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Take Account Opening. Please.
Posted by Site Administrator on March 26, 2007
- 308 Recommendations
- 0 comments
by Craig Le Clair.
Often when I order takeout food, the restaurants know who I am right away — as if I was a member of the family. At some restaurants, my son placed the order regularly in the past and they will ask about him, "Is Justin okay? Why isn't he placing the order today?" They will leverage the information in my record to make the order simpler. "So you take the plain nan with that?"
If these small establishments with limited resources have this kind of personalized service, why is it that the biggest financial institutions in the world seem to not have any idea who I am? And if I want to extend my business with them, why do I need to start from scratch?
Information managers I speak with struggle to address these pain points in external communications with customers, clients, citizens, agents, and stores; account opening is near the top of the list of pain points. It exists at the lonely edge of the business process where the company interacts with the client — where information bounces between a company's firewall and the customer's domain — but why is this so difficult?
For one, business must deal with more diverse use cases. These relate to the multi-channel environment and the diversity of capture options that now exist. The paper world, despite inefficiency, had one primary use case with a small number of potential variations. Everyone — customers and operations — were trained in the simple paper model.
Today firms are dealing with the Web, email, paper mail, faxes and more with each of these communication channels which frequently overlap despite different underlying technologies. In short, the number and diversity of use cases have grown immensely including the complexity of the back-end workflow.
And think of how bad it can be. As a potential customer, you are persuaded to take action. That exciting TransPromo document with the enticing one-time offer on your phone bill may have swayed you, or perhaps it was an email promotion that did the trick. So you search for and find the correct form and fill it out. Then you fax or mail it in and with any luck it ends up at right location. Further good fortune turns the paper or fax into an electronic fil and forwards it to an indexing queue. Here off-shore staff extracts the needed data and enters it into some transaction system. But corrections are needed that require circling back to the customer. It is re-processed with corrected information and finally a confirmation package is sent that of course requires a "wet signature". So, a second capture process is needed and final documentation is sent out.
There are lots of points where errors can be introduced — lots of systems that must interact successfully. And today there are 100's of client-facing business processes that need to be improved.
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subset: commoncrawl


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About his wing span,
Nick's "freakish" wingspan compared to top NFL receivers
BSIMatt : 4/29/2009 4:29 pm
The article basically goes on and compares Michael Crabtrees wingspan to some big name NFL receivers like Calvin Johnson, Braylon Edwards, Brandon Marchall, Vincent Jackson, etc, to point out that despite his height, he actually has a longer wingspan than every WR mentioned.
Went back and looked at Nicks wingspan, and he in fact, at 33.5 would put him ahead of those very same receivers and only behind Crabtree.
Nicks is a gamer and will be taken care of sooner rather than later by the Giants.
As I said, he's not small at all, but he's not especially big either. Its really no big deal.
"Typical Morehead!"..................and he didn't even mean that as a compliment..


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Maliyadeva Girls College, Kurunegala (Girls Championship) and Vidyartha College, Kandy 'B' (boys championship) won the under 19 'A' Division titles at the all island schools Table Tennis Championship organised by the Sri Lanka Schools Table Tennis Association and worked off at the Indoor Stadium of St Thomas College, Mount Lavinia.
Maliyadeva Girls College which has produced top paddlers in the past did extremely well in the girls under 19 finals to defeat Southlands College, Galle 3/1. Champion girls team had in its fold Pawani Sirisena, Buddhipani Rajaguru, Rashini Rajapakse, Isuru Godawita and Dinushi Upamali. It must be mentioned that Buddhipani Rajaguru was in extremely good form last year coming up with good performances which she has carried forward this year and this being the first Table Tennis Tournament for the year by the SLSTTA.
Vidyartha College maintained their reputation as the best school in Table Tennis by their 'B' team winning the boys under 19 'A' Division title. They have so much talent in the school that it was Vidyartha College's A and B team which figured in this final with the 'B' team carrying away the championship honours. Fielding two teams and dilution of talent did not stop Vidyartha carrying away both the champion and runners up titles.
Under 19 girls 'B' Division: G/Prajapathi Gothami Balika Vidyalaya, Ambalangoda beat Southlands College, Galle 3/0.
Under 19 girls 'C' Division: Holy Family Convent, Kalutara beat Ladies College, Colombo 3/2.
Under 19 girls 'D' Division: Holy Cross College, Gampaha beat Royal Institute International School, Nugegoda 3/2.
Under 19 girls 'E' Division Hillwood College, Kandy, beat Loyola College, Bopitiya 3/2.
Girls Under 14 Championship
Dharmasoka College, Ambalangoda another school which has stamped its class in Table Tennis clinched Girls under 14 team championship by defeating Girls High School, Mount Lavinia 3/1 in the finals. Dharmasoka College was powered by top players like Imasha de Silva, Kavindhya Sheshadi, Hasini Sandipani, Viduni Odeesha and Sathya Udayangani. Mount Lavinia Girls High School were represented by Chamathsara Fernando, Ashani Atappatu, Yahari Mendis.
The results of the other 14 girls team championship were:
Girls under 14 'B' Division Maliyadewa Girls College, Kurunegala beat Holy Family Convent, Kalutara 3/2.
Girls Under 14 'C' Division: Anula Vidyalaya, Nugegoda beat Ladies College, Colombo 3/1.
Girls Under 14 'D' Division: Ladies College, Colombo beat Holy Cross College, Gampaha 3/2.
Girls Under 14 'E' Division: St Anthony's Girls College, Kandy, beat Anula Vidyalaya, Nugegoda 3/2.
Girls Under 14 'F' Division: Hillwood College, Kandy beat Dudley Senanayake Central College, Tholangamuwa 3/2.
Girls Under 14 'G' Division: St Mary's Convent, Matara beat Karandeniya Central College, Karandeniya 3/0.
Results of the boys under 19 team championship were,
Boys Under 19 'B' Division: Maliyadewa College, Kurunegala beat Royal College, Colombo 3/0.
Boys Under 19 'C' Division Royal College, Colombo beat Wesley College, Colombo 3/1.
Boys Under 19 'D' Division: Dudley Senanayake Central College, Tholangamuwa beat De Mazenod College, Kandana 3/1.
Under 19 Boys 'E' Division: St Peters College, Colombo beat Ananda College, Colombo 3/1.
Under 14 Boys Team Championship
Dharmasoka College, Ambalangoda clinched the double in the under 14 team championship by winning the team championship for boys as well by defeating Vidyartha College 3/2 in the 'A' Division finals. Dharmasoka College earlier won the under 14 team championship for girls.
Boys Under 14 'B' Division: Maliyadeva College, Kurunegala beat Vidyartha College, Kandy 3/0.
Boys Under 14 'C' Division: Kegalu Vidyalaya beat Vidyartha College 3/1.
Boys Under 14 'D' Division: Sumangala College, Panadura beat Dudley Senanayake Central College, Tholangamuwa 3/2.
Boys Under 14 'E' Division: Gangasiripura Maha Vidyalaya, Gampola beat Ananda College 3/2.
Boys Under 14 'F' Division: Dudley Senanayake Central College Tholangamuwa beat St Peters College, Colombo 3/2.
Boys Under 14 'G' Division: Warapitiya Maha Vidyalaya beat Dharmaloka Vidyalaya, Kelaniya 3/1.
Boys Under 14 'H' Division: Lyceum International School, Wattala beat Loyola College, Bopitiya.
S M Jiffrey Abdeen - Kandy Sports Corr -Courtesy Daily News
Should Dilshan have continued until Cricket World Cup 2019?
"It was quite an embarrassing defeat. It was pure poor batting and the execution. You have to be really skillful to bat against these guys, especially in these conditions. Anderson once again showed his class. Stuart Broad was brilliant as well. One or two of our guys needed to raise our hands and put up a good score, particularly in the first innings. We failed to do that in the first innings, and once again in the second innings, so that's a pretty obvious reason for our defeat." - Angelo Mathews
Cricket is a game of fine margins and Mathews is set to be a modern great. Hang in their Angie, this team can achieve something on this tour. Thirimane and the youngsters form was a real positive. Prasad was missed. Chameera should be persisted with. If Bairstow was our early outcome could have been very different.
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Date of Stay: May, 2010
The camp website indicates that it allows dogs, but it is not pet-friendly at all. While trying to obey the many pet rules, and given that it was our first (and last) visit, we were continually given a hard time and made to feel very unwelcome. Also, a staff member was VERY RUDE to one or our guests. While the site was spacious, and the park clean, I would never recommend this campground to anyone that expects to be treaty respectfully and fairly.
Date of Stay: August, 2009
We had a lovely visit. The pool area was fun, the lake was clean and inviting. Lots of activities for children. The only down side is that it is very crowded. But everyone was very friendly and the dog park was an added bonus.


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HST Emergency Coupling
Saturday Cinema - Transport Weekend (again) *If* you are driving your HST (High Speed Train) and have to couple up to another train this video is just for you. Invaluable information for the aspiri
By: thecleaner001 | Comments: 13 | Views: 1788 | Votes: 7 | Shared: 0
Location: United Kingdom (UK/GB) | Leaked: Sep-3-2016 in Yawn, Vehicles, History
Re-railing a HST back on the road..
An HST (High Speed Train) has come off the road (Power Car No 43035) and the Breakdown Crew from Plymouth (Lara) attend to do the business. (birds whistling and church bells ringing in this Cornwalli
By: thecleaner001 | Comments: 27 | Views: 2673 | Votes: 21 | Shared: 4
Location: England, United Kingdom (UK/GB) | Leaked: Feb-13-2016 in Other Entertainment, Vehicles
LiveLeak Podcast Thursday 13 August 2015 1030 Hours HST
All of Troll Centrals Comments... Have a Laugh! mrgod2u: mrgod2u: I love liverwurst!(2:14pm 6th Aug)....(10:28am) devries: hello(10:28am) devries: am I 4th?(10:28am) mrgod2u: LOL's t
By: mrgod2u | Comments: 21 | Views: 93018 | Votes: 4 | Shared: 6
Leaked: Aug-13-2015 in Other Items from Liveleakers
Robby Gordon Flies Down A Sand Dune - Dakar 2014 Rally
American Robby Gordon demonstrates his driving skills as he speeds down the Atacama Dunes in his HST Gordini during the Dakar 2014 Rally in Chile. Gordon is competing in his 10th Dakar Rally.
By: hobonickel | Comments: 22 | Views: 2697 | Votes: 1 | Shared: 0
Location: Chile | Leaked: Jan-16-2014 in Other Entertainment, Sports, Vehicles
hst_ gee_ roll by
This guys name is gee he s a freind of mine....he comes from a essex based group called hst ...this is one of his tunes called roll by .....he asked me to promote his hardbodytv channel so please subs
By: bigs31uk | Comments: 4 | Views: 271 | Votes: 0 | Shared: 0
Location: England, United Kingdom (UK/GB) | Leaked: Feb-27-2013 in Music
hst_ gee_ good times
This guy is absolutly smashing it bigtime...check out his other single called good times...excellent track...please subscribe to hardbodytv if you like his music....peace.....bigs
By: bigs31uk | Comments: 0 | Views: 330 | Votes: 0 | Shared: 0
Leaked: Feb-27-2013 in Music
Trains in the snow
trains and locomotives look good in snow and ice... Clay Cross Junction, Derbyshire Jan 2 1997 credit to original film-maker Kevin Smith
By: thecleaner001 | Comments: 9 | Views: 2637 | Votes: 3 | Shared: 0
Location: Nottingham, England, United Kingdom (UK/GB) | Leaked: Mar-1-2012 in Other
InterCity 225 Running to time P2
Pt 2 of the Intercity engineering story
By: thecleaner001 | Comments: 1 | Views: 1292 | Votes: 0 | Shared: 0
Location: Tottenham, Greater London, United Kingdom (UK/GB) | Leaked: Nov-27-2011 in Other Entertainment
Red Nose Train
Japanese High Speed Electric Train
By: thecleaner001 | Comments: 31 | Views: 3672 | Votes: 1 | Shared: 0
Location: Tokyo, Japan | Leaked: Nov-22-2011 in Other Entertainment
The Hubble Ultra Deep Field in 3D
I've recently discovered an animation that was rendered using the measured redshift of all 10,000 galaxies in the Hubble Ultra Deep Field image. Listen to a short script that leads you through a qu
By: wet501 | Comments: 14 | Views: 6564 | Votes: 5 | Shared: 1
Leaked: Aug-13-2009 in Arts and Entertainment
Atlantis visits Hubble: Day two, chatting with the crew
NASA - The stories of the STS-125 astronauts, what they're doing at the moment and how they feel.
By: komakazzi | Comments: 5 | Views: 7871 | Votes: 2 | Shared: 0
Location: Cape Canaveral, Florida, United States | Leaked: May-16-2009 in Arts and Entertainment, Other, Arts and Entertainment
The Uncomprehensible Universe
A beautiful collection of images from around the universe. Some brought to us well before man took his first step on this planet. Others predate the formation of our solar system as we know it. Tru
By: tkdrspm11 | Comments: 18 | Views: 7685 | Votes: 6 | Shared: 1
Leaked: Nov-8-2007 in Other


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Woohoo, here are some super hot new printables to grab. Lots of deals to snag with these so be sure to grab them now! $1.00 off Centrum Silver Multivitamin $1.00 off Centrum Flavor Burst™ Multivitamin $0.50 off any Pledge Multi Surface product $1.50 off any 2 Pledge Furniture Care products $0.75 off 2 Windex […]
Posts Tagged 'Centrum Silver'
Hot New Printables!! Scrubbing Bubbles, Centrum, Windex And More Woohoo!!
September 24th, 2012 vetta
$2.99 Centrum Silver @ Target!
November 29th, 2011 vetta
Take at look at this great deal on vitamin at Target. There is a $5GC when you buy 2, the silver is so far the cheapest ones. Buy two Centrum Adult Silver $9.49 each 140 ct Use (2) – $2/1 Centrum coupons HERE Or use – $2/1 Centrum Adult Silver Women's or Silver Men's 10/09 […]


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Double-Walled Boston Cocktail Shaker
- Summary
Essential equipment for the well-appointed bar, our Boston Shaker is the same two-piece shaker preferred by professional bartenders. Consisting of a pint-sized glass tumbler topped by a stainless-steel shaker, it's considered the best barware for mixing classic cocktails like a Manhattan, Cosmopolitan or Sidecar.
- Easy-to-use two-piece shaker makes mixing perfect cocktails absolutely foolproof.
- Start by measuring ingredients into the glass tumbler, then cover with the insulated stainless-steel shaker and shake until the metal feels very cold to the touch.
- Double-wall insulation keeps condensation from forming on the metal shaker.
- Glass tumbler designed to fit inside metal shaker for compact storage.
- A tight seal guarantees leakproof operation.
- Top-quality cocktail bar essential provides years of dependable service.
- Dishwasher safe.
- A Williams Sonoma exclusive.
- Dimensions & More Info
- Stainless-steel and glass construction.
- 16-oz. cap. 6 3/4" high.
- Use & Care
- Dishwasher safe.
- Shipping
Shipping Information
This item is shipped from our warehouse by UPS to arrive within five business days of our receiving your order.
Shipping Rates
The shipping rate varies depending on your order total and shipping destination. View Shipping Options and Charges.
Next Day Delivery
For an additional charge of $17.50 per address, we can expedite delivery to most destinations. This service is not available for some oversize items, items shipped directly from the supplier (including perishable and custom-manufactured items), out-of-stock items and items to be shipped outside the contiguous 48 states.
Orders reciived by 9:00 p.m., Eastern time, Monday through Thursday will arrive the next business day; orders placed Friday thorugh Sunday will arrive on the following Tuesday.
Return Policy
At Williams Sonoma, we take great pride in the quality and craftsmanship of our products. Attention to design, materials, safety and construction are our priority. Upon receipt, please inspect your purchase and notify us of any damage; we will arrange for a prompt replacement.
If within 30 days, you are dissatisfied for any reason, you may return your purchase for a refund of the merchandise value. An original receipt or gift receipt is required for all returns and exchanges. Returns with a gift receipt will be refunded in the form of a Merchandise Credit for the amount indicated on the gift receipt. Returns with original receipt will be refunded in the original form of payment, cash and check refunds over $100 will be issued as a company check (may take 14 business days from time of request).
For all cutlery returns (knife sets, single knives, cutting boards, knife sharpeners, and knife storage) please contact us at 888.922.4108 to initiate the return.
We cannot accept returns on monogrammed, personalized, special-order items, custom upholstery, food, items shipped direct from the vendor, or on items damaged through normal wear and tear. Final sale items ending in .97 or .99 cannot be returned.
For returns of items purchased from your Williams Sonoma Gift Registry, we will gladly provide a refund or exchange for the merchandise within 90 days of your event or within 90 days of purchase, whichever date is later. View Full Returns Policy.
Rated 2 out of 5 by Dannn from I was happy I picked this up in store and when I got home there was a dent on the bottom of the metal cup, which was not enough of a problem to warrant me driving back and getting another one. I have found that as long you are careful putting the two pieces together it will seal fine, and I was happy with how it performed. Now less than a year after purchasing it, and infrequent use the black lines and numbers are coming off the glass cup. If the glass came with out graduations on the side that would be fine, but since they were there the usability and aesthetic appeal are now greatly reduced.Date published: 2016-07-26Rated 1 out of 5 by projectasia from FAIL - do not buy in stock with 3 day shipping. It took over 10 days to arrive. But happy when it arrived. I washed it with soap and water, all the lettering came off the glass portion (ounces and measure lines). just dish soap and cool water. Ok so i use my shot glass to measure. Put together and the seal rolls over the edge of the glass and cannot seal properly. FAIL, drinks leaked everywhere. I tried multiple times with water, and it does not work at all. see my picture after 1/2 use. Just washed and added some water to shake. It is garbage, and poor quality. Do not buy it unless you plan on not washing or using it. WS, please add a 0 star option, i cannot be more unhappy.Date published: 2016-01-06Rated 5 out of 5 by Jodene from Beautiful! I purchased this for my husband for a birthday present. The quality is excellent! Absolutely beautiful and the monogramming was spot on.Date published: 2015-10-23Rated 5 out of 5 by Sarah1216 from Great Shaker! I love this shaker for several reasons. It's sturdy design makes it easy to use, shake and pour, the double wall keeps contents cold and ice will last longer than in a conventional single wall shaker. The measurements on the glass are convenient. And the rubber ring seals well for shaking. Yes, you have to be careful to position it firmly, and shake with the glass at the top, but it's not a big deal to do so once you get used to it.Date published: 2015-05-26Rated 1 out of 5 by GeneM from Doesn't seal easily and leaks I tried multiple ways to figure out how to shake this without leaking. It can be done but it's tedious and not repeatable.Date published: 2013-12-21Rated 5 out of 5 by DumbClub from Seals Perfectly I have used this shaker for two years and had no problem with the seal staying in place. It is a very positive connection as you would expect from any rubber gasket between metal and glass. Perhaps those who have had problems are not holding it together firmly while shaking as professional bartenders do. This is simply an improved version of the loose pub style glass used with a metal mixing cup, which is the classic method of cocktail shaking. Just ask James Bond. The insulating properties of the double wall allow the shaker to be used several times over say, 30 minutes, with very little drop in temperature and without having to replenish the ice. The markings on the glass are as clear and crisp as they were the day I got it. The best thing is the quality of the sound it makes when shaking. which for me, is an important component of the ritual of preparing a cocktail.Date published: 2013-07-19Rated 5 out of 5 by tmullett from A step above the traditional barware! I ordered this for my future son-in-law and received it on time with perfect monogram addition. This feels solid and presents well on the bar. The reduced condensation feature is a nice feature. The glass graduate is easy to read and feels solid when locked into the stainless shaker. It nests together to reduce the footprint/storage needs. A great tool!Date published: 2013-05-22Rated 5 out of 5 by SeaglassSusan from So good a bought a second one I used one of these, that fortunately for me was still in the box, at a snowbird rental. It was just what I had been looking for. The seal works well, I wash it by hand to keep it that way, the measurements are just that, ounces and milliliters, not someone else's silly cocktail recipe. I bought one for myself as soon as I got home and now that we have our own snowbird house, I just bought one for there too.Date published: 2013-01-17


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Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation
- First Online:
- Received:
- Accepted:
DOI: 10.1007/s00253-014-5715-6
- Cite this article as:
- Hwang, IW., Makishima, Y., Kato, T. et al. Appl Microbiol Biotechnol (2014) 98: 8201. doi:10.1007/s00253-014-5715-6
- Citations
- 836 Downloads
Biotin-dependent human acetyl-CoA carboxylases (ACCs) are integral in homeostatic lipid metabolism. By securing posttranslational biotinylation, ACCs perform coordinated catalytic functions allosterically regulated by phosphorylation/dephosphorylation and citrate. The production of authentic recombinant ACCs is heeded to provide a reliable tool for molecular studies and drug discovery. Here, we examined whether the human ACC2 (hACC2), an isoform of ACC produced using the silkworm BmNPV bacmid system, is equipped with proper posttranslational modifications to carry out catalytic functions as the silkworm harbors an inherent posttranslational modification machinery. Purified hACC2 possessed genuine biotinylation capacity probed by biotin-specific streptavidin and biotin antibodies. In addition, phosphorylated hACC2 displayed limited catalytic activity whereas dephosphorylated hACC2 revealed an enhanced enzymatic activity. Moreover, hACC2 polymerization, analyzed by native page gel analysis and atomic force microscopy imaging, was allosterically regulated by citrate and the phosphorylation/dephosphorylation modulated citrate-induced hACC2 polymerization process. Thus, the silkworm BmNPV bacmid system provides a reliable eukaryotic protein production platform for structural and functional analysis and therapeutic drug discovery applications implementing suitable posttranslational biotinylation and phosphorylation.
KeywordsHuman acetyl-CoA carboxylase 2 (hACC2) Phosphorylation/dephosphorylation Lipid metabolism Silkworm Bombyx mori nucleopolyhedrovirus
Acetyl-CoA carboxylases (ACCs) are biotin-dependent enzymes catalyzing the production of malonyl-CoA from acetyl-CoA, a critical metabolic intermediate in lipid metabolism (Brownsey et al. 2006; Kim 1997; Saggerson 2008; Tong 2013; Wakil and Abu-Elheiga 2009). Two different isoforms of ACC, ACC1 and ACC2, partake in lipid metabolism in humans and mammals (Abu-Elheiga et al. 1995, 1997; Ha et al. 1996). ACC1, encoded by ACACA, predominantly exists in the cytosol of lipogenic organs such as adipose tissue and liver where malonyl-CoA functions as a substrate for long chain fatty acids synthesis. In contrast, ACACB-encoded ACC2 is associated with the outer membrane of mitochondria in oxidative tissues such as the heart, liver, and skeletal muscle where malonyl-CoA is utilized as a negative regulator of fatty acid oxidation. Due to the bifunctional roles in catabolic and anabolic metabolism, ACC functions as a bioenergetics controller to promote stem cell function and tissue regeneration to regulate lipid homeostasis (Folmes et al. 2013; Fullerton et al. 2013; Knobloch et al. 2013; Park et al. 2013). Moreover, ACC2 knockout demonstrates anti-obesity effects and prevention of cardiac remodeling (Abu-Elheiga et al. 2001, 2003; Kolwicz et al. 2012). Therefore, ACC activity regulation has been recognized as an attractive therapeutic target for dysregulated lipid metabolism such as obesity, diabetes, cancer, and cardiovascular disease (Tong and Harwood 2006).
The baculovirus expression system has been considered as the most efficient eukaryotic heterologous protein expression system as the host insect cells can implement foolproof posttranslational modifications similar to higher eukaryotes (Kost et al. 2005; Possee 1997). Two types of baculovirus expression systems, i.e., Autographa california multiple nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) systems, have been widely used (Kost et al. 2005; Maeda 1989). We developed a BmNPV bacmid, an Escherichia coli and B. mori hybrid shuttle vector, to expedite the heterologous protein production platform without construction and amplification of viruses in B. mori culture cells as recombinant BmNPV DNA can be directly injected into silkworm pupae or larvae (Hiyoshi et al. 2007; Motohashi et al. 2005; Park et al. 2008a). Using the BmNPV bacmid system, intracellular, extracellular, and membrane proteins have been successfully generated with proper folding and posttranslational modifications (Kato et al. 2010, 2012; Otsuki et al. 2013).
Here, we examined whether the recombinant human ACC2, produced using the silkworm BmNPV bacmid-based approach, secures proper posttranslational modifications to fulfill the essential catalysis and allosteric modulation. We report that ACC2, demonstrating consistent catalytic activities with proper posttranslational biotinylation and phosphorylation, is regulated by allosteric modulation. Thus, silkworm-based BmNPV system provides a reliable large-scale protein production platform for structural and function studies as well as drug discovery applications implementing essential posttranslational modifications.
Materials and methods
Construction of recombinant hACC2 BmNPV bacmid
The human ACC2 (2,548 amino acids) is associated with the mitochondria membrane through N-terminal 148 hydrophobic amino acids classified as mitochondrial attachment and target sequences (Tong 2013). To increase the solubility of recombinant human ACC2 (hACC2), these hydrophobic residues were excluded using polymerase chain reaction (PCR) with the following set of primers, 5′-GCCGTCGACATGTCCAAAGAAGACAAGAAGCAG-3′ (forward), 5′-GCTCTAGATTACTTGTCATCGTCATCCTTGTAGTCGGTGGAGGCCGGGCTGTCCATG-3′ (reverse), and designated as Δ148aa-hACC2. The PCR cycle was performed following 30 cycles of denaturation at 94 °C for 30s, annealing at 55 °C for 30s, and extension at 72 °C for 7 min, followed by a final extension at 72 °C for 10 min. The complementary DNA of human ACC2 from Mammalian Gene Collection (Thermo Scientific, Pittsburgh, PA, USA) was used as a template. The resultant PCR product was digested with SalI and XbaI followed by purification with a GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare, Amersham, UK). The purified DNA fragment was ligated into pFastbac 1 vector, which was transformed into E. coli competent DH5α cells (Invitrogen, Carlsbad, CA, USA) and cultured on a solid LB medium containing 100 μg/mL of ampicillin at 37 °C for 18 h to generate recombinant plasmid. The plasmid containing human Δ148aa-hACC2 gene was isolated and identified by DNA sequencing. Finally, E. coli BmDH10bac-CP–-Chi– competent cells containing the cysteine proteinase- and chitinase-deficient BmNPV bacmid (Park et al. 2008a) were transformed with the pFastbac1-Δ148aa-hACC2 and cultured on a solid LB medium containing 50 μg/mL of kanamycin, 7 μg/mL of gentamycin, 10 μg/mL of tetracycline, 40 μg/mL of isopropyl β-d-1-thiogalactopyranoside (IPTG), and 100 μg/mL of 5-bromo-4-chloro-3-indolyl-4-galactoside (X-Gal) (Takara Bio Inc., Otsu Shiga, Japan) at 37 °C for 18 h. The bacmid containing BmNPV-Δ148aa-hACC2 was isolated from white positive colonies.
Expression and purification of recombinant hACC2 in silkworm
Silkworm pupae were used for the expression of recombinant Δ148aa-hACC2 as a bioreactor. To produce recombinant protein in pupae, 10 μg of BmNPV-Δ148aa-hACC2 bacmid DNA was directly injected with DMRIE-C reagent (Invitrogen) into the dorsal of pupae. The injected pupae were reared at 27 °C for 6–7 days, and stored at –80 °C until further analysis. Protein purification was carried out at 4 °C to minimize aggregation and protease activity. Five pupae were homogenized in 10 mL of lysis buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.4, and 0.1 % TritonX-100) containing an EDTA-free protease inhibitor tablet (Roche, Mannheim, Germany) using a homogenizer (GLH-115, Yamato, Tokyo, Japan). Cell debris was removed by pelleting through centrifugation at 12,000 × g for 30 min. The supernatant was filtered using a 0.45-μm syringe filter and loaded onto a 500-μL of Anti-FLAG M2 antibody Affinity Gel (Sigma-Aldrich, St. Louis, MO, USA) pre-equilibrated with equilibration buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.4 and 0.02 % TritonX-100). The column was washed with 2.5 mL of equilibration buffer and eluted with elution buffer (100 μg/mL FLAG peptide in 50 mM Tris-HCl and 150 mM NaCl, pH 7.4). The eluted Δ148aa-hACC2 was collected and concentrated using a 100 K Amicon Ultra centrifugal filter (Millipore, Billerica, MA, USA).
Confirmation of biotinylation and phosphorylation by Western blotting
The posttranslational biotinylation and phosphorylation of purified Δ148aa-hACC2 were measured by Western blotting analysis. Prior to electrophoresis, purified sample was boiled for 5 min at 95 °C with protein denaturing buffer (Nacalai Tesque, Kyoto, Japan). Samples were electrophoresed in a 5 % SDS-PAGE gel with the Mini-protean system (Bio-Rad, Hercules, CA, USA) at 150 V for 45–60 min in Tris-glycine buffer (25 mM Tris, 250 mM glycine, pH 8.3, and 0.1 % SDS). The separated proteins on a SDS-PAGE gel were transferred to PVDF membranes (GE Healthcare) by electroblotting on a wet blotter (Bio-Rad) at 15 V for 1 h. To detect the purified Δ148aa-hACC2 and their biotinylation and phosphorylation, several specific antibodies were used. A mouse anti-FLAG antibody (Wako Pure Chem. Ind. Ltd., Osaka, Japan) was used to detect purified Δ148aa-hACC2 as a primary antibody. A monoclonal anti-phosphoserine antibody (Sigma-Aldrich) was used for phosphorylation detection as a primary antibody. An anti-mouse IgG-HRP (GE Healthcare) was used for above both cases as a secondary antibody. A goat anti-biotin antibody (Abcam, Cambridge, MA, USA) and streptavidin HRP conjugate (Thermo Scientific, Rockford, IL, USA) were used for biotinylation detection as a primary antibody. A rabbit anti-goat IgG-HRP (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and an anti-mouse IgG-HRP were used as a secondary antibody.
Dephosphorylation of Δ148aa-hACC2
Dephosphorylation was carried out using Lambda protein phosphatase (Lambda PP; New England Biolabs, Ipswich, MA, USA). The purified Δ148aa-hACC2 was incubated with 0.5 μL of Lambda PP, 1X NEBuffer for protein metallophosphatases (PMP) and 1 mM MnCl2 at 30 °C for 0, 1, 3, 6 h. Sterilized water instead of Lambda PP was used as negative control. Dephosphorylation was confirmed by Western blot using a monoclonal anti-phosphoserine antibody (Sigma-Aldrich) produced in mouse and an anti-mouse IgG-HRP (GE Healthcare). The activity of dephosphorylated Δ148aa-hACC2 was determined using ACC assay.
Acetyl-CoA carboxylase assay
To measure ACC activity, 4 μL of purified Δ148aa-hACC2 was incubated with 36 μL of reaction buffer (50 mM of HEPES, pH 7.4, 5 mM of NaHCO3, 10 mM of MgCl2, 10 mM of sodium citrate, 0.5 % of DMSO, 4 mM of ATP, and 0.4 mM of acetyl-CoA) at 37 °C. The reaction was terminated by addition of 4 μL of 100 % trichloroacetic acid. The produced phosphate during the reaction was determined using a SensoLyte® MG Phosphate Assay Kit (AnaSpec, Fremont, CA, USA) by measuring the absorbance at 655 nm. Protein concentration was determined using BCA protein assay kit (Thermo Scientific).
Citrate-induced polymerization of Δ148aa-hACC2
In order to confirm the allosteric regulation of purified Δ148aa-hACC2 from silkworm, citrate-induced polymerization was evaluated. The purified Δ148aa-hACC2 was incubated with 50 mM of HEPES (pH 7.4), 1 mM of dithiothreitol (DTT), and different concentration of citrate at 37 °C for 30 min. The polymerization was evaluated by Native-PAGE and Western blotting. Purified proteins were prepared in a non-denaturing sample buffer (Native sample buffer, Bio-Rad). Samples were electrophoresed in a 5 % Native-PAGE (without SDS) with the Mini-protean system (Bio-Rad) at 150 V for 45–60 min in Tris-glycine buffer (25 mM Tris and 250 mM glycine, pH 8.3). Next, Western blotting protocol was used as described above. The antibodies for detecting polymerized Δ148aa-hACC2 were same as used for detecting purified Δ148aa-hACC2. The activity of Δ148aa-hACC2 by citrate concentration-dependent polymerization was determined by ACC assay.
Atomic force microscopy
Nanoscale AFM imaging was employed to investigate the dynamic forms polymers due to allosteric regulation of hACC2 by citrate. The Δ148aa-hACC2 was incubated with or without 15 mM citrate for 20 min at 37 °C. The resultant mixtures were placed on the freshly cleaved mica surface and incubated for several hours in a moisture chamber. After washing with water and drying under nitrogen, the samples were subjected to tapping mode AFM imaging on the Nanoscope IV PicoForce Multimode AFM, equipped with an E-scanner and a rectangular-shaped silicon cantilever (Bruker, Madison, WI, USA) with a 42-N/m spring constant and a resonant frequency of ~300 kHz at ambient environment (Park et al. 2008b; Park and Terzic 2010). Images (512 × 512 pixels/image) were collected from each sample with maximum image size of 5 × 5 μm and analyzed using the Nanoscope Version 6.13 software (Bruker).
Expression and purification of recombinant Δ148aa-hACC2
Biotinylation of Δ148aa-hACC2
Phosphorylation and dephosphorylation of Δ148aa-hACC2
Adenosine monophosphate-activated protein kinase (AMPK)-mediated phosphorylation is other layer of posttranslational modification to allosterically regulate ACC catalytic function. Phosphorylation inactivates ACC catalytic activity whereas dephosphorylation activates the enzymatic function. Notably, phosphorylation of Ser222 in hACC2 (Ser212 in mouse ACC2) has been recognized as a vital process for homeostatic lipid metabolism (Fullerton et al. 2013; Wakil and Abu-Elheiga 2009). Consistent with these findings, the crystal structure of biotin carboxylase domain of hACC2 has revealed that the phosphorylation of Ser222 disrupts the polymerization of ACC2, a widely recognized mechanism in modulating catalytic function (Cho et al. 2010; Lee et al. 2008).
The effect of dephosphorylation was assessed by measuring the catalytic function of Δ148aa-hACC2 (Fig. 4b). Δ148aa-hACC2 was treated with and without Lambda protein phosphatase for 2 h, and then catalytic activity was measured as a function of incubation time. Purified Δ148aa-hACC2 with indigenous posttranslational phosphorylation provided a specific activity of 0.786 ± 0.229 nmol Pi/mg/min (n = 6), whereas phosphatase treated Δ148aa-hACC2 protein yielded a specific activity of 1.336 ± 0.441 nmol Pi/mg/min (n = 6), about 2-fold increase. These measurements are consistent with the findings observed in knock-in mice samples where Ser212 (mouse sequence) is replaced with alanine to ablate the critical serine phosphorylation (Fullerton et al. 2013).
Allosteric activation of Δ148aa-hACC2 by citrate
Citrate-induced polymerization has been extensively employed to understand the regulatory mechanism of ACC, although the concentrations of citrate required for allosteric activation are much higher than that present at physiological locale (Beaty and Lane 1983a, b; Gregolin et al. 1966; Kim et al. 2010). Upon incubation with citrate, ACC polymerizes into filamentous structures containing 10–20 protomer units with increased functional activity (Kim et al. 2007; Locke et al. 2008). Regardless of biological significance of citrate in ACC regulation, the citrate binding sites have not been identified.
Acetyl-CoA carboxylase is a multidomain and multifunctional protein working as an energetic controller in homeostatic lipid metabolism participating in fatty acid synthesis and fatty acid oxidation (Tong 2013; Wakil and Abu-Elheiga 2009). The catalytic function of ACC through a biotin prosthetic group can be allosterically regulated by multiple factors including posttranslational phosphorylation and dephosphorylation (Wakil and Abu-Elheiga 2009). In addition, tertiary level regulation of ACC with small acidic proteins, i.e., Spot14 and Mig12, has been recently identified (Colbert et al. 2010; Kim et al. 2010; Knobloch et al. 2013; Park et al. 2013). Particularly, due to the pathophysiological relevance of ACC2 in lipid metabolic syndrome associated with obesity, diabetes, cancer, and cardiovascular disease, ACC2 activity regulation has been considered as a candidate target for therapeutic interventions, which essentially requires authentic bioengineered recombinant proteins (Abu-Elheiga et al. 2001; Tong 2013; Tong and Harwood 2006). Here, we successfully produced with high fidelity human ACC2 using the silkworm BmNPV system armed with a proper posttranslational modification machinery. The heterologous ACC2 harbors all necessary posttranslational biotinylation and phosphorylation, vital to maintain functional integrity. Thus, the silkworm BmNPV bacmid system provides a reliable large-scale production platform for eukaryotic proteins required for posttranslational modifications.
Biotin is a water-soluble vitamin serving as a vital prosthetic group involved in five carboxylases in human (Zempleni et al. 2009). In hACC2, biotin is covalently linked to lysine 929 within a BCCP domain and forms carboxybiotin using bicarbonate as the CO2 donor. Following a large conformational change, the carboxyl group from carboxybiotin is transferred to acetyl-CoA to produce malonyl-CoA (Tong 2013). We revealed posttranslational biotinylation of functional recombinant hACC2 from silkworm, without any additional supplement of biotin under silkworm rearing conditions. We believe that this is the first demonstration of posttranslational biotinylation in proteins expressed in silkworm B. mori.
ACC phosphorylation/dephosphorylation is one of the allosteric regulatory mechanisms. Phosphorylation of ACC by AMP-activated protein kinase and cAMP-dependent protein kinase inhibits the enzymatic activity of ACC, whereas dephosphorylation activates the catalytic function (Munday et al. 1988). Although several phosphorylation sites have been identified, Ser212 (mouse sequence) phosphorylation was recently recognized as an integral process in activity modulation (Fullerton et al. 2013) where knock-in mice with substitution of Ser212 with alanine displayed an increased ACC2 activity. Consistent with this, we demonstrated about a 2-fold increased specific activity in dephosphorylated hACC2 compared to the phosphorylated counterpart, underscoring that the critical serine residue is fully accessible to phosphatase protein. ACC is also allosterically activated by citrate, which is a metabolic intermediate produced in mitochondrial tricarboxylic acid cycle. Although the concentrations of citrate required to increase the enzymatic activity of ACC are much higher than the physiological concentrations of citrate, citrate has been widely used to modulate ACC function (Beaty and Lane 1983a; Cheng et al. 2007; Thampy and Wakil 1988). We demonstrated herein citrate-induced hACC2 catalytic function enhancement and filamentous polymer formation. In summary, a multifunctional human ACC2 was successfully produced using silkworm-based protein expression system. Heterologous ACC2 was correctly folded with posttranslational biotinylation and phosphorylation, retaining catalytic activity and citrate-induced allosteric regulation. Moreover, silkworm demonstrated a high yield of recombinant ACC2 production. Thus, the silkworm-based BmNPV expression method equipped with a proper posttranslational modification machinery provides a large-scale eukaryotic protein production platform for structural and functional research particularly in the application of therapeutic drug discovery.
This work was supported partly by the Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation from Japan Society for the Promotion of Science (JSPS), Japan, the National Institutes of Health, and Marriott Heart Disease Research Program, Mayo Clinic. A.T. holds the Marriott Family Professorship in Cardiovascular Diseases Research.
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Nashua Telegraph (Newspaper) - September 24, 1969, Nashua, New Hampshire Today's Chuckle The best time for parents js when the children are too old-to cry and too young to borrow the car. Largest Evening Newspaper... d' 'M Weather Clear, Cool. Tonight Breezy, Cool Thursday, VOL 101 NO. 174 Continuing New Hampshire Telegraph Established October r NEW HAMPSHIRE, WEDNESDAY, SEPTEMBER 24, mi UPASES Pne. TEN CENTS Mayor Stalls Court Action On Petition B) CIAUDETTE DUROCHER Mayor Dennis J. Sullivan has decided to delay until Sept. 30 any court action which would compel the aldermen to put the Neverett property purchase on the Nov. 4 ballot. In an unexpected development, the mayor told the Board of Aldermen last night he would "instruct my attorney to proceed and issue these (mandamus) writs on the morrow, unless you, the board, choose to respect and honor the legal local citizenry's right to vote" on the property purchase. Retain Stand dum questions on the ballot is up But Sullivan's latest volley over the issue failed to pierce the aldermen's resoluteness in shelv- ing the stalled Neverett prop- erty initiative petition. His delay in seeking court ac- tion, SuUiyan said, resulted in a conference today with his attor- ney, Aaron A. Harkaway. The 'mayor said it was agreed to hold on the filing of a man- damus petition in Superior Court in the hope that the aldermen will reconsider their shelving of his initiative petition by Sept 30. Reading from the city charter last night, Sullivan noted that the aldermen have 20 days to act on an initiative petition, bringing their deadline to that date. j He said today he stands ready to call a special meeting if the alder- men are prepared to reconsider.! A petition of mandamus asks I the court to direct an officer to discharge his duties. In the pres- ent the petition would be to the aldermen's discretion. In requesting the aldermen to reconsider their shelving of the petition, Sullivan called Gormless reasoning' as "most question- able "I have contacted the secretary of state and olher legal counsel arid have been advised that this question, 'whether it be described as a measure or.not' can be placed on the ballot this Novem- ber up to 12 hours previous to the day of election." MAYOR STALLS Page! This was a general view in North Chelmsford, Mass, yesterday after 13 freight cars in the midd_le of a Boston arid Maine freight' train. derailed at 'a grade crossing. The mishap was the, 39th derailment :in 'the last nine months on B M tracks in the state. William L. Webster, 53, of tewksbury was killed when a heavy-duty, crane, he was operating 'tipped over .while clearing debris. (AP Wirephoto) 24 jo 79 Victory GOP Senators Elect Scott By WALTER R. HEARS .Scott has held that No. 2 post WASHINGTON filed in Superior Court and the Hugh Scott of Pennsylvania' was tiSFa aldenhen would then be served elected today leader of the Sen leaderTSached off a'fol- with notices filing. of the petition's ate Republican minority. Both sides lo the dispute would be heard by the court before it on'whether the petition for a writ mandamus should be grant- ed. The court usually gives prece- dence to requests' of this type but it is not known how much time would elapse before a decision would be rendered. In taking the initiative petition issue to'court, Sullivan would acting.as a private citizen and would have to bear legal costs in- volved. He aldermen's only response last night to the mayor's chal- lenge was to ask City Solicitor Arthur 0. Gormley Jr., if they should take any action in the wake of Sullivan's renewed efforts to have the property taking put on the ballot Denies Obligation Gonnley, in answering ques- tions posed by Alderman-at- Large Donald R. Hardy, stressed the aldermen are under no legal obligation to put the so-called initiative petition on the ballot. As he said two weeks earlier in rendering_ a lengthy opinion on the petition, Gormley stated that according to his interpreta- tion of the city charter require- ments, the petiticn is not a true initiative petition but should be considered instead as a referen- dum petition. And, he added, putting referen- Scott defeated Sen. Howard H. Baker Jr., of Tennessee, Zl't'o 19, at a closed door conference of Senate Republicans. The electtba'-efthe 6S-yeaf-cld Permsylvanian to succeed the late Sen. Everett M. Dirkse'n opened a new leadership vacan- cy-that of GOP whip.'. low-up campajjnf for.whiPf Sjstt, the idr'BgSting forpramMMra'f; the No. J GOP the the vote tttflif'roppwl was and tufll- cient lot .victory.; r: 1 .first-term senator from Insisted he was Mead bat jald neither can- I Mrs. Hicks Leads Field in Comeback BOSTON (AP) Mrs. Louise Day Hicks, whose political No's have made news for years, made a rousing start toward a come- back by outrunning the field for city council in Tuesday's prelimi- nary election. The 50-year-old grandmother who as school committee chair- man fought busing to end racial segregation in public schools and still is fighting the state's school racial balance law, out- polled her nearest rival by votes in an unofficial count. She has been out of office since she ran for mayor two years ago and lost to Kevin H. White. Always' a strong vote-getter in her native city; Mrs; Hicks dem- onstrated her strength 'anew and threatened a' formidable barrier in the way of one'of the nire incumbent councilors seek- ing reelection Nov. 4. All of the nine incumbents were nominated among the 18 whose names will be on the bal- lot in November, but a few ran well down the list. Councilor Thomas I. Atkins, for instance, the council's only Negro member, ran 12th in a ifield of 23. Council President Gerald F. O'Leary was Sth and Councilor Garrett M. Byrne, son of Suffolk County Dist. Atty. Garrett H. Byrne, was 15th. didate had the advance commit- ments to insure victory. Bpthjiad discounted lingering tali that the 12-day campaign for the :post might wind up In a position a fresh- man with only years tn a Congress built around the. sen- iority system! Republicans generally have stilemafe that coiild throw: jjiorf cpnserva'trf'e seMK itectibnio a tors in reont years to lead theft Senate sayi as a hall dozen sena- tors: refused to commit. thein- seltes openly" to.-' either, of'the candidates -In advance of to- secret baDot- post left vacant by of'Sea Everett M. Dirkseri. The Illinois senator who had been Republican leader for a decade, died Sept 7. Although none of the hopefuls wpnld say anything publicly im- tri after Dirksea's burial, there was considerable early back- stage maneuvering by their supporters. .'Baker, Dirksen's-son-in-law, announced his des'ire for the jost Sept.-12 immediately upon Washington after the funeral. Scott quickly confirmed he was in the running. A third entry, Sen. Roman L. Iruska of Nebraska, dropped out after a week of campaigning when he failed to turn up suffi-, cient support. He endorsed Bak-i Js conservative 6n': tome 'is- sues aridj :pi: 'others, that 'tradition. But some mort lenior seal- tors' balked at the idM of hind- In Food Program Ing top fob to' a nan elected to .the Senile '.only once.: CONCORD, N. H. (AP) Top county officials, re- "rairiing' from: r discussing a hunger report at: the re- quest of Gov. .Walter Pet- erson, cited "red in- !ekible regulations, the uctance of applicants and problems ,in storage and ransportation as stumbling docks; to surplus food dis- ribution. Wants Facts Peterson opened the meeting f about 50 county commission- rs, legislators and executive ouncilors with the 'admonition ie did not want to .discuss the icrits of a recent Office of Ec- onomic Opportunity rreport but anted and first and knowledge." Problems cited in reports by rtpresentativei ol each comity eluded: .a by icme eedy persons to .apply for food under the U.S. Department of griculture's surplus food pro- ram. .the failure of some towns become involved distribution. too'inuch paperwork. .lack .'of: adequate, staff embers a'dminis- r th'e'prpgram. v> v in'iome o dequate storage 'space an .raasportitlon .'for.the food: .to i'e counties and stringent regulations whlc allow, httie discretion on the whose recipients automatically would qualify. in some instances, improp- er use of the food after it reaches the needy persons. The meeting stemmed from reaction to an OEO report com- piled by University of New Hampshire researchers. The re- port, entitled "The Curse That charged abuse of the program and indifference lo the problem by some officials. "When there are complaints from organizations, I wish they would bring them to us and let us digest them and see what we can said Russell Hall of Rockingham County. He said some towns "just don't want to be involved" but lhat the county provides for dis- tribution .of surplus food in those areas where the town re? fuses to participate. He said many town .officials, consider it "too much too much Peterson .asked Hall, thought the person's dispensing the foods are "understanding and "I t ry to make them feelat Hall said, noting he had moved his office so, the needy could collect the food in priva- cy. ''I try to make them feel they are people, not hs said. "It's humiliating enough to come into my office." Armand Beaulieu -of Hills- borough County, answering a ques- tion by Councilor Stephen Smith, said in some instances.the food is properly used ,bat in' other! FOOD PROGRAM PageJ d iick af-propir.'communld agencies Official :er No unosu'al amount of banger is present in County Armand A. Beaidieu, county com- missioner, said today., Referring to the meeting in Concord which Gov. Walter Peterson and-his Executive Council conferred wift county commissioners from all over the state, Beaulieu said, he (believed the recent report on "The campaign for the post- hunger in New Hampstdre was waged in conversation and conference, in the cloakrooms 3 and to in- olved two key. ingredients: rating as among lib- eral Republicans. He said, "My recommendations to the 'governor are that possibly the towns served by Nashua and Manchester could be better served if depots and distribution Task Force Group Urges Educational Changes CONCORD, N.H. (AP) -Sweeping changes in New Hampshire's educational system have been proposed by a development anc mental health. Recommendations also include letting out contracts on paper work such as vocational-technical education opportunities and strengthening the Department of Education. The subcommittee report unions. AIM i can see that soon we may have as few as 38 or 26. But I think it will take a long time for us to see fewer than that." commit tee 'of the citizens inventory to private to the committee on commissioner called the slate standards development and then on recommendations The report issued salaries and executive committee for noting that "many of recommends reducing the some textile and other suggestions in it were ber of school for school use. Reaction lo the report from 41 to five. Each of report also says Commissioner said another subcommit- five districts would have age 14 should was generally was suggesting the setting elected school board and forced to continue their the bulk of the of 17 regional areas and that correspond with olher courses if they want Paire did say reduction of supervisory un- and planning agencies such in work-study some reservations to five "is not consistent '69 Chevrolets CARS TRUCKS Daily Long-range recommendations include a state system of day care centers and plan to reduce the number of superintendencies. "I have some serious reservations lhat report." He added: "I think the report indicated our department had some liml- as low one suggesuon. i imnjc n will be a long time in 1 SAVE MORE he IN per day Call Teri OIL CO. INC. SERVING -NASHUA one time we had 65 TELEGRAPH ONLT MacMulkin TOWNS DEALER 24 1 Horoscope H Anderson 4 Nashua Scene 4 Baker .23 Obituaries 2 PIZZA by Charles Famous thruout New England Iff W. PEARL ST. Fines! in Pizzas FREE SHOP SATURDAYS AH Day In Downtown Nashua close (o 300 businesses 1 V 1 W Sti-Doo Suits' Boots Trailers Sleds Accessories Parti Nashua Auto Co. Outdoor Recreation 23 Sports 36, 37 Classifieds Suburban 10, 11 39. 40. 41, 42, 43 Taylor Comics 39 Television 18 Cromley 4 Theaters 38 Crossword 27 Dr. Thoslcson 34 Editorial 4 Weather 2 (all Main Street, Nashua, N. 6 Regular 90c PI Experts TRUST has TUESDAY plus Green paying daily interest on 5% ONLY J Telephone Nashua Wallpaper Deposit accounts since 1967. i Dp.n 1 1 A.M. to 2 W. Pearl St. UEMBEB Men. thru thru Sat. Sundayt 3 P.M. fo Ttars. 'tfl _______________ tations by virtue of the fact we lack some key people. I noted that the report sev- eral people be added to assist in serving the he said. "These were our recommen- dations in our supplementary he added, "I havetroleum Kennedy old the Senate committee. Claims Request Dent, chief White House politi- cal liaison, could not be reached or comment. But his letter, written In response to a resolu- on from the governing board of the oil-rich Texas county urg- ing Nixon to' stand fast .for -2754 per cent, said .specifically. President "has asked me spond to his behalf." t- It was 'addressed lo Mrs. head bM thd commissionefjt in Midland County, .a West Texas area where _oil Is' the major cash crop. Mrs. .Culver is' an active two- term. Republican in an area with strong conservative senti- ment. The .'initial acceptance of allowance 'Cut bore the unmis- iakable imprimatur of jdent. It was .hashed but' in many conferences involving much of Nixon's staff and cul- minated with most of the Treas- ury's leaders going to the West- ern White House in mente, Calif., for San Cle- personal conferences wth the President and his' staff. To heighten the mystery :aused by the Dent letter, a canvass of Treasury Depart- ment sources turned up no ons who would admit knowing of the ewest policy change indicated ly what, the presidential assist- ,nt wrote. However, the Treasury ources did not seem too sur- ristd such a policy changa "ould be made without theif jiowledge. The 27H per cent re- ult of an ancient compromise wtween the Senate and House been under attack with ritics saying it is a symbol o! ax privileges granted big bud- ess by the government. Architects For School Interviewed The architect selection commit' lee for the new "super" high school Bill re-interview several architectural firms Oct. 15. Committee members recently questioned representatives of 2} architectural firms in a three- day series of Interviews. A committee spokesman said the aim of the new interviews Is to narrow down the field for selec- tion of an architect design the school. Herbert E. Miller, school board member, is committee chairman. Members include board members Dr. J. Gerard Levesque, John.T. Dimtsios, AWermen-at- Urge Maurice L. John V. Wesson and Alderman Donald L Ethier. The group is to report its rec- ommendation io the Joint school bunding which wfll actually architect. SST Gefs Go-Ahead Transportation Secretary John Volpe discusses the. go-ahead by, the White House for construction of a supersonic transport plane in the next five years. With'the Secretary at the White House, are from left: Henry Jackson, D-Wash., Washington Gov. Daniel Evans, FAA Administrator John Shaffer, and Transportation James Biggs.' (AP Wirephoto) ;
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text:
I'm Basically A Native
By Jenna Booth
I'm not walking around saying "brekkie" instead of "breakfast", or "too easy" instead of "no problem", but I already have a grip on living in Sydney after about one week.
I know how to get places!
Whether it's walking around at night seeing the light and music shows being put on by Vivid or running through the city and its many parks this morning, I already have a sense of the city and where I am.
I have utilized every sort of transportation:
The arduous but surprisingly manageable 22 hours spent on an airplane
The ferry to Manly beach (once for a Food, Wine, and Sustainability Festival; once to go hiking on the cliffs)
Multiple train rides
Bus rides
A taxi to Salesforce.com when it was pouring rain
Walking everywhere
I know where to get food!
Australia has two large grocery store chains: Woolworths and Coles, the latter of which is on the block catty-corner to the block our apartment is on and I can find a few gluten-free/dairy-free products to satisfy my unfortunate diet. The GF/DF cookies are better in the U.S., but the GF/DF bread in Australia beats the American counterpart any day.
Since the major chains can by kind of pricey, I buy all of my produce at Paddy's Market on Sunday afternoons. It's basically an extremely crowded farmer's market in the basement of Market City in Chinatown. Our apartment is located just outside of Chinatown and there are at least ten Thai restaurants within two blocks.
I am no longer fooled by forecasts!
It says it is going to rain just about every day and it rarely does. Somehow all the Australians know that it isn't going to rain. What is this sorcery?
But I still do tourist-y things.
Like taking pictures of everything.
Like having to ask a million questions about what buses go to which places.
Like almost buying a cheap sweatshirt with "AUSTRALIA" plastered on it.
Like going to bed at 9pm sometimes because I'm still jet-lagged.
Like stressing over my extremely limited access to Wi-Fi.
Like climbing the Sydney Harbor Bridge.
*The influence and prevalence of Asian culture is very strong in Sydney; meanwhile, I can count on one hand the number of black people I have seen here.


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Simple and natural coastal style craft, home decor, DIY and yarn arts ideas.
Unleash your natural creativity with simple and inspiring craft, home decor, DIY and yarn arts ideas. With detailed step-by-step DIY tutorials and videos we'll have anyone saying "I can do it" in no-time. With sustainability in mind we use natural materials such as driftwood, wool, beach glass and more to create coastal and nature-inspired projects. We also transform, repurpose and upcycle thrift store, curbside and yard sale finds for the love of our homes, kids and selves. From home decor and organization ideas, crochet and knitting patterns, recipes and more, we help our readers express their creative side.
Sustain My Craft Habit | , Toronto, ON


text: In a classic "Peanuts" comic strip, Charlie Brown goes to Lucy for psychiatric help. He says, "What can you do when you don't fit in? What can you do when life seems to be passing you by?" Lucy leads Charlie away from her booth and says, "Follow me. I want to show you something. See the horizon over there? See how big this world is? See how much room there is for everybody? Have you ever seen any other worlds?" Charlie replies meekly, "No." She continues, "As far as you know, this is the only world there is...Right?" Even more meekly, Charlie says, "Right." Lucy presses on, "There are no other worlds for you to live in...Right?" Charlie admits, "Right." "You were born to live in this world...Right?" "Right," says Charlie. Lucy then explodes, "Well, live in it then! Five cents, please." While we may disagree with Lucy's counseling technique, we recognize she is on to something. We need to make the most of our lives and really live. Sometimes this is easier said than done and sometimes we need help with it. In either case, I'm here for you.


text: They've done a fine job with their rollout issue, though I hope they don't end up sacrificing their credibility on the altar of becoming some kind of cheesy "lifestyle" rag. No offense to those with dissenting views, but I'm not sure that we really need a gun-centric version of Maxim. There are some early indications that they're headed that way regardless, and given the way that advertising revenues work, I guess I shouldn't bother to feign surprise when that happens. Whatever the case, the January 2012 issue is worth your time.


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The profile picture I have up right now is an unfinished drawing which also looks much more yellow than it is, also looks better in person just in general.
Dear fellow Swifties,
I have redo this about me many times and it keeps changes which I suppose is a good thing.
I am not always good at this but I think we should get one thing straight-I love Taylor Alison Swift. I have been fortunate enough to go to 3 of her concerts, Speak Now, Red, and 1989.
On to me, uh well aside from Taylor I listen to Carrie Underwood, Ed Sheeran, Lucy Hale (and watch her), A Rocket to the Moon, and others.
I think I am gonna list some stuff then...
I am fifteen
I am from New York
I love to draw (the profile is an unfinished drawing of Taylor)
I watch A LOT of TV favorites include- Buffy the Vampire Slayer, Doctor Who, Boy (and girl) Meets World, Pretty Little liars, anything on HGTV, Once Upon a Time, Friends, Chasing Life, and many many more (this is my life, it's fun)
I collect mugs
I love to read, which has become more difficult with life but- Percy Jackson, A Girl Named Digit, (hunger games hence the name) anything by John Green...I can't think of the others.
My favorite animal is an Owl
I am crazy when sleep deprived and it's awesome
I am an Aries
And thinking of it not sure why you would what to read all this, thanks if you did. I strongly admire you for that.
-A Swiftie
Dear Fellow Swifties, Whoa, TC has changed a lot since I've been on. Been too busy listening to 1989...and High School... Let's get to what I think. I LOVE 1989. Still haven't gotten to listen to the bonus tracks and memos-but I have the CD now. Order from least to favorite-1 being my favorite. Put some side notes- 13. Welcome to New York (Ironic since I live just...


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for CPAs
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My Profile
Enrique Pena
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Social media marketing is the process of building awareness about you, your products or services through the various social media channels. The ultimate goal of any social media marketing campaign is to drive traffic to a website, increase the visibility of a product, gain more social media followers or find more customers. The most popular social media networks
buy instagram likes instantly
today are: Facebook, Twitter, Google+, Pinterest, YouTube, Tumblr and Linkedin.It's the fastest way to spread the word about a new product, service or news item – A tweet can go rival in minutes and spread a message around the Word faster than TV or other traditional media It's a trend – Millions of people spend a good amount of their time on social media networks so if you 'need them' you know where to find them It's the new marketing – Back in the old days marketers found out that many people spend time in their cars driving so they thought of the billboards on the roads. Today people are spending time on social media so as a marketer you have to start thinking this way too. It's the new influencer – more and more studies indicate that social media is turning into business as well with more companies stating that they have found customers from Facebook or Twitter.
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text:
Both Sides: Are We All Pre-wired Left or Right? Do Beliefs Precede Facts?
HuffPost Radio | Posted 04.15.2012 | Politics
Read More: Hilary Rosen, Jonah Lehrer, Ann Romney, President Obama, War on Women, Trayvon Martin, Charles Blow, Mitt Romney, Pay Equity, George Zimmerman, Planned Parenthood, Facebook, Contraceptives, Jonathan Haidt, The Righteous Mind, Allen West, Gov. Walker, Arianna Huffington, Isaiah Berlin, Kellyanne Conway, Gaffe-Gate, Bill Maher, Politics News


text:
You missed it This great deal for Le Méridien Dallas, The Stoneleigh is over. But don't despair, we've got more great deals every day.
Built in 1923, this historic hotel in Uptown boasts a luxurious spa that houses a friendly, experienced staff and inviting accommodations that ensure you'll never want to leave:
• $99 ($220 value) for a massage package
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• Call to book your day at the spa
The massage package includes a 50-minute Swedish massage, 30-minute Buff and Glow body scrub, take-home Himalayan bath salts, and valet parking.
The facial package inclues a 50-minute Aroma Essential facial, an Orchid mani/pedi, a spa lunch, and valet parking.
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The Tean restaurant on St Martin's is only accessible by boat, and is open only for eight months during the holiday season.
But fresh lobster and other seafood is landed yards from its door and the views are of clear blue sea.
Head chef Kenny Atkinson, 31, from Newcastle, achieved the award in his first full season since arriving.
He said: "Of course it helps to have the most fantastic ingredients at our back door with a sea larder in front of us.
"When the food that you are cooking with has the same post code as your kitchen you have a distinct advantage."
Mr Atkinson, who has worked at top restaurants in London, said moving to the Scillies with his wife and young son in 2006 had been an easy decision.
"It's beautiful and a great place to have a family and I could still do what I love to do," he said.
The restaurant is one of only 50 outside London awarded stars in England.
Michelin guide editor Derek Bulmer travelled by road, helicopter and boat to carry out his inspection.
He said: "It was probably one of the longest journeys I have ever taken to go and have a meal."
He added: "I think not a lot of people have actually been to the Scilly Isles. It puts it on the foodie map."
St Martin's is one of the five inhabited Isles of Scilly, the largest of which is St Mary's.
What are these?
Most Popular Now | 88,886 people are reading stories on the site right now.";


text: hartford courant Federal Judge in CT Blocks United From Dropping Docs By Kristin Gourlay • Dec 6, 2013 A federal judge in Connecticut is blocking United Healthcare's move to drop hundreds of doctors from its Medicare Advantage network. The Hartford Courant reports that the Fairfield and Hartford County Medical Associations convinced the court that removing the more than 2-thousand doctors in Connecticut from the health insurance network would be too damaging. And that the insurer plans to appeal the decision.


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Separate names with a comma.
Discussion in 'TiVo Mini' started by Bwatford141, Mar 7, 2013.
Who said, from TiVo, that DTA would come to the TP-4 ???
TiVo said:
I know for a fact it is coming to the TP-4s
FWIW, When I said that Tivo "said it was coming" I was talking about discussions and comments made by CSRs (and people here on this site) back when the mini was first released. Not the belated promises - yet to be fulfilled - that they were finally going to do it (via Margarets twitter).
Ok. I will bite. How do you know this for a fact?
I'm sure people have been beta testing it for a while now with the Elite/XL4 boxes.
And RCN's guy confirmed it was a feature of the next release.
I would consider it, although right now I'm in effect charging Comcast's DVR rate to my roommates to use my TiVo stuff, and if I go to DirecTV at some point in the future, then I have to sell my TiVos, so I'd rather not get any more invested in TiVo than I already am...
What is taking Tivo so long to add DTA to the Premier line. It is technically Fall It would be nice if they could speed it up. As is usual, Tivo is sending Roamio updates and putting the earlier series Tivo's on the back-burner.
If it's anything like the Elite's launch, these minor Roamio updates might be Fall beta builds and they're holding back on the bigger fall release until the Roamio-related fires are put out.
If Premiere owners got the update now, it would be a beta build with inherent risks they didn't sign up for. So they're letting the smaller pool of Roamio owners be the testers first. Lol. (Not that I mind, I have some bugs I'd love squashed asap.)
Tivo has been soliciting for beta testers. If you're anxious, sign up and then ping Margret. Maybe she can do you a solid. But it's probably not too far away...
Margret just announced in the Premiere forum that the fall update should be out by the end of the month.
Trying to get a grasp on the anticipated DTA update for Premiere owners:
Currently, I can only watch and/or record on one tunervia the Mini - while the main Premiere still has 3 tuners to use.
So if DTA comes out for the Premiere - does that mean that if the 3 tuners on the Premiere is not being used, that Mini can watch live 1 channel while recording another - i.e. turning it into a 2 tuner Mini?
The mini cannot record anything, it has no hard drive. It can be used to trigger a recording on the Premiere, which is what I think you are actually asking.
Yes. Can the mini be made to watch 1 channel live while 'triggering' record on another tuner?
Currently, I am limited to watch what I am recording
You can press the record button to start recording the channel you're watching, then exit live TV, then go back to live TV and it should grab a different tuner.
It will start the recording on a different tuner than the Mini is using. I know in my use it will typically grab the same tuner I had before when I come back. Unless another recording started between the time I exited out of Live TV and came back. But any recording I start while watching LiveTV from the Mini, has always used a different tuner than the one the Mini was using.
or did the behavior change with the most recent update?
I honestly never tried it. I just assumed that if you hit record on the show you're actively watching via the Mini then it will need to use that tuner because it may need to grab data out of the buffer to get the whole show. If it starts recording on a different tuner then I guess you only get from that point forward instead?
Yes. It would be nice if it used the tuner that you were actively viewing to start the recording. Then it would behave similar to the Roamio. Then if you changed channels it would just grab another tuner.
But right now it does start the recording from the point that you initiate the recording. Although if the tuner it uses is on the same channel, then maybe it puts what's in the buffer in the recording?
EDIT: Yes, I just checked and it will grab what is in the buffer if the tuner it uses is on the same channel.


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Improving Your Business Processes & Project Management
April 23–24, 2014 | 9 a.m.–4 p.m. daily
Every business must constantly improve their processes to be successful. This seminar covers the basics of Business Process Improvement including: terminology, identifying and selecting projects, planning, management and execution, analysis and measurement. This session includes practical exercises and examples that can apply to any type of business, large or small. Participants may enroll in the first day of Improving your Business Processes or both days which includes the Project Management session.
The seminar's objective is to provide participants with the basic tools to improve their businesses in meaningful ways. The result is lower costs, fewer errors, more efficient use of resources, better customer service and more engaged employees from applying these concepts to their business processes.
Participants will be able to develop and successfully execute Business Process Improvement projects. You will learn basic project management and process mapping, including techniques and tools. You will also learn how to measure the results of work and how to monitor and adjust new processes. This seminar is presented by the OSU Department of Management and the Center for Executive and Professional Development.
The one-day process improvement seminar paired with the one-day project management and practical application seminar is a powerful combination to take back to your organization and apply this information to your workplace.
Attendees will be better able to:
- Understand your business processes and how they affect organizational performance
- Identify areas to improve in your organization
- Organize and plan a process improvement project
- Use process mapping tools and techniques
- Analyze and improve your current-state processes
- Test and apply changes
Participants who are…
- Project Leaders
- Directors
- Managers
- Supervisors
Marc Tower, is an adjunct instructor for the Department of Management in the Spears School of Business at Oklahoma State University. He graduated from OSU with an M.S. in Electronic Engineering Technology and an MBA from the Spears School of Business.
From being a scout pilot for the US Army to spending the last nine years helping companies operate more effectively, Marc has always worked toward operating efficiently and effectively. Having worked in the military, engineering, commercial software, construction and financial services, he has a broad experience background that helps show others how to apply Business Process Improvement principles to any situation.
For the last nine years Marc has been a consultant and president of First Ascent Consulting, LLC. For the last two years, he has consulted for firms ranging in size from very small to more than 10,000 employees.
April 23, 2014: IMPROVING YOUR BUSINESS PROCESSES
Process Improvement Introduction
- Why process improvement is important
- Defining a process
Where to Start?
- How to select a project
- Goals, scope and management
Basic concepts
- Time
- Value
- Inertia
Diagramming processes
- Tools
- Exercises
- Teams
Measuring Outcomes
- What to measure
- How to measure
- Analysis and Application
- Applying Changes
- Measure and Adjust
- Closing discussion
April 24, 2014: PROJECT MANAGEMENT—FURTHER IMPLEMENTATION
Project Management for Process Improvement Projects
- Project Structure
- Communication, communication, communication
Advanced Diagramming Options and Timelines
- Available tools-comparison
- Data collection techniques
- Time studies-when and why
Practical Application – Attendee projects or more complex exercises
- Individual or teams, structuring and beginning project for your organization
- $395–Enrollment for day one, Process Improvement, April 23, 2014.
- $695–Enrollment for day one and day two, Project Management & Practical Applications, April 24, 2014.
For multiple enrollments of 3 or more, registration is $585 for days one and two, per person.
Registration includes continental breakfast and break refreshments.
Center for Executive and Professional Development | Spears School of Business | Oklahoma State University
Phone: 405-744-5208 | Toll Free: 1-866-678-3933 | E-mail: email@example.com
On-site program – This program can also be offered on-site at your organization. If you have a group of individuals or team who you believe this program would benefit, please contact Ms. Courtney Garrison at 405-744-5208 or the OSU Center for Executive and Professional Development at 1-866-678-3933.


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AIPAC: so 2008?
- By Annie LowreyAnnie Lowrey is assistant editor at FP.
Here at FP, we watched U.S. Vice President Joe Biden and Sen. John Kerry's speeches at the annual American Israel Public Affairs Committee conference. We parsed the response to the two-state solution proffered by both. We considered their call for a settlement-freeze. We read about Netanyahu's reception.
Israel and Palestine — and AIPAC especially — tend to be tinder-box issues, and we expected to find ourselves amid some policy or defense discussion (flamewar?) in the blogosphere.
Oddly, what we noticed most was the sound of crickets.
Why the silence? Barack Obama isn't speaking (the big reason for last year's spike), The Israel Lobby is old news, there's relatively little violence between Israel and its neighbors, and the Israeli elections are over; plus, no one has yet said anything too contentious. The issues that make AIPAC's conference hot are, for the moment, relatively cool.
An extremely unofficial measurement of the response — Google Trends — seems to bear the observation out.


text:
My Boba Fett helmet just came in the mail! Can't wait to get started on it
It doesn't say whether it's a Don Post or Rubies but, it says 1997 Lucasfilm. A '97 would make it a Rubies right? . In any case, I purchased this on ebay for $30 and I'm going to use it as a starter helmet until I got the funds to get a BobaMaker.
Has anyone else had or worked on a 97 helmet?
When I get home I'll take some pics of it...then pics of my progress
So, here are some pics...Although everyone here may know already what it looks like...I just wanted some "before" pics...Plus I just wanted to show the shape it's in...its warped in a few places


text:
Property: Etrusconia Bed and BreakfastAddress: Via W. della Gherardesca 2, 57022 Marina di Castagneto Carducci, Castagneto Carducci, Italy
Location: Italy > Tuscany > Province of Livorno > Castagneto Carducci > Marina di Castagneto Carducci
Business Center with Internet Access Free Breakfast Free Parking Pets Allowed ( Dog / Pet Friendly )
Ranked #1 of 1 B&Bs / Inns in Marina di Castagneto Carducci
Price Range (Based on Average Rates): $
Hotel Class:1 star — Etrusconia Bed and Breakfast 1*
Number of rooms: 10
- Official Description (provided by the hotel):
- It lies on the coast with large private beach and parking, large garden, air conditioning, TV.Offers a service to reserve a large room with breakfast buffet and full board with support agreed restaurant at a fixed price.The hotel is ideal for holiday and business travelers will find a warm and casual atmosphere providing an ideal accommodation for a holiday immersed in the sea and pine forest on the coast of Etruscans. ... more less
- Reservation Options:
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text:
International Edition
May 27, 2017
About Us
Contact Us
Taishin Financial Holdings reports record-high Q1
By Ted Chen, The China Post Wednesday, May 14, 2014, 12:08 am TWN
TAIPEI, Taiwan -- At an earnings conference Taishin Financial Holdings Co. (台新金控) yesterday reported a record-high performance for the first quarter of this year, recording revenues of NT$4.82 billion, with earnings-per-share (EPS) reaching NT$0.61, showing an improvement of 13.95 percent year-on-year growth.
Throughout the first four months of this year, the company accumulated revenues of NT$2.23 billion, of which NT$1.32 billion in net profit can be attributed to the parent corporation, and yielded an earnings-per-share performance of NT$0.77.
Following the earnings conference, foreign institutional investors indicated that the company's EPS performance this year may reach a record-high NT$2.5.
Taishin Raises NT$6 Billion in New Capital
Most notably, in late April the company received NT$6 billion in capital raised in a round of 500 million newly issued common shares at NT$12, the rate approved earlier by regulators. The company also has plans to raise an additional NT$8 billion through subordinated corporate bonds. The company stated that newly raised funds will be used in improving its capital structure and to meet cash requirements when expanding toward markets abroad.
The company's board of directors announced that this year's dividend payout has been tentatively set at NT$0.42 for cash dividends and 0.99 for scrip dividends, pending the result of deliberations in the upcoming shareholders' meeting.
Meanwhile, in light of a protracted struggle regarding the proposed merger with Chang Hwa Bank (CHB, 彰化銀行), Welch Lin (林維俊), chief financial officer at Taishin Financials, yesterday stated that financial losses will be heavy should the government rule to have the company relinquish its stake in CHB.
Lin stated that, under equity recognition criteria, the company holds a 22.5-percent stake of CHB, which as of the end of last year is valued at a book value of about NT$26 per share, totaling about NT$45.4 billion.
While Lin declined to comment on CHB's upcoming board of directors elections, set to take place in six month's time, Lin stated that Taishin Financial was among the list of financial institutions invited by the government to invest and acquire stakes in the state-run bank. Lin added that the company is willing to cooperate with the government's suggestion, but urged for future policies to consider the interests of the company's 200,000 shareholders.
Promising writer dies at 26 from apparent suicide
Ruling puts Taiwan on verge of being first in Asia to have same-sex marriage
Terry Gou in apparent White House meeting with Trump
Shootout rattles New Taipei
The hidden cost of Taiwan's cramming culture
Cancer cases continue alarming rise
After nearly 70 years, Delta makes final flight from Taiwan
Writer's apparent suicide puts spotlight on rape
Stressed? Better lay off that fried chicken, ice cream, chocolate bars...
Not your ordinary KMT delegate


text:
I Am Borg
Or rather, I Have Joined The Twitter Collective. To be honest, I really wasn't sure of the point of Twitter until someone described it as 'microblogging' and then suddenly it made sense to me. I don't actually post that frequently, but there are often little scraps and bits of pieces of information I stumble across here and there that often have me thinking, 'oh, that looks interesting. Maybe I should blog about that', and then never do. But this way, a couple of words on something and maybe a link using Twitter seems like a good way to get past that.
Subscribe to: Post Comments (Atom)


text:
Indoor Activities
Explore Orange County Activities
From rare water creature encounters at the Aquarium of the Pacific to the historic vibe of Queen Mary, to sunset cruises, Huntington Beach offers exciting things to do for every families, couples and solo travelers.
Aquarium of the PacificCome face to face with hundreds of marine species, including sharks, sea lions and moon jellies, and learn about ocean exploration at this spectacular aquarium, also home to colorful birds at the Lorikeet Forest.
- Shark Lagoon
- Sea Otter Habitat
- Penguin Habitat
- Ocean Science Center
- Whales: Voices in the Sea
The Queen MaryRelive a bygone era as you tour the grandest ocean liner to sail the seas. View elegant staterooms, look for ghosts in the first class pool, and visit the Titanic exhibition. There is also a Cold War era submarine docked next door that is open to the public.
- Tours
- Attractions at night
- Restaurants
- Haunted Encounters
- Scorpion Submarine
Gondola CruisesThe beautiful waterways of Huntington Harbor are ideal for a romantic Gondola ride. Take in the stunning views of million dollar homes, magnificent yachts and enchanting scenery as you are guided along by an opera-singing gondolier.
- Stunning views
- Opera-singing gondoliers
- Complimentary photograph, blankets and stemware
- Welcome to bring own food and beverages
For more information visit Sunset Gondola
Professional SportsGet your adrenaline pumping and root for your favorite team at several professional sports venues within a short distance from our Huntington Beach hotel.
- Anaheim Stadium (baseball)
- Honda Center (hockey)
- Staples Center (basketball)
ShoppingWhether you prefer eclectic, one-of-a-kind pieces at the Old World Village galleries or the latest in fashion trends at the Fashion Island, discover an array of shopping venues to suit your individual style.
- Old World Village – imported gifts, art galleries, Bavarian authentic delis
- Fashion Island Newport Center – over 200 retailers and restaurants
- Downtown Huntington Beach – an exciting range of shops and restaurants with ocean breeze
Area Activities
Make a Reservation


text:
Now Foods, Coral Calcium, 1000 mg, 100 Veggie Caps
Now Foods, Coral Calcium, 1000 mg, 100 Veggie Caps
- You earn loyalty credit for every order you place at iHerb!
- Loyalty credit is equal to 5% of the value of your order, excluding shipping charges.
- Your loyalty credit will be applied towards your next order as soon as your current order has shipped.
- Your loyalty credits are valid for up to 60 days from the date of your last purchase.
Expiration Date:?Mar 2022Expiration Date
The length of time for the expiration date or "best used before" date depends on the type of product, as well as the brand.
Perishable items (such as flax oils or certain probiotics) generally have shorter expiration dates. Although our warehouse is fully air-conditioned, these more fragile items are put in cold storage (freezer or refrigeration unit) for maximum freshness.
Our receiving department does its best to verify and then enter the correct expiration dates for all incoming products. However, discrepancies do occur from time to time. This being said, the exceptionally high turnover at iHerb ensures that our inventory is among the freshest in the industry.
Shipping Weight:?0.47 lbsSwitch to Metric units
- Product Code: NOW-01273
- UPC Code: 733739012739
- Package Quantity: 100 Veggie Caps
2.5 x 2.5 x 4.4 in, 0.4 lbsSwitch to Metric units
|Combo Discount:
|- $4.00
- Healthy PH Balance
- Important for Bone Health
- 350 mg of Calcium
- GMP Quality Assured
- A Dietary Supplement
NOW Coral Calcium contains an alkaline form of Calcium that can help to support a healthy serum pH. In addition, Coral Calcium has naturally occurring trace minerals that are important for bone health, as well as for optimal enzymatic activity. NOW Coral Calcium is not harvested from living coral reefs or even from the ocean environment, but from above-ground sources in an ecologically friendly manner.
Suggested Use
As a dietary supplement, take 1 capsule 2 to 3 times daily, preferably before meals with water. Consider taking these other NOW products: Magnesium, OsteoBoron, Vitamin D and Zinc.
Other Ingredients
Cellulose powder, cellulose (capsule), silica and magnesium stearate (vegetable source).
Store in a cool, dry place after opening.
Caution: For adults only. Consult physician if pregnant/nursing, taking medication, or have a medical condition. Keep out of reach of children.
Do not eat freshness packet. Keep in bottle.
|Supplement Facts
|Serving Size: 1 Veg Capsule
|Amount Per Serving
|% Daily Value
|Calcium (from Fossilized Coral Calcium)
|350 mg
|Fossilized Coral Calcium
|1.0 g (1,000 mg)
|† Daily Value not established.
Good product
Verified Purchase
Good product
I have taken Coral Calcium for several years. Helps to keep your body alkaline.
Coral Calcium is the better of all the calciums.
Posted by 5643067431518685455 on Mar 06, 2017 | Verified Purchase
I believe that Coral Calcium is the better of all calcium particularly the Japanese kind. Most importantly it is alkaline and natural and not like the normal ones which is man made and acid.
Calcium bunch
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No need to drink milk or eat dairy products so much in order to get daily dose of Calcium.One cap gives me all what I need for the day.
New Favorite
Posted by SherryB on Aug 23, 2012 | Verified Purchase
Coral calcium is my new favorite calcium. I like the idea of trace minerals from the sea. So many items from the sea are good for balancing blood PH. Seems to work as well as some other calciums. Now products are one of my favorite product lines. No nonsense company. They don't use a lot of gimmicks are pretty labels. Just solid products.
Energy on the go!
Posted by herbalturtle on Jun 17, 2012 | Verified Purchase
Vegetarian capsules, great price and easy to digest calcium as well as lot of trace minerals which seems to give me energy. I'm all for that!
Works For Me
Posted by Reviewer2053243 on Aug 16, 2011 | Verified Purchase
I take 2 caps a day and find it works well.


text:
Nov 8, 2013 - By Bruce Tippets, Sports EditorAaron Nirider didn't have any problems adjusting to college golf at Eastern Wyoming College.
Nirider, a 2013 Riverton High School graduate, has stepped right into the golf lineup for the Eastern Lancers in Torrington.
The Lancers won four of their fall tournaments to sit in first place in the Region IX Division I.
"Aaron performed very well for his first college semester," Eastern Wyoming golf coach Casey Jones said.
Nirider is sitting third in the region individually. He finished in the top six in all five fall events and won Eastern's home tournament in Torrington.
Nirider won the title with a score of 71.
"Aaron is hitting the ball very well," Jones said. "He has improved his putting. If he continues to do so, he will be in position to stay right in the top three."
Golfers had to battle tough conditions in the tournament at Eastern.
"We got rained out of a couple of holes in Torrington, but were able to play 36 holes in the rest of the tournaments."
Jones has lofty expectations for Nirider the rest of his freshman season.
"I hope he continues his excellent play for himself, but more importantly for our team," Jones said.
In Nirider's third-place finish at Lamar, had two scores of 74 for a 148.
"We have a very good team," Jones said. "As talented as Aaron is, he is actually our No. 3 man."
The two sophomores on the roster for Eastern are Garrett Mortimer and Brandon Rideour.
Mortimer is from Casper, while Rideour is from Wamsutter.
"Two sophomores are leading the team, although Aaron did play a little better than one of them in a couple of tournaments. Also our four man is very similar to Aaron as far as ability and potential. We really have four good players and two more kids who are competing for the five-man spot with very solid scores and hard work."
The other first-year players are Chandler Meeboer (Casper), Josh Wilson (Plain City, Utah) and Deyten Klein (Holyoke, Colo).
"I really like this team because of their talent," Jones said.
"Their focus in the classroom, and how they are interacting with the community in Torrington. They are representing our college and their home towns very well so far."
The spring season for the Lancers start on March 27-28 at the Northeastern Junior College in Sterling, Colo.
There are a total of five spring tournaments on Eastern's schedule.
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Money Wars: Goldman Sachs vs. OWS
Posted on Oct 25, 2011
In this clip from Tuesday's "Democracy Now!" we hear the story of Goldman Sachs' recent move to back out of a fundraiser for the Lower East Side People's Federal Credit Union after the financial giant caught wind that the event would pay tribute to the Occupy Wall Street movement. But, as Amy Goodman and investigative reporter Greg Palast point out, there could be an ironic back story here about the $5,000 in pledge funds that Goldman also pointedly withdrew. Take a look below. —KA
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