text:
- Take the Blood Disorders Quiz
- Patient Comments: Alpha Thalassemia - Symptoms
- Patient Comments: Alpha Thalassemia - Diagnosis
- Find a local Hematologist in your town
What Genes Are Related to Alpha Thalassemia
Alpha thalassemia typically results from deletions involving the HBA1 and HBA2 genes. Both of these genes provide instructions for making a protein called alpha-globin, which is a component (subunit) of hemoglobin.
People have two copies of the HBA1 gene and two copies of the HBA2 gene in each cell. Each copy is called an allele. For each gene, one allele is inherited from the father, and the other is inherited from the mother. As a result, there are four alleles that produce alpha-globin. The different types of alpha thalassemia result from the loss of some or all of these alleles.
Hb Bart syndrome, the most severe form of alpha thalassemia, results from the loss of all four alpha-globin alleles. HbH disease is caused by a loss of three of the four alpha-globin alleles. In these two conditions, a shortage of alpha-globin prevents cells from making normal hemoglobin. Instead, cells produce abnormal forms of hemoglobin called hemoglobin Bart (Hb Bart) or hemoglobin H (HbH). These abnormal hemoglobin molecules cannot effectively carry oxygen to the body's tissues. The substitution of Hb Bart or HbH for normal hemoglobin causes anemia and the other serious health problems associated with alpha thalassemia.
Two additional variants of alpha thalassemia are related to a reduced amount of alpha-globin. However, cells still produce some normal hemoglobin, these variants tend to cause few or no health problems, and the loss of two of the four alpha-globin alleles results in alpha thalassemia trait. People with alpha thalassemia trait may have unusually small, pale red blood cells and mild anemia. A loss of one alpha-globin allele is found in alpha thalassemia silent carriers. These individuals typically have no thalassemia-related signs or symptoms.
How Do People Inherit Alpha Thalassemia?
The inheritance of alpha thalassemia is complex. Each person inherits two alpha-globin alleles from each parent. If both parents are missing at least one alpha-globin allele, their children are at risk of having Hb Bart syndrome, HbH disease, or alpha thalassemia trait. The precise risk depends on how many alleles are missing and which combination of the HBA1 and HBA2 genes is affected.
Medically reviewed by Jeffrey A Gordon, MD; American Board of Internal Medicine with subspecialties in Oncology and Hematology
Last update: 8/1/2009
SOURCE: Genetics Home Reference. Alpha Thalassemia.


text:
Romanians are quick to point out that the vampire lore is fiction — torture tactics aside, Vlad "the Impaler" is revered for his efforts to unite Romania. Yet Transylvanian tourist shops happily capitalize on foreigners' misconceptions by hawking Dracula merchandise.
Which I, of course, couldn't resist. Two "I Love Dracula" bumper stickers and a stack of Vlad postcards later, I trekked to the old Saxon cemetery behind Sighisoara's 16th-century Church on the Hill. Ambling between the overgrown plots, I recalled a conversation with a neighbor back home. She told me her husband had Romanian ancestry — her brother-in-law used to tease her kids that they were descendants of Dracula. She laughed and said the kids ought to look at the photos of my trip to see what their ancestors' country was really like.

subset: commoncrawl


text:
December 7, 2006
Dear FOS11 Member,
In the final phase of its response to the events of September 11, 2001, EPA has announced the beginning of a program to test indoor spaces in Lower Manhattan. The program, which covers the area south of Canal Street and west of Allen and Pike Streets, will allow residents and building owners to have the air and dust in their units tested for four contaminants associated with dust from the collapse of the World Trade Center. If analysis of dust and air samples finds elevated levels of any of four contaminants of concern – asbestos, man-made vitreous fibers such as fiberglass, lead, and polycyclic aromatic hydrocarbons – the contaminants will be cleaned up.
Under the program announced today, local residents and owners of commercial or residential buildings closest to Ground Zero will receive priority for testing and cleaning if registration for the program is high. The program is limited to $7 million provided by the Federal Emergency Management Agency. The number of residential and commercials spaces that will be addressed under the program will depend upon a number of factors, including the square footage of the units participating.
Registration for the program will begin mid-January and run for two months.
For more information , visit http://www.epa.gov/wtc/testandclean or contact the Indoor Air Residential Assistance Program at (212) 637-3435.


text:
Thursday, May 21, 2009
Re: Re-querying Redux
When is it okay to re-query the same agent? When is okay to query someone else at the same agency?
Here's a (hopefully) comprehensive list of scenarios (and please bear in mind that these are just my opinions and others may feel differently):
If the agent passed on your query: Do not re-query with the same project, even if you've revised your query and/or manuscript. The agent has made their decision.
If the agent passed on your partial or full: If the agent specifically asked for revisions they are expecting they will hear from you again (and please check out this post for more about those expectations). If they didn't specifically ask for revisions, most agents will be open to hearing from you again about the same project if they provided specific advice and you took said advice. Whether they will ask to see the revised manuscript again is decided on a case by case basis. UPDATE: And as Jessica writes, definitely remind them that they had seen an earlier version.
Querying an agent who previously passed on your work with a new project: If the agent previously passed your query for a previous project, especially if it was a form rejection, I wouldn't mention the previous query. If the agent requested a partial or full but ultimately passed, definitely mention this to them when you query for your new project.
But whatever you do, wait three to six months after receiving a rejection from an agent before querying them with a new project. There's really nothing worse than passing on a project and then getting an immediate e-mail back about a different one. I just passed on one work, am I really going to be predisposed to saying yes to the one that comes five minutes later?
Querying different agents at the same agency with the same project: Do not query two agents at one agency simultaneously. If you receive a rejection from one agent, it is usually okay to query another agent at the same agency, but check their guidelines. If they don't specify otherwise you may query another agent after receiving a rejection from one, but wait at least three months. You never know if agents share assistants, and you don't want them to think you're papering the world with queries.
And whatever you do, do not e-mail an agent back and ask them to refer you to someone else or ask for query tips. If your query wasn't quite right for me but I can think of someone else, I'm going to refer your query anyway. And no agent I know has time for personalized query tips.
Hope this helps! Let me know if I missed anything in the comments section.
104 comments:
- JohnO said...
How bizarre. You and Jessica Faust post on the same topic, on the same day.
- May 21, 2009 at 1:30 PM
- Annalee said...
Are mail management systems used at all in agent-land?
I use one for my work (which is not publishing-related), and it's always been useful when I've got a letter in front of me to be able to see the entire correspondence history with that person, and have nifty statistics like turnaround time and volume of correspondence on particular issues tracked automatically.
No reason for asking; I'm just curious.
- May 21, 2009 at 1:32 PM
- Nathan Bransford said...
I called Jinx on Jessica, so she owes me a coke.
Although since she technically posted first I guess I owe her a coke.
- May 21, 2009 at 1:32 PM
- Justus M. Bowman said...
"If the agent passed on your query: Do not re-query with the same project, even if you've revised your query and/or manuscript."
This one surprised me.
- May 21, 2009 at 1:33 PM
- BookEnds, LLC said...
That's hysterical! Great minds I guess. Great though, different agents, different perspectives.
I'll be happy to buy the coke, maybe a Jack and coke?
- May 21, 2009 at 1:34 PM
- lynnrush said...
Great info, Nathan.
- May 21, 2009 at 1:36 PM
- WindyA said...
Thanks for the advice. Good clarity around what to do with multiple projects and past rejections.
- May 21, 2009 at 1:40 PM
- Mandy said...
I think re-querying after just your query was rejected might be subjective. I had a horrible query letter that was being shot down a lot. Once I restructured the letter and made some major changes, I re-queried some of the agents who had rejected me and received requests for additional material based on my re-query. So I really feel like it depends on the individual agent.
- May 21, 2009 at 1:41 PM
- Laura Martone said...
I appreciate the advice, Nathan - although I agree with Mandy that the "rules" might vary from agent to agent.
On a personal note, the suggested waiting times can sure add up. No wonder my college professors told us writing majors that getting a novel published would be highly unlikely.
But you can't give up, right? The most successful writers I know believe that perseverance and a thick skin will pay off in the end... wish I was a crocodile.
- May 21, 2009 at 1:50 PM
- Nathan Bransford said...
What I will say in the comments section but not the main post is that if you really truly believe in good faith that you have drastically improved your query and would like to try again, they're probably not going to notice that you did so, and unless they personalized their response to you I wouldn't mention that it's a re-query.
However, I beg that people don't abuse this because if everyone kept re-querying my life would be insane.
- May 21, 2009 at 1:54 PM
- pilot said...
Great post Nat. You don't just give some basic rules of approaching with a re…. but also a few suggestions regarding politeness. One question though, you're targeting an agent (because, after research, you feel he's the right one for your work), your query must be at least decent (for you've got a high percentage of requests from others), your writing is more than just adequate (for beta-readers and free-lance editors say they love it)…. So, how do you get by the assistant?
- May 21, 2009 at 1:55 PM
- Luisa Perkins said...
This is so helpful; thanks, as always, for your generosity.
- May 21, 2009 at 1:56 PM
- LorelieLong said...
This is just idle curiosity, because my mind wanders like this, so feel free to ignore if needed. :D
What if you write in two genres, and have a completed project for both. At an agency are two agents, each of whom works in one of your two genres. Could you query each of them with the separate projects at the same time?
- May 21, 2009 at 1:57 PM
- Nathan Bransford said...
You get by the assistant by writing a good query so they'll pass it on to their boss.
I recommend that people focus on querying one project at a time anyway.
- May 21, 2009 at 2:00 PM
- Bane of Anubis said...
I would never think of re-querying for the same project (that got form rejected), but it sounds like some people do that - something I can empathize with b/c most of us are fretting over the effectiveness/quality of our queries.
Personally, it's hard to get a sense of the query. I'll write it, think it's great, then hate it the next day. I'll send one version out to one agent and then a different one to another - if I find success with one, I think "maybe that one's better" and then it won't find traction again - probably b/c as all you agents seem to emphasize, there's a bit of subjectivity involved.
I'd also never considered re-querying for partials/fulls that were ultimately passed up. Gosh, now I might have to go back and look at some old projects to see if they're worth re-visiting.
Thanks for the guidelines (i.e., things we can follow and know we're doing right). The next time I query you, I'm gonna make sure I use block formatting, CAPS, etc...
- May 21, 2009 at 2:02 PM
- Stephanie Faris said...
Thanks for the advice. I know I've annoyed editors at publishing houses probably by firing back another query a month or two after they rejected the last one. I'm not sure how they feel about that but the response time from publishing houses is sometimes ridiculously long so of course I'm going to have something else ready to send them after they've held my other query for six months!
- May 21, 2009 at 2:03 PM
- Heidi said...
Nathan - you mentioned not asking an agent for referrals, which I completely get, but here is my dilemma.
I queried a highly reputable agent who requested my full. She read and responded in a very gracious email that she "was very impressed with this manuscript...the quality of writing is far superior to most of that which crosses my desk," and that she felt very connected to the characters and the story. HOWEVER she didn't feel she had the right contacts to submit to, and so regretfully passed.
My dilemma? She also said, "If there's anything else I can do to help in your search for representation, please do let me know."
If she knew agents who would have those contacts, wouldn't she have referred me? Do I ask for other agents she thinks might know a good fit or assume she would have told me already? What else could she do to help me? It seemed like an amazing offer which, in the end, I really don't know what to do with other than post on my wall.
- May 21, 2009 at 2:07 PM
- Rick Daley said...
Thanks Nathan, this is an awesome post!
- May 21, 2009 at 2:08 PM
"If the agent passed on your query: Do not re-query with the same project, even if you've revised your query and/or manuscript."
I know more than one person who (after a few months has passed) requeried the same project with a substantially better query, and gotten an agent out of it. (And some very good agents.)
Honestly, I would ignore Nathan's advice. Don't do it more than once to an agent, don't do it if the changes are only cosmetic, but FOR INSTANCE, if your first query was something like, "This is a book about love and death and hope, and it's kind of a crime story!" and the second query is an incisive piece of work focuses on the conflict, requery. Cosmetic changes only--no requerying. Massive revision and restructuring--go ahead.
Just use good judgment.
(And now I see that Nathan says the same thing in the comments trail.)
Just keep this in mind, Nathan: People who don't have good judgment aren't going to be following your rules anyway. :)
I'm sure that makes you feel so much better!
- May 21, 2009 at 2:18 PM
- Deniz Bevan said...
"If the agent passed on your query: Do not re-query with the same project, even if you've revised your query and/or manuscript."
Even if they were part of Be An Agent For a Day and you told them to feel free to submit their query after the contest was over? :-)
- May 21, 2009 at 2:20 PM
- pilot said...
Nat, let's assume the query is good. You've sent two dozens out and got ten requests. So, it must be working. But there are those few agents (the ones you would really like to represent your work) to whom you can't get it…. assistants are fast on form-rejecting.
Well…. now that I think of it, I never queried you…. That's an idea. LOL
- May 21, 2009 at 2:21 PM
- Nathan Bransford said...
The agent may have just been polite, or they may have genuinely wanted to help. But keep in mind when you're querying that the worst they can do is say no. If they come out and say "let me know what I can do to help you" it's okay to come back and say, "Well, actually..." The worst they can do is say no, they're not going to blacklist you or anything.
- May 21, 2009 at 2:21 PM
- Nathan Bransford said...
Then it's okay.
- May 21, 2009 at 2:21 PM
- Nathan Bransford said...
It's just that I don't want to hear again from most queries I pass on, but if you're the type of person who reads the comment thread on my blog, then I trust your judgment. Hence, one policy for the front page, another for the comments section.
- May 21, 2009 at 2:22 PM
- Mira said...
Exposition!! Yea. And very helpful exposition, at that. Thanks, Nathan. This is really useful. I appreciate that you laid out all the scenarious. As always, it's very clear and reasonable and to the point.
I also like that I am now a special person with a special policy.
But I really want to warn you, Nathan, against trusting my judgement. Don't do that.
No matter how many times I read the comments.
- May 21, 2009 at 2:27 PM
- Bane of Anubis said...
Here's a question for others:
Do any of you send queries to non-preferred agents before sending out to your preferred agents?
(I do - to test the waters of my query so I don't completely hamstring myself with preferred agent/s)
- May 21, 2009 at 2:31 PM
- Mira said...
You can trust everyone else's judgement, tho.
I just like to do things differently. I was thinking of sky writing my query across the Golden Gate.
- May 21, 2009 at 2:32 PM
- Laura Martone said...
Bane -
I wish I had thought to do that. I sent letters to my preferred agents first - and then completely revised my query. Oops.
- May 21, 2009 at 2:33 PM
- Laura Martone said...
Nathan -
I know you've touched on this in previous blogs, but how crucial is the personal touch in a query? For instance, while it makes sense for a writer to mention that he/she is a reader of your blog, what should you do if you have no real connection to an agent? I mean, say, you're both from New Orleans, do you mention that fact if it relates to the setting of the novel - or would it be better to just keep it professional and pretend you have no connection?
- May 21, 2009 at 2:38 PM
- Malanie said...
Thank you for the clarification.
- May 21, 2009 at 2:49 PM
- Malanie said...
LOL Mira, I love it. Reminds me of the college grad that couldn't find a job and stood at a street corner (downtown Oklahoma City) and handed out his resume to all the business men. He made the news and landed a job!
- May 21, 2009 at 2:54 PM
- Bane of Anubis said...
Melanie, someone did that here in Portland, too - a recent MBA grad - I'm not sure what happened to her - although I wouldn't have hired her (b/c her signs were handwritten and not altogether professional looking - so Mira, if you do skywrite your query, no hearts over i's or smiley faces in the o's)
- May 21, 2009 at 2:58 PM
- Renee Collins said...
Very helpful post, Nathan. I have a quick question, if that's alright.
A very reputable agent has had my partial for eight months now. As per the guidelines on her agency's website, I send a polite status check after 3 months. Then another two months later. Okay, so I get it, she's not interested in the book. I've moved on. :)
My question is, I am nearly ready to query a new novel and I would still love to work with that agent. Do I query her anyway, even though she's never technically rejected my other book? If I do query her, what should I say?
- May 21, 2009 at 3:00 PM
- Nathan Bransford said...
Totally up to you.
- May 21, 2009 at 3:03 PM
- Bane of Anubis said...
Renee, just out of curiosity, why would you want to work with this agent if she's left you high and dry on a previous submission?
- May 21, 2009 at 3:03 PM
- csmith said...
Thanks for this Nathan, in the hazy far future when I have something actually READY for submission, I will bear this sterling advice in mind.
- May 21, 2009 at 3:20 PM
- Kristi said...
I would think that if agents saw a re-query on the same ms (aside from the exceptions mentioned in the comments), they might doubt that you had anything else written.
Related to other comments as far as whether to query top-tier agents first or last - when I start the process, I plan on starting at the top and working my way down (after having my query critiqued by published authors in my critique group of course). Why wouldn't you try for the best?
I'm still several months out from that whole process, so Nathan, this gives you plenty of time to expand your business to the wonderful world of PB's. :)
- May 21, 2009 at 3:29 PM
- Charlie said...
Excellent advice Nathan. I'm planning on treating the querying process as having one shot to make it. The manuscript will be as polished as it could be and the query flawless.
Shall I skip the glitter confetti?
- May 21, 2009 at 3:30 PM
- Suzanne said...
Nathan, what if your assistant passed on the original rejected query?
- May 21, 2009 at 3:37 PM
- Nathan Bransford said...
I don't have an assistant.
- May 21, 2009 at 3:39 PM
- Bane of Anubis said...
Nathan, I think Mira's gonna volunteer for the job if she hasn't already :)
- May 21, 2009 at 3:43 PM
Did I notice a few differences in opinion between Jessica's post and yours? Maybe I missed something, but I thought the gist of Jessica's post was to explain to the agent that you are, indeed, re-pitching so they don't get confused. She even gave an example of how to do this, after you've revised your query/ms. And your post says don't re-query at all because the agent will always remember you and won't change his/her mind. And if you do, even with another project, don't bother to mention that you've already queried once.
Again, maybe I missed someting.
- May 21, 2009 at 3:50 PM
- Laura Martone said...
I know this is slightly off-topic - it would have been better suited for Monday's "open thread" - but I'm relatively new to the query process and learning as I go how to polish my query for maximum effect. After sending it out to five of my preferred agents, I revamped it (using Nathan's and others' general advice on this helpful blog) - but I'm still puzzling over the matter of personalization. How necessary is it for the query to be personalized for a specific agent? Any tips would be most appreciated. Thanks to all you awesome (and at times, hilarious) bloggers!
- May 21, 2009 at 3:50 PM
- Mira said...
Do you know about the author who held a big funeral for her book? It was turned down 16 times, and she organized a big ceremony for it. It was in the news. I didn't follow the story, so I never found out if all the publicity helped her sell the book.
Here's the URL to the article:
Bane - Don't be silly. I'm not putting little hearts over the i's in my sky writing. Way too expensive after I shell out for the Marching Band and water show.
- May 21, 2009 at 3:51 PM
- Mira said...
I'll be your assistant.
You don't have to pay me.
Heck, I'll pay you.
- May 21, 2009 at 3:53 PM
- Nathan Bransford said...
I don't know Jessica's exact feeling about all of these scenarios, but my understanding of the question she was answering was that the person was asking about re-querying agents who had either seen partials or who had been separately encouraging, in which case it's okay to re-query, and I agree that in those instances it's best to give the agent a heads-up that they've seen it before.
So I'm not sure that we disagree.
- May 21, 2009 at 3:54 PM
- Malanie said...
ROFL, Bane! Hearts and smiley faces, that is hilarious!
- May 21, 2009 at 3:54 PM
- Malanie said...
Mira, no I did not hear of that! Wow!
Nathan, give Mira a chance! Have you seen her blog? She would make quite an assistant, and she is willing to pay you!
Mira, if Nathan says no you can pay me to be my nanny! ;-)
- May 21, 2009 at 4:06 PM
- Hilabeans said...
Oh, I want to volunteer too!
I'd make an awesome assistant, but if Mira's going to pay you, I totally understand if you pick her.
Thanks for the excellent post!
HHS - www.hilaryheskett.com
PS - Did you watch the Bachelorette?
- May 21, 2009 at 4:16 PM
- Marilyn Peake said...
Thanks for posting a summary of your query policies. I realize I've learned a lot from your blog this past year. How to write queries used to be a complete mystery to me. Today, as I read through your list, I found myself saying, "Yup, knew that." Thanks for your very informative blog!
- May 21, 2009 at 4:23 PM
I remember a post by Miss Snark (RIP Miss Snark!) where she basically said break the rules what do you have to lose. I have to say I took this to heart and re-queried agents who rejected my initial query--with a query that had a different tone but it was the same book, same title (I realized that I was missing the mark with my query) ...Anyway, I got several requests that way. (keep in mind I did not include pages)
I also snail queried agents who never responded by email and got most of my outstandings answered.
- May 21, 2009 at 4:24 PM
- Nathan Bransford said...
I have to say, while I'm sympathetic to not getting a query right the first time, all this talk of re-querying with the same project is making me shudder a little, and I think it's at least a part of the reason agents are increasingly moving to "respond if interested" policies.
It's a real struggle to keep up with the queries I'm getting already. I really don't want people to plan on getting a second chance with their query or to use us as a sounding board to bounce around query ideas and styles.
- May 21, 2009 at 4:32 PM
Thanks so much for this great info. And here I was about to type up your comments from SCBWI-WWA's talk about "sparkling query letters." Maybe I'll just point everyone here instead!
So, here's my question: if you read someone's first five pages at a conference like last weekend's, does that count as a query? And so then do all the re-querying rules apply?
Val (who promises not to requery until significant work has been done with her "voice"!)
- May 21, 2009 at 4:41 PM
- Renee Collins said...
Nathan-Thanks. Though the indecisive part of me was really hoping that you would tell me exactly what to do. :)
Bane-I've asked myself the same question. Ultimately, I'm not scratching this agent off the list because all of my research indicates that she is great to work with and a nice person.
I don't know what happened with my partial--maybe it got lost in the shuffle, maybe she just didn't like it. Whatever the reason, I'm not going to let wounded pride stop me from a possible chance to work with a fantastic agent.
- May 21, 2009 at 4:43 PM
- Etiquette Bitch said...
nathan-this does help, thanks.
what if your "source" is essentially the same, but the book is totally different format. ie, i wrote and pitched a memoir, it got rejected, but now, it's not a memoir -- it's a how-to book with only tiny nuggets from the memoir. is that worth mentioning, or just pitch it as a new work?
- May 21, 2009 at 5:02 PM
- Beth said...
Out of curiosity, I queried an agent for my first book and received a form rejection. About six months later, I queried her about my second book. In the rejection, she thanked me for querying again, briefly mentioning my first book. Do agents keep track of query letters? I was really surprised she remembered.
- May 21, 2009 at 5:16 PM
- lotusgirl said...
This was a very helpful post. It's nice to know the time frames and etiquette. I would have thought if the project was completely redone it would be okay to requery the same agent.
- May 21, 2009 at 5:28 PM
- PurpleClover said...
Thanks for the information. I have requeried agents in the past but always with separate projects and usually a good six months to a year passed between projects. Glad to see that is acceptable.
Does your agency bounce queries off each other to make a decision in team meetings or is each person wholly responsible to decide who they bring on?
- May 21, 2009 at 5:40 PM
- Anita Saxena said...
Thank you for spelling out "re-querying" etiquette so well. I know it can vary agent to agent, but these seem to be some good logical rules that I can put up there with "don't put your elbows on the table in formal company."
Thank you : )
- May 21, 2009 at 6:11 PM
- Nett Robbens said...
Hi Nathan,
Thanks for the advice! Quick question: Is it okay (or wise) to re query an agent who was disappointed with a partial, which underwent major revisions months after the original query, and is now a contest finalist? (They weren't thrilled with the characters or writing.) Or is it safe to just move on and query another project?
- May 21, 2009 at 6:13 PM
- Nathan Bransford said...
I'd use your gut instinct on that one. In general it's probably best to stick to the ones who were very encouraging even if they passed, but it's up to you.
- May 21, 2009 at 6:14 PM
- Nathan Bransford said...
In general it's up to the individual agents.
- May 21, 2009 at 6:16 PM
- Nett Robbens said...
Thanks again Nathan, I appreciate your counsel!
- May 21, 2009 at 6:20 PM
- PurpleClover said...
I was only asking because I know of at least one agency that says it is unacceptable to requery with another agent in the same agency because they discuss prospects at team meetings each week. But in that case it would be obvious who was simultaneously querying intra-agency (that's probably not even a real word).
I think it was a smaller agency anyhow. Plus, like you said earlier, each agent has a different preference. I was just curious how your agency did it.
- May 21, 2009 at 6:21 PM
- carly said...
Bane asked earlier if anyone begins their query process with non-preferred agents to test the waters.
I've made a list of the agents I plan to query and then rated each on a scale of 1-10, based on preference and fit. I'm starting to query my "7"s and "8"s. Then, after considering their feedback, will revise and move on to the "9"s and "10"s. Only time will tell, but it seems like a good system in theory!
- May 21, 2009 at 6:29 PM
Good stuff. All good stuff.
And wow! 503 comments from yesterday. Is that a record?
AND...you must be a wicked fast reader in order to get through all of this, especially without an assistant. I'm so impressed. But I beg of you, don't take Mira up on her offer. I'd like to blacklist her comments from your page. A little bit goes a looooong way. :)
- May 21, 2009 at 6:35 PM
- Jarucia said...
This is nearly verbatim what you told a questioner at the recent SCBWI conference during your Sunday session.
Though it isn't 'technically' part of re-querying, but can fall under that umbrella, you and others mentioned the potential pitfalls of 'querying' an editor before querying an agent.
If the editor says 'no', then (as appears to be the case with most houses) the whole publishing house says 'NO' period.
So, if the author moves on to querying an agent, they should disclose any editors they've queried and heard rejections from so the agent knows of any bridges already burned.
I thought this a VERY interesting bit of information.
- May 21, 2009 at 6:49 PM
- allegory19 said...
Thanks for all the info - and not just Nathan - there are some really great questions courtesy of fellow readers. Thanks guys!
Hahaha Minnie@3:35 - sorry Mira, but I thought the same thing today. Maybe not blacklist, but still...
- May 21, 2009 at 6:49 PM
- Nathan Bransford said...
Very good point -- I'll try and blog about that soon.
- May 21, 2009 at 6:50 PM
- Laurel said...
Back off, Minnie. Mira has a fan club.
Nathan seems plenty assertive. If she gets out of hand, he takes her to the woodshed.
Ian, PC, Jo, can I get a little help here?
- May 21, 2009 at 6:53 PM
- Nathan Bransford said...
Let's not turn this into a battle. If anyone has any concerns about someone's comments on the blog (or mine for that matter), as always please feel free to e-mail me directly. But please let's deal with it that way rather than getting into a spat.
- May 21, 2009 at 6:55 PM
- Mira said...
Hi Minnie,
Yes, I've been thinking the same thing the last couple of days. I think there's too much 'me' on this blog. Definitely.
I'm trying to cut back . It's hard - it's abit addictive, and when people address me, I don't want to ignore them.
But I think you're right.
On the other hand, please be nice to me about it. I'm just another writer like you, although one who can get carried away.
- May 21, 2009 at 6:55 PM
- Mira said...
P.C. - thanks for the support, that's sweet and means alot.
But I think everyone needs to be comfortable here. This is Nathan's blog, not mine.
Minnie's right. I'm taking up too much elbow room. I really have been thinking about this all week. So, I'm glad she said something. Allegory, too.
People come here to chat with and get to know Nathan. I'm too active.
And see, here's another post of mine. :-)
It's not a big deal, really. I'll just post less. It's not like I'm leaving.
- May 21, 2009 at 7:04 PM
- Bane of Anubis said...
Carly, sounds like my kind of process... And, for those who think that there's such a thing as a perfect query letter, I say "bollocks!" (though there may be a perfect letter for certain agents (e.g., "I'm Cormac McCarthy - represent me." End of Letter)
- May 21, 2009 at 7:09 PM
- Mira said...
Oh, god, Laurel. I mean Laurel, thanks for the support. I mean it. Thanks.
- May 21, 2009 at 7:09 PM
- Elaine 'still writing' Smith said...
I thought Nathan managed yesterday's anti-thread perfectly. Everything that stayed was in keeping with the 'education and information sharing' aims of the blog.
Useful information as usual, Nathan.
- May 21, 2009 at 7:15 PM
- Laurel said...
I like Nathan's suggestion that we pretend we're grown ups and email each other directly. Most of us post through blogger and you can get through to each other that way-just click the name of the poster. No reason to make anyone embarrassed or uncomfortable in a public forum.
Thanks, Nathan. Sorry for going off topic.
- May 21, 2009 at 7:17 PM
Nathan's blog has several regulars that are part of his blog's appeal. Without his regulars... it wouldn't be the same.
I find Mira and many others to be funny and entertaining.
If someone doesn't want to read what someone else is saying, they can skip them.
- May 21, 2009 at 7:33 PM
- Mira said...
Well, I'm going to post less, but not right now. :-)
Laurel, you're great. Thanks.
In the last week, I have received some of the best compliments about my writing I've ever had in my life. Compliments that were better than I ever expected to receive.
I'm good.
But for today - it's probably BETTER that it came out publically. I think Minnie is speaking what some other people may have been thinking too, and it's better if it's out in the open.
That way I can agree, and we can move on.
- May 21, 2009 at 7:36 PM
- Pattie Garner said...
Wow Nathan, I feel like this post was just for me. Thanks!! :) But you are still my favorite!!
- May 21, 2009 at 7:50 PM
- Elaine 'still writing' Smith said...
Mira - 'back away' from the backing away ... with out 'the humour' and 'the clever' the rest is a lot of 'thank you's'.
However, if things got dull I guess I'd get more work done.
Thanks Nathan for the informative post.
- May 21, 2009 at 7:58 PM
I followed the link you provided to a previous post regarding exclusives in the case of an author agreeing to do revisions suggested by an agent. You believe it should be exclusive because the agent is spending considerable time on the project. Ahem, so is the author - with no guarantee of representation. It gets very complicated when you've already done simultaneous submissions.
So if another agent has the full, it's not fair to let that agent waste time reading it when you're embarking on revisions. And then to make the scenario more complicated, another agent with the partial has just requested the full & makes recommendations that also make sense to you. So what is an author to do? Turn down the other 2 agents even though there is no guarantee of representation?
This very scenario happened to me. I could find no precedent for how to handle it, but I'm a business woman and used my experience w/hiring contractors to guide me. Agent 1 and I had an understanding there were no promises on either side. So I informed the other agents that I was doing revisions. All knew the MS was a simultaneous submission. As far as I'm concerned, I acted professionally and kept my options open at the same time.
At any rate, the scenario I described might make an interesting blog post. ;-)
- May 21, 2009 at 8:01 PM
- Nathan Bransford said...
There's no way to cover every eventuality in a blog post. The most important thing when dealing with a situation like that is just to be very up front with everyone where everything stands.
- May 21, 2009 at 8:04 PM
- Mira said...
Who said anything about giving up the humor and the clever? I just said less.
Of course, I said I'd try to post less. I didn't say it would actually happen.
It's not happening right now.
I'm not good with the self-discipline stuff.
- May 21, 2009 at 8:07 PM
- Alps said...
Thanks for laying all this out so clearly. Good post.
- May 21, 2009 at 8:25 PM
- PurpleClover said...
Not looking to spatt - but since my name was brought up I feel I owe it to Mira to at least say she doesn't bother me. ;)
Of course I'm just as guilty of rambling (especially as the weekend nears and I become a blabbering fool where everyone is my best friend). Nathan indulges us occasionally going off topic so I must thank him for being patient with us! It is important, I think, to keep it professional. Nathan does a pretty good job at making sure it stays that way and if he ever has to correct someone it seems to be for the last time (for them at least).
Sometimes we just get giddy and talkative. I feel I must apologize as well as I've done it myself (including now - we are off-topic ...sorry Nathan)!
Anyways, Mira - I have no idea how old you are but you are like a little sister to me. Cute, funny, and sometimes annoying. But in an endearing way. ;)
- May 21, 2009 at 8:35 PM
- Jen C said...
As someone mentioned above, it's the regulars here that make this blog kinda special.
Mira, I love your comments, and I feel like it's the easiest thing in the world to just skip past someone's comments if you don't really care for them.
Would I rather read a bunch of thank you's, as someone so eloquently put it (can't be bothered scrolling down to give credit, sorry!) or would I rather read about the Ostrich Pickle Conspiracy? Hmmmm.... Tough call... very tough call...
- May 21, 2009 at 8:39 PM
- allegory19 said...
Cheers to Mira - excellent attitude! Thanks!
- May 21, 2009 at 8:48 PM
Please keep posting. Half the time, I read the comments just to see what you said.
Otherwise, I'd just scan them quickly to read Nathan's responses.
- May 21, 2009 at 8:53 PM
- index.php said...
Hello Nathan,
I have a question about a hypothetical case that I'd like to be in someday:
Say I have my eye on an agent but they rejected my query. Later, I submit directly to a publisher and they're interested. Now that my project is a stronger commodity Should/could I re-query the agent with the new information?
- May 21, 2009 at 9:20 PM
- Mira said...
- This comment has been removed by the author.
- May 21, 2009 at 9:28 PM
Oh geez, Mira, SORRY! I didn't mean to bash the comments personally. Only the frequency. I'm very new here. Sorry to Nathan, too. This is just the kindof stuff I hate about blog comments, personal conversations. So I'll shut up and just go back to reading. Just my luck to leave a comment for the first time and it's controversial! Figures.
Have a good weekend everybody. Signing out.
- May 21, 2009 at 9:43 PM
- Mira said...
Minnie, it's cool. I think you forgot I'd be reading what you said. But you started a good conversation.
Don't go.
But anyway, now that I'm done talking about how IMPORTANT I am - whether I post or don't post, let's get back on topic.
Nathan, I have a very important question for you.
How effective is this in a query letter? I'm asking because I'm going to send you a query letter next week, and I'm trying to expand my options.
Would blackmail give me that edge?
Just wondering.
If it would, I also need you to tell me something so I can blackmail you.
Of course, I could just threaten to post more. That might work.
Ha. ha. Couldn't resist.
- May 21, 2009 at 10:09 PM
Good post. Thank you.
- May 21, 2009 at 10:30 PM
So, what if you were represented by an agent at a big-name agency that shall remain nameless. ; ) You and the agent parted ways a couple of years ago. Would it be considered strange/inappropriate to query another agent at that same agency (with a different project, of course)? And should I mention my former agent by name in the query?
Many thanks for your time!
- May 21, 2009 at 11:33 PM
- Mira said...
Boy, that post above really made it sound like I think I'm all that.
Well, I do, but I don't need to advertise it.
Nathan, it's your blog, it's your call.
Boy, for someone who wasn't going to talk, I sure talked alot tonight.
Think it's time for bed.
- May 22, 2009 at 12:03 AM
- Lyn Miller-Lachmann said...
Thanks for the information, Nathan. I always thought that if an agent rejected your partial or full manuscript, you'd burned your bridges with that agent and couldn't re-query on that or any other project.
- May 22, 2009 at 12:13 AM
- Nathan Bransford said...
Yes, it's okay to re-query the agency, but I would definitely mention that you were previously represented there (and who it was) to avoid awkwardness all around.
- May 22, 2009 at 1:21 AM
- Patrick Rodgers said...
I can understand why some might re-query if not entirely condone it. If there is an agent that you are just dieing to work with for any number of reasons and then to have them send a form rejection it may entice you try to get them as your agent later on.
Take for example Nathan for many readers of this blog and aspiring writers he may be someone you just really want as an agent. I think that you can almost feel a connection to Nathan because of the blog. He's not some faceless agent you got out of a book like Writer's Market or from Jeff Herman. There is a connection here and that is hard to resist.
The first agent I will be querying is Nathan for many reasons (I think I am still a month or two away from querying though, editing is kicking my ass). The first being the connection his blog gives me as a writer. Secondly because he takes email queries and answers them so very promptly. If you are going to get a NO might as well get it in 24 hours instead of having to wait 2-3 months.
So if I get a NO, maybe I can hope my winning personality will win him over on this blog and he will be like that is one funny bloke why did I ever tell him no when I re-guery ;)
- May 22, 2009 at 3:09 AM
- PurpleClover said...
Patrick -
I think it is only fair to query blogging agents first since they are the ones that have put us in the best position for querying. All their advice has helped me improve by leaps and bounds so I think they should get first dibs. ;)
Good on ya mate! (Sorry I just had to after reading your "bloke" reference! I'm not even sure that was the right context...lol)
- May 22, 2009 at 7:18 AM
What if you send a partial (when publisher requested a complete ms)with questions before finalizing your story and after six to seven months, still no rejection - even after sending two reminders to request status of your project? When is the right time to make the call?
- May 22, 2009 at 8:25 AM
- Jo said...
All very useful stuff to know especially re-querying an agent with a different project as that is what I will be doing in a few months.
- May 22, 2009 at 10:26 AM
I was the anon @ 12:50 PM
Just wanted to say thanks for answering.
- May 22, 2009 at 10:55 AM
Hey this blog is fantastic. I'm currently about to start the process of querying and have a problem/question. Printing copies of manuscripts is pretty pricey and printed mine, then realised there's something I need to add to one part. I've read that it's okay to just print the changed pages, and if the page numbers don't line up anymore, have, for example, page 124a, 124b etc. What's that like from the perspective of a prospective agent? Would it give the impression of a cheapskate/amateur or is that fine? Thanks.
- May 25, 2009 at 5:44 AM


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Arcadia, CA, March 13 , 2014 -- HRTV (R), The Network for Horse Sports, will continue its partnership with HITS, Inc., when it presents the live webcasts, via www.tvg2.com, of the first two jewels of the HITS Triple Crown of Show Jumping, beginning with the AIG $1 Million Grand Prix, presented by Lamborghini Newport Beach, from the HITS Desert Horse Park in Thermal, CA this Sunday, March 16.
The live webcast of the AIG $1 Million Grand Prix is set to begin at 2 p.m. ET.
Slightly more than a week later on "Million Dollar Monday" -- March 24 -- HRTV will offer the live webcast of the Great American $1 Million Grand Prix from HITS Post Time Farm in Ocala, FL, with online carriage scheduled to begin at 6 p.m. ET.
Both of these top-flight competitions are key qualifying showdowns for the sport's culminating event -- the $1 Million Grand Prix, which is held annually in early September at Saugerties, NY.
The AIG Grand Prix from Thermal attracts 40 of the best North American and International equestrians each year, and often features former World Cup champions, Olympians, and established veteran stars. Among those expected to compete this Sunday include: Richard Spooner, Mandy Porter, Enrique Gonzalez, Nayel Nassar, Ashlee Bond Clarke, Rich Fellers, and many other stars.
Meredith Michaels-Beerbaum won this event in 2013.
About HRTV
HRTV, The Network for Horse Sports, is a television-based multimedia network dedicated to equine sports. HRTV's programming features horseracing from top U.S. and international racetracks, as well as a wide range of world-class English and Western horse competitions, news, original programming and award-winning documentaries covering the world of horse sports. The live stream of HRTV is available on a subscription basis to high-speed Internet users worldwide at www.tvg2.com. In the U.S., the HRTV television network is available via telco, cable and satellite in over 19 million homes.
Contact: Scott Cooper, HRTV, 609-391-8166
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text:
^That sucks. The tiered plan data allotments are a joke, unless you're on wifi at home, work, and most places you go to.
I just got 1.5 down and .4 up with my i4 on 5.0.1, 4/5 signal bars, speedtest.net app, just over 3gb of unlim.
I used to average about 20gb during the mywi days until at&t cracked down on tethering. IMO if you pay for data then you should be able to use it as you please.
I think I'm being throttled I did 2 tests on 3G and 1 test on wifi. It makes sense. Now I'm guessing that's why on my lunch break it sucks to load anything.
1 test: 0.15 download speed on 3G
2 test: 8.51 download speed on wifi
Ping 128 ms
DL 0.46 Mbps
UL 0.87 Mbps
4S, Wifi
Ping 18 ms
DL 13.82 Mbps
UL 4.95 Mbps
I think they're ******* me. I have a grandfather contract which I'll admit is wasted money because between 1/9/12 and 1/17/12 I've only used very minimal cellular network data as I am typically near wifi, 7.7MB Sent & 18.2MB Received. They are ******* me, right?
My data says 895.12MB used
Try rebooting yet?
My twitter, this app, emails and stuff aren't working unless I reboot then the data comes in brand new iphone4s, freakin AT&T I'm on unlimited it doesn't matter if I'm on wifi or not bazar
And dial *3282# to get an accurate idea of how much data you've used in your bill cycle.
I'm also grandfathered since I've been with AT&T since the 2G turtle. I get that top %5 almost monthly and this is from just surfing the web and watching YouTube during lunch at work. I hope it isn't true that they throttle by the area you live in....come on I'm in Los Angeles.
The data cap PER AT&T CONVERSATION is "any use greater than 2gig per month" is considered the "top 5%."
Now, according to an email some are getting, it's considered to be 12 times the limited (2 gig per month) users. Which,I'm not a math genious here, equals 24 gig.
So, AT&T, which is it?
Here's the part of the email I mentioned:
As mentioned on a previous bill, we're also taking additional, more immediate steps to help address network congestion and improve reliability. One of these steps involves a change for some customers who use extraordinarily large amounts of data in a single billing period - about 12 times more data than the average smartphone user.


text:
Choosing a car seat for your growing baby
Once your baby has outgrown their baby car seat, you'll need to buy a bigger one. Just as with car seats for newborns, there are some important things to consider when you're buying a new car seat. If you need information on car seats for your newborn please see our car seats guide for your newborn baby.
Know the law about car seats
As the driver you are legally responsible for using an appropriate child car seat for children up to 135cm (4ft 5in). Safety experts go even further and recommend using a car seat for children up to 150cm (4ft 11in).
You need to check that:
- Your car seat conforms to the United Nations standard, ECE Regulation 44/03 or ECE 44/04 (look for this on a label on the seat)
- The car seat is the correct type (known as 'group') for the weight of your child. Weight is more important than age when choosing the right car seat
- Your car seat is correctly fitted in your car. Follow the car seat manufacturer's instructions carefully when securing your seat.
For more details on the law for using car seats, including rare exceptions, please visit the Which? site.
Choose a car seat for your growing baby and toddler
When the time comes to change your newborn baby car seat for a larger one, there are three groups to choose from (depending on which baby seat you originally bought).
All of these are forward facing but remember that keeping your child in a rear-facing car seat until they are 13kg (29lb) is considered safest.
Buying a combined group car seat (such as 1 and 2) can be a money-saving choice, as it will last your child for longer.
|9-18kg (20-40lb)
|9 months-4 years
|1 and 2
|9 months-6 years
|9 months-4 years
|15-25kg (33-55lb)
|4-6 years
Choose a booster seat for your child
Once your child has outgrown their Group 1 and 2 seat, you'll need to use a booster seat until they're at least 36kg or 135cm tall. This is because they are still too small to benefit from the full protection of an adult seat belt, which should sit comfortably across their collar bone, shoulder and pelvis. Booster seats can be just a backless base to sit on, or a high-backed model, which helps protect your child's head in the case of side impact.
Combination Group 1, 2 and 3 car seats use a 3- or 5-point harness until your child is around four years old. Then, they can be converted into a booster and used with an adult seat belt.
|15-25kg (33-55lb)
|4-6 years
|2 and 3
|15-36kg (33-79lb)
|4-12 years
|1, 2 and 3
|9-36kg (20-79lb)
|9 months-12 years
Fit your car seat correctly
Fitting your car seat safely and securely is crucial. You'll find detailed instructions with the car seat and many seat manufacturers have instruction videos on their websites. Here are a few general tips:
- practise getting the car seat in and out of your car before your child uses it.
- check the car seat belt is long enough to secure the child car seat and that it can go through the slits in the car seat without any obstructions.
- check your car has ISOFIX mounting points if you plan to use an ISOFIX base.
- adjust the integral harness every time you travel. It should fit snugly, with no more than a finger's space between the harness and your child's chest.
- checkthe harness straps in a forward-facing car seat are level with, or slightly above, your child's shoulders.
- check that the car seat has minimal forward or sideways movement. When you open the seat belt buckle, the car seat should spring upwards slightly.
- make sure the seat belt isn't twisted.
- allow seat belt buckles to lie against the child seat. Pressure on the buckle during an accident could make it fail. Only the fabric of the seat belt should touch the car seat.
- use a car seat that makes your child pull up their legs to fit in. If they can't stretch their legs comfortably you have fitted the seat incorrectly, positioned the front seat too far back or bought the wrong group car seat.
- buy second-hand car seats. Signs of being in an accident aren't always visible.
- let metal buckles get hot in direct sunlight. Use a window blind to prevent this happening, or cover buckles with a light fabric. Be careful not to let your child overheat.
- use a cushion or other makeshift support under your child instead of a booster seat. It won't do the same job.
- allow too much slack. The car seat shouldn't be able to move too far forward or to the side. If it can, check the instructions, move the car seat to another position or consider returning the seat and buying another one.
- use a lap-only seat belt. Child car seats are designed for use with a three-point diagonal seat belt (or ISOFIX system) and using a lap-only one could cause the seat to flip over in a crash.
- allow the front seat headrest to touch your child's car seat. If it does, remove the headrest. Don't ever use the headrest to 'jam' the car seat in place. The car seat needs to move forward in a crash to protect your child's head and neck.
Check restraint options
Generally, Group 2 and Group 3 booster seats use your car's normal seat belt to secure both the seat and your child.
If you need information on car seats for your newborn baby please see our car seats guide for your newborn baby.


text: Exercise | September 2013 (Revised September 2016) Process Analytics Simulation: Solutions Robert S. Huckman and Roy D. Shapiro A set of exercises and instructions to be used with the Process Simulator software made by Pro Model. These exercises allow students to investigate the impact of variable processing times on the performance of simple in-line processes. Includes color exhibits. Keywords: Operations; Business Processes; Performance; Mathematical Methods; Citation:Huckman, Robert S., and Roy D. Shapiro. "Process Analytics Simulation: Solutions." Harvard Business School Exercise 614-029, September 2013. (Revised September 2016.) View Details CiteView DetailsPurchase Related


text:
These are in no particular order ... they are posted as they come in, with the most recent at the top
Adapters? Primers? what works with what ????
PE (Paired-End) Genomic DNA primers work for GAII and HiSeq and can be read as a single read or paired read. The side 1 paired read site is the same as the single read site. We like these, we stock them at the core lab and sell them to you at our cost. TruSeq primers will work for the assays they indicate on the Illumina site and in the product protocol. For example, TruSeq DNA primers can be used for single or paired reads. However, TruSeq RNA primers can only be used for a single forward read. IF YOU USE THE ILLUMINA ADAPTERS WITH THE INTERNAL BARCODES, AND YOU WANT THOSE READ, YOU MUST ORDER A MULTIPLEX READ when you submit your sample. This is an extra priming and sequencing read and will cost extra. See the section just below this if you are interested in doing the barcoding within the main fragment read (we like that since it's cheaper and quicker and works well).
Barcodes? Indexes? How do I use them? -- SEE BARCODE VS INDEX PAGE
Both Illumina and ABI-SOLiD instruments can read barcodes and indexes. There are 2 ways this works (usually). You can add an index or barcode to your sequence when you build. This is generally added to the 3' end of the upstream adapater so that this is the first 4-6 bases read during the sequencing run, then you sort the data by these first bases into your groups. If you do this, keep them to 6 or less bases and please design them to be as clear as possible. We recommend that you design in such a way that if one base were mis-read or the first base was missed, you could still tell which bar code it is (you can also look at commercially available or previously published lists and use those). Additionally, please design a sequence (and check) that is NOT part of the sequencing primer or part of your PCR primers for obvious reasons. The other way to do barcoding/indexing is to insert the index sequence into the attachment oligo/linker and perform and additional sequencing step to prime and read the sequence in the linker. This will cost more of course since there is an additional sequencing read. It will also take longer to collect data and do the basecalling. If you can spare the first 4-6 bases of sequence read, we recommend the first option. It's faster and cheaper. If you use the linker-based barcodes with the additional sequence read, please use the sequences provided in the support documents from ABI and Illumina (depending on which platform you are building for). Both Illumina and ABI provide the sequences necessary to add these to your library construction.
I heard there is a special discounted smallRNA option for DeepSeq?
Yes, we are running 30 base reads on the GAII for $500 per lane (1 sample per lane). We need 6 (or 7) samples to do this, so if you have a bunch or there are others in the queue to join in, we can do this type of analysis for you. The library must be built using the small RNA library protocol on our webpage (in the services section, Mello Lab protocol). Additionally, the linker sequences used for this are only compatible with the GAII instruments.
Why are you sending me the BioAnalyzer data for my sample? Am I billed for that?
The BioAnalyzer trace on a High-Sensitivity DNA Chip is part of the initial QC done by the core lab before your sample is put on one of the next-gen sequencers. We send it to you for your information and as part of your data set. If there is a problem with your sample which might affect it's performance, we note it in the email and you have the opportunity to adjust the sample (concentrate it, further size-select, etc). A quick response (within 48 hours please) to this is important. Unless your sample was a total bust, we will keep moving forward with the analysis unless we hear from you within 48 hours. You are not billed for BioAnalyzer analysis which is part of sample QC, however if you want to have samples run on the BioAnalyzer for any reason, the MBCL offers a very economical BioAnalyzer service for RNA and DNA samples (http://www.umassmed.edu/MBCL). If a user submits samples "to see if they are good" in order to get BioAnalyzer info before moving forward or to assist in the selection of which samples to have sequenced and which to withdraw, we will invoice for the BioAnalyzer run(s) as these use reagents and resources. If you need to see how your library looks when finished, or better yet, during stages of construction, we strongly recommend using the BioAnalyzer service at the MBCL (see link above).
Why do I see lots of "BBBB" in my fastq quality info when the sequences map great and appear to be good reads?
This is a very good question, one which we at the core lab have been asking as well. It appears to be an "undocumented feature"!! There is some chat about it online (try this for a start, thanks David,http://news.open-bio.org/news/2010/04/illumina-q2-trim-fastq/ ). We see it most often when there are non-random bases at the ends of sequences. Non-random bases (e.g. linkers) raise flags for the analysis software. The base calls maybe (and usually are) good, but they don't look like the rest of the run to the instrument. We welcome feedback about your experiences with this so we can share details with Illumina. Please continue to keep us updated about your data analysis experiences.
Why do HiSeq run types (SR100, SR200, PE50, PE100, PE200) cost more than the runs on the GAIIs?
The reagents cost more, the flow cells cost more and overall they cost more to operate. On the other hand if you build a good library, you'll get a lot more data! As with the other instruments the number of good reads is determined by the LENGTH of the library (length is figured into how many clusters can be seeded), how tightly the library is sized (see below), and the content and quality of the library. On average, during the validation runs we are seeing 4-10X more reads per sample, some even higher.
What are the prices for the various types of runs/analyses available?
Pricing for UMass investigators can be obtained by sending an email to
email@example.com and asking for a price list. Investigators at other institutions should contact us for pricing as there are different schedules set by the administration for outside users. WHY AREN'T THE PRICES ON THE WEBSITE? These web pages are viewable outside UMassMed and our agreements with vendors for discounted reagents etc (which we pass on to our users) prevent us from publishing those prices outside our group.
How long is the queue? (and all variations on this question)....
The queue (or wait) has several components. If you are asking "How long from the time I give you my sample until I get my data?" Then we must include the time to perform QC on the new sample and any additional clean up required, the time spent building clusters/seeding flow cells/preparing emulsions and doing the WFA (work flow analysis for quality control), the duration of the imaging process on the instrument (varies with the length of the reads, multiplex read and any paired end read), the time to transfer the raw information to the High Performance Computing Cluster and the computational time required to resolve image information into basecalls and preliminary alignment to reference genome. The "queue" for a short-insert, well made library is usually a couple of weeks. The queue for a library with long inserts which is poorly made or which has a wide range of insert sizes can be over a month, especially if there needs to be effort spent on getting it into a useable condition. We send you the results of the BioAnalyzer evaluation for your records and if there are concerns at this point we contact you immediately. The sooner you can respond and help with any adjustments, the quicker the sample can move to the next step. Our new LIMS (part of the summer expansion of the DSCL) will have a status bar which indicates where your sample is in this process.
How can I get my sample through the queue faster?
Build a good, tightly-sized, clean library and share information with us about any residual linkers or primer sequences, barcodes, adapters, polyA stretches, even the results of your test sequencing of the Topo clones can be helpful.
What about the number of samples in the queue before mine?
We operate on a first-in first-on basis. With our new instrument additions and the retasking of certain instruments for specific run types, we are not experiencing significant delays due to backlogs of samples. If a user needs to analyze an inordinate number of samples which would set back everyone else, we will work with them to create a schedule that does not negatively impact the other users. (So if you have 120 samples, email us well in advance please).
What's up with the new data pick up system and WHERE the %#@& is Isilon?
The isilon "farline" storage is attached to HPCC03 (one of the head nodes of the High Performance Computing Cluster). HPCC03 is dedicated to moving data and is the place where all your rsync, rcp, scp, copy etc should be done. You can get there from the other locations on HPCC by
$ ssh hpcc03
your passwords etc are still the same as in other locations on the cluster. Once you are on hpcc03 you can cd into /isilon/deepseq/{LABNAME} and copy your data to your own space in /nearline or the R drive or directly to /scratch. Remember to ssh back to hpcc01 before you need to run anything or submit jobs to the cluster.
What's up with the SOLiD? I heard ABI is replacing them with 5500's and that there is some cool new RNA analysis stuff they can do?
Our SOLiD has been upgraded to a SOLiD4, the last upgrade before being totally converted to something else! The newly renovated instrument has been renamed "The HMS Beagle" in honor of Charles Darwin's science discovery vessel (SOLiD info can be reached from the nemo home page on the SOLiD link). A user workshop on May 4th will cover the changes and present the new tools available. Briefly, the Gene Expression tools and Transcriptome Analysis tools are being set up and we are working with investigators who have projects that would benefit from initial deep sequencing of a few samples to determine which Taqman Assays or microarray tools would be best suited for a high throughput analysis of many samples. The new SOLiD tools include new internal controls for more accurate copy number calculations as well as rRNA depletions and library building kits (including paired end!). Other analysis types are still supported.
How can I get some assistance or consultation on using SOLiD for Gene Expression or Transcriptome analysis?
Our Applications Specialist is on campus the 3rd Friday of each month and is available to meet with you and/or your lab. Please take advantage of this special service. If you'd like to meet with him, please email Ellie and get on the schedule (firstname.lastname@example.com)
When will my sample be run? How long will it take? Why don't I have my data yet? Can I move up in the queue? etc etc
We operate on a first in, first on basis for our investigators. However we cannot always control the SPEED at which the queue moves forward. If instruments are not performing up to specs, they are taken off-line and serviced (otherwise you get bad data). When there are issues related to infrastructure, computing issues, instrument failure, power failure, etc. and we need to reset a run, this also delays the queue. The lab staff cannot change the queue, so please don't ask them.
What can I do about getting my data as fast as possible?
Make the best library you can! The guidelines for library construction and validation are there to ensure good performance. The Topo cloning and pre-sequencing is important to determine whether the linkers, adapters, sequence priming site, and attachment sequences are in place. The sizing guidelines are extremely important (see FAQ below about sizing). If your sample covers a wide size range, it's better to make several size cuts and turn several sub libraries. When libraries don't sequence well, they end up getting reworked and rerun, which translates to more expense for the investigator and more samples in the queue.
In the ChIP protocol from the core lab, what do you mean by "reversing the orientation of the column" when you elute?
This refers to rotating the plate or column 180 degrees so that the area near the spindle is now at the furthest point from the center of the rotor. This does not mean to turn it upside down!
Is it important to gel purify the sample after ligation of the Illumina adapters and before amplification with the PCR primers?
Yes. It is important to run the ligation products on a gel and collect the insert plus adapters, EVEN IF YOU HAVE TO GUESS where it is based on the size markers. If this step is skipped, there are many more primer-dimers in the library! ALso, run the markers and other samples with at least one empty well in between.
Is it necessary to order the adapters and primers for the Illumina libraries from the vendor or the core lab or can I make my own?
Adapters can be home-made and in fact this is the best way to add an internal barcode or index, just be sure the phosphate is on the 5' end of the downstream adapter. The PCR Primers bring the attachment sites to the library. They also contain modifications (e.g. LNAs) to strength attachment during bridge amplification, and to provide the appropriate cleavage during the reverse bridging step (paired end analysis). The PCR primers should be obtained from Illumina. The core lab does buy them in bulk and will sell them to you at our cost.
Which version of the pipeline was used on my data? How do I tell? Is there documentation for the different versions of the pipeline?
The numbers after Bustard or Firecrest indicate which version was used. The documentation for these is available through the BioTools website. There is also information on the UMass Wiki. Links follow.
If my run fails, do I have to pay for it? Why am I billed for a run if it wasn't as much data as I need?
You pay for a "run" not a fixed amount of data. Billing is not determined by the output, in other words you don't pay by the megabase. Billing is also not dependent upon performance. We are an at-cost not-for-profit facility. Running your sample uses reagents and supplies so you are billed for the sample-run. If there is a failure due to reagents or instrument performance we will re-run THAT sample again at no additional charge. If the failure was due to library construction issues, e.g. you used non-modified home-made primers, or there was some other problem related to the sample, you pay for any re-runs (after the problem is fixed, hopefully).
What is the difference in the Single-Read and Paired-End adapters and which is best?
Single-Read Primers and Paired-End Primers are the same price. The Paired-End Primers can be used for Single-Read analysis, but you must tell us when you submit your sample that you only want the forward read!!!! The Single-Read Primers can only be used for single reads. If you are not sure, get the PE Primers, they go both ways! If you're building small RNA libraries, check the services page for the protocol and use the primers described there.
Is there any way I can evaluate the run quality? Do you have anything I can run on my own computer to look at the data without using the High Performance Cluster (HPCC)?
We are starting a collection of things, and looking for suggestions, click Resources in the left nav. There is also information at BioTools and on the Wiki.
What types of Illumina and SOLiD analyses are available right now?
At this time we have 2 Illumina GAII's, 1 ABI SOLiD 4, and 2 Illumina HiSeq 2000's (single read 100 and 200 bases and paired-end reads 100 bases). There is another instruments coming into service later in 2011 (stay tuned). The SOLiD 4 is available for either an entire run or a mixed barcoded run. All ABI kits are supported.
Do I really need to size my library and keep it +/-25bp from the median size?
YES, it's a very good thing to do if you want the most and best sequence possible.
Cluster formation is more even if all fragments are the same size, we load based on the largest fragment in the group so if the size spread is wide you'll get fewer clusters/sequences, and cluster detection is more accurate when all clusters are the same size (wide range of fragment sizes results in wide range of cluster sizes). Additionally, very large fragments don't stay denatured as long and will often just roll through or even interfere with the annealing of the smaller fragments.
Why are you so picky about all these gel purifications and being so "clean" during library prep?
Because a little bit of garbage going into the library means MILLIONS of junk sequences coming out of your analysis!


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Federated Insurance, NAEDA's endorsed provider for business liability insurance and risk management services will conduct a special Designated Risk Management Seminar for equipment dealers. The seminar will be held July 15-17, 2013 at Federated headquarters in Owatonna, MN.
Equipment dealers are interested in controlling losses and those companies that are most successful at controlling their losses have designated a key person as their risk manager. The challenge is that it's typically not a full-time position. The responsibility often falls on someone who has other full-time duties to fulfill. This is where Federated's Designated Risk Manager Seminar can be so valuable. The Designated Risk Management seminar targets specific risk management concerns for our industry and Federated understands the risks inherent with equipment dealers.
There is no charge for association members to attend this seminar as this training is another association member benefit thanks to our partnership with Federated Insurance. Attendees, however, are responsible for air and ground transportation to and from Owatonna, Minnesota, and lodging in Owatonna.


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Two years later in 1971, they moved their headquarters to their present location in the traditional Station District on the outskirts of Haro. Bodegas Muga has continued to grow as the years have passed but it has never lost the spirit or aptitude of a family-run company.
The winery controls every step of the viticultural and vinification process from the vineyards to making their own barrels and fermenting and aging the wine entirely in oak. Muga is one of only six estates in the world that owns its own cooperage and they import the oak directly from the United States and France. Bodegas Muga is one of the oldest, most elegant and traditional Rioja producers. View all Bodegas Muga Wines
About RiojaView a map of Rioja wineries (ree-OH-hah) Spain makes some of the best Tempranillo-based wines in the world. Once the only DOCa (recently joined by Priorat in 2001), Rioja is divided into 3 sub-regions: Rioja Baja, Rioja Alta, Rioja Alavesa. There are 4 red varieties and 3 white varieties allowed in the Rioja DOC. Tempranillo definitely takes center stage, followed by Garnacha (Grenache), which is sometimes added for body, then Graciano and Mazuelo (Carignan). The region also makes roses. For whites, the main grape is Viura (or Macebo), producing fresh, early-drinking wines. Malvasia, the grape that was once the most planted white, is found less often.
Notable FactsThe Rioja wine trade is somewhat confusing. Grapes are typically brought to a merchant's bodega from one of the 20,000+ growers in the region, or via a cooperative. The wine is then bottled and labelled by that bodega. Rioja's Consejo Regulador keeps track of all vineyards and bodegas to make sure they are following the DOCa regulations. Put in place to ensure quality, the system also controls prices.
As with the rest of Spain, the wine label may state Crianza, Reserva or Gran Reserva, depending on barrel & bottle maturation. Crianzas are usually found within two years of the vintage and offer fresh, ripe wines. Reserva and Gran Reserva will be found a few years after the vintage, as the bodega will be aging the wines in barrel and bottle before release. Both typically show more secondary characteristics of spice and oak ageing.
About Spain
The most popular red varieties of Spain include Tempranillo and Garnacha (Grenache). Whites don't garner quite as much recognition, but there are some regional varieties not to be missed, like Albarino and Verdejo. The popular red regions of Spain include Rioja, known for its outstanding wines of the Tempranillo grape; Ribera del Duero, producing high quality reds from Tempranillo and Garnacha; Galacia, with the sub-region of Rias Baixas, home to the deliciously crisp and floral Albarino grape; and Priorat, a region increasing in popularity with its high-quality cult reds. Other regions of note are Rueda, growing the Verdejo grape, La Mancha, a wide desert region, covered in the most planted white variety in the world, Airen, and Jumilla, making wines based on Monastrell (Mourvedre).
Spain's wine laws are based on the Denominacion de Origen (DO) classification system, devised in the 1930's. A four tiered system, the most basic level is Vina de Mesa (table wine) followed by Vino de la Tierra (country wine), DO and at the top DOC. Currently, only Rioja and Priorat have DOC status, while over 65 DO's scatter the country.
Most DO regions are classified and regulated by how long they age the wines. On a red wine label, one may find the terms Crianza, Reserva or Gran Reserva, denoting the wine's barrel and bottle time. Crianza is usually two years between barrel and bottle (the time in each depends on the DO and/or the winemaker), Reserva up to 4 years and Gran Reserva 5 – 6 years. Classifications of each region and wine are controlled by the region's Consejo Regulador.


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Determine your face shape with this easy 4-question quiz!
For accurate results, choose 1 option from the following 4 questions using a photo (or your mirrored reflection) where you are facing front on with no hair covering your face.
For more tips about getting the best results, see our accompanying article, How to Determine Your Face Shape.


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Created for performance, built for durability, and styled by fashionable trends, these Ariat Women's Showdown Western Boots are great for all Cowgirls. Created for riding and working, these boots are made with a ATS footbed that has a moisture wicking system gel inserts for shock absorption and stability for long hours on your feet. The wide square toe and walking heel is comfortable and great for riding. The smooth leather heel counter and spur ridge make this boots ideal for wearing spurs. Built with premium full grain leather and duratread outsole, these boots are durable and will withstand rough and tough wear. The screen print designs on the shaft and leather leopard heel counter give these boots a fashionable and funky look that is taking over western fashion.
Style(s): 10009542
Ariat Boots quickly became known as the most innovative and performance riding boots. They provide the same technology in men's riding boots, women's riding boots and kids riding boots. They totally redesigned the traditional outsole, arch support and last shape and took Ariat Boots to the next level in riding boot technology. While building the technology into these western riding boots, Ariat had hundreds of top riders help design and develop their final product.
Today Ariat is the world leader in performance equestrian footwear as well as offering the most stylish western boots on the market. Ariat Boots offers a wide range of products from English and Western riding boots, work boots and casual boots. Their forward thinking and performance lines outfit today's most avid equestrian riders and enthusiasts.
If you are the professional rider who absolutely demands performance boots or the novice who likes to ride around from time to time, Ariat definitely has the boots for every man, woman or child. We have all of the top styles including the Fatbaby boots, Heritage Western, the Dixie and the QuickDraw. These styles are all must have for men's western boots, women's western boots or kids western boots. The casual look and performance driven Ariat Boots will provide you with long comfy rides in the country or a relaxing day in the sun. Get yours today


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Wheeler records first Double-A win
Mets top prospect yields two hits, fans six in fifth start of '12
By Ashley Marshall / Special to MLB.com | April 29, 2012 5:47 PM ET
The Mets' top prospect allowed a run on two hits and three walks while striking out six over 5 2/3 innings Sunday as Binghamton shut down the Erie SeaWolves, 7-1.
"It was great, man," said Wheeler. "It took a little bit, but my offense helped me out today and my defense got behind me.
"Obviously, this was not my best start, but I was excited with my off-speed stuff. My changeup was the best it has ever been."
Wheeler (1-2) lowered his ERA to 1.75, which ranks fifth in the Double-A circuit. Despite pitching well over the first three weeks of the season, he found himself as the only member of the B-Mets' rotation without a win.
MLB.com's No. 28 overall prospect had allowed only four runs over his previous 20 innings -- one run or fewer in each of his last three starts. But Binghamton had scored only four times when Wheeler was on the mound.
Benefiting from a mechanical tweak and improved offensive output, the fortunes of the Georgia native changed Sunday at Jerry Uht Park.
"I've been working hard with pitching coordinator Ron Romanick and he showed me a new grip for my changeup, kinda like a split-finger," Wheeler said.
"The ball was trailing off, so it was hard to throw strikes. But he told me to make one minor adjustment so that my fingers were on top of the ball instead of just on the side. When my fingers were on the side, the ball tailed to my arm side, but I wanted it straighter."
Binghamton plated two runs in each of the first two innings, added one in the fourth and two more in the fifth. The support proved more than enough for Wheeler, who came to the Mets last July in the deal that sent Carlos Beltran to the Giants.
The 21-year-old right-hander faced one batter over the minimum through three innings before working into and out of trouble in the fourth. Brandon Douglas drew a leadoff walk and Rob Brantly singled to right field. Jordan Lennerton's groundout put two men in scoring position for the SeaWolves, but Wheeler fanned the next two batters to end the threat.
"It definitely gives you more confidence because you can pound the zone a little bit more and not worry about being too fine," Wheeler said about pitching with the lead. "You're not always on your toes. You're more comfortable and it allows you to relax.
"Of course, you want to win every game, but even if you go out and do your best you will not always end up with a win."
After a 1-2-3 fifth, he gave up a one-out double to Douglas in the sixth before retiring Brantly. Reliever Jeff Kaplan lost the shutout bid when he yielded Lennerton's double off the left-center field fence.
"I hate giving up runs, I think every pitcher does," Wheeler added. "I had a 95-pitch pitch count and I think I had 12 left going into the inning, and the first guy fouled off a good lot of them. The next guy up after I came out, Lennerton, I had his number and wanted to stay in, but I had to turn it over to the bullpen."
Reese Havens, the Mets' No. 7 prospect, went 3-for-4 with a two-run homer in his season debut and Matt Den Dekker (No. 16) tripled, doubled, drove in two runs and scored twice.
Erie starter Mark Sorensen (0-2) was reached for four runs on six hits and four walks over two innings.
Ashley Marshall is a contributor to MLB.com. This story was not subject to the approval of the National Association of Professional Baseball Leagues or its clubs.View More


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When a person is too young to make decisions or is incapacitated and can no longer care for their own interests, guardian law allows the court to appoint someone to make legal and welfare decisions for them. A legal guardian may be appointed as a limited decision-maker or an all-purpose decision-maker. For example, children whose parents are divorcing often have limited legal guardians help decide custody issues. Nursing home residents may need a general legal guardian to make all their welfare decisions for them. Guardianship lawyers advise you about the appropriate legal guardianship for your particular circumstances. They also help with guardianship forms and other legal paperwork, and represent the guardian once one is appointed.


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Aeroquip FBM4236 Starlite Crimp Fitting, 90 Degree, -16 AN
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Aeroquip's incredible Starlite racing hose and fittings are up to 45% lighter than stainless steel braided hose and reusable fittings. Can be used with fuel, lube and coolants. Working temperature is -45° to +300°F and have a 200 psi working pressure.
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Kendra Wilkinson Talks Hotness
Posted Thursday April 19, 2012 4:43 PM GMT
She was having a blast at US Magazine's Hot Hollywood Style shindig last night (April 18) and Kendra Wilkinson took some time to talk about what she finds attractive in a man.
The former "Girls Next Door" babe began, "What I think is hot is confidence…and is being in front of the eyes that matter to you most."
"I think 'hot' is being confident. At the end of the day that is all that matters--is what I wear in the bedroom," she added.
And Ms. Wilkinson would know- she's a former Playboy playmate, and she's got a hunky football stud hubby named Hank Baskett to come home to every night.
Photo Credit: Getty Images


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Tornados reschedule SAC twinbill with Carson-NewmanApril 12, 2011
Brevard, N.C. – The Brevard College softball team has postponed their South Atlantic Conference doubleheader on Monday, April 11th with Carson-Newman College. Their games with the Eagles have been rescheduled to Wednesday, April 13th at 2 p.m. in Jefferson City, Tennessee.
The Tornados doubleheader with the Belmont Abbey Crusaders on Tuesday, April 12th, has been cancelled due to the threat of bad weather in Bemlont, North Carolina on Tuesday afternoon.


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Health Care Businesses For Sale in Champlin MN
Searched the DealStream database for Health Care Businesses For Sale in Champlin MN and found no matches. Expanded the search to nearby areas and found the following matches.
Minnesota Home Health for Sale #471582 posted Jul 6, 2017
- Minneapolis, Minnesota, United States
- $749,000
Minnesota Home Health for Sale. $1,537,000 Total Revenue 2016 Twin Cities Area Home Health. Medicare Certified Medicaid Accredited 5% Medicare Payor 25% Medicaid Payor State Approved Fully Staffed 225 Census Can Service 50 Mile Radius of Twin Cities, Minnesota Asking Price: $749K Reference...
Well-Estab. Veterinary Practice Near the Twin Cities #470520 posted Jun 16, 2017
- Saint Paul, Minnesota, United States
- $120,000
Located just outside the Twin Cities metro area, this well-equipped and well-established Small Animal Veterinary practice has a prime location on a highly-trafficked street. The stand-alone building has 3,000 sq. ft. of space and is outfitted with an impressive array of equipment. The practice has...
Unique Home Health (Personal Care) Business #466342 posted Apr 11, 2017
- Saint Paul, Minnesota, United States
- $550,000
Excellent acquisition opportunity available for another home health (personal care) company to add revenues and benefit from economies of scale. The business has earned a reputation for providing excellent personal care services to their clients. In business for over 30 years with consistent revenues...
Beautiful 24 Bed Assisted Living Complex #465335 posted Mar 25, 2017
- Saint Paul, Minnesota, United States
- $1,500,000
Located in Northern Minnesota, this well-established assisted living facility is well known for providing extremely high quality care. Featuring a total of 24 beds and a waiting list spanning several years, this is a terrific opportunity to get into the lucrative and growing senior care business. ...
Price Just Reduced to $119k on a Fully Equipped Op #456418 posted Oct 22, 2016
- Saint Paul, Minnesota, United States
- $119,000
Due to a serious health condition, the owner/OD of this long-standing Optometry practice is looking for a buyer that can enter into an agreement to purchase the practice by the end of this year. As an incentive, the asking price has been reduced to $119k for a buyer that can make that happen. ...
High Growth, Franchised In-Home Senior Care #450950 posted Aug 4, 2016
- Saint Paul, Minnesota, United States
- $100,000
This franchise has been growing steadily over the past few years with cashflow Doubling every year since 2013. A fantastic opportunity to get into the exploding in-home senior care space with a significantly reduced franchise fee! The franchisor will train a new buyer and the seller will assist in...
Price Reduced to $299k for an Optometry Practice #449118 posted Jul 9, 2016
- Saint Paul, Minnesota, United States
- $299,000
This well-established Optometry practice operates out of a beautiful, multi-tenant "Class A" office building in a highly desirable West Metro location. The practice is a primary care Optometric clinic that provides comprehensive eye care and treats glaucoma, dry eye, ARMD, other forms of eye disease,...
Price Reduced to $795k/Fully Equipped Optometry #407570 posted May 20, 2016
- Saint Paul, Minnesota, United States
- $795,000
This well-established Optometry practice is fully equipped with state of the art equipment, making it one of the most complete Optometric facilities in the Twin Cities. It is situated inside of the 494/694 loop in a highly visible location with substantial drive by traffic and ample, convenient parking. ...
Turnkey Optometry Practice for Sale in Northern MN #407500 posted May 19, 2016
- Saint Paul, Minnesota, United States
- $450,000
This practice is the sole Optometry practice in a lovely Northern MN town. The owner/OD has been providing comprehensive eye exams as well as diagnosing and treating various eye diseases for nearly 20 years. The practice carries a wide assortment of stylish, well-known brands of frames and sunglasses...
Family Practice Saint Paul #382145 posted May 27, 2015
- Saint Paul, Minnesota, United States
- Between $500,000 and $1,000,000
2 person family medicine practice. Est. 32 years. In upscale St. Paul neighborhood with good reputation. Owners want to retire. Collections 1.15M. Net to providers 430K. Building also available separately or to lease.
Northern MN Optometry Practice/ Price Reduced #372544 posted Jan 15, 2015
- Saint Paul, Minnesota, United States
- $329,000
This is a great opportunity for an O.D. to practice in a lovely part of Northern Minnesota where the quality of life is high and cost of living is low. The practice has had an annual owner's cash flow of $200k in each of the past 4 years with the owner/O.D. working no more than 4 days a week. This...
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Trilobite discovery shows the unique calcite eye lens of at least one species 'dalmanitina socialis' had a central bulge which had the optic properties of a 'bifocal lens' according to Gal, Horvath, Clarkson and Haiman, Vision Research (2000), Vol 40, Issue 7, pps.843-53.
Editorial Comment: The more we find out about the optic systems of creatures which the evolutionist treats as primitive, the more amazingly complex and modern they appear. These eyes are probably without any known evolutionary ancestry. (Ref. Trilobites, optics, evolution)


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I have since a couple of years used the XBMC database directly (through ODBC) to
- get nice formatted lists of movies and TV series
- finding doubles
- moving whole TV-series to other locations without loosing all the user data (watched, time when watched, etc)
- and much more.
I have noticed a couple of things with it.
- the database is not normalized so it has to be some problems for you to use it when programming, compared to a normailzed database.
- It has structure problems. For instance it does have the information about the id of the movie connected to the users data (watched/unwatched, when it was imported and watched). Then it deletes the private information when moving or deleting and then take the movie back after a while.
- a lot of "Cxx" field names
- a lot of wrong types for fields (char when it is supposed to be numeric and such, for instance the season and episode field (C11,C12?)
- and also here, much more
I have been waiting for Eden to see if you were going to change the structure of the database but now I can see that it is not done any major changes.
My question is simply if you are interested in me rewriting the whole database and (among a lot of other things) make it normalized? That is having the right tables, the right connections between the tables and the right fields in the right tables.
I will not go into much details if you are not interested but some of the differences would be:
- to separate movie/series data with the users information about movies/series. That would make it possible to move movies/series without loosing the users information. Show that movies are offline (maybe make profiles obsolete with a toggle for showing/not showing offline movies/series because then you can have sources for all the places you are with your laptop (if not using a centralized database)). Delete a whole TV-series and after some time you want to see it again and then put it back (maybe in another format) and still be able to have it marked as watched and also see when it was watched that last time. And even if you mark it unwatched (because you want to see it again) the information of when it was last seen will still be there.
- when it is normalized it is much easier and more natural to use the database when coding.
- better naming of fields
- all fields will be with the right type so they don't have to be converted before use.
(I think) there is a smart way of creating new versions of a database in XBMC. That is one of the reason why a total rewrite wouldn't be that hard to implement. And it is often impossible to do all the coding changes in the same time but that is also easy to use. For all the reading and hopefully a lot of the writing (depending on how SQLlite/MySQL is handling that, and that I don't know) of the database it is possible to use SQL views that looks exactly as the old database looks like, making the transformations easy and the code-changing a process that can be done whenever all the different programmers have the time to do it.
This is not any criticism of how the database looks right now. It is very common that the database gets like this after some years of using when building a lot of new features onto the first version.
In my profession I have been working with MS SQL Server for over 15 years.


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If you love cats but suffer from allergies, don't be discouraged. Here are a few breeds that won't send you running for Benadryl.
Believe it or not, cat hair isn't the issue. The allergies are caused by a protein found in cats' saliva, urine and dander, according to WebMD. No breed is completely hypoallergenic, but different cats produce this protein in varying amounts. Here you will find a few breeds that have been classified as hypoallergenic by PetBreeds.


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On Campus
For information about on-campus housing, visit the Jefferson Housing and Residence Life web site at http://www.jefferson.edu/housing/options/short_term.html. Unfortunately, our office cannot arrange housing for you. Your department must request a reservation if you would like on-campus housing.
Off Campus
Information about off-campus housing can be found through any of the following web sites:
- Off Campus Listings Bulleting Board
- Apartments.com
- Craigslist (Philadelphia Apartments & Housing)
- International House of Philadelphia (on University of Pennsylvania campus)
- Rent.com


text:
cooran wrote:And for those Americans who said they would move to Australia if Obama was re-elected .... there is something you should know:
Australia has Universal Health Care,
Compulsory Voting,
No Guns,
No Death Penalty,
Pro-choice when it comes to Contraception,
Openly Gay Politicians and Judges,
Evolution is Taught in all Schools,
And our Female Prime Minister is an Unmarried Atheist.
Be sure to Declare Your Pitchforks at Tullamarine Airport.
with metta
I have just found this on http://www.abc.net.au/technology/articles/2012/11/08/3628527.htm. Maybe that's where you found it, too?
But other stuff there deserves an airing too:
... and "Obama Tweeted the most Retweeted Tweet ever (https://twitter.com/BarackObama/status/266031293945503744) - knocking Justin Bieber off his perch and thereby restoring some faith in humanity."