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Title: Colleen Moore's Doll House: The Story of the Most Exquisite Toy in the World
Description: NY: Garden City Pub. l935. lst. b/w photo illus. Sm.4to. Wraps, lt.wear, else near Fine. ¶ An Illustrated description of this marvelous miniature castle.
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How vaporizing marijuana at different temperatures affects the vaporizing experience
How vaporizing marijuana at different temperatures affects the vaporizing experience
One of the major selling points of up-market vaporizers is their wide temperature range. The problem is, some people are confused by such a wide range and sweat over what temperature they should be using. To add to the confusion, there's lots of talk recently about how vaping at different temperatures either improves or dulls the experience.
The fact is that marijuana releases its essential oils and THC at temperatures between 175°C to 200°C, (that's 347°F to 392°F). Within this range of temperatures you'll get all the essential elements of your cannabis but none of the harmful toxins that are associated with smoking.
Unless you've been living in a different galaxy for the last thirty years or so you will know that smoking is harmful to health. Some folks are under the impression that is the substance, i.e. nicotine, that is dangerous, but the truth is, it is the burning that produces most of the toxins. This means that if you burn marijuana you are producing many of the toxins that injure or kill tobacco smokers.
If you set your vaporizer temperature at the high end of the range, you are very close to burning it; the vapor might taste a little smoky and your high is likely to feel harsh. Vaping at the higher temperatures produces a thick vapor that is not dissimilar to smoke. At these high temperatures, you will also destroy cannabidiol (CBD), which makes up 40% of marijuana's active constituents.
CBD has sedative qualities, and it slows down the rate THC clears from the body; so your high won't last as long. CBD will not last as long. CBD is also very valuable from a medical point of view, especially as a pain killer.
Turn the down the dial for the best experience. Take a look at your bud – if it's turning dark you are into the burning zone.
Something else to remember is that, by using a lower temperature, you are not destroying anything – just extracting the active ingredients. As not all those essential vapors are used up in one sitting you are actually getting more for your money.
By keeping your screen clean and not over packing your bud you should find that you get a far cleaner hit at the lower temperatures, and you will also get more for your money.
- October 7, 2015 at 1:47 pm
Glad to see somebody
Glad to see somebody wrote a guide to this, I do this at home with an Extreme Q and love it!
A few things for people with different setups than this:- For those with the Extreme Q, if you buy a second elbow piece(the part that attaches your hose to Cyclone Bowl , it comes in standard sizes and will fit perfectly, only costs 5$!)- It can take a few seconds of pulling to fully heat up the herb in allot of vapes. So, for those with bigger bongs you may find it better to pull really slowly, fill the bong to the top with vape, exhale while cupping the top, then take a full toke. Otherwise I end up with a big lung of air the first pull.

subset: commoncrawl


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Marijuana hater Jeff Sessions, a fierce drug warrior and former Senator from Alabama who is now President Donald Trump's attorney general, made news this week by threatening to attack the legalized marijuana industry. Now news of a different kind is threatening to get Sessions fired. Top Democrats such as Senator Elizabeth Warren are demanding that […]
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If you are presently working toward a bachelor's degree, completing a minor in Organizational Leadership along with your bachelor's degree requirements will enhance your career possibilities.
© 2017 Indiana University–Purdue University Fort Wayne (IPFW). All rights reserved. IPFW is an Equal Opportunity/Equal Access University.
If you have trouble accessing this page because of a disability, please email Human Resources and Office of Institutional Equity at email@example.com.


text: I am looking to sell my Jeep and get an 08-10 sti. I am completely new to these cars and the modding so i was wondering if you guys could tell me a few thing I should do first. What exhaust, intake, chip should I get? I want a loud exhaust (running no muffler on my jeep) and I am looking for decent performance gains. I've already searched but I still could use help.


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- Condition:
- Refurbished
- Item No.
- 281576
- Mfg Part No.
- 0100071820
Product Information for Garmin Nuvi 255W Automobile Navigator (Refurbished)
Highlights for Garmin Nuvi 255W Automobile Navigator (Refurbished)
- Display Screen:4.3
- Form Factor:Mountable
- Display Resolution:480 x 272
- Battery Life:4 Hour
Marketing Information
Go wide and get peace of mind with n�vi 255W. This affordable, widescreen navigator leads the way with voice-prompted turn-by-turn directions that speak street names and optional MSN Direct services to get you there on time and keep you informed. It's packed with millions of destinations and maps for North America.n�vi 255W comes ready to go right out of the box with preloaded City Navigator NT street maps, including a hefty points of interest (POIs) database with hotels, restaurants, fuel, ATMs and more. It even announces the name of exits and streets so you never have to take your eyes off the road. Simply touch the color screen to enter a destination, and n�vi takes you there with turn-by-turn voice directions, 2-D or 3-D maps and smooth map updates as you navigate. Its digital elevation maps show you shaded contours at higher zoom levels, giving you a big picture of the surrounding terrain.With HotFix satellite prediction, n�vi calculates your position faster to get you there quicker.n�vi 255W sports a sleek, slim design and fits comfortably in your pocket or purse. Its rechargeable lithium-ion battery makes it convenient for navigation by car or foot. With its "Where Am I?" emergency locator, you always know your location. Simply tap the screen to get your exact latitude and longitude coordinates, the nearest address and intersection, and the closest hospitals, police stations and fuel stations.Navigation is just the beginning. n�vi 255W includes many travel tools including JPEG picture viewer, world travel clock with time zones, currency converter, measurement converter, calculator and more. With photo navigation, you can download Google Panoramio pictures via our Garmin Connect Photos website and navigate to them.It also comes with Garmin Lock, an anti-theft feature. Enhance your travel experience with optional plug-in microSD cards such as Garmin Travel Guides for detailed data on attractions.
Battery Information
General Information
Physical Characteristics
Operating Systems:
Power Description
Technical Information
Display & Graphics


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The Chinese government issued a bitter denunciation of Australia's allowing the notorious terrorist, the Dalai Lama, to visit the country and meet with the Prime Minister. The Chinese claimed he was not a religious leader, but engaged in "splittest activities and destroying national unity."
China recently built a high speed railroad extension into Tibet and thousands of ethnic Chinese are moving into that region for jobs. Tibetan culture is under assault and religious leaders are harassed and prevented from carrying out their responsibilities. There is little doubt the Chinese government hopes over the coming twenty years that Tibet will be ethnically altered as non-Tibetans eventually become the majority.
The Chinese government is going to accomplish its goals, but still feels the need to attack the Dailai Lama as some sort of danger. On his visit to Australia, the Dalai Lama talked to a few political leaders who showed him some courtesy, but will not do anything that interferes with Australia's $52 billion trade exchange with China. Let's get real. Oh, the Dalai Lama did engage in some of his splittest activities while in Australia. He visited the zoo and had his picture taken with children.


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- Nicole S. Steed and Gregory H. Steed, Steamboat Springs, a boy.
- Sherry Marren and James Marren, Craig, a girl.
- Jessica Haptonstall and Jeremy Haptonstall, Craig, a girl.
- Brandy Kay Teters and Charles Leroy McMacken, Oak Creek, a girl.
- Alicia Noland and Jeremiah Noland, Craig, a girl.
- Candice Martin and Hiram Martin, Steamboat Springs, a girl.
- Allison Kane and Matt Kane, Steamboat Springs, a girl.
- Allison Kane and Matt Kane, Steamboat Springs, a boy.
- Kim Boyce and Kris Boyce, Steamboat Springs, a boy.
Couple events
- Johnson and Wirta Engagement
Colorado Springs woman dies on Rabbit Ears Pass
Husband the driver of semitrailer that rolled over Sunday night
A Colorado Springs woman died late Sunday night on Rabbit Ears Pass after the Safeway semitrailer she was riding in rolled over and burst into flames. Her husband was driving the vehicle.
Eyes on the Tigers
Hayden football gaining respect
Two weeks and two wins into the season, the Hayden High School football team still has something to prove.
Luke Graham: Lock 'em up
The last time the Buffalo Bills made the playoffs, I didn't have my driver's license, I was still trying to figure out girls and Bill Clinton was president.
The Record for Sept. 8
12:09 a.m. An officer was requested in the 1400 block of Conestoga Circle. A juvenile couple was reportedly arguing. Police turned the male over to his mother.
Briefs for Sept. 10
Today through Wednesday, the Colorado Open Space Alliance will hold its eighth annual conference at the Sheraton Steamboat Resort.
Trail designers seek Howelsen input
Four-season trail users have an opportunity to contribute to the vision for Howelsen Hill's future at a public meeting at 7 p.m. Monday at the Olympian Hall Fireplace Room.
New pool, slides taking shape
Hot Springs renovation aims for Christmas-week opening
The construction site at the Old Town Hot Springs is looking more and more like the final product. A mechanical room has been constructed, the walls of a new, curving activity pool are being built and two new waterslides are nearly complete.
Steamboat hangs onto top 10
SKI magazine reader's poll rates resort ninth, same as last year
When it comes to the SKI magazine annual reader's poll, it's a case of dejà vu for the Steamboat Ski Area.
High quality of care noted in Steamboat preschools
Three Steamboat area preschools have earned top ratings in state evaluations.
Pressing charges slide in
Mudslide property owner considering legal action
The property owner of land involved in a Thursday night mudslide does not believe the slide was caused solely by natural occurrences, and is considering legal action against both the Yampa Valley Electric Association and Routt County.
Monday Medical: You can cut the risk of stroke
There are many myths surrounding the topic of stroke. Many people believe stroke is unpreventable. This is false. Stroke is largely preventable by following healthy lifestyle habits and possibly using medical treatment.
Driver crashes into home
Accident causes no serious injuries
An allegedly drunken Steamboat Springs man drove his speeding car into the living room of a Walton Pond apartment early Sunday morning, injuring only himself and leading law enforcement on a brief chase that ended in his arrest.
Aging Well: Music to the ears
Upcoming concert an opportunity for seniors to reap auditory pleasures
Exaltation, triumph, grandeur. Most of us experience little of this in our everyday lives. Yet, Ira Dubinsky feels these and many other powerful emotions while listening to classical music.


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For current announcements, visit the SAP News Center»
SAP NEWSBYTE - SAP AG (NYSE: SAP) today announced that Sybase, an SAP company, has received the Open Compliance and Ethics Group (OCEG) 2012 GRC Achievement Award and Principled Performance Prize for implementing SAP governance, risk, and compliance (GRC) solutions. These recognitions underscore how SAP gives customers the ability to implement successful GRC programs, in addition to setting an example for best practices. OCEG, a nonprofit organization dedicated to improving GRC programs, presented the annual awards at the Compliance Week conference in Washington, D.C., on June 5.
Sybase finished in first place by also being selected for the Principled Performance Prize. OCEG recognized the company's multi-year undertaking to make risk management, regulatory compliance and safe, productive operations more efficient by utilizing SAP GRC solutions. The goal of the project was to create a framework that would help improve overall company performance by:
"Sybase's nomination was notable for the way in which the company outlined five years of continual improvement, applying guidance described in the OCEG Red Book to enable that improvement," said Brian Barnier, chair of the OCEG Achievement Awards evaluation panel and principal analyst at ValueBridge Advisors. "Most important was the focus on driving improved business performance led by integrity. Sybase's analyses demonstrated how they used risk management approaches to turn potentially adverse situations into opportunities."
"For the past five years, Sybase has worked to create company performance that is led by integrity," said John Chen, CEO, Sybase. "To achieve this, we needed internal audit capabilities that integrated GRC principles within existing management structures. We are honored OCEG has recognized our implementation of SAP GRC solutions. It underscores our commitment to supporting a collaborative approach to risk across business functions."
For additional information on Sybase's integration of SAP GRC solutions, watch "Sybase OCEG Nomination Video Featuring John Chen." For more information, visit the SAP Newsroom. Follow SAP on Twitter at @sapnews and @sapgrc.
Media Contacts:
Susan Miller, SAP, +1 (610) 661-9225, email@example.com, EDT
Scott Malinowski, SAP, +1 (978) 287-1793, firstname.lastname@example.com, EDT
Shannon McLoughlin, Burson-Marsteller, +1 (617) 912-5412, firstname.lastname@example.com, EDT


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The new Fraiche Café and Bistro in cluster Y of JLT launched the four-day offer, which ended yesterday, to support global charity Water Aid.
The 'Wipe Your Bill Clean for Water Aid' initiative saw
customers given the option of paying for their food as usual, or doing the dishes for a free meal and seeing their bill amount donated to the charity. Fraiche founder and managing director Yousef Al Barqawi said about 100 out of the estimated 450 people they'd had through their doors since Wednesday had swapped dirhams for dishes.
"Everyone's taken it in good spirits," said the 29-year-old Jordanian. "It's good to give something back." Customers relished being involved in the charity idea, which was backed by global firms Unilever and Vileda.
Fraiche mid-morning breakfaster Lisa Szatsznajder, from Australia, and her French friend Alison Lehr both gave
it the thumbs up. "Love it," said Szatsznajder. "It's not often a café will offer a free meal."
But when it comes to dishwashing, Al Barqawi said Dubai dwellers really need to step up to the plate.
"The standards of hygiene in restaurants are far higher than in the home," he said. "We had to rewash all the dishes – but it's about the fun aspect of people taking part."
Full News here – Dubai Latest Trusted Stories Feed
Get up to 75% discount in Dubai hotels!


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Item: SN096
For cellular vitality
Suitable for vegetarians
650 mg per tablet
650 mg 60 Tabs
Serving Size 1 Tablet
|Amount Per Serving
|% Daily Value
|40 mg
|Acetyl L-Carnitine HCl
|500 mg
|alpha-Lipoic Acid
|150 mg
†Daily Value not established.
Other ingredients: silicified microcrystalline cellulose, dibasic calcium phosphate, stearic acid, silica, and magnesium stearate.
Suggested Use: 1 tablet, 1 to 4 times daily.
Do not use if either tamper-evident seal is broken or missing. Keep out of the reach of children.
CAUTION: If you are pregnant, may become pregnant, breastfeeding, have any disorder of blood sugar metabolism (such as diabetes), or suffer from any seizure disorder (such as epilepsy), consult your physician before using this product.
.23 lbs
For cellular vitality
Suitable for vegetarians
650 mg per tablet
Source Naturals ACETYL L-CARNITINE & ALPHA-LIPOIC ACID contains two critical nutrients, acetyl L-carnitine HCI and alpha-lipoic acid, to support your vital metabolic functions. These compounds support the body's own system for the maintenance of cell function as we age. Suitable for vegetarians and hypoallergenic.
"With my personal 100% money-back satisfaction guarantee, you have nothing to lose and great savings to gain."
Lee Swanson, Founder of Swanson Health Products
If you are dissatisfied for any reason, we'll give you a full refund and pay return shipping.
We are dedicated to offering the highest quality products at the best price. Find out more about how we ensure such a high standard in everything we do.Learn More
650 mg 60 Tabs
650 mg 120 Tabs
240 Tabs


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Anna Faris: Errands with Baby Jack
Taking her baby boy along for the ride, Anna Faris ran errands in Studio City, California on Tuesday (January 29).
The "House Bunny" star smiled sweetly in a black sweater, navy blue jacket, gray pants, and a black baseball cap as she toted baby Jack along in his carrier.
Soon, you can catch the 36-year-old actress in a new pilot for CBS called "MOM." Created by sitcom king Chuck Lorre, the comedy stars Ms. Faris as a newly sober single mother who's trying to get her life together in Napa Valley.
On Tuesday, it was announced that "West Wing" star Allison Janney will play Anna's character's mother in the show.


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ED Magedson – Founder
Ripoff Report
Gina's Cleaning Service2296 Allan Adale Rd. Melbourne, Florida USA
Gina's Cleaning Service cashed my check, failed to do the job, did not clean the house I paid her for Melbourne Florida
Does your business have a bad reputation?
Fix it the right way.
Corporate Advocacy Program™
I hired Gina's cleaning service to do a move out, deep cleaning after tenants left it filthy. She and I did a walk through and she said she would do the job. There is hardly any furniture there and she said this would make it easier to clean everything. She arrived very late, 6pm on a Sunday night. She texted me that it was completed a few hours later and that I would be pleased. I had left her a check which she cashed. I live in another town so the following weekend we went to inspect the house and the front door was ajar and unlocked. The front bay window blinds were wide open for anyone to look inside the living room.
The only thing semi-cleaned was the refrigerator. The place was still filthy. She said that the toilets are permanently stained. We soaked them in simple bleach and they are like new. When I texted her she said that a doll in the back room creeped her out and she asked the neighbor to move it. [continued below]....
This report was posted on Ripoff Report on 10/01/2013 09:54 AM and is a permanent record located here: http://www.ripoffreport.com/reports/ginas-cleaning-service/melbourne-florida-32935/ginas-cleaning-service-cashed-my-check-failed-to-do-the-job-did-not-clean-the-house-i-1088599. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
Ripoff Report has an exclusive license to this report. It may not be copied without the written permission of Ripoff Report. READ: Foreign websites steal our content
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*Photo courtesy of The Denver Post
Within hours of the start of the Cold Springs Fire Foothills United Way staff began supporting the response effort. Foothills United Way participates with Boulder County in the Emergency Operations Center [EOC] which is activated during a disaster. Our team was present in the EOC during the fire to bring coordination to financial contributions, donated goods and volunteering for the Cold Springs Fire. Government and voluntary organizations have to work together seamlessly to address needs in the midst of chaos, and Foothills United Way is trusted to take on that role.
The coordination between local government, voluntary and nonprofit agencies and local community leaders during a disaster is an integral part of response and recovery during a disaster. In early 2015 we helped form and currently provide leadership to the Boulder County VOAD (Voluntary Organizations Active in Disasters). This group has built relationships and systems that make local agencies effective in disasters. Over 20 nonprofit and faith-based organizations have been working together to help people affected by the Cold Springs Fire. BoCo VOAD agencies staffed shelters, offered emotional care, provided leadership for local community volunteers, helped those who are returning home after the evacuation, collected grocery gift cards for residents, cleaned up homes and properties, and performed ash-out for structures destroyed in the fire.
We have also partnered with 2-1-1 so that residents who have property damage from the fire can register their clean-up needs and get connected with a voluntary agency through the Boulder County VOAD. Through a platform called Crisis Cleanup we are able to effectively pair residents with volunteers who are eager to assist them.
Foothills United Way in partnership with BoCo VOAD members also launched the Mental Health Voucher program which provides up to $500 for individuals to meet with a mental health professional of their choosing. This program was created during the Fourmile Fire and used again during the floods of 2013. We recognize the importance of this program to an individual's recovery and resilience and are pleased to be able to offer it to those affected by the Cold Springs Fire.
We have also participated in a community meeting in August Nederland and will be present for a second meeting in October where we have been able to listen to residents needs, connect them with resources and learn about the unmet needs that we can help support with the Cold Springs Fire Recovery Fund.
An emergency response fund has been set up through Foothills United Way in collaboration with Boulder County for the collection of financial donations to help residents impacted by the Cold Springs Fire.


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Modular Building Directory
Illinois Modular Home Builders
Illinois Modular Home Company Profiles
Discovery Custom Homes (Division of Palm Harbor Homes)
15303 Dallas Pkwy, Addison, TX 75001
Genesis Homes
2701 Cambridge Court, Suite 220, Auburn Hills, MI 48326
All American Homes, LLC.
1418 S. 13th Street, Decatur, IN 46733
Commander Homes (Champion)
308 Sheridan Dr, Topeka, IN 46571
Commodore Homes of Indiana
1902 Century Drive, Goshen, IN 46526
Contempri Industries, Inc.
1000 W. Water Street, Pinckneyville, IL 62274
Crest Homes (Division of CMH Manufacturing, Inc.)
30 Industrial Park, Milton, PA 17847
Design Homes, Inc. of WI
Hwy 35 North, Prairie du Chien, WI 53821
Discovery Custom Homes (Division of Palm Harbor Homes)
15303 Dallas Pkwy, Addison, TX 75001
Dutch Housing
PO Box 258, La Grange, IN 46761
Fairmont Homes
502 South Oakland, Nappanee, IN 46550
Fleetwood Enterprises
3125 Myers St., Riverside, CA 92513
Franklin Homes
10655 Hwy 43, Russellville, AL 35653
Genesis Homes
2701 Cambridge Court, Suite 220, Auburn Hills, MI 48326
Giles Industries
405 S. Broad Street, New Tazewell, TN 37825
Hart Housing Group
1025 Waterford Street, Wakarusa, IN 46573
Heckaman Homes
2676 E. Market Street, Nappanee, IN 46550
Hi-Tech Housing, Inc.
19319 County Road 8, Bristol, IN 46507
Homeway Homes
100 Homeway Court, Deer Creek, IL 61733
Liberty Homes
1101 Eisenhower Dr. N., Goshen, IN 46527
Modular One, LLC
1884 Mines Road, Pulaski, TN 38478
New Era Building Systems
451 Southern Ave., Strattanville, PA 16258
Palm Harbor Homes
15303 Dallas Parkway, Addison, TX 75001
Redman Homes
308 Sheridan Drive, Topeka, IN 46571
Ritz-Craft Corporation
15 Industrial Park Road, Mifflinburg, PA 17844
Rochester Homes, Inc.
1345 North Lucas, Rochester, IN 46975
Stratford Homes Limited Partnership
402 South Weber Avenue, Stratford, WI 54484
Wisconsin Homes
425 West McMillan, Marshfield, WI 54449
Illinois (IL) Property Tax Notes : Taxes are imposed by local government taxing districts Most property in the state is assessed at 33.33% of its market value, except farmland which is based on its ability to produce income. There are several property tax relief programs.
Looking for more than Illinois Modular Home Builders?
- Indiana Modular Home Builders
- Iowa Modular Home Builders
- Kentucky Modular Home Builders
- Missouri Modular Home Builders
- Wisconsin Modular Home Builders
We strive to provide the best resource in your search for Illinois state modular homes. If there is a Illinois modular home builder that is not included in this directory please let us know. Our editors will be happy to research them and include them in this local modular home directory.


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Patchogue, N.Y. -- A storefront retail space in downtown Patchogue, New York, has secured leases totaling 7,400 sq. ft., according to North Plainfield, N.J.-based Levin Management.
The 23,000-sq.-ft. space at 35-71 South Ocean Ave. will feature sushi restaurant 360 Taiko Sushi & Lounge (4,900 sq. ft.) and apparel retailer Jeans Fashion (2,500 sq. ft.).
Patchogue, located on the South Shore of Long Island, N.Y., is undergoing a dramatic rebirth that is transforming the 2.2-square-mile village into a dining, arts and entertainment hub. New Village at Patchogue – a $100 million mixed-use development now under construction at the site of the former Swezey's department store – is enhancing the urban fabric of the town and promoting Patchogue as an active, pedestrian-friendly community.
35-71 South Ocean Ave. is located one-half block away from the New Village development, which will include more than 291 apartment units and hundreds of new residential tenants.


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Watch LIVE Video: Mavericks Surf Contest is a Go for January 2014
HALF MOON BAY, Calif. - To watch Live video of the competition, click here
Despite some hesitation, organizers for the Mavericks Invitational big wave surf contest say it will start this Friday.
Surfers from all over the world will be heading to the contest, which starts on Friday. Of the 24 contestants there are 8 locals from Santa Cruz county.
Earlier in the day, there was some doubt that the contest would be held Friday when event founder Jeff Clark posted this statement on Facebook, "Due to changes in the offshore weather the Call for the Mavericks Invitational will be postponed until noon today so we can see a favorable change. There is going to be SOUTH WIND and a GIANT swell. This combination can make it very dangerous, not only for competitors but for all boats out in the water. We will keep you posted."
To get tickets and more information go to mavericksinvitational.com


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About p3d
p3d is a general data-reduction tool intended to be used with data of fiber-fed integral field spectrographs (IFSs). The p3d spectrum viewer can be used with IFS data cubes of any origin. This tool can be useful for people who have access to astronomical data of such an instrument. Data-reduction tasks, which are performed by p3d, convert raw data of CCD detectors into extracted spectra that can thereafter be used for scientific purposes.
Version 2.4.2 was released 2017-04-13.
The aim with p3d is and has been to write a user-friendly tool that will reduce the time spent performing repetitive tasks in IFS data-reduction. p3d can combine several images (exposures) into one image, clean single images of cosmic-ray hits, can extract spectra using three different methods, does full error propagation through all steps, and provides graphical tools for inspection of both intermediate and final results. Release 2.4 of p3d contains tools to handle the following tasks:
- Create a master-bias image – prescan and overscan regions can, if available, easily be used instead.
- Clean raw data of cosmic-ray hits.
- Find and trace spectra on the detector, automatically.
- Create a dispersion mask for wavelength calibration.
- Create a dataset used to normalize (flat field) extracted spectra.
- Extract science-object data, use different extraction methods.
- Create a sensitivity function from a summed standard-star spectrum – this can be done either automatically or interactively; the sensitivity function is used to flux calibrate reduced data.
- Flux calibrate extracted data using a sensitivity function.
- Combine extracted images of separate detectors to one image (VIMOS).
- Correct data for effects of differential atmospheric refraction (DAR; PMAS/Larr, Spiral, VIMOS, FLAMES/Argus, and MPFS).
- Convert RSS-formated output of p3d to data cubes – for PMAS/Larr, Spiral, VIMOS, FLAMES, MPFS, and ERA2 – or the E3d-format (all instruments).
- Combine several extracted images using the same or different exposure times, where all exposures target the same region.
- Examine reduced spectrum images or IFS data cubes of any origin (such as MUSE) with the p3d spectrum viewer.
p3d also provides graphical tools to inspect raw data and outcome of the different tasks. Flux calibration can be done both with the routines of p3d, or with, for example, IRAF (instructions on necessary steps in this case are available at the documentation WIKI).
p3dTutorials are available for a few instrument setups on the Documentation & Tutorials page. The tutorials cover instrument setup, data reduction, and visualization.
Primary Reference
The primary reference regarding the functionality, the methods, and the implementation of p3d is:
"p3d: a general data-reduction tool for fiber-fed integral-field spectrographs", C. Sandin, T. Becker, M.M. Roth, J. Gerssen, A. Monreal-Ibero, P. Böhm, and P. Weilbacher 2010, Astronomy & Astrophysics, 515, pp.A35.
This is also the reference that should be used in publications that have made use of p3d for the data reduction. Additional references and links to those references are provided on the references page; please have a look at this page to see which other references you will want to cite.
If your research benefits from the use of p3d, we would appreciate the following acknowledgement in your paper: "This research has made use of the integral-field spectroscopy data-reduction tool p3d, which is provided by the Leibniz-Institut für Astrophysik Potsdam (AIP).".
In October 2010, at the STScI-workshop "IFUs in the era of JWST", Christer Sandin gave a presentation with the title "Integral-Field Spectroscopy Data Reduction Made Easy with p3d". Please, follow this link and watch the presentation.Software platform
p3d is written in the IDL language of EXELIS Visual Information Solutions. While p3d is licensed under GPLv3 and can be used freely with the help of provided tools, IDL is in itself a proprietary program.Supported instruments
We refer to p3d as a general tool since it has been developed to work with any fiber-fed integral field unit (IFU) of any IFS. Currently, the software has been configured and tested with the following instruments:
- PMAS – placed on the 3.5m telescope at the Calar Alto Observatory in southern Spain. p3d supports data of both the old 2kx4k CCD and the new 4kx4k CCD.
- The Mitchell Spectrograph [VIRUS-P] – placed on the 2.7m Harlan J. Smith telescope at the McDonald Observatory in western Texas.
- VIRUS-W – currently placed on the 2.7m Harlan J. Smith telescope at the McDonald Observatory in western Texas.
- SPIRAL and SAMI of the AAOMEGA spectrograph – placed on the 3.9m telescope at the Anglo Australian Observatory.
- VIMOS – placed on the third unit telescope (UT3) at the VLT in Chile. p3d supports data using the medium- and the high-resolution (MR and HR) gratings. Data sampled both before and after the instrument refurbish about October 2010 are supported. Data sampled using the new HR-blue setting will be supported as soon as we get a request for this (soon in any case).
- FLAMES – placed on the second unit telescope (UT2) at the VLT in Chile. p3d supports data using the ARGUS IFU as well as the two mini-IFU configurations. Data sampled both before and after the instrument refurbish in 2007 are supported.
- MPFS – placed on the 6m SAO Big Telescope Alt-azimuth (BTA) near Mount Pastukhov in the Caucasus Mountains in Russia. p3d has been setup to work with the newer CCD.
- GMOS-N and GMOS-S – placed on the Gemini North telescope on top of Mauna Kea on the Big Island of Hawaií, and the Gemini South telescope on top of Cerro Pachon in Chile; both telescopes belong to the Gemini Observatory. p3d supports data using the two one-slit modes; i.e. the red and the blue slits. The two-slit mode is (logistically) more complex to handle, which is why it is not (yet) supported.
- INTEGRAL, which is a set of IFUs connected to the WYFFOS spectrograph at the 4.2m William Herschel Telescope (WHT) (of the Isaac Newton Group of Telescopes) at the Roque de los Muchachos Observatory on the island of La Palma. p3d supports both the older single-detector as well as the newer two-detector configurations.
- ERA2, which is an life-sciences integral-field spectrograph in development at AIP.
- MUSE, The Multi Unit Spectroscopic Explorer is a second generation instrument placed on the fourth unit telescope (UT4) at the VLT in Chile. Use the p3d spectrum viewer to study and analyze reduced MUSE data cubes of any size (the amount of available RAM defines the limiting size on the cube).
A tool as complex as p3d is never finished. There are several parts of p3d, which could be extended and improved upon. Additional tools could be written in order to optimize the control and analysis of the outcome. p3d can also, if required, be configured for more instruments. If and how this will happen at large depends on the interest of the community. Please, tell us if there is some functionality missing, in your opinion, or if you need support for another instrument. We are scientists with limited resources, but we will do what we can to help you out. We do consider taking on people with a suitable expertise in our development team.
The p3d team
seventh version, for release 2.3: 2015-12-16
sixth version, for release 2.2: 2012-12-13
fifth version, for release 2.1.2: 2012-04-23
fourth version, for release 2.1: 2011-11-24
third version, for release 2.0: 2011-06-20
second version, for release 2.0a: 2011-01-26
first version: 2010-02-04


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The prettiest little box set containing minis of the best of Nourish's Radiance collection, rich in opulent Damask rose, for a naturally bright and blooming complexion.
Pores are refined and the complexion is smoothed and softened as radiance is enhanced.
Makes an ideal gift or introduction to the Nourish range.
- Radiance Purifying Cleanser - 50ml
- Radiance Refreshing Toning Mist - 50ml
- Radiance Brightening Moisturiser - 15ml
- Radiance Rejuvenating Peptide Serum - 15ml.


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Jan. 1, 1931 Conceived on New Year's Day by a Catholic couple practicing The Rhythm System. My father drank a lot and wouldn't take "NO!!!!" for an answer.
Oct. 1, 1931 Happy Birthday to me. Grew up in New York City, upper West Side, running the streets of Morningside Heights and hitching on trolleys and trucks up and down Broadway and Amsterdam Avenue. Great fun.
June 1948 Graduated Cardinal Hayes H. S. and went to work at the N.Y. Journal-American as a mail clerk.
Oct. 1950 Korea misbehaving. Turn 19 and join the air force.. Texas. Biloxi, Michigan, Alaska and California. 11 rank changes up and down. Honorable discharge after 4 years with 1 stripe, no Good Conduct medal and no re-enlistment talk. But man did I have fun.
Oct. 1954 Back to the old neighborhood and work at the Journal-American selling used car ads over the phone. Meet Marlene.
Nov. 1956 Off to California with Marlene. Get married and work various sales jobs, 3 years selling Yellow Pages advertising, about a year and a half at the L.A. Mirror-News, and brief stints here and there selling desert land, modeling courses, and aluminum siding before drifting into selling cars.
Jan. 1961 Start of "The Auto Years". I sold Pontiacs, Fords and VWs. I sold GTOs, and Mustangs for full pop when they were hot items, a satisfying feeling after dealing with chiselers and strokers day after da
Nov. 30 1962 Son, Patrick born.
July 30 1964 Son, Dennis born.
Oct. 1, 1964 Quit drinking alcohol. Wow!
June 1966 Move from L.A. to Newbury Park in Ventura County. Sheep graze next door to us.
June 1969 End of "The Auto Years". Burned out. Hair and beard growing.
June 1970 Goodbye suburbs. Bulldozers have replaced sheep. Markets, gas stations and convenience stores are popping up.
June 1972 Nixon looks like a lock. Goodbye, U.S.A. Hello Trinidad And Tobago. Lots of swimming, music and ganja. Beautiful nine months. Unable to get a work permit, we return.
March 1973 On to Vermont, where we run the kitchen at a rustic, private school for kids who are not living up to their parents' great expectations. Leave 2 years later due to "creeping preppiness". Go to work at a textile mill, 7 pm to 7 am shift. Noisy, but fun.
Dec. 1977 The mill closes two weeks before Christmas. California calling.
Jan 1978 Back in L.A. I enroll in Radio School, write intros for Gong Show contestants and wind up out in Lancaster at a country station and from there to KROQ in Pasadena doing brief news stories I call The Quick News and hosting a sunday morning call-in show with Marlene, called, Patrick And The Herb Lady.
March 1981 Need bread. Adios radio, on to the Limo Driver life. Lots of fun, good tips and smoke. Like all limo drivers I had a couple of screenplays and I wound up working on the Thicke Of The Night Show as a researcher where I did a few appearances.
1984-94 Writing short stories, TV pilots and screenplays while working other jobs.
Jan 1994-Dec 1995 Thanks to nepotism, I worked 2 years on The George Carlin Show writing two episodes during that time. George and I both learned to despise "community writing" with tv writers.
Oct. 1, 1996 Turn 65 and decide to retire in the Catskill mountains.
Aug, 1997 Settle in Saugerties / Woodstock area and start working on turning Highway 23, one of my unsold screenplays into a novel.
2000 and on Self-publish Highway 23, The Unrepentant in 2007. George got a chance to read it before he died in 2008 and loved it.
Oct. 1, 2012 Looking for "Quien F!#kin' Sabe?", my new book to roll off the presses. Unlike Highway 23, Quien F!#kin' Sabe? isn't a story. It's a collection of thoughts, essays, quotations and poems by fictional friends in my head, like Mister Romance, Allison Wonderland, 47811 Up Above, Dominic Vobiscum, Senator Argo Stropmore, Frank The Wanderer. Ms. Barker, Otto B. Ottohere and others. There are also questions (some with answers, some without) and Stoner Doodles included. No latrine should be without this fine book.


text:
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Fair attendance good despite poor weather
Not all of the numbers are in yet for this year's Eastern Montana Fair, but according to Gail Shaw, fair manager, the event was well attended considering the rainy weather.
"In general numbers, Wednesday the gate was up," Shaw said. "Thursday, the gate was down and the rodeo was down, too. Friday night from 7 to 11 the weather was good for the Lorrie Morgan concert, and it looks like that did pretty well. The Demolition Derby [on Saturday afternoon] was a couple thousand dollars back from what could have been. The conditions were quite hard."
Shaw added that according to the men who picked up the beverages after the fair, it looked as if the beer sales were down about 50 percent overall.
She said that due to the rain, the carnival started a little late on Saturday, running from around 2 to 11:30 p.m.
"They [the carnival employees] made a safety check at 1:30 p.m. to make sure the rides were safe," Shaw said. "At one point, they shut down the Zipper because that ride has belts and pulleys that can slip in wet weather. The carnival people really did a nice job."
Shaw said that numbers and information are still being gathered by fair workers. The gate fee for the fair was raised to $3 this year, and that has not been calculated yet.
"As far as money is concerned, it looks like we were just slightly behind last year," she said. "Overall, people really supported our fair."
To accomodate the popular Mighty Thomas Carnival, the fair was moved one day back, from Wednesday through Saturday, instead of the usual Thursday through Sunday.
Shaw expects the Wednesday through Saturday dates also will apply to next year's event.
Results from Saturday's Demolition Derby can be found on page 6 of today's edition. Results from the rodeo will be published as they become available.
An After the Fair Memories edition, chronicling the 4-H students and other fair participants, will be published at a later date.


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Documenting a tornado and its aftermath.
Compelling storytelling squeezed into a backpack.
Capturing Homo sapiens in its natural environment.
Bringing the reader into the process of political reporting.
Packaging news on the Web in new and engaging ways.
How one teen's chemistry lab led to an indictment from the DA
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Army Takes Over in a Coup in Congo Republic; Massamba-Debat Is Replaced by His Defense Minister
KINSHASA, the Congo, Aug. 3 (Reuters) -- The Army seized power in the neighboring Congo Republic today and President Alphonse Massamba-Debat was reported to have left Brazzaville, the capital.
August 04, 1968
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Thomas Wolfe Memorial Mr. Hill talked about the life and writings of Thomas Wolfe.
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text: 8/11/10 8/11/10 POPSUGAR Celebrity Rachel McAdams Photos of Rachel McAdams August 11, 2010 by Celebrity 0 Shares Chat with us on Facebook Messenger. Learn what's trending across POPSUGAR. View on One Page Photo 1 of 6 ADVERTISEMENT () Share This Link Copy ← Use Arrows Keys → 1 Photos of Rachel McAdams Previous Next Share this post Rachel McAdams


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Not Too Late for Some Root Veggie Planting
Hello, I have never planted root vegetables or a fall or winter crop. I plan to do so this year. However, I missed the window to plant carrots, beets, onions and garlic this spring. Is it too late if I do it this week? I live in Portland and it has been really hot this week! Thanks!- Multnomah County, Oregon
Thank you for your question to Ask an Expert.
Beets and carrots can be planted from now until mid-July by direct seeding. It is definitely too late to plant garlic, which is best planted in the fall and then harvested in July. For onions, you could buy a six-pack of transplants and plant them for harvest in September or so. Scallions could be sown directly at this time.
Please let me know if you have any further questions.
Weston Miller
Community and Urban Horticulture Faculty


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E-mail to(For more than one recipient, type addresses separated by commas.):
Your name:
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Subject :
www.tele.net.in : The Japanese government plans to sell up to 1.12 million shares in NTT, reducing its stake in the former telecom monopoly to 33.3 per cent, which is the minimum level of ownership the government is required to hold in the company.
500 Character Left


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Spotted: Pretty Little Rolleiflex in Pretty Little Liars!written by hlphuonglinh on 2012-08-30 #news
The beloved analogue child, Rolleiflex is spotted in the American teen drama mystery-thriller (woooo spooky!) series Pretty Little Liars. I was indulging in my favorite television guilty pleasure when this baby appeared on the screen!1 4 Share Tweet


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It looked like a potentially great find. Browsing eBay earlier this spring, David Maus came across a photo with the name "Cuppy" on the back. Knowing his baseball history, Maus knew the photo had to be George Cuppy, the pitcher who spent the 1890s as the number two starter for the Cleveland Spiders. The number one guy? Fella named Cy Young. Maus bought the photo for a … [Read more...]
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The Village (edition 2012)
by Nikita Lalwani
Work details
The Village: A Novel by Nikita Lalwani
No current Talk conversations about this book.
I'm seriously unsure between 3 and 4 stars. About half-way through I would've said 3 stars but I enjoyed the end part of the story and that brought it back up to 4 stars. The story of Ray, Nathan and Serena visiting an open prison in India appealed instantly and I loved the idea of the BBC Documentary and even more so with Ray's culture. It seemed like it could only be a perfect mix for me as a reader, however after having read 'Gifted' and giving it 3/5, I feel this was marginally better but only just.
I engaged with Ray and took a liking to her and she seemed at odds in the prison - liking and respecting their beliefs yet conflicted by own modern western values mixed with her own cultural beliefs. The novel focuses on her at all times with other characters taking the limelight throughout the unfolding story. Yet something didn't seem to hook me as much as it should and I can't put my finger on what. I think I'd expected more from the villagers but they never really stole the limelight and it always came back to Ray.
It was however the ending that boosted the star rating combined with how much I enjoyed the beginning. All in all, I'd recommend it more to someone who enjoys novels set in India rather than because of the story.
References to this work on external resources.
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A follow-up to the award-winning "Gifted" traces the efforts of a team of journalists to understand and document life in an experimental open prison where convicted murderers share their lives in a humble village, a site that becomes incresingly and dangerously subject to the dubious moral codes of its drama-seeking visitors.… (more)
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Downtown Kalamazoo boasts over 4,300 parking spaces, many of which are within close walking distance to the Kalamazoo State Theatre. Take advantage of free metered parking on Saturdays & Sundays, ample free 90-minute curbside, and multiple ramps.
For a map of downtown parking and rates, please visit Downtown Kalamazoo: Parking Info.
Paid parking is also available Day of Show near the State Theatre on Burdick St. where Cedar St. ends.


text: I will say that's pretty freaking amazing. However, to be fair, he did lift his feet higher than he actually elevated from the ground which shows more flexibility than hops. Watch his outstretched hands. They don't get that much higher from where they started. That said, it is amazing and he put together the perfect NFL scout attention grabbing ice breaker. Props.


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or add another card below
Our instant e gift card can be used at all four of the Grafton Group locations in Harvard Square. Grafton Street Pub & Grill, Russell House Tavern, Temple Bar and Park Restaurant and Bar.
Known for outdoor dining, a diverse menu and a great bar scene every night of the week, Grafton Street Pub and Grill has been a neighborhood institution for more than a decade. Grafton Street Pub & Grill is named after a lively cobblestoned street in Dublin�s Temple Bar shopping district, and is located at the entrance to Harvard Square.
An intimate neighborhood bistro located between Harvard and Porter Squares, Temple Bar serves smart, sophisticated American fare alongside an incredible cocktail list of classics and innovative concoctions.
In the heart of Harvard Square, Russell House Tavern elevates the local tavern experience serving seasonally-inspired, classic dishes from New England and beyond, as well as a distinctive bar program featuring craft beers, an all-American wine list and a carefully-curated selection of handcrafted cocktails.
Located in Harvard Square, PARK is a modern neighborhood restaurant/bar designed to suit today�s drinking and dining needs. A place to grab a quick after-work drink or bite, enjoy a leisurely dinner, linger over cocktails, or spend an entire night, PARK offers a variety of experiences that will feel unique depending on which of the four distinct spaces you�re seated. With an eclectic design aesthetic, PARK is stocked with rousing conversation pieces that range from counter-culture artwork from the 1960s to antique book-lined shelves and a trophy case brimming with myriad oddities. Not to be outdone, Executive Chef Mark Goldberg brings a spirited sense of interpretation to PARK�s New American menu of homey classics, which are served alongside an internationally-inspired collection of craft cocktails, beer and wine.


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Jul 08, 2011
Just sending PW love!! By the way..I LOVE your profile photo!!
Sending happy house vibes your way!!! :) Hope it all went well!
CHEERS!! Happy ~Toasting Tuesday~
You were featured in the 2011 PW Yearbook so I wanted to stop by and say CONGRATS!!! And cheers to your beautiful wedding :)
cheers to 9999 posts.... one more to go, EEEK..... I'm right behind you :):):) Early 10K congrats!!!!
You DO realize you're ONE post short of reaching your 10K milestone, right?!! I JUST reached mine today, so hopefully you'll pop on so we can celebrate together! ;)
Hope you're having an awesome Monday!
Mica xo


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Harvest, AL, USA < change city >
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Reception at Huntsville Museum of Art
4461 Maysville Rd Huntsville Wedding In April


text:
Other characteristics:
measurement, gas
ATIs B12 2-Wire Modular Gas Detector is a flexible component system providing a variety of options to meet individual gas detection and alarm requirements. Series B12 transmitters are loop-powered instruments that transmit a 4–20 mA signal linearly proportional to gas concentration.
The B12 detection systems consist of individual modules that can be located where required. Sensor/transmitters, located in the area where gas leakage or buildup might occur, provide the basic measurement for the system. Sensor transmitters are available in either NEMA 4X or explosion-proof versions.
Transmitters are normally supplied with the sensor close coupled to the enclosure. However, for special applications, the unit can be supplied with separate sensors that can be located up to 25 feet from the transmitter. Sensor duct mounting adapters and sensor flowcells can also be provided for specific applications.


text:
Eugene, OR
Develops and maintains a broad knowledge of Company products and services while acting as an ambassador and representative within an assigned geographic territory consisting of Central Oregon. Establish and continue strong business relationships with prospects, clients and vendors.
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text: Ok so i finished the install sunday and everything seems to be flawless. i tried tuning the amp with a DMM. im running a 600W rms amp @ 2ohms to the 2 Subs (300RMS ea.) so the calculation should be 600 * 2 = 1200 (sqrt of 1200 = 34.64) so i tried tuning the gain to get 34.64v on the DMM.. does this sound right? this is what i did i had all the settings on the stock head unit at 0 (bass, trebble, fade..etc.) and the remote base boost control on 0db, and gain set down and freq high and had a 50hz/0db cd playing to test with the DMM.. got it to as close to 34.64 as possible without going over.. anything sound wrong? ( the subs wires were disconnected from the amp and the DMM was inserted in their place.) i have the amp hooked to the back speakers so when i use the 50hz/0db CD i can hear it playing.. which im not sure is good for my stock speakers but i have no other way of doing it since its getting the signal from the back speakers.


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Population Systems
€ 38,99
Sofort lieferbar
April 2008
BeschreibungThis unique book is concerned with the general principles and theories of population ecology, based on the idea that the rules governing the dynamics of populations are relatively simple, and that the rich behavior we observe in nature is a consequence of the structure of the system rather than of the complexity of the underlying rules. From this perspective, the dynamic behavior of single-species populations is examined and an elementary feedback model of the population system is developed. This single-species model is refined and generalized by examining the mechanisms of population regulation.
InhaltsverzeichnisContents. Preface to the first edition. Preface to the second edition. Acknowledgements. Part I. Population Systems. Introduction to Part I. 1. A Brief Look at General Systems. 1.1. What is a System? 1.2. The State of a System. 1.3. Dynamic Systems. 1.4. System Diagrams. 1.5. Feedback Control. 1.6. The Stability of Systems. 1.7. Anticipatory Feedforward. 1.8. Systems Analysis in Biology. 1.9. Chapter Summary. Exercises. Notes. 2. Population Dynamics and an Elementary Model. 2.1. What is a Population? 2.2. Dynamics in Populations. 2.3. An Elementary Population Model. 2.4. Analysis of the Model. 2.5. Environmental and Genetic Effects. 2.6. Chapter Summary. Exercises. Notes. 3. Population Regulation and a General Model. 3.1. Density-Dependant Mechanisms. 3.1.1. Competitive Processes. 3.1.2. Cooperative Processes. 3.2. Feedback Integration. 3.3. A General Population Model. 3.4. Analysis of the Model. 3.4.1. Environmental and Genetic Effects. 3.5. Populations in Changing Environments. 3.5.1. Environmental Feedback. 3.6. Complex Density-Dependant Relationships. 3.7. Chapter Summary. Exercises. Notes. Part II. Systems of Interacting Populations. Introduction to Part II. 4. Interactions between Two Species. 4.1. Population Interactions. 4.2. Cooperative Interactions. 4.3. Competitive Interactions. 4.3.1. Nonlinear Competitive Interactions. 4.3.2. Competition in Variable Environments. 4.3.3. Strategies of the Competitor. 4.4. Predator-Prey Interactions. 4.4.1. Nonlinear Predator-Prey Interactions. 4.4.2. Predator Functional Responses. 4.4.3. Predation in Variable Environments. 4.4.4. Predator and Prey Strategies. 4.5. Chapter Summary. Exercises. Notes. 5. Interactions in Space. 5.1. Introduction. 5.2. Movements in Space. 5.3. Dynamics in Space. 5.4. The Spread and Collapse of Pest Epidemics. 5.5. Stability in Space. 5.6. Population Quality in Space. 5.7. Environmental Stratification. 5.8. Chapter Summary. Notes. 6. Interactions between many Species (Ecological Communities). 6.1. Community Structure. 6.2. Community Stability. 6.2.1. Predation as a Stabilizing Influence. 6.3. Community Dynamics. 6.4. Chapter Summary. Exercises. Notes. Epilogue: The Human Dilemma. Answers to Exercises. Author Index. Subject Index.
PortraitPavel Kindlmann is professor of ecology at the Charles University in Prague, Czech Republic and performs research on various aspects of biodiversity as the Head of the Biodiversity Research Centre, in the CzechGlobe - Global Change Research Centre, Academy of Sciences, in eské Bud jovice, Czech Republic. His main interests include population and metapopulation dynamics and life history strategies with a special emphasis on insect herbivores, their predators, and on the orchid family. He authored or edited several books on population systems, orchids and conservation biology and published about 100 scientific papers in prominent journals.
PressestimmenFrom the reviews of the second edition:
"This text aims to build a concept of models and thus show how the more complex ideas can be understood. ... This is a detailed, technical publication which would be best suited to students of theoretical ecology. ... the gradual building of the model means that a wide range of readers will gain something from the text. ... Overall, a good text for the specialist and a useful reference for those really wanting to understand the theory of modelling from the ground up." (Thermoelectric News, July, 2008)
"Berryman (Washington State Univ.) and Kindlmann (Academy of Sciences of the Czech Republic) address the general principles and theories of population ecology using simplified interpretations of general systems theory and graphical procedures. ... Summing Up: Recommended. Upper-division undergraduate through research and faculty collections." (R. L. Smith, Choice, Vol. 46 (4), December, 2008)
Untertitel: A General Introduction. 2nd ed. Sprache: Englisch.
Verlag: Springer-Verlag GmbH
Erscheinungsdatum: April 2008
Seitenanzahl: XVI
Format: kartoniert


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Systems Biology Opens New Research Pathways
Systems Biologists Are Attacking the Encrypted Messages That Would Allow Us to Predict and Change the Course of Disease
Individual Tumor Profiling
Has Tumor Molecular Profiling Enabled More Effective and Less Toxic Cancer Treatment?
Top 10 Under 40
Up-and-Coming Stars Shine in Biopharma Research and Business
Podcast: FDA's New Commissioner Hits the Ground Running
Scott Gottlieb, M.D., Commits to Speeding Up Drug Reviews, and Maintaining Safety and Efficacy
Amplifying Immunoassay Sensitivity
Microfluidics-Based Microplate Technology Offers Benefits Over Traditional ELISA Methods
Click Image To Enlarge +Figure 1. Microfluidics microplate showing enlarged view of a microchannel associated with its microplate well
Enzyme-linked immunosorbent assays (ELISA) are commonly used in research and clinical applications to quantify analytes from complex matrices such as serum and plasma/cell lysates. Excellent selectivity is provided by the use of a high affinity capture antibody and multiple wash steps. Good sensitivity is achieved through direct labeling of an antibody to a different epitope on the analyte or the use of a labeled secondary antibody. The basic ELISA workflow and sensitivity have not changed significantly in decades.
Siloam's Optimiser microplate offers improved ELISA workflows, sample and reagent conservation, improved reaction kinetics, and the ability to fine-tune assay sensitivity through multiple analyte loading in fluorescent-based assays. The Optimiser uses an SBS-configured 96-well microplate architecture and a dedicated microfluidic channel immediately below each well (Figure 1) where binding occurs.
Each 200 x 200 μm spiral microchannel offers approximately 50 times larger surface area-to-volume ratio for capture of antibodies compared to a standard ELISA microplate, which relies on the microplate well bottom for capture. This allows for more efficient capture antibody binding and highly efficient antibody-antigen reactions, which decrease overall assay time.
Principle of Operation
The Optimiser workflow (Figure 2) mirrors standard ELISA assay steps, however, the volumes used are significantly smaller, and washing steps are reduced.
Sample/reagent volumes as small as 5–10 μL are added to each well and drawn through the microchannel via capillary forces. Each microchannel, where binding occurs, holds 4.5 μL of liquid, and excess liquid is channeled to an absorbent pad under the microplate.
The sequence of reagent/sample additions is demonstrated in Figure 2. For each successive addition, the capillary barrier is broken at the microchannel inlet, and all previous reagents are flushed into the absorbent pad. Flushing excess liquid effectively removes unbound materials and also prevents cross-contamination of reagents within the microchannel. By making multiple additions of sample containing analyte, sensitivity can be tuned up to 100-fold higher than single additions of analyte.
Automating the process further enhances ELISA assay efficiency and increases throughput. An automated pipetting station, such as BioTek's Precision™ Microplate Pipetting System, automatically loads analytes and reagents into the microfluidics microplate, and the multistation platform allows operation of multiple Optimiser microplates. Automation increases pipetting precision compared to manual methods, especially at the low volumes used in the Optimiser workflow, and also allows the user to attend to other tasks while the instrument is in operation.
As the Optimiser plate conforms to SBS recommended microplate specifications, it can be read in any standard fluorescence microplate reader.
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Amazon (AMZN) shares climbed nearly 4 percent today to close at an all-time high after a record holiday season for online shopping and praise from Wall Street for the company's global reach.
The company's stock ended the trading day at $268.46, the highest closing price (on a split-adjusted basis) since Amazon debuted on the NASDAQ in May 1997 at an initial asking price of $16.00. The stock split three times during the first dot-com bubble before languishing for years in the low-to-middouble digits for years following the bust.
But the stock has been on a tear since the 2008 crash as the company cemented its dominance in e-commerce. Over the past 12 months Amazon shares rose almost 50 percent and the upward trend seems to be continuing. Online holiday spending this past year hit $42.3 billion, a 14 percent increase from 2011, according to comScore. Amazon hasn't revealed its own holiday sales figures but called the season its "biggest ever." On its peak day, Amazon sold 26.5 million items, or 306 items per second, the company said.
Despite these numbers, credit for Monday's spike is going mainly to an upgrade from an internet stock analyst who raised his target for Amazon shares to $325. Morgan Stanley's Scott Devitt said in a note to investors that Amazon is so far ahead in global order-fulfillment infrastructure that other online retailers may never even get a chance to break into some markets.
Devitt also said he expects global e-commerce sales to nearly double by 2016 to $1 trillion, of which almost almost one-quarter would belong to Amazon.
Much of that dominance in the future will depend, Devitt suggests, on Amazon's vaunted logistical operations. This makes sense, since it's also key to Amazon's dominance today. Anyone can open an internet storefront these days. But few other companies can open 1-million-square-foot distribution centers or hire 50,000 seasonal employees to make sure orders get there by Christmas. No matter how good the website or the app, in shopping it's the ability to deliver offline that counts the most.Go Back to Top. Skip To: Start of Article.


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Well, just to preface this, I raced an 05 s4 4.2v8 from a stop the other day. I happen to be friends with the kid, well anyway, I destroyed him from a stop, he can't launch though. From a roll he pulled about a length to a length in a half from a third gear roll. Anyway, a girl friend of mine has an '06 mustang gt covertible (automatic), and she says she is going to smoke me. Well, I'm worried this is all too true, I know I can kill her on the backroads, but straight line performance. Would I get killed by her mustang, since its an auto and a convertible? Anyway, if i would get killed, would an AP stage I put me even with her?
-my only mod is my catback exhaust


text: I think it looks pretty good. Is that a sweatguard peeking out from behind the Glock? That would be a nice addition. You might consider a lighter background for the photos to get a better contrast. The clips and dark portions disappear on the black background. No recommendations for the dye; I don't work with leather.


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The nation of Qatar is rather quaint. It is ruled by a small elite which holds all power and it imports labor to handle the labor required in this small–but Wealthy– nation. The UN International labor Organisation blasted Qatar for its use of this imported labor force. This tiny land currently has 1.2 million immigrants who do all the grunt work. Qatar is host to the World Cup in 2022 and is building and building with the labor of those who never were born in Qatar. The ILO reports hundreds of examples of abused workers laboring under horrendous conditions. Safety rules are ignored, men work long hours with limited safety protection and they are treated –as immigrant labor. They are expected to work and work, receive low wages and allow Qatar to host a world athletic event.
The ILO found evidence of workplace accidents, men dying of access to sufficient water, many not even paid and the government taking away passports in order to prevent men from leaving the country. We will ignore the rape of domestic servants. Anyway, welcome in 2022 to a world sporting event!
Oh, anyone interested in me becoming your "guardian?" I will sponsor you, just pay me $3,500 for the right to have me as your sponsor.


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Spring Frenzy & Engineering Expo
Friday, April 29
The Trine University community invites you to visit during one of our open houses. Held once a month, you have the opportunity to learn important information about admission and financial aid, student life and housing, and the Employment Resource Center.
You will also have the chance to hear from our students, talk with professors, and join us for lunch and a campus tour. Click for full schedule.
Call today to reserve your spot, 1.800.347.4878 or fill out the form below:
Visit Day Registration (all fields required)
Upcoming Visit Day Open House
Spring Frenzy & Engineering Design Expo - Friday, April 29
7:30 Check-in & Refreshments – T. Furth Center for Performing Arts
8:00 Welcome
8:30 Interactive Panels
Student Life, Housing, and Campus Safety
Employment Resource Center & Student Success
9:30 Athletics, Music, and Student Activities Panels
10:15 Admission, Financial Aid, and Scholarships
10:45 Academic Breakout Sessions
11:45 Lunch
12:30 Campus Tours
1:30 Final Closing
2:00 Engineering Design EXPO