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It only takes a minute to get started. Fill out our simple sign up form and login to the site.
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A Premier's State
€ 26,99
epub eBook
Sofort lieferbar (Download)
August 2012
Beschreibung'In May 1994, while I was going through pre-selection for the seat of Williamstown, I sat down at my desk at home and I wrote a note. I was thirty-nine years old and in that note I mapped out what I hoped would happen in my life.'By the time he was forty-eight, Steve Bracks had achieved the goal he'd set himself nine years earlier. He was premier of Victoria. In A Premier¿s State he reflects on his ambition to make a difference, and how he reached his goal. He talks about his early childhood growing up in a conservative but impassioned family that supported the Democratic Labor Party, and about his gradual evolution from left-wing university radical to pragmatic centre-left premier. He reveals for the first time the background to his decision to take the party's leadership from his friend John Brumby in 1999-then to hand it back to John in 2007 when he sensationally resigned from office. He gives insights into how to run a successful government and how to manage the factions, and talks about everything from the impact of public life on his family, to forming minority government with independents.
Untertitel: Sprache: Englisch.
Verlag: Melbourne University Press
Erscheinungsdatum: August 2012
Format: epub eBook
Kopierschutz: Adobe DRM

subset: commoncrawl


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My week has been one of experiencing the meaning of the word 'between' (Not to be confused with the town of Between, GA which lies exactly between my parent's house in Atlanta and my most recent house in Athens). I have experienced and relished the state of being between, which I have conveniently organized into paragraph form for you. Yes, you.
The first state of between was geographical: between Kiva training in San Francisco and the start of my Kiva Fellowship in Peru and Bolivia. I am one of the first in the 6thClass of Kiva Fellows to go...Continue Reading >>


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Chalet in La Chapelle d'Abondance, Haute-Savoie, Rhone-Alpes, France - High Quality Accommodation To Provide A Base For Winter Or Summer Activities.
Chalet Max is a typical alpine style building on the outside. The interior has been fitted out and furnished to give a contemporary feel and provide all comforts of a modern home. The Chalet will provide its guests with a calm and peaceful holiday and is a great base to experience the joys of outdoor life in the Alps. The location is ideal for access to the 'Portes du Soleil' skiing area, one of the largest in Europe, straddling the border between France and Switzerland and offering a wide variety of skiing opportunities, from absolute beginners to adrenaline seekers and off-pisters. In addition there are numerous snowshoe and cross country ski trails which pass the back garden. Summer activities are amazing too: mountain biking, road cycling, walking, white water rafting to name but a few. Alpine food is wonderful and being situated in the Abondance valley, a traditional farming area, copious amounts of delicious local cheeses are readily available to try and to buy.
Top Traveller Review
Highly recommended by all our party
The first thing that struck us about Chalet Max is how well-appointed, spacious and well-equipped it is; clean, stylish and homely. 10 people were accommodated comfortably with a good ratio of beds to bathrooms, and the garage was ideal for equipment storage. The owners have excellent local knowledge and provide useful recommendations.
Chatel is a fantastic place to ski; plenty of skiing for all levels, and great touring. Several excellent nearby restaurants frequented.
About the Owner
About Mark Perriton
Mark and Lottie built Chalet Max in 2006 and we have been using it for our own holidays, coming at least 6 times a year, in all the school holidays. We love the area and are still discovering the fantastic diverse range of activities at different times of the year.
Mark Perriton purchased this chalet in 2006
Why Mark Perriton chose Portes du Soleil area
The chalet is situated in the commune of La Pesse, between the small towns of La Chapelle D'Abondance and Chatel both offering ski lifts with access to the whole Portes du Soleil ski area. It is in a quiet spot with a river at the end of the garden, spectacular mountain walks starting from the chalet, tennis courts almost across the road and a good supermarket 5 minutes away!
5 Reviews
4.8/5 -
(5 traveller reviews)
Highly recommended by all our party
(2 winter visits in Feb and April 2015)
The first thing that struck us about Chalet Max is how well-appointed, spacious and well-equipped it is; clean, stylish and homely. 10 people were accommodated comfortably with a good ratio of beds to bathrooms, and the garage was ideal for equipment storage. The owners have excellent local knowledge and provide useful recommendations.
Chatel is a fantastic place to ski; plenty of skiing for all levels, and great touring. Several excellent nearby restaurants frequented.
Beautiful chalet in great location
We've stayed at Chalet Max twice now - once during October when we enjoyed some spectacular hiking, and once in February when we skiied at nearby Chatel and Pré La Joux. The chalet is very comfortable and clean, extremely well equipped, and fabulously located next to a river and just a very short drive from La Chapelle, Chatel, and great ski fields and hikes. The owners are really friendly, generous and helpful. An ideal summer retreat if you like hiking, mountain biking or climbing, and an awesome winter location for snow sports.
Spacious chalet
Chalet Max has plenty of space and is very comfortable. An excellent location - not just for skiing but also for walking and cycling in the mountains. (It's worth cycling up to Col de Bassachoux). Tennis courts are also nearby. So endless options! Thoroughly recommended.
Great accommodation
Lovely, well appointed chalet close to ski lifts and restaurants. Chalet is very spacious and well equipped. Owners extremely helpful and friendly. We loved the location, we used our hire car to get to ski lifts, but bus stop right outside chalet.
High quality accommodation
We've enjoyed two great skiing holidays staying at Chalet Max. Unusually for alpine resort chalets it is extremely well appointed and very comfortable, clean and well maintained - important to our party which includes non-skiers. Two good restaurants are within a 20 minute walk so no one has to forego a glass of wine with dinner. We like the quiet location and the river at the end of the garden. Its also useful that the drive is level so not a danger to grandparents when it snows.
From the Owner
"The chalet is situated in the commune of La Pesse, between the small towns of La Chapelle D'Abondance and Chatel both offering ski lifts with access to the whole Portes du Soleil ski area. It is in a quiet spot with a river at the end of the garden, spectacular mountain walks starting from the chalet, tennis courts almost across the road and a good supermarket 5 minutes away!"
Activity and Interests
Special interest holidays:
The chalet is also a great base for summer breaks. Numerous well marked mountain paths start at or near the chalet, making it a haven for walkers; mountain biking is very popular here in one of Europe's best mountain bike areas served by the winter ski lifts; La Chapelle d'Abondance has its own Via Ferrata rock climb or you could try Chatel's infamous 'Fantasticable', sliding 240 metres above the valley along 2.5 Kilometres of wire. White-water rafting, canyoning, hydrospeeding, kayaking etc are all available from May to September.
Other activities include: tennis courts a short walk away, horse riding, swimming, fishing.
At Chalet Max we can offer a range of activities and courses for groups of people who want to have a good time but also learn some new skills. We can arrange for a dedicated teacher to look after the needs of a group who want to learn to cook, improve their language skills, paint and draw, or learn more about the Alpine flora and fauna.
From 40 minutes drive from Chalet Max are the beaches of Lac Leman - St Gingolph, Evian, Thonon and at Geneva itself.
Nearest swimming pools: for summer there are outdoor pools in Chatel, Morgins (10 mins drive), Evian (40 mins drive), St Gingolph (40mins) and some lovely spa pools in Switzerland (Val d'Illiez or Lavee les Bains - 30/45 mins away by car) for all year round. A lovely lake with zip wire is located at Bernex (30mins drive)
The chalet is one and a half hours from Geneva airport by car.
Access to the Chalet from Calais by car is 10 hours drive.
There is a choice of trains and local buses to access the chalet if public transport is preferred.
Once in the Chalet then a car is ideal. There are ski bus stops outside the Chalet and local buses to explore the valley.
Chalet Max is 3 km from Chatel and Chapelle d`Abondance. To explore Switzerland the border is 5 Km from the Chalet.
Chalet Max is one and a half hours from Geneva.
Further Details:
Numerous restaurants are accessible within a short drive - some details of our favourites are in the chalet.
If you like out door life then Chalet Max is the perfect base all year round. In summer the location is great for walkers, bird watchers and nature lovers. Beautiful landscape is on your door step ready to explore.
The village of Chatel offers a wide range of shops and activities to suit all needs.
There is the sophistication of Geneva and Lac Leman for culture and sailing an hour down the road.
Prices and Availability
Rental rates
|Rental Period
|Minimum Price
|Additional Nights
|Weekend Night
|Event Price
16 Apr 2017 - 1 Jul 2017
6 night minimum stay
for 6 nights
2 Jul 2017 - 26 Aug 2017
6 night minimum stay
for 6 nights
27 Aug 2017 - 28 Oct 2017
6 night minimum stay
for 6 nights
out of season
29 Oct 2017 - 15 Dec 2017
5 night minimum stay
for 5 nights
16 Dec 2017 - 6 Jan 2018
6 night minimum stay
for 6 nights
7 Jan 2018 - 10 Feb 2018
6 night minimum stay
for 6 nights
Additional pricing information
|No additional mandatory fees
|Refundable Damage Deposit
Booking Notes:
A non - refundable deposit of 50% is required to secure the booking. The balance is payable 4 weeks before arrival. A security deposit of 500 Euro (£450) is required (payable with the balance payment) and will be returned within 14 days of departure providing there has been no damage to the chalet.
A deposit is required for use of the bikes.
Changeover Day:
Saturday - arrival from 4pm; departure by 10am.
Other days possible - contact us to discuss.
Notes on prices:
Other rental periods available - please contact Mark or Lottie.
Owner's Cancellation Policy
- 50% refund for cancellations more than 30 days before check-in date.
- 100% refund for cancellations more than 60 days before check-in date.
- 12
- Available
- 12
- Unavailable
Property Type
- chalet
- Heating
- Internet
- Iron & Board
- Living Room ...
- Parking
- Tumble Dryer
- Washing Machine
- Dishwasher
- Freezer
- Kitchen ...
- Microwave
- Oven
- Refrigerator
- Hob
- Dining Area
- suitable for elderly or infirm
Location Type
- mountain
- river
- Master bedroom with en-suite shower and WC, shower room with hand basin, Bathroom with separate shower and hand basin. Separate WC on the ground and first floor.
- 5 Bedrooms, Sleeps 10
- Description of sleeping capacity: Sleeps 10 (5 Bedrooms) Notes on bedrooms: Master bedroom - King size bed, bedrooms 2, 3 and 4 with twin beds and bedroom 5 with adult sized bunk beds.
- Garden
- Barbecue
- Ski & Snowboard ...
- Self-catering
House Rules
- Max. occupancy: 10
- children welcome
- Notes on accommodation: Chalet Max is a very comfortable place to stay with loads of natural daylight and space to switch off from life at home. The location of the Chalet allows access to the supermarket within 2 minutes in the car; there are public tennis courts within a couple of minutes walk from the Chalet. Chatel and Chapelle d'Abondance are 5 minutes drive. Outside: The Chalet has its own large grassed garden with a stream running through the middle and the river Dranse running along the end boundary.There is a patio area with garden furniture ideal for a barbecue and outdoor dining and sunbathing. Utility Room: Washing Machine and Dryer. Cleaning / Towels / Linen / Maid service: Towels and Linen are hired per person at the time of booking. Please advise how many double beds and single beds are required. Cleaning at the end of the rental period is included within the rental cost. Other: Large garage with car port. Parking for 2 more cars at front. 3 comfortable "hybrid bikes" are available on request and an electric assist bicycle, ideal for cycling on hilly roads providing exercise with assistance!


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VR Software
Virtual reality is immersive, engaging, and fascinating. The ability to stroll the green at Vanderbilt University or get a mountaintop view of Rio de Janeiro, all without leaving your home, is a powerful tool for creating, entertaining, and learning.
And as you can imagine, it can be incredibly complicated to create virtual reality content. However, new platforms such as YouVisit's Experience Builder have been developed to make the process faster, easier, and accomplishable by general users.
While Experience Builder lets you jump right into creating VR content, it is helpful to understand the traditional steps to the process, especially VR software.
Capturing the Images
Virtual tours begin with photography, with the goal being to capture a 360-degree perspective of a given place. Fish-eye lenses work well for this task, as they can capture the full perspective an experience in as little as two or three photographs.
For the high-resolution images needed for the most immersive experience possible, however, the capture process is more complicated. The camera should be at least 16 megapixels and stabilized with a tripod or other mount. To get the best looking tour possible, thousands of images may might need be captured, depending on the camera's focal length.
Stitching Images
Those individual images are then connected together, or "stitched," creating a three-dimensional perspective–like the inside of a tennis ball. Editing is usually required before this process, to white balance the images, remove optical distortions, balance colors, and to remove the tripod. Once the images are made uniform, a designer uses programs like Photoshop's Photomerge, PTGui 10, Panoweaver, or others to stitch the individual images into one seamless, panoramic display.
Final Touchups
Even after stitching, the panoramic image may need extensive edits to remove imperfections and distortions. Stitching marks must be removed, color conversion is performed, and the final version is compiled to make it compatible with multiple browsers.
Virtual tours are generally viewable via Flash, Javascript (compatible with smartphones and tablets), HTML 5 (universally compatible), or Quicktime VR. This conversion is done with software such as Panotour, Tourweaver, Pano2VR, or Cubic Converter.
Experience Builder
While camera gear, editing software, and proper training cost easily thousands of dollars, Experience Builder provides a platform to streamline and enhance the creation tour tours and virtual reality content.
After creating a free profile, a user uploads his or her own files, which can include images, videos, or panorama files. Users also have the options of using a Richo Theta to capture 360-degree panoramas, which is compatible with Experience Builder's smartphone app.
Experience Builder allows users to arrange various locations in different orders to create "stops" along their virtual tour, and designate "hotspots" within the image—points of interest that can be highlighted with other media, including text and two-dimensional photos that can be linked to online content. These stops can also be linked and tracked through Google Maps.
More importantly, Experience Builder allows users to create content for all leading virtual reality headsets—meaning they do not need to know each devices particular VR software.
Completing a virtual tour from start to finish can be a lengthy, complicated process. But tools like Experience Builder can do much of the technical legwork for you, supporting the quick and easy creation of a beautiful, immersive three-dimensional experience.


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JM Studios Photographer
Miss Jolene RoseModel Female Lawrence, Kansas, US
My MM URL: http://www.modelmayhem.com/missjolenerose
Mayhem # 2409698
About MeI am a vibrant young woman who is looking for more opportunities to do what I love to do. I eat healthy and am proud to say that I am in pretty good shape. I am not very tall, and I am not pin thin, but I love my body. I embrace my curves and I figure I might as well flaunt them while I'm young. I am a passionate person, and I want my modeling to show that. When I am modeling, I give it every thing I've got. I want my pictures to reach out and grab people. I am willing to try pretty much any type of modeling.
*Please no drive-by friend requests. Leave a tag, comment on a picture, or send me a message. Please and Thank you. =D
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Popular property highlights
Located in Hoan Kiem, Hanoi La Siesta Diamond Hotel is adjacent to Thang Long Water Puppet Theatre and within a 10-minute walk of other popular attractions like Hoan Kiem Lake. This 34-room, 3.5-star hotel has free in-room WiFi along with a rooftop terrace and a 24-hour business center. Located in central Hanoi, the hotel is also near Temple of Literature and Ho Chi Minh Mausoleum.
Free breakfast is included with your stay. Enjoy a bite to eat at Gourmet Corner, the hotel's onsite restaurant. Order from room service to satisfy your hunger without leaving your room, get a quick pick-me-up at the coffee shop/café, and mingle with other guests at the free manager's reception.
The 34 rooms are soundproofed and have an array of amenities, including minibars, coffee makers, and computers. 32-inch LCD TVs come with cable channels and DVD players, and guests can stay connected with free WiFi and wired Internet. Pillowtop beds sport premium bedding and bathrooms offer hair dryers and free toiletries. Free newspapers, free bottled water, and safes are also standard.
Guests of Hanoi La Siesta Diamond Hotel enjoy a rooftop terrace, free WiFi in public areas, and a meeting room. For transportation, you can make use of an airport shuttle (available 24 hours) for USD 23 per vehicle one way, as well as a train station shuttle. The front desk has multilingual staff on hand 24/7 to help with dry cleaning/laundry, tours or tickets, and luggage storage. Other amenities at this luxury hotel include a 24-hour business center, coffee/tea in a common area, and express check-in.
Property features
- Walking distance to Thang Long Water Puppet Theatre and Hoan Kiem Lake
- Free breakfast
- Free WiFi and wired Internet in rooms and public areas
- Onsite dining
HighlightsInformation missing or incorrect? Tell us!Opens in a new window
Hanoi La Siesta Diamond Hotel
Hotel Amenities
Hotel Amenities
Dining options at the hotel include a restaurant and a coffee shop/café. A complimentary breakfast and manager's reception are offered (may not be available daily). Public areas are equipped with complimentary wired and wireless Internet access. Business-related amenities at this 3.5-star property consist of a 24-hour business center and a meeting room. For a surcharge, the property provides a roundtrip airport shuttle (available 24 hours) and a train station pick-up service. This Art Deco hotel also offers a rooftop terrace, spa services, and tour/ticket assistance.
Hanoi La Siesta Diamond Hotel has designated areas for smoking.
- 24-hour front desk
- Babysitting or childcare (surcharge)
- Airport transportation (surcharge)
- Total number of rooms - 34
- Number of floors - 12
- ATM/banking
- Coffee/tea in common areas
- Coffee shop or café
- Free newspapers in lobby
- Free breakfast
- Dry cleaning/laundry service
- Elevator/lift
- Express check-in
- Express check-out
- Gift shops or newsstand
- Tours/ticket assistance
- Free WiFi
- Free wired high-speed Internet
- Designated smoking areas
- Luggage storage
- Spa treatment room(s)
- One meeting room
- Multilingual staff
- Rooftop terrace
- Porter/bellhop
- Restaurant
- Safe-deposit box at front desk
- Train station pickup (surcharge)
- Free reception
- Year Built 2010
- 24-hour business center
- Shopping on site
- Spa services on site
- Number of buildings/towers - 1
Family Friendly Amenities
- Family Hotel
- Free Wi-Fi
- Soundproofed rooms
- Babysitting or childcare (surcharge)
- Cribs/infant beds (complimentary)
- DVD player
Available in all rooms: Free WiFi , Free wired high-speed Internet
Available in some public areas: Free WiFi , Free wired high-speed Internet
Room Amenities
Hanoi La Siesta Diamond Hotel offers 34 air-conditioned accommodations with computers and DVD players. Pillowtop beds feature premium bedding. 32-inch LCD televisions come with premium cable channels. Guests can make use of the in-room coffee/tea makers and minibars. Bathrooms include showers, slippers, complimentary toiletries, and hair dryers.
Guests can surf the web using the complimentary wired and wireless Internet access. Business-friendly amenities include desks, complimentary newspapers, and safes. Additionally, rooms include complimentary bottled water and irons/ironing boards. A nightly turndown service is provided and housekeeping is offered daily.
- Premium TV channels
- DVD player
- Premium bedding
- Air conditioning
- Free newspaper
- Minibar
- Coffee/tea maker
- Free bottled water
- Daily housekeeping
- Turndown service
- Private bathroom
- Free toiletries
- Hair dryer
- Slippers
- Shower only
- Desk
- Iron/ironing board
- Blackout drapes/curtains
- In-room computer
- Cable TV service
- Free WiFi
- Free wired high-speed Internet
- Soundproofed rooms
- In-room safe
- Room service (limited hours)
- Pillowtop mattress
- Free cribs/infant beds
- Rollaway/extra beds (surcharge)
Where to Eat
Room service (during limited hours) is available.
A complimentary breakfast is offered. The hotel offers a complimentary manager's reception on select days.
Gourmet Corner - This restaurant specializes in Vietnamese cuisine and serves breakfast, brunch, lunch, and dinner. Guests can enjoy drinks at the bar. Reservations are required.
Nearby Things to Do
The recreational activities listed below are available either on site or nearby; fees may apply.
- Ecotours nearby
Spa Services
- Hot stone massage
- Swedish massage
- Reflexology
- Body wraps
- Body scrubs
- Massages
- Body treatments
- Facial treatments
Hotel Policies
Check-in time ends at midnight
Check-in time starts at 2 PM
Minimum check-in age is 18
Special check-in instructions:
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Kids stay free! One child 4 years old or younger stays free when using existing bedding.
- Rollaway/extra beds are available for USD 15 per night.
- Free cribs (infant beds)!
- No pets or service animals allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No pets or service animals allowed
- Resort fee
Optional extras
- Airport shuttle fee: USD 23 per vehicle (one way)
- Rollaway bed fee: USD 15 per night
Hotel Name
- Diamond Elegance Hanoi
- Diamond Hanoi
- Elegance Diamond
- Elegance Diamond Hanoi
- Elegance Diamond Hotel
- Elegance Hanoi Diamond
- Hanoi Diamond
- Hanoi Diamond Elegance
- Hanoi Elegance Diamond Hotel
We should mention
This property offers transfers from the train station (surcharges may apply). Guests must contact the property with arrival details 24 hours prior to arrival, using the contact information on the booking confirmation. A resort fee is included in the total price displayed. One child 4 years old or younger stays free when occupying the parent or guardian's room, using existing bedding. Only registered guests are allowed in the guestrooms. No pets and no service animals are allowed at this property.
Awards & Affiliations
- The property is a member of the Small Luxury Hotels of the World.


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Big Bead Set of Four Spreaders
We're sorry, but this particular item has sold out online, so you will not be able to add it to your shopping bag. Please try a different color or size, if applicable.
Display and generously glob on butters, jams, cheeses, and more with this exquisite set of four spreaders! Perfect for casual use or for more formal gatherings, this collection is made for everyone to sample their favorite sauces.
- Set of 4
- 5.25-in. L
- Alternative Metal
- Hand Wash
- Belk Exclusive
- Imported
Shipping & Returns
Shipping your purchase is easy on belk.com. Depending on the destination of your package, you can expect it to arrive within 4-7 business days. Shipping Information
Returns are easy on belk.com. Easy returns


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Follow Harriet on Twitter
About Harriet
- The Poetry Foundation's blog for poetry and related news.
- More about Harriet
- Contributors
- Archive
Posts Tagged 'Álvaro Mutis Jaramillo'
Rest in Peace, Álvaro Mutis Jaramillo September 24, 2013: The poet and storyteller passed away in Mexico City on Sunday: he was 90 years old. From the Guardian: Prolific Colombian writer and poet Álvaro Mutis Jaramillo has died in Mexico City. He was 90 [...]


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Call 0333 200 2024 lines open Mon to Fri - 9am to 6pm
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Be the first to review this product
Availability: In stock
The Verona Design Antique Pine & Red 3ft High Sleeper Bed by Verona Design is a distinctive piece from the range that would provide an on-trend style, creating a stylish atmosphere. This high sleeper is distinguishable by its solid pine frame with MDF head and foot boards and an antique pine and red finish. This bed comes with a ladder that can be fitted on the left or right hand side, a tall boy wardrobe, six drawer chest of drawers and a pull out desk. The Verona Design Antique Pine & Red 3ft High Sleeper Bed would have no trouble enhancing a contemporary environment.
Please Note: Mattress Sold Separately.
Check items to add to cart or select all
Verona Design Antique Pine & Graphite 3ft Long High Sleeper Bed
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Verona Design Antique Pine & Fuschia 3ft High Sleeper Bed
View the whole Verona Design range.
For more information please call us on 0333 200 2024. Lines are open from Monday to Friday - 9am to 6pm. Alternatively please email us at firstname.lastname@example.com.


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Product: 30 Day Change
Website: www.30daychange.co/
Owner: Simon Crain
Price: Free – (with a big catch)
Rank: Scam..
30 Day Change Review: Scam Or Legit?
Just like today's majority of binary trading system softwares, 30 Day Change got the same 0% rank!
And with all the "Real-Time Profits" scrolling news up top, you're probably thinking the same thing right now, unless of course, you're brand to the world of binary trading system. In that case, no need to point fingers!
This review will thus, be covering for you everything about 30 Day Change:
– The Price
– The System
– The Software
– "Signals" to Why 30 Day Change is a Binary Trading Scam!
Why 30 Day Change is Offered As a Free Binary Software?
As of today, I've written anywhere from 10 to 20 reviews of some of the latest most awful binary trading scams. 30 Day Change, is indeed, no exception to the rule. In-fact, if you're currently reading this review, I believe you're going to be taking away some valuable knowledge for the rest of your life. Knowledge that will help keep hundreds, even thousands of dollars in your pocket.
Nonetheless, while registering for 30 Day Change is absolutely free, in order to get started you're required to pay a $250 minimum deposit. This is used to begin helping you gamble money away with your own assigned binary broker into various binary trading options.
Therefore, because of your $250 deposit, 30 Day Change now being your affiliate in the Binary Trade Options Market is eligible to profiting a commission of your deposit.
You should also know, the reason why this company is completely pointless, is because the fact they are simply taking advantage of consumers by offering no value in return..
30 Day Change System?
Complete B.S.!
30 Day Change Software?
More B.S!
30 Day Change Testimonials?
All Fake!
Do You Keep 100% of The Profits You Make?
Sure you do.. if you win!
Hence, since you're just as likely to throw away $250 down the drain, are just as likely to lose.
30 Day Change Scam – The Red Flags?
This product has some of the most red flags more than any other online product in the binary trading options I have yet to see. Almost as though 30 Day Change found the courage to scoup up all the most infamous scam tactics together and combined them together, to create one awesome (at first-sight only) product.
30DayChange.co Red Flags:
#1 – The first and biggest one that popped out at me, was the top scrolling bar, showing the most recent binary trading winners. You are likely to see amounts in pounds, dollars, euro's, etc.. In addition you're likely to see winnings of anywhere from $100 to $750.
What You Won't See?
You won't see any losses.. Losses which are inevitable in the scheme of things, given the fact we are working within binary trading options!
Thus, it seems as though 30 Day Change has taken the effort to make a product that is very easy to be spotted as a scam from any advance online marketing individual, and a lot harder, for those who barely know what the binary trading options market is.
#2 – "How 167 Average People Went From A Monthly Salary of $3,200 To $46,572.79 In Less Than A Month?"
Now, despite the over-exaggerations made involved with this claim comes something even bigger.. That is, 30 Day Change's "tag line" here, along with the rest of the page layout, looks like its hiding something.. Doesn't it?
For example, where on the page are you able to come to the precise assumption this is a Binary Trading System Software…
Well that's just dandy! In addition, "YOU WILL MAKE HUGE PROFITS AT NO RISK!"
Unfortunately, this was one of the easiest scam products I've had the privlidge of reviewing online. If only they could have made it a tad bit more difficult!
With that being said, let's move on to my conclusion, and look for any better online earning alternatives in store.
30 Day Change Overview
The facts shown within this product proves 30 Day Change to be false, fake, and possibly even illegal for consumer fraud!
Binary Trading Option Systems & Softwares are possibly some of the worst online scams in the internet marketing world, and are what simply give, the reputation a bad name!
Verdict: Scam!
Rank: 0/100
Nevertheless, I have no problem watching these guys go down for this stunt their pulling off and only hope they come to their senses soon enough. This in my opinion, is beyond worse than any possible MLM scheme, or pyramid marketing scam. It's simply over the top and any affiliate who advocates this kind of product won't gain much in the long-term of things. Thanks for reading and happy this helped. Below is an alternative if you'd like to check out; an excellent online opportunity to help you going about building your own legitimate business – can't recommend it enough, newbie or expert! :)


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When to Sell
In the mid-'90s, Vistage speaker Peter Collins surveyed more than 400 business owners (all Vistage members) about their exit plan. Some of the more interesting findings included:
- Three-fourths of the owners had more than 50 percent of their net worth tied up in the business.
- A majority did not know the value of their business.
- Eighty-five percent had no exit plan whatsoever. The 15 percent who did were all over age 65.
- A large majority anticipated selling their business at a "round" age like 50, 55 or 60.
- Eighty-five percent said they would sell now if they were "sick, losing money, the competition was killing them, or they got a great offer."
The really interesting part? These "strategies" almost certainly guarantee a less-than-successful sale of the business.
"Selling your company represents the most important financial decision of your life," insists Collins. "Trust me, this is not the way to do it. The time to sell is when you are on top, not when you aren't enjoying the business or it has hit an economic downturn."
Phillip Currie , a Vistage speaker who runs a San Diego-based merger and acquisition firm, agrees.
"The time to sell is when the future of your company looks brightest," he explains, "not when you've reached a peak or have entered a down cycle. In general, buyers put a lot more value on future potential than on past performance. Unfortunately, most business owners overlook this key point and as a result, focus on the wrong things during the deal. They think they're selling past performance, but what buyers really want is a return on their investment, which can only come from future opportunities."
In particular, Currie recommends selling when:
- You have dominant market share or a recognized position, such as price leadership.
- You have some sort of technological or competitive advantage.
- Margins are good and have the potential to get even better.
- You have something — such as infrastructure, distribution or access to a niche market — that can be leveraged by the right buyer.
- Your industry and market are in an up cycle.
What to Do Before the Sale
Before posting the "for sale" sign on the front lawn of your business, Collins and Currie strongly recommend familiarizing yourself with the following concepts:
- Recognize that the company is the product. When selling a business, the company — not its products, services or technology — represents the ultimate product. According to Currie, the notion of "company as product" means that owners must pay attention to things like operations, systems, management, markets, future opportunities, intangible competencies and relationships with customers, vendors and employees — all the elements a potential buyer will scrutinize when giving the business the once-over.
- Look at your company through the buyer's eyes. Like proud parents, most business owners tend to focus on the positive attributes of their company while overlooking any warts that may lie just beneath the surface. Buyers, on the other hand, look at everything. In addition to sales, inventory, debt load and other obvious indicators, smart buyers carefully scrutinize things like:
- The owner's salary and perks
- Existing leases and other financial obligations
- Employee benefits
- Relationships with suppliers or vendors that are based on personal rather than business relationships
- Relatives on the payroll
- Club memberships, cars, planes, etc., paid for with company assets
"These things may make running your business more enjoyable, but they significantly dilute the strength of your balance sheet," says Collins. "They also turn off potential buyers and lower the price they might be willing to pay. To get maximum value for your business, look at it from the buyer's perspective and clean up any blemishes that take the luster off your company's shine."
- Understand the buyer's motivation. Ask, "Who wants what my business has and can do more with it than me?" The answers, says Currie, can be found in your systems, processes, people, technology, teams, training, suppliers, customers, locations, geography or some combination of these. "Buyers seldom buy what sellers think they're selling," he points out. "To secure the best deal, you must know why a buyer would want you."
- Enhance the intangibles that make your business attractive. This requires familiarizing yourself with the buyer's world. Find out all you can about potential buyers' companies, where they want to go and what they need to get there. Research their industries by attending trade shows, reading journals and joining associations. The more you know about buyers' wants and needs, the more you can do to enhance the value of your business.
- Know the value of your business. According to Collins, many owners do not know how much their companies are worth. Often, they come up with a figure based on how much they feel they need to live on for the rest of their lives. Instead, Collins recommends a formal appraisal, updated on an annual basis. In particular, be sure to use valuation methods that potential buyers of your business might use. (For a brief overview of valuation methods, see the article "Creating the Exit Plan" below). Without a reasonable idea of the value of your business, you have no real starting point to begin negotiations.
How to Get the Best Price
There is no such thing as the "right" price for a business. As a seller, you want to obtain the highest price while the buyers seeks to pay as little as possible. To ensure that you get the best possible return on your investment, Collins and Currie recommend the following strategies:
- Never state a price for your company. Let the market determine the price by discreetly obtaining offers from a variety of potential buyers. Once you state a price, you will never get more than that. In most cases, you will end up with less.
- Negotiate with multiple buyers. The quickest way to erode the value of your business is to negotiate with only one buyer.
- Seek out strategic buyers. What constitutes a strategic buyer? According to Currie, it's "anyone who wants what your business has and can do more with it than you." A strategic buyer looks at your potential and your intangibles. A financial buyer focuses on past performance. A motivated strategic buyer will almost always outbid a financial buyer.
- Use an intermediary. Intermediaries, such as business brokers and M&A specialists, offer many benefits, including:
- Experience selling companies
- Ability to find and qualify the buyers
- Ability to maintain confidentiality
- Experience playing multiple buyers against each other
- Knowing what information to release to buyers and when
"Most business owners only sell the business once; most buyers buy many times. So they have a distinct advantage," says Collins. "No matter how sophisticated you may be when it comes to running your business, selling it is a whole different ballgame. Try to do it on your own and you will very likely get taken to the cleaners."
Currie agrees. "In addition to their knowledge and experience, intermediaries also provide the critical ability to assess the deal from a third-party objective. They offer a sort of 'emotional firewall,' that keeps you from getting too caught up in the feelings that surround the deal. Granted, these kinds of professionals don't come cheap. But in most cases, you more than offset the fees by gaining a higher price for your business."
- Understand product and industry life cycles. Sell your company when both of these are on an upward growth curve. Buyers want future potential, not yesterday's track record.
- Stick with your exit plan. Your exit plan should guide every major decision. Stay focused on building the strategic value of your company and creating wealth instead of just a job. If the deal doesn't jibe with your overall exit plan, don't sign on the dotted line.


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|Kai Ahrens <ka_...@ahrens-netz.de>
|Re: [discuss] remove of binfilter module
|Tue, 14 Jun 2011 22:09:09 GMT
Hi Raphael, Am 15.06.2011 00:01, schrieb Raphael Bircher: > Hi at all > > I'm not sure if the Users are so happy with it. LibO had the same > discussion same month ago, with the result uf user protests. Switch back > to a older OOo Version is not realy a option, because it's not easy to > install two different OpenOffice.org on one mashine. > > If we will do so, we have to provide a extension as minimum. else I vote -1 Sure. As Mathias already pointed out, the code itself should be accessible within the repository. That's my understanding, too. But maintaining a 'to be done' binfilter extension would then be in the hands of those needing it so desperately. This is also my understanding. Regards Kai
|View raw message


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Nataliya Dobrynska, Pentathlon, Ukraine
Born: 29 May 1982
The chief threat to Jessica Ennis, Dobrynska is the Olympic heptathlon champion. Her strengths are her throwing arm and her stamina. If she can stay in touch through the explosive sprints, where Ennis is quicker, her ability over 800 metres will make her a genuine threat.
Carmelita Jeter, 60 metres, USA
Born: 24 November 1979
A late developer, Jeter dramatically improved her speed over 100metres in 2009, to the point where only Florence Griffith-Joyner has run faster. Her recent 60-metre time of 7.06 at the Aviva Grand Prix in Birmingham was a personal best, and many believe that Irina Privalova's 17-year-old world record of 6.92 could come under threat in Doha.
Christian Olsson, triple-jump, Sweden
Born: 25 January 190
After five separate bouts of surgery, Olsson is one of the fewsportsmen who can compete with Andrew Flintoff when it comes to scalpel scars. Won this event in 2003 and 2004, before his feet began to give out, but at 30 he is not old for the sport. Jonathan Edwards was still winning triple-jump titles at 36.
Steve Hooker, pole vault, Australia
Born: 16 July 1982
Melbourne-based Hooker stands behind only Sergei Bubka in the list ofgreat pole-vaulters, with a personal best of 6.06 to Bubka's 6.14. He is the reigning world and Olympic champion and must be one of the hottest favourites in any event.
Trey Hardee, Heptathlon, USA
Born: 7 Feb 1984
The reigning decathlon world champion, Hardee took on the mantle ofhis American stablemate Bryan Clay to set a personal best of 8790 in Berlin. He is a master of the speed events, but Leonel Suarez, the 22-year-old Cuban, is improving all the time.
Blanka Vlasic, high jump, Croatia
Born: 8 November 1983
Croatia's 6ft 4in pin-up, Vlasic cleared a new indoor personal best of 2.06 at a meeting in Arnstadt in early February. The world record of 2.08 should be in her sights in Doha.
Dayron Robles, 60m hurdles, Cuba
Born: 19 November 1986
Robles crashed out of the last world indoor championships, in Valencia two years ago, when he stopped running in the mistaken belief than a false start had been called. Even so, he came back strongly to win Olympic gold in Beijing that same year. Recently ran an impressive 7.46 seconds at the Aviva Grand Prix in Birmingham.
Priscilla Lopes-Schliep, 60m hurdles, Canada
Born: 26 August 1982
Lopes-Schliep is another hurdler with a score to settle after she fellin the first round in Valencia. Her main opposition is expected to come from America's Lolo Jones, the reigning world indoor champion, who famously suffered a similar fate just a few feet from the line in Berlin.
Yelena Isinbayeva, pole vault, Russia
Born: 3 June 1982
You can't have a much better CV than Isinbayeva, who has taken gold at the last three world indoor championships. Yet her domination of the event was broken at the outdoor world championships in Berlin, whereshe failed to register a successful attempt.
David Gillick, 400m, Ireland
Born: July 9, 1983
The 26-year-old Irishman made an explosive impact in Birmingham on February 20 when he ran 45.52, the fastest European indoor time for three years. Only one runner has gone faster in the world this year – the USA's Torrin Lawrence – and he is not expected to compete in Doha.
Watch athletics live online exclusively at telegraph.co.uk/athleticslive, with action from the IAAF indoor meeting in Lievin on March 5, before full live coverage of the World Indoor Athletics Championships on March 12, 13 and 14, in association with Aviva.


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The landlord does not accept the following in this place:
Barcelona - a city that's brimming with life around every corner and that's surely going to impress and inspire you! Being the largest metropolis on the Mediterranean coast, this wonderful city is home to many students in pursuit of their academic dreams. If you want to join this army of young minds and chase after your academic goals, then we have a great spot for you to settle into. Located in El Putxet, close to several public transport links and many cafes, stores and restaurants as well as not too far from IED Barcelona and EU Business School - International Offices, this well-located accommodation is surely going to provide you with a great and memorable student lifestyle!
This apartment has four bedrooms, a living room, a kitchen and two bathrooms. This charming property delivers all that you seek, from location to comfort.
Go big and come to your new home!
Find out more about this property's common areas in our photo gallery.
You will be sharing this property with the landlord.
Looks like no one had the time to review this place yet!
These conditions were defined by the Provider and are subject to change.
We have not verified this property yet. We are working on having it fully verified as soon as possible.
Getting Around
Your new neighbourhood
El Putxet
Path to university
At Uniplaces we make the process of booking an apartment as easy as possible for both tenant and landlord.
That's why we have our own team of professional photographers that verify our properties and pick our landlords carefully in order to save time on viewings: we think it's old-fashioned and time-consuming.
You can look at forty different apartments on our platform in the same amount of time that it takes to see a room.
The pictures you see here haven't been photoshopped, so they'll be really accurate.
If you've still got any doubts, we'll keep the money that you transfer us until 24h after your move-in date.
If something's not exactly what you saw online, we'll help you move somewhere else and return your money.
We can only give you the contact details of your landlord after your booking is accepted. But we can guarantee that Uniplaces chooses the landlords it works with very carefully.
Besides, we've had students whose favourite part was living with a local!
Landlords and host families can be great city guides, help you out with translations and even become your friends.
As soon as your booking is accepted, you're given the landlord's contact details. Then, you can discuss all details directly with them, such as when you'll be moving-in.
Students move in and out at different times, so your flatmates might change during your stay.
It's a great opportunity to make new friends!
We personally speak to students who we place in Uniplaces apartments and are sure we will find the right place for you as well!
At the bottom of the property page, you have a calendar where you can see the availability of that room/apartment, according to the landlord.
But if you're still unsure about that we're happy to help.
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There are two different types of deposits: the Holding and Security deposits.
By clicking on "Deposit" at the bottom of the offer page you will get more information about each type of deposit.
In general, the deposit is paid to cover for any potential damages that may occur during your period of stay or will be used as rent compensation in case you leave earlier than originally planned.
If no damages occur and you leave on the agreed move-out date, your deposit should be refunded by the landlord in a way you both agree on.


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Next year, we have a chance to be very good, but so does the rest of the B10. There should be 6 B10 teams in the pre-season top 40, and I'm guessing that us, Michigan and Iowa will be predicted 7-9 coming in. But we can do better - if we get positive answers to some of the following questions.
1. Will our playmakers be able to shoot?
Specifically, I have in mind T.J. Parker, Mohamed Hachad and Mike Thompson.2. Will we have a post threat next year?
MT and VV are the two main candidates, or maybe Mo.I think those are the two main issues, but here are some things that could make us better as well.
3. How will Mo's (and VV's) decision making be?Comments? [Links to the Purple Basketball Board]
Well documented - and I'm more concerned about that than the shooting. In fact, VV could use to cut down on the mistakes as well. Having your two best playmakers be the two worst culprits for mistakes is a recipe for bad losses.4. Will VV be able to finish around the basket?
Self-explanatory, and tied into #2. He could be all B10 if he can.5. What impact will the 1st year [new] players have?
Sterling Williams and Bernard Cote could really help, but who knows? In fact, if Brandon Lee gets back on track he could make things happen as well.
Beat 'em All!
|Talk about it on the Purple Boards
[Click Here]


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Health insurance with dental is available for kids 0-18 through Florida KidCare.
Most families pay $15 or $20 per month or less. Eligibility is based on household size and income.
In Jacksonville, Dial 211 for information or application assistance.
For information about the Summer Meals Program, call (904) 630-6430.
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text:
Like many of you I keep seeing and hearing statements like "secure firewall friendly connection" on numerous products.
Just think about what this really means – the firewall has little chance of doing anything useful as traffic is being tunnelled through in encrypted form. This is not necessarily a problem but it's important to be aware that your threat profile changes as a result.
It means that application traffic will be transmitted through the firewall via a protocol that's highly likely to be allowed through – namely HTTP using SSL to encrypt the data (and authenticate the server to the client) hence HTTPS.
A small number of firewalls such as Microsoft's Internet Security and Acceleration Server (ISA) can be configured to intercept INBOUND HTTPS and inspect the content to ensure it complies with policy. Most firewalls still limit their inspection to just the packet headers and ignore the content. Inbound inspection is achieved by configuring ISA to take on the identity of the web server via it's certificate and associated private key. Optionally ISA can be configured to re-establish the SSL tunnel between itself and the internal webserver.
The OUTBOUND scenario is rather different. If someone in your organisation establishes an outbound connection to a remote webserver via SSL (HTTPS) then the firewall can't inspect it without breaking the protocol as the required private key for the target webserver is not available.
As you may be aware many vendors (including Microsoft) advocate tunnelling as it's often an effective way to easily deploy client server applications (like Outlook and Exchange) either side of firewall infrastructure.
The term "secure firewall friendly" used in this way is almost an oxymoron. Of course using SSL in the prescribed manner gives authentication of the server to the client and encryption of the data in transit – both potentially good things – but it generally negates the effectiveness of your firewall.
Note: In days of old such applications used to use their own ports and required the firewall administrator to open up holes accordingly for each giving granularity. Firewall admins generally became viewed as obstinate people who resisted change "in the name of security" and therefore applications started tunnelling over existing ports.


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Contrasted patterns of selective pressure in three recent paralogous gene pairs in the
Gene duplications are a molecular mechanism potentially mediating generation of functional novelty. However, the probabilities of maintenance and functional divergence of duplicated genes are shaped by selective pressures acting on gene copies immediately after the duplication event. The ratio of non-synonymous to synonymous substitution rates in protein-coding sequences provides a means to investigate selective pressures based on genic sequences. Three molecular signatures can reveal early stages of functional divergence between gene copies: change in the level of purifying selection between paralogous genes, occurrence of positive selection, and transient relaxed purifying selection following gene duplication. We studied three pairs of genes that are known to be involved in an interaction with symbiotic bacteria and were recently duplicated in the history of the
Selective histories revealed by these three signatures of molecular differentiation were found to be markedly different between each pair of paralogs. We found sites under positive selection in the
This study illustrates the variety of selective pressures undergone by duplicated genes and the effect of age of the duplication. We found that relaxation of selective constraints immediately after duplication might promote adaptive divergence.
Gene duplications have long been hypothesized to be drivers of genome and gene function evolution
Starting with Ohno, a range of models has been proposed to predict the fates of paralogous gene pairs resulting from duplications. These models can be categorized by their assumptions: they can be either neutral or involving natural selection, and can consider the early stage of duplication,
Immediately after the gene duplication event, the two copies are assumed to be identical and therefore functionally redundant. At this stage, there should be no selective pressure against any loss-of-function mutation affecting either copy. As a result, it is believed that most instances of gene duplications will eventually result in the loss of one of the copies (pseudogenization or nonfunctionalization). However, the relaxation of purifying selection (due to the initial redundancy) may allow some amount of divergence and occasionally can let one copy acquire a new function and be subsequently maintained by natural selection (neofunctionalization). This scenario is essential for the creative role of duplication envisioned by Ohno
Functional analyses have been performed in order to determine the relative importance or the interaction between these different models. The occurrence and the characteristics of functional divergence of paralogous genes can be addressed either through the regulatory or protein-coding sequence angle.
Whole-genome expression profiles revealed divergent expression patterns between paralogous gene pairs, providing indirect evidence for subfonctionalization and/or neofunctionalization
Beside differences in gene expression, rates of molecular evolution can be used to qualify the constraints experienced by genes. In particular, contrasting the rate of protein-changing (non-synonymous) substitution (dN) and the rate of silent (synonymous) substitution (dS) at the nucleotide level allows qualifying the type of selection acting on individual gene copies after a duplication event. The intensity of purifying selection is often estimated through the ratio ω = dN/dS. Values of ω < 1 are interpreted as evidence for purifying selection (the lower ω, the stronger purifying selection). Following pseudogenization, ω = 1 is expected (no constraint). Last, amino acid sites exhibiting ω > 1 are likely directly targeted by positive selection. As an example, the evolutionary fate of ten genes recently duplicated by retrotransposition in mice was studied by contrasting synonymous and non-synonymous rates
We investigated rates of molecular evolution of three duplicated gene pairs in the genus
Mutualistic host-symbiont interactions present the interest of combining several features we can expect will promot fast evolution. Mutualisms are often based on nutrient exchanges and involve strong selective pressures, since both costs and benefits are important. The interaction with a biotic partner can cause shifting selective optima, especially if there are conflicts of interest. Finally, in contrast with host-pathogen interactions, mutualisms can involve the evolution of novel structures by both partners. The legume-rhizobium symbiosis evolved relatively recently, around 60 million years ago, culminating with the emergence of a specific organ, the root nodule
Rates of molecular evolution of paralogous gene copies (hereafter paralogs) should be studied preferably in a variety of species to have enough power to inner substitution rates. Moreover paralogs should be characterized in a set of extant species that have diverged after the ancestral gene duplication. In spite the growing availability of full genome sequences, plant model species are usually not related enough to allow for analysis of divergence at the nucleotide level. In the case of the Fabaceae family, three species have been sequence (
Depending of the gene, a successful amplification was obtained for a total of 10 to 17 species. The resulting sequence alignments had a length of 729 bp for
Additional file 1
Sequencing results. Table in PDF format presenting sequencing results for the five genes on the 17 species and GenBank accession numbers. Lengths are indicated in base pairs. The percentage that each sequence represents relative to the complete alignment is indicated in brackets when less than 100%. "
Click here for file
Phylogeny of
The phylogeny of
Figure 1
Phylogenetic trees for
Phylogenetic trees for
The species phylogeny deduced from the data was not completely congruent between different paralogs and with the species phylogeny described in the literature
Analysis of selective pressures along trees: testing for an "age" and a "paralog" effect
Comparing models with different constraints on the value of ω among branches of the tree allows testing evolutionary hypotheses (Figure
Figure 2
Schematic representation of the codon models used
Schematic representation of the codon models used. (a) Models allowing dN/dS variation along lineages. Arrows indicate the questions addressed by the comparison between models (in (a), the arrows correspond to hierachical relationships). (b) Models allowing dN/dS variation along the gene. In M8A, ω follows a β distribution discretized into 10 categories of similar frequency (0 < ω1–10 < 1) and an additional category of ω is fixed at 1 (ωad = 1, accounting for neutral sites); M8 differs from M8A only by the additional category of ω which is constraint to be superior to 1 (ωad > 1), to account for sites under positive selection. (c) Phylogenetic trees harbours two clades, one for each paralog (the outgroup is not represented here). Models are either specifying identical categories of dN/dS in both clades (M3), or allowing one category to take a different value in each clade (MD).
Interestingly, the three paralogs pairs exhibited contrasted results. The
For the second paralog pair,
For the third and most recent pair of paralogous genes,
Analysis of selective pressures along genes: testing for positive selection
In order to investigate how ω varies along genes and in particular if positive selection signatures occurred we used models in which ω is allowed to vary among sites on each gene (Figure
We compared the fit of models M8A and M8. The likelihood ratio test of M8 against M8A is a conservative test for positive selection, since the M8A model can account for an excess of neutral sites. No sites under positive selection were found for
Selective pressures along branches and sites of each paralog: testing for a "paralog" effect
In the third model we used, the clade model MD
For the three paralogous gene pairs studied, models MD for which one class of ω is allowed to differ between paralogous gene clades were significantly better than null models M3 in which no variation between clades is allowed (Table
In this paper we examined patterns of molecular evolution of three paralogous gene pairs, in order to detect signatures of post-duplication functional divergence. We chose a time scale that allows analysing patterns of natural selection by examining patterns of nucleotide substitution of protein-coding sequences. With that aim, we focused on three sets of paralogs from the
Models describing the evolutionary fate of duplicated genes once the duplication is fixed in the species suppose different forms of selective pressures
For all three studied paralogous gene pairs, the two copies exhibit different regimes of selection. This result suggests that these paralogous gene pairs have undergone at least some functional differentiation. Three different tests were used to qualify selective pressures governing the paralogs. The first one contrasted the average ω between paralog clades of the phylogeny and yielded significant differences only for
We observed that the
Functional redundancy generated by multiple copies also implies periods of relaxed selection pressures, except if duplication itself is advantageous as it is the case, for instance, for a positive dose effect of copy number
We detected sites under positive selection in
According to the clade models, the paralogs
The models contrasting ω in different branches allowed testing transient relaxation of purifying selection predicted to occur immediately after duplication. A significant increase of ω was detected in basal branches of the
This study illustrates the multiplicity of mechanisms governing the evolutionary fate of duplicated genes and, in particular, the relative age of the duplication. Analysis of nucleotide substitution rates in gene coding sequence can discriminate between qualitative phenomenon (occurrence of positive selection) or quantitative differences (levels of ω between clades and its variation among branch and sites). Further studies of the factors governing evolution of duplicated genes will benefit from taking into account features of the evolution of gene families involving successive rounds of duplications.
Plant material
One accession was selected in sixteen diploid species of the
Additional file 2
List of species used. Table in PDF format with list of sample used, germplasm accession number, life history, geographical area and ploidy level.
Click here for file
Selection of duplicated genes
Genes were chosen on the basis of the
Second, we chose family of auxin efflux carrier,
To amplify specifically the coding region of each paralogous gene, we defined specific and non-specific primers. Non-specific primers were defined using the common sequence of both paralogous gene for each pair (
Additional file 3
List of primers used. Table in PDF format with names and sequences of primers used for amplification and sequencing.
Click here for file
Additional file 4
Schematic representation of genes and primers positions. Figure in PDF format with schematic representation of the intron/exon structure of the 5 sequenced genes on
Click here for file
Most sequences were obtained from genomic DNA, except for
Phylogenetic analysis
Maximum-likelihood phylogenetic trees were inferred using the PHYML program
Additional file 5
Lax gene Nucleotide alignment. Nucleotide alignment of
Click here for file
Additional file 6
Click here for file
Additional file 7
Click here for file
Additional file 8
Pg gene amino acid alignment. Amino acid alignment of
Click here for file
Variation in substitution rates was analyzed using codon substitution models where the parameter ω is defined as the ratio of non-synonymous (dN) to synonymous (dS) substitution rates
Next, two models allowing for variation of ω among sites, but not among branches of the phylogenetic tree, and that are designed specifically to detect positive selection were used (Figure
The last two models are so called branch-site models that are combining variation of omega both among amino acid positions of the alignment and between different clades of the phylogeny, in our case each paralog clade (Figure
Maximum likelihood estimation of all model parameters was performed using the codeml software of the PAML package
Pg: Polygalacturonase; Lax: Like
Competing interests
The authors do not have any kind of financial or non-financial competing interest to declare in relation to this manuscript.
Authors' contributions
NC and JR conceived and designed research, JHH, IH and AW acquired data, JHH and NC processed data, JHH, NC and SDM analyzed data and NC, JHH, JR, SDM and TB wrote the paper. All authors read and approved the final manuscript.
Sequencing was funded by an "AIP Genomes" grant by INRA. This work was also supported by ARCAD, a flagship project of the Agropolis Foundation.
The origins of genome architecture
The evolutionary fate and consequences of duplicate genes
Extensive duplication and reshuffing in the Arabidopsis genome
Functional divergence of duplicated genes formed by polyploidy during Arabidopsis evolution
Widespread genome duplications throughout the history of flowering plants
The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla
Genome sequence of the palaeopolyploid soybean
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
Genome evolution in polyploids
The evolution of gene duplications: classifying and distinguishing between models
Preservation of duplicate genes by complementary, degenerative mutations
The evolutionary dynamics of plant duplicate genes
Role of duplicate genes in genetic robustness against null mutations
Rapid subfunctionalization accompanied by prolonged and substantial neofunctionalization in duplicate gene evolution
Comparable rates of gene loss and functional divergence after genome duplications early in vertebrate evolution
Expression divergence between duplicate genes
Evolution of Duplicate Gene Expression in Polyploid and Hybrid Plants
Duplicate gene evolution and expression in the wake of vertebrate allopolyploidization
Reciprocal silencing, transcriptional bias and functional divergence of homeologs in polyploid cotton (gossypium)
Escape from adaptive conflict after duplication in an anthocyanin pathway gene
Gene duplication and the adaptive evolution of a classic genetic switch
The evolutionary fate of recently duplicated retrogenes in mice
Nonrandom divergence of gene expression following gene and genome duplications in the flowering plant Arabidopsis thaliana
Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis
From pollen tubes to infection threads: recruitment of Medicago floral pectic genes for symbiosis
Evolving ideas of legume evolution and diversity: a taxonomic perspective on the occurrence of nodulation
The Medicago genome provides insight into the evolution of rhizobial symbioses
Evolutionary rates analysis of Leguminosae implicates a rapid diversification of lineages during the tertiary
Ribosomal External and Internal Transcribed Spacers: Combined Use in the Phylogenetic Analysis of Medicago (Leguminosae)
Phylogeny and character evolution in Medicago (Leguminosae): Evidence from analyses of plastid trnK/matK and nuclear GA3ox1 sequences
The reticulate history of Medicago (Fabaceae)
A maximum likelihood method for detecting functional divergence at individual codon sites, with application to gene family evolution
The effect of gene conversion on the divergence between duplicated genes
Patterns of expansion and expression divergence in the plant polygalacturonase gene family
Point mutations with positive selection were a major force during the evolution of a receptor-kinase resistance gene family of rice
Excess non-synonymous substitutions suggest that positive selection episodes occurred during the evolution of DNA-binding domains in the Arabidopsis R2R3-MYB gene family
Molecular evolution of the CPP-like gene family in plants: insights from comparative genomics of Arabidopsis and rice
The PIN and LAX families of auxin transport genes in Medicago truncatula
Root nodulation: a paradigm for how plant-microbe symbiosis influences host developmental pathways
Molecular evolution and positive selection of the symbiotic gene NORK in Medicago truncatula
The Staden sequence analysis package
Artemis: sequence visualization and annotation
A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood
A codon-based model of nucleotide substitution for protein-coding DNA sequences
Likelihood ratio tests for detecting positive selection and application to primate lysozyme evolution
Codon-substitution models for heterogeneous selection pressure at amino acid sites
PAML: a program package for phylogenetic analysis by maximum likelihood


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Stylophora pistillata, a reef coral in the Northern Red Sea, thrived in simulated global-warming conditions.
Discoverer of Lysosomes Dies
May 8, 2013|
Christian de Duve chose to be euthanized at home in Belgium at age 95.
May 8, 2013|
Christian de Duve chose to be euthanized at home in Belgium at age 95.
May 5, 2013|
As telomeres shorten with age, genes as far as 1,000 kilobases away could be affected, including one responsible for an inherited muscle disease.
May 2, 2013|
Researchers use a protein-lipid complex found in human breast milk to increase the activity of otherwise-ineffective antibiotics against drug-resistant pathogens.
May 2, 2013|
Hybrid viruses derived from an H5N1 bird flu strain can infect guinea pigs through the air.
May 1, 2013|
Research Associate, Perelman School of Medicine, University of Pennsylvania. Age: 27
May 1, 2013|
By varying the size of their steps, dynein motor proteins work effectively as teams to carry heavy loads around the cell.
May 1, 2013|
Sorting out T-cell functional and phenotypic heterogeneity depends on studying single cells.
May 1, 2013|
Viral infections of the central nervous system may trigger cytokines that induce seizures.
May 1, 2013|
Researchers are using modern experimental tools to probe the mysterious molecular pathways that lead to premature labor and birth.
April 22, 2013|
Rodents and fruit flies appear to be able to sense nutrients even when they can't taste the food they're eating. Now, researchers are trying to figure out how.


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Christine Kuo gained a lot of weight after being diagnosed with hyperthyroidism and was even called "Fat Christine" by some netizens.
However, she recently embarked on a slimming regime and it has proved to be effective.
Read also: Christine Kuo loses weight and is on her way to regaining her slim figure
She has been posting several photos of herself on her Instagram showing her lithe body.
Netizens praised her on congratulated her on slimming down and hope she will return to her fit body figure soon.
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Nothing beats the sublime beauty of a desert lake, its azure waters shimmering dreamily against the golden hues around it, green grasses waving invitingly in the breeze, beckoning to thirsty animals and weary travelers.
Twin Lakes Reservoir, Utah
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Check out local things to do such as local golfing (Old Mill Golf Course, Creekside Course and Sagebrush Course), water sports (POCO Loco Swim Shop, REI - Recreational Equipment- Inc. and Golden Rentals), hiking (The Sheep Trail, Notch Mountain Trail and Lofty Lake Trail).
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Growing medicinal plants are a great way to ensure garden sustainability and more notably, have access to natural medicine when you need it most. When I introduced more herbs in my garden, I noticed it had a profound impact on the vegetables and fruits I was growing. It also encouraged beneficial insects and birds to visit my garden and this helped cut down on plants being eaten.
Because of this observation, I changed my focus from solely growing to eat and, instead, worked to create a welcoming growing environment. Not only were my plants healthier, but I had access to natural herbs to use for making extracts and poultices. The following are reasons I feel gardeners should adopt adding medicinal herbs to the garden.
(Sign up for our FREE newsletter to get the latest health advice, gardening secrets, homesteading tips and more delivered straight to your inbox!)
6 Reasons Why You Should Have a Medicinal Garden
Ready Nutrition writer and herbalist, Jeremiah Johnson has written extensively on how to cultivate a medicinal garden to use in a long-term emergency. His favorite medicinals are what he refers to as the 3 G's: garlic, ginger, and ginseng. You can read his article on the subject.
To better understand natural medicine and using herbals for health, I strongly recommend you read more on the subject. The following books come highly recommended:
Herbal Antibiotics: Natural Alternatives for Treating Drug-Resistant Bacteria," by Stephen Harrod Buhner.
Prepper's Natural Medicine: Life-Saving Herbs, Essential Oils and Natural Remedies for When There is No Doctor, by Cat Ellis (Herbal Prepper)
This is not a new gardening concept, yet is still not widely used. When you are planting your garden, consider adding a few herbs and watch the benefits grow before your eyes.
Tess Pennington is the author of The Prepper's Blueprint, a comprehensive guide that uses real-life scenarios to help you prepare for any disaster. Because a crisis rarely stops with a triggering event the aftermath can spiral, having the capacity to cripple our normal ways of life. The well-rounded, multi-layered approach outlined in the Blueprint helps you make sense of a wide array of preparedness concepts through easily digestible action items and supply lists.
Tess is also the author of the highly rated Prepper's Cookbook, which helps you to create a plan for stocking, organizing and maintaining a proper emergency food supply and includes over 300 recipes for nutritious, delicious, life-saving meals.
Visit her web site at ReadyNutrition.com for an extensive compilation of free information on preparedness, homesteading, and healthy living.
This information has been made available by Ready Nutrition
Trump Turned To NATO, Said One Thing, and Then Immediately All Hell Broke Loose…
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Mark Twain, "On the Damned Human Race"
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These Flat-Pack Turtlebots Will Crawl Across Minefields for Safety's Sake


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Turn its frown upside down: fill it with gifts
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This deliciously thick layer of cinnamon swirl sponge is topped with our creamy vanilla cheesecake and covered in a rich cinnamon streusel crumble, all baked until perfectly golden brown and finished with a classic white icing drizzle. A classic winter dessert with a unique take.Serves:12 Size: 8inch Weight: 1.385kg
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CANADA STOCKS-TSX set to open lower as commodities hit
TORONTO Dec 19 (Reuters) - Toronto's main stock index is poised to open lower on Friday as the resource-laden market may be hit by sliding oil and gold prices and as fertilizer giant Potash Corp of Saskatchewan (POT.TO: Quote) cut its full-year earnings outlook.
On Thursday, the S&P/TSX composite index .GSPTSE closed down 298.76 points, or 3.42 percent, to 8,425.35, as tumbling oil prices hit energy issues while materials and financial stocks felt the sting of deepening worries about the health of the global economy.
Here is some of the news that may affect the market:
Canada's annual inflation rate dropped to 2.0 percent in November from 2.6 percent in October as lower gas prices offset an increase in food and shelter costs, Statistics Canada said on Friday. [ID:nN19431958]
POTASH (POT.TO: Quote)
Potash Corp of Saskatchewan Inc, the world's biggest fertilizer company, cut its outlook for full-year earnings by 10 percent, blaming the weak world economy. [ID:nN18416174]
Research In Motion RIMM.O delivered a quarterly profit in line with forecasts and a rosier-than-expected outlook that reflects strong holiday sales of its BlackBerry smartphones even as the global economy slows. [ID:nN18411402] Continued...


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So, for those who are interested in, or have been invited to join the writing of the story "Code Name: Snow White". Characters have not been claimed, the plot has yet to be hashed out, nor has it been decided if this will be a well planned group effort, or more of a "tag your it" kind of deal. I'm open to both, but I think I kind of like "tag your it" best. I love surprises. Okay people, what are you thinking?
what is this story about? can just anyone join it?
So i started a story and really hoped someone would jump on a few months ago. I got desperate and even started continuing some of the branches myself. This story is about a team of multi-racial, supernatural entities who fight supernatural crime in order to keep the world balanced, by any means unnecessary. So far we have steamy relationships, vampires, angels, human, demons, drugs, in-laws, assassinations, explosions and VERY big guns. I'm willing to drop my added branches if someone wants to take over a character. This would be so much more fun with other people! This is designed for adults as the content is mature. I'm a teacher and NOT willing to risk my career over a mature collab with a kid, regardless of how talented you are. It's inappropriate, sorry! Anyway, somebody hit me up! Here is a link to the intro chapter...
http://www.protagonize.com/story/code-name-snow-white - @SummerDreams
http://www.protagonize.com/story/code-name-snow-white - @SummerDreams
I like 'tag you're it' a whole bunch, but its ultimately your story so you choose
Essentially we can hash out some basic plot points here and work the rest out on the fly.
So I suppose it's best if Deep V lays out the skeleton plot for us.
Essentially we can hash out some basic plot points here and work the rest out on the fly.
So I suppose it's best if Deep V lays out the skeleton plot for us.
Okay folks, after so much inactivity, I decided to close the story. Thanks anyway!


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Beginnings...Ends...Die...Just Stuff Nobody Cares About Enough To Read
Don't bother critiquing, just let me think out loud for a while. Comment, if you like, but no critiquing, please. I'm always open to a casual comment.
I always hate beginnings. Endings give some sort of closure, endings are definite, like when somebody dies and the body is found. Beginnings are like when somebody is abducted. No closure, just one lifelong beginning, the middle of a story going on forever until everything just runs down like a machine and we die.
Endings. My favourite part of a book, if they're done well. Closure. Make me cry until the ink blurs, make me sick with shock, make me angry. Don't just END.
Anticlimax is the equivalent of just dying, after doing nothing to be remembered by. I don't even know why I write this stuff down.


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Indianapolis Colts Owner Pleads Guilty To OWI & Suspended By NFL [Update]
Indianapolis Colts Owner Jim Irsay will avoid jail time after getting caught driving a little wild as he tried "to find his house."
In March, the outspoken owner was caught with $29,029 in cash and a lot of prescription drugs as he was pulled over after driving "erratically."
After originally pleading not guilty to two counts of OWI – a misdemeanor, Irsay has decided to change his tune and plead guilty but will have to serve one year of probation which includes random drug testing according to Indianapolis Star's Mark Alesia.
If he gets another OWI in the next five years the charge will be changed to a felony.
The NFL suspended Irsay for six games and levied a fine of $500,000 for his role in the arrest. The suspension will begin on Wednesday.


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By RYAN TOLMICH
Exactly one week after combining for six scores, Toronto FC and the Houston Dynamo brought the goals out of each other yet again.
The two Eastern Conference sides split four first-half finishes, drawing 2-2 Saturday at BBVA Compass Stadium.
Dynamo forward Will Bruin started the scoring in the 11th minute by taking advantage of an errant backpass from TFC defender Bradley Orr to a charging Joe Bendik. Orr and Bendik's miscommunication left Bruin with an open net, and the Dynamo forward took advantage of the error to make the score 1-0.
TFC equalized just seven minutes later via Gilberto, who scored just seconds after Dynamo midfielder Brad Davis' shot was saved on the other end. TFC's methodical counter saw the ball move through the Dynamo defense before finding its way to Gilberto, who took a heavy touch in the box before firing past Dynamo goalkeeper Tally Hall.
The hosts restored their lead just eight minutes later, as Giles Barnes fired past from distance in the 26th minute to give the Dynamo a 2-1 lead. Barnes took a pass from Ricardo Clark and into space before shooting from 25 yards and past a cheating Bendik.
Despite a 28th minute Michael Bradley penalty miss, TFC's Dominic Oduro netted the game's fourth and final goal in extra time after a spectacular run and cross from TFC's Luke Moore. Moore sprinted his way towards the backline past Dynamo defender AJ Cochran before playing a cross through the Dynamo box, past Hall and into the path of Oduro, who tapped in to even things at two, where things remained until full time.
TFC will now look towards Wednesday's friendly with Tottenham Hotspur, while the Impact return to action next Saturday when they take on the Columbus Crew.
Here are the match highlights:
The fact that the Dynamo were gifted a goal and a penalty miss and they still couldn't win is unreal. But let's just pretend we don't need to strengthen the squad during the transfer window.
Just read this and I'm not feeling better. Looks like the Dynamo are stuck in MLS 2.0 only they are also unwilling to buy players in addition to developing them.
There have been a handful of distinct eras in MLS history. We tend to break it down to "MLS 1.0" and "MLS 2.0," and every week there's another benchmark (this column's new favorite buzzword) that has people declaring "MLS 3.0 is here!"
But that whole brush is too broad. If you were around in the Bad Old Days you'd know that MLS 1.0 consisted of a bunch of smaller eras. Here are a few:
"Geez, these stadiums are full" (1996)
"Geez, these stadiums are empty" (1997-2001)
"Oh crap, we're contracting" (2001-2002)
"Name a franchise after a Mexican team? I guess that could work" (2004-2005)
And there have been similar dividing lines in the days of MLS 2.0 – Toronto fans actually invented MLS support before Seattle fans invented MLS support, which happened just before Portland invented MLS support. Designated Players like Beckham, Blanco, Angel and Donovan paved the way for Designated Players like Henry, Keane, Valeri and Bradley. Tristan Bowen paved the way for DeAndre Yedlin, Diego Fagundez and Gyasi Zardes.
More to the point, Omar Gonzalez's contract extension last summer and Osvaldo Alonso's last winter paved the way for what we saw on Saturday when Sporting KC announced they'd inked Matt Besler and Graham Zusi to DP contracts. Taken all together, does that mean this is now MLS 3.0?
I'd submit that it does. Smart teams – and there's no denying that LA, Seattle and KC are three of the smartest in the league – are changing both the business model (organic, locally sourced soccer players are a wise investment) and the narrative (hey, our guys can play!).
Where's that leave the rest of MLS?
Right now, the answer is "trying to keep up." Parity is still 100 percent real, but we're starting to see a bit of talent stratification based upon developing talent from within.
That's the essence of MLS 3.0. Building players is better than buying them, and the teams with the patience and structure to do the former are about to separate themselves in a big way.
Aww. Don't be sad.
The Imperative Voice
I do think the Dynamo struggled to keep up in their two recent finals appearances against a team that used the 3 DPs well (LA). However, last year's final saw two sides that are good "play for the unit" teams — like Houston.
Houston's lack of total competitiveness this season has to do with personnel decisionmaking within the "unit" concept. The striking is mediocre and the defense is poor, and that's about the people playing there. If they revamped their need positions, they would become competitive, at which point then the concern is whether we are willing to spend for the caliber of players the elite are willing to field, or to roll the dice and pray we make a final against a SKC type balanced side and not an LA type team with high level attacking talent.
I think Houston is poor at developing its own talent which makes every signing massive. However SKC is generally a mix of foreign signings and college players — Palmer-Brown is a rarely used exception — so I don't buy them "making their own" either. Making your own is not yet something MLS does well.
I believe Houston has their hands tied with their salary cap. I wouldn't expect any big changes until the off season. I think Hall and Ashe will be used as trade bait to redo the entire back line and clear up some $$$$. Don't see Lopez, Barnes, Davis, Garcia going anywhere for the time being. We always trade away our draft picks, if we have any left maybe they will be used for a forward.
Gill King
More MB mediocrity….
Dynamo have failed at their DP signings. Current one is Alexander López, 22 year old Honduran who has shown nothing and can't break the lineup.
Boniek is a DP but he does not deserve it. His is just OK.
I don't know if Davis is a DP, he does make the money.
They signed a Mexican player a couple years back, Luis Angel Landin, to a DP contract. He gained 30 lbs between the signing and showing up to Houston. Never did a single thing for the Dynamo.
The Dynamo do not have all of the players needed to fill out a competitive roster. The club knows this, and has known for several years. I can only guess at why they do not fix their issues. The Dynamo have a good base to build from in the midfield, with Davis/Barnes/Boniek/Rico. But that's it.
That Giles Barnes though…Class.


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Deals of the day -- mergers and acquisitions
Nov 7 (Reuters) - The following bids, mergers, acquisitions and disposals were reported by 2130 GMT on Wednesday:
** French communications conglomerate Vivendi SA is looking to raise at least 7 billion euros ($8.9 billion) from the sale of Brazilian unit GVT and has attracted expressions of interest from at least four bidders, said two sources with direct knowledge of the situation.
U.S. satellite provider DirecTV said it is in the early stages of evaluating a potential acquisition of GVT.
** HSBC is nearing the sale of more than $6 billion of U.S. mortgages and other personal lending as part of its accelerated run-down of its troubled U.S. loans book.
** Apollo Global Management LLC is in exclusive talks to buy the education unit of McGraw-Hill Companies Inc , a person familiar with the matter said, in a deal that could be worth about $3 billion. Continued...


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Basseterre (St Kitts), July 15: All-rounder Ravindra Jadeja has said Team India is focused on winning Test series overseas and change the way people think about side's performances abroad.
India, under new head coach Anil Kumble, are in the Caribbean to play 4 Test matches. Jadeja, who is part of the 17-man squad, had a good outing with the ball on the opening day of a 3-day warm-up game against West Indies Cricket Board (WICB) President's XI. He claimed 3 wickets yesterday (July 14). First Test starts in Antigua on July 21.
Jadeja, Ravichandran Ashwin and Amit Mishra impressed with the ball as India restricted WICB President's XI to 180 all out. In reply, India were 93/3 at close of play on the first day on Thursday.
Speaking after the day's play, Jadeja admitted that people don't remember India's wins at home and what they care was victories outside of the country.
"Nowadays, all people care about is how much you have won overseas in Tests and ODIs. So our focus is on that and we want to win here in the West Indies," Jadeja said.
"Everyone says that we win easily in India, so we want to change that thinking. We are a young side and each one of us is eager to win in overseas conditions. Fitness and energy is good in this side and the preparation has been good. We are looking forward to winning this Test series in the West Indies," he added.
The left-hander, who has played 16 Tests, said he was happy to be back in the Test squad.
"It feels good to be playing in the longer format after a long time. You have to be match and also fit for bowling, and I have been working on it. You have to be fit enough to be able to bowl 15-20 overs in a day.
"The way I bowled today felt like I am building towards it and getting back to my full fitness. The wicket was turning slow but there was good bounce available for us. So I was trying to bowl as per my line and length," the 27-year-old said.
According to Jadeja, the conditions in the Caribbean are similar to India. "Mentally, we have to be prepared about hot and humid conditions here. But they are similar to the ones we experience when playing in the Indian summer. So our focus is on thinking about match situations and match conditions, for example when there are long partnerships.
"We have bowled as a unit today and we haven't given away too many runs, which has been our main focus. As a bowling attack, our aim is to bowl three consecutive maiden overs and put pressure on the batsmen," he added.
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