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This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Synthesis of chromosomal DNA is initiated from multiple origins of replication in higher eukaryotes; however, little is known about these origins' structures. We isolated the origin-derived nascent DNAs from a human repair-deficient cell line by blocking the replication forks near the origins using two different origin-trapping methods (
DNA replication is a fundamental process for maintaining and transmitting genetic information to proliferating cells. In eukaryotes, genomic DNA replication starts bi-directionally from sites called "replication origins". Because the initiation step of DNA replication is crucial for regulation of cell proliferation, the structures of the origins and the proteins involved in their functions have been extensively studied. In
Over the past decades, several methods for origin mapping have been developed for identifying replication origins in eukaryotic chromosomes [
In this study, our intention was to systematically clone the BrdU-labeled nascent DNAs from arrested replication forks that had arrested near the origins and map the replication origins in the human genome at the nucleotide sequence level in order to elucidate the features of the origin sequences. To achieve this, first, we developed a novel origin-trapping method by forming UV-induced DNA lesions in a nucleotide excision repair-deficient human (GM8207) cell line. Replication fork progression was effectively blocked in the exponentially proliferating GM8207 cells by UV irradiation and Trioxsalen-mediated DNA crosslinking. We performed competitive PCR assays using the nascent DNAs from the treated cells and showed that DNAs from well-characterized
BrdU-labeled DNAs prepared by the two different methods were cloned for construction of genomic DNA libraries (
The nucleotide sequences from libraries I (85 kb) and II (208 kb) were compared with each other or with control sequences from the human genome to elucidate the features of the isolated putative origins. The results of this analysis indicated that the two libraries differed in their G+C composition and their replication-related motif contents. One hundred and seventeen and 172 putative origin loci from libraries I and II, respectively, were mapped to the human genome. In addition, the flanking sequences of the mapped origin loci were analyzed by comparison to 236 genomic control loci to examine the association of the origin loci with genes, DNase I hypersensitive sites, transcription start sites, CpG islands and an epigenetic marker of K27-acetylated histone H3 peaks.
Although our comprehensive study has been conducted on a relatively small scale, the origin-trapping methods reported here could be useful for studying DNA replication origins in a number of eukaryotes whose genomes have not been sequenced. That the methods require little or no specialist genome analysis tools (such as microarrays or next generation sequencers) should be a distinct advantage for many researchers.
In this study, we designed and used two different methods for isolating the nascent DNA molecules arising from DNA replication origins (
Scheme for trapping and isolating nascent DNAs from origins of replication in human cells. (
We first applied the UV-mediated origin-trapping method to the human SV40-transformed fibroblast cell line GM8207, which is deficient in nucleotide excision repair [
Inhibition of DNA synthesis in GM8207 cells after UV irradiation. (
We also compared the previously reported DNA crosslink-mediated origin-trapping method that uses Trioxsalen [
We initially examined the inhibitory effects of Trioxsalen treatment (
Inhibition of DNA synthesis in GM8207 cells by Trioxsalen treatment. (
To identify the early-initiating origins in the genome, we isolated nascent DNAs from GM8207 cells arrested in early S phase after double cell synchronization (
Analyses of DNA synthesis in GM8207 cells synchronized in early S phase. (
To isolate the BrdU-labeled DNA from the UV irradiated cells, we used alkaline sucrose density gradient centrifugation and immunoprecipitation with an anti-BrdU antibody, according to a previously reported procedure [
Purification of BrdU-labeled nascent DNAs.
|3H total count (cpm)
|14C total count (cpm)
|3H/14C ratio
|3H-labeled DNA abundance (fold)
|Total DNA
|Pooled DNA
|Immunopurified DNA
We also examined enrichment of the nascent DNAs from the known origins of replication using competitive PCR methodology [
Competitive PCR assay for assessing the relative abundance of DNA from
Abundance of the origin-derived DNAs in the DNA fractions purified from UV irradiated and crosslinked cells. The relative DNA abundances from the origin and non-origin loci compared with the β-globin_40k (
Based on the procedures described above, we have independently isolated and cloned BrdU-labeled nascent DNAs from UV (300 J/m2)-irradiated proliferating GM8207 cells and cells synchronized in early S phase. Because the quantity of the labeled nascent DNA was very small, and BrdU-labeled DNA is both heat- and photo-labile (e.g., heat denaturation at 95 °C for 5 min completely disrupted the BrdU-labeled DNAs), we avoided exposing the DNA samples to high temperatures and light during the cloning process. Nascent DNAs were cloned into plasmids via addition of poly dC tails at their 3' ends, oligo dG-primed complimentary strand synthesis, adaptor ligation, and PCR amplification (as described in the Experimental Section). Complementary strand synthesis by DNA polymerase was totally dependent upon both the poly dC tailing reaction and the oligo dG primer (data not shown). For DNA cloning from the UV-irradiated samples, we used T7 DNA polymerase for complementary strand synthesis at 37 °C; however, the resultant clones frequently contained non-specifically primed DNA inserts that were presumably caused by the repetitive sequences in the DNA samples. Therefore, despite the need to avoid using a high temperature reaction (65 °C for 4 min), we used Taq DNA polymerase for DNA synthesis for cloning the DNA samples from the synchronized cells. In addition, we implemented a size fractionation process for the amplified DNAs to remove the contamination derived from clones with short inserts. In this study, the two genomic libraries (I and II) were prepared from the UV-irradiated proliferating cells and the cells arrested in early S phase, respectively.
By subsequent sequencing of the DNA inserts in both directions, a large number of nucleotide sequences were obtained from each of the libraries, library I (133 sequences) and II (221 sequences) (
Characteristics of the sequenced clones from libraries I and II.
|Library I
|Library II
|DNA source
|UV-treated proliferating cells
|Synchronized cells in early S phase
|No. of determined sequences
|No. of independently sequenced inserts
|Total length of the sequences (bp)
|Mean length of the sequences (bp)
|640 ± 328
|941 ± 279
Note: Number of independently sequenced inserts: this denotes where two independently sequenced DNA inserts in a chimeric clone were counted as two, and two sequences from a single DNA insert (e.g., two sequences reads in both direction for a long-insert DNA) were counted as one. The mean length for each of the library sequences is shown with the associated standard deviation.
We next examined the abundance of each locus identified in the nascent DNAs using competitive PCR. This enabled us to examine if individual loci in the cloned DNAs were derived from an origin region. For this purpose, 107 sets of PCR primers were designed based on unique sequences in library II. After screening to obtain unique signals in both the PCR and Southern blot analyses using human genomic DNA, 27 sets of primers were finally obtained. Thereafter, out of 27 loci, 12 sets of competitive PCR primers were successfully generated (supplementary Table S1). The primer sets for three target loci (
Measurement of the relative abundances of the nascent DNAs from 12 library II loci using competitive PCR assays. The DNA abundance for each locus relative to that of XPD4-F10 is indicated. The locus name and the derived sequence ID (in parentheses) are shown. Details of the results appear in supplementary Table S4.
The nucleotide sequences of the DNA clones from the two libraries were characterized using bioinformatics to examine the features of their primary structures. We examined the G+C composition of a contiguously assembled sequence from each library, as well as the composition of the repeat sequences, and five replication-related sequences (ACS [
The G+C content and sequence compositions of repeat elements and replication-related motifs in libraries I and II. Supplementary Table S5 contains additional information. It should be noted that the human genome sequences used as controls contained no satellite elements. Bars represent the standard deviation of the mean.
The sequence compositions of the two libraries could be clearly distinguished by their G+C content as well as by the composition of some of the motifs and repeat sequences (e.g., MAR, AT-rich elements and Alu repeats). Few Alu sequences and a low G+C content in library II are features generally observed in the G-band region of chromosomes, and are consistent with the corresponding mean values of the six G-band loci (
The putative origin sequences from the libraries were further mapped to the human genome using BLAST/BLAT searches. One hundred and seventeen and 172 unique loci from 133 (library I) and 221 (library II) sequences were successfully assigned to their respective genomic locations on human chromosomes (supplementary Table S6 A,B).
Assignment of the putative replication origin loci from libraries I and II to human chromosomes. (
Next, we assigned each locus to a chromosome band. As shown in
Analyses of the flanking regions of the putative replication origin loci mapped to the human genome. The frequencies with which library I and II loci and 236 loci selected from the human genome (control human loci) mapped to G- or R-band regions (
We examined the flanking regions of the mapped loci in the human genome using the project data from ENCODE [
By contrast, TSSs, CpG islands, and K27-acetylated histone H3 peaks in the flanking regions of the 10, 20, and 50 kb windows, were similarly observed in both the putative origin loci and the control loci (
The human nucleotide excision repair-deficient cell line GM8207 (XP6BE[SV40]) from the XP-D female patient [
Exponentially growing GM8207 cells were cultured in 15-cm-diameter culture dishes with 20 mL of DMEM containing 10% dFCS (
DNA crosslinking with Trioxsalen and long-wave UV light was performed according to a previously reported procedure by Russev and Vassilev [
GM8207 cells were arrested in early S phase with replication inhibitors and labeled with BrdU to prepare nascent DNAs from the early-firing origins. Briefly, exponentially proliferating cells (≈50% confluent) in a 15-cm dish with 20 mL of growth medium were incubated with 1.25 μg/mL of aphidicolin at 37 °C for 12 h. The cells were released by removing the medium and successive washing with 20 mL of warm DMEM (twice), and cultured in 15 mL of warm growth medium containing 0.03 μg/mL colcemide for 12 h in the dark. Mitotic cells were collected into conical tubes and washed in 40 mL of warm DMEM twice as described in
BrdU-labeled DNAs in the fixed cells were purified by fractionation using alkaline sucrose density gradient centrifugation and successive immunoprecipitation with an anti-BrdU antibody. Cells were handled under dim light or in the dark. Cells fixed in ethanol were pelleted, suspended and washed in 1 mL of ice cold PBS(−) by centrifugation at 5,000 rpm for 2 min (4 °C). The cells were gently resuspended in 0.3 mL of TNE (10 mM Tris-HCl, pH 7.5, 0.1 M NaCl, 1 mM EDTA, pH 8.0) and incubated at 37 °C overnight in the presence of 1% SDS and 0.3 mg/mL proteinase K (Merck, Darmstadt, Germany). After gentle extraction by rotation with phenol-chloroform for 20 min and successive centrifugation at 12,000 rpm for 5 min, the DNA in the aqueous layer was precipitated with 0.25 M KCl and 2.5 volume of ethanol. After washing with 1 mL of 80% ethanol by centrifugation at 12,000 rpm for 10 min, the pellet was air-dried and dissolved in 50 μL of autoclaved distilled water at 4 °C for 5 h. The DNA solution was loaded onto 0.2 mL of alkaline lysis solution (0.5 M NaOH, 0.7 M NaCl, 10 mM EDTA, and 2% sodium
Purified BrdU-labeled single-stranded DNA was modified through addition of a poly dC tail at the 3'-end with TdT (Gibco BRL) and dCTP according to the manufacturer's instructions. Reactions were terminated by phenol-chloroform extraction and the DNA collected by ethanol precipitation. The complementary strand of the poly dC-tailed single-stranded DNA was synthesized with DNA polymerase and an oligo dG primer. Synthesis of single-stranded DNA from UV irradiated cells was performed with Sequenase™ (version 2, USB, Cleveland, OH, USA). After incubation with DNA and 50 pmoL of the 5'-phosphorylated oligo dG/I primer (5'-GGIIGGGIIGGGIIGGGIIGG-3') at 37 °C for 30 min in a 50 μL reaction mixture, 40 units of Sequenase and the recommended concentrations of dithiothreitol and dNTPs were added and the reaction incubated for 60 min. For nascent DNA from early S phase cells, rTaq DNA polymerase (Toyobo, Tokyo, Japan) was used for DNA synthesis. The 50 μL reaction containing the DNA sample, 10 units of rTaq polymerase, 125 pmoL of the 5'-phosphorylated oligo dG12–18 primer (Pharmacia, Uppsala, Sweden), and other components were incubated at 65 °C for 4 min, according to the manufacturer's instructions. DNA synthesis in each reaction was monitored in parallel reactions containing [32P]dCTP and successive 1.3% alkaline agarose gel electrophoresis and autoradiography. After phenol-chloroform extraction and ethanol precipitation, DNA ends were blunted using a DNA blunting kit (Takara Bio, Otsu, Shiga) and the 5' ends of the DNA were phosphorylated with T4 polynucleotide kinase (Toyobo or Gibco BRL), according to the manufacturer's instructions. DNA purified by phenol-chloroform extraction and ethanol precipitation was ligated to a DNA adaptor (UniAmp, Clontech, Mountain View, CA, USA) with T4 DNA ligase (Ligation high, Toyobo). Next, the adaptor-ligated DNAs were PCR amplified with an adaptor-specific primer.
Preparation and sequencing of plasmid DNA, followed by editing and assembly of the sequences were performed as described previously [
Competitive PCR was performed as a quantitative assay of the target sequences in the purified DNA fractions. Genomic DNA used as a control was prepared from confluent GM8207 cells in stationary phase. The procedure was conducted according to Diviacco
G+C content determination and prediction of replication-related sequence motifs (
The aim of this study was to isolate and clone nascent DNAs from replication forks that had arrested near origins of replication in the human genome. We characterized the effects of UV radiation and chemical crosslinking during DNA synthesis in human DNA repair-deficient cells and used UV radiation to clone the nascent DNAs from the origins in the exponentially proliferating cells (
We found that there are some potential issues to be resolved in our origin-trapping procedures. As shown in
The authors thank Masanori Naitou, Fumihiro Fujimori, Makoto Abe, and Kazuo Iida for invaluable support with the experiments, especially the competitive PCR assays. We are also grateful to Hiroko Hagiwara for initial contributions to the


text:
|Martin Josefsson <gandalf@xxxxxxxxxxxxxx>
|Re: [PATCH/RFC] Reduce call chain length in netfilter (was: Re: do_IRQ: stack overflow: 872..)
|"David S. Miller" <davem@xxxxxxxxxxxxx>
|Tue, 25 Jan 2005 22:05:58 -0800
|bdschuym@xxxxxxxxxx, shemminger@xxxxxxxx, dwmw2@xxxxxxxxxxxxx, ak@xxxxxxx, snort2004@xxxxxxx, bridge@xxxxxxxx, netdev@xxxxxxxxxxx, rusty@xxxxxxxxxxxxxxx
|<email@example.com> <email@example.com> <firstname.lastname@example.com> <firstname.lastname@example.com> <email@example.com> <email@example.com> <email@example.com> <firstname.lastname@example.com> <firstname.lastname@example.com> <firstname.lastname@example.com>
On Sun, 23 Jan 2005 17:08:29 +0100 Martin Josefsson <gandalf@xxxxxxxxxxxxxx> wrote: > I'm now running a kernel with this patch and everything seems to still > be working. > So unless someone else has something to comment I think this should be > applied. > The decrease in call-depth is important. I would like to see at least one ACK from the netfilter folks. Bart or Rusty, could you forward to patch to netfilter-devel for review? I have some other ideas about how bridging might be able to save some call chain depth... but I need to think about it some more before proposing or even trying to implement. (basically something akin to how we do route level packet output, via dst_output(), but instead we're doing this at ->hard_start_xmit() time)
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subset: commoncrawl


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It was in these conversations that the inhumanity of our health-care system truly hit home to me.
This is a level of inhumanity that frankly boggles my mind and makes me ill.
She opted to stay, believing her exit would be a victory for the authors of the inhumanity she dedicated her life to exposing.
And to the extent that we in America do not call our Israeli brothers and sisters on this inhumanity, we are complicit in it.
Since his videos exult in the killing of innocent civilians, any cross-examination would have emphasised his inhumanity.
The only way in which to describe these unfortunate subjects of man's inhumanity was as animated bladders of lard.
Cruelty and inhumanity in civil cases were as great as under the Inquisition.
We might add many other proofs of the inhumanity exercised towards Napoleon, were it necessary to our purpose.
They are to-day the most terrible proofs of man's inhumanity.
The inhumanity and craftiness of these propositions for the removal of the free people of color.
late 15c., from French inhumanité (14c.) or directly from Latin inhumanitatem (nominative inhumanitas) "inhuman conduct, savageness," noun of quality from inhumanus (see inhuman).
And Man, whose heav'n-erected face
The smiles of love adorn,--
Man's inhumanity to man
Makes countless thousands mourn!
[Robert Burns, "Man was Made to Mourn," 1784]


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I am a big proponent of gratitude.
Acknowledging that I am in favor of gratitude seems silly. Who isn't in favor of gratitude? In case you were wondering, I also like fresh air, holding sleeping babies in my arms, and freshly-made pie. Of course I am a proponent of gratitude! Gratitude is the basis of every spiritual practice. I have written, made videos, and talked about gratitude for years.
Science can demonstrate positive correlations between gratitude and academic performance. And there are studies that show a link between gratitude and higher levels of immunity.read more
Marching with thousands of joyful, passionate people at the Women's March in Seattle last weekend and seeing all the causes their signs supported – health care for all, diversity, respect and equal rights for all people, I realized the ultimate expression of all the things we were marching for would look, to me anyway, very much like the Culture of God; like the "Kingdom of Heaven" described by Jesus in the beatitudes. At the march in Seattle and marches around the world, people were intent on creating what they might call a better world, or a world of peace and justice. And if Jesus is right, if the excluded will be blessed by inclusion in the culture of God; if those who take action to make this world more like the culture of God will be blessed for their efforts, then with all due respect to Jesus and the original recorders of his words, I'd like to offer some beatitudes for the 21st century.read more
Listen to Boston Public Radio discussion of the painting and the outrage it has inspired with Reverends Irene Monroe and Emmett Price.read more
When artist Dana Schutz presented "Open Casket," an abstract painting of Emmett Till's open casket-the Chicagoan 14 year old African American male teen lynched in the Mississippi Delta in the summer of 1955- she could not have fathomed the conflagration that erupted.
The painting hangs at the Whitney Museum in New York City but under the daily watchful eye of protestors blocking its view they termed the "black death spectacle." Some protesters sent letters of grievances to the museum curators requesting the painting be taken down and others have flatly demanded the destruction of it.read more
Nicodemus has understandable cynicism about the realistic expectation that an adult can really make any substantial chance, any more than that an adult could enter his or her mother's womb a second time. This sermon takes that very real and practical question at face value. Beyond any concerns about life after death, can we, as adults, make a conscious decision to make substantive correction in the course of our lives? And if we can't, why would anyone bother with religious faith at all?read more
Remember, back in the olden days of late January 2017, when Sean Spicer and a host of other administration cronies promised that Trump was a steadfast ally of LGBT Americans, and that there were no anti-LGBT …read more
We got bells! We got arches! Well, really now . . . Arches 'n Bells is a skit, play, dialogue, monologue, reader's theater, and litany provider for churches and faith communities. Our writers focus on producing high-quality resources from a progressive, grace-centered angle. All the stuff is downloadable for you to print and use. Oh . . . and we're about fun too. Anybody going to the site should be able to see that. What's more is that these works are intergenerational—they work with kids, teens, adults, and various subsections of a faith community's demographics.read more
This is an especially difficult concept for us in the United States. Many of the people who helped form this country tried to solve their problems by simply moving away from people they had difficulties with. This method of dealing with problems is deeply embedded in the American psyche. When people get on your nerves, you can simply "go west" to get a little peace and quiet, a little piece of your own ground, and live with minimal interactions with neighbors. You don't have to learn to live with difficult people, rather you can just pick the people you want to be with. People first left the problems of Europe behind. When life here got too tense, many continued pioneering westward to get away from everyone. Part of the American Dream is the illusion that you can create and control the bubble you live in. Perhaps this method worked for a couple hundred years until the empty spaces ran out and we were stuck staring eyeball to eyeball with our neighbors, again. Still, the fantasy remains that the individual acting alone is the most advanced and enlightened form of human activity. Yet Christianity has always held that life is about "we" not about "me."read more
Question and Answer Question: Dear rB, You talk about loving and not being filled with hate. I remember this past summer you turned the racist, anti-Semitic graffiti outside your house into a teaching moment …read more
A recent University of Michigan study looked into the curious fact that most people do not change their prejudices when confronted with contrary facts but rather double down on their mistaken beliefs. It seems that John uses blindness as a metaphor for choosing not to see in the account of the man born blind. Both then and now courageous faith asks us to love truth enough to reject prejudice, propaganda, and political lies.read more
And to those who say God's work can only be done
by following certain rules, the rabbi says,
What if the compassion you show to your pets,
even the care you give to your cars, were given also
to your sisters and brothers on earth?
Today marks the first Sunday of Lent, a time of self-reflection and lament. It is often considered a season of darkness. Something I am all too familiar with. The season of Lent reminds me of walking a labyrinth. A labyrinth is a path that requires you to go in and come out the same way in which you entered. It is a journey towards the center, then back out again, into the world to which you came. You cannot skip the part you did not like, or go around a difficult feeling, you must return the exact way you entered. But, even though the path does not change, you have, and in this we find new life.read more
That's a gift of the Psalms, that praying them, we feel less alone. Those who wrote the psalms were imperfect, much like us. They didn't know everything, but they had feelings about everything. And, like us, they had multiple situations and events to have feelings about, some good, even great, some bad, even evil. They reflect the human range of experiences and emotions.read more
Inspired by the films of Terrence Malick and the poetry of Rainer Maria Rilke, the film tells the story of a young woman who is dealing with an intense case of spiritual doubt that has been building within her for quite some time. Plagued with nightmares, she arises early one morning, says a prayer by candlelight, and goes downstairs for a cup of tea and to quietly reflect on recent events. At this moment, we flashback to a worship service at her church. In a moment of frustration, she storms out, and is followed by a friend who confronts her and reminds her that doubt, in and of itself, is not a bad thing.read more
As we seek to move beyond the tired binaries of Left and Right, let's find ourselves in the fusion coalition that invites us to reconsider our prejudices and find common cause with our neighbors as we move forward together in doing justice, loving mercy, and walking humbly with our God.read more
During one of the airless afternoons I spent in St. Rita Sunday school, our teacher gave us the exercise of drawing the indulgences that we would give up for the upcoming Lenten season. Peering at the …read more
When I was a child, the word God was one of those words that adults either used in vain or in hushed tones. Outbursts of anger always included the word God. Strange and mysterious circumstances often resulted in the word God being used in hushed tones. I remember the very first movie I was ever taken to see. Bambi may have been a Disney movie, but when the shot that killed Bambi's mother rang out, as far as my mother was concerned, I broke one of the ten commandments when I shouted, "Oh my God." Mom warned me that when we got home there would be dire consequences for this offence which confused me to no end, because before the movie began, they did what they always did in back in the 1960's, they played, "God save the Queen" as we all stood to attention. God's name being sung out incurred no dire consequences.read more
There are differences within diversity. This issue scratches below the superficial veneer of diversity and is an eerie reminder of how minority groups are often pitted against one another.read more


text:
Information Staff Association Minutes November 3, 2004
Minutes taken by Elaine Bradshaw for Lisa Bowles
ISA Council Members and Officers Present: Grettie Bondy, Joan Goth, Sophia Morren, and Marko Sherbon
Members and Guests: Elaine Bradshaw, Barbara Perry, Annette Schweibert, and James D. White
After a tour of the Stephenson Research and Technology Center the meeting was called to order.
Reports: Elaine Bradshaw gave the TreasurerŐs report for Lisa Bowles.
Joan gave the Staff Senate report. The main topics of discussion were an update by Nick Kelly on the new health insurance plans and a review of the staff grievance policy. Because of new mass e-mailing policies, Staff Senate will be relying on the member group e-mail lists to disseminate information from now on. Joan reminded everyone that health insurance enrollment forms are due November 5.
Miscellaneous discussion: Joan announced that Jackie Farley is retiring. Barbara Perry will be taking contributions this week for a gift from Staff Senate.
Barbara brought a sample of the sweatshirt being sold by Staff Senate for the Staff Week fundraiser.
Staff Week: Joan opened discussion of ISAŐs part in Staff Week. Sophia reported that 98% of those surveyed thought that the art show should continue. Barbara added that the budget deadline is the first of December. Pro and cons of using the University Club were discussed, as were suggestions for expanding participation. Grettie moved that ISA host an art show for Staff Week 2005. Marko seconded and the motion passed. Sophia will chair the committee and Annette, Grettie, Marko and Joan will sit on the committee. Marko will send out an e-mail to solicit volunteers for vice-chair (chair for 2006).
December Meeting: The group discussed plans for a holiday party for the December meeting. Grettie brought up the need to decide on a community service project. Grettie will see what Debbie Copp has planned for Staff Senate and Joan, Grettie, and Sophia will pick a project for ISA. Joan will get a turkey and Marko will have it smoked by VanŐs. Grettie will look into the cost of sides. Elaine mentioned that Marilyn Nicely will be retiring in December. Joan will make up a certificate for Marilyn and have it framed to present to her at the December meeting.
The meeting adjourned at 1:00 p.m.


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Having just read the Tribune article, "Cleveland's charter school growth a cautionary tale for Chicago" (News, Oct. 24), I am reminded once again of the false narrative of "charters as saviors." It is instructive to consider two common claims of charter schools as Chicago Public Schools embarks on a plan to close many more public schools and open more charters.
Charters claim to have hundreds of students on waiting lists. Yet, WBEZ reported the day before the recent Chicago Teachers Union strike that Andrew Broy, President of the Illinois Network of Charter Schools estimated 1/3 of the city's charter schools have open seats. This was after their school year had begun.


text:
THREATS to Britain's forests were the central topic as a pests and diseases professional training day came to Redditch.
More than 60 workers who look after trees including some from as far afield as Oxford gathered at Redditch Town Hall on Thursday, May 1, for the latest news and advice from experts at the Forestry Commission and the Arboricultural Association.
The Midlands had been set to miss out on the national advice sessions when Redditch Council – which manages extensive woodlands in the town – invited the agencies to hold an extra event in the town.
The sessions in the council chamber covered how to prevent, spot and manage diseases such as ash dieback, acute oak decline and chestnut blight, as well as pests such as the oak processionary moth and long horn beetles.
A new national campaign to enable members of the public to help nip problems in the bud by recognising and reporting tree problems was also explained. The Woodland Trust's 'Observatree' scheme encourages people to become tree health champions, and provides a new Tree Alert website and smartphone app for directly reporting problems.
British biosecurity was another hot topic as experts warned that trees sold as 'native' may only be grown from native seeds, but actually brought on overseas and then imported, sometimes from heavily disease-affected nations.
The council's head of environmental services, Guy Revans, said: "We are delighted that the national agencies accepted our offer to come to Redditch. As a result, all our tree officers and tree surgeons are now armed with the latest information and advice as they go about looking after our woodlands. We couldn't have sent everyone to other courses so it's great that they came to us, and people came from all across the region too."


text:
Herndon VA (SPX) Jul 12, 2010
The U.S. Department of State has awarded a contract to Northrop Grumman Corporation to continue providing staff to conduct peacekeeping operations and humanitarianism assistance training for the African Contingency Operations Training and Assistance (ACOTA) program.
The contract has a potential value of $150 million over five years.
"The ACOTA program is to arguably the most successful engagement program that the U.S. State Department has had in Africa. The objective is to enable participating nations' militaries to develop their own trained unit capacity to provide peacekeeping support operations where required," said Mike Devlin, Northrop Grumman Technical Services International Program Director.
"We are most pleased that we have been again selected to continue successfully supporting the ACOTA mission."
"International collaboration is a crucial component to peacekeeping efforts," said Tom Vice, corporate vice president and president of Northrop Grumman Technical Services. "We've been supporting the ACOTA program for more than 11 years in 22 countries, and we embrace the opportunity to spend another 5 years supporting these African nations."
Northrop Grumman provides the staff and training to select African Nations through seminars, instruction, simulation-supported exercises and field training. Nations currently participating in the ACOTA program include Benin, Botswana, Burkina Faso, Burundi, Djibouti, Ethiopia, Gabon, Ghana, Kenya, Mali, Malawi, Mauritania, Mozambique, Nigeria, Senegal, Sierra Leone, South Africa, Rwanda, Tanzania, Togo, Uganda, Zambia.
Northrop Grumman has supported the African Crisis Response Initiative/African Contingency Operations Training and Assistance (ACRI/ACOTA) program since its inception in 1998 and designed the initial training materials and conducted computer-assisted peacekeeping exercises for African Partner Nations.
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Answer To What Ended The Last Ice Age May Be Blowing In The Winds
China seizes melamine-tainted milk powder: reports
China's AgBank offers room for improvement
Across the US, employer-sponsored gardens grow
Drought destroys millions of hectares of Russian crops: govt
Mexican sisters survive floods after four days in tree
WWF presses Romania for long-term flood measures
5.4 earthquake rattles southern California: USGS
Tropical depression forms in Gulf of Mexico
Northrop Grumman Wins African Training Contract
G. Bissau president warns army top brass, drug traffickers
Religious intolerance threatens Nigerian democracy: Jonathan
Chinese-built hospital risks collapse in Angola: state radio
Timor-Leste warms to Australia asylum idea
U.S. government challenges Ariz. law
Tibetan Adaptation To Altitude Took Less Than 3,000 Years
A Butterfly Effect In The Brain
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If you told me a Michelin-starred Chinese restaurant from London was planning to expand with a Las Vegas location, I'd be pretty excited. It would be big dining news, even on the noisy, celebrity chef-infested Strip. The thing is, when the impeccable new Hakkasan finally landed at MGM Grand, it was attached to the biggest nightlife experience ever—80,000 square feet and five stories and more than $100 million worth of crazy Vegas fun. Hakkasan had only ever been a restaurant, and now it was morphing into this mega-thing where super-famous DJs would pack in monster crowds. There's food?
As it turns out, there's nothing to compare to Hakkasan the restaurant. Chinese is not the trendiest cuisine these days, and there are far fewer great Chinese rooms (Bellagio's Jasmine, Wynn's Wing Lei, Palazzo's Zine) than stylish Japanese or pan-Asian restaurants on Las Vegas Boulevard. Hakkasan's environment matches the elegance of those places with a sleek, intimate, almost futuristic edge, creating a palpable feeling of exclusivity. Most tables are for four or more, nudging you into making an occasion out of your meal before heading upstairs to party, and the cavernous restaurant space is divided into latticework-framed "cages" that only build the intimacy. It may be the most distinct restaurant design on the Strip.
The food is flawless, and while things can get wildly expensive here, there are still plenty of well-priced, impressively executed dishes. The golden, mushroom-accented Hakka noodles ($18) are a great example of that, a simple but sublime bite.
What must you try? The steamed dim sum platter ($28) is more than just the best dim sum in the city, it absolutely shames all competitors. The scallop shumai and black pepper duck dumpling are my favorites from this perfect package. Duck is a big deal here, and there are lots of ways to get it. The crispy duck salad ($28), a seemingly simple platter of luscious meat and crisp greens with bits of pomegranate, pine nut and pomelo, is a signature dish, and one I wish I could eat every day.
Hakkasan serves the best possible versions of several standards. Mongolian beef is done with plenty of black pepper and supremely tender steak. Egg and scallion fried rice is another daily wish for me. And even the hot and sour soup with chicken is memorable. That doesn't mean there aren't more creative or exotic offerings. Roasted Chilean sea bass is lacquered in honey and a type of red char siu sauce, an unexpected but pleasant combination. The appetizer of seared scallop with fresh mango and chili is another slightly sweet surprise, and the spicy prawn dish is saturated in a Thai-ish, almond-laden curry sauce.
Most of us won't spend hundreds on whole abalone or braised lion head meatballs (relax, they're pork) with truffles and cabbage. But that's okay. Hakkasan is a surprisingly flexible experience with a long and diverse menu. Most of the attention might be directed upstairs, but this first-floor restaurant has raised the bar for Chinese food in Las Vegas.
Hakkasan MGM Grand, 891-7888. Monday-Wednesday, 5-11 p.m.; Thursday-Sunday, 5 p.m.-midnight.


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From our Bike Events CalendarJul27Thu1:00 pm Women and Gender in (Public) Tra... @ Free webinarWomen and Gender in (Public) Tra... @ Free webinarJul 27 @ 1:00 pm – 3:00 pmWebinar by SFU Continuing Studies (City Program) July 27, 1 pm PDT (4 pm EDT) Transportation infrastructure and services are often assumed to be "gender neutral", where projects and services are assumed to provide an equal benefit to men and … Continue reading →4:00 pm Queen Anne Greenways PLAY STREET! @ 1st Ave N & CrockettQueen Anne Greenways PLAY STREET! @ 1st Ave N & CrockettJul 27 @ 4:00 pm – Jul 27 @ 7:00 pmQueen Anne Greenways, a neighborhood group working to increase mobility, accessibility and flexibility in our streets, will be sponsoring its second annual Playstreet on Thursday, July 27, 2017 from 4:00 to 7:00 pm in 1st Avenue North at Crockett Street. … Continue reading →5:30 pm Move Seattle Levy Oversight Comm...Move Seattle Levy Oversight Comm...Jul 27 @ 5:30 pm – 7:30 pmCity Hall, Room L280 (All future dates: 4/27, 7/27, 10/26, and 11/30)FacebookTwitterRedditEmail5:30 pm South Lake Union Greenways SLUG ...South Lake Union Greenways SLUG ...Jul 27 @ 5:30 pm – 7:00 pmhttp://bit.ly/2uOFWHT So much going on in South Lake Union. Meetup with people who want the City to build infrastructure that let's us safely walk and bike in our neighborhood.FacebookTwitterRedditEmail6:00 pm Beacon Hill Safe Streets Monthly...Beacon Hill Safe Streets Monthly...Jul 27 @ 6:00 pm – 7:45 pmpicnic! This month BHSS is meeting at Jefferson Park just West of the Tennis Courts (where the picnic tables are)! Bring your favorite snacks to share, friends, family, and kids! Get updates on your neighborhood *and* entertain the kids! We'll … Continue reading →
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Replay and recap: City Council Position 8 transportation and housing forum
Your ballot will arrive in your mailbox within a week or so, and you only have until August 1 to figure out who you're going to vote for. Online registration and address changing already ended, but you have until July … Continue reading
Posted in news Tagged 2017 city council position 8 race, 2017 primary, hisam goueli, jon grant, mac mcgregor, sara nelson, sheley secrest, teresa mosqueda 1 Comment


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There may be more than 600 million metric tons of water ice sitting in craters at the moon's north pole. The discovery, made by an Indian spacecraft, could mean big things for human colonization of our nearest neighbor.
A radar experiment aboard India's Chandrayaan-1 lunar spacecraft has identified thick deposits of water ice near the Moon's north pole.
The US space agency's (Nasa) Mini-Sar experiment found more than 40 small craters containing water ice.
Illustration based on a NASA photo


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i usually drive 80 on a 65 mph speed limit.. i don't know.. when i'm at 65 i hate when cars tailgate me so i feel like i need to go faster..
but i don't go over 5 at a residential area and i never go over on a school zone or at least i try not to..
I drive at 55/60 mph. Waste of gas to speed.
I drive 10 mph over the speed limit. The exception is on streets in residential areas that are not very busy or are known for having a lot of pedestrian traffic, and near schools (regardless of the street).
I usually drive around the speed limit on the surface streets, give or take road conditions, weather, visibility, etc. If I'm going slower, I'll keep to the right-most lane. Speed limits are quite reasonable here in San Diego.
On the freeways, however... it really depends. I'll just say that I go, "with the flow of traffic." It's a thousand times safer to have traffic moving fast with a good flow than slow with people driving in random lanes. Example: Germany, despite having over half of its autobahn network with no posted speed limit, has a very low accidents/billion vehicle km rate. It's actually illegal to pass someone on the right over there in most circumstances. I hate when people drive slow in the left-hand lanes. SLOWER TRAFFIC KEEP RIGHT / KEEP RIGHT EXCEPT TO PASS / LEFT LANE FOR PASSING ONLY. Et cetera, et cetera.
If the freeway is wide open, and no-one is around, and the weather is perfect, I may be found pushing it up to 85, but that's damn near unheard of Usually I go about 70 in those cases (except where the limit goes up to 70, then I'll go 75). My car really wasn't built for speed (massive old-man car boatmobile thing), so that's fast enough for me most of the time.
I've almost never heard of a speeding ticket for someone going less than 10 mph over the speed limit, but that's pretty much standard here in San Diego. Keep it under 80 on the freeway and you should be okay.
One funny story, once I was going about 90 on a two-lane highway out in the countryside (it was a German car, not my massive old-man boatmobile thing) where the posted limit is 55. I saw the distinctive headlights of a Crown Vic creeping up behind me and I thought, "oh hell, I'm screwed." He pulled up beside me, gave a smile and tipped his hat, and flew by me. San Diego County Sheriff's Department.
[add status message]
Wrathfulram wrote:
I find it strange that people think its cute and funny to ignore speeding laws. You think your invincible in your lil peices of crap but news flash your not! and your endangering the lives around you. I just hope you die and not take anyone out with you. Inconsiderate assholes. Women your the worse when it comes to driving, fact is fact, 9 times out of10 when a car is speeding up to me even when im alittle over 60 its a woman.
More often for me it's I see both men and women drive poorly, but it's been more men that make the stupid decision of pulling out in front of me without looking first.
Lost :'(


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Men's NamesThese brain teasers rely on your ability to recognize groups of common attributes. For each of these puzzles you'll need to figure out why the words or letters are grouped as they are. Sometimes you will be asked to pick the odd-one-out or to place a new word into the correct group.
Each of the following names has a similar association.
Billy, Charley, Jack, John, Mickey, Tom
Which one of the following has the same type of association?
Andy, Dick, George, Harry, Paul, Teddy
HintEach name in the group can be associated with an animal in a common word or phrase.
AnswerTeddy is part of this group. Each name in the group is part of a common word or phrase involving animals.
Billy goat - a male goat
Charley horse - a muscle cramp
Jackrabbit - a type of hare
John Bull - a personification of England
Mickey Mouse - Walt Disney icon
Teddy bear - a stuffed toy
Tomcat - a male cat
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Limit values of archive_timeout, post_auth_delay, auth_delay.milliseconds. The previous definitions of these GUC variables allowed them to range up to INT_MAX, but in point of fact the underlying code would suffer overflows or other errors with large values. Reduce the maximum values to something that won't misbehave. There's no apparent value in working harder than this, since very large delays aren't sensible for any of these. (Note: the risk with archive_timeout is that if we're late checking the state, the timestamp difference it's being compared to might overflow. So we need some amount of slop; the choice of INT_MAX/2 is arbitrary.) Per followup investigation of bug #7670. Although this isn't a very significant fix, might as well back-patch. Branch ------ master Details ------- http://git.postgresql.org/pg/commitdiff/b6e3798f3aa2747db145f25e03a8d34f2e5ec8c8 Modified Files -------------- contrib/auth_delay/auth_delay.c | 2 +- src/backend/utils/misc/guc.c | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-)
Next: From: Tom Lane Date: 2012-11-18 22:16:04 Subject: pgsql: Limit values of archive_timeout, post_auth_delay,auth_delay.mil Previous: From: Tom Lane Date: 2012-11-18 21:17:00 Subject: pgsql: Fix syslogger to not fail when log_rotation_age exceeds 2^31mil


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For years, it has been one of the unwavering tenets, arguably the most critical axiom, on which NFL defenses have operated: Stop the run, and you will likely win.
In this the year of the pass, however, the truth in that statement has been hard to find.
Of the top 10 teams in the league versus the run, only half have winning records. The ratio isn't really much better -- six clubs on the plus-side of the ledger -- for the top 10 defenses against the pass.
It might seem like a conundrum, but players around the NFL just figure you've got to play solid enough defense, period, to win.
No edge to stuffing the run or the defending against the pass. Keep people out of the end zone, author a few big plays, and try to fend off the offensive onslaught that has marked the outset of the year.
And count on the offense -- the bottom three teams in the league in overall defense (Buffalo, Oakland, New England) are a cumulative 11-4 -- to score enough points.
The formula for success has changed a bit, at least through five weeks of the 2011 season.
"It used to always start with (stopping) the run," acknowledged Green Bay cornerback Charles Woodson. "That's still essential. I mean, we won (Sunday) night when we tightened up against the run after the first two series, but that's not the only thing anymore. You've got to be sound in everything you do.
"But the way the game is now, there's only so much you can do."
It's notable that three of the four lowest-ranked defenses against the pass feature winning records after five weeks of the season.
The Packers, who are ranked 30th versus the pass, are one of only two undefeated franchises remaining. The Patriots are last in the NFL against the pass, but are 4-1. That would seem to be a recipe for disaster with offenses throwing the ball so much, but it hasn't been.
And a big reason is that offenses have dominated.
Defense was always about the complete game, not just stopping the run. But as Atlanta middle linebacker Curtis Lofton noted a day after his team's Sunday loss to the Packers: "It's probably a little bit 'completer' now. ... And tougher."
Obviously, with rules changes, and the obsessive protection of the quarterback, the game in large part has swung toward the offense.
Eight of the top 10 offensive teams in the league own non-losing records at this juncture of the season. The Philadelphia Eagles rank No. 3 offensively and the Carolina Panthers are fifth, and both have 1-4 record, but they seem to be aberrations.
"The worm has definitely turned," allowed a standout NFC linebacker who spoke only for non-attribution. "You'd like to think it's still about playing great defense, but how many teams can do that anymore? Things seem to have evolved away from the defense. That's a hard pill to swallow, but it's true."


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I`m using frodo player for a short time now and already it seems the best for my carputer, few questions though. How can i set up the dvd player (subtitels and such) and secondly how come my subtitles are shaking a few pixels, it`s almost unreadable and they are green, can i set it to white or something?
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Reynolds Boughton RB44
The 4x4 Reynolds Boughton RB44 looks for all the world like a Ford Transit van (of the 1970s) on performance enhancing drugs. In many ways that's just what it is because the standard engines and transmissions are Ford units.
The RB44 was developed in the late 1970s with an eye to military contracts
to take over medium four wheel drive duties
as carried out by the ageing 1-ton and
Land Rovers,
such as towing the 105mm light gun and other equipment cross-country.
You do see a few in the hands of local authorities and
public utilities, this one being the basis of
an emergency rescue vehicle.
The RB44 has a conventional ladder chassis, live axles, and leaf springs over those axles.
Drive passes from the gearbox by a short propeller shaft
to Reynold Broughton's own transfer case.
This uses a full-time four wheel drive system with diff' lock.
The transfer case has two power take offs (PTO) for
driving auxiliary equipment.
The interior of the RB44 is just like any conventional (fairly-) modern van apart for the extra controls for four wheel drive and selecting the auxiliary equipment.
It is quite a substantial vehicle,
particularly on its 9.00x16 (34") tyres,
and the view forwards is very good.
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|Follow PSU Women's Soccer:
|Follow @PennStateWSOC
BLOG: Two Worlds Collide in Unique Penn State Practice
Nittany Lion Captains Selected in 2016 NWSL College Draft
Britt Eckerstrom, Mallory Weber join Western New York Flash; Raquel Rodriguez selected No. 2 by Sky Blue FC
Coaching Staff Separates Nittany Lions From the Pack
Lions staff an invaluable asset to head coach Erica Walsh
FEATURE: A Unique Journey to Nittany Lion Women's Soccer
Nittany Lions open Big Ten Tournament on Wednesday
Women's Soccer Downs Villanova in Exhibition, 3-1
Freshmen score two of the three Penn State goals
Ann Cook enters her 11th season with the Nittany Lions in 2017. This will also be the 11th season that Cook will be coaching under former teammate and friend Erica (Walsh) Dambach. The two combined for many wins on the field at William & Mary and have already shown their prowess on the sidelines together, as they led the Nittany Lions to the program's first NCAA title in 2015, two College Cup appearances (2012 & 2015), nine Big Ten titles (2007-12 & 2014-16) and 10 NCAA Tournament appearances (2007-16).
As the associate head coach, one of Cook's main duties is the Nittany Lion offensive scheme.
Since Cook has been with the Blue & White, Penn State has recorded four of the top-10 single-season offenses in program history. During the 2011 season, Penn State recorded 226 points on the year, which ranks second in program history - seven shy of the program record 233 points set back in 2001.
En route to the NCAA title and a pair of Big Ten titles in 2015, the Nittany Lions scored 64 goals and 66 assists. The 64 goals and 66 assists rank seventh and third in program history, respectively.
Under the guidance of Cook, four Nittany Lions have scored 100 or more points over their career. Maya Hayes finished her Nittany Lion career with 163 points (71g, 21a), which ranks third-best in school history. Most recently, Mallory Weber became the 10th Nittany Lion to eclipse the century mark in points during the 2015 season. Weber ended her career at Penn State in 10th with 101 points on 36 goals and 29 assists.
For the eighth consecutive year, in 2014, Cook traveled to Central America with an organization called Soccer Without Borders. The organization focuses on using soccer as a tool for youth development around the world. The exchange team has coached nearly 800 girls, trained more than 45 coaches and, in 2013, played two friendly matches against the Nicaraguan Women's National Team.
Prior to PSU, Cook was an assistant coach at Nebraska for two seasons. Cook was responsible for numerous tasks while at Nebraska including individual and team training, recruiting, video analysis and scheduling. She also worked closely as the staff's liaison to the Huskers' athletic support staff.
During her tenure at Missouri State from 2004-05, she handled training, recruiting and team travel duties as well as supervising the strength and conditioning program. The head coach at Drury University from 1999-2000, her squad ranked No. 5 in the NCAA Division-II poll among new programs in 1999.
A three-time All-American, Cook played for William & Mary from 1993-97, medically redshirting during the 1996 campaign. In the mix for the Hermann Trophy as well as the M.A.C. Player of the Year Award in both 1995 and 1997, she was the 1997 CAA Conference Player of the Year and CAA Conference Tournament MVP. The Tribe made appearances in the NCAA Tournament quarterfinals two of Cook's four seasons, grabbing a bid for the Tournament each year and concluding the seasons ranked in the top five.
Active with the U.S. National Team programs, she was a member of the U.S. Under-20 team from 1994-95 and played with the full national team in 1998.
Professionally, Cook was a fourth-round draft pick (25th overall in the global draft) by the Bay Area CyberRays in 2000. A member of the team that won the WUSA's inaugural season championship in 2001, she was traded in December of that year to the Washington Freedom, which eventually finished as the 2002 league runners-up. Cook picked up coaching full time when the WUSA folded after the 2003 season.
As a member of the W-League's Chicago Cobras from 1998-2000, she was the championship MVP in 2000 after helping the team to a league championship. In 1999, her team finished as the league runners-up.


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Michael J. Fox reunited with his 'Family Ties' co-stars when receiving the Fan Favorite Award from comedian Ben Stiller.
Celebrities from television, film and music came together to celebrate 2011 TV Land Awards which was held for the first time in New York on Sunday, April 10. The ceremony was filled with tributes to iconic shows such as "Family Ties", "The Cosby Show", "Welcome Back, Kotter" and "The Facts of Life".
To mark the change of venue, Liza Minnelli performed "New York New York" as the opening. The event went on honoring Regis Philbin with Legend Award, Daryl Hall and John Oates with Music Icon Award, and the aforementioned TV shows. Jane Lynch, Barbara Walters, Jane Leeves, and Ben Stiller were among the presenters.
Stevie Wonder performed his hit "Higher Ground", followed by Billy Vera who sang the songs from "Family Ties". In the middle of the show, a parody of "The King's Speech" was slipped along with a tribute for television housewives by the cast of "The Real Housewives of New York City".
The show will be aired in its entirety on Sunday, April 17 at 9 P.M. ET/PT.


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Raiders of the Lost Ark (1981)
. In the uncut conversation between Indy and Marion at the Raven Bar, Marion explains the cause of Abner's death and tells Indy about the difficult last two years of her life. After having arranged the following day's appointment for the delivery of the medallion, Indy turns to leave, only to return under Marion's urge; she grabs his jacket and pulls him close for a kiss. The whole Raven Bar scene was cut because it was considered too long. The kissing part can be seen in The Making of Raiders of the Lost Ark documentary. The scene appears in a script draft, the novelization and the Marvel Comics movie adaptation.
. A plot element involving the Ark of the Covenant was cut from the film and is only hinted at during the finale when the Ark is opened. Basically, there were 2 rules about the Ark not mentioned in the final cut of the film:
1. If you touch the Ark, you die. 2. If you look at the Ark when it is opened, you die. This is first explained in additional dialogue for the scene when Indy and Sallah visit Imam. Before translating the writings on the headpiece that give the height of the Staff of Ra, Imam warns Indy not to touch the Ark or look at it when it is opened. The next scene involving this Ark subplot is when Sallah and Indy remove the Ark from the Well of the Souls. When Sallah first sees it he reaches out to touch it. Indy stops him before he does and reminds him of Imam's warning. Then they insert long poles through each side of the Ark to lift it out of its crypt. Notice that nobody ever touches the Ark throughout the rest of the film until the finale.
. While Indiana Jones is in the Map Room trying to find the exact location of the Well of the Souls, two German soldiers approach Sallah and order him to help them remove their truck that had been stuck in the sand. In the process Sallah loses the rope with which he was supposed to help Indy get out of the Map Room. In a continuing scene we see Sallah entering a German tent looking for something to substitute his missing rope and awhile later he comes up with a "rope" made of bed sheets and a German flag. What we don't see is Sallah in a state of panic wandering in the Nazi camp at breakfast time. A group of German soldiers ask him to serve them water from a marmite. Sallah spills the content of the marmite on the German officers' uniforms and leaves promising to return with fresh water. The scene called for smoke in the background but unfortunately the tires used to produce the smoke were too many, thus making the scene too dark. Steven Spielberg opted to cut this scene instead of spending half a day shooting it again.
. With Indy and Marion left to suffocate in the Well of the Souls the Germans decide that Sallah should be executed. A young German soldier was supposed to carry out the dirty job but things changed when the later started having second thoughts. A German youth vacationing in Tunisia had been hired for the part of the young soldier. The funny thing is that this inexperienced young man managed to express brilliantly the moral dilemma of a young soldier forced to decide whether or not to kill a harmless stranger; this is the business of foreign wars, and this unknown German boy was able to convey it as well as any actor. The result was beyond description, with this German student playing an intensely moving and emotional scene, making David Wisnievitz and Karen Allen call it the greatest moment since the film began. Spielberg said the bitter truth was that brilliant and memorable though the scene was, it would probably end up on the cutting-room floor because it was just too long. So it did.
. There was a small cut during the scene where Indy and Marion escape from the Well of Souls. When Indy pushes the large stone brick out of the room filled with mummy's, there is an Arab guard standing outside. When the Arab and Indy come face to face, Indy simply knocks him unconscious. Evidence of this scene still remains in the film. As Indy steps out of the chamber, he pauses and looks off to the left side of the screen. In the following shot, when Indy and Marion run from the building to the German plane, that Arab is visible lying on the ground by the building.
. Indy surviving the submarine journey by lashing himself to the periscope with his whip. In the final film, the plot hole goes largely unnoticed. Most people seem to assume either that Indy snuck inside the sub or that it stayed on the ocean surface for the duration of the trip. This cut scene did appear in the Marvel Comics adaptation. The few pictures that we have found prove that they indeed film part of this scene.


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Recently Watched
Most Popular Review
This takes drama to a whole new level. Awesome cast and wonderful melodramatic story. The actors captivated my heart with their tears.
It's a really cute drama. Funny from beginning to end.
Well it stated kinda cute, then it was weird, but I can't believe I watch all episodes with the hope that she was gonna get a make over and get rid of her ugly nappy hair but that never happen.


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by Staff Writers
Beijing (AFP) Sept 02, 2013
China on Monday lowered its figure for economic growth for last year to 7.7 percent from 7.8 percent, the National Bureau of Statistics said, in an unexpected downgrade for the key number.
The world's second-largest economy has long been looked to as a potential driver of global recovery, but has put in a mixed performance in recent months.
The new figure posted on the National Bureau of Statistics website remains the lowest for gross domestic product growth since 1999, when it expanded 7.6 percent.
China's GDP stood at 51.9 trillion yuan ($8.5 trillion) for 2012, the NBS added.
The country officially overtook Japan as the world's second-largest economy in 2010 and the growth figure is a key statistic for global investors, businesses and institutions.
Like other economies, China regularly revises its annual GDP figures although it usually does so in an upward direction.
But now its leaders are looking to steer it towards a hoped-for soft landing after years of high-speed expansion.
The statistics bureau cited "more comprehensive and reliable fundamental" information for the change.
The revision was "preliminary", it added, and the figure could be altered again as more data was obtained.
The 7.7 percent result remains above the government's economic growth target for last year of 7.5 percent.
Beijing normally announces a conservative growth target and generally exceeds it.
The target for this year is also 7.5 percent. But after an acceleration to 7.9 percent in the final three months of 2012, growth slowed to 7.7 percent in the January-March period and 7.5 percent in the second quarter.
Growth in the first six months of the year came in at 7.6 percent, the NBS said in July, calling the performance "generally stable" and within expectations.
Despite two straight quarters of slowing growth, recent data for the current third quarter have been surprisingly solid so far, causing some economists to believe that it may be poised to stem the slide.
The 2012 figure compares with expansions of 9.3 percent in 2011 and 10.4 percent in 2010 and underscores a slowing trend after years of double-digit growth.
China's leaders say they want to retool the country's economic model away from a reliance on big ticket government-led investments and make private spending the key driver for what they hope will be expansions that are sustainable.
President Xi Jinping said in April that China's days of "ultra-high speed" growth are probably over, but he said the country can "sustain a relatively high speed of economic growth".
The reliability of China's statistics often prove vexing for economists who follow the country.
Even new premier Li Keqiang has expressed doubts, telling the US ambassador to China in 2007 when he was a top provincial official that some Chinese data were "man-made" and thus unreliable, according to leaked US diplomatic cables.
Li said he focused on only three figures -- electricity consumption, rail cargo volume, and the amount of loans issued -- when evaluating the provincial economy, according to a confidential memo released by the WikiLeaks website in late 2010.
"All other figures, especially GDP statistics, are 'for reference only,' he said smiling," according to the cable.
China manufacturing rebounds to 50.1 in August: HSBC
The British banking giant's purchasing managers' index (PMI) for last month improved from an 11-month low of 47.7 in July and came after three months of contraction, HSBC said in a statement.
It was unchanged from the bank's preliminary reading released last month.
The index tracks manufacturing activity in China's factories and workshops and is a closely watched gauge of the health of the world's second-largest economy. A reading below 50 indicates contraction, while anything above signals expansion.
The government's official PMI for August, released by the National Bureau of Statistics on Sunday, came in at a 16-month high of 51.0.
The August rebound suggested that growth in the sector has started to stabilise thanks to a modest improvement in new orders and output, said HSBC economist Qu Hongbin in the statement.
"This was mainly driven by the initial filtering through of recent stimulus measures and companies' restocking activities," he said.
"We expect some upside surprises to China's growth in the coming months."
Authorities have so far been reluctant to introduce large-scale stimulus measures, but in late July did announce some steps to boost growth, such as reducing taxes on small companies and encouraging railway development.
The first half of this year saw analyst concerns about China's economy mount after an expected rebound from growth of 7.8 percent last year -- the worst performance in 13 years -- failed to materialise.
Growth dipped from 7.9 percent in the final three months of last year to 7.7 percent in the January-March period and 7.5 percent in the second quarter.
China's leaders say they aim to move the economy away from dependency on big ticket investment and instead want consumer demand to become the key growth engine.
Authorities are targeting 2013 growth of 7.5 percent -- the same as the objective set last year.
They see annual growth in the seven percent range as being more sustainable for the future as China's economy matures. As recently as 2011 GDP grew 9.3 percent, and it expanded 10.4 percent in 2010.
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Bluehost Web Hosting Help
Wordpress Plugins to increase Performance.
My Wordpress site is slow or seems down.
Occasionally when your site gets a large number of simultaneous visitors the site could appear down due to the overwhelming number of php processes running on the server.
The best way to combat this is to install a caching plugin Caching plugins will create static html files with identical information and allows simple html pages to be served faster with less load on the server.
Two very popular caching plugins are W3 Total Cache, and WP Super Cache.1. Please log into your personal install of Wordpress. 2. Click on the Plugin Tabs Add New. 3. Then type the plugin name WP Super Cache or W3 Total. 4. Click on Install chosen Plugin and then Activate the Plugin. 5. Each plugin will need to be configured. For WP Super Cache visit Settings WP Super Cache page, and turn it on. 6. After you turn WP Super Cache please scroll down on the WP Super Cache option page screen until you see the Mod Rewrite Rules Section. 7. Then scroll down a little bit more and click on Update Now.
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Lifetime to Premiere ABBY'S ULTIMATE DANCE COMPETITION, 10/9
Lifetime's new reality series ABBY'S ULTIMATE DANCE COMPETITION is set to premiere on Tuesday, October 9, at 9 pm ET/PT. Headlined by Dance Moms' Abby Lee Miller, the competition series will feature 12 of the country's most talented girl and boy dancers competing for a $100,000 cash prize and a scholarship to the Young Dancer Program at the Joffrey Ballet School in New York. Joining Miller at the judges table will be Robin Antin, founder of the pop group sensation Pussycat Dolls, and dancer and cutting-edge celebrity choreographer Richard Jackson (Lady Gaga's "Marry the Night"). Kevin Manno (MTV's The Seven) will host.
Leading into the series' premiere at 8 pm PT/ET, Lifetime will air the Abby's Ultimate Dance Competition Casting Special to unveil the competition's dancers, their moms and judges. Lifetime has ordered 10 episodes of Abby's Ultimate Dance Competition, which will lead-in to the premiere of the network's all-new hidden-camera comedy show Prank My Mom, hosted by Vivica A. Fox.
Abby's Ultimate Dance Competition will begin by following a dozen aspiring dancers -- ages six through 13 - who will learn new routines and compete in a number of challenges designed to test their skills when they perform before Miller, Antin and Jackson, as well as a live audience, at the historic Los Angeles Theatre. Along the way, each competitor will be accompanied and coached by his or her own mother, who will make critical training, costume, music and choreographic decisions for their child while also navigating the precarious waters of the other highly competitive parents. As Miller, Antin and Jackson must make the sometimes easy and other times difficult decision to eliminate one dancer each week, the intense on stage competition and stakes between dancers and mothers increase throughout the season - culminating with a finale performance that assuredly will change the winning dancer's life forever.
The current season of Dance Moms, featuring Miller, will air its 90-minute season finale next Tuesday, September 11, and will be followed by two all-new cast reunion specials premiering on September 18 and September 25 at 9 pm ET/PT.


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USA-Spec PA12-BMW beats Dice Silverline
View Single Post
Registered User
Join Date: Aug 2008
Location: Brampton, Ontario, Canada
Posts: 765
My Ride: 2003 325i
View My Garage
Originally Posted by
Hey Steve, I know this is an old thread, but I just installed my PA12 and love it so far. Sounds quality is great and the unit is quick. I have a 2001 330ci w/HK and I have to press the "m" between left and right seek buttons to skip 10 tracks. When in random mode, it does not do the fast forward feature you described as earlier. I tried just holding seek too....no luck. Also Iphone 4.0.1 only seems to work in Direct mode. Anyone else have luck with fast forwarding on new PA12s? The user manual doesn't even mention the random function.
-PS nice iron ring in the video, just got mine recently from McMaster
Hi, glad to hear that the install went well & that you like the sound.
You have a combination the I have no direct exposure to - a Business CD HU pre-2003 and a iPhone with os4.
USA-Spec has updated their manual and they explicitly say that they support os4. I don't know where you bought your unit, but from your description of hitting the 'm' key to skip, it sounds like a recent version. They have definitely changed the behaviour of some of the functions. Please see section 3.1.2 of the current manual:
They also have this note:
Note: By setting DIP # 3 to OFF position, radio button will be back to
single-press operation mode but text display feature will be disabled
It sounds like you either have dip switch 2 or 3 in the off position. The new manual really doesn't cover the function of dip sw 3 that well & doesn't talk about the function of dip sw 4 at all.
Can I ask how you have them set? With 2 through 4 in the on position, playlist mode should work as well.
Another thing you may want to try is an older iPod, but the easiest way to know for sure is to read your firmware version of the sticker on the PA12-BMW and email their tech support and ask them about it. They are pretty quick to answer.
Please post your progress to keep others informed.
Congrads on the ring
I'm an old guy - Ryerson '93 - the second year that they were allowed to hand out degrees. I grew up in Chippawa before going to school in 89. Inlaws are still there. My nephew also just finished his first year of engineering @ Mac. Nice school.
USA-Spec iPod Interface Comparison
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2009 750i vibration problem
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Location: Calgary
Join Date: Jan 2010
Posts: 18
Mein Auto: 2008 BMW 750i
View My Garage
Originally Posted by
Thanks again LovinM6, your words actually gave me a smile when you credit me with great patience. To me that is a great compliment.
Thanks Ian_L...that sounds like a plan.
I got some shocking stuff to share but it might be kinda of longer post so I will share very soon.
To everyone reading...I just want to say thank you for all your encouragement and great input. Kinda feel sorry that this post is of a negative nature and not of a joyful one as many of the posts are. So..I sincerely thank YOU.
Bcool absolutely no reason to be sorry. BMW should be sorry... NOT you at any stretch of the imagination. Seems like that most of us especialy myself are really appreciative that you are sharing this unfortunate experience.
As Asaseaben wisely stated, no one should have to pay for a premium car as it sits in the shop with no fault of their own. I really starting to wonder why on earth BMW will not own up or man up also. Is this another denial/conspiracy like Toyota? Hmmmm. Sure smells like it.
Obviously lot of happy owners with flawless cars out there but dang this is a nightmare indeed. 4 months in the shop now? PLEASE hang in there and please give us that update. I also concur BMW 100% should give you a new car and full compensation of the crap you have to go thru!
Hope you enjoy the gold medal game as I am (writing this during 2nd period intermission): Canada 2 USA 1 end of 2nd!!
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TheShipsList-L ArchivesArchiver > TheShipsList > 2004-08 > 1091677429
Subject: Ship Phoenix - Captain John Sparrier, 1700's
Date: Wed, 4 Aug 2004 23:43:49 EDT
Hi Listers,
I would appreciate it if someone can direct me to the right source. I just
found out that my John Hartman and his family arrived in Philadelphia on the
ship Phoenix - Captain John Sparrier was the commander.The Hartman family was
living in Reutlengen, Wurtenberg, Germany, about 1700.
Would it be possible to find a record of the ship Phoenix? What would have
been the port of departure for people from Wurtenburg, Germany? Would there be
any sketches of ships in that time period?
Thank you,
Mary Boudreau
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|Ship Phoenix - Captain John Sparrier, 1700's by


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I'm still reeling from Atonement. Charlotte Brontë destroyed me with Villette. And, I'm glad the Huffington Post agrees that Liane Moriarty's bestseller ends with a, well, twist because I was bowled over.
Thing is, I hate surprises. Really, I do. I actually break out in hives at the thought of a surprise birthday party. Whether the surprise is good or bad is irrelevant. I don't like to be there when it's happening. My peripatetic childhood, which involved periodically arriving in a new place whose culture (and even language) I didn't understand made me wary of the unexpected. That, combined with my type-A, OCD nature makes me dread anything I can't see coming from a good distance. (I will be taking all of this up in therapy some day, do not fear.)
As a result, I am one of those rare people who also appreciates a certain amount of predictability in my reading. Rather than finding a book whose ending I can intuit or guess at a waste of time, I enjoy being able to focus my attention on the author's prose, character development, and attention to detail. I like category fiction because it generally follows a formula and it's the skill of the author at things other than surprising us that tends to set these works apart.
So, of course, it irks me no end to admit that some of my most memorable reading experiences have involved not just a surprise ending but a shocking one. My initial response is usually rage and confusion, followed, after a while, by admiration at the author's ability to yank the rug so forcefully out from under me. It's so hard to pull off, but when it's done right, it tends to make the narrative it closes unforgettable—especially when the finale seems organic and not gimmicky. I hate surprises but I tend to end up loving books that surprise me.
What are your favorite surprise endings? And why?


text:
President Obama broke the ice with Vice President Xi Jinping of China today by telling a little joke about one of his favorite states: Iowa.
When Xi mentioned he was going to visit the Hawkeye State, Obama noted that he and two other high-ranking U.S. officials -- Vice President Biden and Secretary of State Hillary Rodham Clinton -- "all know Iowa very well," said White House spokesman Jay Carney.
Obama, Clinton and Biden all competed for the 2008 presidential nomination, starting with the Iowa caucuses that Obama won -- a pivotal victory on his way to the White House.
Carney said that of the three U.S. leaders, "I would say that it's fair to say that the president probably has the fondest memories of Iowa."
Xi also invited the president and first lady Michelle Obama to China, Carney said.
The response may depend on whether Obama wins re-election this year.
Here's a full readout from Carney on the Obama-Xi meeting:
Let me take a step back, if I might, to give you a sense of this visit. It is a reciprocal visit. As you know, Vice President Biden visited the People's Republic of China last year. And Vice President Xi of the People's Republic is now here being hosted by Vice President Biden. He met, as you know, earlier today with President Obama and they had a good meeting.
It was quite a long meeting, in fact, in part because of the full range of issues that our two nations have to discuss. It is an important bilateral relationship, and it is one where we speak candidly about the concerns we have, whether it's issues of trade or currency, or, as you raised, matters of national security or foreign policy, as in Syria.
So I don't have a specific point-by-point readout yet of the president's meeting with Vice President Xi, but you can be sure that they discussed the full range of issues, that Vice President Xi will have those discussions not just with the president, but with Vice President Biden, with Secretary of State Clinton and -- and others.
I can tell you that, as I mentioned, the meeting ran long. It lasted from 11:25 a.m. until 10 minutes to 1. The president said afterwards that the reason he was taking the extra time was because of the importance of the relationship and cooperation in dealing with the range of challenges that these two -- our two countries face together.
The meeting began on a lighter note when Vice President Xi said he was going to Iowa on Wednesday, and the president replied that he, Vice President Biden and Secretary of State Clinton all know Iowa very well.
I would say -- and if I may -- of those three, I would say that it's fair to say that the president probably has the fondest memories of Iowa.
At the end of the meeting, Vice President Xi invited President Obama and the first lady to China. ... Stay tuned.
David's journalism career spans three decades, including coverage of five presidential elections, the Oklahoma City bombing, the 2000 Florida presidential recount and the 9/11 terrorist attacks. He has covered the White House for USA TODAY since 2005. His interests include history, politics, books, movies and college football -- not necessarily in that order. More about David
Follow the Oval on Twitter: @The Oval, @djusatoday.
Also contributing to the Oval: Richard Wolf @richardjwolf; Aamer Madhani @AamerISmad


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Thu Nov 8, 2012, 12:33 PM
JRLeft (7,010 posts)
Can a Texas Duer give me the break down of dems in the state, and how many non-voters are in the
8 replies, 966 views
Always highlight: 10 newest replies | Replies posted after I mark a forum
Replies to this discussion thread
|8 replies
Can a Texas Duer give me the break down of dems in the state, and how many non-voters are in the (Original post)
|Nov 2012
|Nov 2012
|Nov 2012
|Nov 2012
|dem in texas
|Nov 2012
|Nov 2012
|Nov 2012
|Nov 2012
|Nov 2012
Response to JRLeft (Original post)
Thu Nov 8, 2012, 12:38 PM
codjh9 (2,781 posts)
1. You'll probably get a more detailed answer, but from a TX native who just back out of the
state again 2+ years ago...
1) in the Prez race, Romney won by about 15% (yes, Ugh)
2) I don't know about dems in the state, but (1) tells you some of that. The Hispanic population continues to grow, and predictions are that it could be Blue in the next few/several years
3) BUT, supposedly - although in this election, nationwide, I think we had a good Hispanic turnout (not sure about TX per se) - Hispanic turnout in TX isn't what it could be
Response to codjh9 (Reply #1)
Thu Nov 8, 2012, 12:49 PM
JRLeft (7,010 posts)
2. Neither is African American turn out either.
Response to codjh9 (Reply #1)
Thu Nov 8, 2012, 03:47 PM
Blue4Texas (437 posts)
8. 15% begs for a strategy blue :)
Response to JRLeft (Original post)
Thu Nov 8, 2012, 01:01 PM
dem in texas (1,721 posts)
3. Re: Dems in Texas
I live in Dallas, one of the tiny blue spots on the red Texas map. Dallas, Austin, El Paso and San Antonio voted Dem but in all of these cases the surrounding suburbs are Repubs. Houston is Dem, but not as strong. The Rio Grande Valley has the strongest Dem areas. There was a great map in the Dallas Morning News today that shows how each county in the US voted (Dem or Repub). Traditionally, the Hispanics do not turn out in big numbers to vote in Texas, but I think that is changing. It is only a matter of time before the Hispanics make their voice heard in Texas and the Lone Star State turns blue. I am an old white lady Dem, a true "Yellow dog", straight ticket voter, and I hope to live long enough to see Texas turn into a Dem power house. Be patient folks, that day is coming.
Response to dem in texas (Reply #3)
Thu Nov 8, 2012, 01:13 PM
broiles (943 posts)
4. I heard that Houston went for Obama.
Response to broiles (Reply #4)
Thu Nov 8, 2012, 01:16 PM
JRLeft (7,010 posts)
5. I have to believe that there are enough voters in Texas to eek out a win if we could only get them
all out to vote.
Response to dem in texas (Reply #3)
Thu Nov 8, 2012, 01:22 PM
Robbins (5,051 posts)
6. Texas
It's the rural areas and city of forth worth that give republicans their advantages.
Dallas,Austin,el Paso,and San Antonio are voting democrats.
If Hispanics and Blacks get motivated to vote(some might think since Texas has been republican for so long their vote won't matter)
in larger numbers and more whites quit voting solid Republicans(39 percent of whites nationwide voted for Obama.There are white
democrats In every state Including Texas the problem Is we are minority among white voters)
It may take 2020 or 2024 but Texas will be a purple state eventully.If Republicans can't Immedelly count On texas they are Serious
trouble.LBJ thought civil rights would cost the democrats the south for a hundred years.By 2020 Texas and Georgia and possibly SC
could join Florida,Virginia and NC as battlegrounds.
Response to Robbins (Reply #6)
Thu Nov 8, 2012, 02:17 PM
JRLeft (7,010 posts)
7. Did you read the article I posted last night about the African American population explosion in the
the US? Black Georgia birth rate is extraordinarily high in Georgia and South Carolina.


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The grades of three Chemicals stocks are on the rise this week on Portfolio Grader. Each of these stocks is rated an "A" ("strong buy") or "B" overall ("buy").
Quaker Chemical (NYSE:KWR) is bettering its rating of C ("hold") from last week to a B ("buy") this week. Quaker Chemical produces, develops, and markets custom-formulated chemical specialty products. For more information, get Portfolio Grader's complete analysis of KWR stock.
This is a strong week for TPC Group (NASDAQ:TPCG). The company's rating climbs to B from the previous week's C. TPC Group provides specialized lines of chemical products to chemical and petroleum-based companies worldwide. The stock price has risen 9.2% over the past month, better than the 3.2% decrease the Nasdaq has seen over the same period of time. For more information, get Portfolio Grader's complete analysis of TPCG stock.
Huntsman Corp. (NYSE:HUN) is seeing ratings go up from a C last week to a B this week. Huntsman manufactures differentiated and commodity chemical products. For more information, get Portfolio Grader's complete analysis of HUN stock.
Louis Navellier's proprietary Portfolio Grader stock ranking system assesses roughly 5,000 companies every week based on a number of fundamental and quantitative measures. Stocks are given a letter grade based on their results — with A being "strong buy," and F being "strong sell." Explore the tool here.