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I think that every person in her/his life gives apologies almost all the time. Maybe some apologies are not really important like when you hit somebody on accident or something like that, but there are some apologies that are more important. For example, when you hurt somebody with intention, or when you hurt somebody feelings. But if you give an apologies because you are sorry these is not going to be really important.
When I was younger, I gave a lot of apologies to my parents, because some times I didn't want are obidient or because I didn't want to do some choruses in my home or something like that.
I think that when you give a really good and sincere apology is very important, well for me it is, because almost all the time you can fix or solve many problems that maybe if you donít give an apology it will be probably worse.
Abortion has been discussed for a long time in the world, and this is a serious problem everywhere recently, because medical reports have showed that the abortion rate is increase every year. Abortion means to expunge of the fetus from the womb during the first 28 weeks of pregnancy. People can decide to terminate the pregnancy when the baby has serious congenital disease or the pregnancy will endanger the mother's life. Although abortion has become legal in many countries and most of the American states, in my opinion, I disagree that women have the right to abort the baby except for the special reasons that I have explained before. Abortion should be illegal for two reasons.
The first and most important reason to limit abortion is that the behavior is murder! This was formerly a crime. In a medical textbook that I had read in the nursing school, it was written, "We can use the ultrasound to hear the fetal heartbeat at 64/7 weeks." the baby has been a life since it has been just like an egg's size. How can people be so cruel to kill the little life? For example, a famous news story in Taiwan's newspaper told about a mother who didn't want her pregnancy, because she wanted to divorce her husband. She put a sharp-pointed object into her vagina to cause the baby to be miscarried in the toilet, and threw it on a garbage bin. She was accused of murder. This is a very ruthless truth. I couldn't understand why the mother could kill her own baby by herself!
The second reason to support the idea that abortion must be illegal is that sometimes abortion will hurt the mother's health. Abortion is one kind of operation that has inherent risks. The Taiwan Adventist hospital's Gynecologist-Obstetrician chief Dr. Wei said "Improper abortion will cause a high risk of infertility in the future." One example happened in our hospital 2 years age. A patient did the abortion operation in a small clinic. The unsuitable operation performed on her uterus caused her to bleed heavily. Therefore, the doctors removed her uterus to save her life. She was only 23 years old, and this was just her first pregnancy. She could never have a baby! It's so sad for a woman; a small mistake made all her life a big regret !
Although many people advertise that abortion can provide a good method of birth control, because the population is too crowd in the world. It's not human sympathy! You shouldn't end lives in order to perform birth control. Every people needs to consider the problem before she do the behavior. There are many contraceptive measures that we can use for birth control. For example, both oral pills and condoms are safe ways to do the contraception, and those are more economical than abortion operation. People don't need to choose an expensive way instead of a cheap way.
In conclusion, we know that abortion could cause danger for the mother and fetus, and sometimes it can result in death. As a result, abortion should be limited to protect some people's lives. Everyone should have the right to live in the world no matter their sex, race, and age. It's so wonderful to see a new life born!
In this time, when we find a lot of changes in lifestyle newly-married couples are the most affected. Maybe when they were engaged, they were planing how many kids they will have. In the same way, they draw plans for their future. For example, if they are very young (between twenty and twenty-five years old, or before), and they are also studying, they may think about birth control. This control could be an option until they decide to have a child or many children; but it is not always an effective method. To have a baby is not a game, but it is an important decision to be parents. In this case, they should learn about that.
In my opinion, this topic is very personal and depends on the couple. Only know the situation that they are in . Personal, I LDS and know the importance of our lives, so I am pro-life; but I can understand how the people feel when they don't have enough resources and instruction.
There are a lot of opinions about this topic that we can find if we ask some people. The answer depends on age in the majority of the situations. For example, when a woman is very young, it's possible that she is afraid of pregnancy and the risks of being a young mother without knowing herself and her body. As a consequence, she doesn't like to make an appointment with a gynecologist and have pregnancy check ups.
Another reason that people give for is the financial support. In particular, if they are working and studying. Specifically, in the case they haven't resources and time for care of their child.
Other people say that the future is very important for the couple and their futures children. For example, they should get good jobs and finish their education for a better financial situation.
The final reason for many is that people have the opinion that the couple should to take the necessary time to know each other for a better relationship. Also, if they very young and don't know the efficient method. For example, Billing's method, or another when they had one child, as breast feeding administration (95% effective until six month by technique). There are many natural resources that will make an amenhorrea in the woman.
In the end, is necessary to know the situation and speak about this topic. It's the responsibility of parents to speak about it when their children are teenagers, and also all health professionals. The decision is personal, but needs help from people who teach and explain the topic for the best choice.
Always when I read the word "abortion", the floor is going to move for me. In fact, I can't understand as the future mothers have take a wrong way, namely when are teenagers. I think deep inside where are their mothers for support or for to guide them; meanwhile to take an appointment with the doctor.
I read the essay "Abortion: A Polemic Question" in the Cultural Comparison page, and here impacted to me as in Canada is not a crime, it's absolutely legal. As a consequence a baby is not considered a "human being" until it is born. Of course, not all canadian people are agree with this code. This some people think that something should be changing in law.
Mabel, from Brazil, who wrote the essay compared her country and named the national code that protects the baby. Also in my country, it's the same. For example, on March 25, 1999 Argentina celebrated the first "Day of the Unborn Child". Under our Constitution and our civil legislation, life begins at the moment of conception. The interruption of pregnancy by abortion is an offense against the life of an unborn person. Furthermore, we respect to the Rights of the Child. In the Article 6, (1) says: "States Parties recognize that every child has the inherent right to life". And the Article 24, (d) says: " To ensure appropriate pre-natal health care for mothers".
I'm childbirth educator and for this reason, I'm pro-life; but also I'm LDS and I know the importance of our lives. In this moment is coming into my mind one of my favorites scriptures: "Before I formed you in the womb, I knew you". (Jeremiah 1:5)
A difficult problem for me is about lies. I think to lie to someone is not good. However, many people are lying everywhere. I don't mean only bad lies. I also mean the lie that is called polite lie or white lie. In Japan, many people are using a polite lie. Because using polite lie is Japanese culture and custom. Perhaps I can't decide if the polite lie is good or bad, but in my opinion, I don't recommend using the polite lie.
According to my investigation, there are people who prefer to use polite lies. They say using polite lies is a good for keeping relationships between friends. They praise someone or something too much for me, but they still insist on me using polite lies is good. They also insist on me using polite lies make people feel happy. It makes good relationships between friends. This is the opinion of those who agree to use polite lies.
For example, your friend cooks food for you, and asks you an impression about the food. What will you do? Perhaps, the people who prefer to use the polite lies say, "Your food is pretty good. You are good cook," even though the food is not good. In fact, it would make people happy. The fiends who cooked the food would feel good feelings for the people using the polite lies. Is it a good way? I don't think so. If I use the polite lies like that, my friend would offer me another dish of food. Perhaps I have to finish the dish even though I don't want to eat any more. It is not a fun for me!
On the other hand, the people who don't like to use the polite lie would say "Your food is fine, but it is too salty for me." We don't need to praise too much by using the polite lie. In addition to this, we don't need to say," Your foods is not good." We can say it very softly like my example. Thus, I don't agree using the polite lies.
I want to show you another example. How about the case when people notice that someone was using the polite lie? Perhaps some people would feel that the people using the polite lie to them want to be friends with them. Maybe it makes a little good feeling for them. However, it is not the same for all people. Some people use polite lies even though they don't feel good feeling about the thing. It is absolutely a lie! Therefore, some people would feel worse when they notice someone was using the polite lies than if they don't use the polite lie in first place. They don't want to listen a fake praise. They want to know real opinion you have even though it is not good for them.
However, I don't deny using the polite lie completely, because I think using polite lies is sometimes good. Sometimes, we need to use polite lies on business, because we need to give our customer good impression to us. If we give our customer good impression to us, they will be willing to buy our goods. If we give our customer, they will not buy our goods. In addition to this, they could blame our company for lying to customer. Therefore, using the polite lie like this situation is good. I think using the polite lies in not close friend is also good. If I don't use a polite lie, the friendships will be worse.
To be honest to your friend is a very good thing, I think. The people who agree using the polite lie said it is good for keeping friendships. However, I don't think so. I think to be honest with your friend makes a real friendship. Not using the polite lie makes trusting relationships and good friendships! We don't need to use the polite lie to our friends. We can praise good thing, but we don't need to praise too much. A good thing is a good thing. That is that! This is my opinion about using polite lies.
I don't forbid you to use the polite lies, because people have different opinions depending on their own character and ideas. However, please remember this fact. There are people who don't like to be lied to. If you want to praise, please don't praise too much. You can praise from your real feeling. You also can give advice even though your opinion is not good for something. Please don't forget this advice when you use the polite lie!
I'm going to compare man with woman. By comparing man with woman, I want to know the differences between man and woman. I will write about each gender's good points. However, I can't define which gender is better. Because we have different good points and bad points.
Most different between man and woman is system of body. Man body can make muscle easier than woman body. Opposite, woman body tries to save fat. Therefore, man is usually more powerful than woman. (Of course, there are exceptions.) However, there are good points of woman body that man don't have. Woman body has curves, and it is very sexy and beautiful.
In mental, man and woman both have strong mind, I think. (It is depending on person, not everybody.) However, man is not sensitive like woman, so man can get over from mental problems easier than woman, I think. Of course, woman have good points in mental. Woman has a maternal instinct, so woman is full of love and kind. (Sometimes it is not true, but it is exception.)
In conclusion, man and woman both have difference good points. Therefore, I can't say which gender is superior. We need opposite gender's people. It is essential man and woman in the world. Therefore, we need to cooperation each other. We are even if we have bad points. No one is perfect without God.
When a person gets married, age becomes an issue. We can see unconditional love at the movies and in daily life. Some people a love is blind, so when a person gets married, age doesn't matter, Even if there is more than 10 years difference between the partners. However, last week I saw an entertainment news about movie actor Dennis Quaid (46) and Meg Ryan's (38) divorce news. It was surprising to me. I knew Dennis Quaid and Meg Ryan had been a well-matched couple, so I wonder if why they spilt up. Some people said their divorce's cause is generation gap, so I decided to write about this issue. In my opinion, I disagree with for several reasons.
The first reason to support my opinion is that a generation gap would cause some problems in marriage. It is difficult to feel same feeling and a sense of values. People say love is important, but same feeling and a sense of values is the most important thing. Recently, everything has been changing quickly, so we feel a generation gap with people whose age is only different than ours. For example, my cousin get married to a woman 12 years younger than he was, but now their marriage is on the rocks. Their love was the first love. However, they always quarrel, because they have different a sense of values and hobbies.
Another reason to support my opinion is that in their old age one of the partners would spend their time alone. My grandparent got married 10 years different. When my grandfather passed away in 60 years old. My grandmother spent her time alone for a long time. When their spouse passes away, they have to spend their time longer alone than other couples.
The final reason to support my opinion is that ideal love and marriage is made by both physical and spiritual love. Some people say it is enough to have spiritual love. However, one Korean marriage center said that many couples divorce because of physical love. True love is made both, so physical and spiritual love are both all important things.
In the end, some people say that age doesn't matter when they get married, because love conquers all, and age is relative. However, many people said age does matter. I found interesting thing. People believe differently about the young couples and old couples getting married. For example, it can be a big problem if the girl is 20 and the guy is 30, but it doesn't matter to much though if the girl is 40 and the guy is 50. Anyway, in my opinion, it is better to get married with close age. I think ideal love and marriage is made by both physical and spiritual.
This topic has always been everyone's mouth for a long time. I disagree with it. In ancient times, men governed society, because everything is controlled by physical power. However, futurism expert say 21 century women will govern society. Because in the future, it's more important spiritual thing than physical thing. Actually, men stronger physically than women, but women stronger spiritually than men. I think the best way is men and women mutually dependent.
Not only education specialists, but also ordinary people are confused about whether we should whip our children to teach them or not. Both some people who insist on whipping their children and other people who persist in not whipping them insist that their own way is effective and good for their children. I think every parent loves their children and expects them to be good. Although both opinions are different, both basic thoughts are the same. In other words, it is that both love their children. I want to think what is true help. In my opinion, parents should not whip their children for several reasons.
The first and most important reason is that whipping can hurt children's feelings. Their hearts are very pure and weak, so strong ways can break their pure motives. For example, when one boy was putting in his mouth a piece of gum with the wrapper on, his mother cried out, slapping his hand, "Chew your gum after taking it out of the wrapper!" Mother intended her boy to chew the gum deliciously by taking it out of the wrapper, but after that, the boy didn't want to chew the gum. He was already hurt. I think that mother's behavior is not helpful for him.
Another reason is that whipping doesn't help to make a relationship between parents and their children. When we whip our children, our relationship keeps getting worse and worse. I think that bad things make other bad things, and can't help create good things. A bad thing is just a bad thing. For example, one day, a friend of mine and I quarreled about a trivial matter. I thought that he was wrong. Thus, I told him that because he was not right, he should change his thoughts. However, he didn't change them and came to hate me more. It was not good for our relationship to persuade him to change his thoughts. I couldn't have a chance to talk with him about the problem any more. If I had shown understanding for his idea even though he was wrong, our relationship would be better, and I might have an opportunity to resolve the concern better. Parents sometimes want to change their children in their own way regardless of consideration. It causes hurt in their relationship. As a result, they might not discuss their problem again.
The final reason is that there are better ways to teach children than whipping. We are responsible for developing how to teach them. I like the movie called The Lion King. In the movie, when the baby lion did wrong, his father taught him excellently. He talked to his son about the problem with love. Because he showed his love, his son could understand about the problem without mental hurt and could feel that his father loves him.
In the end, because whipping can hurt children's feelings, and create a worse relationship between parents and their children, it doesn't help children. Therefore, we must find better ways to be helpful for children. We must always focus on our children. We must always show our love. If you think they can't feel your love, you should think again what you would do. We can help our children, and must help them heartily.
I don't think that men are superior to women. Although what men are supposed to do differs from what women are, we can't tell which is more important. Moreover, we can't tell who does their work better. We can only know that men's jobs and women's jobs are different and both jobs are necessary for us. In addition, we can recognize that men are superior to women in the part of physical exercise and work, but women are superior to men in the part of sensitive and emotional things. This reason has us not insist that men are superior to women. As a consequence, we must learn not how they are different but how they cooperate. I don't know what is more important, but I know both are necessary.
A difficult problem for teenagers today is deciding between getting married or living together. Society is getting more and more liberal. Teenagers do many things without commitment in relationships. Many people think that it is correct. However, in my opinion, getting married in order to live together is very important for several reasons.
The first and most important reason to get married is that the couple must take getting married seriously. This proves to the couple that they are sure what they want to do. This decision gives a deep relationship to the couple, and they have more possibility to be successful. When I asked about getting married to my friend David, he said. "Marriage is very good. Without marriage men are lost, and they can't progress. Marriage is very challenging. My determination to get married was to show commitment to my wife, both husband and wife must work very hard to keep the marriage healthy, but marriage is worth it". I agree with my friend. I think that it is the right way to think.
Another reason to get married is to give a good example to sons and daughters. I would like to invite you to think about this. What kind of family could be without getting married? One of most important things in a family is love for each other. Now, is it credible without been married? My father said. "Marriage is good, because you feel happiness with your wife. Your wife becomes your best friend, and she becomes the partner for life. The best part is when you are married and have kids is a blessing to you. The longer you are married, the better your marriage is".
The final reason to get married is that it is approved by all kinds of religions. They teach very good things for their members. One of the most important things is to get married and united as a family. If you believe in God you can find the truth by reading the scriptures. For example in the Holy Bible, Genesis says. " Therefore shall a man leave his father and his mother, and shall cleave unto his wife; and they shall be one flesh. And they were both naked, the man and his wife, and were not ashamed". I believe in this. I think that women and men were created to live together under the name of marriage.
In conclusion, we can find many couples that prefer living together without being married. As a consequence, we find broken families with children without parents able to give them a good formation for their future. For these reasons, to get married, live together, and have a good family is the best thing that we can do in this life.
In my personal opinion this topic had been changed through of the history. Long time ago men had all authority about of the decisions. For example, they built everything, they brought food for the family, and they worked outside home. Only the women could be in home and keep the children. Now it is very different women can work anywhere. Women can build houses. They also work in the government. In many families the woman brings all stuff necessary for her family. I think that there isn't any difference between men and women now. I like to see women to be independent. But I don't like that they lost their sense of maternally.
I want to write about the education of children before they enter an elementary school. One of my roommates believes that mothers can teach their children at home. She was taught many things by her mother before she entered an elementary school. Her mother also taught all of her children, and they became good students in their school. I agree with her. In my opinion, it is best that children are educated at home before elementary school, and they don't need to go to kindergarten. I asked some people about my opinion. I got some agreement, but I also got some objections. I will argue against those objections from my experience.
The first objection was, if children don't go to kindergarten, it becomes hard for them to make friends because they don't have friends at home. One person said, "There are many children in kindergarten, so children can make friends easily, and they can play with other children different from their brothers or sisters." I disagree with this opinion. I didn't go to kindergarten, but I have always had friends in my life. We have some exceptions, but my brothers have also had their friends. My youngest sister went to kindergarten. However, it is hard for her to communicate with other people. This shows that children don't always need to go to kindergarten to make friends.
The second objection was that children have teachers in kindergarten, and teachers can help them. They help children prepare to go to elementary school. For example, they teach how to be in a group. They also can teach students many things. However, I think mothers can take teachers' places for their children before elementary school. I learned music, English, Japanese, reading and writing, easy mathematics, and the gospel from my mother before I entered an elementary school. This shows that children can learn many things in their own home before elementary school.
The third objection was that, children can learn a lot of things which are different from their home or elementary school in kindergarten. Children learn playing games. They also learn to make friends and build relationships. However, My mother also taught me some playing games. There were many children in my neighborhood, and I was able to learn to make friends and build relationships. I also learned those things in church. This shows that children can learn to make friends and build relationships out of kindergarten.
From these examples, I think that kindergarten is not always needed. However, sometimes many mothers must work for their family. I think the children of working mothers need kindergarten. Except in the situations when mothers must work whether they like it or not, I think they had better teach their children at home.
I have some opinions about when I will become mother. I have heard about many kind of mothers. Some of them are very kind and good mothers, but some of them are too kind or too cold for their children. I was also looking at my mother, and I thought a lot about mother and home training of children.
I want to teach my own children with my hands before they enter an elementary school. I don't want to enroll them in kindergarten, especially, before they become three or four years old. I also want to become their good example, and teach them with the gospel.
I want to become a mother like my mother. She is a good example for me. She taught me many things before I entered an elementary school. She always loves her children, and she always helps them whenever they need. Especially, she taught me many things to follow the gospel.
Maybe my opinion is very different from other people. For example, many Japanese families send their children to kindergarten, and most mothers don't teach their children. I think they love their children, but sometimes some mothers are violent. I don't want to become like them.
Do you have a pet? If you have a pet, have you ever felt loyalty from your pets? Do you think that they are like humans? Most people who have had a pet before agree with these questions. Each of these peoplewith whom I talked with shared some kind of example with me. A few people disagreed with me, perhaps because they had never owned a pets. I think that when people keep pets, they can understand what I mean about animals' loyalty.
My pets are like a part of our family. When we are eating something, they also come to our table and sit there. Moreover, my family and pets watch TV. I think if we spend time with animals, they start to act like my family. For example, our cat looks like my mother, and our dog looks like my youngest sister who always gives him food and takes care of our pets. They are much the same characters. If someone gives these food or loves them, they obey them. It is an interesting relationship.Vafortunately, they don't act like me. I was working a lot, so I didn't have a lot of time to play with them.
Second, a lot of people said that if we show love to our pets, they could understand it. Also they want to be loyal to us. For example, one day our cat brought a bird for us. We don't need a bird, but she wanted show to loyalty. Even though my mother was upset about her action, our cats still bring something for us. For an other example, when I was crying from sadness, our pets came to me. They cheered me up. I held them; both of them licked my face. Animals don't care about money, position, or looks. They just love us. I also love them. I appreciated that my pets could understand my feelings very well. They are my best friends.
Third, pets can understand about our talking. For example, when our dog hid our shoes, we got angry, and we were preaching to him that it is a bad thing. Our dog was sulky. He didn't look at us. I felt that he looked like a human child. And also, when we did training to our dog about "sit down" or "house", they try to understand what we are saying. At first, they couldn't understand our talking, however day by day they learn our saying. Maybe, pets can figure out from our voice and intonation. Sometimes they are very smart!
When humans and pets spend time together; we can have a strong relationship with each other. The pets show loyalty, and we can love them more. When human children are young, we should keep a pet. Children can learn about love, kindness and service. God gave us animals, so of we can help each other. How great animals are! Now, we call them "companion animals." I want to stay with my pets. They bring us happiness. The sadness is that their life is very short. We can learn about life from them. I appreciate their example. I believe that I will able to meet again with my pets in Heaven.
The discussion of abortion continues in the entire world. This means it is a sensitive problem. In addition, the thought about abortion is different in each country. Almost all countries prohibit abortion. However, my opinion differs from that for two reasons.
First, we shouldn't give birth to a baby if we don't want to be pregnant. We can see the women that are raped. As a result, they sometimes become pregnant. If they bear the baby, the life of the baby is dark. The reason is that the baby doesn't know the father. Another situation is when a minor becomes pregnant. She doesn't have any preparation to bring up a baby. For example, in Korea, babies that are conceived through rape or by a minor go to an orphanage.
Second, the most sensitive situation is when a woman is pregnant with a handicapped baby. Personally, I don't think that handicapped people are useful, because these people are children of God, too. However, society is very cold when handicapped children grow up. Moreover, the family maybe hurt. For instance, a family I know, the Kims, has a handicapped first child. They didn't abort, even though they knew it was a handicapped baby, because they thought this behavior was a crime. However, they have many problems from their baby. The biggest problem is that the baby has been hurt by society. For example, the baby couldn't go to any kindergarten or school. Instead of that the parents want the child go a school for the handicapped. However, the entrance fee of school for the handicapped is almost triples the amount of their salary for one month. In addition, their welfare payment is very small. Even worse, they became pregnant with a handicapped baby again. As a result, they decided to abort. I think that their choice is inevitable. Some anti-abortion people say, "Abortion is murder." However, if they are faced with two examples like the above-mentioned, their choice is abortion.
In the end, is we prohibit abortion, we need more reliable contraception. However, if contraception fails, we need to make a big decision. Parents must make the decision. However, we must also consider the life of the baby. If we don't make good surroundings for the baby, this is the fault of the society that prohibits abortion.
I agree with this topic. However, I only recognize about physics. Men are superior to women in force. However, the women haven't been given the opportunity. As a result, the number of famous women is less than men. If we have mire opportunity, we can develop big achievements. I think that it is so funny to discuss the ability of inborn ness. Humans are all the same. Of course, there is a different ability in each person. However, there isn't ability in each sex.
We are living in a modern world where the relationship between countries and people from different countries are getting closer and closer. In this time it is easier to travel because we can enjoy big planes or large boats which can go very fast to every part of the earth. Also, they can make our trip relaxing and wonderful. For this reason, it's easier to know people from different cultures. A new world is open in front of our minds. Many people are taking advantage of this situation and they are getting married to others from different countries. In my opinion, it's an amazing way to build a better world.
I have several reasons for agreeing with this situation. First, even if we are different in many ways, we are all human. Therefore, we all have the same feelings. For example, my grandfather Lucio left Spain when he was 21. He went to Holland to get a better job. In Holland he met a nice Dutch girl named Martha. Time passed and they fell in love each other. In the beginning it was a difficult situation, because my grandfather could not speak Dutch and Martha could not speak Spanish. Nevertheless, they kept loving each other. Two years later, they got married in Rotterdam, Holland. I can still remember my grandfather telling me some stories about his engagement. "Even when our cultures were queer," said my grandfather, "we learned a lot together; yet, the most important thing we learned was how to respect each other." Most people would think that it's a crazy situation. How could they communicate with each other without speaking the same language? In my opinion, they found the perfect communication: the communication of love.
Second, sometimes you can understand a person from different country much better than a person from your own country. My best friend is from the U.S.A. I met him when I was serving a mission for The Church of Jesus Christ of Latter Day Saints in the Canary Islands in Spain. One year after I finished this mission he called me and told me that he was going to get married to an Argentine girl. Months later, I had the opportunity to meet with them at their wedding in Spain. There, I could tell they were a perfect couple. At this time I'm living in the same city that my friend and his wife are living in. As a consequence, I see them almost every day. Thus, one day I went to their house and I asked them several questions about this topic. One of my questions was, "Have you had any problems in all this time since you got married?" He looked at me and answered sincerely, "In the beginning, it was a little bit complicated, because my family and my wife's family didn't speak the same language; nevertheless, as a couple we have not had any problems so far. I have found in my wife a lot of things that I could not find in another girl, even from my own country." Therefore, even though they are from different countries and different cultures, they have come to understand each other easily; with comprehension and love they are building a happy home.
My final reason about marriage between people from different country is very simple, yet very strong. Love is the most important thing. My grandfather told me once: "When I met your grandmother, I thought it would be too difficult to get married to her because we were different in some ways. Nevertheless, one night I looked at the dark sky and I saw a lot of bright stars shining strongly in the middle of the darkness. Even though they are very far from one another they seem very close. In that manner love works-making closer what is far away." Although I was very young when my grandfather told me this thing, I can still remember he had a very happy life. He and his Dutch wife found happiness together.
In conclusion, to find a person from a different country with whom you might live may be difficult, yet not impossible. In my humble opinion, we can't close our minds. We don't know our future. Maybe it's full of surprises. Also, we can't know where our perfect future husband or wife is living right now. Perhaps our only mission is to find him or her and be happy when we do. It doesn't matter if we need to climb the highest mountain or if we need to get down to the deepest sea. Indeed, love is the most important thing. As my grandfather told me once, "Love may make bright our dark skies."
I read an article about abortion. This topic is very interesting because many countries around the world have different opinions. For example, in my country, Honduras in Central America, abortion is not good, but, like in other countries, some poeple do it illegally. In my opinion, abortion is not good, because women that are pregnant have a life in their uterus. It is important to respect other people's life.
Abortion is a very difficult topic to talk about. in some countries, abortion is legal. I read that in Canada it is legal, but in my country it is illegal. As a consequence of that, in my country are some illegal clinics, or women who want to abort their baby use other risky methods to do that.
On the other hand, there are many women who want to have babies, but they can't have them for some reason or another. It is very sad how people can kill a little person. It is a very cruel situation that happens very often in these days.
I do not agree with abortion. I think there are other ways and not only abortion. If the person doesn't want the baby, they can give them up for adoption. In this case, the children can have a family who loves and takes care of them. In my case, I want to have my babies, love, and teach them good things for their lives.
The purpose of this topic is to learn what people think about kissing their best friends. I questioned many people about this topic and I got different opinions. The majority of the people disagreed with this question. I chose this topic because I would like to know if people have been in this situation before and that is usually or unusually. Also, how much is important of kiss for them and how they value the friendship through two persons of the sex opposite and what can happen later.
Most of the people answered that they would not kiss their best friends for various reasons. First, a kiss is sacred. Second, a kiss is romantic and means something very special. Third, they do not want to confuse friendship with romance. In addition, they value the friendship too much they don't want to lose a best friend for a kiss. Is not necessary to kiss your best friend of the opposite sex to show you love because if you steal his or her kiss you are not respect his intimacy and you are broke his space and you don't know if he is going to be angry in this situation. In conclusion, they would like to keep their friendship forever, because to have a good friendship with someone is very important in their lives.
In contrast, some people believe that it is a good idea to kiss and begin a romance with your best friend. Friendship is the first step to a good romantic relationship. A friendship can grow into a good romance. Therefore, your best friend can become a good husband or good wife. Otherwise, sometimes this situation don't happened because the other person has feeling such as a good friend for this person and they can broke up their relationship, that not always pass positive but they want to run the risk of that situation
In my personal opinion I do not agree with kissing my best friend, because he is such a special person in my life. Therefore, we should respect each other because the friendship is very important. However, sometimes we can fall in love with our best friend. If that happened to me, first I would to tell him about my feelings. This way we would not damage our friendship, and if he felt the same way we could move the relationship forward with trust in each other.
Living in separate cities after got married is a very hard thing to do; particularly if a couple just got married. Although it is hard, sometimes we have to do it in order to get improvement in our life. I am from Indonesia, and I am studying English in the United Stated. Moreover, I can't bring my wife with me because of some reasons. For instance, she has a good job in my country. In addition, we need to save our money for the time when she is coming to U.S. while I continue to study to graduate school. Therefore, I have some reasons why I agree that sometimes it is necessary for a couple to live separately for a temporary period of time.
The first reason is if it is the best thing to do for their future. For example, a husband has to move to another city because his company suddenly promotes him for a better occupation. However, his wife can't go with him at that time, because she still has something to do, such as working, business, sick and still on doctor checking, and so on. I think it is all right to leave her only for a period of time like 6 months or a year, or until her business is done.
The other reason I agree with a couple living in separate cities is if a husband wants to get a job in another city. In other words, they want to improve their living. For example, a husband had to go to another cities because some company calls him for work. However, he can't bring his wife with him for some reason. For instance, the wife is working and probably need more time to prepare the moving, so that everything will be settled before they completely moved. In addition, perhaps they have never been in that city, therefore the husband go first to recognize it thoroughly.
The last reason is nationality reason. For example, a husband is probably in army service. He has to go somewhere for several months for training, or for war. It's supposed to be impossible to bring his wife along. I think she had better wait patiently until he comes back, so that the husband can do his duty as well.
Those were some reasons and examples why a couple was supposed to live in separate cities for temporary and for specific reasons. Sometimes we have to make a sacrifice, so that hopefully we will get better in the future. People prefer to avoid a sacrifice to reach some goals. However, they have to make it in order to get some improvement in their life.
Should people get married to people from other countries? I know an international couple. They are my good friends. The man's name is Reed, his wife is Shinobu. They seem like a very good couple, I think. Reed is from Hawaii, and Shinobu is from Japan. I think it's no problem when I watch that couple. This is my answer for these question. There are two reasons why I believe this way.
First, I think love is more important than nationality. My opinion is that it is only important for me that my wife has good morality and good thinking, and always cares about me. My friend Kinue disagree with me, I don't matter. Reed and Shinobu said to me, "Actually, it's no problem for us, but normally there are many problems before marring. Sometimes some couples can't get married, even if they have strong love." So then, the most important thing that I thought is that the couple has great mind, it's like comfortable, good morality, each other. They are always honest.This thing is important. This is the most important for all human being. I believe that. And Reed's couple is Mormon, so they are very kindly and always honest.
The next reason is couples can know another culture. Sometimes this thing is possible to make some problems. Especially, different language couple is difficult. Because they can't understand what they are telling each other And in addition, this is really my personality reason, it's I like face and atmosphere and independent mind about American girl. But Kinue's opinion was different from mine. That was "Personally, I disagree with you. Because they don't know how to do housework. Most of the American women don't care about their husband. Reed's wife is not American, so I asked my American girl friend about that opinion. Her father is American, and her mother is from Chile. She said some couple is so that, but most couple is not about caring about their husbands. I think so, too. But she said most of American women don't care about housework.
Another question, "Should the couple live in the man's country?" Reed does so that. Now they are living in Hawaii. I don't know why about them. But I agree what the couple live in man's country. Because in our culture and customs, usually we do so that. Now I think this "the couple live in man's country" is maybe only my personal opinion. I thought when I read the other person's answer in the virtual class discussion. They didn't write so that like mine.
As a result, at first I thought I could get married with another country's woman. After interviewing, my opinion little changed. It's that normally, it's not easy about international marring, but if I can find the woman who can speak Japanese, and she is much my reason, maybe I'll get married her. But it's difficult for me now. The wall of language is the big problem. So now, another country's women are good as friend, I think.
Many mothers from all over the world want to go to get a career, and find a good job to make money to help increase their families living standard. However, this has problem; who will taker care of their children? From my own opinion, I think mothers need to stay with their children ; because children need love from the mother. Also, it is their responsibility to train their children in their home. In contrast, some mother want to have a career, and find a good job. Sometime it is very difficult to do, but I think if universities, and colleges have a care center for student's children, mothers can stay closer their children.
Some mothers choose to stay home to teach their children, and spend a lot of time with them. This is the best way to take care: with love to teaching with love. For example, I have a friend who have ten children, and I had the opportunity to live with them for several months. I learned a lot how Christine teaches her children, and she spends time with each of her children. She is very busy every day. Christine is very happy to stay home with her children.
Recently, on newsnet.byu.edu, there was an article about women discussing the importance of having mothers stay at home by Tara D. Mckinney. There was a story about the Marck family; Jane Marck chose to stay home with their seven children and train her children, because she felt it was her responsibility. She needs to spend quality and quantity time with them. Jane has the opportunity to work in home with her children every day. I think the most important thing is the father of the family has to support when mothers choose to stay home and take care their children.
Many mothers today are very young, and they want to get a career, and some need to find a good job because they are divorced. They have to work to provide for the family. It is very difficult for mothers to stay far away from their children, but I think if universities, colleges, and some companies have a day care center, mothers can stay closer to their children.
I know this project would be difficult to organize, and it would expensive. However, the universities, colleges, and companies can find people to donate used toys and books. In addition, mothers could search for sponsors to pay a small enrollment fee. Also, mothers can help work in the day care center. For example, I have a sister who has 3 children, but she is divorced she needs to work hard and her children need to stay in a day care center. Some time she work in the day care center to have the opportunity to stay closer her children, but some time she felt sad because she can't spend time with her children.
I belive if universities, colleges, and companies created a day care center, mothers could study, and work with more tranquillity and they could be closer their children. Mothers could get an education and could find a good job, get experience; make money, to pay their own way in life. These are some reasons why universities', colleges' and companies' need to create a day care center.
I hope that the universities', colleges' and companies' administrations will consider this proposal carefully. It's time to do something for mothers who have problems. Finally, mothers can choose to stay with their children, also mothers can get a career in the future help their family, and women could feel self-confident.
Parents raise their children sometimes with rules. The children have to do that parents are teaching in their home. For example, I saw friends who taught their children how they could clean their rooms, and to come home early when they are at a party and to get up early to help before going to school. Moreover, parents have time to take vacations with their children, and tell stories about when they were children. However, parents are teaching their children about good, bad, right, and wrong. Those are very important for them, because today it's not easy to raise children. The world is growing more bad then good, so parents need to teach their children about what is going on in the world. I think parents need to be the best friends for their children, and take time to speak to them with love. Otherwise, their children not going to making good choices. Also, I think becoming a parent is not easy, but if we pray, I know Heavenly Father will help. If some day I have children, I will teach them with love, and I would like to become their best friend. I would like to give a good example.
I disagree about men being superior to women. I think women can have the same careers that men have, and women can work hard like men do. Sometimes women can help men to become better, because women are more sensitive than men. I think women work hard when they have children,and working outside the home is not easy for women. Men are not superior to women, but in some jobs they are stronger They can build a house or carry something heavy. However, women could take care of their children better than those men. I think it's a beautiful part of creation that we can become good fathers and good mothers. In conclusion, there is not one who is superior, but each one has a mission to do on the earth.


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They are swayed by the promise of jobs in another city or even another country. Perhaps they are promised work in hotels, constructions jobs, or as nannies who "love to travel." They go off with the person who recruits them. When they try to leave, they are told they can't go; they have a travel debt they must pay. They are warned that if they try to leave, their families back home will be harmed. They are forced against their will to work as prostitutes or perform backbreaking acts of physical labor. They are modern slaves. Continue reading A Primer on Modern Slavery – Lunch w/TED
Author: Lauren Myracle
The Placebo Effect and The Imagination- Lunch w/ TED
Apropos for April Fool's Day, today's Lunch with TED clip from a 2009 TEDMED features magician Eric Mead marveling at the effectiveness of placebos in medicine and demonstrating the power of imagination over reason. Be forewarned: this clip is not for the faint of heart. Continue reading The Placebo Effect and The Imagination- Lunch w/ TED
The Suprising Spread of "Idol" TV in the Middle East- Lunch w/TED
Reality TV in the United States has a poor reputation. And frankly, it often deserves it. After all, some of this year's biggest reality hits include raunchy, mindless fare like "Jersey Shore," "The Real Housewives" franchise, and "The Hills." As for less smutty, more family-friendly shows like "The Biggest Loser" or "American Idol," well, they're not exactly high art. Continue reading The Suprising Spread of "Idol" TV in the Middle East- Lunch w/TED
J.J. Abrams on Story, Technology, and "Mystery Boxes" – Lunch w/ TED
Our "Lunch with TED" feature is back—and here to stay. (For the uninitiated, see Dustin's original TED post here.) To commemorate this momentous occasion (and, frankly, the return of Lost, now in its sixth season) I chose to highlight a TED Talk by one of my favorite filmmakers—J.J. Abrams, a producer of hit films and TV shows including Lost, Alias, Fringe, Cloverfield, Mission Impossible III, and Star Trek.
In this TED Talk filmed in 2007, Abrams explores the relationships between mystery, story, creativity, and technology. In stories, he explains, the withholding of information is often more interesting than the revealing of it. Mystery and possibility tantalize us and keeps us coming back for more.
This, in part, explains my household's addiction to his television shows. After all, half the fun for fans of shows like Lost or Fringe is spending spare moments between episodes puzzling over what happened in the previous show and speculating about what's coming next. Shows like these have the singular ability to not only provide entertainment, but wonder—a rare thing in a rapidly disenchanting cultural landscape.
Our ever-evolving technology is a great boon in as much is it helps to tell stories that create wonder. Though we often over-consume and abuse the gifts of technology (Is there ever a moment when our devices cease their whirring?), its rapid evolution is a gift for storytellers in a variety of mediums. It has leveled the playing field so that essentially anyone with creativity and gumption can easily produce quality work.
There is no excuse for you, then, young artist. In the words of J.J. Abrams, "Go make your movie!" '
On Overcoming Writer's Block
After staring idly at the white screen for a few moments, your brows furrow. You type out a sentence. It is terrible; you must erase it. You notice your palms have begun to moisten and your intestines are all in knots. Disconcertingly, this process repeats itself until the worst has happened. You have opened all the drawers of your mind only to find you have nothing to say. Continue reading On Overcoming Writer's Block
On Reading the Bible
In an essay at Modern Reformation, David Nienhuis presents the rather bleak case that Americans are biblically illiterate. What's worse, their Evangelical counterparts are little better. A professor of New Testament Studies at Seattle Pacific, Nienhuis begins his survey of the Christian Scriptures course with a biblical literacy quiz which consistently results in a 50% class average—in other words, an "F." Continue reading On Reading the Bible
Finding Flannery
Flannery O'Connor famously claimed that "there won't be any biographies of me because, for only one reason, lives spent between the house and the chicken yard do not make exciting copy."
Happily, Brad Gooch has begged to differ. Continue reading Finding Flannery
Rural Studies and the Death of Main Street
The small towns of America's heartland are becoming an endangered species, argue researchers Patrick J. Carr and Maria J. Kefalas in Hollowing Out the Middle: The Rural Brain Drain and What It Means for America—a lengthy title for a slim and troubling ethnography. In a nation where urban studies and development are hot topics, Carr and Kefalas turn their attention to the rapid depopulation and economic crises facing Main Street. The family farm has all but vanished into corporate agribusiness, and the industrial jobs rural workers take instead pay poorly and have an uncertain future in the current economy. Carr and Kefalas note that some rural researchers have gone so far as to suggest that the answer to such inevitable decline—economically and ecologically—is "to return much of the Great Plains to its original state"—a Buffalo Commons.
What distresses the authors most is that the rural, Midwestern towns containing the "real Americans" politicians try to comprehend every four years have been steadily losing their best and brightest young people to more metropolitan locales over the past few decades. This exodus has caused the median age in these towns to skyrocket, caused schools to close, and created a dearth in civic leadership, economic growth, and medical facilities. As the Heartland's "creative class" invest their human capital in cities such as Austin and Phoenix, the "regional losers—the laggard, blue-collar red states […] find themselves fighting to keep their communities and counties viable."
Carr and Kefalas moved to a northern Iowa town to investigate this phenomenon for themselves. They discovered that students whom they aptly termed "Achievers" left not only because of unappealing employment prospects but because the town expects them to leave, attend college, and accomplish great things—despite the fact that these towns are shooting themselves in their proverbial feet. "Stayers" are towns' underinvested in, low test scorers, those of lower-middle class status who marry right out of high school and find work locally. They enter the workforce little comprehending that they will make the same paycheck at 40 as they did at 18.
While states are scrambling to build campaigns to attract "Achievers" to return to Iowa or Kansas, Carr and Kefalas would urge small rural towns to nurture and invest in the "Stayers"—to equip and update them with technical skill sets utilizing the community college system, to emphasize computer skills, and to generally "raise the human capital of those who stay." They also recommend that there be a "national call to move to sustainable agriculture and green energy technology" to the heartland, and that immigrants be given the opportunity to gain legal status and work in well-regulated industries.
But why should Americans care about the future of small towns in our heartland? After all, in a capitalist system, are there not going to be winners and losers? Carr and Kefalas argue that it isn't that simple: the country, they claim, couldn't properly function without these small Midwestern towns. Our food and other natural resources come from these areas, they say. Do we really want to see the continued propagation of cheap and unhealthy food grown by large corporations? The Midwest is ground zero for sustainable agriculture, as well as green energy. It is one of the best places to develop wind and solar power. The region is also historically central to the health of the nation, they argue, and America is best when unified. We should care about all parts of our country; as we care for struggling urban areas, we should also invest in struggling rural ones. '
It's a Mad (Men) World
Spoiler Warning: If you haven't seen the Season 3 finale and still want to, read no further.
AMC's Emmy-winning, media darling Mad Men wrapped up its third season last week with a bang and a whimper. In the season finale, "Shut the Door. Have a Seat," ad agency Sterling Cooper Continue reading It's a Mad (Men) World
You Are What You Eat…And Not Who You Sleep With
Food and sex have shifted roles over the past fifty or so years, argues Mary Eberstadt in a fascinating essay at Policy Review. Once, social stigma condemned extra-marital philandering. Sex was a serious ethical issue, with serious personal and social consequences. Food, however, was something with few, if any, moral implications. Particularly for a generation with memories of the Depression, one was simply glad to have food at all. Continue reading You Are What You Eat…And Not Who You Sleep With

subset: commoncrawl


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Garnier Fructis Triple Nutrition Strengthening Conditioner Detangle and smoothen your hair. This is infused with the goodness of 3 nourishing oils of olive, coconut and almond. The Vitamin E from these oils helps moisturize the scalp and get rid of the dryness.
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Apply after shampooing from root to tip. Leave it for 2-3 minutes. Rinse thoroughly.
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Brand: Garnier Fructis.
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TMG-L ArchivesArchiver > TMG > 2011-03 > 1300406996
From: Rick Van Dusen <>
Subject: Re: [TMG] Cloud computing
Date: Thu, 17 Mar 2011 17:09:56 -0700
References: <email@example.com><firstname.lastname@example.com>
In-Reply-To: <firstname.lastname@example.com>
I'm in a similar situation to an extent. My main personal computer
doubles as a file/print server, and is in active use on and off 24/7, so
leaving it on is only a slight step. It also allows functions which
can't happen if the machine is ever off. (I do shut this machine down
once a week for less than two hours for backups, and I have to do that
at a time when nobody else is using a computer, which ain't easy.)
As Lee does, I have the computer set to reduce power after 20 minutes of
inactivity. However, I don't put it into full sleep mode due to the
server demands.
Naturally, my networking equipment is also on 24/7. And all computers,
monitors, and networking are connected to UPSs, so I'm not hampered by
power outages.
Other computers, being used much less often, are only on when used, or
from the time they're turned on until the last user goes to bed.
Just to give a more quantitative answer re the power bill impact: At
this moment, with the computer in full use, it's pulling 181 watts
(that's computer, monitor, and three networking devices). That's nearly
two 100-watt light bulbs, but note that this is full usage, not in
reduced power or with monitor turned off (which I do overnight). (I've
done everything I can to have this computer energy-efficient, such as
very green power supply, thermo-controlled fans.)
Our bill takes much more hit from people using the TV and game devices,
and having lights on much of the night. (But that's a whole different
problem and not on-topic. <g>)
Lee Hoffman wrote:
> Rae Jean <> wrote:
>> I am curious -- do you leave all of your computers running 24/7?
>> If so, what impact does that have on your utility bill?
> Yes, I leave all my computers (three desktops and a server) running
> 24/7. Plus a couple of laptops are often plugged in for
> charging. Yes, the utility bill is impacted although not that much.
> Usage on the computers is such that they are being used almost
> anytime day or night. Thus, with 24/7 operation, the extra time and
> expense of start-up and shutdown is eliminated which offsets the
> added costs of full-time operation. All my systems are configured to
> go into sleep mode after 20 minutes which also reduces operational costs.
> 24/7 operation also allows me to do a lot of scheduling of jobs that
> take time to do. Mostly these are backup and security checking and
> most of these are scheduled for times when the system is least likely
> to be used for normal work.
> Hope this helps -
> Lee Hoffman/KY
This thread:
|Re: [TMG] Cloud computing by Rick Van Dusen <>


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(CNSNews.com) - A new poll shows most Americans give Ronald Reagan a higher retrospective job approval rating than when he was in office. In contrast, Bill Clinton's retrospective rating slipped lower than his job approval rating while in office, according to a Gallup poll.
Ronald Reagan's average job approval rating while in office between 1981 and 1989 was 53 percent, but looking back, 73 percent of Americans say they approve of how he handled his term in office.
Clinton averaged a 55 percent job approval rating during both terms in office between 1993 and 2001, but those surveyed today give him a 51 percent approval rating.
According to Gallup, those who remember Clinton most favorably tend to be Democrats and nonwhites and younger Americans. Those least likely to approve of him are Republicans, Midwesterners, older Americans and whites.
John F. Kennedy, who had the highest average job approval rating of the last eight presidents (70 percent) while in office from 1961 until his assassination in 1963, is still the favorite among Americans, with a present rating of 83 percent. More than eight out of 10 Americans say they approve of the way Kennedy handled the presidency.
In the survey, conducted between March 18 and 20, 2002, John F. Kennedy was number one, followed by Ronald Reagan and George H.W. Bush. Gerald Ford and Jimmy Carter received above average scores, while Bill Clinton's score was average, followed by Lyndon Johnson and Richard Nixon whose scores were below average.
Jimmy Carter averaged only 45 percent while in office, but in retrospect, 60 percent of Americans approve of how he handled the presidency. Likewise, Gerald Ford averaged 47 percent during his brief time in office, between August 1974 and January 1977, but he now receives a 60 percent approval rating.
George H.W. Bush's average job approval rating while in office is the second highest of any of the last eight presidents (61 percent), but currently gets a 69 percent approval rating.
Lyndon Johnson and Richard Nixon's approval ratings suffered in retrospective polling. Both did well in their first terms in office, with Johnson getting a 74 percent average when he took over after the Kennedy assassination in 1963, and Nixon receiving 56 percent in his first term.
But respondents now give Johnson a 39 percent approval rating, down 16 points from his term average of 55 percent by the time he left office. Nixon, who received only a 34 percent approval rating in retrospect.
The results are based on telephone interviews from a randomly selected national sample of 1,009, 18 years and older. There was a margin of error of plus or minus three percentage points.
E-mail a news tip to Melanie Hunter.
Send a Letter to the Editor about this article.


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This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
A series of recent studies demonstrated an unexpectedly high frequency of intronic RNA polymerase (pol) III transcription units spread throughout the human genome. The investigation of a subset of these transcripts revealed their tissue/cell-specific transcription together with the involvement in relevant physiopathological pathways. Despite this evidence, these transcripts did not seem to have murine orthologs, based on their nucleotide sequence, resulting in a limitation of the experimental approaches aimed to study their function. In this work, we have extended our investigation to the murine genome identifying 121 pairs of mouse/human transcripts displaying syntenic subchromosomal localization. The analysis
RNA polymerase (pol) III drives transcription from three different promoter types referred to as type 1, type 2 and type 3. Type 1 (only found in 5S rRNA genes) and type 2 promoters (typical of tRNA genes) are endowed with transcription control elements located within the transcribed portion of the genes. On the contrary, in type 3 promoters (such as U6 and 7SK) the regulatory elements are positioned upstream to the transcription start site (TSS). Indeed, the type 3-associated Proximal Sequence Element (PSE) maps between −66 and −46, whereas the TATA box maps around −30 in the case of the prototypical human U6 snRNA gene [
Our first screening
According to our results, more recent studies identified a remarkable number of pol III-transcribed ncRNAs by genome-wide location analysis of pol III machinery contributing to the growing appreciation of the widespread involvement of pol III in the expression of mammalian genomes [
In this work, we extend our PSE/DSE (Distal Sequence Elements)-based approach previously used in humans to the identification of the pol III type 3 transcriptome in mice with the final aim to identify pairs of mouse/human transcripts that display syntenic subchromosomal localization and, possibly, to find experimental evidence of their functional homology. To this aim, we searched for a comprehensive panel of murine pol III type 3 transcriptional units taking advantage of a recent bioinformatic algorithm able to screen the murine genome in search of genomic sequence stretches harboring the functional consensus sequences of type 3 promoters.
We identified 702 putative murine pol III transcriptional units whose analysis
In order to design a PSE consensus sequence suitable for a bioinformatic search of type 3 promoters in the mouse genome, we aligned the PSE consensus sequences of three well-assessed human pol III-transcribed elements (H1, U6 and 7SK) with their murine counterparts. Since these consensus sequences are extremely conserved between the two species, to screen the mouse genome we used the human PSE consensus template together with the other parameters previously used for the search of the type 3 promoters in humans such as: (a) a PSE consensus sequence (TYACCNTAAC, obtained aligning H1, U6 and 7SK PSE sequences); (b) a PSE/TATA spacer (35 ± 25 nt); (c) a TATA box consensus sequence (TATA); (d) a transcribed portion length (350 ± 200 nt); and (e) a mammalian pol III transcription termination signal (TTTT) (
In order to verify the possible correlation between the presence of protein-coding genes and novel pol III transcriptional units, we crossed the number of items in the single chromosomes with the number of protein-coding genes that it contains. We found a significant positive correlation between the occurrence in the same genomic regions of pol II-transcribed protein-coding genes and pol III type 3 transcriptional units that overlap spatially (linear regression coefficient (
Next, we selected the subset of items that map in intronic portions of coding genes and, by using BLAST algorithm, we analyzed their subchromosomal localization. We found that 702 transcriptional units (out of 2333, (30.1%);
This set of transcriptional units was further restricted since the elements were grouped in intronic and exonic transcription units by the means of their localization within the genomic portions of the protein-coding genes using the algorithm BLAST. We found that 61 out of 702 transcriptional units (8.7%) map in exons whereas 641 (91.3%) map in introns (results for each chromosome are shown in
Besides the subchromosomal localization we also tested our collection for possible recurrent functional annotations associated to the protein-coding genes that contain pol III transcriptional units. To this aim, we took advantage of the algorithm DAVID that allowed us to assess the possible enrichment in genes involved in the same cellular processes/molecular pathways and/or characterized by the same tissue specificity of expression [
By comparing the list of mouse intronic pol III transcriptional units with the corresponding human dataset [
Next, we focused on the possible functional homology between human and murine pol III type3 transcriptional units. To test this hypothesis, we selected 38A (human) and 38B (murine) pol III-transcribed RNAs as possible pair with homology of function. 38A is a well characterized ncRNA that maps within intron 1 of KCNIP4 (Potassium Channel Interacting Protein 4) and plays a role in alternative splicing regulation of this protein in brain [
In order to test the expression of 38B ncRNA we measured by Real Time RT-PCR its amount in different tissues obtained from a pool of C57/BL6 mice. Results (after normalization to the heart sample) showed that 38B is poorly expressed in testis, intestine and spleen whereas its synthesis is strongly increased in cerebellum and in total brain extracts, matching with the expression pattern of KCNIP4 protein [
In order to assess if the synthesis of 38B drives to KCNIP4 alternative splicing (thus recapitulating its human counterpart 38A) we analyzed the possibly increased expression of the alternative KCNIP4 variant C in hippocampal neurons overexpressing 38B RNA. To this aim, we generated a plasmid construct harboring the whole 38B transcription unit (hereafter referred to as p38B-EGFP-N1) together with a GFP cassette. The plasmids (and the empty vector pMock control) were transfected in primary cultures of hippocampal neurons and the amount of 38B ncRNA, KCNIP4 variants A and C were measured by Real Time RT-PCR in both samples (
In a previous work we demonstrated that the synthesis of the human KCNIP4 alternatively spliced Variant IV protein (promoted by the over expression of 38A RNA) in human neuroblastoma cells exerts a deep effect on their excitatory properties, abolishing the fast inactivation of A-type potassium channels. Indeed, in that work, we transfected transiently human cells with a plasmid construct expressing 38A ncRNA detecting the alternative splicing of KCNIP4 and the consequent abolishment of type A voltage-dependent current [
Therefore, in order to reproduce these results in a neuron, we measured the inactivation kinetics of A-type K channels in 38B-transfected primary cultures of mouse hippocampal cells and/or in pMock controls.
The screening of the mouse genome to search for putative pol III type 3 transcriptional units was carried out by means of the algorithm COMPASSS (COMplex Pattern of Sequence Search Software) [
Data on genes number for each chromosome and data used in attended calculation in Chi-square test (
Gene annotations were obtained through the Database for Annotation, Visualization, and Integrated Discovery (DAVID) [
In order to clone 38B transcriptional unit into pEGFP-N1 vector, the insert containing 38B (composed by the type 3 TATA/PSE-dependent promoter and the transcribed portion of 38B) was obtained amplifying 1442 bp of the murine genome with Forward oligo 5′-AGTCATTAATCCTAGATATCAAAAGGATGG-3′ and as reverse oligo 5′-AGTCATTAATTACAGATTTAGCATTATACTAA-3′. The insert was then digested with
Hippocampal neurons were isolated from four seven-day-old mice C57/BL6. Hippocampi were removed, placed in 4 mL HBSS (bicarbonate-free Hank's balanced salt solution, Invitrogen, cat.no.14185-052, Carlsbad, CA, USA) and 0.5 mL of 2.5% trypsin (Invitrogen, cat.no.15090-046, Carlsbad, CA, USA) and incubated for 15 min in a water bath at 37 °C. Next, trypsin solution was removed and the hippocampi washed gently two times for 5 min at room temperature. Hippocampi were dissociated by repeated pipetting in a Pasteur pipette and put in culture immediately onto 20 μg/mL polylysine-treated glass coverslip (for patch clamp experiments) and/or in a pretreated six-well plate (for RNA extraction). Neurons were plated in MEM, supplemented with glucose (0.6%) and containing 10% horse serum; after 1 day, once the cells were attached, the coverslip was transferred to a new plate with maintenance medium Neurobasal/B27, prepared according to the manufacturer's instructions (supplementing Neurobasal medium (Invitrogen, cat.no.21103-049, Carlsbad, CA, USA) with
Total RNAs from samples (hippocampal neurons and mouse tissues (obtained by adult male C57BL/6 mice)) were extracted using TRIzol® reagent (Invitrogen, Carlsbad, CA, USA) as described elsewhere [
Total membrane currents were measured in the whole-cell configuration of the patch-clamp method as previously described [
The external solution was applied to the cell bath by gravity flow perfusion. The potassium currents were elicited from a holding potential of −100 mV by applying voltage pulses 200 ms long from −50 mV to +50 mV in 10 mV increments. The depolarizing voltage pulses of 200 ms duration was applied every 5 s. A best-fit exponential function was used to determine the time constant of the current inactivation.
Traces were analyzed by pClamp10 software (Clampfit) or by ANA, software developed by Michael Pusch, as previously described [
One of the most relevant discoveries of the postgenomic era is that the mammalian genomes are almost entirely transcribed producing a panoply of different classes of noncoding RNAs with regulatory features [
A further relevant aspect of our work is the isolation and functional characterization of 38B in hippocampal neurons. Indeed, these experiments demonstrated that the pol III-dependent regulation of alternative splicing occurring in human KCNIP4 gene takes also place in mice, supporting the use of our promoter-based approach to identify mouse functional homologs of human type 3 transcripts also in the lack of sequence homology between human and mouse ncRNAs. This lack of homology, in spite of functional similarity, might be related to the intronic location of the pol III transcriptional units, and is in agreement with the recent suggestion that extended regions of human and mouse introns are involved in conserved functional links that do not depend on underlying sequence conservation [
Therefore, the experiments reported here show that our promoter-based approach is suitable for the identification of mouse functional homologs of human pol III-transcripts allowing the generation of mouse experimental models for the study of pol III elements.
In conclusion, besides the above mentioned functional studies, the mouse collection of pol III transcriptional units here provided is a valuable source for (1) the identification of novel small RNAs that affect splicing regulation and, (2) in light of the increasing evidence supporting the cell type/stage-specific regulation of pol III transcription, as a novel tool for gene-silencing presidium in therapy and research.
A.P. was supported by MIUR (2007 PRIN Program prot. 2007945BZN), by the Associazione Italiana Ricerca sul Cancro (2009 AIRC Program n° IG9378) and by the Associazione Italiana per la Lotta al Neuroblastoma (Genoa, Italy). G.D. was supported by Fondazione Cariparma (2010 grant program) and by the Italian Ministry of Education, University and Research (MIUR, PRIN Program).
The authors declare no conflict of interest.
38B ncRNA expression in different mouse tissues. Results were normalized to the heart sample.
Hippocampal neurons after 6 (
Expression of 38B eliminates fast inactivation of outward K+ current. Traces were obtained from a control neuron (
The most recurrent functional annotation genes hosting pol III transcriptional units. The table represent a schematic view of the output obtained by DAVID analysis. "Category" indicates the original database or resource where the terms orient; "Term" column lists the enriched terms associated with the gene list; "Count" and "%" are the number/percentage of the genes enriched in a term; "
|List Total
|Pop Hits
|Pop Total
|alternative splicing
|4.64 × 10−29
|1.48 × 10−26
|1.48 × 10−26
|splice variant
|1.46 × 10−24
|2.37 × 10−21
|2.37 × 10−21
|1.44 × 10−17
|2.28 × 10−15
|2.28 × 10−15
|domain: EGF-like 1
|8.94 × 10−12
|1.45 × 10−8
|7.23 × 10−9
|3.63 × 10−10
|1.15 × 10−7
|5.77 × 10−8
|domain: EGF-like 2
|3.65 × 10−10
|5.89 × 10−7
|1.96 × 10−7
|GO:0007155 ~cell adhesion
|5.00 × 10−10
|8.32 × 10−7
|8.32 × 10−7
|GO:0022610 ~biological adhesion
|5.30 × 10−10
|8.83 × 10−7
|4.41 × 10−7
|egf-like domain
|7.54 × 10−10
|2.40 × 10−7
|7.99 × 10−8
|1.33 × 10−9
|2.10 × 10−7
|compositionally biased region: Ser-rich
|5.78 × 10−9
|9.35 × 10−6
|2.34 × 10−6


text:
Over time we will plan on posting positive and/or humorous statements here that come from our customers.
"This place is really going to be awesome - I can't wait to visit and spend every waking moment here!"
Bryan McGonigal
We've not bothered to develop this page... yet.
© 2009-2014 McGonigal's Pub All Rights Reserved.


text:
This practical series examines proven strategies and tactics from an equipment standpoint, one system at a time.
This practical series examines proven strategies and tactics from an equipment standpoint, one system at a time.
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Developed and patented in the Netherlands by Mr. Jan P. de Baat Doelman, Scalewatcher technology was introduced to the European market in the 1980s. With immediate market success, Mr. Doelman brought the technology to the United States and applied for and received a patent in 1991. From that moment, Scalewatcher North America has been on the forefront of environmentally sensitive water treatment. Located in Oxford, Pennsylvania, Scalewatcher North America continues to lead the industry in descaling products that do no harm to the environment.
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text:
So it is discouraging that conservatives may not even want to take the first step down that path.
What followed was a very strange and discouraging series of events.
Given this discouraging situation, Obama took office hoping, as Vice President Joe Biden put it, to "push the reset button."
It seemed to me a miracle that I never heard a discouraging word from my editor, Susan Murcko.
Critics expressed concern that American security was showing an unfriendly face to the world, discouraging travel and tourism.
discouraging as was the outcome of this movement in Ajaccio, similar agitations elsewhere were more successful.
Every day it was a different rule, which in itself was discouraging.
With this answer the messengers returned, and their report was discouraging to the hopes of the exiles.
This sight, instead of discouraging Frank, made him assume an air of bravado.
But such sums came at uncertain intervals, and her board bills came due with discouraging regularity.


text:
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Plumbing Questions? Ask a Plumber and Get Answers ASAP.
Hello and thank you for using JA.com. I will assist you today with your question. Please accept the answer upon completion of the Q&A process.
Hi, and thanks.
it is very difficult to tell which thermostat is faulty, there is no great way to tell which one is sticking on without metering for an entire cycle and catching it in the act of a fault.
hello and your welcome~!
I typically recommend changing both upper and lower at the same time
the cost in minimal, and it is a very easy replacement
That is what I thought-why not avoid this again in the near future.
I recommend always changing them wire for wire as to not confuse which goes where.
agreed. I never just change one. for $13-17 each it is a cheap fix!
Is there any need in replacing the heating elements at the same time?
not unless you have a lack of hot water....by the sounds, on occasion you have too much! haha. you can replace them if you wish while you are at it, but I do not feel it is necessary
That's what I needed to know. You have been a great help, thanks.
my one recommendation....while the power is off, it is never a bad preventative maintenance method to drain and flush the tank
I had planned on that. Thanks
but remember to never turn the power back on until the water has been run through the faucets and all the air is removed!
Thanks for the reminder.
Your very welcome, good luck with the repair, if you need any further information I am typically always available, feel free to come back and ask any other questions you may have.
Thanks Marc.
have a great day and remember to please click on the accept button when you are completed!