text: CHESTERFIELD, Mo., Aug. 25 /PRNewswire-FirstCall/ -- Reliv International (Nasdaq: RELV) and the Reliv Kalogris Foundation have won 2010 Communitas Awards for excellence in community service and corporate social responsibility. Reliv International won for its "Week of Caring" project in the company-sponsored volunteer project category. Held during National Volunteer Week in April, groups of Reliv employees took part in community service projects at five St. Louis area non-profit organizations, volunteering their time but also money and critical supplies. The Reliv Kalogris Foundation, the company's charitable arm, won in the philanthropy category for the building of its second feeding center/school/community center in Bois Lance de Limonade, Haiti. The center is one of more than two dozen Reliv feeding centers that provide free nutritional supplements to thousands of Haitians every day. "These awards represent great honors for Reliv, our employees and our distributors," said R. Scott Montgomery, chief operating officer of Reliv International and chairman of the Reliv Kalogris Foundation. "The 'Week of Caring' enables and encourages our employees to become engaged with organizations that perform important community services." Montgomery added, "Our distributors generously donate money to support the work of the Reliv Foundation, which provides free nutritional supplements to more than 42,000 individuals daily in 10 countries. Some funds also are used to build feeding centers in poverty-stricken parts of the world, like Haiti." The Communitas Awards program is an international effort to recognize the spirit of communitas – a Latin word referring to people coming together for the good of a community. The Communitas Awards program is an outgrowth of the pro bono recognition program of the Association of Marketing and Communication Professionals (AMCP). About Reliv International


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18:25 18th January 2014
Burnley 1 Sheff Wed 1
Roses games between Burnley and Sheffield Wednesday are rarely predictable affairs.
Just once in the last 15 meetings had the home side won; a sequence that was extended further after Sam Vokes 12th goal of the season was cancelled out by Chris Maguire.
Vokes struck three minutes before the break after the Clarets dominated the opening 45 minutes.
But Maguire earned a precious point for the Owls on the stroke of half time with a close-range effort.
And despite mounting increasing pressure on the Wednesday goal in the second half, the Clarets just could not find a way past the inspired Chris Kirkland in the visitors' goal.
Sean Dyche named an unchanged side to the team that beat Yeovil Town 2-1 at Huish Park.
And the Clarets started well, with Keith Treacy heavily involved against the club he joined on loan last season.
In the 11th minute the winger was a whisker away from opening the scoring as Burnley carved out the first opportunity. Vokes' header fell behind the last man and when strike partner Danny Ings' attempted chip over Kirkland caught a glance off the keeper, Treacy was on hand, only for Glenn Loovens to knee his goalbound effort towards an open net for a corner.
At the other end it took a perfectly timed tackle eight yards from goal from David Jones to deny Kieran Lee a certain goal.
Twenty-one goal Ings was soon in on the act, following his piledriver at Yeovil with another long range effort that Kirkland gloved over.
And as the end-to-end start continued, Connor Wickham's flick header sent Caolan Lavery free, only for the striker to fire across Tom Heaton and wide.
Back came Burnley and from Trippier's throw, superb touch and awareness from Sam Vokes sent Dean Marney clear, only for the midfielder to toe-poke wide as he homed in on Kirkland.
Burnley were now very much in the ascendancy and Treacy's drilled left wing cross somehow evaded everyone to hand Wednesday another let off.
But three minutes before the break, the breakthrough finally arrived, as arney pounced to dispossess Semedo and dink a ball over the top for Vokes to slot confidently underneath the advancing Kirkland.
However, the lead lasted barely a minute as Wednesday levelled before the break.
A deep free kick was headed on and as the Clarets allowed it to bounce from a flick-on, Maguire made no mistake, rifling high into the net inside the six-yard box.
The second half began as a much tighter affair, before a deflected Lavery shot forced Heaton into evasive action just before the hour mark.
But somehow Wednesday survived when Burnley threatened with their first chance of the half in the 65th minute.
Vokes did brilliantly to chase the ball and spin his marker into the turf before cutting the ball back for the inrushing David Jones, whose goalbound effort was brilliantly headed off the line by a retreating Liam Palmer.
As Burnley moved up through the gears, Scott Arfield then saw a glorious chance flash across goal and wide as he took advantage of a defensive slip.
And the game fully opened up, Maguire was booked for a dive in the box as he went past Danny Lafferty in the aim of winning a penalty.
Ashley Barnes was handed a home debut with eight tense minutes remaining, replacing Treacy as Burnley went for the jugular.
But it was strike partner Ings who took centre stage in the dying minutes.
The striker first spurned a glorious chance as he failed to make connection with Trippier's excellent right wing delivery.
Then, in the dying seconds of normal time he slammed a shot off the foot of the post after a long throw fell invitingly.
Seconds later, Ings brought yet another fine save from Kirkland as he spun in the box and went for the far corner.
But the former Liverpool stopper saved his best until last, earning his side a pojnt with a breathtaking last-gasp save from the Clarets top scorer after Barnes chased a lost cause down the left.
Burnley: Heaton, Trippier, Duff, Shackell, Lafferty, Treacy (Barnes 82), Marney, Jones, Arfield, Ings, Vokes.
Subs: Cisak, Long, Stock, Kightly, Stanislas, Edgar.
Booked: Marney 88
Sheffield Wednesday: Kirkland, Palmer, Onyewu, Loovens, Mattock, Lee, Semedo, Maguire (McCabe 90), Maghoma (Nuhiu 68), Wickham, Lavery (Helan 84)
Subs: Martinez, McPhail, Llera, Johnson
Booked: Semedo 64, Wickham 68, Magiure 78, Mattock 81
Referee: Mr Stuart Atwell
Att: 13,735 (1,848)
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text:
Nothing should surprise us within an alleged meritocracy that rewards Jeremy Hunt with promotion to Health Secretary, when by any credible yardstick he should have resigned. Nevertheless it would take some Hunt-like chutzpah for Glenys Stacey to stay in her role as Ofqual boss after the latest developments in the GCSE grades fiasco.
The revelations, via letters first obtained by the TES, that Ofqual itself had put pressure on the Edexcel board to shift the grade boundaries despite the board's reservations is bad enough. That it did so just a few weeks before the summer grades were issued, and subsequently played dumb, is simply appalling.
Stacey has now admitted she would have forced the boundaries to have been changed if Edexcel had not done so, but "pressure"? No sir. Not me sir. Oh, and to make matters even worse, the English GCSEs at the heart of the row are to be remarked in Wales, but not England. Yet.
As many an anxious parent has written to us: children's grades are no game. But, even in games you do not bend or change the rules halfway through, as happened this school year.
Michael Gove has a real opportunity now. He can show that he is not an arrogant ideologue by ordering a regrading, or reveal his true intent by carrying on regardless, even so far as to close schools that are suddenly "failing".
Behind the farce we see again the insidious effect of not just the heinous school league tables, but exam board league tables. When will we wake up and stop messing with our children's lives? For many it is already too late.Reuse content


text:
Life Without the Gloss In New Directors Series
By STEPHEN HOLDEN
Published: March 15, 1991
Movies and television are so inundated with MTV-style images of sexy young bodies springing confidently into action on a rush of pop music that more realistic, alternative visions of youth are increasingly hard to find these days. One place that offers a respite from the depiction of young people as hyper-competitive proto-yuppies or gangsters is the New Directors/New Films series, the annual festival presented by the Film Society of Lincoln Center and the Museum of Modern Art.
The youthful characters who ramble through this year's series, which begins public screenings tonight at the museum's Roy and Niuta Titus Theaters and runs through March 31, are not the sorts one would expect to find in commercials for soft drinks or sneakers. Richard Linklater's "Slacker" looks at a decidedly unglamorous population of college dropouts, eccentrics and neo-beatniks in Austin, Tex. The main characters of Caveh Zahedi and Greg Watkins's "Little Stiff," a black-and-white film that has the drab look and static momentum of early Warhol, are shy, mousy-looking art-school students who talk in ums and ers and can barely screw up the courage to ask each other out on dates.
Philippe Faucon's "Amour," a French film that is on the opening bill today, tracks a group of buxom girls and their skinny, fumbling boyfriends in a working-class suburb of Paris. Obsessed with sex, they mope about the streets and nightspots of their neighborhood coupling and uncoupling. But while there is plenty of lovemaking, there is more anxiety than joy in an activity that is summed up by one girl's comparison of the choosing of boyfriends to shopping for shoes.
Taken together, these movies represent an obvious reaction to the speedy, flesh-peddling ambiance of mass-market movies that pretend to be hip in portraying the young. A rejection of visual flash and high-tech bravura is also a response by up-and-coming film makers and video artists who, on the basis of their early low-budget work, might be called the blank-tape generation. Academic Settings
"There has always been this huge alternative culture, one that the media has never picked up on, of people biding their time and refusing to take their preordained places," observed Mr. Linklater, whose film cost $23,000 to shoot. He said he got the idea for "Slacker" seven years ago "in a flash." The word "slacker," he explained, is a Texas nickname for the type of non-student who clusters around the University of Texas campus eking out a marginal income while living a quasi-collegiate existence.
"A Little Stiff," which also has a university setting, was inspired by Ozu, Brecht and Godard, said Mr. Zahedi, who plays the leading role in the film. Most of the movie, which takes place on the campus of the University of California at Los Angeles, was filmed in medium to long-distance shots. Its detached, documentary air was intentional.
"We didn't want to tell people how to think or to manipulate them in a visual sense," Mr. Zahedi explained.
Richard Pena, the program director of the Film Society of Lincoln Center, said Mr. Faucon, in directing "L'Amour," sought to make a less-exploitative film in the same social stratum as "Sound and Fury," the disturbing fictional study of life in a violence-plagued French high-rise that was shown in the New Directors series last year. The Most Ever
Movies about young people are only a small part of the festival, whose 22 programs will show 36 films from 15 countries. This year, the United States is represented by 6 feature films, 4 featurettes and 3 shorts, the highest number ever in the series, which is celebrating its 20th year. Three films are from France, and 2 each from Italy, Denmark, and the Soviet Union. For the first time, films from Peru and the Faroe Islands are being shown. Notably absent from the list of countries represented is China.
"One of the pleasures of this year's selection is that in several films there is interesting narrative experimentation going on," Mr. Pena said recently. "During the 80's, you saw a return to the embrace of strict narrative forms. The freedom to play with narrative was a great tradition of the 60's, which faded out. Now that freedom seems to be coming back, though the kinds of experimentation are not the same as in the 60's."
Two of the films Mr. Pena pointed to were Katrin Ottarsdottir's "Atlantic Rhapsody: 52 Scenes for Torshavn," from the Faroe Islands, and "Slacker," both of which compile a portrait of a community by examining a long succession of characters.
Silvio Soldini's "Peaceful Air of the West," from Italy, follows around a bunch of discontented middle-class Italians living in contemporary Milan. Though many of the characters are completely unrelated, their paths cross randomly in surprising ways. If the narrative rhythms of the movie recall Michelangelo Antonioni's early 60's films, "Peaceful Air of the West" has none of Mr. Antonioni's romantic despair.
Many characters people another film from Italy, Pupi Avati's "Story of Boys and Girls," a lush ensemble piece that portrays the meeting of two clans from different strata of Italian society at an engagement banquet in the mid-1930's.


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text:
Printed score reports will be available this summer.
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text:
Notre Dame hopes to finally bring back the football echoes
SOUTH BEND, Ind. Maybe you've heard this one before. Around here, they certainly have.
Notre Dame wants its glory back. The Irish hope they are getting closer, and it is only a matter of time before the echoes wake up from a coma. They hope for the days when they can again beat anybody. Even Navy.
Sound familiar? The Irish have hoped before — every season, through this coach or that one. But revival has come in stops and starts. How long has it been since Notre Dame truly was what Notre Dame is supposed to be, record-wise and ratings-wise and aura-wise?
"A long time," coach Brian Kelly was saying at media day
Try 17 years. The Irish have not won a marquee bowl in that span. They have not finished in the top five of the polls; something 31 other schools have accomplished, including 10 of the Pac-12 lodge members. They have endured 28% of their all-time losses, even though Notre Dame has played football since 1887.
Kelly ended his first year in 2010 with four consecutive wins, which turned a shaky honeymoon season into 8-5. An appetizer for the good times to come ("That doesn't fill you up," Kelly mentioned). A booster shot for confidence.
That's the plan, anyway.
Plus, there was death. A student was killed filming practice when winds blew over a tower, and the shadow lasted all season.
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To get the latest sports news from USA TODAY, including game results, columns and features, follow us on Twitter at @USATODAYSports.
So the Irish have to raise the bar on what is good, back to where it sat for generations. And they must hope the news stays on the field.
"That confidence can be eroded if you don't turn that into winning early, you know what I mean?" Kelly said.
We do. Notre Dame, annually eager for renaissance, hasn't been 3-0 since 2002.
"We haven't done what we came here to do," senior guard Trevor Robinson noted. "Nobody really comes here to be 8-5."
The Irish have to decide on a starting quarterback. Dayne Crist and Tommy Rees can hardly be separated by a leprechaun's four-leaf clover, but Kelly will have to pick one soon. The deciding factor? "I'll know it when I see it," he said.
They have to hope wayward receiver Michael Floyd has seen the light. Kelly suspended him after a DUI charge in the spring — not Floyd's first run-in with alcohol — but took him back for the season.
"He could have let me go on my own and whatever happened, happened," Floyd said. "He knows I'm a better person than I showed."
One condition is that Floyd live in a student dorm rather than off-campus as a senior, so this past week, he was moving into Dillon Hall, with everyday Joes who will never run a crossing pattern in their lives.
The Irish must hope the defense continues to improve and the depth gets better. "I'll put my 22 (starters) against anybody else," Kelly said. "But the game's not played with 22."
If all that turns out, does the thunder get shaken down, finally?
"In the past, everyone has said we are going to be better," tackle Zack Martin said, "but I think everyone on the team has really bought in."
Senior linebacker Darius Fleming: "We read about the past. We try not to worry about it too much, but it shows when we look in the locker room and see the trophies.
"Those are the places we want to be."
Fact is, college football — up to its earholes in scandal and bad news — could use a dose of Notre Dame. Not because the Irish are saints among villains; they have problems like everyone else. As Kelly said of Floyd's blemishes, "It's part of who Michael Floyd is for the rest of his life, because of the mistakes he's made … He's got to do the right things every day, and I think he has."
And if Floyd doesn't, Kelly will take the darts about being soft on stars.
But the Irish as contenders evoke brighter traditional days in the sport, love 'em or hate 'em. "This place is different than anywhere else in the country," Crist said. And it always helps when the football team is winning.
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Bend has been named by just about every magazine, from Forbes to US News and World Report, as one of the best places in the world to live, retire, mountain bike, ski, golf and hike. Here, you will have no shortage of active pursuits. We are also blessed with quaint shops, excellent restaurants, countless breweries and an eclectic arts and music scene. Further afield, we can boast world-renowned fly fishing and rock climbing. Surrounded by everything that makes living in a place desirable and rewarding, Tetherow truly is at the heart of it all.
Festival Town USA
When Bend puts on a show, we do it right. Maybe that's why we are home to the Cascade Cycling Classic, Pole Pedal Paddle, BendFilm, the Cyclocross world regional qualifier race, and Spring, Summer, Fall and Winter Festivals. These festivals provide an almost weekly slate of entertainment. Take in the summer's farmers markets or a world famous act at the Les Schwab Amphitheater, and you'll see why Bend continues to be a top travel destination.
Shopping in Bend
From the national retailers that you've come to love to the eclectic and unique shops you'll learn to crave, the Old Mill and Bend's downtown satisfy. Whether you're looking to find that perfect something for a shelf in your home or picking out a gift for a friend, shopping has never been more fun or more convenient.
Food in Bend
In addition to the outstanding options at Tetherow, Bend offers many culinary delights! From fine dining at Ariana, 5 Fusion & Sushi Bar or Zydeco, or the family-friendly atmosphere of the restaurants in the Old Mill, Bend has experienced a culinary renaissance in the past decade. Touted by national magazines as a culinary hotbed, our restaurants pride themselves on sourcing their ingredients locally. Whether at Tetherow or elsewhere, whatever your appetite, Central Oregon has you covered!
Skiing in Bend
With over 500 inches of light, dry snowfall in the nearby mountains each year, it's no wonder the US Ski and Snowboard teams frequent Mt. Bachelor for training. It's not out of the ordinary to share the slopes with world-class skiers and snowboarders. That's not to say mere mortals can't enjoy this winter playground every bit as much as the elite athletes. Terrain ranging from extreme to extremely gentle, 56 kilometers of groomed cross country trails, and world-renowned snow parks make Mt. Bachelor the ideal place for your family to ski and board.
Cycling in Bend
From world-class mountain biking trails right out your back door to endless road biking routes along scenic byways, Bend is a bicyclist's paradise. You will also have easy access to our famous Three Sisters Scenic Bikeway and Urban Trail System. And if you're simply looking for a relaxing tour on your favorite cruiser, you'll be in good company in Bend. Our bike lane system is a model for other communities. And all your favorite breweries have ample bike parking. Just remember to ride responsibly.


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text:
Global transcriptome analysis of two wild relatives of peanut under drought and fungi infection
- First Online:
- Received:
- Accepted:
- Cite this article as:
- Guimarães, P.M., Brasileiro, A.C., Morgante, C.V. et al. BMC Genomics (2012) 13: 387. doi:10.1186/1471-2164-13-387
- Citations
- 5.7k Downloads
Cultivated peanut (Arachis hypogaea) is one of the most widely grown grain legumes in the world, being valued for its high protein and unsaturated oil contents. Worldwide, the major constraints to peanut production are drought and fungal diseases. Wild Arachis species, which are exclusively South American in origin, have high genetic diversity and have been selected during evolution in a range of environments and biotic stresses, constituting a rich source of allele diversity. Arachis stenosperma harbors resistances to a number of pests, including fungal diseases, whilst A. duranensis has shown improved tolerance to water limited stress. In this study, these species were used for the creation of an extensive databank of wild Arachis transcripts under stress which will constitute a rich source for gene discovery and molecular markers development.
Transcriptome analysis of cDNA collections from A. stenosperma challenged with Cercosporidium personatum (Berk. and M.A. Curtis) Deighton, and A. duranensis submitted to gradual water limited stress was conducted using 454 GS FLX Titanium generating a total of 7.4 x 105 raw sequence reads covering 211 Mbp of both genomes. High quality reads were assembled to 7,723 contigs for A. stenosperma and 12,792 for A. duranensis and functional annotation indicated that 95% of the contigs in both species could be appointed to GO annotation categories. A number of transcription factors families and defense related genes were identified in both species. Additionally, the expression of five A. stenosperma Resistance Gene Analogs (RGAs) and four retrotransposon (FIDEL-related) sequences were analyzed by qRT-PCR. This data set was used to design a total of 2,325 EST-SSRs, of which a subset of 584 amplified in both species and 214 were shown to be polymorphic using ePCR.
This study comprises one of the largest unigene dataset for wild Arachis species and will help to elucidate genes involved in responses to biological processes such as fungal diseases and water limited stress. Moreover, it will also facilitate basic and applied research on the genetics of peanut through the development of new molecular markers and the study of adaptive variation across the genus.
Legumes are an important source of protein for humans and livestock. Cultivated peanut (Arachis hypogaea) is one of the most widely grown grain legumes in the world. It is widely cultivated mainly in Asia, Africa and the Americas, and is valued for its high protein and unsaturated oil contents [1, 2]. Worldwide, the major constraints to peanut production are drought and fungal diseases including Early (ELS) and Late Leaf Spots (LLS), the latter caused by Cercosporidium personatum[3, 4, 5].
Wild Arachis species, which are exclusively South American in origin, have high genetic diversity and have been selected during evolution in a range of environments and biotic stresses, and constitute a rich source of allele diversity [6, 7, 8]. The species A. stenosperma harbors resistances to a number of pests, including the root-knot nematode Meloidogyne spp. [9] and fungal diseases [10, 11], whilst A. duranensis is originated from regions with relatively low rainfall [12]. These wild relatives are a rich source of new alleles for peanut improvement as they have sufficient polymorphism for their genetic characterization, and the tracking of genome segments that confer these resistances during introgression into cultivated peanut [13, 14, 15]. Both species have been exploited as a resource for gene discovery, interpretation of genomic sequences and marker development [11, 15, 16, 17, 18, 19] and are also parents of a recently developed RIL (Recombinant Inbred Line) diploid mapping population.
In recent years, a relatively large number of EST sequences have been made available in the National Center for Biotechnology Information (NCBI) public database for A. hypogaea (151,352). However, fewer resources exist for A. duranensis (35,292) and A. stenosperma (6,264). In addition, a whole genome sequencing project for peanut (tetraploid) remains a challenge, in part due to the size of the genomes compared to model plants, even for the diploid species (A. duranensis 1,260 Mbp vs. 115 Mbp in Arabidopsis thaliana), but even more because of the high repetitive DNA content [20, 21].
Functional genomics studies, using microarrays and subtractive libraries (SSH), identified genes potentially associated to stress responses to C. personatum and drought stress in Arachis spp. [3, 4, 22, 23]. However, to our knowledge, no massal transcriptome analysis in stressed wild Arachis is available.
Increased transcriptome sequence resources should facilitate basic and applied research on genetics, contribute to the development of molecular markers, facilitate comparative genomics and aid in the study of adaptive variation across the genus. In addition, transcriptome data can assist on the elucidation of genes involved in biological processes, such as defense responses to biotic and abiotic stress, which Transcription Factors (TFs) are notably associated [24, 25], and have hardly been studied in wild Arachis.
Although it is still a challenge to assemble a new whole complex genome using Next-Generation Sequencing technologies (NGS) (454/Illumina), the smaller size and reduced repetitive content of the transcriptome together with increased coverage facilitates the de novo transcriptome assembly using these technologies [26]. Next-generation sequencing technologies have facilitated large scale generation of ESTs cost-effectively, and allowed the whole transcriptome analysis of a number of smaller scale legume crops such as chickpea, lentil, mungbean and pigeonpea [27, 28, 29, 30]. Moreover, deep sequencing has enabled the identification of new transcripts not present in previous model crops EST collections, such as Arabidopsis[31] and rice [32], and the massive identification of molecular markers such as SNPs (Single Nucleotide Polymorphisms) and SSRs (Simple Sequence Repeats) [33, 34]. In addition, EST-SSRs being genic in origin, frequently display a high degree of transferability between related species.
In the present study 743,232 sequence reads were produced using Roche/454 GS FLX Titanium, generating a total of 17,912 unigenes for A. stenosperma and 21,714 for A. duranensis submitted to infection with C. personatum and gradual water limited stress, respectively. Contigs derived from these reads were annotated into functional categories separately for each species, and the expression of five Arachis RGAs (Resistance Gene Analogs) and four sequences related to the only fully-characterized Arachis retrotransposon (FIDEL) [20] further analyzed by qRT-PCR (quantitative reverse transcription-PCR). This database was also used to design a set of 214 EST-SSRs primers which showed polymorphism via electronic PCR (ePCR) between the two species studied.
The genomic resources developed in this study can, in association with other tools already developed for wild Arachis, contribute to accelerate genetics and breeding of peanut and contribute particularly for the elucidation of genes involved in responses to important biological processes such as fungal diseases and water limited stress in peanut and other legumes.
EST sequencing and assembly
Total number and length of 454 GS FLX Titanium reads for each library
Number of total reads
Total length (bp)
Number of high quality reads
High quality length (bp)
A. stenosperma
A. duranensis
Total number of unigenes and genome coverage for each species
A. stenosperma
A. duranensis
Average number for both species
Total number in both species
Number of high quality reads
Number of reads after clustering
238, 456
*Percentage of accepted reads (%)
Number of unigenes
Number of contigs
Average contigs length (bp)
Average number of reads per contig
Contig genome coverage (Mbp)
The genome coverage of contigs from A. stenosperma was 8.18 Mbp and A. duranensis 10.72 Mbp (Table 2), which make just under 1% of the estimated 1,260 Mbp size of a typical diploid Arachis species [35]. Sequence data from this study can be found for each species in the Sequence Read Archive (SRA) at the NCBI (A. duranensis - SRA047273.1; A. stenosperma - SRA047258.1). The derived contigs for each species and their most significant match against the nr database of GenBank (E value < e-7) is available in additional files (Additional files 1, 2) and at NCBI in Transcriptome Shotgun Assembly (TSA) (A. duranensis - JR332677 -JR344253 and A. stenosperma - JR326556 - JR332676).
Sequence annotation and gene ontology
Only the high confidence consensus sequences (contigs) of A. stenosperma and A. duranensis were compared against the NCBI non-redundant protein sequence database (nr) for each species using BLASTX [36] in order to annotate known proteins/genes (Additional files 1, 2). A relatively high rate of contigs, 52.3% from A. stenosperma and 58.5% from A. duranensis, could be assigned to putative orthologs of genes involved in various pathways and cellular processes, when compared to other legumes without a completely annotated reference genome sequence [27, 29]. Over 27% of the overall transcripts in both, A. stenosperma and A. duranensis showed homology in BLASTX to 14 legume species (Additional files 1 and 2). From these, over 60% of A. stenosperma and A. duranensis transcripts showed homology to Glycine max, followed by Medicago spp. (18%). Only 2.9% of A. stenosperma transcripts showed homology to A. hypogaea, whilst 6.5% showed homology to A. duranensis. This data reflects the greater number of ESTs available for these two legumes in comparison with Arachis spp. and also the closeness of A. duranensis to the cultivated tetraploid A. hypogaea[37].
The assignments made to the molecular function ontology was very similar for both species (Figure 3), with a large proportion of the sequences in catalytic (12–15%) and binding activities (14–17%), whilst under the biological process ontology a large proportion fell into metabolic process (15–17%) and cellular process (13%). Additionally, in A. stenosperma, including transcripts from fungi inoculated leaves, 68 sequences were identified in the GO subcategory response to stimulus which included peroxidases, catalases, chitinases, glycosinases and serine/threonine kinases, whilst in A. duranensis, including transcripts from leaves and roots submitted to water limited stress, 126 sequences were in this category with highlight to those sequences related to osmotic stress and water deprivation (Figure 3).
Transcription factors
Expression profile of RGAs and FIDEL
NBS-LRR and FIDEL sequences used for expression analysis using qRT-PCR
Gene/Sequence abbreviation
Primer sequence Forward/Reverse
Amplicon size (bp)
PCR efficiency (%)
Regression coefficient R2
Retroelements constitute the major part of repetitive DNA of a number of animal and plant genomes [43]. The long terminal repeat (LTR) retrotransposon-FIDEL constitutes a significant part of Arachis tetraploid and diploid genomes [20]. FIDEL-related sequences were found to be expressed in both species studied with a surprisingly high frequency. For A. duranensis, 0.23% of the high quality sequence reads, and 37 of the 12,792 (0.29%) contigs were FIDEL or FIDEL-related. For A. stenosperma, 1.3% of the high quality sequence reads, and 87 of the 7,465 (1.16%) contigs were FIDEL or FIDEL-related. In silico analysis indicated that most of these contigs were up regulated in response to the biotic/abiotic stresses. Four FIDEL-related contigs (Table 3) were chosen for analysis by qRT-PCR using cDNA from A. stenosperma leaves and A. duranensis roots, as template, and actin or 60S as reference genes.
We found that, with the exception of FIDEL274 in A. stenosperma/C. personatum samples, all representatives of this retroelement showed an increased expression in both species under biotic and abiotic stress (Table 3; Figure 5B). It is also interesting to note that, the levels of induction of the four FIDEL sequences were slightly higher in A. duranensis submitted to water limited stress than in A. stenosperma under fungi inoculation (Figure 5). The sequence FIDEL 412 showed the highest difference in expression levels between stressed and non-stressed plants with 1.74-fold expression ratio (Figure 5B).
SSR identification
Frequency and repeat type of SSRs inA. duranensisandA. stenospermatranscripts
SSR mining
A. duranensis
A. stenosperma
Total sequences analyzed
Total sequences with repeats
Total primers designed
SSR in repeat types
SSR polymorphism
Amplified in other parent*
Polymorphic §
Percentage of polymorphic primers
Transcriptome sequences are a valuable resource, especially for species without a completely sequenced genome, such as peanut. They accelerate gene discovery, provide an asset for molecular markers development and allow expression analysis and evolutionary genome dynamics studies. In the present study, Next Generation Sequencing (NGS) enabled the generation of large numbers of sequence reads in a rapid and cost-effective manner, and enabled the development of genomic resources for the exploitation of the stress resistances harbored by two wild diploid relatives of peanut.
Some recent studies have indicated that short reads from 454 GS 20 and GS FLX can effectively be used to characterize gene regions in a number of less studied species, including some tropical legumes [26, 28, 29, 30, 45, 46]. In the present study, the average read length for both species was of 280 bp, which allowed estimated genome coverage of up to 163 Mbp of high quality reads for both diploid Arachis genomes studied in a single sequencing run. In comparison with other studies in legumes, a relatively small number of singletons were produced (8,922 for A. duranensis and 10,189 for A. stenosperma), furthermore the average length and number of reads per contig assembled was comparatively high (475.5 bp and 26 reads/contig) (Table 2) [27, 29, 47]. This may in part be due to very stringent quality and assembly parameters used, which also may partly explain that only 5% of the contigs produced in this study (1,012) failed to show significant functional annotation.
The lack of a complete sequenced and annotated reference genome makes it very difficult to estimate the genome coverage obtained in this study for both species analyzed. However, if we take as comparison other diploid legume genomes which have already been completely sequenced and assume the same number of genes, as for Medicago truncatula (38,835) and Lotus japonicus (42,395), we could suggest that up to 54% of the A. duranensis (21,714) and 44% of A. stenosperma (17,912) unigenes were covered in our work. However, it is also important to be aware that more than one contig or singleton can be originated from a single gene due to either non-overlapping sequence reads or high levels of sequence error in a single read [27].
Transcription factors (TFs) are of special interest due to their role in controlling plant developmental processes and responses to environmental conditions, including functions of key importance to agronomic performance [24]. They have an essential role in the signal transduction networks that leads from the perception of stress signals to the expression of stress-responsive genes, and, as opposed to most structural genes, tend to control multiple pathway steps within a transcriptional cascade [25]. Therefore, TFs are expected to be excellent candidates for modifying complex traits in crop plants, with TF-based technologies likely to be a prominent part of the next generation of successful biotechnology crops [48, 49]. In the present study, 1% of the transcripts were identified as transcription factors (TFs). Their overall distribution among the various known TF protein families was compatible with previous studies in other legumes such as soybean, chickpea, pigeonpea and cultivated peanut [4, 28, 30, 50, 51], with bZIP, MYB, NAC, bHLH, AP2-EREBP and WRKY highly represented in both A. duranensis and A. stenosperma transcripts.
The most expressed TF family was the basic leucine zipper (bZIP)-type TF protein, which comprise regulators of many central developmental and physiological processes and abiotic and biotic stress responses [52]. Among other reports, this TF has been associated with water deficit-response in the relatively drought resistant tepary bean (Phaseolus acutifolius)[53] and to abscisic acid (ABA)-regulated gene expression required for the dehydration-response in Arabidopsis[54]. Likewise, this TF family was the most expressed in A. duranensis plants subjected to gradual water limited stress (18%), suggesting a role of this family in this relatively drought tolerant species. The bZIP TF family was also the most expressed TF in A. stenosperma leaves subjected to C. personatum (18%), and has already been described as involved in defense response to other host-fungi interactions, such as to the stripe rust via the ethylene/methyl jasmonate -dependent signal transduction pathways in wheat [55], and to regulate the expression of some stress-responsive genes such as the PR-1 and Glutathione S-Transferase in Arabidopsis[56].
The second most highly expressed TF family in drought imposed A. duranensis plants (12%) and fungi infected A. stenosperma leaves (14%) was the MYB family, which has been described to act through the ABA signaling cascade to regulate stomatal movement and therefore water loss regulation, and disease resistance in Arabidopsis and rice [57, 58]. Likewise, the plant specific NAC transcription family was showed to be highly expressed in A. duranensis (10%) and to a lesser extent in A. stenosperma (2%). NAC proteins function has been previously described in potato and Brassica napus under fungal infection [59, 60] and to significantly increase drought tolerance in soybean and chickpea [61, 62].
Dehydration-responsive element binding (DREB) proteins a subgroup of the AP2/EREBP, have an important role in plant response and adaptation to abiotic stresses [63]. In this study, they constituted 7% of the TFs in A. duranensis plants subjected to water limited stress. A previous study with transgenic peanut plants over expressing DREB1A showed that the changes in the antioxidative machinery in these transgenic plants under water-limiting conditions played no causative role in improved transpiration efficiency [5, 64, 65]. Nonetheless, different DREB homologues have shown to play different roles in increasing tolerance to cold, salt and drought in different plant species, and have been extensively studied in Arabidopsis, rice and soybean being correlated to increased dehydration tolerance in these species [66, 67, 68, 69]. An additional consideration is that recent studies indicate that function of central regulators as NAC, WRKY, and zinc finger proteins may be modulated by mechanisms such as small RNA (miRNA)-mediated posttranscriptional silencing, reactive oxygen species signaling and epigenetic processes such as DNA methylation and posttranslational modifications of histones [70]. This suggests that a more comprehensive elucidation of the role and dynamics of drought and defense responsive TFs in plants may be required.
Retroelements, particularly the long terminal repeat (LTR) retrotransposons, constitute the major part of repetitive DNA of plant genomes. Some of these elements seem to be constitutively expressed and others are silent and can be activated upon certain stress signals such as tissue culture, ionizing irradiation, wounding or poliploidization. As a matter of fact, data from the whole genome sequencing of several eukaryotes strongly suggests that, far from being circumstantial, the activity of transposable elements plays an extremely important role in the plasticity and regulation of host gene functions [71]. The mechanisms of how stress induces the activity of an element are not completely clarified, but it has been shown that most expression features of Tnt1, a Solanaceae retrotransposon, can be deduced from the structure of its regulatory regions, located in the LTR that contains several cis-acting elements, which are similar to well characterized motifs involved in activation of defense genes, whilst the Tnt1A G-box-like sequence is related to the typical ABA-responsive (ABRE) sequences and is identical to the MYC recognition sequence present in many drought-inducible genes [71, 72].
In the present study, many transcripts from both species were identified as having similarity to retroelements. Therefore, we studied in more detail FIDEL, the only fully characterized Ty3-gypsy retrotransposon described in allotetraploid peanut (A. hypogaea) and its putative diploid ancestors A. duranensis (A genome) and A. ipaënsis (B genome) [20]. Using qRT-PCR analysis, we observed that FIDEL showed an increased expression ratio in both, A. duranensis roots subjected to gradual water limited stress and A. stenosperma leaves inoculated with fungus, when compared to non-challenged plants. In tobacco and other Solanaceae, drought stress and fungi infection have been described as triggering independent mechanisms of plant defense response and activation of transcription factors and retroelements [71, 73]. In our study, we observed that both biotic and abiotic stresses induced FIDEL or FIDEL-related sequences. However, if the induction of FIDEL represents an activation of some specific FIDEL sequences, FIDEL harboring regions or some more specific response is not known.
Plants, in response to pathogen effectors, have co-evolved specific cytoplasm resistance R protein receptors which recognize individual pathogen effector molecular signatures and activate a second line of defense known as effector-triggered immunity (ETI) [74], also previously known as gene-for-gene or race-specific resistance. In contrast to non-specific response (PAMP-triggered immunity-PTI), which will occur in all members of a particular plant species, ETI operates at the intra-specific level, with resistant genotypes possessing the necessary R gene allele [75]. Conservation of motifs within R genes, such as those present within nucleotide-binding site leucine rich repeat domains, have facilitated their characterization in diverse plant taxa. Putative R genes or Resistance Gene Analogs (RGAs) are commonly clustered, as a result of duplication events occurring under diversifying selection. In Arachis, a previous investigation on RGAs content in a number of wild species [41] showed that from the 78 NBS sequences identified, most fall within legume-specific clades, some of which appear to have undergone extensive copy number expansions. In the present study, all five RGA sequences showed an increase on expression under C. personatum inoculation, when compared to the basal expression in the control samples. This was hardly unexpected, as proteins encoded by disease resistance (R) genes, are mostly constitutively expressed in resistant genotypes, mediating specific molecular recognition of pathogenic microorganisms and triggering signaling cascades that activate defense reactions [76, 77]. A broader characterization of the transcriptional response of a suite of defense genes following stimulation of these R-genes, (i.e. kinases, peroxidases, transcription factors, NPR1) [78], and the defense pathways that they trigger is being conducted via Illumina deep sequencing. This will allow a better understanding of their contribution to the overall resistance response of A. stenosperma to C. personatum.
The transcriptome databank produced in this study enabled the development of 2325 SSR primer pairs of which 214 showed to be polymorphic between the two species. These new markers will enrich the current reference AA diploid Arachis map [19] and other Arachis tetraploid maps under construction. In addition, these EST-SSRs markers exhibit potential advantages when compared to SSRs located in non-transcribed regions due to generally more consistent efficiency of amplification, and enhanced cross-species transferability [27].
The development of new SSRs is of special interest in Arachis because these are still the markers of choice in this genus, due to the difficulties in the application of SNPs markers on the cultivated tetraploid species. Therefore, these new markers will contribute to enrich existing genetic maps, generate more informative genetic and genomic tools and enable the identification of orthologous genes through genome synteny analysis [15].
The use of NGS for transcriptome sequencing of species without a complete reference genome is an effective approach for gene discovery and identification of transcripts involved in specific biological processes. The present work constitutes the largest unigene dataset for A. stenosperma and the second for A. duranensis, providing an insight into genomic architecture of these species and also creating a scaffold of transcribed sequences which will help to elucidate genes involved in biological processes such as fungi and drought- related response genes.
Plant material and library construction
Seeds of A. stenosperma (V10309) and A. duranensis (K7988) were obtained from the Brazilian Arachis Germplasm Collection, and maintained at Embrapa Genetic Resources and Biotechnology (Brasilia–DF, Brazil). For fungi bioassays, two month old plants of A. stenosperma were inoculated with a 50,000 spores⁄ml suspension in 0.5% Tween 20. Plant leaves were collected at 24, 48 and 72 hours after inoculation (HAI) and from non-inoculated controls, as described in our previous work [42], and immediately frozen on liquid nitrogen for RNA extraction.
For gradual water limited stress experiments, A. duranensis three months-old plants were equally divided into two groups of 33 individuals each: one group was subjected to a gradual water limited stress (STR), whilst the control group (CTR) was kept at approximately 70% of field capacity. Daily individual transpiration rate of STR and CTR plants was estimated gravimetrically and no more than 10 g of water loss per day was allowed in STR plants. Normalized Transpiration Ratio (NTR) was calculated between individual transpiration of STR and the mean transpiration of CTR plants, essentially as described by [79]. Leaves and roots were collected at distinct stages of the progressive water deficit (decreasing NTRs: 0.76; 0.73; 0.57; 0.43 and 0.40) and immediately frozen on liquid nitrogen to proceed RNA extraction.
Total RNA was extracted from 250 mg of plant material as previously described [42]. RNA integrity was checked by gel electrophoresis and quantified using Nanodrop ND-1000 (Thermo Scientific, Waltham, USA). To construct four bulked libraries, equal amounts of total RNA for inoculated A. stenosperma (leaves collected at 24, 48 and 72 HAI) and stressed A. duranensis (leaves and roots from all NTR points) were pooled separately from their respective non-treated controls and used for mRNA isolation. For the cDNA libraries construction and sequencing, services of CD-Genomics (http://www.cd-genomics.com) were used employing the Creator SMART cDNA library construction kit (Clontech Laboratories, California, USA) and Roche 454 GS-FLX System with Titanium chemistry.
Sequence processing and assembly
Similarity search and functional annotation
Functional annotation of the cluster consensi was performed by sequence similarity searches using BLASTX program [36] against NCBI's non-redundant sequence database. InterProScan [38] was employed to perform protein domain and motif searches. Gene ontology (GO) terms were assigned by Blast2GO [82].
For the identification of NBS encoding genes in A. stenosperma, predicted Arabidopsis NBS containing proteins identified as described in [41] were used as a BLAST database against which, all A. stenosperma contigs were used as query sequences in a BLASTX search. BLAST detected similarities were considered significant with E-values of 1e-7 or less (Additional file 3). Similarly, predicted FIDEL sequences identified in previous studies [20] were used as a BLAST database against which all A. stenosperma and A. duranensis contigs were used as query sequences in a BLASTX search (E value < 1e-7).
For the identification of TF families represented in this study, from the functional BLASTX annotation (value < 1e-7), all putative TF genes from both Arachis species were selected and, classified according to their respective TF family using the Plant TF database [39].
SSRs identification
The program Mreps [83] was employed for the identification of simple sequence repeat (SSR) along the sequences. The parameters were set to identify perfect di- to hexa-nucleotide repeats with a minimum length of 12 bases. A series of custom-made PERL scripts were created to processes the potential SSR loci and to create flanking primers, based on Primer3 [84]. Electronic PCR was carried out using the PrimerMatch package [85] for the identification of primers amplifying in both species, and from these, the polymorphic set between A. duranensis and A. stenosperma.
Expression analysis by qRT-PCR
Plant materials were obtained in new independent experiments carried out as described above.
After isolation and purification, total RNA of four samples (A.stenosperma leaves inoculated with C. personatum and A. duranensis roots stressed by water limitation and their respective non-treated controls) was digested with DNase (TURBO DNA-free™, Ambion, USA) and reverse-transcribed using SuperScript™ II RT and Anchored Oligo(dT)20 primer (Invitrogen, Carlsbad, CA, USA), as previously described [42]. For qRT-PCR, the Platinum® SYBR® Green qPCR Super Mix-UDG w/ROX kit (Invitrogen, Carlsbad, CA, USA) was used according to manufacturer's recommendations on a ABI 7300 Real-Time PCR System (Applied Biosystem Foster City, CA, USA). Two biological replicates for each of four samples were used for real-time PCR analysis, with each replicate representing a pool of five plants. Reactions were carried out using three technical replicates for each sample. Specific primer pairs were designed for five RGAs and four Fidel – related sequences (Table 3) with Primer3Plus software [84] and qRT-PCR cycling conditions were carried out with a final dissociation curve step, using previously described parameters [42]. Normalization of expression was performed using as references the 60S gene for A. stenosperma and the actin gene for A. duranensis samples [42]. All calculations for relative quantification, such as amplification efficiencies, correlation coefficients R2 values and relative expression profile (comparative Ct method) were performed using 7500 v.2.0.4 software (Applied Biosystem, Foster City, CA, USA).
This work was funded by the host institutions and Fundação de Apoio à Pesquisa do Distrito Federal (FAP/ DF) and The National Council for Scientific and Technological Development (CNPq), Brazil.
Supplementary material
Copyright information
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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It's FRIDAY!!!
Happy Friday ladies! What's on the agenda for the weekend?!
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$2 tees??? WHAT?!?!?!
YAY!!!!!!!! We don't have anything super exciting happening this weekend - we move next weekend so PACKING PACKING PACKING!
I am working on my garage sale stuff online, my hubby is working on the area where we are moving our hot tub to, and I will be making more homemade sensory toys, rattles and finger puppets for Autumn.
Happy Friday to you too! We are just relaxing and we have my daughters 1 year photo shoot on sunday!
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I'm a happily married mother to 3! When I'm not online I enjoy reading, watching movies with my children, catching tv shows with my husband, spending time with extended family and shopping! I also advocate for my special needs boys.


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ED Magedson – Founder
Ripoff Report
Family KennelsFort Langley, British Columbia United States of America
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This report was posted on Ripoff Report on 01/17/2013 05:04 PM and is a permanent record located here: http://www.ripoffreport.com/reports/family-kennels/fort-langley-british-columbia-/family-kennels-breeder-marie-lawlor-takes-no-responsibility-for-sale-of-dog-with-life-t-998777. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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#1 Consumer Suggestion
Large Seizure of dogs from this kennel widely reported
SUBMITTED: Wednesday, February 10, 2016POSTED: Wednesday, February 10, 2016
There has been a seizure of dogs from this kennel, 66dogs in dreadful condition, on February 4th 2016, with the SPCA about to press criminal charges. It is being widely report in the news, in reports such as this one.
I think it's clear that everything the original report says is true, and much more.
Please research the breeder of your dogs carefully.
Are you an owner, employee or ex-employee with either negative or positive information about the company or individual, or can you provide "insider information" on this company?
#2 Author of original report
What a Tangled Web We Weave When First We Practice To Deceive!
SUBMITTED: Friday, November 22, 2013POSTED: Friday, November 22, 2013
Perhaps because it has been some time since I exposed your unfair practices as a breeder, let me refresh your memory.
1. You, Marie Lawlor, of Family Kennels, breeder of Bernese Mountain dogs, and as agent for your daughter, Traci, of Six Angels Kennels, advertised and sold me a Wheaten Terrier in the amount of $1,400.00 on July 29, 2012. (Please see my initial report of January 17th, 2013.)
2. You brought out the parents and other siblings of my pup (6 or so in number) and guess what-- they were ALL WHEATENS.
3. All Vet reports, as stated in my first report, were sent to Traci, your daughter, of Six Angels Kennel, by registered mail. It was signed for when received. They would appear to have some problems with memory loss as well. As a footnote to this paragraph, it seems there have been additional complaints against Six Angels Kennel.
4. In your rebuttal you state "you feel I must set the record straight". WHAT A JOKE! Perhaps you should start by telling the truth and accepting your responsibility as a breeder of Bernese Mountain dogs or as agent for your daughter in the sale of Wheatens.
5. Zoe's Vet and hospital bills are now over $20,000—a choice I made because I love and care for her. You and your daughter should be aware of the possibility of a gene present in one or more of the breeding dogs that could cause pain, suffering and death to an innocent dog as well as heartbreak to unsuspecting buyers.
The only parties who are making UNFAIR AND UNTRUTHFUL CLAIMS are you and your daughter.
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#3 REBUTTAL Owner of company
Unfair and untruthful claims made against people.
SUBMITTED: Friday, November 08, 2013POSTED: Friday, November 08, 2013
I am the owner of Family Kennels, we sell Bernese Mountain dogs and have a excellent guarantee. We have nothing to do with Six Angels Kennel. Since my kennel was brought up in this report, I asked Six Angels Kennel owners if they had any vet reports to substanciate the claim. Since they did not and were smeared by these alligations I feel I must write to set the record straight. All money returns must be varified by Veterinarian reports that can be backed up by the Kennel Vet to make sure they are accurate. Once this this done and all reports are varified refunds are automatic.
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Today, I was having some rare good sex with my husband, when he suddenly said "I'm fuckin' BORED," and pulled out so he could go play his video games. FML
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By Anonymous - / Saturday 22 November 2014 18:18 / Ireland - Kilkenny
Today, less than a day after my cranky downstairs neighbor passed away, I woke up to banging sounds against his apartment ceiling, like the ones he used to make whenever I walked around during the night. I'm shitting myself in fear. FML
By mdsfkljsfsdrewr - / Tuesday 3 June 2014 19:01 / Lebanon - Beirut
Today, it's the last day of my sign language class. At the end of the class, my teacher surprised us by speaking for the first time, also surprising everyone that she wasn't actually deaf. It wouldn't have been so bad had I not just given someone an answer to the test, thinking she couldn't hear me. FML
By booboo300 - / Thursday 3 April 2014 22:16 / Canada - Kanata
Today, I received a letter from the state saying my 14-year-old daughter is now legally recognized as a male. I have no idea what happened. FML
By Anonymous / Tuesday 10 January 2012 17:43 / India


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My neighbour Vera Collings, known to many as Veronica, who has died at the age of 87, was a widely-read radical who loved to travel and delighted in a wide circle of friends and her much-loved cat, Fremsley.
Officially called Vera Patricia, she did not like either of the names given to her by her parents, Grace, a costume-maker at the Playhouse theatre in London, and Tom, a civil servant. Many people called her Pat, but others called her Veronica, a name she said she had been given by the theatre critic Kenneth Tynan, because he said she looked like the actor Veronica Lake.
Having boarded at a convent school outside Oxford and attended Milham Ford girls' grammar school, she was among the earliest generation of women to study at Oxford, going to St Anne's. She had a lively mind and an intellectual curiosity which stayed with her until the end.
As a young woman she had a great sense of adventure. She worked for the New Statesman and then lived in France for a few years teaching English. In the 1960s she moved to New York to take up a job with a publishing company and enjoyed rubbing shoulders with stars and musicians in Greenwich Village. She did not confine her love of literature to English, but read novels in French and Spanish. Back in Oxford, her house was filled with books. As well as working in publishing, she taught English to foreign students until she was 75. She never married – a fact that she often regretted.
In the last year of her life her body started to fail her. She was in and out of hospital and nursing homes, but with typical stubbornness clung determinedly to the idea that she would be able to go back home. She would have been delighted to have left the world around the same time as Tony Benn, with whom she shared a radical perspective.
It was characteristic of Veronica that when I was finding her things for one emergency admission to hospital she needed poetry put in with her toothbrush and nightie. When I hurriedly grabbed something from her shelves, it was Philip Larkin. "You must be joking," she managed to gasp. "Larkin? In hospital? Get me Keats or Heaney, please, but no Larkin."


text:
Unspoken Tales
Afternoon came and gone, and the sun was already setting. The two were still shuffling through the thick snowy forest. "Oh well, sleep time," Elise stated in the middle of the silence between them. "You must be tired."
"We can keep on going, I don't mind," said Letto, pointing ahead.
"It's going to be a very long road," Elise said. She took off her gloves and started digging a little trench. "You need a trench also?"
"Sure, I'll start the fire then," Letto replied.
Elise was in her little ditch while Letto was close to the fire to grabas much warmth as he can. "Hey, aren't you going to be near the fire to get warm?"
"I already said I cannot feel heat," declared Elise.
"Ah, sorry," answered Letto quietly. He gave a small smirk. I guess she doesn't have to worry about those small details. Elise is pretty lucky...
"It's terrible."
Elise got out of her trench and sat on the opposite side of where Letto sat. "It's terrible to not feel warmth or cold. It's a curse."
"A curse?" questioned Letto. "I don't see how..."
"To not feel pain, not even my breathe or the snow in my face," mumbled Elise. "What is hot, what is cold?"
The two were silent and the only sound came from the crackling fire.
"Why do you need to find the Flora de Ceptcion?" asked Elise, quickly switching topics.
"Why? I guess we are kind of stuck in the same hole," chuckled Letto. "I believe the flower can cure almost anything, so I'm going to find it to cure my curse. What about you? Are you also finding the Deception Flower?"
"I guess I am," replied Elise, playing with the snow around her. "I don't know myself, but if you want to find it, I'll help you find it, Mr. Letto!"
Letto started laughing and then opened his backpack and took out a piece of bread. He broke it into two and handed one half to Elise. "This is the last piece of bread I have, Elise," said Letto. "Eat it up and we'll keep on searching for the Deception Flower together!"
Elise nodded happily. She nibbled the bread and smiled. "I am glad I can be of help. There isn't a lot of travelers up these mountains, you know. And I might be a bit petite, being a girl, but I really want to help anyone I can."
"You remind me a lot of my daughter," said Letto with his mouth full. After chewing, he continued. "Whenever I was out working, she would always help clean the house and help travelers whoever passed our town. She's around the same age as you, I think."
"Oh, that's interesting to hear."
"Haha, yeah. I first didn't want to start traveling because I was worried about her, but she told me to keep going. She wanted me to get better, was all she cared. Once I get this wretched curse off my back, I am going back to my girl and stay with her without worried if I know someone is lying or not, haha!"
Letto laughed and laughed, but quietly chuckled after seeing Elise staring into the fire. "What about you? I don't think your mother would want her daughter to be running around in the cold."
"Don't worry about her. She won't worry about me. She was scared when I was born and frightened when I couldn't feel pain. So, she left me."
"Scared? Frightened?"
"Aren't you afraid? I have red eyes, white hair, and I cannot feel pain," said Elise.
Letto was about to speak up after a few seconds, but was interrupted by Elise. "Oh well!" she giggled. "Get a good rest, I shall get some sleep first! Good night!"
She slid back into her trench and was sound asleep. Her loaf of bread was held between her small hands.


text: 'Kill me, then, Voldemort! You will not win, you cannot win! The wand will never, ever be yours–':? 83,023pages on this wiki Add New Page Edit History Rename Talk0 Share grindelwald Retrieved from "http://harrypotter.answers.wikia.com/wiki/%27Kill_me,_then,_Voldemort!_You_will_not_win,_you_cannot_win!_The_wand_will_never,_ever_be_yours%E2%80%93%27:?oldid=182876" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Answered questions


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Econo Lodge Inn And Suites
Select your stay dates to check availability at Econo Lodge Inn And Suites
Econo Lodge Inn And Suites
About the Hotel
Property Location
With a stay at Econo Lodge Inn And Suites in Bridge City, you'll be within the region of London Bridge Park and Shangri La Botanical Gardens and Nature Center. This hotel is within the region of Heritage House Museum and Stark Museum of Art.
Make yourself at home in one of the 45 air-conditioned rooms featuring refrigerators and microwaves. Your room comes with a pillowtop bed. Flat-screen televisions with cable programming provide entertainment, while complimentary high-speed (wired) Internet access keeps you connected. Conveniences include desks and coffee/tea makers.
Enjoy the recreation opportunities such as an outdoor pool or make use of other amenities including complimentary wireless Internet access.
A complimentary continental breakfast is included.
Business, Other Amenities
Featured amenities include a business center, complimentary newspapers in the lobby, and laundry facilities.
Hotel Facilities
- Free newspapers in lobby
- Free WiFi
- Business center
- Free breakfast
- Outdoor pool
- Laundry facilities
- Total number of rooms - 45
Room Facilities
- Desk
- Iron/ironing board
- In-room climate control (air conditioning)
- Cable TV service
- Hair dryer
- Free wired high-speed Internet
- Pillowtop mattress
- Flat-panel TV
- Coffee/tea maker
- Free local calls
- Refrigerator
- Microwave
- Air conditioning
Hotel Policy
Know Before You Go
- Children 18 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.


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Carnival Freedom-disappointment: Carnival Freedom Cruise Review by CaroleR
Overall Member Rating
Carnival Freedom-disappointment
Destination: Panama Canal & Central America
Embarkation: Fort Lauderdale (Port Everglades)
Facilities: The buffet the first day was horrendous. Dirty tables, no dinnerware, constantly holding tables to eat upstairs. The good of it was the room steward, some personnel in the dining room and wonderful entertainment and shop personnel (perfume shop). The food started out wonderful in the dining room and then became sporadic. Because there were a lot of families (Thanksgiving), getting a table for breakfast was More difficult. They have built a restaurant based upon a fancy restaurant, but they don't run it as such. Most mornings, we waiting on long lines, but after doing this for several mornings, my husband simply explained to the person in charge that there were tables available seen from the hallway, and we wanted to be seated at one. We were immediately seated. Every day, we were encouraged to go upstairs to the Buffet instead of the dining room.
Room Service: Room service was wonderful.
Dining Room: Most mornings in the dining room, I had to search out the basket of danish, etc. Our dinner companions told us they had run out of bagels and oatmeal mid-cruise.
Facilities: My husband is a cigar smoker and books based upon facilities. The cigar bar was a joke. No TV to watch sports and a constant walk thru for Camp Carnival and passengers who complained of the cigar smoke. If you smoked a cigarette, you could in a few bars. The library had game players in it daily and was not a quiet place to read. The daily newsletter had wrong information most days. As a survivor, I wanted to do the Run for the Cure; a great idea in theory. Asked three people where it was; got 3 answers. Finally gave up and left w/ my shirt and donation given. We always do inside cabin, but this one was much smaller than the other 3 (3 became a familiar # for us) cruises on Carnival. We did meet some wonderful people, but most didn't even crack a smile, unusual for a cruise.
Ports: We stopped in Cozumel, Puerto Limon and Colon. Our first tour was cancelled due to weather (no one's fault) and the second one was a "rip." Will definitely book any excursions on line ahead of time and save money. Also, were told by not to get off of the first port because it was dangerous. They encouraged us to book the excursions. Finally talked to someone honest enough to tell us there was a small flea market right near the boat; we got some cute things very reasonably. Too bad we didn't listen to the cruiseline. They didn't get a rebate on our purchases. This was the tone of the entire cruise.
In essence, next time, Royal Caribbean! Less
Published 12/03/08
Cabin review: 4a7255
Read Cruise Critic's Carnival Freedom Review >>
1st Celeberty Cruise/Will Crui...
Fantastic service and cuisine
SS Mariner
Not my first cruise.
Rough waters, but smooth saili...
3rd Time on the Freedom
Carnival Live on Liberty
Loved the Legend! Best vacati...
Sponsored Links


text:
Originally posted on The Penalty Flag | Last updated 1/4/13
Clutch… ice in the veins… relentless… These are phrases often used to describe NFL quarterbacks who perform best under pressure. Regular season glory is good; just ask Dan Marino. But if you ever want to truly be considered an elite NFL quarterback you have to perform when it counts the most and that's in the postseason. This is a list of guys who got it done in January. Here are the Top 5 NFL Playoff quarterbacks. Honorable Mention: Kurt Warner – St. Louis Rams/Arizona Cardinals: In his 11 year NFL career Warner made the postseason five times and compiled a 9-4 record. That's a .692 winning percentage. Warner led his teams to three Super Bowl appearances; twice with the Rams and once with the Cardinals. Although the Rams had appeared in a Super Bowl when they were in Los Angeles, the city of St.… Read more
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text:
Plum Crumble
Organic plums are a special treat this time of year, and this plum crumble recipe is the perfect combination of sweetness and tartness. Finishing in a brilliant purple color, the crunchy crumbles and tart plum filling make this dish the ideal end to a summer evening BBQ. Pair with fresh vanilla ice cream and top with whipped cream for a truly delectable dessert.
Pro tip: Add some raspberries or blackberries in with the plums for extra summer flavor.
Plum Crumble
- 2 pounds organic plums, cut into ½-inch wedges (about 8 plums)
- ½ cup organic sugar (add 2 additional tablespoons if the fruit is not very sweet)
- 1 Tbsp lemon juice
- 4 Tsp cornstarch
- ½ Tsp coarse salt
- 12 Tbs unsalted organic pasture butter, room temperature
- ½ cup brown sugar
- 2 cups organic all-purpose flour
- ½ Tsp coarse salt
For the Filling
For the Topping
- Preheat the oven to 375 degrees F.
- In a medium bowl, stir together the plum slices, granulated sugar, lemon juice, cornstarch and salt until evenly mixed. Pour the mixture into a 10-inch deep-dish pie tin.
- Beat the butter and brown sugar until combined, then add flour and salt.
- Gather small handfuls of the mixture and squeeze together, then gently break up the clump into large and small pieces and scatter over the filling.
- Bake for 30 minutes, then cover loosely with foil and bake for an additional 20 minutes, until the filling is bubbling.
- Cool for 2 minutes and serve with a scoop of vanilla ice cream.
Recipe adapted from What About Second Breakfast.


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The Washington Times has published an article by Azerbaijani Ambassador to the USA Elin Suleymanov, in which he highlights the 20 January massacre
In .the article headlined "From tragedy to independence", the Ambassador says: "Twenty-five years ago today, while then-President George H.W. Bush was delivering his State of the Union message before a joint session of Congress in Washington, Soviet troops attacked unarmed civilian protesters in the Azerbaijani capital of Baku, killing some 147 people and wounding hundreds of others. The Soviet effort to crush the growing Azerbaijani pro-independence movement culminated in the largest massacre in a generation, it but failed miserably, hastened the end of the USSR, and led directly to our formal declaration of independence two years later.
"The events of 1990 helped crystallize key elements of the Azerbaijani social and political identity. Today two of those elements, namely a strong, unquestionable commitment to its independence and the inclusive nature of its tolerant society, remain at the core of Azerbaijan's strategic importance. Despite a history of imperial domination, the people of Azerbaijan persevered, sacrificed and ultimately won the freedom and independent future they sought. In fact, Azerbaijan was the first nation of the Soviet Empire or Eastern Europe to become free of any foreign military presence on its soil."
Mr Suleymanov says: "Independence has given Azerbaijan the ability to act in the best interest of our own people without outside interference. Azerbaijan's strengths today include energy security, a growing economy, modern infrastructure and a remarkable degree of stability. Independence has allowed us to pursue and achieve our peoples' centuries long dream of a prosperous and successful nation. This is no small accomplishment anywhere, but especially in the complicated geopolitical neighborhood in which we are located."
"Azerbaijan is today a reliable international partner. History has shown that unstable nations with compromised sovereignty pose the greatest threat to peace and security while stable, prosperous societies committed to the fundamentals of international law both promote peace and establish the basis for continued democratic reforms. A stable, prosperous Azerbaijan is good for its people, for the strategically important region in which it finds itself, and for its international partners."
"For a quarter-century, every Jan. 20 citizens of Azerbaijan of all persuasions bring red carnations to the memorial of the tragedy. It is, however, a truly independent, successful and diverse Azerbaijan that is the best tribute to the sacrifice of those who died on the streets of Baku at the hands of a collapsing Soviet regime," says the article.


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A shared flat: well...it's a shared flat!
It's calm , with many gardens and has stores, supermarkets and coffes in a walking distance.
And also is locates near the Metro so you can be in all touristic areas in less than 30 minutes.
To organize a home exchange, you need to be a member


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Members of International Sports Press Association, who are on visit in Baku have visited Ses Media Group oiffice, SIA informs.
The head of Ses Media Group, doctor of philosophy Behruz Guliyev greeted the guests and exspressed his glad see them in the office of the group.
Talking about history of Ses Media group Behhruz Guliyev informed foreign journalists about the history of the group and news bodies into the group; The Ses newspaper(1991), SIA- Ses information Agency ( 2008), Ses internet television (2001) and some news portals. SIA runs in 3 languages ( Azerbaijani, English and Russian).
Then Behruz Guliyev, talked about the care of government for journalists in Azerbaijan. President of Republic of Azerbaijan Ilham Aliyev's order on awarding journalists with apartments on the occasion of 138th anniversary of national press day is a good example of this care. As well, this year on the occasion of the 140th anniversary of the national press will be marked at state level and several journalists will be awarded.
During the meeting, journalists expressed their satisfaction with organization of the first European Games. The games make close people from different European countries. As well, highlighting the hospitability of Azerbaijani people they noted that people are very friendly and warm in Azerbaijan. They smile, help everyone who needs. Martin Schauber( Austria), Alexandr Sotirov(Bulgaria), Karlo Tasler(Croatia) Merili Luuk(Estonia) Sofia Tsikou-Simaioforidou(Greece) Petra Mihalyi(Hungary), Ryan Byrne(Ireland), Steward Said( Malt), Ruslan Medlebek(Kazakhstan) and other foreign journalists expressed their impression about Baku, the capital city of Azerbaijan and the Azerbaijani people.