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Meet new people!
Learn a sophisticated, sultry dance!
Featuring hot swing, blues, jazz, pop and R&B music!
What is the Madison West Coast Swing Club?
The Madison West Coast Swing Club is a social, non-profit organization dedicated to enjoying and promoting West Coast Swing (or WCS) dancing. We started in 1996 with twelve enthusiastic WCS dancers. Since then, our membership has swelled to more than ten times that and is still growing!
What is West Coast Swing?
West Coast Swing is a laid-back, improvisational, smooth and sultry dance done to a wide variety of slow-tempo music, including R&B, pop, blues, swing, jazz, and country. You'll recognize artists such as Christina Aguilera, Michael Jackson, Roomful of Blues, and Ray Charles. Our emphasis is on having fun and playing with the music, rather than wild, gyrating acrobatics.
When does the club dance?
We host weekly and monthly dances. Beginner and Intermediate lessons are offered Wednesdays, followed by a dance. Our club also hosts visiting professionals from the West Coast Swing community at the club's monthly dances.
No partner necessary. Singles and non-members are welcome!
Will I enjoy dancing?
It's easy to find out if you like to dance and to dance West Coast Swing in particular. Simply come to one of our dances or sign up for lessons. This dance is done to a variety of music--from blues to pop to funk to country, so you can use it almost anywhere you go. Our current members enjoy learning more intricacies of this playful, sophisticated dance as they progress, and newcomers enjoy meeting a group of friendly new people. You can join our club at any of our events. As a member you will receive emails about local and regional West Coast Swing dancing, plus discounts on our events.
In the Beginning...the Origin of the Madison West Coast Swing Club
By Bill Mitchell
Gary Oien spoke the words first, "We gotta start our own club."
We all agreed. Gary, Sue, Carla and I were returning to Madison, Wisconsin from a West Coast Swing Workshop and Dance at the Minneapolis Rebels Swing Club given by a visiting professional named Jeannie Tucker. The Rebels' members were very hospitable and even insisted that the four of us do a spotlight dance (gulp!) in front of a hundred strangers and Jeannie. Their president, Judy, seemed thoroughly delighted that we would drive 4-1/2 hours just to participate at a Rebels event. What none of them knew, and what we knew all too well, was the scarcity of West Coast Swing dancing opportunities in Madison in the fall of 1996. It was frustrating for the dozen of us who had been dancing West Coast for at least a year or two.
But wait, why would we try doing something called West Coast Swing in the first place? Isn't it the state dance of California? Well, forget about West vs. Midwest. It's just a great dance! I learned it about six years ago when I lived in Tucson. When I moved to Madison, few people knew what West Coast Swing was. Occasionally one of the local dance instructors would teach a class in West Coast, but it was simply not the wonderfully sexy, funky, and improvisational dance I had seen in Arizona.
In August 1995 at a Chicago dance, we met some other nice folks from Madison, including Sue Pederson and Carla Starks. Eventually I met and danced with a Chicago-based West Coast Swing instructor, Janis Kenyon.
The idea of Janis giving a workshop in Madison was proposed and it happened that fall. The workshop was attended by 24 people, about half of whom caught the West Coast bug and continued dancing wherever there was music and floor space available. That workshop also paired up Michael Collins and Carla Starks Collins for the first time!
After that, our unofficial West Coast group took advantage of any opportunities we could to attend workshops and dances. In February 1996 we took a road trip to Minneapolis for a small, but wonderful, Swing Dance Convention. It included workshops by such West Coast Swing notables as Michael and Mari Kiehm and the effervescent Skippy Blair. That September, we were lucky enough to have Ginni Hustad (of Step-n-Out dance apparel) bring Carrie Lucas to Madison's Sapphire Studio for workshops. But after the great workshops and road trips, the frustration was always the same: where do we dance?
Finally, after yet another Jeannie Tucker workshop in Minneapolis and after we had seen what a swing club could offer enthusiastic dancers, Gary just stated the obvious. We gotta start our own club. Upon returning to Madison, Gary began the quest to locate a venue for our music and weekly dance instructions. After he found the friendly and supportive manager and DJ (Buzz) at Flashbacks, we knew we had a home. Initially, we told them we expected about 15-20 people each week--then crossed our fingers.
Since then we've grown tremendously and West Coast's popularity is flourishing. Local interest has grown to the point that we can host professional instructors. Great idea Gary!


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Looking for the best of the best photographers in Fort Worth, TX? You have came to the right place. We are a group of photographers. Each with different styles and personalities to fit your photographic needs. Check out "Photographers" to find the perfect match.
"We go the extra Yards in Stockyards Photography."
Follow us on Facebook: www.facebook.com/stockyardsphotography

subset: commoncrawl


text:
Compare 2006 Sedans by city or highway MPG! Just one of the many things possible with the Rank-By-Specs Compar-A-Graph!
|[an error occurred while processing this directive]
"Raceline" is a racing news program covering the NASCAR Nextel Cup, Busch Series, and Craftsman Truck Series.
New programs air Weekly
More Show Information
July 2, 2007
Want more information? Search the web!
Search The Auto Channel!


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Decision makers
- New York City Council and New York State Assembly
New York City Council and New York State Assembly: Raise the minimum wage to $15 and Quality of Life in NYC
New York City residents are struggling to survive on the minimum wage of $7.25 per hour. A large part of New York City residents are currenty living at or near the poverty level and things NEED to change for our residents so our city can truly flourish.
*15$ A Hour is just the beginning of a struggle to improve the quality of life and economic justice for the 99% in New York City for the following reasons:
1. Your quality of life is adversely affected if you spend more than 50% of your net income or salary on basic necessities like rent, food, electricity, health care and other needs.
2. Debt and interest rates from credit cards and or student loans are higher than monthly salaries.
3. The average rent on an apartment in New York City shot up to $3,017 a month this quarter, according to data compiled by REIS, a real estate research firm. Even at 15$ an hour, a 40 hour work week equals 2,400 a month BEFORE taxes.
3. In 2011, 46 percent of New Yorkers were considered either poor or almost-poor. (The income gap in New York City is comparable to those of some sub-Saharan African countries) according to the NY Times.
4. The New York Times reports the average price to buy a Manhattan apartment was a "stratospheric $1.425 million" this spring.
5. 'Official' City estimates show that One out of every five New Yorkers, 1.7 million people, lives below the federal poverty line and one out of every ten New Yorkers has a full or part-time job and still lives below the poverty line.
6. Multi-Trillion dollar banks like Chase make millions of dollars off of public assistance recipients by processing their cash allotments from their food/cash assistance cards.
7. According to a study by Picture the Homeless, there are more habitable and abandoned buildings in New York City than there are homeless people.
8. The City provides shelter to more than 20,000 homeless children each night costing it more money than the cost of providing rental subsidies to house them in apartments.
9. It would cost the city 33$ a day to house someone in an apt but instead subcontractors charge the city 137 dollars to house in a shelter. And It costs $36,000 a year to shelter a homeless family in New York City. In comparison, a rental voucher is $10,000 a year says Patrick Markee, a policy analyst at the non-profit Coalition for the Homeless.
10. A progressive tax on Wall Street/Stock market would bring in billions of dollars potentially paying for the entire subway system, salaries of teachers, firefighters, public and private sector workers, improve public services, community and health care centers and hospitals, as well as a variety of others needs for the 99%.
- New York City Council and New York State Assembly
Raise the minimum wage to $15 and Quality of Life in NYC
OccuEvolve (OWS) started this petition with a single signature, and now has 49 supporters. Start a petition today to change something you care about.
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text:
Available ebook formats: epub mobi pdf rtf lrf pdb txt html
I am a stay-at-home mom of four school-aged boys and the proud wife of a veteran of the US Air Force. I live in Montana where I am fortunate to spend my days creating new worlds with characters I fall in love with. I have written fifty romances and have plans to write many more in the years to come.
Review by:
Shelley Chastagner
on April 02, 2013 :
Sweet and funny. The characters are likable. The story line holds your attention. Great for a rainy weekend or vacation read. A couple of romantic scenes make this an adult read.
(reviewed 25 days after purchase)
Review by:
on Dec. 07, 2011 :
what a great idea for a book! loved it---loved it---loved it!!!!!!!!!!!!!!!!!!
(reviewed 39 days after purchase)
Review by:
beth beale
on July 11, 2010 :
Terrific Historical romance novel!!!!
(review of free book)
Review by:
Doug Pardee
on June 03, 2010 :
A solid story-line and a group of interesting characters make for good reading.
The writing isn't as artless as in many romance novels, but it still wasn't as polished as one finds in most other fiction genres. If you're just fine with how romance novels tend to be written, you might give this five stars. But I just couldn't overlook lines like this:
"This is getting annoying," he said, obviously exasperated.
This book is quite well-edited. There are still a few incorrect words, notably "hawk" used to mean "hock". But those are notable because the rest of the editing is so clean.
(reviewed 83 days after purchase)


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Kohl's CEO Mansell reportedly planning management shake-up
A report that Kohl's Corp. CEO Kevin Mansell plans to shake up the management at the Menomonee Falls-based retailer "is still just speculation at this point" but makes sense given the department store chain's difficulties in recent years, an analyst says.
"The board is surely starting to run thin on patience," Morningstar Inc. analyst Paul Swinand said Tuesday in an email, after The Wall Street Journal quoted a person familiar with the matter as saying that management changes were coming. "I'm sure this wet/cold winter/spring hasn't helped anyone."
If the changes occur, they would represent a continuation of a rolling shake-up at Kohl's that began in 2012.
The most recent development in the evolving situation was the resignation in March of Donald Brennan, the company's chief merchandising officer and one of its most highly paid executives.
Kohl's disclosed the move in a securities filing in March that said nothing about where Brennan, 53, was going.
Brennan's departure leaves "a big seat open," Swinand said.
Spurred by a subpar showing during the Christmas 2011 selling season and inventory problems the following spring, Mansell began reconfiguring Kohl's merchandising, marketing and information technology leadership nearly two years ago.
That effort to lift the once-stellar retailer's mediocre performance of recent years already has seen several important changes.
The company's chief marketing officer quietly left in the fall of 2012, and in May 2013 Kohl's disclosed the resignation of John Worthington, its chief administrative officer and at the time its third-highest-paid executive.
Meanwhile, the company brought in highly regarded Starbucks executive Michelle Gass to fill a newly created, top-level post: chief customer officer. Gass instantly became one of Kohl's top earners and, with Brennan gone, appears to be No. 2 in authority behind Mansell.
Gass is in charge of all marketing and e-commerce efforts — crucial areas for any retailer.
Kohl's spectacular rise as a midpriced department store chain during the 1990s and early 2000s made fortunes for many who held its soaring stock. But in recent years the firm has seen profits and same-store sales stall.
Over the five years from February 2009 into February 2014, meanwhile, its stock gained 49% while an index of peer companies rose 243%, according to Kohl's most recent annual report to securities regulators.
Mansell has done a good job in a difficult retailing environment, said Geoffry Penn, a managing director and founding principal at North Star Asset Management in Menasha.
He said Kohl's has been up against lousy weather and "less than rational competitors" such as financially troubled J.C. Penney, which has been dangling unsustainable offers to try to lure back shoppers.
Meanwhile, Penn said, Kohl's has handled the financial side of its business well.
"They bought back a ton of stock and they managed their accounts receivable and inventory pretty well, so from a financial perspective they're doing all the right things," Penn said. "It's just a tough environment."
The company will report its first-quarter earnings Thursday morning and holds its annual meeting that afternoon at Discovery World.
Kohl's operates 1,162 stores across 49 states.
Indianapolis developer proposes 274 apartments at former Pabst complex
Peptimed raises $200,000 to aid development of cancer-fighting drugs
Ballpark Commons could get $26 million in city funds, study suggests
'Professional plaintiff' uses credit law to threaten companies, win $230,000 in settlements
PreCheck air travelers aren't always getting what they paid for
Tom Still: Choosing the correct tool to boost start-ups can be a balancing act
Investment Trends: Proliferation of mobile devices should fuel network infrastructure stocks
Whitestone Station bringing stores, restaurants, over 300 apartments
WEDC identifies priorities for economic growth
Steve Jagler: After-hours enrichment sparks Sobic's executive journey to the NFL
Business Wire
Recent national business updates from the Associated Press


text:
Magic Silk - Peek-a-Boo Set Black Queen
From the Magic Silk Exposed collection, this style includes a triangle peek-a-boo bra featuring functional ribbon bows with adjustable shoulder straps, butterfly clip closure in back and matching crotchless short set with a bow accent.
Hand wash, cold. No bleach. Dry flat.
58% Nylon, 40% Polyester, 2% Spandex
Related products by keyword...
You may also like...


text:
Office of Emergency Services (OES)
Mission StatementThe mission of the El Dorado County Office of Emergency Services is to prepare the people in El Dorado County to prepare for, respond to, and recover from emergencies which threaten life, property, or the environment.
Operational AreaIn 1994, Board of Supervisors designated the Sheriff's Office responsibility for managing the County's Office of Emergency Services (OES).Sheriff's employees assigned to the OES work in collaboration with Fire services, Emergency Medical Services, hospitals, schools, and public and private agencies to implement preparedness programs, develop emergency response plans, and conduct training drills.
Emergency Operations Center Activation GoalsActivation of the Emergency Operations Center facilitates the sharing of information and resources between the county, the Operational Area cities, and other agencies. This allows EOC staff too efficiently:
Volunteering and Training opportunities
Quick Links
© County of El Dorado


text:
You are currently viewing the abstract.View Full Text
Viral clearance during hepatitis B virus (HBV) infection has been thought to reflect the destruction of infected hepatocytes by CD8+ T lymphocytes. However, in this study, HBV DNA was shown to largely disappear from the liver and the blood of acutely infected chimpanzees long before the peak of T cell infiltration and most of the liver disease. These results demonstrate that noncytopathic antiviral mechanisms contribute to viral clearance during acute viral hepatitis by purging HBV replicative intermediates from the cytoplasm and covalently closed circular viral DNA from the nucleus of infected cells.
↵* To whom correspondence should be addressed. E-mail: fchisari{at}scripps.edu


text:
Maternity Enquiries +44 (0)20 7390 6200
Children's Enquiries +44 (0)20 7390 8020
Gynaecology Enquiries +44 (0)20 7390 6200
You are here: The Portland Hospital
/Medical Professionals
/Find a Consultant
/Mrs Pradnya Pisal
Consultant Gynaecologist
Menorrhagia, polycystic ovarian disease, prolapse, fibroids, endometriosis, ovarian cysts, laparascopic myomectomy, laparascopic hysterectomy, recurrent pregnacy loss, early pregnancy care, ectopic pregnancy, miscarriage, antenatal screening including the harmony test.
Barnet & Chase Farm NHS Trust
The Portland Hospital
0208 367 8999
0208 082 5667
Mrs. Pisal has worked as a consultant gynaecologist and obstetrician for over 11 years. She currently works in the NHS as Senior Consultant at Chase Farm Hospital. Privately, she sees patients at The Portland Hospital for Women and Children, Central London and the BMI King's Oak and the BMI Cavell Hospitals in Enfield, North London. Her areas of expertise include Recurrent Miscarriage, Ultrasound Scanning, Fetal Medicine and Advanced Laparoscopic Surgery including Robotic Surgery.Mrs. Pisal is the Royal College Tutor at Chase Farm Hospital responsible for teaching and training junior doctors. She is also the Regional Ultrasound Training Co-ordinator for North East London Deanery.Mrs. Pisal is known for her expertise, kindness and her willingness to go out of her way to provide the best possible care for her patients.
For general Enquiries
020 7580 4400
9am - 5pm Monday to Friday
Email contact centre
Send us an email and
we will get back to you.
Click here to register
The Portland parenting magazine
Download your free copy
Meeting cultural needs
Find out more
Antenatal Classes
© 2007 - 2017 The Portland Hospital.
Web Design Agency - Brandcast Media
The Portland Hospital UK, dedicated to the healthcare of women and children.


text:
One Fine Day
Together, warm, safe
Sweet heaven, cascading light
Rolling fields, bright sky.
Well, that's not going to be 140 characters long, is it. Actually, with the HAIKU!s it's 80ish (not including spaces) so i wonder.... maybe with genius word useage, it might be possible to get to 140... maybe.


text:
Home >> 20SP Air Pneumatic Quick Coupler Joint Connector Fittings for 5mm x 8mm Pipe
|Product Name
|Air Quick Coupler
|Connector No.
|Fit Pipe Size
|5 x 8mm/ 0.19" x 0.31"(Inside Dia*Outside Dia)
|Other End Inner Dia
|13mm/ 0.5"
|Overall Size
|50 x 22mm / 2" x 0.8" (L * Max.D)
|Silver Tone
|1 x Air Quick Coupler
- Nickel-plated metal construction for greater durability, ensure reliable connection.
- Connect equipment conveniently with Quick Coupler.
- Perfect for use with high air flow equipment and air compressors.
This Quick fittings are only used for industrial automatic apparatus and can not be applied on any medicinal equipment.
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text:
Never give up- There is still much more too do!
Well I'm here to tell you don't give up and get going making a change! That's right, don't throw in the towel yet, it's time to push forward. Don't allow defeat to stop you from being all you can be and enjoy the life you want, the life promised to you by God.
A month ago, I was talking with a dear friend, venting about school and how I'll be glad when I'm done. All of a sudden there was a silence came over the phone; "are you there I asked? Her reply was yes, "I just want you to hear yourself and realize that you are at the finish line and doing better than you see". You are tired she said; you been at this for the last 3.5 years as well as trying to keep things going with your business and life. You are almost there!
I know that you tired; things are not working, every time you try something it's either put you further behind or cause destruction to what has already destroyed. OR! You are on a roll and BOOM! Things come to a halt, all the customer stop, the meeting are no longer on your calendar and you are going "what happen?" Do you give up? Do you revise, reinvent, or do you believe you are done? I love basketball; I loved the sport since the 7th grade. I like the action on the court and the drive the players have to win the game. So, having the pleasure to work for the Dallas Mavericks basketball team in their fan shop has been a dream come true. Now working in the fan shop; that is not my dream come true, but working for Maverick's is. Why, because working in the fan shop puts me closer to the business I want to do with them. I'm not giving up.
Don'ts let age, money or even people discredit you or discourage you from moving forward. If you're ready for a promotion, go for it
If your goal is to drive the Pink Cadillac go for it
If your dream is to bake the best German Chocolate cake in the world and sell it… Go for it
The only person who can make your dream come true; change your situation moving you in the direction you want to go is you.
A dear friend of mines informs me that she started her Mary Kay business. Soon Latesha Mosely was wearing a red jacket, built a team and was taking off in the direction to get her free car! I'm quite sure there were days she faces with no sells or her team not reaching their goals, but each time I see her she is making it happen.
Rome wasn't build in a day I was told, but that didn't stop the builders. I encourage you to do you and that means doing what it takes to be the best in your job, career and business. Don't give up on you, because God hasn't given on you yet!
How did you move forward to over come challenges? What made you not give up?
I would love to hear your comments!!
Until next time.. The Business Woman


text:
Currently 67.5 °F
May 29th, 2017
5:50PM on Friday 18th July 2014
( 2 years ago )
Federal judge sends key GM case to Ga. court
The Associated Press
Contact Editor
DETROIT (AP) -- A federal judge in Atlanta has sent an important wrongful death lawsuit against General Motors back to a Georgia court.

 Judge Thomas Thrash Jr. ruled Friday that a Cobb County judge should handle the case filed by the family of Brooke Melton. GM had moved the case to the federal court system.

 Melton, a 29-year-old nurse, died in a 2010 car crash near Atlanta. Her parents sued GM alleging that a faulty ignition switch in her 2005 Chevrolet Cobalt unexpectedly shut off the engine, causing her to lose control of the car.

 The case exposed that GM knew about the faulty switches for more than a decade but failed to recall the cars until this year. It also touched off a crisis at the company that has resulted in 54 recalls involving 29 million vehicles this year. And it brought federal investigations, cover-up allegations and a $35 million fine from federal regulators for delays in reporting safety problems.

 "It has to be a bad day for General Motors," said Jere Beasley, a Montgomery, Alabama, lawyer who is on the Meltons' legal team. The move to a local court, he said, is likely to speed up questioning of company executives and employees under oath. A local jury also is more likely to issue strong punitive damages if it finds GM at fault, he said.

 Beasley said he and Lance Cooper, a Georgia lawyer who filed the original case, plan to file motions on Monday asking the judge to allow depositions of GM CEO Mary Barra, Corporate Counsel Michael Milliken and all 15 employees that were dismissed by GM due to the ignition switch case. They'll also ask to question Ray DeGiorgio, the engineer who designed the switch, and all of his supervisors.

 "I think the key to the overall General Motors litigation picture is taking depositions under oath of key people," Beasley said.

 A GM spokesman said the company would not comment on pending litigation.

 Even though the case was sent back to a local court, the Meltons still have to convince Cobb County Judge Kathryn Tanksley to reopen it, which judges normally frown upon. They settled last year with GM for $5 million, but are willing to return the money and take the case to trial.

 To reopen the case, lawyers must prove that GM fraudulently concealed evidence. But Beasley says he's confident they'll be able to do that.

 In a deposition before the case was settled, DeGiorgio said under oath that he didn't make any changes to the ignition switches after they went into production. But a document provided to Congress by switch maker Delphi Automotive showed that in 2006, DeGiorgio signed off on changes that made the switches harder to turn. GM did not produce the DeGiorgio document in the Melton case.

 Cooper contends that DeGiorgio lied under oath and that GM covered it up. Repeated efforts by The Associated Press to reach DeGiorgio have been unsuccessful.

 The Melton case eventually forced GM to recall 2.6 million older small cars to replace faulty switches. The switches can be turned too easily and can slip out of the "run" position unexpectedly, shutting off the engine and knocking out power steering and brakes. That can also disable the air bags.

 Beasley said that if lawyers can successfully reopen the Melton case, it likely will be the first to go through the pretrial discovery process. Anything unearthed by lawyers in this case likely will be used in future lawsuits against GM, he said.

 GM has acknowledged that at least 13 people have died in crashes liked to the switches, but lawyers and lawmakers say the death toll is closer to 100.
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text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"Stepan Mishura" <stepan.mish...@gmail.com>
|Re: [classlib][security] Compatibility issue for UnresolvedPermission.getUnresolvedCerts()
|Tue, 09 Jan 2007 09:27:08 GMT
On 12/29/06, Tim Ellison wrote: > > I've been sat on HARMONY-734 for a while (for which I apologize) -- so > bringing it here to see what the opinion is about following the spec vs. > being compatible with the RI that doesn't follow the spec. > > Summary is, where there are no certificates the method: > java.security.UnresolvedPermission#getUnresolvedCerts() > > returns an array of nulls on the RI, but > returns null on Harmony, as required by the spec. > > Question is, would apps be dealing with null as a return value, or will > following the spec in this instance cause problems? Hi Tim, I think the issue should be marked as non-bug difference - the class is used to hold unresolved permissions when security policy is initialized. The unresolved permission are resolved later during access control checking. This design doesn't imply that an app. deals directly with an array of unresolved certificates. But if it does I can not image a reason for policy implementation to initialize a permission object with an arrays of nulls. So I don't see any potential problems for apps here and I'd follow the spec. Thanks, Stepan Mishura Intel Enterprise Solutions Software Division
|View raw message


text:
Infuriating doesn't even begin to describe this
If you're reading this and you happen to be a white male, congratulations. You've won a lottery that has been around for, oh, around 200,000 years. Your prize? Privilege and a virtual "get out of anything" card.
Women and minorities have been fighting for the same rights as our western/european counterparts for hundreds of years, and while we have come a long way, we have an extremely long way to go. Unfortunately, in America, if the horrible excuse for a man we call Donald Trump wins the election, we might retreat in rights for four years, and, who knows, a girl might get raped without almost no consequence to the perpetrator…oh wait. That already happened.
Five men have sexually assaulted a woman for the second time in Rotak, India and are facing mild to no punishment for their actions.
Violence against women is getting noticed, but not stopping.


text:
By Unofish$119.00 $249.00
Empires established here. They aren't built in a day and it's in that spirit that Unofish presents the Gold Rush Basketball, an ode to one of the premiere franchises in football. We start by handcrafting a python embossed gold leather and pair it with Italian red tumbled leather. It's a luxury exemplary of the Faithful it represents. Who's got it better than us?
- Handcrafted basketball made from rich Italian and imported leather
- Italian Red Tumbled Leather
- Python-Embossed Gold Calf Leather
- Black Polyurethane Rim
For every product sold, Unofish donates a premium composite basketball to a child in the community. Learn more.


text: Bring the Vote Home specifically focuses on Medicare beneficiaries because they must be homebound to be eligible for Medicare's home healthcare benefit, which makes it challenging for seniors to travel to polling locations. Ohio's home healthcare community represents 115,000 Medicare home health beneficiaries and more than 17,000 skilled home health clinicians.Nearly 3.5 million Americans nationwide currently receive Medicare home healthcare services for illnesses related to acute, chronic or rehabilitative needs. Data show skilled home healthcare is a clinically advanced, cost-effective and patient preferred option for American seniors. Full poll results available upon request. Bring the Vote Home is a nationwide citizenship project coordinated by the Council for State Home Care Associations , Partnership for Quality Home Healthcare , and other home health community members to help America's approximately 12 million home healthcare patients, including 3.5 Medicare beneficiaries, and skilled healthcare professionals exercise their right to vote in national and state elections this November. To learn more, visit www.bringthevotehome.org. SOURCE Bring the Vote Home


text:
Clarkson being a medium-sized school, "you meet tons of people and get to form good connections with most," and the excellent alumni network is buoyed by the many internships and co-ops that are readily available. "I know that if I obtain a degree I will have little to no trouble getting a job," says a civil engineering major. Facilities are the source of many complaints here, as "nearly all of the academic and recreational buildings could use a major overhaul."
For a STEM major there is "much pressure put on you by the workload," so students study for the most of the week—"sleepless nights are commonplace and being in more clubs than you should be able to handle is normal"—then decompress with "sports, clubs or other activities in their free time." There is "a good attempt at late night programs by res life, but often times you'll end up at a party." Greek life "thrives on weekends" and the Division I hockey games are "a great event where the whole community—both school and town—gather together and cheer as loudly as possible."
The Jeanne Clery Act requires colleges and universities to disclose their security policies, keep a public crime log, publish an annual crime report and provide timely warnings to students and campus employees about a crime posing an immediate or ongoing threat to students and campus employees.
Please visit The Princeton Review's page on campus safety for additional resources: http://www.princetonreview.com/safety
The Princeton Review publishes links directly to each school's Campus Security Reports where available. Applicants can also access all school-specific campus safety information using the Campus Safety and Security Data Analysis Cutting Tool provided by the Office of Postsecondary Education of the U.S. Department of Education: http://ope.ed.gov/security


text:
Recovering from depression isn't a smooth one way process. There's lots of relapses. Relapses are okay. Relapses are part of depression. They are warning signals that you might be pushing yourself too much emotionally, mentally, physically, or spiritually. Relapses can be nasty, not just for you but people you care about. Having a good depression relapse safety net in place can limit the severity and duration of your relapse. Here's 5 ways to prep for a depression relapse.
The holiday season is a notorious time for unwanted weight gain. Office parties, family gatherings, and a thousand temptations wherever you turn can quickly lead to extra pounds that are hard to get rid of in the aftermath. Fortunately, none of this is inevitable, and you don't have to wait until New Year to get back on track.
Christmas is a time for traditions, togetherness, recounting memories of Christmas past, and of building new memories. However, Christmas also includes an undeniable commercial element! With just over one week until Christmas, here are some 2013 Hot Gift suggestions that will make Holiday shopping simple for the young and old on your list!
It's not a new concept for builders to offer incentives in the form of upgrades to potential buyers. However, I've been astounded by other incentives that have recently popped up in the Toronto real estate market. For example, Kylemore Communities has been offering $35,000 towards any new BMW, with a purchase of one of their specific condo developments.
What do you really need for a successful year of college or university, aside from enthusiasm, smarts and inspiration? Text books are an obvious answer, but there are some important basics and creature comforts that should not be overlooked. Read below to see 10 back-to-campus essentials for September!
On January 1 I decided to make some major changes to my family's nutrition and physical activity. I began to think of our five young children, all the proper body weights--but with a frequent propensity to eating desserts and exercising too rarely. They also learned by example, and noticed when I would snack.
When I was a child in the 1970s and 80s, there was a plenitude of catchy commercials and singable jingles! Many of these commercials had enough staying power to last a lifetime, and have been permanently imprinted on my memory. Here's a list of the best 10 commercials and jingles that I remember from my childhood, in no particular order.
If you're looking to save some money this holiday season, did you know that there are some great deals to be found at the Dollar Store? Although some items on this list may not be found at your particular Dollar Store, many of them will be! Save dollars at a time when you shop the items from this list!


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30 Gospel Classics [Gusto] [CD]
- Artist: Various Artists
- Release Date: 07/11/2006
Best Buy is dedicated to always offering the best value to our customers. We will match the price, at the time of purchase, on a Price Match Guarantee product if you find the same item at a lower price at a Designated Major Online Retailer or at a local retail competitor's store.Here's how:
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- Jesus Loves Me
- He's Got the Whole World in His Hands
- Ave Maria
- Swing Low Sweet Chariot
- Rock of Ages
- I Love to Tell the Story
- Whispering Hope
- Onward Christian Soldiers
- Faith of Our Fathers
- Blessed Assurance
- Wayfaring Stranger
- Kum-Ba-Yah
- Peace in the Valley
- Holy, Holy, Holy
- Let Us Break Bread Together
- A Mighty Fortress Is Our God
- Doxology (Praise God from Whom All Blessing Flow)
- The Old Rugged Cross
- In the Garden
- What a Friend We Have in Jesus
- Battle Hymn of the Republic
- In the Sweet By and By
- Amazing Grace
- Farther Along
- Softly and Tenderly
- Life's Railway to Heaven
- His Eye Is on the Sparrow
- Unclouded Day
- Abide with Me
- Church in the Wildwood


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A six-year-old boy has been killed after crashing his mini-motorbike into a tree.
Kane Small, from Bloxwich, West Midlands, died after the vehicle he was riding in a nature reserve crashed on Sunday.
The youngster was flown by air ambulance from the scene of the incident, near the Arrowcraft Marina, to Birmingham Children's Hospital, where he died.
Kane's funeral will take place on July 17 at All Saints Church in Bloxwich.
His family issued a statement inviting anyone wishing to pay their respects to attend the service.
They said their son was "infatuated" with mini motorbikes, and had been riding a friend's vehicle when the incident happened.
The statement added: "We would like to thank the many people of Bloxwich and in particular the Mossley Estate for all the many floral tributes, and help and support that has been received during this sad time."
His parents also called for more funding to be given to local authorities so organised mini motorbike tracks can be created.
Scroll down for more
The statement added: "We believe consideration should be given to funding organised tracks through the local authority or lottery grants so that ordinary people can take their children to use their motorbikes in a safe and supervised place."Reuse content


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You missed it This great deal for Stroller Strides is over. But don't despair, we've got more great deals every day.
Related Deals That You May Like
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The lone-wolf routine may be suitable for the occasional stroll with your cub, but going it alone doesn't make for the most exciting or efficient workout. Join a pack of parents with today's deal from Stroller Strides: Pay $25 to track down ten stroller-based exercise classes (a $75 value). Designed for new moms of any fitness level, this fierce program combines power walking with interval training to improve your strength and stamina. You'll tone your muscles using exercise tubing, the stroller, and the world around you -- all while engaging your little one with songs and activities. With locations at the Bethany Lutheran Church and Westfarms Mall, you don't have to travel far from your den to lose weight and have fun with other moms who will make you proud to be in their pride.
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Bethany Lutheran Church
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Eastin Center for Modern DentistryDentistry
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ANALYSIS: World War III Is Being Rehearsed by Over Three Dozen Nations
ANALYSIS: World War III Is Being Rehearsed by Over Three Dozen Nations If it quacks like a duck and walks like a duck, then it is a duck. If dozens
Canada Sells Weapons to State Sponsor of Terrorism: Class Action Law Suit against Ottawa over $15 Billion Saudi Arms Deal
The Canadian government of Prime Minister Justin Trudeau is the object of a class action lawsuit in Quebec Superior Court pertaining to the $15 billion sale of weapons to Saudi
ANALYSIS: Chipotle Is A Victim Of Corporate Sabotage – Biotech Industry Planting E-Coli In Retaliation For Restaurant's Anti-GMO Menu
ANALYSIS: Chipotle Is A Victim Of Corporate Sabotage – Biotech Industry Planting E-Coli In Retaliation For Restaurant's Anti-GMO Menu (NaturalNews) After observing recent events involving Chipotle and e.coli, here's my
Will There Be A New False Flag Attack In Chicago's Sears Tower?
The Excavator By Saman Mohammadi Originally Published on May 30th, 2011 "Look for a false flag," said Celente, "look for something to rally the people around the president. They'll do
Daniel Ellsberg: "Secrets … Can Be Kept Reliably … For Decades … Even Though They Are Known to THOUSANDS of Insiders"
Judges, prosecutors and journalists all know that collusion happens every day. As I noted last year: Bernie Madoff's Ponzi scheme was a conspiracy. The heads of Enron were found
Memory Hole – Obama Officialy Authorizes Covert War To Overthrow Syria
President Obama has signed an order that authorizes the CIA and other US to provide Syria terrorists the support they need to overthrow the Syrian government. Obama Officially Authorizes Covert
Governments ADMIT That They Carry Out False Flag Terror
Since ancient times governments have committed false flag terror attacks to push political agendas – Here's a list of admitted recent occurrences. Preface: Please skip to the end of this
Why Do Progressive Liberals Fall For 'Humanitarian War'?
The concept of 'Humanitarian' War contradicts 200 years of liberal thought and the ideals of our founding fathers yet progressives still fall for it. Why Do Progressive Liberals Fall for
False Flags In Syria: An Operation 50 Years In The Making
Examining historical evidence which suggests the false flag operations going on in Syria is a U.S. government operation that is over 50 years in the making. Don't believe all of
Secret NYPD Cell Flushed Out By 911 Anti-Terror Call (*9-1-1 Audio Included*)
Newly released 911 audio recordings give insight into how the FBI flushed out a secret NYPD cell following an anti-terror call. Rushing to the scene, the FBI found a small


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text: Divxturka.net is using Redbunker! Because Redbunker is better than Hotfile, Netload and Rapidshare ! Click here and buy REDBUNKER premium account! Plot: Tyler Perry's new musical stage play starring the infamous Mabel Simmons or "Madea" as her fans know her. When a judge orders Madea to do 20 hours of community service at a local retirement home the residents and staff are not ready for Madea's brand of "the truth", but all is well that ends well when Madea helps the residence of Easy Rest Retirement Home realize the importance of family, love and forgiveness Genre: Comedy | Musical IMDB Rating: -/10 Directed by: N/A Starring: Tyler Perry Size: 1.29 GB Video: AVi Audio: English Runtime: 02h:22m:31s Information: Code: http://www.amazon.com/Tyler-Perrys-Madea-Gets-Job/dp/B00AJLHVES Buy Redbunker Premium Account & Support me to post more stuff for you. Download From Redbunker Code: http://www.redbunker.net/edl3makkykdt/Tyler-Perry-s-Madea-Gets-a-Job-The-Play-2013-DVDrip-XviD---MATiNE.avi.html


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Distribution and Poverty Impacts of Tax Structure Reform in Developing Countries: How Little We Know
The genesis of this article is a study commissioned by DFID in 2000; a longer and more detailed version of the article is available in Gemmell and Morrissey (2003). The authors are grateful to two anonymous referees for suggested editing and revisions. The views expressed are those of the authors alone.
The past two decades have witnessed widespread reforms of tax structures in developing countries. This article reviews available evidence on the effects of various taxes, and hence of tax structure reform, on distribution and the poor. Taxes on exports and goods consumed especially by the poor (e.g. kerosene) are the most consistently found to be regressive, whereas taxes on 'luxury' items such as cars, beverages and alcohol are the most likely to be progressive. Sales taxes are slightly more progressive, or less regressive, than taxes on imports. The reforms implemented are therefore unlikely to have worsened the effects of the tax structure on the poor.


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Dragon Hotel
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
TravelPod Member ReviewsDragon Hotel Melaka
The family room we took were spacious and the location is walking distance from the main shopping area, the pahlawan mall, Jonker street, etc. in the evening right at the street level below are the full stretch of night market which makes it such a convenient place to stay at...
This review is the subjective opinion of a TravelPod member and not of TravelPod.com.
Travel Blogs from Melaka
Chelsea's first blog: A day in Malacca
... patient person I am, gently tapped on the door and rang the bell once or twice, to unfortunately find no response!!! After standing there like lemons for 15/20 mins, we examined the confirmation email to find we had to check in else where. You can just imagine how enjoyable I found all of this! When we got to the next door, a few more gentle taps and a cheerful lady told us we would be staying in a different hotel!! After I left Rob to calmly pay the lady we checked into ...
Visiting Malacca – 1st time through the stra
We spent yesterday at sea, sailing around the bottom of the Malaysian peninsula toward the west and up through the Malacca Straits, fabled shipping passage, and past Singapore (we'll be back later) on our way to Malacca, Malaysia. Today, we visit Malacca - a UNESCO World Heritage site since 2008. The city was founded in 1403 by the Portuguese and changed hands many times over the centuries – the Dutch controlled the area for some time and we visited Dutch Square ...
Jonker Street
... with your hand (that is without cutlery), even by the smartly dressed business people and well-to-do families around us. Ben followed their lead and thinks he didn't make a fool of himself. The afternoon involved a slow walk about the old city. The heart of the heritage district is the Dutch square with 1700s Christ Church and the grand Stadhuis, the oldest Dutch building in Asia. In the middle of the square, there is an elegant memorial fountain to Queen ...
Melaka, Mellaca, Mallaca
... at the river and over the small bridge is the UNESCO area. We explored that area visiting the ruins of St. Paul's Church, the recreation of the sultanate palace and gardens, the Dutch Cemetery and took in the views of the Straits of Melaka.
One evening, we took a river boat ride and saw the sites from the water. That was nice and quite a deal at $5 each for the 40 minute trip. It started pouring near the end, but we were able to take ...
Marvellous Malacca, not just another UNESCO town
... left Melaka for the Islamic port nearby in Java. In 1641 the Dutch ruled, re-establishing the area and leaving their mark on the city. Then in 1975 when the French occupied Holland, Britain took charge of the Dutch colonies placing their slant on things. The result is a vibrant architectural dream. A slender mix of Chinese-Malay, Dutch and British colonial charm with a heavy Portuguese presence still felt (even in the food!). Melaka with Penang and Singapore became ...
Other places to stay in Melaka
Hotel Equatorial MelakaJalan Bandar Hilir, Melaka | 4 star hotelfrom $78
Ramada Plaza MelakaJalan Bendahara, Melaka | 4 star hotelfrom $76
- Mariott
Mahkota Hotel MelakaNo. 16 Jalan Syed Abdul Aziz, Melaka | 3 star hotelfrom $47
Bayview Hotel MelakaBayview Hotel Melaka Jalan Bendahara, Melaka | 3 star hotelfrom $56
Everly Resort10km Jalan Tanjung Kling, Melaka | 4 star hotelfrom $116
Golden Legacy Hotel Melaka146 Jalan Hang Tuah, Melaka | Hotelfrom $42
A'Famosa Resort Hotel MelakaVersailles, Melaka | 4 star hotelfrom $96
Grand Continental MelakaNo 20 Jalan Tun, Melaka | 3 star hotelfrom $45
Emperor Hotel MalaccaMalacca Hotel 123 Jalan Munshi Abdullah, Melaka | 3 star hotelfrom $43
The Majestic Malacca188 Jalan Bunga Raya, Melaka | 5 star hotelfrom $138
Fenix Inn156 Jalan Merdeka, Melaka | Hotelfrom $28
- Fenix Inn Melaka
- Good
Hotel PuriHotel Puri Jalan Tun Tan Cheng Lock, Melaka | Hotelfrom $49
- Mooi koloniaal hotel
- Restful residence
The Baba House125-127 Jalan Tun Tan Cheng Lock, Melaka | 2 star hotelfrom $39
- Basic, but value for money
- Good budget choice
- Not deserve the price
Hallmark HotelHotel Hall Mark 68 Jalan Portugis, Melaka | 3 star hotelfrom $30
- :)
Accordian HotelAccordian Hotel 114A Jalan Bendahara, Melaka | 3 star hotelfrom $28
- satisfied
Aldy Hotel27 Jalan Kota, Melaka | Hotelfrom $50
Mimosa HotelMimosa Hotel 108 Jalan Bunga Raya, Melaka | Hotelfrom $34
Seri Costa Hotel-ResortHotel Seri Costa Jalan Pm 8, Melaka | Hotelfrom $35
- Good for families!
Hotel Orkid MelakaHotel Orkid 138 Jalan Bendahara, Melaka | Hotelfrom $42
Malacca Straits HotelNo. 37A Jalan Chan Koon, Melaka | Hotelfrom $41
Atlantic Park Hotel9830 Bukit Baru, Melaka | Hotelfrom $17
Klebang Beach Resort92-1 Km9 Batang Tiga, Melaka | Hotelfrom $50


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Item Specifications...
Create pathways in theological education and congregational practice for people with disabilities
Item Description...
Graduate Theological Education and the Human Experience of Disability examines graduate schools of theology and their limited familiarity with the study of disability--and the presence of people with disabilities in particular--on their campuses. Dubbed a "missing note" by one theologian, this text offers critical research and illuminates new pathways for "theologia" and practice in the community of faith. Reviews of previous literature, theology, and practices illuminate how people with disabilities have historically been marginalized by the religious community. Theologians, people with disabilities, and researchers offer suggestions for incorporating disability studies into theological education and religious life.
This text contains firsthand testimony from people with disabilities who are the necessary sources of wisdom for overcoming barriers. By infusing education into existing theological curriculum, seminaries may better prepare their students for leadership and ministry in their congregations. People with disabilities number 18% of the population, yet represent only 5-7% of congregational membership.
This book explores aspects of theology and disability such as: the challenges faced by theological schools that desire to improve both theological curriculum and facilities a review of literature that connects theology and disability--from sources such as scripture, history, faith traditions, and social theory the various ideologies that shape the way the human body is understood--redefining "normal" in theological education an overview of critical boundaries that mark the limits and possibilities for theological inquiry about the human experience of disability creative concepts that religious communities may use to better include people with disabilities and their families how the religious community may benefit from the gifts, talents, and leadership of people with disabilities Graduate Theological Education and the Human Experience of Disability contains a reprint of Dr. Harold Wilke's landmark 1978 article from Theological Education (published by the Association of Theological Schools). Dr. Wilke, born without arms, was the theologian, minister and scholar who first articulated the need to address the human experience of disability in both theological education and congregational life. With extensive biographies and inclusive liturgies, this innovative text is a valuable resource for seminary professors and leaders, clergy, and disability advocates.
Buy Graduate Theological Education and the Human Experience of Disability by Robert C Anderson from our Christian Books store - isbn: 9780789060105 & 0789060108
The team at Christian Bookstore .Net welcome you to our Christian Book store! We offer the best selections of Christian Books, Bibles, Christian Music, Inspirational Jewelry and Clothing, Homeschool curriculum, and Church Supplies. We encourage you to purchase your copy of Graduate Theological Education and the Human Experience of Disability by Robert C Anderson today - and if you are for any reason not happy, you have 30 days to return it. Please contact us at 1-877-205-6402 if you have any questions.
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731-735 Darling Street
Rozelle NSW, 2039 Rozelle
Phone: (02) 9555 8300
Email: email@example.com
The Essential Ingredient Rozelle cooking school, proudly sponsored by St George Appliances, offers a variety of cooking classes in a relaxed & friendly environment, highlighting the joy of sharing the passion for food with friends and family.
Classes range from basic knife skills where enthusiastic foodies learn to sharpen, slice & dice a selection of fresh produce, to a range of regional specialty classes such as, Spanish, Moroccan, Malaysian, Indian, Asian & Italian.
Allow your taste buds to be taken on a journey through the mouth watering selection of classes with dedicated local and international chefs.
For more information, or to book any of these classes, call (02) 9555 8300.
For corporate enquiries, please call Kate Evans on (02) 9555 8300 or send an e-mail with your event details to: firstname.lastname@example.com
Cancellations are not refundable.
All bookings are confirmed on receipt of full payment and if a seat is available.
If you cannot make it to your reserved class, you are welcome to send someone in your place.
The Essential Ingredient reserves the right to alter or cancel a class without notice.
Please inform staff of any dietary requirements when making a booking. We cannot accommodate dietary requirements in all cases.
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cooking classes, cooking class, cooking school, cooking schools


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Speed up your debug routine!
Automated exception search integrated into your IDE
Tired of useless tips?
Unknown lifecycle phase ",,,,**apache-ant/src/tests/**/*,apache-ant/src/etc/testcases/,,,**/resources/**/*,,,,,". You must specify a valid lifecycle phase or a goal in the format <plugin-prefix>:<goal> or <plugin-group-id>:<plugin-artifact-id>[:<plugin-version>]:<goal>. Available lifecycle phases are: validate, initialize, generate-sources, process-sources, generate-resources, process-resources, compile, process-classes, generate-test-sources, process-test-sources, generate-test-resources, process-test-resources, test-compile, process-test-classes, test, prepare-package, package, pre-integration-test, integration-test, post-integration-test, verify, install, deploy, pre-clean, clean, post-clean, pre-site, site, post-site, site-deploy.
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text:
Three days later, a text beeped onto my phone:
You have (1) new message:
From: Vicky
Hey babee. Sorrii bout wat hapend b4. hopin were stil m8s? cz ur realli makin me sad...
How dare she use her unstable nature against me? But I smiled as I read the message. At least she had the decency to apologize, even if it was delayed three days. I wasn't going to give in that easily though.
Sorry? Rite, Ive heard dat one befor. Why do you need me as a frend anyway? Youve got milly an harriet. dont need me.
Message Sent
You have (1) new message
From: Vicky
wtf? kate, uve bin ma bst m8 4 years wat makes that change now?
You are what made that change. You go gallivanting off with milly and harriet every day and dont spend a thought for me, your real frend whos been with you through evrything. i dunno why i bother still bein your frend. just leave me alone - deal with your problems yourself and dont drag others down with you.
Message deleted
You're right but I still want to talk to you properly. Can you go on msn?
Message sent
Why was I giving her this chance? I was too weak-hearted I suppose. So I logged on to my computer and within a few minutes, the announcement that EmoKid had signed on popped onto my screen.
EmoKid: Heyy wat did u wanna sayy?
BlueEyedGirl: Just that I hope you are ok with what i sed to you.
EmoKid: Sure I get it all da time - hey what lessons have we got tomorro?
I sighed. Using me for school help. Again.
BlueEyedGirl: cba checkin. geography an maths an three others lol are we friends?
EmoKid: Of course
Somehow I wasn't so sure.
A few days later on our first day back at school, Vicky once more had slashes along her wrists. But I bore my patience. I had debated with myself over the course of a few days and I decided that even though I may not be her best friend, she was probably mine and I should support her. Earn her trust. After all, I was the one she'd end up crawling back to.
Over the next week, I helped her. I was there when she was in tears and no-one else could comfort her. And my plan worked. All of a sudden, she was spending more time with me and not Milly or Harriet. I was the one she asked to go into town first. I was her best friend.
And I was ok with it.


text:
The Round and Other Cold Hard Facts
€ 19,49
Lieferbar innert 2 Wochen
September 2002
BeschreibungSet largely in locations along the French Riviera, these 11 short stories depict with great compassion the harsh realities of life for the less-privileged inhabitants of this privileged region.
PortraitWinner of the 2008 Nobel Prize for Literature, J. M. G. Le Clezio was born in Nice in 1940 and is one of France's best-known contemporary writers. He has published more than thirty novels and nonfiction works. In the course of the last four decades Le Clezio has won numerous prizes, including the Prix Renaudot for his first novel. His works have been translated into many languages. C. Dickson is a translator living in France. Her translations include Shams Nadir's The Astrolabe of the Sea and Mohammad Dib's The Savage Night (Nebraska 2001).
Pressestimmen"Le Clezio is an intensely atmospheric, nearly hallucinatory writer, and in his riveting and eviscerating short stories, dreams turn inexorably into nightmares."--Booklist Mentioned in The New York Times (Paperback Row) December 28, 2008
Untertitel: black & white illustrations. Sprache: Englisch.
Verlag: University of Nebraska Press
Erscheinungsdatum: September 2002
Seitenanzahl: 214 Seiten
Übersetzer/Sprecher: Übersetzt von C. Dickson
Format: kartoniert


text:
According to Azerbaijani State Oil Company (SOCAR), in November 2014 SOCAR transferred to the state budget 142,373,000 AZN.
During January-November 2014, SOCAR has transferred to the state budget 1,706,921,000 AZN. SOCAR's payments to the state budget has increased by 219,509,000 AZN or 114.8% against the same period last year.
In November 2014, the company's payments to the State Social Security Fund totaled 10,618,000 AZN.
From January to November 2014, SOCAR's payments to the Fund totaled 129,534,000 AZN.


text:
Product Information
**Please note: We do not take returns of costumes after October 15.
Halloween will bring fun for your sweet girl in the Pink Princess Halloween Dog Costume!
Why We Love It:
The Pink Princess Halloween Dog Costume will look adorable on your royal pup. Two piece costume, includes a corset top with tulle on the skirt, pink dress with attached lace petticoat, gold trim and adjustable drawstring headpiece. Easy to wear front closure.
Important Sizing Note: The following measurements are the dimensions of the actual product, not of the pet they fit. After measuring your pet, make sure all measurements fit within the following dimensions (with a little room to spare in the chest and neck).X-Small


text:
First Blood of the Day-Part 2Mature
And then he ran. Titus sprinted towards his opponent with deathly determination in his eyes.
"What is your man doing, Octavius?" Sextilius said. Does he not know that the fighting begins once I say it does, he thought.
"You cannot blame him for being eager, can you? He is but excited as this is his first battle in the arena." Octavius smirked.
Turning around and seeing Titus fly at him, the opposing gladiator charged. Titus swung both swords in an arc towards his enemy's head, but his shield easily knocked the blades aside. His opponent threw himself forward ramming his steel plate into Titus's chest, knocking him to the ground. Seeing the sword coming towards him, Titus rolled out of the way as his enemy tried to stab him in the heart. When the tip of the sword hit the sand Titus jumped up and saw his opportunity. He flung his blade and while his enemy deflected it, he rushed ahead tackling him, digging his knee into his stomach. Knocking the breath out of him, Titus commenced to beat him with the wooden pommel of his sword, at first breaking his nose. The gladiator looked back at Titus with horror as he felt his nose crack under the weight of the wood. Holding him down, Titus reigned down blow after blow, each one bloodying his enemy's face and making it more and more unrecognizable. Anger burned in his eyes as he destroyed his opponent's features, watching him bleed profusely. Titus hit harder with every attack and he heard a sickening crack as he crushed the gladiator's skull. Finally, after completely caving in his enemy's head like a broken cask of wine, he stopped and stared at the bloody mass of splintered bone and gore.
The crowd shouted their approval as they saw Titus slaughter his adversary. Like a flock of hungry vultures, they came for blood and death, and they received it in abundance.
Getting up from his seat, Octavius leaned over the balcony and started to applaud and cheer with the audience. "Citizens of Rome! The gladiator that just eradicated Sextilius's man is named Titus! He is my Champion!" he screamed to the crowd. They shouted their approval and starting to chant Titus's name. "Would you like to raise the wager further?" Octavius asked Sextilius while admiring the crowd praise his champion.
Attempting to not show any irritation in his voice, Sextilius said "You think because you've killed one of my men that the match is already yours? There are still three more fights to go until a winner is decided."
"I recall asking you a similar question just moments ago. You seemed certain that you would be the victor, even after just one fight. How certain are you now, Sextilius? Certain enough to bet 100 denarii that you will rise victorious?"
Liviana gasped at her husband's offer and whispered "Octavius, 100 denarii? You cannot be serious!"
"Liviana, my sweet, this fuck doesn't have enough man in him do it anyway" Octavius said just loud enough so Sextilius could hear.
Provoked enough, Sextilius yelled "I will not be fucking spoken to in that manner! You think I will not bet 100 denarii? I'll bet every one of my fucking men and all of my riches! Will you do the same?" he asked even though he hoped desperately that Octavius would decline.
"I will consider your offer as betting does not frighten me, and nor do you. Now excuse me a moment, I would like to have a word with my gladiator. Relax and keep my wife company. I hear that you love to gossip with the women."
Sextilius was fuming with anger, his face flushed red with rage. He wanted to say something else to Octavius, but before he could Octavius left the balcony. He met Titus down in the holding cells under the arena. Passing the decomposing dead bodies, he found Titus sitting on a bench with his head down, awaiting the next fight. Octavius went up to him and said "That's how you send someone to the underworld, Titus! That pissy little shit Sextilius might as well had woken up to his own severed cock in his mouth!"
"I'm glad you're pleased, Dominus." Titus said looking his master directly in the eyes. He was grateful for being given the chance to fight in the arena and truly wanted to make his Dominus proud. He was determined to become the best gladiator Octavius has ever seen and make the House of Octavius Crassus become legend.
"Pleased? More than pleased, Titus! Did you see the crowd? They cheered for you Titus! For you! When you're fighting in the arena you stand as a God. The arena gives you a chance to rise up above the heavens! And that is what you did today. From this day onward, no one will ever forget your name. When we win this fight, you will be on the tongues of every citizen in Rome! I will reward you with wine and women, any women of your choosing! And Titus, show no mercy. Understood?"
"Yes, Dominus."
"Good. Ready yourself for another battle." Octavius patted Titus's shoulder and looked him in the eye. With a voice like ice he said "None of his gladiators live. No matter if they give the sine missione, they die. You will kill all of them." And with that Octavius left Titus to go back into battle.
When he arrived back to the balcony he saw Sextilius still furious. At an attempt to further enrage him he went over to Liviana and kissed her, surprising her. He grabbed the back of her head and continued to kiss her, their tongues dancing in each other's mouths. Her scarlet lips tasted like the honeyed wine that she was drinking, her golden hair smelled like sweet perfume, and the combination of both intoxicated him. Her silky skin against his made him kiss her harder, and they moaned in each other's mouths. It seemed like hours before they finally ended their embrace. Octavius and Liviana looked over to see Sextilius staring at them, his mouth open in astonishment. Liviana blushed, quickly looking away and taking a sip of wine while Octavius laughed and said "I accept your wager."


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A Guy Pranks Strangers by Making Them Think They Have Ridiculous Stuff in Their Hair . . . Like a Full Loaf of Bread [Video]
A group of guys who make prank videos have a new one where one of them goes up to random people, and says they have something in their hair. Then he offers to grab it for them.
But when he goes to grab it, someone standing behind the person hands him something ridiculous . . . like a watermelon or a loaf of French bread. And everyone they do it to is BAFFLED by it.
In fact, a few of them actually believe that the thing he pulls out WAS in their hair.
Kane is joined by co-hosts Riley, Rose, Intern John and producer Erick. The high-energy, fast-paced program features music, conversations on the latest news and hot topics, Hollywood news, contests, call-in interaction with listeners, plus interviews with the biggest celebrities in music and pop culture. The show features signature segments such as "War of the Roses," "Hollywood," "The Tea," "John's Justice," "Ghosted" and "Moron Madness."
Kane Cares! We'd love to hear about your event or organization. Use this link to send us your information!


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Severe stomach pain
My elder brother is suffering from severe stomach pain since 3 days the doctor advised for ultrasound. After ultrasound i came to know that there are some stones in right kidney(size 24*10 mm) and doctor asked for IVP test When IVP test result came when he consulted the doctor he said right kidney is not functioning. I am very worried right now he never smoked and never consume alcohol. since last 6 month he had problem of high BP and swelling in leg below part. I want to ask what were the causes for this kidney problem? what should be done in future ? what is the cost for removing right kidney and what will be the consequences when he will have only one functioning kidney? Please suggest how there should not be condition for removing right kidney.. Please help
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The kidney had probably shut due to the high BP for a long period which has probably damaged both the kidneys. The hypertensive nephropathy is probably the cause for oedema (swelling) in the legs. There are only a few conditions where removal of the kidney is indicated. How old is your brother ? What are his kidney function test results ? Need to know more details.
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Jem: The Second AttackMature
I sat near the fire, whittling another small wooden flute, thinking about the mysteries in my head. I noticed Griever releasing a mail pigeon, and it flew off to the north for a moment, then looped around and headed south. The letter could have contained anything, most places were south of Hamish, but once again, I felt my stomach drop with nervousness and tension.
I blew a few experimental notes on the flute, and was about to stand up as Kate approached the fire.
"Jem. I've just been informed there's a replacement delivery arriving today." She said as she sat down. "I think two deliveries raided in a week would cause a fair bit of trouble for our new Marshall, don't you?"
I smiled grimly. "Perhaps, though it could be dangerous to do two in a week. It could cast suspicion on this part of the forest."
"So we attack from another place." Kate pressed. "Before the cart reaches the edge of Hamish. In the midst of the forest perhaps."
"That could work. If the convoy comes at the same time as it did before, we will have to change the time of the attack." I stood up and handed Kate the flute. "I'll take a team and do some scouting for ambush points this morning."
"Excellent plan. Shall we entrust roles in this to our new recruits?" Kate asked.
I thought about it for a moment. "The man Greiver, perhaps. I do not trust him, but his strength could come in handy when its time to hide the evidence. The girl should stay, its too dangerous for her until she's had some training with a weapon. We should get someone to teach her immediately."
"Why do you not trust him?" Kate asked, her tone was almost accusing. "He was helpful during my trip to the market. It is his knowledge that gave me the name of the new Marshall. The girl, I agree with you on her account. Perhaps Kiara would train her?"
"I dont know." I thought about Griver and the letter he sent earlier. "Something about him sets me on edge. His timing was just too perfect, and his manners are just this side of too refined. What if he was sent here by the sheriff? As for Kiara, she would be a tough teacher, but patient. I think she would be a good choice."
"Or perhaps he is well trained and well brought up. We cannot know until we have tested him as we do all our newcomers. I will tell Kiara to begin immediately after our raid."
"Alright. I will take a team out immediately and prepare for the raid." I walked away, calling out to several members of the night hawks to prepare to set up an ambush.
"Griever, you're coming too," I ordered bluntly. Just because he would be helpful, didnt mean I had to like it.


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- Radeon HD 5870
- PCI Express 2.1 x16
Some manufacturers place restrictions on how details of their products may be communicated.
Good piece of hardware. But!!!! 10/28/2010
This review is from: ASUS ROG MATRIX 5870 P/2DIS/2GD5 Radeon HD 5870 Platinum 2GB 256-bit GDDR5 PCI Express 2.1 x16 HDCP Ready CrossFireX Support Video Card
Plays almost everygame I have on high @ 1920 x 1200 RES with avg 35Fps But that is the same with any 5870 clocked @ 900mhz and that is not why I bought this card. It overclocks with CCC to 935Mhz with no voltage change.
The software side of this card really needs an update or something. BSOD For every little thing I change in the Asus ITracker. Do not update to bios AS11 if you have Win 7 64bit. Bios AS11 requires ver 1.6 ITracker and there is not that ver for win 7 64bit so this is a wast of money. I had better luck with the ref 5870, and you can not change voltage with any other overclocking utility the voltage regulators are not supported by anything other then ITracker.
Other Thoughts:
Truly not worth the $100+ premium.
1 people found this review helpful. Did you? Yes / No Thanks for the valuable feedback you provided!


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Posted June 14, 2011 by 1KnonOCoKhet in ThatsEnuff
JoJo Remixes "Marvin's Room (Can't Do Better)"
I've been a fan of Jo-Jo since "Get Out," when she was just 14 years old.
This girl's voice is amazing but I must say, it is weird to watch her grow up into an adult female artist.
Well, check out her cover of Drake's new track "Marvin's Room."


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Madras Café, A movie of international standards without any doubt with a riveting story, great line up of seasoned actors and some brilliant direction by Shoojit Sircar – who has firmly placed himself in the top echelon of directors with the likes of Anurag Kashyap & Vishal Bhardwaj. In Bollywood the list of directors who make movies that are riveting, and yet don't insult one's common sense, are few and far in-between – Shoojit Sircar is certainly amongst them .
The film is about the plot to assassinate Rajiv Gandhi and the events leading up to it. It is about the botched up attempt by the Indian government to bring peace in Sri Lanka, the ego and arrogance of Prabhakaran – leader of the LTTE. We know now in hindsight that the LTTE was a big mistake – The Tamils in SriLanka are far worse off now than before. The movie shows how the Indian government tried to create alternatives for the Tamils in Sri Lanka – again in hindsight this appears to have been the correct move which unfortunately failed. Now after two decades the quest for Eelam (a distinct and different nation) or that of autonomy (provincial governments) has been reduced to calls for humanitarian help. The assassination of Rajiv Gandhi closed many doors in India (to the LTTE) and firmly placed the LTTE as a terrorist organization within the world community leading to its ultimate demise.
The only weak section, I feel, in the film is the sketchy details of the so-called foreign conspiracy. The foreign element, including the involvement of elements from the United States and Singapore, was vague at best. But I wonder if this is a flaw or a planned attempt at a future sequel ? Which I hope there will be. John Abraham plays the leading role well, Nargis Fakhri plays the "femme fatal" part well – there was no real need for her role in the film; however, the director managed to weave in her character in the film with ease thereby getting a pretty face in the storyline.
There is an important part of the movie that should be highlighted vis-à-vis the plot to assassinate Rajiv Gandhi – The soldiers and our intelligence staff were doing their job with diligence, at times with their lives at risk. But when it came to the final decision making to ensure the safety of the then ex-prime minister –a cabinet secretary (in the movie) said – "we will just give this information (about the probable assassination) to the state police and let them handle it". You are clearly only as strong as your weakest link and the weakest link in our country seems to be at the very top. Politicians and bureaucrats who know how to come to power , get promoted with various means , but have no clue how to manage operations or have any foresight when it comes to economic development – their capability is their ability to get to and retain power and nothing more usually. The proof is in the pudding (there are countless examples of this) – else how does a small guerrilla army assassinate an ex-Prime Minister of a country having the fourth largest army in the world? Also an interesting point comes to light – Rajiv Gandhi had actually resigned his post as PM in response to the dismal failing of the Indian army to bring peace in the Jaffna region – clearly a man of higher morals than the current bunch. Maybe those currently in power should take a cue, resign and go back to the people. That will not be possible today as Corruption (Government) is like a business, there is a pay-off every month and eight months (to the next election) is quite a few months of pay-offs.
Madras Café also highlights the economic importance of the Trincomalee port in Sri Lanka and the interests of foreign elements in controlling that port. India has a long and economically important coast line. However the travel from the eastern part of India to the western part (or vice-versa) has a hump – that of Sri Lanka. The Sethu Samudran project would save an approximate 30 hours of travel time and 420+ nautical miles of travel and reducing the importance of any of the ports in Sri Lanka. Of course our bureaucrats have had their way and the entire project has essentially been scuttled – a foreign conspirator was not required – our bureaucracy did the job!
While the movie claims that it may not be based on actual facts (as any other movie) – it is a well-research and well-executed. A "must-see" film !
Also See:
Madras Café Movie – Prerelease Review
Tamil Separatism and the Demise of the LTTE: An Analysis
Image Source: SethuSamudran


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NCHMCT JEE Counselling
Are you ready for NCHMCT JEE Counselling? If yes then check out NCHM Round 1-2-3 here! National Council for Hotel Management & Catering Technology is all set to conduct Counselling of all the candidates for the Allotment of seats. Aspirants who appeared in Joint Entrance Examination for getting admission in various courses such as B. Sc in Hospitality & Hotel Administration, Diploma in Housekeeping, Diploma in Food & Beverage Service, Diploma in Bakery & Confectionery and others, they have to now appear in NCHMCT JEE Counselling 2017.
Aspirants can check National Council for Hotel Management & Catering Technology Joint Entrance Examination Schedule from this page. We the team of www.recruitmentinboxx.com has displayed all the details about NCHMCT JEE Counselling in this page. You can book mark our page by pressing Ctrl + D to collect all the upcoming details.
NCHMCT JEE Counselling
Procedure of NCHMCT JEE Counselling:
NCHM Counselling is commonly based on 3 rounds which are described below:
- First of all, applicants, who are going to attend counseling, have to register Online. For registration, you have to log on to the web portal of organization and after that, search tab of "Online Registration and Choice Filling".
- Applicants, have to fill required details such as Roll number, Application number, Candidate's Name and Date of Birth.
- Applicants need to choose password and security question and their answer.
- After completion of all process, your personal data will be appeared on the screen. Applicants need to verify their personal data.
Check out Requirement of Documents for Counselling: Press Here
1. Choice filling and locking for 1st round:
- In this section, Applicants, who get registered, need to apply their choices of institutes according to their interest.
- Applicants can apply according to their choices through appropriate messages and tabs which is display on screen.
- After that, appliers have to fill their choices in the order of preference from the given list of available institutes.
- Participants are suggested to lock their choices before the last date.
2. Seat Allotment for 1st Round:
- After completion of above process, the further processes will procedure centrally as per the Seat Allotment Algorithm.
- Applicants will acquire seats allotted in the order of merit as per AIR and also on the basis of applicable reservation criteria.
- After that, result will be declared on web portal of organization and you need to login with Roll No and date of birth to check the performance card.
- Applicants have to deposit an amount of Seat Acceptance Fee and required to report personally to any Reporting Centers.
To Know List of Best Engineering Colleges In India: Visit This Link
2nd Round of Counselling Process:
Candidates, who have already registered in 1st round, they may appear in the 2nd round of counselling.
Eligibility for 2nd round:
|Registered candidates who are not allotted any seat in the first round of seat allotment. These candidates are permitted to change their earlier submitted choices through internet.
|Candidates who have been allotted a seat in the first round of counseling and on reporting their allotted seat has been cancelled due to non fulfillment of categories criteria
|Candidates who have been allotted a seat in the first round of counseling and reported at the reporting centre and also submitted willingness for up gradation in affirmity
|Candidates who have been allotted a seat in the first round of counseling and reported at the reporting centre and also submitted willingness for up gradation in negative, these candidates would not be allowed to submit fresh choices and their earlier allotted seat would be retained
You Can Get All Counselling Schedule : From Here
3rd Round of Counselling
3rd Round of counselling will be organize for those students, who cannot attend 1st and 2nd round of counseling and also all other students, those had appeared in exam.
Important Dates for NCHMCT JEE Counselling (Tentative):
|Important Dates
|Online Registration & Choice filling for 1st & 2nd round of Counselling
|May/June 2017
|Choice Locking
|June 2017
|1st Round of seat allotment
|Reporting at Reporting Centre after 1st round of allotment for document verification
|Vacancy due to non-reporting/Withdrawal/ Cancellation
|Choice modification for 2nd round of counselling (Optional)
|2nd Round of seat allotment
|Reporting at Reporting Centre after 2nd round of seat allotment (only Fresh allottee of this round)
|Tentative Vacancy due to non-reporting/Withdrawal/ Cancellation
|SPOT (3rd) round of Counselling, Registration, Fee deposit, Choice filling & Locking(web based)
|SPOT round seat allotment
|July 2017
|Reporting at Reporting Centre for document verification
|Physical reporting at allotted Institutes by all candidates who have allotted a seat in any rounds and submission of Hostel preferences
|Hostel Allotment Centrally and result will be published on website (Admission closed)
|Commencement of Classes
For further details about NCHMCT JEE Counselling 2017, you can check our page that is www.recruitmentinboxx.com again. If you have any query related to the posts, you can simply leave a comment in below mention comment box. Our experts will try to sort out your problem as soon as possible.
You May Also Like To Visit This Section
|NCHMCT JEE Application Form
|NCHMCT JEE Admit Card
|NCHMCT JEE Result
|Entrance Exams In India
|Scholarship in India
|How To Apply For Education Loan
|How To Survive First Year Of College
|Why College Attendance is Important


text: Tech Choose Your Password Carefully December 14, 2010December 30, 2010 Mike Johnson People that post on sites like Gawker, Kotaku and DeadSpin are web savvy, but they're pretty simple when it comes to their passwords. Chart below shows why the US won't be a super power in about 10 years. (via WSJ)


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Kidango is hiring! Are you ready to influence the lives of young children? Kidango is looking for caring individuals with dynamic personalities to educate young minds. We offer a generous benefit package to complement our active job opportunities.
We are actively recruiting for these positions:
To apply for one of these positions, please complete the following three steps:Online Employment Application
Online Applicant's Statement
Online Equal Opportunity Employment Form
Why Kidango?
Kidango offers an innovative benefit package for their employees. Benefits include reduced tax sheltered accounts, dental and medical coverage and more! Kidango is proud of its diversified workforce. We pledge equal access to services and employment regardless of race, color, religion, gender, age, national origin, disability, or sexual orientation. Try Kidango and you'll see why our employees are smiling.
Email HR for any further questions: firstname.lastname@example.com


text:
Service Description
The Web Services & Technologies Group creates web applications for a variety of college and department needs. Specifically, when users need to gather information and display information, both secure and non-secure, to one another and the world. Broadly, the service categories include but are not limited to forms, web pages and web apps. All systems and solutions support the academic mission, administrative and business functions of the University.
Included Services
- Application development
- Updates/changes to existing applications
Service Examples
- Dining Hall Menu
- Events Calendar
- Event Registration
- MyPhoto
- NewsWire


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Two The Amazing Spider-Man Posters
Columbia Pictures has revealed two new posters and a new photo for Marc Webb's The Amazing Spider-Man, starring Andrew Garfield as the webbed wonder. Take a look at a mid-air shot of Spidey in the photo, and the superhero's reflection in the Lizard's eye in the 1-sheets.
Don't Miss A Story


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Keep the Information Flowing
Small contributions go a long way. Your donation to Consumer Action, a 501 (c)(3) nonprofit, nonpartisan organization, can help us cover the cost of research, writing, and translation of our materials. To keep our services free for those who need them. Select an amount to give.
Link Last Checked: August 17, 2015
Consumer Resources Links
A non-profit network of 17 public interest justice centers in the United States and Mexico, Appleseed is dedicated to building a society in which opportunities are genuine, access to the law is universal and equal, and government advances the public interest.
Visit: Appleseed
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Support Consumer Action
Join Our Email List
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- Help Desk
- Submit Your Complaints
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text:
Welcome to dickblick
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Anticipating how kids react to paintings by artists as diverse as Botticelli and Pollock, this prepares adults for the questions kids ask. He offers valuable tips for making any museum visit a success by providing a great refresher course on art history.
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© Copyright 1999-2017 Dick Blick Holdings Inc. All rights reserved.


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Manage Reservations
Need to make changes to your reservation? You've come to the right place!
Already an IHG® Rewards Club Member? Sign in to view reservations.
Restaurants On-site: 1
Off-site Restaurant Delivery Available
Room Service Available in Executive Rooms, Suites and Rooms from
Mare, the fourth restaurant in Alessandro Pirozzi's Culinary Collection, is intimate and chic and is the perfect place to pop in for martinis and scrumptious appetizers. The hip, internationally inspired decor that's comfortable for every age and the imaginative cuisine brings haute dining to a piquant new culinary level. New Winter Hours : Breakfast: 7:30 a.m to 10 a.m Dinner:4:00 p.m. to 10 p.m
Location: Street Level
Serves: Breakfast, Brunch, Lunch, Dinner, Dessert


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Item: SW282
A friendly bacteria
Each capsule contains more than one billion viable organisms
Aids digestion and helps maintain a healthy balance of microflora
2 Billion CFU 100 Caps
Caps Size: 0
Featured review
"I have recommended it to several friends who are now ordering and using it regularly. They all say they love it!" ~product review by TenthinsightSee all reviews
Serving Size 2 Capsules
Servings Per Container 50
|Amount Per Serving
|% Daily Value
|Lactobacillus acidophilus
|2 billion viable organisms†
*Daily Value not established.
†At time of manufacture.
Other ingredients: Gelatin, may contain one or more of the following: microcrystalline cellulose (plant fiber), magnesium stearate, silica.
Suggested Use: As a dietary supplement, take two capsules with water before a meal. For children, one capsule with water before a meal.
.16 lbs
A friendly bacteria
Each capsule contains more than one billion viable organisms
Aids digestion and helps maintain a healthy balance of microflora
Aid digestion and maintain healthy flora with Swanson Acidophilus! While we normally think of bacteria as bad for us, there are certain "friendly" species, such as Lactobacillus acidophilus, that populate the intestinal tract, where they aid in digestion and help maintain a healthy balance of microflora. A great way to replenish your body's levels of these health-promoting allies, each capsule deliver over one billion viable organisms at time of manufacture.
"With my personal 100% money-back satisfaction guarantee, you have nothing to lose and great savings to gain."
Lee Swanson, Founder of Swanson Health Products
If you are dissatisfied for any reason, we'll give you a full refund and pay return shipping.
We are dedicated to offering the highest quality products at the best price. Find out more about how we ensure such a high standard in everything we do.Learn More
2 Billion CFU 100 Caps
Caps Size: 0
2 Billion CFU 250 Caps
Caps Size: 0


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Systems Biology Opens New Research Pathways
Systems Biologists Are Attacking the Encrypted Messages That Would Allow Us to Predict and Change the Course of Disease
Individual Tumor Profiling
Has Tumor Molecular Profiling Enabled More Effective and Less Toxic Cancer Treatment?
Top 10 Under 40
Up-and-Coming Stars Shine in Biopharma Research and Business
Podcast: FDA's New Commissioner Hits the Ground Running
Scott Gottlieb, M.D., Commits to Speeding Up Drug Reviews, and Maintaining Safety and Efficacy
Automated HT Reverse Transfection of Plasmid DNA
Results from Studies with Two New Reagents Showed Compatibility with Accepted Methods
Click Image To Enlarge +Figure 1. Formation of the transfection complex, cell seeding, and transfection were performed in a 96-well format using an automated workstation.
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection. In this study, X-tremeGENE HP and X-tremeGENE 9 Transfection Reagents (Roche) were tested for their compatibility with an automated transfection workstation, the Biomek FX (Beckman Coulter).
Material and Methods
Automated Transfection
Cell seeding and transfection were performed using the Biomek FX automated workstation. For the transfection, X-tremeGENE HP and X-tremeGENE 9 reagents (Roche) were prediluted in Opti-MEM (dilution factor: 1:50 for X-tremeGENE HP reagent, 1:33 for X-tremeGENE 9 reagent). The EGFP-PC3.1 plasmid was prediluted in Opti-MEM (1 µg/100 µL). The transfection-complex was formed by pipetting the same amount of plasmid dilution into the dilution of transfection reagent, followed by mixing and incubation for 30 min.
Cell Culture and Reverse Transfection
HeLa cells (ATCC® CCL-2™) were cultured in MEM with supplements at +37°C in a 5% CO2 humidified atmosphere in T75 flasks until a confluence of about 70% was reached (Figure 1). Cells were harvested by trypsinization shortly before transfection, washed and resuspended in fresh medium (1.2x105 cells/mL). Reverse transfection was performed by pipetting 100 µL cell suspension to each well already containing the transfection complex.
- Formation of the transfection complex, cell seeding, and transfection were successfully performed in 96-well format using an automated workstation. Increasing volumes of transfection complex from 0 to 20 µL were added to each well in replicates of s16 for each volume.
- The efficiency of transfection was found to be directly dependent on the added volume of transfection complex. As expected, higher transfection efficiency was measured at higher complex volumes unless a plateau phase was reached (Figure 2).
- Both transfection reagents showed very low cytotoxicity for all volumes tested (not shown).
- X-tremeGENE HP and 9 Transfection Reagents are fully compatible with an automated transfection method and show a high rate of reproducibility.
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