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Red sonja
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"I have never actually had a plan for my career at all. When I was writing that column, it was very satirical of the comics industry and made fun of all the most powerful players… if I'd been hoping to find a way to break in, that would have been possibly the worst imaginable way to do it. And it followed the Women In Refrigerators site I spearheaded, which I'm sure made me even less popular in some quarters." --Gail Simone
Red Sonja
Red Sonja #2 (Issue)
Inktober Day 20 - Red Sonja by DerekLaufman.deviantart.com on @DeviantArt
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Red Sonja 15B Boeken / Comics, Comics, Red Sonja www.detoyboys.nl
Cool Art: 'Red Sonja #1' by Fiona Staples haha its "me"
BLOG DOS BRINQUEDOS: Red Sonja She-Devil
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"To say I owe my life to IM would not be an understatement"
About: Inclusion Matters Sefton Inclusion Matters Sefton Bootle L20 4TQ
Posted by Thursday (as ),
The help, support and guidance I received throughout my treatment was excellent. Sarah showed sympathy and empathy in all our sessions together.
To say I owe her my life would not be an understatement.
Response from Elizabeth Forrest, CBT Therapist, Inclusion Matters 3 years ago
Dear 'Thursday',
Many thanks for taking the time to leave your feedback with us.
I'm glad to hear that you received the help and support which you needed - it sounds as though you have benefited from it a lot.
I will make sure your positive feedback is passed on to Sarah.
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subset: commoncrawl


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I am the sole shareholder of a small software development company in Swansea. I'd like to bring in a colleague as a full partner, and so need to give him a share. How do I go about adding a shareholder, though? What's the procedure like? Thanks
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- The HomeSteps Winter Sales Promotion will pay $1,000 bonus to the selling agent and a $500 bonus to listing agents who sell HomeSteps homes in select states that buyers purchase as their primary or secondary residence. Two-thirds of HomeSteps homes are sold to owner-occupants.
- Bonuses are available on all HomeSteps homes in select states when approved offers received between November 15, 2012 and February 28, 2013 close and settle on or before April 15, 2013.
- The HomeSteps Winter Sales Promotion is limited to HomeSteps homes sold in Alabama, Colorado, Iowa, Illinois, Indiana, Kentucky, Louisiana, Massachusetts, Michigan, Minnesota, Missouri, North Carolina, Ohio, Oregon, Pennsylvania, South Carolina, Tennessee, Virginia, Washington, and Wisconsin.
- The Winter Sales Promotion does not include investor purchases, auction sales, sealed-bid sales or bulk sales.
- HomeSteps homes sell for an average of 95 percent of current market value. As of September 30, 2012, HomeSteps had an inventory of 50,919 homes nationally, of which an estimated two-thirds were in the 20 states where the HomeSteps Winter Promotion is available.
- For HomeSteps Winter Sales Promotion details and conditions, see http://www.HomeSteps.com.
MCLEAN, Va., Nov. 15, 2012 /PRNewswire/ -- HomeSteps ®, the real estate sales division of Freddie Mac (OTC: FMCC), today announced a special Winter Sales Promotion for selling and listing agents who sell HomeSteps homes to qualified buyers in 20 states between November 15, 2012 and February 28, 2013. News Facts


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Interview: Lily Waronker
Kristina Rose is a West Coast Cali-raised talent, whose fame reaches far across the globe—she tweets to 200,000 followers and has starred in almost 500 adult films. The erotic performer/ dancer and self-proclaimed "LA face with an Oakland booty" has been on our radar for some time now, and not necessarily because of her brilliant rendition of Elaine in the Seinfeld porn parody, or her strip-club feature as a sexy clown—she's known for pulling a long line of handkerchiefs out of her ass. Rose has set herself apart from the bunch with her backwood-rolling, pot-smoking, no-fucks-given attitude.
How long have you been smoking weed?
I used to smoke in high school but my friends didn't like it 'cause they were like, "You're a bitch when you're stoned." And then I didn't really smoke weed again till I was like, I wanna say 23. And I've been smoking weed ever since.
Do you remember the first time you got high?
Yeah, I do…Oh, my God. The first time I got high I was chilling with one of my best friends at the time, a kid named Cody. I can't believe I even remember his name. He was a big dude and a big pothead, and he was like, "Come over, we're going to Black Angus and I got some weed. Let's smoke." I was like, "Alright, I never smoked before," so I was kinda nervous. I'd heard people before say the very first time they start smoking weed they didn't get high, I was so high I couldn't stop laughing. And I was a total bust. His parents were kinda hippies so I think they knew what was up. I think they smoked weed—he probably stole the weed from them.
So we come down there, bloodshot, and his mom is all like "Oh, you guys are awful cheerful. Are you hungry? You wanna get some steak?" Just talking about all this food, I can't stop laughing uncontrollably, like fucking giggling my ass off. Then I went to eat Black Angus with my friend and I came home and my mom was watching Leaving Las Vegas on TV. I was probably like 13 and leaning my elbows in front of the TV, in front of her, on the floor, just being like, "Oh my god, she knows I'm high. She knows I'm stoned." And Leaving Las Vegas is a creepy movie, especially when you're 13 and getting high for the first time.
[Points to a bejeweled Dodgers hat] Did you rhinestone that hat?
Yeah, I did. I did a Dodgers thing and it was so cool because it was the last game before they lost to be in the World Series or whatever. So they were all hyped on it. And San Francisco, I took the Barry Bond's bat—it's a little kid's bat—fucked myself with it. I just fuck myself with everything, that's my magic trick.
That's good. They like it.
Yeah, they do. My ass isn't as big as it used to be so I can't do all the ass-shaking tricks, and I think all the girls do that so it's more like, "Hey look, I can put this in my vagina or asshole." Trying to come up with a new costume and a new gimmick…
How do you think of new gimmicks?
I'll be high or something and I'll just have an idea. Like the Minnie Mouse thing that I did, that started out with this guy that I fuck with on-and-off with forever. He sent me a picture of him with Minnie Mouse and I thought, "Oh, Minnie Mouse, that would be a good idea, dudes like Minnie Mouse." So I made a Minnie Mouse costume cause he was going to come visit me and I was like, "He'll like that, and maybe I'll fuck him in it." All the guys complimented me on that costume so much. I try to get ideas from guys 'cause that's who I'm doing it for really. And girls are usually really into it, too.
One time, a guy brought his iPad—I can't believe they let him. He brought this iPad and he had a banner scrolling, "Kristina Rose is the shit" or something like that across it.
That's great reinforcement.
He brought me weed and stuff; they always bring me weed, I love that. That's why I love letting everyone know I'm a pothead; they bring me weed, they know what I like. I have friends whose agents don't allow them to talk about that. They're always asking me, "How do you get weed? Why do people always bring you weed?" I'm like, "'Cause they know that I smoke it! You need to tell them."
No, Pebbles [Her Chihuahua approaches the weed].
She doesn't get weed.
You don't blow it into her ears?
Isn't that what people do?
Yeah, I just feel like that's mean 'cause they don't really have a say in it. I remember when I was first getting high, that feeling, it's pretty overwhelming, it's pretty powerful. I don't know, I just wouldn't want to do that to someone and they don't have a choice in it.
Thank you.
No, thank you. Sorry if you don't like smoking, but I'll smoke it. I should be a caterpillar [talking about costumes].
Sitting on a mushroom. Why can't you do costumes like that?
I can do whatever I want. I have a big weed leaf costume that I made; it's like a giant weed leaf, and I just put my head in there like that. That one is cool. I was thinking about doing a KISS [band] thing, just because I'm going to Atlantic City and I don't know why that seems very KISS-y to me. It'd be super easy to do and I feel like the crowd in Jersey would totally like it. I try to do different stuff. I used to do like a sports team theme, but then all these bitches were doing the same thing every time. I was like, "Man, I gotta come up with a new gimmick now."
Do people copy you?
I can't really say they're copying me, 'cause I'm definitely not the first bitch that was like, "Oh, I'm going to have a Minnie Mouse costume at this porno shit." It's just probably been many years since anyone has done that. I think some girls in porn just aren't very creative, so they're just like, "What am I supposed to wear to this event?" Then they look around and they're like, "Okay, all those girls are wearing Bebe dresses, so I'm going to go get me a Bebe dress." It's hard to stray from someone's original idea, like some people's costumes and ideas will inspire me, but I try not to copy it; I try to be a little more original than that.
You make your own stuff. That's pretty cool.
Yeah, I have fun with it.


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Prospect Luis Mendoza to start for Royals Sunday
Associated Press | Last updated February 29, 2012
Top prospect Luis Mendoza will start for the Kansas City Royals in their spring training opener on Sunday against the Texas Rangers. Mendoza led the Pacific Coast League with a 2.18 ERA last year. He is scheduled to work two innings, while right-handers Sean O'Sullivan, Vin Mazzaro and Nate Adcock are also scheduled to pitch. Manager Ned Yost said left-handers Bruce Chen, who led the staff with 12 victories, and Mike Montgomery will pitch Monday against the Rangers. Luke Hochevar, the first player picked in the 2006 draft, is scheduled to work two innings of a split-squad game Tuesday against San Diego. Left-hander Will Smith will start the other game that day against Cleveland. Felipe Paulino, who is a candidate for the rotation after making 20 starts last season, has been setback by hamstring tightness and will be held out of the early games and intrasquad games Thursday and Friday.
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Definition from macmillandictionary.com
He was forced to publicly apologize once more for his actions.
The fair was once again a tremendous success.
I returned to Canada and found once again the freedom I had known as a child.
We look forward to the day when there will be peace once more in this troubled land.


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By ADAM SERRANO
The chase for Club Tijuana forward Joe Benny Corona just got a bit more interesting.
Corona has been called up by Mexico's Under-22 side for a pair of matches against Chile's Under-22s. Corona is joined on the roster by another California native, Chivas de Guadalajara left back Miguel Angel Ponce.
Corona has two goals in the Apertura for the Xoloitzcuintles (or Xolos) in their first season since being promoted into the Mexican Primera Division. Corona's rising star began during the Xolos drive for promotion, with Corona even scoring the goal that decided Club Tijuana's promotion playoff. In addition to his ability to represent Mexico and the U.S., Corona is also eligible to represent El Salvador, his mother's birth nation.
In a recent ESPN profile of Corona, Corona stated that he had been on former U.S. national team Bob Bradley's preliminary roster for the Aug. 10 friendly against Mexico but didn't end up making the final roster after Bradley's firing. During his conversation with ESPN, Corona admitted that he has been in contact with the current regime under Jurgen Klinsmann, who sent a scout to watch him in a recent match against Puebla.
What do you think of this development? Confident Corona will wind up with the U.S. national team? Think Mexico is seriously interested, or just trying to keep him from the USMNT?
Share your thoughts below.
Jorge Belon
people calm down i am sorry to say but we have so many other good young players playing in Mexico and in Europe so there is no reason to start freaking out. besides both Ponce and Corona are not lost forever. They were just called to the youth teams so calm down and stop praying for bob to return because it seems like he will be eating cuscus for the next few years to come.
Beside Kinsmann is doing a good job by just watching the game against Mexico. You can tell that our youth will improve way more then they would ever improved under Bob.
oh snap!
Before we start flogging ourselves, where does this guy play in the field? What's his position?
This is All about Money not Allegiences, the Mexican Fed will flush Money at Corona. and potential endorsements the US cant compete at that level. Untill the US has econminc Power of the Mexican team in this country then youll see the tide turn.
"Perhaps if Mexican Americans were made to feel more welcomed in the U.S. (rather than being scapegoated for many social and economic problems not of their making)" WTF does this have to do with soccer?
I for the life of me cannot understand how racism is excepted by you all in the name of US Soccer it disgusting, you do not undo wrongs by discriminating against others. Players should be chosen by their merits and that's it not because of their heritage (which no one has control over)But because of how they play. At the rate we are going we are gonna only pick players based on the census report. I personally don't care what kind of hyphenated american anyone is I want players who can play who can be apart of the core team and who will fight for the shirt. So whether that's a rich white kid from New York or a poor Hispanic kid from Texas if you can play you deserve a shot. But to sit here and say we should only start looking at one set of players instead of another because of their racial background is something that should not be tolerated.
Tim M.
who do some people feel like these kind of articles are an open ticket to explore their racist feelings on america.
Didn't you mean sarcasm?
Jake the Snake
That's true, too bad we didn't look hard enough for Joe when he was here.
Correct, we should incorporate all of the ethnic groups from Hispanics,blacks,whites, and the asians everyone should be able to play, but they must also contribute something to the team.
The ignorants use the internet to create a pool of racism which effects the commenting session.
We can't afford to lose forwards like Corona. Mexico can. The only advantage for US? Mexico's attacking depth at Corona's position. Here's hoping.
Juan from L.A.
Guys he is a goner. He was interviewed by ESPN Deportes guys and his decision has been made. Wish him luck. He is not hte first and won't be the last in choosing Mexico over US or vice-versa. It will happen and YOU ALL in either side have to respect their decisions. The messed up part is Mexico is already ahead in their U-22 development. We don't even have a coach?!?!
Ha ha
I did read your post and it was stupid. How can a 21 year old kid born to central American parents and who grew up in the US and Mexico be expected to simply leap at the US without any thought? Anyone with parents from other countries is likely to be "naturally conflicted," but that shouldn't mean we exclude them on that basis. You know, little things like understanding it is a young kid with a heterogeneous identity would be better then calling for some test of purity. Otherwise, we shouldn't call in any players who aren't completely 'Merican, because they could harbor mixed feelings; they could even like other countries! People, especially young people, in my experience, makes mistakes, like, for instance, accepting a call up for Mexico and then realizing they would like to play for the US, but things like that are evidently too subtle for your magisterial view.
The old "I agree, if you mean what I mean" construct, where a puerile substitution supposedly becomes wit. It's so easy to use even Kevin_amold can do it.
Yup, well said. Many people don't seem to consider the issue of endorsements…
"Construct" and "Puerile Substitution"?! Really? In a casual soccer comments thread? Way to come off better-than-thou. In reality, your reply was simply an overreaction to Joe B.'s post. No one's denying the kid's difficult decision.
Alex G
I´m with you on that one…
Alex G
Where did u hear this news, espndeportes??, this is insane. Who cares, go play with Mex, we will be better again in a while and he´ll come back begging for a chance.
Joe is not spectacular but is very good. If on the USA roster probably places between 20 to 30 another Bedoya.
If on Mexico's roster he places 30 to 40, not likely to play first team soon. However he has a chance at making both countries Olympic teams. His youth coach says Joe
will play with whoever brings him in first.
i really dont understand the need to target a certain ethnicity. why not just call in the best players in the pool. period.
well, let's add to this line of thinking:
in the last friendly
team goes down early? check?
"regular" starters put in an uneven performance? check
teams has to come back relying on fitness and hustle? check
left back puts in a questionable performance? check?
people screaming for the coaches head? um…..
Benny lowe
Most american coaches have a "philosophy" that only 6ft and up players can make it to the NT. When messi the best player in the world stands 5ft 7 could take on 6ft 7 defenders by himself. Seriously that bs needs to stop.
'Best players' need to be brought into the pool, they're not born there, well most are not. Some of these players are dual nationals who need to be courted. Example, if Subotic and Rossi had been courted successfully the USA would have probably made the final 8 or higher at the last WC. That would have been huge for the USA soccer.
So dual nationals need to be looked at when they are very young, not to create an ethnic mix, but to capture a talented player before someone else.
Edwin in LA
Lets be honest, it comes down to styles of play and what parts of the game certain players focus on. Some players might need to work on endurance and physical fitness, things you can actually work on.
But skill and technique is much harder to find and to teach and also to learn. Latin players we all know are more about the possession and nice passing and aggressive offensive football. With no disrespect most of our boys in the US, white or black or Hispanic…. they come from a system of pay to play that focuses on pure great athletes and ride that wave all the way to the world cup.
IMO Torres is WAY better than guys like McCarty, Beckerman, Clark among others oh Larentowicz. Clark being the best of the bunch and then Beckerman far FAR behind.
Gomez I wish people would stop including him in their weird arguments of "mexican rejects" or "conflicted players"
Guy came up thru the US ranks and started in MLS, grew up in Vegas and then in late teens in LA!
He's about as American as they come, go look up Glenn Davis' podcast for Monday's show in the 1st hour of Dynamo Access he talks about his experiences in Mexico and how sometimes he feels just as home, because of the Americanization of a big plaza in the area he lives in.
I too don't think players should be picked based on ethnic background. But lets face it, Orozco has merits, maybe not over John but for sure over Ream and as much as Gonzalez. Torres has plenty of merits, look up game at El Salvador in qualifying.
Castillo, is there for the same reason Heath Pearce played so much and was still considered while sucking in a 2nd Division German team that was being relegated to 3rd, and he couldn't even play for them horrible form!
Eh? So vocabulary should be whittled down to the lowest common denominator?
Edwin in LA
Wow sorry didn't mean to make the post that long?
So Mexico wants him for the U-22 team and some here want him for the senior team. Some guys need to stop being so desperate to chase any player that "might" be good. Players should be waiting to be called by the NT not the other way.
Show a little patience and understanding. Every player's situation is different. It makes sense for someone who's eligible for 2 or more countries to take time to figure out what to do. Why does that bother you so much?
Where did R say anything at all about choosing players based on their heritage?
I agree that Mexican Americans should be made to feel more welcome here. Regardless of the soccer implications, it's the right thing to do. And there is certainly a lot of racism against Mexican Americans.
But, I don't think Jake the Snake's comment qualifies. I read his leftovers comment as meaning that those players might have preferred to play for Mexico, but weren't good enough. It could actually be read as a complement to Mexico and their national team.
Steve C
psssh. We can put Corona in an Allstate commercial and overdub his voice w/ someone who sounds more stereotypically Mexican.
DC Josh
M'eh. This kid scores a couple goals and now he is on everyone's radar. He could be the next Chicharito, or he could be the next Herculez Gomez. We have enough young forwards in professional systems where I feel we don't need to be worried about kids like Corona who pick Mexico over the USA.
Bottom line, Bedoya and Corona would be an upgrade on the wings to Rogers and Kljestan, but not over Landon and Deuce.
why is it that Mexican scouts are always "finding" potential US talent before the US… does the media not report it… or does the US scouts just suck!!!SMH… i usually dont report articles but there is a good article on Soccer America about Mexican club teams casting over US talent.. its a really good read…. why cant our scouts see the talent!!!
There are many interests at stake:
From Mexico's point of view, they are probably interested in this kids talent. I have followed Tijuana for more than a year now and he is KEY to their success. Part of it is also trying to keep him from the US. But remember, he is not caped tied untill he plays a qualifier, even if he does play in the Olympics.
From Corona's point of view, I believe he is sincere is saying he is still open for playing for the US. Mexico called him up first. I wish Klinsmann would still try to call him up on a later date. God knows we could use some good forwards.
But there is one important thing to consider: Mexico's Olympic squad (the same team that went to Copa America) had 8 of their players suspended before the South American Tournament started. Players like Jonathan Dos Santos, Javier Cortes and Marco Fabian are still suspended for four more months. For Corona, this is an opportunity for him to stand out in a squad that is already filled with talent. While those eight players are out, Corona has a chance to show Mexico what he has and Mexico has a need for his talent.
US scouts have sucked, even in finding talent within the US.
Mexico is ahead but don't forget that they still have 8 players (to include Marco Fabian, Javier Cortes, Jonathan Dos santos) suspended for four more months because of their actions before the Copa America. So they don't have it all together.
People should probably stop dissing Gomez, period. He's been arguing for a while that MLS and USNT need to target the dual nationals. If anything, he should get called into camp just because Klinsmann might get better information from him than any scout, just because he actually plays in the league.
Also, people really need to get a grip on the Mexico hate. The rivalry is going to involve a lot of players making choices between sides and we'd just better get used to it.
Coach Paco
Mexico has more players like Corona or better, the only difference here is that he has triple nationality: US, Mexican, and Salvadoreno. Would he elicit so much concern if he decided to play for El Salvador?


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April Stewart
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April Stewart (born February 8, 1968) is an American voice actress. She is well known for voicing mostly all the feminine characters in the "Comedy Central" TV Show, South Park. She provided additional voices for "Cranius Maximus" and Phineas and Ferb: Across the 2nd Dimension.


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TripAdvisor Reviews Caribeno Santo Domingo
Travel Blogs from Santo Domingo
... to find some trousers and treated myself to some coconut body scrub. Bought some food for lunch and dinner at a massive Jumbo supermarket. On the way back, I took the metro and then a concho. The shared taxis manage to fit two people in the front and 4 people in the back all cramped in! In the evening I had dinner on the roof terrace of the hotel and read my ...
Best Project: Courts For Kids
... how little happens on social media in a week- I will drink water out of my tap with joy- I will travel more consciously- I will try to smile as bright as those children at least once a day- I will let things lie more often- I will remember how small the world is- I will keep ...
Waaaay over do blog
... could attend the English course I had started outside of the school that was open to everyone.
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2 English classes: 1 for those in the morning, 1 for those in the afternoon
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Living it up
... br> When I got home from that adventure, my do�a and Mari asked me if I'd like to go to the store with them (note- I was never invites anywhere with my spanish family in all the 4 months I was there). So of course I accepted. We went to a mall that was on the outskirts of the city, but comparable to the MOA. not nearly that huge, but that's the feeling it gives. I didn't get anything, but Mari bought me some cotton candy.
Visit to la Zona Colonial
... in party mode, we decided to have a couple of drinks on the rooftop terrace of the hotel - which was lovely and breezy in the evening.
The next morning, I was very happy to have an amazing breakfast, with fruit, coffee, fresh bread, and a hot plate to order...and CHOCOLATE CREPES were on the menu - any guess as to what I ordered?! For me, the crepes were the perfect way to end a perfect visit to the Zona - I can't wait to go back again sometime soon! ...
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Casa Naemie11 Calle Isabel la Catolica, Santo Domingo | Hotelfrom $82


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July 21, 1985
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Sunday, July 21, 1985
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Friday, July 19, 1985
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Tuesday, July 23, 1985
New Braunfels Herald Zeitung
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New Braunfels Herald Zeitung
New Braunfels, Texas
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New Braunfels Herald Zeitung (Newspaper) - July 21, 1985, New Braunfels, Texas 4A New Braunfels Heiald Zeitung Sunday, July 21,1985 I' Owners Remove Ct ii * I DHS Marble Ii I)cl! U5 j f I Slot Machines ( X I <] __ --- I in (-J Numerous lot 'Ill ]—!. ens were Sp I .ii.- f i in I ii ic* it neee- shiv to k IMW I j folios of .•incise lieut ti ii-', week os I 11;«-y f ti !*•«! lo in I slot loocliiie's aud o pinions Hvrald-Zeitung Dave Kramer, Editor and General Manager Susan Haire, Managing Editor tlllll|!|l(||i(llll|l LOEFFLER EVES THE GOVERKlER'S SEAT... Guest viewpoint IRS deficiencies may open some eyes in Congress James Kilpatrick Ins and outs of English language usage I^ast month Newsweek magazine carried a piece about changing systems of health care. Many alternatives, said the writer, are now available. "In cities like San Francisco and Minneapolis, for example, roughly a third of the population is now enrolled in HMOS " Aaargh! lf the case will now subside, we will hear the semiannual lecture on the issue of "like'' when "such as" is intended. Go back to the offending sentence. What does it tell us about HMOs > Health Maintenance Organizations I in San Francisco and Minneapolis? It tells us nothing at all about HMOs rn San Francisco and Minneapolis. It tells us that iii cities LIKE San Francisco and Minneapolis — San Diego and St. Paul, perhaps'* Roughly a third of the population is now enrolled, etc. And this is not at all what the writer intended The misused "like" and the misplaced "only," which is the subject of lectures in spring and fall. are among the most common errors in everyday composition. I am full) aware that both Bernstein and Follet. two ordinarily infallible authorities, sniffed at the whole business of "like" and "such as." They scorned my position as "specious, but they were quite simply wrong Of course there are tunes when like' is proper: "laves like Lincoln's inspired us all." But when our purpose is to illustrate by way of specific example, we want "cities such as Minneapolis and San Francisco." As a matter of fact, in the sentence under examination, what we wanted was neither "like" nor "such as." What we wanted was "In San Francisco and Minneapolis, for exampl, roughly." To less contentious matters: The dear old Associated Press, God bless it, continue to have its problems with spelling and syntax. From the AP in Nashville, we learn that a group has been organized by President Reagan "to help get Fisk University back on its feat." ... From the AP in San Fransisco, in s story about a pet timber wolf named Sylvia, we get this report: Authorities had been concerned that Sylvia ought harm someone, w ho had been kept as a pet since she was 2 weeks old. if cornered " ... From the AP in West Point, California, we learn about the maniac leonard take: "He found newspaper ads. some of whom he allegedly set loose in his cabin and hunted down like animals." ... And from the AP in Epsom. England, we are advised that "Steve Cauthen became the first U S. jockey to win the 206th Epsom Derby in 79 years." In Northern California, a monthly magazine called In Flight reminded its readers that "the material in this and every issue is copy written." Across the country, in Roanke, Virginia, a cartoonist named Jack Young inserted a classified ad in the World-News He wanted everyone to know that his creations, Skipper, Herbie, and Johnny the Worm, "are copy written." Let us now write IO times upon the blackboard, "copyrighted." Gripes, peeves and crotchets: Jean McCracken of Portland, Oregon, winces when "forte" is pronunced "for-tay." ... Doris Levine, also of Portland, is annoyed by either side" when "each side" is intended. ... Mary Baresh of Seattle objects to starting sentences with "and" or "but." ... Keith tweeds of Indianapolis is fed up with "chairperson," "waitperson" and the like. ... Sid Morris of Miami niminates as an especially stupid phrase "to have sex." Homer Brown of Cary, North Carolina, takes the week's prize for lining up 14 crochets all in a row. Among them: window of opportunity, fine-tune, hands-on, grass roots, domino effect, safety net. leg up, cutting edge, bottom line, and bite the bullet. He writes that he's 76 and has been compiling a notebook of nuisances for years. These, he says, are "just the tip of the ice berg." By TOM LOEFFLER U S Representative During my six years as representative of the 21st District in Congress, I have never received more complaints and inquiries about the Internal Revenue Service than I ave during the past four months. The IRS has always been the federal agency Americans love to hate. During the 1985 tax season, however, passive dislike for this necessary institution has turned to rage and distrust as Americans have reacted to reports of shredded IRS documents in Austin and massive backups in Philadelphia My friend and fellow Texan. J J Pickle, has taken die lead in investigating die discrepencies uncovered in the HLS Service Centers, with particular emphasis on the paper shredding incident in Austin According to HLS investigators, a first-line unit' supervisor at the Austin service Center ordered an employee to destroy between 5.00 and 6.OIH) pieces of taxpayer corespon-dense Luckily, no actual tax returns were destroyed, and the IRS lias taken steps to correct the procedures which had been used in the Austin • enter The Philadelphia Service Center, which had computer and human problems even more severe than the rest of the country this year, will not wrap up processing of all its cases until August More than 176.000 pieces of taxpaper correspondence have piled up at the Philadelphia Center and, to date, remain unanswered In response to questions from tin* members of the Ways and Means Oversight Subcommittee. IRS Commissioner Roscoe Egger testified that as of June I. more than a Mullion taxpayers who had filed their returns on time had not yet received refunds to which they were entitled. Given the fact that th*-average refund this year is $833, one can calculate that as of June I. th* IRS was withholding more than $"!• million which was due American taxpayers. The federal government pays a high price for these late returns, toe By law. the government must pay I percent interest on all refunds net returned to taxpayers by June I Commissioner Egger s promise that virtually all outstanding returns would be processed by late July die little to allay the concerns of th* members of the Ways and Meal committee Clearly, the Internal Revenue Service is in need of a thomug! housecleaning This agency has not been responsive to the public, ha broadened its powers unecessartly and is becoming increasingly mef Relent The growing inability of the IRS to carry out its basic tax collecting and processing tasks is only one of the very real reasons that Americans have lx*en in re * tit years, the Congress has tried wit! little or no success to impose artier and enforce equity at the IRS Perhaps the glaring inefficiencies and deficient es which •* culled during the 1985 lax season will at last open Congress eyes and stiffen otr spine in dealing with this pow el ti federal bureaucracy Remember when 10 years ago. With the filing deadline for Places 1.2 and 3 on the City Council less than a month away, Mrs. C.F. (Margaret! Naegelin and W.H. "Bill" Brown, the current mayor, have both announced their candidacy. New Braunfels Schuetzenverein marksman Bill Wimberley claimed the Simon Memorial Medal Sunday afternoon at the club range by outscoring 29 other shooters with a score of 247. 25 years ago Bids for construction of the main embankment of Canyon Dam will be opened next Tuesday in the new South Texas resident office of the Corps of Engineers at Canyon Dam site, instead of the Fort Worth District office, it was announced last week. Mrs. Anna Lee Meek, 2045 Eastman, who will join the New Braunfels IndepeFort Worth District office, it was announced last week. Mrs. Anna Lee Meek, 2045 Eastman, who will join the New Braunfels 50 years ago. Sheriff August Kne. eh, his son, Othmar Knetsch, and I uty Sheriff John Marion made a trip to Chickasha, Oklahoma, this week to secure two prisoners held there on charges of stealing a car belonging to Department leaders have been named for the Comal Independent School District middle and high schools. Canyon High leaders will be Mrs. Thomas (Carter! Casteel. social studies; Mrs. Robert (Audy > L'Roy, English; Mrs. James (Marian! Richardson, math; and David Gay re, science U.S. Rep. Bob Krueger and congressional dignitaries toured the NASA headquarters recently in Houston where they were briefed on the Apolto-Soyuz space flight by astronaut Vance Brand and Donald Slayton. The cities of New Braunfels and Schertz made moves to reconcile their boundary dispute when their legal reprsentatives agreed in principle last week that New Braunfels should extend its southwestern boundary to the present limits of New Braunfels' ex traterritorial jurisdiction Attorney Steer Taylor addressed Hie Mission Valley Home Demon stratum Club on July I with a talk on estate planning and keeping wills up to date (Tinton Die ie rf. a 1973 graduate of Canyon High School, was named the winner in the crop production contest of the 47th annual Stale Future Fanners of America Convention held in Houston last w eek Independent School District staff as a special education teacher this fall, is one of 17 teachers attending a cerebral palsy workshop at the University (rf Texas The $104,475 grant to the New Braunfels Independent School District to aid in building a new high school, which appeared to have been lost because of the defeat of the proposed school building program bond issue, will be made any how , Cecil Wills, school superintendent,™ Stables into eight classrooms was granted to Emil Seidel on his low bid of $9,978 by the New Braunfels Independent School Hoard at its meeting 'I uesday night Barbara Belize and F.rnestiue Sisal* both students at Canyon High School, will participate iii the fourth annual Cheerleader Clinic Aug 8-12 at Lamar Tech. Mrs. Merritt Schumann entertained with a coffee Thursday morning, July 14, in lier home, honoring Mrs. Mary Leibu of Wilmington, Delaware, who is currently visiting here with her parents Mr. aud Mrs. K E. Tun ntermauu Descendants of Mr. Wilhelm Atwood his wife, the former Dora Stein met at Gruelled Hall Sunday af ternoon and evening, July 3. for thei first family reunion Following ai i apian a af a $. if check from the San Marcos Chambe of Commerce the Slate Parks Boar Monday announced that it will nam the proposed 400 to 500 acre stat park on the northeast shore < Canyon Dam Reservoir San Marcc State Park " Mrs. Meta Schmidt here Friday night Dietrich Hoffmann, 70, a former resident of Schumannsvilie, sustained severe injuries Monday U s & x V G § a AU I'M SAYING, HAILE, IS THAT YOUR GOVERNMFNI HAS TO (DOP erne mope in the ruff tt-fon, I OR THERE'LL BE A PEAL .......... n PU0UC BACKLASH IN THE MEST IF YOU REEP USING FAMINE AS A WEAPON IN YOUR CML MAR, YOU'RE GOING TO FI NP YOURSELVES TOTAL LYPEPENPFNT ON IHF ROMEIS,MHO CANT even FEW THEIR eT OMN PEOPLE, IST ALONE \ 10UPS' ■" morning when the car in which he was riding was struck by a Missouri Kansas and Texas railway train at a crossing on the old San Antonio highway, about 5 miles south of New Braunfels Representatives of the church board and various organizations of the First Protestant Church met Tuesday night to formulate plans for the celebration of the congregation's ninetieth anniversary, which occurs tins year. A closing program by German summer school students will be given at Echo Hall Friday night, July 19, at 8 o'clock, according to an announcement by Curt Schmidt, principal of the school. Numerous local persons were finding it necessary to seek ne forms of amusement this week i they failed to find slot machines ai marble boards in their usual haunt The devices were noticably aba# about town since Monday, ti deadline set by Sheriff Augu Knetsch in Ins drive to rid the coun of this kind of vice After several months of discuss! with dog owners, the Board of CT Commissioners Monday secur passage of a revised dog ordman providing for the licensing ai keeping of dogs within the city. Acting with the recoiiunendation the Board of City Development, el officials this week inai preparations to close the purchase a strip (rf land adjoining the cl water works in a new developmf known as luanda Park Highlands. ;
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text:
The Cat and the Canary
- Where: Roleystone Theatre 587 Brookton Highway, Roleystone, WA 6111 AU
27th May, 2016, 8:00 PM - 4th June, 2016, 10:30 PM
add to calendar
- Event Type: Arts
- Phone: 9490 9306
- Email: email@example.com
- Website: http://www.roleystonetheatre.com.au
- Connect:
A spooky haunted mansion, possible heirs to a fortune and a variety of secrets and mysteries are the key ingredients of Roleystone Theatre's latest production.
Written by John Willard and directed by Paul Treasure, The Cat and the Canary is a 1922 comedy thriller that sees a group of distant relatives gather at a creepy old house to find out who will receive the inheritance of an eccentric millionaire, 20 years after his death. The heir is then forced to remain in the house to prove she's not mad – but, at the same time, a homicidal maniac has escaped from a nearby asylum. There is also a lost emerald necklace, secret panels and a mysterious housekeeper in contact with the spirits.
The Cat and the Canary plays at 8pm, May 27, 28, June 1, 3 and 4. Tickets are $20, $15 concession – book on 9490 9306 or at www.roleystonetheatre.com.au


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Thank you so much. As 2011 drew to a close, you stepped up along with thousands of others just like you to support our troops. Your generous donations and support allowed the USO to bring a touch of home to troops stationed around the world.
Because of the generosity of supporters like you, we were able to lift spirits at the more than 57 forward operating bases and combat outposts near Kandahar where our brave men and women are working hard to protect our freedom. But instead of hearing all the good you provided from me, I wanted to share this special message of thanks direct from the front lines in Afghanistan.
Please take a minute to see the faces and hear the words of the brave men and women whose spirits you lifted.
Many of our troops returned from Iraq in 2011, and we are honored by their service. Our commitment to them and their families is ongoing. So is our dedication to the thousands of troops stationed around the world, making incredible sacrifices to protect our freedom.
We couldn't do all that we are able to do for our troops were it not for you. Thank you for everything you do to support our service men and women and to remind them that they are always at home in our hearts. - Sloan Gibson, USO CEO & President
More from the USO
May 25, 2017
NBC's 'This Is Us' Cast Visits Service Members and Military Families on USO Tour at Joint Base McGuire-Dix-Lakehurst
It was a morning to remember. A group of stars from NBC's "This Is Us" – Mandy Moore, Milo Ventimiglia, Chrissy Metz, Justin Hartley, Susan Kelechi Watson and Chris Sullivan – spent a morning visiting service members and their families at Joint Base McGuire-Dix-Lakehurst as part of a USO entertainment tour.
May 24, 2017
After Struggling to Find His Place in the World, Marine Finds Two Families – and His Place in Both
Deron Johnson didn't plan on joining the military, but he ended up a Marine. He also didn't anticipate becoming a fraternity brother at Texas A&M, but then came Theta Chi and its history of supporting the military - and the USO.
May 15, 2017
USO Operation Birthday Cake Brings the Party to Service Members in Japan
When Navy Petty Officer 3rd Class Jeffrey Lee received an urgent call to head to his work station one morning, he thought there was something seriously wrong, but there was no emergency. Instead, he walked into his own surprise birthday celebration, courtesy of his wife, USO Yokosuka and the Operation Birthday Cake Program.


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Latest news
- Expedition Report Published March 3, 2017
- Polar Film Fest September 23, 2016
- Outdoor Women's Alliance Post-Expedition Feature September 23, 2016
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Tag Archives: Dating
Highly connected Greenland and central European climate, 30,000-60,000 years ago
A new paper has been published today in Geology by the Northeast Greenland Caves Project leader Dr. Gina Moseley and science partner Prof. Christoph Spötl, which shows that in the cold, glacial climate c. 30,000 to 60,000 years ago, strong teleconnections existed … Continue reading
Posted in Publication, Science Tagged Alps, Cave science, Caves, climate change, Dating, Earth Science, Environment, Europe, Geochronology, Geography, Geology, Gina Moseley, Glacial, Greenland, Greenland Ice Sheet, Outreach, Palaeoclimate, Palaeoclimatology, Physical geography, Pleistocene, Publication, Quaternary, Research, Science, Stable isotopes, U-Th dating Leave a comment
Short cave-science documentary film
Thanks to expedition partner Robbie Shone, and also to Martin Moseley for his editing skills, we now have a short documentary about the work of the Innsbruck Quaternary Research Group in Conturines Cave in the Dolomites. The work in the … Continue reading
Posted in Science, Short films Tagged Alps, Cave bear, Cave science, Caves, Christoph Spoetl, climate change, Conturines Cave, Coring, Dating, Documentary, Dolomites, Earth Science, Environment, Film, Geochronology, Geography, Geology, Gina Moseley, Innsbruck Quaternary Research Group, Italy, Karst, Martin Moseley, Palaeoclimate, Palaeoclimatology, Paleomagnetic dating, Physical geography, Pleistocene, Quaternary, Research, Robbie Shone, Science, short documentary, short-film, Stable isotopes, Surfacing Productions, U-Th dating Leave a comment


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First we had news of Telltale's Back To The Future adventure game. Then, we had pictures. Today, we have… moving pictures. What will come next? Nothing, I think. We're at the top of the tech tree, gentlemen. It can't get any better than this. Watch the eerily expressive eyes of Marty McFly after the break. The break… to the future!
Read the rest of this entry »


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Discussion in 'Draft Zone' started by Woods, Apr 27, 2012.
He's not even in the top 200 rankings accordings to Ourlads . . . .
Anyone familiar with this guy?
I had him at 230
Wow, on the Ticket they are saying the guy was going to be a FA according to many scouts . . . .
I thought that the NE guy input the wrong name in the system . . . . :laugh2:
Seems like a Raiders pick. When they picked that Safety Mitchell I think a couple years ago . . . .
I watched some Illinois (being a Big 10 guy). He was good, but can't say he was anything special.
356 on my board
All the Sirius guys had him as a after the draft FA pickup but because its the PAts no one wanted to criticize the pick.
And I think the Pats only have 1 pick left this entire draft unless they trade it for more picks . . . .
Yeah, given the last decade I kept waiting for some franchise to trade them a ONE next year for one of the pats seconds. Man that would drive me crazy.
Al Davis in witness protection in NE?


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Wildwood, NJ, USA < change city >
Reception Sites (44)
Cakes (13)
Florists (13)
Bands (9)
Wedding Day Beauty (9)
Photographers (9)
DJs (6)
Invitations (5)
Officiants (5)
Wedding Fashion (5)
Caterers (3)
Rentals (3)
Limos (3)
Coordinators (2)
Ceremony Musicians (1)
Photo Booths (1)
Dance Instruction (1)
See All Vendors >
Kimberly and Tyrone's Wedding
"The venue was great as far as location and food. "
Kristina and William's Wedding
Ceremony at Aqua Beach Resort: Morning Glory Rd & Ocean Av
Reception at Beach Creek Oyster Bar & Grill
Wildwood Wedding In October
Ceremony at The Wildwood DooWop Museum
Reception at Wildwood Golf & Country Club
Wildwood Wedding In August
Caleen and Frank's Wedding
Ceremony at Urie's
Reception at Urie's
Wildwood Wedding In December
Ceremony at St. Ann Church
Reception at Avalon Links Restaurant
Stephanie and Chris's Wedding
"This was truly the wedding of my dreams. Every moment exceeded my expec..."
Rose and Joe's Wedding
Ceremony at Abundant Life Assembly of God
Reception at The Hotel Alcott
"My wedding day was everything i could have wished for. Both my husband a..."
Keri and John's Wedding
Reception at The Yacht Club of Stone Harbor
Karen and Michael's Wedding
Ceremony at Cape May Point State Park
Reception at Peter Shields Inn
"Our wedding day was perfect. The beach ceremony was amazing. The surf ..."


text:
Breitling Bentley Motors 6.75 K44362
- Stock #: WP4426
- Brand: Breitling
- Series: Bentley
- Model: K2536212.G555.755P
- Retail Price: $28,500.00
- Please Inquire
Condition: New
Gender: Men's
Dial Color: Silver
Case: 18k Yellow Gold 48mm
Strap: Crocodile Leather
Bezel: 18k Yellow Gold
Crystal: Scratch Resistant Sapphire
Movement: Automatic
Water Resistant: 100 meters / 330 feet
Box: Yes
Papers: Yes
Certificate of Authenticity: Yes
Warranty: Yes
Additional Information:
Brand new Breitling Bentley Motors 6.75 gent's watch, Model # K44362.
18kt yellow gold 48mm case. Sapphire crystal glass. Silver dial with luminous hands and hour markers. 18k yellow gold crown and pushers. Crocodile leather strap with 18k yellow gold buckle. 18k yellow gold bi-directional bezel. 18k yellow gold lugs. Hours, minutes, seconds, big date, slide rule and chronograph. Automatic movement. Waterproof 100 meters / 330 feet. "Slide Rule" aka "Navigation Computer" which allows computing different airborne calculations such as fuel consumption, climbing times and converting miles into nautical miles or kilometers.
Also known as:
Britling, Brietling, Bently, limited, K44362
Subject to Availability


text:
NCI Dictionary of Cancer Terms
The NCI Dictionary of Cancer Terms features 8,107 terms related to cancer and medicine.
- naloxone
- listen (na-LOK-sone)
- A substance that is being studied as a treatment for constipation caused by narcotic medications. It belongs to the family of drugs called narcotic antagonists.


text: AUD/USD update (7th Dec 2010, 12:00) Market Moves Staff in Forex News 7 Dec 2010 0 share Share Tweet Email Share Despite the lacklustre performance of the national cricket team, the Australian dollar pushed up against the US dollar, moving back towards the parity that it reached a month ago. The increased strength of the Australian dollar may be due to some increase in risk appetite arising from the extension of US tax cuts agreed by Congress, which has helped to outweigh the negative effect of the Reserve Bank of Australia's (RBA) decision to hold interest rates steady at 4.5%. The RBA also signalled that rates may remain static for some time to come, with inflation expected to remain contained 'over the next few quarters'. The main driver of the Australian currency is now likely to be the eurozone crisis, as wariness among investors leads to increased risk aversion. RBC commented that the RBA will be 'sidelined until the middle of 2011' as demand for higher-yielding assets is negatively affected by worries about a eurozone break-up. Until moves to a proper resolution of the crisis take shape, the Aussie will be batting on a sticky wicket for some time to come.


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super sixes
Who doesn't love a list? These are our pick of the best events from beginner triathlons to the toughest 10k's.
Set to take the UK triathlon world by storm, this exciting new event, based at the beautiful award-winning Putsborough Sands in North Devon, takes in some of the South West's most stunning scenery. Swim in the Atlantic ocean along Putsborough beach, where the water quality is superb; the bike route offers fantastic coastal views, challenging climbs and steep descents; the run is demanding, hilly and possibly muddy, and has been devised by local athletes to give fabulous ocean views and include a run along the famous Croyde surfing beach.
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Genome-scale modeling using flux ratio constraints to enable metabolic engineering of clostridial metabolism
Synthetic biology
Genome-scale metabolic networks and flux models are an effective platform for linking an organism genotype to its phenotype. However, few modeling approaches offer predictive capabilities to evaluate potential metabolic engineering strategies
A new method called "
FBrAtio is a promising new method for constraining genome-scale models using internal flux ratios. The method was effective for modeling wild-type and engineered strains of
Modeling clostridial metabolism
Butanol is of considerable research interest as a potential biofuel, and its renewable production through fermentation is sought largely from the clostridia. In particular,
Engineering clostridial metabolism
Knowledge of the metabolic pathways of butanol fermentation has allowed for targeted engineering approaches. As acids (acetate and butyrate) and alcohols (ethanol and butanol) are competing products of fermentation, metabolic engineering strategies have been designed to silence acid producing pathways in attempt to re-direct carbon flow into the alcohol producing pathways. The primary metabolic network of
Figure 1
Primary central carbon metabolism of
Primary central carbon metabolism of
Metabolic engineering
The goal of metabolic engineering
With the ultimate goal of re-directing metabolic flux through the butanol production pathway in
Considering metabolic flux ratios
The experimental determination of metabolic flux and pathway usage through the use of isotope tracers has significantly contributed to the overall understanding of regulated metabolism. One approach to characterize metabolism is through the use of metabolic flux ratio analysis (METAFoR)
Genome-scale modeling with flux ratios
Of the several successful (and unsuccessful) metabolic engineering strategies applied to clostridia (many of which are not mentioned here), it was not immediately apparent which design(s) would be successful upon conception. The mutant strains had to be created in the laboratory and analyzed. From these results, hypotheses were formed that guided more advanced designs. The purpose of metabolic engineering
Genome-scale model
A new genome-scale model for
Additional file 1
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Additional file 2
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Flux balance analysis
The specific proton flux
The concept of the specific proton flux (SPF) was first introduced by Senger and Papoutsakis
FBrAtio algorithm
Metabolic engineering
Next, a ratio of fluxes for these reactions is assumed. For this hypothetical example, it is assumed that twice as much flux proceeds through the THL reaction (Equation 1) than the PTA reaction (Equation 2). Ultimately, biochemical origins of differing fluxes through competing reactions, where known, can be used to calculate flux ratios. The flux through the reaction catalyzed by THL is
To build this flux ratio constraint into the stoichiometric matrix (
Next, a new row is added to the stoichiometric matrix. In this new row, two values are added (all other values in the row are zero). In the column representing the reaction catalyzed by THL (Equation 1), the coefficient 1 is added to the matrix. In the column representing the reaction catalyzed by the PTA, the coefficient −2 is added (in the new row). With these additions, when the flux balance equation (
Simulations performed
The goal of this research was to develop a method of constraining a metabolic network so that metabolic engineering can be performed
Simulations with a minimal set of constraints
Approximating wild-type metabolism with FBrAtio
Obvious futile cycles allowing artificial NAD(P)+/NAD(P)H balancing were located and corrected, but the problems described above remained. Given the ability of the metabolic network to artificially balance NAD(P)+/NAD(P)H without using the acid/solvent production pathways, additional constraints and flux ratio constraints were implemented. First, the reactions involving the ferredoxins were further constrained for irreversibility. These reactions and their updated constraints are given in Table
Figure 2
FBA results of wild-type metabolism using
FBA results of wild-type metabolism using
A total of five flux ratio constraints were found necessary to generate simulations of metabolism that were qualitatively consistent with experimental observations. First, the butyrate to acetate uptake ratio was constrained according to previously published findings
Implementing this flux ratio alone led to high initial production of succinate, and hydrogen production increased during solventogenesis. Over-production of ethanol and lactate was also observed. So, a flux ratio was installed to direct the conversion of pyruvate to either lactate, through the lactate dehydrogenase (LDH), or to acetyl-CoA through the pyruvate ferredoxin oxidoreductase (PFO) (see Table
Simulations with these two flux ratios were characterized by (i) high acetate and ethanol production, (ii) low butyrate and butanol production, (iii) high initial secretion of succinate, and (iv) low initial production of hydrogen. The secretion of succinate was investigated next. This is indicative of high fluxes through the TCA cycle. No significant succinate export has been reported for
This flux ratio constraint corrected hydrogen production (i.e., high hydrogen production during acidogenesis and reduced during solventogenesis) and minimized succinate secretion, which is consistent with experimental observations. This constraint also resulted in increased CO2 production during solventogenesis, relative to production during acidogenesis. However, metabolic activity was still characterized by (i) high acetate and ethanol production and (ii) low butyrate and butanol production (results not shown). To address this, flux ratios were constructed around the use of the acetyl-CoA metabolite pool in clostridial metabolism (see Figure
Figure 3
FBrAtio results of wild-type metabolism using
FBrAtio results of wild-type metabolism using
asRNA knockdown of CoAT only
Previous research found that knockdown of the CoA transferase using asRNA technology resulted in a more favorable butanol to acetone selectivity. Although, overall reduced butanol yields were observed
Figure 4
The wild-type
The wild-type
FBrAtio results with the
Figure 5
FBrAtio predictions of solvent production using the
FBrAtio predictions of solvent production using the
It was important to determine whether the
Figure 6
FBrAtio predictions of solvent production using the
FBrAtio predictions of solvent production using the
Knockdown of both CoAT and AAD
In published research
Figure 7
FBrAtio predictions using the
FBrAtio predictions using the
Over-express AAD and knockdown CoAT
The AAD was over-expressed using the
Figure 8
FBrAtio predictions using the
FBrAtio predictions using the
The large number of degrees of freedom in primary clostridial metabolism makes this system challenging to model. Initial efforts
The use of metabolic flux ratio constraints through FBrAtio enabled qualitatively accurate modeling of acidogenic and solventogenic metabolism of
FBrAtio is a new method to derive metabolic engineering strategies to achieve optimum phenotypes. The concept of using metabolic flux ratios was initially developed with the METAFoR approach
The FBrAtio approach is presented in detail here and is applied to model previously published metabolic engineering approaches in
The FBrAtio approach for -incorporating metabolic flux ratio constraints into a genome-scale metabolic network and generating solutions using simple linear programming was developed in this research. The approach proved effective in modeling wild-type metabolism of
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
MM is responsible for constructing the new genome-scale model of
This research was funded by the USDA AFRI Biobased Products and Bioenergy Production Program (Award Number 2010-65504-20346). BF was supported by the Doctoral Scholars Program of the Institute of Critical Technologies and Applied Science (ICTAS) at Virginia Tech. JY was supported by a grant from the Biodesign and Bioprocessing Research Center at Virginia Tech.
Equations and calculations for fermentations of butyric acid bacteria
Acetone and butanol production by
Regulation of
Stoichiometric modeling of
Bioreaction network flux analysis for industrial microorganisms: A review
Metabolic engineering of the non-sporulating, non-solventogenic
Metabolic flux analysis elucidates the importance of the acid-formation pathways in regulating solvent production by
Stoichiometric modeling of
Genome-scale reconstruction and in silico analysis of the
Biofuel production improvement with genome-scale models: The role of cell composition
Genome-scale model for
Genome-scale model for
Accomplishments in genome-scale in silico modeling for industrial and medical biotechnology
The growing scope of applications of genome-scale metabolic reconstructions using
Reconstruction of biochemical networks in microorganisms
Metabolic pathways in the post-genome era
Comparison of network-based pathway analysis methods
Genome-scale microbial
Genome-scale models of microbial cells: evaluating the consequences of constraints
Towards multidimensional genome annotation
Analysis of growth of
Reconstruction, modeling & analysis of
Matrix formalism to describe functional states of transcriptional regulatory systems
Probabilistic integrative modeling of genome-scale metabolic and regulatory networks in
An expanded genome-scale model of
The genome sequence of
Targeting multiple targets in
Genome-scale metabolic network analysis and drug targeting of multi-drug resistant pathogen
Genome scale reconstruction of a Salmonella metabolic model: comparison of similarity and differences with a commensal
The genomic and epidemiological dynamics of human influenza A virus
In silico genome-scale reconstruction and validation of the
Metabolic functions of duplicate genes in
Optknock: a bilevel programming framework for identifying gene knockout strategies for microbial strain optimization
OptStrain: a computational framework for redesign of microbial production systems
OptForce: an optimization procedure for identifying all genetic manipulations leading to targeted overproductions
The transcriptional program underlying the physiology of clostridial sporulation
In vivo methylation in
Genetic manipulation of acid formation pathways by gene inactivation in
Characterization of recombinant strains of the
antisense RNA downregulation of coenzyme a transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic
Aldehyde–alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in
Metabolic flux profiling of
High-throughput metabolic flux analysis based on gas chromatography–mass spectrometry derived 13 C constraints
Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism
NADH availability limits asymmetric biocatalytic epoxidation in a growing recombinant
Metabolic fluxes in riboflavin-producing
Design of antisense RNA constructs for downregulation of the acetone formation pathway of
KEGG: kyoto encyclopedia of genes and genomes
Resolving the TCA cycle and pentose-phosphate pathway of
Systems-level metabolic flux profiling elucidates a complete, bifurcated tricarboxylic acid cycle in
Group contribution method for thermodynamic analysis of complex metabolic networks
Thermodynamics-based metabolic flux analysis
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox
In silico predictions of
Fermentation characterization and flux analysis of recombinant strains of
Increased levels of ATP and NADH are associated with increased solvent production in continuous cultures of


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A:I'm happy to help, CJ! You will not have to remove your catalytic converter, these Magnaflow cat-back systems are designed to be mostly bolt-on installation. It would be illegal for your catalytic converter to be removed if you are still driving on the road. The part that you're speaking of is a resonator. If you would like to take a look the installation instructions are located here: http://www.magnaflow.com/07techtips/pdfs/16731.pdf


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To most people, water generally tastes the same, well unless it is cold, hot or flavored. All that perception will change when you try Waiakea Hawaiian Volcanic Water which was launched in 2012. Since its inception, this water has been loved and embraced by foodies all over the world for its unique taste and terroir. You can enjoy this water with various foods. From sushi to the traditional Hawaiian poi, Waiakea compliments almost every dish. Learn more about Waiakea Water: http://www.ebeverageservice.com/waiakea-hawaiian-volcanic-water-500-ml-12-ct/
The terroir and taste are determined by its virginality, minerality, orientation and vintage. The number of minerals disintegrated in water is measured as tds [total dissolved solids]. Particular water's tds consists of minerals such as Sodium, Potassium, Silica, Magnesium, Calcium, Iron among others. Waiakea water contains a tds of 79. This gives it a light, crispy taste that, unlike other remarkable tds mineral waters, can get paired with almost all kinds of food.This specific combination of minerals; particularly the presence of silica gives Waiakea water its mouth feel silky smooth taste.
Virginality refers to how well water is protected from its surroundings. This is determined by the level of nitrate in the water. Nitrate can easily be carried by water through the soil, and this contamination may originate from animal waste products, septic tanks, fertilizer, decaying plant matter, etc.
Waiakea has a unique volcanic filtration process that keeps the harmful contaminants away, giving this water a superior virginality rating with its nitrate level being a mere .59 mg/l. The low nitrate level is also due to its vintage. Vintage describes the age of water before being sourced.
Waiakea is young and has a constant flow. It takes it a maximum of 30 days to reach its destination from its origin at the Waiakea spring. Young waters like Waiakea do not have that much time to absorb many contaminants or minerals as mentioned above, and this lends it a crispy, light and clean taste.
The packaging of the bottle is plastic, but it has an attractive square kind of shape. On this bottle, it is indicated when the water was packaged as well as when it's best by.
According to Specialty Foods, With its naturally enhanced minerals and electrolytes, Waiakea water offers a refreshing taste. It is not your ordinary boring water. This is the freshest water you will ever taste. It is an excellent additive that is not only tasty but comes with health benefits as well. Who would have thought that all these could be found in just water.


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If your guy is "the man" when it comes to gambling, the casino king personalized Christmas ornament is perfect. These large resin Las Vegas ornaments personalized by our lettering artists come with a custom message on the back. Let us know what you want the casino king Christmas ornament to say, and we'll make sure it becomes a lasting keepsake he'll enjoy every year. It arrives in a handsome velvet gift pouch with our display cord attached for hanging on the tree, in the man cave or even his car.
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Usability review: Who designed this thing anyway?
by Scott Rains
The Rolling Rains Report
Ever find yourself asking, "Who designed this thing anyway?" I do. I also found someone who figured out what to do next. Her story might someday impact us all.
Usability, accessibility and universal design
Julie Jones is founder and CEO of Engineered Travel, a company that works with manufacturers of existing products and shows them how to apply Universal Design thinking. The result can be anything from product improvements involving simple engineering changes to the development of entirely new products to meet the needs of the disability market.
Julie has recently teamed up with Bob Davis of the GEOS Travel Safety Group who provides integrated security, safety and resilient communications services for corporations as well as domestic and international travelers. With former police, military, and government security professionals in-house and having extensive knowledge of search and rescue (SAR) practice GEOS partnered with the manufacturers of the SPOT Satellite Messenger to provide a new type of personal safety device.
I have been field testing it.
I like it!
Let me reiterate that the purpose of Engineered Travel is to take products that were not designed with the disability community in mind and make them accessible. That said, even with opportunities for design improvements I have had fun playing with SPOT as I traveled from Brazil to Alaska. It evoked a satisfying round of "tech envy" as I took it out of my briefcase during a meeting of technology innovators in San Francisco recently. I am getting quite a few requests to be added to the list that receives a SPOT "Check In" email pinpointing me on a Google map as I globe trot with SPOT.
There is always inconvenience, even risk involved in travel. For someone with a disability what may be inconvenience for some can be a risk - a vehicle that goes dead somewhere out of cell phone range for example. This is where a personal location device like SPOT literally becomes a life saver.
The orange and black ruggedized waterproof unit has several functions. These include 9-1-1 mode, a less urgent Help mode, and a simple Check-in mode.
From the SPOT website here is how 9-1-1 mode works:
Once activated, SPOT will acquire its exact coordinates from the GPS network, and send that location along with a distress message to a GEOS International Emergency Response Center every five minutes until canceled. The Emergency Response Center notifies the appropriate emergency responders based on your location and personal information - which may include local police, highway patrol, the Coast Guard, our country's embassy or consulate, or other emergency response or search and rescue teams - as well as notifying your emergency contact person(s) about the receipt of a distress signal.
In Help or Check-in mode SPOT contacts those who you have designated to receive an SMS message or an email. The email also includes a link to a Google map showing your location within 15 feet. At your SPOT account online you compose the email and select who you want to receive the message before you travel.
Something that seems ingenious is the SPOT Tracking feature. It is the second function to the OK button used for Check-in mode. This "Optional feature" is very useful in the event you need to be located but also need to move. Tracking leaves a breadcrumb trail of where you have been. Once activated this feature plots your location every 10 minutes for 24 hours, without having to re-push a button.
SPOT distinguishes itself from existing products in a number of ways. It uses satellite technology and the GEOS Emergency Response Center that is not dependent on cell phone reception, Electronic Position Indicating Radio Beacon (EPIRB) or the PSAP (Public Service Access Points) network. This adds a layer of human contact which, in one case described to me by Bob Davis of GEOS, led to additional lives being saved.
I was told one story about a rescue using SPOT. In an avalanche situation a SPOT owner's distress call was verified by GEOS. (Note: SPOT is not designed to function under snow.) GEOS call to the owner's emergency contact revealed that the owner's party included eight people. That information allowed the Search and Rescue team to be prepared and make a successful rescue.
SPOT is waterproof although it is not designed to signal effectively when submerged in water.
This feature, that SPOT is waterproof for up to 30 minutes even when submerged at 1 meter, was quite a reassurance as I kayaked through an ice flow in Alaska last month with the unit in my pocket. Doubly reassuring as one iceberg chose to imitate a rotary saw blade by flipping top for bottom just after we passed it.
More often I suspect that the waterproofing feature will come in handy when I am juggling coffee cups or at the pool.
In my analysis of the product, and the team behind it, my opinion is that the three part team involving SPOT, GEOS, and Engineered Travel is what makes this product/service bundle such a potential benefit to the disability community. Team members express genuine interest in achieving usability for, to quote the classic definition of Universal Design, "the widest range of people operating in the widest range of situations without special or separate design."
For some people with disability the current SPOT form factor will work just fine. For others Engineered Travel needs to work its magic. In its present form SPOT controls lack sensory redundancy for those of varying abilities.
I found the buttons took effort to push under warm conditions and required me to use a pen or other implement to activate when my fingers became weaker in the cold. The size and non-slip material along the edges were a benefit in gripping the unit but the protruding belt clip on the back makes the unit unstable when laid on a flat surface in order to work the buttons. The easy release fasteners made opening the case for battery insertion possible. The color makes it easy to locate.
Whether you need a safety device for when you are in a cell phone dead zone, a monsoon downpour, or just want to accurately geo-tag your photos on Google maps this is a handy unit to have. Thinking ahead to travel, emergency, or disaster situations where normal communication channels are unavailable I am reminded of the Bonn Declaration that "Disasters are Always Inclusive." This unit could also be a lifesaver.
SPOT is useful in its current version for those with good to fair visual acuity and fine motor skills. Given my conversations with representatives of the GEOS Travel Safety Group and Engineered Travel I suspect that we will see modifications. These in turn will show concretely how the consumer power of the disability community is coming of age and once again demonstrate the Curb Cut Effect of Universal Design to the benefit of us all.
Author disclosure statement
The following article is an extract from a post on Rolling Rains, a popular blog "precipitating dialogue on travel, disability, and universal design" titled "Usability Review: The SPOT Personal Safety Device". As a result of meeting Julie Jones, Scott has since joined Engineered Travel's Disabled Advisors Board. In that role he field tests products such as SPOT. Items are loaned to him for the trial period but he receives no financial compensation or free products and provides design, usability, and market analysis to Engineered Travel - as well as early product reviews for readers of the Rolling Rains Report.
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Granddaughter of Dr. Mary McLeod Bethune leads campaign for the legacy of her memory
Dr. Bethune's wove several insightful stories of her heritage. One of these stories focused on her great grandfather who, as a slave, managed to convince his owner to purchase the woman he loved and eventually married. The quiet persistence and resoluteness in spirit had an effect on her grandmother who also very quietly made a significant difference in our modern society.
She was a close friend of Eleanor Roosevelt and together they tackle issues such as the integration of the American Red Cross and the formation of the Tuskegee Airmen. Dr. Mary McLeod Bethune developed institutions for higher learning, Bethune-Cookman University, worked for world peace (sat with the United Nations founding body) and founded organizations such as the National Council of Negro Women to organize and train women in leadership and planning strategies to improve their communities across the country.
Dr. Evelyn Bethune noted that more than anything else, the legacy of her grandmother was in education. "Education was the first step in her remarkable journey" she stated. She went to say that "education equates to freedom."
In the past 3 years, Dr. Bethune has been organizing a signature event to celebrate her grandmother'
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We operate on the spectacular Otago Central Rail Trail, Roxburgh Gorge and Clutha Gold Trails.
Whether you're after bike hire, bag transfers, transport, an itinerary or some advice - give us a call or email us. Our friendly team is only too willing to help with information or a no obligation quote. Being locals, we know the area inside and out!
Our team has helped thousands of travellers put together great itineraries tailored to their special interests, budgets and time constraints.
With depots at both ends of the Otago Central Rail Trail (Clyde and Middlemarch) this enables us to offer plenty of options to suit your requirements, budgets and preferences.
Otago Central Rail Trail Voted NZ's Favourite Ride
So exciting to see the Otago Central Rail Trail take out the top honour of NZ's Favourite Place to Cycle on March 1st 2016. Thanks to everyone that voted. If you have not experienced this trail for yourself then maybe it is time to put it on your to do list and come and see what all the fuss is about.
To view a small video clip of the Roxburgh Gorge Cycle Trail in Autumn CLICK HERE
The Rural Women Triple F Rail Trail Challenge is coming up again in April 2017.
Trail Journeys are proud to support this Challenge.
We offer a range of bikes and bike accessories for hire:
Trail Journeys Comfort bikes, mountain bikes (for more experienced riders), children's bikes, tow behind bikes, child seats and tandems.