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Modeling the evolution dynamics of exon-intron structure with a general random fragmentation process
Theories and models
Most eukaryotic genes are interrupted by spliceosomal introns. The evolution of exon-intron structure remains mysterious despite rapid advance in genome sequencing technique. In this work, a novel approach is taken based on the assumptions that the evolution of exon-intron structure is a stochastic process, and that the characteristics of this process can be understood by examining its historical outcome, the present-day size distribution of internal translated exons (exon). Through the combination of simulation and modeling the size distribution of exons in different species, we propose a general random fragmentation process (GRFP) to characterize the evolution dynamics of exon-intron structure. This model accurately predicts the probability that an exon will be split by a new intron and the distribution of novel insertions along the length of the exon.
As the first observation from this model, we show that the chance for an exon to obtain an intron is proportional to its size to the 3rd power. We also show that such size dependence is nearly constant across gene, with the exception of the exons adjacent to the 5′ UTR. As the second conclusion from the model, we show that intron insertion loci follow a normal distribution with a mean of 0.5 (center of the exon) and a standard deviation of 0.11. Finally, we show that intron insertions within a gene are independent of each other for vertebrates, but are more negatively correlated for non-vertebrate. We use simulation to demonstrate that the negative correlation might result from significant intron loss during evolution, which could be explained by selection against multi-intron genes in these organisms.
The GRFP model suggests that intron gain is dynamic with a higher chance for longer exons; introns are inserted into exons randomly with the highest probability at the center of the exon. GRFP estimates that there are 78 introns in every 10 kb coding sequences for vertebrate genomes, agreeing with empirical observations. GRFP also estimates that there are significant intron losses in the evolution of non-vertebrate genomes, with extreme cases of around 57% intron loss in
Most eukaryotic genes contain spliceosomal introns, which are removed from mRNA after transcription by the RNA splicing apparatus. The biological origins of introns are uncertain. Since the discovery of introns, there has been significant debate as to whether introns in modern-day organisms were inherited from a common, ancient ancestor, the intron-early hypothesis
One way to understand the process is to examine the size distribution of internal translated exons, referring to exons that are fully translated and referred to as
In later work, Ryabov and Gribskov
On the other hand, Tenchov and Yanev
One assumption made in the exon size based approaches
In this work, we aim to revisit these competing hypotheses by addressing the following open questions: Do longer exons have an increased chance of gaining a new intron? For intron gain events, will the intron be inserted into exon randomly or at some proto-splice sites? Is there an intron gain/loss bias? Are intron insertion events independent of each other? Is there a common mechanism to explain intron gain/loss in different species? In order to answer these and other related questions, we propose a General Random Fragmentation Process (GRFP) to characterize the evolution dynamics of exon-intron structures. The parameters of GRFP are determined by combining simulation and analysis of real genomic data.
GRFP model
The model of GRFP is motivated by generalizing both Kolmogoroff fractioning process and the uniform random fragmentation process. In GRFP, the probability for an exon to split (gaining an intron) is assumed to be exponentially proportional to the length of the
The model of GRFP, illustrated in Figure
Figure 1
Demonstration of GRFP on splitting a long exon with initial size
Demonstration of GRFP on splitting a long exon with initial size
1. Given a set of
2. Within
3. Intron gains are independent of each other.
Simulation testing
We start each simulation with a long exon. The diagram in Figure
For simulations, sequence of pseudorandom number is obtained using the
We evaluate the properties of GRFP using three simulation experiments. In each, we simulate a set of ordered fragments and quantify their statistical characters given different parameters. The three sets of quantifications listed below are used to justify the three assumptions of GRFP respectively for both simulated fragments and real exons.
1. Mean and standard deviation of the size distribution by fitting it with lognormal distribution (equation (3)) or Weibull distribution (equation (4)):
Where z=E/λ,
2. Mean and standard deviation of the insertion ratio defined below:
3. Correlation between
In the first experiment, we examine the relationship between GRFP parameters and the size distribution of the simulated fragments. With fixed
In the second experiment, we examine the relationship between real
In the third experiment, we examined the effects of intron loss on the statistical characters of resulted fragments. By introducing various percentages of intron loss after intron gain, we evaluate how
Empirical data analysis
In this study, we obtained the cDNA sequences of 14 species (
For testing the first assumption of GRFP, we fitted both Weibull and normal distribution to the size distribution of vertebrate exons (logarithm scale). We also grouped exons by positions for testing position bias of intron gain/loss. For the second assumption, we fitted a normal distribution to
Empirical data analysis
Statistical counts of empirical data
Statistical counts of the extracted data are shown in Table
In this study, we ignored non-internal translated exons considering the rate of indels (a type of mutations affecting exon size distribution) is significantly lower in the coding region than the non-coding region
Size distribution of exons
Figure 2
Size distributions of vertebrate exons fitting with normal distribution
Size distributions of vertebrate exons fitting with normal distribution. The histograms of exons are fitted with a Weibull function (solid line) and normal function (dashed line).
Additional file 1: Figure S1
has the size distribution of non-vertebrate exons. Figure S2 has the size distributions of
Click here for file
In order to assess whether the size distribution of vertebrate exons is position-dependent, we grouped their exons from all protein coding genes according to their positions relative to 5′ UTRs/3′ UTRs. For the five well annotated vertebrates, the standard deviations (
Figure 3
Fitted standard deviation (
Fitted standard deviation (
These observations suggest that the size distribution of vertebrate exons could be properly fit with either Weibull or normal distribution. The Weibull distribution gives a better fit to both left and right tails (e.g., Additional file
Distribution of insertion ratio
For every gene of the selected species, we calculated the insertion ratio
Figure 4
Genome wide distribution of
Genome wide distribution of
Another interesting observation in Figure
The normal function fitted in Figure
Correlation between insertion ratios
The correlations calculated using equation (6) are shown in Figure
Figure 5
Correlation of insertion ratios for different species
Correlation of insertion ratios for different species. The correlation between
The key observation in Figure
In summary, analysis of empirical data reveals three significant differences between vertebrate and non-vertebrate genomes. First, a mixture of two normal functions gives a better fit to the size distribution of non-vertebrate exons, instead of one normal function for that of vertebrate exons; Second, the insertion ratio of non-vertebrates also follows a normal distribution but with larger standard deviation than that of vertebrates; Third, the insertion ratios of non-vertebrates are more negatively correlated than that of vertebrates.
Simulation testing
Default values for
As mentioned before, we start each simulation with a long exon. Using the counts for
For the remaining unknown parameters of GRFP,
These values are determined through an EM iteration process that will be discussed in the simulation testing section. The EM iteration uses observed values of
Relationship between α,
Using the values of
These simulations show that
Figure 6
Relationship between GRFP parameters and
Relationship between GRFP parameters and
From equation (9), we estimate that
Similarly, we performed a series of GRFP simulations with different choices of
In Figure
Figure 7
Plot of the number of splittings as a function of total CDS length
Plot of the number of splittings as a function of total CDS length. Estimated splitting (open circle) is from GRFP simulation with different CDS lengths (Table
Parameterizing GRFP via EM iteration
In the previous simulation studies, with the assumption of known
Before performing EM iteration, we need to quantify how
For EM iteration, we use
1. Given
2. With observed
3. With
4. With the relationship and
5. Return to step (1), iterate until convergence
The results of the EM iteration are shown in Additional file
Intron losses accounting for increasing
In Table
With simulated GRFP fragments, we gradually introduce 5-50% of "intron loss" by randomly reconnecting adjacent fragment pairs. The size distributions of the resulted fragments are fitted with a normal function, (Additional file
Additional file
Discussion and conclusion
In this study, we analyze the size distribution of exons for 14 species, including eight vertebrates and six non-vertebrates. Our approach overcomes the limits of using orthologous genes, thus allowing us to infer evolutionary processes affecting the exon-intron structure across widely divergent species. The use of size distributions is more reliable than alignment based approaches if considering the accumulation of repeating intron gain/loss. Based on the size distribution of exons, we propose GRFP to characterize the evolution of eukaryotic genes. The solid agreements between GRFP simulations and observations on genomic data provide several key findings on the evolution of exon-intron gene structures.
Chance of intron gain is proportional to exon size to the 3rd power
GRFP reveals that longer exons have a higher chance to gain an intron during evolution, and reveals the novel finding that the chance of intron gain is proportional to the exon length to the third power. This finding was derived after investigating real genome data, comparing with numerical simulations, and excluding various effects on GRFP through EM iterations. This finding might explain why long exons are rare in modern organisms. E.g., statistical study has shown that only 3.5% of the primate exons are longer than 300 nt
The "third power" is derived from
Why is the probability of intron gain proportional to the exon length to the third power? Given that the third power is usually related to volume, it might be possible that exon occupies a volume proportional to its length to the third power due to dynamic movement, and the chance of an intron attacking it is proportional to this volume. Further investigation will be needed to support this hypothesis.
No evidence for site-specific bias of intron insertion
We derive this finding from indirectly estimating the position distribution of intron insertion loci. We demonstrate that the insertion loci follow a normal distribution, peaking around the center of the exon with a standard deviation (
In Figure
The assumption behind the estimation of insertion ratio (equation (5)) is that the order of the exons within each gene is maintained during evolution. In the cases of tandem exon duplication, exon shuffling, or intron loss, the order is just locally disrupted. Simulation also shows that the estimated insertion ratio is a mixture of four different groups of adjacent fragment pairs (Additional file
Suggesting 5′ intron gain/loss bias
By grouping exons by positions within a gene, we demonstrate that exons next to the 5′ UTR have bigger standard deviation (
Excessive intron losses accounting for deviations from GRFP
In this study, we show that exons of non-vertebrates are different from those of vertebrates in three aspects. First, the size distribution of their exons fit a mixture of two normal distributions (Additional file
The estimations in Table
Here, the excessive intron loss hypothesis in non-vertebrate genomes is interpreted as breaking the equilibrium between intron gain and intron loss. Although GRFP model is built on modeling intron gain events, it does not assume that introns in vertebrate genomes are never lost. Instead, we interpret the straight line in Figure
The size distribution of exons (Additional file
Weakness and strength of GRFP
In this work, we propose the GRFP model to capture the dynamic processes describing the evolution of exon-intron structures. For vertebrate genomes, the model fits well with the well annotated genome data, including exon size distribution, distribution of insertion loci, total CDS length, number of introns, independency among intron gains, and 5′ intron gain bias. For non-vertebrate genomes, simulations show that the deviations from the vertebrate genome can be explained by excessive intron loss. The GRFP model implies that the evolution of gene structure is purely random, from picking which exon to split (gains intron) to picking intron insertion loci on the selected exon. The solid agreements between GRFP simulations and real genome data confirm that GRFP model provides one possible explanation on the exon-intron structure evolution.
It is well known that a modern genome is a collection of introns that have accreted (and been deleted) over at least a billion years. Here, by considering the whole process as a black box, we reproduce the output of this box (the current day genomes) with numerical simulations. The size distribution of exons serves as the key component in building GRFP model because of two reasons. First, the dominant factor that can shape such distribution is intron gain/loss (fragmentation). Second, the most prominent cofounding factor on exon sizes - the rate of indels in them during evolution is low. Certainly, the mechanism of intron gain is complicated considering differences across lineage, differences in rates of insertion across sites, the age of introns, the possibility of indels to maximize fit to epigenomic structures that can occur following intron gain, alternative splicing, the different models of intron gain, and so on. The process of exon fragmentation (or intron gain) might be as straightforward as the model of GRFP describes. By focusing on internal translated exons only, we have demonstrated outstanding agreements between empirical observations and GRFP simulations.
It is crucial to note that GRFP does not make any assumptions on the rate of intron gain/loss. Recent studies
One may argue that the agreement between the GRFP model and well annotated genome structure could be fortuitous. While we cannot rule out that other models might reproduce the exons of modern genomes, the predictive power of GRFP is striking, and we believe that it is a promising approach to understanding the evolution of exon-intron structures, and an excellent starting point for new models for revealing the hidden stochastic processes of evolution.
Unanswered questions and future studies
GRFP model provides explicit rules on the exon-intron structure evolution. However, it does not address the origin of introns, the mechanism of intron insertion, and the rate of intron gain/loss. In other words, GRFP addresses where introns are inserted (which exon and where in the exon), but not when and how introns are inserted. Future research will focus on extending GRFP to model the evolution of noncoding exon, and developing GRFP-based methods for comparative genomics studies.
GRFP: General Random Fragmentation Process; UTR: Untranslated region; CDS: Coding DNA Sequence; EM: Expectation Maximization.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
LW developed the model, performed the data analysis and designed the simulation experiment. LW and LDS wrote the manuscript. All authors read and approved the final manuscript.
We thank the National Science Foundation (DBI-0735191) and National Institute of Health (P41-HG02223) for funding aspects of this work.
The exon theory of genes
On the ancient nature of introns
An overview of the introns-first theory
Testing the exon theory of genes: the evidence from protein structure
Seven newly discovered intron positions in the triose-phosphate isomerase gene: evidence for the introns-late theory
Origin and evolution of spliceosomal introns
A detailed history of intron-rich eukaryotic ancestors inferred from a global survey of 100 complete genomes
Statistical features of human exons and their flanking regions
Exon size distribution and the origin of introns
Citation Classic - a Statistical Distribution Function of Wide Applicability
Spontaneous symmetry breaking in genome evolution
Concerning the logarithmic normal distribution principle of dimensions of particles during dispersal
Weibull Distribution of Particle Sizes Obtained by Uniform Random Fragmentation
Intron distribution in ancient paralogs supports random insertion and not random loss
Analysis of evolution of exon-intron structure of eukaryotic genes
Origin of introns--early or late
Mersenne twister: a 623-dimensionally equidistributed uniform pseudo-random number generator
An interior trust region approach for nonlinear minimization subject to bounds
On the Convergence of Interior-Reflective Newton Methods for Nonlinear Minimization Subject to Bounds
Ensembl 2012
Variation in the ratio of nucleotide substitution and indel rates across genomes in mammals and bacteria
Common exon duplication in animals and its role in alternative splicing
Relationship between "proto-splice sites" and intron phases: Evidence from dicodon analysis
Testing the "proto-splice sites" model of intron origin: Evidence from analysis of intron phase correlations
Exon recognition in vertebrate splicing
A phylogeny of caenorhabditis reveals frequent loss of introns during nematode evolution
Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss
Intron loss and gain in Drosophila
The biology of intron gain and loss
Divergence of duplicate genes in exon-intron structure
Identifying the mechanisms of intron gain: progress and trends
Whence genes in pieces: reconstruction of the exon-intron gene structures of the last eukaryotic common ancestor and other ancestral eukaryotes
The evolution of spliceosomal introns: patterns, puzzles and progress
Origins and evolution of spliceosomal introns
The origin of introns and their role in eukaryogenesis: a compromise solution to the introns-early versus introns-late debate
Did Genetic Drift Drive Increases in Genome Complexity


text:
After a while, I settled in the tiny flat above the Odd House Tavern. Things were relatively peaceful though I hadn't yet met anyone to mention. The boss was kind enough and for the first time it seemed I had found a genuinely quiet, normal life with a steady job.
Initially, it was nice. The regulars started to learn my name and it was almost like I had settled in. But deep down, there was a growing restlessness. Things were a little too quiet, a little too perfect. And the full moon was fast aproaching.

subset: commoncrawl


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(Update) Destructoid & Groupees team up for the hungry!
(Update: The kind folks at Groupees have revealed the first mystery game (pic below) and have given us five more bundles to give away!) Destructoid & Groupees have teamed up to raise meals for the hungry this holiday season. Every $1... read


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He tried hard, but failed.
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"The code that took America to the moon was just published to GitHub, and it's like a 1960s time capsule ," reports Quartz. Two lines of code include the comment "# TEMPORARY, I HOPE HOPE HOPE," and there's also a quote from Shakespeare's play Henry VI. In addition, the keyboard and display system program is named PINBALL_GAME_BUTTONS_AND_LIGHT, and "There's also code that appears to instruct an astronaut to 'crank the silly thing around.'"
A former NASA intern uploaded the thousands of lines of assembly code to GitHub, working from a 2003 transcription made from scans inherited by MIT from a Colorado airplane pilot, and developers are already using GitHub to submit funny issue tickets for the 40-year-old code -- for example, " Extension pack for picking up Matt Damon ". Another issue complains that "A customer has had a fairly serious problem with stirring the cryogenic tanks with a circuit fault present ." Because this issue succinctly describes the Apollo 13 mission in 1970, the issue has been marked "closed".


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This is the full research report for 22.214.171.124, which is an IP address.
Administered by APNIC
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Telephone: 91 4224377031
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wikiHow to Make a Lazy Mojito
When you don't feel like squeezing limes or making simple syrup, try this easy recipe for the lazy mojito that uses pre-made margarita mix.
Servings: 1
- 2 sprigs mint
- 4 oz. (120 ml) pre-made margarita mix
- 1 oz. (30 ml) light rum
- Ice
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featuring: Andrea, Ennui x Tongues, Alpman, Computer Emotions and Nyko
Tag Archives: Ennui
featuring: Galantis, The Kite String Tangle, Flight Facilities, MNEK and Diplo
being remixed by: Stonewall Klaxon, Kilter, Robag Wruhme, HVNS and ENNUI
ennui offers up a Telepathic Beat
featuring: TEEN, Local Natives, BANKS, Little Dragon, Lykke Li and Sailor & I
being remixed by: SAINT PEPSI, Jon Santana, Ennui, AIMES, Figgy and Âme
Ambient indie-pop trio Ennui dropped debut album Formation of Tides back in April. Stream it below – not sure how we missed this one but better later than never, right? Get heaps mellow and listen to their delicately crafted synth-wave tunes. Here's Ennui's debut in all of its glory [wp_bandcamp_player type="album" id="3167558477″ size="grande2″ bg_color="#FFFFFF" link_color="#000000″] Buy the LP via the […]


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The most important thing to pay attention to when purchasing a strap for your Luminox watch is that you are using an authorized Luminox retailer like US Navy SEAL Store. Straps purchased from unauthorized retailers might be fake and of a lesser quality than real Luminox straps. They might also not fit properly with a real Luminox watch and you will risk losing the actual watch if you use them.
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You must also be diligent in replacing your Luminox watch strap regularly. Typically, you should replace your Luminox watch strap every six to 18 months. How often you need to replace it will depend on the wear and tear that it takes and how well you take care of it. But even the most diligently cared for straps will still wear out and need replacing.
If you wait too long to replace your strap, it most likely will break and you will risk losing your watch or not being able to wear it until you purchase a new one.
So if your Luminox watch strap looks a little worse for wear, don't hesitate to order a new one from US Navy SEAL Store. We'll keep your watch on your wrist.


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*Spoilers* No Way Out Title Match & Sin Cara's Return Revealed
Published On 05/23/2012 | News
* It was revealed during Tuesday's WWE SmackDown taping in Wilkes Barre, Pennsylvania, that Alberto Del Rio will face Sheamus for the WWE World Championship at next month's WWE No Way Out PPV. Alberto Del Rio defeated Randy Orton and Kane in a Triple Threat Match to be named the number one contender for the Championship.
* Vignettes also aired during the SmackDown taping revealing Sin Cara will return to television next Friday, June 1.
* Click here for full WWE SmackDown spoiler taping results.
* Join the team and follow PWMania.com on Twitter at twitter.com/pwmania and on Facebook at facebook.com/pwmania.


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Staff Master
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Staff Master is an NPC class type.
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The following abilities have been verified on the Morgukai Chieftain, which is classed as a Staff Master. Weapon Damage for the Morgukai Chieftain was Kinetic Damage.


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Lace up boot made of patent leather in navy color. Bright, contrasting laces in beige. The upper holds and stabilizes ankle during walking. Flexible crepe rubber sole prevents sliding.
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ANGULUS was founded in 1904 by Willy Madsen's father, who had a cobblers shop on Nørrebrogade in Copenhagen. Willy was a teacher and disagreed with the pointed shoes that many children wore at that time. He thought they were both unhealthy for growing feet and hindered natural movement. In 1928, Willy astounded the Danish footwear industry with his new foot-formed shoes, developed in collaboration with doctors and physiotherapists.
ANGULUS today is a visionary brand. You can find a carefully selected but rapidly changing range of children's shoes.
Their perfect fit, quality and design ensured ANGULUS the prestigious British "Junior Magazine Deign Award" in 2012, 2013 and again in 2014, as well as the Danish "Børn i Byen" award for the best Danish children's shoe. Attention to craftsmanship and the guiding principle that children's toes should be able tomove freely in their shoes, drive the company onwards.


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hdnut Review
Easy to install replacing a 50's hanging light. Very quiet while on and easy to balance. The light attachment comes with two 40 watt bulbs, and after consulting with manufacturer we found that the new flourescent bulbs not only fit but give off even more light. Could not be happier!
- How did the color on site compare with the actual product? good
- Please tell us about the quality of the product. excellent
- How accurate was the on site description of the product? excellent
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|Chris Pepper <pep...@mail.reppep.com>
|Re: cvs commit: httpd-docs-1.3/htdocs/manual win_service.html
|Thu, 28 Dec 2000 23:39:49 GMT
At 4:52 PM -0600 2000/12/27, William A. Rowe, Jr. wrote: > > Index: win_service.html > >>
Effective with Apache release 1.3.15, the -k install option was added >> as an alias to -i, and the -k uninstall option was added as an alias >> - to -u. The original -i and -u options are depreciated in Apache 2.0. >> + to -u. The original -i and -u options are deprecated in Apache 2.0. >> These aliases were added to ease the transition for administrators >> running both versions.
 > >I'm not sure about depreciated vs. deprecated ... I've always thought in >terms of features being depreciated, but maybe that's just me. I'm not >trying to insult the old feature :-) I think of depreciated as equivalent to devalued. I'm using deprecated as in the HTML specs. If you feel strongly, or if someone else thinks I'm off base, I'll change it back. > >
Note that you may specify startup options on the apache -k >start command >> line, including the -D, -C and -c options. These affect the >>processing of >> - the service configuration, and may be used with the >><IfDefine> block >> + the service configuration, and may be used within an >><IfDefine> block >> to conditionally process directives. You may also override >>the server root >> path or configuration file with the -d or -f options. The >>options should >> also be passed to the -k restart command, but they are ignored if the >> service is running, and only processed if the service is started.
 > >You've changed the meaning here ... these are used with (or in >conjunction with) >the IfDefine block, but they don't literally appear there. Sorry -- reversed. Thanks, Chris -- Chris Pepper -- Resume: <http://www.reppep.com/~pepper/resume/> UNIX Meets the Mac OS: <http://db.tidbits.com/getbits.acgi?tbser=1186> Mac OS X Software site: <http://www.mosxsw.com/>
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i heard Emolga say "Emonga" in one part which makes me think they didn't change Emolga's voice from the japanese version.
Emolga and Pachirisu are pretty alike in some ways...
really liked this episode. emolga and axew are little buddies aww rlly cool
Excadrill being hit by Attract was just plain creepy.
I love Emolga, she's my favorite Iris pokemon. Axew is cute too, but I like Emolga better. My favorite part of this episode was definitely the part where Excadril gets affected by Emolga's attract. And I always enjoy having Bianca around.
New shinies week of 5/11-5/18: x2
New shinies week of 5/18-5/25:
Currently hunting/MM'ing: ; playing AlphaSapphire
F/riend codes: 4571-0519-1537, 4571-0519-6173
I still need some codes! Please add me and PM me, I'd appreciate it.
Emolga was a *****. XD But all my friends seem to love her for it.
Yeah Axew's innocence in that episode was adorable
Emolga's a pretty funny character. XD The dramatic 'acts' she make's...haha. XD
I'm a girl lol
PLEASE don't call me a 'guy'. Its a real pet peeve of mine with my autism and how my brain work's. thank you...
Gay, happy triox! Wait, gay also means happy, right? :P Riiight?
I have a MAJOR crush on korrina and i think she's totally awesome! best gym leader ever. Should be a champion.
I have autism and try to keep an open mind about things. My life can get weird.
I make interesting posts in the gay/lesbian/bi club and if you're curious about reading them feel free to check them out (and yes korrina is mentioned by me in there a lot :P haha)
My favorite pokemon is probably sylveon or mega lucario right now.
It seems like every third wild Pokemon the groups run into knows Attract, and someone always seems to catch the one they run into.
Ash - Snivy
Iris - Emolga
Bianca - Mincinno
There's something about Swoobat that makes it look disturbing and unsettling, If I'm honest. I think it's the eyes.
My top favorites
Claimed by me!
Formerly Painkiller2001
I'm not a fan of this episode and the one after it. It just seemed like the writers were trying way too hard to be funny with all of the weird gags... and I never liked Emolga nor did I see the point of Iris catching her, other than to show the genders of Ash and co.'s Pokemon, but we already have Snivy for that.
Iris' Emolga has to have had one of the most delightfully diffrent of the cast's pokemon, god I love it. Too bad it didnt end up doing all that much, but it was still a real joy to watch IMO. Watching BW after watching XYZ in general just feels pleasant.... Like coming home from a log boring walk, and taking a nap.
I totally expected Iris to catch Emolga this time since Dawn caught Pachirisu in DP. Delighted that Iris caught Emolga at least but didn't really care much about the other bits even though there were a lot of gags.
"The Dragon that is immune to Dragon and the Fairy that is weak to Fairy."
Sugimori art used; Peace to Dragons and Fairies
Banner created by me
Wow Emolga sure was sneaky. Her attempts to get food were funny and so was Oshawott's crush on her ha ha. I loved how she used Attract constantly and Iris catching her surprised me. ^^
Congrats to Iris for getting Emolga. Bianca definitely stole the show with this one, just going overboard in trying to catch Emolga lol. What is with Attract this season: is it the new go-to move for the animators? :x
I liked Emolga, though I wish that the dub had dropped some hints at how Bianca met up with the group. It feels unnatural to have character join the group off-screen with no explanation.


text: Listings Filter Sort by: Title Newest FirstRandom List Grid Map Rating & Up & Up & Up & Up Any Featured/Unfeatured Show featured only Claimed/Unclaimed Show claimed only VEROPOULOS DOO Supermarket chain - retail Milutina Milankovića 86a, Beograd +381 11 3119 111+381 11 3119 111 +381 11 311 5501 SUPER VERO is a famous supermarket chain and member of VEROPOULOS SUPER MARKET GROUP which has a total of 233 stores. Throughout the whole of Greece it has 220 stores and outside of Greece, supermarket chain is present in Serbia and FYROM. In 2002, the Group opened the first 6.000 s.m. super market under the logo Super Vero in Belgrade. It became the first foreign company that bought land in Serbia. Redo search when map moved Showing 1 result


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Room 15Mature
Me and Rai link arms and go to our class. Our outfits are almost identical, except my skirt is blue as well as black and she has loads of bangles. We reach a door with a little metal sign that has 15 engraved on it. Rai knocks and we go in. The teacher looks at us and says "Oh hello, you must be Rai and Ebony Night, you can have the seats at the back, over there." I follow Rai and sit down.
"Everyone keeps looking at us," Rai whispers in my ear.
"Rai, we're both tall, slim compared to most of them except those practically anorexic chavs over there, complete opposites even though to them we have the same last name. We're new, and we joined halfway through the term. No one knows who the hell we are," I whisper back, whilst watching the teacher explaining something. Then suddenly a shrill siren wails, I recognise it as a fire alarm. Everyone gets up and leaves, me and Rai follow then to the car park. I see Sparkle and Ryan and run over to them.


text:
I walked down the hall way and up a flight of stairs. There was no need to take the elevator when I was only traveling up one story, it would be more exercise in my day. I looked at the names taped to each of the doors until I found Lenna in bright blue and orange font decorated with a matching butterfly over the final a.
I knew before she opened the door that her small room would be blue instead of pink like mine. Every other floor coordinated between the two colors like a never ending baby shower of ambigous gender. Lenna answered the door with a tired smile, "oh, hi Joan. What brings you here?" "Hi, sorry to drop by with out any notice. I was just wondering if your roommate had arrived yet."


text:
Online 8 months ago
Rent budget
Preferred stay length
Move date
I'm a chill guy, mostly keeping to myself but definitely enjoy to hang out and have a couple of beers every now and then. I'm an avid hiker and camper.
I moved to Australia from South Africa about 8 years ago.
I'm working full time as a software developer and I'm studying part time.
I always keep my space and common areas clean.
I'm happy with short term stays but prefer 3+ months.
Hans-Albert is also interested in Teaming-Up and starting a new share house.


text:
|Reviews for Something we need to talk about
| Miss Snazzy chapter 1 . 5/18/2013
Good one-shot. And what a great way for Damon to approach the vampire-topic. Lol.
| AngelofTheDawn08 chapter 1 . 5/8/2013
sequeal! Please? :(
| seaoftrouble chapter 1 . 4/27/2012
That was niiiiice. I really loved the last line!
| WiseDraco chapter 1 . 3/3/2012
I like to think I'm a decently talented writer of sorts but how in the bloody fu(k did you make a serious conversation over a blowjob so hot and touching? Damn you and your obviousness talent.
| SweetWillowTree chapter 1 . 2/22/2012
Most people, on waking up naked and tied to a bed, with a vampire standing idly by and waiting for them to open their eyes, would do something dramatic; scream, perhaps, start begging for their lives, tug at the restraints.
Alaric Saltzman figures it must be a Thursday.
- I don't even need to say how funny this is.
"I dunno, man. You tied me up and lit candles. That says 'plot' to me."
- And this.
"Because you're far less chatty post-orgasm, and I haven't finished talking about this."
- I imagine him stomping his foot.
| FlyingPigMonkey chapter 1 . 2/11/2012
now that's just plain mean! but oh so awesome...
| Princess Of Souls chapter 1 . 1/28/2012
;) epic! :)
| Tunz N Cruzn chapter 1 . 1/6/2012
| SpaceDementia49 chapter 1 . 1/6/2012
| CrowX chapter 1 . 1/3/2012
Hahaha, evil Damon. Really, torturing Rick like that.
But I oh so wish that he gets his positive answer. I mean, after all that 'work'.
Looking forward to a second chapter/sequel, where Alaric agrees and doesn't regret it.
| Mineola chapter 1 . 1/2/2012
I am so grateful this ship has you to fill up the lack of dalaric fics :) you're the best and this story was great lol and so typical
| DoodeSeriously chapter 1 . 1/2/2012
jesus, damon is one evil bastard. hahah
love it! and hate it cause poor ric is suffering!
please please please continue... pleaaaase!
| kat4543 chapter 1 . 1/1/2012
i just got to know is there more?
| starzee chapter 1 . 12/31/2011
This is seriously hot. The beginning had me in fits of laughter.
So much of it is totally quotable so if I start that I will no doubt just be recounting your story so I won't lol. Can't wait for more though it says complete which can't be true because I need to know what happens! lol
Happy New Year!


text:
Recanto Cataratas Thermas Resort & Convention
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- Tennis Court
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Photos of Recanto Cataratas Thermas Resort & Convention
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- Tennis Court
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TripAdvisor Reviews Recanto Cataratas Thermas Resort & Convention Foz de Iguacu
Travel Blogs from Foz de Iguacu
Iguassu falls - what a day
... I can't try to describe the magnitude of the falls just to put it on to perspective it took us 4 hours to walk around the Brazil side of the falls and 500 pictures later plus more rain and spray that you can handle we finally got a taxi back to the hotel. This is a must do before you die and I'll post some pictures when I have better wifi and a top tip is to purchase ...
Back to Argentina!
... a bar in the other. Me, Harrison, Paul and stu spent most of our night here. Sophie and Neill went back to the hostel fairly early. An Irish girl and her friend who we met back at re hostel were also with us. It was almost like I had passed my drunken state onto the Irish girl like the flu because she could not stand! Being the gent he is, stu stepped in to assist her into a taxi and said he'd be back shortly. Paul had buzzed off somewhere too so it was just ...
275 waterfalls and meat sweats in Foz do Iguaçu!
... time to say "Tchau" to Brazil... But we are going to go out with a BANG. Well, more like a roar... A roar of 275 waterfalls to be more exact. OH YES. The ridiculously amazing and mind-blowing Iguazu Falls are our last stop in Brazil and also first stop in Argentina, as the falls fall in both countries and you can visit them from both sides... Truly incredible. This has been, for me personally, the most breathtaking part of our travels here in ...
Crossed one off the list
Well !!
We arrived in Foz do Iguazu after a Looooong bus journey from Sao Paulo. We saw a guy we met in Paraty hostel on the bus. We arrived in Foz do Iguazu at 10.30am but couldn't check in until 2pm so we went for some brunch then came back and hopped on the bus to see the water Falls.
There were tours going from our hostel but the public bus was a fraction of the price so we did it ourselves !
The views were breath-taking and the photos don't do ...
One of the Seven Wonders of the World.
... We have been blessed to travel a bit, and sometimes I worry that we will become jaded to the sense of wonder traveling can bring.
There are so many times that we will be doing just every day living here and suddenly realize that we are living in Paraguay. It is a feeling of "Shock and Awe."
We loaded up and headed towards Brazil. A six-hour drive. We arrived in Ciudad del Este, the ...


text:
Is there a way of somehow incorporating asset management and barcodes with the Spiceworks application? We are looking into using barcodes for our inventory and wanted to see if Spiceworks could possible work.
2 Replies
Oct 26, 2011 at 12:59 UTC
I would love to see physical asset management tied in to the network based inventory module. Good idea.
Oct 26, 2011 at 1:15 UTC
Pittsburgh Computer Solutions is an IT service provider.
You can add devices manually and track all the information. As far as barcodes, alot of the handheld systems just scan the bar codes and as long as it is connected to your computer you can scan the barcode directly into the tag field.


text:
Kaufman's 'Anomalisa' in Venice is animation: for adults
By Michael Roddy
VENICE (Reuters) - Director Charlie Kaufman's "Anomalisa", shown at the Venice Film Festival on Tuesday, is a stop-motion animation film shot with puppets that is full of laughs, tenderness and more than a touch of the surreal.
But with an explicit sex scene between two of the puppets about halfway through, it won't be one for the whole family.
Kaufman refuses to discuss what his films or screenplays are about, but he and co-director and animation specialist Duke Johnson told Reuters in an interview they can live with the U.S. R-rating the film got because of the sex scene. It means viewers under 17 years old must be accompanied by an adult.
"We're fine with that ... I mean we wanted to do it in a way that was emotional because immediately people think puppets having sex is a joke," Kaufman said in Venice, where the film is competing for the top Lion d'Or prize.
"You know 'Team America' did it and it was done for a joke and ... it doesn't fit with our story to be that. It's an emotional moment ... so we worked really hard to make it that, to make it real and sensitive."
Kaufman, who wrote the screenplays for "Being John Malkovich" and "The Eternal Sunshine of the Spotless Mind", has not been a presence in the cinema since he made his directorial debut with the highly regarded but quirky "Synecdoche, New York" (2008).
He is making his return with this 90-minute film focusing on a motivational speaker named Michael, who flies into Cincinnati from Britain on a soul-sapping business trip.
Suffering from the type of identity crisis for which Kaufman characters are famous, Michael meets up during his one-night visit with an old flame, who has never gotten over him dumping her, and a woman named Lisa, who has come to hear him speak and who gives Michael new inspiration for life. Continued...


text:
Open Forum: Time for petty discrimination to end
It's estimated that a GLBTQ youth is three to seven times more likely to commit suicide. A large reason for this is the harsh bullying that occurs far too often, especially in schools. The worst of the fact is that most social, political, and educational institutions do little to stop this prejudice. They rely on you to stand up and point out injustice. Speaking from my own personal experience, when I was going through school kids apparently knew I was gay before I even did. I was harassed for a large part of my education by students who would use "fag" and "gay" in derogatory ways, as well as threaten me. I was still one of the lucky ones who was never physically assaulted. Sometimes these awful events would happen in front of teachers, adults, or peers who would do nothing about it. Eventually I took action.
Coming out of the closet my junior year, I took a leadership position in the school's Gay-Straight Alliance and began my continuing efforts to promote justice and equality, and to provide a safe space to my fellow members of the GLBTQA community. It's time we reach an understanding and that this petty discrimination ends. Gay, lesbian, bisexual, and transgender students deserve the equal rights and respect that everyone else should have. It's time to make a stand because it won't simply get better unless we make it better, and there is always power in the individual voice. Stand up, be a part of the solution, there is always work to be done.
Nicholas Swenson


text:
Discussion in 'Random Thoughts' started by RussellJ, Nov 6, 2011.
You guys are doing well!
Sent from my HTC EVO 4G using Tapatalk.
Where just trying for the first pick in the draft.
Well looks as though they are overachieving at underachieving
hiding with the bama fans?
I guess this season justifies Manning's salary.
It goes to show how good manning is and how bad the rest of the team is. Having a good offence keeps your defence off the feild so it can rest. I hope he stays out the enitire season so he can heal, if he comes back all he will be doing is messing up there draft and rosking his career.
Charlie, I agree just call this year a waste. He needs to be 100% for next year. I think the strike really made it even worse.
1pick need a replacement for manning
I am not sure if he even plays much over another year
One wrong hit and he will never walk again from what they are saying
old school Steelers fan here (if I even watch it anymore)was cool they beat the Colts at home..the game my wife and son went too
Trust me when I say...don't say that around Indy (bama)


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text:
Lucknow, May 2 (ANI): Uttar Pradesh Chief Minister and Bahujan Samaj Party (BSP) supremo Mayawati has reportedly expelled about 5,00 party workers and leaders for dubious and criminal intentions with immediate effect from the party.
Those expelled include Mirzapur Division Zonal Coordinator Narain Das Ahirwar. He has been expelled for not implementing the directives of the party supremo.
It has been reported that the decision to expel the party workers was taken after Mayawati's discussion with the zonal coordinators at Lucknow.
During the two-hour-long meeting, excluding Mirzapur zone, reports submitted by all other coordinators were found to be correct, and on the basis of that they were expelled from the party.
Meanwhile, the Bahujan Samaj Party has alleged that Samajwadi Party and other opposition parties conspired to get entry of leaders and workers with criminal and dubious back ground to the ruling party.
Lashing out at the Opposition, Mayawati on Sunday said they have done this with the objective of keeping anti social elements and criminals belonging to their fold, out of jail, adding that such elements have been identified and BSP has shown the door to all of them.
She said that senior BSP leaders have been asked to recheck about criminal elements of those remaining in the party and submit their report by May 13.
Further criticising the Opposition, Mayawati said they have nothing substantial against her government, so they are making perverse statements to defame her government in the name of data of crime activities. (ANI)


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text: Thomas Pesquet is a European Space Agency astronaut of French nationality. He is currently on a six-month mission to the International Space Station. Thomas is serving as a flight engineer for Expeditions 50 and 51, launched in November 2016 and returning in May 2017. He previously worked as an aerospace engineer, and is also an airline pilot for Air France.


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Incredible Story: Nate Winters is Pitching Again
High School
: : General
Jim Ecker
Friday, April 02, 2010
Nate Winters loves baseball and dreamed about playing in the major leagues. He played for successful travel teams when he was younger, the type of teams that routinely produce Division I players and top prospects, and he was coached by former big leaguers along the way. His father owns a minor league team in Florida, so he grew up in a baseball environment, soaking it all in. His future looked bright, a baseball career on the rise.
Then came Aug. 5, 2008, the day Nate lost his left leg in a terrible boating accident and nearly died. But in a remarkable story of spirit and determination, he's pitching for his high school team again.
"Pretty amazing," said Bob King, his coach at Winter Park High School in Florida. "To me, it's miraculous."
Nate was 15, enjoying his summer vacation when the accident occurred. He was boating with his older brother, Zach, and three others on a hot afternoon near their home in Winter Park. Zach, who was driving, saw grass ahead in the lake, so he steered the motor boat sharply to the left, going about 25 to 30 mph. It wasn't a reckless move, but Nate got tossed into the lake and went under the water.
The power boat went over him. He got hit by the propeller, nearly cutting off his leg. It later had to be amputated at mid-thigh.
"My whole body was numb," Nate told Perfect Game. "I looked down and my leg was all cut up."
Somehow, he kept his senses.
"Nate saw a bunch of blood in the water," said his father, Dr. Tom Winters. "He was afraid an alligator was going to come get him, so he started swimming himself back to the boat, and that's what saved his life."
Zach jumped into the water and helped pull him out. The people on the boat quickly applied a tourniquet to Nate's leg and called 911. Nate doesn't remember too many details.
"I kind of blacked out," he said. "I was in some pain, but I think I went into shock."
An emergency helicopter arrived at the scene within minutes and landed on a nearby soccer field. Nate was rushed to a trauma center, where an expert medical team went to work. They couldn't save the leg, however. It was too far gone.
"It was hanging on by strands," his father said.
The leg was gone, but Nate was lucky to be alive.
"Statistically, the odds of getting out of the water alive are about 1 in 100," said Tom Winters, a medical doctor. "And when your hemoglobin goes down to where Nate's did, your chance of surviving is about 1 in 10,000."
Nate lost 80 percent of his blood, which is normally fatal.
"He's a real strong kid," Tom Winters said.
Dr. Winters rushed to the scene of the accident, not knowing what to expect. He's witnessed a lot during his medical career, but this was his son. It was obviously a grave situation.
"I said, 'Nate, are you OK?' And he kind of grabbed my hand and said, 'Dad, I lost my leg,'" Dr. Winters related. "And then he said, 'Am I going to live?' I said, 'Nate, if God got you this far, you're going to live.'"
Emergency medical workers at the scene put "trauma trousers" on Nate, in order to apply pressure and stem the bleeding, but Nate didn't know what was going. In fact, he feared the worst.
"Nate thought they were putting him in a body bag," Dr. Winters remarked. "Nate said, 'Don't put that on me. I'm not dead yet.'"
Nate also lost a toe on his right foot in the accident, and the Achilles' tendon on his right leg was severely cut in several places. Family and friends gathered at the hospital, more than 100 strong, frightened, praying, distraught. It was a long, tough night, but Nate fought through it. He woke up the next morning.
"The first thing Nate did when they took the tube out (of his mouth and throat) at about 7 o'clock the next morning, he turned to Zach – my oldest son – and said, 'Hey, thanks for saving my life,'" Dr. Winters said. "So Nate kind of made him feel good."
The accident happened 20 months ago. Incredibly, Nate has bounced back, with a prosthetic left leg, great spirit and tremendous determination.
Remarkably, he pitched two scoreless innings for the Winter Park High School junior varsity team last week. His fastball was probably in the high 70s and might have touched 80. And having passed that test, his next assignment could be for the varsity.
He's back.
"I have to admit, I had my doubts," said Coach King.
Nate, now 17 and a junior, is playing again. And having fun. Even Nate is surprised.
"I was always doubting myself, if I was ever going to play again or not," he said. "It felt pretty good."
The junior varsity game was played at Dr. Phillips High School and drew a big crowd, unusually big for a jayvee game. They were there to see Nate, to cheer for Nate.
"When Nate got out there to pitch, the other team gave him a standing ovation," Tom Winters said. "And after he finished, their team's fans gave him a standing ovation again. There were a lot of goose-bumps going around the ballpark that day."
It was a long, hard road back.
Nate spent 10 to 12 days in the hospital after the accident, then his injured Achilles' tendon got infected. He had to go back for more surgeries and more treatments. He ended up having nine operations and spent several months in a wheelchair, but he gradually got stronger.
Tiger Woods, who lives nearby in Florida and heard about the accident, sent Nate an autographed picture and a card. It said, "Nate, be tough always."
Milwaukee Brewers slugger Ryan Braun, who played for Tom Winters' minor league team – the Brevard Manatees – sent Nate a baseball and bat. The Boston Red Sox sent a Jonathan Papelbon jersey. Jason Varitek's parents showed up. So did Zack Greinke's father. "The major leaguers have been great," Tom Winters said.
This past fall – about 14 months after the accident – Nate played catch with a friend and tried some bullpen sessions. That's when he began thinking about a comeback. He decided to go out for the team, but it was rough. He lost his balance and fell off the mound a few times until he got accustomed to landing on his prosthetic left leg.
"He got pretty disenchanted and a little embarrassed," his father said. "He called me about 6 or 7 weeks ago and said, 'Dad, I can't do it.'"
As it turned out, he could.
"I love playing baseball," Nate said. "I kept going."
The prosthetic device allows some flexibility, and they're making adjustments on the fly. "There are some things that are not perfect with it, but it's pretty good," he said.
Nate's life has changed in many ways the last 20 months. He's developed into a talented musician and performs in a band, playing the piano and guitar. His grades have improved, he was elected junior class president, and he's playing ball again.
The next step will be pitching in a varsity game this year. It will happen, because Nate deserves it. He's earned it.
"Oh, yeah. He's going to get in a game. It's just a matter of when," said King. "And seeing what he's done, I think next year he'll be a very important part of the team."
It's amazing. And the story isn't done yet.
"Who knows?," Tom Winters said. "If he goes to the right college, maybe he'll be a relief pitcher for somebody."
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The Snow
Book by: Cody S. D. Crum
Status: Finished
Genre: Thrillers
Chapter10 (v.1) - Ol' Mum
"Oh good she can come," Kelsey said as she set a lid over some sizzling pot stickers on a frying pan. Dwayne was sitting on the kitchen counter across from her and nodded when she looked over her shoulder, "You gonna meet her there?"
"I'm picking her up."
"I can give you guys a ride," she offered.
"No, I'm taking her home too. I'm not going to make you drive half an hour to Thorp and back." Thorp probably wasn't really a half an hour away. It was only a ten mile drive down the interstate, and her house was just another two miles off of the highway. But his mother had never been there and probably didn't know that for sure.
Kelsey looked at him with a discerning eye, "You need to clean the inside of your car then." She turned around and lifted the lid and rotated the pot stickers. "Especially if you're hoping she's gonna kiss you when you drop her off." She looked over her shoulder again with that same shrewd eye, but a grin at the same time.
Dwayne grinned at her but kept his mouth shut. Not that it mattered all too much. If he admitted to his mom that, yes, he did plan on kissing Allie in his car, she probably wouldn't protest. Kelsey like all mothers wanted her son to be happy and happened to also fit into the minority of mothers who believed kissing was clean and healthy. She explained that a certain amount of affection was always nice to have. As long as it never went too far, at which point she didn't want to see or hear about it.
They both stayed in the kitchen a while not talking. Kelsey stayed focused the food, poking it now and then while her son sat quietly enjoying a calm evening. Dog was sitting by the back door and looked at Dwayne giving him a long sad cry, his tail flicking back and forth.
"His leg was pretty banged up" she said looking down at the cat. "Remember that he's not allowed outside at night anymore." She looked at him for affirmation.
"I know ol' mum."
Kelsey looked at him actually scowling slightly, "You may call me many things, but do not call your mother 'ol' mum'." She said pointing a threatening spatula at him. "Now go wash up."
© Copyright 2017 Cody S. D. Crum. All rights reserved.
Add Your Comments:
Haha. The end, I liked it very much. Nice little chapter. Have a greatest day everyday!
Kelsey is so complex. She seems more in touch with her youthful self than most parents, adding to the irony of Dwayne calling her "ol' mum."
While I hate to over complicate, the lives and interactions of the mother and son are supposed to be as complex and real, as they are as simple to a family who has experienced such a relationship. I hope you find them to be real, whole, well rounded people. Not just names in a story. It is for stuff like this I much recommend all writers create character drawings. To help them remember and mold who it is they are writing about.
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Founded in : 1887 A.D.
Founder : Shiv Narain Agnihotri
The Deva Samaj Movement was founded in 1887 A.D. in Lahore (now in Pakistan) by Shiv Narain Agnihotri. Shiv Narain was also influenced bv* the teachings of the Brahmo Samaj. Deva Shastra is the book of teachings of the Deva Samaj. Like other socio-religious reform movements the Deva Samaj also worked to eradicate many evils prevalent in that time society like gambling, intoxications, violence etc.
Principles and Objectives
Major teachings and objectives of the Deva Samaj are as follows:
- Deva Samaj believes in eternity of soul.
9 It believes in the existence of a 'Supreme Power'.
- It also recognizes the supremacy of the Guru.
- It emphasizes a human life based on good deeds and principles.
- The Deva Samaj prescribed ideal social conduct and moral ethics, such as not accepting bribe, abstaining from gambling, intoxications, violence etc.


text:
Author note: I do not own Count Cain: Godchild. Nor do I own Cain or Cassandra.
I swear, I bet some of you guys are thinking something along the lines of 'Why...in the world...are Cain and Cassandra of all people the main people of this fic?' or 'What the heck! How in the world could these two ever cooperate!?' Calm down. This is an AU fic. Anything can happen. Just thought I would remind you readers out there.
Also...this fic is dedicated to storm101 because this author writes awesome fics, such as 'Quite Contrary.' I don't know if storm101 is into the genres of drama/horror/suspense/supernatural, but...I will do my best, at the very least, to make sure everyone is in character. Or at least I hope I can do so.
Warning: AU fic, takes place pretty much after the Castrato arc (so that means there may be spoilers), mentioned abuse, violence...probably no pairings as of now.
Constructive Criticism would be great, and I hope you guys enjoy reading the fic! Thanks!
The sound of water droplets hitting the concrete floor was what roused Earl Cain Hargreaves from his unconsciousness. The earl sat up quickly staring around. The room was dimly lit, candles being the only light source, and water dripped from the ceiling onto the dusty, dusty ground. He could only see a door, and he had a good feeling it might be locked...
"So...you finally woke up. How long was it since you were thrown in here, Earl Hargreaves? One, two hours maybe?"
Cain immediately recognized the voice, deep and like a blood-orange, its sweet innards dripping with a sweet sort of venom. However, the voice sounded much more...tired, exhausted. He turned, recognizing the silhouette in the shadows, sitting in a corner of the room. Cain spoke in response.
"Lord Cassandra Gladstone, isn't it?" Cain demanded. The sihlouette nodded its head silently. "What is this place?"
Cassandra seemed to hesitate before speaking. "We're...locked up, Earl Hargreaves. I think that part is quite obvious. The Cardmaster ordered it...when you...humiliated me at the operahouse...I was captured a little later by your half-brother...and that...that...Trump Card..." he said the words 'Trump Card' with an air of disgust. Cain had a feeling Cassandra took a strong dislike to Jizabel's assistant, Cassian.
"So...you are an agent of DELILAH after all." Cain paused before speaking again. "And what part of DELILAH were you associated with, exactly? Another of the Major Arcana?"
Cassandra let out a cold laugh. "Ha! That's completely correct. I was no. 5 of the Major Arcana, the Hierophant...I initiated Jizabel into the Major Arcana...he took the position of Death. When I was captured, I was stripped of my rank, and...well...it led to this."
It was only then that Cain wondered why Gladstone wasn't making any attempt to approach him. He thought if he were Gladstone, he would have walked over to Cain and tried to strangle him, as revenge for humiliating him. But Gladstone wasn't moving from his spot at all.
"Let me see your face."
"Do you think I take orders from you!?" Cassandra rasped, obviously offended. But then his tone of voice softened. "Do you really want to see me? And are you sure about that?"
"Yes and yes." Cain responded, crossing his arms and trying to seem cold, intimidating. There was silence before the older man responded.
"Fine. I'm sure you wouldn't regret it. But you better not laugh...or I may attempt to strangle you." Cain had a feeling that the threat was empty. Gladstone seemed much too weak to do so; he even appeared to have a hard time getting up at first. He finally stood, using one arm against the wall for support, and slowly, cautiously, walked forwards slightly.
Cain couldn't help but stare in horror at the ex-Hierophant.
The candlelight illuminated the pale, cream-coloured wing that seemed to stick out from Cassandra's back, clots of blood from the base of the wing. There were scars all over Cassandra's chest, scratches on his face and arms, and his hands were clawed. There were burns-most likely due to contact with acid-covering the abdomen and the left side of his face, making him look very grotesque, and reminding Cain of the time when all those ladies had their eyes gouged out due to the parasites within them...
"They experimented on me...and they used the whip on me...just like they did to you and Jizabel." Cassandra's voice seemed to quiver slightly. "And now they've left the both of us down here to die."


text:
Last year, student campaign group People & Planet published its controversial Green League purporting to show the environmental performance of Britain's universities. As reported in The Independent, many institutions – particularly those in the must-try-harder category – dismissed the table's methodology as "sloppy" and "dire".
One thing is clear though: everyone is keen to be green and be seen to be green. Durham University, which scored a 2:2 in the People & Planet League (which the university says drew on out-of-date information), is taking the matter seriously, recruiting Antje Danielson as deputy director of sustainability.
Danielson oversaw Harvard's Green Campus Initiative, which saved the American institution £100,000 in the first year. "The numbers at Harvard are staggering now, with the initiative annually saving $7m – the cost is $2m so that's a net benefit of $5m," says Danielson.
Durham is the only university of the 20 supplied by N-Power to use 100 per cent green energy, saving 32,000 tons of carbon emissions over the life of the 24-month contract. Its procurement service focuses on fair trade and sustainable practices, and uses the university's buying power to influence supplier behaviour.
The students are also active, running an Allotment Society to grow their own produce and introducing a "Green Move Out Scheme", encouraging the recycling of clothes at the end of term. The difference has been noticed by students. "When I arrived I didn't think they [the university administrators] were that responsive to these kinds of issues, but now I can see they are really trying and some of the changes have been amazing," says Anthony Crowther, 20, a second-year geographer and the Student Union's environment officer.
Bureaucratic inertia, EU law and a legacy of old, energy-hungry buildings can make it difficult to effect change. New-build programmes offer a chance to green the campus: new-build work at Greenwich University's Avery Hill campus, for example, will include timber from sustainable sources, solar water heating and a green roof – topped with perennial plants – offering a natural habitat for birds and other wildlife.
De Montfort University is planning to introduce biomass boilers – which run on renewable fuels – in its new buildings, and is using new types of glass and solar control film to make its buildings more energy-efficient. This September, Newcastle Business School at Northumbria University will move to a new £70m state-of-the-art green building. The new-look business school has been built using 95 per cent recycled materials from an old cinema. It has a floor-to-ceiling central light well that allows natural light to penetrate the middle of the building (reducing the need for artificial light, which will be supplied via an intelligent lighting system that shuts down when not required), and rainwater is collected in underground tanks to flush toilets.
Green travel plans are also much in favour across the HE sector. Northumbria University, for example, is implementing the provision of 160 secure bike places, with showers on site for perspiring pedallers. Manchester Metropolitan University (MMU) has had a green travel plan in place for a few years, working alongside its Oxford Road Corridor partners – the University of Manchester and the Royal Northern College of Music – on initiatives such as car sharing, bus-route planning and cycle facilities. Now MMU is planning to go further, as it invests £250m on its estate over the next five years and consolidates from seven to three campuses.
This consolidation will impact surrounding communities and, says director of services Mary Heaney, the university is keen to make sure that impact is as positive and green as possible. The university is mapping the location of all its staff and providing them with travel alternatives, incentivising the greenest options; those who car share, for example, will be more likely to be allocated a parking space.
"We're also looking at flexible working arrangements to avoid congestion and we're going to introduce video-conferencing so that we can reduce intra-site travel," says Heaney.
Universities have one advantage over other organisations: they can draw on in-house research and expertise to optimise their green policies. London South Bank University's (LSBU) Borough Road building can boast Sunmount, a solar-panel energy system aerodynamically designed to withstand winds up to 112mph and developed by an LSBU spin-out company called Solion.
Academics also help shape government policy and develop green technologies, reducing the need to convince university staff. Our universities may be a long way from being carbon neutral, but they're going about it the right way.
- More about:
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text:
New emissions standard – what does this mean for Wheelchair Accessible Vehicles (WAVs)?
In recent years, legislation has been introduced to reduce the level of air pollution emitted from road transport. This means that car manufacturers have had to start developing newer and cleaner engine technologies in order to meet the need for improved air quality.
A new emissions standard introduced in September 2015 (Euro 6 Emissions Standard) requires manufacturers to make cars cleaner by reducing the levels of harmful exhaust fumes, in particular nitrogen oxide.
As WAVs are converted for comfortable and safe wheelchair access after the initial vehicle is built, converters must follow a slightly more complicated process in order to ensure that vehicles registered from 1 September 2015 meet the new emissions standard. In the short term, this means it could take a little longer for a new WAV to arrive, as converters engineer solutions to meet the new regulations.
Not all WAVs are affected in the same way, so it's worth discussing delivery times of the vehicles you are interested in with your chosen converter. If you do find there are some delays to your preferred vehicle, you may wish to consider a WAV that the converter already has in stock as it may be available to you more quickly. You could also consider a Nearly New WAV as they are also often available in a shorter time period. However with both these approaches, you may have to compromise on either your preferred colour choice or the vehicle specification.
Whichever option you select, it is important to choose a WAV that fully meets your needs both now and for the future, even if this means it takes a bit longer to arrive.
If you already lease a WAV on the Motability Scheme and you encounter delays when ordering your next vehicle, rest assured we will be able to extend the lease on your current vehicle to ensure that you stay mobile during this time.


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Playstation Now Game Requests[ Edited ]
I've been beta testing Playstation Now since PS4 released it to the community for testing. I've noticed within the last month, there have been bigger titles released, but also there are games I never got to try on PS3 or would like replay value. I request the following titles;
* Heavy Rain
* God Of War (Saga)
* Infamous
* Infamous 2
* Uncharted: Drake's Fortune
* Uncharted 2: Among Thieves
* Uncharted 3: Drake's Deception
* Killzone Trilogy
* Little Big Planet 1 & 2
* Resistance Trilogy
* Twisted Metal
I find that I'd only buy into this service if there were actual Sony exclusives mixed in, otherwise it just fails to make me want to continue to use Playstaion Now.
Edit: Edited title for clarity. - space_paranoids
Re: Playstation Now Request Games
MRizkBV wrote:
Saints Row: The Third (PS3)
Saints Row IV (PS3)
Digimon Rumble Arena 2 (PS2)
Assassins Creed III (PS3)
A million times yes.
Also, most of the games that have been mentioned are currently almost dirt cheap for ps3, or were once freebies on PS+. You can get all three uncharted games for like $20, resistance trilogy for $20, and the killzone trilogy for $20 (i've heard 1 sucks, but I've only played 3)
Re: Playstation Now Request Games
I would like to see, remastered collection. Running 1080p 60fps.
- Uncharted: Drake's Fortune
- Uncharted 2: Among Thieves
- Uncharted 3: Drake's Deception
Re: Playstation Now Request Games
Re: Playstation Now Request Games
"Winner: SOCOM II: U.S. Navy Seals (24.74%)"
It's a great day to be a SOCOM fan!!!!!!!!!!
Awesome job SOCOM fans, Worldwide!
Re: Playstation Now Request Games
Re: Playstation Now Request Games
Also, all those SOCOM games are Sony exclusives, meaning Sony own all the rights and and code related to those games. If Sony wanted they could simply copy and paste those games onto the psnow servers. Can you post a link where Sony says that old "shut down" games won't be on psnow?
Nobody know what games will be on psnow when it's ready for public release, until then I will continue to list games I wish to see on psnow, especially in threads that ask for our psnow requests.
Btw, you've posted twice in this thread but failed to mention what games you want to see on psnow. Are you trolling or just trying to get your post count up?
"Winner: SOCOM II: U.S. Navy Seals (24.74%)"
It's a great day to be a SOCOM fan!!!!!!!!!!
Awesome job SOCOM fans, Worldwide!


text:
Cars Again
It was then that Luke happened upon a derelict car. At first he was going use it as said obstacle to hide behind, but another idea hit him. He could, of course, drive away (for his life) in the car.
Luke jumped into the red Ford, slamming the rusting door unsteadily in a certainly conspicuous way. He turned the ignition unconfidently and slammed his foot down. Déjà vu popped into Luke's mind as he ran away.


text:
Breaking Dawn will be the movie of the year, and the part we're obviously most excited for is the romantic wedding between Robert Pattinson & Kristen Stewart! Although everyone involved has been quiet about what the ceremony will look like, we've gathered every bit of info we can about the big day to get an idea of how beautiful it will truly be. Check out our gallery of the BEST coverage of the Breaking Dawn nuptials!
'Flightless Bird, American Mouth' is the couple's wedding song!
Kristen said it was the most emotional role of her career so far!
Jackson Rathbone said both Kristen & the wedding scene are 'stunningly beautiful!'
Shooting the scene was a long and difficult process!
Robert called the ceremony 'momentous!'
Kellan Lutz says that the wedding will make fans 'swoon!'
Rob called the ceremony 'strange' & 'embarrassing!'
Click here for more 'Breaking Dawn' premiere pics!
Thanks to her enviable frame, penchant for chic minis, and the help of stellar stylist Tara Swennen, Kristen Stewart is a red carpet pro — and we can't wait to see what high-fashion frocks she'll break out while promoting the highly anticipated flick, Breaking Dawn. Luckily, we won't have to wait until she comes down the carpet — Tara, her stylist, provided us with this key clue when she tweeted, "Met with some of my fave British designers-Erdem, Kirkwood, Pilotto etc-for KStew-Excited to get this ball rolling! Thx Starworks!" We pulled together some of the most recent looks from the designer's latest collections — could these possibly be the dresses we're going to see on the star?
A dress from Erdem's Spring 2012 collection.
A look from the Peter Pilotto Spring 2012 collection.
A dress from Peter Pilotto's Spring 2012 collection.
A look from Peter Pilotto's Spring 2012 collection.
A look from Erdem's Fall 2011 collection.
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Happy With You Skinny Jeans - Medium Wash
You plus these skinnies together, nothing better! These Happy With You Skinny Jeans in a dark wash feature a stretch fabric with a mid-rise, skinny jean styling. Thick waistband with triple button closure. Classic five-pocket design and finished hem. Push-up stitching enhances your assets and rounds the rest!
*Cotton/Polyester blend
*27" Waist; 38.25" Length; 30" Inseam
*Model is wearing size 1. (Measurements taken from size 1.)
*Hand wash cold
Size US Size US XXS 0 L 8 P/XS 2 XL 10 S 4 XXL 12 M 6
Height: 5'6"
Bust: 32" / Waist: 22" / Hips: 36"
Astrological sign: Cancer
Best thing ever? Modeling!
What's your little secret? I have never tasted sushi.
Email Us Call Us Click here to send email. US: 866-745-1800
Hours: Mon-Fri 9am - 5pm MST


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The Pilgrimage of Grace and the Politics of the 1530s
In 1536, Henry VIII faced uprisings first in Lincolnshire, then throughout northern England. The Pilgrimage of Grace posed the greatest threat encountered by any Tudor monarch. The risings have traditionally been assumed to be in protest to Henry's dissolution of the monasteries, but in this lively and colorful history, R. W. Hoyle uncovers the full story behind these dramatic and tragic events.
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Sunny B Hotel offers an airport shuttle (available on request) for VND 250000 per vehicle one way. Guests will have free perks like WiFi and self parking. Rooms offer up nice touches like jetted tubs and slippers, along with balconies and 24-hour room service.