text:
A poll by Reuters of leading forecasters earlier this month found that the majority in the bull camp expect the benchmark Standard & Poor's 500 to end the year at about 1300, about 11% above its close last Thursday. Long-time bulls, such as Abby Joseph Cohen of Goldman Sachs, say the S&P 500 should be in the range of 1300 to 1425 by the end of next year. However, she predicted healthy gains this year and in 2000 which failed to materialise.
Other strategists believe the recent revival in equity prices, particularly in hi-tech shares, is unsustainable. Corporate profits, they say, are unlikely to turn around as quickly as the market expects. Merrill Lynch said that operating earnings per share for S&P 500 companies will be up a modest 13% next year, compared with a 32% decline in 2001. Investors are ignoring stable-growth companies with quality assets and instead are making 'big bets on cyclical companies on the theme that they will inevitably turn up', Richard Bernstein, Merrill's US quantitative market strategist, said. 'It shows in how much discussion there is about semiconductors and how little there is about food.'
Some strategists are suggesting investors stick with consumer staples and utilities, which are relatively undervalued when compared with 'sexier' industries, such as technology and telecommunications. JP Morgan's Doug Cliggott sees no relief in 2002. Wall Street's leading bear says the S&P will fall 19% next year and is encouraging investors to take advantage of the yearend rally to reduce exposure to the US.
- More about:
- Reuters


text:
Posted by Hugo Wetterberg on
Http Client is a library for making Http-requests. It has pluggable authentication methods and data formats.
Comes with support for authenticating with OAuth.module version 3.x for easy to access OAuth-protected resources.
Http Client has extensible support for different request and response body formats. That lets you send PHP data structures and have them serialized and deserialized automatically. JSON and serialized PHP is supported out of the box. This makes it a good companion to REST servers and very well suited for use with the version 2+ of the REST Server.
Required modules
- Autoload (Only for Drupal 6)
Optional modules
- OAuth 3.x
Project Information
- Maintenance status: Actively maintained
- Development status: Under active development
- Module categories: Third-party Integration
- Reported installs: 11,351 sites currently report using this module. View usage statistics.
- Downloads: 159,021
- Last modified: 2 December 2014
- Stable releases for this project are covered by the security advisory policy.
Look for the shield icon below.
|tar.gz (14.66 KB) | zip (18.11 KB)

subset: commoncrawl


text:
Salomon Upgrades Ford to 'Buy'
Analyst Michael Ward sees a ripe buying opportunity amid the car maker's tire controversy
Salomon Smith Barney upgraded Ford Motor Co. (F ) to buy from neutral.
Analyst Michael Ward believes the negative news surrounding the Firestone tire replacement program has knocked the stock down to attractive levels. He says at current levels, Ford's stock yield exceeds 5%, or a 43% premium to the 90-day T-bill yield. Over the last 15 years, Ford's yield has reached those levels only three times; each case proved to a be good buying opportunity. He says U.S. car/light truck sales have declined for eight consecutive months, and he thinks a downturn could last 24 months. In the past, the ideal time to buy auto-related stocks has been 9-12 months before the trough. Ward has a $36 12-month target.
Before it's here, it's on the Bloomberg Terminal. LEARN MORE


text:
|Story Views
|Last Hour:
|Last 24 Hours:
Pomegranate Market Production, Supply, Sales, and Demand Research Report is a systematically conducted exhaustive study of the Pomegranate market. The aim of the Pomegranate Industry report is to assist novice as well as seeded players in gaining astute business intelligence and make apt decisions based on it.
Detailed TOC and Charts & Tables of Pomegranate Market Research Report available at- http://www.absolutereports.com/global-pomegranate-market-research-report-2016-10395870
The analysis in the Pomegranate Market report is of a two-fold perspective, production and sales. This commercial study encompasses detailed investigation of production, supply, sales, demand, price, cost, income and revenue on Pomegranate Industry in Major Regions.
The report analyses the entire production and supply chain of the industry and provides information as per different phases. Whereas, on the basis of sales, data related to sales volume, sales price, cost, sales income, and profit margin etc. of Pomegranate Market used in different fields, sold in different regions and by different companies is provided in this report.
Major Key Players are Analyzed in the Pomegranate Market Report such as:
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Market Segment by Regions, this Pomegranate market report splits Global into several key Regions, with sales (consumption), revenue, market share and growth rate of Pomegranate Market from 2011 to 2021 (forecast), like:
1. North America
2. China
3. Europe
4. Japan
5. Southeast Asia
6. India
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• Overview of Pomegranate Market
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• Research Findings and Conclusion of Pomegranate Industry
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Why Should We Defend Our Southern Heritage?
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text:
EURUSD had formed a cycle bottom at 1.3609
EURUSD had formed a cycle bottom at 1.3609 on 4-hour chart. Further rally is still possible in a couple of days, and target would be at 1.3950-1.4000 area. However, the rise is treated as consolidation of downtrend from 1.4246, another fall towards 1.3400 could be seen after consolidation, and a breakdown below 1.3609 could signal resumption of downtrend.


text:
You are viewing a fine 14K yellow gold Diamond cluster ring made in the 1970's. Every other row of Diamonds on the bottom are champagne colored stones. The ring measures 18mm or 3/4" long by 20mm. It is set with numerous round Diamonds weighing around 1.05 carats in total. The top Diamond is a 15 pointer. The ring is marked: 14K. It is a size 6 1/2 with a thick shank and can be re-sized. The ring weighs 9.1 grams.
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Re: [dogme] Not Formalism But "Contentism"
Expand Messages
- David,
....messages that pass......
Doesn't it always happen? I'd no longer written to say you hadn't really answered the
question I asked, when you answered it!
Thanks for that. Your answer to Dr. Evil and now this one have given me a much
clearer idea than I had before of some of the work you have done are are doing.
I'm not really as anti-theory etc. as I sound, you know. Partly I'm on the defensive after
a quarter of a century of subjection to the totally misconceived, tenth-rate so-called
"scientific approach" in Germany, and partly it's just the way I write - all those
monosyllables. :-))
Best wishes
Your message has been successfully submitted and would be delivered to recipients shortly.


text:
Category:Robert Heaton Rhodes Sr
|Authority control
Robert Heaton Rhodes Sr (1815–1884) was a New Zealand politician, who represented the Akaroa electorate from 1871 to 1874.
This category has only the following subcategory.
- ► Rhodes Memorial Home (5 F)
Media in category "Robert Heaton Rhodes Sr"
The following 3 files are in this category, out of 3 total.
- Elmwood, 1899.jpg 278 KB
- Robert Heaton Rhodes Sr.jpg 258 KB


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- High grade our Oklahoma business by focusing on the Central Oklahoma area where the liquids content is higher than in the Northern asset,
- Retain the majority of our Oklahoma assets providing significant upside for our shareholders associated with any recovery of natural gas and NGL prices to more historical levels,
- Best fit the risk-reward profile of a company our size in an area where we've had greater than 90 percent drilling success and have a multi-year inventory of drilling prospects, and
- Further improve the Company's balance sheet.
CALGARY, Alberta, Sept. 24, 2012 /PRNewswire/ - Equal Energy Ltd. ("Equal" or "the Company") (TSX: EQU) (NYSE: EQU) is pleased to announce that, through one of its wholly owned subsidiaries, it has closed an agreement with Atlas Resource Partners, L.P. ("Atlas") (NYSE: ARP) whereby Equal has sold its interest in its Northern Oklahoma assets located in Grant, Garfield and Alfalfa counties for total cash consideration of US$40 million, effective July 1 st 2012. The assets sold include production of 1,400 barrels of oil equivalent per day ("boe/day") ( July 2012 average; 73% natural gas, 25% NGL, 2% oil), related infrastructure and interests in approximately 8,550 acres of Mississippian lands. Don Klapko, President and Chief Executive Officer said, "The sale of the Northern Oklahoma asset is a key part of Equal's strategic direction which will:


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The Portuguese boss joins the principality club in place of Claudio Ranieri after a successful season with Sporting Lisbon, whom he led to second in the Primeira Liga
Jardim will begin taking training with his new players, who came second in Ligue 1 in 2013-14, on June 30.
"I am very happy to work at Monaco because it is a great club," the 39-year-old told Monaco TV. "I thought very hard about this project and I think we'll do a good job with the whole group.
"Monaco is a team with quality players. There are a lot of talented young players. I'll do everything [I can] to bring it forward.
"The first thing [I have to do] is to learn to speak French. I have been taking lessons for a few days. It is a question of respect for the fans, the people of the club and Ligue 1," he added.
Jardim joins Monaco after a successful season with Sporting Lisbon, during which he led them to second place in the Primeira Liga.


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Great commercial office space for small business in midtown Beaufort overlooking the Beaufort River/ Intracoastal Waterway. Adjacent to the shops and restaurants of the new Beaufort Town Center and Jean Ribaut Square. Near downtown Beaufort and government centers. Minutes to military bases and hospitals.
† Excluded Feature
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Information deemed reliable but not guaranteed.
Listing data provided courtesy of the Beaufort County Multiple Listing Service, Inc.
The information being provided is for consumers personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing.
Data is updated as of 5/25/2017.
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Default based on a 30-year fixed rate of 4.125% with 20% down. The estimated payment is offered for convenience and is not an offer of credit. Due to market fluctuations, interest rates are subject to change at any time and without notice. Your individual rate may vary. Rates may differ for FHA, VA or jumbo loans.


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Wednesday, October 5, 2011
Commercial Of The Day: Taxidermy
3 comments:
"The most life-like dead animals anywhere"--that's quite a selling point. That may be so, but I still don't want a dead animal hanging on my wall or staring down at me from above the mantle.ReplyDelete
- AnonymousOctober 5, 2011 at 1:24 PM
The LEAST lifelike animal there is good ol' Chuck himself. Seriously, has the guy had a serious stroke or what? None of his facial muscles move at all (except his mouth). That is seriously creepy!ReplyDelete
Prairie Girl.
My hometown newspaper ran an article about the local pharmacist who had a special climate-controlled barn built to house his 600+ taxidermy animals that he'd done himself. The middle school even took kids there on a field trip. No one seemed to feel this was a) beyond creepy and b) the basic plot setup of a future Criminal Minds episode. I used to babysit for this guy, too. Weird.ReplyDelete


text:
Heal JinxMature
You rush to her side and work your magic on her, watching carefully as within a second the wound has healed up and a barrier formed of shields circle her. Yet you have no more time as Blitzcrank's hand grabs you from the sides and yanks you into the duo. Fortune fires bullets into you, pain searing hot through your body. You can't give up now though, you must help Jinx.
You start to run away, doing anything to keep grip of the last strands of life yet they continue to chase you. There is only one thing to do to get to Jinx standing safely under the tower's watchful gaze. With an almighty push you flash yourself forward and are by her side in a blink of an eye. She looks at you and a single tear rolls down her cheek.
"I'm so sorry," she whispers, "They made me do it."
All you can manage is a nod as you wait for your astral blessing, the healing spell to warm up in your blood again and be ready to mend your bloody body. Only 15 seconds now but Jinx starts moving forward to attack and you know you must follow. The blood drips down your leg and is instantly soaked into the floor. The summoners never like to see their champions hurt.
Fortune, distracted with slaughtering the minions, turns to face Jinx running up. She mouths the word "no" but Jinx can't stop her feet in time as Blitzcrank's hand comes out the bush and yanks her in. You run as fast as you can to her and silence Fortune as she starts shooting. There is no hope though as Jinx is battered by both of them and you turn and flee before the same fate is dealt to you.
You pant under the tower, your astral blessing spell ready and use it on yourself. If only if it had been ready back when Jinx was being pummelled. You bet the summoner controlling her is upset but they have no clue what she is going through right now. If the actual murder hadn't been painful enough the pain of being brought back to life is torture. First you go to this entirely black room where magic is injected into your body and it runs through your veins. It's a searing hot burning sensation that goes all over your body until your system accepts it and starts healing. Then you are cut up into tiny particles and flown back to the base where you're put back together, ready to fight at their pleasure again. The time it takes to do this process depends on how late into the game you are at your death, the more time, the more exhausted you are and that makes your body more tired and resistant to the magic. You hate that place more than the warehouse.
Even though you have used your spell on yourself you are not fully healed and your minions are still pretty even on each side. Jinx has respawned and is half-way to the tower. She should be fine for a bit on her own you think. What do you wanna do?


text:
The Simpsons: Season 7 DVD
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The Simpsons: Season 7 DVD Product Description
Please note this is a region 2 DVD and will require a region 2 or region free DVD player in order to play.
The complete seventh season of hugely popular US comedy series. In this season you will find out who was behind the shooting of the nefarious Mr Burns. Episodes comprise: 'Who Shot Mr Burns? (Part 2)', 'Radioactive Man', 'Home Sweet Home-Dum-Diddly-Doodly', 'Bart Sells His Soul', 'Lisa The Vegetarian', 'Treehouse Of Horror VI', 'King-Size Homer', 'Mother Simpson', 'Sideshow Bob's Last Gleaming', 'The Simpsons' 138th Show Spectacular', 'Marge Be Not Proud', 'Team Homer', 'Two Bad Neighbours', 'Scenes From A Class Struggle In Springfield', 'Bart The Fink', 'Lisa The Iconoclast', 'Homer The Smithers', 'The Day The Violence Died', 'A Fish Called Selma', 'Bart On The Road', '22 Short Films About Springfield', 'Raging Abe Simpson And His Grumbling Grandson In 'The Curse of the Flying Hellfish'', 'Much Apu About Nothing', 'Homerpalooza' and 'Summer Of 4'2''.
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February 19, 2007
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Study Makes 11 Suggestions on the Ethical Responsibilities of Organizational Knowledge Regarding Antiretroviral Drug Innovation and Access
WASHINGTON -- A report released today by The George Washington University Law School's Creative and Innovative Economy Center (CIEC) outlines recommendations to ameliorate the growing AIDS crisis in Sub-Saharan Africa. The report was presented at a CIEC-hosted event in Geneva, Switzerland, for delegates attending the Development Agenda meetings at the World Intellectual Property Organization.
CIEC Director Michael P. Ryan and his team carried out field research in three African countries - Burkina Faso, Botswana, and Uganda - to study how access and treatment programs are being delivered. Antiretroviral (ARV) drugs can dramatically slow the progression of AIDS, but only if these drugs are made available at the medically appropriate dosages to HIV positive people. Twenty-six million people in Sub-Saharan Africa are HIV positive. The study notes that they and millions more will die of AIDS during the next 20 years absent massive, sustained, and organized intervention.
"AIDS activists declare that the drug companies must take immediate action to stem the crisis," observed Ryan. "But drug company leaders assert that African governments are responsible for the health of their peoples. This report asks who is responsible to do what and why regarding antiretroviral drug innovation and access in Africa."
"A social contract challenges wealthy countries and the drug companies to work more effectively with the governments of Sub-Saharan Africa," Ryan continued. "The burden of this crisis needs to be shared by all of the stakeholders." Ryan and his researchers identified 11 recommendations critical to defeating the pandemic:
1. Wealthy countries should invest in basic medical science in African countries;2. African governments should provide patent rights to encourage private drug development;3. ARV drug innovators should teach about their ARV therapies;4. ARV drug innovators should license production to developing country manufacturers;5. ARV drug innovators should transfer manufacturing technology;6. ARV drug innovators should donate and discount drugs by development level;7. ARV drug innovators should teach about drug distribution and logistics;8. Wealthy country governments should provide aid regarding ARV treatment;9. The World Health Organization should ensure that all ARV suppliers meet Good Manufacturing Practices;10. All African employers should try to provide ARV drug treatment; and11. African governments should coordinate public-private partnerships.
"There are no quick fixes to this health crisis," warned Ryan. "Each government must articulate comprehensive strategies and recruit partners with know-how and financial resources who are committed to sustaining programs over many years."
A copy of The AIDS Social Contract in Africa: On the Ethical Responsibilities of Organizational Knowledge Regarding Antiretroviral Drug Innovation and Access is available online at the CIEC?s Web site, www.law.gwu.edu/ciec.
The CIEC conducts research and educational activities that encourage developing nations to embrace creativity and innovation as tools to compete more effectively in the world economy. Stressing a multi-disciplinary approach, the center examines legal, commercial, institutional, and globalization issues pertaining to economic development. Programs will be conducted at The George Washington University in Washington, D.C., in Europe at the Munich Intellectual Property Law Center, and in developing countries around the world.
For more information about CIEC visit www.law.gwu.edu/ciec.For more news about GW, visit the GW News Center at www.gwnewscenter.org.
The George Washington University Office of University Relations,
Washington, D.C.


text:
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For the fourth year in a row, Mid-Park Inc. and Grayson County Schools are partnering for Manufacturing Week, which will serve to educate youth on manufacturing career options available to them.
Deputy Grayson County Judge Executive Tom Goff, Leitchfield Mayor William Thomason, Clarkson Mayor Bonnie Henderson, and Caneyville Mayor James Embry signed a proclamation declaring Manufacturing Week during the September Grayson County Chamber of Commerce membership luncheon on Thursday, Sept. 8.
Mid-Park President Greg Bernard said during the luncheon that Manufacturing Week serves as an opportunity to address common misperceptions about manufacturing by giving local manufacturers the opportunity to open their doors to the community and its youth.
Bernard said that at some point, vocational studies became "stigmatized," but it is important to expose young people to different careers and opportunities that can be just as fruitful as those attained by attending college.
This stigma has proven a difficult challenge for manufacturers to overcome as 84 percent of executives surveyed agree there is a talent shortage in United States manufacturing, according to Bernard.
Grayson County Manufacturing Week activities will include setting up a manufacturing display at Grayson County Middle School (GCMS) during the week of Sept. 19; speakers discussing careers in manufacturing at GCMS on Sept. 26 and 27; and manufacturing facility tours for middle schoolers and vocational students on Sept. 28.
Mid-Park has made itself a goal of promoting vocational studies in Grayson County, first in 2013 with a pledge of $25,000 to Grayson County Schools to establish a machining program and now in 2016 with another $25,000 pledge to Elizabethtown Community and Technical College for its expansion, Bernard said.
Also during the luncheon:
*It was announced that John Bouvier, of BeeHive Homes of Leitchfield, Trevor Ray, of Midway Pharmacy, and Misty Thomas, of Dan Powers GM Center, have joined the Chamber of Commerce Board of Directors.
Reach Matt Lasley at 270-259-9622, ext. 2015.


text:
Jian Ghomeshi Not Guilty On All Charges In Sexual Assault Trial
Jian Ghomeshi has been acquitted on all five charges against him, a judge ruled Thursday.
Justice William Horkins delivered his ruling in a provincial courtroom in Toronto's Old City Hall. Reading his decision to the court, the judge said the lack of a "smoking gun" or physical evidence presented a difficult bar for the Crown.
After the not guilty verdict was read to the court, Ghomeshi embraced his lawyer and turned around and hugged his mother who sat behind.
"The harsh reality is that once a witness has been shown to be deceptive and manipulative in giving their evidence, that witness can no longer expect the Court to consider them to be a trusted source of the truth," Horkins said.
Jian Ghomeshi leaves a Toronto courthouse on March 24, 2016 with his lawyer Marie Henein. (Photo:Christopher Katsarov/The Canadian Press via AP)
He noted "serious deficiencies" in evidence presented to him during trial, noting reasonable doubt exists in the case.
"I am forced to conclude that it is impossible for the Court to have sufficient faith in the reliability or sincerity of these complainants."
More coverage:
At the beginning of the reading of his decision, Horkins referenced the publicity that trailed the former "Q" host after he was fired from the CBC in 2014 as the "Ghomeshi scandal."
It took Horkins over a hour to read out his 26-page ruling, where he went through each witness' case laid against Ghomeshi.
In one portion, he outlined the possibility of collusion between two witnesses to see Ghomeshi "fucking decimated." A reaction that may have been fueled by the "animus" of anger reflective of "legitimate feelings of victims of abuse," he noted.
"I have a firm understanding that the reasonableness of reactive human behaviour in the dynamics of a relationship can be variable and unpredictable," said Horkins.
He noted some details didn't line up to what witness' told police and what they said in court, adding pieces of evidence were "tainted by outright deception."
In cases with criminal charges, Horkins stressed presumption of innocence for the accused isn't something that should be immediately waived.
"However, the twists and turns of the complainants' evidence in this — 24 — trial, illustrate the need to be vigilant in avoiding the equally dangerous false assumption that sexual assault complainants are always truthful."
Protesters stand outside court in Toronto on March 24, 2016 awaiting a verdict on the sexual assault trial of former CBC personality Jian Ghomeshi. (Photo: Frank Gunn/The Canadian Press)
Testing reliability of alleged victims of sexual assault
Trigger warning: This article contains information about violence which may be triggering to survivors.
During the trial, Ghomeshi's high-profile defence lawyer Marie Henein uncovered alleged inconsistencies in the testimonies of three complainants who came forward to police.
She brought forward surprise documents, including emails sent from the witnesses, before the judge.
The defence strategy focused on memory, credibility and reliability of the witnesses, and the actions of the complainants after the alleged sexual assaults took place between 2002 and 2003.
"It suggests a degree of carelessness with the truth that diminishes the general reliability of the witness"
— Justice William Horkins
Horkins told the courtroom one witness' narrative — actress Lucy DeCourtere — shifted during trial, saying she told the media one story and the court another.
He referenced an email she sent Ghomeshi on July 5, 2003, a day after he allegedly choked her:
Getting to know you is literally changing my mind, in a good way. You challenge me and point to stuff that has not been pulled out in a very long time. I can tell you about that sometime and everything about our friendship so far will make sense. You kicked my ass last night and that makes me want to fuck your brains out, tonight.
After the email was presented in court, the "Trailer Park Boys" actress told the Toronto Star it doesn't derail the fact she was choked and slapped "without consent."
But with "not a trace of animosity, regret, or offence taken," the judge disagreed.
"It suggests a degree of carelessness with the truth that diminishes the general reliability of the witness," Horkins wrote in his ruling.
Calling details of the alleged incidents of "historical complaints," the judge acknowledged each complainant expressed "very valid" reasons for not coming forward to police until 2014.
A copy of a photograph of Lucy DeCoutere released by the court during the trial of Jian Ghomeshi on Feb. 5, 2016. (Photo: Handout)
The names of the two other alleged victims are under publication ban.
CBC: Firing Ghomeshi was right decision
Ghomeshi faced five charges at the trial's start: four counts of sexual assault and one count of overcoming resistance by choking. He opted to be tried by a judge, not by jury.
He pleaded not guilty to all five charges against him in October.
Workers scrape a wall which had a publicity photo of former CBC radio host Jian Ghomeshi in the broadcasting corporation's Toronto offices on October 27, 2014. (Photo: Chris Young/The Canadian Press)
A spokesperson representing Ghomeshi's former employer, the CBC, commented that the decision Thursday is unrelated to the public broadcaster's decision to fire him in 2014.
"Based on the evidence that came to our attention, Mr. Ghomeshi's actions were not in line with the values of the public broadcaster nor our employee code of conduct," said Chuck Thompson in a written statement.
"We stand by our decision."
'No right way to be a victim of sexual assault'
In an October 2014 internal memo to staff, the CBC explained the dismissal of one of its biggest celebrities by saying managers had seen "graphic evidence" the former radio host had "caused physical injury to a woman."
After he was fired, Ghomeshi posted a long and personal post to Facebook in his defence about his dismissal and "a campaign of false allegations pursued by a jilted ex-girlfriend and a freelance writer."
That Facebook post, which was eventually deleted after being widely shared, which closed the door on reasonable doubt and opened the opportunity for the Toronto Star to publish its original bombshell story.
"And you wonder why women do not come forward after abuse."
— Lisa MacLeod, Ontario MPP
Ghomeshi's sudden and dramatic fall triggered a national conversation about how the public and justice system treat survivors of sexual assault.
Across the country, news of his acquittal prompted supporters and victims advocates to band behind the hashtag #IBelieveSurvivors — encouraging women to not be discouraged by the verdict and to continue reporting sexual assault allegations to police.
Tragically, our systems continue to put survivors of sexual assault on trial while their abusers and rapists go free. #IBelieveSurvivors
— Janet Mock (@janetmock) March 24, 2016
Don't let the #Ghomeshi ruling keep you from naming your abuser. We need to keep fighting for all survivors. #IBelieveSurvivors
— Margaret Cho (@margaretcho) March 24, 2016
And you wonder why women do not come forward after abuse. #IBelieveSurvivors
— Lisa MacLeod (@MacLeodLisa) March 24, 2016
There is no right way to be a victim of sexual assault.
There is no right way to be a victim of sexual assault.#IBelieveSurvivors
— Rakhee Sapra (@RakheeSapra) March 24, 2016
Horkins' ruling doesn't mark the end for Ghomeshi's interaction with the criminal justice system.
The former CBC Radio host faces a sexual assault charge filed by another complainant stemming from an incident that allegedly happened in the workplace.
A separate trial will hear that case in June.
Also on HuffPost:
A former York University student, Kerry Eady, told the Toronto Star she was at an informal meeting of about 25 students in the fall of 1988. Residence advisers told the group that a couple of female students had said Ghomeshi hit them. One said she was choked in a stairwell, Eady recalled. "There was nothing official, because these girls were talking to [the advisers] confidentially, to get advice on what to do. They told them to report it but none of them wanted to," Eady told The Star. Source: Toronto Star
Jim Hounslow attended served as communications coordinator for York's student federation while Ghomeshi was president. Hounslow told the Star that he and Ghomeshi were waiting for an elevator after a student meeting during the 1990-91 school year when Ghomeshi suddenly reached over and grabbed his genitals through his jeans. "I was completely shocked and I reacted," Hounslow told The Star. He grabbed Ghomeshi's arm, pushed him against the elevator doors and told him to never do that again. Source: Toronto Star
Moxy Fruvous fan Sally Block was a 16-year-old living in upstate New York in 1999, and she was surprised when Ghomeshi responded to her email. That initiated a multi-year correspondence that grew to include phone calls. She also met up with Ghomeshi after concerts where Block says he sometimes got "handsy." After she stopped hearing from Ghomeshi in 2002, Block broke into his email by guessing his password. Ghomeshi threatened to press charges, but backed down after Block's father learned of his history of correspondence with his then-underage daughter. Source: The Globe and Mail
Author and lawyer Reva Seth says that she went on a few casual dates with Ghomeshi in 2002. In a blog for HuffPost, she wrote that she was at his home one evening when suddenly "he became a different person." Ghomeshi put his hands around her throat and "violently digitally penetrat[ed]" her. Source: Huffington Post Canada
An anonymous woman says she met Ghomeshi after a taping of his CBC show "Play" in 2002. She says he yanked her hair hard at one point in the evening and asked her if she liked it "rough." She met him again two weeks later, and went back to his home. She says that he grabbed her hair without her consent and gave her three sharp punches to the head. "I was crying. Just crying. He stood there looking at me and said, 'You should leave,' " she told the Star. The same woman was also interviewed by CBC's "As It Happens." Source: "As It Happens" and Toronto Star
The former "Trailer Park Boys" actress says that Ghomeshi, without warning or consent, choked her to the point she could not breathe and then slapped her hard three times on the side of her head. She described the same events again in interviews with the CBC. Source: Toronto Star
A woman sent Ghomeshi a letter asking him to manage her music career, and he called her back. When they met in person, she says Ghomeshi groped her, tried to kiss her and "relentlessly tried to put his hands on and near my inner thighs, crotch and rear, both inside and out of my pants," the Star reported. Source: Toronto Star
An anonymous woman alleges that Ghomeshi attacked her after they went for a walk in a Toronto park following an event. She had been on a few dates with him before, but they had never been intimate. She told The Star he choked, smothered, bit and groped her on a park bench. Source: Toronto Star
A 22-year-old woman met Ghomeshi at a Christmas party. The woman, who worked at the CBC, went out for a drink with him and on the way back to his car, he started choking her with no warning. "I said, 'What the f---.' I told him I have three brothers," she told the Star. She says Ghomeshi replied "your loss." She says he drove away and then called her to say he was under a lot of stress and driving fast — "if I crash it will be your fault." Source: Toronto Star
The former "Q" producer's allegations were initially anonymous, but Borel identified herself in a column about Ghomeshi's workplace harassment for The Guardian on Dec. 2. She alleges that Ghomeshi told her after a story meeting in 2007 "I want to hate f--- you." After that, he gave her uninvited back massages at her desk and more harassment followed over the next three years, until she left the CBC. "A year into my time on the job, he grabbed my rear end and claimed he couldn't control himself because of my skirt," she wrote for the Guardian. "He once grabbed my waist from behind – in front of our fellow colleague, at the office – and proceeded to repeatedly thrust his crotch into my backside. "There was emotional abuse, too: gaslighting and psychological games that undermined my intelligence, security and sense of self. Sometimes that hit harder than the physical trespassing." Source: The Guardian
A woman told The Toronto Star that Ghomeshi did ask her if she "was into choking," telling her "it would heighten the experience of sex." The woman says she told Ghomeshi she wasn't into choking. She also recalls how he hit her and called her "a slut." Source: Toronto Star
A 23-year-old woman applied for a position as Ghomeshi's assistant, but withdrew her application after she met with him and he was critical of her appearance and mispronouncing his name. "I genuinely felt so bad about it at the time. I look back on this now as the tactic of a manipulator with a mandate," she told the Star. Ghomeshi continued to contact her and they began dating. She says he hit her on her head and face with an open hand. "I pushed him back once and he grabbed my wrists and said he did not like that," she told the Star. Source: Toronto Star
A female fan met Ghomeshi at a book signing in a small city in Eastern Canada in 2012. They later corresponded by text, and many of the messages were violent, she said. They eventually met in person, and he allegedly shoved her against a wall, choked, and hit her on the head. Source: Toronto Star
The Toronto Star reported an anonymous woman in her mid-20s says she met Ghomeshi at a book signing in a small city in Eastern Canada. He invited her to visit him in Toronto, where she says he threw her against the wall. She said sex with the host included belts, choking and hitting that left her bruised. Ghomeshi allegedly found the bruises "sexy." Source: Toronto Star
A Western University graduate told the Star about her experience with Ghomeshi on condition of anonymity. She said that as a student, Ghomeshi touched her inappropriately after a taping of "Q." She went to the taping in Dec. 2012 in the hopes of landing a job on the show. She alleges that after the show, Ghomeshi approached her from behind and pressed his body against her. He later asked her out for a "non-work related drink" but she said she didn't want to date and was only interested in discussing a job. He responded that he didn't want to be a "conduit to a job." Source: Toronto Star
A CBC producer in her mid-20s went out for drinks with Ghomeshi following a book signing in Montreal. She told him her dream job was to work for "Q." He rubbed her legs in a pub, telling her that "touching helps" his anxiety. She said she went to Ghomeshi's hotel room, where he threw her against a wall and fondled her. She said she performed oral sex on him "just to get out of there." Source: Toronto Star
A woman, who was a student at the time, said she had a five-month relationship with Ghomeshi. She says he tried to smother her by covering her nose and mouth with his hands. She told the Toronto Star she used her cellphone to call a friend from Ghomeshi's bathroom, who told her to "get out of there." She left and broke off the relationship with him. Source: Toronto Star
A Toronto woman shared a hotel elevator with Ghomeshi while he was in London. She told Ghomeshi her name, and he tracked her email down and contacted her for a drink. "After a few drinks we went back to his room where he proceeded to literally throw me on his bed, no buildup, no conversation, and started biting, pulling my hair and biting me all over," she told the Star. Source: Toronto Star
Although Carla Ciccone's blog for XOjane never identifies the man in the story is Ghomeshi, it is widely assumed to be — Ghomeshi himself has not denied going on a date with Ciccone. The blog details a date with a Canadian media personality, which quickly turned sour when he touched her without consent — on multiple occasions. After the Ghomeshi story broke in Oct. 2014, Ciccone tweeted this about the controversy. Source: XOjane
An anonymous woman told The Star that she had gone out on a few dates with Ghomeshi in 2013 but they never had sex. Weeks later, Ghomeshi invited her over to his new house in Toronto, telling her he "didn't buy this big house to throw parties, but to raise a family." He then kissed her, and then suddenly pulled her hair and went on to hit her, bit her and called her a degrading term. Source: Toronto Star
A Twitter account with the handle @BigEarsTeddy began posting allegations of sexual violence against Ghomeshi in April 2014. The identity of the Twitter user is not known. Source: Twitter
Ghomeshi hired Marie Heinen, a criminal lawyer who previously defended former attorney general Michael Bryant, reports The Toronto Star. In a Toronto Life profile, Heinen says she is "not conflicted about being a strong feminist and what I do in court."
The Toronto Star reports that a woman who has never spoken to the media did file a police complaint. Her allegations partly led to the five abuse-related charges laid against Ghomeshi on Nov. 26. Source: Toronto Star
A woman who bumped into Ghomeshi at the CBC building in Toronto told the Star that she gave him her phone number. They went on a few dates and he sexually assaulted her at his home on several occasions. Source: Toronto Star
Two of the seven counts of sexual assault against Jian Ghomeshi were dropped due to "no reasonable prospect of conviction."
Ghomeshi pleaded "not guilty" to five charges, included four counts of sexual assault and one count of overcoming resistance by choking. The court date is set for Feb. 1, 2016.
Feb. 1, 2016: Ghomeshi's judge-alone trial gets underway in Toronto. Justice William Horkins says he'll deliver the verdict on March 24.
On March 24, 2016, Jian Ghomeshi was acquitted on all five charges against him, a judge ruled. Justice William Horkins delivered his ruling in a provincial courtroom in Toronto's Old City Hall. Reading his decision to the court, the judge said the lack of a "smoking gun" or physical evidence presented a difficult bar for the Crown.


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China vowed yesterday to crack down on unrest in Tibetan areas and accused overseas activist groups and the Dalai Lama of orchestrating the recent violence.
Foreign Ministry spokesman Liu Weimin said clashes between Tibetans and security forces in Sichuan province last month were the work of criminals and were instigated by overseas groups campaigning for Tibetan independence.
"We believe that this is a case of a handful of criminals illegally gathering and smashing and looting," Mr Liu said during a briefing.
He added that the "surprising" promptness with which overseas Tibetan activist groups reported the unrest "showed that they have colluded and premeditated the incidents". Mr Liu also accused the Dalai Lama, the Tibetans' spiritual leader, of preaching that actions were more powerful than prayer.
"The Chinese government will resolutely crack down on any attempt to incite violence or to disrupt national unity and integrity," Mr Liu said.
His remarks came as the Tibetan government-in-exile in Dharmsala, India, called for vigils, which are due to be held today.
More than a dozen monks and nuns have set themselves on fire in ethnic Tibetan areas of Sichuan over the past year. Most have chanted for Tibetan freedom and the return of the Dalai Lama, who fled to India after a failed uprising in 1959.
- More about:
- Department Of Foreign Affairs
- Sichuan Province
- Tibet


text:
Family of Woman Found in SF Hospital Stairwell Sues
The family of a woman who was found dead in a hospital stairwell is filing a claim against the City and County of San Francisco, claiming negligence.
An attorney for Lynne Spalding's family said the 57-year-old woman's death at San Francisco General Hospital last fall came as a result of "reckless neglect of her care" by hospital staffers and the sheriff's department, which provides security at the facility.
Spalding was reported missing from her room several days after being admitted for treatment of an infection on Sept. 19.
It wasn't until Oct. 8, when her body was discovered.
The claim seeks damages from the city.
READ MORE: SF Hospital: Body Found in Stairwell that of Missing Patient


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ibis Munich Garching HotelEconomy hotel for business and leisure
The Ibis München Garching hotel is situated in the Garching-Hochbrück business district, where you can enjoy the peace and quiet of nature while still being close to the city. All of the 105 comfortable, air-conditioned rooms have Internet access, as do the hotel's public areas. You can also surf for free using the iPoint in the lobby. Our reception and gastronomic service is available 24/7 and we serve a generous breakfast buffet between 4am and 12 noon. Motorcycle parking is provided free of charge.
Your ibis München Garching has just been renovated - we've given our public areas, restaurant and breakfast room a great makeover for our guests.
This hotel participates to Planet 21 and is acting in favour of Positive Hospitality.
Services in the hotel and nearby
Online check-in
Online check-in: Your arrival will be carefully prepared by our team
Fast check-out: Simply return your key
WIFI/Internet Access
- WIFI at the hotel
- WIFI in the communal areas
- WIFI in your room
- WIFI access
- Private indoor parking (Paying)
- Private outdoor parking
- Public outdoor parking
- Bus/coach parking area
Accessibility for people with reduced mobility
- Reduced mobility rooms
- Wheelchair access
- Reduced mobility facilities
- Accessible bathroom
- Pet allowed 8 EUR
- Air Conditioning
Services and practical information
Online check-in
Online check-in: Your arrival will be carefully prepared by our team
Fast check-out: Simply return your key
WIFI/Internet Access
- WIFI at the hotel
- WIFI in the communal areas
- WIFI in your room
- WIFI access
- Private indoor parking (Paying)
- Private outdoor parking
- Public outdoor parking
- Bus/coach parking area
Accessibility for people with reduced mobility
- Reduced mobility rooms
- Wheelchair access
- Reduced mobility facilities
- Accessible bathroom
- Pet allowed 8 EUR
- Air Conditioning
Languages spoken at the hotel
- German
- French
- Reception open 24 hours a day
- Shoe shine machine
- Currency exchange
Leisure and activities
- Jogging track
- 1 restaurant(s)
- 1 bar(s)
Business services
- Self service business centre
Hotel location
Daimlerstrasse 5
Tel: (+49)89/323840
Fax: (+49)89/32384100
- F.J.STRAUSS AIRPORT At 20km / 12.43 miles
- GARCHING SUED At 1km / 0.62 miles
- MUNICH/MUENCHEN At 20km / 12.43 miles
Currently, close to our hotel
Our rooms
We wish you a pleasant stay: All 105 rooms are air conditioned, soundproofed, and feature high-quality furnishings with the ultimate Sweet Bed by ibis sleeping comfort. With free WIFI and TV with a wide range of Sky channels.
Room with 1 double bed (63 x 79 inches [160 x 200 cm]), new sleep-easy concept
Give yourself plenty of time and space to sleep: There's ample room in the spacious Sweet Bed by ibis double bed, even for 2. The room is air-conditioned and features a functional desk, 24-hour WIFI and a flat-screen TV.
Room with twin beds, new sleep-easy concept
The air-conditioned room features Sweet Bed by ibis twin beds for maximum sleeping comfort. A desk and WIFI are available for work. If you would prefer to watch television, you can settle down in front of the flat-screen TV.
MyRoom by Michaela: Relax Zone
Do you like being in nature? Does the greenery help you to relax? Then a visit to Michaela's Relax Zone will feel like a visit to the moors.
- 105 rooms
- 84 non-smoking rooms
- 3 rooms for people with reduced mobility
- 5 connecting rooms
- Extra bed in room for child
- Crib on request
- Air conditioning
- Data port in room
- Emergency exit map
- Air Cooling System
- Bathroom doors 32 inches wide
- Telephone
- Radio in room
- Opening windows
- Remote control TV
- WIFI in your room
- Direct dial telephone
- Work desk in all rooms
- DDI - Direct Dialing In
- Satellite/cable colour TV
- Manual temperature control
- Crib on request
- High speed transmission line
- RJ 11 outlet
- Shower
- Toilets
- Hair dryer in bathroom
- Hair dryer at reception
- Keycard-operated door locks
- Deadbolts and/or locks in rooms
- Emergency info in rooms
- Message alert
- Operator wake up call
- Automatic wake up call
Our restaurants and bars
- Mon
- Tue
- Wed
- Thu
- Fri
- Sat
- Sun
IBIS KITCHENType of cuisine: Regional
Do you love concentrating on the important things in life? Then the ibis kitchen is perfect for you. Come and enjoy classic German cuisine in a simple atmosphere where you will feel comfortable immediately.
- Mon
- Tue
- Wed
- Thu
- Fri
- Sat
- Sun
- Mon
- Tue
- Wed
- Thu
- Fri
- Sat
- Sun
Our bar is always there for you: Every day, we serve round-the-clock beverages and refreshments, hot drinks and tasty food. Crispy paninis are available in five delicious versions, and can also be taken away! Live sporting events are shown on Sky.
Our customer reviews for ibis Munich Garching
Reviews collected from customers who booked on AccorHotels.com
The staff were very friendly, the location was convenient (train stop adjacent to the hotel goes directly to the city centre), the room was extremely comfortable and the prices were reasonable. I thoroughly recommend it!!!!
Dear Sir, thank you so much for this very fine and good remarks, that does the team doing our business on a right wayand makes us happy when our clients feel well. Have a good summertime and see you soon in an Ibis Hotel, kind regards Ursula Grießhaber
Breakfast was very good, with ubahn to centre very good connection, Very nice personnel, good quality of the bed. Good atmosphere at hotel. I was very happy with my wife to stay at this hotel and recommend it to all the others. Very good price for parking!!!
looks like a newly built or recently renewed. nice place, near U-bahn to the munich city. quiet calm place. very clean. good service. suggest to take room with windows to the U-bahn, because opposite are storage house and start work early and it gets very noisy even with closed vacum windows. To sum up good value for the money, good location
I was given a smoking and twin bedroom despite my preference (non-smoking / double bed). Staff forgot to give my the internet login details eventhough they noticed I booked as business -package option. Check-in time was pretty long. Not the best stayI had in Munich...
Stayed here for a night and the hotel is very nice. Rooms are standard Ibis - clean, comfortable - and is located very close to the U-Bahn that takes you to the Marienplatz in Munich. Nothing bad to say, so highly recommended.
When making a booking at this hotel:
- I earn Status & Rewards points
- I do not earn points
- I can easily convert my HuaZhu Rewards points into Le Club AccorHotels points (and vice versa)
- I can use my Rewards points
- I cannot use my points
- I benefit from {{fidelityCard}} advantages
- I benefit from advantages
- I do not benefit from advantages
- I can take advantage of the Members' rate
- I cannot take advantage of the Members' rate


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|Ballast Point Pumpkin Down
|Ithaca Excelsior! Nineteen
|Ithaca Mudder Earth
|Ithaca LAWNCH
|Ithaca Doppler Effect
|Ithaca Smokin Porter
|Ithaca Black Magic Berry
|Ithaca SaScotch
|Ithaca The Creeker
|Ithaca Rube Goseberg
|Ithaca Jareth the Hoplin King (Brett)
|Ithaca 5 OClock Doppelbock
|Ithaca Excelsior! Eighteen
jeremytoni (175) Swedesboro, New Jersey | October 12, 2011
Decent little place to stop by if you are in Ithaca. They will pour you a free sample of all the beers that they have available.
Boudicca (1227) London, England | June 10, 2015
When we last visited the Ithaca Beer Company their tap room was in a small, functional building just off the main road. This time we arrived three weeks after theyd opened their new place, quite close by and easy enough to find down a track known appropriately enough as Beer Drive. Theyve put a lot of thought into the design. Theres a nice curved bar with bottle tops embedded into the surface underneath, taps (all their own beer) set around a brushed stainless steel cylinder and a nice garden overlooking fields and the hills beyond. They have several acres where theyre growing hops and vegetables for use in the kitchen alongside produce from other small local producers. As an aside, when we visited the old place we met a local wine producer from the Six Mile Creek winery and distillery up the road. They dont have beer there but its a lovely place to go for a wine tasting and their gin is some of the best Ive ever tasted. (Last visited 2 November 2012).
Drake (781) Milwaukee, Wisconsin | February 21, 2015
Farmhouse vibe, bright and open. Long bar, lots of tables and retail towards the front. Food looked and smelled good but I did not partake. Friendly, helpful staff. Good prices. 5 tap room only beers on offer as well as a guest cider, and several regular beers. Beer quality was top notch. Worth the trek.
jb (162) Rochester, New York | October 25, 2014| Updated November 12, 2015
Great atmosphere especially when outdoor seating available. Ridiculously bad service at the bar but it was busy. Great service at our table and knowledgeable on the beer. Great food pub selections and 3 small batch beers available only there.
jtclockwork (488) , New Jersey | June 26, 2014
Small brewery. A few taps and bottles for sale. A bit disorganized at the release I went to. People walked out with entire cases of beer while dozens got shut out with nothing.
UDBeernut (348) Saint Johns, Florida | April 18, 2014
went for the Luminous release.,Big well lit open area. The bacon burger was tasty. would go back.
machfive55 (45) Columbia, Pennsylvania | October 28, 2013
This was a pretty nice place, very busy on a Saturday afternoon. They have a good number of beers on tap at one time, probably more than 6 as listed. The food was fairly good, typical brewpub style food. One of the better places in the area.
solidfunk (380) Washington D.C., Washington DC | September 12, 2013
A nice brewpub with a beautiful setting on a farm outside Ithaca. A great place to sample their beers, and they often have a lot of specials and one-offs on tap through their 5-barrel series. Service was super friendly. Beautiful patio.
Brewbie (18) New York | March 9, 2013
Stopped at the new expansion and relocation out behind the old place. Nice and spacious. Open seating area and a nice bar with seating also. Pub food, samplers and pints. Outside there are picnic tables and fire pits. Quite a dfference from the old place. Still have merchandise, bottle/case/growler sales. They also have a root beer and a ginger beer. Food was fine, the service quick and friendly, looking forward to stopping back again when the weather warms and enjoy a pint outside and take the brewery tour.
Ron (174) Rochester, New York | October 14, 2012| Updated February 23, 2014
From Brew trip 2011. Got there shortly sfter opening about 11am on a sat. (Delayed by town cops giving me a speeding ticket by Wegmans) My group of 4 had the lone operators sole attention for a while as he poured us a free sample of the 8 beers he had on tap. Very good. We each purchased some beer to take with us. (Not much of a selection that day) 1 friend was glad to purchase some good rootbeer that day. 2013 We stopped there in late January with the Finger Lakes Beer Trail winter Passport. The new Brewpub looked great. Good food and beer. Had great time. Got beer to go.
AleWife725 (66) Albany, New York | July 5, 2012
October 2011. A hidden gem. Looks like the front of a convenience store, and the ambiance is that of one too... except for the big wooden bar with all the taps off to the side. We tried 6 beers for free, with generous pours of each. Their beer tasted wonderfully fresh and the girl pouring was good to chat with. They sell beer lip balm there- I love their Flower Power version! A worthwhile stop, I will definitely be back.


text:
Annette Kutilek
Archived Record Omaha, NE — Previous Treasurer for Crocker Claim Service of Omaha Inc
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Identification and profiling of conserved and novel microRNAs from Chinese Qinchuan bovine longissimus thoracis
Non-human and non-rodent vertebrate genomics
MicroRNAs (miRNAs) are a family of ~22 nucleotide small RNA molecules that regulate gene expression by fully or partially binding to their complementary sequences. Recently, a large number of miRNAs and their expression patterns have been identified in various species. However, to date no miRNAs have been reported to modulate muscle development in beef cattle.
Total RNAs from the Chinese Qinchuan bovine longissimus thoracis at fetal and adult stages were used to construct small RNA libraries for Solexa SBS technology sequencing. A total of 15,454,182 clean reads were obtained from the fetal bovine library and 13,558,164 clean reads from the adult bovine library. In total, 521 miRNAs including 104 novel miRNA candidates were identified. Furthermore, the nucleotide bias, base edit and family of the known miRNAs were also analyzed. Based on stem-loop qPCR, 25 high-read miRNAs were detected, and the results showed that bta-miRNA-206, miRNA-1, miRNA-133, miRNAn12, and miRNAn17 were highly expressed in muscle-related tissue or organs, suggesting that these miRNAs may play a role in the development of bovine muscle tissues.
This study confirmed the authenticity of 417 known miRNAs, discovered 104 novel miRNAs in bos taurus, and identified five muscle-specific miRNAs. The identification of novel miRNAs significantly expanded the repertoire of bovine miRNAs and could contribute to further studies on the muscle development of cattle.
MicroRNAs (miRNAs) are a new class of single-stranded endogenous non-coding small RNA molecules (~22 nucleotides) that bind primarily to the 3′UTR of target mRNAs to repress their translation and accelerate their decay
Currently 19,724 mature miRNAs have been discovered from 153 species and deposited in the publicly available miRNA database miRBase (Release 17.0, April 2011) (
Given the emerging roles of miRNAs in development, identifying the differentially expressed miRNAs is an important first step to investigating the function of miRNAs in the course of bovine growth and development. In this study, we have constructed two small RNA cDNA libraries from Chinese Qinchuan bovine longissimus thoracis at fetal and adult stages. By high throughput sequencing of the small RNA libraries and subsequent bioinformatic analysis, miRNAs in the longissimus thoracis were identified. In addition, the expression patterns of the high-read miRNAs from differential tissues (muscle, heart, liver, lung, kidney, brain, intestine, fat, and spleen) at multiple developmental stages of bovine muscle tissues (fetal, calf, and adult) were evaluated. Elucidation of the expression patterns of different miRNAs among different tissues will contribute to understanding the roles of miRNAs in gene expression regulatory networks for particular biological functions in livestock species.
Results and discussion
Tissue collection and high-throughput sequencing of small RNAs
Skeletal muscle is composed of myofibers, intramuscular adipocytes and connective tissue. Myofibers are the structural units of skeletal muscle
To identify the small RNAs involved in bovine muscle proliferation and differentiation, total RNAs from bovine longissimus thoracis at fetal and adult stages were used to construct small RNA libraries. We obtained 15,454,182 clean reads from the fetal bovine library and 13,558,164 clean reads from the adult bovine library after deleting some contaminant reads (Table
Figure 1
Length distribution of small RNAs in the fetal bovine (black) and adult bovine (gray) libraries
Length distribution of small RNAs in the fetal bovine (black) and adult bovine (gray) libraries.
Identification of conserved bovine miRNAs
There are currently 662 bta-miRNA precursors and 674 mature bta-miRNAs containing 577 bta-miRNAs, 42 bta-miRNA*, 28 bta-miRNA-5p and 27 bta-miRNA-3p in miRBase. To identify conserved miRNAs in the skeletal muscle tissue of the Qinchuan bovine, the small RNAs with a length of 18–23 nucleotides were Blastn searched against the miRBase 17.0 (miRBase release version V17.0, April 2011). 5,130 unique sequences (9,512,874 reads) were annotated as miRNA candidates in the fetal bovine library as well as 2,508 unique sequences (11,751,013 reads) in the adult bovine library, while the rest were unannotated. The miRNA candidates were then clustered into 407 and 289 categories corresponding to 406 and 299 independent genomic loci in the two libraries according to sequence similarity (Table
Additional file 1
Bovine conserved miRNAs.
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Additional file 2
The annotated miRNA and its miRNA* in the fetal bovine library.
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The expression of known miRNAs in the two samples was demonstrated by plotting Log2-ratio and Scatter Plot (Figure
Additional file 3
The known miRNAs expression profiles between two libraries.
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Additional file 4
Nucleotide bias at each position of sRNA tags. Note: miRNA nucleotide bias at each position of adult bovine muscle tissue (A) and fetal bovine muscle tissue (B).
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Figure 2
The differential expression of bovine conserved miRNAs between fetal bovine and adult bovine muscle tissue are shown
The differential expression of bovine conserved miRNAs between fetal bovine and adult bovine muscle tissue are shown. Note: Expression level (1): Expression level of adult bovine muscle tissue; Expression level (2): Expression level of fetal bovine muscle tissue. Each point in the figure represents a miRNA. Red points represent miRNAs with a fold change > 2, blue points represent miRNAs with 1/2 < fold change ≤ 2, green points represent miRNAs with fold change ≤ 1/2.
Position 2–8 of a mature miRNA is called the seed region, which is highly conserved. The target of a miRNA might be different with the change of nucleotides in this region. In our analysis pipeline, miRNAs that might have base edit can be detected by aligning unannotated sRNA tags with mature miRNAs from miRBase17, allowing one mismatch at a certain position. The results showed that approximately 23.41% in the adult bovine library and 29.06% in the fetal bovine library of the identified miRNA sequences were found to have mismatches that were caused by post-transcriptional modification, and/or RT-PCR, and sequencing errors (Additional files
Additional file 5
Summary of base edit in adult bovine library.
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Additional file 6
Summary of base edit in fetal bovine library.
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Additional file 7
End variants of the fetal bovine library identified in this study.
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To date, 2,042, 1,281, 791, 289, 360, 103, 247, and 306 conserved miRNAs were identified in human, mouse, chicken, dog, horse, sheep, zebrafish and wild boar, respectively
Additional file 8
Family analyses of known miRNAs.
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Identification of novel bovine miRNAs
The characteristic hairpin structure of a miRNA precursor can be used to predict novel miRNA. Prediction software Mireap
Additional file 9
Novel miRNAs identified in this study.
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Validation of related bovine miRNAs
The miRNA expression patterns in different tissues have been investigated in cattle
Additional file 10
The expression of miRNAs in bovine tissues and organs were detected by RT-qPCR. Note: FM: fetal bovine muscle tissue; CM: calf bovine muscle tissue; AM: adult bovine muscle tissue.
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Figure 3
The expression of miRNAs in bovine muscle-related tissue or organs were detected by RT-qPCR
The expression of miRNAs in bovine muscle-related tissue or organs were detected by RT-qPCR. Note: FM: fetal bovine muscle tissue; CM: calf bovine muscle tissue; AM: adult bovine muscle tissue.
To further explore the muscle-specific miRNAs involved in the muscle development of the cattle, we performed quantitative analysis of the miRNAs in fetal (day 90 bovine embryos), calf (3-day-old) and adult (2-year-old) bovine longissimus thoracis. The results showed that expression of bta-miRNA-133 and miRn12 was increased in the muscle tissues from day 90 bovine embryos to 2-year-olds, respectively. However, the expression levels of bta-miRNA-206, miRNA-1, and miRn17 did not change between day 90 bovine embryo and 2-year muscle tissues, but significantly increased in the calf muscle tissue (Figure
We have identified 417 known miRNAs and 104 novel miRNAs in longissimus thoracis from fetal and adult Qinchuan bovine using deep sequencing technologies. This study expands the repertoire of bovine miRNAs and could initiate further study in the muscle development of cattle. In addition, the miRNA expression patterns among nine tissues in beef cattle showed that most miRNAs are ubiquitously expressed, suggesting that these miRNAs may play a role in a broad range of biological processes in various tissues. However, we have also identified some muscle-specific miRNAs, suggesting that these miRNAs are likely to play a role in the development of bovine muscle tissues and could be potential molecular markers for genetics and breeding.
Ethics statement
All animals in this study were maintained according to the No. 5 proclamation of the Ministry of Agriculture, P. R. China. Sample collection was approved by the Animal Care Commission of the College of Animal Science and Technology, Northwest A&F University. Bovine embryos of slaughtered cows were collected from Tumen abattoir, a local slaughterhouse of Xi'An, P.R. China. A newborn Qinchuan calf and adult Qinchuan cattle were obtained from Meixian Qinbao Co., Ltd.
Tissue collection and high-throughput sequencing
Day 90 (d90) bovine embryos (gestation period 280 days) were collected into sterile physiological saline immediately after removal from the reproductive tract of slaughtered cows at a local abattoir. Fetal age was estimated based on crown-rump length
Small RNA sequence analysis
After clearing away the 3′ adaptor sequence, removal of redundancy and reads smaller than 18nt, the clean reads were screened against and mapped to the latest bovine genome assembly
MicroRNA expression analysis
Comparison of the known miRNA expression between two samples was conducted to find out the differentially expressed miRNAs. The expression of miRNA was shown in two samples by plotting a Log2-ratio figure and a Scatter Plot. The procedures were shown as below: (1) Normalize the expression of miRNA in two samples (fetal and adult longissimus thoracis) to get the expression of transcript per million. Normalized expression (NE) = Actual miRNA count/Total count of clean reads. (2) Calculate fold-change and P-value from the normalized expression. Then generate the log2-ratio plot and scatter plot. Fold-change formula: Fold_change = log2 (fetal NE/adult NE). P-value formula:
The x and y represented normalized expression levels, and the N1 and N2 represented total count of clean reads of a given miRNA in small RNA libraries of the fetal and adult stage, respectively. Stem-loop real-time reverse transcription polymerase chain reaction (RT-PCR) with SYBR Green was used for the analysis of miRNA expression
Additional file 11
Stem-loop RT-PCR Primer.
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Competing interests
The authors declare that they have no competing interests.
Authors' contributions
JJS gathered samples, conceived the report, participated in its design, performed data analysis, interpreted results and drafted the manuscript. MJL and ZJL participated in intellectual discussion. JX contributed to gathering samples. XYL, CLZ, CZL and HC provided guidance on experimental design and funding. All authors read and approved the final manuscript.
This study was supported by the National Natural Science Foundation of China (Grant Nos. 31272408, 30972080), Agricultural Science and Technology Innovation Projects of Shaanxi Province (No. 2012NKC01-13), Program of National Beef Cattle Industrial Technology System (CARS-38), and Natural Science Foundation of Jiangsu Province (BK2011206).
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