text:
Broadcast: 15/5/2002
Reporter: Mark Corcoran
CORCORAN: It was the first major sea battle of the 21st century - a savage affair - fought at point blank range - off the coast of northern Sri Lanka last year. This Government gunboat with its 14-man crew is still officially listed as missing in action.But now their fate is no longer a mystery. As these pictures show - they were fatally mauled by Sea Tigers – the maritime arm of the most sophisticated and successful guerrilla organisation in the world today - the LTTE - The Liberation Tigers of Tamil Eelam.For 19 years the Tigers have fought for an independent homeland for Sri Lanka's ethnic Tamil minority. They've proved to be more than a match for Government forces - attempting to blockade rebel-held areas.The Sea Tigers have destroyed a third of Sri Lanka's navy - more than 40 vessels. With blood quite literally running off the decks - the Tigers board the stricken vessel - stripping off weapons and equipment.SINGER: (Translation) "The heroes brought the moonlight to bring smiles to faces - to brighten faces - that were in darkness" CORCORAN: Peace may finally be in the air - but the Liberation Tigers of Tamil Eelam still sing of suffering and sacrifice.SINGER: (Translation)"The black suicide tigers are like the sun that brought light to the suffering of the people. CORCORAN: For the first time in nearly a decade it is possible to cross the front lines into Tiger territory - the self declared Tamil Eelam - homeland for 400 thousand ethnic Tamils. Who today eek out a living amid the ruins of a war that has claimed more than 65 thousand lives.SINGER: (Translation) "The fighters gave their lives as Gods, to carry the lamps to lighten the darkness of the people" (MORNING OATH - TAMIL)"For the freedom of the Tamil nation - we must be faithful"CORCORAN: From the very beginning the Tigers understood the power of propaganda - the need to win - and hold - the hearts and minds.(MORNING OATH - TAMIL)1. "Our great revolutionary movement…" 2."has given us this mission…"3. "for the independence and freedom of our people.."4."We have sacrificed our wealth, body, and thoughts."CORCORAN: And these women form part of the most powerful weapon in an already impressive armoury.…Members of the Niedharsanam unit - a Tamil word meaning reality and truth - they are the "Truth Tigers" (EXPLOSIONS)The Truth Tigers are in the first wave of any attack - recording images of a war fought on a scale few outside Sri Lanka comprehend.13,000 Tigers taking on - and often defeating - Sri Lanka's modern army of 110 thousand - in huge set piece battles.(BUNKER BATTLE)Every unit of one hundred fighters is accompanied by 2 Truth Tigers - armed only with video cameras and suicidal bravery. In any war, truth is the first casualty - and in this conflict the first casualties are often the Truth Tigers - all the camera operators seen here died in these battles.Over the past decade the 120-strong Truth Tiger unit has lost 51 killed and more than a hundred seriously wounded.NADHI: (Translation) "On the battlefield, amid the fighting, we don't think about death - we don't think about escaping - we get enthusiastic - you are so involved in the work – filming - documenting what is happening"CORCORAN: 21-year-old Nadhi is a Truth Tiger veteran. She was indifferent to the Tiger's cause - until an indiscriminate air strike killed her relatives.NADHI: (Translation) "While I was returning from school - they were bombing and my cousins were killed in that incident - I saw this with my own eyes and many people died - and I thought I would die also" CORCORAN: Deciding it better to die fighting she joined the Tigers, aged only 14. Nadhi hasn't seen her mother in years – this has been her family ever since.… The Truth Tigers women's unit which remains strictly segregated from the men except in battle. The role of honour a reminder that life here is about struggle, sacrifice and devotion to the so-called "Elder Brother" Tamil Tiger leader Vellupellai Pirabaharan.NADHI: (Translation) "We meet our "elder brother" very often. When I see our elder brother it is like seeing my mother - he loves us and treats us affectionately - so I don't feel so bad about missing my mother"CORCORAN: Of 19 Truth Tigers who filmed the last campaign 12 were killed in action - so there is a constant need for new recruits.Learning to see the battlefield through the viewfinder of a camera requires a special skill. Live ammunition is used in training - a reminder that mistakes can be fatal.(INSTRUCTOR TO STUDENT Translation:) "They are going to attack so do not stand behind here - because the back blast will get you - you stand to the side to film correctly - OK?(ROCKET FIRES)INSTRUCTOR (Translation): "We are looking for 2 aspects. One - the person has the potential, the courage to undertake this training. The second - to film the fighting beautifully with artistic talent.CORCORAN: It's not only battles that are filmed - every detail of the planning and preparation is also recorded.After the fighting - every order, every move is analysed. It's this attention to detail that makes the Tigers one of the most ruthlessly efficient guerrilla armies in the world.Hidden away in a village are sophisticated video production facilities - crucial to getting the Tigers message out. The chaos, pain and panic of battle is edited out - the focus - on the relentless march to victory - atrocities committed by Government forces - and the heaped bodies of enemy dead.The videos produced here are sent to the half million mainly middle class Tamils now living in Europe, Australia and Canada - who secretly donate about $60 million US dollars a year to the cause - despite the Tigers being declared a terrorist organisation by many western countries.The mastermind of this video campaign for Tamils abroad is the Tiger's second in command – Political Secretary Tamilselvan.TAMIL (Translation): "As far as we are concerned, our people overseas, they must have some relationship with us - they have to know about what is going on here - for example"Q: "But that's an important part, a critical part of your struggle has been to get the money to keep the whole thing going - to buy supplies and weapons - that's well documented?"TAMIL (Translation) "That's not a priority of fundraising – the contributions are not just for strengthening the arms the people abroad are giving money not just for that but also through Tamil organisations - for example - the Rehabilitation Organisation) - because the Sri Lankan Government blockades everything and the peoples lives are disrupted by this" CORCORAN: This is the other crucial element of the Truth Tigers work - recruiting and boosting morale in villages under their control.In a world without communication or entertainment - it's not difficult pulling a crowd for a speech by Tiger leader Pirabaharan.PIRABAHARAN:"No other freedom fighters in the world have achieved the miracles of our movement" CORCORAN: In the secretive world of the Tigers the "Elder Brother" cuts an Orwellian figure - omnipresent - yet invisible. Few ordinary Tamils have ever seen him in the flesh. In theory this is a nationalist movement leavened with Marxism - but in appearance it is more the cult of personality.Elder brother is the Tiger movement: saviour, enforcer, protector from a hostile world. BATTLE VIDEOCORCORAN: The main feature is always a war movie - a dose of reality TV - recording the heroic exploits of the Tigers. Playing tonight - "Unceasing Waves 3".And this is the target audience…The Tigers insist that all recruits are now aged at least 17 - but the United Nations and Amnesty International claim children as young as 12 are still in the ranks. KIDS (Translation) Q: "Tell me what do you think of the videos?KID: We like the fighting. Q: "You like the fighting? KID: Yes... yes. Q: Why do you like the fighting? KID: Because in the fighting between the army and the Tigers - the Tigers win.CORCORAN: With the peace process underway - recruitment has officially stopped - but there are plenty of volunteers here - should the Tigers go back to war.KIDS Q: And when you grow up will you join the Liberation Tigers?KID: Yes Q: When?KID: Not now, When I'm old enough"CORCORAN: Conditioned by a lifetime of propaganda, the next generation of recruits will discover that on the battlefield – there is no glamour – only the certainty of an early death, disfigurement or a life scarred by trauma.TAMIL: (Translation) "Attack! Attack! – Quick! – The tank is going!" CORCORAN: These scenes, filmed by Nadhi, in early 2000, recorded the turning point of the conflict – the Battle for Elephant Pass. When the Tigers over-ran this - the army's biggest, most heavily fortified complex - the Sri Lankan Government finally realized there would be no military solution to this war.BATTLE (Translation) "Brother! Brother! – Don't get in my way! "Come this side! – Move!"CORCORAN: Filming alongside Nadhi was her best friend and camera partner - Yasoo.NADHI: (Translation) We both came together to undertake military training and we stayed together and both of us went to fight together so we were together from the first day. "Yasoo was a person who was anxious to do everything in life. If there was fighting - she was very keen to fight - and she was also very anxious to be there filming the action. CORCORAN: Nadhi and Yasoo filmed what they knew would never be included in the propaganda videos - jammed weapons, raw fear and confusion that clouds even the best-planned battle.NADHI: (Translation) "We both went to the Elephant Pass battle together - when the army was retreating - our boys were attacking the retreating enemy and we had to film them and document the battle to show to the world"CORCORAN: At one point the Sri Lankan soldiers were clearly visible only a couple of hundred metres away - exhausted in their retreat, they didn't even bother to take cover as the Tigers fired.But then Army artillery shells slammed into the Tiger position. Yasoo - standing just metres from Nadhi - was killedFor an instant Nadhi continued filming as her friend's body was carried off - then in shock she dropped the camera.NADHI: (Translation)"When we were filming, she died beside me. With my camera, I was able to document her death... but it affected me somewhat." CORCORAN: Whenever possible Nadhi ventures down this track to visit Yasoo's family - To fulfil a promise made to Yasoo - who had a premonition of her death only days before the battle.Nearly two years on, the shock has passed - but there is very little left to be said as Nadhi and Yasoo's mother Parameswary share a silent grief.NADHI WITH PARAMESWARY: (Translation)Q: "The 2nd anniversary is coming up - are you doing anything for Yasoo?"We will do something"Q: "Are you doing it for both of them?Yes for both of them"CORCORAN: Yasoo's brother Jeyabalan also died fighting for the Tigers. They were the eldest of 9 children - in a family that has nothing to give but its offspring.Like so many in the rebel-held area, they've lived in squalor as refugees since fleeing anti-Tamil riots in the capital Colombo 20 years ago. Nadhi's visits offer some rare comfort.PARAMESWARY: (Translation)"We are grieving, still grieving - we lost our two eldest children under these circumstances - they were fighting - and these children come to us to make us happy - seeing them is like seeing our lost children"CORCORAN: The ceasefire is finally offering some relief from intolerable conditions in Tiger territory.Minefields are being cleared and civilians are now permitted to cross the frontlines – closely monitored by the Sri Lankan Army.Despite hardships imposed by the military and economic blockade there's still fierce support for the Tiger cause – which the Army's Northern Commander attributes to the propaganda work of the "Truth Tigers".MAJ-GEN. SENAVIRATNQ: Is it a powerful weapon? MAJ-GEN. SENAVIRATNA:. It is definitely a powerful weapon - you have to accept it"CORCORAN: Yet incredibly, in this age of information warfare - it is a weapon the army has refused to use.Preferring to rely on old- fashioned censorship, communiqués - and keeping cameras off the battlefield.MAJ-GEN SENAVIRATNA:"As far as the security forces are concerned, whenever we have successes of this nature we do not sort of video it - then propagate that. Because seeing lots of dead bodies is not a good sign so therefore we don't do it we had lots of dead bodies of LTTE cadres - but we never use them for propaganda because it is detested by the population".CORCORAN: But there are some operations even the Truth Tigers don't film – blatant acts of terrorism that are excluded from the propaganda videos.In this 1996 attack on the Central Bank in Colombo by a Black Tiger suicide squad, 91 people died, 1,400 were wounded - nearly all of them civilians.The Tigers have transformed suicide bombing into a science - Black Tigers have carried out 200 such bombings and political assassinations.(TAMILSELVAN)Q. "By any definition you are also a terrorist organisation - you send suicide bombers against civilian targets - no matter what the Government has done to you - you send people against civilian targets in major cities"TAMILSELVAN: "when they were attacking and destroying hundreds of people - we have to respond using certain strategies (41) and during that time - it is true that some of their civilians were affected - that we accept".CORCORAN: Taking responsibility for acts of terrorism isn't the only stumbling block to peace in Sri Lanka.If there is to be reconciliation - the issue of war crimes also needs to be confronted. This is another - albeit grisly task of the Truth Tigers - documenting the dead. In this war without rules or compassion - few prisoners are taken.Nadhi's unit recorded these pictures after the Tigers overran the Kilinochchi army camp in '98 - killing more than a thousand troops - there were no prisoners.Earlier, when another military base at Mullativu was captured there were 1,500 dead - again no prisoners.The Tigers claim - somewhat implausibly that the army always fights to the last man. But the military has another explanation.MAJ-GEN SENEVIRATNE:"I think the LTTE kills most security forces personnel - who even surrenders - as we've seen in the Mullativu operation - and maybe because of administrative problems that they may be adopting this system.Q: Administrative problems?SENEVIRATNE: Of the LTTE.Q: So, sorry, just explain that?SENEVIRATNE: Yes, when you take a large number of POW's you have to administer them - and you have to have places to keep them - and you have to register them and show them to the ICRC - so that's the reason".CORCORAN: But many Tigers regard this as due retribution. Government massacres of Tamil civilians have been well documented - and captured Tigers are routinely tortured.All Tigers carry a cyanide capsule - Nadhi's placed around her neck by the "Elder Brother" himself - with orders to commit suicide when facing capture.NADHI:"If it looks like we are going to be captured by the enemy on the battlefield - we then bite this capsule - and it will kill us - so as to protect our military secrets from the enemy - The secrets are more important than our lives – Our elder brother – the leader tied this to me – I've worn it ever since.CORCORAN: The Tigers have now launched another propaganda offensive – this time aimed at the world's media.Recently, the Truth Tigers organized a conference for 300 members of the international press.Displaying the trademark obsession with security - they methodically filmed everyone in attendance. Since the September 11 attacks in the United States – the term terrorist has carried a powerful new connotation. It was the Americans declaration of war on global terrorism that finally pushed the Tigers towards the peace negotiation table. For the first time in nearly a decade "Elder Brother" Pirabaharan emerged from the shadows – wearing a civilian suit of reconciliation instead of his usual Tiger stripe fatigues. CORCORAN: The Tigers have now launched another propaganda offensive – this time aimed at the world's media.Recently, the Truth Tigers organized a conference for 300 members of the international press. Displaying the trademark obsession with security - they methodically filmed everyone in attendance.Since the September 11 attacks in the United States – the term terrorist has carried a powerful new connotation. It was the Americans declaration of war on global terrorism that finally pushed the Tigers towards the peace negotiation table. For the first time in nearly a decade "Elder Brother" Pirabaharan emerged from the shadows – wearing a civilian suit of reconciliation instead of his usual Tiger stripe fatigues. But as a gesture it failed. This was a press conference of platitudes – a media event long on symbolism but short on substance.PIRABAHARAN: (Translation)"When a permanent solution is reached – we will not only bring an end to all forms of violence – but seriously consider removing the cyanide pills from all our fighters" CORCORAN: If the war resumes - Nadhi fully expects to die - to one day lie here alongside her friend Yasoo.This is just one of eight martyrs cemeteries in Tiger territory. Resting place for a total of 17 thousand fighters - mostly teenagers - who died a so-called victorious death.NADHI: (Translation)"When we were together - if there was a battle she used to say to me - if I die a victorious death - will you come and visit my grave? She used to say this - so I used to reply "why should I come to your grave if you die a victorious death? She would say that I was her life friend and I should come" CORCORAN: Most Tigers are Hindu, and custom dictates they should be cremated - but Elder Brother Pirabaharan insists on burial - as a potent symbol of sacrifice.NADHI:"Whenever we have the time we come. Anytime. Sometimes even at night. We'll come here place flowers and go"CORCORAN: And it is here that truth finally is revealed. For a Tiger's real name is only announced upon death - painted on a headstone."A Tamil homeland or a victorious death" was the call that sent many to an early grave here - but for peace to have any chance of succeeding that all-or-nothing demand will have to be moderated - and compromise has never been a trait of the Tigers.But already there are small signs of hope. Now for the first time, grass grows on the empty graves awaiting the next batch of martyrs.
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© 2002 Australian Broadcasting Corporation
Email: firstname.lastname@example.com


text: IRVINE, Calif., June 14, 2013 /PRNewswire/ -- Kawasaki Motors Corp., U.S.A. (KMC) announced a unique tie-in with "The Lone Ranger," a Disney/Jerry Bruckheimer Films production set for national release on July 3. The powersports products marketer will use this new reimagining of the popular story to help carry product messages for its off-road motorcycles, four-wheel vehicles and street motorcycles. Concentrating on a "Live the Legend" campaign line, Kawasaki's effort is heavily oriented to video creations depicting a transition from the Gore Verbinski-directed film to scenes of modern day adventurers with the company's Teryx4 ™ Side x Side, KX ™ motocross, and Ninja ® sportbike products. The action-oriented "Live the Legend" video appears on KawasakiTV's YouTube channel, at: bit.ly/LiveTheLegend. Similar materials featuring the film's stars, Johnny Depp and Armie Hammer, are also slated to appear in more than 1,000 Kawasaki dealerships around the country. Dealers will also be a core component of Kawasaki's "Live the Legend Adventure" sweepstakes, which will invite consumers to text entries for a chance to win various products inspired by "The Lone Ranger." The sweepstakes will also include film promotional partner, the Utah Office of Tourism. "This is an opportunity for Kawasaki to generate a great deal of excitement around product categories that are a perfect fit for the film's characterizations," said Chris Brull, Kawasaki's marketing director. "We appeal to the same type of independent, adventurous, rebellious individuals depicted by the film's stars," said Brull. "The Lone Ranger"-inspired Kawasaki vehicles will be featured as prizes in other film tie-in promotions, including five limited edition Kawasaki Teryx4 750 4x4 LE Side x Sides to be given away by SUBWAY ® Restaurants in their Partner Up & Win promotion, and a Teryx4 and four Shoei off-road helmets as the grand prize for an ABC-TV "Good Morning America" sweepstakes. In addition to media and in-store involvement supporting the film's release, Kawasaki is also providing a custom-designed Lone Ranger Ninja ZX-6R sportbike that will be on display at the movie's world premiere. That event is a benefit for the American Indian College Fund, and the unique motorcycle will be signed by the film's stars and other celebrities in attendance. It will then be auctioned at a later date with all proceeds going to support the fund. Kawasaki Motors Corp., U.S.A. markets and distributes Kawasaki motorcycles, ATVs, Side x Sides, and personal watercraft through a network of more than 1,200 independent retailers, with an additional 7,400 retailers specializing in Kawasaki power products and general purpose engines. KMC and its affiliates employ nearly 3,100 people in the United States, with 300 of them located at KMC's Irvine, California headquarters. Kawasaki's tagline, "Let the good times roll. ™", is recognized worldwide. For five decades, the Kawasaki brand has become synonymous with powerful, stylish and category-leading vehicles. Information about Kawasaki's complete line of powersports products and Kawasaki affiliates can be found on the Internet at www.kawasaki.com .

subset: commoncrawl


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Sorry i'm new to comics... basically i have read only 1 = Superman Doomsday.
Me personally i like that one because the fight scenes was consistent, none of the b.s crap that makes Superman so powerful in other comics.
The fight between Superman and Doomsday was realistic and very enjoyable to read and watch.
I have also watched the original Superman movie played by Reeve and written by Mario Puzo.
If Superman's strength is determined by its bone density and the power of the sun, and the origins of Superman was already established, what this Superman Prime? I was reading through the Wiki it said something about time travel.
What is the need for the DC guys to create a character like Prime?
Isn't normal Superman popular enough?


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|Half Baths
|430 sq. ft.
|Property type
|Minimum Stay
|5 nights
Welcome to our lovely apartment, a two-room newly renovated and located close to the Eiffel Tower and Champ de Mars. You can enjoy the view of the Eiffel Tower while taking a meal prepared in the new kitchen. To rest after your long days of walking, you will enjoy the queen bed room located in a quiet courtyard. You have indeed many visits to walk from this apartment: romantic walks along the Seine, museums around or do your shopping in the Rue Cler with its permanent market. In the evening you can enjoy the wide choice of restaurants (starred or not) nearby.
Eiffel Tower view, quite bedroom, sunny appartment.
We loved staying in Nathalie's Eiffel view apartment in the 7th. Three blocks to the tower, three blocks to the Metro, three blocks to the River, and three blocks to the Rue Cler and other local markets -- who could ask for more? But there is more - a nice, modern, clean and well kept apartment with all the necessities; a lovely hostess with great accessibility and suggestions; and a dead on view of that tower which puts on a sparkling shown on the hour every night !
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This was our 5th time visiting Paris. This was by far the nicest apartment we have ever stayed in. The location is a 5 min walk from the Eiffel Tower, Rue Cler and RER C metro and just seconds from great cafe's and a bakery. The apartment itself was beautiful. Tastefully remodeled and spotless. The view from the living room was absolutely incredible. The English speaking owner met us at the apartment and was very helpful.
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Apartment very central.
Eiffel Tower, Champ de Mars Park, Quai de Seine,
Paris Bus Tour, Bateaux Mouches,
Branly Museum, Orsay Museum, Invalides, Napoleon's Tomb, the Army Museum, Rodin Museum, the Trocadero, the Museum of Man, the Navy Museum, Palais de Chaillot, the Grand Palais and Petit Palais
Standing Rue Cler market
Metro / RER: Pont de l'Alma and Ecole Militaire
Many starred restaurants.
|Rate Period
|Weekend Night
My Standard Rate
5 night minimum stay
Final payment on arrival
Check in : after 3 pm
Check out : before 11 am


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The Natchez SolutionBrandon, Mississippi United States of America
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May 26th, 2017
Where to Sample ON 5/27 & 5/29
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Burton Ambush Snowboard Boots 2013 - $149.95
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Adsorption isotherm and heat of sorption of fresh- and osmo-oven dried plantain slices
- First Online:
- Received:
- Revised:
DOI: 10.1007/s00217-003-0738-8
- Cite this article as:
- Falade, K.O., Adetunji, A.I. & Aworh, O.C. Eur Food Res Technol (2003) 217: 230. doi:10.1007/s00217-003-0738-8
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Firm ripe plantain was transversely cut into 10-mm slices and osmotically pretreated in 52 °, 60 ° and 68 °B sucrose solutions, for 12 h at 25 °C. Fresh- and osmosed plantain slices were air-dried in a cross flow forced draught oven at 60 °C for 72 h. Adsorption isotherms of the products were determined at 20 °C and 40 °C, using a gravimetric-static method. Adsorption data were fitted into eight isotherm models. Isosteric heat of sorption was calculated using the Clausius-Clapeyron equation. Adsorption isotherms of fresh- and osmo-oven dried plantain slices followed type I (J-shaped) isotherms, characteristic of high sugar products. Inversion of 20 C and 40 C isotherms occurred between aw 0.65 and 0.70. Among the models tested, the Guggenhein Anderson deBoer (GAB) gave the best fit. Isosteric heat of sorption increased with decreasing moisture contents. Negative isosteric heat of sorption occurred at high moisture content. Moreover, isosteric heat of sorption increased with increase in sucrose solution concentration during the osmotic dehydration prior to oven drying.


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More pressure on education budget
Tasmania's Education Union predicts the State Government will be forced to find an extra $80 million a year under school funding changes recommended in the Gonski report.
The Tasmanian Government is yet to detail its response to the report which recommends state and federal governments inject an extra $5 billion a year into the education system to address disadvantage.
The union's state president, Terry Polglase, estimates it will cost the cash-strapped State Government an extra $80 million a year.
Mr Polglase says the state is cutting $190 million out of education over four years.
"Where are they going to find this money? I think it's incumbent really on the Federal Government to consider paying the entire $5 billion," he said.
"It is there to address disadvantage, that's what our taxpayers' money is there for, and at the moment it actually does so.
"It pays national partnership money and that only goes to schools where there is need. Now here is $5 billion worth of need."
The Education Minister, Nick McKim, has not spoken since last Thursday's meeting of state ministers.
"I've had a conversation with Nick McKim and he thinks I'm very close to the figure," Mr Polglase said.
The Minister has previously welcomed any funding model that puts more money into state schools and helps disadvantaged students.
The Opposition's Vanessa Goodwin says the State Government may never be able to implement the Gonski' report's recommendations.
"Obviously the State Government has blown the budget over many years, a billion dollars over the last six years and they have to find $190 million in savings in the Education Department," she said.
Topics: schools, budget, federal---state-issues, tas, hobart-7000, launceston-7250
First posted


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USCAA Staff
| The Bolles School is a private college preparatory school with an international reputation for excellence. Located in Jacksonville, Florida, the school enrolls more than 1,700 boarding and day students in pre-kindergarten through grade 12 on four campuses. Bolles focuses on a complete education that emphasizes excellence in academics, the arts, athletics and other activities like clubs and community service. Students can pursue their interests and learn to balance a rich variety of activities.
The internationally recognized athletic program at Bolles provides students in middle and upper school the opportunity to learn, grow, and develop into well-rounded individuals. Participation and camaraderie are as important as competition and success in the overall educational aspect of the athletic program. While Bolles teams and athletes are often recognized for outstanding achievements and results on the field, the important lessons of leadership, self-discipline, and teamwork are the lasting rewards possible through athletic involvement at the School.
With 42 teams at the middle, junior varsity, and varsity levels, Bolles offers a full complement of athletic opportunities. The student body regularly approaches or exceeds 90% participation in one or more sports.
Purpose: To organize, direct, and sustain a competitive men's interscholastic and club rowing program. This position will work closely with the Head Coach for Women's Rowing to lead a team of 4-6 Assistant Coaches and approximately 140 rowers in grades 6 through 12. The Head Coach serves as a motivational figure who inspires high morale, builds team cohesiveness, communicates effectively, and provides positive reinforcement to our student athletes. This is a 12-month, full-time position.
Essential Functions: • Responsible for all facets of the Men's Rowing Program • Supervise athletes during practices and regattas, including general warm-up, race preparation and race analysis • Establish an environment that fosters positive team attitudes, encourages self-discipline, sportsmanship, leadership, responsibility, and an appreciation for the importance of academics • Prepare and communicate season objectives and outline for training plan(s) • Uses appropriate and varied coaching techniques; prepares and conducts regular assessments of students' athletic and behavioral attitudes and achievements • Assigns workouts and gives feedback in a timely manner • Teaches rules of rowing and boathouse protocol • Maintains order in and around boathouse and on the water, adhering to safety protocols • Inspire confidence in the athletes, coaches, and parents • Advise and counsel rowers who wish to pursue junior development opportunities and/or collegiate competition, including writing letters of recommendation, if requested • Oversee participation in local, state, and national meets and regattas • Lead the fall and spring crew programs, scheduling, and supervising daily practices and off-season training (winter/summer conditioning programs and camps) • In partnership with the Women's Head Coach, develop and sustain a youth rowing program, separate from the scholastic rowing program • Review, enhance and enforce all safety procedures • Ensure that Assistant Coaches and participants are properly trained and conditioned • Co-manage the physical operation, maintenance and inventory of the waterfront facilities--- boathouse, docks and equipment at our state-of-the-art boathouse and crew complex • In partnership with the Women's Head Coach, coordinates race transportation, season race schedule, and travel arrangements • Coordinate with Finance Office for proper billing and collection of club fees for the program and handle business side of the club • Design and implement department plans and budgets • Manage special events related to Crew; coordinate the seasonal athletic awards assembly • In partnership with Marketing, provide input in preparation and dissemination of publicity to promote events • Work with Administration, parent leadership, and committees to manage and grow the program • Ensure compliance with all applicable Florida Scholastic Rowing Association and USRowing rules and regulations • Ensure compliance with all applicable FHSAA and Bolles Athletic Department policies and procedures • Responsible for the selection, supervision, and evaluation of Assistant Coaches supporting the Men's program


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Have you been considering it for a long time, but always postpone finishing your foundation project?
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Renovation projects with the best return on investment
In many cases, investing in some specific home improvement projects can be quite profitable down the road. Here is a list of 8 projects that yield superior return on investment :
Renovate your kitchen
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New toilets and tubs
A midrange bathroom upgrade will always remain a great investment in the value of the house.
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Nanoplasmonic triple-wavelength demultiplexers in two-dimensional metallic waveguides
- First Online:
- Received:
- Revised:
DOI: 10.1007/s00340-011-4525-0
- Cite this article as:
- Lu, H., Liu, X.M., Wang, L.R. et al. Appl. Phys. B (2011) 103: 877. doi:10.1007/s00340-011-4525-0
A novel kind of plasmonic wavelength demultiplexers (WDMs) based on two-dimensional metal–insulator–metal waveguides with side coupled nanocavities (SCNCs) is proposed and numerically investigated. The WDMs contain three waveguide output channels, each of which functions as a dual-stopband plasmonic filter. The demultiplexing wavelengths can be tuned by controlling the lengths and widths of SCNCs. The finite-difference time-domain results can be accurately analyzed by the resonant theory of nanocavity. Our structures have important potential applications for design of WDM systems in highly integrated optical circuits.
Copyright information
© Springer-Verlag 2011


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You Sing: Elvis Presley... Sun Years (Karaoke CDG)
You Sing: Elvis Presley... Sun Years (Karaoke CDG) pop/Rock. Karaoke CDG. Published by Pocket Songs (PS.PSCDG1057).
Elvis Presley began his career at Memphis' Sun Studios, a little recording company on Union Avenue that is considered by many to be the birthplace of rock and roll. Many legends got their start there, but none would become bigger stars than Elvis Presley. This karaoke collection features some of Elvis' great early songs that were originally recorded at the legendary studio, including That's Alright Mama, The King's very first single.
Each Pocket Songs PSCDG album contains a complete performance with a professional vocalist as well as the karaoke tracks of each song, for the at-home performer. CDG discs can be played on a regular CD player but require a CDG player to see the graphics.


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Iconic Hollywood glamour mixes with modern sophistication and intuitive service with the transformation of the Bel Age hotel into The London West Hollywood. This famed location on the legendary Sunset Strip has undergone a complete re-articulation by the bold team of international tastemakers responsible for a stylish sister, The London NYC. Distinctively imagined for LA, interiors are by the renowned David Collins Studio, dining by chef Gordon Ramsay, and bath environments by posh purveyor Waterworks.
Timeless style, attuned service, open floor plan suites for business and pleasure, smashing food and drink, a rooftop pool with panoramic views and all the personal amenities one could desire, The London's sunny sophisticated style offers an eclectic and personal lifestyle experience. The London West Hollywood will be both your 'all-access' threshold to the LA social and entertainment scene as well as your soothing haven from the pulse.


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Good afternoon, I've got a query about limited by guarantee companies. Everything I've seen suggests that a limited by guarantee company is the correct (or at least most suitable) company structure for my new venture, which is a table tennis school. Sports societies and academies both seem to fall under the limited by guarantee category. That being said, I'm not trying to start a charity. This business will be my livelihood, and I'd like to make some money through running it. But so many charities are limited by guarantee companies that it has got me wondering: am I allowed to run a limited by guarantee company for profit?
Browse other questions tagged Ask a new questionor


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Evaluation of Cross-Language Information Retrieval Systems
€ 128,49
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August 2002
BeschreibungThe second evaluation campaign of the Cross Language Evaluation Forum (CLEF) for European languages was held from January to September 2001. This campaign proved a great success, and showed an increase in participation of around 70% com pared with CLEF 2000. It culminated in a two day workshop in Darmstadt, Germany, 3-4 September, in conjunction with the 5th European Conference on Digital Libraries (ECDL 2001). On the first day of the workshop, the results of the CLEF 2001 evalua tion campaign were reported and discussed in paper and poster sessions. The second day focused on the current needs of cross language systems and how evaluation cam paigns in the future can best be designed to stimulate progress. The workshop was attended by nearly 50 researchers and system developers from both academia and in dustry. It provided an important opportunity for researchers working in the same area to get together and exchange ideas and experiences. Copies of all the presentations are available on the CLEF web site at http://www. clef campaign. org. This volume con tains thoroughly revised and expanded versions of the papers presented at the work shop and provides an exhaustive record of the CLEF 2001 campaign. CLEF 2001 was conducted as an activity of the DELOS Network of Excellence for Digital Libraries, funded by the EC Information Society Technologies program to further research in digital library technologies. The activity was organized in collabo ration with the US National Institute of Standards and Technology (NIST).
InhaltsverzeichnisSystem Evaluation Experiments at CLEF 2001.- CLEF 2001 - Overview of Results.- Mainly Cross-Language.- Report on CLEF-2001 Experiments: Effective Combined Query-Translation Approach.- Multilingual Information Retrieval Using English and Chinese Queries.- Exeter at CLEF 2001: Experiments with Machine Translation for Bilingual Retrieval.- TNO at CLEF-2001: Comparing Translation Resources.- ITC-irst at CLEF 2001: Monolingual and Bilingual Tracks.- Experiments with the Eurospider Retrieval System for CLEF 2001.- Using Co-occurrence, Augmented Restrictions, and C-E WordNet for Chinese-English Cross-Language Information Retrieval at CLEF 2001.- Utaclir @ CLEF 2001 - Effects of Compound Splitting and N-Gram Techniques.- Using Statistical Translation Models for Bilingual IR.- Cross-Lingual Pseudo-Relevance Feedback Using a Comparable Corpus.- Investigation on Disambiguation in CLIR: Aligned Corpus and Bi-directional Translation-Based Strategies.- Vector-Based Semantic Analysis Using Random Indexing for Cross-Lingual Query Expansion.- Query Expansion Techniques for the CLEF Bilingual Track.- Intelligent Information Access Systems (SINAI) at CLEF 2001: Calculating Translation Probabilities with SemCor.- JHU/APL Experiments at CLEF: Translation Resources and Score Normalization.- Dictionary-Based Thai CLIR: An Experimental Survey of Thai CLIR.- English-Dutch CLIR Using Query Translation Techniques.- Thomson Legal and Regulatory at CLEF 2001: Monolingual and Bilingual Experiments.- Working with Russian Queries for the GIRT, Bilingual, and Multilingual CLEF Tasks.- IR-n: A Passage Retrieval System at CLEF-2001.- Monolingual Experiments.- Spanish Monolingual Track: The Impact of Stemming on Retrieval.- Shallow Morphological Analysis in Monolingual Information Retrieval for Dutch, German, and Italian.- Stemming Evaluated in 6 Languages by Hummingbird SearchServer(TM) at CLEF 2001.- Minimalistic Test Runs of the Eidetica Indexer.- Across the Bridge: CLEF 2001 - Non-english Monolingual Retrieval. The French Task.- Mpro-IR in CLEF 2001.- Some Terms Are More Interchangeable than Others.- Interactive Track.- The CLEF 2001 Interactive Track.- Noun Phrase Translations for Cross-Language Document Selection.- iCLEF at Sheffield.- iCLEF 2001 at Maryland: Comparing Term-for-Term Gloss and MT.- Evaluation Issues and Results.- The Philosophy of Information Retrieval Evaluation.- CLIR System Evaluation at the Second NTCIR Workshop.- Multilingual Topic Generation within the CLEF 2001 Experiments.- CLEF Methodology and Metrics.
Untertitel: Second Workshop of the Cross-Language Evaluation Forum, CLEF 2001, Darmstadt, Germany, September 3-4, 2001. Revised Papers. 2002. Auflage. Book. Sprache: Englisch.
Verlag: Springer
Erscheinungsdatum: August 2002
Seitenanzahl: 616 Seiten
Format: kartoniert


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Creative Nonfiction Prize
Linda Rosenbaum on finding moments of joy in sadness
There are five names on the shortlist for this year's CBC Creative Nonfiction Prize. Before we announce the winner, we want to introduce you to the finalists and their stories.
Linda Rosenbaum talks about loving roses and hamburgers, the importance of educating people about Fetal Alcohol Syndrome, and what it's like to write about family.
Tell us about yourself.
I'm a mother, wife and friend—roles that clearly mean the world to me since they're the first words that came to mind. I write, edit, cook, garden, dance and cycle. I love roses and a really great hamburger, particularly if accompanied by equally good fries. I cry easily, whether happy or sad, and wish I could get more sleep. I live on Toronto Island.
What do you usually write?
I'm a curious person, so other than poetry and fiction which I do not write, just about anything is game. If something piques my interest, I'll write about it. That covers a lot of territory. Professionally, I often write about health and medical issues.
Entries on my blog in the past year were, what some might say, all over the place. In one entry I revealed a secret to making a great chicken soup (parsnips). I gave a list of collective nouns for animals in another, (murder of crows, lamentation of swans, parliament of owls... ), and in another, challenged readers to write six word memoirs ("Not quite what I was planning." "According to Facebook, we broke up.").
I also write about Fetal Alcohol Syndrome (FAS), a condition my son has. Too many people are still not aware of the irreversible harm from drinking alcohol when pregnant. While Asperger's Syndrome has made its way into the public's radar, FAS has not, and it's a shame, because FAS is preventable.
What made you write this story?
This piece is actually the epilogue of a memoir I have been writing, some of which has to do with the challenges of raising our son. I wanted to end the book with a positive feeling, which I hadn't yet done. So when I read about this competition, it encouraged me to write "Wolf Howling at Moon."
I hoped to show how those of us who live with sadness, particularly in regards to our children, must relish the moments of joy that come our way. They're not always easy to find, but if we can shift our dreams and expectations, we may make it easier for ourselves.
Did you tell the people implicated in your story that you were writing it?
Yes, my family knows knows about this piece. My husband knew when he married me that I was a writer, and one day would likely turn him into subject matter, so he takes it all rather well. My daughter is a little less comfortable, but now accepts that I have been writing a memoir and can't exactly leave her out. That said, I'm very careful what I say about her.
Writing about my son, in this piece and the memoir, is a little more complicated because I talk about his disabilities and the struggles we have had raising him. If I didn't feel there was a greater good from telling our story, I would have had to stop. But I have nothing but love and compassion for Michael. I hope this overarching message comes through loud and clear when I write about him.
You mention three past events in your story: Michael's arrival home, his birthday party, and his diagnosis. What was important about those particular events?
These three events reflect a seismic shift in what it was like to be Michael's parent. When we brought him home from the hospital, we were like all new parents—hopeful, optimistic and full of dreams. I thought a good way to convey these feelings was to describe the scene of our homecoming at the ferry docks.
Five years later, I describe the birthday party we had for Michael with the many boys in our community who were born the same year as Michael. While the party was great fun, and all was well on the surface, clouds were setting in. My husband and I were seeing how different Michael was from the other boys he was playing with. Sure enough. With time, we watched these boys grow up and move on in their lives in ways Michael never has.
One year later, we had the diagnosis. Fetal Alcohol Syndrome. His birthmother drank when pregnant with him. The diagnosis confirmed what we already knew—Michael was damaged. No easy fixes. The road ahead would continue to be rough, and there was no turning back.
You describe three of Michael's carvings. What do you think is so important about these images, for him, and for you?
Michael finds comfort and safety in nature and with animals, less so with people. So it's not surprising that he chose to carve a wolf, a bass and a bear. Since he was young, Michael has dreamed of living on his own, self-sufficient, in the wilderness.
Michael has heard wolves howling while on camping trips with us, and that very well might be his reference. When I asked Michael why he chose to carve a wolf howling at the moon, he didn't have an answer, he said, "I like wolves."
It's very possible that I read more into "Wolf Howling at Moon" than Michael does. As a mother and writer, I am always trying to understand how Michael thinks and feels. A wolf is a strong metaphor in many cultures. Though they are actually social creatures who live in packs, there is a mythology about wolves as solo creatures. These lone wolves, like Michael, prefer to live alone and independent rather than in a group.
How does it feel to be shortlisted for this prize?
Nothing less than thrilling. It feels great to have my work recognized and share my story with a broader audience than I could otherwise,
And how can I not feel love for the CBC and Canada Writes for honouring and celebrating the written word.


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If you are determined to get placed in TCS, then you should compulsorily know these tricks to get placed.
Trick #1: TCS Written Test Questions are repeated
Based on the past campus recruitment patterns, 80% of the question types are repeated. Practice more to get placed. Practice each and every question type asked in the previous year papers and get complete clarity. PlacementSeason is the best location for practicing TCS Patterns.Our mock tests are similar to the original placement test and many questions are from the previous recruitment drives. Practice here…
Trick #2: Focus on High Weightage Questions
Last year, TCS introduced few questions with more marks. You can identify them with * mark. Allocate more time to these questions to clear the cutoff.
Trick #3: Practice more Puzzle Based Questions
In 80 minutes, you are expected to attempt 30 questions. Time per question may seems to be comfortable. You should understand that many questions are tricky and require more time to solve. More practice can only help in this case.Practice TCS questions here…
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Without enough English writing practice, you cannot clear the email writing section because in just 10 minutes, you have to complete email with the given key words. Use PlacementSeason's Writing Skills Assessment Tool again and again to improve your English writing skills. Know more..
Practice and more practice is the winning formula that we deliver to the deserving students to get placed in their dream company. What are you waiting for?


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BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
Bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. Based on our analysis of a diverse collection of 16 S rRNA gene sequences, we designed a broad-coverage quantitative real-time PCR (qPCR) assay—BactQuant—for quantifying 16 S rRNA gene copy number and estimating bacterial load. We further utilized
The aligned core set of 4,938 16 S rRNA gene sequences in the Greengenes database were analyzed for assay design. Cloned plasmid standards were generated and quantified using a qPCR-based approach. Coverage analysis was performed computationally using >670,000 sequences and further evaluated following the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines.
A bacterial TaqMan® qPCR assay targeting a 466 bp region in V3-V4 was designed. Coverage analysis showed that 91% of the phyla, 96% of the genera, and >80% of the 89,537 species analyzed contained at least one perfect sequence match to the BactQuant assay. Of the 106 bacterial species evaluated, amplification efficiencies ranged from 81 to 120%, with
The BactQuant assay offers significantly broader coverage than a previously reported universal bacterial quantification assay BactQuant in vitro performance was better than the
Scientists today are studying bacterial communities from diverse habitats, hosts, and health conditions based on the 16 S rRNA gene
Among culture-independent approaches, quantitative real-time PCR (qPCR) is currently best suited for measuring bacterial load, because of its intrinsic quantitative capability, ease of use, and flexibility in assay design
In the current paper, we present our design and validation of a broad-coverage quantitative real-time PCR assay—BactQuant—for quantifying 16 S rRNA gene copy number and estimating bacterial load. To accomplish this, we have employed a novel nucleotide distribution-based approach to effectively summarize a large 16 S rRNA gene sequence dataset for qPCR assay design. We further addressed a general limitation of the qPCR platform—the normalization of in-run quantitative standards using fluorimetric or spectrometric methods—by developing an alternative qPCR-based method for quantifying plasmid standards. Lastly, we have complemented standard qPCR assay validation following MIQE guideline
Design of 16 S rRNA gene quantitative real-time PCR assay
Pre-aligned 16 S rRNA gene sequences (n = 4,938) were downloaded from the core set of the Greengenes database
Assay Design
The nucleotide distribution was examined to identify a conserved 500 bp region for assay design. In designing the assays, we applied the following rules: 1) primer sequences cannot have more than three degenerate bases and 2) the probe sequence cannot have any degenerate bases. The primer Tm was calculated using salt adjusted calculation from the online tool OligoCalc
Computational analysis of assay specificity and coverage
A. Specificity analysis. Specificity check was performed in GenBank using megablast against human, mouse, and fungal sequences from the nucleotide collection (nr/nt)
B. Collection and identification of bacterial 16 S rRNA gene sequence eligible for
C. Overview of sequence matching analysis for determining assay coverage. All sequence matching for the
D. Numerical coverage analysis. At each analysis level, unique operational taxonomic unit (OTU), i.e., each unique taxonomic group ranging from unique phyla to unique species, containing at least one sequence that is a sequence match (i.e., "match") for all three components of the assay of interest were identified using the following requirement: [Forward Primer Perfect Match](union)[Reverse Primer Perfect Match](union)[Probe Perfect Match]. The
E. Taxonomic coverage analysis. The
Additional file 1
Figure S1. Figure S1 containing the
Click here for file
F. Assay comparison using results from the
Additional file 2
Figure S2A-E. Standard curve amplification plots using mixed templates.
Click here for file
Figure 1
Results from in silico coverage analysis of the BactQuant assay using the stringent criterion against 1,849 genera and 34 phyla showing broad coverage
Results from
Quantification and normalization of cloned plasmid standards
To obtain accurately quantified plasmid standards for validation the BactQuant assay, a 109 copies/μl plasmid stock was quantified using a qPCR assay targeting portion of the vector using the second derivative maximum analysis algorithm on the LightCyler platform. The resultant crossing point value (i.e., Cp-value) is used in plasmid normalization. The details are as follows:
Generation of normalized 16 S rRNA gene plasmid standards
Amplification of the full 16 S rRNA gene was performed using
Pan-bacterial qPCR assay optimization and initial specificity check
Assay optimization
Using the normalized plasmid standards, different primer and probe titrations were tested on the on the 7900HT Real Time PCR System (Applied Biosystems) and evaluated based on reaction efficiency and assay dynamic range for 10 μl and 5 μl reaction volumes. For 10 μl and 5 μl reactions, the optimized conditions included 1 μl of template into 9 μl and 4 μl of reaction mix, respectively, with the final reaction containing 1.8 μM of each forward and reverse primer, 225 nM the TaqMan® probe, 1X Platinum® Quantitative PCR SuperMix-UDG w⁄;ROX (Invitrogen Corp.) and molecular-grade water. Irrespective of reaction volume, each experiment included an in-run standard curve (102–108 in 10-fold serial dilutions) and no-template controls performed in triplicate. Amplification and real-time fluorescence detections were performed on the 7900HT Real Time PCR System (Applied Biosystems) using the following PCR conditions: 3 min at 50°C for UNG treatment, 10 min at 95°C for
Initial specificity check against human and fungal genomic DNA
Using the optimized assay condition, the newly designed assay was tested against 1 ng, 100 pg, and 10 pg of human genomic DNA (Promega, Madison, WI, USA),
Laboratory analysis of assay performance using diverse bacterial genomic DNA
To assess our assay performance against diverse bacteria, we tested our assay against a diverse collection of bacterial genomic DNA to determine the assay efficiency and correlation coefficients. The details are as follows:
Bacterial strains
Extraction of bacterial genomic DNA
Extraction using the enriched broth was performed using ZR Fungal/Bacterial DNA MiniPrepTM (Zymo Research, Irvine, CA, USA) following the manufacturer's instruction. Elution of the purified genomic DNA was performed using 100 μl of 1X TE buffer.
Other sources of bacterial genomic DNA
Genomic DNA from
Experimental design
For sensitivity and efficiency analysis, bacterial genomic DNA from each species was analyzed in three 10-fold serial dilutions in triplicate reactions using the optimized 16 S qPCR conditions as described above.
Data analysis
For each species tested, reaction efficiency and correlation coefficient were calculated using the data from tests against three 10-fold serial dilutions and presented in Table
Additional file 3
Figure S3A-E. Amplification plots of the non-perfect match targets, including
Click here for file
Laboratory quantitative assay validation using pure plasmid standards and mixed templates
Assay quantitative validation
For the assay quantitative validation, we followed the Minimum Information for publication of Quantitative real-time PCR Experiments, or the MIQE guidelines whenever applicable
Data analysis
Using the data generated, the following assay parameters were calculated: 1) inter-run assay coefficient of variation (CoV) for copy number and Ct value, 2) average intra-run assay CoV for copy number and Ct. value, 3) assay dynamic range, 4) average reaction efficiency, and 5) correlation coefficient (
Additional file 4
Figure S4A-E. Coefficient of variance (CoV) distribution across assay dynamic range for mixed templates.
Click here for file
Additional file 5
Supplemental File 1. Detailed results for BactQuant using the stringent criteria.
Click here for file
Figure 2
A-B. Standard curve amplification profiles of the BactQuant assay generated from 10 μl and 5 μl reactions using seven ten-fold dilutions and normalized plasmid standards at 109copies/μl.
A-B. Standard curve amplification profiles of the BactQuant assay generated from 10 μl and 5 μl reactions using seven ten-fold dilutions and normalized plasmid standards at 109copies/μl. The Ct value of standard curve using 5 μl reaction volumes (Figure
Figure 3
Inter- and intra-run coefficient of variation (CoV) for 10 μl and 5 μl reactions using seven ten-fold dilutions and normalized plasmid standards at 109copies/μl calculated using data from multiple runs.
Inter- and intra-run coefficient of variation (CoV) for 10 μl and 5 μl reactions using seven ten-fold dilutions and normalized plasmid standards at 109copies/μl calculated using data from multiple runs. The data is presented for both copy number (
Bacteria-to-human ratio calculations
Calculations were performed using the following copy number and genome size estimates: the average bacterial 16 S rRNA gene copy number per genome was estimated to be 3.94 copies as calculated by rrnDB
Assay design and initial specificity check
Using our 16 S rRNA gene nucleotide distribution output, we identified a conserved 500 bp region for assay design. Within this region, we selected three highly conserved sub-regions abutting V3-V4 for the design of a TaqMan® quantitative real-time PCR (qPCR) assay (Additional file
Additional file 6
Supplemental File 2. Detailed results for BactQuant using the relaxed criteria.
Click here for file
Numerical and taxonomic
Using the stringent criterion,
On the genus- and species-levels, 1,778 genera (96.2%) and 74,725 species (83.5%) had at least one perfect match using the stringent criterion. This improved to 1,803 genera (97.7%) and 79,759 species (89.1%) when the relaxed criterion was applied (Table
During our
Additional file 7
Supplemental File 3. Detailed results for published assay using the stringent criteria.
Click here for file
Additional file 8
Supplemental File 4. Detailed results from published assay using the relaxed criteria.
Click here for file
Laboratory analysis of assay performance using diverse bacterial genomic DNA
Laboratory evaluation of the BactQuant assay showed 100% sensitivity against 101 species identified as perfect matches from the
Laboratory evaluation against the five
Laboratory quantitative assay validation using pure plasmid standards and mixed templates
To fully characterize the assay quantitative profile, the BactQuant assay was tested using different reaction volumes and against both pure and mixed templates containing bacterial and human rRNA gene targets. Laboratory evaluation using pure plasmid standards in 10 μl and 5 μl reaction volumes showed excellent amplification profiles, with an assay dynamic range of 102–108 16 S rRNA gene copies per reaction (Figure
Further laboratory evaluations using mixed templates showed that the ratio of bacteria-to-human DNA ratio determined the assay dynamic range of the BactQuant assay (Table
Additional file 9
Table S1. Base distribution output used in primer and probe design, with the bolded base signifying the selected base(s) and incorporation of more than one allele at a given nucleotide position was accomplished using degenerate bases. The alignment position information in the base distribution file contains many gaps as a result from the sequence alignment and differs from the
Click here for file
We designed and evaluated a new expanded-coverage bacterial quantitative real-time PCR assay targeting the 16 S rRNA gene. To accomplish this, we curated a set of high-quality 16 S rRNA gene sequences for assay design and evaluated the coverage of our primers and as a union (rather than as separate entities). In addition, we improved the quantitative capacity of our assay using a cloned plasmid standard. Our computational and laboratory analyses showed that BactQuant had superior
To allow researchers to determine whether BactQuant covers key organisms in their target community, we provided additional detailed OTU coverage information in the Supplemental Files. We have applied the logic that an OTU was covered if it contained at least one perfect match sequence in the
For most studies, the desired measurement of bacterial load is the number of cells rather than 16 S rRNA gene copy number; however, the 16 S rRNA gene copy number varies among bacterial species and even among strains
Exogenous bacterial DNA, particularly from biologically synthesized reagents such as Taq DNA polymerase are a known limitation for analyzing samples with low bacterial load
Our use of qPCR-quantified plasmid standards addressed a major limitation in the preparation of qPCR quantification standards. The conventional approach of quantifying bacterial genomic DNA or plasmid standards necessitates converting mass (i.e., nanograms per μl) to copy number (i.e., 108 copies per μl) and can introduce substantial error. Thus far, we have also successfully applied BactQuant to a diverse range of clinical specimens, including swab eluents, surgical specimens, and respiratory specimens, but we did not present these findings in this paper. To fully understand the likelihood of false negative results due to interference from human DNA or BactQuant's limit of detection will require additional evaluations.
In summary, we have developed and evaluated a new broad-coverage qPCR assay—BactQuant—for bacterial detection and quantification that showed concurrently improved assay coverage and favorable quantitative parameters. Laboratory tests showed that in vitro performance was even better than predicted in the
Competing interests
The authors have declared that no competing interests exist.
Authors' contributions
CML contributed to the overall study design, the acquisition, analysis, and interpretation of data, and drafting the manuscript, MA contributed to the bioinformatics portion of the study design and its implementation, SK participated in bioinformatics analysis and assay design, PRH and YTH both contributed to the acquisition and interpretation of laboratory data, PK conceived of the study and contributed to the overall study design, LBP contributed to the overall study design and helped to draft the manuscript. All authors read and approved the final manuscript.
We thank Lindsey Watson (growth and extraction of ATCC isolates and performance of quantitative real-time PCR), Jordan Buchhagen (cloning and sequence verification of cloned standards), Jolene Bowers (generation of the 16 S rRNA gene plasmid clones), and Tania Contente-Cuomo (performance of quantitative real-time PCR) at Translational Genomics Research Institute for their assistance with different aspects of the laboratory work presented in this manuscript.
This work was supported by the National Institutes of Health (R01AI087409-01A1, R15DE021194-01), the Department of Defense (W81XWH1010870), and the TGen Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
A renaissance for the pioneering 16S rRNA gene
Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories
Vaginal microbiome of reproductive-age women
Topographical and temporal diversity of the human skin microbiome
A core gut microbiome in obese and lean twins
Integrating high-throughput pyrosequencing and quantitative real-time PCR to analyze complex microbial communities
Cultivating the uncultured
Simultaneous amplification and detection of specific DNA sequences
Quantitative real-time RT-PCR–a perspective
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
The Ribosomal Database Project: improved alignments and new tools for rRNA analysis
Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB
OligoCalc: an online oligonucleotide properties calculator
Database indexing for production MegaBLAST searches
Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0
vegan: Community Ecology Package. R package version 2.0-2
rrnDB: documenting the number of rRNA and tRNA genes in bacteria and archaea
Ribosomal RNA gene dosage as a function of tissue and age for mouse and human
Finishing the euchromatic sequence of the human genome
Nuclear DNA content and genome size of trout and human
Frequent contamination of Taq polymerase with DNA
Two novel real-time reverse transcriptase PCR assays for rapid detection of bacterial contamination in platelet concentrates
Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16 S DNA by real-time-PCR
Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens
Taq polymerase contains bacterial DNA of unknown origin
rRNA operon copy number reflects ecological strategies of bacteria
Life history implications of rRNA gene copy number in Escherichia coli
Empirical testing of 16S rRNA gene PCR primer pairs reveals variance in target specificity and efficacy not suggested by in silico analysis
How quantitative is quantitative PCR with respect to cell counts?
Identification and elimination of DNA sequences in Taq DNA polymerase
Contamination and sensitivity issues with a real-time universal 16 S rRNA PCR
Removal of DNA contamination in polymerase chain reaction reagents by ultraviolet irradiation
Reagent decontamination to eliminate false-positives in Escherichia coli qPCR


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Fairfield Inn & Suites by Marriott Rochester West/Greece
Grid of 28 images
Get an overview of this hotel3-star Rochester hotel in Northwest Rochester with free breakfast
Popular property highlights
Located in Northwest Rochester, this hotel is 1.2 mi (2 km) from Mall at Greece Ridge and within 3 mi (5 km) of Kodak Center for Performing Arts and Maplewood Park. Genesee River's High Falls and Diplomat Banquet Center are also within 6 mi (10 km).
An indoor pool, a 24-hour fitness center, and a snack bar/deli are available at this smoke-free hotel. Free buffet breakfast, free WiFi in public areas, and free self parking are also provided. Other amenities include a spa tub, coffee/tea in a common area, and a business center.
Hotel Features
Room Amenities
All 70 rooms provide free WiFi and free wired Internet, TVs with cable channels, and conveniences like sofa beds and refrigerators. Microwaves, coffee makers, and hair dryers are among the other amenities available to guests.Information missing or incorrect? Tell us!Opens in a new window
Fairfield Inn & Suites by Marriott Rochester West/Greece
Hotel Amenities
Hotel Amenities
In addition to an indoor pool, Fairfield Inn & Suites by Marriott Rochester West/Greece provides a spa tub and a 24-hour fitness center. The hotel offers a snack bar/deli. Guests can enjoy a complimentary breakfast. Public areas are equipped with complimentary wired and wireless Internet access.
This 3-star property offers access to a business center and a meeting room. This business-friendly hotel also offers barbecue grills, a picnic area, and coffee/tea in a common area. Onsite self parking is complimentary.
Fairfield Inn & Suites by Marriott Rochester West/Greece is a smoke-free property.
- Total number of rooms - 70
- Number of floors - 3
- Barbecue grill(s)
- Business center
- Coffee/tea in common areas
- Free newspapers in lobby
- Free breakfast
- Dry cleaning/laundry service
- Express check-in
- Express check-out
- 24-hour fitness facilities
- Free WiFi
- Laundry facilities
- One meeting room
- Picnic area
- Safe-deposit box at front desk
- Snack bar/deli
- Free self parking
- Smoke-free property
- Spa tub
- Indoor pool
- Free wired high-speed Internet
Family Friendly Amenities
- Family Hotel
- Indoor pool
- Free Wi-Fi
- Snack bar/deli
- Laundry facilities
- Sofa bed
- Refrigerator
- Microwave
Available in all rooms: Free WiFi , Free wired high-speed Internet
Available in some public areas: Free WiFi , Free wired high-speed Internet
Free self parking
Room Amenities
- Premium TV channels
- Pay movies
- In-room climate control (air conditioning)
- Air conditioning
- Refrigerator
- Coffee/tea maker
- Microwave
- Daily housekeeping
- Hair dryer
- Iron/ironing board
- Desk
- Cable TV service
- Television
- Sofa bed
- Free WiFi
- Free wired high-speed Internet
Where to Eat
Guests are offered a complimentary hot/cold buffet breakfast.
Nearby Things to Do
An indoor pool and a spa tub are on site. Other recreational amenities include a 24-hour fitness center.
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
- Accessible bathroom
- In-room accessibility
- Roll-in shower
Hotel Policies
Check-in time ends at midnight
Check-in time starts at 3 PM
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Kids stay free! Up to 2 children 18 years old and younger stay free when using existing bedding.
- Pets not allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No rollaway/extra beds available
No cribs (infant beds) available
Hotel Name
- Fairfield Inn Marriott West/Greece Hotel Rochester
- Fairfield Inn Rochester West/Greece
- Fairfield Inn Marriott Rochester West/Greece Hotel
- Fairfield Inn Marriott West/Greece Hotel
- Fairfield Inn Marriott Rochester West/Greece
- Fairfield Inn Marriott West/Greece
We should mention
Up to 2 children 18 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.


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SailNet Community (http://www.sailnet.com/forums/)
- Gear & Maintenance (http://www.sailnet.com/forums/gear-maintenance/)
- - Marine LCD TV (http://www.sailnet.com/forums/gear-maintenance/100557-marine-lcd-tv.html)
Marine LCD TV
OK, so things have gotten fairly quiet here recently, and I thought I'd toss out a grenade. :)
More seriously, I have started entertaining the thought of putting in an LCD TV in our cabin. It would be great for my kids when we're at the boat and the summer afternoon showers roll in, trapping them inside. We have a small (7") portable DVD player, but it's hard for two of them to share that little screen, and that got me wondering if anyone has installed an LCD TV on their boat, and if so, what brand? Has it held up well in the "harsh marine environment"? I'm not looking for something super-huge, but a 20-24" TV could improve the over-all experience quite a bit for my boys.
Before anyone asks, my kids are avid readers (even though they are only 7 and 5, they can both read well) and we will keep the cabin stocked with good reading material for them. We'll also have playing cards, etc. But at their ages, their attention span, even for something like reading and cards, can be fairly short and being trapped in a cabin even for an hour can feel like an eternity (including for us adults). Having a TV as an option could significantly improve things.
I know I can get LCD TV's for about $200 in the size range that I want, and that's not a bad investment, and it's almost "disposable" at that point, but I'd really hate to buy one only to learn two months from now that the 120 degree cabin in August is/was likely to cause the liquid in the LCD to expand enough to rupture, or that the corrosion from the humid salt air in the cabin was enough to eat away at some of the electronics in only a few weeks.
Any ideas/suggestions are appreciated!
Re: Marine LCD TV
This is the TV we bought for our boat. It really helps with the grandkids and they only get to watch it as a reward. Works well, nothin wrong with bribery:)
Easy mount bracket
And add on this little wonder device, it has amazing reception: (and cheap)
Re: Marine LCD TV
+1 to kellysails.
We have the same setup in our boat. The only difference is we have a 22" Visio. Additionally, we have a feed located high in the anchor locker that we can hook direct to the pedestal in marinas that offer cable TV at the slip.
We've gone two seasons with the TV aboard without a problem.
Re: Marine LCD TV
If your kids are avid readers, why tempt them away from that? I'm pretty vehemently opposed to TV on a boat.. isn't the whole point to escape that 'world' along with it's gadgets and distractions? Beachcombing and swimming, boat handling, navigating, there's always something to do with a good book as a fall back.
Even when I try to read during the day I find myself distracted by the world around me.. a high-EQ anchoring exercise, a particularly nice boat entering the bay, a couple yelling at each other but in a way that only the rest of us can hear it all ;) and best of all, the sound of a happy child on the beach...
Who has time for TV???
jmo, as ever.....
Re: Marine LCD TV
[/IMG]Faster :)
I was wondering how long it would take to get that response. Sometime Grandpa (me) and the 4 and 7 year old are just too tuckered out after a long day of fun. We do read to them but there are times they just want to chill. Not always a bad thing. Everything in moderation (except wine, women, and song) as I say.
Re: Marine LCD TV
There are a number of 12 volt TVs sold to truckers and campers. You might want to check them out, but I've never heard of them. Finding a good antenna is a PIA. I took the TV off my boat because I could not get anything with the antenna I owned. Thought about hoisting it on the flag halyard a couple times, but never got that far.
Re: Marine LCD TV
Any normal TV will do fine, as long as it is not exposed to direct water, you will be fine.
No need to overthink this.
Want to run it off 12 volt, just buy a cheap inverter, them small TV's don't draw much.
Have had the same ol' LCD TV from what is equal to Wallmart over there, for years, no trouble so far.
Re: Marine LCD TV
I used my laptop with one of these jobbies:
Hauppauge Computer Works : WinTV-HVR-950Q Product Description
worked pretty good!
Re: Marine LCD TV
I can only offer this advice. I prefer a TV that has the standard four hole flush mount directly on the back of the TV, so that it can be mounted flush to a bulkhead. Some have attachments which have the four hole pattern and, therefore, stand off the wall. They get in the way and are less secure when underway, in my experience. View angles have improved dramatically, so adjusting angle should not be terribly necessary.
Many TVs are capable of running directly on 12v, but have the "brick" in the line that converts 110v AC to 12v DC. If you find one, you can connect it directly to your 12v system. However, this data is rarely published and you actually need to read the brick itself. Further, many seem to be moving toward 19v, which is more common for laptops, I'm told.
If you find a model that is both 12v and flush, please let me know. I hate our TV!
We only use our TV to show a movie from time to time, which is best done off an Ipad or Iphone having been downloaded and rented through iTunes. Streaming Netflix, etc, is a PITA. YMMV
Re: Marine LCD TV
As noted, the issue is the antenna, especially while cruising.
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August 20, 2009
Amber Rose With Hair
Here are photos of Kanye's girlfriend Amber Rose with hair, way before she made the dive towards shaving it all off. Either way she goes, she's sexy. But the short hair look definitely has hair killing the game right now. Click here to see her photos in Smooth Magazine earlier in the year.
CategoriesGirlsPhoto Of The Day


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This Gif entitled Parker n H was created from the youtube video 2 years ago. It uses 12 frames per second and is displayed at 240px resolution. This animated image plays for 4 seconds, was built with normal quality and an estimated weight of 209.53 KB for download. This animation was originally created from the GRASER BLOWS CHUNKS!!! video from 00:36 to 00:40.
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"Buenos dias! Me llamo Senor Axford. ¿Habla usted inglés?"
So far, this seems to sum up my travel adventure to Santiago, Chile (as well as the extent of my Spanish language skills). As many of you know, this is pretty much what I do: Fly to faraway places, talk to execs and IT Pros about information security. Lather, rinse, repeat. I'm in town this week to speak to meet some of the local Microsoft security people, talk to customers, and do some press interviews, and deliver the really fun session "Identifying Computer Attacks: Tips, Tricks, and Tools" to the local IT Pros in Santiago on March 13th. I wish I was staying to see some of the sites (aka snowboarding), but as the Bob Seger song goes "There I go, on the road again…here I am, up on the stage….."
I do love my job. I love seeing the "light bulb" come on when I explain to them that security isn't about expensive products and fancy consultants. I especially love seeing what it is you guys are doing. You're the ones who are reducing risk and changing the threat landscape. As I told some guys down in Houston last week, the problems we face as security pros is global. Wicked Rose and his gang from NCPH (great webcast BTW!) isn't just attacking one U.S. business. Data Leakage isn't just a concern in Berlin, and Bot infections aren't just happening in Santiago. I love hearing how you guys are beating back the Horde. We don't always tell you this….but you are doing a great job!
…and just so you don't think that your speakers at Microsoft are sitting back….check out the work Keith Combs and U.S. TechNet Team has going on for Longhorn Server starting on April 1st. He's trying to juggle everything. Steve Riley is also out in Asia this week doing more security events. We work hard, so you guys get the latest info!
OK, it's time for a siesta. I'll try and post a little bit each day as I enjoy the hospitality of Chile!


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