text:
I had a good time at MAX, learned a few new things about some Flash Player 10 internals, learned some new things about performance tuning from Sean Christmann, and found out that there's a lot of stuff going on with getting the Flash Player to run on ARM, which you should read as "on powerful Mobile phones", which you can read however you would like.
I'm also going to lay out a quick note of how I solved the "AIR communicate with the System" problem. I wrote a wrapper application that launched the AIR app when it was started up, nothing new or exciting there. Then I just wrote commands that looked like:
"ls -a {0}"
where I would just fill {0} with the value passed from the AIR app. I encrypt those so that the command can't be tinkered with and unencrypt them at runtime. Then, in a very lightweight (i.e. ~4kb) process I just check the file to see if it exists and whether it's locked, if it's not locked, then we read it, fill the command with the parameters, and then pass it to the system to be executed.
The meat of the OSX version of this looks more or less like so:
// this is the threaded method
void *process( void *data )
while(run) {
char buffer[1024];
sprintf(buffer, "rm -f %s", f->cfile);
return (void*) 1;
int main(int argc, char *argv[])
char* lockFile;
char* commFile;
char* result;
printf(" starting up ");
commFile = (char*) malloc(1024 * sizeof(char));
lockFile = (char*) malloc(1024 * sizeof(char));
result = (char*) calloc(1024, sizeof(char));
pthread_t thread;
int threadi;
// you'll want to encrypt your system commands just in case
// so you could do
// xorUnencrypt(addToSafe);
// start up the air app, if it works we'll create the thread
if(startAIRApp(commFile, lockFile) == 1)
run = true;
// thread identifier
threadi = pthread_create(&thread, NULL, process, NULL);
// go ahead and make the thread
pthread_join(thread, NULL);
Is this beautiful C? Nah, not really. But it works. The rest of the OSX version (extremely sanitized for the sake of my future employment) is here.
The hardest part of all of this was finding where the app wasn so that I could launch it. On OSX that requires that you make use of the FSRef object. Essentially, you do the following:
1) create a FSRef object
2) pass is to LSFindApplicationForInfo along with the name of the bundle from the plist file of your app
3) call FSRefMakePath along with a string that the path can written into
that looks pretty much like so:
OSStatus err;
FSRef appRef; // this is the OSX application object that you can get the app path from
char *appPath = (char*)malloc(1024); // just go ahead and do the max
err = LSFindApplicationForInfo(0, CFSTR(TARGET_APP_BUNDLE_ID), NULL, &appRef, NULL);
if (err != 0) { return err; }
appPath = NULL;
// note the wierd cast, ick
err = FSRefMakePath(&appRef, (UInt8*) appPath, appPathSize); // now your application path is written to appPath
And that's really all there is to it.


text:
|Topic Review (Newest First)
Very interesting tread on a very important question !
Anyway, to add a line of thought :
I remember reading a few years ago about a frenchie who rigg a scuba tank to a pvc pipe to throw some molotoff cocktails at incoming pirates !!
I am pretty sure that our american friends with their unparrallel knack with weapons will find us something in this line of thought
After all most cruising yacht have scuba tanks on board and they are not yet designed as weapons
So something using scuba tanks compressed air to throw a lethal projectile ??
Up to your drawing board
|I know we opted not to carry that Taurus, thinking a normal revolver loaded with "snake shot" would be a lot better than the Judge. It is almost as much of a flop as the Patriot pistol, which was a handgun made to fire the .223 or 5.56mm NATO round. It looked cool with lots of flame coming out every which way but would malfunction after 1 or maybe 2 rounds. I wouldn't plan on reloading, since rimless cartridges and revolver type extractors have never gotten along well. At least with a revolver you will get all 5 rounds going downrange. Since those countries that ban firearms are Draconian about ammunition as well this one you are going to be left with an expensive flare gun.
THE STEEL DEAL: Here come da THE JUDGE.
Is this what you're looking for?
|Cool, I'd forgotten about ISG's (Improvised Spud Guns)! Attach a little laser pointer and at 50 yards it looks enought like a RPG launcher that most unarmed pirates would probably opt to reciprocate bearings!
Pass the spuds
Got propane? If not, deodorant or hairspray will work. Ammo is pretty flexible too. Check this out.
Disassembed, it could pass for a (large) manual bilge pump.
How about a soda to go with those fries? Video showing 20 oz soda bottles going thru 3/4 inch plywood like it was paper. http://spudtech.com/movies/asf/megalauncher6.mpg
Originally Posted by Zanshin
To get back to boats - if I had a flare gun adapter that I had to use in self defence it might unfortunately fall overboard afterwards. No loss, I'd have something to add to the forum thread of offerings to Poseidon.
Another assumption would be that if I killed or injured a pirate out of sight of land, I'd be kicking his body over the side for either his buddies or the sharks to deal with. It's not the sort of episode I would care to mention to the authorities in the sort of countries that have a pirate element anyway. Captain's prerogative.
Originally Posted by hellosailor
don't forget that in England now there is a movement afoot to make pointed knives illegal...
The worries that are fuelling public sentiment here are not pocketknife folders (i.e. BenchMade/CRKT and the like) but what are termed "Stanley knives" - basically carpet cutter disposable blades bought at home improvement stores or the like. Some recent very public programmes have offered immunity for those disposing of knives and target youths. The legislation that has been passed does not prohibit carrying knives (although some have interpreted it as such, for which the police are happy) but does limit the blade length to 3" for folders. And, while legal, the police will detain and question anyone carrying a knife. I carry a clip-on pocketknife, and an officer told me that if I were stopped at night in the street and found carrying I would probably be taken into custody and questioned. My answer of "But officer, I've been carrying a pocketknife for 20 years" would be sufficient but they are counting on the nuisance value to deter people.
Although the bar scene in the UK is full of raucous and obnoxiously aggressive drunks, particularly around closing time, the level of violence outside of that is pretty low. One acquaintance in London who works in drug enforcement was telling me of a raid they did on a mid-level drug dealer. The only additional weaponry they had (i.e. no firearms) was a large mallet to beat down the door. In the U.S. you'd have a stick of armoured officers in full chem gear armed with full-auto subguns for the same scenario. Very different cultures!
To get back to boats - if I had a flare gun adapter that I had to use in self defence it might unfortunately fall overboard afterwards. No loss, I'd have something to add to the forum thread of offerings to Poseidon.
I find myself in the rare position of agreeing with two posters, with conflicting opinions, at the same time. Probably never happen again.
I do think it folly to lock up individuals with small amounts of drugs, and I do favor legalization and regulation as a means of "ending" the problem. But I also acknowledge the current illegality. If I am caught speeding, I pay the ticket regardless of the fact that the road I am travelling was designed for 80 mph speeds for cars built 50 years ago. I also resent the implication that no harm is done by the possession of personal use amounts of drugs. I possess personal use amounts of tooth-paste and an entire industry is supported by like minded people such as myself. If the penalties for 80 mph in a 70 mph zone reach incarceration I will, no doubt, modify my behavior and probably fire my legislator. The law may well be an ass, but it is the law.
|Don't take a knife to a gunfight. It's still good advice.
Zanshin, don't forget that in England now there is a movement afoot to make pointed knives illegal. That's right, ordinary kitchen knives are being used in hackings and stabbings and since there's really no need for a pointed knife in the home, there's a nicely organized movement in England to get these devastating and unnecessary weapons banned. No more production, and pehaps blunting the ones that are allowed to be kept.
I refuse to comment on the sanity of the group members, but suggest that the million Hutus and Tutsis who were hacked to death mainly with machetes (production cost, ten cents each, sale cost, $1 each) are proof that edged weapons still are in business.
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text:
Well Pump replacement- ripoff or not?
Here is a weird question about well pump replacement.
I would like to understand what is the opinion- was I ripped off or just overcharged.
Here are the facts.
I had kind of random loss of pressure lasting for 1-10 minutes.
I called a company that has worked on my well before.
The guy who came was not all that competent- he changed the connection box, which naturally did nothing. He asked for "backup" and the guy who came (and was competent) said it is the pump. This visit was 340 bucks.
I asked the first guy about the price and he said around 1500, not more than 3000. Silly me did not ask for written estimate. But they have worked before and changed the pump 3 years ago for 1500 so I was assuming there is nothing to worry about.
Next day a truck comes and they pull the pump, change it, all great... Until the bill came at 4344 dollars. Laber came to 1278 (part of the total 4344). So to have my well fixed- almost 4700 dollars.
Including 180 ft 12/2 wire at 531 dollars, Pentec 3/4 HP pump at 1035, electrical tape at 13 dollars, etc, etc. You get the idea. Everything was marked up by 3-5 times.
I confronted their supervisor and he declined that they ever worked on the house before or that they gave me estimate for "no more than 3000 dollars".
While the finished their job and everything seems OK so far, I have the feeling that was being not just overhcarged, but probably simply ripped off.
I do not want to be unfair to these people and this is why I want to get the opinion on people on the other side- do you think this is a fair price? Perhaps I do not understand everything that goes into this...
You did not want an "estimate", you wanted a "contract" with a "not to exceed" price, or perhaps a "firm fixed price" contract. Getting expensive work done without a written contract is always dangerous, I am not sure what you can do about it now. For what it is worth, I had a well pump replaced about ten years ago. Cost me about $2000, 250 foot deep well. I do not know what current pricing would be.
Wow,that is a scary number--last pump I had installed was 12 years back--$1500 as I recall--
No new wire or pipe was needed---180 feet deep--
Sounds like a Roto Rooter bait and switch they pull that stunt all the time:furious:. we proposal everything we do no surprises to the customer no misunderstandings upon completion. :wink:
Why do you need new wire ? They ripped you off big time.
39 Attachment(s)
I'd file a complaint with someone. There's got to be a law against making that much profit on materials. 10% -30% is normal. Not 300%
Thanks everyone!
Thanks everyone. I just do not want to be the grumpy customer who does not understand how much work is needed. Been on the other side for other things...
I am launching complaint with BBB- that is all I can do now...
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text:
Despite lip service, Silicon Valley venture capital still a man's world
By Sarah McBride
SAN FRANCISCO (Reuters) - When she started her child-support tracking business SupportPay, Sheri Atwood expected all kinds of suggestions - but not the tip she got from a female investor who suggested she dye her blonde hair darker to be taken more seriously by venture capitalists.
To Atwood, who eventually won her funding from other backers, the recommendation underscored an attitude in Silicon Valley that women make second-class entrepreneurs. If more women held the purse strings at venture capital firms, the attitude would change, she said.
Despite the lip service Silicon Valley has given over the past couple of years to the need to recruit more women venture capitalists, at senior levels the industry's gender balance hasn't budged, even as other industries with poor gender diversity show improvements.
The issue matters because women venture capitalists have a direct effect on the success of companies other women launch, say female entrepreneurs, including serving as role models and being more likely to spy potential in businesses that target other women.
About 4 percent of senior investing partners in U.S. venture capital are women, according to data from Pitchbook, a consulting firm. That is down slightly from 5 percent in 2010, and about even with 2008.
The numbers compare to 15 percent of executive officers at Fortune 500 companies, 24 percent of tenured faculty at U.S. business schools, and 20 percent of the U.S. Senate - all areas that have shown gains for gender parity in recent years while venture capital has stagnated.
A leading ranking of venture capitalists, the Forbes Midas List, noted just four women on its latest list of top 100 venture investors. A few years ago, five women typically made the list each year. And despite much discussion over the need to improve gender balance over the past few years, many of the top firms still have no senior women partners at all.


text:
In her keynotes, Jody Williams speaks to the importance of activism -- something she has dedicated her life to. She is the founding coordinator of the International Campaign to Ban Landmines (ICBL) and a champion of human and women's rights.
Aside from serving as the founding coordinator, Williams worked as the chief spokesman and strategist of ICBL. In this role, she developed the organization to a network of 1300 NGOs throughout 90s countries. In 1997, at a conference held in Oslo, ICBL achieved its objective of an international treaty banning antipersonnel landmines. Shortly after this remarkable achievement, ICBL and Williams received the Nobel Peace Prize.
Williams is also the chair of the Nobel Women's Intitiative and the Sam and Cele Keeper Professor in Peace and Social Justice in the Graduate College of Social Work at the University of Houston. In her keynotes, Jody Williams draws on this long and fruitful career as an activist.


text:
Here's The Thing, Episode 21: A Brief History Of Firestorm [Video]
Subscribe to ComicsAlliance on
If our weekly Ask Chris column isn't enough of definitive comic book (and pro wrestling) opinions for you, good news: ComicsAlliance is proud to present Here's The Thing, a series of videos where you can join our own extremely opinionated senior writer, Chris Sims, as he dives into comics history to explain why you're wrong and he's right.
This week, a few viewers write in to ask about the history of Firestorm, a relatively obscure DC Comics character who was most prominent in the 1980s. As you may already know, obscure DC characters from the '80s are Chris's entire jam, which sends him on a long explanation of who Firestorm is and how he came to be, from the DC Implosion to Brave and the Bold.
Show Notes:
- Catch up on the original Firestorm stories with DC's paperback collection from a few years back.
- Perhaps Firestorm's most enduring contribution to the DC universe was "Curt Nolland," a stand-in for Smokey and the Bandit-era Burt Reynolds, with whom Killer Frost was briefly infatuated.
- Gerry Conway has a long and incredible career in comics, but non-comics readers may recall him as the writer of the episode of Baywatch Nights that had a mummy in it.
- Seriously.


text:
You can't simply implement a technology platform and hope for the best. You will fail. Watch this video to learn five critical success factors in just 3 minutes.
Come and learn from the leaders.
Making Social Collaboration Work is a one-day masterclass event on October 15th in London, UK. It's designed specifically to give you practical advice and lessons learned, shared directly by a panel of leading case study speakers who have made social collaboration work for their organisations.
Learn from companies that have already driven real business value from social collaboration:
How did education and media multinational Pearson encourage collaborative working across 80 countries, consolidating 130 intranets and reducing the time it took to roll out extensive workplace technology changes? You'll find out here.
Whether your preferred approach is a social intranet, an enterprise social network (ESN), a community platform or something else, the fact is that your ultimate social collaboration success will depend on much more than your technology choice.
Get all the details in the downloadable event brochure.
All practical advice. No arm-waving.
This event is not a technology showcase event. There will be no vendor presentations.
You'll benefit from in-depth peer-to-peer discussion, interaction and real-world advice about how your organisation can guarantee business value from a social collaboration investment. Whether you're just starting out or you're looking for ways to reinvigorate an existing initiative, this is a great opportunity to network with others facing the same challenges as you.
Here are 11 reasons why you should attend.
An event for everyone involved in social collaboration.
It doesn't matter if you're implementing a few social features or a full enterprise social network, this event will help you learn from people who have done it already, and get your questions answered.
Get a head start now! Sign up to access our free library of reports, specially curated in association with the Making Social Collaboration Work event.


text:
Cars > Events > Cruisin' Virginia Beach Car Show
Contact Information and Links
- Shaun Nicely
- 757-425-3111
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- Apr 10, 2009 at 12:21 pm
- Planning a photo shoot at this event. Currently have 10 models scheduled to attend! Look for me and my camera in action!!
About Cruisin' Virginia Beach Car Show
The Cruisin' Virginia Beach Car Show turns the boardwalk into a local version of "Route 66" with cars and trucks from yesterday and today competing for trophies and cash prizes at this one-day event. Hot rods, street machines, classic cars, and trucks line the boardwalk for 25 blocks, where thousands of car enthusiasts and families view over 200 vehicles in a beautiful beach setting.
Whether you bring a classic car to show off or cheer your favorite entry at the popular burnout competition, this revved-up event is guaranteed to crank up both spectators and participants! The controlled burnout competition, always a crowd-pleaser, will be held Saturday at 5:30pm at the municipal parking lot at 18th Street and Arctic Avenue at the oceanfront.
The hours of the car show are 10:00am-4:00pm on the boardwalk, with the burnout competition held from 5:30pm-8:00pm.
Date and Time
Sat Apr 18, 2009 from 10:00 am – 4:00 pm (EDT)
Event Schedule
Times are shown in US/Eastern time.
|Apr 18, 2009
|10:00 am – 4:00 pm
|Boardwalk Show
|Apr 18, 2009
|5:00 pm – 8:00 pm
|Burnout Competition
Location and MapBoardwalk
18th Street
Virginia Beach, Virginia 23451
United States
Event Attendees 1 – 2 of 2
Photo Albums and Videos 1 – 2 of 2
- Crusin Virgina Beach car show
- By ME_Photography
- Photos: 70
- 2009 Cruisin the Beach Boardwalk Show
- By carfanatic1
- Photos: 222


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1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research
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We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.
Date Published: 12/26/2012, Issue 70; doi: 10.3791/4322
Keywords: Immunology, Issue 70, Cellular Biology, Molecular Biology, Infection, Infectious Diseases, Microbiology, alveolar type II epithelial cells, mouse, respiratory tract, lung, cell sorting, flow cytometry, influenza, autoimmunity
Gereke, M., Autengruber, A., Gröbe, L., Jeron, A., Bruder, D., Stegemann-Koniszewski, S. Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies. J. Vis. Exp. (70), e4322, doi:10.3791/4322 (2012).
Throughout the last years, the contribution of alveolar type II epithelial cells (AECII) to various aspects of immune regulation in the lung has been increasingly recognized. AECII have been shown to participate in cytokine production in inflamed airways and to even act as antigen-presenting cells in both infection and T-cell mediated autoimmunity 1-8. Therefore, they are especially interesting also in clinical contexts such as airway hyper-reactivity to foreign and self-antigens as well as infections that directly or indirectly target AECII. However, our understanding of the detailed immunologic functions served by alveolar type II epithelial cells in the healthy lung as well as in inflammation remains fragmentary. Many studies regarding AECII function are performed using mouse or human alveolar epithelial cell lines 9-12. Working with cell lines certainly offers a range of benefits, such as the availability of large numbers of cells for extensive analyses. However, we believe the use of primary murine AECII allows a better understanding of the role of this cell type in complex processes like infection or autoimmune inflammation. Primary murine AECII can be isolated directly from animals suffering from such respiratory conditions, meaning they have been subject to all additional extrinsic factors playing a role in the analyzed setting. As an example, viable AECII can be isolated from mice intranasally infected with influenza A virus, which primarily targets these cells for replication 13. Importantly, through ex vivo infection of AECII isolated from healthy mice, studies of the cellular responses mounted upon infection can be further extended.
Our protocol for the isolation of primary murine AECII is based on enzymatic digestion of the mouse lung followed by labeling of the resulting cell suspension with antibodies specific for CD11c, CD11b, F4/80, CD19, CD45 and CD16/CD32. Granular AECII are then identified as the unlabeled and sideward scatter high (SSChigh) cell population and are separated by fluorescence activated cell sorting 3.
In comparison to alternative methods of isolating primary epithelial cells from mouse lungs, our protocol for flow cytometric isolation of AECII by negative selection yields untouched, highly viable and pure AECII in relatively short time. Additionally, and in contrast to conventional methods of isolation by panning and depletion of lymphocytes via binding of antibody-coupled magnetic beads 14, 15, flow cytometric cell-sorting allows discrimination by means of cell size and granularity. Given that instrumentation for flow cytometric cell sorting is available, the described procedure can be applied at relatively low costs. Next to standard antibodies and enzymes for lung disintegration, no additional reagents such as magnetic beads are required. The isolated cells are suitable for a wide range of functional and molecular studies, which include in vitro culture and T-cell stimulation assays as well as transcriptome, proteome or secretome analyses 3, 4.
Details regarding required reagents and materials are listed in the table at the end of the protocol below. Before starting work, prepare 15 ml tubes (one per mouse) containing 4 ml of dispase and pre-warm them to 37 °C in a water bath In a heating block, shortly heat small aliquots of 1 % low-melt agarose (in water) to 95 °C until liquefied and subsequently cool to 45 °C until use.
1. Preparation of the Mouse Lung
2. Enzymatic Digestion of the Lung Tissue
If desired, a bronchoalveolar lavage fluid sample can be prepared by flushing the lungs through the catheter inserted in the trachea with PBS or medium before the next step.
3. Preparation of the Lung Cell Suspension
4. Antibody Staining for Flow Cytometric Cell-sorting
5. Cell-sorting
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When sorting lung cell suspensions isolated from healthy mice, the AECII gate will typically account for about 42 ± 10 % of all events. This percentage can be noticeably lower when mice with respiratory conditions such as a viral infection are used, as the initial cell suspension will contain a considerably higher proportion of lymphocytes and other immune cells recruited to the airways. For AECII isolated from IAV infected lungs on day 3 following infection we have observed a reduction of the frequency of cells in the AECII gate by roughly 50 %.
A typical sort as well as re-analysis of the sorted cells is depicted in Figures 1b and 1c. As indicated, the purity of the isolated cells accounts roughly 92 ± 5 %. In Figure 2a,we show that successful separation of pure cell populations can be confirmed by staining for surfactant-proteins C, which is expressed exclusively by AECII 16, 17.
We have observed that the number of AECII isolated per single animal strongly depends on the mouse strain used. Whereas BALB/c mice typically yield 1 x 106 AECII/mouse, the C57Bl/6 strain only yields 1 - 3 x 105 AECII/mouse. The number of mice used per experiment therefore depends on the mouse strain as well as the type of subsequent analyses to be performed.
Regarding viability of the retrieved AECII, we typically find viability rates of around 90 % following flow cytometric cell-sorting. This high proportion of viable cells then allows direct functional analyses as well as short-term culture of the isolated primary murine AECII.
Figure 1. Overview over the gating strategy used for flow cytometric isolation of primary murine AECII by negative selection. (A) Schematic overview over the gating strategy. (B) Representative plots of a lung cell suspension sort. (C) Representative result of a re-analysis of the sorted cells. Click here to view larger figure.
Figure 2. Characterization and molecular analysis of sorted primary murine AECII. (A) Cytospins of sorted AECII were stained for the surfactant protein C (SPC). Compared to phase contrast microscopy, nearly 100 % of cells were found to be SPC positive. (B) Representative RNA profile of sorted primary murine AECII after RNA-isolation. Specific peaks for 18S and 28S ribosomal RNA together with the calculated RIN factor represent RNA integrity and quality for intact isolated RNA from AECII.
Figure 3. Analysis of the influenza A virus nucleoprotein (NP) expression in primary murine AECII isolated from infected mice. C57Bl/6 mice were intranasally administered phosphate buffered saline or influenza A virus PR8/A/34 on days 1, 2 or 3 prior to AECII isolation. (A) Representative plots of lung cell suspensions from an uninfected as well as a three-day infected C57Bl/6 mouse stained for sorting. (B) Influenza A virus NP expression in sorted AECII was analyzed through intracellular staining with an NP-specific antibody and subsequent flow-cytometric analysis. (C) The table shows the mean fluorescence intensity (MFI) of the NP staining.
Figure 4. Analysis of the influenza A virus nucleoprotein (NP) expression in ex vivo infected primary murine AECII. (A) Representative flow-cytometric analysis of ex vivo influenza A virus PR8/A/34 infected AECII by intracellular staining for the influenza A virus NP 6 hr post infection. (B) Summary of representative results from NP-staining of ex vivo influenza A virus infected AECII 6 h post infection with different virus dilutions.
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Our protocol for the isolation of murine AECII by flow cytometry offers a rapid way of accessing primary cells from the mouse lung for a whole range of functional and molecular studies. The described procedure yields highly viable and pure populations of AECII that are sufficient in number for direct subsequent analyses, such as RNA isolation (see Figure 2b) and transcriptome studies. For functional applications, it is also possible to culture the isolated cells, allowing e.g. the generation of AECII conditioned medium or co-culture experiments. As a benefit especially for these functional studies, the isolation of primary cells by negative selection as described here yields untouched cells which have not been subject to antibody binding. However, despite the advantages of studies in primary AECII over those performed in cell-lines, there are practical limitations to the use of these primary cells. Next to the mere limitation in numbers, which might not meet the requirements of studies requiring extensive screening, primary AECII survive in culture only for a restricted time which we have observed to average around 48 hr. In these short-term culture experiments, we have based the choice of culture medium on the nature of the subsequent assays the AECII conditioned medium was used in and have achieved satisfactory results with IMDM (IMDM Glutamax, Gibco Cat. No. 31980) supplemented with 10 % FCS, standard antibiotics and 0.25 mM β-mercaptoethanol.
The protocol described here displays a relatively easy and rapid way of isolating viable and pure primary murine AECII in good numbers. The purity of the yielded cells after separation critically depends on the procedure of cell-sorting. A clear and strong staining of the cell populations which are to be excluded is equally important as stringent gating on the SSChigh events, so that contaminations with lymphoid cells as well type I epithelial cells are minimized. Regarding the yield of separated cells, we have observed that this is maximized by extensive flushing of tubes, filter meshes and plates during preparation of the crude cell suspension as well as by complete disintegration of the tissue following enzymatic digestion. One main benefit of working with primary AECII is that they can be isolated directly from hosts suffering from diseases of the respiratory tract. Such AECII will have been exposed to all factors present in their natural micro-environment and therefore well reflect the in vivo situation. Unlike for human AECII 18, 19, this can be extended to a wide range of experimental systems when working in murine models. We have been taking advantage of this in studies regarding autoimmune inflammation as well as viral infection of the lung. Thereby we found that AECII from mice suffering from CD4+ T cell mediated respiratory inflammation express a wide range of inflammatory genes which demonstrates their potential for regulating lung immune responses 3. Furthermore, we could show that AECII display self-antigens through major histocompatibility complex class-II molecules which results in the functional activation of auto reactive CD4+ T cells. At the same time AECII maintain lung tolerance by secreting factors promoting the induction of Foxp3+ regulatory T cells 4.
Regarding viral infection of the lung, we have successfully isolated primary AECII from mice infected with influenza A virus. As mentioned above, here AECII account for a smaller proportion of the whole lung cell suspension, as considerable numbers of immune cells are recruited to the lungs in response to infection (see Figure 3a). Intracellular staining of the influenza A virus nucleoprotein (NP) within the isolated cells shows increasing expression of the viral protein over the course of time as shown in Figures 3b and 3c. These AECII, triggered by the virus in vivo, display a valuable tool for studies regarding the molecular and functional phenotype of the airway epithelium during respiratory viral infection as well as during recovery. However, as AECII are the primary target of influenza A virus and there is substantial destruction of the epithelial lining, the rate of cells yielded from infected mice decreases over the course and with severity of the infection. Also, as the virus repeatedly replicates and infects new cells, the isolated AECII have not been subject to infection at a single defined point in time. Thus, these studies are ideally complemented by experiments using ex vivo infected primary murine AECII from healthy mice where infection is better synchronized and which, as assessed by intracellular viral NP staining shown in Figures 4a and 4b, can yield higher rates of infection depending on the viral dose used.
Taken together, flow cytometric negative selection as described here displays a rapid way of isolating pure and viable primary murine AECII. These cells are suitable for a wide range of analyses and are a valuable tool for extending our knowledge of the role of AECII in immune regulation in the respiratory tract in vivo.
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No conflicts of interest declared.
We would like to thank M. Höxter for technical assistance in sorting primary murine AECII from biosafety level 2 samples.
This work was supported by grants from the German Research Foundation (DFG) to DB (SFB587, TP B12 and BR2221/1-1) and a stipend from the Hannover Biomedical Research School (DFG GSC 108) to AA. DB is supported by the President´s Initiative and Networking Fund of the Helmholtz Association of German Research Centers (HGF) under contract number W2/W3-029.
|Catalog Number
|indwelling cannula Introcan 22G
|Dispase, 100 ml(5000 caseinolytic units)
|BD Biosciences
|aliquot to 4 ml in 15 ml tubes, store at -20 °C
|Biozym Plaque Agarose
|1% w/v in H2O
|Deoxyribonuclease I from bovine pancreas, 2000 Kunitz units/vial
|freshly dissolve content of 1 vial in 300 μl DMEM
|used as provided by manufacturer (Low Glucose, Pyruvate, HEPES)
|cell strainers (100 μm, 75 μm)
|BD Falcon
|352360, 352350
|nylon mesh(48 μm, 30 μm)
|Bückmann GmbH
|03-48/26-1020, 03-30/18-108
|CellTrics 50 μm filter
|anti-mouse CD16/CD32
|clone 93; purified
|anti-mouse F4/80
|clone BM8; APC coupled
|anti-mouse CD11b
|clone M1/70; PE coupled
|anti-mouse CD11c
|clone N418; APC coupled
|anti-mouse CD45
|clone 30-F11; purified
|anti-mouse CD19
|eBio 1D3; PE coupled
|polyclonal goat anti-rat IgG
|BD Pharmingen
|polyclonal, PE coupled
Antibodies coupled to alternative fluorochromes can be used, depending on the flow cytometer and lasers available.
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Duct Insulation
1 Attachment(s)
First post! Huge fan of the site, and finally decided to register and join in on the fun. I am a first time home-buyer and budding DIY'er in search of direction and advice.
Not sure the cheesy intro was entirely necessary, but it seemed proper as I've been silently stalking the forums for over a year now. :)
Anyway, to the point. I have a 1900's ranch style home. On the large scale, my plan is to make the home more energy efficient. Insulating walls, attic space, etc. I am starting with the unconditioned crawl space.
I believe I have a fairly simple heating system, my trunk line runs the length of the house, with all branch lines running to appropriate room vents. With the exception of my return lines, all the duct work is in the crawl space and I feel I am losing efficiency to leaks, and lack of insulation.
As of right now all branch lines are 5-6" metal (unknown exact). My plan is to seal any leaks, and joints first. I have identified a major leakage issue on many of the lines where they make the connection to the trunk. I have enclosed an image of this. Please reference the shoddy duct tape job someone deemed acceptable.
1. What is the recommended method to sealing these areas and leaks? Should I keep the connections as is, or is there some type of fitting or collar that would be better to connect to the trunk.
I spoke with a gentleman at a local plumbing store. He advised I should replace all the branch lines with pre-insulated flex. I have mixed feelings about this as I know if not installed correctly, the flex ducting can create an air loss due to friction. This and the the system can be more susceptible to damage rodents and animals. Branch lines seem fine, and it seems it would be easier to insulate them rather than replace the lines.
2. What is recommended practice for this? Home depot carries a fiber glass insulation for this, but is only rated r-6. Is there a fiberglass roll in r-8? Where can I find this? Are the foil insulation rolls claiming r-8 worth a damn?
Thanks very much for any ideas/suggestions!
Someone used duct tape which is wrong, it should have been foil tape instead.
For the rest it may be best to use mastic.
It also looks like someone tryed to do a DIY job on there it connects to the trunk line.
is the branch line hanging on the screws? i.e. all the weight is pulling on the screws
I don't believe it is "hanging" by any means. There are screws randomly placed around the whole circumference of the connection. The screws with the alternating in-out tabs seem to be holding it in place okay. I think the biggest concern is just the seal. This connection is one of the better ones under there. There are more obvious leakage issues, and even worse duct tape patch jobs on a good amount of them.
Ironic the one thing duct tape can't be used for is ducts. I would use the mastic on the larger gaps and then foil tape on all seams even if they don't seem to leaking now. And remove all existing duct tape. I am not an insulation guy and you don't say where you are located, but would the difference between R6 and R8 be that much.
I am in Colorado Springs, CO. South of Denver.
I'm not really sure if I NEED the r-8 over the r-6...will the difference be pretty nominal??
As far as mastic goes, does it go on with a brush?
Brush works good. I have been sealing all my duct work and have an old tooth brush I am using to get at the tight spots. The foil and bubble duct wrap is a good choice for the same reason, you can wiggle it into tight spots. My concern is more for condensation during AC operation than heat loss.
No AC right now, so no worries there. I did find this on Amazon:
Anyone have any experience with such a product?
Looks like a good product and will do what you want but I really don't know if that's a good price or not. Between that and sealing your air leaks you should really notice a difference.
Foil tape or mastic are great. Mastic is probably best since its a crawlspace so you probably don;t care if it looks sloppy.... Then treat the crawlspace as a whole (seal and insulate properly). There is a lot of good info on this over in the Insulation section.
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text: Once I left the safety of the walls surrounding my encampment, I came upon a peasant who was in need of help. You can choose to help him, or go about your merry way - I chose to help and when I got down off of my horse to fight some nearby bandits I was wishing I had just stayed the course and ignored the guy. The bandits themselves were not so hard to defeat, even with my character being a total weakling at this point, but wolves ganged up on me while I was fighting said bandits and killed me; thus ending my demo. So to be honest, I have no idea what the actual duration of the demo is, but I do know that if you die it is over.


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In patent disputes, obviously, there are winners and losers.
For those keeping count in Germany, score another win for Apple. On Jan. 31st, the Higher Regional Court in Dusseldorf ruled that the Samsung Galaxy Tab 10.1 violates a German anti-competition statute. Previously, the ruling was based on patent infringement but in response to Samsung's latest appeal on anti-competitive factors, the ruling was revised with a similar result.
Additionally, the court ruled that the Samsung Galaxy Tab 8.9 also violates the German law.
But all is not lost in Germany for the electronics giant. The Galaxy Tab 10.1N is still being sold, as Samsung's modifications were acceptable to Apple and the courts.


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2006 Topps 1952 Edition Rookie Baseball Hobby Box
- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Sports
- Source: eBay
- Sold Date: Jan 19, 2007
- Channel: Auction House
You are bidding on a factory sealed box of2006 Topps 1952 Edition Rookie Baseball Hobby Box
PRODUCT INFO:1952 Edition presents all of this year's brightest rookies on the memorable backdrop of the Topps 1952 card design - a classic look that Topps created over 50 years ago! Printed on grey-back stock and showcasing Topps classic photography, 2006 Topps Rookies - 1952 Edition offers every 2006 "Rookie Card" eligible player, as well as Autographs at a rate of 3 per box and NEW Ticket to Stardom Relics. This is a "can't miss" collecting experience for all Topps and Rookie collectors!
3 AUTOGRAPHED CARDS PER BOX! Ships immediately!!!!! Insurance optional. Not responsible for uninsured items. Low starting bid and NO RESERVE.
All sales final. Good luck bidding.


text:
There are many types of dads around the golf course, not one is the same.
Many who've played golf at any level may have a fond memory, a moment or a life lesson they've shared with their father along the way, on or off the course.
Canadian professionals Lorie Kane and Adam Hadwin both have careers that were jump started by dads who were teaching professionals, while others like Justin O'Leary, associate teaching professional at the National Golf Club of Canada, had a father that simply wanted his kids to enjoy golf.
"Dad told me that I was blessed with God's gift to play golf," said Kane of her father Jack. "He read [Ben] Hogan's book Five Lessons and that's what he taught [my sister and I].
"Even to this day, he still goes back to those if he sees something that I should be doing or not doing, just to remind me."
Hadwin, who finished in a tie for fourth at the 2011 RBC Canadian Open, also had the fortune of having a dad well versed in the game. His father, Gerry, taught him the foundation of his swing from an early age.
"When he was trying to help me out he obviously had all the right stuff, he had all the right things to say," said Hadwin. "Just as a teenager you didn't want to listen to your parents, so we butted heads a lot."
Hadwin's story isn't unlike many others, sometimes as a teenager listening isn't easy. However, with age comes the realization that dad was probably right and that generally fathers are in their child's court.
"He's always believed in me, he's always been behind me," said Hadwin. "He's like quiet and proud."
Kane, like Hadwin, also had a few moments that weren't easy with a father so involved in a game. She still seeks advice from him but what's different now is that she initiates the conversation and it's from dad not 'golf dad'.
"I actually fired him a long time ago because it was easier for me, I wanted to have my dad instead of my coach," said the four-time LPGA winner. "Trust me it was a difficult thing to do but at the same time it probably saved a father-daughter relationship, I don't have a bigger fan than my mom and dad, they're there if I need them."
Luckily, for Kane, her father understood and the tough decision turned into a positive situation. She's still thankful today for his involvement throughout her career.
"I obviously wouldn't be in golf if it wasn't for dad," she said.
The more common dad may be the one that just enjoys the game and knows little about the golf swing. They're the kind that drives their children to the course when the sun is rising and picks them up when the sun is setting.
Justin O'Leary's father, Kevin, was one of those. He gave a selfless gift to his family for the love of the game.
"My older brother joined, then I joined [Westfield Golf and Country Club in NB], then my younger brother did and finally my sister," he said. "We weren't a rich family, my dad backed out because we couldn't afford it. He let us play. He was always our no. 1 fan."
O'Leary's story is a touching one, as his father passed away April 7, 2009. It makes one wonder if the 'Golf Gods' really do exist.
In that year, O'Leary was named Ontario PGA Associate Pro of the Year alongside his friend Sean Casey, director of instruction at Glen Abbey, who was named Canadian PGA Teacher of the year.
Both grew up playing golf together in New Brunswick, and their hometown newspaper wanted to do a story on their recent success in the game. The story was set to print the eve of Tiger's incident at Isleworth and never saw the papers, something that frustrated O'Leary who was looking forward to it.
Almost six months later, on April 7, 2010, a day he was dreading as it was the one-year anniversary of his father's death, the story of their teaching success that had been set to print months earlier appeared on the main page of the newspaper. He had no idea it was even being printed.
"It was just too surreal, on the anniversary, it's the front-page story, it was crazy ironic," he said. "All the time I pushed that article aside, it was the best thing ever when mom opened that paper that morning."
Without that membership at the club next door, O'Leary may have never found the opportunity to break into an industry that has become a successful career for him. He and his family have never forgotten what their father did for them.
Morgan Bell has played golf since the age of 5 and credits her father John for starting her in the game after taking her for many late-evening nine-hole rounds at Stanhope Golf and Country Club on PEI in their three-wheel golf cart.Report Typo/Error


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The Doors' Keyboard Counterpoint Goes Silent: Remembering Ray Manzarek
By Mark Goldberg On May 21, 2013 · Leave a Comment · In Music, National Public Radio
Ray Manzarek, the founding keyboardist of the Los Angeles rock band The Doors, died in a clinic in Germany on Monday after a lengthy battle with cancer, according to his publicist. He was 74.
One of the biggest-selling bands of all time — and one of the most controversial — The Doors came together in 1965, after Manzarek moved to Los Angeles and recruited Jim Morrison to sing with his college band. Manzarek grew up on Chicago's south side and resisted piano lessons when he was young, until he heard Chicago blues and jazz on the radio. His keyboard playing on such Doors classics as "Light My Fire," "Riders on the Storm," "Break on Through (to the Other Side)" and more provided a blues and jazz counterpoint to Morrison's poetic swagger.
After Morrison died in 1971, The Doors recorded two albums with Manzarek singing some of the lead vocals, but the band eventually broke up. Manzarek recorded several solo albums, collaborated with poets, and produced for other bands, including X, another trailblazing Los Angeles group. In 1998, Putnam published Manzarek's autobiography to critical acclaim, though Light My Fire: My Life with the Doors, was more about Morrison than Manzarek. He also wrote two novels. In 2002, Manzarek reunited with The Doors' guitarist Robby Krieger to play the band's songs live.
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Travon Woodall Bio(pdf) | Woodall Photo Gallery | Career Statistics | 2009-10 Game-by-Game | 2008-09 Game-by-Game |
A true point guard, Travon Woodall (pronounced Tray-von) played a key role off the bench last season. A leader on offense, Woodall improved with each and every game last season as he led the Panthers in assist:turnover ratio and ranked second on the team in assists. The quick and multi-talented Woodall is a great passer and scorer who causes matchup problems. He is an experienced big-game player who guards, scores and has the ability to emerge as a natural floor leader. Woodall is quick with the ball, athletic and is an effective penetrator. He received a medical redshirt for his first season at Pitt and with a full 2009-10 season under his belt, Woodall will have the opportunity to have a high impact year for Pitt in 2010-11.
Played in all 34 games and started 11 games at point guard...Ranked second on team in assists with 108 (3.2 apg)...Finished with a team-leading 1.86 assist:turnover ratio, a mark that ranked him No. 118 nationally in that category...Over the season's last 10 games, compiled a 3.22 assist:turnover ratio (29 assists/9 turnovers)...Reached double figure scoring in five games on year with one double figure assist game...Earned Most Improved Player Award at team banquet...Dished out five assists, grabbed five rebounds and scored four points in 17 minutes of NCAA Tournament Second Round game vs. Xavier, 3/21...Scored 10 points (4-7 FG), hit 2-3 3-pointers in 24 minutes vs. Oakland, 3/19...Scored six points (all six in the first half) and dished out four assists at St. John's, 2/27...Scored eight points (4-8 FG) at Notre Dame, 2/24...Played a key role off the bench with 12 points, six assists and a career-high seven rebounds in 31 minutes vs. West Virginia, 2/12...Registered six assists vs. DePaul, 12/28...Finished with 10 points vs. Kent State, 12/29...Scored a career-high 19 points and set or tied career-highs for free throws (7), free throws made (10), field goals made (10), 3-point field goals made (2) and rebounds (6) vs. Wichita State, 11/23...Dished out seven assists in a career-high 33 minutes vs. Eastern Kentucky, 11/19...Notched first career double-double with career-highs of 10 points (4-10 FG) and 13 assists vs. Binghamton, 11/17. The 13 assists ranked tied for the seventh highest game total in school history...Made first career start with eight points vs. Wofford, 11/13.
Earned a medical redshirt for the 2008-09 season after suffering a severe knee injury...Played in the season's first 10 games off the bench...Grabbed a career-high five rebounds vs. Miami, Ohio, 11/17...Played 15 minutes, totaled five assists and hit first 3-point field goal in career debut vs. Fairleigh Dickinson, 11/14.
A 2008 graduate of St. Anthony High School in Jersey City, N.J. where he lettered four seasons for head coach Bob Hurley...Guided St. Anthony to a perfect 32-0 record, No. 1 national ranking and national championship by USA Today...Led the Friars to the New Jersey Non-Public B state title as a senior...Second Team Associated Press All-State selection after averaging 10.0 points and 3.0 assists per game as a senior...Helped St. Anthony defeat Newark Science Park 69-36 in the Tournament of Champions title game...Registered 16 points and seven rebounds in leading the Friars to a 76-41 win over Immaculata in the Tournament of Champions semifinal...Earned Most Valuable Player honors after scoring 22 points and guiding Team USA to a 120-115 win over Team Florida at the Senior Showcase...As a junior, guided St. Anthony to a 28-1 record and appearance in the New Jersey state Non-Public Class B state final before losing to No. 2 nationally ranked St. Patrick...St. Anthony finished ranked No. 7 by USA Today...Averaged 10 points and three assists per game as a junior...Three-time Newark Star-Ledger All-Area team selection...Averaged 10 points and 3.0 assists while helping guide St. Anthony's to the New Jersey North Public B final as a sophomore...Selected as a "Fabulous Freshman" and competed in the MetroHoops North-South All-Star game...Played at the AAU JamFest in Pittsburgh, the first time he stepped foot on campus in 2007...Played in the Eddie Griffin Challenge (New Jersey vs. Philadelphia area All-Star preps)...Earned All-Star honors at the prestigious ABCD Camp in Hackensack, N.J. and 5-Star Camp following his sophomore season...Played on the New Jersey Playaz AAU team coached by Jimmy Salmon...Invited to compete at "Camp Next" as a freshman...Competed in the Jordan Brand Classic All-Star Game in April, 2008.
Born Travon Woodall on April 14, 1989...Son of Theresa Ratliff...Has one sister Shataya Ratliff...Lives with his godfather and godmother Jorge and Samar Fontan...Godbrother is Jiovanni Fontan, who plays basketball at Fordham...Began playing basketball while living in Brooklyn, N.Y....Introduced to basketball by his cousin Khalief Woodall, who suffered a tragic death at age 11...Teammates call him a "born leader"...Played a starring role in the documentary The Street Stops Here, a movie about Bob Hurley and the St. Anthony basketball program...Has known fellow Pitt players Ashton Gibbs and Nasir Robinson since the seventh grade...Played on an AAU team with Gibbs...One of six players from his high school senior class to sign with NCAA Division I schools: Mike Rosario (Rutgers, now at Florida), Tyshawn Taylor (Kansas), Jiovanni Fontan (Fordham), Alberto Estwick (Fordham) and A.J. Rogers (Saint Joseph's)...Selected Pitt for its graduation rate, environment and coaching staff...Also selected Pitt because of the lineage of point guards and opportunity to learn from Levance Fields and current assistant coach Brandin Knight...Communications major.
Points: 19, Wichita State, 11/23/09.
FG made: 5, Wichita State, 11/23/09.
FG attempts: 10, four times- last vs. Texas, 11/24/09.
3FG Made: 2, six times- last vs. Oakland, 3/19/10.
3FG attempts: 6, Wofford, 11/13/09.
FT made: 7, Wichita State, 11/23/09.
FT attempts: 10, Wichita State, 11/23/09.
Rebounds: 7, West Virginia, 2/12/10.
Off. Rebs.: 2, vs. Duquesne, 12/2/09.
Def. Rebs.: 6, three times- West Virginia, 2/12/10; Wichita State, 11/23/09; Eastern Kentucky, 11/19/09.
Assists: 13, Binghamton, 11/17/09.
Steals: 4, two times- Wichita State, 11/23/09; Indiana, Pa., 11/22/08.
Blocks: 2, Eastern Kentucky, 11/19/09.
Minutes: 34, vs. Texas, 11/24/09.
Points: 19, Wichita State, 11/23.
Rebounds: 7, West Virginia, 12/2.
Assists: 13, Binghamton, 11/17.
Steals: 4, Wichita State, 11/23.
Blocks: 2, Eastern Kentucky, 11/19.
Minutes: 34, vs. Texas, 11/24.
Woodall returns to make his point, Feb. 16, 2010, Ray Fittipaldo, Pittsburgh Post-Gazette
Freshman guard puts together his coming of age game, Feb. 14, 2010, Kevin Gorman, Pittsburgh Tribune-Review
Woodall gets to the point in Backyard Classic, Feb. 13, 2010, Ray Mernagh, NBE Basketball
Brooklyn Guard Sets Pace for Pitt, January 16, 2010, Tim Sullivan, New York Post
Woodall Ready for Point at Pitt, Nov. 12, 2009, Adam Zagoria, SportsNetNY
Focus on Travon Woodall, Oct. 30, 2009, Mike Gladysz, Panther Eyes
Boys hit the Big Time (Travon Woodall and Mike Rosario), Dec. 30, 2008, Adam Zagoria, SportsnetNY
Brooklyn Guard Making "Rough" Transition at Pitt, Dec. 6, 2008, Tim Sullivan, New York Post
Pitt Duo Returning Home to New Jersey, Nov. 27, 2008, Adam Zagoria, SportsNetNY
Freshmen Guards Pick up Slack for Pitt, Nov. 3, 2008, John Grupp, Pittsburgh Tribune-Review
With Fields hurt, Pitt freshman Travon Woodall Steps in at Point, Oct. 17, 2008, Pittsburgh Post-Gazette
Pitt Lands Second Recruit in Three Days, Jan. 19, 2007, John Grupp, Pittsburgh Tribune-Review
RS Sophomore
Brooklyn, N.Y.-Paterson, N.J.
High School:
St. Anthony H.S. (N.J.)
Height / Weight:
5-11 / 190
George Signs Financial Aid Agreement with Pitt
Artis & Young Ink NBA Summer League Deals
Pitt Adds Combo Guard Stewart to 2017 Recruiting Class
Pitt Announces Basketball Staff Changes
Pitt Men's Basketball Season Tickets Now on Sale


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Watered-Down Term Limits Bill Up for Approval Today in Texas House
The Texas House today takes up the first tem limits bill ever to be given an even chance of passing the Legislature.
"All state wide Executive Branch officials will be limited to a total of eight years in their office," George Seay, a Dallas hedge fund manager who is the head of Texans for Term Limits, told 1200 WOAI news.
The measure would not limit the terms of the members of the Legislature who will vote on the bill, nor would it limit the terms of judges, but Seay says this is a 'start' toward making that happen.
"If we establish this beachhead, it will be very hard for the Legislative and Judicial branches not to have term limits, but the Executive branch should."
He says the bill currently on the table would term limit statewide Executive offices, and statewide elective offices like Texas Railroad Commissioner and State Board of Education members.
The term limit bill has already passed the State Senate. As a constitutional amendment, it requires a two thirds vote of each house to be approved, and then the proposal would be voted on by the people in November.
Governor Perry, who has served in that office since 2000, making him the longest serving statewide office holder in Texas history, would not be able to veto the legislature.
Seay says the term limits law would not be retroactive, meaning state lawmakers like former House Speaker Tom Craddick, who was first elected to the Texas House, amazingly, in 1968, could still continue to serve if the term limits law were extended to the House. Seay says that is only fair.
Seay says if this term limits law is approved by the House and by the people, his group will be right back in the Legislature in 2015, seeking to approve a measure that expands term limits to additional offices.
He says his ultimate goal is a law that limits the total number of time that any one individual could serve in all elective offices combined, ending the process of skipping from city or county office, to the State House, then the State Senate, and so on.
"We need term limits across all three branches of government," he says. "And we need them so there will be a maximum time of service for a politician."
Ironically, the main sponsor of the term limits, Sen. Kevin Eltife (R-Tyler) was elected to the Legislature because term limits in his home town prevented him from running for re-election as Mayor of Tyler.


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Oxfam Unwrapped Animal Magnets
Wanna give a little bit extra?
Buy one of these fantastic fridge magnets and support workers from Alura Amara, a fair trade producer in Indonesia. Alura Amara was founded as an alternative for young Javanese who were forced to migrate to the big cities in search of work, providing work in their hometown instead. The organisation provides excellent working conditions, daily education programs and a rice subsidy.
Magnets are available to buy with any printed or blank Oxfam Unwrapped gift card purchase*. We'll remind you of the magnets later when you're buying your Unwrapped cards.
Choose from a goat, pig, chicken or duck!
* Magnets are not a donation to Oxfam Australia and are not tax-deductible. Price of magnets is GST inclusive. Magnets not available with e-cards.


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Hello: here is our suggestion:
1 – Piano Music Pack, wich contains five piano tracks, suitable for the piano part.
2 – Lost Tears, for the melanchonic approach
3 – The Shadow of the Beast; another warm tune.
4 – Angle of Paradise, for hope and bright moments
5 – Children's Morning smile, if you need to put some motivation in your ending.
If you like something, but need edit or customization, please, contact us : email@example.com. We'll do the change for free.
Best regads and good luck for your projects!
I believe, this one song is made for this kind of project. With it's calm intro and thw growing style it provides a feeling of hope at the end. That's why it's called "Joy Theme Movie Trailer": http://audiojungle.net/item/joy-theme-movie-trailer/470282 Regards berlinsound
Perhaps these tracks would work? Thank you for sharing and good luck.Moments of Peace
Follow The Dream http://audiojungle.net/item/follow-the-dream/2367836?sso
Hi! You may consider this onehttp://audiojungle.net/item/inspirational/2903985
Not sad, not really happy but throws a hint of hope
Hi, probably these tracks gives you that mood:
check out all my portfolio if you want
look my "Motivation" portfolio )http://audiojungle.net/collections/2327606-corporate-motivation
Hi! Maybe this one: http://audiojungle.net/item/sad-story/234131


text:
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This Item is Discontinued, however these items are available:
Nitecore SmartRing Tactical SRT 6 Night Officer Flashlight - CREE XM-L2 White LED - Red, Green and Blue LEDs - 930 Lumens - Uses 1 x 18650 or 2 x CR123As - Grey
(7 Reviews)
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Nitecore SmartRing Tactical SRT 7 Revenger Flashlight - CREE XM-L2 T6 White LED - Red, Green and Blue LEDs - 960 Lumens - Uses 1 x 18650, 2 x 16340s or 2 x CR123As
(17 Reviews)
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Nitecore SmartRing Tactical SRT 3 Defender Flashlight - CREE XM-L2 T6 White LED - Red, Green and Blue LEDs - 550 Lumens - Uses 1 x 14500, 1 x AA, 1 x CR123A or 1 x RCR123A
- Item #: NITECORE-SRT3-XML2-BLACK
- Brand: Nitecore
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Compact and TacticalThe Nitecore SRT3 Defender is an easy-to-use LED flashlight designed to be carried every day. The tactical configuration and variety of colored LED's means this light is ideal for everyone from casual users to police and military personnel in need of a back-up flashlight. The SRT3 fits easily into your pocket and can provide huge outputs in an instant. Advanced LED technology adds efficiency and longevity to the truly unique design of the Defender.
Advanced LED TechnologyNitecore started with a compact body that measures less than four inches in length, then packed a powerful XM-L2 T6 LED inside. In its highest mode, the SRT3 is capable of producing 550 lumens! The machined aluminum body is rugged enough for tough, outdoor use and features a military-spec anodizing to protect against scratches and corrosion. Precision construction and O-ring seals allow the Defender to remain resistant to water at up to two meters of submersion. The Defender's unique configuration allows it to be run off of a single CR123A battery, or one AA battery when using the included AA extender.
Revolutionary ControlThe SRT3's interface is a revolutionary combination of a tail-switch interface and a Smart Selector Ring at the top of the light. The tail-switch allows easy one-handed operation, even in conjunction with a firearm or other weapon. When the switch is activated, the Defender jumps into action at whatever setting its rotator ring has been set to. To adjust the output, simply twist the selector ring and watch as the lumens grow and fade, allowing you to stop at the exact brightness level you need. Keep twisting to the right, and the light will click into its 550 lumen turbo mode, and then into its blinding strobe.
Red, White, And Blue LED'sAs if all of this wasn't enough, Nitecore also included a red/blue auxiliary LED for help preserving night vision and tracking game. The red and blue settings, as well as an ultra-low mode and an SOS beacon can be accessed by twisting the rotator ring to the left, with each mode located with another click. The SRT3 Defender is a member of Nitecore's larger line of SRT flashlights. The SRT series is defined by their Smart Selector Ring, tactical tail-switch interface, and aggressive profiles. The SRT3 is available with the black finish shown in this listing as well as a grey finish available Here.
- Utilizes the latest CREE XM-L2 T6 LED for a maximum output of up to 550 lumens
- Second generation of 'Crystal Coating Technology' along with 'Precision Digital Optics Technology' provide extreme reflector performance
- Equipped with unique multi-colored (red and blue) tactical/signaling lights
- High efficiency circuit board provides up to 200 hours runtime on lowest output level
- Infinite brightness adjustment from 0.1 to 550 lumens
- 8 modes to choose from (Infinite Brightness 0-550 lumens, Strobe, Turbo, red light, blue light, flashing blue/red, and SOS beacon)
- Reverse polarity protection
- Tail stand capability
- Stainless steel titanium-plated clip
- Stainless steel retaining ring protects core components from damage
- Toughened ultra-clear mineral glass with anti-reflective coating
- Constructed from aero grade aluminum alloy
- Waterproof in accordance with IPX-8 standards
- Includes: holster, removable clip, AA extension tube, lanyard, spare tail cap button, and spare O-ring
- Also available in Grey
- Runs on: 1x RCR123 / 1x CR123 / 1x AA (Batteries NOT included)
- Output mode/Runtime:
RCR123 Battery
- High: 550lm/ .75h
- Low: 0.1/ 150h CR123 Battery
- High: 330lm/ 1.25h
- Low: 0.1/ 200h AA Battery
- High: 180lm/ 1h
- Low: 0.1/ 200h
- Peak Beam Intensity: 4500 cd
- Dimensions:
- Length:
- Without Extender: 3.93" (100 mm)
- With Extender: 4.6" (117mm)
- Head Diameter: 1" 25.4(mm)
- Body Diameter: 1" 25.4(mm)
- Length:
- Weight: 2.57.oz 73(g) (excluding batteries)
|Runtime (Hours):
|Output Modes:
|Variable Brightness, Blue, Red, Beacon, SOS, Strobe
|Compatible Batteries:
|1 x 14500, 1 x 16340, 1 x AA, 1 x CR123A
|Batteries Included:
|Included Accessories:
|Extender Tube, Holster, Lanyard, Pocket Clip, Spare O-ring(s), Spare Tailcap Boot
|Flashlight Usage:
|Every Day Carry, Tactical
|Throw Distance (Max):
|Switch Location:
|Variable Ring
|Product Certifications:
|Battery Junction 30-Day Warranty, Nitecore 5 Year Warranty
|Return Policy:
|Battery Junction Return Policy
|Primary Material:
|Anodized Aluminum
|Product Weight:
|Product Dimensions:
|3.93" (L) x 1.25" (Diameter)1.25" (Head Diameter)1.25" (Body Diameter)
|Country of Origin:
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Nitecore SmartRing Tactical SRT 3 Defender Flashlight - CREE XM-L2 T6 White LED - Red, Green and Blue LEDs - 550 Lumens - Uses 1 x 14500, 1 x AA, 1 x CR123A or 1 x RCR123A
Quitman, TX
5 Stars
I would recommend this item to a friend.
Great EDC
February 17, 2015
I got this 9/14 and carried it every day since then; you can hardly tell it's there. The twist function is tight, smooth, with no gritty fill at all. The blue light is usable, as in you can see the ground a few feet in front of you. The red light would only be good for seeing something at arms length, the ground is barely visible at your feet. The red and blue flashing part has no practical use that I see. The twist control let's you choose just the amount of light you want. Battery life is good and the various battery options is a big plus to me. I use an RCR123 most of the time, but having the option of using a AA is great. This light has an amazing amount of light for being as small as it is. No light will do it all but this one comes close with about 100yds of usable throw and very good spread in turbo or twist down to low to use as a nightlight.
Pros:Battery options Your choice of light out put
Cons:Not really a con but I would of rather had a brighter red light than the flashing red/blue light
Kaneohe, HI
5 Stars
I would recommend this item to a friend.
Great EDC or back up light.
January 21, 2015
I really like this light as it is compact, well built, and weather resistant. I hear from my friends and see some of the reviews that this light is a bit on the pricey side. But I believe that you get what you pay for as this little light again is quite tough. I use it as my back up light when I am in the field and it has never let me down. I have even dropped it on a jobsite, left it overnight in the rain, and found it the next afternoon half buried in mud and the with just a simple rinse this SRT3 was just fine.
Gregg G
San Antonio, Tx
5 Stars
I would recommend this item to a friend.
Impressive, but it has a few oddities.
December 12, 2014
The light output is amazing. Definitely a good pocket flashlight. Like how easy it is to change the light settings. The embedded red and blue LED is a nice twist. The odd part is the "police light" function. It has a red and blue wig wag effect. Its not very bright in that sense, so I don't know what good it would do. My only issue with the light is the tail cap button. For momentary On, you must lightly depress it for about a second or 2 before it comes on. No biggie, just if I press the tail cap, it should come on with out hesitation.
Pros:Very bright. Easily adjustable light settings.
Cons:Momentary On takes a second or two to fire up. Odd "police light" function.
San Antonio, TX
4 Stars
I would recommend this item to a friend.
Great Versatile Light
November 25, 2014
I've owned this light for about a month and I really like it. It's the first one I've owned with a selector ring; the ring was easy enough to memorize & use. I bought this light because of its ability to use AA batteries in a pinch and for the red & blue lights (I figured my kids would think it was cool). The red & blue lights turned out to be more than a gimmick - I use them when walking through a parking lot with my young children at night. I will probably buy a few of these for Christmas gifts.
Pros:Tail on/off switch Will stand up on end Size Weight Multiple light settings Accepts AA batteries if needed
Cons:Price maybe? I suppose there are cheaper lights that do many of the same things though I haven't seen any with the red & blue light options.
Halifax, Nova Scotia
4 Stars
I would recommend this item to a friend.
Involves a learning curve
July 29, 2014
I got this along with 4 Tenergy RCR123A Lithium batteries and charger. The red and blue LEDs are small but sufficient for close work. The blue LED provides enough light to mind your step in the dark or light up a dark room. The red LED is significantly weaker but sufficient for close tasks. The light output is significant with these batteries and the feel of the flashlight in hand is very nice. The control ring works flawlessly and feels robust. The general impression is one of surprising power and throw in such a small package. My light turned itself off unexpectedly, after a very brief use in turbo mode. However, this happened before I fully charged the Tenergy batteries, and I could not tell whether battery status was the main culprit. This morning I tried again with a fully charged battery and was surprised to see the light turning itself off in less than a minute. I am still trying to figure out if I am using it wrongly. On a medium high setting the light operated flawlessly for 45 minutes -without any noticeable drop in output, and got quite warm to the touch in the process. Clearly, it is not temperature control that turns it off on Turbo. I will return with an update once I figure it out.
Pros:I think it provides a fun element which is not negligible and an unparalleled array functions in its class. This will be my EDC once I figure it out properly. Exceptional delivery service from BJ staff. Thank you.
Cons:Cautious still because of beginner hiccups I am still trying to figure out.
4 Stars
I would recommend this item to a friend.
Great light and lots of features!
July 3, 2014
This is a great little light. I bought it to compare to my EDC, an Olight M10. I thought I'd like all the features of the light. I can say the control ring is awesome. The infinite brightness range is great. The light is well built and nicely finished. The only reason it doesn't get five start from me: There is a very slight perceptible delay to the light coming on when the tailcap is pressed. It makes manually strobing the light a bit tricky. This light has a place on my nightstand on a dimmer setting of brightness. I will keep the Olight M10 as my EDC.
5 Stars
Nitecore rocks again!!
July 23, 2013
I wanted an EDC/ backup pocket light that would work with almost any battery I may have avail on me without worrying about damage. The SRT3 is dead-on it works with AA-CR123-14500-RCR123s and works great!! 550lm on 14500s-RCR123 is no small feat in a compact package. I like the ability to lock the mode you want and use the rear button for fast full power. Definitely a Winner!!! Nitecore has been listening to us flashlight folks. The fact thjis light came with the AA extender tube all in one pkg was one of the reasons I went with Nitecore one stop shopping
Bright Buys
Titanium Innovations CR123A (12PK) 1400mAh 3V 3A Lithium Primary (LiMNO2) Button Top Photo Batteries - Box of 12, Shrink-Wrapped in Pairs$12.00(220 Reviews)As Low As: $10.86 Ea.Free Shipping Over $50!
Nitecore Tube USB Rechargeable LED Keylight - 45 Lumens - Built-in Battery Pack - Black, Blue, Clear, Green or Pink$9.90(139 Reviews)Options AvailableFree Shipping Over $50!
Niteye MSA10 Compact LED Flashlight - 160 Lumens - Uses 1 x AA$54.75(15 Reviews)Free Shipping Over $50!
Duracell Duralock MN1500 AA 1.5V Alkaline Button Top Battery - Made in the USA - Boxed
(12 Reviews)
As Low As: $0.40 Ea.
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Nitecore SmartRing Tactical SRT 3 Defender Flashlight - CREE XM-L2 T6 White LED - Red, Green and Blue LEDs - 550 Lumens - Uses 1 x 14500, 1 x AA, 1 x CR123A or 1 x RCR123A
- $67.46
- This Item Qualifies For FREE SHIPPING
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This item is DiscontinuedClick here to see items you may also like


text:
Holiday Gifts for Foodies (Page 4)
Under $5 per gift:
Fig-Anise Compote
Give your friends the flavors of the season.
The compote is equally delicious as a condiment on a cheese plate, as an accompaniment for roast pork or as a spread on toast. Cook the figs in this compote down until they can be spread with a knife or spoon. If you're giving this as a gift, include a card with serving suggestions.Spiced Nuts »


text:
Lake Zurich, IL, USA < change city >
Ceremony Sites (239)
Reception Sites (172)
Photographers (135)
Florists (104)
Bands (94)
DJs (85)
Wedding Day Beauty (63)
Caterers (55)
Cakes (47)
Limos (36)
Videographers (32)
Invitations (31)
Wedding Fashion (25)
Coordinators (23)
Ceremony Musicians (20)
Rentals (18)
Officiants (17)
Decorations (15)
See All Vendors >
Emily and Brett's Wedding
Ceremony at St. Edna
Reception at European Crystal
Wendy and Jon 's Wedding
Ceremony at Byron Colby Barn
Jackie and Malcolm's Wedding
Ceremony at St Thomas Apostle Catholic Church
Reception at D ' Andrea Banquets
Cayley & Mike's Wedding!
Ceremony at The Metropolis Ballroom of Arlington Heights
Reception at The Metropolis Ballroom of Arlington Heights
Katharyn and Reginald's Wedding
Ceremony at The Jeffreys' House
Reception at The Jeffreys' House
Evie and JD's Wedding
Ceremony at Willow Creek Community Church Chapel
Reception at Willow Creek Community Church
Kathy and Ross's Wedding
Ceremony at St. Frances De Sales
Reception at Radisson Hotel
Sarah and Nick 's Wedding
Reception at Metropolis Ballroom
Laryssa and John's Wedding
Ceremony at St. Volodymyr Ukrainian Orthodox Cathedral
Reception at The Cotillion Banquets
"Our wedding was amazing! The church was beautifully decorated and packed. We also had a professional choir which adde..."
Alice and John's Wedding
Ceremony at St Norbert Parish
Reception at Arboretum Club Banquets


text:
Soccer Moms
by Kathleen Clark
October 9 and 10, 2009
Soccer Moms was the University Theatre entry to the Kennedy Center American College Theater Festival (KCACTF), a national theater education program which aims to identify and promote quality in college-level theater production. The Kennedy Center American College Theater Festival is sponsored in part by the Kennedy Center Corporate Fund, the U.S. Department of Education, the National Committee for the Performing Arts, and the Dr. Gerald and Paula McNichols Foundation.
View photos from the production
Production Team
Director: Pat Farmer
Production Designer: Bruce B. Brown
Makeup: Jenava Moreau
Graphic Designer: Catalina Ruiz-Salazar
Stage Manager: Philip T. Perez
Sound Designer: Bruce B. Brown
Lynn - Kalea Bennett
Alison - Kelsi Ward
Nancy - Rebecca Arnold
Technical Director - Bruce B. Brown
Assistant Stage Manager - Lindsey Humphries
Light Board Operator - Justin Hughes
Sound Board Operator - Clayton Becker
Wardrobe Crew - Jenava Moreau, Brigitte Bridoux, Logan Boone, Jacqueline Jade Jurcik
Props Crew Chief - Lindsey Humphries
Props Run Crew - Houston Hall
Electrics Run Crew - Martin Morales, Andrew Heim, Trey Scott
Stage Crew Chief - Stephen Kennedy
Stage Crew - Elizabeth Malcom, Tori Ann Rhein, Houston Hall, Andrew Heim
House Manager - Angela Butson
Box Office Manager - Holly McPherson
Soccer Coaches - Andrew Heim, Clayton Becker, Trey Scott
Soccer Moms was produced through special arrangement with Samuel French, INC


text:
Princeton University Wind Ensemble
Bob Gravener, conductor
Featuring the world premiere of The Ninth Wave by Nicholas DiBerardino ' 11, guest conductor, plus works by Frank Ticheli, Alfred Reed and more
Richardson Auditorium in Alexander Hall, 8:00 p.m.
Tickets $10 general, $6 students, $5 children 12 and under. Tiger ticket eligible event.
Location: Richardson Auditorium
Date/Time: 05/06/10 at 8:00 pm - 05/06/10 at 10:00 pm
Category: Concerts
Department: Music


text:
Item: DAZ612
720 mg 100 Caps
Serving Size 2 Capsules
Servings Per Container 50
|Amount Per Serving
|% Daily Value
|Turmeric (Curcuma longa) (rhizome)
|1.44 grams
*Daily Value not established.
Other ingredients: Gelatin, magnesium stearate.
Suggested Use: As a dietary supplement, take two capsules once per day with water.
WARNING: Do not take this product if you are pregnant or nursing. Consult your healthcare provider before use if you have or have had gallbladder problems. Discontinue use if you experience gastrointestinal discomfort.
.25 lbs
Turmeric (often pronounced too-mer-ick) is a natural herbal supplement that has its roots in the culinary world... that is until the world discovered its hidden natural health benefits. Traditionally used in cooking as a curry spice, herbalists use turmeric as a liver tonic and gastrointestinal system soother. The key to turmeric's benefits appear to be its high concentration of natural compounds known as curcuminoids, which possess powerful antioxidant capabilities.
"With my personal 100% money-back satisfaction guarantee, you have nothing to lose and great savings to gain."
Lee Swanson, Founder of Swanson Health Products
If you are dissatisfied for any reason, we'll give you a full refund and pay return shipping.
We are dedicated to offering the highest quality products at the best price. Find out more about how we ensure such a high standard in everything we do.Learn More


text:
There is a new series on the Discovery Channel called "Curiosity" that has different topics and is supposed to engage the curiosity and imagination of the viewer. One of the topics in this series has to do with alien invasion. The other day I watched the premiere episode entitled "Alien Invasion: Are We Ready?"
Tag Archive: Signs
Permanent link to this article: http://channelingmyself.com/2011/08/alien-invasion-run-for-the-hills/


text:
Mix all the above ingredients together and bake at 350 degrees for 30 mins or until bubbly
Finally...Home Sweet Home!!
Mix all the above ingredients together and bake at 350 degrees for 30 mins or until bubbly
- 13 Comments
That spaghetti looks yummy! Also looks like a great weekend! Happy belated birthday to your super, cute grandma!ReplyDelete
I LOVE that chicken spaghetti recipe! When I boil my chicken I quarter an onion and bell pepper and throw it in the pot with the chicken. Delish!ReplyDelete
Sounds like a great weekend. I loved the striped dress/top that you wore on Friday night - you looked great!ReplyDelete
Sounds like an amazing weekend!! Busy but fun!ReplyDelete
And you look fabulous in that picture with your grandma!
The chicken spaghetti recipe looks so easy! Thanks for sharing! :)ReplyDelete
Precious pictures! And thank so much for that recipe! My nana makes an amazing tetrazinni...it's kind of her specialty. :) LOVE that striped dress!ReplyDelete
what a fun weekend! and a yummy recipe! ...i was thinkin of your engagement when we were in charleston! its such an incredible place and i'm super jealous that my sister gets to live there and is in college! haha... ohhh the good ole days! :) xoReplyDelete
Sounds like a great weekend! Way cute baby gift :)ReplyDelete
P.S. Head over to my blog to enter my latest giveaway!
Saveli's is delish!ReplyDelete
That chicken spaghetti looks soo good!
Thanks for the recipe! Looks delish! Just found your blog--Looking forward to reading :)ReplyDelete
Love your blog, new follower!! :)ReplyDelete
What a great, jam packed weekend! I agree with you - it's nice to spend a whole weekend in town, though. Hope you enjoy your upcoming free weekends!ReplyDelete
New follower here! I'm lovin your blog.ReplyDelete
I can't wait to read more in the future.


text:
Dungeon Siege 3 - Preview @ RipTen - Demonstration Video
RipTen was present at NY Comic Con conference where Obsidian did a demonstration of Dungeon Siege 3. Their coverage includes both a short write-up of the event as well as a
49 minute tech video demonstration.
Here's a quote from their short write-up about what you can expect:
Video is not working for me. Is there a general problem or specific to my computer?
Video is on vimeo. I have not problem to access it from my workplace.
This looks very different from earlier Obsidian games. The visuals are nice but the gameplay looks like diablo 2/3, which was to be expected I guess? I don't think it'll be my cup of tea but time will tell.
Looks interesting. Dark Alliance II was quite fun co-op, this should be more so. Seems there will be actually a proper story and better gameplay.
I'm worried about the game controls, it looks like they've abandoned the wonderful point & click from the previous games and gone for horrible WASD. If that's the case, then I'll give this game a BIG miss.
WASD? Wow I might actually play it. I did enjoy DS1. DS2 was pretty pathetic. I do like mindless killing and WASD would really sweeten the pot.
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text:
Remove the Hand Icon for Shared Resources (All Windows)
Normally when you share a local resource on the network a hand icon is placed under the item to show it is shared. With this tweak you can remove the icon.
|This tweak can be easily applied using WinGuides Tweak Manager.
Download a free trial now!
Open your registry and find the [SharingHandler] key below.
To remove the icon clear the current data from the "(Default)" value. To restore the icon to the default enter one of the filenames listed below for your OS.
Restart Windows for the changes to take effect.
Value Name: (Default)
Data Type: REG_SZ (String Value)
Value Data: (blank = No Icon, "msshrui.dll" for Windows 9x/Me, "ntshrui.dll" for Windows NT/2000/XP)
>> Recommended Download - check, repair and optimize your registry now with Registry Mechanic <<
Disclaimer: Modifying the registry can cause serious problems that may require you to reinstall your operating system. We cannot guarantee that problems resulting from modifications to the registry can be solved. Use the information provided at your own risk.
Last modified: March 26, 2002
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|Scripting Guide


text:
Hi, in italy we have new tax "web tax"
What is a solution for themeforest???
i think it's only 2 entries for now
i think, you need of more element, gallery grid, price table, some area text (similar single blog post)
Hi, Your static page is a HTML Page ??? Because PHP don't work on HTML page
The issue goes beyond the logic of the columns, is based on a grid 960 so we do not believe there is a need to specify how many columns is formed. In any case thank you surjithctly for feedback. Other suggestions?
thanks kungfu-themes, we'll also be this way. No other suggestion for the PSD version?
thanks manuelvega, any other suggestions?


text:
Editor's Note: This is the fourth in a series of five articles based on the Hydraulic Institute's new Positive Displacement (PD) Pumps: Fundamentals, Design and Applications e-Learning course. To read the previous article, click here. To read the next article, click here.
Last month, we introduced Module 3 of the course, which focused on rotary pumps. In this issue, we will focus on Module 4, reciprocating pumps. For the reciprocating pump family, this article will provide an overview of their characteristics, types, application analysis and pump selection process.
Reciprocating Pump Overview
Since the third century B.C. when an Alexandrian named Ctesibus built a basic hand pump that could transfer water, reciprocating pumps have played a significant role in the development of the modern world. Today, reciprocating pumps have developed into technically advanced machines capable of delivering more than 40,000psi of fluid pressure.
Reciprocating pumps comprise a major segment of the positive displacement technology category. Reciprocating pump designs can pump a full range of liquid types including low viscosity chemicals, high particle content slurries and high viscosity materials. Given this wide operating range, they are often the technology of choice for difficult applications.
One main difference between rotodynamic and reciprocating pumps is that for a given speed, the rate of flow of a rotodynamic pump can be varied from zero flow to a maximum flow. Conversely, reciprocating pumps will have a constant flow for a given speed. Pumps that belong to the reciprocating pump family have several common operating characteristics, including a constant fluid delivery per stroke and mechanical trapping of fluid using suction and discharge valves.
Inherent to their reciprocating motion, these pumps also typically produce pulsation. Consideration for additional devices to reduce pulsation, such as pulsation dampeners or attenuators, may be needed for some applications. Additionally, as with many PD pump types, systems may require overpressure relief protection. Efficiencies of reciprocating pumps vary widely across the category due to driver types and mechanical configurations.
Types of Reciprocating Pumps
Four common types of reciprocating pumps-power pumps, power diaphragm pumps, air operated diaphragm pumps and air operated piston pumps-are reviewed in the reciprocating pump module.
Power pumps are reciprocating machines where plungers or pistons are driven within a valved cylinder by a power end. The power end (see Figure 1) converts the rotary motion of a motor-electric, air or hydraulic or diesel engine-into reciprocating motion by means of a crankshaft, connecting rods and crossheads. The liquid end (see Figure 2) connects to the power end and contains the plungers, packing, fluid chambers and valves.
The reciprocating motion of the plunger in concert with the other liquid end components can develop more than 40,000psi of fluid pressure or more than 4,000gpm of fluid flow. These pumps typically have one, two, three, five, seven or nine connecting rods and crossheads that drive an equal amount of fluid plungers. Configurations of an odd amount of cylinders are preferred to reduce pulsation. Pulsation is generated by the oscillating pressure of each fluid chamber from suction pressure to discharge pressure. The oscillating pressure, cycling at 50- to 500-rpm, is a popular driver of pump failures, but these pumps are constructed robustly to resist fatigue.
It is important to control pressure with power pump systems. As the pump injects the displaced liquid into the discharge system, the pressure is increased. The pressure will continue to increase until it meets the requirement of the system. However, if the system pressure requirement is not controlled, the pressure will continue to build up until something ruptures in the system or the pump or the driver of the pump stalls out. Power pumps should be equipped with a pressure relieve device to prevent the over-pressurization of the system beyond its recommended limits.
Power pumps are typically used for low viscosity chemicals, oils, high pressure cleaning, ore slurries, drilling mud, reverse osmosis, saltwater injection, hot oil applications, blow out preventers and subsea applications.
Air operated diaphragm pumps combine the reciprocating motion of a flexible membrane, called a diaphragm, with check valve mechanisms to transfer fluid. These pumps typically have two diaphragms connected to a reciprocating shaft in which one side of the diaphragm is in contact with the liquid being pumped and the other side is in contact with the compressed air. The reciprocating motion is created by an air motor that alternates pressurization of the two diaphragms. Fluid pressure is usually equivalent to the supply air pressure of the pump; however, amplification devices allow specialized pumps to operate at pressures up to three times the supply pressure.