text:
Mwembe Resort
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this resort rated in the past?
Photos of Mwembe Resort
Travelers also recommend:
TripAdvisor Reviews Mwembe Resort Malindi
Travel Blogs from Malindi
Moping around Malindi
From The Voyager, we took a matatu to the Nyali City Mall, grand in name and quite impressive in fact. A matatu is the local descriptor of a minivan driven without regard for any other objects, stationary or moving, human or inanimate, and is best described in our own culture as a rapid means of transport to hell. I offered 100 Kenya shillings ($1.20) to the pimp who acts as the matatu conductor; he immediately said that was not enough. I flicked out ...
Snorkling with Italians
... beachboys because then you will pay more.
- A snorkeling trip you can get for 2.400 Ksh including 1.300 Ksh park fees.
- To go to the Gede ruins from Watamu just get on the matatutu for 40 Ksh and walk a bit to the site.
- For souvenirs go to some small shops between Tiki guesthouse and the road to the beach. It is close to the Exim bank. I promised those nice ladies to promote them so here it ...
Nathalie&Cie plays Jackass
... br> Nathalie&Cie shot up in the air and claimed her victory! (Not bad for a sport has-been!)
That did get me the acclamation from security :-)
Rest of the afternoon was spent nibbling while watching Kenyan dancers ... I do like their music very rhythmic!
Anyway...need to do a wild exit again... Time to get down to the restaurant!
And food is FOOD!
Catch you later ...whouuuushhhhh...(already at the door)
Glass towers
... which juts out beyond the room into the great nothing outside the tower building itself. Encased in rather delicate ironwork so one would find it hard to roll out of bed and catapult oneself to the ground, I agree, but I don't think it would prove a restful night. There were two German girls staying with Nani, and they were sleeping in the tower in this flying bed and in true spirit found it wonderful. Not being great with heights myself, I would be happy to ...
A massage like no other I have ever had!
... a massage by one to the trainers, Zoe. I had been given a hint or two that it was extremely painful and even big boys apparently cry. Mmmmm? I'm almost ashamed to say what actually happened. Suffice to say, there wasn't a monkey on the continent that didn't hear me! Zoe did prepare me by saying that I should do my breathing as if I was in labour. I had quadruplets. Now you can imagine the pain I was in. I was breathing so heavily ...


text:
The vehicle in your driveway is head and shoulders above the rest compared to all the other vehicles these days. When your Chevrolet K10 Pickup left the factory, it was destined for a driver who wants long-lasting quality in their automobile. Anyone who owns a Chevrolet knows that when it comes to maintaining their automobile only the absolute best replacement parts will do. Chevrolet vehicle owners have come to expect a certain level of reliability when driving their K10 Pickup around town.
An important factor in fuel efficiency is the appropriate mix of air and fuel. Your Chevrolet K10 Pickup has an internal combustion engine that mixes fuel with air before it detonates, and an air filter needs to remove dust and debris before it is ignited. Without an air filter or air cleaner in your engine, your fuel would be filled with particulates from outside. This will plug up your injectors and rob your engine of fuel; leading to a lack of power produced by your engine. And unless your Chevrolet K10 Pickup air filter is replaced regularly it will fill up with dust and dirt, reduce the amount of air entering the intake, and create poor gas mileage. Clearly, replacement air filters are a necessity for everyone.
Procuring new Chevrolet parts from www.carpartsdiscount.com means you are paying the lowest prices for the most superior original and aftermarket replacement K10 Pickup parts on the internet. Buy all the car parts you will ever need from carpartsdiscount.com and realize incredible savings on all your upcoming repairs. Given all the hassles associated with performing maintenance on your car or truck, let www.carpartsdiscount.com be the number one store you go to make purchasing it easy. All the Chevrolet K10 Pickup Air Filter & Cleaner parts on this page will fit vehicles made in 1974, 1973, 1972, 1971, 1970, 1969, 1968, 1967, 1966, 1965, 1964, 1963, 1962, 1961, 1960. Choose your year below to refine your search.

subset: commoncrawl


text:
- Explore community
- Identify
- Help
Common Funnel
Observed: 15th November 2009 By: Tuisku
Added: 7th December 2009
Beautifully shaped buff coloured mushroom.
Other observations of Common Funnel (Clitocybe gibba)
I agree that it could well be
7 December 2009 - 8:31pm miked
I agree that it could well be this species although there are a couple of others that look rather like this too. Did you smell it, many of the clitocybes have interesting smells that can sometimes help with identification.
What else could it be?
8 December 2009 - 9:39am Tuisku
I did not smell it, but later read from my mushroom book about the smells! What other species could it be? I am no expert :-)
Common Funnel
8 December 2009 - 6:03pm Exobasidium
Another possibility is Lepista flaccida.


text:
Spatio-Temporal Coincidence and the Grounding Problem
- Cite this article as:
- Bennett, K. Philosophical Studies (2004) 118: 339. doi:10.1023/B:PHIL.0000026471.20355.54
- Citations
- 389 Downloads
A lot of people believe that distinct objectscan occupy precisely the same place for theentire time during which they exist. Suchpeople have to provide an answer to the`grounding problem' – they have to explain howsuch things, alike in so many ways, nonethelessmanage to fall under different sortals, or havedifferent modal properties. I argue in detailthat they cannot say that there is anything invirtue of which spatio-temporally coincidentthings have those properties. However, I alsoargue that this may not be as bad as it looks,and that there is a way to make sense of theclaim that such properties are primitive.
Copyright information
© Kluwer Academic Publishers 2004


text:
Start a Trial
Sign in
Historical Events
Family Trees
Become a Member
Become a Member
New Braunfels Herald Zeitung Newspaper Archives
September 21, 1993
- Page 1
First Name
Last Name
With All of the Words
With the Exact Phrase
Go to advanced search
Discover Your History
Issue Date:
Tuesday, September 21, 1993
Pages Available:
Previous Edition:
Sunday, September 19, 1993
Next Edition:
Wednesday, September 22, 1993
New Braunfels Herald Zeitung
Publication Name:
New Braunfels Herald Zeitung
New Braunfels, Texas
Pages Available:
Years Available:
1952 - 2013
Learn More About This Publication
2.17+ Billion Articles and Growing Everyday!
More Than 400 Years of Papers. From 1607 to Today!
Articles Covering 50 U.S.States + 22 Other Countries
Powerful, Time Saving Search Features!
Find Your Ancestors Now
Newspaper Archives
Birth Records
Census Records
Marriage Records
Death Records
Divorce Records
Military Records
View Sample Pages : New Braunfels Herald Zeitung, September 21, 1993
Page 2
Page 3
Page 4
Page 5
Page 6
Page 7
Page 8
Get Access to These Newspapers
Plus 2.17+ Billion Other Articles
NewspaperARCHIVE.com -
Used by the World's Finest Libraries and Institutions
OCR Text
New Braunfels Herald Zeitung (Newspaper) - September 21, 1993, New Braunfels, Texas TUESDAYThree Comal County volleyball teams play - P.5 50 CENTS COUNTDOWN: 448 DAYS New Braunfels Sesquicentennial March 21, 1845 March 21, 1995 New Braunfels Herald 8 Pages in one section ■ Sept. 21,1993 Serving Comal County ■ Home o! DANNY GARCIA Vol. 141, No. 217 INSIDE Obituaries........... 2 Crossword.............3 Opinion...............4 Sports................5 Classifieds.............6-8 STAMMLIS( ll Birthday wishes! The New Braunfels Herald-Zeitung extends the following birthday wishes; Danny Garcia, Larry Allen, Hattie Anthony, Juanita Sandoval, Carlos Ramirez, Justin Urias (belated), Mireya Morales (belated), Rey Castillo (belated). Bracken VFD Ladles Aux. meeting The Bracken Volunteer Fire Department Ladies Auxiliary will meet today at 9:30 a.m. at the Firehouse Community Hall. Canyon High Friends off Education meeting Canyon High School Friends of Education will meet Tuesday, Sept. 28 at 6 p.m. in the high school library. To be eligible for the scholarship drawing, members must lave joined by Nov. 1,1993. Collegiate as well as vocational scholarships will be awarded. Dr. Jerry Major, district superintendent, will give a brief talk on school taxes and facilities. AARP meeting on tap today The American Association of Retired Persons (AARP) will meet today at the Senior Citizen Center at 9:30 a.m. Refreshments will be served al 9 a.m. Members should bring items for a white elephant sale and members with September birthdays should bring cookies. New members and guests are welcome. People Against Violent Crimes meeting Thursday People Against Violent Crimes will meet this Thursday al 7:30 p.m. at the Comal County Senior Citizens Center. The public is invited to attend. The guest speaker will be Mr. Martin Allen, court administrator at the Comal County Courthouse. Items accepted at Fair tomorrow The Comal County Fair will be accepting items for exhibit in the areas of arts, crafts, photography, baking, canning, antiques, preserving, agriculture, clothing, handwork, heirlooms and horticulture on Wednesday, Sept. 22. For additional information, please consult the fair tabloid or contact Mozelle at (210)-625-1505. Special ribbons with the 100th commemorative seal will be given for winners. Elderhostsl alums invited to bo on host team Elderhostel alums are invited to be on the host team of the local Elderhostel, sponsored by the Historic Museums Association, Oct. 3-8. Call Clyde Blackman al 629-1572 to be an ambassador. The group will meet at the Sophienburg Museum, 401 West Coll, 5 p.m. today for orientation. Stxmmtisch (The New Braunfels Herald' Zeitung invites its readers to submit items to Stammtisch. According to the Sophienburg Archives and members of the German community, "Stammtisch " represents a sitting place for members of the community to gather and share the day's happenings. We invite you to share with us.) Doug Miller named finalist in the Five Outstanding Young Texans Award for 1993 From staff reports Statewide recognition was given to Doug Miller at a recent meeting of the New Braunfels Chamber of Commerce Board of Directors Miller was recognized as one of the finalists in the Five Outstanding Young Texans Award for 1993. The Outstanding Young Texans Award Program is sponsored by the Texas Junior Chamber of Commerce and Blue Cross and Blue Shield of Texas. Miller received the Outstanding Small Business Award from the National Federation of Indepen dent Businesses. U.S. Senator Phil Gramm made the nomination and said "I want to commend Doug for his willingness to invest his time, talent and resources," Chairman of the Texas Water Commission, John Hall said of Miller, "Doug Miller is an outstanding individual and is a good problem solver." After legislation was completed to settle the Edwards Aquifer issue, the Chairman of the Texas Senate Natural Resources Committee, State Senator Ken Armbrister said, "The work that Doug Miller accomplished for Central Texas and for the entire state in helping craft a solution to the com plex Edwards Aquifer issue was outstanding There is no doubt in my mind that Doug Miller is an outstanding Texan and deserves the respect of all Texans for his unselfish efforts in helping to bring this issue to a successful conclusion." Former U.S. Senator Bob Krueger said, "Doug Miller is a real asset to Texas. His leadership goes far beyond his years I truly expect Doug to be one of the future leaders of our state " Miller has served as the youngest Mayor of New Braunfels, the youngest recipient of the Besserung Award (Outstanding Citizens Award), and the youngest water negotiator among the various cities and communities affected by the Edwards Aquifer Quoting from Miller's nomination for the award "Miller has voluntarily given of his time and money to serve his community in trade associations, public affairs, natural resources activity and is currently serving on the Chamber's Board of Directors and as Drive Chairman for the United Way. His leadership has greatly benefitted the community in terms of social, economic, spiritual, and philanthropic service. Peace on the Plaza participants say they are fortunate By JENNIFER ROMP EL Staff Writer Several participants of Saturday's Peace on the Plaza said the event has caused them to realize how lucky they are and has spurred them to take action by helping with Habitat for Humanity- Peace on the Plaza was an all night event held this weekend in the New Braunfels Utilities parking lot. It was sponsored by Habitat for Humanity and several area churches. More than 70 area students participated in the event. The purpose of the event was to teach area students and passersby about the problems of the homeless and how they can help through Habitat for Humanity. Canyon High School student Matthew Flume, 17, said he had a great time at the event. "It was an experience that changed my life,'' he said. "I now have a new perspective on how the homeless live and the hardships they go through. Flume, who is president of the CHS student council, said he will sharing what he has learned with other student council members. "I hope to see what we can do to get something started in student council," he said. Canyon High School student Aimee Westfall said the event gave her the opportunity to meet a lot of new people and learn a little about what it's like to be homeless. "I don't know if I learned exactly what it's like to be homeless, but I educated myself a lot," she said. Westfall said she hopes to work with the Habitat for Humanity chapter in New Braunfels when the group starts building its first home. Samuel Clemens High School student April Harris, 15, said the event helped her appreciate what she has. "I learned not to take what I have for granted," she said. Harris said participants slept for about an bour and a half on cardboard boxes. "I wouldn't want to do that every night," she said. Some of the other activities during the event included eating a dinner of cold Beanie Weanies that was distributed in trash bags, drinking from a water hose, hearing from speakers for Habitat for Humanity and a homeless shelter in San Antonio, playing games and participating in a worship service. Psychic Fair not what most think it is By JENNIFER ROMPEL Staff Writer Visitors to Saturday's Psychic Fair at El Jardin Real ranch in New Braunfels may have found that visiting a psychic doesn't include entering a building through beaded doors and looking into crystal balls. In fact, the fair, which was sponsored by the Inner Peaoe Movement, gave area residents the opportunity to learn how to become their own psychic. Approximately five psychics and members of the Inner Peace Movement were on hand Saturday to provide psychic readings. In addition to this, a free lecture was held to teach visitors how to become their own psychic. Dwight Fehlmann, a member of the Inner Peace Movement, helped organize the fair. According to Fehlmann, I PM is based on the fact that "everyone is searching for a greater understanding of life, what their purpose is, how they can fulfill their potential. The Inner Peace Movement is an avenue for people to find these answers within themselves. "We all have a spiritual nature that has a big influence on how we perceive things and how we interact with other people," he said. Fehlmann said the Inner Peace Movement allows people to work with their inner sensitivities, build an awareness on a spiritual level and work with that to find a balance in their lives. "It's designed and organized in a way for people to make spiritual things practical," he said. Fehlmann said tapping into a psychic ability can help a person in both personal lives and business. "it helps to try to get a sense of timing of where things fit and when," he said. "Everyone is already psychic. They just don't know it. Everyone gets impressions, hunches, and inner knowings all the time," said Fehlmann. "Instead of ignoring it they can pin it down and follow through on it" The fair was held at El Jardin Real on Hueco Springs Loop, which is owned by Dr. Francisco Coll, founder of the Inner Peace Movement and the Americana Leadership College. The Inner Peace Movement was founded in the early 1960s and has grown to include approximately one-half million people. Herald Zeitung photo by JOHN HUSETH Annual Health Fair at Senior Center County nurse Shel McWilliams (left) tests the blood of Ken Pittman (right) for blood sugar, hemoglobin and Iron level during the annual Comal County Senior Center Health Fair, held Friday. NBISD meeting set tonight By ROSE MARIE EASH Staff Writer Herald Zeitung photo by KARLA WENZEL Comal County Fair royalty Shown from left are Angela Francene Lucas, Princess; Valerie Marie Morga, Queen; and Traci Lynn Tuch, Duchess. All were chosen as new title holders Sunday night at the 1993 Comal County Fair Queen's Contest, held at the Canyon High School Commons. The New Braunfels Independent School District board of trustees will meet Tuesday, Sept. 21, a 7:30 pm in the Education Center Board Room at 430 W. Mill St. The agenda includes: ■ Approval of personnel authorized access to confidential information on handicapped students. ■ Approval of Gifted/Talented Education handbook including updated grade-level and campus level curriculum descriptions ■ Approval of elementary grading and promotion policies. ■ Approval of the addition of bus services for hazardous areas and to relieve overcrowding and long waits. ■ Reports on prekindergarten and kindergarten grading standards, results of SAT testing ('92-'93) and NBISD instructional program organizationFor news, advertising or subscription information, call 625-9144 ;
About Us
Contact Us
Free Newspaper Archives
© Copyright 2017 NewspaperARCHIVE.com. All rights reserved.


text:
David O'Brien (racehorse trainer)
David O'Brien is an Irish former racehorse trainer. He was the son of highly successful trainer Vincent O'Brien. His primary successes included Assert who won the 1982 Irish and French Derbys and Secreto who won the 1984 Epsom Derby, defeating El Gran Senor trained by his father Vincent,[1] making Davis O'Brien the youngest trainer to win the race.[2] He retired from training in the late 1980s and moved to France where he is involved in wine production
- ↑ "Vincent O'Brien". The Daily Telegraph. 1 June 2009. http://www.telegraph.co.uk/news/obituaries/sport-obituaries/5422320/Vincent-OBrien.html. Retrieved 11 May 2010.
- ↑ Smith, Brian (1991). The horse in Ireland. Wolfhound Press. p. 208. ISBN 9780863273117.
|The above article was imported from Wikipedia.org on 08/24/2010 and may be a modified version of the Original Wikipedia article. See this article's history page for a list of revisions which may have occurred after import. This article is displayed here courtesy of the Wikimedia Foundation, Wikipedia, and its authors/contributors in compliance with the Wikimedia Terms of Use Agreement. This article was imported and is licensed under the terms of the Creative Commons Attribution/Share-Alike License 3.0 (CC-BY-SA). Clicking on photos, videos, or drawings in this article will display copyright and license information for that non-text media where available.


text:
17250 N. 43rd Ave #3
Glendale, AZ 85308
Here at Gentle Touch Family Dental Care, your comfort is our number one priority. We will do our utmost to put you at ease and keep you feeling relaxed and secure. Under the experienced leadership of Dr. John Oatis, our dedicated team of professionals is at your service to explain your treatment thoroughly and answer any questions you may have.
Gentle Touch Family Dental Care practices the highest infection control standards to protect our patients and ourselves. In the Glendale, AZ area, Dr. John Oatis and his staff strictly observe all Occupational Safety and Health Administration (OSHA) guidelines. All instruments are cleaned, disinfected and routinely sterilized as recommended by the American Dental Association and the Centers for Disease Control. Whenever possible, disposable, single-use items are used to ensure your safety.
If you have older dentures or think you may need dentures, you're in luck. As people age, dentures can make them look older than they really are and can cause discomfort when chewing. Glendale area dentures dentist Dr. John Oatis can replace worn out dentures with top quality custom made, state-of-the-art dentures that blend with your skin, hair and even eye color. Your new dentures will look as natural as possible, and you'll look and feel younger, too.
At Gentle Touch Family Dental Care, we offer quality, personalized care. Serving the Glendale, AZ area, we're happy to discuss the variety of treatment options available to you. If you're interested in sedation or sleep dentistry options, please schedule an appointment with John Oatis, D.D.S., and we can provide you with all the information you need.
Don't let untreated dental problems get you down. Rebuild your confidence with a beautiful new smile. From simple treatments that make a big difference, to more extensive work, unveiling the smile you were meant to have can have profound life-changing effects. Serving the Glendale, AZ area and offering cosmetic dentistry, Dr. Oatis can help you develop an affordable treatment plan you'll feel good about.
Gavin, Michael D.D.S.
6565 E Carondelet Dr # 355
Tucson, AZ, 85710-3523
Smith, John P D.D.S.
7493 N Oracle Rd # 217
Tucson, AZ, 85704-6366
Valley Dental Specialty Group
3085 W Ina Rd # 101
Tucson, AZ, 85741-2382
Yuma Dental
550 W 16th St
Yuma, AZ, 85364-4632
Maybe the reason we find an eight-year-old's gapped teeth smile so adorable is...we know it's not permanent. Soon the grown-up teeth will be poking through, clean and new. All part of the mouth's natural evolution.
It's not often that nature gives us a second chance, but when it comes to teeth it does just that. For many children it can be a wake-up call to start taking dental hygiene seriously. As the old saying goes, "God gives you two sets of teeth, but you have to buy the third."
It may be time though to change that outdated piece of wisdom to: "Teeth are like friends...ignore them and their needs and they'll go away. Be kind to them and they'll stick with you forever."
That's why regular hygiene appointments and a gum disease examination are so important. Barring injury, there's simply no reason why most of us should be missing teeth as we grow older. Call it a "10,000 smile checkup," insuring all your parts are in good working order.
Once your cosmetic dentist knows that your teeth are healthy and the gums are sound, he or she can do a little restorative dentistry to evolve your smile to an even higher level. Things like dental bonding to close the gaps…or invisible dental crowns or veneers for front teeth…even teeth bleaching for a truly dazzling and beautiful smile.
Give your cosmetic dentistry professional a call. He or she can help you see to it that your mouth does the job it was intended to for a lifetime. And look drop-dead sensational at the same time!
Gaps between your teeth may be considered by many as unattractive and make you feel self-conscious about your personal appearance. If you have spaces between your teeth, and you want to do something about them, talk to your cosmetic dentist about the latest in orthodontics and other aspects of restorative dentistry. However, before you can make a decision on which cosmetic dentistry treatment is the right one for you, it is important to know what causes spaces between teeth.
Each person is unique, and no one case presents a definitive reason why a space is present. Genetics can play a major role in how teeth form in your mouth. If your parents have spacing between their teeth, it is a good chance that you will also.
Childhood habits largely affect the position of your teeth as well. Breathing mostly through your mouth and sucking on your thumb are examples of habits that will affect the position of your teeth.
Adults can also have habits, like forcing their tongue against their upper teeth or pen biting, that can gradually shift teeth throughout life. When the teeth shift, spacing or even jaw joint pain can occur.
There are four ways to correct the spaces between your teeth. Diagnosing the reason the spaces are present will determine which of the treatments will best suit your needs.
Gaps between teeth can be filled by:
Placing braces on your teeth in order to move them into their correct position is usually the most ideal treatment. Using orthodontics to move the teeth is the most time consuming, but it is also the most beneficial. Orthodontics is used to place teeth so that your bite is in harmony with your smile.
All of the other treatments to fill the spaces, including tooth bonding, dental veneers like Lumineers, and dental crowns, although they are very good treatments, are considered esthetic and functional compromises, and should be discussed with your cosmetic dentist in order to determine which restorative dentistry treatment is best for you.
By Benjamin O. Watkins, III, DDS


text:
So you think it's easy being lean? In a society where the population is bloating out of control and obesity is normal, it would be so much easier being fat.
If I Didn't Care About Being Lean.
If I didn't care about being lean I could eat whatever I wanted, whenever I wanted. Just imagining it makes my mouth water. Cheesy pizzas, broiled burgers, Mexican delights could be eaten at all hours of the day or night. That would be incredible.
Counting calories would be a thing of the past. In fact, I would stay in denial. If you asked me how many calories and fat grams my favorite foods engulfed, I wouldn't have a clue and I would like it that way. Ignorance and food are bliss.
No reading nutrition labels for me. After all, the vocabulary needed to understand health information, such as trans fats, processed foods, cottonseed oil and net carbs is much more complex than being able to order a thing on a stick, a pocket of pleasure, or fun on a bun.
Food Labels:
Of course I wouldn't just eat out of hunger and for nutrition. No-sir-ee, I would consume out of boredom, happiness, sadness, being socially acceptable and maybe even the occasional eating contest. You've seen those on TV. They air those contests on the cable channels. It seems that it must be a sport and all sports are respectable, right? Maybe I can strive to eat the most hot dogs at one sitting this year!
The benefits would surely add up if I just gave up on fitness. Think of the money to be saved by frequent all-you-can-eat buffets, ordering fast food and pre-prepared meals in a box. I'd be rich! When I did want to splurge and spending money was not an issue, I would never struggle like I do now to find a restaurant where I could find something to eat.
The days of hunting down an acceptable size portion of nutritionally balanced fare that won't send my arteries into a future state of panic would be long gone. It would save me so much time and frustration by no longer bogging down waiters with special requests to "hold this", "put on the side that" and "cook it plain." Simple ordering right off the menu - now that's the life for me! I would probably even have money left over.
Speaking of food, I wouldn't have to spend time and energy preparing, packing and porting my own food anymore. Being fat would make my life so much easier, yes, indeed. No more measuring, planning and thinking. I can't be wasting (pun intended) my time doing those chores. My favorite TV shows are on! Why worry about trying to be lean? It's such a pain.
I could be relaxing on the sofa, kicking my feet up and pressing the remote control. Set a bag of chips and a soda pop next to me and I'd really be in heaven. I would have all the time in the world to watch all the reality shows while I really spread out my bottom by being sedentary. Oh, but you may eventually see me out of the house. Maybe I could eventually try out to be the Biggest Loser!
Fitting In:
Deciding to go fat would sure make it easier at the office. All those business lunches and dinners would be a piece of delicious cake! After all, it's a requirement to eat, drink and be merry with clients, isn't it? Here's another biggee at the office - fitting in. No, I don't mean fitting in the chair, I mean fitting in with the crowd.
At my lean present, I'm like an outcast. It's not fair! I want to be popular, too! Have you noticed that most people are more accepting of people like themselves? I'm a stick in the mud being lean. Others are constantly telling me that a little cake won't hurt me, or that it's a special occasion (and we all know that calories don't count on special occasions) or to just try some.
- Bodybuilding With Diabetes. - By Christopher Mohr
- Diabetes Detox! - By Amy Davis
- Other Diabetes Articles...
They constantly try to undermine my health conscious routines. Could my co-workers feel convicted by my healthy eating? Nah, they're just trying to help me fit in and be popular. In fact, I could win friends and influence people by bringing the daily donuts and be lovin' it while doing the lunchtime fast food runs!
I can't even complain about aches, pains and ailments with my friends and family. In my current leanness I am deprived of describing the woes of high blood pressure, type two diabetes, fatigue and joint pains. I don't get to visit my doctor as much as everyone else does. Come to think of it, they take more time off of work being sick than I do, too.
Not only would giving up being lean make me fit in with society more, but I could practically wear whatever I wanted to without being questioned. Wearing a nice outfit now can make me appear suggestive. I would blend in with everyone around me no matter what I wore if my clothes were only a few sizes bigger.
Here's another benefit of not being lean. I would be like many other moms. After birthing two children, there is no reason for me to put this much work into my body. No one expects me to be fit after two pregnancies!
Gosh, at my son's end of the year award ceremony, most parents took up one or more chairs for each cheek! That's two to three chairs a piece! I want my share of the chairs! Besides, commercials would remind me that it's not my fault. My stubborn belly fat is because of cortisol and I can just pop a pill to fix it if I were inclined to do so. What could be better?
Let's not forget the most obvious and dreaded time waster, exercise! Exercise would no longer be required! Parking in further spots from my destination and stairs would be a thing of the past. I would trade those antiquated designs for the comforts of modern technology such as elevators and escalators.
I wouldn't waste time to make time for exercising on a daily basis and I wouldn't feel guilty about it. Heck, if I did exercise, I certainly wouldn't work hard or sweat. I would read the paper, a magazine, or use it as a social event. After all, it does SOUND good to say you exercise.
I could save even more money by eliminating my current gym fees and trainer fees. So many people tell me they can't afford a trainer. It must be true because they seem to have the money to eat out, have their nails done and make all those visits to the doctor that I don't get to schedule.
They have to have money for sitting at movies, buying those big screen televisions and acquiring the latest x-boxes and video games. I could probably afford those luxuries, too, if I gave up exercise.
Role Model:
My kids would enjoy those riches as well. I could stop worrying about being an example of health to them, teaching them about nutrition, energy and movement. However, my children could still use me as a role model. They could still eat like me, look like me and learn to deal with their health issues in the future like most of their peers will.
Why shouldn't they fit in and be part of the growing trend, too? It's only right to let them do what the other kids do and be like the other kids their own age. It used to be in to be thin, but now fat is where it's at!
Training Children:
So Much Easier
Yes, it would be so much easier if I didn't uphold to staying lean. I could keep putting off until tomorrow the things that I should do today. Making excuses for taking responsibility for my health could be a top priority.
I think I could be really good at that. I probably don't get a chance to overburden the healthcare system right now like I could be. I pay dearly for health insurance premiums. I want to use it, too! Gosh, if I just kept eating more and exercising less I could eventually qualify for that really nifty, popular, trendy surgery - gastric bypass!
Come to think of it, if I weren't lean I could give up pushing myself, challenging myself, and seeing what I'm really capable of achieving. Easier is just plain easier. No pain means no pain. Most things worth getting in life are simple and pain-free, right?
In fact, it's getting much more difficult all the time to fit in and not feel like an odd ball, a freak of nature, being lean as the population of obese people swells. Just take a look around you at any public gathering. If the color of health is green, then we as a society we are becoming more magenta - the very opposite color.
The magentas have virtues regarding health that are different than the greens. We may all possess discipline, honesty and commitment, but they are manifested in vastly different ways. One way is easy and the other is not. Let's look a little closer at these virtues.
The lean greens are disciplined with their diet, exercise and preventative health. The massive magentas are disciplined in their eating traditions and keeping their doctor visits.
I would be much more popular if I did not have to be honest with people when they ask me what it takes to be lean. I would enjoy telling people what they wanted to hear instead of what they needed to hear. To be lean, it also takes being honest to yourself regarding what it is that you are eating.
We can't forget commitment. Commitment is a wonderful thing. I can't deny that the plump public is still very committed. The world abounds with people who while not at a healthy weight are nevertheless very committed to certain foods and drinks they will just not give up.
They can be very dedicated to beer, fats, restaurants and excuses. In fact, some are quite obstinate in their refusal to remove or even limit those foods in their diet. Ironically, those same people are the first to hop on the trendy, fad diet band wagons in their virtuous quest for commitment. C'est la vie.
If only I could allow myself to fully relax - in my stretchy pants, of course - I would just let go of the whole prospect.
It's Not Easy Being Lean
No, it's not that easy being lean.
Perhaps that childhood song of a wise, familiar, green friend is beginning to acquire a new and everprecious meaning for me:
It's not that easy being lean;
Having to spend each day eating to fit in your jeans.
When I think it could be easier being plump, or pudgy, or fat -
Or something much more maintainable like that.
It's not easy being lean.
It seems you don't fit in with so many
other contemporary things.
And people tend to pass you over 'cause you're
Not eating all-you-can-eat buffets and donuts and beer-
Or heapfuls of pie.
But lean's a beautiful thing.
And lean can be cool and healthy-like.
And lean can be big like with muscles, or strong
Like an athlete, or fit as can be.
When lean is what you want to be
It could make you wonder why, but why wonder why?
Wonder, I am lean, and I'm healthy,
It's beautiful!
And I think it's what I want to be.


text:
Selective Mutism: My story so far
Selective Mutism is when someone really, really wants to speak but can't. This is something that I suffer from everyday. It's very rare, but it definitely needs more awareness.
Hello, my name is Christina. I have straight red hair, brown eyes, and I'm quite tall - though not as tall as my brother. And he's a year younger than me! Anyway, I'm fifteen years old and I have this horrible thing called Selective Mutism.
I suffer from this every single day, and I've had it for years. Do you know someone that dosen't talk to you at all? Do you think that he/she is shy? Well, Selective Mutism is much worse than shyness. It stops you from talking to your friends, if you manage to get any friends. It stops you from asking the teacher for help when you're stuck. It even stops you from talking to your family.
So, more about me : I love music. The music I like? Miley Cyrus, Demi Lovato, Vanessa Hudgens, Hilary Duff, Westlife, Take That. They're all awesome. I love reading - my favourite books are the Harry Potter series! I love writing. My favourite subject at school is English. I also enjoy Art. I love being creative and using my imagination! It may seem boring to you, I don't know, but I also like History - I think it's so cool to be able to understand the history of what's happened in the world.
At school I've found that my love of English conquers all my other subjects. So what do I want to become when I'm older? My biggest ambition is to become a writer. And I think that my rolemodel for that is J.K Rowling. I look up to her as the best writer ever. I hope that when I'm older I can write a book about Selective Mutism, explaining how it really is and how it really feels. My life ambition is also to write an autobiography, and I know that Selective Mutism will be part of it.
So I guess I should say what Selective Mutism really is, because to me it really does matter because it's kind of controlling my life. So, to begin with, what I want to say is that I know that I call it "Selective" Mutism, but that's only because that's what it has been called by, let's face it, people that don't have a clue. See really "I don't choose" not to talk. That's the main key point. Does it make sense? You know, when I was diagnosed with it I was like "Oh. Really? Is there something wrong with me?" I didn't even realize that there was something "different" about me. Know what I mean? I thought that I was okay. Normal. But oh how wrong I was.
Even though I'm only fifteen I feel like I've had so many experiences already, and I'm still going through them. And there's still so much of my life left to live. But it feels like the world has stopped for me, and my entire world revolves around my Selective Mutism. So, to make myself clear, here's what I have : Selective Mutism. Hope I cleared that one up.


text:
It only takes a minute to get started. Fill out our simple sign up form and login to the site.
This step is noncompulsory but will greatly raise your chances of getting interest from other over 40s members on the site. Write about yourself, your hobbies and upload your best photograph.
Use our website's cutting-edge search to locate over 40s members who suit your needs. Or just browse through listings and locate people that pique your interest.
Identified someone who interests you? Send them a few words or just a wink and see if anything happens. With a little luck you'll be on your way to finding the love of your life.
It'll only take 30 seconds


text:
Zimmerman Homes For Sale
Zimmerman is also home to "Wild West Days" a Memorial Day celebration that hosts a charity 5k, softball tournament, carnival, parade, and many other fun activities in Lion's Park – just one of the beautiful parks in the city. Take a look at our Zimmerman homes for sale, and let us help you find your new home!
Median Home Price: $180,540.00
The City of Zimmerman offers a wide range of recreational facilities and activities for residents of all ages to enjoy. From ice rinks to disc golf to ball fields and more, there's always a fun and exciting way to get active. The city also provides programs including youth and adult sports leagues, gymnastics, and more.
Zimmerman is filled with a plethora of shops and stores to fulfill every need. From grocery retailers and service shops to florists and antique stores, residents can easily find everyday essentials as well as specialty items. Whether you need sporting equipment or new furniture, you'll find what you're searching for in Zimmerman.
Whether your family is filled with picky eaters or adventurous foodies, you'll find something for everyone to enjoy in Zimmerman. Whether you're in search of your favorite fast food combo, or a fun family sit-down spot, you'll love the local selection. Some favorites include delectable burgers and wraps at the Whistling Pickle, and spectacular calzones at the Zimmerman Bar and Grill.
The City of Zimmerman provides a host of resources for residents concerning recycling, composting, responsible disposal practices and resource conservation. Together with residents, the city hopes to increase quality of life and help to secure a brighter, more sustainable future.
ISD #728, Elk River Area Schools provides educational resources and services to Zimmerman residents of all ages. This district is known for crafting curriculum that provides personal growth in areas other than strictly academic. With extensive extra-curricular activities, and diverse programs the Elk River Area School District consistently produces test results above state and national averages.
The Tri-CAP Transit Connection, offered through the Sherburne County Transit Resources, provides public transportation services to the residents of Zimmerman. This schedules bus service provides rides from the city to Elk River, Big Lake, and Princeton.
Local Government
The Zimmerman City Council meets every first and third Monday of each month. This council is made up of five members, the Mayor and four at-large councilmembers, and is responsible for the administration of all city policies and ordinances.
City History
Located on the shores of Lake Fremont, Zimmerman was originally settled by that name. The village of Lake Fremont was incorporated in 1910, and over the years has maintained its agricultural roots. With the addition of the Great Northern Railroad line, the village prospered. In 1967 the village of Lake Fremont was incorporated into the City of Zimmerman. The name was chosen to honor Moses Zimmerman, who was instrumental in the decision to run a rail line to the community. Today, Zimmerman is home to approximately 5,000 residents.


text:
Is Susan Strong a human???
Idk if she is a human cause i remember when Finn felt under her hat, she had no gills, but the episode did not show full proof of Susan Strong, being a human, if any of you know more about the episode than me. Be free to leave some comments! :)


text:
BBC presenter Jeremy Vine was given a ticking off by police for speeding on his bike in Hyde Park.
The Radio 2 host was stopped for riding at 16mph in a 5mph zone on a cycle path.
The radio host was travelling at more than three times the limit for the area although he said he had not seen any signs informing cyclists of the permitted speed.
Vine - who posted a film clip of the moment he was stopped online - said he received a ribbing when he got to the BBC's New Broadcasting House.
He said: "When I got to the office and said I was radar-gunned at 16mph on my bicycle, producer said 'you are living on the edge'."
The midday show host told his followers on Twitter: "They said speed limit in Hyde Park is 5mph and I was doing 16mph. I apologised."
Although Mr Vine appeared to see the funny side, he later warned that cyclists would "return to the roads and mix it with trucks" if they are forced to travel at walking pace.
Vine later spoke about his experience on air, telling Radio 2 colleague Ken Bruce: "I haven't seen a radar gun for a long time so didn't expect one with the digit 16 on it.
"I feel embarrassed and I apologise. I didn't see any signs. I certainly will be cycling as slow as walking pace in future."
Mr Vine has campaigned for safer roads for cyclists and often posts videos showing the difficulties faced by those on two wheels.
Earlier this year he wrote: "Every morning when I leave the house, my wife says farewell in the manner of Japanese women who waved off pilot husbands in World War II.
"If nothing else, cycling in a major British city reminds you to make a will and tell your mum you love her.
"The biggest danger is not road layout but road users. The hostility of some drivers is mind-boggling."Reuse content


text:
One of my most distinct memories, if not the most distinct from my last trip to Malaysia is, of my aimless wanderings through the narrow lanes of George Town, the capital of Penang state. Be it the charming architecture, the rustic shops, the beautiful the graffiti, everything there was so charming in a rustic way. I could not have enough of this charming place even after roaming around the same places not once, not twice but thrice! There was hardly anything which I did not like there (if only the beer was a little cheaper though 😉 ). As I wandered through the narrow lanes of Georgetown, I could not help but imagine how would it have been life here like, half a century back! [More on Georgetown coming soon]
Malaysia was nowhere near the top in my list of south-east Asian countries that I wanted to visit.… continue reading


text: LINDAU, Germany, July 3, 2013 /PRNewswire/ -- Speaking at a panel held during the 63rd Lindau Nobel Laureate Meeting in Germany and hosted by Mars, Incorporated, Nobel Laureate and former US Secretary of Energy, Steven Chu, and Chief Agricultural Officer at Mars, Howard-Yana Shapiro, urged an audience of 150 of the world's brightest young researchers to seek multidisciplinary, cross-sector scientific collaborations in order to tackle interrelated and global resource issues, from climate change to nutrition security. (Photo: http://photos.prnewswire.com/prnh/20130703/NY42133 ) (Logo: http://photos.prnewswire.com/prnh/20110928/NY76189LOGO ) Steven Chu, Physics Nobel Laureate and Stanford University Professor, spoke directly to the young researchers: "We will continue to face interconnected challenges involving energy, agriculture and water that we have a moral obligation to confront. Scientific collaboration - and the determination of people like you - will play a key role in addressing that." Howard-Yana Shapiro spoke about Mars, Incorporated's involvement in the African Orphan Crops Consortium (AOCC), a cross-sector collaboration that aims to sequence, assemble and annotate the genomes of 100 traditional African food crops to improve their nutritional value, productivity and climatic adaptability. The data gathered by the AOCC will be made publically available with the endorsement of the African Union. "As one of the world's leading food manufacturers, Mars has a critical interest and role to play in the sustainable management of our global resources," said Howard-Yana Shapiro, Chief Agricultural Officer at Mars, Incorporated and Senior Fellow, UC Davis Plant Science Department. "Partnerships that bring together varied expertise are the best way to address the global issues facing us as a business, and society as a whole. That's why initiatives like the African Orphan Crops Consortium - that form uncommon collaborations across sectors and share pre-competitive data - are so important." The panel was moderated by Adam Smith, Editorial Director of Nobel Media, and also featured the views of Christina Heroven, a Biochemistry Masters student from the Freie Universitat Berlin. Speaking after the panel discussion, Christina Heroven commented: "It was great to be part of such a dynamic discussion, this panel - and the Lindau meeting more broadly - show the importance of dialogue and collaboration in science." This annual panel discussion and informal Q&A session, supported by Mars, Incorporated, serves as a platform for the exchange of new ideas and perspectives across the spectrum of scientific disciplines represented at the Lindau Nobel Laureate Meeting.


text:
Need a T4 Test in Bay Village, OH? Simply order online 24/7 and get a fast, affordable T4 Test at a lab near you (Find More Locations):
What is a T4 Test?
A T4 Test is a lab test that measures the T4 level. People in Bay Village, OH get the T4 Test for general screening or ongoing tracking.
How much does a T4 Test cost?
For current T4 Test prices in Bay Village, OH, please CLICK HERE.
What does a T4 Test lab report look like?
For a T4 Test sample lab report, please CLICK HERE.
We are delighted to offer the T4 Test in Bay Village, OH.
T4 Test in other cities near Bay Village, OH:Avon | Avon Lake | Berea | Brookpark | Cleveland | Columbia Station | Fairview Park | Lakewood | Middleburg Heights | North Olmsted | North Ridgeville | Olmsted Falls | Rocky River | Sheffield Lake | Westlake


text:
HATTIESBURG — Gov. Phil Bryant has set a Sept. 24 special election for a do-over in the Hattiesburg mayoral race.
Bryant's order today came days after Judge William Coleman ordered a new election because of questions about the way the June 4 election was conducted.
Former city council member Dave Ware, who ran for mayor as an independent, brought a court challenge after narrowly losing to Democrat Johnny DuPree, who has been mayor since 2001.
Ware contested the election results after DuPree was certified the winner with a 37-vote lead. Ware contends that absentee ballots had problems ranging from allegedly improper signatures to ballots that didn't state for which election the vote was cast.
Bryant said all mayoral candidates who ran June 4 will be on the ballot Sept. 24, unless they withdraw from the race. Shawn O'Hara, Nathan Jordan and Clyde Stewart also were on the June mayoral ballot as independents.
Voters must be registered by Aug. 26 to cast ballots in the new election.
The Hattiesburg City Council, meanwhile, met in closed session yesterday evening to discuss city attorney City Attorney Charles Lawrence's ordering Hattiesburg police to conduct a criminal background check of a man who had been scheduled to testify in the trial challenging the election results..
Council President Kim Bradley told the Hattiesburg American that the executive session involved questions directed at Lawrence over why he ordered a National Crime Information Center check of Ware supporter Arthur Smith. Bradley said he was constrained by the laws of executive session to comment further about the matter, but said: "No action was taken."
Lawrence told the Hattiesburg American in Sunday's edition that he ordered the background check because he believed Smith, whose absentee vote was lost, would perjure himself on the stand.
Smith filled out his absentee ballot for Dave Ware. However, a search of the ballots did not find an Arthur Smith vote. WDAM-TV reported that during the City Council's closed session, it was revealed in a court transcript that the Hattiesburg Police Department, on orders from Lawrence, did a background check on Smith. Smith was called to testify as a witness in the Ware v. DuPree trial, but he never showed up when subpoenaed July 26.
Forrest County District Attorney Patricia Burchell released a statement yesterday saying she is aware of citizens' concerns stemming from the mayoral election and trial.
"I have received numerous inquiries from members of our community as to what actions this office will take to investigate criminal activity. I want to assure the citizens of Hattiesburg that your concerns are not being ignored," Burchell said.
She said it would be "improper" to comment on any specific allegations or investigations during the ongoing electoral process.
"The office is committed to ensuring that all allegations of any type of criminal conduct within our community are investigated and properly handled," she said.


text:
U.S. Open Day 9 matches to watch
(UPDATE: Rain washed out play on Tuesday. These matches were rescheduled for Wednesday as part of a jam-packed order of play, weather permitting. For more previews of Day 10 matches, click here.)
Donald Young vs. Andy Murray (first match, Ashe): Unless someone spikes Murray's breakfast, this won't be a redux of their March clash at Indian Wells, where Young ousted the weary Scot 7-6 (4), 6-3 for the biggest win of his career. This is a different Murray in New York, as he admits he was stuck in a mental slump for months after his loss to Novak Djokovic in the Australian Open final. Sure, Murray's form can rise and dip from match to match, but Young doesn't have the power to bother the fourth seed. The American's game is not unlike the Scot's, reliant on movement and finesse to open up the court and go for winners. The difference here will be Young's serve, which is still a liability, and Murray's experience. You never know which Andy Murray will show up, but he has a pretty good track record once he gets past the first week of a Slam.
David Ferrer vs. Andy Roddick (first night match, Ashe): A battle of grinders, pitting Roddick's great serve against Ferrer's great return. Ferrer leads the head-to-head 5-3, and he beat Roddick in straight sets at Davis Cup in July in front of the American's hometown crowd in Austin, Texas. That loss will surely give Roddick the extra motivation he needs, but Ferrer is the in-form player. This one's going to have some rallies.
John Isner vs. Gilles Simon (first match, Armstrong): Big man vs. little man. Server vs. runner. You'd be hard-pressed to find a bigger contrast in styles and it's tough to know how it'll play out given the two have never met. Isner has quietly made it through his portion of the draw and is coming off a strong summer, while Simon has had to scrap and fight to make the fourth round, playing two five-setters and one four-setter. But his win over Juan Martin del Potro in the last round has to have buoyed him as he withstood a strong charge from the Argentine and wore down the big man. I suspect he'll be able to do the same thing to Isner. Sam Stosur vs. Vera Zvonareva (third match, Armstrong): Can Stosur bring the drama for the third straight match? The Aussie is breaking records left and right and playing the best tennis of her year, too. She's got a great chance to upset the second-seeded Zvonareva, as Stosur enjoys a 7-2 head-to-head record and a streak of seven straight wins (she hasn't lost since 2004). That said, they haven't met on hardcourts this year and their clashes on clay (Stuttgart) and grass (Eastbourne) were very tight three-setters. No. 9 Stosur is riding a wave of confidence going into this match, but if she blinks at all like she did against Nadia Petrova and Maria Kirilenko (blowing leads in both matches), Zvonareva will capitalize.


text:
Rife machines and cancer
This page tells you about Rife machines and cancer. There is information about
There are several websites claiming that the Rife machine can be used to treat a number of different conditions including cancer. Most of the claims on these websites are personal accounts. They don't have any scientific research to back them up.
A Rife machine produces electromagnetic energy in the form of electrical impulses. Electromagnetic energy is a combination of electric and magnetic energy that travels in waves. Examples include gamma rays, radio waves, ultraviolet radiation, X-rays and visible light.
The Rife machine was developed by Royal Raymond Rife in the 1920s. He was an American scientist. The machine is also called a Rife frequency generator. Rife and his supporters say that each disease or condition has its own electromagnetic frequency. They also say that finding that frequency and producing an impulse of the same frequency will kill or disable diseased cells.
To use the Rife machine you put electrical pads on either your feet or hands. These pads are attached to the machine, which produces the electrical impulses. You connect yourself to the machine for a few minutes a day, several times a week.
The Rife machine and other types of similar machines produce low energy waves. There is no evidence that the electrical energy the Rife machine produces can destroy cancer cells.
A search on the internet brings up different prices for the machine from a few hundred pounds to a couple of thousand pounds. Supporters say there aren't any side effects. But there have been reports of electrical shocks and rashes.
Before any new treatment or diagnostic tool can be used it goes through a long process of development. During this process the researchers test to check that it works, that it doesn't do any harm, and that the benefits outweigh the disadvantages.
The Rife machine has not been through the usual process of scientific testing. There is no evidence to show that the Rife machine does what its supporters say it does. There is also no evidence that it doesn't cause harm.
A randomised clinical trial is the only way to properly test whether any new treatment works.
Supporters of this treatment say there is reluctance within the medical community to support research into the Rife machine. And some people say there is a conspiracy to stop it being developed. But there have been some developments using electromagnetic energy to diagnose and treat a number of different diseases. And this research is ongoing.
Researchers have been interested in how electromagnetic energy can be used to diagnose and treat diseases for a long time. And there have been some developments that we know help diagnose and treat cancer. For example, X-rays, radiotherapy and magnetic resonance imaging (MRI) scans. These all use high energy waves and are specialist machines which are proven to work.
Some researchers have been looking into the effects of low level electromagnetic energy on cancer cells. They have found that these low frequencies do seem to affect cancer cells but not normal cells. However, this research is still at an experimental stage, and it's not clear exactly how it could work. And the electromagnetic frequencies used in this research are different from those produced by Rife machines.
The Rife machine has not been approved by the Food and Drug Administration (FDA) in America. And the American Cancer Society says that the FDA has warned companies who sell these machines not to make claims that have not been proven.
Rated 2 out of 5 based on 157 votes
Question about cancer? Contact our information nurse team


text:
Suede Feelings Crop Top
Great things come in small sizes. This ultra chic cross-over crop top is the perfect go-to piece for any parties or a girl's night out! Stylish suede finish with a classic V-neckline. Dainty shoulder straps with small cut outs for a sexy peek-a-boo effect. Style with matching midi skirt for an irresistible evening outfit!
- Self: Nylon-Spandex blend
- Lining: 100% Polyester
- Imported
- Model is wearing size S
- Runs true to size
- Machine wash cold


text:
SailNet Community (http://www.sailnet.com/forums/)
- C320 (http://www.sailnet.com/forums/c320/)
- - Yanmar 3YM 30 start battery (http://www.sailnet.com/forums/c320/72558-yanmar-3ym-30-start-battery.html)
Yanmar 3YM 30 start battery
We want to install a dedicated start battery in out C320 and use the 2 house bateries for storage. Any suggestions as to the requirements for the 3ym 30 or recomended a batery size? Thanks
I have a 3ym 30f, installed 7 years ago. Still starts first time from my dedicated 80 AH through a double pole switch and charges on the 1 or both settings.:)
Safe sailing
I have a 3YM30 in our Hunter. Installed a group 27 start battery, 600 CCA, 750 MCA, on an echo charger and 2 Group 27 as house bank on a 1,2, both switch. Typically I leave it on the house bank and start the engine from that bank. I only use the engine start battery if I draw down the house bank too far. It's happened a couple of times, but have the reserve engine battery as back-up.
That being said you can just use the house bank to start and your new batt of at least 400CCA for reserve. Might as well go for something in the 600+CCA class as even small group 24's will do this..
Are you wiring a dedicated engine start circuit or using a 1/2/BOTH/OFF switch?
|All times are GMT -4. The time now is 07:15 AM.
Powered by vBulletin® Version 3.8.7
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
vBulletin Security provided by vBSecurity v2.2.2 (Pro) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.
User Alert System provided by Advanced User Tagging v3.1.0 (Pro) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.
(c) Marine.com LLC 2000-2012


text:
Doctor insights on:
Burping And Pain In Left Side Under Lower Ribs
When i burp I have a pain under lower rib left side what might that be?
From stomach?: Burning is due to excess air that you swallow during eating and drinking trying to come out of your stomach. The area you describe is where your stomach usually is when distended with food and water. Pain is felt due to muscles in between ribs contracting along with your diaphragm to expell air out in to esophagus. ...Read more
Belching (Definition)
Belching (big burps) is a symptom in which a person forces air noisily from his stomach out through his mouth. Belching can be due to too much air or gas in the stomach. Sometimes, people purposely swallow air and belch it back ...Read more
Burning sensation in lower left side below rib cage and goes to the lower left back side. It sometimes stingy/itchy and I am burping alot. Cause?
GERD a Possibility: GERD (Gastro Esophageal Reflux Disease) could be causing your symptoms. This is when acid in the stomach goes up into the esophagus and causes pain. It's also possible you have a hiatal hernia, which can cause similar symptoms. I recommend you see your family doctor when possible. ...Read more
Pain in left side of chest and back and under left side ribs frequent burping difficulty swallowing feels like food has hard time going down in chest?
See a doc: This might be related to gerd (gastroesophageal reflux disease) and could be helped with an acid blocker such as omeprazole. You could try that for a couple of days. If it persists, though, probably best to see your physician. ...Read more
Can pain on the left side of rib cage be cause by a gas build up? I had pain for 3 days, after i burped it reduced drastically? Was that it?
Yes & it sounds like: U made the diagnosis Urself. There R OTC remedies 2 help with this. ...Read more
Ihave random digestive noises,off bowels&random shooting pains on my left side and under my left rib.No reflux or burping but gas.vagas nerve related?
See your doc: Bowel problems range from bad food, to lactose intolerance. You can try eliminating gassy foods such as milk, cabbage family, beans, etc or try adding digestive aids, but a colonoscopy would tell if the tissues are healthy or not. Bowel habits, frequency of BMs, and diet are very important for a healthy digestion. GI docs, or your FP can answer your questions ...Read more
Pain down left side, ribs and back. Burping a lot . Some burning feeling in middle of chest . Tiny black specks in stools, but stools are a normal?
GERD?: You may have gastro-esophageal reflux disease...See a primary care doctor first... ...Read more
Pelvic pain on right side, day after ovulation also sharp pains below left rib and in upper back. Excessive burping as well?
Ruptured cyst: May have bled a little into the cyst where you ovulated from, causing pains in the right pelvis. The pains in the upper abdomen and upper back may be from bloating, gastrointestinal issues (the burping). ...Read moreSee 1 more doctor answer
Can excessive gas cause rib and back pain? Sharp pains under left rib, down left side and mid to upper back . Some burping and flatuence
Yes it can: Excessive gas in the bowels can cause pressure upwards and backwards. Pressure upwards effects the diaphragm which attaches to the ribs. Pressure backwards can impact the back. Drinking warm tea and gently massaging the belly may help move the gas along. ...Read moreSee 1 more doctor answer
Pain down left side, ribs and back. Burping a lot . Some burning feeling in middle of chest . Tiny black specks in stools is it excessive gas?
Melenic specks?: Dark-colored stools may result from iron supplements, foods you eat (like a lot of spinach), substances (like peptobismol), but most significantly suggests bleeding. When blood is denatured by acid in your stomach, as from ulcers, stools will turn tarry ("melena"). Blood may also be ingested from nose bleeds, bleeding gums. Persistent melena with your dyspeptic symptoms however warrants md visit. ...Read more
Constant lump sensation in left side of throat, frequent burping, feels like liquids get stuck going down chest leftside, pain under left side of rib?
Get evaluated.: Anytime you feel a sense of food or fluids "sticking" at a specific place, I am concerned that there may be a narrowing in the esophagus. This may be as simple as irritation from a capsule that got stuck to something more complicated like a tumor. Needless to say, you need the information. Your doctor may refer you to a gastroenterologist, or send you for some imaging studies first. Good luck. ...Read more
- Talk to a doctor live online for free
- Left side pain under ribs and lower back
- Pain in lower left side under ribs
- Pain lower left side under rib cage
- Ask a doctor a question free online
- Indigestion pain under left rib burp
- Pain under left rib cage and burping
- Pain left side under rib cage lower back
- Pain in the left side under ribs
- Talk to a gastroenterologist online


text:
Two 「黒ー33」Mature
"I doubt this cat even exists. Cats can't talk, after all. Poor thing."
"So…" he said.
"It doesn't realize it's dead does it? Can it say anything else?"
The cat, as if understanding Noa, looked up, opening it's mouth.
She stood and went to make coffee, handing the cat to Noa, who, fumbling, sat back, eliciting another tirade from the cat. When it calmed down, sitting it between his legs, he began to watch her from his bedroom. He watched the sway of her hips, those tight pants drawing up, her shape distracting, even as she pulled that sweater back down, scratching her head. Seconds later he smelt that familiar cheap coffee smell, that strange, off-color burnt smell you'd normally associate with burnt coffee that'd been sitting on the burner for too long. To avoid this, he'd normally brew it one cup at a time. Filters are cheap, he reasoned, but coffee wasn't, so why waste it? The cat was getting impatient, shouting off another report.
["Sumimasen, Wakarimasen!"]
Noa looked at it.
"What're ya even trying to say anyway?" he asked as she entered again, two cups on a platter, steaming, and she sat down with it, sitting it on the squat wooden table in front of them.
"Your coffee is awful Noa." She said, smelling it.
"What? It's fine. Cheap shit is always awful."
"That's not the point." She said, moving backwards, crossing her legs. "Even if I where a poor dumbass like you I'd still buy the top shelf stuff. You're not doing much with what you've got anyway.
"You're employed by the United Authority though, don't call me a dumbass, I'm barely scraping by."
"Why'd you move out? You're only twenty two."
The cat was asleep, at least that's what he'd thought. It'd stopped moving for a short while, prostrate over Noa's thigh.
"Because it was noisy. Not the kind of noise you'd understand, though. Just them being around bugged me for some reason. I had to leave. Would've shot them otherwise."
"It's that bad?"
"Nah, I'm exaggerating. I'm not mentally sound, don't get me wrong. You know about my problems but-"
She cut him off, finger to his lips.
"Don't worry about that right now." She said, taking a sip from her cup.
"Why do you buy such awful coffee?"
He groaned, got up and walked over to his bed, some squat, metal thing with a pad over some springs. Sat down, laying back.
"I'm tired, take that thing and go home. Your sister is useless anyway, probably starving."
"Sadie isn't that smart but she's not useless, and no, remember, it's not a real cat. There's no telling what it could be either." She said through another quick sip, eyes watering, coughing.


text:
In a message dated 11/17/2006 7:41:50 A.M. Central Standard Time,
email@example.com writes:
<<Good to see. >>
This one went through so I'll try the photo again! This is a red
rebloom seedling from Radiant Energy X Summer Radiance. 1814-3Re.
________________________________________________________Betty W.
in South-central KY Zone 6 ---Bridge In Time Iris Garden@website:Where
the seeds are in the pots once again! www.thegardensite.com/irises/bridgeintime/Reblooming Iris - Home Page iris-photos archives iris-talk archives AIS: American Iris Society website
<<Good to see. >>
US News
Get the latest
national news now
"The 9"
Daily count down
of top Web finds.
Free Blogging
Share your views
with the world.


text:
Wrestling Forum : WWE, TNA, Indy Wrestling, Lucha Underground, Women of Wrestling Forums (http://www.wrestlingforum.com/forumindex.php)
- GFX Archive (http://www.wrestlingforum.com/gfx-archive/)
- - CM Punk Sig Request! (http://www.wrestlingforum.com/gfx-archive/660274-cm-punk-sig-request.html)
CM Punk Sig Request!
Hey guys, I'm looking for a simple Punk sig from any of you clever bots out there! Basically what I'm looking for is this image: http://www.enfinity1productions.com/...k_Bio_0003.jpg
I wanted that image above to be given a gritty feel to it, most black, secondary colour white.. and in a white font on the black background all I want is "#BITW", then just to put my username in the corner or something! I'll give credit to whatever sig I use and you'll get a rep!
Re: CM Punk Sig Request!
This is my first attempt at doing a sig for someone, hope its alright!
EDIT: tried to use the # but it didn't really look rugged because the font wouldn't work.
Re: CM Punk Sig Request!
Re: CM Punk Sig Request!
Both AWESOME sigs guys.. but I think I'll use nemisisdivina's for now!
|All times are GMT -4. The time now is 06:48 PM.
Powered by vBulletin® Version 3.8.7
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
User Alert System provided by Advanced User Tagging (Pro) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.
vBulletin Security provided by vBSecurity v2.2.2 (Pro) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.


text:
It only takes a minute to get started. Fill out our uncomplicated sign up form and login to the site.
This step is discretionary but will seriously raise your chances of your profile being viewed by interested members. Write a little about yourself, your pasttimes and upload your best photo.
Utilise our website's sophisticated search to discover over 60s members who suit your criteria. Or just look through listings and discover singles that arouse your interest.
Discovered someone who ticks all the boxes? Send a note to them or just a wink and see what happens next. With a little luck you'll be on your way to discovering that special someone for you.
It doesn't take long


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Woodchuck <woodchuc...@yahoo.com>
|RE: Redirecting Tomcat to IIS (UNCLASSIFIED)
|Tue, 14 Jun 2005 18:03:08 GMT
hihi Ed, i have windows 2003 server, IIS 6.0, Tomcat 5.5.9 and i have redirection working fine. i used to have windows 2000, IIS 5.0, Tomcat 4.1 and i also had redirection working fine in that setup as well. my question to you is, how are you setting up the JK connector? are you doing it manually? or are you using the setupJK204.exe program? i ran into problems setting up the JK connector manually, even though i thought i covered everything (including registry). but i found using the setup program to be flawless as long as you always uninstall the previous JK installation before installing the new one (ie. use windows' add/remove programs to uninstall whenever you add a new Tomcat webapp and need redirection for example). to be absolutely safe, you *need* to reboot server after uninstalling JK. after rebooting then you can install JK again. this is my process and it has worked 100%. the setup program can be found here: http://www.shiftomat.com/opensource/ hth, woodchuck --- "Young, Ed CONT Anteon" <firstname.lastname@example.com> wrote: > Classification: UNCLASSIFIED > Caveats: NONE > > I haven't removed any files and the default files and the Default > page > is listed above the iisstart.htm. My application is using tomcat now > but > needs to be redirected to IIS. The isapi filter is green and pointing > up. I have also checked the registry settings to ensure the correct > paths are specified. > > -----Original Message----- > From: Peter Crowther [mailto:email@example.com] > Sent: Thursday, June 09, 2005 8:26 AM > To: Tomcat Users List > Subject: RE: Redirecting Tomcat to IIS (UNCLASSIFIED) > > > From: Young, Ed CONT Anteon [mailto:firstname.lastname@example.com] > > I have an issue with redirecting my web application from Tomcat > > 4.1 to IIS 6.0 on Windows 2003 Server. I have followed the steps in > > configuring the redirect but once I try to access the page using > port > > 80, I get an "Under Construction" error. How can this be rectified? > > If you access the URL to which you're redirecting directly (i.e. > without > going through the Tomcat application and it issuing the redirect), > does > it work? > > What's the default page for the IIS6 directory? Have you removed > iisstart.htm from the directory in which you've deployed the IIS6 > files? > If you look at the list of start pages on the 'Documents' tab of your > IIS6 sites, property pages, is that default page listed above > iisstart.htm? > > - Peter > > --------------------------------------------------------------------- > To unsubscribe, e-mail: email@example.com > For additional commands, e-mail: email@example.com > > Classification: UNCLASSIFIED > Caveats: NONE > > > --------------------------------------------------------------------- > To unsubscribe, e-mail: firstname.lastname@example.com > For additional commands, e-mail: email@example.com > > __________________________________ Discover Yahoo! Use Yahoo! to plan a weekend, have fun online and more. Check it out! http://discover.yahoo.com/ --------------------------------------------------------------------- To unsubscribe, e-mail: email@example.com For additional commands, e-mail: email@example.com
|View raw message


text:
The electoral register - open
The open register is an extract of the electoral register, but is not used for elections. The personal data in the register must always be processed in line with data protection legislation. It can be bought by any person, company or organisation. For example, it is used by businesses and charities to confirm name and address details.
The personal data in the register must always be processed in line with data protection legislation.
Your name and address will be included in the open register unless you ask for them to be removed. Removing your details from the register would not affect your right to vote.
If you wish to withhold your details from the open/edited register or have your details reinstated in it, please complete and return the open register change request form.
Who uses the open register?
Users of the open register include:
- businesses checking the identity and address details of people apply for their services such as insurance, goods hire and property rental, as well as when they shop online
- businesses selling age-restricted goods or services, such as alcohol and gambling online, to meet rules on verifying the age of their customers
- charities and voluntary agencies, for example to help maintain contact information for those who have chosen to donate bone marrow and to help people separated by adoption to find each other
charities, to help with fundraising and contacting people who have made donations
- debt collection agencies when tracing people who have changed addresses without telling creditors
- direct marketing firms when maintaining their mailing lists
- landlords and letting agents when checking the identity of potential tenants
- local councils when identifying and contacting residents
- online directory firms to help users of the websites find people, such as when reuniting friends and families
- organisations tracing and identifying beneficiaries of wills, pensions and insurance policies
- private sector firms to verify details of job applicants
Was this page useful?Yes No
Thank you. You can provide further feedback on this page using our feedback form.


text:
Physical and mental health
17 February 2011
The headline target of the Council of Australian Government's Closing the Gap agenda is the elimination of the difference between Indigenous and non-Indigenous life expectancy in Australia. While this in and of itself is a worthwhile (if difficult to achieve) aim, life expectancy or even Quality Adjusted Life Years represents just one aspect of physical and mental health. Instead, the World Health Organisation defines health as 'not only the absence of infirmity and disease but also a state of physical, mental and social wellbeing'. This lecture begins with a discussion of Indigenous notions of health. We will


text:
Acne. With.red.marks
I have pimples since 7 8 days with red marks..have taken med but not working applied clindamyccin n taken fluconazole
Answers (2)
Like the answers? Chat privately for 24 hours with the doctor of your choice
Dr. Govind Suresh Mittal
Trichologist 6 yrs exp Bangalore
Free 3 day follow up
98 %Chat Now
₹350 for 24 hrs
Hi. Greetings. Is this the first time you have head acne ?? Acne is a chronic problem. You may need a few medications and lifestyle changes for long term improvement. The following would help you : 1. Daily use of gel based sunscreen in day time of SPF 30 applied half hour before sun exposure to prevent darkening of spots. 2. Daily bed time use of retinoid cream 3. Antibiotic agents short course- prescribed by dermatologist for controlling out bursts .salicylic acid based or benzoyl peroxide based face wash for oily skin. 4. Evaluation by dermatologist for any other trigger or need for oral retinoids- this will indeed help but should be taken only if necessary and under monitoring. Also salicylic acid Peel if needed 5. Procedures like microneedling rf, fractional lasers or just microneedling for scars are helpful and necessary. Chemical peels for acne and spots. 6. Kindly read my blog on daily skin care on Practo health feed. Daily fitness work out and loads of fruits and veggies will help loads in improving your skin. You may Send further queries/ pics of lesions on private follow up chat. Hope this helps. Warm regards.
Flag this answer
2/2 people found this helpful
Was this answer helpful?
Dr. Rajib Roy
Trichologist 8 yrs exp Kolkata
Free 3 day follow up
Chat Now
₹250 for 24 hrs
Hi. I can understand your concern. Well fluconazole is not meant for Acne. Clindamycin is fine but only clindamycin will not help. Get a detailed work up done by Dermatologist near to you. Thank you
Flag this answer
2/2 people found this helpful
Was this answer helpful?


text:
Need an online lab test in Zionsville, IN? Simply order the online lab test you want and get tested at a lab in Zionsville, IN (Find More Locations):
How much does an Online Lab Test cost?
Our Online Lab Test prices include the doctor's order that you need to get tested in Zionsville, IN, all lab fees and taxes, and a pdf copy of your results. For current prices in Zionsville, IN, please CLICK HERE.
What does a report for your Online Lab Test look like?
For a sample report of one of our popular panels, please CLICK HERE.
We are glad we can help people in Zionsville, IN get an Online Lab Test.
Online Lab Test in other cities near Zionsville, IN:Avon | Brownsburg | Carmel | Fishers | Indianapolis | Kirklin | Lebanon | Lizton | Noblesville | Pittsboro | Plainfield | Sheridan | Speedway | Westfield | Whitestown


text:
Contributeto QRZ
Login is required for additional detail.
QSL: eqsl
Email: Login required to view
Ham Member Lookups: 1526
6170653 Last modified: 2015-07-16 00:21:07, 4361 bytes
Login Required
Login is required for additional detail.
You must be logged in to file a report on this page
Please login now...
Book Totals:
741 qso's
235 confirmed
Get a free logbook at QRZ.COM


text:
CFTC: USD longs declined
According to the latest COT (Commitments of Traders) report, the net long USD positions fell from from $15.95 billion on June 10 to $12.19 billion on June 17. The USD dollar bullish bets showed a first decline after five straight weekly increases. Despite the weekly decline, the overall USD position remained on the bullish side for a 6 consecutive week.
Large speculators bets in favor of GBP, JPY, CHF and CAD increased, while EUR, AUD and NZD positions declined.


text:
You are currently using an unsupported web browser.
We support the latest versions of these browsers:
If you have any problems while attempting to download a browser, you should contact your organization's system administrator.
For any questions related to Cvent, feel free to contact our Customer Care team.
Northstar Ratings are powered by Northstar Travel Media and are based on objective and unbiased evaluations from experienced editors that individually analyze, research, and evaluate hotels. Hotels and suppliers are not required to pay any fees or pay for memberships to have their property rated.
Northstar Ratings are powered by Northstar Travel Media. Please email email@example.com if you have any questions or comments regarding the rating of an individual property.
The rich Portuguese heritage of Maputo, Mozambique, flows through the décor, detail and hospitality of Southern Sun Maputo. Located on the Maputo beachfront, this hotel is a popular haven for business and leisure guests, and is a mere 7km from the Maputo International Airport. Offering a 158...
You must be logged in to view contact information for this supplier. Sign up for a free Cvent account today.


text:
[uncountable] (symbol Pb) a chemical element. Lead is a heavy soft grey metal, used especially in the past for water pipes or to cover roofs. Lead exposure can be harmful to everyone, especially young children and babies.
lead noun definition in Materials and properties topic from the Oxford Advanced Learner's Dictionary
Explore synonyms and entries related to Materials and properties
Explore other topic groups related to Materials and properties


text:
« « PREVIOUS ARTICLEFree Web Hosting Service
Free Webspace
|Date Added: Nov 21, 2012
|Author: Ross Barringer
|Category: Computers and Internet: Internet: Web Design and Development: Hosting: Free
When picking out this internet host, take into consideration the amount regarding the host apr's. Some hosts charge on your per-year foundation maybe some might be month-to-month. Glimpse for a host that expenditures a real median of involving $5 per period as this will likely provide you with some stellar worth to produce your personal $ versus a quite free web hosting which could as opposed to offer you seeing that many alternatives.
Free hostings provide a decent environment to test different webmaster techniques. You do learn and play around many things well before switching to covered hosting. Immediately your site is literally published online, the public don't get on to stop working on the it's design! Keeping on good of your website will help your to ensure it actually is always current. This does not solely mean that yourself have to consistently change it, if you will has to preform various regular updates. This is one specific must especially the actual event that you host clips or work by having current events. Updating a website is quite numerous from updating a blog. Now there is a masse of work involved.
Also there are numerous web hosting services coupled with free web hostsing services on your the web. A great contend of the free web hosting online websites offer adequate web hosting for per price in lieu of the generic, free version. A ones you definitely not pay for have their establish along with your ultimate unique domain name in the address. If you have a business, a potential owner might think you'll are not successful enough to have your own website, and turn besides to find anybody else. Who's is quite nearly impossible to suggest the exact amount within bandwidth that that you require for an individuals website. This can solely depends forward the design along with your website, those target audience as the total assortment of the targeted visitors you are in the position to pull based on your website.


text:
Upstream / Downstream
Explore pathways related to this product.
Discovery Through Validation
To Purchase # 62255S
|100 µl (50 tests)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (PE Conjugate) #62255
Flow cytometric analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (PE Conjugate) (green) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (red).Learn more about how we get our images
Gallery: Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (PE Conjugate) #62255
Flow Cytometry General Protocol
If using whole blood, please follow the Flow Cytometry Whole Blood Protocol.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 16% Formaldehyde (methanol free).
- 100% methanol.
- Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.
B. Fixation
- Collect cells by centrifugation and aspirate supernatant.
- Resuspend cells in 0.5–1 ml 1X PBS. Add formaldehyde to obtain a final concentration of 4%.
- Fix for 10 min at 37°C.
- Chill tubes on ice for 1 min.
- For extracellular staining with antibodies that do not require permeabilization, proceed to immunostaining (Section D) or store cells in PBS with 0.1% sodium azide at 4°C; for intracellular staining, proceed to permeabilization (Section C).
C. Permeabilization
NOTE: This step is critical for many CST antibodies.
- Permeabilize cells by adding ice-cold 100% methanol slowly to pre-chilled cells, while gently vortexing, to a final concentration of 90% methanol. Alternatively, remove fix prior to permeabilization by centrifugation and resuspend in 90% methanol as described above.
- Incubate 30 min on ice.
- Proceed with immunostaining (Section D) or store cells at -20°C in 90% methanol.
D. Immunostaining
NOTE: Account for isotype matched controls for monoclonal antibodies or species matched IgG for polyclonal antibodies. Count cells using a hemocytometer or alternative method.
- Aliquot 0.5–1 x 106 cells into each assay tube (by volume).
- Add 2–3 ml incubation buffer to each tube and wash by centrifugation. Repeat.
- Resuspend cells in 100 µl of diluted primary antibody (prepared in incubation buffer at the recommended dilution).
- Incubate for 1 hr at room temperature.
- Wash by centrifugation in 2–3 ml incubation buffer.
- Resuspend cells in 0.5 ml PBS and analyze on flow cytometer; alternatively, for DNA staining, proceed to optional DNA stain (Section E).
E. Optional DNA Dye
- Resuspend cells in 0.5 ml of DNA dye (e.g. Propidium Iodide (PI)/RNase Staining Solution #4087).
- Incubate for at least 30 min at room temperature.
- Analyze cells in DNA staining solution on flow cytometer.
posted July 2009
revised September 2013
Flow Cytometry Whole Blood Protocol
If using cell lines, please follow the Flow Cytometry General Protocol.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 16% Formaldehyde (methanol free).
- Triton™ X-100: To prepare 50 ml of 0.1% Triton™ X-100 add 50 μl Triton™ X-100 to 50 ml 1 X PBS and mix well.
- 50% methanol.
- Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.
B. Preparation of Whole Blood (fixation, lysis, and permeabilization) for Immunostaining
- Aliquot 100 μl fresh whole blood per assay tube.
- OPTIONAL: Place tubes in rack in 37°C water bath for short-term treatments with ligands, inhibitors, drugs, etc.
- Add 65 μl of 10% formaldehyde to each tube.
- Vortex briefly and let stand for 15 min at room temperature.
- Add 1 ml of 0.1% Triton™ X-100 to each tube.
- Vortex and let stand for 30 min at room temperature.
- Add 1 ml incubation buffer.
- Pellet cells by centrifugation and aspirate supernatant.
- Repeat steps 7 and 8.
- Resuspend cells in ice-cold 50% methanol in PBS (store methanol solution at -20°C until use).
- Incubate at least 10 min on ice.
- Proceed with staining or store cells at -20°C in 50% methanol.
C. Staining Using Conjugated Primary Antibodies
NOTE: Account for isotype-matched controls for monoclonal antibodies or species matched IgG for polyclonal antibodies.
- Add 2–3 ml incubation buffer to each tube and rinse by centrifugation. Repeat.
- Add primary antibodies diluted as recommended on datasheet or product webpage in incubation buffer.
- Incubate for 1 hr at room temperature.
- Wash by centrifugation in 2–3 ml incubation buffer.
- Resuspend cells in 0.5 ml PBS and analyze on flow cytometer.
Reference: Chow S, Hedley D, Grom P, Magari R, Jacobberger JW, Shankey TV (2005) Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations. Cytometry A 67(1), 4–17.
posted November 2008
revised September 2013
|Flow Cytometry
Tri-Methyl-Histone H3 (Lys4) Antibody (PE Conjugate) detects endogenous levels of histone H3 when tri-methylated on Lys4. This antibody shows some cross-reactivity with histone H3 that is di-methylated on Lys4, but does not cross-react with non-methylated or mono-methylated histone H3 Lys4. In addition, the antibody does not cross-react with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, S. cerevisiae Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3, in which Lys4 is tri-methylated.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751.
The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).
1. Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
2. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
3. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
4. Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
5. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
6. Shi, X. et al. (2006) Nature 442, 96-9.
7. Wysocka, J. et al. (2006) Nature 442, 86-90.
8. Wysocka, J. et al. (2005) Cell 121, 859-72.
9. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063.


text:
DISCLAIMER: "Ugly Betty" is copyrighted to ABC Studios. I retain rights to the plot, but not the characters. This story is meant for enjoyment purposes only. No infringement is intended.
AUTHOR: Katrina
SYNOPSIS: AU. Gio/Betty. Halloween Challenge – Y is for Yarling. Betty heads home on Halloween night and meets an old friend.
A long, drawn out howl sliced through the evening air causing Betty Suarez to come to a sudden halt. She glanced around nervously then continued hurriedly down the sidewalk towards her house.
The blackness of the night enveloped her in its cold embrace as more unearthly baying sounded from somewhere behind her. Betty's steps faltered again just as a street lamp overhead flickered ominously before going out.
"Great," she muttered to herself as she anxiously looked over her shoulder. Seeing nothing, she took a deep breath and tried to steady her nerves.
"Don't be silly, Betty," she scolded herself sternly. "It's just Mrs. Patterson's dog making all the noise and street lamps blow all the time. Just because it's Halloween, it doesn't mean that anything creepy is going on…"
The yarling came again, closer this time, making her gasp and turn around in fright. Breathing rapidly, she scanned the empty street and shook her head as she took a couple of steps backwards before turning and hurrying off again, a little faster this time.
The lateness of the evening meant that the majority of the houses were in darkness as most families were already in bed. That was trouble working for Mode, there were a lot of unsociable hours.
She was so engrossed in reaching her home that it took her a couple of minutes to register the fact that she could hear footsteps from behind. Glancing over her shoulder, she frowned and her heart started to race slightly when she saw that no-one was there.
Turning back, the unmistakeable sound of footsteps came again and her heart began to thud heavily in her chest as fear started to set in. Determinedly staring straight ahead, she quickened her pace even more, breaking into a trot as the welcoming glow of lights from her family home beckoned her on.
Footsteps sounded once more, echoing loudly in the night. With a frightened yelp, Betty panicked and broke into a full out run. Her ears were filled with the noise of her own heavy breathing and somebody…or some thing…running after her.
Just as she reached the gate of her house, a hand clamped down on her shoulder causing her to let out a scream of fear and surprise. Spinning around, she blindly lashed out at her assailant and swung her bag around, hitting them squarely on the head.
"Let me go!" she shrieked as she kicked out at the offenders shin at the same time.
"Ow! Damn it, Suarez, that really hurt!" groused a voice she recognised instantly.
She stilled and blinked in confusion. "Gio? But…What are you doing here?" she queried in amazement. Suddenly she frowned then added irritably, "And why were you sneaking up on me like that?"
The young Italian deli owner rubbed his head and grimaced slightly. "I wasn't sneaking," he denied in annoyance. "I called your name but you didn't hear me. Must be all that hair."
Betty gave her one time beau a withering look then let out a sigh. "I'm sorry for hitting you," she mumbled half-heartedly.
"I should've shouted louder," he conceded wryly.
There was a moments pause, then Betty ventured quietly, "So, how was Rome?"
Gio shrugged and smiled. "Let's just say I'm not the same person I was before I left."
Betty nodded and looked down at the ground briefly as his words sunk in. He was telling her that he no longer felt anything for her and she felt an odd pang of loss at that knowledge.
As she lifted her gaze to meet his once more, the clouds above moved to reveal the bright glow of a full moon. Gio drew in a sharp breath and stared up at the sight, seemingly mesmerised.
Betty stared at his rapt face and saw that he looked rougher around the edges than he used to. His hair was ruffled in an untidy but oddly appealing way and he definitely looked as though he needed a shave.
She turned away and headed up the path. "Come on," she urged as she opened the front door to her home. "I'll make you a coffee and something to eat." She entered the house leaving the door open in invitation for him.
Gio closed his eyes for a moment and took in a deep breath, then exhaled slowly. Opening his eyes, the normally dark brown orbs now glowed bright yellow. With a feral smile that exposed ever elongating sharp teeth, he licked his lips in anticipation and walked towards the house.
"Thanks, Suarez," he growled as he ambled through the doorway. "I fancy a bite."
Slamming the door closed behind him, Betty's sudden scream was soon drowned out by the haunting sound of a blood-curdling howl.


text:
Internlelo.com understands you care how information that you provide is used and shared, and we appreciate your trust that we will do so carefully and sensibly. By visiting Internlelo.com, you are accepting the practices described in this Privacy Policy.
Internlelo.com is not intended for children under 13 years of age. We do not knowingly collect personal information from children under 13.
Internlelo.com may use social media account linked commenting system for users to comment on the content posted on Internlelo.
While this Privacy Policy states our standards for maintenance of data and we will make efforts to meet them, we are not in a position to guarantee these standards. There may be factors beyond our control that may result in disclosure of data. As a consequence, iBlazon Services Private Limited (i.e. Internlelo.com) disclaims any warranties or representations relating to maintenance or nondisclosure of data.
We may make changes to this Policy from time to time. Your continued use of any part of the site or services constitutes your acceptance of such changes. You should visit this page periodically to review this Agreement.