text:
The thugs were jailed for life today and handed minimum terms of between 13 and 16 years.
After they were led to the cells Edward Brown QC prosecuting told the court of the attack on the witness who had been granted anonymity and gave evidence from behind a screen.
A clearly shocked Judge Timothy Pontius said: "Whatever measures the court can apply sometimes, I fear, they are not sufficient.
"This witness has the court's sympathy and its thanks for having the courage to come to court and give evidence about what he had seen."
Sunday was just 15 when he was knifed nine times in the back and kicked "like a football" as he begged for mercy in February last year.
His mother died soon after he had been brought to Britain for a better life following the death of his father in Nigeria.
He appears to have been a victim of a turf war between a Somali gang dealing crack and heroin in the area and the T-Block gang from Thamesmead.
The murder was carried out just after 4pm when Sunday was killed after being chased until cornered by a wall on an estate in Plumstead.
Adeniyi Oloyede, 19 - known as Knifer - was sentenced to a minimum of 13 years, Miles Maddy, both of Thamesmead, to at least 15 years, Sikiru Doherty, 20 of Woolwich, and Ifedotun Gbadebo-Araoye, 19 of Charlton, both received a minimum of 16 years.
Oloyede and Maddy were both under community and supervision orders having been convicted of affray when they carried out the murder.
Gbadebo-Araoye was also found guilty of the knifepoint robbery of a chain and was sentenced to a concurrent term of seven years.
During the trial witnesses told how Sunday screamed in terror and begged his killers to spare him.
Judge Pontius described the murder as "savage, merciless and unrelenting."
Friends remember Sunday, who was studying GCSEs at a technology college in Southwark, as a happy football loving Manchester United supporter.
Abu Mansaray, 18, said: "He was struggling, struggling all his life - coming here from Nigeria at primary school age for a better life.
"But he was a happy boy and loved football. when he was around everyone was happy. he was funny, alays cracking jokes."
Despite their relatively young ages, all the defendants have previous convictions, cautions or reprimands for related violence, including possession of a bladed article, common assault, robbery and affray.Reuse content


text:
" I believe in targeting a problem from all angles, not just one. This advanced formula has ingredients that combat all causes of under-eye discoloration, eliminates puffiness and provides moisture – making it unique." - Dr. Dennis Gross
This improved state-of-the-art formulation utilizes the latest technology to revitalize the entire eye area. It diminishes the appearance of fine lines and wrinkles to aid in repairing skin damage. Lift & Lighten Eye Cream also offers benefits of chelators, known to remove harmful impurities such as heavy metals left on the skin from tap water. By removing these heavy metals, the chelator slows down the appearance of fine lines and wrinkles.
Packed with active ingredients, Lift & Lighten Eye Cream contains a potent dose of Vitamin K and Vitamin C to the delicate under-eye skin to effectively diminish under-eye darkness and discoloration. Light-refracting pigments immediately address under-eye discoloration while Peptides and Arbutin promote long-term solutions to pigmentation and dark circles. Vitamin C also improves skin tone and builds collagen, helping to reduce the visible signs of aging.
This advanced revitalizing cream provides essential moisture, protection, and a noticeable skin tightening effect for an immediate firmer, more youthful appearance.
Improved Benefits:
- Helps eye-area appear brighter, firmer, smoother from the first use with chelator, Emblica and Vitamin C and Gamma Amino Butyric Acid (GABA)
- Enhanced lightening with new peptides and chelator
- Two new ingredients to reduce leaky vessels
- Two new ingredients to reduce puffiness
- Brightens eye-area with light diffusers
- Still contains all active ingredients in prior product except Kojic Acid

subset: commoncrawl


text:
We Energies' customers' natural gas use breaks record amid deep freeze
Staving off the Arctic chill, We Energies customers used a record amount of natural gas this week, even after the Milwaukee utility cut off gas service to 40 of its large business customers.
The utility said Wednesday that its customers used a record 1.92 million dekatherms of natural gas between 9 a.m. Monday and 9 a.m. Tuesday.
That was about 7% more than the 1.788 million dekatherms the utility supplied to customers during a cold snap in 2009, said We Energies spokesman Brian Manthey.
To ensure there would be no supply problems, amid predictions of dangerous below-zero wind chill temperatures around the state, We Energies cut off supply to about 40 large businesses starting Monday morning.
Those businesses were without natural gas from We Energies for 48 hours, until 9 a.m. Wednesday, Manthey said. Those customers pay low rates throughout the year in exchange for giving the utility the ability to shut off natural gas service when it needs to.
This was the first time since 1996 that the utility had cut off service to businesses that are on those special rates.
The businesses were cut off as a precaution. Many of those cut off were in northern and western parts of Wisconsin that had wind chill readings below the Milwaukee area.
"We were able to keep any supply situations from happening," he said.
"You didn't know going in how cold will it get, and you want to make sure you plan for some worst-case scenarios and make sure that you're able to meet the demands and not have any disruptions elsewhere on the system," he said.
Based on how much colder than normal the temperatures were Monday, We Energies estimated a typical residential customer would have paid about $7 to keep warm, compared with about $4 on a January day with normal temperatures, according to Manthey.
Alliant Energy Corp., based in Madison, said customers used more natural gas this week than any time since the bitter cold snap of 1996. In addition, the utility interrupted service to about 30 Wisconsin businesses, as well as about 350 in Iowa.
It was the first time since 1999 that Alliant's Wisconsin customers had been interrupted.
Total U.S. natural gas demand hit a record on Monday — and then broke it again on Tuesday, according to Bentek Energy, the oil and gas analytic unit of Platts.
On Wednesday, demand fell 13% but was expected to rank sixth highest since 2005, according to Jack Weixel, Bentek director of energy analysis.
"Bentek expects total demand will continue to decline through the rest of the week as cold temperatures subside across the country," Weixel said.
Gas prices have jumped 20% since Nov. 1 as waves of frigid weather moved across the lower 48 states.
Tuesday was the coldest day of the 21st century in the contiguous United States, based on natural gas-weighted heating-degree days, a measure of energy demand, said Matt Rogers, president of Commodity Weather Group in Bethesda, Md.
Natural gas futures fell to a one-month low in New York on forecasts that the blast of cold air boosting demand across the U.S. will be followed by milder weather.
On Wednesday, natural gas futures slid 1.9% as government weather models showed above-normal temperatures across most of the lower 48 states next week, according to Frontier Weather Inc.
Natural gas for February delivery fell 8.3 cents to $4.216 per million British thermal units on the New York Mercantile Exchange, the lowest settlement price since Dec. 6.
Twitter: twitter.com/plugged_in
Bloomberg News contributed to this report.
Indianapolis developer proposes 274 apartments at former Pabst complex
Peptimed raises $200,000 to aid development of cancer-fighting drugs
Ballpark Commons could get $26 million in city funds, study suggests
'Professional plaintiff' uses credit law to threaten companies, win $230,000 in settlements
PreCheck air travelers aren't always getting what they paid for
Tom Still: Choosing the correct tool to boost start-ups can be a balancing act
Investment Trends: Proliferation of mobile devices should fuel network infrastructure stocks
Whitestone Station bringing stores, restaurants, over 300 apartments
WEDC identifies priorities for economic growth
Steve Jagler: After-hours enrichment sparks Sobic's executive journey to the NFL
Business Wire
Recent national business updates from the Associated Press


text:
Águilas Spain Businesses For Sale
Searched the DealStream database for Águilas Spain Businesses For Sale and found no matches. Expanded the search to nearby areas and found the following matches.
Commercial Center for Rent (Orihuela Costa) #466690 posted Apr 16, 2017
- Orihuela Costa, Valencia, Spain
The building area is 8 175 sq.m. Ground floor - 691 sq.m. The ground floor is 5 015 sq.m. The first floor is 2 414 sq.m. 4 lifts. Toilets. The main stairs and the ladder for evacuation. Unloading area. Parking area for 204 cars, 6 places for disabled people. Plot of land with an area of 12 281 sq.m....
Hotel Four Stars Near Almeria #462220 posted Feb 3, 2017
- Almería, Andalusia, Spain
The hotel has 200 rooms (rooms and apartments). Restaurant, garden with a large swimming pool. The hotel is in very good condition. The hotel is located between Almeria and Roquetas de Mar, in the tourist area, just 200 meters from the beach. Almeria - a city in the southeast of Spain. The city is...
Magnificent Estate 250hec+600hec Protected Forest #461486 posted Jan 23, 2017
- Alicante, Valencia, Spain
Magnificent Country Estate of c.250 hectares, incorporating an Organic Farm, Villa and superb Property Development options, set in a spectacular location. Additional area of c.600 hectares with protected pine forest, mountain range and Private Hunting. This Magnificent Estate encompasses the spectacular...
Did you know that social networking is a great way to buy a business? Sign up now and we'll show you how.


text:
The Black CrowesFri, May 31, 2013 - 8:00 PMVooDoo Lounge KC in Kansas City, MO
We are sorry, the date for this event has already passed. This event took place on 5/31/2013. Click here to view upcoming events for The Black Crowes.
Please continue to check back as additional tickets may become available for purchase at a later time.
Still unsure? Check out our user reviews for:
Have a question? Need help? Call us at 800.477.5285


text:
Power use trends … what's changing?
- Home
- News Hub
- Media releases
- Power use trends … what's changing?
Published: 10 Nov 2015 4:07pm
How does the energy use of home owners and tenants differ?
This is just one of the questions that will be answered when thousands of Queenslanders participate in the State's largest annual research project of household energy use.
The seventh Queensland Household Energy Survey is starting this week with more than 3500 home owners and tenants being asked a range of key questions regarding current and planned energy use.
The survey aims to identify trends in household energy use patterns and energy-saving attitudes and awareness across the State.
Survey questions provide an insight into energy use patterns across Queensland, including:
- Installation and use of air-conditioners
- The surge in large screen, higher energy-intensive televisions
- The growth in solar power.
The annual survey results provide a snapshot of current and potential future energy use that assists with efficient and effective electricity network planning.
The research will be conducted by Colmar Brunton on behalf of Queensland's energy network companies Ergon Energy, Powerlink Queensland and Energex.
Previous surveys revealed the desire by residents to reduce their overall energy use and adopt energy efficiency measures and equipment, yet at the same time showed a surge in the number of higher energy-intensive appliances, especially air-conditioners, which underpin peak electricity demand.
Other insights included a continued interest in domestic solar electricity, increased use of energy efficient air-conditioners rather than electric bar and oil heaters for home heating and the increased adoption of LED and LCD technology.
To take part in this year's survey, go to www.ergonenergy.qldenergysurvey.com.au.
The survey is due to close about mid-November. Survey participants can go in to a draw to win one of 20 $50 EFTPOS cards.
For more information on the survey, or to view last year's results, go to www.ergon.com.au/qhes
Media Contact: Rod Rehbein
Phone: 4153 9813 or 0407 031 072


text:
I am finding more problems with CVF and XP. I am trying to go back to default real*8. This shows up in the compilation options window as "real_size:64". When examining reals in debug mode, they are all single precision except for ones that I have explictly declared as real*8. I've tried deleting all the modules, object files, and executable from the debug directory, but a new compile still ignores the "real_size:64" option. Any thoughts on how to get this to work again? I'm now wondering what other options are being ignored.
More XP problems
For more complete information about compiler optimizations, see our Optimization Notice.


text:
Current versions:
GooReader read books from Google books directly on the desktop in the virtual Bookshelf.
With a virtual Bookshelf GooReader brings Google Books on your computer. The browsing and browsing books and topics from all over the world starts off immediately to the desktop. Clearly as in a classic Bookshelf you have all titles and cover at a glance in front of you.
If you're a fan of Google Books, then you can save you from now long searching and foraging click on the Internet and browser interface. With GooReader you catch a digital Bookshelf right on your desktop. The Internet offering of Google Books, which now includes millions of books from libraries and publishers worldwide, is now clearer and more readable. The books no longer do you need in the browser to read, but can create convenient offline on the entire surface of your desktops.
At a glance the classically designed wooden shelf shows cover, titles, and authors of your Google Books. In the search mask in the headline you will reach by entering by author, title, genre, keyword, or the book ID directly and quickly to your Favorites. Special traffic light labelling in the form of red, green and yellow bookmarks displays friends already in the Bookshelf, which Google Books completely, partially, or not at all exist as a preview. Even if a book not available, but in general information about the book will be shown, about where it can be bought or borrowed. By double clicking on the cover of your choice the Google E-book in a new window opens. Similar like in the Apple's iBook application you come also for GooReader enjoy a dynamic reading through animated page turning. Depending on the requirements, can you zoom you the text also or jump back and forth in the book.
With the paid premium version you can set up your own Bookshelf you by storing the title. But just the experience of the Schmökerns in the virtual GooReader Bookshelf opens a new dimension of E-book reading.
For the current version of GooReader, you need Microsoft.NET Framework 3.5. The advantage of a full-screen mode, where can you put your virtual Bookshelf you on the entire desktop and read books in large-format is the version. The paid Pro version, it is possible to export the books in PDF format. Through a control panel, you can scroll in the book as well as between individual pages and chapters and jump. In contrast to the Web interface of Google Books reading and browsing at GooReaders through the smooth surface has more fun.
New reading and book experience with GooReader
• Read the books in full-screen mode
• animated pages
• Search for book ID, title, author and keywords
• improved readability of the Internet offer from Google Books


text:
John Schwenk, of Sumner Insurance Services in Sedalia, completed insurance training and received continuing education credits awarded by the Missouri Department of Insurance.
The annual regional meeting, hosted by Barton Mutual Insurance, brought together independent agents for instruction in sales, product offerings and industry endeavors.
Barton Mutual is a property and casualty insurer that writes approximately $25 million in premium in homeowner, farm owner, commercial, rental and vacant dwelling lines of insurance throughout Missouri through approximately 250 independent agencies. The company was established in 1894 and continues to operate from Main Street in Liberal.
Release courtesy of Barton Mutual Insurance.


text:
Q Here
I'm going to Dublin on Wednesday and wanted to buy my train tickets in plenty of time. Here's a shot of the queue outside the ticket office in Kent Railway Station, Cork.
Tickets are expensive at 56 Euro for a return ticket to Dublin. A 160 mile journey that takes almost 3 hours!
8 Comments
November 27, 2005
Don't they have one of those automatic machines in Kent station yet?
November 27, 2005
They do actually. 3 in fact. I took a quick look and when it said "for travel today" I joined the queue at the ticket office..
November 28, 2005
Is it not cheaper to go with Ryanair now? I suppose when you factor in the cost of the taxi to the city centre…
November 28, 2005
It probably would be cheaper to fly Ryanair, but it was at short notice and I never thought of it. Looking at Ryanair I see I could have flown for 9.99 up and 14.99 down.
When you add taxes, fees and charges of €45.44 and €41.28 respectively the total cost of the airfare comes to €136.68! The train station is also closer to town and the flight times don't match the schedule we had worked out.
Ryanair 0 – 1 State run monopoly train service
November 28, 2005
Plus you can get the Luas up from Heuston now too!
November 28, 2005
if you buy a faircard, it reduces the price to forty something euro. it actually pays for itself in the saving on the first journey.
September 25, 2006
davidobyrne has posted a comment:
Nice photo – that's me at the front of the queue 🙂 [A friend passed on the link]
November 2, 2006
Phil + Andy has posted a comment:
Jaysus, you can do nothin' eh?
Big Flickr is watching us all…


text:
New Delhi, Jan 9: The 7.8 billion dollars company Tata Motors Ltd today launched a new Tata Sumo 'Grande' priced between Rs 6.6 lakh and Rs 7.49 lakh (ex-showroom Delhi).
The new Tata Sumo is powered by a 2.2 dicor engine and the company expects to make the vehicle available to customers by the first week of next month. The company, that sells about 2,200-2,400 Sumos a month, expects the sales to go up by 30 per cent with this launch.


text: Don't even ask me how Brookers is already 11 months old. I think someone is playing a cruel trick on me and making time go by way too fast. Brooke is definitely our little best buddy and makes everything we do that much more fun because seeing her reactions to life is the best. … Continue Reading


text:
You have no items in your shopping cart.
Marijuana, cannabis, pot and weed are all names that this useful and popular plant and its high producing components are called. It is used throughout the world although most governments have officially listed it as illegal. Cannabis has many uses from a mild and pleasurable drug that can be smoked or eaten, to a source of fiber that is used in clothing and to make specialty papers.
The cannabis plant is very easy to grow and in some countries, it lives up to its name of 'weed' and grows in abundance in the wild. These wild varieties are normally very low in THC, which is that active ingredient that gives the 'high' or 'buzz', and one of the cannabinoids that hold the medical properties that marijuana is also prized for.
This high growth rate and easy of cultivation mean that people that wish to grow there own plants can do so from just a few seeds. The difficulty in detecting seeds has lead to a huge increase in the mail-order business for cannabis seeds. There are literally hundreds of seed selling websites that have sprung up to cater for its popularity. This has led to many that are less than ethical and a few that are just frauds. The illegality of the cannabis seeds shields them to some extent with people scared to make official complaints.
When you are searching for a good marijuana seed supplier then reputation really is everything. It is easy to find sites that have been in business for years and that have a solid user base on the forums. The other thing to be careful of are things that sound 'too good to be true'. High quality well bred seeds cost a lot to produce and are a considerable investment. Bargains generally don't turn out to be good in the long run so take care and do your research before making your purchase.
When you receive your discretely packed seed then you need to think about how you intend to grow them. Remember an adult cannabis plant can be well over a meter tall so you need room for it to grow. As it is illegal to have marijuana and to grow cannabis plants in most parts of the world, planting it in your garden or leaving some potted on your balcony would probably not be a good idea.
It really depends on how many plants you wish to grow at a time and how serious you are in your endeavor to grow your marijuana. If you only want a few plants then pots kept near a window for sunlight or converting a closet into a mini grows room might be what you want to do. Some people decide to use a whole house for the purposes of growing cannabis.
There are hydroponic setups that you can build or buy that are ideal for small setups. They take care of watering fertilizing and lighting. These are the main areas that you need to concentrate on as your plants grow making sure they are warm and well lighted and getting enough food and water.
Good luck with your growing!


text:
Frances (horse)
Frances was a New Zealand thoroughbred racemare foaled in 1942 by Bulandshar (GB) out of Trivet (NZ). She won a number of major races including the 1948 Auckland Cup.
Big race wins
- 1948 Auckland Cup
|The above article was imported from Wikipedia.org on 08/24/2010 and may be a modified version of the Original Wikipedia article. See this article's history page for a list of revisions which may have occurred after import. This article is displayed here courtesy of the Wikimedia Foundation, Wikipedia, and its authors/contributors in compliance with the Wikimedia Terms of Use Agreement. This article was imported and is licensed under the terms of the Creative Commons Attribution/Share-Alike License 3.0 (CC-BY-SA). Clicking on photos, videos, or drawings in this article will display copyright and license information for that non-text media where available.


text:
Start a Trial
Sign in
Historical Events
Family Trees
Become a Member
Become a Member
New Braunfels Herald Zeitung Newspaper Archives
February 20, 1996
- Page 5
First Name
Last Name
With All of the Words
With the Exact Phrase
Go to advanced search
Discover Your History
Issue Date:
Tuesday, February 20, 1996
Pages Available:
Previous Edition:
Sunday, February 18, 1996
Next Edition:
Wednesday, February 21, 1996
New Braunfels Herald Zeitung
Publication Name:
New Braunfels Herald Zeitung
New Braunfels, Texas
Pages Available:
Years Available:
1952 - 2013
Learn More About This Publication
2.17+ Billion Articles and Growing Everyday!
More Than 400 Years of Papers. From 1607 to Today!
Articles Covering 50 U.S.States + 22 Other Countries
Powerful, Time Saving Search Features!
Find Your Ancestors Now
Newspaper Archives
Birth Records
Census Records
Marriage Records
Death Records
Divorce Records
Military Records
View Sample Pages : New Braunfels Herald Zeitung, February 20, 1996
Page 1
Page 2
Page 3
Page 4
Page 6
Page 7
Page 8
Page 9
Page 10
Get Access to These Newspapers
Plus 2.17+ Billion Other Articles
NewspaperARCHIVE.com -
Used by the World's Finest Libraries and Institutions
OCR Text
New Braunfels Herald Zeitung (Newspaper) - February 20, 1996, New Braunfels, Texas Herald-Zeitung EJ Tuesday, February 20,1996 0 5Sports Day "Mantle's a switch hitter because he's amphibious." — Baseball legend Yogi Berra commenting on Mickey Mantle's batting abilities. In the news Unicorns face Tivy in soccer The New Braunfels Unicorns look to keep pace in the District 52 soccer race in a crucial matchup tonight against the Kerrville Tivy Antlers starting at 7:30 p.m. today in Kerrville. The Unicorns are 5-1-1 in district and a game behind Alamo Heights at 7-0-1. In other district action, the Canyon Cougars (4-9-1,1-6-0) take on the Boerne Greyhounds (2-10-1,1-6-1) at 7 p.m. at Cougar Stadium. In girls' soccer, the New Braunfels Lady Unicorns take on the Canyon Cougarettes at 5:30 p.m. at Cougar Stadium. The game time could be moved up to 4:30 p.m. Rangers softball takes 8th The Smithson Valley Rangers girls' softball team finished in eighth place this weekend at the San Antonio Tournament. Fifty teams from across Texas competed in the tournament. The Rangers defeated Fox Tech 24-3,6-2, Alamo Heights 6-2 and Judson 2-1. The losses in the double elimination tournament came to Taft 6-0 and Incarnate Word 7-3. Shelly Sauer pitched for the Rangers who are 3-2 on the season. They next face Antonian today at 4:30 p.m. at Smithson Valley High School. Local racquetball tourney on tap Fob. 23-25 Top caliber players will be competing in the Wurst Racquetball Tournament on Sunday, Feb. 23-25 at the Athletic Club, 943 King Arthur St., in New Braunfels. The tournament proceeds will benefit the Comal County Emergency Children's Shelter in its mission to provide a safe haven for children who are victims of abuse, neglect or abandonment. Sanctioned by the Texas Amateur Racquetball Association, the tournament will attract some of the best racquetball players in the state and nation. TARA members across the U.S. could read about it in "Racquetball Magazine." The event was primarily organized by former National Racquetball champion Brian Stanley, a San Antonio resident and account representative for CTI Long Distance Company. The majority of funds will be coming from outside the community by the participating racquetball players in the tournament. Several community groups are helping sponsor the event, including the New Braunfels Rotary Clubs. Anyone interested in competing should call (210) 625-3459. A small entry fee will include at least two matches of three • games each and a t-shirt. NB Community Night on tap New Braunfels Community Night at the Alamociome is slated for March 2 when the Spurs play the Philadelphia 76ers. Sponsored by the Downtown Rotary Club and Vivroux Sporting Co., the event will benefit the New Braunfels Art League Building Renovations Fund. Tickets and seat selections are available at Vivroux Sporting Co., Walnut at IH-35 near HEB, for $10, $22.50 and $32.50 each Another $8 will take care of bus transportation if desired. For more information, call Vivroux at 606-4080. The New Braunfels Art League is renovat ing two-story building. The NBAL Gallery is at 239 W. San Antonio St. Mackovic to spook at Comal chapter of UT oxes dinner The Comal County chapter of the Univerisity of Texas Ex-Students' Association will celebrate Texas Independence Day on Friday, March 1, at Uilie Marie's Restaurant in Schertz. The special guest speaker for this annual event will be UT head football coach John Mackovic. Social hour is at 6:30 p.m. with dinner at 6 pm Cost for the affair is $20 per person. Al proceeds will go to scholarships for Comal County students to attend UT. Reservations must be made prior to Fab 26 by sending payment to Gail Engler (629* 2109), 416 Keriick Lane, New Braunfels mot T^t.^TWary Walker, (210) 885-4376. Texas Tech basking in first Top IO LUBBOCK, Texas (AP) — Texas Tech is basking in its first Top Ten ranking ever in men's basketball. The Red Raiders (22-1, 11-0 Southwest Conference) moved from 12th to ninth Monday in the weekly poll by The Associated Press of sportswriters and sportscasters. Texas Tech can clinch a share of its first regular-season title since 1985 by beating Southern Methodist (6-16,3-8) tonight. It's the first time a SWC team has moved into the Top Ten in basketball since 1991, when Arkansas finished second. The last time a current member of the SWC reached the Top Ten was 1984 when Houston was fifth in the final poll. "It's a nice compliment to the university, the program and our fans," Texas Tech coach James Dickey said. "This means the same to us as getting to the Top 25. We have to approach it the right way and keep working to get better," he said. Although the Texas Tech women's team won the NCAA championship in 1993, the highest ranking for the men before this season was 16th in 1985 and 1976. Tech has a chance to become the first team to go through the conference unbeaten since Houston's "Phi Slama Jama" team of 1983 that starred Akeem Olajuwon and Clyde Drexler. The main barrier to doing that is Texas, whom the Raiders barely beat in Lubbock. Tech has to play the Longhorns in Austin on Saturday. "We've been working hard to get to this level," forward Jason Sasser said Monday. "But if we don't take care of our business, it won't mean much. We always thought we had the talent and ability to put ourselves in this position.'The Raiders' only loss was to Eastern Michigan in the Sun Carnival Classic in El Paso on Dec. 27. Eastern Michigan was No. 23 last week, but lost and fell out of the rankings. Unicorns ready for Buffaloes By THOMAS GODLEY Sports Editor HeraW-Zeitung photo by MICHAEL DARNALL Senior forward Ian Owens leads the Unicorns into battle tonight against Fox Tech in a bi-district playoff game at Littleton Gym at Blossom Athletic Center in San Antonio. It will not be entirely unfamiliar terrain for the New Braunfels Unicorns tonight when they step onto the court against a formidable playoff opponent in the first-round of the Class 4A state playoffs. The Unicorns (14-16) take on the Fox Tech Buffaloes (24-6) led by Robert Bell, a 6-8 junior who is averaging 23 points per game. The tip off is set for 7 p.m. today at Blossom Athletic Center in San Antonio. Last year at this time, the Goliath was Boerne and guard Erie Halgenseth, who led the area with 34 ppg. The Unicorns stayed close but eventually succumbed to the Greyhounds in the second half. However, if anyone knows what it takes to knock out a big-time foe in round one, it is coach Cliff Wilkins and assistant coach Victor Sierra. In 1985, on their way to the state tournament, Wilkins and company upset the No. I team in San Antonio when they beat Southwest High School and 6-8 Johnny Robertson. Sierra, Ti lo Schmidt, David Caddel and Damon Millet and Leon Sneed each scored in double figures to shock the Dragons 71-67. Wilkins hopes he can get that same kind of balanced scoring from his 1995 line-up of Ian Owens, Tony'Bradford, J.P. Wilkins, Mark Phair and Aaron Moore. Ray Chapa and C.J. Burden will bolster the attack off the bench. "With a week's practice we feel like we can prepare well for any team," Wilkins said. "We have our work cut out against a very talented Fox Tech team. There's no question we'll have to play an almost perfect game." The Unicorns are holding opponents to 54 ppg, while the Buffaloes are averaging 72 ppg. Wilkins said the Unicorns will attack the Buf-faloes'fu ll-court press and traps, with good passing. On defense they must try to slow down the Buffaloes' up-tempo offense. NBHS softball team captures local tourney From staff reports The New Braunfels Unicorns girls' softball team captured its first-ever tournament championship late Saturday in the NBHS/CHS Softball Tournament. The Unicorns (3-0) topped the Canyon Cougarettes in a 12-6 slugfest in the final game at CHS Softball Field. Coach Cindy Manley said the first tournament victory was a boost to the softball program which is in its third year of existence. "We're excited about the win," Manley said. "I was real pleased with our batting. When the other team gets 15 hits and you still come out ahead, that has to make you feel good." The Unicorns pelted the Cougarettes with 17 hits. After falling behind 3-0, the Cougarettes rallied to score two runs in the second inning and tied the game at 3-3. With two outs in the third inning, the Unicorns broke open the game with five runs. Rebekah Espinosa led the way with an RBI single, which was followed by Emily Wotipka's one-run shot. Tammy Braune and Jennifer Ybarra singled in two more runs as the entire Unicom lineup reached the plate. Roxanne Blue capped the inning with a two-run single. The Cougarettes scored three runs in the fourth inning to pull to within 8-6. The Unicorns answered with three tuns in the sixth and one in the seventh to close out the scoring. New Braunfels pitcher Braune Herald-Zeitung photo by MICHAEL DARNALL Tammy Braune helped guide the Unicorns to the NBHS/CHS Softball Tournament title. struck out three batters and earned the win at the mound. Canyon pitcher Haiborth walked six batters. Both teams had three errors in the game. Marcy Allen led the Cougarettes offense, recording three hits in four plate appearances, including a double. Canyon reached the championship game by defeating Kerrville Tivy in the semifinals. It was the Unicorns third straight victory after beating Seguin 10-9 and Luling 18-2 earlier in the week. 'Canyon is lough, but we matched them," Manley said. "We're learning something with every game." New Braunfels next takes on La Vernia in a double header on Tuesday before traveling lo play in the Austin Parks and Recreation Tournament which features more than 32 teams from Central Texas. TODAY'S CROSSWORD PUZZLE ACROSS 1 Needs 6 Two of a kind 10 Erupt 14 Unaccompanied 15 Teen woe 16 Stack 17 Store events 18 Baby buggy Brit 19 "... a bushel — peck- 20 Retreat 22 Pearl maker 24 Gangster s girlfriend 25 Gossip 26 Trade 30 Writer Dmesen 32 Bread spread 33 Tackle (the quarterback) 35 Woodwinds 40 More verdant 42 Base 44 Large-eyed monkey 45 Key — pie 47 Stand 48 Tiny particle 50 Felt 52 Leave 56 Org 58 "— Twist* 59 Kind of dome 64 Singer Turner 65 Arab ruler 67 Novelist Jong 68 Days before holidays 69 "Song — Blue" 70 Fury 71 Take a break 72 Writer James — 73 Tears apart DOWN I Cowboy star LaRue Jai — Frigid Leg part Type of bagel Of pontiffs Plastic 8 One — million 9 Shark s passenger 10 Twitch II Chili bean 12 Church official 13 Has on 21 Inferior 23 Whitehorse's territory 26 Colton pod 27 Sheltered. on a ship 28 Paper measure 29 Soy product PREVIOUS PUZZLE SOLVED md nam bhh bbb hbemhb HHamtsBBB MHHHB0 HMBMHBB EDC10 UHM MUMM B DBB MQHBB ll MUJ 14 BM MBD0UUU MWW BH0BOB WB MU 14 Ii] Ll OWW MSLB BfflOBDQ BOHMHBM MB HE MU 14 BM 14MB HBM SHMHB U14UM BBQD QUIZ] Id 14UM □BUDHU BH1411L4UM BQBBHB BB0UBMB BB BHB UMM BUU 2 9 96 O 1996. United Feature Syndical* 31 Nonfat milk 34 Singer Guthrie 36 Nee 37 Kimono sashes 38 Someone —: not me 39 Plant-to-be 41 In a bad mood 43 Artist s plaster 46 Suppose 49 Singer Brewer 51 Make beloved 52 Election Day participant 53 In existence 54 Movies 55 Sea captain s command 57 Suit material 60 Shore bird 61 Poster 62 Frosted 63 Jam ingredients? 66 Coffee cup Cowboys' Sanders puts baseball on hold By DENNE H. FREEMAN AP Sports Writer IRVING, Texas (AP) — Deion Sanders and Jerry Jones may have to film another pizza commercial. The ad featuring Sanders joking about playing "both" baseball and football is no longer true. Sanders had a news conference scheduled today to announce he's putting his baseball career on told so he can concentrate on being the NFL's only two-way player, a source told The Associated Press. Jones, the owner of the Dallas Cowboys, and coach Barry Switzer were to join Sanders at his new club, "Prime Time 21," for the announcement. The stow was to begin at noon. Switzer and Jones have been trying for months to convince Sanders to give up baseball — at least for one season — to let his body recover from the wear and tear of being a full-time two-sport athlete. They abo want him to concentrate polishing his skills as a wide receiver. Cowboys quarterback Troy Aik-man has said he thinks Sanders could be a star pass catcher if he worked on it. And, Sanders has said he'd like to see what a spring and summer would be like with his wife and two children. They can spend their time together lounging around their new $2 million home in north Dallas. Sanders can easily walk away from baseball because he's an outfielder without a team The San Francisco Giants allowed him lo become a tree agent by not offering him arbitration. He made $2.5 million last year with the Giants and Cincinnati Reds. Sanders makes even more — a $13 million signing tonus on a $35 million contract — as a cornerback for the Cowboys.But his Dallas debut was delayed last fall because of surgery to repair an ankle injury sustained while playing baseball. Next season, the Cowboys will need Sanders to be healthy and available for every game because they are thin at cornerback and could use another wide receiver. Dallas' defensive backfield may be in trouble because Super Bowl MVP Larry ______________________ Brown is a free agent and unlikely to be re-signed, OTI J PFD*? Kevin Smith is trying to recover from a tom Achilles O I wlvli I—Ly ■ tendon and Clayton Holmes. Call for Answers • Toudv ton* or notary Phone# 1 -900-464-3535 ext. code 540• wa par mnule Sports Day ■ To talk with Sports Editor Thomas Godley about Sports Day, call 625-9144. ext. 24. ;
About Us
Contact Us
Free Newspaper Archives
© Copyright 2017 NewspaperARCHIVE.com. All rights reserved.


text:
An easily understood though exhaustive study of the spiritual life that is full of learning and that quotes copiously from the Bible; Saints; Fathers and Doctors of the Church and classic spiritual writers. Embued with holiness; the author makes his work come alive with its simplicity and understanding. A veritable encyclopedia of what the Saints and other mystical souls have told us. Remarkably easy to read and to understand.
To view this DRM protected ebook on your desktop or laptop you will need to have Adobe Digital Editions installed. It is a free software. We also strongly recommend that you sign up for an AdobeID at the Adobe website. For more details please see FAQ 1&2. To view this ebook on an iPhone, iPad or Android mobile device you will need the Adobe Digital Editions app, or BlueFire Reader or Txtr app. These are free, too. For more details see this article.
Preview Ebook
If you find anything wrong with this product listing, perhaps the description is wrong, the author is incorrect, or it is listed in the wrong category, then please contact us. We will promptly address your feedback.


text:
Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
this person was in a car accident two years ago, in which i was found at fault. they have had back surgery in excess of the ins. policy limits. if this person wins, what can they get? i have no money, i make 10.00 an hour. i have a car which if this happens i plan to sell to a family member. can they garnish my wages or something? i really dont know what they could get from me i have NO ASSETS.
You appear to be a "Judgment Proof Defendant", because you have no assets that can be attached. The Attorney for the victim has likely completed due diligence and determined that you have no assets to attach or lien. However, this does not prohibit an attachment or garnishment of your future wages.
As the operator of the vehicle you are liable for any damages or injury that you cause to persons or property during the course of operating a motor vehicle. You could be held liable for any amount awarded over the policy limits if the matter is litigated. The victim's Attorney could seek contribution from you, or in the alternative attach/garnish future wages.
Good Luck.
The response given is not intended to create, nor does it create an ongoing duty to respond to questions. The response does not form an attorney-client relationship, nor is it intended to be anything other than the educated opinion of the author. It should not be relied upon as legal advice. The response given is based upon the limited facts provided by the person asking the question. To the extent additional or different facts exist, the response might possibly change. Response is for informational purposes only. Time is of the essence. Do not delay. See a lawyer immediately.Ask a similar question
As you had insurance and are obviously being represented by the insurance company's defense attorney appointed to represent you, it would not be appropriate for attorneys here on Avvo.com to give you specific legal advice as to your situation.
The attorney being paid for you by the insurance company is your attorney and he is obligated to answer your questions. You should direct your questions and concerns to your attorney. He is being paid good money to provide you with sound legal advice.
Legal Disclaimer:
Mr. Lundeen is licensed to practice law in Florida and Vermont. The response herein is not legal advice and does not create an attorney/client relationship. The response is in the form of legal education and is intended to provide general information about the matter within the question. Oftentimes the question does not include significant and important facts and timelines that, if known, could significantly change the reply and make it unsuitable. Mr. Lundeen strongly advises the questioner to confer with an attorney in your state in order to insure proper advice is received.Ask a similar question
Post a free question on our public forum.Ask a Question
- or -
Search for lawyers by reviews and ratings.Find a Lawyer


text:
ED Magedson – Founder
Ripoff Report
Jake SteingartInternet USA
Jake Steingart Jim, Justin, Just in case thief, con artist, inappropriate, unlawful Boca raton Internet
Does your business have a bad reputation?
Fix it the right way.
Corporate Advocacy Program™
This man will promise you ridiculous money to model for hs website doing seemingly innocent things. he nver has you sign a model contract or a property release form, but will refuse to take down your videos and images once you request that he does. I am now married and have a baby and he still won't take down the videos I did for him and says I will have to take him to court. He not only sells your videos, he also posts short clips of your videos all over youtube to drive traffic to his adult website. He never even asks you for your ID and that is a federal offense!
This report was posted on Ripoff Report on 04/06/2014 07:01 PM and is a permanent record located here: http://www.ripoffreport.com/reports/jake-steingart/internet/jake-steingart-jim-justin-just-in-case-thief-con-artist-inappropriate-unlawful-boca-1136808. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
Ripoff Report has an exclusive license to this report. It may not be copied without the written permission of Ripoff Report. READ: Foreign websites steal our content
Click Here to read other Ripoff Reports on Jake Steingart
If you would like to see more Rip-off Reports on this company/individual, search here:Search Tips
In order to assure the best results in your search:
Advertisers above have met our
strict standards for business conduct.


text:
HIS Radeon HD 6970 IceQ Turbo 2GB Video Card Review
Batman Arkham Asylum
Version and / or Patch Used: 1.1
Timedemo or Level Used: Built-in Test
Developer Homepage: http://www.batmanarkhamasylum.com/
Product Homepage: http://www.batmanarkhamasylum.com/
Batman: Arkham Asylum exposes players to a unique, dark and atmospheric adventure that takes them to the depths of Arkham Asylum - Gotham's psychiatric hospital for the criminally insane. Gamers will move in the shadows, instigate fear amongst their enemies and confront The Joker and Gotham City's most notorious villains who have taken over the asylum.
Using a wide range of Batman's gadgets and abilities, players will become the invisible predator and attempt to foil The Joker's demented scheme.
Batman: Arkham Asylum features an original story penned exclusively for the game by famous Batman author and five-time Emmy award winner Paul Dini, whose credits include Lost season one and Batman: The Animated Series.
Firing up Batman AA, we can see some strong performance across the board which comes as no surprise. The overclock sees a nice boost in performance at the highest resolution. Below 2560 x 1600 we seem to hit a CPU limitation, so the extra MHz on the card doesn't really help.
PRICING: You can find products similar to this one for sale below.
United States: Find other tech and computer products like this over at Amazon's website.
United Kingdom: Find other tech and computer products like this over at Amazon UK's website.
Canada: Find other tech and computer products like this over at Amazon Canada's website.
- Page 1 [Introduction and Package]
- Page 2 [Card & Specification]
- Page 3 [Test System Setup and 3DMark 11]
- Page 4 [3DMark Vantage]
- Page 5 [Unigine Heaven Benchmark]
- Page 6 [Benchmarks - Resident Evil 5]
- Page 7 [Benchmarks - Tom Clancy's H.A.W.X. 2]
- Page 8 [Benchmarks - Mafia II]
- Page 9 [Benchmarks - Lost Planet 2]
- Page 10 [Benchmarks - Aliens vs. Predator]
- Page 11 [Benchmarks - Street Fighter IV]
- Page 12 [Benchmarks - Far Cry 2]
- Page 13 [Benchmarks - Batman Arkham Asylum]
- Page 14 [Benchmarks - High Quality AA and AF]
- Page 15 [Temperature Test]
- Page 16 [Sound Test]
- Page 17 [Power Consumption Tests]
- Page 18 [Total Performance Rating (TPR)]
- Page 19 [Total Value Rating (TVR)]
- Page 20 [Final Thoughts]
- We at TweakTown openly invite the companies who provide us with review samples / who are mentioned or discussed to express their opinion of our content. If any company representative wishes to respond, we will publish the response here.
Related Tags
Latest News Posts
- MSI GeForce GTX 1080 Ti Lightning Z in detail
- Intel preparing AIO liquid cooler for enthusisats
- Intel Core i9-7980XE: 18C/36T chip costs $2000
- Scale 'The Great Wall' to take home a Blu-ray on us!
- Western Digital reveals two 64-layer 3D NAND SSDs
Forum Activity
- Intel Introduces X299, Kabylake-X, and Skylake-X
- Lian Li PC-Q25B - distance between stand and edge of case
- LaCie Bolt3 2TB Thunderbolt 3 Review
- Asrock X99 Extreme11 reports only 48GB instead of 64 GB
- H270m Pro4 Can't boot with my SSD, stuck on splash
Press Releases
- HyperX expand DDR4 memory with higher density kits up to 4,000MHz
- Western Digital to deliver world's first client solid state drives with 64-layer 3D NAND technology
- Qualcomm fuels IoT growth by currently delivering more than 1 million chips a day into a wide range of connected applications
- Team Group announces theme for COMPUTEX 2017 showcase: go beyond the limit and reach for the top
- SAPPHIRE announces PULSE Radeon RX 560 graphics card


text:
The Wind And The Sea
The woman stood at the brink of the precipice, fair and slender under a waxy moon.
Beneath the cliff she balanced precariously upon the edge of, the sea frolicked, waves rippling towards the base as if rushing to look up at the woman in her final moments.
A breeze blew across the surface and caressed her pale smooth skin, teasing through her long golden curls and making the long skirt of her simple white chiffon dress flutter behind her. Her eyes were closed, revealing ids of a pale lavender colour; her rosy lips were slightly parted, as if she made a last wish on the stars that shone above her like tears waiting to fall upon her death.
In her delicate fingers she held a single red rose. The thorns did not prick her palms as she held it out like an offering. The wind claimed several of its petals, and they spiralled elegantly to the sea where they rested for a moment before being swallowed, tracing the path she was soon to follow.
She enjoyed the wind's gentle touch for a few more moments; the last happiness she would feel. She listened to its voice that called plaintively from the still night.
and the wind sang to me
of an ocean of sorrow across which it howled its despair
of the souls who begged to remain undisturbed in their slumber
of a thousand years and a thousand tears
Her full lips turned up in her final smile, and she spread her arms wide, surrendering the rose. She leaned, her bare feet keeping their grip on the grassy cliff's edge until the last moment as she tilted. She fell.
at the edge of the world where the sky meets the sea
you will find the answers that you seek


text:
First glimpse of the Bibliotheque Rouge editions of JSK's Encyclopaedia Goetica series..
More information on Bibliotheque Rouge can be found here:
First glimpse of the Bibliotheque Rouge editions of JSK's Encyclopaedia Goetica series..
More information on Bibliotheque Rouge can be found here:
Scarlet Imprint are featured in episode 93 of The Occult of Personality podcast.
...Peter and Alkistis describe their magickal paths and how they arrived at the vocation of publishing fine books. They describe Scarlet Imprint and its purpose of producing magickal texts that are original, unique, and beautiful talismans. They discuss their dedication to providing quality texts to other practitioners and breaking boundaries.
This was an in depth discussion we were delighted to engage in.
We hope others find it equally stimulating.
The podcast can be listened to here: http://www.occultofpersonality.net/scarlet-imprint/
The topics covered range from Babalon to Thelema to ritual and with a particular emphasis on the radical ideas proposed in XVI.
We fully support the work of Greg in producing these podcasts which are a real asset to the global occult community. Do show your support by considering donating or subscribing to the podcast.
Raw Power: Witchcraft, Babalon and Female Sexuality can be read and downloaded in pdf form here:http://www.scarletimprint.com/pdf/Raw_Power.pdf
December 4 2010
14:00- 00:00
St James Wine Vaults, St James Square, Bath, BA1 2TW
Jake Stratton-Kent and Misha Newitt are hosting a day of goetia and a night of revelry and hijinks to celebrate the launch of Geosophia : The Argo of Magic. This revolutionary two volume work, published by Scarlet Imprint, traces a continuous history of Western magic from the Greeks to the Grimoires.
The necromantic settting of the St James Wine Vaults in Bath plays host to a full programme of talks, an exhibition of art and an evening of socialising, dancing and imbibing.
A contribution of five pounds for the lectures, exhibition and evening event is requested.
Jake will be performing the opening ceremony, 'The Opening of the Pyramid' adapted from Liber Pyramidos and giving a talk on Chthonic religion and Goetia. An energetic and engaging speaker, Jake has both the intellectual firepower and the magical fire to communicate his daring and meticulously researched thesis which challenges the received wisdom of the Western Magical Tradition.
Geosophia is available for subscriber pre-order and will be ready to ship in late November.
Johnny Jakobsson will hold a lecture named: Nebiros et Ars Necromantica. This will be his second lecture in the UK following the release of his essay Le Grand Grimoire – Pacta Conventa Dæmoniorum in Diabolical (2009). Those who saw his performance at the Black Mass at the Horse Hospital last year will be excited to see him hold forth again.
This time he will take us on a journey from the ancient Greek tradition of the Nekuomanteion and into the forbidden art of medieval European necromancy. Through his own empirical studies of the European grimoire tradition, he will for the first time elucidate Nebiros' chief position in the hierarchy of the cemetery and furthermore shed some light on the notorious tradition of the Hand of Glory.
Johnny will also exhibit magical mirrors in the guise of demonic portraits. In conjunction with the physical presence of Geosophia the paintings are intended to provide means of presence for the spirits of the art.
Mr. Jakobsson's collaborator Michael Idehall will also display paintings inspired by his necromantic practises and the alchemical aspects of death in particular. He has previously touched on the subject in his article The Tower of Babel published in XVI (2010).
Peter Grey will be presenting a lecture entitled Corrupted Spirits and Infernal Keys on his work with Goetia and the forthcoming Scarlet Imprint title Abominations, destroying many of the misconceptions that surround the so-called Lemegeton or Goetia of Solomon the King.
The evening will conclude with a troop of dancers, music and merriment.
Please rsvp via facebook here
Or if you are fortunate enough not to be sharing your intimate details and esoteric proclivities with every intelligence spook, potential employer and marketing agency send us a discrete email to scarletimprint at gmail dot com


text:
|Home | About | Journals | Submit | Contact Us | Français
Upregulation of the matrix metalloproteinase MMP-9 plays a central role in tumor progression and metastasis by stimulating cell migration, tumor invasion and angiogenesis. To gain insights into MMP-9 expression, we investigated its epigenetic control in a reversible model of cancer that is initiated by infection with intracellular Theileria parasites. Gene induction by parasite infection was associated with tri-methylation of histone H3K4 (H3K4me3) at the MMP-9 promoter. Notably, we found that the H3K4 methyltransferase SMYD3 was the only histone methyltransferase upregulated upon infection. SMYD3 is overexpressed in many types of cancer cells, but its contributions to malignant pathophysiology are unclear. We found that overexpression of SMYD3 was sufficient to induce MMP-9 expression in transformed leukocytes and fibrosarcoma cells, and that pro-inflammatory phorbol esters further enhanced this effect. Further, SMYD3 was sufficient to increase cell migration associated with MMP-9 expression. In contrast, RNAi-mediated knockdown of SMYD3 decreased H3K4me3 modification of the MMP-9 promoter, reduced MMP-9 expression and reduced tumor cell proliferation. Furthermore, SMYD3 knockdown also reduced cellular invasion in a zebrafish xenograft model of cancer. Together, our results define SMYD3 as an important new regulator of MMP-9 transcription, and they provide a molecular link between SMYD3 overexpression and metastatic cancer progression.
Cancer progression is a multi-step process resulting from altered gene expression through genetic and epigenetic mechanisms (1, 2). As epigenetic events might be reversed using targeted therapies, there is intensive effort to understand the interplay between these mechanisms in the transcriptional control of key regulatory molecules and the altered epigenetic landscape of cancer cells (2). To investigate epigenetic events in cancer progression, we studied a transformation model offering several unique experimental features. Infection by intracellular Theileria parasites causes a lymphoproliferative disease in cows with clinical features similar to some human leukemia. T. annulata infects mainly B cells and macrophages, whereas T. parva infects B and T lymphocytes (3, 4). Theileria-infected cells are transformed and immortalized; displaying uncontrolled proliferation independent of exogenous growth factors in vitro and increased ability to migrate and form metastases in immunodeficient mice (5, 6, 7). Interestingly, Theileria-dependent transformation is reversible; animals treated with the theilericidal drug Buparvaquone are cured in most cases (3, 8, 9). When Theileria-infected cells are treated in vitro with Buparvaquone, the intracellular parasite disappears from the host leukocyte, which either enters apoptosis or stops proliferating, regaining growth factor-dependence (8). Studies showed that Theileria alters host signal transduction pathways, but there is no evidence of permanent host genome changes (4). Theileria infection activates the JNK pathway and the AP-1 transcription factor (10, 11), which are critical for both the survival and invasiveness of transformed B lymphocytes (5). Theileria also induces the IKK pathway leading to NF-κB activation (12) and the TGF-β pathway implicated in virulence (6). This system offers a unique model to address reversible, epigenetic changes involved in tumorigenesis.
Matrix Metalloproteinase (MMP) proteins may function as mediators of metastasis of Theileria-infected cells (13-15). The large family of MMP proteases play critical functions in development and cancer progression (16). In particular, MMP-9 plays an important role in tumorigenesis and metastasis formation by regulating tumor growth, angiogenesis, cell migration and invasion (16). MMP-9 is a collagenase that degrades Extracellular Matrix (ECM) components including collagens and laminin. Its role in cancer progression is related to ECM degradation and growth factor release (17). The MMP-9 gene is mainly regulated at the transcriptional level by trans-activators binding to the promoter, including AP-1 and NF-κB (18, 19). Recent studies provided insights into how MMP-9 expression is regulated in different cancer types and the role of chromatin modifications (20). MMP-9 transcription involves a stepwise, coordinated recruitment of activators, chromatin modifiers, co-activators and general transcription factors to its promoter (21, 22).
Although MMP-9 was linked to the invasive phenotype of Theileria-transformed cells (13-15), the involvement of epigenetic regulatory mechanisms (and their reversibility) has never been explored in Theileria-infected leukocytes. We studied MMP-9 expression in Theileria-infected cells as a model for epigenetic regulation in leukocyte transformation. The invasive B-cell lymphosarcoma cell line TBL3 was generated by in vitro infection, with T. annulata, of immortalized BL3, a bovine B sarcoma cell line (23). TBL3 cells have a higher invasive capacity than BL3 cells and elevated MMP-9 expression (15). Here, we show that Theileria infection induces the reversible expression of the histone methyltransferase SMYD3 (SET and MYND-domain containing 3). SMYD3 encodes a histone H3K4 di- and tri-methyltransferase involved in tumor proliferation in colorectal, hepatocellular and breast carcinomas (24, 25). SMYD3 binds specific DNA sequences, 5′-CCCTCC-3′ or 5′-GGAGGG-3′, in the promoter region of target genes, leading to transcriptional activation (24). However, the role of SMYD3 in cancer progression is unclear and few direct target genes are known. We show that SMYD3 protein binds to the MMP-9 promoter and regulates expression in Theileria-infected cells and human tumor models. Moreover, SMYD3 knockdown led to decreased H3K4me3 marks on the MMP-9 promoter and reduced MMP-9. Interestingly, SMYD3 levels impact the transformed phenotypes and tumor invasiveness. Thus, our work identifies SMYD3 as a novel regulator of the MMP-9 metastatic gene affecting tumor migration and invasion in vitro and in vivo, thereby highlighting its potential as a therapeutic target to treat metastatic disease.
Parental BL3 and infected TBL3 cells, previously described (23), were cultured at 37°C in RPMI1640 (Gibco) supplemented with 2mM L-Glutamine, 10% Fetal Calf Serum (FCS), 100 units/ml penicillin/streptomycin, 10mM Hepes and 50mM 2-mercaptoethanol. HT1080-2.2 cells (with an integrated 2.2 Kb MMP9 promoter-Luciferase) obtained from Dr. C Yan (Albany Medical College, USA) (26) were cultured at 37°C in DMEM with Glutamax and high glucose, supplemented with antibiotics and 10% FCS. Cells were treated with Buparvaquone (BW720c) (Calbiochem) at 50 ng/ml for 64 hours or with Phorbol-12 Myristate 13-Acetate 9 (PMA) (Sigma p1585) 50 ng/ml for 8 hours. Composition of all buffers are detailed in Supplementary material.
1×104 cells were plated in 96-well plates in triplicate and Buparvaquone was added at different concentrations. After 48 hours, cell viability was measured using the Cell proliferation Kit II–XTT (Roche) and the GloMax®-Multi Detection System (Promega).
Bovine TBL3 and BL3 cells (5×104) were incubated with/without Buparvaquone, resuspended in 1% FCS and seeded onto Matrigel™ (BD Biosciences) (70 μl) in Boyden chamber, BD Falcon™ Cell Culture Inserts (8 μm pores, BD Biosciences). Lower chambers contained 20% FCS medium as a chemoattractant. After 24 hours at 37°C, migrated cells were stained with crystal violet and counted under the microscope (40x objective, at least 10 fields per filter). To test migration of human HEK293 or HT1080 cells, cells were trypsinized and placed in the upper chamber (1×104 cells) coated with 10 μg/ml collagen I in media containing 0.3% FCS and 20% FCS in the lower chamber. After 16 hrs, cells were fixed with 4% formaldhehyde and migrated cells were stained and counted. All experiments were performed in triplicate. Migration index is the number of migrated cells per filter (Control set to 1).
Cells were suspended in 0.35% agarose containing 10% FCS and plated above 0.5% agarose at 1×104 cells per 6-well plate. After 10-14 days, colonies were stained with crystal violet and scored. Experiments were performed in triplicate.
Total RNA was extracted using an RNeasy Plus Mini Kit® (Qiagen). cDNA synthesis was performed with RT Superscript III (Invitrogen) and semiquantitative PCR analysis with goTaq polymerase (Promega). Quantitative PCR amplification was performed in the ABI7500 machine (Applied Biosystems) using Sybr Green (Applied Biosystems). See Table S1 for primer sequences. Relative quantities of mRNA were calculated using the deltaCt method.
Proteins were extracted with Laemmli lysis buffer, resolved by SDS-PAGE, and transferred to nitrocellulose membranes, before incubation overnight at 4°C with antibodies against MMP-9, SMYD3 or β-Actin, (see Supplementary data) followed by secondary antibodies. Membranes were developed using SuperSignal® (Thermo Scientific).
Supernatants from cells grown in serum-free media were mixed with lysis buffer and loaded into SDS-Polyacrylamide gels, co-polymerized with 0.1% gelatin. Gels were washed twice (30 minutes in renaturing buffer (2.5% Triton X-100) and incubated at 37°C, 18 hours in a Gelatinase solution. Gels were stained with 0.5% Coomasie Blue and de-stained with Methanol:Acetic Acid (50:10).
HT1080 and HEK293T (human embryonic kidney transformed with the AgT) cells were transfected with Lipofectamine2000 (Invitrogen). The pGL3-Nkx2.8–WT-SBE and pcDNA-SMYD3 vectors were obtained from Dr. Y Furukawa (Tokyo University, Japan) (24). The MMP9 promoter-Luciferase construct was obtained from Dr. D Boyd (MD Anderson Cancer Center, USA).
Dual luciferase assays (Promega) were performed using the GloMax®-Multi Detection System (Promega) to measure Firefly and Renilla Luciferase activity. The pGL4 hRluc/TK Vector was used for luciferase assay normalization.
For short-term depletion experiments, cells were transfected with siRNA oligonucleotides (60nM) Table S1. The shRNA vector targeting human/bovine SMYD3 was constructed by cloning double-stranded oligonucleotides into the pSuper-vector (Oligoengine), generating siRNA directed against the sequence AGCCTGATTGAAGATTTGA. shCtrl plasmid is an empty pSuper-vector.
Chromatin was extracted from 5-20 ×106 cells and crosslinked with 1% formaldehyde (10 min, 37°C). After incubation in cell lysis buffer 30 minutes at 4°C, nuclei were resuspended in nuclei lysis buffer containing glass beads (400 μl per ml) (Sigma) before sonication (4-5 cycles of 4 minutes) using the Biorupter (Diagenode). The INPUT sample (5%) was removed before the IP. Chromatin aliquots (20μg or 50μg for transcription factors) were immunoprecipitated with specific antibodies overnight at 4°C and recovered with pre-blocked Protein G/A sepharose beads (Sigma). Washes were as following: twice TSE-150mM NaCl buffer, once TSE-500mM NaCl buffer, once washing buffer and twice TE buffer. Beads were diluted in 100 μl of dilution buffer plus 150 μl de TE/1% SDS. After de-crosslinking, DNA was analyzed by qPCR. The immunoprecipitation enrichment was calculated using the deltaCt method. Results were presented as "percentage of enrichment" of INPUT sample (100x [Output/Input]) or as "fold-enrichment" (100x [Output/Input] normalized to binding on MMP-9 promoter in Control cells).
Cells were fixed in 70% ethanol and washed twice in PBS, incubated at room temperature for 20 minutes in PI buffer (50 μg/ml propidium iodide; Sigma) containing 100 μg/ml RNaseA. Cells were analyzed using the Facscalibur flow cytometer (BD Biosciences), with data acquisition using CellQuest software.
Zebrafish (Danio rerio) (wild-type AB strain, and casper, generously given by Amagen Paris) were handled according to the European Union guidelines. Fish were kept at 28°C in aquaria with day/night light cycles. HT1080 cells were transfected with siRNA oligonucleotides. After 24 hrs, cells were incubated with D-PBS containing 10 μM fluorescent Cell tracker Green CMFDA (Invitrogen). 20-30 cells were injected into the yolk of dechorionated and anesthetized (Tricaine methanesulfonate) 48 hpf (hours post fertilization) embryos using a Femtojet microinjector (Eppendorf, Hamburg, Germany). Embryos were incubated at 34°C for 3 days and analyzed using a microscope Station cell observer ZEISS and Axio Vision software.
As MMP-9 expression has been linked to Theileria-transformation (7, 15, 27), we analyzed mRNA and protein in Theileria-infected TBL3 cells and the non-infected BL3 parental cell line. We observed high MMP-9 mRNA levels in TBL3 cells, but not in BL3 cells (Figure 1A). To verify reversal by the theilericidal drug Buvarvaquone, we tested different concentrations of Buparvaquone treatment on cell proliferation. The drug caused dramatic growth arrest in TBL3 cells, whereas BL3 growth was unaffected (Figure 1B). Using the standard dose concentration of 50 ng/ml for 64 hours (5), we observed a five-fold reduction in MMP-9 transcript levels in treated TBL3 cells, but no effect in BL3 cells (Figure 1C). Furthermore, we observed a similar Theileria-induction and Buparvaquone-reversibility of MMP-9 protein and protease activity by gelatin zymography (Figures 1D and 1E). Finally, we studied their invasive capacity using in vitro Matrigel invasion assays. TBL3 cells were highly invasive compared to BL3 cells, and Buparvaquone treatment reversed the phenotype (Figure 1F). Thus, the Theileria-inducible and Buparvaquone-reversible regulation of MMP-9 expression offers a model system to explore epigenetic control of this metastasis gene.
To explore epigenetic changes linked to MMP-9 regulation, we studied histone marks on the MMP-9 promoter focusing on a regulatory region (0.5 kb upstream from the TSS containing transcription factor binding sites AP-1, NF-κB, and Sp1) conserved between the bovine and human promoters (Figure 2A and S2). We performed Chromatin Immunoprecipitation (ChIP) studies in TBL3 and BL3 cells, with and without Buparvaquone treatment, using antibodies specific for activating histone marks (i.e. Acetyl-H3, H3K4me2, H3K4me3), and repressive marks (i.e. H3K9me3 and H3K27me3) (Figure 2B-F). We observed enrichment of H3K4me2 and H3K4me3 marks in this region in TBL3 compared to BL3 cells (Figure 2B-2C). Furthermore, Buparvaquone treatment dramatically decreased H3K4me3 marks in TBL3 cells (but not H3K4me2), almost to the levels observed in BL3 cells (Figure 2B). In contrast, we observed no significant changes for Acetyl-H3, nor the repressive H3K27me3 and H3K9me3 marks, upon Theileria infection or Buparvaquone treatment (Figures 2D-2F). Thus, reversible Theileria-induced MMP-9 transcription is associated with dramatic changes in H3K4 tri-methylation on the MMP-9 promoter.
To identify enzymes responsible for the H3K4-methyl marks on the MMP-9 promoter, we examined expression of a panel of H3K4 methyltransferases. Strikingly, SMYD3 was the only methyltransferase gene clearly induced by Theileria in TBL3 cells (over 4-fold induction) (Figure 3A). Furthermore, Buparvaquone treatment decreased SMYD3 expression to BL3 levels (Figure 3A). The bovine SMYD3 gene encodes a protein very similar to the human enzyme, with almost complete conservation of key SET domain residues (Figure S1). To test SMYD3 activity in these two cell lines, we transfected cells with a SMYD3 reporter construct containing the Nkx 2.8 promoter, a known SMYD3 target gene, coupled to Luciferase (24). This SMYD3 reporter was six-fold more active in TBL3 cells (Figure 3B). Furthermore, there was a significant reduction (50%) in the Luciferase levels when the SMYD3-binding site in the promoter was mutated (Figure 3B) (24). Thus, SMYD3 was a promising candidate as the methyltransferase responsible for elevated H3K4me2/me3 marks on the MMP-9 promoter.
SMYD3 binds to DNA via specific binding motifs (24) and bioinformatic analyses identified putative SMYD3 binding motifs in both the human and bovine MMP-9 promoters (Figure 3C, Figure S2). ChIP-qPCR analysis of the bovine MMP-9 promoter, using oligonucleotides that amplify a cluster of putative SMYD3 binding sites, revealed a marked enrichment for H3K4me3 modifications in this region in Theileria-infected cells (Figure 3D). Enrichment was reversed by Buparvaquone treatment and absent in BL3 cells (Figure 3D). These results suggested that SMYD3 binds to sites in the MMP-9 promoter to drive MMP-9 expression. Deeper investigation in TBL3/BL3 cells was hindered by the poor cross-reactivity of commercially-available antibodies with bovine SMYD3.
The putative SMYD3-binding motifs are conserved in the human MMP-9 promoter (Figure 3C, Figure S2), so we investigated the SMYD3-MMP-9 link further in human cancer cells. We studied a fibrosarcoma cell line, HT1080, which expresses MMP-9 and displays high invasive properties in vitro and in vivo (28, 29). We used an HT1080 derivative containing a 2.2 Kb MMP-9-Luc reporter stably integrated into the genome (26) to test SMYD3 effects on promoter activity in a chromosomal context (Figure 4A). We also tested SMYD3 function by transient co-transfection with a MMP-9-Luc reporter plasmid (Figure 4B). In both cases, SMYD3 transfection caused a modest, but statistically significant, increase in MMP-9 promoter activity (1.5-2.5 fold), which was enhanced by PMA treatment (2-5 fold) (Figure 4A-B). We observed similar synergistic effects on MMP-9 protein levels when SMYD3 was transfected with PMA stimulation (Figure 4C). We performed knockdown experiments using two different siRNA oligos that led to marked reduction in SMYD3 mRNA and protein (Figure 5A-5C). SMYD3 knockdown caused a significant decrease in MMP-9 mRNA and protein levels (Figure 5B-5C). Furthermore, siRNA SMYD3 caused a reduction in the activity of the MMP-9-Luc reporter integrated into the HT1080 genome (Figure 5D). These results support the hypothesis that SMYD3 regulates MMP-9 expression in human cancer cells.
We generated stable HT1080 cell lines expressing shRNA against SMYD3 and controls. SMYD3 reduction caused a decrease in MMP-9 mRNA and protein levels (Figure S3A-B). We performed ChIP and observed SMYD3 binding to the MMP-9 promoter in control cells, but not in knockdown cells (Figure 5F). Moreover, SMYD3 inhibition caused a significant decrease in the H3K4me3 marks on the MMP-9 promoter, but not on the control GAPDH promoter (Figure 5F). Also, SMYD3 inhibition did not affect H3-acetylation marks on the MMP-9 promoter (Figure 5F). We excluded the possibility of an indirect effect of SMYD3 knockdown on MMP-9 transcription via the modulation of chromatin modifiers known to participate in the regulation of MMP-9 (22). We found no evidence for altered expression of these regulators upon SMYD3 knockdown (Figure S4). Thus, SMYD3 binds directly to the human MMP-9 promoter and appears responsible for H3K4 methylation and transcriptional activation.
To test whether SMYD3 regulation of MMP-9 contributes to tumor phenotypes, we examined the effect of SMYD3 knockdown using in vitro migration assays with collagen-coated filters. The down-regulation of SMYD3 using two independent siRNAs caused a significant reduction in the migratory capacity of HT1080 cells, similar to knocking down MMP-9 alone (Figure 6A-B). Conversely, we also tested the effect of over-expressing SMYD3 on cell migration; we transfected SMYD3 into human transformed HEK293T cells, which express low levels of endogenous SMYD3 (Figure 6C) and observed an upregulation of MMP-9 mRNA (Figure 6D) and a consequent increase in invasiveness (Figure 6E).
SMYD3 has also been implicated in tumor cell growth (24, 25). We found that knocking down SMYD3 (using siRNA or shRNA) HT1080 cells provoked a decrease in proliferation (Figure S3C), associated with an increased G1 phase and reduced S phase (Figure S3D). Strikingly, SMYD3 knockdown also reduced colony formation in soft-agar growth assays, a standard in vitro test for tumorigenic potential (Figure 7A-D). The few colonies that formed were small, with few dispersed cells (Figure 7C). Moreover, this effect could be phenocopied by depleting MMP-9 (Figure 7A-B), suggesting that these transformed phenotypes are linked to the regulation of MMP-9.
Finally, we tested SMYD3 knockdown in vivo using a zebrafish xenotransplantation model (30) as a simple and effective system for testing cell invasion and metastasis formation of HT1080 cells. The embryos that developed tumors 72 hours post-injection were scored for the dissemination of tumor cells (Figure 7E, Table 1). We detected widely dispersed fluorescent spots in the yolk and in several cases in the tail region of zebrafish embryos in control xenografts, whereas the tumor mass detected in almost all of the embryos injected with SMYD3-siRNA or MMP9-siRNA treated cells exhibit no dissemination (Figure 7E, Table 1). Thus, SMYD3 expression levels affect the invasiveness and metastatic behavior of human tumor cells in vitro and in vivo.
The discovery of a large number of histone lysine methyltransferases and demethylases has revealed that histone methylation is a dynamic process directing the fine regulation of chromatin structure and gene expression (2, 31, 32). Yet it remains a significant challenge to dissect the precise series of epigenetic events in tumor progression, which is critical to develop targeted therapeutic strategies. It is particularly difficult to reconcile the global changes in histone modifications and enzymes, with localized effects on specific gene promoters (33, 34, 35). H3K4-methylation remains particularly intriguing, with examples of deregulated H3K4 methyltransferases (e.g. SMYD3) (24) and demethylases (e.g. PLU-1/JARID1B) (36-38). SMYD3 is interesting in this respect, as it binds to a specific DNA sequence, allowing it to target particular promoters (24). Here, we present evidence that SMYD3 is specifically upregulated upon Theileria-transformation and targets the promoter of the MMP-9 gene. This study offers insight into how Theileria parasites might mold the host cell epigenome and provides the first direct link between SMYD3 and a specific target gene in invasion and metastasis.
We focused on the MMP-9 gene as a paradigm to explore parasite-induced chromatin changes associated with host cell gene expression and invasion. MMP-9 is regulated extensively at the transcriptional level by classical transcription factors such as AP-1 and NF-κB (19). An elegant study by Ma and colleagues showed that these transcription factors lie downstream of signal transduction pathways and are recruited to the MMP-9 promoter in a stepwise process coordinated by chromatin modifying complexes (21). Although AP-1 activation is important for MMP-9 regulation upon Theileria infection, it is not sufficient, as some Theileria-transformed cell lines express AP-1 without activating MMP-9 (data not shown) (14). This drove us to search for epigenetic modulators that contribute to host gene regulation. We found that H3K4me3 was specifically associated with inducible and reversible MMP-9 expression (Figure 2). Surprisingly, we saw no changes in H3-acetylation, previously linked to MMP-9 regulation (21), nor in any repressive histone marks. SMYD3 was the only H3K4 methyltransferase whose expression correlated with the marks observed on the MMP-9 promoter. Our gain-of-function and loss-of-function studies demonstrated that SMYD3 plays a direct role in MMP-9 regulation. This is likely via binding to putative SMYD3 recognition motifs in the MMP-9 promoter and a multiple repeat (5′-CCCTCCCTCCC-3′) that is conserved between the bovine and human MMP-9 promoters (Figure S2). SMYD3 binding to this region appears essential for MMP-9 gene regulation. The effects of SMYD3 knockdown appear to be independent of the expression of other chromatin modifiers (21, 39) (Figure S4). But it is formally possible that SMYD3 and associated H3K4-methylation recruit additional regulatory proteins required for full MMP-9 induction (22). Another possibility is that promoter-bound SMYD3 contributes to gene expression by methylating non-histone proteins locally, such as the AP-1 transcription factor. There are precedents for transcription factor regulation by methylation and this merits further investigation (40). Our study highlights the dynamic nature of H3K4-methylation in transcriptional regulation and how methyltransferase binding to select promoters can control gene expression.
We provide the first evidence that Theileria parasites influence host cell gene expression by modulating epigenetic regulators. The inducible and reversible nature of MMP-9 expression provided a model gene to focus on chromatin events induced by the parasite, leading to the discovery of SMYD3 as a promising candidate. It is likely that other chromatin-modifying enzymes contribute to Theileria-induced transformation, just as multiple modifiers cooperate in human tumors (2). The Theileria system offers an attractive model to search for novel SMYD3 target genes, by comparing infected and Buparvaquone-treated transcriptomes accompanied by genome-wide ChIP analysis. One intriguing aspect is the striking upregulation of solely SMYD3 and not other H3K4 methyltransferases, such as MLL members that are deregulated in human cancers and regulate MMP-9 (41). SMYD3 is overexpressed in human colorectal and hepatocellular carcinomas (24) and breast cancer cells (25). Elevated SMYD3 expression was recently observed in Chronic Lymphocytic Leukemia (CLL) and in highly metastatic pancreatic cancers (42). However, little is known about SMYD3 transcriptional control (43) and our model provides a tool for studying the inducible and reversible regulation of SMYD3 in cancer cells. Our results showing that the SMYD3-MMP9 circuit is functional in human cells, demonstrates that the Theileria model can be effectively used to generate mechanistic hypotheses to be tested in human cancers.
Our study directly links the SMYD3 methyltransferase to the MMP-9 gene, which is important in angiogenesis and metastasis. MMP-9 expression is linked to lymphoma, leukemia and metastasis (44, 45). There are few characterized targets for SMYD3 in tumorigenesis, except for the hTERT telomerase and WNT10B genes (25, 39). Previous studies highlighted the role of SMYD3 in cancer proliferation, but those linking SMYD3 to invasion have not identified a direct molecular target (24, 25, 46, 47). SMYD3 was also identified in gene signatures in metastatic pancreatic tumors (42). The urgent need to develop better therapies for metastatic disease makes it important to identify targets of regulators induced in metastatic cells. Clinical trials using MMP inhibitors have been hampered by problems with selectivity (48). Thus, our finding that a histone methyltransferase, which is induced in metastatic tumors, specifically regulates MMP-9 expression and tumor invasion in vivo, offers a target to develop therapeutic strategies aimed at preventing cancer progression.
We thank Claire Rougeulle and Slimane Ait-Si-Ali for critical reading of the manuscript and members of UMR7216 for helpful discussions. We thank Carmen Garrido for suggesting the zebrafish experiments and André Bouchot for fluorescent microscopy assistance.
Grant Support The Weitzman laboratory was supported by Fondation de France (FdF #2102), Association pour le Recherche contre le Cancer (ARC Fixe #4975, ARC-Equipement #7990) and Association for International Cancer Research (AICR, #08-0111). Langsley laboratory acknowledges funding from Wellcome Trust grant (075820/A/04/Z) and Inserm/CNRS infrastructure support. ACR was supported by Colfuturo (Colombia), ARC and Fondation pour la Recherche Médicale (FRM #FDT20100920170). NJ was supported by Université Paris-Diderot and AXA Research Fund.
Conflicts of Interest: No potential conflicts of interest were disclosed.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
Saturday, July 09, 2016
74 arrested in Rochester, but protests otherwise peaceful – The San Diego Union-Tribune
The San Diego Union-TribuneCALIFORNIA. Dozens of people Saturday blocked a ramp to the Bay Bridge in San Francisco to protest the killings black people by police. About 70 people shouting "Hand up! Don't Shoot!" stood by the entrance to the bridge that connects San Francisco to …Protesters Nationwide Denounce Police ShootingsNBCNews.comNation reacts to shooting deaths […]
from WordPress http://ift.tt/29vvgTt
via IFTTT
from WordPress http://ift.tt/29vvgTt
via IFTTT


text:
In an interactive session at Moneylife Foundation, advocate Bapoo Malcolm explained to a packed house the practical aspects of making a will
Through a Will, we offer specific instructions about what has to be done with movable and immovable properties after our death. As simple as it sounds, there are several misconceptions and many are left confused with the legalities, while preparing a Will. Having conducted many sessions on making a Will in the past, Moneylife Foundation conducted yet another seminar on 'How to write your own Will' at the request of the Foundation members. Like the other events, this event too, received an overwhelming response. Bapoo Malcolm, advocate, Bombay High Court, a man of many interests and the speaker for the event, covered issues related to wills, like registration, litigation possibilities, witnesses, executors, intestate problems and adoption. He also discussed types of wills, including privileged wills, joint wills and holographic wills.
Who can prepare a Will? Mr Malcolm, a well experienced lawyer in both civil and criminal matters, said, "A Will can be made by any person who is not a minor and who is of sound mind. You need two witnesses, preferably independent of each other. The Will must list all the immovable and movable properties you own, and who you wish to will them to after your death." Remember, however, that you can only bequeath what belongs to you and what is self-earned; otherwise the distribution is governed by various Succession Acts.
Explaining the simplicity of creating a Will, Mr Malcolm described that, "A Will is a simple document to be written in simple language. Preferably, avoid legalese. You simply have to give specific instructions about what has to be done with the movable and immovable properties you own and the property you might acquire in future. It would be best if it reads like a balance sheet. Just specify the property and the person." A Will should be legible and clear to understand.
There appear to be several misconceptions about the making of a Will. One was that a Will needs to be registered. Mr Malcolm cleared the doubt. He said, "The government does want to encourage people to make Wills, so they haven't made it compulsory to register a Will. You can even make a Will on a serviette. So long as the basic requirements are fulfilled, the Will is legal. Registration is a grey area, mainly because if you then wish to make a second Will, you may not have the time to register it. If you realise the day you die that a relative is useless, you may not be able register this second Will. Then, does the unregistered Will have more value than the registered one? An unregistered Will could easily be lost; however, registration takes care of the safety issue. At least, if it is registered, you know that it is always in some government ward."
A Will needs to be clear and concise, leaving no room for misinterpretation. "A Will would be interpreted according to the word of the law, which may not assign the same meaning as you intended." For example, while mentioning names it is important to specify the relationship along with the date of birth of the person, as there could be common names. Also, if you wish to intentionally leave someone out of your Will, especially, if it is a close family member, it is better to mention the name in the Will. If not, the person may contest the Will by saying you have forgotten his/ her name, he explained.
Mr Malcolm spoke of the basic components of a Will as well as terms such as testator, executor, codicil, testamentary guardian and a detailed discussion on probate of a Will. He covered issues related to Wills, like registration, litigation possibilities, witnesses, executors, intestate problems. He also answered the queries of Moneylife Foundation members who raised their doubts.
RBI launched inflation indexed bonds will be available till 31st December. The good news is that the rate will be linked to CPI instead of WPI - the less accurate gauge of inflation. The bad news is that early redemption, even after the lock-in period is over, will entail hefty penalty. Should savers bite into the offering?
The Reserve Bank of India (RBI)'s Inflation Indexed National Savings Securities-Cumulative (IINSS-C) securities are open for subscription till 31st December. The central bank is using authorised agency banks and Stock Holding Corporation of India (SHCIL) to invest in IINSS-C. The authorized banks are State Bank of India (SBI) and its associates, all nationalized banks, HDFC Bank, ICICI Bank and Axis Bank.
The Union Budget of 2013-14 had announced the introduction of instruments that will protect retail savings from inflation. These are internationally known as inflation-linked securities or simply linkers. The distribution/sale of linkers would be through banks, for retail investors. Interest rate on these securities would be linked to final combined consumer price index [CPI (Base: 2010=100)]. Interest rate would comprise two parts: fixed rate (1.5%) and inflation rate, based on three-month lag CPI which will be compounded with the principal on a half-yearly basis and paid only at the time of maturity. So, if a bond is being valued in December, the reference rate will be CPI of September. The issuance of non-cumulative inflation indexed bonds for retail investors will be examined in due course. The minimum and maximum investment per annum is Rs5,000 and Rs5 lakh, respectively. The tenor is fixed at 10 years. The new offering should attract higher attention from savers, especially due to its link to CPI instead of wholesale price index (WPI) which is a less accurate gauge of inflation. In June 2013, RBI sold WPI-linked bonds offering a yield of 1.44% above WPI, but the retail response was dismal. In November 2013, the WPI was 7.52% and CPI was 11.24%. The new offering linked to CPI will fetch 12.74% compared to the WPI-linked bonds that would give only 8.96%; a 10-year bank FD gives approximately 9%.
CPI is considered a more accurate gauge of the impact of inflation on consumers because it takes into account increases in the cost of education, food, transportation, housing and medical care; in WPI, the emphasis is on measuring the prices of traded goods and services.
Advantages of IINSS-C
• Inflation has been high in India in recent years; this bodes good returns and protection from inflation by investment in IINSS-C. The safety of the principal should not be a cause of concern as the bonds are a part of the government's borrowing programme. IINSS-C will be considered at par with sovereign rated government securities (G-Secs).
• Even though IINSS-C will be taxed like fixed-deposits (FD), the interest can be substantially higher than FDs during a period of high inflation. Conversely, the returns can go lower than FDs, when inflation is low. FD rates can also go low when inflation dips and, hence, IINSS-C should be preferred over FD, as long as you have a commitment to stay with the product for a long term. For example, CPI of 11.24% in November 2013 means you can expect to get 12.74%pa (per annum) assuming inflation stays at the same level. With inflation levels fluctuating, the returns from IINSS-C will have a zigzag pattern. Scheduled commercial banks offer approximately 9%pa for 10-year FDs which is guaranteed for the full period.
• The product should be easy to purchase from banks; servicing it should not be an issue due to bank involvement. It can be used as collateral for loans from banks, financial Institutions and Non Banking Financial Companies (NBFC). The transferability is limited to nominee(s) on death of holder (only individuals).
• IINSS-C can be used to diversify one's debt portfolio. Their advantage over bonds is that technically the principal is protected in IINSS-C. Selling bonds (taxable or tax-free) during high interest regime can result in getting a price lower than the principal. Early redemption of IINSS-C, after expiry of the lock-in period will ensure that you get back your principal even if inflation falls drastically; but you will take a beating due to the penalty on the coupon rate. It works more like bank FDs which have a penalty on premature withdrawal.
IINSS-C is more like FD, less like a bond. IINSS-C surely has an advantage over taxable and tax-free bonds for the conservative saver who does not like the volatility of the investment amount, based on market interest rates.
• IINSS-C should dissuade savers from buying gold for investment purposes. Beating inflation with gold has been a fallacy; IINSS-C should be an option for this segment as it will actually make your money grow at a rate higher than inflation. It can be an instrument for retirement savings.
Disadvantages of IINSS-C
• The bonds are meant to be long-term savings instruments. They will not be tradable in the secondary market. Liquidity is certainly an issue with IINSS-C; bank FDs are completely liquid; some banks do not even have a penalty on premature withdrawals for all FDs or depending on the tenor of the FD.
• Early redemptions will be allowed after one year from the date of issue for senior citizens (those above 65 years of age) and three years for all others, subject to penalty charges at the rate of 50% of the last coupon payable for early redemption. For example, if you redeem after the third year, you lose half the interest of the previous year. Early redemptions, however, will be made only on coupon dates. Hefty penalty for early redemption is a major deterrent; but bank FDs too have premature withdrawal penalty of 1% lesser interest rate than the rate offered for the period for which the FD was kept. The calculation of bank FD penalty will be similar to IINSS-C penalty and, hence, there is no reason to overlook IINSS-C. If your investment horizon is shorter than three years, you should not go for this instrument.
Read - Inflation Index Bonds Vs Bank FDs – Early redemption penalty decoded
• The Finance Bill of 2012 had lowered the age of senior citizens from 65 years to 60 years for tax benefit purposes. The circular of RBI on IINSS-C, which states that 65 years has to be considered for qualifying as a senior citizen, has brought back the debate on senior citizen's age.
• IINSS-C does not match the charm of public provident fund ( PPF) which allows Rs1 lakh investment in a year for tax deduction under Section 80C. Moreover, interest on PPF is tax free. IINSS-C may not even score over tax-free bonds, especially for savers in the 30% tax bracket. The recent tax-free bonds issues offered coupon rates of 8.66% and 8.76% for AAA and AA+ rated bonds, respectively, of 10-year tenors.
• Rate of interest on IINSS-C will be floating which may not be suitable for those in need of fixed income. Moreover, absence of income flow can make the product unattractive for senior citizens. IINSS-C will have half-yearly compounded cumulative offer of 10-year tenor. Bank FD interest is compounded quarterly for the cumulative as well as non-cumulative option.
• Tax treatment on interest and principal repayment would be as per the extant taxation provisions. Tax will be levied on the interest as per your tax bracket, which is in line with bank FD. The product is suitable for those in the lower tax bracket (up to 10%). It may work out well for those in the 20% tax bracket, if inflation remains at the current level for much of the 10-year tenor. Some media reports talk about considering payment as capital gains which will lower the tax rate. But, ignore such misinterpretation and calculate tax payment, based on your tax slab.
Read - Inflation Index Bonds – How will the taxation impact you?
There are doubts about the true consumer price inflation. If actual inflation is higher, IINSS-C returns will be lower, even in a high inflation regime. Flawed inflation data can be a major worry for IINSS-C investors; but investors, by and large, don't see costs and returns in inflation-adjusted terms.
Kejriwal said he would personally invite Anna Hazare, his guru, but the Gandhian said he may not attend swearing-in ceremony due to his ill-health
Arvind Kejriwal, the founder of Aam Aadmi Party and Delhi's Chief Minister designate on Thursday said he would personally invite his 'guru' Anna Hazare for his swearing-in ceremony at the historic Ramlila Maidan on Saturday.
Speaking with reporters, Kejraiwal said, "Anna Hazare is my guru and I would personally talk to him on phone to invite him for the swearing-in ceremony".
However, Hazare, the social activist when asked if he would travel to Delhi for the ceremony, said, "I am not well". When asked again at in his native Ralegan Siddhi village, Hazare said, "I can't say right now. I am not well".
The Gandhian had said two days back that he is yet to receive an invitation for the swearing-in ceremony but would not attend even if he got one owing to ill-health.
Hazare had earlier declined to comment on the Kejriwal-led AAP's decision to form the new government in Delhi, but added he would express his views when his former protégé acts on the Lokayukta issue.
Hazare had parted ways with Kejriwal following his decision to form a political party.
The relations between the Gandhian and the AAP convener had hit a new low when he recently ordered a frontline leader of Kejriwal's party Gopal Rai to leave his fast venue and village after he engaged in a spat with former Army chief General VK Singh. Singh had slammed Kejriwal and others for having deserted Hazare and formed a political party.
The AAP had immediately recalled Rai from Hazare's fast venue.


text:
TechAspect is HIRING FRESHERS in Hyderabad !!!
Jump Start Your Career.
We are a fast growing company and looking for GenY engineers like you.
Company Name: TechAspect Solutions
Position: 2012, 2013 Passout Freshers Trainee
Education: B.Tech/B.E, MCA, M.Tech
Experience: 0 – 1 Years
Location: Hyderabad / Secunderabad
Job Description:
Apply if you are:
* BE/BTech/MTech/ME with any of these streams (Computer Science / Electronics and Communication / Electronics and Electrical / Mechnical Engineering / Civil Engineering / Instrumentation / Production) OR MCA . 2012 or 2013 passout with 0-1 years of experience.
* Excellent in verbal and written communication skills.
* Holding a minimum of 60% marks in 10th, 12th, and higher education.
See yourself as a part of an exceptional team where everyone can make a difference.
Please let me know if you are interested in this position. If not please refer any of your friends or colleagues who might be looking for a job opportunity.
TechAspect Solutions Pvt. Ltd.
Cura Heights, 2nd Floor, B Block, Plot No -12,
Software Units Layout,
Madhapur, Hyderabad, AP 500081
How to Apply:
Subscribe to "Any Graduate" Job alerts
Subscribe to B.Tech/B.E Job Alerts
Subscribe to "Any Post Graduate" Job Alerts


text:
Southsidedaily.com is your source for free local news and information in Virginia Beach
Ruth "Dorie" LaRock, 85, went to be with the Lord on Oct. 17, 2016.
Born in West Virginia, she was the daughter of the late Hazel and Edith James. She was a lifelong member of Haygood United Methodist Church, founding member of Haygood Preschool, former board member and volunteer for Appalachia Service Project and a lifetime member of the United Methodist Women.
Left to cherish her memory: her husband of 61 years, Frank LaRock; daughters, Brenda Tickle and Edie Crawford and her husband, Donald; sons, Joe LaRock, Jim LaRock and his wife, Pam, and Mike LaRock and his wife, Lisa; grandchildren, Kristie, Greg, Jessica, David, Ryan, Emily, Jessica, Shawn and Jennifer, great-grandchildren, Kiley, Hunter, Cody, McKenna, Savannah, Fotini, and several nieces and nephews. She was predeceased by her sisters Maxine, Virginia and Helen.
The family will receive friends at Hollomon-Brown Funeral Home, Bayside Chapel on Friday, Oct. 21, 2016, from 6:30-8:30 p.m. The memorial service will be conducted at 2 p.m. Saturday, Oct. 22, 2016, at Haygood United Methodist Church by Rev. Tammy Estep. Interment will be private.
In lieu of flowers, memorial donations may be made to Haygood United Methodist Church, 4713 Haygood Rd., Virginia Beach, Va. 23455. Condolences may be offered to the family at www.hollomon-brown.com.


text:
After installing a fresh Etch install, and getting Flash to install, i still had no sound from Flash, via Iceweasel. So, the following walked me through it: http:/
lsof -w $( find /dev -group audio 2>/dev/null )
"If an app uses /dev/dsp* then that one might be the problem." Mine showed "esd" using "/dev/dsp*".
apt-cache policy libesd-alsa0
This showed that the package was NOT installed. So, i installed it!
apt-get install libesd-alsa0
Then logged out, back in, and i Flash sang!