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[Video] So Ji Sub Releases Teaser for 'Eraser' Featuring Park Shin Hye
So Ji Sub finally released a teaser to the anticipated music video featuring Park Shin Hye.
Revealed on January 21, the teaser for Eraser showed Park Shin Hye humming happily in the kitchen until she hears a message from So Ji Sub from the answer machine.
Upon hearing his voice, Park Shin Hye starts to tear up, provoking curiosity to what their story is.
Yoo Seung Ho is also be featured in the music video.
So Ji Sub's second mini album, 6 PM…Ground, will be released on January 23.
Photo Credit: CJ E&M
- Tag
- Park Shin Hye 20130121 So Ji Sub 20130121 Collabo One 20130121


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It rained. We stayed in and watched the sucka free countdown, which, as you probably guessed, is actually full of suckers. However, teenage hyphy freakouts, women's rugby, and their new policy of only playing videos with Pharrell in them has won us over.
Bailey, "U-C-It"
DJ Shadow feat. Turf Talk and Keak the Sneak, "3 Freaks"
Project Pat feat. Three Six Mafia, "Good Googly Moogly" (see also the YouTube exclusive VIDEO SCREW VERSION, like whoa.)
Pharrell and Clipse, "Mr. Me Too"
Pharrell and Kanye West, "Number 1"
Lupe Fiasco, "Kick Push"

subset: commoncrawl


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Case study #2: Exhibition - The Robot Exhibit - Museum of Arts & Design
Hours: 7pm
Ages: All
Price: Free w/ pay-what-you-wish admission
2 Columbus Circle - 212-299-7777
New York, NY 10019
Description: The Robot Exhibit: History, Fantasy, and Reality (1984) at MAD (then the American Craft Museum) was one of the first comprehensive surveys of the history of robots, cyborgs, automatons, and androids. A visionary exhibit that attracted a record number of visitors, MAD presented robots as a multidisciplinary phenomenon, a confluence of craft and science. But beyond the myth of the robot, here were the roots of a revolution -- automatization, new production methods, and even 3D printing and rapid prototyping -- that would take the design world by storm in the following twenty years. How can exhibitions forecast future trends in design, from concepts to manufacturing, funding and consuming? Superscript's programmatic counterpoint to their project On Display brings together the public with voices in the design and architecture world to comment on these topics, and thus activate free posters displayed in the exhibition space of After the Museum.


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A "Pals of the P.A.L." Christmas, 1949
Throughout the 1940's WNYC aired a program titled Pals of the P.A.L., a youth-oriented variety show in cooperation with the Police Athletic League (P.A.L.).
Amidst concerns about juvenile delinquency, the P.A.L. was officially organized in 1936 (though by then the New York City Police Department already had a long history of youth outreach activities). For just 10 cents, a young person could become an official member of the P.A.L., and take advantage of their many offerings, including summertime boat rides, boxing clubs and admission to one of the many indoor centers throughout the city, which offered such activities as "arts and crafts, aquatics, dancing, and dramatics," to name a few.
So far, we at the Annotations have uncovered over a dozen Pals of the P.A.L. episodes, with more awaiting digitization. In essence, it is a program for children by children; all of the children participating in each episode are members of the P.A.L. under the guidance of an adult host. Full of popular songs, silly gags, and short plays performed by the "P.A.L. Players" (and a lot of accordion music), the program provided the children of New York City with a venue to showcase their talents.
In this very special Christmas edition of Pals of the P.A.L. you can hear the musical stylings of P.A.L. members, including everything from "White Christmas" to "Silent Night" and "Ave Maria," as well as a P.A.L. Players original production, The Exposé of Santa Claus as performed by the three sons of Patrolman James Stone: Donald, Billy and Roy (starting at nine minutes in).
If you can't get enough of the P.A.L. performers, fear not! We're planning a spectacular P.A.L. mixtape to be released some time in the new year.
Audio courtesy NYC Municipal Archives collection.


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Hayden Panettiere plays a successful country sensation on "Nashville," and while she may look the part of a music superstar on the ABC drama, she still gets star struck when meeting country legends while filming in the famed Tennessee town.
"I'm beside myself. First of all, I just saw Tim McGraw and Faith Hill and I literally ran across the carpet to hug her," Hayden told Access Hollywood at the 2012 CMA Awards. "She was a doll about it, but I can only imagine what she thought when she saw little old me running across the carpet!"
PLAY IT NOW: From Where Did Hayden Panettiere & Connie Britton Draw Inspiration From For ABC's Nashville?
Adding, "I literally just jumped on Faith Hill. It was the most exciting moment of my life."
The 23-year-old said she's enjoying life in Nashville, as well as the positive response the show has received from the city's residents.
VIEW THE PHOTOS: Fresh Faces Of Fall TV
"I love it. I love that we're doing the show here, I love that we get to really immerse ourselves within the culture of it because Nashville really plays a huge role in our show," she said. "And it's very rare that people get it right. And that's what I've heard more than anything is people from Nashville going, 'Thank you for portraying us in the right light.'"
While the bubbly blonde has been hanging out with country royalty, thanks to her new show, she said she never asks artists if they tune in to see her play Juliette Barnes.
"No, I'm not gonna ask unless they tell!" she said, with a laugh.
VIEW THE PHOTOS: CMA Awards 2012: Country's Best Dressed Beauties!
Access also caught up with Kimberly Williams-Paisley, who made her debut on "Nashville" in last week's episode as Peggy Samper, and the actress hinted that her character will certainly stir the pot in the coming weeks.
"I'm there to cause trouble -- I think there's no question about that -- and not intentionally," she told Access of Peggy, who was seen covertly discussing a past mistake (whether business or romantic?) she made with Teddy Conrad (played by Eric Close). "I think Peggy's made some bad decisions and that's gonna cause some trouble."
VIEW THE PHOTOS: Hayden Panettiere: From TV 'Hero' To Hero Of The Environment
Catch Hayden and Kimberly - along with Connie Britton and Charles Esten - on "Nashville," Wednesdays at 10/9c on ABC.
Copyright 2012 by NBC Universal, Inc. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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Contains over 90,000 entries featuring 10,000 new words and senses, 70,000 audio word pronunciations, 900 full-page color illustrations, language notes and word-root appendices.
Classic Catalog | OhioLINK | Lane Public | WorldCat |
More Options:
Research by Subject |
Databases A-Z
More Options:
OhioLINK Home |
OhioLINK Catalog |
Research by Subject |
Databases A-Z
More Options:
Miami University Search |
Miami University Directory
Find your summer read!
Faculty stipend and workshop offered for exploring open textbooks
Staff Spotlight: Nate Floyd
Celebrate National Poetry Month 2017 with Miami University Libraries


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New Software Notice alert!
The new Web Graphics Creator (version 4.0) will be out soon.
For now, version 3.0 is still active and works great on most Windows machines. It no longer runs on Macs using Lion or greater... (but the new version will fix this)
So hang in there! It's almost done!
Tutorial: Adding text to your 3D eCover
Discussion in 'Web Graphics Creator' started by Mr Laughingbird, Nov 14, 2006.


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Atomico closes on $765 million for its biggest fund yet
Atomico has closed its fourth fund with $765 million, making it one of Europe's single largest venture capital funds. The firm began raising the fund eleven months ago (we'd flagged it for you after searching the SEC filings). And while it represents a sizable step up from Atomico's third fund, which closed with $476.6 million in 2013, its pace of fundraising highlights… Read More
Klarna buys BillPay, the PayPal of Germany, for $75M from Wonga
Some chopping and changing is afoot in the world of payments in Europe. Today, Klarna, the startup out of Sweden that works with online merchants to enable flexible payment options, confirmed that it has acquired BillPay, a payments company based in Germany, from its previous owner Wonga, the startup that once achieved notoriety for predatory payday loans. The companies are not disclosing… Read More
The team behind Cookies is joining Klarna
All the work that went into creating peer-to-peer payment app Cookies isn't disappearing forever. After filing for bankruptcy, Swedish tech company Klarna approached the team behind Cookies so that they can join another fintech company. The entire Cookies team except one co-founder is joining Klarna, as Klarna announced and t3n reported. I don't think the two co-founders can… Read More
Klarna launches its first credit product with new U.S. partnerships
The online financing giant Klarna is continuing its rollout in the U.S. with a host of new partnerships and a new product so online shoppers in the U.S. and Europe can actually take out loans from the company. It's the next step for the Stockholm-based business as it continues on the road toward becoming a global financial player. Klarna already provides "try before you… Read More
Crunch Network
Fintech dominates Nordic startup investments
Between January 1, 2014 and end of March 2016, 51 fintech investments were made in companies in Sweden, Finland, Norway, Denmark and Iceland, totaling $390.17 million. Read More
Klarna and Modo announce a new strategic partnership at Money20/20 Europe
Unicorn eCommerce startup Klarna and digital payments startup Modo announced a new strategic partnership this morning at Money20/20 Europe. Under the terms of this new partnership Modo will provide Klarna with its Digital Payments Hub product to speed up implementation of Klarna's checkout solution for their worldwide e-retail partners. Read More
Crunch Network
Sweden Is A Tech Superstar From The North
While most of Europe has been mired in economic turmoil since 2008, Sweden has managed to stay afloat — and even accelerate its growth. The country and, especially, its capital, Stockholm, have outpaced other European nations with a mix of unique cultural traditions, visionary tech leaders, globally oriented startups and smart government policies. Read More
From Flipping Burgers To A $2.25B Fintech Startup
Klarna co-founder and CEO Sebastian Siemiatkowski took the stage at Disrupt London today to discuss his payment startup and the wider financial tech ecosystem in Europe and in the U.S. Klarna isn't a household name outside of Europe yet. The company only launched there in September, thanks to a partnership with Overstock.com. Still, Siemiatkowski, who met his co-founder when they worked… Read More
Fashion Marketplace Farfetch Raises $86M Led By DST At A $1B Valuation
Some big news today in the world of fashion e-commerce. Farfetch, the London-based online marketplace for high end fashion retailers, is announcing a raise of $86 million. The funding vaults Farfetch into the unicorn club with a valuation of $1 billion, and it is notable for another reason: it is led by DST Global — the VC firm founded by Yuri Milner that has invested… Read More
Klarna Announces U.S. Team As It Plans For 2015 Launch In The U.S.
The payments market in the U.S. couldn't be be more competitive, and now yet another player is entering the mix. Stockholm-based Klarna is set to announce its U.S. team ahead of expansion plans into North America, which will begin in earnest at the beginning of 2015. Read More
Investors Cash Out Of Payment Technology Startups
Even as a clutch of new payment technology companies including Zooz Mobile and Plastiq announce new financing rounds today, it seems like investors are no longer paying out for payment companies. Over the past three quarters, the number of venture-backed payments companies has declined, tumbling from 59 startups in the third quarter of 2013 to just 41 companies in the second quarter of 2014. Read More
Klarna Acquires Germany's Sofort For $150M To Build A Formidable European Payment Network
Klarna, the Stockholm-based payments company that has raised a whopping $250 million from top-tier investors like Sequoia Capital, is making an acquisition of its own. The company, which makes it easy for European consumers to buy goods online before paying, is acquiring Germany's market leader Sofort. Together, they'll have 10 percent market share in greater Europe's… Read More
Why European Enterprise Startups Should (Or Shouldn't) Move To Silicon Valley
Should European enterprise startups stay in Europe or head to Silicon Valley? That's a question our enterprise panel with TechCrunch's Ingrid Lunden, Klarna's Niklas Adalberth, Huddle's Andy McLoughlin and Zendesk's Mikkel Svane at TechCrunch Disrupt Europe in Berlin tackled today. Both Zendesk and Huddle decided to move a large part of their companies to the U.S. Read More
Klarna Execs' Alleged Sexual Assault Case Dismissed (Update)
Remember those two high-level executives from Swedish startup Klarna who were arrested earlier this month on sexual abuse charges? Their names are Jens Saltin and Niklas Adalberth, and they were arrested at the W Hotel in New York after allegedly molesting a 19-year-old tourist from Texas. Well after a little follow up (it's not often that we get to call into the New York City… Read More
UPDATE: Executives Of Swedish Start-Up Klarna Arrested For Alleged Molestation
Details are sparse and we have calls in, but the two execs at Klarna, Jens Saltin and Niklas Adalberth, were arrested at the W Hotel in New York for alleged molestation. The victim was a 19-year-old tourist from Texas. Saltin and Adalberth are currently out on $10,000 bail. According to the NY Post, "Adalberth allegedly straddled her body while he and Saltin ripped off her clothes… Read More
Saul Klein's List Of Europe's Next Billion-Dollar Tech Companies
Where will the next billion-dollar startups come from? The tech world and most VCs tend to be parochial, looking at Silicon Valley, maybe New York, and a few other hot markets like China and Brazil. But what about the Old Country? Yesterday, I was having coffee with Saul Klein, a partner at Index Ventures and co-founder of Seedcamp. He believes that in every major city across Europe… Read More
Paypal Is About To Get A Bruising From Facebook And Square
2011 is going to be a big year for payments, with more startups and mature companies getting funded in the space than almost ever before. It's important to make the distinction between the headline chasers, the slow moving giants struggling for a piece of the pie and the companies that have a chance at real disruption. For my money Facebook and Square are both very interesting companies… Read More
Klarna payments platform secures Sequoia backing and adds Mike Moritz to the board
Klarna, one of the biggest providers in Europe of in-store credit and invoice based payment solutions for the e-commerce sector, has secured funding from Sequoia Capital, which famously also backed Google. Terms have not been disclosed but we've learned Klarna will do a directed new share issue of SEK 70m (approx. $9 million). Highly unusually, Mike Moritz, Sequoia Capital Partner and… Read More
Moritz Joins First Euro Board As Sequoia Backs Klarna's Payments Platform
Klarna, one of the biggest providers in Europe of in-store credit and invoice based payment solutions for the e-commerce sector, has secured funding from Sequoia Capital, which famously also backed Google. Terms have not been disclosed but we've learned Klarna will do a directed new share issue of SEK 70m (approx. $9 million). Highly unusually, Mike Moritz, Sequoia Capital Partner and… Read More


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Qualities That Will Impress Your Boss
10 More Qualities That Will Impress Your Boss
Photo 1 of 11
10 More Qualities That Will Impress Your Boss
There's no magic formula for wowing the boss, but there is a particular set of skills and characteristics that is universally attractive to employers and will serve you well in any workplace. Once you've mastered the 15 qualities most likely to impress the boss, up the ante with even more. Remember: learning and anticipating your manager's needs, understanding her expectations, and talking to her about shared goals are the best ways to get ahead, but you can also get a head start by putting your best foot forward. Here are more helpful suggestions.


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Car Hire Calgary
Please call 0871 422 3910 Please note calls charged at 10ppm plus your network access charge.
Calgary in Canada has become an extremely popular tourist destination for those who love winter sports but it is also developing a large following in ecotourism in the summer months. The Canadian Rockies are just 80 kilometres from the city and many spend some time enjoying the city cosmopolitan feel and lively nightlife and shopping before heading to the smaller resorts in the mountains for some winter sport action! The city itself is large which warrants obtaining transport even if you intend just to be a city dweller for your stay. If you are heading into the amazing surrounding country and mountains then a car rental is an absolute necessity.
There are plenty of car rental companies in Calgary and they can be found scattered around the city and airport. Given that there is huge variation in peoples needs in a car hire it is highly advisable to book your car hire in advance. In the witner months, this hugely popular location means that vehicles suitable for heading into the mountains and dealing with extreme weather will be snapped up very quickly. The last thing that you want at the beginning of your trip is to find that there is no suitable car available for you! Thanks to the Internet it is easy to search and find the perfect rental, book it online and have it waiting ready for you when you arrive. You may also find some great deals online that you would not get as a walk in customer. Whatever your plans for your trip, renting a car will open up Calgary and the beauty of Canada to you that just cannot be missed.


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Hi I had Breast Implants 3 half years ago, Everything was fine Apart from lose of sensation & a little tender at times, The last few weeks iv been having pain in my right breast, My Husband said my right breast now looks smaller than my left breast & he can see a indented line under my right breast, When i Breath in i get a right pain, The only way i can explain it is Where my chest bone is @ the back of my right breast this is where the most pain is. I can feel any lumps or anything,
Breast Implants 3.5 Years Ago - Now Getting Pain and Right Breast is Smaller Than Left
Doctor Answers 1
Change after Breast Augmentation
By now you may have seen a plastic surgeon which is what would be necessary to come up with the correct diagnosis. If you have silicone gel implants, you should consider an MRI to rule out leakage of the implant. Also, encapsulation (capsular contracture) is one of the issues you may be experiencing.
Web reference: http://www.poustiplasticsurgery.com/Procedures/procedure_breastrevision.htm
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


text: ETLO's online professional development program is highlighted in a recent Education Week article about the crucial role that online professional development plays in the implementation the Common Core State Standards. ETLO's director, Barbara Treacy, shares that, "[Teachers] have been teaching a certain way and under certain kinds of standards and objectives for a long time. To change, we're not going to be able to snap our fingers. They need support, and we cannot short-shrift the PD that teachers need." Read the full article by viewing the link below.


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Item: CRL505
60 Wafers
Serving Size 2 wafers
Servings Per Container 30
|Amount Per Serving
|% Daily Value
|Total Carbohydrate
|4 g
|4 g
|Vitamin D (as ergocalciferol)
|Calcium(as calcium carbonate, calcium citrate)
|1,000 mg (1 g)
|Magnesium(as magnesium oxide, magnesium aspartate)
|500 mg
*Percent Daily Values are based on a 2,000 calorie diet.
**Daily Value not established.
Other ingredients: Fructose, stearic acid, mannitol, magnesium stearate, orange flavor, cellulose, silica, vanilla flavor.
Directions: Adults chew two (2) wafers daily. As a reminder, discuss the supplements and medications that you take with your healthcare providers.
WARNING: If you are pregnant or nursing, taking medication or planning a surgery, consult your doctor before using this product. If any adverse reactions occur, stop taking the product and consult your doctor.
No: Wheat, soy, gluten, milk, salt, preservatives, artificial color or artificial sweeteners.
1.42 lbs
Country Life Cal-Snack is a chewable calcium and magnesium supplement that supports strong bones. These wafers have a delicious vanilla and orange flavor. With vitamin D. Does not contain: wheat, soy, gluten, milk, salt, preservatives, artificial color or artificial sweeteners.
"With my personal 100% money-back satisfaction guarantee, you have nothing to lose and great savings to gain."
Lee Swanson, Founder of Swanson Health Products
If you are dissatisfied for any reason, we'll give you a full refund and pay return shipping.
We are dedicated to offering the highest quality products at the best price. Find out more about how we ensure such a high standard in everything we do.Learn More


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The man accused of a hit and run in Hawkes Bay that left a 5-year-old boy in a critical condition last week has failed to appear in court this morning.
Joshua Manukapei Watson, 27, a seasonal worker, is charged with having been a driver of a vehicle involved in an accident and leaving the scene without stopping to ascertain whether anyone was hurt.
His case was called in Hastings District Court this morning but Watson did not appear. A warrant was issued for his arrest.
The young boy, Kayzah-Shae Wairama, was transferred to Starship Hospital in Auckland after the August 13 accident, which happened in Flaxmere.
He was put in an induced coma but listed in a stable condition this morning.
Kayzah-Shae's grandmother, Lovey Winitana Orlowski, was delighted last week with the news that the driver had turned himself into police on Thursday.
"We are all just so relieved, that is what we wanted, we wanted him to hand himself in."
For more articles from this region, go to Hawkes Bay Today


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I was thinking of purchasing the Extractor heat tape from Betterbee and wanted to know if anyone else has used it to warm the extractor.
I thought it might help the honey flow better while extracting in my cool basement. I also thought of just purchasing some of the heat tape used for keeping pipes from freezing. What do you think?


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In the last 90 days, 15 listings have been made available, which means spaces most likley are leasing quickly. You'll want to be prepared to make an offer immediately. There aren't many listings below 2,000 square feet posted, so finding a space for a small business might be tough.
Even though the median size is 3,610 sqft, there are still 0 new listings below 2,000 sqft. Most buildings in this region are approximately 2 stories. Most buildings in this market were built in 2005 and are Class A.
Not much of the real estate is used for commercial space, so it might be hard to find office space. Manufacturing is the dominant industry in this market. Commute by bike is the most popular way to get to work amongst Summit West residents. Commuting between 30 and 45 minutes a day is the norm for those who opt to reside in this part of town.
Trends and status of the commercial real estate market in Summit West.
Market Report
Real time and historical data on commercial real estate listings in Summit West.


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Oct 3, 2012
InRetail Peru was heard with oversubscribed order books Tuesday ahead of today's scheduled IPO pricing, targeting more than $400m. The unit of the Intercorp group operating retail assets including Inkapharma...
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May 24, 2017 | São Paulo, Brazil
Latin America's largest economy continues to navigate a deep recession and the loss ... more
June 1, 2017 | Santo Domingo, Dominican Republic
Central America boasts the highest growth projections in all of LatAm, benefiting ... more
June 8-9, 2017 | Cartagena, Colombia
LatinFinance returns to Colombia to host the region's most important capital markets and investment forum ... more
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The commercial relationship between Latin America and Japan runs deep ... more
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Which area will be most profitable for investment banks in LatAm in 2016?
All material is subject to strictly enforced copyright terms & conditions and cannot be repurposed or reproduced. © 2016 Latin American Financial Publications Inc.


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America's News
Varies by publication.
America's News is a collection of full-text newspapers available online. The database includes 17 Ohio newspapers, plus other major newspapers from other states. Content includes local and regional news, including community events, schools, politics, government policies, cultural activities, local companies, state industries, and people in the community. Paid advertisements are excluded.


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- 2 x eSATA External Connectors
- Up to 3Gb/s
Some manufacturers place restrictions on how details of their products may be communicated.
Easy Installation & OK on JBOD/Passthrough Mode 08/27/2012
This review is from: Koutech IO-PESA220 PCI Express x 1 Low Profile Ready SATA II (3.0Gb/s) Controller Card
This card is being used on my desktop running Win 7 64-bit OS. The card driver was included included in the product CD. Easy installation.
I am using it on passthrough/JBOD mode, with an eSata 4-drive exterior case (Sans Digital Towerraid TR4M). No RAID, just as an eSata connector with port multiplier.
(I am using this card as a replacement for a RocketRaid 622 eSata RAID controller card (from HighPoint Tech) that came bundled with the Sans Digital Towerraid TR4M -- the RocketRaid card was very difficult to configure, and I gave up on it).
Manufacturer's (Koutech) website is hard to navigate, and did not have the latest version of the driver available for download.
Instruction included in the box is minimalist. It talks about using the RAID GUI application to set up JBOD mode, etc. I didn't do that -- Just installed the card and the driver, and connected the external drive case with 2 HDD's, and the card just worked -- it found the HDDs.
Other Thoughts:
Works well so far, connecting my desktop to the external enclosure with 2 drives via eSata on JBOD/Passthrough mode.
I am not using RAID functionality, so can't comment on the same.
2 people found this review helpful. Did you? Yes / No Thanks for the valuable feedback you provided!


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The Original Glowstars Company Glitter Stars Pink
£6.99 Available Ask us a question about this product
Please allow an additional 3 - 6 working days before despatch
This item is coming from a different warehouse and may take slightly longer than usual to despatch. Once the item has been despatched, usual delivery time applies.
Price Drop Notification
The Original Glowstars Company Glitter Stars Pink Product Description
These pretty pink stars and moon create an amazing display in your room whether it be night or day. By day the stars are bright, sparkly and fun but then at night-time, when the lights go out, they produce a gentle glow. The self adhesive pads apply the stars and moon to your walls and ceilings with ease.
- 40 pretty pink sparkling star and moon shapes
- High quality plastic shapes perfect for decorating your child's bedroom
- Create a stunning display for the day then at night the shapes produce a gentle glow
- Includes easy to use self-adhesive pads to stick the stars and moon around your room
Reviews of The Original Glowstars Company Glitter Stars Pink
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You missed it This great deal for Twiggs Salon is over. But don't despair, we've got more great deals every day.
Related Deals That You May Like
Twiggs Salon
Deluxe Treatment Package or $100 of Hair Services at an Upscale, Luxury Salon
Treat yourself to the ultimate afternoon of relaxation and tress-beautifying with these offers from the chic Twiggs Salon, nestled along Lake Street in Wayzata:
• $25 ($103 value) for a deluxe treatment package
• $40 for $100 to spend on hair services with a senior or master stylist
• Chosen as one of the Top 100 Salons by Elle magazine
"Nicole was awesome! Knew what she was talking about and what was best for my hair, as well as understanding what I wanted. So impressed!" - LivingSocial Member
The deluxe treatment package includes a blowout with a chair massage, luxury shampoo, Kerastase conditioning treatment, and a glass of wine.
Enjoy a range of hair services available with a senior or master stylist -- all of which include a chair massage, shampoo, and glass of wine.
PAID VALUE EXPIRES ON May 27, 2022
PROMOTIONAL VALUE EXPIRES ON November 5, 2014


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Holiday Inn Express & Suites Alpharetta - Windward Parkway
Select your stay dates to check availability at Holiday Inn Express & Suites Alpharetta - Windward Parkway
Holiday Inn Express & Suites Alpharetta - Windward Parkway
About the Hotel
Property Location
With a stay at Holiday Inn Express & Suites Alpharetta - Windward Parkway in Alpharetta, you'll be close to KangaZoom and Alpharetta City Pool. This hotel is within close proximity of Wills Park and Verizon Wireless Amphitheatre at Encore Park.
Make yourself at home in one of the 124 air-conditioned rooms featuring refrigerators and microwaves. Your room comes with a pillowtop bed. Wired and wireless Internet access is complimentary, and flat-screen televisions with satellite programming provide entertainment. Private bathrooms with shower/tub combinations feature complimentary toiletries and hair dryers.
Take advantage of recreation opportunities including a fitness center and a seasonal outdoor pool. Additional amenities at this hotel include complimentary wireless Internet access, gift shops/newsstands, and a television in a common area. Getting to nearby attractions is a breeze with the complimentary area shuttle that operates within 5 mi.
A complimentary hot/cold buffet breakfast is served daily.
Business, Other Amenities
Featured amenities include a 24-hour business center, express check-out, and complimentary newspapers in the lobby. Event facilities at this hotel consist of conference space and meeting rooms. Free self parking is available onsite.
Hotel Facilities
- Accessible bathroom
- Free self parking
- Elevator/lift
- Year Built 1989
- Number of buildings/towers - 1
- Laundry facilities
- Number of floors - 6
- Safe-deposit box at front desk
- Free newspapers in lobby
- Smoke-free property
- Conference space
- Dry cleaning/laundry service
- 24-hour front desk
- 24-hour business center
- Outdoor seasonal pool
- In-room accessibility
- Meeting rooms
- Express check-out
- Fitness facilities
- Television in common areas
- Free breakfast
- Free area shuttle
- Multilingual staff
- Total number of rooms - 124
- Gift shops or newsstand
- Coffee/tea in common areas
- Free WiFi
Room Facilities
- Phone
- Free toiletries
- Air conditioning
- Desk
- Private bathroom
- Daily housekeeping
- Free wired high-speed Internet
- Free cribs/infant beds
- Satellite TV service
- Iron/ironing board
- Premium bedding
- In-room climate control (air conditioning)
- Refrigerator
- Pillowtop mattress
- MP3 docking station
- Bathrobes
- Microwave
- Shower/tub combination
- Premium TV channels
- Flat-panel TV
- Free WiFi
- Coffee/tea maker
- Blackout drapes/curtains
- Free newspaper
- Hair dryer
Hotel Policy
Know Before You Go
- Children 18 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.


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The PCT staff has been busy this past year reporting on industry news. Here's a look at the most downloaded news, features and multimedia files that appeared on PCT Online in 2013 broken down by:
News — daily news stories posted by the PCT staff
Features — feature stories that originally appear in the pages of PCT magazine
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Low vision rehabilitation therapy at Oleander Rehabilitation helps those recovering from telescopic implant eye surgery regain independence and learn to perform daily tasks again.
Telescopic implant surgery is a procedure in which a small telescope is placed into the eye to restore sight in those with age-related macular degeneration (AMD), a leading cause of vision loss and blindness among people age 65 and older. The implant takes the place of the patient's own lens and remains in the eye permanently, helping improve vision.
Therapists at Oleander Rehabilitation work with patients, training them to make the eye with the telescope the dominant eye, so they can get back to seeing their families, managing household tasks, and enjoying their lives.


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5 Year Old Cancer Survivor Scores Touchdown At Cleveland Browns Scrimmage
Pretty cool moment out in Cleveland this weekend.
"Ryan Encinas, a 5-year-old from Cuyahoga Falls who recently celebrated two years in remission after battling a cancerous tumor in his left lung, got in the huddle, then took a handoff from quarterback Brandon Weeden. With running back Trent Richardson helping out, Encinas ran through the defense for a touchdown with more than 24,000 fans at FirstEnergy Stadium cheering him on".
Incredible TD run by young Ryan Encinas during #Browns #FamilyNight who's battling a left lung tumor. WATCH: https://t.co/yprZoZogcO
— Cleveland Browns (@Browns) August 4, 2013
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CADM1 isoforms differentially regulate human mast cell survival and homotypic adhesion
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DOI: 10.1007/s00018-012-0948-y
- Cite this article as:
- Moiseeva, E.P., Leyland, M.L. & Bradding, P. Cell. Mol. Life Sci. (2012) 69: 2751. doi:10.1007/s00018-012-0948-y
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Cell adhesion molecule 1 (CADM1), expressed by human lung mast cells (HLMCs), mediates their adhesion to airway smooth muscle (ASM), and contributes to ASM-dependent HLMC proliferation and survival. CADM1 is expressed in alternatively spliced isoforms, but those present in HLMCs and their function are not known. We cloned three functional and one cryptic non-functional isoform with alternative splicing between exons 7/11 and 1/2, respectively, from HLMCs and human MC lines (HMC-1 and LAD2). Differentiated HLMCs and LAD2 cells expressed the functional isoform SP4 containing exons 7/8/11 (~80% of clones), as well as SP1 (exons 7/8/9/11) and a novel SP6 (exons 7/8/9/10/11). In contrast, immature HMC-1 cells expressed only functional SP4. SP4 overexpression in HMC-1 cells and HLMCs augmented homotypic adhesion to a greater extent than SP1 in various conditions. In contrast, CADM1 downregulation abolished homotypic adhesion, indicating that CADM1 is the sole receptor mediating mast cell aggregation. CADM1-mediated adhesion was enhanced by the presence of cell survival factors. SP1 overexpression in HMC-1 cells compromised survival compared to SP4 overexpression or control. CADM1 downregulation resulted in reduced viability and decreased expression of the pro-survival protein Mcl-1L, but not Blc-2 or Bcl-XL, and increased caspase-3/7 activity in both HMC-1 cells and HLMCs. This coincided with decreased basal Kit levels in HLMCs. In summary, human MCs express multiple CADM1 isoforms which exhibit differential regulation of survival and homotypic adhesion. The most highly expressed SP4 isoform is likely to contribute to MC aggregation and longevity in mastocytosis, and augment the pathophysiology of allergic diseases.
KeywordsHuman Mast cells Adhesion molecules Apoptosis Allergy Signal transduction Lung
Airway smooth muscle
Cell adhesion molecule 1
- HLMCs
Human lung mast cells
- MCs
Mast cells
Alternatively spliced isoform
Tumour suppressor of lung cancer 1
Mast cells (MCs) play a critical role in the initiation of inflammation as a primary defence mechanism against pathogens, but their chronic activation also contributes to the pathophysiology of numerous diverse diseases including autoimmune disorders, allergy and asthma [1]. Human lung MCs (HLMCs) contribute to both inflammation and tissue remodelling in lung diseases such as asthma and pulmonary fibrosis [2], and their relocation within diseased lung tissue facilitates their interactions with structural cells such as airway epithelial cells, fibroblasts and airway smooth muscle (ASM) cells.
Adhesive interactions between cells are a fundamental mechanism of cell communication, permitting the accurate delivery of specific cell–cell signals. In constitutively adherent cell types, they are also critical for survival by regulating a specific form of apoptotic death, anoikis [3]. However, MCs, like other cells of haematopoietic origin, can survive and function in both adherent and suspended states. Cell adhesion molecule 1 (CADM1) is an immunoglobulin superfamily adhesion receptor which mediates adhesion through either homophilic or heterophilic molecular interactions. It is expressed on human MCs and mediates HLMC adhesion to ASM cells in a heterophilic manner; in turn, this direct contact drives HLMC proliferation and maintains their survival [4, 5]. In mousetg/tg MCs, heterophilic CADM1-mediated adhesion supports their survival in the peritoneal cavity and adhesion to fibroblasts [6, 7]. CADM1 therefore appears to play a key role in MC biology.
CADM1 is non-redundant in human physiology and has been implicated in several diseases including cancer, autism spectrum disorder and venous thrombosis [8, 9, 10]. CADM1 expression is significantly reduced in several cancers and inversely correlated with the disease progression [11, 12, 13]. In contrast, CADM1 is upregulated in acute T cell leukaemia and contributes to tissue infiltration [14, 15]. Human CADM1 was originally identified as a tumour suppressor of lung cancer 1 (TSLC1) [16]. Alternative splicing between exons 7 and 11 of the CADM1 gene is found in several species. Bioinformatics analysis of the CADM1 gene and cDNAs, conducted by the NCBI, Ensembl and by Biederer [17, 18, 19], identified alternatively spliced isoforms (SP) in several species with common SP4 (TSLC1, containing exons 7/8/11) and SP3 isoforms (containing exons 7/11), named here following Biederer [19]. In addition, cDNAs for human SP2 (with exons 7/9/10/11) and SP1 (exons 7/8/9/11) are present in DNA databases. Complicating the matter, CADM1 is a highly glycosylated protein (with a protein core of ~50 kDa) with N-glycosylation mapped to IgG domains (~25 kDa) and unmapped O-glycosylation (~25 kDa) [6, 20, 21]. Murine cultured MCs express SP4 and soluble CADM1 (sCADM1 with exon 7/intron 7) [22], but the isoforms expressed by human MCs and their individual roles are unknown.
Final MC differentiation is governed by the local environment within the tissue where MCs reside [23], whereas the majority of studies on MC physiology are conducted using MCs differentiated in cell culture. In addition, major phenotypic and functional differences are found between human and mouse mast cells [24]. The aim of this study was to characterise CADM1 expression and its role in the homotypic MC adhesion and survival in differentiated primary HLMCs and the neoplastic MC lines, HMC-1 and LAD2 [25, 26], derived from patients with MC leukaemia/sarcoma. We found that CADM1 is expressed as two major isoforms, SP4 and SP1, in differentiated human MCs and that these isoforms differentially regulate homotypic MC adhesion and survival.
Materials and methods
Cell culture
The human MC line HMC-1.1 (V560G) was cultured in Iscove's Modified Dulbecco's Medium (IMDM) with 10% foetal calf serum (FCS) as described previously [27]. HLMCs were isolated from normal lung obtained at surgery for carcinoma by selection using anti-CD117-coated Dynabeads [27]. The study was approved by the Leicestershire Research Ethics Committee and all participants gave written informed consent. Final MC purity was >99%. HLMCs were cultured in Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 10% FCS, and 100 ng/ml stem cell factor (SCF), 50 ng/ml IL-6, and 10 ng/ml IL-10 as described previously [5]. Freshly isolated HLMCs were used for cloning and to study CADM1 expression. Stabilised 1- to 2-week-old HLMCs, adapted to cell culture, were used for adenoviral transduction.
Primary antibodies
Anti-CADM1 3E1 IgY mAb was from Medical and Biological Laboratories, Japan. Anti-KIT (E1) mAb and anti-β-actin HRP-conjugated antibody were from Santa Cruz Biotechnology. Antibodies against KIT (mAb Ab81), Bim (mAb C34C5), Bcl-2 (mAb 50E3), Bcl-xL (mAb 54H6) and Mcl-1 (polyclonal #4572) were purchased from New England Biolabs.
Protein analysis
Surface CADM1 expression was measured using FACS analysis of non-permeabilised cells. Cells were fixed in 4% paraformaldehyde in PBS and stained with anti-CADM1 mAb or control IgY, followed by FITC-conjugated anti-IgY Ab (Fisher Scientific) and then examined using a FACSCanto with FACSDiva2 software (BD Biosciences, USA). Since some CADM1 peaks were asymmetric, geometric means were used as a measure of the surface CADM1. Total protein analysis was conducted as previously described [28]. SDS-PAGE and immunoblotting were performed using the NuPAGE electrophoresis system (Invitrogen, USA). Blots were cut into horizontal strips with proteins of a close range of molecular weights, probed with appropriate antibodies. Alternatively, whole blots were sequentially probed with antibodies from different species. Protein bands on exposed films were quantified using a Syngene image system (Syngene, UK).
Quantification of gene expression
All kits for RNA isolation and cDNA synthesis and Taqman probes (CADM1, β-actin, and 18S rRNA) were purchased from Applied Biosystems, USA. mRNA levels for CADM1, β-actin, and 18S rRNA were quantified using MX3000 thermocycler (Stratagene, USA) as previously described [28]. The relative levels of CADM1 expression were calculated by the 2−ΔCT method [29].
Cloning and analysis of CADM1 isoforms
List of oligonucleotides
Sequence 5′→3′
Location in the CADM1 gene
5′UTR, exon 1
Overlapping initiation codon (ATG), exon 1
Exon 1F
Exon 1
Exon 2R
Exon 2
Exon 7F
Exon 7
Exon 11R
Exon 11
Exon 12, 3′UTR
Overlapping stop codon (TAG), exon 12
EcoRI site in 5′ to the initiation codon, exon 1
AgeI site in 3′ to codon 442, exon 12
Modulation of CADM1 expression
SP4 cDNA was re-amplified using oligonucleotides 5′CADM1 and CADM1–5′GFP, cut with EcoRI and AgeI restrictases and cloned in frame into EcoRI–AgeI-cleaved pEGFP-N1 plasmid (Clontech, USA) to express CADM1–GFP-fusion protein. The fragments for CADM1 cDNAs, obtained by KpnI–NotI restriction of respective plasmids, for SP4 (p24), CADM1–GFP (pCADM1-EGFP) and SP1 (p33) were recloned into a vector, provided by BioFocus (Netherlands, Leiden), and packed in Ad5C20Att01 adenovirus by BioFocus. Packaged adenoviruses were then purchased from BioFocus. All recloned cDNAs were sequenced to verify correct cloning. CADM1 sh RNAs (Sh3, Sh4, Sh5) were designed by BioFocus outside areas of alternative splicing. Luciferase shRNA (LucSh) was used as a control in downregulation experiments.
Adenoviruses containing SP4–GFP (see Fig. 5b) and GFP were used to optimise transduction conditions according to the manufacturer's recommendations. Expression of CADM1–GFP or GFP in HMC-1 cells 6 days post transduction resulted in visible expression at variable levels in 70 ± 15 and 69 ± 8% of cells, respectively, as previously reported [30]. Similarly, transduction of various HLMCs with these viruses for 6 days produced stable and reproducible fluorescence. Transduction of HMC-1 cells and HLMCs with a multiplicity of infection of 50 IU/cell (17 × 3 IU/cell for mixed sh RNAs) for 6 days were used in all experiments, unless stated otherwise.
Homophilic cell adhesion
Cells, dispersed as a single cell suspension, were cultured in 96-well plates for 3, 18 or 48 h in a cell incubator. Images were then captured on an Olympus CKX42 microscope (Olympus, UK). They were viewed and analysed using CellF imaging software (Olympus). The aggregates were outlined on an image and cross-sectional areas of the largest ten aggregates per photograph of a well were expressed in pixels2. The size of cell aggregates in every condition was estimated as the mean of the ten largest aggregates/well in four wells.
Cell viability
Cell viability was examined by measuring cell number and caspase-3 activity as described previously [28]. Cell number was estimated using the ATPlite 1step kit (Perkin-Elmer, USA) using a calibration curve with a known number of HMC-1 cells, with luminescence detected in a plate reader. Caspase-3 activity was measured as accumulation of a specific fluorogenic substrate using the Apo-one Homogeneous Caspase-3/7 assay (Promega, USA), detected in a plate reader and expressed in fluorescent units/cell (FU/cell).
Data analysis
All analysis was performed using GraphPad Prism 5 software (GraphPad Software, USA). All data are presented as the mean ± SE. Differences among the groups were analysed using a one-way ANOVA, followed by Dunnett's test to determine whether the groups were different from a control group, or Bonferroni's test to compare multiple groups. A repeated measures ANOVA was used to compare multiple data from the same donors. The t test was used to determine differences between two groups. Pearson's and Spearman's tests were used to analyse correlations. P < 0.05 was selected as the level of statistical significance.
CADM1 is highly expressed in HMC-1 cells
Several CADM1 isoforms are expressed in human mast cells
In order to investigate functions of individual isoforms, we used adenoviral delivery of SP4 and SP1, which represent the majority of CADM1 in differentiated HLMCs. Significant modulation of CADM1 expression was achieved by delivery of SP4, SP1 and sh CADM1 RNAs. Surface expression of SP4 and SP1 was increased up to 206 ± 10 and 148 ± 4%, respectively, compared to control HMC-1 cells (Fig. 2b). CADM1 Sh5 and mixed Sh RNAs markedly reduced CADM1 to 41 ± 3 and 25 ± 2% of control, respectively (Fig. 2b). CADM1 downregulation with both Sh5 and/or mixed Sh RNAs was used in further experiments. Western blotting confirmed that modulation of surface CADM1 was paralleled by changes in total CADM1 protein in transduced HMC-1 cells (Fig. 2c). SP1 consistently demonstrated a slightly higher molecular weight compared to SP4 [see Figs. 2c and 5c (below) at 0 h]. Furthermore, SP4–GFP expression influenced expression and glycosylation of endogenous CADM1 (Fig. 2c). No changes in the total amount of Kit or β-actin were observed. Similar CADM1 modulation was achieved in HLMCs [shown in Fig. 6b (below)].
CADM1 mediates homotypic human mast cell adhesion
Because SP4 overexpression in HLMCs led to the formation of large aggregates which maintained cell adhesion in growth medium even after multiple pipetting (Supplemental Fig. 1), HLMCs were transduced for a shorter time (4 days) to examine cell aggregation (Fig. 3a; Supplemental Fig. 2). Both SP4- and SP1-transduced HLMCs formed larger aggregates than control GFP-transduced HLMCs after 3 h (Fig. 3a, bottom panel). However, SP1 aggregates contained fewer cells than SP4 aggregates and were not statistically different from control aggregates (Fig. 3b). Sh5 RNA-transduced cells rarely formed aggregates, whereas control LucSh-transduced HLMCs formed occasional small aggregates, as did GFP-transduced cells (Fig. 3a, b). Hence, the SP4 isoform, in contrast to SP1, promoted fast cell–cell adhesion in both HMC-1 cells and HLMCs.
Aggregation of HLMCs was also studied. A diluted "50% growth medium" (50% HLMC medium/50% IMDM) was used to reduce the potential effect of proliferation. Transduction with either control GFP or LucSh viruses did not change cell aggregation (Fig. 4c). Non-transduced or control GFP/LucSh-transduced HLMCs adhered to plastic and formed small aggregates in "50% growth medium" after 72 h (Fig. 4c). The portion of HLMCs, which strongly adhered to plastic and spread on it, varied in HLMCs from different donors (compare left and right panels in Fig. 4c). SP4-overexpressing HLMCs formed larger aggregates compared to control cells in the same conditions (Fig. 4c) similar to SP4-overexpressing HMC-1 cells. In contrast, HLMCs with downregulated CADM1 were present mostly as single round cells and elongated cells adherent to plastic in "50% growth medium", similar to control, but only as single round cells in IMDM alone. Paradoxically, CADM1 downregulation in some HLMCs appeared to reduce adhesion to plastic in IMDM, suggesting a crosstalk between CADM1 and other adhesion receptors. Altogether, our data suggested that CADM1 is responsible for homotypic cell adhesion of human MCs and CADM1-mediated adhesion is influenced by survival signalling in both HMC-1 cells and HLMCs.
β2-Integrin and ICAM-1 have been implicated in the homotypic adhesion of HMC-1 cells and human cultured MCs [31, 32]. Moreover, MCs express E-cadherin [33, 34], which may also contribute to aggregation. Hence, we examined MC aggregation in the presence of EGTA and EDTA to inhibit divalent cation-dependent aggregation via β2-integrins or cadherins. Neither EGTA nor EDTA affected aggregation of HLMCs cells after 3 h (Supplemental Fig. 2 top panel; EDTA data are not shown). SP4-overexpressing HLMC aggregates were not affected by incubation in EGTA or EDTA for 3 or 18 h; hence, neither integrins nor cadherins were involved in HLMC homotypic adhesion. HLMCs with downregulated CADM1 formed loose aggregates after 18 h only in the absence of EGTA/EDTA in contrast to non-transduced cells (Supplemental Fig. 2). Aggregation of control HMC-1 cells or cells transduced with SP4 was not affected by either EDTA or EGTA after 3 or 18 h (Supplemental Fig. 2 bottom panel). Thus, homophilic CADM1-dependent adhesion is exclusively responsible for homotypic cell–cell adhesion in MCs.
SP1 over-expression and CADM1 downregulation compromise HMC-1 cell viability in the absence of survival factors
Apoptosis-related protein expression was examined in HMC-1 cells deprived of serum. In particular, we were interested in Mcl-1 (myeloid cell leukaemia 1) and Bim (Bcl-2 interacting mediator of cell death) in regard to their roles in survival of mast cells in vivo and systemic mastocytosis [35, 36, 37]. After transduction for 6 days, protein levels were measured at baseline (0 h) and 44 h later in IMDM alone (Fig. 5c, d). CADM1 protein expression increased in control non-transduced, GFP- and LucSh-transduced cells 44 h after the media change (removal of serum), representing a possible compensatory response aimed at maintaining survival. CADM1 increased in Sh5/Shm-transduced cells due to re-expression of endogenous CADM1 after removal of transducing viruses (Fig. 5c). Pro-apoptotic Bim was upregulated at 44 h post removal of serum and viruses in some cells: particularly in Sh5-transduced cells, indicating the onset of apoptosis. Anti-apoptotic Mcl-1 was markedly increased at 44 h post removal of serum and viruses, and appeared as a strong doublet band, as described previously [35]; the size of the shorter Mcl-1 band (≥36 kDa) suggested that it was likely to be phosphorylated rather than alternatively spliced or cut by caspase-3 [38]. Modifications of Mcl-1 protein affect its function, such as protein stability and/or interactions with Bim [38]. Identification of the Mcl-1 shorter band was beyond scope of this study. Unexpectedly, when protein levels at the end of the viral transduction period (0 h) were compared between CADM1 downregulation and controls, there was a reproducible reduction of Mcl-1 to 68 ± 2 and 57 ± 1% in the Sh5 and Shm groups, respectively (Fig. 5d). Mcl-1 band densities in combined downregulation (Sh5 + Shm) were decreased compared to the combined control (none + LucSh) group (P < 0.001, n = 4). Furthermore, there was a correlation between CADM1 and Mcl-1 levels in HMC-1 cells with modulated CADM1 and regression analysis confirmed that CADM1 amounts predicted Mcl-1 levels in HMC-1 cells (Fig. 5d, e). Thus, several lines of evidence confirm a link between CADM1 and survival in HMC-1 cells.
CADM1 downregulation compromises HLMC viability in the absence of survival factors and modulates expression of Kit and Mcl-1
HLMC specimens were pooled to obtain sufficient amounts of proteins for analysis. Analysis of two pools showed different isoforms of Mcl-1 and Bcl-X proteins, compared to HMC-1 cells, with isoforms indicated in Fig. 6b as published previously [39, 40]. In addition to the pro-survival Mcl-1L, HLMCs expressed the pro-apoptotic Mcl-1ES. Bim levels were very low in HLMCs and detectable only in one pool. CADM1 upregulation decreased pro-apoptotic Mcl-1ES to 24 ± 16% in both pools and decreased Bim in one pool. CADM1 downregulation resulted in marked decreases in basal levels of Kit and Mcl-1L to 29 ± 1 and 15 ± 9%, respectively, in both pools. No significant changes were observed in HLMCs transduced with control GFP or LucSh viruses (Fig. 6d). In addition, pro-apoptotic BimL was detected only in the Sh5-transduced pool of cells. Protein quantification showed a strong positive correlation between CADM1 and Mcl-1L levels (Fig. 6c). Hence, CADM1 modulation induced visible changes in basal pro-survival/apoptosis protein levels. Overall, several lines of evidence indicated that CADM1 is an important survival factor in human MCs.
Much less is known about human CADM1 compared to mouse CADM1 despite its involvement in several diseases. For the first time, we have delineated the profile of CADM1 isoforms in human MCs, and the role of CADM1 in human MC homotypic adhesion and survival. Our data also revealed a link between CADM1 and Mcl-1, but not other Bcl-2 family proteins. In contrast to murine MCs which express only the SP4 and sCADM1 isoforms [6, 22], human MCs express several functional variants including SP4, SP1 and SP6, but not soluble CADM1 (not shown). In addition, we identified novel SP6 and non-functional cryptic c15 isoforms, which have not been described previously. Since murine MCs significantly differ from human MCs in many respects [24], it is possible that CADM1 expression may contribute to some of the observed phenotypic differences.
Although the SP4 isoform is prevalent in all human MCs, the longer SP1 and novel SP6 isoforms were found only in highly differentiated HLMCs. Interestingly, all cloned isoforms contained exon 8, encoding 17 Thr codons out of a total of 28. The NetOGlyc 3.1 server [41] predicts that the only O-glycosylation sites in CADM1 are all 17 Thr in exon 8 and 4 Thr in exon 9. In agreement with the data on CADM1 glycosylation, the SP4 protein core is ~50 kDa, but it runs at about 105 kDa in western blots and is expected to be both N- and O-glycosylated. Considering that differences between SP4 and SP1 do not exceed ~4–8 kDa (1.1 kDa for amino acid residues encoded by exon 9 + four additional O-glycosylation units with molecular weight about 3.2–6.4 kg/mol [42]), these isoforms are not easily separated in SDS-PAGE. Similarly, SP6 is just ~1 kDa heavier than SP1. Supporting the prediction, overexpressed SP1 had a slightly higher molecular weight compared to SP4 in western blots. The significance of CADM1 O-glycosylation is not established, but it is feasible that glycosylated threonines form a stiff stalk (2.5 Å per each glycosylated residue [41]) in the extracellular domain which moves the first IgG domain, involved in cell adhesion, further away from cell membrane. This extension might facilitate adhesion, which could be important for interactions of HLMCs with structural airway cells.
Homotypic adhesion of human MCs is suggested to be mediated by β2-integrins [31, 32]. In our experiments, there was little evidence for integrin-dependent homotypic adhesion. In contrast, downregulation of CADM1 both in HLMCs and HMC-1 cells markedly reduced homotypic MC adhesion in normal growth medium and abolished it in the absence of survival factors. CADM1 is, therefore, a key receptor mediating homotypic adhesion in both leukaemic MCs and differentiated HLMCs. The strength of CADM1-mediated adhesion was influenced by other factors such as growth conditions and cell type (cells in SP4-transduced HLMC aggregates did not separate after 6 days, but did in HMC-1 aggregates), suggesting that cross-signalling plays a role in CADM1-dependent adhesion. In addition, interactions between CADM1 and Kit may influence adhesion [5]. A connection between CADM1 and Kit was particularly obvious in HLMCs with downregulated CADM1 (Fig. 6b), where a reduction in CADM1 was paralleled by a reduction in Kit.
Abnormal accumulation of MC aggregates in various tissues is characteristic of advanced mastocytosis and MC accumulation in bone marrow on the background of systemic mastocytosis is a major diagnostic factor for MC leukaemia [43]. Leukaemic HMC-1 cells express 4.5-fold more CADM1 than HLMCs, suggesting that CADM1 may be involved in MC aggregation in this disease. Furthermore, the D816V KIT mutation present in the majority of patients with mastocytosis is not adequate to induce aggressive MC disorders in isolation [44]. We propose that upregulation of CADM1 SP4 may be an important co-factor. This raises the possibility that MC leukaemia is analogous to T cell leukaemia with high CADM1 levels required for malignant transformation and tissue infiltration [14, 15].
In support of the above hypothesis, the effect of CADM1 on survival was striking both in HLMCs and in HMC-1 cells. SP4 overexpression did not affect survival or caspase-3/7-activity in HMC-1 cells, but increased survival in HLMCs, indicating that the relatively high CADM1 SP4 only levels in leukaemic HMC-1 cells are already sufficient to impart a survival benefit. Conversely, CADM1 downregulation reduced survival, increased caspase activity and decreased Mcl-1, which is a survival factor for MCs [35, 36] in both HLMCs and in HMC-1 cells, suggesting a critical role in MC survival. Thus, reduced viability in cells with downregulated CADM1 may be caused by reduced expression of Mcl-1, which would affect the balance between pro-survival and pro-apoptotic Bcl-2 family proteins impinging on cell viability [45]. The levels of caspase-3/7 activity upon withdrawal of survival factors in HLMCs were considerably higher than those in HMC-1 cells, indicating that these leukaemic cells have high anti-apoptotic signalling. In addition, there were striking differences between HMC-1 cells and HLMCs in basal levels of proteins involved in cell survival, such as Mcl-1, Bcl-X and Bim. Moreover, the differences were not only in the levels of expression, but in the spectrum of expressed isoforms.
In addition, SP1 overexpression reduced HMC-1 survival, which implies that it may exert dominant-negative activity over SP4, and may explain why HMC-1 cells only express functional SP4. Interestingly, leukaemic LAD2 cells, expressing both SP4 and SP1, are less robust, proliferate slowly, and require additional factors for growth and survival [46]. In the case of SP1, we did not find specific changes in Mcl-1 expression or any others to indicate the mechanisms of reduced survival in SP1-overexpressing MCs. It has been suggested that the presence of exon 9 in CADM1, which is present in SP1, results in constitutive shedding of the CADM1 extracellular domain and an increased presence of the intracellular domain within cells [47]; this is likely to impact on cell signalling and may affect CADM1-dependent survival.
Although the SP4 CADM1 isoform promotes both MC homotypic adhesion and survival, these two processes do not appear to be directly related. In the absence of serum, control non-transduced HMC-1 cells do not aggregate, but still survive to a similar degree as SP4-transduced cells which form large aggregates. In contrast, SP1-overexpressing cells do aggregate but survive to a lesser degree than control cells, and CADM1 downregulated cells which do not aggregate have impaired survival. It is therefore possible to have several scenarios: increased SP1-dependent adhesion with reduced survival, maximal adhesion in SP4-overexpressing cells with maximal survival, medium strength adhesion with maximal survival in constitutive SP4-expressing HMC-1 cells, and minimal adhesion in CADM1-downregulated cells with reduced survival. This suggests that CADM1 SP4 and SP1 deliver survival signalling independently of their role in cell aggregation and that CADM1-dependent MC survival does not appear to be dependent on CADM1-dependent adhesion. In this respect, CADM1 is similar to other immunoglobulin-like CAMs (cell adhesion molecules), such as NCAM, which can activate signal transduction cascades in the absence of cell adhesion [48].
Our data show that CADM1 plays a key role in MC aggregation and survival and, therefore, is likely to contribute to both clonal expansion and aggregation of MCs in advanced mastocytosis. Our data indicate that SP4 is a survival factor for MCs, and it is therefore likely to be of particular importance for MC survival when other survival signals are unavailable. HLMCs, as a first line for immune defence against infections and pathogens, are characterised by a fast response to the changing environment and relocation into different lung compartments in disease [2]. CADM1 may provide anti-apoptotic signalling during these physiological changes, which involve disassembly of survival signalling during cell migration and relocation. CADM1 may also contribute to the longevity of MCs and, thereby, augment pathology of disorders such as asthma and allergy.
CADM1 is an attractive target for the modulation of MC-dependent disease. Targeting CADM1-dependent adhesion directly may not be a useful therapeutic approach because of its importance in other cell types, but CADM1-induced signalling is likely to be specific to MCs and a more appropriate target for intervention. For example, the CADM1 partners, TIAM in acute T cell leukaemia [49] or PTPN13 in epithelial cells [50], are not expressed in MCs. Also, no link between CADM1 and Mcl-1 has so far been reported. The molecular mechanisms of the CADM1-mediated pro-survival pathway are not known, but by delineating isoform-specific expression and intracellular signalling within different cell types, there is the potential to modulate cell and tissue-specific CADM1-dependent processes favourably.
This work is supported by project Grant no. 87834 from the Medical Research Council, UK (P.B. and M.L.L.) and was conducted in laboratories part-funded by European Research Development Fund #05567. The authors are grateful to Michelle Powel and Dan Deeping for preparation of HLMCs.
Conflict of interest
The authors declare that they have no conflict of interest.
Open Access
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.


text:
forum: Food & Drink
Pages: 1 2 | Home » Food & Drink » One pot Meals
#1 Fri 06 Mar 09 10:38am
young mum
- Forum champ
- Member since Tue 22 Mar 05
One pot Meals
So what is your favourite One Pot Meal?
#2 Fri 06 Mar 09 11:35am
The White Rabbit
- Forum super champ
- From Sydney, Australia
- Member since Tue 22 Jun 04
Re: One pot Meals
risotto but i'm not sure if it's a true one pot meal as you don't tend to add it all at the start
#3 Fri 06 Mar 09 11:41am
- Forum champ Occupation Newbie Mamma
- From Italy-UK-Spain
- Member since Mon 16 Jun 08
Re: One pot Meals
I was wondering about these one pot meals. is this the type of food you make with a "slow cooker" "Croc pot"???
I'm very inetrested, don't know much about this.
#4 Fri 06 Mar 09 12:01pm
- Forum super champ Occupation Just being me
- Member since Fri 28 Mar 08
Re: One pot Meals
I love one-pot meals and have posted many of my favorites in other threads. It's a dish that is complete enough that you can serve it as the whole meal or with a small salad.
This one looked good to me, haven't tried it yet.
Turkey and Black Bean Tamale Pie
by Rick Rodgers
Try substituting hominy for the black beans, or adding a chopped green bell pepper to the saucepan with the onion.
2 tablespoons olive oil
1 medium onion, chopped
1 jalape�o, seeded and minced
2 garlic cloves, minced
3 tablespoons all-purpose flour
2 tablespoons chili powder, or more to taste
2 cups homemade turkey stock or canned reduced-sodium broth
One 8-ounce can tomato sauce
1 teaspoon salt, divided
3 cups cooked turkey, cut into bite-sized pieces (about 12 ounces)
One 15 1/2-
ounce can black beans, drained and rinsed
1 cup thawed frozen corn kernels
1 1/2 cups yellow cornmeal, preferably stone-ground
1 cup shredded extra-sharp cheddar cheese (4 ounces)
1. Position a rack in the center of the oven and preheat the oven to 350�F (175�C). Lightly oil a 9-by-3-inch baking dish.
2. In a medium saucepan, heat the oil over medium heat. Add the onion and jalape�o, and cook, stirring often, until the onion is golden, about 4 minutes. Add the garlic and stir until fragrant, about 1 minute. Sprinkle with the flour and chili powder, and stir until vegetables are coated.
3. Gradually stir in the broth, then the tomato sauce and 1/4 teaspoon of the salt. Bring to a simmer, then reduce the heat to medium-low. Simmer, stirring often to avoid scorching, until the sauce thickens, about 5 minutes. Stir in the turkey, black beans, and corn. Pour into the prepared dish.
4. In another medium saucepan, bring 1 1/2 cups water and the remaining 3/4 teaspoon salt to a boil over high heat. In a small bowl, whisk the cornmeal with 1 1/2 cups cold water. Whisk into the boiling water and cook, whisking constantly, until the mixture is thick and boiling, about 1 minute. Spread over the turkey mixture as smoothly as possible. Sprinkle with the cheese.
5. Bake until the turkey mixture is bubbling, about 30 minutes. Let stand for 5 minutes before serving.
Last edited by MsPablo (Fri 06 Mar 09 12:04pm)
#5 Fri 06 Mar 09 8:24pm
- Forum champ Occupation Secretary
- From Dublin - Ireland
- Member since Sun 01 Apr 07
Re: One pot Meals
Pasta bake! cant go wrong with pasta bake.... and so many combos.... the choice is yours......
Though, I'm partial to casseroles at winter time... a nice meaty one with loads of wine, herbs, garlic and veggies.... Yum!
#6 Fri 06 Mar 09 11:40pm
- Forum super champ Occupation Chief cook and bottle washer
- From Northern California
- Member since Sat 10 Feb 07
Re: One pot Meals
Coc au Vin. Have not made it in years but I love it with a loaf of crusty bread.
#7 Sat 07 Mar 09 7:55pm
- Forum champ Occupation Secretary
- From Dublin - Ireland
- Member since Sun 01 Apr 07
Re: One pot Meals
Oh eddie, you're the man!
Coq au vin....
#8 Sat 07 Mar 09 8:15pm
- Forum super champ Occupation avoiding housework
- From The land of song.
- Member since Tue 04 Oct 05
Re: One pot Meals
Chicken Casserole always goes down well .
#9 Mon 09 Mar 09 5:39am
- Forum champ
- From Sydney, Australia
- Member since Tue 22 Jun 04
Re: One pot Meals
One Pot Potato Soup
4 peeled potatoes
1 small onion
4 green onions
1 garlic clove
3 tbsp butter
3 cups chicken broth
3/4 cup whipping cream
1 cup milk
dash white pepper
1. Chop the potatoes, onions and green onions. Finely chop the garlic clove.
2. In heavy skillet, lightly saute the onions and garlic.
3. Add the potatoes and chicken broth. Simmer until the potatoes are tender.
4. Mash the potatoes, onions and garlic with a potato masher. Add the cream, milk, salt and pepper and heat through.
#10 Mon 09 Mar 09 6:55pm
- Forum champ Occupation NLP Trainer and Coach
- From Portugal
- Member since Tue 09 Dec 08
Re: One pot Meals
Fish Moqueca is one pot meal, also rabbit stew with beans, portuguese fish caldeirada, seafood rice...on and on and on!!!
Tough thing is I luv them all!
Pages: 1 2 | Home » Food & Drink » One pot Meals


text:
Your guide to this month's biggest and best comedy shows and stand-up in London, including Hardeep Singh Kohli, Jimmy Carr, Jenny Eclair and Channel 4's Comedy Gala
Click here to launch the guide to comedy in London this May
Udderbelly Festival
Southbank Centre, until Jul 14
The big purple udders are back at the Southbank Centre and on various dates you'll get the chance to laugh along to the likes of Doc Brown, Gina Yashere, Mark Dolan, Nick Helm, Ian Stone and Susan Calman. There are various sketch shows and cabaret acts on offer too.
Southbank Centre, Belvedere Rd, SE1 8XX, various times, www.underbelly.co.uk
Omid Djalili
Leicester Square Theatre, Apr 30-May 25
Fresh from floundering around like a majestic turkey on ITV's competitive diving show Splash! Omid Djalili shares his usual concoction of daft voices, dress-up and all-round silliness.
Leicester Square Theatre, 6 Leicester St, WC2H 7BX, 4.30pm 7.30pm, £28, www.omidnoagenda.com
Andy Parsons
Rose Theatre, Kingston, Sun May 5
Andy Parsons, the man with TV's fruitiest voice, steps away from Dara O'Briain's protective embrace and out of the studio to embark on a solo tour, named I've Got a Shed.
Rose Theatre, 24-26 High St, KT1 1HL, www.andyparsons.co.uk
Hardeep Singh Kohli
Kings Place, Thu May 9
Singh Kohli's new show, Hardeep is Your Love, examines the pitfalls and triumphs of a pure soul searching for love. Unfortunately that soul is trapped in the body of a 43-year-old, hairy, fat, brown man from Glasgow. The material is based on his endearingly honest Scotland on Sunday column of the same name.
Hall Two, Kings Place, 90 York Way, N1 9AG, 8pm, £12.50, www.hardeeplive.com
Eddie Izzard
Wembley Arena, May 11-12
National treasure Eddie Izzard hits London this month. Expect surreal digressions and fabulous nails.
Wembley Arena, Arena Square, Engineers Way, HA9 OAA, 8pm, £44.75, www.eddieizzard.com
Jack Dee
Hammersmith Apollo, May 11-12
Britain's favourite curmudgeon returns to the road after a six-year absence. He's back on his most cantankerous form in a show dedicated to – what else? – his pet peeves. What's more, the Saturday night show will be recorded, so if you want your own 15 minutes, book front-row seats.
Hammersmith Apollo, 45 Queen Caroline St, W6 9QH, 7pm, £25, www.offthekerb.co.uk/jack-dee
Jenny Eclair
Harrow Arts Centre, Fri May 17
The always acerbic Jenny Eclair makes a flying visit to London this month, delivering her own unique brand of world-weary observations.
Harrow Arts Centre, 171 Uxbridge Road, HA5 4EA, 8pm, £17.50 www.jennyeclair.com
Jimmy Carr
O2 Academy Brixton, Sat May 18
The reigning champion of the cruel one-liner takes his Gagging Order tour to Brixton. Filthy, immoral and utterly unrepentant, this is a show for people who prefer their laughs on the less politically correct end of the comedy spectrum.
O2 Academy Brixton, 211 Stockwell Road, SW9 9SL, 7.00pm, £28.12, www.jimmycarr.com
Channel 4's Comedy Gala
O2 Arena, Sun May 20
The big kahuna of May comedy is this magnificent array of famous faces. It's a huge show in aid of a good cause: Great Ormond Street Hospital Children's Charity. The biggest names in comedy will all take the stage, including Alan Carr, Jack Dee, Jo Brand, Jonathan Ross, Lee Evans, Michael McIntyre, and Noel Fielding.
O2 Arena, Millennium Way, SE10 0DX, 6.30pm, £40-£100, www.channel4.com
Micky Flanagan
Hackney Empire, May 30-31
The geezer's geezer takes time out from his punishing schedule of TV panel shows to play two triumphant dates on his home turf, east London. Expect charmingly irreverent jokes about class and social mobility.
Hackney Empire, 291 Mare Street, E8 1EJ, £28.50, www.mickyflanagan.com
- More about:
- Arts
- Kingston Upon Thames
- O2 Arena
- Performing Arts
- Stand-Up
- Theatre


text:
Macks Prairie Wings EST 1944 Spirit Football Jersey is the perfect shirt for the fall. This MPW spirited oversized shirt has football style sleeves for extra room and comfort. Sometimes bigger is better and this is true with the extra large MACK'S lettering from shoulder to shoulder. The drop hem on the sleeve gives you that sporty style. Support your favorite waterfowl outfitter.Cool, comfortable cotton material. Machine wash and dry.
Mack's Prairie Wings is not responsible for typographical errors in pricing or product specification inaccuracies in our web site. Prices are subject to change without notice.
Copyright © 2017 - Mack's Sport Shop LLLP - All rights reserved. Site Developed and Maintained by Ability Commerce
Copyright © 2004-2017 - Mack's Prairie Wings, Inc. - All rights reserved. Site Developed and Maintained by Ability Commerce


text: Alight guys need some help. I am currently running DC mud country 33s with stock 17" rims on my '05 1500 Z71 w/ front end leveled (via torsion bars/no trimming required & minor occasional rubbing during full sharp turns). I have decided to upgrade w/ a 4" Rancho lift along w/ some new trail grapplers and 18" rockstar wheels (18x9 w/ 0 offset). Question #1-What is the biggest tire I could run w/ this setup ? (I know 35s easily but wasn't for sure on 37s or not). Question #2- W/ the rim size & offset, would this setup cause my tires to stick outside the fender flares? Not going for the wide, knobby look and wanting to stay near flush to the flares. Thanks for all the post/advice! Take care & God bless.


text:
Sat, Aug 10, 2013 at 10:56 AM
Comment War-
so i decided 2crown mislf Queen of Comments- & of course some1 wanted 2challenge me..and thy lost!! if u thnk u gt wat it tkes u shuld challenge me. the commnts r mail can b abt anythng_ but understnd i will gt the last word-


text:
Diamond Trellis 180x30cm from Woodlands
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Our Price £10.99
- Your delivery address must be in our local delivery area.
- Order for collection at Bagshot, Bybrook Barn or Shepperton.
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Click here for more information on returning this product
Product DetailsConstructed from planed and pressure treated European softwood with mortice and tenon jointed frames.
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text:
Joe Lhota, The MTA's Rider/Chairman, Unanimously Confirmed
After sailing through two committee hearings, Joseph Lhota was unanimously confirmed by the New York State Senate to be chairman and CEO of the Metropolitan Transportation Authority on Monday.
"I'm very pleased and honored," Lhota said afterwards, speaking to reporters outside the Senate gallery. "I'm looking forward to this opportunity to make a difference."
But it was the state senators themselves who sounded humbled.
"We're honored Joe would come back to public service," said state Senator Malcolm Smith, who seemed to be speaking for most of his colleagues. Lhota was a former New York City deputy mayor in the Giuliani administration, and had been an executive vice president at Madison Square Garden when New York Governor Andrew Cuomo tapped him for the MTA to replace Jay Walder.
Many senators expressed astonishment that the new MTA chair would want the job. The MTA is largely viewed by legislators as "insular, inefficient, and — dare I say it — arrogant," state Senator Andrew Lanza said. Senator Charles Fuschillo, chairman of the senate's transportation committee, summed it up: "We've heard this is the most bloated bureaucracy in the country, we've heard about the double books, we've heard about every problem – we've even seen people on the front page of the (New York) Post the other day, playing chess when they should be working."
Lhota, for his part, was even tempered throughout, although he did use his time on the hot seat to to impart a couple of teaching moments. After hearing several legislators trot out the old trope that the agency has two sets of books -- a misperception that is almost a decade old -- he bristled. "The fact of the matter is when you go to our website and drill down, you'll see an enormous amount of information," he said, adding that the MTA is one of the most transparent agencies in New York State. "There never was two sets of books," he said, "and there never will be two sets of books."
But the senators knew they had a political macher, not a transportation wonk, in their court, and they seemed to be going through the motions. While Lhota fielded questions on everything from the MTA's finances, to overtime pay, to his stance on tolling the East River bridges, the only legislators who seemed able to muster genuine indignation were the perpetually offended Ruben Diaz, who was unhappy about a subway station in his district, and representatives from Dutchess and Orange Counties -- two of the four so-called "quarter pounder" counties, who share one vote on the MTA board and feel overcharged and underserved by Metro-North.
When senators opined wistfully about the possibility of cutting back on taxpayer support, Lhota sought to nip that in the bud. "I do have to bring up one thing, and I'll be very honest and very blunt," he said. "There is no way that the MTA can operate without taxpayer money. It was never envisioned to be run nwithout taxpayer dollars. There is not a transportation or commuter rail or transit system in the country that doesn't work without some other infusion of cash...The entire operation of the MTA cannot be paid for from the riders. It was never envisioned that way when the legislature created the MTA in 1968. I just want to be able to say that."
During the hearings he talked about his vision for the MTA -- one in which the already pared-down agency further streamlines while improving service. Lhota said he'd be looking closely at the agency's back office operations. "Each one of the operating agencies of the MTA (has) an enormous amount of redundancies," he said. "They all have their own legal staff…. All of the administrative functions are duplicated. I think the time has come for there to be one MTA."
And he said he was realistic about the challenge: "The bottom line is there's no consistent standard of excellence across all the MTA. In most cases the service is reliable, stations are clean, and employees provide good customer service. But we've all seen dirty subways, we've all seen elevators and escalators out of service, buses that crawl at four miles per hour, commuter rail service crippled by bad weather, we've heard about projects over budget and behind schedule."
By the end of the afternoon, when the full senate convened to vote, legislators buoyed by the promise of a new era at the MTA rose to their feet to give the new chairman a standing ovation.Lhota told reporters afterwards that he's ridden the subways all his life, and he'll continue to do so. (But with a power that few straphangers can exercise.) "As a rider, I'm also going to be a critic, when I see something wrong on the subways I'm going to make sure it gets fixed," he said. "So the type of chairman I'm going to be for riders? I'm going to be a rider/chairman."


text: I have a stealth II programmer but I need the DPF delete files before I can install it. Does anyone know where I can download these files? The company has shut down the website with all the downloads and now I'm stuck. I tried to search the forums but I'm in a bit of a rush on this. thanks everyone.


text:
University Compliance
Academic Health Care Compliance
Academic Health Care Compliance (Academic Programs Related to Health)
University Compliance Network Contact:
Warren Olson, Health Compliance Officer, x15867
Representative Compliance Areas:
Academic programs related to health care professions.
Helpful Resources:
The Health Compliance website provides information for "students, faculty, and staff participating in clinical or non-clinical experiential education or research at health-related affiliated facilities." It includes links to requirements, affiliation agreements, training and events information, and contact information for Health Compliance staff.
Page last modified May 23, 2016


text:
Welcome from Utah.
This is a discussion on New from Georgia within the New Members Introduce Yourself forums, part of the Welcome To DefensiveCarry.com category; Welcome!...
Duty, Honor, Country...MEDIC!!!
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Welcome from Utah.
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Retired DE Trooper, SA XD40 SC, S&W 2" Airweight
dukalmighty & Pure Kustom Black Ops Pro "Trooper" Holsters, DE CCDW and LEOSA Permits, Vietnam Vet 68-69 Pleiku
Enjoy the forum....................
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The situation will NEVER BE THE WAY YOU WANT, it WILL BE THE WAY IT IS. You must be FLEXIBLE ENOUGH TO ADAPT and just "DEAL WITH IT".
Welcome to Defensive Carry from Olympia, WA; in the Great Pacific Northwest!
Only two defining forces have ever died for you.....
1. Jesus Christ.
2. The American Soldier, Sailor, Airman, Marine, and Coast Guardsman.
One died for your soul, the other for your freedom!
Welcome aboard from the great state of Pennsylvania!


text:
Two charged after early morning shootout
Started as a chase on Interstate 81
GRAY, Tenn. - UPDATE 12:45 p.m.: State troopers have given us more information about the suspects involved with the chase and shooting. View mugshots and photos from the scene
We're told Derrick Kitzmiller, 20 years old, from Gray, Tennessee, was driving the vehicle. He is charged with attempted second degree murder, driving on a suspended license, felony evading, reckless endangerment and speeding.
The passenger and owner of the car, 35-year-old Jennifer Russell of Bristol, Tennessee, is charged with felony altering of a title or registration.
UPDATE (8:55 A.M.): News 5 WCYB has learned more than 14 shots were fired between a Tennessee Highway Patrol Trooper and a pursuit suspect this morning along Hog Hollow Road in Washington County, Tennessee. View photos from the scene
The case started as a tailgating complaint along Interstate 81 northbound.
We've learned dispatchers received a call at 2:14 a.m. from a person saying they were being tailgated along the Interstate.
Troopers say a pursuit began around Exit 50 norhtbound on Interstate 81 and the chase continued onto Interstate 26 eastbound. We're told the suspect then exited onto Eastern Star Road and then turned on to Hog Hollow Road.
We're told the suspect's car then crashed along Hog Hollow Road.
According to troopers on the scene, a shootout followed between the suspect and Trooper Jeff Applebea.
We're told there were at least 14 shots fired between the trooper and the suspect. Troopers say no one was injured.
Two suspects were arrested and taken to the Washington County Detention Center.
There is no word on the connection between the suspect and the initial caller in the tailgating complaint.
Hog Hollow Road remains closed. News 5 WCYB's Meredith Machen is on the scene and will have more facts throughout morning online and on News 5 At Noon.
Most Popular
- Kidnapping suspect still on the run, after statewide AMBER Alert is canceled
- Lightning strikes BVU sub station in North Bristol, 3,700 customers without power
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- Foreign suspect sends bomb threat by fax to Meadowview Center in Kingsport


text:
Herdim Precision Rasp Half-Round, Slim, Cut 5
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text:
Against the backdrop of the most digital Christmas ever a new study from technology research firm EKN shows that most retailers don't believe they are spending enough on the Internet, mobile, business intelligence and analytics.
EKN, with sponsorship from Cisco, Earthlink and Tata Consultancy Services, surveyed more than 120 retail information technology executives and uncovered some disturbing findings given the direction in which consumers are leading the retail industry.
For example, although technology is viewed as a strategic enabler for customer-centric retailing, driven by the need for deeper customer insights, omnichannel integration, enterprise mobility and a host of other priorities, the current retail IT cost structure isn't aligned with these strategic priorities. That puts many retailers at a competitive disadvantage, according to EKN.
"Technology must become a strategic enabler of new-age retail, and this report is the first to provide an honest, open assessment of the true state of retail IT," said Gaurav Pant, research director, EKN. "The good news is that retailers can take steps to improve their strategic alignment and bring IT's priorities in line with the true needs of the business."
EKN's research also found that while retailers acknowledge the importance of technology in building the capabilities to thrive in a consumer-driven retail environment, 70% of retailers consider themselves at par or behind competitors in terms of strategic use of IT, something EKN viewed as a telling indictment of the inability of retailers to leverage IT to drive competitive differentiation.
Other insights from the report titled, "The New Cost Structure of Retail IT," included the following:
- Customer engagement is not IT's top priority. For all of IT's supposed strategic relevance to driving customer engagement, retailers consider technology as primarily a tool to improve operational efficiency. Retailers that use IT as a way to differentiate themselves from the competition are more the exception than the norm.
- IT is not seen as a strategic business unit. The organizational alignment is off-center, forcing IT to be driven more by cost-reduction than by strategic business growth. The fact that retail leadership still considers IT a cost-center is reflected in its leadership alignment. According to EKN's research, in 2013 45% of CIOs reported to the CFO or COO, while 65% of CMOs reported to the CEO or the Board. Shifting this mentality will not be easy, especially as few retailers possess the ability to measure investments in IT against business outcome.
- IT budgets are constricted. Over the past decade, 1.5% of revenue has been the industry benchmark for IT spending, and budgets will remain flat in the coming year. However, retailers are spending more on technology directly through business functions such as marketing and e-commerce. And, this trend is expected to continue to drive IT spending in retail.
- Retailers are under-spending on Web, mobile, BI and analytics. Fifty percent of retailers state they are under-spending on Web and mobile. With 30% of brick and mortar retailers' revenue expected to come from web and mobile by 2016, this points to a strong disconnect between business demands and current IT spending. The need for deeper customer insights to drive stronger customer relationships is near or at the top of every retailer survey that EKN has undertaken in the past year; yet, 45% of retailers state they spend too little on business intelligence and analytics.


text:
Home › Forums › Gear Forums › Gear (General) › Packs @REI recommendations
Feb 24, 2013 at 8:21 am #1299647
Hi Folks,
As I live in the middle of nowhere the nearest outdoor shop is an REI 2 hours away, I will be in the vicinity next weekend
And was going to try some packs.
It would be for winter/shoulder season use so something around 60 litres and capable of carrying 45lbs or so.
I undertstand I need to get a pack that fits me properly but I looking for some recommendations of what folks have bought from REI in the past or are famillar with what they sell.
StephenFeb 24, 2013 at 8:42 am #1958058
Charles PSpectator
@mediaurasLocale: Terra
The Osprey Aether 60 is a nice pack. Not UL, but it carries weight well.Feb 24, 2013 at 9:05 am #1958073
Nathan WattsBPL Member
I'll second the recommendation for the Osprey brand. Used them in the past and they're great packsFeb 24, 2013 at 9:21 am #1958076
Thanks Guys,Feb 24, 2013 at 9:29 am #1958079
Konrad .BPL Member
Stephen, some of the REIs carry granite gear. You'll likely find them to be the most light weight packs that can carry 40-50 lbs at a rei. Look for the framed granite gears (e.g. Nimbus, snow leopard, etc)Feb 24, 2013 at 9:32 am #1958080
Travis LeannaBPL Member
@t-lLocale: Wisconsin
Third for osprey.Feb 24, 2013 at 9:51 am #1958089
Phillip AsbyBPL Member
@pgasbyLocale: North Carolina
I have an REI Flash 65 I like a lot (current model is the 62 I believe)… Worth trying I think!Feb 24, 2013 at 9:52 am #1958091
Thanks for the recommendations.Feb 24, 2013 at 10:16 am #1958098
Max DiltheySpectator
@mdiltheyLocale: MaxTheCyclist.com
Ospreys are great. Guaranteed to enjoy it.
Other brands to look for that are sometimes regionally held in REI stores that I like:
Boreas (I love mine!)
The REI Flash packs
I haven't had good luck with Deuter, and Kelty bags are very heavy but I still like my Kelty.
Let us know what you choose!Feb 24, 2013 at 10:53 am #1958121
Ben 2 WorldBPL Member
@ben2worldLocale: So Cal
Yeah, Gregory, Granite Gear, Osprey. I find then more expensive, but better than REI's own brand backpacks. YMMV, of course.Feb 24, 2013 at 10:56 am #1958124
I will check out the Boreas packs also.Feb 24, 2013 at 11:06 am #1958128
Will do Max.Feb 24, 2013 at 1:16 pm #1958185
HkNewmanBPL Member
@hknewmanLocale: Western US
Those Granite Gear Leopards look interesting if one googles images to how the crampon patch actually works (it's a crampon patch that attaches to those multiple belts … mini-MOLLE attachments it seems … on the back). Looks like the ladies are getting the good colors on this series.Feb 24, 2013 at 1:24 pm #1958193
Ben 2 WorldBPL Member
@ben2worldLocale: So Cal
One more thought… given a 4-hour drive round trip — you will want to be sure that the store carries the packs you're interested in — and in your size. Two weeks before your pilgrimage… call ahead and run through your list. Anything that you are particularly curious about but they don't have on hand — order them online — to be delivered to the store. Yes, they will charge your card — but free shipping — and it's an easy way to ensure that ALL the packs you are interested in will be there for you to try out. And whatever you don't wish to buy — simply return them right at the store. REI will still be able to sell those packs as new — so no harm done to anyone.
EDIT: Just realized you're going next week. But anyway, food for thought when planning for future visits.Feb 24, 2013 at 1:27 pm #1958197
Cheers Ben,
That's a good idea, I was going to be in the area at concert next Saturday so was going to dop in before hand.Feb 24, 2013 at 1:28 pm #1958198
Travis LeannaBPL Member
@t-lLocale: Wisconsin
Stephen, since you're headed there anyways, I have a list of things I'd like….Feb 24, 2013 at 1:30 pm #1958201
Fire away Travis I can bring them to the GGG.Feb 24, 2013 at 1:57 pm #1958213
External frame?
I love my deuter act lite(internal frame), and it was the most comfortable with 35 lbs of all the packs listed so far. I literally spent all day trying on every pack REI carries.
That being said, I'm not sure that I would look forward to carrying 45lbs in it all day.
I recently picked up an old Jansport external frame, and after removing the top cross bar, and the "shelf" bar, both unnecessary. It came in at 3lbs 5oz. Lighter than my Deuter, and feels like it would carry the load better. I'm going to use it on my next trip to find out. At the very least it will get rid of my sweaty back!
Oh, and it only cost me $20!
You might be able to cut even more weight if you get rid of the pack bag and just strap stuff sacks to the frame. Be carefull that all the stuff sacks and straps don't add up to more than the original pack bag.Feb 24, 2013 at 2:01 pm #1958215
Hi Nick,
It would be an internal frame I am looking for.
CheersFeb 24, 2013 at 2:10 pm #1958222
Luke SchmidtSpectator
@cameronLocale: Idaho Falls
REI has some good packs but they can get pricey, especially if you go with a brand like Gregory. Check them out but don't rule out options like the HMG Expedition that are not carried by REI. Higher end REI packs aren't really much cheaper then cottage companies like HMG or even a stripped down McHale pack.Feb 24, 2013 at 2:20 pm #1958227
Hi Luke,
I would rather buy a pack in store and also REI will let me bring it back if it doesn't work out a I have had some with a Ula pack on my last trip.
Cheers,Feb 24, 2013 at 3:03 pm #1958247
"Hi Nick,
It would be an internal frame I am looking for.
In that case check out the Deuter ACT lite. Very comfy pack, but YMMV.
The only thing I don't like is the sweaty back, but all of the vented back packs had bad hotspots at the time. Designs may have changed in the last couple of years though.Feb 24, 2013 at 3:12 pm #1958252
I will keep an eye out for that Nick.
When you say hotspots do you mean sore spots?Feb 24, 2013 at 4:42 pm #1958289
Yeah. After a half hour of walking around I started to notice pressure on my lower back, like the arched frame was digging inFeb 24, 2013 at 4:55 pm #1958294
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