text:
Amarath Roth of A Shadow's Tale
1 - What is your name?
Amarath. What? You need more than that? Fine. Amarath Lycorn Roth, ok?
2 - How old are you?
I don't know. I gave up counting. Somewhere in my twenties, I think.
3 - Where do you come from?
Over the hills and far away.
4 - Are you married/in love?
Let's see... First guy got me pregnant and left, second guy turned out to be a demon hunter and the third guy died a very early death. I think it's enough to say, no, and never again.
5 - Do you have any children?
Yeah, two girls, Draconiss and Keegal.
6 - What is your profession?
I'm the weapons master of the clan, and you're going to be on the wrong side of my expertise if you don't stop annoying me!
7 - Do you have any secrets?
No! Well... Ok, so I still sleep with a teddy bear. I'M NOT THE ONLY ONE!
8 - Who is your best friend/worst enemy?
Best friend? I'd have to say Shadow. Sad, isn't it? My only best friend is my sister. As for worst enemy, at the moment? You. In general? Pretty much everyone.
9 - What would you take out of a burning house?
Archangel. The stupid prankster probably started it.
10 - Name three weaknesses and three strengths.
Ok... I have a limp, I have a temper and my sister says I have a really nasty sense of humour. I don't believe her though.
Strengths? I'm good with weapons, bad with kids, (yes, that's a strength. It means I don't get landed with babysitting duty), and I'm a good tactician.
11 - If you could change 1 thing in your past, what would it be?
I'd go back and save my sister.
12 - What is your first thought in the morning?
How to disable the pranks Archangel probably set up during the night.
13 - What are you terrified of?
I'm not scared of anything!
Apart from clowns.
Clowns scare me.
14 - What is the 1st thing on your Christmas list?
Gunpower. I probably won't get it though. My family's scared enough of my arsenal as it is.
15 - What is the worst present anyone's ever given you?
A fake, squeaky sword. Thank you, Archangel...
16 - Who would you want to talk to one last time? What would you say?
I'd talk to Shadow, to tell her I'm sorry I wasn't fast enough.
17 - Would you consider yourself an angel or a devil?
A devil. There's absolutely no way you could mistake me for an angel.
18 - What would your 3 wishes be?
Pfft... I don't wish for anything. No point in wishing.
19 - What makes you angry?
Snarky little gits -glares-
20 - Would you like a hug?


text:
You should've come over
Rain in these skies
are like sheets of smoke
they growl only silently
behind, melancholic mockery
Like your cold hugs
they rush past my silhouette
and, scratch patterns on my walls
through wine bottles on the windowsill
This air smells like newspapers
under the old coffee table,
and pennies from my aunt's purse
they smell rhubarb jelly Christmas cake.
But the pallor of these rheumatic mornings
envelop my plume of thoughts
no, slumped at my feet, bed- ragged
as a grassland in oblivian.
If oceans were made of tears
and skies of joy,
then this must be joyful tears.
But they're not...
and these are the grasslands they nourished.
These dew drops must belong to you
and the mist
and the ardour
and the fake Christmas smiles too.
It's no surprise that this is the harvest we reap.
Because after all,
you never came.

subset: commoncrawl


text:
Celtic Kings
Sometimes, when we feel a little Irish, we'll go over to the pub, drink some Tullamore Dew, imitate Thomas Mitchell's thick Irish brogue in Gone With the Wind and talk about how precious land is to an Irishman. Other times, we'll just put on a Chieftains album and start dancing. If we're feeling particularly Irish, we might just do both. The Chieftains are coming to town, and if you've never heard of them, get yourself to the Winspear Opera House and buy a ticket. The band, founded in 1962, plays traditional Irish music on traditional Irish instruments. But don't let the description fool you, because the Chieftains are one of the most innovative and longest lasting bands out there. Maybe it's that Irish whiskey courage, but these guys have never been afraid to stray out of their comfort zone, delving into the worlds of rock, punk, country and even mariachi music. No matter what they're doing, their music is the kind that sets your toes to tapping. The Chieftains take the stage 8 p.m. Saturday at the Winspear Opera House, 2403 Flora St. Tickets are $19 to $100. Call 214-954-9925 or visit attpac.org.
Sat., Feb. 26, 8 p.m., 2011
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text:
हेर्नुहोस भिडियो
Another trick video has been transferred on YouTube and it can be an exceptionally entertaining trick video. The trick video is called 'Don't Look Within Girlfriends A$$ Prank' and the trick video has an expected 2 million perspectives.
In the trick video a couple go out and in addition the sweetheart is exceptionally breathtaking and she is additionally wearing tights to mitigate they. As men stroll around and take a gander at her, her beau comes without hesitation and begins to contend with the men concerning why they were looking at his sweetheart's general body. The video is exceptionally clever as it practically transforms into af!ght numerous period.
Lion's share of the men have comparable response and has as of now been impressively gets significantly more entertaining when the sweetheart says that they is drained alongside her beau and beeps with the other arbitrary man.


text:
3830 Dupont Avenue N, Minneapolis, MN 55412
About the Listing
Tastefully remodeled and ready for your family. Updated kitchen and baths all boast new tile work. New SS appliances. New carpet and gleaming hardwood as well as fresh paint make this home truly move in ready. Unique 2 story with a spacious master bedroom on upper level with skylight. Basement is clean and dry with a new washer and dryer. Flat, family friendly fenced yard. City of Mpls Certificate of Approval has been issued. Take a look at this home. You won't be disappointed.
Share Listing
Property Details for 3830 Dupont Avenue N, Minneapolis, MN 55412
Bedrooms: 4
Bathrooms: 1 Full | 1 Three-Qtr
Lot Size: 0.11 Acres
Lot Dimensions: 40x126
Year Built: 1921
Garage Dimensions: 1 car
Garage: Detached Garage
Style: (SF) Two Stories
Neighborhood: Webber - Camden
Water: City Water/Connected
Sewer: City Sewer/Connected
Square Feet: 1598
Above Ground Sq Ft: 1598
Room Information for 3830 Dupont Avenue N, Minneapolis, MN 55412
Main Floor
Living Room: 15x12
Dining Room: 12x10
Kitchen: 16x9
Bedroom 1: 10x10
Bedroom 2: 10x10
Upper Floor
Bedroom 3: 26x17
Bedroom 4: 11x12
Dining Room Description: Living/Dining Room
School Information for 3830 Dupont Avenue N, Minneapolis, MN 55412
School District: Minneapolis
School District Phone: 612-668-0000
Interior Features for 3830 Dupont Avenue N, Minneapolis, MN 55412
Appliances: Range, Exhaust Fan/Hood, Refrigerator, Washer, Dryer
Air Cond: None
Heating: Forced Air
Fireplaces: No
Basement: Full, Day/Lookout Windows
Exterior Features for 3830 Dupont Avenue N, Minneapolis, MN 55412
Lot Description: Tree Coverage - Light, City Bus (w/in 6 blks)
Lot Dimensions: 40x126
Roof: Asphalt Shingles, Age 8 Years or Less
Zoning: Residential-Single
Financial Considerations for 3830 Dupont Avenue N, Minneapolis, MN 55412
Tax Amount: $1,582
Taxes w/Assessments: $1,582
Tax/Property ID: 0402924410225
Tax Year: 2016
Monthly Payment = $0
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The data relating to real estate for sale on this web site comes in part from the Broker Reciprocity Program of the Regional Multiple Listing Service of Minnesota, Inc. Real estate listings held by brokerage firms other than the owner of this website are marked with the Broker Reciprocity logo or the Broker Reciprocity thumbnail logo and detailed information about them includes the name of the listing brokers.


text:
Ganesha gold plated yantra
Categories: Yantra- Task
Ganesha gold plated Yantra
Beneficial for success task/work, wealth.
This Siddha Ganesha gold plated Yantra charged by Panchmukhi Ganesha mantra. Ganesha gold plated Yantra removes Hurdles and ease New beginnings .One who does the upasana & Puja of the Ganesha Yantra is blessed with success in his work, business, enterprise & desires. Ganesha Puja is should earlier than beginning any work. It's most auspicious. The sadhaka is blessed with success in his work, business, enterprise and desires. Ganesha Puja could be carried out through idol or by way of a Ganesha Yantra. Ganapati or Ganesha, the Lord of Ganas, the elephant faced God, represents the facility of the Supreme Being that removes obstacles and ensures success in human endeavours. Ganesha is revered as the son of the Shiva and Parvati, and is all the time honoured first in most worship services and rituals. He's also the God of education, data and wisdom, literature, and the high-quality arts. Ganesha Yantra is embossed on a sq. plate of pure copper and has been specially energised to radiate its beneficial powers. Ganesha Yantra might be positioned in your temple at home, in your work table or in your cash box.
You can keep this Siddha Ganesha gold plated Yantra in the office or home (worship place) or Two Ganesha gold plated Yantra can buy for office and home.
Ask Question about this "Ganesha gold plated yantra"


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Game Doesn't Progress to Main Menu[ Edited ]
I just got this game and all it's done is install patch 1.05 and then sit on a black screen flashing an animated "the last of us" logo in the bottom right corner for literally hours now.
I've tried letting it sit, I've tried resetting the console numerous times, i've deleted all The Last of Us data and let it re-installed the patch - but nothing.
For all intents and purposes it appears that Naughty Dog has sold me a disc that includes nothing but this flashing logo on it instead of the game that I paid them for.
How do I access the game that I just dropped all this cash on, that all of you people swear is supposedly an incredible game? So far it looks like Naughty Dog just scammed me.
Re: Game Doesn't Progress to Main Menu
After many hours I just checked the loading screen again and it's actually been flashing that stupid logo for so long that the console was warning me it was about to go into auto-shutdown due to inactivity.
Thanks a lot for stealing my money and selling me this fake game, Naughty Dog. I used to like you and talk up your games, but now I realize you're nothing but scam artists and I'm sure as hell not bothering with a PS4 or any future titles at this point. I just want my money back - the game is clearly a joke perpetuated by the internet as all that is on the disc is a black screen with blinking logo.
Re: Game Doesn't Progress to Main Menu
Re: Game Doesn't Progress to Main Menu
Do you have any other " Dual layer " disc games? Final fantasy 13, Uncharted 3, Gran Turismo 6? It's most likley due to your PS3 not reading dual layer disc's anymore.
I was in the same boat as you. My 2 year old 120 gig slim no longer reads dual layer disc's so i borrowed my sisters 320 gig slim ( she only uses it for netflix ) And i was able to play through TLOU no problem not 1 freeze or glitch.
Re: Game Doesn't Progress to Main Menu
All of those games work perfectly. The only game that is not functional is this piece of **bleep** they pretended was a game. Evidently it's merely a screen saver.
Re: Game Doesn't Progress to Main Menu
FIX THIS **bleep** GAME YOU DISGUSTING CON ARTISTS.
Re: Game Doesn't Progress to Main Menu[ Edited ]
Hey Jimmy_Chopblock, thanks for trying to delete the TLOU related data and testing on a seperate disc. I have a few troubleshooting things we could try. Before starting, could you please confirm which model of the PS3 you own, whether or not it is an original HDD, and which disc region you have (the spine of the cover lists a code 'XXXX-#####' (e.g. a US disc should read BCUS98174).
Also before we start, it will be important to back up any important data (save data, pictures, videos, etc.) that you might have on the HDD.


text:
The hotel features a free wi-fi internet connection available in the common areas and an innovative automatic catering service, allowing guests to purchase hot and cold food and drinks 24h. In addition the hotel offers a snack bar, a free outdoor parking, a garage connected to the main building, and a 5 room conference center equipped with the most up to date technology. Free Wi-Fi is available in the whole hotel building including rooms.
This new hotel is located in a quiet suburban area close to the main motorway junctions. The establishment is just 1 km from the motorway exit Bologna San Lazzaro, making it an excellent base for those wishing to visit the tourist sights of Bologna, which are 7 km away in the city centre, and also placing business travellers just 6 km from the Bologna trade fair. The hotel is also just 14 km from the airport.
The 209 rooms offer an interpretation of hospitality that is synonymous with refinement and practicality. Spacious, with elegant furnishings enhanced by light woods contrasting with dark Weng� wood, they boast a full range of comforts: air conditioning, satellite and pay TV with charge, direct telephone with charge. Free Wi Fi
The hotel offers an indoor swimming pool with relaxation area.
The reception desk is open 24 hours a day. A laundry service is available 5 days a week. Room service is available from 8 am to midnight.
Room Facilities:
Direct dial telephone
Internet access
Individual heating
Wheelchair accessible
Disabled bathroom


text:
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Check Out the new Remax App
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Estate sale, priced to move quickly!! All brick 5 bedroom on 3.79 acres under $50 a square foot! Needs hvac unit. Great house for a large family. Just before Lake Talavana. Separate kitchen, family room, living room, dining room. Perfect home for a handy man's touch. Outside porch, 2 car carport, over 3400 s.f. under roof.


text:
Current perspectives of the signaling pathways directing neural crest induction
- First Online:
- Received:
- Revised:
- Accepted:
DOI: 10.1007/s00018-012-0991-8
- Cite this article as:
- Stuhlmiller, T.J. & García-Castro, M.I. Cell. Mol. Life Sci. (2012) 69: 3715. doi:10.1007/s00018-012-0991-8
- Citations
- 2.9k Downloads
The neural crest is a migratory population of embryonic cells with a tremendous potential to differentiate and contribute to nearly every organ system in the adult body. Over the past two decades, an incredible amount of research has given us a reasonable understanding of how these cells are generated. Neural crest induction involves the combinatorial input of multiple signaling pathways and transcription factors, and is thought to occur in two phases from gastrulation to neurulation. In the first phase, FGF and Wnt signaling induce NC progenitors at the border of the neural plate, activating the expression of members of the Msx, Pax, and Zic families, among others. In the second phase, BMP, Wnt, and Notch signaling maintain these progenitors and bring about the expression of definitive NC markers including Snail2, FoxD3, and Sox9/10. In recent years, additional signaling molecules and modulators of these pathways have been uncovered, creating an increasingly complex regulatory network. In this work, we provide a comprehensive review of the major signaling pathways that participate in neural crest induction, with a focus on recent developments and current perspectives. We provide a simplified model of early neural crest development and stress similarities and differences between four major model organisms: Xenopus, chick, zebrafish, and mouse.
KeywordsNeural plate border FGF BMP Wnt Notch MAPK Smad β-Catenin
Neural crest
Neural plate border
Neural plate
Non-neural ectoderm
- pNC
Prospective neural crest
- pNP
Prospective neural plate
Dorsolateral marginal zone
Embryonic stem
- EpiSC
Epiblast stem cell
- hESCs
Human embryonic stem cells
NC development is perceived as a step-wise progression from inductive events to transcription factor expression to modulation of migration and differentiation (Fig. 1). The molecules and their interactions have been integrated into a NC gene regulatory network, providing a rich framework for continued functional and comparative research [1, 2]. Initially, the NC is induced by a combination of signals, most notably the bone morphogenetic protein (BMP), fibroblast growth factor (FGF), and Wnt signaling pathways, but also potentially including Notch/Delta, retinoic acid (RA), Hedgehog, and Endothelin signaling. These signaling pathways integrate early in development to induce the expression of a set of regulatory transcription factors (Msx1/2, Pax3/7, Zic1, Dlx3/5, Hairy2, Id3, Ap2), which specify the neural plate border (NPB). These factors along with combinations of the same signaling pathways then trigger the expression of NC specifiers, a second set of transcription factors including Snail2, FoxD3, Sox9/10, Twist, cMyc, and Ap2. NC specifiers are proposed to ultimately control neural crest behavior, from EMT and delamination to migration and differentiation. Because these transcription factors are expressed in other tissues at these and other times in development, their spatiotemporal and combinatorial expression must be considered when associating them with NC development.
Although NC development has been studied in several species, our knowledge of the earliest inductive signaling comes primarily from Xenopus and chick research. Recent evidence from these organisms suggests that the NC is induced during gastrulation, and its early development can be explained in a two-step process. An initial phase of FGF and Wnt signaling during gastrulation induces the NC in the prospective NPB, and a second phase of Wnt and BMP signaling during neurulation maintains the NC population. Although the signaling pathways implicated in NC development appear to be conserved among different species, the source, timing, and precise regulation show considerable variation.
The study of early NC development has attracted significant interest owing to the unique properties of these cells. As a great model for induction, pluripotency, cell-fate restrictions, migration and differentiation, NC development involves most elements of developmental biology. Additionally, defects in various aspects of NC development cause a number of debilitating human health conditions, collectively known as neurocristopathies, including aggressive tumors such as melanomas and neuroblastomas, rare syndromes like Hirschsprung and Waardenburg syndromes, and various developmental malformations such as cleft lip/palate and aganglionic megacolon. Therefore, NC biology is of clinical relevance as well, and a fuller understanding of the signaling mechanisms and tissue interactions giving rise to the NC is critical to develop better diagnostic and therapeutic tools for these disorders.
Timing and transcription factors
The initial expression of NC specifiers also varies across species. In Xenopus, most NC specifiers (Snail2, FoxD3, Sox8/9, others) are first expressed at stage 12, very shortly after the appearance of the NPB, and before gastrulation has even completed [29]. In the chick, however, Snail2 is first evident at stage 6 and not strongly expressed until stage 8 (4-somite stage), several hours after the NPB has formed. Furthermore, definitive NC cells expressing a full complement of NC markers are not apparent until just before migration at stage 9/10 [16]. Despite these differences, the avian NC appears to be specified before gastrulation (having already received the necessary signals for differentiation when cultured in non-inducing conditions) [30], while Xenopus neural folds isolated even at the neurula stage do not maintain expression of NC markers without additional signals [31, 32]. In zebrafish, FoxD3 is expressed first and along with Ap2a has a unique role early in gastrulation [21], while Snail2, Sox9/10, and other NC specifiers label the NC towards the end of gastrulation [33]. In the mouse, NC specifiers such as Sox9/10 [34, 35] and FoxD3 [36] label the neural folds very soon after the expression of NPB markers and immediately before cranial NC migration, which is well underway by E8. Additionally, Snail1/2 seem to have switched expression domains in the mouse, but even more intriguing, a double knockout model eliminating both Snail proteins from the epiblast still generates normal, migrating NC cells [37, 38, 39]. Snail proteins are thought to be crucial regulators of both NC fate and EMT, and thus, other transcription factors must be providing these essential functions in the double knockout. One could envision redundancy or compensatory mechanisms, and Twist, Zeb1, and Zeb2 have been considered as candidates to provide the lost function. While possible, evidence for significant overlap between the direct targets of Snail proteins and their putative substitutes is currently lacking. Perhaps then, the NC gene regulatory network in the mouse has diverged evolutionarily, dispensing a critical role for Snail genes in murine neural crest development. On this note, knockdown experiments in other organisms generally cause a significant reduction of the NC markers tested, but a demonstration of a complete loss of NC would require a more extensive study, and this should be considered when making comparative analyses between NC gene regulatory networks.
Evidence for the inductive tissues
NC formation is thought to occur by a classic induction mechanism whereby a tissue or tissues serve as source of inductive signals that are received by another tissue, resulting in the formation of a unique cell type. Pioneering work on NC induction implicated the mesoderm as a potential source of inductive signals—using salamanders, Raven and Kloos were able to generate NC derivatives by grafting presumptive paraxial and lateral plate mesoderm into the naive ectoderm of the ventral blastocoel [40]. Decades later, the inductive capacity of the mesoderm was confirmed in another amphibian, Xenopus laevis. Snail2 was induced in the naive ectoderm of the blastocoel roof following grafts of the organizer or dorsal or lateral mesoderm [41], or by co-culturing explants of naive ectoderm and the paraxial mesoderm [42, 43]. The paraxial mesoderm was subsequently shown to be required for NC formation, as its removal inhibits Snail2 expression [42, 44].
Other experiments instead suggested that the interaction of neural and non-neural ectoderm led to NC induction. Using two different species of amphibia, Rollhauser-ter Horst demonstrated that neural and epidermal tissues can generate NC cells when juxtaposed [45]. Using pigmented and non-pigmented axolotl embryos in donor/host combinations, Moury and Jacobson later demonstrated that NC cells could arise from both tissues [46]. Similar experiments in Xenopus and chick, grafting neural tissue into lateral epidermis, demonstrated both tissues could yield NC cells in these species as well [43, 47]. A recent Xenopus paper suggests the competence of the NNE to give rise to NC diminishes towards the end of gastrulation, while the NP retains its competence until neurulation [48]. Together, these findings supported a model where NC induction results from interactions between the NP, NNE, and underlying mesoderm. Recent evidence now suggests that the precise involvement of these tissues is species-specific.
In Xenopus, most current models propose that the dorsolateral marginal zone (DLMZ) of the gastrula (which underlies the prospective NC) is the source of NC-inducing signals. The DLMZ expresses multiple Wnt and FGF ligands and the BMP antagonist Chordin [31, 41, 49, 50], molecules known to be involved in NC induction. The DLMZ also expresses a number of other Wnt and BMP signaling regulators including Noggin, Cerberus, Frzb1, Dkk1, Sfrp2, and Crescent. A recent study unveils an interesting role for Snail2 in mesoderm formation and implicates this factor in regulating the signals emanating from the DLMZ, making Snail2 crucial for early events in NC development as well [51]. By neurula stages of development, the DLMZ has given rise to the paraxial or intermediate mesoderm, underlying the proper NC. Recombination experiments with the DLMZ and animal caps, or grafts of the paraxial mesoderm into ventral epidermis yield expression of NC markers [31, 32, 50]. Furthermore, explants of the NC at neurula stages do not retain expression of NC markers unless co-cultured with paraxial mesoderm, implicating the mesoderm in the maintenance phase of NC progenitors [31, 32]. A recent study, however, suggests the mesoderm is not necessary for neural or NC induction in Xenopus. Injection of an N-terminally truncated form of Cerberus (CerS) inhibits Nodal signaling and mesoderm formation, but still yields expression of Sox3 (a definitive neural marker in Xenopus) and Snail2 at neurula stages [52]. Although they did not analyze later stages to see if the NC was maintained, this is an intriguing finding. The mesoderm appears to be dispensable for NC induction in zebrafish as well; embryos in which Nodal is inhibited and mutant embryos deficient in Nodal signaling lack mesoderm and mesoderm-derived signaling, but still express all NC markers analyzed [53].
In the avian system, various inductive tissues have been reported. Recombination experiments between nascent neural tissue and paraxial mesoderm from later stages (somites/lateral mesoderm) can yield NC derivatives [47]. Juxtaposition of neural and non-neural ectoderm via grafting approaches in vivo, or via explant co-cultures in vitro also yields NC formation [47, 54]. Yet, NC cells can also be generated from epiblast explants at gastrula stages in the absence of mesodermal and neural markers, and without the addition of exogenous signaling molecules [14, 30, 55]. Thus, while the participation of mesoderm seems to be species-specific, it appears that ectodermal signaling alone may be sufficient to trigger NC induction in the species tested so far. Importantly, there have been no studies regarding the tissue interactions that generate NC in the mouse or any other mammal.
Major signaling pathways involved in neural crest induction
The molecular era of NC induction was launched in 1993 with studies identifying important effects of FGF2 and Notch in Xenopus [56, 57], and Dorsalin1 (a TGF-β molecule) in chick [58]. A series of studies in the following years firmly established the participation of FGF, BMP, and Wnt signaling [41, 42, 43, 44, 59, 60, 61, 62, 63, 64, 65, 66]. Today, much progress has been gained in understanding the molecular underpinnings of these and other molecules during the earliest stages of NC formation across different species. Here, we summarize current perspectives on the participation of BMP, Wnt, FGF, and Notch signaling pathways in NC induction among the major model organisms: Xenopus, chick, zebrafish, and mouse.
Bone morphogenetic protein signaling
Bone Morphogenetic Proteins (BMPs) are members of the TGF-β superfamily of secreted signaling proteins. BMPs bind to Type I and Type II BMP receptors, and in the canonical pathway lead to activation of Smad1/5/8 proteins. Upon ligand binding, Type I and II receptors form hetero-tetramers, with Type I phosphorylating Smad proteins on their C-terminal domains. Activated Smad1/5/8 proteins then form dimers with Smad4 and translocate to the nucleus and initiate changes in gene expression. BMP receptors are also capable of activating signaling through other, non-canonical pathways such as those mediated by TAK1 (a MAP kinase kinase kinase), but only the canonical Smad1/5/8 pathway is currently known to act in NC induction. Throughout development, BMPs have multiple roles in axial patterning, cell-fate decisions, and left–right asymmetry, and misregulation can lead to cancers (for a review, see [67, 68, 69]).
BMP signaling has a crucial role during early development in the establishment of dorsal–ventral polarity and the promotion of epidermal over neural cell fates. The lateral/ventral expression of Bmp ligands and the dorsal/medial expression of BMP antagonists provide the potential to create a gradient of BMP activity. Since the NC forms at the interface between medial and lateral tissues, it was proposed that an intermediate level of BMP signaling is necessary for NC induction. Indeed, epidermal, NC, and neural fates can all be induced in explanted Xenopus ectoderm by increasing levels of Noggin, supporting the gradient hypothesis [44, 70]. A similar BMP gradient model was proposed in zebrafish [71], and recent evidence suggests a BMP gradient may initially specify the prospective NC domain at the late blastula stage [72]. Another interesting study suggests BMP patterns the ectoderm from anterior to posterior progressively during gastrulation [73]. Although the BMP antagonists Chordin, Noggin, and Follistatin secreted from the organizer and dorsal mesoderm are crucial to establishing a gradient, they may not be necessary for NC development in all organisms. In Xenopus, Chordin morpholinos targeted to the DLMZ cause a loss of Snail2 expression in conjugates with animal caps [31], but in zebrafish, morpholino knockdown of all three BMP antagonists still yields a small domain of NC [53]. Interestingly, double-homozygous null mouse mutants for Chordin and Noggin actually present increased expression of NC markers Msx2, Ap2, and Sox10 at early stages. Later NC populations are expanded and undergo precocious migration, suggesting that BMP antagonists in the mouse actually may serve to suppress NC development [74]. Thus, BMP activity might be modulated to levels permissible for NC induction by other mechanisms in addition to or apart from these secreted BMP inhibitors. Other BMP antagonists have been identified, and support already exists for direct regulation of BMP ligands and intracellular regulation of Smad proteins by FGF/MAPK signaling (topics discussed later).
More recently, an alternative explanation to the gradient model has been put forth. During gastrulation, a partial or complete inhibition of BMP signaling is adequate to create a "competency zone" to allow other signals (Wnts, FGFs) to specify the NC [31, 53]. Then, BMP signaling must be activated in the NPB at neurula stages to allow the full complement of NPB and NC markers to be expressed. An intriguing new study using Xenopus and zebrafish embryos has identified a novel nuclear factor, SNW1, which may mediate this shift in BMP signaling, being responsible for a domain of BMP activity in the prospective NPB at late gastrula stages. SNW1 morphants lack a defined NPB and display clear reductions in early NC markers. Targeted overexpression of bmp2b in zebrafish can rescue this phenotype and restore snail2 expression, suggesting the role of SNW1 in NC development is based on its regulation of BMP activity [52].
Recent experiments from chick embryos also suggest that BMP signals act in two phases and argue against a gradient of activity. Treatment of prospective NC explants from gastrula-stage embryos with Noggin for the first 10 h of culture has no effect on their fate, but treatment after 10 h causes a loss of NC markers and an induction of neural markers. Similarly, treatment of prospective NC explants with Bmp4 after the first 10 h of culture causes no change and crest markers arise normally, but if Bmp4 is applied from the beginning of culture, the explants become prospective epidermis [30]. Together, these findings suggest that neural and epidermal cell fates require continued BMP inhibition or BMP activation, respectively, while the NC is generated by an early phase of inhibition and a late phase of activation. These findings are supported in vivo—Smad1/5/8 signaling is essentially absent during gastrulation, but becomes progressively activated throughout the NPB and NNE by neurula stages, with a sharp drop in activity at the NPB/NP boundary [75, 76]. Also, inhibition of Smad signaling causes a loss of NPB markers [76] and experiments on later-stage (stage 10) chick embryos demonstrate BMP signaling is necessary for the expression of NC specifiers as well [65]. Overall, these studies suggest that BMP/Smad signaling can be completely inhibited at gastrula stages to allow NC induction, but must be activated in the NPB upon neurulation to maintain NC progenitors. This activation of BMP/Smad signaling at neurula stages in the NPB may function to promote the NC fate over the neural fate, as the addition of Bmp4 to NP explants from multiple stages can elicit the expression of Pax7, Snail2, and Sox9 [55, 59, 60, 66, 77, 78].
Although the participation of specific BMP ligands has not been directly addressed in the chick, Bmp4 expression closely matches the pattern of Smad1/5/8 activation and is postulated to establish the majority of BMP activity at early stages [75]. Expression of Bmp4 and Bmp7 becomes enriched in the neural folds and adjacent ectoderm at later stages and exogenous Bmp4 protein can induce NC markers in various contexts in both chick and Xenopus tissues [30, 31, 55, 59, 60, 66, 77, 78]. In zebrafish, mutants for both bmp2b (swirl) and bmp7 (snailhouse) lack NC cells, suggesting a shared role in establishing the necessary domain of BMP activity [71, 79]. Notably, bmp2b in zebrafish is proposed to be functionally equivalent to Xenopus Bmp4 [80].
In the mouse, knockouts for Bmp4, Type I BMP receptors Alk2 and Alk3, and Type II Bmpr2 die before or shortly after gastrulation, precluding analysis of NC induction [81, 82, 83, 84, 85]. However, heterozygous mutants for Bmp4 show some craniofacial abnormalities, suggestive of a role in NC development [86]. Bmp2-null mutants can survive until E10.5 and lack migrating streams of cranial NC cells and do not develop the first two branchial arches [87, 88]. A follow-up study demonstrates Bmp2 is necessary for migration, but not for induction, since Bmp2-null mice do express early NC markers Ap2, Snail1, and Id2 [89]. An epiblast-specific (Mox2-Cre driven) knockout for Alk3 (Bmpr1a) has been generated, and presents expanded anterior neural markers at the expense of surface ectoderm and caudal neural markers. NC markers Msx1, Pax3, and Sox10 are still expressed, however, suggesting Alk3 is not necessary for initial NC induction in mouse [90]. Another study uses a Pax3-Cre to conditionally remove Alk3 in the prospective NC and demonstrates that early NC markers are still induced in the cranial region, but NC development in caudal regions is delayed or impaired [91].
A murine line expressing the Cre recombinase from the Wnt1 locus has provided a fruitful tool to analyze later, post-induction events in neural crest development. Wnt1 is first expressed in the dorsal neural tube, specifically in the NC population at the midbrain region at the four-somite stage shortly before NC migration. Given the earlier expression of Msx1/2, Pax3/7, and Ap2, Wnt1-Cre lines cannot address events leading to the initial induction of NC cells, but are valuable for the analysis of later events. A knockdown of Alk2, Alk3, or Alk5 using the Wnt1-Cre causes severe craniofacial, pharyngeal, and cardiac defects, indicating a role for BMP signaling in later NC development [92, 93, 94, 95]. Targeted disruption of Smad4 similarly causes multiple craniofacial, pharyngeal, and cardiac anomalies, partially owing to increased levels of apoptosis [96, 97, 98]. Although the expression of Msx1/2, Ap2a, Pax3, and Sox9 at E8.5 is normal in these embryos, expression from E9.5 on is strongly downregulated, implicating Smad signaling in the maintenance of NC markers [98]. Smad4 also participates in Smad2/3 signaling downstream of other TGF-β family members, however, so these phenotypes result from a loss of all Smad signaling. Indeed, Wnt1-Cre mediated Tgfbr2 knockouts present some of the same defects as those seen in BMP receptor and Smad4 knockouts [99, 100]. Together, these results suggest a later role for BMP/Smad signaling in the mouse, and complement the current models from Xenopus and chick that propose a two-phase BMP requirement for NC induction.
Wnt signaling
Wnts are secreted proteins that initiate a complex cascade of intracellular events, leading to the stabilization of β-catenin in the canonical pathway. Normally, β-catenin is phosphorylated by a complex of proteins including GSK3, APC, and Axin, and then subsequently degraded. Upon binding to cell surface receptors belonging to the Frizzled and LRP families, Wnts cause the activation of Disheveled proteins, which inhibit the β-catenin destruction complex. β-catenin is then free to enter the nucleus where it associates with TCF/LEF family transcription factors to modulate gene expression. Wnt signaling can also activate non-canonical pathways and promote cytoskeletal changes (via Rho-associated kinase in the PCP pathway) or changes in intracellular calcium levels (through activation of PLC and DAG/IP3 signaling). Wnt signaling is implicated in nearly every facet of development and has roles in the generation of multiple organ systems in the embryo. Wnt signaling also maintains a number of adult tissues, and defects in this pathway commonly contribute to cancers (for a review, see [101, 102]).
Wnt signaling has long been associated with NC induction and has recently been proposed to be the inductive signal. In Xenopus, overexpression of several different Wnt ligands can induce ectopic NPB and NC marker expression, and Wnts are capable of inducing NC markers in conjugation and animal cap assays, but only when combined with BMP antagonists (Chordin/Noggin) [3, 4, 11, 49, 62, 63, 64, 103]. Inhibition of Wnt signaling in Xenopus and chick embryos using a variety of extracellular and intracellular modulators has proven the requirement of the canonical β-catenin-mediated pathway for NC induction and later development [3, 4, 5, 7, 9, 10, 14, 31, 49, 63, 64, 66, 78, 104, 105, 106, 107, 108, 109, 110, 111, 112].
Wnt3a and Wnt8 have emerged as strong canonical candidates to induce the NC in Xenopus, being expressed in the DLMZ of the gastrula and at later stages in the caudal NP and paraxial mesoderm, respectively [7, 31, 49]. Specific knockdown of Wnt3a or Wnt8 using morpholinos or a dominant-negative Wnt8 construct inhibits a panel of NPB and NC markers [4, 7, 31, 49]. Interestingly, Wnt3a morphants still express Wnt8, suggesting Wnt3a may act downstream or independently of Wnt8 in NC induction [7]. Wnt8 is also known to be required for zebrafish NC induction. Zebrafish wnt8 is a bicistronic gene, yielding two transcripts (wnt8.1 and wnt8.2), but only morpholinos that interfere with the translation of Wnt8.1 cause a loss of NC markers pax3, foxD3, and sox10 [113].
In the chick, Wnt3a and Wnt8a/c are expressed in the lateral epiblast during blastula stages [114, 115]. At gastrula stages, Wnt3a is found in the epiblast and primitive streak along with a number of other Wnt ligands (Wnt1, 2b, 7b), while Wnt8a/c is expressed in the primitive streak and early mesoderm (S. Chapman, personal communication; http://geisha.arizona.edu/geisha). Addition of Wnt3a to prospective neural epiblast explants can eliminate the expression of neural markers and induce the expression of Msx1, Snail2, and HNK-1 (a marker of migratory NC) [30, 114]. Furthermore, inhibition of Wnt signaling in prospective NC explants causes a loss of NC markers, indicating a requirement for ectodermal Wnt signaling in the chick [30]. Although the potential roles of specific Wnts ligands have not been functionally challenged at early stages, Wnt6 has been implicated in later avian NC development. One study suggests signaling from the NNE activates the canonical pathway in the forming neural folds [66] while another proposes Wnt6 induces the NC through the Rho/JNK non-canonical pathway [116]. Much more work is necessary to determine the source and action of the inductive Wnt molecule(s) in avians, particularly at early stages.
In the mouse, Wnt1 and Wnt3a are expressed just before NC migration and participate in later NC development, but do not play a role in the initial induction. Double-homozygous null mutant mice for Wnt1/Wnt3a initially express Ap2 normally, but expression is lost from the migrating cells. Accordingly, these double-mutant mice have severe abnormalities in NC derivatives [117]. A neural-crest specific (Wnt1-Cre) deletion of β-catenin replicates the midbrain/hindbrain defects of Wnt1 deletion, suggesting Wnt1 signals through the canonical pathway, and additionally presents a near-complete loss of craniofacial structures [118]. Although this β-catenin null mutant makes up for potential canonical Wnt ligand redundancy, the phenotypes may also be the result of compromised cell adhesion. Studies of other Wnt ligands during early murine development do not reveal obvious NC induction defects (summarized, http://www.stanford.edu/group/nusselab/cgi-bin/wnt/mouse).
Additional studies have investigated other components of the Wnt signaling pathway. In Xenopus, the Wnt receptor Frizzled7 has been implicated in mediating the initial Wnt signal in the prospective NC domain, with Frizzled3 likely acting at later stages, perhaps responding to Wnt1 signaling [104, 105]. Lrp6, an LDL-receptor related protein, is thought to be a co-receptor for Wnts in NC induction [109], and participates in signaling with a transmembrane protein, Kremen2 [106]. A novel intracellular PDZ domain-containing protein, Kermit, was also shown to be required for NC development, preferentially mediating Frizzled3 signal transduction [119]. Also in Xenopus, morpholino knockdown of Disheveled 1 or 2 caused an inhibition of Snail2 and Twist, but depletion of Disheveled 3 had no effect [120]. A recent study using zebrafish and Xenopus embryos has identified a novel negative regulator of the canonical Wnt signaling pathway, Kctd15; overexpression of Kctd15 inhibits NC markers, while morpholino depletion causes their expansion. A knockdown of Kctd15 rescues Wnt8.1 morphant zebrafish, suggesting Kctd15 acts downstream of ligand binding to decrease signal strength [121]. Another novel regulator of Wnt signaling is ADAM13, a metalloprotease. ADAM13 was shown to be necessary for cranial NC induction, as morpholinos inhibit expression of Snail2, Sox9, and Twist in Xenopus tropicalis embryos. Here, ADAM13 cleavage of Ephrins B1 and B2 is thought to promote Wnt signaling by preventing inhibitory effects of forward EphrinB signaling, thus allowing sufficient Wnt signaling to activate Snail2 and induce the NC [122]. Non-canonical Wnt signaling may be required for NC induction in Xenopus as well—a recent study suggests a crucial role for Wnt11R signaling from the neuroectoderm. The authors demonstrate Wnt11 is capable of activating PAR-1 (also known as microtubule-associated regulatory kinase—MARK) and both molecules are required for early NC markers (Pax3, FoxD3, Sox8), independent of the β-catenin pathway [123].
Interestingly, a recent study using chick explants proposes Wnt signaling mediates the temporal activation of BMP signaling necessary during the second step of NC induction. Treatment of prospective neural explants with Wnt3a at gastrula stages upregulates Bmp4 expression and can induce NC markers, while treatment of prospective NC explants with a Wnt inhibitor causes a loss of NC markers and a downregulation of Bmp4 levels [30]. If this same regulation is also present in Xenopus, it would explain why animal caps treated with BMP antagonists and Wnt ligands undergo NC induction: BMP antagonists and Wnt ligands promote the early induction of NPB markers, and then the Wnt ligands activate BMP signaling, overpowering BMP antagonism and leading to the expression of NC specifiers.
Fibroblast growth factor signaling
Fibroblast growth factors (FGFs) comprise a large family of secreted polypeptides (22 genes in vertebrates) that bind to transmembrane receptor tyrosine kinases called FGF receptors (four genes, FGFR1–4 in vertebrates) with the assistance of extracellular matrix components, notably heparin sulfate proteoglycans. Following ligand binding, the receptors dimerize and transphosphorylate one another and activate one or more intracellular signaling cascades, including those mediated by Erk1/2 (MAPK), PKC, and PLC-gamma. Alternative splicing has been reported in several ligands as well as FGFR1–3, adding to the complexity and specificity of ligand/receptor interactions and downstream signaling. FGFs have been implicated in multiple aspects of early development, including mesoderm and endoderm formation, gastrulation movements, anterior-posterior and dorsal–ventral patterning, and neural induction among others (for a review, see [124, 125]).
Several Xenopus studies have demonstrated the importance of FGF signaling, with a specific focus on Fgf8a as a NC inducer. This spliceform of Fgf8, unlike Fgf8b, has little to no involvement in mesoderm development [126]. Fgf8a is expressed in the DLMZ at gastrula stages, but becomes restricted to more posterior tissues at later stages of development [49, 50]. An Fgf8a morpholino inhibits expression of Msx1, Pax3, Hairy2, Snail2, Sox8/10, and Ap2 [3, 4, 11, 49], and unlike Wnts, Fgf8 is capable of transiently inducing NC markers in an animal cap assay without additional BMP inhibitors [50]. Interestingly, overexpression of Fgf8 in low doses expands the expression of numerous NPB and NC specifiers, but in higher doses actually inhibits them [3], suggesting a specific threshold of FGF activity is necessary for NC induction. A recent Xenopus study identified a transmembrane protein, Lrig3, which may participate in modulating FGF levels [127]. Lrig3 seems to enhance Wnt signaling, but inhibits Erk1/2 activation and the NC-inducing activity of FGF ligands, potentially though an interaction with FGFR1. Morpholino analysis demonstrates that Lrig3 operates downstream of Pax3 and Zic1 but upstream of NC specifiers Snail2, FoxD3 and Sox9, and could act in the transition from NPB specification to NC specification.
In Xenopus, FGFs are likely to act during gastrulation and recent experiments suggest the role of FGF is indirect, acting on mesodermal tissues to induce Wnt8 expression [49]. The authors show that Fgf8 overexpression is unable to rescue the loss of Snail2 and Sox8 in Wnt8 or β-catenin morphants, but overexpression of Wnt8 or β-catenin can rescue NC deficiencies brought about by an Fgf8 morpholino. Furthermore, the combination of Chordin and Fgf8a in an animal cap assay will induce Pax3, Snail2, and Sox8, but addition of a Wnt8 morpholino blocks induction. They also demonstrate that Fgf8a overexpression can expand the domain of Wnt8 expression and that Fgf8 morphants lack Wnt8 expression in the mesoderm at late-gastrula stages. A previous study, however, instead suggests that FGF signaling acts directly on the ectoderm; conjugates of DLMZ and animal caps present strong expression of Snail2, FoxD3, and Sox9, but conjugates of DLMZ with animal caps injected with a dominant-negative FGFR1 do not [50]. A similar experiment using a dominant-negative FGFR4a did not cause a loss of NC markers, suggesting that signaling through FGFR1 is a key modulator of NC induction in the ectoderm. In support of a potential ectodermal requirement for FGF signaling, conjugates of neural and epidermal tissue express Snail2, but when the neural portion is injected with dominant-negative FGFR1, the conjugates no longer express Snail2 [61]. Moreover, Wnt3a has been implicated in the ectodermal expression of Meis3, a factor capable of directly activating transcription of Fgf3 and Fgf8 in animal cap assays, suggesting FGF may be activated in the ectoderm [7, 9].
A recent study demonstrates an ectodermal requirement for FGF signaling in chick as well [76]. In this work, inhibition of FGF signaling during gastrulation via electroporation of a dominant-negative Fgfr1 or Mkp3 (inhibitor of MAPK signaling) causes a loss of Pax7 and Snail2, but treatment after gastrulation causes no effect. Using epiblast explants and by restricting electroporation to the prospective NPB, this study demonstrates that FGF/MAPK signaling within the gastrula epiblast is required for NC induction. Interestingly, FGF receptors 1 and 4 are expressed in the prospective NC epiblast during this time, but are not found in the mesoderm [76, 128], suggesting FGF does not act on the mesoderm in avian NC induction. FGF signaling is necessary for mesoderm formation [129], however, and many FGF ligands are expressed in the mesoderm and primitive streak (http://geisha.arizona.edu/geisha; 130]). During the stages leading up to gastrulation, Fgf8 is expressed in the hypoblast (the tissue underlying the epiblast), and is thought to participate in neural induction [131], making it an attractive candidate to act on the prospective NC epiblast. Still, the source of the inductive ligand has not yet been identified in the chick, and other FGFs including Fgf3 are expressed in the epiblast itself [132]. The highest level of FGF/MAPK activity during gastrulation is found in the primitive streak [76, 128], and during this and later stages, it is known to regulate the expression of multiple Wnt ligands expressed there, including Wnt3a, Wnt8a/c, and Wnt5b [76, 129, 133]. However, FGF was also found to positively regulate antagonists of the canonical Wnt signaling pathway including NOTUM, Sizzled, Sfrp2, and Cerberus [129]. It would be interesting to determine whether FGF/MAPK regulation of these molecules is necessary for NC induction in addition to its activity within the prospective NC epiblast itself.
A requirement for FGF signaling in zebrafish NC induction has not yet been proven, but FGF/MAPK signaling is crucial for dorsoventral patterning during gastrulation and overexpression of Fgf8 causes an expansion of Ap2 [134, 135]. In the mouse, several FGF molecules are known to act early in development, but have not been functionally linked to NC induction. Null mutants for Fgf4 [136] and Fgfr2 [137] display defects in the inner cell mass, and knockouts for Fgf8 [138, 139] and Fgfr1 [140, 141, 142] do not gastrulate properly and thus die before NC formation. Studies have not yet assessed later contributions of these molecules, but all other FGF knockouts generated so far appear to undergo normal NC induction (see [143] for a summary).
In addition to its ability to modulate the Wnt pathway, FGF/MAPK signaling also contributes to BMP antagonism on multiple levels. In Xenopus and zebrafish, FGF signaling positively regulates the expression of Chordin and Noggin during gastrulation [144, 145, 146], and negatively regulates BMP ligand expression in the chick and zebrafish [114, 132, 135]. A recent study also shows FGF positively regulates SNW1 in the chick [129], a molecule thought to modulate BMP signaling in Xenopus [52]. Additionally, a compelling intracellular regulation of Smad1 has been uncovered, directly linking MAPK signaling to Smad inhibition [147]. MAPK was found to phosphorylate the linker region of Smad1, leading either to Smurf1-mediated polyubiquitination and degradation or exclusion from the nucleus [147, 148, 149]. This pathway was shown to be crucial to Xenopus neural induction [148, 150, 151], and may operate similarly to cell-autonomously regulate Smad activity in the prospective NPB. Even more intriguing, GSK3, active in the absence of canonical Wnt signaling, was also shown to phosphorylate the Smad1 linker, downstream of MAPK phosphorylations [148]. This provides yet another mechanism whereby Wnt signaling could promote BMP signaling. These findings suggest Smad signaling is a platform to integrate signals from the BMP, Wnt, and FGF pathways. If this is found to be conserved across species, it could account for the reported formation of NC in zebrafish lacking dorsal BMP antagonists (Chordin/Noggin/Follistatin) [53].
Notch signaling
Notch proteins are transmembrane receptors activated by binding to transmembrane ligands on the surface of adjacent cells. Following ligand binding, multiple cleavage events occur, leading to the intracellular release of the Notch intracellular domain (NICD). The NCID then translocates to the nucleus where it converts the recombining binding protein suppressor of hairless complex from transcriptional repressor to an activator with the help of proteins from the mastermind-like protein family. The NCID is also able to participate in additional transcriptional activation processes, independent from this canonical pathway. Notch signaling has been implicated in numerous developmental processes, particularly involved in establishing boundaries between different cell types (see [152] for a review).
Studies from Xenopus demonstrate a crucial role for Notch/Delta signaling, but the precise time of its activity is still uncertain. Glavic et al. show that Notch is expressed in the prospective NC territory, while ligands Delta and Serrate are expressed in the surrounding regions. They propose that Delta1 interacts with Notch to activate the transcription factor Hairy2, which then suppresses Bmp4 signaling, allowing the inductive phase to proceed [8]. However, this suppression appears to occur during the second maintenance phase, when BMP signaling must be activated. A more recent study using the same tools reports contradictory results, perhaps due to slight differences in the stage of treatment. This group shows that Hairy2 is not regulated by Notch, but is positively regulated by BMP inhibition, the canonical Wnt pathway, and Fgf8, and is downstream of Msx1, Pax3, and Zic1. They propose that Hairy2 maintains NC progenitors, as overexpression represses NC markers and upregulates NPB markers [11]. A follow-up study finds that Hairy2 actually activates the Notch pathway cell-autonomously, activating Delta1 via STAT3. Delta1 then acts non-cell-autonomously to upregulate Id3, Snail2, and Sox9 [12]. This suggests Hairy2 may act as a trigger for Notch/Delta signaling at the maintenance phase, eventually leading to the expression of NC specifiers. Another Xenopus study extensively characterizes a novel secreted protein, Tsukushi (Tsk), necessary for NC induction. Tsk is capable of acting as a BMP antagonist through direct binding to Bmp4, while also regulating the Notch pathway by binding to the extracellular domain of Delta1. The authors propose Tsk is essential to establish the proper level of BMP signaling at the prospective NPB during gastrulation, together with Notch signaling [153]. Potentially, Hairy2 and Tsk serve to modulate Notch and BMP signaling at multiple levels of NC development, but more research is needed.
In the chick, one study has assessed Notch signaling, demonstrating a role in refining the NC domain after induction has taken place. In this instance, Notch seems to act indirectly. Both overactivation and inhibition of Notch signaling cause an inhibition of Bmp4 in the epidermis and Snail2 in the neural fold, but overexpression of Bmp4 in these embryos can rescue the loss of Snail2 expression. This suggests Notch acts primarily to regulate Bmp4 levels [154]. Notch has yet to be implicated in NPB specification during gastrulation in chicks.
Notch signaling has also been linked to NC development in zebrafish embryos, though it seems to act primarily by restricting the neural domain. A loss of Notch/Delta signaling in mindbomb (mib) mutant zebrafish causes a loss of NC derivatives at the expense of lateral NP derivatives such as interneurons [155]. Another study suggests Notch acts via repression of Neurogenin-1 function, restricting neurogenesis without actively promoting NC formation [156]. More recent studies suggest Notch/Delta acts earlier, refining the border of the neural plate specifically through negative regulation of the transcription factor prdm1a (Blimp1) [157]. Prdm1a, necessary for NPB specification in zebrafish, antagonizes another factor olig4, which defines the lateral edge of the NP and promotes neural cell fates over NC [23, 157, 158]. It appears olig4 is restricted by BMP signals during gastrulation, as swirl/bmp2b mutant zebrafish demonstrate a laterally expanded expression of olig4 [158]. Importantly, in all these zebrafish studies, inhibition or loss of Notch signaling primarily affected trunk, but not cranial NC cells, suggesting it is not responsible for the initial induction of all NC cells.
Mouse mutants for members of the Notch signaling pathway generally display an increase in neuronal differentiation markers and a decrease in progenitor markers, demonstrating a critical role in the early stages of CNS development (for a review, see [159]), but there is no support for a role in NC induction. Homozygous null mutants for Delta1 display proper generation of NC cells, but show defects in migration and differentiation [160], consistent with a later role for Notch signaling.
Other signaling pathways
Retinoic acid (RA) signaling has an established role in caudal neural patterning, and may act in NC development as well, though it seems to act after the initial induction. RA signaling is restricted to the caudal portions of the embryo during early development, owing to the posterior localization of the RA-synthesizing enzymes (retinaldehyde dehydrogenases, Raldhs) and the anterior localization of RA-degrading enzymes (Cyp26 family members). A study using Xenopus animal caps showed that induction of Pax3 by chick mesoderm or NP does not require RA [161] suggesting RA is not necessary for NC induction. Another study using Xenopus embryos demonstrated that addition of exogenous RA or over-activation of RA signaling can expand Snail2 expression anteriorly, whereas treatment with a dominant-negative RA receptor causes a posterior expansion [110], suggesting RA effects on the NC are secondary to axial patterning. An avian study using vitamin A-deficient (VAD) quails (which lack the RA precursor) suggests RA is required for the survival of migrating NC cells; VAD quails appear to form cranial NC cells properly, but within a few hours of migration, they undergo extensive apoptosis [162]. A mouse study evaluated RA signaling in the cranial neural crest and provides a slightly different perspective [163]. As a result of knocking out both Cyp26a1 and c1 together, RA signaling is expanded anteriorly, and although NC markers Snail and Sox9 are expressed normally in the cranial neural folds, migrating NC cells are largely absent. Interestingly, crossing Cy26a1/c1 double mutants with a null mutant for Raldh2 (the only RA-synthesizing enzyme present at these stages) rescues the NC migration defect, despite the expected absence of RA signaling in these embryos. This suggests that although over-activation of RA signaling can disrupt cranial NC migration, endogenous RA signaling is not required for migration. Furthermore, because NC markers were initially expressed normally, RA signaling does not appear to function early in NC induction in the mouse.
A recent study evaluates the role of Indian Hedgehog (Ihh) in early neural crest development in Xenopus [164]. Here, the authors demonstrate Ihh signaling is necessary for NC induction, maintenance of progenitors; loss of Ihh function using morpholinos, dominant-negative constructs, and chemical inhibition causes a loss of both NPB and NC specifiers and an expansion of neural and epidermal markers. They reveal requirements for autocrine signaling within the prospective NC and paracrine signaling from the mesoderm, with mesoderm-based signaling further being necessary for proper migration. A well-characterized member of the hedgehog family, Sonic hedgehog (Shh), has established roles in the formation of left/right asymmetry and ventral patterning of the spinal cord, and although ectopic application of Shh can repress NC markers at later stages in the chick [59, 65, 165], an endogenous role in NC induction has yet to be proven.
Lastly, Endothelin signaling has also been implicated in Xenopus NC induction [32]. Using a combination of in vivo and explant approaches, the authors suggest Endothelin-1, released from the mesoderm, functions in both NC specification and cell survival. They demonstrate that morpholino knockdown of the Endothelin-A receptor and chemical inhibition of Endothelin signaling causes a loss of NC specifiers FoxD3, Sox9 and Sox10, but not the NPB specifier Msx1, suggesting a role in NC progenitor maintenance during the mid-neurula stage. With NC induction requiring the precise temporal regulation of multiple transcription factors, additional signaling pathways are surely yet to be uncovered.
Posteriorization and early inducers
Wnts, FGFs, and retinoic acid (RA) are proposed to act as caudalizing or posteriorizing factors during early development. In neural development, it is thought that the NP is initially composed entirely of rostral or anterior character, subsequently being posteriorized by the action of Wnts, FGFs, and RA to give rise to caudal components of the nervous system (reviewed in [166, 167]). Since these same factors are involved in NC induction, it was proposed that the NC is a result of NPB posteriorization [110]. A study in chick, however, indicates the prospective NPB is initially specified by gastrula stages to become NC at all axial levels, requiring the inhibition of Wnt signals at later stages to allow placodal development anteriorly [30]. Additionally, work in Xenopus suggests Wnt signaling acts directly to induce the NC, independent of its role in antero-posterior neural patterning [111]. Recent Xenopus studies identify two factors, Gbx2 and Meis3, as direct targets of Wnt/β-catenin signaling necessary for NC induction [7, 10]. Although both of these genes are involved in caudal neural patterning, the NC-inducing activity of Gbx2 is separable from its role in NP posteriorization [10]. Gbx2 is also shown to cooperate with Zic1 to specify the NC fate, while Zic1 activity alone leads to placodal development [3, 10]. These findings argue that NPB antero-posterior patterning and NC induction are distinct from events in early neural development.
Although these studies describe Gbx2 and Meis3 as some of the earliest-expressed proteins necessary for NC induction and direct targets of Wnt signaling, another recent study proposes Ap2a is the earliest-acting factor in Xenopus [6]. Ap2a is broadly expressed, including the prospective NPB from the onset of gastrulation (stage 10) and preceding the expression of all other NPB specifiers but still downstream of Wnt/β-catenin signaling. They demonstrate that morpholino depletion of Ap2a causes a loss of Msx1, Hairy2, Pax3, and Snail2, and a gain-of-function upregulates them. Although the initial expression of Ap2a is not affected by morpholinos against other NPB specifiers, later expression is. A zebrafish study also suggests Ap2a (Tfap2a) is a critical early regulator of the NC fate, along with FoxD3 [21]. These two factors are expressed during gastrulation and are necessary and sufficient for NC development. Interestingly, double-mutants of Ap2a and FoxD3 actually present altered patterns of BMP and Wnt signaling, suggesting these factors participate in establishing the signaling environment required for NC induction. The expression patterns of Ap2 and FoxD3 in the chick, however, are not consistent with an early role in NC induction [16]. Instead, Pax7 is currently the earliest-expressed factor necessary for NC induction in the chick [14]. This marker is expressed exclusively in the NPB soon after gastrulation and later labels the entire neural folds. This contrasts with other NPB specifiers that are found more lateral (Msx1, Pax3) and NC specifiers, which are initially only expressed in the cranial NC (Snail2, FoxD3, Sox9) [16].
Neural and neural crest induction: shared first steps?
Recent studies in the chick and frog describe the initial induction of the NC taking place during gastrulation, requiring the activation of FGF and Wnt signaling and the inhibition of BMP signaling. Studies from these same organisms suggest neural induction also requires BMP inhibition and the activation of FGF/Erk signaling from the blastula to the gastrula stage [114, 131, 132, 168, 169, 170] (reviewed by [171]), prompting the question of whether neural and NC cells are initially specified together.
In the chick, Erk1/2 proteins are activated throughout the epiblast, encompassing both the prospective NP and prospective NPB from blastula to gastrula stages [76, 128]. Although studies of NC induction have not analyzed effects of FGF/Erk inhibition at pre-gastrula stages, inhibition at gastrula stages causes a loss of NPB markers in their endogenous domain, but an upregulation in the NP [76]. Thus, if FGF is to act similarly on these populations, it likely does so before gastrulation. Furthermore, activation or inhibition of Wnt signaling can interchange the expression of neural and NC markers in explants taken at pre-gastrula [30, 114] but not post-gastrula stages [55], suggesting Wnts mediate the initial choice between these fates before gastrulation. Indeed, Wnt ligand expression and nuclear β-catenin are found in the lateral epiblast, but absent from the medial, neural-specified epiblast at blastula stages [114, 115, 172]. Furthermore, in Xenopus, inhibition of Wnt signaling causes a strong expansion of neural plate markers at the expense of NC and placodal markers. Yet, upregulation of canonical Wnt signaling interferes with neural induction, and this activity can be separated from Wnt-mediated NC induction [107].
In Xenopus animal cap assays, addition of BMP antagonists such as Chordin or Noggin is sufficient to induce neural markers (the animal caps are said to be "neuralized"). To launch and maintain NC markers in this assay, caps must first be neuralized and then treated with FGF or Wnt agonists, supporting a potential shared requirement for BMP antagonism. However, BMP antagonism seems to behave differently toward markers of neural and NC; inhibition of BMP signaling can induce ectopic neural and NC markers prior to the blastula stage, but inhibition at early gastrula stages only generates ectopic NC [173]. Similarly, inhibition of BMP signaling in lateral ectoderm of the chick at gastrula stages can induce ectopic NPB and NC markers [174], but not neural markers [169]. Interestingly, BMP ligands are expressed throughout the epiblast at blastula stages [132, 175], despite the seeming requirement for BMP inhibition to initially promote both neural and NC fates. Perhaps signaling at the blastula stage in chick is primarily utilized to maintain the pluripotency of the epiblast, similar to the situation in the mouse (discussed below).
Early signaling insights from mouse studies
Although the BMP, Wnt, and FGF signaling pathways are needed to establish cell fates at gastrula and neurula stages, studies of mouse embryonic stem (ES) cells (derived from the inner cell mass of the blastocyst) and epiblast stem cells (EpiSCs) suggest dynamic and temporally segregated roles earlier in development. The FGF/Erk signaling cascade must be inhibited to allow self-renewal and pluripotency in mouse ES cells, with activation of the pathway driving them towards differentiation (summarized in [176]). Along with Erk inhibition, GSK inhibitors are required to retain a pluripotent state, but this activity seems to be independent of the Wnt pathway. Alternatively, leukemia inhibitory factor (LIF) and Bmp4 are capable of maintaining mouse ES cells in an undifferentiated state. Autocrine Fgf4/Erk signaling appears to push mouse ES cells towards differentiation [177] and Erk signaling seems to be necessary for their adoption of the neural fate [170], but an in vivo study of mouse development suggests FGF signaling is not necessary for neural induction [178]. Studies of earlier development support an endogenous role for BMP signaling [178] and FGF/Erk inhibition [179] in maintaining pluripotency of intact embryos, suggesting the initial differentiation activity of FGF may be sufficient to establish the competence of the epiblast for neural development. In support, the addition of exogenous FGF ligands is essential for the self-renewal and pluripotency of EpiSCs (derived from the gastrula epiblast), with inhibition of the FGF/Erk signaling leading to differentiation [180]. This is in stark contrast to mouse ES cells where FGF/Erk signaling provides the opposite instructions. Indeed, FGF signaling in EpiSCs actually serves to inhibit neural induction [181]. Together with ES cell studies, these findings suggest FGF has an initial role in epiblast formation, but then serves to maintain pluripotency of the epiblast [182]. These changing roles of FGF signaling caution us to consider temporal differences in cellular responses and underscore the dynamic nature of signaling pathways.
Human neural crest development
Advancing our limited understanding of human NC development will surely improve our capacity to address the many human maladies associated with improper NC development. Model organisms have provided invaluable information on NC development and while many molecules and processes are conserved (as described above), the deviations that are present make it imperative to specifically study human NC biology. The morphology of early human NC development has been depicted from careful histological analysis [183]. An extensive molecular profiling study was performed on cell lines derived from human neural tube explants, presumed to be NC, and indicates the human NC shares many markers with stem cells. Additionally, the study suggests conservation among NC cells of the chick and mouse, but also points to unique traits in the human NC [184]. More recently, a battery of NC markers were analyzed in cranial and trunk regions of intact early human embryos (Carnegie stages 12–18), confirming a broad conservation of expression profiles with model organisms [185]. This study identified Pax3, Sox9, and Sox10 expression in pre-migratory NC at early stages of caudal trunk development with Ap2, Pax7, Sox9, and Sox10 expressed during early migration at more anterior locations. Of note, HNK-1 identified few migratory NC and while p75 recognized many more, it only labeled a subset of NC cells. More importantly, p75 also labeled many non-NC cells. This is particularly relevant, given the broad use of these two markers amongst stem cell biologists (see below).
Given the obvious experimental limitations and restrictions of studying early human development, human embryonic stem cells (hESCs) provide an excellent alternative to study the human NC. While it is difficult to argue how close this system replicates embryonic development, it does have the capability to challenge the potential of cells in many different contexts and to expose used mechanisms and restrictions. hESCs can be induced to form NC-like cells capable of differentiating into nearly all known NC derivatives. These progenitor cells have been generated by several different protocols in varying culture conditions [186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198]. Initial work relied on stromal cell co-cultures [186, 188, 191], and later progressed to derivation from embryoid bodies [187, 198] or neurospheres [192, 193, 196]. Cleaner and more efficient protocols have been recently reported to derive NC from hESCs in cultures of adherent cells in serum-free defined media, without complex intermediary structures [190, 194, 197].
Culture density of hESC was reported to alter neural and NC formation in a differentiation protocol including FGF2, Insulin, and gradual exposure to both BMP and Nodal/Activin inhibition (Noggin and SB431542) [190, 194]. Another study demonstrated that both BMP and Wnt signaling were necessary for NC markers in cells derived from neural rosettes, but it was unclear whether these signals were required for the initial marker expression, or for their maintenance and the generation of derivatives [193]. In several of these pioneering studies, HNK-1 and p75 were used to screen for NC progenitors, but recent findings highlight complications in the use of these markers. In the human, p75 marks only subsets of NC and additionally labels many non-NC cells, while HNK-1 labels a smaller fraction of NC [185], and in at least one study, their use was shown ineffective to discriminate between NC and non-NC [193]. Still, HNK-1-positive, p75-positive cells induced from hESCs do exhibit the capacity to generate an array of derivatives characteristic of the NC. In some of these studies, NC-like cells arise from Pax6+ neural precursors, while in others, an alternative origin has been proposed. Yet, no studies have addressed their possible equivalence or distinct differentiation potential. An intriguing recent study reports a direct protocol for the generation of NC progenitors from hESCs in 12–14 days using a GSK3-β inhibitor (BIO) to activate canonical Wnt signaling, Noggin to inhibit Smad1/5/8 signaling, and SB431542 to inhibit Smad2/3 signaling [197]. Interestingly, removal of Noggin has no effect, perhaps owing to the low level of basal Smad1/5/8 activation, but addition of BMP4 suppresses the generation of these cells, suggesting high levels of BMP/Smad signaling are not conducive to the formation of NC-like cells from hESCs. These conditions mimic some of those currently thought to induce the NC in the model organisms studied, and underscore the value of continued research in NC induction. Reciprocally, the study of hESC biology will undoubtedly unveil exciting new insights into the signaling events in NC development in vivo.
Summary of inductive signals
The first phase requires at least a partial attenuation of the BMP/Smad signaling pathway, but by neurula stages BMP signaling must be activated. BMP inhibition is crucial for both neural and neural plate border specification, and might be regarded as a required step in establishing the competency of the prospective ectoderm. BMP attenuation is likely achieved though multiple redundant methods, including the limited expression of BMP ligands, the activity of secreted BMP antagonists (Chordin, Noggin, and others), and FGF/MAPK-mediated intracellular Smad inhibition. The activation of BMP signaling at neurula stages may be temporally regulated by Wnt signaling. Additionally, the canonical Wnt signaling pathway must be activated within neural crest progenitors themselves and throughout early neural crest development. Analogous to other developmental processes, Wnt signaling is capable of mediating cell-fate decisions, and likely directs the choice between neural and neural crest progenitors. Notch also promotes neural crest development over neural cell fates at multiple points in development, but the precise mechanism(s) remain to be understood. Lastly, a functional FGF signaling pathway is necessary for the initial induction of neural plate border specifiers. However, FGFs are capable of regulating Wnt and BMP signaling and are crucial for several other developmental events (including neural induction, mesoderm development, gastrulation movements, and early epiblast pluripotency and competence), all of which could influence NC induction. In the years to come, the real challenge will be to understand the cross-regulation and combinatorial inputs of these and other signaling pathways, their precise temporal effects, and how they integrate to establish the neural crest program. Since recent studies have found that the NC is specified at pre-gastrula stages, a more thorough analysis of these signaling pathways is called for at early stages of development to understand the complete transcriptional cascade of events that enable the amazing plasticity of the neural crest.
We thank E. Betters for contributing to Fig. 1 and B. Murdoch for comments on this manuscript.
Open Access
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.


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Schools are failing bright children by putting them in for exams too early, Sir Michael Wilshaw, the chief schools inspector, warns today.
Thousands fail to get top grades in their English and maths GCSEs because they are taking them a year early. As a result, they are likely to miss out on studying the subjects for A-level or at university.
Figures produced by Ofsted, the education standards watchdog, show the number of pupils being put in for maths and English GCSEs a year early has shot up over the past 10 years.
Between 2005 and 2012 the number of students put forward for early GCSEs in these subjects has risen from 9,000 to 241,000 in English and from 24,000 to 218,000 in maths.
The figures also show that, of the brightest pupils identified by national curriculum tests at age 11, only 37 per get an A* or A grade pass in English if they take the exam early. That compares with the 49 per cent who get top grades when they take the exam at 16. In maths, the figures are 43 per cent and 54 per cent respectively. Sir Michael, who is also head of Ofsted, said the figures revealed "the perverse incentive of the league tables".
"All heads are anxious about their position in terms of five A* to C grade passes including maths and English," he said. "In some ways this is a perverse incentive: making sure your five A* to Cs are as good as last year and are beating the school down the road."
As a result, schools are "banking" their most talented pupils' C grades a year early and then concentrating in the final year of GCSE preparation in pushing them through to C grades in other subjects. This approach could lead to them receiving no English or maths teaching in school after the age of 15, Sir Michael added.
Sir Michael also blamed "the curse of mixed ability classes without mixed ability teaching" for failing pupils. He said it was hard to cope with a disadvantaged pupil with poor skills sitting next to a "potential Oxbridge graduate" in the same class.
About 20 per cent of those in the top level at age 11 failed to get the A*, A and B grade passes their talent warranted, he said. "It is a combination of low expectations about what these youngsters can achieve and the curse of mixed ability classes without mixed ability teaching," he said.
"Heads have got to make up their minds. If they want mixed ability they've got to ensure there is good teaching. It must not be just an article of faith – comprehensive means mixed ability." Sir Michael said 6,000 schools teaching two million children needed to improve from their current ranking of satisfactory – now abolished under new inspection procedures – to good within four years.
"About a third of our schools are less than good," he added.
A spokesman for the Department for Education said: "Candidates entered early often perform worse overall than those who do not, even after re-sits are taken into account.
"Some pupils are being entered before they are ready and 'banking' a C grade but their performance at Key Stage Two [seven to 11] suggests that if they had continued to study the subject and taken the GCSE at the end of Year 11 [at 16] they could have achieved a top grade."
Science grade boundaries also changed
Grade boundaries in science were changed at the last moment at AQA (Assessment and Qualifications Alliance), it emerged yesterday. The pass marks for A*, A, B and C grades in chemistry and biology were lowered between the January and June sittings. Less than 1 per cent of biology candidates achieved an A* grade in January compared with more than 10 per cent in June. In chemistry the respective figures were two and 12 per cent. However, the lowering of the C grade in biology led to fewer candidates achieving it.Reuse content


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text: SHANGHAI, April 3, 2013 /PRNewswire/ -- Swedish innovator Plantagon International, pioneer in innovative vertical urban agricultural solutions, is setting up the Tongji-Plantagon Research Center with Tongji University, Shanghai. (Logo: http://photos.prnewswire.com/prnh/20121004/567112-a ) (Photo: http://photos.prnewswire.com/prnh/20130403/607128 ) Under the auspices of His Majesty King Carl XVI Gustaf of Sweden, the Consul General of Sweden in Shanghai Ms. Viktoria Li officially opened the lab during a formal ceremony at Tongji University on April 3rd, 2013. Vice President Mr. Li of Tongji University and Mr. Pettersson, COO of Plantagon International together with eminent officials from COFCO, Roosevelt China Investment Corp., SWECO, CENTEC, IKEA and the cities of Baoshan and Wuxi were present to show their support. "We are delighted to extend our collaboration with Tongji University and the Tongji-Plantagon Research Center shall pave the way for the long-term intent we have for this market. We look forward to many constructive results to be developed from this successful collaboration," says Hans Hassle, CEO of Plantagon. The achievement made by Plantagon and Tongji University supports and acts on the articulated objectives of the 12 th five-year plan. E.g. China is dedicated to increase their investment in R&D of CleanTech to over 2 %. Food security and -safety, as well as the problem with contaminated land, are other crucial issues for China to manage in the future. "The status of being a developing country is a great opportunity for China, rather than being "developed". It means that we still have many chances to redefine our future. One of the key issues of today is about the food. The Tongji-Plantagon Research Center is an important concrete step forward towards engaging more people from the city to the agriculture and bridge the urban and rural ways of living and producing," says Vice Dean Professor Lou Yongqi. Plantagon


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Fabuloso Firenze with Fabio'n'Rosalinda
... it is almost anxiety inducing, we decided just to focus on the " big hitters " and a lovely lunch !!
First stop ,Florence's beautiful Duomo/ Catthederale Santa Maria Del Fiore is a masterpiece. It dominates the skyline and has the largest Cupola in Italy, topped with a shimmering gold cross. .......Beautiful. It was a rainy day, which stopped us climbing the dome , however the rain was fantastic for photos as Noel was snapping away at the ...
The New Normal
... went on to Pisa and the weather was clear there and it was much warmer. Of course we took the silly Leaning Tower of Pisa pics and then got gelato and wandered the little town. Around 6:30 pm we finally returned to Florence after an exhausting and exciting weekend. I was able to get all my work done and FaceTime with Mom!! Very grateful for the excursions API provides us with! That was a great first one!
Today, Monday September 14th, I had class at 9 am and then again ...
Accademia and the Bargello Museum
... to the Florentine explorer Giovanni da Verrazano who was first European to explore the Atlantic coast of North America including the New York bay.
We return to our Hotel and prepare go the Church of San Miniato on the other side of the Arno River before dinner. The church is named Saint Miniato after an Armenian prince serving in the Roman army under Emperor Decius. He was denounced as a Christian after becoming a hermit and was brought before the Emperor who ...
... On one hand, I am very excited to be back home with my family and friends. But, on the other hand, this has been the experience of a lifetime. I will never forget my experiences in Europe for as long as I live. But, life goes on. On Friday morning, I fly out of Florence and arrive in Chicago at around 4 P.M. With that said, I wish you all a great rest of the week. I hope you enjoy my pictures and I will talk to you next from the United States.
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Seduced by Florence
... rebuilt or restored to look older than she is. Her crowning feature the Duomo has not been destroyed and redesigned in the 20th century like other buildings around the world. Her past is her present and her present is magnificent. Looking from these high vantage points I am drawn to simple thoughts of human existentialism. Basic, unanimous conditions that we all (even the greats of yesteryear) encounter. Under the red-tiled roofs I can imagine the love and the loss; ...


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The 13,300-square-foot SCADshow features two stages with a total of 560 seats. Built in 1986 as the Academy Theater, SCADshow serves as home to aTVfest and other SCAD Atlanta events. The mainstage has a 375-seat capacity and stage 2 a capacity of 185.
SCADshow offers two spaces for rental: the Mainstage, which has a 365-seat capacity, and Stage 2, which seats 180.
To book an event at SCADshow, please submit a SCADshow rental application. Details on rental rates and application are available under the theater information tab located above.LEARN MORE>


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Big data helps movies predict hits
Technology service provider Paipianbao gained 4,500 cinema subscribers throughout China
Zhang Xiaoming is not a big fan of movies but he can tell which films will be a big hit in China and which will not by using an advanced technology named big data.
"With enough data, many things can be predicted, even the stock market index," said Zhang, who had around 20 years of experience in data analysis in the United States before heading the big data research center at Zhijiang College of Zhejiang University of Technology in 2011.
In Shaoxing, a city in eastern China's Zhejiang province where the college is located, Zhang and his 18-member team work on big data solutions that cannot only help producers know which combination of cast can bring more box office sales before shooting, but can also help cinemas make more money by smartly arranging the screening of movies.
"Around 80 percent of the movies produced in China cannot get noticed by cinema managers due to their poor quality. Less than 10 percent of movies can actually make a profit," said Zhang, adding that it is important to use technology to help producers and cinemas in decisionmaking so that they can maximize their profits.
Zhang, who received his PhD and master's degree from theMassachusetts Institute of Technology, said by using cinemas' previous ticketing data, data from the media and the public's reviews of movies combined with the algorithm they developed, his team can predict the box office and increase the number of moviegoers to cinemas by 10 percent to 15 percent.
He used Hong Kong film director Stephen Chow's latest movie Mermaid as an example. "The movie was about to release with two other movies during the Spring Festival. All of the three movies seemed equally good by any standards," Zhang said.
"But one local cinema listened to our advice to use its biggest screening room to showMermaid, and it earned hundreds of thousands of yuan within a week," he said.
It turned out that the Stephen Chow's comic fantasy not only beat locally made MonsterHunt andHollywood action movie Furious 7 during the same period, but became the first Chinese movie to reach the new landmark of 3 billion yuan in gross receipts in China.
Launched about 18 months ago, Zhang's big data service named Paipianbao has gained 4,500 cinema subscribers. China has a total of 7,000 cinemas.
The majority of cinemas hire experienced managers to draw up their screening calendar, deciding which movies will be shown in which room at which time. With Paipianbo, the calendar can be automatically compiled within one minute.
"The service replaces the role of cinema managers to some extent. The most difficult aspect of further expanding our business is how to convince more cinemas to adopt our technology," he said.
He said that his service has the most accurate projection on box office so far but there are other players which want to replicate his success. "I don't think their projection can be as accurate as ours. But box office prediction is not a 100-meter race, even if you run faster than your competition by seconds, it doesn't make a big difference," he said.
Contact the writers through mengjing@ chinadaily.com.cn


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Chief Bryan LaForme is unhappy with the War of 1812.
The rest of Canada may consider the conflict a thing of the past, and be largely immune to government-sponsored commemorations meant to boost the war's image.
But on the Mississaugas of the New Credit reserve near Hagersville, Ont., within the confined 2,500-hectare homeland of an Ojibwa people who once roamed much of Southern Ontario, history persists in the present tense.
"For me, the war is a disappointment," Mr. LaForme says over a morning coffee at the native-owned Country Style Bistrodeli, as Stanley Cup highlights flash on a nearby TV. "It's always about the British and the Americans, with no mention of the First Nations. So we were virtually invisible until a few of our people made this a focal point for us and got some recognition for our contribution – which is nice, but about time."
History, at least when defined by other people, glossed over the Mississaugas in the two centuries since they fought at the battle of York, defending a territory that used to be theirs on behalf of an ally who betrayed their trust. They were paid a pittance for their appropriated land, which includes Toronto and Mississauga, the sprawling conurbation named after its displaced native people.
But on Saturday, the New Credit people are finally putting history in its place. To the sound of beating drums and prayers recited in the long-neglected Ojibway language they are now reclaiming, Mr. LaForme will lead the opening of a 12,000-square-foot community centre that symbolizes New Credit's refusal to remain invisible.
That spacious gathering place was built with funds from a $145-million land-claim settlement with the federal government in 2010 over the invalid purchase of the disputed Toronto-area territory. As part of the grand opening, an exhibit created with the City of Toronto will also be unveiled. Its less-than-conciliatory title? Outcome of the War of 1812: First Nations Betrayed.
"They promised us a lot and the end result was we got squat," says Mr. LaForme, as he ticks off 1812 battles where Chief Joseph Sawyer led his anonymous warriors. "The loss of land, that's what we got."
The Mississaugas proved less useful in peace than in war, despite efforts to assimilate to the newcomers' ways. Threatened by the encroachment of non-natives, the New Credit people's ancestors abandoned their settlement at the mouth of the Credit River in modern Mississauga and moved to their landlocked parcel on the edge of the Six Nations reserve, 40 kilometres southwest of Hamilton.
And 200 years later they finally get thanked. As he inspects the new community centre, the chief comes across a shiny object sitting in a display case beside a collection of millennia-old native artifacts. It's a War of 1812 medallion, recently presented to the New Credit people by the Governor-General.
"In Defence of Canada," he says, reading the inscription with skepticism. "But Canada wasn't even Canada back then."
He's more enthusiastic about a replica Grey Cup on display. As part of the 2012 CFL championship in Toronto, Mr. LaForme conveyed the trophy from Union Station to city hall – one of the symbolic ways the New Credit people have asserted their visibility in a place they still consider theirs, the $145-million settlement notwithstanding.
The New Credit people – who number 600 on the reserve and another 1,600 off – are now trying to take a lead role in the Pan Am Games, which Toronto will play host to in 2015. But, says Mr. LaForme, "the Pan Am group really don't understand the link between us and the land and our traditional stories."
This aboriginal view of shared land tenure has never squared with the non-native idea of real estate exclusivity. "It all starts from a basis of mutual incomprehension," says historian Donald Smith, who is launching his book, Mississauga Portraits, at the New Credit community centre. "The First Nations believed they were lending the use of their land. The newcomers felt it was an outright handover. The expectation was that these people would eventually disappear: They would either be brought into the larger society or perish."
History has finally proved the colonists wrong: Two centuries later, the New Credit people are thriving on their Toronto connections. "The idea I'm always trying to push," says former chief Carolyn King, "is that we were there, and we're still here."
The past is easily lost in a ruthlessly modernizing city like Toronto, where memory is obliterated by social change. "In another 20 years," Mr. LaForme says, "the people governing us are going to be immigrants who have no understanding of the First Nations."
Which is why Ms. King has established the Moccasin Project – native sites across cities and suburbs will be publicly identified with a moccasin symbol representing the tribe that inhabited each location.
"From this point forward, people will know whose land they're on," she says. "And that will go a long way to address the hurts of the First Nations. Because for most of the time, we have just been ignored."Report Typo/Error
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