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Hotel Felix Chicago
Select your stay dates to check availability at Hotel Felix Chicago
Hotel Felix Chicago
About the Hotel
Property Location
A stay at Hotel Felix Chicago places you in the heart of Chicago, walking distance from Holy Name Cathedral and Driehaus Museum. This 4-star hotel is close to John Hancock Center and Millennium Park.
Make yourself at home in one of the 225 air-conditioned rooms featuring iPod docking stations and flat-screen televisions. Your bed comes with down comforters and Egyptian cotton sheets. Complimentary wired and wireless Internet access keeps you connected, and satellite programming provides entertainment. Private bathrooms with showers feature designer toiletries and hair dryers.
Pamper yourself with a visit to the spa, which offers massages, body treatments, and facials. Additional features at this Beaux Arts hotel include complimentary wireless Internet access, concierge services, and a fireplace in the lobby.
Enjoy a satisfying meal at a restaurant serving guests of Hotel Felix Chicago. Quench your thirst with your favorite drink at a bar/lounge.
Business, Other Amenities
Featured amenities include a 24-hour business center, limo/town car service, and express check-in. Planning an event in Chicago? This hotel has 1500 square feet (139 square meters) of space consisting of conference space and meeting rooms. Self parking (subject to charges) is available onsite.
Hotel Facilities
- 24-hour front desk
- Restaurant
- Number of floors - 12
- Meeting rooms 3
- Conference space size (feet) - 1500
- Dry cleaning/laundry service
- Number of buildings/towers - 1
- Spa treatment room(s)
- In-room accessibility
- Laundry facilities
- 24-hour business center
- Express check-out
- Roll-in shower
- Self parking (surcharge)
- Fireplace in lobby
- Television in common areas
- Health club
- Luggage storage
- Limo or Town Car service available
- Free WiFi
- Spa services on site
- Elevator/lift
- Free newspapers in lobby
- Year Built 1925
- Valet parking (surcharge)
- Total number of rooms - 225
- Express check-in
- Full-service spa
- Conference space
- Concierge services
- Smoke-free property
- Breakfast available (surcharge)
- Accessible bathroom
- Tours/ticket assistance
- Multilingual staff
- Coffee/tea in common areas
- Number of meeting rooms - 3
- Use of nearby fitness center (discount)
- Bar/lounge
- Porter/bellhop
- Conference space size (meters) - 139
Room Facilities
- Free dial-up Internet access
- Down comforter
- Daily housekeeping
- Designer toiletries
- Separate sitting area
- Free wired high-speed Internet
- Bathrobes
- Pay movies
- In-room climate control (air conditioning)
- Premium bedding
- Blackout drapes/curtains
- Egyptian-cotton sheets
- Free local calls
- Premium TV channels
- In-room safe
- Air conditioning
- Room service
- Desk
- Flat-panel TV
- Shower only
- First-run movies
- Phone
- Hair dryer
- In-room massage available
- Iron/ironing board
- iPod docking station
- Satellite TV service
- Private bathroom
- Free WiFi
Hotel Policy
Know Before You Go
- Children 12 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.
The following fees and deposits are charged by the property at time of service, check-in, or check-out.
- Breakfast fee: between USD 10 and USD 20 per person (approximately)
- Self parking fee: USD 45 per night
- Valet parking fee: USD 60.00 per night (in/out privileges)
- Early check-in fee: USD 40.00
- Late check-out fee: USD 30.00
The above list may not be comprehensive. Fees and deposits may not include tax and are subject to change.Show all hotel policy Show less hotel policy


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Need a Cholesterol Test in Centerville, UT? Simply order online 24/7 and get a fast, affordable Cholesterol Test at a lab near you (Find More Locations):
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Contact us 24/7: +1 877 678 9627
View product details
Zoom In
- - Width 55" / 140cm - Length 55" / 140cm
Alexander McQueen takes this season's houndstooth and infuses it with signature edge to create this bi-color silk chiffon scarf. Layer it over a leather jacket for a rock 'n' roll finish. Shown here with a Kain tank, Miu Miu pants, Helmut Lang jacket, Jil Sander bag and Rupert Sanderson boots.
- Bi-color silk chiffon scarf with all-over houndstooth print. Alexander McQueen scarf has a skeleton print throughout and a contrast trim. 100% silk. Dry clean.
Product Code: 48465
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Product Code: 48465


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We are supply EDTA 2NA with competitive price , high quality and regular basis.
Specification of EDTA 2NA
English name: Ethylene diamine tetraacetic acid disodium salt(EDTA-2NA)
Molecular Fomula: C10H14N2O8Na2•2H2O
Molecular weight: M=372.24
CAS No.: 6381-92-6
Properties: white crystal powder soluble in water, and a variety of metal ion chelation.
Chloride( Cl)%
Metal chelate(Pb)%
Chelate: mgCaCO3/g
pH Value
Bulk density kg/m3
Clarity testing
white crystal powder
More about EDTA Chemical 99% white powder EDTA-2NA used as additive in textile printing and dyeing
CAS No.: 6381-92-6
Standard: Technical grade
Content: 99%
Loading port: China main seaport
MF: C10H18N2Na2O10
Use of EDTA 2NA
Edetate disodium is an important complexing agent for complexing metal ions and metal separation, this product is used as a color photographic materials bleach-fixing processing, and dyeing auxiliaries, fiber treatment agents, cosmetic additives , medicine, food, agriculture chemical trace fertilizer production, blood anticoagulant, complexing agents, detergents, stabilizers, synthetic rubber, a polymerization initiator and heavy metal quantitative analysis agent. In the reduction with a chlorinating SBR polymerization initiator system, EDTA disodium, as an integral part of an active agent, mainly for complexing iron ions, to control the polymerization reaction rate..
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ED Magedson – Founder
Ripoff Report
Greg Jett AKA Outdoor Lighting Perspectives11718 SE Federal Hwy., Ste. 361 Hobe Sound, Florida United States of America
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Fix it the right way.
Corporate Advocacy Program™
This report was posted on Ripoff Report on 02/01/2012 07:39 PM and is a permanent record located here: http://www.ripoffreport.com/reports/greg-jett-aka-outdoor-lighting-perspectives/hobe-sound-florida-33455/greg-jett-aka-outdoor-lighting-perspectives-gregjett-aka-outdoor-lighting-perspectives-bro-832556. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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SUBMITTED: Monday, April 29, 2013POSTED: Monday, April 29, 2013
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ED Magedson – Founder
Ripoff Report
Mark McClure Ruthless Entrepreneur International6009 W Parker Rd, Suite 149-238 Internet United States of America
Does your business have a bad reputation?
Fix it the right way.
Corporate Advocacy Program™
This report was posted on Ripoff Report on 04/29/2012 02:42 PM and is a permanent record located here: http://www.ripoffreport.com/reports/mark-mcclure-ruthless-entrepreneur-international/internet/mark-mcclure-ruthless-entrepreneur-international-ruthless-entrepreneur-mark-mcclure-marc-875703. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
Ripoff Report has an exclusive license to this report. It may not be copied without the written permission of Ripoff Report. READ: Foreign websites steal our content
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SUBMITTED: Sunday, April 29, 2012POSTED: Sunday, April 29, 2012
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Advertisers above have met our
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*From the University Health Network (Mssrs. Somogyi, Vesely, Preiss, and Correia, and Drs. Azami and Fisher), Toronto General Hospital, and the Department of Medicine (Dr. Fowler), Sunnybrook & Women's College Health Sciences Centre, University of Toronto, Toronto, ON, Canada.
Correspondence to: Robert Fowler, MD, MS, Assistant Professor, University of Toronto, Departments of Medicine (General Internal Medicine) and Critical Care Medicine, Sunnybrook & Women's College Health Sciences Centre, 2075 Bayview Ave, Room D478, Toronto, ON, Canada M4N 3M5; e-mail: firstname.lastname@example.com
Nosocomial transmission of droplet-borne respiratory infections such as severe acute respiratory syndrome (SARS) may be influenced by the choice of oxygen face mask. A subject inhaled saline mist and exhaled through three oxygen masks to illustrate the pattern of dispersal of pulmonary gas. In two commonly used masks, exhaled gas formed a plume emanating from the side vents, while a third mask with a valved manifold, which was modified by adding a respiratory filter, retained the droplets. Maintaining respiratory isolation during the administration of oxygen may reduce the risk of the nosocomial transmission of respiratory infections such as SARS.
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BeschreibungThis booklet, published in cooperation with ISAKOS, is a concise, up-to-date guide and reference on the treatment of cartilage lesions of the ankle that will be of practical clinical value for specialists in sports medicine and sports traumatology. Different techniques and the management of various lesions are described step by step, guiding the practitioner through the entire spectrum of care from evaluation of the injury to successful recovery. Chondral and osteochondral lesions of the talus can present as a consequence of repetitive ankle injuries, causing chronic pain, recurrent swelling, and weakness and stiffness of the ankle joint. An accurate early diagnosis is required, using clinical evaluation, imaging techniques and arthroscopy of the ankle. In acute and non-displaced chondral and osteochondral lesions, conservative treatment is the mainstay. Surgery is recommended for unstable and persistent lesions and consists in several techniques focused on regeneration of the cartilage. Returning to play is possible after testing balance and proprioception, strength, range of motion, and agility.
Inhaltsverzeichnis¿Current concepts and evidence-based treatment of the osteochondral lesions of the ankle.- MRI in the evaluation of osteochondral lesions.- Evolution of surgical treatment of cartilaginous lesions.- Arthroscopic debridement of osteochondral lesions of the talus.- Mosaicplasty of osteochondral lesions of the ankle.- The conservative treatment in Talar OCD: injections of PRP.- Management of the cystic osteochondral lesions.- Osteochondral ankle injuries in footballers.- Treatment of osteochondral defects of the talus in children.- Rehabilitation and return-to-sports activity after debridement and bone marrow stimulation of osteochondral talar defects.
PortraitGian Luigi Canata, MD, is Director of the Center of Sports Traumatology and Arthroscopic Surgery, Koelliker Hospital, Turin, Italy, a position he has held since 1995. In 1999 the Center became an ISAKOS Teaching Center. Since 1980 Dr. Canata has also been a Member of the Medical Commission of the Italian Track and Field Federation and Director of Medical Services and Member of the Board of Directors of CUS (Centro Universitario Sportivo), Turin. From 1985 to 1995 Dr. Canata was Head of the Orthopaedics and Traumatology Section of the Institute of Sports Medicine of Turin. He served as Professor of Kinesiology at ISEF (Istituto Superiore di Educazione Fisica), Turin from 1989 to 2001 and as Professor of Sports Medicine at SUISM (Scuola Universitaria in Scienze Motorie), University of Turin from 2001 to 2012. He is currently President of the Sports Committee of SIGASCOT (Italian Society of Sports Traumatology). He is also a member of the ISAKOS Educational Committee and since 2010 has been a member of the EFOST Executive Board and General Secretary. He has previously served as a member of the ISAKOS Arthroscopy Committee and the ISAKOS Orthopaedic Sports Medicine Committee. Dr. Canata is the author of more than 100 publications.
Niek van Dijk, MD, PhD, is Professor of Orthopaedics and Head of the Orthopaedic Department of the Academic Medical Center Amsterdam, University of Amsterdam. Dr. van Dijk is a specialist in arthroscopic and reconstructive surgery of the knee, foot & ankle and sports medicine. His department has treated many professional athletes from all over the world. He undertook his professional training in Orthopaedic Surgery at the Academic Medical Center of the University Hospital of Amsterdam and in 1994 completed his PhD on "diagnostic strategies in patients with severe ankle sprain." In 1998 he founded the Orthopaedic Research Center Amsterdam (ORCA) and has been Director of the Center since then. He became a full professor in 2002. Dr. van Dijk is a past President of the Dutch Orthopaedic Society, the Dutch Arthroscopic Society and the Nordic Orthopaedic Federation. He is a board member of the International Society of Arthroscopy, Knee Surgery and Orthopaedic Sports Medicine (ISAKOS) and President of the European Society for Sports Traumatology, Knee Surgery and Arthroscopy (ESSKA). Diagnostic and therapeutic strategies for chronic ankle injuries have been a prominent focus of his research. Dr. van Dijk on average presents 25 international invited lectures each year and has published more than 250 scientific articles in indexed publications.
Pressestimmen"There is a growing interest in cartilage preservation and this book is a step-by-step guide to the different techniques and management of various lesions. ... The audience includes sports medicine surgeons and foot and ankle surgeons who treat patients with cartilage lesions. ... With its focus on specific diagnostic tips and surgery techniques for cartilage lesions, this book has an important place in the sports medicine literature." (Pedro A. Iturbide Siles, Doody's Book Reviews, July, 2016)
Untertitel: 50 schwarz-weiße Abbildungen, Bibliographie. Book. Sprache: Englisch.
Verlag: Springer-Verlag GmbH
Erscheinungsdatum: April 2015
Seitenanzahl: 98 Seiten
Format: kartoniert


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Everybody Loves Raiment
What we know about fashion could fit inside one of our C cups, but we do know what we like, and Angela Johnson's Vaudeville Style Mystery Menagerie Fall 2008 Collection Fashion Show at the posh Clarendon Hotel sounds right up our puckered bodice. Johnson says her target market is "young, fashion-forward, daring, indie-minded, artistic women between the ages of 15 and 30 or so" and tells New Times that the 2008 line is "really dark. Almost every piece in the show is black or black and cream stripe."
Like Johnsons fashions, the Mystery Menageries not your typical runway show. DJs Jas and William Fucking Reed will provide the sounds, Pyra Sutra of Scandalesque performs pyrotechnic burlesque dances, and the citys fiercest models will glide and strut down the Clarendons newly remodeled outdoor catwalk. And what will the ladies be wearing? Old vaudeville looks, menagerie/animal inspiration, and murder mysteries, says Johnson. Colonel Mustard, put down that pipe and zip me!
Sat., May 10, 9 p.m., 2008
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City of Pearland, TXHome Menu
Most Popular
Sales and Use Tax
The Texas state sales and use tax rate is 6.25 percent, but local jurisdictions may also impose sales and use tax up to 2 percent for a total maximum combined rate of 8.25 percent.
For additional information about Sales and Use Tax, see
Property Tax
The City's property tax is levied each October 1 on the certified assessed value as of January 1 for all real and personal property. Taxpayers have until January 31 of the following year to pay their tax bill before taxes become delinquent.
The City's current tax rate is $0.6812 per $100 of valuation. The tax rate is adopted in two components as follows:
|Debt Service
| $0.4400
|Operations & Maintenance
| $0.2412
For additional information about Pearland's Property Taxes, see Property Tax Information.


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Perawan Gold High CTR Blogger Template$10.00
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Definition from macmillandictionary.com
Your browser doesn't support HTML5 audio
A computer can store and repeat sequences of instructions.
a dance sequence
Could you describe the exact sequence of events that evening?
The words had to be put into the right sequence.
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Scenes of a film are often shot out of sequence.
action/dream sequences
the famous opening sequence from Reservoir Dogs


text: The plaque shown here commemorates William P. Te Winkle's recognition.To find out more or to contact William P. Te Winkle of Sheboygan, WI, call 920 458-5501, or visit http://www.rohdedales.com. As a result of this honor, American Registry LLC, has added William P. Te Winkle to The Registry™ of Business and Professional Excellence. For more information, search The Registry™ at http://www.americanregistry.com. This press release was written by American Registry, LLC, with approval by Martindale-Hubbell as well as approval and/or contributions from William P. Te Winkle ; it was distributed by PR Newswire, a subsidiary of UBM plc. Contact Information: William P. Te Winkle Phone: 920 458-5501Email Address: email@example.com Website: http://www.rohdedales.com SOURCE American Registry


text:
To sing with us, 1) Click on the music thumbnail icon to view the sheet music (you don't have to read music!), and 2) Engage the audio file by clicking on the Real audio or Mp3 file.
Our hymn this week, "Rejoice, the Lord Is King" encourages us to praise God. Why do we sing praises? We sing praises because God is worthy of praise. We praise Him for all He is and all He has done.
Sing this hymn frequently this week. Acknowledge Jesus Christ as Lord and Savior, and let this hymn fill your heart with praise to the living Lord.
Every Sunday, around 2:15 CST, a Moody radio station near you may feature our weekly hymn lessons. A 5 minute spot is used to highlight our hymn of the week.
Radio lessons for Rejoice the Lord Is King
Weekly: [ Mp3 | Real Audio ]
If you enjoy listening to our hymns online, you can buy a CD and support us at the same time!


text:
|Sale Page
Contact: Wittus Inc.
April and May Sales - 4/1/17 through 5/31/17
|Firebird outdoor grill
The Firebird grill, created by award winning Danish architectural designer Bent Falk, is intended to be used as an outdoor fireplace and barbeque grill, or simply enjoyed as outdoor sculpture.
Made of cor-ten steel which is well known for its durability. It comes un-oxidized but will naturally form a rich reddish brown coating that makes each grill unique. This protects the inner steel and makes it virtually maintenance free for years and years to come.
The Firebird grill has adjustable racks that are made of heavy, solid stainless steel 5mm rods. The innovative design features stay-cool handles, and it even has a built-in warming and frying surface in back where you can simmer sauces or sauté vegetables.
The chimney create a strong draft that allows you to build faster fires. Then it "funnels" the smoke up and away from the chef – and nearby guests.
The Firebird comes with removable stakes to secure them on a lawn or at a beachfront location. And when winter rolls around, light up a roaring wood fire as a warm gathering place après skiing, skating, or sledding.
The Firebird measures 4'3" h x 19"w x 19"d and weighs 160 lbs.
Firebird Grill: sale price-$1,030;
Larger image
Firebird Grill
QuickFire Fire Starters
The QuickFire Fire Starter packets facilitate both safe and efficient lighting – for wood burners and barbecue grills.
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Trendline 3
Standing an impressive 44 inches tall, the Trendline 3 is elegant and understated. Available in black steel with soapstone cladding.
It is a fine example of Danish craftsmanship and eye for detail: door hinges are invisible as is the chrome-finished ash drawer tucked within; the lower cabinet door conceals the storage space without tell-tale hinges and with a quiet, magnetic "latch". It features the exclusive DuplicAir — a revolutionary air intake control system.
For further specifications refer to the
Wood Stove Catalog.
sale price: Trendline3 in black steel with soapstone - $4,350
Pilar – Wood burning stove
The Pilar is a time-tested woodstove with an eye on today's home fashion tastes. Tall, slim, and a round shape with a rotating base, which can be turned through 70˚, 160˚ and 360˚.
Vesta Award winner, the Pilar is surprisingly compact with an 18" (46cm) diameter and just over 67inches (172cm) tall – and it provides welcoming warmth in the home. Heats up to 23kBTU (7kW) or an area of 1,100 sq. ft (100sq. m). A drawer for the wood storage area is also a nice touch.
The rounded glass doors provide a unique view of the fire. Perfect as a room divider. 76% efficient.
sale price: $5,830
With an upward flourish, the impressive Etna commands attention with its curvaceous, organic architecture flanked by wings of soaring sculptural tiles. These tiles are manufactured using a highly advanced manufacturing process and are designed to retain and then slowly release heat into the room.
It measures 58" high x 39" wide x 23" deep and weighs about 850 pounds. Etna has a huge 19" x 15½" fire window that glides effortlessly up and down via a silent counterweight system. A push-button finger latch releases the door outward for ease of cleaning.
A slide out storage drawer below to house wood, tools, kindling. High-grade steel housing in gray. The Etna heats up to 38,000 BTUs; 1,900 square feet. Top or back vented. Efficiency tested at 78%.
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Jennifer Love Hewitt Rounds the Bases on "The Client List"
Getting sporty on the set of her Lifetime series, Jennifer Love Hewitt filmed for "The Client List" at a baseball field in Los Angeles on Monday (March 18).
The "Heartbreakers" star looked cute in her blue top, navy zip-up sweatshirt, matching cap, and blue jeans, while she shot with costar Brian Hallisay as their characters looked about to round a few bases of their own.
Though she looked energized, the 34-year-old actress's day started extra early. On Monday, she tweeted, "Good morning! 3:45 am wake up so brutal. Busy day at work today. So glad you guys watched last night."
As previously reported by GossipCenter, Ms. Hewitt recently had to put to bed some crazy rumors about herself, tweeting on Thursday, "I'm insuring my boobs! I'm freezing my eggs! Nope I'm shooting the new season of 'The Client List!' Now that isn't made up ;)."
Enjoy the pictures of Jennifer Love Hewitt filing scenes for "The Client List" in Los Angeles, CA (March 18).
Photo Credit: Wenn.com


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Audio: Rick Perry's "Mr. Way-Too-Liberal-for-Texas Guy" ad; Update: Similar ad in Minnesota
posted at 7:53 pm on October 27, 2006 by Allahpundit
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Link shamelessly stolen from Mary K, who shamelessly stole the Matthews video from us this morning. I don't know what Chris Bell looks like and I don't care enough to check, but since we need a mental image of Mr. Way Too Liberal for Texas Guy, this'll do. Click to listen.
Update: Tim Pawlenty's running basically the same ad in Minnesota. It's not as good as Perry's, though.
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I voted today, but not for Chris Bell. As I hit the button for Governor several times to the tune of "Way Too Liberal for Texas Guy!", the reply was "SSSHHHHHHH!!!".
Actually he doesn't look that dissimilar from Chris Matthews. Same distraught, wussy look on his face and everything.
Lone Star on October 27, 2006 at 8:01 PM
Clark1 on October 27, 2006 at 8:15 PM
Je m'appelle Chris Bell!
So much goodness in that clip.
Citizen Duck on October 27, 2006 at 8:19 PM
This just typifies the kind of baseless attack coming out of the AllahPundit camp these days. Frankly, it's disappointing.
I think the people of Hot Air and Townhall would be better served if we could get back to the real issues of this campaign season, like posting inane campaign commercials and offering election predictions we will change three days from now. You know? The substance.
I sincerely hope AllahPundit will join me in this effort. After all, this election is not about our personal disagreements; it's about entertaining, even bludgeoning, our readers with other people's personal disagreements.
I'm Mary Katharine Ham and I approved this message.
(And, I totally didn't steal it.)
marykatharine on October 27, 2006 at 8:20 PM
This just typifies the kind of baseless attack coming out of the AllahPundit camp these days. Frankly, it's disappointing.
Not to mention racist.
The background color on this page, as you surely have noticed, is white, while the font color is quite clearly, black.
Case closed.
Citizen Duck on October 27, 2006 at 8:23 PM
Saw screenshot, Googled beady-eyed.
First entry gives this:
Adj. beady-eyed – having eyes that gleam with malice
malevolent, malicious – wishing or appearing to wish evil to others; arising from intense ill will or hatred; "a gossipy malevolent old woman"; "failure made him malevolent toward those who were successful"
Stephen M on October 27, 2006 at 8:27 PM
I'm a native Texan and a pretty straight ticket Republican, but I'm not voting for Perry–he "lowered" property taxes by raising the tax on cigarettes and on businesses and corporations (Greeeaaatt….), he wants to build the Trans-Texas Corridor, a massive boondoggle like the Big Dig that would involve big time eminent domain seizures, major graft, would run into Mexico (we all know what that will lead to! Who needs a fence when they can drive into the U.S.?) and be run by foreigners.
Folks are pretty unhappy about Rick welcoming so many of New Orleans criminals to Houston, where the murder and crime rates have skyrocketed.
All my friends have one word to describe Perry: Arrogant.
The race is between Kinky Friedman and Grandma Carole Strayhorne. Noone's paying Chris Bell any attention.
Jen the Neocon on October 27, 2006 at 8:27 PM
It's a good, humorous ad but I wonder why Perry is running this. Seems like a gratuitous, mildly-negative ad that's being run just for the heck of it. Perry's had a 2-1 lead in the polls over all of the other candidates, and I think Bell has always polled fourth of the four. Bell received some very large donations recently from some of the wealth personal-injury lawyers (John O'Quinn) recently, but that's still not going to get him close. Anyway, just makes me wonder why Perry is directing negative ads towards Bell of all of his opponents. (I held my nose and voted for Perry today.)
dhawbake on October 27, 2006 at 8:30 PM
That was pretty damn funny.
As a Texan and someone who works in law enforcement it will be a cold day in hell before I vote for Bell, but Perry is not fit for the job eaither.
Its time to get Kinky!
SnakeintheGrass on October 27, 2006 at 8:33 PM
This just typifies the kind of baseless attack coming out of the AllahPundit camp these days. Frankly, it's disappointing.
You're only disappointed because I'm white.
Allahpundit on October 27, 2006 at 8:38 PM
Kinky? Kinky?….you're in law-enforcement in Texas?…..
Kinky?……………guess you won't be searching Willie's bus next time it crosses the border…….
Kinky?…(I'm a Texan too………but Kinky?)
Limerick on October 27, 2006 at 8:42 PM
It's a good, humorous ad but I wonder why Perry is running this. Seems like a gratuitous, mildly-negative ad that's being run just for the heck of it.
Oh for Pete's sake, what is wrong with that ad? Seriously?
We all seem to fixate on saying "I don't approve of negative ads", but really, what does that mean?
Are we never allowed to mention an opponents position?
If a candidate wants to raise taxes, are we allowed to point that out? If we do, isn't that negative? You are pointing something out about an opponent that isn't favorable to him.
If an opponent wants to cut and run, aren't we allowed to point it out?
And lets face it, politicians run negative ads because they work. Just as we want to know what is right about our candidate, we want to know what is wrong about our opponent. We need that negativity, in order to feel good about our choice.
Now I'll say there should be a limit. First, a negative ad should be true. Don't lie. Second, don't go after his family, unless they are actually actively involved politically. Third, when you hammer an opponent on an issue, you had better be providing the public with YOUR position on the issue, and what you would do differently.
And try to be amusing, or interesting. Cuz if your ad is boring, you ain't getting elected.
EFG on October 27, 2006 at 8:46 PM
You're only disappointed because I'm white.
Allahpundit on October 27, 2006 at 8:38 PM
Actually, you are Italian. Which is kinda ethnicy.
Which automatically puts you under suspicion.
EFG on October 27, 2006 at 8:49 PM
Kinky has about five minutes of material that he repeats over and over and over…….
Scot on October 27, 2006 at 8:50 PM
He is running it because Bell is running an add that says directly the Perry is 'corrupt' because the children's health insurance here is being managed by a off shore company……..just typical Texas hard ball. Perry 35%, Strahorn 20%(I), Bell 20%, Kinky (10%…Perry is a shoe-in but with less then 50%
Limerick on October 27, 2006 at 8:50 PM
Great, now I'm gonna have that damn song stuck in my head the rest of the night.
*(raises paw)* …another Texan….
I'm surprised…first of all that Bell's actually running a commercial (I haven't heard n'r seen anything from him, the only evidence of his existence being rumor)…secondly that Perry ran an ad which is actually clever. He's been governor here for four years and strikes me as an empty suit. As Jen the Neocon wrote, he's behind some things which're raising some eyebrows here'bouts…in my little corner of the world, anyway.
Maybe we'll get Kinky here in Texas…I doubt that Gradma Carole will get the nod, which is unfortunate…should be interesting….
Puritan1648 on October 27, 2006 at 9:05 PM
True that Perry has his problems….I don't have a poster up of him in the garage. Bell has been putting his ad's on in DFW, Austin, Houston markets and this week made the local news shows shoveling his pitch(shoveling being the operative word). I don't like any of the candidates here, but Perry will win.
Limerick on October 27, 2006 at 9:09 PM
Kinky? Kinky?….you're in law-enforcement in Texas?…..
Kinky?……………guess you won't be searching Willie's bus next time it crosses the border…….
Kinky?…(I'm a Texan too………but Kinky?)
I enforce the laws. Dosent mean I have to agree with them. Weed is no more dangerous than cigarettes, if that is what you are getting at. I know guys in DPS who feel the same way.
Am I saying that Kinky is the best man for the job? No. He just happens to be the lesser of of four evils.
SnakeintheGrass on October 27, 2006 at 9:18 PM
Snake that was NOT my truck that just headed west at 95 ok?
Limerick on October 27, 2006 at 9:21 PM
Hey fellow Texans!
I meant to say that it was a 3-way race between Perry, Strayhorne and Kinky, but Perry probably will win. Puritan, he's an empty suit indeed!
And Scot made a great point: Kinky's hilarious, but he does same the funny things over and over.
As much as I love Bush, being governor of Texas ain't no real thang, although Lord knows Perry's tried to make it a big deal and I'm sure he thinks there's a presidential run in his future, but he's no George Bush.
Hell, he's more like Ann Richards and that's not good.
Jen the Neocon on October 27, 2006 at 9:22 PM
Snake that was NOT my truck that just headed west at 95 ok?
So you don't mind if I pull said truck over, then? :P
I meant to say that it was a 3-way race between Perry, Strayhorne and Kinky, but Perry probably will win.
Unfortunatly you are probably right…
SnakeintheGrass on October 27, 2006 at 9:26 PM
Yeah it is hard to trust an ex-Dem running as a Republican…
Limerick on October 27, 2006 at 9:28 PM
You're only disappointed because I'm white.
Allahpundit on October 27, 2006 at 8:38 PM
Actually, you are Italian. Which is kinda ethnicy.
Which automatically puts you under suspicion.
EFG on October 27, 2006 at 8:49 PM
OK OK OK- AP's White (could be a lie, it is election time) he's 1/2 Italian and half of something else…(hence the earlier half-breed jab) what could that be?
WE'RE GETTING CLOSER- MKH will tell us, with all this business nepotismistic tension. (dont know if that's a real word, but it works, kinda)
The other half of his ethnicity is Irish.
And you know how we WASPs feel about the Irish
Plus he is Catholic. Which means he is a blasted papist.
Hardly. Under the three strikes and you're out rule, (Italian/Irish/Catholic) AP has been officially deemed a minority.
But were're a big tent…
EFG on October 27, 2006 at 9:56 PM
If Kinky is serious about sealing the border, he would block the NAFTA superhighways. I would vote for Kinky if I still lived in TX.
I' from Texas and I'm gettin KINKY!!!!!!
TexasInfidel on October 27, 2006 at 10:00 PM
Yeah but he wants to seal the border by pulling all the troops out of Iraq and bringing them to Texas….
Texas is weird; Perry- exDem running as Republican
Grandma exRep running as Independent
Kinky- exDem running as Independent
Bell- Dem running as Nutroot
No wonder y'all have no confidence in us!
Limerick on October 27, 2006 at 10:02 PM
Allahpundit Say's
( I don't know what Chris Bell looks like )
He looks just like the picture you get in your head when listening to that Rick Perry ad.
Texyank on October 27, 2006 at 10:35 PM
Well, I'm late to the party, but *raising MY hand as a Texan* I'm probably going to hold my nose and vote for Perry. He's the most normal one of the bunch, even though I don't think he's done shit in the last few years.
Besides, the Governor of Texas doesn't hold the power – the Lt. Gov. does.
but I have to say, Kinky is an interesting alternative…
pullingmyhairout on October 27, 2006 at 10:43 PM
(I held my nose and voted for Perry today.)
How could you have voted for Perry? I would rather have a idiot like Kinky in office than the greasy Rick Perry. Perry is going to cut up Texas into tiny little pieces.
riggermarander on October 27, 2006 at 10:50 PM
Sorry. I missed the link.
riggermarander on October 27, 2006 at 10:52 PM
Pile it on. I am new to this posting thing. I am usually just lurking around.
riggermarander on October 27, 2006 at 10:55 PM
Voted for Grandma Strayorn…she shares my pathological hatred for toll roads, trans-Texas corridors and as she says, she's too damn old to worry about feathering her nest and making a career out of being Governor. Perry will probably get it with only 40% or so, but I damn well hope Strayhorn makes him sweat for it.
Texan on October 27, 2006 at 11:56 PM
I got a mailer today from the perry camp going after strayhorn for being pro-choice. That will probably bring a lot of republicans back home to perry. I've seen a lot of kinky signs and bumper stickers in my neck of the woods though.
Scot on October 28, 2006 at 12:24 AM
The corridor issue is definitely what's hurting perry the most.
Scot on October 28, 2006 at 12:30 AM
Ain't early voting great? I would have voted already if it weren't for my uncertainty about the governor's race.
I would have voted for Werner, but the post-9/11 world of the more-unabashedly-unhinged-every-day Democratic Party is not the place to be diverting votes from Republicans, so my days of voting straight-ticket Libertarian, I'm afraid, are over. I'd be inclined to go for Perry if it weren't for his damn Trans-Texas Corridor land-grab monstrosity. I'd consider Friedman if I thought he might be anything more than an entertainer (although there's certainly something to be said for having an entertainer in the governor's mansion). All things considered, I rather favor Strayhorn except for my fear of helping siphon off enough votes from Perry to let Bell win. For the love of God, Montresor, anyone but Bell!
Looks like I've got some more research to do before Monday.
BTW: Just how the hell many Texans are there around here, anyway?
p.v. cornelius on October 28, 2006 at 1:38 AM
I too am a Texan (DFW area). I see Perry as mostly an empty suit, but nostly a decent, right-leaning moderate soldier. The Texas governor just does not matter that much. I hate toll roads too; but we have them, and they're not going away. The trans Texas corridor is ultimately just a highway. The decision to enforce the border (incl. the TTC) will be implemented and enforced when the next major attack happens on US soil by someone who came across the US-Mex border.
I didn't know Strayhorn was pro-choice, but I wasn't going to vote for her anyway. Kinky is literally a clown. Bell who? Unfortunately, we don't currently have any conservative visionaries right now, for which we are duly ashamed, and which is why you hear so much about holding noses while voting.
urbancenturion on October 28, 2006 at 3:11 AM
I'm not sure what I enjoyed more; the brilliant and funny ad or MKH's politically satirical comment to AllahPundit's post (pbuh).
iNeXuS on October 28, 2006 at 6:00 AM
I can't add anything to this.
daveintexas on October 28, 2006 at 8:19 AM
except I could have made it a link, duh
daveintexas on October 28, 2006 at 8:19 AM
riggermarander: "dhawbake, how could you have voted for Perry?"
Very simply because I want a governor who will leave the death-row execution express lane open (characterization shamelessly stolen from Blue Collar comedian Ron White). I think Perry will at least do that, and I have no confidence that Kinky wouldn't shut it down, or even commute all death-row sentences to life in prison like that crook-idiot, Ryan, did in Illinois. And "Grandma" Strayhorn-Keeton-Rylander (or whatever her last name is this week) is nothing more than a self-promoting idiot. Like I said, I held my nose. There just aren't any good choices this year.
EFG: I have no problems with negative ads per se as long as the content is a fair portrayal of the facts. I think Allen's ads are going to be effective and reduce support for Webb, regardless of what MM says. My question about this ad is not that it's negative – it's why is Perry spending money attacking the fourth place candidate? Seems odd. (Yes, I know, Perry is odd.)
dhawbake on October 28, 2006 at 9:06 AM
I like Kinky as a personality, but not as a governor. Pro-gay, pro-indian casinos (wouldn't you like to get your hands around the throat of the judge who made THAT possible), pro-DRUGS?? In Texas??, lip service about National Guard on the border…yada, yada, yada.
I can't THINK of a quicker way to get Texas looking like California.
Most disturbing Texas election revelation to me: Willie Nelson is a drug user. I always heard he told his band, "If you're wired, you're fired." Then he gets pulled over with a pound of pot in his RV.
You pretty much HAVE to vote for Perry. Voting for anyone else is a Donk proxy.
Left_Behind on October 28, 2006 at 9:38 AM
Bell looks like Dr. Drew
Drtuddle on October 28, 2006 at 11:52 AM
I think it's a very clever ad based on the same format as a very successful product ad and it's funny and accurate! And Matthews does strikingly resemble Chris Bell!
Poor Bell, he got kicked out of the legislature because of DeLay's re-districting plan. He got the nod in the primary based on a sympathy vote. The Dems picked a sorry, whiner for a candidate and I'm glad they did.
The Dems fight dirty in Texas. They're trying so hard now to get Lampson elected in DeLay's district they've made it just as complicated as possible to elect DeLay's designated replacement Sekula-Gibbs. And Lampson might just win it, but District 22 will come back Republican in the next term.
I'm voting for Perry next week in early voting. I'm not going to contribute to Texas being taken back by the Democrats and I want Chris Bell to get his butt kicked big time. By holding on to the Governor this term we have a real comer in David Dewhurst for the 2010 election. He'll make a great Texas Governor.
Grandma pissed me off when she ran her ad saying most seniors in Texas got no property tax reduction when in fact after a certain age they PAY NO property tax in Texas. And as the former Comptroller, she surely knows that. I think it's despicable to run as a "grandma" and then use that persona to portray herself as trustworthy when that ad is deceitful. Speaks about her character. I'm sick of that "let's scare the old people who are to ignorant to know better" tactic.
Kinky, however amusing he is, would be a disaster for the State.
Oh BTW, it was Houston Mayor Bill White (D) is responsible for creating the crime problems that have resulted from the NO evacuees in Houston. He wanted the federal $$$$$ to offset his budget problems. The longer he kept them in the Dome and Geo. R. Brown, the more money he got. It should have been a temporary pass through as they were moved on to other places like they did in Dallas and San Antonio.
Texas Gal on October 28, 2006 at 11:54 AM
I voted for Perry yesterday, with no qualms. Kinky is just comedy fodder for this election. I wouldn't mind a road through Texas that bypasses Austin and San Antonio, since the traffic is horrendous on I-35.
Stormy70 on October 28, 2006 at 12:31 PM
Texas Gal,
Is Sekula-Gibbs related to Jay Sekula of the ACLJ? (attorney who argues conservative side in US Supreme Court cases for ACLJ)
I looked on her website and didn't see any personal info.
I almost forgot about the scam against Delay….Man, what a year!
Left_Behind on October 28, 2006 at 12:52 PM
As a native Houstonian, let me correct you, please.
The Governor of Texas cannot commute death sentences and it has been that way since Ma Ferguson was governor. The Governor can stay an execution for 30 days. Texas governor has little or no real power – that falls to the Lt. governor.
Neocon Peg on October 28, 2006 at 1:24 PM
I don't really know if Shelly is related to Jay or not. If she is and if Lampson could put negative spin on it, he surely would have made it public knowledge.
He's got this ad running that shines a spotlight on a Houston City Council Chair and says that Sekula-Gibbs sat in that chair and took a $5000 raise while Houston faces a $87,000,000 budget shortfall! And I'm screaming at the TV, Wadda ya mean 87 freaking million dollars??? Shelly's been there one term and the damn Democrat y'all elected Mayor is in his 2nd freaking term!!!!
Texas Gal on October 28, 2006 at 1:33 PM
The Trans-Texas Corridor is NOT just a "road" to help with traffic. This is a gigantic land grab that will take millions of prime Texas farmlands away from Texans who have owned it for generations. Then Perry will turn all of those millions of acres over to a Spanish company to collect the tolls. The toll "road" is only part of this. This will go down as the biggest land grab in United States history. Itis pathetic and shameful.
Perry does not deserve my vote and he won't get it!
Neocon Peg on October 28, 2006 at 1:41 PM
Oops, Sorry, forgot the link
Neocon Peg on October 28, 2006 at 1:44 PM
Bell is a non-factor.
Kinky is funny, but not a serious alternative.
Grandma is plausible, but not viable.
Perry has a record, but it is an uneven one.
Still, it was a funny ad, and from across the Red River here in Stephens County, I hope that my pals in
South Oklahoma Texas keep Perry around a bit longer.
lol….Thanks Texas Gal.
Left_Behind on October 29, 2006 at 1:58 AM
On the Texas elections:
Sekula-Gibbs and her opponent are tied as of this morning. Her name is hanging on the wall for anyone to see so they can spell it right; and you don't even have to spell it right for the vote to count. A one-eyed retard with only 2 fingers could figure out how to vote for her. I won't be at all surprised if she wins; she has my vote.
As for our current Governor, Mr. Rick "Let's Leave the Border Wide Open Until Election Day" Perry; he still gets my vote unless Streyhorn pulls ahead of that whack-job Democrat… which she almost has. Strong borders are nothing more than an election day game for Rick Perry. He should count himself very lucky that he has a Democrat and an Independent running at 22% and 21% respectively.
Cary on October 30, 2006 at 9:22 AM
Perry is a failure in the most important issue in
Texas Tejas…immigration. I haven't decided between the Grandma and the Comedian but doubt it will matter. Perry will win but I hope to at least send a "I'm unhappy with the Republican leadership" message.
Here's my own middle names for all the gov candidates of Texas:
Rick "The Hair" Perry (R)
Chris "Taco" Bell (D)
Kinky "Sex" Friedman (I)
James "Smoke em" Werner (L)
Carole "Competing with Liz Taylor" Keeton McClellen Ryander Strayhorn Smith Rodgers Williams Osborne Jackson Newton Whitaker Jones Montgomery Gibson Chambers Parker….. Etc. (I)
ricer1 on October 30, 2006 at 4:53 PM
Oh, and one write-in candidate:
James "Patriot" Dillon
p.s., That's his own "middle" name… or so he claims.
ricer1 on October 30, 2006 at 4:55 PM


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|Re: ActiveMQ 4.1.1 server freezes
|Thu, 08 May 2008 19:41:06 GMT
Daryl Richter-3 wrote: > > Do you open and close multiple sessions within the same consumer thread? > > We have seen that this can very reliably cause the broker to lock up. > > It is best to open a single session per thread and close all the way out > when done consuming. > Is it wrong to open multiple sessions from the same thread? What we typically do is in one of our application threads, create sessions and register a single consumer with a registered message listener on each. Likewise we'd create a separate session for each producer we'd need. -- View this message in context: http://www.nabble.com/ActiveMQ-4.1.1-server-freezes-tp17104702s2354p17134918.html Sent from the ActiveMQ - User mailing list archive at Nabble.com.
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This is just a demo map that you can delete right away, if you feel like it...
METS.9-12.CI.1 Activity: Apply advanced software featurees to redesign the appearance of word processing documents, spreadsheets, and presentations
Example: Have students use a thesaurus, templates, and different styles to update a resume.
METS.9-12.RI.8 Activity: Research examples of inappropriate use of technologies and participate in related classroom activities.
Example: Have the students perform online research, perform interviews, identify sources, and evaluate information for a mock trial.
METS.9-12.CT.3 Activity: devise a research question or hypothesis using information and communication technology resources, analyze the findings to make a decision based on the findings, and report the results.
Example: Have the students participate in a research project and then present it to the class using technology.
METS.9-12.CC.1 Activity: Use available technologies to communicate with others on a class assignment or project.
Example: Have the students conference with eachother using SKYPE with both video chat and instant messaging.
METS.9-12.DC.3 Activity: Discuss and demonstraate proper netiquette in online communication.
Example: Have the students read an online article, then respond to a class blog and use citations when quoting during their posts.
METS.9-12-TC.1 Activity: Complete at least one online credit, or non-credit, course or online learning experience.
Have the students take an online Blood Borne Pathogens tutorial and then complete the online quiz.


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Wireless BCM4312 with the 3.10, 3.11, 3.12 and 3.13 kernels
The hybrid driver from Broadcom was updated again in September (2013) with all the previous patches and a few other fixes as well. This brings them up to support linux kernel 3.9, which is very much welcome.
Sadly it breaks again with >= 3.10 with many warnings and errors which isn't good considering that Ubuntu (13.10) Saucy Salamander is about to be released.
We do have a patch for you though that gets us working again up to the 3.11 kernel.
Chipsets supported by "Broadcom's IEEE 802.11a/b/g/n hybrid Linux® device driver" are: BCM4311, BCM4312, BCM4313, BCM4321, BCM4322, BCM43224, BCM43225, BCM43227 and BCM43228.
Worldsynth 0.11.0 released
Worldsynth version 0.11.0 is released and can be found on github.
In this release we've added an additional algorithm for heightmap generation based on Ken Perlin's work in noise generation. We decoupled the sea-level to be configurable based on percentage, which in addition to masks we can now create islands. You can also save your world and open it up later since we use pytables to store our settings, metadata and our data in an open hdf5 format. You can also export your heightmap in 16-bit PNG greyscale or even import from a wide array of images formats as a heightmap. Importing from an image creates a 16-bit precision greyscale heightmap. In addition to this, one commenter ask about Python3 support, well now you have it.
Worldsynth 0.10.0 released
Worldsynth version 0.10.0 is released and can be found on github. This is our first "official" release in which the result should work, out of the box, with a usable and familiar GUI instead of the pygame environment. This is provided by Qt4 via PySide. We have even tested Worldsynth on Windows XP to validate that it is indeed cross platform.
As for 0.11.0, we are looking to unlock size of terrain to be of any variable width and height instead of the basic power of two. We are also investigating fluvial erosion.
Latency simulation over long fat network
One of our clients asked us how we handle latency, and not just a few ms across racks but 2 and even 3 digit ms latency that indicates geographically separate locations across continents, not just a country. Not only that, the "pipes" involved are 10Gbps and we had to fill them. We have the theories and made models of how it would work. We perhaps might not be able to fill a 10Gbps fully with one stream, we could fill it with multiple streams but we had to validate this conclusion.
The question now becomes, how do we test this. We've done our research and there are only a few commercial solutions available like the Netropy 10G2 which is a 4 port, 2 lane hardware latency simulator for $30,000 new. Not only is that outside my budget, it is still limited to simulating 2 10Gbps pipes while we need at least 3 lanes (6 ports) and possibility to expand to more as necessary. We decided it was cheaper in terms of total cost to put the research into creating our own Latency Simulator.


text: Cowboys eke out OT victory over Redskins By Gary Graves, USA TODAY LANDOVER, MD. – Just because the Dallas Cowboys and Washington Redskins are on opposite trajectories didn't mean their rivalry couldn't have drama. There certainly was plenty in Sunday's back-and-forth game that Cowboys kicker Dan Bailey ultimately clinched with a 39-yard field goal at 9:21 of overtime for a 27-24 victory. His kick, which gave Dallas its third consecutive victory, came moments after counterpart Graham Gano missed wide right on a 52-yard attempt that could have capped the Redskins' second rally after Rex Grossman's four-yard touchdown pass to Donte Stallworth with 22 seconds remaining forced overtime. In between was Tony Romo's clutch play that set it up, a 26-yard pass to Dez Bryant on third-and-15, followed by the Cowboys quarterback's mental lapse that could have jeopardized the field goal attempt when he stood and motioned for a timeout that his team didn't have. That would have resulted in a penalty. http://www.usatoday.com/sports/story/2011-11-20/cowboys-redskins/51322126/1


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Stocks May Open Lower Amid Likely Profit Taking - U.S. Commentary
RTTNews - Following a notable run-up last week, stocks are looking to open lower on Monday as traders may look to do some profit taking. The major index futures are all in negative territory, with the Dow futures down by 36 points.
The likely pullback to kick off the week comes amid the absence of any major catalysts, as traders may pause and move to the sidelines to contemplate their next moves. Later in the week, however, market focus will turn to some key economic reports, including data on retail sales, industrial production and weekly jobless claims.
On the corporate front, Freddie Mac (FRE) said late Friday that it no longer needs government help at this point of time, as the mortgage lender posted a positive net worth. Benefiting from accounting adjustments and gains, the company reported a second quarter profit of $821 million for the quarter on a notable increase in revenues to $7.47 billion from last year's $1.59 billion.
Warren Buffett-owned Berkshire Hathaway (BRKA) reported a 14 percent increase in second quarter earnings, fueled by gains from derivatives trading, which soared to $1.53 billion from $453 million in the second quarter of last year. The firm earned $1,147 per share, while Wall Street analysts expected the company to earn $1,238 per share for the quarter.
Microsoft (MSFT), which recently struck a ten-year deal with Yahoo (YHOO) to share search engine technology and ad revenue, announced an agreement with French ad company Publicis to dispose off its Razorfish digital advertising unit, which it had acquired as a part of its acquisition of aQuantive.
Meanwhile, McDonald's (MCD) announced that its global comparable sales rose 4.3 percent in July, benefiting from a 7.2 percent jump in sales in Europe. U.S. sales rose 2.6 percent primarily due to new products, including McCafe espresso-based coffees, and the continued strength of McDonald's core menu.
Stocks saw a notable rally on Friday, fueled by the day's encouraging employment data. The Dow closed up by 114 points at 9,370, the Nasdaq climbed by 27 points to 2,000, and the S&P 500 rose by 13 points to 1,010.
With the gains, the major averages all closed higher for the week, marking the fourth consecutive week of gains. The Dow and the S&P 500 posted weekly gains of 2.2 percent and 2.3 percent, respectively, while the Nasdaq rose by a more modest 1.1 percent for the week.
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Timbaland ft. Veronica Gardner - Give It a Go
By richard | 5 years ago
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Artist: Timbaland
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text:
Jenny's Alter-Ego
Should I tell him? I don't know. Is it really what I want to do - to alienate one of the few people actually talking to me? I mean, he seems like a nice guy, but I'm sure he shouldn't be thinking that.
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text:
Exploring the molecular causes of hepatitis B virus vaccination response: an approach with epigenomic and transcriptomic data
Genomic epidemiology
Variable responses to the Hepatitis B Virus (HBV) vaccine have recently been reported as strongly dependent on genetic causes. Yet, the details on such mechanisms of action are still unknown. In parallel, altered DNA methylation states have been uncovered as important contributors to a variety of health conditions. However, methodologies for the analysis of such high-throughput data (epigenomic), especially from the computational point of view, still lack of a gold standard, mostly due to the intrinsic statistical distribution of methylomic data i.e. binomial rather than (pseudo-) normal, which characterizes the better known transcriptomic data.
We present in this article our contribution to the challenge of epigenomic data analysis with application to the variable response to the Hepatitis B virus (HBV) vaccine and its most lethal degeneration: hepatocellular carcinoma (HCC).
Twenty-five infants were recruited and classified as good and non-/low- responders according to serological test results. Whole genome DNA methylation states were profiled by Illumina HumanMethylation 450 K beadchips. Data were processed through quality and dispersion filtering and with differential methylation analysis based on a combination of average methylation differences and non-parametric statistical tests. Results were finally associated to already published transcriptomics and post-transcriptomics to gain further insight.
We highlight 2 relevant variations in poor-responders to HBV vaccination: the hypomethylation of RNF39 (Ring Finger Protein 39) and the complex biochemical alteration on SULF2 via hypermethylation, down-regulation and post-transcriptional control.
Our approach appears to cope with the new challenges implied by methylomic data distribution to warrant a robust ranking of candidates. In particular, being RNF39 within the Major Histocompatibility Complex (MHC) class I region, its altered methylation state fits with an altered immune reaction compatible with poor responsiveness to vaccination. Additionally, despite SULF2 having been indicated as a potential target for HCC therapy, we can recommend that non-responders to HBV vaccine who develop HCC are quickly directed to other therapies, as SULF2 appears to be already under multiple molecular controls in such patients. Future research in this direction is warranted.
DNA methylation (addition of a methyl group to the 5th carbon cytosine residues in CpGs islands) is stably maintained, inheritable and regarded as an epigenetic marker, which augments stability and diversity of biological phenotypes, yet without modifying the genomic sequence. DNA methylation not only plays a crucial role in a spectrum of physiological processes, such as gene imprinting and X-chromosome inactivation
Bisulfite-conversion based approaches are widely used for DNA methylation measurements, and exploit both microarray and sequencing technologies, as it is the case for other
The degree of methylation is usually denoted as β, ranging from 0 to 1. Methylation data are presented in the same matricial form of expression data (locus × sample), however, cautions must be used in the direct application of transcriptiomic analysis tool to methylation data. In particular, the assumption that most genes are not differentially expressed no longer holds for methylation data: in the human genome 70% to 80% of CpGs are methylated to various extents
Despite these difficulties, the ubiquity of methylation phenomena makes them interesting candidates to explain number of open clinical problems. In particular, Hepatitis B virus (HBV) vaccine is an effective prevention of HBV infection, yet not all people can benefit from it because of varying degrees of responsiveness. We have already shown
Given the importance of the clinical phenomenon (infection rate in Southeast Asia, parts of China and tropical Africa above >8%
Ethical statement
The study protocols and consent procedure were approved by The Medical Ethical Committee of Children's Hospital of Fudan University, Shanghai, China. Written informed consent forms were obtained from parents on the behalf of the participants involved in the study, conducted in accordance with the guidelines proposed in the World Medical Association Declaration of Helsinki.
Whole-genome DNA methylation data & study subjects
All subjects were recruited at their 1-year-old regular physical examination performed at the children-care clinics of five comprehensive hospitals in Urumqi (Xinjiang Uygur Autonomous Region, China) after they received the 5 μg recombinant HBV vaccine recommended by the Chinese Ministry of Health (KT60, 2004 L00065, Kangtai Biological Product, Shenzhen, Guangdong, China). Sampling was done at each of the three injections, following the national 0-1-6 HBV vaccination schedule. The inclusion and exclusion of subjects, HBV biomarker examination and data collection are described elsewhere
Twenty-five infants were selected for genome-wide DNA methylation analysis. Thirteen of these were non- or low-responders to the vaccine (cases, anti-HBs < 100mIU/ml) and 12 were normal responders (controls, anti_HBs > 500mIU/ml). Whole blood (2 ml) was collected for testing DNA methylation levels using the Illumina HumanMethylation 450 K microarray, processed and filtered according to the standard Illumina protocol in 2 batches: the first with 17 samples and the second with 8 samples. Clinical data are listed in Additional file
Additional file 1
Sample labels. Summary of clinical parameters.
Click here for file
Methylomic data preprocessing
Quality Control (QC) assessment was performed with the open-source R package
Figure 1
MDS plot
MDS plot. Multiple dimensional scaling (MDS) plot of the 1000 most variable loci showing that no significant batch effect can be detected, while samples genders are easily discriminated.
Differential methylation analysis
The approach uses multiple metrics and statistics to ensure that different and complementary characteristics are retained in the final ranked list.
Methylation differential values were quantified as: (i)
Similarly, statistical tests (
To ensure robustness of the ranking, the intersection of the statistical and differential approaches was preserved (namely: {
Additional file 2
Table of the DM.
Click here for file
Figure 2
SAM plot
SAM plot. SAM plot depicting the observed
Differential expression analysis
We collected data from 3 datasets in Gene Omnibus Express (GEO,
The expression of mRNAs was monitored by CapitalBio cDNA 22 K long oligo dye-swap microarray, and compared between the two cell lines. Downloaded data were filtered by space- and intensity-dependent normalization (LOWESS), and already summarized as ratio changes for each probe set. No additional pre-processing was performed and fold-change was used with a cut-off 2 to select differentially expressed genes (DEs) in each comparison, leading to 478 DE genes (Additional file
Additional file 3
Differential results for mRNA data.
Click here for file
The 2 miRNA datasets were pre-processed (separately, as based on different versions of the CapitalBio mammalian miRNA array) by summarizing the expression value for each set of probes with the median (3 probes for one miRNA). Quality filtering was achieved by removing empty entries, probes for quality control (non-human) and data with more than 40% NAs. Overall 313 and 545 miRNAs remained in GSE19980 and GSE22378, respectively. After pre-processing,
Additional file 4
DE settings and differential results of miRNA data. List of miRNA targets by experiments. List of miRNA targets by prediction. List of miRNA targets by both.
Click here for file
For DE miRNAs, targets were obtained by searching experimentally validated as well as predicted miRNA target databases, i.e. miRTarBase (release 2.5)
Additional file 5
Parameters used in predicting miRNA targets.
Click here for file
Results and discussion
Classical approaches were first tested to compute the differentially methylated (DM) loci. Although Student
Additional file 6
Bean plot showing β distribution.
Click here for file
Given the intrinsic difficulties in isolating statistically significant differentially methylated loci, due to the numerosity of epigenomic data (curse of dimensionality and multiple hypothesis testing issue), we chose to explore the data with two metrics to combine their diverse advantages, and to support the ranking offered by these measures with two non-parametric statistical tests.
To satisfy the biological rationale that a differential locus should present a variation in β between two phenotypes, we quantified this difference as: (i)
This ranked selection was backed by the ranking obtained from two non-parametric tests, namely Wilcoxon rank-sum test (WRST,
This recommendation combined with our 150 top candidate selection lead to threshold = 0.17 (Additional file
Besides, although literature indicates the advantage of M-value (derived from the logit transformation of β:
Additional file 7
SAM plot (and its code), which exemplifies application to normally distributed data.
Click here for file
Figure 3
Variance-vs-mean plot of β and M
Variance-vs-mean plot of β and M. Panels A, B show the dependence of variance (SD in the upper panels and IQR in the lower panels) on mean values of β. Panels C, D report the same plots for M (logit transformation of β). Both show a bias of the dispersion especially when SD is used as variance indicator, either toward the middle (for β) or toward the 2 extremes (for M). Collectively these plots show that the
The whole DM process led us into the identification of 146 differentially methylated loci, including several corresponding to RNF39 (the most significant one shown in Figure
Figure 4
Methylation state of cg10568066 - the most significantly differential locus annotated to RNF39
Methylation state of cg10568066 - the most significantly differential locus annotated to RNF39. Among the 8 differentially methylated loci annotated to RNF39 (7 hypo- and 1 hyper- methylated), cg10568066 shows the maximum difference as it is hypomethylated by 0.578.
To test whether altered methylation states could also be mirrored and supported in altered genes' expression, we appended to the DM analysis a careful selection of gene expression data at both the transcriptional and post-transcriptional levels from GEO (see Methods). Due to the lack of available blood samples data we turned to hepatic cell lines, implying the assumption that the systemic effects visible in blood mirror events occurring in the disease target organ (liver), a fact that has been observed and confirmed in numerous diseases
The comparison between the DM and DE lists let emerge the covariation of methylomics, transcriptomics, and post-transcriptomics (Figure
Figure 5
Transcriptional and post-transcriptional regulation of DM loci genes
Transcriptional and post-transcriptional regulation of DM loci genes. In panel A, transcriptional (x-axis) and post-transcriptional (y-axis) differential expression (DE, log fold change) related to the genes on the differentially methylated (DM) loci (average β differences, data points' diameters. SULF2 only falls beyond the axes, indicating contribution of all 3 variations (transcriptional, post-transcriptional, methylation) to its final state. Panel B quantifies further such variations.
Figure 6
Transcriptional and post-transcriptional regulation of SULF2
Transcriptional and post-transcriptional regulation of SULF2. The upper panel shows gene expression change of SULF2 (cg 21130926) in the HBV infection model (green: before infection, red: after infection). The lower panel depicts 2 possible mechanisms for the control of SULF2: transcriptional (lower left) and post-transcriptional (lower right).
Epigenomic alterations have recently been discovered as molecular modifications with the potential to stably influence biological systems. Yet, the challenges involved in processing this type of information are numerous, including not only the biological mechanisms triggering and maintaining these modifications, but also the mathematical modelling behind these data, and from there the definition of appropriate methods of analysis. We propose here a combination of approaches to efficiently explore these data and effectively rank a selected number of epigenomic potential causes. In particular, we have been able to highlight the hypomethylation of the transcription factor RNF39 and the controlled expression of SULF2 as two interesting molecular variations related to the HBV vaccine responsiveness.
HBV: Hepatitis B virus; HCC: Hepatocellular carcinoma; DM: Differential methylation; DE: Differential expression; SAM: Significance analysis of microarrays; LIMMA: Linear models for microarray data; WRST: Wilcoxon rank-sum test; FET: Fisher's exact test; MDS: Multiple dimensional scaling.
Competing interests
The authors declare that they have no competing interest.
Authors' contributions
WY conceived the clinical study; WY and YC performed the experiments; CN and YL conceived the bioinformatics study; YL analyzed all data; YL and CN prepared the manuscript. All authors read and approved the final manuscript.
This work is supported by Chinese National "Twelfth Five-Year" Plan for Science & Technology Support (Grant No. 2012BAI03B00); National Natural Science Foundation of China (Grant No. 30960329 and 81273168); Ministry of Science and Technology (Grant no. 2013DFA30790). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
DNA methylation landscapes: provocative insights from epigenomics
DNA methylation profiling in the clinic: applications and challenges
High density DNA methylation array with single CpG site resolution
Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning
Genome-scale DNA methylation maps of pluripotent and differentiated cells
Analysing and interpreting DNA methylation data
Microarray data normalization and transformation
Significance analysis of microarrays applied to the ionizing radiation response
Linear models and empirical bayes methods for assessing differential expression in microarray experiments
Genetic effects have a dominant role on poor responses to infant vaccination to hepatitis B virus
WHO | hepatitis B
NCBI GEO: archive for functional genomics data sets—10 years on
R: a language for data analysis and graphics
Complete pipeline for Infinium®Human Methylation 450 K BeadChip data processing using subset quantile normalization for accurate DNA methylation estimation
Individual comparisons by ranking methods
On the interpretation of
Dynamic changes in the human methylome during differentiation
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository
Genomic analysis of anti-hepatitis B virus (HBV) activity by small interfering RNA and lamivudine in stable HBV-producing cells
Altered expression profiles of microRNAs in a stable hepatitis B virus-expressing cell line
miRTarBase: a database curates experimentally validated microRNA-target interactions
TarBase 6.0: capturing the exponential growth of miRNA targets with experimental support
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are MicroRNA targets
The microRNA.org resource: targets and expression
DNA methylome signature in rheumatoid arthritis
Bioconductor - methylumi
Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysis
An evaluation of analysis pipelines for DNA methylation profiling using the Illumina HumanMethylation450 BeadChip platform
Changes of peripheral A2A adenosine receptors in chronic heart failure and cardiac transplantation
Alteration of adenosine receptors in patients with chronic obstructive pulmonary disease
A2A adenosine receptor overexpression and functionality, as well as TNF-α levels, correlate with motor symptoms in Parkinson's disease
Sulfatase 2 up-regulates glypican 3, promotes fibroblast growth factor signaling, and decreases survival in hepatocellular carcinoma
Heparin-degrading sulfatases in hepatocellular carcinoma: potential roles in pathogenesis and identification of therapeutic targets
Pre-publication history
The pre-publication history for this paper can be accessed here:


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The Disability Studies Reader
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The Fourth Edition of the Disability Studies Reader breaks new ground by emphasizing the global, transgender, homonational, and posthuman conceptions of disability. Including physical disabilities, but exploring issues around pain, mental disability, and invisible disabilities, this edition explores more varieties of bodily and mental experience. New histories of the legal, social, and cultural give a broader picture of disability than ever before.
Now available for the first time in eBook format 978-0-203-07788-7.
Taylor and Francis; Read online
Title: The Disability Studies Reader
Author: Lennard J. Davis
Title: The Disability Studies Reader
Author: Lennard J. Davis
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Deep Six
The Trunk Space, one of downtown Phoenixs top art galleries and performance venues, celebrates its sixth anniversary this month. Ample toasts and huzzahs should be sent the spots way, especially considering the rough and tumble place that the local art scene can be.
The Trunk Space cheers the artists that have decked its halls over the years with the Secret Six, a group exhibition that gives it up to six main players that have made their mark on the space. For participating artist Gregory Sale, the meaning is nearly literal. His interpretive piece, which is hanging outside on one of the buildings walls, includes oversized buttons that declare, "I could be the sound you can't pronounce."
Other artists in this show include clay works by Julian Vandercook, an Arizona sculptor currently living in Himeji, Japan; downtown stalwart Janet de Berge Lange, who created a wall-sized, quilt-style assemblage entitled Girl (its one of our favorite pieces in the exhibit); Doug Oland and his stirring works in oil; Camilla Taylor and her printmaking works on canvas; and art-decathlete Lisa Takata.
Fri., April 2, 6-10 p.m., 2010
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A memory shared byon Mar 19th, 2011.
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