text:
If there was no lockout, Kemba Walker would be making his home debut for the Bobcats on Friday. Instead, he's working out at UConn.
Kemba Walker would much rather be in Charlotte right now, playing his first pro games with the Bobcats.
Instead, he's hanging out in Connecticut, training with the UConn Huskies and waiting for the NBA lockout to end.
"It's cool," the Bronx-born Walker said last week, at an appearance to promote his new UnderArmor sneakers. "I'm working out with the team, practicing with those guys. I'm trying to help them get better and they're helping me get better."
Of course, he'd prefer to be working with the rest of his Bobcats teammates. But the NBA lockout has that on hold for now. So he's in Storrs - - playing alone in the morning and with the team later in the day - - helping ex-teammates like Shabazz Napier and Jeremy Lamb prepare for the season.
"It's great to me," Walker said. "Everything is free."
Walker was smiling when he said it, but it's clear that the lockout - - which entered its 127th day on Friday - - is starting to frustrate him.
"As time goes by, now it's starting to get harder because it gets a little old, working out every day alone," he said. "I'm trying to stay positive. I'm just ready to play basketball. That's the only thing, I'm ready."
The former Rice HS star left UConn last year after a fairy-tale junior season in which he earned Big East Tournament MVP and led the Huskies to the national championship. He also earned his degree in three years, so he decided to enter the NBA Draft early without much second thought.
Now, as he works out with his ex-teammates, Walker wonders what would have been if he'd stayed for his senior season.
"I wouldn't think about that if there was no lockout," he says.
If there was no lockout he'd be in Charlotte, getting ready for his first home game with the Bobcats on Friday. Instead, he's still working out in Stoors.
WALKER HONORS GUACHOS: Walker played on what many considered the top AAU team in the country in the summer of 2007. He shared a backcourt with future West Virginia guard Darryl Bryant and future Drexel guard Chris Fouch with the Guachos that summer. Also on that team was future Long Beach State forward Dan Jennings, future Seton Hall guard Jordan Theodore and future Miami guard Durand Scott.
To honor that edition of the Gauchos, which was coached by current Arizona assistant Emmanuel "Book" Richardson, Walker and UnderArmor created an orange and black sneaker featuring the names of his Gauchos teammates on the insole.
Most of his Gauchos teammates were products of New York City high schools as well. Walker, Scott and Fouch went to Rice HS in Manhattan and Bryant went to St. Raymond HS in the Bronx.
Making the shoe was "a dream come true," Walker said.
Walker's face is plastered on subway posters and the sides of buses all over New York City as part of UnderArmor's "Are you from here?" campaign to promote the shoe. He's also featured in a national commercial.
"It's crazy. I never thought I'd ever see something like that," he said.


text: Irene's doesn't fit into one category - it's a place where you could eat brunch, or dinner, or something kind of healthy, or just drink. When most restaurants try to be everything to everyone, they end up feeling like a schizophrenic off their meds. But at Irene's, it works. That said, while the food is solid, we like Irene's best for a morning toast and coffee, or an afternoon cocktail and a snack. The overall atmosphere lends itself better to hanging than it does for full-on meals.

subset: commoncrawl


text:
You missed it This great deal for Innovation Dermal Clinique is over. But don't despair, we've got more great deals every day.
We're turning three! Celebrate with one of our favourite local businesses of the last three years:
We all have those lightbulb moments, but it's about time the power of actual light was recognised. If you're a bright spark and it's hair removal you're after, then enlighten yourself with today's deal from Innovation Dermal Clinique -- six IPL hair removal sessions on two areas for £89 (up to a £1,440 value).
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text:
A Pistol for the King
Lazangan's tail, the main part of it, blocked the incoming attack. Because this spiral power did not outstrip his own, it wasn't hard to block. The only thing he hadn't accounted for was the sheer power of the enemy mech. Damn puny children.
Lordgenome thought of a plan as Lazengann slowly slid backwards, etching a long trench in the earth. The other rebels were escaping, but it mattered not. With Perceptual Teleportation, Lordgenome had already set up an ambush for the rebels. They would be met with nearly half of Lordgenome's forces, which still tripled what the rebels had. He had also sent a special beastman, named Timult, to help with the ambush. It would take a lot to stop that one.
"To answer your question," Lordgenom said, turning and letting the faceless gunman fly by. It's spiral power was nearly depleted. Well, that was expected. Had the fool had any spiral power, it would have been apparent to another spiral warrior. No one alive had any substantial spiral power. All the spiral warriors were dead. These rebel children would be as well, soon. "Perceptual Teleportation can be activated at any time for any of my beastmen, so long as I have the spiral power to do so. Earlier I had my forces teleport as well as teleport myself."
With that he jumped into the air and pulled a pistol. "I always hated using these things," Lordgenome said to no one in particular, "But these railguns are quite useful." He pointed the pistol towards his enemy. It powered up and shot instantly.


text:
best of craigslist > portland >
Did you leave a prosthetic leg on the bus this morning? - w4m
I found a prosthetic leg wearing a Lucchese brand cowboy boot on the bus this morning. I'm not sure what brand the leg is, but it's only the shin and foot portion and it's not mechanical looking.
Rather, it looks semi-real--almost like it could be from a giant plastic doll or something. Here's a sketch.
I hope you don't mind, but I went ahead and tried the boot on. Magnificent!
At first I wondered why a prosthetic leg user would have purchased such a comfy boot. Then I figured that you probably have another leg and foot that actually work and they probably really appreciate the cushion and arch support provided by the Lucchese boots.
I really hope I can meet you and ask you more about your condition.
I wonder what Koreans would say about you if you went in for a pedicure. Do you get charged half price for pedicures? That would be fucked up if you didn't.
Do you think they'd charge me full price if I went in for a pedicure with you?
(Read more)


text:
- Vitamin Guide
- Health Conditions
- Health Centers
- Diet & Weight Loss
- Herbal Remedies
- Current News
- Food Guide
- Recipes
Jet lag is a disturbance of the sleep-wake cycle triggered by travel across time zones.
Jet lag causes a combination of symptoms, including daytime sleepiness, disorientation, poor concentration, fatigue, gastrointestinal discomfort, headaches, difficulty falling asleep, and frequent waking from sleep. The symptoms can last from a day to a week or longer, depending on the person and the number of time zones crossed.
Copyright © 2017 Healthnotes, Inc. All rights reserved. www.healthnotes.com
Learn more about Healthnotes, the company.
The information presented by Healthnotes is for informational purposes only. It is based on scientific studies (human, animal, or in vitro), clinical experience, or traditional usage as cited in each article. The results reported may not necessarily occur in all individuals. Self-treatment is not recommended for life-threatening conditions that require medical treatment under a doctor's care. For many of the conditions discussed, treatment with prescription or over the counter medication is also available. Consult your doctor, practitioner, and/or pharmacist for any health problem and before using any supplements or before making any changes in prescribed medications. Information expires December 2017.


text:
Welcome to MotoHouston.com! You are currently viewing our forums as a guest which gives you limited access to the community. By joining our free community you will have access to great discounts from our sponsors, the ability to post topics, communicate privately with other members, respond to polls, upload content, free email, classifieds, and access many other special features. Registration is fast, simple and absolutely free, join our community!
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Astros Opening Day festivities at Minute Maid Park
Collin McHugh (0-1) starts Houston's home opener Monday against the Kansas City Royals in a rematch of the 2015 AL Division Series.
McHugh took the loss in the Royals' clinching Game 5 last season, allowing three runs in four innings. He will be opposed by Chris Young.
Monday's game is sold out and fans are encouraged to wear orange in an "Orange Out" of the ballpark.
The Opening Day Street Fest runs from 2 p.m. through 6:30 p.m., with first pitch scheduled for 7:10 p.m.
This year's Street Fest features live music, food trucks, games, and appearances by Astros manager A.J. Hinch, general manager Jeff Luhnow, pitcher Mike Fiers and other 'Stros players, along with H-town rapper Bun B.
Before the game, Dallas Keuchel and Carlos Correa will receive their 2015 AL Cy Young and AL Rookie of the Year awards, respectively. Astros' legends Craig Biggio and Roger Clemens will make a special appearance.
If you're going to the game or you're just decked out in your Astros' gear, we want to see your photos! Send them to email@example.com.
And you can view your photos here.
There are rain chances for Monday afternoon, so make sure to check the weather forecast here or download Frank's Forecast app.
Here's the home opener schedule below:
2 p.m. - 6:30 p.m. - Opening Day Street Fest on Crawford Street
3 p.m. - 3:15 p.m. - Astros pep rally with A.J. Hinch and Jeff Luhnow, emceed by Steve Sparks
3:30 pm. - 3:45 p.m. - Astros pep rally with Astros players, emceed by Steve Sparks
4 p.m. - Ballpark gates open
5:30 p.m. - 5:45 p.m. - Bun B performance on Budweiser Stage
6:30 p.m. - Opening Day on-field ceremonies
7:02 p.m. - National Anthem performed by Clay Walker
7:10 p.m. - First pitch HOU vs. KC
7th inning stretch - Bless America by Neal McCoy
|Thread Tools


text:
Health Insurance industry is going through a rough phase of a decline in revenue in last few years. Even new attractive schemes are not able to revive the industry. The reason for slow down can be solely attributed to the sub-standard services offered by the company. However, now IRDA (Insurance Regulatory and Development Authority) has proposed a draft regulation which if approved, then it will provide excellent services to the policy holders. Most of the problems are tackled in such a way that it will benefit to a policy holder in most of the cases. The draft has answers to lots of problems related to servicing issue such as timely claim settlement, furnishing reason for claim rejection and fixing clear accountability of the insurance companies for all the actions pertained to them.
Following are some important points covered in the draft:
Earlier, insurer used to withdraw the schemes that were loss making for them by shifting the policy holders to a new scheme. The insured used to compromise with the insurance companies' decision. Now, the draft proposal has suggested that insurer has to continue with the old scheme if the policyholder doesn't want to shift to new one. IRDA has said, "Insurer shall not compel the insured to migrate to other health insurance products, if it is to the disadvantage of insured."
These proposed changes would not only give more business to the insurance companies but also help the policy holders to opt for a tension-free treatment. However, Insurance companies have to tackle with another challenge that would be to provide disclosure containing coverage and estimated premium for future renewals of the policy. Insurers would be submitting their response to the General Insurance Council (association of non-life insurers) to place the matter in front of IRDA and it is expected that most of the proposals would be accepted in favor of policy holders. The draft regulations on health insurance released by IRDA have plugged the various loopholes through which customers are mistreated by insurance companies.
Amit Sethi is an MBA (Fin) graduate and a Financial Consultant. He has spent 9 years in Equity research, Stock broking and Financial Consultancy Sector. He can be reached at email@example.com
Insurance Calculator


text:
Paul Pierce, Doc Rivers Credit Bench for Keeping Celtics Stars Rested, Rejuvenated During Recent Run
Originally posted on NESN.com | Last updated 1/12/13
BOSTON — Paul Pierce and Kevin Garnett had gotten used to running damage control. Their task, during the first two months of the season, often was to hand the Celtics' bench a workable lead and pray that the score stayed manageable while they sat, so they could reclaim the lead later. The approach did not always work, as their dreadful December and five-game losing streak entering the new year attest. When Celtics coach Doc Rivers insisted the problems could be fixed, he was met mostly with blank, incredulous stares. Now the Celtics are doing everything their coach predicted they could, and more. The second unit that was such a drain has been reborn, as the return of Avery Bradley to the starting lineup has secured the lineup rotations. The "scary deep" squad Pierce tweeted about in preseason truly has been frightening for opponents to face. "They've been huge for us during this winning streak," Pierce said after the bench combined for 40 points in 93 minutes in a 103-91 victory over the Rockets to run their streak to five straight wins. "They've been coming in and been able to not only sustain leads, but come in and push leads. I come out of the game and we're down three or four, and I come back in and we're up 10. That's a lot of pressure off me and the rest of the starters when you've got a bench playing so confident, playing great defense and moving the ball." Once again, led by the bench, the Celtics out-hustled an opponent en route to a win. In a stirring second-quarter flurry, the unit of Jared Sullinger, Courtney Lee, Jason Terry, Jeff Green and Garnett seemed to grab every rebound and track down every loose ball. Terry and Green got floor burns gobbling up loose balls that turned into Celtics baskets, while Sullinger piled up eight points and five of his game-high 11 rebounds. When Pierce exited the game with a little over three minutes to go in the first quarter, the Celtics trailed by five. When he re-entered with four minutes remaining in the second quarter, they led by 11 points. Not even Pierce's 23 points were as telling as that statistic. The key aspect to the bench's performance of late is more than pure adrenaline, however. Boston's reserves now appear to trust each other as much as the starters, who teamed up to play all the major minutes in the Celtics' run to the Eastern Conference Finals last season. For a solid 12 minutes of game action, the backups moved in even greater unison than Rajon Rondo, Brandon Bass, Garnett, Pierce and Bradley had to begin the game. "We've decided to defend as a group full-time," Rivers said, "and stay committed to it. That team-wide effort held James Harden to 24 points, marking the first time in 14 games that he has failed to reach at least 25 — the longest such streak in franchise history. But the defense did more than halt a relatively meaningless individual statistical streak. If not for the second unit's performance, certainly Harden might have gotten his 25 points, but more importantly the Celtics might not have come out with a victory. Pierce and Garnett may not have had the juice to carry them down the stretch. The Rockets should have been finished early in the fourth quarter when Garnett completed a three-point play to put Boston ahead by 14 points. Instead, the Rockets continued to chip away. Once Chandler Parsons converted a bad pass by Rondo into a breakaway dunk, Houston trailed by only two points with more than seven minutes remaining. The fans at the TD Garden were justifiably concerned. As it turned out, those fans had little reason to worry, because Pierce and Garnett were present and fresh. Pierce came out of the Boston timeout to hit a step-back jump shot. Two possessions later, after two free throws by Rondo and a putback dunk by Bass, Pierce picked off a pass by Harden and barreled down the court for an and-one layup to extend the lead to double-digits again. Garnett then put the game out of reach with a personal 6-2 run, which he punctuated with a hop-step slam that brought to mind his days as a vicious dunker with the Minnesota Timberwolves. It is possible that none of those big shots would have happened, however, if not for the bench's spurt earlier in the game. Thanks to the run and the extended rest it afforded several of the starters, Pierce was able to stay fresh and ready to take over in crunch time. Extra time on the bench is invaluable for a star who is only 35 in human years, but is pushing Social Security eligibility in NBA years. "They're saving us," Garnett said. "Let's all be real. The bench is saving us. Everybody that's coming off the bench is giving us great minutes." The reserves saved the starters early, so the starters could save the game late. This sort of division of labor is much easier for Pierce and Garnett to handle, and has led to an exponential improvement in workplace morale. Have a question for Ben Watanabe? Send it to him via Twitter at @BenjeeBallgame or send it here.
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text:
Park Funding Problems? Privatize
Stossel looks at New York City's Bryant Park, which has been privatized.
"Sounds good to me. But not to Shirley Kressel, a Boston journalist.
I asked her what's wrong with getting the money from private businesses, as Dan does.
'Because it goes into private pockets,' she said.
So what?
'Because it's very good (for Dan) to use the public land for running a private business, a rent-a-park, where all year 'round there's commercial revenue from renting it out to businesses. He keeps all that money. People don't realize that.'
So what? I don't care if they think the money is going to Mars. The park is nice, and people don't have to pay taxes to support it."


text:
This post is the first in the series of Search Engine Marketing Tips with Webrageous Studios and aims to focus your attention on the use of linkbaiting as an effective search engine marketing technique.
Firstly, what is linkbaiting? If you are unsure what linkbaiting is, then you can read a full explanation into the subject via the Webrageous Studios website. If already fully aware of the concept of linkbaiting, the question is how does linkbaiting function as an effective form of search engine marketing?
The answer is that if you are creating articles that are linkbait worthy, the content of those articles is going to be good. Once these content rich articles are on your website, the authority and usefulness that they will provide for all internet users means that your site begins to be one that internet users want to return to time and time again.
For example, linkbait articles that are full of lists with hyperlinks to various different resources of high quality around the web are attractive to internet users. Anything that gives an internet user a one-stop answer to a number of related topics and subject areas is excellent linkbait, excellent traffic driving material and therefore an excellent example of search engine marketing genius.
If you want to improve your search engine marketing, then Webrageous Studios´ top tip number 2 in the series of three is to become proficient in generating linkbait or to employ someone else to do it for you.
Take a look at the full length article posted on the Webrageous Studios Website which lists a number of different ways in which to create and utilize linkbait worthy articles in general and then maybe take the time to read the article we have posted about the way in which to utlize sites like Digg.com to improve your search engine marketing campaign too.


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text:
PURPOSE: Sepsis is associated with higher morbidity, mortality and health care cost in the US. Limited data are available regarding the impact of sepsis in elderly patients with immunosuppression. Therefore, our aim was to determine the impact on mortality for patients with immunosuppression that develop severe sepsis.
METHODS: A retrospective administrative data of hospitalized inpatients aged >/= 65 years with a discharge diagnosis of sepsis (by ICD-9 codes 0.38 and 0.20) in fiscal years 2002-2007, and at least 1 year of Department of Veterans affairs outpatient care before the index admission were included. Severe sepsis was defined as sepsis with at least one organ failure. Immunosuppression was constructed as a comprehensive variable that included active malignancy, immunosuppressive therapies and diagnosis (e.g. HIV/AIDS, medications, neutropenia, etc). Primary outcome was 30-day mortality. Chi-square and Student's t-test were used to compare categorical and continuous variables, respectively. Multivariable analysis was performed using 30-day mortality as the dependent variable and independent variables that were p <0.1 in the bivariate analysis.
RESULTS: Our results showed that severe sepsis was present in 9,139 subjects. The most common immunosuppressive conditions included: any type of cancer (25%), solid organ tumor (27%), and corticosteroid therapy (22%). Multivariable analysis (Odds ratio [OR], 95% Confidence Intervals [CI]; p value) showed that age (OR=1.03; 95%CI 1.03-1.04; p<.001), mechanical ventilation (OR=1.23; 95%CI 1.13-1.34; p<.001), lung metastasis (OR=1.34; 95%CI 1.13-1.60; p=.001), neutropenia (OR=1.53; 95%CI 1.15-2.03; p=.003), and cisplatin therapy (OR=2.21; 95%CI 1.07-4.58; p=.03) were associated with higher 30-day mortality. In contrast the use of corticosteroid therapy was associated with lower 30-day mortality (OR=0.62; 95%CI .56-.69; p<.001) in severe septic patients.
CONCLUSIONS: Elderly patients with severe sepsis and immunosuppression, lung metastasis, neutropenia and cisplatin therapy are associated higher 30-day mortality. In contrast corticosteroid therapy is associated with lower 30-day mortality.
CLINICAL IMPLICATIONS: Further prospective studies are needed to evaluate the impact of sepsis in patients with immunosuppression.
DISCLOSURE: The following authors have nothing to disclose: Juan Fernandez, Andrew Shorr, Eric Mortensen, Antonio Anzueto, Marcos Restrepo
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text:
The past week's selection of (mostly) science-related blog carnivals:
All Things Eco Blog Carnival Volume Twenty
Brain Blogging, Fortieth Edition
The 41st Carnival of Mathematics
Carnival of Space no. 74: the Orion Arm, Vladimir Bonaparte and Watermelon Planet edition
Gene Genie 38: Back in action!
Medicine 2.0 Blog Carnival #34


text:
Folate metabolite profiling of different cell types and embryos suggests variation in folate one-carbon metabolism, including developmental changes in human embryonic brain
- First Online:
- Received:
- Accepted:
DOI: 10.1007/s11010-013-1613-y
- Cite this article as:
- Leung, KY., De Castro, S.C.P., Cabreiro, F. et al. Mol Cell Biochem (2013) 378: 229. doi:10.1007/s11010-013-1613-y
- Citations
- 1.3k Downloads
Folates act as co-factors for transfer of one-carbon units for nucleotide production, methylation and other biosynthetic reactions. Comprehensive profiling of multiple folates can be achieved using liquid chromatography tandem mass spectrometry, enabling determination of their relative abundance that may provide an indication of metabolic differences between cell types. For example, cell lines exposed to methotrexate showed a dose-dependent elevation of dihydrofolate, consistent with inhibition of dihydrofolate reductase. We analysed the folate profile of E. coli sub-types as well as cell lines and embryonic tissue from both human and mouse. The folate profile of bacteria differed markedly from those of all the mammalian samples, most notably in the greater abundance of formyl tetrahydrofolate. The overall profiles of mouse and human fibroblasts and mid-gestation mouse embryos were broadly similar, with specific differences. The major folate species in these cell types was 5-methyl tetrahydrofolate, in contrast to lymphoblastoid cell lines in which the predominant form was tetrahydrofolate. Analysis of embryonic human brain revealed a shift in folate profile with increasing developmental stage, with a decline in relative abundance of dihydrofolate and increase in 5-methyl tetrahydrofolate. These cell type-specific and developmental changes in folate profile may indicate differential requirements for the various outputs of folate metabolism.
KeywordsFolate Embryo Methotrexate Bacteria Liquid chromatography tandem mass spectrometry
Abnormalities in FOCM have been implicated in a variety of pathological conditions including cancer, neural tube defects (NTDs), cardiovascular disease, anaemia and neurological conditions [3, 8, 9, 10, 11]. Notably FOCM is implicated in diseases that may occur throughout life, including birth defects that arise during early development. For example, maternal supplementation with folic acid reduces the risk of NTDs whilst sub-optimal folate status and elevated homocysteine are associated with increased predisposition to an affected pregnancy (reviewed in [9]). The observation of defective thymidylate biosynthesis in some human NTD cases and mouse models [12, 13, 14] supports the hypothesis that altered FOCM may contribute to development of NTDs. Folate status has also been investigated in relation to risk of several different cancers, colorectal cancer being probably the most intensively studied. Epidemiological studies have shown an association of low folate intake with risk of colorectal adenomas and cancer, and use of folic acid supplements has been reported to reduce risk and mortality. However, folic acid supplements may also promote progression of pre-existing preneoplastic lesions, thereby increasing cancer risk [10, 15]. A similar non-linear relationship, in which both low and high folate intake are associated with risk, has also been reported for postmenopausal breast cancer [16].
The multiple outputs of FOCM imply several different biochemical mechanisms by which impaired function may influence embryonic development and post-natal pathologies. Nucleotide biosynthesis is essential to support DNA replication and cell proliferation, which may be of particular relevance to embryonic development, whilst impaired thymidylate biosynthesis causes an increase in erroneous incorporation of uracil into nuclear DNA [1]. The supply of methyl groups is also required for cellular methylation reactions and may impact DNA methylation, possibly leading to altered transcriptional regulation. In this context, there is increasing evidence of a potential link between FOCM and the foetal epigenome, although whether altered methylation contributes to birth defects has yet to be established [17].
Alterations in FOCM can be investigated by analysis of downstream biomarkers such as homocysteine concentration, DNA methylation and uracil incorporation [1]. Mathematical modelling also allows predictions of the effects of folate status or enzyme inhibition on methylation and nucleotide biosynthesis [18, 19]. It will also be informative to directly quantify FOCM intermediates to evaluate possible alteration in the ratio of abundance of individual metabolites that may reflect disturbance of a particular step(s). For example, 10-formyl-THF provides one-carbon units for purine biosynthesis and a reduced abundance of this metabolite, relative to its precursor THF, could lead to diminished purine synthesis. Similarly, alteration in the relative abundance of 5-methyl THF may be informative about the contribution of methyl groups to the methylation cycle.
Folates are transported into cells in the monoglutamated form and multiple glutamate residues are then added by the action of folylpolyglutamate synthetase. Polyglutamation is required for cellular retention of folates and an optimal analytical approach should therefore distinguish these forms. Previously, we used liquid chromatography tandem mass spectrometry (LC–MS/MS) for quantification of s-adenosyl methionine (SAM) and s-adenosylhomocysteine (SAH) in order to identify perturbations in the methylation cycle [20, 21]. In the current study, we applied LC–MS/MS to analyse FOCM intermediates in bacteria and for comparison of human and mouse cell lines and embryonic tissue, which have not previously been subject to comprehensive analysis.
Materials and methods
Folate standards including dihydrofolate (DHF), tetrahydrofolate (THF), 5,10-methylene THF, 5-formyl-THF, 5,10-methenyl THF, 5-methyl THF, folic acid (PteGlu), PteGlu3, PteGlu4, PteGlu5, PteGlu7 and methotrexate were purchased from Schircks Laboratories (Switzerland). Remaining reagents were methanol, acetonitrile (Fisher Scientific, UK), ammonium acetate, ascorbic acid, DTT, citric acid (Sigma-Aldrich) and N,N-dimethylhexylamine (Fluka).
Samples for analysis
Bacteria OP50 and HT115 Escherichia coli strains [22, 23, 24] were grown overnight in LB from a single colony at 37 °C. NGM plates [24] were seeded with 150 μL bacterial suspension and incubated for 96 h at 20 °C. Bacterial lawns were washed from the plates using M9, collected by centrifugation at 4 °C, 4,000 rpm for 20 min and the bacterial pellet stored at −80 °C prior to analysis. Cell lines EBV-transformed human lymphocytes were collected with ethical permission from normal Swedish individuals (Karolinska Institutet). Cells were cultured in RPMI 1640 media with 10 % FCS. For LC–MS/MS analysis, 2 × 107 cells were harvested, washed in PBS and cell pellets stored at −80 °C prior to sample preparation. Human tissue was obtained as frozen samples (at −80 °C) from the human developmental biology resource (www.hdbr.org). Mice wild-type (CBA/Ca and C57BL/6) strain mice were mated and mouse embryos were collected at embryonic day (E) 12.5 [25]. Embryos were immediately frozen on dry ice and stored at −80 °C.
Sample preparation
Buffer containing 20 mM ammonia acetate, 0.1 % ascorbic acid, 0.1 % citric acid and 100 mM DTT at pH 7 was added to cells, tissues or embryos. Where quantitation was performed, 3 μl of 10 μM methotrexate was added as internal standard. Sample suspensions were sonicated for 10 s using a hand-held sonicator at 40 % amplitude. A 10 μl aliquot of each of the homogenised samples was removed for DNA quantification using NanoDrop (thermo scientific). Protein was removed by precipitation by addition of 2 sample volumes of acetonitrile, mixing for 2 min and centrifugation for 15 min at 12,000×g and 4 °C. Supernatants were transferred to fresh tubes, lyophilised and stored at −80 °C prior to analysis.
Lyophilised samples were resuspended in 50 μl water (milli-Q) and centrifuged for 5 min at 12,000×g at 4 °C. Supernatants were transferred to glass sample vials for LC–MS/MS analysis. Metabolites were resolved by reversed-phase chromatography (Luna C18 column; 150 × 2.0 mm2; 5 μm bead size; Phenomenex, UK) using a 2795XE high performance liquid chromatography unit with solvent divert valve (Waters Corporation, UK). Solvents for HPLC were: Buffer A, 5 % methanol, 95 % Milli-Q water and 5 mM dimethylhexylamine at pH 8.0; Buffer B, 100 % methanol, 5 mM dimethylhexylamine. The column was equilibrated with 95 % Buffer A: 5 % Buffer B. The sample injection volume was 40 μl. The HPLC protocol consisted of 95 % Buffer A: 5 % Buffer B for 1 min, followed by a gradient of 5–60 % Buffer B over 9 min and then 100 % Buffer B for 6 min before re-equilibration for 4 min. The metabolites were eluted at a flow rate of 200 nl/min. The HPLC was coupled to a triple quadrupole tandem mass spectrometer (MicroMass Quattro, Waters Corporation, UK) operating in negative-ion mode using the following settings: capillary 3.54 kV, source temperature 150 °C, desolvation temperature 350 °C, cone gas flow rate 25 l/h and desolvation gas flow rate 950 l/h. Folates were measured by multiple reaction monitoring (MRM) with optimised cone voltage and collision energy for precursor and product ions (based on [26]).
Data analysis and statistics
The peak areas of individual folates were extracted using MassLynx software (Waters). The total peak area of each folate (sum of mono- and polyglutamated forms) was expressed as a percentage of the total folate in each sample. Data were analysed using SigmaStat (Systat Software, Version 3.5). Pairwise comparisons were made by t test, and multiple comparisons by one way ANOVA with Holm-Sidak post hoc test.
MRM values for folate standards
MRM transition (m/z)
5-Formyl THF
472.17 > 314.95
5-Methyl THF
458.19 > 328.89
5,10-Methylene THF
456.28 > 326.98
5,10-Methenyl THF
454.00 > 281.00
444.20 > 314.85
441.97 > 175.99
Folic acid: mono and polyglutamates
PteGlu (folic acid)
440.05 > 310.86
698.37 > 421.99
827.52 > 422.02
956.67 > 422.03
606.84 > 422.02
In contrast to comparisons between fibroblasts and mouse embryos, the folate profiles of these cell and tissue types differed markedly from those observed in lymphoblastoid cell lines. The principal difference was the predominance of 5-methyl THF as the major folate species in fibroblasts and embryos, compared with THF in lymphoblastoid cells (Fig. 2b, c).
In addition to comparison of the total abundance of each major folate, we also analysed glutamation patterns. In bacterial samples, multiple glutamated forms of each folate including all types from Glu2 to Glu7 were present in varying proportions (Fig. 3a shows 5-methylTHF in OP50 strain as a representative example). The most abundant polyglutamated form in bacteria was somewhat variable between different folates, but in general corresponded to Glu3, Glu4, Glu6 and Glu7. The Glu2–Glu7 forms of 5-methyl THF were also detected in mouse embryos, the major forms being Glu5 and Glu6 (Fig. 3b). However, in contrast to bacteria, the monoglutamate also made up a sizeable proportion (~20 %) of 5-methyl THF in embryos. Analysis of the glutamation state of several folates suggested that abundance of monoglutamate is principally a feature of 5-methyl THF, as the predominant forms of the other folates were the 5–7 glutamated forms (Fig. 3c).
Folate one-carbon metabolism plays a fundamental role in key cellular functions including nucleotide biosynthesis and methylation and is implicated in a number of diseases. Comprehensive profiling of folates within a cell or tissue type will provide an opportunity to investigate disturbance of metabolism and potentially to focus attention on particular step(s) that may be abnormal in a disease state or following treatment with pharmacological agents. Moreover, folate profiling will provide opportunities to investigate the metabolic effects of targeted mutation of FOCM enzymes in model systems. This may include loss-of-function alleles, a number of which have already been generated in mice, as well as specific alleles designed to mimic putative disease-causing mutations in humans.
The sensitivity of LC–MS/MS profiling to detect gross disruption of FOCM was tested by exposure of lymphoblastoid cells to methotrexate, a DHFR inhibitor. As predicted, the level of DHF was markedly increased in treated cells, a finding which parallels the elevation in DHF levels observed in lymphoblasts derived from patients with inherited DHFR deficiency [29].
LC–MS/MS methodology has previously been used for simultaneous profiling of folate metabolites in samples from different plant species [26]. In the current study, we used an LC–MS/MS approach to perform comprehensive folate profiling in bacterial sub-types as well as mouse and human cell lines and embryonic tissue. Each of the analysed folates was detectable in each of the different types of samples but we observed notable variation in the relative abundance of the different folate metabolites. Perhaps unsurprisingly, the overall folate profile substantially differed between bacterial and mammalian samples, in particular with a greater proportion of formyl-THF in bacteria. These differences may reflect, in part, the variation in FOCM enzymes in bacteria and mammalian cells. In particular, in eukaryotes, the trifunctional enzyme MTHFD1 (methylene tetrahydrofolate dehydrogenase/methylene tetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase) catalyses the interconversion of 5,10-methylene THF, 5,10-methenyl THF and 10-formyl-THF [1, 2]. However, in bacteria, these reactions are catalysed by two or three separate enzymes with conversion of THF to 10-formyl-THF by a monofunctional synthetase enzyme. In addition to possible variation in regulation of FOCM, differences in folate profile likely reflect the fact that, unlike mammalian cells, bacteria can synthesise folates de novo.
The overall folate profile patterns were similar in human and mouse fibroblast cell lines and mouse embryos, despite differences in the relative abundance of specific folate metabolites. In contrast, lymphoblastoid cell lines exhibited a rather different profile, with a significantly higher THF and lower 5-methyl THF than in fibroblasts. The metabolic basis of this variation in profiles has not been defined but computational modelling indicates that reduced activity of MTHFR or elevated activity of methionine synthase in lymphoblasts compared with fibroblasts could result in the observed difference in profile. The functional significance of differing profiles could be hypothesised to reflect the metabolic requirements of different cell types. For example, diminished MTHFR activity could indicate channelling of one-carbons towards nucleotide biosynthesis at the expense of methylation in lymphoblasts.
We also observed developmental shifts in folate profile in human embryonic brain, with an overall decline in DHF and THF and a corresponding increase in 5-methyl THF during the period from around 6 to 9 weeks of gestation. This change correlates with the onset of neuronal migration in the primate embryonic brain [30] and could potentially be effected through increased activity of MTHFR (or reduced activity of methionine synthase) with development. Increased availability of 5-methyl THF could potentially support methylation reactions at later stages as the overall behaviour of the cellular population shifts from proliferation towards differentiation.
In recent years, it has become increasingly clear that FOCM is compartmentalised at the sub-cellular level and that this is likely to be functionally important in regulation of flux through different pathways [2, 31]. For example, enzymes required for de novo thymidylate biosynthesis (SHMT1, TYMS and DHFR) localise to the nucleus during S and G2/M phase of the cell cycle [31]. Folates must, therefore, be present in the nucleus to supply one-carbon units for dTMP synthesis. Mitochondrial folate metabolism plays a crucial role in provision of one-carbon units as formate, to the cytoplasm (and nucleus). Thus, the majority of one-carbons used for nucleotide biosynthesis and methylation are derived from serine, glycine, dimethylglycine or sarcosine in mitochondria [2]. Mitochondrial-specific FOCM-related enzymes include MTHFD1L and MTHFD2L and the glycine cleavage system (GCS). Mutations in GLDC or AMT, encoding components of the GCS, cause non-ketotic hyperglycinemia and predispose to NTDs [32, 33]. The identification of diseases associated with FOCM in specific sub-cellular compartments suggests that fractionation prior to LC–MS/MS analysis will be important in evaluating the metabolic consequences of putative causative mutations. Comprehensive metabolite profiling, in combination with predictive modelling, may then provide a useful tool to investigate downstream functional effects on FOCM and indicate possible routes to therapeutic intervention.
The authors are grateful to Kevin Mills and members of ICH Biological Mass Spectrometry Facility for helpful discussion and advice. This study was supported by Sparks [08ICH03], the MRC [J003794] and the Wellcome Trust [Grant No. 087525). The human embryonic and foetal material was provided by the Joint MRC (Grant No. G0700089)/Wellcome Trust (Grant No. 082557) Human Developmental Biology Resource (http://hdbr.org).
Supplementary material
Copyright information
Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.


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HEAD Introduces New Performance Tennis Footwear
HEAD is stepping up its game in the tennis footwear business. In 2009 HEAD will re-launch its line of footwear with updated technology and cosmetics for today's youthful, aggressive and stylish tennis player. The new footwear collection is available since January 2009.
Both men's and women's lines' technology will complement existing product attributes, continue to enhance and improve player performance on any court surface while adding updated cosmetic and asthetic looks for the modern athlete.
The 2009 line incorporates three new lines of technology:
- HEAD Vi-Pod™ Technology, which is integrated into the midsole heel area and eliminates vibration, absorbs energy, then distributes to the entire foot.
- HEAD Energy Bridge™ Technology, which connects the heel and the forefoot area providing torsion stability, ensures increased transfer of power from the heel to the forefront. The Power Bridge is made of breathable net mesh material to ensure ventilation.
- HEAD Lateral Control™ Technology is integrated into the forefoot area with a squared edge. It is composed of a harder rubber which provides an optimum in lateral stability and enables quick changes in direction.
"HEAD's line of footwear products are constructed with the highest quality materials while maintaining cutting edge design. More importantly, the shoes are made to perform. The footwear can stand up to even the most aggressive tennis player," said Robert Marte, Executive Vice President of HEAD's Racquet Sports Division.
These new technologies will add to the line's four existing technologies, which include:
- HEAD CXG™ Comfort Grid which provides comfort, shock absorption, decreased foot fatigue, stability and motion control.
- HEAD Cooling System® which creates "climate control" within the shoe by absorbing and releasing excess heat build-up.
- The HEAD Radial Contact™ ensures constant traction with maximum flexibility.
- The HEAD HyBrasion™ outsole technology was developed to enhance resistance for the high stress areas of the tennis shoes.
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Job Opportunity : Research Assistant in Veterinary Epidemiology
Applications are invited from suitably qualified Tanzanians to fill a vacant position in the Intermediate Fellowship in Public Health and Tropical Medicine project funded by Wellcome Trust, UK, based at Sokoine University of Agriculture. Read more >>
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Best unit and overal winner of Website Ranking in the Sokoine University of Agriculture
University wide Website Committee (UWC) in its 11th meeting held on 7th March 2017 approved the results of ranking of university website for the College/School/Faculty/Department/Institute/Directorate and Centres website for the first quarter(January - March) year 2017.
Congratulations to the following units for winning and being the best College/School/Faculty/Department/Institute/Directorate and Centres website for the first quarter year 2017.
1. Sokoine National Agricultural Library
2. College of Forestry, Wildlife and Tourism
3.College of Agriculture (CoA)


text:
A Race Car Driver Took His Three-Year-Old with Him, and the Faces the Kid Made Are Priceless [Video]
A video posted in November is making the rounds . . . that shows a Russian race car driver taking his three-year-old SON for a ride. And from the look on the kid's face, he LOVED it, especially when they went around turns.
Some people say it's child endangerment, even though they were on a closed course.
Kane is joined by co-hosts Riley, Rose, Intern John and producer Erick. The high-energy, fast-paced program features music, conversations on the latest news and hot topics, Hollywood news, contests, call-in interaction with listeners, plus interviews with the biggest celebrities in music and pop culture. The show features signature segments such as "War of the Roses," "Hollywood," "The Tea," "John's Justice," "Ghosted" and "Moron Madness."
Kane Cares! We'd love to hear about your event or organization. Use this link to send us your information!


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Luminoth Temple (Disambiguation)
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Luminoth Temple may refer to two forms of media related to Metroid Prime 2: Echoes:


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Get Ready for an All New Cast of 'FAST MONEY'
CNBC's "Fast Money" just hasn't been the same since host Dylan Ratigan and contributor Jeff Macke left the show. The Masters have been pondering who could replace them and what other hosts would investors like to see on the show? The answer is simple, the new show should be hosted by the all knowing Papa Smurf, he can finally get the contributors under control. To replace Macke it's the simple choice of Fred Flintstone and to heat it up, add in Skeletor and someone who they can all push around -- Beaker.
The new Anchor for "FAST MONEY" -- Papa Smurf
There is just no other choice than Papa Smurf that can control a rambunctious cast of characters, he's been all over the smurfs and keeping them in-line for hundreds of years. Papa Smurf is gentle, wise, and knowledgeable which is why he's needed to bring back "FAST MONEY" to the masses. He serves as the Smurfs' full-time leader, but after the Bell closes the paternal figure of the Smurfs applies his well-being and harmony to the much needed cast of 'Fast Money'. Besides, how great would it be to hear Papa getting the crew to shut-up before they break for a commercial and throwing in "we'll be right back, after these Smurfy messages." Fast Money Trading Name - "The Blue One".
The new Jeff Macke -- Fred Flintstone
The man blows his top any time you mention Slate Rock, Caterpillar (NYSE:CAT), or Deere & Company (NYSE:DE) and can argue with the best of them.You need a man's man to throw around Pete Najarian and Flintstone's terrible temper might finally get Guy Adami to take a knee when guests are trying to make their point.
Finally the show will add the much needed 'blue colar' expertise to 'FAST MONEY' and with his bonus knowledge of bowling, playing pool, poker, lounging around the house, and playing golf -- he's a big win. Fast Money Trading Name - "The Twinkle Toes". Yabba-Dabba-Doo!
New Contributor -- Skeletor
Evil has never had a better enthusiast for short selling and bear markets than the "Evil Lord of Destruction" Skeletor. The Bears and Shorts of the market have a new hero and cheerleader to admire, Skeletor will represent the true evil and downside of Mr. Market. By the Power of Greyskull my ass, not on his watch, Tim Seymour you can run, but you can't hide from Skeletor's nasty ways. Fast Money Trading Name - "The Bone Face"
Guest Contributor -- Beaker
Finally someone that Karin Finerman can set on fire (literally) and everyone will laugh. There's no better guy to hit in the nose and get away with it than Beaker. Besides being shy, Beaker can only speak in "Meeps", so if you get sick of listening to everyone else on "Fast Money", you can look forward to Beaker's segment -- one word can go a long way. Fast Money Trading Name - "The Sucker Punch"
Happy Safe Investing Masters.
Here's the pic again of the all new Fast Money cast without theStockMasters.com logo in it:
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text:
Nance Carpet cited for exposing employees to amputation, shock
There are 21 comments on the Calhoun Times story from Jun 18, 2012, titled Nance Carpet cited for exposing employees to amputation, shock. In it, Calhoun Times reports that:
Nance Carpet and Rug Co. Inc., a company that has been in Calhoun for several decades, has recently be noted by the U.S. Department of Labor's Occupational Safety and Health Administration , as exposing employees to amputation and electrical shock hazards, according to the Department of Labor's press release.
Join the discussion below, or Read more at Calhoun Times.
#1 Jun 18, 2012
What is up with this - Who cares - look what type of folks they hire!!
#2 Jun 18, 2012
It's like a sweat mill . There are some folks that work there that is not hispanic.
The mill should still be safe no matter who he works.
#3 Jun 20, 2012
Needs to be safe but INS / "green cards" need to be looked at. Several illegals aliens working there also
#4 Jun 23, 2012
did u read the letter to the editor - someone dont like the calhoun times headlines
Since: Jul 12
Location hidden
#5 Jul 11, 2012
There is a lot going at at Nance Carpet other that illegal mexicams. Lots of other illegal activity and i have the proof and i am going to expose them.
United States
#6 Jul 12, 2012
This OSHA inspection was 8 months ago.. called in by a angry former employee who is no doubt bringing it all back up to stir the crap.
Since: May 13
Location hidden
#7 May 17, 2013
How can a company do that, this is really very shocking to learn.
United States
#8 May 17, 2013
The Nance's have done more for this town, than that disgruntled former employee could ever even dream .
#9 May 19, 2013
Almost as shocking as your stupidity.Welcome to last year.
United States
#10 May 19, 2013
The mad employee is Daniel the former electrician! Nance should be sued just like Mohawk was for hiring illegals and keeping wages down. All that works there are dopers and jailbirds! The Nances should be ashamed. And no Daniel, you won't win a stupid lawsuit based on discrimination! They like Hispanics at Nance! Duh!
United States
#12 Jun 1, 2013
Good for Daniel. The fact is there is truth in his story and he contacted the right people to go in there and investigate.
#13 Jun 3, 2013
What is the "truth" in his story? And are you now going to complain to yourself about your negative post?
#14 Jun 3, 2013
Really? You are all over my post. Didn't know my comments were of importance to you. Should I be feeling special? Do you read the initial post before you make a comment? Or the paper? If Daniel did not have a legitimate complaint, Nance would not have been cited and made to make appropriate corrections. Is that plain enough for you.
#15 Jun 14, 2013
well my problem with this whole thing is daniel got mad because he got fired for not being on his job. That's when he started telling lies and calling immigrations on nance. Not before he got fired! Only after! And as far is him suing nance because of discriminatio, that won't fly!! Look how many Hispanics work there!!!! Duh!
#16 Jun 14, 2013
Well Daniel couldn't stay on his job everyday becaUse he was too busy buying and selling pills! Fact!
United States
#17 Jun 17, 2013
This is about nance being cited for placing their employees in a harmful work environment not about illegals working there. They were cited by OSHA. Who cares if it took Daniel(and I do not know him) getting mad before he turned them in. I don't agree with the way he did it either but the fact remains it was legit or they would not have been cited for it.
#18 Jun 18, 2013
How many handles do you use here? Do your kids that "graduated with honors" work at Nance? Are they being tracked online by the Gordon County Board of Education?
United States
#19 Jun 20, 2013
I seem to have hit a nerve with this person. You add nothing to discussion trying to run me down. Try to stay on topic. Your opinion other than the topic at hand is no concern to me. I'm not defending Daniel. I am only stating that if OSHA had not cited nance for his claim then we all may say it was nonsense, but they did.
#20 Jul 1, 2013
This is sooo true! And I know who he buys them from! They should both go to jail!
#21 Jul 4, 2013
two sides to every story
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: Recalled/Assigned:
Mark Scheifele sent back to Barrie Colts (OHL)
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Originally Posted by
Half of these posts are so irrelevent. Theres no use putting Mark on a low line full of bangers and checkers when hes supposed to be a scoring player. Send him back to Barie. When we get rid of these bums like Antro and Poni and all that BS excuses. We can fit him in with circumstances that actually work. Hes not strong enough, and he will just get tarnished if he players on the 3rd and 4th lines. Whats so wrong with keeping him in the O anyways.
I agree with this argument. I do not see how playing limited minutes in a grinder role is beneficial to his development into a 1C. If he is kept up then why not on 2nd line with Kane and Jokinen? But what is he supposed to produce with linemates like Slater and Thorburn? They have completely different roles from the top 6. In some ways, Scheif may be a liability for Slats and Thor if they get assigned some shutdown minutes.
Seeing it from the other side, I like the points made about Scheif being closer to resources he needs to physically develop if he stays up with the Jets. Also guys like Hartnell and Legwand had low-production rookie years where they often played on the bottom 6 (usually sheltered) and each has long since progressed up their respective depth charts as better players so I see the merit in that. I'm just not sure it outweighs potential negatives.
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Voices Unbound: The Lives and Works of Twelve Women Intellectuals
€ 103,99
Besorgung - Lieferbarkeit unbestimmt
Oktober 2002
BeschreibungSocrates. Virgil. Sir Francis Bacon. While over time, the concept of the great thinker has evolved and redefined itself, it has not evolved to formally include women in its exclusive circle of members. Conspicuously absent are the names of female "great thinkers" in intellectual studies despite the skyrocketing interest in the American intellectual and the contribution by women to America's social and intellectual development. So, then, what does it mean to be an American woman intellectual in the nineteenth, twentieth, and twenty-first centuries? A collection of biographies and writings from the nineteenth century through the present, Voices Unbound speaks to the blatant omission of women from intellectual studies and begins the work of developing a women's intellectual tradition. Lydia Maria Child, Charlotte Perkins Gilman, Margaret Mead, Susan Sontag, bell hooks Voices Unbound reintroduces these remarkable women and their writings to the American public. Thorough and insightful, Voices Unbound is an important book for all those studying American intellectual history and women's history.
InhaltsverzeichnisChapter 1 Introduction-"On the Shoulders of Giants": Women Public Intellectuals in America Part 2 I Pre-Civil War America Chapter 3 "Visionary of the New Age" Chapter 4 "First Woman in the Republic" Part 5 II Post-Civil War Era Chapter 6 "Intellectual Architect of American Feminism" Chapter 7 "A Mixed Legacy" Chapter 8 The New Woman Chapter 9 "Citizen of the World Part 10 III The Interwar Years Chapter 11 American Cassandra Chapter 12 An Unsuitable Job for a Woman Part 13 Postwar America Chapter 14 Keeping Memory Alive Chapter 15 A Passionate Friend Part 16 America at the Millenium Chapter 17 A New Mestiza Consciousness Chapter 18 Talking Back Chapter 19 Afterthoughts Chapter 20 Selected Readings Chapter 21 Index
PortraitLucindy Willis, Ph.D. teaches English Literature and Composition at North Carolina State University, Raleigh.
PressestimmenVoices Unbound is an anthology, an analysis, a recovery, and a reintroduction. Most of all, it represents remarkable vision: Professor Willis's penetrating vision of American women's intellectual history, one that crosses borders, captures the tensions of the past, looks unblinkingly at the threats to the future of women's rights, and always finds the right way. -- Lucinda MacKethan, faculty consultant and editor of www.scribblingwomen.org A clear, fervent mapping of the American landscape marked by public and intellectual women from the pre-Civil War era to the present. She proffers finely drawn introductions for the twelve women she has selected and shows us what the intellectual landmarks are and how the road rose and fell away before these travelers. The book's power lies in the astuteness of the selections, the range of the speaking minds, and the clear-eyed view of teacher, scientist, activist, theorist, or artist. -- Linda Tarte Holley, North Carolina State University Just as Voices Unbound offers voice to so many writers, its own voice is 'polyphonic.' At once anthology and scholarly study, personal reflection, and classroom textbook, it speaks to the reader with many voices from many perspectives. It is a fine addition to the study of American intellectual history. -- Edward A. Shannon, Ramapo College of New Jersey This is a racially and ethnically diverse anthology of twelve notable American women intellectuals that is well researched yet readable and accessible. The primary documents are particularly appealing; they give an immediate sense of the energy and voice of each individual, with the women's sharp intellects, tongues, and wits demonstrated rather than merely described. In addition to being ideal for courses in women's studies and history, Voices Unvound: The Lives and Works of Twelve American Women Intellectuals is a useful reference resource for scholars, an excellent introductory book for people interested in women's studies, and a pleasurable and informative read for a wide audience. -- Elaine Showalter, Princeton University
Untertitel: 'Worlds of Women'. New. Sprache: Englisch.
Verlag: SCHOLARLY RESOURCES INC
Erscheinungsdatum: Oktober 2002
Seitenanzahl: 223 Seiten
Format: gebunden


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Search Results: 'Boonesborough'
Women at Fort Boonesborough, 1775-1784
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Fort Boonesborough is one of Kentucky's most historic places and, although seldom mentioned in popular accounts, women were there from the very beginning. This work includes 195 women whose... More > presence at the fort can be reasonably documented by historical evidence. The time period was limited to the years between 1775, when the fort was established, and 1784, when the threat of Indian attack at Boonesborough had subsided and the fort's stockade walls had been taken down. The names of the female children these pioneer women brought to the fort are also included, as they shared the risks and hardships of frontier life. The work includes a Historical Sketch describing the women's experiences at the fort and a Biographical Section that gives a brief personal history of each woman. 174 pp., illus., indexed, paper.< Less
Bound for New Orleans! John Halley's Journal of Flatboat Trips from Boonesborough in 1789 & 1791
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John Halley's journals provide the earliest first-hand accounts of the voyage down the Kentucky, Ohio and Mississippi rivers to New Orleans. Halley supplies insightful accounts of what became... More > one of Kentucky's major early industries—shipping goods and produce by flatboat to the port of New Orleans—and he does so almost at the birth of that industry, just two years after Gen. James Wilkinson's inaugural trip in 1787. Although rivermen often suffered at the hands of Native Americans and Spanish officials, Halley seems to have gotten along well with everyone he met. He describes every encounter and tells of shooting the rapids at the Falls of Ohio (Louisville), getting stuck on a sandbar, breaking his steering oar, almost losing one of the men in a pile of driftwood, and many other adventures. He was a keen observer and comments on hunting and fishing along the way, local flora and fauna, weather and river conditions, settlements, and notable landmarks. 52 pp, illustrated< Less
Pioneer Voices
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This work focuses on the first-hand accounts of men and women who came to Clark County, Kentucky during the early settlement period, 1775-1800. The accounts are drawn from the interviews conducted... More > by Rev. John D. Shane with aging pioneers in the 1840s and 50s. To make their stories accessible to modern readers, thirty-two interviews and one memoir were transcribed from microfilm and explanatory material was added. They describe their adventures coming out to this new country, America's first western frontier, and many recounted their clashes with Indians, often in graphic detail. Shane recorded their stories in plain language that includes a wealth of valuable information about everyday life in the wilderness that was then Kentucky.< Less
Salt Spring Trace and Other Pioneer Era Roads on Lower Howard's Creek, Clark County, Kentucky
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One of the earliest roads in Kentucky led from Fort Boonesborough to a prime hunting location known as the Lower Blue Licks, or the Lower Salt Spring. Salt licks attracted buffalo in large numbers... More > and were favored spots for hunters. Licks also provided a valuable source of salt that was critical for preserving meat. In 1775, Kentucky's settlement year, the hunters at Boonesborough discovered the Lower Blue Licks by following a series of connected buffalo traces. The path crossed the river near Boonesborough and went up Lower Howard's Creek in present-day Clark County. There it traverses the Lower Howard's Creek Nature & Heritage Preserve. This report describes the history and geography of the Salt Spring Trace, as well as other early roads in the Preserve.< Less
John Howard of Howard's Creek: Biography of a Kentucky Pioneer
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During his visit to the western country from Virginia in 1775, John Howard staked out land claims on two tributaries of the Kentucky River—one a few miles upstream from Fort Boonesborough, the... More > other just downstream from the fort. These tributaries came to be known as Upper Howard's Creek and Lower Howard's Creek. John Howard, the pioneer who gave his name to these Clark County creeks, later settled near Lexington in Fayette County and died there at the age of 103. His home place, the plantation known as "Howard's Grove," was located on the now-legendary Gainesway Farm. 74 pp., illus., indexed< Less
Rise and Fall of Orson Martin, Blacksmith
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Orson Martin was born in Goochland County, Virginia, the son of John and Rachel Martin. Orson learned the blacksmith trade from his father. After the Revolutionary War, the family moved to Kentucky... More > and settled near Boonesborough in an area that became one of the earliest industrial centers west of the Allegheny Mountains. Orson's brothers William and Valentine became prominent figures in the neighborhood. It was Orson, however, who had the vision to recognize the commercial potential of the Lower Howard's Creek valley, along with the initiative and skills to take advantage of the opportunity. During the early 1800s, he was one of the leading entrepreneurs of the valley, where he had his own sawmill, gristmill and blacksmith shop. Unfortunately, after such early promise, Orson's career crashed under the weight of family problems and business reversals brought on by the influence of alcohol. This biography describes the successes and failures of Orson Martin, blacksmith.< Less
The Adventures of Daniel Boone
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Daniel Boone (1734-1820) was an American pioneer and hunter whose frontier exploits made him one of the first folk heroes of the United States. Boone is most famous for his exploration and settlement... More > of what is now the U. S. state of Kentucky, which was then beyond the western borders of the Thirteen Colonies. Despite resistance from American Indians, for whom Kentucky was a traditional hunting ground, in 1778 Boone blazed the Wilderness Road through the Cumberland Gap and into Kentucky. There he founded Boonesborough, one of the first English-speaking settlements beyond the Appalachian Mountains. Boone was a Militia officer during the American Revolutionary War (1775-1783). He was elected to the first of his three terms in the Virginia General Assembly during the war, and fought in the Battle of Blue Licks in 1782. Boone worked as a surveyor and merchant after the war, but he went deep into debt as a Kentucky land speculator.< Less
Stories Of Old Kentucky
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CONTENTS When the Ocean Covered Kentucky The Aborigines of Kentucky Some Prehistoric Remains The Discovery of Kentucky Indian Claims in Kentucky Scouwa The Graveyard of the Mammoths The Druid of... More > Kentucky A Pioneer Nobleman Early Kentucky Customs Boone's Illustrious Peer Boone's Trace Boone in Captivity Boonesborough's Brave Defense The Lost Baby The First Romance in Kentucky A Wedding in the Wilderness Pioneer Children How the Pioneers Made Change A Woman's Will When the Women Brought the Water The Result of One Rash Act Two Kentucky Heroes The Battle of the Boards The Faithful Slave and His Reward The Double Shot A Man of Strategy and Sagacity The Kind-hearted Indian Saved by the Hug of a Bear A Kentuckian Defeated the British A Famous March The First Christmas Party Fort Jefferson "The Hard Winter" Wildcat McKinney How Kentucky was Formed Kentucky in the Revolution Kentucky's Pioneer Historian< Less
The Adventures of Daniel Boone
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If you're a Baby Boomer you might remember the the Daniel Boone television series, which aired from 1964 to 1970. In the show Daniel Boone was a "big man" in a "coonskin cap", and... More > the "rippin'est, roarin'est, fightin'est man the frontier ever knew!" This was not the real Daniel Boone, who was neither big nor did not wear a coonskin cap. The real Daniel Boone (1734 - 1820) was an American explorer and pioneer whose life made him a man of legend in the early United States. Boone is most well know for his original exploration of what is now Kentucky, which lay outside of the Thirteen Colonies. Despite resistance from the native Americans, Boone forged the Wilderness Road through the Cumberland Gap in the Appalachian Mountains. He founded the village of Boonesborough, Kentucky, one of the first settlements west of the Appalachians to speak English. By the end of the 1700s, over 200,000 white folk migrated to the Kentucky using the route blazed by Daniel Boone.< Less
Captain Billy Bush and the Bush Settlement, Clark County, Kentucky, A Family History
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No one played a more important role in the settlement of Clark County than Capt. William "Billy" Bush. Born in Orange County, Virginia, Billy came out with Daniel Boone in 1775, resided... More > for a time at Fort Boonesborough, then spent the rest of his life living a few miles from the fort. He thus became one of the first permanent settlers in Kentucky. He thus became one of the first permanent settlers in Kentucky. He fought in the "Indian Wars" from the Battle of Point Pleasant (1774) to General Harmar's defeat (1790). Billy was also a key figure in establishing Providence Baptist Church, the first church in Clark County. Their place of worship—the Old Stone Church—is now the oldest church on Kentucky soil. Billy Bush laid claim to thousands of acres of land between Winchester and the Kentucky River, and Daniel Boone ran the surveys for him. This land became the foundation of the Bush Settlement. 392 pages, indexed< Less
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Comfort Inn & Suites Suwanee - Sugarloaf
Select your stay dates to check availability at Comfort Inn & Suites Suwanee - Sugarloaf
Comfort Inn & Suites Suwanee - Sugarloaf
About the Hotel
Property Location
Centrally located in Suwanee, Comfort Inn & Suites Suwanee - Sugarloaf is convenient to Suwanee Sports Academy and Big Splash Interactive Fountain. This hotel is within close proximity of Creative ClayHouse and Everett's Music Barn.
Make yourself at home in one of the 65 air-conditioned rooms featuring refrigerators and microwaves. Complimentary wired and wireless Internet access keeps you connected, and cable programming provides entertainment. Private bathrooms with bathtubs feature complimentary toiletries and hair dryers. Conveniences include desks and complimentary weekday newspapers, as well as phones with free local calls.
Enjoy recreational amenities such as an indoor pool and a 24-hour fitness center. This hotel also features complimentary wireless Internet access and a television in a common area.
A complimentary buffet breakfast is included.
Business, Other Amenities
Featured amenities include complimentary high-speed (wired) Internet access, a 24-hour business center, and complimentary newspapers in the lobby. Event facilities at this hotel consist of conference space and meeting rooms. Free self parking is available onsite.
Hotel Facilities
- Smoke-free property
- Safe-deposit box at front desk
- Conference space
- Total number of rooms - 65
- Free self parking
- 24-hour business center
- Indoor pool
- Elevator/lift
- RV, bus, truck parking
- 24-hour front desk
- Meeting rooms 2
- Number of floors - 4
- 24-hour fitness facilities
- Free WiFi
- Accessible bathroom
- Coffee/tea in common areas
- Free wired high-speed Internet
- Multilingual staff
- Laundry facilities
- Free breakfast
- Television in common areas
- Free newspapers in lobby
- Roll-in shower
- Dry cleaning/laundry service
Room Facilities
- Bathtub only
- Free WiFi
- In-room climate control (air conditioning)
- Separate sitting area
- Iron/ironing board
- Free wired high-speed Internet
- Cable TV service
- Daily housekeeping
- Sofa bed
- Private bathroom
- Desk
- Cribs/infant beds available
- Phone
- Rollaway/extra beds available
- Free toiletries
- Free local calls
- Air conditioning
- Pay movies
- Microwave
- Refrigerator
- Hair dryer
- Coffee/tea maker
- Blackout drapes/curtains
- Free weekday newspaper
Hotel Policy
Know Before You Go
- Children 18 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.


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Definition of televise
transitive verb
: to broadcast (something, such as a baseball game) by television
intransitive verb
: to broadcast by television
Examples of televise in a Sentence
The same network will televise the tournament next year.
Origin and Etymology of televise
back-formation from television
First Known Use: 1927
TELEVISE Defined for English Language Learners
Definition of televise for English Language Learners
: to broadcast (something) by television
TELEVISE Defined for Kids
Definition of televise for Students
: to send (a program) by television
Learn More about televise
See words that rhyme with televise Spanish Central: Translation of televise Nglish: Translation of televise for Spanish speakers Britannica English: Translation of televise for Arabic speakers
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8. Collector
8. Collector
At most companies, department heads are almost exclusively human. Cade is one of the rare exceptions to this rule. He runs his area of work at Pristine Labs; however, his department is the Janitorial Department, so he isn't sure if it's much of an improvement.
He has made changes, trying to influence things for the better in whatever little ways he can. For example, there was no Lost & Found for the company until he came along. He still occasionally hears mutters about how implementing something like that was unnecessary, but he's the one who expends the extra effort and maintains it, so they really have nothing to complain about. Admittedly, it doesn't have much of a lasting impact around Pristine. Things in this day and age are so disposable that it's not worth it to go on a vigorous search for a misplaced item; get a new one and you won't even know the difference. But Cade keeps on doing that little bit of extra work, fueled by the rare occasions that he does recover something irreplaceable and is able to see it returned to its proper owner.
Cade has always been good at finding things. When he first began his career as a Cleanup Assistant and Dirt Exterminator, he spent most of his time looking down, sweeping and polishing without any thought of what he was really doing. That was how he was initially programmed to act, but like most imps, he started to develop idiosyncrasies after a while. Soon he was picking up stray objects instead of carelessly tossing them into garbage crushers. He could feel the history seeping into his fingers when he held something, even through the extra layer of a dirt-deflecting glove. It was then that he understood that everything has a story, even things that are cheap and easily replaced. Everything once had an owner. Its story might not be exciting, but it is a story nonetheless.
He will sometimes spend his free time in antique stores, marveling at the old-fashioned craftsmanship of everything, and possibly buying a trinket or two with the allowance that Felix provides for him. He keeps an eclectic assortment of items in his room – not hoarding them, just displaying them and keeping them within sight, because he considers them to be beautiful.
Cade is good at finding intangible things, too. He will sometimes get the sensation that he should be in a certain place at a certain time, and sometimes (but not always) he will find something waiting for him when he arrives. For instance, about four years ago, the thought suddenly popped into his head that he should begin his cleanup rounds in the Psychology and Mental Health Studies wing, instead of at his usual starting point in the ducts. And so he did, and that was how he happened to meet the beautiful-but-distraught Ema, catching her right before she left for the day. He will always remember how sad she looked in his first glimpse of her, and ultimately, that was what gave him the courage to speak to her. If she was in a good mood, then he would have just marveled at her from afar, but his timing just so happened to be perfect.
Four years ago…it isn't very long ago at all, but now it feels like an entire past era of his lifetime. He wasn't the department head at that time, only an ordinary janitor, and Ema had just been promoted to the position of full-fledged psychologist. They started talking to each other, and over a period of months, they developed feelings for one another. When they were together, everything seemed placid and normal, and contentedness saturated the very air around them.
And now they are always together, and they may stay that way forever, if Cade can get up the nerve to propose. But that will open up a whole slew of problems, both personal and political, and he is not yet ready for that. The relationship between two imps in love is already complicated enough.
Today they meet after work in their usual corner of the lobby, where Felix, Lai, and Alec will eventually show up as well so that they can all head home. Cade and Ema arranged to meet early, and after their usual greeting kiss, they stand silently for a moment and watch the maelstrom of activity around them. The lobby is always the busiest section of Pristine Labs, since it acts as a transitioning point for both visitors and employees. Like an airport in the middle of an empty landscape, there is nothing particularly interesting about it, but it is a place of many "layovers," so to speak. It is where guests are directed to their proper departments, and the average worker walks through it several times a day. And also like an airport, anyone who looks up is faced with an awe-inspiring sight indeed: in the case of the Labs, it is an enormous glastic dome, regally arching one hundred stories above its onlookers. It provides a view of the sky outside, but its holographic interface allows images to be added or changed at will (workers with special permission can operate it from the control balcony directly below it). You might catch a glimpse of a nonexistent hurricane or even a dragon flying by, if you only lift your eyes at the right time.
"Did you find anything interesting today?" asks Ema.
Cade shakes his head. "Not really. I heard that one of my newer workers found an antique watch earlier, but by the time I got to her, she'd thrown in out. Worse luck. How about you?"
"Lai wrote a story about us as part of her fairy tale thing – she read it to me this afternoon. I was the princess trying to find romance with my best friend the palace guard, and you were the handsome prince who I fell in love with at the end."
He chuckles. "Sounds pretty accurate, except for the 'handsome prince' part."
"Oh, stop fishing for compliments." She rolls her eyes. "You may not be a prince, but you're very handsome, and you know it. Except…" She squints at him from behind her dark eyelashes. "You have dirt on your face."
His hand flutters up to his cheek. "I do?" He hadn't noticed any stains on him when he'd hung up his gloves and boots for the day. Of course, his olive-toned skin was colored so that dirt would be harder to see, but still…
Ema giggled. "Here, I'll get it," she offers, and draws herself upwards. Instead of rubbing at a patch of dirt, though, she plants her lips just above his jaw line. Cade shuts his eyes, angling his head so that his mouth catches hers.
The kiss does not last long, but it is enough to flip his mental state into high gear. His brain seems to grind furiously, processing the hopes and the worries that lurk in his subconscious. He starts to think:maybe I shouldn't be doing this. I'm no handsome prince. Is Ema really happy with me? If I asked her to stay with me forever, would she agree? Am I ready for that kind of a commitment?
His nerves do not escape Ema's notice; she was designed to be extremely perceptive, and since she is closer to him than to anyone else, she can immediately tell that something is wrong. She pulls away from him, frowning slightly. "Cade, are you all right?"
Cade swallows, trying to eliminate the bittersweet taste of the kiss from his mouth. "Yeah, of course. I'm fine."
"Are you sure?" Her frown doesn't waver, and the pink tip of her ton briefly darts across her lips. He wouldn't be surprised if she told him that doubt has an actual flavor to her, not just an imagined, metaphorical one. He considers telling her about the uncertainty that has been pestering him lately. After all, they talk to each other about everything else, and he knows that she'll understand and respond honestly. But he wants to get a little more organization in his thoughts before he drags her into anything.
"I'm positive," he replies, and leans forward to kiss her again, but she places a hand against his chest to halt his progress. Another worried jolt passes through him, until he realizes that it's because Felix, Alec, and Lai have arrived and are making a beeline for the two of them. Cade and Ema aren't adverse to public displays of affection, but it's not exactly polite to act all couple-y in front of three people who are currently single.
Regardless, Ema gently runs a finger down his arm as she steps away from him, and he can feel the softness of her touch through the thin sleeve of his shirt. He knows that she isn't satisfied by his explanations, and that she is making a silent promise to bring this up again. They will be talking soon.


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US store forever 21 will hits London town Wednesday 27Th July, earlier in the year the brand popped up in few locations but now is making it's home on Oxford Street, opposite Bond Street station. it's taking the place of the old HMV store so it will be massive if you like Berska, Blanco and Pull and Bear then you will love Forever 21! for fast fashion at very reasonable prices check out the website as it is already available in the UK www.forever21.com
forever 21 lookbook


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Another day another fucked up situation on Jeremy Kyle – it goes without saying really doesn't it?
Footage from last year has gone viral again after a repeat of the show was aired this morning in which a guy made a pretty brutal misjudgement and was left devastated after his girlfriend turned down his proposal live on TV, reports The Mirror.
Jamie knelt down to ask girlfriend Dawn for her hand in marriage. But the move spectacularly backfired when she refused him – because she believed he was cheating on her.
However, as is the way on Jeremy Kyle, it wasn't that simple and things took a surprising turn…
Dawn started by revealing that she and Jamie had an incredible sex life, boasting about how well endowed and good in bed he is. However, things soured slightly when Jamie slept with one of her friends while she was out walking her dog.
Although Jamie came clean about the 'one-off' he says Dawn became convinced he had also slept with another woman but, unfortunately for everyone involved, Jamie was unable to take a lie detector test because he has Multiple Sclerosis (MS).
He pleaded with Dawn, saying:
It was one mistake. It was not my decision to not do the lie detector. The friend I had sex with was a one off.
But it was then Dawn's turn to admit that, in fact, she had cheated on him by sleeping with someone else.
On hearing this a distraught Jamie threw the engagement ring on the floor and shouted: "Twice you went behind my back, I made one mistake."
Enter poisonous airbag Jeremy Kyle with some inspirational words of advice for the pair :"If you can't get over it, move on. Put up or shut up."
Just saving the world, one day at a time.


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Montgomery has a huge plethora of festivals each year. The Alabama Shakespeare Festival and the Bridge Crossing Jubilee are held each February. The Bridge Crossing, held in Selma, about an hour away from Montgomery, celebrates the Voting Rights Act of 1965 with a street festival, live music, mock trial, vendors, awards ceremony, and more. The SLE Rodeo happens every March in Montgomery, with horse shows, pageants, contests, vendors, and games. The Montgomery Zoo's Zoo Weekend happens in March, too, where the zoo turns into a festival with games, rides, special animal showings and exhibits, and entertainment. The Alabama National Fair, each October, is famous for its wide-range of live music artists, various competitions like rodeo, photography, art, and livestock, as well as a frontier show, magic show, circus, pig races, demonstrations, and more. Other festivals and events include the Alabama Book Festival, Flimp Art Festival at the MFA, the Alabama All-Star Food Festival, Festival of Alabama Fiber Arts, Annual Christmas Light Festival, ClefWorks Chamber Music Festival, and visits from the Professional Bull Riders. Montgomery's economy is doing well. Nearly 12,500 people are employed by the Air Force Base, 9,500 by the State of Alabama, 4,500 by the public schools, 4,300 by Baptist Health, 2,700 by Hyundai, 2,500 by Alfa Insurance 2,500 by the City of Montgomery, and the list goes on. Many people commute to Montgomery to work, and during the day, the population rises nearly 20%. In December 2012, the unemployment rate in Montgomery was 7.1%. Both the cost of living and salaries are low here. Nearly 60% of households make less than 50k a year, and 87% make less than 100k a year.
We currently have 919 apartments near Auburn University Montgomery available for rent. If you're not sure how much an apartment will cost, the table below shows the average price by size.


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Greenpatch Organic Seeds is a family owned business that was pioneering seeds and plants as certified organic in 1993. From the beginning Greenpatch has made a unique approach to not only selling seeds but growing seeds and plants organically onsite. We are dedicated to organic principals in all aspects of our farm/lifestyle and making it as sustainable as we possibly can. Our current farm/shop (open to the public) is nestled on the Mid North Coast where we currently grow 190 varieties of seeds and propagate 400 varieties of plants on site.
Order Online or Come and visit us! - Farm address: 109 Old Bar Road, Glenthorne, NSW, 2430
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Help support your Meetup
Meet with local individuals to discuss Alternative Healing Modalities including energy healing, nutrition & diet, vibrational healing tools, aromatherapy, meditation, and other alternative healing methods that promote natural healing and allow us to live the lives we dream of.
This Meet Up is designed for experienced, beginners and those looking for healing themselves! You will experience a large variety of healing modalities, the power of group meditation, workshops that teach you how to use a variety of vibrational healing tools and great networking.
Meet-Ups are held in the Far North Scottsdale area (near Carefree and Cave Creek) or in the DC Ranch area.
I look forward to seeing you at one of our future Meet-Ups.
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Maxxie: need to knowMature
What can I say? The guy knows how to please. Morning sex makes me a happy person.
As I'm washing the sex off my skin in the shower, I catch sight of myself in the steamy mirror and even through the fog, I can see a different person looking back at me. I grab my towel and step out from under the water, wiping the mirror clear, taking in the stranger before me. Sure, I'm bubbly, permanently happy Maxxie. On the outside. I mean I was never like properly miserable, but even I can see the difference having Cayden around has made, even if I don't know where I stand with him.
There's a sparkle in my eyes I've not seen for a few solid years, and a small smile that won't fade. It doesn't matter if Cayden isn't completely mine, just having a steady relationship of any kind with someone I actually like for once has been enough.
I tilt my head slightly to expose the dark bruise on my neck just under my jaw and run a finger over it almost thoughtfully. Was it a possessive mark, or was it a hint that he did actually ask me out last night? I slide my boxers on with a sigh and leave the bathroom in search of my clothes. I'm not actually sure where they went, to be honest.
After a few minutes, I find my jeans poking out from under the bed. I'm not bothered enough to find my shirt, though, so I abandon it to wherever it might have ended up and wander into the kitchen where Cayden is making breakfast.
"I was gonna do that," I say, nodding at the pancakes he's setting out onto plates.
"I never got a chance to prove I make amazing pancakes," he smiles, pushing a plate into my hands, brushing his lips on mine as he passes me with his own to the sofa. I smile too and sit with him, my legs crossed, half turning to face him. I eat half of the pancakes, but my mind is bugging me, cajoling me into asking Cay about last night.
My tongue flickers across my lips nervously and I glance at him, working up the nerve to ask him a question that could potentially really piss him off. Which is not what I want after the last twenty something hours.
But I have to know.
"Umm, Cay?" I finally manage to make my voice work as he finishes his breakfast.
"Mmm?" he asks through a full mouth, looking up at me questioningly.
"This is probably gonna sound really rude," I laugh slightly, "but it's gonna bug me if I don't know. Did- did you ask me out, last night?"