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Hanako and the Terror of Allegory
Wednesday, June 9, 2010 6/09/2010
Labels: Manga , View Comments
Labels: Manga , View Comments
Hajimemashite! It's Silver-chan! This review is for 'Hanako and the Terror of Allegory'. (Disclaimer: These are my views, *squish*, and I'm sure everyone's views are different.)
Hanako and the Terror of Allegory Vol. 1 by Sakae Esuno
Who hasn't heard an urban legend? Scary stories that we tell each other for fun. But what if urban legend's were true? What if by hearing and believing in them, you make them real?
Ever hear about the one about Axe Man? The man that stays under your bed with an axe and kills you after you've fallen asleep? Well the axe man is under Kanae's bed, she can't make him leave, and she can't let herself fall asleep. So Kanae seeks the help of a special kind of detective agency to take care of her little problem. Enter in Aso Daisuke, a detective who would rather have cases like finding lost cats or following a cheating husband than deal with supernatural problems. Aso has a few problems of his own as well. He counts his hiccups and why does he have a strange girl living in his bathroom?
I would rate this 4 out of 5. Lets start with the bad (though its not that bad) shall we? Ok, this first volume has three urban legends for your creepy reading fun. Great, but they all seem to be Japanese urban legends, so some of my friends and maybe yours will be very confused by reading this. The last story breaks off or should I say ends in the middle of a another twist to the end of the last story. (Odd end to me, but hey, what do I know?)
On to the good. While the art was a bit more simple than I like; it was well drawn. The characters showed great emotions; you could feel their fear or embarrassment. It was unrepentantly funny; the running gag about Aso's porn was well done. Also it says horror but it's not. It's just creepy enough that you get goose bumps but not nightmares.
All in all, Hanako and the Terror of Allegory is a spooky, engaging read, and it keeps you interested. I am definitely looking forward to the next volume. I am glad my friend at the book store suggested that I read this.
Until the next review. Ja ne'.


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Sweet Destiny
Release Date: 17 March 2008 | Certificate: Suitable for 12 years and over
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With her mother in meltdown and her ever-so-slightly alcoholic father more concerned with concealing his naughty secret, Gugan's impending motherhood becomes the scandal of the year and divides her neighbourhood in two. The question is who will realise first that it's family, rather than pride that's most important?
On The Set , Cast and Crew Interviews , Also Available Trailers
Release Date: 17 March 2008
Certificate: 12 - Suitable for Persons Aged 12 or Over
Region 2
Sound: Dolby Digital
Main Language: English
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Catalogue No: SBX400

subset: commoncrawl


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Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG / IgA / IgM (H+L) Secondary Antibody, HRP conjugate (Product # A-10668) at dilutions 1:500 (Fig. 1), 1:1,000 (Fig. 2) and 1:2,000 (Fig. 3). A 22 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity
|Host / Isotype
|Goat / IgG
|Secondary Antibody
|IgG gamma 1
|Affinity chromatography
|Storage buffer
|0.1% thimerosal
|Storage Conditions
|Antibody Form
|Whole Antibody
|Tested Applications
|Dilution *
|1:500 to 1:2,000
|Immunohistochemistry (IHC)
|1:500 to 1:2,000
|Western Blot (WB)
|1:500 to 1:2,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Storage and reconstitution: reconstitute in 200 µl PBS, pH 7.2, to yield a 1 mg/mL stock solution. Store solution at 2-6°C with the addition of thimerosal to a final concentration of 0.02%. For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


text: Parallel Watermarking of Images in the Frequency DomainDorothy Bollman, Alcibiades Bustillo, Einstein MoralesScalable Computing: Practice and Experience2015While the internet has made it possible for the consumer to easily obtain images, audio, video, etc. in digital form, it has also made it easier to illegally obtain copyrighted material. Digital watermarking is a partial solution to this problem. Embedding a watermark in a legal version of material can help the copyright owner to identify who has an illegal… (More)


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Free AtlastMature
"Oh! How it feels good to be free... free from the stupidity of my past. But I don't know until when I will be stupidity-free."
After i got out of that place we called batcave, i found out that my parents adopted my daughter, because they said i was still young and uncapable of being a mother, so i never got achance to take care of her. And i feel pitty for her. But maybe my parents does know what was best for us. So I just let them be fully in charge of her. And me? I don't know.
I Do not know until when I will still be facing my own shadows and Move forward the right path, for I am only human with hungers in life, searching for foods to feed my own soul without having no one to tell me which foods will feed my hungers without having stomach aches.
There are so many things I've learned from my past. That I should become wiser to choose whom to be friends with and who to give my heart to. But I'm not sure if I can do that. I know myself well. I don't choose people based from what I see in them, because I don't easily judge people. I get to know them first and I don't just get rid of them, if I don't like them, I still treat them properly, of course. But I guess that is the main problem in me. I easily trust. Then that was the start of my way more challenging life still as a teen at 18. The legal age. Quite matured enough to know what is right from wrong. Surely, I do obeyed my parents' house rules. I follow what they want me to do. I stay at home and do house chores. I go out with them to watch movies. I don't entertain suitors. And I don't have a boyfriend but I do have "friends with benefits" guy friends secretly, which doesn't count as my boyfriends. Quite enjoying "FREEDOM" huh?
I don't know why I was like that. But all I know is that, I love the feeling of having many guys admiring me for me being easy to get along with because I am too friendly and I do favors for them whatever it is they wish for me to do just for the fun experience.
"Mischieve with erotic romance exploring and expliciting sexual fantasies for the purpose of pleasure."
I wasn't a killjoy. But I wasn't too giving also. I just go with the flow, wherever it may lead us to. But of course I was careful. I don't want anyone to know what I was doing. So all my teen age life, was full of lies, just to get what I want, and to be with whom I want to be with. And I was good in that. I am the "best secret keeper" plus I am the best "block mailer" who give threats to protect myself. I manipulated those guys I were with that time and they never tell anyone what we did. They were never the type of guys who loves to kiss and tell. Because they are afraid of what I was capable of doing. Because when someone betray me. For sure, I'll get even.
And so I turned being "playful" and cruel. I urged for more flesh of men like a "nympho". Not just ONE, not just TWO or THREE, but I wanted more! I do not know why I was like that. But when guys tempt me, I easily give in. I'm not saying I am easy to get. But honestly, I just don't think at all. With my faith falling once again because it was built without strong foundation", I guess...
One night at a bar, I danced with my girlfriends all night long. We met a lot of different men, and of course! What do you think, usually happens after a party? You get drunk and wasted. But I didn't lost myself even if I was too drunk that time. Maybe because the effect of the alcohol in me isn't the feeling of throwing up and feeling dizzy, but the effect of the alcohol in me is being horny. I was so "drunk", and because I was drunk and I easily trust people. I didn't knew that the guy I flirted with putted some drug in my liquor. That made me, Oh! so feeling high and sexy. So we've done it. We played like porn stars for a party of 4 including the little guy with a video camera recording the scene. I still know what was happening. I tried to FIGHT them.
"Obscene and human bodies used by demon like beings"
One is okay with me but not more than one during that time. I tried kicking and pushing and screaming but then no one heard me, they were 4. One who was covering my mouth, another one who was holding my hands high while laying down in bed, one holding my feet so i wouldn't be able to kick and THE other one fucking me. Then after him comes another one, and another one and another one. And the actual guy who I were with at the bar, went back inside and told me not to tell anyone because we were all UNDER the influence of "ecstacy" and no one will believe me. And so i just kept quiet. I can never forget that moment. For what I felt during time was mixed pain and pleasure. And swear, it will never happen again. I buried that particular event in my life on the GROUND and started a new life again....trying to cope up the burdens in me WITH music in my life instead of PRAYERS My faith got lost again.
There comes MY SECOND CHANCE AGAIN.


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It only takes a minute to get started. Fill out our uncomplicated sign up form and login to the site.
This step is discretionary but will dramatically enhance your chances of getting interest from other Christian members on the site. Let people know a bit about yourself, your hobbies and upload some good photos of yourself.
Use our website's cutting-edge search to locate members who match your requirements. Or just flip through listings of Christian singles and locate members that you find interesting.
Identified someone who you're attracted to? Send them a message or just a wink and await their reply. With a little luck you'll be on your way to discovering that special someone for you.
You won't regret it


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Heat Is Overrated.
When Jaquel Moroz meets the most frustrating young man in the universe, a strange duty gets passed onto her.
Or: The one where Jack Frost is possibly the most confounding/irritating person on the planet, and decides he's had enough. He then proceeds to go on a lavish vacation in the Bahamas. The result? The exotic islands get oddly colder in a bizarre case of climate change, and Jaquel Moroz becomes Jaq Frost.
AKA: I was bored.
Marlene hummed. Her lips curved in a wicked smile, and she sauntered over to her sister's bedroom. Knocking on the door, her tanned knuckles connecting with the wood, she barely waited for a reply before pushing the door open and entering.
"Yes, Marl?" Her sister asked, distractedly, though a slight tone of amusement tainted it.
"Well, sister dear, there's this boy, and he's really nic-"
She pouted. "Why?"
Jaquel leveled her gaze to her sulking sibling. "Because I am not going to drive you to some strange boy's house while the nanny's away."
Marlene narrowed her eyes. "Bertha won't mind," She argued.
"Yes, she will. She threw a fit over Tony, remember?" Jaquel cocked an eyebrow pointedly.
"Hmmph. Fine." The girl stormed out and went to stew in her own room.
Jaquel sighed and went back to her work.


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Ascites is defined as collection of excess free fluid in the peritoneal cavity and it could be due to a number of different medical conditions. A proper history and clinical examination may reveal the underlying cause but still certain general and specific investigation are to be carried out in order to confirm or find out the possible etiology.
General Investigations
1. Urine Dipstick: It is a simple and cheap procedure that will be strongly positive for protein in nephrotic syndrome. If so, a 24 hour urine collection for protein should be undertaken; more than 3.5gm is indicative of nephrotic syndrome.
2. Complete Blood Count: Raised white cell count may indicate an infective etiology but a differential white count is more specific.
3. Urea and Electrolytes: Elevated urea and creatinine may indicate a renal etiology; however , it may also be a component of hepatorenal syndrome, which is renal impairment secondary to liver failure.
4. LFT's : May be deranged in the presence of liver disease. The serum albumin will be able to indicate hypoalbuminaemia but the underlying cause must still be sought.
5. Chest X-ray: Findings suggestive of cardiac failure are cardiomegaly, upper venous diversion of blood, the presence of Kerley B lines, pulmonary edema and pleural effusion. Occasionally the presence of carcinoma may be suggested by a mass in the lung.
6. Ultrasound Abdomen: In addition to confirming the presence of ascites ultrasound will detect any intra abdominal masses that are not palpable on clinical examination. It may also indicate the presence of fatty deposits in the liver in the presence of cirrhosis. Dilated collateral veins may be visualized in conditions that cause obstruction of the venous outflow of the liver, including cirrhosis and Budd-Chiari syndrome.
7. Abdominal Paracentesis: Aspiration of the ascitic fluid is very useful to help determine the underlying cause. A sample should be sent to microbiology clinical chemistry and pathology.
Ascitic fluid Examinations
Chylous The milky white appearance of chylous ascites is due to obstruction of the lymphatic ducts. Continue reading "Investigations Required In Patients With Ascites"


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What is dissociation? Why does it occur? How does it manifest? When does it lead to a dissociative disorder? What are depersonalisation and derealisation? Are dissociative disorders for real? How do you support someone who suffers from trauma-related dissociation? How do you recover? What is it like to be dissociative? Dissociation is a normal response to trauma, and this course will cover a range of issues fundamental to understanding this phenomenon and how it presents in trauma survivors' lives. It is suitable for a variety of professionals working in this field, along with survivors themselves. For more information see the DID and Dissociation: The Fundamentals overview page.
Please note lunch WILL be provided on this day. Please provide details of any allergies or dietary requirements (including if vegetarian) in the comments box during the booking process.
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|Saturday 10 June 2017
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The RED Rod To Rosette Bracket is a bracket that can be mounted to any 19mm rail. This also mates with RED rosette-based accessories such a Dogbone A [Straight], Dogbone B [Jogged], and Dogbone C [Arched].
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By Viktor Hanacek
Collection Spring Flowers
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May 18, 2017 / Viktor Hanacek
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picjumbo is free stock photo site created by designer & photographer Viktor Hanacek in 2013. It all started when any regular stock photo site didn't want his photos due to lack of quality. Two years later people downloaded more than two and half millions images from this site.
Made with love. ♥ 2017 picjumbo, a project by Viktor Hanacek.


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Here is what I love about this product:
1. Two taps of the brush are enough to get product sufficient for the whole cheek. It is insanely pigmented, but the pigment is not too bright.
2. The color blends out gorgeously and smoothly and is a completely natural-looking flush that can blend with either cool-toned or warm-toned makeup. It leans cool when I wear it with cool shadows and lippies, and warm when I pair it with bronzes and golds.
3. It looks five hundred percent natural, even though I committed the cardinal sin of using a blush not specifically targeted to my skin tone.
4. It wears beautifully and lasts all day.
5. It is creamy and blendable and not at all sticky, tacky, gloppy, patchy, or any of the other things people don't like about cream blush.
Here is what I don't love about this product:
Seriously, I cannot recommend this product highly enough. The only problem with it is that you don't get so much for the money: it's $22 for 3.5 g. However, there are other products both better and worse than this: Stila CCs are $25 for 4.25 g, but Becca creme blushes are $30 for 3 g.
The next time she has a sale, I'll definitely be picking up the "Rosy" shade; the "Toffee" looks quite a bit too dark for me but would be gorgeous on darker-skinned women.
From - http://www.foxyvoxy.com/2011/01/05/review-eve-pearl-hd-foundation-cream-blush-in-ruby/


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Protect Your Teeth Over the Holiday Season
posted on
'Tis the season for family gatherings, special events and many meals around the holiday dinner table. It's also the time of year when overindulging in sweet treats and desserts can become part of the daily diet, and though that occasional splurge won't do much harm to your diet regiment, eating and drinking sugar-laden treats all season long can take its toll on your teeth.
You and your family can enjoy a safe and happy holiday season by protecting your teeth and keeping sugar intake at a minimum.
Here are some ways to protect teeth over the holiday season:
1. Avoid the grazing habit. It's easy to snack on holiday cookies, sweets and candies throughout the day, especially if you're spending time at home and just relaxing. Avoid keeping these sweet treats within arm's reach, and consciously decide to bring them out only for special events or when guests are visiting. Your teeth – and waistline – will thank you.
2. Enjoy some sugar-free candy. There are several sugar-free candy alternatives to the usual lineup of holiday candies, and you can enjoy a piece of candy once or twice per day if it's a sugar-free version of your favorite. A low-sugar diet can also prevent the sugar high-and-crash cycle that often trigger rapid mood swings and anxiety. Keep sugar-free candy on hand to keep everyone's mood stable – and preserve those teeth in the process.
3. Squeeze a dental cleaning session into the holiday schedule. Get yourself and the kids on a healthy track for the holidays by booking a dental appointment for teeth cleaning with a dentistry doctor. Keeping the teeth clean and healthy and learning about any cavities or potential tooth problems can get everyone motivated to eat healthy foods and keep their teeth in great shape when temptation strikes over holiday season.
4. Give away extra Halloween candy. Sugar is the culprit behind tooth decay, corroding tooth enamel and even gum problems. If you have excess Halloween candy on your hands, consider donating it to a food pantry or local charity so your household doesn't end up consuming the extra sugar this season.
5. Keep up with a consistent blush-and-floss routine. Brushing your teeth at least twice per day and flossing after each session can keep excess sugar off the teeth, prevent tooth decay and stop plaque buildup. Keep up with your healthy routine so that your mouth is fresh and clean – even if your diet isn't up to par. If you don't know how to floss correctly, ask your dentist about tips on flossing and products that can help you get started.
Learn more about dentistry and cosmetic dentistry procedures and treatments available in your area by reviewing our information guide, or consult with a general or cosmetic dentistry doctor in your area for a consultation and treatment plan that will take care of your teeth.


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Sorbus Aucuparia otherwise known as mountain ash or rowan is deciduous, with a rounded growth habit. It carries greyish, downy winter buds that open to dark green leaves, turning red or yellow in autumn. From April to May it produces clusters of white flowers which are then followed by orange-red berries in June. Sorbus Aucuparia is ideal for autumn colour, and for attracting wildlife. It can grow in most well drained soil and will tolerate partial shade, although there will be a more prolific show of flowers and fruit if the plant is exposed to full sun. Supplied in a 15L.
Important Features of Sorbus Aucuparia:
Height: 250-300cm
Pack size: 1
Foliage colour: Green
Supplied in a 15L pot
Click the More Info Tab for Further Details
More Info
|Ultimate Height
|Ultimate Spread
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Collidemovement.org is a 1 year old domain, situated in Viet Nam. The domain is linked to the IP address 188.8.131.52 which is provided by the hosting company SUPERDATA.
It was registered by Cong ty Co phan Dao tao AIMACADEMY , who can be reached at . The site is owned by Cong ty Co phan Dao tao AIMACADEMY. Registration details show that it was registered on 08 Jan 2016 through p.a. viet nam company limited and will expire on 08 Jan 2017.


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The Russian connection: See all our coverage | Read about our investigation
The media framing of today's national security speeches by Barack Obama and Dick Cheney as a sort of "showdown at noon" has struck me as pretty bizarre. And yet....I just read both speeches and I have to admit that it's really not so bizarre after all: they could hardly form a starker contrast if they tried. Obama's speech is all about the rule of law, honoring American values, creating policies that look beyond just today and tomorrow, and trying to figure out how to gain genuine security in a dangerous and complicated world. Conservatives are going to absolutely howl over it.
And then there's Cheney: no regrets, no second thoughts, not even an admission that any kind of balance should be entertained ("In the fight against terrorism...half-measures keep you half exposed"). It's a pure, white hot defense of an absolutist military approach to every aspect of national security. Among liberals, Cheney's reputation as a panic-stricken Buck Turgidson will be confirmed beyond doubt.
I want to read both of the speeches before I say any more. But really, the contrast is truly spectacular. It's worth your time to read them if you haven't already.
UPDATE: David Corn has a good summary here. Jacob Heilbrunn has a good take here.


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Hollywood stars including Leonardo DiCaprio are set to launch Macau's newest $3.2 billion casino and entertainment resort, as the southern Chinese enclave's high-roller gambling business takes a hit from China's corruption crackdown.
A day before the official launch of Studio City, developers of what is billed as an "integrated resort" said Monday they are bullish about the future despite the slumping gaming revenues.
Macau in September saw its 16th consecutive month of declining gaming revenue, 33 percent lower than the same month last year, as Beijing encourages the territory to diversify from gambling and reins in high-rolling visitors from the Chinese mainland.
Gross revenue for September was 17.13 billion patacas ($2.15 billion), according to figures from the former Portuguese colony's Gaming Inspection and Coordination Bureau.


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I stumbled upon this video of an 84 year old grandfather playing Call of Duty and Halo with his grandsons. It is cute, hilarious, and awesome. Check it out!
I stumbled upon this video of an 84 year old grandfather playing Call of Duty and Halo with his grandsons. It is cute, hilarious, and awesome. Check it out!
Ever get tired of eating those boring old dots and healthy old fruits. Well here is a picture of all kinds of alternate Pac-Man ideas.
I know I'm a little late on this but here is one of the official trailers for the new Superman flick . This one features the voice of Kevin Costner who is playing Clark Kent's adoptive father in the movie. There is an alternate version as well with all the same footage but has the voice of Russell Crowe who is playing Jor-El Superman's biological father. Both are giving a bit of fatherly wisdom.
This clip doesn't show much but I really think this movie is going to be far better than any of the other Superman movies that we've seen. Superman has always been a little to apple pie for me and it looks like they've gone a more gritty and dark route this go around….and oh Henry Cavill (Superman) is SMOKIN HOT to any of the lady fans out there. What do you all think?
Now if you're a tagger this is how you do it right here. Check out this Star Wars Graffiti Art that adorns walls, tunnels, alleys and a number of other urban surfaces throughout the world.
Japanese artist Nagai Hideyuki is able to draw 2D images that present the illusion of being 3D through the use of two sketchbooks and some creative angles. For even more of Hideyuki's work check out his website, Facebook Page, and deviantART Page. Enjoy!
Who wants to go see The Dark Knight Rises in style? Well today is your lucky day. Check out these Batman inspired threads and accessories.
Want to look like Bruce Wayne. No problem, just rock some Giorgio Armani.
Bat Symbol Money Clips
Batman Kicks
Batman Watch
Batman Button Downs
Batman Adult Backpack
Batman Shades
Sons of Anarchy is another one of those shows I got unexpectedly sucked into. I haven't heard much about Season 5, however, I came across this little tidbit. So if there are any other SAMCRO fans out there I hope you enjoy.
Art truly knows know boundaries. You show me something that you think can't be turned into art and I'll find an artist to prove you wrong. Check out these manhole covers and sewer drains that various artists took it upon themselves to turn into works of fine art.
Hyundai has done it. They have created a Zombie Survival Car in the form of an apocalyptic souped up Elantra Coupe to celebrate the 100th issue of The Walking Dead comic. It comes complete with front spikes, wheel spikes, barred windows, and flood lights. Check it out! Also scroll to the bottom for a 5 minute video on how this beast was made.


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Escorted by Gregory (Part 2)Mature
Despite her reservations, Sarah accepted the goblet that Gregory graciously offered to her upon his return. One sip confirmed her suspicion that he was trying to make her pliable by intoxication. Too bad he didn't know that she was used to drinking with the boys. Sarah smiled as flattery began to roll off his tongue.
How different and yet similar things were here. Though a quarter of the things Gregory said barely made sense to her, he was still a player. Sarah laughed and shook her head as his latest comment made no sense at all.
"You do not think your eyes are like freshly plowed wheat fields?" Gregory asked, his face frowning in confusion.
Sarah eyed him. "I have plowed wheat fields, and I don't think my eyes are that shade of brown," she chuckled.
"You have plowed fields?" Gregory immediately took her hands in his own and examined them.
"And other women obviously eat up this crap that you feed them?" Sarah asked pulling back her hands.
"Crap?" he asked, clearly startled. "I only but tell the truth in the most poetic form I can." He scooted closer, standing as she did.
"Do you?" Sarah asked looking into his eyes. "Perhaps you feel you are, but why," she leaned forward placing her hand between his face and hers, promise ring glinting in the sun, "would you bother with such poetry upon me, your brother's fiancée?"
"I would not think there is much hope of your Tri-Luna lasting," he stated. "Presuming, of course, the betrothal even makes it through the ceremony." He reached up and clasping her hand, kissed it.
"Really?" Sarah was indignant. "Do you really think so little of your brother?"
"I only know what happened last time." Gregory moved her hand from between their faces. "The Gods have some lofty plan for him that would only leave you in heartache."
"Is that so?" Sarah breathed deep to steady herself.
"Yes," Gregory's eyes flicked to her cleavage before seeking out her eyes. "After all what is the promise of engagement, but prelude to the torturous denial of pleasure during Tri-Luna. Surely one moment is but naught in the eternity you will spend with my brother."
"You're kidding, right?" Sarah stepped back only to find herself gently restrained by a hand in the small of her back. Shaking her head with a snort, she pulled her hand from his.
"Just a kiss, surely it can't hurt. Perhaps you might see..."
Sarah didn't wait for him to finish. Turning she grabbed the hand from her back and sent Gregory to the ground. She knelt beside him, pressing his arm into his chest.
"Do not think you can take advantage of me, for Jay and I go back a long ways. He gave me my ring and I know he intends to follow through with it even if our marriage turns us to paupers."
Letting him go in disgust Sarah stood and began to brush off her skirts. Echoing against the house, three slow claps caused her to look up. Lord Robert stood at the door to the garden.
"Father, I..." Gregory began.
"Enough," Lord Robert interrupted him. "Be glad the Gods did not see fit to have you marry her for I think it would do you good to be hen pecked."
Sarah glanced at Gregory. "I don't think it would work." She shook her head. "Besides,' she smiled at Lord Robert, "I love Jason."
"Yes well," Lord Robert snorted, "I hope Gregory hasn't disorganized your thoughts. I would like to know why you think you are worthy to be considered suitable for my son." He turned and bellowed into the house, "I want the family in the Sunroom now!"
"I told you not to try anything on her," Mathew chuckled.
Sarah smiled sweetly at the smirking Mathew. "Well, I wouldn't want you to feel lonely in the rejected department." Smothering her mirth at Mathew's glare, she tried her best to glide into the house.


text:
Using new private benefit exchanges, employers can bring the attractions of consumer-centric online shopping to benefits, giving employees unprecedented flexibility in benefits choices -- while also controlling costs through a defined compensation approach, just as they've done with retirement funding.
In this book, private benefit exchange innovator Alan Cohen introduces the concept to consultants, HR professionals, business owners, and insurers, and presents complete best practices for successful implementation. Drawing on his experience pioneering private benefit exchanges with Liazon's Bright Choices, Cohen:
- Shows how online shopping has transformed consumer behavior, and how to apply these insights to the benefits industry
- Explores the important shift to a defined contribution approach for benefits: the next wave from fee-for-service to managed care, to consumer-driven high-deductible health plans with HSAs
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- Explains the role and value of brokers in the marketplace
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- Projects the impact of trends such as industry consolidation, the Affordable Care Act, and its public exchanges
- Examines the expansion and progress of the exchange system via multiple case studies
- Shows how to achieve the full potential of choice marketplaces


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New Braunfels Herald Zeitung (Newspaper) - October 23, 2003, New Braunfels, Texas Thursday, October 23, 2003 — Herald-Zeitung — Page 3A MEDICARE & MEDICAID NOW PAY for Chicote Meters & Suppiiet for Insulin Si Non insulin Dependent Diabetic Patients For over eight >earv. we have had a Diabetic Program that has helped thousands of texans obtain equipment and supplies needed for Blood Glucose testing Medicare pays 80% of the charges lot (ducosc Meters and Supplies, most co-insurance and Medicaid pays the 20% balance hor More Information, Please Call or Come By Professional Medical 608-9577 H www proteaatonaimodicAJ com 386 A [.anda • New Braunfels, lexas_ FREE >ELIVERY. JOE H. ISBELL Funeral arrangements are pending at Doeppenschmidt Funeral Home in New Braunfels for Joe H. Isbell, age 80, who died Wednesday October 22,2003 at his home in New Braunfels. Doeppenschmidt Funeral Home REV. ELMER H. GUMPER A memorial service for Rev. Elmer H. Gumper will be held Saturday, October 25, 2003 at ll a.m. at First Protestant Church in New Braunfels. There will be a pre-service "Celebration of Life with Music" beginning at 10:30. Memorial contributions may be made to I lope Hospice, Eden Home, Inc., First Protestant Church, Faith United Church of Christ, Community Service Center or Slumber Falls Camp or to the charity of the donor's choice. NBISD CONTINUED FROM Page 1A Board discussing bond payments Texas Education Agency recommends districts maintain a fund balance of 12 to 20 percent of operating costs, said Hill. There is no minimum required fund balance. The current school budget has a $10.3 million fund balance, which is 28.6 percent of the operating budget. "Over the last five or six years, it's been growing," Hill said. In 1998 the fund balance was at $3.3 million. "The fund balance many years ago got down to a dangerous level," said Ijee Edwards, NBISD board president. In the past, the school district drained the fund for budget deficits and other expenditures, he said. This year the fund balance will pay for the current $1.2 million budget deficit. "We hope by the end of the year to reduce expenditures so we don't have a deficit," Hill said. Last year, NBISD started the fiscal year with a $200,000 deficit and ended up with a $1.5 million fund balance. No one expected that kind of savings and the district shouldn't expect that this year, Edwards said. "With the volatility of the Legislature and funding being in limbo, it's very important the school district be able to meet possible catastrophe for the failure of the state to fully fund its prior obligations,'' Edwards said. A strong fund balance could help earn the district a better bond rating, reducing payments and taxes. But the increase is the district's "safety net," he said. "We have to plan on less .than status quo," Edwards said. "If you're smart, you have to bet on catastrophe." In 2001, the district estab lished a savings plan for depreciating capital. Still, NBISD must buy buses for the next few years to upgrade its fleet, Edwards said. The fund balance is not used for recurring costs like personnel or the regular replacement of vehicles. "You don't use fund balance for salaries," Edwards said. "You don't use fund balance for equipment that you buy every single year." Because the district has accumulated a substantial amount of fund balance, the board has discussed making bond payments. Edwards said when the fund balance reaches 33 percent of the operating budget, the board should consider lowering the tax rate or spending down the balance. "That means that we've been too conservative on our approach and we should go ahead and Hind some things that we may have been (shorting)," Edwards said. This year's negative budget encouraged the board to consider hiring a consultant to take an objective look at district spending. A workshop is slated for Nov. 24 for the newly elected board to hire one. By Dylan Jim6nez Staff Writer Ann Rogers was diagnosed with breast cancer 25 years ago. Her doctor asked her to start a support group. "I told him, 'I don't want to talk about cancer. I want to forget about it,'" Rogers said. IYvo years later she visited her first breast cancer patient, and she has never turned her back on a fellow cancer patient. Rogers recently hosted the fifth annual luncheon for a group of breast cancer survivors. Women still in treatment and 20-plus-year survivors meet monthly. Once a year they have a lunch to tdk about life after treatment and continuing treatment. They talk about family and common friends and about their disease. LORA CONTINUED FROM Page 1A Building gc historic tax d ets reak Fredericksburg Road. It is the one of several sites Peel searched for artifacts that revealed anything — and the only site that will be affected by construction, he said. "We've found a few arrowheads, but nothing major at this point," Peel said. A team of archaeologists would completely excavate the area, the developer added, because it will be covered with an access road when the project is built out. Peel said the site had received the historic designation that will qualify the project for a preservation tax credit. "We're meeting with the state next week down there to go over window issues," Peel said. Happy Belated 1st Birthday Zachary Mathieo Love. Your Mom. Sister' Alyssa. Grandma & Aunt Christi LOUDY, LORDY OUR DADDY IS 40! Love, Andres, Araceli, Carlos & Cesar Your like fine wine, yon age better with time! Love Yvette HEAR WELL AGAIN "Home of America's most FREE HEARING CHECK UPS THIS WEEK EF ST OFFICE 830-997-8515 www.fbgfoodandwinelest.com Critical responsibility.Area cancer survivors celebrate life Households using medically essential electr leal equipment may qualify as "critical load accounts Contact any PEC office to icceive paperwoik you! physician can complete During a set vice interruption we try to qtve pnoiity to these members when lestonng electricity but piompt payment of electiic bills is still required Make; that cntica! call today PEDERNALES ELECTRIC J) W /oui Power Pi of(%sioi H ii J OSK'4 • 8 8 8*5 5 4 - 4/ 3 2 HL-Ut Marktplatz Downtown Fredericksburg October 25, 2003 ADMISSION: gates open at noon Adults • $20 (under 21 • $5) Under 12 free • Entertainment • Texas Wines and Foods • Games Auction • Cooking School plus 3 Special Events (Tickets sold in advance only, call please Doute Wills, Hewing Aid Speiiaiiit, helping citizen* oj New Braunfels hear benet far more than 20 years NIWMAUNMU: 532 N. Union Call for an appointment 830*620-4327 DID YOU ImlU w mf I ■ 267,000 women will be diagnosed with breast cancer in 2003. ■ 39,800 women will die from breast cancer this year. ■ 1,300 men will be diagnosed with breast cancer in 2003, and 400 men will die. "We come to celebrate life," said Becky Voges, who organized the group out of a small number of survivors from St. Paul's Lutheran Church. "There wasn't one [support group] in our community," said Voges, a five-year survivor. "I was looking for help and for hope." The women in the group try to support the newly diagnosed, Voges said. "That's what helped me, meeting 20-year survivors," she said. "I didn't know there Peel said he was seeking state approval of construction details that include the type of glass that would be used in the project and how it would be fitted so that from outside the building's windows would not reveal interior partitions. Peel said he was tentatively scheduled to close on purchase of the property in December depending upon state approval of his working drawings. "As a practical matter, nothing goes as you think it's going to go, so I suspect we're proba-bly looking to February or March, something like that. The work will start everywhere. We intend to file for a build- Dunn's MI I AR 72108 Tmh* fish fa rm.com Channel Catfish, Bass. Hybrid Bluegill. Fathead Minnows. Koi, Crappie. Coppernose. Redear Bream and Triploid Grass Carp are now available for Pond & Lake Stocking. Permit and one week notice required for Triploid Grass Carp purchase • We furnish hauling containers! • Live Delivery Guaranteed! • Discounts/Special Deliveries on large orders! • Turtle Traps. Fish Feeders. Fish Traps • Liquid Pond Fertilizer and Gift Certificates Delivery will be WEDNESDAY OCT. 29. 2003 at the limes listed for the following towns and locations. Mason-Donop Feed Store 347-6371 8-9am Fredericksburg-Farmers Grain Company 997-7561 10-1 lam Kerrville-Farmers Grain Company. Inc. 257-6355 12-lpm Boeme-Barkley's Home & Ranch Center 249-2656 2-3pm New Braunfels-Producers Cooperative 625-2381 4-5pm To place an order or for more information, call one of our Aquatic Consultants, local dealer, or email wk^iiunnillsMitrinii&m Offices located in Oklahoma & Arkansas! M-F 7am-8pm Sat 8am-6pm CST !-80(M33-2950jFaxl-870-58'^0^^vw^JunnsfishfanTG»om^^^y popular digital hearing aids." REBECCA S. ROGERS/Herald-Zeitung Breast-cancer survivors Terry Burrio, left, and Ann Rogers visit with with members of a survivors' support group Monday. was such a thing." The survivors show those going through chemotherapy there is a full life beyond treatment, she said. More than 70 women have participated in the group over the years, many coming and ing permit in the next IO days." Peel said a lot of planning had been completed. "We've done all the construction drawings, the garden is all designed, the air conditioning and everything's going as they need the support, Voges said. Women as young as 16 and as old as 90 have found support and camaraderie. "This is not a downers group," White said. "It's cheerful." designed," Peel said. To make the apartments fit around four 120-foot-high atriums required 38 different designs — for 108 apartments of sizes ranging between 950 and 1,000 square feet. ;
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Insurers have a critical role to play in helping to support the transition to a low carbon world through sustainable investments and through insuring renewable energy and energy efficient homes, said Matt Cullen, head of strategy at the Association of British Insurers (ABI).
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Association of British Insurers, Matt Cullen, European Commission, Insurance Europe, Europe


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Too bad it was more than a pie and a heckler in the back.
Huma probably threatened to turn him over to her mother. You know the Mom who is a member of the female arm of that peace-loving humanitarian group, the Muslim Brotherhood. No doubt they could come up with some "therapy" to cure him.
Ms. Abedin had no sense of Huma about this non-halal Weiner.
Score one for a Muslim as the "good guy" (it had to happen sooner or later, although now we return to our regularly scheduled jihad).
Weiner was a gaping below-the-waterline hole in the 'rat partyboat.
I was hoping he'd stay on and maximize the damage to the Vermin Party.
"Looks like we won't have Weiner to kick around any more."
Nope; after he resigns, I don't believe he will ever be heard from again. From what I understand, he never bothered to acquire any marketable skills other than "politician." How long until Huma tires of him working the late shift stocking shelves at the mini-mart?
"Weiner to QUIT after showdown talks with wife"
Huma, "Anthony, I can't pretend to be a straight woman if you sext the world while married to me! How can I pretend to be hetero if you won't show the pretense of being my husband?! Hillary will have your b-"
Our intrepid reporter ran away at this point because a lockbox came flying out of nowhere..
I wonder what kind of punishment would be given to Weiner in Huma's culture? Does she have brothers or uncles? He better watch his back...or should I say "his front" ???
The people in his district that voted for him, term after term, deserve the little weasel wiener. Very disturbing that there are that many numb skulls concentrated in an area.
I don't know about kicking around, but for sure we will not have this Weiner to play with!
Why does the ruling class alway say, "I'm gonna resign" instead of just RESIGNING? There's always this prelude to the event.
Weiner splits in the heat, eh?
True that. Call me a sceptic but I question the photo of Huma driving herself in such a delicate state of pregnancy with all the media out to get her photos. Don't you think she would have been escorted home, especially by a request from the Clinton's? Don't know if I'm buying the pregnancy thing.
Dems to Weiner: "Put down the pump and step away from the Capitol."
Muslim Huma Abedin is saying "I divorce you" three times to Jewish Weiner. A bit of a turn around in the rules of the Holy Koran.
I think they are underestimating him - 'further embarrassment' is coming...
"Maybe I can sleep at Barney Frank's house until she cools off."
I used to think Wiener was crazy ... but now I can see his nuts.
Don't worry about the little wiener, you can bet his comrades have given him something 'special' in exchange for his quiting.
It's the democrat way
Does resigning still leave him vested and eligible for his government retirement pay?
If so, he gets the last laugh, as we'll be supporting him for the rest of his miserable life...


text: Lions' Royster eager for one final season By Joe Juliano Inquirer Staff Writer Evan Royster stood on the field at Beaver Stadium recently and looked around at the empty seats, imagining the time when they would be filled with more than 100,000 fans sending out waves of noise crashing down to the lush green grass. It's exciting for Penn State players to experience that atmosphere, dubbed "The Great Show" by the locals. And when Royster thought about taking in the show for a fifth season, he embraced it while delaying the start of his NFL career. "Just being in the stadium right now makes you think about it," the Nittany Lions' star running back said earlier this month at the team's media day in State College. Many observers say there is a good reason why Royster came back. The Fairfax, Va., native is just 481 yards away from breaking the Penn State record for career rushing yards held by Curt Warner, who amassed 3,398 from 1979 through 1982. There's more, however. To hear Royster tell it, it's "the stadium, the atmosphere, everything that comes with college." "There wasn't one specific reason that was the deciding factor," he said. "It was a lot of things. I wanted to have my senior year here. I wanted to come back and be able to improve my draft stock. I wanted to have another shot at a Big Ten championship. It's a lot of things. "I don't think the money was worth it. College is an experience. It's been fun so far, and I'm hoping to get another year like" the previous ones. Royster, who redshirted as a freshman, announced he would return for his senior season less than one week after the Nittany Lions ended their 2009 campaign with a Capital One Bowl win over LSU. NFL draft observers had projected Royster as a mid-round pick. Read more: http://www.philly.com/inquirer/spor...eager_for_one_final_season.html#ixzz0yHwl2ain Watch sports videos you won't find anywhere else


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Life After Death
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Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye
Applied microbiology
The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus.
The detection limit of LAMP using
The LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test.
Human astroviruses (HAstV) have been shown in several epidemiologic outpatient studies to be an important cause of viral gastroenteritis in infants and young children. HAstV have been associated with outbreaks in day-care centers for children and adults
Astroviruses are among the most resistant viruses; they show resistance against different physical and chemical agents, they are able to maintain their infectivity at 60°C for 10 min, and they are resistant to treatment at pH 3
Several detection methods have been developed to detect the presence of astrovirus in clinical isolates, raw sewage samples, groundwater and surface water, including cell culture
To meet these requirements, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction was developed as an alternative method. The LAMP assay is a rapid, accurate and cost-effective diagnostic method that amplifies the target nucleic acid under isothermal conditions, usually between 60°C and 65°C
The LAMP reaction results in large amounts of pyrophosphate ion byproduct. These ions react with Mg2+ ions to form the insoluble product, magnesium pyrophosphate. Because the Mg2+ ion concentration decreases as the LAMP reaction progresses, the LAMP reaction can be quantified by measuring the Mg2+ ion concentration in the reaction solution
HAstV is classified into eight distinct antigenic serotypes, HAstV 1–8, with serotype 1 predominating in most countries
Optimized LAMP reaction
The LAMP reaction was performed using plasmid DNA as template to determine the optimal reaction conditions. The optimal concentrations of betaine and Mg2+ ion in the LAMP reactions were 1 mmol·L-1 and 4 mmol·L-1, respectively (data not shown). The amplicon was formed at 63, 64, 65 and 66°C, with the optimal temperature for product detection being 65°C. Thus, 65°C was used as the optimum temperature for the following assays. Although we could detect well-formed bands at 60 min, the reaction time was extended to 90 min to ensure positive detection of lower concentration templates in the system.
Naked-eye observation of LAMP products using HNB
The LAMP reaction was incubated in a conventional water bath at 65°C for 90 min, followed by heating at 80°C for 2 min to terminate the reaction. The ability to detect astrovirus LAMP products using HNB was examined. Positive amplification was indicated by a color change from violet to sky blue, as shown in Figure
Figure 1
Detection of LAMP products by observation of white turbidity and the color of the reaction mixture
Detection of LAMP products by observation of white turbidity and the color of the reaction mixture. (A) LAMP detection of astrovirus by electrophoresis; (B) Color reaction with HNB; (C) White precipitates M: Marker; CK: Blank control; S: Astrovirus.
Specificity and sensitivity of the LAMP assay
The sizes of the LAMP fragments digested with the restriction enzyme, EcoN1, were analyzed by electrophoresis, and the results showed agreement with the predicted sizes of 84 and 135 bp (Figure
Figure 2
Specificity of astrovirus detection using the LAMP assay
Specificity of astrovirus detection using the LAMP assay. (A) Restriction analysis; (B) Specificity analysis of cross-reaction by electrophoresis M: Marker; CK: Blank control; S: LAMP products after digestion with EcoNI 1: Astrovirus; 2: Rotavirus; 3: Norovirus.
The reaction was tested using 5 μL of 10-fold serial dilutions of
Figure 3
Sensitivity analysis of LAMP detection of astrovirus
Sensitivity analysis of LAMP detection of astrovirus. (A) Electrophoresis; (B) Color reaction with HNB M: marker; CK: Blank control; 1: Astrovirus RNA 3.6 × 109 copies·μL-1; 2: 3.6 × 108 copies·μL-1; 3: 3.6 × 107 copies·μL-1; 4: 3.6 × 106 copies·μL-1; 5: 3.6 × 105 copies·μL-1; 6: 3.6 × 104 copies·μL-1; 7: 3.6 × 103 copies·μL-1; 8: 3.6 × 102 copies·μL-1; 9: 3.6 × 10 copies·μL-1; 10: 3.6 copies·μL-1; 11: 3.6 × 10-1 copies·μL-1.
Evaluation of RT-LAMP assay with reclaimed water samples
Comparative evaluation of RT-LAMP with routine RT-PCR was performed to examine astrovirus in 12 reclaimed water samples. Five samples (No. 2, 3, 4, 6, 9) were positive and the frequency of astrovirus detection was 41.7% (5/12) with RT-LAMP (Figure
Figure 4
LAMP for detection of astrovirus in water samples
LAMP for detection of astrovirus in water samples. (A) Electrophoresis (B) Color reaction with HNB M: Marker; CK: Blank control; S: Astrovirus; 1-12: Samples.
This study demonstrated that the LAMP method described here for astrovirus detection is highly sensitive, and can detect as few as 3.6× 10 copies·μL-1 of astrovirus template RNA. The detection limit of the RT-LAMP assay with HNB for pandemic influenza A H1N1 virus was approximately 60 copies in a 25 μL reaction mixture
Though DNA plasmid is served as template for optimizing virus detection assays in some cases
In this study, we only compared the specificity of the reaction for astrovirus, rotavirus and norovirus because the reported frequencies of infection by rotavirus, astrovirus and norovirus are 59%, 8% and 6%, respectively, in Beijing
The use of HNB for visual inspection of LAMP amplification products was a simple and effective technique, with no gel electrophoresis and staining with ethidium bromide required. Hence, LAMP is a superior method in terms of its economic feasibility and safety. The HNB dye-based assay has a remarkable advantage compared with other color-based assays because (i) opening the reaction tube is not required to determine whether the reaction is positive or negative (this reduces the risk of cross-contamination); (ii) the detection sensitivity is equivalent to that of SYBR green assays; and (iii) the positive/negative result of the LAMP reaction can be easily judged with the naked eye
Using the LAMP assay, we were able to detect astrovirus in various environmental water samples with a simple water bath. A water bath is the only equipment needed, and is used for both the DNA preparation and nucleic acid amplification. With no complicated equipment and technical training, LAMP is very simple to perform and offers advantages compared with other techniques
The LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test.
Design of RT-LAMP primers
A set of four species-specific RT-LAMP primers was designed to target the HAstV-1 capsid protein gene (ORF2), as described by Guo et al.
Figure 5
Oligonucleotide primers used for RT-LAMP of astrovirus
Oligonucleotide primers used for RT-LAMP of astrovirus.
Construction of the pGH plasmid
A recombinant plasmid, pGH-A-X3178G, was constructed as a template for the development of the astrovirus RT-LAMP protocol. A 449 bp astrovirus ORF2 DNA segment (GenBank accession number, GQ169035.1) was amplified and cloned into the pGH vector (AOKE Bio Co. Ltd., Beijing, China) according to the manufacturer's instructions. The DNA segment spanned the sequences between the F3 and B3 primers.
LAMP reaction
The preliminary LAMP for the astrovirus DNA in the plasmid template was carried out in a 25 μl reaction containing 0.2 μmol·L-1 each of F3 and B3, 1.6 μmol·L-1 each of FIP and BIP, l mmol·L-1 dNTPs, l mol·L-1 betaine, 6 mmol·L-1 MgSO4, 2.5 μL 10× Bst-DNA Polymerase Buffer, 8 U Bst DNA polymerase (NEB, Beijing, China) and 5 μL template DNA. The reaction time was optimized by incubating the mixture for 15, 30, 60, 90 and 120 min at 65°C, while the reaction temperature was optimized by incubating the mixture at 60, 61, 62, 63, 64, 65 and 66°C for 60 min. The concentrations of betaine and Mg2+ ion in the LAMP reaction solutions were optimized using a series of concentrations from 1 mol·L-1 to 4 mol·L-1 and from 1 mmol·L-1 to 7 mmol·L-1, respectively. The reaction was terminated by heating at 80°C for 5 min. The LAMP products (5 μL) were electrophoresed on 1.5% agarose gels and stained with GoldView to determine the optimal conditions. The possibility of LAMP detection of astrovirus using HNB (Lemongreen, Shanghai, China) was examined. HNB was dissolved in distilled water at 1.5 mM to prepare a stock solution, and the reaction solution contained a final HNB concentration of 120 μM
PCR detection
PCR amplification of astrovirus DNA in plasmids was performed using the following reaction conditions: 5 μL 10× Ex-Taq buffer, 50 μmol·L-1 dNTPs, 0.12 μmol·L-1 F3, 0.12 μmo ·L-1 B3, 2.5 U Ex-Taq DNA polymerase (TaKaRa, Dalian, Chian), 10 μL template DNA. Amplification conditions were as follows: 94°C, 3 min; 40 cycles of 30 s at 94°C, 30 s at 50°C and 1 min at 72°C; with a final extension of 5 min at 72°C. A positive control and a negative control (nuclease-free water) were included for each PCR assay. The amplified DNA fragments were analyzed by electrophoresis on a 1.5% agarose gel and stained with GoldView. For the sensitivity assay and reclaimed water, 1 μL avian myeloblastosis virus reverse-transcriptase (10 U/μL, Invitrogen, Carlsbad, USA) was added into the reaction mixture.
Specificity of the LAMP assay
The specificity of the assay was tested using DNA from astrovirus and two other enteric viruses as templates, including rotavirus and norovirus. In order to confirm the specificity of the LAMP reaction, the LAMP products were digested with the restriction enzyme, EcoN1 (NEB, Beijing, China), electrophoresed on 1.5% agarose gels and stained with GoldView. Based on theoretical calculations, the sizes of the main bands cut by EcoN1 should be 84 bp and 135 bp.
Sensitivity of the LAMP assay
The detection limits of the rotavirus LAMP assay were evaluated using 10-fold serial dilutions of
Application of RT-LAMP for the detection of astrovirus in reclaimed water samples
Twelve reclaimed water samples previously collected from sewage treatment plants were selected for RT-LAMP analysis. Two-liter samples of surface water were collected in sterile bottles and transferred to the laboratory, where they were immediately stored at 4°C for viral and bacterial investigations. A modified method developed for concentrating viruses in effluent from sewage treatment plants, including reclaimed water, was used to concentrate the water samples
Competing interests
All the authors declared that they have no competing interests.
Authors' contributions
XQH had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis; BYY and XLL performed the collection of water samples and the experiments; XQH wrote the majority of the manuscript; YMW, JQW and YJ supplied technical or material support. ZJW helped to revise the manuscript. All authors read and approved the final manuscript.
This work was supported by Natural Science Foundation of China (51108029), non-profit Industry Financial Program of MWR (201201032), and the Fundamental Research Funds for the Central University (TD2012-03).
Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface water
Identification of viral agents associated with diarrhea in young children during a winter season in Beijing
Human astroviruses in raw sewage samples in Hungary
Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing
Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products
Loop-mediated isothermal amplification of DNA
Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus
Rapid and sensitive detection of Singapore grouper iridovirus by loop-mediated isothermal amplification
Development of a reverse transcription-loop-mediated isothermal amplification assay for detection of pandemic (H1N1) 2009 virus as a novel molecular method for diagnosis of pandemic influenza in resource-limited settings
Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue
A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of human astrovirus
Molecular characterization of astrovirus infection in children with diarrhea in Beijing, 2005–2007
Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater
Detection and distribution of rotavirus in municipal sewage treatment plants (STPs) and surface water in Beijing


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Insurance and reinsurance are highly complex areas in which there is no substitute for market knowledge, technical excellence and sector expertise.
Our Insurance Group is recognised as a leader in the field, bringing a long track record of great results, innovative attitudes, highly effective advice and a level of service few law firms can match.
Our clients include leading UK and international insurers, reinsurers, brokers, third party administrators and financial institutions. They benefit from insurance expertise across our international network which means that we are able to look after their interests wherever they arise.
We are recognised in Legal 500, Chambers, Euromoney Expert Guide and Super Lawyers directories for our insurance expertise, and won the Legal Business "Insurance Team of the Year" award in 2016, after being shortlisted 2011, 2012, 2013 and 2014. Our team also won the Corporate LiveWire 2016 Global Award for Dispute Resolution in the Insurance and Reinsurance Sector, and is shortlisted for the "Legal Firm of the Year" award at the 2016 Reactions London Market Awards.
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Connecting Families to Senior Living
Talk to a local advisor for FREE
Talk to a local Advisor about this community
Contact this community at:
(866) 333-2174
8650 S. Los Feliz Dr.
Facility License: AL1598H
Caretypes Provided: Residential Care Home
Pricing is available for the following rooms:
*Prices quoted are monthly rental charges and are provided by the communities themselves. Actual prices may differ due to one-time fees, timing and care services required. Speak with your Senior Living Advisor to learn more.
Our apologies. Pricing is currently unavailable for this particular community.
Experience Excellence! Our mission is to enhance the physical and emotional quality of being through each stage of our residents' lives. Our impeccable commitment to quality includes respect, compassion, and safeguarding our residents' dignity. We give great attention to detail in our communication with medical personnel. Our employees are of the highest caliber who are carefully screened, supervised, and trained on a continual basis. We have an Open Door policy to encourage family interaction and access 24/7. Peace of mind...yours, your loved one's, and ours.
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This home is the answer to our prayers. It is beautiful, with loving care.
The residents and their families are appreciative of the excellence the owner, manager and caregivers achieve every day.... more...
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Renovated in 2006, this 21-storey city hotel offers guests a choice of 663 rooms and suites, world-class conference and recreational facilities and superb restaurants. Further facilities on offer include an entrance hall with 24-hour reception, a hotel safe, a currency exchange facility and lift access. Guests may also make use of the on site hairdresser, restaurants and public Internet access. The hotel's business centre provides services such as conference arrangements, translations, courier services, library facilities and laptop computers. Audio-visual equipment is also available on request. Room service is available for a small additional fee and there is parking provided for those arriving by car.
The hotel is located in the shopping and entertainment district of Orchard Road, 2 km from Clarke Quay. Buses and taxis are available at the hotel doorstep. Close by guests will find a whole host of shopping centres, including Far East Plaza, Scott's Shopping Centre, Shaw Centre and Tangs Shopping Centre, which also house a number of restaurants, bars and cafes. 10-15 minutes drive away are the Marina Bay Park, Fort Canning Park, Singapore Art Museum, Suntec City Exhibition Centre and the Esplanade Theatres on the Bay. The city centre is just 200 m away. Nightlife at Orchard Towers is just a 10-minute walk from the hotel and there is a metro station (Orchard MRT) 300 m away, giving guests quick and easy access to all the nearby attractions, including the Newton Circus Hawker Centre 750 m away), Fort Canning Park (1.5 km away), Little India (2 km away) Clarke Quay (2.25 km away) and the Esplanade Theatres (3 km away). The hotel is a scenic 25 minutes drive from Changi Airport.
The 663 rooms and suites are located on 2 wings: the Terrace Wing and the Grand Wing. The Terrace Wing Grand Rooms feature light-coloured timber interiors. Amenities for business travellers include a functional extended workstation, high-speed cable and wireless broadband networks and safes designed to accommodate notebook PCs and other business communication devices. Once stored, notebooks can additionally be recharged. Other amenities include a 37-inch LCD television with personal audio/video connection, including a DVD/CD/VCD player, an in room iron/ironing board, a hair dryer, a mini bar and individually adjustable air conditioning. The Grand Wing Grand Deluxe Rooms offer separate bedroom and living areas, with flat screen digital Bang & Olufsen TV and a high-tech workstation with a cordless desk telephone, built-in, high-speed, broadband Internet access as well as 24-hour power outlet provision that will allow the user to store and charge their laptop at the same time. Each room comes with an en suite bathroom, as well as a radio and tea and coffee-making facilities. A king-size bed also comes as standard in all rooms.
The hotel's brand new spa and fitness centre, Damai reopens extends over 7.246,4371 m2. This new lifestyle club offers uplifting spa facilities and an innovative Fitness Centre. The 130,000 m2 spa comes with 11 nature-inspired spa treatment rooms, complete with an outdoor garden and water features, while the fitness centre is equipped with 18 pieces of cardio and conditions equipment as well as two kinesis stations. Other recreation facilities include a swimming pool, tennis and badminton courts, studio and Oasis restaurant.
Dining options include Mezza 9 - which offers guests nine unique dining concepts divided into individual sections, featuring Asian and Western cuisine, Straits Kitchen, a Singapore-inspired restaurant serving up local cuisine in a contemporary market place setting, the hotel poolside restaurant Oasis, featuring a weekly BBQ buffet dinner, and Pete's Place- an authentic Italian restaurant serving homemade pastas and wood-fired oven pizzas.
American Express, Diners Club, JCB, MasterCard and VISA are all accepted as methods of payment.
The hotel reception counter is open 24 hours daily. Photo identification and credit card or cash deposit are required at check-in for incidental charges. Special requests are subject to availability upon check-in and may incur additional charges. Extra-person charges may apply and vary depending on hotel policy. Official check-in time is 14:00 h and check-out is 12:00 h. Pets are not allowed in the hotel.
Room Facilities:
Direct dial telephone
Satellite cable TV
HiFi stereo system
Internet access
Tea Coffee
Iron and board
Double bed
King size bed
Central airconditioned
Wheelchair accessible
Disabled bathroom


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protect the integrity of your idea and your brand.
Product packaging serves as the "face" of your brand. Bold and clever packaging solutions distinguish your brand, entice customers, and build brand equity. When promoting everyday or luxury products, Fortune 500 companies face the same challenge of ensuring brand consistency and quality across products and markets. They often contend with long lead times, high packaging costs, and a lack of innovative approaches.
from everyday to luxury brands, InnerWorkings develops custom packaging strategies that modernize and refine your marketing operations.
To solve these challenges, InnerWorkings introduces transparency, creates new sourcing and design strategies, and removes inefficiencies throughout the traditional marketing supply chain for all types of packaging – from everyday to luxury brands.
For years, we have received numerous honors, including Luxury Packaging Awards in London as well as accolades from our discerning clients. Perhaps the most rewarding is our client retention rate of over 98%, including customers that have been with us since our inception.
recent packaging resources
Steve Schoultz, Vice President of Packaging at InnerWorkings, was recently quoted in "Beverage World" about the latest packaging trends.
in our own words
differentiate your brand through innovation
Let's discuss your needs.


text:
Mario Party 9 Wii: New Mini Games
Mario Party 9 will be released on March 11, 2012.
In this review I'll be showing the Free Play Mini games for Mario Party 9.
I pick Mario for my player and choose Princess,Toad, and Luigi for my competition.
Mario Party 9 First Mini game – Buddy Bounce
"Bounce on the clouds to collect as many ballons as you can. Bouce on the head of another player to reach the higher, more valuable balloons."
Your just trying to collect as many point balloons as possible to get the balloons with more points you need to time your jumps so you bounce off your competitors heads. Your earn 1 Party Point
Mario Party-9 Second Mini game – Logger Heads
Rules: Use your ax to be the first to split the logs placed in front of you. Look out for Bob-ombs that explode if you hit them by mistake!
This game is similar to whack a mole type, you use the Wii mote swinging in a downard motion to be the first one to hit the log of wood, careful not to swing on the bomb or you loose.
Mario Party-9 Third Mini game – Pinball Fall
Rules:Choose your ball, then let luck take over as you roll downhill. Be the first to reach the bottom to win!
This game reminds me of the price is right plinko game, it's basically a game of luck, however the only thing you can actually control in this one is when you get stuck in a hole swing your Wii mote side to side to get out quicker than your opponents.
Mario Party-9 Fourth Mini game – Launch Break
Rules:Use the controls on-screen to build up energy in your three-stage rocket. The player who blasts his or her rocket highest wins!
This is a great mini game, first step is to tap button 1 on your wii mote while holding it sideways then switch to button 2 and at the last stage shake your Wii mote side to side as fast as you can. Afterwards your rocket's blast off in a three stage animation.
Mario Party 9 Fifth Mini game – Goomba Village
Watch the Goombas as they run into the different Toad Houses. Try to be the first player to guess which Toad House holds the most Goombas!
Focus is everything here, pay close attention to where the Goombas walking towards and at the end you need to decide quickly which house is the winner.
Watch In depth game play footage of EVERY SINGLE Mario Party 9 Mini Game in this series.


text:
Mobile App
LKCE Jalandhar
Lyallpur Khalsa College of Engineering
Total Students
Fees Range
How to reach
Lyallpur Khalsa College of Engineering is situated at Cantt. Road, Jalandhar City, Punjab, Pin-144001 in Ludhiana.
Courses Offered
- 4 Years
- Bachelor of Technology
- Engineering
- 4 Years
- Bachelor of Technology
- Banking / Finance / Accounts / Commerce
- 4 Years
- Bachelor of Technology
- Engineering
- 4 Years
- Bachelor of Technology
- Engineering
Lyallpur Khalsa College of Engineering - LKCE Jalandhar - LKCE
The Lyallpur Khalsa College of Engineering got established in 2013 under the aegis of Khalsa College Lyallpur Education Charitable Trust. The Institute is located in the heart of city on a prime piece of land. It was established with an aim to impart engineering education to the students to make them competent, motivated engineers and scientists. The institute as a first step will provide the education in the field of Mechanical Engineering, Civil Engineering, Electronics & Communication Engineering and Computer Science & Engineering disciplines, the most sought after disciplines at present due to the huge potential for the industry absorbing the good students in these fields and very rapid investments taking place in the region. The Institute not only celebrates freedom of thought, cultivates vision and encourages growth, but also inculcates human values and concern for the environment and the society.To contribute to the region & the country at large through innovative excellence in experimental based scientific and technical education with a focus on research so as to be a valuable resource for the corporate world and the humankind and to become a source of pride of all countrymen.To impart quality education and training and to carry out research in consonance with the vision of the Institute, by providing state-of-the-art infrastructure and a learner friendly atmosphere in synergy with innovative pedagogy and global networking; to produce world class and socially responsive academicians, leaders and practitioners in various disciplines.
Why join Lyallpur Khalsa College of Engineering
The institute has modern infrastructure with all latest equipments.
4 Type Courses Available
- 4 UG Courses
Lyallpur Khalsa College of Engineering Placements
The Training & Placement Cell firmly believes in 'Industry-Institute Interaction'. In order to accomplish 'Industry-Institute Interaction', it organizes technical talks and national seminars to provide a platform for the budding engineers to interact with professionals from various industries. It encourages visits to the industries by the college students. It arranges for industrial problems to be worked on by students as part of their projects. It also takes suggestions from members of industry regarding designing/changing of curriculum. The college has signed MOUs with many industrial organizations and industrial chambers. Technical paper presentation contests are held in collaboration with industries to discuss new developments and trends.
Visiting Companies
Student Placement
At Lyallpur Khalsa College of Engineering the students are endowed with modern infra and convenient amenities that make their life 'go easy' in college hours, alongside make learning more interesting. Some of the facilities include well ventilated classrooms, recreational areas, hostel facility for boys and girls, indoor and outdoor games, stocked library, seamless internet connectivity, medical facilities etc.
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text:
New Delhi, Nov 15: Minister RS Katheria is in double trouble. While the rumors of forged marksheets is yet to die down, he has gotten himself in murkier grounds with another case of forgery in work experience too.
[See also: Cabinet reshuffle: A junior minister of Education blamed of forging his marksheet]
A chargesheet against the 20-day old junior minister of state, education, stated that he has not only forged two university marksheets to land a teaching job at Agra University, but also a work experience document.
The minister, however, said,"If the charges are proven, I will not just resign as minister but also as a parliamentarian."
The second charge on him states that he has falsely claimed to have worked in an Etawah college as a Reader for 3 years since 1999. The state police alleges that investigations show that he never worked at the Etawah College.
Naming it a political vendetta by the rival Bahujan Samaj Party candidate who lost narrowly to him in the 2009 national elections. In fact, in 2012, the Allahabad High Court dismissed Mr Katheria's attempt at getting the case quashed and directed the Agra sessions court to resolve it as soon as possible.
Katheria has mentioned just one case out of 21 cases in his election affidavit. If the charges are proved against him, he may have to serve a jail term of 7 years.
OneIndia News


text:
Quick Look
Property brokered by
Get more listing details on brokerage site
Property DetailsLocated at the end of one of darien'S most coveted cul-De-Sacs, this spacious five bedroom home has what every buyer is looking for. With hardwood floors throughout, the living room has a fireplace and the den has built-Ins and french doors leading to a gracious patio with stone sitting walls. A large dining room and a separate breakfast room provide both formal and informal dining spaces. The updated kitchen overlooks a large family room complete with a fireplace, wet bar and built-Ins. for your au pair or guests there is a separate suite and playroom over the garage. a large flat yard and distant views of the golf course make this the perfect home for you.
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Additional Property InformationThis listing located at 16 Stonewall Lane, Darien, CT offers five bedrooms, four bathrooms and two partial bathrooms. The property is currently priced at $1,999,999.
This property is presented by Houlihan Lawrence Real Estate based in New York.


text:
I'm Sorry
Yes, The consequences are known,
Alas, I cannot help the way I feel,
The blood I bleed, Air I breathe,
All I do, Just for you,
The world knows, Who we are,
Incinerated Lovers.
Unable to carry on this way,
To live without you, Impossible,
Spikes, Chains, Even the pain,
All of this, I miss, And more,
It cannot be helped, Still in love with you, I find myself,
You are my rising, Whilst I am falling,
You know my situation, Complications,
Another would be inadequate, Inappropriate,
Only you,
Thorns from the rose, Lace my heart with fear,
Cure me, I beg you.
Black roses, My passion, Red, My fire,
Together with you, creating a love even death is unable to cease,
I cry for you, Reach for the blade,
A calming liberty, I bleed,
Listen, I slit,
Listen, For the blood,
These feelings, Unavoidable, I cannot ignore,
Staining my soul, My love for you burns inside,
Give me reasons.
I think of you, Always,
Bleeding inside, I bleed through, Bleeding my love to you,
I need you, No matter what may be said,
My friends, Family, I betray,
You've become my sanctuary, Unsafe unless I'm with you,
Venomous feelings I ignore, Heart-bourne ones I pledge to you.


text:
Crows Nest Bed & Breakfast
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
- Restaurant
- Laundry facilities (self serve)
- Bar/lounge
- Free parking
TripAdvisor Reviews Kangetsuen Otofuke-cho
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Transported to Van Diemens Land
... home for the next four nights. This meant two flights, a four-hour wait at Melbourne airport and a 90-minute time jump. And did we mention the contrast in weather? It feels like we're back home. Although the view from the window looks more like Scotland than the Chilterns. Could just be the lowering cloud….
After the first 20 minutes of bush from above you turn to your Kindle – M's reading list has included the autobiographies of Tom Jones (entertaining ...
... one of the guides about the installation featuring a China bowl with knife and two goldfish placed on a chair. OK, so is this art if anyone could do it? Are the goldfish OK? Is this a metaphor for the fact that life is like living on a knife's edge every day? Is there any skill or talent involved in this at all? She reckons she 'hated' everything at MONA but I have set both children the task of writing a persuasive text arguing the point about the validity of normal objects ...
Tasmania - The Jimi Hendrix Experience
... once again cooked by our lovely female companions, and just sat around the fire having a drink. However conversation stopped as soon as it got really dark, the sky erupted with light as what seemed like every star in the solar system came out to shine for us. Shooting stars shot across the blackness and as we sat there, I was so glad I came on this trip. I love nature, camping, and generally being out doors. So to be back out in the nature scene for a week was awesome for me. However ...
... adjoining the graveyard of a church where paupers and convicts were buried in unmarked graves...these convict had toiled in atrocious conditions to build the church....Somehow, I had a slight suspicion that Rosie was also the one responsible for nicking a few plastic flowers from other graves and planting them under her humble wooden convicts cross. Finally reaching Hobart it seemed little had changed since I was last there fifty years ago. It took me no time to navigate my way ...
The Land Under the Land Down Under... Tasmania!
... the car, what had started as a 30 minute walk to the lookout, turned into an 11km trek. That night the rain storm hit, as promised, and we woke up to soaking rain with most of the island socked in. We had planned on continuing up the east coast but decided there wasn't much point since we couldn't see more than a couple hundred feet through the rain. The forecast for the west side of the island looked more promising so we started the 6.5 hour drive to one ...
Other places to stay in Hobart
Gattonside Heritage Accommodation51 - 53 Sandy Bay Rd, Hobart | B&Bfrom $156
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- Lovely little guesthouse
Signalman's Cottage B & B685 Nelson Rd, Hobart | B&Bfrom $32
The Elms of Hobart452 Elizabeth St, Hobart | B&Bfrom $132
Bendalls Bed & Breakfast80 New Town Rd, Hobart | B&Bfrom $109


text:
Link Building Tips
Consider the good that you can create with your site, if you try to build links. Technology link building is a great way to make sure that your website is getting more attention from people who are searching for specific terms. The more links you get, the more relevance that your site can get from those who actively search for web sites and other sources of information hierarchies. You could create a...
Make Sure Social Media is the Central Source of Your Weblink Building
Since Search engines presented the Panda and Penguin many individuals believe that backlink developing, and SEO for that issue, are deceased. Are they? No, this is certainly not the situation. Here is why sites that are currently rated on the first web page of Search engines are there: they have powerful energy hyperlinks. They would not be there if they had poor, low value hyperlinks. However with the...
Tips to Improve Local SEO
Local SEO—or LSEO as I like to call it—is changing easily. For example, Look for engines recently began the procedure of developing its old local listing product Look for engines Places into Google+ Regional. Also, just within the last two weeks or so Look for engines declared the launch of a Google+ Regional Dash panel for managing company webpages, which provides ideas, top search search phrases, and...
SEO Major Indicators: The Best Way to Improve Your Per month Customer Reports
Economists regularly use leading signs — the considerable economic aspects that modify before the economic system as a whole changes — in order to evaluate and estimate future performance. Lagging signs, on the other hand, are aspects that modify after the economic system as a whole does. Since Local SEO is generally a long-term strategy, customers tend to evaluate achievements depending on what would be...
Social Media & Get Started with Social Networks
An online social network is much like your actual online community. It is your buddies and you members , work colleagues and acquaintances. A social network is a way of staying in touch and making new relationships. In actual life you can't see the relationships between individuals. In the internet you can immediately see who knows who, and who your buddies are buddies with. sharing images with your...
Social Media Marketing Art
Enticing traffic to your website through the use of entertaining social systems like Facebook® and Twitter® is called social media. You can use social media systems (Facebook, Tweets, Google, LinkedIn®), social media discussing (Pinterest®, YouTube®, Flickr®), and social bookmark management (Digg™, StumbleUpon®) to declare news about your company, and produce enjoyment about your web page. Social social...
Improve Your Website Traffic With Social Media
I have distributed Few Public Press Discussing Gadget and a technique about Automatic Publish Discussing to several Public Press Web page, because sharing our post to Public Press is produce guests for us from our group. However lovers buddies or fan we have, our post will instantly showed up on their surfaces. As we have already described about it and its also noticed the most variety of individuals...
SEO in 2013 – What Knowledge Professionals
SEO strategies are evolving all the time. The days are gone forever Public data on your keywords and link building mass. Despite the many difficulties encountered along the way, but we were able to gain a few tricks up his sleeve. One of them is the justification for the existence of the person responsible for positioning. Advertising expenditure on SEO campaigns are growing and the largest share of all...
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My Content
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Track listing: Boplicity; Hidden Agenda; Bas Relief; Israel; Mode to John; Just Blues; Moondreams; Mystic Valley (53:21).
Greg Hopkins, conductor, trumpet; Gary Carney, Russell Scarbrough, trombone; Sam Pilafian, tuba; Scott Zimmer, alto sax; Bryon Ruth, alto, tenor sax; Steve Von Wald, baritone sax; Chuck Marohnic, piano; Ed Friedland, bass; Dom Moio, drums.
Contact: Summit Records, Box 26850, Tempe, AZ 85285
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