine. Rev. Tim Hall will preach at this ninth Sunday in Ordinary Time in a sermon entitled "Faith of an Outsider."Fellowship and refreshments follow the 9:30 am worship service and church school downstairs in the vestry of the "Main Street Church." As always, all are welcome to join in worship and sacrament.


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"America's Next Top Model" Ann Markley Is Back
Ann Markley is hardly an "America's Next Top Model" all-star. Even though she was completely stunning and the judges kept commenting on her incredible potential, she kind of sucked at the competition. Instead of rising to the top of the pack, she became a cranky nag, always picking at eventual winner Eva Pigford. But I am glad to see that, even though she wasn't picked for the "All-Star" season, Ann hasn't given up on modeling. Apparently, she's appeared in Shape magazine and has been in commercials for Travelocity and Wheat Thins. Oh, and here she is in a new commercial for Olay. We're so happy to see her again that we will kindly ignore the fact that the spot is for an over-the-lip hair removal cream. [ONTD]