text:
Current versions:
With a few clicks in the software SFKarte you can create your own personal greeting card.
No matter whether you need greetings, congratulations - or thank you cards, with the software SFKarte you can make easily and quickly according to your wishes and ideas. Even creating documents is part of the Repertoire of the software. Then you can print your created map easy and convenient. Best you have a color printer for this. The software SFKarte gives you seven different map sizes, such as for example DIN A4, DIN A5, DIN long, etc.). Are you also in the software SFKarte to the 200 background patterns, as well as various design templates available. If you want to, you can upload also clipart images in the software SFKarte. The operation of the software SFKarte is very user-friendly and self-explanatory. But also various help topics to choose from are you if you no longer know or questions arise.
The software SFKarte allows you to import selected images in the format BMP or JPG. Thus, you can add a personal touch to your card. In the software SFKarte are numerous positioning AIDS available, and using the preview function, you can, at any time take a look at your map. Your text, you can create and write. You can use several options like for example shades, rotation, transparency, etc. in the software SFKarte to make your card and the test. Finally, you can export your finished card as a JPG image file in the software SFKarte.
You can download the trial version of the software SFKarte you on our Web pages, where the trial version only for a certain period of time available to you. You can buy the full version on the website. Thereafter you will receive an unlock code, as well as a CD by mail delivered.
Functions of the software SFKarte
• Picture import of JPG and BMP
• Design templates
• 200 Background pattern
• Positioning AIDS
• Preview function
• Export option as a JPG image file
The software manufacturer information
The company SF software headquartered in Marktoberdorf - Biel behind Frank Scholzer, who mainly software designed to make life in the Office. So arose to such products for the execution of service plans, business cards design, calendar, folder lable, etc. where the company with the software SFKarte has also thought of private people.
With the new version of SF card, you can create your own greeting cards on the PC even easier and better. Currently, you can use 7 different sizes of cards and 200 different background images. The design of the cards is very easy by the graphical user interface, and your cards are quickly created for every occasion. Graphics, you can easily import and position on the map and freely enter texts. The card is then printed or exported as a .jpg file.
Features of the current version 4 of SF map
• 7 different card sizes
• 200 Background pattern bootable
• Color overflows image patterns, shades, transparency framework, arbitrary rotation
• Picture import of JPG and BMP
• Export option as a JPG image file


text:
Against all odds, Katniss Everdeen has survived the Hunger Games twice. But now that she's made it out of the bloody arena live, she's still not safe. The Capitol is angry. The Capitol wants revenge....
Still hungry? Listen to more Hunger Games.
©2010 Suzanne Collins (P)2010 Scholastic Audio
"At its best the trilogy channels the political passion of 1984, the memorable violence of A Clockwork Orange, the imaginative ambience of The Chronicles of Narnia and the detailed inventiveness of Harry Potter." (New York Times Book Review)
"Unfolding in Collins' engaging, intelligent prose and assembled into chapters that end with didn't-see-that-coming cliffhangers, this finale is every bit the pressure cooker of its forebears. [Mockingjay] is nearly as shocking, and certainly every bit as original and thought provoking, as The Hunger Games. Wow." (Los Angeles Times)
"Fans will be happy to hear that Mockingjay is every bit as complex and imaginative as Hunger Games and Catching Fire." (Entertainment Weekly)
"Loved the ending!"
Loved that I new that Katniss and Peta had to get together!!!
When Katniss shot the President of District 13.
The whole story moved me!!!
"Unexpected Storyline"
I would not consider this to be the case, because you can't just skim over the parts where Katniss is whining.
I was disappointed and felt as though there was no true conclusion; things abruptly ended.
The reader was fantastic, but this story was nothing like the first two. Katniss just came across as a whiny little girl, and while I understand that she has been through alot, that is not what I want to hear about. I wanted action like the first two novels, and got alot of drag in the plot.
ADD Diva
"A children's book? REALLY? NOT!!"
I wish that I would have not listened to the hype about this trilogy.I wish that I could erase the fact that I read/listened to them at all. I wish that I never bought them at all.
Hunger Games was the tamest of the three, but all of them detailed starvation, deranged so-called "leaders" who were just plain SICK in all ways imaginable. This book and the trilogy in general just left me with a sick feeling in the pit of my stomach. That the Hunger Games was filmed in my state just made it all that much worse. It has been a really long time since a work of fiction has made me feel so much rage for the characters. I felt rage at the injustices thrown at them, the abuse heaped upon them, and the games that these kids were subjected to. **Sigh** How it was classified as a children's book totally astounds me. Do NOT read this book if you have ever suffered any sort of abuse in your life, as triggers are written all through the 3 books.
"A great ending"
It was a great story. I really cared about the characters.
The ending was so sad.
I loved the commentary at the end of the story.
"Gave into the hype...VERY DISSAPOINTING!"
the lead character is whiney, selfish and annoying. I loved the concept of the future USA in this book and the hunger games, etc...but I think the book "jumped the shark" when it introduces the mutt/wolves/dogs/dead character/being things. When they were introduced I exclaimed" ARE YOU SERIOUS! THAT IS SO DUMB!" I listened to all the books, hoping it would get better, the main characters would mature... to no avail. Thats what I get for reading/listening to a book with teenage characters...I cant relate...
I did like the narrator.
"Overall very entertaining!"
Yes sure it was a good listen, wraps up the story of catness quite well.
Excellent reader, really helps you get into the story.
"in the future, girls are on fire..." jk i don't know.
"Readers voice"
I loved this book, the story was great! The voice of the woman who read it however, was a different story! Her voice was grating and she made Katniss sound like an overly anxious and awkward girl instead of the strong, brave, even though sometimes desperate girl I had imagined. I'm not sure if the tinny sound of her voice was due to poor quality of the recording or if it was the woman's voice but it was irritating to the point that I wasn't sure I would actually finish listening to the book.
"Reading superb, story a let down."
Yes .
I would change nothing about the reading. Carolyn McCormick was so good that when I tried to read the book myself I would go too fast to get as into it. Her pace and skill at dramatic reading takes the reader through every moment. So listening to her really maximizes the experience.
As for the story itself: the things that "worked out" in the end did not make the agony worth it. The author cheated us out of satisfaction. I can respect her realism and avoidance of a typical "happy ending," but something was missing. I think Collins could have at least given us a good couple chapters on the reconciliation and love between Katniss and Peeta.
"Fast paced, exciting, but shallow"
This story drew me in with tremendous force. I couldn't put it down
I enjoyed the fast pace and powerful story but wanted more in terms of character development.
"Great book in a great series."
As good as the first two books.
Have had a hard time limiting myself to only listen while on a trip.
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subset: commoncrawl


text:
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Located just 1.3 mi (2.1 km) from the airport, Skianthion offers a shuttle (available on request). Free perks include WiFi and self parking. Other highlights include a garden, and rooms offer LED TVs and refrigerators.


text:
The REAL January 11th- The 2009 Protagonists' Diary
Dear Diary,
I feel the need to write in here a bit earlier than predetermined. Reasons why I am so anxious to share my random thoughts of the new year? Well, the moment that TheRiverTalker asked me to write in here I was all giddy with nerves, not going to lie, but I feel more anxious over the fact that he is giving me this opportunity over anything else, considering that he is one of the most revered writers on Protagonize. So without much adieu Diary I write to you, in terms of a poem:
Every morning I awake,
To opportunities I must take.
My school is currently on a strike,
And that's somewhat okay, I can still type.
When I was younger and I found this site,
I knew it would be something that would affect me, for the rest of my life.
I would write and write, hoping to catch some attention,
But I would barely notice the amazing writers who made this place so full of affection.
Back then I was a fool, who only cared only for ratings,
But now I write my words without hesitating.
This new year I have made new friends,
Made new ideas for everyone to attend;
I have perhaps found future novelists who will one day,
Write novels, that in my curious hands may lay.
For this years resolution,
I have come to the conclusion,
That I will reflect on every writer to catch my eye
And a bond with them and their writing, I will tie.
Dear Diary I say to you clearly,
I used to be a selfish writer, who, about their writing, cared too dearly,
But starting this year,
I tell you all here,
That since I joined this family,
I matured into a writer happily.
That is my New Years resolution Diary, I hope that I can fulfill what I propose, though I started that a while back before the new year. I now pass the next day in the new year to the next entry, good day and farewell Diary.
Daisuki Desu,


text:
Dozens of people are thought to have frozen to death in extreme conditions across Europe.
• In Poland nearly 50 people, mostly homeless, have frozen to death in December as temperatures dropped as low as -18C, according to local TV. Police said 15 people died on Saturday.
• Ukraine reported 27 deaths while six people were killed in Germany where temperatures of -33C have been recorded. Düsseldorf International airport was closed. Airports in the Netherlands and Belgium were also affected.
• Forty per cent of flights have been cancelled from Paris, while snow covered the beach in Nice. Minimum temperatures reached -23C in some parts.
• In Spain runways were closed at Madrid's Barajas airport after temperatures fell to -8C and high-speed AVE trains were suspended between the capital and Barcelona, Malagá and Seville.
• More than 220 villages and towns in Bulgaria were left without electricity, and toppled trees cut power in several mountainous suburbs in the capital Sofia.
• An overflowing dam in the town of Zlatograd, also in Bulgaria, flooded the basements of several apartment buildings, the town's mayor told news agency Focus.
• Some 50 passengers were injured early today in Croatia when a brake failed in temperatures of -17C on a train, causing an accident at the main station in the country's capital, Zagreb, police said. One person was critically injured.
• In Moscow, where temperatures fell to -26C last week, relatively warmer temperatures of -13C brought heavy snow falls in the Russian capital, blanketing Red Square and the Kremlin. Up to 9,000 snow ploughs were said to have been sent out to clear the streets.


text:
The UnthinkableMature
I was walking back home from college after a long day at around 6 pm, I always take a shortcut where I walk in a back ally that is always empty. As I was walking a van entered the ally and was coming my way. The strange van pulled beside me and it's door was opened, 5 seconds later I was in the van with a hand over my mouth sealing it and the other hand was keeping me from moving, they were the hands of a strong man. My eyes came to focus and infront of me was a woman with a beautiful long black hair, blue eyes and perfectly oval face. I think she was around 26 years old. She looked at me with a serious face and said with a clear voice "Don't struggle or do anything stupid and you might make it through the day".
My whole body started shaking, to her left sat a girl with a blond ponytail hair, blue eyes and a fine featured face. I guess she was 18 or 19 years old. She looked heavy-hearted with sad expressions. Did they kidnap her too?
As I was trying to take the whole thing in the woman spoke again "I'm sure you are confused about this so let me explain things, we are a part of an organization called Eclipse and we need your brother's help. We want him to work on some nuclear experimentations and applications. Your brother has been retired for a while now so the surveillance the CIA put on him is no more. If your brother doesn't cooperate he'll lose everything precious in his life"
As she said these words she seemed dead serious. Then a man spoke from the driving seat, I couldn't turn to see his face. "Why do you even bother explaining, after all he saw our faces".
I flinched as I heard these words. They could only mean that they will kill me before long. Everything went silent for a couple of minutes and every single bad outcome and scenario rushed into my mind, I was overwhelmed by the situation.
Then the girl next to her looked at me and started to speak "I'm sorry this is ha.."
"Shush Elizabeth" the black haired woman stopped her. "You don't apologize to people who's lives you are about to ruin. Actually, you don't apologize at all."
Elizabeth looked away and said "Is this who we really are now?" in a sad and deep voice.
The man who was gaging my mouth with his hand finally spoke "Welcome to the real world kiddo".
I struggled trying to speak but all that came out was "mm mmmm mmmm"
The black haired woman looked at me and said "You have something to say? Ok but if you even think about screaming not only you but your brother and his family will pay the price. Am I clear?"
I nodded. Then she signaled to the man behind me with her head and he removed his hand from my mouth. I had to think carefully about what I say but I was in no condition to think clearly.
I gasped for air a little then I spoke "Please don't hurt my brother, I'll do anything you want just leave him alone, he retired because he didn't want to work on any nuclear projects anymore, he had enough. He will never agree to help you."
The woman looked at me and said "He will help us if the life of his family is at stake, just like you are willing to do anything so he won't be put in that position." She smiled a little then said "You really love your brother huh? Don't worry, as long as he does what we tell him to do everything will be just fine."
Elizabeth looked at me with a tear in her eye. Could it be true? Would they really let us all live if my brother does what they ask him to do? Or is every god damn word that comes from this woman's mouth is a lie? I asked a question. "What kind of nuclear stuff are you going to force my brother to work on?"
It was the man who was halting my movement that answered "We don't ask questions, we get the job done. Now stop with the stupid questions before I lose my temper."
He's right, it was a stupid question. They were men for hire, it was obvious from the moment they picked me up. All I really need is an answer to the final question, the one that really matters.
"What if my brother refuses to help you, even if at the cost of his family's life? What will happen?"
The lady in front of me answered "Lets hope your brother isn't brave enough to find out." There was something in her eyes as she said that, it's like she was hoping it doesn't come to that. I was glad to see that.
Then the man behind the wheels said "We'll be there in a couple of minutes" then the woman shouted "Ok put on your masks, we go in grab the target and go out, it's a simple and smooth operation so don't screw this up." She looked at Elizabeth "You're gonna be ok?"
Elizabeth took a deep breath then answered "yeah, I'm ready." The black haired woman patted Elizabeth's back and said "Come on... you've been through worse. Don't worry, it will be over before you know it".
Then they all took out red masks that had the shape of a dragon, under different circumstances I would've said they were awesome, they had a very strange yet great looking design that you could tell it was well thought of.
I was restless, thinking so hard of a way out of this because I knew that everyone was in the house now. They probably have been watching us and picked the right moment, but I can't think of anything I can do to stop this, it's hopeless. Our house was alone on top of a small hell so screaming for help there wouldn't help. As I was thinking the car stopped. They picked up rifles from a bag and the man behind me wrapped a piece of leather around my mouth, then the black haired woman opened the door and we all stepped out and ran towards the house, now I couldn't see face of the man who was driving or the one who was holding me in the car because of the masks but both of them had muscular bodies, probably ex-military.
As we reached the house door the driver kicked in the door and went inside first, we followed him to find Matt and Sarah sitting in the living room watching TV.
Sarah started screaming as Matt was lost in confusion from what his eyes were seeing. A few seconds later the black haired woman was putting a gun in Sarah's face as she told her to shut up or she dies.
Matt started shouting "leave her alone! Who are you people? What do you want from us?" then he finally noticed me "Drake? Oh my God! Drake are you ok? Who are these people?"
Then the black haired woman screamed "shut up before I lose my temper and shoot her, calm down and allow me to explain but before that, where is your son?"
the question scared him, did they come to take his son? "he's... sleeping upstairs" he replied.
She went on "I'll be brief so listen carefully. My name is Jennifer and I work for a mercenary organization, You're going to come with us to work on some experimental nuclear tests, we'll explain everything on the way, if you refuse we'll kill you all right now."
My brother was silent for a second, not knowing what to say or do. Then he spoke "I've retired long ago, I don't work on anything that has to do with nuclear energy anymore" Jennifer was angered by his response and shouted "you think I'm joking around? Fine" then she removed the safety lock for her rifle, aimed at Sarah's face and put her finger on the trigger.
My brother shouted "Stop please! I'll do it, I'll do anything you want… just please, please don't hurt my family" he knelt on the ground. As I was watching my brother, the man I treasure the most in life, beg for our lives everything in my mind went blank. There was a way out of this but it was something I would never do but as all reason and thought in my mind became void I was only left with instinct, and then it happened.
I noticed Elizabeth who was standing next to me with her gun held and pointed at my brother, her body was shaking and she was holding her gun with a weak grip, the man who was holding me captive the whole time had let his guard down ever since we entered the house. So there was my chance and I took it.
I ran towards Elizabeth as fast as I could and took her gun away from her then I pointed it, removed the safety and pulled the trigger… A gun shot was heard… I had done the unthinkable.


text:
Jamie Estrada, who served for a few months as Martinez's campaign manager in 2009, was originally indicted in May on 12 counts of intercepting emails on the campaign account and two counts of lying to FBI agents during an interview.
Estrada, who pleaded not guilty to the original indictment, has sought to have the original indictment dismissed because it charged him with intercepting "wire" communications.
The new indictment charges him with 12 counts of intercepting "electronic communications."
The intercepted emails were distributed by Martinez's opponents after her election to various media outlets. She asked for a federal investigation after the emails surfaced and said she had never seen the emails sent to her by supporters and in response to some personal purchases she had made online.
Estrada is charged with hijacking the email domain name and redirecting it to another account that he controlled.


text:
STRANGE Wiper issues!
I've owned a few Wranglers...usually older models. I could find my way around them pretty easily.
Now I own a 2008 JK, and I can't figure this one out.
Here's the issue:
I went on a small road trip (50 miles) and it drove fine. Parked the car. A few hours later I go to start it up and the wipers are going full speed with the switch on "off", and the motor is acting funny. I then turned the wipers to intermittent, and they stopped...and the engine bogged down and quit. I ran it in idle today to test it, and like yesterday, it will stay running so long as I have the wipers set to "off"(but they are still wiping...go figure). As soon as I turn the wipers to intermittent, the engine bogs down like it's running lean and the wipers die. At the same time, when I turn the switch back to "off", the wipers come back on, and the engine comes back to life (though running a little rough still). AND, while listening to the idle of the engine with the wipers off (still wiping of course), while the wipers make a pass one way across the windshield, the engine will start to die, but when the wipers are passing back, the idle goes back to normal. I am getting error codes of P0171 and P0174 (lean mix), which I suppose are caused by the wiper malfunction.
I've never seen this before, and I am at a loss. Anyone know how to fix this issue?
Uhmmmm...loose battery terminals..bad computer......bad ground at battery. Messed up bcm.......wow.....I wouldn't even know how to troubleshoot that and I've worked on vehicle electrical systems for the last 10 years! (up until about a year ago)
I would also say most likely bad ground. The wiper park switch needs a ground to park the wipers. Eng functions also rely one good grounds.
Disconnect the wiper motor and see if that narrows down the problem. Dodge had a problem on Rams (and other vehicles( with the wipe stalk switch. The switch, via the instrument cluster and TIPM control the wiper circuit. The stalk switch was $100, the motor $50 to $100 depending on sourcing. I'd start with vehicle grounds and disconnect the motor. Clear the codes and see what happens.
I'm going to try disconnecting the wiper motor. I've cleared the codes already, but I'll do it again when I check the motor grounds. I'm pretty convinced it's the motor fouling up or the grounds to it. I can't see what else would drain the engine like that.
That happened to me twice. Flash update fixes that.
Well, I disconnected the wiper motor and........no start.....nothing. I plugged the wiper motor back in, turned the switch to off and it started. It's either a cross somewhere in the loom, or the computer is glitched. Maybe I should flash it, as I was told. I even had a dealer mechanic look at it and he was clueless.
Why does it have to be me!!! Somebody just shoot me now!
Well...found it. The engine and the wiper motor share the same ground. Ground was fixed and cleaned and the things runs great again ($250 later). Hopefully this experience will benefit others on this forum.:thumb:
Thank you Bill.
Here's another strange wipper problem. I posted this same entry on another post where we are talking about TIPM issues, but thought you may be interested. I'm still wrestling with my problem. Here is my story:
One thing i have on my 2008 JK wrangler is a loose positive post connector going into my TIPM.
I can wiggle the post a bit which means whenever i hit a bump or shut my door, or just bump my vehicle a weird thing happens.
My wipers go on.
My washers go on.
My horn goes on.
The only way i can stop it is to pull over, open the hood and smack the top of the TIPM.
I can reproduce the effect if i just wiggle the wire that goes to the positive post connector.
I've tighted down the bolt, but it appears to still be loose. I would really like to get some help to tell me if i need to get a replacement TIPM, or if i could somehow fix this.
it's scary just getting in my jeep, since at any time driving or just standing still, the S**T may start going off.
help is appreciated...
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text:
PLANS to extend the requirement for litigants to apply for leave to appeal to the Court of Appeal and cut back on the number of oral applications have been announced by Lord Woolf as part of his self-styled "blitz" on court delays.
The judge outlines the proposals, including a pilot under which only one, rather than two judges will hear certain leave applications, in his annual review of the Court of Appeal Civil Division's legal year.
The proposals come hard on the heels of his decision to appoint 12 up-and-coming law-yers as judicial assistants to help judges deal with the appeals backlog – there were 1,384 cases waiting to be heard at the end of 1996, compared with 672 at the end of 1992.
In his report, Lord Woolf argues that the rules governing leave to appeal are too complex and place "a huge administrative burden on court staff". He adds: "There is an overwhelming case for extending the requirement for leave to all appeals other than limited, easily defined categories of appeals because of the reduced burden this will place on the staff of the court who can then be deployed on more productive tasks."
Woolf also announces the pilot under which only one judge will hear applications "where that judge does not consider that the application needs to be heard by two judges". He adds: "Consideration will also be given to dispensing with oral applications where the court is of the view that an oral hearing will not assist."
The proposals will be submitted to the court's users committee for consultation. Committee member Suzanne Burn, who is secretary of the Law Society's civil litigation committee, said it had not yet considered the proposals. She warned that any extension of the requirement for leave to appeal should be undertaken carefully or it might lead to ever longer delays.


text:
- Daniel de Vise
- Reporter
Daniel de Visé is higher education reporter at the Washington Post and author of the College Inc. blog. He has worked as a journalist for 20 years, including stints at the Boca Raton News, Long Beach Press-Telegram, San Diego Union Tribune and Miami Herald. He is a graduate of Wesleyan and Northwestern Universities.
E-mails show U-Va. board weighing move to oust president
"I expect to be bullet-ridden," Helen Dragas told a board member in newly-released email messages.
Report finds for-profit colleges serve shareholders over students
A two-year investigation found that half of students who enrolled from 2008-09 left without a degree, and that for-profit colleges are better at recruitment than retention.
No 'magic solution' to college affordability
Two factors, high labor costs and competitive discounting, drive up the sticker price of college, the guest blogger opines.
U-Va. deans hatched online plan the day Teresa Sullivan was asked to leave
Neither Sullivan nor Rector Helen Dragas knew how close the institution stood to joining Stanford University's mass online partnership.
- U-Va. parent: Online learning is an oxymoron
- Fixed-rate tuition can minimize college-price angst
- U-Va. leaders appear not to have known of looming online deal
- U-Va. takes major step in online education
- St. Mary's College moves to shore up selectivity
- Sullivan: U-Va. more united than ever
- A pragmatic liberal arts education
- Five college admissions tips for the A-minus student
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text:
change of plans for lifeMature
well just thaughts i get and when i get them i write em quick and publish them so excuse any miss spelled words or bad grammer, thanx xoxoxox
There comes a time in life where you realize that you have to better then you are because, you're not actually getting anywhere by continuing with your ordinary life. When this feeling comes what do we do? Well I'll tell you what we do, we get of our ass and take a look at our life and start fixing it. Obviously we get this feeling because we aren't in our in minds good enough for our self's. well I had this feeling a few months ago and it keeps reoccurring because I haven't done anything about it, what I want to do is out of my control I keep telling myself, but in actual fact it isn't, all I have to do is work really hard to find a job harder than I am now and save money, work harder at home and start being more considerate, there are a lot of things I need to do before my life becomes to my satisfaction , and I'm not really doing much about it, but I want to start and instead of saying I want to I am going to I need to stop thinking about it and do it , because my life isn't going to sort out itself. And neither will anyone else's.
It's like hello people wake up to your self's and get your act into gear, make your life start to happen because in time when you're like 20 , 30, or 10 years older than you are when you have this epiphany you'll be wondering what happened to your life and why didn't you change it when you had the chance instead you've sat around for years wasting your life away, and that's not something we want to do, so instead of waiting for yourself to realize your life sucks , sit back and take a look at your life and try to imagine where you might be in 10 years if you don't change your ways.


text:
Get Ready
"Come out come out where ever you are." Chuck whispered viciously, prancing up and down the dark apartment hallway. A goal on his mind, a task to succeed, Chuck chanted his sadistic words again. They were words that could make any person tremble with fear, feeling colder than the January frosty night air.
Like Millie. Hidden behind a fairly large plant, hand clamped over mouth, she waited until his footsteps echoed away. Unfortunately, they were getting closer and closer. Millie's foot slipped in nervousness and Chuck turned around to lock evil eyes with her just before she bolted away. But he was faster.
He tackled her quickly, pinning Millie to the ground. She tried to scream but his hand came down over her mouth as she struggled to get him off. With his strong hands holding her, Chuck snickered. "Finally got you. Jared will be happy now."
Chuck couldn't see but Millie's hazel eyes widened in terror from the very idea of facing Jared again. It never seemed like a possibility but now this horrendous Chuck is making all the nightmares come true. And that's not how it's supposed to happen in fairytales.
Time to come face-to-face with the truth: Millie Lane's life is probably the farther possible thing from a fairytale. For several months she's lived in despair, her emotions controlled by hatred and disgust, all directed toward Jared. Seeing his face again would confirm everything that happened in the past between them was in fact, all true.
And that's definitely the last thing Millie wanted to find out and … unfortunately, the first thing on Chuck's list of things to do. Get ready.


text:
This is a commentary on the entire episode, but I'm looking for the first 2 - 3 minutes of the episode.
Searched: Here Comes the Neighborhood South Park
This is the episode's script.
Just the wikipedia entry for this episode of South Park.
The official South Park episode guide.
http://www.southparkstudios.com/clips/153083, this clip could work, southparkstudios also offers an embed version of the clip that I'm trying to use somehow, I'm not sure I like this idea anymore, but at least I found what I was looking for.


text:
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Trip to Benidorm
Shorter driving days now we're virtually in Southern Spain, so we were in Benidorm by lunch. Saw our first Big Black bull on the side of the road. There are loads around, but that's the first we've seen this trip and Penny has them on her mind as she's just been reading about the Brandy factory and the Bulls used to advertise it (think we'll be visiting and sampling some when we get to Cadiz).
Part 5. - Lisbon to Southern Spain
... October but you would never know it! Construction was already underway over a year before Antonio Gaudi took control of the project and transforming it with his architectural and engineering style.Combining Gothic and curvilinear Art Nouveau styles. Gaudi devoted his last years to the project and at the time of his death in 1926 less than a quarter was complete. Lots of hurdles in its construction followed, notably the Spanish Civil War and then lack of finances but ...
A visit to Benidorm
... that it was very strange. I also saw a rock in the middle of the sea which reminded me of a sinking ship. We walked up to the end of the beach. At the end, there was a tiny beach that had no waves but it was guarded by a lifeguard. I didn't understand why that beach even needed a lifeguard. We walked up to point where I could see lots of tall buildings. We went ...
Beach, beach, Beach!
... When we were leaving, the sky was getting cloudy. We stopped at a large supermarket on the way back home. It was a huge supermarket. I don't know how many times more we can come to the supermarket like this in Europe. There is only a week left before going back to Australia. I really liked playing at the sandy beach today. It made me feel happy. I also really enjoyed swimming in the ocean. What a enjoyable ...
A visit to Alicante
"The sky is nice and clear..." I thought while Mum, Mitsuki and I were walking to a tram stop. Today we decided to have a day trip to Alicante. It was the closest city from where we are staying. On the way in to Alicante, we saw the beach that we tried to get to yesterday. It was very long sandy beach along the side of the tram track. There were a sand soccer pitch and a few basketball court on that beach. I saw more high rises when we were getting closer to ...


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New London - Lesbian comedian Erin Foley of California, who emceed Saturday's Pride at the Beach festival at Ocean Beach, asked her audience what it was like to be gay in New London.
The crowd hooted and applauded.
The longer answer, from the organizers at OutCT and festival-goers, was that it is getting better all the time, and not just on a summer Saturday among other members of the LGBT community. At the festival, wearing a rainbow tutu, dressing in drag or kissing a same-sex partner every 10 seconds was expected and celebrated.
More importantly, acceptance is becoming the norm in the community at large.
Drag queen Melody Lucas recently moved to the area from South Carolina with her husband, who is in the military.
"The military has been amazing," Lucas said. "We've come a ways. I think there's always room for growth and change."
Festival organizer Keri Cannon, who is a member of OutCT's board of directors, said the Coast Guard Academy recently invited the group to a discussion of gays in the military, and that OutCT also hosted a discussion about religion and homosexuality.
"We've put on quite a bit of social and educational programs in and around New London," Cannon said. "What we would like to see is for OutCT to expand, to build a nice, big, visible, positive gay and lesbian community that incorporates business as well."
It doesn't hurt that New London's mayor, Daryl Justin Finizio, is a gay man. The mayor, attending the festival with his husband, Todd Ledbetter, performed the opening ceremony. Finizio, who moved here from Rhode Island, said he and Ledbetter had one of their first dates at Ocean Beach. He said it was there that he noticed the city had "an integrated diversity," unlike other cities, where members of various communities live in their own enclaves.
"When I came to New London I noticed more interracial couples than in any other city," Finizio said. "They were young and old, so this was ingrained. There was an obvious gay couple next to a Latino couple next to an African-American couple, and everybody was being so good to each other."
Foley, the comedian, congratulated Connecticut on being "a shining example" for gay marriage, having legalized it in 2008. But not so long ago, being gay here could be very lonely.
"When I was 18, I thought I was on the only gay kid in all of Connecticut," said Chris Deveau, a 42-year-old city resident who volunteered at a sign-up booth for youth programs. He eventually found some supportive people at Yale University and in the Quaker community in West Hartford. Today, there is so much more for young gay people, Deveau said.
"It's great to see there are opportunities for youth," he said.
Curtis Goodwin, director of OutCT's new youth program, said he signed up 20 people between the ages of 13 and 19 by about 4 p.m. Goodwin, 27, of New London, said there would be separate groups for middle school children and young adults and that the focus would be on learning life skills, creating social media platforms, starting businesses and healthy living. A mother from Springfield who heard about the program dropped off five kids, he said.
Goodwin said it took a long time for him to be comfortable in his own skin, and he wants to ease the way for others.
"They're facing HIV, peer pressure and suicide," he said. "At some point, someone has to step up. My life is worth something, and I want to show them their life is worth something."
While New London's gay community turned out in large numbers, and drag queens from the city's bars were the official festival divas, Saturday's event drew visitors from distant towns and several surrounding states. The organizers estimated that about 500 people had come through as of 4 p.m., based on the number of programs they handed out.
"We go out of state a lot for this type of thing, so it's exciting for it to be here," said Samantha Sanborn of Windsor, who was relaxing on the boardwalk with her partner, "Hype" Howard. Both of the women belong to the New England Nightmare women's football team.
"It exceeded my expectations," Sanborn said of the festival. "I thought we'd be gone in half an hour."
Twitter: @KFLORIN
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: Speculation:
Luongo: The Neverending Story
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Sergei Shirokov
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Join Date: Jul 2012
Location: British Columbia
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Originally Posted by
Brad Zeimer mentioned today that Van wish list is a 3rd line center, depth d and back up...
My thoughts: With the fact it is a shortened season...
Luongo to TOR
for Bozak and MacArthur
Arnott for $1M
Vandermeer for $750 or add Franson in Lu deal and sign for $600K
Ellis for $700k
all pro-rated amounts
Daniel Sedin ($6.100m) / Henrik Sedin ($6.100m) / Alexandre Burrows ($2.000m)
David Booth ($4.250m) / Tyler Bozak ($1.500m) / Jannik Hansen ($1.350m)
Mason Raymond ($2.275m) / Jason Arnott ($1.000m) / Clarke MacArthur ($3.250m)
Maxim Lapierre ($1.000m) / Manny Malhotra ($2.500m) / Chris Higgins ($1.900m)
Ryan Kesler ($5.000m)
Kevin Bieksa ($4.600m) / Alexander Edler ($3.250m)
Dan Hamhuis ($4.500m) / Jason Garrison ($4.600m)
Keith Ballard ($4.200m) / Andrew Alberts ($1.225m)
Jim Vandermeer ($0.750m)
Cory Schneider ($4.000m)
Dan Ellis ($0.700m)
2 Pending UFA's and nothing for the cupboard. Not enough and there are a few players I would consider over Arnott.
Originally Posted by
Coming around on Shore, but would still want more along with him to make it worthwhile (definitely a 1st round pick as well). But no Shore,
No Bjugstad, No Gudbranson, No Huberdeau
, No Luongo to Florida trade.
Well those 3 definity aren't happening but we could still get something to apease us, I agree that Shore should be in if possible, although I also really want Petrovic in the deal.
Originally Posted by
Luongo to Toronto for 2 pending UFA third liners? I certainly hope Gillis doesn't blow it and do something like that. That would be an absolutely horrible return for Luongo.
Actually I think they could work out if we get more, or atleast one of them. McArthur could perhaps be a 2nd liner on our team and is a solid 2 way player (Basiclly another Higgins perhaps with more offensive skill)
McArthur, Kadri, Finn.
Sedin - Sedin - Burrows
Booth - Kesler - McArthur
Then we sign Edler, and if we have enough money at the end of the season we can try to get him signed too or else let him go and it is NBD since we get other things in the trade.
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CHAPTER 9: Potions ClassMature
Alice went to her first class for the day which, by mere coincidence, she shared with Draco, Pansy and Goyle. As they walked down the corridor to potions class with Slughorn Alice felt sott of sorry about the leaving Crabbe behind. But it was his own choice not to take potions . . . er, well, it was his own choice to fail potions last year, that is.
As they walked in Slughorn greeted them merrily, giving a completely unneccisary (and slightly uncomfortable) hug to Alice.
"Hello, Proffessor," she said. Then she'd noticed that Draco and Crabbe had already sat down and Pansy was waiting for her. Since Draco and Crabbe sat together, Alice and Pansy had to sit together. Personally, Alice would've rather sat with Cissy's son but Pansy had wanted some "girl time." The way Alice understood it was that Pansy only hung out with Crabbe, Goyle and Draco and desperatly needed another female around to keep her sanity.
"Here Alice, let's sit here," Pansy sugested.
"Okay," she replied.
Just before class had begun, Potter and the red-headed boy burst into the room.
"Oh, hello Mr. Potter, Mr. Weasley. What are you two doing here?" asked Slughorn, but then changed his mind. "Oh nevermind that, now. Well, come sit down and open your books up to 246."
"Books," said Potter as he caught his breath. "We need books."
"Oh very well then, there are some spares in the cupboard"
They ran over to the cupboard, looked at eachother and began fighting over the book. Alice happened to be trying to get into the cupboard above them to get the ingredients she and Pansy would need.
"It's just a bloody book!" she said pulling them apart and handing the new book to Weasel or whatever his name was and the old one to Potter. Okay, yes she'd done that on purpose because she hated Harry, which she supposed made her a hypocrate. Oh well, anything to get them out of the way to get her ingredients.
She walked back to the table with the tray of things they'd need.
She began cutting and adding and stirring until she'd added all the ingredients and followed most of the directions. She'd modified it a bit because she knew that two of the ingredients that it said to put together would explode when put together so she only added half of the amount the book said to. It did explode, but the explosion didn't kill anyone so it was alright. It was bubbling, why was it bubbling? Living Death isn't supposed to bubble . . . she added some wolfspring and it stopped. She tested it by dropping a leaf in it. Damn, it didn't dissolve. She sucked some venom out of her teeth and spit it into the cauldron. Rather than dissolving, the leaf caught on fire. Oh well, close enough.
When Slughorn went around to see who had won the Felix Felicis He first dropped it in Alice's since only she and Potter had managed to even finish the recepie. Draco's potion had actually GOT UP OUT OF THE POT AND CRAWLED AWAY! Alice couldn't help but laugh at that and Draco gave her a bit of a dirty look. She just smiled.
Unfortunatly Slughorn thought that the leaf bursting into flames instead of dissolving did make a difference and somehow Potter had managed to brew his perfectly so he won the liquid luck. Alice hadn't really any use for it anyway so she didn't care. She could see that Pansy was EXTREMELY jealous. Alice couldn't help but let out a bit of a giggle.


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text:
You find a playground.
You spot a fun looking playground and ask Candy if you can play there. She smiles and tells you to go have some fun, but reminds you that you don't have too much time since the other kids are going to be at the house soon. You then hop onto the round about with all the little kids. Despite your obviously bigger size, they all seem to think of you as another little kid; probably because of your diaper. Candy helps to spin the round about making it go faster. Eventually, some of the kids start to appear a little dizzy, but you're having so much fun and want to go faster. The round about is so much fun, but something happens.


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Alexander Wang puts a stylish twist on an essential basic with this cropped black cotton-knit sweater. Sharpen the louche lines and highlight the asymmetric cut with spray-on skinnies and fierce ankle boots. Shown here with Superfine pants, Kain top and an Alexander Wang bag and shoes.
- Black chunky cotton-knit cropped sweater with asymmetric cut. Alexander Wang top has extra-long sleeves and simply slips on. 100% cotton. Hand wash cold.
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Parents of kidnapped girls speak out тАк#тАОBringBackMothersDayтАм тАк#тАОBringBackOurGirlsтАм тАк#тАОBringBackOurDaughters #CNNтАм тАк
Ashie S Hirji stashed this in Africa
Parents of kidnapped girls speak out ┬аhttp://www.cnn.com/video/data/2.0/video/bestoftv/2014/05/06/exp-erin-sot-duthiers-parents-of-kidnapped-girls-speak.cnn.html┬а
Eric Holder Offers Help For Kidnapped Nigerian Girls
U.S. Attorney General Eric Holder is offering law enforcement assistance to help rescue more than 200 Nigerian schoolgirls kidnapped by the Islamist militant group Boko Haram, a senior Justice Department source told The Huffington Post on Monday.
Stashed in:
Three Abducted Girls Dead, Eighteen Others Now Sick тАУ Channel 4 NewsThe jihadis who kidnapped more than 200 Nigerian schoolgirls two weeks ago say they are тАЬwilling to considerтАЭ the release of those who have not already been trafficked abroad and sold into marriage.┬аThe girlsтАЩ abductors, who have been┬аin regular direct contact with a government intermediary, also report that three of their teenaged captives have died тАУ although they did not state how this happened. Eighteen others, they say, are now sick.┬аThe intermediary told┬аChannel 4 News┬аthat the al-Qaeda-affiliated group, Boko Haram, has threatened to kill the remaining hostages if there is any attempt by the Nigerian military to rescue the girls. Meanwhile in the capital Abuja, the Nigerian government is coming under intense public pressure to secure the girlsтАЩ release.http://omojuwa.com/2014/05/bringbackourgirls-three-abducted-girls-dead-eighteen-others-now-sick-channel-4-news/
Al Qaeda denounced Boko Haram.┬а
Adam thanks for the link, I must read ... here is the latest┬аhttp://time.com/97933/boko-haram-nigeria-ransom-obama/
You're welcome Ashie.
Some senior lawmakers are┬аfloating the idea of sending special forces┬аto help find the girls, who appeared for the first time since their kidnapping in┬аa video released by Boko Haram┬аon Monday.


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text: Posted February 18, 2008 Otter II magnum sled with otter skin lodge, square tubing, one seat, and travel cover. Bought new 2/06 and have used it approx. 9 times. Also has refectix on the ceiling for those really cold days. Set up is: 74"L x 98"D x 76"H.FIRST $395 SALE ALSO GETS: StoneLegacy rod bag (holds 2 rods and small tackle box) and a set of five S.O.S. (structure on a string). Share this post Link to post Share on other sites


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Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins
Protein and enzyme biochemistry
Methionine (Met) residues in proteins can be readily oxidized by reactive oxygen species to Met sulfoxide (MetO). MetO is a promising physiological marker of oxidative stress and its inefficient repair by MetO reductases (Msrs) has been linked to neurodegeneration and aging. Conventional methods of assaying MetO formation and reduction rely on chromatographic or mass spectrometry procedures, but the use of Met-rich proteins (MRPs) may offer a more streamlined alternative.
We carried out a computational search of completely sequenced genomes for MRPs deficient in cysteine (Cys) residues and identified several proteins containing 20% or more Met residues. We used these MRPs to examine Met oxidation and MetO reduction by in-gel shift assays and immunoblot assays with antibodies generated against various oxidized MRPs. The oxidation of Cys-free MRPs by hydrogen peroxide could be conveniently monitored by SDS-PAGE and was specific for Met, as evidenced by quantitative reduction of these proteins with Msrs in DTT- and thioredoxin-dependent assays. We found that hypochlorite was especially efficient in oxidizing MRPs. Finally, we further developed a procedure wherein antibodies made against oxidized MRPs were isolated on affinity resins containing same or other oxidized or reduced MRPs. This procedure yielded reagents specific for MetO in these proteins, but proved to be ineffective in developing antibodies with broad MetO specificity.
Our data show that MRPs provide a convenient tool for characterization of Met oxidation, MetO reduction and Msr activities, and could be used for various aspects of redox biology involving reversible Met oxidation.
Methionine (Met) is a sulfur-containing amino acid that is particularly susceptible to oxidation by reactive oxygen species (ROS). Methionine oxidation results in the formation of a mixture of two diastereomeric forms of methionine sulfoxide (MetO), which can be reduced back to Met by methionine sulfoxide reductases (Msrs) A (MsrA) and B (MsrB). These enzymes play an important role in protection of proteins against oxidative stress and have been implicated in regulation of the aging process. Insufficient repair of oxidized Met by these enzymes may alter protein structure and cause protein aggregation and/or loss of function, accelerating age-associated diseases
Conventional assays for detection of MetO and monitoring its reduction rely on HPLC and mass-spectrometry procedures
In this regard, an important approach in developing tools for MetO research is the use of Met-rich proteins (MRPs). Previously, we identified and characterized three such proteins and developed methods to monitor their Met oxidation and MetO reduction by SDS-PAGE and immunoblot assays
Computational identification of MRPs
In an initial step of the search procedure, we adopted a strategy similar to that previously used for identification of MRPs
Cloning, expression, purification and mass spectrometry analyses of MRPs
Sequences coding for MRP4 (from codon 23 to stop codon), MRP5 (from codon 21 to stop codon), and MRP6 (from codon 21 to stop codon) were PCR-amplified from the corresponding genomic DNA. MRP3 coding region starting from amino acid 22 was amplified from mRNA isolated from
Oxidation of MRPs by H2O2
Purified MRPs were subjected to controlled oxidation by various concentrations of H2O2 or other indicated oxidants, wherein oxidation was monitored by the mobility of proteins on SDS-PAGE gels. To verify that the shift in mobility was due to Met oxidation, the oxidized proteins were subjected to reduction with Msrs in the presence of DTT or thioredoxin (Trx) as described below.
Preparation of oxidized MRPs to generate antibodies specific for oxidized MRPs
Purified MRP3-MRP7 were oxidized with 10–50 mM H2O2 at room temperature for 12 h, the oxidized proteins subjected to SDS-PAGE analysis to determine the extent of oxidation, and ESI-MS analyses were carried out to measure the number of oxidized Met residues. The conditions that resulted in oxidation of all Met in proteins (these were slightly different for various MRPs) were used to prepare oxidized MRPs at large scale for immunization. Rabbit polyclonal antibodies were produced by Covance, Inc.
Enrichment of antibodies specific for oxidized MRPs
Affinity columns containing reduced or oxidized MRP were used to enrich polyclonal antibodies specific for oxidized MRPs. To prepare an MRP affinity column, 2–6 mg of a reduced or oxidized MRP (the same preparation as that used for immunization) were coupled to NHS-activated Sepharose (GE Life Science, Piscataway, NJ) according to manufacturer's instructions. The antibody purification procedure included the following two alternatives: (i) One-step enrichment. The antisera were first purified on a protein G Sepharose column (GE Life Science, Piscataway, NJ), and then the resulting IgG fraction (10–20 mg) was dialyzed against PBS and further applied to a column containing a reduced MRP at room temperature for 2 h, with shaking. The unbound fraction was collected and the Sepharose column was washed with 5 volumes of PBS, followed by elution of the bound antibodies. The unbound and wash fractions were re-purified on the column with reduced MRP twice using the same procedure. The final unbound and wash fractions were used for immunoblot analyses to examine specificity of the resulting antibodies. (ii) Two-step enrichment. The final unbound and wash fractions obtained by the procedure described above were further purified on the affinity columns containing oxidized MRPs and the bound antibodies were eluted and used for further immunoblot analyses.
Msr activity assays using MRPs
The DDT-dependent assay was carried out as following: 2–4 μg of an oxidized MRP were incubated with 5 mM DTT and 0.5-4 μM Msrs at 37°C for 2 h, and the reaction mixture was mixed with SDS-PAGE sample buffer and subjected to SDS-PAGE analysis. The Trx-dependent assay was as follows: 2–4 μg of an oxidized MRP were incubated with 6.8 μM Trx, 0.2 μM NADPH, and 0.4 μM Trx reductase at 37°C for 2 h, and the reaction was subjected to SDS-PAGE analysis.
Computational identification of MRPs
The three first-generation MRPs characterized in our previous work
Preparation of recombinant MRPs
For experimental analyses of MRPs, we selected two proteins from a previous study, including FeNos (herein designated MRP1) from
Figure 1
SDS-PAGE analysis of recombinant MRPs
SDS-PAGE analysis of recombinant MRPs. His-tagged recombinant MRPs (MRP1 to MRP7) were identified through a computational procedure, cloned, expressed in
MRP-based analyses of Met oxidation and MetO reduction by SDS-PAGE
We previously demonstrated that oxidation of Met residues in the first-generation, Cys-containing MRPs could be monitored by protein mobility shift using SDS-PAGE gels, and that MetO-containing MRPs migrated slower than the corresponding reduced proteins
Figure 2
Analyses of Met oxidation and MetO reduction in MRPs by SDS-PAGE
Analyses of Met oxidation and MetO reduction in MRPs by SDS-PAGE. (A) 60 μM MRP1 was treated with indicated concentrations of H2O2 for 7 or 12 h, and protein oxidation was monitored by the mobility shift on SDS-PAGE gels, as visualized by Coomassie Blue staining. Open triangles represent increasing treatment time (i.e., 7, 12 h). (B) 60 μM MRP5 was oxidized with indicated concentrations of H2O2 at room temperature for 12 h, and MetO formation was monitored by the mobility shift on SDS-PAGE gels, as visualized by Coomassie Blue staining. (C) Mass-spectrometry analysis of reduced MRP5 and the protein oxidized by 10 mM H2O2 at room temperature for 12 h. Theoretical and observed masses are shown.
We further examined Met oxidation by H2O2 in other MRPs by in-gel assays as well as by mass spectrometry. Met residues could be fully oxidized in MRP2, MRP4, MRP5, and MRP6 when 60 μM proteins were treated with 10 mM H2O2 at room temperature for 12 h; however, 20 mM and 50 mM H2O2 was required to fully oxidize MRP3 and MRP7, respectively. These data suggest that structural and sequence context in which Met is present may influence efficiency of its oxidation. Figure
Met residues are sensitive to two-electron oxidants
To examine sensitivity of Met residues to different oxidants, we treated 20 μM MRP5 with H2O2 or NaOCl, which are two-electron oxidants, and with CuSO4 or FeCl3, which are one-electron oxidants. Treatment of MRP5 for 1 h at 37°C oxidized approximately 50% Met residues when 10 mM H2O2 was used, and full oxidation was achieved upon treatment with 50 mM H2O2. Further increase in H2O2 levels to 100 mM did not change MRP5 mobility on SDS-PAG gels, but also did not lead to significant protein degradation (Figure
Figure 3
Oxidation of Met in MRPs by various oxidants
Oxidation of Met in MRPs by various oxidants. 20 μM MRP5 was oxidized with the indicated concentrations of (A) hydrogen peroxide (H2O2) or sodium hypochlorite (NaOCl) at 37°C for 1 h; (B) CuSO4 or FeCl3 at 37°C for 2 h, and then subjected to SDS-PAGE analysis.
Analyses of Msr activities by in-gel assays using MRPs as substrates
We further subjected oxidized MRPs to treatment with Msr enzymes (Figure
Figure 4
Msr activity assays by following MRP electrophoretic mobility shift on SDS-PAGE gels
Msr activity assays by following MRP electrophoretic mobility shift on SDS-PAGE gels. 4 μg of as-isolated (lane 1) or oxidized (lanes 2 to 12) MRP1 were incubated with Msrs and DTT or Trx as described in Materials and Methods, electrophoresed on 10% SDS-PAGE gels and stained with Coomassie Blue. Migration of reduced (R) and oxidized (O) MRP1 is shown by arrows. (A) MsrA activity assay. (B) MsrB activity assay (lanes 2 to 11) and MsrA plus MsrB activity assay (lane 12). (C) MsrBA activity assay. When used separately, MsrA and MsrB partially reduced oxidized Met residues, whereas treatment with MsrBA completely restored mobility of the reduced protein.
Antibodies specific for oxidized MRPs
To generate antibodies recognizing MetO residues, we optimized large-scale MRP oxidation by following mobility of MRPs on SDS-PAGE gels and carrying out mass spectrometry analyses. Antibodies were prepared against all oxidized MRPs, but, as expected, they were found to recognize both reduced and oxidized forms of these proteins. Therefore, we isolated antibodies on affinity columns containing conjugated reduced MRPs, and the flow-through fractions from these columns were found to be enriched for oxidized MRPs. These antibodies were designated e.g. a-MRP4-OX and a-MRP6-OX, for antibodies enriched for oxidized MRP4 and MRP6, respectively (Figure
Figure 5
Immunodetection of MRP in as-isolated and oxidized forms with antibodies enriched for the corresponding oxidized MRPs
Immunodetection of MRP in as-isolated and oxidized forms with antibodies enriched for the corresponding oxidized MRPs. (A, B) 2 μg of MRPs (as-isolated and oxidized forms) were resolved on SDS-PAGE gels, transferred onto PVDF membranes, and analyzed by Western blot assays with the antibodies against indicated oxidized MRPs. Top panel: Polyclonal antiserum (non-enriched) used to detect oxidized MRP4 (A) or MRP6 (B). Middle panel: Polyclonal antiserum against oxidized MRPs was purified on an affinity column containing reduced MRP, and the unbound fraction (designated enriched) was found to be enriched for the antibodies specific for the oxidized MRPs. Bottom panel: protein loading. "R" indicates reduced and "O" indicates oxidized MRPs. (C) 2 μg of MRP4 were oxidized with indicated concentrations of H2O2 for 12 h at room temperature and detected with non-enriched (top panel) or enriched (bottom panel) antibodies for oxidized MRP4 by Western blot analysis. (D) Western blot analysis of different amounts of MRP4 oxidized with 10 mM H2O2 for 12 h at room temperature with non-enriched and enriched antibodies for oxidized MRP4. Triangles represent increasing amounts of MRP loading (0.25, 0.5, 1, 2 and 4 μg). "MRP4-R" indicates the as-isolated MRP4 (lanes 1 to 5), and "MRP4-O" indicates the oxidized MRP4 (lanes 6 to 10).
To test if antibodies enriched for certain oxidized MRPs reacted with other oxidized MRPs, the antibodies enriched for oxidized MRP4 and MRP6 were used to probe various MRPs in both oxidized and reduced forms; the enriched a-MRP4-OX antibodies recognized oxidized MRP2, MRP5 and MRP6, but did not interact with any of the reduced MRPs (Figure
Figure 6
Antibodies enriched for the oxidized MRPs recognize other oxidized MRPs
Antibodies enriched for the oxidized MRPs recognize other oxidized MRPs. Western blot analysis of as-isolated (MRP-R; R for reduced) and oxidized (MRP-O; O for oxidized) MRPs (MRP1-MRP7) with initial antisera raised against oxidized proteins (top images in each panel) and enriched antibodies (bottom images in each panel). (A) Antibodies against oxidized MRP4. (B) Antibodies against oxidized MRP6.
Figure 7
Enrichment of antibodies for certain oxidized MRPs using affinity columns with other MRPs
Enrichment of antibodies for certain oxidized MRPs using affinity columns with other MRPs. (A) Western blot analysis of as-isolated and oxidized (10 mM H2O2, 12 h) MRP4 and MRP6 with antiserum against oxidized MRP3. "R" indicates as-isolated (i.e., reduced) protein, and "O" indicates oxidized protein. (B) Polyclonal antiserum against MRP3 was first purified on a protein G column, then fractionated on an affinity column with as-isolated MRP4, and the unbound fraction was further purified on a column with oxidized MRP4. The bound fraction from the latter column was used for detection of MRP4 oxidized under various H2O2 treatment conditions.
Finally, we tested if the resulting antibodies could recognize MetO-containing proteins in yeast extracts. The antibodies made against oxidized MRP3 and further enriched on the MRP4 column (Figure
Figure 8
Antibodies enriched for an oxidized MRP detect a candidate oxidized yeast protein
Antibodies enriched for an oxidized MRP detect a candidate oxidized yeast protein. Lysates of log-phase yeast cells (BY4741 strain), including wild-type (WT) and MsrA/MsrB double knockout (MsrA/B) strains, were treated with H2O2, and 30 μg proteins were subjected to Western blot analysis with the antibodies raised against MRP3 that were enriched for the oxidized MRP4 as described in Materials and Methods. (A) Yeast lysates were incubated with (lanes 2 and 4) or without (lanes 1 and 3) 100 mM H2O2 for 1 h at 37°C. (B) Wild-type (BY4741) lysates were incubated without (lane 1) or with different concentrations of H2O2 (lanes 2 to 5) for 1 h at 37°C. The triangles represent increasing amounts of H2O2 in the oxidation reaction (5, 10, 50, or 100 mM).
In this work, we characterized Met oxidation and MetO reduction in proteins by SDS-PAGE and immunoblot analyses using novel, second-generation MRPs as substrates. We also developed antibodies specific for oxidized MRPs that could recognize other oxidized MRPs. A key feature of new proteins identified and characterized in the current study is that they contained more than 20% Met, while lacking Cys, whose oxidation may interfere with certain redox assays involving Met oxidation. The use of Cys-free MRPs avoids a complication of variable oxidation of sulfur-containing amino acids
Whereas previous studies already showed that Met oxidation may result in a decreased mobility of proteins on SDS-PAGE gels, these studies involved a limited number of proteins, and in some cases, no changes in mobility were observed. The use of 7 MRPs, as reported in our study, demonstrates that the decreased mobility on SDS-PAGE gels is a basic property of Met oxidation. Since each of the MRPs has many Met residues, these proteins could be used to monitor Met oxidation and MetO reduction and to assess the degree of Met oxidation at any given point in the assay.
Interestingly, retardation of migration of oxidized MRPs on SDS-PAGE gels was more pronounced than that expected based on the mass difference between reduced and oxidized proteins. For example, a complete oxidation of Met residues in MRP1 increased the protein mass by 0.6 kDa; however, comparison of migration of this protein with protein standards revealed an apparent 6 kDa shift in mobility. Most Met residues in MRPs could be oxidized to MetO by treatment with 10 mM H2O2 for 12 h at room temperature, and there was an excellent correspondence between mass-spectrometry and SDS-PAGE analyses. Overall, the use of multiple MRPs allows us to state that Met oxidation that results in slower migration of these proteins on SDS-PAGE gels is a general feature of MRPs, and that it is independent of the occurrence of Cys in these proteins.
Although our data suggested that all Met residues could be oxidized by peroxide and targeted for reduction by Msrs, we observed important differences with regard to efficiency of these processes. For example, the use of H2O2 concentrations above 10 mM typically did not further change MRP migration on SDS-PAGE gels (because all Met were already oxidized at this concentration). However, calmodulin (it contained fewer Met than other MRPs examined in our study) required approximately 50 mM H2O2 for full oxidation. These data are also consistent with the observations in a previous report
The conversion of Met to MetO involves a two-electron oxidation process
Another application of MRPs is the use of these proteins as substrates in Msr activity assays. We previously found that oxidized MRPs can be reduced by Msrs in a DTT-dependent assay. In the current work, we utilized Trx-dependent reactions involving MsrA, MsrB and a fusion protein MsrBA and found that this assay is equally amenable to the analysis of MetO reduction in MRPs.
Protein state- and modification-specific antibodies, both monoclonal and polyclonal, have been a great instrument in protein analysis, and this especially applies to phosphorylation state-specific antibodies
We tested a potential application of detecting MetO residues in proteins with the antibodies against various MRPs. MRP3 antibodies enriched on the resin with the oxidized MRP4 could recognize a 62 kDa protein band in yeast lysates treated with H2O2. Since this protein was weakly detected by the same batch of antibodies in the sample not treated with H2O2, we reasoned that it is sensitive to oxidation. Previously developed antibodies against MRP3
MRPs represent a convenient tool to study Met oxidation and MetO reduction. These proteins and antibodies against them could be used in a variety of applications, several of which are highlighted in our study. MetO can be formed under physiological conditions in cells, but due to low abundance of Met residues and their random oxidation, the analyses of Met oxidation and MetO reduction have been difficult. With the second-generation MRP tools, some of the outstanding questions in Met oxidation and MetO reduction processes can be addressed.
Met: Methionine; MRP: Met-rich protein; MetO: Methionine sulfoxide; Msr: Methionine sulfoxide reductase; Cys: Cysteine; DTT: Dithiothreitol; Trx: Thioredoxin.
Competing interests
The authors declare they have no competing interests.
Authors' contributions
Designed experiments: XL, YZ, VNG. Carried out research: XL, AK, YZ, DTL, DH. Wrote paper: XL, VNG. All authors read and approved the final manuscript.
This work was supported by NIH grant AG021518.
The yeast peptide-methionine sulfoxide reductase functions as an antioxidant in vivo
Overexpression of peptide-methionine sulfoxide reductase in Saccharomyces cerevisiae and human T cells provides them with high resistance to oxidative stress
Methionine sulfoxide reductases: relevance to aging and protection against oxidative stress
Methionine oxidation and aging
Alterations in mitochondrial and cytosolic methionine sulfoxide reductase activity during cardiac ischemia and reperfusion
Methionine sulfoxide reductases: ubiquitous enzymes involved in antioxidant defense, protein regulation, and prevention of aging-associated diseases
MsrA protects cardiac myocytes against hypoxia/reoxygenation induced cell death
Overexpression of methionine sulfoxide reductases A and B2 protects MOLT-4 cells against zinc-induced oxidative stress
Impairment of methionine sulfoxide reductase during UV irradiation and photoaging
Methionine-centered redox cycle in organs of the aero-digestive tract of young and old rats
Metal binding and oxidation of amyloid-beta within isolated senile plaque cores: Raman microscopic evidence
High-affinity and cooperative binding of oxidized calmodulin by methionine sulfoxide reductase
A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation
Reduction of DABS-L-methionine-dl-sulfoxide by protein methionine sulfoxide reductase from polymorphonuclear leukocytes: stereospecificity towards the l-sulfoxide
Mass spectrometric analysis of HOCl- and free-radical-induced damage to lipids and proteins
A sensitive and specific ELISA detects methionine sulfoxide-containing apolipoprotein A-I in HDL
Detection of oxidized methionine in selected proteins, cellular extracts and blood serums by novel anti-methionine sulfoxide antibodies
Analysis of Methionine/Selenomethionine Oxidation and Methionine Sulfoxide Reductase Function Using Methionine-Rich Proteins and Antibodies against Their Oxidized Forms
Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees
Role of structural and functional elements of mouse methionine-S-sulfoxide reductase in its subcellular distribution
Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases
Functional analysis of free methionine-R-sulfoxide reductase from Saccharomyces cerevisiae
Essential role of methionine residues in calmodulin binding to Bordetella pertussis adenylate cyclase, as probed by selective oxidation and repair by the peptide methionine sulfoxide reductases
Wanted and wanting: Antibody against methionine sulfoxide
Oxidized mitochondrial protein degradation and repair in aging and oxidative stress
Cysteine function governs its conservation and degeneration and restricts its utilization on protein surfaces
Redox proteomics of protein-bound methionine oxidation
Methionine sulfoxide reductases preferentially reduce unfolded oxidized proteins and protect cells from oxidative protein unfolding
Methionine sulfoxide reductases: selenoprotein forms and roles in antioxidant protein repair in mammals
Oxidation of high density lipoproteins II. Evidence for direct reduction of lipid hydroperoxides by methionine residues of apolipoproteins AI and AII
Oxidation of high density lipoproteins I. Formation of methionine sulfoxide in apolipoproteins AI and AII is an early event that accompanies lipid peroxidation and can be enhanced by alpha-tocopherol
Comparative reactivities of various biological compounds with myeloperoxidase-hydrogen peroxide-chloride, and similarity of the oxidant to hypochlorite
Absolute rate constants for the reaction of hypochlorous acid with protein side chains and peptide bonds
Human neutrophils employ the myeloperoxidase-hydrogen peroxide-chloride system to oxidize alpha-amino acids to a family of reactive aldehydes. Mechanistic studies identifying labile intermediates along the reaction pathway
Phosphorylation state-specific antibodies: applications in investigative and diagnostic pathology


text:
At first glance, there may appear to be no similarities between lawyers and clinical psychologists. However, closer inspection reveals that they both research and analyze information to find solutions that will help people. In addition, both career options require an extensive educational commitment culminating in a juris doctorate or a Ph.D, respectively. However, the job prospects are vastly different for these two professions.
Job Outlook for Lawyers
The U.S. Bureau of Labor Statistics predicts that demand for lawyers will increase by 10 percent from 2010 to 2020, which is about as fast as the 14 percent average growth rate projected for other U.S. occupations. The general term "lawyer" includes prosecutors, defense attorneys, government counsels, corporate counsels and lawyers who specialize in such areas as legal aid, the environment, tax, intellectual property and litigation.
Job Outlook for Clinical Psychologists
BLS reports that demand for clinical, counseling and school psychologists is projected to grow by 22 percent from 2010 to 2020, which is faster than the 14 percent average. The bureau attributes the high demand for clinical and counseling psychologists to the increasing number of people with mental, emotional and behavioral problems who will seek professional help.
Best Prospects for Lawyers
Lawyers find the most job prospects in the legal services industry. The next highest employers of lawyers include local and state governments and the federal executive branch, followed by firms that manage companies and enterprises. New York hires the largest number of lawyers, followed by California, Florida, Texas and the District of Columbia.
Best Prospects for Clinical Psychologists
Clinical psychologists work in private practice, as well as working for mental health service facilities, managed healthcare organizations, schools, universities, hospitals and the government. BLS reports that elementary and secondary schools employ the most clinical, counseling and school psychologists. Health practitioner offices, individual and family services and state governments have the next-highest employment levels, followed by outpatient care centers. In terms of states, California is the largest employer, followed by New York, Pennsylvania, Texas and Illinois.
- U.S. Bureau of Labor Statistics: Lawyers
- U.S. Bureau of Labor Statistics: Psychologists
- U.S. Bureau of Labor Statistics: Occupational Employment and Wages, May 2012: Lawyers
- Society of Clinical Psychologists: About Clinical Psychologists
- U.S. Bureau of Labor Statistics: Occupational Employment and Wages, May 2012: Clinical, Counseling and School Psychologists
Photo Credits
- Stockbyte/Stockbyte/Getty Images


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Michelle Alexander Explains How Prisons Are Used To Control People Of Color
In her bestselling book The New Jim Crow: Mass Incarceration in the Age of Colorblindness, Michelle Alexander makes a poignant case about how mass incarceration is eroding the social fabric of our society without yielding any of the positive results it claims to be pursuing. In a recent interview with FRONTLINE, Alexander discussed how the war on drugs has created a system of mass incarceration and its impact on American society.
Statistics paint a bleak portrait of the decimation caused by mass incarceration in the United States, and numbers prove that the war on drugs has not only been unsuccessful but negatively impacted nearly every aspect of American life that it was meant to improve. While the crime rate in the United States in lower than the international norm, its incarceration rate ranges from 6 to 10 times higher than other countries. This insanely high rate of incarceration has led to a system that now takes freedom away from more African Americans than were enslaved in 1850 and simultaneously prevents more African American men from voting than who were disenfranchised 1870.
According to Alexander, this system of control and oppression has decimated entire communities by breaking up families and destroying any hope of economic or social sustainability. This impact is felt not just while someone is under control of the prison system but even after they are released. Many people with criminal records struggle with employment and being able to function as fully contributing member of society which creates a cycle of reincarceration that many never escape. Recidivism is also fueled by the number of people who are sent back to prison for probation and parole violations. For example, there were more people in prison for these types of violations than were in prison for all reasons in 1980 by the year 2000. This means that there are more people who are put back in prison after serving time than the total number of people even put in prison in the first place.
Mass incarceration more severely affects communities where a large percentage of the population is impacted. Communities with large concentrations of offenders generally struggle with providing job opportunities and housing because the criminal records of its members prevent them from working and being able to obtain steady housing. Alexander points out that it is this economic struggle that destroys the social fabric of a community. As people turn to drugs to numb out from the pain of living in such depraved social conditions or as a means of income, the cycle of the drug war continues to ravage black communities in a way that other communities are not impacted. Drug policy and mass incarceration must be addressed before entire generations become victim to a vicious cycle of inequality and private prison profits.


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Sasuke's reason not to leave
"Don't leave!" Sakura screamed at the top of her voice. Yeah, because hysterical shouting is just what people need in the middle of the night. "Please, Sasuke, stay here with me." She was so desperate to stop him that she would have tied him up to a tree, but she knew he would probably just turn into a goddamn log, or something, if she tried it.
Bla, bla, bla, she continued the sentimental crap. Sasuke didn't seem to be interested in any of this, so he just kept walking, hoping to get rid of that whining thing that followed him so persistently. Bad timing was something he really hated. Not that he was in such a big hurry to get to a totally perverted Orochimaru, but Konoha was already making him sick.
"Sasuke, do you remember when... ( bla, bla, bla, this is really boring, you don't need to read it ) Stay here with me. I'll do ANYTHING to make you stay!"
Anything. It's an interesting word. And usually it leads to trouble. But it certainly works. If Kakashi had begged him like this, instead of giving him a stupid lecture, Sasuke might have been persuaded. Sakura wasn't as hot as Kakashi, everyone knew that, yet Sasuke thought he could make some use of her.
He stopped and turned around very slowly, then he gave Sakura an estimating look. So she would do anything. He was tempted to tell her "go kill yourself then", but tried to think of something better.
When you spend most of your time in training, you certainly have very little time for other things. And when you finally try to steal some time for said other things and you sneak into the bushes near the training grounds, damn Kakashi pops out of nowhere just in time to tell you "Get your hand out of your pants and go back to training." And he has the audacity to tell you this while he's holding a porn book with his sticky fingers. Not to mention the fact that he must have spotted you so easily in the bushes because he happened to be there for the same purpose as you. As it was mentioned before, Sasuke really hated bad timing.
So, when a person in these circumstances is presented with the chance to come across a girl who says she would do anything, that person might start getting all kinds of funny ideas.
"Hn. Anything, huh?
"Well… yes."
"Do you know what kind of stuff happen in Kakashi sensei's books?"
Sakura nodded and blushed, confused how such a question came out of nowhere all of a sudden. Sasuke seemed relieved. He really didn't want to go into unnecessary details.
"Fine. Then take off your panties."
"Huh? What could you possibly need my underwear for? It won't fit you, it's not your colour, and…"
"Just take it off and bend over the goddamn bench!"
"Jeez! You know the pictures in those books, right? I've seen you peep over Kakashi's shoulder while he's reading."
"Oh, you mean that." Sakura couldn't believe her luck. Was he for serious?
"Yeah, that. And you'll do anything else I say. Then I might stay. Now take off your panties."
Sakura was squealing with overexcitement, surprised at his impatience. How had he managed to move so quickly from "you're annoying" to "take off your panties" she just couldn't figure out.
Sasuke smirked. "Oh, and Sakura, one more thing. If Naruto wants to do that with you, you'll let him.
"Eh? No!" Sakura was terrified. Sasuke stared at her with fake surprise. "You said 'anything', Sakura, do you want me to leave?"
She stared back at him, her face flaming red. "No! Don't leave! Oh, damn it! I'll do it."
Now that was really amusing. Sasuke smirked again. "And if I tell you to do it with someone else, no matter who, you'll do it!" he said with demanding voice. There was silence for a long time.
"Oh god, I can't believe I'm agreeing to this." Sakura sighed, cursing her obsession with him.
"Even if that someone is Lee?" Sasuke tried not to laugh.
"Damn you, Sasuke, why even Lee? Eww." Sakura lost control.
Sasuke's attempt not to laugh failed. "Because it's gonna be really disgusting and I want to see it. If you want me to stay, you'll be my slave."
"Oh... all right." Sakura hadn't expected that, but whatever.
On the next morning Izumo and Kotetsu were on their way to Tsunade's office when they found Sakura sprawled on the bench. They started shaking her. When she opened her eyes, they tried to make her tell them what happened, but she was so out of it she couldn't speak properly.
"Maybe she has a hangover," Kotetsu said. Izumo just shrugged.
"Sasuke... Sasuke..." Sakura muttered.
"What happened to Sasuke? Tsunade-sama was worried he might try to escape from Konoha."
"No, he's still here, the bastard, he just fucked the crap out of me!" Sakura whined miserably and passed out again. That's what happens when you promise "anything" to someone who hasn't had proper wanking for weeks.
"Well, that's not our business," Izumo said. "So... Let's tell everyone about it and watch how Ino and the rest will lynch Sakura."
On the next day there was a party at Sasuke's place with Naruto, Neji, Gaara, Rock Lee, Shikamaru and Kiba.
"Well, guys," Sasuke smirked like he owned the world, "First Sakura will make striptease and then all of you can fuck her."
"I'm not interested in striptease," Neji said. "With my byakugan I've seen everyone in Konoha naked, even you guys."
Their jaws dropped and they gave him horrified glares.
"Oh yeah, that's right," Neji went on, "And I know that you, Sasuke, wear pink underwear, and to be precise, right now it's Sakura's panties. And you, Shikamaru, have no underwear at all, I guess you're too lazy to put it on, and you, Lee, have a really small dick, and you, Naruto, have a tattoo "Sasuke forever" on your butt, and Kakashi sensei is really hot under the mask, Tsunade never wears a bra, and Choji..."
"That's enough! I don't wanna hear any more of this shit!" Sasuke yelled.
'Thank god he didn't say anything about Gai sensei,' Lee thought, 'I'm sure there are a lot of odd things to be seen about him... Tenten said that once she accidentally saw... well, never mind.'
Then Sakura did the striptease, which was not as amusing as they expected, and after that everyone had their turn fucking her, which turned out to be much more amusing than what they expected. The fun went for hours... and hours... and hours...
..."She's sucking Naruto and Gaara is fucking her," Sasuke said, "so there's one more free hole there, Kiba, you can join them."...
..."Hehehe, Shikamaru, I can see your cock inside her," Neji laughed, staring at them with his byakugan. "When he takes his cock out of you, Sakura, I'll give you the 64 strikes with mine"...
..."2 strikes"..."4 strikes"..."8 strikes"..."16 strikes"..."aaaaaaaa, Nejiiiiiiiiiiii"...
..."32 strikes"..."64 strikes"..."Yeah, Hyuga style, baby."...
..."Damn you, Naruto, no one wants to wait until all of your clones take their turn"...
..."Don't be so selfish, Sasuke, get off of her, it's my turn, I'll show Sakura my awesome sex taijutsu," Lee muttered. "Shut the fuck up, Lee, you're drunk, it's not your turn now."...
..."Damn you, guys, I'm not drunk. I don't wanna wait for my turn any longer. Aw, who's the pretty girl with the long brown hair and white eyes? Come here, baby, I'll show you the power of youth"... "Fuck off, Lee, you drunk idiot. I'm Neji, can't you see? Damn it, get your hands away from my butt."
Lee sneaked behind Neji's back, using the blind spot of his byakugan, and then... "Ouch! Damn you Lee... ouch, my butt... oh noooo, somebody help me, pleeease..."
'Thank god his dick is so small,' Neji thought.
Sasuke: You, writer, this wasn't supposed to be a yaoi story, right? Lee and Neji? Are you insane?
Writer: Well... um... I changed my mind. And it was you who wanted to watch disgusting stuff with Lee.
Sasuke: Well, at least don't make me get raped by Itachi again. It happens all the time in other people's stories. Man, it's so cliché!
Writer: Eh... I really love these stories and in most of them you actually enjoy it.
Sasuke: Just don't make it happen in this one, please. I'll do anything!
Writer: Anything, huh?
Well, hm, now back to the story.
All of them were having a good time, especially Lee.
"Hahaha, Lee and Neji, that was awesome," Naruto laughed. "It must be fate, Neji." He just couldn't stop laughing. "Oh, Sasuke, you're back. You look exhausted, where the hell have you been?"
"Dude, don't ask."
When all of them finally got tired and Sakura was lying in a puddle of cum on the floor, they started leaving one by one. 'Well, she's not so useless after all,' Sasuke thought.
"Hey, Sasuke," Naruto said, standing at the door. "Can I borrow her for a while and give her to Jiraiya in exchange for teaching me some new jutsu?"
"Yeah, but not this week. I already promised to give her to Kakashi sensei, and Iruka is also waiting, not to mention Ebisu. Oh damn it, I'll have to make a list."
Everyone was gone and Sasuke was alone when he heard a strange noise. He looked around and saw that Itachi was at the window, watching him.
"Itachi, how long have you been there?"
"All the time. I saw everything." Itachi smirked. "And that's the best you can do, Sasuke? Foolish little brother, you lack imagination."
"Damn you, Itachi, if you want to see imagination, go get the bitch who's writing this."
"Er... later. Now I'm here to rape you, Sasuke." Itachi's smirk got even creepier.
"No way!" Sasuke yelled, "That's not gonna happen!"
"But, Sasuke..."
Writer: Sorry, Itachi, not this time.
Itachi: But I... *tears of disappointment roll down his cheeks*
Writer: I said no!
Itachi: Pwease! *puppy eyes*
Writer: No way.
Itachi: But ...
Writer: NO!
Itachi: Aw crap.
The end
*sniff*... but... I really wanted...
Damn you, Itachi, I said THE END!
Kishimoto owns the characters... er... that's all, bye! *writer runs away before the fangirls lynch her*


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Courtyard Victorville Hesperia
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The apartment is located in a prestigious area near Via Laetana and Princesa Street.
The apartment was renovated and is in a good condition.
The apartment comprises a spacious living room and a dining room, fully equipped kitchen, 2 large bedrooms, 2 bathrooms.
- wooden windows,
- hydraulic under floor heating in ling room and dining room.
Location and nearby objects
The property is located near stores, good restaurants and coffee shops.
A Golden Visa can be obtained with the purchase of this property (residence permit for investments).


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Beatrice HainerObstetrics & Gynecology
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