text:
Accelerating Jumbo Mortgage Delinquencies Will Bash High-End Property Values: Part 1 of 6 – Anecdotes on Inventory
(Chicago) Look at unit sales over $1 million in Sarasota County Florida and all appears well. Twelve-month sales equaled 128 units at March 1 versus 151 units the previous year. The 15 percent fall in sales is real, but it isn't scary. If you want to sell your home there, you may not like the rest of the math as much.
Talk to Hannerle Moore, an agent at Michael Saunders & Co. She suggests a sobering strategy. Reduce prices at least 40 percent from 2005 highs.
"I tell them, 'You could be the lady who has had her home on the market for 936 days, or you could sell,'" Moore told the Sarasota Herald Tribune (Are High-End Properties Going Down? 4/26/10).
In putting together this story, I was unable to get all the data I was after on high-end inventory. I am firing shotgun to lead to something worth knowing. My review runs near and far in six posts starting today and includes most importantly data on the mortgage performance of jumbo mortgages. My hypothesis is that mortgage performance serves as a leading indicator of both future inventory and price trends. The worse the payment performance, the more prices will fall. Signs of serious distress on many other measures have been in open evidence for expensive properties and we will see it most clearly in jumbo mortgage performance.
Consider the statement of National Association of Realtors' chief economist Lawrence Yun. Almost exactly a year ago he said the supply of existing homes for sale over $750,000 had reached a forty-month supply (High-End Foreclosures Are Next, 5/27/09, CNBC). Translate that into something you understand: Inventory was SIX TIMES higher than it should be.
Inventory is the king of the castle. We need to analyze it in guessing the direction of future prices. The high-end market is always a small presence, but also of peculiar significance — to the owners of those properties.
Some stories of the log-jam in high-end property are dramatic. In Charlotte in 2009, less than 3% of all homes listed above $500,000 closed each month. In 2007, homes sales in that category closed at a 33% rate. My pigeon math says the old times were ten times better than the new times. How else to describe this but as a frightening fall off (High-End home lending market has Wells Fargo on line. 4/9/10. Charlotte Business Journal)?
The Wall Street Journal reported in a front page story that supply in June of last year of unsold homes priced above $750,000 equaled 17 months of sales from an already high 14.5-months the previous year (I don't know yet why Dr. Yun's report had the supply at 40 months from a story in the same time period.).
An agent from the wealthiest suburb of Chicago had an unmixed report on supply last August.
"We're extremely oversupplied," said Sherry Molitor, a real-estate agent in Kenilworth Illinois – the boyhood home of your HousingStory.net blogger (High-End Homes Frozen Out of Budding Housing Rebound. 8/3/09. WSJ).
She reports today (June 2010) that the Kenilworth market supply equals 18 months, down from 22 months a year earlier. The average selling-period is 349 days and 49 homes are listed for sale of a total of 800 households. Ms. Molitor believes values have fallen about 25 percent in Kenilworth and in the other wealthy suburbs of the North Shore of Chicago (Wilmette, Winnetka, Glencoe, Highland Park, Lake Forest).
High-end Dallas also looks long in tooth. At the end of this March, listings over $500,000 provided 20 months of supply and 4500 units. Overall that market had six months of supply. This obvious indicator of high-end distress may be true in all markets across the United States. Each one is different and needs to be studied on its own, but national trends are real and must also be considered.
The element of subterfuge is highest in expensive neighborhoods. You can expect ambitious concealment when a thing which requires massive sacrifice – blowing 5k or 10k out the door every month just to keep running in place — has a price falling in the wrong direction.
One Russell Shaw of John Hall & Associates (self-reporting as in the top 1% of all property agents nationally) says that real estate owned by banks following a foreclosure is big business in high-end homes and the only game in town for asset managers whose work is reselling homes lost after payment failure and bank seizure.
"Those high end agents are getting inventory, lots of it," Mr. Shaw said (The Shadow Inventory Equals Shadow Gibberish. 5/8/10, AgentGenius.com).
The fall in price can be wicked. If you think your digs are north of $13 million in value, take note that a Walla Walla Washington residence boasting 15,000-square-feet listed for $13 million and closed last spring for $3.5 million. Truly a Hannibal haircut (You remember the scene where one's scalp is now outer brain – as the scalp has been neatly removed by Hannibal. So much better for your brain to take in the sun and produce vitamin D.).
Beware the ghost of Patty Hearst (and headless Thompson gunners who prey upon teenage girls). Veronica Hearst, the widow of Randolph and stepmother of SLA Patty, she kicked back to the bank her 52-room oceanfront Palm Beach Florida cottage. The mortgage on it: $22 million. For the curious, the monthly payment there is $131,901 based on a rate of 6% and a 30-year amortization.
Here in Chicago, the land of Lincoln and Blagojevich, big hair will take you to the governor's mansion. On the other hand, real work over long periods of time is required to off-load either the $3-million-type inventory or the $5 million-category inventory.
In Chicago's land of horses, Lake Forest, more than 50 homes were listed for sale for at least $3 million last fall. Yet in the prior 12 months only 11 residences in that price range had sold. Typical marketing period: 500 days.
Rise up to homes listed for at least $5 million and there were more than 20 Lake Forest properties for sale. Yet the 12 month-clearance on these super-fives brought only one little close. The Chicago Tribune called this a 20-year inventory (Ultra High-End Homes Slow To Sell in Crowded Market. 10/18/09). I haven't heard of that number before, but essentially it means selling your home requires the same planning, patience, and follow up as saving for college when your newborn first arrives and carrying through all the way to graduation.
So what do we know so far about the direction of inventory of expensive properties? A pro says to knock down prices 40% to list in Sarasota County. The National Association of Realtors reported a high-end supply of 40 months — or more than SIX TIMES higher than it should be. Charlotte is selling out ten times slower than in better times. Kenilworth Illinois is "extremely oversupplied". And in Lake Forest power houses are selling, but it takes 20 years to get rid of the thing. Does anybody see a trend? I have one question for you Mrs. Lincoln: Did you love your husband?
PRINT – Part 1 Jumbo Mortgage Delinquencies Bash High-End Property Values
Check back later this week for Part 2 of our six part series "Accelerating Jumbo Mortgage Delinquencies Will Bash High-End Property Values".
Mark Mallinson permalinkJune 17, 2010 1:06 pm
Good job Mike,
Here is some data for when you cover Malibu & Beverly Hills
The following news articles reflect current ultra-luxury housing trends:
Los Angeles Times on 2/20/2010
"Luxury meets reality as asking prices tumble: High-end sellers find they're not immune to slump."
"Southern California real estate landscape has been littered with its share of high-profile price drops.
Nicolas Cage's 11,817 sq.ft. English Tudor in Bel-Air has been reduced 50% to 17.5 million from $35 million when it first hit the market in 2006. Now, even the wealthy are facing a new reality as some luxury homes' prices have dropped – and dropped again- over the last few years and agents are begging sellers to be realistic in setting an asking price. The act of repeatedly lowering the asking price until a buyer is found is known as "chasing the market." Seller' thinking has been slow to adjust to the sea change in pricing since the bubble burst." (LA Times 2/20/10)
(Footnote: Nicolas Cage's property was foreclosed in March of 2010.)
Business Insider. com reported on 6/3/2010: Re: An oceanfront Malibu Beach property.
"Remember the Wells Fargo Party House? It's for sale at $3 million off."
"Wells Fargo rushed to sell the house, hiring reality star/real estate agent Chad Rogers (of Bravo's show Million Dollar Listing).
The asking price was $21.5 million. Fast forward a year later, the four bedroom, four-bathroom house is still on the market and currently listed for $18 million. Any takers?"
Jon permalinkJune 16, 2010 1:00 am
Hey Mike,
The following link (drhousingbubble.com) shows 10% of foreclosures in the LA area are 2-4 unit properties:
Jon permalinkJune 14, 2010 9:35 am
Another fantastic post !! I look forward to the next part (and the next one, etc.)…
I am hoping (anyone voting with me?) that at least one part of this series addresses investment properties, particularly 2-4 family homes.
In many areas (my personal experience is in Boston and surrounding towns, but I am sure it is true in other cities as well), in the better neighborhoods these properties have become "boutique" purchases, in the sense that there is no business case (positive cash flow? not even close to break-even, despite being fully rented on a continuous basis) for a non-cash buyer.
I am suggesting that over the last 5 years, buyers of these properties were subject to the same bubble mentality as everyone else.
Robert permalinkJune 14, 2010 6:32 am
This is a very interesting article. In my little market in middle New Hampshire we have a 5 year supply of hi end > $700,000 homes on the market. This is based on 2009 sales figures. These homes are owned by relatively wealthy people who may be able to wait it out as we have had little price deflation. We will either have a flat market for 30 years or prices will have to come down. This market is being severely affected by the credit crunch as buyers with good incomes who would have come in looking at $900,000 or $1,000,000 houses two years ago are now looking at $450,000 to $600,000 houses. They used to be able to buy the more expensive homes with $50,000 – $60,000 cash down. They are only looking at the lower end because they still only have $50,000 to $60,000 to put down.
- S&P/Case-Shiller Home Price Index keeps going up. National AVG+4.6%. California - SF+18.3%, SD+12.4%, LA+9.7% - Real Estate -Brokers, appraisals, development, lease, investing, relocation, apartments, houses, condos, values, mortgages, loans... - City
Comments are closed.


text:
Young drivers clocked doing over 180kmh
Page Tools
Two young motorists have been clocked driving more than 180kmh on the Hume Highway in northern Victoria.
The drivers, who had their licences automatically suspended for a year, were intercepted together just after 6am yesterday.
Police said an 18-year-old Wangaratta man on P-plates travelled 184kmh in a Subaru, while a 21-year-old man, also from Wangaratta, drove a VT Commodore at 186 kmh.
They were driving in a 100-kmh zone near Wangaratta.
Get the SMH delivered for as little as $3 a week - SAVE 21%

subset: commoncrawl


text:
The market continued where it left off last Friday and continued higher today! Right out of the gate, better-than-expected economic data, including manufacturing, consumer spending and construction spending, encouraged investors to work more sidelined money back into the stock market. Techs were especially strong, led by the semicondutors (mentioned last night in my Sector Watch). Internet stocks also showed strength, with BIDU catapulting +8.41% and AMZN "squeezing" up +6.49%! Miners also contributed in a big way: X +8.04%, FCX +6.78%, and ATI +9.04%! Oil services rose higher again, with DO adding +5.83%, allowing us to cash out our remaining position at +158% gain:
07:03 | HappyTrading DO ($87.84) Sold to Close DOFF Jun 80 calls, at $8.50 +158%
We also took profits on BNI and ISRG:
08:43 | HappyTrading ISRG ($153.00) Sold to Close AXQFJ Jun 150 calls, at $8.20 +39%
08:27 | HappyTrading BNI ($76.00) Sold to Close BNIFN Jun 70 calls, at $6.50 +71%
These trades were opened on Thursday and Friday last week:
May 29, 2009
12:29 | HappyTrading BNI ($72.25) Bought to Open BNIFN Jun 70 calls, at $3.80
07:10 | HappyTrading ISRG ($148.30) Bought to Open AXQFJ Jun 150 calls, at $5.90
May 28, 2009
08:07 | HappyTrading DO ($80.30) Bought to Open DOFF Jun 80 calls, at $3.30
The Dow closed up +221.11 points; SPX added +23.73 points; and, Nasdaq jumped +54.35 points:
As mentioned earlier, SOXX (semiconductors) led the techs with a +5.31% gain. USO (oil) added +2.83% while OIH (oil services) gained +4.88%. XME (metals and mining) rose +6.81%. XLE (energy) glided up +3.68%. GLD (gold) and SLV (silver) were both in the red. PBW (clean energy) rallied +6.21%. FXI (Chinese ADRs) advanced +5.78%.
SPX added +23.73 points to close at 942.87. It caught up with its daily upper BB. The MACD went higher.
Nasdaq jumped +54.35 points to close at 1828.68. The daily MAs and MACD curved up!
In my Market Forecast last night, I mentioned that, this week, "SPX should look too push above 950, although it may see some resistance around that level. Nasdaq can easily punch through 1800..." SPX got as high as 947.77 today and Nasdaq finished above 1800. Both indices closed above their respective daily upper BBs (Bollinger Bands). Their MACDs are showing new bullish crossovers, which we have discussed recently. Also, new bullish fomations are established in the daily MAs. The market rallied hard and backed off a bit near the close. VIX, surprisingly, closed a bit higher and above 30. But, nothing to be too alarmed about as last Friday's drop in VIX was frantic. SRS (real estate ultrashort ETF) closed below $20 on a new leg down and SKF (financial ultrashort ETF) barely kept above $40. I think the market's new leg up is just starting. We may see some morning volatility tomorrow, but, I think this new leg up should continue higher. Nevertheless, we played things safe and cashed out on the big gains. We're holding mostly cash and will wait to see how the market looks tomorrow.
Good night and HappyTrading! ™


text: The Patrick Stewart wiki last edited by Yummylee on 05/25/14 06:12AM View full history Patrick Stewart is a British born classically trained actor who has starred in a multitude of TV, film, and stage productions. Stewart was born on Yorkshire, England on July 13, 1940 and entered drama school at the age of 17 starring in a wide array of stage productions for a variety of companies. He is most well known for portraying Captain Jean-Luc Picard on Star Trek: The Next Generation. The show ran from 1987 to 1994 and has also been adapted into four major motion pictures. He continued his roles in sci-fi by playing Charles Xavier in the X-Men films. He has also appeared in many other works, including Shakespeare plays.He has voice acted in many games, from reprising his roles as Jean-Luc Picard and Charles Xavier, to appearing as Emperor Uriel Septim VII in The Elder Scrolls IV: Oblivion.


text:
Bamboo Flooring Carbonized
When we were looking for flooring, we wanted to be environmentally conscious and stay away from hardwoods and exotic flooring. We stumbled across Yanchi bamboo flooring at BuildDirect and fell in love with the Strand Woven flooring. It looks like hardwood, is as hard as oak, and is environmentally friendly. Being skeptical of purchasing large tickets online, we called the company (BuildDirect) and they were great! A super product at a super price with super customer service. A win-win-win!!!!


text:
Better for Himpunan Hijau to remain non-partisan
YOURSAY 'It is Wong's personal choice to become a politician but he must realise once he is a member of a party, his principles will be compromised.'
Quit Himpunan Hijau, panel member urges Wong Tack
Anon.ymous: Himpunan Hijau steering committee member Clement Chin's request to Himpunan Hijau chairperson Wong Tack is perfectly logical.
Himpunan Hijau should continue to work outside the establishment and not let its hands be tied to any political master.
Ann: Yes, much as I want political change, I have my doubts as to how committed Pakatan Rakyat parties really are to the environmental cause.
The ambiguous recent statements by their leaders over whether they would really shut down Lynas seriously worries me. They seem to be back-tracking from their earlier unequivocal call for the plant to be shut down, much as I feared.
Himpunan Hijau should not be linked to the DAP, which is pursuing unsustainable policies in Penang (tunnel, highways) and seems to be co-opting Wong and the green cause for their own political ends.
KL112: Wong has to understand that once he is elected as an MP, he needs to speak on many issues that effect the rakyat, not just environmental issues. As an MP, he can give full support for Himpunan Hijau, even if he is not the chairperson.
Ferdtan: Chin, I think you should quit Himpunan Hijau instead, not Wong, as he has the endorsement of the whole committee. If you cannot agree with the whole committee, then you should resign.
You just cannot see the big picture of Wong contesting in the coming election. It is a means to an end - to put for the very first time a 'Green MP' in Parliament.
BN has totally rejected Himpunan Hijau and since Pakatan supported the movement by offering a seat for Wong to contest in, what is wrong with that?
If Chin doesn't understand the difference between a candidate contesting under a particular party's political ticket and being a full-time member, he is not fit to be in the Himpunan Hijau committee.
Sungai Siput MP Dr D Jeyakumar of Parti Sosialis Malaysia (PSM), contested and won under the banner of PKR in 2008. Did you see him be under any obligation to follow the dictates of PKR?
Nes: It is Wong's personal choice to become a politician but he must realise once he is a member of a party, his principles will be compromised. He should have quit Himpunan Hijau once he had made up his mind.
By not doing so, he lost credibility, especially so when he immediately agreed to allow Lynas to operate if done through the 'front door' (whatever that means), when not so long ago he wanted to burn it down.
However, you have our blessing as a politician. Very few politicians serve the people, but instead serve themselves. I hope you are one of the few.
Sabahan: Wong will need to stand down only if he is elected, as he will not have time to lead Himpunan Hijau. If he is defeated, he can continue leading the movement. There is no law that NGO leaders have to resign before standing for elections.
Let us not put conditions on potential candidates. Even if he resigns, he will have the support of Himpunan Hijau.
Lie detector: Chin, Wong is being controlled by party ideology? He is contesting on a DAP-Pakatan ticket. The Pakatan manifesto is need-based, not race-based, anti-cronyism, deficit reducting, anti-corruption, greater transparency, and anti-Lynas, etc. Indeed, please let me be controlled by Pakatan.
On the contrary, Chin, since you don't support Wong, you should resign from Himpunan Hijau. Check out the crowd last night who gave their massive endorsement to Wong.
LiarsSlaughter: Wong's acceptance of the offer of the candidacy in the GE13 is another way for Himpunan Hijau to achieve its goals. Don't compare Hindu Rights Action Force (Hindraf) with Himpunan Hijau.
Wong is being offered a seat without requesting for it. Whereas Hindraf's leadership is demanding for seats and threatening to sabotage Pakatan if it doesn't get them.
Ian2003: Very commendable and totally agree. Hopefully, Hindraf would learn something here.
Senior: Be apolitical. If you want to be an MP, give up your environment activities. I have no love for BN or Umno, but on principle, politicians and environmental issues don't mix, unless you form a Green Party, but not in DAP.
Wira: Wong is beholden to no political party. Just that he is furthering the cause of Himpunan Hijau and is standing in Bentong as a representative of the green movement under a DAP ticket and the umbrella of a two-party system.
Do you see Perkasa president Ibrahim Ali beholden to PAS even though he stood under a PAS ticket in 2008? Pakatan feels Wong is a good bet because this honourable gentleman stands a good chance to deprive MCA-BN of their safe seat in Bentong.
Pakatan will suspend the Lynas plant should they win Putrajaya and the suspension will remain so unless Pakatan-instituted inquiry can prove it to be safe. Isn't this synonymous with what the green movement wants with respect to Lynas?
With BN, the green movement stands zero chance of shutting down Lynas and the movement had already exhausted all legal avenues.
Low Kok Kok: The moment he joined politics, Wong loses his credibility. For someone who threatened to burn down Lynas and now agree to Lynas if they use the "front door" shows that he is a person who is willing to compromise his principles.
Secondly, how could DAP accept him as a candidate when he threatened to burn down Lynas - it was grossly immature of him. At his age, he cannot behave like a loose cannon and simply fire away in Parliament.
Cowboy: Wong is the most suitable candidate to contest for the Bentong parliamentary seat. He represents Himpunan Hijau's cause and aspiration. DAP is just providing him the platform.
Let the voters decide. If he is successful, that he will send a powerful signal to Parliament that people are concerned with environmental issues.
The above is a selection of comments posted by Malaysiakini subscribers. Only paying subscribers can post comments. Over the past one year, Malaysiakinians have posted over 100,000 comments. Join the Malaysiakini community and help set the news agenda. Subscribe now .
Related stories
Wong Tack's political career can affect green movement
Bentong now within striking distance for DAP
PKR will let go of Bentong for two state seats


text:
E. Wayne Gent traces his call to the teaching profession back to his parents, who were long-time Sunday school teachers and important influences in the lives of their students. Through them, he developed a passion for education and a particularly soft heart for students who, like his parents,
WEST PALM BEACH— Palm Beach Atlantic University will honor Palm Beach residents Susie Elson and Nancy Murray as Women of Distinction 2014 at a luncheon Wednesday, Feb. 19, 2014, at The Breakers, Palm Beach. A pre-luncheon reception begins at 11:15 a.m.; the luncheon follows at noon. Tickets,


text:
ATLANTIC CITY, N. J. (Aug. 26)
The 34th Democratic Party national convention approved here last night a platform for the 1964 campaign pledging vigorous support for Israel, deploring Soviet persecution of Russian Jewry, and calling for revision of the United States Immigration and Naturalization Act to eliminate the "discriminatory" national origins quota system.
The platform also denounced extremism, "whether of the right or the left," assailing specifically "the extremist tactics of such organizations as the Communist Party, the Ku Klux Klan and the John Birch Society."
The immigration plank asserted that "the immigration laws must be revised to permit families to be reunited, to welcome the persecuted and oppressed, and to eliminate the discriminatory provisions which base admission upon national origins."
Another plank on domestic issues called for the elimination of all discrimination in the field of employment based on race, color or religion.
The Near East plank pledged the party to "work for the attainment of peace in the Near East as an urgent goal, using our best efforts to prevent a military unbalance, to encourage arms reductions and the use of national resources for internal development, and to encourage the resettlement of Arab refugees where there is room and opportunity. The problems of political adjustment between Israel and the Arab countries can and must be peacefully resolved and the territorial integrity of every nation respected."
The denunciation of Soviet harassment of Jews in Russia was contained in a plank which pledged to encourage by all peaceful means the growing independence of captive peoples living under Communism, and to hasten the day when captive nations would achieve full freedom and self-determination. The plank said specifically that "we deplore Communist oppression of Jews and other minorities."
Another portion of the platform outlined the determination of the United States to Israel through cooperative research in the application of nuclear energy for desalting sea water.
A supplement to the platform, "An Accounting of Stewardship," which reviewed the party's accomplishments, cited the 1960 Democratic plank pledging pursuit of Arab-Israel peace by preventing an arms imbalance. The supplement declared that this policy had brought the Arab countries and Israel "closer to peace and stability than at any time since World War II."
The platform declared: "Economic and technical assistance to Israel and Arab nations continues at a high level, although with more and more emphasis on loans as against grants. The United States is determined to help bring the revolution in the technology of desalination to the aid of the desert regions."
The platform also reviewed United States participation in the United Nations to strengthen the UN's capacity as a peace keeper, mentioning the continued UN "guard on armistice lines" in the Near East. The "guard" referred to the United Nations Emergency Force, which protects Israel from Egyptian attacks by being posted on the Egyptian side of Israel's Gaza Strip border, and along the heights of Sharm el-Sheikh, in the Sinai Desert. The latter UN outpost guarantees Israel's freedom of shipping in the Gulf of Akaba.


text:
The Leach's Petrel, murre, and some other sea birds, have but one egg.
Coolidge's party went to the murre Rocks, where the Guillemots breed, and brought about fifteen hundred eggs.
Murrain (or morena in Latin chronicles) is probably the modern survival of mure or murre.
When the mouthe turneth in speakinge it is a sygne that it is infected with some catarre or murre as is manyfest ynough.
The gull is soon through, but the murre continues to lay for about two months.
The gull begins to lay about the middle of May, and usually ten days before the murre.
The murre does not fly high, nor is it a very active bird, or apparently of long flight.
In the fifteenth century the English name was mure or murre, which appears to be the same root as in murrain.


text: David Landers plays the voice of Henry the penguin on the kid show "Oswald" (Oswald the octopus is voiced by Fred Savage) playing on Nickelodeon. I sometimes watch it with my 3 year-old. One episode had Henry's cousin come in from out of town. His cousin was voiced by Michael McKean (who played Lenny.) I can't tell you how funny it was to hear Lenny and Squiggy pestering eachother in a children's show - while appearing as penguins!Link


text: >qsub -X -I -q long Thanks. It is now working Regards, Mahmood ________________________________ From: Shenglong Wang <shenglong.wang at nyu.edu> To: Torque Users Mailing List <torqueusers at supercluster.org>; Mahmood Naderan <nt_mahmood at yahoo.com> Sent: Saturday, August 18, 2012 9:29 PM Subject: Re: [torqueusers] gui program does not work in interactive mode Try qsub -X -I -q long On Aug 18, 2012 12:14 PM, "Mahmood Naderan" <nt_mahmood at yahoo.com> wrote: Dear all, >It seems that running a graphical program (which uses X) is not possible in the interactive mode. >1) on the server, i run "ssh -X ws01" and then run the program. Everything is fine and I see the main gui of the program >2) on the server I run "qsub -I -q long", then I enter ws01. This time when I run the program, it says can not open the display. > >Any thought on this? > > >Regards, >Mahmood > >_______________________________________________ >torqueusers mailing list >torqueusers at supercluster.org >http://www.supercluster.org/mailman/listinfo/torqueusers > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.supercluster.org/pipermail/torqueusers/attachments/20120818/bdf8cd57/attachment.html


text:
Not weekly, officially.
Quickly Generate Random Dates in Ruby
It's easy to use and obviously gives you more varied results than doing Time.now like most of us do all the time we need a date. Just say Time.random instead.
This blog post is in response to a lot of well-deserved confusion in the community around CouchApps. We haven't been clear enough in the past (either in technical description or in the notion of the project). I hope to change all that (with your help). This is just the beginning.
Bootstrapped, Profitable, & Proud: GitHub
Q&A with Chris Wanstrath, CEO and Co-Founder of GitHub. This is part of our "Bootstrapped, Profitable, & Proud" series which profiles companies that have $1MM+ in revenues, didn't take VC, and are profitable.
Introducing rollout: Condionally roll out features with redis
When we're ready to alpha the feature, we'll roll it out to staff. For beta, we might roll it out to some specific friends or people who request access. Then, when it's time to go live, we'll roll it out to a percentage of people at a time to make sure that any remaining performance issues are caught without bringing down the entire application. If we do find a problem, we need to be able to disable the feature in real-time. We do all of this using a tool we put together called rollout.
Auto-scale your Resque workers on Heroku
This app I'm working on, I'm hopefully going to have to pay for at some point, as I hope enough people will want to use it that the free stuff from Heroku just won't cut it. However, the less I can pay the better, and background job workers aren't free on Heroku. They are, fortunately, billed by the second.
Resque is a queueing system that is backed by Redis. Common use cases include sending emails and processing data. This tutorial will cover setting up Resque with Rails and Redis To Go [and Heroku]
Successful fundraising with Kickstarter.com & (re)making Art Space Tokyo
Ian McFarland, Principal and VP of Technology for Pivotal Labs, reprises his popular RailsConf 2010 talk. Ian describes the technical and social aspects of how Pivotal practices agile software development. [interesting talk. Subscribed to the podcast, too.]
Enslaving Branches: How GitHub Does Enterprise
FI is aimed at larger companies that want to host their own version of GitHub on their own hardware. We ship them a full, self-contained stack, and once installed they have their own private github.com on their network.
The Acceleration of Addictiveness
Most people I know have problems with Internet addiction. We're all trying to figure out our own customs for getting free of it.
No Callbacks, No Threads & Ruby 1.9
In the talk he discusses the state of the Ruby VM and why we should standardize an asynchronous Ruby stack which takes advantage of Ruby 1.9, Fibers, and non-blocking database drivers to make Ruby (and Rails) more scalable.
500 Million Stories | Facebook
As of this morning, 500 million people all around the world are actively using Facebook to stay connected with their friends and the people around them.
Bad mornings are the ones where I sit at home compulsively unbolding things hoping that somewhere in there there will be the gem of connection and stimuli that gets me out the door.
…and I grieve for the spirit of Work, killed by her evil child, Workflow.
[Makes me want to take another look at Camping, since I only checked it out when I was just getting started with Ruby.]
Why is NoSQL generating so much buzz? What does it mean for you, the application developer? And what place does NoSQL have for apps running on the Heroku platform?
Fantastic file uploading for your web application. [Resize images, encode videos, extract thumbnails, store in s3.]
a simple online notepad
YouTube to Mp3 Converter and YouTube Video Downloader
I spoke at the O'Reilly Velocity conference this afternoon about using version control to manage web services.
This view plugin adds AM/PM (12 hour time) support to the core DateHelper methods.
This document showcases many of the latest developments in PostgreSQL 9.0, compared to the last major release – PostgreSQL 8.4. There are more than 200 improvements in this release.
Why does Quora use MySQL as the data store rather than NoSQLs such as Cassandra, MongoDB, CouchDB, etc.?
What were the considerations they took into account when choosing MySQL as the data store?
InfoQ: 7 Lessons Learned at Reddit
Steve Huffman, co-founder of Reddit, shares the main lessons he learned scaling Reddit from a small web application to a large social website.
There are many ways to use this elaborate hash table and many ways which are more trouble then they are worth. In our experience the key to use memcached effectively is to ask it for the exact thing you want, but i'm getting ahead of myself.


text:
I know that whenever I used to drink soda pop, I would pee quite a bit. I would have to pee quite frequently. And so that's why I quit drinking soda pop... that way I don't have to pee a lot. It's been rather nice... not having to have to run back and forth to the urinal. Now I get to hang out and enjoy life without running back and forth to the urinal. It has been rather nice.
Absolutely... I remember this one time I was in the middle of acting out a scene in "Meet Joe Black", and I felt like I needed to pee really bad... because I had consumed 2 liters of root beer. Sure enough, I ended up wetting myself on the set. It was embarrassing, and to this day, I have never lived it down. So there you have it.
Well I don't know if its this way for everyone but when I drink a 64oz root beer it seems like I have to pee more than I do when I drink nothing. Usually the urine is also clear which means that the root beer has completely hydrated me so it must be really good for me. I would suggest to all professional athletes and people everywhere to drink 64oz soft drinks directly before any physical activities such as walking or the olympics.
Haven't you heard...what!??! You haven't heard...of the bird?! Oh wella bird, bird, bird, bird is the word!!! You haven't heard about the bird...Well...Pop does make me pee a lot, quiet a bit my friend Haamid.


text:
To have used his razor(31 Posts)
Cheeio26 Tue 12-Feb-13 19:40:14
HollyBerryBush Tue 12-Feb-13 21:53:26
OHforDUCKScake Tue 12-Feb-13 21:53:28
ThereGoesTheYear Tue 12-Feb-13 21:55:36
Fakebook Tue 12-Feb-13 21:56:36
OHforDUCKScake Tue 12-Feb-13 21:57:21
WheresMrMonkey Tue 12-Feb-13 22:39:38
Join the discussion
Registering is free, easy, and means you can join in the discussion, get discounts, win prizes and lots more.Register now
Already registered with Mumsnet? Log in to leave your comment or alternatively, sign in with Facebook or Google.
Please login first.


text:
(Received 28 October 2011; accepted 15 March 2012)
Published Online: 2012
Cite this document
This paper describes the results of a study that used the flow number (FN) test to evaluate the rutting susceptibility of asphalt concrete (AC) mixtures containing recycled asphalt pavement (RAP). The study compared the rut depths determined using an asphalt pavement analyzer (APA) (APA rut depth) with binder stiffness test data obtained with a dynamic shear rheometer. Both APA rut depths and binder stiffness are used to evaluate the rutting susceptibility of AC mixtures in Virginia. However, recent studies suggest that the results of the FN test might correlate better with field rutting than most existing rut tests. In addition, because currently almost all AC mixtures produced in Virginia contain RAP, extraction and recovery (ER) tests are required to obtain binder for testing. Therefore, another objective of the study was to determine whether the correlation between FN and the stiffness of recovered asphalt binders would allow the FN test to be used as a surrogate for the recovered binder stiffness. For the mixtures evaluated, the APA rut depth ranged from 0.61 to 1.99 mm, the FN from about 700 to 7500 cycles, and the binder stiffness from 1.637 to 8.498 kPa. The results show that FN was positively correlated with binder stiffness (r = 0.8507, p < 0.0001) but not correlated with APA rut depth (r = −0.2666, p = 0.3010). The results also show that APA rut depth and binder stiffness were not correlated (r = 0.01829, p = 0.944). The study concluded that FN is sensitive to binder stiffness for the mixtures considered. This suggests that the FN test could be used as a new approach for evaluating the effect of RAP on asphalt mixtures and thereby replace or minimize the use of binder ER test methods.
Author Information:
Apeagyei, Alex K.
Research Scientist, Virginia Center for Transportation Innovation and Research (VCTIR), Charlottesville, VA
Diefenderfer, Stacey D.
Research Scientist, VCTIR, Charlottesville, VA
Stock #: JTE104471
DOI: 10.1520/JTE104471Author


text:
Take Back the Night 2012
Release date: March 20, 2012
Take Back the Night comes to Goucher College to shatter the silence surrounding sexual violence and offer survivors a place to be safe, speak out, and be heard.
Tuesday, March 20
Self-Defense Workshop
9 p.m., Heubeck Multipurpose Room
Male and female students are invited to attend a self-defense workshop to learn how to fend off an attacker. The class will be taught by Annie Cutchen '14, a self-defense instructor and organizer of Goucher's mixed martial arts club. The class is appropriate for all fitness levels, but participants should wear comfortable clothing.
Clothesline Project
10 p.m., Heubeck Multipurpose Room
Goucher community members can contribute to the Clothesline Project, which allows those who have been targeted by sexual violence and those who oppose sexual violence to decorate and/or write their stories on a T-shirt that will be hung on a public clothesline. The exhibit will be displayed in the Residential Quad and along the outside of Van Meter Hall during the week of March 19. In the case of rain, the shirts will be exhibited in Pearlstone Atrium.
Thursday, March 22
8 p.m., Pearlstone Atrium
Take Back the Night will officially open with a performance by the Reverend's Rebels, Goucher's female a cappella group. Guest speakers Lisa Barken and Phil Jacobs, survivors of childhood sexual abuse, will speak on behalf of the Shofar Coalition, an organization that aims to prevent the physical, sexual, and emotional abuse or neglect of children and to aid adults, adolescents, and children who are suffering from the impact of traumatic experiences.
Community Speak-Out
8:30 p.m., Pearlstone Atrium
Take Back the Night will continue with an open forum for students and other members of the Goucher community who have been affected by sexual violence to share their stories. Those who wish to speak out are encouraged to do so; volunteers will also be on hand to read aloud personal accounts that are shared in writing.
March to End Violence
9:30 p.m., Through the Residential Quad
Participants will assemble in the Pearlstone Atrium for a symbolic march through the residential area of campus, highlighting everyone's right to live, study, work, and socialize without fear of sexual assault or harassment.
Healing Service
10 p.m., Haebler Chapel
The march will conclude at Haebler Memorial Chapel, where there will be a healing service with readings, reflection, song, and silence. Survivors, their supporters, and all those affected by sexual violence are welcome.
* Peer Listeners also will be available nightly until 2 a.m. at 443-632-7799.
Take Back the Night is sponsored by Student Health and Counseling, the Dean of Students Office, the Office of Religious and Spiritual Life, the Office of Student Engagement, the Office of Public Safety, the Student Government Association, PRISM, Hillel, Feminist Majority Leadership Alliance, Green Dot, Goucher College Peer Listeners, Counseling, Helpline & Aid Network for Abused Women (CHANA), and the Shofar Coalition.
For more information, contact Zachary Kohn at firstname.lastname@example.com.


text:
The Farmers Irrigation District developed a screen technology that keeps fish from entering irrigation and hydroelectric canals. The Farmers Screen is a horizontal, flat-plate diversion screen that harmlessly moves fish over the screen and back to the river while safely diverting water for irrigation and hydroelectric use. The Farmers Screen is gaining widespread acceptance by natural resource agencies, farmers, and power producers alike. The Farmers Screen technology is licensed to a non-profit group called FCA with the condition that money from screen sales be used for the benefit of fish, farms and families.
Click here to go to the FCA website and learn more about the Farmers Screen, or click here to watch the Farmers Screen on our live fish screen webcam.
Image used by permission from FCA Solutions


text:
Alphabet Letter W Woodpecker
Preschool Lesson Plan Printable Activities, Worksheets and Craft
Alphabet >
Crafts > Letter W
Animals >
Birds > Endangered Animals > Forest
Holidays and Events >
Jan 5th > National Bird Day
May 4th > Bird Day
Here are printable materials and some
suggestions to present letter W.
Animals > Bird > Endangered > Forest > Woodpeckers
A woodpecker is a bird with a hard, pointed bill for making holes in trees to find insects to eat and to communicate. Their strong claws and stiff tails help them climb trees. Woodpecker are of many different sizes and are found around the world.
Online resources > Woodpecker @ Wikipedia.org
Online Jigsaw Puzzle > Woodpecker > Skills: Problem solving, bilingual English and Spanish
Here is an online puzzle the children will enjoy with a fun woodpecker image. The default puzzle is 6 pieces. Click on the change cut button to change shape and number of pieces.
Alphabet Activities > Letter W is for Woodpecker
Present and display this cute color poster and your option of printable activities in the materials column.
Children Age 3 and under >
* Print a letter W coloring page D'Nealian or Standard Block and a woodpecker coloring image 1 or 2 behind it or on a separate page if using paints to decorate later. Discuss other letter W words found on the worksheet.
* Finger Tracing: Trace letter W's in upper and lower case with your finger as you also sound out the letter. Invite the children to do the same on their coloring page.
* Children can trace and color the letter W's. After completing coloring the letter, encourage children to color the woodpecker image and write the word woodpecker on the finished coloring page.
Children Ages 3+ to 6: Present the Letter W Worksheet and Mini Book program. Read suggested instructions for using the worksheet and mini-book. These materials can be used as part of Letter W program of activities to reinforce letter practice and to identify related W words.
Display letter W posters and coloring pages or make a letter W classroom book. For related images visit: letter W printable activities.
Letter W Word Search: The four word search game features a woodpecker and letter W words with pictures and handwriting practice.
Advanced independent handwriting practice:
1. Print your choice of printable lined-paper and encourage children to draw a woodpecker behind the page or print a woodpecker coloring image 1 or 2 image.
2. Drawing and writing paper: Encourage children to draw and color a woodpecker and write letter W w. Share some images of real woodpeckers to inspire the children.
Crafts > DLTK's Printable Woodpecker Craft > This printable craft showcases a pileated woodpecker, and it is assembled using a toilet paper tube.
|To view updates to these activities visit: http://www.first-school.ws/activities/alpha/w/woodpecker.htm


text: FRANKFURT, Germany ( TheStreet) -- Daimler ( DDAIF), the German carmaker, said second-quarter earnings rose 30% to €1.7 billion ($2.46 billion) from €1.31 billion a year earlier as the Mercedes brand posted record profit. Daimler said revenue rose 5% to €26.3 billion. Mercedes recorded its strongest ever quarterly earnings before interest and taxes of €1.57 billion, up 14% from a year earlier. Daimler, in a statement Wednesday, said group EBIT in 2001 "will very significantly exceed the level of 2010." "With our excellent first half of the year, we are fully on schedule to turn 2011 into one of the most successful years in our long corporate history," said CEO Dieter Zetsche. Profit also rose at Daimler's truck division, Mercedes van division, and financial services division. -- Written by Joseph Woelfel >To contact the writer of this article, click here: Joseph Woelfel >To submit a news tip, send an email to: firstname.lastname@example.com.


text:
This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Members of the marine dinoflagellate genus
Phytoplankton in aquatic systems face various survival challenges, some of which result from grazer attack, pathogens and competition with other microalgae. The performance of particular species in these inter-specific interactions is assumed to play an important role in determining which species will survive and even to grow rapidly enough to form aggregations (
Marine dinoflagellates in the genus
The search for the chemical nature and structure of allelochemical substances that mediate effects among marine protists, based on classical bioassay-driven fractionation have often resulted in loss of activity or led to irreproducible or inconclusive results. The aim of this current study was to investigate the basic properties and the physico-chemical behavior of these poorly examined compounds, tracking activity with an ecologically realistic target bioassay organism. The lytic activity of cell free supernatant of
After storage of diluted (1:10) cell-free culture supernatant in 20 mL glass vials under normal culture conditions (15 °C), lytic activity rapidly declined, with an almost 50% reduction after 4 days and a complete disappearance after 7 days (
Lytic activity was however easily reestablished by shaking the containers vigorously before removal of stored supernatant. Even after 14 days the apparent loss of lytic activity could be reestablished by shaking. With a vigorous shaking before sub-sampling, a detailed quantitative experiment on stability under normal culture conditions was then performed. For the initial sample, the dilution curve (
As shown above, lytic compounds are stable at room temperature and also did not lose lytic activity upon freezing. After storage at −20 °C for three months, lytic activity was still high (
Supernatant could also be lyophilized without losing activity; the EC50 value of lyophilized and re-dissolved sample was 0.4% (0.4–0.5%), which was only a slight decrease compared to untreated original supernatant [EC50 = 0.3% (0.3–0.3%)]. With respect to heat stability, when exposed to higher temperature (95 °C) for 15 min the lytic activity of
Lytic activity of a 1:10 diluted supernatant was detected in all samples stored for 3 days at various pH ranging from 2 to 12. Maximum lytic activity was observed at pH 8 and lytic activity decreased with higher and lower pH, but did not disappear completely (
Liquid-liquid partitioning between aqueous supernatant and
When the supernatant was pre-acidified to pH 2, and then extracted with
When using extraction solvents with intermediate polarity, both dichloromethane-methanol and chloroform-methanol, did extract components with lytic activity from the aqueous phase. With dichloromethane-methanol mixtures, little but significant activity was still detected in the aqueous phase (
Attempts to concentrate lytic activity by taking up methanolic extracts in less volume than the original supernatant were partially successful. When compared to untreated supernatant, methanol extraction and re-suspension in the same volume yielded > 40% loss of activity (
The mass range of the lytic compounds was estimated via ultrafiltration with 5 kDa and 500 kDa cut-off membranes. When small volumes of supernatant (
However, when 100 mL supernatant were passed through a 500 kDa membrane, activity was found in the initial filtrate and increased in filtrates of subsequent runs (
When 5 mL of concentrated supernatant (extracted from 50 mL supernatant by chloroform-methanol (3:1) and concentrated to 5 mL) were applied to a Superdex 30 gel permeation column for size determination, lytic activity was primarily found in Fraction 3 and to a lesser extent in Fraction 4 (
Initial clean-up experiments on the cell-free culture supernatant by reversed phase SPE C-18 cartridge yielded no apparent mortality of
Yet 100% mortality of
Lytic activity appeared in the sample load eluate only after 40 mL supernatant was applied (
Storage of lytic active eluates from reversed phase SPE in seawater-base culture medium yielded markedly different effects, depending upon the specific storage conditions. Re-suspension of dried SPE fraction in seawater based K-medium resulted in higher lytic activity (lowest EC50 value,
Reversed phase chromatography of SPE pre-cleaned supernatant (80% methanol eluate) on a lipophilic C8 phase yielded an active fraction between 18 and 19 min. None of the other fractions collected in one minute increments showed any lytic activity in the
The EC50 was reached with 1.9 mg (1.7–2.1 mg) of dried supernatant. After clean-up by reversed phase SPE, the EC50 was equivalent to 78 μg (60–101 μg) of the 80% methanol fraction. The same activity was achieved with 31 μg (28–34 μg) of the lytic HPLC fraction (18–19 min). These results indicated that 96% of interfering substances in the supernatant were removed by the SPE clean-up. Salt removal from the supernatant mainly contributes to this reduction of dry mass equivalent. Further separation by C8 HPLC additionally excluded approximately another 60% of dry mass not related to lytic activity.
Species of the genus
The successful search for allelochemicals is also highly dependent upon the appropriate choice of bioassay to follow biological activity. Rather than the commonly used brine shrimp or mammalian cell lines, we selected the small cryptophyte
Previous attempts to purify and isolate these allelochemical compounds by classical bioassay-driven fractionation often resulted in loss of activity and erratic and irreproducible results. For this reason we decided to carefully characterize the properties and behavior of these compounds before starting their isolation. As a prerequisite for effective bioassay-guided fractionation it is necessary to obtain basic information on physico-chemical properties of the target compounds. The stability of the compounds, or more precisely the stability of the biological effect quantified with the bioassay is important. For example, toxic compounds of the haptophyte
Perhaps surprisingly given the complexity of the probable macromolecular structures lyophilization of supernatant hardly altered lytic activity after resuspension in culture medium. Yet the lytic effect of cell-free supernatant appeared to rapidly decrease (
Amphipathic compounds, such as detergents and surfactants, are generally large molecules composed of an ionic or non-ionic polar head and a hydrophobic part, which promote the formation of aggregates (micelles, bilayers, monolayers, hexagonal or cubic phase) in aqueous media. Most important, physico-chemical behavior and properties of amphipathic compounds are strongly modulated by self-association or by their binding to other lipophilic aggregates. Monomers and aggregates can be interconverted as a function of pH, temperature, ionic strength, and concentration of the compounds (for a review, [
Bioactive compounds from other toxic marine flagellates, including species of
Fatty acids have been reported to be associated with hemolytic, antibacterial or anti-algal effects in several dinoflagellate species [
The results further show that, although temporal stability of the compounds is high, substantial losses seem to be unavoidable during purification. This may be generally explained by a "stickiness" of the compounds to various surfaces, as has been described for filter material [
It is also interesting to consider how storage conditions affected lytic activity. Pretreatment of the partly purified lytic fraction in seawater – compared to ion-free water or different solvents - increased the activity (
Apparently, two distinct lytic fractions with different polarity (high polarity: eluted with 20% methanol; low polarity: eluted with 80% methanol) were separated via reverse SPE (
Nevertheless, more than 95% of total activity of the supernatant applied to SPE could not be eluted from the column. It is unlikely that this loss was caused by nullifying synergisms of two different compounds now separated into two different fractions, as simple mixing of the two fractions did not significantly increase the lytic activity. A strong adsorption of the non-polar fraction to C-18 material, as has been found for all kind of surfaces, might be responsible for the large loss.
The fact that lytic activity elutes in a discrete retention window between 18 and 19 minutes from a reversed C8 phase but does not show any specific absorption of UV radiation, may be explained by two alternative hypotheses: 1) lytic compounds are active at very low concentrations and coelute with compounds unrelated to lytic activity on this chromatographic system, or 2) lytic compounds belong to a suite of chemically similar (but non-lytic) compounds and only small modifications, which do not result in altered UV absorption, are responsible for lytic activity. Both hypotheses will have to be tested in future work.
A still unresolved problem is the loss of activity after purification and separation of supernatant. Similar effects have been described for karlotoxins produced by
There are three other recent studies dealing with exotoxins of the genus
One clonal isolate of
Different techniques for maximum yield of extracellular lytic activity from
Water was deionized and purified (Milli-Q, Millipore GmbH, Eschborn, Germany) to 18 MV cm−1 quality or better. Methanol (LiChrosolve for HPLC, and p.a. for all other experiment), chloroform (p.a.), dichloromethane (p.a.), hydrochloric acid (HCl, 32%, p.a.) and
A microalgal bioassay system was used to quantify lytic activity based on the response of the photosynthetic cryptophyte
The bioassay was performed in two different ways. For the "one-point" approach to even detect a minor decline of lytic activity, one concentration of sample was applied and percent mortality (by comparing cell number to a control) were calculated as a measure of lytic activity of the sample, and in these experiments, the supernatant was properly diluted before the experiment to have a start activity of the compounds close to 100% target mortality. For detailed quantification, a dose response curve was determined, which allows for a calculation of EC50 concentration,
In one-point bioassays, mortality of
When performing dilution series experiments, the percentage of intact
For one-point measurements,
Stability of lytic activity under normal culture incubation conditions was tested by filling 24 glass vials (Wheaton, 20 mL) each with 15 mL of 1:10 diluted (with K-medium) supernatant and following the decline in activity over time with a one-point bioassay. Incubated samples were stored in a culture room at 15 °C for 1, 4, 7 and 14 days, respectively. After each respective incubation period, six vials were removed; 3.9 mL sub-samples were taken without perturbation from three vials, whereas the other subset of three vials was shaken vigorously before sub-sampling. A
A fully quantitative estimation of stability under normal culture conditions was then performed as follows: 500 mL of supernatant was prepared and apportioned into 5 × 100 mL in 100 mL Duran glass flasks. From one flask, lytic activity was quantified from an 11 points dilution curve (each dilution in triplicate). The other flasks (in duplicates) were stored under normal dinoflagellate culture conditions (as described herein). One of the two sets of flasks was kept dark by wrapping with aluminum foil. After 1, 2, 4, 7, 12, 20 or 49 days, sub-samples of each flask were taken after vigorously shaking the flasks to perform a bioassay and the activity was determined from an 11-point dilution curve (each dilution in duplicate). For the final sampling (after 49 days), samples from flasks held in the light had to be omitted because a low but substantial number of cells was present, which had obviously originated from a few cells still present in the culture supernatant. After 7 days, 1 mL sub-samples from each flask were fixed with formalin (2% final concentration) for determining bacterial abundance according to a standard technique with acridine orange staining [
Effects of freezing were tested by a quantitative comparison of lytic activity of supernatant before and after prolonged (3 month) storage at −20 °C in the dark. A total of 200 mL supernatant was prepared and a standard bioassay to quantify lytic activity was performed (in triplicate) with a series of 9 dilutions. Subsequently, an aliquot of 50 mL of the remaining supernatant was stored in the freezer at −20 °C in a glass bottle. After 3 months storage, the sample was thawed at room temperature and lytic activity was quantified from a 9-point dilution curve (in triplicate).
Frozen supernatant (90 mL at −20 °C) was lyophilized by a Freeze Dryer Beta I (Martin Christ, Osterode am Harz, Germany) at −20 °C and ca. 0.004 mbar vacuum. After three days, the lyophilized sample was re-dissolved in 90 mL water, and lytic activity was quantified by an 8-point dilution series bioassay and compared to untreated supernatant, which was stored at 4 °C during lyophilization.
Short-term heat stability was tested by incubating 20 mL supernatant in 20 mL glass vials in duplicate at room temperature (ca. 20 °C) as control, and at 60 and 95 °C, each in a water bath for 15 min, respectively. The heated samples were rapidly cooled with ice to room temperature. From each sample, a
The effect of storage at different pH on extracellular lytic compounds was tested as follows. Fifteen mL of 1:10 diluted supernatant in duplicate were stored in 20 mL glass vials at pH ranging from 2 to 12. The pH was adjusted with NaOH (4 M and 0.1 M) and HCl (32% and 3.2%). Samples were stored for 3 days in the dark at 15 °C, and then adjusted with NaOH and HCl to pH 8. The increase in volume due to this adjustment was always below 1 mL. The
Partitioning of lytic activity after solvent extraction was analyzed as a first step towards a chemical characterization. The prime task was to exclude the possible negative effect of
Fifteen mL Alex2 as well as Alex5 supernatant in triplicates were acidified with 1 and 0.1 N HCl to pH 2.0. Then the samples were extracted three times with 5 mL
Chloroform, dichloromethane, methanol and mixtures of these solvents, which are less hydrophobic, were tested in different ratios (chloroform or dichloromethane: methanol = 3:1; 2:1; 1:1 (v/v)). Twenty mL supernatant were mixed with 5 mL of each solvent combination, and subsequently centrifuged (3,220 × g, 5 min, 15 °C). Organic phases were carefully removed from the bottom with a pipette. The aqueous phase was extracted twice more with 5 mL each of the respective organic solvent, and then dried by rotary evaporation and dissolved in 20 mL water. The organic phases were combined and dried by rotary evaporation and finally dissolved in 20 mL K-medium. Lytic activity of both, organic and aqueous phases was measured by
To concentrate lytic activity for further analysis, 30 mL supernatant were extracted three times with 5 mL chloroform/methanol (3:1 (v/v)) in triplicate. After evaporation to dryness, the residue of the organic phase was taken up in 3, 6, 15, 30 mL K-medium, respectively, to obtain a calculated 10, 5, 2, and 1-fold concentrated supernatant. Lytic activity was quantified with a 7-point dilution series.
Ultrafiltration was performed with an ULTRAN®-MiniFlex cross flow system (Schleicher & Schuell MicroScience, Dassel, Germany) with a filter area of 24 cm2 per cassette, operated at a pressure between 1.0 to 1.8 bar. Ultrafiltration membranes PES 500 (cut-off 500 kD) and PES 5 (cut-off 5 kDa) (Schleicher & Schuell MicroScience, Dassel, Germany) were used. For the 500 kDa membrane, 200 mL supernatant were applied until the retentate reservoir was empty. The filtrate was collected in two separate aliquots of 100 mL. Lytic activity of these two filtrate fractions was tested by taking 3.9 mL sub-samples (in duplicate) for a one-point
Fifty mL of supernatant were extracted with chloroform/methanol [3:1 (v/v)] as described above, and the residue of the rotary-evaporated organic phase was dissolved in 5 mL water. The concentrated sample was applied to a Superdex 30 GPC column (Hiload 16/60, 13,000 plates), with 40% methanol as eluent. Each run was performed for 120 min at a flow of 1.15 mL per min. For each run, 12 fractions were collected (10 min for each tube), and rotary-evaporated to dryness. The residues were dissolved in 5 mL K-medium and incubated for two days. A one-point (3.9 mL) bioassay was performed as described above to determine lytic activity in each fraction, together with supernatant of the same batch as positive control. Subsequently, 150 mL supernatant was concentrated the same way to 15 mL, and was applied to the GPC column by injection in three subsequent portions. Corresponding fractions from all three runs were pooled and rotary-evaporated to dryness, and finally the lytic activity as well as supernatant of the same batch was tested via bioassay with a 7-point dilution series.
Lytic compounds were extracted from the supernatant by reversed-phase SPE cartridge (LC-18, 500 mg/6mL, Sigma-Aldrich, Deisenhofen, Germany). The cartridges were conditioned by 15 mL chloroform/methanol (3:1 (v/v)) and 15 mL 100% methanol and equilibrated with 15 mL water. First, in order to define a minimum volume of supernatant to be applied to SPE, a series of four volumes (10, 50, 100, and 200 mL) of supernatant were applied to SPE cartridges and each eluted with 10 mL water, 40% methanol, 100% methanol, and chloroform/methanol (3:1 (v/v)). Based on the preliminary test, 100% methanol fractions were targeted, and dried by rotary-evaporation, re-dissolved in 5 mL K-medium and tested as a one-point bioassay of undiluted sample for lytic activity. In the next experiments, 200 mL supernatant in triplicate as well as 200 mL K-medium serving as negative control were applied to the cartridges, and eluted with 10 mL water twice and subsequently with 20 mL of 20, 30, 40, 50, 60, 70, 80, 90, and 100% methanol, and finally with 10 mL chloroform/methanol [3:1 (v/v)]. Each eluate fraction except for first 10 mL sample load eluate (salt included) was dried by rotary evaporation and re-dissolved in 5 mL K-medium. Sub-samples of 3.9 mL each as well as the waste from the last 5 mL (195 to 200 mL) were tested as a one-point bioassay. Based on the results of the preliminary tests, the final SPE elution protocol is as follows: after sample (200 mL supernatant) loading the cartridge was washed with 10 mL water, 10 mL 20% methanol, 20 mL 50% methanol and 10 mL 80% methanol.
In order to quantify the lytic potency of two lytic fractions eluted with diverse eluents gained from SPE, 200 mL supernatant was applied to one C-18 cartridge, and the eluates were collected every 20 mL. For the first 5 eluate collections, one-point (3.9 mL) bioassays were performed in duplicates, while a 4-point dilution curve (3.9, 2.0, 1.0, and 0.5 mL) was performed with last five eluate collections in duplicate. The loaded SPE column was eluted as the final protocol.
The 10 mL water and the 10 mL 20% methanol fractions were combined and dried by rotary evaporation, and then were re-dissolved in 20 mL K-medium. To this H2O-20% methanol sample, a 5-point dilution series was performed including a negative control (K-medium) to exclude possible salinity effect on
Lytic effects of SPE fractions maintained under different storage conditions prior to applying the bioassay were investigated by applying 800 mL supernatant to 4 SPE cartridges (200 mL each) and eluted as described before. All 80% methanol fractions were pooled and split into 4 sub-samples (10 mL each). One sub-sample was stored in 80% methanol (1) in the fridge at 4 °C. All other sub-samples were dried by rotary evaporation. One fraction was stored as dried sample (2), another resuspended in 5 mL water (3) and the last sample (4) re-suspended in 5 mL K-medium. After 2 days, aliquots (1) was rotary evaporated to dryness and then all aliquots were re-suspended in 10 mL K-medium. Immediately, a first bioassay was performed for each sample with a 6-point dilution curve in duplicate. All samples in culture medium were then stored in the fridge at 4 °C for an additional two days; thereafter new bioassays were performed with the same dilution series.
HPLC was performed using an Agilent 1100 series (Agilent Technologies,Waldbronn, Germany) system. The LC-system consisted of a G1379A degasser, a G1311A quaternary pump, a G1229A autosampler, a G1330B autosampler thermostat, a G1316A column thermostat and a G1315B Diode-array detector (DAD). Chromatographic conditions were as follows: mobile phase A: water; mobile phase B: methanol. The flow rate was 0.2 mL/min with the following gradient: 0 until 15 min, 5% to 100% B; isocratic 100% B until 27 min; 27 until 28 min, 100% to 5% B; isocratic 5% B until 40 min (= total run time). The autosampler temperature was set at 25 °C and the injection volume was 100 μL. One liter supernatant of both Alex2 and Alex5 cultures was cleaned up by C18 SPE (500 mg/6mL, Sigma-Aldrich, Deisenhofen, Germany). The 80% methanol fraction was collected, dried by rotary evaporation, and finally dissolved in 1 mL water. Before injection, the sample was spin-filtered (Eppendorf 5415R, 30 sec, 15,000 × g, room temperature) by filter unit insert (0.45 μm, Durapore, Millipore). The separation of analytes was performed on a 50 × 2.0 mm i.d., 3 μm, Hypersil BOS C8 reversed-phase column (Phenomenex, Aschaffenburg, Germany) equipped with a Phenomenex SecuriGuard pre-column. Based on preliminary result, eluate was collected very 1 min from 15 to 20 min, and lytic activity was detected in each fraction. Accordingly, the target range was detected over the wave length range 190–400 nm.
For the determination the dry mass equivalent of lytic activity, bioassays with dilution series were performed with supernatant, the lytic SPE fraction and the lytic HPLC fraction (details see above experiments). Based on the EC50 value, the corresponding volumes of the treatments were calculated. These respective treatment volumes were dried in a N2 stream and dry residues were weighed.
Analysis of physico/chemical characteristics of extracellular lytic compounds produced by
Funding was provided to Haiyan Ma by the Deutscher Akademischer Austausch Dienst through a DAAD-Helmholtz scholarship. Financial funding was provided by the PACES research program of the Alfred Wegener Institute as part of the Helmholtz Foundation initiative in Earth and Environment. We would also like to thank three anonymous reviewers and the editor who provided constructive comments that improved this manuscript
Mortality of
Stability of lytic activity of supernatant after storage under culture conditions. (A) Initial dilution curve of supernatant. Intact
Intact cells of
Mortality of
Intact cells of
Intact cells of
Mortality of
Intact cells of
Mortality of
Mortality of
Intact cells of
Intact cells of
EC50 (sample %) and 95% confidence intervals for SPE lytic fraction dissolved and stored in four different ways.
|80% methanol
|3.4 (2.8–4.2)
|2.8 (2.7–2.9)
|22.2 (19.4–25.4)
|5.0 (4.3–5.8)
|2.1 (1.9–2.3)
|2.3 (1.9–2.8)
|4.1 (3.5–4.7)
|2.6 (2.6–2.7)


text: Hi folks. I'm having issues with transparency on my custom shaders. The set up I have is two quad-spheres at the same position but one larger than the other. The shader for the inner/smaller quad-sphere is set to render during the geometry queue and is opaque, and the shader for the outer/larger quad-sphere is set to render during the transparency queue and has alpha blending on. The issue is that parts of the outer sphere are obscured by parts of the inner sphere incorrectly and thus it doesn't render completely. I've been messing around with z-write settings but no combination seems to fix the problem. You can see a screen grab of the issue here: Does anyone know what could be causing this and how I could go about fixing it? Cheers folks


text:
Why Jason Newsted Left Metallica [VIDEO]
Jason Newsted, the former bass player for Metallica has broken a 13-year silence in a recent interview as to the real reasons behind his departure from the legendary band. In an interview with ScuzzTV, Jason Newsted opens up about what was behind his decision to leave Metallica.
Apparently the decision stemmed from a side project you may remember Newsted being a part of a band called 'Echobrain.' The fact that James Hetfield and Metallica wouldn't support him on it ultimately led to Newsted's decision to leave.
Listen to Newsted's entire interview and the full story below.
Subscribe to 105.7 The Hawk on
For those that may or may not remember Echobrain, you can listen to one of their first songs with Jason Newsted, called "Spoonfed" below.
Subscribe to 105.7 The Hawk on


text:
Bye Bye Milan Fly
For some reason I always come across flyswatters. Does that mean am I compulsive. But I know one thing. I'm very stylish swatting flies with my new Milan streetscape flyswatter.
Choose any color candle and let it drip/melt down the bald head creating waxy eerie locks.Maybe a strange valentine gift!?lol!Tags: home, gifts, baby, furniture, lighting, accessories, decorate, dolls, home decorAdded 9 Years Ago from Notcot


text:
Fill in a Spreadsheet with Data
Költségvetés $250-750 USD
Hello, I am a part of a small fitness site and I would like a list of all my member's emails so I can start sending out a newsletter. You will have to log into the account, click each member's profile and then copy their email address into an xcel spreadsheet.
We have about 11,000 members and it takes about 10 minutes to do 70 members.
82 szabadúszó tett, átlagosan $396 összegű árajánlatot erre a projektre.
With respect to this project I would like to present myself as a candidate for your consideration. I have more than 7 years of IT experience. I have successfully completed few projects for US clients Relevant Skills a Bővebben
Hi There I've read your brief and can see that you'd like to do copy email from members profile Relevant Skills and Experience I have 6 years of experience in data entry, web research, Data Processing, collect detail Bővebben
Even if I am just a newbie, I know that my skills and dedication to work will be my keys in order to have a good quality result of any task given to me. Hope you will consider my application. Relevant Skills and Exper Bővebben
I'm an experienced Excel user, and have been working with smart tools and analysis for already 10 years. I have finished a similar job recently. Relevant Skills and Experience Data organization Advanced Formulas Pivot Bővebben
Hi, I would like to bid for this project. I have a wide experience relevant to data entry and typing job. If given a chance to be part of this project i can assure you a 100% satisfaction. Thank you Relevant Skills Bővebben
I will complete the work on time Relevant Skills and Experience I have organizational experience in specified skill Proposed Milestones $555 USD - Assigned work
. Relevant Skills and Experience . Proposed Milestones $250 USD - .
Hi My name is Daniel Giles, I Am from south africa and I'm new to Freelancer i will give you the Best possible Job i can Do and ill prove it to you. please give me this opportunity. kind regards daniel


text:
MEMBER LOGIN | REGISTER | Forgot Password
C Spire Wireless (Ridgeland, Miss., U.S.A.), a privately held regional U.S. mobile phone network operator, will become the fourth U.S. operator to sell the Apple Inc (Cupertino, Calif., U.S.A.) iPhone in "coming weeks" beating much bigger rival T-Mobile USA to a Apple deal.
C Spire, which recently changed its name from Cellular South, has about a million customers in four states, making it more than 33 times smaller than No. 4 U.S. operator T-Mobile USA, which has 33.6 million customers.
C Spire said the device would be for bill-paying subscribers rather than pay-as-you-go customers, but declined to give further details about its deal with Apple.
T-Mobile USA, a unit of Deutsche Telekom , said in September that it has told Apple it would like to sell the iPhone and referred additional questions to Apple.
No. 3 U.S. mobile provider Sprint Nextel became the third U.S. operator to offer iPhone earlier this month, after AT&T Inc and Verizon Wireless, a venture of Verizon Communications and Vodafone Group Plc. T-Mobile USA is fighting in court for regulatory approval to be bought by AT&T in a $39 billion deal that U.S. regulators have sought to block.
(Reporting by Sinead Carew)
Related Articles
Sprint confirms iPhone, concerns linger
Samsung considers blocking release of new Apple iPhone
Communications 2020
July 18 - 20, 2017
Las Vegas, NV
M2M Zone Pavilion @ MWC Americas 2017
September 12-14, 2017
San Francisco, Calif.


text:
Chart of the Day - 9/09/2009 – EUR/USD
(Chart courtesy of FX Solutions' FX AccuCharts. Price on 1st pane, Slow Stochastics on 2nd pane; horizontal support/resistance levels in yellow; uptrend lines in green; downtrend lines in red; 50-period simple moving average in light blue.)
9/09/2009 – EUR/USD – After breaking out yesterday above the last major high of 1.4445 within the current uptrend, price action on EUR/USD has hit its initial upside resistance target at the top of a parallel uptrend channel that the pair has been traversing since early June. In the process, this currency pair has confirmed a continuation of the current prevailing uptrend and has made a new 2009, or 8-month, high. The next key upside target resides right above 1.4700, which represents the top of the spike that occurred at the end of 2008. Any near-term breakout above that price region would reach at least an 11-month high, and could subsequently target further key resistance in the 1.4900 price region. Downside support within the context of the current uptrend continues to reside around the bottom border of the major parallel uptrend channel that has been in place since early March.
James Chen, CMT
Chief Technical Strategist
FX Solutions
IMPORTANT NOTICE: These comments are for information purposes only. The information contained on this document does not constitute a solicitation to buy or sell by FX Solutions, LLC., and/or its affiliates, and is not to be available to individuals in a jurisdiction where such availability would be contrary to local regulation or law. Opinions, market data, and recommendations are subject to change at any time. Forex trading involves substantial risk of loss and is not suitable for all investors.


text:
SEBRING -- Sebring only needed six innings to blank Jackson-Milton 10-0 in a Division IV sectional baseball tournament semifinal at Akenhead Field.
Junior Josh Hurford fired a one-hitter for the Trojans, yielding a two-out single in the fifth inning.
Sebring (15-7), which split the two regular-season contests with Jackson-Milton, has won eight of its last nine games.
"We're playing loose," Sebring head coach Doug Eaton said. "We were patient, we swung at good pitches and we played well in the field."
Hurford was efficient, firing 77 pitches, including 55 for strikes. He walked one and struck out six. Only baserunner reached second base.
Sebring backed Hurford with eight hits. Hurford helped his own cause with two singles and two RBI. Mitch Baddeley added two singles and two runs batted in.
Dakota Wagner only had one hit, but it was double to right field in the last of the sixth inning which chased home two runners to end the game.
Jerry Billingsley added two singles.
Sebring scored its first four runs in the first inning without benefit of a hit. The first four batters walked, capped by a free pass to Jake Hubbs with the bases loaded. Wagner knocked in a run with a fielder's choice, Corey Baia made it 3-0 with a sacrifice fly and the fourth run scored on a passed ball.
The Trojans advanced to the sectional final to meet the McDonald Blue Devils. The two teams are scheduled to play at McDonald this afternoon at 4:30.
Jackson-Milton 000 000 -- 0 1 1
Sebring 401 203 -- 10 8 0
Hurford and Arbitter. R. Nagy, Totanni (1), Fitzpatrick (2) and Anderson. WP -- Hurford (7-1). LP -- R. Nagy. 2B -- Wagner. 3B -- None. HR -- None.
A five-run fifth inning propelled the Canton South Wildcats (17-6, 11-2) past the Alliance Aviators (15-7, 9-4) 6-2 in a Northeastern Buckeye Conference regular-season game played at Craven Field Wednesday.
Nathan Rice walloped a double to highlight the Flyers' batting. Conner Thompson belted a double to pace the Wildcats.
Wildcat starting pitcher Joe Costello garnered the win. Luke Ince was the losing pitcher for the Aviators.
The two squads play each other again today at the Wildcats' home field.
Canton South 001 050 0 -- 6 10 3
Alliance 000 002 0 -- 2 6 4
Joe Costello, Kendall Fowler (6), and Conner Thompson. Luke Ince, Jacob Turney (6), and Alex Bugara. WP -- Costello (4-1). LP -- Ince (4-2). 2B -- Thompson (CS), Rice (ALL). 3B -- None. HR -- None
LOUISVILLE -- St. Thomas Aquinas edged Northwest 7-6 in a regular-season game at Bagnola Field.
The Knights (6-15) scored five runs in the sixth inning, overcoming a 6-2 deficit.
Freshman Frank DiMarzio ignited the inning with a two-run single. C.J. Olszewski added a run-scoring single. The fourth and fifth runs scored on a throwing error.
DiMarzio and junior Joe Blevins both singled twice for the Knights.
Northwest 212 010 0 -- 6 10 2
St. Thomas Aquinas 100 105 -- 7 8 3
Crihfield, Earnsberger (6) and Heitger. Moeglin, Burchett (5) and Palutsis. WP -- Burchett (1-2). LP -- Crihfield. 2B -- Pusateri, Borotkanics (N), Earnsberger (N). 3B -- None. HR -- None.
BELOIT -- The Minerva Lions overcame a 3-1 deficit to get by the West Branch Warriors in Northeastern Buckeye Conference baseball action Wednesday afternoon.
Minerva, 6-7 in the NBC, pounded out 14 hits and was led offensively by Zach Wade who was two-for-five with a pair of runs scored and one RBI, Tyler Klimko, with two singles in five at-bats and one run batted in, Keanan Locke who whacked two hits in three at-bats with one RBI, Chase Petree who also went two-for-three including a double and an RBI, and Tito Ramos who finished with two hits in four at-bats.
The top hitters for West Branch (14-11, 7-6) were Brenden Wells who had one double and one triple in three at-bats and scored one run, Beau Taylor who was two-for-three with a double, and Jeff Vizzuso, who smacked a double in his three at-bats with two runs batted in.
Petree garnered the pitching win. Nate Coy took the tough loss.
Minerva 100 201 1 -- 5 14 2
West Branch 021 000 0 -- 3 5 3
Petree and Gould; Mull, Taylor (2), Coy (5), Unkefer (7), and Smith, Ruggles (5) WP -- Petree LP -- Coy (0-1) 2B -- Petree (MIN), Wells (WB), Taylor (WB), Vizzuso (WB) 3B -- Wells (WB) HR -- None


text:
News Release
For Immediate Release
May 11, 2012
Susan G. Morrell, Senior Assistant Attorney General
Michael S. Lewis, Assistant Attorney General
(603) 271-3671
New Hampshire Attorney General Michael A. Delaney announces that the investigation has been completed in connection with the officer involved shooting incident that took place in Keene, New Hampshire on Tuesday night, April 17, 2012.
Final Report on Officer Involved Shooting in Keene, NH on April 17, 2012
Adobe Acrobat Reader format. You can download a free reader from Adobe.
New Hampshire Department of Justice | 33 Capitol Street | Concord, NH | 03301
Telephone: 603-271-3658


text:
Police are appealing for witnesses after a man was assaulted in Andover.
The incident took place at the back of the "Club of Life" in London Street between half past midnight and 1am on Saturday.
A 28 year old local man intervened in an altercation outside the club. He then got hit in the mouth with a bottle.
The woman who hit him is described as in her 30s with long dark hair.
PC Jenny Carr at Andover would like to hear from any witnesses, contact her on 101 or via Crimestoppers anonymously on 0800 555 111.


text:
Playing much better than in season-opening losses, Woodlawn and H.L. Bourgeois both registered convincing wins in the second day of the Woodlawn Shootout on Tuesday at WHS.
Woodlawn dominated St. Helena Central 64-30 and H.L. Bourgeois defeated Belaire 63-44. Helen Cox defeated Woodlawn 40-37 and Tara edged H.L. Bourgeois 55-52 in Monday action.
"We were totally out of rhythm last night, but played much better tonight," Woodlawn coach Elmo Fernandez said. "We wanted to speed the game up and play fast. We were able to force turnovers and run."
Woodlawn (1-1) jumped to a 9-0 lead as 5-foot-6 junior guard Andrew Migaud scored five points and had an assist. St. Helena (1-2) got a three-point play from 6-foot-5 senior Johnathan Butler with 4:13 remaining in the second quarter to pull within 18-9.
Woodlawn went on an 11-0 run, capped by a steal and monster dunk by Tyree Spurlock. Spurlock was whistled for a technical on the play and Butler hit one of two free throws as Woodlawn led 29-10. Migaud hit a long two-pointer as the Panthers led 35-15 at the half.
Migaud scored 13 of his 16 points in the first half. Chris Williams added 16, including 10 in the first half for WHS. Spurlock had eight and Cameron Lazare six.
"We have no seniors on the team," Fernandez said. "We should get a couple of guys back next week from injury. I think we could be good down the road. We're still looking for chemistry. Migaud is a good shooter for us, and he has the green light whenever he's open."
Butler led St. Helena Central with 15 points and Brandon Williams added eight. Woodlawn's defense forced 20 turnovers.
"We don't have a point guard right now," St. Helena Central coach Terry Guy Sr. said. "We did too much dribbling and not enough passing. Woodlawn has an excellent team and looked much better tonight than last night. We've got some guys out and think we'll be alright come district and playoff time."
H.L. Bourgeois led Belaire 27-13 at the half. The Bengals pulled within 38-29 after three quarters, but were outscored 25-15 in the fourth quarter.
HLB (1-1) got balanced scoring from Ike Aldridge (17), Ocean Jasper (13), Marlin Bonvillain (12) and Devante Dickerson (9). V. Augustus scored 12 to pace Belaire (1-1).
"We matched Belaire's intensity and that was something that was missing last night against Tara," H.L. Bourgeois coach Andrew Caillouet said. "We played much better defense. We are a small team, but we really battled on the boards. We're a quick team and shoot the ball well. I like how we share the ball."
The Woodlawn Shootout concludes with three games Wednesday. St. Helena meets Tara at 4:30 p.m. and Belaire faces Helen Cox at 6 p.m. Woodlawn battles H.L. Bourgeois in the 7:30 p.m. finale.
"H.L. Bourgeois is talented and it should be a good game," Fernandez said.


text:
Mormon Studies Review adds new associate editor
Melissa Wei-Tsing Inouye is joining the Mormon Studies Review editorial team as an associate editor alongside Morgan Davis and Benjamin Park. Review editor Spencer Fluhman invited Inouye especially in order to broaden the Review's reach in international studies. "We're thrilled to have Dr. Inouye join our editorial staff," Fluhman said. "She brings world class training, a […]Read More


text:
|Document Type:
|Kevin David Dell
|Email address:
|ANTIOXIDANT INTERVENTION WITH MANGANESE(III)-SALOPHEN IN THE SELENITE CATARACT MODEL Implications For Cataract Disease
|Doctor of Philosophy
|Committee Chair:
|John L. Hess
|Chair's email:
|Committee Members:
|Eugene M. Gregory
|David R. Bevan
|Steven D. Holladay
|Hara P. Misra
|selenite, cataract, Mn-salophen, antioxidant, crystallins
|Date of defense:
|May 4, 1998
|Release the entire work for Virginia Tech access only.
After one year release worldwide only with written permission of the student and the advisory committee chair.
Cataract disease affects millions of people worldwide. It is characterized by the accumulation of light-scattering bodies within the lens that reduce visual acuity. Cataracts are effectively treated surgically, but at great expense, costing Medicare $3.4 billion in 1997. Development of an alternative therapy for this disease would provide medical and economic benefits. We have investigated a novel antioxidant, the superoxide scavenger Mn(III)-salophen, as a therapeutic agent in the selenite cataract model. Mn(III)-salophen has been shown to protect E. coli colonies against oxidative stress but was untested in a eukaryotic system. A total dose of 300 µmol/kg, given IP in four equal 75 µmol/kg doses spaced four hours apart, protects 75% of neonatal rats from nuclear cataract development five days after selenite injection. Selenite is toxic through its reaction with the endogenous antioxidant glutathione (GSH). The reduction of selenite to selenide through an intermediate, selenodiglutathione (GSSeSG) leads to generation of superoxide radical, one of several toxic oxygen species that can damage the lens. Mn(III)-salophen causes an in vitro preservation of the lifetime of GSSeSG by interrupting the reduction of selenite. We have established that the reduction of GSSeSG to selenide does not use GSH as a reducing agent, but rather depends upon electrons generated in the earlier reduction of selenite to selenodiglutathione. These electrons can be intercepted by known one-electron scavengers, arresting the metabolism of GSSeSG. Extensive proteolysis of lens crystallins and loss of calcium homeostasis occur in cataractous lenses from a rat treated with sodium selenite. The visual protection with Mn(III)-salophen is accompanied by a partial loss of the calcium homeostasis, a net increase in sodium, and calpain-mediated proteolysis of ß-crystallins similar to lenses from animals treated with selenite alone. Although preservation of alpha-crystallins may contribute to the greater transparency in the protected lens, generalized ß-crystallin proteolysis is insufficient for cataract formation. From these experiments we propose that Mn(III)-salophen minimizes the oxidative stress imposed upon the cell by interfering with the metabolism of selenodiglutathione. This allows the cell to compensate for the loss of cation homeostasis and prevents aggregation of proteolyzed crystallins into cataracts.
List of Attached Files
|At the author's request, all materials (PDF files, images, etc.) associated with this ETD are accessible from the Virginia Tech network only.


text:
Appendectomy—Open Surgery
An appendectomy is the removal of the appendix. The appendix is a pouch that is attached to the large intestine.
Reasons for Procedure
An appendectomy is often done as an emergency procedure to treat appendicitis . Appendicitis is inflammation of the appendix. It can be caused by an infection or obstruction.
|Inflamed Appendix
|Copyright © Nucleus Medical Media, Inc.
Possible Complications
Problems from the procedure are rare, but all procedures have some risk. Your doctor will review potential problems, like:
- Bleeding
- Infection
- Damage to other organs
- Reaction to anesthesia
Complications are more common in people older than 65 years of age. Some risk factors that make complications more likely include:
What to Expect
Prior to Procedure
Your doctor may do the following:
- Physical exam
- Blood and urine tests
- Your doctor may need detailed pictures of your appendix. These can be made with:
Antibiotics will be started right away. Appendicitis is an emergency condition. Surgery is almost always done right away.
General anesthesia will be used. You will be asleep during the procedure.
Description of the Procedure
A short incision will be made in the right lower abdomen. The appendix can be viewed through this incision. The appendix will be detached from surrounding tissue. Any bleeding from the blood vessels will be stopped. The appendix will then be tied off and cut out. The incisions will then be closed with stitches or staples.
If the appendix has ruptured, a warm water solution mixed with antibiotics will be used to wash out the inside of the abdomen. A catheter will then be placed to drain any fluid that builds up. Sometimes, with a rupture, the surgeon will only close the muscle layers and leave the skin open. The open skin wound will then be packed with a moist gauze dressing.
After Procedure
The removed tissue is examined by a pathologist.
How Long Will It Take?
1-2 hours
Will It Hurt?
Anesthesia prevents pain during surgery. Pain and discomfort after the procedure can be managed with medications.
Average Hospital Stay
You may be in the hospital for up to 3 days. If you have any problems, you may need to stay longer.
Post-procedure Care
At the Hospital
Right after the procedure, you will be in a recovery room where your blood pressure, pulse, and breathing will be monitored. Recovery may also include:
- Pain medications
- Antibiotics to prevent infection
- Medication to prevent blood clots
- Getting out of bed and moving around within 24 hours of your surgery
If your appendix ruptured, drainage tubes will be removed after a few days.
Your bowels will work more slowly than usual. Chewing gum may help speed the process of your bowel function returning to normal.
At Home
Recovery takes about 4-6 weeks. When you return home:
- Rest and take it easy for 1-2 weeks. Slowly increase activities as approved by your doctor.
- Do not exercise or do heavy lifting for 1 or more weeks as directed by your doctor.
- Follow your doctor's instructions.
Call Your Doctor
Contact your doctor if your recovery is not progressing as expected or you develop complications, such as:
- Signs of infection, including fever and chills
- Increased redness, swelling, increasing pain, excessive bleeding, or discharge at the incision site
- Cough, shortness of breath, chest pain, or severe nausea or vomiting
- Increased abdominal pain
- Lightheadedness or fainting
- Passing blood in the stool
If you think you have an emergency, call for emergency medical services right away.
- Donald Buck, MD
- Reviewed: 11/2015
- Updated: 03/23/2015
Please note, not all procedures included in this resource library are available at Henry Ford Allegiance Health or performed by Henry Ford Allegiance Health physicians.
All EBSCO Publishing proprietary, consumer health and medical information found on this site is accredited by URAC. URAC's Health Web Site Accreditation Program requires compliance with 53 rigorous standards of quality and accountability, verified by independent audits. To send comments or feedback to our Editorial Team regarding the content please email us at email@example.com.
This content is reviewed regularly and is updated when new and relevant evidence is made available. This information is neither intended nor implied to be a substitute for professional medical advice. Always seek the advice of your physician or other qualified health provider prior to starting any new treatment or with questions regarding a medical condition.
Editorial Policy | Privacy Policy | Terms and Conditions | Support
Copyright © 2008 EBSCO Publishing. All rights reserved.


text:
A stroll through South Asheville's newest neighborhood provides the opportunity to enjoy free concerts, a seasonal farmer's market, and a number of popular shops and restaurants.
Online Reservations
Search B&Bs or Cabins & Rentals
Sign up for your free Travel Guide. Your Asheville story begins here.
Asheville Convention and Visitors Bureau
Buncombe County Tourism Development Authority


text:
by Kristen Rosser cross posted from her blog Wordgazer's WordsBe modest.Be beautiful."Don't cause a man to stumble.""Don't let yourself go." … [Read more...]
Christianity and the "Male Gaze"
July 19, 2013 by Suzanne Calulu
Filed Under: "Nike", A Woman's Choice, Biblical Manhood & Womanhood, Big Happy Family Vision, Blaming the victim, Body Image, Boundaries, Bounded Choice, Christian Modesty, Coercive Religious Groups (Cults), Confession, Contentiousness with other Christians, Control, Defrauding, Demonic Influences, Drinking the Koolaid, Elitism, Embracing sexuality, Fear Based Theology, Fear of Hell, Fear tactics, Femininity vs Feminism, Formulaic Religion, Freedom, Fruit of the Spirit, Gender Equality, Group Think, How Did I Get In This Mess?, Issues, Judgementalism, Kobayashi Maru ~ The No-Win Scenario, Legalism, Lusting, Male Headship, Markers of a Cult, Men are Visual, Mental / Emotional Health, Modest Clothing, More from NLQ ..., NLQ Miscellany, NLQ Open Comments, NLQ Quote Worthy, NLQ Stories, Old Paths, Patriarchy, Perfectionism, Personal Autonomy, Purity, Purity Culture, Quiverfull Counterculture, Quiverfull Discernment, Quiverfull Discernment Websites, Quiverfull Topics, Rape Culture, Recovering from Spiritual Abuse, Recovery, Religious Trauma Syndrome, SASBN, Self Esteem, Self-Abnegation / Martydom, Sexuality, Shame, Shunning, Spiritual Abuse, Spiritual Abuse & Recovery, Spiritual Abuse Survivors, Spiritual Issues, Submission, The "World", The Godly Woman, The Spiritual Abuse Survivor Blogs Network, Wholesome Wear Swimsuits, Wordgazers Words - KR Wordgazer - Kristen Rosser Tagged With: Body Image, Defrauding by immodesty, modesty, Sexual Empowerment, Summer Clothing, Swimsuits


text:
In the film that launched the James Bond saga, Agent 007 (Sean Connery) battles mysterious Dr. No, a scientific genius bent on destroying the U.S. space program. As the countdown to disaster begins, Bond must go to Jamaica, where he encounters beautiful Honey Ryder (Ursula Andress), to confront a megalomaniacal villain in his massive island headquarters.
Directed by:
Terence Young
Sean Connery, Ursula Andress, Joseph Wiseman, Jack Lord, Bernard Lee, Zena Marshall


text: High time to present some special sort of Simpsons in shape of porn anime! Simpsons porn erotic drawing characters fuck like rabbits: it's the first time you see them like that lusty and craving sex! Heroes simply cannot quit Marge a scorching new episode of this bizarre sex toon that go mad for each other then for their sexual desires !


text:
Italian divers find wall of bodies in hull of capsized migrant trawler
Deep sea specialists describe horror of finding scores of dead after fiery capsizing of trawler
Deep sea divers have "unpacked a wall of people" from the hull of a trawler on the sea floor near the island of Lampedusa, untangling the dead would-be migrants in the latest and most painstaking phase of a recovery operation following the ship's capsizing.
It was the first time the divers had been able to reach the hull, and authorities said more than 40 bodies were recovered, raising the death toll from last Thursday's tragedy to 274. Scores more are believed missing. Most, if not all, were Eritreans trying to reach Europe in search of asylum and a better life.
"They unpacked a wall of people," said navy captain Paolo Trucco of the deep sea specialists. The bodies "were so entwined, one with the other, it is indescribable. They were so trapped they were difficult to pull out".
Deep sea divers in their weighted suits and sturdy port-holed helmets were able to spend up to 30 minutes at a time at the site of the wreck 47 metres below the surface.
This allowed them to remove debris that was still floating around inside narrow passage ways to reach the vessel's hull. "Mattresses, blankets, stairs. Anything that would float. Imagine if you put a house in a centrifuge and you see what winds up in the air. That is what happened," Trucco said.
Diver Marco Presti said the bodies were packed together when the ship capsized, and he and his colleagues had to pull each out by the arms.
"One diver after the other, we passed them from one to the other, and placed them on the stern of the boat," he said. It was expected to take two more days to complete the search and recovery mission.
Only 155 of the migrants survived. Some have said there were at least 500 people aboard the 18-metre-long smuggler's boat.
Diver Riccardo Nobile, who did multiple dives on Sunday when 83 bodies were recovered in easier to reach areas, said he waited for more than an hour among the corpses on a recovery boat as other divers took their turns. Divers from the navy, coastguard, Carabinieri military police and the fire department have done rotations.
"It was difficult to look straight at their faces, to see their wounds, see their tormented expressions, their outstretched arms," Nobile said. "It was extremely difficult. But this is our job."
The ship had arrived within sight of Lampedusa, a tiny island that is Italy's southernmost point, after two days of sailing from Libya when a fire set to draw the attention of potential rescuers sparked a panic on board, leading the vessel to capsize.
Tens of thousands of migrants from Africa and the Middle East try to cross the Mediterranean Sea each year. Hundreds die in the process.
Most Popular
- 1
- 2
- 3
- 4
- 5


text:
How To Pack Couture
Japan Vogue editor Anna Dello Russo is one of those people you see everywhere at fashion shows around the world, scurrying from show to show and looking fabulous in whatever amazing combination of couture she's wearing. The ladies over at The Cut cornered Dello Russo during New York Fashion Week to find out how one woman packs so many phenomenal, and phenomenally expensive garments, into her suitcase. (How great is Dello Russo's wardrobe? In Milan, where she lives, she keeps a separate apartment just for her clothes.) Her traveling secrets: designer shoes on the bottom, beautiful coats folded on themselves, and, gasp, she wears sweatpants on the plane. Mio dio! [The Cut]


text:
So according to the way the chapter ends, Hashirama was left with a choice of killing himself or his brother so that the world can possibly become peaceful like he dreamed of one day... well here is my theory on what will happen, and it won't be too detailed due to me needing to sleep soon.
I believe he will kill himself for the sake of peace. Now I know that will raise a bunch of questions as to how he was there to do things after the big Madara fight, well here is my answer to that.
What if when he killed himself, Tobirama couldn't handle losing his brother which is what influences him to create the Edo Tensei. He revives his brother Hashirama with the technique (no clue on when, it would possibly have to be a time when Madara was around to see it, because that's the only way he could have known how to stop the technique as well unless that was a fail safe that Kabuto had put in his memory.)
Anyway, that's my theory... hope it doesn't sound too stupid.