text:
One of the biggest issues empty nesters face when they downsize from a single family home is space.
Suffice it to say, it's not a simple task to make the transition from a 2,500-square-foot home (including perhaps another 1,500 square feet downstairs) to, say, a 1,400-sq.-ft. apartment-style condominium. Issues almost always arise with storage space (i.e., a lack thereof with no basement) and living space (not enough).
Then, there's the fact there's no double garage to park the cars in -- and little or no green space to enjoy while entertaining.
Thankfully, Longboat Development Corp. has teamed with Irwin Homes to create Country Estates Condominiums, a development that will feature 36 bungalow and two-storey condominiums on a pristine parcel of land in The Oaks.
"Country Estates offers bungalow, two-storey and semi-detached units ranging from 1,391 sq. ft. up to 1,819 sq. ft.," said Longboat Development Corp.'s Ryder Needham. "It's a great location nestled away in a preserved urban forest just outside the Perimeter Highway, yet it's still within city limits. It offers a breathtaking, natural environment that's only minutes from city conveniences."
Turns out, the condominiums are as impressive as the countrified setting that surrounds them. One of the most notable aspects of their interior design is the fact the layout makes units feel larger than their listed square footage.
Needham said while the display suite is 1,395 sq. ft. in size, it feels significantly larger.
"The builders, Irwin Homes, did an exceptional job with the interior layout," he said. "It features an open-concept floor plan, as do all the units. "There's a den/bedroom to the right of the (approximately seven-foot by eight-foot) foyer, and then there's a four-piece bath on the same side as the den/bedroom that's only steps away; there's also a main-floor laundry/mudroom (just before the great room to the left), too. Everything makes sense -- you feel like everything has been placed exactly where it should be."
From there, the foyer, which is defined by rich, textured taupe tile, merges into a great room that's defined by space and light. Even though the main floor is only 1,395 sq. ft., every inch of the space is well-used and well-lit.
"Even though the great room is an open-concept design, which makes for excellent flow from space to space, each space is defined," said Needham. "The kitchen features an eight-foot by four-foot island with (beige/taupe) quartz countertop, undermounted double sink and breakfast bar for three. There's plenty of room to move around the island and in the kitchen, making it an area that's ideal for cooking and entertaining."
Finishes in the kitchen are impressive: countertops with more of the quartz that tops the island; espresso maple cabinets (loads of them); an off-white subway tile backsplash; and dark wide plank laminate hardwoods that run through the kitchen and dinette areas. A six-door pantry adds a practical touch, with plenty of storage space for key culinary items.
Meanwhile, the rear portion of the kitchen is inhabited by an oversized dinette area that comfortably seats six -- plus a patio door and huge window.
"The patio door leads to a backyard deck that's great to grill on and sit outside with guests when you entertain, while the window lets in all kinds of natural light. One of the best features of the great room, aside from all the space it offers, is that it's filled with so much natural light. The unit's rear wall is filled with glass, including a huge three-section picture window in the living room."
Just as the kitchen is defined by its island and the dinette is defined by a jog in the wall between it and the living room, the living room is stylishly defined by a tray ceiling and beige berber carpet. The result is a great room that feels well-balanced, with plenty of light, space and subtle separation.
"It's another example of just how much time Irwin Homes' design team put into the floor plan," he said. "They got every detail just right, making for a very livable, versatile area."
Another space that's eminently livable is the home's master suite, which, like the great room, is bright, spacious and well-appointed.
"It was placed away from everything to make it a space that offered as much privacy as possible. With its high ceiling and generous size, there's plenty of room to work with, while a huge picture window lets in all kinds of light. It also comes with a huge walk-in closet (with built-in storage), as well as a three-piece ensuite with five-foot shower and white maple vanity with grey countertop and oval sink."
Two more design features make the unit -- which feels more like a single family home -- an ideal residence for active empty nesters to downsize into.
"Storage is often a concern for empty nesters, but it isn't here," said Needham. "You get a double attached garage, plus a basement that offers about 1,200 sq. ft. of livable space. You can put a workshop in the garage and use the basement in whatever way that best suits your needs: for storage, or you can put in a rec room, two bedrooms, a four-piece bath and storage room. The bungalow and two-storey units in Country Estates Condominiums have the space, style and convenient location that perfectly suits the needs of empty nesters."


text:
Helen Gurley Brown, editor in chief of Cosmopolitan magazines' 64 international editions and one of the world's most influential editors, died Monday at New York-Presbyterian/Columbia University Medical Center. She was 90-years-old.
Brown had an enormous influence on popular culture and society, which first began with her best-selling book "Sex and the Single Girl" in the early 1960s, and later with her almost forty decade tenure as Editor of Cosmopolitan magazine.
For more about the life of Helen Gurley Brown, Click here.

subset: commoncrawl


text:
Attachment 86686
Printable View
Attachment 86686
Reminds me of an Acura TL
Is there a difference between the two pics above?
The exhaust tips.
That's not a picture or rendering of a V. That's the 2.0T.
Yeah, the title is kinda bad. It's just a rendering of what the ATS-V might look like from the back (without Cadillac's V-Series badge).
Expect bigger outlets, and some serious lower fascia changes.
GM always dresses up the lower fascias on SS/V-Series type cars.
I like 'em outboard myself.
There is a guy in the V forum who just saw an ATS-V at the dealer. I think we are missing out here. Find that car Sal!
That ghai was on crack.
I like my tips out toward the edges. Leave the inward poo shoots to the vettes I say.
^here here


text:
|Thread Tools
I know how to sail, but I don't know many of the actual rules or principles. Can you help?
Mark Matthews responds:
There is a lot of mystery over how sailboats work since the medium they use, the wind, isn't directly observable. Basically a sail is nothing more than a vertical airplane wing, or foil, that can be adjusted. Sailboats are actually pulled through the water on most points of sail by a force known as lift. A sailboat can't sail directly into the wind. If you point a sail directly into the wind, it flaps around-much like a flag on a flag pole will. If you head the boat away from the direction of the wind by about 45 degrees, however, and sheet or pull the sails all the way in, the boat starts moving. What is happening is that the air molecules are divided up by the sail, which is foil shaped. Air flows a greater distance on the outside of the sail than it does on the inside of the sail. The air on the outside of the sail accelerates to meet up with the air on the inside of the sail. This creates a difference in air pressure which causes lift and pulls the boat forward. Interestingly, similar forces are at work beneath the water as well. The keel or centerboard is like a sail in the water, and while it may also provide ballast for the boat, it converts the forces at work into forward momentum. Without the underwater resistance, the boat would just go sideways.
So how does a sailboat travel to a point directly up wind? Basically, it takes a lot of tacks as it zigs and zags its way upwind. A general guideline for sail trim is that when pointed as close to the wind as possible, the sails are trimmed in all the way. When going dead downwind, or with the wind behind, the sails are let all the way out. On this point of sail, the boat is no longer being pulled by the wind, but is actually being pushed. When the wind is perpendicular to the boat, the sails are half-way in, half-way out.
I'd refer you to some other articles on our site which will be able to provide you with more information. Look, for example, in ourLearning to Sail Collectionspecifically I'd recommend taking a look at Steve Colgate's articles.
|Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
|Thread Tools
Show Printable Version Show Printable Version
Email this Page Email this Page
|Posting Rules
You may not post new threads
You may post replies
You may not post attachments
You may not edit your posts
BB code is Off
Smilies are On
[IMG] code is Off
HTML code is On
Trackbacks are On
Pingbacks are On
Refbacks are On


text:
The Essays of Brother Anonymous
Ascension and the Coming Golden Age
Last revised: Aug. 29, 2009.
Perhaps the best starting point for a discussion of Ascension as part of a Divine Plan for humanity is the recognition that, as Matthew Ward says, "God rules all major decisions in this universe." (1) The galactics agree. Says Atmos of Sirius: "The Creator … has the last word and it is absolute Law." (2)
In my opinion, all the ascended masters, galactics, and celestials leading the preparations for 2012 would undoubtedly sign on to these statements as being the bedrock of their outlook.
SaLuSa of Sirius suggests that we terrestrials require a deeper understanding if we are to take full advantage of the Ascension opportunity before us. "A deeper understanding that will lift you higher … must really start with the belief in God, although Ascension can be seen simply as a normal process. With a greater understanding you will realise that there has always been a Divine Plan for your evolution and what you are about to experience is part of it." (3)
According to Atmos of Sirius, "the planning for Ascension started long ago." (4) The spirit guide Saul says it has been in the works "for eons." (5) "From the commencement of the last cycle over 12,000 years ago, it was known that it would be completed through the process of Ascension," SaLuSa says. (6)
In Matthew's view, the events that would occur at this time were "anticipated by the highest universal council members who long ago conceived and crafted the Golden Age's master plan.
"The plan included millions and millions of souls from advanced civilizations whose essential assistance on, within and above Earth assured that [the planet] not only would survive death throes, but would be restored to her original paradise self, where all her people live in harmony with each other and all of Nature." (7)
The primary local authority in the Ascension operation is the Earth's own spiritual hierarchy. St. Germain speaks on their behalf: "God has decreed that the end times should bring you the opportunity of Ascension, so look into your heart and see if you are ready to release your lower vibrations." (8)
The spiritual hierarchy reaches upwards to God and directs God's commands down through dedicated councils and federations.
According to Matthew, commands come "directly from God" (9) while the "grand plan being worked out [is] supported by great Beings of Light." (10) These "Higher Beings … have assessed the state of Mankind's level of consciousness." (11) Apparently, their plan takes all details into account.
To assist it in its work, the local spiritual hierarchy turned to the Galactic Federation, who brought their higher technology and helped rid the planet of a dark cabal controlling it.
Speaking for their membership, SaLuSa asserts that "we of the Galactic Federation are on a divine mission." (12) He explains: "The Creator has expressed a desire that all within your Universe shall ascend and it is only your Earth that is experiencing a battle to be part of that upliftment. All other planets and dimensions are sufficiently advanced, to move with the changes without any difficulty." (13)
Of the divine intent, he says: "Your All-Loving God has plans well beyond your understanding and the goal is to successfully move your Universe into the higher vibrations. That process is well advanced, and you and your Earth and being prepared to be part of that upliftment." (14)
He reminds us that "you must know by now we are not here in great numbers by chance. It is to fulfil the Creator's Plan for this Universe. Nothing can prevent it manifesting in Ascension." (15)
Who are the galactics? "The Galactic Federation comprises those civilisations that are of the higher vibrations, having long passed through a similar period of evolution to yours. …
"Nevertheless, we have always been near enough to you to assist in ways that have not always been obvious. We now await our call to announce First Contact, and it is an exciting prospect for us after all of this time. We are absolutely ready for immediate action, and have fully equipped ourselves for the multi-tasks that lie ahead." (16)
According to Sheldan Nidle, "we are dedicated to moving you to full consciousness…. What you are currently going through is the divinely-devised process to free you and help you re-establish the inner and outer responsibilities which go with your rise in consciousness." (17) This "full" consciousness is unitive consciousness.
There is no division between the galactics and the local spiritual hierarchy. Says Diane of Sirius: "We can tell you that the highest Orders and Councils are one in a Brotherhood of Light." (18)
United and serving God and humanity, the galactics and the hierarchy allege that their success is "inevitable," (19) "a certainty." (20)
"Heaven is unfolding [the] Divine Plan" for Ascension. (21) The cycle of duality will end "as decreed." (22) The outcome is "pre-ordained." (23)
"Nothing can take that opportunity away from you," SaLuSa states. (24) "Ascension … will come regardless of how many stops you make on the way." (25)
Ag-Agria explains:
"Your destiny is absolutely guaranteed and nothing can stop your Ascension. You will understand why regardless of what is yet to happen on Earth, it is not going to prevent the outworking of all that has been planned for you. Go with the flow and adjust as necessary, because you do not have much longer before you will be part of the changes that are so eagerly sought.
"Allow the old to crumble into disuse as it has served its purpose now, and let those who still cling to it create their own pathway through their freewill choice." (26)
Sheldan Nidle suggests the buoyant mood reigning among the combined Ascension forces pondering coming events:
"Heaven is in joy that what she is doing will soon end your long period of unbroken amnesia. You are blessed by Heaven, and therefore the great shift that she is creating is inevitable." (27)
The Divine Plan has within it certain dates by which each event must happen. The 2012 scenario happens according to "the boundaries set by the Divine." (28) "[God] has His own timetable" for Ascension with "specified date[s]." (29) The events will occur "precisely on schedule," though the need for secrecy prevents them sharing that schedule. (30)
Prior to the divine deadlines, we "are experiencing life on a freewill planet." This means that "beyond instructions given us by the Higher Councils, our presence [cannot be] intrusive." (31)
However, if by any chance a specified event has not commenced by the divine deadline, "there will be intervention on our part to ensure it can go ahead. That has been divinely decreed and is our authorisation to do so." (32)
SaLuSa relates that the galactics have extended their help in the past and have been rebuffed. "On a number of occasions we have tried to offer our help, and it has been turned down.
"However, we no longer … need … your permission to arrive on Earth, as it has become a divine order with an ultimate date by which we will appear. We will do so in a perfectly correct manner and first approach those who are your legally-appointed representatives." (33)
Once a divine deadline is passed, the free-will dimension of the planet is suspended and the Divine Plan takes precedence over our free-will desires.
(To be continued)
(1) Matthew's Message," Aug. 21, 2009 at http://www.matthewbooks.com/mm/anmviewer.asp?a=105&z=2.
(2) Ag-agria of Sirius, Feb.16, 2009 at http://www.treeofthegoldenlight.com/First_Contact/Channeled_Messages_by_Mike_Quinsey.htm.
(3) SaLuSa of Sirius, Aug. 21, 2009, at http://www.treeofthegoldenlight.com/First_Contact/Channeled_Messages_by_Mike_Quinsey.htm. "Your belief in God lifts your consciousness to a level where the truth can open up to you, where you 'allow' the immutable link that already exists to enlighten you. Once this step is made your Higher Self is able to come more into your life, guiding you and ensuring the completion of your life plan."
(4) Atmos of Sirius, Dec. 17, 2008, at http://www.treeofthegoldenlight.com/First_Contact/Channeled_Messages_by_Mike_Quinsey.htm. xx (5) Saul, "You are absolutely entitled to this inheritance," through John Smallman, July 1, 2009, at http://johnsmallman.wordpress.com/.
(6) SaLuSa of Sirius, April 3, 2009, ibid.
(7) Matthew's Message, Aug. 21, 2009, ibid.
(8) St. Germain, Update, June 25, 2008, through Mike Quinsey, at http://www.treeofthegoldenlight.com/First_Contact/Channeled_Messages_by_Mike_Quinsey.htm. xx (9) Matthew's Message," Aug. 21, 2009 at http://www.matthewbooks.com/mm/anmviewer.asp?a=105&z=2.
(10) SaLuSa of Sirius, April 1, 2009, ibid.
(11) SaLuSa of Sirius, May 27, 2009, ibid.
(12) SaLuSa of Sirius, April 8, 2009, ibid.
(13) SaLuSa of Sirius, April 8, 2009, ibid.
(14) SaLuSa of Sirius, April 6, 2009, ibid.
(15) SaLuSa of Sirius, March 30, 2009, ibid.
(16) SaLuSa of Sirius, Dec. 15, 2008, ibid. I'm aware of George Kavassilas's warnings about the crocodilian reptilians posing as the Galactic Federation, but do not share his alarm over the situation, which I think the true GF either has taken care or will take care of.
(17) SHGF, "Update," 2 Dec. 2008, ibid.
(18) Diane of Sirius, Feb. 2, 2009, at http://www.treeofthegoldenlight.com/First_Contact/Channeled_Messages_by_Mike_Quinsey.htm.
(19) SHGF, "Update," Sept. 2, 2008 ibid.
(20) SaLuSa, June 29, 2009, ibid.
(21) SHGF, "Update," 27 Jan. 2009, ibid.
(22) SaLuSa of Sirius, Oct. 20, 2008, ibid.
(23) Ag-Agria, Feb.16, 2009, ibid.
(24) SaLuSa of Sirius, May 27, 2009, ibid.
(25) SaLuSa of Sirius, Feb. 23, 2009, ibid.
(26) Ag-Agria, Sept. 1, 2008, ibid.
(27) SHGF, "Update," Sept. 2, 2008, ibid.
(28) SHGF, "Update," Feb. 17, 2009, ibid.
(29) Matthew's Message," Aug. 21, 2009, ibid.; SaLuSa, March 23, 2009, ibid.
(30) Saul, "The coming changes that have been divinely decreed will occur precisely on schedule," May 31, 2009, ibid.
(31) Ker-On of Venus, Oct. 22, 2008, at http://www.treeofthegoldenlight.com/First_Contact/Channeled_Messages_by_Mike_Quinsey.htm.)
(32) SaLuSa of Sirius, March 23, 2009, ibid.
(33) SaLuSa of Sirius, February 18, 2009 ibid.
The Essays of Brother Anonymous
Ascension and the Coming Golden Age


text:
This Friday, the City�s Department of Transportation, Traffic and Parking will be releasing the Smart Cycling Handbook at the Bike to Work Breakfast held at Devil�s Gear Bike Shop, 151 Orange Street. The breakfast will be held from 7:30am to 9:30am and copies of the Smart Cycling Handbook will be available for everyone who signs a Smart Cycling Pledge.
Here�s what else is happening in New Haven:
The Health Equity Alliance and Health Matters New Haven will be hosting a Lunch and Learn Series. The series, titled Unnatural Causes, explores racial and social inequalities in health. All showings are from 11:30 to 1 p.m. on Wednesdays in Meeting Rooms 1 and 2 on the second floor of City Hall, 165 Church Street. Lunch will be provided. Space is limited and RSVPs are necessary. RSVP to Shanta L. Evans at firstname.lastname@example.com or call (860) 869-8779. Dates are as follows:
February 2: �In Sickness and Wealth�: Part 1
How does the distribution of power, wealth and resources shape opportunities for health?
February 9: �In Sickness and Wealth�: Part 2
How does the distribution of power, wealth and resources shape opportunities for health? Location to be announced.
February 16: �When the Bough Breaks�
Can racism become embedded in the body affect birth outcomes?
February 23: �Becoming American�
Latino immigrants arrive healthy, so why don�t they stay that way?
March 2: �Bad Sugar�
What are the connections between diabetes, oppression, and empowerment in two Native American communities?
March 9: �Place Matters�
Why is your street address a strong predictor of your health?
March 16: �Collateral Damage�
How do Marshall Islanders pay for globalization and U.S. military policy with their health?
March 23: �Not Just a Paycheck�
Why do layoffs take such a huge toll in Michigan but cause hardly a ripple in Sweden?
Health Matters
Visioning a Healthier New Haven conference will be held on Thursday, January 27th from 8.30am till 2pm at the New Haven Lawn Club. For more info visit: www.healthmattersnh.org.
Green Tip
Want to save on your home energy bill? Sign up for a FREE energy assessment for your home through the Office of Sustainability. Average savings are $500 over two years. See http://www.cityofnewhaven.com/Sustainability/GHHI/Preview/ for more details.
The following cultural events are taking place in the City this week:
Jan. 19. Honey Dewdrops in Concert. Main Library. 6:30-7:30pm.
Jan. 20. Dance in Dialogue. Artspace. $15/20 at door. 8pm. www.rachelbernsen.com
Jan 21.Yale Philharmonia. Yale School of Music Woolsey Hall. 8pm. http://music.yale.edu/concerts
Jan. 22. Yale Schola Cantorum: Music of the High Cathedral. Christ Church Episcopal. www.yale.edu/ism/events.
Jan. 23. Edgewood Park Farmers Market. 10am-1pm. www.cityseed.org.
I hope you have a happy and healthy week!
Very truly yours,
John DeStefano, Jr.
Contact Name: Jessica Mayorga
Contact Email: email@example.com


text:
/// Blockbuster iPhone Sales and Flipboard's New Round: AllThingsD's Week in Review
Apple The 5c iPhone comes in green, blue, yellow and pink, as well as white. Another busy week in the digital world has passed, complete with our full coverage. Here's a download of the top news this week, in case you missed it: Despite concerns from analysts and pundits, Apple managed to sell a whopping nine million new iPhones over the first weekend of release. Those numbers, however, weren't broken out in terms of how many were 5s devices versus 5c — perhaps we'll hear more on the quarterly earnings call. The NYPD would really love it if all Apple mobile device users — especially those buying new iPhones — would download iOS 7. The new software comes with added security features, which could help deter theft. Flipboard, the social magazine startup, raised a hefty $50 million in a round led by Rizvi Traverse Management and Goldman Sachs. The company seems to be growing steadily, too; at last count, CEO Mike McCue said Flipboard has more than 85 million users. It was round two for Microsoft's Surface tablets, as the company released the next generation of its mobile computing devices. You need to read Peter Kafka's hilarious account of the launch . IHS tore apart the guts of the latest iPhone releases to figure out how much it costs Apple to build them. The result? Around $200 a pop — not entirely different than the last generation.
Read the rest here:
Blockbuster iPhone Sales and Flipboard's New Round: AllThingsD's Week in Review
Related Posts
- 05/25/2017 • Publishers Creating Facebook Instant Articles Can Now Simultaneously Work on Google AMP (Apple News Soon)
- 04/10/2017 • Daily Download: Five Stories You Need to Read Today
- 04/04/2017 • Gerry Graf Picks His 3 Favorite Ads, Including One for E*Trade That Isn't the Dancing Chimp
- 04/03/2017 • AI vs. Chatbots
Leave a Reply
You must be logged in to post a comment.


text:
Snowflake Pumice Stone
If you are going to scrub your feet - the scrubber may as well be fabulous right??? This adorable snowflake pumice is the perfect stocking stuffer.
LOVE IT!! Keep this by the sink in the guest bathroom....smells great and looks pretty too.Tags: beauty, lotions, bath & Body, holiday gifts, holiday shoppingAdded 10 Years Ago from Bath and Body Works


text:
Self Ease
Self Ease take the perfect selfie everytime! Self Ease as seen on TV is the telescoping pole that allows you to fit all your friends and that amazing background in the shot. Telescoping pole extends up to 40 inches! Collapses to just 7 inches for easy storage. Self Ease securely holds all major smartphones and digital cameras. Never bother a stranger to take your photo again. And Self Ease is perfect for shooting video above crowds to capture those special moments. Self Ease is easy to use just attach your smartphone or camera, extend the pole and take the perfect selfie.
Availability: In Stock
Ready to ship in 1-2 Business Days
Reviewed by 2 customers
- Easy To Set Up
- Easy To Use
- Low Power Consumption
- Quality Construction
Best Uses
Comments about this product:
These were purchased as gifts for my grand-children ages 15, 16, 17, 21 and 24 and they absolutely love them!!! I am now the coolist Nana ever! Easy follow directions and worked great right out of the package. Love seeing their post using their "selfie" photos!
- Primary use:
- Personal
- Was this a gift?:
- Yes
- No remote
Best Uses
Comments about this product:
It's cool and I can touch the ceiling with it!
- Primary use:
- Personal
- Was this a gift?:
- Yes


text:
It's been a while since I've shared a photo of Little Afrobella, AKA my niece Dominique. And I've shared even fewer of her sister, my even littler niece Isabella. So here's their official Afrobella reintroduction! Are they not ADORABLE? As you can imagine, it takes quite a bit of patience and love to get their… read all
I already wrote my Gift Guide for Little Girls, so I really wasn't planning on featuring more gift items for kids here on Afrobella this week…then last night I had a delightful surprise at Whole Foods here in Chicago. There I was, minding my own business and who do I run into but the Double… read all
Yes of course, grown folks love Christmas too. But there's no denying the fact that Christmas is for children. It's all about seeing those little faces light up, after the perfect present has been opened. I have scoured the internet to find five wonderful gift items for the little afrobellas in your life. Click to… read all


text:
|Topic Review (Newest First)
We both used names in the business to prove our point.That in these forums is inappropriate.The advise we both prove should be based on trust of what we both know and not use names.
Dirty hands experience for me started way back as a engine tester/dyno rm tech working at the Chevy engine plant in Tonn,NY under the UAW 774.I started a learning curve under the supervision of Frank Leuendowski and Gorgie Shears.Before that under the guidance of a world war II motor pool mechanic Paul Weaver.At 65 yrs old I have spend my entire life as a study in dirty hands.I have been involved in a partnership of a very successful professional race operation of over 40yrs where 20yrs of it was in 9.90 racing winning a national event and currently a TAD with nitrous that ran in the 1/8 firstname.lastname@example.com your off base in your comments.We have been involved as a business for the whole time in the partnership in sales of overstock and recondition high performance parts where it was a tradition for three yrs to have dinner with the industry leaders because of our purchases we made.
Sir as always there is two sides to a story.That has been the case about long rod vs short rods since Smoky.So moving forward given our backgrounds it should be enough when we say it is like this.Even if we don't agree about a topic,that is why forums like this one works.
I don't think in this whole exchange at anytime I disrespected you.And Richard is a friend.(Techinspector 1) who I have know for awhile.I certainly respect his knowledge that time and again he has proven in posts on two forums.But I do disagree with him from time to time.
I am going to wrap up my posts on this topic like this.We all know and believe to stroke a engine adds to it's power output.Right??. Then to add to the length of that stroke with longer rods at the very least,a mechanical lever advantage should be a given. Really a simplistic answer??. Is it more complicated than that??. Yes it is.But that debate is for another thread sometime later.
To the O/P of this thread.I sincerely apologize how this got out of hand.
This discussion of long rods vs short has gone on for yrs.And will go on long after we stop talking about it.
I done with it.
Ron is posing that other side of the discussion.Just because it's Ron doesn't mean he is right.Don't exclude all the references I posted that believe a long rod is worth while.And then I am telling you it is.
We needed to swap out a 5.7 engine for a spare identical short block 6" rod.Actually used the same cam.That 6" rod engine would put on a fender consistently at the top end.Sensitive butt dyno,it just felt faster.We knew John at LPE and I am very sure he believed the worth of long rods.
What Howards explained to me was they are tired of complaints about thrust side piston wear on there 3.75 stroke 5.7 rod cranks and have stopped selling the cranks by themselves because of that.
And I do not subscribe to the 6" rod use for the street because of the ring spacers.That s why I was very happy to get the last Howards 5.7 rod crank you see in the picture.
I'll weigh in here with some thoughts....
A 400 motor will be fine on the drag strip without steam holes being drilled in the heads. On the street, where the motor will be run south of 3,000 rpm's and idle for periods of time, it is likely to create hot spots and spit out the head gasket. Above 2,500/3,000 rpm's, there is enough turbulence created in the water jackets that steam pockets cannot form.
Hopefully, understanding the rules and using a modicum of common sense will allow a racer to pass tech with a front-mounted fuel cell. The idea is to get it inside body lines on a doorslammer and hopefully behind a chassis crossmember. Altereds and diggers need further protection. Here's the rule.....
"Tanks: When permitted by class regulations, fuel tanks located outside
body and/or frame must be enclosed in a steel tube frame constructed
of minimum 1 1/4-inch O.D. x .058 chromoly or .118 mild steel tubing.
All fuel tanks must be isolated from the driver's compartment by a
firewall, completely sealed to prevent any fuel from entering the driver's
compartment. All fuel tanks must have a pressure cap and be vented
outside of body. A positive-locking screw-on fuel tank cap is mandatory
on all open-bodied cars. Insulated fuel tanks prohibited. When used,
fuel cells must have a metal box protecting the part of the fuel cell that
is outside of body lines or trunk floor, excluding hose connection area
in rear. The metal box must be constructed of minimum .024 steel or
.032 aluminum. Nonmetallic fuel cells or tanks must be grounded to
This last part of the rule probably makes no sense to some of you who have not run cells, like how do you ground plastic, but what it refers to is grounding the metal ring at the fill hole of the cell. Use a piece of 10 ga wire and crimp connectors. Any frame crossmember that is forward of the cell will work in place of the tubing protection called for in the rule, even on an open car. Take a look at any digger. All Funny Cars have front-mounted cells too. We used to be tough on firewalls. Find a very dark place, position yourself so that you can see the firewall from inside the car and have a buddy shine a very strong light on the firewall from the engine compartment. Everywhere light can shine through is a place where fuel could pass through to the driver's compartment. If that fuel was on fire, well, you get the idea. Small holes can be filled by using a fender washer on each side of the firewall with a bolt and locking nut through the middle of the washers. Larger holes will need to be welded up with patches.
Whenever the question of rod length comes up, I always refer to the articles published by Ron Iskenderian, son of the famous camfather, Ed Iskenderian......
Please read Tech Tip 2005 here....
ISKY Racing Cams - Do It Right. Race with the Legend. Camshafts, Connecting Rods, Valve Springs, Lifters
Smokey Yunick,LPE,Howards,all aftermarket blocks,GM it's self all disagree with you on the long rod question.
I still,even through I have gotten older,have the ability to listen and learn.Some guys get hard of hearing and are tough to teach.Oh well.....................maybe there is something for them still living "back in the day".
[QUOTE=hcompton;1618056]Under hood tanks go in the front in same location as battery or coolant tank.
That's where things get questionable. The rule say's... "Tank must be mounted within the frame".
If it was mounted in the battery location that would "not be within the frame" and if the car took a hit in the front the fuel tank would be in for a good hit.
|Still doing the dance avoiding the commitment I challenged you to make......
That cert covers nation wide,not just in DC.
Still doing the dance avoiding the commitment I challenged you to make......
A lower flash point catches fire sooner/faster.If that wasn't the case,there would be no point in running 110 race gas.A higher flash point requires more heat like the case for kerosene.Diesel fuel has a higher flash point and what makes that work is in diesel engines the extreme high compression.So you have the flash points backwards.
|For the record.Race gas's flash point is lower than pump gas.I am very sure about that being a dangerous goods specialist register in Washington,DC.
|But to establish a rock solid ref from this point moving forward,I believe with at 65 yrs old of a lifetime in a study in performance engines the 6" rods do have a positive effect.
Well it's like a cliff being sold because everyone else jumps off it,refills under the hood tanks by hot headers needs to be with full fire suits only or else it's a adventure.I demand anyone advising their use posts they will go to the burn unit to visit anyone anywhere if something goes wrong.
Howards stop building 5.7 cranks where you could buy the crank by it's self mainly because the 5.7 rod creates too much rocking of the piston's thrust side and that creates excessive wear.I got the very last one is why I know.I bought it as a trade off because I don't like ring spacers that 6" rods need for the street.What all of us do is build things into a engine build that adds alittle bit and all those things adds up to a final output.The topic of long rods goes back to the days of Henry"Smokey"Yunick saying "you put in the longest damm rod you can find",followed by John Lingenfelter at LPE gradually over the yrs increase the rods they use in their engines,to GM Chevy it's self increasing the rod length in it's production engines.It is well documented the positive effect a 6" rod and greater than that has.It is also follow through by the aftermarket blocks being offered.
When we used a OEM 400 we would fill the block 1/2 way to help stabilize the bores and bore and hone using torque plates.Even then the bores sealing ability would result in a different leak down test everytime.And we where running them in a N/A 9.90 class.No nitrous.
That was yrs ago and the good 400 cores where getting harder to find. You say you have a 400.Well sonic check it even with only a .030 over bore to check it for a possible thin wall and mag it for cracks. With the cooling design of the 400's you have to have the deck checked too.They warped pretty easily. I know all this stuff from yrs of racing them and from I worked at the Chevy engine plant in Tonn,NY as a engine tester/dyno rm tech.
Now for the safety aspect.I am ticked off by people through their own reckless behavior force the sanctioning bodies to clamp down on safety equipment to the extent where guys are complaining it them trying to create sales for the companies who sell the safety equipment. It costs my racing operation a young fortune to upkeep that now.But I am all about safety and I do see say N.H.R.A.'s point of view that if they didn't take a stand,there would be certain guys that would use Craigslist equipment as so to speak show and tell prevention. But worthless junk.Things like fire suits that where unraveling and 5 point harnesses that where torn.
When it comes to nostalgic racing rules,I know little about that. I would guess they are alittle more relaxed. I do remember the gasser tanks up front and if you remember that,they where mounted to aluminum frames by two spring straps.The idea was in a crash the tank would come out of the mount,but the reality was the tank came out of it's mounting and then smash on the ground or was drag by it not breaking free of the fuel line still attached to it.Not good things happened because of that.
The production Vortec's really only have a 170cc intake runner and that isn't enough for a big inch 400 SBC.It needs in the range of 195 to 200,230 cc's.The Bowtie Vortecs have a raised runner and manifold that is a 200cc intake runner that is going to get you close to what you need.Remember a bigger SBC can accept a bigger cam more easily. You would think so,but Elgin and PBR will grind you a custom cam fairly inexpensively if you call them telling them what you have and where you want to go with it.Make very sure when they tell you to use a valve spring,that is the one you use.
So to sum it up:
400's are useable if handle in the right way
I am a believer in long rod tech
Safety first no matter what rules or what anyone tells you
Bowtie Vortec heads with steam holes drilled
Elgin or PBR cams
Use their recommendations for valve springs.
|I guess what I meant, is there a possible way to set up a old school fuel cell to work properly and safely.... Also f bird 88 on your vortec headed 400 what if u used the same combo but with ik200's? I like this form a lot more then my local one, on cc street scene everyone makes fun of gen 1 stuff there answer to any question is build a ls engine. Gee thanks dickheads lol. I've learned alot just through these past post.
|This thread has more than 15 replies. Click here to review the whole thread.
|Posting Rules
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


text: Mark is a true North Shore native, with an eleven generation lineage, dating back to 17th century Topsfield, Massachusetts. He appreciates what the North Shore has to offer and has always had an affinity for historic homes and New England architecture. Starting at age 20, Mark worked over fifteen years in Research & Development for the most prominent multinational conglomerates, including United Technologies and BAE Systems. His technology background, coupled with a business degree and an enduring passion for real estate culminated when Mark achieved top producer status within months of joining his first real estate firm; he has enjoyed highest sales honors every year since. Mark embraces the latest technologies, which has given him an advantage in promoting and selling homes in today's high-tech marketplace. He has a knack for identifying and accentuating a home's finest features and then formulating the most innovative and effective marketing plan. Since his corporate days, Mark has been intrigued with the art of negotiating and routinely exceeds his client's expectations by delivering to them the best possible price and terms. "I know my clients are getting their best possible result because I treat each deal as if it were my own home sale or purchase." Mark's expertise and professionalism has created a history of satisfied clients, his genuine and good natured character are why so many clients have become his friends. Mark Wade is a member of: The National Association of Realtors® and The North Shore Association of Realtors®


text:
IL-17A increases the expression of proinflammatory chemokines in human pancreatic islets
- First Online:
- Received:
- Accepted:
DOI: 10.1007/s00125-013-3135-2
- Cite this article as:
- Grieco, F.A., Moore, F., Vigneron, F. et al. Diabetologia (2014) 57: 502. doi:10.1007/s00125-013-3135-2
- Citations
- 1.4k Downloads
Cytotoxic T cells and macrophages contribute to beta cell destruction in type 1 diabetes at least in part through the production of cytokines such as IL-1β, IFN-γ and TNF-α. We have recently shown the IL-17 pathway to be activated in circulating T cells and pancreatic islets of type 1 diabetes patients. Here, we studied whether IL-17A upregulates the production of chemokines by human pancreatic islets, thus contributing to the build-up of insulitis.
Human islets (from 18 donors), INS-1E cells and islets from wild-type and Stat1 knockout mice were studied. Dispersed islet cells were left untreated, or were treated with IL-17A alone or together with IL-1β+IFN-γ or TNF-α+IFN-γ. RNA interference was used to knock down signal transducer and activator of transcription 1 (STAT1). Chemokine expression was assessed by quantitative RT-PCR, ELISA and histology. Cell viability was evaluated with nuclear dyes.
IL-17A augmented IL-1β+IFN-γ- and TNF-α+IFN-γ-induced chemokine mRNA and protein expression, and apoptosis in human islets. Beta cells were at least in part the source of chemokine production. Knockdown of STAT1 in human islets prevented cytokine- or IL-17A+cytokine-induced apoptosis and the expression of particular chemokines, e.g. chemokine (C-X-C motif) ligands 9 and 10. Similar observations were made in islets isolated from Stat1 knockout mice.
Our findings indicate that IL-17A exacerbates proinflammatory chemokine expression and secretion by human islets exposed to cytokines. This suggests that IL-17A contributes to the pathogenesis of type 1 diabetes by two mechanisms, namely the exacerbation of beta cell apoptosis and increased local production of chemokines, thus potentially aggravating insulitis.
KeywordsApoptosis Chemokines Cytokines IL-17 Pancreatic beta cells Type 1 diabetes
Chemokine (C-C motif) ligand
Chemokine (C-X-C motif) ligand
Interferon regulatory factor 1
- NF-κB
Nuclear factor-κB
- siCTRL
Negative siRNA control
- siRNA
Short interfering RNA
Signal transducer and activator of transcription
- Th
T helper
Pancreatic beta cells, like neurons and other non-immune cell types, have a vigorous cellular self-defence or cell-autonomous immunity, which contributes to defence against infections (mostly viral) and leads to local inflammation [1, 2]. Key steps of the beta cell-autonomous immune response are regulated by candidate genes for type 1 diabetes, including viral recognition, regulation of inflammation and cross-talk with the adaptive immune response [3, 4, 5]. Excessive pancreatic beta cell-autonomous immunity, in crosstalk with endoplasmic reticulum stress and other signals, exacerbates inflammation, beta cell apoptosis and the progression to type 1 diabetes [6]. The triggering of the full autoimmune response in type 1 diabetes probably depends on a 'dialogue' between beta cells and the immune system, regulated by the local release of chemokines and cytokines, which attract and stimulate cells of the immune system such as macrophages and cytotoxic T lymphocytes [7, 8, 9]. These cells contribute to selective beta cell destruction both directly and via the production of proinflammatory cytokines such as IL-1β, IFN-γ and TNF-α [2].
The production of chemokines by pancreatic islets takes place in response to 'danger signals', such as a viral infection, or following signals provided by dying cells, IL-1β+IFN-γ and TNF-α+IFN-γ [2, 7, 10]. Chemokine expression is also present in islets from type 1 diabetic patients, confirming the clinical relevance of these experimental observations [11].
As insulitis progresses, the nature of local invasion by immune cells probably changes, with an increased contribution by IL-17-secreting CD4+ T cells (T helper [Th] cell 17), together with Th1 cells. Th17 cells have been implicated in several autoimmune diseases, including multiple sclerosis, psoriasis, inflammatory bowel disease and rheumatoid arthritis. Th17 cells produce the cytokine IL-17A, which is also produced by dendritic cells, macrophages and natural killer cells [12, 13, 14]. Neutralisation of IL-17A prevents diabetes in NOD mice when started at 10 weeks of age [15]. Moreover, circulating IL-17+ and beta cell-specific autoreactive CD4+ cells are present in the circulation of type 1 diabetes patients at diagnosis [16, 17] and are associated with diabetes in NOD mice [18]. Importantly, increased expression of IL-17A was detected in the islets of a recently diagnosed type 1 diabetic patient [17] and increased numbers of Th17 cells are present in pancreatic lymph nodes of patients with long-term type 1 diabetes [19]. IL-17A enhances IL-1β+IFN-γ- or TNF-α+IFN-γ-induced apoptosis in rodent and human beta cells [16, 17, 20] and IL-1β+IFN-γ upregulates the expression of the beta cell IL-17A receptor via the transcription factors nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) 1 [17]. This raises the possibility that beta cell apoptosis in type 1 diabetes is initially triggered by Th1 cells and macrophages through the production of IL-1β and IFN-γ, and by other mechanisms, these processes then being followed by the additional pro-apoptotic effects of IL-17A-secreting Th17 cells [17].
As discussed above, the early events leading to the build-up of insulitis involve local production of chemokines, raising the possibility that IL-17A, apart from contributing to beta cell apoptosis, also modulates the production and release of chemokines by islet cells. To test this hypothesis, we characterised the pattern of chemokine expression induced by IL-17A, alone or together with IL-1β, IFN-γ and TNF-α, in human and mouse islets of Langerhans. The data obtained suggest that IL-17A increases the expression and release of key chemokines, in part via activation of STAT1. They also suggest the intriguing possibility that IL-17A contributes to the pathogenesis of type 1 diabetes by two mechanisms, namely exacerbation of beta cell apoptosis and increased local production of chemokines, thus potentially aggravating insulitis.
Ethics statement
Human islet collection and handling were approved by the local Ethics Committee in Pisa, Italy.
Wild-type C57BL/6 mice were purchased from Harlan (Horst, the Netherlands). Stat1 knockout (KO) mice (on a C57BL/6 background) were a kind gift from David Levy (New York University, New York, NY, USA) [21]. All experimental procedures in mice were approved and performed in accordance with the Ethics Committees of the KU, Leuven, Belgium.
Culture of human islet cells, mouse islets, INS-1E cells and the human beta cell line EndoC-βH1
Human islets from 18 non-diabetic donors were isolated in Pisa using collagenase digestion and density gradient purification [22]. The donors (ten women, eight men) were 65 ± 3 years old and had a BMI of 26 ± 1 kg/m2 (mean ± SEM) (electronic supplementary material [ESM] Table 1). Beta cell purity, as evaluated by immunofluorescence for insulin (ESM Table 2), was 56% ± 4% (mean ± SEM). The islets were cultured in M199 culture medium containing 5.5 mmol/l glucose and sent to Brussels within 1 to 5 days after isolation, where they were dispersed and cultured in Ham's F-10 medium containing 6.1 mmol/l glucose (Invitrogen Life Technologies, Paisley, UK) as described [3, 23].
Mouse islets were isolated from 2- to 3-week-old mice and cultured as previously described [21, 24]. After a 24 h recovery period, 150 islets per condition were exposed to cytokines in 1 ml medium.
The rat insulin-producing INS-1E cell line, kindly provided by C. Wollheim (University of Geneva, Switzerland) [25], was maintained in RPMI 1640 GlutaMAX-I medium (Invitrogen) [26].
The human beta cell line EndoC-βH1, kindly provided by R. Scharfmann (University of Paris, Paris, France) [27], was cultured in DMEM containing 5.6 mmol/l glucose, as described [27]. The same medium, but with 2% (vol./vol.) FBS was used for cytokine treatment.
Cell treatment
The following cytokine concentrations were used, based on previous dose–response experiments performed by our group [28, 29, 30, 31, 32]: (1) recombinant human IL-1β (R&D Systems, Abingdon, UK), 10 or 50 units/ml, respectively, for INS-1E cells or human islet cells, mouse islets and EndoC-βH1 cells; (2) recombinant rat IFN-γ (R&D Systems), 100 units/ml for INS-1E cells; (3) murine IFN-γ for mouse islets and human IFN-γ for human islet cells or EndoC-βH1 cells (Peprotech, London, UK), 1,000 units/ml; (4) recombinant murine TNF-α (Innogenetics, Gent, Belgium) for all cell types, 1,000 units/ml; and (5) recombinant murine IL-17A and human IL-17A (R&D Systems) for INS-1E cells and mouse islets, or for human islet cells and EndoC-βH1 cells, 20 ng/ml. Culture supernatant fractions were collected for chemokine measurements.
RNA interference
The short interfering RNAs (siRNA) used in the study are described in ESM Table 3. The optimal concentration of siRNAs used for cell transfection (30 nmol/l) was established previously [23, 33]. Cells were transfected using Lipofectamine RNAiMAX lipid reagent (Invitrogen) as previously described [33, 34]. Allstars negative control siRNA (Qiagen, Venlo, the Netherlands) was used as negative siRNA control (siCTRL). This siCTRL does not affect beta cell gene expression or insulin release, compared with non-transfected cells [33]. After 16 h of transfection, cells were cultured for a 48 h recovery period before exposure to cytokines.
Assessment of cell viability
The percentage of viable, apoptotic and necrotic cells was determined after 15 min incubation with DNA-binding dyes propidium iodide (5 μg/ml) (Sigma-Aldrich, Poole, UK) and Hoechst dye 33342 (5 μg/ml) (Sigma-Aldrich). Determination was by two independent researchers, one of whom was unaware of sample identity [35, 36]. A minimum of 600 cells were counted for each experimental condition. In some cases, apoptosis was also confirmed by caspase-3 cleavage.
mRNA extraction and real-time PCR
Poly(A)+ mRNA was isolated from human islet cells or EndoC-βH1 cells using a kit (Dynabeads mRNA Direct; Invitrogen) and reverse-transcribed as previously described [7, 36]. The real-time PCR amplification reaction was done using iQ SYBR Green Supermix on a LightCycler instrument (Roche-Diagnostic, Indianapolis, IN, USA) and the concentration of the gene of interest was calculated as copies per μl using the standard curve method [36, 37]. Gene expression values in human islets were corrected by the housekeeping gene β-actin, the expression of which is not modified in these cells by cytokine treatment [8, 23], and normalised to the highest value in each individual experiment. The primers used in this study are provided in ESM Table 4.
Total RNA from mouse islets was extracted using a kit (RNAeasy Micro; Qiagen). For cDNA synthesis, 100 ng was reverse-transcribed using Superscript II Reverse Transcriptase and oligo d(T)16 (Invitrogen). Real-time PCR was performed with Fast SYBR Green Master Mix and gene-specific primers or with TaqMan Fast Universal Master Mix and gene-specific primers plus TaqMan probe (Applied Biosystems-Life Technologies, Foster City, CA, USA) (ESM Table 4). PCR amplification was carried out in a real-time PCR system (StepOnePlus; Applied Biosystems). Relative RNA expression was calculated using the comparative cycle threshold method (Ct), after normalisation to the geometrical mean of three housekeeping genes (Rpl27, Hprt and β-actin).
Chemokine ELISA
The human chemokines, chemokine (C-X-C motif) ligand (CXCL) 1 (GRO-α), CXCL8 (IL-8), chemokine (C-C motif) ligand (CCL) 20 (MIP-3α), CCL2 (MCP-1), CXCL10 (IP-10), CXCL9 (MIG) and CCL5 (RANTES) were measured using an ELISA array kit (Custom Multi-Analyte kit; SABiosciences, Frederick, MD, USA) [3]. This is a semi-quantitative assay that does not include a standard curve. Absorbance at 450 nm was measured, corrected by readings at 570 nm and normalised to the geometric mean of β-actin mRNA expression to allow an approximate correction for the amount of islet cells in the preparations. Final results were expressed as arbitrary units. Release of the human chemokines CXCL1, CCL20, CXCL10 and CXCL9 was subsequently measured after STAT1 knockdown (KD) using quantitative ELISA kits (Quantikine; R&D Systems). Release of the mouse chemokines CXCL1 and CCL20 was measured in wild-type and Stat1 KO islets using a quantitative ELISA kit (Quantikine; R&D Systems) and data obtained were normalised to the number of islets.
Chemotaxis assay
C57BL/6 islets were cultured for 24 h as described above and the supernatant fractions collected. For the chemotaxis assay, thioglycolate-elicited peritoneal cells from 8-week-old C57BL/6 mice were seeded in the top chamber of transwell plates (106 cells per well; 5.0 μm) (Costar; Corning, Corning, NY, USA), while conditioned islet medium was added to the lower chambers of the transwell plates. Following an incubation time of 2 h at 37°C in 5% CO2, the cells in the lower chamber were collected and stained for CD45 using a directly conjugated antibody (eBioscience, San Diego, CA, USA). To minimise non-specific binding, the cells were first pre-incubated with anti-CD16/CD32 (clone 2.4G2; eBiosience). Dead cells were excluded by using Fixable Live/Dead Yellow stain (Invitrogen). The absolute number of migrated leucocytes was determined by CountBright absolute counting beads (Invitrogen) according to the manufacturer's specifications. Data acquisition was performed on a Gallios™ flow cytometer (Beckman Coulter, Brea, CA, USA) and Kaluza™ software (Beckman Coulter) was used for data analysis.
Western blot
Cells were washed with cold PBS and lysed using Laemmli buffer. Total protein was extracted and resolved by 8–10% SDS-PAGE, transferred to a nitrocellulose membrane and immunoblotted with the specific antibodies for the protein of interest (ESM Table 2) as described [23]. The intensity values for the proteins were corrected by the values of the housekeeping protein α-tubulin.
Promoter reporter assays
INS-1E cells were co-transfected using Lipofectamine 2000 (Invitrogen) with the internal control pRL-CMV encoding Renilla luciferase (Promega, Madison, WI, USA) and a luciferase promoter–reporter construct containing five NF-κB consensus binding sites (pNF-κB-Luciferase) (BD Biosciences, Palo Alto, CA, USA). Luciferase activities were assayed after 4 h of cytokine treatment.
Whole human islets were collected, fixed in formaldehyde and paraffin-embedded. Sections (5 μm thick) were subjected to antigen retrieval by boiling in 10 mmol/l citrate buffer (pH 6) for 10 min. After overnight incubation at 4°C and with primary antibodies against the chemokines CXCL1, CXCL10 and CCL20, slides were double-stained for 1 h and at room temperature with an anti-insulin or an anti-glucagon antibody. Alexa Fluor fluorescent secondary antibodies (Molecular Probes-Invitrogen, Leiden, the Netherlands) were applied for 1 h at room temperature (ESM Table 2). Immunofluorescence was visualised on a Zeiss microscope equipped with a camera (Zeiss-Vision, Munich, Germany), as previously described [38].
Statistical analysis
Data are expressed as mean ± SEM. A significant difference between experimental conditions was assessed by ANOVA followed by a paired Student's t test with Bonferroni's correction. A value of p < 0.05 was considered statistically significant.
IL-17A exacerbates cytokine-induced apoptosis in rat INS-1E cells, mouse islet cells and in the human beta cell line EndoC-βH1
IL-17A exacerbates cytokine-induced chemokine expression by human and mouse islets
IL-17A induces an early increase in IL-1β + IFN-γ-induced STAT1 phosphorylation and interferon regulatory factor 1 activation in INS-1E cells
It has been previously shown in other cell types that IL-17A activates the transcription factor, NF-κB [39, 40]. NF-κB is important for chemokine expression and cell apoptosis in beta cells exposed to IL-1β+IFN-γ [2, 28]. The addition of IL-17A, however, neither increased IκBα degradation (an early step in NF-κB activation) (ESM Fig. 2a, b), nor augmented IL-1β+IFN-γ-induced activation of a luciferase reporter containing several NF-κB binding sites (ESM Fig. 2c), arguing against a role for NF-κB as a mediator of IL-17A effects in beta cells.
STAT1 KD or KO decreases cytokine-induced apoptosis and the production of specific chemokines in INS-1E cells, and in human and mouse islets
We next examined the effects of STAT1 deletion by using islets isolated from Stat1 KO mice. After 48 or 96 h of IL-1β+IFN-γ or TNF-α+IFN-γ treatment, with or without addition of IL-17A, protection against cytokine-induced apoptosis was complete (Fig. 5c, d), confirming here that, as shown elsewhere [21], STAT1 is a crucial mediator of beta cell apoptosis induced by cytokine mixtures containing IFN-γ.
During the progression of insulitis in type 1 diabetes, pancreatic beta cells are probably exposed to changing groups of cytokines. Recent studies indicate an increased presence of Th17 cells among circulating T cells and in the pancreatic islets of type 1 diabetes patients [16, 17, 18, 19]. Th17 cells secrete the cytokine IL-17A, which we and others have shown to exacerbate IL-1β+IFN-γ- and TNF-α+IFN-γ-induced apoptosis in MIN6 cells and human islets [16, 17]. In the present study, we confirmed that IL-17A augments pro-apoptotic signalling by IL-1β+IFN-γ and TNF-α+IFN-γ in mouse islets, rat INS-1E cells and the human insulin-producing cell line EndoC-βH1, extending our previous observations in human islets and rat beta cells [17]. The use here of a large number of independent human islet preparations, mouse islets and clonal cell lines is particularly important, as it allows solid conclusions regarding the deleterious effects of IL-17A in the context of isolated/clonal beta cells and beta cells studied together with other islet cells. However, a limitation of the present study is its purely in vitro nature, with the isolated islets or beta cells studied being separated from the immune system. It will therefore be important to confirm the present findings in in vivo models or, preferably, in pancreatic or islet samples from type 1 diabetes patients.
Apart from inducing cell death, cytokines also trigger the release of chemokines by pancreatic islets, contributing to the attraction and activation of more immune cells [2, 10, 11]. The chemokine 'signature' of pancreatic islets seems to vary depending on the cytokine stimulus provided. For instance, the pattern of chemokines induced by IL-1β+IFN-γ in pancreatic beta cells is markedly different from that induced by TNF-α+IFN-γ [42]. We showed here that IL-17A alone induces a mild increase in expression of CXCL1, CXCL8, CCL2 and CCL20, in addition to its stimulatory effect on human islets exposed to IL-1β+IFN-γ and TNF-α+IFN-γ. These additive effects are most marked in the case of TNF-α+IFN-γ, affecting, preferentially, the chemokines CXCL1, CXCL8, CXCL10 and CCL20. A similar pattern of chemokine induction was observed in mouse islets. Immunofluorescence studies, including co-localisation with insulin, confirmed that human beta cells are, at least in part, the source of CXCL1, CCL20 and CXCL10 production in human islets.
Chemokines play an important role in the recruitment of leucocytes, monocytes and activated T cells to areas of inflammation in autoimmune diseases [43, 44]. CXCL1 is important in the recruitment of neutrophils, which infiltrate the pancreas and are present in low numbers in the periphery in diabetic patients [45]. CCL2 expression is increased in the islets of prediabetic female NOD mice [7], and transgenic overexpression of CCL2 in beta cells stimulates insulitis and progression to diabetes in mice with a B6D2 background [46]. On the other hand, isolated transgenic expression of CCL2 in NOD mouse beta cells decreases autoimmune-mediated beta cell destruction via induction of tolerogenic dendritic cells, suggesting a context-dependent effect of these proteins [47]. NOD mouse islets also show increased expression of CCL20 and CXCL10, with a peak at around 10 to 12 weeks of life, which precedes the outbreak of diabetes by 2 to 4 weeks. CCL20 is the only ligand for CCR6, which is expressed in Th17 and regulatory T cells [10, 48]. CXCL10 and CXCL9 are present in pancreatic islets from type 1 diabetic patients [11, 48], while infiltrating lymphocytes express the CXCL10 receptor, C-X-C chemokine receptor type 3 [11], suggesting a 'dialogue' between chemokine-producing islet cells and the invading immune cells. In NOD mice, the severity of insulitis is reduced in IL-17- or IL-17/IFN-γ receptor-deficient animals [49], indicating the importance of IL-17A in the attraction of immune cells. As suggested by the present data, this may be mediated via increased production of chemokines.
The mechanisms behind the proinflammatory and pro-apoptotic effects of IL-17A in beta cells remain to be determined. Our present findings rule out a major role for NF-κB and suggest that STAT1 mainly mediates the effects of IFN-γ, but not those of IL-17A. Indeed, KD or KO of STAT1 in human or rodent beta cells prevents apoptosis and the expression of CXCL9 and CXCL10 to the same extent in cells exposed to IL-1β+IFN-γ and TNF-α+IFN-γ, with or without IL-17A. On the other hand, STAT1 seems to have an inhibitory effect on expression levels of the chemokines CXCL1 and CCL20. This may contribute to the generation of specific 'chemokine signatures' depending on the original stimuli and downstream transcription factors activated. It has been shown that IL-17A modulates inflammation in lupus or rheumatoid arthritis via downregulation of the microRNA miR-23b [50], but we were not able to confirm this finding in our model (data not shown).
In conclusion, the present findings suggest that IL-17A contributes to the expression and secretion of chemokines by human and rodent islet cells, but the ultimate mechanisms behind the effects of IL-17A in beta cells remain to be clarified. Our observations indicate that IL-17A contributes to the pathogenesis of type 1 diabetes by two mechanisms, namely the exacerbation of beta cell apoptosis and increased local production of chemokines, thus potentially aggravating insulitis.
We thank M. Igoillo-Esteve, Laboratory of Experimental Medicine-Université Libre de Bruxelles (ULB), for fruitful discussions and help with the analysis of human chemokines by ELISA. We also thank L. Ladriere, ULB, for help with the dispersion of human islets. We are grateful to the personnel from the ULB including I. Millard, A. Musuaya, M. Pangerl and S. Mertens. Thanks also to D. Lambrechts from the Laboratory of Clinical and Experimental Endocrinology (KU Leuven) for excellent technical support.
This work was supported by grants from the Fonds National de la Recherche Scientifique (FNRS) Belgium, the Communauté Française de Belgique-Action de Rechercher Concertéés (ARC), the European Union (projects NAIMIT and BetaBat, in the Framework Program 7 of the European Community) and the KU Leuven (Geconcerteerde Onderzoeksactie 2009/10 and 12/24). F.A. Grieco is a recipient of a postdoctoral fellowship from NAIMIT and was supported by a 1 year postdoctoral fellowship from the University of Siena and from Società Italiana di Diabetologia (Fo.Ri.SID) ONLUS, Italy.
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.
Contribution statement
DLE, FAG, FM, FD, PM and CM contributed to the study concept and design. FAG, FM, FV, IS, OV, LM, DR, HK and LO acquired the data. DLE, LO and CM supervised the study. FAG and DLE drafted the manuscript. All authors revised the article and approved the final version to be published.


text:
Safe mode boot in HP Mini® 210
If you want to boot HP Mini 210 in safe mode, then follow the easy 4-step resolution given below.
By booting in safe mode, you will get an error-free operating system.
Each step is accompanied by an indicative screenshot for the ease of mirroring the action.
Did you know that you can resolve more than 90% of technical problems, quickly and easily? Click on each step in the vertical menu to the left of this page or click on the 'Previous' or 'Next' buttons at the bottom of this section to navigate through the solution.
- Turn on the computer
- Press the 'F8' key
- Choose Safe Mode with networking
- Reboot your computer
Turn on the computer
Turn your computer on so that booting process starts.
Press the 'F8' key
Press the 'F8' key as it is powering on. You want to do this after you see the initial boot screen. If the unit starts beeping at you that means it has already registered that you've pressed 'F8'.
Choose Safe Mode with networking from the menu that appears. This version of safe mode allows you to access the Internet to update any programs you may need.
Reboot your computer when you're done and it will go back into normal mode, or repeat the steps to head back into Safe Mode.
You have successfully booted HP Mini 210 in safe mode with iYogi tech assistance.
iYogi is the fastest growing online and remote tech support provider in the direct-to-consumers and small businesses sector. Our highly skilled and experienced tech experts available, 24x7x365 can provide the best issue resolution and customer satisfaction. Our award winning and low priced Annual Subscription covers the following services:
- Comprehensive tech support to boot your HP Mini 210 in safe mode
- Diagnostic & repair for your technologies
- Troubleshoot software errors
- Update drivers and security to protect against online threats
- Connect to Internet, devices and peripherals
- Optimize your computer's speed and performance


text:
Clarins 'Multi-Active' skin renewal serum 30ml
Item No.1231048145
Item No.1231048145
An exceptional serum which restores a new brightness, youthfulness and vitality to stressed skin marked by early wrinkles, thanks to the action of Nopal flower extract! This unique formula serum restores incredible brightness, softness and a healthy glow. Early wrinkles are reduced and skin appears younger-looking. Better toned, skin is more resistant to aggressions. It is perfectly smooth, incredibly soft to the touch and glows with a new freshness. Apply morning or evening, before your usual day or night moisturiser. Depending on your skin's needs, use as a periodic treatment or all year round.
£160.00 per 100ml
Sunday to Friday - order by 9pm
Saturday - order by 6pm
Monday to Sunday - order by midnight
Orders over £20 - FREE
Orders under £20 - £2
Show full delivery details
Subject to exceptions, Debenhams is happy to exchange or refund your purchase within 28 days of delivery. Please check here for more details on our returns policy and exceptions.
Open a Debenhams Credit Card today and receive:
Please note: if the amount you pay on your card is less than £500 after the 10% discount is applied, the 0% interest offer will not be available.
Credit available from NewDay Ltd to over 18s only
*Terms & Conditions apply.Find out more & apply now


text:
MandM Direct is one of the UK's leading fashion and sports retailers selling your favourite top brands - Bench, Firetrap, Timberland, Fred Perry, adidas, Nike, Puma, Henleys, Berghaus and loads more, all with savings of up to 75% off UK recommended retail prices.
Ratings guide
All Feefo reviews are gathered anonymously from genuine customers. Read more about how our review system works.
A good value summer novelty hat for my toddler
exellent value
Daughter loves it roll on summer
all good
pretty and of good value
Great price in the sale and a very pretty hat, my daughter loves it
Great company to deal with.
Great price & my niece loved it!
everything was super!!!
Excellent quality service and products likewise. I've highly recommended mandm direct to friends and family.
Excellent from start to finish
Lovely hat, loads of comments on how nice it is!
Bargain branded clothing and shoes and super fast delivery ! Highly recommend
I am really pleased with everything I have ever bought from Mandm and I would recommend them to all my family and friends


text: Reason is >>>>> I booked a trip for 4 Adults - 1 child -1- under 2 ( I am paying for it all ) I did it with a Bounce Back __Free Dining - Now I feel like Wow with all the Room Discounts Free D.. ==== etc--- I fell alittleel ripped off ---- Should I call to see I can get anymore off the price I orginally am Paying or just say oh well and enjoy my trip ????


text:
[Date Index]
[Thread Index]
[Author Index]
Re: trig expansion
Murray Eisenberg wrote:
> How can I cause Mathematica to expand Sin[2 Pi omega t + 2 Pi delta],
> where delta and omega are symbols, into the form
> Cos[2 Pi delta] Sin[2 Pi omega t] + Sin[2 Pi delta] Cos[2 Pi omega t]
> without using a replacment rule
> Sin[a_ + b_] -> Sin[a]Cos[b] + Cos[a]Sin[b]
> explicitly?
What is wrong with using a replacment rule? It is probably the best way
to achieve what you want here.
I assume that this question is related to your earlier question (which I
assume is related to Fourier series expansions?):
>I want to make an assignment T = k/omega and somehow cause Mathematica
>to know that k is an integer. How do I do this?
In my opinion, the best way to is using pattern-matching and replacement
rules (see The Mathematica Journal 2(4): 31). E.g., for n integral, we
{Cos[(n_)*Pi] -> (-1)^n, Sin[(n_)*Pi] -> 0};
Paul Abbott Phone: +61-8-9380-2734
Department of Physics Fax: +61-8-9380-1014
The University of Western Australia
Nedlands WA 6907 mailto:paul at physics.uwa.edu.au
AUSTRALIA http://www.pd.uwa.edu.au/~paul
God IS a weakly left-handed dice player


text: Anthony "Toma" Tembelis is a DJ/Producer from New York City. Toma sampled with old hip hop loops and instrumentals originally before getting into the styles of Chicago house and the old New York house since it reminded him of the 80's tracks he grew up on during the 90's. He developed a love for the house seen while living abroad in Tokyo, Japan and got into productions overseas prior to moving back to New York City. Toma's first EP was signed off Chilean label Tempura Records and was put out in early 2013.Anthony Toma's biography Anthony Toma Musician


text:
U.S. Trade and Development Agency:
Limitations Exist in Its Ability to Help Generate U.S. Exports
GGD-94-9: Published: Oct 20, 1993. Publicly Released: Nov 8, 1993.
Additional Materials:
- Full Report:
(202) 512-4800
Pursuant to a congressional request, GAO reviewed the U.S. Trade and Development Agency (TDA), focusing on: (1) whether TDA activities increase U.S. exports; (2) the factors that affect TDA ability to generate U.S. exports; and (3) five other countries' major development projects abroad that increase their exports.
GAO found that: (1) since 1980, over 850 firms have participated in various TDA project planning activities that have generated $3.8 billion in exports; (2) most TDA major development projects have not generated any U.S. exports or have produced fewer exports than originally anticipated; (3) TDA cannot accurately forecast if major development projects have the potential to produce U.S. exports because of the lack of specific export information; (4) TDA believes that many projects will produce more exports in later phases; (5) some government and private officials believe TDA activities are successful even if they do not directly produce exports because they foster relationships within foreign countries; (6) TDA is improving its project management by enhancing data collection and performing program audits more frequently; (7) factors that limit TDA ability to generate exports include its limited and indirect role in the project development process, changes in international political and market conditions, and its dependence on other U.S. agencies for services; (8) the federal government has established the Trade Promotion Coordination Committee to improve export promotion agency coordination; (9) TDA is expanding its project planning activities to include elements of the architectural and engineering design phase; and (10) the five countries reviewed use their foreign aid resources to fund major development projects, have a more integrated approach to identifying and funding projects, and use proactive strategies to foster commercial benefits.
Dec 5, 2016
Telecommunications:Information on Low Power Television, FCC's Spectrum Incentive Auction, and Unlicensed Spectrum UseGAO-17-135: Published: Dec 5, 2016. Publicly Released: Dec 5, 2016.
Oct 5, 2016
Emergency Communications:Improved Procurement of Land Mobile Radios Could Enhance Interoperability and Cut CostsGAO-17-12: Published: Oct 5, 2016. Publicly Released: Oct 5, 2016.
Emergency Communications:Survey of Selected Federal Agencies' Use and Procurement of Land Mobile Radio Equipment (GAO-17-13SP, October 5, 2016), an E-supplement to GAO-17-12GAO-17-13SP: Published: Oct 5, 2016. Publicly Released: Oct 5, 2016.
May 4, 2016
Statutory Copyright Licenses:Stakeholders' Views on a Phaseout of Licenses for Broadcast ProgrammingGAO-16-496: Published: May 4, 2016. Publicly Released: May 4, 2016.
Apr 27, 2016
Tribal Internet Access:Increased Federal Coordination and Performance Measurement NeededGAO-16-504T: Published: Apr 27, 2016. Publicly Released: Apr 27, 2016.
Feb 3, 2016
Telecommunications:Additional Coordination and Performance Measurement Needed for High-Speed Internet Access Programs on Tribal LandsGAO-16-222: Published: Jan 29, 2016. Publicly Released: Feb 3, 2016.
Sep 18, 2015
Internet Management:Structured Evaluation Could Help Assess Proposed Transition of Key Domain Name and Other Technical FunctionsGAO-15-642: Published: Aug 19, 2015. Publicly Released: Sep 18, 2015.
Jun 2, 2015
Broadband:Intended Outcomes and Effectiveness of Efforts to Address Adoption Barriers Are UnclearGAO-15-473: Published: Jun 2, 2015. Publicly Released: Jun 2, 2015.
Telecommunications:FCC Should Evaluate the Efficiency and Effectiveness of the Lifeline ProgramGAO-15-638T: Published: Jun 2, 2015. Publicly Released: Jun 2, 2015.
May 21, 2015
Telecommunications:Agencies Need Better Controls to Achieve Significant Savings on Mobile Devices and ServicesGAO-15-431: Published: May 21, 2015. Publicly Released: May 21, 2015.
Looking for more? Browse all our products here


text:
FILE - In this Oct. 10, 2011 file image released by Harpo, Inc., Oprah Winfrey, right, is shown with host Rosie O'Donnell during the debut of "The Rosie Show," in Chicago. Oprah Winfrey's struggling network, OWN, is laying off 30 workers and restructuring its operations in New York and Los Angeles. On Friday, March 16, OWN announced the curtain on "The Rosie Show" will be on March 30, after five months on the air. (AP Photo/Harpo, Inc., George Burns/file)
Rosie O'Donnell's talk show on the Oprah Winfrey Network is getting the boot.
After just five months on the air, The Rosie Show. hosted by the outspoken star, will tape its last episode on March 20, which will air on March 30.
PHOTOS: Stars who made their return to TV
"I thank Rosie from the bottom of my heart for joining me on this journey. She has been an incredible partner, working to deliver the best possible show every single day," Oprah Winfrey says in a statement. "As I have learned in the last 15 months, a new network launch is always a challenge and ratings grow over time as you continue to gather an audience. I'm grateful to Rosie and the dedicated Rosie Show team for giving it their al.
In its first and evidently only season, The Rosie Show hosted celebrity guests including Russell Brand and Chelsea Handler.
Her interview with Handler deemed to be rather controversial, when O'Donnell confessed to the acerbic E! star, "I'm a little ashamed about it [but] I have a mild fear or anxiety around little people. The problem with me is I can't put the two things together. This is an adult person, a little person...it's so hard for me." Later, she apologized via her Twitter account.
PHOTOS: Oops! Memorable celeb meltdowns
But now that thing have come to a close, O'Donnell is thankful for being included on Winfrey's OWN Network.
"I loved working with Oprah in the amazing city of Chicago," Rosie says. "I was welcomed with open arms and will never forget the kindness of all I encountered. It was a great year for me -- I wish the show was able to attract more viewers -- but it did not. So I am headed back to my home in New York -- with gratitude. On we go!"
Catch the remaining episodes of The Rosie Show on OWN weeknights at 7/6c.
Get more Us! Follow us on Twitter, Friend us on Facebook, Subscribe to Us Weekly


text:
Oregon Historical County Records Guide
Yamhill County Scenic Images
Grain Silo
buy high resolution copy | next image
A grain silo along Kuehn Road. (Photo No. yamDA0018c)
About this image
Dimensions: 3872 x 2571 pixels (300 dpi version); Date created: October 2010; Camera: Nikon D80 Digital SLR; Lens: Nikon AF-S DX Zoom-Nikkor 18-135mm f/3.5-5.6G IF-ED; Photographer citation: Gary Halvorson, Oregon State Archives; Keywords/Tags: horizontal, Oregon, Yamhill County, silo, rustic, grass, blue sky, clouds, rust, rusted, rural, farm, farming, agriculture, farm equipment, hill, Kuehn Road, grain storage.
buy high resolution copy | next image
County guide home | Scenic images home


text:
Simon Cowell Confirms Demi Lovato's "X Factor" Departure
He's always a few steps ahead of the game, so it was no surprise to Simon Cowell when Demi Lovato announced she wouldn't be back for a third season of "The X Factor."
The snarky music mogul confirmed Lovato's exit from his program, telling The Hollywood Reporter, "I always knew she wasn't coming back because she's touring, so I knew that."
Demi added, "I started the show being a singer and a musician, and so I'm going to go back to that. It's been really great, but I'm so excited for 2014. I'm going to dedicate it completely and entirely to music – touring and making a new album. Possibly releasing one."
As for what she'll miss most about the gig, Lovato gushed, "Being able to sit next to Kelly and Paulina and see them twice a week, and also helping mentor the contestants."


text:
Prom Disaster Kit
Your artistic personality shines in classically feminine clothing -- just like Hilary Duff, Gwen Stefani, and Kate Winslet. Getting their look is all about looking soft and pretty regardless of what you're wearing. Pair lots of pretty pieces together and everyone will gravitate toward your infectious personality!
Hilary Duff
September 28, 1987
Scorpio: Celeb Fashion


text:
Botox Q&A
Round Implant + Botox
Narrow 1 question by:
Sort by:
Long Face - Can Botox Make It a Little Bit Round or Square?
My face has always been long but it wasnt that long before. I think its because of gaining and loosing weight thro the years. When i smile it looks... READ MORE
No matching results


text:
Take CableTV.com's Advice and Make DIRECTV Your Next TV Service Provider
Have you been looking for the best TV service provider in Pine Apple? Your search just got a lot easier, thanks to CableTV.com! We take the results of independent consumer surveys and use them to rate the cable companies and television services available in your area. In fact, according to our research, DIRECTV is the top rated provider when comparing both satellite TV and cable companies in Pine Apple, AL.
When you sign up for TV service, most major networks are included with your package, regardless of your location. Unfortunately, that isn't always the case with smaller channels. Take a closer look at plans that appear to be completely identical and you'll find that the smaller channels available with TV packages in Pine Apple won't be the same as they are in another city. That makes channel comparison another important consideration when you're looking for the best cable company in Pine Apple, AL. If you want the most diverse network selection, or enjoy watching every New Orleans Saints or game, a premier package is a great choice. To find out more, use our research to compare the channels and features available from the highest rated providers available in Pine Apple.
Customer service and reliability are key traits of a great TV provider, which is why CableTV.com includes these factors and more when rating providers in your area. If you want to find the best cable company in Pine Apple, AL, our recommendations will help you find the top-rated provider that delivers excellent customer service.


text:
Accommodation Israel Haifa Dan Yishay Resort near PRC Your Review
You need to be logged in to leave a review for Dan Yishay Resort near PRC. If you booked with Travellerspoint, you should have received an email with log in details. If you aren't sure what your details are, please don't hesitate to contact us.
Have you forgotten your username or password? Retrieve Your Login Details
Only post a review if you have actually stayed at this property. DO NOT post a review if you are the property owner or only know about this property through hearsay.
We would also be happy to hear of anything you encountered that could be improved or rectified from Travellerspoint's side, in other words, things that are not directly relevant to your stay at this property but rather to the information on our site or the booking process itself. Was information incomplete or incorrect, did you have problems checking in etc. Please contact us.
Be fair in your review and think of what potential guests might like to know that isn't already explained.
Dan Yishay Resort near PRC is a guesthouse very close to famous Bahaii gardens in Haifa fully equipped for a vacation. It offers three studio apartments with air conditioning, wi-fi, kitchen and private bathroom for each unit.


text:
- Description
Luxurious yet Comfortable, this Upholstered Hand Carved Bed focuses on the European design style. Buttermilk finish with upholstered headboard, this luxurious bed will be a great addition to your bedroom and light up every detail. Not a luxurious furniture, but a great investment in your home. Available in queen size, Eastern king size and California king size.Includes
1 California King Bed, 1 Night Stand, 1 Dresser, 1 Mirror and 1 ChestDimensions
California King Bed, 84"L x 72"W
Night Stand, 32" x 20" x 31"H
Dresser, 68" x 23" x 40"H
Mirror, 28" x 1" x 40"H
Chest, 46" x 20" x 60"H
More from this collection: HD-13002
- Additional Information
Additional Information
SKU 16900554 MPN HD-13002-CK-Set GTIN N/A Brand Homey Design Collection HD-13002 Assemble Length No Assemble Width No Assemble Height No Assembly Required Yes Material Wood Finish No Sizes 5-Piece Sets Bed Styles No Shipping Time 1-2 Weeks Free Shipping No
- Reviews
Disclaimer: Pictures displayed may include optional products not included in the price. Computer monitors often display color variations. Color swatches are shown if alternative colors are available. Please call us or email us if you have any questions!
You may also be interested in the following product(s)
Homey Design HD-13002 Traditional Luxury Dresser$1,190.00
Homey Design HD-8011 Traditional Vienna Euro Button Tufted King Bed$2,371.99
Homey Design HD-7012 Classic Victorian European Button Tuft King Bed$2,541.99
Homey Design HD-7012 Victorian European Button Tuft Cal King Bed$2,541.99
Homey Design HD-20131 Victorian European Classic Estern King Bed$2,590.00
Homey Design HD-20131 Victorian European Classic Cal King Bed$2,590.00
Homey Design HD-20131 Victorian European Classic Nightstand$718.00
Homey Design HD-20131 Victorian European Classic Dresser$1,790.00
Homey Design HD-20131 Victorian European Classic Dresser Mirror Set$2,228.00
Homey Design HD-20131 Victorian European Chest$1,390.00
Homey Design HD-20131 5Pcs Victorian European Estern King Bedroom Set$6,190.00
Homey Design HD-20131 5Pcs Victorian European Cal King Bedroom Set$6,190.00


text:
Member since 2/4/2014
- 1 total post
- 1 question
Questions from Milagrosa
How can I get rid of the big lumps after 12 years of injectable filler? (photos)
Yes, I was intected 12 years ago, it looked ok till 5 years ago, but now that I have age, the lumps are more noticible on the sides of my mouth. To be honest I have no idea or... READ MORE


text:
Depression The angle of depression of an object from an observer viewing the object from above, is the angle between the line joining the object and the observer, and the horizontal plane.
Return from Depression to Math Glossary.
Return from Depression to Math Problem Solving.
Find a Math Word here
Can't find the word you are looking for? Do a search here and type your word in the box below
Good, better best.
Never, ever rest.
Until your good is better
And your better is your best.
Like this website?
Like it on facebook!
"I am getting much better test marks and I have been moved up a set"
Age 13
"High school's great, I'm in a good math set and I understand lots of things, thanks to you"
Eleanor Talbot
Age 11
"I really like math now, you just make it more friendly and normal"
Jess Johnson
Age 18
"Your writing style is great, informative but friendly! My 12 year old son still struggles with basic maths. I can see your website will be a real treasure trove for us :)"
New Zealand
"My grades have improved and I am doing much better than everyone else in my class"
Age 14
"Thanks for teaching me fractions, You made it so easy kalamuni!"
Abrar Haider
Age 8


text:
Coming up in the next {{countdown}} {{countdownlbl}}
Coming up next:
Skip to this video now
Now Playing:
Play Video
This transcript has been automatically generated and may not be 100% accurate.
"Girl Meets World" cast plays charades with Ginger Zee.
Chris Weidman and Frankie Edgar show off their fighting moves with the show's producer.
Now Playing: GMA LIVE! (6.24.14)
Now Playing: 'GMA LIVE!' (6.23.14)
Now Playing: GMA LIVE! (6.20.14)
Now Playing: 'GMA' Hot list: Firework safety tips for the Fourth of July holiday
Now Playing: JK Rowling thanks fans on 'Harry Potter' 20th anniversary
Now Playing: Tom Holland says he had 8 auditions for 'Spider-Man: Homecoming'
Now Playing: Nick Carter teaches 'Spider-Man' Tom Holland classic Backstreet Boys dance moves
Now Playing: Alec Baldwin confirms he will continue playing Donald Trump on 'SNL'
Now Playing: Nick Carter says his 'Boy Band' contestants can 'sing their butts off'
Now Playing: James Blake speaks out on his NYPD ordeal
Now Playing: Best moments of the 2017 NBA Awards
Now Playing: DeMario Jackson speaks out about 'Bachelor in Paradise' controversy
Now Playing: Serena Williams fires back at John McEnroe
Now Playing: Little Big Town performs 'We Went to the Beach' exclusively for the 'GMA' audience
Now Playing: James Blunt performs 'Make Me Better' at 'GMA'
Now Playing: 'Spider-Man' star Michael Keaton confirms role in Tim Burton's 'Dumbo'
Now Playing: Sarah Jessica Parker selects 'No One Is Coming to Save Us' as her first book club pick
Now Playing: James Blunt performs 'OK' live on 'GMA'
Now Playing: Kim Kardashian West reportedly buys Jackie Kennedy Onassis' Cartier watch
Now Playing: 50 Cent and Naturi Naughton dish on the new season of 'Power'
Now Playing: {{itm.title}}


text:
Oregon Historical County Records Guide
Sherman County Scenic Images
Deschutes River
buy high resolution copy | next image
The Deschutes River near its confluence with the Columbia River. (Photo No. sheDA0053)
About this image
Dimensions: 2485 x 3743 pixels (300 dpi version); Date created: June 2010; Camera: Nikon D80 Digital SLR; Lens: Nikon AF-S DX Zoom-Nikkor 18-135mm f/3.5-5.6G IF-ED; Photographer citation: Gary Halvorson, Oregon State Archives; Keywords/Tags: vertical, Oregon, Sherman County, Deschutes River, hills, arid, blue sky, dark water, shadows, dry, tree, branches, silhouette, rural, Wasco County.
buy high resolution copy | next image
County guide home | Scenic images home


text:
Alan Goldhamer DC runs the TrueNorth Health Center in Santa Rosa, California, which specializes in medically-supervised water-only fasting. In this...
News Tagged As:
Entries tagged with: fasting
10 result(s) displayed (1 - 10 of 10):
Water Fasting w/Vegan Diet Cures High Blood Pressure - Dr. Goldhamer (VIDEO)
February 15, 16 at 07:54 PM
BMJ Cancer Case Report: Water Fasting Plus Veg Diet = Cancer Free
December 14, 15 at 10:36 AM
The British Medical Journal recently published a case report involving celebrated experts Alan Goldhamer DC and Michael Klaper MD of...
Resolving Corruption -- How to "Reboot" Your Brain so that Healthy Eating is Preferred
Alan Goldhamer, D.C. and Jennifer Marano, D.C. | April 17, 13 at 09:56 AM
Adopting a health-promoting diet in the modern world is among the most difficult challenges facing humans living in industrialized...
A Fast Way to Health -- Water-only Fasting
Alan Goldhamer, D.C., Director TrueNorth Health Center | April 24, 12 at 10:45 AM
Fasting to achieve physical and psychological health benefits has been practiced for thousands of years. However, until recently, scientists had...
FASTING: Safe & Effective Use of an Ancient Powerful Healing (VIDEO)
December 7, 11 at 06:35 PM
If you suffer from any of these conditions or other chronic health problems, you could greatly benefit from learning about...
Famed Vegan MD Michael Klaper Moves Practice to California
December 21, 09 at 10:23 PM
Michael Klaper, MD, Joins Staff at TrueNorth The following is an interview with Dr. Klaper Dr. Klaper, you've had...
On-off fasting helps obese adults shed pounds
YAHOO.COM | Anne Harding | November 13, 09 at 12:23 PM
Fasting every other day can help obese people lose weight, a small study hints. Even though the study participants ate...
Fasting to save my life - should I go veg or vegan?
November 13, 09 at 06:16 AM
Fasting -- Back To The Future: The Rediscovery of Water-Only Fasting
Alan Goldhamer, D.C. | November 6, 04 at 03:54 AM
Although the notion of electing to go without food for prolonged periods of time to improve one's health has not...
My Fasting Experience
Mark Epstein | November 6, 04 at 01:39 AM
Last winter, I began to think about taking some time off to personally experience a medically supervised water-only fast. I...


text:
APOLLO-RIDGE ELEMENTARY: Prinipal welcomed
SPRING CHURCH — Apollo-Ridge elementary has a new principal this year. Her name is Courtney Anderson.
Last year, Anderson was the assistant principal in our school. She feels she learned a lot last year. Anderson says being a principal takes a lot of hard work.
She handles disciplining students, helping teachers and parents and running the school. Principals also work over the summer while the students are enjoying their vacation. During this time she worked on scheduling new things for the school. When Anderson is not at work, she enjoys watching her children play soccer. She also plays the game Candy Crush and enjoys reading and the website Pinterest.
Anderson is a great principal who works hard to make sure all of her students get a good education.


text:
Targeted at individual accountability and focused around training, discipline and standards, Know The Signs encourages leaders and soldiers to first Know the Signs, because someone always knows when a soldier is at risk. Then, Know What's Right (know what right looks like; know the standards, regulations, guidelines and laws), and then, Do What's Right (break the cycle of an accident waiting to happen by taking action and intervene before the loss or injury). With a majority of off-duty Army accidents currently attributed to indiscipline, this campaign also reminds soldiers that standards apply 24/7, whether on duty or on the road for a ride with friends.
Video provided by: IMCOM (Army Installation Management Command)
Disclaimer: The appearance of hyperlinks does not constitute endorsement by the Department of Defense of this website or the information, products or services contained therein. For other than authorized activities such as military exchanges and Morale, Welfare and Recreation sites, the Department of Defense does not exercise any editorial control over the information you may find at these locations. Such links are provided consistent with the stated purpose of this DoD website.


text:
Loki - Interview @ GameSpot
Cyanide's Thomas Veauclin has been interviewed at GameSpot about Loki in a conversation that again stresses the hack'n'slash over story or other elements, which I guess is refreshingly realistic:
Yes, it's always refreshing when people tell it like it is in the game biz--after reading a few PR releases, it often feels like they are all the same game, just being pushed by different snake-oil salesmen.
And it's about time they took the 'rpg' out of action-rpg. ;)
Most of all the game is full of bugs and totally unfinished. I am playing the german version now and it's quite clear that the game has been released 6 months or more too early. This pattern seems to apply to far too many games released nowadays.
|All times are GMT +2. The time now is 07:44.
Powered by vBulletin® Version 3.8.10
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Copyright by RPGWatch


text:
21 Apr
Technical Officer Scientific Asst And Artisan - - Electronics Corporation India Limited Ecil
- Position
- Technical Officer Scientific Asst And Artisan
- Company
- Electronics Corporation India Limited Ecil
- Location
- Opening
- 21 Apr, 2017 30+ days ago
Electronics Corporation India Limited Ecil as the company that open the jobs vacancy, have some qualification and spesification especially for the Technical Officer Scientific Asst And Artisan jobs vacancy. To find out more information and about qualification and spesification details, walkin interview schedule, the address of the company, the company contact info (email/phone number) of Electronics Corporation India Limited Ecil company, please start to apply for the job vacancy with fill the jobs application with click the 'Apply This Job' button below.
Recruitment 2017 - ECIL, a Public Sector Enterprise under Department of Atomic Energy is looking for dynamic and
result-oriented persons for the following positions on contract basis initially for a period of one
year (extendable by one more year depending upon on project requirements) having
maintenance experience to work at ACS/NPCIL-Kaiga, ACS/NPCIL-Narora, C&I/NPCIL-Kota &
Tarapur and ACS/NPCIL-Kakrapara as per the Site requirements for CSD
Sr. No.
Name of Post
No. of Vacancy
Age Limit
Technical Officer on Contract
30 yrs
Rs. 21,000/-
Scientific Asst-A on Contract
25 yrs
Rs. 13,356/-
Junior Artisan on Contract
25 yrs
Rs. 11,764/-
Sr. No.
Qualification & Exp.
A First class Engineering Degree in Electronics and Communication / Electrical & Electronics / Electronics & Instrumentation with minimum 60% marks in aggregate from any recognized Institution / University with post qualification experience in the field of RF Communication / IT-H/W,S/W / Networking / Testing / Maintenance of Control & Instrumentation/Electronics & Communication.
A first class Diploma in Engineering with at least 60% aggregate marks from any institution recognized by the State Board of Technical Education in Electronics and Communication / Electrical & Electronics / Electrical / Electronics & Instrumentation. Should have post qualification experience in the field of Testing / Maintenance of Control & Instruments / Electronics & Communication systems.
Should have passed ITI (2 years duration) in the trades of Electronics / Electrical / Computers / Instrumentation / Fitter. Should have post qualification Industrial experience in the field of testing / maintenance of Pneumatic & Electronics Instruments / Communications gadgets / Electronics Systems / Computers. How to Apply : Eligible candidates may download the application format from our website www.ecil.co.in and attend the written test / trade test for Scientific Asst-A on contract and Jr. Artisan on contract from 0900 hrs. to 1100 hrs., on 06.05.2017 as indicated at Interview Venue and Placement Details below. The Interview for Technical Officer on contract will be conducted between 0900hrs., to 1100 hrs., on 06.05.2017 as indicated at Interview Venue details and the eligible candidates should attend with duly filled in application along with all original certificates in support of date of birth, qualification, experience and caste etc., with one set of photocopies and recent passport size colour photograph.
The selection will be based on interview performance (for Technical Officer on contract) and written / trade / practical test (for Scientific Asst-A on contract and Jr. Artisan on contract). ECIL reserves the right to cancel/restrict/enlarge/modify the vacancies notified/recruitment process if need so arises, without assigning any reason.
Interview and Placement Details :
Delhi - ECIL ZONAL OFFICE, B-7, DDA Local Shopping Complex, A-Block Ring Road, Naraina, New Delhi - 110 028, Ph.No.011- 25774645/011-25777676
Mumbai -ECIL Zonal Office, 1207, Veer Savarkar Marg, Dadar (prabhadevi), Mumbai-500 028. Ph.Nos.022-24302664/ 24300034
Bengaluru - ECIL Branch Office, No.1/1, 2nd Floor, Jeeven Sampige, Lic Building, Sampige Road, Bengaluru - 560003. Ph.no.080-23460110


text:
Your family loves variety and you love deals. Buy one of our inexpensive Golden Corral gift cards and treat yourself to a salad bar, pizza and tons of other great favorites. Use the card in any of the participating Golden Corral locations and have a family night out""you'll love how much you save on dinner!
CardCash is the largest gift
card exchange in the world.
Turn unused gift cards into cash
or buy discount gift cards with the
safety and security of our 45 day
card balance guarantee.