text:
we have been volunteers and GALT adopters for year, well over a decade. We are continuously grateful for GALT's dedication to caring for all the greyhounds in its custody. No expense is spared in medical care. At one point two different fosters were on two different special foods. Now one if taking extra meds. A relatively recent addition is Foster checkpoints where all fosters are bathed, have nail trims, teeth and ears and weight checked. All dogs are closely monitored and cared for and volunteers are appreciated.
Previous Stories
How I adore an organization where every bit of the funds goes to the purpose for which it is intended, to help dogs and not to some highly paid executive. we have volunteered with GALT for years and years now. It is a rewarding venture made all the more so as we share our experience with so many other caring volunteers. This is the most worthy of support animal adoption group. There are not enough superlatives.
GALT is the best greyhound adoption group anywhere. Their dedication to the hounds and their adopters & volunteers is just astounding! They are very helpful to their adopters and have built a wonderful network of supporting medical facilities that help us all to keep the hounds happy & healthy. And the events are just a blast! If you are looking for a wonderful 4-legged companion, check out GALT!
Previous Stories
It is my honor and privilege to continue my support of GALT. I have been a proud member and supporter for many years. GALT lives it's "no grey turned away" vision every single day. They don't turn away from a greyhound/sighthound in need. GALT's volunteers are the lifeblood of this organization. I am so very happy to be a part of this amazing organization .
GALT is the finest greyhound adoption group I've ever been involved with. They take in any hound in need and treat each and every one of them with the highest level of care and caring. I can't say enough about how professionally this group is run. The leadership is superb and the volunteers are all wonderful.
GALT is the gold standard among greyhound adoption groups, they go above and beyond for the hounds, dedicated and passionate, we've adopted 3 hounds so far, more in the future!, I can only say positive things about this organization
I love everything GALT does to help the hounds find forever homes and the positive experience I have had as a volunteer. We all pull together to get the job done professionally.
I have the privilege of having two GALT Greyhounds and they are the my loves. I am also an adoption coordinator and foster for GALT. Such a wonderful organization that does such great things for the greyhounds, from strays, to hounds off the track, to puppies that have been dumped, to taking greyhounds (and greyhound mixes) from hoarding situations. All the volunteers work together to get the hounds ready for their forever homes.
Previous Stories
GALT is an amazing rescue organization that has a fantastic group of volunteers. Each and every volunteer does a wonderful job of preparing the hounds for adoption and then following up with new adopters to help make the transition better and a more pleasurable experience for both the hound and the human.
As a GALT volunteer I so enjoy working with the other volunteers and helping with the hounds. it is a first class rescue organization and i am proud to say I am a part of it!
I have the privilege of living with three GALT greyhounds in my home. I cannot thank this fine organization enough for trusting me with them and for supporting me in my efforts to give them the best life possible. GALT volunteers are very professional and truly love their charges. They are very helpful and dedicated to the hounds. I am proud to be a GALT member and give my support to GALT.
Previous Stories
I am proud to be a GALT member for many years now. GALT volunteers are the very best and always rise to the occasion to help hounds in need. I like knowing that my donations are used to help the dogs exactly as expected. Thanks, GALT, for 15 years of dedication to "no grey turned away!"
GALT is still as amazing as when I first encountered it. The care they show their dogs, adopters, and volunteers is unprecedented.
Previous Stories
I can't say enough about how amazing GALT is. The support you get through adoption and as a volunteer is amazing. I'm proud to be a part of this organization.
I am so proud to be part of such a wonderful organization. I have fostered puppies (and other dogs) for GALT. First of all, taking in litters of puppies is an effort in itself, but moreover, these poor darlings would be dead or homeless if GALT didn't step in. Below is a photo of a puppy that GALT saved from terrible conditions. Thank you GALT for all you do!
Previous Stories
I encountered GALT from a friend's referral when we moved to the DFW area. I didn't look them up at first, but my friend kept nagging me and saying...this is the best greyhound rescue in the country. After awhile, I connected with GALT, and began fostering and volunteering occasionally, but the entire organization is so friendly and well-run that I'm now fundraising for them too. The best part about GALT is that they are an all-volunteer organization, so every single dime goes to the care and placement of the rescued dogs.
The Greyhound Adoption League of Texas lives its mission everyday. The greyhounds are taken care of from intake to adoption by this team of dedicated volunteers.
Previous Stories
The Greyhound Adoption League of Texas is an outstanding all volunteer organization whose mission remains true and strong since its inception. Incredible people helping these retired athletics find their forever homes. Absolutely the best adoption group ever!!!


text:
Plans To Merge BTP With Police Scotland Agreed
The Scottish Government says the move will make railway policing more accountable but critics include trade unions and BTP officers.
A fourth man has appeared in court on charges related to the purchase of Rangers FC in 2012.
Paul Clark attended Glasgow Sheriff Court a day after former Rangers owner Craig Whyte and ex-chief executive Charles Green were charged along with David Whitehouse.
Clark and Whitehouse both worked for the club's administrators Duff and Phelps.
Clark, from Surrey, is accused of an offence under Section 28 of the Criminal Justice Licensing Act 2010 which covers serious organised crime.
The 54-year-old, who also faces a charge of conspiracy, made no plea or declaration and was released on bail.
Whyte, 44, Green, 62 and Whitehouse, 50, were charged with the same offences when they appeared at court on Wednesday.
Green also faces a charge of fraud and an offence under Section 190 of the Companies Act 2006.
All three made no plea or declaration and were granted bail.
Whyte took control of Rangers in May 2011 but by the summer of 2012 Green had taken charge at the Ibrox club by purchasing Rangers' assets and business after it went into administration and liquidation earlier that year. He stepped down as chief executive in 2013.
The Scottish Government says the move will make railway policing more accountable but critics include trade unions and BTP officers.
A building at Edinburgh Napier is having cladding removed, but students aren't being evacuated from the halls of residence.
A high-end jewellery store has been robbed in a raid at the five-star Gleneagles Hotel.
A review into adverse events at a maternity unit where some babies died has called for improvements in training and better family engagement.
Ariana Grande One Last Time
Whitney Houston How Will I Know
Ellie Goulding Love Me Like You Do
Now playing: The best feel good music
Deposit £10 to get a £40 Welcome Bonus - That's £50 to play bingo, slots and more!*
Over 50 tracks to make you feel-good. New album out now...
Find your local four day weather report here.
Make Heart the soundtrack to your day and you could be a winner with great prizes up for grabs throughout the day.
Find out more about some of the companies advertising on Heart Scotland- East.

subset: commoncrawl


text:
Hand Dyed Material
Vibrant hand-dyed colours in a choice of Fine Wool, Woollen Blanket and Nylon Hose material. Hand dyed swatches help create wonderful effects and enhance your hooking and prodding. Choose fine, blanket or hose material in Red, Pink, Blue, Purple, Green, Turquoise, Orange, Gold, and Yellow.
Fine Wool
Top quality new wool, great for hooking and will give good results in Proddy
- Swatch 16"x14" £4.50
- Swatch 34''x14'' £9
- 1/2yd £18
- 1 yd £36
+ p&p £3 up to 2 yards
Woollen Blanket
Recycled and ideal for making thick colourful proddy mats. Also suitable for hooking if cut finer.
- 1 yard 36″x36′ £14.00 p&p £3 up to 3 pieces
- 1/2 yard £7
Woolly Bundles
Pack of 4 gorgeous hand dyed soft wool Swatches each one approx. 14″x16″, perfect for hooking, proddy or other textile projects.
Choose from:
- 4 pack assorted colours £18
- 4 pack = 4 pieces of one colour in different shade
+ p&p £3 up to 2 yards
Hand Dyed Wool Yarn
100% Hand dyed chunky yarn suitable for Punch Hooking and Hooking beautiful selection of fabulous colours 100 grms £5.95 (please contact for colour selection)
Nylon Hosiery
Dyed hose suitable for hooking fine to wide cut. £2.50 per pack (minimum order 3 packs, p&p £3)


text:
Hips and Hair
Hips and Hairs' 100% cotton, black Real World '92 Crop.
100% cotton
Made in the USA
Megan is 5' tall 25" waist wearing a small.
Subscribe to our newsletter and always be the first to hear about what is happening.
Grit N Glory is a Rock N Roll lifestyle Boutique featuring The Megan Massacre Tattoo Studio
186 Orchard st. New York NY 10002
(212) 253 2775
© 2017 Grit N Glory.
Ecommerce Software by Shopify


text:
This property has been removed by the agent. It may be let or temporarily removed from the market
Property Description
Letting information:
|Reduced on Rightmove:
|04 January 2017 (170 days ago)
Key features
- Charming studio flat
- Located in a period villa in Notting Hill
- Presented in excellent condition throughout
- Modern open-plan kitchen
- Well proportioned studio room
- Close to amenities and transport links
Full description
LONG LET. This charming studio flat offers fully refurbished living space in excellent condition and features a well proportioned studio room, modern open-plan kitchen and bathroom located in a period villa in Notting Hill.
Pembridge Villas is a popular address moments from the trendy shops and pavement cafés of Westbourne Grove and the bars and restaurants of Notting Hill Gate. For direct travel links into the City, Notting Hill Gate Underground Station is nearby.
More information from this agent
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/property-to-rent/property-56715733.html
Externally hosted floorplan
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/property-to-rent/property-56715733.html
Map & Street View
View in fullscreenView in fullscreen
Street View is unavailable in this location
Disclaimer - Administration fees may apply when renting a property in England, Wales or Northern Ireland. For more details, please contact the agent.
Property reference 1002932. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by Foxtons, Notting Hill.
Please refer to our Renting Guides for further information on how to safely rent a property.
Map data ©OpenStreetMap contributors.


text:
Starbucks Debuts Four Lady Gaga-Inspired Vegan Drinks
The Cups of Kindness collection feature coconut milk-based, refreshing summer drinks created to benefit Lady Gaga's anti-bullying organization Born This Way Foundation.
Today, international coffeehouse Starbucks introduced The Cups of Kindness collection, a line of four vegan drinks created to benefit the Born This Way Foundation—singer Lady Gaga's youth empowerment organization. The line-up consists of two new concoctions, Violet Drink (Very Berry Hibiscus Starbucks Refreshers blended with coconut milk and ice) and Matcha Lemonade (finely ground Teavana matcha green tea and lemonade shaken with ice), along with the existing Pink Drink (Strawberry Açaí Starbucks Refreshers mixed with coconut milk) and the Pink Ombré Drink (Cool Lime Starbucks Refreshers, Teavana Shaken Iced Passion Tango Tea, and coconut milk). Starbucks will donate a minimum of $250,000 (or $0.25 of each sale from the new collection) to Lady Gaga's foundation, Born This Way, that aims to help children empower themselves to fight bullying, and its new project Channel Kindness—an online platform encouraging young people to document their experiences of compassion in their communities. The vegan collection will be available at participating Starbucks stores across the US and Canada until June 19.
Punk Rock Drummer Rolls Out Vegan Food Truck
David Witte from thrash band Municipal Waste will be serving vegan cheese-slathered chimichangas streetside in Richmond, VA.
Read More »
Vegan Cinnamon Roll Shop to Open in Pennsylvania
Cruelty-free bakery Cinnaholic will bring its DIY cinnamon rolls to Lancaster county in July.
Read More »
Target Pulls Hampton Creek Off Shelves
The retail giant disabled registers to discourage shoppers from purchasing the company's vegan condiment Just Mayo, and 19 other products.
Read More »
Vegan Brand Named First Zero-Waste Facility of Its Kind
Earth Island—the production facility for iconic vegan brand Follow Your Heart—becomes the first plant-based manufacturer in the nation to receive Gold Level Zero-Waste certification.
Read More »
Impossible Burger Debuts at 11 Burger Bars in Texas
Burger chain Hopdoddy adds the popular plant-based patty to its menus in Austin, Dallas, Houston, and more.
Read More »
More Flashbacks
- Czech Republic to Ban Fur Farms
- 132-Year-Old Lobster Set Free by Long Island Clam Bar
- Smoked Meat Bar Now Sells Vegan Ribs
- Doctors Call for Plant-Based Meals in Hospitals
- Factory Farms Cause Lung Damage to Nearby Residents
- Reducing Cost of Vegetables Saves 150,500 Human Lives
- BREAKING: NYC Bans Use of Wild Animals in Circuses
- Vegan "Love Latte" Debuts for LGBTQ Pride Month
- Documentary About Vegan Music Legends Debuts in 2018
- Pennsylvania's Dairy Industry Continues to Crumble
- Couple Could Afford Greek Vacation After Going Vegan
- Middle Eastern Princess Invests in Vegan Cheese Startup
- Detroit Vegan Soul Opens in Fast-Food Dominated Area
- Pamela Anderson to Open Vegan Eatery in France
- Dairy Plant Drops 11 Milk Suppliers Due to Surplus
- Pea Protein-Based Vegan Greek Yogurt Coming Soon
- Kellogg's Invests in Vegan Smoothie Company
- Starving Pigs Saved from Small Island in California
- PDX Eateries Combine to Form Vegan Hotspot
- Teen Creates Animal Activist Mobile Game


text:
0 jobs
9 ms
Within a radius of km of
Filter by date ▼
Last 24 hours
Last 3 days
Last 7 days
Last 30 days
Filter by source ▼
Job boards
Placement agencies
Jobs » Iphone Devloper
Jobs 1 - 10 of 0
Iphone Devloper jobs
There are 15 Iphone Devloper-related jobs on Neuvoo India. Submit your application now and get the best job.
Job descriptions and categories including this search
Examples of corresponding vacancies are Industry Analyst, Advertising Copywriter, Cruise Staff, Financial Services Representative, Meat Clerk or Glass Grinder. These job vacancies are often linked to categories like PR, Education, IT, Management, Services or Communication.
Employment statistics
Regions with the largest number of vacancies related to 'Iphone Devloper' are Tamil Nadu. Cities with the highest number of hits are Chennai.
Top employers offering Iphone Devloper jobs
Corporations posting the most Iphone Devloper-related jobs are Lutech Resources India Pvt Ltd, Superseva Services Private Limited, optimus, Compass Systems Pvt. Ltd., Google or Dharra Advance Business Solutions.
Neuvoo India: the the largest job search website across India
Neuvoo India is the largest job search website in the country with over 1636222 job opportunities available, 15 of which are Iphone Devloper-related.Show more...
Never miss a job. Get new jobs emailed to you everyday.


text:
Donor News
Transformative $30 Million Gift from Samuelis to Fund Convergent Science Building
A $30 million gift from the Samueli Foundation will help fund a state-of-the-art convergent science building, expanding the University of California, Irvine's ability to conduct large-scale, collaborative and cross-disciplinary research in engineering, computing and physical sciences.
With $1 Million Gift, UCI Aims to Become First University to Launch Rocket into Space
University of California, Irvine students will "shoot for the moon" thanks to a $1 million gift from Base 11, a nonprofit STEM workforce development and entrepreneur accelerator.
Former Dean, Wife Honored at Presidential Chair Celebration
It was standing room only in the Calit2 auditorium last night as 125 friends, family members and supporters of former Samueli School Dean Nicolaos Alexopoulos joined together to honor him and his wife, Sue Curtis Alexopoulos, after a UC Presidential Chair was named for them.
The Henry Samueli School of Engineering at UCI receives $9.5 million gift
Stacey Nicholas' Opus Foundation has made a $9.5 million gift to The Henry Samueli School of Engineering at UC Irvine.
Building Towers
The people of Catalonia, Spain, have a centuries-old cultural tradition of building human towers, or castells (castles).


text:
Models Own Pink Punch
This has been my absolute favourite nail polish this year, i love how bright and summery it is.... even if the weather isn't great it still brightens my mood :) . I cant seem to take to off i just keep re applying this one every time i do my nails. Im even on to my back up pot!
Im really hoping Models Own do there 50% off same again this year so i can get another one. I don't know why but when i know a company does offers like 50% off i cant bring myself to pay full price so im very glad i got two of these in the last sale to last me.Same with elf... they always have offers on!
Ive been wanting to try the Models Own Beach party from there Hed Kandi range too so that's top of my list for my next order ;) .
Bourjois 1 second nail polish remover
First of all the name is a bit of a lie... it takes about 4-5 seconds.Although this is a great product! it does take polish of super fast as without making a mess and saves on having to get cotton pads out too.Ive had this for a few month now and it still works really good but i have too stand it upside down for a while before i use it so the remover is covering the top of the sponge inside. I would purchase again because its alot quicker to use when im just wanting to do my hands quickly. You do still have to buy the usual polish and cotton pads to do your toes thought because this just wouldn't work for that.


text:
Looking for Basic school project Freelancers or Jobs?
Need help with Basic school project? Hire a freelancer today! Do you specialise in Basic school project? Use your Basic school project skills and start making money online today! Freelancer is the largest marketplace for jobs in the world. There are currently 17,764 jobs waiting for you to start work on!I Want to Hire I Want to Work
Basic school project Jobs:
|Price (USD)
|Price (USD)
|Office center and services
|I have a house in the city center of a small town in Colombia (South America). I will refurbish that house so it will serve as a conglomerate of offices that will provide different types of services to community. Services such as legal advice, accounting advice, printing and copy machines, office and school ware, medical services, etc will be offered. A Coffee shop will work also there. It is imp...
|Graphic Design, Logo Design, Illustrator, Photoshop Design, , Design
|Jun 22, 2017
|Jun 22, 20175d 22h
|Small node / react application
|Javascript, CSS, HTML, node.js, React.js
|Jun 17, 2017
|Jun 17, 2017Ended
|Write a Report about a creative piece of writing
|Hi ! I've written a 12-page long artistic text and would need someone to write a 1000 word report about it explaining why I did it, what it is about, what I hoped to achieve, what were my inspirations, explaining the thinking behind it, ... I like writing with the heart and follow my feeling but this time I need to write a very academic report (boring) and I was wondering if someone out ...
|Report Writing, Creative Writing
|Jun 15, 2017
|Jun 15, 2017Ended
|Android and Java Web Developer required
|We are working on a School project with a Web interface as well as an Android interface. Currently we need to add some features in both the modules like Daily Time Table management, Attendance feeding, Report Card,School Calendar. The basic prototype will be provided. All these features require small development. I am ready to pay [url removed, login to view] for this work. Interested candidates c...
|Java, JSP, Android, HTML5, jQuery / Prototype
|Jun 12, 2017
|Jun 12, 2017Ended
|Write me a Business Plan : 4c & Kite
|Hi, we have a kite surfing school in Tunisia, Gammarth area (4c & kite ) , we are targeting the local market Tunisia - business client 6 and Tourist from Algeria and Libya and Europe; We need a verry lite businees plan more oriented on market strategy to help us to develop the business and engage online marketing. we need some basic financial modelization to check the value adde of th...
|Business Plans
|May 31, 2017
|May 31, 2017Ended
|Kids Program Identity Program
|We have a basic concept of a logo and some fonts we plan to use but we need someone to work to piece together a few things to make the brand uniform and come to life. Its not a big project but also it does not suit a standard corporate approach. Instead it needs someone who can use some creative elements more relevant to the pre-school market and sports. We do need some illustrator skills and ...
|Logo Design, Illustration, Corporate Identity
|May 26, 2017
|May 26, 2017Ended
|French to English Translators
|We are looking for long-term translators in the French<>English language pair. For this particular project, we have several school degrees, diplomas and transcripts that we would like to translate from French into English. Some basic formatting is required. All applicants must possess a strong command of both languages and translation expertise in the language pair. If interested, please inc...
|Translation, Proofreading, English (UK), French, English (US)
|May 23, 2017
|May 23, 2017Ended
|CAR WASH POS Hardware Interface Project
|We are searching for help developing a new product for the car wash industry. Using our car wash locations as testing facilities. Let me give you some background on the self service car wash business in the US and what products are available. When a customer comes to a self service wash location they interact with a small box that we call a "Meter Box". This is a stainless steel box...
|PHP, Engineering, Software Architecture, Electrical Engineering, MySQL
|May 20, 2017
|May 20, 2017Ended
|Hire a Data Entry Clerk
|I am looking for a freelancer to help me with my project. The skill required is Data Entry. I am happy to pay a fixed priced and my budget is $30 - $250 USD. I have not provided a detailed description and have not uploaded any files. I am looking for a freelancer to help me with my project. The skill required is Data Entry. I am happy to pay a fixed priced and my budget is $30 - $250 USD. I have n...
|Data Processing, Data Entry, Excel, Web Search
|May 13, 2017
|May 13, 2017Ended
|Build a Website
|*** We are looking for you to provide us with an initial cost estimate, given the info provided below. Selected applicants will be provided with the detailed SRS project document, so that a final and accurate cost can be discussed. Both independent freelancers and agencies/teams are welcome to apply. We are looking for someone who is available to continue an ongoing relationship after the projec...
|PHP, Javascript, Website Design, CSS, HTML
|May 12, 2017
|May 12, 2017Ended
|Research names of Seattle schools within 10 mile radius
|I'm looking for a complete list of preschools within a 10 mile radius of an address in Seattle, WA, USA. I'm looking for the name, street address, website, and distance from home address for each school. I have an example spreadsheet filled out showing exactly what I'm looking for, in what format (the info filled is fake, FYI). I also have instructions for how to find the sch...
|Web Search
|May 8, 2017
|May 8, 2017Ended
|I would like to hire a Google SketchUp Expert
|Use Sketchup 2017 not pro Cant look that great as it's a year 7 project. Need the it done in the next 2 [url removed, login to view] school project require a very basic floor plan of the interior space, room dimensions and indication of doors, windows and placement of furniture perspective view of interior space rendered Ceiling height [url removed, login to view] Master bedroom [url removed...
|Google SketchUp
|May 3, 2017
|May 3, 2017Ended
|Build a Website
|Need a basic website for a school project. Will give additional details when contacted. need it to be done within 4 hours, should be fairly easy for experienced people.
|PHP, Website Design, Graphic Design, PSD to HTML, HTML
|May 1, 2017
|May 1, 2017Ended
|Project for raheelakhurshid
|Hi raheelakhurshid, I noticed your profile which includes Matlab, and would like to offer you my school work project. The school has assigned me to do a basic GUI using Matlab, which I am struggling to code and make a progress out of it. I will be very happy if you are willing to lend a hand, and from your work I can learn a few things or two as well. Prices are negotiable and details will be sen...
|Statistics, Finance, Research Writing, , Statistical Analysis, SPSS Statistics
|Apr 27, 2017
|Apr 27, 2017Ended
|Create a C program that will display a food menu and take the users order. Everything is explained in the description
|I need you to develop some software for me. I would like this software to be developed for Windows using C code ONLY**** not C++ Budget is 15$ Need done in one day this is for a school project ! Here are all the guidelines MUST FIT THESE REQUIREMENTS I WILL POST AN EXAMPLE OF WHAT THE CODE SHOULD OUTPUT BELOW Background Dr. Archibald is opening up a hamburger restaurant. He has hired some...
|C Programming, Windows Desktop, Software Architecture, C++ Programming
|Apr 27, 2017
|Apr 27, 2017Ended
|Build a Website
|I had build project in visual basic platform .net asp. with database sql server 2008 three tier architecture. The project was cab booking. Another project made for school regarding management system where all details of staff members, newly admission, reports deployment done.
|ASP, Visual Basic, HTML, Database Administration
|Apr 14, 2017
|Apr 14, 2017Ended
|Need help with basic woocommerce tasks
|Hello, I am the project manager for [url removed, login to view] It is an ecommerce site in development for a medium-sized business that sells products on Amazon. We already have someone handling product uploading, but we need help with basic and easily trainable administrative tasks. The tasks include: Associating product images with "FJSIN" numbers, which are our internal product trac...
|Photoshop, Virtual Assistant, eCommerce, Product Descriptions, WooCommerce
|Apr 11, 2017
|Apr 11, 2017Ended
|Sell A school & college management software to schools
|We have a school management software. You will have to search contact information of schools online and sell the software to the schools. It's a continually developing project having all the basic functionality to run an educational institution having modules like Attendance, Examination, Fee Management, Transport Management , Library management etc. The software is hosted online. You can sh...
|Apr 5, 2017
|Apr 5, 2017Ended
|We need an existing website rebuilt PHP - Code Ignitor
|First I want you explain, what Pindex is. Pindex is actually philanthropic projects of two young co-founders, who try to help to all students in our Country - Slovakia, in the future in Europe and then in World :). Now we are in Slovakia and in some kind in Czech Republic. Project was created in 2012 - when we had a time, we made some progress. Now we decided, that we want to focus more and deeply...
|PHP, Website Design, Graphic Design
|Mar 26, 2017
|Mar 26, 2017Ended
|Need and android app for school
|I need an android app for school. Features:: Project-1(Very Basic Mobile App) [url removed, login to view], videos (Under Activities.) [url removed, login to view] schemes, syllabus and homework, Holidays (Under Notifications) This is simple mobile app. The school can post activities and notifications which will be available to all mobile app users. Project-2 (Advanced Mobile App) [url r...
|Mobile Phone, Android
|Mar 15, 2017
|Mar 15, 2017Ended
Showing 1 to 20 of 918 entries
Hire the Top Basic school project Freelancers:


text:
Homes for Sale - New Hampshire & Maine Real Estate Search
Search New Hampshire & Maine Real Estate
Stunning Contemporary Cape located in one of Exeter's finest sub divisions. This updated home has a beautiful chef's eat in kitchen w/fireplace leading to a gorgeous family room with floor to ceiling brick fireplace and built in's. First floor master suite with plenty of closet space, large bath with soak tub and shower, another first floor bedroom as well. Hardwood floors are beautiful, and a lot of tile/granite as well. 2 more bedrooms on 2nd level as well a fully equipped theater room (room to expand 2nd level even more if needed) This is a very unique home, updated, immaculately maintained and ready to move in--views of Brick Yard Pond from the back deck. Conveniently located just a minute out of downtown Exeter and 5 mins to the highway.
Request More Information
Lot Features
Nearby Schools
Additional Information
Calculate your mortgage payment
Get more information about this property
Copyright ©2017 The New England Real Estate Network, Inc. All rights reserved. This information is deemed reliable, but not guaranteed. The data relating to real estate displayed on this Site comes in part from the IDX Program of NEREN. The information being provided is for consumers' personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing. Data last updated 06/26/17 07:07 PM


text:
Love Letters
I recently saw a rerun episode of Oprah. She had a couple on the show who had spent the past 40 years writing love letters to each other—everyday! Their letters consisted of three parts:
Part 1: How I feel today. "Dear Alton," Patricia once wrote after a fight. "I still feel separated from you because of last night." One letter from Alton expressed his gratitude: "I feel honored you think I'm a great parent."
Part 2: "I love you because…" Their letters were full of specific, endearing reasons they love each other. "I love you for giving the children their baths." "I love you for fixin' my supper plate tonight. It was good."
Part 3: A question of the day. Alton says these questions range from, "How do I feel when you're sick?" to "How do I feel when you're short with the children?" to "How do I feel when you do something special for me?"
What a great way to keep the communication lines open! As I listened, I couldn't help but wonder how this activity might work for other couples. Anything that gets couples talking is a good thing, and maybe by writing instead of speaking, couples can open up without worrying about interruptions, immediate reactions, or being overheard.
Join me and give it a try, won't you?
Comments are closed.


text:
• Wolfthorn is the sort of sophisticated wolf who wears a suit that has a suave, sweet orange scent• Old Spice Men's Deodorant overpowers stink with good-smellingness • Answer the smell of the wild with Old Spice Wild Collection • So easy to use you might accidentally put it on and only later realize your man-nificence
Old Spice Men's Deodorant reduces underarm odor for 24 hours. Turn the base to raise the deodorant and wipe armpits for long-lasting freshness. Deep in every man, beneath the people clothes and remembering how to drive, beats the heart of a wild animal. Old Spice Wild Collection taps into that animal magnetism and unleashes the power of a beast in his element. Wolfthorn smells like the seductive and mysterious night.. Old Spice Men's Deodorant overpowers even the burliest stink with overwhelming good-smellingness. Win the battle of natural selection: out-compete your enemies, attract nearby females, and answer the smell of the wild.
Dipropylene Glycol, Water, Propylene Glycol, Sodium Stearate, Fragrance, PPG-3 Myristyl Ether, Tetrasodium EDTA, Blue 1
Additional information on fragrance components can be found by clicking here
For nocturnal creatures
This area allows consumers like you to express their own opinions and comments. Old Spice does not represent or warrant the accuracy of any statements or product claims made here, nor endorses any opinions expressed by the reviewer.
This stuff smells like love. If love had a smell, it would be Old Spice Wolfthorn.
Yes, I recommend this product!
Yes, I am female and I use this deodorant! It smells nothing like a traditional male deodorant! It has a fruity scent that works with men and women body chemistry!! I think of starbursts when I smell it, not only that but it last really long throughout the hottest or most active days! I would most definitely recommend this to anybody looking for a strong, long lasting deodorant!
No, I do not recommend this product.
Deodorant fail - dial for raising the deodorant up seems to be stripped. It will not raise, therefore I can not use the full deodorant, less than 5% is usable because of this defect on a brand new deodorant.
Smells better than a bag of delicious, fruity Skittles.
This by far surpasses all of your other fragrances for deodorant, body spray and body wash. I love it!
Wolfthorn is the best new scent yet!! I hope they don't discontinue this anytime soon. I've tried everyone of deodorants and I think I have found the one I'll be wearing for a long time!
Who smells like that?, Whats that smell? I KNOW ITS ME. Is this cool or what?, People are asking me this most of the time. I've got some aroma freakiness with this smell I just love it. The scent is so unique for a deodorant. You know someone is going to ask you the same as soon as you smell it. I am going to stick with this scent as long as it exists. THANKS!!!
One of the best deodorants I have ever bought from any company. Highly recommended the smell is so unique, I guarantee you have never smelled this amazing scent ever.
I love the smell. What I don't understand, however, is how did they come up with the names Nocturnal and Wolfthorn? The smell is more fruity-like. So what does a fruity-smell deodorant has to do with nucturnalness? Or wolfness? Maybe it should've been called something that resembles a fruit.
I bought a stick of Wolfthorn and found the scent to be delicious. Very fruity. I noticed that the scent didn't last long and nor did the antiperspirant aspect. I then tried the same flavor in a body wash and although it has a pleasant smell, it is nothing at all like the deodorant's. I won't be buying this product again but I do still use your other stick deodorants and body washes.


text:
Habitat Evaluation Procedures (HEP)
The HEP web pages and repository contain most of the documents related to this survey effort. The repository is designed to be a searchable database and will be maintained for historic tables.
Coordinated Assessments
An effort to develop data-sharing among the fish and wildlife agencies, tribes, and regulatory/ funding agencies of the Columbia River Basin for anadromous fish related data.
Downloadable Data
To perform more sophisticated queries download the entire database in a Microsoft Access 2000 file that contains most of the tables that make up StreamNet data.
Maps and GIS Data
StreamNet's Interactive Mapping tools allow you to place fisheries related data in context as you query and display fisheries information for the Pacific Northwest.
Data Store
The Data Store is an archive of stand-alone data sets that are not integrated into the main StreamNet data system and contains a variety of data types.


text:
We stayed in one of the suites on the 8th floor of the hotel. We were able to get a weekend rate that was less than half the rack rate. The suite was a two level suite with the bedroom and main bathroom on the...More
Getting you more information on this room
Getting you more information on this room
"Rooms on the back of the property are preferable as they have river valley views."
"We chose the river valley view and enjoyed seeing a variety of power/paddle boats passing by."


text:
They got away from the typical stuff they had done in the past. They used even more zone blocking instead of running the counter trey a lot. They ran it outside on pitches and tosses, where he starts in a direction and then makes his cut using the vision he has.
I don't understand why Chicago didn't say, 'If you're gonna beat us deep, then beat us deep. But you're not going to run.' To me the Redskins spread them out because they knew the Bears would do like everyone else and put eight or nine in the box. But the Bears didn't do that. I guess they felt their front four could handle the Redskins' front five. That didn't happen. They couldn't handle our guys. They won it because they get a guy out of the box. From a linebacker's perspective when you run tosses and pitches, you get a guy in space. For a linebacker, that makes a play more difficult on a back like Clinton unless you're a sure-tackle guy.
The Redskins got a lot of matchups on a safety where they were one-on-one in a hole. Clinton was able to juke and miss and get extra yardage. He didn't get tackled by defensive tackles or linebackers. He was getting hit by safeties, so he's getting positive yardage and running over guys.
Using that play was great coaching. When you go against an overpursuing, aggressive defense like Chicago -- everyone knows that Lovie teaches them that you only have to play one gap -- then the backside guy has to make that play. The safety or the outside linebacker and those backs were reading it. You had guys pursuing with the toss and the pitch. The linemen would block to their right and hold their block, waiting for an open lane. The linebackers are thinking he's going to the front side, but that back is thinking cutback. As soon as he makes the cutback, he's one-on-one with a safety and you want your million-dollar running back to beat one person. When he does, he gets 12, 14, 18, 20 yards.
The backside linebacker has to be disciplined. The Bears are one of the youngest teams in the NFL, especially at linebacker. They have a great one in Urlacher, but even he's young and sometimes guys don't know all the nuances and adjustments and understand that, 'Look, they've run that play on you 14 times, when are you going to get the backside linebacker to make that play?' In 1998, we were playing the Steelers and they ran that same play with Bettis. They'd run a toss and he'd look for a cutback. They beat me two or three times and my coach said, 'Maintain backside support!' If the outside backer stays home and protects backside, that play is dead.
But they overpursued every time and Clinton smashed them. The line also did a great job getting on their blocks and staying on them.
One subtle defensive play was the difference in the game and it was made by Fred Smoot, one of my unsung heroes of the game. It was third and 11 in the fourth quarter around the six-minute mark. Fred had torn up his shoulder a couple plays earlier against Thomas Jones. Then he comes back in and they throw a quick receiver screen, putting him in a one-on-one position against Fred. The Redskins were in zone coverage and Fred was reading the quarterback as he backpedaled. He's reading his eyes and the Bears also had a receiver running upfield trying to pull Fred out. But Fred didn't bite. He was probably supposed to go with the other receiver, but he read the quarterback. The Bears had been driving and if they scored a touchdown on that drive it's a different game. But Fred made the play.
Editor's note: Eddie Mason is the co-founder of M.A.S.E. Training, along with James Thrash. They seek to train athletes spiritually and physically. For more information, go to masetraining.com.
X's & O's with Eddie Mason
Breaking Burgundy Top Stories
Morning Manny: Quarterback Position ReviewManny Benton reviews the Redskins Quarterback position prior to training camp.
Breaking BurgundyYesterday at 5:00 AM
Could Nate Sudfeld Be The 2018 Redskins QB?One man in the front office had high praise for the sophomore passer.
Breaking BurgundyTuesday at 5:00 AM
When Swearinger Sized Up Redskins Safety WoesThe new starting free safety had his eyes on the Redskins way before he entered free agency.
Breaking Burgundy06/08/2017
It's Rob Kelley's Job To Lose At RBContrary to popular belief, Redskins RB Robert Kelley has a strong grip as the team's lead back.
Breaking Burgundy05/31/2017


text:
To determine whether aerobic exercise training + weight loss (AEX + WL) would affect the expression of myostatin and its relationship with insulin sensitivity in a longitudinal, clinical intervention study.
Clinical Trials #: NCT00882141
Disclosure: The authors declared no conflict of interest.
Funding agencies: This research was supported by the grant (RO1-AG19310, K01 AG021457, P30 AG028747, P30 DK072488, P60 DK079637, and M01 RR016500) from NIH, Baltimore VA Medical Research Service, VA Research Career Scientist Award (ASR), VA Merit Award, VA Career Development Award (JB), Department of Veterans Affairs and Veterans Affairs Medical Center Baltimore Geriatric Research, Education and Clinical Center (GRECC).
Correspondence: A.S. Ryan (email@example.com)
To determine whether aerobic exercise training + weight loss (AEX + WL) would affect the expression of myostatin and its relationship with insulin sensitivity in a longitudinal, clinical intervention study.
Thirty-three obese sedentary postmenopausal women and men (n = 17 and 16, age: 61 ± 1 years, body mass index: 31 ± 1 kg/m2, VO2max: 21.9 ± 1.0 mL/kg/min, X ± Standard error of the mean (SEM)) completed 6 months of 3 days/week AEX + WL. During an 80 mU m−2 min−1 hyperinsulinemic-euglycemic clamp, we measured glucose utilization (M), myostatin, myogenin, and MyoD gene expression by real-time RT-PCR in vastus lateralis muscle at baseline and 2 h.
Body weight (−8%) and fat mass (−17%) decreased after AEX + WL (P < 0.001). Fat-free mass (FFM) and mid-thigh muscle area by computed tomography did not change but muscle attenuation increased (P < 0.05). VO2max increased 14% (P < 0.001). AEX + WL increased M by 18% (P < 0.01). Myostatin gene expression decreased 19% after AEX + WL (P < 0.05). Basal mRNA myostatin levels were negatively associated with M before the intervention (r = −0.43, P < 0.05). Insulin infusion increased myoD and myogenin expression before and after AEX + WL (both P < 0.001) but basal levels did not change. The insulin effect on myostatin expression was associated with the change in M after AEX + WL (r = 0.56, P < 0.005).
Exercise and weight loss results in a downregulation of myostatin mRNA and an improvement in insulin sensitivity in obese older men and women.
Myostatin is a member of the transforming growth factor beta family of secreted growth factors and is a significant negative regulator of skeletal muscle development and size ([1]). Transcription factors that regulate muscle differentiation include myogenic differentiation factor (MyoD), which is responsible for stimulating myoblasts to enter differentiation and join the muscle lineage, and myogenin which mediates terminal differentiation of myoblasts ([2]). Knock-out in the myostatin (Mstn) gene has led to dramatic increases in muscle mass in animals ([3, 4]) and mutations have been documented in a child ([5]). In humans, resistive training either acute or chronic reduces basal mRNA myostatin ([6]). One study showed a decrease in myostatin protein levels in middle-aged men after aerobic training ([13]), whereas two studies report significant reductions in myostatin mRNA levels after severe weight loss by gastric bypass surgery ([14, 15]). Thus, the effects of aerobic training and modest weight loss on these genes in skeletal muscle remain largely unaddressed, especially in older adults.
Animal model studies have demonstrated the importance of myostatin in glucose metabolism ([16]). Agouti lethal yellow and obese (Lepob/ob) myostatin knock-out mice have a reduction in body fat and improved glucose tolerance ([16]). Mstn−/− mice have increased glucose uptake measured by the hyperinsulinemic-euglycemic clamp ([17]) and when fed a high-fat diet, myostatin deficient mice also have improved muscle insulin sensitivity ([17, 18]). Myostatin knock-out mice have increased Adenosine monophosphate (AMP)-activated protein kinase, Akt, and phosphorylated Akt ([19]) which may explain the increased insulin sensitivity. Because in vivo insulin increases serine phosphorylation of Akt in healthy controls and men with impaired glucose tolerance ([20]), it is of interest to study the effect of insulin stimulation on myostatin in vivo. Based on the studies in the myostatin knock-out model and the limited evidence that exercise and weight loss can affect myostatin levels in humans, it was our interest to test the hypothesis that chronic aerobic exercise training + weight loss (AEX + WL) would reduce basal skeletal muscle myostatin protein and gene expression and be associated with improvements in insulin sensitivity. Therefore, the aim of this investigation was to examine glucose utilization and the basal and insulin-stimulated gene expression levels of myostatin, MyoD, and myogenin before and after a 6-month aerobic training + weight loss program in older, obese adults.
Subjects were healthy, overweight, and obese (body mass index, BMI > 25 kg/m2; range of 25-46 kg/m2) aged 54-77 years. They were weight stable (<2.0 kg weight change in past year), sedentary (<20 min of aerobic exercise 2×/week) and screened by medical history questionnaire, physical examination, and fasting blood profile. Individuals with untreated hypertension (blood pressure higher than 160/90 mmHg) or hyperlipidemia (triglycerides ≥ 4.5 mM, cholesterol > 6.2 mM, low density lipoprotein (LDL) cholesterol > 4.3 mM) were referred to their doctor for therapy and allowed to enter if they were treated with an antihypertensive or lipid-lowering drug that did not affect glucose metabolism. All subjects were nonsmokers, showed no evidence of cancer, liver, renal or hematological disease, or other medical disorders. The women in the study were postmenopausal and had not menstruated for at least 1 year. In addition, all subjects underwent a screening Bruce graded treadmill test to exclude those with asymptomatic coronary artery disease.
Thirty-three men (n = 16) and women (n = 17) met all study criteria and enrolled into the AEX + WL intervention. The Institutional Review Board of the University of Maryland approved all methods and procedures. Each participant provided written informed consent to participate in the study.
Subjects received instruction in maintaining a weight-stable, Therapeutic Lifestyle Changes (TLC) diet ([21]), by a registered dietitian (RD) 1 day/week for 6-8 weeks, before baseline testing. Subjects were weight stable on the TLC diet before baseline testing and were instructed to maintain the TLC diet throughout the study.
Subjects exercised at the Baltimore VA Medical Center GRECC exercise facility three times a week for 6 months using treadmills and elliptical trainers. Each exercise session included a 5-10 min stretching and warm-up phase and a 5-10 min cool-down phase. For the first 3-4 weeks, subjects exercised on the treadmill at ∼50-60% heart rate reserve for 20-30 continuous minutes as tolerated. Initially, time was progressed to 50 min. The duration remained for 50 min throughout the remainder of the intervention. Then, intensity was progressed to >60% VO2max and up to 80% VO2max. This progression of intensity typically took 4-6 weeks based on each subject's exercise tolerance. Exercise intensity, prescribed as a target heart rate range, was monitored using chest-strap heart rate monitors (Polar Electro, Lake Success, NY). There were no rest periods during the exercise sessions. All sessions were supervised by exercise physiologists.
All subjects also attended weekly weight loss classes led by a RD for instruction in the TCL diet. Compliance was monitored by 7-day food records (or 24 h recalls) using the American Diabetes Association exchange list system. Individuals were instructed to restrict their caloric intake by 500 kcal/day. The average compliance to the exercise sessions and weight loss classes was greater than 75%.
VO2max was measured using a continuous treadmill test protocol ([22]). Height (cm) and weight (kg), waist and hip circumference, were measured to calculate BMI and waist-to-hip ratio. Fat mass, lean tissue mass, and bone mineral content (BMC) (fat-free mass = lean + BMC) were determined by dual-energy X-ray absorptiometry (DXA) (Prodigy, LUNAR Radiation Corp., Madison, WI). A single computed tomography (CT) scan at L4-L5 region using a Siemens Somatom Sensation 64 Scanner (Fairfield, CT) was used to determine visceral adipose tissue and subcutaneous adipose tissue areas and analyzed using MIPAV (NIH Image Analysis Program). A second scan at the mid-thigh was used to quantify muscle area, total fat area, low-density lean tissue area, and muscle attenuation in Hounsfield units (HU) of both the right and left legs ([22]).
All subjects were weight stabilized (±2%) for at least 2 weeks before metabolic testing before and after the interventions and were provided with a eucaloric diet for 2 days before the clamp by a RD to control nutrient intake ([22]). All testing was performed in the morning after a 12-h overnight fast. At the end of the 6-month program, subjects were asked to continue the aerobic training 3 days/week during the final testing period, and the glucose clamps were performed 36-48 h after the last bout of exercise.
Blood samples were drawn before and at 30-min intervals for 2 h after ingestion of 75 g of glucose. Plasma glucose concentrations were measured using the glucose oxidase method (2300 STAT Plus, YSI, Yellow Springs, OH). Plasma insulin was measured by radioimmunoassay (RIA) (Linco Research, St. Charles, MO). Glucose and insulin total area under the curve (GAUC and IAUC, respectively) were calculated by the trapezoidal method.
Peripheral tissue sensitivity to exogenous insulin was measured using the hyperinsulinemic-euglycemic clamp technique ([23]). Arterialized blood was obtained from a dorsal heated hand vein ([24]). For the assessment of basal glucose and insulin levels, three arterialized blood samples were drawn at 10-min intervals. Blood samples were obtained every 5 and 10 min thereafter for the determination of plasma glucose and insulin levels. A 10 min priming and continuous infusion of insulin (80 mU m−2 min−1, Humulin, Eli Lilly Co., Indianapolis, IN) was performed for 180 min with a continuous infusion of 20% glucose solution starting at 10 min. The plasma glucose levels during each clamp period (10-180 min) did not differ before and after the interventions and averaged 5.13 ± 0.06 vs. 5.10 ± 0.07 mmol/L, which was 95 ± 0.8% of the desired goal with a coefficient of variation of 5.9 ± 0.3% with insulin levels of 1,062 ± 26 pmol/L in all clamps (n = 64). One male did not undergo the glucose clamp procedure.
Blood samples were collected for serum or plasma (heparin) by blood draw and placed in test tubes. For the serum samples, blood was allowed to clot by leaving it undisturbed at room temperature for 30 min. For the plasma, samples were placed in prechilled test tubes containing 1.5 mg EDTA/mL of blood and centrifuged at 2,000g for 10 min at 4°C. The liquid component (serum or plasma) was immediately transferred into clean polypropylene tubes. Aliquots were stored at −80°C until analysis for plasma insulin by RIA ([22]) and for serum myostatin by enzyme-linked immunosorbent assay (ELISA). Serum myostatin was measured in duplicate using Myostatin ELISA kit according to the manufacturer's instructions (ALPCO, Cat# K1012). Samples were read using VERSAMAX microtiter plate reader at 450 nm against 620 nm as a reference. A 4-parameter-algorithm was used to calculate the standard curve and the concentrations of myostatin in human serum were determined. The coefficient of variance between replicates was below 15%.
Before the start of the clamp procedure and at 120-min during the glucose clamp, vastus lateralis muscle biopsies were taken from each subject under local anesthesia using a 5 mm Bergstrom needle for the measurement of basal and insulin-stimulated gene expression. Three women had basal and not insulin-stimulated muscle biopsies. Muscle was immediately freeze-clamped and stored at −80°C. Approximately 50-80 mg of muscle was used for RNA isolation and 100 mg of muscle was used for tissue lysate preparation. There was sufficient muscle sample of 15 individuals for the western blot analyses.
Total RNA was extracted from skeletal muscle using TRIzol® Reagent (Invitrogen, Cat# 15596-018) as specified by the manufacturer. The RNA pellet was resuspended in RNAsecure Resuspension solution (Cat# 7010, Ambion) and RNA concentrations were measured in a spectrophotometer.
One microgram of total RNA for each sample was reverse transcripted into first strand cDNA using Transcriptor First Strand cDNA Synthesis Kit (Cat# 04 896 866 001, Roche Applied Science) according to the detailed manufacturer's protocol. The random primer was used as the primer and the RT reaction was carried out at 10 min at 25°C and then 55°C for 30 min in 20 μl volume. The reaction was inactivated by incubating at 85°C for 10 min and stopped by placing the tube on ice. An RT control (master mix without RT enzyme) was performed.
Quantitative real-time PCR (qPCR) and data analysis were performed in a LightCycler 480 Real-Time PCR System with LightCycler® 480 software (Roche Applied Science). LightCycler 480 Multiwell plate 384 (Cat# 04 729 748 001), LightCycler 480 Probes Master kit (Cat# 04 887 301 001), and Taqman gene expression primer/probe set (Applied Biosystems, Foster City, CA) were used. Each qPCR reaction was carried out in a final volume of 10 μl, consisting of 2 μl 1:4 diluted template cDNA, 5 μl LightCycler 480 Probes Master, 0.5 μl Taqman gene expression primer and probe mix, and 2.5 μl nuclease-free water. Water instead of cDNA served as the no template control. According to the manufacturer's instruction, the qPCR protocol was adopted for all samples: after incubation at 95°C for 10 min to activate the DNA polymerase, 45 cycles of 95°C for 10 s and 60°C for 30 s each were performed to facilitate the PCR reaction. 36B4 served as an internal control for normalization. Data acquisition occurred at real time during the annealing/elongation incubation at 60°C. All samples were amplified in triplicate from the same RNA preparation. Gene expression data were analyzed by Roche LightCycle 480 system Software version 1.5 advanced relative quantification program. Data is presented as the mean ± SEM of three determinations for each sample and the normalized ratio of Target/Reference.
In a 15 individuals (n = 7 women and n = 8 men), a fasting/basal muscle samples obtained before and after AEX + WL were lyophilized for 48 h and then dissected free of obvious connective tissue, fat, and blood. Microdissected muscle (∼5 mg) was homogenized in complete RIPA buffer (Santa Cruz Cat# sc-24948) for 1 min on ice. The homogenate was incubated on ice with shaking for 30 min and then centrifuged at 14,000 rpm for 15 min at 4°C. Total protein concentration was determined using bicinchoninic acid (BCA) protein assay kit (Pierce Cat# 23227). Thirty micrograms of protein were denatured at 100°C for 5 min and separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel (Bio-Rad Cat# 345-0009) along with molecular weight markers and subsequently transferred to polyvinylidene difluoride (PVDF) membranes. The membrane was blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween 20 (TBS-T) for 1 h at room temperature, then incubated overnight at 4°C with primary antibodies (MSTN, NVUS Cat# H00002660-M07 and GAPDH, Santa Cruz, Cat# sc-25778) diluted according to the manufacturer. After TBS-T for 15 min 3× washes, the membranes were incubated for 1 h at room temperature with secondary antibody, anti-mouse IgG TrueBlot conjugated with horseradish peroxidase (HRP) (eBioscience, Cat# 18-8817), and goat anti-rabbit IgG conjugated with HRP. Detection was performed using the ECL Plus Western Blotting Detection System (GE Healthcare, Cat# PRN2132). The emitted light was captured on X-OMATTM Blue XB film (Kodak, Cat# 1776699). Blots were imaged using a personal density scanning imager (Molecular Dynamics), and protein band densities were determined using the Image QuaNT software 5.0 (Molecular Dynamics). GAPDH served as an internal reference and was used to correct the difference in sample concentration and loadings. The whole normal skeletal muscle lysate (NUVS Cat# NB820-59169) served as a positive control and an external calibrator to counteract variations in Western blotting efficiency. Target/reference ratio was normalized using the calibrator on each Blot.
For the hyperinsulinemic-euglycemic clamps, the mean concentrations of glucose and insulin were calculated for each sample time point. The trapezoidal rule was used to calculate the integrated response over 30-min intervals from 30-180 min for each subject during the clamp. The integrated response was divided by its time interval to compute mean concentrations for glucose and insulin during the clamp. Glucose utilization (M) for 30-min intervals was calculated from the amount of glucose infused after correction for glucose equivalent space (glucose space correction). For one male who did not undergo post DXA, the FFM change was estimated from the mean change in order to calculate M expressed per FFM. The CT mid-thigh measurements were not different between the right and left legs so the values of the right leg were used in the statistical analyses.
Differences between pre-intervention and post-intervention measures of variables were determined using a paired t-test. Univariate regression analyses were used to determine predictors of glucose utilization. Statistical significance was set at P < 0.05. Data were analyzed by SPSS statistical software (SPSS, Chicago) and expressed as mean ± SEM.
Body weight decreased 8% (P < 0.001) accompanied by an absolute decrease in percent body fat of 4% (P < 0.001), a 17% decrease in total fat mass (P < 0.001), and no change in FFM or mid-thigh muscle area (Table 1). After AEX + WL, mid-thigh subcutaneous fat decreased (P < 0.001), low-density lean tissue area decreased (P < 0.05), and muscle attenuation increased (P < 0.05). VO2max (L/min) increased 14% after AEX + WL (P < 0.001).
|Age, y
|61 ± 1 years
|Weight, kg
|90.8 ± 2.8
|83.7 ± 2.7a
|BMI, kg/m2
|31.2 ± 0.7
|28.3 ± 0.7a
|% Body fat
|39.2 ± 1.6
|34.8 ± 1.8a
|Fat mass, kg
|35.5 ± 1.9
|29.3 ± 1.8a
|Fat-free mass, kg
|55.4 ± 2.3
|54.9 ± 2.3
|VO2max, L/min
|2.00 ± 0.11
|2.26 ± 0.11a
|Visceral fat area, cm2
|163.0 ± 16.9
|137.8 ± 14.1b
|Subcutaneous abdominal fat, cm2
|357.5 ± 23.5
|306.8 ± 24.8a
|Mid-thigh muscle area, cm2
|90.7 ± 6.1
|92.6 ± 5.2
|Mid-thigh subcutaneous fat, cm2
|118.7 ± 11.6
|102.2 ± 9.6a
|Mid-thigh low density lean tissue, cm2
|22.9 ± 1.5
|21.5 ± 1.6c
|Mid-thigh muscle attenuation (HU)
|36.2 ± 2.6
|38.8 ± 3.0c
|Fasting plasma glucose, mmol/L
|5.4 ± 0.1
|5.2 ± 0.1
|Fasting plasma insulin, pmol/L
|87 ± 7
|69 ± 5a
|Glucose AUC, mmol/L/min
|1,271 ± 51
|1,212 ± 42c
|Insulin AUC, pmol/L/min
|68,607 ± 5,499
|57,127 ± 4,521b
|M, μmol min−1
|48.0 ± 3.29
|56.5 ± 2.9b
At baseline, 19 subjects had normal glucose tolerance, 11 had impaired glucose tolerance, and 3 had unconfirmed diabetes ([25]). Fasting plasma glucose concentrations did not change after AEX + WL but fasting insulin levels were reduced (P < 0.001, Table 1). Glucose AUC during the OGTT decreased 5% (P < 0.05) and insulin AUC during the OGTT decreased 17% (P < 0.01). M increased 18% (P < 0.001) after AEX + WL.
Basal myostatin mRNA levels decreased 19% after AEX + WL (83.0 ± 9.7 vs. 67.5 ± 6.4 AU, P < 0.05, Figure 1) but myostatin protein levels did not change significantly for 26-kDa (1.29 ± 0.10 vs. 1.34 ± 0.10 AU) and 50-kDa (1.44 ± 0.16 vs. 1.26 ± 0.11 AU; Figure 2). Basal myostatin mRNA levels were negatively associated with M before the intervention (r = −0.43, P < 0.05, Figure 3). There was a trend toward a difference in the change in myostatin mRNA levels with insulin stimulation before vs. after AEX + WL (−7.2 ± 4.7 vs. 6.0 ± 7.5 AU, P = 0.10). The insulin effect on myostatin gene expression was associated with the change in M after AEX + WL (r = 0.56, P < 0.005). Serum myostatin tended to decrease after AEX + WL (7.25 ± 3.71 vs. 6.10 ± 2.44 ng/mL, P = 0.07). In an examination of each gender, we observed a decrease in skeletal muscle 50-kDa myostatin protein levels in women (1.75 vs. 1.22 AU, P < 0.05) and a decrease in serum myostatin in men (9.02 vs. 6.48 ng/mL, P < 0.05). There were no associations between age and BMI and changes in skeletal muscle protein or gene expression.
Basal MyoD (2.59 ± 0.11 vs. 2.60 ± 0.09 AU) and myogenin (2.39 ± 0.09 vs. 2.39 ± 0.10 AU) mRNA levels did not change significantly after AEX+WL. However, insulin infusion increased myoD mRNA before (+2.62 ± 0.19 AU) and after (+2.71 ± 0.25 AU) AEX+WL (both P < 0.001). Likewise, hyperinsulinemia increased myogenin gene expression before and after AEX + WL (+0.71 ± 0.09 and +0.84 ± 0.16 AU, respectively, both P < 0.001). The increase in myoD and myogenin expression during hyperinsulinemia was similar before and after AEX + WL.
Our results indicate that a downregulation of skeletal muscle mRNA content of myostatin occurs after an AEX + WL program in obese men and women. Basal MyoD and myogenin mRNA and myostatin protein expression did not significantly change after the intervention.
Satellite cells are important in the mechanisms involved in the muscles' response to exercise training. Once the cells are activated, they proliferate and fuse together with pre-existing fibers to regenerate muscle tissue. Activated satellite cells can express the myogenic factors including MyoD, myogenin, and myostatin. Myostatin, a negative regulator of muscle cell growth, represses myoD and myogenin protein expression ([26]). Because of the importance of these genes in muscle growth, it is conceivable that an exercise training program could modify the expression of these genes.
To our knowledge, there are no studies examining the effects of aerobic training and weight loss on skeletal muscle basal myostatin, MyoD, or myogenin levels in older men and women. We found a significant decrease in myostatin gene expression after AEX + WL. Although we found a 10% decrease in 50-kDa myostatin protein levels, this was not significant but is similar in magnitude to the 12% decrease in myostatin gene expression. We did not observe any significant changes in 26-kDa myostain protein, MyoD or myogenin mRNA expression after AEX + WL. Others report downregulation of myostatin mRNA without changing myostatin protein levels in conditions such as sepsis in rodents ([27]). A 6-month aerobic training program in 10 middle-aged men resulted in a decrease in myostatin protein levels ([13]). An acute cycling bout increased mRNA myostatin by 2-fold, MyoD by 3-fold, and myogenin by <1-fold in endurance trained subjects ([28]). The significant decrease in basal myostatin mRNA after the AEX + WL in our study is consistent with studies using resistive exercise (RT) as the mode of training whereby acute bouts of resistive exercise ([6]) and 9 weeks of RT in older men ([11]) reduce muscle myostatin mRNA. We have also observed a significant decrease in myostatin mRNA expression in the paretic muscle of stroke survivors after 12 weeks of high intensity RT ([12]). One study has reported an increase in myostatin expression after an acute resistive session ([29]). In addition, an acute bout of RT can reduce ([6]) or not change ([8]) myogenin levels and increase MyoD mRNA expression ([30]) in young but not elderly subjects ([31]). Although MyoD and myogenin gene expression increased after 6 weeks of RT in young males ([32]), we found no change in MyoD or myogenin after a longer aerobic exercise program.
Recent papers ([17, 18]) provide evidence that myostatin knockout mice have increased insulin sensitivity and glucose uptake. Mstn−/− mice have increased muscle mass, lower adipose tissue mass, and lower basal skeletal muscle, and whole body lipid oxidation with improved insulin signaling by increased Akt phosphorylation during insulin ([17]). Myostatin mice with the loss of function of both alleles (MstnLn/Ln) have improved glucose disposal rate compared with Mstn+/Ln on a high-fat diet ([18]). In addition, TNFα expression is lower in both muscle and adipose tissue and treatment with recombinant myostatin increases TNFα and results in insulin resistance ([18]). Treatment of mice with a soluble activin receptor type IIB (an endogenous signaling receptor for myostatin) suppresses hepatic glucose production and increases glucose uptake ([33]). These animal models provide clear mechanistic evidence of the detrimental effects of myostatin on insulin sensitivity. We are the first to provide human evidence that skeletal muscle myostatin mRNA may be associated with insulin resistance in obese adults although circulating myostatin levels have been examined ([13]). Our results are consistent with a study in middle-aged men whereby plasma myostatin protein levels were inversely correlated with insulin sensitivity measured during an intravenous glucose tolerance test ([13]). Some potential molecular mechanisms responsible for increased insulin sensitivity in myostatin knock-out mice are increased AMP-activated protein kinase activity in skeletal muscle, increased Glut4 levels, Akt and phosphorylated Akt ([19]). Treatment of human myoblasts with myostatin for 24 h decreases the phosphorylation of Akt by ∼50% ([34]). During insulin stimulation in vivo, p-Akt increases in adults as a mechanism for increased insulin sensitivity ([20]), which could suggest that myostatin might change during hyperinsulinemia. Our results also show that the insulin effect on myostatin gene expression was associated with the change in insulin sensitivity after the intervention suggesting that those individuals with the greatest change in myostatin mRNA during hyperinsulinemia had the greatest improvement in insulin sensitivity after AEX + WL. Thus, alterations in insulin signaling may be one mechanistic link with changes in myostatin during hyperinsulinemia in humans, although this cannot be addressed in the present investigation. Furthermore, myostatin-knock out mice have an increase in carbohydrate utilization for energy and increase in respiratory exchange ratio ([17]). Together, these would also suggest that the increased carbohydrate oxidation and decrease in fat oxidation during a clamp ([22, 35]) would align with a potential change in myostatin during insulin. More work is needed to further advance our understanding of myostatin's role in insulin action in vivo.
There are a several limitations in our study that deserve discussion. First, we recognize that our study cannot distinguish whether aerobic exercise alone (described above in one study) or the weight loss contributed to the changes in myostatin that we observed. There are only two papers that have examined the effects of weight loss on myostatin ([14, 15]) but neither examined MyoD or myogenin. Both groups of investigators measured myostatin gene expression before and after severe weight loss induced by gastric bypass surgery (body weight decreased by 38% in n = 6 and 45% in n = 3) in morbidly obese subjects. They reported significant reductions in myostatin mRNA levels ([14, 15]). Given the large degree of weight loss in these two studies, it is unknown whether the modest (8%) decrease in weight in our current study would have such an effect on myostatin. Additional studies of weight loss alone and aerobic exercise training alone on myostatin would be valuable. Although we observed a 10% decrease in myostatin protein levels, the reduced sample size in this measure could have limited our ability to detect a statistical significant change after AEX + WL. In addition, our study results are only applicable to older overweight and obese adults.
We found that myostatin mRNA expression is significantly reduced concomitant with improved insulin sensitivity after a 6-month aerobic exercise training program combined with modest weight loss in obese adults. Further studies are necessary to directly address changes in fuel utilization and the relationship to myostatin signaling. The role of exercise training and myostatin inhibition in the mechanism for improvements in insulin sensitivity also warrants additional investigation.
Our appreciation is extended to the women and men who participated in this study. We are grateful to Lyndon J. Joseph, PhD, Andrew P. Goldberg, MD, Leslie Katzel, MD, PhD, the nurses, dieticians, research assistants Melissa Gray, Carol St. Clair, Lynda Robey and Gretchen Zietowski, of the Division of Gerontology and GRECC for their assistance to this project. This study was supported by funds from: the Baltimore VA Medical Research Service, VA Research Career Scientist Award (ASR), VA Merit Award, VA Career Development Award (JB), Department of Veterans Affairs and Veterans Affairs Medical Center Baltimore Geriatric Research, Education and Clinical Center (GRECC), NIH grants (RO1-AG19310, K01 AG021457, P30 AG028747, P30 DK072488, P60 DK079637, and M01 RR016500).


text:
April 12, 2017
See beautiful new work created using the fluid simulator, from CG Coca-Cola to Game of Thrones. Read More »
March 28, 2017
See the best new VFX created in Max and Maya: from Deadpool to The Mill's amazing commercials. Read More »
March 19, 2017
See sneak peeks of the Final Fantasy VII remake, Injustice 2 and other new titles created in UE4. Read More »
February 26, 2017
Two minutes of the best games work created in Houdini, from Uncharted 4 to amazing cinematics. Read More »
February 24, 2017
Watch gorgeous games graphics from indie hits like Firewatch to Blur Studio's Halo Wars 2 cinematic. Read More »
January 19, 2017
See amazing work being created with the Maya crowd simulator, including epic VFX from Ant-Man and San Andreas. Read More »
November 18, 2016
See the best new work created using Maya, Max and Flame - from Hollywood movies to Korean ads. Read More »
September 13, 2016
See the best new work created using the open-source 3D app, from cartoon shorts to photorealistic VFX. Read More »
August 1, 2016
See amazing movie shots created with the digital vegetation tool, from Avatar to The Force Awakens. Read More »
July 31, 2016
See the year's best motion design and movie UI work, from top studios like Territory and ManvsMachine. Read More »
July 31, 2016
See four minutes of the best VFX and animation created in Houdini, from San Andreas to Dawn of War III. Read More »
May 24, 2016
See two minutes of amazing motion design work from folks like Beeple, John Likens and Aixsponza. Read More »
May 11, 2016
Master the 3D texture painting app with the new free learning edition, or use it to create demo reel material. Read More »
April 20, 2016
See adrenaline-pumping VFX work created with Nuke, Mari and Katana - including most of the Oscar long list. Read More »
March 24, 2016
See the best new work created with Substance Designer and Painter: from personal stills to AAA titles like Uncharted 4. Read More »
March 18, 2016
Video: Beautiful new reel shows particle effects and character animation rendered using the real-time viewer. Read More »
February 2, 2016
See three minutes of gorgeous new work created with the fluid simulator, from ads to the titles for Daredevil. Read More »
January 15, 2016
See nice things created with the help of the shot review tool, including VFX from Mad Max and Jurassic World. Read More »
December 1, 2015 | 1 comment
See the best new projects created using the 3D clothing design tool - from Ted 2 to Metal Gear Solid V. Read More »
November 12, 2015
Orcs, dragons, Turtles, Terminators, Minions and Avengers assemble to bring you ZBrush's greatest CG hits. Read More »
Sorry, no posts matched your criteria.