text:
|Jun, Won
|Bhagwat, Arvind
|Gross, Kenneth
|Smith, Allen
|Angle, Scott
Submitted to: American Society for Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/23/2004
Publication Date: 5/23/2004
Citation: Jun, W., Bhagwat, A.A., Gross, K.C., Smith, A.D., Angle, S.J. 2004. Membrane-derived oligosaccharides of Salmonella enterica serovar Typhimurium. American Society for Microbiology Annual Meeting. p. 107. Interpretive Summary:
Technical Abstract: Membrane-derived Oligosaccharides of Salmonella enterica serovar Typhimurium: Membrane-derived oligosaccharides (MDO) consist of branched substituted ß-glucan chains that are present in the periplasmic space of Escherichia coli and other gram-negative bacteria. Their common features are the presence of glucose as the sole constituent sugar and their increased levels in low-osmolarity media. In E. coli, the MDO backbone, containing 7 to 13 glucose units is synthesized by the products of the modGH operon. In several phytopathogenic bacteria, mutants defective in MDO synthesis failed to incite disease on the host plant. MDO from Salmonella species have not been studied for their role in virulence or osmotolerance. We fractionated and purified MDO from S. enterica serovar Typhimurium and performed glycosyl composition analysis (mol%: 9:1:1, glucose : galactose : mannose, respectively). Using a gene specific mutagenesis protocol, mdoG and mdoH mutants were generated and examined for MDO synthesis. The mdoH::kan mutant had no effect on MDO biosynthesis, while mdoG::kan mutant synthesized rhamnose-substituted MDO with altered composition (mol%: 2:1:1:7, glucose : galactose : mannose : rhamnose). Unlike mdoGH mutants of Xanthomonas and Erwinia spp., the mdoG mutant of S. enterica serovar Typhimurium suffered only a marginal loss of virulence when tested in BALB/c female mice. Although, addition of phosphoglycerol residues to MDO (function of mdoB) of S. enterica serovar Typhimurium has been shown to be essential for virulence, our data indicate that rhamnose substituted MDO molecules may provide essential virulence functions.


text:
Kate Young's New Target Line
Posted Monday January 21, 2013 6:55 PM GMT
Photo Credit: Getty Images
Kate Young rose to fame as the stylist for Natalie Portman and Michelle Williams, and now she's gearing up for her very own line to be sold in Target stores.
Priced from $14.99 to $89.99, Young's wares will hit the shelves on April 14, though there's already a plethora of buzz regarding what's to come.
The talent designer has plenty of experience working with a variety of labels such as Jason Wu, Armani Exchange, Ann Taylor, and Derek Lam, giving her a wealth of inspiration from which to draw.
Last year Young teamed with Cardigan for a knitwear capsule collection, as well as a mainstream range for Macy's INC label.
As for her Target lineup, fans can expect party dresses, casual separates, shoes and accessories.

subset: commoncrawl


text:
Toward non-invasive detection of concealed energetic materials in-field under ambient-light conditions
Cletus, Biju, Olds, William J., Izake, Emad L., Fredericks, Peter M., Panayiotou, Helen, & Jaatinen, Esa (2011) Toward non-invasive detection of concealed energetic materials in-field under ambient-light conditions. Next-Generation Spectroscopic Technologies IV, 8032, 80320I-1.
Submitted Version
(PDF 301kB)
View at publisher (open access)
Spatially offset Raman spectroscopy (SORS) is demonstrated for the non-contact detection of energetic materials concealed within non-transparent, diffusely scattering containers. A modified design of an inverse SORS probe has been developed and tested. The SORS probe has been successfully used for the detection of various energetic substances inside different types of plastic containers. The tests have been successfully conducted under incandescent and fluorescent background lights as well as under daylight conditions, using a non-contact working distance of 6 cm. The interrogation times for the detection of the substances were less than 1 minute in each case, highlighting the suitability of the device for near real-time detection of concealed hazards in the field. The device has potential applications in forensic analysis and homeland security investigations.
Impact and interest:
Citation counts are sourced monthly from Scopus and Web of Science® citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.
Full-text downloads:
Full-text downloads displays the total number of times this work's files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
|ID Code:
|Item Type:
|Journal Article
|Additional Information:
|Proceedings of SPIE
|Inverse SORS, Raman spectroscopy, energetic materials, non-contact analysis, in field detection, forensic analysis, homeland security
|Australian and New Zealand Standard Research Classification > PHYSICAL SCIENCES (020000) > OPTICAL PHYSICS (020500)
Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000)
Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)
|Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:
|Copyright 2011 Society of Photo-optical Instrumentation Engineers
|Deposited On:
|30 Apr 2012 03:39
|Last Modified:
|30 Apr 2012 20:15
Export: EndNote | Dublin Core | BibTeX
Repository Staff Only: item control page


text:
|Home » News »
|Tuesday, June 27 2017
Newsday Archives
Every day fresh news
A d v e r t i s e m e n t
A d v e r t i s e m e n t
click on pic to zoom in
Chief Justice Ivor Archie...
CHIEF Justice Ivor Archie minced no words. On January 15, he reprimanded an attorney who brought a lawsuit all the way to the Court of Appeal on the basis of information which reportedly came from a witness whom the lawyer had never met, never seen and didnt even know the name of. The anonymous witness was also, apparently, invisible.
How could you swear an oath, sign a certificate of truth, if you do not know the source of the information contained in it? Archie asked the lawyer Taurean Dassyne and his instructing attorney Sylesh Ramjattan. Also on the Court of Appeal in the case that day was Justice of Appeal Nolan Bereaux. He asked Dassyne, Do you know who the witness was? You must know who the witness is.
A d v e r t i s e m e n t
Top stories
Pictures & Galleries
The Ch@t Room
Click here to tell us right now!
Crisis Hotline
|Copyright © Daily News Limited | About us | Privacy | Contact
IPS Software by Agile Telecom Ltd
Creation time: 0.165 sek.


text:
Your browser does not support iframes.
Each time the calendar flips to the month sporting the annual Men's Health Fair at Jewish Hospital Shelbyville, the event serves as a vivid reminder for its chairman, Tony Carriss.
Information was gathered from previous years of The Shelby Sentinel, The Shelby News and The Sentinel-News. You can reach the writer at email@example.com.
Normal 0 0 2010-06-08T20:29:00Z 1 559 3190 26 6 3917 11.517
The Shelbyville City Council will hear a budget-amendment ordinance on Thursday night that accounts for the city's spending a little more money this year and saving a whole lot in the future.
The city has spent $880,000 from its reserves fund to pay off the mortgage on city hall and another $285,000 to pay off its portion of the Family Activities Center at Clear Creek Park.
THE Sentinel-NEWS: How did this idea originate?
The World Cup of soccer begins this week, and all eyes will be fixed on the competition in South Africa.
At least that's what they tell me.
I hear it on ESPN, read about it on the cover of Sports Illustrated and see it promoted in multicultural eating establishments around the area.
This is said to be the biggest and greatest sports event in the world.
Triple S meets Tuesday
Normal 0 0 1
Copyright © 2017
The Sentinel-News is your source for local news, sports, events and information in Shelby County and Shelbyville, KY, and the surrounding area.


text:
Not everyone makes the same choices when it comes to reading blogs. There are those that keep blogrolls on their site and read blogs by clicking on the links. Most of us know that is quite inefficient, especially if some people hardly ever update their blogs while others update every day (or worse, they update haphazardly, at unpredictable times).
Assuming if you read blogs regularly and you want to subscribe to a blog, how do you prefer to receive your subscription? Personally, I have been using Google Reader for many years, and I go there when I feel like or have time to read blogs. Many years ago on another blog I wrote a post about Google Reader – if there is interest, I can rewrite it for this blog. I can't understand why anyone would want their email cluttered with email subscriptions. But obviously many feel differently! Actually, I'm guessing that most of you reading this post prefer email – but using the poll at the bottom, you can prove me wrong.
A few weeks ago a kind reader requested a subscription by email. To make a longish story short, I have now set up an email subscription for this blog using Mail Chimp. You can also click on the Subscribe by Email link on the top right corner of this Websites for Small Biz blog. If you want to learn more about MailChimp, why, Jeri wrote a great post on the MailChimp service! I will just add that I had read users were unhappy with Feedburner, so I did my research, and MailChimp is indeed a good way to go for mailing lists and email newsletters.
I will finish this post with a poll (poll will be open until Feb. 27th):
Your Turn, Please
If you use an RSS reader (example is Google Reader), which one do you use and why? If you use a service for converting your blog RSS feed to email, which service do you use? If you like the idea of subscribing to blogs but don't really understand the difference between these two methods, would you like more explanation?


text:
Leave a Reply
You must be logged in to post a comment.
Conversations shaping the future of emerging media
Bill displays strong emphasis on indie rock, returning acts
More here:
Coachella music fest announces lineup
You must be logged in to post a comment.
Talk NYC/WW is your daily download of the tech, marketing and advertising news you need to know. It's smartly curated to keep you up to speed on the innovators and innovations that are shaking up the digital world today.


text: Hey guys, I have an 02 which doesn't stop to my liking. I feel like I have to push the pedal to the floor to get descent stopping power. It doesn't feel like it needs the brake lines bled, just a lack of grip. Are there any suggestions to this? My fiancee's 03 accent nearly puts me through the windshield when I drive it because it brakes awesome. Thanks!


text:
November 2−December 19, 2004
This UAM is pleased to present a solo exhibition of work by Los Angeles−based artist Mungo Thomson. It is the 65th exhibition in the Centric program, an on−going exhibition series dedicated to displaying new work and introducing emerging national and international artists to new audiences. Mungo Thomson's work in a variety of media utilizes mass cultural vernacular to explore the phenomenology of social space and the mechanics and poetics of reception. Thomson's Centric exhibition will include two new 35mm film projects and a new multi–channel video installation, each preoccupied with the history and practices of Hollywood film production and movie going. Also on view will be a new sound project exploring the practical social context of minimalist art. The installations include The Bootleg Series, Cinema Concepts, New York, New York, New York, New York and The Swordsman.


text:
Wicked Stick is the only John Daly signature course in the USA, and he has teamed up with golf course architect Clyde Johnston to help him create a "Grip It and Rip It" off the tee golf course that challenges even the long ball hitter. The most recognized hole is No. 7, a 265-yard par 3 monster, in a location where wind is its own hazard. Golfers enjoy the water and spacious waste bunkers, because there is always a way out.
Wicked Sticks Golf Links has recently earned an overall rating of 7.9 from reviewers at the Myrtle Beach Golf Insider, garnering praise for course layout, friendly staff, and course conditions.Book Tee Times Now> Request A Quick Quote>
|Rates for June 26, 2017
|Course Reviews
Best Rate7:00-8:29 AM
Best Rate8:30-11:59 AM
Best Rate12:00-1:29 PM
Best Rate1:30-6:30 PM
Best Deal


text:
- Healthy Eating
- About Our Products
- Recipes
- Online Ordering
- Our Mission & Values
- Blogs
- Store Departments
By Elizabeth Beal, June 5, 2013 | More posts by Elizabeth Beal
Sure, juicy steaks, sticky banana splits and tubs of craft beer may be every father's cookout fantasy, but it's possible to make dad a special meal that tastes fantastic and won't leave him in a stupor.
We've rounded up our ten favorite healthy recipes for daddy-o's to enjoy on their day (psst – that's Sunday, June 16th).
What healthy and delicious treats will you cook for the dad in your life?
To provide feedback or ask a question about our company, a store or a product, please visit our Customer Service page.
For more information about posting comments to our blog, please see our Comment Posting Guidelines.
6 comments
Lisa says …
Donna Dyett says …
jjferguson1 says …
Nikki - Community Moderator says …
God'sCreation says …
Debbie Kolaski says …
Welcome to Whole Story, the official blog of Whole Foods Market.As the world's leading natural and organic grocer we're all about healthy food and a healthy planet, and we've got bunches of passionate people in our community doing incredible things. Join in the conversation!
Selecting a store allows you to see that store's content throughout the site, such as sales, store events, and more.
Receive recipes news and tips.
Sign up
Copyright 2017 Whole Foods Market IP. L.P.
Terms of Use | Privacy Policy | Supply Chain Disclosures | Internet Based Ads | CA Privacy Rights
Site Map | Site Information
"Whole Foods Market" is a registered trademark of Whole Foods Market IP, L.P.
We have sent a confirmation email to {* emailAddressData *}. Please check your email and click on the link to activate your account.
Please confirm the information below before signing in. Already have an account? Sign In.{* #registrationForm *} {* traditionalRegistration_firstName *} {* traditionalRegistration_lastName *} {* traditionalRegistration_emailAddress *} {* traditionalRegistration_password *} {* traditionalRegistration_passwordConfirm *} {* traditionalRegistration_displayName *} {* traditionalRegistration_postalCode *} {* captcha *} By clicking "Create Account", you confirm that you accept our terms of service and have read and understand privacy policy.
We've sent an email with instructions to create a new password. Your existing password has not been changed.


text:
I put this letter together to present to a private club I am a member of. It is intended to be a more comprehensive argument for an establishment that you already frequent and is of some importance to you.
It remains to be seen if it will be effective, but I have received compliments on the presentation from several attorneys (not sure how I feel about that).
Feel free to use some or all of it in your adventures. You'll need to make the obvious changes to fit your state and circumstances.
I am writing to address the issue of the new club rule barring admittance to CCW permit holders unless they disarm before entry. I see that the web site is long out of date, so I ask that you pass this message along to the BOD members who are not listed above.
Based on the tone of the membership meeting, I strongly suspect that BOD members have neither completely read the CCW law nor comprehend the potential legal impact of their decision. The path you are taking may place the club in a position of serious liability risk.
Because of my lifelong interest in the topic, I have followed issues related to firearms and firearms law for decades. In my opinion, the decision to bar armed CCW holders has the potential to set the stage for a crippling civil lawsuit.
I very strongly suggest that you seek legal advice before you finalize this decision. Please consult a competent civil law plaintiff's attorney to get an outside opinion on the matter. I will suggest that a personal injury specialist will offer the best perspective from the standpoint of a potential injured party litigant.
The public debate that took place during the crafting of this law was extensive and comprehensive. There is a tremendous amount of information available through the Senate and Assembly records as well as an excellent synopsis at the DOJ web site. If you read through the materials below, I believe that you will at least have a starting point to do you own research towards an informed decision.
I would first direct your attention to Section 21 (Immunity) of the 2011 Wisconsin Act 35. http://docs.legis.wisconsin.gov/2011...ed/acts/35.pdf This clause renders any establishment that ALLOWS CCW on its premises immune from any liability arising its decision.
In short, the law immunizes the club against any civil liability that might arise from any action or incident that involves a CCW holder using or displaying a firearm. In any grievance of this nature, the club would be legally excluded from civil action by any party. Section 21 eliminates ANY AND ALL legal and/or financial reasons to bar armed CCW holders from the premises.
For those businesses choosing to bar entry to armed CCW holders, the impact appears to be the opposite. Section 21 is written to specifically discourage establishments from barring armed CCW permit holders. The legal creation a specific class designated as immune from liability by its very existence creates a separate class that is not immune.
By barring CCW in the club, the BOD may expose the club to potential civil liability for a loss experienced by a disarmed permit holder (or other victims if a permit holder was present) while on the club property. This means that a rape, assault, robbery or similar incident that might have been stopped or prevented by a permit holder may become a matter of potential liability for the club.
While not yet tested in Wisconsin, the chances are very strong that some of these cases will be allowed to move forward in the civil courts. The nature of Section 21 creates a new set of legal possibilities for the trial attorneys to get rich on. One has to wonder how a jury will react in such a case.
Another issue to consider is the message sent to criminals in search of easy victims. As this new law comes into force, tens of thousands, possibly hundreds of thousands of Wisconsin residents will be going armed. Criminals normally accustomed to preying on helpless unarmed victims will be reassessing their options.
One result of the publicly posted warning signs is to tell criminals that all persons entering or leaving the establishment will be unarmed. Unfortunately, evidence from other states indicates that posted "Gun Free" areas become magnets for criminals seeking helpless victims. They make a point of targeting "Gun Free" establishments, especially the publicly accessible parking lots serving such places. Why take a chance on anyone on the street when you have guaranteed "safe" targets in the xxxxxx Club parking lot?
I will offer several items for consideration.
When Florida enacted its CCW law in 1987, opponents issued dire warnings of an inevitable "wave of violence" on the streets. The battle cry of those opposed to the law was that Florida would become the "Wild West". The airwaves were flooded with hysterical warnings that the streets of Miami and Tampa would become "rivers of blood". As we know, such apocalyptic occurrences did not take place.
Over the last 25 years, nearly every state that did not already allow CCW has enacted laws permitting it. In every state opponents have trotted out the Wild West argument, only to see their prophecies of rivers of blood come to nothing. In state after state, year after year, this ridiculous argument is presented by CCW opponents. In state after state, year after year, the predictions of waves of murder and violence have not been fulfilled.
Please consider further.
A CCW holder in Wisconsin, by law;
Has no felony convictions;
Has no domestic violence convictions;
Has no restraining orders or restrictions;
Has no history of mental health commitments or judicial notice of mental health issues;
Has passed a state mandated training program;
Has passed a background check by the State Department of Justice.
In addition, national statistics indicate:
CCW holders are among the most law-abiding in the nation;
CCW holders are less likely to be arrested for a crime than a law enforcement officer;
CCW revocations for firearm crimes or permit violations are statistically near zero.
The facts are very clear.
Section 21 protects the club with legal immunity. This eliminates ANY possible argument on the basis of potential liability.
Statistical evidence from around the country proves beyond any doubt or question that CCW holders are less likely to commit a gun related crime than virtually any other class of citizen.
The Wisconsin DOJ background check virtually eliminates the possibility of a felon, domestic violence offender or seriously mentally ill person obtaining a permit.
The simple reality is that the ONLY people who will respect your ban are law abiding CCW holders. No person carrying illegally is going to respect your wishes, now or later, ban or no ban. At best, criminals will ignore your sign. At worst, it will lure them to the easy pickings in the club parking lot, or even inside.
In short, there are no logic or fact based reasons to bar armed CCW holders from the club. I realize that some people disagree with the notion that anyone should be armed, or even own firearms, but those opinions are based in emotion, not in fact.
I understand that the BOD has the right to make such a decision, but they also have a requirement to perform due diligence before finalizing such a decision. An issue like this can be very contentious, with strong opinions clouding good judgment.
From some of the comments floated by BOD members at the meeting, it seems that there are some very strong opinions held by some on the Board. I respectfully submit that your duty to the club and its members is to approach this situation in your role as elected officers. Personal preferences should not play any role is such an important matter.
I ask that the BOD members revisit this issue and decide it based on facts instead of emotional bias. I am available for further discussion on the matter. I am willing to provide data resources from both sides of the issue so you can judge the merits of the respective positions for yourselves. Above all, I repeat my advice to seek competent legal advice on this issue. It is much to important to handle without expert help.
Andrew H Litkowiak
Annual Member


text:
Often times we will buy a stock after we hear a compelling story from some analyst or from an executive from the company. They make it seem as if you are guaranteed to make money when you buy a stock. But this simply isn't so. At best we can try to put the odds in our favor and even then it take a lot of hard work that requires you check your emotions at the door. So before you decide to pull the trigger, ask yourself these three questions.
1. Who's selling and why?
One thing that people forget when they are buying a stock is that there is always somebody on the other side of the trade—somebody is selling the stock to you. We often forget this point, and by extension we forget that the person on the other side of the trade has a reason of his/her own for selling. Now this reason may have nothing to do with the fundamentals of the underlying company.
For instance the stock may have risen a lot and the investor wants to rebalance his or her portfolio. He or she may also need the cash. But it is also possible that the owner of the stock bought the stock at a lower price in anticipation of the same story that is now prompting you to buy it at a higher price. It may also be a seasoned short-seller who is borrowing stock from his or her broker as a result of extensive research into the aggressive accounting strategies that management relies upon to sucker new investors in at an irrationally high price. While we can never know for sure we should at least take a guess, and more importantly, understand the bear case.


text:
Upstream / Downstream
Explore pathways related to this product.
Research More. Spend Less.
Spend $650 or more and get 20% off*
(*Offer valid in the US only. Expires June 30, 2017)
To Purchase # 3057S
|20 µl (2 western blots)
|100 µl (10 western blots)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
NEK7 (C34C3) Rabbit mAb #3057
Western blot analysis of GST-NEK6 and GST-NEK7 fusion proteins and extracts from HeLa and Jurkat cells using NEK7 (C34C3) Rabbit mAb.Learn more about how we get our images
Gallery: NEK7 (C34C3) Rabbit mAb #3057
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Bovine Serum Albumin (BSA): (#9998).
- Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 5 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised October 2016
|Western Blotting
NEK7 (C34C3) Rabbit mAb detects endogenous levels of total NEK7 protein.Species Reactivity: Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human NEK7.
The NEK family of protein kinases is composed of 11 members in humans that share an amino-terminal catalytic domain related to NIMA, a serine/threonine kinase identified in Aspergillus nidulans. While NIMA is critical for cell cycle progression in fungus, the function of NEK kinases in mammalian cells is largely unknown. NEK1 was first identified by screening mouse cDNA expression libraries and was demonstrated to have dual specificity kinase activity on both tyrosine and serine/threonine sites (1). NEK2 most closely resembles fungal NIMA in its primary structure and is believed to promote the splitting of duplicated centrosomes at the onset of mitosis (2,3). NEK3 is predominantly a cytoplasmic enzyme and its activity shows marginal variation throughout the cell cycle (4). NEK4 is ubiquitously expressed and its expression and subcellular location are not associated with cell cycle (5). NEK6/7 have been suggested to phosphorylate and activate p70 S6 kinase in vitro (6). Expression of an inactive NEK6 mutant arrests cells in M phase and interferes with chromosome segregation (7). NEK8 activity is not cell cycle regulated and may play a role in cell cycle independent microtubule dynamics (8). NEK9 is activated during mitosis and may participate in the activation of NEK6/7 during mitosis (9,10).
1. Letwin, K. et al. (1992) EMBO J. 11, 3521-3531.
2. Fry, A.M. et al. (1995) J. Biol. Chem. 270, 12899-12905.
3. Fry, A.M. (2002) Oncogene 21, 6184-6194.
4. Tanaka, K. and Nigg, E.A. (1999) J. Biol. Chem. 274, 13491-13497.
5. Hayashi, K. et al. (1999) Biochem. Biophys. Res. Commun. 264, 449-456.
6. Belham, C. et al. (2001) Curr. Biol. 11, 1155-1167.
7. Yin, M.J. et al. (2003) J. Biol. Chem. 278, 52454-52460.
8. Holland, P.M. et al. (2002) J. Biol. Chem. 277, 16229-16240.
9. Belham, C. et al. (2003) J. Biol. Chem. 278, 34897-34909.
10. Roig, J. et al. (2002) Genes Dev. 16, 1640-1658.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063.


text:
Wednesday, January 23, 2013
Where: 627 Dartmouth Place, Searle Center Library, Evanston, IL 60208 map it
Audience: Student
Tom Popelka
Group: Searle Graduate Events
Category: Academic
Undergraduate Lab Reports: Grading and Improving Student Learning
Facilitator: Jennifer Hobbs
When: Wed 1/23, 10:30 AM – 12:00 PM
Where: Searle Library
The structure of undergraduate lab reports can vary tremendously among departments and course-level. Regardless of specific structure, all lab reports should help students learn to organize, analyze, and present their data, and use this data to draw conclusions and support claims, explain their scientific understanding clearly in words, and reflect on their experimentation. As TAs, grading lab reports can be laborious, and time constraints make it difficult to provide students with opportunities to learn from and improve on their mistakes. In this workshop we will explore ways to improve speed and efficiency in grading these reports while providing students with adequate feedback and means of improving their learning in the process.
Jennifer Hobbs is a fifth year graduate student in the Physics department doing computational and behavioral research on the neuromechanics of the rat whisker system. She is currently a Graduate Teaching Fellow and Teaching Consultant and has previously served as a Graduate Teaching Mentor (2011-2012) and TA Fellow (2009, 2010, 2011) through the Searle Center. Jennifer has been a TA for a number of the introductory physics courses (Physics 125-3, Physics 130-1,2,3, Physics 135-2,3) as both a discussion and laboratory TA and was an HHMI Teaching Fellow for the NuViBE program in 2011-2012, helping to redesign the Bio210 sequence.


text:
Ardan Studios has helped many clients get where they want to go by delivering captivating applications focused on customer goals. Review some of our recent projects below or contact us today.Get Started
GothamGo is a celebration of the Gopher community on the East Coast and beyond. Featuring serious discussion, networking, knowledge sharing, and thoughtful presentations by some of the world's best Go developers.View Project
Bio-Path is a biotechnology company focused on the clinical development of therapeutics by utilizing DNAbilize™, our novel and patented method for producing antisense DNA therapeutics for a broad spectrum of indications, including cancer.View Project
GopherCon, is the largest conference dedicated solely to the Go programming language. It is held yearly in Denver, Colorado.View Project
An app for iOS that provides realtime location based weather information. This includes weather from NOAA and other data sources for buoy, tide, marine forecasts and observation stations. Fishermen and outdoor enthusiasts love it!
GopherCon, is the largest conference dedicated solely to the Go programming language. It is held yearly in Denver, Colorado.View Project
Lupa Shoes is a complete ecommerce website with over 50,000 products. Completely responsive and integrated with point of sale.View Project
DockerCon 2015 was a two day Docker-centric conference that was organized by Docker, Inc on June, 2015 in San Francisco, California.View Project
A transaction level data technology company offering an evolved personal financial management (PFM) solution designed to provide a proactive – rather than reactive – approach to personal financial management and credit data.View Project
Allows businesses track their field workers with ease. Manages work orders and customers with an iOS app and web application.View Project
Fiercely passionate about design and technology our team is willing and waiting to work on your latest new idea.
Drop us a line so we can chat about your ideas and what you are looking to accomplish.Get Started
We are excited to hear from you! Contact us today.


text:
Off Court Hits and Misses: Novak Djokovic
Tennis players are constantly the subject of scrutiny when it comes to what they wear on the court. Male or female, if you're a top player, you can bet everyone will be giving the thumbs up or down to your Nike duds when a Grand Slam rolls around. Looking at on-court photos, we can deduce that the likes of Serena Williams, Maria Sharapova and Roger Federer all have spectacular style.
But do they really?
Off-court might tell a different tale. Free from the restrictions of sponsorship, tennis players can choose exactly what they want to wear. And that's not always a good thing.
On The Go Tennis will be analysing the off-court style of a top player every week. Shoes, earrings, cardigans... nothing will go unnoticed.
Novak Djokovic is the joker of the ATP; from impersonations to karaoke, he knows how to make fans giggle. No doubt he also likes to look cool doing it; no one wants to be laughed at for the their taste in clothing. So does Djokovic manage to look good when he's having a laugh away from tennis? Or is his fashion sense just as funny as his dancing?
His best look
For someone who attracts so much attention for being the life of the party, Novak might be expected to look best showing off the latest trends. I half expected to see him in short pink shorts, heavily gelled hair and a singlet cut so low it could be a woman's, (that's the fashion here in Australia, I don't know about you lot). Thankfully, Djokovic steers well clear of such outfits. Instead of wearing Justin Bieber inspired low-crotch trousers, he parties in tidy collared shirts and straight-legged jeans. The effect is such that Novak maintains an air of mystery; he looks prim and proper, but we all suspect he goes pretty wild behind closed doors. Which is pretty damn sexy. By choosing modest shirts, Novak doesn't scream 'look at me.' Instead, he knows he can walk into a room and stop it without having to wear anything outrageous.
And stop it he does...
Ok, but seriously, he looks good when he hasn't already been dancing for hours.
Personally, I can't resist a man who knows how to tuck his shirt in without looking like an absolute dork.
His worst look
It isn't all good when it comes to Novak's style. Fortunately for his repuatation, the biggest fashion sins appear to have been committed by stylists, not Novak himself. Whenever Novak is posing for a magazine, something called "White Pants Syndrome" overcomes those dressing him. Before Novak knows what's hit him, he's been dressed in the tightest white jeans they can squeeze him into and instructed to lie back and pull at his crotch.
Like any other man eager to be liked, Novak does what he is told.
My, what a large trophy you have, Novak.
White pants may be alternative and modern, but they're also very camp. They can only be worn with flair by the likes of Michael Jackson and Kate Middleton, and Novak Djokovic possesses neither the class nor cool to pull these off. Barely any man does.
But that's Ok, he looks pretty good without any pants at all. And how many people can say that?
His accessories
Glasses have made a huge comeback recently; hipsters with 20/20 vision are regularly spotted without lenses in their frames, (and despised by those who suffered years of being called '4 eyes' by those same hipsters in primary school). But those who suit glasses manage to look bookish in a sexy way and are the envy of fashionstas worldwide. Novak Djokovic is one of those people. He may be regulated to contact lenses on the court, but off court he rocks those frames as if he is the muse behind all glasses-wearing hipsters.
Glasses may be fashionable now, but many people feel uncomfortable wearing them. We praise Novak for choosing to wear them on a regular basis, giving a confidence boost to glasses-wearing tennis fans worldwide.
Even his girlfriend, Jelena Ristic, gets in on the action occasionally.
For the most part...
Novak dresses himself in a smart but relaxed style. Keeping it sensible is a good choice for this extroverted No.1; no one likes a show-off. However, he does need to learn how to resist peer pressure and have a say in what he wears on photo-shoots, (I am giving him the benefit of the doubt and assuming he is forced into white jeans). Nobody likes a pushover, Novak. Just tell them that trophies that are positioned in a phallic manner and tight white pants aren't really your thing.
Some more of our favourite Novak moments...
Love it? Hate it? Let us know how you think Novak should dress in the comments below.
-Kait O'Callahan


text:
some pics of my new car 330ci sport
some pics of my new car 330ci sport
This forum loves members who own cameras and are not afraid to use them!
Hi macca1995,Welcome to bimmerforums.co.uk...........
Car looks cool
welcome matey nice car
Welcome, nice motor
very nice!!
Good Choice
Lovely car , I also recently bought my first BMW E92 330i . I often thought previous shape coupe (like yours) actually looks nicer than current model although current shape is gradually growing on me.
Hope you enjoy yours as much as I'm enjoying mine
|« Previous Thread | Next Thread »
|Tags for this Thread


text:
Ripple Glass Pendant - Oval
- Overview
Ripple effect. Gather under the glow of our gently-textured Ripple Glass Pendant, featuring a glass oval with a slender metal base.
- 18"diam. x 18.5"h.
- Clear glass.
- Polished Nickel-finished metal frame.
- Hardwired.
- Imported.
- Details + Dimensions
- Overall product dimensions: 18"diam. x 18.5"h.
- Shade dimensions: 18"diam. x 12.5"h.
- Length from lamp to ceiling adjusts incrementally from 18.5" to 55" (includes three 12" rods and one 6" rod).
- Canopy dimensions: 5"diam.
- Accommodates a 9W CFL or a 40W incandescent bulb.
- UL listed.
Hardwired; professional installation recommended. Can be hung from a sloped ceiling.
Wipe clean with a damp cloth.
- Shipping + Return Info
These items ship from our Distribution Center by UPS and arrive in your home within 4-5 business days of order receipt.
Shipping Rates
The shipping rate varies depending on your order total and shipping options. View Shipping Rates and Options
Next Day Delivery
For an extra charge of $17.50 per address, we can arrange for rush delivery to most destinations.
We happily accept returns on stocked items within the time frames listed below. Products designated as special order (SPO) cannot be cancled or returned. All items on our website and in-store are considered 'stocked' unless expressly identified as 'special order' instead. Prior purchases are not eligible for price adjustments. Please familiarize yourself with our returns policy before placing your order.
Stocked Items: If you are dissatisfied with your purchase of a stocked item, you must exchange or return it within the following time frames for a full refund of the merchandise cost (delivery fees are non-refundable). Outside these time frames, we will replace or refund your purchase against manufacturer's defects ONLY. Please note that we cannot accept returns on gift cards or items damaged through normal wear and tear. We also cannot accept returns of final-sale items, which are identified by a price ending in .99 or .97.
- Quick Ship upholstery items must be returned within 7 days of the order receipt date.
- All other stocked products must be returned within 30 days of the order receipt date.
Special Order Items (SPO): Because they're custom made to your specifications, special order items may not be canceled or returned and are exchangeable against manufacturer's defects ONLY. SPO includes special order upholstery items, monogrammed/personalized items, special order rugs, one-of-a kind rugs and any products being shipped directly from a third-party vendor. Any special order items will be flagged as 'special order' in your shopping cart. NEW JERSEY CUSTOMERS: Special-order upholstered furniture returns are subject to applicable state law and are non-returnable after 24 hours of delivery.
For further assistance please call our customer service department at 1.888.922.4119 or send an email to email@example.com


text:
Hey guys,
first of all… AWESOME site! I've always enjoyed working with flash and it's a real pleasure for me to come up with new ideas and share it with people here! Been a member for about a week now and I just broke the 100$ sold.
Now please help me become exclusive as fast as possible will ya? Good thing I don't have so much to do at work right now so I might start building some bigger projects like xml-driven templates or gallerys soon.
Wish you all a nice day


text:
Site is here http://socialmediasuperfans.com/ it is running on Elegance Storefront ThemeThe plugin I purchased is here http://codecanyon.net/item/usquare-universal-responsive-grid-for-wordpress/3352677
Need this installed correctly so that 9 items show and perform correctly .
Please let me know rates and turn around time.
I've been able to get the short codes to show up but to get this to perform correctly it calls for editing the php files and I don't quite have the time to do this.
I should be able to do this now – please contact me here


text:
1952 Red Hopalong Cassidy lunchbox set w/Thermos
- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Toys, Dolls, Games & Puzzles
- Source: eBay
- Sold Date: Nov 14, 2006
- Channel: Auction House
1952 Hopalong Cassidy Lunchbox and Thermos in very good( USED condition )for its age. I rate this set as an 8.0 or better out of 10 . 10 being Mint (perfect).It is used with normal rim wear and a little rust and t The thermos is complete. This is a great set for any collection.And it still has Price sticker on it !!WOW !! Is'nt that Cool. If you need more Info please email me .Thanks for looking.Check out my other items !Be sure to add me to your favorites list ! All items are sold as is. Please e-mail if you have questions or concerns regarding the item. Photo will display the item, but it does not always give you the complete story so always contact the seller before bidding. I will combine shipping for multiple items


text:
82 Great Eastern Street
London EC2A 3JF
United Kingdom
View the venue listing
Invite friends
Line-up /
- Event admin /
- DeanTyler
- Update this event
Dean Tyler, Montana Cruz, Digital Thrills, Jon Sojo, Special Guests
DEFINITION has been having a memorable recent months which included summer cruises, worldwide known headline DJs, fantastic vibes and weekend warriors all marching through and dancing along to the best music, sweetest melodies and smiliest faces all around :))))
So as a HUGE thank you to ALL of YOU who made it happen, we've decided to throw an off-the wall (DEFINITION style ;) FREE warehouse party this Bank Holiday Sunday at Victorian Vaults (opposite the Hoxton Hotel, Shoreditch) where we are going to celebrate 2011's summer and also have a fantastic time.
For this special DEFINITION we have decided to mix up things even more, inviting amazing new talent that's been making waves on the underground scene of the UK as well as new hot producers.
Our headliner Dean Tyler, has been working away behind the scenes of some key releases over the last year, from his remix of Jamie Jones' Summertime anthem to his recent collaboration with Hot Natured's Richy Ahmed as Volante on Alexis Raphael's Kitchens & Bedroom record which saw him hit number 3 in Beatport's Nu Disco chart (and currently still in the top 5). As a regular at roadblock event Creche and Afterlove, we're very glad to have him on board for our special Bank holiday event. Stay tuned as you'll be hearing Dean's name frequently from now on!!!
Joining him will be Digital Thrills, a duo that impressed at a previous warehouse event and left us with no choice but invite to come play at our party. This guys do Tech House/Deep and Nu Disco the way very few know how to present in the London and around the world ;)
DEFINITION is a party originating from our desire to demonstrate the quality and depth presently existent in a variety of electronic music genres and sub-categories. Focusing on the brilliance and excellence of tech and deep house but always finding time and space for introducing new and pioneering sounds.
Supporting and complementing our guests will be resident Montana Cruz and Jon Sojo after an impressive set for OneMore with Christian Burkhardt. They have new, freshly out of the bag, tracks that will be sending you to Sunday evening heaven, be ready !!!
DEFINITION is a warm atmosphere party consisting of a friendly-attitude crowd. Everyone invited and allowed-in is selected, we prefer crowd quality not crowd quantity. we would like to THANK YOU for bringing your best vibe and slickest dancing to our parties...words can not describe how much we APPRECIATE and LOVE YOU, the DEFINITION faithful!!
For guestlist requests please send FULL NAMES to firstname.lastname@example.com with subject FREE WAREHOUSE and we will confirm accordingly///
Sunday will be SPECIAL and we're glad to be spending it with you :)
See you soon,
Comments loading
Popular events
Fri, 30 Jun 2017
354 attending
Aarrival presents: Sonny Fodera, Mat.Joe & Marc Spence
Fri, 30 Jun 2017
348 attending
FABRICLIVE: Hospital Records x Jungle Jam
Sat, 1 Jul 2017
386 attending
EGG presents: Boris Brejcha (3 Hours set), Deniz Bul, Kyle E
Sat, 1 Jul 2017
362 attending
Art Night X Boiler Room
Sun, 2 Jul 2017
424 attending
ABODE Summer Sessions - July Garden Party
Fri, 7 Jul 2017
342 attending
EGG presents: Detlef B2B Latmun, Mihalis Safras & More
Sat, 15 Jul 2017
322 attending
Berlin Berlin - The Birthday: Sisyphos, Steyoyoke, Rumpelkammer, Homostash, Kuntskammers
Fri, 21 Jul 2017
409 attending
Garage Special with Matt Jam Lamont, Shola Ama (Live) & Marky B


text:
I remember that morning we were running late because someone slept in.
It was one of those Saturdays I enjoy so much, spending time with George, getting out
to all the yard sales and finding other ppls crap I can make into something wonderful.
I still had to make a delivery and an install before we could make any stops.
Heading down the road I spotted this cabinet and my heart started to pound in my chest.
Unsure if it was the A&W I just ate or the treasure I just saw it was time to get my hands back
on the wheel or get a ticket for not using a hands free device while driving.
George has an affinity for yard sales even when I am not there so he dropped
me off and back he went. George is a mighty force to be reckoned with but when it comes to dirt,
he's a goner.
Poor George picked up this filthy, spider ridden cabinet for me.
He was a one man crew picking up and loading this piece into my truck.
Once he returned you had to see his face, his dirty white shirt, and hear the story only he could tell,
With a piece that was this grimmy
I had to clean, sand, paint and wax the interior shelves,
lined all the drawers as well as the inside back panels.
There was some damage to the bottom edges so I cut that right off and added new legs.
Mixed up a lovely antique cream color of paint, added some iron scroll work to trick your eye
away from the building block shape, and installed a mirror into the door.
There are some new treasures to be found at
Sharing at:


text:
Preservation Hall Jazz Band marks 50 years at fest
In this Saturday, May 5, 2012 photo, audience members "second-line" as the Preservation Hall Jazz Band performs at the New Orleans Jazz and Heritage Festival in New Orleans. For five decades, Preservation Hall has served up New Orleans jazz for music lovers the world over. The New Orleans Jazz and Heritage Festival, on its closing weekend, marked that achievement by showcasing the world-renowned Preservation Hall Jazz Band in concert twice. (AP Photo/Gerald Herbert)
NEW ORLEANS (AP) [auth] — The Preservation Hall Jazz Band on Sunday marked its 50th anniversary with a jam session at New Orleans' Jazz Fest featuring a number of old friends.
On Sunday, the band closed the festival's Gentilly Stage with friends including Allen Toussaint, Trombone Shorty, the Rebirth Brass Band and Jazz Fest founder George Wein.
In a rare performance, the 86-year-old Wein introduced the band then played piano on their opening number — "Basin Street Blues." He got a rousing ovation after the song ended and he left the stage.
All of the performers then joined the band on stage for the finale.
The French Quarter-based music venue was founded in 1961 to protect and honor New Orleans jazz.
The New Orleans Jazz and Heritage Festival, on its closing weekend, marked the anniversary by showcasing the world-renowned band in concert twice.
Hall director Ben Jaffe, the son of founders Allan and Sandra Jaffe, said his parents' goal was to make sure New Orleans' unique musical traditions would be kept alive.
"Fifty years later, we're not too much different," Jaffe said. "The faces may be a bit different, but the experience is not tremendously different."
"It's important that the Preservation Hall people keep playing," added Wein, who also founded the Newport Jazz Festival in Rhode Island. "Times have changed, but the spirit and feel remain the same."
The Preservation Hall Jazz Band's members include 79-year-old clarinetist Charlie Gabriel, a fourth-generation musician; trombonist Frank Desmond, also 79; pianist Rickie Monie, 60; drummer Joseph Lastie Jr., 53; saxophonist Clint Maedgen, 42, on tuba; Jaffe, 41; and trumpeter Mark Braud, 38.
"New Orleans music is passed on through its traditions, with younger players learning from older ones," Jaffe said. "That remains central to our mission and even today, 50 years later, it's a continuing goal to cast a spotlight on the old and young that's very much alive."
"I have so much respect for them as musicians and for what they do," said Toussaint, 74, who at one time was the band's opening act. "It's a wonderful thing for our city and the world."
On Saturday, the band performed in the Economy Hall tent, the festival's traditional jazz venue.
Jaffe has been criticized by some for moving the band away from its traditional roots into different musical genres through collaborations with artists such as rapper Mos Def and rockers Lenny Kravitz and My Morning Jacket.
"I think when you talk of change, it's a part of the evolution of our traditions," he said in an interview. "Keeping any cultural tradition alive must reflect the generation of today or it will die. We generally play almost the same set that we have been playing. What changes most is the audience more than anything else.
"I think it's important to find a way to pay respect to the past while still balancing and staying true to yourself. That's the only way you're going to leave your own imprint on the music."
Associated Press writer Mary Foster contributed to this report.
Related Posts
Leave a Reply
You must be logged in to post a comment.
« 'Avengers' smashes record: $200.3 million debut George Lindsey, known as TV's Goober Pyle, dies »


text:
Ah, yes. New technologies can be baffling. This hilarious clip is from a Norwegian TV show, 2001. I love that a few English words can be heard such as "text" and "whatever."
I've watched it a zillion times and I still giggle when he snaps the spine down on the desk over and over in frustrated despair. That shrug is hilarious in any language.
Thanks for sharing that. Too funny. I'm always behind everyone else when it comes to new technology and can see my kids making a similar video about me one day.
Previous post: Preserving Flowers with Borax
Next post: Raise your Google IQ in two easy keystrokes


text:
One day, a young mother and her toddler son came in to look at pianos. The mother told me that her son was three, but had started lessons and was very enthusiastic. Having seen many proud parents, and observing that the tiny child was indeed very young, I was a bit skeptical about his level of enthusiasm — until he started examining with great care all the vertical pianos on the floor.
The boy had a limited repertoire: one short piece that covered a single octave, which he methodically tried on each register of each piano. He was so diminutive that the keyboards were at his eye level. He was very systematic in walking down the rows of instruments, giving each one his attention, until he stopped in front of a vertical that I had recently prepped to go to a statewide teachers' convention. He tried the piano with his little piece, said a few things to his mother that I didn't understand, and nodded his approval. Lots of pointing made clear that he had made his selection.
Mom explained that they couldn't make a decision until Father was consulted. When she tried to leave, her young charge was dismayed. He wanted that piano, and was most determined to have it. Most of his pleas were nonverbal, and included vigorous "no" head shaking and petulant body language. When she insisted that it was time to go, he sobbed and planted himself there, wrapping his little arms around the leg of the piano, and leaned his head against it, cheek to piano cheek. Wow, I thought. That, in a nutshell, is why we are here.
I was reminded afresh of the passion that drives us to love these instruments, of our excitement on hearing and creating beautiful sounds, of our joy at being able to convey our thoughts in music. Here, that passion had been unabashedly expressed as pure emotion.
He got his piano.
Sally Phillips
Steinway Piano Galleries
Alpharetta, Georgia
If you'd like to make a comment on any of the stories, please visit our Piano-Buying Stories blog page.
The Surprising Thing About Pianos
A Three-Year-Old Reminds Me
Why We Love Pianos
If you are looking for ADS FOR PIANO-RELATED PRODUCTS AND SERVICES and don't see any here, please turn off the AD-BLOCKING FEATURE in your web browser, or list www.pianobuyer.com as an EXCEPTION.
Piano-Buying Stories are copyrighted by their respective authors. Unauthorized use and/or duplication of a story without the express written permission of that story's author is strictly prohibited. Excerpts and links may be used, provided that full and clear credit is given to the author with appropriate and specific direction to the original content.
Steinberg, G. (Perzina, Gebr.)


text:
Latest Weather Blog
Content Tagged As metro_council_
Tagged News
Metro Council seats will be decided in a runoff
November 9, 2016 12:28 AMBATON ROUGE - Runoff elections will be needed to determine final decisions in the East Baton Rouge Parish Metro Council election. Incumbents Chandler Loupe, Scott Wilson and Buddy Amoroso won enough votes to miss a runoff and were elected back to the council. Newcomer Matt Watson was elected to... more »
Metro Council kills smoking ban proposal Play Video
April 13, 2016 7:15 PMBATON ROUGE – The Baton Rouge Metro Council killed a proposal that would have banned smoking in all businesses, including bars and casinos. Smoking ban fails. pic.twitter.com/qiXjg0jauI — Brittany Weiss WBRZ (@MsBWeiss) April 13, 2016 The ordinance was designed to include a ban on... more »
With Metro Council approval, CATS to cut two routes in December
November 10, 2015 11:50 PMBATON ROUGE - Changes to CATS routes will start December 6th, and that will include the elimination of two following a vote by Metro Council Tuesday. The bus company asked city leaders to let them cut the under performing routes: O'Neal Park and Ride and the Downtown to Mall... more »
Body cameras are voted down by Metro Council
June 10, 2015 11:15 PMBATON ROUGE - The Metro Council voted down body cameras this evening. Members debated body cameras for almost an hour before the vote. The pitch was to require cops in Baton Rouge to wear body cameras. Although many of the council members agreed they wanted police to wear body... more »
Council approves plan to build sidewalks on deadly road
January 28, 2015 11:31 PMBATON ROUGE - Metro Council approved two items Wednesday dealing with sidewalk construction . The approvals allow DPW to move forward with accepting federal money to build sidewalks in Gardere and a neighborhood in North Baton Rouge. The city will used surplus money from similar projects to fund its... more »
With budget approval, council agrees to more police academies
December 9, 2014 9:32 PMBATON ROUGE- City leaders approved the mayor's $830 million spending plan for 2015 which will include two police academies to train as many as sixty new police officers. The budget was approved Tuesday evening. The Metro Council made no changes to the plan but did voice some concern about... more »
Metro Council approves mall, hospital annexations Play Video
May 14, 2014 4:33 PMBATON ROUGE - Metro Council members chose to approve petitions to annex property including the Mall of Louisiana and Baton Rouge General Hospital into the city limits of Baton Rouge. The vote was 9-3 in favor of annexation; council members Heck, Amoroso, and Loupe voted against. A motion by... more »
St. George supporters ask for "no" vote on annexation Play Video
May 9, 2014 7:10 PMBATON ROUGE- St. George organizers ask people to take a stand by asking the Metro Council to vote no to annexation. The Baton Rouge Metro Council is meeting on Wednesday, May 14, to vote on the annexation of the Mall of Louisiana and the Baton Rouge Medical Center. St.... more »
Bridge renaming for Trevor Sims approved
November 13, 2013 6:06 PMBATON ROUGE - Metro Council members approved a measure Wednesday night to rename an East Baton Rouge Parish bridge in honor of Trevor Sims, a terminally-ill boy who fought hunger in the parish before his death. The council voted unanimously to rename the North Boulevard Bridge the Trevor Sims... more »
Settlement approved in hair-pulling incident
June 26, 2013 10:33 PMBATON ROUGE- City leaders approved a $30,000 settlement after a woman's hair was pulled by a Baton Rouge Police Officer two years ago. Melinda Morris said Sgt. Bobby Schilling used excessive force when he drug her into a ditch while working a traffic crash. Schilling and city police... more »
City leaders to decide on $30k settlement in hair-pulling case
June 24, 2013 10:14 PMBATON ROUGE- City leaders are set to decide Wednesday on a $30,000 settlement for a woman suing the city over an incident involving a police officer. Melinda Morris has said an officer pulled her by her hair and forced her to fall into a ditch in September 2011. Morris... more »
City leaders in BR to talk about vehicle take-home policy
March 26, 2013 10:22 PMBATON ROUGE- City leaders could begin discussing making changes to the take-home vehicle policy Wednesday. Metro Councilwoman C. Denise Marcelle is proposing making changes to the policy, including removing the 24 mile exception and limiting personal usage of city-owned vehicles. "We pay for the fuel and I think... more »
Council starts discussion on electing BR police chief Play Video
February 27, 2013 10:16 PMBATON ROUGE- City leaders tabled discussion about electing the police chief during Wednesday night's Metro Council meeting. But, there was no shortage of opinions from citizens who voiced their concerns. "Let me say that Baton Rouge can no longer accept an appointed police chief. And, we can no... more »
Buyout deal reached
January 23, 2013 11:29 PMBATON ROUGE- City leaders approved a buyout plan for a neighborhood on the northern end of the city near a wastewater treatment plant. People who live in University Heights have been asking for a buyout for years. They complain the treatment plant stinks and has ruined their quality of... more »
Metro Council to talk about neighborhood buyout again
January 8, 2013 10:10 PMBATON ROUGE- City leaders will discuss a possible buyout for people who live near a wastewater plant in the northern part of Baton Rouge Wednesday. People in University Heights, a neighborhood near Scenic Highway, have said the plant stinks and has ruined their quality of life. The Baton... more »


text:
All The King's Men (1949)
All the King's Men (1949) is the fictionalized account of the rise and fall of a backwoods rebel - a story inspired by the rule (and abuse of power) of Louisiana's colorful state governor (1928-32) and Democratic U.S. Senator (1932-35), notorious Huey Pierce Long - "The Kingfish." It is a melodramatic story of the corruption of power by an ambitious demagogue, adapted and based on the Pulitzer Prize-winning and best-selling 1946 novel of the same name by Robert Penn Warren, and filmed from a script by producer/screenwriter/director Robert Rossen (known for directing other films such as Body and Soul (1947) and The Hustler (1961)).
The main difference between the novel and the film is the reversal of the major roles: the narrating newspaper reporter takes precedence over the power-hungry governor in the novel. In the film, the secondary character is the reporter, while the central character is lawyer-turned-politician Willie Stark. One of the film's posters proclaimed: "He thought he had the world by the tail...till it exploded in his face...with a bullet attached..."
The great political film was a breakthrough film for Broderick Crawford from his B picture status - his performance is very compelling and impressive as he is transformed from a backwoods, honest and naive lawyer into a dirty, unscrupulous and sleazy politician. Of the film's seven Academy Awards nominations, it won three major honors: Crawford won the Best Actor statuette, Rossen (as producer) won the Best Picture Oscar, and Best Supporting Actress went to Mercedes McCambridge (in her screen debut). Its other nominations were: Best Supporting Actor (John Ireland), Best Director and Best Screenplay (both for Rossen), and Best Film Editing.
Writer/director Steven Zaillian remade the film 57 years later, as All the King's Men (2006), with mixed reviews. It starred Sean Penn as Willie Stark (Broderick's role), Jude Law as Jack Burden (Ireland's role), Kate Winslet as Anne Stanton (Joanne Dru's role), Mark Ruffalo as Adam Stanton (Shepperd Strudwick's role), and Patricia Clarkson as Sadie Burke (McCambridge's role). Reportedly, Zaillian never saw the original film, and adapted the screenplay solely from Robert Penn Warren's novel.The Story
Under the film's credits, crowds of fanatical political supporters from the countryside congregate in the capital city to support their candidate, displaying placards and banners that read: WILLIE'S LAW IS OUR LAW, and WIN WITH WILLIE. Torchlight flames are superimposed on the brawny image of the egomaniacal political figure of Willie Stark, who preaches his powerful message to them. [These scenes are excerpted from the final sequence in the film.]
Everything is told through the eyes of an admiring young Chronicle newspaperman, Jack Burden (John Ireland), who is sent to report on a Southern politician named Willie Stark (Broderick Crawford) - said to reportedly be "an honest man" by Burden's editor. [Burden's story is, in actuality, the truth of the rise and fall of a political king, who like Humpty Dumpty in the rhyme, falls from grace and can't be put back together again.] The reporter locates Stark in small Kanoma City (in Kanoma County) in an unnamed state - "a typical hot, dusty, backwoods county seat," speaking to a group of citizens and rallying for them to vote for him as County Treasurer. At first in the late 1920s during his campaign, poor, honest, and idealistic Stark is for reform, justice, the underprivileged, and the underdog as he rails against the local County Commissioners:
Why have they used every dirty method known to make sure I'm not elected County Treasurer? Well, I'll tell ya why - because they're afraid of the truth, and the truth is this. They're trying to steal your money. Yeah, I said steal. The County Commissioners rejected the low bid on the schoolhouse. Why? Well, they'll tell ya the reason is the job will be done better. The County Commissioners would have you believe that they're interested in public welfare. They're interested in welfare, sure, but it's their own.
Stark is pressured to break up his assembly due to an ordinance selectively enforced by the local authorities to deliberately disrupt the proceedings and prevent the passing out of handbills, but after a quick arrest due to his resistance, he is released by fat city boss Mr. Tiny Duffy (Ralph Dumke). Burden drives out with Stark to his rural home, where he meets Willie's long-suffering schoolteacher-wife (of nine years) Lucy (Anne Seymour), his father (H. C. Miller), and their sullen fifteen year-old step-son Tom (John Derek). While Willie aggressively eats his chicken dinner with a single-minded appetite, he is determined to run and stay in the race ("get(s) the truth to the people" according to his wife), no matter how much humiliation and harrassment he receives. With folksy charm, he vows: "I'm gonna run. They're not gonna kick me around like I was dirt." When adopted son Tom returns home, he describes how Duffy's goons were waiting for him, and threw away his stepfather's handbills. Willie again swears: "I'm gonna run even if I don't get a single vote."
With conviction, Burden types his news article about his impressions of Stark - WILLIE STARK, AN HONEST MAN:
In a state ridden for years by corrupt politics, the appearance on the scene of a man who is a politician and yet dares to oppose the political machine is indeed a rare phenomenon. Willie Stark is such a man. He is as much a part of the back country as the very sun-scorched hills. He truly represents the people. What man (sic) turn out to be the sad commentary is that the people will not be aware of it. It has been a long time since this locale has witnessed the appearance of a man with the sincere and forthright manner of Willie Stark. Coming from farmer stock, he has retained all of....
One gets a very definite impression that Willie Stark will not bow to the past, but will make a new future. It might conceivably happen that in his future, also lies the welfare of the people of this State. "I'm gonna run," he shouted, "and you're not gonna stop me. I'm gonna run even if I don't get a single vote."
As I watched him shake his big fist and listened to his words boom out across that field, I had the feeling that here was a man with a will of iron. I had the feeling that Willie Stark would neither be steered away nor scared away from his purpose. I had the feeling that in Willie Stark, Kanoma County had found that rare thing: an honest man with courage.
After producing the sympathetic article that befriends Stark, Burden takes a well-deserved two or three-week vacation in his childhood home community of Burden's Landing (named for his ancestors), about 130 miles from Kanoma City. In Burden's voice-over as he crosses over to the small, peaceful and isolated island town, he muses: "It was separated from the mainland by a body of water. For the first time, I wondered if it wasn't separated by more than that." Jack greets his Southern society mother (Katherine Warren), but is coldly distant from his stepfather Floyd McEvoy (Grandon Rhodes). A respected and distinguished inhabitant of the island community is patriarchal Judge Montgomery (Monty) Stanton (Raymond Greenleaf). Stanton's socialite niece is Anne Stanton (Joanne Dru) [mis-spelled as Ann in a newspaper article], Burden's girlfriend, and Stanton's nephew (brother to Ann) is noted physician Dr. Adam Stanton (Shepperd Strudwick) - a close childhood friend of Jack's.
At one of many fancy social occasions, discussion flares over the direction of Jack's troubled career choice and conflicting political viewpoints regarding Willie Stark. Later that evening, while standing under an imposing portrait of Anne's father (the late former governor) in the darkness, Jack is unclear regarding marriage to Anne because of his own indecisiveness, his ambivalence about his family, and his aim to prove himself to his aristocratic background:
Jack: Anne, Burden's Landing is a place on the moon. It isn't real. It doesn't exist. It's me, pretending I live on what I earn. It's my mother, trying to keep herself young and drinking herself old doing it. She and Adam living in this house as though your father was still alive. It's an old man like the judge, dreaming of the past. Anne, come away with me.
Anne: And do what?
Jack: Live in a shack and eat red beans. Anne, what do you want me to do?
Anne: Oh Jack, you haven't been sure. You've gone from one thing to the other. A year at law school and now this job as a reporter.
Jack: Are you afraid I can't make a living?
Anne: Oh no Jack, it isn't that. I don't care about the money. It's just that I, I want you to be something.
Jack: What is it you want me to be?
Anne: I don't know. It's just that I want you to be, to do something important.
Jack (sarcastically turning toward the portrait): Like your father? All right, I'll run for governor. Anne, I'm sorry, I'm sorry I said that.
Anne: All right, Jack. I'll go away with you. I'll do anything you want me to. (They kiss.)
Jack: I've wanted you to say that more than anything in the world. And now that you have said it - Anne, I guess you were right. I'm not sure of anything, including myself. I'm not sure I could live up to the...Anne, wait for me. Please wait for me.
Anne: I'll wait for you.
Burden cuts his vacation short and returns to the newspaper. Although honorable and truthful and considered a "log cabin Abe Lincoln," Willie's efforts are hopeless against the state's "dishonest" political machine, and he loses the County Treasurer election ("I guess that's the end of Willie Stark," Burden mutters). After the loss, Willie studies law at home under the patient tutelage of his educated wife to become a hick lawyer. In a montage - Willie proudly hangs up his framed Bachelor of Law diploma from Kenport Law School, accepts indigent cases in his new law practice, and works long hours behind his storefront window (decorated with bold letters WILLIE STARK, ATTORNEY AT LAW).
Soon, Willie's luck turns when his prophetic campaign warnings concerning graft in a Kanoma City Grammar School building contract come true. The poorly-constructed school fire escape breaks loose from a brick wall support during a routine fire drill, collapses, and kills several children. After the funeral service, Willie is remembered as "an honest man" by some of the town's victimized citizens - "If we'd only listened to you, Willie." A Kanoma City news editorial titled "Voice in the Wilderness" praises Willie's foresight:
Kanoma's recent school tragedy serves as a potent reminder that a man named Willie Stark, a citizen of Kanoma City and defeated candidate for County Treasurer, fought bitterly against the awarding of our schoolhouse contract. His was "the voice in the wilderness" that fell on deaf ears, and the children of Kanoma City paid the price. Nothing can ease the pain in the brave hearts of our bereaved parents who suffered their loss bravely.
Willie seeks damages on the victims' behalf, and thus gains statewide notice for his successful prosecution of the unscrupulous grafters. Stark pastes headlines from newspapers into his scrapbook. They read:
SCHOOL VICTIMS SUE COUNTY; STARK FILES DAMAGE SUIT - 'Will Prove Graft Cause of Tragedy' Says Atty. Stark.
CITIZEN'S COMMITTEE FORMED! Draft Stark to Lead Fight to Rid State of Graft
CITIZENS' COMMITTEE DEMANDS STATE-WIDE INVESTIGATION - Stark Leads Fight - "The People's Voice Must Be Heard," Says Willie
RURAL AREAS IN POLITICAL REVOLT - STATE MACHINE THREATENED - "Sweep Them Out of Office" Cries Willie Stark in Impassioned Speech
Burden is again assigned by his news editor to "stay with him" and follow Stark's progress in taking "the hick vote" away from the political machine. Willie is encouraged to run for state governor by state bosses in the capital, but he is really being manipulated by them to split the rural "hick" vote. (Three candidates are on the ballot: the machine candidate Joe Harrison, McMurphy, and Willie Stark.) During whistle-stop campaigning for the position, Willie speaks to the people about a "balanced tax program," but turns off potential voters by his wooden and leaden style and reliance on facts and statistics. When Burden realizes that the gullible Willie is being framed by the political machine for their own purposes, he confers with Sadie Burke (Mercedes McCambridge) - a cool, conniving and calculating political aide:
Jack: You don't have to be smart to frame a guy like Willie Stark.
Sadie: No, no brother, you don't.
Jack: It is a frame, isn't it?...To split the vote and win the election for Harrison, huh?
Sadie: You know, why do ya ask?
Jack: I just want to make sure. Look, why don't you tell the boys to save their money? Willie couldn't steal a vote from Abe Lincoln in the cradle of the Confederacy.
Sadie: I wish the poor --- had enough sense to have somebody give him a good reason for the beating he's going to get. 'Cause this way, all he gets out of it is the ride. Hey, those speeches, ain't they awful? Ain't they just plain awful? Question to you: If somebody told him he was a sucker, do you think he'd quit?
Jack: I don't know, Sadie. I really don't know.
But Burden is reluctant to reveal the truth to the dim-witted Willie about how he will soon be defeated in the state's gubernatorial race by the machine. Yet he does advise Willie to stir up the indifferent voters:
Just tell 'em you're gonna soak the fat boys and forget the rest of the tax stuff...Willie, make 'em cry, make 'em laugh, make 'em mad, even mad at you. Stir them up and they'll love it and come back for more, but, for heaven's sakes, don't try to improve their minds.
Willie senses that Burden is right, and that he won't be governor: "A man don't have to be governor...I'm gonna lose, Mr. Burden, I know that. Don't try and fool me...I would have made a good governor, better than those other fellas."
When Sadie joins their conversation and inadvertently (and callously) admits that Willie was framed by the machine, her revelation is stunning:
...you've been framed, you poor sap...Oh you decoy, you wooden-head decoy, and you let 'em. You know what you are? Well, you're the goat. You are the sacrificial goat. You are a sap because you let 'em...They didn't have to pay a sap like you. Oh no. You were so full of yourself and hot air. All you wanted was a chance to stand up on your hind legs and make a speech. 'My Friends. My Friends, What This State Needs is a Good Five-Cent Cigar. What this State Needs Is A...'
After learning that he was used, Willie promptly proceeds to get drunk from a bottle of bourbon and then passes out. The next morning, still recovering from a hangover and half-conscious, he is led to the Upton Fairgrounds for a campaign barbecue and speech. A ferris wheel spinning in the background reflects the spinning within Willie's head. To recover before he is due to give his canned talk, Willie sits in a playground swing where other children are playing, and drinks down coffee (and a generous amount of bourbon).
When he finally ascends the campaign platform, and lurches onto the stage, Sadie and Burden both wonder if Willie is capable of speaking to the crowd:
Sadie: How'd you get him here? He was out stiff.
Jack: The hair of the dog that bit him.
Sadie: Hair? He must have swallowed the dog.
To their surprise, Willie develops his own fighting style and delivers a rousing, memorable speech - after tossing away his prepared speech. A cut-away to them in the middle of the speech displays their astonishment. Stark's bull-headed oratorical talent, loosened by the booze, truly speaks to the people. [This speech single-handedly won the Academy Award for Broderick Crawford.] He identifies himself with the hick audience that is awestruck by his words. The speech ends with a montage of a closeup of his ranting face delivering powerful and thundering words, superimposed over flames:
My friends. I have a speech here. It's a speech about what this state needs. There's no need in my telling you what this state needs. You are the state and you know what you need. You over there, look at your pants. Have they got holes in the knees? Listen to your stomach. Did you ever hear it rumble for hunger? And you, what about your crops? Did they ever rot in the field because the road was so bad you couldn't get 'em to market? And you, what about your kids? Are they growing up ignorant as dirt, ignorant as you 'cause there's no school for 'em? No, I'm not gonna read you any speech. (He casts his speech away behind him.) But I am gonna tell you a story. It's a funny story so get ready to laugh....Get ready to bust your sides laughin', 'cause it's sure a funny story. It's about a hick. A hick like you, if you please. Yeah, like you. He grew up on the dirt roads and the gully washes of a farm. He knew what it was to get up before dawn and get feed and slop and milk before breakfast, and then set out before sunup and walk six miles to a one-room, slab-sided schoolhouse. Aw, this hick knew what it was to be a hick, all right. He figured if he was gonna get anything done, well, he had to do it himself. So he sat up nights and studied books. He studied law, because he thought he might be able to change things some - for himself and for folks like him. Now I'm not gonna lie to ya. He didn't start off thinkin' about the hicks and all the wonderful things he was gonna do for 'em. Naw, naw, he's done it all thinkin' of number one. But something came to him on the way. How he could do nothin' for himself without the help of the people. That's what came to him. And it also came to him with the powerful force of God's own lightning back in his own county when the school building collapsed 'cause it was built of politics' rotten brick. It killed and mangled a dozen kids. But you know that story. The people were his friends because he'd fought that rotten brick. And some of the politicians down in the city, they knew that, so they rode up to his house in a big, fine, shiny car and said as how they wanted him to run for governor...And he swallowed it. He looked in his heart and he thought, in all humility, how he'd like to try and change things. He was just a country boy who thought that even the plainest, poorest man can be governor if his fellow citizens find that he's got the stuff for the job. All those fellows in the striped pants, they saw that hick and they took him in...Now, listen to me, you hicks. Yeah, you're hicks too, and they fooled you a thousand times, just like they fooled me. But this time, I'm gonna fool somebody. I'm gonna stay in this race. I'm on my own and I'm out for blood. Now listen to me, you hicks! Listen to me, and lift up your eyes and look at God's blessed and unfly-blown truth. And this is the truth. You're a hick, and nobody ever helped a hick but a hick himself!...I'm the hick they were gonna use to split the hick vote. Well, I'm standin' here now on my hind legs. Even a dog can learn to do that. Are you standin' on your hind legs? Have you learned to do that much yet?
His campaign literally catches fire. A headline from the newspaper: "STARK CHANGES BOOMING!" reflects the strong resurgence of voter interest in Willie as a viable candidate in rural areas. Fearful of his growing power and that Stark "is getting too big for his britches," city bosses order strong-armed retaliation and bribery against the leader of the hicks. When Burden's editor demands that the Chronicle support Stark's opponent Harrison, the politico's man, the principled reporter quits his job.
Although Stark loses the election to Harrison in a close race, Stark sweeps rural areas. The city vote decides the election, and a record number of votes are polled. Confident that he has touched a nerve with the people and will win the gubernatorial race the next time around ("I learned something...how to win"), Willie is joined at a bar for drinks by Sadie, Burden, and stuttering henchman Sugar Boy (Walter Burke) - a bodyguard and assistant who previously worked for his opponent Tiny Duffy.
Four years later, Willie conducts a second campaign. Meanwhile, Jack "drifted from job to job" and grew "further and further away from Anne and the life at Burden's Landing." According to Jack's voice-over during another long montage of Willie's attacks against the corrupt 'old machine' and his slogans of support for the people:
But Willie wasn't drifting. He knew where he was going. He had his foot in the door and he kept right on pushing to get in. He had lost the election, but he had won the state - and he knew it, and the people knew it. They were all hopping on his bandwagon, even Tiny Duffy. Yup, Willie came back like he said he would...
He won the hearts of his constituents by fighting state government corruption and proclaiming that his new platform was to "soak the fat boys and I'm gonna spread it out thin." However, Burden slowly becomes disillusioned and troubled as he observes Stark's corruptible rise to power ("strange deals" with special interests) the next time around:
Willie was right. He'd learned how to win. He spent a lot of money doing it. An awful lot of money. I was beginning to wonder where he got it from. There were rumors throughout the state that Willie was making deals with all kinds of people, strange deals...The second time out, it wasn't a campaign, it was a slaughter. It was Saturday night in a mining town. Yup, Willie came back like he said he would. He came back and he took me with him.
Burden (a "college man") joins Stark's entourage and team as chief aide, hatchet man and speech writer. Surprisingly, Willie surrounds himself with his previous opponents, including Tiny Duffy. He also hires shrewd Sadie Burke as his secretary and campaign manager, after she switches allegiances from the political machine. Willie confidently brags about his disregard for financial resources and his wheeling-dealing: "Money, I don't need money. People give me things...because they believe in me."
Willie is brought by Jack to Burden's Landing to seek support from Judge Stanton and other guests during a reception in the Stanton estate. During a question and answer period, liberal Dr. Stanton asks about alleged "deals" being made by Stark with the very groups he supposedly opposes. Stark admits the fact: "I have nothing to hide - I'll make a deal with the devil if it will help me carry out my program. But believe me, there are no strings attached to those deals." He then responds with a question of his own concerning where good comes from. He answers his own question - stipulating that the end justifies the means:
Do you know what good comes out of?...Out of bad. That's what good comes out of. Because you can't make it out of anything else. You didn't know that, did you?
He believes that any worthwhile programs (such as schools, highways, hospitals) require some degree of corruption, deal-making and dirty power politics. He solicits the community's support: "There's a time to talk and there's a time to act. I think the time to act is right now, and with your support, I not only will win, but I will do all of the things I promise. I need your help - oh, I need it badly, but I'm not gonna beg for it. In the name of this state, which we love, in the name of the governor in whose house we meet, I demand it." Afterwards, when he has been reassured of their support, he craftily promises that the Judge will be made his Attorney General. Anne displays her admiration for the dynamic candidate.
Stark is elected governor - a "smashing victory throughout state," according to the newspapers. Cries resound in the street and call for Willie to make a victory speech to the cheering crowds: "WE WANT WILLIE!" Beneath a large poster of himself, he vows to keep his populist campaign promises:
I'm going to build a hospital, the biggest that money can buy, and it will belong to you. Any man, woman, or child who is sick or in pain can go through those doors and know that everything will be done for them that man can do. To heal sickness, to ease pain, free - not as a charity but as a right. And it is your right, do you hear me? It is your right. And it is your right that every child should have a complete education. That any man that produces anything can take it to market without paying toll, and no poor man's land or farm can be taxed or taken away from him. And it is the right of the people that they shall not be deprived of hope.


text:
I have it: 10 I want it: 22
Designers » F-H » Hermes Chypre Floral « Groups
I have it: 10 I want it: 22
24 Faubourg Eau de Parfum Edition Numero 24 by Hermes is a Chypre Floral fragrance for women. This is a new fragrance. 24 Faubourg Eau de Parfum Edition Numero 24 was launched in 2015. Top notes are orange, peach, hyacinth, ylang-ylang and bergamot; middle notes are black elder, iris, orange blossom, gardenia and jasmine; base notes are sandalwood, amber, patchouli and vanilla.
Perfume rating: 3.82 out of 5 with 17 votes.
Top Notes
Middle Notes
Base Notes
drag slider to vote
|long lasting
|very long lasting
drag slider to vote
This the same fragrance at the original. This also is like a sister to older formulations of Chanel Coco edp and parfum.
Could anyone tell me if there is any difference to the original 24 Faubourgh EDP?
It confuses me that they both list exactly the same notes, but the original is not retracted from stores. The bottle is slightly different, but otherwise i'm not able to detect the difference.
I'm a newbie in the universe of parfumery, and even more so when it comes to chypres.
However i really like this perfume - and the original obviously, since they smell more or less the same to my rather unexperienced and untrained nose.
Anyone, please assist this humble though eager-to-learn parfumista!
Eternal gratitude!!
At first this reminded me of Chanel 19 though 24's opening is more balanced and not as loud as 19 needs time to settle. On my skin the iris in both is very prominant. I get big floral with mild wood and still milder citrus in the main stage. I find both powdery, which I'm not usually a fan of, but I do like it in both of these; however, the 19 has the green difference also. I'll need to experiment more with this. I like this Hermes, but I find i like my Fan di Fendi more. Not that they're anything a like, just both pretty gold bottles : ) 24 definitely has wonderful longevity and sillage. If you like powdery florals, I think you'd enjoy this, but if your really looking for floral citrus, you may want to sniff elsewhere. I find the Orange intensive only in the first stage, not much citrus other than that or following. Of course, that may just be me. I'm curious to smell this on someone else's skin. I also think this could be unisex easily. Nice : )
Become a member of this online perfume community and you will be able to add your own reviews.
Sponsored offers:
This page contains information, reviews, perfume notes, pictures, new ads, vintage posters and videos about Hermes 24 Faubourg Eau de Parfum Edition Numero 24 fragrance but we do not warrant the accuracy of information. Trademarks and logos belong to respected companies and manufacturers and are used solely to identify products and companies. If you have more information about Hermes 24 Faubourg Eau de Parfum Edition Numero 24, you can expand it by adding a personal perfume review. Fragrantica has a unique user-driven classification system and you may classify 24 Faubourg Eau de Parfum Edition Numero 24 by Hermes. Click on the appropriate options on the fragrance classification form below the perfume picture. Also, you can find links to 3rd party websites/Internet stores, but Fragrantica has no access or control over those websites. We do not make guarantees nor accept responsibility for what you might find as a result of these links, or for any future consequences including but not limited to money loss. User reviews of 24 Faubourg Eau de Parfum Edition Numero 24 by Hermes represent the views of the credited authors alone and do not reflect Fragrantica's views.
Popular brands and perfumes:


text:
Alright Id like to get some type of front lip spoiler diffusser for my car. I dont want anything like the votex kit or an actual body kit, just something that attaches to the existing underskirt/bumper.
Any info or ideas or where I could find something like this would be great!!


text:
Stunning and simple all the way when it comes to this scandinavian inspired dining table.
This is a quality piece of furniture made from a Solid American Oak and has a gorgeous natural finish. Measuring at a nice 160cm long it is the best solution for any dining space or corporate sitting environment.
Our Geo dining table is a right on trend piece and will suit any contemporary urban environment.
- Made from Solid American Oak,
- Single carved legs in a contemporary 'geo' design,
- Natural finish,
- Dimensions : 160cm length x 80cm width x 75cm height,
- Colour : Natural Oak as advertised,
- Commercial grade quality
Shipping & Warranty
To find out the shipping cost online, simply enter your postcode into the 'Shipping Calculator' located to the left of the screen and receive an estimated shipping cost direct to your door. This shipping calculator prices is for Courier Shipping which offers kerb side or front doolr delivery. In some cases of larger or oversize items it will be required for us to send out with a Furniture Removalist which can result in higher shipping cost as it's a personal hand load/unload service to you.
In-stock furniture will generally be dispatched from our Gold Coast warehouse within 24 - 48 business hours from receipt of payment.
Delivery time for out of stock items can be delayed due to changes in shipping schedules, dock strikes, container x-rays, carrier delays and overseas suppliers changing dates. Dates are estimates only and we apologise in advance for any delays that may be experienced.
If you have any questions regarding delivery or shipping feel free to contact us at firstname.lastname@example.com
Live in QLD? Then save on shipping with our Free Pickup option from our Gold Coast Showroom.
Come and see, touch and feel our high quality products yourself and take them home with you the same day. No waiting time needed and we try and display as many products as our showroom has room for.
FURNITURE FETISHPremium Quality Replica Designer Furniture....Lowest Possible Prices!
You may also be interested in the following product(s)
Urban Geo Table & 4 x Macey Dining Chair - Natural Package
Package Price: $1,199.00


text:
20GHz Dual Channel Electrical Module
Manufacturer's part number :
Description :
The Keysight Technologies 86112A provides two accurate measurement channels with user-selectable bandwidths of 12.4 or 20 GHz. The lower bandwidth mode provides excellent oscilloscope noise performance for accurate measurement of small signals. The high bandwidth mode provides high-fidelity display and measurement of very high-speed waveforms.
Technical Data :
Quote and availability
- Two electrical channels with 3.5 mm (m) connectors
- Bandwidth >= 20 GHz (standard unit), >= 30 GHz bandwidth (option HBW)
- Characteristic rms noise: 0.25 mV (12.4 GHz BW setting), 0.5 mV (20 or 30 GHz BW setting)
- Maximum input signal: ± 2 Vdc
- Electrical Inputs: 3.5 mm male, ships with factory-installed 3.5 mm (f-f) connector savers (5061-5311 or similar adapter)
- Compatibility: 6100A/B Infiniium DCA Mainframes, 86100C Infiniium DCA-J Mainframe 86100D Infiniium DCA-X Mainframe


text:
Ask Colt.
This is a discussion on Recommendation on Gun Cleaner for CEREKOTE within the Firearm Cleaning & Maintenance forums, part of the General Firearm Discussion category; I usually use Gun Scrubber Synthetic for my H&K and Walther which are both polymer based guns. The new Colt Defender of course has been ...
Ask Colt.
An armed society is a polite society. Manners are good when one may have to back up his acts with his life. - Robert A. Heinlein
I've used MPro7 for years on all finishes, including Cerakote. It works well, smells good, and doesn't irritate my skin.
Treat me good, I'll treat you better. Treat me bad, I'll treat you worse.