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70cl / 55.1%
1965 32 Year Old
- Highland Single Malt Scotch Whisky
- Scotch Malt Whisky Society
FREE UK standard delivery
£1,200 £1,000 ex VAT
- In Stock
- Express delivery available
The 7th cask that The Scotch Malt Whisky Society bottled from Glenugie. This was bottled in 1997, fourterrn years after the distillery closed. Add this to the fact that no official bottlings were ever released, this is a much sought after and hard to find single malt.
Please note that this is an old bottle and the closure may have deteriorated; care should be taken when opening the bottle. As such, the item is sold as described.


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NZD/USD: Murray levels analysis
D1. It seems the level of 4/8(0.8301) can be a serious obstacle for the bears, judging by the fact that they have consistently failed to break below it. Therefore, we consider the most likely the scenario of a rebound from 4/8to 5/8(0.8423), and in case of breakdown of the latter – to CTW1.
H4. Stop at the level of 0/8 (0.8301) in the near future may lead to the correction of the pair to the area STD1. Break below 0/8 and fixing below this level will signal the fall to -2/8 (0.8180). For now the recoil to the level of 7/8(0.8362) and STD1 looks more likely.

subset: commoncrawl


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Spice Williams-Crosby
4,800pages onAdd New Page
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This article is considered a stub because it is lacking information and is thereby short. You can help the Charmed Wiki by expanding it.
Spice Williams-Crosby née Kneuer, born April 26, 1952, North Hollywood, Los Angeles, is an American actress, and stunt performer. She portrayed Hulk Phoebe in the Season 8 episode "Hulkus Pocus".


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Re: Looking for the grave of Mr. John W. McPeek
In reply to:
Looking for the grave of Mr. John W. McPeek
Silvio Passalalpi 7/24/13
According to his death certificate, John Weston McPeek was born on 11 March 1915, the son of Otto McPeek and Marguerette Jones.He died of a coronary occlusion at Parkersburg, Wood county, West Virginia, on 23 January 1970, and is buried at Mt. Olivet Cemetery in Parkersburg.He worked in the railroad industry.When he died, he was married to Wanda Tennant.His death certificate indicates World War II service.A copy of the death certificate is here:http://www.wvculture.org/vrr/va_view2.aspx?FilmNumber=1992293&ImageNumber=1266http://www.wvculture.org/vrr/va_view2.aspx?FilmNumber=1992293&ImageNumber=1266
John Weston McPeek married Wanda Voncielle Tennant on 29 June 1963 at Parkersburg, when he was 48 years old.His parents are listed as O.J. McPeek and Margaret May McPeek.A copy of the marriage record is here:http://www.wvculture.org/vrr/va_view2.aspx?FilmNumber=580576&ImageNumber=362http://www.wvculture.org/vrr/va_view2.aspx?FilmNumber=580576&ImageNumber=362
John W. McPeek's enlistment information for World War II service is here:https://familysearch.org/pal:/MM9.1.1/K8PQ-Y1F
However, the enlistment information is just an excerpt, not an original document.
More Replies:
Re: Looking for the grave of Mr. John W. McPeek
Silvio Passalalpi 7/26/13


text: UPDATED 12:06 9th May 2016 SPONSORS: Home: Sands Civil & Structural Chartered Engineers Away: Sands Civil & Structural Chartered Engineers Training: AVAILABLE Special: AVAILABLEGPS: Exeter City Joel Grant joined Exeter City on August 21, 2015, having left Yeovil Town that summer following their relegation from League One.Born in Acton, England, Joel started his career off in the Watford youth set up and made 10 appearances for the Hornets in the 2005/06 season as they were promoted to the Premier League.However, he found first team opportunities hard to come by in England's top division and went out on loan to Aldershot before making a permanent switch to Recreation Ground. He scored six goals across a season and a half with the Shots as they won the Conference Premier and Conference League Cup in 2007/08. Joel then joined Crewe Alexandra that summer. He would score 16 goals in more than a 100 appearances for the Alex before joining Wycombe Wanderers on a free transfer in summer 2011 and Yeovil Town in June 2013. Joel is a full Jamaican international. He scored his first goal for Jamaica in a 2–2 draw with Egypt on June 4, 2014 and also netted a header against South American champions Chile in the summer which earned him a call up to the Reggae Boyz' Copa America 2016 squad.


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A multistate molecular approach to the proton-hydrogen collision is formulated in terms of an impact parameter perturbed stationary-states approximation. Spurious long range couplings are avoided and Galilean invariance is enforced by the inclusion of momentum translation factors which are determined variationally within an Euler-Lagrange formalism (Crothers & Hughes 1978). Well defined radial and rotational coupling matrix elements are employed in the 1-7 keV impact energy range in a six-state (1s<latex>$\sigma _{\text{g}}$</latex>, 2p<latex>$\sigma _{\text{u}}$</latex>, 2p<latex>$\pi _{\text{u}}$</latex>, 3p<latex>$\sigma _{\text{u}}$</latex>, 3p<latex>$\pi _{\text{u}}$</latex>, 4f<latex>$\sigma _{\text{u}}$</latex>) calculation of elastic and inelastic differential scattering cross sections, charge exchange probabilities and both direct and exchange H(2p) production total cross sections. They are also employed in the same energy range in a ten-state (1s<latex>$\sigma _{\text{g}}$</latex>, 2p<latex>$\sigma _{\text{u}}$</latex>, 3d<latex>$\pi _{\text{g}}$</latex>, 2p<latex>$\pi _{\text{u}}$</latex>, 2s<latex>$\sigma _{\text{g}}$</latex>, 3p<latex>$\sigma _{\text{u}}$</latex>, 3d<latex>$\sigma _{\text{g}}$</latex>, 4f<latex>$\sigma _{\text{u}}$</latex>, 4d<latex>$\pi _{\text{g}}$</latex>, 3p<latex>$\pi _{\text{u}}$</latex>) calculation of both direct and exchange H(2s) production total cross sections. The results are in excellent accord with experimental data and show considerable improvement on previous molecular calculations. This success is attributed to the inclusion of both momentum translation factors and radial coupling matrix elements.
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OD's sports vision training helps ballplayerSeptember 13, 2011
Outfielder Bryce Harper is being widely heralded as baseball's next great star. With perhaps unprecedented natural ability (as well a brash and colorful personality), many believe he could become a major sports phenomenon, on a par with Tiger Woods, Lance Armstrong, or Michael Jordan.
If he does, he will almost certainly focus new attention on the importance of good eyesight for athletes, according to Keith Smithson, O.D., team optometrist for the Washington Nationals.
An eye examination, contact lenses, and sports vision training provided by Dr. Smithson this past spring are being credited with re-charging the young power hitter's career just as it appeared to stalling.
Harper was the first pick in the 2010 Major League Baseball (MLB) Draft. The Nationals gave the 18-year-old, Las Vegas native a highly publicized, five-year, $9.9 million contract. Sports Illustrated featured Harper on its cover, proclaiming him to potentially be among the best players ever to pick up a bat. ESPN noted that Harper had dominated high school and college ball like no other player in decades. He was designated the "top prospect" for baseball stardom by the Web site Baseball America and other media with such rankings.
However, Harper's minor league career got off to an unexpectedly slow start. His statistics after joining the Nationals' AA farm team, the Hagerstown (Md.) Suns, were unimpressive. Harper was batting just batting just .231. Sportswriters described his performance as "uneven" and "nondescript."
Time magazine questioned whether Harper would live up to his highly touted potential.
Then, in April, Nationals coaches sent Harper to Dr. Smithson for an eye examination. To both the ballplayer's and the optometrist's surprise, fairly serious refractive error was diagnosed.
Dr. Smithson prescribed Harper a set of contact lenses to correct acuity. He also initiated a comprehensive vision training program to strengthen Harper's eye muscles and allow him to process what he sees more quickly.
The night after the examination, Harper hit a double and a single. The next night, he homered. The night after that, he singled, doubled, homered and drove in six runs. In 20 games following his visit to Dr. Smithson, Harper hit .480 (36 for 75) with a .547 on-base percentage and an .893 slugging percentage — with 7 homers, 10 doubles and 23 RBIs.
In July, Harper was promoted to the Nationals' A league affiliate, the Harrisburg (Pa.) Senators. As this AOA News went to press, his batting average stood at .304 and he had just recorded his first A league homerun.
"It was like I was seeing in HD," Harper said after being fitted with his new contact lenses. During one interview, Harper stated that, in retrospect, he felt he had been "blind as a bat" prior to seeing the optometrist.
During lectures on sports vision at optometric meetings, Dr. Smithson notes that such reactions are not uncommon among athletes following sports vision care.
Because of the great demands placed on their vision, athletes often find that even relatively minor correction results in a very substantial increase in quality of vision, Dr. Smithson says.
Dr. Smithson attempts to correct all of his baseball playing patients to average acuity levels found in MLB players in a study by Daniel M. Laby, M.D., of the Harvard Medical School and David G. Kirschen, O.D., of the University of Southern California based on a study of MLB players.
Correction to the average acuity found in the major leagues often does not require a strong prescription but often does result in a very satisfied patient-athlete, Dr. Smithson noted during one recent lecture.
"In baseball, acuity is so critical that it can certainly intensify the effects of correcting just about any level of refractive error," Dr. Smithson said. "Many cases, prior to a good sport vision examination, players simply do not have the acuity needed to maximize their performance at the highest level."
"It was not until he was faced with the competition of professional league play that he realized he was not seeing the ball as well as he should," Washington Post sportswriter Dave Sheinin observed in a feature article on the eye examination and Harper's subsequent improvement in play.
Harper had once tried contact lenses and given them up.
"I needed (the contacts) in college," he said. "But I tried them for a while in high school, and they gave me headaches really bad. So I just got by without them. But these are a new kind (of lenses), and they really help. The difference (in vision) is huge."
Harper has now effectively become America's best-known example of a previously unsuccessful contact lens wearer who has been reintroduced to the lenses successfully thanks to new lens materials and designs, Dr. Smithson noted.
Harper's rapid improvement was not surprising, Dr. Smithson observed. In addition to the immediate improvement in visual acuity provided by the lenses, Harper likely saw improvement in his visual processing skills quickly, he said.
"Studies tell us that vision training, after as little as two sessions, can show statistical improvement," Dr. Smithson said. "It's a pretty dramatic improvement, pretty quick with certain things."
It is impossible to say exactly how much of Harper's on-field improvement can be attributed to the lenses and how much is the result of the vision training, Dr. Smithson said.
Contact lenses and vision training are not the sole reasons for Harper's improvement on the playing field this year. The young outfielder continues to benefit from professional coaching, physical training, and experience against more highly skilled players, baseball writers note. Nor has it meant the end of his problems on the diamond. Over recent weeks, Harper's performance has again reached a plateau in at least some respects.
However, the sports media almost universally expects Harper to move on to a highly successful career in the major leagues.
As he does, Dr. Smithson believes, demand for proper vision correct and vision training among athletes – and the general public – is likely to increase.
The eye examination and Harper's subsequent on-field improvement have already the subject of coverage in Washington-area media and baseball Web sites.
Dr. Smithson is the sports vision specialist for Northern Virginia Doctors of Optometry, a 13-practitioner practice with six offices in the suburbs south of the nation's capital.
In addition to the Nationals, Dr. Smithson is the team optometrist for the Washington Wizards of the National Basketball Association and the DC United major league soccer team.
He has also served as a sports vision consultant for the Baltimore Ravens of the National Football League, Washington Freedom professional soccer club, Washington Mystics of the Women's National Basketball Association, and Nike.
However, virtually any practice can provide at least some sports vision services and should be prepared to do so, Dr. Smithson believes.
Dr. Smithson is a member of the AOA Sports Vision Section, which offers advice on sports vision practice and information on various sports vision programs through its AOA Web site page (www.aoa.org/x4787.xml).
The AOA Web site also offers information on sports-related vision issues for the general public on its Sports & Vision page (http://www.aoa.org/x5277.xml).
How to Get Started in Sports Optometry, an article on the introduction of sports vision services in an optometric office and the sports vision acuity standards developed by Dr. Laby and Dr. Kirschen, appears in the August edition of Optometry: Journal of the American Optometric Association.


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text:
Peace and QuietMature
His heart jumped for joy and his dark eyes lit up with a bright smile at her sudden excitement to play. While he had heard the phrase, "these aren't toys," numerous times before, from those who didn't dabble in the BDSM, nor the med fetish lifestyle, it was him who had deemed them toys. Not only for his own pleasure, but the pleasure of his girls as well. He had a lot of medical equipment and not just in his car in case of emergencies, but in the manor. As long as he could keep an eye on Scarlett and supervise her as she played with his "toys," he knew he had nothing to worry about.
He turned his head to plant a kiss to the top of her head, giving her hand a squeeze before pulling away. "Alright then. Watch out, love," he said, turning to reach behind her seat and pull out his big black doctor bag, setting it between the seats. "Here you are, go wild little one. See what you find in there. There's a tank of oxygen and nitrous oxide back there as well."


text:
Originally posted on Celtics Town | Last updated 3/5/13
Doc Rivers feels for Philadelphia.As we wait for a REAL LIVE BOSTON CELTICS BASKETBALL GAME HOLY CRAP YOU GUYS, CSNPhilly.com brings us this nugget from Doc Rivers on Philly's Andrew Bynum predicament:"It cripples your franchise," said Rivers. "It really does, when you have a guy like that. I had Grant Hill for three years. We made the playoffs every year with that group. But, I mean, it was hard."What was hard was … you go into the year with your offense and defensive schemes for Grant and for Bynum, and then you're running half of it, but you don't want to change too much, because if he comes back, then you're going to have to change back. So, you just felt like you were caught in a flux the entire season. That's how I felt."No matter how you feel about Philadelphia (and it would be understandable if you didn't feel very friendly toward them after a hard-fought playoff series and repeated meetings in the regular season), it's hard not to feel bad for their current situation, especially since the Celtics found themselves in a similar state of limbo after Rondo went down for the season. The Sixers dealt Andre Iguodala and got what they assumed would be a franchise center, and it was a deal widely praised around the league. But Bynum is injury-prone, and this season has been no exception, which puts Philly in a bind. Do they build their offense around Bynum without knowing if he will return next year? Do they build around first-time all-star Jrue Holiday and risk alienating Bynum, which would mean they gave away Andre Iguodala for nothing? It's a difficult decision, and I don't envy Philadelphia's management. Sure, they knew the risks with Bynum, but that doesn't make it any less unfortunate for them that the risks turned out to be reality.Also worth noting: Doc Rivers played against guys who are still in the league, which is a total trip to me.Follow Tom on Twitter: @Tom_NBA.
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text:
Posted May 22, 2014 by BlueMagic in Music Video
Lil Terio Drops His First Music Video on VEVO [Video]
From Vine to VEVO, Terio has dropped his first music video. No, he's not rapping in this video but Polo, KayLuv, and Kidd Willie has taken that burden for him. Terio doesn't really speak at all in the video but it looks like the kid is enjoying life right now. Check out the video below and make sure to leave us your thoughts. Ooohh Kill Em!


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Mobile App
Assisi School of Nursing
Total Students
Fees Range
How to reach
Assisi School of Nursing is situated at Propose P.O Erumely , Kottayam, Kerala, India -686509 in Kottayam.
Courses Offered
- 42 Months
- General Nursing & Midwifery-G.N.M.
- Medicine / Beauty / Health Care
Assisi School of Nursing - ASN
We Care, God Cures. Healing Ministry in the Church is purely the continuation of mission of Christ Jesus, the Healer. The modern Nursing as noted by Florence Nightingale is a noble Profession, a call and vocation to be the eyes of the blind, the ear of the deaf, the voice of the dump and the hand of the handicapped.Assisi School of Nursing is developing the young women into well integrated nursing personnel inculcated with both Christian and humanitarian values. The school of Nursing started in the year 2005 was functioning in the hospital annex for three years. In 2008, October, the school was shifted to the new school building. The school is blessed with sufficient physical facilities and infrastructure as per INC requirements. The class rooms are spacious enough to accommodate 30 students. Laboratory facilities are provided for Fundamentals of Nursing, Community health Nursing, Nutrition, Obstetrical Nursing, Pediatric Nursing and computer lab. AV Aid room and Anatomy lab are well equipped with necessary materials and specimen. Our library is having more than 1000 books with adequate seating arrangements.The School of Nursing is recognized by both Indian Nursing Council and Kerala Nurses and Midwives Council. The Inspectors have expressed their great appreciation in the infrastructure of the school specially the hostel block. The students are provided with enormous living facilities in the hostel. Two students are occupying a room. All facilities are provided in the hostel like washing and ironing, play – ground, recreation hall etc. The hostel is managed by the religious sisters.A well equipped hostel managed by sisters is available for the students.
Why join Assisi School of Nursing
The college's infrastructure is well furnished with all modern facilities.
1 Type Courses Available
- 1 UG Courses
Assisi School of Nursing Placements
Visiting Companies
Student Placement
At Assisi School of Nursing (ASN) the students are endowed with all convenient amenities that make their life 'go easy' in college hours, alongside make learning more interesting. Some of the facilities include well ventilated classrooms, recreational areas, hostel facility for boys and girls, indoor and outdoor games, stocked library, seamless internet/wifi connectivity, medical facilities etc.
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Hunger Project
November 6, 2016 | CFE Arena
UCF Panhellenic Council and Feeding Children Everywhere are partnering to package 250,000 healthy meals for local hungry children. Together, with the help of the UCF community, we have had the opportunity to assemble and distribute 432,544 healthy meals to families in the Central Florida community. Gather your friends and fellow Knights to join us in packaging 250,000 healthy meals during our 5th annual Pack to Attack Hunger Project on November 6th!


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Sanaa, Feb 16 (AFP) Police shot and killed a protesterin Yemen''s main southern city of Aden, medics said, whilestreet unrest in the capital Sanaa against President AliAbdullah Saleh flared for a fourth straight day.
The unidentified fatality was among four woundedpeople taken to hospital in Aden, where demonstrators hurledstones at police, set tyres and vehicles on fire and stormed amunicipal building where heavy gunfire was heard.
Medics said the victim had been hit in the back.
Security forces, heavily deployed in Aden, arrested atleast four people as they fired warning shots and tear gas todisperse protesters who had gathered at the Al-Ruweishat busstation in the Al-Mansura neighbourhood of Aden.
Chanting "the people want to overthrow the regime" and "it''stime to leave, Ali," the protesters represented the angerswelling in Yemen, the poorest Arab state, strategicallylocated at the southwest corner of the Arabian peninsula.
In the capital Sanaa, at least 10 protesters were hurtamid clashes between students demanding the ouster ofPresident Ali Abdullah Saleh and supporters of his rulingGeneral People''s Congress.
Hundreds of students had set off for Al-Sabiine squarenear the presidential palace, only to be attacked by a likenumber of Saleh loyalists armed with batons, stones anddaggers.
The protesters -- inspired by the uprisings in Egyptand Tunisia -- responded by hurling stones, and when theviolence spread into the campus of Sanaa university, where themarch began, police fired warning shots.
"The thugs and supporters of the ruling party...wantto massacre" the students, the head of the university''sstudent union, Radwan Masud, told AFP, adding that 10 studentshad been hurt.
He vowed that the students would "continue theirrevolt and will not be hindered by the ruling party''sactions."
Elsewhere in Sanaa, a sit-in by judges demandinggreater independence for the judiciary and the sacking of theentire Supreme Judicial Council, including the justiceminister, went into its second day outside the justiceministry.
The judges, who have poured into Sanaa from all overYemen, also want higher salaries.
In other protests, workers in Sanaa gathered atseveral state-owned companies to demand that their managers tostep down. They too also called for higher wages.
Police in Sanaa stepped in when supporters andopponents of the president clashed yesterday, leaving threeinjured. In Taez, south of the capital, the two sides alsoclashed.
On Monday, rocks and batons flew in the capital asprotesters -- mainly students and lawyers -- confronted policeand Saleh''s supporters. Police also clashed with around 2,000protesters in Sanaa on Sunday.
In the face of the unrest, Saleh has postponed a visitto the United States that had been planned for later thismonth, after the opposition agreed on Sunday to resume talkssuspended since October. (AFP)


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Saturday, August 13, 2011
The Wedding Shroud by Elisabeth Storrs now available worldwide
Posted by Tracy Falbe at 6:21 PM
The Wedding Shroud by Elisabeth Storrs of Australia, I'm pleased to pass along her announcement that it is now an ebook available to readers in many countries.
When I reviewed The Wedding Shroud last December it was only available in the Australian market. Her carefully crafted, unique, and elegantly written novel about the arranged marriage of an ancient Roman woman and an Etruscan lord can definitely be enjoyed by lovers of historical fiction regardless of where they live.
This novel really made me contemplate the world of ancient people and how it differed from our modern world. The primitive elements and the superstitions interwoven with an advanced culture of music, cuisine, language, law, mathematics, and architecture were fascinating. The characters were complex, tormented, and I was never quite sure what anyone would do.
Look for The Wedding Shroud at Amazon, Amazon UK, and the iBookstore.
Related article: Mysteries of Etruscan civilization.
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text: A pilot factorial randomised cohort trial of manual therapy or acupuncture for low back painVivienne C Dascanio, Yvonne Birks, David Torgerson2011Background Randomised control clinical trials of acupuncture have been hampered by the challenges of assessing it as a complex intervention. Controlling for and separating placebo effects whilst identifying its efficacy as a treatment can be difficult [1]. The comparison of acupuncture to other complex interventions has been recommended to assess the… (More)


text:
Vilmos Zsigmond
Z Channel: A Magnificent Obsession
Founded in 1974 and based in Los Angeles, the Z Channel was the nation's first pay-cable station, preceding HBO or Showtime. At its pinnacle in the early 1980s, it served only 100,000 viewers, but nonetheless inspired and influenced the movie industry with its eclectic fare, securing a unique place in film history. IFC Films and director Xan Cassavetes celebrate the Z Channel and its founder, Jerry Harvey, with this captivating documentary.


text:
- Member since Jun 20, 2012
- Male / 33
- San Diego , California
- 14 Followers
- 0 Trailforks Points
yeti951SD laurenjenkins's article
Feb 9, 2017 at 13:03Feb 9, 2017
A Year of Change: Manon Carpenter Interview
@nug12182: yeah because nobody wants legal weed, amazing Mexican food, tons of amazing breweries, bigger mountains with better snow.
yeti951SD mikelevy's article
Aug 17, 2016 at 20:42Aug 17, 2016
Now THAT Was a Bike - Wade's Retro Maiden - Crankworx Whistler 2016
Should have put 26" wheels on it, now that would be nostalgic!
yeti951SD pinkbikeaudience's article
Aug 4, 2016 at 18:55Aug 4, 2016
Timed Training Results - Mont-Sainte-Anne DH World Cup 2016
@bentown: he said he's perfected the tuck to keep up with the fatties!
yeti951SD fraktiv's article
Aug 3, 2016 at 7:39Aug 3, 2016
Your Essential Guide to Mont-Sainte-Anne DH World Cup 2016
About time for another WC race! At least there was national championships to act as an appetizer to our DH cravings. Pinkbike was getting full of 29er and enduro crap again! I'm ready for some 27.5 racing (see what I did there? It's like accepting an annoying in law as family!)
yeti951SD pinkbikeaudience's article
Jul 11, 2016 at 23:41Jul 11, 2016
Team Videos - Lenzerheide DH World Cup 2016
Aaron Gwin class act! Danny Hart finally got the monkey off his back!
yeti951SD lunatyk's article
Jul 7, 2016 at 16:39Jul 7, 2016
Ask The Pros: World Cup DH vs. FMB Dirt Jumping - Video
You can ride dj for waaaaaaay less money than all the gear/bike you need for dh. Plus you need to be able to ride whatever is the latest wheel/hub/shock size standard and that can get expensive! DJ=26"x135 always has always will.
yeti951SD vernonfelton's article
Jul 5, 2016 at 9:21Jul 5, 2016
Kona Launches New 2017 Models
@lawnweenies1: not as long as there is a dirt jumping/4x/dual slalom/slopestyle
yeti951SD vernonfelton's article
May 13, 2016 at 8:20May 13, 2016
Five Ten Freerider ELC - Review
@jonmoss: the bike industry got the idea of changing standards from Five Ten. They figured just make a standard obsolete every few years so the consumer feels like they have to buy something to stay current.
yeti951SD paulaston's article
May 6, 2016 at 1:52May 6, 2016
Check Out - May
you get what you pay for in anti-chafe cream and under $300 taint gonna do it! Only thing that really works is if it has at least 20% unicorn blood. How much you might ask? You thought your carbon 650b 'duro frame was expensive!
yeti951SD scottsecco's article
May 4, 2016 at 12:50May 4, 2016
MUST WATCH - Dean Tennant: Higher Calling 2 - Video
@taquitos: he has a special rack to carry many leather bound books.
Photo by yeti951SD


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'Do you like Kipling?" asks the colonel on the seaside postcard. "I don't know, I've never Kippled," replies the shopgirl. But most people had, and they made him one of the most popular writers in the land. Remembered mainly for children's fables, he developed an image problem that kept his adult work off the radar for 60 years. The knee-jerk reaction is that he's a celebrator of British imperialism at its worst, so it's easy to overlook a few balancing facts.
Joseph Kipling was born in Bombay in 1865 and moved to England as a child. "Rudyard" was a middle name, after the lake in Staffordshire where his parents courted. The first cousin of the Conservative PM Stanley Baldwin, Kipling considered himself Anglo-Indian, which was common among the British born in India, and he returned to Bombay at 16. Starting work in a newspaper, he set a frenetic pace of writing, producing six volumes of short stories before heading to London, then the US, where he wrote the two Jungle Books. Although he loved Vermont, he made his home in Devon, and later in Sussex, after the growing anti-British sentiment in the US forced his hand.
Even before Queen Victoria had gone, Kipling was being seen as an arch-imperialist with such poems as "The White Man's Burden", although his writings contained ironies, particularly in Stalky & Co, about arrogantly cynical schoolboys.
The patriotic new century saw a leap in popularity. A reflection of his time, Kipling's admiration of India was undeniable. In 1907, he was awarded the Nobel Prize for Literature, becoming the first and youngest English language winner, but there was a troubling side to his work. Staunchly anti-Home Rule for Ireland and anti-Bolshevik, he supported the coming world war and secured a position for his son John in the Irish Guards. But the boy was killed in action, after which Kipling wrote: "If any question why we died/ Tell them, because our fathers lied."
He repeatedly turned down a knighthood, was lauded by Henry James and beloved by millions (his poem "If ..." still tops the charts) but times had changed. In later years Kipling's reputation shifted from innovator to old guard, partly because the gold swastika adorning his early books had become stigmatised; Kipling ordered the ancient symbol's removal and condemned the Nazis as early as 1935.
Now, both in England and India, reformation is under way. What cannot be denied is a facility for language that frequently surprises and dazzles, ripe for rediscovery.
- More about:
- Mumbai


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1.15 acres in Erath County, Texas
Beautiful lot in Mountain Lakes! Lake view, across the street from community pool and boat ramp. Lot 645 also available but not currently listed.
See GPS The address is Blue Marlin Ct.
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Contact the Seller for more information.
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As the backdoor commitee carried out its part of the mission after it opened the door, the frontdoor commitee was faced with the task of incapacitating the goon that Pheonix and Bugger were distracting. This was accomplished with a swift and well-placed kick to a very sensitive area of the goon, who crumpled crying and moaning in pain to the wood deck. Before he even had the chance to hit the ground, Pheonix and Bugger were inside the house with Jack, Derek and Penelope close behind.
Bugger, the first one in the door, rushed in and came to a screeching halt as his entry was met by a 9 mm pistol.
"Intru..." The goon behind the pistol began, however, he didn't get to finish his warning before he was tackled by both Scorch and Digger.
"You ok?" Spockeh asked as he rushed to them from the kitchen/back door area. At the same time, Scorch delivered a quick series of punches which knocked out the goon that he had tackled.
"A little shaken, but not stirred." Bugger replied. He bent down and picked up the 9 mm pistol. "Anyone object?" He asked. Spockeh shook his head.
"I don't want this to turn into a turkey shoot bud. We're in enough trouble as it is." He replied. Bugger nodded, ceeding to his advice, and stored it, with the safety on, in the waistband of his jeans.
"Ok guys, let's split up," Spockeh said, "Digger, you, Trucker and Scorch check the upstairs for anything interesting. If you find anymore weapons, either disable them or 'appropriate' them for us." The trio nodded then hurred off towards the stairs. Pheonix, Bugger, Derek check this floor for anything that we can use, radios, weapons, flashlights, anything." That trio dispersed and moved about looking for any useful equipment. With those 6 taken care of, that left, "Jack, you, Penelope, Ninja and I will take the basement. We might find something of more interest down there."
"What's the chances of the rest of the enemy hearing all of this?" Jack asked as they moved back to the kitchen area which held the stairs down to the basement.
"With all the action that's been going on, probably good. So our surprise is going to be lessened as time goes on." Spockeh replied. They reached the stairs and paused. "Ready?" Spockeh inquired. Ninja nodded, so did Penelope and Jack. "3...2...1... go go go!" As he opened the door, they were met with the sight of a goon that was about to open the door from the other side. He stood there confused and surprised, as did the goon who stared dumbly into Spockeh.
"Uh, who are you..." The goon began. However, his question was cut off as Spockeh delivered a swift kick to the man's mid-section which sent him tumbling down the stairs landing with a slightly muffled, and hollow, thud at the bottom.
"Get off my Sparta," Spockeh commented as he proceeded to fly down the stairs into the basement with Ninja, Jack and Penelope in tow. As his small team spread out in the basement he noticed only one door that occupied the basement, which surprised him. He expected more due to the size of the house they were in.
"Hang on guys, let's have the others catch up first." Spockeh said. The group nodded and waited, and while they waited, Spockeh was hoping that the delay wouldn't turn their Search and Rescue Op into a CASEVAC. Soon enough, the rest of the group arrived with Digger and Trucker bringing Grond with them.
"What'd you find?" Spockeh asked. Scorch held up a hand-held radio and a couple of flashlights.
"Ain't much, but they'll help." He said as he tossed the radios he and Derek were carrying.
"Anything else?" Spockeh inquired. Derek shook his head.
"Top floor's a bust," He replied.
"Grond hungry," Digger commented with a smile as he and Trucker positioned themselves near the door. He paused a moment, looked at Spockeh for confermation, which he gave, then breached the door with a quick and powerful crash.
Hang on Dru, we're coming. Spockeh thought as Scorch, never one for subilty, cried "House keeping!" then rushed though the door with the others in tow.


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The latest tablet-laptop hybrid from Asus is also one of the cheapest examples we've seen from this growing corner of the laptop market – and that £350 price is key to the T200TA's appeal.
It's the sort of low price that undercuts the cash you'd have to spend to get a proper tablet and a decent laptop, and that makes the T200TA a tempting option – as long as it can avoid common hybrid issues.
SEE ALSO: Best Cheap Tablets Round-up
Asus has been making hybrid machines for longer than almost anyone, and it shows. The T200TA doesn't look or feel like a £350 device: the tablet portion is coated with smart plastic that's got a subtle ringed pattern, and the keyboard half is topped with a metallic material that tapers to a narrow edge. That keyboard section has great strength, and the tablet half isn't much weaker – we'd have no qualms about slinging this machine in a bag without any protection.
The T200TA looks smart and feels strong, but its dimensions are a mixed bag – there clearly hasn't been much room left in the budget for slimming down.
Each half of the T200TA weighs just over 750g, with a combined weight of 1.54kg. Those figures trade blows with the competition: more than the Lenovo IdeaPad Yoga 2 11 and the HP Pavilion x360, but lighter than the 2.1kg bulk of the detachable Toshiba Satellite Click 2 L30W. It's also heavier than its predecessor – the Transformer Book T100 weighed just 1.2kg.
SEE ALSO: Best Free Antivirus: Windows Defender vs AVG, Avast & Avira
The T200TA isn't as slim as it looks. The tapered metallic material on the base sits on top of a thicker, darker section, and it's not the first time we've spotted Asus using this trick to make its laptops look slimmer.
The tablet section is 12mm thick, and the dock is 21mm thick so, combined, the T200TA is 33mm at its chunkiest point. That doesn't compare well to its rivals, as every other machine we've mentioned is less than 25mm thick.
The Asus' detachable 11.6-inch screen means the T200TA is easier to use as a tablet than most of its rivals – the HP and Lenovo machines don't detach, and the Toshiba's tablet half is thick and heavy. That helps this machine prove pretty manageable, but it's still not a clean bill of health for this hybrid: when its two sections are combined it's no thinner or lighter than dedicated laptops, and the tablet portion is still larger and heavier than the latest iPad.
The base unit serves up a USB 3.0 port, one USB 2.0 and a Gigabit Ethernet socket. The tablet offers better connectivity: a Micro HDMI connector, an SD card slot, a Micro USB socket and a headphone jack.
The buttons are all on the back of the tablet, with the Windows key, power button and volume rocker clustered into the top-left corner. Asus has ditched the Windows logo on the front of the tablet section – it was there on the previous generation of Transformer, but it wasn't an actual Windows button and simply proved confusing.
On the inside there's Gigabit Ethernet, Bluetooth 4.0 and dual-band 802.11n wireless, but no sign of 802.11ac.
SEE ALSO: Best Laptops, Ultrabooks and Hybrids
The Transformer has a 1366 x 768 touchscreen, which is what we expect on budget machines – but it's too low for Full HD movies or proper multitasking.
The Asus got off to a good start with a measured brightness of 307 nits. That's more than enough for indoor viewing, and it's good enough for outside use, too. It's brighter than the HP, but Lenovo's IdeaPad 2 11 was brighter still.
The Lenovo may be brighter, but the Asus machine fought back with better contrast and black levels of 1059:1 and 0.29 respectively. That means colours will appear richer and darker tones will be deeper. It's aided by good viewing angles, too – barely any brightness is lost when the screen is titled away.
The Asus's screen is bright and with good contrast, but its colour accuracy isn't great. The measured temperature of 7015K is cool, the Delta E of 5.36 is around one point behind the Lenovo, and the 62.4% sRGB gamut coverage level falls short.
Those latter figures fall behind the Lenovo, but they're not as important – this isn't a machine for serious work, where good accuracy and sRGB figures matter. The Transformer's superior contrast is the key result here, and it means that the Asus's screen will feel punchier and richer when browsing the web, watching videos and playing low-end games.
Asus has fitted surprisingly good speakers to the T200TA. The mid-range is meaty, and the high-end is snappy and well balanced – it's not tinny, and it doesn't overwhelm the rest of the audio. Bass drums aren't too deep and basslines are largely lost, and there's only enough volume to fill a small room – but the audio kit here is good enough considering this machine's design and price.
SEE ALSO: Best Phones Round-up
ASUS Transformer Book T200TA 11.6in. (64GB, intel quad core 1.46GHz, Windows 10 1 bids$99.00 View Item
ASUS Transformer Book T200TA-DB11T-CA Touchscreen Notebook/Tablet (11.6" Dk Bl$219.00 View Item
ASUS Transformer Book 11.6" 2in1 Touchscreen T200TA-B1-BL *No Hard Drive or OS* 26 bids$36.00 View Item
New Asus Transformer Book 2-in-1 11.6" Detachable Tablet 64GB T200TA-C2-BL Wn8.1$269.99 View Item
ASUS Transformer Book T200TA outfit case 11.6" touch Z3775 2G RAM 32G Excellent!$149.95 View Item
Looks good and the price appears low at first glance.
However, the low price has meant a huge sacrifice to the innards. The spec is woeful for everyday use - even casual.
Running Windows on an atom processor is a complete no-no.
I reckon they should have fitted it with at least an i3 processor and 4gb of ram. Yes, the price would go up by a few pounds, but it would be a useable device.
In this spec, one would do well to stay clear.
Really i wonder if the reviwer actually used this keyboard himself. I have written 7 short novellas on mine and find the keyboard to be very consistant and very easy to use, in fact it is one of the best i have used in a long time. Well maybe it is somethignt o do with personal taste but i doubt it, the travel on the keys are excellent, not too much but enough to know when you are touch typing that you have hit the key correctly. Also the keys have never let me down and are the same size as others i have used, Although the keys areflat and dont have a curve to them after a few days of getting used to them they feel more secure when typing than the normal keys on a full keyboard.
Also the touchpad is big, bigger than any other touchpad i have ever used. From the outset gestures work really well two fingers to scroll down or up being the one i use the most, pinch to zoom, brilliant.
The only downfall for mine is that the whole dock is slightly high and could have been a lot thinner i believe. Hopefully in the future there will be replaceable keyboards that are much thinner and pack a battery in them too. I like the hdd access but if they had been clever again they could have created a tray to slide a new hdd into the side of the dock , it is thick enough to do that. In fact the keyboard is as thick if not thicker in places than my old laptop was.so they could probably provide an updated keyboard with the guts of say an android system or a system that has better graphics for games.
Ahhhh so many possibilities they missed out on here, but i am sure they will read my comment and correct these small issues next time around.
More information -
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Home > Indexes > Cisco Systems > WS-C3850-24T-L
Click image to enlarge or see more views.
UPC Code: 882658547744
Provantage Code: CSCO39R
Condition: Factory New
Note: We Are Unable to Sell this Product to Dealers.Provantage is authorized to only sell this product to a final purchaser or licensee that has acquired it for their own use and not for resale, remarketing or distribution.
Networking > Routing and Switching Devices > Switches and Bridges
wS-C3850-24T-L Catalyst 3850 24 Port Data LAN Base
Safety Certifications:
Electromagnetic Emissions Certifications:
Cisco Systems C3850-NM-4-10G= Catalyst 3850 4X100GE Network Module
Mfg Part# C3850-NM-4-10G=
Cisco Systems Cabinet Jumper PC 250-VAC 16A C14-C15 Connector
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Cisco Systems CAT3750X Stack 30CM Power-Cable Spare
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Turn-key Franchise Clothing opportunity is available in the Triangle Area. $170k start-up expenses has already been absorbed and needs the right owner to take it to the next level. The $130,000 price includes $47,000 in inventory at cost and $35,000 in furniture, fixtures and equipment. Seller is...
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New Century Hotel
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TripAdvisor Reviews New Century Hotel Lanzhou
Travel Blogs from Lanzhou
Last day in Lanzou
... various artifacts in the exhibition, all dating back some 2000 years. He points at them, and then carefully formulates the sentence: "Are you interested in the Chinese future?" We eat kabob in a muslem restaurant and slowly walk home through the night. A truck spraying water on the road passes us, playing patriotic music. Neon advertisements blink behind trees. I remark to Sisi that when I prepared for our trip, I imagined that ...
Sightseeing In Shenzhen
... and eating in dimly lit noodle bars, the smell of incense sticks are everywhere and if it wasn't for the humidity and weather some parts reminded me of east London, or even Camden. Graffiti and bizarre 'artwork' could be found on mostly every corner or wall of each building, some quite nice others just weird.
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... If it could be compared to anywhere in England it would probably be like China's answer to York or Chester, but with more noodles. It was a great place for us to chill out for a couple of nights and take it easy, wandering the streets during the day and discovering the amazing local food at night. We didn't spend any time walking the walls or visiting the numerous museums and temples, because we simply couldn't be bothered. We did however spend a lot of time sat in the very hot ...
Day 2 trekking tiger leaping gorge
... fragrant". Getting used to breathing through mouth. Went back for apple pie which was delicious and freshly made. Crispy pastry filled with grated apple. Just needed ice cream (in my dreams. This is china after all). Fran and a few others were playing cards. I watched for a while but was so sleepy so went to bed. Bad pillow again. I dream of my pillow at home. Used my sleeping silk as the blankets did not look very clean. Hopefully good sleep ready for the last day of trekking ...
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Evidence-based antioxidant activity of the essential oil from Fructus A. zerumbet on cultured human umbilical vein endothelial cells' injury induced by ox-LDL
Basic research
The essential oil from Fructus Alpiniae zerumbet (FAZ) is its principal bioactive ingredient, and is widely used in
Cell viability was analyzed with the MTT assay and trypan blue exclusion staining (TBES). Cell injury was assessed by lactate dehydrogenase (LDH) release. Biochemical enzymatic methods were used to evaluate the oxidative stress, including the lipid peroxidation product, malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px).
The redox status of HUVECs was significantly exacerbated after exposure to ox-LDL. EOFAZ protected HUVECs against ox-LDL injury as assessed by the MTT assay, TBES and LDH release. Furthermore, EOFAZ ameliorated the oxidative stress by elevating the activities of SOD, CAT and GSH-Px, and increasing the GSH levels, in addition to attenuating the MDA contents.
The present data provide the first experimental evidence that EOFAZ protects endothelial cells against ox-LDL-induced injury, and indicate that this protection involves ameliorating the redox status.
It is well known that atherosclerosis (AS) is the leading cause of death in most Western countries, and is a crucial pathological factor in the development of cardiovascular diseases, leading to alterations and lesions in the inner walls of blood vessels
The Zingiberaceae is a large diverse family comprising 1,200 species belonging to 49 genera. A prominent member of this family is the
In China,
Chemical and herbal materials
The essential oil was extracted from the fruit of
Cell culture and treatments
Cell cultures were carried out essentially as described previously
For all experiments, cells were used at passages 3 to 6 and seeded at a concentration of 1×105 cells/ml in 96-well plates, 24-well plates, or six-well plates. Treatments were carried out on a confluent monolayer of cells. EOFAZ was freshly prepared as a stock solution in DMSO and diluted with culture medium. DMSO was present at an equal concentration (0.02% final concentration) in all groups except for the PRA group. ox-LDL was used for no more than 2 wk from the date of production (Yiyuan Biotechnology, Guangzhou, China). Cells were preincubated for 30 min with EOFAZ or PRA before being exposed to 100 mg/L ox-LDL for 24 h except for the control group. After treatment, the cells cultured in the 96-well plates were used for the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich) assay, those in the six-well plates were used for trypan blue (Beyotime, Haimen, China) exclusion staining (TBES), and those in the 24-well plates were assayed for the contents of malondialdehyde (MDA), glutathione (GSH) and LDH, and for the anti-oxidative enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). All experiments were performed in triplicate (four or five cultures were used for the experiments), however, the TBES assay was performed only once, but on four cultures.
Cell viability measurements by the MTT assay and TBES
Cell viability was measured by the MTT reduction assay as previously described
The TBES method is another classic cell viability method
LDH activity in the medium
To evaluate cell injury, the LDH released from the cytosol into the culture medium was measured as previously described
Cell lysate preparation
HUVECs were pretreated with EOFAZ or PRA for 30 min and then incubated with 100 mg/L ox-LDL for 24 h in 24-well plates. The cells were lysed with extraction buffer (20 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM glycerophosphate, 1 mM Na3VO4, 1 μg/ml leupeptin and 1 mM PMSF). Cell lysates from each well were collected and used for determination of MDA and GSH contents and anti-oxidative enzymatic activity. Protein concentrations of cell extracts were determined by the BCA assay (Santa Cruz Biotechnologies, Santa Cruz, CA, USA).
Measurement of malondialdehyde (MDA) and glutathione (GSH) concentrations
The total levels of MDA, the lipid peroxidation product in the lysates, were measured by the thiobarbituric acid-reactive substance (TBARS) assay as described previously
Intracellular GSH was measured using the enzymatic recycling assay
Activity assay of anti-oxidative enzymes
SOD activity was measured by the xanthine/xanthine oxidase mediated ferricytochrome c reduction assay
The GSH-Px activity assay was conducted by quantifying the rate of oxidation of the reduced glutathione (GSH) to the oxidized glutathione by H2O2, which is catalyzed by GSH-Px. One unit of GSH-Px was defined as the amount that reduced the level of GSH by 1 μmol/L in 1 min per mg protein. GSH-Px activity was measured in a 1.0 ml cuvette containing 400 μl of 0.25 M potassium phosphate buffer (pH 7.0), 200 μl of sample, 100 μl of 10mM GSH, 100 μl of 2.5 mM NADPH and 100 μl of glutathione reductase (6 U/ml). Hydrogen peroxide (100 μl of 12 mM) was then added, and the change in absorbance was measured at 1min intervals for 5 min at 366 nm
CAT activity was detected by methods of our lab
Statistical analysis
Data were expressed as mean ± S.E.M (standard error of the mean) of at least three independent experiments. The differences in mean values among the experimental groups were measured using two-tailed analysis of variance (ANOVA) followed by Dunnett's test. Differences were considered statistically significant at
EOFAZ attenuates the MTT reduction and blue staining ratio of HUVECs induced by ox-LDL
The MTT assay utilizes the yellow MTT, which is metabolized by the mitochondrial succinate dehydrogenase to yield a purple formazan reaction product. Exposure of endothelial cells to 100 mg/L ox-LDL for 24 h significantly reduced cell viability (Table
Figure 1
Effects of EOFAZ on trypan blue exclusion staining in HUVECs' injury induced by ox-LDL
Effects of EOFAZ on trypan blue exclusion staining in HUVECs' injury induced by ox-LDL. HUVECs were pretreated for 30 min with EOFAZ or PRA, and then exposed to 100 mg/L ox-LDL for 24 h. A cell suspension was mixed with a 0.4% trypan blue dye for 5 min at 25°C, and the unstained (viable) and stained (nonviable) cells were counted in a hemacytometer. The values shown are mean ± S.E.M. of three experiments (four or five cultures per experiment). ##
EOFAZ inhibits LDH release from cells treated with ox-LDL
A 24 h exposure to 100 mg/L ox-LDL resulted in a marked and significant facilitation of LDH release from the cells, compared with the vehicle control group (555.15±59.67 U/L vs. 269.12 ± 97.80 U/L,
Figure 2
Effects of EOFAZ on LDH activity in HUVECs' injury induced by ox-LDL
Effects of EOFAZ on LDH activity in HUVECs' injury induced by ox-LDL. HUVECs were treated as described in Figure
EOFAZ reduces the MDA contents and increases the GSH contents in cells treated with ox-LDL
In the present study, incubation of HUVECs with 100 mg/L ox-LDL for 24 h resulted in a significant increase in the MDA contents, which was significantly attenuated by EOFAZ or PRA pretreatment (Figure
Figure 3
Effects of EOFAZ on MDA contents in HUVECs' injury induced by ox-LDL
Effects of EOFAZ on MDA contents in HUVECs' injury induced by ox-LDL. HUVECs were treated as described in Figure
Figure 4
Effects of EOFAZ on GSH contents in HUVECs' injury induced by ox-LDL
Effects of EOFAZ on GSH contents in HUVECs' injury induced by ox-LDL. HUVECs treatment and cell lysate preparation were performed as described in Figure
EOFAZ upregulates the activities of antioxidant enzymes
The main scavengers responsible for inactivation and termination of free oxygen radicals are SOD, CAT, and the glutathione system. Our results indicated that the activities of the anti-oxidative enzymes were significantly decreased after HUVECs were exposed to 100 mg/L ox-LDL, and that EOFAZ and PRA improved these activities (Figures
Figure 5
Effects of EOFAZ on SOD activity in HUVECs exposed to ox-LDL
Effects of EOFAZ on SOD activity in HUVECs exposed to ox-LDL. HUVECs treatment and cell lysate preparation were performed as described in Figure
Figure 6
Effects of EOFAZ on CAT activity in HUVECs exposed to ox-LDL
Effects of EOFAZ on CAT activity in HUVECs exposed to ox-LDL. HUVECs treatment and cell lysate preparation were performed as described in Figure
Figure 7
Effects of EOFAZ on GSH-Px activity in HUVECs exposed to ox-LDL
Effects of EOFAZ on GSH-Px activity in HUVECs exposed to ox-LDL. HUVECs treatment and cell lysate preparation were performed as described in Figure
To the best of our knowledge, the present study demonstrates for the first time that EOFAZ (the composition was significantly different from previous reports and from the essential oil extract from the fruit of A.
Human umbilical vein endothelial cells (HUVECs) have played a major role as a model system for studying the regulation of endothelial cell function and the role of the endothelium in the response of the blood vessel wall to stretch, shear forces, and the development of atherosclerotic plaques and angiogenesis
The cell injury was evaluated by the MTT assay, TBES, and LDH release, which are widely accepted methods for cell injury evaluation. The MTT assay is a quantitative colorimetric method to determine cell proliferation or injury. It utilizes the yellow MTT, which is metabolized by the mitochondrial succinate dehydrogenase to yield a purple formazan reaction product. The TBES method is widely used to determine the number of viable cells and is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, eosin and propidium, whereas dead cells do not. Lactate dehydrogenase (LDH) is a stable enzyme in the cytosol, present in all cell types, that is rapidly released into the medium upon damage of the plasma membrane; hence it is a biomarker for cell membrane damage. The cell injury induced by incubation with ox-LDL for 24 h was confirmed by the MTT assay (OD570 decrease), trypan blue staining ratio increase, and the LDH activity increase. Pretreatment with EOFAZ or PRA ameliorated the cell injury.
Membranes of bioplasm are very rich in polyunsaturated fatty acids, which are especially sensitive to free radical-induced lipid peroxidation. In oxidative stress, superoxide anion and hydrogen peroxide are formed and cannot be readily scavenged because of the low activities of CAT, SOD and GSH-Px in the endothelial cell
Exposure to 100 mg/L ox-LDL for 24 h, increased the MDA contents, decreased the GSH contents, and inhibited the enzymes' antioxidant activity, indicating that the redox status was exacerbated. In the present study, a 30 min preincubation with EOFAZ significantly elevated the activities of SOD, CAT and GSH-Px, whereas PRA only enhanced the activity of SOD and CAT. Obviously, EOFAZ scavenged hydrogen peroxide and superoxide anion, further decreasing the formation of hydroxyl radicals and attenuating lipid peroxidative damage after the HUVECs were exposed to ox-LDL. However, it is not clear whether EOFAZ induces the expression of the endogenous antioxidant enzymes or whether it has direct protective effects of endogenous antioxidants. PRA, a hydroxymethylglutaryl-CoA reductase inhibitor, is a member of the statins drug class and was used here as a positive control. PRA, which is used for lowering cholesterol and preventing cardiovascular disease, and is widely used to prevent AS, has anti-oxidative effects
Oxidative metabolites are involved in the functional inactivation of endothelial cells by increasing permeability and as potent inducers of endothelial cell death. The level of MDA reflects the extent of cell damage by oxidative stress
Endothelial dysfunction is an early feature of both atherosclerosis and vascular diseases, which may lead to an improvement of prognosis in patients with cardiovascular risk factors preventing the development of atherosclerosis and consequently promoting a reduction in cardiovascular events
In summary, the present research indicates that increase in HUVECs' lipid peroxidation and depletion of endogenous antioxidants support the occurrence of oxidative stress after exposure to ox-LDL. Furthermore, it was accompanied by cell viability decrease, which was confirmed by the MTT assay, trypan blue exclusion, and LDH release. EOFAZ protected the cells against oxidative stress, as evidenced by inhibition of the increased MDA contents and decreased GSH contents, alleviation of CAT, SOD and GSH-Px activities, and finally, enhancement of cell viability. The mechanism of this protection can be attributed to the augmented endogenous antioxidant reserve in HUVECs and/or a direct antioxidant effect. This study shows that EOFAZ from natural products may be an excellent protecting agent against endothelial cell injury in the clinic; the mechanism will be explored in detail and a clinical evaluation conducted in future research.
The present findings provide the first experimental evidence that EOFAZ protects endothelial cells against ox-LDL-induced injury, and indicate that this protection involves amelioration of the redox status.
Ethics approval
This study was approved by the ethical review board of Guiyang Medical University according to local and national guidelines. Care of the patients in this study was in accordance with the Declaration of Helsinki and all relevant laws.
AS: Atherosclerosis; CAT: Catalase; DMSO: Dimethyl sulfoxide; EOFAZ: Essential oil from Fructus Alpiniae Zerumbet; FAZ: Fructus Alpiniae Zerumbet; GSH: Reduced glutathione; GSH-Px: Glutathione peroxidase; HUVECs: Human umbilical vein endothelial cells; LDH: Lactate dehydrogenase; MDA: Malondialdehyde; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OD: Optical density; ox-LDL: Oxidized low-density lipoprotein; PBS: Phosphate-buffered saline; ROS: Reactive oxygen species; PRA: Pravastatin; SOD: Superoxide dismutase; TBES: Trypan blue exclusion staining.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
XS: Supervised the work, provided the grant, designed and performed the study, evaluated the data, prepared and corrected the manuscript, and coordinated the study. LT: Prepared the essential oil from Fructus Alpiniae Zerumbet and performed the biochemical assays. WL: Performed the study and analyzed the data. YZ, HL and YX: Performed the biochemical assays. All authors read and approved the final manuscript.
This research was supported by grants from the National Natural Science Foundation of China (No: 30960531), the Training Program of Guizhou Provincial Excellent Talented Scientists and Technicians (No: 2009–28), the Excellent Scientific and Technological and Educational Talents of Governor's Special Fund in Guizhou Province (No: G2011-2), the Science and Technology Research Key Project of the Ministry of Education (No: 211166), and the Modern Chinese Medicine of Guizhou Province (No:2012–5051).
Role of oxidative modifications in atherosclerosis
Intercellular adhesion melecule ( ICAM )-1 and vascular cell adhesion molecule ( VCAM )-1 at the early stages of atherosclerosis in a rat model
Oxidant stress and endothelial cell dysfunction
Oxidized low-density lipoprotein and atherosclerosis implications in antioxidant therapy
Pharmacological and toxicological evaluation of Alpinia specosa
Composition and seasonal variation of essential oil in Alpinia zerumbet from Okinawa Island
Zingiber zerumbet (L.) Smith: a review of its ethnomedicinal, chemical, and pharmacological uses
Effect of Alpinia zerumbet components on antioxidant and skin diseases-related enzymes
Hypolipidemic effects of different angiocarp parts of Alpinia zerumbet
Alpinia zerumbet potentially elevates high-density lipoprotein cholesterol level in hamsters
Effects of volatile oils from fructus Alpiniae zerumber on the contraction of rabbit thoracic aorta in vitro
GC-MS analysis of chemical constituents of the essential oil from different parts of Alpinia zerumbet (Pers.) Burtt et Smith
Inhibition of ketamine-induced hyperlocomotion in mice by the essential oil of Alpinia zerumbet: possible involvement of an antioxidant effect
Involvement of tyrosine kinase in the hypoxia/reoxygenation-induced gap junctional intercellular communication abnormality in cultured human umbilical vein endothelial cells
Ginkgo biloba extract attenuates ox-LDL-induced oxidative functional damages in endothelial cells
Protective effects of Paeonia lactiflora pall on hydrogen peroxide-induced apoptosis in PC12 cells
Effects of Newcastle disease virus strains AF2240 and V4-UPM on cytolysis and apoptosis of leukemia cell lines
Protective effects of Rheum tanguticum polysaccharide against hydrogen peroxide-induced intestinal epithelial cell injury
Protective effect of tota flavonoids from Spirodela polyrrhiza (L.) Schleid on human umbilical vein endothelial cell damage induced by hydrogen peroxide
γ-Glutamylcysteine inhibits oxidative stress in human endothelial cells
Mitochondrial adaptations to obesity-related oxidant stress
The effect of chronic ethanol feeding on ornithine decarboxylase activity and liver regeneration
Effects of crocetin on antioxidant enzymatic activities in cardiac hypertrophy induced by norepinephrine in rats
Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis
Ozonized low density lipoprotein (ozLDL) inhibits NF-κB and IRAK-1-associated signaling
Effects of extensively oxidized low-density lipoprotein on mitochondrial function and reactive oxygen species in pocine aortic endothelial cells
Targeting endothelial dysfunction in vascular complications associated with diabetes
Free radicals in the physiological control of cell function
Effect of chronic treatment with bark of Terminalia arjuna: a study on the isolated ischemic-reperfused rat heart
Effect of bioactive tannoid principles of Emblica officinalis on ischemia-reperfusion-induced oxidative stress in rat heart
Emblica officinalis causes myocardial adaptation and protects against oxidative stress in ischemic-reperfusion injury
The ability of statins to protect low density lipoprotein from oxidation in hypercholesterolemic patients
Novel role of Zn (II)-curcumin in enhancing cell proliferation and adjusting proinflammatory cytokine-mediated oxidative damage of ethanol-induced acute gastric ulcers
Prodrug approach for increasing cellular glutathione levels
Cardiovascular risk and endothelial dysfunction: the preferential route for atherosclerosis
Does reversal of oxidative stress and inflammation provide vascular protection?
Experimental study on the anti-inflammatory and analgesic effects in vivo of essential oil from frucus Alpiniae zerumbet in mice
Pre-publication history
The pre-publication history for this paper can be accessed here:


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Linux: Secure as You Want It to Be
Yes, Linux will be attacked more often in the days ahead, but far fewer attacks will get through than do on Windows.
My colleague Larry Seltzer thinks that we may be on the verge of an age of Linux worms that might rival the endless trouble that Windows users find themselves in.
First, none of the trio of vulnerabilities in the luppi worm actually have a thing to do with Linux. Yes, these worms target Linux systems, but the holes they use to target aren't Linux holes at all. They're Web service script holes.
Saying that this is a Linux problem is like saying that the gaping Macromedia Flash hole is an XP problem.
So long as you don't run a vulnerable application, your system is as secure as ever.
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Right, to the staircase
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You start to relax- the sight is calming and not hard to enjoy- when you hear footsteps, rapidly making their way up the same staircase that you did. You have but three options…


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I'm having problems with my powerline adapters
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Eyelash curler from the collection. The NARS Eyelash Curler gives straight lashes the perfect curl with its soft contoured pad to fit all eye shapes. With its sleek signature black matte finish and ergonomically designed shape, the NARS Eyelash Curler allows for more control to achieve the ideal look for lashes.
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