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Justin Bieber pissed off the entire Philippines
Justin Bieber pissed off the entire Philippines after taking to Instagram to mock Manny Pacquiao's recent loss to Juan Manuel Marquez, and now they're threatening to ban him from the country. From Business Insider:
Indignation over Bieber's insult reached the Philippines Congress, with seven law-makers filing a resolution demanding an apology from the singer for the "distasteful comments … lest he be declared persona non grata".
"Mr. Bieber should have known that the Hon. Pacquiao has reached his stature as such as a result of sheer hard work, determination and firm belief in God, and despite such stature best exemplifies humility and grace even in defeat," the congressmen said.
They added that it was not only Pacquiao who Bieber insulted, but also the entire population. Pacquiao released a statement saying he would pray for Bieber.
Am I supposed to believe that Justin Beiber is some sort of tough guy or something? Obviously neither I nor anyone else believes that Justin is a man's man who can handle his business with his fists, and that includes Justin herself. Good God, if Justin was any less intimidating he'd be a member of Fall Out Boy. I know, I know, Justin has those big Twitter muscles that give him a loud mouth, but Manny Paquiao is a real man. Calling out someone's shitty music is just a matter of opinion — calling a man a pussy for losing a fight with a legitimate fighter is what little bitches do. I'd like to see what Justin tweets after stepping into the ring with Pacquiao. Something tells me that there would be a lot less smarminess and a lot more asking for his day nurse to empty his colostomy bag.


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|Property type
|Minimum Stay
|4 nights
Welcome to Bambooshay, St. John!
"Bambooshay" is a Creole word that means "enjoy yourself; have a good time." Coined during Carnival season in the 1950's, the word originates from "Bambula," an old African dance that is carried out today through a unique "second line" dance that is performed to the beat of traditional New Orleans jazz. Revelers during carnival today are often heard yelling out "Bambooshay!" as they partake in the festivities of the season.
In a single word, Bambooshay embodies the spirit and attitude that we have found from the Caribbean to New Orleans, and we truly hope you will enjoy yourself and have a great time on St. John. Bambooshay!
Guests arrive at Cruz Bay, where the ferry docks, and will need to rent a car to continue on to the privacy of the villa.
Two roads beckon. The North Shore Road, which winds past Trunk Bay and many other tropical beaches that are ideal for snorkeling and swimming, and Centerline Road, which winds along the highlands, offering vistas of Coral Bay and the British Virgin Islands.
Built for two, the villa has one bedroom (and a self-inflating mattress for an additional visitor), a living room and a modern bath. The kitchen is equipped with a gas range, BBQ grill, refrigerator, dishwasher, coffee maker, toaster and blender and is stocked with pots and pans. Ceiling fans and French doors keep the interior cool.
Perhaps best of all, the villa has an expansive deck with an outdoor shower, and is great for al fresco dining, sunbathing, star gazing or getting a massage. A big hammock for two provides a good place for napping, reading or enjoying the view.
If you feel more like exploring, take an easy 8-minute walk down Seagrape Hill to enjoy the restaurants and shops of Coral Bay. Hike along the nearby Johnny Horn Trail or visit the Moravian church at the foot of Seagrape Hill. The St. John National Park's beaches are just a short drive away.
Book your trip today. St. John awaits you.
Keywords: villa, ocean view, hot tub, upscale, paved, house, water, hammock
We looked all over the Caribbean and ended up choosing St. John for many reasons: pristine beaches, 2/3 of the island is US National Park (protected from over development), US property rights and contract law. St. John is a magical place that captured our hearts and we hope to retire here one day soon!
It was just the right size for us - and how refreshing it was to have no TV.
Yay! The view was spectacular, and that hammock could induce comas, I
swear. Ahhh.
We also loved Coral Bay, the food, snorkeling, people, pace, etc. What a great
spot. Those beaches were amazing! Anyway, we loved St. John. What a special
place. It was a great vacation--thanks!
Typical Caribbean wooden house. Great deck and hot tub. Messages sent to the owner were not forwarded to the managers, leading to minor communication snafus.
We come to St John, specifically Coral Bay, annually. Bambooshay was perfect for our needs! Tom , the property manager, is wonderful - kind, fun, and responsive! The villa was very clean and 100% met our needs. I would highly recommend. Private and well stocked with the essentials while still being close to our favorite South and North Shore beaches and all the conveniences of Coral Bay.
The island, the beaches, the villa everything was great!! We loved our stay and hope to come back soon!
My husband and I went there for our wedding/honeymoon.
We had an incredible time. They view is amazing. At night you can hear the music from a bar on the bay. It was magical. The main grocery store is next to where the ferries are. Word of advice is to stock up before heading here. It was absolutely beautiful.
We spent a week at Bambooshay for our 10th Anniversary and are so glad we did. The view from the spacious deck couldn't be beat and star gazing at night from the hot tub was amazing. We loved how private it felt though we were just up the hill from shops and restaurants. The inside was spacious, clean and nicely decorated though most of our time was spent outside. Having use of the beach towels, cooler, chairs and snorkel gear was fantastic. Others have mentioned mosquitos. We just kept the screen doors closed at all times and had no issues with them, even on the deck. When we arrived Tom was so friendly and helpful. It was nice knowing we could call him if we had any problems but we didn't need to. Coral Bay is great! So peaceful but still lots of restaurants and beaches to explore. Miss Lucy's and Concordia cafe were our favorites on that side of the island. We have so many memories from our visit and hope to stay at Bambooshay when we return. Do rent a jeep- the hills on the island make it a must!
What a great time my husband and I had at bambooshayvilla. It was the best idea to stay on the quiet side of the island. The house had everything you would need to making breakfast each morning to cocktails at night. The view alone is worth the stay not to mention the hot tub is an added bonus for star gazing at night. Best tip would be take a visit to Francis bay beach quiet beautiful and we saw turtles, many fish and a huge 6 foot sting ray on top of other rays. Just make sure at night you have the fans going or the net as masquitos can be a pain. We've been lots of tropical places including all of Hawaii but yet have decided this will be our one stop tropical stop for the future with family and friends just beautiful, quiet, and relaxing a little slice of national park heaven!!
St. John Recommendations
Best Beaches
All of the beaches along the north shore are awesome and if you have time, you should stop to see all of them that you can. Our favorites are the ones less travelled; farther away from town (Cruz Bay or "Love City" as it is known). Our favorite beach is probably Francis followed closely behind by Maho. Both are along the north shore and are closest in proximity to Bambooshay. Francis is relatively secluded and is usually one of the least crowded beaches along the north shore. The angle of the bay blocks most of the waves and the soft sand extends well out into the water with a gentle sloping angle. There are lots of little fish that will swim all around you and pelicans enjoy swooping in and dining on the smaller ones from time to time. Once you park your car and walk out facing the water just turn to the right and walk down to find a spot to set your chairs and towels. Also, if you stand still in the water you will find that the fish will come up to you and swim around your feet. Plus if you have some bread/chips/crackers you can feed them and they will be all around you in no time.
Maho is a lot like Francis but is right off the north shore road so it's easy to get in/out; especially if you're carrying heavy coolers, etc.
Trunk Bay has an underwater snorkeling trail that's pretty cool. This beach makes a lot of magazine covers and it often named as one of the top 10 beaches in their surveys.
Honeymoon Beach is a really nice beach close to Cruz Bay. To get to it, you can pay to park at Caneel Bay (the upscale Rosewood resort) or you can park at the Parks and Wildlife office near the dock and hike across. Take water if you hike! It's about a 30 minute hike.
Ms. Vie's beach is another good one. It's facing South and if wind/swells are affecting the beaches on the north shore, this is a good alternative. You have to pay Ms. Vie $2 for the privilege of using her beach, but it's worth it. While you are there make sure to try her garlic chicken and johnny cakes with honey.
Salt Pond also faces the south shore and is a very nice spot. Take your snorkel gear if you go here to see turtles, bright corals and a ray or two. It's a pretty long hike downhill so you'll want to lighten your cooler before hiking back to your car.
If you are really adventurous, check out Lameshur bay on the south shore. You HAVE to have a 4 wheel drive vehicle to get there. It's a very rough dirt road, but you may have the entire beach to yourself and it, like all the others, is beautiful once you get there.
Where to Eat
Our favorite spot in Coral Bay is Aqua Bistro. Be sure to ask if MJ is cooking! He is a very good chef from Boston and everything he makes is really good. If you're in the mood for a steak, he cuts his own.
You will inevitably end up at Skinny Legs. They have awesome hamburgers. They don't have a fryer, so you get chips with it. This is one of our favorite spots to hang out with our local friends.
Island Blues is bar food, but pretty good as well. They have live music a few nights every week and it has a great view right on Coral Bay.
Shipwreck Landing - Good conch fritters and drinks!
Miss Lucy's – awesome brunch on Sundays, very nice lunch menu as well. If you happen to be there during the monthly full moon party, you should check that out.
If you want a more upscale experience, try Sweet Plantains. The owner is famous for her curries, but everything is well prepared and very good. You need a reservation to go here.
If you make it to Cruz Bay, you have to check out Woody's Seafood Saloon. It's a favorite haunt of Kenny Chesney who owns a couple of houses on the island. One of our favorite restaurants on St. John is also in Cruz Bay – Lime Inn. Very good food and their whole jerked fresh snapper is excellent. They have a few parking spots reserved right out front. Parking spots are rare in Cruz Bay so that's a real benefit.
If you head over to Mongoose Junction they have a little restaurant called Sun Dog Café and while it may only be a few patio tables and a tiny shack where they cook, they produce some nice lunch time food and afternoon snacks.
Things to Do
Snorkeling – Definitely go to Leinster Bay and Waterlemon Cay. You park at the Anaberg Sugar Mill and hike about a mile along the beach to get there. It's our favorite snorkeling spot on the island with brilliant corals, turtles, squid, tons of reef fish and the sea floor is covered with golden starfish! You can rent gear from Crabby's in Coral Bay for a few bucks a day.
Get the full list of our recommendations upon booking your stay!
|Rate Period
|Weekend Night
My Standard Rate
4 night minimum stay
|Property Damage Protection
|Tax Rate
Please wait until you receive email confirmation from us that the dates are actually booked. In the unlikely event that we are not able to respond within 24 hours of submission, this system will show that you are booked, but we will personally respond as well to confirm that. Please do not book your flights until you get this personal confirmation. Cheers! Scott and Lori

subset: commoncrawl


text:
Gov. Jan Brewer's first look at Judge Ann Scott Timmer came in 1988. More than 20 years later, Timmer, a Court of Appeals judge, and Brewer, the governor, would meet three times in Timmer's quest to become a Supreme Court justice, with the third time being the charm.Read More »
Rep. Miranda, family, invoke Fifth Amendment during election-challenge suit
Rep. Catherine Miranda, her husband and step-daughter invoked their Fifth Amendment rights on the witness stand today in a court hearing to determine whether the Phoenix Democrat should be removed from office for submitting allegedly forged signatures on election forms. Miranda's attorney, Tom Ryan, said he can't allow his client or her family to testify while she is under criminal investigation.Read More »
Timmer's persistence pays off with state Supreme Court appointment
Judge Ann Scott Timmer's advice to her disappointed daughters after her third failed attempt at landing a spot on the Supreme Court was that the only way to avoid disappointment was to not take risks.Read More »
Crucial or crazy, Prop. 120 is the talk of northern Arizona
Rep. Chester Crandell said he was surprised when the first question of the Oct. 2 Clean Elections debate of Legislative District 6 candidates was on Proposition 120, the ballot measure he sponsored and is advocating.Read More »
Will scandals that forced 4 lawmakers from office influence voters?
Not since the AzScam scandal in 1991 have so many Arizona lawmakers left office amid criminal allegations in a single year.
The current scandals could cast a cloud over the state's general election. Lawmakers who leave in disgrace tend to discourage cynical voters from going to the polls, pollsters and players from the AzScam bribery scandal said.
Opponent sues to overturn Catherine Miranda primary victory
A Legislative District 27 Republican is using questions about forged signatures on Rep. Catherine Miranda's election forms to take the Phoenix Democrat to court to strip her of her primary election victory.Read More »
Dinner honoring Arredondo turned out to be a key to his downfall
Rep. Ben Arredondo, a Tempe Democrat, faces a sentence ranging from probation to 37 months in prison after pleading guilty this morning to two felony counts related to federal charges stemming from a public corruption indictment.Read More »
Former legislative staffer indicted on federal wire fraud charges; accused of stealing $131,000
A federal grand jury has returned a third indictment this year involving the Arizona Legislature as a former staffer was charged Sept. 25 with 15 counts of wire fraud for allegedly stealing from former House Speaker Jim Weiers' campaign account.Read More »
Change of plea set for Rep. Ben Arredondo
Rep. Ben Arredondo, a Tempe Democrat, is scheduled to plead guilty Friday to federal charges stemming from a public corruption indictment.Read More »
Maricopa County attorney argues Arizona abortion law is constitutional
A federal judge made the right call in concluding that Arizona's law banning abortions after 20 weeks of pregnancy is constitutional, Maricopa County Attorney Bill Montgomery argued in written briefs filed today with the 9th U.S. Circuit Court of Appeals.Read More »


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|Single Family: $249,990
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|Foreclosure: $242,216
4 Beds | 3.00 Baths | 0 sq. ft.
This property is an REO (Real Estate Owned). This is the final step in the foreclosure process. Ownership has reverted to the lender. This 0 square foot property has 0 bedroom(s) and 0 bath(s). The estimated sale price is $242,216.00. To access more extensive information on this property click the 'more property detail' link below. You will need to register for full access. Information listed with each property including ...
|Single Family: $242,000
3 Beds | 2.00 Baths | 2,936 sq. ft.
Stunning, open and upgraded home?hard to find 1-story with only large gameroom on second floor. Granite kitchen with maple cabinets and gas cooktop. Kitchen open to family room. Spacious formal dining. 3 bed, 2 ba, plus office w/ French doors, plus MIL/ guest / kids' room w/ second dedicated living room; plus third huge living/gameroom. Covered porch plus deck in nicely landscaped yard. 3 car tandem garage! Great neighbor...
|Single Family: $240,990
5 Beds | 3.00 Baths | 2,599 sq. ft.
Gorgeous two-story with Hickory Handscraped flooring, raised and staggered Maple Cabinets, Granite counter tops, Stainless Steel appliances, Covered patio, full Sprinkler System, & stone fireplace are just a few of the upgrades! Avalon offers many amenities including, a swim center, splash zone, basketball court, playscape and a covered picnic pavilion. Blackhawk Golf Course Nearby. On-site elementary school, Pfluger...
|Foreclosure: $240,617
5 Beds | 3.00 Baths | 4,557 sq. ft.
This property is an REO (Real Estate Owned). This is the final step in the foreclosure process. Ownership has reverted to the lender. This 4557 square foot property has 0 bedroom(s) and 0 bath(s). The estimated sale price is $240,617.00. To access more extensive information on this property click the 'more property detail' link below. You will need to register for full access. Information listed with each property includi...
|Single Family: $240,000
4 Beds | 2.00 Baths | 2,821 sq. ft.
HURRY,this one will not last! Looking for an backyard oasis for relaxing or entertaining? This is it! Spacious home offers a fantastic floor plan with all bedrooms plus utility room on the 2nd floor. Large master & spacious secondary bedrooms. First floor hosts a formal dining,formal living/study,family room with soaring ceilings,& dramatic 2-story windows,a huge kitchen with a cozy sun room as additional living s...
|Single Family: $240,000
4 Beds | 2.00 Baths | 2,770 sq. ft.
This is a Gorgeous Custom Built Highland Home! Professionally Landscaped on a Corner Lot, it backs up to a Greenbelt. The home is freshly painted with Semi-Gloss paint Inside and Out!. Beautiful Lighting Package, NEW! Laminate Floor in Office,Stairwell and Hallways Upstairs,Tile Entry to Dining and Kitchen AND New Carpet. Dual HAC, Front and Back Sprinkler System, All rooms wired with Cat5 High Speed Internet Cabling, L...
|Single Family: $239,900
4 Beds | 2.00 Baths | 2,778 sq. ft.
Remarkable corner lot property in golf course community. Recreational pool, lovely deck, 4/2.5, 3 living areas. Impeccable landscaping. Master Suite located downstairs~serene & private from rest of home. Open & bright concept in kitchen & family room. Gameroom/Media Room upstairs across from remaining bedroom/bath spaces. All bedrooms have walk-in closets & decorator colors add personality to each. Per...
|Single Family: $239,900
3 Beds | 2.00 Baths | 2,580 sq. ft.
Location, Location Location! Great 3-4 Bedroom home at end culdesac w/panoramic backyard! Open Entry w/Formal Dining, Warm Wood Flooring, Den open to Kitchen/Breakfast area! Granite Island Kitchen w/ plenty of cabinetry for the chef! Under mount Stainless Sink & Corner Pantry. Master Retreat w/open bath room, soaking tub, separate shower, and room for furniture! 4th bedroom or gameroom / study is spacious! Laundry roo...
|Single Family: $239,900
4 Beds | 3.00 Baths | 3,134 sq. ft.
Beautifully maintained home w/ 4 beds plus office, game room & great room. LARGE gourmet kitchen w/ island, stainless appliances & granite. Big backyard & no neighbors behind. Upgrades everywhere including flush mount speakers at game room, 3 bedrooms & baths, sprinkler system, fantastic landscape & extended back deck($16K), whole house carbon water filter, water softener, alarm system with cell connec...
|Single Family: $239,423
5 Beds | 3.00 Baths | 3,270 sq. ft.
Brand New Elevation, 8' front door, Juliet balcony, Front porch, Large Covered Patio with Cedar Railing, Study with glass french doors, art niches, 24X24 tile, Maple Cabinets, Granite Counter Tops, Custom Backsplash, Nice Bay Window in Master Bedroom, Over sized gameroom upstairs, 4 bedrooms, 2 Jack and Jill bathrooms upstairs! GREAT home, GREAT price!
|Single Family: $238,900
4 Beds | 3.00 Baths | 2,859 sq. ft.
Immaculate Home, SHOWS LIKE A MODEL. Barely lived in, built in 2010! Granite counters, stainless steel appliances, high ceilings. this home has it all. Save thousands vs. buying new!! Incredible energy package resulting in very low utility bills.14 SEER AC, Radiant Barrier. A must see!!
|Foreclosure: $238,578
4 Beds | 3.00 Baths | 0 sq. ft.
This property is an REO (Real Estate Owned). This is the final step in the foreclosure process. Ownership has reverted to the lender. This 0 square foot property has 0 bedroom(s) and 0 bath(s). The estimated sale price is $238,578.00. To access more extensive information on this property click the 'more property detail' link below. You will need to register for full access. Information listed with each property including ...
|Single Family: $237,879
5 Beds | 3.00 Baths | 3,237 sq. ft.
Beautiful two-story 5 bedroom home on a cul-de-sac lot. Features large master down and formal dining room. Oversized utility room and large pantry in kitchen. Upstairs are 4 bedrooms, Media Room and game room. 3 1/2 baths.
|Single Family: $236,000
6 Beds | 3.00 Baths | 3,931 sq. ft.
Well maintained, one owner, large family home with lots of space including gameroom upstairs. Master down; formal iving dining; large breakfast area overlooking the backyard. Hard tile and carpet throughout. Kitchen features all stainless appliances and gas range. You won't want to miss viewing this home.
|Single Family: $235,500
5 Beds | 3.00 Baths | 2,750 sq. ft.
Gorgeous two-story, Hickory Handscraped flooring, Raised & Staggered Birch Cabinets, Granite counter tops, Stainless Steel appliances, Covered patio, Full Sprinkler System, Stone Fireplace just a few of the upgrades! Avalon offers many amenities including, a swim center, splash zone, basketball court, playscape and a covered picnic pavilion. Blackhawk Golf Course Nearby. On-site elementary school, Pflugerville ISD. 1...
|Single Family: $235,000
4 Beds | 2.00 Baths | 3,156 sq. ft.
Bonus room down stairs can be used as a 5th bed room. Kitchen has corian countertops and 42" cabinets with extensive kitchen storage shelving. Master bedroom with sitting area, master closet is as large as bedroom. Master bath features a garden tub with separate shower, double-vanity. GREAT size game room upstairs, there is PLENTY of room for everyone in this home. Awesome OVERSIZED back patio. Great back yard comes with...
|Single Family: $234,900
4 Beds | 2.00 Baths | 2,824 sq. ft.
Must see this updated Meritage Home*So many great features*Master down w/roomy master bath*huge walk in closet*sep shower and tub*Game room up AND Theatre Room*AND office down at entry*Great use of the sqft*Open kitchen w/granite countertops*42in. cabinets*Breakfast bar for quick meals*Inside utility room*Garage w/extra storage*Formal dining great for entertaining*other bedrooms have walk in closets*Fenced backyard for ba...
|Single Family: $234,500
4 Beds | 3.00 Baths | 3,114 sq. ft.
Rare find! Classic elegant Streetman home in a super convenient location. Timeless features like soaring cathedral ceiling and spiral staircase adds unparallel details. Gourmet kitchen with built in appliances, granite countertops, upgraded cabinets and island. Barber carpe through out, Tens of thousands of upgrades. View of the greenbelt and field behind with trails. Great modern floor plan with master down and 3 bedroom...
|Single Family: $232,000
5 Beds | 3.00 Baths | 2,980 sq. ft.
**REDUCED**MOTIVATED SELLER**BRING ALL OFFERS**$1000 BONUS to BA if under contract by 5-30-12*Stunning floorplan!This home is loaded with upgrades-Master is down, granite in kitchen, 2.5 car garage, ornamental iron staircase! 1st floor wired for stero, gameroom floor is reinforced for pooltable,bay window. Travertine tile throughout! Amazing backsplash in the kitchen that matches the fireplace backsplash-Come take a look...


text:
Accidents Happen [Blu-ray] [English] [2009]
- Release Date: 07/27/2010
Best Buy is dedicated to always offering the best value to our customers. We will match the price, at the time of purchase, on a Price Match Guarantee product if you find the same item at a lower price at a Designated Major Online Retailer or at a local retail competitor's store.Here's how:
- If you find a qualifying lower price online, call 1-888-BEST BUY and direct a customer service agent to the web site with the lower price, or when visiting a Best Buy store, one of our employees will assist you.
- On qualifying products, Best Buy will then verify the current price to complete the price match.
Exclusions apply including, but not limited to, Competitors' service prices, special daily or hourly sales, and items for sale Thanksgiving Day through the Monday after Thanksgiving. See the list of Designated Major Online Retailers and full details.
Special Features
- "When Featurettes Happen"
- "Glorious Gloria" Featurette
- Cast & Crew Interviews
- Teaser
- Trailer
A dysfunctional New England family relies on the son prone to witnessing horrible accidents to get them though the hard times in this wry period comedy drama set in the 1980s. Billy Conway never set out to see the gruesome side of life, he just always happened to be around when it presented itself. One day, a terrible accident befalls the Conways, leaving one child dead and another severely brain-damaged. In the aftermath, Billy's dad leaves home. Caught between his troubled brother and his embittered mother, young Billy begins to stray from his good-son roots by hanging out with the neighborhood troublemaker. Who would have suspected that Billy's bad behavior could be the key to helping his family move on from the past and start life anew? ~ Jason Buchanan, Rovi
Cast & Crew
- Geena Davis - Gloria Conway
- Harrison Gilbertson - Billy Conway
- Joel Tobeck - Ray Conway
- Harry Cook - Larry Conway
- Sebastian Gregory - Doug Post


text:
Nadal coming to terms with rankings slide
By Tim Hanlon
BARCELONA (Reuters) - This is normally the time of year when Rafa Nadal comes into his own, honing his formidable claycourt skills in preparation for Roland Garros and leaving a trail of brutalized opponents to pick themselves up off the red clay.
Yet things have not gone to plan this season. While last year's injury woes seem to have passed, their legacy is a lack of form and a drop in confidence that have left him struggling for results.
Since hitting the big time in 2005, when he won the first of his record nine French Open titles, Nadal has never entered the fifth month of a season with only one solitary title -- that too a low key tournament in Buenos Aires where the highest ranked opponent he faced was world number 59 Federico Delbonis.
The Spaniard, who also owns eight Monte Carlo and eight Barcelona Open trophies, is now preparing to play at the Madrid Open in front of his home fans on the back of his worst run of results.
Desperately hoping to pick up form ahead of the French Open later this month, he is facing up to the fact that his ranking is on the slide.
He has not finished the season outside the top five in more than 10 years, but is now number four and heading in the wrong direction.
"We will see how far I have fallen after Roland Garros," Nadal told reporters in Madrid.
"This is part of sport. Other times I have been able to maintain a high ranking because I have returned quickly but this has not been the case now. Continued...


text:
News By Tag
Industry News
News By Place
Industry News
Are You Getting the Full Experience? Alpin Haus Releases Apps List For Customers
As more and more mobile users turn to apps to find the information they want and need, Alpin Haus have compiled a list of the best apps for our customers.
For Skiers/Snowboarders:
1. Alpine Replay (http://www.alpinereplay.com/
2. OnTheSnow Ski & Snow Report- (http://www.onthesnow.com/
3. Liftopia (http://www.liftopia.com/
For RVers:
1. RV Parks (https://itunes.apple.com/
2. Gas Buddy (http://gasbuddy.com/
3. AccuTerra (http://mobile.accuterra.com/)
For Boaters:
1. Catchbook- From Field & Stream, this app is a social journal which allows users to predict patterns in their favorite fishing spots by recording their catches based upon weather conditions, time of year, etc. Share photos of catches with friends. Compatibility:
2. BoatUS (http://www.boatus.com/
3. Boat Logger- Keep track of your trips right down to fuel cosumption. Compatibility:
For Pool Owners:
1. Swimming Pool Guide Pro- Helpful tips for everything from cleaning, to maintaining water, to closing up for the season. Compatability:
2. My Pool (http://www.heatsiphon.com/
3. Swim-Tracker- Log swim routines, track progress, and learn how to make improvements. An added bonus…daily motivational quotes. Compatibility:
For Snowmobilers:
1. Snowmobile.com- Snowmobile.com'
2. Ski Doo (https://itunes.apple.com/
3. Google Maps (http://www.google.com/
For Hot Tub Owners:
1. Spa Helper Pro (http://appshopper.com/
2. iTub (http://www.bavoo.com/
3. Pandora (http://www.pandora.com/
For Fitness:
1. Couch-to-5K (http://www.active.com/
2. RunKeeper- Features both GPS and manual activity tracking to log workouts. RunKeeper includes both iPod synchronization and audio clues during runs and social sharing functions for after. Compatibility:
3. fitID- Track, discover and share your fitness. Meals, workouts and progress are all logged. New diets and fitness trend content may be explored and social functions help share progress with friends and family. Compatibility:
Account Email Address Account Phone Number Disclaimer Report Abuse
Page Updated Last on: Feb 15, 2013


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- http://d2ob0iztsaxy5v.cloudfront.net/product/750109/7501095870_zm.jpg
€44.00 with free delivery*
Check, Reserve or Buy store stock
Slick skate styling doesn't stop at your feet as the Vans Old Skool II arrives to compliment your look. The navy fabric Backpack features a large main compartment, a handy front pocket, along with adjustable padded straps. Branding completes.
- Fabric
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- 42cm x 30cm x 12cm
Material: Fabric
Colour: navy
Product Code: 7501095870
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text:
Tahrir Square violence enters a third day – in pictures
Clashes between protesters and police have broken out in in Tahrir Square and looks set to intensify. Demonstrators were attacked by the army overnight – with some 20 people killed, according to reports. Field hospital doctors say they treated scores of people with injuries from excessive use of teargas and rubber bullets. Protest has spread all over Egypt during the build-up to elections, with many candidates announcing they would be suspending their electoral campaigns


text:
Decitabine and Vorinostat Conditioning Followed by CD3-/CD19- NK Cells Infusion for High Risk Myelodysplastic Syndromes
- Full Text View
- Tabular View
- No Study Results Posted
- Disclaimer
- How to Read a Study Record
Myelodysplastic Syndrome
Drug: Decitabine
Drug: Vorinostat
Biological: Interleukin-2
Other: Natural killer (NK) cells
Phase 2
|Study Type:
|Study Design:
|Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Treatment
|Official Title:
|Decitabine and Vorinostat With CD3/CD19 Depleted Haploidentical Donor Natural Killer (NK) Cells for the Treatment of High Risk Myelodysplastic Syndromes (MDS)
- Objective Response Rate [ Time Frame: After 2 Courses of Treatment (Approx. 3 months) ]Defined as Complete response (CR) + partial response (PR) + hematologic improvement (HI).
- Safety and Tolerability [ Time Frame: Day 1 through Month 3 ]Assess the safety and tolerability of Decitabine and Vorinostat followed by an infusion of CD3-/CD19-donor natural killer (NK) cells and a short course of IL-2 when given in the outpatient setting in patients with high risk myelodysplastic syndrome (MDS).
- Number of Patients Who Became Transfusion Independent [ Time Frame: 4-6 Months Post Start of Cycle 1 ]
- Natural Killer Cell Expansion [ Time Frame: After Cycle 2 (approx. 3 months) ]Defined as patients with in vivo expansion.
- Overall Survival [ Time Frame: 1 Year ]Patients alive at 1 year.
|Study Start Date:
|March 2013
|Estimated Study Completion Date:
|July 2016
|Primary Completion Date:
|October 2015 (Final data collection date for primary outcome measure)
|Assigned Interventions
Experimental: Patients With High Risk MDS
Patients who received treatment for high risk myelodysplastic syndromes (MDS). Treatment Received: Decitabine 10 mg/m^2/day intravenous (IV) over 1 hour days 1-5; Vorinostat 200 mg by mouth (PO) twice a day days 6-15; Il-2 activated donor natural killer cells (NK) infusion IV over 15 to 60 minutes day 17; Interleukin-2 6 million units subcutaneous (SQ) 3 times a week for 3 doses beginning day 17. Repeat treatment course 6 to 8 weeks after cycle 1 start date.
Drug: Decitabine
administered intravenous (IV), 10 mg/m^2/day over 1 hour on days 1-5.
Other Name: DacogenDrug: Vorinostat
200 mg by mouth (PO) twice a day on days 6-15
Other Name: ZolinzaBiological: Interleukin-2
6 million Units subcutaneous (SQ) 3 times a week for 3 doses beginning day 17
Other Name: IL-2Other: Natural killer (NK) cells
infusion intravenously (IV) over 15 to 60 minutes day 17
Detailed Description:
A single donor apheresis will be collected on day 15 of cycle 1, enriched for NK cells with the large scale CliniMacs device (Miltenyi) and activated by overnight incubation with IL-2. After washing, the final NK cell product will be divided in two, with half given fresh on day 17 of course #1 and half stored frozen until day 17 of course #2.
Clinical response will be formally assessed 4-6 weeks after the start of 2nd course based on International Working Group (IWG) criteria; however, bone marrow evaluations will be completed to assess for any sign of significant disease progression between cycle 1 and 2.
|Ages Eligible for Study:
|18 Years to 75 Years (Adult, Senior)
|Sexes Eligible for Study:
|Accepts Healthy Volunteers:
Inclusion Criteria:
Diagnosis of high risk myelodysplastic (MDS) that meets one of the following disease classifications and is requiring treatment:
- International Prognostic Scoring System (IPSS) Category: INT-2 or High Risk
- WHO Classification: RAEB-1 or RAEB-2
- High risk cytogenetic abnormality as defined by presence of Monosomy 7, complex karytope, or monosomal karyotype
- WHO Based Prognostic Scoring System (WPSS): High or Very High Risk
- Patients may be untreated or have had a maximum of 2 cycles of hypomethylating agents (azacitidine or decitabine) without evidence of treatment failure as defined by progression to more advanced MDS Who classification or AML. Patients must not have received treatment for their MDS within 4 weeks of beginning the trial. Treatments allowed prior to that time include azacitidine or decitabine and hematopoietic growth factors. No prior AML-like induction therapy allowed.
- Age ≥ 18 years of age
- Eastern Cooperative Oncology Group (ECOG) Performance Status 0-2
- Available related HLA-haploidentical NK cell donor by at least Class I serologic typing at the A&B locus
- Have acceptable organ function within 14 days of enrollment
- Ability to be off prednisone and other immunosuppressive drugs for at least 3 days prior to the natural killer (NK) cell infusion
- Women of child bearing potential must agree to use effective methods of contraception
- Voluntary written consent
Exclusion Criteria:
- Pregnant or lactating.
- Prior 7 + 3 (cytarabine given continuously for 7 days with an anthracycline drug, such as daunorubicin or idarubicin given for the 1st 3 days of treatment) or other AML-type induction chemotherapy
- New progressive pulmonary infiltrates on screening chest x-ray or chest computed tomography (CT) scan that has not been evaluated with bronchoscopy (when feasible)
- Uncontrolled bacterial or viral infections - chronic asymptomatic viral hepatitis is allowed
- Pleural effusion moderate to large in size that are detectable on chest xray
- Known hypersensitivity to one or more of the study agents
- Prior hypomethylating treatment greater than 2 cycles or with documented treatment failure
- Prior use of histone deacetylase inhibitors
- Serious medical or psychiatric illness likely to interfere with participation in this clinical study in the opinion of the enrolling investigator
- Inability to swallow capsules
- Active human immunodeficiency virus (HIV)
- Other active and potentially life threatening malignancy excluding localized basal or squamous cell skin cancer, cervical carcinoma in situ, superficial bladder cancer, localized prostate cancer
Please refer to this study by its ClinicalTrials.gov identifier: NCT01593670
|United States, Minnesota
|Masonic Cancer Center, University of Minnesota
|Minneapolis, Minnesota, United States, 55455
|Mayo Clinic
|Rochester, Minnesota, United States, 55901
|Principal Investigator:
|Erica Warlick, M.D.
|Masonic Cancer Center, University of Minnesota
|Responsible Party:
|Masonic Cancer Center, University of Minnesota
|ClinicalTrials.gov Identifier:
|NCT01593670 History of Changes
|Other Study ID Numbers:
MT2012-04 ( Other Identifier: Blood and Marrow Transplantation Program )
|Study First Received:
|May 4, 2012
|Last Updated:
|January 22, 2016
Keywords provided by Masonic Cancer Center, University of Minnesota:
high risk myelodysplastic syndrome
natural killer cells
Additional relevant MeSH terms:
Myelodysplastic Syndromes
Pathologic Processes
Bone Marrow Diseases
Hematologic Diseases
Precancerous Conditions
Antineoplastic Agents
Histone Deacetylase Inhibitors
Enzyme Inhibitors
Molecular Mechanisms of Pharmacological Action
Antimetabolites, Antineoplastic
Analgesics, Non-Narcotic
Sensory System Agents
Peripheral Nervous System Agents
Physiological Effects of Drugs
ClinicalTrials.gov processed this record on May 23, 2017


text:
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Pencil In Some 'Me-Time' and Get a Swedish or Deep-Tissue Massage in Old Orchard Beach
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text:
You are currently using an unsupported web browser.
We support the latest versions of these browsers:
If you have any problems while attempting to download a browser, you should contact your organization's system administrator.
For any questions related to Cvent, feel free to contact our Customer Care team.
You immediately feel at home at the Art Nouveau style Hotel Montana. The hotel is located directly by the Davos Dorf railway station, and just one minute on foot from the Parsenn mountain railway. The Jakobshorn cableway and all trendy bars, clubs and shops can be conveniently reached using the loc...
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text:
That time Jaden Smith hid a Nike ad in his music video
Also, is Jaden a robot?
Get ready to break the bank.
This is your chance to go 'Back to the Future' for less than the cost of a movie ticket.
Bootleg versions of Nike's self-lacing 'Back to the Future' sneakers are already rolling off production lines from China.
Nikes with Power Laces are real and coming in 2016.


text:
It has a long and respected track record of working internationally on early childhood development and children's rights.
Host Institution(s):
Radio Netherlands Training Centre
Field of study:
Journalism related to early chilhood development and children's rights
Target group:
Journalists from qualifying countries (Mexico, Kenya, India (Orissa), Peru, Colombia, South Africa, Tanzania, Uganda and Caribbean Region) who have an interest in covering children's issues.
Scholarship value/inclusions:
Four-week, expenses-paid professional training course in the Netherlands.
Application instructions:
Send the following requirements to firstname.lastname@example.com by the deadline:
Contact information:
For any questions that are not addressed in the frequently asked questions page (see link below), contact the foundation at email@example.com for further guidance.
Application Deadline; Annual


text:
Congratulations! Now that you've got your free trigger point charts, if you want to go deeper into treating your triggers for pain relief - here's where to go next:
- A great FREE Trigger Point Chart Resource – see all the major muscles with their trigger points mapped out...
- Get self treatment techniques for trigger pain – even in those 'impossible to reach areas'
- What to do about SEVERE and chronic Trigger Point muscle spasms…
- Get safe stretches and exercises to help you build muscle strength and begin your normal active life again…
Instructions: Just click on the part of your body that is painful, and then click on the muscle names to see which trigger points could be causing your pain.
Use the charts to find trigger points anywhere in your body. Trigger points re small areas of spasm within your muscle. They can cause pain at the site of the trigger point - or in another area.
Fortunately, these pain referall patterns remain constant in different people, so they have been carefully mapped. This means that you can use the images above to find which trigger points may be responsible muscle pain you may be experiencing.
To begin, select the front or back of the man in the picture - depending on where you feel your pain. Then click on the area where you are feeling pain.
When you do this, a selection of different muscles will appear - all possible candidates for the cause of your pain.
The next step involves going through each muscle, and seeing if the pain patterns in the diagrams correspond to the pain you feel.
So - click on one of the muscle names and you will to through to a more detailed picture of that muscle. There will be X's - these show the location of trigger points in that muscle.
When you run your cursor over the X's the pain referral pattern of the trigger point will be shown in red. You can then use your own judgement to see whether that distribution of pain is the same as what you are feeling.
If so, then it's likely that you have these trigger points.
If you don't know how to examine for trigger points, then watch this video to get detailed instructions.
Trigger points are a little known, but at times signification source of pain for many people. Treating them can reduce chronic pain, and increase flexibility and ease of movement.
Knowledge of how to find and release trigger points is a valuable skill you can use to improve your quality of life - as well as the fitness and health of those around you.


text:
€ 32,99
Bisher € 34,93
Lieferbar innerhalb von 2-3 Tagen
Januar 2006
BeschreibungPHPEclipse brings PHP functionality to the Eclipse platform, enhancing it as an environment for developing web applications and focusing on what PHP web developers need to create an application. PHPEclipse's development is active and its tool-set provides everything you need to write web applications in PHP.
The fusion of Eclipse, the leading open source development environment, and PHP is an exciting prospect for web developers. This book makes sure that you are up and running as quickly as possible, ready to take full advantage of PHPEclipse's tuned PHP development tools, without requiring any prior knowledge of Eclipse.
You will begin with installing and configuring PHPEclipse, before moving onto a tour of the Eclipse environment, familiarizing you with its main components. As a plug-in to Eclipse, PHPEclipse is able to harness the platform to provide a rich and powerful development experience.
For helping you improve the efficiency of your PHP coding, the book details the powerful editing features of PHPEclipse, and shows you how to use it to better organize your application code.
You will see how PHPEclipse helps you throughout the development lifecycle, and learn how to use PHPEclipse's debugger to troubleshoot and step through your PHP code as it executes. The book rounds off with coverage of accessing databases and managing source code from within the Eclipse environment. For the final step for your application, you will learn how to deploy your to a production server.
Features include : debugging, using PHPDocumentor, the QuantumDB plug-in, managing your source code repository, deployment using FTP, SFTP, WebDav and Ant.
Shu-Wai Chow has worked in computer programming and information
technology for the past eight years. He started his career in
Sacramento, California, spending four years as the webmaster for
Educaid, a First Union Company and another four years at Vision Service
Plan as an application developer. Through the years, he has become
proficient in Java, JSP, PHP, ColdFusion, ASP, LDAP, XSLT, and XSL-FO.
Shu has also been the volunteer webmaster and a feline adoption
counselor for the Sacramento Society for the Prevention of Cruelty to
Animals. He is currently a software engineer at Antenna Software in
Jersey City, New Jersey.
Born in the British Crown Colony of Hong Kong, Shu did most of his
alleged growing up in Palo Alto, California. He studied anthropology
and economics at California State University, Sacramento. He lives
along the New Jersey Coast with seven very demanding cats, three birds
that are too smart for their own good, a cherished Fender Stratocaster,
and a beloved, saint-like girlfriend.
Untertitel: A User Guide. Paperback. Sprache: Englisch.
Verlag: Packt Publishing
Erscheinungsdatum: Januar 2006
Seitenanzahl: 228 Seiten
Format: kartoniert


text:
Chris Hemsworth Risks His Life to Race in First 'Rush' Trailer
Universal Pictures has unveiled the first trailer for Ron Howard's "Rush". Based on the real-life rivalry between Formula 1 drivers James Hunt and Niki Lauda, the film will witness the fiercest competition that has ever been written in racing history as seen in the video.
Among all racers, Chris Hemsworth's brass and daring Hunt only keeps his eye on Niki Lauda, the established Austrian champion who is portrayed by Daniel Bruhl. The clip later sees how far the two racers take their intense rivalry to as glimpses of crash and Lauda getting injured are previewed.
"Rush" is a racing drama which is set against the sexy, glamorous golden age of Formula 1 racing in the 1970s. The story follows handsome English playboy James Hunt and his methodical, brilliant opponent Niki Lauda who were willing to risk everything to become world champion in a sport with no margin for error.
Also starring Olivia Wilde as Hunt's wife named Suzy Miller, the film is penned by Peter Morgan and accompanied by Hans Zimmer's score. The film, which also includes Natalie Dormer, Alexandra Maria Lara and Christian McKay in the cast ensemble, will race to U.S. theaters on September 20.
- 'Logan' Producer Hints at X-23 Solo Movie
- Jamie Foxx Called 'Disrespectful' by Nyle DiMarco for Making Up Sign Language on 'Fallon'
- John Boyega Play in London Cut Short Due to Bomb Threat
- Joseph Duggar Engaged to Kendra Caldwell at His Sister Joy-Anna's Wedding
- Gregg Allman Dies After Battle With Liver Cancer, Ex-Wife Cher Mourns Him
- Watch a Preview of Halsey and Lauren Jauregui's 'Strangers' Video
- Beyonce Channels Michael Jackson in Throwback Photos
- Blac Chyna Licks Christina Milian's Boob in NSFW Pic


text:
PARIS, Sept 6 (Reuters) - French automotive parts maker Valeo said on Tuesday it plans to repurchase up to 670,000 additional shares at a total of up to 22.5 million euros The plan follows the announcement on Aug. 16 of a buyback programme of up to 610,000 shares at a total value of 18.5 million euros. The price of the latest repurchase will not exceed 70 euros per share, Valeo said. The shares will be used towards stock option plans, the allotment of shares to employees and eventual ...
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Liberal Opinion Week
What happens when the factories and the steel mills don't come back? When the coal mines fail to reopen? When both a tightfisted Congress and the government of Mexico refuse to pay for his boondoggle of a border wall?
When the president-elect, Donald Trump, takes office and has to confront inconvenient reality, how will he react? "We owe him an open mind and a chance to lead," Hillary Clinton said Wednesday, and of course she is right. But I wouldn't be honest if I pretended, at this point, to be hopeful. My fear is that the man we saw on the campaign trail is the same man we will see in the White House.
He proved to be a tremendously effective demagogue. He stunned the world by energizing and mobilizing legions of "forgotten men and women" -- white, working-class Americans living in small towns and rural areas across the nation -- who bought into his pledge to "make America great again." Instead of serious policy proposals, he gave them scapegoats: immigrants, Muslims, people of color living in "inner cities" that he imagined as circles of Dante's hell.
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Some 27 years ago today, the world was stunned: On the night of Nov. 9, the Berlin Wall, the living symbol of Cold War divisions, was suddenly breached. After an East German official vaguely announced that travel restrictions would be eased, border guards, confused by their orders, did nothing to stop excited East Germans from crossing over. Soon images of Germans ecstatically swarming the wall, chipping away at it with hammers, and hugging long-separated friends and strangers alike, flooded televisions around the world.
Few saw it coming. Yet the sudden implosion of the Communist bloc in eastern Europe and the fall of the wall marked what was widely heralded as a historic milestone in the spread of Western, democratic values.
Now, another surprise moment is playing out, thanks to the stunning victory early Wednesday of Donald Trump, a candidate who began his presidential campaign promising to build a wall on the border with Mexico.
President-elect Trump?
Well, whaddaya know. There really was a "hidden Trump vote" after all.
For months almost every opinion poll had Democrat Hillary Clinton firmly holding onto her No. 1 position in the presidential race. No problem, said Republican Donald Trump's team, which claimed a "hidden vote": Trump voters who didn't want to admit their choice to pollsters.
Preposterous thought. Numerous pollsters with sophisticated computers constantly belching out data, there was no way a hidden block of voters was eluding their scrutiny.
Until President-elect Trump.
Somewhere, I imagine, Mike Royko is smiling.
The late Pulitzer Prize-winning columnist and champion of working stiffs in his beloved Chicago and elsewhere used to be so annoyed by political pollsters that in the early 1980s he urged his people to lie to them.
Hillary Clinton's use of the word "deplorables" to refer to half of Trump's supporters has taken a familiar tone when it comes to election contests. Partisans often view the other side's voters as immoral, ignorant and irrational.
While research tells us that humans are subject to any number of biases, shortcomings and prejudices, support for Trump - or Clinton - need not, and probably should not, be attributed to these sources. Instead, the patterns of support for the two major candidates reveal a public that tends, on average, to match their votes to a range of rational, competing interests.
Some commentators, for example, wondered why white evangelical Christians supported Donald Trump despite his marital record and his remarks and alleged sexual assaults against women. Yet Trump nonetheless was clearly more likely than Clinton to advance the objectives typical of white evangelicals, including the appointment of pro-life federal judges.
He was Donald Trump before Trump — his political godfather. The racial profiling, the authoritarian streak, the robust defense of easily refutable lies — all are part of the repertoire of Sheriff Joe Arpaio of Maricopa County, Arizona.
On Tuesday, the man who was emblematic — at least in the Southwest — of Trump's attempt to hold back the demographic tide of the new America was resoundingly defeated. It was a vote for decency, for common sense, and no small amount of revenge from many of the victims of his strong-arm policies.
After six terms as the chief lawman of the most populous county in Arizona, Arpaio was defeated by a former Phoenix police officer, Paul Penzone, a Democrat.
"There's a new sheriff in town," Penzone said. You could say that time, and federal law, finally caught up with the 84-year-old sheriff. He's been under court order to stop targeting Latinos. Last month, federal prosecutors charged him with criminal contempt for allegedly defying that court order.
In 1777, when Britain received words of the drubbing its forces had suffered at Saratoga to the American rebels, a friend of Adam Smith's exclaimed that "the nation was ruined." The wise philosopher calmly replied: "There is a great deal of ruin in a nation." That proposition is about to be put to the test by President-elect Donald Trump.
Yes, I can barely believe that I am actually writing those words: "President Trump." I never thought he was remotely qualified for the highest office, and I never thought he would win. I was obviously wrong about the latter. Now I have to pray that I was wrong about the former.
Nov. 9, 2016, is a dark, depressing day for me and for the slim popular majority of Americans who voted for Hillary Clinton. It is easy on a day like this to fall prey to one's worst fears. Is this the dark night of fascism descending on America? Maybe. Is this the triumph of white supremacists? Could be. Is this the end of NATO and the triumph of Vladimir Putin? Quite possibly. I admit that I am deeply worried that these cataclysmic scenarios could actually come to pass. This really could be Apocalypse Now.
Women, as Hillary Clinton showed Wednesday morning, know how to absorb pain. And Clinton has had plenty of practice about how to stand before the cameras after public humiliation.
Clearly, she needed time to compose herself. She made no appearances overnight, instead calling Donald Trump to concede. But when she strode onstage in purple and gray, Bill Clinton behind her in a purple tie, her voice did not waver.
Women seldom have the luxury of giving in to pain. Many have children or grandchildren or aging parents to tend, whether they themselves are sick or in emotional turmoil.
So Clinton faced her despondent campaign staff and her despondent half of the country and tried to rally them. She spoke most directly to young people, and to women and girls. She had hoped to stand before them as a symbol of all that women could achieve. Now she had to demonstrate once again what women can endure.
"I've had successes and setbacks, sometimes really painful ones," she told them.
I began election night writing a column that started with words from an immigrant, my friend Lesley Goldwasser, who came to America from Zimbabwe in the 1980s. Surveying our political scene a few years ago, Lesley remarked to me: "You Americans kick around your country like it's a football. But it's not a football. It's a Fabergé egg. You can break it."
With Donald Trump now elected president, I have more fear than I've ever had in my 63 years that we could do just that — break our country, that we could become so irreparably divided that our national government will not function.
From the moment Trump emerged as a candidate, I've taken seriously the possibility that he could win; this column never predicted otherwise, although it certainly wished for it. That doesn't mean the reality of it is not shocking to me.
Sure, if you're in the approximately 52 percent majority of voters who supported someone other than Donald Trump, go ahead and mourn. When a former Ku Klux Klan leader like David Duke is giddily celebrating a political triumph for his values, how can we not ache for our own?
Yet, like it or not, we Americans have a new president-elect, and it's time to buck up. I've seen past elections that were regarded as the end of the world — including, in many Democratic circles, the Reagan triumph of 1980 — and the republic survived. This time as well, our institutions are stronger than any one man. We are not Weimar Germany.
It was disgraceful that many Republicans eight years ago tried to make President Barack Obama fail. That's not the path to emulate. Today, having lost, we owe it to our nation to grit our teeth and give President-elect Trump a chance.
So he won. The nation takes a deep breath. Raw ego and proud illiteracy have won out and a severely learning-disabled man with a real character problem will be president. We are so exhausted from thinking about this election, millions of people will take up leaf-raking and garage cleaning with intense pleasure. We liberal elitists are wrecks. The Trumpers had a whale of a good time, waving their signs, jeering at the media, beating up protesters, chanting "Lock her up" -- we elitists just stood and clapped. Nobody chanted "Stronger Together." It just doesn't chant.
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Liberal Opinion Week
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text:
Disclaimer: I don't own Loveless
"You'll come crawling back"
"When you see that Seimei is not the right sacrifice, the right person for you… You'll come crawling back." Ritsuka spat through the blinding gleam of tears, cursing his brother's name for the first time.
The bitter words escaped his lips before a clear thought could reach his mind. At any rate, there was no way he could think clearly after what his fighter- ex fighter told him a moment before.
Seimei returned and took Soubi back. "You've played enough", he said then commanded his fighter to leave Ritsuka and follow him again. Soubi obeyed.
Soubi and Ritsuka stood several paces apart on an empty street. Soubi stepped forward, reaching out. "Ritsuka" A cool breeze blew several strands of flaxen hair into his face. The sky grew dim and bleak; it seemed a storm was approaching.
Ritsuka turned his gaze upward, deep violet eyes locking with the fighter's. "Do you honestly think there's anything you can say right now that will make me feel better, that will make anything alright?!" He cried in anguish.
A rotten taste came to his tongue and he wanted to spit, even at the offending man before him, but swallowed. All this talk was rotten in his mouth. It was unbearable, everything was so frustrating, confusing, upsetting, and he couldn't take it. Suddenly he let loose a long scream.
Soubi didn't budge at the loud outburst. Afterward he spoke calmly, in a coaxing manner, "Ritsuka… Seimei is-"
Ritsuka cut him off with a glare and an angry "Don't".
He continued, "Seimei is my master".
"I cannot go against his will Ritsuka".
"Stop it!" he screamed. "Stop talking, stop saying my name!" It sounded like such poison now. Every word Soubi ever uttered was a lie. Each breath of "I love you" was trickery. The fighter made spells from words; it was no surprise the spell of love was just the same.
Heated, Ritsuka spoke through gritted teeth, "You manipulated me, lied to me over and over again, and now I'm stuck. Bastard you did this to me, and for what?" Dark hair became tangled as he shook his head.
"You made me… you made me love you! How could I be such an idiot?" He smacked hands over his damp face.
The sound of a chunk of tar rolling brought his hands swiftly down. Knowing without opening his eyes, he pushed Soubi who was now a few inches in front of him.
"Don't touch me" he whispered bitterly with a glare. "Go. You'll see, he'll treat you like dog shit and never show you love! You'll realize and come crawling back to me. But I'll tell you something, if you walk away now, if you go to him, I won't take you back."
The fighter remained silent.
His reticence infuriated Ritsuka. Frustration, anger, and most of all sadness filled him, causing waves of tears to shine on pale skin.
"Go away I don't ever want to see you again!" He screamed then turned away, hands clenched at his sides. Moments later, he heard the clicking sound of shoes connecting with pavement. Emptiness surrounded him now.
Minutes later from a distance he heard the soft sound of Soubi's voice, "Goodbye Ritsuka".
He clamped a hand over his mouth to choke back a sob before it escaped and broke his stance.
In the silence and darkness Ritsuka stood alone.
You'll come crawling back… and I hope I can have you…
Hope you liked it, please review.


text:
Signaling and cellular microbiology
Diguanylate cyclases (DGCs) regulate biofilm formation and motility in bacteria by synthesizing the second messenger cyclic di-GMP (c-di-GMP) in response to environmental stimuli. DGC enzymatic activity is believed to be dependent on the presence of a GG(D/E)EF active site motif, however approximately 25% of known DGCs contain a degenerate active site. The
Ectopic expression of VCA0965 in
Based on these results, we conclude that VCA0965 is capable of c-di-GMP synthesis and that the first amino acid of the GG(D/E)EF motif is more tolerant of substitutions than currently appreciated.
Cyclic di-GMP (c-di-GMP) is a bacterial second messenger that is widely utilized by bacteria, with more than 80% of sequenced bacteria predicted to use this signal
DGCs are characterized by the presence of a conserved GGDEF domain composed of approximately 200 amino acids
One explanation for the high frequency of degenerate DGCs is that these proteins in some cases act as c-di-GMP receptors by binding to c-di-GMP at either their degenerate active site or their RXXD inhibition site. These degenerate DGCs respond to c-di-GMP in several ways. C-di-GMP may mediate participation of the DGC in a regulatory cascade, as does PopA from
Here we investigate the degenerate
Bacterial strains and culture conditions
Strains used in this study are listed in Table
Motility assay
Motility plates (60 mL) were prepared immediately before each assay from LB supplemented with 0.35% agar. Plates, supplemented with 100 μg mL-1 kanamycin and 0.1 mM isopropyl-β-D-thiogalactoside (IPTG) as appropriate, were allowed to solidify for 1 to 4 hours prior to use. Plates were inoculated from overnight cultures by stabbing through the agar with a pipette tip, and left undisturbed at room temperature for 1 hour before inversion and incubation at 37°C for 20 hours. Motile bacteria were digitally imaged using an Alpha Innotech Red Gel Imager with white light and an 800 ms exposure time. Images were processed to calculate colony area by determining differential pixel density using ImageJ software
Figure 1
Motility repression by
Motility repression by
Minimum biofilm eradication concentration (MBEC) and flow cytometry biofilm assays
MBEC plates were prepared by inoculating 150 μL of LB/well with a 1 to 1000 dilution from overnight
Determination of the intracellular concentration of c-di-GMP
Cultures were prepared by diluting an overnight culture of
Protein purification
An overnight culture of
Determination of DGC activity
Purified proteins were diluted 1/25 and mixed with 0.015 mM GTP in reaction buffer (41 mM NaCl, 24 mM Tris-Cl, 1.25 mM MgCl2) for 24 hours at room temperature. Samples were then boiled for five minutes until precipitate formed and centrifuged at 15,871 g for 10 min. to remove precipitated proteins. The concentration of c-di-GMP was determined using LC-MS/MS as described above.
Census of DGCs that regulate motility in
The active site of VCA0965 is required for motility inhibition
We hypothesized that VCA0965 was incapable of synthesizing c-di-GMP and functioned as a c-di-GMP receptor protein to repress motility. Consistent with previously studied DGC effectors, c-di-GMP could bind VCA0965 at its degenerate active site (referred to as the A-site) and/or its RXXD allosteric inhibition site (referred to as the I-site)
Figure 2
VCA0965 requires an intact active site to repress motility
VCA0965 requires an intact active site to repress motility. Quantification of motility as determined by quantifying colony area (cm2) in
VCA0965-I induces biofilm formation
Like many bacterial species, biofilm formation in
Figure 3
Overexpression of the VCA0965-I mutant induces biofilm formation in an A-site dependent manner
Overexpression of the VCA0965-I mutant induces biofilm formation in an A-site dependent manner. (A) Mean biofilm formation of
To confirm these results, we measured biofilm formation using flow cytometry as previously described
VCA0965 synthesizes c-di-GMP
The above findings that VCA0965 and/or its I-site mutant were able to repress motility and induce biofilm formation only with an intact AGDEF active site suggested that VCA0965 was capable of influencing intracellular c-di-GMP levels. To test this hypothesis, we measured the impact of VCA0965, VCA0965-I, VCA0965-A and VCA0965-AI overexpression on intracellular c-di-GMP levels in
Figure 4
VCA0965 increases the intracellular concentration of c-di-GMP
VCA0965 increases the intracellular concentration of c-di-GMP. Wild type VCA0965 and its mutant derivatives were overexpressed in
It remained possible that VCA0965 modulated c-di-GMP levels by interacting with other components in the cell, although these components would have to be conserved between
Figure 5
VCA0965 (180–396) is an active DGC
VCA0965 (180–396) is an active DGC
Determining the plasticity of the first amino acid in the active site of VCA0965
As a naturally occurring enzymatically active AGDEF DGC has never been reported, we wondered what amino acid substitutions in the first residue of this active site would maintain DGC activity (amino acid residue 284 in VCA0965). To answer this question, we constructed mutants of VCA0965 containing all 19 amino acids in the first position of the AGDEF active site and determined if expression of these proteins in
Figure 6
The active site of VCA0965 tolerates additional amino acid substitutions
The active site of VCA0965 tolerates additional amino acid substitutions. (A) Substitution mutants of every amino acid in the first position of the AGDEF active site of VCA0965 (residue 284) were constructed and examined for the ability to reduce motility of
The activity of the glycine, histidine, and methionine substitutions was further examined by determining if induction of these proteins significantly increased intracellular c-di-GMP in
Bioinformatic analysis of AGDEF active sites
Our results studying VCA0965 suggest that the AGDEF active site is in fact capable of DGC activity. We determined the prevalence of this active site among GGDEF proteins using the standalone version of NCBI's PsiBlast to scan the 28,254 sequences of GGDEFs in the Pfam database
The N-terminal domains of GGDEF proteins typically encode conserved domains that are thought to bind environmental signaling cues to control the associated enzyme domain
Here we show that VCA0965, which encodes a degenerate AGDEF active site, is an active DGC that synthesizes c-di-GMP. Overexpression of VCA0965 represses motility by increasing c-di-GMP levels in
Retention of activity in ECA3270 and VCA0965 is surprising when the steric constraints of the active site are considered. Within the active site, the GGDEF motif is located in a hairpin
Contrary to what might be expected based on the structure of the DGC domain, degeneracy at the first amino acid is quite common among DGCs. 23.7% of all DGCs are degenerate at one or more amino acids in the GGDEF motif
Finally, we found that although VCA0965 is able to repress motility and increase intracellular c-di-GMP levels when overexpressed, WT VCA0965 did not impact biofilm formation. However, the lack of biofilm induction is inconsistent as we have found that overexpression of VCA0965 led to an approximately 6-fold increase in biofilm formation in another study
Our results highlight that the first amino acid of the GG(D/E)EF motif should be considered flexible in regards to DGC activity. In combination with the finding for ECA3270, a more accurate description of a functional active site should be (G/S/A)G(D/E)EF. However, whether enzymes which naturally encode a GG(D/E)EF will retain DGC activity upon substitution of the first glycine to serine or alanine remains to be determined. It is possible that intragenic epistatic interactions are necessary for these alterations of the first amino acid to remain functional. Additional non-canonical active site motifs should be assayed to determine if enzymatic activity of these DGCs is widespread. Furthermore, structural analysis of ECA3270 and VCA0965 can highlight how variance at the first amino acid of the GGDEF motif can accommodate DGC activity.
The research presented here shows that the AGDEF containing
DGC: Diguanylate cyclase; PDE: Phosphodiesterase; c-di-GMP: Cyclic di-GMP.
Competing interests
The authors declare no competing interests.
Authors' contributions
JLH, GBS and BJK collected and analyzed the data and JLH and CMW wrote the manuscript. All authors read and approved the final manuscript.
This work was supported by the MSU Foundation and NIH grants K22AI080937, U19AI090872, and U54AI057153 to C.M.W. J.L.H. would like acknowledge support from the Professorial Assistantship program at MSU and the American Society for Microbiology Undergraduate Research Fellowship program. We also would like to thank the Rudolph Hugh Fellowship and the Russell B. DuVall Scholarship to B.J.K and the Russell B. DuVall Scholarship to J.L.H. from the Microbiology and Molecular Genetics department at MSU. The authors would like to thank the Michigan State Mass Spectrometry facility for assistance in quantifying c-di-GMP.
Comparative genomics of cyclic-di-GMP signalling in bacteria: post-translational regulation and catalytic activity
Cyclic di-GMP: the first 25 years of a universal bacterial second messenger
GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility
Hfq-dependent, co-ordinate control of cyclic diguanylate synthesis and catabolism in the plague pathogen
The PilZ domain is a receptor for the second messenger c-di-GMP: the PilZ domain protein YcgR controls motility in enterobacteria
Cell-cell signaling in
Analysis of FimX, a phosphodiesterase that governs twitching motility in
LapD is a bis-(3′,5′)-cyclic dimeric GMP-binding protein that regulates surface attachment by Pseudomonas fluorescens p f0–1
Cyclic diguanylate (c-di-GMP) regulates
Role of cyclic Di-GMP during el tor biotype
Identification of FleQ from
Integration of Cyclic di-GMP and Quorum Sensing in the Control of
Riboswitches in eubacteria sense the second messenger cyclic di-GMP
An allosteric self-splicing ribozyme triggered by a bacterial second messenger
Cyclic diguanylate is a ubiquitous signaling molecule in bacteria: insights into biochemistry of the GGDEF protein domain
The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains
Novel domains of the prokaryotic two-component signal transduction systems
Allosteric control of cyclic di-GMP signaling
Second messenger-mediated spatiotemporal control of protein degradation regulates bacterial cell cycle progression
Structure of the cytoplasmic region of PelD, a degenerate diguanylate cyclase receptor that regulates exopolysaccharide production in
Discrete Cyclic di-GMP-dependent control of bacterial predation versus axenic growth in
FimX, a multidomain protein connecting environmental signals to twitching motility in
The orphan histidine protein kinase SgmT is a c-di-GMP receptor and regulates composition of the extracellular matrix together with the orphan DNA binding response regulator DigR in
YybT is a signaling protein that contains a cyclic dinucleotide phosphodiesterase domain and a GGDEF domain with ATPase activity
A multi-repeat adhesin of the phytopathogen,
The EAL domain protein YciR acts as a trigger enzyme in a c-di-GMP signalling cascade in E. coli biofilm control
Cell-cell signal-dependent dynamic interactions between HD-GYP and GGDEF domain proteins mediate virulence in
Toxin-coregulated pilus, but not mannose-sensitive hemagglutinin, is required for colonization by Vibrio cholerae O1 El Tor biotype and O139 strains
New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fischeri Reveal Patterns of Infection and lux Expression In Situ
NIH Image to ImageJ: 25 years of image analysis
Quantification of high specificity signaling
Transcriptome and phenotypic responses of
Quorum sensing controls biofilm formation in
Identificatin of small molecules that antagonize diguanylate cyclase enzymes to inhibit biofilm formation
Phosphorylation-independent regulation of the diguanylate cyclase WspR
The Pfam protein families database
Cyclic di-GMP signaling in bacteria: recent advances and new puzzles
Structural basis of activity and allosteric control of diguanylate cyclase


text:
Family Welcome Home Dinner
Company Store
Thursday, November 10, 2016
This event is sold out.
Family Welcome Home Dinner with Chef Pat Bagg
- Warm Winter Squash & Chick Pea Salad with Tahini Dressing
- Salt Baked Salmon with Red Onion Caper Vinaigrette
- Roasted Baby Potatoes
- Easy Chocolate Mousse with Cocoa Nib Brittle
To purchase seats: Please call the store directly to make reservations. (Seats cannot be reserved without payment.) Call early for reservations as class size is limited. Our classes begin at 6pm and each class lasts for about 2 hours.
Refund Policy: Stonewall Kitchen Cooking Class Fees are refundable and/or transferable up to 48 hours prior to the scheduled class. No refunds and/or transfers will be issued for class cancellations with less than 48 hours' notice. This cancellation policy is strictly enforced.
Private Parties: Whether it is for a bridal party, a birthday, a family party, or a corporate event, a cooking class is a great way to make your event memorable!


text:
Little Boy Blue Gingham Diaper Cake
Product Description
What better gift to give the parents of the new baby boy than a diaper cake! Loaded with diapers, which mom and dad will make great use of, this cake is a precious way to stock the baby's diaper bag. We also include baby accessories that every parent needs and put it all together and send it out beautifully wrapped with your gift card message attached. We throw in a personalized ribbon that we wrap around the cake that you can say whatever you wish, to welcome the new baby boy into the world!
This Diaper Cake Features:
- 35 Size 1 Disposable Diapers
- It's A Boy Stork Decoration
- Personalized Ribbon
- Super Soft Elephant Plush Toy
- Johnson's Baby Lotion, Baby Bath, Baby Powder, and Shampoo
- Blue Gingham Scratch Mittens
- Barbell Rattle
- Pacifier and Clip
- Fork and Spoon Set
- Desitin Diaper Cream
- 2 Burp Cloths
Additional Information
|Gift Basket Specialities
|Diaper Cake Specialities
3-Tier, Boy, Personalized
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18925 Stony Plain Road NW, Edmonton, AB T5S 2Y4
+ taxes & licensing
DEMO CAR, ORIGINAL PRICE OF $71,950. Premium Package, ConnectedDrive Services, advanced real time traffic information, Harman Kardon sound system, heated seats and steering wheel, rear view camera with surround view, navigation, satellite radio, USB integration, Bluetooth, heated rear seats, parking sensors, and more.
This BMW 535i xDrive has an efficient and powerful turbocharged 6 cylinder engine producing 300 HP with an 8 speed automatic transmission and full time intelligent all-wheel drive. Agile for its size, it feels elegant as it drives, and provides a sporty ride with great acceleration and passing ability. It has a sophisticated and high-tech interior with amazing standard features, built with quality materials, and has great safety ratings. The benchmark for mid-size sedans, the 5 Series has been a classic for years, and offers something for everyone. This car is in new condition, not previously owned, benefitting from a great amount of warranty and a huge discount. Fully inspected, we offer a variety of options for extending warranty coverage, and financing is available OAC. Listed price subject to applicable fees and taxes. We are an AMVIC licensed dealer.
For more info, to set up a test drive, or to get pre-approved credit, email firstname.lastname@example.com or call/text: Jason - 780 554 4337 or Levi - 780 884 5515
Please contact the seller to confirm pricing, features, odometer, and availability of this vehicle.
See the Carpages.ca Terms & Conditions for more details.


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parochial schoolnoun [ C ]uk /pəˈrəʊ.ki.əl ˌskuːl/ us /pəˈroʊ.ki.əl ˌskuːl/ US
› a school controlled by a religious organization that usually receives no money from the government教会学校
(Translation of "parochial school" from the Cambridge English-Chinese (Simplified) Dictionary © Cambridge University Press)
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the action of smoking a cigarette, pipe, etc., or the activity of doing this regularly


text:
Hawkrigg: CowardsMature
Silverbeak, let out a loud screech, which got my attention quite easily. He was known for his ear peircing screeches. He started flying into the forest, i didnt know why at first until i saw the branches move. I went into the forest after him.
There was two Dryads running through the trees with us in quick pursuit. I got out my bow, then one of them turned back, her violet eyes meeting mine. I could see fear in her eyes. I got out my bow, and shot her in the leg. She screamed in pain before falling onto the floor, grasping her leg. Silverbeak leaped onto her and the rest i didnt see as i had flew past him.
Now theres just one, who had teal coloured hair. She started to climb trees, and jump about. Nothing i couldnt keep up with, she didnt seem to be that good, shes acting as if shes never been in a fight before. I decide id mess about with her, as she is already scared but it is rather fun to rub it in.
I flew in front of her, making her swiftly change direction. I did this a few times, until she smacked me out of the way. I went into a tree, and she continued to run through the forest. Right then, no more games, im going to finish this.
I jumped back up, and flew straight into the air, and flew above her. At a point i deemed right i swooped down. Landing directly onto her and i took out my spear and went for the kill...


text:
This pack contains fully standalone trailer, it has advance clutch, custom wheels and can work with all DLC packs.
Credits: Stanley, SCS software, Rommi TZ
Compatibility: ETS2 v1.25
How to install mod for ETS 2:
- Download this mod.
- Extract it!
- Paste the .scs file(s) into your My Documents/Euro Truck Simulator 2/mod folder.
- Run game ~> Mod Manager ~> Enable mod.
- Enjoy!


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La bouche de Jean-Pierre
Lucile Hadzihalilovic
Excellent scope-camerawork and a great soundtrack characterise this lugubrious and slightly tasteless look at life in a stuffy apartment. After her mother has tried suicide, the young Mimi moves in temporarily with her Aunt Solange. There is also Solange's fiancé Jean-Pierre, who feels attracted to her.


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This is Stockholm
Modernity meets tranquility. Trend-setting restaurants and vibrant nightclubs next to idyllic archipelago. Welcome to a city where it is easy to breathe – and to feel your pulse.
The classic way of taking in the sights. And why not? A bus is a relaxing way of getting around Stockholm in comfort while your guide talks you through the highlights. Stay seated all the way or hop on and off as you fancy.Strömma offers a variety of tours including the Stockholm Panorama Tour, and the hop on hop off tours Open Top Tour and City Sightseeing Worldwide. For more details, visit Strömma's website.
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|P.J. Lashley and Bryan Cooksey.
JACKSONVILLE, Fla. — McCall Service, Jacksonville, Fla., announced the acquisition of Enviro Pest Control, also of Jacksonville. Enviro Pest Control will continue to operate independently, but will merge its service center.
Enviro Pest Control is a family business established in 1974 by owners P. J and David Lashley.
P. J and David Lashley built a legacy of customer-focused, high quality service that fits the core values of McCall Service, according to McCall Service President and CEO Bryan Cooksey. "P. J and her husband David Lashley worked very hard to develop outstanding employees and provide excellent service to their customers," Cooksey said. "Their emphasis on training and customer service complements McCall's goal to provide the best pest control service to our customers."
Visit www.mccallservice.com to learn more about McCall Service.