text:
This one is splintering the right isn't it. Bolton sees it just the opposite.
Bolton seem to be a RINO.
I think it is a case of if you are for big government, Right or Left, you hate this guy. If you are for little government, Right or Left, you support this guy.
The Left, although not monolithic, pretty much support big government - so they are nearly unanimous in their hatred for Snowden. The Right is more split between its big government and little government supporters.
Bolton is wrong on this one.
The government should not be collecting my numbers, emails, phone calls, surfing, and their contents.
But they are. There is no justification for it. I have done nothing wrong.
The only possible explanation is that they are keeping records against people for the day when any particular person might be a threat.
Geez. Who cares what Michael Bolton thinks about this? Shut up and sing, Bolton!
It is splintering the left also....maybe not the talking heads so much but go read the posts after every article posted at each news organization....the masses are not happy campers with either party.


text:
129 N Van Rensselaer St PO Box 27
Rensselaer, IN 47978
Jordan Balvich, D.M.D. is proud to be a trusted Rensselaer area Zoom!® teeth whitening dentist. Our patients love the short amount of time the tooth whitening treatment takes as much as they love the results.
You'll be able to relax in our comfortable surroundings. In just about an hour, you'll have a sparkling white smile!
Don't let untreated dental problems get you down. Rebuild your confidence with a beautiful new smile. From simple treatments that make a big difference, to more extensive work, unveiling the smile you were meant to have can have profound life-changing effects. Serving the Rensselaer, IN area and offering cosmetic dentistry, Dr. Balvich can help you develop an affordable treatment plan you'll feel good about.
There are a variety of payment options available to our valued patients. Jordan J. Balvich, DMD, PC accepts debit cards, credit cards, checks and cash, and even offers convenient monthly payment plans for those who qualify. Call our Rensselaer area dental office today to discuss the right financial options for you.
If you suffer from dental anxiety in the Rensselaer area, Dr. Balvich can help. We offer several options that can make your dental treatments pleasant and stress-free. Our team at Jordan J. Balvich, DMD, PC is trained to help you feel comfortable. You'll be able to feel relaxed while receiving the friendly, professional oral care you deserve.
Dr. Balvich has extensive experience providing gingivitis treatments in the Rensselaer area. Bleeding gums can lead to more serious health problems if not treated promptly.
Our friendly, professional team will evaluate your needs and design a personal treatment plan. We'll help you have healthy gums and teeth for life.
Grafe, Brent R D.D.S.
2709 Washington Ave # 1
Evansville, IN, 47714-2450
Hamilton County Endodontics
14757 Oak Rd # 400
Carmel, IN, 46033-8179
Anderson, Donald D.D.S.
3105 E 98th St # 130
Indianapolis, IN, 46280-2006
Kokomo Endodontics
112 E Alto Rd
Kokomo, IN, 46902-3601
Hollywood's most fortunate faces often rely on the skills of their cosmetic dentist in ways that may surprise you.
Dustin Hoffman and Meryl Streep have perfectly nice teeth on their own. But, to meet the challenges of demanding roles in films, both used cosmetic dental appliances (dentures) to change the looks of their natural teeth.
Since women have smaller, rounder teeth, Hoffman, (who played a woman in "Tootsie") used a partial denture to make his teeth look more feminine.
To suggest the brutality of life in a concentration camp, Meryl Streep's teeth and mouth were altered for her role as Sophie Zawistowska, the poignant heroine of "Sophie's Choice."
More commonly, actors see their cosmetic dentists long before they get in front of the camera. In an image-conscious industry, it's no secret the camera is a harsh critic. And a mouth with missing teeth, or a mouthful of stained or broken teeth never helped an actor's image - or the image of a banker, doctor or secretary for that matter.
Few of us face a camera at work every day. But we all face an audience and it's the same audience that goes to the movies. The movies tell us successful people look good, failures don't. Ugly teeth can tell the tale.
Hollywood uses dentistry to make villains out of heroes - you too can use cosmetic dentistry to get a "winning" smile. Restorative dentistry is available to anyone who wants to look better.
Techniques such as dental veneers and tooth bonding have joined the traditional process of dental caps as ways to improve smiles. Orthodontic braces aren't just for children anymore, they're also available to adults who want straight teeth. In some cases, you can opt for teeth bleaching, or have your teeth lengthened or sculpted. If you think you would benefit from these treatments, ask your cosmetic dentist. Not all the new techniques will work for everyone, but he or she will be glad to discuss the best plan for your smile.
With today's new cosmetic dental techniques, you can cast yourself successfully in a competitive world with an award-winning smile.
People often assume that once they have a crown placed on a tooth, it will last the rest of their lives. Although dental crowns may last for a very long time, it is not correct to say that they will last forever.
This is one of the most difficult questions asked of a dentist because, in essence, no one can be sure. However, there are a few resources available that can give us an idea of how long your dental crowns may last.
One resource is insurance companies. Even though the dental insurance company should not dictate the type of treatment that should be done, they give some insight into the matter. Insurance companies will pay for a new crown on the same tooth after five years. So, in essence, they believe that dental crowns will last at least five years. Another resource available is the dental literature. The dental research has some extreme variability in this area. It provides information that ranges from a 20% failure rate in 3 years all the way to a 3% failure rate in 23 years. So, which is correct? The answer actually has to be looked at on a case-by-case basis. To do this, we need to look at why dental crowns have to be replaced.
There are many reasons why dental crowns need to be redone. Fracture is one example. The fracture incidence of dental crowns can be related to either the type of restoration (gold, metal ceramic, all-ceramic) or where the tooth restoration is placed in the mouth (anterior vs. posterior). Another risk factor is if a person has a grinding habit at night. A crown will have a higher risk of fracture in a mouth that applies more forces to the teeth by grinding than in one that does not have any incidence of grinding.
Decay can be another reason why dental crowns need to be replaced. Just because a tooth receives a crown does not mean it is less prone to decay. In fact, because it has more areas that may trap plaque, it needs to be cleaned as well, if not more meticulously, during home care.
Esthetics can be another reason why dental crowns are replaced. Esthetics is one of the main reasons people choose to have a new crown made in the anterior part of the mouth. This occurs because as we get older, our teeth will change color and progressively get darker while the tooth with the crown will stay the same color as the day it was placed. A discrepancy between the color of the teeth will become more evident over time. In addition to color, esthetic changes in the position of the gum tissue over time can also affect the look of the crown.
Which one of these areas may be a factor in a patient's mouth is uncertain. An idea can be obtained by evaluating the reason a crown was needed in the first place (such as a fractured tooth, decay, etc.). In conclusion, it is unclear how long dental crowns will last. Their life span may be anywhere from a few years all the way to 20 years and beyond. A lot of it will depend on the need for the crown in the first place and how well the mouth is taken care of after the crown is placed.
By Greggory Kinzer, DDS, MSD

subset: commoncrawl


text:
Certificates of Appreciation were given to the original partners as follows:
Site Highlights
More photos to share and enjoy.
Linda Ulland Memorial Gardens (LUMG) is an environmental, educational, natural destination for all ages.
Like the Lights? Eat a Brat!
A Grand Opening of Linda Ulland Memorial Gardens
By Paul Boblett, Editor
'...While it was created in part to help fund biking shoulders, the byway has become more than that. It's a unifying force among several separate but neighboring communities."
The historic DNR fire tower on CSAH 11 will be closed to climbers until structural repairs have been assessed and implemented.


text:
Issue 181: Queerkateers Quasemble: Marvel's The Avengers – Movie Wrap-Up
Our Avengers coverage continues with as we put a condom on Marvel's The Avengers in our movie Wrap-Up. Join StevieD, The Scavenger, Saro Spice, TonyL (with a brief cameo from PattyBoom). What were our favorite moments, our least favorite moments and the "where the heck was that moment?". Learn which "Growing Pains" star has a good agent (besides Leonardo DiCaprio that is).
Spoilers ahoy so if you haven't already seen one of the top grossing films of all time, it means you've been stuck on a mountain top away from a theatre. And if that's the case, focus on getting off the mountain!


text:
[Plugin: My Xbox Profile ] 3.01?
Anyone have any idea if this works in 3.01. I guess I could try but didn't want to waste the time if someone already has.
Viewing 1 replies (of 1 total)
Viewing 1 replies (of 1 total)
- The topic '[Plugin: My Xbox Profile ] 3.01?' is closed to new replies.


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Daniel Naber <daniel.na...@t-online.de>
|Re: Normalized Scoring -- was RE: idf and explain(), was Re: Search and Scoring
|Thu, 21 Oct 2004 21:35:27 GMT
On Thursday 21 October 2004 23:03, Doug Cutting wrote: > Idf's are already computed globally across all indexes. Tf's are local > to the document. In short, scores from a MultiSearcher are the same as > when searching an IndexReader with the same documents. That doesn't seem to be the case in the attached test -- am I using MultiSearcher in the wrong way or what might be the problem? The output of the attached test is: 1+2 searched with Multisearcher: two blah three score=0.70273256 one blah three score=0.35615897 one foo three score=0.35615897 one foobar three score=0.35615897 1+2 indexed together: one blah three score=0.5911608 one foo three score=0.5911608 one foobar three score=0.5911608 two blah three score=0.5911608 -- http://www.danielnaber.de
|View raw message


text:
Major City Developments
Major City Developments
Southampton has a multitude of city developments planned for the next 20 years; some of which are already charging 'full steam ahead' (see our City Centre Master Plan page for details). While our visionary plans bubble away, enthusiasm for these projects is already palpable among the local community of residents and businesses, with much interest being shown from investors further afield. After all, many of our plans have our residents' and businesses' interests at heart, and will ensure economic, cultural and social development in the city.
Once realised, these projects will consolidate our city as a leading, low-carbon hub for business and leisure. We hope you will be as excited as we are about the extensive urban developments outlined in our City Centre Master Plan, as well as our vision for comprehensive regeneration and improvement work in other parts of the city. We are in no doubt that our bold transformations will attract more and more people to Southampton over the coming years.
Please click here to view our plans for the city and to get a feel for future Southampton.
Scroll through the images below for a taster of some of our recent developments.
Southampton Central Police headquarters
IKEA Southampton
Charlotte Place redevelopment
Splash, Ocean Village
Redevelopment of the south entrance and atrium to Central Station
Redevelopment of the south entrance to Central Station
Admiral's Quay, Ocean Village
SeaCity Museum
Empire View
- Previous
- Next


text:
This song. OMG. It's so horrendously evocative and haunting. It's like that horrible car accident of the side of the road that I have to slow down to see. It reminds me so much of the dark side of my youth. The obvious slight variations in the speed of the record make her sound like she is singing incredibly flat. It also makes me feel like I took a handful of qualudes. When I listen, I cringe…but I have to listen.
The should totally use this as background music during a serial killer murder scene on CSI.


text:
|Home | About | Journals | Submit | Contact Us | Français
Abnormal activities of histone lysine demethylases (KDMs) and lysine deacetylases (HDACs) are associated with aberrant gene expression in breast cancer development. However, the precise molecular mechanisms underlying the crosstalk between KDMs and HDACs in chromatin remodeling and regulation of gene transcription are still elusive. In this study, we showed that treatment of human breast cancer cells with inhibitors targeting the zinc cofactor dependent class I/II HDAC, but not NAD+ dependent class III HDAC, led to significant increase of H3K4me2 which is a specific substrate of histone lysine-specific demethylase 1 (LSD1) and a key chromatin mark promoting transcriptional activation. We also demonstrated that inhibition of LSD1 activity by a pharmacological inhibitor, pargyline, or siRNA resulted in increased acetylation of H3K9 (AcH3K9). However, siRNA knockdown of LSD2, a homolog of LSD1, failed to alter the level of AcH3K9, suggesting that LSD2 activity may not be functionally connected with HDAC activity. Combined treatment with LSD1 and HDAC inhibitors resulted in enhanced levels of H3K4me2 and AcH3K9, and exhibited synergistic growth inhibition of breast cancer cells. Finally, microarray screening identified a unique subset of genes whose expression was significantly changed by combination treatment with inhibitors of LSD1 and HDAC. Our study suggests that LSD1 intimately interacts with histone deacetylases in human breast cancer cells. Inhibition of histone demethylation and deacetylation exhibits cooperation and synergy in regulating gene expression and growth inhibition, and may represent a promising and novel approach for epigenetic therapy of breast cancer.
In cancer, histone deacetylases (HDACs) may have abnormally high activity and thus contribute to hypoacetylation and the aberrant silencing of genes [1]. A number of synthetic HDAC inhibitors have been rationally developed. Among the most interesting inhibitors are those that have been designed to target primarily the zinc cofactor at the active site of class I/II HDACs [2, 3]. Some of the HDAC inhibitors have been examined for their ability to alter chromatin structure and re-express aberrantly silenced genes in breast cancer [4–7].
Another important protein that is responsible for abnormal chromatin remodeling and epigenetic silencing of genes is a recently identified histone lysine-specific demethylase 1 (LSD1). LSD1, also known as AOF2 or KDM1A, is the first identified FAD-dependent histone demethylase capable of specifically demethylating mono- and di-methylated lysine 4 of histone H3 (H3K4me1 and H3K4me2) [8, 9]. LSD1 has been typically found in association with HDAC1/2, Co-REST, BHC80, and BRAF35 [8]. Several recent studies have established LSD1 as an important link to the development and progression of cancer and provide a rationale for developing LSD1 inhibitors as a means for therapeutic intervention [10–12]. In our recent studies, we have shown that specific polyamine analogs function as potent inhibitors of LSD1, leading to re-expression of several aberrantly silenced tumor suppressor genes [13–15]. Recently, a second mammalian FAD dependent histone demethylase, LSD2 (also known as AOF1 or KDM1B), has been identified [16–18]. Amino acid sequence analysis shows that LSD1 and LSD2 share 33% overall identity in the amine oxidase domain. However, little is known about the actual biological function of LSD2 in cancer.
Despite the promising results produced by HDAC inhibitors in preclinical studies, lack of specificity may limit the clinical use of these compounds in cancer treatment. One of the major hurdles in predicting the efficacy of HDAC inhibitors is identifying potential selectivity for one or more epigenetic protein complexes and determining the ultimate effect on gene expression. To overcome these obstacles, it is necessary to better explore the epigenetic mechanisms underlying the activity of HDAC inhibitor and seek a more effective and less toxic therapeutic approach by combining HDAC inhibitors with other epi-drugs that can effectively target multiple epigenetic entities. In this study, we define in depth the mechanisms of functional crosstalk between histone demethylase and deacetylase in chromatin remodeling and gene transcription in breast cancer cells.
SAHA was purchased from Cayman Chemical (Ann Arbor, MI). TSA and pargyline were obtained from Sigma–Aldrich (St. Louis, MO). MS-275, LBH-589, and PXD-101 were purchased from Selleck Chemicals (Houston, TX). Human breast carcinoma cells MDA-MB-231 were maintained in DMEM medium and MDA-MB-468 cells were maintained in IMEM medium (5% FBS).
Whole and nuclear proteins were extracted as previously described [19, 20]. Proteins were fractionated on SDSPAGE gels and transferred onto PVDF membranes. Primary antibodies against H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, Acetyl H3, Acetyl H3K9, LSD1, HDAC1, HDAC2, and CoREST were from Millipore. The H3 antibody for normalization was purchased from Abcam (Cambridge, MA).
Pre-designed and validated LSD1 siRNA oligonucleotides and non-targeting siRNA (scramble) were purchased from Ambion (Carlsbad, California). Transient transfections were performed using siPORT™ transfection agent (Ambion). After 48 h exposure to a 50 nM LSD1 siRNA, cells were harvested and the lysate was analyzed for LSD1 protein expression. siRNA targeting LSD2 mRNA and a non-targeting scramble oligonucleotides (targeting sequences, LSD2-siRNA: 5-AAGACATTCAAGGAACCGTCT, Scramble: 5-AACTTGCTATGAGAACAAATT) were synthesized using Silencer® siRNAconstruction kit (Ambion). Transient transfection was performed with LipofectAMINE 2000 reagent (Invitrogen). After 24 h of exposure to 70 nM LSD2 siRNA or scramble oligonucleotides, total RNA was extracted using the TRIzol reagent (Invitrogen).
The TaqMan® Gene Expression Assays (Applied Biosystems) were performed to quantify mRNA expression of LSD2, NR4A1, NR4A3, PCDH1, BIK, and RGS16 genes with actin as an internal control.
MTT assays were performed using the method as previously described [19]. The median effects (IC50) were determined by CalcuSyn software (Biosoft, Cambridge, UK). The Chou-Talalay median effect/combination index (CI) model was used to determine synergy, additivity, or antagonism of combination treatments [21]. Briefly, cells were treated with each agent individually at its IC50 concentration or fixed fractions of the IC50 concentrations [22]. The agents were also combined in these same dose-fixed ratios to determine the combination index (CI). Synergy was defined as any CI < 1, additivity as CI = 1, and antagonism as any CI > 1.
Cells were treated with 5 µM SAHA, 2.5 mM pargyline or in combination for 24 h. Total RNA samples from three independent biological replicates (n = 3) were extracted using Qiagen RNeasy kit (Qiagen, Valencia, CA). The array study was performed using Affymetrix GeneChip U133A 2.0 array platform, which contains 20,928 probes representing all functionally characterized genes in the human genome. The data were processed as RMA files (Affymetrix Robust Multi-Array Average) in which the raw intensity data were background corrected, log2 transformed, and then quantile normalized according to Affymetrix recommendations.
The Student's t-test was used to determine the statistical differences between various experimental and control groups. Microarray statistical tests were performed using Significance Analysis of Microarrays software (SAM version 3.09c), which is designed to reduce the risk of Type 1 errors due to multiple testing [23].
To determine whether histone lysine methylation is functionally linked to activity of histone deacetylase in breast cancer, global nuclear H3K4 methylation was examined after exposure of human breast cancer cells to a variety of HDAC inhibitors. We first tested several clinically relevant inhibitors that have been designed to target primarily the zinc cofactor at the active site of the class I/II HDACs. These inhibitors include hydroxamic acid derivatives SAHA (Vorinostat), TSA (Trichostatin A), LBH589 (Panobinostat), and PXD-101(Belinostat), and a benzamide analog MS-275 (Entinostat). In both MDA-MB-231 and MDA-MB-468 cell lines, exposure to these compounds produced significant global increase of nuclear H3K4me2, which is the specific substrate of LSD1 (Fig. 1a). The enhanced level of histone methylation by HDAC inhibitors parallels the increase of acetylation of histone 3, suggesting that LSD1 is an important epigenetic target of HDAC inhibitors and its activity is intimately associated with HDAC activity. Recently, another class of HDACs, the class III NAD+-dependent sirtuins, has received increasing attention as a potential epigenetic target [24]. To evaluate if inhibition of zinc-independent sirtuins could also affect the LSD1 activity, we applied a pharmacologic approach using splitomicin (SPT) and nicotinamide (NIA), two specific inhibitors against sir2-dependent class III HDACs. Neither of these sirtuin inhibitors altered H3K4me2 level in breast cancer cells (Fig. 1b).
A co-immunoprecipitation study showed that the physical interaction between LSD1 and HDAC1 remained intact under SAHA treatment (Fig. S1). In addition, LSD1 mRNA and protein levels were not altered by increasing concentration of SAHA treatment (Fig. S2). This suggests that inhibition of LSD1 activity by HDAC inhibitors likely occurs through disruption of the functional link between the two enzymes rather than direct interruption of their physical interaction or suppression of the mRNA and protein levels of LSD1.
To probe precisely the role of LSD1 in regulation of HDAC activity, MDA-MB-231 and MDA-MB-468 cells were treated with an LSD1 inhibitor, pargyline. MTT study indicates a dose-dependent growth inhibition by pargyline (Fig. 2a). Exposure of MDA-MB-231 cells to pargyline produced significant increase of H3K4me2 and H3K4me1 at concentrations ≥0.5 mM (Fig. 2b). Treatment with pargyline also significantly increased pan acetylation of H3 at concentrations ≥2.5 mM (Fig. 2b).
The exposure of MDA-MB-231 cells to pargyline did not change the expression of LSD1, CoREST, or HDAC1/2, but led to a remarkable increase of H3K4me2 and AcH3K9 (Fig. 2c). Similar results were seen for SAHA treatment (Fig. 2c), suggesting an intimate functional link between LSD1 and HDAC1/2. Neither pargyline nor SAHA increased H3K4me3 or two important repressive marks, H3K9me2 and H3K27me2, suggesting that the inhibition of LSD1 or HDAC activities does not affect the activity of another class of histone demethylase family, the JmjC containing histone demethylases.
Exposure of MDA-MB-231 cells to LSD1-targeting siRNA resulted in a significant decrease in LSD1 protein without affecting the protein expression of CoREST, HDAC1/2 (Fig. 3a). Similar to pharmacological inhibition, LSD1 siRNA treatment in MDA-MB-231 cells led to significantly increased nuclear levels of H3K4me2 and AcH3K9 (Fig. 3b). This result strengthens the evidence that LSD1 and HDAC activities are indeed functionally associated.
To understand whether LSD2, a new member of the FAD dependent histone demethylase family, in concert with LSD1, interacts with histone deacetylase in human breast cancer cells, we used siRNA approach to knock down LSD2 mRNA expression. Specific LSD2 siRNA oligonucleotides suppressed >90% LSD2 mRNA expression (Fig. 3c) and led to increase of H3K4me2 (Fig. 3d), but failed to alter the global level of AcH3K9 in MDAMB-231 cells (Fig. 3d). These results suggest that LSD2 possesses histone demethylase activity in breast cancer cells, but, unlike LSD1, the activity of LSD2 may not be functionally associated with HDAC activity.
To determine whether combination treatment with LSD1 and HDAC inhibitors could lead to a synergistic effect in chromatin remodeling, we examined the nuclear levels of H3K4me2 and AcH3K9 in MDA-MB-231 cells treated with pargyline or SAHA alone or in combination. Combined treatment resulted in a remarkable increase of H3K4me2 and AcH3K9 (Fig. 4a). To examine whether such chromatin modification also translates to enhanced therapeutic efficacy of the drugs, MDA-MB-231 cells were treated with pargyline and HDAC inhibitors alone or simultaneously for 48 h. The combination index (CI) values were evaluated by using the CalcuSyn program. At very low dose combination (fractional growth inhibition, Fa = 0.9), synergistic growth inhibition (CI < 1) was observed between pargyline and HDAC inhibitors SAHA, TSA, MS-275, and LBH-589 (Fig. 4b). At median or higher dose combination (Fa = 0.5 or 0.75), pargyline exhibited synergy with all the HDAC inhibitors tested (CI < 1) (Fig. 4b). These results indicate that treatment with LSD1 and HDAC inhibitors significantly enhanced growth inhibition when used in combination in human breast cancer cells.
The synergistic effect in growth inhibition by combined treatment with LSD1 and HDAC inhibitors suggests that simultaneous inhibition of both enzymes may have a more profound effect on gene transcription than inhibition of either enzyme function alone. To test this hypothesis, we performed a genome-wide microarray screen of MDA-MB-231 cells to define a comprehensive profile of genes whose expression is altered by combination treatment with LSD1 and HDAC inhibitors. Microarray results showed that expression of a total of 671 genes was changed by 1.5-fold or greater by SAHA alone (416 genes up-regulated and 255 genes down-regulated, P < 0.01) and expression of 34 genes was altered by 1.5-fold or greater following pargyline treatment alone (32 genes up-regulated and 2 genes down-regulated, P < 0.01). Further analysis showed that 932 genes exhibited significant change in their mRNA levels after exposure to both drugs (593 genes up-regulated and 339 genes down-regulated with 1.5-fold or greater, P < 0.01) (Fig. 5a). Among these affected genes, we identified a unique set of 241 genes whose expression was exclusively induced by combination therapy (Fig. 5a), 81 of which displayed a 1.5-fold or greater induction in expression (Fig. 5b). The detailed annotation for these genes is shown in Table 1. The array study also identifies a subset of 132 genes, whose expression was exclusively down-regulated by combination (Fig. 5a). The list of down-regulated genes by SAHA/pargyline with 1.5-fold or greater change (P < 0.01) is shown and annotated in Fig. 5c and Table 2.
To validate the microarray results, five genes whose expression levels were uniquely induced by combination treatment were selected for evaluation by qPCR because of their potential to play an important role in breast tumorigenesis and therapeutic response. These genes include nuclear receptor subfamily 4, group A, member 1 and 3 (NR4A1, NR4A3), proto-cadherin 1 (PCDH1), BCL2-interacting killer (BIK),and regulator of G-protein signaling 16 (RGS16). The same set of mRNAs used for microarray analysis was reverse transcribed and the qPCR results were consistent with microarray results that the combination resulted in a striking synergistic increase in expression of all the selected genes (Fig. 6).
We also used DAVID Bioinformatics Resources 6.7 (NIAID/NIH) to categorize the genes into biological groups based on functional similarity. Identified genes have roles in a wide range of cellular functions including cell proliferation and death, cell signaling, transcription regulation, cellular movement, metabolic processes, etc. (Supplementary Table S1).
LSD1 has been proposed to demethylate its histone substrate that requires the intimate collaboration between LSD1 and HDAC1/2 [9, 25, 26]. Our current study demonstrated that treatment with zinc dependent class I/II HDAC inhibitors remarkably diminished the activity of LSD1 in breast cancer cells, suggesting that LSD1 is an important downstream target of specific HDAC inhibitors in breast cancer. This is in contrast to a lack of measurable increase of H3K4me2 after treatment with NAD+ dependent class III HDAC inhibitors, splitomicin, and nicotinamide, suggesting the specific interplay between LSD1 and class I/II HDACs, but not class III HDACs. We also demonstrated that either pharmacological inhibition or knockdown of LSD1 expression by siRNA enhanced the acetylation of H3K9, a critical mark for gene activation. These observations indicate that histone demethylation is an important component of the activity of HDAC inhibitors in breast cancer cells.
We showed that LSD2 possesses the activity to demethylate H3K4 in breast cancer cells. This clearly suggests the existence of a more sophisticated FAD-dependent histone demethylase family whose members play a role in chromatin remodeling and transcription regulation in breast cancer. Knockdown of LSD2 did not lead to enhanced lysine acetylation, indicating that, despite structural and catalytic similarities, LSD2 is likely to be part of chromatin-remodeling complexes different from those involving LSD1 and HDACs.
Previous studies have shown the synergistic effects of the combination of DNA methyltransferase and HDAC inhibitors in re-expressing epigenetically silenced genes in cancer cells and leading to clinical responses in patients with leukemia [27, 28]. Our study provides evidence that the HDAC inhibitor and LSD1 inhibitor cooperate to increase both histone methylation and acetylation marks in breast cancer cells, and lead to significant synergy in growth inhibition when used in combination. Through microarray screening, we identified a unique subset of genes whose expression was significantly up-regulated by combined inhibition of LSD1 and HDACs. The enhanced transcriptional activation of these genes appears to lie in the collaboration between the activity of histone methylation and acetylation which are two critical components of transcriptional repressor complexes. Importantly, the genes identified are extensively involved in regulation of cell proliferation, cell signaling, transcription regulation, cellular movement, nucleosomal assembly, and metabolic processes in breast cancer. For example, NR4A1 and NR4A3 act as critical nuclear transcription factors, translocations of which from the nucleus to mitochondria induce apoptosis and reduce migration in breast cancer cells [29, 30]. The integral membrane protein PCDH1 mediates cell– cell adhesion. The epigenetic silencing of PCDH1 has been reported to be associated with cancer-specific differentially methylated regions (DMRs) in breast cancer [31]. RGS16 plays a significant role in G protein signaling and pathways and the activity of RGS16 may inhibit breast cancer cell growth by mitigating phosphatidylinositol 3-kinase signaling [32]. BIK is known to interact with BCL2 in order to enhance programed cell death. Enhanced level of BIK has been reported in apoptosis-inducing reagent treated breast cancer cells [33, 34]. The findings from the array study have added new candidate genes to the existing panel of markers to assess the epigenetic and biological consequences of targeting histone demethylation and deacetylation in breast cancer. The protein expression and activity of the genes modulated by LSD1 and HDAC inhibitor induced chromatin remodeling and growth inhibition should be investigated further. It is anticipated that such studies will identify novel therapeutic targets whose expression may be manipulated to specifically hinder breast cancer cells.
This work was funded in part by NIH grant CA88843 and the Breast Cancer Research Foundation.
Electronic supplementary material The online version of this article (doi:10.1007/s10549-011-1480-8) contains supplementary material, which is available to authorized users.
Yi Huang, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA. Department of Pharmacology & Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Shauna N. Vasilatos, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA.
Lamia Boric, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA.
Patrick G. Shaw, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA. Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA.
Nancy E. Davidson, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA. Department of Pharmacology & Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
LifeChange International, established in 2001, is a non-profit organization whose purpose is to transform the lives and living conditions of people around the world.
For many years LifeChange has worked in Uganda building schools; supporting Dwelling Places, a children's home; providing for the education of thousands of children; assisting in community social needs; buying land for farming; establishing youth trade education programs (such as carpentry, tailoring, computer technology) and building dormatories. We are currently looking to raise an additional $25,000 that will house 64 Ugandan teens in our Youth Development Program.
In Mexico, LifeChange has participated in building many homes for people living in appalling conditions in the colonia's just outside of Juarez. In May 2011, LifeChange once again sent a team to build 3 homes in one week. At the same time, we took food, clothing and medical supplies to encourage and meet needs of those struggling in the surrounding community.
In the Philippines, much has happened in this past year. Ex-Marine Captain Jack Fairweather and his wife Tammy are rescuing children living on the streets and in appalling neglect, providing them a wonderful home. Currently around 20 children are living in three homes, receiving an education, food, a safe home, and medical care. In the coming months we are hoping to raise an additional $25,000 to purchase land and build an additional home for these children.
LifeChange is also involved in medical missional work, other health projects and education programs in the Middle East. In addition, we help support a family in Hungary who are engaged in marriage and family counseling throughout Europe. Here in the states we support a woman from Turkey who, after her husband was murdered for his faith as a Christian, moved to the States for her own safety and to share a message of forgiveness, grace and love to the Islamic community here.
To date, LifeChange has maintained a policy of sending 100% of all donations directly to the work donors specify. No portion is taken for publicity, promotion, offices and other support costs.


text:
It would be an enormous shot in the arm for the English women's game. With the formation of a National League five years ago, the quality of the football played by the best clubs has dramatically improved, but off the field, funding and support remain pitifully threadbare. The coaching, coverage, sponsorship and facilities available to women's clubs in Scandinavia and Germany are the stuff of dreams for English players who, in many cases, often have trouble getting time off work to play at all.
The national side, at least, are notably better equipped since the FA took them over three years ago, and in their group games they put paid to Portugal and Croatia without much difficulty. Italy, however, were too strong, leaving them to tackle Spain in this play-off. In the away leg, which kicked off at noon in the Andalucian heat of Montilla, near Cordoba, England were two behind before they came to terms with the conditions. In the second half, Croydon's Hope Powell pulled one back but the goalkeeper Rosa Serra made a string of fine saves to preserve Spain's advantage. Serra knows her opponents well; she played for Arsenal Ladies last season.
England's chances of beating her rest in no small part on the form of Wembley's Kelly Smith, a stunningly quick winger who, though not yet 18, has four goals in seven appearances. Somewhat more experienced, meanwhile, the Doncaster Belles captain Gillian Coultard will be hoping for a record 93rd cap in midfield.
Another name to watch is the goalkeeper Pauline Cope who with the Millwall Lionesses recorded a surprise 1-0 win over Coultard's Belles last Sunday to go top of the National League. The game was watched by barely 100 - but as Merseyside is a hotbed of women's football, with Liverpool, Everton and today's hosts Tranmere all having sides in the Women's National Division, the FA are confident they can do better and can surpass the 2,400 who turned out in Spain. With so much at stake, it would be a crying shame if they didn't.
- More about:
- Lions
- Southern Europe
- Spain
- UEFA Champions League


text:
Published on Wednesday, February 22, 2017
Consumer body Which? has suggested holidaymakers should consider flying from Southend airport instead of Luton after the former scored more highly in its recent comparison of the two London airports.
Which Travel? gave Southend five out of five stars in four out of five categories, which included the length of security queues and the qualify of the toilets. It awarded the airport four points for its shops and food outlets.
Luton on the other hand scored only one or two points in every category.
"Luton Airport may be 14 times bigger, but that's no excuse for such a poor customer experience," said Which Travel? "EasyJet serves both airport, so consider Southend for a cheap and considerably more cheerful trip."
Luton offers a faster connection to London, with the train journey taking 40 minutes instead of 53 minutes to Southend, but at £27.50 the train fare is £2.50 more expensive.
However, Southend currently offers flights to only 16 destinations whereas Luton serves more than 130. Luton handled 14.5 million passengers last year when fewer than one million used Southend.
Luton is in the process of expanding its passenger terminal and plans later this year to add more shops and restaurants, for which it received just one star from Which Travel?
....it's a 100 times WORSE!!!!!!! The place is a nightmare for travellers. LTN management make travelling through the airport as unpleasant and expensive as they can. AVOID LIKE THE PLAGUE!
By Peter Mannell, Wednesday, February 22, 2017
Whoever own LTN see it as a cash cow, every angle is pointed to profit. Convenience of pax is the last thing on their mind, seating is ripped out and replaced by yet another eatery, people sit on floors. They are expanding but I am not sure I would see it as an upgrade, just further to walk; Gate 1 is so far away that you can see planes landing at Stansted!
By William Staines, Wednesday, February 22, 2017
Heathrow loses its position as Europe's largest airport
Greek hotel sues British couple for 'fake' holiday sickness claim
Two UK airlines listed amongst world's worst
BA's head of customer experience has left
Jet2 stands by crew who removed hen party from flight
Lawyer questions why dog that bit Delta passenger was in the middle seat
Jon Platt fined and given 12-month conditional discharge
BA's head of customer experience has left
Content is king, but only if you do it right
Jet2 rumbles food-poisoning claimaints
BA slammed for plans to outsource calls centre jobs
Move scroll bar (above) left to right for more videos!
Delivering a seamless customer experience
Great Customer Experience can have a huge impact on a company's bottom line, and it starts with understanding your customer's expectations at each stage of the buying process.
You can book now your advertisement for via our online booking service or find out more.


text:
Our Story
About Us
Our Mission
Our Team
Ranger Training & Support
Our Partners
Six Tactical
Six Tactical is an organization like no other. Our ranks are comprised of combat veterans from the U.S. Special Operations community, scholars and business leaders. Our diverse cadre of instructors delivers world-class instruction based on case studies, analysis and careers of service in the Marine Force Reconnaissance Companies and Navy Seals Teams. Six Tactical's mission is to share the critical skills and core values that are the mainstays of the war-fighting profession.
University of Florida
The mission of the University of Florida's Maples Center is fourfold – Education, Research, Service, and Outreach. The Center utilizes its extensive expertise in forensic medicine to provide comprehensive services and innovative programs that relate to medicolegal death investigation.
ENODO Global
Enodo, an intelligence firm, utilizes a population-centric approach to understand complex social dynamics, forecast and mitigate community-based risks and develops engagement strategies that establish stakeholder relationships with communities.
The mission of DEEMI is to develop with ethics and integrity, systems that will save lives. Through education and training, and using Federal surplus military equipment, to encourage the spirit of volunteerism, and to facilitate the saving of lives in the State of Maine.
Last Days of Ivory
Last Days of Ivory is the PSA created by Director Kathryn Bigelow in an effort to end ivory-funded terrorism. The film aims to highlight the dangers brought forth from elephant poaching and to provide an actionable outlet for those who wish to stop species extinction and global terrorism.
Ivory for Elephants
Ivory For Elephants (IFE) leverages the power of media to inform people globally about the elephant crisis. Working with sponsors and media partners, IFE's goal is to increase awareness and raise funds to support non-profit organizations which protect elephant and rhinoceros in Africa and Asia.
Vestergaard is an international company dedicated to improving the health of vulnerable people, most of whom live in developing countries. Our game-changing solutions contribute to a healthier, more sustainable planet by fighting malaria, HIV/AIDS, diarrheal diseases and neglected tropical diseases.
VETPAW promotes capacity- and relationship building in East Africa, elevating the perception of the military at home and abroad while preventing the extinction of keystone species and ecosystems.
Contact us for more info on partnerships
Our Mission
The goal of Veterans Empowered to Protect African Wildlife is to help end the African poaching crisis and conserve endangered species. We do this by utilizing the unrivaled skills and experience of post-9/11 U.S. veterans to train park rangers and support their communities.
Stay Connected
Sign Up To Receive Our Newsletter
First Name
Last Name
Latest tweets
Tweets by @VETPAW
Follow VETPAW
Ways to Help


text:
Parking in IG3 8YB
Parking Space on Stapleford Avenue, IG2
Driveway on Avenue Terrace, IG2
Driveway on Whites Avenue, IG2
Parking Space on Church Road, IG2
Driveway on Church Road, IG2
Parking Space on Brook Road, IG2
Driveway on Avenue Terrace, IG2
Driveway on Avenue Terrace, IG2
Car Park on 910 Eastern Avenue, IG2
Information about parking in IG3 8YB
Looking for parking in IG3 8YB? You've come to the right place! Funnily enough, parking is what we do best - and we've got a sumptuous selection of 250 locations in IG3 8YB for you to choose from.
Our parking in IG3 8YB is up to 70% cheaper than what you'd usually pay, plus you can book your guaranteed space in advance right here - forget about feeding the parking meter.
No more fumbling for coins and no more circling the block looking for a space. As for parking tickets? You can wave goodbye to them too - if you overstay with us, you just pay for the extra time.
We're taking the pain out of parking for 1 million drivers across the UK. But don't just take our word for it - check out our latest customer reviews for IG3 8YB parking below:
"Nice and easy to find really nice people " — Driveway on Avenue Terrace, IG2
"A good experience. Easy to find and very handy for the tube station at Newbury Park. Many thanks, I would recommend the location, and use it again. " — Driveway on Avenue Terrace, IG2
Rent out a space
Got a spare driveway or garage in IG3 8YB? You can list your parking space for free with JustPark. It only takes a few minutes to get up and running, and you could be making money in no time. Find out more here.
Check out the JustPark app
You can now book any one of our 200,000+ JustPark spaces on the move with our mobile app - available for both iPhone and Android. View parking options, pay for a space and get directions - all with just a few taps of your phone.
Nearby Transport
- Upney Station 2.97 miles away
- East Ham Station 3.87 miles away
- Becontree Station 2.9 miles away
- Barking Station 3.14 miles away
- Dagenham Dock Station 2.95 miles away
- Woodgrange Park Station 3.59 miles away
Nearby Hospitals
- Vicarage Field Health Centre 3.27 miles away
- Barking Hospital 2.96 miles away
- Oxlow Lane Clinic 2.91 miles away
- Henley Road Surgery 2.39 miles away
- H A S S Centre 1.53 miles away


text:
It is difficult to write reviews of camps like this because it all depends on expectations. People expecting a Four Seasons will be bitterly disappointed - this is a camp that is seasonal with long supply routes, it can get muddy, there is only solar power for the tent lamps and they are dim, there is no wifi or the other things people paying a lot of money expect. But that is not the point - I stayed here for 2 nights. One day at lunch the Serengeti migration passed right through the camp, with thousands of wildebeest walking and running right by us. While we were having dinner, there were lions right near the toilet tent and that same night there were four lions who fell asleep where we did the campfire. 8 more lions were in the bush near my tent the previous morning. Being close to the action allows unique experiences. In one day I filmed lions mating six different times. I saw a number of the aftermath of kills -- lions and cheetahs eating. And there was a unique view of 12 wild dogs too. The staff here work extremely hard, there is a good camp atmosphere around the communal dinner table and the food is great. This camp delivers unique experiences. The days I spent around Ndutu are ones I will remember for decades.
- Official Description (provided by the hotel):
- Perched on the edge of a permanent marsh,LEMALA NDUTU is the ultimate amphitheater for the wildebeest migration between December and March. 12 Suite tents are relocated from the Northern Serengeti to this stunning Ndutu site to capture the boundless drama that accompanies the migration. The migration arrives in December and begin calving in February. The camp is situated inside the NCA, near the border of Southern Serengeti, in what is perhaps the finest location in the whole of Ndutu. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com so you can book your Lemala Ndutu Tented Camp reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.


text:
Apartment Features
- Air Conditioning
- Alarm
- Cable Ready
- Ceiling Fan(s)
- Dishwasher
- Fireplace
- Hardwood Flooring
- Internet Included
- Microwave
- New/Renovated Interior
- Oversized Closets
- Some Paid Utilities
- Stainless Steel Appliances
- Vaulted Ceilings
- View
- Washer & Dryer In Unit
- Garbage Disposal
- Patio
- Refrigerator
Special Features
- Disability Access
- Furnished Available
- Pets
- Short Term Available
- Corporate Billing Available
- University Shuttle Service
Community Features
- Accepts Credit Card Payments
- Accepts Electronic Payments
- Basketball Court(s)
- Business Center
- Campus Shuttle
- Clubhouse
- Emergency Maintenance
- Extra Storage
- Fitness Center
- High Speed Internet Access
- Hot Tub
- Pet Park
- Public Transportation
- Swimming Pool
- Tennis Court(s)
- Trail, Bike, Hike, Jog
- Wireless Internet Access
- Conference Room
- Media Center
- On Site Maintenance
- On Site Management
- Racquetball Court(s)
- Recreation Room
- Pet Friendly
Additional Features
- Signature Suite Available
- Corporate Units Available
- 3 Swimming Pools & Hot Tubs
- Sand and Water Volleyball
- 24 hour Sauna
- Car Wash Station
- Outdoor Racquetball Court
- Two lighted basketball courts
- Billiards Lounge
- Picnic Areas stationed throughout property
- Utility Packages Available
- Two lighted tennis courts
- 24 hour study lounge & business center
- 24 hour computer lab
- 2 Club Houses
- Pet Park
- Weight restriction: 80Deposit waived for adopted pets Pets Accepted
- Cats Allowed
- Dogs Allowed
- Deposits start at $250 and goes up to $350 and Pet Rent starts at $25 and goes up to $35, this varies on size and age of pet. Maximum of 2 pets allowed per apartment. Call for Policy
Floor Plans & Pricing
Prices and availability subject to change without notice.
Property Details
The Polos of Gainesville
Only minutes from UF, Shands and Downtown, The Polos is Gainesville's finest resort community. Bask in the Florida sun at one of our three sparkling pool decks or get your game on at our 24 hour fitness center. The Polos offers a variety of sports facilities such as sand volleyball, racket ball and tennis. Students will enjoy our quiet fireside study lounge and computer lab complete with complimentary printing. Every home boasts a spacious floor plan, a private screened patio and a washer and dryer. Customize your living space by choosing a home that features a vaulted ceiling or fireplace. For your convenience, The Polos can simplify your life by providing cable, furniture and utility packages. Enjoy your new home where we specialize in Less Work & More Play! Internet is now included in every apartment.
Lease Terms
Variable, 12-Month
Office Hours
Open Today
Ratings & Reviews
28 Reviews for The Polos of Gainesville
Sort by:
The Polos are in a good location, the grounds are attractive and well-kept. The staff is very friendly and helpful, and the amenities are nice as well.
Apartments are quiet, spacious and affordable. The grounds are lovely and well maintained. Staff- both in the office and those working maintenance- are friendly and competent.
Good price
Staff is friendly and knowledgeable. I am always supplied by what I need. Quiet living environment, very accessible to the rest of Gainesville, I enjoy very much living at the Polos.
Maintenance is great - very quick to repair things that break, and willing to replace things that can't be repaired
What is a Certified Resident?
A resident who lives at the property and has completed a resident satisfaction survey.
Advertising properties provide a list of email addresses of its residents to Kingsley Associates, a third party market research firm, who then sends out the satisfaction survey to the residents. Properties may also conduct satisfaction surveys of its residents through other third party market research firms. In those cases, we will publish those survey responses as long as the questions conform to our standard survey format.
Because reviews are from Certified Residents, and are managed by a third party, you can feel confident that the feedback provided is accurate and trustworthy.
General Directions
Please call for directions.
Helpful Information
Fax: 3523350479
Visit the property's management website
We consciously attempt to create a positive, principle-oriented company culture. Job offers to all prospective Team Members come with a copy of the highly acclaimed best-seller, The Seven Habits of Highly Effective People, by Stephen R. Covey.Prospective Team Members are assigned to look it over and make sure they are comfortable with its principles.


text:
If you are interested in link exchange with us, please place a reciprocal link on your site and email our internet marketing company at firstname.lastname@example.com
High Touch Recommends The Following Services:
Connecticut Movers - Noahs Ark Inc provides moving services in connecticut. Noahs Ark is a Connecticut licensed moving company with more than 30 years of experience and A+ rating on BBB.
New Jersey Movers - US Express Moving is a reliable local moving company located in New Jersey. They provide all moving services including storage, art moving, piano moving and commercial moving to all New Jersey residents and businesses.
Chicago Movers - Geniemove Chicago movers for piano, furniture, residential and commercial moving services to All-American clients especially in Chicago surrounding areas.
Mini Storage NY - NYC Mini Storage offers complete storage services across New York and its boroughs including Bronx. Throughout years of storage activity, NYC Mini Storage has worked inflexibly to gain their reputation for quality and professionalism.
Northern New Jersey Storage Warehouse - Suburban Warehouse offers an affordable storage solution in their warehouses in Randolph, New Jersey.
Moving Supply - Ubox.com provides a Manufacturer Direct option to buy discount moving boxes, moving supplies, and moving kits.
Brooklyn Houses - Massada Home Sales has also been in the business for more than 30 years. This real-estate company specializes in selling houses in Brooklyn. Check their website for their large Brooklyn houses for sale inventory.
Locksmith NYC - SOS Locksmith may be one of the first locksmiths in the Manhattan. They are known for their professionalism and honesty. SOS Locksmith provides all locksmith services - we truly recommend SOS locksmith!
Land Watch GovernmentAuction.com provides government land for sale at auction. This government auction site provides listings from all over the United States. Invest in government land for sale at a great value.
Web Design NYC - ORAIKO CORP provides 3 main services: web development, SEO & Internet marketing services, software development. If you read this then obviously these guys are doing something right. Yes, they have developed this site and they also market it. We truly recommend this professional company.


text:
Top 10 Fonts Web Designers Love [Infographic]
180 Power Words Guaranteed to Get More People Clicking Your Links #SocialMediaMarketing #ContentMarketing #Infographic
Why ecommerce retailers should never place products on the homepage | Econsultancy
8 Ways to Improve the Usability of Your #Website #Marketing #Business
Build websites or looking to have one built? Remember these 51 website design tips that will help to improve the overall design and user experience.
18 Rules for Using Text - If your document has text in it, follow the rules. You'll start to see a world of difference in your professional documents, whether they be formal reports, brochures, or even your personal résumé. These rules for using text apply across the board.


text:
Worship and Christian Education
2608 Browns Lane, Louisville, KY 40220
Telephone: (502) 452-1777, Monday through Friday, 9:00 AM to 1:00 PM
Sunday School for all ages at 9:15 AM; Sanctuary Worship at 10:30 AM
We initally meet each Sunday at 9:00 AM for a light breakfast and announcements. Then, Sunday School classes for all ages begins at 9:15 AM, followed by Sanctuary Worship at 10:30 AM.
For more on our Sunday Schedule, check out Our Sunday Morning Experience and Christian Education for All Ages.
June 28, 2017
Saint Andrew UCC celebrates its
sixth year as an
Open and Affirming (ONA) congregation!
We welcome everybody (as Jesus would!); and "discerning the signs of our times," we are particularly intentional about extending a special welcome to, and full affirmation of, folks from among the Lesbian, Gay, Bisexual and Transgender (LGBT) community. For more information, see our ONA page.
Join Us!
If you are curious about what a truly nurturing community of believers is like, then you should go to our Join Us! page.
What is the United Church of Christ?
Find out about the United Church of Christ (UCC) and the history, posture and beliefs of our denomination on our About the UCC page.


text:
Our second year of running courses for AMIDEAST. These courses, which take place in the dunes close to Muscat, involve young people from Oman and the USA sharing a challenging journey and a series of problem solving activities designed to promote greater cultural understanding.
Mission: To help people discover and develop their potential to care for themselves, others and the world around them through challenging experiences in unfamiliar settings.


text:
Advance Your Career
Completing a Rady School certificate program represents a significant academic accomplishment and demonstrates a higher level of competency. While less time and money are required for a certificate when compared to a degree, certificates are often seen as more practical, focused and relevant to today's workforce. Certificates provide an accelerated way for working professionals to obtain that extra edge for a promotion or to help one forge a new career path.
All certificates are issued from the Rady Center for Executive Development and do not count towards any outside degree or certificate. For example, our classes do not count towards PMP or Microsoft certifications.
Mindful Executive Certificate
Communication Certificate
Leadership Certificate
Marketing & Strategy Certificate
Finance Certificate
Excel Certificate
- Excel Essentials
- Excel: Pivot Tables, Macros, VBA & more
- Excel: Dashboards, Power Pivot, Power Query & more
- Excel IV*
- *Excel IV will be offered around June 2017. Date TBA.
Questions? Visit our FAQ page.


text: Marketters Job opening: healthcare jobs, jobs in Yaba, Lagos, Nigeria Share on Marketters Suggested salary: NGN 25,000 Location: Yaba, Lagos Last update: 2015-11-16 Ad description marketters and distributors wanted 4 marketing.For more job details, please contact me ATTENTION! Do not make any advance payments to any seller on Mobofree, not even for delivery! You may get scammed by unfair seller. Please report abuse to help us stop scam! To keep yourself safe, make sure to follow our Safety tips!


text:
So I'm sitting here building a bunch of virtual machines using PowerCLI. I decided to start with a spreadsheet into which I've collected many of the important things about a virtual machine:
Since I don't actually build VMs every day, and I haven't focused on a build process (like I should, I know!) yet, this spreadsheet was a first draft of a build process and it was made for humans, not machines. What does this mean? Well, obviously, by looking at the Memory column, any of you would guess that the unit of measurement is gigabytes. However, VMware happens to measure memory in megabytes.
Long story short, I wrote a quick one-liner in PowerShell to "spec out" the newly-cloned virtual machines using this spreadsheet. As I said, it's not a build process yet, but it will be when I'm done. Baby steps. The one-liner looks like this ($t is the variable that holds the data obtained from the spreadsheet, using a simple Import-Csv cmdlet):
1: $t | % { Set-VM -VM $_.name -NumCpu $_.cpu -MemoryMB $_.Memory }
Once I started that running, I quickly realized that 8MB VMs would do me no good. So, I amended my script to this:
1: $t | % { Set-VM -VM $_.name -NumCpu $_.cpu -MemoryMB ( $_.Memory * 1024 ) }
That's when I got a really weird error:
Set-VM : Cannot bind parameter 'MemoryMB'. Cannot convert value "888888888888888888888888888888888888888888888888888888
888888888888888888" to type "System.Int64". Error: "Value was either too large or too small for an Int64."
Yuck! That one threw me for a loop for a moment until I realized the important lesson of the day. When you use a technique like Import-Csv, the resulting object is a bunch of strings! What happens when you multiply a string by a number in PowerShell? Yes, it's effectively a concatenation. So in my case, the number 8 followed by one-thousand-and-twenty-three of the same. Nice, when that's what you intended. That was not my intent this time!
So to round this post out with a fix, here's the right way to get the intended result, which was to turn "8" into "8192":
1: $t | % { Set-VM -VM $_.name -NumCpu $_.cpu -MemoryMB ( [int]$_.Memory * 1024 ) }
Note the "[int]" there before the $_. That will convert the resulting property to an integer BEFORE performing the multiplication. That's the key here, it has to happen before (in the order of precedence), otherwise I end up with a bunch of 8's. Now, I happen to know the order of operator precedence in PowerShell well enough to know that the above would work without any doubt, but if you aren't sure about a particular piece of code, you can always surround a portion of a statement with parentheses in order to ensure that you get the order that you need.


text:
Leeds 10K
9th July 2017 Join the Day One team and take part in the 11th Leeds 10K! It's a flat, fast and fun course and entries are open now! For your registration fee of £23, you will receive chip timing, a finishers goody bag, quality tech t-shirt, exclusive medal and fantastic memories. Contact firstname.lastname@example.com for a Read more »


text:
Feldspar Raymond Pulverizer for Sale,Feldspar Raymond Pulverizer Suppliers
Hongcheng has more than 30 patents, has the right of export, and is certificated by ISO9001:2008 international quality management system is a national high-tech enterprise.
Feldspars are a group of rock-forming tectosilicate minerals that make up as much as 60% of the Earth's crust.
Feldspars crystallize from magma as veins in both intrusive and extrusive igneous rocks and are also present in many types of metamorphic rock.Rock formed almost entirely of calcic plagioclase feldspar (see below) is known as anorthosite.Feldspars are also found in many types of sedimentary rocks.
Uses of Feldspars
Feldspar is a common raw material used in glassmaking, ceramics, and to some extent as a filler and extender in paint, plastics, and rubber. In glassmaking, alumina from feldspar improves product hardness, durability, and resistance to chemical corrosion. In ceramics, the alkalis in feldspar (calcium oxide, potassium oxide, and sodium oxide) act as a flux, lowering the melting temperature of a mixture. Fluxes melt at an early stage in the firing process, forming a glassy matrix that bonds the other components of the system together. In the US, about 66% of feldspar is consumed in glassmaking, including glass containers and glass fiber. Ceramics (including electrical insulators, sanitaryware, pottery, tableware, and tile) and other uses, such as fillers, accounted for the remainder.
Feldspar applications include:
Paints and Coatings
Plastics and Elastomers
Engineered Stone
Abrasives and Polishes
Feldspar Raymond Pulverizer can be used to grind barite, calcite, kalium, marble, glass, feldspar,dolomite, kaolin, bentonite, talcum, talc, diatomite, gypsum, etc.Feldspar Raymond Pulverizer (Raymond mill) is widely used in the metallurgy, building, chemical industry, and mining field..
If you are interested in our Feldspar Raymond Pulverizer, please refer to:
Tel: +86-371-55912969
Email: [email protected]
ADD:Wulong Industrial Park, Zhengzhou City, Henan Province, China
Website: http://atletiek.co.za/


text:
Retro Patio Chairs
Sunday, February 26th, 2017
In the event that you need tips on how to redesign or redecorate your home, this magnificent Patio Boat snapshot collection might your personal method. Most illustrations or photos at Patio Boat snapshot stock will assist you to get the pattern you must have through the design suggested. And you could undertake this producing home while using the matching check and additionally setting to help Patio Boat photograph stock. This approach excellent Patio Boat photo stock offers several advantages back to you, 1 you can get yourself is mostly a eternal design. By means of the plan out of Patio Boat photograph collection, your household has to be extremely beautiful along with where you invite place to reside. Take always into account to find the concept of Patio Boat snapshot stock as per your preferences so that the house may be the preferred set so you might spending some time. A residence inspired as a result of Patio Boat photo collection could also be an excellent location to hang out with mates and also household.
Patio Boat picture gallery shows your blend from wonder and coziness that can spoil someone that is in it. You can actually fill out an application your points created by fantastic Patio Boat snapshot stock to your property to generate a dwelling which has a excellent conditions. And yourself will please let your private creativity describe as a result of mixing a lot of styles coming from Patio Boat image stock. And it is also possible to merge that types with Patio Boat image collection with your personal type to produce a check that is definitely really custom. I highly recommend you love this particular Patio Boat graphic gallery of which contains HD shots. The grade of this photos offered simply by Patio Boat graphic stock will allow you to start to see the pattern by having a clear along with highly detailed, thus many illustrations or photos tend to be worthy to help you transfer.
Sunday, February 26th, 2017
Thursday, February 23rd, 2017
Saturday, February 25th, 2017
Monday, February 20th, 2017
Tuesday, March 7th, 2017
Sunday, February 19th, 2017
Saturday, March 4th, 2017
Tuesday, February 21st, 2017
Monday, February 20th, 2017
Saturday, February 18th, 2017
Monday, March 6th, 2017
Thursday, February 23rd, 2017