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1Department of Neuroscience, Thomas Jefferson University Medical College
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Neural precursor transplantation is a promising strategy for protecting and/or replacing lost/dysfunctional cervical phrenic motor neurons in spinal cord injury (SCI) and the motor neuron disorder, amyotrophic laterals sclerosis (ALS). We provide a protocol for cell delivery to cervical spinal cord ventral horn in rodent models of ALS and SCI.
Date Published: 9/18/2011, Issue 55; doi: 10.3791/3069
Keywords: Medicine, Issue 55, cell transplantation, engraftment, graft, spinal cord, stem cells, precursors, ALS, amyotrophic lateral sclerosis, motor neuron, SCI, spinal cord injury
Lepore, A. C. Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury. J. Vis. Exp. (55), e3069, doi:10.3791/3069 (2011).
Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) cases 1 and is the ultimate cause of death in patients with the motor neuron disorder, amyotrophic laterals sclerosis (ALS) 2.
ALS is a devastating neurological disorder that is characterized by relatively rapid degeneration of upper and lower motor neurons. Patients ultimately succumb to the disease on average 2-5 years following diagnosis because of respiratory paralysis due to loss of phrenic motor neuron innnervation of the diaphragm 3. The vast majority of cases are sporadic, while 10% are of the familial form. Approximately twenty percent of familial cases are linked to various point mutations in the Cu/Zn superoxide dismutase 1 (SOD1) gene on chromosome 21 4. Transgenic mice 4,5 and rats 6 carrying mutant human SOD1 genes (G93A, G37R, G86R, G85R) have been generated, and, despite the existence of other animal models of motor neuron loss, are currently the most highly used models of the disease.
Spinal cord injury (SCI) is a heterogeneous set of conditions resulting from physical trauma to the spinal cord, with functional outcome varying according to the type, location and severity of the injury 7. Nevertheless, approximately half of human SCI cases affect cervical regions, resulting in debilitating respiratory dysfunction due to phrenic motor neuron loss and injury to descending bulbospinal respiratory axons 1. A number of animal models of SCI have been developed, with the most commonly used and clinically-relevant being the contusion 8.
Transplantation of various classes of neural precursor cells (NPCs) is a promising therapeutic strategy for treatment of traumatic CNS injuries and neurodegeneration, including ALS and SCI, because of the ability to replace lost or dysfunctional CNS cell types, provide neuroprotection, and deliver gene factors of interest 9.
Animal models of both ALS and SCI can model many clinically-relevant aspects of these diseases, including phrenic motor neuron loss and consequent respiratory compromise 10,11. In order to evaluate the efficacy of NPC-based strategies on respiratory function in these animal models of ALS and SCI, cellular interventions must be specifically directed to regions containing therapeutically relevant targets such as phrenic motor neurons. We provide a detailed protocol for multi-segmental, intraspinal transplantation of NPCs into the cervical spinal cord ventral gray matter of neurodegenerative models such as SOD1G93A mice and rats, as well as spinal cord injured rats and mice 11.
1. Cell Preparation
As an example, we will describe the procedure for preparing glial progenitor cells 12 for transplantation because of our experience with this cell type. However, the specifics of the protocol, including medium and use of trypsin for example, will depend on the particular cell type being used for transplantation.
Optional: Add 5.0 mL/T-75 flask of 1.0 mg/ml soybean trypsin inhibitor in DMEM/F12.
Do not use 0.75 mL tubes, as the Hamilton syringe/needle cannot fit deeply into this tube. Multiple injections will likely be made during the surgery sessions, and one wants to avoid disturbing the same tube of cells many times. Try to prepare at least 50% more volume of cell suspension than is needed. Keep cells on ice throughout the surgery session. Transplant cells within 4-5 hours of preparing cell suspensions in order to assure greatest viability of cells post-transplantation.
2. Preparation Prior to Surgery
3. Surgery: Animal Preparation and Surgery
Animal Prep:
Troubleshooting Tips for Problems Associated with Various Steps of Protocol
Lack of/poor cell survival: This is likely not a technical issue associated with the injection, but is probably due to properties of the cell type being injected and/or to the immunesuppression regimen. These issues need to be empirically evaluated on a cell-type and animal model specific basis. The availability of a number of immune-deficient rat and mouse models are also available to circumvent problems with immunesuppression; however, these animals also present difficulties such as cost, the need for maintaining a colony, and additional caution necessary during surgery and housing.
Functional deficits observed following surgery: In our experience, we have not observed the occurrence of any functional deficits following this procedure when assessed by measures such as forelimb and hindlimb grip strength and phrenic nerve/diaphragm compounds muscle action potentials, even with 6 injections sites (of 2μL each) in the cervical spinal cord. Tissue destruction has also not been observed. Possible reasons for the occurrence of these problems include: not using the suggested needle gauge, injecting larger volumes and/or cell numbers, unintentional damage caused by improper laminectomy, lack of delicacy while cutting the dura (such as pinching the cord with the #5 forceps) or during the process of inserting the Hamilton needle into the spinal cord (replace a dull needle).
Cells not found in ventral gray matter: This could be due to a number of issues. If the injection misses the target medially or laterally, make sure to properly line up the injection system parallel with the axis of the spinal cord, and insert the needle just medial to the entry of the dorsal rootlets. If the injection misses the target dorsally or ventrally, make sure to zero your z-axis reading when the tip of the needle is just barely touching the dorsal surface of the spinal cord, use the suggested Hamilton needle with the appropriate bevel (a longer bevel can affect targeting), assure that the spinal cord does not compress when inserting the needle, take care in exactly measuring depth using the micromanipulator, and use animals within the suggested weight range. Adjust your technique accordingly if you are consistently injecting the cells in a location (which requires histological evaluation and subsequent technique modification). If the injections are randomly scattered at all of your injection sites, you will need to practice to improve consistency. If the cells tend to be located mostly at the dorsal aspect of the spinal cord along the needle track, wait longer before and after cell injection, and extend the actual injection over a longer period of time.
4. Representative Results:
Mouse-derived glial progenitor cells were transplanted (50,000 cells/site) into the ventral horn of spinal cord level C4 in an adult SOD1G93A rat. Mouse-derived transplanted cells can be distinguished from host rat tissue via detection with the mouse-specific antibody, M2. This image shows the survival of M2+ transplant-derived cells at 1 month post-transplantation (see Figure 12). The cells localized to the ventral gray matter, but the injection site medially missed the lateral ventral horn (the location of most phrenic motor neurons: denoted by dotted line). No cyst formation was seen when 50,000 cells were injected per site, and no behavioral impairment resulted from the injection procedure. However, injection of much higher numbers of cells (the actual number varies according to cell type, and should be systematically evaluated) results in damage at the injection site and along the needle track (see Figure 13: immunohistochemistry with the astrocyte marker, GFAP).
Figure 1. Initial incision of skin.On the lowest microscope magnification (we use 8 x magnification), use scalpel blade to make midline incision. Stretch skin laterally with other hand to make skin taut (which makes the skin easier to cut), and make incision (denoted by thick dotted line)from base of skull (at the level of the back of the ears) to shoulder blade (denoted by thin dotted line).
Figure 2. Exposure of surgical field.The surgical exposure should be square/rectangle-shaped. This shape can be achieved by pulling the surrounding muscle towards 4 corners using the 4 retractors. Tape string that is attached to retractors to surgical board in order to secure retractors and properly keep field open.
Figure 2-inset. Retractors for exposure of surgical field.The retractors are used to pull back muscle in order to create a surgical field with both clear visibility of the spinal cord and adequate space to perform surgery. Retractors can be made by shaping sturdy paperclips into the desired shape and size. Autoclave the retractors before for surgery. Tie string to retractor.
Figure 3. Vertebrae. Following removal of paraspinal muscle, thoroughly clean dorsal surface of vertebrae with rongeur. Individual lamina can be seen, as well as dorsal rootlets entering from lateral aspect of spinal column.
Figure 4. Laminectomy.Start laminectomy at spinal cord level C5. Secure spinal column by holding muscle overlying level C2 with rat toothed forceps. Grab entire lamina (see diagram: grab near midline) with rongeur. Position rongeur so that tool is completely perpendicular to axis of spinal column. Slowly crush lamina. Do not push down into spinal cord, as this will cause damage to spinal tissue. Crush and gently pull broken piece of bone upwards. Rongeur should crush piece so that one can easily pull away to remove. If piece of bone is still attached to rest of laminae, do not tug as this will cause hemorrhage and possible injury to spinal cord. Rongeur should be clean and sharp.
Figure 5. Exposure of spinal cord tissue following laminectomy.Extend laminectomy to expose all of C4-C6 spinal cord. Make 1 continuous opening in the bone over 3 spinal levels. Do not extend laminectomy too far laterally because this will cause hemorrhage. In order to target ventral horn, the injection site is relatively medial, so it is unnecessary to extend laminectomy to the complete lateral extent of the vertebral bone.
Figure 6. High magnification of dorsal surface of spinal cord.The prominent dorsal blood vessel can be seen running down the midline of the spinal cord. This blood vessel pattern is observed in most cases; however, some animals display a non-midline trajectory of the blood vessel. The dorsal rootlets can be seen at the lateral aspects of the spinal cord dorsal surface. Relative to the dura overlying the spinal cord, these nerves have a hazy appearance.
Figure 7. Injection setup.Line up syringe/needle parallel with axis of spinal column to properly target desired anatomical region. The needle is angled just enough (approximately 80-degrees relative to the surgical table) to not bump the surgical scope head, but as close to 90-degrees as possible (left panel).Lower injection tip towards the spinal cord dorsal surface using the microscope (right panel). Gently touch surface of spinal cord with tip of needle. Slightly depress cord with needle. Retract needle until spinal cord is back to normal flat state. Record this position as z = 0.0 using the ruler on the micromanipulator.
Figure 8. High magnification of spinal cord injection Aim needle just medial to the entry zone of the dorsal rootlets (denoted by dotted line).
Figure 9. Diagram of spinal cord: how to target anatomical region of interest. When attempting to target the ventral horn, incise dura parallel to axis of spinal column just medial to the entry zone of the dorsal rootlets. This will allow one to target the ventral horn. Lower needle to depth of 1.5 mm to target ventral horn in adult rats (the age and sex of the animal does not make much of difference on depth). Lower needle to depth of 0.75 mm to target ventral horn in adult mice. Of course, depth and lateral position depend on the specific region of interest.
Figure 10. Closure of surgical site.Suture closed three overlying muscle layers at one time with 4-0 suture. Suture muscles at 3 locations in rostral-caudal axis.
Figure 11. Stapling of skin.Staple skin closed with 9.0 mm wound clips. Tighten staples with needle holders to prevent the animal from pulling off staples prior to full wound healing. Space staples approximately 0.5 mm apart.
Figure 12. Transplantation of glial progenitors into cervical ventral horn. 50,000 mouse-derived glial progenitor cells were transplanted into the ventral horn of spinal cord level C4 in an SOD1G93A rat. M2+ mouse-derived transplanted cells survived at 1 month post-transplantation. The cells localized to the ventral gray matter, but the injection site medially missed the lateral ventral horn (denoted by dotted line).
Figure 13. Tissue injury following transplantation of high numbers of neural precursor cells. Injection of much higher numbers of cells (the actual number varies according to cell type, and should be systematically evaluated) results in damage at the injection site and along the needle track.
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For studies involving SOD1G93A mice and rats, age- and sex-match the animals within a group, and distribute animals within the same litter to different groups. It is preferable to use all animals from the same sex for both ALS and SCI models because disease processes may differ between males and females; however, it may also be useful to have enough animals from both sexes to detect possible sex-specific effects, as this phenomenon has been reported with SOD1G93A rats 13 and SOD1G93A mice 14,15. Consider the age/point of disease (for ALS animals) or time post-injury (for SCI models) for transplantation of cells into animals. This strategy will likely differ depending on the specific cell mechanisms that are being addressed.
The protocol described here can be used for both rat and mouse cervical spinal cords 11, as well as for transplantation into other levels of the rat and mouse spinal cords such as thoracic and lumbar levels 14. In order to target specific anatomical structures (other than ventral horn) in these various spinal cord levels, it is advisable to first test the medial/lateral position and depth of injections using a dye or some other means to evaluate the accuracy of the coordinates.
Outcome measures include detailed analysis of cell transplant fate, biochemical, histological, and functional states of the spinal cord, and behavioral changes relevant to the location of transplantation. The specifics of behavior testing, histological analysis and other outcome measures analyzed following transplantation should be tailored to the experimental needs/model.
Having a surgical assistant to anesthetize, shave, "open" and "close" the animals is quite helpful for smoothly getting through the most animals as possible. In addition, a surgical assistant can help the surgeon to remain sterile throughout the procedure.
The optimal number and concentration of cells injected will vary depending on the size of the cells, the species of the recipient animal, and the disease condition. For example, compared to the intact spinal cord, increased numbers of cells can be delivered into a SCI site if a cavity has already formed 16. However, the specific parameters need to be systematically optimized prior to undertaking a large study.
Prior to conducting a large study with a specific NPC type, evaluate the long-term effectiveness of the immune suppression regimen. In addition, cell survival may vary for a given cell type depending on the disease model or the timing and location of transplantation.
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No conflicts of interest declared.
I would like to thank: all members of the Lepore, Maragakis and Rothstein labs for helpful discussion; The Paralyzed Veterans of America and the Craig H. Neilsen Foundation for funding.
|Catalog Number
|GIBCO, by Life Technologies
|0.05% Trypsin
|GIBCO, by Life Technologies
|Soybean Trypsin Inhibitor (optional)
|Acepromazine maleate (0.7 mg/kg)
|Fermenta Animal Health
|Ketamine (95 mg/kg)
|Fort Dodge Animal Health
|Xylazine (10 mg/kg)
|Bayer AG
|#11 Feather surgical blade
|Electron Microscopy Sciences
|Cotton-tipped applicators (6 inch)
|Fisher Scientific
|Rat-toothed forceps
|Fine Science Tools
|Rat: 11023-15; Mouse: 11042-08
|Medium-sized spring scissors
|Fine Science Tools
|Mini spring scissors
|Fine Science Tools
|Fine Science Tools
|Rat: 16121-14; Mouse: 16221-14
|Fine Science Tools
|Needle holders
|Fine Science Tools
|Suture: 4-0
|Staples: 9 mm
|Stapler: 9 mm (Reflex #203-1000)
|World Precision Instruments, Inc.
|Warm water pump (T/Pump)
|Gaymar Industries
|Cyclosporin A: 250.0 mg/5.0 mL ampules
|Novartis AG
|LC Laboratories
|LC Laboratories
|World Precision Instruments, Inc.
|Micro 4 Microsyringe Pump Controller
|World Precision Instruments, Inc.
|World Precision Instruments, Inc.
|10.0 μL Hamilton syringe
|Hamilton Co
|Hamilton needles: 33-gauge, 45° bevel, 1 inch
|Hamilton Co
|Glass 20.0 μL microcapillary pipettes (optional)
|Kimble Chase
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Astronautics Vocabulary
The Astronautics Vocabulary is a glossary of terms that pertain to the science and technology of spaceflight. This alphabetical list can be navigated by clicking on the letters A-Z displayed on this page.
Definition of pitch
Translation: tangage
Definition: Of an aerospace vehicle, an angular displacement about an axis parallel to the lateral axis of the vehicle.
Other Definition: None
Synonym: None
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subset: commoncrawl


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Sunday, March 25, 2012
Most Ridiculous HuffPo Bias Yet
2 comments:
Of course the HuffPo won't tell the truth which has been reported in the Israeli media. It is HAMAS which has refused oil shipments, and diesel shipments which the Israelis were willing to transfer. This is an energy shortage which has been engineered to kill Arabs BY THEIR DEMOCRATICALLY ELECTED GOVERNMENT. If, in fact, this baby died, it is entirely the fault of the Arabs.ReplyDelete
They want dead Arab children to try to smear the Israelis with. So they dig up years' old pictures of children who died in accidents and pretend that they are new "victims" of the Jews, and they kill their own babies for the same reason.
The whole society is sick.
"Honest Reporting" today notes that AP retracted this story BECAUSE IT'S A LIE.ReplyDelete
Hey guys we've started to employ a slight comment policy. We used to have completely open comments but then people abused it. So our comment policy is such: No obvious trolling or spamming. And be warned: unlike the Huffington Post we actually enforce our comment policy.


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Today, Congressman Sanford D. Bishop, Jr. (GA-02) released the following statement after the dedication ceremony for the Rosa Parks statue in the U.S. Capitol.
"Rosa Parks' conscientious decision to continue to sit inspired others to stand up in nonviolent resistance against oppression and opened the door for not only those of us who have been able to emerge as leaders in her wake, but for all Americans who now know greater freedom thanks to her sacrifice," said Congressman Bishop. "Her statue in the U.S. Capitol will be a lasting reminder of the ideals which she exemplified and we hold so dear. Rosa Parks proved to our nation and to the world that, no matter how small, one can make a difference."
The new statue of Rosa Parks is the first full-size statue of an African-American woman in the U.S. Capitol. Congressman Bishop joined President Barack Obama and his colleagues from the House and the Senate at today's dedication.
Source: http://bishop.house.gov/press-release/bishop-statement-dedication-rosa-parks-statue


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She's been having a blast with her daytime hit "The Tyra Show" for the last five seasons, but now Tyra Banks has said she's moving on.
The Victoria's Secret vixen announced, "This will be the last season of The Tyra Show. I've been loving having fun, coming into your living rooms, bedrooms, hair salons for the past five years."
Her hard work is paying off, as Tyra Banks has been named the top moneymaking female on prime time TV.
Thanks to her hit show, "America's Next Top Model," Banks has scored the number one spot on the Forbes list of top earning women on prime time, beating out stars like Eva Longoria, Katherine Heigl and Julia Louis Dreyfus.
Getting back to her modeling roots, Tyra Banks was spotted doing a photo shoot in New York City earlier today (October 12).
The "America's Next Top Model" diva looked amazing as she donned a variety of outfits and poses while the photographer snapped up plenty of sexy pics.
She's quite savvy when it comes to her career and earlier today (August 19), Tyra Banks was hard at work on the set of her show in Chelsea, New York.
The 35-year-old model taped an outdoor segment for "The Tyra Banks Show" and gave some lucky members of the audience a chance to win some cash.
Hard at work on the set, Tyra Banks was spotted on location in Union Square for "The Tyra Banks Show" on Monday (August 17).
The model beauty is currently gearing up for the Season 5 premiere of her popular series, which kicks off on September 8th on the CW.
We're used to seeing her on the talk show scene, or judging "America's Next Top Model" but today (August 6) Tyra Banks was spotted on the "Gossip Girl" set.
The stunning supermodel looked lovely as she exited her trailer and strolled over to the Midtown Manhattan set, sporting a black bathrobe.
She's a seasoned veteran in front of the camera, and yesterday (July 30) Tyra Banks was spotted showing off her penchant for posing.
The "America's Next Top Model" host donned a series of sexy outfits and struck a plethora of poses during a photo shoot in the Soho district of Manhattan.
Offering a little relief to the catwalk queen, the alleged stalker of Tyra Banks has been convicted of multiple charges including stalking, harassment, attempted aggravated harassment, and criminal trespass earlier this morning (April 30).
With Tyra testifying at Manhattan's criminal court just yesterday, Brady Green will hopefully no longer be a problem for Miss Banks.


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Horns and turn signals
Hey all, two questions...
Any recommendations on replacement horns? Stock work just want it a little louder. I used to drive fire trucks and bumper mounted air horns would be awesome, but I need something a little more practical...
I've noticed my left turn signal will stay on when activated, not blink. Resetting the turn signal lever will make it work sometimes. Also, I've had it light up (on the dash) when applying the brakes, but just the left. And there is no rhyme or reason as too when it happens. Short? Switch problem? Gremlins?
I am having the same problem. I believe it is the Turn Signal Switch. From what I've read it's a pretty cheap and easy fix, I've just been to lazy to do it for myself. The switch costs vary depending on the year. Hope this helps.
Going through the same problem with my left turn signal. Is constantly lit up and fails to function when you flick the switch, however, randomly if I turn on all the lights they will all turn on at first, but as soon as I hit the switch the left side lights go off (front markers). I am going to look for a DIY instructions for the replacement of the turn signal switch and will post if I find anything.
Here we go: http://www.wranglerforum.com/f5/tj-t...der-41336.html
This forum rocks.
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BAVSOUND Mobile Electronics Forum
Have all your A/V and electronics questions answered here. Ipods, A/V, Radar Detectors Oh My!
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Join Date: Feb 2012
Location: Boise ID
Posts: 56
My Ride: '01 330i '06 M3
My experiences with the Dynavin 99+ and Enco 146
Now that I have both the Enco 146 (WinCE) and the new Dynavin 99+ I thought that it might help to do a quick comparison... I have the Enco in my 330i and the Dynavin in the M3.
Enco unit...
Although the initial cost is cheap, the TCO may not be worth it for some. If you have a problem with the unit you will have to deal with the manufacturer in China. And in a majority of the documented encounters haven't inspired any real confidence in getting problems resolved in a timely or satisfactory manner. Knock on wood that my unit just exhibits the "normal" Enco "features" .
Now for the Enco features.
The radio side's volume is completely out of sync with the GPS side. GPS, discs, bluetooth all require normal volume settings (around 20), but swith to the radio and watch out... A setting of anything more than 3 will burst your eardrums. The "fix" is to set the equalizer to Jazz and then it comes closer to the norm.
Every time you start the car (and the HU) the HU randomly picks a different source. So just listening to the radio is not a set it and forget it feature... I found hitting the mode button will set the source to radio (most of the time).
The unit occasionally rewrites startup files on the GPS SD card and changes the look of how the GPS starts and runs... And don't hit the red power button on the screen. You'll have to remove power from the unit to get the GPS back.
The WinCE version is Chinese... Do NOT hit Yes to "Do you want to revert back to system defaults" unless you're up on you Chinese...
And my unit has reverted back to the (what I call) the single source mode. The unit now starts up to the radio, but to change sources, one must go back to the main menu and select the GPS or other source. And it will not respond to any attempt to turn the unit off. It eventually goes back to the normal random mode... When you get tired of this it's hard reset time again...
So it's a cheap HU that will do most of what's expected of it, but too many issues for me to consider ever purchasing another... The unit does work, but the nits really detract from the user's enjoyment...
Hence the Dynavin in the M3... I was pleasantly surprised to find tha the WinCE version of the D99+ was less expensive than the original. Still more than the Enco, but one has to consider what their time and aggravation is worth. I ordered it from J&T (Jeff)... The unit got here before the car did!!!
The look is the same and the installation is the same, but the Dynavin is leaps and bounds ahead of the Enco. I had an "issue" with the radio side... I emailed Jeff and he had me disable a setting that I shouldn't have enabled and Badda-boom bada-bing tit was working as advertised...
Speed in getting the unit... J&T win...
Customer support... Goes to the J&T win column.
Volume... No matter what the source... The volume is NORMAL! Whoo hoo... I'm almost giddy with joy... Dynavin...
The interface is better IMHO... And access to system setting are easier and far more complete.
The GPS s/w the same (iGo Primo) so that's a wash (kinda) assuming that you've downloaded it to you Enco. Which happened to be a royal PITA. It's a licensed version with the Dynavin and you get the added benefits that you won't get with an unlicensed version.
So, the win goes to the Dynavin. Yeah it costs a bit more, but it works like it's supposed to. I don't have to constantly punch buttons just to get the radio... And I'm betting that if I have a problem with the Dynavin, it will be a lot easier to get resolved with Jeff than with our friends in the Far East... And cost less too.
It's all in what you want. If you want an transparent install and usage, get the Dynavin... If you like to constantly fiddle around with setting and trying guess what the unit will do next and love challenges, then get the Enco.
If I sound like a Dynavin fanboy, so be it... I think that the Dynavin is a better value even if the Enco is cheaper...
'08 Kawasaki Concours C14
Last edited by r2t2; 02-04-2013 at 05:08 PM.
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Cool story.
Registered User
Join Date: Mar 2007
Location: North NJ
Posts: 65
My Ride: 05 M3 ZCP no sunroof
I see that Parrot does the BT on the 99 and 99+. Is this solely for the adress book or does the Dynavin use the dual (noice cancelling) microphone and software as found in the Parrot MKI series. I really like my Parrot MKI9000 and people I am speaking to do not even know I am driving most of the time. The Parrot also has very good voice recognition and voice calling and answering which is very important to me since I do not have steering wheel controls on my ZCP.
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Location: Fresno, CA
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My Ride: BMW
thanks for posing this, unfortunately there are too many people who are under the impression that the knock off units are "clones" and think they are saving money by going the China Direct route.....as you found, they are NOT the same.....and you get what you pay for. I think the threads on this forum speak for themselves but its good to have some 'side by side' comparisons.
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Our classrooms and conference rooms contain moveable, reconfigurable furniture and are well suited to discussions, lectures and meetings for groups ranging in size from 8 to 100 people.
All classrooms are equipped with AV technology including projection, projection input, and network jacks. Additional technology may be requested.
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text: Drug addiction is a chronic disease. It does not discriminate against gender, race or age. Seniors are at great risk because they account for one-third of all medications prescribed by doctors. It's known as the hidden epidemic in our state. Health experts say senior drug addiction is all too often misunderstood or even mis-diagnosed. And, anyone is vulnerable. Joy Hollowell continues her special report on how easy it is for elderly Mainers to become addicted. ===="Jonathan was my twin brother. I'm an identical twin. And unfortunately he passed away in '93 of AIDS."Scott Davis was in his third year of medical residency at the time. "And rather than deal with the loss," says Davis, "the grief, those issues when he died, I turned to narcotics." Davis says being a doctor helped hide his addiction. "I even had providers who would say, 'Well, you're a professional, you're a doctor, you're educated, you know what you're doing, here. Let me give you a month's worth of Vicoden with five refills," says Davis. Eventually, even that didn't work. "It was probably four to five years in my disease of addiction," says Davis, "and at one point, to be honest here, I had written a prescription that I shouldn't have, for myself." Davis has since written a book about his journey to recovery And he's taken on addiction in his professional life, as an Addiction Treatment Specialist at Penobscot Community Health Care in Bangor. "Individuals very quickly develop a tolerance to these drugs, with the opiates. Within three to four weeks, even if someone is taking the medicine as prescribed," says Dr. Davis. "The problem that with seniors as well is that as we get older, our bodies react differently to the metabolism of drugs." "Sometimes, these family members who are just trying to be helpful to our family, our loved ones, and we think we're doing the right thing by changing their medications, that's stuff that we absolutely do not recommend," says Penobscot County Chief Deputy Troy Morton. Morton and other law enforcement work closely with the medical community, to make sure seniors as well as their care givers, are aware of the dangers of prescription drugs. "From our experiences, there's really no age limit," says Chief Deputy Morton. "We've seen young teens involved and we've also seen our seniors involved." So how do you know when a senior is battling addiction?"One red flag is certainly- how much are they taking?" says Davis. "Did they start taking one to two and now they're taking four to six at a time?" Changes in behavior, excuses as to why they're taking so many pills, or even hiding their medications are also tip offs. Dr. Davis says previous alcohol or substance abuse can be triggers as well. "It is important not to panic," says Dr. Davis. "And to understand that there is very successful treatment out there."===There is help available for seniors, care givers or family members who are concerned about addiction.Office of Substance Abuse Information and Resource Center 1-800-499-0027 http://www.maineosa.org Emergency Hotline 1-888-568-1112Eastern Area Agency on Aging 1-800-432-7812


text:
Architecture Meets Intelligence in Black Sea Region!
Northern part of Turkey lying through the sea is called the Black Sea (Karadeniz) Region. This is the greenest region of Turkey, with a characteristic nature, architecture and daily life. The mountains are situated parallel to the shore, which affects the whole life of the people here, as well as the climate, agriculture, city planning and transportation.
The local people of Black Sea Region are famous for their stunning ability of solving problems. Especially, when there is a structural difficulty, because of the tough geographical conditions, people find really interesting solutions and they even build their own houses. Most of the interesting examples of Black Sea architecture are born in the city called Rize. Here are some examples of the functional design ideas:
Separating Heritage House: Two brothers from Rize could not agree on the heritage house after their father's death; so they just decided to divide the 120 sq meter house into two. One of the brothers demolished his part of old house, and built a 3-storey building. The other half of the old house is still being used as a woodshed by the other inheritor.
8 Storey Staircase for Reaching Entrance: In Rize, an 8-storey building was constructed on the slope, but since there was no access to the ground floor of the building, an 8-storey staircase (without an elevator!) is added to the building. The sentences sound like a rigmarole, but the photo is not montage.
Wooden Bridge Since 29 Years: In Artvin, a retired man built himself a bridge on the river, in order to easily reach his house from the main road. When first built, it was a pedestrian bridge only. Then he renovated his bridge on 1995, and designed it for 3.5 tones car pass as well. He also added a barricade for controlled-pass and hold out electricity cables from his house to illuminate the bridge.
Bridge Between Buildings: The residents of the two buildings in Rize have found an interesting solution to fire danger. They decided that in case of fire in one of the buildings, the residents would pass to the other building from the bridge on the 7th floor. Thus, if a fire breaks out in one of the lower floors, they would run up to the top floors and get out from the next building. Residents are proud of their design, saying "Thanks God, we never used it yet and hopefully we won't…"
Mosque on the Road: In one of the villages in Rize, the villagers could not agree where to build their new mosque. In order to build it close to every house, they constructed on the main road, in the middle of the village. The construction lasted for 8 years and when it was finished, a 5 meters high, 3 meters wide passage came up for the cars.
Set down / Set up Minaret: The unique idea is from Rize again. A minaret was built by adding 7 metal barrels to each other. In winter it is set down to be protected from snow-slide, and in summer it is erected again.
Another minaret was designed by a touristic facility owner. He built the minaret using a utility pole near the wooden mosque, and a couple of car tires.
All Keys Open This Lock: An inventor from Black Sea Region invented a lock that can be opened by all the keys. He said: "The thieves can open all the locks anyway. But if you lose your keys, you can't get in your own house. This lock seems so strong that nobody would try to open it, but only the flat owner will know that any key can unlock it."
Elevator to Mosque: An elevator was added to a 3-storey building's facade, to help the old people climb up to the mosque on the top floor.
Not a Labyrinth, but a Crosswalk: The longest crosswalk of Turkey was built in Rize. It is 500 meter long with its all connections, and the pedestrians say it eases their life. The only thing they need is the traffic signs, not to take the wrong exit.
Master Thesis on Rize Architecture: Architecture in Rize was also became a subject to a master thesis in Italy. Özden Kaya, a master student of Politecnico Di Milano wrote her master thesis on "Unusual Paysage Designs". The important part of her study focused on 80 years old Bilal Atasoy's designs. Atasoy hang his house on the slope of a rock by steel cables. Atasoy built his one-room house on the rock 8 years ago. But when he needed more space, he added 3 more rooms to his design. The house is now a 15 meter cantilever on the rock.
Speaking of cantilevers, Atasoy has probably never seen any image of the "Balancing Barn" by MVRDV, or did he?
And finally, I wouldn't dare to say a word about Le Corbusier's Villa Savoye, but I just keep looking the two pictures below:
"Serender" will be another article's subject.
#architecture #Turkey #blacksea #karadeniz #Rize Architecture


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Engineering Division
103 SW 4th Street
Lawton, OK 73501
About Us
The Engineering Division is responsible for the construction administration of capital improvement program projects, city capital outlay projects, and other special funding projects. These duties include the construction administration, survey, design and inspection of projects. The division reviews and coordinates the preparation of plans and specifications by consultants for various projects. Projects include all types of municipal infrastructure such as streets, drainage, water, sewer and other special projects. Other functions include GIS System updates, archiving of city construction plans, responsibility for city construction standards, surveying functions for the city and special studies/reports.
Where Are We Located?
We are located on 3rd St, just east of the Public Works Yard.
Coming off of I-44, go west on SW Lee Blvd to SW 2nd Street. Head south on 2nd St. The road will begin to curve back to the west. Take the first left on to SW 3rd Street. Head south for approx ¾ of a mile.
Get Direction Map
We are located on the east side of the road in a tan with blue trim building.
Engineering Building
News & Updates 06/12/2015
City Standards Updated
CIP Updated


text:
|Sunday, 25. June 2017
|You are not logged in... [Log In]
|Municipal solid waste in the furnace of a moving grate incinerator
Thermal treatment is a solution for treating nonrecyclable and nonreusable waste in an environmental and economical friendly way. Thermal treatment reduces the volume and mass of the waste and inerts the hazardous components, while at the same time generating thermal and/or electrical energy and minimizing pollutant emissions to air and water.
Waste Incineration, pyrolysis and gasification are the possible thermal treatment processes. In modern European waste management waste incineration plays the absolute dominant role. The processes result in residual products from the waste as well as products resulting from flue gas cleaning additives, which afterwards have to be deposited at a controlled site such as a landfill or a mine. After thermal treatment ferrous and non-ferrous metals can be recovered and recycled. Also the grate ash or slag can be recovered for building purposes. Nutrients and organic matter are destroyed and cannot be recovered after thermal treatment.
In the European Union, Directive 2000/76/EC on the Incineration of Waste regulates waste incineration facilities and sets the limits for emissions into the atmosphere and discharge limits into the water. The objective of the Directive is to prevent or reduce, as much as possible, the pollution caused by incineration or co-incineration of waste to the air, water and soil that may affect human health.
Current Practices and Future Management Options of Medical Waste Management in Turkey
© Lehrstuhl für Abfallverwertungstechnik und Abfallwirtschaft der Montanuniversität Leoben (11/2016)
Medical wastes constitute a larger portion of infectious wastes that are potentially dangerous since they contain pathogenic agents. While sustainable management of municipal solid waste, wastewater sludge and construction and demolition waste gain significant attention, medical waste has not attracted the same level of attention as other types of wastes, especially in developing countries. Therefore, medical waste management is still a critical issue in many countries due to ist potential environmental hazards and public health risks.
Solid Recovered Fuel – Optimization of Plants in the Polish Economic Reality
© Lehrstuhl für Abfallverwertungstechnik und Abfallwirtschaft der Montanuniversität Leoben (11/2016)
Waste management and district heating systems, especially in scope of smaller facilities, require a thorough modernization. In addition, the existing model of these systems, especially for small and medium-territorial units, is not able to meet new requirements of the EU policy in the field of resources and energy efficiency. It is therefore necessary to seek for the new model which quality will be closer to the requirements of the overall strategy of the European Commission, whose key elements includes the impact of the project on the social prosperity and the preservation of the principles of sustainable development.
Modelling of Solid Recovered Fuel (SRF) Properties Based on Material Composition – Chloride Quality
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
Producing solid recovered fuels (SRF) is a well-established route for recovering energy resources from municipal solid waste (household and/or commercial). Chloride content critically impacts the quality of SRF. It directly influences operation of thermal processes, having deleterious effects through the high temperature corrosion of the boilers and through demands placed on the flue gas treatment (FGT) system, which could impact emissions control. Whereas design and specification of process plant can mitigate the technical issues associated with the presence of chloride experienced during thermal treatment, processing such fuels is associated with increased capital, operating and maintenance costs. This, at best, restricts the uptake/use of SRF or increases the cost of its treatment towards achieving a reduced chloride content.
Resource Recovery from Waste Using the Input Flexibility of Waste Gasification Technology
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
Nowadays, gasification of waste or biomass is becoming the great interest all over the world. Especially, gasification of municipal solid waste (MSW) has been well-researched in Japan. The development of MSW gasification technology was started in the 1970s in Japan because of oil crisis. Several technologies have been researched and developed. The Direct Melting System (DMS), which is the gasification and melting technology developed by Nippon Steel & Sumikin Engineering Co., Ltd., is one of the developed waste gasification technologies in this era. This technology was introduced for commercial use in Kamaishi City, Japan in 1979. As well as this waste technology, other gasification technologies have been developed for commercial use and installed.
CO2 Capture and Re-Use at a Waste Incinerator
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
Recently a new innovative process developed by Procede Gas Treating B.V. has been commissioned at line 3 of the Twence plant, a Waste-To-Energy (WTE) plant located in the eastern part of the Netherlands. In this process the CO2, that usually is emitted to atmosphere, is in this new application, scrubbed from the flue gas and the obtained pure CO2 stream is used to produce a sodium bicarbonate slurry (SBC). Instead of the conventional SBC flue gas scrubbing process, where dry SBC particles are used, this SBC slurry will be injected to remove the acid components from the flue gas, before the gas is emitted to atmosphere. Due to the implementation of this process the carbon footprint of the Twence installation is reduced. The new SBC plant produces 8,000 tons of sodium bicarbonate annually and to produce this amount of SBC 2,000 ton per year CO2 is captured from the flue gas. The CO2 originates for about 50 percent from biomass.
Manufacturing of Solid Recovered Fuels (SRF) for Energy Recovery Processes
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
This contribution describes manufacturing processes and quality of three types of
Solid Recovered Fuels – i.e. SRF low quality, SRF medium quality and SRF premium
quality – that are used in energy recovery plants. In total, two case studies are reported.
First case study is about the external processing and confectioning of non-hazardous
household, industrial and commercial mixed wastes as well as the internal treatment
and homogenisation of various waste fractions at the incineration plant for production
of SRF low quality that is utilized in a Waste to Energy (WtE) stationary Fluidized Bed
Incinerator. In the second case study, production of SRF medium quality and SRF premium
quality that are used for substitution of primary fuels like coal and petrol coke
in the cement kiln is described. Finally, data on SRF quality of all three investigated
waste types will be summarized and discussed.
Measures to Implement an Advanced Waste Management System in the Czech Republic
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
The Czech Republic is now preparing the new complete revision of waste law.
The transformation of the waste management into the circular economy started through the legislative process in June 2016. Waste management plan of the Czech Republic for 2015 to 2024 clearly specifies waste strategy and priorities for the country. Thus, in the Act on waste the ban on landfilling of recyclable and recoverable waste in 2024, obligatory separate collection of main municipal waste streams including biowaste since 2015 and currently proposed increase of waste landfilling tax with strict recycling targets already in 2018 are only the first milestones leading to smarter waste future in the Czech Republic.
Brave New World – Selected Jurisdictional Pitfalls when Acting on International Waste-to-Energy Projects
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
Over the last few years, Waste-to-Energy (WtE) projects became increasingly international. In times of low interest rates, solid infrastructure projects with their fix return rates are more and more attractive to project developers, international investors as well as EPC and O&M contractors. They attract financial and strategic investors which would otherwise not turn towards these rather long-term investments. Therefore, a continuously increasing number of international players from different jurisdictions is entering the global playing field.
Overview of the Pyrolysis and Gasification Processes for Thermal Disposal of Waste
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
Thermal treatment of waste started in the 1870s in England with the first waste incineration plants and this technology was in short time adopted by many industrialised countries. Starting in the late 1970s waste incineration was blamed for emission of toxic compounds, in particular of dioxins, and public pressure initiated the decree of more and more stringent air emission standards in all countries which, again, induced significant improvement of the environmental performance of waste incineration.
Initial Operating Experience with the New Polish Waste-to-Energy Plants
© TK Verlag - Fachverlag für Kreislaufwirtschaft (9/2016)
Waste-to-Energy plants are an integral part of modern municipal Waste Management Systems. Today recycling and energy recovery from waste are the only methods of dealing with municipal waste. This is demonstrated by Waste Management Systems in countries such as Germany, Sweden, the Netherlands, Belgium, Denmark and Austria, where the municipal waste management is limited solely to recycling and energy recovery from waste. The currently discussed concept of the latest circular economy package can hardly change anything in this matter. Poland, as one of the leaders among the new EU member states (since 2004), has still a lot to do within the scope of recycling and waste-to-energy.
Countries agree on decisive and urgent actions to restore marine world to health as Ocean Conference concludes (09.06.2017)
Trump's decision will not stop climate action (01.06.2017)
The world's first mall for recycled goods (09.05.2017)
Everbright International Nanjing Waste-to-energy Project Phase II Completes Construction and Commences Operation (29.03.2017)
Gatwick and DHL open new waste management plant – becoming the first airport in the world to turn Category 1 airline waste into energy onsite (23.03.2017)
Renergia, a brand new Waste-to-Energy (WtE) facility opened in Canton Lucerne, shows that Waste-to-Energy can provide reliable heat for industries.Category: Incineration / Waste-to-Energy plant
In Slovenia arises one of the largest and most modern waste treatment plants in Europe.Category: Recycling / MBT
The final unit of the incineration plant is one of the most important parts as it has the objective of cleaning the air pollutants produced.Category: Incineration
The biogas produced from the waste can be converted in a CHP to electrical and thermal energy or fed as processed bio-methane into the natural gas grid or used as fuel (CNG).Category: Recycling / Fermentation
The Bio-Dry™ system is a static, aerated and flexibly enclosed reactor for the biological drying of various solid waste matters containing some biodegradable contents.Category: Recycling / MBT
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Health & Wellness Services Division of Student Affairs
Overview Of Staff
Our board certified physicians and experienced healthcare providers care for more than 10,000
students annually. All of our health care providers hold active licenses in the state of WA
and participate in an annual peer review and re-credentialing process.
Meet our team of medical, pharmacy, administrative and health promotion/programming professionals.


text:
Welcome to E-Books Directory
This is a freely downloadable e-book.
Advances in Robot Manipulators
Read this book online or download it here for free
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Advances in Robot Manipulators
by Ernest Hall
Publisher: InTech 2010
Number of pages: 688
The purpose of this volume in Advances in Robot Manipulators is to encourage and inspire the continual invention of robot manipulators for science and the good of humanity. The concept of eclecticism for the design, development, simulation and implementation of a real time controller for an intelligent, vision guided robots is now being explored.
More Sites Like This
|Science Books Online
|Books Fairy
|Maths e-Books
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The operation
The ICAC's inquiry into Liberal Party slush funds adjourned on Tuesday after more than three weeks of public hearings. It will resume on August 4 and is expected to be completed within the month.
The allegations
The commission is investigating allegations that former state energy minister Chris Hartcher and other Liberal Party figures used front organisations to "wash" illegal donations to the party before the last state election.
Property developers, who have been banned from making donations since 2009, are allegedly among the donors.
The commission is also examining whether Hartcher and others did favours in return for the payments.
The key players
Chris Hartcher: Former Liberal energy minister and alleged "mastermind" of corrupt scheme to channel illegal donations from developers and other sources into a "sham company" called Eightbyfive which issued fake invoices for work. Also accused of "laundering" $4000 in Liberal Party cheques.
Tim Koelma: Former adviser to Hartcher who set up Eightbyfive. Claims the company was paid for political and media advice. The inquiry has heard there is not one "crummy, flimsy piece of paper" to support his claims. He says a flood in his garage destroyed documents that could prove his innocence.
Ray Carter: Long-term staffer to Hartcher. Admitted he knew he was collecting money from banned political donors. He claimed Hartcher also knew of the practice. Carter also claimed that he handed to Hartcher the $4000 Hartcher had laundered through his old law firm and then into an account controlled by Carter's partner.
Chris Spence: Member for Central Coast seat of the Entrance. Allegedly received more than $100,000 from Eightbyfive which supported him during election campaign. Claims he did work for the company but his computer hard drive failed and he cannot produce documents.
Darren Webber: Member for the Central Coast seat of Wyong. Received about $50,000 from Eightbyfive which supported him during election campaign. Claims he did work for company but does not have any records because he replaces his phone every year and his computer also died.
Nathan Tinkler: Larger than life mining entrepreneur who has denied trying to bribe former Labor MP Jodi McKay. When McKay refused to accept donations, the Tinkler group, who wanted a billion-dollar coal loader, gave money to fund a campaign against her. When the commission started investigating Eightbyfive, which allegedly received $66,000 from a Tinkler company, Tinkler emailed a colleague on April 19, 2013: "Who is ICAC?" The reply prompted him to respond: "Oh mate u r f--king kidding me..."
The Gazal brothers: The property developer brothers behind the controversial Orange Grove clothing outlet at Liverpool, which has been given the green light under the current state government. Allegedly paid $137,000 to Eightbyfive in return for favours.
Nick Di Girolamo: The former chief executive of Obeid-linked company Australian Water Holdings. Gave the $3000 bottle of Grange that ended Barry O'Farrell's premiership, and allegedly arranged for AWH to pay $183,000 to Eightbyfive in return for favours.
Marie Ficarra: Upper House Liberal MP who denied she asked her friend, developer Tony Merhi, donate $5000 to Koelma's company Eightbyfive. Repeatedly referred to her sick dog. "It's not easy to take blood from a miniature schnauzer," she said. Eventually Watson snapped: "Please don't mention the dog again."
Aaron Henry: Young Liberal and former staffer to Chris Hartcher. Sent an email to Terrigal Young Liberals outlining "The Rules of Black Ops", modelled on the rules from the cult film Fight Club. Henry said "Black Ops" referred to the practice of going out at night during election campaigns and removing opposition posters and placards. The rules included "Black shirts and black shoes" and "When someone yells stop, goes limp, or taps out, the night is over".
Charles Perrottet: The younger brother of Finance minister Dominic Perrottet. A former staffer to Chris Hartcher denied providing the bank details of Eightbyfive so that a banned developer, Tony Merhi, could donate funds. May have to give further evidence in August.
The next phase of the inquiry
ICAC investigators need an adjournment to examine new evidence implicating former police minister Mike Gallacher in "serious electoral funding irregularities". Gallacher will give evidence when the inquiry resumes on August 4, along with a number of other Liberal Party witnesses. The former president of the NSW branch of the party, Liberal Senator Arthur Sinodinos, and its former chief fundraiser, Paul Nicolaou, will be asked about their knowledge of an alleged Liberal Party "front" called the Free Enterprise Foundation. The foundation was allegedly used to "wash" illegal donations from property developers and send them back to the NSW branch.
The report
The ICAC is expected to release a report on Operation Spicer before the end of the year.
What happens after the report?
If the ICAC makes corrupt findings against any of the key players, it may also recommend the Director of Public Prosecutions consider laying criminal charges.
It is up to the DPP to consider whether or not to proceed.
''Operation Spicer is mainly concerned with political fundraising in the Liberal Party, and the way in which unscrupulous businessmen sought to buy political influence.''
- ICAC Counsel Geoffrey Watson SC


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The Washington Post ran a story yesterday that spoke of not only the allegations against Gothard and his removal as head of IBLP but also giving mention to other recent scandals involving sexual abuse in the Evangelical world. They interviewed some of the ladies Gothard preyed upon who've told their stories through Recovering Grace and also pointed out that the Duggars follow his teachings and that Bill Gothard has been embraced by many in the Republican Religious Right. … [Read more...]
Bill Gothard Scandal Finally Picked Up By Mainstream Media
March 1, 2014 by Suzanne Calulu
Filed Under: Announcements, Bill Gothard Sexual Harrassment, blindly following leader, Child Molestation, Christian Control of Government, Confession, Control, Dangerous People, Devaluing Children, Doug Phillips Adultery, Duggar Media Obsession, Duggars Raising Children on Television, Grace fired from Bob Jones University, Group Think, Hot Topics, Isolation, Markers of a Cult, Mega Churches, Must Read! ~ NLQ Readers Choice ..., NLQ Miscellany, NLQ News Links Saturday, NLQ Open Comments, Patrick Henry College Sexual Abuse, Pensacola Christian College Sexual Abuse, Purity Culture, Quiverfull in politics, Quiverfull in the News, Rape Culture, Rapists Protected by the Church, Sexual Abuse at Bob Jones University, Sexual Abuse in the Church, Spiritual Abuse, Vision Forum Inc Closing, Vision Forum Ministries Closes, Worshiping The Law (Bible) Tagged With: Advance Training Institute (ATI) / Institution for Basic Life Principles (IBLP) (Bill Gothard), bill gothard, Bob Jones Uni, Jim Bob & Michelle Duggar, Sexual Abuse, The Duggar Family


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Mineral oil: don't knock it 'til you try it!!
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Join Date: Aug 2011
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Thursday, February 14, 2013 at 03:53AM
Me, too! And I hope I can hide it from my husband.
Lol! I don't even bother. He hasn't made any comments, but I know he notices all the products and such. (Tho I don't think I'm as bad as some, I must say... At least I hope...maybe? Lol!)
I fully realize he thinks I'm half crazy. Lol! But he's a guy, and has short, straight hair. HE COULD NEVER UNDERSTAND!!! Lol!!
2c/3a/3b, Fine/med, Dense,
high porous, & normal
Lo-poo - Renpure Keratin, SMR, Giovanni SaS
clarifying - mix Johnson's H2T with low poo
Co-wash - Suave Nats. clar. or coco.
RO - GVPCB, Ion Eff. Care, TN.
LI - SMR, Cure Care, or RO
Seal - MO or Jojoba
DT - + honey, ACV, & EVOO to SM masque & GVPCB
PT - Gelatin!, CNPF
Styling - FSG, AIF, BRHG, LALS/PS, GF curl spray gel, SM Souffle/Smoothie, LOOB
VO5 Hairdressing


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China raised fuel prices for the second time this year, hiking gasoline and diesel by 6.5% to 7% respectively. Prices went up by 600 yuan ($94.90) a metric ton, the biggest hike since June 2009. The new prices are effective on Tuesday. Many newspapers in China put up headlines like "The age of 8-yuan gasoline has come". And not surprisingly, on Monday evening, long lines before gas stations were spotted all over China.
Hangzhou, Zhejiang Province
Jiujiang, Jiangxi Province
Fuzhou, Fujian Province
Anshan, Liaoning Province
Weifang, Shandong Province
Zhengzhou, Henan Province
Nantong, Jiangsu Province
Pictures from Sina Weibo
[...]Long lines in front of gas stations the night before gas price rise in China | Offbeat China[...]…


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I agree completely with your editorial endorsement of Barack Obama for our next president (February 2008).Indeed, the more I hear his speeches and about his
India is the fastest growing source of illegal immigrants, according to the U.S. Department of Homeland Security.The agency estimates that there are 270,000 undocumented Indians
Barack Obama trounced Hillary Clinton in the Democrats Abroad Global Presidential primaries in India by 71% to 28%. Obama received 244 votes from American Democrats
A New York Court has ruled that an Indian doctor missing since Sept. 10, 2001, died while trying to save lives at the Twin Towers.
InfoUSA has pulled a Super Bowl ad criticized as being racist.In the ad, animated Panda bears with Chinese accents save their business, Ling Ling's Bamboo
The Wisconsin Alumni Research Foundation is suing Intel for infringing on its patent in the development of its Intel Duo Core chip. A lawsuit filed
Mahindra & Mahindra Ltd. plans to assemble and sell light pickup trucks in the United States by 2010.Pawan Goenka, president of the company's auto services,
The survey measures compensations in five job categories
India's software service and outsourcing industries are expected to grow 33 percent in 2008
How Facebook Gave Birth To An Industry.
In recent weeks, several large equity offerings, including those from reputable business houses, have struggled to hit their targets. India's stock markets have been volatile,
By 2025, possibly even earlier, foreign born Americans will account for 15 percent of the population.
Progressive Desi DJ Collective Celebrates Ten Years of Groundbreaking Music
A Brooklyn jazz band breathes new life into Nusrat Fateh Ali Khan's sufi music.
Nothing. But Indians sure know a lot!
Bobby and Kal are no Barack.
Liberate our seniors - to work!


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Hi all, here the situation:
I use BT3 on my HP Laptop (dv6701ca). I boot from the USB but the weird things is that I have to plug the power adapter to be able to boot, if I unplug it or remove it during to boot to only use the battery, everything freeze and I have to plug back the adapter for the booting process to continue!?!
Have you ever seen that or do you have any idea what can cause that?
thx all,


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Please note that embroidery orders not qualify for the free shipping offer.
Read Customer Reviews
A great all-around boot that's ready for any challenge. It features a dual-density, rubber Goodyear welt-construction outsole for gripping power, and a lightweight and comfortable high-abrasion blown rubber, slip-resistant insert. A waterproof membrane will keep you dry. When things get a bit sloppy, you'll appreciate its oil- and chemical-slip resistant properties. Tan brown, oil-tanned leather.


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Jorge Castro, 45, speaks about farewell fight with Roberto Duran, 62
A press conference was held in Argentina to announce the clash of legends between 45-year-old former world champion Jorge "Locomotora" Castro (130-11-3, 90 KOS) and the incomparable 62-year-old Roberto "Mano de Piedra" Duran, in what will be the rubber match of their trilogy on September 7 at the Luna Park Stadium in Buenos Aires, Argentina, in an event presented by Mario Margossian/Argentina Boxing Promotions and Full Box. Duran was not present, but Castro spoke about the fight.
"I am very happy to be here and that this is the farewell that so many wanted. Every boxer wants to leave the sport in a place where they have fought all the big names, like at Luna Park, and we are doing that. And if that were not enough, with someone big like 'Hands of Stone" Duran!
"I never walked away completely. I'm always stayed in shape physically, even sparring with some kids, but obviously less than before. It's going to be a party. I hope that people will be happy with what I did in my career and I really want to enjoy the entire event. I have no doubt that will be a unique night."
Their first bout was in February 1997 in Mar del Plata, Argentina and Castro won a controversial ten round decision. The second fight, in June 1997, was won by Duran via decision over 10 rounds in the Nuevo Panama Gym, which today is named the Roberto Duran Arena. The match will be a three-rounder.
The event will also include former world title challenger Javier "The Beast" Maciel (26-2, 19 KOs) against Alejandro "El Flaco" Falliga (23-9-4, 10 KOs) and IBF female super flyweight world champion Deborah "The Gurisa" Dionicius (13-0, 4 KOs) against Olga "La Monita" Julio (15-14-1, 10 KOs).


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Noticeably worse than previous month(s)... I guess it's a bad combo of holidays, summer and slowness of envato sites due to ddos attack.
Yup, a lot of PhotoDune (and since recently, even VideoHive) reviewers are from Hungary, as it seems
Thanks for the badge I also got it
However, it is so awesome that when I press the "That's awesome!" button, it does nothing and popup notification about badge stays there until I cancel it by clicking on the "X" button
(it happened before as well, a few weeks ago when I got some other badge)
Using the latest version of Chrome.
Creattive said
I guess more than this official statement is not available: http://videohive.net/forums/thread/state-of-the-stock-footage-queue/179558
Yeah, I know about that statement, but it's been a month since that statement has been given.
I guess it would be at least polite to address this once again, even if just to give another generic "we're working on it" statement, don't you think?
It's been many weeks now since the review time is longer than 2 weeks.
Can we get an official statement from somebody from the staff, at least about what is being done to rectify this chaos? I believe we deserve to know if and when the situation will be back to normal, because it influences our workflow(s).
Thanks in advance.
Yup, same. It seems like it has to do something with site outages (see here: http://videohive.net/forums/thread/intermittent-site-outages/183714 )
Be patient, hopefully it will be settled
(P.S. I missed last month's payout because of my mistake, and now this happens… )
- first reviewer missed something hard-rejection worthy and then the second one saw it and rejected your item,
- second reviewer made a mistake.
Whatever the reason, your best option is to post directly to support. They will forward your ticket to the reviewing team and then they will see whether there was a mistake or if it was justified hard-rejection.
Hope this helps.
Any chance we can get somebody from staff to give us an update of the situation?
SpaceStockFootage said
Can we have a T-Shirt if/when we get to $70K? I'll trade in the Tuts+ membership if that helps.
Can we? Can we, please?
I always add a small .txt file as a way to thank the buyer and to provide links for my other portfolios within Envato ecosystem (videohive, audiojungle, photodune, etc…).


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Tag: westminster house
Pondering Ideas of Reuse
I was thinking this morning that when they do demolish the old house, could I reuse any of the old materials, and what would the new owner allow to be taken. I plan to go down this weekend with my cousin Stephanie to take pictures before it is demolished, but I may also look around and see if there's anything we can ask to take.
Honestly I wouldn't mind having the windows, if nothing else we could use them in new animal pens. Doors could be useful, maybe. I know I really liked the old hardware on them, and having those could just be my "remembrance" item from the house. Maybe even some of the wood floor could be salvaged. I know dad wants the old gas stove, even though it's older than me. I'd definitely like to have the electrical box and the hot water heater, since we paid for and installed those ourselves.
None of the cabinetry is any good, and the oil based heater is shot. I can't think of much else at this point, but I wouldn't mind doing a little diving.
No Savings Contribution and Demolition
I decided, with much hesitation, to stop all savings contributions for now. It wasn't necessarily hurting me to keep adding to my savings, but it was slowing down the debt repayment process. So now I will be able to tackle this with hopefully more force just to get it out of our lives for good.
My credit card also came for my father today for my account. I may hold off giving it to him until his balance is paid off. But I am still undecided about that.
This week I find out if his tractor supply account attempts to go to 30% APR, in which case I'll pay it off now instead of later.
And I also have to call the local tax people to see if they've received a payment from my escrow account. By the looks of my Chase account, it doesn't look like they have, so I'm going to bring a copy of my tax form to work tomorrow to copy and send again so I can get the discount.
Dad said the old house is scheduled to be demolished as soon as the new well and septic are put in. I'd like to ride down and get pictures before it is, considering I was under the impression it wouldn't be demolished (surprisingly). There's no way he could have renovated that thing anyway.
The House Saga has Ended
For the most part.
Our old house we were living in has finally sold. I mentioned before that my circle of amusement would be complete if the house sold for $100k, which is what I offered back in April 2008. Well it did.
For those unaware, the short backstory is that our 90+ year old landlord had died leaving 3 farms and no Will, with no children. His entire estate was to be divided up between 6 nieces and nephews. He owned 3 farms, one of which was the one we've been renting forever. The other two sold for 3 million a piece, but they wanted double the price ($200k) of what ours was worth ($100k) because ours was condemned. So they gave us 30 days to get out. We took all the livestock to the auction, plowed up all our crops, and left a lot of trash behind.
We moved in with my Aunt and lived with her for 2 months while we searched for a place to live. We eventually bought our awesome house here in Gettysburg and are all the better for it.
Anyway, back to the old condemned house. They had it listed for $124.9k with no bids. An old neighbors son actually offered $100k cash and the estate took it. The guy apparently plans to fix it up rather than mow it down and rebuild, which surprises me.
He did say, however, that if there's anything we want off the property after settlement that we can come take it. There are a couple things I wouldn't mind having, so that was nice of him.
He also asked us if we wanted to rent it once it's rebuilt. Dad said "Hell no, we have a good house here in Gettysburg." so that was that.
In a way I'm sad to see the house sold. I'm glad it sold for what it did because it serves the greedy bastards right. I'm just sad to think of it changing and it permanently not being ours anymore. It was literally the house I was born in and one of many houses I was raised in.
Maybe he'll let me take some more pictures before they start doing whatever they do to it. It will be a good investment for him, and the neighbors are happy that he's the one buying it.
Handyman Special, Detached Garage!
Dad was down in the old neighborhood again since he still does tasks for some of the old ladies that live there. They had realtor brochures up for grabs, so dad decided to grab one.
"Handyman Special, Detached Garage, Beautiful Lot!"
I can give you a guess of which one of those is true, and it ain't the first two.
Our neighbor (who is also my dads cousin) said a few people have stopped by to look at it. Most don't get out of their car. One was an excavator and said it would cost him at last $40,000 just to tear down everything and haul it away. THEN he could start spending the money to build a house on it. The $180,000 is not worth that. He talked to one of our other neighbors on the other side and they told him that we had offered $150,000 to buy it and the guy responded "And the dumbasses didn't take it?".
This is my amused face. Is it wrong that I hope it sits for a couple years?
In addition, the detached garage is not a garage. You might be able to fit a miata in it, but it was built narrow for a buggy, not for automobiles. It has a dirt floor, spaced wooden walls (meaning you can see through them) and a leaky rusty tin roof.
They also have not cleared out -any- of the trash that was left there. It looks a shambles.
The realtor asked our neighbor cousin where the well and septic were. She told them it doesn't have a septic and the well is buried underneath the sidewalk. She's sorta right. The septic is supposed to be under the hill with the walnut tree on it, but it hasn't been checked in over 29 years. I imagine it's a steel tank, probably leaking heavily, and I'm sure the tree's roots have pierced through it by now and are the only thing keeping it from collapsing. I wouldn't want to be the one to dig that up.
They are also advertising it as two bedroom. It's not two bedroom, it's one bedroom. My father just happened to sleep in what was supposed to be a 6×9 closet all these years.


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New Translations of Experimental Yiddish Poet Celia Dropkin Published at Jacket2
Now this is news: A decade of translating work from Samuel Solomon, Jennifer Kronovet, and Faith Jones has led to the publication of new translations of experimental Yiddish poet Celia Dropkin (1887-1956) at Jacket2, with an amazing note from Jerome Rothenberg on the poet:
Among the more experimental Yiddish poets in early twentieth-century New York, Dropkin (1887-1956) was significant both for her exploration of open verse as a compositional strategy & for her assertions of female desire beyond the limits observed by most of her contemporaries, both in Yiddish & in English. Born Zipporah Levine in present-day Belarus, she wrote first in Russian but turned to Yiddish on arrival in New York circa 1910, where she participated in the already active Yiddish poetry world, including the experimental In-Zikh (Introspectivist) poets, while developing more markedly transgressive themes than theirs: sexuality, depression, guilt & longing, fury, violence, even at its limits the representation of sado-masochism & other taboo, once hidden subjects. Her work in that sense is a further confirmation of Kenneth Rexroth's observation of a Yiddish avant-garde & Futurist presence in his own early years in New York: "A good case could be made for the claim that the best writing done in America in the first quarter of the [twentieth] century was in Yiddish. I don't think it's really true, but it is sufficiently true to be passionately arguable in one of those passionate arguments that used to sprinkle the whiskers with sour cream in the Café Royale." And despite Kenneth's charmingly flippant tone, the active historical presence of two languages & their attendant poetries in a single American city is itself worth noting.
Read the poems here. Astounding work. Dropkin was also a painter; that's hers, above. And keep your eyelids way up for the forthcoming bilingual book from Tebot Bach.
Tags: Celia Dropkin, Faith Jones, Jerome Rothenberg, Samuel Solomon
Posted in Poetry News on Tuesday, May 14th, 2013 by Harriet Staff.


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I am trying to trade my 96 jetta 2.0 plus cash for a reliable vr6
I have a 1996 jetta with a clean title, it has 183k miles on a stock 2.0 motor and a 5spd transmission. I know it is high miles but the motor runs strong. Has beige interior with Manuel windows, power locks and a sunroof
The Good
6 and 1/2 inch Boston speakers all the way around
After market ken wood head unit
Brand new front tires
75% tread on the rears
Recently replaced stock suspension
Recently replaced alternator
New vacuum lines
Timing belt redone about 6k miles ago
Serpentine and v belt done about 6k miles ago
E-brake lines replaced about 6k miles ago
Both control arm bushings replaced 6k miles ago
Both tie rods replaced 6k miles ago
Both ball joints replaced 6k miles ago
Heater core replaced 3k miles ago
The Bad
Car was in a front end collision and I only had black parts so from the windshield up is black.
Front seats are worn but no holes
Little spider crack in windshield, didn't spread all winter and is out of drivers view so should pass
No AC, need new condenser
Runninng on 14" steelies
Hand crank windows
Clear coat peeling all over ( if you bought I would suggest painting )
If you want pictures I can send from my cell phone and for fastest response please text or call 301-697-5331