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Ensembl variation resources
The maturing field of genomics is rapidly increasing the number of sequenced genomes and producing more information from those previously sequenced. Much of this additional information is variation data derived from sampling multiple individuals of a given species with the goal of discovering new variants and characterising the population frequencies of the variants that are already known. These data have immense value for many studies, including those designed to understand evolution and connect genotype to phenotype. Maximising the utility of the data requires that it be stored in an accessible manner that facilitates the integration of variation data with other genome resources such as gene annotation and comparative genomics.
The Ensembl project provides comprehensive and integrated variation resources for a wide variety of chordate genomes. This paper provides a detailed description of the sources of data and the methods for creating the Ensembl variation databases. It also explores the utility of the information by explaining the range of query options available, from using interactive web displays, to online data mining tools and connecting directly to the data servers programmatically. It gives a good overview of the variation resources and future plans for expanding the variation data within Ensembl.
Variation data is an important key to understanding the functional and phenotypic differences between individuals. The development of new sequencing and genotyping technologies is greatly increasing the amount of variation data known for almost all genomes. The Ensembl variation resources are integrated into the Ensembl genome browser and provide a comprehensive way to access this data in the context of a widely used genome bioinformatics system. All Ensembl data is freely available at
The amount of publicly available biological sequence data has been increasing exponentially over the last decade. In addition to the many reference genome sequences now available, variation data is being produced in significant quantities. These data fundamentally seek to extend our knowledge of the genome sequence from the concept of a single "reference" genome sequence, representing a single individual, to a more comprehensive understanding of the genomic diversity of entire species.
Today most variation data is produced in the context of large-scale genotyping assays or resequencing projects which focus either on the whole genome or selected functional regions of the genome such as protein coding regions, regulatory regions or sites of known disease mutations. One of the larger resources includes a comprehensive haplotype map of the human genome created by the International HapMap Project, based on DNA from 270 individuals from four populations
These huge datasets including variation data are often available from the original sources in a variety of formats requiring the development of various methods to integrate, archive and display these data in a consistent fashion. Ensembl
The Ensembl project is a comprehensive bioinformatics resource for chordate genomes. Thousands of researchers from around the world access Ensembl data every day through the various portals provided by the project including the web interface at
Ensembl is updated approximately every two months with newly sequenced genomes and newly available or processed data for existing genomes. The project specialises in integrating large-scale data from many different sources in a variety of formats with a high-quality annotation of the genome and gene set. In addition to comparative and functional genomics data resources, Ensembl provides variation data for a number of supported species.
Each year, Ensembl publishes a general update of all the project's resources
Construction and Content
Ensembl produces variation databases for a subset of the genomes available in Ensembl (first two columns of Table
Table 1
Variation statistics for release 56, September 2009
Number of variants **
Genome size* (Assembly name)
Data source of variants
Homo sapiens
3,101,804,741 (GRCh37)
dbSNP; Resequencing reads
Pan troglodytes
Pongo pygmaeus abelii
Assembly Reads
Mus musculus
dbSNP; Resequencing reads
Rattus norvegicus
dbSNP; Resequencing reads
Canis familiaris
Bos taurus
2,918,205,644 (Btau_4.0)
Ornithorhynchus anatinus
Assembly reads
Gallus gallus
Danio rerio
1,481,257,891 (Zv8)
Tetraodon nigroviridis
Assembly reads
Taeniopygia guttata
1,233,169,488 (taeGut3.2.4)
Assembly reads
*Genome size refers to the length of the reference assembly.
**Distinct mapped variants
The Ensembl variation resources are updated when a new genome assembly is released, a new set of gene annotations is available or revised, an external data source such as dbSNP at the NCBI is updated or a major new data collection becomes available.
Variation data is stored in a structured MySQL database. A companion paper describes the underlying database technology that is used to efficiently store and manage this data and the associated Application Program Interface (API) for programmatic access to the data
Data Sources
Importing variation data from dbSNP
The bulk of the SNP and in-del variation data in Ensembl is an imported dataset from dbSNP. This information is selected from dbSNP's largest tables. Table
Table 2
List of Ensembl tables generated and dbSNP tables used
SNP, SNPAncestralAllele
variation synonym*
SubSNP, SNPSubSNPLink, ObsVariation
Batch, UniVariAllele
population/sample/population structure/sample synonym
PopClassCode, PopClass, PopLine
individual/sample/individual population/sample synonym
SubmittedIndividual, Individual PedigreeIndividual
Allele, AlleleFreqBySsPop, SubSNP
flanking sequence
SubSNPSeq5, SubSNPSeq3, SNP
individual genotype
SubInd, ObsGenotype, SubmittedIndividual
population genotype
GtyFreqBySsPop, UniGty, Allele
variation feature
SNPContigLoc, ContigInfo
Normally, the reference genome assembly used by Ensembl matches the one used in the current build of dbSNP. When this is the case, the variant mapping positions can be imported directly from dbSNP and are therefore in sync with the rest of the data in Ensembl. As the genome browser at UCSC also uses the dbSNP variant mappings, using the same assembly is good for consistency across the major genome browsers.
In some cases however, the current genome assembly supported by Ensembl is ahead of the one used in the most recent build of dbSNP. When this occurs, the variants and their flanking sequences need to be remapped to the new assembly using a method in the core Ensembl API. For those variants that fail to map, new mappings are obtained using ssaha2, an improved version of SSAHA
The effect of this procedure is to ensure that dbSNP variation data in Ensembl is mapped to the most recent genome assembly supported by Ensembl, even if the assembly is not yet supported by dbSNP. As noted above, when the new dbSNP build is released, Ensembl is updated with the dbSNP defined variant positions. For a very small number of SNPs, this dbSNP update will result in changes to the SNP locations that had been defined by the Ensembl mappings on the new assembly. These changes are due to the method of incorporation of newly submitted data by reclustering of the variants as part of each dbSNP build.
Genotype data is imported from dbSNP and integrated with the polymorphism data described above. For example, in release 56 Ensembl has genotype data for 8335 individuals from a number of reference data sets including the HapMap
Array Data: Illumina, Affymetrix
Primary identifiers are assigned to variations. Usually these are from dbSNP (rs and ss identifiers). However, there may be other sources for variations. Names in these other datasets are only used as primary IDs if there is no matching dbSNP record. In the case of a dbSNP record, the IDs from other datasets are included as synonyms.
As of release 56 (September 2009), Ensembl imports variants positions from the following arrays: Affymetrix GeneChip Human Mapping 100 K Array Set, Affymetrix GeneChip Human Mapping 500 K Array Set, Affymetrix Genome-Wide Human SNP array 6.0, Illumina whole genome SNP genotyping chips designed for association studies (Human 660W-Quad, Human 1M-Duo V3) and for cytogenetic analysis (Cyto SNP-12 v1).
Legacy variation identifiers
Previously, Ensembl provided the identification page numbers from The SNP Consortium
Phenotype data
Ensembl currently imports phenotype data from two sources: The National Human Genome Research Institute's (NHGRI) Office of Population Genomics and from the European Genome-phenome Archive (EGA) database
NHGRI has developed a curated database of published significant genomic regions identified from genome-wide association studies
The EGA database at the European Bioinformatics Institute is designed to provide a permanent archive for all types of personally identifiable genetic data including genotypes, genome sequence, and associated phenotype data.
In Ensembl release 56 (September 2009), data from these two resources total 134 phenotypes from 1120 possible phenotype annotations associated with 142 different variants. There is a direct link from the web page to the original submitter's data entry under the "Source" column in order to acknowledge the contributor directly. In addition a link is provided to the publication record in PubMed.
Data generation
Algorithm for Variant discovery from Sequencing reads
Ensembl provides the location of specific variants in individuals, laboratory strains and breeds. These variants are calculated as described here using publicly available Sanger-style resequencing data such as from the Ensembl Trace Archive or from whole genome projects that sequence a single individual (with both haplotypes) or small number of individuals. By aligning the sequencing reads to the reference assemblies for the same species, Ensembl is able to identify any heterozygous sites in the sequenced individual by mismatches in the alignment
Furthermore, potential variants are filtered and discarded if they have more than 30 mismatches from a single sequence read or are in a region with more than 100 aligned reads. (These values are reduced if the sequencing coverage is less than approximately 0.1×). Read pair information is used to filter out wrongly mapped reads, and variants with more than two alleles for one strain/individual are discarded. For individuals or strains with a read coverage of greater than 3, a SNP will be reported if at least two reads have the minor allele.
Ensembl also examines the resequencing reads that align to known SNP loci in order to verify the sequence of the resequenced individual at these locations. If there is sufficient evidence (defined with the same quality metrics as above) to determine that the given individual does not have an alternative allele at the tested location, this information is stored in the database as a "SARA" (Same As Reference Assembly) position, which can be thought of as a computational genotyping assay.
A representation of the resequencing coverage for several mouse and rat strains as well as the Watson and Venter genome sequences is stored in the variation database as a range of start and end coordinates with an indication of the level of read coverage.
The variation database generated by Ensembl's SNP calling pipeline is then merged with the variation database imported from dbSNP. Part of the merging procedure involves modifying all of the dbSNP imported variants to represent them on the positive strand. A variant by Ensembl from resequencing data that has the same locus as one already in dbSNP is assigned the rsID from dbSNP for that locus as the primary ID; those not yet in dbSNP can be identified by the Ensembl style identifiers and are submitted by Ensembl to dbSNP.
Data quality checks
Once the database has been populated with variation data, a series of post processing steps is performed to ensure data consistency and quality. Variants observed multiple times (e.g. in data imported from dbSNP and created by analysis of the Ensembl Trace Archive) are collapsed into a single record with supporting identifiers stored as synonyms.
Variants are considered unreliable and flagged as "failed" if they exhibit any of the following characteristics: variants which map more than three times; those which do not map to the genome; those with no observed allele matching the reference allele; those with more than three observed alleles. Failed variants remain in the database but have no associated mapping position. There is also a checklist to ensure those medically important or other known to be correct variants that exhibit one of the above characteristics are not moved inadvertently to the failed variation table. Approximately 689660 variants are classified as failed across all supported species in release 56 (September 2009) (see Table
Table 3
Breakdown of failed variants by type for each species
Type 1
Type 2
Type 3
Type 4
Type 5
Total number
Homo sapiens
Pan troglodytes
Pongo pygmaeus abelii
Mus musculus
Rattus norvegicus
Canis familiaris
Bos taurus
Ornithorhynchus anatinus
Gallus gallus
Danio rerio
Tetraodon nigroviridis
Taeniopygia guttata
Type 1: Variant maps to more than 3 different locations
Type 2: None of the variant alleles match the reference allele
Type 3: Variant has more than 3 different alleles
Type 4: Loci with no observed variant alleles in dbSNP
Type 5: Variant does not map to the genome
Variation consequence annotation
Once a list of reliable variants has been identified, they are integrated with the Ensembl gene annotations in order to estimate the consequence of each variant on all transcripts. A list of possible consequences assigned in shown in Table
Table 4
List of consequence types in the transcript variation table
Consequence type
Sequence location
Stop gained
causes a gain of a stop codon
coding sequence of the peptide
Stop lost
causes a loss of a stop codon
coding sequence of the peptide
complex InDel
change in nucleic acid
Indel spanning exon/intron or CDS/UTR border
Frameshift coding
causes a frameshift of the reading frame
affects the coding sequence of the peptide
Stop gained, frameshift coding
multi-allelic variant that introduces a stop codon or causes a frameshift
affects the coding sequence
Non-synonymous coding
causes an amino acid change
coding sequence of the peptide
Stop gained, splice site
causes a gain of a stop codon
1-3 bps into an exon
Stop lost, splice site
causes a loss of a stop codon
1-3 bps into an exon
Frameshift coding, splice site
causes a frameshift
1-3 bps into an exon
stop gained, Frameshift coding, splice site
Multi-allelic variant that introduces a stop codon or causes a frameshift
1-3 bps into an exon
Non-Synonymous coding, splice site
causes an amino acid change
1-3 bps into an exon
Synonymous coding
change in nucleic acid but no change in amino acid
coding sequence of the peptide
Splice site, synonymous coding
change in nucleic acid but no change in amino acid
1-3 bps into an exon
Regulatory region
change in nucleic acid
regulatory region annotated by Ensembl
Within mature miRNA
change in nucleic acid
within mature miRNA
change in nucleic acid
In 5' UTR
Splice site, 5' UTR
change in nucleic acid
1-3 bps into a 5' UTR exon
change in nucleic acid
In 3' UTR
Splice site, 3' UTR
change in nucleic acid
1-3 bps into a 3' UTR exon
change in nucleic acid
In intron
Essential splice site, intronic
change in nucleic acid
in the first 2 or the last 2 base pairs of an intron
Splice site, intronic
change in nucleic acid
3-8 bps into an intron
change in nucleic acid
Within 5 kb upstream of the 5'-end of a transcript
change in nucleic acid
Within 5 kb downstream of the 3'-end of a transcript
Within non-coding gene
change in nucleic acid
Within non-coding gene
change in nucleic acid
More than 5 kb away from a transcript
Variants are associated with transcripts if they are located within transcript or intron sequences, within 5 KB of a transcript start or end, or if they are located within a regulatory region associated with a gene or transcript. Ensembl ranks these "consequence types" in estimated order of importance, including any effect of the variant on the sequence of the final protein product. For example ESSENTIAL SPLICE SITE is considered one of the most important consequence types as it would have great affect on the protein product.
Calculation of Linkage Disequilibrium (LD)
The method used to calculate LD (r2 and D') is described below. For each population with a sample size of at least 40, variants are ordered by their positions and pairwise LD (r2 values) are calculated within a 100 kb window size. If r2 values are less than 0.05 they are discarded and the rest are stored in a table. This table is only used for the calculation of tagged SNPs and is not present in the final database. The r2 and D' values used in Ensembl web interface are calculated separately on demand
Calculation of tagged SNPs
A list of tagged variants for haplotype analysis is produced for each of the HapMap and Perlegen populations and stored in the database. This is designed to choose common variants in each region of the genome (i.e. tag SNPs) from which the genotypes of surrounding SNPs in high LD can be derived. In order to store a minimal list, variants are selected per population and any other variants in high LD with this one are filtered out. To do this, variants that have genotypes are sorted by start position. For each variant, the MAF is calculated by population and then these are ordered by frequency with the highest MAF first. For each ordered variant, the pairwise r2 value for all variants within a 100 KB is extracted from the LD table described above. If the r2 value between the variations is greater than 0.99 (i.e. the two variants are in high LD), the associated variant with the lower MAF is removed from the list. This process removes variation data in high LD with other variants that have lower MAF. The remaining variations are called tagged SNPs and are stored in the database.
Utility and access of variation data
As with all Ensembl databases, the data is accessible in multiple ways: either programmatically via the Ensembl-style Application Program Interface (API)
Data Visualisation
Ensembl provides a number of specialised and highly interactive web displays focusing on four levels: a genomic location, a gene, a transcript and a variation. These four types of view support distinct pathways into the data (Table
Table 5
Variation Displays in the Ensembl website
Browser Object
Sub Panel
resequencing data
linkage data
variation table
variation image
population comparison
comparison image
population genetics
individual genotypes
phenotype data
phylogenetic context
The variation tab
Consider the variation rs2476601, which has been well-studied (see for example
The variation tab contains data reports for one specific variation. A menu of links to these reports is available at the left hand side of the variation tab Figure
Figure 1
Variation summary for rs2476601
Variation summary for rs2476601. Label 1 indicates the links to information for this specific variant. Label 2 indicates the summary, which includes the source (dbSNP), synonyms or other IDs for this variant in other databases or array platforms, alleles found and genomic location. Flanking sequence is also found in the
LD plots
Following the link to an LD plot displays the LD data calculated within a region (Figure
Figure 2
LD (linkage disequilibrium) plot for the region around rs2476601
LD (linkage disequilibrium) plot for the region around rs2476601. LD values in this figure were calculated based on allele frequencies in the CEPH human population. The LD data between variants is represented using a triangular grid shaded on a gradient from white to red depending on the strength of the LD (where red is high LD, white is low). Hovering the mouse cursor over one of the coloured regions in the plot reveals a pop-up box displaying the two variation IDs for that coloured region, and the LD value between them.
Variation links
Links at the left of the page (Figure
Figure 3
Variation views
Variation views. Views available in the variation tab are titled with the name of the link leading to each set of information. The data in the figure correspond to rs2476601. The
Gene/Transcript link
A table displays any transcript in Ensembl associated with the variant. The predicted effect of the variant is listed (e.g. intronic, synonymous coding, etc.) along with the position of the allele in the spliced transcript and protein. These values can be exported using BioMart.
Population genetics
Data from projects such as The HapMap Project or Perlegen are included in this view. Allele frequencies are displayed for specific populations, along with a summary of genotype information.
Individual genotypes
This next view shows a breakdown of genotypes for each individual stored in the database.
The variant of interest is shown by default in a 30 Kb region with neighbouring SNPs as boxes and indels as triangles. Variation colour-coding is consistent throughout the Ensembl website and depend on the variant's localisation within a transcript (table
Phenotype data
Listed in tabular format are any associated diseases, appropriate study and PubMed references, associated genes, the highest-risk allele and associated variant.
Phylogenetic context
This view displays a section from a whole-genome, multi-species alignment in this region. These alignments, including the calculated ancestral sequences are produced by Ensembl's three step Enredo-Pecan-Ortheus (EPO) pipeline
The gene tab
Genetic variation: table and image
Clicking on an Ensembl gene identifier or searching for a gene in the Ensembl search box (e.g. ENSG00000134242) moves the display to the gene tab. The gene tab provides links to the genetic
Figure 4
Variation image
Variation image. A. The variation image in the gene tab shows all transcripts for an Ensembl gene in red, and variations mapping to each transcript as coloured boxes. Intronic variants are, by default, only drawn if within 100 bp of an exon. Change this by clicking on
In the
Gene sequence
The genomic sequence can be configured to highlight variant positions. Exons are highlighted in this view, allowing immediate visualisation of where a variation falls, in the context of a gene.
The transcript tab
Transcript variation: table and image
The gene-based variation pages described in the section above compare and collate information across alternative transcripts for the reference sequence assembly. The transcript views provide an in depth focus on one transcript, or splice isoform, across individuals, breed or strains. A list of these views and links are shown at the left of figure
Figure 5
Population comparison image
Population comparison image. The ENST00000359785 transcript is shown, along with a representation of the genomes of Craig Venter and James Watson and the reference sequence (GRCh37) in this region. The left hand side of the view shows links available from the transcript tab. Label 1: The genomes of Craig Venter and James Watson have high coverage in this region (i.e. at least two sequencing reads or present for both strands of the chromosome), shown by a thick grey bar. Label 2: Variants are drawn as boxes if the allele differs from the reference sequence. A non-synonymous variation is shown in yellow, with two potential amino acids indicated (tryptophan and arginine). Label 3: The nucleotides possible for this variation are displayed upon clicking the corresponding yellow box. Label 4: A table compares each allele at positions of variation. This view indicates the alleles in both Venter and Watson's genomes are different to the reference sequence allele, for this variation. Watson is heterozygous at this position, and Venter is homozygous.
The transcript tab may be reached by clicking on any Ensembl transcript identifier link within the browser. All transcript identifiers (such as ENST00000359785) have the letter T immediately before the series of numbers. In the
cDNA sequence
Variation data may be displayed on the spliced transcript sequence as in Figure
Figure 6
The cDNA sequence for ENST00000359785
The cDNA sequence for ENST00000359785. A. The untranslated region (UTR) is highlighted in yellow. Numbering of the first line begins at 1 at the start of the UTR. Numbering of the second line starts at 1 at the beginning of the coding sequence. The third line shows line numbering and sequence of the protein. Codons are indicated by light yellow alternating with no highlight. B. Further down the sequence shown in A, a variation is highlighted. This variation is at position 183 in the protein sequence, 547 in the coding sequence, and 677 in the transcript sequence is shown to be rs34590413. The ID is revealed upon clicking on the IUPAC or ambiguity code above the highlighted nucleotide, which provides a link to the variation tab. The non-synonymous variations are shown by a red-coloured amino acid. Hovering over the indicated amino acid with the mouse shows the possible amino acids at that position.
In figure
The location tab
The fourth and final tab described in this section contains location-based views, showing a region of the genome. View this tab by selecting it, or by clicking on any genomic coordinates-range presented in the browser. This tab was already visited in the data visualisation section of this paper as it houses the LD plots.
Variation data may be displayed in the
Figure 7
Sequence alignments between the human reference sequence, GRCh37, and the genomes of Venter and Watson
Sequence alignments between the human reference sequence, GRCh37, and the genomes of Venter and Watson. Variation data are highlighted in green and indicated by an IUPAC code. A. The default display replaces nucleotides with dots if the genome has the same allele as the reference assembly. B. The display can be changed by using the
Data export with BioMart
The BioMart tool utilises denormalised Ensembl databases and can be used to query the Ensembl core or variation databases
Starting a BioMart query and choosing the Ensembl database (for release 56, this is listed as "Ensembl 56" from the dropdown menu, where 56 refers to the September 2009 version of Ensembl) allows gene-specific information to be exported. For example all variation for a gene can be exported in tabular format. In addition, a user may input a list of gene IDs, a region, or even an entire chromosome to mine genes and associated variations.
The Ensembl variation database may also be selected as the starting point for a BioMart query. This allows input of variation IDs, and is not contingent upon a gene annotation (i.e. intergenic regions are allowed). Furthermore, variations can be compared across populations, strains or breeds, starting with the variation database and using the "strain polymorphisms" attributes page. Export includes tabular formats such as csv, tsv, and Microsoft Excel. Alternatively, FASTA sequences can be exported.
These BioMart queries can also be run programatically using a Perl API or through Web Services. Although the programmatic scripts can be written from scratch, the simplest way to access these interfaces is to build the variation query using the BioMart website and then use the "Perl" or "XML" buttons in the toolbar to produce the appropriate API or Web Services queries.
Additional information about BioMart queries is available from the BioMart help pages at
Data access using the API
Advanced data mining is possible through direct MySQL queries of the Ensembl public database server at ensembldb.ensembl.org or by using the Ensembl variation API
Data downloads
For convenience, Ensembl data is also available in species-specific files on the FTP site
Future developments
New data is expected to greatly increase the already vast variation information in Ensembl databases. The 1000 Genomes Project is generating a large number of individual reads and variation data that will be integrated into Ensembl. There is also additional reference copy number and structural variation (CNV/SV) data planned to be incorporated from the Database of Genomic Variants
The publication of many new genome wide association studies are expanding the list of alleles that can be reliably linked to disease phenotypes and both associated and causative variants will be incorporated into Ensembl as they are identified. Additionally, as part of the LRG project
Other future developments include more interactive analysis of user's variation data through the Ensembl web site including an online-based upload tool for which will enable users to upload their own set of variant positions and for these to be viewable on the genome browser and for Ensembl to estimate their effects based on their relative positions to the Ensembl gene sets.
Ensembl's variation resources provide a comprehensive and integrated resource for biologists and bioinformaticians to support a wide variety of research applications.
Continuously increasing biological information sources require effective and integrated bioinformatics tools and visualisation that relatively few sources are able to provide.
Ensembl's variation resources provide several advantages for users. The resources are fully integrated within to the Ensembl genome browser and with the high-quality Ensembl gene sets in order to estimate the consequences of each variant. The data is updated with each Ensembl release including automatic mapping when new genome assemblies are released, such that Ensembl's variation data may be provided in advance of a new dbSNP build. Given the breadth of species available within Ensembl, it is also easy to compare the information across many vertebrate and model organism species. Another strength of Ensembl is the availability of all its resources for free and in a variety of ways to suit a range of users as described in the availability section below. In particular, the variation API is unique among other large-scale integrative bioinformatics projects.
Finally, Ensembl is a service-focused resource. Comprehensive instructions and additional details about the project can be found at
All Ensembl data and source code are freely available for any purpose and may be downloaded in their entirety from
Authors' contributions
The database resources were created by YC, WM, DR, and FC. The variation-specific web displays were created by FC, EK, JS, BP and SB with input from all of the other authors. PMG and DS developed specific software for Ensembl variation. GS created the tutorials. FC, PF and EB defined requirements and PF provided overall project supervision. The paper was written by FC, PF, YC, GS and DS with input from all of the other authors. All authors read and approved the final manuscript.
We thank all of the Ensembl users, especially those who have taken the time to contact us with suggestions and other helpful comments.
The Ensembl project is funded primarily by the Wellcome Trust. Additional funding for Ensembl's variation resources comes from EMBL, the European Union Framework 6 STAR project, European Union Framework 7 Programme GEN2PHEN project and the UK Medical Research Council. We thank the other members of the greater Ensembl group at the EBI and the WTSI and Guy Coates, Pete Clapham and Tim Cutts for maintaining the Ensembl computer systems.
A second generation human haplotype map of over 3.1 million SNPs
The 1000 Genomes Project
A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome
TranscriptSNPView: a genome-wide catalog of mouse coding variation
SNP and haplotype mapping for genetic analysis in the rat
dbSNP: the NCBI database of genetic variation
Genome analysis of the platypus reveals unique signatures of evolution
Overlapping genomic sequences: a treasure trove of single-nucleotide polymorphisms
A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms
A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms
Ensembl 2009
The UCSC Genome Browser Database: update 2009
Variation resources at UC Santa Cruz
Database resources of the National Center for Biotechnology Information
The Ensembl Web site: mechanics of a genome browser
The Ensembl core software libraries
BioMart--biological queries made easy
Ensembl Genomes: extending Ensembl across the taxonomic space
Ensembl 2007
Ensembl 2008
Priorities for nucleotide trace, sequence and annotation data capture at the Ensembl Trace Archive and the EMBL Nucleotide Sequence Database
A database and API for variation, dense genotyping and resequencing data
SSAHA: a fast search method for large DNA databases
A haplotype map of the human genome
Whole-genome patterns of common DNA variation in three human populations
HGVbase: a human sequence variation database emphasizing data quality and a broad spectrum of data sources
The European Genome-phenome Archive
Potential etiologic and functional implications of genome-wide association loci for human diseases and traits
An SNP map of the human genome generated by reduced representation shotgun sequencing
Effects of PTPN22 C1858T polymorphism on susceptibility and clinical characteristics of British Caucasian rheumatoid arthritis patients
Haploview: analysis and visualization of LD and haplotype maps
Enredo and Pecan: Genome-wide mammalian consistency-based multiple alignment with paralogs
Genome-wide nucleotide-level mammalian ancestor reconstruction
Detection of large-scale variation in the human genome
Locus Reference Genomic sequences: an improved basis for describing human DNA variants.


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- Wi-Fi (802.11 b/g/n 2.4Ghz)
- Bluetooth 4.0
- Heart rate sensor
- Accelerometer
- Gyroscope
- Ambient light sensor
- Second-generation OLED Retina display with Force Touch (2x brighter)2
- Sapphire crystal (stainless steel)
- Ion-X glass (aluminum cases)
- Built-in rechargeable lithium-ion battery
- Up to 18 hours of battery life4
- Magnetic charging cable
Some bands are sold separately. Apple Watch requires iPhone 5 or later. Classic Buckle, Leather Loop, Modern Buckle, Milanese Loop, and Link Bracelet bands are not water resistant.
1 ISO standard 22810:2010. Appropriate for shallow-water activities like swimming. Submersion below shallow depth and high-velocity water activities not recommended.
2 Compared with the previous generation.
3 All models come in distinct case and band combinations. Sport Band, Woven Nylon, stainless steel, and leather bands are all compatible with all Apple Watch models.
4 Testing conducted by Apple in August 2016 using preproduction Apple Watch Series 1, Apple Watch Series 2, and Apple Watch Edition, each paired with an iPhone; all devices tested with prerelease software. All-day battery life is based on 18 hours with the following use: 90 time checks, 90 notifications, 45 minutes of app use, and a 30-minute workout with music playback from Apple Watch via Bluetooth, over the course of 18 hours. Battery life varies by use, configuration, and many other factors; actual results will vary.
TM and © 2017 Apple Inc. All rights reserved.
Compatible Devices
- Apple:
- Apple iPhone SE , Apple® iPhone® 6 Plus , Apple® iPhone® 6 , Apple iPhone 6s , Apple iPhone 7 Plus , Apple iPhone 5 , Apple iPhone 6s Plus , Apple iPhone 5s , Apple iPhone SE GoPhone , iPhone 6 GoPhone , Apple iPhone 7 , Apple iPad Pro (10.5-inch) , Apple iPhone 5s - 16GB $129.99 with the Purchase of $45 Airtime Card , Apple iPhone 6 Plus GoPhone , Apple iPhone 5s - 16GB $199.99 with the Purchase of $45 Airtime Card
Item SKU: 6302A(Apple Watch Series 2 - Silver Aluminum Case with White Sport Band - 42 mm)
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text: Presented by Prof Ahmad Al Khazim Al Ghamdi, Supervisor of Bagshan Bee Research, King Saud University The idea of this invention is to design a bee hive system with favorable conditions inside the beehive colony that is suitable for local environmental conditions. Due to high temperature and dryness, we lose almost 70% of our bees in some parts of Saudi Arabia in the summer. This system uses 7 frames (wax comb plates) and will be produced locally instead of the imported traditional one of having 10 frames. As research proved, eggs of the queen will not hatch if the humidity is less than 50%. Hence this system will be fully equipped with a humidity controller and temperature controller governed by software to control the humidity (50% to 70%) and temperature (below 34 or above 32) whenever needed in summer preventing bees from death of the colony.


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text: Directory Search Directory Go back to directory. Add to Address Book. Kurt Blalock 2nd Grade Teacher Swanton Local School DistrictCrestwood Elementary Work Email: firstname.lastname@example.com No Image Available Categories: Crestwood Elementary Updated 2 years ago. Categories Directory Categories Athletic Personnel Board of Education Members Crestwood Elementary District Staff Park Elementary Saint Richards Swanton High School Swanton Middle School Transportation


text:
posted by Amber .
A police radar has an effective range of 1.0 km, and a motorist's radar detector has a range of 1.9 km. The motorist is going 100km/h in a 80km/h zone when the radar detector beeps. At what rate must the motorist decelerate to avoid a speeding ticket?
Find: a
s = (1.9-1.0)[km]
u = 100[km/h]
v = 80[km/h]
Using two equations:
<1>: s = u t + ½ a t²
<2>: v = u + a t
Rearrange <2> to get t.
t = (v-u)/a
Substitute this into <1> to get a.
s = u(v-u)/a + (v-u)²/(2a)
.: a = (v²-u²)/(2s)
Calculate using the given values.
I suggest converting from [km/h²] to [m/s²]


text:
Fangoria and fuseTV have teamed to bring the world the first-ever televised Chainsaw Awards; this you know already. What you probably don't know is when or where they'll be shooting said awards, nor when they will be on the channel… and that information will come at a price…
For today only, though, it's free! The awards will take place on Sunday, October 15th (oddly, my wedding anniversary!), at the Orpheum Theatre in Los Angeles. The show will then be televised on fuseTV at 9:30 pm on October 22nd. Details as to how you can attend are forthcoming, don't you worry about that.
So what films are up for awards this year? Lots of good stuff, and the categories are pretty varied. Hostel leads the pack with six nominations while Snakes on a Plane, The Hills Have Eyes, and Underworld: Evolution all nabbed five apiece. Neil Marshall's The Descent, which had better fuckin' win for best film, comes in with four noms, which ain't too shabby either.
For the full list of who could win what and how to make your vote really count, click here!
Celebrate horror awards in our forums!


text:
Had a great time here. It self guided, we spend about two hours there. Great for all ages. Will go again.
Battleship Cove
5 Water Street
Fall River MA 02721
Fall River MA 02721
(508) 678-1100 Website
See Availability
Sponsored Topics
Opening Hours
- Mon: 9am-5pm
- Tue: 9am-5pm
- Wed: 9am-5pm
- Thu: 9am-5pm
- Fri: 9am-5pm
- Sat: 9am-5pm
- Sun: 9am-5pm
See a problem? Let us know!
I love the Battleship Cover and going down to the museums, all the Military services for Memorial Day, Veteran's Day and you can sleep over on the Battleship which is so much fun for guests,...
All the times we've driven home on I-195 from Cape Cod, I've wanted to stop and check out the fighting ships in Battleship Cove. There is a ton of history here, approximately 40,000 tons of it, if...
Amazing battleship USS Massachussetts, throughly enjoyed going on the ship and exploring the different inner parts of the ship along with the engine. Admission fee is 18$ for adults but there's...
It is totally weird how much I have become a walking commercial for this place since I went there with my two boys (10&14) and a friend visiting from Italy.We were there almost 4 hours. My boys...


text:
Dr. Roger Morales MD Patient Reviews
- Pediatrics Monroe, GA
Overall Rating
11 Patient Reviews
- Wait Time 15 minutes
- Easy Appointments
- Promptness
- Friendly Staff
- Fair and Accurate Diagnosis
- Bedside Manner
- Spends Time With Patients
- Appropriate Followup
Showing 1 - 11 reviews
4.0 of 5
March 29th, 2016
Dr. Morales is great I could not ask for better doctor however his staff is horrible, I have never had such a bad customer service and I have 2 kids in there for now over 11 years. At the end I have decided to find another doctor just b/c of his staff. To bad for him, I like him and I liked him when he took over from Dr. Lovett but I will not put up with his staff anymore and their bad attitude. :(
4.0 of 5
April 26th, 2009
Dr. Morales is wonderful. He really cares about his patients and takes the time to explain things to the parents. I only suggest that he get all new office staff. One of the office ladies was there when it was Dr. Rajwani's office and she should really change profession. They are not friendly and they do not follow thru.
5.0 of 5
March 7th, 2017
5.0 of 5
March 7th, 2017
5.0 of 5
February 7th, 2017
5.0 of 5
December 2nd, 2016
5.0 of 5
July 15th, 2016
4.0 of 5
May 24th, 2011
2.0 of 5
December 14th, 2010
5.0 of 5
September 8th, 2009
2.0 of 5
April 30th, 2009
Health Guides
More than 22 million Americans of all ages have asthma. Prepare to talk to your doctor about symptoms, diagnosis and treatment options.
Nearby Doctors
Dr. Roger Morales, MD is similar to these 3 Doctors near Monroe, GA.
Monroe, GA
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text:
Original French poster, large size 47x63 inches. With Diana RIGG, Patrick MACNEE. Two episodes of the TV series released in France, because they were too violent for the TV viewers. Same thing happened in Italy and for others TV series. This the style A.
Very good condition, folded, small wear and creases.


text:
Very knowledgeable about hobby. Nice selection of trains, model cars and planes, among other things. Friendly and helpful staff. I would recommend highly!!
Chicago, IL Hobby Shops
YP - The Real Yellow PagesSM - helps you find the right local businesses to meet your specific needs. Search results are sorted by a combination of factors to give you a set of choices in response to your search criteria. These factors are similar to those you might use to determine which business to select from a local Yellow Pages directory, including proximity to where you are searching, expertise in the specific services or products you need, and comprehensive business information to help evaluate a business's suitability for you. "Preferred" listings, or those with featured website buttons, indicate YP advertisers who directly provide information about their businesses to help consumers make more informed buying decisions. YP advertisers receive higher placement in the default ordering of search results and may appear in sponsored listings on the top, side, or bottom of the search results page.
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1. Harlan J Berk Ltd
I do not normally write reviews but I felt the need to write this when I saw how bad the reviews for this store were. Honestly, these people have b…
2. Chicagoland Hobby Inc
Very knowledgeable about hobby. Nice selection of trains, model cars and planes, among other things. Friendly and helpful staff. I would recommen…
3. Chicago Kite & Flags
I needed a gift for my 7 year old cousin, and wasn't really sure what to get him. I decided to stop in Chicago Kite, even though I have no knowledg…
4. Archie's
5516 W Devon AveChicago, IL 60646
From Business: Archie's has the best prices in Chicago and the North Shore and it's been that way since 1955. Throughout Chicagoland, when anyone needs top dollar for gold coins…
5. Forever Timeless Hobby Shop
4438 W Belmont AveChicago, IL 60641
6. Grayland Station
This place is simply stocked to the gills. Absolutely everything a self-respecting train nerd needs in N/HO scales is all right here. (Honest!) T…
7. Timeless Toys Ltd
I love this store. I'm so tired of all the electronics and cartoon and tv driven toys!! Timeless toys really allows you to let a child express the…
8. Mazel & Co
4300 W Ferdinand StChicago, IL 60624
From Business: Mazel & Company Inc. is a distributor of fasteners, wire products and building materials. Since 1919 it has been serving the wholesale and retail markets and is l…
9. Building Blocks Toy Store
3306 N Lincoln AveChicago, IL 60657
10. Horizon Hobby
200 W Adams StChicago, IL 60606
11. Stay Straight
2010 W Fulton St Ste F106aChicago, IL 60612
12. Needlecraft By Maria
1811 W 47th StChicago, IL 60609
13. Hobby 2 Habit Sports Hits
804 W Blackhawk StChicago, IL 60642
14. Karyn Kozak Design
6363 N Hermitage AveChicago, IL 60660
15. Ram Train & Hobby Shop
6603 W Higgins AveChicago, IL 60656
16. Building Blocks Toy Store
2130 W Division St Ste 1eChicago, IL 60622
17. Galactic Force Toys
1121 W Belmont Ave Ste 1Chicago, IL 60657
18. Infantino
566 W Adams St Ste 650, Chicago IL, 60661Chicago, IL 60661
19. Mixed Bag Collectibles & Crfts
8523 W Catherine AveChicago, IL 60656
20. Sister Arts Studio Inc
This is a really fun shop. I could spend hours in here. I purchased some great art acitivities for my nephews. No, it is not just for girls. …
21. Artist and Craftsman Supply
828 S Wabash AveChicago, IL 60605
22. Chazerly Designs Inc. DBA Crystal House
500 N Michigan Ave Ste 300Chicago, IL 60611
23. Wishcraft
3907 N Damen AveChicago, IL 60618
24. Jarvis Holdings Inc
4017 W Irving Park RdChicago, IL 60641
25. Her Vel's Unlimited Ceramics
8817 S Stony Island AveChicago, IL 60617
26. Lakeview Art Supply
3314 W Foster AveChicago, IL 60625
27. Battery Giant
4837 N Milwaukee AveChicago, IL 60630
28. A-1 Laser
4800 S Central AveChicago, IL 60638
29. M & Models
9239 S Cicero AveOak Lawn, IL 60453
30. Berwyn's Toy Trains & Models
7025 Ogden AveBerwyn, IL 60402
From Business: Located on historic Route 66, we are a specialty toy and hobby store. We have a 7'X 14' operating train layout, and a play area with three play tables. We sell O-…


text:
Upcoming Birthdays
(next 10 days)
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Lessons From A Galaxy Far, Far Away - You Will Be The Death Of Me
Posted by
March 22, 2014
at 07:44 AM CST |
?Don?t make me pull this car over.? ? Every Parent
?If I have told you once, I have told you a hundred times?What don?t you understand about the word NO?You are just like your father?What were you thinking?? If you have ever issued one of these statements (or statements like these), then this column is for you. How many times have these or similar statements been spoken by parents throughout the centuries? While there are times that can be trying, and days that can be frustrating, this journey of parenthood is one (on most days) we would never trade.
One of the things that was neat about the prequel trilogy was to see how the Jedi at their peak did things. We got to see them at the height of their power and ability. We saw the beauty of their majestic temple, and the way their lives were ordered. We saw the training and lifestyles they lived each and everyday.
One of my favorite scenes comes in Episode 2. From the beginning of the movie, you can see some of the tension that exists between Obi-wan and Anakin. A young teenage man just itching to be free of the constraints his master puts on him. While obedient, he challenges. While submissive, he argues for his perspective. Obi-wan is the ?old fart? who is out of touch and doesn?t quite understand. Anakin knows he loves him and cares for him, but sometimes feels he just does not get him (Any of this sound familiar to your house or childhood?).
As the story plays out, Anakin takes some ?unusual? risks and has a little ?unorthodox? style in how he handles some situations, which becomes a source of a frustration to Obi-wan. He and Anakin have been over this a hundred times in training. He has gone over and over these lessons, and Anakin just keeps rushing in, he continues to display impatience, and many times is just simply reckless.
As our two heroes engage in a speeder chase through the airways of Coruscant, Anakin takes a reckless course through a power coupling, and out of concern and frustration, Obi-wan utters, ?How many times have I told you?? (ever used a phrase like this). In the chase, Anakin takes a ?short cut? and manages to lose the assassin. You can almost hear the ?what were you thinking? in Obi-wan?s tone as he chastises Anakin for yet another mistake. Anakin then leaps from the speeder, and catches up (literally) with the criminal they were pursuing.
While fighting to bring the speeder down, Anakin manages to lose his lightsaber (which of course, Obi-wan catches). As Anakin and the assassin?s speeder crash, Obi-wan quietly and calmly lands his. With the criminal on the move, Anakin jumps up in pursuit, and meets a calm and somewhat frustrated Obi-wan at the door.
As he hands the lightsaber back to Anakin, you can almost hear the ?If I have told you once, I have told you a hundred times? speech coming. As he reminds Anakin AGAIN about the lessons he has learned, he hands the lightsaber back to him and utters that wonderful line, ?Why do I get the feeling you?re going to be the death of me.?
While this line foreshadows what will happen later in the saga, it rings true with so many parents, because most of us at one time or another have thought or said this very same thing about or to our children. Through frustrating events, or just having to tell them the same things over and over again, we wonder if they or we will make it to them becoming adults. As the exchange continues, Anakin shares that Obi-wan is the closest thing he has to a father, and Obi-wan responds with, ?Then why don?t you listen to me.?
I think conversations like these are ones that every parent, in every home around the world has had. So many days as parents we feel like we are simply a broken record, repeating the same lessons, same instructions, and same corrections over and over and over again, to little people who never seem to hear, understand, or have memory beyond a minute and a half. While Obi-wan was not Anakin?s ?father?, in reality, that is how he functioned. He was the only father Anakin had ever known.
Their relationship and interactions mirrored so many parental experiences and show us some things as parents we can take away in our own journey with our children. I am sure that Obi-wan saw much of his young self in Anakin. He saw the things that needed to be tempered and molded. He knew the mistakes and the consequences of the choices that Anakin was making. He had seen enough Jedi not learn these things and one way or another be brought to ruin.
Some of the frustration he felt, and we as parents sometimes feel, came from Anakin?s struggle to learn from his and others? experiences. We as parents sometimes do not understand how they cannot learn after the first time and why they have to be told over and over again. We forget sometimes that we are the same (just ask me how many speeding tickets I have gotten in my life).
We sometimes forget that at all ages, there are things we need to be reminded of. Kids attention spans are sometimes nanoseconds, and they will need many reminders. As parents, we need to be there to keep giving them that reinforcement, just as Obi-wan was..
As parents, we also see many things in our kids that are present in our own life. We see mistakes we have made and things that needed correction, and often those are the things we hit the hardest. The things that frustrate us about ourselves, are the things that often seem most evident in our children. When presented with this, it can also be a time for not just our children to learn, but for us as well. As we are guiding our children, sometimes we need to guide ourselves in these areas as well.
The nice thing about this relationship between Anakin and Obi-wan is that while there were days and events that were frustrating, Obi-wan always looked for the good in his padawan. He always took time to acknowledge his successes, and to share with him how proud he was. In this, we see a final lesson from this pair ? take time to notice the good.
It is easy to harp on all the things they do wrong, and notice all the issues they have and mistakes they make, but our children are more that just a collection of errors. In each one is talent, ability, caring, and a wonderful promise for the future. Our children need to feel our love and know how proud we are of them, just as Obi-wan tried to share with Anakin, and just as he continued to care for and love Anakin, no matter what.
So the next time your children are frustrating, and you think they will be the death of you, and you are telling them something for the hundredth time, remember Obi-wan and Anakin. Remember your children are probably more like you than you would like to admit, they will need many reminders throughout life, and while instructing them ?once again?, take time to remember the good in them. Just as quickly as you correct, tell them you love them and are proud of them. While you think your children will be the death of you, remember, your parents probably thought the same thing too ? from time to time.
Lessons From A Galaxy Far, Far Away is a regular column written by Charles Mickles for TheForce.Net for those who grew up on Star Wars, and now are sharing that experience with a younger generation.Star Wars offers many wonderful life lessons, and I hope these will help us better reach and impact those little ones that we each know and love as they take those first steps into that galaxy far, far away.
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text: Clockwise from top left: Getty; Getty; AP; GettyNo LoveSummer of Breakups: Kristen Stewart, Tom Cruise & More (PHOTOS)From Johnny Depp to Hilary Swank and Katy Perry, see celebrities who ended up single this summer.The Daily Beast08.28.12 4:45 AM ETClockwise from top left: Getty; Getty; AP; GettyClockwise from top left: Getty; Getty; AP; GettySummer of Breakups: Kristen Stewart, Tom Cruise, & More (PHOTOS)It's supposed to be a season of flirting and flings, but lovin' was in short supply this summer, with couples splitting from every corner of Hollywood. From Twipocalypse to the breakups that put Johnny Depp, Hilary Swank, and Henry Cavill back on the market, see the celebrities who ended up single. Josephine Santos / PacificCoastNews.comKaty Perry & John MayerWe could almost hear the collective sighs of relief from Katy Perry fans when Us Weekly reported last week that the pop star and John "Your Body Is a Wonderland" Mayer have gone their separate ways. The two had been spotted canoodling at parties around L.A. throughout the summer but it seems the relationship was just a summer fling. Best of luck to whoever's next on John's extensive hit list. Tony Gutierrez / AP PhotoHilary Swank & John CampisiShe ain't his million-dollar baby anymore. After five years together, Oscar winner Hilary Swank and her agent/boyfriend John Campisi have called it quits, a rep confirmed to People last week. The two began dating in December 2007, shortly after her divorce from actor Chad Lowe in November of the same year. Ian Gavan / Getty ImagesKristen Stewart & Robert PattinsonWith Breaking Dawn Part 2's release looming on the horizon—and the promise of exceedingly awkward promotional tours—the Kristen Stewart and Rupert Sanders cheating scandal just won't be dying down anytime soon. Ever since Stewart, 22, was caught kissing her married 42-year-old Snow White and the Huntsman director, the spotlight has remained on the actress and her now-estranged boyfriend of three years, Robert Pattinson. Regardless, the two will cozy up together onscreen in the upcoming conclusion to the Twilight saga. Adrian Sanchez-Gonzales, AFP / Getty ImagesTom Cruise & Katie HolmesThe only breakup bigger than Twi-gate this summer, Tom Cruise and Katie Holmes's divorce hit with the force of an atomic bomb. Why was she leaving now after five years? Who would Suri stay with? Was it because of Scientology? What is Scientology, anyway? Were they ever really in love? How big would her payout be? There was no shortage of questions surrounding the breakup of one of Hollywood's most famous couples, but the divorce played out at lightning speed. It took only two weeks for the ex-couple to reach an agreement, which reportedly gave Katie a relatively meager payout: she received no part of Tom's $250 million fortune and will be paid $400,000 a year in child support for the next 12 years, until Suri turns 18. Martin Bureau, AFP / Getty ImagesJohnny Depp & Vanessa ParadisWe were rooting for these two, but after 14 years and two kids together, Johnny Depp and French actress Vanessa Paradis officially announced their separation in June. Just two days before the announcement, Paradis sat down with French Elle and divulged some details about why, perhaps, she might be unhappy: "Sometimes you could be in an unhappy relationship; you are very much in love with someone, but it's making you unhappy and you think things can change and you can work it out." AP Photo; Getty ImagesJenny McCarthy & Brian UrlacherThis year's July/August Playboy cover girl, Jenny McCarthy had a six-month romance with Chicago Bears linebacker Brian Urlacher before announcing this summer: "Brian and I have decided to turn our romance into an amazing friendship. I will continue to be the biggest cheerleader for him. Go, Bears!" AP Photo; AP PhotoJohn Edwards & Rielle HunterFour years, one destroyed marriage, and one baby girl later, Rielle Hunter finally decided that her affair with former Sen. John Edwards was just not worth it anymore. Hunter began the now-infamous affair with the former presidential candidate during his 2008 campaign for president, when he was still married to his wife of 31 years, Elizabeth Edwards, who was dying of cancer. Hunter broke the news of her breakup in June during an appearance on Good Morning America, on the same day her memoir, What Really Happened: John Edwards, Our Daughter & Me hit bookstore shelves. Peter Kramer / Getty ImagesDenise Richards & Richie SamboraJune also saw the demise of the couple formerly known as Denise Richards and Bon Jovi guitarist Richie Sambora. The pair dated from 2006 to 2007, after Richards famously went through an ugly divorce with Charlie Sheen and Sambora split from Heather Locklear. The two then rekindled the romance last November—but it wasn't meant to be. "We aren't dating, but as a lot of people have seen, I stay very friendly with my exes. All of them," she said during an appearance on The Talk. Frazer Harrison / Getty ImagesKenny G & Lyndie BensonJazz saxophonist Kenny G filed for divorce this month from his wife of 20 years, Lyndie Benson-Gorelick, seven months after she originally filed for legal separation. The 56-year-old musician cited "irreconcilable differences" on the divorce documents and is seeking joint custody of the youngest of their two sons; the other is no longer a minor. Though Kenny G is worth $50 million, the two had a prenuptial agreement, according to the documents. Frazer Harrison / Getty ImagesDebra Messing & Daniel ZelmanSmash star Debra Messing filed for divorce in June from her husband of 10 years, actor and TV producer Daniel Zelman. The pair first met while they were students at New York University in the '90s and went on to date for eight years before tying the knot in September 2000. Documents listed the couple's date of separation as Feb. 15, 2010. Messing hinted at trouble in paradise in a recent interview with Ladies' Home Journal, saying: "I think the institute of marriage is a noble thing. I think the idea of a partner for life is incredibly romantic. But now we're living to 100. A hundred years ago, people were dying at age 37. 'Til death do us part was a much different ideal." Messing and Zelman are seeking joint custody of their 8-year-old son, Roman. David Livingston / Getty ImagesDanica McKellar & Mike VertaThe Wonder Years alum and math whiz extraordinaire Danica McKellar called it quits in June after three years of marriage to composer Mike Verta, with whom the actress has a 1-and-a-half-year-old son, Draco. The couple dated for eight years before finally tying the knot in a La Jolla, Calif., ceremony in 2009, but apparently it wasn't meant to be. Getty Images; Getty ImagesHenry Cavill & Ellen WhitakerWhen Henry Cavill, star of the upcoming Superman flick Man of Steel, proposed to show jumper Ellen Whitaker in February 2011, she was thrilled. "I couldn't believe it when he asked me to marry him," she later told the Belfast Telegraph that spring. "I thought we were there to celebrate his birthday. It was such a surprise and I am so happy." Fast-forward to this August, however, and the couple have reportedly called off their engagement. "They both just agreed it wasn't working," a source told Us Weekly. Charlie Neibergall / AP PhotoStevie Wonder & Kai Millard MorrisSixty-two-year-old music legend Stevie Wonder filed for divorce this month from his wife of 11 years, Kai Millard Morris, who is the mother of two of his children, 10-year-old Kailand and 7-year-old Mandla. The two met in 1999 at the club Nell in New York, were engaged within six months, and married in September 2001. For those wondering, Wonder signed the divorce papers using his legal name, Stevland Morris. Michael Buckner / Getty ImagesFred WillardActor Fred Willard didn't break up or get divorced this summer—he's been married to his playwright wife, Mary, for 40 years now—but he definitely got no love. Willard was arrested last month for allegedly engaging in a "lewd act" inside an adult theater, delightfully named the Tiki Theater, in Hollywood, and was promptly fired from his gig as the host of the PBS show Market Warriors.


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Thomas H Lee mulls buy of Yahoo US business
01 Dec 2011
The US buyout firm is circling Yahoo's US operations while rivals including Kohlberg Kravis Roberts are expected to bid next week for a stake in the broader company
Private equity firm Thomas H Lee Partners is pursuing a leveraged buyout of the US operations of Yahoo, in a deal which could be worth between $5bn and $6bn, Reuters reported Tuesday on its website, citing people familiar with the matter.
Arrange a group licence or trial
Private Equity News is sold on a group licence basis, to provide every member of a
Private Equity News is sold on a group licence basis, to provide every member of a
team with a weekly copy and access to the Private Equity News website.
You can arrange a trial for your team and receive details on how to set-up a group licence now.


text:
LODI - Chandler Bengston woke up Central Catholic's bats with a loud two-run dinger that sparked a game-breaking, two-out rally.
STOCKTON - Allie Walljasper had plenty to do with Sierra punching its ticket to the semifinal of the Sac-Joaquin Section Division IV softball tournament Wednesday night.
RIO LINDA – Teagan Manus scored a hat trick and Bryanna Heida earned the clean sheet as Ripon Christian moved within one win of its first Sac-Joaquin Section championship.
LODI – The Ripon Christian Knights used their 12-day layoff to get back to the fundamentals.
Sierra High's 4x100 boys relay team broke the Sac-Joaquin Section Division IV/V Meet record Tuesday on its home track.
Ashley Almeida dove to her left, batting a rocket shot out of bounds.
STOCKTON - There was no fist pump. No twirl of the putter. Not even a smile cracked the face of the always even-keel Will Brink.
On a championship-caliber team with no Sac-Joaquin Section championships, Jim Todd isn't leaning on a rich core of veterans for the missing ingredient.
Sierra High has built it.
Manteca and Ripon Christian begin the Sac-Joaquin Section Division playoffs against familiar foes next week.
The road to the Sac-Joaquin Section Division IV championship appears to go through the Valley Oak League once again.
Ripon High's Zach Scudder and freshman Scott Tolman of Sierra are two of the favorites in today's Sac-Joaquin Section frosh-soph final in the 100-yard breast stroke.
RIPON - It was two years ago on this same field that four current Ripon Christian seniors experienced heartache.
Meghan Wallace stood at the edge of the penalty area in stunned disbelief - hands atop her head, mouth agape.
MODESTO – Ripon's Yvette Serrano put together a respectable effort in the net Thursday afternoon at David Patton Field.
Page 187 of 422
MOUNTAIN HOUSE - Jonathan Borres served 15 unanswered points at one point in the second game, and the Manteca boys volleyball team rolled past Mountain House ...
Tawny Deerinck is a big part of Manteca High's resurgence in softball, and she aims to help head coach Ashley Carter turn things around ...
Page 8 of 8
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text:
Finance::Quant::TA - Perl extension for blah blah blah
Stub documentation for Finance::Quant::TA, created by h2xs. It looks like the author of the extension was negligent enough to leave the stub unedited. Blah blah blah. EXPORT None by default....SANTEX/Finance-Quant-TA-0.01 - 27 Jan 2012 08:02:34 GMT - Search in distribution
1 result (0.119 seconds)


text:
A Newcomer's Guide to Buying a Home in Canada
Buying a home is often at the top of the list of dreams for recent immigrants. However, they may not be familiar with what it takes to buy a new home in Canada. There are a number of resources available for anyone who is new to the country and looking to buy a home, such as a guide to affordable homes that have all the necessary features.
Many newcomers to Canada bring their families with them, so the necessary amount of space should be at the top of the home's "must-have" list. Detached homes offer a good option for families, regardless of their size. You should also take other factors into account when you buy a new home in Canada, such as how close it is to important facilities like shopping, schools, and your workplace. For instance, if you work in Downtown Toronto and don't want to put up with the hassle of driving into the city—not to mention paying for parking—you'll want to buy a home close to a GO station.
While researching the area you're looking to buy a home in can help, nothing beats actually going to the area and getting a feel for it. But while going with your heart when you're looking at affordable homes is great, there are other factors you'll also need to consider, such as:
This is probably the most important part of buying a home. While most newcomers to Canada come here well prepared, you'll still have to determine what you can afford. The amount of the mortgage will be the price of the house minus the down payment you made on the home. However, since you are new to the country, you may not have the credit history necessary to buy a new home in Canada. Consider working with a mortgage broker or lender to obtain the financing for a mortgage.
But don't fret, your bank can work with you to help you obtain a credit card and start building a credit history.
Hiring a Real Estate Agent
When it comes to finding an affordable home, you should leave it up to the professionals by hiring a real estate agent to take care of tasks such as finding a home and negotiating a price. Your agent will also handle negotiating an offer on the home you ultimately decide on.
Hiring a Home Inspector
If you're looking at buying a home, you should hire a home inspector to look it over so you can determine any issues before you move in.
If you have any family who are already living in Canada and in the area you are planning to move to, ask them for recommendations regarding real estate agents, lawyers, home inspectors and other professionals. Quality professionals can also be found using online directories and reviews. You can also visit the Canadian Mortgage and Housing Corporation for resources for newcomers to Canada looking to buy a home.
Bremont Homes offers affordable homes in communities throughout Southern Ontario, an area popular with newcomers. If you're looking to buy a new home in Canada, including luxury detached homes close to all the amenities, contact us today!
Chih, V., "New to Canada and buying a home?" The Ottawa Sun, November 7, 2014; http://www.ottawasun.com/2014/11/07/new-to-canada-and-buying-a-home.


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Shops near Mahiki
London > Shopping > House and Garden
9 Albemarle Street, Mayfair, London W1S | 1 minute from Mahiki
Paul Smith is the indisputable don of British fashion. Launched in a tiny shop in Nottingham in 1970 he is now a global brand.... More
London > Shopping > Jewellery
5 The Royal Arcade 28 Old Bond Street, Mayfair, London W1S | 2 minutes walk from Mahiki
Angela Hale boasts contemporary jewellery (notably Art Deco and 50s styles) coupled with a magnificent general selection from hair clips to tiaras. There are also... More
London > Shopping > Perfume and Cosmetics
12 The Arcade, 28 Old Bond Street, Mayfair, London W1S | 2 minutes walk from Mahiki
The luxury perfume company Ormonde Jayne, established in 2001, opened this tiny shop - a Caulder Moore-redesigned store - in the picturesque Royal Arcade off... More
London > Shopping > Perfume and Cosmetics
16-17 Burlington Arcade, Mayfair, London W1J | 2 minutes walk from Mahiki
130-year-old scent emporium with royal warrants. For a sweet-smelling, sensory overload, Penhaligon's has to be the place to go. From the original Hammam Bouquet to the... More
London > Shopping > Food and Drink Shops
1 Royal Arcade, Old Bond Street, Mayfair, London W1S | 2 minutes walk from Mahiki
In 1875, at the behest of Edward VII, Mme Charbonnel quit her post at Parisian chocolatier Maison Boissier to set up a fine confectionary house... More
London > Shopping > Gifts and Luxuries
60 Burlington Arcade, Mayfair, London W1J | 2 minutes walk from Mahiki
As Mont Blanc's opulent pens are not officially available over the internet, you will have to visit one of their special boutiques in order to... More
London > Shopping > Shoes
Burlington Arcade, W1J | 2 minutes walk from Mahiki
More than 40 years after Manolo Blahnik opened his first store on Old Church Street in Chelsea, the... More
London > Shopping > Jewellery
12a Piccadilly Arcade, St James's, London SW1Y | 2 minutes walk from Mahiki
Fine, modern, own-design jewellery, using 18-carat gold and gemstones. In addition there is a selection of antique fine jewellery from 1850-1950. A catalogue is available... More
London > Shopping
French Railways House, 178-180 Piccadilly, St James's, London W1J | 2 minutes walk from Mahiki
Opened in December 2013, marking 20 years since her first Holland Park shop opened, this Piccadilly store is Cath Kidston's largest shop to date. Located... More
London > Shopping > Delicatessens
16 Lansdowne Row, Berkeley Square, Mayfair, London W1J | 3 minutes walk from Mahiki
Sweet to taste, cute to look at and made with natural ingredients, Lola's Cupcakes can hold their own when it comes to the coveted title... More
London > Shopping > Food and Drink Shops
45-46 Piccadilly, Mayfair, London W1J | 3 minutes walk from Mahiki
Extremely upmarket Piccadilly branch of the world-renowned Parisian chocolatiers, La Maison Du Chocolat. The French speaking staff at the beautifully decorated London store, all marble... More
London > Shopping > Food and Drink Shops
14 Princes Arcade, St James's, London SW1Y | 3 minutes walk from Mahiki
With Roald Dahl and Her Majesty counted as just a couple of their biggest fans, this brightly-coloured, richly scented, teeny tiny chocolate shop tucked away... More
London > Shopping > Arts and Crafts
8 King Street, St James's, London SW1Y | 3 minutes walk from Mahiki
Established in 1776, Christie's holds sales ranging from Old Masters to the contemporary arts. In 1990 it broke all records with the sale Van Gogh's... More
London > Shopping > Hair and Grooming
71 St James Street, St James's, London SW1A | 4 minutes walk from Mahiki
As any gentleman knows, there are times when only a shave by the finest of barbers will do. It's a bit like going to the... More
London > Shopping > Delicatessens
93 Jermyn Street, St James's, London SW1Y | 4 minutes walk from Mahiki
Specialising in French and English artisan cheeses, this historic venue served as cheesemonger to HM Queen Victoria. Pick up a selection of finest quality cheeses... More
Hotels Near
What's On Today
Near Mahiki
Royal Academy Summer Exhibition
Exhibitions | Until 20th August 2017
Royal Academy of Arts 2 minutes walk from Mahiki
Anyone can submit to the Royal Academy's Summer Exhibition which gives it a wonderful element of surprise. Over 12,000 pieces ...More
Green Ink Writers' Gym with Rachel Knightley
Exhibitions | On 25th June 2017
Waterstone's - Piccadilly 5 minutes walk from Mahiki
Boost your confidence - and your word count! Writers of all genres and levels of experience will develop new material ...More
Cool Things
Theatre | On 25th June 2017
Brasserie Zedel 6 minutes walk from Mahiki
Stacy Mitchhart
Concerts | On 25th June 2017
Brasserie Zedel 6 minutes walk from Mahiki
Theatre | Until 6th January 2018
Piccadilly Theatre 6 minutes walk from Mahiki
It's a hard-knock life in 1930s New York during the Great Depression when Miranda Hart makes her West End debut ...More
Exhibitions | Until 11th August 2017
Gagosian Gallery Davies Street 7 minutes walk from Mahiki
Exhibitions & Art, Sculpture....More
The Phantom of the Opera
Theatre | Until 14th October 2017
Her Majesty's Theatre 8 minutes walk from Mahiki
Andrew Lloyd Webber's most enduring hit has been running since 1984 and is still as popular with audiences and critics ...More
Comedy Store Players
Comedy | On 25th June 2017
Comedy Store 8 minutes walk from Mahiki
Improvised fun from Paul Merton, Andy Smart, Lee Simpson, Neil Mullarkey, Steve Edis and Josie Lawrence....More
Theatre | Until 2nd September 2017
Harold Pinter Theatre 8 minutes walk from Mahiki
Starring Juliet Stevenson and Andrew Scott, Hamlet transfers from the Almeida Theatre to the Harold Pinter Theatre this June. Juliet ...More
The Ferryman
Theatre | Until 7th October 2017
Gielgud Theatre 9 minutes walk from Mahiki
Following a sold out run at the Royal Court, Jez Butterworth's The Ferryman comes to the West End from June ...More
New on LondonTown
Nobu Shoreditch
At the heart of Nobu Hotel Shoreditch, this stunning 240-seat restaurant is the place to ...More
VQ Aldgate
Famous for staying open 24 hours VQ is a great spot, day or night, for ...More
Shikumen Aldgate
Shikumen at Dorsett City follows in the footsteps of its flagship restaurant at Dorsett Shepherds Bush, which has received Bib Gourmand from the Michelin guide inspectors....More
The Ivy Tower Bridge
Part of the Ivy's brasserie style restaurants, this one has a rather good riverside location ...More
Tom Simmons
Tom Simmons, a former star of MasterChef: The Professionals, dishes up cockle popcorn, laverbread mayonnaise ...More
Rola Wala
After the success of Street Feast residencies Rola Wala serves up authentic, healthy, every day ...More
Tibits Bankside
Displaying over vegetarian 40 dishes, tibits retains its showcase centrepiece at its second London restaurant....More
The Oystermen Seafood Bar and Kitchen
The Oystermen, aka Matt Lovell and Rob Hampton, ...More
Modern brasserie in Camden Market with Lee Andrews, formerly of Chiltern Firehouse, as head chef ...More
Hampstead Beach
Seasonal | From 2nd July
Summer's coming to Hampstead with an urban beach at JW3 with golden sand, free entry, ...More
Waiting for Godot
Theatre | From 5th September
Samuel Beckett's controversial Waiting for Godot comes to the Arts Theatre in September. The show sees Didi and Gogo and their aching feet pass the time playing games near a tree while they wait for ...More
Venus In Fur
Theatre | From 6th October
David Ives' hit Broadway show Venus in Fur opens in the West End this August, ...More
The Majority
Theatre | From 11th August
National Theatre: Dorfman Theatre
Writer and performer Rob Drummond follows on from his acclaimed production Bullet Catch with another ...More
Best Beaches in London
There's need to leave town when you're in search of sand, feel the grains ...More
The Revolution will be Photocopied
Exhibitions | On 1st July
Create your own protest publication in a zine-making ...More
Plywood: Material Of The Modern World
Exhibitions | From 15th July
Victoria and Albert (V & A) Museum
From hatboxes to tea chests, surfboards to aeroplanes, ...More
The City Is Ours
Exhibitions | From 14th July
More than half the world's population live in urban areas and that percentage is even ...More
Art Night London 2017
Exhibitions | On 1st July
Stay up all night on Saturday 1st July for Art Night, a night of free ...More
Eid Festival 2017
Exhibitions | On 2nd July
Where Christians have Christmas, Muslims have Eid - which gives you an idea of how ...More
Barnes Film Festival
Film Events | From 29th September
The Barnes Film Festival returns for a second year in 2017, offering an alternative to the central London film festivals dominating the scene. The festival brings together the great and good of south west London's ...More
Best Places for Street Food in London
London's street food scene has really taken ...More
September in London 2017
The Wellcome Collection explores the relationship between graphic design and health, The London Design ...More
London Hotels
From cheap budget hotels to luxury 5 star suites see our special offers for Mahiki hotels, including DoubleTree by Hilton Hotel London - Chelsea from 62% off.


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Sachin a Billion Dreams is one of the most awaited sports biopics at present and recently AR Rahman, the music director of the flick, released a single on Sachin Tendulkar's birthday.
Now sources reveal that as another treat to the fans, Sachin anthem which features in the movie will be released tomorrow by the 'Isaipuyal' and the 'master blaster'.
The flick will be hitting the screens on 26 May and the release of the Sachin Anthem is expected increase the hype for the film.
Meanwhile in Kollywood, Rahman is currently busy with 2.0, Sarvam Thaala Mayam and other projects.


text:
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text:
Dim Light is an android Adventure game. Download Dim Light Apk 2.0 free from Apkmoder.
Can you be the one to escape from this pitch-black madness?
Horror adventure game, "Dim Light"
Featured in Busan Indie Connect(BIC) Festival
I may be the last human being who figures this situation is "unnatural.."
And I'm pretty sure that I'm right.
Others seem uninformed about all of this.
The dark quietly hid itself and made creepy noises.
Finallly they brought "them," and they darkened the entire hospital with madness.
Today, I will escape from this hell.
The dim light shining from very far ahead will eventually save my life.
Download Link:
Dim Light Apk Download Link