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Throw a Spongebob Themed Party
If you are looking for a great idea for your child's birthday, consider throwing a cartoon themed party. You can find plenty of decorations for all popular cartoons and the party will sure be a hit.
For example, consider Spongebob Squarepants. Spongebob is one of the most popular cartoon characters that appeals to kids from little to big. He is funny, witty and has a cast of friends that everybody loves.
For your party, you can easily decorate the location with Spongebob related posters, balloons and table sets. Wilton sells Spongebob shaped cake baking pan that will make creating a custom cake a breeze.
During the party, find some fun games or pull up some creative fun short Youtube videos for kids. Here is one that everybody is going to love:
SpongeBob Squarepants
Parenting Advice You Need To Remember
A lot of times, the only training parents get is the training they get "on the job". If you want to improve your parenting skills, check out some of the following tips and advice. As with anything, the better informed you are, the better you will be. That applies to raising a child as well.
If you are an expecting mother, don't hurt your checkbook by spending money on expensive nursery equipment. It is always possible to buy baby furniture, bedding and the like at much better prices by shopping at large discount stores. A lot of people give away their baby goods when they are done using them.
If you take your child on a trip, keep routines as similar as possible by eating and sleeping in the same way, at around the same times as normal. Infants and children are often very stressed by the disruption of schedules traveling causes. Having their normal rituals in place makes the new space seem less scary and more relaxing, which will help everyone get to sleep.
Don't let a kid younger than four drink any sort of soda. Stick with drinks which provide your child nutrients like water, juice or milk.
Any child who walks to classes each day ought to have reflective fabric on their clothes or book bags. Velcro even makes detachable reflective strips specifically for this purpose. In the winter months when light is scant, reflective tape or strips increase the visibility of your child to drivers.
You should never smoke indoors when you have children. You should even think about completely quitting smoking. The dangers of inhaling secondhand smoke are the same as those of smoking. When children inhale second-hand cigarette smoke, they are more apt to develop medical problem, such as pneumonia or asthma.
Setting concise ground rules for behavior that are enforced with polite
phrases can work well at harmonizing your family. For example, instead of "no hitting," opt for "only touch gently."
If you want to avoid getting your children too cranky and bored, then periodically make travel stops. The longer distance that you travel, the more stops you should make. Your natural temptation might be to hustle through the journey as fast as you can, but a relaxed pace, with plenty of pauses, will cut down on backseat squabbling. To give your children a chance table and chair rental Atlanta GA to use some of their excess energy, stop at parks or restaurants that have play areas.
Toddlers often have more toys than they can possibly play with. Rotate them in and out of their toy box to keep them new and interesting. The novelty of a new toy wears off quickly if it is not a favorite, especially for young children. It is your job to keep things fresh in your toddler's play area by incorporating new and fun things to maximize their enjoyment.
It is important to realize that stepchildren do not always like their new stepparent right away. If you are becoming a stepparent soon, it is important to keep this in your thoughts at all times. It is natural if they feel resentment toward you over the fact that their mom and dad are no longer together. It is important to take the relationship slow. It will allow you and the child to get to know each other better and to have a relationship that is built on a strong foundation.
As the article above stated, being a parent is a process and is not just about letting things happen naturally. Use the information presented in this article to develop new strategies and techniques that will allow you to become a better parent.
Children Party Tips and Ideas
No matter what the occasion you are planning for, whether it is a Birthday celebration a fund raiser for your charity, or your annual summer season party, you are going to be searching for some sort of entertainment.
Magicians and children's performers will do magic and balloon modeling that can provide your group a form of home entertainment.
Bear in mind that the birthday individual is the most important person so involve them in gathering birthday celebration ideas. After all, you are commemorating the birth and life of a person you care about, so why not make their event something they will like.
All parents know that their children's birthday parties are incredibly important. Not just for the child, but for you too. Every moms and dad tries to outdo each other and have the very best party for their kid. Sadly, all this pressure typically avoids the parents from offering their child the party they desire or are worthy of. Attempting to come up with a brand-new and uncommon idea can be very tough and the kid can feel that their wishes are not being thought about at all. The answer could be to have a style for your party, something that your daughter or son wants.
Party themes have such a huge capacity in making celebrations enjoyable since they focus the party around a specific idea or idea. Starting with terrific celebration themes allows you to have details such as food, decorations and video games to all center around the same celebration concept. What fun it can be to reach a party and see that everything has actually been prepared and finished with intention.
Think about all the parties you have gone to and exactly what protrudes from them? If they were exciting and fun, why? I think among the best ways to make a distinct and genuinely fun celebration is to have a kids's performer who is various and distinct and who will supply new and different parlor game.
Regretfully a big quantity of food can in some cases go to waste. Ideally planning to supplying lots of beverages for the children in a quickly non reusable method. Children always go for quick finger food at celebrations and they like enjoyable vibrant food.
Great Corporate Party Ideas To Please Employees
Preparing for a corporate party is no simple task. Sure, you could just put on some music, serve party foods, and decorate the room with balloons. However attending yet another common festivity will only seem repetitive and maybe even boring to your boss and employees. The best way to celebrate any occasion such as a retirement party, a roast of the Ceo, or a any other special occasion is with one of a kind company party ideas and themes.
One benefit of planning a party at the workplace is that a small budget typically is not an concern. Employers often like to treat their employees during company events to make them an event to look forward to. The purpose of a corporate get togeth
Halloween Health and Safety Tips
Fall celebrations like Halloween and Harvest Day are fun times for children, who can dress up in costumes, enjoy parties, and eat yummy treats. These celebrations also provide a chance to give out healthy snacks, get physical activity, and focus on safety.
Check out these tips to help make the festivities fun and safe for trick-or-treaters and party guests.
Going trick-or-treating?
Swords, knives, and other costume accessories should be short, soft, and flexible.
Avoid trick-or-treating alone. Walk in groups or with a trusted adult.
Fasten reflective tape to costumes and bags to help drivers see you.
Examine all treats for choking hazards and tampering before eating them. Limit the amount of treats you eat.
Hold a flashlight while trick-or-treating to help you see and others see you. WALK and don't run from house to house.
Always test make-up in a small area first. Remove it before bedtime to prevent possible skin and eye irritation.
Look both ways before crossing the street. Use crosswalks wherever possible.
Lower your risk for serious eye injury by not wearing decorative contact lenses.
Only walk on sidewalks whenever possible, or on the far edge of the road facing traffic to stay safe.
Wear well-fitting masks, costumes, and shoes to avoid blocked vision, trips, and falls.
Eat only factory-wrapped treats. Avoid eating homemade treats made by strangers.
Enter homes only if you're with a trusted adult. Only visit well-lit houses. Never accept rides from strangers.
Never walk near lit candles or luminaries. Be sure to wear flame-resistant costumes.
More Halloween Safety Tips
Halloween is an exciting time of year for kids, and to help ensure they have a safe holiday, here are some tips from the American Academy of Pediatrics (AAP).
* Plan costumes that are bright and reflective. Make sure that shoes fit well and that costumes are short enough to prevent tripping, entanglement or contact with flame.
* Consider adding reflective tape or striping to costumes and trick-or-treat bags for greater visibility.
* Because masks can limit or block eyesight, consider non-toxic
Planning Suggestions for Summer Weddings, Company Picnics and Family Reunions
When preparing summer month weddings, business picnics and family reunions, it is essential that you water slide rentals Richmond TX consider certain pointers for the celebration. This is because without those ideas the celebration or event that you are preparing may fail to kick off. The tips that you need to think about for the party are:
Tents, tables and chairs
When you invite|welcome individuals over for a picnic or reunion, you need to make sure that the chairs and tables are enough for everyone. If some individuals get to the venue late and discover that there are no chairs or tables, they may feel offended. Make a rough quote of all the guests you anticipate to have so that you can have the ideal variety of chairs and tables. When you guarantee that there is order in the seating arrangement, then you can place name sticker labels on the chairs if it is a wedding event. This will make sure that just those who had actually been accounted for get the seats.
When individuals go to parties or events, the majority of them expect that they will get to delight in some meal throughout the event. Because of this, it is very important that your guests have adequate food for the party. Your guests will take pleasure in the many concessions that are available. Kids will love the snow cones and cotton candy that will be offered. Consider renting snow cone devices with an assortment of tastes offered. The food needs to be fresh and put in clean containers. If the plates and glasses that are made use of have any dirt or dust, this may leave a very unfavorable impression on your visitors.
Slides and Bounce Houses
Summer season weddings are typically very vibrant and lively. If you are holding the reception in the backyard or in the park, then you need to consider having slides and bounce houses. The bounce house will be utilized by the kids who can spend hours jumping up and down in the castle. This will keep them busy and they will not disrupt their parents during the whole event. The slides will add color and life to the wedding event.
On Site Assistant
Whether it is a company picnic, summer wedding event or family reunion it is essential that you have somebody who understands the inflatables or any other equipment that may need operation throughout the event. The on site attendant can assist in organizing the children and in case there is an issue they can always be sought advice from.


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As new medications become available and the population ages, Americans are using more prescription drugs than ever.
You may worry about side effects, drug interactions, and costs, especially if you have multiple conditions and medications.
Group Health researchers are studying the harmful and beneficial effects of medications. And they are working on ways to manage and monitor drug use safely.
We can dramatically improve health care by making sure people get safe, effective medicines at appropriate doses. We contribute to this effort nationally by developing methods for monitoring medication safety. At the personal level, we're providing information to doctors and patients for weighing the risks and benefits of using prescription drugs.
Our research includes the Centers for Disease Control and Prevention, the US Food and Drug Administration (FDA), the Agency for Healthcare Research and Quality, the Group Health Foundation, and other private sources.
- Helping develop and evaluate a primary care program for safe use of prescription opioids commonly used for pain, such as Oxycontin and Vicodin
- Studying the safety of drugs for cardiovascular conditions, including in older adults
- Proactive monitoring of medical products through the FDA Sentinel Initiative
- Exploring how genetics might personalize treatments
- Studying the safety of medications, including sulfonamide antibiotics during pregnancy
- Investigating whether prescription opioids increase the risk of cognitive decline and dementia in late life
Our work gives patients and providers evidence about the outcomes of medication use—both harmful and beneficial—to help them make informed personal decisions about prescription drug use.
Clinical trials are sets of tests in clinical research and drug development that generate safety and efficiency in data (or more specifically, information about adverse drug reactions and adverse effects of other treatments) for health interventions (e.g., drugs, diagnostics, devices, therapy protocols). They are conducted only after satisfactory information has been gathered on the quality of the nonclinical safety, and health authority/ethics committee approval is granted in the country where approval of the drug or device is sought.
Clinical research is about finding the best way for patients to receive medical care. The NIH defines clinical research in three parts: a) patient-oriented (research involving interaction with human subjects), b) epidemiologic and behavioral, and c) outcomes and health services research.Picture of Laboratory Assistant In each of these cases, researchers are looking to study how to improve health.
Clinical research includes clinical trials, which are studies that evaluate the effectiveness and safety of medical devices or drugs. These studies are conducted according to a formal protocol, which defines what kind of patient can participate, when and how much treatment is given, what is being measured, and the overall duration of the study.
Clinical trials progress in phases, moving toward use for patient care after the drug or device has been thoroughly studied for effectiveness and safety. The phases of clinical trials, which each new treatment must pass to be introduced into the market include:
PHASE I: Researchers test a new drug or treatment in a small group of people for the first time to evaluate its safety, determine a safe dosage range, and identify side effects.
(healthy volunteers)
PHASE II: The drug or treatment is given to a larger group of people to see if it is effective and to further evaluate its safety.
PHASE III: The drug or treatment is given to large groups of people to confirm its effectiveness, monitor side effects, compare it to commonly used treatments, and collect information that will allow the drug or treatment to be used safely.
PHASE IV: Studies are done after the drug or treatment has been marketed to gather information on the drug's effect in various populations and any side effects associated with long-term use.
There are numerous research designs used for clinical research, including descriptive, exploratory, and experimental studies.
Descriptive Research-Descriptive research makes observations about patients or health-related conditions. Usually, this research includes a small sample of patients and intensively studies them to gain insight into the subject of interest. Examples include case-studies, qualitative research, or surveys.
Exploratory Research-Exploratory research examines patients or conditions that have not been extensively studied. Exploratory research looks to clarify or define a problem. Often, relationships or associations are looked for in order to better understand a disease or disorder. For instance, a study may discover a relationship between lifestyle factors and a particular disease. Examples include case-control studies, pilot studies, cohort studies, and historical research.
Experimental Research-Experimental research involves comparing two groups of people. Generally, one of the groups receives an intervention or treatment and the other does not. The purpose of this research is to examine the effect of the intervention. These designs, when randomization is used, are viewed as the "gold standard" in clinical research because the design allows researchers to rule out most, if not all, alternative explanations regarding their results. For example, a researcher may compare a group of patients receiving a placebo drug versus a group receiving an experimental drug. If the two groups are similar at the outset of the study, any differences at the conclusion of the study (e.g., improvement in the disease or disorder in the experimental group) can be attributed to the experimental drug.

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Epigenetic mechanisms have been ascribed important roles in endometriosis. Covalent histone modifications at lysine residues have been shown to regulate gene expression and thus contribute to pathological states in many diseases. In endometriosis, histone deacetylase inhibition (HDACi) resulted in reactivation of E-cadherin, attenuation of invasion, decreased proliferation of endometriotic cells, and caused lesion regression in an animal model. This study was conducted to assess basal and hormone-regulated gene expression levels of HDAC1 and HDAC2 (HDAC1/2) in cell lines and protein expression levels in tissues. Basal and steroid hormone-regulated HDAC1/2 gene expression levels were determined by quantitative polymerase chain reaction in cell lines and tissues. Protein levels were measured by immunohistochemistry (IHC) in tissues on an endometriosis tissue microarray (TMA). Basal HDAC1/2 gene expression levels were significantly higher in endometriotic versus endometrial stromal cells, which was confirmed by Western blot analysis. Estradiol (E2) and progesterone (P4) significantly downregulated HDAC1 expression in endometrial epithelial cells. Levels of HDAC2 were upregulated by E2 and downregulated by E2 + P4 in endometrial stromal cells. Hormone modulation of HDAC1/2 gene expression was lost in the endometriotic cell line. Immunohistochemistry showed that HDAC1/2 proteins were expressed in a substantial proportion of lesions and endometrium from patients, and their expression levels varied according to lesion localization. The highest proportion of strong HDAC1 immunostaining was seen in ovarian, skin, and gastrointestinal lesions, and of HDAC2 in skin lesions and endometrium from patients with endometriosis. These studies suggest that endometriosis etiology may be partially explained by epigenetic regulation of gene expression due to dysregulations in the expression of HDACs.
Although the etiology of endometriosis is not completely understood, various theories attempt to explain the mechanisms whereby endometrial cells are able to survive and grow in the peritoneum and other ectopic sites, including genetic predisposition, immune system disturbances, and environmental exposures.1,2 Global gene expression studies in both human and animal models have shown that endometriotic lesions have aberrant gene expression profiles when compared to normal endometrium.3–7 The mechanisms at play have not been sufficiently studied, but epigenetic regulation of gene expression is emerging as a key player in endometriosis.8 Thus, the current knowledge on the pathophysiology of endometriosis supports the involvement of both genetic and epigenetic phenomena in inducing changes in gene expression that lead to disease.
Histone modification, including deacetylation of lysine residues by histone deacetylases (HDACs), is a key mechanism of gene expression regulation in cancer and other diseases.9–11 Histone deacetylases, involved in cell cycle regulation, proliferation, and cell differentiation, covalently acetylate key residues in histones, thus changing chromatin conformation and result in transcriptional repression.12,13 Histone deacetylation, like promoter methylation, generally results in gene silencing; thus HDACs act as transcriptional repressors. The HDAC-related mechanisms also appear to play a role in endometriosis. Recently, Wu et al showed that HDAC inhibition (HDACi) resulted in reactivation of E-cadherin, attenuation of invasion, and decreased proliferation of endometriotic cells.14,15 In vitro, HDACi induced cell cycle arrest by upregulating p21 levels and promoted apoptosis16,17; in vivo, HDACi reduced endometriotic lesion growth and hyperalgesia in a mouse model.18 These data indicate that methylation changes are not the only epigenetic mechanism involved in endometriosis and led to the proposal that HDACi could be a possible new treatment for this condition. However, the HDAC expression profile of endometriotic tissues is yet to be assessed.
We hypothesized that histone modification regulates gene expression in endometriosis and is therefore a potential target for therapy. Since endometriotic lesions are characterized by high endogenous estradiol (E2) levels and show abnormal responses to progesterone (P4),19 we also hypothesized that HDAC expression is differentially regulated in normal endometrium versus endometriosis. We specifically aimed to assess the expression levels of HDAC1/2—regulators of most of the reported changes in histone acetylation levels among Class I HDACs (HDAC1, HDAC2, HDAC3, and HDAC8)—in cell lines and human tissues, and their regulation by ovarian steroid hormones in vitro.20 These data will significantly contribute to the current understanding of endometriosis by dissecting the epigenetic mechanisms at play.
Human endometrial stromal (HESCs) and epithelial endometrial (EEC) cell lines were cultured as described.21,22 The stromal endometriotic cell line Hs832cT (CRL-7566) was obtained from American Type Culture Collection (ATCC; Manassas, Virginia) and cultured following their recommendations. This cell line, derived from an ovarian cyst from a patient with endometriosis, has been partially characterized as stromal (expressing vimentin but not cytokeratin nor E-cadherin). Compared to EEC, Hs832cT cells have a lower ER1/ER2 ratio and lower PR protein levels (Bello et al, unpublished data). Steroid hormone treatments were conducted as described by Ruiz et al.23
Flash-frozen endometriotic lesions and endometrial biopsies were collected from volunteers (19-47 years old) undergoing surgery for benign gynecologic conditions, not on hormonal medication for at least 3 months prior. Average age of patients and controls were 32.9 years (range: 19-47 years) and 42.7 years (range: 40-47 years), respectively (P < .05). Disease severity was determined by collaborating gynecologists using the revised American Fertility Society criteria.24 Frozen tissues (n = 49) were analyzed for pathological diagnosis and macrodissection to exclude nonendometriotic tissue. Tissues analyzed included 15 endometriotic lesions, 11 endometrial biopsies from endometriosis patients, and 23 endometrial biopsies from women without endometriosis. Protocols involving tissue collection were approved by the PSMHS IRB Committee. All participants signed a consent form and completed a demographic and clinical questionnaire.
RNA was extracted following standard procedures (RNAeasy Qiagen, Valencia, California). After deoxyribonuclease treatment, total RNA was reverse transcribed (iScript cDNA synthesis kit, Bio-Rad, Hercules, California), and gene expression levels were determined using TaqMan Gene Expression Assays (HDAC1: Hs00605262_g1; HDAC2: Hs00231032_m1; Applied Biosystems, Foster City, California). Quantitative polymerase chain reaction was performed as described using glyceraldehyde 3-phosphate dehydrogenase (Hs99999905_m1) and beta2M (Hs99999907_m1) for normalization in cells and tissues, respectively.23 Basal gene expression was reported as relative units (RUs; 2−Ct).25
Total protein extraction, quantification, and Western blot analysis were conducted as described in detail by Ruiz et al (2010).23 Polyclonal primary antibodies to HDAC1 and HDAC2 were used at dilutions of 1:500 and 1:5000, respectively. Enhanced chemiluminescence was analyzed on a ChemiDoc XRS+ system (Bio-Rad, Hercules, California).
De-identified formalin-fixed paraffin-embedded endometrium and endometriosis tissues were obtained from a collaborating pathology laboratory after protocol approval by the PSMHS IRB Committee. Average age of patients and controls represented in the TMA were 35 and 42 years, respectively. All biopsies were evaluated by a pathologist (M.G.) who confirmed the diagnosis and stage of the menstrual cycle of eutopic endometrial samples using Noyes criteria.26 A total of 164 core biopsies obtained from 83 tissue blocks were used to construct a TMA at the Moffitt Cancer Center Pathology Department. From most blocks, 2 different core biopsies were included in the TMA. The total number of cores on the TMA were 29 ovarian endometriosis, 16 fallopian tube endometriosis, 34 peritoneal endometriosis, 4 skin (umbilical) endometriosis, 7 gastrointestinal (GI), 22 eutopic endometrium of patients with endometriosis (EE), 14 control proliferative phase endometrium (PE), and 38 control secretory phase endometrium (SE). Control endometrium were obtained from women with uterine myomas, abnormal uterine bleeding, or enlarged/prolapsed uterus.
Ten-micrometer sections of the TMA block were cut for immunostaining. The endometriotic and eutopic endometrial tissue sections on the TMA were stained using the LSAB+ Kit (Dako, Carpinteria, California) following the manufacturer's recommendations. After dewaxing and rehydration, antigen retrieval was conducted with target retrieval solution (pH = 9; Dako) followed by the removal of endogenous peroxidase activity using standard methods. Primary antibodies against HDAC1 (1:250) and HDAC2 (1:100) were incubated in antibody diluents (Dako) for 1 hour at room temperature (RT). Sections were incubated for 30 minutes at RT with the secondary biotinylated antibody developed with diaminobenzidine and counterstained with Meyer hematoxylin solution. Positive controls were sections of tonsils. The slides were mounted and visualized at 10, 20, and ×40 magnification on an inverted microscope with an Olympus 35 mm camera (Nikon, Japan). Immunostaining intensity was blindly scored in nuclei of epithelium, glands, and stroma using a 0 to 3 score system: 0 = no staining, 1 = weak, 2 = moderate, and 3 = strong staining.
Gene expression data are presented as mean RU ± standard deviation (SD). Group variables were compared by Kruskal-Wallis nonparametric analysis of variance (ANOVA). Independent sample Wilcoxon tests were done to assess statistical significance of selected differences between groups. Nonparametric Spearman correlations between gene expression levels of HDAC1/2 were analyzed using GraphPad Prism 4.0 (GraphPad Software Inc, La Jolla, California). Statistical significance of the differences in proportions of tissues on the TMA with strong immunostaining was assessed by 2-sided Fisher Exact test. Statistical significance level was set at P < .05. SPSS15 (SPSS Inc, Chicago, Illinois) was used for the statistical analysis.
Hs832cT cells express higher levels of HDAC1/2 messenger RNA (mRNA) than endometrial cell lines, but these differences were only significant between the endometriotic cells (which are of stromal characteristic) and the endometrial stromal cells (Figure 1). Western blot experiments confirmed that endometriotic cells express higher protein levels of these proteins when compared only to endometrial stromal cells (data not shown). Compared to control (media plus vehicle or CV), E2 + P4 significantly downregulated HDAC1 expression in EEC but not in HESC (Figure 2). Histone deacetylase 2 was significantly upregulated by E2 only in HESC, while E2 + P4 brought levels to those of control. No statistically significant changes in gene expression were observed for Hs832cT.
Mean HDAC1 expression was 10.95 RU (SD ± 6.61; median = 11.14) in lesions, 19.55 RU (SD ± 12.85; median = 15.41) in eutopic endometrium from patients with EE, and 16.18 RU (SD ± 11.87; median = 11.04) in endometrium of women with fibroids (EF; Figure 3). For HDAC2, mean expression was 9.40 RU (SD ± 7.41; median = 5.85) in lesions, 13.69 RU (SD ± 9.23; median = 14.01) in EE, and 6.58 RU (SD ± 3.48; median = 5.23) in EF. Endometriotic lesions expressed the highest mean levels of both HDAC1/2. Across tissues, HDAC1 expression was higher than that of HDAC2. Levels of HDAC1/2 expression were significantly correlated in lesions (Spearman r = .80; 95% CI: 0.41-0.95; P = .002) but not in EE (Spearman r = .43; 95% CI: −0.24 to 0.83; P = .18) nor in endometria from controls (Spearman r = −0.06; 95% CI: −0.47 to 0.38; P = .80).
Nuclear immunostaining for HDAC1/2 was detected in stroma and glands in both diseased and control tissues; however, the intensity of HDAC1/2 protein staining was generally higher in endometriotic lesions compared to eutopic endometrium of controls, with noticeable differences depending on lesion localization (Figure 4). There were no statistically significant differences in mean protein expression of HDAC1/2 in PE versus SE from controls. Expression levels of HDAC1 protein were higher among the endometriotic tissues when compared to PE and SE. In stroma and glands, average HDAC1 immunostaining was lowest in PE, followed by peritoneal lesions and SE. The highest mean expression of HDAC1 was observed in skin, GI, ovarian, and EE. Histone deacetylase 1 staining was significantly stronger in skin lesions in stroma (P = .006) and gland (P = .034) compared to all tissues except for GI lesions. Histone deacetylase 2 protein levels, on the other hand, were lowest in ovarian and highest in skin lesions, followed by EE and GI. In stroma, HDAC2 expression was significantly higher in EE compared to SE (P = .046). Skin lesions expressed higher levels of HDAC2 in both glands and stromal than peritoneal, ovarian, fallopian tubes, and PE (P = .07). Both PE and SE expressed lower levels of HDAC1 than most lesion types, while differences in HDAC2 between diseased and control tissues were subtler.
In skin endometriosis, 100% (4 of 4) of tissues stained strongly for HDAC1 in both stroma and glands. Significantly higher proportions of tissues with strong HDAC1 immunostaining were observed in GI lesions (50% in glands and 50% in stroma) and ovarian lesions (44% in glands and 46% in stroma). Almost half of EE tissues stained strongly for HDAC1 in glands (44%; P = .056) and less in stroma (32%), but this was higher than strongly staining PE (0% in glands and 0% in stroma) and SE (10% in glands and 18% in stroma). The proportion of tissues with strong HDAC2 immunostaining was higher in skin (100% in glands and stroma), followed by peritoneum (32% in glands and 42% in stroma), GI (17% in glands and 43% in stroma), and ovarian (20% in glands and 39% in stroma). A higher proportion of EE had strong expression of HDAC2 in stroma (82%), but not in glands (35%), compared to PE (29% in glands and 57% in stroma) and SE (27% in glands and 40% in stroma; Figures 5 and and66).
Epigenetic mechanisms are emerging as important regulators of gene expression in endometriosis and other inflammatory diseases. This study shows for the first time that (i) levels of gene and protein expression of HDAC1/2 are higher in an endometriotic stromal cell line compared to a normal endometrial stromal cell line, (ii) a substantial proportion of tissues from patients express high mRNA levels of HDAC1/2, (iii) their genes are regulated by ovarian steroid hormones in endometrial cells; and (iv) HDAC1/2 proteins are differentially expressed in endometriotic tissues depending on lesion type/localization.
Histone acetylation and deacetylation, regulated by the counterbalanced action of histone acetyl transferases and HDACs, act in concert to regulate gene expression by changing the conformation of the chromatin.13 Histones in transcriptionally active regions of the genome are generally hyperacetylated. Aberrant HDAC levels or sustained HDAC activity would therefore result in histone deacetylation, tightly packaged chromatin, and downregulation of gene transcription. It has been demonstrated that HDACi can reduce the size of lesions and relieve pain symptoms in mice with experimentally induced endometriosis, suggesting that HDACi could potentially be used as a novel alternative for endometriosis treatment.18 However, there is a need to identify the HDAC isoforms that are relevant in this context and to dissect the specific mechanisms activated in the endometriotic lesions via HDAC expression and function. This understanding will also help explain why endometriotic cells share some molecular mechanisms with cancer cells but do not frequently transform and rarely metastasize.
We also assessed whether gene expression levels of HDAC1/2 are regulated by E2 and/or P4. Histone deacetylase 1 gene expression was significantly downregulated by E2 + P4 in endometrial epithelial cells. In contrast, HDAC2 was upregulated by E2 in stromal cells, while E2 + P4 brought levels back to baseline. Thus, HDAC1/2 expression appears to be downregulated during the secretory phase of the menstrual cycle, when both E2 and P4 levels are elevated, which is consistent with the increase in levels of acetylated H3 and H4 by E2 + P4 in HESCs and EECs.27,28 This pattern of hormonal regulation was lost in the endometriotic cell line. Taken together, these data suggest that due to the known resistance to P4 of endometriotic lesions, downregulation of HDAC1/2 expression is lost leading to increased levels in endometriosis.29 This, in turn, would result in a hypoacetylated state and aberrant gene expression profiles. Ongoing studies in our laboratory are designed to identity genes that are regulated by this mechanism.
Expectedly, HDAC1/2 gene expression levels in fresh frozen human endometriotic tissues were highly heterogeneous. Also, both genes were expressed in a substantial proportion of eutopic endometria from women with endometriosis and of women with uterine fibroids, indicating that aberrant histone deacetylation activity could also play a role in other gynecological diseases. There were no statistically significant differences in the mean expression levels of HDAC1/2 according to menstrual cycle phase, but this study is limited in that normal endometrium samples from healthy volunteers were unavailable for analysis. Interestingly, expression levels of HDAC1/2 were significantly correlated in endometriosis lesions but not in the endometria of women with endometriosis or with other gynecologic diseases. We speculate that HDAC1/2 are co-regulated within the lesions due to their particular inflammatory and hormonal microenvironment.
The observed coexpression and apparent coregulation of HDAC1/2 has important implications in regard to their function as regulators of gene expression.30,31 Histone deacetylase 1/2 are conserved enzymes that are part of the Sin3a repressor complex. Sin3a transcriptionally regulates steroidogenic acute regulatory protein gene, glucocorticoid receptor-mediated transcription, and Snail-mediated E-cadherin repression.32 ,33 Also, HDAC1/2 have overlapping functions in cell cycle regulation and their expression has been correlated with Ki-67 levels.34,35 Their silencing leads to cell cycle arrest and upregulation of the cyclin-dependent kinase inhibitor p21. 36 This correlates with the work by Wu and Guo, showing that HDACi decreased proliferation of an endometriotic cell line by upregulating p21, first uncovering an important role of histone modifications in the pathophysiology of endometriosis.
Eutopic, cycling endometrium has been shown to express Class I HDACs constitutively throughout the menstrual cycle. At the protein level, HDAC2 was found to be slightly elevated during the secretory phase.37 Recently, the profile of global histone acetylation in normal endometrium was assessed.38 In this study, global acetylation was shown to increase when transcription activity is likely to be higher. These data indicate that histone acetylation/deacetylation is hormonally regulated and that abnormal epigenetic modifications may be associated with endometrial pathologies.
We also assessed protein expression levels and cellular localization of HDAC1/2 using an endometriosis TMA. To our knowledge, ours is just the second study of protein expression using this powerful technology. We observed that HDAC1 was highly expressed in skin, GI, and ovarian endometriosis, and less in lesions localized in fallopian tubes or peritoneum; HDAC1 protein expression was also higher in the endometria of patients than controls. Histone deacetylase 2 showed a similar pattern of expression in peritoneal versus ovarian lesions, and it was expressed in a higher proportion of control endometria than HDAC1. It is of great interest to document that not all the lesion types and/or localizations express high levels of HDACs, and that there are statistically significant differences in its expression in glands versus stroma. The relevance of the observed differences in expression related to lesion localization is currently unknown, but we speculate that they may be due to the distinctive etiopathologies of the different lesion types as has been proposed before.39,40
A high degree of heterogeneity in HDAC expression levels has also been described in cancer.41 ,42 Within cancer types the proportion of samples expressing high levels of HDACs ranges from 30% to 95%.41,43 Elevated expression of HDAC1-3 was shown in 61%, 95%, and 83% of endometrial carcinomas and in 61%, 93%, and 84% of ovarian carcinomas, respectively. In ovarian carcinoma, the protein levels of HDAC1-3 increased as lesions progressed from benign to borderline to malignant. Also, levels of expression were stronger in highly proliferative and dedifferentiated colorectal tumors.44 Thus, our results are in agreement with the cancer data in that HDACs expression levels varied considerably among tissue type and localization, and immunostaining was not observed in all tissues studied. Based on our observation that ovarian endometriosis lesions express aberrant HDAC levels compared to peritoneal lesions, together with the knowledge that ovarian endometriosis has been shown to be a risk factor for ovarian cancer,45,46 it can be speculated that epigenetic mechanisms involving histone modifications play an important role in the observed dysregulations of endometriotic cells that, if untreated, may in the long term result in cancer. It would be interesting to correlate the levels of HDAC isoform expression in ovarian lesions with an increased potential for transformation in order to better understand the underlying mechanisms.
This study documents for the first time the expression profile of HDAC1/2 in endometriosis. We showed that HDAC1/2 protein levels vary among lesions, and that a substantial proportion of tissues show aberrant nuclear expression of these enzymes. We speculate that dysregulations in HDAC expression lead to a diseased phenotype characterized by increased cell proliferation and invasion.34 The major limitation of this study is that our comparison group is composed of endometrial tissues from women with uterine fibroids and not women free of any disease. The disadvantage of using such tissues as referent is that we cannot conclusively establish whether healthy endometrium is hypo- or hyper-acetylated as compared to endometriotic tissues. We can only confirm that there are differences in the expression of these genes in 2 different pathological states (a finding that, if validated, could constitute the basis for a diagnostic biomarker). This limitation is due to the fact that our inclusion criteria included laparoscopic confirmation of absence of peritoneal lesions. Since endometriosis requires laparoscopy for diagnosis, women having this type of intervention are unlikely to be free of any other gynecological disease. Despite the limitations of the study, we showed that endometriosis has an epigenetic component that needs to be addressed. Our results should encourage follow-up investigations with tissue free of disease to confirm the role of HDACs in endometriosis.
In summary, these data provide additional evidence in support of a possible role of histone deacetylation in the pathophysiology of endometriosis and other gynecologic diseases and suggest that the use of HDACi as therapeutic agents for endometriosis should take into consideration the localization of lesions and the specific profile of expression of HDAC isoforms, as it is being done for cancer.
MC is sponsored by 1F31 HD065431-01A1, PB by 1F31 HD056964-01A1, and AR by PSM RISE Program (R25 GM082406). We thank Dr Asgerally Fazleabas for kindly providing the human endometrial cell lines (HESC and EEC) and Dr Ed Seto for the Class I HDACs antibodies. We acknowledge the technical support of Lynnette Ruiz, Jessica Castellanos, and Elvin Estrada.
The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: the National Institutes of Health [grants numbers: NIH-NICHD R01-HD050559; ARRA Grant # 3 R01 HD050559-04S1; NIH-MBRS S06-GM08239; NIH-NCI 1U56-CA126379-01; NIH-NIGMS R25 GM082406].
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Interview: Inside Elsphinx's Brain Cave Deluxe
By KC Orcutt - Friday, June 12th, 2015
The mastermind behind Pig Food Records latest release, Elsphinx, introduces Brain Cave Deluxe best himself, saying, "I scattered pieces of myself throughout this like timed land mines. It's designed to entrap you. My breath and brain were entrenched in the making of this record. Long story short, I couldn't be more proud to share it with you."
Much like the earth's rarest solar eclipse, the kind that the news makes a big deal of because it's not going to happen again in our lifetimes and we all must go outside at 2am to look, Elsphinx's record is that level of eclipse rare. It's an absolute beauty. Except the good news is that if the night sky decides to be a cloudy bastard, you're not going to miss out on anything. It's here. This album is extremely special. Now imagine that stay-up-late-in-anticipation was stretched over the past three years, because in the case of Brain Cave Deluxe – it was. That's how long Elsphinx has been in the lab sculpting this monster of a double LP. Three years.
Technically his second project, Elsphinx hasn't remained secluded from the underground rap scene these past three years, with countless features and performances throughout the east coast during these tests and trials of creating what many can agree is a 5-star album. That stay-up-late-anticipation has not disappointed. Filled with sleepless nights, countless hours in the studio, writing and rewriting, adding tracks and taking others away, and essentially just carving away at a project with such passion and skill, that we, as an audience, are truly blessed that it is now ripe enough for us to consume, digest and experience many, many times over.
More importantly, Elsphinx has taught his fans, and also himself, the virtue of patience, perfecting your work and executing creativity on your own terms and timeline. There's a strength behind the album's creative process and even better, the album is just as fresh now as it was when it was first conceived, showing further proof that unique sounds are timeless. That being said, Brain Cave Deluxe is also a massive collaborative effort at its absolute finest, harboring the blood, sweat and tears from much of the Pig Food Records roster and features from other special guests, with Elsphinx in his rightful position as the ringleader to this cosmic rap circus.
Based out of Albany, Elsphinx is hands down making the weirdest rap music to come out of upstate New York, in the best way possible. An artist entirely in his own category, he sets the bar extremely high with this double LP. Going through the 18 tracks, there are a lot of strengths on display with a lot of lyrical content to go through in addition to absolutely spectacular production. This album is a reflection of Elsphinx in the purest form, while still remaining true and humble to his artistry. Elsphinx is here to take listeners on a journey through his eyes, as simple and complex and intriguing as that is.
Brain Cave Deluxe is available through the Pig Food Records site, as well as other digital platforms, and is also released on a special double LP vinyl pressing.
1. Introduce yourself – your name, where you're from & where you're at now.
Greetings. I'm elsphinx. I was born in the panoramic, scenic Adirondack foothills in upstate New York. I lived there long enough to want to get the fuck out. I travelled billions of light years through time and space an hour away to Albany, New York, which I currently inhabit.
2. When did it hit you that you identify with being a rapper/producer/artist?
I wanted to be all of those things the instant rap music discovered me. I learned that rap and I were meant for each other at around age 15.
3. How did you get started?
I became enamored with rap music as a mere child. I started making my own in secrecy as a young kid because it was unlike anything I had ever heard and I loved that dearly.
I actually tried to DJ before I took rapping seriously. Turns out I wasn't very good at it.
I continued rapping as a teenager when other people heard it and reacted positively. When school disinterested me, or when I was in trouble, I was armed and prepared with the necessary tools of a notebook, a pencil and headphones. I grew with it because when I grew as a person, people thought it sounded good or maybe even related. I utilized it as an expressive outlet as a young adult when I started to change; to explain my transformation to those I thought wouldn't understand without it. Turns out I was pretty good at it after all.
It's not the only thing I've ever been good at. But it's what I've been passionate about more than anything else.
It's been my channel throughout. Through adolescence. Through puberty. Through struggling with my identity. Through very real heartbreak. It's been with me longer than any friend or romantic interest. It's not disposable. It isn't sentient to abandon me and I could never even consider turning my back on it. Now I rap just so I don't lose my fucking mind. I think my girlfriend even gets jealous with how much I talk about it.
And I should've written this as a song.
4. Tell us a bit about yourself and your creative processes. What does your favorite environment to begin writing new lyrics or working on a new track look like?
Timothy Leary was a hack but his recommendation of a comfortable, familiar set & setting is reliable advice.
5. When does a new song feel finished to you?
Revision constantly yanks the reins. Some songs that I made publicly available five years ago I'll hear now and say "I should've said this" in lieu of the actual lyric. Once I decide the song can't be further sculpted to perfection in that moment, I put a helmet on it and shoot it into space.
6. What helps inspire your music?
Other music. Foreign music. Drama movies with hilarious one liners. Human interaction. Women. Staring at the moon and getting lost inside of myself. Challenging others to laugh or think, Having rappers for friends. That unspoken but acknowledged test to outdo each other while keeping it a hundred.
7. What advice has really stuck with you and what was the source?
Hours deep into a swamp a local rapper I looked up to chimed in on a conversation amongst our group about Wu Wear and his distaste for buying or wearing it.
Verbatim: "I'm trying to be a neighbor; not a fan."
That's always felt very powerful to me. I don't even necessarily think it was advice. More of a credo. It was either that or "Just drink some water and go to bed."
8. Fill us in about your latest project.
Its called "Brain Cave Deluxe". It's a throbbing, engorged hard on full of super unleaded gasoline. It's the cumulative sum of everything I mentioned already. The framed portrait of living inside my mind splattered over a choice backdrop of savory sounds.
9. What are the things about being a rapper that you most enjoy?
I'd say the women and free drinks but that'd be a bald faced lie. I'm handsome and drink a lot anyway so those things are just perks of being alive. The coolest thing about being a rapper is being able to get away with wearing a ton of purple without my boss asking uncomfortable questions.
10. What are you listening to right now?
Literally right now at 3:06 AM I am listening to Ege Bamyasi by CAN.
11. Who are your top 5 artists, either visually or musically these days?
This may fluctuate if you ask me tomorrow or any other day.
Thom Yorke
Omar Rodriguez Lopez
David Lynch
Andre 3000
Alejandro Jordorowsky
The kid who makes sidewalk chalk murals down the street. They're melty as f*ck when I'm walking to work.
12. If you can pick any artist or photographer to put together your album art, who would it be and why?
I want a moving holographic album cover directed by guy Ritchie featuring iridescent mannequins and gargantuan jellyfish.
The record was mixed and mastered by PJ Katz. Check out the production credits below.
- Meet Me In The Swamp – produced by Hunchback Esquire
- Cellar Door – produced by Hunchback Esquire
- Pork Swordsmen – produced by PJ Katz
- Up & Atom – produced by C-Note
- Pocketwatch Therapy – produced by PJ Katz
- Hi-Def Dream Theatre – produced by Hunchback Esquire
- Legit's Tooth – produced by Rob Viktum
- hippykoalafax // trapped in the hour of 8 – produced by Surge Cess
- Cities of Rust – produced by Radiolab
- Being Chased by Celestial Objects – produced by Theorist
- Infinity Gauntlet – produced by r0b0tr0n
- Elements – produced by C-Note
- With Hazel – produced by Melvin Junko
- War Pig – produced by elsphinx
- Got The Time – produced by PJ Katz
- Farewell, Flora – produced by Hunchback Esquire
- Moth – produced by Misnomer
- Pork Swordsmen remix – produced by J57


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"Efforts for compromise are being made while the offensive campaign is gathering steam and more newspapers are publishing the cartoon. There will be no compromise before we get an apology."
Related quotes
"It's amazing that the amount of news that happens in the world every day always just exactly fits the newspaper."
"I read about eight newspapers in a day. When I'm in a town with only one newspaper, I read it eight times."
"Indiana University Kokomo Chancellor Michael Harris' dedication to scholarly work and his successful leadership in higher education have earned him recognition by one of Israel's leading newspapers [Maariv] as the top Israeli in academic worldwide working outside Israel."
"In our newspaper segment we reported advertising gains at our U.S. community newspapers, particularly in classified employment and real estate while auto remained soft."
"Don't worry over what the newspapers say. I don't. Why should anyone else? I told the truth to the newspaper correspondents - but when you tell the truth to them they are at sea."


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School Routine
The school week runs from Sunday to Thursday inclusive and the timings for all primary children are 07:45 until 14:30.
After school clubs, for those taking, part run until 15:15.
Late Arrival
School recognizes that good punctuality and attendance are important life skills and as part of our new Punctuality and Attendance Policy, we are aiming for 90%+ attendance and 95%+ punctuality. Arriving late at school is unsettling for all children and staff. Please ensure that your child arrives in plenty of time. Children arriving after registration will be marked as late. Registration (taking attendance) is a crucial time of day where outline instructions are given for the rest of the day. Your support in getting your child to school on time is appreciated..
End of Day Collection
Children should be collected in person at the end of the school day (14:30). They will not be allowed to go to the gate or car park alone and must be collected by the parent/ guardian or named individual. Each class teacher will explain where children from that class must be collected. If you know that you are going to be late, please let us know at school so that we can tell your child and him/she doesn't get anxious. Please note, supervising children that have not been collected means that staff are taken away from their work of school improvement, preparation, planning and assessment. At the end of the day, both school gates will be open to ease congestion. Should you need to collect your child within the school day, please inform the class teacher in advance when possible. Please come to Primary Administration in order to collect a permission slip before going to collect your child.


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I remember my morning commutes before kids and hunty they were the worst: crowded trains, foul stench and blank faces that winced when you sat down beside them. Now that I'm a carpooling mama, my commutes are so much better: less crowded, 'cept on those days when everyone crams into the 2nd row because no one wants to ride in the 3rd row, occasional farts and "oops, I forgot to put on deodorant" and smiling faces - yep, that pretty much sums up my commute.
Our morning commute is just about 15-20 minutes but it's quality time we have. I always say "Have a great day" and "Be nice to your teachers" as each kids exits the vehicle. Then one day my middle kid hit me with "You have a better one." Made my day! Just out of nowhere, "You have a better one." Melted my heart.
Our evening commute gives us a few minutes to talk about how our day went. I ask each kid how their day was. I get the scoop on grades and the happenings of their day. Funny how the ups and downs of my work day fade away as I listen to my kids talk about their day. Occasionally, one of them will ask me about my day but it's so good to hear about the ups and downs of their day.
Being a parent has taught me so much and it's moments like our commute in which I really see just how much they've grown. Our time together has also taught me a few things. Trust me my commute is so much better with kids than before.
1. Patience
Before kids I confess I would honk, yell, shake fist and cut off drivers who pissed me off. Now that I'm a mom, I occasionally honk my horn to show disgust at a fellow driver but the yelling at drivers who cannot hear me, shaking of fists and cutting off others has ceased. Yeah, I get a little upset when another car cuts me off while my kids are in the car but I am a little more reserved in how I deal with it. Well, 'cept for that one time when a chick didn't yield and almost hit my car. I forgot one of the kids was in the car and yelled out my window "Hey lady, driving instructions" and then mumbled a few not so friendly things before I heard a whisper of "Mommy?". Oops! I totally forgot my kid was in the car.
2. Morning radio is NOT for kids
Another HUGE thing I've learned during my commute with kids is most radio stations are not for kids. I gave up on hopes of morning radio offering kid-friendly music shortly after Disney radio ceased in our area. I tried a few stations but it never failed a song would air resulting in one of the kids asking "Mommy, what's this song about?" or worse yet me praying the version being played is the "clean version". Then there's hearing your kid sing along to a song leaving you wondering where in the world they heard the song from.
Have you listened to morning radio lately? OMG.?! From the recaps of The Bachelor and the Real Housewives, it's a constant reminder the programming is not for kids. We tried listening to classical music in the morning but too mellow for the morning. We need something upbeat. Occasionally, we will tune into satellite radio but the advertisement to song ratio is 10:1. What's a mom to do? Christian radio offers some respite thanks to artist like Hillsong. The kids are really digging Hillsong Young and Free's latest CD release, . Then when they saw a video of Selena Gomez performing with Hillsong Young and Free, the track, "Nobody", has been a frequent song of choice during our morning commute.
3. I've Got a "Mom Arm"
Lastly, my morning commute has taught me I have a "Mom Arm". It takes a few years to develop the "Mom Arm". It's a rite of passage for kids from the car seat to the front seat. You know the "Mom Arm"? The reflex of extending your right arm across your child's chests whenever they sit in the front seat. I remember the first time my daughter rode in the front seat. I was a wreck. She was so excited. Moi? I was a nervous wreck. Needless to say, my rides with kids in the front passenger seat are few. I'm that mom you pull up beside, look over and see what appears to be a crazed woman talking to herself when in fact I'm talking to kids in the 2nd and 3rd row of my SUV. Yep, that's me. *points to self*
What's your morning commute like? Do you carpool with the kids? Do you take the train? I'd love to share the latest release from Hillsong United Young and Free with you to brighten up your morning commute.
Win It: One lucky Three Boys and an Old Lady blog reader will win a
Hillsong United Young and Free "Youth Revival" CD of their very own.
Hillsong United Young and Free "Youth Revival" CD of their very own.


text: movement sensor Adjust content blocking thearmydude next random Comment Top NextComment Thanks my fellow thumbers, I jizz my pants every time i get a good reaction. anyways check out this youtube link I posted, I had this on repeat for like 40 times in a row and couldnt stop laughing. Share some thoughts/thumbs on this content please-------> /youtube/2068481/hebrew+crunk/ next Tags: no | jews | were | harmed | on | the | description | link | well | Maybe | a | little T SAID DO T LOOK FAT ... +2134 Views: 44261 Submitted: 05/03/2011 Facebook Twitter Google+ Tumblr Pinterest


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Common Type
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Cautious Mother Crow
- #0640
- Eastern European (culture)
- Flying (attribute)
- Friendly (behaviour)
A crow from the old tales of Eastern Europe usually told to children. Cautious Mother Crow took great care of her young. When they hatched from their eggs, Cautious Mother Crow taught them to be careful of all the dangers in the world. She once taught them that they should be careful around people if they saw him or her picking up a stone. If they saw this they should instantly fly away. One of her children asked what they should do when they see a person who already has a stone in their hand. Cautious Mother Crow then knew that her children were wise and careful enough to leave the nest.
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© Copyright 2013 Mythical Creatures List Cautious Mother Crow
Background Illustrations (Left top-bottom, right top-bottom): Medusa by Gonzalo Ordonez, Loch Ness Monster by dyb,
Basilisk by JustMick, Shuck by Serphire, Ts Um A Kas - Illustration of a rock painting (from Dover publications).


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No real movement on the depth chart takes place during the first week of practices leading up to the first scrimmage. Now that the first scrimmage is out of the way, some slight changes have been made as the two-deep roster begins to take shape.
Shakeup at Inside Linebacker
Head Coach Bronco Mendenhall laid out for the media his current depth chart at inside linebacker, stating that if the season were to start today, Matt Bauman and Matt Ah You would be his top guys in the middle.
"From this point I think Matty [Ah You] has a slight edge on Shawn Doman," said Mendenhall. "But it's slight and you could basically list them as even. But if we had went today it would have been Bauman and Ah You, and then with Iona Pritchard and Shawn Doman being with the 2s and with the fifth player being Spencer Hadley."
Although Ah You has seen most of his reps with the 1s at Mike linebacker, he'll be moved over to Buck when Bauman comes back to fully participate. Bauman is on track to return later this week to full-contact drills according to Mendenhall.
Wagner on the Rise
Monday's morning practice also saw Jadon Wagner playing in place of Michael Alisa with the second-team defense. After reviewing the film coaches saw Wagner's physical presence as being a positive for the defense, earning him more looks as the possible backup to Vic So'oto.
"He's playing over the tight end," said Mendenhall about Wagner. "He's played very physical and I thought he had a good day in a run-oriented scrimmage, and this sort of scrimmage matched what I think his skill set is."
Mendenhall mentioned that they're still encouraged with Michael Alisa's progress, but that he needs to keep up with what the team is doing more if he's to earn a spot on the two-deep roster.
Three Targets
Mendenhall indicated that after Saturday's scrimmage there were three clear target areas that need to be addressed in earnest.
"After Fui [Vakapuna] and Harvey [Unga], the backup running back positions need to perform at a higher level," saidd Mendenhall regarding the first target.
Most of the concern with the backup running backs probably has to do with their ability at picking up blitzes and blocking effectively rather than in running the football.
The other areas Mendenhall stated as an area of concern were with the cornerbacks and outside linebacker.
"After watching the film I didn't think our cornerbacks played particularly well," said Mendenhall. "And then [the final issue is] just continuing to establish depth at outside linebacker."
Other than those three areas, Mendenhall stated that he was generally happy with the overall execution on both sides of the ball, and that they accomplished what they set out to do during the scrimmage.
Rich Settling In
Slowly but surely safety Andrew Rich has worked his way into becoming the third safety behind Kellen Fowler and David Tafuna. Establishing such a spot at safety is no easy task, as the free safety position is regarded as one of the two toughest positions on defense to learn and to play.
"Free safety and Mike," answered Mendenhall when asked of the toughest position to play in BYU's defensive system. "Those two positions are similar in that they control what everyone else does. The free safety spot is more visible, so if [someone] makes a mistake, then I'd say that increases the level of difficulty."
Rich has been meeting the challenge of playing free safety, as he has made significant progress this offseason and thus has earned some more trust from his defensive coaches.
"I've worked hard and I've tried to apply everything I've been taught since spring with every day," said Rich. "It's becoming easier with every day I'm out there. There are a lot of reads you have to make and it's not an easy thing, but it's definitely coming."
The hump most players have to get over at such a position as free safety is not only learning where to be as dictated by what the offense presents, but being so adept at recognizing a particular read that they don't think have to think about where to go and can instead just go there instinctively, thereby playing up to speed without any hesitation.
"That's the trick," said Rich about reaching the point where he can play full-out. "In spring there was a lot of thinking and then reacting, and now I feel that I'm just reacting as where I need to go and what my assignment is [are] coming more instinctively, which allows me to play faster."
Rich has caught the eye of his coaches and is recognized as a player who is having a great fall camp session one week in.
"He's been one of the bright spots of camp," said Mendenhall of Rich. "He's our third safety at this point and I feel comfortable in playing him."
Rich's story about refusing scholarship offers from such schools as Cal and Boise State to walk on to BYU has been well documented. Rich chose BYU primarily because of its environment, and on Monday Mendenhall stated a desire to reward Rich accordingly due to his desire to be part of the program and due to the strides he's made out on the practice field.
Rich said he's pleased with the progress he's been able to make, and gave credit for that progress to his teammates.
"I watch and learn from Kellen Fowler a lot, obviously," said Rich. "He's so smart with what he does and he continues to coach me as much as anyone when I'm out there on the field, which I certainly appreciate. We have a very talented defensive backfield. I know there is a lot of concern about our inexperience, but we have some great athletes at each position and we're going to surprise people with how good we are this year."
Practice Notes
-Recruits Richard Wilson and Remington Peck visited practice on Monday.
-Strong safety David Tafuna did not dress due to having his bell rung during Saturday's scrimmage. Tafuna will be further evaluated, and at that point it will be determined when he'll be able to practice again.
-Jordan Pendleton took Tafuna's place with the 1s on defense and Shiloah Te'o moving up with the second team in Pendleton's place.
-Matt Putnam saw all his reps with the second-team defense as he works to earn a spot in the defensive rotation.
-Mosese Foketi saw reps with the second-team defense and recorded a sack during 11-on-11s.
-Most of Monday's practice was focused on blue zone execution, with the highlight being G Pittman knocking away a pass intended for O'Neill Chambers in the back of the end zone.


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Silver [CD]
- Artist: Iona
- Release Date: 09/30/2011
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- Les Mardi Gras/Reuben's Train/Le Reel Béatrice: Medley
- Willie's Auld Trews/Fionnghuala/Tam Lin: Medley
- Morwnad yr Ehedydd/Titrwm Tatrwm/Abergenni
- Volviendo al Valle/Chouteira/Get You a Copper Kettle: Medley
- Tail Toddle/The Morning Dew/The Ale Is Dear: Medley
- Seán Bháin/Chloë's Passion
- Les Poules Huppées/Ton Bal Eured/Tumbledown: Medley
- Pachipi/Dans les Prisons de Nantes/Johnny's Gone to France: Medley
- The Trooper and the Fair Maid/Toddy MacPherson's
- Moirney ny Cainle/Arrane y Guilley Hesheree/St. Ives Fer Moh: Medley
- Shetland Reels: Spootiskerry/Sleep Soond i' da Moarnin'/Faroe Rum/Willafjord: Medley


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Attorney General Jeff Sessions Jeff Sessions Ann Coulter vows to speak at Berkeley Dem rep: 'Dreamer' shouldn't have been deported Freddie Gray's death must continue to be a catalyst for reforms MORE says the MS-13 gang could be designated as a terrorist organization.
Asked by Fox News's Tucker Carlson on Tuesday whether he thinks the designation would be helpful, Sessions replied, "I think so, perhaps. I believe it could qualify for that."
"There are rules that I guess the State Department does to establish that," he added. El Salvador has already designated MS-13 a terrorist group.
Sessions called the gang "one of the most violent gangs in the history of our country."
"We can devastate this gang. We're going after them," he said. "We're not going to allow them to take over a block, a corner of our communities and terrorize people with this violence."
Speaking to a meeting of the Organized Crime Council on Tuesday, Sessions blamed open borders and lax immigration enforcement for the growth of the crime gang, which was founded in Los Angeles in the 1980s by Central American immigrants mostly from El Salvador.
He said his Justice Department would have "zero tolerance" for the gang violence.
"We cannot allow this to continue. We will secure our border, expand immigration enforcement and choke off supply lines," said Sessions.
Members of MS-13, or Mara Salvatrucha, are known for their violent methods and fierce gang loyalty. According to the National Gang Intelligence Center, MS-13 has more than 10,000 members in the United States, and more than 30,000 worldwide.


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Go Green
I have been working towards creating a paperless office over the last few years and I have finally implemented the last stage of this process. So to celebrate this milestone and all things green I am sharing with you this cool new fresh mint colour that I adore. May it inspire us all to do more in an effort to "Go Green".


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Must Watch: Noynoy Aquino Nag-deny Di Daw Sya Kasama Sa Mamasapano Operation!
We overheard that former President Aquino III has his counter affidavit. Aquino's counter affidavit contradicts the findings of Senate, PNP-BOI which found him responsible for Mamasapano mission.
Aquino said "I could have immediately ordered and directed the conduct of reinforcement measures". Aquino also said he was fed false and misleading information.
He also said "I did not directly participate in the planning and execution of Oplan Exodus, neither did I approve it".
Napenas said to former President Aquino" Stop Blaming Subordinates, Face the Truth."
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Tanner Foust took the Red Bull Global Rallycross points lead in emphatic fashion with a dominant performance in the final of the second part of the Ottawa double header event.
Monday, 19 June 2017
Steve Arpin triumphs on home soil
Steve Arpin took an emotional home victory in Red Bull Global Rallycross' first ever visit to Canada, taking the points lead in the process.
Thursday, 15 June 2017
Andretti explains Speed e.dams snub
Andretti Autosport have explained the reasoning behind the decision not to let Scott Speed race for e.dams Renault in next month's Formula E double header in New York.
Red Bull GRC champion Speed's Formula E return thwarted
Renault e.dams hopes of drafting in Scott Speed to replace Sebastien Buemi for next month's New York double header have been dealt a blow.
Tuesday, 13 June 2017
Red Bull GRC Fantasy Challenge Picks – Ottawa
Red Bull Global Rallycross makes its first visit to Canada this weekend, which will also be the championship's first rounds outside of the US since 2015's double header event in Barbados.
Monday, 5 June 2017
Mikko Hirvonen rules out immediate WRX future
Mikko Hirvonen has said that he won't be making a move into rallycross "in the near future", despite recently testing the JRM Mini Countryman RX at Silverstone.
JRM boss coy on Red Bull GRC rumours
JRM Group managing director Jason King has downplayed talk of his company's Mini Countryman rallycross car competing in Red Bull Global Rallycross.
Arpin ends Foust's run in New England
Steve Arpin took a surprise win in the second part of the Red Bull Global Rallycross double header in Thompson, Connecticut after Tanner Foust retired from the final.
Two poles from two for Tanner Foust
Tanner Foust continued his dominant performance at Red Bull Global Rallycross in Thompson, Connecticut by claiming his second pole position in as many days, and his third in as many events.
Saturday, 3 June 2017
Foust completes flawless performance in New England
Tanner Foust completed a perfect event by dominating the final of the first part of the Red Bull Global Rallycross Thompson double header.
Tanner Foust takes pole in Thompson
Tanner Foust has taken pole position for the first event of the Thompson Red Bull Global Rallycross double header.
Red Bull GRC Fantasy Challenge Picks – Thompson
Red Bull Global Rallycross returns to New England this weekend for its first visit to the area since 2013
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Daily Rentals
Fleet Services has a fleet of sedans, minivans, pickups or work vans available for daily rental needs. We have a new online system to request vehicles for your short term U-M business needs. The online system requires your uniqname and Kerberos password to access the system. You must be an approved driver or submit a request within the system for system access. View the instructions to request access (PDF).
Vehicle requests require approved drivers in order to reserve a vehicle. To seek approval to drive on behalf of the U-M, you must complete the online motor vehicle record check.
To reserve a vehicle for daily use, please use our daily rental reservation system. If you have any questions or need assistance, please follow these instructions (PDF) or contact us by email at email@example.com or by phone at 734-764-2485.
Currrent daily vehicle lease rates are as follows:
|Vehicle Type
|Passenger Capacity
|Daily Rental Rate
|Compact Pick-up
|2 or 3
|Full Size Pick-up
|Minivan (accessible)
|5 - 2 Wheelchairs
|Sedan (hybrid)
|Sedan (non-hybrid)
|Work Van
Daily rental rate is based on a 24 hour clock with a minimum charge of one day - ie. Vehicle picked up at 8 am and returned by 8 am the following day is equal to one day charge.
A cancellation charge will be assessed when notification of the cancellation is less than 24 hours prior to the requested departure time. The charge is equal to a one day charge for the type of vehicle reserved.
A late return charge will be assessed when the vehicle is not returned by the requested return time. The charge is equal to two days charge for the type of vehicle reserved, for each day the vehicle is late.
The daily rental rate does not include fuel. When you pick up a rental vehicle, the vehicle is full and you are billed for re-filling the tank upon vehicle return.
Fleet Services normal business hours are Monday – Friday (except holidays) 6:30 am until 5:00 pm. All rental requests for use on the weekend (Saturday or Sunday) must be picked up on the preceeding Friday afternoon between 1:00 pm – 4:00 pm.
Vehicles may be rented by departments for use by U-M faculty and staff to conduct U-M business both on and off campus. The use of a vehicle and purpose of the trip is restricted to U-M business only.
Registered student organizations may rent vehicles as long as there is a U-M business need to do so. For sponsored student organizations, the rentals must be approved by a dean, director or faculty advisor prior to the rental request being forwarded to our office. For volunteer student organizations, the rental must be approved by the Center for Campus Involvement, who will forward the rental request to our office. These rentals must be pre-approved, and include such activities as travel to conferences, competitions, field trips, or other appropriate educational opportunities that are relevant to current curriculum or the U-M mission.


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Hull have denied reports Phil Brown has been sacked as their manager.
A number of bookmakers, including Paddy Power and William Hill, today suspended betting on Brown being the next Premier League manager to leave his job, while Dean Windass - the striker who fired Hull into the Barclays Premier League with the only goal in the play-off final victory in 2008 - has also called for the 50-year-old to depart the KC Stadium.
However, a statement tonight on the club's official website, www.hullcityafc.net, read: "Contrary to media speculation the club can confirm that Phil Brown has not been sacked and remains the manager of Hull City Football Club."
Windass called for Brown to "get moved on" amid the mounting speculation over the Hull manager's future.
Brown helped the club escape relegation in May but has eight points from 10 games in the current campaign, and they are only above Portsmouth and West Ham in the table.
Windass, who left Hull in January, told talkSPORT magazine: "I think sometimes you need a fresh change. Phil's done a fantastic job since he's been there but I think it's time that he should get moved on and bring somebody else in."
Windass, questioning whether the club can stay up this season, added: "It's going to be difficult. They're not getting results and they're struggling to score goals at the moment.
"They need a 10-15 goal-a-year striker to get away from the bottom of the table. They've got a little mini-league at the moment and it's important they win games against teams in and around their league."
Brown had earlier today given an interview to a local newspaper insisting he would not quit the club, although rumours still started spreading about his future.
There have also been concerns about the club's long-term financial position, stemming from accounts being published covering the year ending July 2008.
In that 12 months Hull made a loss of nearly £10million as they won promotion to the Premier League via Windass' goal at Wembley.
This does not include any figures from the club's first season in the top tier of English football, and the directors remain confident the next set of results will be much more positive.
- More about:
- Glamour Magazine
- Local Newspapers
- Newspapers And Magazines
- Phil Brown (Football Manager)


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CHAPEL HILL, N.C. – No. 10 North Carolina will play for its school-record 12th win of the season in the Russell Athletic Bowl against Baylor.
"I can tell you that the North Carolina Tar Heel football team is extremely excited to play in the Russell Athletic Bowl in Orlando," Fedora said during a teleconference call on Sunday. "And then to be able to play a great opponent like Baylor University and a team that Art Briles is coaching makes it even that more special."
Despite its ACC Championship Game loss to Clemson on Saturday, UNC remained in contention for a New Year's Six bowl game before falling one spot shy of inclusion in the final College Football Playoff Rankings.
Due to the Sugar Bowl not being one of the semifinal games this year, the Big 12 and SEC champions (Alabama and Oklahoma) left a contract void due to their participation in the College Football Playoff. As a result, No. 12 Ole Miss and No. 16 Oklahoma State jumped UNC in the pecking order.
No. 18 Houston secured a NY6 bowl as the Group of 5's highest-ranked champion. After those contractual obligations were met, the remaining teams ranked highest by the selection committee playoff filled the rest of the NY6 bowls. No. 9 Florida State was the final team to make the cut.
Being left out of the New Year's Six games, however, will not become a focal point for the Tar Heels.
"Our team is not going to be disappointed to be in the Russell Athletic Bowl in Orlando because it's a great place to be against a great opponent," Fedora said. "There will be no worry that we didn't get into a New Year's Six game. Our guys are excited to play. We're anxious to get the opportunity to play one more game."
While the Tar Heels (11-1, 8-0 ACC) and Bears (9-3, 6-3 Big 12) will play for the first time, UNC head coach Larry Fedora has a long history with the Baylor program. His first coaching job came at Baylor in 1991 as a TE/WR/RB coach under Grant Teaff, and two of his daughters were born in Waco.
"You could say it's where I cut my teeth on college football," Fedora said.
UNC is making its second trip to Russell Athletic Bowl, albeit under a different name and location. The Tar Heels defeated Arkansas, 20-10, in the 1995 Carquest Bowl in Miami Gardens.
This will be UNC's seventh bowl appearance in eight years.


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Oh, songs of the little birds!
Do bring comfort to my heart-broken self.
You are a bunch of beautiful creations,
That can swept away my uneasiness.
But the power of God that creates,
It is impossible for me to possess.
Who am I to yearn for that?
Greediness and arrogance will somehow lead me to death.