text:
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Apr 25, 2014 - 07:02 PM
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(Apr 25, 2014)
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(Apr 25, 2014)
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(Apr 25, 2014)
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(Apr 25, 2014)
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2014 Richmond: Ford NASCAR Sprint Cup Qualifying Quotes
Posted by: ASkyler on Apr 25, 2014 - 07:00 PM
2014 Richmond: Ford NASCAR Sprint Cup Qualifying Quotes
BRAD KESELOWSKI, No. 2 Detroit Genuine Parts Ford Fusion / QUALIFIED 2nd
TALK ABOUT THE PRACTICE SESSION AND QUALIFYING BEING CANCELED TODAY. "We have been keeping the trend up here of starting up toward the front and that is something I am really happy about. Pit road selection is important with that. Always here it is a track position battle but probably more so than ever here this weekend. We did some runs and felt like being in traffic would slow our car down tremendously. Starting up front hopefully we can just avoid it the entire race. The Penske cars have been very strong all season and I am proud of that. My teammate is especially strong this weekend and I think he will be one of the guys to beat. Hopefully we can get right there with him and get a solid race and a win. Richmond hasnât always been the best race track for us so we would like to get the great finish we have been capable of and just havenât put all the way together. We would still like to qualify and have a shot at the pole but we foresaw this coming and put a little extra emphasis in the practice sessions to get a good run out of it and a good spot and I am glad to see that kind of panned out for us."
SINCE YOUR DREAM SCHEDULE BLOG POST HAS CREATED A LOT OF TALK. YOU SAID YOU CANâT GET EVERYTHING YOU HOPED FOR, BUT WHAT KIND OF REACTION ARE YOU GETTING? "You can never expect to get everything you want in anything. In some cases you just hope to get something. I think it is just a great topic that is healthy for our sport to always have a discussion ongoing, whether or not we all agree which is going to be very seldom, few and far between when we can find things where everyone in the sports agrees upon. When I look at my role as an ambassador of the sport, it is one that I take very seriously. Being a part of that dialogue and attempt to always better the sport is one of the things that I would like to play a role in if possible. Certainly I donât have all the answers or pretend to have all the answers. You start with what you think and how you feel and I am glad to share those things with our fans. I donât really see anyone else out there doing that. It kind of puts me in a unique spot and I like to have fun with that and I think our fans do to."
BRAD KESELOWSKI CONTINUED ⦠HAS THERE BEEN ANY REACTION TO IT THAT CHANGED YOUR THINKING? "There are always better ideas. It is hard to think of one specific off the top of my head. I didnât come in here with a list, sorry. I am sure there are always better ideas and I donât pretend to know everything, I just have fun with it."
YOU DESCRIBED YOURSELF AS AN AMBASSADOR OF THE SPORT. IS THAT EVERY DRIVERS JOB OR JUST THE MORE THOUGHTFUL GUYSâ JOB? "I think every driver is an ambassador of the sport without a doubt, just some have a louder voice. Winning races, running up front, those types of things give you a louder voice. Each individual has a responsibility to pick how they want to carry it from there. I am privileged, very privileged and it is something not lost upon me that with winning a championship sometimes that voice gets louder and heard in different areas than perhaps I would even think it would have been. That is a tremendous opportunity to help out. I am glad to do that."
LOOKING AHEAD A WEEK, WITH THE FORMAT OF WINNING TO GET IN, THIS TALLADEGA RACE COULD BE ONE THAT AN UNDERDOG GOES FOR AND WINS. DO YOU VIEW TALLADEGA AS A LOTTERY BALL, ANYONE CAN WIN TYPE OF RACE? "I donât know if I really see any favorites. It is hard because this new car has really changed the game in a lot of different ways. I think it made plate racing better. I think this year it has been better racing than last year. All we have is Daytona to sample from but we had the Unlimited, Duels and the 500. I felt like the racing got better in the sense that last year at Talladega we saw it get single-file against the wall and nobody could make a move and we saw that a little at Daytona as well. That seems to not be the case with this package. I am not 100% sure of that though. It is hard to give you a great answer for that. Talladega will always be a wild card in some sense because of how big and wide it is and the lack of handling. It has always been that way for some reason. Obviously I got my first win there in kind of a wild card fashion. At the end of the day, I feel like the person that wins there deserves it and made a great move. I donât think of it as a lottery. I never feel that way about it. I always feel like the person that wins there made a great move to make it happen and in that sense there are 25 or 30 drivers that are very skilled and talented enough to pull that off. It is still a bit of a wild card in that sense because it isnât what we are used to. We are used to it being maybe 15 or 20 drivers. Do you add another pool of five or 10 drivers and teams into the mix that are capable of winning, yeah, and I think that is great. With the new format, that can change everything, assuming that team can stay inside the top-30 because I donât personally expect there to be more than 16 winners. That could be very significant."
BRAD KESELOWSKI CONTINUED ⦠WITH YOUR BLOG, SOME OF THE FANS RESPONSE IS THAT YOUR BLOG HAS BEEN INSPIRATIONAL TO THEM. WAS THAT YOUR GOAL? WHAT KIND OF BENEFIT DO YOU GET FROM IT? IS IT LIKE A THERAPY? "I think you can look in this room and most the media would tell you that the sports world is evolving, at least in a media sense, and so it is a challenge to me to be able to put out great content for not just my fans but all of our sports fans. I understand some of the challenges that are within this room to get those messages out and it seems like specifics of this sport, those messages always seem to get stuck in some kind of PR front or branding front and that was always very frustrating to me personally. That was kind of a way for me to escape that and get out great content and also at the same time it is personally rewarding as a way to engage some people that I wouldnât have been able to really reach before. I think it has had a further reach than I thought it would, which is great. Certainly in some ways it serves as therapy and way for me to talk and take a more measured step to not get in trouble like I used to all the time. That is good for me. It is certainly good for Roger (Penske) and I am having fun with it and am really thrilled there has been a response at all. I am happy and humbled by that."
"I have a list of ideas, so I will be good for the next three or four weeks. It is hard to pick which one I want to use at any time and it seems like as I come out with different ones about three or four more hit me. I feed off of this group and hopefully this group feeds off of some of the work I do. I really think it is great for everybody. I am just having fun with it and rolling with it."
NEXT WEEK IS THE 20TH ANNIVERSARY OF (AYRTON) SENNAâS DEATH. DO YOU REMEMBER MUCH OF ANYTHING FROM THAT? "Yeah, for me personally it reminded me a lot of when Dale Earnhardt died in the sense of the kind of general mood and atmosphere within my own familyâs household. My dad and brother used to wake up early and watch the Formula One races and I was only nine or 10 years old but I can remember my dad was a big Senna fan and I can remember that he was never really a loud cheerleader type but I can remember him being more so of that than anything else I have ever seen which was always unique to me. I just remember the somber tone in the household. My dad raced and so that brought up a lot of questions as a kid that I would ask my mom and dad. There was a sequence of untimely accidents at that time. I can remember that one and the death of this one driver - my dad raced in the ARCA series at the time and there was one driver I was a huge fan of outside of my dad and he had died I want to say a year earlier. I think he died in 93. His name was Chris Gehrke and I remember when he died my parents wouldnât tell me. We were watching TV one night and one of those memorial pictures came up. I remember that and my parents rushing me off to go take a bath but I had already seen it. It was too late. That brought up a lot of questions when I was a kid and it was really hard for them to explain to me when I was eight, nine, 10 years old. Those are the memories I have."
BRAD KESELOWSKI CONTINUED ⦠DO YOU FEEL YOU WOULD HAVE WON A CHAMPIONSHIP WITH HENDRICK AS QUICKLY AS YOU DID WITH PENSKE? "I donât know. I feel lucky to be where I am at. I feel lucky to be with Roger and very fortunate. It is a really tough question to answer because it is a fork in the road moment and obviously I didnât go down that direction. I am not going to say I havenât thought about it. Absolutely I have thought about it but part of me says I would have never met Paul (Wolfe) and would have never gotten the chance to work with him if it wasnât for that. I definitely think I could have made a large contribution to where they are at but then again, somebody else would look across and say they have won two out of the four championships since that happened so what more could you have made for a contribution? I am not sure but I definitely feel like I could have helped out."
PaddockTalk Perspective
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Kosher Pickle Factory Info
The Kosher Pickle Factory is made possible by a grant from ok kosher certification where they do kosher without compromise.
- - - - - - - - - - - - - - - - - - - - - - - - - - -
How to get the Kosher Pickle Factory to come to your school, shul, venue or private party or home event?
Cost: $750 (Plus $3.00 per person and travel costs based on location.)
Running Time: 1 hour (the show can be shortened)
What you need to do? Nothing, just advertise it.
Do you have any PR? Yes, we have a press release for your papers, and we have a ready made postcard.
Suitable Ages: 12 and up. The program is geared for teens and adults, we do have a shorter version for younger ages)
What you should charge? We suggest charging at least $18 per person.
What people say about it? "Funny, Educational, and Tastes Great!" ...."Best Adult Education class ever!"
What will KPF bring? The Pickle Rabbi (Rabbi Mendy Margolin or Rabbi Shmuel marcus) bring jars, pickles, reading cards, the garlic, dill, spices, and the entire show with props.
What will your people leave with? 1 jar of kosher pickles valued at $6.00 and a much better understanding of Kosher and pickles, and a night to remember!
RSVP your show today! (by making a $125 deposit at www.jewishcypress.com/donate
E-mail the Pickle Department Here!
Read More on Pickles here!
Past Shows:
Redondo Beach, CA, Pacific Palisades, CA, Florida Tour, Newport Beach, CA, Los Alamitos, CA, Cypress, CA, Mission Veijo, CA, Mill Valley, CA, University of SC, Laguna Nigel, CA, Los Angeles, CA, Founten Valley, CA, Seal Beach CA, Atlanta, GA, Coronado, CA, Los Angeles, CA, Peabody, MA, Penn State University, Tri Valley, CA
Newsday Lubavitch.com

subset: commoncrawl


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VOLUNTEER organisers of a popular, free annual outdoor art exhibition, held below the Monash Freeway at Richmond, say it could fold for lack of funding.
Co-ordinator Robert Lee says the non-profit Art at Burnley Harbour's quirky location – in an industrial underpass, yet beside the Yarra River and wetlands – attracts people who don't frequent art galleries.
He says there are families on a day out, cyclists from the bike path and tourists from cruise boats.
But collectors and interior decorators have also started coming.
Last year a woman from Toorak, across the river, bought eight paintings, picking them up in her boat.
She said they were Christmas presents for her grandchildren as young as one year old, because one was never too young to start an art collection.
But Art at Burnley Harbour, on this year for three days, from Friday, could be the victim of its own success.
It started in 2004 with 120 artworks over two days, and now has more than 400 pieces from 109 artists over three days.
Applications for grants and corporate sponsorships had failed. The event costs just $6500 to stage but insurance costs are rising.
The Contemporary Art Society of Victoria – of which Mr Lee is president – had noted artists Albert Tucker, Sidney Nolan and Arthur Boyd as members when it started in 1938. But ''they didn't leave CAS any money'' and it is not for profit, he says.
At Art at Burnley Harbour, artists pay a $7 entry fee and the Society gets 25 per cent commission on each sale – works range in price from $70 to $2000 – but that is ploughed back into security, insurance, cleaning and portaloos.
"Everyone congratulates us, we get lots of really good feedback but I point out that it's all put on by volunteers," Mr Lee says.
"It's really hard to raise funds, put on an event of this size, promote it and keep costs down. We don't know whether we're going to be able to afford to do it again. If a benefactor is out there who could love to assist, we'd love it."
The event has grown from word of mouth and has added a pop-up cafe, children's drawing area and free dog food for canine visitors.
This year 35 sculptures for sale will be placed among the wetlands.
Mr Lee says Art at Burnley Harbour is a simple but effective way for artists and the public to interact outside the "white cube environment" of a gallery.
"I get great satisfaction when families bring their kids in a relaxed atmosphere to see art and the parents talk to the kids about the art, and the kids ask questions.
"It's a wonderful, easy way to enrich children's lives, and it's free."


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420 East 51st StreetApt. 5H
Between First Avenue and Beekman Place Beekman
This home has been sold
We have 6 similar homes for sale.
Subway Lines
Nearby Subway Stations
- Price$378,000
- TypeCo-op
- Bedroom1
- Bathroom1
- Rooms3.5
- Exposure North
Key Features
- Doorman
- Elevator
- Pet friendly
- Full city view
- Central air
- Dining room
- Dishwasher
- Great closet space
With quite northern townhouse views, overlooking the tree lined East 51st Street; this spacious one bedroom apartment offers a generously proportioned, highly functional layout with separate dining area, renovated windowed kitchen; including granite counter tops, dishwasher, and GE appliances, hardwood floors, newly renovated bathroom, and tremendous closet space throughout providing for maximum storage.... 420 East 51st Street is a well established full service coop, providing 24 hour doorman, live in super, planted roof deck with exceptional city and East River View, discounted garage for shareholders, updated laundry room, bike room and storage. Washer /dryers are allowed with board approval. Pied a terres and subletting are permitted.
Agents Ask us a question
firstname.lastname@example.com Email Me See my 3 other sale listings
firstname.lastname@example.com Email Me See my 1 other sale listing
Living in Beekman
This neighborhood where Irving Berlin once resided, is composed of beautiful townhouses and grand co-ops situated upon just a few square blocks. Because of its size, the number of Beekman Place apartments for sale at any one time, whether co-op or condo, is limited — but the Beekman area is worth the wait! The neighborhood centers on Beekman Place, which is two blocks long, lined with grand buildings such as One Beekman Place, a co-op with a garage and an Olympic pool. In fiction, that East Side co-op was home to the madcap Auntie Mame; in fact, it contains what many believe to be New York City's grandest and best apartments.
420 East 51st Street
3rd Ave - 53rd St7 mins
3rd Ave - 53rd St7 mins
3rd Ave - 53rd St7 mins
This is a full-service co-op located on a lovely tree-lined street that turns into a cul-de-sac. The bulding features oversized new windows, a renovated roof deck with planting garden, and panoramic views. Staff includes a 24-hour doorman, a live-in super, and private neighborhood security. The building is pet-friendly. Pied-a-terres are permitted, as are guarantors and co-purchasing on a case-by-case basis. The on-site garage is discounted to shareholders. Basic cable televison, water and gas are included in maintenance. Private storage, central laundry room and bike storage are available.
Additional features of this building include: Neighborhood Private Security, Renovated roof deck with planting garden, On-site garage, Basic cable included in maintenance, Bike storage.
- Postwar
- Built in 1962
- 13 floors
- 110 units
- Elevator
- Pet friendly
- Doorman
- Central laundry room
- Common roof deck
- Common terrace
- Garage


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The method of to-day that came into its own during the war is known as the "cold-pack" method of canning.
(a) How and when should jars be closed in the cold-pack method?
It is used rather extensively in the South, where they say the conditions do not favor "cold-pack."
For this "cold-pack" method you can use whatever equipment you have in the kitchen.
They are the "open-kettle," the "intermittent," the "cold-water" and the "cold-pack" methods.
Blanching is what makes for success in the cold-pack method of canning.
(a) How should foods be packed in jars in the cold-pack canning method?
Mix the two foods in any desirable proportion and, for the cold-pack canning method, put the food into the jars.
Such a combination may be canned safely by either the open-kettle or the cold-pack method.
"cold-pack" simply means that the products are packed cold in their fresh and natural state in the glass jars or containers.
cold pack n.
A compress of gauze, cloth, or plastic filled or moistened with a cold fluid and applied externally to swollen or injured body parts to relieve pain and swelling.


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|Bollywood Dancing for Kids
Event Type: Children's Learning and Leisure
Age Group(s): 7-10
Date: No Dates Found!
Start Time: 3:00 PM
End Time: 3:30 PM
Back by popular demand! Join teen instructor Meghana Jampana to have fun learning Bollywood Dancing during this three week course. Ages 7-10 years. Attendance at all classes is mandatory to participate. Registration is required.
Location: Craft Room
Contact: Info Desk
Contact Number: 856-234-7319 x 333
Presenter: Meghana
Status: We're sorry. This event is currently full.
|Please Note


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JessNEEDSaJobModel Female Puyallup, Washington, US
My MM URL: http://www.modelmayhem.com/jezzmarie
Mayhem # 783401
About Me
i want to work.
im only 5'1" && 100lbs.
&& i know that in modeling you should be at leat 5'5" if not taller but im really intreted in doing print work not so much runway.
but dont get me wrong i wouldnt mind doing runway eather.
im a very outgoing person but can be a lil shy.
this is all new to me so i dont really know how im ganna be infront of a camra but im pritty sure ill like it!:]
im into fun kid like stuff
toys ,dolls ,cartoons ,ext.
im also in to GLAM.edge type stuff as well
but i like to be very girly.
bra & paties (if your lucky)
i dont mind dark stuff with blood && guts that could be fun?:]
and dont mide getting a lil silly sumtimes eather.
&& i have no pro. pics. i need to get sum done but with no job right now or car its hard to do anything. lol :]
if your will to shoot me please let me know thanks
Have you worked with JessNEEDSaJob? Add Credits for JessNEEDSaJob! >>
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Endothelial Cell Proliferation in Serum-Free Medium
Award Information
Small Business Information
Quality Biological, Inc.
7581 Lindbergh Drive, Gaithersburg, MD, 20879
Principal Investigator
Name: Ronald L. Brown
Phone: (301) 840-9331
Phone: (301) 840-9331
Business Contact
Phone: () -
Research Institution
AbstractA precisely defined serum-free medium would be of inestimable importance for gaining specific information concerning phenom such as the proliferation of endothelial cells and the role these cells play in the process such as cardiovascular disease, wound healing and responsiveness to growth factors and platelets. We will develop a serum- free medium that will support the in-vitro proliferation of human endothelial cells and their role in Cardiology. Serum-free culture of endothelial cells requires the presence of ill-defined components as conditional medium or a complex extracellular matrix. The initial subculture of endothelial cells has also required the presence of serum for at least one to two passages. The research herein will develop serum-free medium and the technology to avoid the use of serum or any other ill-defined component. We have developed serum-free medium that in combination with recombinant acidic or basic FGF supports the proliferation of porcine abdominal aortic and cerebral microvessel endothelial cells. We will develop serum-free medium for human umbilical cord endothelial cells that does not require the present of serum for the initial passages, conditioned medium, or an ill-defined extracellular matrix.
* information listed above is at the time of submission.


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- VIC - Melbourne
Happy Glaze
Happy glaze is a paint-your-own ceramic cafe.
Happy Glaze stock a variety of ceramic bisque items including:
- Plates
- Mugs
- Household items
- Decorative figurines
Painting ceramics appeals to both adults and children alike,express your own creativity with no experience required...
Too young to paint? Why not capture your baby's hand or footprint onto pottery for you to keep forever? We are here to help you complete a beautiful keepsake piece.
Come along on your own, with a group of friends.
After something a little different for a birthday party?
Kids of all ages love painting. Call to book your child's party at Happy Glaze.
No mess, no fuss.
They supply a piece of pottery to paint, unlimited cordial and a selection of party food.
The birthday child receives a commemorative plate.
All you supply is the birthday cake and the rest is done.
Call in to see the range and discuss prices.
- Time:
- Please see website for opening hours.
- Location:
- Happy Glaze, 52 Forest Rd, Ferntree Gully, Melbourne, VIC Map it
- Ages:
- Baby+Toddler, Preschool (1-4), School Age (5-9), Teenage (10+), All Ages / Family
- Cost:
- Prices differ.
- Event Info:
- Visit Website
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Greensborough War Memorial Park Greensborough


text:
Robert McNeillSpring Planting, from the project The Negro in Virginia
gelatin silver print
9 3/8 x 7 1/4 in.
Smithsonian American Art Museum, Museum purchase
The position of the plowman precariously balanced on his rig with no distance between him and the viewer conveys the tenuous position of black farmers and sharecroppers in the South during the 1930s. McNeill's dignified image of a humble laborer was among a series of photographs he created for the 1940 publication, The Negro in Virginia, sponsored by the Works Progress Administration.


text:
Dismiss Notice
Welcome To CK5!
Registering is free and easy! Hope to see you on the forums soon.
Score a FREE Membership sticker when you sign up for a Premium Membership and choose the recurring plan.
finally saw fast and furious
Discussion in 'Audio' started by Prembird, Jul 8, 2001.


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Seven Ways to Eat Prickly Pear Fruit
Wayne Scalaro's (of Rokerij) Prickly Pear Margarita.
Zach Fowle
Lately, we've been a little obsessed with this whole state food question. We've asked readers (who've already narrowed it down) to tell us which they'd choose: Sonoran hot dogs or prickly pear fruit.
We're not going to lie, we are pulling for the prickly pear. By way of convincing you, we now present eight ways that you can consume what might seem a hard-to-eat fruit.
7. Cahill's Prickly Pear condiments - It would be wrong to start a post about prickly pear fruit off with any company other than Cahill, whose employees for decades have foraged their own and turn them into wonderful, sweet (jellies, jams and marmalades) and savory (BBQ sauce and mustard) condiments. You can find them at farmers markets in the Valley and in online stores from amazon.com on down.
6. Ciao Bella's Prickly Pear Sorbet - Ice cream just seems cold more than refreshing when compared to a delightful sorbet. Ciao Bella makes a prickly pear flavor that you might find at your local Target, Whole Foods, Safeway or Sprouts.
5. Wayne Scalaro's Prickly Pear Margarita - The bartender at Rokerij offers up a recipe for this margarita inspired by the desert that uses a sweet prickly pear nectar.
4. Arizona High Spirits' Prickly Pear Vodka - This company makes a prickly pear vodka up in Flagstaff in the same facility as their Mogollon Brewery. The vodka, which is pink in color, lends its hand to a drink topped with agave syrup at the Westin Kierland Resort in Phoenix they call the "Native Arizonan."
3. Flancer's Prickly Pear Chicken Sandwich - This restaurant in Gilbert serves a sandwich with chicken marinated in prickly pear fruit. It's most likely the only time you want to see your chicken served pink.
2. Cheri's Prickly Pear Syrup - Move over, maple. We gladly welcome prickly pear on the breakfast table.
1. Arizona Cactus Ranch's Hangover Terminator - Many generations, as well as researchers at Tulane University in New Orleans, have hinted at the prickly pear fruit's medicinal properties that reportedly help cure hangovers and headaches. Take one teaspoon before drinking and, well, no guarantees.
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6335 N. 16th St.
Phoenix, AZ 85016
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Body & Mind
activelife by Daman
Sort by:
Time Out has a list of the best men's grooming salons in Dubai, giving you the best places to get a haircut,shave, massage, manicure, pedicure and more
Time Out Dubai has a complete guide to the best Valentine's Day dinner deals in Dubai at the best restaurants in Dubai, including the most romantic
Dubai Marina
Four hydrating therapies to see you through summer
By Amy Mathieson |
18 July 2017
A brand-new, virtual reality spinning class is taking the city by storm. Here's what you need to know
Sheikh Zayed Road
Business Bay's The Trip promises a one-of-a-kind cycling class
By Tim Skinner |
09 July 2017
50 percent discount for NBD lifestyle customers at Fairmont's night yoga
By Amy Mathieson |
04 July 2017
Fad diets, staying clear of mates and the glory of cheat days
By Will Milner |
27 June 2017
The Lost Chambers Aquarium at Atlantis The Palm is one of the locations hosting an event this week
By Amy Mathieson |
19 June 2017
Wellness events across the UAE on Saturday June 10
By Time Out Dubai staff |
06 June 2017
It's Global Wellness Day this week. Get involved with healthy activities
By Amy Mathieson |
05 June 2017


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MOUNT WASHINGTON - A lot of softball teams have good weeks during the spring. Fewer teams have exceptional weeks during any season.
The Bullitt East Lady Chargers had one of those exceptional runs last week as they blasted through four games with lopsided victories. The team also got news during the week that for the first time in fast-pitch history, the program was ranked among the top-25 teams in the state at 19th overall.
If you currently subscribe or have subscribed in the past to the The Pioneer News, then simply find your account number on your mailing label and enter it below.
Click the question mark below to see where your account ID appears on your mailing label.
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Published: March 23, 2010 at 8:06 AMI think Popeye's Bilge Rat Barges are the best ride of it's kind however it can't compete with the Mummy.
Published: March 23, 2010 at 8:27 AMPopeye's is a world class raft ride. The Mummy is a world class ride. Simply put, The Mummy is one of the best around.
Published: March 23, 2010 at 9:12 AMThis was a tough one... I haven't been on Popeye & Bluto's Bilge Rat Barges yet and I actually do like raft rides. But this one's themed so it makes it even cooler! But I had to vote for Mummy only because it's similar to the USH one (but better) and it can be ridden any time of the year. As much as I'd love to vote for Popeye, I can't really vote for it since I've never been on it.
Published: March 23, 2010 at 9:43 AMI disagree with all of the aforementioned comments. I am of the minority that believes Mummy is overrated. Good, but overrated. Some of the effects are great, but I can't get past the glow-paint and strobe lights on the coaster portion of the ride. The inanimate guards in the gold scene also ruin the illusion, and it seems that everywhere you look, you encounter an exit sign.
Popeye, on the other hand, is pure, thoughtless joy. Easily the best rapids ride around, packing in more thrills and laughs than three rides on the Mummy. And unlike most rapids rides, which seem to lose momentum very quickly, the intensity never subdues. Just when you think the ride is over, BOOM - a lift hill to what has to be the steepest rapids ride drop I've ever experienced.
This is one of the few instances where I'll vote for pure fun over atmosphere. Popeye needs to eat some spinach and smack Imhotep back to the pyramids.
Published: March 23, 2010 at 11:00 AMPopeyes is by far the best, and wettest, ride of its kind. Love the gags and the actual rapids of the ride. RotM is flat out great and a ride that should compete for the title. Both are fun but Imhotep takes this one.


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While winning four of six from the teams with baseball's second and third best records was encouraging, the true test for the Phillies came this week with a pair of road series against the first-place Marlins and second-place Braves.
Losing their first two games in Florida has put a damper on the trip and made the four remaining games much more urgent. Two series wins against their divisional foes would have put the Phils back in business. Two series losses would place them in serious trouble.
No one knows how crucial this stretch is not only for the team, but also his future, more than closer Billy Wagner. It was Wagner who gave up a game-tying ninth inning homerun to Damian Easley Tuesday night, allowing the Marlins to complete their comeback from a 3-0 deficit. It was just the second blown save of the season for Wager. The veteran left-hander is in the final year of his contract, and with several teams enduring either injuries or inefficiency from their ninth-inning specialists, Wagner could become a hot commodity if the Phillies find themselves trailing the division leaders by double digits three or four weeks from now.
"I've been around long enough to know there's a possibility (of a trade)," Wagner said. "There's no getting around that. All I can do for now is worry about getting people out."
"The way I look at it is that I'm doing the best I can for this team."
General manager Ed Wade said that he hadn't received any calls concerning Wagner when the Phillies were struggling earlier this month, but he has little doubt that there are several teams out there with interest.
"The other teams know Billy's situation," Wade said. "But I think it is preconceived that we aren't going to be interested in doing something like that (at this time). There's no question that if we got in that position, we would start getting those calls."
"If we're going to win this, we're going to do it with the guy who we wish we had as our closer all of last season."
Wagner was irritated after giving up some key hits with his slider in recent games. Cardinals shortstop David Eckstein hit his first homerun of the season on a Wagner slider, and Reggie Sanders, who was 0-for-18 with 12 K's against the southpaw in his career entering Thursday, lined a double off a Wagner slider.
"I'm done with that pitch," Wagner said. "I'm so annoyed."
Meanwhile, Jon Lieber's specialty has been an unyielding determination to not allow walks to beat him.
He won 14 games in five months with the Yankees last season by following that game plan. He won his first four starts with the Phillies this season, walking just two batters in 21 2/3 innings.
However, the bases on balls have been sprouting on his line score of late, and the wins have become fewer and farther between.
Lieber handed out four walks to the Cardinals, and it wasn't a coincidence that he allowed a season-high seven runs (six earned) in an 8-4 loss to the red birds. Although the veteran right-hander avoided trouble after walking two hitters in the first inning, he was burned badly by the two free passes he handed out in the third.
After walking just 18 batters in 176 2/3 innings last season, Lieber had walked 15 batters in his last five starts coming into Monday night's game against the Marlins. After going four years without walking four batters in a game, he has done so twice in the last 23 days.
It's a disturbing trend, one that is disturbing Lieber enough to send him zipping to his car before reporters could ask him about the loss of control.
That left it up to pitching coach Rich Dubee to answer the questions.
"He seems out of whack right now," Dubee said. "His rhythm hasn't been there, and he's a guy who needs his command."
"You'll have to ask him (if he's upset). I'm sure he's not happy about it, that's for sure."
The good news is that he didn't issue a walk through six innings of work Monday night, but did give up six hits and four earned runs, suffering the loss and falling to 5-4 on the season.
Randy Wolf will start Wednesday and look to salvage a game for the Phillies against the Marlins.
Wolf isn't showing the occasionally dominating stuff that he had during the 2002 and 2003 seasons, but he has gotten into a pretty consistent groove in May that is giving the Phillies an opportunity to win his starts. He has gone six innings in two starts this month, seven innings in the other two. He has given up two runs in three of the starts, three runs in the other.
"I'd rather be the guy who's consistently good than great once in a while," said Wolf, who gave up two runs (one earned) in six innings Friday. "The biggest compliment I can get is for my teammates to come to the ballpark and think, 'We have a good shot of winning today. Wolfie's pitching and he'll probably give up two runs, so all we need is three to win the game.'"


text: Hope you are Doing well...!!! I have urgent requirement for below position. Please go through the job description and if you are interested kindly let me know. Please revert me on swam...@sysmind.com *Role*: Mobile Test Analyst *Location: *Minneapolis,MN *Experience*: 6+ Years *JD:* a) Resource should have worked for at least 4 years in Stores with hands on testing on Backroom, Inventory management, Price management, Order Management (Sterling OMS is common across) and RMS, using TC70 b) Knowledge on End to End flow is very critical c) Fundamental and thorough knowledge on device testing d) Apart from functional – there is device testing skills that will require deep knowledge of device – a. for e.g. Device Installation testing, Connectivity testing, Firmware testing (third party firmware), OS Test, Wrapper Test, Touch screen test, Scan test, Performance test (device level), Voice & Video e) Note: if the resource has been working only on wrapper projects in Store, (s)he will have good knowledge on device levels mentioned above but may not have functional knowledge f) TC70 is predominantly used in Store level so in my opinion in-depth store knowledge combined with device knowledge is the key ask g) Resource should be able to differentiate between functional issue and device related issue and technical issue, should be capable of getting to logs and pulling errors out of the device h) Understanding of basic working technicalities with the device i) POS knowledge with Store Knowledge and Mobility knowledge j) Person who has worked with a leading Retailer in Stores with hands-on device testing skills for a minimum 4 years should be fine. k) Should have proficiency in SQL scripting to generate data sets for quick testing, can thus attempt to reduce the dependency on others -- -- *Thanks & Regards* *Swamil Singh* | SYSMIND, LLC [image: https://newoldstamp.com/editor/profilePictures/profile-b15c8fc3ea4630e2ca604f11e3e951c7-41898.png] Phone: 609-897-9670 x 5121 Email: swam...@sysmind.com Hangout: swamil.sysm...@gmail.com Website: www.sysmind.com Address: 38 Washington Road, Princeton Junction, NJ 08550 -- You received this message because you are subscribed to the Google Groups "SAP-SAP" group. To unsubscribe from this group and stop receiving emails from it, send an email to sap-sap+unsubscr...@googlegroups.com. To post to this group, send email to firstname.lastname@example.com. Visit this group at https://groups.google.com/group/sap-sap. For more options, visit https://groups.google.com/d/optout.


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Katy Perry takes trip to Madagascar for UNICEF
Listen to Katy Perry on iHeartRadio
Katy Perry is speaking about the recent trip she took to Madagascar as her first visit to the country on behalf of UNICEF.
In a statement, she says that her "eyes were opened wide" by witnessing the lack of basic health and education necessities available to the people of the African island, which is one of the poorest countries in the world.
Katy visited several parts of Madagascar, "from crowded city slums to the most remote villages," participating in activities with children. The pop star also went to a nutrition center, where she learned about the high rates of malnutrition in the country.
Meanwhile, Perry has been added to the line-up for the upcoming ASCAP "I Create Music" Expo. Katy, who is coming off her recent split with John Mayer, is joining artists like Ne-Yo, Lionel Richie, Diplo and Big Sean for the ASCAP Expo.
View more videos at: http://nbcnewyork.com.


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Section blanking
From Uncyclopedia, the content-free encyclopedia
Section blanking describes cases where someone deletes all the content from one or more sections of an article. This "someone" is often "anonymous or an unexperienced user." When registered Uncyclopedians delete all the content from a section, it is proof that the original material was not very clever. When Admins delete all the content from a section, the technical term is "preparing the article for featuring."
Two thousand years ago, section-blankers were part of a commonwealth that ravaged Ancient Rome. Section blanking was made a crime, with guilty parties sent to prison for two years.
In the vicinity of Flint, Michigan, the terminal stages of "urban renewal" are using section blanking. Entire sections (one-mile-square subdivisions of townships) are being visited by bulldozers, which remove the tenements and slums built under previous "urban renewal" programs, after politely requesting residents to please find a different ghetto.
edit Section filling
Section filling is the contrary of section blanking, but the effects of which are as devastating. This technique, previously often used by romantic writers, has become a cliché.
edit Blank sections and blanked sections
Blanked sections should not be confused with sections intentionally created blank. Blanked sections tell nothing, while blank sections can tell a lot. An example is below:


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The AFL tried to stop triple premiership player Jason Akermanis from bringing his football roadshow to Tuggeranong, the controversial footballer said yesterday.
Akermanis will play up to four games for the struggling NEAFL club, with his first against the GWS Giants reserves at Greenway Oval on June 23.
After the match he'll speak during a function at Tuggeranong's Southern Cross Club before returning to Melbourne to race superkarts the following day.
Tuggeranong is set to lose $44,000 in annual funding from the AFL Canberra development fund, which ends next year, and is hoping Akermanis's presence will help the club cover most of that loss.
Since retiring, the former Western Bulldogs and Brisbane Lions midfielder has been cashing in on the lucrative speaking tour, as well as playing one-off games for country football clubs around Australia. He said it was a win-win situation, with struggling clubs able to make much-needed revenue.
But Akermanis said the AFL, which controls the NEAFL, wasn't impressed he was pulling on the brown and gold.
The Canberra Times believes the AFL was concerned Akermanis playing in the NEAFL belittled the league, which it hopes to become as good as second-tier competitions like the VFL and the SANFL.
''I was always happy to come and the club wanted me to come and the AFL, who run that league, didn't want that to happen because I think it's against one of the AFL's teams but that's their problem,'' Akermanis said.
''We did everything by the rules and there's nothing stopping us playing ... This is [Tuggeranong's] way of finding other ways to create revenue and the AFL, for the life of me, want to take the money yet they want to stop them from making money, which made no sense.''
NEAFL eastern conference general manager Jack Masters said it was within the rules and the governing body had no problem with what the Hawks were doing.
Akermanis drew parallels between his old club and himself – having to travel to make money.
The Bulldogs used to sell one home game to Canberra, and now play matches in Darwin.
''The Bulldogs had to travel and make money and that's what they do, a bit like what I do for clubs. They get $400,000 to play in Canberra and Darwin, financially that was a decision that they had to make ... you gotta do what you gotta do,'' Akermanis said.


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Occupational Therapy/Crafts
Occupational Therapy
In Occupational Therapy, each student participates in an interview and evaluation by one or both of our registered occupational therapists. Students discuss their goals and interests for developing increased independent living skills, education, training, and employment. Evaluations include fine and gross motor skills, muscle strength and endurance, balance and coordination, tactile sensitivity, range of motion, reading and math comprehension, and other areas that affect a person's ability to live independently or to find employment. Therapeutic activities, exercises, adapted techniques, strategies for maintaining family and social interaction and employment productivity are recommended and taught as necessary. Our students find the evaluations, recommendations, and training provided by our experienced OT's to be remarkably enabling, making Occupational Therapy truly invaluable.
The Crafts class is always one of our most popular areas. As in the Industrial Arts class, students in the Crafts class learn and re-learn many leisure time activities and handcrafts. Students who have discontinued previously enjoyable hobbies and crafts due to loss of vision learn tactual techniques and methods of adapting their skills. Students have many opportunities to learn unique leisure activities and satisfying crafts. From leather stitching projects to pottery to complex needlework, students learn that there are a wide variety of techniques available that enable them to enjoy both new and old activities. Some people are looking for pleasant pastimes that they can enjoy with friends, such as leather craft or mosaics. Other students are interested in skills like sewing or knitting that help them maintain their homes and families. Often students become excited when they learn that hobbies they once thought they could no longer practice, such as crocheting or macrame, are still quite possible when a different approach is taken. Whatever their interests, our students always find our talented OT's and occasional guest instructors remarkably creative and ingenious. The only complaint we ever seem to get about our Crafts classes is that there just aren't enough of them.


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Sierra Nevada College graduates will be educated to be scholars of and contributors to a sustainable world. Sierra Nevada College combines the liberal arts and professional preparedness through an interdisciplinary curriculum that emphasizes entrepreneurial thinking and environmental, social, economic and educational sustainability.
We make the Admissions process seamless and personable. Each of our staff is dedicated to helping you fully understand whether our liberal arts college is a good fit for your educational career.
learning is not a spectator sport. We're a college of active learners and educators.
We engage, we collaborate, we debate, we question, we critique, and oh, do we grow. That's what all this is about: inspirating poised, life-ready, critical thinkers.
Nowhere on this planet can you have a college experience that comes anywhere close to this.
I give Sierra Nevada College (SNC) and its fiscal and/or media agents permission to use my name, likeness, image, voice, and/or appearance as such may be embodied in any pictures, photos, digital images, video recordings, audiotapes, live audio and video streams, and the like, taken or made on behalf of SNC, its programs, or affiliated activities. I agree that SNC has complete ownership of such content, including the entire copyright, and may use it for any purpose consistent with the SNC mission.
These uses include, but are not limited to illustrations, bulletins, exhibitions, videos, prints, reproductions, publications, advertisements, and any promotional or educational materials in any medium now known or later developed, including the Internet. I acknowledge that I will not receive any compensation and hereby release SNC, its agents, and assigns from any and all claims which arise out of or are in any way connected with such use.


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Amigurumi Drawstring Purses Pattern Set by Amy Gaines
Welcome! You are browsing as a guestWould you like to see 7 projects made from this pattern and much more? create a free account What am I missing?
© AmyGaines
© AmyGaines
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© Shady
Amigurumi Drawstring Purses Pattern Set
This pattern is available for $5.00 USD
buy it now or visit pattern website
buy it now or visit pattern website
This pattern set will instruct you on how to make the following:
Ice Cream Cone Purse
Starwberry Purse
Mushroom Purse
The pattern is easy to follow and includes lots of photos to help you along.
4.25 mm crochet hook
worsted weight yarn
green felt
yellow small beads
Large beads- red, white, pink ( for the end of the drawstrings)
For more information, see: http://www.etsy.com/shop/AmyGaines


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Score a promotional touchdown with this small inflated promotional football.
A unique and fun shaped custom football drawstring backpack that is perfect for any football fan, school or summer camp!
If you're looking for a great promotional item to commemorate the big game, then this full size custom football is the ideal choice.
This foam custom football will be a big hit with recipients and your brand!
Perfect for team events! The football shaped Fidget Spinner Custom Stress Reliever is designed to relieve stress and anxiety. A perfect way to relax before the big game!
Beat the heat this summer with a HydroPouch! collapsible custom water bottle with spray top.
Support your favorite team with these great cotton velour football shape custom towels imprinted with your company logo.
This football shaped promo journal makes a great promotional item or gift for that favorite team or a sports related event.
This football shaped promotional stadium cushion is great for sports fans.
Use football promotional novelties for your next promotional campaign! No matter what your cause, use football-shaped items to send the right message. With the wide selection of football-related promotional products ePromos provides you, your company logo will look great imprinted on these promotional sports products!


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This comprehensive method for classroom or individual study comes in two sections. The first section teaches chords and accompaniment, while the second teaches note reading. This flexible method
Offering the ultimate in sound educational value and maintenance of student interest, this course of instruction for class guitar is truly revolutionary! Each book has more than 45 songs and is 96 pages
This item has been discontinued and we can only sell our remaining stock. Add to cart to check availability and for more information.
Here is a method that uses real songs to teach you all the basics of acoustic guitar in the style of The Beatles, Eric Clapton, John Mellencamp, Indigo Girls, Robert Johnson, James Taylor and many others.
Orchestrate Your Inbox
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Pepper Music eClubs
Everything you need for the upcoming season and special recommendations from our editors! Each club is sent monthly (unless otherwise noted).
General Music
Music Library
Cued In - The Pepper Blog
A weekly digest of insightful articles, helpful resources, and interviews with leading composers and arrangers
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Howrah Vacation Packages
Howrah is near many of Kolkata's more popular attractions. Two destinations that should be on every visitor's to-do list is the fascinating Botanical Gardens and the ever-popular Alipore Zoo. And if your schedule allows, be sure to visit National Library and Belur Math.
Holiday makers in Howrah, interested in finding out what makes the area tick, should head to the nearby cultural attractions. Explore, learn and be amazed in the most popular museums which include the Academy of Fine Arts, Indian Museum and Asiatic Society.
If the sun is shining, follow the locals and appreciate the nearby green spaces. If you want to rest a while in a little nature explore Milennium Park.
While you are here, head to some of the local sights such as the St. Paul's Cathedral, Maidan and Kalighat. These are a magnet for travellers from all the corners of the globe. Learn about history by visiting Marble Palace.
Find the perfect accessory, stock up on groceries or just people-watch at nearby fashion hotspots such as New Market and the Forum Shopping Mall.
Those with a love of cricket will love the energy at iconic Eden Gardens, home of the Bengal cricket team and the much-loved Kolkata Knight Riders. Relive the match's greatest moments with local fans after the final whistle blows in one of the nearby pubs, clubs or bars.
After a long day discovering the sights, sounds and smells of Howrah, relax for the night in one of the area's popular bed-and-breakfasts, hotels or short-stay apartments. The Oberoi Grand, Kolkata, the Peerless Inn - Kolkata and the Taj Bengal are just three of the best places to stay while you are enjoying this special area.


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Pre-Report: Energy Efficiency Workshop I: Operational & Facilities Strategies
Energy Efficiency Workshop I - Operational & Facilities Strategies
The Pew Center on Global Climate Change hosted its first Corporate Energy Efficiency Workshop on July 16, 2008, and focused on Operational and Facilities Strategies. Presentations from the workshop are available below. Click on the speaker's name to view the presentations.
Welcome & Opening Remarks
Eileen Claussen, President, Pew Center on Global Climate Change
Jo Cooper, Vice President, Government and Industry Relations, Toyota
Lunch Keynote
R. Neal Elliot, PhD., Industrial Program Director, American Council for an Energy-Efficient Economy
Company Panel 1
Lisa Shpritz, Senior VP of Corporate Workplace, Bank of America
Bruce Schlein, Vice President, Environmental Affairs, Citi
Company Panel 2
Vanessa Stiffler-Claus, Manager of Federal Affairs, Deere & Co.
Deborah Kuo, Director of Real Estate, Exelon Corp.
Company Panel 3
Paul Vitello, Director of Environmental Sustainability, United Technologies
Wendy Graham, Clean Energy Market Manager, Air Products
|Workshop I:
Operational & Facilities Strategies
|Workshop II:
Supply Chain Strategies
|Workshop III:
Products and Services
|Workshop IV:
Sharing Best Practices
In partnership with:


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Formulated in the label's base of Aoyama, Tokyo, the retaW Allen Fragrance Body Shampoo combines elements of lily and an understated mix of lemongrass, grapefruit, mandarin and lavender. The formula was designed for all types of skin to leave a soothing and refreshing scent after usage.The retaW Allen Fragrance Body Shampoo effectively furthers the brand's dedication to creating new and innovative ways to maintain the body and skin through organic materials and natural plants and essentials oils.
For more information about returns please click here.


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Looking Back through the Office Window
Yesterday, my husband left for work early in the morning. It wasn't entirely unusual, except I dressed our 6 week old twins in "special clothes" after I nursed them, and we saw Daddy off. I cried, overwhelmed at what I would do after having had his untiring help at home for an extended period of time. "I can do this," I thought, "Surely, I can do this." And then I squalled some more.
But that wasn't really yesterday. It just seems like it, and tomorrow I return to my career after a 5 year hiatus. But when I think about them laying on that blanket in the den and me crying, it does seem like only moments ago…and I just cannot believe that time has somehow moved at warp speed.
[pullquote type="2″ align="right"]"I used to hate that ubiquitous advice: "Enjoy it! They grow so fast." "[/pullquote]
I've had an extreme amount of guilt. I am an uber-perfectionist, OCD, and have a Cancer Zodiac sign, so I want a measurable amount of success on my time home with my kids. Somehow, as I have tried to mentally prepare myself for tomorrow and the weeks ahead, I have felt that I missed something, or failed to do something.
And then I think about all of the moments I have had with these children, grocery trips, hiking, shopping, playing, crafting, crying….laughing. I think about times when they were sick and I would spend the days making them comfortable or accommodating whatever they might have needed. I beat myself up a bit for the days when I was preoccupied with housework or volunteer work or other "pressing" things, but even on those days I was just a "MOMMMMMMMYYYYYY!" away, and, on those days they learned some independence. I've managed to prepare them scholastically for Kindergarten (although we've yet to see how they do socially after I peel them from beneath their smothered spots under each wing).
I am going to try to always remember that all of these moments, including the ones right now will soon seem like a lifetime has passed us by, and, yet, will somehow seem like they just happened. I used to hate that ubiquitous advice: "Enjoy it! They grow so fast." And, now, I will shamelessly say to any mommy reading this: savor it….this moment will never, ever happen again.


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The government's to give the go-ahead to build thousands of new wind turbines off the British coast.
They'll create electricity through wind power.
Cleaner fuel
Producing energy by using wind is seen as much more environmentally-friendly than burning fossil fuels.
The new turbines will produce 5% of the UK's electricity.
That's more than 10 times what wind farms make at the moment.
A group of important engineers - The Institute of Civil Engineers - have said it's good thing but we need so much energy that wind power won't be able to provide all of it.
And some people will worry about birds and wildlife being disturbed when the building starts.


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Looks like The Landings at Steele Creek I Apartments is no longer available on Rent.com which means this information is likely out of date.
Contact for details
Fully Equipped Kitchen, Washer/Dryer Connections...Perfect Country Setting Minutes from Downtown...Playground, Laundry Care Center, Superior Service
To live well is to find a certain balance that is uniquely your own. That's what you'll find at The Landings At Steele Creek Apartments. We're the perfect blend of amenities, custom design and ideal living spaces-- all available in the perfect country-like location within your budget. Our unparalleled location puts you right where you need to be without fuss and minutes from downtown. You'll live close to all you love to experience dining, shopping, night life and sporting events. Better yet, we've designed The Landings with the simple expected comforts like vaulted ceilings and expansive closet space. Allow our superior service and hands on staff to surround you with the kind of living experience that will make you feel right at home. The Landings At Steele Creek: distinct, effortless apartment living at a comfortable price. Balance and Service as it should be.
Lease Terms: 3 to 36 Months
No Pets
120 units
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I received a copy of a letter that was sent to the "Letters to the Editor" in The Record some time ago. After reading it, I felt it an excellent letter and that the information should be shared with the good readers of the Lodi News-Sentinel. The letter covered a 30-year period regarding things that caused the burst in the housing bubble. After verifying the information in the letter, I wanted to give a brief history of why the housing bubble burst and the causes.
In 1977, Democrat Jimmy Carter signed the Community Reinvestment Act, guaranteeing home loans to low-income families.
In 1999, Democrat Bill Clinton put the CRA on steroids by pushing Fannie Mae and Freddie Mac to increase the number of sub-prime loans. In September 1999, The New York Times published an article headlined "Fannie Mae Eases Credit to Aid Mortgage Lending," which warned of the coming crisis due to lax lending policies of the Clinton administration.
In 2003, The White House called Fannie and Freddie a "systemic risk" and the Republican Bush administration pushed Congress to enact new regulations. Also in 2003, Rep. Barney Frank, D-Mass., said Fannie and Freddie are "not in a crisis," and bashed Republican-sponsored regulation legislation.
In 2005, Federal Reserve Chairman Alan Greenspan voiced warning over F&F accounting, saying, "We are placing the total financial system of the future at a substantial risk." In addition in 2005, Sen. Charles Schumer, D-N.Y., said he thinks F&F "over the years have done an incredibly good job and are an intrinsic part of making America the best-housed people in the world."
In 2006, Sen. John McCain, R-Ariz., again called for reform of the regulatory structure that governs F&F. And again Democrats blocked reform legislation.
In 2008, the housing market collapsed; Democrats blamed the Republicans.
Yup, the Republicans caused it — or did they? What do you think?
Sam West


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Monmouth - Cry Baby Bridge
Story says that a bus full of little kids ran off the bridge and they all died. Rumors are if you go there turn your lights off and put your car in neutral something will push you across to safety. After you are pushed across get out and put powder on your windows and you can see little baby hand prints.
Monmouth, Illinois
There are 411 Haunts nearby
Source: Internet
|Location Approximate!!! Care to correct it?
40.9114271, -90.6473576


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King hailed for breakthroughs in equality
ATLANTA – Against the backdrop of a looming national battle over access to the voting booth, Americans celebrated the Martin Luther King Jr. holiday Monday.
On the federal holiday marking what would have been King's 83rd birthday, people here and elsewhere celebrated King's achievements while warning that voter identification laws enacted or sought in dozens of states threaten those gains.
"You cannot celebrate Dr. King on Monday, and undermine people's ability to vote on Super Tuesday," said the Rev. Raphael Warnock, pastor of Ebenezer Baptist Church, where King preached during the 1960s.
STORY: Martin Luther King papers go online
MORE: Witnessing the dream
PHOTOS: Dedication of MLK Memorial
It was a reference to March 6, when 11 states hold primaries or caucuses for the Republican presidential nomination. Voter ID laws require voters to present some form of official identification.
During remarks at the annual commemorative King service at the downtown church here, Warnock's attacks on such laws drew a standing ovation from nearly everyone except Gov. Nathan Deal and Sen. Johnny Isakson, both Republicans, who were on the dais with him.
Civil Rights Movement
In Columbia, S.C., hundreds of people rallied outside the state Capitol to protest the state's new voter ID law, which the U.S. Justice Department has blocked, arguing it could hinder the right to vote of thousands. South Carolina plans to fight that ruling in court. Attorney General Eric Holder said he would use sections of the Voting Rights Act to ensure that the nation's election system is accessible to U.S. citizens.
Benjamin Todd Jealous, president of the NAACP, said the image of the nation's first black attorney general standing at the state Capitol "with the Confederate battle flag (which flies on Capitol grounds) waving overhead … was a very 21st-century moment."
"Here we are, 44 years since Dr. King was assassinated and we find ourselves fighting to maintain one of the biggest victories he saw in his lifetime," Jealous said. "This is the biggest attack by state legislators on access to the ballot box in more than 100 years."
Other celebrations across the country focused on volunteer efforts and more traditional ceremonies to celebrate King's life.
•President Obama and his family commemorated the day with a volunteer service project at a Washington, D.C., school.
The family greeted volunteers and helped build bookshelves in the school's library. Obama said there was no better way to celebrate King's life than to spend the day helping others.
•Washington is home to the new Martin Luther King Jr. memorial, where some braved temperatures near 30 degrees to visit the 30-foot statue of King.
Dorothy Drake, 61, and her sister, Beulah Ashby, 59, visiting from North Carolina, said they attended segregated schools and remembered not being able to eat inside certain restaurants, sitting on the back of buses and being banned from some hotels. "I woke up this morning and it gave me great joy to know that I was coming here," Ashby said. "I know it's a piece of stone, and I know it's one man, but it was just pride. Pride. Happiness."
•Vice President Biden and his wife, Jill, celebrated the holiday in Philadelphia, which has one of the nation's largest King Day celebrations.
The Bidens joined about 4,000 volunteers at Girard College to assemble and package 100,000 meals for distribution around the world by the non-profit Stop Hunger Now, said Todd Bernstein, founder and director of the Greater Philadelphia Martin Luther King Day of Service.
•In Detroit, about 2,500 people walked in honor of King. "We're just trying to keep the tradition going so history is not lost to the future generations," said DeShaun Willingham, 39, a millwright who brought his children to the walk.
•In Iowa, Gov. Terry Branstad, a Republican, told about 300 people that King's legacy is carried on by the icon's family, friends and others from the civil rights movement.
"Dr. King really led the fight against segregation, racism and injustice in this country, and he made a big difference in the lives of everyone," Branstad said.
Contributing: Matt Helms, Detroit Free Press; Jennifer Jacobs, Des Moines Register; Associated Press
Posted | Updated


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Beyond Oatmeal: Oat Recipes and Tips
10 ways to harness the health power of oats.
By Elaine Magee, MPH, RD
Ask almost anyone to name one of the healthiest breakfasts you can have, and many will say, "oatmeal." Ask why it's so healthy, and they would probably answer, "fiber." While getting more fiber is a good reason to reach for that packet of oats in the morning, there's a lot more to oats, nutritionally speaking.
Half a cup of oats will give your body a nutritional boost beyond the 4.1 grams (g) of fiber. You get some plant protein along with some smart fats (from monounsaturated and polyunsaturated fat), vitamins and minerals, and countless phytochemicals, too!
Let's do the numbers:
Rolled Oats, 1/2 cup serving (approximate values)
The health benefits of oats are thought to include limiting oxidation of LDL ("bad") cholesterol, decreasing total and LDL cholesterol, and reducing blood pressure -- all of which help lower your risk of heart disease. The fiber in oats is also thought to help you feel full longer, and reduce the amount of insulin released after meals.
10 Ways to Harness the Power of Oats
There are all sorts of way to eat oats. There's the predictable hot oatmeal breakfast (which can also be a quick snack at work if the lunchroom has a microwave). Then there are oat recipes for baked goods, like muffins, breads, and certain desserts. Crisps, for example, traditionally call for oats. Granola is a type of cold breakfast cereal that usually includes plenty of oats, too.
One of the easiest ways to enjoy oats is as instant oatmeal. Usually, instant oatmeal is quite high in sugar. But there are now some choices in the supermarket that keep the convenience but lose some of the sugar.
Here are 10 ways to eat more oats:
1. Opt for Healthier Instant Oatmeal
Most people prefer some sweetness in their oatmeal. But there's more sugar than we need in all those fun, flavored oatmeal packets, that's for sure. How do I know? I've tasted the new less-sugar types, and they taste great!
There are Apples & Cinnamon and Maple & Brown Sugar varieties, and my personal favorite, Take Heart Blueberry. A packet (34 grams) of Quaker's 50% Less Sugar Maple & Brown Sugar flavor has 4 grams of sugar (13% calories from sugar), along with 3 grams of fiber (1 gram of which is superhealthy soluble fiber). The Take Heart Blueberry variety (a larger, 45-gram packet), with added oat bran and flaxseed, has 6 grams of fiber (4 grams of which is soluble), and 9 grams of sugar (22.5% calories from sugar), plus 130 milligrams of plant omega-3 fatty acids.
2. Make Some Muffins With Oats
Look for muffin recipes that call for both oats and whole-wheat flour. This will give you a nice balance of soluble fiber (from oats) and insoluble fiber (from the whole wheat).
Oats add texture and a mild nutty taste to muffins. Use either Quick or Old Fashioned Oats, though. Instant oats (in the packets) usually add some sugar and other ingredients along with the oats. (See the Strawberry Oat Muffin recipe below.)
3. Substitute Oats for Other Fillers
Instead of adding bread or cracker crumbs to your meatloaf, meatballs, or stuffed mushrooms, add oats instead. Rolled or quick oats cook up fast and hold onto moisture well.
4. Add Toasted Oats to Other Dishes
You can add toasted oats to trail mix, or sprinkle it on top of yogurt, frozen yogurt, or fresh fruit. Or, try adding it to cookie dough in place of some of the nuts the recipe calls for. (Check out the recipe below for Cinnamon Vanilla Toasted Oats.)
5. Thicken Soups and Stews with Oats
The heartier steel-cut oats can add thickness and texture to soups and stews. Steel-cut oats are cut into bits with steel blades instead of rolled. (Rolled oats have a flakier texture and take a shorter time to cook.)
Gravitate toward broth or tomato-based soups and stews. These are typically the lowest in fat and calories.


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Your current listing is shown below:
First Baptist Church Of Lipan
P.O. Box 28
Lipan, TX 76462
Phone: (254)646-2484
Church affiliation: Southern Baptist Convention
Church confession: 2nd London Baptist Confession (1689)
Contact: Rev. David V. White, Pastor
Web page: http://email@example.com
|We are a rural reformed and reforming SBC church committed to expository preaching, the regulative principle of worship, the solas of the reformation and the proper administration of the sacraments (ordinances) of Baptism and the Lord's Supper as instituted by Christ.
If this is the listing you want to change or remove, click UPDATE below to revise or remove the entry. You will be emailed a web link at your currently registered email address. Click on that link, and you will be taken to a form for updating the information. NOTE: You must be able to receive email at the currently registered address to update your listing this way.
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Bookmark this site
October 15, 2013 marks the debut of Pearl Jam's highly anticipated 10th studio album, Lightning Bolt, on TouchTunes. Released via Monkeywrench Records/Republic Records and produced by Brendan O'Brien, Lightning Bolt is Pearl Jam's first studio album since their acclaimed record "Backspacer, "which was released in September 2009.
Since the band's 1991 inception, Pearl Jam has sold over 60 million albums worldwide over a span of nine studio albums, five live albums and one greatest hits record. In 2011, the band celebrated its 20th anniversary with a special concert at Alpine Valley, WI, and a film release, "Pearl Jam Twenty," directed by the legendary Cameron Crowe.
Timed with the release of Lightning Bolt, Pearl Jam will embark on a two-leg, 24-date North American tour kicking off in Pittsburgh on Oct. 11 and ending in the band's hometown of Seattle on December 6th.
For Pearl Jam tour dates, visit http://pearljam.com/tour.


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Agilent N3300A 1800W precision DC Electronic Load Bank for testing:
One Agilent N3306A 600W load module included; additional N3306A 600W load modules also available for hire. Up to 3 load modules can be connected to 3 independent DC supplies, or connected in parallel to a single supply, providing up to 360A of DC load at up to 60V (total 1800W). Suits testing of 12V, 24V and 48V battery banks, solar panels and power supplies.
Testing High Power Solar Cells with the N3300-series:
Application Notes:
N3306A Load Module Specifications (per module):
Modules can operate in the following modes:
Modules also have built in:
Testing multiple output dc power supplies and dc-dc converters can be very time consuming if each output must be tested sequentially. If measurements are being made through a MUX using one DMM, this is what will happen. Using the built-in measurement capabilities of the N3300A electronic loads, all outputs can be measured simultaneously. Alternatively, multiple single output power sources can be tested simultaneously.
Supplied With:
Shopping Cart Software by Ashop


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St. Mary's College
ILGU SCHOOLS CHAMPIONSHIP -- The Irish Ladies Golfing Union (Mid-leinster District) schools championship took place on Friday 23rd March 2012 at Abbeyleix Golf Club.
Over 25 schools from the district participated in the competition and over 30 clubs were represented. St. Mary's College was represented by Caroline Hewitt, Aine Kavanagh and Tara Butler. St. Mary's won the Junior Cup (this cup is for students up to Junior Cert).st. Mary's now represents the Mid-leinster District in the All-ireland Final in Milltown Golf Club on Monday 30th April 2012.The girls will compete against the winners of the Munster, Connaught, Ulster and Leinster districts. Best of luck to the girls.
2 Una CSPE class took part in a basketball challenge on Daffodil Day, 23rd March, as part of their CSPE action project fundraising event in aid of the Irish Cancer Society. Their target was to score 500 baskets in one hour but they surpassed all expectations and scored 824! The girls are still gathering sponsorship money and are very grateful for all support received in the school and community. Ms Joanne Stapleton, CSPE teacher, was delighted with the girls' display of team spirit, enthusiasm, determination and most importantly basketball skills.
Second Year student, Amy Mcdonald, will represent Arklow and Co. Wicklow at the 2012 National Art Finals in Athlone at the end of May. Congratulations to Amy and good luck in the finals.
On Wednesday 28th March, TY students, Amy Kinsella, Ciara Tyrrell, Niamh Dillon and Kathy-ann Murphy paid a visit to the People Newspapers office in Rowe St., Wexford town as part of their involvement in the TY News supplement project. The students were shown the ways in which a newspaper is produced. They were given advice on reporting, page design and copy editing. Amy, Ciara, Niamh and Kathy-ann have already submitted articles for publication and are looking forward to seeing their work in print very soon.
Bon vovage to our students and teachers who will spend some of their Easter holidays in Montfort sur Meu,as part of a French exchange programme organised by Gaelcholaiste na Mara and St. Mary's. Our students have already hosted the French students when they visited Arklow. Now, on this return visit, the students will spend time with their new friends immersing themselves in the French language and culture. The French department appreciate the hospitality and support of the families who hosted the French students and contributed to the success of the exchange.


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The 35-year-old refused to co-operate on the American Airlines flight from New York after being asked to fasten his seatbelt on approaching London.
The flight's captain alerted Heathrow police, who arrested the musician once the plane landed.
A Scotland Yard spokeswoman confirmed DMX - real name Earl Simmons - was later released with a caution.
DMX has sold more than 20 million albums worldwide and will release his sixth solo album, Year of the Dog, Again, this summer.
Launch party
He is due to perform at a secret launch party in London on Tuesday.
DMX, who has also appeared in films including Romeo Must Die and Exit Wounds, was sentenced to seven days in prison in 2005 for colliding with two cars while driving with a suspended licence.
He received a 70-day sentence for a separate incidence of speeding while holding an invalid licence in December 2004.
In June 2004, he was arrested after crashing his car into a gate at New York's JFK Airport and was handed a conditional discharge.


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A state Senate panel endorsed a series of bills Tuesday aimed at improving Louisiana's health rankings.
The Health and Welfare Committee shipped to the Senate floor:
Senate Bill 514 that would ban smoking within 200 feet of elementary and secondary school property.
• Senate Bill 513 that would create the Louisiana Obesity Prevention and Management Commission.
• Senate Concurrent Resolution 21 establishing a joint legislative committee on obesity.
• Senate Concurrent Resolution 20 directing the state health agency to seek federal approval for WIC cash value vouchers to be used at farmer's markets.
State Sen. David Heitmeier, D-New Orleans, sponsored all the legislation.
Heitmeier deferred action for a week on Senate Bill 491 that would add e-cigarette and alternative nicotine products to restrictions of the Louisiana Smokefree Air Act.


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Join Los Angeles News Group reporters in a live chat Saturday at 9 a.m. on our High School Sports page for the latest on CIF Playoffs throughout Southern California.
DOWNEY - It was their defensive abilities that brought Wilson High and Sunny Hills to the CIF Southern Section Division 2 championship game on Friday night.
But it was an early second-half goal by Claire Oates that led the third-seeded Bruins to a 1-0 victory and their second CIF title.
The Bruins' faithful cheered from the stands at Warren High and the team celebrated heartily at midfield following the final whistle. It was Wilson's first CIF title since sharing the 2008 crown with San Clemente, and its first outright title.
"It doesn't get any better than this," said Wilson coach Dalton Kaufman, championship plaque in hand. "It's been an incredible 2013."
There weren't very many creative attacks by either team - somewhat expected since Wilson (20-4-4) and Sunny Hills were defensively dominant all year, allowing just 12 goals each heading into the game.
But the difference in the game was Bruins' ability to make one of them count early in the second half.
Madi Grieve brought the ball near the top of the box before sliding it to Sierra Skelly joining the rush on the right. Skelly fired a shot on net that the keeper stopped but couldn't hold on to. Oates crashed the net and sent the rebound in to give Wilson the only goal it needed in the 44th minute.
"Luckily I was there to finish it," Oates said.
It was kind of a shock to Sunny Hills (24-2-5).
"It's rare when anyone gets behind us," Lancers coach Jeff Gordon said. "Wilson was able to capitalize."
Thirty-six minutes was a long time, like an eternity for the Bruins, to protect their net and hold, especially down the stretch when Sunny Hills threw numbers up top in an effort to produce the equalizer.
The Lancers didn't record a shot until the 54th minute, but turned it up over the final 15 minutes. Their best chance was on a 3-on-1 counterattack that came up short on an offsides call.
"That was the longest 36 minutes of my life," said defensive midfielder Katie Pratt-Thompson. "We had to keep it steady in the back. We were focused on what we wanted to do. We set a goal and that was to shut them out."
The Bruins and Lancers seemed more concerned with safe, don't-lose soccer in the first half.
There was nothing coming out of the back as the teams chose to clear balls out safely rather than try to build any type of attack, leaving both teams wanting for any real sort of scoring chances.
Wilson managed just three shots in the first half and eight for the game.
Meanwhile, Sunny Hills' attack was really non-existent until late. In the first half, the Lancers couldn't get anything going, except for a couple of long balls that Bruins keeper Samantha Moreno pulled down, against the Wilson backline of Auset Hood, Nikki Voulgaris, Amanda Empleo, Sarah Maher and Quinn Trowbridge. They finished with six shots, all in the final 26 minutes.
It's now on to the SoCal Regionals for the Bruins.
After a team picture, Kaufman told the team, "You have one more to win. Are you in or out?" The Bruins responded with a very loud, "In."


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Follow @PennGym
PHILADELPHIA – Coming off back-to-back Eastern College Athletic Conference (ECAC) championships, the University of Pennsylvania women's gymnastics team opens up the 2014 competition season at George Washington on Sunday.
For the fifth consecutive year the Quakers will open up their regular season in Washington D.C. This year's Lindsey Ferris Invitationalfeatures the Quakers,Cornell, Temple and the host George Washington. Penn finished fourth out of five teamsin the 2013 event.
-Ticket Information
-Real-Time Scoring
-Watchon George Washington's RaiseHigh.tv
2014 Season Outlook
After back-to-back ECAC championships Penn is looking to build upon the success of recent years. The Quakers will have to do so with new-look rotations in all four events, after graduating highly decorated all-around competitors Kirsten Strausbaugh, Megan Milavec and Dana Bonincontri.
"We have another exciting year ahead of us filled with opportunities," said Head Coach John Ceralde. "This season, our team is talented but young. They have the ability to take on new challenges as we continue the program's tradition and success."
Penn is looking forward to seniors Diana Moock and Nicki Valenti rising up to take advantage of those opportunities. Also taking on larger roles will be juniors Amber Woo, Wynne Levy and Kaitlyn Reszkowski.
The Quakers will welcome back junior Makeda Constable, as she returns to action in 2014, after suffering an injury two weeks prior to last year's ECAC championship.Sophomores Amber Hu and Carissa Lim, along withnewcomer Rachel Graham will also have an opportunity in a number of events for the Quakers.


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Maynor Figueroa ruled out of Cup Final
Wigan Athletic's fears were realised yesterday when Maynor Figueroa was ruled out of the FA Cup Final and the club's relegation run-in by a groin injury that will require surgery .
But Roberto Martínez, the Wigan manager, is hoping that another key defender, Antolín Alcaraz, could be available to face Manchester City at Wembley a week tomorrow. The centre half had seemed certain to miss the showpiece event with a hamstring injury.
Alcaraz will not be fit to face West Bromwich Albion at The Hawthorns tomorrow — a game that Wigan may have to win if they are to avoid relegation.
In the absence of the Paraguay player and Figueroa, who suffered his injury in the 2-2 draw against Tottenham Hotspur last Saturday, Gary Caldwell…


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Cell Biology/Models/Worms
From Wikibooks, open books for an open world
The chosen species is Caenorhabditis elegans.
Etymology: 'Caeno' and 'rhabditis' derived from Greek, means 'recent', referring to Pleistocene epoch (2.5 million to 12000 years BP), and 'rod-like' respectively. 'Elegans' is derived from 'elegant' due to the elegant sinusoidal movement of this nematode.


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by Staff Writers
London (AFP) June 15, 2014
Chinese Premier Li Keqiang begins a three-day visit to Britain on Monday, hoping to "change misperceptions" about his country and improve diplomatic relations with London.
Relations between the two countries remained frozen for more than a year due to a row over Tibet, but Li said the visit, which will include a meeting with Queen Elizabeth II, would help to "deepen co-operation in various fields".
Writing in the London Times, the number two leader of China's ruling Communist Party said his trip had three main purposes.
Along with strengthening economic ties, Li wrote that he wanted "to present the real China so as to change misperceptions and ease misgivings" and also "to draw on British perspectives and experience."
Despite the diplomatic spat, sparked by Prime Minister David Cameron's decision to hold a private meeting with the Dalai Lama in May 2012, Li called the United Kingdom "a great country and an important partner of China".
The high-ranking official wrote of his determination to modernise his nation through urbanisation and industrialisation, acknowledging that China was still "far from achieving its development goals".
He also moved to soothe fears that a more powerful China would be a destabilising geopolitical force, writing in the British paper that his country "will actively engage in global dialogue, and will promote the 21st century as an era of peace and co-operation."
Li's trip to Britain is the first by a Chinese premier since his predecessor Wen Jiabao visited in 2011. The last Chinese president to go was Hu Jintao in 2005 whose visit was dogged by protests by pro-Tibet and human rights campaigners.
China's leaders reduced diplomatic contacts after Cameron's meeting with the exiled Tibetan spiritual leader whom Beijing characterises as a "political exile engaged in anti-China separatist activities in the name of religion".
The relationship began to thaw last June, when both countries' foreign ministers spoke by phone.
That paved the way for a visit last December by Cameron to Beijing, a trip billed as Britain's biggest ever trade mission to China.
But as Sino-British relations have warmed, China's leaders have continued to react sharply to international criticism of their human rights record.
After visiting Britain, Li travels to Greece, where he is scheduled to meet with Prime Minister Antonis Samaras and President Karolos Papoulias, before wrapping up his trip on June 21.
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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
The aryl hydrocarbon receptor (AHR) regulates the toxic effects of 2,3,7,8-tetrachlorodibenzo-
Aryl hydrocarbon (AHR) is a ligand-activated transcription factor and a member of the basic-helix-loop-helix Per (Period)-ARNT (aryl hydrocarbon receptor nuclear translocator)-SIM (single-minded) (bHLH/PAS) protein superfamily. AHR mediates the toxic effects of the environmental contaminant, 2,3,7,8-tetrachlorodibenzo-
The mechanism by which AHR regulates its target genes is for the most part well understood; however, our understanding of how a transcriptionally activated AHR is regulated or inhibited is incomplete. Some of the proposed mechanisms include: (1) increased metabolism of the activating ligand through induction of drug metabolizing enzymes; (2) ligand-induced proteolytic degradation of AHR via the ubiquitin/proteasome pathway; and (3) negative auto-regulatory feedback control via the induction of AHR-dependent repressors, such as AHRR [
ADP-ribosyltransferases (ARTs), formerly known as poly(ADP-ribose) polymerases [PARPs], catalyze the transfer a single or multiple ADP-ribose units from nicotinamide adenine dinucleotide (NAD+) onto themselves or other protein substrates [
In the present study, we compared the ability of AHRR and TiPARP to repress
AHRR and TiPARP (ARTD14) are both TCDD-responsive genes [
We next confirmed the ability of overexpressed AHRR to repress CYP1A1 reporter gene activity, and determined if overexpressed AHR or ARNT prevented AHRR-mediated repression of AHR. Increasing concentrations of AHRRΔ8 repressed CYP1A1 reporter gene expression (
To determine if endogenous AHRR is recruited to AHR target genes and whether its presence at these genes would affect AHR and ARNT recruitment levels, we examined the TCDD-dependent recruitment of AHR, ARNT and AHRR to
We next determined the impact of AHRR and TiPARP knockdown on TCDD-induced CYP1A1 and CYP1B1 mRNA levels in MCF7 cells. TiPARP mRNA levels were reduced to approximately 33%–40% compared with non-targeting siRNA (NT) dimethyl sulfoxide (DMSO)-treated cells and resulted in reduced TCDD-dependent increases TiPARP mRNA levels (
We reported that TiPARP knockdown in T47D cells increased constitutive AHR expression and reduced the TCDD-induced proteasome-mediated degradation of AHR [
To examine the effect of AHRR loss on AHR transactivation, we generated immortalized MEFs from wildtype (
We next determined if AHRR repressed AHR transactivation in the absence of TiPARP and
AHRR has been previously described to undergo nucleocytoplasmic shuttling, with an equilibrium favoring nuclear localization [
We next determined whether GFP-AHRRΔ8_Δ49 and GFP-AHRRΔ8_Δ100 were able to repress AHR-dependent CYP1A1 reporter gene activity in transfected HuH7 cells. Both GFP-AHRRΔ8_Δ49 and GFP-AHRRΔ8_Δ100 failed to repress CYP1A1 reporter gene activity (
The pcDNA-AHRRΔ8 (delta exon 8) and pcDNA-AHRR were generous gifts from Dr. Mark Hahn (Woods Hole Oceanographic Institution, Wood's Hole, MA, USA). Both of these plasmids have been described elsewhere [
MCF7 human breast carcinoma cells were purchased from ATCC (Manassas, VA, USA). HuH7 human hepatoma cells were a kind gift from Dr. Jan-Ake Gustafsson (University of Houston, Houston, TX, USA). The derivation and characterization of the
For the reporter gene assays, HuH7 cells were transfected with or 500–1000 ng pcDNA-AHRR or 5–100 ng pcDNA-AHRRΔ8 or 100 ng pcDNA-TiPARP [
MCF7 cells were seeded in 6-well plates 24 h prior to treatment with 10 nM TCDD for up to 24 h. MEFs were seeded in 6-well plates at a density of 100,000 cells/well. The following day cells were treated with 10 nM TCDD or DMSO for 24 h. RNA was isolated with Aurum Total RNA Mini Kit (Bio-Rad, Mississauga, ON, Canada) and reverse transcribed using SuperScriptIII (Invitrogen Canana, Burlington, ON, Canada). QPCR was done with 1 μL of the cDNA synthesis reaction using SsoFast EvaGreen SYBR Supermix (Bio-Rad). Primers used to amplify human AHRR mRNA were 5′-CCCCGCCCTTGGAGACAGGA-3′ and 5′-AGTACTCGGTGGGCGTGCCT-3′. Primers for human CYP1A1, CYP1B1 and TiPARP mRNAs have been previously described [
Whole cell extracts were prepared from transfected cells treated with 10 nM TCDD or DMSO and proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked in 2% (
MCF7 cells were plated in 10 cm dishes in culture media at a density of 3.5 × 106 cells/dish and treated with 10 nM TCDD for 45 min, 6 h and 24 h and ChIP assays were done as previously described [
MCF7 or HuH7 cells were seeded onto glass cover slips in a 6-well plate (3.5 × 105 cells/well). HuH7 cells were transfected with 100 ng of GFP-AHRR_Δ8, GFP-AHRRΔ8_Δ49, GFP-AHRRΔ8_Δ101, or 500 ng of GFP-TiPARP using lipofectamine 2000. After 24 h, cells were fixed with 4% paraformaldehyde, permeabilized with 0.4% Triton X-100, and mounted onto slides with Vectashield mounting medium containing DAPI to stain DNA (Vector Laboratories, Burlingame, CA, USA). GFP-expressing cells were visualized using a Zeiss AxioImager M2 epifluorescence microscope and images were captured with a Zeiss AxioCam MRm camera and Zeiss AxioVision software.
All data were presented as means and standard error of the mean. A one-way Analysis of Variance (ANOVA) followed by Tukey's multiple comparison tests was used to assess statistical significance (
In this study, we compared the ability of AHRR and TiPARP to repress AHR activity and regulate AHR protein levels. AHRR and TiPARP exhibit a number of similarities including that their expression is increased by ligand activated AHR, that they directly interact with AHR, and that increased ARNT expression does not affect their ability to repress AHR [
The authors thank Mark Hahn (Woods Hole Oceanographic Institution, Wood's Hole, MA, USA), Jan-Ake Gustafsson (University of Houston, Houston, TX, USA) and Kevin Gardner (UT Southwestern Medical Center, Dallas, TX, USA) for providing materials for this study, as well as all members of the Matthews laboratory for their help with the preparation of the manuscript. This work was supported by a Canadian Institute of Health Research (CIHR) operating grant [MOP-125919], a CIHR New Investigator Award and an Early Researcher Award from the Ontario Ministry of Innovation to J.M. and by the Leibniz Association-SAW Competition (SAW) to H.W., I.F. and J.K. IF is a member of the DFG Cluster of Excellence "ImmunoSensation".
The authors declare no conflict of interest.
Laura MacPherson, Shaimaa Ahmed, and Laura Tamblyn performed the experiments. Jean Krutmann, Irmgard Förster, and Heike Weighardt contributed reagents and materials. Laura MacPherson, Shaimaa Ahmed, and Jason Matthews designed the studies and wrote the paper.
Time- and TCDD (2,3,7,8-tetrachlorodibenzo-
AHRRΔ8 or TiPARP repress CYP1A1-regulated reporter gene activity. HuH7 cells were transfected with pCYP1A1-luc and increasing amounts of (
TCDD-induced recruitment of AHR, ARNT and AHRR to
Effect of AHRR or TiPARP knockdown on AHR regulated genes in MCF7 cells. (
TiPARP, but not AHRR knockdown increases AHR protein levels. (
Sub-cellular localization of GFP-AHRRΔ8, GFP-AHRRΔ8 variants and GFP-TiPARP. GFP-AHRRΔ8 (green) was localized in the nucleus (blue) of HuH7 and MCF7 cells (
AHRRΔ8_Δ1-49 and AHRRΔ8_Δ1-100 do not repress CYP1A1-regulated reporter gene activity in HuH7 or MCF7 cells. (


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Thyroid Disorders Message Board
new here can you tell me what a what if you have a TSH level of 125 means
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