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Identifying genes preferentially expressed in undifferentiated embryonic stem cells
The mechanism involved in the maintenance and differentiation of embryonic stem (ES) cells is incompletely understood.
To address this issue, we have developed a retroviral gene trap vector that can target genes expressed in undifferentiated ES cells. This gene trap vector harbors both GFP and Neo reporter genes. G-418 drug resistance was used to select ES clones in which the vector was integrated into transcriptionally active loci. This was then followed by GFP FACS profiling to identify ES clones with reduced GFP fluorescence and, hence, reduced transcriptional activity when ES cells differentiate. Reduced expression of the GFP reporter in six of three hundred ES clones in our pilot screening was confirmed to be down-regulated by Northern blot analysis during ES cell differentiation. These six ES clones represent four different genes. Among the six integration sites, one was at
Taken together, we demonstrate the use of a novel retroviral gene trap vector in identifying genes preferentially expressed in undifferentiated ES cells.
Stem cells offer hope of potential therapies for diseases as disparate as diabetes, Parkinson's and Alzheimer's disease. Naturally, knowledge of the intrinsic properties of stem cells must be gained before the hope of using stem cells for therapeutic purposes becomes a reality. In particular it will be important to know how these cells that retain the ability to continuously self-renew in a multipotential state can be maintained in this undifferentiated state.
There are more than a dozen different kinds of stem cells in mammals, including those that retain the ability of totipotential differentiation, the so-called embryonic stem cells or pluripotential stem cells
We are interested in identifying these determinants since they are likely to play an important role in the maintenance, proliferation, survival or differentiation of stem cells. ES cells should express the determinants typical of all stem cells. They can be easily maintained in culture and are amenable to both genetic and molecular manipulations. Thus, we have chosen to use mouse ES cells as a model system in which to isolate determinants that maintain stem cells in their undifferentiated state or promote the differentiation of stem cells into a certain lineage. One assumption for such stem cell determinants is that at least some are only expressed in undifferentiated stem cells, but not in differentiated cells. Indeed, the
Gene trap is a genetic approach that utilizes promoter-less reporters to target endogenous functional genes
The ease of using the gene trap approach and GFP FACS profiling, plus the simplicity of identifying the trapped genes, make this vector a suitable choice to target and isolate ES cell-specific genes in mice. Similar to previous studies
Construction of a retroviral gene trap vector that allows GFP FACS profiling as well as G-418 drug selection
As a first step toward the goal of isolating ES cell-specific genes, we designed a novel murine Moloney leukemia virus (MMLV)-based retroviral gene trap vector. This vector harbors a strong splice acceptor (SA) site followed by GFP and Neo reporter genes (Figure
Figure 1
Diagram of the gene trap vector eGeoN/E+pA
Diagram of the gene trap vector eGeoN/E+pA. Neo, Neo resistance gene; GFP, cDNA encoding green fluorescent protein; pA, polyA addition signal derived from the gene of bovine growth hormone (bGH); SA, splice acceptor sequence present in intron 2/exon 3 of
Isolation of ES clones in which the trapped genes are down-regulated during ES cell differentiation
The schematic procedure used to isolate candidate ES clones is illustrated in Figure
Figure 2
G-418 drug resistance and GFP fluorescence were used to isolate the trapped ES clones whose trapped genes are down-regulated during ES cell differentiation
G-418 drug resistance and GFP fluorescence were used to isolate the trapped ES clones whose trapped genes are down-regulated during ES cell differentiation. (A) Flow chart for the experimental procedure.(B) GFP FACS profiling of the ES clones. FACS plots of the ES clone 5B33 (left panel) and ES clone 5C1 (right panel) are shown with both the undifferentiated populations of cells (the green filled histograms) and differentiated populations of cells (the red unfilled histograms). Undifferentiated ES cells were grown in the presence of LIF and feeder fibroblast cells and the differentiated cells were grown without LIF and feeder fibroblast cells. "P" marks the GFP-positive population of ES cells present in the undifferentiated cells of the ES clone 5C1.
If the endogenous gene is turned off when ES cells differentiate, the expression of GFP and Neo reporters in the trapped locus will not persist and these cells will lose both green fluorescence and drug resistance. We have taken advantage of one of the conspicuous traits of ES cells; they have the tendency to spontaneously differentiate. In the presence of feeder fibroblast cells and leukemia inhibitory factor (LIF), a significant fraction of ES cells remain undifferentiated. By contrast, almost all ES cells become differentiated within four days without feeder fibroblast cells and LIF. Two populations of cells were derived for each ES clone: undifferentiated cells grown in the presence of feeder fibroblast cells and LIF or differentiated cells grown without either feeder fibroblast cells or LIF. These two populations of cells were compared for each ES clone for the level of GFP fluorescence by FACS profiling.
About three hundred ES clones were screened to identify those that displayed significant reduction in GFP fluorescence when differentiated. About 40% of the G-418-resistant ES clones we examined did not show any fluorescence in FACS analysis. We reasoned that enzyme-based drug selection is more sensitive than GFP fluorescence and thus not all G-418-resistant ES clones displayed GFP fluorescence. For the rest of the ES clones, expression of the GFP reporter appeared to remain unchanged in most ES clones when comparing undifferentiated cells and their differentiated counterparts. For example, in the ES clone 5B33 very little difference in GFP fluorescence was detected between the undifferentiated and differentiated populations of cells (Figure
Confirming the down-regulation of the candidate ES clones at the transcript level by Northern blot
The intensity of GFP fluorescence of every ES clone as well as the degree of down-regulation in GFP FACS profiles upon ES cell differentiation varied greatly among these candidate clones. To confirm that down-regulation of GFP fluorescence reflects decreased transcription of the GFP reporter gene, we resorted to Northern blot analysis to examine these candidate ES clones directly at the transcript level with a GFP cDNA fragment as the probe (Figure
Additional file 1
Supplemental figures. Northern blot as shown in Supplemental Figure S1 was used to examine the levels of GFP hybrid transcripts of some candidate ES clones that display significant reduction of GFP fluorescence in FACS profiling. Supplemental Figure S2 shows the GFP FACS profiles of both undifferentiated cells and differentiated cells derived from the candidate ES clone 5C32. Supplemental Figure S3 provides evidence suggesting that an EGFP/Neo fusion protein is toxic to the cells.
Click here for file
Figure 3
Northern blot analysis of the candidate ES clones
Northern blot analysis of the candidate ES clones. (A) The exposed X-ray film of the Northern blot with a GFP cDNA fragment as the probe. U, Undifferentiated ES cell population. D, Differentiated ES cell population. TC1, wild-type ES cells. REX-1, an ES clone with the REX1::EGFP reporter transgene. 5C32, 5C18, 5C1, 5A3 and 2G2 are gene-trapped ES clones isolated in this study. (B) The RNA-agarose gel under an ultraviolet (UV) lamp. Approximately 10 μg of total RNA was loaded for the U and D samples of the control samples and each ES clone, except that 5 μg of total RNA was applied for the ES clone 5A3 and 2G2. The positions of 18S and 28S ribosomal RNAs on the agrose gel are marked.
Table 1
Illustration of the trapped genes proven to be significantly down-regulated in the differentiated ES cells versus undifferentiated ES cells
Trapped ES clone
Matched Gene
EST Origin
A novel
Eight-cell embryo, Germ cell, ES cell
Embryo, Germ cell, ES cell
Blastocyst, ES cell
Determining the molecular identities of genes trapped in the candidate ES clones
To determine the molecular identities of the trapped genes, we used an inverse PCR approach to isolate the retroviral integration site of these candidate ES clones together with the flanking genomic sequence (data not shown). This genomic sequence was used to search the available genome databases. Indeed, we identified the integration site for 15 out of 19 candidate ES clones shown in Figure
Table 2
Illustration of ES clones that display significant down-regulation in FACS profiling but could not be confirmed by Northern blot
Trapped ES clone
Matched gene (or genomic region)
2F1 (2 integrations)
UBA52 and LYSMD1
Near CXXC6
One clone resulted from integration in the gene
The integration sites for three independent clones were localized to the same genomic locus. Indeed, the gene trap vector was inserted into the same huge intron but at different locations of a novel isoform of
Figure 5
Verifying the down-regulation of the endogenous genes during ES cell differentiation
Verifying the down-regulation of the endogenous genes during ES cell differentiation. (A) Diagrams are shown for the 5' portion of the novel embryonic isoform (upper, 2G2) as well as the most common isoform (lower, CSL) of
The gene trapped in the fifth ES clone matches two EST clones that are expressed in the blastocyst. Analysis of cDNAs for this gene suggests the presence of multiple alternatively spliced isoforms with the characteristics of noncoding RNA molecules (see below).
The identity of the gene trapped in the last ES clone, which was confirmed to be down-regulated when ES cells differentiate, remains to be determined.
Confirming the identities of the candidate genes by RT-PCR
To confirm the identities of the predicted genes trapped in the candidate ES clones, we resorted to RT-PCR analysis of the transcriptional fusion products between the trapped gene and the gene trap vector. This was performed using one primer complementary in sequence to the exon 3 region of
Figure 4
The identities of the trapped genes were verified by RT-PCR analysis of the fusion transcripts
The identities of the trapped genes were verified by RT-PCR analysis of the fusion transcripts. (A) RT-PCR products of the four candidate ES clones (2G2, 5C25, 5C11 and 5C1). The primer Xho-Junc (P1), which has a sequence complementary to the junction of the SA site and I(N) sequence in the gene trap vector eGeoN/E+pA, was used for reverse transcription in the first-strand cDNA synthesis. Another primer Bcl-2R2 (P2) complementary to exon 3 of
Sequencing of these fusion RT-PCR products not only confirmed our prediction for these trapped genes, but also provided us with the opportunity to observe the exact splicing patterns of fusion transcripts around the integration site. As shown schematically in Figure
Verification of the down-regulation of the endogenous genes
We employed either Northern blot with gene-specific probes or semi-quantitative RT-PCR to test if the expression of the endogenous genes behaves similarly to that of the GFP reporter gene in the correspondingly trapped ES clones.
We utilized a semi-quantitative RT-PCR approach to investigate the novel embryonic isoform of
Transcriptional down-regulation of the novel gene trapped in the ES clone 5C11 was verified by Northern blot using a gene-specific probe (Figure
In agreement with published findings
The novel gene trapped in the ES clone 5C11 may be a non-coding RNA gene with a very restricted expression pattern
Interestingly, transcripts derived from the novel gene trapped in the ES clone 5C11 appeared to be very heterogeneous with multiple transcription initiation sites, multiple polyA-addition signals and multiple alternative splicing patterns (Li and Leder, unpublished data). The main transcripts were apparently very large based on their positions relative to those of the ribosomal RNA transcripts (Figure
Figure 6
The novel gene trapped in 5C11 may encode noncoding RNA transcripts with a very restricted expression pattern
The novel gene trapped in 5C11 may encode noncoding RNA transcripts with a very restricted expression pattern. (A) Longer exposure (10-day) of the Northern blot as shown in Figure 5B revealed the presence of short transcripts of 5C11. These heterogeneous short transcripts, marked by a bracket, were also down-regulated during ES cell differentiation by comparing Lanes 1, 2, 5 with Lanes 3, 4, 6. (B) Short transcripts of 5C11 were predicted to form very stable secondary structures. The nucleic acid folding program (mfold) was used for the prediction [47]. One predicted folded RNA structure for a full-length cDNA is shown here as an example. (C) A diagram is shown for the exon-intron organization of one of the isoforms of 5C11 transcripts. "Int" stands for the retroviral integration site in the ES clone 5C11. This isoform is predicted to form highly stable secondary structures (Figure 6B). (D) 5C11 transcripts are not detectable in any adult mouse organs by Northern blot. Total RNA samples were derived from the following adult mouse organs: Br, Brain; H, Heart; IN, Intestine; K, Kidney; Li, Liver; Lu, Lung; O, Ovary; S, Spleen; Te, Testis; Th, Thymus; U, Uterus. Total RNA was also derived from wild-type TC1 ES cells (TC1). The gel positions of the ribosomal RNA transcripts were marked.
As has previously been shown, it is certainly possible to use a genomic approach to identify ES cell-specific genes at a much larger scale
The gene trap approach has its limitations. First, it is not designed as a large-scale approach as is DNA micro-array or SAGE and is limited by the efficiency of the vector to trap the endogenous genes. Second, although retroviral integrations are generally thought to be random, it appears that there are some hotspots for murine Moloney leukemia virus (MMLV) and other retroviruses
The infection efficiency that was achieved with our current vector was still quite low based on the number of G418-resistant colonies obtained from a 10-cm dish of ES cells infected with this vector. It is true that ES cells are notoriously difficult to infect and normally their infection efficiencies are two to three orders of magnitude lower than those of fibroblast cells
We favor our current reporter system expressing GFP and Neo as two independent proteins over a bi-functional reporter system involving the fusion of GFP and Neo reporter genes because we found that expression of the fused GFP and Neo reporter gene that we constructed is toxic to the cells even without G-418 drug selection (Supplemental Figure S3, see Additional file
We noticed that roughly one-fifth of the ES clones that had significant reduction of GFP fluorescence in FACS profiling were verifiable by Northern blot. One plausible reason for this is that FACS measures GFP fluorescence at the single cell level whereas Northern blot is based on the approximation of loading equal amount of total RNA derived from a group of cells. In this case undifferentiated ES cells proliferate very rapidly and it is likely that more ribosomal and other RNA transcripts involved in protein synthesis are produced in a given undifferentiated ES cell than are produced in a differentiated cell. Therefore, we may have under-estimated the amount of GFP reporter transcripts in the undifferentiated cells on a per cell basis by Northern blot. Another possible explanation is that the degree of reduction in GFP fluorescence varied greatly among the candidate ES clones. Indeed, almost all the clones that were verifiable by Northern blot were the ones that displayed dramatic down-regulation in FACS profiling when ES cells became differentiated.
It is also possible to carry out a similar screening in ES cells by using the well-established promoter-trap vectors containing a beta-geo marker gene encoding a β-galactosidase and Neo fusion protein, in combination with a fluorogenic β-galactosidase substrate
The novel embryonic isoform of
ES cells share many characteristics with other somatic stem cells. They all have self-renewal and multipotential capacities. Many aspects of stem cell biology using ES cells will probably be applicable to other stem cells as well. However, unlike some other stem cells, ES cells can be readily maintained in culture and are amenable to genetic and molecular manipulations. The ability to generate germline transmissible mice from ES cells also gives us the unique opportunity to look into the phenotypes
ES cells can be induced to differentiate into many cell lineages
ES cell culture
Murine TC1 ES cells were grown on mitomycin C-treated feeder fibroblast cells. The medium used for undifferentiated ES cells is DMEM (Gibco) supplemented with 15% fetal bovine serum (Sigma) and 103units/ml Leukemia Inhibitory Factor (LIF) (Chemicon). To obtain differentiated cells, the same growth medium was used and ES cells were grown without LIF and feeder fibroblast cells for four days.
Construction of the gene trap vector
Inverse PCR was used to delete the intervening sequence in the original RET vector
Retroviral infection and drug selection of ES cells
The gene trap vector was transfected into phoenix viral packaging cells
FACS analysis of ES clones
G-418-resistant colonies were individually picked, partially digested with trypsin and transferred to 24-well plates seeded with feeder fibroblast cells. After growing for 3 to 5 days, half of the ES cells derived from each clone were frozen as a stock and the rest were split into two wells of 24-well plates. One portion of the cells was grown in the medium with feeder fibroblast cells plus LIF and the other was grown without feeder cells and LIF. After being cultured for 4 days, ES cells from both undifferentiated and differentiated populations were harvested for FACS analysis.
RNA preparation and Northern blot
Trizol reagent (Invitrogen) was used to harvest total RNA from ES cells. After purification, total RNA was dissolved in DEPC-treated water. The concentration of RNA was measured with spectrophotometer and approximately equal amounts of total RNA for differentiated and undifferentiated samples were loaded for Northern blot analysis.
Total RNA was also prepared from the adult mouse organs with Trizol reagent.
Amplification of the integration sites and flanking genomic sequences by inverse PCR
Genomic DNA was prepared from each candidate ES clone and subjected to HindIII restriction enzyme digestion. After HindIII was heat-inactivated, the digested genomic DNA was treated with T4 DNA ligase. The ligation mixture was used directly as the DNA template for the inverse PCR reactions. Two rounds of the inverse PCR reactions were performed, with the PCR reaction mixture from the first round inverse PCR as the template for the second round inverse PCR by using two nested primers. The two primers used for the first round inverse PCR reaction are IR(N)-GF1 and U5-R1, with the sequences of 5'-cctgccacagacttagaatcagcc and 5'-ctcttgcagttgcatccgacttgtg, respectively. The two primers used for the nested second round inverse PCR are IR(N)-GF2 and U5-R2, with the sequences of 5'-ctctaaggaccctgattcc and 5'-gtggtctcgctgttccttg, respectively.
RT-PCR analysis of the fusion transcripts
The primer used for the first-strand cDNA synthesis is Xho-Junc with the sequence of 5'-tcgagccctgagccgta which is complementary to the sequence at the junction of SA and I(N) in the gene trap vector eGeoN/E+pA. The reverse primer used for the PCR reaction is Bcl-2R2 with the sequence of 5'-ccgtacagttccacaaag which is complementary to the sequence present in the exon 3 of
Semi-quantitative RT-PCR analysis of the different isoforms of
First-strand cDNA template was synthesized from 2 μg total RNA of undifferentiated wild-type TC1 ES cells by using Superscript II™ (Invitrogen/Gibco). One-tenth of the first-strand cDNA mixture was used in the RT-PCR reactions. Thirty cycles of amplification were performed with the Advantage GC cDNA polymerase (BD Biosciences Clontech). The primers used are P1 5'-ccttcaagatatccagcaag, P2 5'- cctataggccaacatttgag, P3 5'- caaacgactcactagggaag, P4 5'- cacagggttgagactcttg and P5 5'- gtaatgccctccggttttcctc.
Authors' contributions
XL conceived of the study and PL participated in its design. XL carried out all the experimental studies. Both XL and PL read and approved the final manuscript.
We want to thank Yasumasa Ishida and Nissim Benvenisty for their advice when this project was initiated. Racheli Eiges and Nissim Benvenisty kindly provided the REX-1::EGFP reporter construct. We are grateful to Montserrat Michelman for her help with ES cells and to Juanita Campos for her help with FACS analysis. We thank Alex Bishop, Nicholas Chester, Holger Babbe and Markus Dettenhofer for the comments on the manuscript. X. Li was a postdoctoral fellow of the Helen Hay Whitney Foundation.
Stem cells – hype and hope
Dedifferentiation of mammalian myotubes induced by msx1
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Changing potency by spontaneous fusion
A family of octamer-specific proteins present during mouse embryogenesis: evidence for germline-specific expression of an Oct factor
Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4
A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo
A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells
Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells
Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice
A gene trap approach in mouse embryonic stem cells: the lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice
Gene-trap mutagenesis: past, present and beyond
From ES cells to mice: the gene trap approach
An induction gene trap screen in embryonic stem cells: Identification of genes that respond to retinoic acid in vitro
An induction gene trap for identifying a homeoprotein-regulated locus
A gene trap vector system for identifying transcriptionally responsive genes
Expression trapping: identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells
Efficient gene trap screening for novel developmental genes using IRES beta geo vector and in vitro preselection
Differentiation trapping screen in live culture for genes expressed in cardiovascular lineages
RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells
High-efficiency gene transfer and selection of human hematopoietic progenitor cells with a hybrid EBV/retroviral vector expressing the green fluorescence protein
Rex-1, a gene encoding a transcription factor expressed in the early embryo, is regulated via Oct-3/4 and Oct-6 binding to an octamer site and a novel protein, Rox-1, binding to an adjacent site
Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells
A novel nuclear protein with zinc fingers down-regulated during early mammalian cell differentiation
Comprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neurons
Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity
Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells
Identification and characterization of ZFP-57, a novel zinc finger transcription factor in the mammalian peripheral nervous system
Keeping a good pathway down: transcriptional repression of Notch pathway target genes by CSL proteins
"Stemness": transcriptional profiling of embryonic and adult stem cells
A stem cell molecular signature
Gene trapping methods for the identification and functional analysis of cell surface proteins in mice
Targeting survival: integration site selection by retroviruses and LTR-retrotransposons
Promoter interactions in retrovirus vectors introduced into fibroblasts and embryonic stem cells
Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Fluorescence-activated sorting of totipotent embryonic stem cells expressing developmentally regulated lacZ fusion genes
Genomic organization of mouse J kappa recombination signal binding protein (RBP-J kappa) gene
Loss of immunostaining of the RBP-J kappa transcription factor upon F9 cell differentiation induced by retinoic acid
Notch pathway molecules are essential for the maintenance, but not the generation, of mammalian neural stem cells
Primitive neural stem cells from the mammalian epiblast differentiate to definitive neural stem cells under the control of Notch signaling
Lineage choice and differentiation in mouse embryos and embryonic stem cells
Translation of NRF mRNA is mediated by highly efficient internal ribosome entry
Quikfold server


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First Meeting of the Ministers and High Officials in charge of Information and Media of the Turkic Council has kicked off in Astana.
SIA reports that Azerbaijani President's Aide Ali Hasanov, Minister of Culture, Information and Tourism of the Kyrgyz Republic H.E. Altynbek Maksutov, Minister of Investment and Development of the Republic of Kazakhstan, Asset Issekeshev, Director General of the Turkish Radio and Television Corporation Şenol Göka attended the event. The Meeting was moderated by Ambassador Ramil Hasanov, the Secretary General of the Turkic Council.
The Ministers and High Officials stressed the importance of developing cooperation in media and information sphere among the Member States of the Turkic Council, and its crucial role in promoting the communication and mutual understanding and raising the political, cultural, economic and social awareness about the Member States.
They also expressed their support for cooperation among national news agencies, periodicals, television and radio networks both public and private, as well as the unions of journalists through holding forums, seminars, workshops, training programs and exploring new opportunities for further cooperation.

subset: commoncrawl


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A couple of years ago for Christmas, my sister, Whitney, took all my grandmother and grandfather's love letters and made them into a book.
It was called Letters from Box 54.
There, in print, were all the everyday, the simple, the overlooked and the ordinary of our grandparent's lives.
But in those moments–shared from letters written long ago with paper and ink, you could catch a glimpse of the overwhelming, incredible, amazing love they shared for each other….
…and see the extraordinary written in every line.
We all loved the book so much that we begged her for more history. "Make a family tree," we'd say. "History would come alive under your watch," we'd encourage. "You are the perfect family-treeer. No one could make a family tree like you," we'd implore.
But to no avail.
She would smile and laugh roll her eyes and shake her head and explain that family trees were a challenge she didn't really want to take on.
Until this year.Continue reading


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The ostensible purpose of a library is to preserve the printed word. But for fifty years our country?s libraries?including the Library of Congress?have been doing just the opposite, destroying hundreds of thousands of historic newspapers and replacing them with microfilm copies that are difficult to read, lack all the color and quality of the original... more...
The Knights HospitallerBoydell & Brewer 2001; US$ 34.95
A vivid, concise history of the Order of St John of Jerusalem, founded to care for sick and poor pilgrims, defenders of Christendom for almost seven hundred years. more...
Incomparable Captain CadellAllen & Unwin 2004; US$ 27.22
In an age when exploration was at its peak, Francis Cadell?s career reads like the quintessential boy?s own adventure. Born and raised on the Leith waterfront into a large family of ambitious achievers, he was lured early to a life on the high seas. In 1836 at the age of fourteen, he sailed to China where he ran supplies and ammunition to the besieged... more...
Though you might not encounter the subject in Artforum or stumble across it at Sotheby's, the thriving business of erotica is a mixture of sophistication and seduction, an underground world of eccentric artists and serious collectors. In Sex Collectors, Geoff Nicholson hunts down an assortment of these obsessives around the world. From the... more...


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History Repeats Itself
this wiki
Back when I was young, around ten years old, I had an obsession with the paranormal. The act of communicating with someone who had passed was just so fascinating to me. I loved it... Everywhere I went, I would search for ghosts, even an innocent trip to my friend's house turned into me searching a locked room for entities. When I was ten, I experienced a house fire. It wasn't terrible, because most of it was smoke damage. We moved to an apartment for two months while the workers cleaned and repainted things. Ninety percent of our things were tossed. The workers often complained of hearing crying, slamming, and footsteps coming from our house. It's expected: it's an older house. Now, the fire that occurred was nobody's fault because it was caused by a heater malfunction, I don't want anybody thinking that it was arson.
The fire really woke me up to things, as I had previous experience with a fire but seven months before. I refuse to talk about that, but I'm going to say that I had post traumatic stress disorder because of it. The fire that happened at my own house left me with insomnia, paranoia, and several problems revolving around people entering our house. Now, just recently, my mother had told me that about 50 years ago, the top floor of my house was an apartment that experienced a tragic fire. It is not known whether any lives were lost in the fire, but it really makes me wonder whether there will be another fire at my home in about 40 years.
This is just a personal theory, and my first story I ever wrote in this genre. Thank you...


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Postings by My Family
Siberian Husky > HELP!! I\'m ready to call the breeder and see about a return for my 3-month old Siberian Husky!!!
Siberian Husky > Light Red Huskies
Siberian Husky > ✔ A head-count of all the Dogster Huskies ✔
PLEASE NOTE: Due to the rapid nature of forum postings, it's quite possible our calculation of the number of ensuing forum posts may be off by one or two or more at any given moment.


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Geoff Thorpe <ge...@geoffthorpe.net>
|Re: [PATCH] openssl configuration
|Fri, 28 Feb 2003 15:45:45 GMT
Hi Thom, * Thom May (email@example.com) wrote: > * Geoff Thorpe (firstname.lastname@example.com) wrote : > > It would perhaps make sense to provide a "--force-ssl-ver" type of > > option that would bypass version checks, and then have any version > > checking failure text point out the existence of "--force-ssl-ver". This > > way, the more determined users can force configure to bypass that, > > whilst it still provides a certain safety-net for the more naive and > > less intrepid against accidently meddling with known-to-be-out-of-date > > support libraries. > > (un)?fortunately most vendors prefer to backport security fixes rather than > release new versions of software into stable releases since backports are > far less likely to interfere with already tested and correctly integrated > software. Thus the average user is unlikely to *know* that they would need > to force an ssl version. Less intrepid users are far more likely to be > following vendor security updates ;-) Well, this is an issue for the httpd developers to decide on, not me. I put the version checks in because (a) to me (again, outside the httpd sphere of view) it seemed logical, but more importantly (b) the existing autoconf checks did essentially the same version checking but in a more fragile form and for a now out-of-date threshold. I'm just as happy to axe the version check or set up warnings in its place. My goal here is to fix the openssl checks so that the currently-incorrect path, include, and linker handling is corrected. Eg. if I set up my system with non-standard PATH, INCLUDES, ld.so.conf, etc - anything following normal autoconf practice will be fine but apache's ssl/tls handling will not. Likewise if --with-ssl=<dir> is used with a relative path it will succeed the configure checks but fail compilation. W.r.t the version checks, I don't feel passionately about it one way or the other - by all means tell me what the consensus is and I'll rejig the patch for that. > I don't think we should have enforced version checks for this; if we do > detect an old version I think the most we should do is to suggest that the > user checks with their vendor that they have the most uptodate release for > their OS; and that said release fixes the (known) security holes. If that's what people want, that's what I'll do. Should I simply leave in a version check equivalent to the existing one (0.9.6e) and not rock the boat? Or should I turn the version error into a version warning? Cheers, Geoff -- Geoff Thorpe firstname.lastname@example.com http://www.geoffthorpe.net/
|View raw message


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Hard Rock Stadium has partnered with the SunPass program to offer fans a convenient, less expensive parking payment option for attending Miami Dolphins games and all other stadium events. As you approach the stadium, look for the SunPass Plus logo at the parking gates.
To check to see if you are enrolled in SunPass Plus, log into your account at SunPass.com and click on "TRANSPONDERS" at the top of the page to confirm your SunPass Plus status. You can also check your SunPass Plus status using the SunPass app, available for iOS and Android devices.
SunPass Plus customers will have the convenience of using the program and paying less than the cash customer entering Hard Rock Stadium for all stadium events.
Uber is the preferred ride sharing partner of the Miami Dolphins, with Hard Rock Stadium hosting a designated Uber Zone, providing one convenient location for drop and pick up for fans to get easily in and out of games while significantly alleviating traffic.
The Uber Zone, located in Lot 14 at Hard Rock Stadium, will make getting to and from any event easier than ever before. Request your ride to Hard Rock Stadium and when you arrive, your Uber driver will drop you off at the Uber Zone, so you won't have to wait to find a parking spot. Then when you're ready to request a ride, simply head to the Uber Zone. Once you're matched with a driver-partner, you'll receive a text message letting you know what parking spot in the Uber Zone you'll be leaving from.
Additionally, the Dolphins are the first sports team to host an UberTailgate, providing fans with the opportunity to tailgate and use Uber to enter the game with an end-to-end tailgating experience at their disposal.
Transit Tips:
Did you know you can take Miami-Dade Transit to Hard Rock Stadium?
For all 2016-17 Miami Dolphins home games, Miami-Dade Transit will offer a special Sunday service from the Earlington Heights Metrorail Station to Hard Rock Stadium.
Simply take the Metrorail to the Earlington Heights station and transfer to the Metrobus route 297.
You can also catch the Metrobus route 297 at the following Metrorail stations: Brownsville and Dr. Martin Luther King Jr. (Miami-Dade North campus is no longer a stop)
Service will commence two hours before kickoff and run until two hours after the game.
Click here for a full route map, and download the Miami-Dade Transit Tracker app to start planning – and tracking – your next trip!
Visit miamidade.gov/transit for more information.


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It's common that reality TV stars often evolve their on-screen career into other entertainment areas, and soon to be in the works appears to be a Kim Kardashian music career.
Although this is all just speculation, it has been reported that she was recently working with songwriter and producer Terius 'The Dream' Nash. As Kardashian manages to remain in the spotlight on numerous occasions, these Kim Kardashian initiatives will provide you with a recap on all her headlines.
31 Kim Kardashian Initiatives
More Stats +/-
Professional Photoshop Fails
Unretouched Nudetorials
Photoshop Fails
Reality TV as Exercise
Bare-All Celebtography
A Kim Kardashian Music Career May be in the Works
Nov 3, 2010By: Summer Luu -


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Home / DVDs / Opera / Rigoletto (DVD) - Damrau, Flórez (Item# 1000005857 )
Rigoletto (DVD) - Damrau, Flórez
Item# 1000005857
Rigoletto (DVD) - Damrau, Flórez
Composer: Giuseppe Verdi
Release Date: October 25, 2010
Conductor: Fabio Luisi
Diana Damrau
Juan Diego Flórez
Željko Lucic
Orchestra: Sächsische Staatskapelle
Label: EMI Distribution
Language: Italian (Stereo)
Subtitles: German
Run Time: 90 minutes
Region: Region Free, Worldwide
One of the most popular operas in the repertoire, Verdi's riveting drama was first seen in 1851 at Venice's Teatro La Fenice. Together with his next two works, Il Trovatore and La Traviata, it formed the basis for the composer's lasting success. For his new production, director Michael Mayer has moved the action from 16th-century Mantua to 1960s Las Vegas: "I tried to imagine a recent world that captures the decadence of the Duke's palace, where the participants are in pursuit of power, money, and beauty," he says. Las Vegas in the '60s is such a world, where a kind of prankster energy could go bad."
Average of 2 customer reviews:
May 2013
Jilly Cooke
Iowa City, IA
April 2014
Gender: female
How Long Have You Been Using This Product: Less than 1 month
You might also like......


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You missed it This great deal for The I Spa is over. But don't despair, we've got more great deals every day.
Related Deals That You May Like
The I Spa
Spa Pedicure and Massage or Facial in an Upscale Environment
89% recommendedin LivingSocial customer ratings
Situated in the elegant InterContinental Hotel is The I Spa, one of San Francisco's most luxurious getaways for pampering. Treatments are personalized, relaxing, and perfect for improving your appearance and state of mind:
• $110 ($185 value) for an Essential pedicure & 50-minute Swedish massage or custom facial
• $70 ($120 value) for a 50-minute Swedish massage
• $140 ($240 value) for a 50-minute Swedish massage & 50-minute custom facial
Luxury All Around
Whatever package you opt for, with The I Spa, you're in for a treat. The Swedish massage uses flowing, moderate pressure to yield a deep feeling of relaxation, improve circulation, ease aches, and release tension. The custom facial is a European-style treatment designed to provide great results while focusing on relaxation. It includes deep cleansing, gentle exfoliation, extractions, skin-specific mask, and a sedating massage, finishing with moisturizing. The Essential pedicure is the spa's ultimate in nail care experiences. It begins with a foot soak, revitalizing cuticle treatment, and gentle foot exfoliation. Next, your feet will be bathed in warm, therapeutic paraffin and followed by a revitalizing foot massage. Finally, toenails will be painted with the polish of your choice, or, if you prefer, left bare.
The I Spa's Website
PAID VALUE EXPIRES ON May 26, 2022
PROMOTIONAL VALUE EXPIRES ON December 2, 2013


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ED Magedson – Founder
Ripoff Report
AGC Industries, Inc1555 E Flamingo Suite 421 las vegas, Nevada United States of America
Does your business have a bad reputation?
Fix it the right way.
Corporate Advocacy Program™
Parisi Communications promised me that I would get money from grants! They failed to give me a grant! I never got one! This is a scam.
I paid a lot of money for them to write one but it never was done - that is I never got a grant!
CONTACT US America's Grant Center, Division of AGC Industries, Inc. 1555 East Flamingo Road, Suite 421 Las Vegas, NV 89119 Contact Information: Toll Free (888) 543-8881 Fax: (702) 473-9502 Email: firstname.lastname@example.com
This report was posted on Ripoff Report on 10/04/2009 11:22 PM and is a permanent record located here: http://www.ripoffreport.com/reports/agc-industries-inc/las-vegas-nevada-89119/agc-industries-inc-parisi-communications-inc-american-grant-center-promised-me-money-in-g-504327. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
Ripoff Report has an exclusive license to this report. It may not be copied without the written permission of Ripoff Report. READ: Foreign websites steal our content
Click Here to read other Ripoff Reports on AGC Industries, Inc
If you would like to see more Rip-off Reports on this company/individual, search here:Search Tips
In order to assure the best results in your search:
#1 Author of original report
SUBMITTED: Friday, October 16, 2009POSTED: Friday, October 16, 2009
If you are a upstanding citizen - then why are you renting space from a illegal business?
A business that has no telemarketing license no bond for any state you call into - you have AGC industries has no license to operate a telemarketing firm in Nevada!
Parisi was fined and is under investigation by the Clark county division of license and bonding - as well as the DA for fraudualent business activities!
You have no bond to operate a license in NV or Clark County. You are not registered nor is Parisi. The records speak for themselves! The tickets and DA investigation are public information.
And yes - always blame someone else for your short comings. These posts are real - as real as your scam. If you were legit then why are you scamming people out of money for grants. When they look for you on the web - you have no license! Do not worry. The DO NOT CALL people are on to parisi too! You rent and conduct business as a illegal telemarketing firm which is illegal no matter who you blame.
You are no navy seal. I have never known a navy seal to operate a shim sham business without doing a full back ground on license - bonding and proper DNC registration.
Are you an owner, employee or ex-employee with either negative or positive information about the company or individual, or can you provide "insider information" on this company?
#2 General Comment
SUBMITTED: Monday, October 05, 2009POSTED: Monday, October 05, 2009
Are you an owner, employee or ex-employee with either negative or positive information about the company or individual, or can you provide "insider information" on this company?
#3 REBUTTAL Owner of company
SUBMITTED: Friday, October 09, 2009POSTED: Friday, October 09, 2009
My name is William W. Barr, Chairman & Chief Executive Officer of AGC Industries,Inc. We have a division called America's Grant Center. We Started up our operations in the space we are renting from Parisi Communications on September 2, 2009. We have a staff of Grant Writers who work directly for me. To date, we have not accepted a penny from any person or company. We demand proof of these allegations which we suspect are coming from a man involved in a dispute with Danial Parisi. The version I get is that Troy Allen Melquist, a scam artist who is currently being pursued by the authorities is putting up these phony accusations. To see what kind of a scumbag we are dealing with go to(((Redacted))) I am an upstanding member of my community, a home owner, a retired Navy Seal with a 100% disability. I might add that I am one of the oldest living Eagle Scouts in America. I have never scammed anyone in my life and neither has my corporation or anyone who is an Officer or who sits on my board of Directors. Heck, I haven't even had a parking ticket for over 40 years. As a disabled veteran, I resent gravely these false accusations and demand that they be removed from your website at once. You are welcome to check with the State of Nevada or the BBB, and you will see that we run a completely forthright and honest operation. Further, we have never had one complaint filed against us. Should we ever have a complaint from a Client, we would immediately take steps to correct it. However, as of yet, that hasn't happened. You are welcome to call me at 1-888-543-8881, ext 102, my direct line in the Research Department, and I will be happy to answer any and all questions you may have. I am proud of my people and my company and its division, America's Grant Center. We don't hire anyone who has less than an impeccable job track record. My Grant Writers all have college degrees with some even having masters degrees and law degrees. They are all experienced in Grant Writing and funding. Our mission statement is "The Rebirth of American Business." Thank you for having a rebuttal page and letting me tell my side of the story. Have a great weekend.
Are you an owner, employee or ex-employee with either negative or positive information about the company or individual, or can you provide "insider information" on this company?
Advertisers above have met our
strict standards for business conduct.


text:
We have a user who always receives two emails from the helpdesk for any ticket she creates or any updates to any of her tickets. I have not been able to find the same scenario so far in the community.
We are using PO3 through a lotus notes server. I have searched the People section and there is only one entry for this user. She is the only person this happens to.
Anybody have any ideas?
Many thanks,
4 Replies
Jun 22, 2012 at 10:16 UTC
Are both emails going to the same address or is one of them a distribution group?
Jun 22, 2012 at 10:22 UTC
Both going to the same address.
Jun 26, 2012 at 11:01 UTC
Hi Folks,
As strangely as it started it has stopped! Could not find any reason for happening and the same for it stopping!!
Jun 26, 2012 at 11:26 UTC
Glad to hear it started working as expected. Like you, I'd still like to know why it was doing that but...
Lets face it, if this tech stuff always worked as advertised we would all be out of jobs (or yobs as it's pronounced here in California).


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Home Cinema
To create a memorable movie experience at home, a home cinema system with large, clear picture and dynamic surround sound effects is essential. The two main television types are LCD or plasma flat panel TV's with screen sizes ranging from 32" to 55" or more. A TV such as this can double as the family Television for the watching of news, and general TV programmes, but also act a a great home cinema screen. We specialise in supplying Loewe TV as it is far better engineered than the mainstream brands of TV and has infinitely better aesthetics and picture quality. Internet streaming of content is now commonplace in TV's, with You Tube, BBC iPlayer and other content freely available. Alternatively, for those with the space, a front projector and matching screen bring big images to your home cinema, at really affordable prices.
The centre piece of a home cinema system is the AV receiver, which is the connection hub for all of your sources (Blu-ray player, DVD player, Sky, Apple TV, Playstation 4 etc) , your TV/ projector and also powers your 5.1 surround sound speaker package. Make sure the AV receiver you choose has enough HDMI inputs for your needs, and that if you want to stream music to your home cinema system from your home network, make sure it has an ethernet port. Power outputs of AV receivers varies widely too, so choose one that will comfortably drive your home cinema speaker package. Good quality speaker cables and banana plugs complete the system.
HDMI cables are the replacement for the SCART cable, enabling digital audio/ video and control signals to be sent to and from each component in your home cinema system. You may also need digital cables ( coaxial digtial or optical digital ) to route the digital sound output of your SKY box to the AV receiver. Also, don't forget that your subwoofer will need a long audio interconnecting cable to hook up to your Home Cinema Receiver or HDMI amplifier.
Latest reviews for Home Cinema
Average Rating :
Total Reviews:2
Marantz UD7007 Blu Ray Player
Superb Music Quality And Movies
I wanted a player that could play not just bluray, but all of my music collection too, some of which is in SACD and DVD Audio - the UD7007 has impressed me greatly with it's excellent audio quality and video too.
Onkyo TX-NR3010 AV Receiver
Bags Of Power And Facilities
Onkyo have had a habit of producing a lot of award winning amplifiers in the past few years and have a range to rival any of their competitors. In the high end series you have 3 potential options I chose the middle model to trail out for a few weeks and to see what you get for £2000. Design & Construction Now for the boring part, as you would expect the TX-NR3010 comes with a host of connections and no less than 9 HDMI inputs and two outputs, so you shouldn't have problems wiring in your multiple high definition devices. The speaker options are many with the main system being a 9.2 channel amp (& for all you people who don't think that's enough you have an 11.4 analogue output to fulfil your entire home cinema needs) & 160 watts per-channel will be more than ample to power your speakers. As with most AV receiver's you have a range of networking options to make it the hub of your entertainment such as internet radio, last.fm & Spotify & with the ability to play all of your flac & apple loss less files quality music is just a fingertip away. Performance When it came to giving this a good I try out I decided to attack it on three fronts, 1-Bluray 2-Gaming & 3 music streaming. BLU-RAY On the blu-ray front I went with star trek and from the opening volley of shots I was blown away with the power, the base was hard but but with no loss detail dealing exceptionally well with a difficult start. Where the amp really came into it's on was the first warp jump the dynamics were mind blowing, I was expecting to lose a little of the detail but it was composed & controlled. GAMING On the gaming front I went with my favourite game Mass effect 3, this has the feel of summer blockbuster with massive movie sequences & intense game play , from the sharpness of the dialog in the attack on earth to the massive base drops of the reaper attack the onkyo dealt with the dynamics beautifully. In solid game play the separation of gun fire & vocals was tight & clear with a layer of depth I hadn't heard on my previous amp. MUSIC/2 CHANNEL When it came to music I listened to Linkin Parks 'a thousand suns' this was a little more experimental and cutting edge and used numerous genres but I find it really does test most systems. In the track 'waiting for the end' the amp makes the most of the rip soaring ballad, the verses are subtle with great depth & explode with chester's massive chorus. When it comes to the harsher 'wretches & kings' again the amp deals with harshness & power with confidence. Conclusion As I said in the beginning there are a lot of high end amps on the market, and at this price point you would expect a lot for your money and I'm happy to say at the Onkyo delivers on almost everything. The amp has no problem with power, but it's how it controls that power that really impresses, no matter the input. Detail is not the sharpest out there but with the right speakers and set up its no issue.


text: Dansville man crashes into parked cars GENESEO: A Dansville man is facing multiple charges after a motor crash on West Lake Road. Shawn Schledorn, 40, struck several parked cars with his jeep. On May 19, the Livingston County Emergency Communications Center (E-911) received a call reporting a motor vehicle crash on West Lake Road in the Town of Geneseo. The caller reported that a vehicle had struck several parked cars. Deputy Shawn Whitford arrived on scene and observed that a Jeep had struck two parked cars. Schledorn was interviewed and a DWAI investigation ensued. May 25 at 12:31 PM


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Originally posted on SteelerAddicts | Last updated 7/3/12
Something interesting, at least I think so, when it comes to the NFL salary cap status of all NFL teams as they head into training camp in a few weeks now; how close just is everyone to the cap? When you look at the Steelers, Bengals, Browns, and Ravens it's easy to tell just why two teams have been doing it right and why the other two teams have been doing it wrong. Now before we get into the figures, yes the Bengals and Browns have both had good drafts the last few years and there is cause for optimism in their continued improvement. However the previous bad drafts, poor free agency moves, and just general head scratching managerial decisions of the past reassure you that the true top front offices run their teams in a manner that is condusive to annual success. The following excerpt from an article on NFL.com shows the available cap space for all teams currently. For the AFC North you can see that the Cleveland Browns and Cincinnati Bengals are in the top 5 in terms of available...
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text:
Customized Packaging is an opportunity to share your brand value.
Start with DCGpac - We help !
Please click here for more details
|Brown Reflector Box
|Size or Color
|Image Gallery
Shipping goods means a lot of responsibility. We need to make sure that all the goods reach our customers or the intended persons in a safe and secure condition. Since the recent surge in e-commerce in the country, more number of goods is delivered each day than ever. When we have to make so many deliveries, we also have to take care that they are safe while in transit. These brown reflector boxes are the perfect packaging boxes when you have to send goods in long distance shipping. You can get the brown reflector box online from DCGPAC and be satisfied of all the deliveries you make.
If you need a box for shipping and packaging, this brown reflector box is the perfect option for you. It is sturdy, strong and can take a lot of compression from external factors while in transit. We all know how rough it can get while handling the packaging boxes during the deliveries. To make sure that their condition is uncompromised with, we need to use these brown reflector boxes.
These brown reflector boxes are largely used for electronic items and as some of them are fragile, they ensure the good's safety to the core. These are 5 ply boxes which mean that they are highly robust in nature and will help a lot in protecting your goods. Mostly, e-commerce companies can use these brown reflector boxes for all their shipping needs.
The quality of the brown reflector box is very high as they are made with 5 ply and also are of 180 vk and 120 sk which makes them stronger than the other boxes available in the market. They can be used for a wide range of purposes. The best part is that these boxes are 100% recyclable and eco-friendly too.
DCGPAC offers the best solutions for all your packaging boxes needs. You can buy brown reflector box online at extremely low and affordable prices. With DCGPAC, you also get the benefit of quick deliveries right at your doorsteps.


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Author has written 10 stories for Harry Potter, Naruto, and Hunger Games.
Name: Wordy.
Age: Nineteen.
Currently reading... The End of Food.
Occupation: College student.
I, TheWordFountain, do solemnly swear to review all the fics I read, regardless of the number of reviews, its age or anything else.
I have joined the revolution.
I, TheWordFountain, have challenged myself! I'm proud to be a part of the HPFC forum.
Please make note that all stories written in '08 and '09 are from before my hiatus! They are not good reflections of my current writing (except maybe "A Field of Buttercups," which I love)!


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WAUBAY — Dick and Sylvia Dargatz will celebrate their 50th wedding anniversary with an open house Saturday, Sept. 14 from 1 to 4 p.m. at Bitter Lake Lodge, 14307 446th Ave., Waubay (one mile south of Waubay). All friends and relatives are invited to attend. No gifts, please.
Cards and greetings may be sent to them at 44565 133rd St., Waubay, S.D., 57273.


text:
Herold, 32, makes four trips a year to his hometown of Boston, at times paying as much as $400 for plane tickets.
He found he could save money by comparison-shopping with SideStep, paying just $216 for a recent round trip he booked with the travel search engine.
"They haven't let me down yet," he said.
Not all online buyers have found such bargains, but they are turning increasingly to comparison-shopping engines when they're considering a purchase of everything from baby items to tires.
Last month, 46.6 million Internet users — or almost one-third of the total U.S. Web audience — visited a comparison-shopping site, according to Nielsen//NetRatings .
To put that number in perspective, online dating sites received about half as many visitors in April, the audience measurement company said.
New innovations are making it faster for online comparison-shopping services to gather data, and more retailers are participating in them as they become more popular.
As a result, comparison sites that once catered only to techies in search of rock-bottom prices on laptop computers, digital cameras or other gadgets have grown broader and more sophisticated.
As of April, Shopping.com was the market leader followed by BizRate.com and NexTag.com. All offer a broad range of mainstream products like blenders and baby car seats as well as electronics.
Not to be left out, the big Internet services are also in the mix.
Yahoo, which runs the most-visited online destination, recently bought European comparison-shopping engine Kelkoo amid efforts to beef up its Web search and shopping services.
On other fronts, Silicon Valley-based Yahoo in March relaunched its Autos channel, where users can compare up to four cars on price, features and performance — something many stand-alone engines cannot provide.
And Google recently added a link to its Froogle comparison site on its main search page. According to Nielsen//NetRatings, that service got more visits in April than older rivals PriceGrabber.com and mySimon, even though it is still in test mode.
Among specialists, SideStep's downloadable tool allows travelers to search dozens of sites for real-time prices on hotel rooms, rental cars and flights — including those on low-cost carriers like JetBlue and Southwest Airlines .
Because it pops up when users are searching for travel-related items, SideStep also can be used to do side-by-side price comparisons.
Apples to oranges
Shopping.com has filed for an initial public offering and other segment players are mulling a similar move as they bask in their popularity and accompanying hype.
Part of their success has come as large retailers realized that comparison sites can drive significant traffic to their own online stores while sparing consumers the hassle of having to jump from site to site in search of a deal.
Even so, the industry's biggest players are still missing out on some deals. For example, Shopping.com recently showed a Graco ComfortSport car seat from discount retailer Target for $79.99, but did not have a price from Wal-Mart, whose site was selling a model for $78.74. Still, the lowest price was $67.99 from Baby Universe.
That example also underscores a common consumer complaint about the difficulty of making apples-to-apples comparisons because of such factors as differing model numbers or price quotes that exclude shipping and other costs.
As with many other commercial Web services that track information about user habits, comparison-shopping sites' privacy policies have sometimes ruffled feathers.
While Shopping.com has received praise from consumer privacy advocates because it gets users' permission before sharing information, some consider SideStep's technology spyware because it tracks what users do on the Web.
"We hold any information you provide with the utmost care and security, and will not use it in ways to which you have not consented," SideStep said on its Web site.
Meanwhile, some consumers find they need to look beyond the price-comparison sites before they make their purchase decisions.
Jennifer Blake, a former marketing executive at a Northern California-based retailer, tried out some sites in her hunt for a new car seat for her 10-week-old son, who is about to outgrow his infant model.
Blake, a self-confessed Consumer Reports junkie, said that in this case she found Shopping.com's Epinions user reviews less helpful than safety test data from a respected source.
"What one mom says about how easy it is to use isn't as important as the safety," Blake said.


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Why palliative and hospice care matters
Significant gains have been made in the fight against HIV/AIDS and other chronic disease through palliative and hospice care – and we should mark World Hospice and Palliative Care Day next week by learning more about, and raising funds for, those who support people with life-threatening illnesses and their families.Read more
A day in the life of a home-based care worker
Commitment to helping those in need often goes unnoticed – but the dedication of home-based care (HBC) workers such as Francinah Ndileka Mjanyelwa quietly transforms lives, and whole communities.Read more
Global Social Service Workforce Alliance helps those who help others
Those in Tshikululu's health sector are familiar with a USAID initiative called CapacityPlus, a global alliance of healthcare workers and those assisting them in order to realise the UN's Millennium Development Goals. Now an equivalent global alliance for the social service sector has been formed, called the Global Social Service Workforce Alliance.Read more
The shift toward impact in Corporate Social Investment
Historically, corporate social investment has been mostly philanthropic. Over the past five decades we have seen enormous shifts in the practice of social investment – it has grown in complexity and in the face of demands for greater accountability.Read more
Moving charity beyond Mandela Day
Mandela Day saw corporates and employees extending goodwill across South Africa through various charitable giving. Classrooms were painted, blankets were collected and sandwiches were made for the needy. All this was done in the spirit of inspiring individuals to take action and help change the world for the better. And as wonderful as this type of charitable day is – bringing out the compassionate side in most people – the question posed now is how we extend the attitudes and activities beyond just one day.Read more
Tshikululu/GIBS Dialogue Programme partnership
Tshikululu sits at the confluence of corporate South Africa, civil society, communities, and government policies. As such, we are committed to expanding and facilitating conversations around South Africa's growth and potential.Read more
Tshikululu turns fifteen
Fifteen years of excellence, innovation, integrity and care. Fifteen years as leading social investment advisors and managers.Read more
The case against charity
'No man is an island, entire of itself; every man is a piece of the continent, a part of the main.'
John Donne, Meditation XVII
Grantmaking, and corporate social responsibility more generally, is the conduit between the capital of corporate South Africa and the NGOs that have taken it upon themselves to shore up the work of the state and assist those who have the least. Why is this money given? Sometimes out of a sense of ethical responsibility, sometimes out of a need to conform to governance imperatives and legislation, sometimes because it accrues public relations benefits, sometimes out of a sense of charity. Not all of these motivations are noble. One; charity, is pernicious.Read more
The case for giving
This paper was supposed to be about reporting. I was supposed to tell you about what constitutes an effective report, and how both donors and their NGO partners can use good reports to help maximise the impact of their work.Read more
Counting the benefit; making sure the benefits count
Since 2009, the development sector has made a notable shift in the monitoring and evaluation (M&E) practices of corporate social investment (CSI) programmes.
Trialogue reported in their 2012 CSI Handbook that the monitoring of programmes has improved, from the simple tracking of expenditure to tracking outputs and outcomes indicators and conducting site visits to funded projects.
While the attention on more and more accountability for the results of social investments increases, we are yet to see significant strides in the evaluation component of M&E.Read more


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|Message view
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|« Date » · « Thread »
|Vadim Gritsenko <va...@reverycodes.com>
|Re: [RT] Official access to the current sitemap manager
|Mon, 23 Feb 2004 13:18:25 GMT
Carsten Ziegeler wrote: >Currently (2.1), we have a static method at the CocoonComponentManager >that allows to get the current sitemap component manager. >This is useful in some scenarios when you want to access a >sitemap component. If your own component is declared not in the >current sitemap but in any parent than usually it can't access >the components declared in the current sitemap. >E.g. the SourceWritingTransformer needs access to the serializers >for writing the content. (Let's not discuss if the SWTransformer >is the best solution for the task it does - it's just one >example). > > But SWT should get serializer from its own sitemap - don't you think? Otherwise, if it will use current sitemap, and current sitemap overrides some of the serializers, results will not be what is expected. And if in nested sitemap you use SWT and want it to access locally declared serializers - then you should declare SWT in the same sitemap too. WDYT? Vadim
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Website: http://www.jaydempseyband.com/
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The fairgrounds in a great place to hold your next event! With 22 acres of fence enclosed area and 12,000 square ft of open builiding spaces; we can house anything from a car show to a company picnic.
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Pop2adz.com is a 1 year old domain, situated in United States. The domain is linked to the IP address 184.108.40.206 which is provided by the hosting company Database by Design, LLC.
It was registered by Manik Mia , who can be reached at . The site is owned by pop2adz.com. Registration details show that it was registered on 10 Jan 2016 through 1 and 1 and will expire on 10 Jan 2017.
The site is being served through Apache.


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Jeff Blatnick on Randy Couture's retirement and wrestlers in MMA watch video >>
Gerald Brisco on wrestling, MMA and WWE watch video >>
Pete "Drago" Sell looks forward to his MMA return and hopes to complete the road back to UFC. watch video >>
The pro wrestling champion discusses his work portraying a fighter based on Fedor Emelianenko in the mixed martial arts movie "Warrior" from Lionsgate. watch video >>
The new power couple of MMA, Jorge Gurgel and Zoila Gurgel (formerly Frausto) talk about current events in MMA as well as the married life. watch video >>
The former titleholder of Bellator Fighting Championships' lightweight division looks forward to an April rematch with Shinya Aoki. watch video >>
9-11 & NY MMA: A Fighting Spirit. A short film by The Fight Nerd, Ranger Up and Kahl-One Productions. watch video >>
Muay Thai trainer Mark Dellagrotte talks up the upcoming UFC Trainer game. watch video >>
NY MMA director Kahleem Poole Tejada talks about his documentary watch video >>
Bellator middleweight champion Hector Lombard dismisses criticism of his win on Saturday over Falaniko Vitale. watch video >>


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October 31, 2006
Joint Statement From The George Washington University
and the Bazelon Center for Mental Health Law
Regarding the Lawsuit by Former Student Jordan Nott
The George Washington University and one of its former students, Jordan Nott, today announced that they have reached an agreement to resolve the lawsuit filed by Mr. Nott last fall against the University and several other entities regarding his October 2004 mental health hospitalization, the University's interim suspension, and his subsequent withdrawal from GW.
"I hope that this difficult experience will result in positive changes in how student mental health issues are handled at campuses across the country," said Mr. Nott. "And I certainly hope that other universities will not discipline their students for seeking mental health treatment."
"This was a very difficult situation. We were attempting to serve the best interests of Mr. Nott while also considering the well being of all of our students," said Dr. John F. Williams, GW provost and vice president for health affairs. "While we recognize that some steps in the process may not have been perfect, we stand by the result. We appreciate Mr. Nott's support in resolving this matter, and we wish him continued success."
"We are pleased that GW is reviewing and revising its policies, and hope that Jordan's case and others will prompt universities to adopt policies that do not penalize students for seeking mental health treatment and that are not linked to the disciplinary process. It is important that any policy provide for individualized determinations of whether a student can remain on campus based on an objective medical evaluation after discharge from a hospital, without rigid rules based solely upon the fact of hospitalization," said Karen Bower, from the Bazelon Center for Mental Health Law, one of Mr. Nott's attorneys.
All parties stressed that students should seek professional help in times of need. The University Counseling Center, Student Health Services, GW Hospital, and referrals for other services are indicative of the types of services available to GW students. Every year, GW's University Counseling Center provides services to hundreds of students, helping them to remain successfully engaged in their school.
All parties have agreed that the terms of the settlement are confidential. Mr. Nott was represented in his case by the Bazelon Center, and two private attorneys, Stephen Chertkof and Allen Bachman.
For more news about GW, visit the GW News Center www.gwnewscenter.org.
Tracy Schario: 202-994-3566; firstname.lastname@example.com
Matt Lindsay: 202-994-1423; firstname.lastname@example.com
Lee Carty: 202-467-5730 x 121; firstname.lastname@example.com
Karen Bower: 202-467-5730 x 132; firstname.lastname@example.com
The George Washington University Office of University Relations,
Washington, D.C.


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ED Magedson – Founder
Ripoff Report
Spirit @ Guild,4414 Excelsior Blvd Edina, Minnesota USA
Spirit @ Guild, Marilyn Non payment for merchandise order and delivered Edina Minnesota
Does your business have a bad reputation?
Fix it the right way.
Corporate Advocacy Program™
As an indepenedend sales representative, I received two seperate orders from the owner, Marilyn, for merchandise to be sold in her store Spirit @ Guild. The first order was for jeans from Denimocracy. After chasing her for payment, she sent a check for $1,372 (check # 3907. dated 10/17/2013) that bounced. She has avoided all attempts to contact her.
The second order was for knitwear from Bodyfit for Afra. Marilyn never made an effort to pay, and has avoided all of our efforts to settle this.
This report was posted on Ripoff Report on 04/07/2014 11:12 AM and is a permanent record located here: http://www.ripoffreport.com/reports/spirit-guild/edina-minnesota-55416/spirit-guild-marilyn-non-payment-for-merchandise-order-and-delivered-edina-minnesota-1136928. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
Ripoff Report has an exclusive license to this report. It may not be copied without the written permission of Ripoff Report. READ: Foreign websites steal our content
Click Here to read other Ripoff Reports on Spirit @ Guild,
If you would like to see more Rip-off Reports on this company/individual, search here:Search Tips
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|Submitted By:
|Matt McMurray on Aug 15, 2006
|Mount Charleston Wilderness Area MORE INFO >>>
|Topic Title
|Mt. Chaleston Camping
|2 days ago
|Partner wanted 6/2 & 6/3
|2 days ago
|Climbing partner 06/10/17 - 06/14/17
|Jonny Allen
|2 days ago
|re: Tom Beck RIP
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|2 days ago
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|3 days ago
|re: Am I mad/stupid for wanting to climb something big in RR in July?
|Guy H.
|3 days ago
|Las Vegas Partner Wanted June 4th Through June 7th
|Francis Davis
|3 days ago
|re: Las Vegas Climbing 5/19-20
|SpencerB BB
|May 18, 2017
|Comments on Mount Charleston
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|Show which comments —
By Dirty Gri Gri, or is it GiGi?
From: Vegas
Oct 3, 2006
Campgrounds of Mt. Charleston: (dead link removed)
The 'Hilltop' is the most popular campground, and has warm showers (coin operated). Make your reservations way ahead of time, if planning on being there in the summer/warmer months.
By Mike Dudley
From: Vegas
Jun 22, 2009
|There are not that many routes listed here on MP but there are a lot of routes up there. Grab a guide book and enjoy the super hard and sharp limestone!
By bosworth
Oct 5, 2010
Keep an eye on the fixed link draws in the area, especially at crux's. After taking a couple of good sized falls on the crux of The Great Train Robbery, (the bolt below the ledge), I managed to core shot my rope on a carabiner that had been worn down enough to create an edge. I have replaced this biner, but that is not to say that it won't happen again or that there aren't more biners such as this one.
I would like to stress the importance of keeping your rope on a rope bag during belays as to keep dirt and debris from wearing down the surface of draws. It was kind enough of our fellow craggers to put these permanent fixtures up, but it is our job to keep them safe.
By Rod J.
From: Bend, OR
Mar 30, 2016
I kept hearing that Mt. Charleston was North of Las Vegas, so I thought it was up highway 93. Turns out that it is due (magnetic (+11 deg.)) West of Las Vegas.
Another way to look at it is that if the North portion of highway 215 were to continue West, it would pass North of Mt. Charleston!
Hope this saves somebody a trip up highway 93.
By Adventure Chumps
Apr 6, 2016
I'm guessing this was you as well then?
By Sam Thompson
From: Missoula, Montana
Jun 10, 2016
|soul train is 5.13d fyi
By Brandon.A.T.
Jul 10, 2016
|A lot of people have told me this area is extremely sand bagged. I just got back from my first trip here and would have to disagree with that idea. I generally climb well featured high friction overhanging sandstone so making the transition was definitely difficult for me. But once I figured out how to climb the limestone here the grades felt in line with most areas I have climbed at. It was also interesting to find that the harder the grades got the better the holds were! This is a great summer climbing destination that I highly recommend to anyone climbing at least llc and above. While Vegas is 120 degrees out Mt Charleston is in the 60-70s and shade can be found! If you do climb here be prepared to become a better rock climber as most of the routes 12 and under demand flawless technique. My only complaint is that while the bolt spacing is great the bolt placement can sometimes be annoying. A few routes would have a bolt in the middle of a crux instead of near the jug's before and after the crux.


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What exactly are the criteria used when determining which and how many AV-programs are installed on a machine?
We use F-Secure Anti-Virus which in the case of version 5.xx installs 3 programs (versus 2 with version 7.xx) listed under installed programs. Only one of these is the actual anti-virus utility while the other two are used for downloading updates and management in support of the AV-util.
Both version trigger an alarm.
Is there anywhere I can tweak this alarms behavior?
-Roger A
2 Replies
· · ·
Aug 14, 2007 at 3:14 UTC
Sorry - there is no way for you to tweak this currently. We are working on greatly improving our av detection in our 2.0 release due in a couple of months.
· · ·
Aug 15, 2007 at 4:23 UTC
Sounds fair enough. Looking forward to 2.0!
-Roger A


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Dan Deacon Coming Back to Atlanta October 2 to Play Eyedrum
Fun-maker extraordinaire Dan Deacon will be returning to Atlanta to play Eyedrum on October 2. Some may recall that Deacon's last show in Atlanta was also scheduled to play Eyedrum, but once the show sold out the promoters moved the gig to the larger Masquerade. We're expecting another huge turnout for the show this time, though expecting it to stay at Eyedrum, which will hopefully help with the venue's recent money woes. So do you part to give some cash to a revered Atlanta venue, which also showcases some awesome experimental music among many other genres, and get a heckuva party in return. Tickets just went on sale at Ticket Alternative, so grab them while you can.
Category: Gigs


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An Sen ClinicAcupuncture
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Motorcycling is becoming more popular for women and there is an ever increasing number of female motorcyclists taking to the road. Spidi offer a huge range of motorcycle clothing for ladies, which has been specially designed to suit the female form. Being Italian, Spidi understand the importance of style and the Spidi Fit For Lady collection includes a great range of stylish items that incorporate the best protection and features.
Spidi offers an extensive range of ladies suits, jackets, trousers, rainwear, gloves, protectors and base layers to suit all tastes. So whether you like the racing style, touring or urban riding, you can be sure that Spidi will have a top end product for you.
The Spidi ladies motorcycle clothing includes CE approved protectors, high tenacity fabrics and Tenax Flex areas for stretch, comfort and perfect fit. There is a range of Fit For Lady jackets and trousers featuring the Spidi H2Out membrane which ensures the items are waterproof, windproof and breathable.
The Spidi range also includes a selection of items in both man and lady fit for those couples who would like to coordinate on the bike. Ranges include the Spidi Venture, RR Leather and the Spidi Trackster Touring suit.
We think the Spidi brand is one of the most stylish and extensive out there and with the highest safety features and performance the Spidi ladies motorcycle clothing is a top choice for female motorcyclists.


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Back in March of last year, Google introduced a ridesharing tab into Google Maps, allowing anyone to see potential pricing and timing for ordering an Uber or Lyft ride. Today, Google is introducing a streamlined experience, bringing the ability to book those rides directly from Google Maps. (more…)
Uber Introduces Redesigned App
Uber dropped a new redesign on us this morning, one that flips the experience a bit by starting you off with a question, "Where to?" That's in a way Uber realizing that when someone requests a ride, the whole point is that they have a destination in mind and would rather tell Uber where to take them upfront instead of telling it where to pick them up. That make sense? (more…)
Pittsburgh Residents, Uber is Rolling Out Self-Driving Cars
Don't get me wrong, Pittsburgh is a fine city, but did anyone think it would be the first area you can take a self-driving Uber from place to place? Well, starting this week, Uber is rolling out self-driving vehicles for public usage, providing the same experience as an UberX.
According to Uber, these vehicles will only be usable by Uber's most loyal riders, and I have no idea what that actually means. While I don't think Uber knows if you have ever used a Lyft before, anything's possible. My guess is, if you find yourself taking Uber quite frequently, and you're in the vicinity of one, you may see it pop up inside of your Uber app. (more…)
Google Maps Gets WiFi Only Mode, Offline Maps to SD Cards
A Google Maps update will start rolling out to Android users today that includes a WiFi only mode and the option to save offline maps to microSD cards..
Google walked through a bunch of scenarios specific to each feature in today's announcement post, but this stuff is really pretty self explanatory. In WiFi only mode, you get to tell Google Maps to only work when connected to WiFi. That way, you can save on data consumption and use Maps offline. The added bonus here is that you are basically putting Maps in airplane mode, while the rest of your phone stays connected. Sweet, right? (more…)
Uber Introduces Scheduled Rides to Let You…Schedule a Ride
If there is one feature that Uber has desperately needed since launch, it would be the ability to schedule a ride. The thought here is that you probably know ahead of time that you'll need a ride, but you don't necessarily want to deal with last minute requests and would instead like to set something up ahead of time, you know, to cross it off the list of "Sh*t to do before my trip."
Today, Uber is introducing Scheduled Rides globally. As the name suggests, you can now schedule a ride with Uber anywhere from 30 minutes to 30 days in advance. (more…)
Uber Revamps In-App Customer and Driver Support
Since the beginning, customer support on Uber has equated to only emailing its support teams across the country, but going forward, things are going to change. With the company's latest announcement focused entirely on customer service, whether it be for drivers or passengers, many of the help you might need is no more than a few taps away.
Instead of emailing messages to teams, you will handle claims from within the Uber app, thanks to an updated Help menu that will guide you on which steps to take in order to resolve any issues you may have. (more…)
Report: AT&T Might Sell a Bloatware-Infested Cyanogen-Powered ZTE Phone
According to a report out of The Information, AT&T is looking into a Cyanogen-powered phone, because it thinks that Cyanogen could make a compelling Android-alternative option to its customers. That may be better put as, AT&T thinks that by partnering with Cyanogen, it can load up even more garbage bloatware on phones to help push you into their other services, like DirecTV. (more…)
Uber Intros Family Profiles, Pay for Your Kid or Friend's Ride
Today, Uber introduced Family Profiles, the easiest way to make sure your children, friends, and loved ones are getting around safely through the city.
When a Family Profile is created, multiple users can request rides from the same account, with Uber billing a single payment form. For example, if you have a kid in college in a different city, that child can request an Uber and pay for it using the Family Profile (mom or dad's credit card), instead of using their own money. (more…)


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Vocalists from the Marble Falls High School Choir Program competed in the University Interscholastic League (UIL) Region Solo Contest at Stony Pointe High School in Round Rock last Friday, February 19th.
"We had 50 soloists compete," said High School Choir Director, Bryce Gage. "43 received a Superior rating of 1. 23 of those soloists qualified for State Competition!"
The State bound students will compete on Memorial Day in May at the University of Texas in Austin. The extra work, practice, and time put in outside of normal school rehearsals by the student performers helped impact the superior results. Gage reports that all of the MFHS competing soloists study privately with one of program's voice teachers: Sarah Spillman, Susan Ohlrich and Deborah Vanicek.
Second Group of MFHS Superior Rated Choir Soloists
"Our kids performed at such a high level, they represented Marble Falls ISD extremely well," said Gage. "The UIL Solo Contest is a stressful competition. The students walk in for a judge they've never seen before and since its UIL anyone can be in the room. I am very proud of how they performed!"
Learners Today, Leaders Tomorrow….Mustangs Forever!
Contact: Bruce Peckover
Director of Communications


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Category:Rye House, Hertfordshire
English: Rye House was a building in Hoddesdon in the English county of Hertfordshire.
- Main Wikipedia article: Rye House, Hertfordshire
This is a category about listed building number 1341877. (More images)
Media in category "Rye House, Hertfordshire"
The following 17 files are in this category, out of 17 total.
- Rye House 1824 Cooke.jpg 135 KB
- Rye House EH New.jpg 149 KB
- Rye House EM 1805.jpg 107 KB
- Rye House Plot.jpg 144 KB
- Rye House TC Dibdin.jpg 75 KB
- Rye House Vince 1881.jpg 39 KB
- RyeHouse2.jpg 148 KB


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- Offer two or more courses each year specific to Native American studies
- Renew the Native American Student Organization
- Support research by providing travel scholarships and research assistantships
- Host an annual speaker in Native American studies
- Leverage existing USA resources through the Archaeology Museum and the Marx Library to promote awareness of Native American issues
MOBILE, Ala., March 7, 2014 /PRNewswire/ -- The University of South Alabama and the Poarch Band of Creek Indians have announced the creation of a Native American studies program that will focus on the history, culture and modern life of the Tribe, along with others in the Southeast and across the United States. The Poarch Band of Creek Indians Native American Studies Program will be funded, in part, by a $500,000 gift from the Escambia County, Ala., Tribe. "We are thrilled to make this opportunity available to USA students so they will be able to further their education in Native American studies," said Buford L. Rolin, tribal chairman of the Poarch Band of Creek Indians. "The Tribal Council realizes how important quality education is, and for students to have the opportunity to learn about our history and culture at the University of South Alabama will only enhance the current studies being offered by the University." "This gift represents a substantial investment in the preservation of Native American heritage and culture," said Dr. Joseph F. Busta Jr., USA vice president for development and alumni relations. "Together we are celebrating a historic step in a relationship that will improve the community's knowledge of the important contributions the Poarch have made to this state's history and culture, and enrich the education of generations of students at South Alabama. We are proud that the Poarch Band's tribal leaders have chosen us as partners in this important effort." The philanthropic support will provide funding to the USA College of Arts and Sciences for an endowed professorship and program support. Among other activities, the University will: