text:
Automation will make a significant impact on your business, so be sure you pick the right partner for your automation needs.
To be successful, pharmacists need fully integrated prescription-filling systems that provide the technology for higher levels of patient care and also help control costs.
At ScriptPro, we have more to offer than automation and technology solutions for your pharmacy. We back it all up with service and support to ensure that you get the most out of your investment.
ScriptPro provides innovative, seamlessly integrated, robotics-enabled systems to optimize retail and ambulatory pharmacy services.
Read the latest about ScriptPro and Pharmacy Industry News.
ScriptPro offers a number of security enhancements for our hardware and software systems-from electronic door locks for robotic systems to secure data storage and more.
SP Central includes multiple security configurations and features that cover:
Learn More About Workflow
Electronic Door Locks restrict and control user access to drug stock in ScriptPro Robotic Prescription Dispensing Systems.
To access the cabinet, users enter or scan an electronic PIN code, which immediately unlocks the cabinet doors.
The system tracks the date/time and user who opened the doors, and after a configurable amount of time, the doors auto-relock once they are closed.
Learn More About Robotics
Locking Cell Column limits access to the 25 cells located in Column A of the ScriptPro robotics cabinet.
When an operator requests access to the cabinet, the Cell Security Door covers and locks the cells unless the operator specifically requests access and presents proper security credentials.
This feature allows secure storage of any drugs that require additional control in the cabinet and allows date/timestamp and reporting of which operators have accessed the secure location.
Reinforced Doors provide robust added security for ScriptPro Robotic Prescription Dispensing Systems.
Using perforated metal, Reinforced Doors partially conceal drug stock and strengthen the cabinet door to deter break-ins.
Reinforced Doors are easy to open and provide sufficient close-up cabinet viewing for pharmacy staff.
Smart Card Scanner enhances system security by providing two-factor authentication to ScriptPro systems when coupled with a customer's Smart Cards.
It can be configured to lock the screen of a ScriptPro system upon removal of the Smart Card, thus protecting sensitive data and controlling access to Personally Identifiable Information (PII).
Learn More About ScriptPro Products
ScriptPro is PCI DSS v3.0 compliant, providing your pharmacy the option to store credit card numbers in order to bill for delivery or mailed prescriptions without requiring manual entry of the customer's credit card number.
To allow the operator to select the patient's preferred credit card, a masked credit card identifier is presented in the user-interface.
ScriptPro incorporates robotics, workflow, and pharmacy management software within a unified architecture. We call this Perfect Integration-the complete solution for your pharmacy, engineered and supported by ScriptPro.
Thank you for your interest in ScriptPro.
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A representative will contact you within 48 hours.
If you need immediate assistance, please call us at 800.606.7628.
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Dispensing medications should be a snap, not a headache!
ScriptPro's robotic dispensing systems safely and accurately fill 40-75% of your daily prescription volume, allowing you and your staff to focus on what really matters - patient care.
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Take the chaos out of your pharmacy. Our workflow system dramatically improves efficiency.
With the most streamlined design on the market, your workflow needs are met, and pharmacy operations will run better than ever before.
Our industry-leading pharmacy management system is fully integrated with IVR, point of sale, inventory, 340B management, and more-works on the same unified platform with our robot and workflow systems.
We call this Perfect Integration. One call, one connection, one company.
At ScriptPro, we have designed the safest, most advanced telepharmacy system in the world so your pharmacy can provide advanced patient care anytime, anywhere, and still maintain control over the entire dispensing process.
Serve patient needs around the corner or around the world with ScriptPro.
ScriptPro's 340B Management Package is a "real-time" advanced tool for managing your 340B programs.
Our 340B package identifies a prescription as 340B while the patient is in the pharmacy and makes sure you dispense the right drug.
Joining with TeleManager Technologies, ScriptPro's integrated IVR/workflow product is unique in the industry.
Patients have more information regarding their prescriptions' status and pharmacists know the exact status and location of every prescription.
SP Central's Point of Sale Package is fully PCI DSS v3.0 compliant.
It allows a pharmacy to operate its industry point of sale application from any ScriptPro workstation.
The net result is maximized operator and workspace efficiency.
ScriptPro's TPMS helps you follow the money! This valuable management tool lets you oversee all aspects of contracts, third party billing, and collection of third party prescription reimbursements, including 340B prescriptions.
As the population ages, pharmacists are looking for the best methods of providing healthcare to elderly patients and other residents in long-term care facilities.
Improved filling accuracy, less redundancy in the pharmacy, and better prescription tracking are all part of the ScriptPro solution.
Improve efficiency and communication with ScriptPro Health Messaging Services (HMS). This web-based messaging system gives staff and healthcare providers a tool to generate and review messages from any internet-connected computer.
Patients can take an active role in managing the time and location where they pick up their prescriptions by using ScriptPro's PSP. These self-serve patient pharmacy services include ticket queuing and patient/prescription integration.
ScriptPro and Eyecon® teamed up to integrate SP Central Workflow, SP Central Pharmacy Management, and ScriptPro Robotic Systems with the Eyecon tabletop tablet/capsule counting device.
This means accurate manual fills, double counts, and correct physical inventory.
ScriptPro brings TCGRx adherence packaging technologies to retail pharmacies throughout the U.S.
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166 Inter Stabilimenti Farina Berlinetta
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Ferrari's earliest production cars all shared common components that were first found on the Ferrari 125 S. Their exact specification and dimensions varied to create individual models. From 1948 two distinct model lines were available; the 166 MM and the 166 Inter. Although both names refer to famous race victories (the 1948 Mille Miglia and the Copa Intereuropa that same year), only the 166 MM was sold as a full racing car. With a milder engine and a longer wheelbase, the 'Inter' was considered a road car.
Both models sported a 2-litre version of the Gioacchino Colombo designed V12 engine. The 166 in the type name referred to the unitary displacement of the engine in 'cc'. The milder street spec used just one Weber Carburetor. This was sufficient to draw 115 bhp from the all-aluminium single overhead camshaft engine. By comparison, the triple Weber equipped 166 MM engine was good for around 140 bhp. The engine was mated to a five-speed gearbox that was also built in-house.
Ferrari's first chassis design was even more universally used in those early years as the engine. The chassis frame was constructed from two elliptical steel side-members with several cross-members for added rigidity. At the front the suspension consisted of double wishbones with a transverse leaf spring and hydraulic shock absorbers. The rear suspension was even more straightforward with a live-axle and longitudinal semi-elliptic leaf springs. As was inevitable in the day, braking was provided by hydraulic drums.
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text:
January 11, 2012
Dr. Jamie Aten is interviewed on Moody's This Is The Day program about the new Humanitarian Disaster Institute he co-directs at Wheaton.
January 9, 2012
The New York Times features the Leon Levy Expedition to Ashkelon, co-directed by Archaeology Professor Daniel Master.
January 8, 2012
Sociology professor Brian Miller is quoted on civil religion and perceptions of Tim Tebow for this article in the Pittsburgh Post-Gazette.
January 6, 2012
Politics and International Relations Professor Amy Black offers three keys to civil civic discourse in the cover story for the January 2012 issue of Christianity Today.


text:
Welcome to Arrow Wrecker Service
Click here to enter our website.
Copyright © Arrow Wrecker Service; all rights reserved. ~ Licensed and Insured ~ 700 N. Villa, OKC, OK 73107Phone: 405-943-1800 Fax: 405-236-3502 Toll Free for trucking companies: 866-505-2309Candace Muzny's Website: www.CandaceRacing.com
Another Great Site By Wheat Communications, LLC www.WheatCom.com


text:
2013 Capital One Academic All-District Team
BUIES CREEK, N.C. – Junior second baseman Shaela Churchill (Winterville, N.C.) was selected to the Capital One Academic All-District 3 team on Thursday as voted upon members of the College Sports Information Directors of America (COSIDA).
This is the third-straight year that Campbell has had a representative on the all-district team with Churchill joining Taylor McGee (2012), Sarah Forgacs (2011) and Lizzie Goldbach (2011) as honorees. Churchill is now on the ballot for the Capital One Academic All-America Team, which will be announced later this month.
The Winterville, N.C., native carries a 4.0 grade-point average, while working towards her degree in mathematics. The two-time NFCA Scholar Athlete has also earned acknowledgement on the 2012 Big South Presidential Honor Roll and 2011 Atlantic Sun All-Academic Team.
Churchill has posted a .250 batting avg. along with a double and two home runs on the season for the Camels. She has registered 10 runs scored and driven home four runs, while compiling a .933 fielding pct.
The Big South Conference also saw Winthrop's Kathryn Eberlin and Chelsey Shoenfeld and Liberty's Sammi Shivock earn a place on the academic all-district team.
Churchill is the seventh student-atlete to earn academic all-district 3 recoginition during the 2012-13 school year. Colton Burris (FB), Sam Eberwein (FB), Ben Iiames (MSOC), Kurt Odom (FB) and David Olson (BSB) have also earned the honor.
At least one Campbell student-athlete has been chosen to the academic all-district team in each of the last 19 years.
CoSIDA's 1800 members in the United States and Canada nominate players for Capital One Academic All-America® recognition. To be eligible, a student-athlete must be at least a sophomore in academic standing, have at least a 3.30 cumulative grade-point average, and be a starter or key reserve on his or her team. CoSIDA members vote on the all-district teams; then a national panel selects the Academic All-America squads from all first-team all-district choices. District 3 includes all NCAA Division I schools in North Carolina, Tennessee and Virginia.
The Camels wrap up the regular season against Longwood on Saturday beginning with a game on ESPN3 at 4 p.m. Free audio will be available for the second game of the doubleheader, while a video broadcast is provided for the finale on Sunday at 2 p.m. as Campbell honors seniors Brittany Arnn (Danville, Va.) and Forgacs (Cary, N.C.).
Pos. / Name / School / Yr. / Hometown / GPA / Major
P / Ellen Renfroe / TENNESSEE / Jr. / Jackson, Tenn. / 3.76 / Psychology
P / Emily Weiman / NORTH CAROLINA STATE / So. / Pasadena, Md. / 3.74 / Sport management
C / Chelsea Ingersoll / CHARLOTTE / Jr. / Charlotte, N.C. / 3.61 / Pre-biology
IF / Raven Chavanne / TENNESSEE / Sr. / Thousand Oaks, Calif. / 3.84 / Political science
IF / Shaela Churchill / CAMPBELL / Jr. / Winterville, N.C. / 4.00 / Mathematics
IF / Haley Pace / WESTERN CAROLINA / Sr. / Canton, N.C. / 3.98 / Biology
IF / Sara Poteat / TENNESSEE, CHATTANOOGA / Sr. / Thomaston, Ga. / 3.80 / Exercise science
IF / Madison Shipman / TENNESSEE / Jr. / Valencia, Calif. / 3.51 / Anthropology
OF / Allie Cashion / APPALACHIAN STATE / Sr. / Waxhaw, N.C. / 3.67 / Actuarial science
OF / Jill Jelnick / EAST / CAROLINA / Jr. / Aliso Viejo, Calif. / 4.00 / English
OF / Leah Taylor / TENNESSEE, MARTIN / Sr. / Brownsville, Tenn. / 3.97 / Marketing
DH / Sammi Shivock / LIBERTY / Jr. / New Castle, Del. / 3.81 / Sport management


text:
LEXINGTON, Ky. – Kentucky coach John Calipari doesn't appear to be too worried about all the attention freshman center Nerlens Noel has been getting this summer.
There were questions about Noel's academic standing after he reclassified during recruitment to move up a year. Noel was cleared academically by the NCAA and started classes in late August. Then there was an SI.com report that the NCAA was expanding its inquiry into Noel's amateur status.
Noel was unable to answer any questions regarding the NCAA, but Calipari seemed optimistic Thursday when asked about the uncertainty surrounding Noel and the possibility of him playing this season.
"We feel comfortable," Calipari told USA TODAY Sports. "It's a review that those kinds of players go through. When you reclassify, you're going to have reviews. When you reclassify and some people are mad you reclassified, there are going to be some reviews. That's how it is. We're comfortable that he's done everything he was supposed to do."
Noel, one of the top recruits of the 2012 class, is a 6-11 center from Everett, Mass., who played for Tilton (N.H.) Prep last season and was the USA TODAY Sports high school player of the year. Some analysts expect him to be a better shot-blocker than Anthony Davis, who blocked 186 shots and swept all of the national player of the year awards last season before being the No. 1 overall pick in the NBA draft.
Noel chose Kentucky over Georgetown and Syracuse.
First-place votes in parentheses.
Football coaches' poll
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text:
Washington, D.C. – U.S. Senators John McCain (R-AZ) and Jeff Flake (R-AZ) today released the following statement on the latest reports of mismanagement and neglect in the Phoenix VA Health Care System:
"We are outraged and saddened by the latest reports of mismanagement and neglect in the Phoenix VA Health Care System. It is unconscionable that officials at the Phoenix VA reportedly would allow a level of systemic failure that resulted in delays linked to the deaths of up to 40 veterans who were awaiting medical care, and then allegedly tampered with documents to cover it up. To get to the bottom of these whistleblower reports, which our offices have been actively investigating for months, we request that the Senate Veterans Affairs Committee initiate an investigation and hold hearings as soon as possible. Our nation has a duty to provide the best quality of care to those who have served and sacrificed on our behalf, and we must hold to account those responsible for breaking faith with that solemn obligation."


text:
Are you searching for the next branded giveaway to top off your marketing strategy? Office workers want items that are easy to use and that make their lives behind the desk a little easier. Lend a helping hand with memo clips to help remind them of the day's to-dos! At ePromos, we carry magnetic memo holders and branded bag clips to keep users organized while increasing your brand's overall visibility. Enjoy a perpetual advertising spot within your client's workspace using memo holders customized by ePromos.
Perfect for reminders, memos and a photos! This imprinted custom clip has magnetic back and spring loaded hinge.
Open bottled beverages with these promotional bottle openers that double as a magnetic memo clip.
The magnetic promotional clip has a strong magnet for super holding power!
Write a memo to hang on your fridge, or close up a bag of chips with this Color Grip Magnetic Custom Chip Clip w/ Pen! This versatile clip comes in 4 fun colors!
Take a bite out of success with this apple shaped magnetic power custom imprinted clip!
This fun promotional clock is a handy way to keep on time and ieven hold memos!
An efficient way to clip notes to refrigerators or filing cabinets with our personalized clip.
Translucent Note Nest logo note holder.
A logo imprinted memo clip that is perfect for new home owners.
Get a grip with this logo memo clip!
Think outside the box by leaving memos on this cubed promotional memo holder.
Get a grip with this star shaped promo memo clip!
You will love the usefulness of this heart shaped custom imprinted memo clip!
Show some love for your customers with these heart shaped magnetic custom clips.
Get a grip with this Full Color Magnetic Promotional Memo Clip. Great for holding your papers, coupons lists, children's artwork and also functions as a chip or snack bag clip.
Put your note or message outside the box with this custom imprinted memo holder.
At almost 5" long, the Bamboo Promotional Memo Clip is environmentally friendly and a stylish desk accessory.
Translucent vinyl promotional memo holders is the perfect office desktop accessory.
Bring your promotions home with this super strong, house shaped custom magnet clip.
Send a thoughtful message with this handy promotional bag clip in the shape of a heart.
Handy heavy-duty promotional clip made of injection molded plastic tightly seals bags to keep your food fresh or holds your documents together to keep organized.
A handy Keep-It Clip Custom Logo Bag Clip that is sure to be used again and again!
Keep your brand growing with this flower shaped promotional memo holder.
Keep your logo in front of your recipient with these promotional binder clips.
This cube shaped liquid wave promotional memo holder will bring attention to any logo or message.
Creative logo computer monitor note clip for holding documents, notes, and photos easily to either side of a computer monitor.
Get your company logo out to people's desktop first with this cool steel promotional memo holder with multiple clips.


text:
Geoff Hutchison (email@example.com)
Sun, 24 Jan 1999 13:54:54 -0400
At 4:34 PM -0400 1/22/99, Doug wrote:
>> But the majority of our users would really like to be able to find
>>documents containing "polymères", without having to type the accented
>>character(s). Currently, htsearch finds no documents on "polymeres", and
>>that unacceptable for our users.
>> Is there anything you can suggest for this situation?
> Off hand I'd suggest that you use the synonym database, but I wasn't
>able to find real clear documentation on how to do that.
At the moment, this may be the best solution. We have several people
working on internationalization, including an "ASCIIfy" system where you
could translate the accented words to ASCII. Contact Iosif Fettich
<firstname.lastname@example.com> for more information.
Using synonyms is pretty easy. Edit the synonym file in the common
directory (the file currently in there is really a suggestion) and run
htfuzzy synonyms and you should be set. (My rundig script actually keeps
tabs on the synonym file and runs htfuzzy when necessary.)
-Geoff Hutchison
Williams Students Online
To unsubscribe from the htdig mailing list, send a message to
firstname.lastname@example.com containing the single word "unsubscribe" in
the body of the message.
This archive was generated by hypermail 2.0b3 on Mon Jan 25 1999 - 08:15:24 PST


text:
Grossing a massive $70.2 million on its debut weekend, the Dr. Seuss animated film marks the biggest opening ever for a non-sequel animation and the greatest opening in 2012 so far.
Universal Pictures' environmental animated movie "The Lorax" secures the champion title on North American box office with a massive gross on its opening weekend. The family film based on Dr. Seuss' children book grabs a massive $70.2 million, which is far above the studio's expectation.
The satisfying result marks the biggest opening ever for a non-sequel animated pic, breaking the record previously held by Pixar's "The Incredibles" which opened to $70.5 million in 2004. "Lorax" also marks the greatest box office performance by any animated film since 2010's "Toy Story 3", which debuted to $110.3 million, and "Shrek Forever After", which opened to $70.8 million.
"Lorax" scores the fourth top opening ever for any Universal's movie. Additionally, the Zac Efron and Taylor Swift-starring movie breaks the record of greatest opening in 2012, besting the $41.2 million scored by "The Vow" last February.
"The result is phenomenal," says Universal president of domectic distribution, Nikki Rocco, of the animation which production cost below $70 million. "Word of mouth was great, and that's why Saturday was up 80% from Friday. It's just spectacular."
Receiving an "A" CinemaScore, "Lorax" attracts to mostly parents and kids under the age of 12, which make up 70% of its ticket buyers. "I think there was a huge desire to see a Dr. Seuss 3D film from the creators of 'Despicable Me'," Rocco says.
Opening on the runner up spot is rave party film "Project X". The Warner Bros. Pictures' found-footage movie bags $21 million despite the fact the the film cost a modest $12 million. Receiving a "B" CinemaScore, it appeals to 67% moviegoers under the age of 25 with 58% of them are males. "[Producer] Todd Phillips is just incredibly talented," Warner Bros. executive vice president of distribution Jeff Goldstein said of the R-rated film.
Former chart-topper "Act of Valor" falls to the third place with $13.5 million, dropping 44% from last week. Denzel Washington's action pic "Safe House", meanwhile, stays on the fourth position this week with $7.3 million, dropping only 34% from last week. Rounding up the top five is "Good Deeds", which adds $7 million to its domestic cume.
*On the other hand, Oscar's big winner "The Artist" finishes on the tenth place with $3.6 million, increasing 34% from the prior weekend. The acclaimed silent drama, which cost a mere $15 million to produce, collects a total domestic cume of $37.1 million after fifteen weeks of playing in theaters.
Top Ten Movies at Box Office for March 2-4, 2012:
- "The Lorax" - $70.2 million
- "Project X" - $21 million
- "Act of Valor" - $13.5 million
- "Safe House" - $7.3 million
- "Good Deeds" - $7 million
- "Journey 2: The Mysterious Island" - $6.5 million
- "The Vow" - $6 million
- "This Means War" - $5.5 million
- "Ghost Rider: Spirit of Vengeance" - $4.5 million
- *"Wanderlust" - $3.8 million


text:
A former inmate and his nephew plan to kidnap and castrate the singer while he is having a show in New York City, along with his bodyguard.
News about Justin Bieber's murder plan emerged last year. Recently, a phone conversation containing details of the failed murder has been revealed. The master mind, Dana Martin, was an inmate who had two life sentence because of rape and murder to a 15-year-old girl. He ordered a former inmate Mark Staake, who recruited his nephew Tanner Ruane. Martin made the call in a New Mexico prison, where all calls were recorded and monitored.
In the phone conversations, Martin was heard talking to Ruane, "Did he go over the Bieber thing with you?" Ruane replied, "The way I like to work dude, I like to know as little as possible. He's gonna use one of the things you gave him, and then he's gonna take care of it." Ruane told Martin that he had buy a garden shear. It was assumed to be used to mutilate the victims' body.
Martin and Ruane talked in codes, "He's going to kill the pitbulls, and you are gonna castrate them." According to the authority, pitbulls refer to Bieber and his boydguard. Ruane was going to suffocate him with Martin's signature tie, and then mutilate his body.
Martin even instructed the details of how to make the tie, "Tie it really really tight. Knot it in the front once, really tight, put it in a knot. That cuts off the oxygen, and then tie it in the back again, really tight. That seals the deal."
The uncle and nephew traveled from Vermont to New York, when Beiber was having a show in Madison Square Garden. However, before the two men could execute their plan, they were stopped by border patrol because they took a wrong way which headed to Canada. The uncle and nephew demanded USD 5000 for each person they killed.


text:
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|Sunday April 14, 2002
On the eve of the US Masters, Thomas Bonk pays tribute to Bobby Jones, creator of one of the most respected courses and revered tournaments in world golf.
Bobby Jones died more than 30 years ago, but something he left behind still manages to summon vivid memories of golf's greatest player. The Masters begins on Thursday at the course he carved out of an old nursery on hilly, rolling terrain in Augusta, Georgia.
It's still Bobby Jones's tournament, fully grown into one of golf's most treasured and revered institutions - much like Jones himself.
Jones attained an unusual status in sport, as an icon, and maintains it more than three decades after his death. Many in the game feel about Jones the same way Arnold Palmer does.
"Bobby Jones was a real gentleman golfer," Palmer said. "What he accomplished in golf, no one else has and probably never will. He's also the father of Augusta National and the Masters, and all those factors are significant to his enduring popularity."
How Jones has endured is a story - his own story, actually - of achieving great fame through heroic achievements, showing the best examples of sportsmanship and humility, getting out of the spotlight early and dying too young.
According to Jones's biographer, Sidney L. Matthew, Jones knew exactly what he was doing when he stopped playing regularly in 1930 at the age of 28.
"He had the confidence and grace to leave the stage at the top," Matthew said. "That's something very few people can do."
The Jones legacy is storybook stuff, but also powerful. Hootie Johnson, chairman of Augusta National, invokes Jones's name often. "The spirit of Bob Jones is alive today," Johnson said. "It is evident here at Augusta National and in the Masters, in his outstanding player record and the decency and honesty he brought to the game of golf. He is missed by those that knew him and those who didn't."
Robert Tyre Jones jnr was born on St Patrick's Day, March 17, 1902. As a child he was frail and sickly, the result of a gastrointestinal disorder, the same ailment that killed his younger brother William at three months.
But Bobby Jones, whose family was far from impoverished, was able to enjoy some of the privileges of wealth. His grandfather was a successful entrepreneur and his father, Robert Purmedus Jones, an attorney who also excelled at sport, especially baseball. He became general counsel to an emerging local company that seemed to have promise. It was called Coca-Cola.
Young Rob was attracted to golf almost as soon as he could walk, eventually playing at East Lake Golf Club in Atlanta.
It didn't take long for him to prove his skill at the game - he won his first cup in a tournament when he was six. At 18, in 1920, he won the Southern Amateur in Atlanta, was a semi-finalist at the US Amateur and tied for eighth at the US Open.
Jones had graduated from high school at 16 and from Georgia Tech in 1922 with a degree in mechanical engineering.
He excelled off the golf course nearly as much as on it. He earned his second degree, in English literature, at Harvard. He took two semesters of law at Emory University then passed the bar exam on his first try.
Said Matthew: "Jones could be just as conversant in discussing The Heart of Midlothian by Sir Walter Scott as studying a putt on the golf course. He was the most intellectually brilliant player who ever played the game, no question.
"Before Jones, golfers were mostly former caddies and not very well thought of. The players were not educated. Jones changed the perception and set the standard."
Jones never turned professional and was basically a part-time golfer.
On the golf course, he was uniformly brilliant. His playing career was like a huge, bright comet that blazed across the sky and captured everyone's attention and then was gone too soon.
Jones competed in major championships for 13 years, and for nine of those years he was either a high school or college student. He was a part-time player and full-time genius. He played in 52 tournaments during that period, an average of four a year, and won 23.
Jones was 28 when he retired from regular competition in 1930. He had won 13 of the 21 majors he had played from 1923-1930: five US Amateurs, four US Opens, three British Opens and one British Amateur.
Even when he didn't win, he was close. In 11 of his last 12 USOpens and British Opens, he was either first or second.
His greatest achievement in golf took a year. In 1930, Jones won all four of what were at the time considered the biggest tournaments in golf - the US Open, the US Amateur, the British Amateur and the British Open. It was the grand slam. No-one had ever done it and, although Tiger Woods's achievement of holding all four major titles at the same time is stunningly remarkable, Woods has not matched Jones's record of winning all four of golf's biggest championships in the same calendar year.
Even more than 70 years later, the sporting public is enthralled by dominant athletes. The fact that Jones actually lived up to his image as a sportsman of the highest degree only enhanced his already considerable reputation.
One of Jones's playing partners was Charlie Yates. He is the only person still living who played in the first Masters, in 1934, a tournament Jones and Augusta National partner Clifford Roberts organised for their golfing friends.
Yates says Jones made the right decision in 1930 when he decided to retire.
"I think what he did was so smart," Yates said. "Having conquered all worlds, he retired, because he loved to play with his friends."
Once retired, Jones turned his attention to creating a golf course. He enlisted famed architect Alistair MacKenzie to design a layout that would suit players of all abilities, a risk-reward type of course. And that is just what Augusta National has represented since its formal opening in January 1933.
The first several tournaments were called the Augusta National Invitational, not the Masters, because Jones considered such a name pretentious, but he changed his mind after urgings from Roberts. Jones played in the first 12 Masters, although he never finished higher than 13th, his place in the inaugural event.
Jones said he never really missed tournament play.
"Golf is like eating peanuts," he once said. "You can play too much or too little. I've become reconciled to the fact that I'll never play as well as I used to."
His last round of golf was in 1948 at East Lake Golf Club. Yates had played with Jones a few weeks earlier.
"I recall quite vividly and sadly how one day in 1948 Bob said that he had trouble getting the club down from the top of his swing because of the pain in his back."
Only 46, Jones was stricken by syringomyelia, a neurological spinal disease. Jones did not give in to self-pity. He once said he would not accept "this thing", as he referred to his rare ailment.
"I fight it every day," he said. "When it first happened to me I was pretty bitter, and there were times when I didn't want to go on living. But I did go on living, so I had to face the problem of how I was to live. I decided that I'd just do the very best I could."
Jones underwent spinal surgery in 1948 and in 1950 and was forced into a wheelchair. He never saw the Masters after 1967.
In October 1952 he had a heart attack but was well enough three months later to attend a ceremony in his honour, at which a letter from one of Jones's close friends, an Augusta National member, was read. It was from President Dwight Eisenhower.
"Those who have been fortunate enough to know him realise that his fame as a golfer is transcended by his inestimable qualities as a human being," the letter said. "His gift to his friends is the warmth that comes from unselfishness, superb judgement, nobility of character, unwavering loyalty to principle."
It is said Jones wept openly.
He died on December 18, 1971, in Atlanta.The 66th Masters will be played this week at Augusta National. Jones would have been 100 years old.
Los Angeles Times
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In this section
The woman who won Tiger's heart
Mastering a harder course
Arnie, with his four green jackets, calls it a day
Flower power helps Scott escape
First round to course on points
Izawa warms up with consecutive aces
Love and loathing at golf's greatest event
Time for Australian pair to turn early promise into major results
Wham, bam, thank you Sam!
Duval chases a fairytale ending
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#1: John Richels
Company: Devon Energy
Compensation: $18.8 million
Company Net Income: -$206 million
Stock Return: -14.9%
The case of our highest paid head honcho at a money-losing company is a prime example of one word: perspective. In 2012, Devon Energy (DVN) CEO John Richels took home nearly $19 million in his second full-year on the job, all while the company lost around 10 times that — a total of $206 million in the red, or 52 cents per share.
Then again, when you consider that Devon posted a net loss of $4.7 billion back in 2011 — yes, an eye-popping $11.25 per share — the 2012 performance really doesn't seem all that bad. Either way, though, one thing is clear: DVN is struggling big-time in the face of falling natural gas prices.
Unlike other entries on this list, Devon hasn't posted an eye-popping comeback in 2013 either. Year-to-date, the stock's up a shrugworthy 5%, leaving its share price around 40% from 2011 highs.
Hopefully, the company's latest plan to spin off some of its pipeline and storage facilities will indeed unlock value for shareholders … and maybe make Richels' pay seem worthwhile.
As of this writing, Alyssa Oursler did not own a position in any of the aforementioned securities.


text:
James Buchanan RIP
He was a pioneering figure who left an indelible mark on the economics profession. He was a close friend of the National Center for Policy Analysis, and he participated in a number of NCPA events. He and his colleague Gordon Tullock were a huge influence on my PhD dissertation at Columbia and then on my subsequent work on public choice economics with Phil Porter.


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Indybay FeatureRelated Categories: Peninsula | Police State and Prisons View other events for the week of 6/ 6/2013
Comments (Hide Comments)
Wednesday Jun 5th, 2013 1:31 PM
what about police harassment and so called civil liberties. palo alto cops are notorious liars as the anarchos know. people are being snooped on by palo alto police. watch out. palo alto is a militar state testing ground.
Thursday Jun 6th, 2013 8:19 AM
Today's news story around the world, including the local daily rag, is
NSA collecting phone records of millions of Verizon customers
Exclusive: Top secret court order requiring Verizon to hand over all call data shows scale of domestic surveillance under Obama
by Glenn Greenwald, June 5, 2013
His article has the link to the court order.
Of course, if you are a serious voter, you did your homework in 2008 and learned that Obama is from a CIA family, whose parents met in a Russian language class in 1960 in Hawaii, at a time when if you were not of Russian descent, you did not take Russian as we were experiencing the anti-Soviet terrorism of the US government, of which the CIA is a part. You would also know that Obama owes his education to the CIA and his CIA history is why he called Mr. CIA, George Bush, Jr., a "good man" at the dedication of the Bush library.
You also read the article by then Green Party member, SF Board of Supervisors president Matt Gonzalez,
The Obama Craze, dated 2/29/08
The fact is the Democrats only exist to make sure we never vote Red (socialist) or Green. That is why they mouth a few decent phrases at election time to get your votes, and then once elected, carry out the same capitalist agenda as the Republicans because the Democrats and Republicans are paid for by the same capitalist class and their corporations. Obama's chief sponsor is billionaire Democrat Penny Pritzker, known for stealing workers' money at Superior Bank and union busting at her Hyatt hotels. He just rewarded her with a position as Commerce Secretary. No millionaire-billionaire can represent the workingclass. Obama is a millionaire. The Democrats and Republicans proudly state that they have NO DIFFERENCE on foreign policy, and since we cannot have guns and butter, they have no difference on domestic policy. There are lots of anti-abortion and anti-gay marriage Democrats.
The list of grievances above does not include the primary reason that people vote for the Democrats: Social Security and Medicare. Obama wants to cut Social Security and Medicare.
It also does not include his attacks on medical marijuana users, which means he is a proud promoter of the racist prison pipeline to maximize the profits of the prison industry, the only reason any drugs are illegal as all drugs should be legalized, getting them off the street as they will have no market value. Of course, if drugs are legal, then the CIA and the war machine will not have enough money to finance their blood for oil wars to maximize the profits of the oil and munitions industries where the greatest profits are; maximization of profits being the primary goal of capitalism. The only reason the military exists is to maximize profits of the capitalist class.
It also does not include the privatizing of the school system with Race to the Top, the same as No Child Left Behind, with the mass closure of public schools by Democrats in Chicago and across the nation in the workingclass, primarily African American communities. Clearly, the capitalist class wants to make this country another low wage, illiterate Haiti to maximize its profits.
The two parties on the California ballot which oppose spying, the CIA, the prison pipeline, illegal drugs (all drugs should be legalized), privatizing the school system, and cuts to Social Security and Medicare are Peace & Freedom and the Green Party. They need you now to stay on the ballot. Register in either party now online at:
For more information, see:
Thursday Jun 6th, 2013 8:29 AM
Democrat Obama has been trying to prevent the Morning After Pill from being non-prescription. We finally won a court order allowing it to be sold over-the-counter while Obama's Justice Department appeals the original court order doing the same. See
This is the same Justice Department that failed to drop Bush's frame-up charges against attorney Lynne Stewart but has continued the prosecution and persecution of this outstanding workingclass attorney and is now refusing to give her a compassionate release from prison as she is dying of cancer. Sign the petition to have her released at
For more information on her case, see
And this is the same Obama whose military is prosecuting and persecuting the heroic Bradley Manning who exposed American war crimes.


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This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Radix Angelica Sinensis (RAS) and Rhizome Ligusticum (RLC) combination is a popular herb pair commonly used in clinics for treatment of blood deficiency syndrome in China. The aim of this study is to compare the pharmacokinetic properties of ferulic acid (FA), a main bioactive constituent in both RAS and RLC, between normal and blood deficiency syndrome animals, and to investigate the influence of compatibility of RAS and RLC on the pharmacokinetic of FA. The blood deficiency rats were induced by injecting 2% Acetyl phenylhydrazine (APH) on the first day, every other day, to a total of five times, at the dosage of 100, 50, 50, 30, 30 mg/kg body mass, respectively. Quantification of FA in rat plasma was achieved by using a simple and rapid HPLC method. Plasma samples were collected at different time points to construct pharmacokinetic profiles by plotting drug concentration
RAS and RLC are both famous Chinese herbs from the Umbelliferae plants. They contain many aromatic acids and phthalides as major bioactive constituents. The aromatic acids, including among others ferulic acid, chlorogenic acid, caffeic acid, protocatechuic acid, vanillic acid, have antioxidant, antimicrobial, anti-inflammatory, and anti-thrombosis activities [
The pharmacokinetics of FA
RAS and RLC, especially the herb pair RAS-RLC, were clinically used for treatment of women's ailments resulting from blood deficiency in TCM. In the herbs, FA was found to exhibit significant effects on syndrome of blood deficiency [
Normal rats are strong, vigorous, with brilliant pink eyes, clean pink moist nose and lips, round pink tail, straight back and loin, their fur is luxuriant and lustrous. Three days after APH administration, the animals appeared exhausted, sluggish, body curled into a ball with raised hairs, asthmatic and somnolent, tail, face, ears and eyes pale and cool. The signs of blood deficiency rats matched the same description of blood deficiency symptoms.
Additionally, the periphery blood indexes in normal and blood deficiency rats are shown in
Under the conditions described above, the typical HPLC chromatograms of blank plasma, blank plasma spiked with FA and IS (IS: cinnamic acid; the chemical structures of FA and IS were given in
The peak area ratio of FA to IS
The validated method was successfully applied to the pharmacokinetic study of FA in rat plasma after oral administration of RAS, RLC, and RAS-RLC extract. The mean concentration-time curves are shown in
Acetyl phenylhydrazine is a strong oxidant, which has a slowly progressive and oxidative damage effect on RBC, especially interfering with glucose-6-phosphate dehydrogenase in RBC, promoting the changes from hemoglobin to Heinz-body, and making the RBC disintegrate easily, which finally results in hemolytic anemia of the body. The number of RBC then decreases and the content of HGB reduces significantly. As the number of WBC and rRBC pathologically increase, the liver and spleen swells and their color changes to dark red. All the results conform to the essential features of blood deficiency syndrome. It has been a standard procedure to induce rat blood deficiency and it is recorded in several textbooks [
Diagnosis and treatment based on an overall analysis of the illness and the patient's condition is the essence of TCM theory. Rational drug therapy is dependent upon a basic understanding of the way patients handle drugs (pharmacokinetics) and their response to specific drug effects (pharmacodynamics). Many researches have demonstrated that disease condition will cause the alterations of pharmacokinetic parameters [
In the present study, the pharmacokinetic profiles of FA were evaluated and compared after oral administration of RAS, RLC, and RAS-RLC extract. Results showed that the pharmacokinetic parameters of FA after oral administration of RAS-RLC extract were significantly different between normal and blood deficiency rats. The plasma drug concentration-time data of FA in normal and blood deficiency rats were best fitted to a two-compartment open model, coinciding with the results of other researchers [
The results in
Furthermore, the results in
The radix of
The dry herb materials of RAS (2 kg) and RLC (2 kg) were crushed to pieces, extracted with boiling water (1:8) for twice, 2 h for each time, filtered through gauze. The residue was refluxed with 95% ethanol once under the same conditions. Then three filtrates were merged and evaporated by rotary evaporation under vacuum at 60 °C, thus RAS and RLC extract samples were obtained, respectively. The concentration of FA was 0.46 mg/g in the extract of RAS and 0.51 mg/g of RLC. A total 2 kg mixed pieces of RAS-RLC (1:1, w/w) were extracted through the same procedure. The concentration of FA in the extract of RAS-RLC was 0.52 mg/g.
Male and female Sprague-Dawley (SD) rats (180–220 g) were obtained from Shanghai Slac Laboratory Animal Co. Ltd. (Shanghai), and kept in an environmentally controlled breeding room (temperature: 20 ± 2 °C, humidity: 60 ± 5%) for 1 week before the experiments started. The rats were fasted for 12 h with free access to water prior to the experiments. Animal welfare and experimental procedures were strictly in accordance with the
The reference standard of FA (98%) and internal standard (IS), cinnamic acid (98%), were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China).
Experiments were performed on 18 female and 18 male Sprague Dawley (SD) rats randomly divided into six groups. Three normal control groups and three blood deficiency model groups were administrated of RAS, RLC, and herb pair RAS-RLC extract, respectively. The blood deficiency model groups were given hypodermic injection with 2% APH on the 1st day, every other day a time, and total of five times at the dosage of 100, 50, 50, 30, 30 mg/kg body mass, respectively [
On the 10th day, the animals were given intragastrically RAS, RLC, and RAS-RLC extract dissolved and dispersed homogeneously in ultrapure water. The animal dose of RAS, RLC, and RAS-RLC extract was extrapolated from the human daily dose, using the body surface area normalization method [
The thawed plasma samples (100 μL) were transferred to a 1.5 mL Eppendorf centrifuge tube, added with 20 μL IS (0.37 mg/mL) and then mixed with 50 μL 0.1 mol/L hydrochloric acid and 850 μL ethyl acetate. Afterwards mixture was vortexed for 1 min and centrifuged at 3000×
Analysis was performed on a Waters 2695 Alliance HPLC system (Waters Corp., USA) equipped with a 2996 photodiode array detector (PDA) together with a quaternary pump solvent management system, an on-line degasser and an autosampler. The analytes were separated on a Hedera™ ODS-2 column (250 mm × 4.6 mm, 5 μm) from Jiangsu Hanbon Sci and Tech Co. Ltd. (Jiangsu, China). The mobile phase was composed of solvent A (0.3% aqueous glacial acetic acid,
Primary stock solutions of FA and IS were prepared by dissolving accurately weighed FA and IS in methanol to yield a final concentration of 0.219 and 0.37 mg/mL, respectively. For the calibration curve, six concentrations (0.1095, 0.5475, 1.095, 2.19, 4.38, 10.95 μg/mL) of FA solution were prepared by dilution of the stock solution. The serial solutions (10 μL) were spiked in 100 μL blank plasma, then 20 μL of the IS solution were added. Afterwards, calibration standards were processed and assayed as described above, and 10 γL aliquot of supernatant was injected into HPLC for analysis. The calibration curves in the range of 0.1095~10.95 μg/mL for FA were constructed by plotting the ratio of peak area between FA and IS against the FA standard concentrations using least-square method.
Three concentrations (high, medium, and low) of standard solution mixture in the blank rat plasma were detected five times on the same day for intra-day and continuously for five days for the inter-day accuracy variation test. The precision coefficient of variation (RSD) was calculated from the observed concentration (
The pharmacokinetic data analysis was processed by a pharmacokinetic software package of DAS 2.1 which is edited by the Mathematics Pharmacological Committee, Chinese Pharmacological Society. The values of the experimental data and the pharmacokinetic parameters were expressed as mean γ S.D. The following pharmacokinetic parameters such as elimination half-life (
This study suggested that the pharmacokinetic process of herbs will alter in pathological conditions, and can be influenced by other herbs when they are used as a herb pair or formulae. The knowledge of the pharmacokinetic processes of herb pair or formulae in normal and pathological conditions can help us explain and predict a variety of events related to the efficacy and toxicity of TCM.
This research was financially supported by National Natural Science Foundation of China (30873235), National Key Technology R&D Program (2008BAI51B01), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20113237110010), 2009' Program for New Century Excellent Talents by the Ministry of Education (NCET-09-0163), Natural Science Foundation of Jiangsu Province, China (BK2008455), Key Research Project in Basic Science of Jiangsu College and University (06KJA36022, 07KJA36024, 10KJA360039), 2009' Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education, Fundamental Research and Important Incubation Project by Nanjing University of Chinese Medicine (08XPY03). This research was also financially supported by Construction Project for Jiangsu Engineering Center of Innovative Drug from Blood-conditioning TCM Formulae, and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (ysxk-2010). We are also pleased to thank Waters China Ltd. for HPLC technical support and Jinchun Zhang (Nanjing Hospital of TCM) for the periphery blood index test.
Chemical structures of: (
Representative chromatograms of blank plasma sample (
Plasma concentration-time curves of FA in normal (N) and blood deficiency rats (M) after oral administration of RAS, RLC and RAS-RLC extract (at a dose of 6.48, 7.19, 7.35 mg/kg FA). Each point and bar represents the mean ± S.D. (
The periphery blood index in normal and blood deficiency rats (χ̄ ±
|14.78 ± 3.31
|7.01 ± 1.59
|140.87 ± 10.22
|0.42 ± 0.04
|133.25 ± 10.04 **
|3.72 ± 0.88 **
|92.33 ± 5.28 **
|0.33 ± 0.05 **
Compared with normal group: **
Pharmacokinetic parameters of FA in normal and blood deficiency rats plasma after oral administration of RAS, RLC and RAS-RLC extract (at a dose of 6.48, 7.19, 7.35 mg/kg FA).
|Blood deficiency
|3.902 ± 0.783
|28.797 ± 5.208
|5.342 ± 0.802
|31.231 ± 3.493
|28.984 ± 3.066 #
|45.785 ± 6.224
|53.125 ± 7.663
|37.126 ± 2.545
|21.787 ± 2.693
|23.121 ± 4.005
|30.117 ± 2.056
|7.965 ± 1.002
|23.005 ± 3.031
|4.318 ± 0.228
|9.633 ± 2.209
|14.972 ± 0.206 #
|0.886 ± 0.237
|0.905 ± 0.218
|0.760 ± 0.186
|0.732 ± 0.056
|0.327 ± 0.093
|0.114 ± 0.002 *
|21.649 ± 5.292
|16.925 ± 3.368
|20.194 ± 5.000
|38.504 ± 4.912
|88.685 ± 5.589
|118.336 ± 5.876 * ##
|25.689 ± 2.664
|29.013 ± 2.276
|27.412 ± 3.638
|51.487 ± 4.240
|94.457 ± 5.898
|175.970 ± 3.437 ##
|0.759 ± 0.018
|0.035 ± 0.015
|0.309 ± 0.043
|0.077 ± 0.000
|0.008 ± 0.000
|0.615 ± 0.187
|0.043 ± 0.003
|0.318 ± 0.059
|0.030 ± 0.000
|0.013 ± 0.001
|0.040 ± 0.015
|0.022 ± 0.001
|0.013 ± 0.003
|0.070 ± 0.024
|0.012 ± 0.000
|0.014 ± 0.001
|9.898 ± 1.025
|5.000 ± 0.000
|8.775 ± 1.202
|9.996 ± 0.889
|16.667 ± 2.583
|15.000 ± 0.010
|0.444 ± 0.112
|0.321 ± 0.026
|0.582 ± 0.124
|0.918 ± 0.320
|1.962 ± 0.042
|1.088 ± 0.202 *
Blood deficiency groups compared with their normal groups, respectively (*


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About the Book
Photos taken on a visit to Kensington Mine in Alaska, under freezing conditions.
Features & Details
- Category Travel
Standard Landscape, 10×8 in, 25×20 cm
58 Pages
- Publish Date Jan 01, 2011
- Language English
- Tags Blue Sky Mine (song), Coeur Alaska, Midnight Oil, Kensington Mine, Juneau, Alaska, mining, winter
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About the Creator
Marthe Bijman
Vancouver, Canada
Marthe Bijman designs books with her own photos as well as the excellent photos taken by Mike O'Brien, a Geologist, on his travels around the world.
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nursing aidnoun [ C ]uk /ˈnɜː.sɪŋ ˌeɪd/ us /ˈnɝː.sɪŋ ˌeɪd/ Australian English
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Word of the Day
a feeling of respect or admiration for someone or something


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St. Clair Shores
Lakeview softball team earns third district title in four years
Other local teams exit playoffs
By Jason Carmel Davis
Posted June 12, 2013
St. Clair Shores Lakeview softball coach Becky Quinn left the program for a year after relocating for a job.
In Quinn's first year back — and her third overall — the Huskies' coach has guided the team to its third district title in four years.
Lakeview (9-12 at press time) knocked off Grosse Pointe North 8-5 in a Division 1 district final played June 1 at Grosse Pointe North. The Huskies beat East Detroit High 18-0 a district semifinal played June 1 to advance to the final.
Lakeview pitcher Alexis Nowowiecki earned both wins for the Huskies.
"It's pretty exciting. I knew we had a good group of girls coming into the year," said Quinn, whose team went 5-7 in the Macomb Area Conference White Division. "I was excited to get the team back and get things rolling."
Lakeview's regional semifinal against Garden City High took place after press time. The Huskies' district title made Lakeview back-to-back district champs. Lakeview also won a district championship in 2010. In 2011, the Huskies lost 3-2 in extra innings in a district-title tilt.
"(Winning a district title) gives you confidence," Lakeview junior Becky Corbett said. "We've won district titles (three of the last four years). It's a good feeling that people know we have a good program."
Quinn said she and assistant Jodi Witczak used the regular season to prepare for a playoff run. The Lakeview coach, who played softball at Wayne State University, said she and her team focused on the mental aspect of the game for much of the campaign.
Quinn added that offense has been a focus of the team all season. She noted that defense can get a team stops, but scoring runs wins games.
"During every single practice, we talked about that at the end of the season — when it comes down to that final inning — what are you going to do with it?" Quinn said. "This is what we worked all season for. This is when things really count."
This point in the season has seen the intensity ramped up at Lakeview practices, Corbett said.
"We've been a little more pumped up in getting ready for the playoffs," Huskies sophomore Taylor Genzman said.
Genzman and Corbett are a part of a large group who should return to the team next year. Lakeview loses just three players to graduation, according to Quinn, who added that the team could make another run next year.
"We do lose three starters, but we start four juniors who should all be back, and we've got a lot of good girls coming in to fill certain roles," Quinn said. "So I think we'll be solid going into next year, too."
Other local teams bow out in district round
The Lakeview baseball team fell 11-1 to Grosse Pointe South in a D-1 district final played June 1 at Grosse Pointe North. The Huskies, who end the season 5-18, knocked off East Detroit 13-3 to advance to the district final.
The Lake Shore baseball team (13-13) saw its season end in a 10-6 loss to Warren Woods Tower in a D-1 district semi played June 1 at Tower. Lake Shore's softball team (21-11) fell 4-3 to Harrison Township L'Anse Creuse in a D-1 district final played June 1 at Tower. The Shorians' softball team knocked off Tower 7-2 to advance to the final.
South Lake (11-11) baseball lost 10-0 to Center Line in a D-2 district final. The Cavaliers beat Warren Lincoln 5-4 to advance to the final. South Lake's softball team (18-2) also fell to Center Line by a score of 12-2 in a D-2 district final. The Cavs bounced Lincoln 6-1 to advance to the final.
About the author
Staff Writer Jason Carmel Davis covers prep sports throughout Macomb County. Jason has worked for C & G Newspapers since 2011 and graduated from Michigan State University.
More from C & G Newspapers
Dakota softball rallies into the semifinals
Macomb Township
Liggett softball season in the state playoffs
Grosse Pointes
Dakota ace earns top recognition
Macomb Township
Softball All-Star games back for 36th year
Metro Detroit
Dakota softball finds redemption in state final
Macomb Township


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break down
— phrasal verb with break uk /breɪk/ us /breɪk/ verb broke, broken
B2 If a machine or vehicle breaks down, it stops working:
More examples
- The school bus broke down and had to be towed to the nearest garage.
- Oh no - has your washing machine broken down again?
- I'm worried that his car has broken down, or worse still, that he's had an accident.
- If the central heating breaks down again, I will refuse to pay the repair bill.
- Our dishwasher broke down just a month after the guarantee had expired.
Thesaurus: synonyms and related words
› If a system, relationship, or discussion breaks down, it fails because there is a problem or disagreement.
Thesaurus: synonyms and related words
- abandon
- all good things (must) come to an end idiom
- and have done with it idiom
- be over the hump idiom
- bitter
- cure
- knock
- knock off (sth)
- lay
- lay sth to rest idiom
- lay the ghost of sth (to rest) idiom
- leave it at that idiom
- lid
- lift
- snap
- suppress
- suppression
- the curtain falls on sth idiom
- to the bitter end idiom
- top sth off
Thesaurus: synonyms and related words


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Platinium mine after platinum mine, the labour unrest in South Africa is spreading.
Terence Goodlace, chief executive at Impala, the world's second-biggest platinum miner, warned on Thursday of a "significant risk to the industry."
The warning came only a day after workers at two other platinum mines also demanded higher salaries and blocked the entrance to several underground pits, prompting concerns of full-blown industrial unrest in South Africa, one of the world's most important commodity producers. The country is home not just to platinum but also to significant deposits of dozens of other minerals.
Yet, industry executives, analysts and bankers point out that the unrest so far remains concentrated in the restive platinum sector, with little sign that it is spreading to other commodities such as gold and iron ore, which have a history of better labour relations.
The violence – which has left at least 44 people dead and more than 70 injured – started two weeks ago when a group of drill operators at London-listed Lonmin demanded substantial wage increases amid a turf war between rival unions.
Platinum prices surged to a four-month high of $1,558.49 (U.S.) per troy ounce on Thursday, extending a rally to 12 per cent over the past week, as nearly a fifth of the world's output was shut down to mark a memorial for the victims.
Peter Attard Montalto, analyst at Nomura, warned that the crisis at Lonmin was "only the tip of the iceberg," adding that if the miner caves in and meets its workers' wage demands, then demands by other workers "will increase across the mining sector and not just in platinum." Some other executives share this concern.
Yet, the price of other metals and minerals for which South Africa is a key producer remains largely unaffected. The chamber of mines of South Africa says 95 per cent of the country's mines are operating as normal.
But the central role that the country plays in the global natural resources market is keeping everyone on edge. South Africa holds the world's largest reserves of more than a dozen minerals, including platinum, manganese, chromium, gold and aluminum silicate. On top of that, the country holds also considerable deposits of other commodities such as vanadium, zirconium, titanium, antimony, nickel, uranium, thermal coal and iron ore.
But the platinum industry differs in key respects from the rest of the mining sector, analysts say, and it is for these reasons that the unrest has so far been limited to that sector.
First, platinum is particularly labour-intensive and dangerous, with drillers working in difficult conditions in some of the deepest mines in the world. Ivan Glasenberg, the South African chief executive of Glencore, the commodities trader, notes that other mining sectors, such as coal, are generally "more mechanized." Moreover, iron ore and coal are extracted largely from safer open-pit mines.
Second, wage negotiations in the South African platinum industry are largely conducted company by company, rather than under the umbrella of an industry association. This gives extra power to unions and can result in violent strikes.
Third, the combative Association of Mineworkers and Construction Union – a rival to the traditional long-dominant National Union of Mineworkers – has made large inroads among disaffected platinum workers, prompting more extreme demands. Nick Holland, chief executive of Gold Fields, says AMCU, the splinter union behind the original strike by rock drill operators at Lonmin's mine, has not made noticeable inroads in winning members at its gold mines.
"One of the benefits in the gold industry … is that we have central bargaining agreements," says Mr. Holland.
Finally, workers in other sectors, such as gold or ferrochrome, tend to live on site, with accommodation, medical care and housing facilities provided for them by mining companies. In contrast, in the platinum sector, workers are often bussed in from nearby villages, where living conditions are quite often precarious.
But ultimately, executives agree that these differences could mean little. Further violence could trigger a domino effect, hitting all of South Africa's mines.
Additional reporting by Helen Thomas in London.Report Typo/Error


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Heidi Montag Is "Superficial"
Yeah, we already knew that, but now the Hills villainess has officially admitted to her shallow nature in song.
Heidi Montag has unleashed her latest single, "Superficial," for the waiting ears of the world, and it's quite the revelatory little number. On the track, Montag details her fabulous life of Maseratis and paparazzi, declaring,
"They say I'm superficial, some call me a bitch/They're just mad, 'cause I'm sexy famous and I'm rich."
All too true, Heidi, but can you blame them? After all, there's just so much to envy about you.
Check out the tune and share your critiques in the comments section!


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My mother is taking Zyprexa at night and Paxil during the day. Is anyone aware of whether Zyprexa causes hallucations? She is seeing things that are not there, but she feels they are real.
yes it can cause hallucinations along with many other side effects. some are alterted mental status, seizures, insomnia, anxiety, personality disorder, amnesia, obsessive compulsive disorder, phobias,hang over effect, and twitching. There are at least 100 others.
Forum Rules


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September Tidbits: Openings, reopenings & comfort food
All summer long I've been collecting these tidbits, who is opening, or reopening, or expanding, or even, closing. It's mostly good news.
Kim Quay opened Comfortfood, a cozy storefront take-out and catering business, in Morrisville eight years ago. It's one of those best-kept secrets. I'm not sure the folks in Lower Bucks really want the […]
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June 2015 Tidbits: Restaurants and markets abound!
From Doylestown to Silverdale to New Hope and beyond, there are so many food happenings in Bucks and nearby this month.
One of our favorite stops on the Meat Alley is Bolton's in Silverdale, right on Route 113. Although they are best known for raising all natural turkeys year round (no […]
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On the local side: Where we ate in 2013
Contrary to popular belief, we do not eat out every night. Neither my bank account nor my waistline can afford it. That said, when I look back on 2013, we've eaten well. Here's a review of some of the places Bucks County Taste has dined.*
Local, local, local. This has been a great year […]
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Announcing a new Meetup for Make:SF - the Bay Makers!

What: Make:SF Nov 2nd @ Tech Liminal

When: Tuesday, November 2, 2010 7:00 PM

Where: TechLiminal
268 14th Street
Oakland, CA 94612
510-832-3401

Hello Makers,

First of all, thanks to all who came to the East Bay Maker Faire. It was a great success despite the rain. We had a constant stream of kids coming to check out and make TV-B-Gones and we can't wait for next year.

This month Ponoko is back with a combined speaker/miniproject extravaganza at a brand new time of 7:00pm! They be taking us through a miniproject that will show how to get a design ready for laser cutting. The project is free but you will need your computer with a copy of Inkscape installed on it and you will need to download these templates as well. For those of you who do not have a computer we will have a few that you can use on hand.

And of course we want to see your projects. So bring them in and show them off!

This fun-for-all-ages event starts at 7:00pm. Children under 14 need to be accompanied by an adult.

New to Make:SF? Here is what you can do:
 - New to making? Come and explore with a fun mini-project.
- Have an idea? Come and we will help you get started making it.
- Making something? Come and demo it. Get feedback and network with the Makers.
- Come to see the speakers we bring in talk about their projects, tools you can use, etc.

RSVP to this Meetup:
http://www.meetup.com/makesf/calendar/15256774/

 --
 Please Note: If you hit "REPLY", your message will be sent to everyone on this mailing list (email@example.com)
 This message was sent by Malcolm Knapp (email@example.com) from Make:SF - the Bay Makers.
 To learn more about Malcolm Knapp, visit his/her member profile

                  Meetup, PO Box 4668 #37895 New York, New York 10163-4668 | firstname.lastname@example.com


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Search Results for "vPath"
Nexus 1000V Expands Cloud Network Services Ecosystem with vPath Integration in the Imperva Web Application Firewall
…sed in a ordered chain defined by policy. Last year, Imperva announced integration with the Nexus 1110 Cloud Services Platform. This latest demonstration shows further simplification through interactions with theRead More
Unified Data Center IQ Contest : And if there is only ONE ..
…g languages supported by onePK? C and Java 2. What is the name of the OpenStack API that deals with L2 networking? Quantum 3. What is the name of theRead More
New Nexus 1000V Free-mium Pricing Model
…customers to date. The Nexus 1000V Essential Edition provides all the rich Layer-2 networking features to connect virtual applications to the network and integrate into VMware environments, including: VXLAN capability,Read More
Nexus 1000V Provides Cloud Switching to New Windows Server 2012 Platform
…NAM), and manageability through our Data Center Network Manager (DCNM) to Hyper-V environments (Click here to view the on-stage demo – Nexus 1000V demo starts at 37:20). VSG and otherRead More
ASA 1000V Cloud Firewall Now Available to Secure Multi-tenant Data Centers
…and DHCP. This will complement our Virtual Security Gateway (VSG) firewall which has greater visibility to VM-specific policy attributes, and will be used to isolate VM-VM traffic within a tenant.Read More
The Next Evolution of Cisco's Nexus 1000V Virtual Switch to be Featured at VMworld
…Firewall, VSG zone-based virtual firewall, vWAAS WAN optimization, the Cloud Services Router (CSR) 1000V, and Cisco Prime Network Analysis Module (NAM) and built on Cisco's advanced service insertion and chainingRead More


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You are reading the older HTML site
Positive Feedback ISSUE11
january/february 2004
Vaughn Williams: Fantasia on a Theme of Thomas Tallis; Norfolk Rhapsody No.1; In
the Fen Country; Fantasia on Greensleeves; Concerto grosso
James Judd refreshes these Vaughan Williams chestnuts with a distinctly American directness of address and clarity of line, without violating their essential songful breadth.
The introductory chords of the Tallis Fantasia, for example, usually hang in the air ambiguously, as if suspending time; Judd paces them more tightly, shaping them into a clearly directed phrase from the outset. Shortly thereafter, the main theme sings with its customary grave dignity, but its underlying buoyancy has almost a waltz feel, and Judd propels the central section of the piece urgently and dramatically. Indeed, the conductor tends actively to impel the climaxes forward, rather than "allowing" them to unfold in the manner of the best British practitioners; but the music never feels pressured - there's always space and time to breathe. The viola solo midway through sounds unsettled, but the violin solo later on pulses with vibrant life.
Similarly, after the fragile hush of its opening, the shapely lyrical lines of the Norfolk Rhapsody No.1 - there never was a No. 2 - fill out the textures richly. And the strings lean into Greensleeves's simple modal melody so as to underline its ambivalent emotional undercurrents; the cool, clear flute solos bewitch the ear.
The Concerto grosso for strings is less of a chestnut, and collectors will enjoy hearing it played by a full string orchestra instead of the usual chamber-sized group. The firm, full-bodied tone and attack is gratifying, especially effective in bringing out the central Sarabande's dusky solemnity, while the tightly focused bass reproduction provides a solid foundation to the sonority.
Only In the Fen Country - the sort of English pastoral that reduces Vaughan Williams to a sort of extra-good Delius - rambles on too long, as it usually does. Even here, the orchestra sounds unfailingly beautiful; the solo horn phrases are especially plangent.
No real complaints about the full, clear engineering, although I expected the acoustic to blossom more in peak passages - those of the Tallis Fantasia seem a bit dry and unblended. Warmly recommended nonetheless.
Zemlinsky: Symphonies: No.1 in D minor*; No.2 in B flat+
The "Mahler connection" has raised the profile of once-neglected Viennese composer Alexander von Zemlinsky (1871-1942). He was a conducting colleague of Gustav's, and Alma's composition teacher; Arnold Schoenberg was his brother-in-law. But you won't hear these composers in these early symphonies, whose firmly traditional, post-Brahmsian idiom and rhetorical gestures contrast markedly with the Expressionism of Eine florentinische Tragödie and Zemlinsky's other operas. Only the Second Symphony's Adagio evinces some "advanced," emotionally ambiguous harmonies. Mendelssohn's graceful treble chatter is a clear influence, while Zemlinsky indulges a penchant for Wagnerian chorales as well.
The new Naxos disc recouples performances originally issued on full-priced Marco Polo in the Eighties. The three-movement First Symphony comes across well enough at Ludovit Rajter's hands: greater drive would not be amiss in the rather careful, deliberate first movement, but the central Scherzo is pointed and graceful, and once the finale gets going, it develops a nice airborne buoyancy. The more ambitious Second Symphony makes a stronger mark - the composer infuses the chorales' fervent grandeur with a softening lyricism, and a really sharp, dramatic conflict develops in the finale - and Edgar Seipenbusch does well by it. Both orchestras fill out the cantabile lines with warm, vibrant string tone; the Slovak Philharmonic in the Second boasts full-throated brass, though they "stick" rhythmically against the tremolo strings on several occasions, and the last tempo change in the first movement takes a few bars to settle. The full, vivid sound has the mild reverberant overhang typical of the initial run of Marco Polo productions. Definitely worth getting to know.
Stephen Francis Vasta


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Hey everyone
So my battery has been evaluated by the iStore as healthy, and yet it is swelling quite badly. MacBook does not sit level, and trackpad is becoming useless.
iStore rep told me I'm looking at R4-6k for a new battery, and 7-10 days.
I'm tempted to buy my own battery via Amazon and do this myself, because at that price I much rather invest in a new MacBook.
Does anyone have any experience with changing their own batteries, or perhaps an alternative I can pursue?
I have a late-2013 maxed out Retina MBP. I love her dearly.


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When Rykarda Parasol took the stage at the Jewish Culture Festival on July 6 and 7 in Krakow, Poland, the singer wowed the crowd with her moody pop style ("Noir rock," she calls it).
Her appearance especially touched one special guest in the crowd: Parasol's father, Polish-born Jew Richard Parasol, who decades earlier survived the war because a brave Christian family hid him.
It was a poignant moment for the singer.
Said Parasol in a phone interview from Israel, "For a father who was against my being in music, he was able to see that while art and entertainment are somewhat of a luxury, art really does connect all of us."
Born in San Francisco and raised by her Polish-Israeli father and Swedish mother, Parasol embodies several conflicting traits — from tolerant Bay Area universalist to Parisian café-society girl.
There's another one near the surface: daughter of a Holocaust survivor. That tragedy colored her upbringing and influences her art today.
"He was open to talking about [the Holocaust]," Parasol recalled of her upbringing. "I did some research on children of Holocaust survivors, and there are some similar traits between them and me: separation anxiety, feeling like you want to take on their pain."
It was a lot of pain. After his family was forced into the Czestochowa ghetto and killed, Parasol's father made it through the ghetto walls, taken in by a Catholic family that knew him. He was 6.
After the war, he made his way to Palestine, where he built a new life, serving in the army and becoming a proud Israeli. Years later, while studying engineering in San Francisco, he met his Swedish wife-to-be.
Remembered Parasol, "He told me had he met this big blond and he said if he were to have a child with this woman it would be Hitler's worst nightmare."
Fast-forward, and their artistically restless daughter wandered away from the Bay Area to Los Angeles, Texas and France, polishing her craft as a songwriter and performer.
The smoky-voiced singer has recorded two albums, with a third due this fall. She's played South by Southwest in Austin and the Hardly Strictly Bluegrass festival in San Francisco.
But Parasol spends most of her time in Paris and Poland, drawn there by the arts scene. She has a band in Warsaw, which backed her on stage in Krakow.
The irony of finding success in the same country where her forebears were murdered is not lost on her.
"There's this strange irony," she said. "Isn't it great that someone can feel safe enough today to stand on stage as a Jew and for him to see it?"
She admits she feels a lingering undercurrent of anti-Semitism in Poland. During one concert there, a youth started giving her the "Heil Hitler" salute, and some of her Polish friends have advised her not to reveal her Jewish identity because she might "run into problems."
"On the other hand," she said, "I've been to a few Eastern European countries, and Poland is so progressive, with a stable economy, extremely current and with it."
So for now, she's sticking with Paris, where she spends most of her time, though she will be back in the Bay Area for spell later this year.
"I live inside the Hebrew 'hood," she said of the Marais, Paris' traditional Jewish area. "It's mostly Ashkenazi bakeries and delicatessens. It's nice for me to buy bread on Friday and say 'Shabbat shalom.' It makes me feel like I'm not such a stranger in a strange land."


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In the early 17th century, slaves brought sweet sorghum seeds, then known as "Guinea corn," to the United States.
In the West Indies maize, Guinea corn, sugar-corn tops, and sometimes molasses are given.
And Guinea corn, which thrives well here, serves for the same use.
It is a fermented drink made from Guinea corn, and is, I was told, highly intoxicating if one drinks enough of it.


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How could I center multiple images on the same 'line'?
Hi all,
I find the image alignment defaults to be very limiting. At present I can align left, right, center or none. If I align center then I can have one image per 'line', but if I were to put two images in the edit field, both aligned center with no line breaks between them, I'll still get one image aligned center, then another below it also aligned center.
This is a waste of space – what I would like to be able to do is put two or even three images all next to each other, but have the unit that is made up of the three of them aligned center so that there is equal white space to the left of the leftmost image and right of the rightmost image.
I'm guessing that the only way to do this is to align them all to 'none', place them next to each other in the Edit Post markup, then wrap them in some inline styling code right there in the Edit Post box that aligns the whole lot center, but I can't work out how to achieve this.
In the long term I'd prefer it if I could do some CSS coding that recognises when multiple images are placed together and aligned center, and realise that I don't want them all on separate lines.
If anyone could show me what I'd need to do to achieve either or both of these potential solutions I'd be really grateful!
- The topic 'How could I center multiple images on the same 'line'?' is closed to new replies.


text:
Lost my motivation and my way, need to start from the beginning
View Single Post
Senior Member
Join Date: Feb 2007
Posts: 1,290
/ 0%
Originally Posted by
Hey man, it is very usable.
Offline marketing is blowing the fuck up, I only have TWO clients, earning me $5000 a month! My city only has a population of like 60 thousand, and theres maybe 5000 businesses in my city. I could get 100 paying me $1000 a month earning $100,000 a month but that is too much work for me. I'm lazy.
There is so much resources for it, it's really easy to get into, you just need to take action immediately.
Offline Marketing Discussions
Yeah I was about halfway thru the first video when I found the thread mentioned in the demonoid comments. A lot of people were saying he disappeared halfway thru it and didn't give all the information.
Did you really have to go thru all the stuff he's saying, creating a site, and an ebook, and a mp3, and etc etc or not really?
Was just making sure there was enough information in there to make use of it because the first video is like 2 hours long.
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TRANSFER-listed striker Ashley Grimes scored twice as Bury kicked off their pre-season campaign with a 4-1 win at non-league neighbours Radcliffe Borough.
The 27-year-old former Rochdale forward's brace was bookended by a peach of a strike from James Burke and a fine solo effort from Australian triallist Anthony Laus. Grimes played a more withdrawn role in a three-man midfield that included Burke and Rob Bourne.
It was Burke, signed from Huddersfield as a defender in January, who gave Bury the lead midway through the first half.
And that is how it stayed up to half-time, thanks in part to a smart save from Luke Simpson, who is on trial from Oldham after starting his career at the Shakers Academy.
Grimes, who failed to score for the first team in his debut season at the club after moving from Spotland last summer, bagged a quick-fire double within 10 minutes of the restart.
The first came via a cut-back from triallist Joe Widdowson – a left-back who played under Bury boss David Flitcroft at Rochdale – while the second followed an assist from youth-team striker Anthony Dudley.
Bizarrley, Dudley was then substituted for physio Tom Walsh, before Boro hit the post and then pulled one back through Michael McGinlay - son of former Bury and Bolton Wanderers' striker John McGinlay.
But Bury still had time to add a fourth following a mazy run by ex-Sunderland forward Laus, who dribbled past three Boro defenders before slotting home.
Laus's promising virtuoso display was one of a number of positives to take from the game, despite the lack of first-teamers on display.
Other than Burke and Grimes, Bury's only other senior representatives were at centre-back, where Nathan Cameron partnered new signing Keil O'Brien - who picked up a knock and was substituted after half-an-hour.
Youth players Scott Burgess and Marcus Poshca, who featured in Bury's final match of last season at Morecambe, were also included in the squad.


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Verizon Wireless will begin selling Samsung's competitor to the Apple iPad, the Galaxy Tab, on Nov. 11, the two companies have announced.
The tablet, which will run Google's Android operating software, will sell for $600.
The Galaxy Tab will feature a 7-inch touch-screen, support for Adobe's Flash Player, 3G and Wi-Fi connectivity and access to Google's Android app market. Network plans from Verizon start at $20 a month for 1 GB.
The Galaxy will also include 16 GB of storage with a microSD card slot to add an additional 16 GB.
Last week, Verizon announced it will start selling the Apple iPad on Oct. 28.
Readers, what do you think of the Galaxy Tab?
By Brett Molina
Technology Live covers computers, electronics, and the Internet, with a focus on cutting-edge technology that has a real impact on your life. Our reporters and editors -- based in San Francisco, Los Angeles, Seattle, New York, and Washington – offer news and tips to help you make the most of your digital domain. More about the team
Sign up for Technology Live e-mail alerts


text:
kseniya B Model
Sportiello PhotographyPhotographer Female Kingston, New York, US
My MM URL: http://www.modelmayhem.com/sportiellophotography
Mayhem # 2181943
About MeWelcome MMers! Thank you for the inspiration. I can't believe the extraordinary photography on this site.
Currently, I am searching for female models with unique features to help create beautiful, story-telling, creative, and edgier images. My new focus is edgier...sexier...more original, and in the end, it's about creating art. I want to create something beautiful and unique. I am not interested in taking boring shots of models standing and looking pretty. I want more intensity and emotion.
I am an easygoing female photographer and take pride in taking care of models who join me. I also take into consideration what my models would like to create for themselves approaching it as a team effort. You will find I am silly and comfortable to be around. I take it seriously but love to have fun with it as you can see and I don't care for the cocky. Hoping to meet some great people in the industry. My philosophy is every experience is an opportunity to document life, love, and beauty.
Have you worked with Sportiello Photography? Add Credits for Sportiello Photography! >>
kseniya B Model
lena mae Model
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Sophia May Model
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Colin Davies, 44, was arrested after he was involved in scuffles with officers in the doorway of the Dutch Experience cafe, in Stockport, Greater Manchester.
Davies, who has smoked the drug to relieve pain since breaking his spine four years ago, said before his arrest that he wanted to offer the drug to sick people to help relieve their symptoms. 'He's a healer, not a dealer,' shouted one of his supporters as Davies was put into the back of a police van.
A police spokesman said: 'A 44-year-old man from Stockport was arrested on suspicion of possession of cannabis with intent to supply. A further man, along with four Dutch people, three men and a woman, were arrested on suspicion of being concerned with the supply of a Class B drug. All six are in custody, and the shop is now closed."
Just one uniformed officer was outside the cafe at 9.30am, half an hour before it was due to open. But there were undercover detectives in the area, and Davies was involved in the scuffle as he tried to push a wheelchair user into the cafe. Other people in wheelchairs openly smoked cannabis as police watched.
Among his supporters was Kate Bradley, a former police officer in the West Midlands force, who has smoked cannabis since she was diagnosed with multiple sclerosis in 1991.
Mrs Bradley, who is in her fifties and from Telford, Shropshire, was pushed out of the cafe in her wheelchair by a friend after police entered the shop. She said it had been a frightening experience to be inside when the police entered. Davies's father, Colin, 77, said he supported his son: 'I have seen the benefit cannabis has had on Colin.'
Campaigners continued to protest outside the cafe, as the ribbon which had meant to be cut for its opening lay in tatters on the floor. Inside the shop, decorated throughout in red, were empty tables and chairs, each with an ashtray. Cigarette papers were scattered about.
Steve Taylor, a GP based in nearby Prestwich, said: 'As a doctor, I see the harm that cannabis does to people.'


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This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Although some strains of
Some bacteria serve as distinct nucleation sites for mineral authigenesis
Strains of this genus have been successfully isolated and allocated to their taxonomic positions [
Using this system, the initial phases from the secretion of bacterial materials to the assembly of the immature sheath skeleton and the subsequent deposition of aqueous-phase inorganic elements were examined in OUMS1 grown in natural groundwater conditions. This study focused on structural and compositional differences and similarities between OUMS1 grown in laboratory and natural conditions. The techniques applied in this study included specific staining by light microscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM), STEM-secondary electron imaging (SEI), high-angle annular dark field (HAADF)-STEM, and energy dispersive X-ray (EDX) spectroscopy.
A small bag made of a dialysis tube containing a small aliquot of OUMS1 suspension was found to swell soon after immersion in a container filled with groundwater (
Dialysis tube bags and light micrographs of growing OUMS1 cells and their associated sheaths in groundwater; (
Since this paper focuses on the morphological differences between OUMS1 cells and their associated sheaths formed in medium cultures [
SEM images of a sheath of medium cultures (a) or groundwater (b) at day 14. (
Immediately after harvesting, the medium- and groundwater-sheaths were air-dried on a copper grid covered with a supporting lamella before STEM-SEI and TEM observations. STEM-SEI is formed by a secondary electron beam derived from the specimen surface, whereas TEM images are derived from an electron beam transmitted through the specimen. Therefore, the TEM image is a "shadowgraph" where a transmittable electron builds up a bright image. On comparing medium- and groundwater-sheaths, we observed that the former were almost twice as thick as the latter (
STEM-SEI and TEM images of sheaths of medium cultures (a,b) and groundwater cultures (c,d) at day 14. (
Similar to the medium-cells after 1 day of culture [
TEM images of U/Pb-stained sections of bacterial cells associated with the immature sheath incubated in the groundwater for 1–3 days. (
HAADF-STEM imaging and elemental mapping of groundwater-cells/sheaths in combination with a specific carbohydrate stain (alkaline Bi stain) [
The present dialysis tube method was quite useful for analyzing the reproduction of an isolated strain of aquatic microbes, their mode of production, and the characteristics of their associated structures. This system will be used to analyze the behavior of aqueous-phase metal ions with respect to sheath maturation in medium and groundwater conditions.
OUMS1 (NITE BP-860) in silicon-iron-glucose-peptone liquid medium lacking FeSO4 (modified SIGP [
To grow OUMS1 in natural conditions, a novel dialysis method was devised as shown in
The specimens cultured above were observed by differential interference contrast (DIC) microscopy prior to preparation for electron microscopy. The viability of OUMS1 cells in natural conditions was examined by the dilution plate method as follows. The final bacterial suspension described above was serially diluted and spotted onto modified SIGP agar plates. After 3 days of incubation, colonies on the plates were counted with a stereo microscope (day 0 incubation sample). The same protocol to determine the cell numbers was applied to the specimen removed from a dialysis-tube bag incubated in groundwater for 3 days (day 3 incubation sample).
Specimens from the bag were also subjected to a viability test using a bacterial viability kit (LIVE/DEAD®
The collected samples were washed repeatedly with sterilized ultrapure water and vacuum dried. The dried samples were mounted onto an aluminum specimen stub and vacuum dried for 30 min. After platinum (Pt)-coating, each specimen was observed by SEM (S-4300, Hitachi, Tokyo, Japan). The samples cultured in the modified SIGP liquid medium were treated similarly and observed arbitrarily for reference.
Specimens were collected by centrifugation and fixed with a mixture of 2.5% glutaraldehyde, 1% OsO4, and 4.5% sucrose in 100 mM cacodylate buffer (pH 7.0) on ice for 2 h and then embedded in 3% agar. Small pieces of the washed agar block were dehydrated in a graded series of ethanol and again embedded in Quetol 651 resin mixture (Nisshin EM, Tokyo, Japan). Ultrathin sections were stained with uranyl acetate and lead solution (U/Pb-stained sections) and then observed using a TEM (H-7500, Hitachi, Tokyo, Japan) operated at an accelerating voltage of 80 kV.
Following the carbohydrate-staining technique devised by Park
The Bi- and U/Pb-stained ultrathin sections on the copper grids were first covered with a formvar film using a routine method and then coated with C to increase the tolerance of the specimen to an intense electron beam. HAADF-STEM and EDX detectors attached to a JEOL JEM-2100F (accelerating voltage, 200 kV) TEM were used for HAADF-STEM imaging and mapping of Bi and Fe, respectively. Bright field STEM images were also obtained for reference.
The harvested sheaths from the medium and groundwater cultures were washed repeatedly in distilled water and a suspension was dropped onto a copper grid covered with a C-coated porous thin membrane (NEM, Tokyo, Japan), then air-dried. The specimens were observed by STEM-SEI and TEM.
In this study, an isolated strain of
This study was financially supported by a Special Grant for Education and Research (2008-2013), a Grant-in-Aid for Research Activity Start-up (No. 22860040, 2010) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (for J.T.), and a Grant-in-Aid from the Yakumo Foundation for Environmental Science (for T.S.).