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SPCD Mahavidhyalaya
Uttar Pradesh
Total Students
Fees Range
How to reach
SPCD Mahavidhyalaya is situated at Devpalpur Choubepur,Kanpur Nagar in Kanpur.
Courses Offered
- 3 Years
- Bachelor of Law-LLB
- Politics / Law / Humanities
SPCD Mahavidhyalaya - SPCDM
College is feeling proud to tell all the guardians and students who are Financially weak and belongs to General, Minority, Schedule caste, OBC, Schedule tribe and unable to teach their children, Specially for those students who wants Education at Graduate and Post- Graduate which is under recognition of has got Affilation from Chartrapati Sahu Ji Maharaj(C.S.J.M.) University Kanpur . All the lecturers are highly qualified for the there job and fully devoted to there profession. This is our quality of Education providence. Mission of SPCDM is 'to foster critical analysis by training students both in theory and practice so as to equip them with skills needed for facing global challenges with team work and human values. And to build quality network with the Industry, educationists and the society for exchanging knowledge'. The modern teaching methodology assists the students in getting an all around view of engineering, technology and the entrepreneur world. The diligent faculty team of the college possesses hands-on experience and up to date knowledge of their subject and area of expertise. The infrastructure and facilities of SPCDM consists of spread-out campus alongside various amenities in the form of library, computer center, classrooms with proper teaching and learning aids, medical facilities etc. The institute seeks to place each of its students in an organization/company of great repute.
Why join SPCD Mahavidhyalaya
The college has an excellent infrastructure providing all the basic amenities.
1 Type Courses Available
- 1 UG Courses
SPCD Mahavidhyalaya Placements
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At SPCDM the students are endowed with modern infra and convenient amenities that make their life 'go easy' in college hours, alongside make learning more interesting. Some of the facilities include well ventilated classrooms, recreational areas, indoor and outdoor games, stocked library, seamless internet connectivity, medical facilities etc.
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1,000 BBK Graduation Party
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Branch: Elk River
Date: Thu Aug 21, 2014
Agegroups: Pre-schoolers
Time: 6:30 PM - 7:30 PM
Location: Meeting Room
By invitation only, for the last year's graduates of the Elk River 1,000 Books Before Kindergarten program. If you are interested in signing your preschooler up for this free, ongoing program sponsored by the Friends of the Elk River Library, please ask at the Elk River front desk.
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Central Basilica Hotel
Select your stay dates to check availability at Central Basilica Hotel
Central Basilica Hotel
About the Hotel
Property Location
With a stay at Central Basilica Hotel, you'll be centrally located in Budapest, steps from St. Stephen's Basilica and Andrassy Avenue. This 4-star hotel is within close proximity of Elizabeth Park and PANiQ-Room.
Make yourself at home in one of the 46 air-conditioned rooms featuring minibars and LCD televisions. Complimentary wireless Internet access keeps you connected, and satellite programming is available for your entertainment. Private bathrooms with shower/tub combinations feature complimentary toiletries and hair dryers. Conveniences include phones, as well as safes and desks.
Make use of convenient amenities such as complimentary wireless Internet access, a television in a common area, and tour/ticket assistance. Getting to nearby attractions is a breeze with the area shuttle (surcharge).
Buffet breakfasts are available daily for a fee.
Business, Other Amenities
Featured amenities include a 24-hour front desk, multilingual staff, and luggage storage. This hotel has 2 meeting rooms available for events. A roundtrip airport shuttle is provided for a surcharge (available 24 hours), and self parking (subject to charges) is available onsite.
Hotel Facilities
- Smoke-free property
- Year Built 1900
- Safe-deposit box at front desk
- Total number of rooms - 46
- Luggage storage
- Multilingual staff
- Elevator/lift
- Number of meeting rooms - 2
- Area shuttle (surcharge)
- Self parking (surcharge)
- Airport transportation (surcharge)
- Breakfast available (surcharge)
- Number of floors - 4
- 24-hour front desk
- Number of buildings/towers - 1
- Tours/ticket assistance
- Free WiFi
- Television in common areas
Room Facilities
- Phone
- Free toiletries
- Free cribs/infant beds
- Blackout drapes/curtains
- Iron/ironing board (on request)
- In-room safe
- Shower/tub combination
- Free WiFi
- Rollaway/extra beds (surcharge)
- Private bathroom
- Satellite TV service
- Air conditioning
- Desk
- Minibar
- Coffee/tea maker
- Daily housekeeping
- Hair dryer
- In-room climate control (air conditioning)
Hotel Policy
Know Before You Go
- This property offers transfers from the airport (surcharges may apply). Guests must contact the property with arrival details 24 hours prior to arrival, using the contact information on the booking confirmation.
- A resort fee is included in the total price displayed.
- Up to 3 children 8 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.
- Only registered guests are allowed in the guestrooms.
The following fees and deposits are charged by the property at time of service, check-in, or check-out.
- Breakfast fee: between HUF 3000 and HUF 3200 for adults, HUF 3000 and HUF 3200 for children (approximately)
- Airport shuttle fee: HUF 8500 per person (one way)
- Self parking fee: HUF 9500 per day
- Rollaway bed fee: HUF 9300 per night
The above list may not be comprehensive. Fees and deposits may not include tax and are subject to change.
Mandatory Fees and Taxes
The charges below are included in your overall room price:
- Resort fee
We have included all charges provided to us by the property. However, charges can vary, for example, based on length of stay or the room you book.
Renovations and Closures
This property is undergoing renovations from 01 November 2016 to 01 January 2017 (completion date subject to change).


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Welcome to Vela Dental Centers Kingsville online! Our dentists, Dr. Benjamin Vela and Dr. Alan Rodriguez, are excited to provide dental care for patients from age six and up. Our dentists and team are committed to providing you and your family with not just excellent dental services, but excellent customer service. Come to our office for all of your general and family dentistry needs. Our dental office offers a large range of dental services, so we rarely refer patients out. You can receive all of your dental care needs in one convenient location. Our friendly, experienced dental team is here to serve you and to make you feel relaxed and comfortable during your visit. We can accommodate the needs of Spanish-speaking patients.
Our state-of-the-art office makes use of the latest in dental technologies and techniques. Our dentists provide dental implants, replacement teeth that will become a permanent part of your smile. We also offer ClearCorrect™ and Six Month Smiles® for those patients who wish to straighten their smile. The use of digital imaging and laser dentistry helps our dentists to work with greater efficiency and accuracy.
Dr. Vela and Dr. Rodriguez have happily provided dentistry in Kingsville, Texas, and the nearby communities of Bishop, Premont, Riviera, Falfurrias, Sarita, Alice, Robstown, Banquete, San Diego, and Benavides, Texas, since 2009.
To learn more and to schedule an appointment, contact our dental office today.


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Nag Devta Temple is a prominent religious centre located 6 km away from Mussoorie, on Cart Mackenzie Road. A large number of devotees visit this temple every year to offer their prayers to the Hindu Lord of Snakes. The temple is most crowded on the eve of the Nag Panchmi, a major Hindu festival.
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Interesting take on the tumbling circulation figures of The Sun and the Daily Mirror by analyst Douglas McCabe, of Enders Analysis. The Sun fell below 3m for the first time since 1974 while the Mirror slipped below 1.5m for the first time since 1940.
He told Ben Fenton of the Financial Times that sooner rather than later one title will have to take the plunge and become a free newspaper. "These are psychological barriers," he said, "but I think they accelerate the arrival of the day when one of these two papers - probably The Sun - will decide to go free."
I don't rule it out, but it must be a long way off for The Sun. Publishing 3m full-colour papers every day and distributing them across the UK would be impossible to fund through current levels of advertising. I doubt that the Mirror's owners, Trinity Mirror, are contemplating such a move either.
But McCabe raised another matter that I overlooked in my analysis yesterday - the increasingly dramatic circulation situation for The Sun's Sunday sister, the News of the World. Last month it sold 6.31% fewer copies than in December 2006. With sales down to 3,167,435, it's only a matter of time - probably in the second half of this year - that the paper follows The Sun by falling below 3m.
That will be a significant moment, but it is unsurprising. There has been a consistent downward sales trend for all the red-top Sundays. None of them have found a successful way to engage readers in the past 25 years since their daily stablemates stole their agendas by running much more salacious and celebrity-driven material. The Sunday pops simply lost their USP.
As McCabe rightly said: "The fall in The Sun is more about people deciding, 'I don't need to buy this six days a week', but it is more worrying for the News of the World where people are taking the decision not to buy it on the only day it appears."


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We love Colorado. Always have. John and my families both vacationed there when we were growing up, and we honeymooned in Breckenridge for two weeks after our wedding in 2010. The whole state has a special place in our hearts. Two years ago we made a trip to visit friends ... Read more →
Pomegranate Lime Spritzers
This time last year this blog didn't exist. I was working at a coffee shop in a city I despised, John was only halfway through law school - which he hated - and 'our life' as we'd planned it just wasn't where we'd hoped. The funny thing is, our circumstances were all a result of ... Read more →


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Grace: The Degas
Grace smiled, disregarding her boredom as her 'friend' talked about her new shoes. Shoes were good, but when the conversation for half an hour was based on them, it made her want to rid the world of high heels, pumps, wedges, sandles, in fact, any of the shoes which Amber Rose Taylor adored. God the girl could talk!
"Amber darling, I have to go. I have a meeting. See you tomorrow?"
"Yes darling, chow." Amber kissed Grace's cheek and then continued her conversation. Grace rolled her eyes as she walked away. She only put up with Amber because she was the daughter of one her late mother's family friends.
She slid in to her car, slipping off her high heels and throwing them on the seat next to her. Thank god that was over.
The engine purred in to life; an Aston Martin VH Vantage Roadster in cherry red that had caught Grace's eye as she drove past the dealership late last month. It was her. So she'd bought it.
She drove home, and wasted no time in collapsing on the sofa. She closed her eyes.
Bring bring! Bring bring!
"Ahhhhhh! Never a minute alone!" She grabbed the phone."Hello?"
"Grace, you're still coming to the art opening tonight right?"
"Yes Alex, I'm still coming."
"I'm nervous."
"You've worked on this for years, don't be."
"Alright, look I gotta go. See you at eight."
Alex, her ex, owned a large art gallery that had just been re-opened. Tonight was the grand opening. Grace slipped off the sofa and in to her bedroom, opening up the wardrobe. She took out the black cocktail dress, red belt and red wedges she had gotten for the evening and laid them on the bed. She then went to shower.
As she wandered around in her underwear, her eyes kept slipping to the picture on the wall. Perhaps just a sneak peek before getting ready? She slipped her fingers underneath the frame and pulled at the small lever. She heard the clunk at the back of the wardrobe, which is found just next to the picture, and entered the wardrobe. she pushed aside the panel at the back and was confronted by the black leather catsuit, gloves and mask. She stroked the material. It was a pity she would not be able to slip it on tonight. With a sigh she closed the panel again and locked it.
"Alex, the place looks fantastic! In fact, so do you." Grace smiled, a glass of champagne in one hand.
"Thank you. These heels are killing me." Alex looked down at her feet. "You look great."
Grace twirled.
"Thanks. Looks like you're going to make another sale." Grace pointed, and Alex dashed off.
Grace wandered round, reviewing the work. Nothing really jumped out at her. Not even the very expensive, original Degas. It wasn't her favourite of his works. She was just going to head over to Alex again, when suddenly the lights went out. There were a few little shrieks and people scurrying about. And then the lights returned.
The Degas was gone. And on the wall, was a note.
Thanks for the Degas! It'll look great on my wall.
Grace narrowed her eyes. Snake Eyes?


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The mission of the Historical League, Inc. is to support the Arizona Historical Society and the AZ Heritage Center at Papago Park, Tempe, Arizona, formerly the Arizona Historical Society Museum, by contributing the services of its members, securing and allocating resources, and conducting informational and promotional activities.
Please join us by thanking the many volunteers of the Historical League at our at our annual Recognition Luncheon. Tuesday May 9th at McCormick Ranch Golf Club Pavilion with guest speaker Jana Bommersbach. Open to the Public.
View Events
If you are someone who loves Arizona and its history, joining the Historical League may be right for you. Our organization is very social in nature and we feature lectures from historical experts at our monthly meeting, held the first Monday of each month. In addition, our members travel to historic sites and museums all over the state, as well as out-of-state trips to visit historic sites.
The Arizona Historical Society is Arizona's oldest cultural organization, founded by the Arizona territorial legislature in 1864. The Historical Society is responsible for preserving Arizona's rich and dynamic history, both for present and future generations. The centerpiece of the Society in Central Arizona is the AZ Heritage Center in Papago Park; 1300 N. College Ave, Tempe, AZ 85281. This magnificent tribute to Arizona's history is a Smithsonian affiliate. The Mission of the Historical League is to support the AZ Heritage Center in Papago Park. http://www.arizonahistoricalsociety.org/museums/tempe/
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Imaging and imagination: understanding the endo-lysosomal system
- First Online:
- Accepted:
DOI: 10.1007/s00418-008-0384-0
- Cite this article as:
- van Meel, E. & Klumperman, J. Histochem Cell Biol (2008) 129: 253. doi:10.1007/s00418-008-0384-0
- Citations
- 1k Downloads
Lysosomes are specialized compartments for the degradation of endocytosed and intracellular material and essential regulators of cellular homeostasis. The importance of lysosomes is illustrated by the rapidly growing number of human disorders related to a defect in lysosomal functioning. Here, we review current insights in the mechanisms of lysosome biogenesis and protein sorting within the endo-lysosomal system. We present increasing evidence for the existence of parallel pathways for the delivery of newly synthesized lysosomal proteins directly from the trans-Golgi network (TGN) to the endo-lysosomal system. These pathways are either dependent or independent of mannose 6-phosphate receptors and likely involve multiple exits for lysosomal proteins from the TGN. In addition, we discuss the different endosomal intermediates and subdomains that are involved in sorting of endocytosed cargo. Throughout our review, we highlight some examples in the literature showing how imaging, especially electron microscopy, has made major contributions to our understanding of the endo-lysosomal system today.
KeywordsElectron microscopy Endosomes Imaging Lysosomal membrane proteins Lysosomes Mannose 6-phosphate receptor Protein sorting trans-Golgi network
Lysosomes are the terminal degradative compartments of the endocytic pathway. They receive extracellular components via endocytosis and intracellular material via autophagy, as well as via the biosynthetic pathway (Eskelinen 2005; Klionsky 2007; Luzio et al. 2007; Sachse et al. 2002a). By modulating the degradation of a multitude of proteins, lysosomes are key organelles in the regulation of cellular homeostasis. Moreover, these catabolic functions are complemented by a group of cell type-specific lysosome-related organelles, which store and/or secrete key factors involved in a diversity of functions, such as blood clotting, antigen presentation, pigmentation and alveolar surface tension regulation (dell'Angelica et al. 2000a; Raposo et al. 2007; Weaver et al. 2002). Because of their many functions, defects in lysosome functioning can have devastating consequences, as is illustrated by the rapidly growing number of human disorders displaying a primary or secondary defect in the lysosomal system. To date, lysosomal aberrations have been found in the 'classical' lysosomal storage disorders, as well as in muscular and neurological disorders, numerous types of cancer, immune-deficiency diseases and pigmentation-bleeding disorders (Futerman and van Meer 2004; Kroemer and Jäättelä 2005; Mohamed and Sloane 2006; Nixon 2005; Saftig et al. 2001).
Unraveling the intrinsic complexity of the endo-lysosomal system is a major challenge for cell biologists. Here, we will review some of the current insights in the mechanisms involved in lysosome biogenesis and functioning. Imaging of the endo-lysosomal system combined with imaginative visions has been instrumental for furthering the conceptual understanding of the endo-lysosomal system. Therefore, on the special occasion of the 50th anniversary of the Journal of Histochemistry and Cell Biology, we will also highlight some examples in the literature showing how imaging, especially electron microscopy (EM), has contributed to our understanding of the endo-lysosomal system today.
The discovery of the lysosome
The famous story of the discovery of the lysosome reads as a compelling detective novel (Bainton 1981; de Duve 2005). A major question that was faced by the cell biological society of the fifties was how cells could exert proteolytic activity without being digested by autolysis. The first indications on the existence of lysosomes came from studies designed to answer an entirely different question-i.e. to clarify the action of insulin on the liver-in which acid phosphatase activity (nowadays known as a lysosomal activity) measurements were used as a control. A combination of experiments from the labs of Christian de Duve and Albert Claude led to the intriguing observation that acid phosphatase in fresh fractions, prepared by a relatively gentle protocol, showed astonishingly low activity levels. By contrast, samples that were obtained by a crude fractionation protocol or stored for some days before assaying showed elevated levels of phosphatase activity. When this same hide-and-seek strategy was found for four additional hydrolytic enzymes, all with an acidic pH optimum and the same sedimentation characteristics as acid phosphatase, de Duve concluded that these enzymes must be contained together in particles surrounded by a membrane 'to prevent the enzymes getting out and the substrate getting in'. The term lysosome, for lytic particle or digestive body, was introduced (de Duve et al. 1955).
Another major clue was provided by Werner Straus. He found that 'droplets' of unknown function within the proximal tubules of the kidney contained reabsorbed (i.e. endocytosed) material as well as acid phosphatase activity and other lysosomal hydrolases (Straus 1954, 1956). This observation provided the first link between lysosomal digestion and endocytic uptake of extracellular material. Hence, the concept was born of the lysosome as a membrane-bound organelle that contains acid hydrolases involved in the digestion of substances that enter the cell via endocytosis (de Duve 1959). It explained that cells digest without autolysis by setting apart the degradative enzymes together with their endocytosed substrates in a membrane-bound compartment. The answer to the riddle was found.
The endocytic pathway in a nutshell
Defining early and late endosomes
When studying the endo-lysosomal system, one is forced to continuously pose the question how to discriminate between the distinct endosomal intermediates. The distinction 'early' and 'late' endosomes often has too limited discriminating power to describe the dynamic continuum of the endo-lysosomal pathway. In a recent EM study (Mari et al. 2007), a novel method to distinguish early and late endosomes was introduced by combining morphological, kinetic and molecular criteria. Early endosomes are by definition accessible to internalized transferrin (Tf) that recycles to the plasma membrane. As a result, late endosomes contain only marginal levels of Tf. With this in mind, the number of ILVs within the endosomal vacuoles was correlated with the occurrence of internalized Tf. Tf-positive early endosomal vacuoles on average contained one to eight ILVs, whereas endosomal vacuoles with nine or more ILVs were mostly devoid of Tf and therefore designated as late endosomes. A similar analysis with various other established early endosomal marker proteins corroborated and extended this definition. For example, the early endosomal antigen 1 (EEA1) appeared to be connected with only the very early maturation stages of early endosomes (one to five ILVs), whereas Rab11 appeared only on the later maturation stages of early endosomes (four ILVs or more). The early endosomal markers Rab4 and Hrs were more evenly distributed. Although the interpretation of this study is currently limited to the cell line in which the analysis was performed, i.e. the human hepatoma cell line HepG2, these observations illustrate that distinct stages of endosomes can be better monitored with increasing means of analysis.
TGN-to-endosome transport pathways
To fulfill their degradative function, lysosomes must also receive lysosomal proteins. These are synthesized in the endoplasmic reticulum and Golgi complex, after which they enter the TGN (Fig. 3a). From the TGN, multiple pathways to the endosomal system arise, mediating delivery of essential components to various endosomal intermediates. Thus, lysosomes receive their components via both the endocytic pathway and biosynthetic pathway.
Two classes of proteins together are essential for lysosomal functioning: the soluble lysosomal hydrolases and lysosomal membrane proteins. Each lysosomal hydrolase targets specific substrates for degradation and presently more than 50 different types are known. The heavily glycosylated lysosomal membrane proteins encompass a group of proteins with various functions. The major lysosomal membrane proteins (Marsh et al. 1987) are the lysosome-associated membrane proteins (LAMP)-1 and -2, the lysosomal integral membrane protein (LIMP)-2 and the tetraspanin CD63 [originally identified as platelet-activating glycoprotein (Pltgp40) and also often referred to as melanoma-associated antigen ME491, LIMP-1 or LAMP-3]. Initially, lysosomal membrane proteins were thought to mainly play a role in lysosomal stability and integrity, but currently new functions emerge involving chaperone-mediated autophagy and macroautophagy (reviewed by Eskelinen et al. 2003). Thus, the proper targeting of lysosomal proteins from the TGN to the endo-lysosomal system is an essential process in the biogenesis and maintenance of lysosomes. The best-known pathway for TGN-to-endosome transport is the delivery of soluble lysosomal hydrolases by the 300 kD cation-independent (CI) and 46 kD cation-dependent (CD) mannose 6-phosphate receptors (MPR) (Kornfeld and Mellman 1989; von Figura 1991). However, as will be outlined in the next paragraphs, increasing evidence indicates the existence of multiple additional or alternative pathways from the TGN to endosomes.
MPR-dependent transport of soluble lysosomal hydrolases
Both the CI and CD-MPR are present in almost all mammalian cell types. The lumenal domain of the CI-MPR is composed of 15 repetitive units, with two mannose 6-phosphate (M6P)-binding sites, whereas the CD-MPR has a lumenal domain of 159 amino acids, resembling one such unit, and has a single M6P-binding site. The M6P-binding sites recognize M6P-moieties, which are specifically present on the precursor forms of newly synthesized lysosomal hydrolases. Both receptors predominantly function as non-covalently bound homodimers. A specific role for each MPR in the targeting of lysosomal hydrolases has not been established, but distinct lysosomal hydrolases can exhibit different affinities for CI or CD-MPR (Qian et al. 2008; Sleat and Lobel 1997). CI-MPR can endocytose extracellular lysosomal hydrolases, whereas under physiological conditions CD-MPR is not involved in endocytosis. CD-MPR shows enhanced ligand binding in the presence of divalent cations (Hoflack and Kornfeld 1985a, b). Interestingly, the CI-MPR also has several other functions; it regulates the levels of circulating insulin-like growth factor II by binding it at the plasma membrane for delivery to lysosomes. Furthermore, it facilitates the activation of the precursor of transforming growth factor-β1, which is a growth inhibitor, and also binds retinoic acid, thereby mediating retinoic acid-induced apoptosis and growth inhibition, further supporting the idea that CI-MPR could be a tumor suppressor (reviewed by Ghosh et al. 2003a).
MPR-independent transport of soluble lysosomal hydrolases
In addition to the well-characterized MPR pathway, several additional transport pathways for lysosomal hydrolases exist (Fig. 3c). For example, the lysosomal hydrolases lysosomal acid phosphatase and β-glucocerebrosidase do not acquire M6P residues and their transport is not depending on MPRs (Aerts et al. 1988; Waheed et al. 1988).
Lysosomal acid phosphatase is a type I transmembrane protein that follows the constitutive secretory pathway to the plasma membrane and reaches the lysosome via endocytosis. In lysosomes, the transmembrane precursor is processed into a soluble form, but both proteins are enzymatically active (Waheed et al. 1988). Lysosomal acid phosphatase contains the tyrosine-based motif YRHV in its cytosolic domain, which is required for endocytosis, but does not mediate direct TGN-to-endosome targeting (Obermüller et al. 2002; Peters et al. 1990; Pohlmann et al. 1988).
β-Glucocerebrosidase has no membrane spanning domain (Erickson et al. 1985). After synthesis, it is glycosylated upon which it becomes membrane-associated (Rijnboutt et al. 1991). The machinery required for proper transport of β-glucocerebrosidase was long unknown. Recently, Paul Saftig and colleagues unexpectedly identified the lysosomal membrane protein LIMP-2 as a specific receptor for β-glucocerebrosidase. LIMP-2 binds β-glucocerebrosidase in a pH-dependent manner, enabling association in the ER and guiding it all the way to the lysosome, where the complex likely dissociates because of the acidic pH (Reczek et al. 2007). Whether LIMP-2 recycles between endosomes and TGN is not known. This study for the first time implies a lysosomal membrane protein in the transport of a lysosomal hydrolase. It will be very interesting to establish whether LIMP-2 acts as a more general binding partner for lysosomal hydrolases. In addition, characterization of the molecular machinery required for transport will provide further insight into this TGN-to-endosome transport pathway.
Lysosomal hydrolases that are normally modified with a M6P residue can also traffic in an MPR-independent way to the lysosomes (Fig. 3c). This became apparent by comparing different cell types from patients with I-cell disease, also known as Mucolipidosis type II. In this disease, soluble lysosomal hydrolases do not acquire M6P groups due to a deficient activity of the enzyme GlcNAc-phosphotransferase (Hasilik et al. 1981; Reitman et al. 1981). As a consequence, the lysosomal hydrolases follow the default constitutive pathway to the plasma membrane (Fig. 3c) and are thus secreted. However, in cells of non-mesenchymal origin, most lysosomal hydrolases are still targeted correctly to the lysosome (Fig. 5) (Little et al. 1987; Owada and Neufeld 1982; Waheed et al. 1982), suggesting the existence of alternative pathways. The same phenomenon was observed in a very recently established mouse model system for I-cell disease, achieved by GlcNAc-phosphotransferase knockout (Gelfman et al. 2007). Furthermore, in mice that are deficient of both MPRs, the intracellular activities of several lysosomal hydrolases are comparable to the control situation (Dittmer et al. 1999). These findings indicate that lysosomal hydrolases can follow a pathway to the lysosome that is not mediated by MPRs.
The mechanisms and pathways of MPR-independent transport are still poorly defined. Recent studies, however, have implicated a role for the multiligand receptor sortilin as an alternative receptor for a subset of lysosomal proteins, called sphingolipid activator proteins (SAPs), which are non-enzymatic cofactors required for the degradation of glycosphingolipids. Two SAPs are known; the precursor protein prosaposin, which renders saposin A, B, C and D after proteolysis, and GM2 activator protein (AP), an essential cofactor for β-hexosaminidase A. Studies from Lefrancois and colleagues have shown that sortilin interacts with both prosaposin and GM2AP, which is independent of M6P tags, whereas depletion of sortilin induced their secretion (Lefrancois et al. 2003). It remains to be established, however, whether sortilin is the exclusive receptor for SAPs, or whether MPR has an additional role in their transport. Interestingly, sortilin, together with SorLA and SorCS1–3, forms a protein family sharing homology to the lumenal domain of the yeast vacuolar sorting protein Vps10p, which directs carboxypeptidase Y to the vacuole (Marcusson et al. 1994). Immuno-localization studies positioned sortilin predominantly in endosomes and the TGN (Mari et al. 2007), where it colocalized with CI-MPR in AP-1 and GGA3 positive clathrin-coated transport carriers, indicating that sortilin and MPRs travel via the same carriers to the endo-lysosomal system. Currently, the functions of the SorCS-subgroup are unknown, but since SorCS3 is predominantly localized at the plasma membrane it is unlikely to be involved in TGN-to-endosome trafficking (Westergaard et al. 2005). Whether sortilin or possibly SorLA could act as alternative receptors for lysosomal hydrolases is still unclear.
TGN-to-lysosome transport of lysosomal membrane proteins
Lysosomal membrane proteins that exit the TGN can be transported to lysosomes following either a direct or indirect pathway, involving passage over the plasma membrane. Virtually nothing is known about the carriers and molecular machinery that mediate the direct route of lysosomal membrane proteins to the lysosomes. Moreover, the contribution of this pathway remains debated (Carlsson and Fukuda 1992; Janvier and Bonifacino 2005). Important for the sorting of lysosomal membrane proteins to lysosomes are the tyrosine and dileucine-based consensus motifs within their cytosolic tails. LAMP-1, LAMP-2 and CD63 (LIMP-1) all bear a YXXØ motif, with an essential tyrosine residue, hydrophobic XX residues and the Ø residue being a bulky hydrophobic amino acid (reviewed by Bonifacino and Traub 2003). The YXXØ motif is required for endocytosis at the plasma membrane, but has also been implicated in the direct targeting from TGN to lysosomes. For TGN sorting, however, additional characteristics seem to be required; a glycine residue preceding the critical tyrosine, the XX residues being acidic and the entire motif positioned six to nine residues from the transmembrane domain.
LIMP-2, the recently identified receptor for β-glucocerebrosidase, has a dileucine-based sorting motif DXXLL, also referred to as acidic cluster-dileucine motif, which has also been implicated in both internalization and TGN-to-lysosome targeting. The YXXØ motifs are recognized by the μ subunits of AP-1, AP-2, AP-3 and AP-4 and the DXXLL motif by the VHS domain of the GGAs (reviewed by Bonifacino and Traub 2003). The role of adaptor proteins in TGN sorting of lysosomal membrane proteins remains to be established. Segregation of LAMP-1 into AP-1 positive vesicles was observed in a biochemical study and also confirmed by immuno-EM, indicating exit from the TGN in clathrin-coated vesicles (Höning et al. 1996; Hunziker and Geuze 1996). However, sorting of LAMP-1 is not strictly dependent on AP-1. In mice lacking the μ1A subunit, which impairs AP-1 binding and clathrin assembly at the TGN, there was no significant increase in plasma membrane levels of LAMP-1 (Meyer et al. 2000), whereas the protein was still found in lysosomes. Depletion of clathrin in HeLa cells did result in increased levels of LAMPs at the cell surface, but not a complete redistribution of LAMPs to the plasma membrane (Janvier and Bonifacino 2005), indicating the existence of a direct TGN-to-lysosome pathway independent of clathrin (Karlsson and Carlsson 1998). Interestingly, depletion of AP-1 in HeLa cells, like in mice, again had only modest effects on LAMP transport over the surface (Janvier and Bonifacino 2005). Together, these data indicate that in addition to AP-1 and clathrin, other factors might be involved in direct TGN-to-lysosome transport of LAMPs.
The presence of a clathrin-independent TGN-to-endosome pathway was also indicated by studies on cells that present antigens, like B lymphocytes, macrophages and dendritic cells, in the context of major histocompatibility complex (MHC) class II molecules (Fig. 3c). The newly synthesized MHC II molecules are transported from the TGN either via the plasma membrane or directly to so-called MHC II loading compartments (MIICs) that are lysosome-related organelles representing early and late endosomes in these cells (Kleijmeer et al. 1997). For proper transport of the integral membrane protein MHC II from TGN to MIICs, association with the type 2 transmembrane protein, invariant chain (Cresswell 1996), is important. Invariant chain contains [DE]XXX[LI]-type signals in its C-terminus, but also the cytoplasmic tail of class II was found to contain endosomal sorting information (Bakke and Nordeng 1999; Bonifacino and Traub 2003). The TGN-to-endosome pathway of MHC II/invariant chain was studied in detail in B lymphoblasts by a combined biochemical and immuno-EM approach (Glickman et al. 1996). This revealed that MHC II, together with cathepsin D, in both normal and I-cell disease B lymphoblasts entered TGN exit domains that did not contain AP-1 or clathrin and were devoid of CD-MPR. Further studies are required to establish the molecular machinery of this pathway.
Interestingly, a recent study linked the TGN exit of lysosomal membrane proteins to GGA3, the same component involved in the exit of MPRs. GGAs are monoubiquitinated at their GAT (GGA and TOM) domain, which supports the idea that ubiquitin is a universal sorting signal, acting at various intracellular sites. The lysosomal-associated protein transmembrane-5 (LAPTM5), which is specifically expressed in hematopoietic cells, associates in the TGN with both the ubiquitin ligase Nedd4 and GGA3 (Pak et al. 2006). This association is required for efficient targeting of LAPTM5 to lysosomes and indicates that ubiquitination of GGA3 is possibly accomplished by Nedd4. The finding that GGAs can be involved in TGN-to-lysosome transport of a lysosomal membrane protein might explain the mild effect of AP-1 knockdown. Clearly, further studies are required to clarify this direct TGN-to-endosome transport pathway of lysosomal membrane proteins.
The early endosome: from CURL to tubular sorting endosome
The combined data on TGN-to-endosome transport of lysosomal hydrolases and lysosomal membrane proteins suggest the existence of multiple TGN exits for direct delivery to the endosomal system (Fig. 3c). There may be several reasons for such different pathways. For example, they allow custom tailored regulation of the delivery of distinct types of cargoes and provide a back-up system in case a given pathway is no longer available (such as in I-cell disease). The existence of alternative TGN-to-endosome pathways would also allow different cargoes to enter the endo-lysosomal system at distinct stages, which may coincide with their function and mode of sorting. In addition to understanding TGN exits, it is therefore equally important to define the recipient endo-lysosomal intermediates in lysosome biogenesis. With the refining of EM techniques, our understanding of the endo-lysosomal system has equally evolved. The technique that we now know as 'classical' EM, i.e. thin sections of plastic-embedded samples of chemically fixed cells, allowed our first acquaintance with the fascinating complexity of the cell's inner world. Immunocytochemistry added another dimension by allowing the localization of enzymatic activities to specific compartments (as illustrated for acid phosphatase). The next step was the development of immunocytochemical procedures, which greatly increased the repertoire of proteins that can be visualized in the cell.
Over the years, it became clear that CURL was involved in the sorting of multiple types of proteins and the modern structural equivalent of CURL is the early endosome. Indeed, the early endosome is the major cellular site, where cargo destined for recycling is sorted from cargo en route to late endosomes and lysosomes. To indicate this, early endosomes are often subdivided into the globular 'vacuolar or sorting' endosome (Fig. 3b), whereas the associated tubules were indicated as 'recycling tubules' or 'recycling endosomes'. However, recent studies provided accumulating evidence that early endosome-associated tubules are more than just transport intermediates for recycling cargo; they are also actively involved in sorting (Fig. 3b).
With the increasing number of antibodies against distinct types of cargoes and by optimizing immuno-EM techniques (Slot and Geuze 2007), it became possible to localize less abundant cargoes. This revealed that at steady state conditions minor amounts of LAMP-1 and LAMP-2 are found in the early endosome-associated tubules (Fig. 6, right panel). The importance of this localization became apparent from studies on the pigmentation-bleeding disorder Hermansky-Pudlak-syndrome-2 (HPS-2). Cells from HPS-2 patients lack a functional AP-3 adaptor complex and show an increased transport of lysosomal membrane proteins over the plasma membrane (dell'Angelica et al. 1999). A combination of biochemical and immuno-EM approaches revealed that AP-3 mediates the exit of lysosomal membrane proteins from the early endosome-associated tubules, most likely for transport to late endosomes or lysosomes (dell'Angelica et al. 1998; Peden et al. 2004) (Fig. 3b). Impairment of AP-3 function results in an increased cycling of lysosomal membrane proteins between early endosomes and plasma membrane (Peden et al. 2004), which explains the increased levels of lysosomal membrane proteins at the plasma membrane of HPS-2 cells. Importantly, early endosomes can be reached by lysosomal membrane proteins via the plasma membrane, but possibly also via a direct pathway from the TGN. This may explain why in the literature AP-3 has been implicated in the direct as well as indirect pathway to lysosomes (Ihrke et al. 2004; Rous et al. 2002). In melanocytes, AP-3 also mediates a pathway from early endosomes to melanosomes, which are lysosome-related organelles (Theos et al. 2005).
Importantly, by multiple double- and triple immunogold labellings, it was shown that a particular early endosome-associated tubule could contain the recycling proteins Tf and ASGPR, the CD and CI-MPR, and LAMP-1 and LAMP-2. In addition, one single tubule could display multiple budding profiles that were positive for either the adaptor complex AP-1 or AP-3 (Peden et al. 2004). AP-1 on endosomal tubules has been implicated in the retrograde transport of the CD-MPR and of the Shiga toxin B subunit to the TGN (Meyer et al. 2000), as well as recycling of internalized Tf (Mallard et al. 1998; van Dam and Stoorvogel 2002). The presence of distinct types of recycling proteins and the association of multiple adaptor complexes indicated that the early endosome-associated tubules mediate sorting of cargo proteins to distinct destinations in the cell, i.e. plasma membrane, TGN and late endosomes or lysosomes. Therefore, these AP-3 positive compartments were introduced as tubular sorting endosomes (Figs. 3b and 6, right panel) (Peden et al. 2004). A recent review of Bonifacino and Rojas extends this concept (indicated here as 'tubular endosomal network'), by ascribing all protein machinery currently known to be involved in endosome-to-TGN transport to distinct exits of the tubular sorting endosome (Bonifacino and Rojas 2006).
Together, the available data launch the concept of a two-step rocket. In the first step, at the vacuolar domain of early endosomes, proteins destined for late endosomes-lysosomes are sorted from recycling proteins that accumulate in the attached tubules. Then, in the second step, taking place in the tubular sorting endosome, recycling proteins are sorted out to distinct destinations in the cell. Within this model, the recycling endosomes are one type of transport intermediate that evolve from the tubular sorting endosomes, mediating the recycling of cargo to the plasma membrane (Fig. 3b).
Multiple sorting events at the early endosomal limiting membrane
Despite the relatively simple and attractive concept of the two-step rocket, sorting at the early endosomes displays another level of complexity; recycling carriers not only evolve from tubular sorting endosomes, but also from endosomal vacuoles. A recent study showed the formation of so-called endosome-to-TGN carriers (ETCs) from the limiting membrane of early endosomal vacuoles (Fig. 3b) (Mari et al. 2007). These ETCs are so far specifically involved in the endosome-to-TGN recycling of lysosomal protein receptors, i.e. the CD- and CI-MPRs and sortilin. Importantly, ETCs constitute an entirely different population of recycling carriers than those emanating from the tubular sorting endosome. With the exception of the MPRs, ETCs are devoid of any of the cargo or machinery proteins found in the tubular sorting endosome. The associated machinery proteins of ETCs are SNX1, SNX2 (Carlton et al. 2004, 2005; Mari et al. 2007) and hVps26p (Arighi et al. 2004; Mari, personal communication). ETCs also differ from tubular sorting endosomes by morphology. By 3D-tomographic analyses, ETCs were identified as non-branched, short tubules and vesicles, not organized in a reticulum.
Apart from segregating proteins into tubular sorting endosomes and ETCs, the limiting membrane of early endosomal vacuoles is involved in a third protein sorting event; i.e. segregation of proteins into a characteristic bilayered coated area that is involved in protein sorting into ILVs (Fig. 3b, see also below) (Bonifacino and Rojas 2006; Peden et al. 2004; Raiborg et al. 2002; Raposo et al. 2001; Sachse et al. 2002b). Thus, proteins present in early endosomal vacuoles may be sorted to at least three types of specialized sorting domains: tubular sorting endosomes, ETC or the bilayered coat. The formation of two types of exits from the non-coated regions of the early endosomal vacuoles predicts the existence of complementary molecular machineries that specifically recruit cargo to either of these recycling compartments (i.e. ETC or tubular sorting endosome). Future investigations are needed to further unveil the components of these machineries.
Sorting to late endosomes and lysosomes
The default pathway for molecules entering early endosomes is recycling back to the plasma membrane, a pathway that starts with the segregation of cargo into the tubular extensions of the early endosomes (Draye et al. 1988; Yamashiro et al. 1984). Endocytosed cargo from the plasma membrane that is not destined for recycling requires active sorting to late endosomes/lysosomes, a process mostly mediated by inclusion into ILVs (Gruenberg and Stenmark 2004; Katzmann et al. 2002; Sachse et al. 2002b).
Protein sorting into ILVs is critical for growth factors and their receptors, such as growth hormone (GH) (Sachse et al. 2002b) and epidermal growth factor (EGF) (Raiborg et al. 2003; Urbe et al. 2003). Sorting into ILVs segregates these ligand–receptor complexes from the cytoplasm, thereby terminating signaling. ILV sorting requires ubiquitination and involves among others the cytoplasmic proteins Hrs and STAM (Bache et al. 2003b; Raiborg et al. 2001, 2002; Urbe et al. 2003). By immuno-EM, it was shown that at the limiting membrane of the early endosomal vacuole the GHR and EGFR are concentrated in flat, bilayered coated areas with a fuzzy outer layer of clathrin and a dense inner layer of unknown protein composition. These bilayered coated domains were enriched in Hrs (Sachse et al. 2002b). These findings led to the proposition of the 'sorting-by-retention' model for endosomal sorting (Sachse et al. 2002b). This model describes that receptor–ligand complexes prior to their incorporation into ILVs are retained and concentrated in the bilayered coated areas of endosomal vacuoles, whereas recycling proteins pass through the vacuolar-limiting membrane and follow the bulk flow route towards recycling endosomes. A recent study showed that the recruitment of clathrin is responsible for concentrating Hrs in these endosomal microdomains, reinforcing an active role for clathrin in protein retention (Raiborg et al. 2006).
Downstream of Hrs, the targeting of ubiquitinated receptors into ILVs requires the 'endosomal sorting complexes required for transport' (ESCRT)-1, -2 and -3 in concert with Alix/AIP-4 and the AAA-ATPase (hVps4). hVps4 is required for disassembly and recycling of ESCRT complexes (Babst 2005; Bache et al. 2003a; Katzmann et al. 2003) and interestingly, also for the disassembly of the bilayered coat (Sachse et al. 2004). Impairment of hVps4p function gives rise to an elongated coat and decreased formation of ILVs, indicating that coat disassembly is required for the inward budding process to proceed (Sachse et al. 2004). Indeed, 3D-electron tomography of early endosomes showed that inward budding of ILVs is mostly seen at the edges of the coats (Murk et al. 2003). Notably, the sorting-by-retention model proposes a novel role for clathrin in addition to its role in the formation of transport vesicles, i.e. defining endosomal subdomains involved in protein retention.
Future developments
Another point of interest is that not all cargo might be recruited to ILVs by the same mechanism. Both in yeast (Bilodeau et al. 2002; Epple et al. 2003) and mammalian cells (Hislop et al. 2004), examples of cargoes are described that do not require direct ubiquitination. In case of the melanosomal protein Pmel17, which is also targeted to ILVs independently of ubiquitin and Hrs, two N-terminal lumenal subdomains seem necessary and sufficient for efficient transport (Theos et al. 2006). Possibly, these non-ubiquitinated cargoes associate with others for their association with the ESCRT machinery, but perhaps they use an alternative mechanism. Interesting in this respect is that LBPA can induce ILV formation in liposomes, in the absence of protein, suggesting that lipid-driven and ESCRT-dependent pathways may coexist (Matsuo et al. 2004). Also the lysosomal targeting of LAMP-1 and LAMP-2 remains an interesting open question, since these proteins are predominantly located on the limiting membranes of late endosomes and lysosomes, and incorporation into ILVs seems therefore not involved. By a more detailed characterization of the distinct endosomal maturation stages (Mari et al. 2007), it will be feasible to obtain a more accurate definition of the stage in which a specific cargo enters or exits the endo-lysosomal system. One of the questions that is currently addressed in our lab is whether lysosomal membrane proteins can travel directly to late endosomes or lysosomes, thereby bypassing the need of ESCRT sorting machinery.
Finally, with the development of live cell imaging methods and correlative live cell imaging EM, a unique tool is provided to integrate dynamic parameters with ultrastructural protein localization studies, which opens a whole new field of analysis.
We want to thank the scientists to whom we refer here for their often groundbreaking contributions to the understanding of the complexity and beauty of the endo-lysosomal system and apologize to those many others whose work we were not able to discuss. We also want to thank our colleagues of the Department of Cell Biology for the many scientific interactions and fruitful discussions. We express our special thanks to Marc van Peski for his help with preparation of the figures and to Maaike Pols, Muriel Mari and Hans Geuze for critically reading our manuscript. EvM and JK are supported by VICI grant 918.56.611 of the Netherlands Organization for Scientific research (NWO).
Open Access
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.


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Model Mayhem Admin
cstudioincPhotographer Male Hattiesburg, Mississippi, US
My MM URL: http://www.modelmayhem.com/cstudio
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About Me
I Have been in Photography for over 20 Years. You either like what you see or you don't. If you like what you see and are serious about what you do, contact me. If not, don't waste my time or yours. If you end up wasting my time, you don't get a second chance.
I have all the letters you use for the credentials and memberships to make sure I keep in check of what I am doing including PPA and SEP
I am full time photographer. Not a hobby. As a hobby I like to play tennis and shoot pictures that are fun for me.
My studio is capable of producing anything in the graphic world. including comp cards, business cards, and just about anything to get your career off the ground.
I serve a large variety of clients.
By this time you are bored or really want to know more. Do not hesitate to call the studio and ask for Lee. Let them know you found me through this site. They will let you talk to me. :-) Happy Shooting
Have you worked with cstudioinc? Add Credits for cstudioinc! >>
Credit Notes
Models worked with on this site:
Magazines I have worked for or been published in:
Teen Vogue - national
Rounder - national
excellence - national
Reps - national
Signature - local
Here's Hattiesburg - local
Mississippi Magazine - regional
If You are interested in our work, Please contact us. I will no longer be available for tfp/cd shoots in case it is a choice selection of my own or some monetary agreement is met. 601-450-0170
Thanks for viewing.
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EddieB85 Model
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MsHeidi Model
Kitty Gallanis Photographer
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June 18, 2014, Law360
David Kornblau is quoted regarding the U.S. Securities and Exchange Commission's administrative proceedings.


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Condition: Standard
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All Product Details
Henry James would want to study her.
Queen Victoria would recognize her as an Equal.
The New York Jets would want to have her on their side.
Lewis Carroll would love her (once he got over the initial shock).
She knows everything about The Plaza. She is interested in people when they are not boring.
She has Inner Resources.
If you take her home with you, you will always be glad you did.
-- Edward I. Koch (former NYC mayor!!!)
"Eloise is a model for uppity women."
-- Nina Totenberg (rawther important radio lady)
"Thanks to Eloise, my daughter sought independence and mastery over her life. We loved reading this book, and we've never given it away."
-- Susan Sarandon (rawther grande actress)
"Ooooooo....I just love Eloise! Thank you, Kay Thompson and Hilary Knight. You're the bestest!"
-- Judy Blume (lovely author)
"My first happy response to Eloise was entirely due to the brilliant, iconic images. That brazen, loose-limbed, deliciosly little girl monster is Hilary Knight at his best."
-- Maurice Sendak (enormously famous artist)
"Eloise, Kay, and Hilary have been in my life always. And her pug puppy is in my heart. What memories!"
-- Lena Horne (movie star)
The Eloise character grew out of the voice of a precocious six-year-old that Miss Thompson put on to amuse her friends. Collaborating with Hilary Knight on what was an immediate bestseller, Kay Thompson became a literary sensation when Eloise was published in 1955. The book has sold more than 2 million copies to date. Kay Thompson and Hilary Knight created four more Eloise books, Eloise in Paris, Eloise at Christmas, Eloise in Moscow, and Eloise Takes a Bawth.Hilary Knight, son of artist-writers Clayton Knight and Katharine Sturges, was educated at the Art Students League, where he studied with Reginald Marsh. Besides the Eloise books, Hilary Knight has illustrated more than fifty books for children, six of which he wrote himself.
He lives and works in New York City, not far from The Plaza Hotel.


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Les Péneyrals Campsite
Salignac, DordogneSite code: DR011
Parc Summary
- Whizz round 5 waterslides and enjoy the pools whatever the weather
- Try a spot of fishing at the lake in the middle of the parc
- Visit the spectacular medieval town of Sarlat, it's not far by car
What our customers say
Start your holiday...
Close to fortified Sarlat, with fishing on parc
Les Péneyrals is made for families who love to explore, but also want to enjoy their downtime poolside.
Close to the majesty of the Dordogne valley, this steeply-terraced parc wraps around a central lake but, not content with this, it also has its own large pool complex, comprising four pools (one heated with a retractable roof) and waterslides. The owners arrange a variety of traditional entertainment throughout the season, including live bands and weekly discos in high season. The parc enjoys plenty of shade from its mature planting and, as the sun sets, you could opt for a night exploring the bistros of nearby Sarlat, a handsome medieval town, or enjoy your parc's excellent evening entertainment.
Extras with Eurocamp
- Linen Hire
- Eurocamp Couriers on site
- Gas BBQ provided free of charge
- Welcome pack
- Games
General Information
Parc size
Small - 250 Pitches:
Opening Dates:
12/05/2017 - 10/09/2017
11/05/2018 - 09/09/2018
Nearest Airport:
Bergerac (EGC) - 87km
Recommended Port:
Caen - 648km
Direct Access to Lake:
Direct Access
Nearest Town:
Salignac - 5km
Pricing for Les Péneyrals Campsite
If you like this Parc you may also like...
St. Avit Loisirs CampsiteLe Bugue , Dordogne
Weave through French countryside, past medieval villages and you will find St Avit Loisirs, one of the country's premier campsites.Read more about this parc ›
Le Val de Bonnal CampsiteRougemont , Jura
How do you fancy a spot of wild swimming in beautiful countryside? Then get yourself to the heart of the Jura and stay at Le Val de Bonnal.Read more about this parc ›
Bella Italia CampsitePeschiera , Italian Lakes
On the south eastern tip of Lake Garda, at the start of the 'Olive Tree Riviera', lies Bella Italia, a beautiful, sprawling parc packed full of activities, making it the ideal choice for active families.Read more about this parc ›


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ED Magedson – Founder
Ripoff Report
enterprise rental8913 frankford ave philadelphia, Pennsylvania USA
enterprise rental SCAM!!! I DIDNT BUY THEIR INSURANCE SO THEY "FOUND" A DENT philadelphia Pennsylvania
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Rented a card from Enterprise while my car got worked on. Insurance was covering everything so I wasn't concerned...until i brought the car back...low and behold they "found" a dent after circling the car many times...they had my credit card because they say they need it with a drivers license..."dont worry, the insurance company is paying your rental" so why do you need my credit card?? now, they charged me 500.oo for a dent that was already there. All because they tried so hard to get me to pay for 150.00 insurance. Best part, they had the nerve to laugh at me on my way out the door! NEVER EVER EVER TRUST THIS COMPANY!!!!
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Photo by ISIPhotos.com
By DAN KARELL
The U.S. Women's National Team will be hosting Mexico in a pair of friendly matches as part of their final preparations for the 2014 CONCACAF Women's Championship, which will determine entry into the 2015 Women's World Cup.
While a venue for the first friendly on Sept. 13 has yet to be announced, the USWNT will return to Rochester for the sixth time and Sahlen Stadium for the fourth time when they take on Mexico on Sept. 18. The match will be televised on ESPN2 and WatchESPN.
Sahlen Stadium is the home of the Western NY Flash and Rochester is the hometown of USWNT forward Abby Wambach. Wambach and USWNT midfielder Carli Lloyd play their club soccer for the Flash.
The matches against Mexico will be the 29th and 30th in USWNT history. The U.S. is 26-1-1 against Mexico, and beat El Tri 7-0 last Sept. in a friendly match at RFK Stadium in Washington D.C. The USWNT is also undefeated in Rochester.
The pair of matches are the final warm-up matches ahead of the 2014 CONCACAF Women's Championship, which will take place between Oct. 15-26 in four venues – RFK Stadium, Sporting Park in Kansas City, Kan. Toyota Park in Bridgeview, Ill. and PPL Park in Chester, Pa.
Each team including the USWNT will play one game in each of the former three venues and the crucial semifinal and final matches will take place at PPL Park. The top three teams join hosts Canada at the 2015 Women's World Cup.
Up next for the USWNT is a friendly match on Aug. 20 against Switzerland from WakeMed Park in Cary, N.C.
What do you think of this news? Think this is the preparation for the CONCACAF Women's Championship? Should they have scheduled a European opponent or two?
Share your thoughts below.
So i thought maybe I'd died and was in heaven but I woke up and realized I was just dreaming I was married to Sydney LaRoux. How can she be so cute AND love soccer. If only I was 25 years younger 😦
Glad the game will be televised on ESPN2. Hope the Sep 13th match is somewhere on the west coast, though.
Mike R
Got that right Limey her and Alex Morgan,…great partnership stuff dreams are made of. Good to see them play Mex. Playing the US C team aka Mexico will be good practice
The Mexican Federation is a joke
The Mexican Mens national team play 90% of their games in the USA and the Mexican Women's National Team has 80% of players born in the USA
Why does mexico even have a team?
Ale Rojas Cortes
Wnt list : defenders( cox, Dunn, brunn, rampone, O'Hara, krieger, engen, Johnston, ) midfielders ( Lloyd, rapinoe, Brian, holiday, boxx , tymrak, Heath, o' Reilly, mewis ) forwards (wambach , Morgan, Sydney, press, Hagen, Rodríguez ) goalkeeper ( hope, Harris, neiher)
Very good players in the nwsl: GK: henninger , Barny . Molly pachman, Fletcher, Barnes, lepeilbet , kallman, mewis , dibernardo, hoy, winters, Reeve , friels Nick, Hayes, Edwards, ohai, bev.


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Chasing Sticky Icky 100x · see all
Lifetime fubar Rank: #338
Today's fubar Rank: #68
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Top Score: #493
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Chasing Sticky Icky 100x · see all
So here is a little bit about myself. Grew up in the big apple and moved to sunny Fla 1 year ago. Retired civil service..............wont say which one but if ya ever try to climb thru my window in the middle of the nite you ll find out quickly which 1. I keep a small FU Family and I help them as much as I can and I will help you as much as I can as long as I dont feel used. I do have a small bout of M. S. I know I say small and those who know of or have the disease know its not small but I am doing an amazing job with it since Dx in 2011 and considering I retired in 07 with a pension and dont have to break my ass to make a living it helps to maintain the disease. Im a die hard hairspray metal fan (those who are know what i mean). I do accept bling, help and give also to those who deserve so. I do have the gift of gab so feel free to say hi, get to know me a little and enjoy life. YOLO !!!!!!
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text: DEERFIELD, Ill., April 14, 2014 /PRNewswire/ -- Hornitos® Tequila is redefining the premium tequila experience with the launch of new Hornitos® Black Barrel®, a 100% blue agave tequila, distinctively aged through an innovative process that conveys the classic tasting notes of a whiskey. Triple aged to create a uniquely complex tequila with whiskey characteristics, the newest premium offering from Hornitos® is aged first for 12 months in American Oak barrels before transitioning into deep charred American Oak barrels for four months. In the final aging process, the liquid is transferred to specially-toasted American Oak barrels for two additional months to further enhance the whiskey-like notes of this one-of-a-kind premium tequila. The distinctive Hornitos® Black Barrel® aging process creates a spirit unlike anything else in the tequila category. With oaky-vanilla notes and a peppery spiciness, the tequila offers light smoky oak notes and smooth, sweet agave character. The spirit is full-bodied with a clean, mild flavor finish and a slightly lingering tingle sensation. Prior to hitting shelves, Hornitos® Black Barrel® quickly became an award-winning tequila with accolades at international spirits competitions. Hornitos® Black Barrel® won the 2014 Best of Show Award and received Double Gold honors at the 2014 International Denver Spirit Awards. The spirit also won a Double Gold award at the San Francisco Wine & Spirits Competition. "With the recent growing excitement around the whiskey category, now is the perfect time to introduce Hornitos® Black Barrel® – a one-of-a-kind product that is sure to please both tequila and whiskey fans," said Gary Ross, Senior Brand Director Tequila at Beam Inc. "We are also extremely proud of the industry's response to our newest spirit. The success of Hornitos® Black Barrel® at the 2014 International Denver Spirit Awards and San Francisco Wine & Spirits Competition serves as further evidence that this storied brand will continue its 140-year history of revolutionizing the tequila category." "The groundbreaking aging process that Hornitos® Black Barrel® undergoes is unlike anything else on the mass market and gives it remarkable depth, complexity and rich character," said Servando Calderon, Casa Sauza Distillery Director. "This tequila takes on two unique spirit profiles and truly delivers a new and innovative product with the same high quality standards that tequila aficionados have come to expect from Hornitos®." Hornitos® Black Barrel® is 80 proof and is available for a suggested retail price of $29.99 for a 750ml bottle. It can be sipped on the rocks, neat, or mixed in cocktails. For those looking to enjoy Hornitos® Black Barrel® in a cocktail, some recipes for inspiration can be found below.


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For all those who think that RINOs have a place in a conservative agenda, vis, the Republican Party, here's your proof they don't.
"One Republican voted for Pelosicare: GOP Rep. Anh (Joseph) Cao of Louisiana.
Yes, he's the one who took over corruptocrat Democrat William Jefferson's seat.
I had reservations about him on election night because of his soft-on-immigration views. But I gave him the benefit of the doubt.
If he could stand strong on limited government and fiscal conservatism, it would be worth it.
Well, since he was elected, Cao has backed the S-CHIP expansion, the $108 billion IMF bailout, and the omni-waste spending bill. And he voted to rebuke GOP Rep. Joe Wilson for calling out President Obama on his health care lies.
That is a steep price to pay for Rep. William Jefferson's removal.
Can't the GOP do better?
We can and we will. But we know where that begins. The GOP backed Cao, just like they did Scozzafava. After Specter, Snowe, Graham, and the rest of the Party Poopers, Cao should be the call to begin keep the purge going.
We need leadership at the GOP. Michael Steele isn't the leader we need. Steele needs to step down if we have any hope of returning America to where it belongs.
We gain nothing by allowing these traitors the honor of wearing a "R" next to their name, and everything by sending them back to the street.


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text: RT Book, Whole DB /z-wcorg/ DS http://worldcat.org ID 31291458 LA English T1 Ladder of years A1 Tyler, Anne., PB Knopf PP New York YR 1995 SN 0679439412 9780679439417 0679441557 9780679441557 AB A runaway wife leaves one domestic situation, only to fall into another. She is Delia Grinstead, 40, of Baltimore, the wife of a physician and mother of three. One day she decides she's had enough of being invisible, moves to another town and gets herself a job--caring for a boy whose mother has abandoned the family. By the author of Saint Maybe.


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Alumni Reception a success!
Earlier this month the Department of Dermatology held an Alumni Reception during the American Academy of Dermatology's Annual Conference in Orlando. We had a great turn out and a great […]
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[countable] an area of land in the country used for growing crops or keeping animals in, usually surrounded by a fence, etc. People were working in the fields. a ploughed field a field of wheat We camped in a field near the village.
field noun definition in On the farm topic from the Oxford Advanced Learner's Dictionary
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Hayden Panettiere leaving the Chateau Marmont in West Hollywood (1/11)
Hayden Panettiere hooked up with her old boyfriend, 6'6″ Ukrainian boxer Wladimir Klitschko, again over the holidays and now her friends are starting to throw out the "L" word . . . and I'm not talking about "long." Seriously, what's Wladimir packing down there? A foot? From Life & Style:
Hayden Panettiere's giving her relationship with Wladimir Klitschko another shot! Life & Style can reveal that the Nashville star, 23, is back together with her ex-boyfriend, 36.
"Hayden's so happy," a pal gushes to Life & Style. "They're definitely in love again and tighter than ever … They have been traveling together. He's even planning to visit her while she films in Nashville."
Damn it, how can I be mean to these two? There're in love, so who am I to judge Hayden and the Frankenstein Monster? It's not as if their relationship is any more or less weird than what George Clooney and Stacy Keibler share, or Woody Allen and Soon Yi Previn, or even Kim Kardashian and the urinals at the Source Awards. It's all about enduring love, people. What is love, you ask? Well, as far as Hayen and Wlad are concerned, love is surviving the pinksocking without needing stitches. Yes, I'm a romantic at heart.