text:
PROVO, Utah (AP) - JJ Di Luigi rushed for 134 yards and a touchdown and Bryan Kariya ran for another 85 yards and two touchdowns as BYU avoided a 40-year low with a 24-21 win over San Diego State.
Di Luigi finished a bruising 19-play opening drive with a three-yard touchdown run as the Cougars (2-4, 1-1 Mountain West) snapped a four-game losing streak. The last time BYU lost five in a row in a single season was 1970.
Ryan Lindley threw for 220 yards and three touchdowns for San Diego State (3-2, 0-1), which came into the game ranked 24th in the nation in rushing.
BYU beat the Aztecs at their own game, holding San Diego State to minus-2 yards rushing in the first half and 53 for the game.
Kariya scored on a two-yard run late in the first quarter and a one-yard burst early in the fourth quarter to give BYU a 24-14 lead.


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Indian Ordnance Factory Recruitment 2016 - 17 Jr Works Manager 188 Posts
Indian Ordnance Factory Recruitment 2016
Ordnance factory has Released Recruitment Notification for 188 Junior Works Manager.This organization has invites applications to filling up the 188 vacant position. Great news for all candidates who have looking for latest government jobs. So candidates grab this excellent opportunity and make your career with this organization.
Eligible and Interested candidates who are willing to do the job and meets the eligibility criteria can apply through online mode from its official website on or before the last date of application submission.
Further Detailed Information related to Indian Ordnance Factory Recruitment 2016 such as Educational Qualifications, Age Limit, Mode of Selection, application fee, important dates, how to apply, salary offered is mentioned below of this page, in this post or on the notification that available on the official website.
Descriptive Information:
· Organization Name: Indian Ordnance Factory
· Number of Posts: 188
· Official website: http://ofbindia.gov.in/
· Job category: Government
· Mode of Apply: Online
Vacancy Details:
· Junior Works Manager(Civil)- 04
· Junior Works Manager(Clothing Technology)- 09
· Junior Works Manager(Electrical)- 05
· Junior Works Manager(Leather Technology)- 02
· Junior Works Manager(Chemical)- 06
· Junior Works Manager(Mechanical)- 153
· Junior Works Manager(Metallurgical)- 09
Eligibility Criteria:
Age Limit: Kindly refer to the official advt.. Age relaxation for reserved community candidates will be given as per the rules and regulations of organization.
Read Also: Calculate your Age for Government Job
Educational Qualification: Graduation & B.Tech/ B.E from a recognized board and university
Selection Procedure:
- Written examination
- Personal interview
How to Apply:
- Firstly candidates need to visit the official website which is www.upsconline.nic.in
- Search the appropriate link of the recruitment
- Read full Notification carefully.
- Download application form
- Now, fill the application form with required details.
- Attach scanned photo, signature and documents.
- Submit the application from
- At last, take printout of it for future use.
Read Also: Learn to Fill up Online Application Form
Important Dates: Last Date for Application Submission: 28th July 2016
Note: For more information candidates may visit the Official Website.

subset: commoncrawl


text:
Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I just received a jury summons letter requesting me to do jury duty for March 18th. My current job will end March 7th.
During that time, it is very important that I search for jobs, and I need to be available for interviews of course.
Can I be excused from jury duty if I send them a letter from my employer explaining all of this, and I write a cover letter explaining that I need that time to look for a job and interview. The timing is just really bad.
Thank you
Financial hardship is sometimes an excuse to get out of jury duty, but I'm guessing that NO ONE thinks any time is good to perform this civic duty, and I know that many, many people claim this hardship and still have to show extenuating circumstances. Courts are too busy and need too many jurors to just use those who are independently wealthy or retired or whose employers pay for them while they're on jury duty.
Disclaimer: Please note that this answer does not constitute legal advice, and should not be relied on, since each state has different laws, each situation is fact specific, and it is impossible to evaluate a legal problem without a comprehensive consultation and review of all the facts and documents at issue. This answer does not create an attorney-client relationship.Ask a similar question
A jury summons can be postponed. Call the jury commissioner or go online and request to move jury duty. However, I think your logic is wrong. Jury duty is best now rather than after you start a new job. You even get some money for serving. I do not think a judge will excuse you from serving because you may have an interview.
The response given is not intended to create, nor does it create an ongoing duty to respond to questions. The response does not form an attorney-client relationship, nor is it intended to be anything other than the educated opinion of the author. It should not be relied upon as legal advice. The response given is based upon the limited facts provided by the person asking the question. To the extent additional or different facts exist, the response might possibly change. Attorney is licensed to practice law only in the State of California. Responses are based solely on California law unless stated otherwise.Ask a similar question
No, being unemployed is probably not sufficient to be excused from jury duty. I often have unemployed persons serving as jurors in my jury trials.
However, if the timing is bad, you have the right to seek a postponement of your jury service.Ask a similar question
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text:
Built by Access Manufacturing, Inc. Since 1972
A family owned and operated company, Access Manufacturing Inc. has been the forefront of the Aluminum Truck Top industry since 1972. With a dedication to quality and customer service Access Manufacturing custom builds truck tops to meet the meeds of any trade. We currently sell to private dealers and to the general public at our facility store in Winters, California.
Wanted to drop you a quick note to mention that we are very much enjoying the trailer. Pretty much everyone we know wants a
My tradesman shell was put on my new 1996 Chevrolet truck. Since then it has seen 100,000 miles of freeway, 5,000 miles


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Oscar Wesoul has no description.
·Oscar Wesoul·: 19 YEARS OLD! My style; Deep house ,tropical house ...Music is my passion and I want to show it and spread it around the world!! Thank You Everybody!!
'' ¿My secret? Love what I do and do what I love ''
Enjoy my weekly sessions and some of my productions, deep house and tropical music for youo to chill your minds up!


text:
I am in full support of health care for all Americans.
Not just the health care to fix what is wrong at that this time but to make sure that all Americans are becoming healthier.
When you look at statistics you find that it is our community where the people who are getting sicker, aging worse and also dying at a severely higher rate than like situated communities. We are living in a country that spends high amount of money on health care and it is time that the ones of us who need it the most have the same access as others.
Again, we must take responsibility. Prevention is a very high priority in health awareness. We need to insure that all our neighborhoods have access to hospitals or facilities that are capable and staffed to handle the issues of the neighborhood. We need more and better doctors.
I believe that with all of this taken into consideration as your representative and your voice in the legislature that it is my duty to advocate and provide a stable and more sustainable health care for the people.
Source: http://annazettecollins.com/issues/


text:
Britain's May to meet Goldman, Amazon, IBM to discuss post-Brexit investment
NEW YORK, Sept 19 (Reuters) - British Prime Minister Theresa May will meet business chiefs from firms including Goldman Sachs , IBM and Amazon later on Monday in an attempt to reassure investors after Britain's shock vote to leave the European Union.
May wants to encourage trade and investment between the United States and Britain, a spokeswoman for May said.
Also at the meeting in New York will be chiefs from AECOM , Morgan Stanley, BlackRock, Merck & Co, Sony Pictures and United Technologies Corp , the spokeswoman said.
Representatives from Thomson Reuters, the parent of Reuters, will also attend the meeting, the spokeswoman said. (Reporting by Guy Faulconbridge; Editing by Chizu Nomiyama)
© Thomson Reuters 2017 All rights reserved.


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This beautiful case is specially designed for Samsung Note 4. Made up of metal (aluminum), it can protect your phone from daily bumps or scratches. The four arc circle corners provides buffer function to prevent bumps and scratches. The metallic color adds more elegance and nobleness to the high-end Galaxy Note 4. The exquisite workmanship make all the holes accurate for all controls and buttons.
-Simple and elegant design with metallic electroplated alumimum is the perfect match for your new Samsung phone
-Ultra durable and light metal frame not only protects edges, but also prevents back and fronts from scratches
-Cover made with 100% aluminum
-Compatible with Galaxy Note 4, full access to all controls and buttons
-Shinning metallic frame makes your phone stand out from others
-Available in 4 colors
|Color of product
|Black, Gold, Red, Silver
|Case Functions
|Bumper, One Piece Case
|Device Compatibility
|Galaxy Note 4
|Gender or Style
|For Boys, For Girls, For Men, For Women
Customer Reviews
- Excellent product Review by Sammy
Price Quality Value
- Excellent product Review by Sammy
Price Quality Value
Write Your Own Review
Refund/ Return Policy
Any product purchased through us can be returned for a full refund or exchange within 30 days from the date you made your order. Please read the below steps explaining how to exercise this.
1. Request a return number by e-mailing us or using on our website contact form before returning the product. Please include your name, order number, the reason for the return, and if you want to return for a full refund or exchange for another product. Within 1 to 2 business days after submitting the request you will be contacted via email with an RMA number and product return instructions.
2. Returned items must be complete condition and not missing necessary parts. When returning a product, please write your RMA number on the package.
3. We will not pay the return shipping costs for non-defective returns. It is also the customer's responsibility to ensure that the returned goods are packed properly.
4. Refunds will be issued back to the original payment method. The refund will be issued the same day as we receive your returned product. If the product is not required to be returned, the refund will be issued on the same date as we specify to you by email.
If you wish to get a replacement of the same product because of a defective product after 30 days from the date you made the order, all products we sell come with our own 6 month replacement warranty from the order date. Please email us with your order number and description of the defect for a replacement. We can send the same product or an equivalent product of similar value that you specify.


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InMyBar - Drink & Cocktail Recipes
By Quintal Technologies, Inc.
Open iTunes to buy and download apps.
InMyBar is the best drink & cocktail app available because it can tell you what you can make with what you have.
InMyBar is a Drink and Cocktail app for iPhone, iPod touch and iPad users who want to know what drinks they can make with the ingredients they have and what they need to make their favorite and popular drinks. InMyBar is designed around a feature called My Bar with three main categories: What Do I Have, What Can I Make and What Do I Need.
Similar to some other cocktail apps, you can use InMyBar to look up drink recipes for almost any drink out there. But why just have a drink recipe catalog like so many others when you also can have a great a way to find just the drinks you can make with what you have? That's right, unlike other bar apps, InMyBar allows you to select the actual brand names of ingredients that you have and can display only the drinks that you can make based on those specific ingredients. Select the spirits, mixers, fruits, juices, etc. and InMyBar will tell you exactly what drinks you can make with detailed instructions on how to make them. Every ingredient and every drink has a picture of the actual item and you can click on any picture to see a full size version of it. Almost all drink recipes can be made without any specific brand name ingredients so even though InMyBar has brand detail selection, the drink recipes seldom require a specific brand. The What Do I Need view tells you what you are missing in order to make your favorite and our top rated drinks. It even lets you add items to a shopping cart so you have a list of what to buy.
InMyBar offers you the ability to add your own ingredients and your own drink recipes to our list of more than 1,350. Create a drink with the ingredients and measurements exactly the way you like to make it and even add a photo of the drink. You can also clone a drink recipe so you can copy, rename and edit your own version.
Ingredient Specific Features:
* 2,154 ingredients to choose from including spirits, mixers, juices and others
* Includes pictures of every ingredient (the bottle, can, fruit, etc.) to visually help you find the right one
* Browse or search all ingredients
* Search an ingredient for drinks that contain them to find out what you can make with some of your less common spirits
* Delete an ingredient if it is something you would never use
* Add your own ingredients if you find something we missed
* Add an ingredient to your shopping cart
Drink Recipe Features:
* 1,334 carefully selected, manually edited and spell checked drink recipes
* Includes pictures of what every finished drink should look like
* Recipes for virtually all of the most popular mixed drinks
* Includes every drink from the International Bartenders Association (IBA) Official List
* See what you need to make the Top 50 drinks
* Recipes for 657 cocktails, 191 martinis, 141 shots, 140 themed drinks and hundreds more
* Browse or search all drink recipes
* Delete drink recipes that you don't want to see or make
* Add your own recipes to make a drink just the way you like it
* Flag drink recipes as favorites to make sure you have the ingredients for them
* Add all ingredients needed for a drink to your shopping cart
Extra Features:
* MyBar feature tells you exactly what drinks you can make and what you need
* Shopping List feature
* Facebook, Twitter and Email features
* Pick or Shake for a Random Drink
* No advertisements taking up valuable space on your screen
* Bar Profiles to track the inventory for multiple bars such as your home, a friend's house, etc.
* No Internet connection required
* Includes an FAQ section for reference
* Free future upgrades with more ingredients, drinks and new features
* Full app help, which is especially useful for learning how to add your own ingredients and recipes
* Web site AND EMAIL support for questions, comments, suggestions and to report any problems (email@example.com)
IMPRESS YOUR FRIENDS with your new bartending skills
What's New in Version 1.3.3
Version 1.3.3 has import bug fixes.
★ We had an ongoing bug that is FINALLY resolved with this release. If a Bar Profile was added with a single or double quote in it (e.g. Dave's Bar) the app would crash the next time you started it. This had to do with the quote causing a problem with the database look ups. A reinstall from the App Store (free) fixed the problem before but you lost your customization. If anyone is having that crashing problem now and hasn't reinstalled the previous version, this update will allow you to access the app and all your data again. You will need to rename the bar profile causing the issue before setting it to your active bar profile by going to 'More -> Manage Bar Profiles'. All ingredients in the bar profile will remain intact.
★ Updated the sharekit framework to address several issues.
★ Please email us at email@example.com if you have any issues. The input we had from a customer with the quote problem helped us solve it for everyone. Thanks Dave!
Version 1.3.2 has tons of new drinks and ingredients.
★ Just in time for the 2012 professional football season, InMyBar now has over 70 football themed drink recipes. Pick your favorite team and try out one of the fun drink recipes.
★ Added 38 other new drink recipes including the Bahama Surprise, Black Cosmo, Headless Horseman, Left Handed Screwdriver, Liquid Carmel and the Sex Machine.
★ Added all ingredients requested by our customers on our InMyBar.com web site including Absolut Boston, E'Dolce Amaretto, DeKuyer Apple Barrel Schnapps, Jose Cuervo Classico Tequila, Stirrings Simple Syrup, Cherry Coke, Moxie, Three Olives Vanilla Vodka and the Cruzan Rum types Aged Dark Rum, 151 Rum, Aged Light Rum, Citrus Rum and 9 Spiced Rum.
★ Added a total of 200 new ingredients including 10 brands of Bourbon, 22 brands of Brandy, 3 brands of Coffee Liqueur, 6 brands of Gin, 10 brands of Rum, 22 brands of Scotch, 24 brands of Tequila and 32 brands of Vodka.
★ Some of the new brands are 1800 Coconut Tequila, 99 Whipped Cream Schnapps, Bacardi Black Razz Rum, Bacardi Black Rum, Bacardi Wolf Berry Rum, Coconut Jack Coconut Rum, Indiana Vodka, Jim Beam Red Stag Bourbon Spiced, Malibu Red Rum, Mount Gay Black Rum, Patron XO Dark Cocoa Liqueur, Pinnacle Cookie Dough Vodka, Seagrams Citrus Rum, Seagram's Raspberry Twisted Gin, Skyy Infusions Coconut, Skyy Infusions Raspberry, Smirnoff Coconut Vodka, Southern Comfort Cerry, Stoli Hot Vodka, Three Olives Vanilla Vodka, Vincent Van Gogh PB & J Vodka and Zephyr Blu Gin.
iPhone Screenshots
Customer Reviews
Awesome bar app
I love this app! We just finished a rather large residential bar. Not being a professional bar tender, I wasn't sure what I needed to stock the bar or how to make many drinks I don't normally make. This app tells you what you need, gives you a shopping list so you won't forget when you ge to ABC store, and gives you so many recipes and instructions. It's never crashed or given me any trouble. Highly recommend it!!
Best of the bunch
But the bar is low for these cocktail apps (I tried out several of them). This apps fixation on name brand spirits and mixers is annoying. There are many hoops to jump through (and I don't always get it right) to simply add generic ingredients to "my bar". I do like that you can clone a drink. Also like that you can select at least one ingredient and search for drinks with that ingredient (other apps allow you to choose more than one ingredient). Shopping list can be hard to find. I do love my cocktails so I use this app frequently (hic).
THE Best Bartender App
I cannot say enough good things about this app. You can go through your fridge and cabinets, and find and make drinks you wouldn't have even thought of. You can search drinks by individual ingredients, make shopping lists, see what the drinks look like, know how to make em, and impress your friends. It's amazing!
Customers Also Bought
- $0.99
- Category: Food & Drink
- Updated: Dec 07, 2012
- Version: 1.3.3
- Size: 23.3 MB
- Language: English
- Seller: Quintal Technologies, Inc.
- © 2012 Quintal Technologies, Inc
Compatibility: Requires iOS 4.3 or later. Compatible with iPhone, iPad, and iPod touch.


text:
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LONDON (AFP) – A duck breeding farm in northern England was closed off on Monday after an outbreak of bird flu, although officials said the risk to public health was "very low".
The Department for Environment, Food and Rural Affairs (Defra) said it might be linked to outbreaks in the Netherlands and Germany, but said it had yet to identify the strain, although the deadly H5N1 had been ruled out.
An estimated 6,000 ducks on the farm will be culled and a 10-kilometre (six-mile) restriction zone has been put around the site near Driffield in Yorkshire.
"We have confirmed a case of avian flu on a duck breeding farm in Yorkshire. The public health risk is very low and there is no risk to the food chain," a Defra statement said.
Any movement of poultry or products in or out of the protection zone is prohibited.
"Obviously we are asking farmers to be doubly vigilant in their biosecurity and look for any potential signs of disease in their flock," a spokesman added.
The government's chief veterinary officer, Nigel Gibbens, said officials were looking at any possible links to outbreaks of bird flu in the Netherlands and Germany.


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Having problems with your IBM NetVista PC Desktop ?
I have a ibm netvista 8319 44g vista upgrade was recommended BUT i can not adjust screen resolution. I have tried lots of different fixes seems i need a new graphics card but what is compatable. i have a budget of £50 ish wont be used for high end games
Answers :
Hmm, have you gotten the latest video driver from the manufacturer?
Also, What resolution are you running? and what resolution do you need to reach?
More info on the PC like Video Card/Monitor Make and models would be valuable.
Also, What resolution are you running? and what resolution do you need to reach?
More info on the PC like Video Card/Monitor Make and models would be valuable.
Repair Help & Product Troubleshooting for IBM NetVista PC Desktop
Tips for a great answer:- Provide details, support with references or personal experience .
- If you need clarification, ask it in the comment box .
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Suggested Questions/Answers :
I have a ibm netvista 8319 44g vista upgrade was recommended BUT i can not adjust screen resolution. I have tried lots of different fixes seems i need a new graphics card but what is compatable. i have a budget of £50 ish wont be used for high end games
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text:
I was looking around, and I don't see many cosplay gatherings where I live. I know there have been a few gatherings near Funway, (I live close by) but those were last year and I hadn't heard anything about them. I can't guarantee that I will go to a gathering, even if someone tells me about it, but at the very least I'd like to use this topic to help others who live in the area find gatherings!
There is going to be a gathering at Nakama Toys on July 5th. You can find it if you look on their facebook page or google it.
Page 1 of 1
Any Gatherings This Summer? 2014 Anyone want to have a cosplay gathering?
Posted 01 July 2014 - 02:52 PM
Posted 01 July 2014 - 05:45 PM
2014 ACen was my very first con, but now I plan on going to AI (Anime Iowa) in about a month =3 I don't know of many more cons besides Midwest furfest and stuff =3
Posted 02 July 2014 - 05:05 PM
I think there will be a few more cons in December. However, I was looking for gatherings. XD Sorry if I wasn't specific.
Posted 02 July 2014 - 05:10 PM
By the way, is that a costume you made on your avatar picture? I dressed as fem!Luffy in a dress for ACen 2014. ^^
Posted 02 July 2014 - 09:14 PM
Posted 06 July 2014 - 08:17 PM
That looks amazing!
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text: Contractual Consistency Between BON Static and Dynamic Diagrams Ali Taleghani July 30, 2004 1 Overview Model-Driven Development & Models Contractual Consistency – The Problem Previous Work Current Work – Semantics of Dynamic Diagrams BON Development Tool – BDT Contribution and Future Work 2 MDD & Models Model-Driven Development proposes development based on models Several views can be used to describe system Models must be executable, and views consistent Contributions Automated consistency checking Symbolic model execution 3 Contractual Consistency – Example ACCOUNT S cen ario 1: B an k E xam p le balance: IN T E G E R m ake ! balance = 0 PERSON 1. C reate A ccount 2. W ithdraw 200 w ithdraw (a: IN T E G E R ) ? balance > = a ! balance = old balance - a In varian t balance > = 0 PERSON 1, 2 ACCOUNT • SD contains contracts only – No implementation • Want to create account and withdraw $200 • make sets (balance = 0), but precondition of withdraw requires (balance >= 200) Contract Violation 4 Contractual Consistency SD and DD are the two views involved SD contains contracts only – no implementation Contracts are pre, postconditions and class invariants Views contractually consistent if messages in DD corresponding to routines in SD can be executed without contract violations 5 Previous Work Problem of consistency with contracts not extensively discussed –informal approaches only [Paige 2002] first to formalize problem Cites 4 criteria for checking consistency Last criteria is contractual consistency We add additional constraints for implementation 6 Semantics of Dynamic Diagram SSC i SSC ri i 1 • Message mi in DD is mapped to a feature ri in the target class in SD • Routine takes system from one system state constraint (SSCi) to the next (SSCi+1) • SSC represents a constraint on the attributes in the system • SSCi+1 constructed using SSCi and contracts of ri 7 Current Contribution - 1 Check Contractual Consistency using Symbolic Model Execution Define Symbolic Execution Step as execution of one message in DD Step ( SSC i , ri , SSC i 1 ) successful iff SSC i ri . pre Precondition of routine is satisfied SSC i 1 False SSC is not a contradiction 8 Current Contribution - 2 Views contractually consistent iff i 1 i n | Step ( SSC i , ri , SSC i 1 ) No implementation provided Require use of Theorem Prover Use Simplify from ESC/Java Automatic and Fast Returns counter example 9 BON Development Tool - BDT Static Diagramming Tool • Construct Class diagrams • Include contracts 10 BON Development Tool - BDT Dynamic Diagramming Tool • Draw objects and messages • Assign messages to routines from SD 11 BON Development Tool - BDT Consistency Tool • Specify an initial state constraint • Contract violation results in counter example • User can use counter example to make changes to contracts, messages 12 Comparison to Tool of [Gao2004] Gao's Tool Test drivers and implementation required Checks one or a few execution paths Complete (for that execution) BDT Automatic and no implementation required All execution paths starting in a state constraint are checked Incomplete since working with a theorem prover 13 Contribution First contractual consistency tool without the need to specify implementation Early symbolic execution of partial models Can use dynamic (collaboration) diagrams Use contracts only – higher level than MDD State Chart Action Languages Tool is user friendly Simplify works automatically under the hood Simplify works quickly 14 Future Work Work out theory for sub-messages in DD BDT Add invariants and inheritance Support quantifications Combine BDT with EDT for complete code generation Add support for program verification – using ERC 15 Thank You 16


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The Roots of Rafe's WrathMature
When Pontius leads me into the dining room, I find Rafe is already at the table, doing his level best to be the worst conversationalist ever. It's a full house tonight; not only is Woof here, but Pontius has invited both stylists and both prep teams to join us. Rafe keeps his face turned away from everyone and whenever he is asked a question he gives the shortest answer possible, if any at all.
'Ah! There's the moon to our sun!' Otho cries when he sees me and the others turn to greet me, too. The tall, ghostly man drifts over and offers me a glass of deep red wine, which I accept. He steals me away from Pontius and walks me to the table, where he introduces me to Rafe's prep team. There are two women and a man; Alba, Drusilla, and August. Everyone is full of praise for Lu and Otho's designs, and congratulations for Rafe's and my celebrity. Agrippa stands up and pulls out a chair for me next to his. I flash him a smile as I take my seat. I'm across from Rafe, who doesn't look happy about it.
Just like on the train, Woof is sat at the end of the table, adjacent to me and Rafe. 'I hope you didn't lose your appetite,' he says with a chuckle. 'Lu informs me you're on an all-you-can-eat diet between now and the interviews.'
Dinner commences. Everyone makes pleasant small talk throughout several courses. Pontius tells us about his day over pea soup with mint. Otho talks at length about how he and Lucretia came up with the chariot costumes as we eat salad with tiger prawns in a lemony sauce. Roasted mushrooms stuffed with cheese and garlic accompany August's gossip about people I don't know. I probe Agrippa on his love of miniature paintwork while we dine on glazed ham sliced wafer thin, served with bacon-wrapped asparagus and braised apples. The talk moves on to ideas for our interview outfits as we're served cheeses and grapes, then warm slices of a dark chocolate and black cherry torte. I have dutifully stuffed myself to the gills, with no help from Woof. Finally, when the table is cleared of everything but drinks and a bowl of fresh fruit, we get down to business.
'So,' says Woof, looking at Rafe, 'Let's talk strategy. What are your skills? What hobbies do you have that might have given you some edge?'
Rafe shrugs. 'I like to run. I'm pretty fast, I guess.'
Woof waits for him to go on, but when he doesn't, the victor nods. 'Okay, that's good. Speed is always useful in the Games. And you, Renore?'
Violet's voice whispers to me. Remember your dancing. There's got to be some way you can use that.
'I like to dance. I've been practising since I was little.'
Rafe snorts, but Woof gives me a shot to make my point. 'And what sort of skills would you say that gives you?'
'Fitness. Flexibility,' I begin slowly, thinking. 'Agility. Balance. Stamina. Stability. An eye for detail.' I grow more confident as my list grows longer. 'Creative thinking. Quick reflexes.'
Rafe decides to test that last one here and now. He snatches an apple out of the fruit bowl and lobs it at my head. I catch it in front of my face and lob it right back. It hits him square in the forehead, bounces off and, to my own astonishment (and immense contentment), I catch it again. My attacker looks understandably livid. I won't lie, I feel quite full of myself right now. I look Rafe dead in the eyes and take a slow, deliberate bite of the apple, filling the action with as much smugness and sexuality as I can muster.
It's all over before anyone can react. The Capitol folk look ruffled and Woof is clearing his throat in uncertainty. 'Ahem. Well, I guess that theory checks out. Now-'
But Rafe interrupts him. 'Wait. Let her finish. You haven't let her tell you about her other hobby.'
'Oh?' Woof raises his eyebrows in surprise. He looks curiously from Rafe to me. 'What would that be, then?'
He can't mean… No. Not here. Not in front of everyone. Tell Woof, that's fine. I can even stand Pontius knowing. But not Lu. Not Agrippa.
A horrible smile is spreading over Rafe's face. He looks like a predator preparing to taste the most delicious prey of his life. When I fail to speak, he answers for me.
'She's a whore.'
'Language!' gasps Pontius, above the whispers and murmurs breaking out around the table.
Woof coughs again. 'There's no need for name calling-'
This time, I interrupt. I'm not going to give Rafe the satisfaction of seeing my shame. That's what he wants. I'm not going to let him win. Since it's already out there, I'm going to wear it like armour. I still can't bring myself to look at Agrippa, but I keep my tone calm and casual as I say, 'No, it's true. I've been on the game since I was thirteen.'
This time, the table goes silent.
Then Woof, the wily old man, turns to me to ask, 'So… What useful skills might that have given you?'
I decide I love Woof.
I laugh. 'A lot of the same ones, I guess. Stamina, flexibility...' I wink. 'Creative thinking.'
Woof laughs, too. So do Agrippa and Lucretia. The others follow suit. Rafe doesn't look happy. Foiled again. A thought strikes me.
'You know, I suppose it's also where I developed my acting skills.'
'Oh,' Rafe's voice is deadly quiet. 'Is that how you could fuck those Peacekeeper pigs? Did you act like they didn't murder our parents, or did that just turn you on?'
Time skips.
Several chairs are toppled over on the floor. There are broken glasses and spilled drinks everywhere. I'm on the tabletop, aiming a kick at Rafe's hideous face. Otho and his prep team have retreated to a safe distance and are watching with horror, hands over mouths. Cassius and Felix are motionless, dumbstruck. Lucretia and Woof are trying to pull Rafe out of my boot's firing range.
Agrippa's hands clamp down on my arms from behind and he drags me off the table before I can strike. I'm kicking anyway. I want to tell Woof that I've changed my mind, that I don't want to be mentored with Rafe, but all I manage to get out is, 'Separate! Separate!' over and over as Agrippa hauls me out of the room and down the hall.


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Boston ROC Researchers present at ACRM Annual Conference
Boston ROC Co-Director Alan Jette, PhD, PT, and PRO Core Director Wendy Coster, PhD, OTR/L, FAOTA, along with colleagues David Tulsky, PhD, from the University of Michigan, Ann Arbor, MI and Vanessa Noonan, PT, PhD, Rick Hansen Institute, Vancouver, BC, CA held a symposium at this years ACRM annual conference in Vancouver, entitled "Development and Initial Evaluation of the SCI-CAT Functional Assessment Instrument."
This symposium presented the latest findings of a new functional measure for adults with spinal cord injury, called the Spinal Cord Injury Computer Adaptive Test (SCI-CAT). There is growing recognition that limitations of current SCI outcome measures pose a serious impediment to conducting SCI research and monitoring clinical programs. The SCI-CAT, is a state-of-the-art functional measure for adults with spinal cord injury,supported by the NIDRR National Model Systems Program for Spinal Cord Injury. Presenters discussed computer adaptive testing (CAT)methods, reviewed the development of the SCI-CAT measure, presented findings on the SCI-CAT's psychometric properties, and over-viewed ongoing efforts to link the SCI-CAT with a pediatric SCI functional assessment measure to enable the assessment of functional abilities across the lifespan. These innovative measures can be used to advance spinal cord injury research and clinical practice.
To read more about the development of the SCI-CAT instrument, two new papers were published in the Archives of Physical Medicine and Rehabilitation on the item bank development and initial evaluation.


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#824 - Steps to Water! Patio and Balconies!
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Bedrooms: 3/
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Sleeps: 11/
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Book this 3 bedroom, 3 bathroom house in Mission Beach today! A total of 11 people can sleep here comfortably.
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Fiorinal with Codeine
FDA Allows Marketing of First Device to Relieve Migraine Headache Pain »
"The U.S. Food and Drug Administration today allowed marketing of the Cerena Transcranial Magnetic Stimulator (TMS), the first device to relieve pain caused by migraine headaches that are preceded by an aura: a visual, sensory or motor disturbance"...
Read the FDA Allows Marketing of First Device to Relieve Migraine Headache Pain article »
Fiorinal with Codeine
Fiorinal with Codeine is indicated for the relief of the symptom complex of tension (or muscle contraction) headache.
Evidence supporting the efficacy of Fiorinal with Codeine is derived from 2 multi-clinic trials that compared patients with tension headache randomly assigned to 4 parallel treatments: Fiorinal with Codeine, codeine, Fiorinal (Butalbital, Aspirin, and Caffeine Capsules, USP), and placebo. Response was assessed over the course of the first 4 hours of each of 2 distinct headaches, separated by at least 24 hours. Fiorinal with Codeine proved statistically significantly superior to each of its components (Fiorinal, codeine) and to placebo on measures of pain relief.
Evidence supporting the efficacy and safety of Fiorinal with Codeine in the treatment of multiple recurrent headaches is unavailable. Caution in this regard is required because codeine and butalbital are habit-forming and potentially abusable.
One or 2 capsules every 4 hours. Total daily dosage should not exceed 6 capsules.
Extended and repeated use of this product is not recommended because of the potential for physical dependence.
Fiorinal® with Codeine (Butalbital, Aspirin, Caffeine, and Codeine Phosphate Capsules, USP)
Blue cap with a yellow body. Cap is imprinted twice with "FIORINAL" and "CODEINE" in red. Body is imprinted twice with "WATSON 956" in red.
Bottles of 100 are supplied
with child-resistant closures.
Store and Dispense
Below 25°C (77°F); tight container. Protect from moisture.
Keep out of reach of children.
Address medical inquiries to: WATSON Medical Communications, P.O. Box 1953 Morristown, NJ 07962-1953. 800-272-5525. Distributed By: Watson Pharma, Inc. Morristown, NJ 07962. Revised: May 2013This monograph has been modified to include the generic and brand name in many instances.
Last reviewed on RxList: 2/8/2017
Fiorinal with Codeine FDA Prescribing Information
Additional Fiorinal with Codeine Information
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You are encouraged to report negative side effects of prescription drugs to the FDA. Visit the FDA MedWatch website or call 1-800-FDA-1088.
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Japan, which headed the UN's Human Development Index league table in 1990, 1991 and 1993, has fallen back into third place. Canada last came top in 1992. Britain is listed as number 10, one place ahead of Germany.
Meanwhile, Guinea retains its unenviable status as the least-developed country in the world, as measured by the index. Life expectancy in the African state is 43.9 years, the adult literacy rate is 27 per cent, the average child spends less than one year of its life at school and Gross Domestic Product per person is dollars 500 ( pounds 333).
The index, first calculated in 1990, was yesterday published for the fourth year running along with the UN Development Programme's Human Development Report. It gives each country a value between one and zero based on the size of its economy per capita, the educational attainment of its citizens and how long they live. The index was devised because the UN felt money alone was not the best way to compare whether one country's people have a better quality of life than another's.
Although criticised for being simplistic, the index is enjoying growing popularity as a way of showing the vast and growing differences in the quality of life between countries. The accompanying report says that 60 per cent of the world's population now enjoy 'fairly satisfactory' human development levels with an index value above 0.6, compared to only 25 per cent in 1960.
It also finds hope in the fact that global military spending has fallen significantly in the past six years, and that more than one-half of the world's peoples 'live in relatively pluralistic and democratic regimes'.
----------------------------------------------------------------- HUMAN DEVELOPMENT INDEX ----------------------------------------------------------------- Value Rank Canada 0.932 1 Switzerland 0.931 2 Japan 0.929 3 Sweden 0.928 4 USA 0.925 8 United Kingdom 0.919 10 Germany 0.918 11 Afghanistan 0.208 171 Burkina Faso 0.203 172 Guinea 0.191 173 -----------------------------------------------------------------Reuse content


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48 acres in Mendocino County, California
California Ranch for Sale - This is a rare opportunity to own 48 +/- acre RV/Trailer Park located about 6 miles south of the Historic village of Mendocino. It sits at the mouth of the Albion River and goes up the river for over 3700 feet. This tidal influenced river is one of the few navigable rivers in the state. You can travel five miles up the river and enjoy the splendor of wildlife and the towering redwoods or head out to sea and fish for Salmon or Rock fish. Known as the Schooner Landing Park, It has 41 campsites with 25 full hook up sites with power, water, and sewer. There is a concrete boat ramp and docks. The property comes with three rental houses along the river and an owner's home on top of the ridge. This property is a recreation playground. You can run the RV/ Trailer park as an eco-tourism center with canoes and kayaks that take people up the river and view the beautiful river surroundings It would also make a great family compound, Church retreat, or educational facilities
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In town for the Cash Ba$h. Had to grab a few more caches Sunday morning before heading home. Once again this was a great park to cache in. Enjoyed the theme here. TFTC!
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New Braunfels Herald Zeitung Newspaper Archives
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New Braunfels Herald Zeitung (Newspaper) - December 23, 2000, New Braunfels, Texas Page 8A — Herald-Zbitung — Saturday, December 23, 2000SERVICES/From 7A Religion Briefs candlelight service planned for 6:30 p.m. Sunday. Sts. Peter and Paul Catholic Church will celebrate a Children's Christmas Mass at 5 p.m., a Christmas Mass at 7 p.m. and Midnight Mass at 12 p.m., all on Sunday. On Monday, Sts. Peter and Paul will celebrate Christmas Mass at 8 a.m., 9:30 a.m. and 11:15 a.m. Cross Lutheran Church, 169 S. Hickory, will conduct a Christmas service at 8:45 a.m. and 11:15 a.m. and 5 p.m., 7 p.m. and 11 p.m. Sunday, and IO a.m. Monday. St. John's Episcopal Church, 312 S. Guenther, has scheduled Family Christmas Eve services for 9:15 a.m. and 5:30 p.m. with a candlelight service at 10:30 a.m. Tree of Life Church, 652 Loop 337, will have Sunday services at the usual times, 8:30 a.m. and 10:30 a.m. "We'll be singing traditional Christmas songs," said Beatrice Gonzales, pastor's secretary. "Come join us as we hear the message of how God sent His Son to have relationship with us." cession scheduled with live Mary and Joseph carrying Baby Jesus. Grace Memorial Church hosts a 5 p.m. Sunday Christmas church service at Memorial Primary School, 1200 County Line Rd. First Baptist Church, 733 Cross St., will conduct a Christmas Eve candlelight service at 5 p.m. "We'll sing some Christmas hymns and we'll have the Lord's Supper," said Cynthia Heierman, receptionist at First Baptist Church. Faith United Church of Christ, 970 Loop 337 N., has scheduled a Christmas carol service for 5:30 p.m. Sunday. Peace Lutheran Church has scheduled Christmas services for 8 and 10:30 a.m. and 7 p.m. Sunday. First United Methodist Church, 572 W. San Antonio St., will celebrate the birth of Jesus at 5, 7, 8:30 and 11 p.m. Sunday. Canyon Lake United Methodist Church will conduct a Christmas Eve candlelight service at 7 p.m. Community Christian Church, 1750 Old McQueeney Rd., has a Colo., to do mission work with the Denver Urban Ministries. According to FUMC Youth Director, Steve Peyton, DenUM serves the inner city area of Denver by providing for the homeless, low-income child care, homebound food delivery and other inner city needs. The youth will participate in some of these programs as a supervised experience. The youth are excited to give of themselves and serve others, especially during the holiday season when the less fortunate can easily be forgotten. They plan to return Jan. 2. 6:30-7:45 p.m. Tuesday-—Yoga Class- 8:30-9:45 a.m. (Except for 2nd Monday and following Tuesday of the month) Tuesday—Reiki Healing— 6:30-8:00 p.m. (Love Offering) Thursday—A Course in Miracles-6:30-8:00 p.m. (Love Offering) (2nd Thurs. is Sit-up tune-up (Chair Massage)/ Health Issues) year 2001. St. John's presently supports missionaries in South America and the Caribbean, Russia, Africa and India.Bracken hosts Christmas services Bracken United Methodist Church, 20377 FM 2252 (Nacogdoches Rd.) I mile northeast of FM 3009 (Natural Bridge Caverns Rd.) hosts a 5 p.m. fami ly worship and 7 p.m. and 11 p.m. candlelight services on Christmas Eve. For information, call 606-6717.New group opens at church The Unity Church of Practical Christianity announces a new 11th step open AA/Al-Anon meeting at 5 p.m. Jan. 7 at the church, 642 Comal Ave.,with the Canterbury New Braunfels Group. The group will study and discuss Emmett Fox's Sermon on the Mount. At the first meeting, the group will discuss experiences with the preface and first section entitled, "What did Jesus teach?" For information, call 608-9515.Church presents Christmas pageant The Unity Church located on State Highway 123 will present its Christmas pageant, "In the Company of Angels," at 11 a.m. Sunday. Refreshments will be served after the production. The church's traditional candle lighting service takes place at 6:30 p.m. Sunday evening. The Unity Church is located 4.7 miles east IH-35 and 15 miles northwest of Seguin on State Highway 123. For information, call (512) 392-5199.St. John's says 'thank you' St. John's Episcopal Church wishes to acknowledge and thank all those in New Braunfels, Seguin and San Antonio who contributed to the success of Mission Mania Sunday. The event included presentations by missionaries, a silent auction and a barbecue dinner. The hearts of many people were stirred to serve the mission field, and IOO percent of the funds raised will support those who are serving and who will serve in theUnity Church announces events Unity Church of Practical Christianity, 642 Comal Ave., presents an 11 a.m. service on Sunday. The program will be "A Christmas Blessing." All are welcome. For information, call 608-9515. Regular weekly events include Monday— Yoga Class-Youth prepare for Denver trip On Tuesday, IO youth and three adults from the First United Methodist Church in New Braunfels will travel to Denver, I v. St. Andrew Lutheran Church Pastor Jere Kimmell 7420 FM 2673,1 mile E. of Starkville. Sunday worship 8 a.m. Sc 10:15 a.m. Sunday school, 9 a.m., Nursery provided. St. John's Episcopal Church 312 S. Guenther Ave. Holy Eucharist Rite One 8 a.m., Sunday school 9:15 & 10:30 a.m. Holy Eucharist Rite Two 9:15 & 10:30 a.m. St. Joseph's Anglican Church 446 N. Seguin 11 a.m.-Sunday worship 7 p.m.-Tuesday bible study 7 p.m.-Wcdnesday evening prayer "historic faith" "Traditional worship" St. Paul Lutheran Church (ELCA), Cross and Santa Clara Streets, Sunday worship 8 a.m., (broadcast on KNBT radio FM 92), 10:30 a.m., Sunday school 9:15 a.m. St. Paul United Church or Christ 108 S. Main St. at Loop 539 Cibolo, Tx. 78108 Pastor Bren Becker IO am, Church services Sc Sunday school 210-658-5874 "Where the past, present SI future may be one." Sts. Peter And Paul Catholic Church 386 N. Castell, 625-4531. Monsignor Eugene O'Callaghan. Mass: SaL 5 pm; Sun. 8 am., 9-J0 am, & 11:15 am Mom-Thurs., 7 am, Fri. 8 am St. Thomas Catholic Church 180 St. Thomas Dr. Canyon Lake, Texas Monsignor Marvin G. Doerfler Mass:Saturday 6 p.m.;Sunday 8:30 a.m. Sc 11:00 a.m.; Wednesday - Friday 8:30 a.m. 830-964-3497 Tree Or Lire Church 652 Loop 337. Sunday services (including Children's church) at 8:30 arn., 10:30 a.m. and 7:00 p.m.; Ladies bible study IO a.m. Wednesday; Wednesday services 7:00 p.m. Youth Meeting 7 p.m. Wednesday The Unitarian Universalists Or Comal County Meet on Wednesday at the Faith United Church of Christ, 970 N. Loop 337, at 7:30 p.m. 629-4262. Public invited. Unity Center Or Practical Christianity In San Marcos 16870 N. State Hwy. 123, San Marcos, TX 78666. (512) 392-5199. Sunday 9:30 am. Bible class, 11 a.m. Worship; Tuesday 11 am. Prayer services Unity Church Or Practical Christianity 642 Comal St. Sunday service 11:00 a.m. Children's service Sc Child care 11:00 am. Course in miracles Thursday 6:30 p.m. Reiki Tuesday, 6:30 pm. Information: 608-9515 Victory Temple Church 134 Deborah Dr. New Braunfels, Tx. 830-608-1300. Pentecostal/interdenominational Sunday worship IO am. Sc 6 pm. Children's church Sun. 10:30 am. Pastor Ron Womack Dial-A-Prayer: 512-396-LIFE (5433). Sondra Von Cyllenbad spiritual leader. Call for weekly class schedule. Friedens United Church or Christ 830-303-7729 2555 Friedens Church Road Seguin (Geronimo), Texas. Sunday school at 9 a.m. Sunday worship at 10:15 am. Foursquare Gospel 430 FM 306,606-2843. Pastor Fred McMichael. Sunday school IO arn., Sunday worship 11 arn.; Wed. 7 p.m. Greater Lite United Pentecostal Church 134 Enchantment Lane, off FM 725. Pastor Jack Dool; Sunday services IO a.m. and 6 pm. Wed. 7:30 pm. 609-1325. Harmony Baptist Church 1472 Hwy. 46 South. Bible study at 10 am.-Sunday, worship: 11 am. Sc 6 pm.; Wednesday prayer Sc Bible study ar 7 pm Hiu Country Christian Church Adon Seay School, Hwy. 46 West Sunday 10:30 Contemporaty Worship Holy Family Catholic Church 245 S. Hidalgo, 609-5320. Masses: Spanish, Mon, Thurs, Sc Sun. 8 am English,Wed. Sc Fri. 8 am, Sat. 5 pm, Sun.IO am & 12 pm Confessions 4-4:45 pm or by appt. Father James Wasser, M.S.F. Holy Ground Christian Church 210-659-4408 Services @ Stone Creek RV Park Rec. Hall 18905 IH 35 N @ Exit 177S Schertz 78154 Pastor Darin Sanders Wed.: Fellowship 6 p.m. Bible Study 7pm. Sun.Worship Services: 10 a.m. Iglesia Bethel Pentecostes 385 N. Grape Prayer meetings- Monday 7:30 p.m.Wed & Sat 7 pm. Sun. 6 pm Sunday school IO am; Sunday worship 11 am. Children's church - Sunday mom Sc eve. Pastor Frank Llamaz 623-6113 Iglesia Dc Cristo 134 Meadow Crest, #102. 625-1020. 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|Xiang Jia Min*
|Center for Applied Chemical Biology, Department of Biological Sciences, Youngstown State University, youngstown, OH 44555, USA
|Corresponding Author :
|Xiang Jia
Min, Center for Applied Chemical Biology,
Department of Biological Sciences,
Youngstown State University,
Youngstown, OH 44555, USA,
E-mail: email@example.com
|Received March 15, 2010; Accepted April 21, 2010; Published April 21, 2010
|Citation: Min XJ (2010) Evaluation of Computational Methods for Secreted Protein Prediction in Different Eukaryotes. J Proteomics Bioinform 3: 143-147. doi:10.4172/jpb.1000133
|Copyright: © 2010 Min XJ. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Secreted proteins play important biological roles in eukaryotes. Computational identification of all secreted proteins, i. e. the secretome, from predicted proteome of completely sequenced genomes is an essential step in functional annotation. To develop screening methods for secreted proteins in different kingdoms of eukaryotes, we have evaluated the prediction accuracies of SignalP, Phobius, TargetP, and WolfPsort used individually or in combination with TMHMM and PS-Scan. Prediction accuracy was represented by Mathews' Correlation Coefficient (MCC). The tools show different strength for predicting secreted proteins in different kingdoms of eukaryotes. When individual tools were used, we found that the tools having the highest accuracy were WolfPsort for fungi (73.1%), Phobius for animals (82.8%), SignalP for plants (55.4%), and Phobius for protists (42.1%), respectively. Except using Phobius, combining the prediction tools with TMHMM significantly improved the prediction accuracy in all data sets. Based on the measured accuracies, it is recommended that using the following methods for secretome prediction in different eukaryotes: SignalP/TMHMM/ WolfPsort/Phobius/PS-Scan for fungi (83.4%), Phobius/WolfPsort/PS-Scan for animals (86.7%), SignalP/TMHMM/ Phobius/TargetP/PS-Scan for plants (73.2%), and combining all the tools for protists (52.8%).
|Secreted proteins; Secreteome; Prediction; Signal peptide; Method
|Eukaryotic cells make thousands of different proteins. Among them some proteins function only after they are secreted from the cell. These secreted proteins play critical biological roles. For example, fungi secrete enzymes to break down potential food sources (Tsang et al., 2009); plant secreted proteins are primarily part of cell wall proteome (Jamet et al., 2008); human secreted proteins are involved in cellular immunity and communication and provides useful information for the discovery of novel biomarkers such as for cancer diagnosis (Hathout, 2007; Xue et al., 2008).
|Almost all eukaryotic secreted proteins contain a signal peptide at the N-terminus that directs proteins to the rough ER and the Golgi complex (Blobel and Dobberstein, 1975; von Heijne, 1990). The signal peptide, typically 15 – 30 amino acids long, is cleaved off during translocation across the membrane. While some proteins without an N-terminal signal peptide can be found in the ER and the Golgi, over 90% of human secreted proteins contain classical N-terminal signal peptides (Scott et al., 2004). A number of computational tools have been developed for predicting subcellular locations of proteins including secretion to the extracellular space (Emanuelsson et al., 2007). Among the computational tools, SignalP (Bendtsen et al., 2004) in conjunction with TMHMM (http://www.cbs.dtu.dk/services/TMHMM/), TargetP (Emanuelsson et al., 2000; Emanuelsson et al., 2007), and Phobius (Kall et al., 2004; Kall et al., 2007) were widely applied to genomewide prediction of all secreted proteins in an organism, i. e. putative secretomes; and some of the predicted secreted proteins were experimentally validated using proteomics approaches (Wymelenberg et al., 2005; Tsang et al., 2009). For example, secretomes are analyzed in the nematode Nippostrongylus brasiliensis (Harcus et al., 2004) and the vetigastropod Haliotis asinina (Jackson et al., 2006) using SignalP, human, puffer fish, and pig using TargetP (Klee et al., 2004), and zebrafish using SignalP/TargetP/Phobius/pTarget (Klee, 2008). A number of fungal secretomes including Candida albicans (Lee et al., 2003), Phanerochaete chrysosporium (Wymelenberg et al., 2005), and Aspergillus niger (Tsang et al., 2009) were predicted using SignalP/TMHMM/TargetP method.
|The quality of secretome prediction depends primarily on the accuracy of the computational tools. The prediction performances of some earlier versions of these tools mentioned above have been evaluated (Menne et al., 2000). Klee and Ellis, (2005) evaluated SignalP (version 2.0 and 3.0), TargetP (version 1.01), Phobius, PrediSi, and ProtComp using mammalian data extracted from SwissProt database and proposed to use TargetP, SignalP 2.0 maximum Y-score, and SignalP 3.0 maximum S-score to increase accuracy (Klee and Ellis, 2005). Chen et al. (2003) compared CJ-SPHMM/TMHMM with PSORT and found that combing these tools improved the prediction accuracy and subsequently used this method to construct a secreted protein database for human, mouse and rat (Chen et al., 2003; Chen et al., 2005). O'Toole et al. (2006) compared SignalP/TMHMM with Phobius using manually curated Saccharomyces cerevisiae data in the SGD database (http://www.yeastgenome.org/) and found Phobius predicted secreted proteins with higher sensitivity and specificity than using SignalP/TMHMM (O'Toole et al., 2006). However, Tsang et al. (2009) recently showed that the prediction of secreted proteins using SignalP/TMHMM results in higher specificity than Phobius in A. niger experimental data (Tsang et al., 2009). In addition, they also observed that the combined results from the Phobius and SignalP/TMHMM protocols yielded higher specificity than these protocols were used separately (Tsang et al., 2009).
|Taken together, there is a need for comparative assessment of the prediction accuracies of computational tools, particularly the combination of these tools, for organisms in specific kingdoms of eukaryotes. In order to develop computational methods for secretome prediction from species in different kingdoms of eukaryotes, we have evaluated the accuracies of SignalP, Phobius, TargetP, and a new version of PSort – WolfPsort (Horton et al., 2007), and their synergistic performances in conjunction with TMHMM and PS-Scan (http://www.expasy. org/tools/scanprosite/) using manually curated data of fungi, animals, plants and protists in Swiss-Prot database. PS-Scan was included to remove ER targeted proteins (de Castro et al., 2006).
|Data and Methods
|Manually annotated secreted (positives) and nonsecreted (negatives) proteins in eukaryotes were extracted from UniProt- SwissProt database (UniProt release 15.4; June 2009) (http://www.ebi.ac.uk/uniprot/database/download.html). The positive dataset was extracted from entries annotated as "secreted" or "extracellular" in the subcellular location. The negative dataset includes all entries with a subcellular location in membrane, endoplasmic reticulum (ER), cytoplasm, nucleus, mitochondrion, etc. Entries annotated both as "secreted" or "extracellular" and also located in one or more other subcellular locations were eliminated. Entries in the subcellular location annotated as "Putative", "Potential", "Possible", or "By similarity" were also eliminated. Protein sequences not starting with methionine (M) or having less than 50 amino acids were eliminated. The dataset was subdivided into four datasets consisting of 241 secreted proteins and 5,992 nonsecreted proteins in fungi, 5,568 secreted proteins and 19,048 nonsecreted proteins in animals including human (metazoan), 216 secreted proteins and 7,528 nonsecrected proteins in plants, 32 secreted proteins and 1,979 nonsecreted proteins in protests, respectrespectively. The data are available upon request from the author.
|Four secreted protein predictors including SignalP (version 3.0) (Bendtsen et al., 2004), Phobius (Kall et al., 2004; Kall et al., 2007), TargetP (Emanuelsson et al., 2000), and WolfPsort (Horton et al., 2007; Sprenger et al., 2006) were selected as they were favourably evaluated previously (Klee and Ellis, 2005) and were widely used in the work mentioned above (see the references in Introduction). To improve the accuracy for secreted protein prediction, we also included TMHMM and PSScan. TMHMM was used to identify membrane proteins and was often used in tandem with SignalP (Emanuelsson et al., 2007; Tsang et al., 2009). PS-Scan was used to scan ER targeting sequence (Prosite: PS00014) (de Castro et al., 2006; O'Toole et al., 2006). Standalone-for-Linux version of SignalP (version 3.0) (http://www.cbs.dtu.dk/services/SignalP/), Phobius (http://phobius.binf.ku.dk/), TargetP (http://www.cbs.dtu.dk/services/TargetP/), WolfPsort (http://wolfpsort.org/), TMHMM (http:// www.cbs.dtu.dk/services/TMHMM), and PS-Scan (http://www. expasy.org/tools/scanprosite/) were obtained from the authors or downloaded from available websites. The default parameters were used for all the programs, except for processing protist data set as no protest specific parameters were available, we found TargetP performed better using the non-plant parameters and WolfPsort performed better using the fungal parameters. For SignalP prediction, only entries that are predicted to have a "mostly likely cleavage site" by SignalP-NN algorithm and a &signal peptide" by SignalP-HMM algorithm are considered to be true "positives" (Bendtsen et al., 2004) using the N-terminal 70 amino acids. For predicting membrane proteins using TMHMM, the entries having membrane domains not located within the N-terminus (the first 70 amino acids) were treated as real membrane proteins since our analysis showed treating entries having a single transmembrane domain located in the N-terminus significantly decreased the prediction accuracy. The performance of each individual prediction tools and various combinations of these tools was measured using prediction sensitivity (Equation 1), specificity (Equation 2) and Mathews' Correlation Coefficient (MCC) (Equation 3) (Matthews, 1975; Baldi et al., 2000; Menne et al., 2000).
Sensitivity (%) = TP/(TP + FN) x 100 (1)
Specificity (%) = TN/(TN + FP) x 100 (2)MCC (%) = (TP x TN – FP x FN) x 100 /((TP + FP) (TP + FN) (TN + FP) (TN + FN))1/2 (3)
|Where TP is the number of true positives, FN is the number of false negatives, FP is the number of false positives, and TN is the number of true negatives. When multiple tools were used, only the entries predicted to be positives by all the tools were taken as true positives.
|The performances of each individual prediction tool, combining with THMM, and combining with PS-Scan were evaluated in four datasets separately. The sensitivity, specificity, and Mathews' Correlation Coefficient were reported in Table 1 – 4. Because the sensitivity and the specificity were almost always in opposite trend, we used the MCC value to represent prediction accuracy of each individual tool or the combination of the tools.
|Prediction of fungal data
|Prediction accuracies for fungal secreted proteins are shown in Table 1. For individual tools, based on the MCC values, the performance is in the order of WolfPsort > Phobius > SignalP > TargetP. When TMHMM was used in tandem after these individual tools, the accuracies were substantially improved in SignalP/TMHMM (11.9% increment in MCC) and TargetP/TMHMM (14.9% increment in MCC). However, using TMHMM only slightly improved the performance of WolfPsort, and no improvement was observed in Phobius. Phobius has incorporated an algorithm to eliminate membrane proteins (Kall et al., 2004; Kall et al., 2007), thus the result was expected. The highest accuracy was achieved by combining SignalP, WolfPsort, and Phobius for signal peptide prediction, TMHMM for eliminating membrane proteins, and PS-Scan for removing ER targeting proteins (Table 1). However, adding TargetP to the above pipeline slightly reduced the accuracy, thus it is not recommended for processing fungal data. Our results are consistent with experimental validation in A. niger that reported higher specificity of secreted proteins was generated when combing SignalP/TMHMM with Phobius than when used individually, and TargetP erroneously assigned some secreted proteins to mitochondrion (Tsang et al., 2009).
|Prediction of animal data
|Prediction accuracies for secreted proteins in animals are shown in Table 2. For individual tools, based on the MCC values, the performance is in the order of Phobius > WolfPsort > SignalP > TargetP. When TMHMM was used in tandem after these individual tools, the accuracies were substantially improved in SignalP/TMHMM (18.1% increment in MCC) and TargetP/ TMHMM (25.3% increment in MCC). Using TMHMM following WolfPsort was also improved the prediction accuracy (5.5% increment in MCC) using TMHMM. However, when Phobius and TMHMM were combined, there was no improvement in prediction accuracy. Combining the top two prediction tools, Phobius and WolfPsort, and then PS-Scan further improved the prediction accuracy to a MCC value of 86.7%. Though adding TargetP and TMHMM to the pipeline further increased the accuracy to 86.9% (Table 2), for most of the applications, the method of combining Phobius/WolfPsort/PS-Scan would be adequate for predicting secreted proteins in animals. Certainly there is a trade-off between adding more tools for improving the accuracy and the efficiency for using less number of tools for processing animal data.
|Prediction of plant data
|Prediction accuracies for secreted proteins in plants are shown in Table 3. The performance of the individual tools is in the order of SignalP > WolfPsort > TargetP > Phobius. When individual tools are used, none of them had an accuracy of > 60%. Phobius has the lowest accuracy and thus was not suitable for predicting plant secreted proteins. WolfPsort had the highest specificity and the lowest sensitivity. When TMHMM was used in tandem after these individual tools, the accuracies were slightly improved in SignalP/TMHMM (7.6% increment in MCC), TargetP/TMHMM (9.8% increment in MCC), and WolfPsort/TMHMM (3.8%). The highest accuracy was achieved by combining SignalP, Phobius, TargetP, TMHMM and PS-Scan (Table3). This method thus is recommended for predicting secreted proteins in plants. Adding WolfPsort to the above pipeline reduced the accuracy as it significantly reduced the sensitivity and only slightly improved the specificity.
|Prediction of protist data
|Comparing with the number of curated secreted proteins in other eukaryotes, there are relatively much less data in protists. TargetP was trained for plants and non-plants, and we found it performed better using the non-plant parameter for secreted protein prediction in protists. WolfPsort was trained for fungi, animals, and plants. We found it performed best with fungal parameter for protist prediction. Prediction accuracies for secreted proteins in protists are shown in Table 4. Overall, all the programs performed poorly for secreted protein prediction in protists as all the MCC values are lower than 50%. However, based on the MCC values, the performance of the tools can still be assessed, and the order of the accuracies was Phobius > WolfPsort > SignalP> TargetP. SignalP/TMHMM performed best when used in tandem. The highest accuracy was achieved by combining SignalP, Phobius, TargetP, WolfPsort, TMHMM, and PS-Scan (Table3). It should be noted that even combining all these tools, the MCC value was only 52.8%, much lower than MCC values obtained for other eukaryotes. Clearly, more experimental data need to be collected for improving the prediction tools. Our evaluation was consistent with recentfindings that reported SignalP failed to predict a significant portion of secreted proteins in Plasmodium falciparum, a protozoan parasite that causes malaria in humans (van Ooij et al., 2008).
|Among the tools including SignalP, Phobius, TargetP, and WolfPsort, evaluated in this work for secreted protein prediction, the predictive accuracy of all these tools was much lower than those reported in the original publications, as was also found by Klee and Ellis, (2005). However, this comparison study has determined which tool, among the widely used prediction tools, had the highest accuracy for prediction of secreted proteins in different kingdoms of eukaryotes; and based on these results, the optimal computational method of combing different tools for each kingdom of eukaryotes was proposed. The data shows that when a single prediction tool was used or combined with TMHMM, different tools have different strength in processing different kingdoms of organisms. For fungal prediction WolfPsort/TMHMM was most accurate, and also SignalP/ TMHMM performed better than Phobius (Table 1), even though Phobius was originally designed to perform better than SignalP/ TMHMM (Kall et al., 2004; Kall et al., 2007). However, Phobius had the highest accuracy for processing animal data, though it was only marginally better when other tools used in tandem with TMHMM (Table 2). For plant secreted protein prediction, SignalP/TMHMM had the highest accuracy and Phobius was the worst predictor (Table 3). Among the tools evaluated for protist data processing, no any single tool and even combined with THHMM had a MCC accuracy value higher than 50% (Table 4).
|This was primarily due to the limit of available secreted protein data in protists that prevented adequate training for these tools.
|Combing multiple tools, however, often substantially improves the accuracy of secreted protein prediction (Chen et al., 2003; Klee and Ellis, 2005; Tsang et al., 2009). Adding SignalP/Phobius/PS-Scan to WolfPsort/TMHMM increased 7.9% in accuracy in fungi, adding WolfPsort/PS-Scan to Phobius increased 3.9% in accuracy in animals, adding Phobius/TargetP/ PS-Scan to SignalP/TMHMM increased 10.2% in plants (Table 1-3). Even in protists, when all the tools combined, we see 8.7% improvement in accuracy over SignalP/TMHMM method (Table 4). However, adding more prediction tools without discretion may decrease the prediction accuracy. In this work we found that adding TargetP in the method for fungi or WolfPsort in the prediction methods for plants reduced the prediction accuracy (Table 1, Table 3).
|In summary, among the tools evaluated in this work including SignalP, Phobius, TargetP, and WolfPsort, there was no single individual tool suitable for accurately predicting secreted proteins in all eukaryotes. If a single tool is used, WolfPsort is recommended for processing fungal data, Phobius for processing animal data, SignalP for processing plant data. TMHMM used in tandem with SignalP or WolfPsort significantly improved the prediction accuracies in all data sets. PS-Scan was useful to remove ER target proteins. More experimental data need to be collected for protists. The accuracy, particularly the specificity, however, is much improved when the two or more tools are differentially combined for predicting secreted proteins in different eukaryotes.
|We thank Gary Walker and Chest Cooper for their helpful comments on the paper. This work was supported by the Youngstown State University Research Council grant (2009-10 #04-10), Research Professorship, and the STEM Dean's reassigned time for research.
|Table 1
|Table 2
|Table 3
|Table 4


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Archived Listing - Other, RM 302,256
Other, For Sale at RM 302,256
is no longer active which means that the listing has expired or the property has been taken off our search result listings.
Active listings that fulfill similar criteria can be viewed towards the end of this page.
RM 18 psf
- Asking Price : RM 302,256
- Asking Price psf: : RM 18 per sq. ft.
- Occupancy : Vacant
- Posted Date : 03/12/2012
Description :
Bungalow land located at bukit tinggi for urgent sale. LA 16+k on hill top .near amenities and cozy area. asking 18 per sq ft nego. interested pls call john 016-9715819 viewing can be arrange.
Recent Sales Transactions
Average Price
Average Price Psf
How this was calculated
How this was calculated
Rental Yield
Rental Price Trend
How this was calculated
John OhREN 07002
016-971......Click to view number


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ECB's Draghi: comments
It seems that the ECB is in the wait-and-see mode.
- Risks remain to the downside.
- Rates will stay low for 'extended period of time' as long as inflation remains subdued.
- Inflation rates are expected to temporarily fall in the coming months.
- Current expectations of rate hikes in money markets are unwarranted.
- Credit trends to improve, with a lag.
Draghi gives both relatively dovish and hawkish comments.


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Written on: 10/12/2012 by simplyfab (1 review written)
I had gone to enquire about the facilities and the packages,the manager gave me a 5-day trial pass and signed a membership assuring that the entire money will be refunded in case I do not wish to continue.After the trial period,when I cancelled my membership I had to run behind them for more than a month to get the refund cheque, and then i did not get back the full amount.Now I am still chasing for this.Don't I have any other work?This is the worst service I have ever got from a bunch of cheats and liars.
Written on: 12/12/2012
Can we help? please email email@example.com and we will look into this. Thankso
As rated by our community of reviewers
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" Going downhill - Aylesbury " Read More
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" AVOID AT ALL COSTS. " Read More
" Unsafe Putney, poor management " Read More


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Quick Details
- Place of Origin: China (Mainland)
- Brand Name: LANJING
- Model Number: LJBB-002
- Hull Material: pvc tarpaulin
- Certification: CE
- Capacity (Person): 4-8
- Length: 4-6M, 4.7M
- Width: 205cm
- color: bule yellow
- material: 0.9mm pvc tarpaulin
- MOQ: 1 pc
- warranty: one year
- delivery time: 3-5 days
- in 5 days certificate: CE/UL Pump, with EN71,EN14960
- Gross weight: 43KG
- Use: Drifting
Packaging & Delivery
|Packaging Details:
|pvc bag and carton
|Delivery Detail:
|in 15 days
Specificationsbanana boat:
1, size:5.4x1.2m,6 seater
2,0.9mm pvc tarpaulin
4,full colors available
Inflatable Banana Boat
*The material is good.Air buoyancy tire still maintain good air tightness under 0.3bar pressure of work, this buoyancy tube, boat inside keel and floor have close combination, which makes inflatable boat enough rigidity, and ensures solid and driving rapidity.
*Boat bottom (not including fiberglass bottom) choose adhesive material with anti-reef performance, the possibility of damage to the bottom is reduced to small range.
*All the air buoyancy tire is divided into several independent air chambers, the segmentation set has high air tightness, when an individual chamber is damaged, the other parts remain enough buoyancy, the whole boat has the very good floatability. According to the buoyancy and space requirements, when a room or two adjacent chambers, or all of the chambers are damaged, the boat still has some buoyancy for emergency treatment in time
*With enough buoyancy, the whole boat already in the maximum load, even with water full of the whole boat, still can float on the surface of water.
*Due to buoyancy tires on both sides, lateral heel into water may be a small degree,and we have special stability test security among the product design and manufacture , so even in maximum load (even if there are large waves), it won't lateral heel into water. In often use, all quota crew focus on one side of the buoyancy tire, the boat will not overturned, and still can turn around with normal speed.
*The main accessories,such as steel drag ring, rescue rope components, boat-lift handles, oar-socket, and transom components are a guarantee of strength.
*Due to the high and low temperature resistance of fabric, corrosion resistance, anti-aging, and tight adhesiveness, inflatable boat is available five to eight years in the correct use and storage conditions, and maintenance is very convenient.