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Leslie West and Felix Pappalardi formed Mountain in 1969. Their fourth ever show was at Woodstock, where they went on after the Grateful Dead and scared the sonic bejesus out of some unsuspecting hippies. The group gets its name from lead singer West's onetime imposing physique, which one British publication referred to as "girthsome."
West quickly made an impact as a clever songwriter, powerful vocalist and guitar monster. With drummer Corky Laing, Mountain became a power trio in the style of Cream, and it was Cream's bassist Jack Bruce who wrote "Theme from an Imaginary Western," which remains an all time Mountain favorite. The group's signature song, however, is "Mississippi Queen," which has been heard on fine rock stations and beer commercials since 1970.
To our great benefit, West is not just a formidable guitarist, but also a great storyteller. Here, he shares with us his memories of recording with The Who (and the case of the wrong Felix), being sampled on hit rap songs, and that famous cowbell. West's role in rock music history is, indeed, mountainous.
West quickly made an impact as a clever songwriter, powerful vocalist and guitar monster. With drummer Corky Laing, Mountain became a power trio in the style of Cream, and it was Cream's bassist Jack Bruce who wrote "Theme from an Imaginary Western," which remains an all time Mountain favorite. The group's signature song, however, is "Mississippi Queen," which has been heard on fine rock stations and beer commercials since 1970.
To our great benefit, West is not just a formidable guitarist, but also a great storyteller. Here, he shares with us his memories of recording with The Who (and the case of the wrong Felix), being sampled on hit rap songs, and that famous cowbell. West's role in rock music history is, indeed, mountainous.
Dan MacIntosh (Songfacts): Let's talk about some of your more popular songs. Of course, "Mississippi Queen," I imagine that's your signature song. Do you still enjoy playing that song, and what does it mean to you now?
Leslie: I'll tell you what's really funny. I don't know if you know this, but I had my leg amputated.
Songfacts: Yeah, I heard about that.
Leslie: And it was in Mississippi. I played at the Hard Rock in Mississippi, the Hard Rock Casino. Thank fucking God they had a great surgeon down there. But when I play "Mississippi Queen" now, I think about Jesus Christ. Of all places to lose my leg, it was Mississippi. But I so love the song because I can name 400,000 groups who would like to have a song like that.
Songfacts: Absolutely. I'm sure of it. If you're going to have a signature song, it's great to have one that's that good and not some stupid novelty song, right?
Leslie: Absolutely. The riff is great, the cowbell in the beginning was just in there because Felix wanted Corky to count the song off. So we used the cowbell to count it off - it wasn't put in there on purpose. And it became the quintessential cowbell song.
But actually, when we go to Europe, it's a whole different ball game. "Nantucket Sleighride" is the big draw. In England, there was a TV show called Weekend World, it was like the UK's version of 60 Minutes. It was a 4-hour news show on Sunday, and "Nantucket Sleighride" was the theme song for it. Every time they went to commercial they played "Nantucket Sleighride." When I first went over to England, I found out they still use the pound, they don't use euros and all that. I told my manager, "I need some money." He said let's just go the accountant and get a check." I said, "What do you mean, get a check?" He says, "You have a lot of royalties sitting here." I said, "Why?" He said, "Because we submitted 'Nantucket Sleighride' for this TV show." I was just thrilled.
Songfacts: You never know where your music's going to go and who it's going to impact, huh?
Leslie: You have no idea, man, because, aside from that, I'm sitting in my office right now and I have two platinum records and a gold record on my wall. In a million years I never would have guessed that these people would do my song. You know Jay-Z the rapper? You've heard of Jay-Z?
Songfacts: Yeah, absolutely.
Leslie: "99 Problems" is my song. They sampled that, and Kanye West sampled the same song. The song was actually called "Long Red." It was on my first album. Kanye wrote two songs with "Long Red" as a sample ("The Glory" and "Barry Bonds"). Then Common, another rap artist, used "Long Red" for his intro. So it's absolutely amazing that I got platinum and gold records on my wall. I'm really happy because you'd never figure a Jewish kid writing the top rap songs in the world, so you never know who's going to end up doing your songs.
Songfacts: You're a hip-hop star and you never intended to be.
Leslie: Well, you know what? It's great. Because I always thought to myself, why don't they use heavy guitar in rap? Rick Rubin produced that album, the Black Album, Jay Z's album that "99 Problems" is on. And I think it may have been his idea, because he's a big fan of Howard Stern and I'm always on the Howard Stern Show. Jay Z was going to retire, and then Rick Rubin talked him into doing one more, and the Black Album became the biggest selling album of his whole career. You gotta be lucky besides being talented, if you know what I'm saying.
In March, 1971, Leslie played on sessions with The Who at The Record Plant studio in New York. Leslie's guitar can be heard on the song "Baby Don't You Do It," which was released as a bonus track on the 1995 reissue of Who's Next. You can also hear Leslie on outtakes of "Behind Blue Eyes" and "Won't Get Fooled Again."
Songfacts: I was reading that you were on those sessions with the Who when they were recording Who's Next. Do you have the story from those days?
Leslie with Slash. Photo: Alex SolcaLeslie: I got some story going with that. First of all, The Who's company, Track Records, was our agent in England. They represented us over there. So I got a call from their manager, who's long deceased now, Kit Lambert. He asked if I would like to play on this album - The Who were doing a new album tentatively titled Who's Next. I said, "Why would Pete Townsend need me?" He says, "Because he doesn't want to overdub." He wanted to do straightaway playing with me. So sure enough, we go down there to the studio. I bring my amps, I'm playing with them, going over all these songs and he had demos he did. First time anybody had used one of those synthesizers for "Won't Get Fooled Again." The thing was like one of those things that you use to plug in a phone like at the phone company. You know, they're plugging in all the lines. I don't know if you ever watched the show Laugh In. It might have been before your time.
Songfacts: I've watched some of Laugh In, yes.
Leslie: Well, Ernestine was a telephone operator. She had this board in front of her where she'd be talking. That's what that synthesizer looked like Townsend was using, it was so old. So we're recording, and then Kit Lambert says to me, "Listen, does Felix play the organ?" Felix Papallardi was the bass player in Mountain. I said, "Yeah, I think so. Why?" He said, "Well, I'll have him come down and play a song with you." So Felix comes down and now he's thinking - he had a massive ego, Felix. He's thinking that The Who want him to play bass. So he sends his bass down, and I said to him, "Felix, I don't think they want you to play bass." And John Entwistle walks in the studio and sees all these basses and says, "What the fuck is this? I thought I was the bass player. Now what is this?" And Felix says, "Well, make up your mind, I'm a busy man." So Townsend turns around and says, "Listen, we thought you were Felix Cavaliere from the Rascals that played organ." So there I am, a little fucking fat kid from Queens, and this is so embarrassing. And then he leaves, and there I am with The Who. They had to go back to England to re-record it with Glyn Johns because some stuff didn't come out good. But I know they released it on The Who's remixes years later. Playing with The Who - unbelievable.
Songfacts: That's a great story. Oh to be a fly on that wall.
Leslie: Listen to what's even more funny. At the session there was a big Hammond organ, and I saw Pete sit down at the organ. He was slamming the thing down like he was Phantom of the Opera. He was having a good old time, but he was being deadly serious. This is what a nut job he was. But being able to do sessions with them and have them come out later on was just a great thrill.
In 2011, West released The Unusual Suspects, the suspects being his guest musicians Billy Gibbons, Slash, Zakk Wylde, Joe Bonamassa, Steve Lukather and Kenny Aronoff (the lone drummer). West is out to prove that a 65-year-old Woodstock veteran can still rock with the best, saying, "My singing has gotten better and, as a player, I'm more melodic and have a better vibrato. As I get older, I think I've gotten better."
Songfacts: The new album has really got some great songs on it. And the one that really stands out to me is the one "Don't Call Me a Legend."
Leslie: It's very interesting that you mention that, because the song was written 30-some odd years ago by my partner, Joe Pizza, spelled P-I-Z-Z-A. I went to school with him and we wrote a couple more songs on the album. My wife heard the song, said, "You've got to listen to this song." And originally, "just call me legend" was the first line. I said, "Joe, there's no way I'm going to sing a song that says 'just call me legend.'" So I thought about it and said, "I can sing it if it said, "Don't call me legend, I just came here to play."
He wrote it on piano. The song itself was so beautiful and I love playing lead guitar over piano changes. It's much different than when I'm playing over a guitar-written song. So that just came out fantastic. I didn't think it was going to come out that good, but by God, it did. The words are pretty much true, and the fact is that I've known Joe for 35 years, he was a couple of grades younger than me. Still something seemed to click with that song. I'm pleased that you like it.
Songfacts: What it makes me think is as soon as somebody starts calling you a legend, it's almost as if your best days are behind you.
Leslie with Billy Gibbons. Photo: Alex SolcaLeslie: Yeah, it's like when somebody puts out a retrospective album, that means you have one foot in the grave. (laughs) But the thing is, when I'm singing about the old players, dead or alive, it feels like it's somebody else's point of view, because he wrote the song. I didn't write the song. I don't know if a listener will go that deep in it, but there's a line in there he has about "my fans have changed over time, they come back to see my pantomime." I said, "Joe, what do you mean by that?" He said, "Well, when you play, I notice you play with your mouth." In other words, I'm mimicking what I do on the guitar with my mouth. I said, "Bullshit. You picked that up?" I think it was just great.
Songfacts: Why did you wait so long to record it?
Leslie: I never heard it until I was getting ready for the pre-production for this album. Joe and his partner Ronnie own one of the biggest pharmaceutical companies in the United States (Interchem). I went to school with this guy. I had no idea he was as rich as he is. He's like a normal, simple guy. But my wife now works with him. Every pharmaceutical company on the market has to license the compounds through Joe and Ronnie's company. I'm talking about Pfizer, Smith Kline, all that - they license all these compounds that are going in this country. So they had a recording studio in their offices to make DVDs and CDs for these companies explaining how the compound works. In doing so, I guess about 20 years ago, he turned the studio into a real rock studio, not just to make explanatory DVDs. So I was going up there and doing a pre-production, and my wife saw the song and said, "Listen, Joe wrote this song for you 30-some odd years ago. You've got to listen to it." So I listened to it and I said, "Boy, this would be great for the album if I can do it good."
Songfacts: How crazy is that?
Leslie: Very crazy. Like "One More Drink For the Road," the opening cut, he came in with that, and then I added stuff to it, and we finished writing it together. Their pharmaceutical company, you can't work there unless you love music. Because he's got the recording studio, Joe's got a grand piano in his office, and his partner Ronnie plays drums - it's amazing. It's abso-fucking-lutely amazing.
He's got a lot of spare time and he's able to write these songs. I listened to some of them and he said, "Leslie, if you get an idea, get in the F'n studio and put it down and we'll see if we can write it." So it's great having a full-blown recording studio five minutes from my house that I can go in all the time. And he has other great songs, and he's writing a Broadway play now. He's a really, really talented guy and he's never gotten any credit. His solo career, I think he put out an album called Slice - because he's Pizza. It never really did anything. But I played on that first album God knows how many years ago. He's written some really beautiful songs. That "Legend" song just really gets to me, man.
Songfacts: Well, I wanted to talk about "Theme From an Imaginary Western," and I was reading something that it was dedicated to Felix...
Leslie: Wait a minute, "Theme From an Imaginary Western" dedicated to Felix? Where did you read that? On the original album? (we saw Leslie make the dedication here) You know what? Jack Bruce wrote that song when he was in Cream. I think when we recorded it on the album Climbing!, on the back, I think maybe Felix dedicated it to his mother. It wasn't written about Felix, but basically the song, according to Jack, was about Cream going on the road.
Songfacts: Okay.
Leslie: "When the wagons leave the city"... when we started Mountain, Felix produced Jack's album Songs for a Tailor and he had a really great song on that. So I saw it - took me a while to learn. It was not the simplest of songs. But I love that song. When we play it now I still get excited to play the solo - the chord changes help make that song so great. Very different rock song.
Songfacts: So that's wonderful that it still kind of gives you that feeling, because it's a timeless song.
Leslie: It does. And even with "Nantucket Sleighride," I used to hate playing it because it was so complicated. Now I love it. It sounds like a really great, different type of song for a rock group to do. That's what "Legend" reminded me of; when I'm playing the chords and playing the solo on "Legend." Someone wrote me yesterday and said, 'I thought the album was fantastic, and he said, "You know, this sounds like the newest version of a song in the vein of "Theme From an Imaginary Western."
Songfacts: That's quite a compliment.
Leslie: Yeah, it was really great. Really great.
Songfacts: I want to ask you one last question and I'll let you get back to your busy life. I read that you went to Forest Hills High, which is where the Ramones and Burt Bacharach and Paul Simon went - a lot of really, really famous people.
Leslie: I never heard Burt Bacharach. I knew Paul Simon did. And the Ramones, they were younger than me, but Johnny Ramone lived across the street from me when I lived in Forest Hills. Before they were a group, he wanted to be our roadie. I didn't graduate, by the way.
Songfacts: So you knew Johnny before he was a Ramone?
Leslie: Oh yeah. We used to call him Johnny Beatle because he had a Beatle haircut. He'd walk around, we'd call him Johnny Beatle.
Songfacts: That's priceless. Thank you for taking time and sharing some of those great stories.
Leslie: Thank you. I appreciate it more than you know.
We spoke with Leslie on August 16, 2011.
Comments: 13
Didn't Johnny Ventura the lead singer of the Cat's Meow co-write the song " Long Red " with you?Lou Mast from Cedarhurst . Ny
Weren't you and Felix together in the Vagrants in '67? Ok, I'm at a concert at the "Island Garden" in west hempstead around 1966. The first act was The Illusion, the second act the Vanilla Fudge, the third act was you with Corky Lang, Felix Papalardi. Who was the drummer? Oh, and the next act was of course the Young Rascals. But when you did the riff to the intro Mississippi Queen the f--king place went crazy!! You were signed to Atco Records and years later I was signed to Atlantic 93'. Anyway, during the late 80's we were introduced at a place called Justin's in Cedarhurst, Long Island by a mutual friend, Ronnie. One day if at all possible I would love to sing with you on "Imaginary Western" since people over the years said I have a voice timbre like Jack Bruce. Can I sing? Ask Ronnie.Lou Mast from Cedarhurst . Ny
In April of 1971 I saw Mountain at Scranton's Watress Armory .. At The end of their set ... My friend and I ran around to the back of the stage ... Leslie came down the steps off the stage and went to hand Me His Les Paul Junior ... Then Laughed and Said .. "Hey .. You're Not the Guy" and handed it to a Roadie ... I almost fell over! ... LOLDave from Scranton, Pa.
Don't forget West, Bruce and Laing....whhhhyyyyyyy dont'cha come to my place! Best Corky song ever...Turn Me Over.....Agree that LW is the most underrated rock guitarist in history.......Sanford from Prescott, Az
Interesting story Corky Laing told about Mississippi Queen: the first person outside the band to hear the song was none other than Jimi Hendrix, who was at the Record Plant, mixing Band Of Gypsys, when Mountain recorded it...Jon-a from Madison, Wi
Always loved Leslie West. A sound that can't be duplicated. Still, in my opinion the most unrecognized guitar player there is. He makes it sing. I still get goose bumps when he play the solo to "Theme" to this day.Bob Annese from Originally From Scranton, Pa.
Ramalamaman from Phnom Penh! Wow, thank you for checking in.Dan from Norwalk, Ca
Corky Laing wrote the lyrics to Mississippi Queen; it's about a dancer he dated.Ramalamaman from Phnom Penh
Ya! I saw Leslie West in concert on stage in Passaic, NJ in 1973 or 74. I was three seats away from front row, with a real good view of him on stage. He accidentally droped his electric guitar during the performance, and was really upset (tee'd). He picked up the quitar and was checking it over for damage, during the show. He was mad about it! I guess that "guitar" really meant something to him. Other wise, of course it was a GREAT performance. And this little incident has still stuck with me, till this day. UH! Thanks for that memory LESLIE WEST! Actually it does mean something to me!!!Dan from Anaheim
For more road stories, read Leslie and Corky's book.Steve-o from Los Angeles
Leslie West, YOU DA MAN!!! Wish I coulda asked: What's with the extreme background hiss on MISSISSIPPI QUEEN? Was it a cheap studio/bad pressing/maxed gain during process? I can't say how many times I cursed that noisy recording when making tapes in my younger days, bought several alternate copies and LP's but they all sounded just as horrible… Funny thing is, now it's grown on me and I wouldn't want a clean issue now -- I actually think the hiss brings out a little something extra in that sizzling track. That song when released delivered more SOLID ROCK IMPACT to the soul than anything around: true to its namesake's powerful mesmerizing paddlewheel, it hypnotizes and entrances the listener - who, like the Mighty Mississippi, can neither resist nor escape the encroachment of this relentlessly driving composition, we get sucked in, rolled around and pounded, and spit out abruptly in its wake -- and do we not feel so thoroughly refreshed and invigorated by the experience? (Note to newbie Mountain fans: NEVER IN MY LIFE is in the same vein, a must hear and worth tracking down if you ain't never heard it - it's nearly MISSISSIPPI QUEEN'S equal in hit quality with even more soul (if you can imagine that!), and its debauched imagery of his awakening "in the mornin" to a good woman that brings whisky & lovin to her man for breakfast was doubtless too risque for airplay til decades later. Yes - there were other great, powerful, more famous, larger sellers, but MISSISSIPPI QUEEN muscles its way to the top o the heap in my book for its unique combination of bare-bone arrangement, driving/pounding tempo, ragged power guitar, no bullsh!t power drumming, high-impact vocal; of course the contenders have all these requisites, but none match the sheer hair-raising ELECTRICITY exuding from this gnarly slice of Americana, nor do they exhibit the loose spontaneous feel of this most precious session!! Leslie's Real Man guttural vocals and imposing delivery make Daltry and Plant sound nearly cherubic by comparison, no disrespect intended but I prefer a male singer who sounds more like a man. Also- WHERE OR WHEN WILL WE GET MORE JUICY DETAILS OF THE WHO'S NEXT SESSIONS? Do the Who fans know this? Upon initial consideration, it makes plain sense that Our Man West would be the singular choice for augmenting Squire Townshend during this Who's Next zenith of his ingenuity. Dang it: now I'm going crazy trying to suss who's playing what! Any hints for us, Mr. West??? Godspeed, Blessings, and THANKS to you, Sir! Leslie West is absolutely one of the unsung greats, how enlightening it is to find he is also a great man. Great article/more please! -Mark the SharkMark The Shark from Mile High City Denver Colorado
My friend's mother works for Interchem! Joe Pizza's Her boss! I totally freaked when I read that!Nick from Paramus, Nj
Nice interview. Sorry to hear about his leg, glad he is OK though.Jim from North Billerica, Masee more comments


text: Tweet « Back Header Content Beta Test and Beverages Try out a new new and innovative online professional exchange called bridgemycareer, and afterward we'll head to Jake Melnick's. The first round and appetizers are on Alumni Relations! Loyola is pleased to offer our young alums and current students access to bridgemycareer. There is no charge to the grad/student for the service. bridgemycareer's proprietary software collects abundant information including your credentials, capabilities, experiences, interpersonal skills, and personal preferences. It then aligns this information to similar job-specific criteria set by leading employers, using a flexible and powerful algorithm. So, instead of you trying to find a job... the jobs will find you! Jobs that are right for you whether you're seeking permanent, project-based, or internship opportunities. Online Registration for this event has closed. Walk-ins will be welcomed at the door. Register Here Contact InformationAlumni Relations820 N. MichiganSuite 911Chicago, Illinois email@example.com & LocationDate: 3/7/2013Time: 6:00 PM to 7:30 PMLocation: Lewis Towers, Water Tower Campus111 E. Pearson Computer Lab 410 Chicago, IL 60611

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Just hours earlier, at a Las Vegas court, she had admitted possessing cocaine and obstructing a cop. She was given a 12-month suspended jail term. "Paris was contractually bound to her business trip and didn't want to let down her brands and many Asian fans," the Sun quoted her spokeswoman as saying. "She is trying hard to do the responsible thing, but this is beyond her control. She is very disappointed," she added.
Paris - ordered to attend drug counselling over her cocaine rap - flew into Tokyo by private jet. Her sister Nicky, 26, and pals were waved through, but Paris was detained. Japan allows in those serving suspended jail terms only for 'special reasons'. The heiress and Nicky had been due to promote Paris's new fashion line in Tokyo today but the event was cancelled.


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2010 IHF Super Globe all set for spectacular start
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Today Monday, the 2010 IHF Super Globe in Qatar is all set for a spectacular opening as the participating handball champions from across the globe descend upon Doha with some of the top players in the sport in their ranks.
"We are ready to receive the handball champions from each of six continents here in Qatar, a country with an outstanding passion and potential for handball. The organising committee has completed final venue checks and believes the visiting world of handball will be highly impressed with what they see in Doha. We wish all the participating players – including some of the very best players in the sport of handball – the best of luck in the competition. With such an array of top teams and star players on offer, we are certain Qatar will witness a handball festival which will thrill our expectant spectators as well as travelling fans," said Mr. Saadoun Al-Kuwari, Event Director, IHF Super Globe Organising Committee.
A fascinating Group A encounter indeed awaits fans on Monday to open the tournament, as defending IHF Super Globe Champions BM Ciudad Real take on African champions Al Zamalek at 5:45 pm at the Al Sadd Sports Club venue. The Egyptian team will be buoyed by its many Middle Eastern fans, but BM Ciudad Real comes fresh from claiming a fourth consecutive Spanish League crown and will be looking to add more silverware to a sparkling trophy cabinet here in Doha. Qatar's numerous handball fans can certainly expect a thrilling opening game, and won't be thinking about leaving the venue thereafter either as home team Al Sadd of Qatar take on their namesakes Al Sadd of Lebanon at 7:15 pm. The Group B encounter will be a passionate affair with both teams looking to get their tournament off to a winning start, and it will also be a Middle Eastern derby which reflects the growing status of handball in the region.
"We are extremely proud to host such a prestigious and important tournament in Qatar for the next four years, and we believe that it will give a further boost to the sport of handball in the Middle East and really propel the game to another level. This tournament will give our youngsters the best indication that they too can make it onto the world's biggest stage," Al-Kuwari added.
Group A is completed by Brazilian champions Unopar, who spring into action on the second match day on Tuesday as they face BM Ciudad Real at 5:45 pm. In Group B meanwhile Australian side Southern Stars will be attempting to book their place in the semi-finals when they start into the tournament on the second day. Southern Stars will take on Al Sadd of Qatar at 7:15 pm on Tuesday, before the final round of group matches begins on Wednesday. The semi-finals take place on May 20, before the grand final of the IHF Super Globe on Friday May 21 will decide the 2010 champions. Expectant excitement is certainly in the air in Doha already, just before the tournament begins.


text: Dj Raid was borned in 1989 in Tarragona (Spain) having his first contact with music productions at 12 years old, after buying a simple program wich showed him the basic tecnics of music productions. He started composing gabber/holand hardcore tracks. At the age of 14, he starts to work at the local radio doing his own programs during 3 years. In the same period, he´s into djing in local clubs. After passing through Monegros Festival and living acts like Pet Duo or Dj Rush, he starts getting into techno wich will drive him to the work of people like Richie Hawtin,Marco Carola, Ricardo Villal..DJ Raid's biography DJ Raid Musician


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Beauty mole
Monday 19 September 2011
Antipodium Presentation by M.A.C Senior Artist, Caroline Donnelly
"We arrived nice and early to the Portico Rooms at Somerset House. When we got into the hair and make up room, it was already a hub of activity with the hair team and models already milling about. This was a good start to the day as everyone was ready to go even before call time. Georgina Graham designed the look for today's Antipodium presentation. The casting of the show included male and female models and ranged from a 16 year old school girl with braces, to an elderly 83 year old lady who has the most beautiful long silver hair. The look was cross generational with an element of androgeny, which suited both male and female, young and old.
We applied minimum base using MAC face and body foundation, a little concealer where needed and well placed MAC prep and prime pressed power near the nostrils to keep the skin glowing but to prevent it looking oily. Brows were brushed upwards and made to look more 'boyish' using MAC brow pencils. The brows were finished using a mascara like brush dipped in MAC Pro Mixing Medium Shine. This gave the brows a healthy and glossy look. It also complimented the hair, which was held in place using a shiny wax.
The looks were finished off with a healthy glow using MAC Cream Colour Base in Bamboo on both the cheeks and the lips.
Colour was added using fingertips to the lips so that the effect was not like lipstick, but more melted and pushed into the skin.
The blush was quite horizontal on the cheeks and applied also with fingertips, which made it look like it had an affinity with the skin. The make up was finished without too much stress and models were dressed and lined up backstage, ready for the runway.
It was just down to us to check the bodies for bruises and cover them up with concealer, check for shiny noses. The look was minimal but the use of a light covering of base, teamed with the glossy brow and the peachy cream colour base in Bamboo, gave an elegant, yet sporty look."


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Hello! Thank you for taking time to view my profile. I am looking for someone to date and spend quality time. I would eventually like to be in a long term relationship.
I am easy going and down to earth. I am caring, compassionate, thoughtful and generous; I am sensitive yet strong. I am romantic and sensual. I can be stubborn and hard-headed as well. I'm not perfect.
You are open and honest, listen and communicate well, have a sense of humor and know how to be playful. You like to hold hands, cuddle and think kissing is underrated. You aren't perfect either but we would be perfect for each other.
I like walking and hiking. I love amusement parks! I like music in outdoor venues. I love going to a baseball game! I think art fairs and flea markets are fun. I love a good thunderstorm! Other interests include wine tasting, reading, , traveling ,walking, art, and museums.
I enjoy working on jigsaw puzzles and crossword puzzles, playing Scrabble and Words with Friends.
Let's meet, talk, gaze into each other's eyes and see if a spark ignites.
Eating Paleo
Making stained glass.
My smile
Just started reading To Kill A Mockingbird (No, it wasn't required reading for me back in the day)
I haven't been to a movie in a while.....want to go?
I like to watch Criminal Minds, , Blue Bloods, Wayward Pines, Murder in the First, Proof, Scandal, Chicago PD, Chicago Fire
I like all kinds of food, especially Italian, Mexican, BBQ, Chinese and Sushi--which don't always work with Paleo
My friends
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We happen to have some common interests.


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LiftUp! Educational Events: Research
The LAB Miami
Thursday, June 13, 2013 from 6:00 PM to 7:30 PM (EDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
Research is more than a step in the scientific method. Join us as Anthony Mundis, founder of the research firm Impossible Real, explains how market research could make the difference for your business or startup.
Course Overview
- The importance of research when starting or running a business.
- The process of conducting market research then, and now.
- The relevant types of research that will help your startup or business.
- How to get investors interested in your business.
- Understand the difference between standard & startup market research.
- Ways to refine your product, audience, and problem space.
Who's Speaking?
Anthony Mundis graduated with his Masters in Philosophical Psychology from the University of West Georgia in 2011. His background and experience is in social sciences research. He founded Impossible Real in 2012 with the goal of working with the startup community to help them conduct sound market research quickly and affordably. He has forged partnerships with several incubators across the Southeast (GigTank, CoLab, and ProjectLift Miami 2013), helping companies get to the next step in their business.
Orginized by Project Lift Miami 2013
ProjectLift Miami2013 is Miami's first and only seed accelerator focused exclusively on the intersection of health and technology. Our mission is to catalyze and support innovation that facilitates quality healthcare services, better patient health, and better healthcare outcomes at a lower cost.
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400 Northwest 26th Street
Thursday, June 13, 2013 from 6:00 PM to 7:30 PM (EDT)
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Trial of mom accused of killing kids continues
TAMPA, Fla. (AP) - The ex-husband of a woman accused of killing her two teenagers is on the witness stand in her Tampa trial.
Defense attorneys on Tuesday called Parker Schenecker as a witness.
He and Julie Schenecker were married in January of 2011, when police say she shot and killed their 16-year-old daughter Calyx and their 13-year-old son Beau in the family's home.
The couple divorced after the deaths.
Parker Schenecker told the jury Tuesday that his wife's depression and mental illness was a constant "drum beat" in their relationship.
Schenecker is charged with two counts of first-degree murder. The state is not seeking the death penalty and contends Schenecker knew right from wrong when she bought a gun and shot her children.
Photo Credit: Pool (TBO)


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J Bizness Top Five
Heavily influenced by producers like DJ Battlecat, DJ Premier, and J. Dilla, J. Bizness takes a fresh approach to creating music using unconventional sounds while marrying them to the classic West Coast flavor. Using Reason 4 and Recycle 2.1, J. Bizness produced his debut LP, Reason: Beats & Pieces; a masterful work that displays the wide spectrum that is Hip-Hop.
You're Not Leaving (Reason Factory Sound Bank / Subtractor Patches / FX)
This is a common patch used in a lot of my instrumentals. This particular sound serves as either a sweeping ambient effect when the key is sustained, or a bass heavy 808 kick drum when the key is set to a short release. The bass response of the quick attack really rattles the room and I love it! It NEVER gets old to me.
Shore Thing (Reason Factory Sound Bank / Subtractor Patches / FX)
Here is another patch that I use often. When a key is sustained, this patch is a good Foley effect for ocean or beach ambiance. I use it mostly as a rhythm effect in my instrumentals. I usually apply a sharp attack to a key and alternate multiple keys across the keyboard to give an instrumental a very heavy "breathing" effect, similar to that of Darth Vader.
Cork (Reason Factory Sound Bank / Thor Patches / Signature Patches / Tipper (TP))
I have a tendency to use FX to establish rhythm or mood for my instrumentals. This particular patch sounds really interesting when played in short stabs and creates a spacey "Morse code" effect. I usually apply a staccato pattern throughout a large range of the keyboard and alternate the key/pitch to give this patch an effect similar to that of a DJ scratching & cutting a record using a crossfader.
Ageless Exposition (Reason Factory Sound Bank / Combinator Patches / Pads)
I really like this particular patch because the sound is very sweet and subtle. This patch in its preset stage has a fairly quick attack response and makes very pretty chord arrangements. This patch has a ghostly ambiance to its character without adding any Aux effects.
etiK rM [Harmonium] (Abbey Road Keyboards / Instrument Patches / -Style Patches)
All of the instruments in this bundle were sampled and programmed with very realistic detail. When using this patch, the hiss from the mic or amp emulation give it that dirty sound that I like for Hip-Hop instrumentals. You can even hear the scale mechanisms when playing the keys, which makes this patch really cool.
More Charts
Javad's Top Picks
Javad is a keyboardist/MD and producer for various artists including the Timbaland Camp, Warren G, and Jam and Lewis.
Your Infamous Harp Top 5
Your Infamous Harp produce delicately destructive jungle/glitch-hop in the Renaissance tradition.
Josh Head Top Five
Emery is a Post-Hardcore band from Rock Hill, South Carolina currently based in Seattle, Washington.
Harald Boyesen Top Five
Writes and produces scores for Film / TV, and produces bands from Sweden to San Francisco.
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Celebrity Chef, Author, Speaker and Recipe Developer Susan Irby, CFNS, CFH, shares healing, healthy recipes, diet plans and wellness research.
REMEDIES and RECIPES - Dr. Bond and Chef Susan Irby's Healing Classroom
Register Now and you will be able to access the REMEDIES and RECIPES healing e-classroom for a weekly lesson on a health condition, the natural means to reverse it and the tasty healing nutrition-rich recipes to help your body heal.
Comfortable, form fitting, shapely lycra fabric that is versatile to tie and style, the Versakini is a must-have for your bikini swimsuit collection.
Susan Irby, now Certified in Food Healing, shares her story of reaching the bottom and recognizing healing foods were her way up and out… off the bottom.
Drinking fresh squeezed lemon juice is part of the daily plan on The Bikini Chef® Diet and lemon juice and zest are frequently used in The Bikini Chef® recipes.
Most commonly found in powder form, maca root powder has a mildly earthy flavor and can be easily sprinkled onto virtually all dishes such as salads, cooked chicken, side dishes such as Brussels sprouts, spinach, and kale – sprinkle a half to one teaspoon of maca on virtually any food or blend into vegetable and fruit smoothies.
Healing, healthy bikini destinations are those that nurture the mind, body and soul. A beautiful respite within the hectic Las Vegas, Mandarin Oriental is a true bikini lifestyle destination.
Expect the un-expected when you visit PALA Casino Spa and Resort! Situated in the beautiful hills and valley of the Palomar Valley in north San Diego County, this casino spa and resort is like no other.


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In his third novel, Ted Heller has created a character completely unaware of his many social and personal flaws.
Frustrated novelist Frank Dixon (no relation to the author of the Hardy Boys series) has submitted his third novel, "Dead on Arrival" (hint, hint) for publication, but can't get through to his agent, Clint Reno, despite numerous e-mails, at first polite, but soon disintegrating into missives like this:
Haven't heard from you in a while and I hope you are well. I'm not. I've been deathly ill of late. Very bad. Spent 4 nights in the hospital. Fever of 105.5
With that in mind, can you inform me as to the fate of DOA? Anything new on it at all? Bad news, good news?? Anything? It really, really would help me to know right now. I'm in a bad way.
Thank you so much.
To ease the stress of not knowing the fate of his work, Dixon turns to an on-line poker site, where he makes friends, develops schoolboy crushes on women based on their avatars, and amasses a great deal of money. In fact, he makes so much money playing poker online that he gives up his job
Dixon has a loyal wife who goes off to work in New York City every morning, while he loses hours at a time playing cards in cyberspace as Chip Zero.
Dixon's virtual life soon takes over his real one. He continues to win a lot of real money, which allows him and "Wifey" to head to the islands so he can meet "Artsy Painter Girl," his current online amore, who's on vacation there with her investment banker husband and two children. That's where the book goes off the rails.
Although she has plenty of clues (jewelry, a chinchilla coat, exotic vacations), and she's no dummy – she has a high-powered job and makes good money – Wifey can't seem to put two and two together.
Even after discovering Dixon hiding across the street at a Starbucks when he should be at work, Wifey doesn't put the pieces together.
I finished the book only because I wanted to see how Heller snapped Chip Zero back to reality.
No spoilers here – suffice it to say the boy goes down, in a big way.
But does he learn from his mistakes? No.
Heller is a clever writer, but not clever enough to make Frank Dixon worth my time.
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The Burger Company
|The Burger Company
|Associated names:
The Burger Company was Roadchef's attempt at an own-brand fast food outlet. The Burger Company replaced Wimpy which had been at most of their services for over 10 years previously. They used The Burger Company as a holding name for sites which were soon to gain a McDonald's outlet, which was much more popular. This was the first time an operator had ever tried to tackle the fast food market with their own creation.
The Burger Company was trialled in Spring 2010 at Sandbach and Northampton northbound.
The Burger Company was not at all inspired by Burger King. Honestly.
Roadchef announced in September 2012 that they would be slowly fading out The Burger Company in the coming years in favour of McDonald's which they planned to roll out to a majority of their sites. Finally, on 4 January 2016, the final The Burger Company outlet closed at Roadchef's HQ site of Norton Canes.
Former Locations
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- Norton Canes (M6 Toll)
- Rownhams (M27)
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- Watford Gap (M1)
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|Re: [C++-sig] Boost.Python call policies at runtime...
|Stefan Seefeld
|Thu, 09 Nov 2006 11:34:40 -0500
Kevin Jones wrote: > Hi all, > > Given a class called Variant that can (at runtime) > contain either an int or a T*. > > I.E. > class Variant { > public: > int type; > union { > int i; > T* t; > }; > }; What is 'T' ? > How can I create a call policy that uses > return_by_value when Variant is an int or > manage_new_object when Variant is a T* (based on the > value of "type" at runtime)? What do you want to call that returns either of the two ? > Note: I need to wrap either the int as a python object > or T* as a python object and not Variant. You have given far too little information about what you want to do (both in C++ as well as python) for anybody to be able to help. Regards, Stefan -- ...ich hab' noch einen Koffer in Berlin... _______________________________________________ C++-sig mailing list C++-sig@xxxxxxxxxx http://mail.python.org/mailman/listinfo/c++-sig
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On Thursday's episode of The Starters, the guys discuss Steph Curry's 51-point night, whether it was more impressive than Klay's 52, and just how smart the Warriors look for not trading for Kevin Love in the offseason. That, plus the Hawks' team honor, Jacque Vaughn gets canned, the Meme Team, and a new game called, "Guess That Bobblehead." more info: http://goo.gl/xQhEfd
More from


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bang on a can all-stars
bbc singers
bbc symphony orchestra
britten sinfonia
bryce dessner
calder quartet
conor j. o'brien
daniel bjarnason
david lang
glen hansard
joby talbot
julia wolfe
nadia sirota
nico muhly
paola prestini
pekka kuusisto
philip glass
richard reed parry
shara worden
so percussion
the sixteen
trio mediaeval
valgeir sigurdsson
Image: Oceanic Verses
Paola Prestini
is a composer, director and a co-founder of VisionIntoArt, an interdisciplinary production company based in NY, and the creative director of a new Brooklyn based venue, Original Music Workshop.
She has been commissioned and performed by ensembles and organizations such as Bay Chamber Concerts, Brooklyn Academy of Music, Chicago Symphony Orchestra, Concert Artist Guild, the Kennedy Center, Kronos Quartet, Carnegie Hall, New York City Opera, and VisionIntoArt in venues and festivals worldwide.
Her current projects include Aging Magician, recently workshopped at the Prototype Festival with Julian Crouch, Rinde Eckert and the Brooklyn Youth Chorus; the Labyrinth Installation Concertos, a commission at the Krannert Center for soloists Maya Beiser and Cornelius Dufallo and artist Erika Harrsch; the Hubble Cantata, a commission for the Hubble Telescope's 25th anniversary for ICE Ensemble and soprano Jessica Rivera with film by Carmen Kordas; a new work for the Lar Lubovitch Dance Company for Lar's 70th birthday celebration; a new work for Young People's Chorus; and a residency at the Armory in partnership with the Watermill Center, and Beth Morrison Projects.
Paola has received awards from ASCAP, NYSCA and the LMCC and is a Paul and Daisy Soros fellow, and a Sundance Fellow. A graduate of the Juillard School, she studied with Robert Beaser, Samuel Adler, and Sir Peter Maxwell Davies.
See her multimedia opera,
Oceanic Verses
Oceanic Verses by VisionIntoArt


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On a recent November evening, LSU Communication across the Curriculum (CxC) hosted a panel discussion that seems destined to become a regular event on campus. Inspired by the international discussion trend regarding women in leadership, CxC put together a panel of local women business leaders to talk with students about their experiences—from the time when they were undergraduates and interns through their current positions of power. These women gave the attendees tips ranging from what to do and not to do in a job interview to how to keep their jobs and rise to the top. The near-capacity student audience asked questions, listened intently to anecdotes and advice, and later mingled with the panelists.
This panel was a timely discussion given the national popularity of best-selling books like "Lean In: Women, Work, and the Will to Lead" by Sheryl Sandberg and "Pushback: How Smart Women Ask—and Stand Up—for What They Want" by Selena Rezvani. In Sandberg's book, she makes the point that women are far more likely to be affected by negative feedback and criticism than men. Such negative feedback, delivered maliciously, can cause women to question their authority and credentials and can jeopardize their chances to advance. The tips and anecdotes related by the panelists bear out this assertion. In fact, many of the panelists' experiences tracked with these two authors' experiences and the academic studies they cite. Bringing local leaders to talk with LSU students reinforces the importance of recognizing these challenges and finding ways to overcome them.
The panelists, all of whom work in the Baton Rouge area, were Gentry Brann, vice president of global marketing, CB&I; Dima Ghawi, manager of talent development, IBM; Ellen Martin, environmental, health, and safety leader, Dow; Mikki Ceasar Mathews, government banker, J. P. Morgan; and Kathy Victorian, senior marketing representative, Amerigroup Louisiana. Lexi Debrock, marketing coordinator, Postlethwaite & Netterville, moderated the discussion; Debrock is also a 2008 LSU Distinguished Communicator graduate.
These women provided the students in the audience with information that even those of us—both men and women—who had long been out of school wished we had been given while we were undergraduates. They related their own missteps and miscommunications as well as their triumphs. Those anecdotes about communication mishaps were especially enlightening and helpful, letting the audience of juniors and seniors know that even leaders make mistakes from which they learn and grow. These incidents included anecdotes about misunderstanding culture ("only those in the highest positions of power face the door in Japan"), poor listening, project miscommunication, and overlooking typos with significant implications.
The panel tackled a wide range of issues—including emotions in the workplace, appropriate workplace behavior and dress, body language and speech patterns in job interviews, mentorships, confrontations, assertiveness, and social media uses—during the hour-and-fifteen-minute discussion. Students asked questions from the floor and also tweeted their concerns to the CxC Twitter feed, which was monitored live throughout the discussion.
Rebecca Burdette, associate director of CxC, credits both students and potential employers for the topic of this discussion. "We continuously poll students to see what they are interested in learning more about and what skills they feel they need to develop," Burdette explained. "Employers have also encouraged us to address with students effective communication in the workplace. They see this as critical to students' post-graduation success."
Students who attended the event agreed with this assessment. Several cited the panelists' collective recommendation to "be bold."
"The panel did a fantastic job of easing several fears about the professional world and simultaneously inspired me to take pride in my ambitions," Erin Kenna, a senior in mass communication, commented. She was especially affected by Gentry Brann's advice, adding, "She encouraged us to have confidence and authority as women in the workforce."
|Panelists advice and anecdotes
|"If someone becomes angry or upset, use the incident as an opportunity for self-discovery: Ask yourself why the person reacted that way; ask why others are emotional. You may not be able to change the actual 'why,' but you may be able to improve your interactions in other ways."
|"Use the 'yes-no-yes' approach: First, find something good to say about the situation; then state the problem as tactfully as possible; finally, state another positive point."
|"Be open to criticism and feedback. Learn from it."
|"Body language and voice volume are critical in communication. Avoid distracting behaviors, and speak up with authority—even if that's not natural for you or you don't feel it."
|"Think about what you're trying to accomplish, and OWN IT; convey confidence. Don't let yourself be intimidated."
|"Be bold—have courage, hold on tight, but be charming. The balance between boldness and charm is essential."
Kelsey King, a junior in mass communication who also worked with the CxC staff to monitor the Twitter feed and tweet for the event, agreed with Kenna. "I enjoyed all the panelists' advice and really took heart when Gentry Brann said, 'Above all else, be bold,'" she remembered. "All of the panelists emphasized the importance of having confidence."
Sarah Corie, a first-year student majoring in political science, found a bit of Kathy Victorian's advice to be particularly enlightening. "The most important thing the panel discussion taught me is that being an effective communicator is not a characteristic one must be born with," she explained. "Being an effective communicator is accomplished by practicing and realizing your talents, being willing to learn and grow, and, as Ms. Victorian said, 'owning it!"
Corie, who was not yet in the LSU Distinguished Communicator program, made up her mind after attending the event. "I heard about the program earlier in the year and planned to find out more at some point," she recalled. "When I saw on the Feminists in Action Facebook page that the 'How She Does It' event was happening, I decided to begin the process of becoming a Distinguished Communicator."
In addition to educating students about the importance of building strong communication skills regardless of their major, this event was also intended to attract students like Corie to the LSU Distinguished Communicator program which is managed by CxC. While CxC plans to have more events like this one in the future, students who are already in the program know its value.
Tran Tran, a senior in mass communication, enjoyed the panel but is much more focused on the overall benefits she has gained from CxC. "Aside from workshops like this, the program has helped me build a diverse communication skill set and a portfolio of works that I can use in my job search," Tran explained. "This is a worthwhile program for any student."
The successful business leaders who gave of their time to talk with these students would obviously agree.


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Here's a look back on our Top Ten Pi Posts from 2013!
1. 400 GH/s Bitcoin Miner springs to life with Raspberry Pi
2. The GamePi – Raspberry Pi Game Boy case mod #piday #raspberrypi @Raspberry_Pi
3. Solar Powered, Outdoor Raspberry Pi @Raspberry_Pi #piday #raspberrypi
4. Raspberry Pi Status LEDs Explained #piday #raspberrypi @Raspberry_Pi
5. AmbiPi Ambilight Clone #raspberrypi @Raspberry_Pi
6. WebIOPi : control your Pi's GPIO with a browser #piday #raspberrypi @Raspberry_Pi
7. PiMiner Bitcoin mining machine @Raspberry_Pi #raspberrypi
8. 10 More Mind-Blowing, Skill-Building Raspberry Pi Projects #piday #raspberrypi @Raspberry_Pi
9. Cables & Adapters for the Atrix Raspberry Pi Laptop
10. An A to Z Beginners Guide to Installing RetroPie on a Raspberry Pi #piday #raspberrypi @Raspberry_Pi
Read More Raspberry Pi posts here!
Check out all the Circuit Playground Episodes! Our new kid's show and subscribe!
Have an amazing project to share? Join the SHOW-AND-TELL every Wednesday night at 7:30pm ET on Google+ Hangouts.
Join us every Wednesday night at 8pm ET for Ask an Engineer!
Learn resistor values with Mho's Resistance or get the best electronics calculator for engineers "Circuit Playground" – Adafruit's Apps!
Maker Business — HAX Hardware Trends 2017
Wearables — Tidy wires are happy wires
Electronics — Coin Cell Current
Biohacking — Circuit Board Borg Leg Tattoo Sleeve
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As a Volunteer at Capital Repertory Theatre, I agree to the following:
- To work one performance of each production.
- To arrive on time for each of my scheduled performances (one hour before curtain).
- To notify the House Manager at least 48 hours in advance if I am going to miss my scheduled performance and to schedule a replacement. Also reschedule to another performance when needed.
- To arrive alone for my assignments unless I have prior consent from the House Manager.
- To be ready and willing to perform the following duties including (but not limited to): ticket tearing, handing out programs, seating, stuffing programs, guarding the set, and greeting patrons.
- To participate fully in all activities before the show, during intermission, and immediately following the show. This includes (but is not limited to): picking up trash in the house before and after the show, cleaning up spills, assisting patrons in need, enforcing theatre policies (no photography, no food, lids on hot drinks, etc.).
- To wear the Volunteer Uniform (dark bottoms, a white shirt, and a nametag) when reporting for my assignments, so that I may be easily recognized by patrons who may need assistance.
- To be prepared to assist the House Manager in cases of emergency.


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- 34 total posts
- 27 comments
- 7 questions
Questions from Ziongirl
Fat Injections into breasts with Breast Implants - can this be done? Is it safe?
I had my breasts done in Auckland in May this year. I always knew one breast was slightly larger than the other , I have got Teardrop 410CC Silimed Implants and one breast is... READ MORE
I had my breast surgery May 2014, 410cc. Need help choosing implant for my revision surgery! Round or stay tear drop?
I breast fed 2 kids so had very empty breast sacks. I Had 410cc anatomical silimed polyurathane implants. 12.3 width & height, 6.6 projection & 8.3 from bottom implant to... READ MORE
Silimed implants - why are they not used in the US?
Hi docs, here in nZ, surgeons tend to use the same implant range from Silimed. Why are they not used in US? Seems that Mentor or Naturelle are the pick in US. Also NZ often do... READ MORE
Do US plastuc surgeons use German Polytech implants?
I just wondered if You know of and/or use the Polytech implants? Are they a good brand compared to Silimed? READ MORE
Revision Breast implants - Silimed XH round or H round?
I'm having a revision done in 3 weeks and I'm worried about the choice. I have 410cc anatomical implants currently that were put in 1 year ago.. To me they are not big enough &... READ MORE
Can armpit scars from hyperhidrosis surgery easily be revised?
I have my second BA coming up in two weeks time and I was wondering whether it would be worth asking my ps to revise my old scars under my arms from a hyperhydrosis surgery I... READ MORE
Width of chest - help please
Hi have 410cc Anatomical implanta and having my revision in two days. Chest width is 12.5 before first surgery. Options for replacements implants are 560cc H 13.5w or 565cc XH... READ MORE
Recent comments from Ziongirl
Ops, sent by accident! Have more side boob than I imagined.. Will keep u updated READ COMMENT
You have such an amazing body girl! Boons are looking good too, are they starting to fluff yet for you? I haven't done my review cause I need to not be able to name surgeon.. But I'm doing good second time so much easier..,have more... READ COMMENT
Nope not at all.. I guess the measurements were right for me READ COMMENT
Well I had tear drops in before with quite little upper pole, hopefully round will do the job. Well your body frame is smaller than mine so a 500cc would look bigger on you than on me! Significant others are important too :-) READ COMMENT
Hey love, I ended up with 565cc XH (UHP) I did want the 560cc H but they were too wide for my frame. So I went from 410cc to 565cc hope that enough of a jump up to notice more upper pole. Haven't done any stories on here yet I might do... READ COMMENT


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text: New Uses for Office Supplies Facebook Pinterest Twitter Print Mail Save Left Arrow Right Arrow Photo by Monica Buck Left Arrow Right Arrow 30 of 88 Grid View See All Binder Clip as Linens Organizer Store sets of napkins clipped together and they'll always be ready to set the scene at dinnertime. You'll restore order to your linen closet and save minutes searching for elusive matching colors. Start


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Plastic 1/2" Water Valve
You should get this water valve...WATER you waiting for? HAH.
We don't know much about it, other than it's plastic. The openings are approx. 1/2" wide. One end has a bare flange, and the other has a flange with a screw-on thingie. It's a ball valve. Great, right? Only 75 cents.


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Check out this Fast Company story about Studio 360 and Bruce Mau Design's inventive campaign to rebrand Canada.
Author Archives: amandammalek
Image of the Day, February 22, 2013
Finishing up Oscar week, here's another excellent piece of 2013 Oscar art. This infographic, featuring statistics about the best acting and picture nominees, was illustrated and designed by Sam Gilbey for Virgin Media.
Image of the Day, February 21, 2013
Excellent interview of illustrator/designer Tad Carpenter at Grain Edit which includes a look inside his sketchbook and a lot of inspirational work, like the above Bob Dylan poster.
Image of the Day, February 20, 2013
High concept, intricate Bicycle playing card set design from Ginger Monkey's Tom Lane. Read the story and see more photos at The Dieline.
Image of the Day, February 19, 2013
It's Oscar week and the official Oscar website has a gallery of stylishly-redesigned posters for the Best Picture nominees. Above is a reworking of Argo by artist Anthony Petrie.
Image of the Day, February 15, 2013
I Love Typography has a good read about an ongoing project between a letterer (Martina Flor) and a caligrapher (Giuseppe Salerno). While related, the disciplines, styles and techniques of each are very different. Check it out here.
Image of the Day, February 14, 2013
Who doesn't love a good Valentine's Day pun? A collection of cards from Handmade By Machine. Check out the entire set here.
Image of the Day, February 13, 2013
Inventive illustration by Madrid, Spain-based artist Juan Carlos Paz. Via Looks Like Good Design. More here.
Image of the Day, February 12, 2013
Alissa Walker was recently tasked by Studio 360 to redesign Valentine's Day, from the iconic heart symbol down to replacing creepy, cherubic Cupid (the above image is Amanda Woodward's option: the CVO). Read the entire story here (via Under Consideration).
Image of the Day, February 11, 2013
Exclamation Point (Chartreuse) by sculptor/artist Richard Artschwager, who died this weekend. Obit via the NY Times: http://nyti.ms/WZVAYX Photo by Robert McKeever.


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Detention News
Legislation Watch
The National Immigrant Solidarity Network
(ISN) is a coalition of immigrant rights, labor, human rights,
religious, and student activist organizations from across
the country. We work with leading immigrant rights, students
and labor groups. In solidarity with their campaigns, and
organize community immigrant rights education campaigns.
From legislative letter-writing campaigns
to speaker bureaus and educational materials, we organize
critical immigrant-worker campaigns that are moving toward
justice for all immigrants!
National Immigrant Solidarity Network
No Immigrant Bashing! Support Immigrant Rights!
mail: email@example.com
New York: (212)330-8172
Los Angeles: (213)403-0131
Washington D.C.: (202)595-8990
to Arabic | Translation
to most European Languages
6/8: Trumps deportations of Dreamers could usher in new economic, legal woes
By Ambar Pardilla - POLITICO
President Donald Trump has made conflicting comments about the status of undocumented immigrants who entered the country as children. And if he does decide to dissolve the Obama-era program that provided some protections for them, there could be economic and legal complications across the country....
By Jonathan Blitzer - The New Yorker
Texass law, known as Senate Bill 4 (S.B. 4), is in some ways even harsher than the one Arizona passed seven years ago: the new measure allows state authorities to punish any police chief or sheriff who tells his or her subordinates not to act as de-facto immigration agents....
5/31: Under the Shadow of Trump, Dismantling Obamas Deportation Machine (2)
By Sarah Lazare In These Times
....As recently as this month, a federal judge ruled that Stewart can force-feed Vitaly Novikov, a 61-year-old detained Ukrainian man, who had been waging a hunger strike. The forced feeding of people incarcerated in Guantanamo Bay was condemned as torture by the United Nations human rights office in 2013....
5/31: Under the Shadow of Trump, Dismantling Obamas Deportation Machine (1)
Communities are fighting back against an immigrant prison in rural Georgia....
5/31: The Immigrant Crackdown Is a Cash Cow for Private Prisons
By Samuel Gilbert - Vice
Earlier this month, Daniel Ragsdale, the second-in-command at the Immigration and Customs Enforcement Agency (ICE), confirmed he will be leaving his position to work at GEO Group, the nation's second-largest private prison company....
5/29: Latinos are reporting fewer sexual assaults amid a climate of fear in immigrant communities, LAPD says
By James Queally Los Angeles Times
Los Angeles Police Chief Charlie Beck said Tuesday that reports of sexual assault and domestic violence made by the citys Latino residents have plummeted this year amid concerns that immigrants in the country illegally could risk deportation by interacting with police or testifying in court....
5/25: Advocates Call for Closure of Georgia Immigration Detention Centers Following Two Immigrant Deaths
By Detention Watch Network (DWN)
May 15th Jimenez-Joseph, 27, was found unresponsive after spending 19 days in solitary confinement at the Stewart Detention Center. ICE is reporting the death as a suicide. Following requests from the mother of Jimenez-Joseph, members of El Refugio, an organization that provides hospitality to loved ones visiting those detained at Stewart, attempted to visit Jean Carlos, but were turned away citing the conditions of his solitary confinement....
5/25: ACLU Sues to Expose Treatment of Hunger Strikers in ICE Detention
By Inga Sarda-Sorensen - ACLU
Reports of Hunger Strikers Being Force-Fed and Shunted Into Solitary Confinement Raise Serious Concerns....
2008 News
Massive Racist ICE Immigrant Raids Across the Country at Post-May Day 2008 Mobilization!!
5/12 URGENT! Up to 700 Arrested in Iowa Raid!!!!
By Citizens For Legitimate Government
Up to 700 arrests estimated in Postville raid 12 May 2008 (IA) Four Homeland Security buses with U.S. Immigration and Customs tags on them have entered the Agriprocessors Inc. complex. The buses, along with a trail of SUVs and vans with Minnesota license plates, arrived at about 11:45 a.m. Tim Counts, a Midwest ICE spokesman, declined to confirm where people who are arrested will be detained. Federal officials have leased the National Cattle Congress fairgrounds in Waterloo, but they declined to explain last week whether the property was being prepared for use as a detention center.
5/24: 900 nabbed in California state on immigration charges
Federal immigration officers arrested more than 900 people in California on immigration violations this month, almost half of them in Northern California, officials said.
5/28: County Jails Welcome Immigrants
The immigration crackdown is filling county jails across the country with immigrants who have been torn away from their jobs and homes. Tens of thousands of arrested immigrants are bedding down in county jails while they await court dates and eventual deportation.
As the immigration crackdown escalates, county commissions and sheriff departments are increasingly signing contracts with the federal government to house arrested immigrants. For the most part, county governments are eager to receive immigrants into their jails.
The per diem payments they receive from two federal agencies' immigration and Customs Enforcement (ICE) and U.S. Marshals Service (USMS)'re covering shortfalls in county budgets, funding the hiring of new deputies, and paying for jail expansion projects. Although some localities are complaining of jail overcrowding and a diffusion of law enforcement priorities, more and more local governments are cashing in on the immigration crackdown.
ICE is the agency of the Department of Homeland Security (DHS) responsible for the "detention and removal" of unauthorized immigrants. USMS is the Justice Department agency that transports federal prisoners, including arrested immigrants. Both agencies offer per diem payments'enerally much higher than those provided by local law-enforcement organizations, governments and private companies that house immigrants.
5/14: Some Detainees Are Drugged for Deportation
Immigrants sedated without medical reason.
The U.S. government has injected hundreds of foreigners it has deported with dangerous psychotropic drugs against their will to keep them sedated during the trip back to their home country, according to medical records, internal documents and interviews with people who have been drugged.
The government's forced use of antipsychotic drugs, in people who have no history of mental illness, includes dozens of cases in which the "pre-flight cocktail," as a document calls it, had such a potent effect that federal guards needed a wheelchair to move the slumped deportee onto an airplane.
"Unsteady gait. Fell onto tarmac," says a medical note on the deportation of a 38-year-old woman to Costa Rica in late spring 2005. Another detainee was "dragged down the aisle in handcuffs, semi-comatose," according to an airline crew member's written account. Repeatedly, documents describe immigration guards "taking down" a reluctant deportee to be tranquilized before heading to an airport.
2007 News
3/30 Tucson, AZ: 2 dead in attack on migrants
Gunmen ambushed nearly two dozen immigrants
and smugglers packed into a pickup truck early Friday, killing two
people and wounding at least one, authorities said >> Read
Racist Radio Show "Jersey Guy"
3/26 New Jersey: Boycoot Radio Host "Jersey
Guys" and their sponsors!
Please pass this list around not only in Jersey,
but all over the country. They are doing "Operation La Cucha
Gotcha." so I think we should do our own operation. Any company
that sponsors bigots should be boycotted >> Read
3/23 Newark, NJ: An Immigrant Segment
by Radio's 'Jersey Guys' Draws Fire
Craig Carton and Ray Rossi think mental illness
is hilarious and Asian-Americans are best mocked with sing-song
Chinese accents. The men, hosts of an afternoon radio show called
"The Jersey Guys" that is heard here on WKXW (101.5 FM),
favor adjectives for politicians that have to be bleeped out >>
1 Year after History March 25 "Grand
Macha" Los Angeles
3/24 Los Angeles, CA: Looking back, moving
Organizers of last year's huge immigrant rights
rally in L.A. reflect on successes and goals as they ready for Sunday's
anniversary events >> Read
3/25: Year one of the immigrant rights
Washington drags its feet while the rest of society
adapts to a new reality >> Read
3/25 Los Angeles,
CA: Turnout low, but immigrant rallies hopeful
Immigrant-rights rallies staged today to
commemorate the anniversary of last year's Los Angeles march and
to call for reform legislation were marked by low turnout and a
counterdemonstration denouncing immigration >> Read
2/22 Alert! Immigration raids
sweep through SoCal eateries!
In a nationwide sweep across 17 states
and the District of Columbia, immigration officials descended on
popular eateries like Hard Rock Café and Planet Hollywood,
arrested almost 200 undocumented immigrants working for a janitorial
company and filed criminal charges against its top three officials.
>> Read
2/22/07 New York, NY: Report
from NYU Counter College Republicans Find the Illegal
Immigrant event
Hundreds of other New York University
(NYU) students, their allies, and NYU IMC contributors to protest
the College Republicans Find the Illegal Immigrant
>> Read
2/20: DOJ audit found anti-terror
case data flawed
Federal prosecutors counted immigration violations,
marriage fraud and drug trafficking among anti-terror cases in the
four years after 9/11 even though no evidence linked them to terror
activity, a Justice Department audit said >> Read
2/21: Using Anti-Immigration Hysteria
to Promote the Anti-Choice Agenda?
Todays immigration debate extends beyond
the goal of limiting the rights and humanity of immigrants: Its
about controlling who may be considered an American >> Read
2/20: Which Babies Are Real Americans?
Yuki Lin , born on the stroke of midnight this
New Years, became the winner of a random drawing for a national
Toys R Us sweepstakes. The company had promised a $25,000
U.S. savings bond to the first American baby born in 2007.
However, Yuki lost her prize after the company learned that her
mother was an undocumented U.S. resident. Instead, the bond went
to a baby in Gainesville, Georgia, described by her mother as an
American all the way. >> Read
3/10 New Brunswick, NJ: Conference - Making
Comprehensive Immigration Reform real, fair and humane
Our topics will include legalization, education
issues, workers rights, due process, local ordinances, and
the creation of an Office of Immigrant Affairs >> Read
2/19 Vista, CA: Residents believe anti-immigrant
fliers were tossed from plane
Residents of a central Vista neighborhood said
they watched dozens of fliers with anti immigrant messages fall
from the sky like "snowfall" Thursday evening. Some said
they believe the papers may have been chucked out of the airplane
they also saw pass overhead at the same time >> Read
3/3-4 Ithaca, NY: Northeast United Students
Against Sweatshops Regional Conference at Cornell University
>> Read
2/20 New York, NY: Please endorse the
following letter to support Ester Young and her immigrant struggles
2/26-3/2 San Francisco, CA: Stop the Raids!
No New Guest-Worker Plan!
In response to the rash of raids that have been
affecting our communities, Bay Area immigrant rights organizations
are planning a week of action (2/26 to 3/2) and will protest in
front of Homeland Security's office at 630 Sansome street in San
Francisco every day from 11am to 1pm. >> Read
2/13 Oaklnand, CA: Thieves preying on
undocumented immigrants, police say
Oakland is witnessing a rising crime trend of young
"rat packs" preying on poor, often undocumented immigrants
who carry their cash with them, according to a new police report
>> Read
2/18 Florida: U.S. MUSLIM COALITION CALLS
A coalition of major Muslim organizations, today
called for a worldwide "rolling fast" in support of Dr.
Sami Al-Arian. Al-Arian is the former Florida professor who has
been on a hunger strike since January 21 to protest his detention
and treatment by federal authorities >> Read
2/19 Greensboro, NC: Students Rally Against
Anti-Arab Hate Crime
Students from colleges across North Carolina
rallied Feb. 2 against a recent hate crime committed against three
Palestinian students at Guilford College >> Read
1/20 Phoenix, AZ: New AZ
Law Bar on Minutemen Proposed
A proposed new law would make felons
out of members of the Minuteman Civil Defense Corps -- and probably
some neighborhood watch groups >> Read
1/1/07 San Antonio, TX: Tell
ICE: Release Palestinian Family NOW!
The Arab American Community Coalition(theaacc.org)
has just learned of an entire Palestinian family - the Ibrahims
- being held in jail in Texas while waiting an unjustified deportation
>> Read
2006 News
12/22: Tacoma, WA: Corporate-Runs
Immigration Jail Capacity Grows+More Profit!
Back on Sept. 30 the Seattle Times
that capacity of the Northwest Detention Center, the immigration
jail built on a Tacoma Tideflats Superfund site, was expected to
grow to 1,000 by January 2007 >> Read
12/19: ICE makes largest
repatriation flight to Africa in two years (ICE Press Release)
U.S. Immigration and Customs Enforcement
(ICE) officers returned from their largest deportation flight to
Africa in two years after repatriating 99 Nigerian and Liberian
nationals. This was a joint charter removal flight conducted with
the government of Canada.... >> Read
12/12: More Than 1,200
Arrested in Six-State Immigration Raid
to Locate/Help Immigrants Who Are Arrested/Detained
Immigration officials have set up
a toll-free telephone number, 866-341-3858, for families
to contact for information on where their relatives are being detained.
Operators speaking English and Spanish are available.
According to Democracy Now! radio
show and other reports, at least 1,280 workers have been arrested
in a series of immigration raids targeting meatpacking plants owned
by the company Swift at Tuesday, December 12th (see the map). The
raids took place in Colorado,
Nebraska, Texas, Utah, Iowa and Minnesota (1,
It marks the
largest sweep of its kind ever against a single company.
Homeland Security Secretary Michael
Chertoff justified the raids saying many of the detained men and
women were using false or stolen identities.
Map of Swift Plants Nationwide
Day to remember-Swift ICE raid (Movie)
Many immigrant activists had critized
the raid. David Bacon, the labor journalists says Tuesdays
(12/12) immigration raids on meatpacking plants werent
about curbing identity theft, they were about union-busting.
Many also raise skeptical
about the DHS/ICE's motivation of the raid. Many had pointed
out that only small numbers of the workers who arrested were charged
identity theft crimes.
The United Food
and Commercial Workers International Union has filed an emergency
lawsuit in an effort to release the workers who they say were illegally
detained and are being held without access to legal counsel. In
Iowa, immigration lawyers have accused the federal government of
holding the arrested workers at the military site Camp Dodge near
Des Moines.
Furthermore, many immigrants had been
brutally terrified by the racist immigrant raids. On Marshalltown,
IA, Breastfeeding baby's mom among those detained; at Hyrum,
UT, immigrant raid puts families in limbo; Immigrant rights
groups accuse ICE
raids had violated immigrant's civil rights.
More On The Recent
Massive Raids
The Mid-Term Elections and Immigration
Immigrant Legal Resource Center] The November mid-term election
was a repudiation of the Republican majority and a strong protest
against the status quo -- whatever the status quo voters were registering
their opposition to: the war in Iraq, Congressional corruption,
the economy, or terrorism. And this mid-term election was largely
focused on national, not local, issues.
Not A Wedge Issue: Republican leadership tried, but failed, to make
immigration the wedge issue that would ensure their continued control
of Congress after aggressively promoting an enforcement-only measure
(H.R.4437) as their solution to reforming our broken immigration
system. In fact, exit polling as reported by the Washington Post
found that fewer than one in three cited immigration 'as extremely
important in influencing their decision, and they only narrowly
favored Republican candidates. About six in 10 voters said that
they believe illegal immigrants working in the United States should
be offered a chance to apply for legal status'.. Democratic candidates
won support from 61 percent of those who backed a path to citizenship,
according to the poll.
Results: As a result of this election, aÿsignificant number
of candidates were defeated who, given their records, would have
supported anti-immigrant and anti-immigration measures in the new
110th Congress that will convene next January. Who were they? Minuteman
Randy Graf (R-AZ -- who ran for the seat of retiring Representative
Jim Kolbe); J.D. Hayworth (R-AZ), John Hostettler (R-IN -- the chair
in the 109th Congress of the House Immigration Subcommittee), Chris
Chocola (R-IN), Anne Northup (R-KY), Melissa Hart (R-PA), Bob Beauprez
(R-CO -- who lost his race for Governor), Charles Taylor (R-NC),
Gil Gutknecht (R-MN), and Richard Pombo (R-CA). Many of those defeated
are members of the so-called Immigration Reform Caucus (headed by
Representative Tom Tancredo (R-CO)) whose ranks have been reduced
by the results of this mid-term election. >>
11/10: Focus on Election
[Immigration News Briefs] Voters Reject
Many Anti-Immigrant Campaigns; Georgia: Republicans Gain, Voters
Split; Arizona Vote: the Good, the Bad and the Ugly; Colorado Vote:
More Ballot Initiatives; Election Results Elsewhere: A Mixed Bag
>> Read
11/10: In Arizona, Minutemen
lost more than House hopeful on Election Day
[Arizona Daily Star] Despite
the Minutemen's all-out effort to get Republican Graf into the congressional
seat vacated by fellow Republican Jim Kolbe, their man lost his
bid - in a Republican-dominated district - to Democrat Gabrielle
Giffords >> Read
Press Release: The
Surprise Winner of this Years Election Is
Immigration Reform by the National Immigration Forum
of 2006 Elections by the American Immigration Lawyers Association
Times: Bush Eyes Democrats for Help on Amnesty
The Hill: House GOP's immigration strategy
no magic bullet
10/18: Bush Signs the Detainee
Bill - Welcome to Martial Law?
Center for Constitutional Rights (CCR)
October 17, 2006
The Center for Constitutional Rights (CCR) denounced President Bush's
signing into law of the Military Commissions Act (MCA) on October
17, 2006. The final version of the bill emerged only four days before
the Senate's 11th hour vote. Although President Bush declared that
"time was of the essence" when he called for the legislation,
he has waited nearly two weeks to sign it into law. Congress has
once again been cowed into doing the President's bidding and abdicated
their Constitutional powers in the process, say attorneys.
The new law strips the right
of non-citizens to seek review of their detention by a court through
the filing of a writ of habeas corpus, the venerated legal instrument
that for centuries has protected people from arbitrary detention,
disappearance and indefinite detention without charge. The Act is
also meant to erase the hundreds of habeas corpus petitions that
CCR and others have brought on behalf of many of the 450 men being
held at Guantánamo Bay, a move already once denied by the
Supreme Court. >> Read
Articles, Analysis:
and A: Military Commissions Act of 2006 (Human Rights Watch)
Download the Report
Report on Migrant Deaths at the US-Mexico Border
10/17: Detainee
bill a step backwards (Denver Post Opinion)
10/18: America,
Welcome to Martial Law? (Media Monitors Network)
10/17 Civil Rights Attorney
Lynne Stewart Sentenced to
28 Months In Jail; Remains Free On Bail
Civil rights attorney Lynne Stewart is claiming
victory today in a case that
could have landed her in jail for the rest of her life. On Monday
(10/16), Stewart
was sentenced to twenty-eight months in prison. She'll remain free
on bail
while her conviction is appealed. >> Read
Listen to
Democracy Now! Radio Report
to National Immigrant Solidarity Network home page
to actionla.org


text:
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Anatoxin-a (ATX) is a potent neurotoxin produced by several species of
Freshwater toxins can be divided into three groups including microcystins and nodularin that produces hepatotoxic effects, anatoxin-a (ATX), homoanatoxin-a (HATX) and saxitoxins with neurotoxic effects and cylindrospermopsin that inhibits protein synthesis and induce oxidative stress [
Chemical structure of anatoxin-a (ATX).
Cyanobacteria-producing cyanotoxins have a global distribution and most of them are able to bloom in aquatic environments, posing human health and environmental risks. The largest part of the outbreaks associated with this increasingly occurring phenomenon is being observed in water bodies of North America, Australia and Europe [
FP is a suitable technique to study interactions between two molecules and therefore often used to develop biotoxins detection methods [
Effect of ATX-a on fluorescence polarization (FP) units (mP) of membrane-nAChR-F conjugate. 100 µM of ATX-a was added to a constant amount of membrane-nAChR-F conjugate. FP was measured after 60 min incubation at 37 °C and 24 °C. Data are means ± SEM of three experiments. (
Effect of ATX-a on FP units (mP) of membrane-nAChR-F conjugate. Different concentrations of ATX-a were added to a constant amount of membrane-nAChR-F conjugate. FP was measured after 60 min incubation. Data are means ± SEM of three experiments.
Effect of ATX-a (
Then, the FP method developed was tested with culture samples from three different cyanobacterial isolates,
Multiple Reaction Monitoring (MRM) chromatogram in positive mode (
Multiple Reaction Monitoring (MRM) chromatogram in positive mode of the
Positive multiple reaction monitoring (MRM) mode chromatogram and MS spectra of the 4.15 min peak, 5.90 min peak and 7.14 min peak from
Due to the different intensity found among ATX (
Pure ATX-a fumarate salt was purchased from Abcam® (Abcam plc, Cambridge, UK). Phosphate-bufffered saline solution (PBS) composition in mM: 137 mM NaCl (Panreac, Barcelona, Spain), 8.2 mM Na2HPO4 (Panreac, Barcelona, Spain), 1.5 mM KH2PO4 from Merk (Darmstadt, Germany), 3.2 mMKCl (Panreac, Barcelona, Spain), pH adjusted to 7.3 adding NaOH (Panreac, Barcelona, Spain). Methanol and acetonitrile were purchased from Panreac (Barcelona, Spain). Black 96-well polystyrene microplates, MicrotiterMicrofluor® 1 were from Thermo scientific, Hudson, NH, USA. Flat-bottom were used in all experiments.
Three cyanobacterial isolates were selected from the LEGE Culture Collection (CIIMAR, Porto, Portugal). Those include the anatoxin-a producing strain (ATX+)
To extract the toxins and minimize matrix effects an extraction protocol was employed. Twenty milligrams of lyophilised biomass were used for each one three
The receptor was used in PBS (pH = 7) composed of 130 mM NaCl, 1.5 mM NaH2PO4, 8.5 mM Na2HPO4, 0.1% Tween-20 (
A derivative of fluorescein, succinimidylesther of carboxyfluorescein (FAM), was employed as fluorescent molecule. Membrane-receptor labeling was performed using a kit purchased from emp Biotech: Fluoro protein 498 Spin Labeling and Purification Kit (Berlin, Germany) that includes all chemicals, tools, and dye reactive, needed for the labeling and purification processes. The membrane-nAChR was mixed with a sodium bicarbonate solution, then the dye was added and the mixture reacted for 1 h at room temperature and protected from light. In order to avoid unspecific interactions or unstable ester bond formation between dye and receptor, hydroxylamine was used as stop reagent. Finally, spin columns were used for rapid and efficient purification of the receptor-dye conjugate. With this kit, the dye reacts with an amine group of the protein and forms a covalent amide linkage. The membrane-nAChR-F conjugates have fluorescence-excitation and fluorescence-emission maxima at around 498 nm and 522 nm, respectively. The conjugate was stored at −80 °C protected from light until use 2.7 mg/mL of membrane-nAChR were labeled with the Fluoro Spin 498 protein Labeling Kit (emp Biotech GmbH, Berlin, Germany). The final membrane-nAChR-F conjugate, 90% of protein recovery, was dissolved in PBS. To select the correct dilution factor to perform the experiment and its volume, it is necessary to measure the fluorescence intensity of a dilution series of the protein solution obtained from the labeling reaction. Six different dilution factors, ranging from 1:100 to 1:7500 were tested in a final volume of 250 µL (data not shown). From this experiment, 1:1000 was chosen.With this dilution factor the final protein label concentration in each well was 4.8 ng/mL. This concentration remained constant in all experiments.
Changes in the fluorescence polarization (FP) of membrane-nAChR-F were measured by means of the Multi-Mode Microplate Reader, Synergy™ 4 from Biotek (Winooski, VT, USA). The instrument detection modes include fluorescence Intensity, FP, time-resolved fluorescence, luminescence, and UV-visible absorbance. Two types of fluorescence detection systems are available with Synergy 4, filter-based and monochromator-based. The instrument is equipped with dichroic mirrors and polarizing filters for FP. For measurement of FP and Fluorescence, excitation and emission wavelengths of 485/20 and 528/20 were used for membrane-nAChR-Fconjugates, respectively.
The polarization degree of the emitted light (measured in millipolarization units, mP) is calculated by the following equation:
The instrument was controlled using BioTek's Gen5™ PC software (BioTek® Instruments Inc, Winooski, Vermont, USA) for all measures, and data were exported to excel software for analysis. All results in this study are expressed as the mean ± SEM and the experiments were performed in triplicate with duplicate measurements per replicate. The results are expressed as fall of mP units (%) after toxin incubation.
The LC-MS/MS analysis was performed by a combination of HPLC plus mass detector. The HPLC system, from Shimadzu (Kyoto, Japan), consists of two pumps (LC-10ADvp), autoinjector (SIL-10ADvp) with refrigerated rack, degasser (DGU-14A), column oven (CTO-10ACvp) and system controller (SCL-10Avp). This system is coupled to a QTRAP LC/MS/MS system from Applied Biosystems, (Bedford, MA, USA), which integrate a hybrid quadrupole-linear ion trap mass spectrometer equipped with an ESI source. The nitrogen generator is a Nitrocraft NCLC/MS from Air Liquide (Madrid, Spain).
The column used for cyanotoxins separations was a reverse phase C18 analytical column (100 mm × 4.6 mm i.d.) Chromatolith® Performance RP-18efrom Merck, Darmstadt, Germany. The temperature was set at 40 °C. The composition of the mobile phase was: water (A) and acetonitrile (B), both containing 0.05% formic acid. Chromatographic separation was performed by gradient elution (23 min): starting with 2%–70% B for 12 min, then, 70% B was hold for 5 min and reducing afterwards to 2% B over 1 min and hold for 5 min until the next run. The mobile phase flow rate was 0.6 mL/min and the injection volume was 5 µL. Collision-induced dissociation (CID) in the ion-trap MS was performed. The electrospray ionization (ESI) source of QTRAP was operated with the following optimized values of source-dependent parameters: Curtain gas™ (Air Liquide, Madrid, Spain,): 20 psi, collision-activated dissociation gas (CAD): 6 psi, IonSpray Voltage: 4000 V, temperature: 450 °C, gas 1: 50 psi and gas 2: 50 psi.
The mass spectrometer was operated in multiple reaction monitoring (MRM) detecting in positive mode analyzing the following transitions: anatoxin-a (ATX) (
All experiments were carried out at least three times using duplicates. Data were normalized and results were expressed as means ± SEM. Results were analyzed using the Student
In this paper, an effective and rapid functional method to detect ATXs in samples of
The research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR.
From the European Union's Seventh Framework Programme managed by REA—Research Executive Agency
The authors declare no conflict of interest.


text:
Brooklyn Letters is presenting a multidisciplinary parent-friendly lecture on Autism Spectrum Disorders (ASD) in early childhood through the adolescence years tomorrow, February 7, at the Brooklyn Society for Ethical Culture.
The one-hour lecture will help parents understand the assessment process and diagnostic criteria of ASD. It will also explore the best ways to support a child with ASD, especially in school, social dynamics, and communication. Information will be presented by neuropsychologist Dr. Lindsay Whitman, child psychologist Dr. Carolyn Kessler, learning specialist and author Dr. Blythe Grossberg, and speech language therapist Craig Selinger (pictured above).
A Q&A with the experts will follow the lecture, and active audience participation is encouraged. Dr. Grossberg's book Asperger's Rules!: How to Make Sense of School and Friends will be raffled off for free to several audience members.
Brooklyn Society for Ethical Culture is located at 53 Prospect Park West, 7:15-9:00 p.m., $25 for person or $40 per couple, email Craig at email@example.com to reserve a spot.
Photo via Brooklyn Letters


text:
Charles Ebbets hired a new manager for 1906, Patsy Donovan, who had been a player in the majors since 1890 and a player-manager since 1897. With some strong pitching from Bill "Doc" Scanlan (18-13), consistent hitting by Harry Lumley (.324), and power from Tim Jordan (12 home runs, the most in the NL that year), the team edged back to fifth place. Their record, however, was still below .500 (66-86), and they ended up a full 50 games behind the pennant-winning Chicago Cubs, who boasted the famed Tinker-to-Evers-to Chance double-play combination and the amazing, if unorthodox, pitching of Mordecai "Three Finger" Brown. The Dodgers added a great pitcher of their own the following year when they signed 22-year-old rookie Nap Rucker. Rucker won 134 games for Brooklyn over the next 10 years and posted a career ERA of 2.42. Still, the Dodgers languished in the second division; in fact, they remained there until 1915, putting together a disappointing string of 12 years below the first division level. Patsy Donovan remained at the helm for only three years; that First year finish of fifth had turned out to be his best. He was followed by the one-year appearance of Harry Lumley, who could edge Brooklyn no higher than sixth, and then the four-year term of Bill Darleen, a shortstop who had been bouncing around the National League since 1891 (including a tour with Brooklyn from 1899 through 1903).
History and Information
In 1914 Robinson began what would be the longest tenure of any Brooklyn manager, 18 years, and a record which stood until Walt Alston came along 40 years later. With Uncle Robbie, still another nickname for the Brooklyn ball club emerged-no longer Grooms, Dodgers, or Superbas, they would now be called the "Robins." Robinson felt that he had a team whose talents were capable of producing a winner his maiden year, but those they just hadn't yet learned how to do it together. He was basically correct. They came in fifth in 1914, still playing below .500, but the good signs were there: their 75 wins that year were the most since 1902. Four players hit above .300. Jake Dauber's .329 was the league's best, and it was the second year in a row he had captured the NL batting crown. Average in 1913 had won him the honor of becoming the first Brooklyn player ever to lead tile league in batting, and the first to be named the National League's Most Valuable player.) His offensive efforts were bolstered by Zack Wheat and Jack Dalton, who both hit .319, and Casey Stengel, who batted .316. On the mound, jell Fifer turned in a sterling performance, winning 23 and losing only 12.The next year, 1915, Brooklyn broke into the first division, finally producing a record better than .500 (80-72).
Buy for all Dodgers schedule games at Dodgers stadium as well as Baseball dodgers road games.
The third place finish was the best since 1902.It was 1916, however, that became the year to remember at Ebbets Field. Uncle Robbie guided his Robins to their first pennant since 1900, and Brooklyn paid it's first-ever visit to a World Series. It had been a close race, with the Philadelphia Phillies applying the most pressure. Pitching was the Phil's' forte, with Grover "Pete" Alexander winning 33 games that year and Eppa Kixey 22. But Brooklyn prevailed by winning 94 games while losing only 60, outdistancing the Phillies by two and one-half games. In the American League, the Boston Red Sox came up a winner, largely because of the pitching of a 21-year-old left-hander in his third major league season: Babe Ruth had won 23 and lost 12 and had posted the lowest ERA in the league, 1.75. By 1920, the face of the Robins had changed, although not drastically. There were still several remnants of the 1916 pennant winners around Zack Wheat and Hy Myers in the outfield, Ivy Olson at short, Otto Miller behind the plate, and Jeff Pfeffer on the mound. But there were also some notable newcomers-Ed Konetchy at first, Pete Kilduff at second, Tommy Griffith in right field, and Grimes, Leon Cadore, and A1 Mamaux on the pitching staff. The blend was obviously a good one. Uncle Rohbie's squad of 1920 won Brooklyn its second trip to the World Series, breezing through the season on a record of 93-61, finishing seven games ahead of the Giants. Grimes won 23 games, losing only 11, his win percentage of .676 the best in the league that year. Zack Wheat led the Robins hitters with an average of .328, and Ed Konetchy and Hy Myers both hit above .300 as well. In addition, Mvers contributed 22 triples, the most in the league.
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