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Article Preview
'TOUCH WOOD' SOLD TO PRODUCER HERE; Alfred de Liagre Jr. Expects to Present Dodie Smith's Play on Broadway Next Fall RECINALD DENNY TO LEAD 'No Code to Guide Her' Is Off for This Season--Carroll to Sail for Home Next Week
Dodie Smith's "Touch Wood," which reportedly has been sold at least five times for production in this country but always was abandoned because of the author's stipulations regarding the casting, seems destined to reach Broadway next Fall, with Reginald Denny in the lead.
February 16, 1940
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Rubondo Island might just be the most unique destination in Africa for those seeking exceptional adventure experiences in the wilderness. This 25 000 hectare paradise in the south-western corner of the expansive Lake Victoria is the largest island national park on the continent and is all but untouched by man. Elephants roam wild, families of chimpanzees cascade through the boughs of pristine indigenous forest, and the waters teem with the iconic Nile Perch. If you're a seasoned adventure traveller or safari goer who has seen it all, just wait until you lay eyes on Rubondo Island Camp with its unrivalled access to this one-of-a-kind destination. This small and exclusive camp offers forest walks, game-watching, birding excursions, canoe trips, trekking through wild chimpanzee territory, catch-and-release fishing and a host of other eco-sensitive activities. This property was named one of National Geographic's Most Unique Lodges in the World.
This small and exclusive camp of just eight luxury cottages – all built by hand, using traditional skills and local materials to ensure a light footprint on the island's delicate eco-system – offers unrivalled access to this one-of-a-kind destination, with forest walks, game-watching, birding excursions, canoe trips, trekking through wild chimpanzee territory, catch-and-release fishing and a host of other eco-sensitive activities. The luxury cottages are powered by generator, and are spaced 10 to 20 metres apart to ensure tranquil seclusion for each guest. Moonlight plays on the waters of the Lake as you drift off to sleep. Get some rest; the eagles will be rousing you soon enough.
See Above Description.
As birds sing their roosting lullabies, guests gather in the stylish dining area for a taste of Africa and to swop tales from the day. With Henk, the camp's manager and professional sports fisher by your side, you also have the option to go fishing on Lake Victoria; the fish you catch can be deliciously prepared to perfection by the camp's chef.
Daily boat excursions and game drives in open vehicles are conducted by Asilia's expert wilderness guides.
Explore the habitat of Rubondo's wild chimpanzees on foot and learn about these remarkable primates. Although the island's chimps are slowly habituating to humans, sightings unfortunately cannot be guaranteed. As these are wild chimps the emphasis of this experience is more on learning about chimps and the habituation process. The camp does have a small chimp research library that contains photos of the chimps and details of the habituation process. Members of the research team are often in camp and are likely to join guests for drinks or dinner.
Rubondo Island National Park is a twitchers paradise with over 300 endemic and migratory species. December to February is best for spotting migratory birds. This fully protected Island National Park provides exceptional habitat variety ensuring there is always something new to experience or record; open grasslands on rocky ridges, giant old forest tree stands, dense woodland thickets, expanses of lakeshore papyrus and small breeding islands all help with the species count. Add in that large-lake evening light, the aqua acoustics and the drama of the skies and you have a sensuous recipe for any nature lover or photographer.
Guided walks through the forest are tailored to each guest, and can stretch from an hour to half a day.
Rubondo Island offers some of the best fishing in East Africa and is renowned worldwide amongst anglers who travel here for the opportunity to hook into one of Africa's biggest and most sought after fresh water species – the Nile Perch, which can weight in excess of 100kg's. Tilapia are an entertaining target for recreational fishermen Catch-and-release fishing from Rubondo Island Camp can be done off the beach and rocks, or by boat. The camp has two fishing boats which can be shared or privately hired.
Guided canoe trips (self-paddle or assisted) allow you to explore quiet corners of the island and soak up the tranquillity of Rubondo.
Experience the traditional dhows of East Africa. Go for an early morning sail to adjacent islands or to a remote beach picnic spot. Sundowner cruises are also available.
After a day of adventure, take some time to relax by the outdoor swimming pool overlooking Lake Victoria.

subset: commoncrawl


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Arrington Performance CF Intake *What size Pre-Filter fits this filter?
Anyone know off hand? I took some measurements, but can't find a "dry charger" or "pre charger" filter cover that would fit correctly.
There are a few K&N's that are close... here's my measurements:
7.50" Diameter
5.25" Length
4.75" Diameter
What brand & model number of filter is it?
Sent from my iPhone using Tapatalk
Re: Arrington Performance CF Intake *What size Pre-Filter fits this filter?
Not sure.... it's their "stock" filter that comes with the CAI.
Sent from my iPhone using Tapatalk
I just got off the phone with Arrington Performance and surprisingly they were unable to give me accurate dimensions for the filter. They did say it was made for them by Green Performance...
I'd like to know the answer to this if anyone has some ideas...
Anyone successfully add a filter wrap or per-charger?
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CANADA STOCKS-TSX slightly lower at open, Fed meeting in focus
TORONTO, April 28 (Reuters) - Canada's main stock index opened lower on Tuesday, with financial stocks leading the retreat, as investors shifted to the sidelines ahead of the U.S. Federal Reserve's policy meeting.
The Toronto Stock Exchange's S&P/TSX composite index fell 11.59 points, or 0.08 percent, to 15,332.49. Seven of the index's 10 main sectors slipped into the red, with financials off 0.1 percent. (Reporting by Solarina Ho; Editing by Jeffrey Benkoe)
© Thomson Reuters 2017 All rights reserved.


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I've always planned to monitor my electricity usage. I had previously (about a year ago!) ordered some power transformers from seeedstudio and a six channel ADC for simultaneous three phase current and voltage measurement but never got around to starting the project.
We've since had a "smart meter" installed which has a handy LED on it that flashes once every watt hour of energy is consumed. It also has and IrDA port on it but I believe the communications protocol is password protected and will report a tamper state if the password is tried too may times. Because of this, I decided it was best to optically interface with the meter, and best of all, I'll be measuring the exact amount of power we get billed for. Unfortunately I won't be able to predict the bill amount (in dollars) as the three phases that come into the house are billed at different rates while this LED pulses the combined usage.


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Andrew Frank
Telling stories that change the world.
Telling stories that change the world.
Welcome and thank you for your interest!
The purpose of this website is to engage my fellow citizens, students, clients and colleagues in discussions about important issues, and to keep you up-to-date on my latest projects in the classroom and the community.
Teaching in the Public Relations and Environmental Protection Technology departments at Kwantlen Polytechnic University (KPU), I combine active citizenship and research, with undergraduate teaching in public relations, communications, environmental issues and environmental legislation.
I invite you to connect with me here (using the form below), or on Twitter.
Sincerely and best regards,


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In 1996 the first of the Compact series of USP pistols entered production, and next year it was officially approved for German police use as the 9mm P10.
this information is from
Hey There,
I am curious if anyone can tell me when the first USP compact .40's became available. I was told 1996, then someone said it was early 1994. What I'm trying to figure out is wheather or not a preban 12 round magazine exists.
In 1996 the first of the Compact series of USP pistols entered production, and next year it was officially approved for German police use as the 9mm P10.
this information is from
Everyone knows you should never go full retail !
Thanks for the info, I guess my search for preban mags has ended.
There are HK 12 round mags that are unlabeled and not dated...the last ones I ordered came as such. You could go with those. Ones produced during the "ban" are stamped "law enforcement only"...I have some of those as well.
Why not just order some 12 rounders and see what they look like? Or would the Peoples Republik of Massachusetts have issue with that?
HK USPC .40 LEM w/MEP Tru-Dots
HK P2000SK .40 LEM w/MEP Tru-Dots
Mossberg 590A1 w/Knoxx SpecOps stock / red dot
Marlin 60 .22 rifle w/ Simmons Pro-Air 3-12X 40
Ithaca Model M49 .22 rimfire saddle rifle
Beeman R9 .177 break-barrel pellet rifle w/ Centerpoint
Marskman spring action pellet pistol
AR-15 project in progress...
Super-Soaker ;)
That works if the weapon was being manufactured before the 1994 AWB. In my state the politicians adopted that failure of a ban and all the same rules apply.
I looked on usacarry.com and it said there is no mag capacity restriction for a class A concealed permit holder...is that incorrect?
Concealed Permit:
May Issue to Residents and Non-Residents.
A Class A license authorizes carrying of handguns, including large capacity handguns, for all lawful purposes, subject to such restrictions relative to the possession, use or carrying of handguns as the licensing authority deems proper, and carrying of rifles and shotguns, including large capacity rifles and shotguns, for all lawful purposes, subject to restrictions imposed by the licensing authority. A class A license holder may carry a loaded handgun in a vehicle, if it is under his direct control. However, this does not apply to large capacity rifles or shotguns. Such firearms must be carried unloaded, in the locked trunk or in a locked case or other secure container.
Last edited by DiMora; 03-15-2010 at 01:38 AM.
The class A permit doesn't mean much in this state. possession of a postban magazine containing more than 10 rounds is a 10 year felony, the only exception is for law enforcement. This is usually easy to exploit as most of the manufactures don't add a date to the magazine. As I mentioned earlier, if the weapon was not in existence before September 1994, your out of luck.
Isn't it simply amazing what the State can use to shut your life down these days? I live in a "relatively" free place, but tales like that still piss me off to no end.
Mine aren't date-stamped, but the two 12rd mags that came with my USPc in .40 (purchased 2005) are all stamped "Law Enforcement Only" - never looked into Mass. carry laws to know if a date stamp is required to "prove" it isn't a post-ban mag.
I do know that it's up to the prosecutor to prove that the hi cap magazines are postban. The funny thing about this law is I've yet to meet a police officer that gave a **** about the law.


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About Through Hole Slip Ring
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With electrical energy rates soaring, as well as continuous lots dropping, these leading variation DIY wind turbines are the option to specifically when we are dealing with an energy situation worldwide. More and more people are currently utilizing this system as a different renewable resource as it generate an all-natural source of power that is environmentally friendly as well as is cheap to run as wind is cost-free.
These DIY wind turbines charge practically all kinds of batteries and also are exceptionally simple to set up. The wind generators with a top quality through hole slip ring can additionally give electrical power for your entire house devices, caravans, garages, camping area illumination without use of electrical energy. These wind generators do not launch carbon dioxide exhausts into the environment. There are numerous other dimensions that can be made use of in order to generate power and also are currently economical to the majority of people which will certainly supply you with a considerable level of electrical power.Watch Full Movie Online Streaming Online and Download
The kits are not specifically inexpensive, and also spend some time prior to they spend for themselves, a conserving will not be seen immediately however just a few months down the line. The advantage of the system is that it they supply power where no electric cables exist, as well as this alone is a fantastic conserving.
They are eco friendly and lower the carbon footprint. By mounting a wsystem, you will additionally be setting an instance to others, when they see you are eco-friendly as well as are environmentally friendly, like a style neighbours and also good friends more than likely will do the same.
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It is a great idea to take a look at the providers section on the web as this will certainly assist you to find a wind power system supplier in your area. It is also a good idea to do some research and also research the several advantages and disadvantages which relate to house and also business advancements so you will certainly obtain a standard understanding of just how it work. The distributors will certainly ship the DIY turbines system to all nations. Now is a great time for enthusiast to obtain in advance start by creating your own electricity. By switching over to making use of wind turbines with an excellent quality through hole slip ring we are making certain a safer environment for future generations.
Get the information about through hole slip ring you are seeking now by visiting http://www.barlintimes.com.


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Deerhunter Release Free Album
Listening to it now. Holy crap, is this different then their usual stuff. Intriguing, though, and
EDIT: Also, reviewing now.
Checked out the pink and blue albums (whatever they were called) but didn't really like them, I'll check this out though, since it's free
the pink album rules
EDIT: Also, reviewing now.
downloading now.
Ehh. I've been meaning to check these guys out. But I haven't. /busy busy busy
musicaddiction, this album is NOTHING like any of their work. I really don't know why this is even released under Deerhunter, as it's more fit for some random experimental side project.
weird stuff
i've never listened to these guys...always wanted to.
free=guaranteed listen
^^^ Haha, great, just don't get...turned off by this album.
For a second I thought this was Deerhoof and was like 'BITCHIN, BEST DRUMMER EVAR HERE I COME'
i love deerhoof =3 i can't wait until they do a tour around my area. i want to see them sooo bad
I remember when I saw Deerhoof open for Radiohead a few years back :D
These guys release stuff too fast for me to listen to!
• R.I.P. David Bowie• The Story So Far's vocalist dropk..• R.I.P. Lemmy• Brand new Brand New Single• The Fall of Troy bassist melts do..• Scott Weiland dead at 48 • Killswitch Engage debut new single• Deftones stream new single• Rush drummer retires• Riverside guitarist dies
• New Epica video/EP• New Visitors track + album• Dream Machine dropped by label• Novelists Release New Song • Melvins stream new track• TesseracT debut new single• New HAIM video• Captain, We're Sinking live at XP..• New LCD Soundsystem Album• New Fall Out Boy Song• 2nd Manchester Orchestra Song• Horns gesture trademark update• Turnover premiere "Super Natural"..• CityCop share music video• New Captain We're Sinking album s..» see all news
Bands: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z


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News By Tag
* Branding Agency
* Branding And Marketing
* Personal Branding Expert
* Personal Branding
* Branding Consultants
* Branding
* More Tags...
Industry News
News By Place
Industry News
Personal Branding Experts JW Dicks, Esq. and Nick Nanton Esq. Reveal The Biggest Business Mistake
JW Dicks, Esq., business strategist and personal branding expert, along with Nick Nanton, Esq., Agent to Celebrity Experts, recently wrote a piece for FastCompany about the biggest mistake that most business owners make.
In their latest blog post, titled "The Biggest Mistake Most Business Owners Make: Failure To Control The Conversation,"
"The majority of the time, more often than not, the only goal you should ever have for a first time visitor to your website is to capture their information by giving them access to something that will interest them enough to give you their most valuable commodity online besides their credit card, which is their contact information. Why? So you can control the conversation,"
"When someone visits your website they're interested in what you have to offer but they may not be ready to buy today. So it's our job to make them opt-in to your email list so you can control the communication, so that you can educate the prospect…so that when they're ready to buy they know that you're the most trusted resource," continued the authors.
To read the entire post and learn more, visit http://www.celebritybrandingagency.com/
JW Dicks is one of America's leading experts on using personal branding to build a business. JW emphasizes branding, and the importance of online marketing and social media to his clients and gives them strategies to expand their business in the current economy.
The strategy of developing and marketing business and professional experts into Celebrity Experts in their field to help them gain credibility and recognition for their accomplishments has led to Nick being known as "The Celebrity Agent." Nick's expertise on personal branding and marketing has been sought out and featured in the Los Angeles Times, Orlando Sentinel, The Chicago Tribune, The California Chronicle, DailyMe.com, FastCompany.com, USA Today, Newsweek and the Wall Street Journal, among others.
For more information on JW Dicks, visit http://www.CelebrityBrandingAgency.com
To learn more about Nick Nanton, visit http://www.NickNanton.com
About J.W. Dicks:
J.W. Dicks, Esq. is America's foremost authority on Personal Branding for Business Development. He has developed some of the most successful mass media and multi-channel business marketing campaigns in the country and built multi-million dollar businesses on the back of them – to the tune of more than $500,000,000 in sales.
J.W. represents some of the top marketers and professional experts in the world in the growth of their businesses using online and offline business development systems, social media, multi-dimensional marketing, franchising and strategic legal structure to accomplish their goals and capitalize on the assets they create.
A "Best Selling" author with more than 14 published books, and hundreds of articles, J.W. has also been quoted or appeared in Newsweek, The Wall street Journal, USA Today, NBC, ABC, CBS, and FOX affiliates as well as Entrepreneur'
In addition to coaching and consulting for clients nationwide, J.W. is also a successful entrepreneur living in the trenches himself. He has built his own businesses, with annual sales exceeding $35 Million, developed real estate in excess of $200 Million and created and sold intellectual property rights for as much as $1.8 Million.
J.W. is a graduate of the University of Florida and George Mason College of Law. He is a member of the American Bar Association, NASD, National Association of Realtors, the Florida Bar and the Virginia Bar.
J.W.'s business address is in Orlando, and his play address is at his beach house where he spends as much time as he can with his wife of 37 years, Linda, and their two Yorkies. His major hobby is fishing… although the fish are rumored to be safe.
firstname.lastname@example.com • 800.980.1626
About Nick Nanton, Esq.:
Nick Nanton, Esq. is known as The Celebrity Lawyer and Agent to the top Celebrity Experts for his role in developing and marketing business and professional experts, through personal branding, to help them gain credibility and recognition for their accomplishments. Nick is recognized as the nation's leading expert on personal branding as Fast Company Magazine's Expert Blogger on the subject and lectures regularly on the topic at the University of Central Florida. His book Celebrity Branding You® has been selected as the textbook on personal branding at the University.
Nick is an award winning songwriter and television producer and has worked on everything from large scale events to television shows with the likes of Bill Cosby, President George H.W. Bush, Brian Tracy, Michael Gerber and many more.
Nick is recognized as one of the top thought leaders in the business world and has co-authored 10 best-selling books, including the breakthrough hit Celebrity Branding You!®.
Nick serves as editor and publisher of Celebrity Press™, a publishing company that produces and releases books by top Business Experts. CelebrityPress has published books by Brian Tracy, Mari Smith, Ron Legrand and many other celebrity experts and Nick has led the marketing and PR campaigns that have driven more than 100 authors to Best-Seller status. Nick has been seen in USA Today, The Wall St. Journal, Newsweek, Inc. Magazine, The New York Times, Entrepreneur®
Nick is a member of the Florida Bar, holds a JD from the University of Florida Levin College of Law, as well as a BSBA in Finance from the University of Florida's Warrington College of Business. Nick is a voting member of The National Academy of Recording Arts & Sciences (NARAS, Home to The GRAMMYs), co-founder of the National Academy of Best-Selling Authors, a 4-time Telly Award winner, and spends his spare time working with Young Life, Florida Hospital and rooting for the Florida Gators with his wife Kristina, and their two sons, Brock and Bowen.
# # #
The Dicks and Nanton Branding Agency helps businesses brand themselves online by elevating their expert status through the use of television, radio and feature publications.
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Stairway Help Needed
I need to build a steep stairway inside a garage. I am limited to 146" for the total run. The total rise is 132". Can someone help with the rise and tread? All help is very appreciated.
16 stairs @ 8.25" tall X 9" deep.. Hope that helps
i hope you didn't build your stairs yet.
There is always one less run than rise. The correct answer is 16 rises at 8.25" and 15 treads at 9.73". However, you may want to check with your building codes, I think minimum on a tread is 10" and max on a rize is 8".
Good luck ~ Gooshimo online ~
I do believe you're right. As far as I know it's been 8" on the rise and 10" on the tread. I don't think so, but perhaps your state is different. If that doesn't fit perhaps you can make your opening a little larger. How tall is the ceiling. Maybe we can suggest another idea.
Two steps to a platform with a 90 degree turn put me into compliance one time when space was a restriction.
RSRS=17 R-SZ=7.75"overage underage .25" 16 treads,T-SZ=9.25" overage underage 2" stringer length 191.75" degree of inclination=40.4
RSRS=16 R-SZ=8.25" overage underage=0" TRDS=15 T-SZ=9.75" overage underage=.25" stringer=191.5" degree of inclination=40.3:)
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April 24, 2014
BRIDGEPORT, Pa. -- University of Michigan freshman Allison Skaggs (Lake Forest, Calif./El Toro HS) was named the Collegiate Water Polo Association (CWPA) Western Division Rookie of the Year, the league announced Thursday (April 24) on the eve of the annual CWPA Championship. She was also one of three Wolverines named to the all-division teams; senior Kelsey Nolan (San Jose, Calif./Leland HS) was named to the first team, while Skaggs and sophomore Presley Pender (Costa Mesa, Calif./Newport Harbor HS) earned second-team honors.
Skaggs has appeared in all 31 games this season for the Maize and Blue and owns 31 goals, 11 assists and 42 points in her rookie campaign. She ranks third among U-M's goal-scoring leaders -- first among the Wolverines' seven freshmen field players. She was named the CWPA Rookie of the Week three times during the regular season.
Skaggs is the third player in Michigan program history to earn the league's top rookie honor and the second in as many years, joining sophomore Bryce Beckwith (Sacramento, Calif./St. Francis HS), who garnered the 2013 honor, and Shana Welch, who claimed the award in 2004.
Nolan earned CWPA All-Western Division first-team accolades for the second time in her career, adding to her 2012 honor, and garnering second-team distinction last season. Nolan ranks second on the U-M team with a career-best 42 goals and 18 assists. Nolan, who has also twice earned CWPA Easterns All-Tournament honors, owns 129 goals, 92 assists and 221 points in her career, nearing U-M's top-10 all-time leaders in all three categories.
Pender currently leads the Wolverines with 45 goals, 36 assists and 81 assists -- career highs in all three categories -- and on track to become just the fourth player in program history to boast the most goals and assists in a single season. Pender has scored in 16 of the last 18 games, including multiple goals in 12.
Voted on by the head coaches in the division, the all-division team comprises the best players based on nominations from the coaches, combined with a review of each player's performance during the 2014 regular-season and divisional play.
The Wolverines will kick off the postseason this weekend, traveling to Lewisburg, Pa., for the annual CWPA Championship. As the No. 4 seed, the Wolverines will open play against No. 5-seeded Brown (18-20) at noon on Friday (April 25) at Bucknell's Kinney Natatorium. Depending on the outcome, U-M will play at either 1:30 p.m. or 4:30 p.m. against either Indiana or Connecticut College on Saturday (April 26). The championship game is scheduled for 1:30 p.m. on Sunday (April 27).
Player of the Year: Shae Fournier, Indiana
Coach of the Year: Barry King, Indiana
Rookie of the Year: Allison Skaggs, U-M
All-Western Division First Team
Jessica Gaudreault (Goalie), Indiana
Shae Fournier, Indiana
Allison Campbell, Indiana
Rebecca Gerrity, Indiana
Jemma Dendy Young, Hartwick
Sami Capparelli, Hartwick
Kelsey Nolan, U-M
All-Division Second Team
Michaela Davies (Goalie), Hartwick
Katelyn Jacobs, Gannon
Shelby Taylor, Indiana
Candyce Schroeder, Indiana
Presley Pender, U-M
Allison Skaggs, U-M
Gina-Bella Mata'afa, Notre Dame College
Contact: Leah Howard


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The Divisional Round is now complete, and there was finally a road win. And were down to just the final four. Next Sunday, it'll be Patriots/Ravens and 49ers/Giants for the right to play for the Lombardi Trophy. After the previous round, we're sure to see plenty of action.
The Saints threw plenty of people for a loop, but other than that, it was easy enough to pick the games. Some of the yardage and touchdown totals were a bit surprising, but generally easy enough.
The standings are starting to open up a little, but there's still three rounds to go. So check below for the results from the previous round, and check back Sunday for the Conference Championship picks. Continue reading 2012 NFL Playoff Expert Picks: Divisional Round Results »
Tags: NFL Pick 'Em, NFL Picks, NFL Playoffs


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Estate agents in Sittingbourne
Featured agents
Bairstow Eves Countrywide
93 High Street, Sittingbourne, ME10 4AJ - We also cover ME9
68 High Street Sittingbourne, Kent, ME10 4PB
Award-winning Connells was founded in 1936 and is one of the country's leading estate agents ... more
Family Homes
25a West Street, Sittingbourne, ME10 1AL - We also cover ME12, ME13, ME8 and ME9
Family Homes are Independent Sales, Lettings and Property Management Specialists. Our offices ... more
Geering & Colyer
43 Court Street, Faversham, ME13 7AL - We also cover ME9
About us Established over 100 years ago, we pride ourselves on being a traditional estate ... more
Harrisons Residential
2 West Street, Sittingbourne, ME10 1AW
Here at Harrisons we've always loved a new beginning. It's something we've been proud to deliver ... more
J's Sales & Lettings
5 Alefe Way, Iwade, Sittingbourne, ME9 8TX - We also cover ME10 and ME12
We are a local company owned and run by local people Jane, Debbie and Jill are waiting for your ... more
Lamborn & Hill Estate Agents
19 - 21 West Street, Sittingbourne, ME10 1AJ
Lamborn & Hill estate agents opened the Sittingbourne office in 2009. The business quickly ... more
Mann - Rainham
11 High Street, Rainham, ME8 7HX - We also cover ME9
Specialising in property sales and lettings across Southern England, Mann has an extensive ... more
Mann - Sittingbourne
3 Central Avenue, Sittingbourne, ME10 4BX - We also cover ME9
Specialising in property sales and lettings across Southern England, Mann has an extensive ... more
Quealy & Co
Times Chambers, Sittingbourne, ME10 1DR
Established in 1989, Quealy & Co are specialists in all aspects of buying, selling, letting, ... more
Robinson Michael & Jackson - Sittingbourne
38 West Street, Sittingbourne, ME10 1AP
Request valuations and viewings 24/7. Personal Service & NO UPFRONT ONLINE FEES. If you're ... more
Your Move
28 West Street, Sittingbourne, ME10 1AP
Buckey and Ward
23 West Street, Sittingbourne, ME10 1AJ
Buckey & Ward are the longest established Independent Estate Agent in Sittingbourne, dealing ... more
Fresh Estates
151 London Road, Teynham, ME9 9QJ
George Webb Finn
43 Park Road, Sittingbourne, ME10 1DX
If you are considering renting your property or are looking for a new home we at George Webb ... more
Hawkesford James
26 West Street, Sittingbourne, ME10 1AP
Hawkesford James is an Independent Estate and Letting Agent specialising in residential Sales & ... more
The Letting Centre Ltd
16 West Street, Sittingbourne, ME10 1AW
Ward & Partners
13/15 West Street, Sittingbourne, ME10 1AJ
Our marketing approach is unrivalled and will reinvigorate interest in your home. Make your ... more


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The Smoothies Bible Second Edition
Use smoothies to get your daily fruit and vegetable requirement.Top Rated Diets of 2017
The Smothies Bible second edition is a book written by Pat Crocker that includes over 400 healthy smoothie recipes. Pat Crocker is a culinary herbalist and professional home economist. She has written and demonstrated about herbs and health issues for over 25 years. There are also over 100 illustrated profiles of fruits, vegetables and herbs. The 80 most common health concerns are dealt with along with what healing smoothies you can use to help treat that health concern. This book showcases how smoothies are rich in antioxidant fruits and vegetables. They are also a fast, easy and great-tasting way to achieve good health. Following the recipes in this book can give you a smoothie remedy for just about anything. You will be using whole, organic fruits and vegetables to make your smoothies.
There are different types of smoothies including those that are served hot, cold, at room temperature or frozen. All smoothies need a liquid base which might include juice, milk, stocks, broth or herbal tea. Smoothies can contribute to a healthy diet by making it easier to consume the recommended five to eight daily servings of fruits and vegetables. One large glass of pure, raw or lightly cooked, blended fruits or vegetables will help improve your immune system, increase energy, strengthen bones, clear skin and lower the risk of disease.
Do You Know the Best Diets of 2017?
- Several health benefits of smoothies
- Makes it easier to get the recommended five to eight servings of fruits and vegetables daily
- Some smoothies can directly help with common diseases
- Recommends smoothies as part of your daily routine
- Outlines the equipment you need for smoothies
- Offers information on healthy body systems
- Gives information on all fruits and vegetables included in the various recipes
- Nutrition info is not included on recipes
- Very few smoothies combine fruits and vegetables
The Smothies Bible is a book that shows you how to use smoothies to improve your health, help you manage certain diseases and get the recommended amount of fruits and vegetables into your diet. There are some guidelines in the book to eating well which include eat a minimum of five servings of fruit and vegetables every day, focus on the most colorful fruit and vegetables, choose whole grains over processed grains, limit refined carbohydrates and cook with olive or organic canola oil. Having a smoothie is a great way to have all of your fruits and vegetables in one quick drink. There are various common diseases addressed in The Smothies Bible and you are given several smoothies that will help you manage and in some cases improve them. Some of the diseases highlighted include:
- Arthritis
- Breastfeeding
- Common Cold
- Diabetes
- Fibromyalgia
- Heart Problems
The Smothies Bible includes over 400 delicious recipes, so you won't get bored and you should have no problem finding several recipes to meet your needs. The recipes are divided into sections and listed alphabetically. Your choices include fruit smoothies, vegetable smoothies, healing herb smoothies, dairy smoothies, dairy alternative smoothies, hot and frozen smoothies, and dessert and cocktail smoothies. Some of the smoothie recipes in The Smothies Bible include:
- Apple Fresh
- Leafy Pear
- Hot Watermelon Cooler
- C-Green
- Nectarlicious
- Squash Special
- Mochaccino
- Allium Antioxidant
- Good Health Elixir
The Smothies Bible is a book about smoothies so there is no outlined exercise plan within the book. There is a tidbit of information for those that are overweight and looking to smoothies to help aid in weight loss. The book recommends that you exercise daily according to your fitness level. There is also a section at the beginning of the book that calls out guidelines to good health. It suggests that moderate daily activity can lower cancer risk, boost the immune system, help prevent obesity, decrease estrogen and insulin growth factor and improve overall health and emotional well-being.CONCLUSION
The Smothies Bible can be a great tool for those interested in incorporating more fruits and vegetables into their diet. This book has a lot of smoothie recipes and can be beneficial to those with diseases and can help you manage and treat them naturally.Common Misspellings
smooth bible, smoothie bible, smoothy bible, smoothey bibleHow Does The Smoothies Bible Second Edition Compare?
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Don Coleman (linebacker)
|Linebacker, fullback, defensive end
|Personal information
|Date of birth:
|January 11, 1952
|Place of birth:
|Toledo, Ohio
|6 ft 2 in (1.88 m)
|222 lb (101 kg)
|Career information
|High school:
|Toledo St. John
|NFL Draft:
|1974 / Round: 16 / Pick: 398
|Career history
|Player stats at PFR
Donald Alvin Coleman (born January 11, 1952) is an American entrepreneur, advertising executive and a pioneer in the growing field of multicultural advertising. Coleman is the founder, chairman and CEO of GlobalHue, the largest multicultural advertising agency in the United States, working with blue chip brands and organizations to communicate with African-American, Asian and Hispanic consumers. He is also a former American football player, having played college football at the University of Michigan from 1971 to 1973 and professional football for the New Orleans Saints and New York Jets from 1974 to 1978.
Early years[edit]
Coleman was born in Toledo, Ohio in 1952,[1] and grew up in that city's Old West End neighborhood. His mother, Dorothy Coleman, worked for the Department of Housing and Urban Development, and his father, Augustus Coleman, worked as a skycap for United Airlines and as a janitor at a library.[2]
At the suggestion of teachers at his public school, who told his parents that Coleman was not sufficiently challenged, he transferred to St. John's Jesuit High School and Academy. He attended St. John's from 1966 to 1970 where he excelled in academics and sports. Coleman was an All-City and All-State running back for St. John's and was recruited by multiple colleges to play football. He later recalled: "When it came time to select a university, I had about 20 coaches visiting the house, talking up their programs. I chose the University of Michigan. I received a full scholarship."[2]
University of Michigan[edit]
Coleman enrolled at the University of Michigan in 1970 on a football scholarship. He played college football for the Michigan Wolverines under head coach Bo Schembechler from 1971 to 1973. Coleman began the 1971 season as a backup fullback, but Schembechler moved him to defensive end in late September 1971.[3][4] Coleman played at defensive end from 1972 to 1973 and helped the Wolverines tie for first place in the Big Ten Conference both years.[5][6][7] Although still listed as a defensive end, Schembechler described Coleman's role as follows in 1973: "In our defense we use a flop-end. So Coleman is actually a pro linebacker. He has to cover the pass as part of his responsibility today more so than normal."[8] Coleman had 51 tackles and 52 assists for the Wolverines.[9]
Professional football[edit]
Coleman was drafted by the New Orleans Saints in the 16th round (398th overall pick) of the 1974 NFL Draft. He played two seasons for the Saints in 1974 and 1975, appearing in 27 NFL games.[1] Coleman was cut by the Saints in August 1976.[10] He signed as a free agent with the New York Jets shortly after being released by the Saints,[11] but he missed the entire 1976 NFL season with a left knee injury. In August 1977, Coleman underwent surgery on his right knee,[12] and he was placed on injured reserve status.[13] The Jets released Coleman in June 1978.[14]
Advertising career[edit]
While recovering from football injuries, Coleman obtained an M.B.A. degree in marketing from Hofstra University in New York in 1976.[15] In 1978, he was hired as an assistant account executive at the Campbell-Ewald advertising agency in Detroit. He later recalled: "I was the only black person in account management in the office. It was boring work, but I was speedy and accurate, persevered and became a full account executive."[2] In 1983, he became senior vice president and management supervisor on the Chevrolet account.[2]
Coleman next began a career in advertising directed to minority communities. He was hired in 1983 as a senior vice president and account director for Burrell Advertising, a Chicago firm specializing in advertising to minorities.[2]
In 1988, he formed Don Coleman Associates, which became Don Coleman Advertising in 1991. He sold a minority interest in the company and bought two additional agencies, one focused on the Asian-American market (Innovasia Communications) and one focused on the Hispanic market (Montemayor y Asociados). He combined the companies into GlobalHue, specializing in a culture based approach to marketing. He is the chairman and chief executive of GlobalHue.[2]
In 2009, GlobalHue's capitalized billings were $833.7 million. The company has approximately 300 employees in Southfield, Michigan and New York City. Its clients include Subway, the United States Navy, U.S. Census 2010, Verizon Wireless, Merck & Co., Inc., Walmart and Chrysler Group.[15] In 2009, GlobalHue was named "Multicultural Advertising Agency of the Decade" by Advertising Week magazine.[16][17][18] In 2010, Black Enterprise magazine named GlobalHue the BE 100 Advertising Agency of the Year, an honor that GlobalHue also received in 1998 and 2003.[19] Coleman's daughter, Kelli Coleman, has also worked for GlobalHue since 2002 and is the vice president for business development.[20]
In 2009, Coleman launched The Coleman Entrepreneurial Scholarship (CES), which strives to promote entrepreneurship as a career choice among college students of ethnic backgrounds, particularly African-American and Hispanic.[21] CES awards $50,000 in scholarships annually.[22]
Coleman is also a member of the External Advisory Board at the A.C. Nielsen Center Marketing Research Business School, the Board of Visitors at The Howard University John H. Johnson School of Communications, and the Board of Trustees for the Charles H. Wright Museum of African American History[23] and the Louis Carr Internship Foundation. Since 1999, he has served as vice chairman of the National Action Network (NAN), where he is a contributor to the organization's fundraising initiatives.
- 2010 Best of Black Business Advertising Award
- 2010 Ranked #31 in the list of the "Top 40 Titans: The Most Powerful African Americans in Business" by Black Enterprise magazine
- 2009 Moss Kendrix Marketer Award from the National Alliance of Market Developers
- ^ a b "Don Coleman". pro-football-reference.com.
- ^ a b c d e f Don Coleman (April 17, 2010). "Boss: Lessons From the Gridiron". The New York Times.
- ^ "Michigan Shifts Coleman To End". The Blade, Toledo, Ohio. September 29, 1971.
- ^ "U-M's Grambau Making Comeback". The Owosso Argus-Press. September 29, 1972.
- ^ "1972 Football Team". University of Michigan, Bentley Historical Library.
- ^ "1973 Football Team". University of Michigan, Bentley Historical Library.
- ^ "University of Michigan All-time Rosters Search Page". University of Michigan, Bentley Historical Library. Archived from the original on 2007-09-28.(Enter "Coleman" for last name and "Donald" for first name on the search page, and press enter)
- ^ "'Cute' defenders make the big play". The Michigan Daily. September 25, 1973.
- ^ "Michigan Football Statistic Archive Query Page". University of Michigan. Archived from the original on November 12, 2007. Retrieved July 20, 2011.(To access Coleman's record, enter "Don" as his first and "Coleman" as his last name in the main search page. Then click on "Display Stats By Season" to access his game-by-game and season-by-season results.)
- ^ "The Scoreboard". Daily Sentinel. August 5, 1976.
- ^ "Coleman Is Signed". The New York Times. August 21, 1976.
- ^ "Knee surgery". The Spokesman-Review. August 27, 1977.
- ^ "Rookie Leads Giants To Win". The Evening News. August 30, 1977.
- ^ "Sports Transactions". The Sumter Daily Item. June 17, 1978.
- ^ a b "Donald Coleman". The History Makers.
- ^ "MULTICULTURAL AGENCY OF THE DECADE: GlobalHue". Advertising Week.
- ^ Mike Chapman (January 14, 2010). "Q&A: GlobalHue's Don Coleman". Advertising Week.
- ^ "Making Cultural Connections". Black Enterprise.
- ^ "WATCH: 2010 BE 100s Award Winner". Black Enterprise. May 19, 2010.
- ^ "Twenty in the Twenties: Kelli Coleman, 23". Crain's Detroit.
- ^ http://www.blackenterprise.com/b-e-100s-rename-test/be100s-news/2009/02/03/advertising-mogul-creates-scholarship-to-promote-entrepreneurialism/
- ^ http://www.targetmarketnews.com/storyid02040903.htm
- ^ "Archived copy". Archived from the original on 2010-07-13. Retrieved 2010-10-12.


text:
|News Events Culture Video Photo Gadget Shopping Recipes Tour Street Weather
Where we are
The association "OLL MUVI is located in Molfetta, without exaggeration, the most beautiful town in the world for those who were born and for those who, for many different reasons have had the great fortune to live for a short period of time. Surely, Molfetta, like all the other towns' in the world, has its negative sides, but thanks to the liveliness' of its people, the ability 'to fall and rise again and the stubbornness' of its inhabitants it makes Molfetta a unique town. We say that where ever you go you'll always find a piece of Molfetta somewhere.
For this I LOVE MOLFETTA invites all its web visitors to come and meet us. It just takes a few days to fall in love with the town. This town is like a woman or a man that enters your blood, perhaps it makes you suffer, it tears your soul, but you just can't live without it.
Molfetta is an important fishing center, and it is only a handful of kilometers away from the north of Bari. It has an old town centre kept well and recently restored. It is continuosly expanding infact in the last decades there have been numerous zones of expansion to the point that the town has grown four times more in half a century. It has an ancient history infact human traces of settlement going back to the prehistoric age, were found. According to some sources "Molfetta a was flourishing center under the Svevis, the Angioini and the Aragoneses. It was a boarding harbour towards the holy Land, and shelter for the Venetian ships of the Serenissima one and as well those of Ragusa, in Dalmatia. Even amalfitani merchants, Slavic and Greek built lodges and warehouses. During Charles of Bourbon's reign the town went through a flourishing period of political and economic stability. Umberto I set up an industrial development of big proportions. Also today Molfetta is at the center of a wide industrial area with important shopping centers. The town is a fertile background where talented people are helped emerge in every field.
© Associazione culturale "Oll Muvi" - Molfetta - CF 93369320721


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'Cooking Light's' L.E.O. Scramble Recipe
Recipes from Cooking Light Lighten Up, America! by Allison Fishman Task
Click here to purchase Cooking Light Lighten Up, America!
L.E.O. Scramble
L.E.O. refers to the winning combination of lox, eggs, and onions in
this savory scramble. It's a New York diner favorite that's easy to prepare at
home. In my lighter version, I simply
substitute egg whites for some of the whole eggs and reduced-fat cream cheese
for the full-fat variety.
- Yield: Serves 4 (serving size: 3/4
cup scramble and 1 toast slice)
- Total:11 Minutes
- 6
large eggs
- 4
large egg whites
- 1
teaspoon canola oil
- 1/3
cup sliced green onions
- 4
ounces smoked salmon, cut into 1/2-inch pieces
- 2
ounces reduced-fat cream cheese (about 1/4 cup), cut into 12 pieces
- 1/4
teaspoon freshly ground black pepper
- 4
(1.1-ounce) slices pumpernickel bread, toasted
1. Place eggs and egg whites in a bowl; stir with a whisk until blended.
2. Heat a medium nonstick skillet over medium-high heat. Add oil to pan;
swirl to coat. Add green onions to pan; sauté 2 minutes or until tender. Add
egg mixture to pan. Cook without stirring until mixture sets on bottom. Draw a
spatula across bottom of pan to form curds. Add salmon and cream cheese.
Continue drawing spatula across bottom of pan until egg mixture is slightly
thick but still moist; do not stir constantly. Remove from pan immediately.
Sprinkle egg mixture with pepper. Serve with pumpernickel toast.


text:
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C-9A Polished Unlacquered Brass Antique Reproduction Heavy Victorian Style Cast Paw Foot Casters
C-DK9A Antique Brass Finish Heavy Victorian Style Cast Paw Foot Casters
Finer casters cannot be found. Carefully sand cast with precision cores and finished through 5 processes. Internal measurements can vary slightly due to the nature of sand casting.
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Inside Opening Dimensions:
Brass Wheel Diameter: ¾"
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Vintage Hardware & Lighting ~ 2000 W Sims Way ~ Port Townsend, WA 98368 ~ 360-379-9030
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The Scarlet Son
Recently traveling to Long Island NY to record the track "Consumed" with producer Mike Watts at Vudu Studios, The Scarlet Son takes no shortcuts for a new take on music. From Carnegie PA, The Scarlet Son was formed by Guitarist/Vocalist Kris Litman alongside fellow guitarist Dave O'Loughlin. Together, a vision for quality music was formed that they want to share with as many ears as possible.


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Junior Member
Last Activity: 04-28-2013 01:53 PM
About Me
- About pff2008
- Biography
- I am a stay at home mom of 4 children. I have 3 beautiful yorkies too.
- Location
- southern illinois
- Interests
- I love walking my yorkies! great for me and them both. I love camping, reading, fishing,
- Occupation
- I work for microsoft from the comfort of my home.


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General Question
I found a lump on my 4 month old chihuahua?
Asked by Tequila (334) February 22nd, 2013
My chihuahua is 17 weeks old and I just found a dime-sized lump in her right hip area. She is sleeping, eating, playing normally but I am worried about what this may be and if she's going to be okay. I know taking her to the vet is the best thing to do but I am broke and I'd like to avoid that if at all possible. She just got the last of her "puppy shots" last week. Does anyone have any experience with this on such a young dog? Any ideas?
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Baseballs to the Wall
If you've ever attended a Major League baseball game, you might have noticed most of the music is terrible. Most players enter the game to bad rap, jock metal, butt rock, or country. In an average game, a crowd will be exposed to more than 75 snippets of modern music. The shoddy music is tolerable because it blends into the fabric of the game. Given enough time, Five Finger Death Punch fades into Lil' Jon … which fades into Toby Keith … and you get Five Finger Lil' Toby Keith.
However, there is one time during a baseball game when a player's entrance/introductory music matters. When a closer takes the mound, it's one of the most exciting moments in a Major League baseball game. As the closer leaves the bullpen and jogs toward the mound, if he's at his home stadium, the PA begins to blare his entrance song. The music helps to pump up the crowd and the pitcher – or at least that's the theory. Over time, the closer's song becomes indelibly linked to the closer himself.
Given metal's characteristics, it shouldn't be surprising that a number of closers have used songs from metal or related genres. Mariano Rivera, for instance, famously jogged out to "Enter Sandman" for years. The song fit him perfectly: his signature cut fastball put fear into batters' hearts, and then it put them back on the bench. My current favorite closer/music combo is John Axford of the Brewers. Axford enters the game to the Refused's "New Noise," which means he Wins The Internet.
Here are a few other metal/hardcore/punk songs used by closers:
AC/DC – "Hell's Bells"
Killswitch Engage – "The End of Heartache"
Guns N' Roses – "Welcome to the Jungle"
Deep Purple – "Smoke on the Water"
Nine Inch Nails – "Hurt" (also not metal, but seriously, NIN! In a baseball game!)
Rage Against the Machine – "Killing in the Name"
Dropkick Murphys – "Shipping Up to Boston" (appropriately, it's used by the Boston Red Sox's former closer)
Alright, here's the deal: In 2012, at least a dozen teams have lost or replaced a closer due to injury or ineffectiveness. Numerous new closers will jog to the mound this year, and they each need songs. If you could choose a closer's entrance music, what song would he use?
Oh, and don't forget, your song will be played on sunny summer Sundays in front of thousands of ballpark attendees and hundreds of thousands of television viewers. I once went to a baseball game with a coworker and his son. The son asked who Thor was, and my coworker answered, "A comic book character". He didn't want his son to have any inkling that another culture worshipped Thor as a god.
Keep that guy in mind when you choose your song. If you offend him, he doesn't show up, and if he doesn't show up, your team doesn't get paid. That means no cussing, no gore, and no sex. Dirty/harsh vocals are fine, but only up to a point. Demilich and Ashdautas are out of the question.
Here are my top five choices:
. . .
Kataklysm – "The Ambassador of Pain" from Serenity in Fire
. . .
The music's a mix of blasting to get our closer and the crowd pumped, and groove to help our closer establish his rhythm. It's perfect for a loose cannon. The song says, "I throw 100 mph and I don't know where it's going, but I can still drill you in the fucking ribs anytime I want to."
. . .
Hollenthon – "Woe to the Defeated" from With Vilest of Worms to Dwell
. . .
I'd only use the first 1:45 or so of "Woe", which features male choirs chanting. It's big, bold, bombastic, and energizing. It's also perfect for warming up: it starts with a stately, methodical bounce, and then the guitars drop in and things get fast and exciting. Also, how awesome is it to tell your opponent that you feel woe for them before you defeat them?
. . .
Iron Maiden – "Sun and Steel" from Piece of Mind
. . .
First, this song is stupidly catchy. Second, I think there's a parallel between the song's subject, Yukio Mishima, and a MLB pitcher. Both are freaks of nature trained to near-perfection, and both live on the razor's edge between success and total failure. Both make their legends via a series of duels. I can see an elite closer running to the mound to "Sun and Steel," the sun on his back, a twinge in his elbow, knowing that today could be his last.
. . .
Pharaoh – "I am the Hammer" from The Longest Night
. . .
Yup, some power metal. What'd you expect from me? Anyway, the curve ball has many nicknames, the best of which is The Hammer. Closers are hammers, nailing down the end of the game. Calling yourself The Hammer sounds awesome. Need I go on? Here are the opening lyrics: "Forged in fire, but cold as ice / I will spare no sacrifice / You'll pay with tears shed in mortal fright / All shall fear the hammer's strike."
. . .
Penderecki – "Polymorphy"
. . .
This piece is fucking terrifying. Terrifying is one of Penderecki's calling cards, along with discomfiting, noisy, ominous, and evil. Use the parts from 7:00 to the end, and the stadium will feel like Death just ran His finger down every spine in attendance. Make sure your closer's not intimidated, too. The major chord swell at the end just fucks with people's heads.
Now it's your turn. What songs would your closer use? Will he have an intimidating piece with swagger? Music that is raw rage? "O Fortuna"? Let us know in the comments!
. . .
At this point, some of our foreign readers are probably flummoxed. I can't explain baseball to you. Here's a throwaway alternative: When your football or ice hockey team goes to a sudden death shootout or penalty kick/shot, they play a song for the shooter. Choose the song.
. . .


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1Department of Structural Biology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine
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We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.
Date Published: 6/24/2013, Issue 76; doi: 10.3791/50386
Keywords: Bioengineering, Issue 76, Molecular Biology, Structural Biology, Virology, Biophysics, Cellular Biology, Physiology, Medicine, Biomedical Engineering, Infection, Microbiology, Technology, Industry, Agriculture, Life Sciences (General), Correlative microscopy, CryoET, Cryo-electron tomography, Confocal live-cell imaging, Cryo-fluorescence light microscopy, HIV-1, capsid, HeLa cell, cell, virus, microscopy, imaging
Jun, S., Zhao, G., Ning, J., Gibson, G. A., Watkins, S. C., Zhang, P. Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions. J. Vis. Exp. (76), e50386, doi:10.3791/50386 (2013).
Cryo-electron tomography (cryoET) allows 3D visualization of cellular structures at molecular resolution in a close-to-physiological state1. However, direct visualization of individual viral complexes in their host cellular environment with cryoET is challenging2, due to the infrequent and dynamic nature of viral entry, particularly in the case of HIV-1. While time-lapse live-cell imaging has yielded a great deal of information about many aspects of the life cycle of HIV-13-7, the resolution afforded by live-cell microscopy is limited (~ 200 nm). Our work was aimed at developing a correlation method that permits direct visualization of early events of HIV-1 infection by combining live-cell fluorescent light microscopy, cryo-fluorescent microscopy, and cryoET. In this manner, live-cell and cryo-fluorescent signals can be used to accurately guide the sampling in cryoET. Furthermore, structural information obtained from cryoET can be complemented with the dynamic functional data gained through live-cell imaging of fluorescent labeled target.
In this video article, we provide detailed methods and protocols for structural investigation of HIV-1 and host-cell interactions using 3D correlative high-speed live-cell imaging and high-resolution cryoET structural analysis. HeLa cells infected with HIV-1 particles were characterized first by confocal live-cell microscopy, and the region containing the same viral particle was then analyzed by cryo-electron tomography for 3D structural details. The correlation between two sets of imaging data, optical imaging and electron imaging, was achieved using a home-built cryo-fluorescence light microscopy stage. The approach detailed here will be valuable, not only for study of virus-host cell interactions, but also for broader applications in cell biology, such as cell signaling, membrane receptor trafficking, and many other dynamic cellular processes.
Cryo-electron tomography (cryoET) is a powerful imaging technique that allows three-dimensional (3D) visualization of cells and tissues and provides insights into the organization of native organelles and cellular structures at molecular resolution in a close-to physiological state1. However, the inherently low contrast of unstained frozen-hydrated specimen, combined with their radiation sensitivity, makes it difficult to locate areas of interest inside a cell and subsequently performing the tilt series successfully without damaging the target area. In order to overcome these problems, a correlative approach that combines light and electron microscopy is necessary. Specific features highlighted by fluorescent labeling are identified and located by fluorescence light microscopy, and then their coordinates are transferred to the electron microscope for acquisition of high resolution 3D structural data. This correlative method helps locating the target areas of interest to be addressed. Due to the limitation on sample thickness with cryoEM (<300 nm), currently only the peripheral regions of the cell are suitable for 3D structural analysis by CryoET. Further reducing the thickness of frozen-hydrated specimens by vitreous sectioning8 or by cryo-focused ion beam (FIB) milling9 would expand the capability of correlative imaging.
Previously, correlative methods were primarily used to facilitate cryoET data acquisition for large and static structures10-13. In these studies, cryo-stages have been implemented to accept cryoEM grids and fit onto either an upright microscope or an inverted microscope10,11,14. Although switching grids seems fairly straightforward in their designs, there are additional transferring steps involved for the EM grid, increasing the chance that the grid may be deformed, damaged and contaminated. We recently demonstrated a technical advance in correlative microscopy that allows us to directly visualize dynamic events that are by nature difficult to capture, such as HIV-1 and host cell interactions at early stages of infection15. We accomplished this by designing and implementing a cryo-light microscopy sample stage that adapts a cartridge system to minimize the specimen damage due to grid handling, thus facilitating correlation. Our design includes an integrated specimen cartridge holder, allowing both cryo-light and cryo-electron microscopy to be performed, sequentially, on the same specimen holder, without sample transfer, thus streamlining the correlative process. In addition, we also implemented an accurate and reliable correlation procedure using fluorescent latex beads as fiducial markers.
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1. HeLa Cell Culture on Carbon-coated, Gold EM Finder Grids
2. HIV-1 Infection and Live-cell Imaging
3. Frozen-hydrated EM Sample Preparation
To minimize the time delay between collection of the last confocal image and cryo-fixation of the sample, the FEI Vitrobot (or other vitrification device) should be initiated and prepared for plunge-freezing during collection of fluorescence confocal live-cell images.
4. Cryo-fluorescence Light Microscopy
A home-built, cryo-fluorescence sample chamber and stage system (Figure 3) is required to carry out steps 4.1- 4.10. The detailed specifications and description of the stage may be found in Jun et al.15.
5. Cryo-electron Tomography
6. Three-dimensional Reconstruction using IMOD17
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To characterize the dynamic behavior of the virus particles, HeLa cells infected with HIV-1 were imaged by high-speed confocal live-cell microscopy and the particle movements were analyzed by automated 3D particle tracking (Figure 1). To avoid the time lapse of several minutes that can occur between collection of the last confocal live-cell image and plunge-freezing (which may be long enough to lose correlated HIV-1 particles), a cryo-fluorescence light microscopy stage (Figure 3) was designed and constructed for imaging frozen-hydrated samples15, within cryoEM cartridges, on light microscopes. A copper block platform (Figure 3b, black arrow) and copper ring (Figure 3d, arrowhead) was added for use with non-Polara electron microscopes. Correlation between the confocal live-cell, cryo-fluorescence light microscopy, and cryoET was achieved using indexed gold Quantifoil grids and 0.2 μm fluorescent latex beads (Figure 2). Taken together, Figure 4 demonstrates the feasibility of the overall procedure for advanced correlative live-cell microscopy and cryo-electron tomography to visualize fluorescently labeled HIV-1 particles interacting with a host HeLa cell.
Figure 1. Time-lapse confocal live-cell imaging of HIV-1 particles in HeLa cells. A single green-fluorescent viral particle (yellow arrowhead) was tracked in 3D confocal stacks with a 3 min time interval between frames. Click here to view larger figure.
Figure 2. Correlation between fluorescence images and cryoEM images. (a & b) Differential interference contrast (DIC) images of HeLa cells cultured on a Quantifoil gold EM finder grid, recorded with a 2X (a) and 20X (b) objectives. (c) Cryo-fluorescence image of frozen-hydrated HeLa cells (red color for mitochondria) and 0.2 μm fluorescent latex beads (green), recorded with a cryo-stage and a 40X long working distance air objective, overlaid with a DIC image of cells. (d) Low magnification cryoEM image of the corresponding region in (c). (e & f) Correlation between fluorescence and cryoEM images using 0.2 μm fluorescent latex beads. Corresponding beads are circled with the same color. Scale bars, 200 μm in a, 50 μm in b, 25 μm in c, d & e, 5 μm in f.
Figure 3. Construction of cryo-light microscopy stage. (a) A self-pressurized dewar, filled with liquid nitrogen, used to cool down the copper cryo-sample stage (white arrow). (b) Top, inside view of the cryo-sample stage, showing the inner chamber (white arrowhead). The sample grid is placed onto the EM specimen cartridge, which sits in the center of a copper block (black arrow). Once loaded with the sample grid, the cartridge is transferred to the inner chamber (white arrowhead) for cryo-fluorescence light microscopy. (c & d) The specimen cartridge before (c) and after (d) placing a copper ring (black arrowhead) to keep the grid in place for using with non-Polara cryo-electron microscope.
Figure 4. CryoET of a single fluorescent particle bound to the cellular protrusion of a HeLa cell by correlative microscopy. (a & b) A DIC image recorded with a cryo-light microscopy stage (a) overlaid with a cryo-fluorescence image of GFP-tagged particles (red) (b). (c - e) Low dose cryoEM images of the region containing a fluorescent particle (circled in panel b & c) at 140X (c), 3,500X (d) and 27,500X (e), respectively. Inset, an enlarged view recorded after acquisition of the tomographic tilt series. (f - h) Three 4 nm thick tomographic slices separated by a distance of 21 nm in the z direction. Connections between the particle and HeLa cell membrane are indicated by arrows in both the projection image (inset in panel e) and tomographic slices (panels f & g). Scale bars, 10 μm in a & b, 20 μm in c, 500 nm in d, and 100 nm in e - h.
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We have presented a straightforward set of protocols to provide an advanced correlative approach to analyze dynamic cellular events using time-lapse confocal, live-cell fluorescence imaging followed by cryoET. Our methodological development to correlate 3D live-cell imaging with high-resolution cryoET is critical to investigate many challenging biological problems, such as visualizing rare, dynamic (not static, as previously reported), and diffraction limited viral particles and their interactions with host cells. The spatial and temporal behavior of specific particles in living cells is characterized by live-cell analysis and the structural details of virus-cell interactions at the defined infection stages are explored. A homemade, cryo-light microscopy stage is used to facilitate direct correlation between fluorescence light images from live cells and images captured by cryoEM.
There are several important points to keep in mind in order to have optimal data collection and analysis. First, when picking multiple positions for time-lapse confocal live-cell imaging, the selected positions should be within 0.5 mm from the center of the EM grid, since the edge of the grid interferes with the field of view for tilt-series acquisition in cryoET. Further, the cells selected for analysis should be between two mitosis phases, when they are most spread-out and flat. Therefore, the thin regions of cells, available for cryoET analysis, are at their most extended stage. In order to have the majority of cultured cells at the same interphase stage, cell cycle can be synchronized using double thymidine treatment that blocks cell cycle at the early S-phase18. Cells released from thymidine block then progress synchronously through G2- and mitotic phase.
Second, as mentioned earlier, the time delay between the last frame of confocal image and cryo-fixation of the sample should be minimized to allow efficient direct correlation of dynamic objects. In addition, the flatness and integrity of the grid are critical for all procedures and must be maintained throughout the protocol, particularly when bare grids are handled for non-Polara microscopes.
Finally, precise and reliable correlation between the fluorescence image and the electron microscopy image, to find the target, is required for successful subsequent acquisition of cryoET data. In a projection image, at a low magnification, the recognition of the shape and direction of a selected cell and sometimes counting holes on the grid are necessary to locate the correlated region in the EM field. Fluorescent latex beads are added to the sample for a more robust and accurate correlation. Using fluorescent latex beads or Quantum dots, which are both fluorescent and electron-dense, the coordinates of the target in fluorescence image can be directly transferred to EM for a precise localization. It should be noted that the emission wavelength of fluorescent latex beads or Quantum dots is chosen not to have overlap with the emission wavelength of the target.
Our current correlation set up for identifying a target area takes place outside of a cryo-electron microscope, thus involving an additional sample transfer step from an optical microscope to an electron microscope. A more direct and straightforward method has been thought out19, by installing a fluorescent imaging apparatus within the electron microscope. In addition, the resolution afforded by a long working distance air objective with NA of 0.6 is limited (~0.6 μm). Precise and accurate nanoscale single molecule localization in 3D cellular tomograms will further involve super resolution microscopy to correlate fluorescent labeled proteins in the ultrastructure.
Further advances in correlative light microscopy and cryoET are expected by combining additional techniques, such as cryo-focused ion beam (FIB) milling9,20 and vitreous sectioning8 of frozen-hydrated specimens. These techniques overcome the limitations of the specimen thickness, greatly expanding the range of samples amenable for cryoET analysis, thus allowing investigation of viral particles that have traveled deep inside of the cells. In terms of the study of virus particle interactions with host cell factors, further double labeling of specific cellular protein and the HIV-1 cores with a tetra-Cysteine fluorescence probe fused to HIV-1 CA could facilitate the identification of particular early stages of the HIV-1 life cycle and the spatial and functional relationship of host factors and viral particles. We anticipate the correlative 3D live-cell imaging and cryoET methodology to play important roles in study cell signaling, membrane receptor trafficking, and many other dynamic cellular processes in cell biology.
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The authors declare no competing financial interests.
The authors would like to thank Travis Wheeler and the machine shop at the Department of Cell Biology and Physiology, University of Pittsburgh for construction of the cryo-fluorescence sample stage, Changlu Tao and Cheng Xu at the University of Science and Technology of China for technical assistance, and Dr. Teresa Brosenitsch for critical reading of the manuscript. This work was supported by the National Institutes of Health (GM082251 & GM085043).
|Catalog Number
|DMEM 4.5 g/L Glucose w/ L-Glutamine
|Atlanta Biologicals, Inc. Lawrenceville, GA
|Sigma, St. Louis, MO
|HeLa cell culture on EM grids
|Cell tracker
|Invitrogen Corporation, Carlsbad, CA
|Red CMTPX
|Protein A gold conjugates
|Ted Pella, Inc., Redding, CA
|15 nm diameter
|0.2 μm fluorescent microspheres
|Invitrogen Corporation, Carlsbad, CA
|Yellow-green fluorescent
|Heat-inactivated fetal calf bovine serum
|Invitrogen Corporation, Carlsbad, CA
|Invitrogen Corporation, Carlsbad, CA
|Glass-bottom culture dish
|MatTek Corporation
|Gold quantifoil finder EM grids
|Quantifoil Micro Tools, Jena, Germany
|R2/2 Au NH2 200 mesh
|Invitrogen Corporation, Carlsbad, CA
|Glow-discharge device 100X
|EMS, Hatfield, PA
|Tecnai Polara G2 electron microscope with a Field Emission Gun
|FEI, Hillsboro, OR
|300 keV
|Vitrobot Mark III
|FEI, Hillsboro, OR
|Olympus IX 71 microscope
|Olympus America Inc., Center Valley, PA
|LUCPlanFLN 40X/0.6 NA (2.7-4 mm working distance) objective lens
|Nikon TiE microscope
|Nikon Instruments, Melville, NY
|using a 60X/1.35 NA oil immersion objective lens
|Sweptfield confocal microscope
|Prairie Technologies, Middleton, WI
|Tokai Hit live cell chamber
|Tokyo, Japan
|Cryo-fluorescence sample stage
|Homebuilt by machine shop, see reference 15 for the design.
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Whether you are a high school student looking for information about dual enrollment, a college student currently enrolled in another program, or an industry professional looking to earn your next degree or certificate, there's an adventure waiting for you at ENMU. Find the student description that best applies to you below:
ENMU Branch Campuses
For admission information for our other campuses, visit their websites to learn more:
Still not sure where to start? Contact our Office of Enrollment Services to find out more about requirements, deadlines and to find the online application that's right for you.


text:
The first Trikke Academy took place in September of 2007, in Buellton, California. Since then, people from all over the country and all over the world have been coming to us to spend time getting immersed in all things Trikke. As the training arm of Trikke Tech Inc., our job is to prepare you to become a Certified Trikke Professional:
- Trikke Certified Tech Professional
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Being a Trikke Professional means you have a foundation of information, knowledge, & skills.
What We Do & How We Roll
We specialize in:
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(Events focused on Riding Instruction: From beginner to advanced – the skills one needs to become a more confident, safe, and effective rider / Instructor.)
- Coordinating riding events – Not just specifically for Trikke Academy but for the worldwide carving community
Sign up for our email newsletter… and Get Connected!


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Reviewed By Glenn F (Lees Summit, MO) Reviewed for a 2010 Ford F-150 —3/5/2017 12:05 AM
Nice covers for the price. They aren't covers for someone looking for that expensive look. Had a little trouble installing them on the back seat of my 2010 F150. They also took a little while to arrive.


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There's more than one way to skin a cat. 'course, the cat's not real crazy about any of 'em.
attributed to Jeff Foxworthy
This is a discussion on Some Common Sense Proverbs within the Off Topic & Humor Discussion forums, part of the The Back Porch category; 1. A day without sunshine is like night. 2. On the other hand, you have different fingers. 3. 42.7 percent of all statistics are made ...
1. A day without sunshine is like night.
2. On the other hand, you have different fingers.
3. 42.7 percent of all statistics are made up on the spot.
4. 99 percent of lawyers give the rest a bad name.
5. Remember, half the people you know are below average.
6. He who laughs last, thinks slowest.
7. Depression is merely anger without enthusiasm.
8. The early bird may get the worm, but the second mouse gets the cheese in the trap.
9. Support bacteria. They're the only culture most people have.
10. A clear conscience is usually the sign of a bad memory.
11. Change is inevitable, except from vending machines.
12. If you think nobody cares, try missing a couple of payments.
13. How many of you believe in psycho kinesis? Raise my hand.
14. OK, so what's the speed of dark?
15. When everything is coming your way, you're in the wrong lane.
16. Hard work pays off in the future... Laziness pays off now.
17. How much deeper would the ocean be without sponges?
18. Eagles may soar, but weasels don't get sucked into jet engines.
19. What happens if you get scared half to death, twice?
20. Why do psychics have to ask you your name?
21. Inside every older person is a younger person wondering, 'What the heck happened?'
22. Just remember -- if the world didn't suck, we would all fall off.
23. Light travels faster than sound. That's why some people appear bright until you hear them speak.
24. Life isn't like a box of chocolates. It's more like a jar of jalapeno's. What you do today, might burn your butt tomorrow.
"Democracy is two wolves and a lamb voting on what to have for lunch; Liberty is a well-armed lamb contesting the vote."
-- Benjamin Franklin
There's more than one way to skin a cat. 'course, the cat's not real crazy about any of 'em.
attributed to Jeff Foxworthy
Be wary of strong drink. It can make you shoot at tax collectors - and miss.
The measurement of my finger from the tip to the first joint is 1 inch...depth for planting peas.
The measurement to the second joint is 2 inches...depth for corn.
Return borrowed things in better shape than when you borrowed them.
There are two types of trouble...one is the trouble you knowingly walk into, the other is trouble that just happens...it's important to know the difference.
Walk softly but carry a big stick.
if you have to use said stick, make sure who you use it on, doesn't get up.
Grits is good.
Foul language is a sign of a limited vocabulary
Orion, the Big and Little Dippers.
Everyone is a friend until proven otherwise.
Licorice ferns, huckleberries, nettles, sword ferns.
Tabasco won't kill you even if you eat it by the spoonfull.
Don't watch the clock when you're at work.
Fish can see you if you look over the side of the boat.
Fish can hear you if you talk to loud.
Respect the elders.
Never go to bed angry.
That which does not kill you will hurt like the dickens, but it will make you stronger.
Family is the most important thing on earth.
How to play the guitar, spoons, mouth harp, and water filled bottles.
The true meaning of "Self Made Man"
If you don't know something, go to the library and learn it.
The phrases "I don't know", "I forgot", or "I tried (and failed)" are excuses.
There is a difference between an excuse and a reason, know the difference.
Take care of your apperance...even if it is just a t-shirt and jeans.
The world can change everything about you, except your point of view...unless you allow it to.
David L. McDonald
born 1936-passed 2008
precious father, beloved husband
Bill and Izzie: Proud parents of a soldier.
I thought of you all day today when I was at the zoo.
87% of all home invasions happen in the home.
Don't frisk me, I am the weapon.
Sig Sauer P239 DAK (9mm)
NRA Member & Pistol Instructor
If it walks like a duck, quacks like a duck, swims like a duck then it must be a duck
Drugs are bad mmmkkkay
We say that to our CCH students. No one gets it. YOU GUYS (just as I'd expected) are on the ball though! :)
Don't frisk me, I am the weapon.
Sig Sauer P239 DAK (9mm)
NRA Member & Pistol Instructor
I recited this to my husband who laughed his head off and asked me to repeat it again. did he understand it? -- I think he did...his first language is English after all though my strong accent really makes it hard for him to understand me sometimes
Bill and Izzie: Proud parents of a soldier.
I thought of you all day today when I was at the zoo.


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May 05, 2013 3:00 AM
by JAMES J. NESTOR
Sheriff deputy Jason Palmer fit a new bicycle helmet for Morgan Chichy, 8, Saturday at a bike rodeo on the south campus of Indiana University of Pennsylvania.
Area Girl Scouts were invited to learn about bike safety and to develop their riding skills.
Morgan is with Troop 40829.


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found a new home!
Plenty of my friends are looking
for one too. Find a pet to adopt.
I am already neutered, housetrained, up to date with shots, good with kids, good with dogs, and good with cats.
Estimated Age: 6 months
Estimated Weight: 40 lbs.
Temperament: Sweet, playful, medium energy
Crate or Housetrained?: Working on crate and house training
Good with other dogs or cats?: Yes, plays well with other dogs and is not phased by cats
Good with kids?: Yes
Short Bio:
This adorable little fella is friendly, social and ready to go on lots of new adventures with his forever family. He LOVES people and other dogs, and gets along with cats, too. Deacon is learning to like his crate, and sleeps quietly through the night there. He hasn't had any accidents in the house and appears to be house trained. He likes to spend his inside time lounging at your feet for belly scratches, and his outside time galloping through the grass and sunbathing. Deacon definitely needs daily playtime, but has a nice medium energy overall. He is still learning how to be a "grown up" dog and needs someone who can be helpful, supportive and patient with him.
If you'd like to meet this dog, please fill out an adoption application at http://www.sarocks.org/adopt or visit us on facebook at https://www.facebook.com/sarocksorg.
The $85 adoption fee includes spay/neuter surgery, up-to-date vaccinations, microchip, rabies tag, heartworm test, and 1st month's flea/tick & heartworm preventative.
Adopting an animal from a foster home gives you the best option of finding a pet that matches your lifestyle and home environment, because they are in foster, our foster homes are able to give you more information on their temperament and personality.
Deacon, a San Antonio Labrador Retriever puppy was adopted! Here's how you can benefit by looking at adoption as an option to buying a Labrador Retriever for sale in San Antonio, or seeking a Labrador Retriever puppy from a breeder in San Antonio.


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A Canadian comedian's take on a debauched weekend away in Amsterdam has won this year's £8,000 'if.commedies' prize, the new name for the Perrier award, one of the most highly regarded accolades in comedy.
Phil Nichol won over the judging panel, whose decision was announced last night at the Edinburgh Fringe, with his anecdotal style fused with traditional gags. The Fringe veteran's routine included the gem: 'They say being a hostage is difficult. But I could do that with my hands tied behind my back.'
Nica Burns, the producer of the award, said he 'blew everybody away with an outstanding show'.


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2014 Jeep GC SRT
Rational, irrational, realistic? What is your opinion? Who has done what experience?
Fuel Economy of the 2014 Jeep Grand Cherokee SRT8
Re: 2014 Jeep GC SRT
With an SRT expect lower than what the EPA states.
Re: 2014 Jeep GC SRT
Jeeps aren't necessarily known for fuel efficiency. My 5.7L gets 13-14mpg highway and 8-9mpg city. I'm the original owner and every service has been up to date. EPA numbers were 18 highway and 13 city for mine
Re: 2014 Jeep GC SRT
Depending on your driving habits that is about right. A lot of people who own them really don't care about the MPG though. I know I don't. But, I'm getting around what it is rated with my 2012. Eventually all vehicles will be made to shut off automatically when you are stopped (which I hate). Between the new tranny and it shutting down cylinders when cruising it makes a difference.
Re: 2014 Jeep GC SRT
Remember those people claiming to wait for the 8spd for better gas mileage? Or those who purposely put it off for better gas mileage? Uhmmm, yeah. Adding a couple gears isn't going to save any fuel in a 5200lbs+ vehicle with 470hp. There is zero benefit in real world gas mileage and your wallet between the 5spd and 8spd.
I dont think anyone going out and buying a srt is really that concerned about fuel mileage. If that was a concern theyd probably buy a Prius. I waited for the 8 speed because its faster than the 5 speed Im sure Im not the only one that thought that way. The 5 speed was outdated already.
Re: 2014 Jeep GC SRT
Who cares about the mpg. I get average 10 mpg with my v6 3.7 . I live in brooklyn. So its mostly street and some highway.
Re: 2014 Jeep GC SRT
Facts and figures are always interesting for an engineer!
Re: 2014 Jeep GC SRT
For the last 1.5 years around here and the other site, what I stated was a common statement made about the 8spd. Most all of the conversation about it has been for gas mileage purposes. More-so than it being fast. Which, BTW, in real world I will bet the 8spd is not going to be that much faster. Once again, adding a couple gears isn't going to make the same vehicle, with the same weight, and same HP, that much faster. How many times do you see the first mod to add power/speed, by any car person, to be "change the transmission to more gears?"
Re: 2014 Jeep GC SRT
Actually white 6.4, when the 2012 came out one of the main complaints on here and other sites was that it lacked the 8 speed. The main reason was for the gas mileage. Almost all the posts said, not buying the vehicle for good mileage *but* why not put the 8 speed in so that there is better mileage if Chrysler already has the 8 speed.
Re: 2014 Jeep GC SRT
Yes, regarding the fuel consumption would be a VW Polo announced. :D
But a longer range is also an SRT is not bad, right? :confused:
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Lotus, CA, USA < change city >
DJs (15)
Florists (12)
Cakes (10)
Caterers (7)
Decorations (6)
Bands (6)
Rentals (6)
Coordinators (5)
Wedding Day Beauty (5)
Wedding Fashion (3)
Officiants (3)
Bartenders & Beverages (2)
Invitations (2)
Jewelry (1)
Limos (1)
Ceremony Sites (24)
Reception Sites (19)
See All Vendors >
OUR WEDDINGMay, 2010 in Lotus, ca 95651
"Our wedding at Henningsen Lotus Park was wonderful! We kept things simple for our 200 guests; we had a simple redwood arch with red gladiolas by the river for the ceremony and had the reception on the large upper field. Our reception dinner was a simple buffet with sides from back 40 bbq and roast pigs. We had a lot of kids, so we rented a bounce house & a snow cone maker. It was gorgeous & everyone had a ton of fun in the park! A lot of work, but well worth it!"
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Ceremony: Henningsen Lotus Park
Reception: Henningsen Lotus Park
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- Cakes/Candies Vendor: Sena Cakes
- Honeymoon Vendor: The Big Day Honeymoon Registry
- Caterer: Back Forty Texas BBQ
- Rentals Vendor: Party Royale
- Florist: Camino Flower Shop
- Wedding Fashion Vendor: De La Rosa Bridal
- Ceremony & Reception Venue: Henningsen Lotus Park
Cakes/Candies Vendor:
Sena CakesLotus, CA, United States
"Sena made me an amazing rice crispy treat cake! It was 3 tiers & delicious! She also made us yummy red velvet cupcakes- very reasonably priced, very yummy cake!"
Back Forty Texas BBQCameron Park, CA, United States
"tons of different catering plans from pick up, to deliver, to full service with price options for many budgets, plus delicious food! they made our sides & roast pork, and we had tons of compliments!"
Camino Flower Shop1224 Broadway
Placerville, CA, United States
"located in placerville now, Doddie will help you have gorgeous flowers on any budget, and if you need to make bouquets & arrangements yourself, she will make sure you have all of the supplies & knowledge you need!"
Honeymoon Vendor:
The Big Day Honeymoon Registry919 SW Taylor Street
Suite 200
Portland, OR 97205, USA
"A honeymoon registry seemed like a great idea, but this company is not one I would suggest; they changed formats without notice, so I lost many e-cards; my money was in limbo for awhile, but eventually found. Several guests were charged twice, and several gifts were "lost;" the problems were not resolved until I had them send me their itemized list of transactions and pointed out the discrepancies with my account. They say that your registry will be free if you use their agent, but when you actually try to book with an agent, they don't return your calls, so I used the cruise line & got a much better deal. If you want a honeymoon registry, try somewhere else."
Rentals Vendor:
Party RoyaleLotus, CA, United States
"located in shingle springs on durock road- tables were falling apart, umbrellas had to be duct taped together to be usable, the chairs were the only things that were ok. they are the cheapest, but their products are awful! spend the extra money & get tables that will not embarass you!"
Wedding Fashion Vendor:
De La Rosa Bridal319 East Bidwell Street
Folsom, CA, United States
"the only reason I did not buy my gown from them is that they do not carry the designer I chose. They did do an amazing job on my alterations though, and I bought an amazing veil. I tried several gowns there & was surprised at their selection, and their sale rack! You have to check it out! Tons of gorgeous reasonably priced gowns!"
Ceremony & Reception Venue:
Henningsen Lotus ParkLotus, California, United States
"the parks system was easy to work with, accomadating, and the park is so well maintained it made the perfect gorgeous backdrop for our ceremony & reception. Marshall park is also excellent- don't be afraid of a park wedding for a large group- we had 200 guests & were planning for 250- it was awesome!"


text:
R.B. Woodward Lecture on Vitamin B12 Synthesis
March 11th 2016
When I was a post doc at Harvard one of the real treasures was the chemistry library, which had some very impressive archives as you might imagine. Amongst these was a DVD recording of a lecture that R.B. Woodward gave in 1972 following his completion of the total synthesis of vitamin B12 (along with Albert Eschenmoser). If you are into this sort of thing and have 3.5 hours to spare it is a real gem. Here is part 1:
And part 2:


text:
Free personalized radio that
plays the music you love
U Got A Problem?
Come see this ol' light-skinned motherf**ker
I seen him and I'm addicted
He serving a piece of the click
Features of This Tracksouthern rap roots
danceable beats
use of call-and-response vocals
duo rapping
explicit lyrics
a slow moving bass line
mellow piano timbre
subtle use of pianos
synth riffs
a highly synthetic sonority
trippy soundscapes
radio friendly stylings
lyrics by a rap icon
production by a famous producer
and many other similarities identified in the Music Genome Project
These are just a few of the hundreds of attributes cataloged for this track by the Music Genome Project.show more
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text:
BAGHDAD, (Xinhua): A total of 51 people were killed and 191 others wounded in violent attacks across Iraq on Sunday, mostly in the capital Baghdad, police said.
Five people were killed and 18 others injured when a car bomb exploded Sunday evening in the Aalam neighborhood of southwestern Baghdad, a police source told Xinhua.
Three people were killed and 16 others wounded when a car bomb went off in a popular market in the Jamela area in eastern Baghdad, the source said, adding that one civilian was killed and five others were injured when another car bomb exploded in the Talbiya area of eastern Baghdad.
Earlier in the day, a total of 13 bombing attacks predominantly ripped through Shiite districts in Baghdad, killing at least 38 people and wounding some 146 others.
In Iraq's eastern province of Diyala, a civilian was killed and six were wounded in a car bomb explosion near a restaurant in the town of Jadidat al-Shat, some 30 km northwest of the provincial capital city of Baquba, which is about 65 km northeast of Baghdad, a provincial police source said.
Near Baquba, gunmen shot dead Ahmed al-Khafaji, head of the provincial media office for the leading Shiite party of the Islamic Supreme Council of Iraq (ISCI), headed by Shiite cleric Ammar al-Hakim, the source said.
Also in Diyala, two more people were shot dead by gunmen in two incidents near Baquba during the day, the source added.
No group has so far claimed responsibility for the deadly attacks, but the al-Qaida front in Iraq, in most cases, were responsible for suicide bombings in the country.
Sunday's attacks came amid escalation of sectarian tension between the Sunni and Shiite communities, which has been at its highest level since the U.S. troops pulled out from the country at the end of 2011.
Iraq is witnessing its worst eruption of violence in recent years. According to the UN Assistance Mission for Iraq, 8,109 Iraqis were killed in the country from January to November 2013.


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Christmas Season Anger Management Stories
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December 22nd, 2008, 05:28 PM
Silver Bullet
Livin the American Dream
Join Date: 04-18-07
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Christmas Season Anger Management Stories
So Saturday night my wife and I were at Meijers at 96 and Middlebelt. We were the only people waiting in line for the self check out. A black older women pulls her cart up next to us and we chat while waiting. I did not realize that 2 lines of approx 20 people had formed behind us. someone finishes up and the older women gives me the nod to go ahead. I start walking toward the check out and I hear from the back of the line from a black couple " O hells naw, How he gonna cut up in front of dat der lady. Dis here is some bull shit. Hey mans hows the hell you gonna do dat to us." I turned around and said "I'm sorry I can't understand you I don't speak ebonix."
That set his fat ass wife off. "O hells naw I cants beleives he just said dat." Picture her arms flopping around like she is trying to fly. "WTF is dat supposed to means." A few of the people in line were trying not to laugh. The person running the self check out was almost rolling on the floor. My wife was laughing her ass off but smacked me when we got in the car. The whole time they were flappin at the jaw i was laughing my ass off and I waved good bye as I left.
What kind of stories do you got from the "season to be jolly."
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