text:
sousa bed and breakfast-london
london bed and breakfast
location on the map
contact us
calendar for availability/prices/booking online
london bed and breakfast
calendar for availability/prices/booking online
contact us
location on the map
calendar for availability/prices/booking online
This Calendar is 100% updated everyday;
rooms will be hold 24 hours only if payment have not been made reservation will be cancel automatically.
If a guest's credit card is declined, the reservation will still be created. The guest can attempt a reservation with a different credit card or Paypal in order to book it.
banking online or bank transfer are welcome but fees for transaction if occur are guests own responsibility.
Please send me reservation first so you can proceed.
how to book the room(s);
iF YOU WISH TO BOOK MORE THAN ONE ROOM PLEASE SELECT BOTH 2 OR MORE ROOM,
After reservation form it will take you to the next page where it says
make paypal payment,
you must click the
buy now
button in order to pay as in order to book the room(s) minimum deposit applies.
option 1=pay with your paypal account.
option 2=don't have paypal account click link
new with paypal
and pay as paypal guest with
credit or debit card
inquires are checking every 1 hour by contact us form or email.
Booking information
bookings are from 2 nights only for winter times
Normally, we require a two night minimum stay over weekends - however we can sometimes accommodate a one night stay with £5.00 supplement per person.
Unfortunately No Pets allowed
Check in from 8:00 pm or by prior arrangement .
Check out 10.00 am on day of departure.
If you want to leave the luggage after 10:00am on your departure day on the premises (not in the room) £5.00 will be charge alternatively there are lockers in most stations to leave your luggage.
Luggage drop in the morning option by prior arrangement (not in the room as it needs to be clean from previous guest's)
It is our policy that we do not accept arrivals later than Midnight.
same day booking arrivals our policy is that we do not accept arrivals earlier than 8pm and later than 10:00pm.
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How to Buy
Solutions Overview
This script is Copyright (C) 2006-2015 Tenable Network Security, Inc.
Synopsis :
The remote Gentoo host is missing one or more security-related
Description :
The remote host is affected by the vulnerability described in GLSA-200603-11
(Freeciv: Denial of Service)
Luigi Auriemma discovered that Freeciv could be tricked into the
allocation of enormous chunks of memory when trying to uncompress
malformed data packages, possibly leading to an out of memory condition
which causes Freeciv to crash or freeze.
Impact :
A remote attacker could exploit this issue to cause a Denial of
Service by sending specially crafted data packages to the Freeciv game
Workaround :
Play solo games or restrict your multiplayer games to trusted
See also :
Solution :
All Freeciv users should upgrade to the latest version:
# emerge --sync
# emerge --ask --oneshot --verbose '>=games-strategy/freeciv-2.0.8'
Risk factor :
Medium / CVSS Base Score : 5.0
Family: Gentoo Local Security Checks
Nessus Plugin ID: 21084 (gentoo_GLSA-200603-11.nasl)
Bugtraq ID:
Get Nessus Professional to scan unlimited IPs, run compliance checks & more
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subset: commoncrawl


text:
|2,583 sq. ft.
|Property type
|Minimum Stay
|7 nights
Luxury villa with large swimming pool 12 m x 6 m, with removable security barrier and alarm. Extensive terrace with very big new gas barbecue for outside dining, offering views of the surrounding countryside and coastline. Beautifully landscaped gardens with fruit and olive trees and ample parking. Big spacious light rooms . Well equipped kitchen with two fridge freezers and all the usual appliances for dealing with large groups. New flat screen TV, with two satellites so numerous channels including BBC , fast broadband internet access, iPhone speaker dock.
Two English speaking guardians or caretakers live In a separate apartment on the property on opposite side from the villa and pool and garden . They are on hand to greet you and answer any questions if needed
We are an Australian family who have enjoyed, for some years, wonderful holidays with family and friends as tenants at Villa Gezanne.
Gezanne came up for sale and we were fortunate to become the new owners. Much to our delight, the previous owners - Marian and her daughter Briane, who had welcomed us so warmly to the property for many years, agreed to continue to greet guests to Gezanne and look after the property. Their local knowledge, kindness and enthusiasm has been wonderful for us and other guests over the years. Marian and Briane live on site in a separate part of the property in the guardian's flat. We were drawn to Gezanne because of the house's character, spacious garden, sizeable pool, pleasant views and its great location - in a quiet secluded area but just a not far from the beach, the village of Biot and Antibes. Gezanne is a great base to explore the Riviera and the Alpes Maritimes and each year we find more and more interesting places to see and things to do."
The rooms are very spacious and light. The house has a welcoming feel to it. The pool is larger than normal for a private villa plus can been heated on request . The garden is large and secluded, with terraces of Olives , Citrus fruits which look stunning when light up at night. The surrounding area is quiet.
Check out our short video by on this site going to Overview, then right arrow to move through all the photos , the last photo if you wait acutally appears as a video
We had a fantastic 2 weeks at Villa Gezanne. Our hosts Marian and Briane looked after us and made sure we settled in. House has plenty of space, good cooking facility great out door eating area overlooking the valley. Supermarket a short distance away and only a short drive to the villages of Valbonne and Biot. We will be back.
Upon arrival at the majestic Villa Gezanne we were immediately greeted by the friendly and welcoming hosts Briane and Marian - it took no time at all to feel at home and every detail was well thought of prior to our arrival. The level of comfort and amenities was perfect with everything provided for a 10 day sojourn. There is ample space inside and outside in the lovely gardens for 4 couples to enjoy in absolute peace and splendour - the pool and gardens are definitely the pièce de résistance of this remarkable villa.
We ventured to nearby charming villages and on to Cannes and Monte Carlo but always longed to get back to our oasis Villa Gezanne at the end of the day.
We would highly recommend Villa Gezanne to family and friends and are already planning our return in a couple of years
Our family had a dream come true week in Briane and Marian's home.
The house was in perfect condition, comfortable beds, luxurious bathrooms, exquisite gardens, magnificent views, and a heated pool.
We had picnics and play time in the gardens with our children and cooked fresh food in the lovely kitchen.
Briane and Marian both helpful and respectful of our family time. We only saw them when we had question.
They gave us great suggestions and have a lovely family. They are also pleasant and easy to work with.
Biot has both charm and history and is located very central to many desirable towns.
It is also close to the water!
It was the perfect week.
We plan to return as soon as possible!
We have just returned from 2 weeks in Gezanne. A wonderful villa in a wonderful location. We enjoyed the warm pool and beautiful garden and were very well looked after by Marian and Briane. As we enjoy cooking, we liked the large well equipped kitchen and nearby terrace for eating. A most enjoyable and relaxing holiday.
We enjoyed a week late August with our two young children. The size of the villa is impressive and the outdoor space offers great views and a gorgeous garden. It is very safe for children as the pool area is flat and there is a pool fence and alarm if you wishes. We loved the nearby village of Biot which offers a few bars and restaurants and enjoyed many mornings sitting in the square after a trip to the local bakery for fresh warm croissants. Antibes is a great day out too. You need a car to get around but the supermarket and villages are just a short drive away. The villa is a great size for a large family and has a great shady terrace upstairs. The owners live just next door and are very welcoming but not at all intrusive. Booking was easy and the directions to the villa and any other questions and needs we had were answered quickly.
This is the most perfect villa for a large family holiday. We were a family group of five adults and six young children on our bi-annual get together.
On arrival we were so impressed with the spaciousness and also the high standard of furnishings and cleanliness. The kitchen is very well equipped and the outside terrace is a great venue for all meals. The electric awning was a boon in the very hot weather. All the rooms are very spacious and allow for everyone to have their own space if needed and there are more than enough bathrooms and showers for a large group like ours. Most impressive is the large swimming pool which we practically lived in. The children were very reluctant to leave. It is big enough to allow lots of games and the surrounding terrace has lots of loungers for those less active. The garden is very large and most attractively laid out with plenty of lawn space and quiet areas.
The house has a very special warm homely feeling and although beautifully furnished it does not feel so precious that you feel uncomfortable. It is also a book lover's paradise with a large selection on every topic. Oh to have had more time......
A large supermarket, a patisserie and a pharmacy were a very short drive away and the pretty village of Biot just a little further. We ventured to the coast but being the height of summer found it very hot and crowded. We also went to a children's adventure park nearby (which the kids loved), and being sports enthusiasts, also went rock climbing and swimming in the local river.
Marion is an excellent host. Nothing is too much trouble for her and she was so helpful but not intrusive at all. Her accommodation whilst close by is completely private and does not intrude in any way on the privacy of the villa. I would really recommend Villa Gezanne to anyone looking for a large house in this area. We do hope to return sometime - but perhaps when it is a little cooler (and as the pool is heated, anytime of year would work).
The Park families
With its excellent location this villa is an ideal summer getaway. Half way between the medieval villages of Biot and Valbonne. The villa is situated in a quiet wooded residential area, yet only 10 min from the beach, 25 min from the Croisette in Cannes or 30 min from airport and the shops in Nice. With views of the surrounding countryside and coastline.
|Rate Period
|Weekend Night
April May 2018
Apr 1 2018 - May 31 2018
7 night minimum stay
June 2018
Jun 1 2018 - Jun 30 2018
7 night minimum stay
July August 2018
Jul 1 2018 - Aug 31 2018
7 night minimum stay
September 2018
Sep 1 2018 - Sep 30 2018
7 night minimum stay
My Standard Rate
7 night minimum stay
|No additional mandatory fees
|Refundable damage deposit
A € 500 securtiy deposit is required which will be return within 10 days of you leaving the villa.
Linen and cleaning of the villa is charged separated.
Pool can be heated on request in off season an additional charge would be made for this


text:
a place for zinesters - writers and readers
November 8, 2011 from 5:30pm to 7:30pm – Central Library Small Press Love Fest Join us for an evening social with your favorite presses. Talk to local publishers to find out what's hot that they've got to get a head start on your holiday shopping. There… Organized by Laural Winter | Type: social


text:
When sticky notes and bookmarks aren't enough, we look for ways to control information. Especially tricky are the infobits that come from everywhere, including the Web, e-mail, and documents we create. One good option for this organizing chore is Evernote. Even better, they have an easy process to import all data you may have in Google Notebook. This isn't just competition, it's continuity. Google will close their Notebook soon, and Evernote gives you a way to keep that your data easily.
I really like online tools for this type of chore because they're available no matter which computer you use. Synchronizing a Personal Information Manager (PIM) from your work computer to your home computer is a giant pain, as is using your desktop-bound PIM when traveling with a laptop. With Evernote, as long as you're online, you're organized and can capture and store data to your heart's content.
Since I had been trying Google Notebook, their announcement of "ceasing development" sounded like the beginning of the end. Evernote folks say they like Notebook and many used it before they created Evernote. So they made a fairly simple import utility to gather up your Notebook data before it goes to the GooglePlex in the sky.
Yahoo's Briefcase just announced a similar plan to close. I can't find information about how many users Briefcase had versus Google Notebook, but Briefcase has been around years longer and should have many users. If you're one of them, take a look at Evernote and their "freemium" plan. One advantage of paying for Evernote is the ability to synchronize files between computers and your Evernote folders.
Evernote handles more media types than Notebook and Briefcase, so it's a worthwhile upgrade no matter what. Add in the easy import tools, and your random but required infobits may have a new home.


text:
actually,js for me,the most diffcult is the compatibility.
so many browser:(
1.load picture and get the bytearray.
2.according to binary then find TIFF tagname(tiff specification)
this is my way.but i got problem at 2.
read exif from flash.anyone done this before?read some specifications about exif,but failed still work on it.any idea?
VF said
^ The unfortunate things already happened in April 2010 and November 2011. Full time Flash jobs (focused on web browser) are in risk if not already expired. Better be prepared with different skill sets.
damn it.
all my skill is as3,flex,air.what am i doing?now i can understand why fw be so angry about adobe.
don't forget removeEventListener.if not,xml will load by twice when swf load another swf.
oh,boring monday comes again.
everytime i read something like this,i feel some unfortunately will come soon.maybe last my job:(
somebody help..
have you ever meet this,your flash works fine in every browser but ie9. i did a online flash photo editor.when you upload a image to backend.it's all fine.but in ie9.i got a null from backend.i just think of compatible. as-js problem? any idea?
i am looking for slide show.animation should be smooth.automatic play,resizehandler,fullscreen mode.no need about picture infomation.
i appreciate if somebody give me url.
oh,now ,i got ken-burn ,slip,grid mode slideshow already.need some special effects.my boss like iphoto effects
Arthropod.also a very useful tool for debugging


text:
A chef from Aldershot, who learnt his trade at the Ritz, will demonstrate his expertise in fine dining to celebrity chefs in the regional finals of a national competition.
Scott Dineen, 25, is one delicious performance away from a place in the final of the Roux Scholarship and a prize of £5,000 plus training at a three-star Michelin restaurant anywhere in the world.
The Roux Scholarship was created by brothers and Michelin star chefs Albert and Michel, to enable the next generation of chefs to train in the greatest restaurants in the world.
Most of the final 18 chefs left in the competition compete on Thursday (March 20) at the University of West London, where they will be judged by Michel along with television chefs Angela Hartnett, James Martin and Gary Rhodes.
The remaining regional finalists will cook at University College Birmingham, where Michel's son Alain Roux and two previous scholars will watch proceedings.
Scott, who has lived in Frederick Street for four years, is a senior sous chef at BaxterStorey, a fine dining catering company based in London.
Scott lives with his partner Katie Yates and three-year-old son Jack and has been cooking for nine years.
He trained at the Ritz, in London, and is currently working under head chef Hayden Groves, who won last year's National Chef of the Year competition. Scott himself made the semi-finals of that competition, but is hoping to go one better in the Roux Scholarship.
His recipe for a saddle of venison is one of those selected from hundreds of entrants and he said he was excited about being one step away from his "dream".
"It's been a hard month practising so inevitably the goal is to reach the finals," he said. "I'd love to own my own pub with a Michelin star.
"Fine dining is what my trade is but I'm up against some top Michelin chefs."
The semi-finalists will be given two and a half hours to cook their recipes, as well as a dessert from a mystery box of ingredients given to them on the day, before the judges mark them on culinary ability and presentation.
Scott said he would not let some of the world's best-known chefs put him off his cooking during the regional finals.
He said: "I met some of them last year during National Chef of the Year. You're quite star-struck, but at the same time focused on your task."
The finals will be held at Westminster Kingsway College in London on April 14.


text: Metallic Mary Jane City Flat Style: 570087528 Out of Stock Love it? Everyone else did too. This item sold out! Use our search tool, chat online or call 1.877.948.2525 to find something equally desirable. With exotic texture and a platinum shine, pointed toe Mary Jane graduates from a school girl look with honors. Faux suede trim. Man-made leather. Imported. Our obsessively great fit feels as beautiful as it looks. Breathable, no-slide lining. Super-comfortable memory foam footbed. Leather sole scored for traction. Read More… Share on Facebook Tweet Pin


text:
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|14th December 2012, 06:53 PM
diyAudio Member
Join Date: Dec 2012
Will I be able to have some one just resolder the MOSFETs in the correct manner? Or will it need a complete replacement of the MOSFETs. I think the wisest course of action for me is to request a partial refund from the seller to cover repairs for the amp.
|14th December 2012, 08:38 PM
diyAudio Member
The pinouts are different on those regulators. The positive regulator, MC7812, may have survived since input and output were swapped. Maybe not so much for the negative regulator, MC7912, because that would swap ground the output. If it were me I would replace both with new chips. LM7812 and LM7912 would work just fine.
The regulators will come right out if you take a piece of copper desolder braid a half inch long or so and place it across all 3 pins on the back of the PCB, then heat the braid with your soldering iron so all 3 pins get hot at once. Support the PCB so the part with the regs has nothing underneath so the regs can fall out. The holes in the PCB are big enough that gravity will cause them to drop right out onto the ground once the solder on all 3 pins melts. Actually kind of fun, I've done it a few times, lol.
Check that the regulators were in the right spots, too, once you get them out. The negative MC7912 is the one closest to the edge of the PCB.
Another issue is the output chips in your photo, the two in the middle, are the low power TLE2062CP option on RocketScientist's BOM rather than the standard higher current NJM4556s. The TLEs are fine, but can't drive nearly as much current to the headphones as the NJMs can. Plus have slightly more distortion, although it probably isn't audible. The details are in RocketScientist's blog here http://nwavguy.blogspot.com/2011/08/...lowpoweroption
Last edited by agdr; 14th December 2012 at 09:03 PM.
|14th December 2012, 11:32 PM
diyAudio Member
Laike - I just realized your imgr link had 4 more photos of the PCB in the gallery. In the last photo the gain op amp behind the volume control, the JRC2068, doesn't look to be seated flat on the socket. A pin may be bent underneath. Try carefully to push it down. If it won't go pull in out of the socket to make sure no pins are bent.
I didn't see your other post before. That 12Vac 200mA transformer is only a good idea if you are sure your AC line voltage is (higher end of the range) 120vac - 130vac (here in the states anyway) and your headphones don't pull more than about 5 - 10mA or so if you are also charging batteries at the same time. I've posted a long rant about it in the head-fi O2 headphone amp thread if you want the details. If you are going to place a Mouser order anyway for the regulators or NJM4556 chips you might as well get a WAU16-400 or WAU16-1000 transformer (the BOM alternates) while you are at it and toss the 12vac/200mA in the wastebasket (just my opinion here).
Good luck!
Last edited by agdr; 14th December 2012 at 11:38 PM.
|15th December 2012, 12:06 AM
diyAudio Member
Join Date: Dec 2012
agdr, thanks for your posts. I think its pretty obvious at this point that this amp isn't worth the trouble of grabbing a friend to do the repairs for me. If I could solder, I wouldn't mind requesting the partial refund and doing the repairs myself, but due to hand tremors, soldering two wires together is already a pretty dangerous task for me, let alone the PCB board. I'm going to request a full refund or for the guy to cover the full costs of repairs from a specialist. Thanks for your time and effort in all of this.
|15th December 2012, 05:16 AM
diyAudio Member
Join Date: Oct 2009
Location: Brisbane, Australia
lol, that'll teach me to not look at the schematic, many will know my O2 PCBs are still virgin. of just about all the parts the fets seem to be the most vulnerable from builder responses
Last edited by qusp; 15th December 2012 at 05:19 AM.
|15th December 2012, 07:14 AM
diyAudio Member
Join Date: Oct 2011
If you had a builder, then mistakes were made. A specialist however is not needed. An amateur like myself was able to populate the pcb so it doesn't take a rocket scientist. Just attention to detail.
|15th December 2012, 05:28 PM
diyAudio Member
Indeed, no specialist needed. If we lived closer to eachother I could easily fix it, and I'm a beginner (built a couple of O2 amps though). Maybe you have any friends that can solder?
My audio and DIY blog: http://phimusic.blogspot.se/
|22nd December 2012, 03:10 PM
diyAudio Member
Join Date: Nov 2012
Location: Sichuan
Very appreciate Mr.RS's effort on O2.AMP!
I build one by myself in China. the O2 sound is fantastic. I am very like it. low cost but the performance is not low at all, there are many many lies in audio industry, one you need pay a lot for high performance sound. I am on same side with RS, I don't believe human ear can hear the difference between a normal power cable and a Hi-End cable that maybe cost hundreds dollars.
I'd like to encourage the peoples who still hesitant if you want build an O2 AMP by yourself, you don't need any knowledge or background on analog circuit, Mr. RS already did everything for you. the only thing you need is careful, when you put components on the PCB. and some solder practice.
But I still have some questions about the O2 AMP, I am very appreciate if someone open discuss.
In my view the current O2 design is a portable and desktop mix version. I like a portable AMP more, so I'd like to know, is there way to MOD the O2 to make it more like a portable AMP, it work with Li-ion battery and DC battery charge circuit. sounds this requirement is more like a new version AMP.
Actually I accept the current Ni-Mh battery design, but my concern is it keep to charge the battery and wont stop even battery is full if we plug the AMP to an AC adapter.
If we use Li-ion battery we can share a same charger with cell phone, even charge the battery by the computer USB.
|23rd December 2012, 09:27 AM
diyAudio Member
Join Date: Oct 2011
Hi Archro - there was a discussion about using Li-ion batteries early on in the project, but the difficulties of charging them safely ruled them out. Get the charging circuit wrong, and they can explode! It's much easier to charge NiMH batteries safely, making them more suitable for inclusion in a device that's intended to be built worldwide, by people of vastly differing experience.
If you were going to modify the circuit to include Li-ion batteries, it would be necessary to include extra electronics to monitor the charge state and charge history, along with the necessary microcontroller - much more complicated and expensive to build.
There's no way to modify the circuit to charge from USB, the voltage is too low.
Last edited by greenalien; 23rd December 2012 at 09:31 AM.
|25th December 2012, 12:24 PM
diyAudio Member
Join Date: Nov 2012
Location: Sichuan
Hi Greenalien, thank you for your replay.
I know we cannot use USB to charge current equipment.
for the Li-ion battery, I think there are billions people use this battery in the work, most of them no EE background, but I am totally understand your concern.
so is there any way to mod the charge circuit to add an auto-stop function.
I have a new question after build.
I use 0.22uf for C6 and C7 because this is what the diagram shows, but in the BOM file there are 1uf Cap. so I'd like to know whic Cap should be better.
also my C6 C7 CAP are film caps, does this matter?
thank you for your any replay.
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text:
Thanks to the nomination by a student, Biology Professor Kristian M. Hargadon '01 has been honored with a Sigma Xi Fresh Faces of the 125th Anniversary Award. Sigma Xi is a society to honor excellence in scientific investigation and to encourage companionship and cooperation among researchers in the sciences and engineering. Founded in 1886, Sigma Xi has more than 60,000 members around the world.
The Fresh Faces of the 125th Anniversary Award recognizes 125 Sigma Xi members under the age of 35 that have shown promise in their respective field of study and dedicated contributions to the society. This past summer Osric Forrest '12 nominated Dr. Hargadon for the award and Dean of the Faculty Robert Herdegen wrote a letter of support.
Since returning to Hampden-Sydney College as a professor in 2009, Dr. Hargadon has become very popular with the students. His demeanor is comfortable but professional and, as a graduate of the College, he has a great understanding of what it takes to be a science major on The Hill. While an undergraduate, he collaborated with researchers in the laboratory of Dr. Victor Engelhard at the University of Virginia, where he subsequently conducted his Ph.D. research on the induction of CD8+ cytotoxic T-cell responses to melanoma. His research during this time resulted in two publications in the peer-reviewed journals: The Journal of Immunology and Immunological Reviews. His work was also presented at a number of scientific conferences, including the International Congress of Immunology in Montreal, Canada, in 2004. After completing his Ph.D., Dr. Hargadon joined the laboratory of Dr. Thomas Braciale at the University of Virginia for a postdoctoral fellowship. His love of research and teaching led him back to Hampden-Sydney where he has established his own research program and continues collaborating with, mentoring, and guiding undergraduate students.
Dr. Hargadon says, "To have been nominated by one of my students makes this recognition all the more rewarding. A major mission of Sigma Xi is the promotion of scientific research, and I feel very fortunate that Hampden-Sydney has afforded me the opportunity to train our next generation of research scientists."


text:
|Mccollum, Thomas
|Maul, Dora
Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/22/2006
Publication Date: 2/1/2007
Citation: McCollum, T.G., Maul, D.P. 2007. 1-Methylcyclopropene inhibits degreening but stimulates respiration and ethylene biosynthesis in grapefruit. HortScience. 42(1):120-124. Interpretive Summary: Ethylene is a gaseous plant hormone that affects many aspects of plant growth and development. The role of ethylene in ripening of climacteric fruit (e.g., tomatoes, bananas, apples, avocados) has received considerable study and its role understood. In contrast in non-climacteric fruit (e.g., grapes, strawberries, citrus) little is known about the role of ethylene. Citrus fruit do produce ethylene and ethylene is used commercially to 'degreen' the fruit; however, our understanding of the role of ethylene in citrus fruit development is limited. Our objective in these experiments was to define more clearly the effects of ethylene in citrus fruit. We treated grapefruit with 1-methylcyclopropene (1-MCP) a compound that inhibits the perception of ethylene, and thereby blocks ethylene action, in order to gain insight regarding the role of ethylene. We determined the effects of 1-MCP at different doses and also the effect of 1-MCP alone or in combination with ethylene. We found that 1-MCP has very pronounced effects on grapefruit. Ethylene-induced degreening was inhibited by 1-MCP. However, 1-MCP treatment caused an increase in the production of carbon dioxide and ethylene, suggesting that perception of ethylene may affect respiration and is required to regulate production of ethylene by grapefruit. Understanding the role of ethylene in citrus fruit has both fundamental and economic importance. Ethylene is used commercially, but can lead to increased losses due to decay. Developing a better understanding of the role of ethylene in citrus fruit physiology will allow for the development of treatments to reduce postharvest losses of fresh citrus fruit.
Technical Abstract: We determined the effects of 1-MCP and ethylene on color change and CO2 and ethylene production in grapefruit. Treatment with 1-MCP at concentrations equal to or greater than 75 nL/L inhibited ethylene-induced degreening, but increasing 1-MCP concentrations greater than 150 nL/L did not cause additional inhibition of degreening. Although ethylene-induced degreening was inhibited by 1-MCP, the effect was transient. Treating grapefruit with 15 ' 75 nL/L 1-MCP resulted in a slight suppression of CO2 production whereas treatment with 150 or 300 nL/L 1-MCP resulted in rates of CO2 production significantly higher than non-treated fruit. 1-MCP treatment also caused a very pronounced increase in the rate of C2H4 production that was dose and time dependent. The effects of 1-MCP on respiration and ethylene evolution were significantly reduced if fruit were subsequently exposed to ethylene. Fruit treated with 1-MCP alone had the highest rates of CO2 production, fruit treated with 1-MCP + ethylene or ethylene alone had intermediate rates of CO2 production and control fruit had the lowest rate of ethylene evolution. Rates of C2H4 evolution were ca. 200 nL/kg h-1 from control and C2H4-treated fruit compared to ca.10,000 nl/kg h-1 from 1-MCP treated fruit; fruit treated with ethylene following 1-MCP had ethylene production rates of about 400 nL/kg h-1. Our results lend further support for a regulatory role for ethylene in degreening of citrus and suggest that endogenous levels of ethylene regulate ethylene production.


text:
Multnomah University prepares students for meaningful careers that serve Jesus whether they're called to traditional ministry or the marketplace.
Stay connected to our community and help the next generation of Christian leaders become agents of positive change, whether they're serving across the street or across an ocean.
Multnomah professors will take part in the annual missions conference, Mission Connexion, by teaching a variety of workshops. The professors and their workshop topics are listed below.
Dr. Martin Alphonse: Engaging the Gospel in the Hindu Culture
Dr. Greg Burch: Becoming a Community for Children at Risk
Dr. Karen Fancher: God in the Midst of War: Reflections from Southern Sudan
Dr. Elizabeth List: Staying Sane in Missions: How to Keep Missionary Families Healthy on the Mission Field
Dr. Roger Trautmann: Creating Transformational Cross-Cultural Partnerships


text:
GOMORRAH canceled; BURMA VJ screening added!
We unfortunately had to cancel our 2 screenings of GOMORRAH, because of a schedule overlap, the print will not be available. We're looking for a replacement for the two show times that GOMORRAH occupied. GOMMORAH will be opening in Honolulu in early May, so you will be able to see the film then. We have added a new film called BURMA VJ, details about it are listed below.
BURMA VJ (DENMARK – 85min.)
TUE. April 7th | 9:15pm
Armed with pocket-sized video cameras, a tenacious band of Burmese reporters face down death to expose the repressive regime controlling their country. In 2007, after decades of self-imposed silence, Burma became headline news across the globe when peaceful Buddhist monks led a massive rebellion. More than 100,000 people took to the streets protesting a cruel dictatorship that has held the country hostage for more than 40 years. Foreign news crews were banned, the Internet was shut down, and Burma was closed to the outside world. So how did we witness these events? Enter the Democratic Voice of Burma (DVB), aka the Burma VJs. Compiled from the shaky handheld footage of the DVB, acclaimed filmmaker Anders Ostergaard's BURMA VJ pulls us into the heat of the moment as the VJs themselves become the target of the Burmese government.


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Snowboarding Forum - Snowboard Enthusiast Forums (http://www.snowboardingforum.com/forum.php)
- General Travel Forum (http://www.snowboardingforum.com/general-travel-forum/)
- - Snowshoe Resort or wait and go to Appalachian mtn? (http://www.snowboardingforum.com/general-travel-forum/52161-snowshoe-resort-wait-go-appalachian-mtn.html)
Snowshoe Resort or wait and go to Appalachian mtn?
I'm supposed to go to Appalachian Mountain this weekend to snowboard which is only a 3 hour drive for me. They might not have snow though. Would it be worth it to drive 6 hours to Snowshoe in WV or wait for snow at Appalachian? If I miss this weekend, I will have to wait until the last weekend in Dec. to go anywhere again.
Go to Snowshoe. Are you planning on staying the night? 6 hours isn't bad if you stay for more than a day.
Well, I just got a call from Appalachian and they are going to be closed this weekend. I was going to go up on Friday, ride, stay the night, ride sat and come home that night.
If I go to snowshoe, where is a good place to stay that is cheap and clean?
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I stay at Expedition Station when I go there in a junior studio it has a bed on the floor and a fold out sofa a 2 burner stove top and microwave and sink. You can also stay at Siver Creek lodge I have never stayed there or at the SnowShoe Inn if you stay at the in you have to drive up to the resort there isn't a shuttle bus that goes down there.
I'll be driving and will have my car so I can stay "off the mountain" and just drive back to the ski slopes. Any good best western or holiday inn type places that are good? I will be traveling my myself, so I don't need a big room or anything.
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The only off resort lodging that I know of is the Marlington Inn.SnowShoe is in BFE.
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I am pretty sure they have smaller rooms.I would call them up and see what they have.:thumbsup:
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Tag: cosmos
Marcus DeSieno on Merging the Old and New, Manifesting the Unseen, and Exploring the Vastness of the Universe with Photographywritten by Julien Matabuena on 2015-03-04 #people
Marcus DeSieno is a Tampa-based photographer who specializes in merging early and modern photographic processes for his body of work. In this exclusive follow-up feature, DeSieno opens up about his process and gives a detailed walk through on his odd yet undeniably fascinating series, "Cosmos," which was previously featured here on the Lomography Magazine, and "Parasites."5 18 Share Tweet
Marcus DeSieno's 'Cosmos'written by Julien Matabuena on 2015-02-18 #people
In this aptly-titled series, one photographer attempts to comprehend the vastness of the universe through a rather unusual process.5 15 Share Tweet
Fukuoka Airport, Japanwritten by unknownsoldier on 2010-12-03 #places
Fukuoka is located in western Japan; due to its proximity to Korea as well as China, Fukuoka is positioned to become the next tourism and trade hub in Asia. This airport is also considered to be the "gateway to Asia", but if you're just spending a lazy afternoon, no need to go inside, just look for a comfy spot near Fukuoka Airport and instantly get an amazing view of the planes that are departing - and landing in the busy airport.5 12 Share Tweet


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Rational Nation USA
Liberty -vs- Tyranny
Yep, certainly everyone should be able to agree on the need to offset the cost of Sandy's devastation to the federal budget. As well, everyone should be able to see the logic of the states effected shouldering a share of the burden of cost.
House conservatives are wasting no time in demanding that emergency spending for Hurricane Sandy be offset by spending cuts to the rest of the budget.
The White House on Friday requested Congress to pass a $60.4 billion bill to pay for damage resulting from Sandy.
Speaker John Boehner (R-Ohio) and other GOP leaders have so far been mum on whether they will demand spending cuts to match.
The right wing members of the House conference are not waiting to make their feelings known.
Rep. Cynthia Lummis (R-Wyo.), who sits on the Appropriations Committee, said she will need to see offsets on Wednesday as did Rep. Raul Labrador (R-Idaho).
"We have these emergencies every year and we should prepare for that in our budget," Labrador said.
"No pun intended, we should have a rainy day fund," Rep. Jeff Landry (R-La.) said.
The August 2011 debt deal allows the Federal Emergency Management Agency's disaster relief fund to have up to $11 billion this year in extra funds for a disaster, but this money is not enough for the historic damage cause by Sandy according to the governors of New York, New Jersey and Connecticut.
Rep. Steve King (R-Iowa) said he will be looking for offsets for at least some of the spending and indicated he is not comfortable with the White House request that state and local governments contribute less than 25 percent of the cost of repairs. {Read More}
Something came to mind immediately. The DoD.
Via: Memeorandum


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Phrasal Verbsphrasal verbjump to other results
screen offphrasal verb
to separate part of a room, etc. from the rest of it by putting a screen around it Beds can be screened off to give patients more privacy. The choir is screened off from the rest of the church.jump to other results


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Giant Skull Made Out Of Kitchen Utensils
I'm a fan of making the most of a situation when possible. At least in terms of decor. So if I find myself stranded on a desert island, I want to go gung-ho with the theme. This artist's sculptures are great inspiration- I just need to collect all of the cans and bottles that wash up on shore and voila! Beach art that exudes a cannibalistic aura.... It says, "Hi. I will eat you."
Luxe Alexander McQueen skull clutch in black crystal embellished satin with a dusty pink Nappa leather lining. The skull has purple river pearl eyes and smoke Swarovski teeth and studs.Added 8 Years Ago from Alexandermcqueen


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CytoSport Monster Creatine - Watermelon
Product Overview
CytoSport Monster Creatine - Watermelon
CytoSport Monster Creatine - Watermelon
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CytoSport Monster Creatine - Watermelon
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Part Number(s): 827 583981 01
Mfg Part Number(s): 400361
Scrip Part Number(s): 827 583981 01
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Cast:Angelina Jolie, Liev Schreiber and Chiwetel Ejiofor and others
Director: Phillip Noyce
Writer: Kurt Wimmer
Genres: Action/Adventure, Drama and Thriller
Running Time: 1 hr. 39 min.
Release Date: July 23rd, 2010
Watch Salt (2010) Online English DVD Movie :
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Before becoming a CIA officer, Evelyn Salt swore an oath to duty, honor, and country. She will prove loyal to these when a defector accuses her of being a Russian sleeper spy. Salt goes on the run, using all her skills and years of experience as a covert operative to elude capture, protect her husband, and stay one step ahead of her colleagues at the CIA.
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Two young girls gets punished for shoplifting
They've been a problem in the small Maine seaside town of Kittyquit for a
few years now. I'm talking about shoplifters during spring break. More
persistent than blackfly, more intrusive than a wrinkly-laden Winnebago
with Arizona plates on a narrow road. What annoys the local shopkeepers
most of all is, they don't do it because they need the money. Although that
would be bad enough. They just do it to amuse themselves. Clothes are often
worn once and consigned to hotel dumpsters, or tossed unworn because they
must have been the wrong size.
Last year, the local Chamber of Commerce, with the grudging 'look
the other way' approval of police chief Tom O'Reilly, decided they'd have
to try something different. They'll make extra efforts to catch and make an
example of some offenders, as a deterrent. Chief O'Reilly commissions some
local craftsmen to build a pillory, shows it for a few days on the neatly
clipped lawn outside the Kittyquit firehouse. It's almost art. There it
stands, menacingly, with a public noticeboard detailing the kind of
offenses that will be rewarded with it. The locals nod approvingly. Yes,
this idea of shaming offenders has a long history in these parts, stopped
only too recently by do-gooders and social scientists. It's time for a
comeback. Next spring, the pillory will be brought out daily as a reminder.
And used, for sure.
Jenny and Laura are spoiled rich kids from a very famous Beantown
area college, which we'll call Bumherst. They're 21, and should know
better. Jenny's daddy is some scumbag NYC divorce lawyer, Laura's is a real
estate developer in California. Jenny's mummy was composted long ago,
Laura's young (step)mummy is a high-priced whore — oh, slap yourself! — I
mean 'socialite and volunteer.'
The two girls are morally bankrupt. They're careful about what they
do at home, careful in the city. But they often lift clothes as a dare,
rather than pay for them, when they're travelling. Mostly, it's an impulse.
It's not because they are splendidly dressed, or elegant, though if the
truth be told their ratty clothes are quite expensive.
They epitomize the slacker mode in baggy jeans, sneakers, baseball
caps on backwards, four or five layers: plaid flannel shirts, tee-shirts
and sweaters. But, careful makeup, manicured painted nails. "We is
slackettes a la mode," Jenny has said. Today, they're in a bubbly, giggly
mood enhanced by a few tokes in the car on the way from the motel out on
Route 1. The style of the clothes they've been seeing so far this year is
dull, compared to the big city, so they'll probably be boosting jewellery
today, they've already decided. "Baubles, for my princess-sss, yethsssss,
my preciousss, gollums," Laura is chuckling. "If you kiss the magic ring,
the dragon's nasty poopy ring," Jenny agrees.
They're smoking, chewing gum, singing along with the Recombinant
Turds on the car radio. It's a fine day to be young, up to the wazoo in
easy money, and out about your business. Jenny is small, dark, intense. She
has a hispanic vivacity — even carries a fake ID that says Juanita Garcia
— but she's part Italian, part Jewish. Laura is a tall stately blonde,
icy, cynical, her grandparents all Minnesotans. She's not bothered when
people call her a Brunnhilde, a Viking. Yes, they do sleep together, but
it's not love, or anything profound or serious, just an itch-scratching
thing: They have varied interests in sexual matters. There are boys, other
girls. In the distant past now, there was even Laura's aptly named and
rather mixed-up Doberman, Licky.
But Jenny and Laura aren't as clever as they think. Oh, they're not
up against a lot of smart systems doing the shoplifting routine in these
little towns, like they do when they go 'grab and run' at the malls, or the
outlet stores. No spy cameras, dye-loaded tags, little magnetic strips and
embedded printed circuit thingies, no scanners, all that high-tech
bafflement. No, just a few busy shop clerks, working moms, who have dozens
of things to worry about at any given time. But being observant comes
second nature to some. And to the poorer, non-college-educated, there's a
deep resentment against rich young trollops in late-model cars. Oh, it's
only a '95 Acura, but that's a ritzy set of wheels to a woman who owns an
'82 Lynx with 150,000 miles on the clock.
And it's just such an automotive cognoscenti who nabs them.
They're in one of the smaller local boutiques, when Katie, always quite
observant, catches them slipping a broach into a brown bag with a coffee
cup in it. A quick grab and she has Laura's wrist in a vice-like grip.
Jenny makes a run for the door, but is tripped and ends up in a heap on the
floor with two sizeable female assistants sitting on her. Lots more 'stuff'
is found with a quick search. There are huffy protests about 'due process,'
and then even desperate pleas to be allowed to pay. With a gold credit card
of course, though it's even just possible Katie might have let them go if
wads of folding green stuff had appeared. But they're locked in a store
room, and the owners called in. No explanation will satisfy Sara, when she
arrives. They've missed their moment. She's very angry. The fortyish
co-owner is tall, thin, well-exercised, beautifully dressed, with sharp
features, elegantly highlighted greying hair. She's of patrician background
too, but is here by choice, not to steal.
Soon, police chief Tom O'Reilly has arrived, too. He's annoyed, he
was planning on spending the morning eating donuts, and the afternoon
stuffing his mistress's donut. Now, he's had to drive 17 miles here, all
for a couple of damned shoplifters.
The two felons are escorted to the Kittyquit town center. The
center is little more than a huddle of boutiques in converted houses just
off a main road junction, just a way back from the public beach, with a few
restaurants, a tiny cinema, a chocolate shop and lots of twee signage.
O'Reilly listens to Sara's rantings, her insistence that this time he must
do something. With a shrug, he says: "Alright. Let's try it. This pair may
not have seen the new rules, but ignorance of the law was never an excuse,
anywhere. The rules were posted, quite legally, for oh, at least three
weeks. Right? And published in the local paper. So, we have the right to
act, under the local by-laws. It'll save us a lot of trouble booking them,
and then not having them show up when it comes time for the magistrate's
hearing in a month or so. We don't get enough convictions anyway. Okay,
Sara. You win. We'll proceed."
The two girls want to protest at length, being the kind of
motormouthed know-alls they are. But they don't, fearing the brooding
violence that they see gathering around them. And knowing that there is
some illegal herbal matter hidden, not so well, in their car. Just to get
this over and be gone from Kittyquit would be a good move.
They're taken on to the green by the firehouse, where they're shown
the pillory. They're horrified. The set-up is a set of old-fashioned
stocks, with head and wrist restraints, and heavy ankle shackles. They'll
be on show there, standing for a couple of hours, O'Reilly tells them
gruffly, to teach them some honest habits.
The stocks are of side-by-side construction, made of heavy,
weathered 2×6 planking and house timbers, freestanding. While the two girls
been locked in the storeroom, their purses have been searched, and all the
necessary details taken. Those are going to be posted on a notice board
here, they're assured, and their mugshot photos are taken for a 'thief'
file to be handed to shops in billages all along the coast, and to be
printed in a local freesheet. It's assumed this will be enough to stop them
ever coming back, O'Reilly lectures. And then, he's gone. For donut, v.2.
Visitors and locals all watch their attachment to the pillory with
approval. The two are shielded behind a large portable wire mesh cage, like
a hockey goal, so they can't be 'interfered with' or pelted with rubbish.
That's purely for safety and liability reasons, something one of the town's
thrifty accountants had insisted on. But they can be jeered at, and mocked
with impunity. If it was summer, there'd be a huge throng: thousands of
visitors, and coachloads of tourists on coach trips from Montreal and
Quebec, Boston and other nearby resorts. Plus the gawkers in the endless
slow-moving permanent traffic jam of the coastal, highly unscenic route.
Instead, there are only a few dozen viewers at a time. But that's enough to
chill the two lightfingered young ladies.
At the end of the day they're to be freed as the shops close.
That's O'Reilly's intention, anyway, and that of the other rulemakers. But
they're not around to supervise when the time comes round. He's far away,
and busy. The crowds have thinned out, and the two transgressors are rather
stunned, tearful even. They are sure they won't be doing any stealing
again, and never coming back here if they can avoid it.
That's the intention of others, too. As the more conservative,
restrained townpeople and Chamber of Commerce types leave to go home for
supper, with a last scowl or shrug at the plight of the duo, some younger
ones, counter staff, waitresses, school bus drivers, the local coffee shop
crowd, are still hanging around.
Boutique owner Sara is there too. She has lost thousands of dollars
in business over the past two seasons, and is determined she will not let
them escape so lightly, or so easily. She's long planned how, and she puts
her plan into effect.
The stocks are heavy. A half-dozen guys would strain to lift them.
So they're hydraulically lifted on to a big flatbed truck, to be taken back
for overnight storage at the local Kittyquit school gym. That's what was
always planned, and the truck backs up, beeping happily, right on schedule.
But here's the difference tonight: Jenny and Laura are left
standing in them. When they start to protest, to shout out for help,
they're firmly gagged. A huge painters' dropcloth is placed over them and
roped down. The truck pulls away.
Several cars and pickups follow, lights on, to the local school.
It's a holiday week there too, so it would be usual if the school was quite
deserted. Not tonight. When the truck pulls round the corner, the car park
is crowded. Sara and others have been on the phone, and word spreads
The truck backs up to the door and the stocks are maneuvered onto
the rear tailgate lift, and lowered on to a trolley. When the dropcloth is
pulled off, there's applause from the group of followers who've parked and
joined the festivities. The trolley is wheeled away, with the two hapless
young women facing backwards, craning and struggling. Up a steep ramp, with
lots of huffing and puffing. Noisily rattling along an echoing, tiled
corridor. Through two sets of swing doors, with big clear plastic draft
protectors slapping away. Now, they're inside, somewhere warm. The gym.
It's quite small, with a few steeply angled rows of orange plastic seats
making an amphitheater.
The gym is packed with guys, though there are several huddled
groups of women too. It's bright, but as the trolley and its cargo appears
on court the lights go up properly, and the arena is lit as bright as
summer, for indoor basketball. There are about eighty guys, young, old,
longhaired, bearded, crewcut, balding, in various states of excitement, or
Sara is there already, and briskly supervises parking the trolley
on the half-way line. She has a microphone in her hand. She notes with a
cynical smile: "Welcome, friends. Oh, and a warm welcome to our 'guests,'
Jenny and Laura. Nice of them to join us, eh?"
Some derisive cheers, and a feeble patter of handclaps.
"Well, now. These two thieves are really well dressed, aren't they?
Did you notice? But, uh, has it occurred to you, too? That, perhaps the
clothes aren't their own? Yes?"
There's mean laughter at this. Oh, everyone gets it immediately.
"Maybe we should check some labels, hey? It's possible that some of
this cityslicker elegance may be, well, at our expense. Look at them
blushing! Not laughing at the hicks in the country now, are they?"
And then they are forcibly stripped, piece by piece. Yes, Sara and
her staff have to unfasten the girls' ankles to take their jeans, sneakers,
socks off. But with their arms still locked in, where can they go?
Struggling makes no sense. Then, ankles locked in the chains again, their
arms are freed so that their sweaters, flannel shirts, teeshirts can be
slipped off. Both the girls are growing more frantic as the clothes are
inventoried, inspected. No definite thefts have been detected yet, but the
shopkeeper vigilantes live in hope. Tags are carefully read.
"This Woolrich seems familiar, hmmm?"
"Didn't we stock this brand last year?"
Soon the lightfingered duo are both nearly naked, dressed now just
in their bras and panties, and the cheers are ringing out.
Sara is in a mocking mood. She knows she has them at her mercy now.
She forcefully stretches and twangs both girls' bra straps, making them
flinch. She grabs and wrenches the back of Laura's panties upward, pulling
the gusset snugly into the crack of her ample ass. It amuses her that this
grungily outfitted pair would wear such conventional undies, but there they
are: peach silk, and black lace, dainty filigrees and embroidery, for
Jenny, bikini cut panties and an underwired bra for Laura. The usual female
priorities, in other words.
This unveiling is the best sport here in weeks, months, that's
plain from the laughter, the nudging, the excited expressions. Older guys
who haven't had a decent erection since Christmas are showing big bulges in
their jeans.
Now the stocks are adjusted so that the two students' arms are
stretched out more. Some new wooden beams are brought, and bolted into
place at ankle level, and they are forced to place their feet in the
openings in them. It's all configured so that they must open their legs
much wider. They're spreadeagled, completely helpless, their heads still
locked in place. The crowd is very boisterous, and there are merry shouts
for 'more' from the guys. The women are just as positive, insisting that
Sara's team keeps going.
That's okay by Sara. She produces a big pair of carpet shears,
brandishes them in the air, leers in comic opera fashion. She points to a
wet patch on Jenny's silk panties and says loudly: "They're not very
genteel, our guests. I wonder what this one is thinking? But, ha ha,
perhaps we don't want to know, eh? I think we should take the rest off just
to be sure, don't you?"
Their bras and panties will be easily removed, no effort at all, by
just snipping them off. And so, they are. The unveiling of Jenny's small
breasts bring groans of derision, disappointment from the men, though
connoisseurs don't miss the message of her fat, erect, dark nipples.
Laura's big firm jugs bring ironic cheers, derisive 'mooing' noises. Her
areolae are pink, huge, maternal even. Sara weighs Laura's breasts in her
hands, squeezes, prods. "They're real, believe me," she assures the crowd.
There's a slow handclap going, and whistles and hoots greet the
destruction of Jenny's panties. Her huge untrimmed black bush impresses the
guys, and makes some women laugh aloud. Who'd have guessed? Sara tweaks at
it gently, asking: "Is this real, or did you steal it from a wig shop?"
then knots her fingers into a tight fist and gives it a good hard pull. She
twists and shakes hard enough to move Jenny's hips back and forth, just to
be sure. Tears fill Jenny's eyes.
Twice would be too much to hope for, so both camps choke with
laughter when Laura's freshly shaved, waxed genitals are revealed. Women
here don't shave, it's plain. They're fascinated at the sight, like some
pornographical porcelain sculpture. Sara rudely parts the young woman's
pouting labia, as if looking for lost car keys, to show her pink slit, then
wipes her messy fingers clean on her belly. Now the two girls are naked,
and crimson with shame, there's another five minutes of clapping, cheering,
slobbering and mockery. Finally, Sara raises her hands for quiet, and calms
the crowd down enough to announce their fate.
She introduces three other women, Jane, Pauline, Wendy, all shop
owners, all unforgiving victims of last year's wave of robbery. Were these
two responsible? They don't care. They'll do just fine, as an example to
others. They'll be assisted by the observant Katie, who didn't like their
attitudes, and another couple of shop helpers.
The girls are told: "Now, Jenny? Laura? Are you both ready? Paying
attention, are we? Now, we've got you where we want you. And it's time for
your proper punishment. Yes. I don't think being made to stand in the
pillory counts for much, frankly. Not compared to the mischief you've been
up to.
"So, let's get serious, shall we? You're at the school here for a
reason. To learn a lesson. We are going to paddle you, and birch you, like
the lowdown, petty thieves you are. Not just a little bit, either. Oh no,
you snotty little bitches. We have the time to do this right, and we will.
You're going to be beaten until you are good and sore. In fact, we're going
to beat you until we see you cry, like the spoilt children you are."
Their eyes are wide with horror. The crowd loves this.
"Then," she says, with a nasty smile, "the men here will be
permitted to administer a little adult punishment, too. Something
demeaning, insulting, something you'll remember with distaste for a long
while to come."
There's a long, hungry silence. Just one woman giggling. Sara says:
"Since you're quite old enough, we won't impose any prudish limits, but I'm
merely suggesting they confine themselves to, well, something appropriate.
I think a healthy dose of penetrative and humiliating punishment would be
good, myself. I mean, I think it'd be best. Rather than whipping the two of
you any more."
"But, you know?" she shrugs, "It's up to them. I don't make the
rules. If they think you need a bit more ass-warming . . .Hey, why would I
spoil things? I'm just arranging things, making them possible. I'm not here
to protect you. Quite the opposite, really . . ."
How much corporal punishment will they get? It's plain that's what
the two are anxiously wondering now. They're not asking aloud, because
they're still snugly gagged for this part of their ordeal, and will remain
It's apparent that small, dainty Jenny is going to get it first,
because she looks the most scared. It's merely warm in the gym, and you'd
think a naked girl would be shivering, from cold or fright, under these
circumstances. But Jenny is sweating heavily, and perspiration is trickling
from her armpits. To start with her will make Laura suffer, and give her
friend something to anticipate and worry about too.
Sara takes up a black felt-tip marker. She looks Jenny over
carefully, enjoying her fear. She tousles her boyish floppy black hair, and
asks mockingly: "Scared, dear? Well, good . . .I'm glad to see it." Sara's
own preferences have long been discussed in town, since her recent divorce.
Her first husband had been a famous writer with a drinking problem, who'd
driven his Cadillac into a saltwater marsh one day. The second, a local
cocksman, had shot himself. An accident? Maybe. The third, a reclusive
Southerner, he'd drank, moped, and in the end just left quietly. Tall,
elegant Sara indicates what her deepest inclinations might be when she
gently pokes her tongue in Jenny's ear, and cupping her small breasts in
turn, twiddles the boldly erect, chocolate brown nipples. Sara runs her
fingers lightly over the girl's shoulders, her back, down her spine, and
tenderly strokes her small rounded backside. She whispers, so only Jenny
will hear: "So soft. You're a fuckable little thing, aren't you?" Jenny
draws a deep breath and her eyes close in fear as the woman breathes:
"Horny, are you? You think I can't smell you? Your filthy snatch, hmm?"
Sara is tempted, but has to suppress the urge for further
exploration. This isn't the time or place. Standing back, she begins
speaking softly, perfectly miked by one of her assistants. "So, here's the
plan, Jenny. We introduced you to the idea of Shame, first. Not many women
get to exhibit their bare bodies like this, only the ones who are complete
sluts, or who deserve to be degraded and laughed at. Like you. Then, Fear.
Because you are scared now, aren't you, Jenny? You know we have you at our
mercy, and that we can do whatever we want." A pause, a sigh of
contentment. "And, so, quite naturally . . . then it's time for Pain, isn't
it? Lots of it. You need to be hurt. Hurt badly, so you don't turn into a
sociopathic little thief, who thinks everyone else is there just for her to
exploit . . . like some greedy rich bitch!" And as she spits the last few
words out, she writes, in huge shining black numerals, "150" on her back,
"125" on each cheek of her ass. The marker squeaks as it slides over
Jenny's skin.
There's a murmur of excitement. Laura twists to look, gasps, her
eyes rolling up in shock when she sees what's written. Sara tells Jenny the
numbers, then with a big smile breathes: "Oh, and by the way, that's the
minimum." Then she steps in front of her, the pen still in hand.
No! No! Jenny's panic-stricken eyes are saying. Yes, Sara is
nodding. A mean little smile, a flick of the tongue to remove the fresh
saliva at the corner of her mouth. She bends forward.
Slowly, a neat arrow is drawn, pointing right at Jenny's bushy
pubes, and the number "75" added in a circle, next to her perky little
navel. A smile, then she harshly says: "This dirty thing needs a damned
good pounding, I can tell."
Then "50" on each thigh. A large "60" on each small breast. Jenny
can see this, and her eyes are wide with disbelief. She's dizzy, and it's
not the smell of solvent from the pen that's doing it. Sara confidently
tells her: "Too cruel? No, not at all. Jenny, we're going to purify you,
make you feel sorry for your sins. So, you have to realize, if it doesn't
hurt, you won't understand it." She does some mental arithmetic, and smiles
crookedly: "695, that right? Well, that's awkward. Oh, we'll round it up a
bit, I'm sure . . ."
Now, the instruments are produced, and shared out. Two huge bundles
of stiff birch twigs, freshly soaked in water. There are replacements
waiting nearby, in a big bucket. Two spanking paddles of the sort used by
the school system in the forties . . . homemade, sawn-off tennis racket
handles attached to broad stiff leather blades — 18 inches long, four
inches wide, stiffened with a wooden spine on one side.
Sara takes a paddle. She tells the others, loudly enough for all to
hear, "No playing around, please. Keep a rough score and we'll even up
later. And remember, no mercy. She may be a cute, cuddly little thing, but
she's a thief, caught in the act. We're going to whip it out of her. So,
just remember that and beat the bitch hard . . ." There are growls of
And they don't mess around: she's birched and paddled, front and
back, and is soon shaking and hysterical, her pale skin heavily marked with
angry red patches and stripes. Of course, spanking her ass is very popular,
but so is birching her pussy and letting her breasts have it, with birches
or the paddles. With four women busy, it takes them about 40 minutes to
beat her to their satisfaction, perhaps going a little over the quota in
their enthusiasm. No matter how much Jenny writhes or twists, there's no
escape, not even time to catch a breath when four woman are swinging at
her, almost all at once.
Then, they turn on Laura, her big blonde companion. She's Sara's
height, and tries to meet her eyes, retain some defiance. That's fine by
Sara, who smiles right back, and proceeds to slap Laura's face, back and
forth several times, saying: "The bigger they come, the harder they fall,
eh? I'll tame you, you big bitch!" Laura finally lowers her eyes meekly.
But she's earned a harsher penalty. Larger numbers are written on
her big backside and impressive bosom, adding up to about 1,000 strokes, to
the crowd's delight.
The four women promptly start to beat her vigorously. Soon though,
the couple of women wielding the heavy birches are complaining their wrists
are sore. Some robust guys are deputized, and vehemently told by Sara:
"Really let her have it! Flog her, she's too full of herself." Laura is
grunting and moaning through her gag, muffled shrieks of outrage. Special
care is taken to give Laura's large breasts a proper treatment: some extra
handslaps, some nipplepinching from Sara. A few dozen extra slaps to her
shaved mons with a paddle, again applied by Sara personally, with great
skill and venom.
Both girls are red-faced and sobbing when they're done.
"Oh, my word! What a display of temperament! But, my little
looters, that's only the beginning! Please, you really must pull yourselves
together, or you'll run out of tears," Sara tells them.
Now one of the other boutique owners appears with a pair of long
bamboo canes, to a polite round of applause. Over three feet, tapering from
an inch or more in diameter at the leather wrapped handle end, to a
fishing-pole-like quarter-inch at the tip. Sara takes one and swishes it
noisily through the air like a fencer's foil, then pronounces: "Ah, this
will do very nicely. Yes, I think they'll get the message from this . . ."
Then the two hapless young thieves have their backsides caned. It's
taken at a slow pace, the strokes laid on methodically, hard. It takes a
while, but they suffer 100 harsh, well-directed strokes apiece. As they
wriggle and writhe in agony, Sara lectures them grimly that: "You should
thank me for being so merciful. Because if you're ever caught thieving here
again, it'll be a bullwhip, right from the word 'go,' and I'll personally
see to it that you're whipped hard enough to take the skin off your backs,
hard enough that we put you in hospital." There's no doubt in anyone's mind
that she means it, and there's grim laughter when she adds: "And we'll
brand you, too."
The caning has added a dense pattern of welts to their already
bruised and striped skin, like basketwork. Sara looks at her watch and
smiles happily. Only 8pm. Plenty of time yet. Coffee and beer is served, at
the suggestion of one of the more motherly women. A brief break is taken,
with the crowd from the rows of overlooking seats coming down to look over
the two whipped women. Each gets a good inspection, but it's plain that
many of the crowd want a much more personal involvement, and are chatting
among themselves, ignoring them for now, awaiting that chance.
Now it's the guys' turn, Sara says, unless of course the young
ladies would prefer to continue with this particular treatment, instead . .
.? They shake their heads. That seems like enough permission to Sara, who
signals with a wave: "Okay. Time to fuck 'em."
Bib fronted jeans, coveralls and other elegant redneck clothes are
quickly discarded, along with ragged underwear that has seen better days.
These people aren't thieves, you see.
What a fine variety of penises: all shapes, colors and sizes. And
how unabashed these men are at exhibiting them to the two shoplifters, not
to mention the local women who might get the year-round benefits of the
better specimens, should they play their cards right. Neatly spreadeagled
like this, the two are quite well-positioned for the purposes most of the
men have in mind. A short folding ladder is left nearby, so anyone who
wants to put his penis in their mouths is catered for. The first couple of
men are delighted to find how tight Jenny is, the second of them even more
happy when it's discovered how tight her anus is. She's wriggling
delightfully as the ruffian slowly feeds his eight-inch cock into her, a
finger's breadth at a time. Laura, of course, is much sloppier, and various
vulgar displays are made of how loose she is, front and back. Squelch!
"Look at this fit in, then!"
"Well, look at this!" A coke bottle, various other objects, get to
undergo mysterious vanishings.
Sara is quite right when she says with a smirk: "I think little
Jenny probably sleeps with her fist in her girlfriend's cunt. But I bet she
finds it hard to get a finger in her own."
There's laughter and disbelief at this, but Sara insists: "Can't
you tell? They're a pair of lezzies, I'm sure. They're not like a classical
top-fem combination, but I think this little one gets to play the boy,
sometimes. Surprising her asshole wasn't looser, huh? Of course, it will be
from now on. . ."
It's amazing how much semen a gang of guys can produce! Oh, sure,
gallons, buckets, lakes in their own minds. But still a couple of liters,
anyway. And a little of this messy, smelly stuff goes a long way, when it's
spread around. The two are fucked, ass and pussy, and made to suck, jerked
off over, and generally hosed and smeared down until they look like they
have rolled in spunk, and shampooed with it. Enjoying themselves immensely,
the guys are in a socialist mood. There's a constant traffic to the
payphones out in the lobby to the gym, and several carloads of fresh guests
arrive, to be greeted with high signs, cheers of encouragement. Younger
brothers, some awestruck teens, barflies, two or three cops out of uniform,
older guys who are probably teachers.
Sloppy seconds, does anyone say? No, they're all too much into the
fun of the moment. The air is rich with funky smells. Several of the women
present have shed a lot of clothes too, to join in, in their own way. The
two victims endure three or four hours of non-stop, spirited fucking, until
they are hanging loosely in their bonds, beyond sobbing now, numbed. Nearly
midnight, and it might be getting time to go, since the heating system has
clicked off at 11pm and it's starting to get cooler.
Is there a suspicion that Jenny might actually have responded to
this mistreatment in some, how shall we say, positive way? It seems
possible. Remember, her panties were wet after she'd been shown, then
stripped. Sara had commented, privately, on her scent. And, tight though
she was, she hadn't been hurt or even made particularly sore by all the
fucking, even though they'd cruelly avoided greasing her first. No, she was
quite wet enough of her own accord. And overflowing enough to lubricate her
rear entrance, too.
Weeks after, guys comparing notes over beers, or reminiscing on the
phone will being saying: "You know, I think the little blackhaired one was
getting off on me. I could feel her cunt gripping me pretty hard . . . how
about you?"
"Yeah? I think she wanted to come, but didn't want the big blonde
cow getting jealous. She was dribbling. From the mouth, I mean. Oh, the
other end, too. She sure had a grip, boyo."
No one had thought to bring a video camera along, so the argument
raged on all summer. Instead of 'The one that got away,' the new version
was, 'Did she come or didn't she?'
And Laura? No one has any doubts, even some of the women who finger
and fistfuck her towards the end. "She could crush beercans with her twat,"
one of the boutique owners observed. "No doubt about whether she was
enjoying herself, in my mind."
Sara insists that the bedraggled, sore duo should kiss her feet
before they are allowed to leave, a homage several other storekeepers
decide had great appeal for them too.
When they're finally through mistreating the two women, the pair
are carried out shoulder-high, supported by a half-dozen men each, thrown
nude in the back of a waiting pick-up and driven to their motel. It's pitch
dark now, few streetlights on. They are helped to pack and taken naked,
standing in the back of the truck to the town line, where they find their
car has been parked, but decorated with suitable derisive comments.
Of the two, Jenny is the tougher, in the end. She will just be able
to drive, she agrees weakly. She's leaning on the car, trying to catch her
breath, her eyes staring flatly at the shocking, striped reflection of her
punished body that she now sees in its windows. So they give her the keys.
But they handcuff Laura, and chain and padlock her ankles. The keys
to those will be mailed to them at Bumherst, they're assured, "with some
wonderful photo souvenirs," in a day or two. "Keep her out of mischief till
then, you smelly little scumbag," Sara bids her farewell, leaning in the
driver's side window and bending close, bites Jenny's left nipple, hard,
grabbing the other with her nails. She chews, claws, then pulls back,
smilng happily. "And, hey, don't come back to Kittyquit if you have any
sense . . .Oh, and tell all your friends, too."
"Yeah, go shoplifting in New York next time, you cunts!" observant
Katie says happily.
Jenny gets the car started, anxious to be gone, waves feebly, and
pulls away. She drives slowly, asking Laura if she'll be okay, does she
need any help? Not right now. Jenny finds it hard to get comfortable on the
leather seats, her backside raw. how is she going to drive home naked? What
can she do when she gets there, to avoid being seen like this?
It's a subject she won't have to address for a while, it seems.
Just a mile or two further down the road, police chief O'Reilly is waiting.
He's sitting there, by the side of the road in his squad car, with all the
lights flashing. As the Acura approaches, he flags it down.
He knows about the marijuana from a search done while they were
being tormented. No mind games are played, he simply looks down at Jenny
and says: "My troopers say they found about $200 worth of grass in your
car. Well?"
To Jenny, this is the end. She breaks down and sobs, head on the
wheel. It takes her a while to recover. He leads them to the station in
town, part of the firehouse, a little office space that's convenient for
the various patrol duties associated with the beach in summer, but is not
staffed at night.
He opens up, turns on the lights, then leads the two naked girls
in, Jenny first, then the hobbling Laura.
"Coffee?" he asks, starting to make a pot.
"Sit if you want." They don't.
And ignoring Jenny's pleas, he tells them they're in serious
trouble, and assures them that if it ever comes to trial, no one's going to
believe a word they say about the pillory, the beatings, the gang bang. He
can find dozens of witnesses to counter any story: If anyone disbelieves,
they'll be character-assassinated as nymphos and lezzies, masochists and
freaks, who'd asked for it.
So why go through all that worry, just to prove a point? Especially
when they won't!
He has a simple proposition to make: sexual slavery at the summer
break, or jail. And what he's suggesting is a little trial run now, since
no one's expecting to see them back at Bumherst for a few days, right?
The two look dumbfounded, but see no choice. Give themselves to
this paunchy, middle-aged cop? Gross! But . . .
He wants Laura. Big, generously endowed woman appeal to him. But he
tells her: "I'll have to put you in the hot tub first. You smell like
everyone from here to New Hampshire has fucked you." He's not far wrong,
Jenny, he decides, is a stringbean. He'll give her to Sara as a
present, in the hopes of sparking some gratitude and getting into that
rather attractive woman's pants, at some stage in the future.
So, after the contract he's written is signed — it's a mere
one-pager, saying simply that the signatory consigns herself with no
questions into the hands of the bearer of the contract for sexual purposes
— he calls Sara. She's been chatting with the other woman and has only
just got home. His call catches her a little grouchy and surprised, since
she was just thinking about going to bed. He makes his pitch.
Ten minutes later, with a squeal of tires, she's there, breathing
heavily, eyes sparkling at his ingenuity. She's thrown an overcoat over her
nightie, driven down in slippers.
She accepts O'Reilly's offer with a big hug. "Four days?
Absolutely. I'll bring her back here. And no, I won't harm her any more
than I have already . . ."
She looks around the small office, and finds some plastic
disposable cuffs. She binds Jenny's wrists, ropes her ankles, then attaches
a piece of rope round Jenny's throat. She eagerly leads her away to her
truck . . .
Measuring her captive's huge purple clitoris next morning Sara
tells Jenny with a big smile: "You are a tasty little thing, but I could
tell that when I sniffed you. I like this big hairy mess, but I'm going to
shave your pussy lips and asshole to make it easier to get my dildo and my
fingers in. You're a real treat, my dear . . ."
It's all taking place with the girl bent back naked over a table on
Sara's sundeck, overlooking the scenic coastal walk. In summer, a place as
public as can be. She looks down at the ruler again. "Huge! You must spend
half your life wanking, my girl! I think hubby #2 was smaller in the panty
torpedo department, Jenny. Now, try to be nice. You know you lost your
inhibitions last night, but you mustn't pout all day. If being fucked by
another woman pleases you so much, then just resign yourself to it, and
accept it for what it is . . . I heard all that moaning when I sat on your
face! All that spunk didn't spoil your appetite for me, did it? So, lighten
up and don't be so resentful! It's not like I'll be able to beat you again
for a while. Well, today anyway, so long as you're a very obedient little
fuckslut, and do just as you're told . . ."
'The one that got away'? Sara knows all the answers. Including the
answer to the guys' speculation about whether Jenny came, and whether
having her ass thrashed excited her . . .she's just not saying, but I think
you already figured it out, didn't you?
[NB: I'd consider a sequel if I get enough response]
Disclaimer: Adults only, whatever that means wherever you are lucky enough
to be reading this. If you don't like [NC, humil, spanking] stories, this
isn't for you.
This item is of fictional nature. All persons and places in it are
imaginary and no resemblance to real or historic characters is intended. No
illicit behavior is endorsed or condoned. Art and/or Entertainment is the
*Copyright* is claimed, 1997 by Baton Rouge ThoughtScapes, and for
the author, Mr.Spraycan, who chooses to be 'anon'. For entertainment
purposes only. No commercial use is warranted without permission. Do not
repost. Store only with this notice intact.
This is MrSpraycan story No. 42
Magic word: "Feedback!"


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Directly and indirectly, the housing bust that began in 2007 put "the bailout" on the map in the lexicon of industrial policy both in Europe and North America. Whereas in the U.S., few restrictions were placed on the recipients, the E.U.'s first €37 billion ($47.9 billion) for Spain's banking sector required the four major state banks "to make sharp cuts in their balance sheets and payrolls," according to the Wall Street Journal. Bankia, the largest of the banks to be bailed out, planned to cut its number of employees by more than 6,000, close more than 1,000 branches, pass on any further real-estate lending, and reduce its assets by €50 billion as the bank focuses on retail banking—getting back to the knitting, as it were. Presumably the bankers were not allowed to grant themselves bonuses as a condition of the bailout. If so, it would differ appreciably from the U.S. bailout of Wall Street banks.
Avoiding the pitfalls of rewarding banks in trouble with black checks is one thing, however; seeing to it that the downsizing does not hamper the lending market itself is quite another. E.U. officials may have dropped the ball in this regard. At the time, banks based in Spain had a total of €1.7 trillion in outstanding loans, down from a peak of more than €1.8 trillion. A further fall of €300 billion was expected as a result of the cutbacks at the bailed-out banks.
The IMF, a technical adviser on the bailout, suggested that state officials in Spain "must make sure the downsizing . . . can be compensated by expanding lending by other banks." It is doubtful, however, that the beleaguered state was in any position at the time to stimulate lending by other banks—particularly given that monetary policy was set by the European Central Bank and Spain itself was incurring deficits amid a high unemployment rate. After all, the bailout funds were coming from the E.U. That the €37 billion was only the first piece of a €100 billion "rescue line of credit for Spanish banks" suggests that more could have been done by the E.U. to encourage smaller, more stable banks in Spain to increase their lending. That in turn could have expanded economic output in the state—a worthy goal given that people under 25 faced a 50% unemployment rate at the time in Spain.
Likewise, the TARP program in the U.S. could have given just enough to the big banks to keep them from following Lehman Brothers (i.e., to stop the conflation enabled by short-sellers), while demanding that those banks downsize. At the very least, being too big to fail would have been reason enough for that policy (plus a cap on bonuses). The disbursement of TARP funds to smaller banks sufficiently solid financially that the funds could serve as a fertilizer of sorts for reasonable (not explosive) growth could have facilitated a basic shift. That is to say, the biggies could have been contracting as the "next generation" was being helped along to take up the slack. The resulting credit in the economy would have acted as a stimulus to economic activity (hence reducing unemployment).
In short, "bailout" could have been viewed both in the E.U. and U.S. as only part of the equation to be applied to trouble-spots in the economy. By analogy, a person can facilitate weight-loss by adding an exercise regime (and a stimulating diet of fruits and vegetables) to cutting the total number of calories ingested. A viable industrial policy favors bright spots while managing problem-children in such a way that their activity is restricted rather than rewarded. This may be one of the principal lessons coming out of the housing bust that began in 2007.
Christopher Bjork and Vanessa Mock, "EU Clears Spanish Bank Rescue," The Wall Street Journal, November 29, 2012.


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Handpainted Snakes & Ladders
Rs. 1,195.00
Partners SUKRITI INPRINT SKU: VL/64/1
|Ready stock. It can be delivered within 3-5 days. Returns accepted
|Length - 12 Inches, Width - 12 Inches Height 1.3 Inches.
|820 grams. (approx)
|A handpainted cardboard "Snakes & Ladders" board with handmade gotees /tokens in the shape of different colored cute tiny turtles. Comes with a leaflet for playing instructions.
|A soft cotton cloth when needed. Keep away from perfumes, sprays, water and other liquid substances.
|Slight difference in color from the visible product image is possible. Read more.
Sukriti In print was founded in 1979. For over three decades we have been designing and manufacturing beautiful stationery and gift products. Our products take inspiration from India's rich art, craft and textile traditions. Our products are hand made by master artisans who maintain careful personal attention to detail. They are highly skilled in a variety of bookbinding and handmade stationery manufacturing techniques.


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It's going to be another amazing year at the Kids' Choice Awards, with Blake Shelton serving as the host of the evening.
And this morning (February 2) a partial list of nominees went live, including the hopefuls for Favorite Movie, Favorite Talent Competition Show, Favorite Movie Actor and Favorite Movie Actress.
He's no stranger to the #1 spot and Tom Cruise took his latest flick "Mission: Impossible- Rogue Nation" to the top of the rankings for its opening weekend.
The action-packed film, also starring Jeremy Renner, Simon Pegg and Rebecca Ferguson, raked in $56 million, followed by "Vacation" with $14.9 million in earnings.
Despite the relatively low attendance at movie theaters across the nation this past weekend, "Ant-Man" still managed to rake in enough ticket sales to remain in the top spot.
The Paul Rudd flick landed $24.8 million, putting it just ahead of Adam Sandler's opener "Pixels," which earned a respectable $24 million.
It was a respectable opening weekend for Paul Rudd's latest flick "Ant Man," as moviegoers bought $57.3 million worth of tickets to land it at number one.
The Marvel/Disney film managed to beat last week's top dog "Minions," which raked in an additional $50.2 million and wound up in second place.
Besides fireworks and barbecue gatherings, July 4th weekend is also known for plenty of trips to the movie theaters across the nation.
And over the past few days, "Jurassic World" proved to be the dominating force yet again, bringing in $30.9 million to total more than $558 million domestically.
Similar to the way dinosaurs dominated the earth millions of years ago, "Jurassic World" just keeps ruling movie theaters nationwide.
The Chris Pratt flick was #1 for the third weekend in a row, raking in a handsome $54.2 million, while uber-popular animated movie "Inside Out" came in at a close 2nd with $52.1 million.
Up until this past weekend, "The Avengers" had the title for highest-earning opening weekend, but now "Jurassic World" is the reigning champion.
After tallying up the total domestic sales figures, the Chris Pratt flick surpassed the Marvel films' $207.4 million with $208.8 million, and when added to its $315.3 million international take, "Jurassic World" is also the biggest global opener of all time.
Everyone knew that "Jurassic World" would be a huge flick, but over the weekend it raked in a global box office total of $511.8 million!
The Chris Pratt project scored $204.6 million domestically, putting it at #1 for June openings and #2 for all-time openings, just behind "The Avengers" which earned $207.4 million.


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Workflow TV - State Machine Tutorial
Download this episode
In this video Steve Danielson, a Programming Writer on the WF team, gives a walkthrough of the WF4 State Machine Getting Started Tutorial from the MSDN documentation.
- Getting Started Tutorial
- Completed Version of the WF4 Getting Started Tutorial
- .NET Framework 4 Platform Update 1 Overview
- Windows Workflow Foundation (WF4) - Introduction to State Machine Hands On Lab
Available formats for this video:
Actual format may change based on video formats available and browser capability.
More episodes in this series
Workflow TV - How to create a Custom Activity Designer
Related episodes
Workflow TV - Three Simple Rules for Better Debugging with WorkflowApplication
Workflow TV - Tracking StateMachines
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text:
|Yan, Liuling
|Fu, Daolin
|Li, Chengxia
|Blechl, Ann
|Tranquilli, Gabriela
|Bonafede, Marcos
|Sanchez, Alexandra
|Valarik, Miroslav
|Yasuda, Shozo
|Dubcovsky, Jorge
Submitted to: Proceedings of the National Academy of Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/3/2006
Publication Date: 12/19/2006
Citation: Yan, L., Fu, D., Li, C., Blechl, A.E., Tranquilli, G., Bonafede, M., Sanchez, A.N., Valarik, M., Yasuda, S., Dubcovsky, J. 2006. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proceedings of the National Academy of Sciences. 103: 19581-19586 Interpretive Summary: Wheat and barley are cereal crops grown in many different temperate environments worldwide. "Winter" wheat and barley varieties require extended periods of exposure to low temperatures to accelerate flowering (vernalization), whereas "spring" varieties do not have this requirement. The research in this paper was aimed at isolating and sequencing a gene called Vernalization 3 or VRN3 that determines whether a variety requires vernalization for synchronous efficient flowering, i.e. whether a variety is a spring or a winter wheat. The VRN3 gene encodes a signal molecule that promotes flowering. When it is functional, the plant does not require vernalization. Winter varieties carry non-functional mutations in this gene and thus require vernlization to activate the flowering pathway. Increasing expression of VRN3 in a winter wheat by genetic transformation accelerated flowering by more than 60 days. The isolation and characterization of this gene gives breeders a new tool with which they can vary heading times and ensure optimum flowering for different wheat and barley varieties grown under different climate conditions.
Technical Abstract: Winter wheat and barley varieties require an extended exposure to low temperatures to accelerate flowering (vernalization), whereas spring varieties do not have this requirement. In this study, we show that in these species the vernalization gene VRN3 is completely linked to a gene similar to Arabidopsis FLOWERING LOCUS T (FT). FT induction in the leaves results in a transmissible signal that promotes flowering. Transcript levels of the barley and wheat orthologues, designated as HvFT and TaFT respectively, are significantly higher in plants homozygous for the dominant Vrn3 alleles (early flowering) than in plants homozygous for the recessive vrn3 alleles (late flowering). In wheat, the dominant Vrn3 allele is associated with the insertion of a retroelement in the TaFT promoter, whereas in barley, mutations in the HvFT first intron differentiate plants with dominant and recessive VRN3 alleles. Winter wheat plants transformed with the TaFT allele carrying the promoter retroelement insertion flowered significantly earlier than non-transgenic plants, supporting the identity between TaFT and VRN-B3. Statistical analyses of flowering times confirmed the presence of significant interactions between vernalization and FT allelic classes in both wheat and barley (P<0.0001). These interactions were further supported by the observed up-regulation of HvFT transcript levels by vernalization in barley winter plants (P=0.002). These results confirmed that the wheat and barley FT genes are responsible for natural allelic variation in vernalization requirement, providing additional sources of adaptive diversity to these economically important crops.


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