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Sabudana chikodi as we call it in marathi is prepared with tapioca (sago) & used as one of the fasting snacks . This can be either prepared plain or with food colours added. Also tomato or mint juice can be added to get different flavors.My mom used to make this during summer vacation.
This takes me down the memory lane of my childhood days when I stayed in uran .Summer is the season when women (especially in maharashtra)prepare their yearly stock of pickles,moramba (mango jam),mango chunda & walwan ie dried items like papad ,sabudana chikodi, potato wafers ,sabudana aloo chakali (murukku) which can be used for fasting too ,kurdaya ,sandge, mirgunda, walaveli tondli etc.
I remember my aaji (moms mausi) used to fill big glass jars with pickles & handover jars to all her daughters .
She would even prepare ambepoli ( wadi prepared from alphonso mango pulp), sun dried kokam sherbat,amboshi ,papads ,kurdais.My mom prepares variety of pickles all round the year.
As a child I too was a part of this program especially batata papad ,sabudana chakali ,sabudana chikodi & potato wafers (all can be eaten during fast (vrat/upwaas) which my mausi used to prepare.
My work was to dry these items on a cot ( a bed made of wood & coconut coir),turn the papads & keep a watch on sparrows,crows & silky (my favourite pamerean dog) that tried to feast these delicious dishes.In fact in uran the women used to schedule papad & collectively gather at each others place & make different kinds of papad. We get tasty urad ,poha,batata & sabudana papad in PEN a small town near alibag which is famous for ganesh murtis.
Updated post with sabudana chikodi made by me in april 2013 in bangalore in which i have used red ,orange & green food colour
I love all four dishes but today want to post about sabudana wafers /chikodya/chikwadya.you can even call them sabudana papad
Here I have added cumin seeds to get a wonderful taste and red,green & orange food colour to make tricolour chikodis .
Sago/sabudana /tapioca- 1 cup
Water - 2-3 cups
Green chilies 3-6
Cumin seeds 2-4 spoons
Food color (optional)
1.Wash Sago & Soak in water for 5 minutes .
2.Then grind the chilies to a coarse paste
3.In a sauce pan, boil water. Add sago to it. Continue cooking over low-medium heat...with constant stirring.dont forget to stir. You will notice it turning to translucent to glassy appearance, much like a glue.. Then remove from heat.
4. Now add salt,cumin seeds & green chilli paste . Mix well now add 1/4 of this mixture in 2 separate bowl & add pinch of green colour & orange colour respectively to sago mixture & mix well.
5.Take a clean plastic sheet or one could use cotton churni/cloth also. Place this sheet under direct sun rays.place some weight on the plastic sheet so that it will not get blown by wind.
6.Now carefully take a spoon full of sago porridge and uniformly place on the sheets.Spread it in the form of circle with the help of spoon. Leave enough gap between 2 chikodis. Now sun dry them for couple of days to a week or until they are dry completely without any moisture.
7.Remove these from the sheets and store in an air-tight container.
8.heat oil in a pan. When the oil is hot enough...fry few fryums at a time... it takes less than 20 seconds to get fried...so remove immediately using slotted spoon.
9.Serve as snacks or on upwas /fasting day or as side dish with dalfry rice/sambhar rice /rasam rice.


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Celebrate your special day in the charming, convenient location of one of Salem's historical gems. Mission Mill Museum provides a beautiful romantic setting for your special day. The millrace rushes musically by, and delightfully restore historic buildings provide the perfect photo backdrop. Whether your event requires space for party of 50 or over 400, our lush five acres site can accommodate you. We offer both indoor and outdoor options. Parking on-site.
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At first, it seems surprising that a vaccination is central to recent presidential "debates" until one realizes how potently this important public health issue illustrates the erosion of public trust in authority.
Rick Perry took significant campaign donations from Merck & Co, the manufacturer of the HPV vaccines, and then signed an executive order to mandate the HPV vaccine in Texas. In an attempt to discredit Perry, Michelle Bachmann claimed that a girl was made retarded by the HPV vaccine. She has been ridiculed for making this statement, and while it may be the wrong argument, it is correct information.
In February 2007, Governor Rick Perry bowed to the lobbying pressure of Merck and attempted to mandate Gardasil for all middle-school-aged girls in Texas. Merck donated $16,000 to Perry's campaign in the two and a half years prior to his signing the executive order to do so. Michael Toomey, Perry's former chief of staff, was one of Merck's top lobbyist at the time Perry signed the executive order. The HPV vaccine costs between $360 and $390 for the three shot series and would have cost the state of Texas $50 million in the first year alone.
At the time, Merck hoped to generate as much as $3 billion in annual sales from Gardasil. According to Forbes, the vaccine is a disappointment to Wall Street analysts, who expected it to generate from $2 billion to $4 billion in yearly sales. It generates $1 billion annually in sales.
Medical evidence points to the need for caution in regard to Gardasil. An article in the February 28 2007 issue of the Journal of the American Medical Association (JAMA) found that while HPV infection is common among US females 20 to 24 (44.8 percent are infected), only 24.5 percent of girls 14 to 19 are infected.
In April 2010, the Indian Council of Medical Research suspended the cervical cancer control vaccination program for girls in India after four deaths and more than 120 complications were reported by young women who had received the Gardasil vaccine.
Cervical cancer is the fifth cause of death among women in developed countries with a rate of three cases per 100,000 women, and is still epidemic in developing countries. Seventy percent of HPV infections resolve within one year; 90 percent resolve within two years. Only 10 percent of infections will persist and 50 percent of these will be cancer precursors.
Mass screening programs for HPV infection have had dramatic effects. When 70 percent of women in a society participate in mass screening the rate of cervical cancer drops. Finland saw a 75 percent drop in cervical cancer when women participated in mass screening. Of those who get cervical cancer, 50 percent never got a pap smear.
According to Diane Harper, MD, MPH, MS., the leading international expert on HPV vaccines and developer of Gardasil and Cervarix, the vaccines are highly effective against most types of HPV viruses, but not all of them. Coverage requires three doses of the vaccine and is expensive. No efficacy trials in girls younger than fifteen years of age have been done and the duration of efficacy is unknown for all recipients.
Screening is still essential and screening alone is as effective as screening with vaccines in preventing HPV infection, according to Harper. On the package inserts, Gardisal publishes efficacy of five years and Cervarix publishes 7.5 years. "If HPV vaccines are not effective for at least 15 years, then no cervical cancer is prevented, only postponed," says Harper.
In general, the vaccine has proven safe for most women, but results from the Vaccine Adverse Events Reporting System (VAERS) indicate that 29 percent of vaccine recipients feel dizzy and faint. More than 18,000 reports of adverse events associated with the HPV vaccine have been made since 2006 including 72 cases of death. The risk of adverse events associated with the HPV vaccine is 7 events per 100,000 vaccinated, while the risk of cervical cancer in the developed world is 3 cases per 100,000 women.
CBS covered the story of Gabby Swank, who became very ill after receiving the HPV vaccine. This is probably the girl Bachmann was referring to as she became increasingly mentally confused before she died. Other families of children damaged from Gardasil also tell their stories. Art Caplan's claim that there are no side effects to the HPV vaccine is arrogant in its ignorance and cruel in its dismissal of the real suffering of many families. I hope he will give the $10,000 to Gabby's mom, Shannon.
A 2009 study in Pediatrics found that a majority of parents are concerned about adverse events of vaccine. 99 percent of respondents to an online survey agreed with the statement, "Getting vaccines is a good way to protect my children from disease." However, 54 percent agreed with "I am concerned about serious adverse events of vaccines." 11.5 percent had refused at least one vaccine recommended by their doctor.
Congress has acknowledged that vaccines can have adverse events. Occasionally, a vaccine is even recalled. In 1986, Congress passed the National Childhood Vaccine Injury Act (NCVIA) to protect the vaccine supply by reducing the liability of the vaccine manufacturers and to respond to public health concerns. The NCVIA requires all health care providers to report certain adverse events following vaccination to the Vaccine Adverse Events Reporting System (VAERS). According to the CDC, in 2008, more than 25,000 reports of adverse events in the US were received by VAERS. The NCVIA also created the National Vaccine Injury Compensation Program (NVICP) to compensate those injured by vaccines on a "no fault" basis.
When medical experts such as Mr. Caplan categorically deny the existence of something that parents know exists, experience themselves and are concerned about, it just further erodes parent confidence in the vaccine program. If you would like to research adverse events of HPV yourself, you can go directly to VAERS, or ask Stephen Rubin, PhD a question on our discussion forums. And, many other Gardasil and HPV resources are available on Mothering.
Tags: CDC, Cervarix, Gardasil, HPV Vaccine, Merck, Michele Bachmann, NVICA, Rick Perry, VAERS
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The City today welcomed the lifting of the ban on BP bidding for new US federal government contracts.
After months of discussions, the FTSE 100 oil giant came to an agreement with the Environmental Protection Agency last night that will enable it to sign US government contracts following BP's involvement in the 2010 spill at the Deepwater Horizon oil rig in the Gulf of Mexico.
BP will be able to pitch for new government business as long as it has an independent auditor, approved by the EPA, who will conduct an annual review of its practices.
"This is undoubtedly good news and a relief," said Liberum Capital analyst Andrew Whittock.
"It's a big deal that represents a step back to normal for BP in North America," he added.
BP's shares jumped by 2.45p, or 0.9%, to 482.4p.Reuse content


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Food for All? Marin Conversations with Whole Foods' Walter Robb and Sierra Orchards' Craig McNamara
Broader access to fresh, healthy food and the role of the agricultural community in food supply chains have become important policy issues in the U.S. today. California's agricultural businesses and progressive companies like Whole Foods Market are working hard to find solutions to these concerns as well as dealing with other important issues at the intersection of food, agriculture and society. Please join Whole Foods Co-CEO Walter Robb and Sierra Orchards Founder Craig McNamara for Marin Conversations' discussion of these critical issues.
Please join our monthly conversation between Marin personalities and subject matter experts on important social issues, presented by The Commonwealth Club and Marin Community Foundation. Marin Conversations take place on the first Wednesday of the month (except May 14, 2014) at the historic Outdoor Art Club, One West Blithedale Avenue (corner of Throckmorton) in downtown Mill Valley.
Light dinner fare and cash bar is available until 7:45 p.m. The program runs from 7:45–9 p.m. Attendees are also welcome to a 10 percent discount at Piazza D'Angelo on the evening of the program. Please email any questions to email@example.com.


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Woman Injured in Accident with Fuel Truck
One woman from Spotyslvania County was killed in an accident that happened on Wednesday.
Details about the Fatal Accident
The Richmond Times-Dispatch is reporting that a woman from Spotsylvania was killed in an accident that happened on Wednesday after her vehicle hit a fuel truck along Courthouse Road near Brokenburg, according to state police.
The woman has been identified as 45-year-old Christine Renee Gibbs, who was ejected from her vehicle and was killed at the scene of the accident at about 1:25 p.m. She was not wearing a seatbelt at the time of the accident.
The woman was reportedly heading west when she ran off the right side of the road, overcorrected, and hit a freightliner fuel truck that was heading in the opposite direction. The driver of the fuel truck was taken to the Mary Washington Hospital, but his injuries did not appear to be life-threatening.
At this time, it is unknown what caused the woman to drive off the road prior to the accident.
Important Information about Motor Vehicle Accidents
According to data from the National Highway Traffic Safety Administration, there are thousands of wrongful deaths of people involved in motor vehicle accidents every year in the United States:
- In 2014, there were a total of 32,675 people who were killed in motor vehicle accidents in the United States.
- There were about 219 fewer fatalities in 2014 than there were in 2013.
- There were about 25,000 more people injured in motor vehicle accidents in 2014 than there were in 2013.
$1.8 MillionExpenses: $20,000.00 | Attorneys Fees: $765,000.00 | Net to Client: $1 Million
$2 MillionExpenses: $78,475.96 | Attorneys Fees: $850,087.96 | Net to Client: $1,071,436.00
$2.3 MillionExpenses: $200,000.00 | Attorneys Fees: $900,000.00 | Net to Client: $1.2 Million


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Upgrading from 2006 multiuser edition. Using Peer-to-Peer network. Data files were installed on a remote NAS drive plugged into router.
It looks like I can't use this configuration with 2008. Why? Dedicating one peer machine as the "Server" with data files is not optimal.
Hi ct, starting with the Peachtree Accounting 2007 release, the Pervasive database engine requires certain files to run/execute on the server. In prior releases of Peachtree by Sage, multiple instances of the gateway engine ran on each workstation. Now, only one instance runs on the server and manages all of the traffic transfered over the network which greatly enhances reliability of the program.
Unfortunately, you will not be able to run the Pervasive database executable files on a Network Attached Storage (NAS) device or computer using an operating system other than Windows Server 2000 and higher.
Will Windows XP Home/Pro work in place of MS server 2000? We too are using Multi 2000 version of Peachtree and looking to update too the newest version. We do use a peer system but use a peer server for Peachtree, data storage, and system backups. This peer server currently runs Win XP pro. Would the latest and greatest PT Multi user work on this operating system.
Thanks. Glad to see a Peachtree run forum.
Good, a senior support specialist.... so maybe you can answer some questions about runing the server process on Linux....
Why wouldn't it be a simple matter of running pervasive 10 on Linux and moving the database to the Linux server?
Why don't the accounting companies (sage and intuit) understand that their clients are looking for more sophisticated critical storage solutions, including Linux and NAS servers?
Zigzag,That's a fair question, and having those and additional storage options offers different benefits to different users. Like anything, we focus our attention where there is greatest need among current and potential Peachtree users. The use of Linux and NAS servers among small business Peachtree users is still quite limited. I would imagine the same is true for users of competing products.We'd like to hear from others on this topic. What other server and data storage solutions are being used for which you'd like to see multi-user versions of Peachtree developed?
Mike Savory
Peachtree Product Manager
Move to Microsoft SQL database ( ACT sits on that one already). I just upgraded to Quantum and frankly, the speed increase when interrogating the system ( especially in large inventory files with 4-5 or more fields) is relatively insignificant. A typical inventory report takes 25 second to load.
I am running a fully loaded Dell 1900 in VMWare with 4 Clients enabled.
cell 650-888-1622
PS I upgraded last week. Went pretty smooth but had to pull in the SR2 release on several PC's before the error disappeared.
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Sandy » Change has become a constant at Alta View Hospital, and today patients can check out some of those changes without an appointment.
The hospital is hosting an open house to unveil the Alta View Specialty Center, a new outpatient operating and clinic facility. The 60,000 square-foot center will double the hospital's capacity for many procedures and free up desperately needed space at the hospital.
"It's one of the few times you get to go into the OR standing up," joked Susan Tanner, the center's director of operations.
At the open house, which runs from 4 to 7 p.m. today, prospective patients can view the four new endoscopy rooms, each a roomy 650 square feet. The extra space was almost overwhelming at first, said Dixie VanWinkle, the nursing supervisor. Space at the old facility is so tight, medical staff have an elaborately choreographed dance to move about the room. Now monitors and computer screens operate on booms, freeing up floor space and making it easy to move about.
The facility can handle almost any type of outpatient surgery, said Pua Jorgensen, director of surgical services. Even minor general surgery cases can be accommodated. The facility is currently limited by equipment, but has plenty of room to add general surgery apparatus if the need arises.
In addition to the new endoscopy rooms, the facility features four out-patient surgical suites with plenty of preparation and recovery rooms. An entire wing of the building is devoted to new clinic space with extra exam rooms, including specialty rooms for audiology and ophthalmology.
The space is expected to fill quickly, Tanner said, and while the new bays mean more OR time for physicians, patient need is constantly increasing. The neighborhood around the hospital is seeing a demographic shift to older adults, rather than young families, meaning an increased need for medical care. The hospital recently opened a senior care clinic, specializing in health issues for those older than 55.
Patients and medical staff will begin using the new facilities on Monday. The specialty center frees up space at the Sandy Clinic next door, which undergoes a remodel starting July 1.
Elbow room at Alta View
The public is invited to attend the center's open house tonight, 4-7 p.m., at 9450 S. 1300 East, Sandy. The tour includes the new surgical center and the clinic space. For more information, visit www.intermountainhealthcare.org/hospitals/altaview.


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Applying Hybrid Multi-Cloud Architecture to Design Commerce Ecosystem Community Blog
Authors: Balakrishnan Sreenivasan, Chief Architect – CBS Client Services, Ram Ravishankar, DE, CTO – Hybrid Cloud. Contributing Editor: Mahlon K. Coplon, DE, CTO Digital Commerce Abstract Many retail organizations are thriving to drive...
from Blog: Hybrid Cloud ArticlesModified on by basreeni
IBM proves weather is business Community Blog
MichaelMarinier Tags: bain cloud trial sector bluemix company utilities blackstone africa free retail transportation platform-as-a-servive public @dvworks_africa group trave energy nbc facebook paas weather insurance government developerworks capital universal agriculture 3,469 Views
On March 31, 2015 IBM announced a partnership with The Weather Company . The Weather Company includes the Weather Channel, weather.com, Weather Underground, Intellicast.com and third-party publishing partners. The company provides millions of people every day...
from Blog: developerWorks Africa
IBM proves weather is business
MichaelMarinier Tags: sector developerworks paas africa facebook nbc platform-as-a-servive bluemix weather transportation blackstone cloud public retail @dvworks_africa universal company trave group energy government bain utilities capital trial agriculture free insurance 4,364 Views
On March 31, 2015 IBM announced a partnership with The Weather Company . The Weather Company includes the Weather Channel, weather.com, Weather Underground, Intellicast.com and third-party publishing partners. The company provides millions of people every day...
from Blog: developerWorks AfricaModified on by MichaelMarinier
Experiments with NoSQL for WebSphere Commerce - Part 2 Community Blog
Anbu Ponniah Tags: promotions bluemix ecommerce retail architecture mongodb solution personalization cloudant websphere_commerce nosql 6,235 Views
In part 1 of this blog we had seen how data that is handled in an ecommerce system could be bucketed into various perspectives and categorized into the role it plays or supports. And we discussed two use cases which I shall restate here: ...
from Blog: Commerce Lounge
IBM Academic Initiative Midwest November 2014 Update Topic Data Management: IBM Holiday Shopping Tracking through IBM ExperienceOne, Data Warehouses and Information Integration Teaching Topic, Information Governance Teaching Topic, IBM X-Force Threat Inte Community Blog
firstname.lastname@example.com Tags: university holiday retail analytics marketing academic 1,903 Views
Academic Leaders, first let me say Happy Thanksgiving and thank you for all the work you do to create a thriving learning environment. With the holiday season approaching this is a great time to introduce the new IBM ExperienceOne tool that was...
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Optimized Solution for Oracle Retail on IBM Power Systems Community Blog
Pat Moore Tags: retail_datasheet powersystems_datasheet powersystems prs5274 datasheet retail_powersystems_datas... retail retail_powersystems 2,263 Views
from Blog: IBM and Oracle
Why will business buy a lot more analytic software? Because of customer attributes!
daryl_pereira Tags: ibminsight retail analytics 2,320 Views
Analytics software will grow 3-4 times faster than traditional IT software. Forrester calls it "Business Technology". Why? Because its function is directly tied to specific customer use cases such as Customer Acquisition, Customer Growth (e.g....
from Blog: IBM Developer Ecosystem BlogModified on by daryl_pereira
Wie frisch ist online? Oder: Cross Channel Funktionalität im Lebensmittelhandel Community Blog
Burkhard Schulte Tags: ordermanagement smartercommerce retail 801 Views
Mit zusätzlichen Services rund um den digitalen Einkauf erweitern elektronische und stationäre Händler zurzeit ihr Angebot. Für viele Handelsbereiche ist es heute mittlerweile der Mindest-Standard, Waren online zu kaufen, lokal abzuholen oder in einer...
from Blog: Blue Blog
How to build customer intimacy and an omnichannel experience: the IBM Ecosystem explains Community Blog
daryl_pereira Tags: retail sapientnitro findability nrf14 aginity ibm 869 Views
The retail space is going through incredible transformation. The channels customers are using to buy and interact with brands are proliferating faster than you can pin an Etsy brooch on Pinterest. At the same time, the technology and data available to...
from Blog: IBM Redbooks Software Blog
IBM Watson all the way Community Blog
Shweta Gupta IBM Tags: ibm conference watson ginni retail 2,869 Views
Ginni Rometty is infectious in her excitement. The announcement of the new IBM Watson Group headquartered in the New York City is for each of us @ IBM big technology shift, and for the world, a changer. http://abcnews.go.com/Technology/wireStory/ibms-watson-su...
from Blog: WebSphere Commerce peeled or searched !!


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Debenhams Beauty Box Mini Lip Glosses Gift Set
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I have been writing something or other since I was a teenager. Some poems have been published in poetry collections [local contests].
It has been said that writing can be a lonely occupation.
There are local writer's groups who meet for critiquing and encouragement, as well as plenty of communities online which give one the option of posting stories or poems.


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Grit Guard
> Grit Guard« Dual Bucket Washing System - BLACK
Grit Guard« Dual Bucket Washing System - BLACK
No more wash-induced swirls!
The Grit Guard Dual Bucket Washing System makes washing your car safer and simpler. With a wash and rinse bucket, you can keep your wash water free of dirt and paint-scratching particles. The Double Dolly ties it all together. Your Grit Guard Dual Bucket Washing System rolls wherever you need it to make washing easier and more beneficial for your vehicle!
The principle behind the two bucket wash method is simple: by rinsing out the wash mitt before reloading it with soap, much less dirt is returned to the vehicle. Therefore, scratching is less likely. Use the two bucket method once and you'll be hooked. Just look in the rinse bucket when you're done washing for proof positive that the two-bucket method works!
The Grit Guard Dual Bucket Washing System takes the idea of the two bucket method and makes it a reality - on wheels! Now there's no need to run back and forth from the wash bucket, losing valuable suds along the way. The Grit Guard Dual Bucket Washing System rolls around with you on multi-directional casters. Your wash and rinse buckets are in easy reach while you work around large vehicles, RVs, and boats.
The Grit Guard Dual Bucket Washing System includes
2 - 5 Gallon Wash Buckets
Use one bucket for soapy water and one for rinsing. This two bucket method helps keep dirt from being transferred back to the vehicle. Each bucket has its own Grit Guard Insert and Gamma Seal« Lid.
Grit Guard Inserts
The Grit Guard is a raised grid surface that sits in the bottom of the included 5 gallon buckets. Fill the bucket with your favorite shampoo and water and place the Grit Guard in the bottom. Load your mitt with soapy water and wash as usual. When you dunk the mitt back in the bucket, drag it across the grid to remove dirt that has accumulated in the nap. The dirt will fall between the slats and settle in the bottom of the bucket. Even though you agitate the top water every time you reload the mitt with soap, the Grit Guard stabilizes the water in the bottom of the bucket so the dirt does not rise.
Gamma Seal« Lids
These lids form a watertight seal. You can fill the buckets with water and transport them without the lid leaking! For car collectors who participate in car shows, this is a great feature. Believe it or not, some of these enthusiasts use nothing but distilled water on their vehicles and theyÆll actually bring it to the car show if itÆs not available on site. ThatÆs dedication! The lids also keep dust out of the buckets when they're not in use.
2 Grit Guard Dollies
No wash bucket is complete without the 5 Wheel Car Wash Dolly! The dolly accommodates any 3.5 - 7 gallon round bucket and has 5 heavy-duty casters capable of supporting up to 250 pounds. The dolly makes your wash bucket mobile so you can easily roll it around a vehicle as you wash it.
Two locking casters hold the dolly still on an incline. Three thumb screws hold the bucket in place inside the dolly. The durable, metal casters turn in any direction to give the wash bucket unlimited maneuverability.
Diamond Plate Dolly Connector
The Diamond Plate Dolly Connector is perfect for detailers who use the two-bucket wash method. One bucket holds the soapy water. The second bucket holds clean water. Each time you need to reload the wash mitt with soap, first dunk it in the bucket of plain water to remove any dirt youÆve removed from the vehicle. Then reload the mitt with soapy water. Continue in this manner until youÆve washed the entire vehicle. YouÆll be surprised at how much dirt youÆve kept off the vehicle!
If you use the two-bucket system, you may already have two buckets with dollies, but you still have to move two buckets separately around the vehicle. Keep everything together with the Diamond Plate Dolly Connector. The Diamond Plate Dolly Connector simply connects the two dollies to keep your two buckets side by side, right where you need them.
The Grit Guard Dual Bucket Washing System is ideal for pro detailers and anyone who is serious about good detailing practices. The system makes the two bucket method truly convenient, and your vehicle will have fewer wash-induced swirls. By using this system, high quality products, and microfiber towels, you'll maintain smoother, swirl-free paint!
System includes:
2 Clear 5 Gallon Bucket
2 Grit Guard Inserts
2 Gamma Seal Lids
2 Grit Guard Dollies
1 Diamond Plate Dolly Connector
A total retail value of $139.97.
Save $19.98!
Additional discounts are not applicable.
Please call for a quote for next day air, 2 day or 3 day expedited shipping on this item.
Grit Guard« Dual Bucket Washing System - BLACK
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Grit Guard« Dual Bucket Washing System - CLEAR
| Grit Guard« Dual Bucket Washing System - BLACK |
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Stars based on
Spokane, WA
4 Stars
I would recommend this item to a friend.
Great Produce
July 30, 2014
I like that I can keep dirty from clean when I am washing my autos
rolling buckets--easier to move around autos
I think a lit5tle spendy for a couple of buckets on wheels--but very handy
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12 Brilliant Business Ideas Taken From Famous Rock Stars
Business is a lot more fun when you learn from rock stars instead of text books. If your ears are open, you'll find that many essential business lessons are coming through your speakers. Turn it up to "11" and sing along to the 12 Most Compelling Business Lessons from Rock Stars, adapted from the pages of the book "Brand Like A Rock Star: Lessons From Rock 'n' Roll To Make Your Business Rich and Famous."
Rock Star Business Lessons
1. Be as unique as KISS
There was nobody like them. They wore elaborate make-up, spit blood, breathed fire, and blew stuff up on stage. Because of that, they got noticed. Getting noticed is the first critical step in marketing. If you don't get noticed, you don't exist… and you'll never have the chance to prove to anyone how great you are.
2. Be as consistent as AC/DC
Every album has the same iconic font. Every picture of the band has Angus wearing a school boy outfit. And they never sing about starving children in the third world or the angst of a relationship that ends badly. If your business isn't consistent, people will never know what to really expect from you.
3. Be as hated as Nickelback
The whole world hates Nickelback. Yet they've sold over 50 million albums and continue to sell out concerts everywhere, every night. Nickelback knows that if just 10% of the population loves them, the other 90% don't matter. Having people hate your brand is a positive thing. It means they know you and understand what you're about. As long as you have people who feel just as strongly towards you, having haters rocks.
4. Be as PR-savvy as the Sex Pistols
They only ever had one album, but they made it into the Rock and Roll Hall of Fame because they shocked the world with their antics. Manager Malcolm McLaren carefully constructed chaos around his band everywhere they went, making the news at every turn. He knew that if people were talking about his band, they would come see his band and buy their records. He was right, and the same applies to your brand.
5. Be as gracious as the Grateful Dead
They gave away their music. Fans were invited to record and share the live shows. The Grateful Dead knew that spreading their music, even for free, would result in more ticket and album sales. It was a brilliant move and helped make them one of the top-grossing live bands of the 1970s and 80s. Does your company give something away in order to build a tribe of passionate followers? It doesn't have to be product. You can give away time, knowledge, and expertise.
6. Be as focused as Bob Marley
When Bob Marley started making music, the word "reggae" didn't exist. That's how small his niche was! He became one of the top-selling stars in music by being intensely dedicated to his craft. Bob Marley didn't make any other kind of music, and never compromised in order to reach more fans. Great brands have that same focus, clearly establishing a singular point of differentiation.
7. Be as human as The Beach Boys
Their 1965 hit "Barbara Ann" was full of mistakes. People were drunk in the studio, singing along to a song that they didn't know the words to. And the Beach Boys left them all in. The song connected with people because it was real… it actually sounded like a party-in-progress. When your business exposes some flaws, intentionally, it becomes human, allowing people to fall in love with your brand in an entirely new and personal way.
8. Be as brave as Johnny Cash
His career was over. When Johnny Cash went into the studio to work with hip hop and rock producer Rick Rubin, he was a long-since faded star. It took guts to work with Rubin, and to record covers of songs by Nine Inch Nails, Soundgarden, and other alternative rock stars. Johnny Cash went out on a limb, and the result was a series of albums that revived his career and cemented is status forever. Have the bravery to take a few calculated risks, like Johnny Cash did, and great things could happen.
9. Be more humble than Axl Rose
He spent 18 years and a reported $13 million dollars making the most expensive album ever recorded, Guns N Roses "Chinese Democracy". For 13 years we heard how epic this album would be, and when it finally came out it was a monumental let down. Yet musically, it wasn't that bad. It is remembered as a failure because of hype. Hype is empty. Instead of selling us empty hype, create honest anticipation for your brand.
10. Be as true as Bob Dylan
Bob Dylan did whatever he wanted. He went electric when everyone wanted him to sing folk songs. He recorded a Christmas album when people least expected it. Bob Dylan has remained true to Bob Dylan. Great brands have that sense of purpose. They have a set of internal values and they remain true to them, quickly finding out that there are millions of people who share those very same values.
11. Be as rare as Led Zeppelin
The 2007 Zeppelin reunion concert broke records for ticket demand. Over 20 million ticket requests were made for the 20,000 seats at London's O2 Arena, crashing servers instantly. Since breaking up after the death of drummer John Bonham in 1980, Led Zeppelin has rarely played together. When supply is that low, and demand is that high, the value of a ticket goes way up. There's nothing wrong with working the supply-and-demand equation in your favor. Keep demand high and make your product somewhat scarce, and you'll increase the perceived value for your brand.
12. Be an experience like Jimmy Buffett
Where can you see thousands of people wearing coconut bras and grass skirts, drinking margaritas? A Jimmy Buffett concert isn't about the music, it is about the unique experience. Great brands don't sell products, they sell experiences. Those experiences are what we are buying into. Give your customers a really cool experience instead of pitching them another product that they don't know they need.
Article Originally appeared on 12most.com
What a great article! I love point number three about Nickelback. So true. You don't need everyone in the world to love your brand.
Excellent and highly original article. Fun and very very true!


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Staying in Afghanistan
Why stay in Afghanistan if the West is not building durable institutions?
There is a growing chorus of right-of-center national security analysts warning that President Obama's Afghan pullout is going to end in disaster. While I don't agree with their prescription (stationing tens of thousands of troops in Afghanistan indefinitely) I do think they're right to warn about the deterioration in security that's likely to follow a U.S. withdrawal. Afghanistan may not revert completely to Taliban rule or abject disorder, but it seems foolish to blithely assume, as the Obama administration's public rhetoric suggests, that all is well on the path to a stable "transition" to Afghan control.
But this deterioration points to one of the underlying problems with stationing troops in Afghanistan forever. If ten years of U.S. and international efforts have not produced durable stability, why would ten or twenty more years do the same?
More broadly, the failure in Afghanistan points to a central issue facing U.S. and Western counter-terrorism policy in general -- how to deal with ungoverned spaces? The French intervention in Mali, the not-so-covert American campaigns in the Horn of Africa and Yemen, the drone war in Pakistan -- these are all ways of grappling with the threat without committing massive numbers of soldiers and financial resources to rebuild governing institutions. It's likely that Afghanistan will fall into this category as well after 2014. This template may not satisfy the neoconservative fantasy of Kipling 2.0, but it's hard to see a feasible alternative that won't quickly bankrupt already economically challenged governments.
(AP Photo)


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Product Description
New for Cordless Phones 900 MHz or more!
This large WaveShield 3000 is used for cell phones or cordless phones. Cordless Phone Radiation Protection EMF Shield's large diameter is need for lots of house phones and slightly larger cell phones. It's ability to stop cell phone radiation is just the same as all other models. The vast majority of electromagnetic radiation emitted by cellular and cordless phones comes from the antenna and parts of the phone other than the ear piece. Wave shield products have no effect on this electromagnetic energy. They may reduce the strength of electromagnetic-field emissions from cell phone ear pieces by as much as 50-97% depending on the cell phone model, without affecting the quality of the transmission.
- Fits all phone styles
- Attaches in seconds
- Soft/comfort cushion
- Will not affect reception
Without Protection
Electromagnetic radiation may enter the unprotected area of the inner ear when phone is in use.
With the "Wave Shield"
Electromagnetic radiation is blocked from the soft tissue of the ear canal when the "Wave Shield" is attached.
Additional Information
|Product Type
|Energy Tools


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Looking for a diamond shaped necklace? This necklace is perfect for you, it features a diamond shaped pendant in the outline of a diamond with the words "This is fake, I am not" etched onto it in silver! Totally cool and unique! We have more diamond shaped jewelry in our store!
Size: 1.5 inches tall charm that hangs on a 28 inch (72 cm) long chain
Material: Silver Plated Brass
Unique and Affordable Jewelry by DOTOLY
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East End opens up with new roadway running clear
April 28, 2012 by Grace Franklin · 1 Comment
The £25 million Clyde Gateway (the East End Regeneration Route) opened to traffic on Thursday 26 April 2012. It is a key piece of infrastructure associated with Clyde Gateway Urban Regeneration Company and the Glasgow 2014 Commonwealth Games and the expectation is it will bring jobs and economic advantage to the East End of Glasgow – Shawfield and Dalmarnock in particular – by improving accessibility.
The four-lane, 2.6km carriageway links the Oatlands and the M74 junction at Polmadie in the south to the Forge Retail Park in the north. Designed by Gronmij and built through a joint venture between Farrans and I&H Brown, it will give easy access to Celtic Park, where the opening ceremony of the Commonwealth Games will be held, the Commonwealth Arena, Sir Chris Hoy Velodrome and the Athletes' Village.
This will be a critical route to transport some of the 18,000 athletes and support staff and hundreds of thousands of spectators expected during the Games. Afterwards, the roadway will be a legacy for the benefit of the local community.
Traffic congestion on existing local roads should also ease, especially during peak times. Recent traffic modelling studies have shown that there will be a reduction in traffic across the major east/west arteries crossing road around London Road and Gallowgate and in association with the new M74 link, this will free up road space to allow for additional walking, cycling and bus routes to be put in place.
Phase 1 of the road opened in April 2007 as part of the development of new housing in the Oatlands area and was officially re-named- New Rutherglen Road. Phase 1A followed in April 2010, running from the Polmadie junction of the M74 and Shawfield Stadium. This stretch totals 1.5km.
Phase 2 is the longest section, crossing the Clyde at Rutherglen Bridge and passing Dalmarnock Railway Station, the Commonwealth Arena and Celtic Park before joining the Parkhead by-pass at the Forge Retail Park
Brian Devlin, Executive Director for Land and Environmental Services said: 'The Clyde Gateway creates a new, direct link between the completed M74 and the heart of Glasgow's East End. This will offer fantastic new opportunities for people and business either currently living or based in this part of Glasgow or looking to move there. This is part of the wider regeneration of the city.
Neil MacDonald, Chairman of Clyde Gateway Urban Regeneration Company said: 'The M74 has already shown that new roads play a very important part in businesses choosing where to make crucial investment decisions and there is no doubt that Shawfield and Dalmarnock in particular will benefit from this new piece of infrastructure. Our on-going efforts to attract developers to the East End have been helped immensely by this road opening and I'd like to thank Glasgow City Council for again demonstrating their commitment to the long-term regeneration of the Clyde Gateway area.'
Prior to the formal opening when traffic started flowing, children from four primary schools in the east end were given the chance to try out the newest section of the road.
More than 100 Primary 6 and 7 pupils from St Michael's, St Anne's, Dalmarnock and Quarrybrae primary schools cycled around an obstacle course set up on part of the new tarmac running from new Oatlands over Rutherglen Bridge, through Dalmarnock to Gallowgate.
The children experienced, first hand, the road's new cycling facilities including dedicated cycle lanes and extended footpaths that are provided along the full length of the route.
They also got the chance to brush up on their safety skills with Glasgow City Council road safety officers and Strathclyde Police cyclists and motorcyclists. Dr Bike offered advice on maintaining bikes and gave practical demonstrations to ensure they were fit for the road.
As well as providing better facilities for cyclists, the new road, funded entirely by the Council, will improve public transport links and accessibility around the East End.
With phase one opened as part of the Oatlands new neighbourhood development last year, phase two of the 2.4km stretch runs over Rutherglen Bridge, continues via Dunn Street, Poplin Street, Dalmarnock Road, Mordaunt Street, London Road and Camlachie to join the existing road network at the Parkhead by-pass, Forge Retail Park.
TheClydeGateway (Phase 2) Facts
1. More than 35,000tonnes of asphalt used to lay roads, footpaths and cycle ways.
2. 800m of 2.74m diameter tunnel used to alleviate storm water flooding.
3. More than 250 trees planted and 40,000 sq m of landscaping to the road corridor.
4. 10km of new drainage pipes installed for new roads.
5. 250 new traffic signal heads installed over seven junctions.
6. 330 new lighting columns.
7. Construction period 2 years (April 2010 to April 2012)
Pram fire takes 13 to hospital
December 23, 2011 by Grace Franklin · Leave a Comment
Thirteen people were hospitalised after a pram in a close was set on fire. All were later released after being treated for varying degrees of smoke inhalation.
The incident, in Calder Street on the city's Southside, highlights the dangers of storing anything in common areas.
Polmadie and Castlemilk fire crews wearing breathing apparatus, quickly extinguished the minor blaze. But it generated an extremely large volume of thick smoke which quickly rose to the top of the four storey tenement.
Alarmed residents on opening their doors to check on the commotion in the building, breathed in the potentially dangerous fumes. As fire service personnel brought residents out of the building, ambulance and police ensured everyone was taken to safety, quickly.
Strathclyde Fire and Rescue personnel emphasise that especially at this time of year with Christmas decorations and parties, they will come to give a free Home Fire Safety Visit at an agreed time.
Experience fire crew assess the individual home and offer fire safety advice tailored to that property and the people living there. This can include the fitting of smoke alarms.
McFrankenstein tackles the Missing Link.
May 19, 2011 by Grace Franklin · Leave a Comment
Up to 20,000 people are expected to take part in the M74 Bike 'n' Hike event on Sunday 22 May. Before the 'missing link' stretch of motorway from Shields Road to Polmadie to Fullarton Roundabout is opened to traffic, the M74 Project Partners and Contractor have agreed to let pedestrians, cyclists and runners cover the whole 14km stretch or parts of it, as people are able.
If you spot a cyclist dressed as McFrankenstein (Frankinstein with a Scottish twist) cheer him on and support him as he's doing the whole 14km in aid of Yorkhill Children's Foundation.
'So far, I've raised around £200,' said McFrankenstein who is known to his friends as Colin Todd.
'I have my own Facebook page for the event: http://www.facebook.com/#!/pages/Colin-Todd-M74-Bike-n-Hike-event-in-aid-of-Yorkhill-Childrens-Foundation/209718129061513. It gives a link to my Just Giving website too,' said the care worker. He has done several similar fund raisers – including the route between Glasgow and Edinburgh in the Pedal for Scotland event. 'A friend's son was in intensive care for three weeks after he was born so I knew, though his experience, and his ongoing care, how important the Yorkhill Children's Foundation is. And like to support it when I can.'
People can register up till noon on Friday 20 May to walk, run or cycle the route. The event will start from the Shields Road Car Park in Scotland Street. The road formally opens in June.
Further information from : www.yorkhillchildrensfoundation.org and
Colin's Just Giving site: www.justgiving.com/Colin-Todd0/
Oatlands group to discuss St Margaret's
November 17, 2009 by localnews · Leave a Comment
The next meeting of the Oatlands Steering Group, on 3 December, will discuss the future of the former St Margaret's Polmadie Church and its conversion into a community facility.
Residents and 'stakeholders' are encouraged to participate in a St Margaret's community working group. Those interested will be asked to take part in a workshop to look at the latest design proposals and uses for the building; to discuss ways the community can be involved in running the facility in the long-term and training initiatives which will enable this to happen; to visit other sites which share similarities with St Margaret's, and to set up and attend regular meetings.
The meeting will take place at Oatlands Community Resource Centre (the Blue Hut) Wolseley Street, Glasgow, at 2pm.


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India Inc's business confidence high with Modi govt in power
Jun 12 2014 , New Delhi
The survey of 300 entrepreneurs belonging to the PHD Chamber also indicates that the momentum of doing business in India would accelerate in the near future.
"Over 80 per cent of business people reasoned that Modi's leadership spurred stock market boom and brought back confidence at higher echelon of bureaucracy for expediting decision making with his assurance that consequential after effects noticed later on in their decision taken for serving the public cause will not be subjected to scrutiny," President of PHD Chamber Sharad Jaipuria said.
Vowing to control inflation, Modi said yesterday in his address to Parliament that improving agriculture productivity through modern farm techniques and university-farmer collaboration hold the key to check price rise.
Emphasising the importance of increasing farm productivity, Modi said there is a need for use of modern technique in the agriculture sector because farm land is shrinking with rising population.
The Prime Minister talked about his "dreams" of ensuring the country's progress and converting the image from "scam India" to "skills India" through "cooperative federalism".
Meanwhile, The benchmark Sensex rebounded 102.32 points to end near record highs led by HDFC and HDFC Bank shares ahead of industrial output and retail inflation data releases due later today.
The BSE 30-share barometer resumed higher and moved side ways in a range of over 200 points before ending at 25,576.21 -- a rise of 102.32 points or 0.40 per cent.


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Your Cat, Your Life
The Scoop
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Your cat loves a clean toilet -- don't we all? Catster's singing Cat Lady, Sarah Donner, explains why.
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Last night I was trying to play with my brother, but he didn't want to play, so mom scooped me up and told me she was going to take me to get something fun. She reached into a bag and pulled out a ball with a string attached to it. She told me that it was a ball of yarn she no longer needed and I was allowed to play with it. What joy! Before I knew it, I had it all over the stairs and the landing and wrapped around myself. This thing is great! Since mom gave it to me, I don't let my brother near it. He tries to play with it when I'm not looking, especially when I'm eating, but I see him and pounce on him. I was so thrilled by my new toy that I didn't have any time last night to pull down the rope caulk from the window in our bedroom like I normally do at night (yes, we cats have our own bedroom). Mom told me to be good today, so I'll try...key word is try.
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|Thread Tools
I've got the manual, and I've tried to ingest it all, but frankly, many of the features they describe are things I don't quite understand.
I'm very happy with my receiver, but there are a couple of nuisance items I've tried to google, and just can't seem to get the search phrasing right.
1. The "network" mode lets me listen to spotify (A+). But.... it also disables my primary display (F). I would like to have my primary display (computer desktop) hdmi video still go through, even if the sound is dropped. There is a "passthrough" option, but that seems to be unrelated to what I'm trying to accomplish. Is there a way to do it? (if you are wondering, the spotify on my PC doesn't get full range, no matter what I tried to do, there's no low range bass. According to the internets, it has something to do with a built in signal equalizer baked in to the spotify app. Allegedly I can fix it by running xmbc or something.... I gave up on it.)
2. Why does my hdmi1 input (PC) flash off, and back on, when I plug in my phone to the dock? (connected to hdmi port 5) Are ports 1(bd/dvd) and 5(aux) linked somehow?
Other components don't seem to do this when powering off/on (ps4/xbox360/ps3). Bonus question, why does my phone (galaxy note II) refuse to shut off the display when it's docked (samsung smart dock) and connected via hdmi? (I have to push the button to force the screen off, it won't time out).
P.S. as I've googled around, many places have stated that the onkyo 758 is really a 414. They look similar, and they seem to share firmware, but the 758 has audyssey and all sorts of dolby this and dts that sorts of things that the 414 doesn't, and it's got 4k upconversion etc, so I just wanted to point that out in case anyone tries to help me, and gets tripped up by the 414 comparisons.
Thanks for reading at least, if you've made it this far.
Maybe you might not know anything about my specific receiver, but at least for the first part, maybe someone can tell me if what I'm suggesting sounds like something that it *should* be able to do, or maybe you have had a lot of experience with onkyo in general, and it doesn't sound like any sort of feature you've seen/heard of before.
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An entry for the "Inspired By A Song" contest. Inspired...
Wonder (Poetry Collection, Part II)
- by RebeccaHarrisWriting
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- Published: 14 Jul 2016
- Updated: 18 Jul 2016
- Status: In Progress
"Do you suppose she is a wildflower?" - 'Alice in Wonderland', Lewis Carroll. Poetry centering around the madness of love, understanding yourself, and dreaming, part two of a poetry collection. Inspired slightly by Lewis Carroll's 'Alice in Wonderland'.
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Project Design
The importance of the project design phase of a genomics experiment cannot be underscored enough. The core units under the Gene Microarray Shared Resource (GMSR) are committed to supporting an investigator's experimental vision even before the project application process. In addition to reviewing the project design resources on this website, contact the GMSR Core Director, Dr. Chris Harrington, for project consultation (no fee for standard level of support).


text: ntegrating breathing techniques, Liu Tong Exercise offers relaxation as well as building up physical strength and improving muscle tone. — The set of 36 movements or postures is easy to learn. Patients with physical impairment can do it sitting down. It does not require special outfit or equipment. Once you have learned it, you can do it almost anywhere at any time. The benefits are many. Participants report better balance, better sleep, better mood, less stiffness and less pain. In 1996, Liu Tong Exercise was introduced at Pacific Complementary Medicine Center by Bob Hong. Liu Tong Exercise consists of 36 movements, each specifically designed to stimulate a certain section or regulate a certain function of the body. These postures are surprisingly simple, yet they call for a certain precision of form to bring the desired results health-wise. Each is repeated 10 times in a rhythmic motion. Throughout, consistent repetition is basic to success. Age is irrelevant in the practice of Liu Tong. In a class where the age span was 55-92, all the students were able to go through the exercises, and did so with enthusiasm. It is helpful to have a gentle warm-up period, such as the building of chi (or qi) by rubbing the hands together, limbering the shoulders with swimming motions and deep abdominal breathing. Of course, deep breathing is a factor in the entire program, but, in the warm-up, it is done consciously as an exercise unto itself.


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Is Porsha On That Ish?
Did drugs and alcohol cause Porsha Williams to go nuts on Kenya Moore? Sources tell Radar Online "Porsha was drunk… there were two bottles of wine in her dressing room. She came to fight Kenya and needed some liquid courage!" What's more, the source alleged that Williams has been taking prescription drugs to deal with depression following the divorce from ex-husband Kordell Stewart, as well as painkillers following her recent breast augmentation and they did not mix well with her beverage of choice."She is very unstable," the source alleged. "She's been taking medications and mixing it with alcohol." "Bravo knows that she is now nothing but a liability," the source added. "Her attack on Kenya failed, and she lost her mind. It's all very sad."
Photo Credit: Getty Images


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Methods for Detecting Protein-RNA Interactions
RNA Electrophoretic Mobility Shift Assay
The RNA-EMSA is an in vitro technique used to detect protein-RNA interactions through changes in migration speed during gel electrophoresis. First, a labeled RNA probe is incubated with a protein sample (typically from a cell lysate) to initiate binding and formation of the interaction complex. The binding reaction is then separated via non-denaturing polyacrylamide gel electrophoresis. Like protein-DNA complexes, a protein-RNA complex migrates more slowly than a free RNA probe through a gel matrix. This causes a migration shift relative to the nonbound RNA probe. Specificity is determined through a competition reaction, where excess unlabeled RNA is incubated in the binding reaction, resulting in a decrease in the shifted signal if the labeled and unlabeled RNA sequences compete for binding of the same protein. Alternatively, the protein-RNA complex may be crosslinked and the reaction run on a denaturing gel. Specificity is determined through visualization of a single shifted band. Traditionally, RNA probes are radioactively labeled for detection, although fluorescent and chemiluminescent detection is also possible. Non-radioactive RNA end-labeling techniques are limited, but more versatile biotin and fluorescent labeling methods are now available.
Protein Interactions Handbook
Our 72-page Protein Interaction Technical Handbook provides protocols and technical and product information to help maximize results for Protein Interaction studies. The handbook provides background, helpful hints and troubleshooting advice for immunoprecipitation and co-immunoprecipitation assays, pull-down assays, Far-Western blotting and crosslinking. The handbook also features an expanded section on method to study protein:nucleic acid interactions, including ChIP, EMSA and RNA EMSA. The handbook is an essential resource for any laboratory studying Protein Interactions.
Contents include: Introduction to Protein Interactions, Co-Immunoprecipitation, Pull-Down Assays, Crosslinking Reagents, Label Transfer, Far-Western Blotting, Protein Interaction Mapping, Yeast Two-hybrid Reporter Assay, Electrophoretic Mobility Shift Assays [EMSA], Chromatin Immunoprecipitation Assays (ChIP), Protein:Nucleic Acid Conjugates, and more.
RNA Pull-down Assay
RNA Pull-down Assays selectively extract a Protein-RNA complex from a sample. Typically, the RNA pull-down assay takes advantage of high affinity tags, such as biotin or azido-phosphine chemistry. RNA probes can be biotinylated, complexed with a protein from a cell lysate and then purified using agarose or magnetic beads. Alternatively, the protein may be labeled, or the RNA-Protein complex may be isolated using an antibody against the protein of interest. The RNA is then detected by Northern blot or through RT-PCR analysis and the proteins detected by Western blotting or mass spectrometry.
Oligonucleotide-Targeted RNase H Protection Assays
RNase protection assays (RPA) are a powerful method for detecting RNA and RNA fragments in cell extracts. Unlike Northern blotting or RT-PCR analysis, RPA assays allow greater flexibility in the integrity of target RNA, requiring very short segments for hybridization and detection. RPA assays can also be used to map protein-RNA interactions. In this adaptation of the RPA, RNase H is used to cleave a target RNA molecule at a specific site hybridized with a DNA probe. If a protein is bound to the RNA at the target sequence, it will prevent will block probe hybridization, prevent cleavage by RNase H and indicate a site of interaction between protein and RNA. RNase H requires only a four basepair hybrid with a DNA probe in order to cleave the RNA molecule of interest. Using many small probes allows the entire sequence of RNA to be mapped for sites sites of interaction.
Fluorescent in situ Hybridiation Co-localization
Fluorescent in situ Hybridization (FISH/ISH) co-localization techniques require detection of both a RNA transcript and a protein of interest using RNA probes and antibodies. FISH/ISH detects the position and abundance of a RNA and protein in a cell or tissue sample. The read-out is visual (usually imaged via microscopy) and a co-localized signal for both the RNA and protein of interest indicate possible complex formation. A labeled RNA probe must be generated for detection of a particular sequence of RNA and the protein may be detected using antibody staining or fluorescent protein constructs.
Selected References for Studying Protein-RNA Interactions:
- Friedman, R. C., et al. (2009). Most mammalian mRNAs are conserved targets of microRNAs. Genome Research 19: 92-105.
- Cannell, I. G., et al. (2008). How do microRNAs regulate gene expression? Biochem. Soc. Trans. 36: 1224-1231.
- Mendes, N. D., et al. (2009). Current tools for the identification of miRNA genes and their targets. Nucleic Acids Research 37: 2419-2433.
- Eulalio, A., et al. (2008). Getting to the root of miRNA-mediated gene silencing. Cell 132: 9-14.
- Datta, J., et al. (2008). Methylation mediated silencing of MicroRNA-1 gene and its role in hepatocellular carcinogenesis. Cancer Res 68: 5049-5058.
- Meister, G. (2007). miRNAs get an early start on translational silencing. Cell 131: 25- 28.
- Su, H., et al. (2009). Essential and overlapping functions for mammalian Argonautes in microRNA silencing. Genes & Dev. 23: 304-317.
- Farazi, T. A., et al. (2008). The growing catalog of small RNAs and their association with distinct Argonaute/Piwi family members. Development 135: 1201- 1214.
- Tu, K., et al. (2009). Combinatorial network of primary and secondary microRNA-driven regulatory mechanisms. Nucleic Acids Res. 37: 5969-5980.
- Pesole, G., et al. (2002). UTRdb and UTRsite: specialized databases of sequences and functional elements of 5' and 3' untranslated regions of eukaryotic mRNAs. Update 2002 Nucl. Acids Res. 30: 335-40.
- Pesole, G., et al. (1997). Structural and compositional features of untranslated regions of eukaryotic mRNAs.Gene 205: 95-102.
- Klausner, R.D., et al. (1993). Regulating the fate of mRNA: the control of cellular iron metabolism. Cell 72: 19-28.
- Ying, B., Fourmy, D., and Yoshizawa, S. (2007). Substitution of the use of radioactivity by fluorescence for biochemical studies of RNA. RNA 13: 2042-2050.
- Gunzl, Arthur and Bindereif A (1999). Oligonucleotide-targeted RNase H protection analysis of RNA-protein complexes. Methods Mol Biol. 118:93-103.
For Research Use Only. Not for use in diagnostic procedures.


text:
Canada demands oil trains carry more insurance, levy imposed
By Randall Palmer
OTTAWA (Reuters) - Canada will increase the insurance railways must carry when they haul crude oil and impose a levy on shippers to help cover the cost of major accidents in the burgeoning oil-by-rail industry, Transport Minister Lisa Raitt said on Friday.
Raitt unveiled the measures in a bill which responds largely to the 2013 explosion of a runaway train that leveled the heart of the Quebec village of Lac-Megantic and killed 47 people.
The legislation also gives her department more power to step in if it feels a railway is not being run safely.
"Rail companies will be concerned about the amount of extra regulation that we may be putting in place today, but for the safety of Canadians and protecting communities it is the right way to go," Raitt told reporters.
With demand for pipelines outstripping capacity, crude is increasingly being shipped on long trains, and Raitt has put a priority on tightening safety and imposing the polluter-pays principle for accidents.
The costs of the cleanup and reconstruction for Lac-Megantic far exceeded the C$25 million ($20 million) insurance carried by the Montreal, Maine & Atlantic Railway, driving the railroad into bankruptcy and leaving Canadian federal and provincial governments to pick up the tab.
Within two years federally regulated railways carrying any crude oil will now have to have insurance of at least C$100 million, ranging up to C$1 billion per incident for annual shipments exceeding 1.5 million tonnes.
The rules cover the Canadian operations of U.S. firms such as BNSF Railroad, which is owned by Berkshire Hathaway Inc, CSX Corp and Union Pacific Corp. Continued...


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My wife and I recently spent a day at the Kimbell Museum in Fort Worth. In the permanent collection is what may well be Michelangelo's earliest painting, "The Torment of St. Anthony" --- a vivid portrayal of St. Anthony's struggle with temptation in the desert. It got me thinking...Our inner lives are too small.They are cluttered with the kind of distractions that they sell in tourist traps --- pot metal and plastic, cheap construction, loud colors --- here today, gone tomorrow, about to b … [Read more...]
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Springer Protocols
Springer protocols is a database of reproducible laboratory protocols in the life and biomedical sciences offering researchers access to nearly thirty years worth of time tested, easily reproducible, step-by-step protocols for immediate use in their lab. Includes protocols from the Humana Press series: Methods in molecular biology, Methods in molecular medicine, Methods in biotechnology, Methods in pharmacology and toxicology, and: Neuromethods.
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By Stayson Isobe, NAU Media Relations
FLAGSTAFF, Ariz. – It is no secret that the sport of soccer in Flagstaff is deeply intertwined. At the heart of Flagstaff soccer resides the Northern Arizona women's soccer program and over the last few months, a new chapter has been added with 2012 alums Jenna Samora and Natasha Slaughter lending their heart, hands (and feet) to local Flagstaff High School.
Without a head coach after its previous coach stepped down from the position, the Flagstaff High School varsity girl's soccer team was in desperate need. Samora was one of the players' favorites to take over the Eagle squad having coached many of the team's players as part of the Flagstaff Soccer Club. After a first moment of hesitancy, Samora eventually took on the head coaching duties the day before try-outs in the fall and the fit has been a perfect one for the Lumberjacks' all-time career leader in games played and fourth all-time leading goal scorer and point totaler.
"It's been an amazing experience," Samora said. "I'm so lucky to get this opportunity as a young coach to come and be the head coach of a high school team. It's been a fairly easy transition since I've coached so many of these girls in club, but it has been a bit of an adjustment with all of the tradition here."
The tradition that Samora speaks of is rich at Flagstaff High School. The recent run of success began in 2007 when Holly Jones, now an assistant coach at NAU where she still holds the school and Big Sky records for goals scored and points, led FHS to its first of back-to-back state championships. After a runner-up finish in 2010, the Eagles reached the pinnacle for a third time in five years, claiming the state title in 2011. For some help, Samora tapped into that history in the form of Slaughter, her teammate at NAU.
Slaughter, a native of Flagstaff, knows a thing or two about soccer at Flagstaff High School having served as the goalkeeper on the Eagles' first two championship teams in 2007 and 2008. As Samora's assistant coach on the varsity team and the head coach of the junior varsity team, Slaughter is right back to where it began for her – at her alma mater.
"It's been nice to come back here," Slaughter said. "To go from a player to a coach is a nice change of pace. It's different to see how things have changed, but there are some traditions that I still remember when I was a player here. I've really liked it so far."
Samora follows a long lineage of former Lumberjacks that have gone on to become high school coaches in town beginning with Jones, who led the girl's soccer program at FHS from 2001-08 and guided them to its first two titles. During her tenure, her assistants included former Lumberjacks Lisa Van Gorder and Kim Morrow. Another former NAU standout, Krista Earp, was the head coach at Sinagua High School.
For NAU head coach Andre Luciano, who coached all of the previous players with the exception of Earp, he's proud to see Samora and Slaughter continue the legacy set by those before them in the community.
"I'm really proud to have them work with the local high school," Luciano said. "It's a tradition that we've passed along since Holly took over at FHS and we hope to continue to give back to the community in any way possible whether they are teachers or coaches."
Even though Samora and Slaughter are both recent graduates and are coaching at the high school level for the first time, this is not their first go-around at sharing their knowledge of the game with the Flagstaff youth. Both got their start volunteering at the NAU soccer camps and later took on extensive roles in the Flagstaff Soccer Club. Currently, Slaughter serves as an assistant coach with the U11 girl's team and works with some of the U17 and U18 goalkeepers. In addition to her head coaching duties at FHS, Samora is also the head coach of the U18 team, which she took over for Luciano, and the U9 team as well as being an assistant with the U16 team.
"Jenna has a soccer purist mindset and she is extremely passionate about the game," Luciano said. "Her professional background is going to be teaching, so I thought it was a natural progression for Jenna to get into, and that's why I passed on my oldest Flagstaff Soccer Club team to her. I was surprised to hear about Natasha, but she developed a passion for coaching from the soccer camps and I'm glad she followed through with that. A lot of credit though has to go to Holly for getting them involved with the club."
With Samora and Slaughter at the helm, both of whom are quick to credit Luciano and Jones for teaching them the little things about coaching, the Flagstaff High girls entered the Division II state playoffs as the No. 2 seeded team following a 12-0 regular season in which they outscored their opponents by an 81-4 margin. The Eagles notched their 13th win on Tuesday, shutting out No. 15 Mohave, 10-0, and will next host No. 10 Notre Dame Prep on Saturday at 2 p.m. in their second step towards the quest for a fourth state championship in seven years.
"The season has gone really well, but I don't feel they've been pushed to their max," Samora said. "They've done a great job of taking care of those games and preparing for the state tournament with what they've been given. This is an amazing group of girls and they make this job easy. I hope to keep working with them for another three games (to the championship game)."
With that, the tradition at Flagstaff High School lives on and the story of soccer in Flagstaff continues.