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1Department of Cell Biology, Harvard Medical School
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The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers. This assay models the early steps of ovarian cancer metastasis.
Date Published: 2/17/2012, Issue 60; doi: 10.3791/3888
Keywords: Medicine, Issue 60, Ovarian Cancer, Metastasis, In vitro Model, Mesothelial, Spheroid
Davidowitz, R. A., Iwanicki, M. P., Brugge, J. S. In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis. J. Vis. Exp. (60), e3888, doi:10.3791/3888 (2012).
Ovarian cancer is the fifth leading cause of cancer related deaths in the United States1. Despite a positive initial response to therapies, 70 to 90 percent of women with ovarian cancer develop new metastases, and the recurrence is often fatal2. It is, therefore, necessary to understand how secondary metastases arise in order to develop better treatments for intermediate and late stage ovarian cancer. Ovarian cancer metastasis occurs when malignant cells detach from the primary tumor site and disseminate throughout the peritoneal cavity. The disseminated cells can form multicellular clusters, or spheroids, that will either remain unattached, or implant onto organs within the peritoneal cavity3 (Figure 1, Movie 1).
All of the organs within the peritoneal cavity are lined with a single, continuous, layer of mesothelial cells4-6 (Figure 2). However, mesothelial cells are absent from underneath peritoneal tumor masses, as revealed by electron micrograph studies of excised human tumor tissue sections3,5-7 (Figure 2). This suggests that mesothelial cells are excluded from underneath the tumor mass by an unknown process.
Previous in vitro experiments demonstrated that primary ovarian cancer cells attach more efficiently to extracellular matrix than to mesothelial cells8, and more recent studies showed that primary peritoneal mesothelial cells actually provide a barrier to ovarian cancer cell adhesion and invasion (as compared to adhesion and invasion on substrates that were not covered with mesothelial cells)9,10. This would suggest that mesothelial cells act as a barrier against ovarian cancer metastasis. The cellular and molecular mechanisms by which ovarian cancer cells breach this barrier, and exclude the mesothelium have, until recently, remained unknown.
Here we describe the methodology for an in vitro assay that models the interaction between ovarian cancer cell spheroids and mesothelial cells in vivo (Figure 3, Movie 2). Our protocol was adapted from previously described methods for analyzing ovarian tumor cell interactions with mesothelial monolayers8-16, and was first described in a report showing that ovarian tumor cells utilize an integrin –dependent activation of myosin and traction force to promote the exclusion of the mesothelial cells from under a tumor spheroid17. This model takes advantage of time-lapse fluorescence microscopy to monitor the two cell populations in real time, providing spatial and temporal information on the interaction. The ovarian cancer cells express red fluorescent protein (RFP) while the mesothelial cells express green fluorescent protein (GFP). RFP-expressing ovarian cancer cell spheroids attach to the GFP-expressing mesothelial monolayer. The spheroids spread, invade, and force the mesothelial cells aside creating a hole in the monolayer. This hole is visualized as the negative space (black) in the GFP image. The area of the hole can then be measured to quantitatively analyze differences in clearance activity between control and experimental populations of ovarian cancer and/ or mesothelial cells. This assay requires only a small number of ovarian cancer cells (100 cells per spheroid X 20-30 spheroids per condition), so it is feasible to perform this assay using precious primary tumor cell samples. Furthermore, this assay can be easily adapted for high throughput screening.
1. Ovarian Cancer Cell Spheroid Formation
2. Mesothelial Cell Monolayer Formation
3. Mesothelial Cell Clearance Assay
4. Representative Results
In this example, we compared the mesothelial clearance ability of OVCA433 ovarian cancer cell spheroids that have attenuated expression of talin-1 to control OVCA433 spheroids. OVCA433 spheroids from each group were added to a MatTek dish containing ZT mesothelial cell monolayers. Six spheroids from each group were imaged every 10 minutes for eight hours (Figure 4, Movie 3, Movie 4). The holes produced in the monolayer by the spreading spheroids were measured and six positions from each group were averaged. Figure 4 shows that the average clearance area created by talin 1 knockdown spheroids was significantly smaller than the average area created by control spheroids, suggesting that talin is required for mesothelial clearance by OVCA433 ovarian cancer spheroids.
Figure 1. Ovarian Cancer Metastasis. Primary ovarian tumors develop either from the ovarian surface epithelium or fallopian tubes. Tumor cells/ clusters break off from the primary tumor and collect in the peritoneal cavity. Tumor cells can then aggregate to form multicellular spheroids. Spheroids then attach to the mesothelial cell monolayers lining the peritoneal cavity. The mesothelial cells are excluded from underneath the attached ovarian cancer spheroid, allowing the spheroids to gain access to the underlying basement membrane.
Movie 1. Ovarian Cancer Metastasis. Click here to view movie.
Figure 2. Mesothelial cells line the surface of human peritoneal tissue and are excluded from underneath ovarian cancer cell implants.
Figure 3. Mesothelial Clearance Assay. Ovarian cancer spheroids are formed by incubating 100 RFP-expressing ovarian cancer cells per well in a poly-HEMA coated 96 well round bottom culture dish at 37°C for 16 hours. Poly-HEMA prevents the cells from attaching to the culture dish, allowing the cells to remain in suspension and adhere to one another to form a single cluster per well. Mesothelial cell monolayers are prepared by plating 6x105 mesothelial cells per well in a fibronectin coated 6 well MatTek dish and incubating the plate at 37°C for 16 hours. The spheroids are then transferred to the MatTek dish with the mesothelial monolayer and the two cell populations are imaged every 10 minutes for 8 hours using a Nikon Ti-E Inverted Motorized Widefield Fluorescence time-lapse microscope and Elements software.
Movie 2. Mesothelial Clearance Assay. Click here to view movie.
Figure 4. Attenuation of talin 1 expression in OVCA433 spheroids decreases mesothelial clearance ability. OVCA433 spheroids (red) with and without attenuated expression of talin 1 were allowed to attach to and invade into a ZT mesothelial monolayer (green). The two cell populations were imaged every 10 minutes for 8 hours using a Nikon Ti-E Inverted Motorized Widefield Fluorescence time-lapse microscope and elements software. The graph shows that talin 1 attenuation significantly decreases mesothelial cell clearance (Quantile plot with green bars at the means).
Movie 3. Control OVCA433 spheroids (red) invading into a mesothelial monolayer (green). Click here to view movie.
Movie 4. Attenuation of talin 1 expression in OVCA433 spheroids (red) decreases mesothelial (green) clearance ability. Click here to view movie.
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The "Mesothelial Clearance Assay" presented here uses time-lapse microscopy to monitor the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers, in great spatial and temporal detail. Previously, several groups8-14 had used endpoint assays to show that ovarian cancer cells attach to and invade into mesothelial cell monolayers. This assay is unique in that it uses fluorescently labeled cells to distinguish tumor cells from mesothelial cells, so that the dynamics of these two cell populations can be monitored throughout the assay. The process of intercalation can be visualized in real time and the rate of mesothelial clearance can be quantitatively measured over time. The use of timelapse microscopy allows one to closely monitor the dynamics of the interaction between the two cell populations under different experimental conditions. Additionally, a small percentage of either the mesothelial cells or the ovarian cancer cells can be labeled with a third fluorescent marker to monitor the dynamics of individual cells within the population. By tracking individual cells over time, the directionality and rate of migration can be calculated. To perform higher resolution analyses of mesothelial clearance, total internal reflection fluorescence (TIRF) microscopy can be used. If focal adhesions are labeled in the mesothelial cells, the dissociation of mesothelial adhesions by protrusive extensions of the tumor cells can be monitored, as described in our previous publication17.
This assay can be used to compare the invasion ability of ovarian cancer cell spheroids that have been genetically or pharmacologically modified to elucidate the molecular mechanisms by which ovarian cancer cell spheroids clear the mesothelial monolayer or to identify small molecule inhibitors of the process. Furthermore, the assay requires a very small number of ovarian cancer cells, so primary tumor cells from fluid exudates can be used (see limitations below) if labeled by preincubating the cells with cytotracker dyes (Invitrogen). The assay is also amenable to high throughput analysis. To perform high throughput genetic or pharmacological studies, the ovarian cancer cells can be transfected with different siRNA vectors or treated with different pharmacological inhibitors in each well of the 96-well plate Mesothelial cell monolayers can be plated in 96 well glass-bottom culture dishes, and the spheroids can be transferred 1:1 from the poly-HEMA coated plates to the monolayer-containing plates. All of these steps can be optimized for use with screening robots so that hundreds of siRNAs or inhibitors can screened at one time.
One of the strengths of this assay is to be able to model the force-dependent intercalation of ovarian cancer cells into the mesothelial monolayer. Our lab used traction force microscopy (TFM) to determine whether mechanical force regulates mesothelial clearance17. We found that overexpression of α5 integrin increased the contractility of cells plated on a fibronectin-coated substrate, while RNAi-mediated knockdown of talin, or myosin II decreased cell contractility17. Since downregulation of α5 integrin, talin, or myosin II in the ovarian cancer cell spheroids also decreased mesothelial clearance, our TFM measurements support the idea that mesothelial clearance in an event dependent on cellular contractile forces, in which cells with higher contractile forces caused greater mesothelial clearance. Therefore, the mesothelial clearance assay can be used to further understand intercalation events, associated with ovarian spheroid metastasis, that are dependent on mechanical forces.
This assay has a few limitations to consider. First, in order to form the multicellular spheroids, the cells must be cultured in suspension for at least 6 hours. If the cells are unable to survive without matrix contact their clearance ability will be compromised. Second, it is advantageous to use ovarian cancer cells that form uniform, compact multicellular spheroids. If the ovarian cancer cells only form loose clusters, they may break apart in the transfer from the polyHEMA-coated plate to the dish containing the mesothelial monolayer, creating spheroids of different shapes and sizes that will add variability to the data. Third, if the cells used are heterogeneous, this will add additional variability to the sizes of holes created in the monolayer. It is important to use multiple replicate wells (10-20/sample) due to the variability in extent of intercalation after eight hours. In the assays described here, well-established ovarian cancer (OVCA433) and mesothelial (ZT) cell lines were used. To make this assay more clinically relevant, primary ovarian cancer cells, from the ascites fluid of patients, can be used. It would be interesting to determine if in vitro mesothelial cell clearance ability correlates with clinical outcome. The limitations above are particularly important to consider when using primary samples, as the number of primary cells available is a limiting factor. Additionally, it is important to check the integrity of the mesothelial monolayer before performing this assay. The mesothelial monolayer can be fixed and stained for cell-cell junction proteins to ensure that mesothelial cell junctions are intact.
Finally, the mesothelial clearance assay can be easily modified to answer specific experimental questions. Here, we used fibronectin as the ECM component that allows the ovarian and mesothelial cells to adhere to the glass-bottom culture dishes, however, other ECM components can be used including collagen and laminin. Furthermore, other cell types that are found under the basement membrane, including fibroblasts, can be added to this experimental system, to assess the role of these cell types in mesothelial clearance9,19,20. Lastly, interactions of other types of tumor cells (e.g. pancreatic, breast, etc) with mesothelial cells can also be modeled using this assay. And it is feasible to study interactions between cancer cells and an endothelial monolayer, using this assay, to mimic intravasation or extravasation (Similar assays have been described in: 15,16,21-27 ).
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We have nothing to disclose.
We would like to thank the Nikon Imaging Center at Harvard Medical School, specifically Jennifer Waters, Lara Petrak and Wendy Salmon, for training and the use of their timelapse microscopes. We would also like to thank Rosa Ng and Achim Besser for valuable discussions. This work was supported by NIH Grant 5695837 (to M. Iwanicki) and GM064346 to JSB; by a grant from Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (to JSB).
|Catalog Number
|OVCA433 Ovarian Cancer Cells
|Gift from Dr. Dennis Slamon
|ZT Mesothelial Cells
|Gift from Dr. Tan Ince
|Medium 199
|GIBCO, by Life Technologies
|Cell Applications Inc.
|FBS-heat inactivated
|GIBCO, by Life Technologies
|GIBCO, by Life Technologies
|96 well plates
|Polyhydroxyethylmethacrylate (poly-HEMA)
|For poly-HEMA solution dissolve 6mg poly-HEMA powder in 1ml of 95% EtOH
|Dilute to 95% in dH20
|Cell culture hood
|Tissue culture incubator
|Thermo Fisher Scientific, Inc.
|incubator for poly-HEMA plates
|Labline Instruments
|Imperial III 305
|Tabletop centrifuge
|Heraeus Instruments
|6 well glass-bottom dish
|MatTek Corp.
|Nikon Instruments
|Ti-E Inverted Motorized Fluorescence time-lapse microscope with integrated Perfect Focus System
|Nikon Instruments
|20X-0.75 numerical apeture
|Halogen transilluminator
|Nikon Instruments
|0.52 NA long working distance condenser
|Excitation and emission filters
|Chroma Technology Corp.
|GFP Ex 480/40, Em 525/50 RFP-mCherry Ex 575/50 Em 640/50
|Transmitted and Epifluoresce light path
|Sutter Instrument Co.
|Smart Shutters
|Linear-encoded motorized stage
|Nikon Instruments
|Cooled charged-coupled device camera
|Hamamatsu Corp.
|Microscope incubation chamber with temperature and CO2 control
|Custom Made
|Vibration isolation table
|NIS-Elements software
|Nikon Instruments
|Version 3
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Hypothetico-nomological aspects of medical diagnosis Part I: General structure of the diagnostic process and its hypothesis-directed stage
- Cite this article as:
- Doroszewski, J. Metamedicine (1980) 1: 177. doi:10.1007/BF00883113
In medical diagnostic examination three main stages may be distinguished: (a) initial exploration, (b) hypothesis-directed investigation, and (c) final diagnosis making. The purpose of this work is to study some methodological problems concerning the second of the above stages of the diagnosis and to prepare a background for a mathematical model [30] of this process.
In diagnostic problem solving, the reasoning proceeds along the main lines traced by some initial suggestions and passes through various intermediate elements which are connected with one another forming ramifying chains and nets of inferences and hypotheses. Such a complex mental construction is based on laws which form medical knowledge and reflect various regularities and relations, causal, structural, functional, and others.
The main components of diagnostic reasoning may be divided into several classes according to their function and content: leading hypotheses, working hypotheses, main diagnostic hypotheses, statements accepted as certain, intermediary and reserve hypotheses, therapeutic suggestions of immediate consequence. In an example of diagnostic problem solving these types of propositions are defined and analysed.
In diagnostic reasoning, as in every other process of rational problem solving, explanation of the observed symptoms and signs and testing of the explaining hypotheses play a predominant role. These procedures form successive, frequently numerous and diversified steps and stages of the reasoning, leading to the construction of a mental model of the patient's state. Some problems relative to the scheme of explanation, especially to that which is based on causal laws, are discussed.


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Affectionately Captures $22,850 Fashion Stakes at Aqueduct by 2 Lengths; FASHION VERDICT FINISHES SECOND Affectionately Is Victor in 0:58 4/5 for Five Furlongs --Princess Babu Third Speedwell Is Slow Negro Minstrel Wins
By JOSEPH C. NICHOLS ();
May 17, 1962,
, Section Sports, Page 44, Column , words
Mrs. Ethel D. Jacobs' Affectionately endeared herself to the favorite players at Aqueduct yesterday by taking the $22,850 Fashion Stakes.
May 17, 1962
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Redlined Documents 101 is the page that gets you on your way in redlining contracts. You'll find answers to redlining questions like:
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text: Jan 01, 2010 - The Lavender is my first foray into the Wen world of flavors. I consider myself a product junkie and most recently was using CHS Silk Protein line. After experimenting I'm thrilled with a combo routine: cleansing with Lavender cleaning cream, using the CHS Silk Leave-in and follow with Curl Keeper. When I used the Wen as a leave-in I got too much poofiness and frizz. My hair is something between 3b and 3c and the Wen is about the best curl formation I've had: soft, loose, bouncy and well-defined curls! Take a leap and try the Wen cleansing creams--don't let the 'cones scare you.


text: Home > How To > Electrical > To Bond or Not to Bond? Electrical Q&A To Bond or Not to Bond? By George Flach Login or Register to download the PDF version of this article. (132.31 kB) A.George Flach, former chief electrical inspector for New Orleans, responds: Gas pipes often supply equipment and appliances that are also powered electrically — such as gas-electric central heating furnaces and gas heaters with pumps used for warming water in swimming pools, or gas clothes dryers and gas ranges with illuminated ovens and spark igniters — and they may become energized by the branch circuits that power these units.To reduce that risk, Section 250.104(B) of the 2005 NEC requires that all gas pipe be bonded. However, the NEC also recognizes the equipment grounding conductor of each branch circuit as a bonding means for the gas piping, and requires no additional bonding or grounding. Login or Register to continue reading


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'This race is little more than a slow motion train wreck…. a slow motion train wreck…' That was the overriding thought that stayed in my brain for much of the first 20 miles of the 2012 Steamboat Run Rabbit Run 100. So much for positive thinking. In fact, you could say I was down-right negative. Mentally, I had no business being out there. I felt horrible and was wondering what in the heck I was doing in a race like this. I am not certain why my race started out so poorly, but in retrospect I am shocked that my 'train wreck of a race' turned into a runaway train of energy and 'beast-mode' running. It felt like I went from hobby jogger to serious competitor with a snap of the fingers. What turned this race around for me? Not sure, but it all started with Marty Wacker.
Marty and I have known each other for a few years now as we have raced many of the same races throughout Colorado. I don't have the stats in front of me, but I have been fortunate enough to get the best of Marty on a few occasions and I know he has beaten me to the finish line more than once. Largely I have known Marty as 'the-really-nice-dude-who-can-get-into-a-groove-and-beat-you'. During the Run Rabbit Run 100 (RRR 100) I caught up to Marty about half way down the first major descent coming down the Fish Creek Falls Trail and he and I ran together for the final 3+ miles to the high school at mile 23. We chatted about the course, the weather, camping, what the winning time was going to be, etc. We also traded a few stories about getting lost in previous races. The part of the talk I remember the most though was about raising children. Marty has two teenagers and I pestered him for advice about raising a daughter. He gave me a few positive gems of info, we shared a good laugh, and as we neared the high school aid station, I think we parted ways with a solid fist-bump and swapping lines like: "Good to chat with you brotha', and I hope to see more of you throughout the race."
And with that 3 mile conversational exchange with Marty, positivity once again ruled my brain for the remainder of the race. Amazing how that works. It also helped that a mere one mile beyond the high school aid station I was going to see Annie and Jordan! This was tremendously exciting, and I came into Howelsen Hill (mile 24) ready for a bit of aid and to soak up more energy from Annie, Jordan, and our friends Dan and Becky. They were all there as I sat, ate some food, drank a bit, and reloaded for the next 20 mile stretch on Emerald Mountain. I left this aid station in good spirits, rolled up Howelsen Hill and the 'Lane of Pain' to the top of Emerald Mountain which led to a fantastic single track section at about 7800′. The sunset was beautiful, my energy was high, and I was beginning to pick off some people. I had initially been somewhere in the upper teens through the first two aid stations but had crept into 11th place at the Howelsen Hill aid station at mile 24. As I came back to the base of Howelsen Hill at what was supposed to be mile 40, I had moved into 9th place and was feeling good heading into the full darkness of night. One disconcerting note here was that my GPS was reading about 46 miles at what was supposed to be the mile 40 aid station. Yikes. This would be a trend that everyone was experiencing.
I sat for a few minutes as Annie provided me with the best aid in the world… new bottles filled with EFS from First Endurance, a few cups of coke, and a tremendous concoction of sweet-potatoes and deli-turkey. I gave Jordan a kiss as she was held and cared for by another Annie, (thanks!) and then grabbed my UltrAspire Spry vest, an iPod, as well as a handful of potato chips, and rallied to the high school and beyond. My mind had completely made the shift from running to racing and as I began the long climb back up the Fish Creek Falls Trail I began to move steadily faster, with a bit more effort, purpose, resolve, and bounce. My legs felt great and my range of motion, which had been limited all race so far, began to open up. I was feeling good as I embraced the loud music, sipped more EFS, and hammered up the Fish Creek Falls Trail and back to the Long Lake Aid Station. Once there I found out that in fact I was in 6th place overall. Whoa… really!? I know I had passed Mike Wolfe, but where did the others go? (Turns out many folks had taken the wrong drainage back up out of town and had wandered off course, added on too much distance, or dropped out).
I powered on along a smooth dirt road at approximately 10,000′ and cruised into the Summit Lake Aid Station, which was supposed to be mile 55, but my GPS was reading over 62 by this point! After a bit more aid consisting of mashed potatoes, I ran extremely hard down Buff Pass to the Dry Lake Aid Station where I found out that Dave James and Lizzy Hawker had just left. This was approximately 70 miles into what appeared to be a 110 mile race and I had a decision to make. Should I keep on running steady but remain conservative, or should I risk blowing up, push the pace, and see what would happen? I decided to hammer and found myself careening down the Spring Creek Trail back to the high school aid station at mile 75. I was running as hard as I could, pushing the envelope, and feeling amazing doing it. During this quick 5.5 mile stretch I passed both Dave and Lizzy and moved into 4th place overall, about 1 hour back from Timothy Olsen who was in command of third place. Karl and Dylan were out in front and I knew I was not going to catch those guys, but somehow I convinced myself that I could make up the 55 minutes on Tim over the final 35 miles of the race. I hammered up the Spring Creek Trail and back to Dry Lake as hard as I could. One wonderful thing about running in the dark is that you can look ridiculous and no one notices. I was frothing at the mouth, breathing hard, grunting, singing along to some of my tunes, and basically looking silly. No one knew though… and this brought a big smile to my face. Also keeping the positive vibes going was knowing that a good friend was sitting at home watching iRunFar's coverage of the race online throughout the night. I knew he must've been psyched to see me move up from 18th… to 15th… to 11th… to 9th… to 8th… to 6th, and now into 4th place overall.
I rolled into Dry Lake, grabbed some more food and coke and headed back up Buff Pass and back to Summit Lake Aid Station. I was running hard, hiking fast where I needed to, and generally holding it together. Just before reaching the top of the climb I began to feel a bit sleepy, and I could tell I was in need of that sunrise! At the Summit Lake Aid, I ate more wonderful mashed potatoes, washed it down with a bit of coke, and moved on. Here the course differed from the initial segment that we had been on. We took the Wyoming Trail from Summit Lake Aid back to Long Lake Aid which would add another two miles onto the day. I was slightly grumpy about having to do this… mostly because this all but guaranteed a 110+ mile race, but once I was actually on this buff double-track section I was stoked. The world was gathering light with the growing sunrise, my brain was rejuvenated with the calories, and I was stoked to be moving so fast this late in the game. At this point, cruising in 4th place, on the hunt for Timothy Olsen, and positive that my energy would last, I turned the tunes (Dave Matthews Band) up one more notch, put my head down and resolved to pulverize my legs the rest of the way.
Perhaps I lost focus, but after passing a few other folks on course (people running the Tortoise race which had started 5 hours earlier than the Hares race) I blazed through a 4-way intersection of trails and continued on what I thought was the 100 mile race course. The two people I had just passed (Jason Koop and his wife!) saw me go the wrong way and shouted for all it was worth to get me to turn around. Two problems though… I was running really fast through this section and was quite a distance down the trail already, and I was listening to my music with something approaching 'ear-damage-level' volume. Jason shouted exceptionally loud, but I never heard him and instead kept my head down while I cranked down the trail. I had pushed straight on to the 50 mile race course. (the 50 mile race was happening at the same time that morning!) The 100 mile course had been using a mix of pink and yellow markers, but the 50 mile course was only using the pink color markers. I failed to notice. Had I slowed down for just 1 second at the 4 way intersection and looked to my right, I would have seen the yellow markings for the 100 mile course and I would've been good as gold. Instead, I ended up 50 minutes down trail on the wrong course before coming to an aid station that was being set up. I asked: "Where am I?" and made the statement: "I'm really confused." The guys took one look at me and knew that I was in the 100 mile race. They felt so bad for me, offered me a coke, and pointed me back the several miles to the 100 mile course where I had made my error. Sure enough, after another 50 minutes. (50 minutes!) of running, I came across the intersection, went the proper direction, and within minutes was at the Long Lake Aid Station. (A 1 hour 40 minute detour!) (At least 8 miles, probably closer to 10 miles of extra running) (GPS died during this section)
I couldn't believe it. I was furious, yet remained composed. I had completely blown it and somehow I thought the only way to atone for it was to try to literally destroy my legs. I ran so hard back to the aid station, grabbed some food and hammered out of there. Every step was forceful, purposeful, and designed to get me back to the finish line. I was convinced I was in 10th place, out of the money, and a loser… but I kept hammering. I made it back to the top of Mt Werner and the final aid station before finally getting word that I had only lost 1 place on the overall leader board. (Lizzy Hawker passed me to take over 4th place overall) Still, I was livid… and ran the final 6.4 miles in about 45 minutes to finish in 5th place with a time of 22 hours and 36 minutes. (22:36). I was mad at myself for making a boneheaded error but that was somewhat tempered at the finish line knowing that despite running an extra 8 to 10 miles, I only lost 1 place and ended up fifth overall. The real 'tragedy' (if I can be dramatic for 1 second here) is that I don't have 'in-the-zone' efforts like this very often and to waste one by simply taking a wrong turn… well… that is a killer.
The overwhelming 'take-home' though? I ran 120 miles in 22 hours and 36 minutes and largely felt invincible for the last 80 miles. I guess I can't complain too much. I am deeply thankful for the solid effort, the great aid stations, the fantastic prize money, and the support from many people out on course. I am humbled by how much my wife supports my whacky endeavors, both on course and in life. Thanks babe. I am grateful for great friends like Dan and Becky who came out to watch, cheer, help out in many ways and show their support. Thanks you guys. I am grateful for strangers who help at the aid stations throughout the entire night! I am deeply appreciative of other strangers who help out by assisting at aid stations, watching our child, and showing support. (Thank you Annie Murphy (I think that's your last name)). As for Marty? He crushed it and finished in 6th place. Nice work Marty… thanks for the early-race chat. Congratulations to Karl for taking home the big victory and the hefty check. Same to Lizzy… nice work. Dylan Bowman and Timothy Olsen ran super strong as well. Nice job guys. Fantastic efforts by everyone who finished this tough race. Congrats all.
Here's to 'owning' our mistakes and moving forward to new challenges. Up next? The StumpJump 50km in Chattanooga, TN in just under 3 weeks. Really looking forward to racing in a state I've never been to. Keep on recovering. Live well. Train well. Focus well. DC


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It's not fair when the state suspends someone who's trying to do the right thing. My parents owned several small businesses. Growing up, they showed me now to navigate complicated bureaucracies. Now I love helping small business owners and profess...
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Barry Abbott concentrates his practice on financial services, general corporate, payments and e-commerce law. Barry has represented numerous international and domestic institutions and has extensive experience in corporate law and financial servic...
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cam and heads and valve spring mismatch.
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Originally Posted by
3000 rpms up the driveway? Why would you do that?
If you drive normal you cannot even tell its there. A 3000 rated will only footbrake test till 2200. Less in a 305. Dynamic converters make custom stalls. The one they built me is perfect. It even uses the GNX core, for that grand national type acceleration. I cant think of any driving condition it would negatively impact. Towing, I don't know? I towed 7500+ lbs on a wt distribution hitch. The trans was fine. At 65 Im at 2800rpm. Not terrible.
Every car today has at least 2400-2600 stall.
Again It will not even be noticeable. Until you use it. Consider it part of the basic muscle car package.
im so green behind the ears that the torque converter thing you just explained i had no clue of those facts. did not realize that. i thought converters only force the car to move after a certain fluid volume is reached to make a certain pressure to spin the pump. thank you for the advice it unlocks yet another peice to the puzzle that i had absolutely no clue about. i once had a triple cylider snowmobile that had stiff spring and light weights in the centrifigal clutch. trying to pull up to a gas pump with it could potentially rip your arms off. thought the high stall converters had the same affect. just common physics sense. im good a billiards hahaha. could use some info on valve springs and camshaft useable dates.
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Kronos for Energy provides tools that help utilities companies manage their most flexible and strategic asset - the workforce. Utilities can gain real-time visibility into labor performance and costs to improve operational decision making, and allocate the right labor resources based on demand to help keep project and work schedules on track and on budget. At the same time, Kronos solutions help support compliance with federal and organizational labor policies and employee safety requirements such as OSHA and fatigue management programs. Kronos for Energy can also help utilities companies:
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Kate Upton: From the Daytona 500 to the Oscar Parties
Posted Monday February 27, 2012 4:10 PM GMT
Stepping out for a promo appearance, Kate Upton was spotted at the Daytona 500 in Daytona, Florida on Sunday (February 26).
The sexy supermodel protected herself from the rain with the help of an umbrella while there to serve as the grand marshal of the race - which ended up being rained out.
During the appearance, Upton met up with Jane Lynch, Sean Hayes, Chris Diamantopoulos and Will Sasso to promote their upcoming film "The Three Stooges."
Shortly after her gig at the speedway, Ms Upton hopped a plane to Los Angeles, CA just in time to attend the Vanity Fair Oscar Party.
Enjoy the pictures of Kate Upton at the Daytona 500 and the Vanity Fair party (February 26).
Photo Credit: Getty Images, Wenn.com


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AAL is indeed a band of high caliber
Life, and when I stumbled across this band i almost don't believe it was true. This is the best band EVER!! I am a drummer and I am amazed over and over gain by the talent of every member in this band! They r a very unique band, the only other band that is like them that I know of is Plini. They r good too but AAL is THE BEST! Awesome band! Keep making great music!
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IKEA seeks to drive food sales growth with eco-friendly menu
By Anna Ringstrom
STOCKHOLM (Reuters) - Swedish furniture retailer IKEA said sales growth at its food business could accelerate in coming years, helped by a drive to meet customer demand for healthy and eco-friendly dishes.
IKEA Food Services, which runs the group's in-store budget restaurants and Swedish food stores, in January reported an 8.6 percent rise in sales in its 2013/14 fiscal year, to 1.7 billion euros ($1.8 billion).
The division's boss, Michael La Cour, said it could outstrip that sales growth in coming years as it catered better for customers who had become more conscious about the nutritional value and sourcing of their food in recent years.
"This massive shift in terms of how to serve and what people expect around food of course has an impact on how we want to do food going forward at IKEA," he told Reuters on Wednesday.
"The food business will be a strong contributor to IKEA. We don't think the 8.6 percent is the ceiling."
The food business accounted for 5 percent of sales in the fiscal year to August 2014 at IKEA Group, which owns IKEA Food Services and most of the IKEA stores worldwide. La Cour said he expected that proportion to be at least unchanged this year.
In a first step of its food drive, IKEA on Wednesday launched a vegan version of its signature Swedish meatballs with a carbon footprint 30 times less than that of the meat-based product. Within months, all seafood on sale will be certified as sustainably fished, La Cour added.
La Cour said the 2013 horse meat scandal - which saw IKEA withdraw its meatballs across most of Europe after a supplier found a batch that contained traces of horse meat - was a wake-up call for the food industry. Continued...


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zebra - is those the ones you have benn waiting on? Glad you got em almost like christmas aint it! lol
psys - good sized vinyls with sick hand drawns thanks
zebra - is those the ones you have benn waiting on? Glad you got em almost like christmas aint it! lol
psys - good sized vinyls with sick hand drawns thanks
CHOTCH - got some sweet ass multi colored vinyls with hot chick on it hooked it up fat 20+ and also handdrawns - she kicks azz
Crevice Creep Pack - sick full page sticker, a couple hand drawns, a couple vinyls, printed paper and 4 pins love the variety
pinak pack - sick collabos, great handrawn stickeys
Sending a few out to BOMIT. I wonder if I will ever get some back from them when I order packs from em. Haha.
you really getting some ups there zebra good shit
@zebra nice thanks for the pics man, i got your vinyls going out tonight ill hit you up with some pics. Anyone on here using instagram? look me up @wearezombies_fys
Look who I found....
I have been out of paint markers for the longest time and just found out wal mart has painters so i swiped some.and wizard sorry but your pack hasnt gone out yet im broke and like i said its kinda heavy so it will cost a little to get ti ireland but dont worry you will get it
i put a bunch of shit up in sac but didnt have my camera
Hey guys sorry i've been inactive lately coz I got sick and focused my shit on videogames for a few weeks but im back now and i'll be sending out packs asap
And I did also just receive two badass packs from PSYS and Tbone so i'll have pics of those up soon as well
@Zebra good to see you finally got my pack!!
haha made this one for my cousin


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Maple Grove Homes and Real Estate for Sale
Maple Grove homes recently listed on the Northstar IDX/MLS system:
Display ALL Maple Grove real estate by clicking here! Check out idx data on almost any home for sale right now on the Maple Grove MN MLS!
Maple Grove is a booming third tier community with an eclectic mix of residential real estate opportunities, and is host to a multitude of thriving businesses, entertainment facilities, retailers, restaurants, recreation, and opportunities.
The only people who lived in the area were the Winnebago Indians until 1851, when the area was settled by Louis Grvais. Soon farms, churches, and newly constructed homes dotted the landscape. Schoolhouses started to replace the vacant homes that were first used for schools. The economy centered around potatoes, creameries, and lumber mills. By 1915, the Great Northern train depot was in full swing, and a cheese factory was constructed.
After World War Two, the population of Maple Grove MN started to boom. The 1880 population was only 1155. By 2000, the population exceeded 54000.
Today, Maple Grove is an economic powerhouse, and is one of the fastest growing communities in the area. Suprisingly, city services are keeping pace with residential and commercial growth. Land use plans have been implemented to ensure that growth will continue without compromising the natural beauty of the area. Be sure to visit Arbor Lakes area the next time you are shopping. Check out the Arbor Lakes boutiques and eateries, upscale shops, and the Arbor Lakes 16-screen theater.
Homes for Sale in Maple Grove Minnesota.
Generally there are about 350-400 single family homes available on the Maple Grove MLS. It is still possible to find some fixer upper split entry homes that were built ihn the 70's and early '80's for under 200k, but they will need a little elbow grease. Many people who are moving to the northwest suburbs will choose Maple Grove over Plymouth and other suburbs because the homes are so much more affordable. But with the tremendous growth occuring along the I94 corridor to St. Cloud, these opportunities won't last forever.
Luxury Maple Grove Real Estate
On the luxury end, Dunlavin Woods contains some 1.4M+ six plus bedroom homes on the north side of Weaver Lake that boast over 6000 square feet, built by Andreen Construction. If you have never seen walnut hardwood floors before (I haven't) then you should check this out. Alder cabinets are also a unique and unsual upgrade. Other upscale builders include Lattrell Builders, Timberwood Homes, K. Hovnanian, Creek Hill Custom Homes, Quality Checked Construction Inc, Hedburg Homes, and U.S. Home Corporation. Explore the newer neighborhoods of White Oak Acres, Kilarney Glen, Maple Trace, Hidden Creek Estates, and Shannon Lough. Whatever your tastes and requirements are, you will be able to satisfy them in the Maple Grove real estate market.
Townhomes in Maple Grove MN
Townhomes in Maple Grove generally cost a little less than they do in Plymouth and some of the other third tier suburbs, but the location along the Minneapolis-St. Cloud development corridor along Interstate 94 could portend great market potential in the future. Even now, the proximity to 94, 494, and 694 means that many townhome buyers will purchase in the Maple Grove area because of the easy commute to Minneapolis and downtown St. Paul. Quad homes close to Weaver Lake Road offer affordability, huge lawn areas, decks, fireplaces, L-shaped family rooms, trees, and walkouts. Many of these have a large master suite, a large walk-in closet, and a large walk-thru master bath with a 3/4 bath in the basement, frequently with a third bedroom on the lower level, or at least the potential for one. Glen Eagle condos off of 169 on Magda are also available at starter-level prices, but if you are thinking of buying one of these for investments, it is possible that rentals are not allowed, so you might want to check that out first, In any case, you can have a ten day right of recission to review all townhome documents to check out rules on renters, pets, financial status of the association, or other pertinent issues.
Cranberry Meadow Courthomes are some lovely two-story units, with vaults, eat-in kitchen with penninsula, gas fireplaces, second floor laundry, about 1300 square feet, and are conveniently located close to shopping, transportation, and parks. Cedar Ponds two story side by side units are roomy, and have lofts overlooking the living room. Rottlund and Town&Country two story units dominate much of the market in Maple Grove.
Free Homebuyer or Homeseller Consultation
If you are considering buying or selling a Maple Grove townhome please call me for a free consultation at 612-296-0007. Avoid high commissions of the national franchise companies and related "administrative" overhead. Call today at 612-296-0007 for a free quote and consultation. Your Maple Grove property. sale will benefit from aggressive traditional and electronic advertising to bring you the most exposure to the largest number of qualified buyers possible. I avoid dual agency and seek to represent your best interests in the transaction. Ask for a free market analysis today!
Maple Grove Lake Homes
Although lakeshore in this area is in very high demand, the Maple Grove MLS will usually offer a handful of lakefront properies on Weaver Lake, Fish Lake, Eagle Lake, and Rice Lake. Use the Minnesota Lake Home Finder to explore lakefront in Maple Grove and other Minnesota communities.
Maple Grove MN Public Schools
School District 279
No-pressure help from a Maple Grove MN REALTOR
Send us a call at 612-296-0007 if you have questions or need a little help finding the ideal property. We are here to help. All inquiries are confidential! This area is an integral part of my service area, and I am committed to ensuring that you get the best value and a real estate transaction that is favorable to your terms.
See Maple Grove luxury homes and upscale real estate listings.


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Fracking activists are taking part in a 'day of action' today spanning Balcombe, London and Lichfield. They have blocaded the Cuadrilla drilling site in Sussex (leading to the arrest of Green Party MP Caroline Lucas), chained themselves to the headquarters of Bell Pottinger (Cuadrilla's PR company) in central London and for good measure, also occupied Bell Pottinger's Lichfield office. Meanwhile about 20 people spent the morning protesting outside the constituency office of Tory MP for Balcombe, Francis Maude.
This follows a busy weekend for the anti-frackers. Balcombe in Sussex was once again the centre of public attention, with Reclaim the Power set up camp there on Friday, adding strength to local and national outcry over fracking in the UK.
The camp runs until tomorrow (Tuesday), and includes workshops on political action and sustainable living. But its focus is empowering ordinary people to take direct action against the corporate energy sector, which is driving fuel poverty and climate change.
Hundreds of visitors converged in Balcombe from community anti-fracking groups across the country: No Fracking in Balcombe Society and Residents Action on Fylde Fracking - two of the groups most in the firing line at the moment- made links with environmental activists from groups like No Dash for Gas and Fuel Poverty Action.
Yesterday, a march of 2,000 people united local and regional people who object to fracking with the "protestors'" camp which has been by the drill site gate for the last three weeks.
Local residents and politicians spoke against oil and gas exploration in their area, and crucially condemned fracking anywhere in the UK. Several hundred people peacefully defied police lines to put a human ring around the drill site.
To be continued....


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When the nation's only black senator isn't offered a speaking spot at the 50th anniversary of Dr. Martin Luther King Jr.'s "I Have a Dream Speech," something is wrong with politics in America. To mark the March on Washington on August 28, not a single Republican was offered a speaking spot at the event, and if you're Salon.com, the nation's sole black senator did not deserve a speaking spot either.
While there are conflicting reports as to whether Tim Scott (R-S.C.) was denied a speaking role at the event because he was unable to attend, Salon takes it a step further by suggesting his conservative values deem him unworthy of a speaking role.
Salon's Alex Seitz-Wald ridiculously argues that because Scott is a Republican he is "pretty clearly at odds with that of the modern civil rights movement." What Seitz-Wald means is that Scott does not support the Democratic Party's definition of "civil rights."
Among Salon's criticism of Senator Scott is his support for traditional marriage, pro-life values, and worst of all, "supported the Supreme Court's decision to essentially throw out the Voting Rights Act." Senator Scott supported the court's ruling by saying that "punishing six Southern states because of past failures does not help us in the present and certainly does not help find our path to the future."
In addition, gay marriage and abortion had nothing to do with the original civil rights movement, but instead are simply part of the Democratic Party platform. Seitz-Wald never explains why valuing life, especially wanting to protect innocent black babies makes him the antithesis of the modern civil rights movement.
Black teenage girls are 4x as likely to have an abortion as white girls, and Scott's pro-life record seeks to protect all babies, especially black babies. In reality, Seitz-Wald seems more upset that Scott doesn't support the Democratic Party's pro-abortion stance than Senator Scott's insistence that all babies be given a chance to live.
Hilariously, Seitz-Wald notes that Scott's "F" rating from the NAACP is even more evidence that the South Carolina Republican is against modern civil rights.
For those of you curious, one of the reasons the NAACP gave Senator Scott an "F" was because he does not support EPA efforts to regulate greenhouse gas emissions and the implementation of ObamaCare.
So now climate change and ObamaCare are civil rights issues? No, these are liberal issues that the left has deemed "civil rights" issues, and opposition to the left's agenda means you are against civil rights.
In short, Salon has disgustingly distorted "civil rights" with liberal issues and has smeared Republicans who oppose the left's agenda as being anti-civil rights.
Given that the 50th anniversary of MLK's speech was more of a Democratic Party rally than an actual civil rights event, Scott's conservative message probably would have been poorly received by the liberal crowd. Despite this, to accuse the nation's sole black senator of being against civil rights because he doesn't support the liberal agenda is shameful and defeats the true goals of Dr. King's speech 50 years ago.


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The 20th anniversary of World Press Freedom Day swept past on Friday. It was celebrated under the theme "Safe to Speak: Securing Freedom of Expression in All Media".
According to the United Nations Educational, Scientific, and Cultural Organisation (Unesco), the world's chief authority on matters of freedom of expression, the theme aimed to draw attention to "safety of journalists, the issue of impunity, and online safety".
The inclusion of online safety is key for us in Uganda. About two years ago, police arrested and charged Mr Timothy Kalyegira with criminal libel for writing critically of the government in his online publication – The Uganda Record.
Around the same time, the telecoms regulator, Uganda Communications Commission, asked Internet service providers to block Twitter and Facebook for at least 24 hours.
There had been attempts as well to get mobile phone companies to block text messages with toxic words such as "revolution".
The government made these moves during moments of heightened tension in the country: towards the end of campaigns for the 2011 general elections, and during the walk-to-work protests against the rising cost of living that erupted in the aftermath of voting.
The government was jittery, eager to stop anything that could potentially snowball into the Arab Spring-style movement then felling kleptocrat after kleptocrat in North Africa.
The Broadcasting Council barred TV stations from relaying live images of the protests because security agents were beating up protesters and journalists on Kampala streets.
The physical harassment of journalists is still prevalent. Prison warders recently clobbered journalists covering the aftermath of a jailbreak in Kalisizo.
The government's harassment of writers online seems to have gone with the end of the active phase of the walk-to-work protests.
That is not to say no one within the "system" is watching the online goings-on.
Free speech is so important that to gag people is a commentary on the quality of a particular government than on anything else. Secure, responsive and accountable governments go only after hate speech; they do not bother with their critics. When they do, they argue their case. They do not intimidate anyone into toeing the line – straight or zigzag.
Unesco says, appropriately, that World Press Freedom Day "serves as a reminder to governments of the need to respect their commitment to press freedom". They should.
Countries such as Uganda have signed various international treaties, conventions and protocols committing to the unfettered exercise of freedom of speech by their citizens.
They have enshrined these values in their national constitutions. A small annual reminder does not hurt all the same.
But Unesco has another message as well regarding the day. May 3 is "also a day of reflection among media professionals about issues of press freedom and professional ethics".
That is a challenge to media owners and practitioners. Media owners need to stop and think about whether their outlets promote free expression and serve the public interest or they are entirely about making money.
In the long term, even medium term, a country whose citizens freely express themselves becomes more tolerant, more democratic and more stable. That is good for business.
- Monitor offices under police siege
- State should stop muzzling free press – Monitor Editors
- Matembe condemns police siege on Monitor
- Monitor Publications under Police Siege
- MPs want Shs129b for health workers' salary
- Game meat consumption a threat to animal population – report
- Two held over pinning posters
- Billions in tourism tax set to boost the sector
- Bidandi taken to intensive care unit after accident
- Museveni approves thumbprint use in 2016
- Monitor Publications under Police Siege
- This Muhoozi thing, is it a project or projection?
- Two held over pinning posters
- Lukwago silent on plans by councillors to unseat him
- Bidandi taken to intensive care unit after accident
- How she maintains her identity without the "Barack's sister" tag
- Ochola lives marriage dream 25 years later
- He survived death
- Attempt to prohibit miniskirts further exposes NRM regime


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LED Lenser P5.2 LED Flashlight - 140 Lumens - Uses 1 x AA Battery
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LED Lenser .2 ModelsLED Lenser has updated its famously reliable flashlights with incredible new technologies. Their professional P series and tactical T series flashlights have gotten hear-to-tail makeovers to increase their power, ergonomics, and styling. For starters, new LED's and optics in the .2 models have significantly boosted outputs. This means that the same quality-constructed LED Lenser models that have been relied upon by men and women in uniform all over the world now give you a brighter, more refined beam and longer throw distances. A new switch has also been developed. The larger format allows for more surface area so that all of the light's modes can be accessed rapidly with one hand. A flatter design also allows for tail-standing for hands-free area lighting. A new matte-black finish gives a grippier feel, meaning these lights are comfortable to use over long periods, in adverse conditions, and when wearing bulky gloves.
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In 2007, the Centre for Indo Canadian Studies came together in partnership with the Khalsa Diwan Society, Abbotsford and the National Historic Site of Canada Gur Sikh Temple to build the www.canadiansikhheritage.ca website. The faculty and staff at the Centre for Indo-Canadian Studies played a pivotal role in the research aspect of this website project, providing the historical content for the website, as well as engaging in over five years of pioneer research including: interviewing, transcribing and narrating the stories of the Indo-Canadian pioneers who helped to build our country. These pioneer interviews are still on going today.
For the pioneer work the CICS has undertaken, please visit www.canadiansikhheritage.ca.
The funding for this project was generously provided by the Canadian Culture Online, Government of Canada.
Through the support of Canadian Culture Online, Government of Canada, the Khalsa Diwan Society, Abbotsford, the Canadian Sikh Heritage Research Team, WebSix: the web developers, as well as supporters from the local community and the University of the Fraser Valley's Centre for Indo Canadian Studies, this website has brought Canada's unique history to the forefront.
As you explore the www.canadiansikhheritage.ca website, there are a number of detailed sections, some of which will provide you with an understanding of the history of the first Sikh pioneers to migrate to British Columbia, their amazing life stories, experiences, and accomplishments; and finally, the major role which the Khalsa Diwan Society and the Gur Sikh Temple has played in such an intrinsic and colourful history.
The focus of the website centers around the establishment of the Gur Sikh Temple in Abbotsford as a National Heritage Site of Canada. However, in order to understand the full context of British Columbia's past, please do explore the sections explaining the history of Sikhism and the Passage to Canada, which includes interviews with Sikh pioneers and images from their past.
We hope that in exploring the many aspects of this website, that you, the audience, will gain a live, visual, and historical understanding on the 100 year history of Sikh migration to British Columbia and the significance of such a history to the historical framework of Canadian history.


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April 11, 2001
MURFREESBORO, Tenn. - Middle Tennessee swept a double header from UAB on Wednesday, setting a school record in the process as pitchers Jennifer Martinez and Stayc Preator combined to extend their streak of scoreless innings to 39 straight.
Martinez threw a six-inning shutout in game one of the double-header, increasing her personal scoreless innings streak to 20 as the Lady Raiders won 8-0.
The Lady Raiders posted four runs in the first two innings. Martinez allowed only one runner to reach third base. She struck out seven in the six-inning one-hit shutout.
Kelli Thompson (8-14) started game one for UAB, allowing four complete innings, allowing five runs on six hits, with five walks and three strikeouts. Mandie Wyatt worked the final inning for the Blazers, allowing two runs on three hits.
Center fielder Cliffanie Engram collected the only UAB hit of game one with a single up the middle off Martinez in the top of the first inning.
Middle Tennessee scored in the first inning of game two when Kip Phillips led off with a single and later scored on a passed ball.
Preator held UAB scoreless through five innings before two errors on the same play allowed the Blazers their only run of the day. Amanda Ellis pinch hit for Emily Scarbrough, reached on an error by center fielder Phillips, advanced to third and scored on an error by shortstop Steffi Silva.
UAB starter Kerri Foster held the Lady Raiders scoreless until the bottom of the eighth when Jennifer Martinezhit a sacrifice fly to center field scoring Lisa Sherman for a 2-1 win.
Foster (9-10) worked seven and two-thirds innings, allowing two runs (both unearned) on six hits, with one walk and 12 strikeouts.
The Lady Raiders, who entered Wednesday's games with the nation's seventh-longest current winning streak, has now won six straight to improve to 27-14 overall.
UAB continues its road trip Friday and Saturday with a three-game C-USA series at Saint Louis, and returns home to host Florida State in a double-header Sunday at George Ward Park, beginning at 1:00 pm.


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Two of Titans' most potent offensive playmakers, wide receiver Kenny Britt and tight end Jared Cook , sustained minor injuries during Tennessee's 44-41 overtime win against the Detroit Lions. Britt told The Tennesseean he has a "slightly sprained ankle" while Cook hurt the AC joint in his left shoulder. Both players are believed to be healthy enough to play in Week 4.
Fantasy Analysis:
Britt seems to be one of those players who will very rarely ever be 100-percent. Cook had his arm in a sling and was unable to return in the second half, but head coach Mike Munchak thinks he'll be ready. Britt had 11 targets though, which bodes well for his fantasy future and Cook had four before leaving the game. Given the high number of breakout players at the tight end position, dropping Cook isn't out of the question. He only ranks 20th in the NFL in targets (16). Although some may disagree, Britt is worth a speculative add since, when healthy, he can be Tennessee's most-productive receiver.


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Paul Ryan is a Liar Just Like Mitt
August 15, 2012 at 6:26 PM Leave a comment
Whiplash anyone?
Speaking at his alma mater Miami University in Ohio, Paul Ryan slammed President Obama for adopting Medicare cuts that, until last week, were openly supported by Ryan himself.
More here.
Pardon my language but Romney and Ryan will say Any. Fucking. Thing.
Entry filed under: 2012 Election, Republicans, Including Wingers & "Moderates".
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Welcome! To help a mesothelioma patient quickly, easily maximize the chance for long term survival and good quality of life, we have uniquely organized a group of mesothelioma doctors, researchers, survivors and caregivers to develop this information guide. Fight smart!
    If you are familiar with mesothelioma issues, just find your topic menu to the left and below to continue.
    If you are unfamiliar or newly diagnosed, like most people you must be under stress, hard to think well. Here is what we would do to find the best mesothelioma treatment. First, from our guide find the best mesothelioma doctor in the US or near you. If your doctor tells you will be largely cured, then your quest for treatment has succeeded.
    If you have treatment but that alone is not a complete cure, or the stage is too advanced for standard treatment, then a mesothelioma clinical trial may offer best hope for cure.
    A good mesothelioma support group could be of critical importance for your quality of life. They can provide emotional, spiritual support, and reduce your fear, anxiety.
    To further maximize your chance to beat mesothelioma, go to mesothelioma survivor tips, and follow the news for better mesothelioma treatment.
Mesothelioma Information, Treatment:    Understanding mesothelioma is basic for pro-active care and the best treatment. Guide to easy learning with different levels of mesothelioma information, visual/audio tutorial; to find the best standard mesothelioma treatment available; to find more questions-answers or related information in the resource.
What to Do:    To do list when you are diagnosed with mesothelioma, prepared by experienced cancer experts and survivors.
Find Best Doctor, Hospital:    Finding the best doctor(s) can be crucial for successful mesothelioma treatment. Section covers to find the best mesothelioma doctor in the US by checking the US Cancer Hospital Ranking; to find one near you by searching for an NCI designated cancer center (elite cancer hospital) on the map near you; to ask a doctor online to make sure; how to check on a doctor, hospital; how to ask different questions for different visits to your mesothelioma doctor.
Mesothelioma Side Effect Coping, Treatment:    Mesothelioma treatment side effect coping tips, information; home remedy; ask experts
Mesothelioma Clinical Trial:    Clinical trials may increase your chance for cure, or if mesothelioma advanced they hold the best hope for treatment. However it is a real challenge to locate your best trial. Some of the doctors may provide help. There are also fee-based agency to help you search, but their service quality may vary.
Mesothelioma Financial, Insurance, Legal Assistance, Support:    Mesothelioma financial, insurance, legal information, assistance; medication, drug, travel, transportation, lodging help; self-search, or help find.
Mesothelioma Alternative Treatment:    One should be open minded to alternative treatment. Guide to how to find reliable mesothelioma alternative treatment; recommended treatment; find your local alternative treatment doctors; ask expert questions.
Mesothelioma Support:    Mesothelioma support information; local resources; collection of chat rooms, message board, mailing lists; mesothelioma survivor story; where to find face-to-face support, phone peer support; cancer hotline to talk to a trained personnel.
Mesothelioma Survivor Tips:    How to manage day to day life, care; how to beat mesothelioma tips from advanced stage survivor; good diet and nutrition, how to exercise.
Caregiver, Family:    Guide to cancer caregiver information, support; message board for cancer caregivers.
News, Newsletter:    Mesothelioma news summary, research publication, breaking news from various conferences; newsletters from numerous top organizations.
Advocacy:    Know and advocate for your patient rights, quality treatment.
Mesothelioma Book, Publication:    Popular mesothelioma book collection; also, general cancer issues, how to cope with cancer, mind/body support book, alternative treatment, nutrition, caregiver, end of life/ grief issues book.
Cancer Hospice, Home Care Guide:    Information about cancer hospice, home care; practical tips.


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(Other) Stuff to Do While You Look for Work… You Lucky Dog
When I was on unemployment, I was really happy. Don't get me wrong, I love having my own business but getting paid to meet people, learn new things, think about my potential, help people and so on was great.
If you are unemployed, do you see yourself as lucky? Are you having fun? Are you showing your children how to thrive in times of change? (Believe me, whatever changes you have seen in your life will be child's play compared to what they will go through.)
I found this terrific article written by Tim Tyrell-Smith titled, "101 (Other) Things You Can Do While Looking for a Job." Here are a few of Tim's suggestions
- Pick one person in your network and find them a job
- Put on an elaborate puppet show for your kids (fun, colorful socks work great)
- Join (or start) a book club and actually read the assigned books
- Write a thank you note to an inspirational high school or college teacher
You may or may be surprised at all the "yes buts" I hear everyday. "I would join a book club but when I go back to work I won't have time." " Why should I help someone else, I need help!)" blah, blah, blah. Just do it, you lucky, lucky dog.
2 Comments
By Tim Tyrell-Smith, September 12, 2011 @ 9:44 am
Hey Deborah – Thanks for sharing that post with your readers. So glad when I hear someone is taking advantage of the free time available during a transition. But not everyone does . . .
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I applied online. I interviewed at PepsiCo (Salinas, CA) in May 2017.
The phone screen process is the dumbest thing I've ever had to do. You answer a series of questions on a recorded line. The questions aren't something you can prepare for. I would much rather talk to a real person rather than leave a 10 min voicemail answering questions. It's unrealistic and there's no point to it. It's MUCH more than just a "phone screen" it's a real interview with a voicemail machine. I guess we'll see how I did in a few days
Interview Questions
I applied online. I interviewed at PepsiCo in April 2017.
Phone interview was about 45 min and very informative and they seem to be very honest about the job and what to expect. Had about 6 questions that they had asked.
Interview Questions
I applied through an employee referral. The process took 2 weeks. I interviewed at PepsiCo (Bronson, MI) in December 2016.
Got 3 phones calls explains the process
N when I got there there were other people waiting for the same job, n asked the typical tell me about a situation, which I failed at!
Interview Questions
I applied online. The process took 3 weeks. I interviewed at PepsiCo (Tampa, FL) in October 2016.
Timely online test - be prepared to be exhausted by the amount of questions thrown at you, informational meeting to describe position and salary which is used apparently to weed out applicants by information overload and promises of overworking and no home life, same day first interview to be offered to route ride and work with a driver to rotate his stress dated product due to overselling accounts to make plan and then not to get paid for the whole day, second interview scheduled if interested. offer of job.What was I thinking...that's right I turned it down. 3 a.m. start times and staggered days off. I should be in management there to reorganize the system and advocate for the guys working for frito-lay's profit margin and budget. They forgot about work life balance for them
Interview Questions
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I applied through a recruiter. The process took 3 days. I interviewed at PepsiCo (Dallas, TX) in August 2016.
I was contacted via phone call after setting it up via their website and signing up for a specific time. On the call I was asked a series of questions associated with the job and if i would be able to complete certain responsibilities. It only took about 5 minutes and the questions were mostly focused around the job responsibilities and if you are able to do them.
Interview Questions
Helpful (1)
I applied in-person. The process took 1 day. I interviewed at PepsiCo (Aston, PA).
You were interviewed the day you applied for the job by all 5 District Managers who worked at that particular plant and then you were notified later if you would be getting a second interview. At that point you can pretty much tell if you have a chance or not. The Managers are very down to earth people most appear to be just out of college. Everyone at Frito Lay must start at the bottom and learn the basics of Route Sales and delivery.
Interview Questions
I applied online. The process took 2+ months. I interviewed at PepsiCo (Lorton, VA) in June 2016.
Let me start by saying that this is a long process. From the moment I applied online it took a little over 2 months for me to actually start. It started as an online application, then a scheduled phone interview which was essentially reviewing your resume and relating it to the job you are applying for, then there was an in person interview with the sales coordinator and market leader. My interview was 1:1 but I've read that others have had group panel interviews. I was offered a position and sent for a DOT physical and urine screening (others reported hair follicle test). Each interview was roughly 20 minutes and was very professional and straight forward. There were roughly 6-7 simple questions in all and a good part of both interviews were spent trying to talk you out of the job (early mornings, lots of bending and lifting etc.) because the training seems lengthy and expensive for someone who isn't going to work out.
I did make an observation that most RSRs at this location appear to be between 24-35, male, and in fairly decent shape.
Interview Questions
Non negotiable flat rate weekly salary during the first 6-9 months then full commisioned
I applied online. The process took 1+ week. I interviewed at PepsiCo (Sylmar, CA) in June 2016.
First you take an online test. Then you're emailed an invitation to an info session which is basically a waste of time. The information you're given can be described to you in the interview. Why even have this step, especially since most people have jobs and need to take time off work to attend this unnecessary session. You're then given a phone interview which is very easy; you're basically asked if the pay and benefits are ok with you. Then you schedule a panel interview that's very impersonal; they read off a list of questions. I really don't like this type of interview because it really gives you the sense that the people who run this business are just robots with can't think for themselves that they have to read off a script. Then, if you pass the interview, they call you to schedule a ride along with an experienced rep. If you don't pass, they email you. Unnecessarily long process.
Interview Questions
Helpful (1)
I applied online. The process took 3 weeks. I interviewed at PepsiCo (Augusta, GA) in May 2016.
For me to actually get an interview, it really took a long time. But once they started the process it was pretty smooth. First phase was more like an informational type session with 10 or more people. Afterwards we split up into individual interviews. They don't specify but please wear a suit. Stand out and also be yourself. I interviewed with three managers that day and got my ride along scheduled as well. The ride along was great as well. The rep was very nice and informative. We started at 3 and got done about 1. During this phase please help out and ASK TONS OF QUESTIONS! When we got back the manager asked about my experience and I informed him that I was ready to move forward. Background was cleared, drug test was cleared, and now my start date is less than 3 days away. Great overall experience. Just make sure your job history is accurate for DOT.
Interview Questions
Helpful (1)
I applied online. The process took 4 weeks. I interviewed at PepsiCo (Sacramento, CA) in April 2016.
Interview took 5 hours total time. Not all 5 hours you are being interviewed. It felt like bulk interview. All the candidates are in a room and they give us a presentation on the company then people asked questions and then they took you in a few at a time to do a separate one on one interview or a panel interview. If you pass the first round (panel or one on one) you get to do the second interview which is opposite of the first round...aka if you had panel the first round you will have the one on one the second round.
Interview Questions
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Kobo Unveils New Family of Ebook Readers
Posted on September 11, 2012
Kobo has announced a new family of ereaders and tablets. They include the Kobo Glo, Kobo Mini, Kobo Touch and the Kobo Arc.
The Kobo Glo features a 6-inch E Ink screen and adjustable ComfortLight technology. It is available in black, white, pink, blue and silver for $129.99 starting October 1st.
The Kobo Mini is a 5-inch E Ink reader. It is available in black or white and offers a selection of three Kobo SnapBacks in teal, ruby red and purple. The device is also available starting October 1st for $79.99.
The Kobo Touch is a touchscreen ereader that retails for $99.99. It is available in seven different languages.
The Kobo Arc is a new Android 4.0 tablet with a 7-inch display. It features a 1.3 megapixel camera, built-in microphone and speakers with SRS TruMedia. It is available in black and white with interchangeable blue and purple SnapBacks. The Kobo Arc will cost $199.99 for a 8 GB device and $249.99 for a 16 GB. It will be available starting in November.
Kobo readers can be found at Bestbuy.com in the U.S. and Indigo, Future Ship and Best Buy in Cananda.
Photo: Kobo
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