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from £180 / night help Price for guests, Nights approx:
The Mill – Home 58307 Windmill
- 3 bedrooms
- 6 sleeps
- min stay varies
The Mill – Home 58307
- Windmill
- 3 bedrooms
- sleeps 6
- min stay varies
Top Review
Windmill / 3 bedrooms / 4 bathrooms / sleeps 6
Key Info
- Nearest beach 0 km
- Child friendly
- Car not necessary
- No pets allowed
- Private garden
Description from owner
PLEASE ONLY USE THE ONLINE BOOKING SITE HERE FOR STAYS OF 7 NIGHTS or 3 NIGHT WEEKENDS or 4 NIGHT MIDWEEK STAYS. FOR ALL OTHER DURATIONS PLEASE CONTACT US OR VISIT braunstonmill.couk. LATE AVAILABILITY PRICING ALSO AVAILABLE at braunstonmill.couk We OFFER A DISCOUNT OF £100 IF JUST TWO STAY AT THE MILL SUBJECT TO TERMS. THIS IS NOT AVAILABLE VIA THE BOOKING SYSTEM ON HERE.
The Mill at Braunston in Warwickshire is a detached contemporary luxury retreat which will sleep 6 in comfort in 3 double bedrooms.
The Windmill is 6 floors of luxurious living ideal for special occasions or spending time together. Four poster bed on the top floor with it's own freestanding bath!.
New for 2014 bathroom with fitted 12 jet whirlpool freestanding bath with shower including multi-jet body jets and huge rain shower.
A welcome bottle of wine along with bread, milk, butter, selection of teas and coffee's plus all you should need to make your stay special is provided.
PLEASE ONLY USE THE ONLINE BOOKING SITE HERE FOR STAYS OF 7 NIGHTS or 3 NIGHT WEEKENDS or 4 NIGHT MIDWEEK STAYS. FOR ALL OTHER DURATIONS PLEASE CONTACT US OR VISIT braunstonmill.couk. LATE AVAILABILITY PRICING ALSO AVAILABLE at braunstonmill.couk We OFFER A DISCOUNT OF £100 IF JUST TWO STAY AT THE MILL SUBJECT TO TERMS. THIS IS NOT AVAILABLE VIA THE BOOKING SYSTEM ON HERE.
The Mill at Braunston in Warwickshire is a detached contemporary luxury retreat which will sleep 6 in comfort in 3 double bedrooms.
The Windmill is 6 floors of luxurious living ideal for special occasions or spending time together. Four poster bed on the top floor with it's own freestanding bath!.
New for 2014 bathroom with fitted 12 jet whirlpool freestanding bath with shower including multi-jet body jets and huge rain shower.
A welcome bottle of wine along with bread, milk, butter, selection of teas and coffee's plus all you should need to make your stay special is provided.
For your entertainment we supply free WiFi and with Apple TV free streaming movies and internet radio.
Private Garden - A totally enclosed private garden to the side of The Mill contains outdoor seating the private 6 seater hot tub and a gas barbeque.
Perfect for a romantic break or for small groups looking for a special break away.
Well located for Heart of England & Shakespeare Country.
You can check short break availability and prices in realtime and secure your chosen stay online at braunstonmillco.uk
Welcome to Braunston Windmill.
6 floors, 2100 sq ft of comfort and style.
Come and try High Rise Living Country Style!
THE MILL Recently featured by The Guardian and BBC Good Homes as a recommended place to stay and featured in a new BBC series presented by Nick Knowles. Chosen by BBC CountryFile as one of there top 10 places to stay in the February 2013 issue.
In the Heart of England stands Braunston Mill. Situated in the historic village of Braunston famed for the canals. Braunston village is the point where the Grand Union and Oxford Canals meet. The Mill must be the most photographed building in the area.
An 18th century building with 21st century comforts.makes The Mill a detached contemporary retreat which will sleep 6 in comfort in 3 double bedrooms. Free WiFI.
Check our availability and book online at braunstonmillco.uk with the assurance you have secured your stay directly with the owner
The Windmill is 6 floors of luxurious living comprising of:
Ground floor Main entrance hall with area set aside as quiet area to read your favourite book. A W/C and utility area are on this floor equipped with auto washing machine.
1st floor This floor is dedicated to a state of the art kitchen comprising of: A stainless steel American fridge freezer, a cooking centre with separate oven, combi microwave oven and a separate steam oven. The huge centre unit contains a 60cm induction hob with extractor. The Porsche designed sink station also houses a full size dishwasher. Also in the kitchen is a large 6 seat table. From here there is also a door which leads via an external stairway down to the private secluded garden.
2nd floor This floor is the huge 21' 4" lounge. This has 2 very large 3 seat red leather gorgeous contemporary sofas plus a single chair and two fantastic matching swivel chairs! Here you can snuggle up and watch your favourite DVD or Satellite programme on the 50" Pioneer Plasma TV. We have also installed APPLE TV so you can watch hundreds of films free of charge. Or listen to music on the Bose hifi system.
3rd floor On this floor you will find our new for 2014 fantastic bathroom which is equipped with an amazing freestanding bath with an overhead shower and what a shower. The shower end has a huge overhead rain shower, a multifunction shower on a riser rail and 6 body jets. The bath is also fitted with a multi point whirlpool system. A Italian designed wc and round washbasin completes the room. Across the hall is the first double bedroom with our hand made fantastic 6'3" ROUND BED with an on-suite shower bathroom. There is a LCD Freeview TV.
4th floor On this floor there is a landing with door to balcony with commanding views across the Warwickshire countryside. Of this landing is a King size double bedroom with views towards marina and Royal Leamington Spa. The bedroom is fitted with a contemporary ceramic wash basin and has a LCD Freeview TV. This floor also has a separate W/C.
5th Floor The top of the world room!!!. This room consists of two levels. Separated by a glass and stainless steel balcony. On the lower level is a new freestanding double ended bath and a ceramic and chrome washbasin. Up a small flight of stairs to the next level is the bedroom area with a 5ft 4 poster bed. There is a LCD TV with freeview. The two windows on this level give views across the Warwickshire and Northamptonshire country side for miles.
Private Garden A totally enclosed private garden to the side of The Mill contains outdoor seating and gas barbecue ready for your use. Also is a covered private 6 seater hot tub ready for use at a maintained temperature of 38 degrees. This is available for your sole use for your stay with a small charge of £10 per use up to a maximum charge per stay of £30 to cover the cost of running.
Also Included We also provide a welcome bottle of wine along with bread, milk, butter, selection of teas and coffee's. This is not just for your first drink as most owners provide but enough for your whole stay and more! You will also find fresh orange, eggs and biscuits waiting for you. In the bathrooms we provide shampoo's, conditioners, toothpaste, shower gels and bath foams. In the bedrooms we provide both hair driers and straighteners. In fact you will find all you need so just bring a change of clothes and a tootbrush! Perfect for a romantic break or for small groups or families looking for a special break away.
Further details
Please see our full terms and conditions. You can also see all of our real time availability as well as book on line on our website where we offer special pricing.
Further details indoors
The property is furnished to a very high standard through out. The special extra touches we include also make your stay more special. These include shower and bath gels, hair shampoo's and conditioners along with the most comprehensive range of essentials for your complete stay. Past visitors describe it as if they have come into our home for the time they were there, they have this was our home!
Free WiFi at The Mill
Unlike other Windmill conversions all bedrooms are separate and you do not have to pass through any to reach the other rooms.
Due to the stairs the Windmill may not be suitable for the infirm or very small children.
Please note we do not except single sex parties. We are however pink friendly.
All terms and conditions can be seen in detail at braunstonmill.couk
Further details outdoors
In the private rear garden is situated a 6 seater hot tub which is available for your private use solely. There is parking for 2 (two) cars in front of The Mill with further parking available on Church Road.
Location description from owner
The England region
At the Heart of England sits Braunston the Heart of the British Waterways. An historic village sitting on the borders of Warwickshire and Northamptonshire. This makes staying here the ideal base to explore both the historic areas such as Stratford upon Avon, Warwick and Royal Leamington Spa and the HiTech of Silverstone.
A must to visit is the Marina, extensively restored with new warehouse style accommodation. All historical equipment and buildings have been beautifully repainted. This is now indeed one of the most impressive inland Marinas in England.
Although mentioned in the Doomsday book of 1086, Braunston remained just a rural farming community, the Manor being a major working farm house with stables, stockyard and outbuilding, until the late 1700's.
All changed with the construction of the canal, the main transport network of that time. Braunston became the main central point of this canal system. Important at last! Although commercial canal traffic finished in the 1960's Braunston is now very busy with leisure boats, accompanying tourists and related businesses.
A canal side walk will take you past Chandlers, boat builders/fitters, souvenir shops and the Old Pump House with its enormous chimney. This Pump House in times past was used for restocking the top canal ponds with water form the bottom pond Reservoirs now used as moorings for the numerous narrow boats stationed here.
Easy drive to Warwick with it's Castle, Stratford upon Avon, Silverstone and The NEC makes Braunston the ideal location.
More Less
- Great for children of all ages
- No pets allowed
Bed & bathroom
- 3 Double Beds
- 1 Family bathroom, 2 En suites, 1 Toilet Only
- Wi-Fi available
- Jacuzzi or hot tub
- Private garden
- Internet access
- Central heating
- Cooker
- Fridge
- Freezer
- Microwave
- Toaster
- Kettle
- Dishwasher
- Washing machine
- Clothes dryer
- Iron
- Satellite TV
- DVD player
- CD player
- Hair dryer
- Linen provided
- Towels provided
More Less
- Parking
- Not suitable for wheelchair users
- Check in time:16:00, Check out time:10:00
- If you have any questions about check-in or check-out times, please contact the owner/manager.
- Payment
- To be covered by Payment Protection this rental needs to be paid for online through Holiday Lettings using your credit/debit card or PayPal. Never pay for your holiday rental by wire transfer (such as Western Union or Moneygram) as this type of payment is untraceable.
- Smoking
- No smoking at this property
- Cancellation policy
View Policy
About the owner
Sandie A.
- Response rate:
- 95%
- Calendar updated::
- 25 May 2017
- Years listed:
- 9
- Based in:
- United Kingdom
- Overall rating:
Languages spoken: English
This Windmill has 3 bedrooms, 4 bathrooms and sleeps 6. It's been listed on Holiday Lettings since 06 May 2008. Located in Warwickshire, it has 33 reviews with an overall rating of 4.5. The average weekly rate varies from £995 to £1360.
The Owner has a response rate of 95% and the property's calendar was last updated on 25 May 2017.
Map and how to get there
Guest reviews
– based on 33 reviews
Very Good
33 reviews
- Excellent
- 27
- Very Good
- 2
- Average
- 0
- Poor
- 1
- Terrible
- 3
"stunning weekend getaway"
This is one of the most well equipped and dressed self catering properties we have stayed at. We are frequent mature travellers looking for unusual places to stay that meet our exacting standards. The mill exceeded on all levels, quality finish and attention to detail throughout kicked off with a very warm welcome. The uniqueness of the building was captivating with magnificent views at all levels. We visited Warwick and Stratford on Avon with ease and were within walking distant of a range of pubs and restaurants .We also met very nice friendly people who rented the cottage next door ! The six of us would thoroughly recommend a visit to the Mill and the area.
Owner's reply: This is such a wonderful review. We try very hard to meet the expectations of our guests and are pleased to have met yours. We really do hope you will visit us again in the future.
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Apartment in the centre of moraira with 2 bedrooms, 1 bathroom, living/dining room, kitchen fully equipped and balcony. it has 70 m² build, air-conditioning and it is sold fully furnished. fantastic opportunity!
We can help you to get a mortgage on favourable terms. A residence permit can be obtained with the purchase of this property. Please ask our team for more information.

subset: commoncrawl


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Tax system APPLICABLE TO PETROLEUM Operations
It is clear from the wording of this letter that this"fixed post-tax relief to the shareholders of ADM enue competitiveness of their oil operations in Abu Dhabi.
independent of the calculation of Abu Dhabi quite and should therefore, affect the basis of taxation. This special treatment w later extended to ADPC and the"fixed margin" gradually in until it reached its present amount of s1.00 per barrel.
Fixing the Price of oil for Tax or Other Purposes The old-style concession agreements stipulated a payment of royalty charges on each ton of oil produced, regardless of its sal price or the profit realized from it, indicating that governments were not really interested in the price at which the oil was sold.
Then, in 1950-1951, certain oil producing countries in the Middle East(Saudi Arabia, Iraq and Kuwait) adopted the profit sharing principle(Abu Dhabi adopted the same principle in 1966) According to this principle, the host government's share moved ed royalty per unit of production or export to 50 percent of the net company profit based on posted prices. When the financial arrangement was adopted, the host countries became directly affected by the posted price(which was a tax reference used for the calculation of the companies' profits and did n always reflect market realities).
In that period, until the 1970s, the posted prices of and companies crude oil were by the alone without any consultation with the host countries. February 1959, the oil companies decided, without Middle he governments concerned, to the price of Eastern oil by cut about 18 cents per barrel. In f the spite o companies action they went on 960 by an average of about 9 recognized Continue reading


text: Cool!IMO the only hang up to all of this is the 100Mbit ethernet on the RPi. Since this is the "I/O bus" between all of the nodes I would choose a different board. I would use a BananaPi instead which has 1Gbit ether.If you don't have high I/O load it shouldn't matter which you use :)View Instructable »


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Chapter 8Mature
"Well, at least we didn't get knocked out this time," Jessica said. "That part gets old fast."
"They did take our blood, though," David said. "So they'll be able to tell us our blood types, I guess."
The stars had moved a little. The party was raging fully now; the shouting, laughing and engine sounds had magnified tenfold and were now all Tesla Squad could hear. The boxes in between them and the party proper provided meagre sound-proofing, if any. They couldn't hear whispers, only normal talking volume or higher.
Jarred wasn't paying attention to their discussion. He had more important matters to focus on, like burning through his ropes. All it took was an almost nonexistent spark, closer to a static jolt than his normal lethal bolt of lightning, to burn through the rope. It took concentration to control it so, but it wasn't a hard task, nor was he at risk of burning himself—the benefits of a secondary, but no less important, power. But he had to concentrate to direct the spark.
He could smell burning rope, the scent like cheap tobacco and almost exactly what he imagined it would be; could feel the scorching heat close to his hands. Finally, the restriction on his arms let go and his hands were free. He held them out in front of him.
"Did you just burn through your ropes?" David asked.
"Yep," he replied.
"Okay, me next!"
"What about ladies first?" Jessica asked.
"I thought that only applied to lady-like damsels and such," David countered.
"Oooh," she said. "Touché."
Jarred burnt through David's ropes and then worked on Jessica's. As he was working on Modok's bonds they heard a bandit coming closer, muttering about checking the prisoners.
"Get ready to jump the bandit," he commanded, leaning in close to whisper. David and Jessica hid their hands and ropes behind themselves. Jarred wasn't going to risk burning the rest of Modok's rope with the bandit present. He was only halfway through the most important part of the knot, but the smoke that continuing would cause would just attract attention.
The bandit produced the keys and unlocked the cage. Modok twitched, clenched his considerably-sized hands into considerably-sized fists. He was shaking.
He snapped the ropes, which miraculously didn't give Jarred whiplash—his reflexes just that fast—and sprang up. Modok lunged at the startled bandit and smashed both fists against his head.
It took a total of two seconds.
"Well," said Jarred. "That was easy."
"Now we just have to sneak past the partying bandit horde," David said. "Maybe we should lock this guy in here?"
"And steal us some phat bandit wheels," Jessica added.
"Should be a walking cake, or whatever that phrase was," Jarred stated.
Jarred led the way. He carefully pushed the door open, though it was doubtful anyone would be paying attention due to the partying—he hoped—nor would anyone hear one little gate opening amongst the much-louder anarchistic revelry taking place.
Still, he breathed a large sigh of relief when they found their weapons and got to some bandit vehicles apparently undetected.
"Jess, you're up," he said, indicating the nearest van, a maxi taxi. "You can hotwire a van, right?"
"Yeah," she replied, "but why do we need a van?"
Modok spoke. "Because I turn to stone in daylight. I need to be inside something."
"Ah. And that would be bad because we wouldn't get paid."
"Exactly," Jarred replied.
"One stolen—er, liberated—van coming right up."
Jessica moved to the right side, picked the lock on the driver's door and disappeared into the foot space. After some tinkering a spark ignited and the engine roared to life.
Loud enough to draw attention.
"The prisoners!" someone shouted.
"Move it!" Jarred ordered.
Before the door had even finished sliding shut Jessica embraced her lead-footed nature and the van sped away from the amphitheatre, tearing up the yellowed lawn as it ventured out of the parkland, onto the car-strewn Roma Street.
The chase was on as six bandit vehicles came roaring after them: two quad bikes, three sedans and a van, each covered in various spikes, bones, and graffiti.
Jessica drove crazy. She tore down Roma street heading east to avoid the major debris on the direct path. The road turned into Albert street, then it was a right turn onto Turbot.
She accelerated then braked hard coming into the turn onto George Street. That road was a winding path between abandoned cars, the wheels crushing all manner of paper and plastic litter underneath as the van ploughed ahead. Adelaide Street was a bad idea—another gang of bandits were known to live there somewhere—so she sped on to Elizabeth Street just as the bandits began firing assault rifles at the van.
Braking hard she turned left and almost ran into an overturned bus, narrowly squeezing between it and the nearest car. The left mirror went flying.
"I know this is a chase, but please don't crash us," Jarred called out.
"Can't make any promises," Jessica replied. Another hail of assault rifle fire tore at their back, this time connecting with the window. Jarred fired back, spraying bullets at the pursuing bandits. He hit one of the two quad bikes, sending it swerving into a lane off the right side, but there were still five to go.
David also fired back, but a shotgun was almost useless at ranges much greater than in-your-face. They reached the spot where they'd encountered Modok a month and a half ago. The car pile-up was still there. Jessica turned up Albert Street and headed to Queen Street over the bricks and coke bottles. They narrowly avoided some emo kids near Hungry Jacks as they sped past amidst another hail of bullets on Queen Street proper.
"They're still on us!" Jarred called out, ducking below the shattered back window.
"You have an assault rifle!" Jessica replied. "Assault them!"
Jarred emptied the clip into the pursuing bandits, taking out one who then careened into another, the both of them flying into a throng of emos and cutting them up on their vehicle spikes.
The ammo spent, he dropped the gun and clenched his fist. Electricity crackled around it, and with a shout he unleashed that gathered power at the bandit in his direct line of fire. The resulting lightning bolt tore into the bandit sedan's bonnet and set it on fire, followed by an explosion as the fuel and oil ignited in one great fireball. The car landed on an angle and crashed to the ground, the glass fracturing and the tyres blowing out. It was spectacular.
"Score!" Jarred yelled. Then ducked as more bullets battered the back door. He felt dizzy, and his eyes and ears hurt. All this, the cost of using his power.
"How are we doing?" Jessica asked.
"Down to three!"
"We're almost out of fuel!"
The remaining pursuers closed in, and Jarred ducked to reload as David unloaded buckshot into their faces.
The bandits backed off at the end of the street. Jessica veered right.
Right onto Adelaide.
Right into another group of bandits.


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Anglo-Saxon Women and the Church: Sharing a Common Fate
€ 90,49
Lieferbar innert 2 Wochen
Januar 1999
BeschreibungThis study of literature by clerics who were writing to, for, or aboutAnglo-Saxon women in the 8th and early 9th centuries suggests thatthe position of women had already declined sharply before the Conquest a claim at variance with the traditional scholarly view. Stephanie Hollis argues that Pope Gregory's letter to Augustine and Theodore's Penitentialimplicitly convey the early church's view of women as subordinate to men, and maintains that much early church writing reflects conceptions of womanhood that had hardened into established commonplace by the later middle ages. To support her argument the author examines the indigenous position of women prior to the conversion of the Anglo-Saxons to Christianity, and considers reasons for the early church's concessions in respect of women. Emblematic of developments in the conversion period, the establishment and eventual suppression of abbess-ruled double monasteries forms a special focus of this study. STEPHANIE HOLLIS is Senior Lecturer in Early English, Universityof Auckland, New Zealand.
InhaltsverzeichnisThe conversionary dynamic - more laws for times like these; "some special irregularities in marriage" - "Theodore's Penitnential" and the case of Saint Aethelthryth; Aldhelm's "De Virginitate" - Soldiers of Christ and Brides of the Lamb; confessors and spiritual mentors - hagiographic ideals and the Boniface circle; the advice of women and Eddius's "Life of Wilfrid"; a beautiful friendship ruined - Bede's revisionist writing of Aeffled in the "Life Cuthbert'; queen conversters and the conversion of the queen - Bede's "Ecclesiastical History" and the royal marriage; rewriting female lives - Hild of Whitby and monastic women in Bede's "Ecclesiastical History"; Rudolph of Fulda's "Life of Leobba" - an elegy for the double monsatery.
PressestimmenCareful, intelligent study...questions the widely received generalization that positive Anglo-Saxon attitudes to women succumbed abruptly in the wake of, and as a consequence of, the Norman conquest. She traces the seeds of change back to Augustine's arrival in England... a rich and dense book. YEAR'S WORK IN ENGLISH STUDIESEssential work for attitudes towards women in the church in the early middle ages and its key texts are interpreted in the light of a broad reading of many others. Its strengths lie in the realms of ideas. BARBARA YORKE, SOUTHERN HISTORYHer perceptive reading of the sources leads her to challenge widely-held assumptions (and) to present many persuasive conclusions. CHURCH HISTORY (US)
Untertitel: Sprache: Englisch.
Verlag: BOYDELL & BREWER INC
Erscheinungsdatum: Januar 1999
Seitenanzahl: 336 Seiten
Format: gebunden


text:
Twitter, Inc.
About me
|Twitter, Inc.
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|May 30, 2011
|Number of add-ons developed
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|Rated 4 out of 5 stars
Add-ons I've created
Twitter Address Bar Search
Mozilla Firefox with Twitter instantly connects you with what's happening in the world. Search people and topics on Twitter using @ and # from the address bar.
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text:
Im in Marks kitchen listening to the Whiskysharts practice and i keep smiling to myself. There so much talent in one room room. These song are so fun and so inspiring. I feel more like myself every day I practice moderation.
So I walked into Dandelion cafe to meet a friend for a piano interlude in the middle of the day. As I strolled into the room I felt this really great presences. She took up the whole room while at the same time allowing room for everyone else.I've never experienced that before I was floored by the ease we all went in and out of conversation back and forth through music banging out chords and melodies manifesting fields of endless creativity. I thank today for all its given me.
I've finally begun to sober up its day 6 of no alcohol, no substances and no going out. I was reminded the other day during a bad trip that I have been so detached from myself for so long. That same sentiment was mirrored by a Psyche I saw 4 days ago in Cassadaga FL. One day at a time one step at a time out of the abyss.


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City Edge Brisbane Hotel
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Suites
- Laundry facilities (self serve)
- Conference facilities
- Breakfast Available
- Wireless internet connection in room (free)
- Wireless internet connection in public areas
Photos of City Edge Brisbane Hotel
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- Breakfast Available
- Wireless internet connection in room (free)
- Wireless internet connection in public areas
- Reduced mobility rooms
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- Microwave in room
- Air-Conditioning
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- Conference facilities
TripAdvisor Reviews City Edge Brisbane Hotel
Travel Blogs from Brisbane
Miles and smiles
Good morning.
Went down after breakfast to Queen St., walking along I saw a bird (Ibis) pointed it out to Rob by which time the bird had disappeared the other side of the seating area and which ever way we walked it was never to be seen again, Rob was then worried I hadn't taken all my tablets, knew it wasn't any other reason as we hadn't found a bottle shop last night.
Got the free ferry down the river and got off at maritime museum, didn't ...
15 October, 2015 (Lizard Snacks)
... felt really odd to scoop up a
handful of squirming crickets. They stuck to you like little pieces
of Velcro with antennae. Despite that, it was really easy to flick
them off one at a time to count the diet portions. The cute
shinglebacks, too slow, had to be tong-fed or have the crickets
squished on a rock for them.
Lunch was filled with me adding Daniel
to the camping site and Binna Burra transfer. I was ...
3 September, 2015 (Bird Play)
... happy to watch various creatures
for large chunks of time. It was a GLORIOUS afternoon, too. There was
bright, lazy sun streaming through swaying leaves and branches. The
wind was a perfect touch, keeping the atmosphere fresh and fluid.
We watched the mothers and their joeys
for a while. One mum was sleeping with her nearly bald joey poking
out of her pouch. I was delighted to finally see a koala pouch. It
was bizarre to see her pot belly bulge and wiggle with ...
Final entry
... because we didn't have much left in the tank. A walk across the river to the south bank, a pub ( of course ) and dinner at a Spanish style restaurant. Peter's daughter Anna and her partner Lexi joined us and were kind enough to order the appropriate dishes and entertain us by singing the American and Australian national anthems. I am writing this in the Brisbane airport waiting to board a flight to Sydney, then Dallas, then Chicago. ...
"Is this seriously where we're staying?!"
So waking up the next morning, with nothing packed due to last nights spontaneous binge we had a few hours to get it looking ship shape for the next lucky owners. Deciding to do it after we woke up, we thought we'd stay at the beach so we had a nice view whilst packing. This was a bad idea. We opened the van door and started throwing everything outside, which is apparently an invitation to around a thousand mosquitos to let themselves in. Trying to clean ...


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Genetic diversity assessment of sesame core collection in China by phenotype and molecular markers and extraction of a mini-core collection
Plant population genetics
Sesame (
Results from a genetic diversity assessment of sesame CC in China were significantly inconsistent at the phenotypic and molecular levels. A Mantel test revealed the insignificant correlation between phenotype and molecular marker information (
This study provides a comprehensive characterization of the phenotypic and molecular genetic diversities of the sesame CC in China. A MC was extracted using both phenotypic and molecular data. Low MD% and VD%, and large VR% and CR% suggested that the MC provides a good representation of the genetic diversity of the original CC. The MC was more genetically diverse with higher diversity indices and a higher PIC value than the CC. A MC may aid in reasonably and efficiently selecting materials for sesame breeding and for genotypic biological studies, and may also be used as a population for association mapping in sesame.
Sesame (
Abundant plant germplasm resources provide a broad genetic foundation for plant breeding and genetic research. However, large germplasm resources are also difficult to preserve, evaluate, and use
To further reduce the duplication of some accessions in a CC, a 'mini-core collection' (MC) can serve as a small, representative subset of the CC. MC have been developed and evaluated for chickpea
India, China, and Korea are the world's leading countries for sesame germplasm collection and preservation, as well as research on sesame CC establishment. Bisht et al.
This study examined the genetic diversity of accessions from the sesame CC in China. The objectives of this investigation were to comprehensively explore the characteristics of genetic diversity at both a phenotypic level and a molecular level, and then to provide a theoretical foundation for effectively protecting and utilizing sesame genetic resources. Furthermore, a MC was extracted using an advanced maximization strategy based on both phenotypic and molecular data with the aim of promoting reasonable and efficient applications of sesame accessions in breeding and genotypic biological studies.
Polymorphism detected by molecular markers
A total of 36 sequence-related amplified polymorphism (SRAP) primer combinations and ten simple sequence repeat (SSR) primer pairs were employed randomly to screen polymorphism between 12 accessions (typical accessions from each group). Of these, 11 SRAP primer combinations and 3 SSR primer pairs amplified abundant, clear, and repeatable fragments. They were then employed to evaluate the genotypic diversity of 453 accessions in the CC. In total, 175 amplified fragments were detected and 126 of them were polymorphic, with a polymorphism rate of 72%. The number of fragments detected by each primer ranged from 2 (GBssr-sa-173) to 26 (Me07Em06), with an average of 12.5. The number of polymorphic fragments detected by each primer ranged from 2 (GBssr-sa-173) to 17 (Me08Em05), with an average of 9.
Genetic diversity analysis of the CC
The Euclidean genetic distance (GD) was calculated based on data for standardized values of 14 phenotype traits. The Jaccard genetic similarity coefficient (GS) was evaluated based on data for SRAP and EST-SSR markers for each of the nine groups in the sesame CC (Table
The pairwise GS between all 453 of the accessions in the nine groups ranged from 0.4000 to 0.9892, with an average of 0.7060. The average GS (0.7865) was the highest for group III, followed by that for group VIII (0.7486). The lowest average GS was found for group VII (0.6857), indicating that genetic relationships were the closest between accessions for group III, and the second closest for group VIII. Accessions in group VII had the most distant genetic relationship, as evaluated by molecular data.
The most widely used biodiversity indices, the Shannon-Weaver diversity index (I) and the Nei genetic diversity index (h), were calculated for the nine groups based on phenotypic and molecular data (Figure
Figure 1
Histogram of genetic diversity indices based on phenotypic and molecular data for nine groups in the core collection (CC).
Histogram of genetic diversity indices based on phenotypic and molecular data for nine groups in the core collection (CC).
Principal coordinate (PCO) analysis of the CC
Results of the principal coordinate (PCO) analysis based on phenotypic and molecular data are shown in scatter plots of the first two principal components, respectively (Figures
Figure 2
Scatter plot of principal coordinates analysis for the 453 accessions in the CC.
Scatter plot of principal coordinates analysis for the 453 accessions in the CC.
Scatter plot based on (a) phenotypic data and (b) molecular data. Accessions from groups I, II, III, IV, V, VI, VII, VIII, and IX are marked with ♦, ⋄, ▴, △, ★, ☆, ▾, ⊚, and ▪, respectively.
MC extraction and comparison with CC
On the basis of data of 14 phenotypic traits and 126 polymorphic markers, a heuristic search applying an advanced maximization strategy identified a MC of 184 accessions from the 453 accessions in the CC, with frequencies of 40.62% for the CC and 4.33% for the BC. Table
Phylogenetic analysis of the CC (Additional file
Additional file 1: Figure S1
UPGMA dendrogram of the 453 accessions in the sesame CC based on molecular data. The 184 accessions in the MC are indicated by red rhombuses.
Click here for file
Figure 3
UPGMA dendrogram of the 184 accessions in the sesame MC based on molecular data.
UPGMA dendrogram of the 184 accessions in the sesame MC based on molecular data.
The mean difference percentage (MD, %), coincidence rate of range (CR, %), variance difference percentage (VD, %), and variable rate of coefficient of variance (VR, %) are used to comparably evaluate the properties of MC with CC. Over all the 14 phenotypic traits, the MD% between the CC and MC ranged from 0.64% to 2.84% in the nine groups (Table
The Shannon-Weaver diversity index and the Nei genetic diversity index of accessions from each of the nine groups in MC were calculated based on phenotypic and molecular data and compared with CC (Table
Figure 4
Genetic diversity indices of accessions in the MC and CC among the nine groups based on phenotypic and molecular data.
Genetic diversity indices of accessions in the MC and CC among the nine groups based on phenotypic and molecular data. 'Sh.W.' and 'Nei' indicate the Shannon-Weaver diversity index and Nei genetic diversity index, respectively.
The polymorphism information content (PIC) value of each group in the MC ranged between 0.1668 and 0.2289, with an average of 0.2037, whereas PIC ranged from 0.1482 to 0.2111 in the CC, with an average of 0.1818. Results of pairwise
Validation of the sampling strategy for extracting the MC
To validate the sampling strategy (the advanced maximization strategy) used for extracting the MC, an alternative strategy of random sampling was also applied, and a second MC composed of 184 accessions was established and investigated. Using phenotypic data, the Shannon-Weaver diversity index of each group from the second MC ranged from 0.7749 to 0.9784, with an average of 0.9020, and the Nei genetic diversity index ranged from 0.4652 to 0.5767, with an average of 0.5303. When based on molecular data, the Shannon-Weaver diversity index ranged from 0.2498 to 0.3609, with an average of 0.3201, and the Nei genetic diversity index ranged from 0.1610 to 0.2334, with an average of 0.2054. Results of a pairwise
Significance of the phenotypic and molecular genetic diversities of the sesame CC
The sesame CC in China is one of only three sesame CC in the world and differs from the other two collections
Genetic diversity assessments of phenotypic and molecular data were significantly inconsistent
This study assessed genetic diversity in the nine groups of sesame CC in China both at the phenotypic and molecular levels, but the results were inconsistent. Evaluated based on phenotypic data, the Euclidean genetic distance indicated that genetic relationships were the closest between accessions in group IX and the most distant in group VIII. The Shannon-Weaver and Nei genetic diversity indices indicated that accessions in group III were genetically more diverse than in other groups, while group I displayed the least genetic diversity. Evaluated using molecular data, the Jaccard genetic similarity coefficient and genetic diversity indices indicated that the genetic relationships were nearest between accessions in group III, followed by accessions in group VIII. Accessions in group VII had the most distant genetic relationship. Furthermore, the phenotypic-based cluster did not correspond with the molecular-based cluster; the correlation coefficient (
Why were genetic diversity indices of the CC much higher when evaluated using phenotypic data?
Both the Shannon-Weaver diversity index and the Nei genetic diversity index were much higher (I = 0.9537, h = 0.5490) when calculated using phenotypic data in the CC than when using molecular data (I = 0.3467, h = 0.2218). The sesame CC used in this study was extracted from the BC based on phenotypic traits data. Molecular data were not referred to because of the lack of molecular marker techniques; therefore genetic diversity was comparatively higher when examined on a phenotypic level. Genetic diversity on a molecular level was not considered in the establishing the CC. The high genotypic coefficient of similarity and the small phenotypic coefficient of distance between some accessions indicated that there were duplicates or near duplicates included within the CC. Therefore, in this study, extraction of a MC from the CC was performed based on both phenotypic and molecular data, using a diversity maximization strategy. Some genetically similar accessions were removed from the CC by further reduction; thus the genetic diversity indices evaluated by molecular data in the MC (I = 0.3861, h =0.2519) were significantly higher than in the CC, and the genetic diversity indices evaluated by phenotypic data (I = 0.9677, h =0.5530) were also enhanced.
Sampling strategy for the MC extraction and representation
Using a heuristic algorithm, Kim et al.
In this study, PowerCore was used to extract a sesame MC containing 184 accessions from the CC and the resulting collection was compared to that obtained by random sampling. The advantage of the advanced maximization strategy of PowerCore in capturing genetic diversity and in validating MC extraction was illustrated. An MC with low MD% and VD% and large VR% and CR% can be considered to provide a good representation of the genetic diversity in the initial CC
Limitations of this study and future development of the sesame MC
This study established a MC, but continued research on sesame germplasm is necessary. More accessions are being added to the BC, and with these accessions must also be updated in the CC and MC in a dynamic manner. The sesame MC presented here will be useful for efficiently selecting accessions with maximum diversity for sesame breeding, for selecting parents to generate mapping populations, or for further evaluation and restructuring of the CC. Furthermore, the MC, with its smaller number of accessions than the CC, can aid in comprehensive investigations of important traits and molecular markers in sesame. The combination of phenotype and molecular marker data can be used directly for association mapping and for developing key molecular markers associated with important traits.
This study presented a comprehensive characterization of the phenotypic and molecular genetic diversities of sesame CC in China. We extracted an MC containing 184 accessions from the CC based on both of phenotypic and molecular data. Low MD% and VD% and large VR% and CR% suggested that the MC provided a good representation of the genetic diversity of the original CC; it was more genetically diverse with higher diversity indices and a higher PIC value than the CC. The development of a MC may aid in reasonably and efficiently selecting materials for sesame breeding and for genotypic biological studies, and may also be used as a population for association mapping in sesame.
Materials and methods
Plant materials
All of the 453 accessions from the sesame germplasm CC in China were used in this study. Among them, 404 indigenous landraces were from seven agro-ecological zones (29 provinces) in China, which were divided according to climatic and geographic characteristics and the planting system. There were also 18 released cultivars from China and 31 exotic accessions from 15 other countries around the world. The accessions were divided into nine groups (with group codes I to IX), in which the first seven groups covered the seven agro-ecological zones in China
Phenotype data mining and analysis
The following 14 genetically stable and important agronomical traits were investigated: growth period, plant type, number of locules, flower number per nod, stem hairiness, flower color, seed color, capsule dehiscence, tolerance of water-logging, resistance to stem spot wilt, resistance to wilt, 1000-seed weight, oil content, and protein content. Values of these 14 traits recorded in a database
DNA extraction, PCR amplification and electrophoresis
The total genomic DNA of 453 accessions was prepared from young healthy leaves according to the cetyltrimethylammonium bromide (CTAB) method
Molecular marker data mining and analysis
All of the major DNA fragments were recorded as either 1 or 0 representing the presence or absence of the band, respectively. The pairwise genetic similarity coefficient (GS) was calculated using Jaccard coefficient
Extracting the MC from the CC
On the basis of the phenotype and molecular marker data, a MC was extracted from the CC using PowerCore software
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
YZ designed the study, carried out the molecular marker studies, performed the statistical analysis, and drafted the manuscript. XZ conceived of the study, participated in its design and coordination, provided the phenotypic traits data, and helped to draft the manuscript. ZC conducted the molecular marker studies. LW participated in the statistical analysis and helped to draft the manuscript. WW participated in the statistical analysis. DL participated in the molecular marker studies. All authors read and approved the final manuscript.
This work was supported by the Project of National Plant Germplasm Resources Protection (no. NB2012-2130135), the Project of youth science and technology morning plan in Wuhan (no. 201150431067), the Project of National Science and Technology Infrastructure Construction (no. 2005DKA21001-20) and the National Basic Research Program of China (973 Program) (no. 2011CB109304-2).
The second ten years
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Development of a groundnut core collection using taxonomical, geographical and morphological descriptors
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Establishment of a Core Collection for the Chinese annual wild soybean (Glycine Soja)
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Chinese Cornus officinalis: genetic resources, genetic diversity and core collection.
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Construction of nested genetic core collections to optimize the exploitation of natural diversity in Vitis vinifera L. subsp. sativa
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A mini core subset for capturing diversity and promoting utilization of chickpea genetic resources in crop improvement
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Development and evaluation of a mini core collection for the U.S. peanut germplasm collection
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Genetic assessment of a mini–core subset developed from the USDA rice Genebank
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Variability for Drought Resistance Related Traits in the Mini Core Collection of Peanut
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Population structure and marker–trait association analysis of the US peanut (Arachis hypogaea L.) mini-core collection
New sources of resistance to Fusarium wilt and sterility mosaic disease in a mini-core collection of pigeonpea germplasm
Diversity in Indian sesame collection and stratification of germplasm accessions in different diversity groups
Selection of a core collection of Korean sesame germplasm by a stepwise clustering method
Establishment of sesame germplasm core collection in China
Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops
PowerCore: a program applying the advanced M strategy with a heuristic search for establishing core sets
Characterizing safflower germplasm with AFLP molecular markers
How closely correlated are molecular and qualitative measures of genetic variation? A meta-analysis
Analysis of genetic diversity and population structure of rice cultivars from Korea, China and Japan using SSR markers
MSTRAT: an algorithm for building germplasm core collections by maximizing allelic or phenotypic richness
Population genetic analysis of co-dominant and dominant markers and quantitative traits
A rapid DNA isolation procedure for small quantities of fresh leaf tissue
Sequence-related amplified polymorphism (SRAP) a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica
Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.)
Characteristics and analysis of simple sequence repeats in the cotton genome based on a linkage map constructed from a BC1 population between Gossypium hirsutum and G. barbadense
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Nouvelles recherché sur la distribution florale
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MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0
PowerMarker: integrated analysis environment for genetic marker data


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Series: Chapman & Hall/CRC Monographs on Statistics & Applied Probability Volume: 62
359 pages, Figs, tabs
Nonlinear measurement data arise in a wide variety of biological and biomedical applications, such as longitudinal clinical trials, studies of drug kinetics and growth, and the analysis of assay and laboratory data. Nonlinear Models for Repeated Measurement Data provides the first unified development of methods and models for data of this type, with a detailed treatment of inference for the nonlinear mixed effects and its extensions. A particular strength of the book is the inclusion of several detailed case studies from the areas of population pharmacokinetics and pharmacodynamics, immunoassay and bioassay development and the analysis of growth curves.
Gives a very well-written account of the field over the past few decades, focussing mainly on US work and including much of the author's own, plus a glimpse of the future. It fairly reflects that literature over the years in dwelling at length on certain compuational methods for maximum likelihood estimation. There is aleaning toward biopharmaceutical applications, this being a field in which the authors are acknowledged authorities. There are no exercises, but enough detail of the methodology is given, together with helpful guidance on available software, to enable the keen novice to try his hand. Lastly, I hope I'll be forgiven for just adding a couple of citations to those given in the book: a slightly different perspective on nonlinear regression models for repeated measures is described briefly in Crowder and Hand (1990, Section 9.4) and at greater length in Hand and Crowder (1995, chapter 8). --M. J. Crowder, Biometrics, September 1997
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All prices include VAT. *Free delivery area varies by product range, see individual product pages for details. †Restrictions apply, see product lead-time and post code checker for availabilityAll sizes quoted are approximate. Products where shown painted have been decorated for photography purposes only.


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BRYAN SEELY SEATTLE, WA Bryan Seely in Seattle, Washington on Twitter: @bryanthemapsguy @maptivists Hypocrite, liar and compulsive scammer in Seattle Washington
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Genre: Drama, History
Antonio Banderas
Golshifteh Farahani
Rupert Everett
Hugh Hudson
Quality: HD []
Release: Apr 01, 2016
IMDb: 4.0
An acceptable drama starring Antonio Banderas (Desperado) and Golshifteh Farahani ("Exodus: God and Kings) and a nice support cast , as Rupert Everett , Nicholas Farrell , Maryam d'Abo , Irene Escolar , Clément Sibony , Tristán Ulloa , and Pierre Niney also star .
This film was based on a true story about an archaeologist and his daughter whose discovery of historic cave paintings sparks a firestorm . Marcelino Sanz de Sautuola Y De la Pedrueca (Antonio Banderas) , in 1868, accidentally discovered Patheolitic paintings in the Altamira cave , now famous for its unique collection of prehistoric art ,it was well known to local people , but had not been given much attention until in 1868 , when it was "discovered" by the hunter Modesto Peres , being located in Cantabria , north to Spain and next to Santillana Del Mar . Sautuola then started exploring the caves in 1875 . He did not become aware of the paintings , however, until 1879 , when his daughter Maria (as little girl played by Allegra Allen and grown-up by Irene Escolar) , nine years old at the time, incidentally noticed that the ceiling was covered by images of Bisons . Sautuola , having seen similar images engraved on Paleolithic objects displayed at the World Exposition in Paris the year before , rightly assumed that the paintings might also date from the Stone Age . He therefore engaged an archaeologist from the University of Madrid to help him in his further work . Sautuola , -along with his wife Conchita (Golshifteh Farahani)- , was the rest of his life fighting to prove that those paintings were real, trying to restore his innocence from the accusations of falsehood launched against him . But the Catholic Church (here represented by a fanatic Monseñor : Rupert Everett) and scientific society were reluctant to accept the presumed antiquity of the paintings . The French specialists , led by their guru Gabriel de Mortillet, were particularly adamant in rejecting the hypothesis of Sautuola and Piera and their findings were loudly ridiculed at the 1880 Prehistorical Congress in Lisbon. Due to the supreme artistic quality, and the exceptional state of conservation of the paintings, Sautuola was even accused of forgery. A fellow countryman maintained that the paintings had been produced by a contemporary artist, on Sautuola's orders. It was not until 1902, when several other findings of prehistoric paintings had served to render the hypothesis of the extreme antiquity of the Altamira-paintings less shocking (and forgery less likely), that the scientific society retracted their opposition to the Spaniards . As the towering French archaeologist Émile Cartailhac (Clement Sebony) was one of the leading critics ; however , subsequently , he emphatically admitted his mistake in the famous article, "Mea culpa d'un sceptique", published in the journal L'Anthropologie .
English-language film shot on location in Spain , dealing with life and happenings of a man called Marcelino Saenz De Sutuola, who realized one of the most important discoveries of the 19th century : Altamira's caves and while looking for the truth he confronts religion , faith and disbelieving scientists . As Marcelino owned the land where the Altamira cave was found , crashing against the skepticism and discredit of all experts , who claimed that the caves were false and the paintings made for the own Sautuola, in a effort to get rich . Olivia Hetreed and José Luis López-Linares wrote the screenplay about the father and daughter, whose achievement earns academic plaudits but scandalises their family for its empirical contradiction of the teachings of the Catholic Church . The film argues that the Catholic Church opposed the discovery and accuses Sanz de Sautuola of forger , but this not true . There was no official pronouncement of the Catholic Church in Spain or in the Vatican about the authenticity or falsity of the paintings of Altamira . The movie is dedicated to José Antonio Lasheras, who was director of the Museum of Altamira since 1991 until his death at February 27, 2016 in a car accident.
Fox distributed "Altamira" in Spain , where it was released April 1 and has taken in $1.3 million through May 1 , and will handle the pic in Latin America and Germany . UK's Sympathetic Ink , Kirk D'Amico's Myriad Pictures and Peter Goldwyn's Samuel Goldwyn Films will partner for the U.S. distribution of "Finding Altamira". Myriad has also confirmed first international sales on "Altamira" plus a novel virtual reality tie-in that could be used for the U.S. release . Lead producer on the picture is Spain's Morena Films . As Lucrecia Botín (Marcelino Sautuola's descendant) along with Alvaro Longoria from Spain's Morena Films produced Altamira and Andy Paterson Fox Intl. Prods. is an associate producer . Hugh Hudson (Chariots Of Fire , Greystoke , Revolution) directed a good plethora of good actors and this marks Hugh Hudson's first film he directs after a 15 year absence from feature films .


text: October 15, 2016 Leave a comment 3 Facebook Messenger tricks you'll love By Kim Komando, Komando.com 9,2919.2k Odds are, your friends and family are already hanging out on Facebook. That's why it makes perfect sense to use Facebook Messenger to communicate with them. Like most things in our digital lives, there aren't any manuals detailing how to use Messenger. Tech companies assume you'll jump on the bandwagon and figure it out.To help you go from novice to pro, here are three Facebook Messenger tricks you're probably not using. (Note: Messenger does not work the same across all devices and operating systems. If something isn't located in the menus precisely as I say, look around for a similar action. And be sure you have the latest version of the Messenger app on your iOS or Android device.) Make a group decision by taking a voteHow often have you gone back and forth in texts with a group about where to meet for dinner, which day is better for a gathering, what time or activity is best, and more?When faced with a bunch of people having to agree on a decision, start a group conversation in Messenger on your iOS or Android device. Then, take a vote.Facebook now lets you conduct polls in Messenger. Tap Polls when composing a message or tap More and select Poll. Compose the question along with possible answers and press Submit. Your poll will appear in the Messenger conversation and people in the group will be asked to vote for his or her choice. The most popular answer wins. Problem solved.When it comes time to pay for an activity, Messenger can help. Type a phrase like "You owe me $25 for the split check," and Messenger will take you to a payment page. Both parties need to have a debit card saved in Messenger. You enter this information in the More, and then Payments. Facebook is rolling out a change to this, and you may have it already. New chat bots look for certain things such as "IOU." When it is detected, a payment is requested and taken directly in the conversation feed.Next page: Send and receive secret messages that disappear after a certain time Continued on next page12Next View All BREAKING NEWS, TIPS, AND MORE LIKED WHAT YOU READ? GET MORE IN YOUR INBOX FREE. Stay up-to-date the easy way. SIGN ME UP! Leave a comment Previous Columns Facebook Live: How to broadcast like a pro Next Columns Hidden Costs of New Car 'Infotainment' Systems Related Articles 7 Facebook tips for power users 7 Facebook tips only power users know Facebook Messenger tricks you're not using yet How to send "secret" and "disappearing" Facebook messages


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Night terrors
Sage had been on the Jackdaw for a little over a week, most nights she had no trouble sleeping. But on this night her dreams were plagued with beasts, the kind the crew often jested about. Beasts from the stories' her mother told her as a child so she wouldn't stay outside past sundown.
Sage awoke, startled by a loud noise. She grabbed for her shawl to drape around her shoulders, the air was frigid. The Jackdaw was parked on a large port close to the chilled water. At night September produced condensation that settled on the windows of the air craft.
They were back in Galabria, the captain was getting his assignments from the council. He was vague and nondescript, when Sage had interrogated him about the assignment. Leaving her to guess where they would be headed next.
Sage walked into the kitchen, where she thought the noise came from. She heard nothing at first, than after a moment a soft rustling. It was coming from the supply closet. She grabbed the broom bracing herself. For all she knew that rank closet housed refugee rats. She turned the handle cautiously. Although it was hard to make out in the dark, the closet was void of anything suspicious. Crates of ammo, crates of rum and what looked like a warm pelted blanket. Sage breathed a sigh of relief. She ran her hand across the warm fur. She would make a point to complain to the captain about stashing away fine bedding when he said, he had little to spare.
Once her nerves calmed, Sage took advantage of the crews slumber. And made her way to the front of the ship. She wiped away the condensation and starred grimly out the large window of the Jackdaw's cockpit. The captain warranted her about being in the cockpit alone, but seeing as the rest of the crew was sleeping off a well earned stupor, she didn't even fathom being caught, as light as she was on her feet.


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StarGate® for fast protein expression cloning
StarGate® has been developed for the rapid systematic screen of the optimal expression (regarding expression host and/or promoter) and purification (regarding the fusion tag) system for a given gene of interest (GOI).
There are two possible cloning procedures:
Direct Transfer Cloning
The direct transfer cloning procedure is recommended for proteins with known purification conditions. With this strategy the PCR product of a certain GOI can directly be transferred within a one-step-reaction into the appropriate Acceptor Vector.
"Two-step-cloning" via pENTRY-IBA
StarGate® cloning via the pENTRY-IBA vector provides a tool for the systematic and rapid screen of the best working expression conditions for a certain GOI, where nothing is known from literature. After initial pENTRY cloning, the GOI in the resulting Donor Vector can be easily transferred by a simple one-tube reaction into a multitude of Acceptor Vectors providing different features.
Acceptor Vectors
IBA Acceptor Vectors provide different genetic surroundings for optimal protein expression and purification. These include different purification tags, promoter for a variety of host cells, signal sequences etc.
For an on overview on the entire vector portfolio please, click the Acceptor Vector Table.


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Popular property highlights
Located within 2 miles (3 km) of Kaplan University Davenport Campus, Fairfield Davenport by Marriott places you within a few miles (5 km) of Genesis Medical Center East. This 62-room hotel welcomes guests with free breakfast and conveniences like an indoor pool and free in-room WiFi. It's also close to Quad Cities Waterfront Convention Center and NorthPark Mall.
Free continental breakfast is included with your stay.
All 62 air-conditioned rooms provide sofa beds, microwaves, and refrigerators. TVs come with cable channels, and free WiFi keeps guests connected. Other amenities include free newspapers, hair dryers, and phones.
Property features
At Fairfield Davenport by Marriott, guests have access to an indoor pool, free WiFi in public areas, and a business center. You can put free parking to good use if you drive. Helpful staff can provide dry cleaning/laundry services, luggage storage, and a safe deposit box at the front desk. Additional amenities include coffee/tea in a common area, express check-out, and free newspapers.
- Free continental breakfast
- Free WiFi in rooms and public areas
- Free parking
- Indoor pool
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Fairfield Davenport by Marriott
Hotel Amenities
Hotel Amenities
The hotel offers a snack bar/deli. Guests can enjoy a complimentary breakfast. Wireless Internet access is complimentary. This hotel offers access to a business center. This Davenport hotel also offers an indoor pool, a picnic area, and coffee/tea in a common area. Complimentary self parking is available on site.
Fairfield Davenport by Marriott is a smoke-free property.
- Free newspapers in lobby
- Total number of rooms - 62
- Express check-out
- Number of floors - 3
- Elevator/lift
- Business center
- Coffee/tea in common areas
- Dry cleaning/laundry service
- Hair salon
- Free WiFi
- Picnic area
- Luggage storage
- Safe-deposit box at front desk
- Snack bar/deli
- Free self parking
- Smoke-free property
- Indoor pool
- Free breakfast
Family Friendly Amenities
- Family Hotel
- Indoor pool
- Free Wi-Fi
- Snack bar/deli
- Sofa bed
- Refrigerator
- Microwave
- Cribs/infant beds (complimentary)
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking
Room Amenities
Fairfield Davenport by Marriott offers 62 air-conditioned accommodations with complimentary newspapers and hair dryers. Premium cable television is provided. Refrigerators and microwaves are provided. Bathrooms include bathtubs.
This Davenport hotel provides complimentary wireless Internet access. Business-friendly amenities include desks and phones. Housekeeping is provided daily.
- Premium TV channels
- Air conditioning
- Free newspaper
- Phone
- Refrigerator
- Microwave
- Daily housekeeping
- Private bathroom
- Hair dryer
- Bathtub only
- Iron/ironing board
- Desk
- Television
- Sofa bed
- Cable TV service
- Free cribs/infant beds
- Free WiFi
Where to Eat
Guests are offered a complimentary continental breakfast.
Nearby Things to Do
Recreational amenities at the hotel include an indoor pool.
- Golfing nearby
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
- Accessible bathroom
- In-room accessibility
- Roll-in shower
Hotel Policies
Check-in time ends at 6 AM
Check-in time starts at 3 PM
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Free cribs (infant beds)!
- Pets allowed for an extra charge of USD 35.00 per accommodation, per stay (maximum USD 35.00 per stay)
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
Hotel Name
- Fairfield Davenport
- Fairfield Davenport Marriott
- Fairfield Davenport Marriott Hotel


text:
Chapter 67
Mr. Wells nodded, turning the picture around to look at it himself. "It does seem to be him doesn't it?" He said sounding about as surprised as Markoff was regarding the matter. "What's it been? Thirty years?"
The builder nodded.
"You live on the same street as the tower shooter don't you?" The man asked, leaning back in his chair.
"Not that one." Markoff said pointing down at the image.
"But you do have a connection to the event?"
The builder nodded once more. "Professor Grey lives across the street from me."
"Connections." Mr. Wells said. "That's why you're here."
Markoff looked at the man long and hard. "You've got to be kidding me." He said at last.
"Not at all." The man said. He extended his hand directing the builder back towards his chair.
Markoff made his way across the room and sat down. The chair gave out another shrill sound of protest against his weight.
"We've already had you on 24 hour surveillance since the incident with the stolen relic and the death of the student that you witnessed." The man continued. "This incident with your truck and the tower shooter was just too much to ignore."
"That's not the Tower Shooter who lives next to me." The builder responded pointing back towards the photograph. "I don't see how I'm connected to that one at all. I don't even know what the hell that is."
Mr. Wells glanced down. "Neither does anyone else." He muttered. A frown spread across his face. "This figure keeps popping up in our files. We don't know who or what it might be. No one has ever seen it with their own eyes and all of the images that we have of it aren't clear enough for us to tell us what exactly it may be doing or even what it is that we're dealing with."
"So what's that got to do with me?" Markoff asked again, this time more defiantly. "Why are you watching me? Why am I here?"
"There are archeologists at work in your neighborhood." The man said. "What do you know about the things that they're digging up?"
"Nothing." The builder answered. "They keep everything that they uncover in tents."
"But you did find a skull, is that correct?"
The builder nodded.
Mr. Wells folded his hands, steepleing them before his face. "What would you say if I told you that the Shady Acres anomaly matched the dimensions of the face that we are seeing in these images?"
"I'd say that you'd probably know more about that than me." Markoff said.
"What do you think?"
Markoff closed his eyes and pictured the figure in the image. He could still see its pale head sitting abnormally large on top of its sticklike body. The eyes were large and drawn into the skull. The jaw-line was small and diminutive. "Maybe." He answered.
"Connections." Mr. Wells repeated. "There are too many connections here."
"I'm not connected to squat and you know it!" The builder barked getting up from his seat and pointing down at the man in a blind rage. "You're just grasping at straws and you know it. You called me in here because the car that was used to bomb the mall once belonged to my wife before it was stolen. Maybe that's weird. Maybe that's something that you need to look at but now you're just throwing a bunch of random crap that you've somehow found out about my life against the wall to see what sticks. It's a waste of your time and a violation of my rights as a citizen. I'm not going to stand for it."
"Calm down." The man said. "We can't kill you."
"Then what can you do?" Markoff asked. He put his hands on his hips glaring at the man.
Mr. Wells gave the builder an apologetic smile. "I'm sorry." He said glancing down at his paperwork. "This is completely unseemly I know. I'm used to operating in a more clandestine capacity. These interviews are unexplored territory."
"You suck at them." Markoff agreed.
"We can't kill you." The man said again. "The best that we can do is freeze your finances."
"You can't do that." The builder argued.
"Yes, we can." Mr. Wells smiled. "I don't want to do that kind of thing. It's messy and it tends to make people unhappier than they already are. We're trying to prevent stuff like that rather than provoke it."
"Then what do you want?" Markoff seethed. He was livid at the thought of one organization having so much power over his life. He couldn't tear his eyes off the file. It was crammed so full of paperwork that it looked like a book. He wondered what information was contained within its multi-colored and uneven pages.
"I want you to sit down." The man said motioning towards the chair and using a direct and official tone. "I want you to tell me why your name keeps coming up in the things that I look at regarding the mall bombing. I don't care what you say, just make me believe it. I'm trying to help you."
"What's come up besides the skull and my wife's car?" The builder asked. "What else do you have in that file there that links me to being unhappy and evil."
"You've been letting foreigners worship strange Gods in your exotic looking shopping center." Mr. Well replied.
"Some of them are worshiping Christian Gods." Markoff shot back. "I just talked to one out in the lobby and he explained the whole thing to me."
"Then explain this to me." The man said slapping his hand against the desk. "Why is it that you busted in on one of our retired operatives during his re-identification assignment?"
"What?" The builder asked narrowing his eyes and cocking his head.
"Mr. Markoff, my name is Dan Wells." The man replied, leaning forward. "Does that name mean anything to you?"
Markoff looked at him. "That's my neighbor's name."
"Yes!" He said angrily. "It is your neighbor's name! We're all named Dan Wells."
The builder was starting to think that this was part of some elaborate joke. The questions didn't make any sense. "What the hell is going on here?" He asked
"You tell me!" The man said, slapping the table again. "All I know is that my organization had to stop killing people because of one botched raid in Jordan."
"That's not my fault." Markoff said defensively.
"No, but you walked in on the sole surviving member of that mission just as he was beginning to come out of his shell." Mr. Wells barked.
"He looks like a kid."
"Which is precisely why we can't have him working for us any longer." The man added. "Do you see my face Mr. Markoff?"
The builder regarded the Dan Wells who was sitting before him now at the desk. He was handsome and distinguished. A light dusting of grey sat at the corners of his immaculately combed hair.
"We can't afford to be doing what we do if people don't see us as adults." The man continued.
"What do you want with my neighbor?" Markoff asked slowly.
"Who?" The man asked. "Dan Wells or the tower shooter?"
Mr. Wells gave him a nod. "Fair enough."
The builder stood there waiting. He could just make out a few of the words that were printed in the file. "Debt" was one of them. "Rollercoaster" was another.
"Mr. Markoff, we don't want these things to happen." The man began giving him a heavy shrug and a sigh. "We want people to work and shop and be happy with as little distraction as possible. It's important for preserving the way things work. Without those basic things the system breaks down. Imagine how frightened people would be of the meteor if they didn't have new things to buy."
"And Dan Wells and the Tower Shooter?" The builder prodded.
"Connections." The man said again. He glanced down at the photograph. "On one hand you've got an ex-agent of ours who's main responsibility was to prevent stuff like this from happening. He was retired and is now locked away in a safe-room."
"What do you care?" Markoff asked. "You said yourself that you guys can't use him anymore because he looks like a kid."
Mr. Wells nodded. "This is true." He acknowledged. "We also can't kill him because we aren't allowed to do that sort of thing anymore."
"Why would you want to?"
The man reached into his desk and pulled out another photograph, this one in full color. It appeared to be a satellite image of the builder's cul-de-sac. Red blotches emanated out from various points on the top down view of their houses indicating where the greatest points of thermal energy were located. Markoff noticed that the sections surrounding the holes that the archeologist had dug were bright orange. A firey red one stood out at the center of Dan Wells house.
"He's in there working on a weapon." The man said. Looking at the image he shook his head sadly.
Markoff waited for him to continue.
"Let me put it this way." Mr. Wells continued. "There's a new thing under where you live. It's too much to explain right now and honestly there's no reason for you to know. Suffice it to say that we know what it is and we know that we don't want it getting out."
"And my family is living on it?" The builder asked.
"Yes." The man said seriously. "It probably can't hurt you if it's not activated but there's our agent." He tapped the photograph in the area of Dan Wells house. "He's got one of them and he's turned it on."
"One of what?" Markoff said.
"It's terrible." The man said as he grasped at the sides of his head with his hands. "It's something that ugly and primordial but still shiny and new."


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Spook Takes Charge ... Sort Of
"Where is Ryan?"
"Manh?" Spook looked up at the sound of Hermanus' voice. She was pacing around in circles, chewing her lip and trying desperately to work out what the heck was going on. Her spear-stick lay propped against a wall. Spook knew she should probably keep it close, but she was too preoccupied. Besides, it hadn't done her much good other than made Elektra Van Helsing nearly fall over laughing at the sight of her brandishing it in her face. The memory of her taunting still make Spook's face burn when she thought about it.
"I saw him attack the General," Alara put in helpfully, "but I do not know where he has gone now."
"Oh gawd." said Spook. Just another thing to worry about.
She looked across the room at Elorie and the man she presumed was her husband, but she knew Elorie would be too distraught to offer any help. The characters were watching her expectantly. Alara was fiddling with her bowstring, and Ash and Morgan were standing side by side by the door while Hermanus' eyes flickered from Spook to his author and back again. Spook almost laughed at the ridiculousness of the situation. Warriors, fairies and princes were looking to her for leadership. If the situation hadn't been so bleak, or Spook so close to a breakdown, she might have found it hilarious.
Ohmahgawd, what am I going to do? Spook thought in a panic. She was still sweaty and panting from the fight in the garden, and her limbs were quivering with the adrenaline and terror that had come from facing the very villains she had created. She could still scarcely believe what was happening; here she was, in a strange country surrounded by fictional characters and people she'd never met, armed with nothing but a kitchen knife on a stick, fighting off villains who never should have lived anywhere outside of her imagination. Elorie's husband was injured, Elorie herself distraught, Ryan missing and now it seemed it was Spook who would have to take the lead. Spook was only fifteen years old.
She sat down on the floor and started chewing her thumbnail, legs crossed and back hunched as her eyes began to blur with tears. She didn't know what to do. There were people out there who wanted to murder them all, and somehow everyone wanted Spook to fix it. She could see it in their eyes, a faint glimmer of hope that perhaps the being who had brought them into existence could produce some fantastic plan that would make lightning bolts rain down from the sky and zap the villains into a fine red mist. But she couldn't. She was far away from home, she was scared, confused and utterly at a loss as to what to do.
"Stop it." said Spook out loud. "Stop it right now! Bursting into tears is not going to help, I have to do something! We need a plan, if we have a plan it will all be okay. Right, plan, plan plan, I need a plan."
"Spook?" Alara raised her eyebrow at her author, utterly bewildered. "What are you doing?"
Spook didn't answer. Instead she got to her feet and started pacing the room again, muttering and mumbling under her breath, occasionally punctuating her sentence with a virulent Latin curse. The characters watched her, their faces switching from incredulity to anxiety to downright consternation as Spook continued her internal debate. Finally, she stopped and turned to them.
"Alara and Hermanus, you two go outside and keep watch." she said.
"Keep watch for what?" Hermanus asked.
"Fluffy bunnies and unicorns." said Spook waspishly. She was in Leader Mode now, and anyone who was foolish enough to cross her would be thoroughly chewed for their ignorance. "What do you think, dummy? If you see any of them nearby, send them packing. Preferably with an arrow or two up their backsides."
Alara nodded and towed Hermanus out of the door. Spook turned to Ash and Morgan next.
"Right, Ash, you go and look for Ryan. Keep your sword with you, and yowl if you see anything that might jump out and try and kill us."
"What about me?" Morgan asked.
"I need you to help me out in here." said Spook. She saw the agonised look in Ash's eyes and sighed, "Sorry lovebirds, but that's the way the cookie crumbles. Ash, get your shift your butt out there and start looking."
Reluctantly, Ash began to head towards the door. Spook took a deep breath and turned to Morgan.
"Right, we're going to need an ambulance. Grab the phone and call 999 -"
"What?" Morgan looked confused. "You mean, 911?"
"Yeah yeah," said Spook, somewhat distractedly. She was ticking things off on her fingers, trying to work out what they needed, "And we'll need something to pad his wound and try and stop the bleeding. Grab an old jumper -"
"Is that a sweater?" Morgan asked.
"That thing." said Spook. "Also, we might want to unblock a couple of these barricades to let the paramedics in. Could you do some sort of protection spells for us? You know, just in case anything else leaps out of the woodwork and tries to suck our brains out through our eyeballs. Hmm ... we're gonna have to move the sofa ..."
"Same difference!" Spook yowled. "Honestly, am I speaking Korean? Agh," she knuckled her forhead, "pox on trans-Atlantic language differences. Anyway, just get on the phone and say we need an ambulance. I'll go and rummage for some sort of bandage."
"Umm ... okay." Morgan nodded hesitantly and headed off to find the phone. Spook took a few more deep breaths to fight down the fear rising in her throat and scampered off to find a makeshift bandage, wriggling through the barricades Elorie had evidently erected around the house and muttering to herself as she hunted.
"This house is hereby declared a Not-So-Safe Zone, and everything in it subject to any slightly haphazard and impromptu usage we may find, including for defence, attack, protection or painful bludgeoning," Spook giggled, "by order of General Spook, and the High Command of Oh-Dear-We're-In-Trouble-Now!"
In the other room, Spook heard Morgan begin to speak into the phone, stumbling and stuttering over her words as she struggled to pluck up a sufficient amount of courage.
"H-Hello? Umm, c-could you send an ambulance please? We have a bit of a - a situation on our hands ..."


text:
Our websites
This is Stockholm
Modernity meets tranquility. Trend-setting restaurants and vibrant nightclubs next to idyllic archipelago. Welcome to a city where it is easy to breathe – and to feel your pulse.
Efva Attling's jewelry collections are distinguished by words and phrases on jewelry in simple basic designs executed in silver. In addition to rings and necklaces for both men and women, you'll find her designs for glasses and glass. The third store, which is the Efva Attling Flagship store, is located at Birger Jarlsgatan 9.
Hornsgatan 42-44
118 21


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ED Magedson – Founder
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Brent Ferris/greater ft.worth computer repair100 E.14th st. Joshua, Texas United States of America
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Anadarko Petroleum (APC) shares jumped as much as 14% Thursday afternoon after the oil and natural gas company agreed to settle environmental claims brought by the federal government and an umbrella group of plaintiffs called the Litigation Trust.
They settled for about $5.15 billion.
Plaintiffs, including the Justice Department and Environmental Protection Agency, had sought $20.8 billion.
Anadarko bought Kerr-McGee in 2006 for $16.4 billion cash, about three months after Kerr-McGee spun off its chemicals business as a company called Tronox (TROX), leaving hundreds of toxic sites that included uranium mines in the West and wood-treatment plants that used toxic creosote in Mississippi and Pennsylvania, the government said.
Tronox filed for bankruptcy in 2009 and sued Kerr-McGee over toxic site-related debt. The Justice Department and EPA, on behalf of the U.S. government, which is Tronox's largest creditor, joined the suit.
Anadarko said in a statement Thursday that, in exchange for complete release from all claims against Kerr-McGee, it agreed to pay $5.15 billion to Litigation Trust plaintiffs. The sum represents $3.98 billion in principal plus 6% interest accrued since the filing of the complaint in May 2009.
The government and Anadarko's Kerr-McGee unit also agreed not to sue each other. The government also will provide what Anadarko called "contribution protection" from third-party personal litigation claims against Kerr-McGee at more than 4,000 sites covered by the covenants.
"This settlement agreement with the Litigation Trust and the U.S. government eliminates the uncertainty this dispute has created," Anadarko CEO Al Walker said in a statement, "and the proceeds will fund the remediation and cleanup" of toxic sites and related claims.
In December, U.S. Bankruptcy Judge Allan Gropper ruled that Kerr-McGee had improperly unloaded its environmental liabilities onto Tronox before the Anadarko takeover and said Anadarko's liability would amount to $5.2 billion to $14.2 billion, plus attorney fees.
Anadarko said it has a "significant cash position" and a $5 billion credit facility available to fund the settlement payment.
The court still must approve the agreement.
Anadarko shares jumped 14% to 98 before the close in the stock market today, Thursday.
Follow James DeTar on Twitter: @IBD_JDeTar.


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Traille and Harinordoquy could not refuse the financial offers made by one of the biggestspending clubs in France and they will be part of a powerful Biarritz team that face London Wasps, the reigning Cup champions, at Wycombe on Sunday.
While Wasps have to operate within a £1.9million salary cap imposed on all Zurich Premiership clubs in England, teams like Biarritz spend up to £7m a year to try to become kings of Europe.
The fact Wasps recorded a European Cup and English championship double last season on limited finances and with a small first-team squad makes their achievement even more remarkable. Now they have to defend the Cup with the same handicap system in place.
This financial imbalance shows no sign of changing, raising the spectre of top English players heading across the Channel to make their fortune. Wasps captain Lawrence Dallaglio turned down the opportunity to join either Biarritz or Stade Francais before pledging his future to the club, which makes last season's double success even sweeter.
England wing Dan Luger made the move and is now playing for Perpignan in a squad containing South African, New Zealand, Romanian, Australian, Argentinian and Irish players. This League of Nations exists at every major French club and only the smaller Japanese club competition can offer a financial alternative.
In his role as managing director of player management company Inside Rugby, former England coach Dick Best has considerable experience of placing players with clubs all over Europe and he believes Wasps' achievement last season was amazing given their salary cap.
He said: "The major clubs in France like Toulouse, Biarritz, Perpignan, Castres and Stade Francais have huge playing budgets - up to £7m a year. The budgets are so big because a player going to France can expect to receive a good wage on top of free accommodation and a car, which doesn't happen here. Those benefits in kind will be taxed in Britain but it isn't the case in France and that makes the package even more attractive to a player.
"Another major factor for the French clubs are the municipal stadia that they receive, while our clubs in England have to pay to improve their facilities.
"It gives the French clubs a huge advantage and when you realise the kind of figures we are talking about, it makes what Wasps achieved with their salary cap and small squad really fantastic.
"They beat Toulouse, the biggest club in France, in the Heineken Cup final.
"Toulouse have the highest wage bill because they boast the biggest squad. With the city being the third highest in terms of living expense in France, you can see why the wages have to be so good to attract players. You also have to add to the equation in France the salaries of the coaches and the travelling around the country for matches. France is attractive for players, even though they probably play more games than in England. If you want to earn more money and play in just a dozen or so matches, then head for Japan."
Damian Hopley, chief executive of the Professional Rugby Players' Association, believes there is growing support for an end to the salary cap in England, although he admits the artificial handicap has helped bring financial stability and improved playing standards across the Zurich Premiership.
However, Hopley is adamant the salary cap would not stand up to a legal challenge as it is restrictive on the players. He said: "What Wasps did last season was very special, operating under a salary cap against French clubs who do not have that restriction on their spending. I am certain something is going to have to give in England in the near future.
"There is a growing feeling in England that the cap should be challenged, although the necessary majority is not there and I can understand that situation.
"The municipal funded stadia make a huge difference to the French clubs who can boast 20,000-capacity arenas they didn't have to pay to build. You need the government to help our game with funding for stadia improvements.
"If things don't change you have to ask: How long can we hang on to our top players before they are lured to France?"
- More about:
- Argentina
- Biarritz
- Lawrence Dallaglio
- Romania


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by, 28-Jul-2016 at 21:22 (123 Views)
The wild raspberries are finally ripe in the driveway, or ripening everyday anyway. I've picked about a quart each day this week and now my arms and legs officially look like I was in a fight with a cat... And lost. So worth it though.


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"Home is the place where, when you have to go there, they have to take you in." – Robert Frost
More often than not, our home and nationality are beyond our choosing. We enter this world as tiny, crying bundles of life, and while we can fight and work our hardest to change our destinies, much of our futures are already laid out for us. Where we're born, the background we're born into, who our parents are, our socioeconomic statuses, our legal rights: it all comes down to luck.
According to the website ifitweremyhome.com, "the lottery of birth is responsible for much of who we are. If you were not born in the country you were, what would your life be like? Would you be the same person?"
Some of us may never dream of having our life be any different than precisely the way it is, but for thousands if not millions of people around the planet, each week is filled with daydreams of where else they'd rather go or who else they'd rather be.
Are you happy with your life? These statistics may change how you feel.