text:
WORLD OF DEATH Ep. 82 – The Silent
Finnish Director Toni Tikkanen's unmistakably eerie short, The Silent, is an exercise in how to effectively build tension and suspense by...
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text: In letters to his wife Nora, James Joyce addresses her as "my naughty little fuckbird," "my little cuntie," and "my sweet little dirty farter." And that's the G-rated stuff. Tomorrow night, Steve Almond (who else?) leads a reading of Joyce's filthy letters with a team of local writers and musicians; Hallelujah the Hills frontman Ryan Walsh, Hands and Knees, and the Juliet Kilo provide musical interludes to the literary raunch. That's at Great Scott, 1222 Comm Ave, in Allston.

subset: commoncrawl


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Pankaj Awasthi has been making music for the past 15 years, but he calls himself an "illiterate musician". He has numerous ad jingles and two folk-electronica albums to his credit but, by his own admission, barely knows much beyond the basics and can't read or write music. "I don't know all the fancy theoretical stuff," says the 38-year-old, with a laugh. "However, if you play a groove for me, I can come up with at least three or four melodies that go with it on the spot."
His latest album, Somrass (which roughly translates as 'a divine elixir of youth'), released about a month ago, but the official launch gig is on Sunday, in which Awasthi and his band will play tracks from the new album along with a few from his 2005 album, Nine. "The lyrics are very socio-political in nature," he says. "I tend to draw inspiration from Indian folk music and poetry."
Some diligent Bollywood listeners may identify Awasthi as the rustic voice behind songs such as Tera Hi Karam from Karam (2005) and Aaye mere saaye from New York (2009). However, Awasthi maintains a fairly low profile and admits that he "is terrible" at promoting himself. "I'd rather focus my energies on making music," he says. Somrass, an album which took three years to make, was made for the sake of music, not for commercial purposes. "It's a niche album, meant for those exposed to world music and poetry," he says. "You won't find the typical paagal-munda-soni-kudi kind of songs here."
Awasthi's career is a bit of a happy accident. At 20, he was a medical student studying at Manipal who had just picked up the guitar three years ago when he decided to drop out with a vague idea of pursuing music as a career. "My parents were, naturally, disappointed," he says. "They thought I had no direction." Within a few months, however, Awasthi started working with Susmit Sen, guitarist of folk-fusion band Indian Ocean, as a programmer at his studio. "I will always be grateful to him [Sen] for giving me a chance," says Awasthi.
While jingles and film scores earn him his living, Awasthi is content with making music for the sake of it as far as albums are concerned. "I don't need a lot of money to be happy," he says.
- Suprateek Chatterjee


text:
The U.S. Department of Energy selected a contractor Tuesday to oversee cleanup operations at the Paducah Gaseous Diffusion Plant, long seen as the first step in helping many of those laid off by the plant's closing get back to work.
The DOE selected Fluor Federal Services Inc., a global engineering, procurement and construction firm headquartered in Irving, Texas, for deactivation activities at the plant, currently leased to the United States Enrichment Corp. The estimated value of the contract is $420 million over three years.
According to the DOE, the award will support transitioning and preparing the site for deactivation and decommissioning of the former uranium enrichment facilities. The contract provides "extensive and detailed hiring preferences for the available, highly skilled labor force," the DOE said.
Reaction to the announcement late Tuesday afternoon was swift from local, state and federal officials.
In a joint statement released by Paducah Economic Development, Mayor Gayle Kaler, McCracken County Judge-Executive Van Newberry, Paducah Area Chamber of Commerce President Sandra Wilson and PED President Chad Chancellor said: "It is an extremely important action by the DOE for our community. The cleanup of the site will require decades to accomplish and cost billions of dollars. This contract will be the vehicle to begin this important work.
"The protection and safety of our community as the DOE begins to dismantle this huge complex and remediate the environmental impacts from 60-plus years of operations is of paramount concern to all. Our community is committed to maintaining its partnership role with the DOE, the state, our congressional delegation, and Fluor to accomplish this work."
Gov. Steve Beshear said, "This is good news for all of Kentucky, the Paducah region in particular ... I am very pleased the federal government is moving on this important project."
U.S. Sens. Mitch McConnell and Rand Paul and Congressman Ed Whitfield said, "Last year we fought hard to secure funding for the deactivation of the gaseous diffusion plant and environmental cleanup at the Paducah site and since then have pushed DOE to expedite this contract so vital cleanup work may begin. We are pleased that DOE has responded by awarding the contract for deactivation, which we hope will create job opportunities in the community."
Services to be provided under the contract include project management, facility modification and infrastructure optimization, decontamination and demolition, and environmental services. The plant is located on a 3,423-acre federal reservation approximately 10 miles west of Paducah.
"This gives us an opportunity to create employment for workers who just lost their jobs," said Charlie Martin, Paducah Area Community Reuse Organization executive director. "They (laid-off workers) are highly trained, working in the plant for decades. They know how to safely and efficiently perform the cleanup work."
The DOE had narrowed the field of contractors to two, Fluor and AECOM, either of which Martin said is highly qualified.
"We look forward to discussions with Fluor as they roll out the transition, mobilize and commence work," Martin said. "My understanding is they want to move as fast as they can."
Martin said the contractor will likely be ready to move soon, perhaps in a matter of days, not weeks or months.
"I certainly hope so," he said. "This is a significant event for the community, something everybody has put a lot of effort into. It's something that's been on everyone's mind."
Contact David Zoeller, a Paducah Sun staff writer, at 270-575-8676.
Check out these recently discussed stories and voice your opinion...


text:
Hey!! almost a year without any news from this blog, sorry sorry!! i've been expanding my knowledge about drawing and original characters. Yesterday was Lee's birthday and... i'm a little bit saddened for the lack of pictures for that fantastic day.
Also, I need to apologize for the little activity in the club.
I've noticed that there are a lot of Uke Lee drawings in DA, but the people didn't submit them to a club. Even if they're part of this club!!!! D':
Sometimes I can see them, but my DeviantWatch is always full! I have more than 1000 deviations in a week, Please, if you have a uke lee drawing, or you know a gorgeous picture of lee suggest to the group favorites or submit them. When i see them i can send invitations and expand the links.
Thanks for your time fellow Uke lee Fans!!!
WE ARE THE UKE LEE ARMY!! WE HAVE 100 MEMBERS!!! jojojojo


text:
The Composer's Advocate: A Radical Orthodoxy for Musicans
€ 23,99
Lieferbar innert 2 Wochen
September 1982
Beschreibung"An excellent piece of writing, exhibiting splendid communicative sensitivity and command of language, and fully representative of the remarkable mind of the author. . . . A book any musician, or any serious student or concertgoer, will find stimulating, provocative and instructive." -Robert C. Marsh, Chicago Sun-Times "Wise, detailed and valuable book. . . . Should become a set text for all performing artists."-Andrew Porter, The New Yorker "Throughout the book, Leinsdorf displays his knowledge and wisdom with a near total lack of pretense or vanity. This work would be considered one of the most significant books on conducting ever written, ranking in importance with those of Wagner and Weingartner. Its scope, however, suggests that it should be read not only by conductors but by ever practicing musician."-Robert Johnson, Music Library Association Notes
Untertitel: 'Radical Orthodoxy for Musician'. Revised. Sprache: Englisch.
Verlag: YALE UNIV PR
Erscheinungsdatum: September 1982
Seitenanzahl: 224 Seiten
Format: kartoniert


text:
Aretha Franklin, the undisputed Queen of Soul, was born on March 25, 1942 in Memphis, Tennessee. Raised in Buffalo and Detroit, she is the daughter of famous gospel preacher Reverend C. L. Franklin, pastor of Detroits New Bethel Baptist Church. She was greatly influenced as a child by family friends and gospel stars Reverend James Cleveland and Clara Ward.
Franklin first recording was in 1956 on the JVB/Battle label. She was signed to Columbia Records in 1960 by the legendary talent scout Jon Hammond. For Columbia she recorded Wont Be Long, Rock-A Bye Your Baby With Dixie Melody, I Surrender, Dear, Rough Lover, Dont Cry, Baby, Try a Little Tenderness, Trouble in Mind, Runnin Out of Fools and Cant You Just See Me. The nine recordings were all hit the Billboard pop charts, but none cracked the top 40.
It wasnt until Franklin signed with Atlantic Records in 1966, that her signature style was born under the influence of producer Jerry Wexler. During 1967 through 1977, Franklin had her greatest string of success with 15 top ten hits including I Never Loved a Man (The Way I Love You), Respect (#1, 1967), Baby I Love You, A Natural Woman (You Make Me Feel Like), Chain of Fools, (Sweet Sweet Baby) Since Youve Been Gone, Think, The House that Jack Built, I Say a Little Prayer, See Saw, Bridge Over Troubled Water, Spanish Harlem, Rock Steady, Day Dreaming, Until You Come Back to Me (Thats What Im Gonna Do), and Dont Play That Song.
Franklin signed with Arista in 1980 and reached the top ten again with Freeway of Love, a #3 hit in 1985. And in 1987, she had another #1 hit with a duet performance with George Michael called I Knew You Were Waiting (For Me).
The longevity of her career, versatility of her voice, and undeniable depth of her talent has made Aretha Franklin arguably one of the most distinctive interpreters of American popular song.
American Quartet


text:
Big Bass Marina
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
TripAdvisor Reviews Big Bass Marina Many
Travel Blogs from Many
Really Roughing It~!
... which was absolutely enchanting. We also saw some huge fish (which this area is famous for) but none were interested in our attempts to attract them to our lines.
Tomorrow is Father's Day and we are heading up the road to meet my sister and her husband in their rented motor home, for a mini family camping reunion at a lake in Arkansas. This will be very ...
Out of the Trace
... just happened to be posing, adding to the sense of luxury.
The Southernmost piece of the Trace is quite flat, but no less dreamy. The Cypress Swamp, a little north of Jackson on the Pearl River, is yet another place where you are urged to let go and float in the quiet of the uniquely Southern scenery. The location is spiritual, gothic even, with the grey cypresses and tupelos rising smoothly from their bowls in the silent water -- no branches until ...
Heading Off to Louisiana
... to pay, but that it wouldn't break his heart if we camped for free for the night.
The park really had potential to be a nice one with just a little maintenance. But, it wasn't. Weeds, poorly laid out, weeds, dust; just not real good. Luckily it was just one night.
Had a big helping of tuna mac for supper. No phone, No WiFi, No TV. Early to
Day 21
... turkey and Carla's famous stuffing! Never had deep fried turkey was very good! Boy, I have done alot of eating this past week. Not much happening today a lazy day! Jimmy, Carla and family were just wonderful this past week entertaining us...we are loving our time with them. And we will probably starve tomorrow as we are off to ...
Day 20
... is across the lake. Sat around and visited with all the camp folks....Jimmy made his famous ribs...wow cooked for 8 hours...fell off the bones. We had splash beans....found out today what there were. Someone spilled the beans, no pun intended....so they are now called splash beans. White, red and pinto beans mixed with spices very good. Sat and chatted at the campfire all evening...late night again. ...
Other places to stay in Many
Cypress Bend Resort, BW Premier Collection2000 Cypress Bend Pkwy, Many | 3 star hotelfrom $118
Starlite Motel160 Fisher Road, Many | 3 star hotelfrom $68


text: They look great. I got a 5 frame nuc and she is a very good laying queen. Lots of brood, capped and uncapped. Lots of pollen and honey. Just got them situated before it was too dark to see. A few buzzed out then flew right back into the hive. Curious girls. I'll feed them tomorrow since it was too late tonight and its going down to the 50's tonight. They were not happy with the 1 1/2 hour car ride. Just peeked in on them with my night vision monocular and a few of them are walking around the outside of the hive. I am excited to have them finally home.


text:
Serves 4
Parsley pesto
1 cup parsley
1 cup basil
1/2 cup flaked almonds
2 cloves garlic
Zest of one l small lemon
1/2 cup extra virgin olive oil, plus more to cook with
4 x 200g blue cod fillets
4 x 5cm slices of kamokamo
1 To make the pesto - put all the ingredients into a food processor then pulse until smooth. Taste for seasoning.
2 Heat a grill pan or barbecue then grill the fish with the slices of kamokamo for 3 minutes each side before turning over, season then repeat.
3 Serve the fish with the kamokamo, a spoonful of pesto and a wedge of lemon.


text:
Robert Redford stars as a man trying to survive solo while adrift at sea in the sophomore feature from Margin Call helmer JC Chandor. After premiering to strong reviews out of Cannes, All Is Lost is conspicuously skipping September's Toronto Film Festival and could make its North American debut at Telluride ahead of awards season. Lionsgate and Roadside Attractions release the pic October 18. Here's the trailer:
Hot Trailer: Robert Redford's 'All Is Lost'
9 Comments
Metlife of Pi
I'm in!
Jeremiah Johnson 2: Open Water
(but srsly, looks great)
I really like Margin Call and will give this a spin.
awful trailer… I've pretty much seen most of the film now… minus the obligatory rescue hysterics
Yes, please…
If this is as good as everyone says, it's really going to steal the thunder of Angelina Jolie's adaptation of UNBROKEN. The first half of the book (and just as powerful as the POW sequences in the second half) is all about the protagonist surviving weeks alone out to sea.
Grateful these films are still being made, and for a price. Smart business of worthy content.
And the Oscar goes to…


text:
Busting Loose (1977– )
Plot Summary
Following his graduation from engineering school, 24-year-old slacker Lenny Markowitz moves into his own rundown apartment (his first time away from home), and next door to a gorgeous older redhead (Melody). Ignoring his engineering background, he goes to work at a shoe store owned by Cabell and staffed by jive-talking Raymond. He also finds time to play poker and get into trouble with his childhood pals.- Written by Marty McKee <firstname.lastname@example.com>
Single guy Lenny deals with his meddling parents, boisterous pals and menial job.- Written by Marty McKee <email@example.com>
Be the first to contribute! Just click the "Edit page" button at the bottom of the page or learn more in the Synopsis submission guide.


text:
Energy-oriented design tools for collaboration in the cloud
Toth, Bianca, Salim, Flora, Burry, Jane, Frazer, John H., Drogemuller, Robin, & Burry, Mark (2011) Energy-oriented design tools for collaboration in the cloud. International Journal of Architectural Computing, 9(4), pp. 339-360.
Accepted Version
(PDF 901kB)
View at publisher (open access)
Emerging from the challenge to reduce energy consumption in buildings is the need for energy simulation to be used more effectively to support integrated decision making in early design. As a critical response to a Green Star case study, we present DEEPA, a parametric modeling framework that enables architects and engineers to work at the same semantic level to generate shared models for energy simulation. A cloud-based toolkit provides web and data services for parametric design software that automate the process of simulating and tracking design alternatives, by linking building geometry more directly to analysis inputs. Data, semantics, models and simulation results can be shared on the fly. This allows the complex relationships between architecture, building services and energy consumption to be explored in an integrated manner, and decisions to be made collaboratively.
Impact and interest:
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These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.
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text: Does anyone else have problems with their front driver and passenger door windows making noise? It seems like when the weather is cold, doesn't get very cold in So. Cal, my windows sqeaks when they are being rolled up and down. I brought this to the dealers attention, all they did was clean the window seals and told me it was dirt buildup. I am so tired of the noise, can someone help me out? As every year pass, the noise seems to get worse.


text:
Oregon food stamp recipients thankful for help
More Americans using them for this year's Thanksgiving
BEND, Ore. - The number of people depending on food stamps for Thanksgiving dinner is not surprising to Gene Evans, communications director at the Oregon Department of Human Services.
While many of us have trouble making room on our plates, others are struggling to provide just a basic meal.
The Economic Policy Institute says 42 million Americans will be on food stamps this Thanksgiving. That's about the size of the population of California and Connecticut combined.
And the numbers here in Oregon and in Central Oregon are high as well.
Oregon's food stamp rolls have been increasing since the recession began in 2008.
"More and more families are having to depend on state assistance and food stamps in order to feed their families," Evans said Wednesday.
The state of Oregon has added about 300,000 people to SNAP, Supplemental Nutrition Assistance Program, formerly known as food stamps.
More than 800,000 people in Oregon and more than 46,000 people in our three counties are receiving the benefits.
And for a time when people really focus on food, Thanksgiving is especially difficult for those trying to make ends meet.
"When a family is fighting through and working through hunger and unemployment and those kind of issues puts a focus on something that is difficult to deal with," said Evans. "(Especially) on a holiday that is really about turkey and desserts and all the things that all of us take for granted and are thankful for."
Evans said food stamp benefits are a direct result of the states employment situation -- people who are out of work, work part-time or their work hours have been cut back.
It's not a lot of money; an average family receives just $250 per month.
"But it's enough money to keep a family safe, keep a family stable and keep the children fed, or seniors or grandparents fed," Evans said.
Evans pointed out that the department receives letters from children and single parents who say they wouldn't be able to make ends meet without the benefit every month.
"It truly is one of the safety net programs," Evans said. "So yes, people have expressed their thankfulness for that."
Evans told NewsChannel 21 that the use of food stamps will go down as more and more people go back to work.
He explained that it takes a little bit of time for people's incomes to get back into normal routine before they can drop their eligibility for food stamps.
He expects the numbers to continue to stay high into 2013, emphasizing that it's going to take a long time to get back to the pre-recession level.


text:
The Book Of Cain/The First Dynasty
Book by: Consul Moore
Status: Finished
Genre: Historical Fiction
Chapter1 (v.1) - Introduction: How This Ancient Book Was Discovered
An American Soldier was on an important mission on May 1st 2011, his Unit was sent into the depths of a cave in the mountains of Afghanistan. This soldier, whose name cannot be given, was separated from his Unit during a firefight. Only rumors are spoken of the event where the fighting commenced and there is no evidence. The firefight is said to have developed when the soldiers involved came across an unusually large man found within a spacious cavern. The soldier who was separated from his Unit never saw the phenomenon himself, for as soon as shots were fired from those in front of him he was knocked back, and then he stumbled into a hole. He recalled sliding down a tunnel, and he said everything was dark as he went down, then suddenly he was in the air for a moment before he landed safely on top of a mound of sand. He proceeded to roll down the sand hill and when he came to a stop he quickly reached for his flashlight. When the light shined he was in shock over what he saw, for the walls of the cavern looked to be covered in bronze. The soldier said he wandered around the cavern for a while, trying to get a better look at the writing on the walls, and looking for some way out. As he headed further down the sand dune he noticed a smaller object which shined whenever he flashed his light in its direction. As he drew nearer to this object he saw what looked like large black marble hands emerging from the sand and holding up a smaller bronze object, a book unlike any he had seen before.
The book this heroic soldier discovered is about the size of an Old Catholic Bible, (the font used was quite large but the entire text is not as large as the Torah). The stories inside this text are different from the ones found in the Dead Sea Scrolls. Although there are some familiar names which have been mentioned in the Torah, and other ancient texts.
The soldier was found by his comrades, who sent down a rope into the cavern so as to retrieve him. He placed the book into his assault pack before grabbing the rope sent down for him. He kept this book hidden from the other soldiers for the rest of his deployment. He said that in knowing he could not bring the antiquity onto the plane with him when it came time for him to redeploy home; he decided to send the book back in his Unit's conex container along with some of his other goods. He did this a month before he was scheduled to come home. He was able to do this because he knew the soldiers who were inspecting the packed items. At this time the inspections were random. His unit was small and the process was simple. His friends gave him a pass after he told them he was smuggling some Cuban cigars. As soon as the conex container was packed and closed upon his watch, he knew that no one would open the container till he was returned home with his Unit.
If not for this soldier, I would have never been able to share these ancient narratives with you today. He took a risk for what he believed to be a treasure that needed to be safely handled. For a reason which I'm not at liberty to elaborate upon, this soldier lost trust in the organization in which he served. The day the conex container was reopened in the states the soldier was waiting there to retrieve his bag. As soon as he picked up his items, and saw the book was where he left it, he went straight into his vehicle and headed towards Claremont California, to see me, his Uncle.
I will not bore you with the details which were involved in me determining the age of this antediluvian bronze book, but I will say that there were at least four different authors who contributed to the writing. And because there are different authors involved some of the chapters are written in the third person, and some are written in first person. Some of the story is very much like a modern day novel, where we can feel and visualize the protagonist, while being able to relate with their personal struggle. Still other chapters simply state what occurred, being written in a style that is similar to the writings of the Old Testament. After reading the entire Sumerian and Hebrew like text, it was interesting to discover that as a whole this may be the most descriptive and imaginative of all ancient writings, even Homer's. So, I've decided to just share the stories with you in the hope that you'll read the text in the same manner as you would an exciting and romantic novel.
Now before Smithsonian came to "acquire" the bronze book, I had already taken photographs of each of the pages within. After a year of translating the text I felt deeply compelled to share the story I had discovered. Even though I was warned not to share this content with the public, I have no fear in doing so now, because after all I've published this novel under the genre of fiction.
Thank you for enduring my brief Introduction, and I hope you enjoy the first part in this amazing tale as much as I enjoyed translating it and placing the text in an order I feel raises the entertainment value.
The discovery of the original text was not titled, but because of the first sentence I have decided upon the following title; The First Dynasty-The Rise of Cain. Perhaps simply The Book of Cain would be better.
*1. The bronze book is made of copper alloy, more copper than tin. The Hebrew and Sumerian text within the book has been burnt into the bronze, by what tool we cannot be certain, but we expect the writing instrument was heated steel. Ultimately how the text was written into the bronze may remain a mystery.
During the reading of this story whenever you see a *, know that further explanation on the passage you've just read can be referenced at the end of the chapter.
© Copyright 2017 Consul Moore. All rights reserved.
Add Your Comments:
Other Content by Consul Moore
Short Story / Mystery and Crime
Short Story / Young Adult
Book / Humor


text:
Club Headlines
Biosteel Joins as Sports Drink of RSB : BRAMPTON, ON. (Friday, September 23, 2016) – Biosteel has become the Official Sports Drink Partner of Red Star Brampton.
Red Star Secure Division Title : MISSISSAUGA, ON - Red Star Brampton Soccer Club has finished the 2016 indoor season as the Hershey D5 South Conference champions and...
Tatra Matchday Partner of Red Star : BRAMPTON, ON. (Tuesday, May 12, 2015) – Tatra Beer has become the Official Matchday Partner of Red Star Brampton.
2015 Season Opener in Norton Park : BRAMPTON, ON. (Tuesday, April 28, 2015) – Red Star Brampton will play out the upcoming 2015 season in the BASL (Brampton Adult Soccer ...
2015 Season Free Agent Camps : BRAMPTON, ON. (Friday, April 24, 2015) – Red Star Brampton will be offering specific recruitment training sessions for the 2015 Presea...
SN Truck Services become Red Star Sponsor : BRAMPTON, ON. (Monday, January 26, 2015) – Stan Niemczyk Truck Service Inc. (SN Truck Service Inc.) have become an official sponsor of ...
Red Star: 2014 PCSL Semi-Final Closing : BRAMPTON, ON - Red Star Brampton Soccer Club ended the season with a Fourth Place regular season and Semi-Final Playoff finish in the...
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AAMT Book of Style, Second Edition, Electronic provides medical language professionals with hands-on access to the content in the Book of Style manual.Welcome to the Australian Association of Massage Therapists Your application for membership has been processed and you will have been mailed a letterMaybe some day there will be other associations to choose from for us to belong to, but right now all we have is AAMT and I think it should be supported.Basic Style Guide Courtesy of Global Medical Transcription and MT Daily The 2005 changes made by AAMT are not included in this style guide. These are the generally ...2/8/2012 · Long known as 'the gold standard' for medical transcriptionists, The AAMT Book of Style for Medical Transcription addresses points of grammar, punctuation ...Join us for The Book of Style Boot Camp Series. This series was devised to introduce both experienced healthcare documentation specialists and those new to the ...Medical Transcription Guide: Do's and Don'ts, 3e [Marcy O. Diehl BVE CMA-A CMT AHDI-F] on Amazon.com. *FREE* shipping on qualifying offers. A quick reference for …Serving Over 20,000 US Medical Transcriptionists: Guide wire or guidewire? Posted By: CB on 2008-08-23 In Reply to: Subject: Guide wire or guidewire?Serving Over 20,000 US Medical Transcriptionists: Glidewire vs guide wire Posted By: stumped on 2006-04-27 In Reply to: The cardiac guys/gals love new stuff!Medical Clip Art. Medword Medical Transcription sells TENS machines, transcriber headsets, WAVpedal foot pedals. Digital voice recorders, TENS units and dictation ...
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German Shepherd Dog Forums (http://www.germanshepherds.com/forum/)
- Pictures! Pictures! Pictures! (http://www.germanshepherds.com/forum/pictures-pictures-pictures/)
- - Coda, 15 weeks (http://www.germanshepherds.com/forum/pictures-pictures-pictures/399129-coda-15-weeks.html)
Coda, 15 weeks
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Hi, I haven't posted in awhile so I thought I'd share some new pictures of Coda. I'm still not sure of her mix, my best guess stands at German Shepherd/Dutch Shepherd/???. The spots on her paws totally throw me off. She's growing up so beautifully, and is a whopping 23lb. today. Here's some pics from the past month.
very cute, looks like she has lots of personality
Very cute but I see pit bull not dutchie.
Adorable!! :)
She is a beautiful girl. You can tell from her facial expression that she knows that she is special.
Hey! I thought I recognized you from another forum and I was correct... welcome to the dark side! :)
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10,122 of 127,809 schools have worse air.
Note: Rankings are based on modeled concentrations and severity of chemicals known or believed to cause cancer. Learn More
Note: Rankings are based on modeled concentrations of chemicals that cause health problems other than cancer. Learn More
Definition: Manganese is used to produce pesticides, batteries and other industrial products. Although trace elements are found in food and are considered essential for good health, overexposure for long periods can cause mental and emotional disturbances and slow and clumsy body movements -- a disease of the brain called manganism. Children are more vulnerable to such exposures than adults. EPA says that it cannot be determined if there is a link between manganese and cancer.
Definition: Nickel, a metal, can be combined with other metals to make coins, jewelry, and stainless steel. People working in nickel refineries or nickel-processing plants have experienced chronic bronchitis, reduced lung function, and lung and sinus cancers. EPA classifies nickel refinery dust and nickel subsulfide as human carcinogens. Source: EPA, HHS
Definition: Diisocyanates are a group compounds used to manufacture foams, fibers, insulation, paints and varnishes, particularly in the automobile industry. Workers exposed to diisocyanates may develop serious or fatal respiratory diseases, including asthma, says the National Institute for Occupational Safety and Health. The World Health Organization has recommended that diisocyanates be treated as a potential human carcinogen.
Definition: Epichlorohydrin, a colorless liquid, is used to produce glycerin, plastics, paints and insect fumigants. Based on animal studies, epichlorohydrin is reasonably anticipated to be a human carcinogen, says the International Agency for Research on Cancer. One study of factory workers found an elevated level of respiratory cancers, though they were also exposed to other chemicals.
Definition: Arsenic occurs naturally in the earth's crust. When combined with oxygen, chlorine and sulfur, inorganic arsenic is used to preserve wood. Organic arsenic compounds are used as pesticides. EPA and the U.S. Department of Health and Human Services have concluded inorganic arsenic compounds are known carcinogens, linked to skin cancer as well as cancers of the liver, bladder and lungs.
Sources: U.S. Environmental Protection Agency, University of Massachusetts at Amherst Political Economy Research Institute
* Chemicals most responsible for the toxicity outside this school may not add up to 100% because only the top chemicals are listed.


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The full trailer for the "Nazis from the moon" invaders flick is now online . . .
It feels like a gazillion years ago since we announced the making of Iron Sky – about Nazis from the moon invading Earth.
In the meantime the Finnish film-makers (who also did Star Wreck, which took seven years to complete) not only managed to get funding for their project, but completed the movie.
Iron Sky doesn't have any distributors in the States yet, but the movie is going to be shown at a film festival soon.
To be honest the visual FX look excellent for such a low-budget affair (it was made for €7,500,000) and we think it's going to be lots of fun.
Anyway, here're the trailer and some pics:
About the Author
James has been running The Sci-Fi Movie Page since Before the Beginning of Time Itself (TM), i.e. since the site's inception in 1997. In addition to sci-fi James also likes 1970s motorbikes and chili dogs although he doesn't own the former and no longer eats the latter. He currently resides in Kiev, Ukraine for reasons best left unexplained.


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Morphine has been reported to accelerate the progression of chronic kidney disease. However, whether morphine affects slit diaphragm (SD), the major constituent of glomerular filtration barrier, is still unclear. In the present study, we examined the effect of morphine on glomerular filtration barrier in general and podocyte integrity in particular. Mice were administered either normal saline or morphine for 72 h, then urine samples were collected and kidneys were subsequently isolated for immunohistochemical studies and Western blot. For in vitro studies, human podocytes were treated with morphine and then probed for the molecular markers of slit diaphragm. Morphine-receiving mice displayed a significant increase in albuminuria and showed effacement of podocyte foot processes. In both in vivo and in vitro studies, the expression of synaptopodin, a molecular marker for podocyte integrity, and the slit diaphragm constituting molecules (SDCM), such as nephrin, podocin, and CD2-associated protein (CD2AP), were decreased in morphine-treated podocytes. In vitro studies indicated that morphine modulated podocyte expression of SDCM through opiate mu (MOR) and kappa (KOR) receptors. Since morphine also enhanced podocyte oxidative stress, the latter seems to contribute to decreased SDCM expression. In addition, AKT, p38, and JNK pathways were involved in morphine-induced down regulation of SDCM in human podocytes. These findings demonstrate that morphine has the potential to alter the glomerular filtration barrier by compromising the integrity of podocytes.
Citation: Lan X, Rai P, Chandel N, Cheng K, Lederman R, Saleem MA, et al. (2013) Morphine Induces Albuminuria by Compromising Podocyte Integrity. PLoS ONE 8(3): e55748. https://doi.org/10.1371/journal.pone.0055748
Editor: Maria Pia Rastaldi, Fondazione IRCCS Ospedale Maggiore Policlinico & Fondazione D'Amico per la Ricerca sulle Malattie Renali, Italy
Received: July 10, 2012; Accepted: December 31, 2012; Published: March 29, 2013
Copyright: © 2013 Lan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants RO1DK084910, RO1DK083931 (PCS) and HL68802, HL103773 (KG) from National Institutes of Health, Bethesda, MD. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Morphine, a metabolite of heroin, is the main stay of pain management after surgery, angina, myocardial infarction, and trauma [1]. However, heroin has also been one of the main drugs abused for addiction, and has significantly contributed to morbidity and mortality since the early 1990s in the United States [2], [3], [4], [5].
Morphine has been reported to exert a bimodal effect on the growth of kidney fibroblasts and glomerular mesangial cells [6], [7], [8], [9], [10]. Intravenous opiate addiction has also been considered a risk factor for the development of human immunodeficiency (HIV)-associated nephropathy [11], [12]. Johnson et al reported that morphine administration altered the microprojections on podocytes [13]. We also reported earlier that morphine promoted glomerular epithelial cell proliferation at lower concentration while triggered apoptosis at higher concentration [8]. We demonstrated that morphine-induced alteration in epithelial cell phenoytype was mediated by alteration of hemoxygenase (HO)-1 activity; however, in these studies, we did not study any loss of visceral epithelial cell (podocyte) markers in response to morphine [8]. Thus, the present study not only provides data on the effect of morphine on glomerular visceral epithelial cells (podocytes) in vivo, but also examines the effect of morphine on the markers of visceral epithelial cell integrity.
As the major constituent of glomerular filtration barrier (GFB), slit diaphragm (SD) plays an important role in the prevention of glomerular protein leakage both in physiological and in pathological states. SD consists of proteins specifically expressed by podocytes, such as nephrin, podocin, CD2-associated protein (CD2AP), and synaptopodin [14]. On that account, the podocyte is considered the key cell contributing to the development of albuminuric kidney diseases. Decrease of nephrin and podocin has been shown to be linked with albuminuria and progressive renal disease [15], [16]. Whether morphine impairs the SD constituting molecules (SDCM) has yet to be studied.
In the present study, we evaluated the effect of morphine on glomerular filtration barrier in general and podocyte integrity in particular. We showed that morphine-receiving mice not only developed albuminuria but also displayed attenuated expression of SDCM in human podocytes. We also demonstrated that morphine-induced loss of podocyte integrity through loss of slit diaphragm associated molecules is mediated by reactive oxygen species (ROS) generation. These findings provide a basis to support the hypothesis that morphine-induced direct impairment of podocytes contributes to kidney disease.
Materials and Methods
Morphine pellets were obtained from National Institute on Drug Abuse (Bethesda, MD, USA). For in vitro study, morphine was dissolved in normal saline, stored at a concentration of 10−1 M, and used in concentrations of 10−10 to 10−6 M. [D-Pen2,5]-Enkephalin (DPDPE), [D-Ala2, N-Me-Phe4, Gly5-ol]-Enkephalin (DAMGO), 2-(3,4-Dichlorophenyl)-N-methyl-N-[(1S)-1-(2-isopropyl)-2-(1-(3-pyrrolinyl))ethyl] acetamide hydrochloride (LPK26) were obtained from Sigma Aldrich (St. Louis, MO). LY294002, SP600125, and SB203580 were purchased from Cell Signaling Technology (Danvers, MA).
Animal experiments
All animal experiments complied with Institutional Animal Care and Use Committee (IACUC)-approved protocols. FVB/N mice (purchased from Jackson Laboratory, eight in each group) were administered either normal saline or morphine (by subcutaneous implantation of a 75-mg slow-release morphine pellet). After 72 h, spot urine samples were collected by giving gentle pressure over the urinary bladder. The mice were subsequently sacrificed and the kidneys were isolated for renal immunohistochemical and Western blotting studies. Albumin and creatinine in the urine were measured with Albuwell M Test Kit (Cat# 1011, Exocell, Philadelphia, PA) and Creatinine Companion (Cat# 1012, Exocell), respectively, following the instruction of the manufacturer.
Culture of human podocytes
Human podocytes were cultured as previously reported [17]. Briefly, immortalized human podocytes proliferated in the growth medium containing RPMI 1640 supplemented with 10% fetal bovine serum, 1 X pecicillin-streptomycin, 1 mM L-glutamine, and 1 X insulin, transferrin, and selenium (ITS) (Invitrogen, Grand Island, NY) at permissive temperature (33°C). When the cells reached about 80% confluence, they were transferred to 37°C for differentiation in a medium without ITS for 7 days.
Total RNA was isolated from human podocytes using Trizol reagent (Invitrogen). Five micrograms of total RNA were reverse transcribed using the first-strand synthesis system (Invitrogen). PCR was performed by using Platinum PCR SuperMix High Fidelity (Invitrogen). Sequences of primers for human opiate receptor genes were: ACGTGCTTGTCATGTTCGGCATCGT (DOR-FW), ATGGTGAGCGTGAAGATGCTGGTGA (DOR-RV), GATACACAAAGATGAAGACAGCAACCAAC (KOR-FW), TCCCTGACTTTGGTGCCTCCAAGGACTATT (KOR-RV), GATCATGGCCCTCTACTCCA (MOR-FW), and GCATTTCGGGGAGTACGGAA (MOR-RV). GAPDH was used as internal control, and the primers were GGGAAGCTCACTGGCATGGCCTTCC (GAPDH-FW) and CATGTGGGCCATGAGGTCCACCAC (GAPDH-RV). Amplification was performed at 95°C for 5 min, followed by 30 cycles at 94°C for 1 min, 55°C for 30 s, 68°C for 1 min with a final extension cycle for 5 min at 68°C. DNA samples were visualized by 2% agarose gel electrophoresis.
Immunofluorescent microscopy
Immunofluorescent microscopy was performed as previously reported [18]. Briefly, the kidneys were perfused in situ and then fixed with fresh 4% PFA and stored at −80°C. Subsequently, paraffin sections (4 μm) were prepared and de-paraffinized in xylene and re-hydrated through graded concentrations of alcohol. Epitope retrieval was carried out by heating the samples at 98°C for 2 h in Retrieveall-1 (Signet Laboratories, Inc.). Subsequently, cooled samples were permeabilized with 0.3% triton X-100 for 10 min, and were blocked with 2% BSA in 0.1% triton X-100 for 1 h at room temperature. Sections were then incubated with primary antibodies overnight at 4°C, followed by Alexa Fluor secondary antibodies (Invitrogen, 1∶800), donkey anti-rabbit IgG Alexa Fluor 488, donkey anti-goat lgG Alexa Fluor 594, goat anti-mouse IgG Alexa Fluo 488, or goat anti-rabbit IgG Alexa Fluo 568, for 1 hour at room temperature. Primary antibodies included rabbit anti-Mu/Kappa/Delta opiate receptors (Abcam, 1∶200), rabbit anti-synaptopodin (Santa Cruz, 1∶100), mouse anti-8-OHdG (Santa Cruz, 1∶100), and goat anti-nephrin (R&D systems, 1∶50). All antibodies were diluted in 0.1% Triton X-100, 2% BSA in PBS. Cells were then counterstained with DAPI to identify nuclei (Sigma-Aldrich). Morphological changes were visualized and captured with a ZEISS microscope (Carl Zeiss MicoImaging GmbH, Jena, Germany) equipped with a digital imaging system.
Western blot analysis
Cells were rinsed twice with PBS and lysed by M-PER Protein Extraction Buffer (Pierce, Rockford, IL) containing 1× protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN). Proteins (20–30 μg) were separated by 10% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to an Immuno-Blot polyvinylidene fluoride (PVDF) membrane (Bio-Rad, Hercules, CA). After blocking in PBS/Tween (0.1%) with 5% nonfat milk, the membrane was incubated with primary antibodies overnight at 4°C followed by horseradish peroxidase-conjugated secondary antibodies (Santa Cruz, 1∶3000) and then developed using Enhanced Chemiluminescent (ECL) solution (Pierce). Primary antibodies included rabbit anti-nephrin (Santa Cruz, 1∶1000), goat anti-synaptopodin (Santa Cruz, 1∶1000), mouse anti-CD2AP (Santa Cruz, 1∶1000), rabbit anti-podocin (Sigma, 1∶200), goat anti-actin (Santa Cruz, 1∶1000), rabbit anti-phospho-JNK (Cell Signaling, 1∶1000), rabbit anti-JNK (Cell Signaling, 1∶1000), rabbit anti-phospho-Akt (Cell Signaling, 1∶1000), rabbit anti-Akt (Cell Signaling, 1∶1000), rabbit anti-phospho-p38 (Cell Signaling, 1∶1000), and rabbit anti-p38 (Cell Signaling, 1∶1000). For data quantification, the films were scanned with a CanonScan 9950F scanner and the acquired images were then analyzed using the public domain NIH image program (developed at the U.S. National Institutes of Health and available on the internet at http://rsb.info.nih.gov/nih-image/).
Intracellular ROS measurement
Human podocytes were differentiated in 96-well plates for 7 days as mentioned above, and were then cultured in serum free medium for 12 h. Subsequently, 10−10 to 10−6 M morphine was added. After incubation for another 12 h, intracellular ROS was determined by analyzing the fluorescence intensity of the intracellular fluoroprobe 2, 7-dichlorofluorescin (DCFH) (Molecular probe, Carlsbad, CA, USA), following the manufacturer's instructions.
Electron Microscopic (EM) studies
Mice in groups of five were administered either normal saline or morphine. Kidneys were harvested and fixed in glutaraldehyde and embedded in EPON. Thin sections were cut and reviewed and examined under a transmission microscope. All the EM sections were reviewed in a blinded fashion. Pictures from each kidney were acquired in a double blind manner using sample numbers without the identity of the mouse.
Statistical analyses
Data were presented as means ± standard deviation (SD) unless otherwise noted. All experiments were repeated at least three times with duplicate or triplicate samples in each assay. All data were evaluated statistically by the analysis of variance (ANOVA), followed by Nweman-Keuls multiple comparison tests using software (Prism 4.0, GraphPad Software). In the case of single mean comparison, data were analyzed by t test. P values <0.05 were regarded as statistically significant.
Classic opiate receptors are present on podocytes
Opiate receptors (ORs) have been reported to express in kidney messangial cells [6], but their expression in podocytes has not been studied. Therefore, we examined the expression of ORs in podocytes. In in vitro study, we conducted RT-PCR analysis by using human podocytes as the RNA source. The results revealed high level expression of KOR and MOR in human podocytes, but the expression of DOR was barely detectable (Fig. 1). To confirm this observation in in vivo studies, we performed immunofluorescence staining of mouse kidney sections. Both KOR and MOR were highly expressed in kidney, and they were colocalized with podocyte marker nephrin; while no DOR expression was found in podocyte (Fig. 2). These results demonstrate that podocytes display expression of KOR and MOR only.
Total RNAs were prepared from human podocytes (labeled as P1, P2), and were used for RT-PCR to detect the expression of delta opiate receptor (DOR), kappa opiate receptor (KOR), and mu opiate receptor (MOR). RNAs from human fetal brain (B) were used as positive control. GAPDH was used as internal control.
Paraffin sections were prepared from 2-month-old mice kidneys, and immunofluorescence staining was performed to detect the expression of opiate receptors. Nephrin was used as a marker of podocytes.
Morphine treatment leads to acute albuminuria
It has been reported that chronic morphine treatment leads to glomerulophathy particularly proteinuria [5]. To examine the effect of morphine on podocytes, we treated the mice with high dose morphine as described in Materials and Methods. Three days after the treatment, mouse urine samples were collected, and total albumin and albumin/creatinine ratio were determined. The histology in Periodic acid-Schiff (PAS)-stained mouse kidney sections was also examined. Although we didn't find obvious differences between the control and treatment groups in the histology (data not shown), we did observe that high dose morphine treatment significantly increased both total albumin and albumin/creatinine ratio in the urine (Fig. 3). These results suggest that morphine treatment directly impairs the blood urine barrier.
Two-month old mice were administered with either normal saline or morphine by subcutaneous implantation of a 75-mg slow-release morphine pellet. After 72 h urine was collected, and the amount of albumin (A) and albumin/creatinine ratio (B) were determined.
Morphine treatment compromises podocyte integrity
To determine the effect of morphine on podocytes, we performed immunofluorescent staining of mouse kidney sections and immunoblotting studies of the kidney lysates. Morphine attenuated the expression of podocyte specific molecules such as synaptopodin and nephrin (Fig. 4, A–D). In vitro study also showed that morphine treatments decreased the protein expression of SDCMs in human podocytes (Fig. 5). Transmission electron microscopy of the mouse kidney sections showed that morphine administration increased the podocyte foot process effacement (Figure 4E). These results indicate that morphine treatment may compromise podocyte integrity.
A. Two-month-old mice were administrated either normal saline or morphine for 3 days. Frozen sections were prepared for immuno-fluorescence staining. B. kidneys were homogenized, and the tissue lysates were subjected to Western blot to detect the changes of nephrin and synaptopodin. C–D. Quantification of the expression of nephrin (C) and synaptopodin (D), and the results (mean ± SD) represent three independent samples. E. A representative microphotograph from transmission electron microscopy studies. Foot processes are indicated by arrows. Foot processes are well delineated and are not fused in the control, while they are fused (effaced) in morphine-receiving mice. The scale bar is 500 nm. Mag. 270,000X.
A. Differentiated human podocytes were treated with 10−8 or 10−6 M morphine for 24 h, and the cell lysates were collected for Western blots. B–E. Quantification of the expression of nephrin (B), synaptopodin (C), CD2AP (D), and podocin (E) in A, and the results (mean ± SD) represent three independent samples. * p<0.05 compared with control (0 M).
Morphine mediates alterations in podocyte SDCMs via MOR and KOR
Weber et al reported that morphine affects mesangial cells mainly via KOR but not MOR [6]. To determine the receptors involved in podocytes, we stimulated human podocytes with OR-specific agonists. Western blot results revealed that both KOR-specific agonist LKD26 and MOR-specific agonist DAMGO decreased the expression of SDCMs, while DOR-specific agonist DPDPE did not show this function (Fig. 6). These results indicate that morphine-induced alterations in podocyte SDCM occurred via both KOR and MOR.
A. Human podocytes were treated with agonists specifically for DOR (labeled as D), KOR (K), or MOR (M) for 24 h, and the cell lysates were collected for Western blots. B–E. Quantification of the expression of nephrin (B), synaptopodin (C), CD2AP (D), and podocin (E) in A, and the results (mean ± SD) represent three independent samples. * p<0.05 compared with control (CN).
Morphine induces podocyte DNA damage through ROS generation
Our group and others have demonstrated that ROS is a significant contributing factor for podocyte injury and for the progression of chronic kidney disease [8], [19], [20]. ROS has also been shown to damage SD [21], [22], [23]. To examine the effect of morphine on intracellular ROS production, the fluorescence intensity of the intracellular fluoroprobe (DCFH) was evaluated. Results showed that ROS generation was significantly increased in morphine-treated cells as compared with unstimulated cells (Figure 7). Morphine increased ROS generation in a dose-dependent manner (Figure 7A).
A. Human podocytes were treated with 10−10 to 10−6 M morphine for 12 h, and were labeled with DCFH for 30 min. After washing with PBS, the cells were incubated at room temperature for 2 h, and the ROS generation was determined. * p<0.001 compared with control (0 M). B. Kinetics of ROS generation for 10−6 M morphine treatment. C. Paraffin sections were prepared from control or morphine-receiving mice, and immunofluorescent staining was performed to detect the nuclear expression of 8-OHdG, a molecular marker of oxidative damage to DNA. WT1 was used as a marker of podocytes.
To determine the effect of morphine-induced ROS generation on oxidative DNA damage in the podocyte, we carried out immunofluorescent staining of podocytes for 8-hydroxyguanine (8-OHdG), a molecular marker of oxidative damage to DNA, in morphine-receiving mice. Morphine-receiving mice displayed increased positive staining of 8-OHdG in podocytes (Figure 7C).
To test the effect of oxidative stress on SDCMs, aliquots of variable concentrations of hydrogen peroxide (H2O2) were added to the media of human podocytes for 24 h. Western blotting studies revealed that H2O2 decreased SDCMs expression in a dose-dependent manner (Figure 8). To further confirm these observations, podocytes were pre-treated with either superoxide dismutase or catalase and then incubated in media containing H2O2. As shown in Figure 9, pretreating the human podocytes with SOD or catalase significantly attenuated morphine-induced compromise of SDCMs. Taken together, these data suggest that morphine-induced ROS generation may be a contributor to the decrease of SDCMs.
A. Human podocytes were treated with 1, 10, or 50 µM H2O2 for 24 h, and the cell lysates were collected for Western blots. B–E. Quantification of the expression of nephrin (B), synaptopodin (C), CD2AP (D), and podocin (E) in A, and the results (mean ± SD) represent three independent samples. * p<0.05, ** p<0.01 compared with control (0 µM).
A. Human podocytes were pretreated with SOD or catalase for 1 h before 10−6 M morphine was added. After another 24 h incubation at 37°C, the cell lysates were collected for Western blot. B–E. Quantification of the expression of nephrin (B), synaptopodin (C), CD2AP (D), and podocin (E) in A, and the results (mean ± SD) represent three independent samples. * p<0.05 compared with blank control, while # p<0.05 compared with morphine treatment alone.
AKT, JNK, and p38 pathways are involved in opioid-induced podocyte injury
Several kinases, including AKT, JNK, Erk1/2 and p38 have been implicated in podocyte injury and the progression of chronic kidney diseases (CKD) [6], [15], [24], [25], [26]. A transcription factor, signal transducer and activator of transcription 3 (STAT3), has also been reported to regulate HIV-induced podocyte proliferation [24]. To examine the involvement of these kinases and factors in morphine-induced down-regulation of SDCMs, we first evaluated the phosphorylation of these proteins. We treated the human podocytes with morphine, and collected the cell lysates at different time points for Western blot studies. Results showed that morphine stimulation significantly activated AKT, JNK, and p38 at early time points (Fig. 10), while neither Erk1/2 nor STAT3 could be activated by morphine at any time point (data not shown). We also used KOR- and MOR-specific agonists, LKD26 and DAMGO, to stimulate the human podocytes, and the same activation results with morphine were observed (data not shown). These results suggest that morphine down-regulates SDCMs via AKT, JNK, and p38, but not Erk1/2 or STAT3.
Human podocytes were starved in serum free medium for 12 h, and then 10−6 M morphine was added. Cell lysates were collected at different time points for Western blot.
To further examine the role of activation of AKT, JNK, and p38 in morphine-induced down-regulation of SDCMs, the expression of SDCMs was measured in podocytes treated with morphine in the presence or absence of LY294002, an inhibitor of PI13/AKT, SP600125, an inhibitor of JNK, or SB203580, an inhibitor of p38. As presented in Figure 11, all these inhibitors partially attenuated morphine-induced down-regulation of SDCMs. These results indicate that AKT, JNK, and p38 pathways are involved in the regulation of morphine-induced impairment of SDCMs.
A. Human podocytes were pretreated with of LY294002 (LY), SB203580 (SB), or SP600125 (SP) for 1 h before 10−6 M morphine was added. After another 24 h incubation at 37°C, the cell lysate were collected for Western blot. B–E. Quantification of the expression of nephrin (B), synaptopodin (C), CD2AP (D), and podocin (E) in A, and the results (mean ± SD) represent three independent samples. * p<0.05 compared with blank control, while # p<0.05 compared with morphine treatment alone.
Morphine has been reported to cause kidney cell injury in both in vitro and in vivo studies [7], [8], [9], [10], [11], [12]. In the current study, we observed that administration of morphine contributed to albuminuria which appeared to be glomerular in origin and a consequence of the loss of slit diaphragm integrity [27]. Both in vivo and in vitro data confirmed that morphine inflicted podocyte injury in the form of attenuated expression of SDCMs (Figure 4 and 5); these findings further confirmed that loss of integrity of SD contributed to albuminuria in morphine-receiving mice. Since these SDCMs are actin-associated molecules, the reduced level of expression of these molecules by morphine may change the actin cytoskeleton organization. Transmission electron microscopy of the mouse kidney sections showed effacement of podocyte foot process in morphine-receiving mice (Figure 4E), which might be due to the change of the actin cytoskeleton organization. However, further studies are needed to explore this aspect.
In previously reported studies, morphine has been demonstrated to exert bimodal effects (both apoptosis and proliferation) on glomerular epithelial cells [8]; however the role of opiate receptors was not evaluated in these studies. In the current study, we found that KOR and MOR, but not DOR, were expressed in both human and mouse podocytes (Figures 1 and 2). We also found that stimulating either KOR or MOR in podocytes could activate related kinases and down-regulate SDCMs. To our knowledge, this is the first report on the opiate receptors in podocytes. Weber et al reported that although both KOR and MOR were expressed in glomerular mesangial cells, only KOR played the function to activate STAT3 and led to the proliferation and glomerulopathy [6]. These findings indicate that podocytes behave differently from mesangial cells in morphine milieu.
Oxidative stress is a common cause of cellular injury. Several investigators have demonstrated that enhanced ROS generation leads to albuminuria by damaging SD components [21], [28], [29]. Morphine has been demonstrated to stimulate the production of superoxide by macrophage and mesangial cells [22], [23]. As we previously reported, generation of ROS induces apoptosis of rat glomerular epithelial cells [8]. Here we also found that morphine increased ROS generation in human podocytes in a dose-dependent manner (Figure 7A). Addition of H2O2 to the media decreased the expression of SDCMs (Figure 8), while free radical scavengers prevented this damaging effect of morphine (Figure 9). All these findings strongly suggest the role of ROS in morphine-induced down-regulation of SDCMs. One potential possibility is that ROS induced injury hampers the expression of SDCMs. Further investigation into the detailed underlying mechanisms need to be carried out in future studies.
Investigating the kinase or transcription factor pathways involved in morphine-induced kidney injury may provide insight into new potential targets for therapy. STAT3, AKT and mitogen-activated protein (MAP) kinases, including ERK1/2, JNK, and p38, have been implicated in podocyte injury and the progression of chronic kidney diseases (CKD) [6], [14], [24], [25], [26]. All these kinases or transcription factors may also be activated by morphine in various cells [6], [30], [31]. We examined the effect of morphine on the activation of these kinases and factors in podocytes. Our results revealed that morphine stimulated the phosphorylation of AKT, JNK and p38 (Figure 10), but could not activate Erk1/2 or STAT3 (data not shown). These observations were further confirmed by the use of KOR and MOR specific agonists on human podocytes in morphine milieu (data not shown).
Takano et al reported that activation of AKT suppressed the expression of nephrin [32]; Ikezumi et al claimed that activation of JNK or p38 decreased the expression of nephrin and podocin, while inhibiting these kinases restored their expressions [33]. Consistent with these reports, we also found that inhibiting AKT, JNK, and p38 could partially prevent the morphine-induced decrease of SDCMs (Figure 11), indicating the regulation of these three kinases in morphine-induced SD damage.
In conclusion, we have demonstrated that morphine has the potential to directly impair the SDCMs in podocytes, which will contribute to acute kidney injury. The effects of morphine on podocytes are mediated through both MOR and KOR. These impairments are through generation of ROS, and are regulated by AKT, JNK, and p38 pathways. Our study provides insight into new mechanisms involved in morphine-induced podocyte damage, and highlights some new therapeutic targets for morphine induced kidney injury.
Author Contributions
Conceived and designed the experiments: XL MAS PCS RL PWM. Performed the experiments: XL NC PR RL PWM. Analyzed the data: MH KC AM JTC KG. Contributed reagents/materials/analysis tools: MAS PWM. Wrote the paper: XL PCS.
- 1. MacPherson RD (2000) The pharmacological basis of contemporary pain management. Pharmacol Ther 88: 163–185.
- 2. Dasgupta N, Kramer ED, Zalman MA, Carino S Jr, Smith MY, et al. (2006) Association between non-medical and prescriptive usage of opioids. Drug Alcohol Depend 82: 135–142.
- 3. Hopfer CJ, Mikulich SK, Crowley TJ (2000) Heroin use among adolescents in treatment for substance use disorders. J Am Acad Child Adolesc Psychiatry 39: 1316–1323.
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Nielsen DA, Kreek MJ (2012) Common and specific liability to addiction: Approaches to association studies of opioid addiction. Drug Alcohol Depend Suppl 1: S33–41.
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- 6. Weber ML, Farooqui M, Nguyen J, Ansonoff M, Pintar JE, et al. (2008) Morphine induces mesangial cell proliferation and glomerulopathy via kappa-opioid receptors. Am J Physiol Renal Physiol 294: 1388–1397.
- 7. Mongia A, Bhaskaran M, Reddy K, Manjappa N, Baqi N, et al. (2004) Protease inhibitors modulate apoptosis in mesangial cells derived from a mouse model of HIVAN. Kidney Int 65: 860–870.
- 8. Patel J, Manjappa N, Bhat R, Mehrotra P, Bhaskaran M, et al. (2003) Role of oxidative stress and heme oxygenase activity in morphine-induced glomerular epithelial cell growth. Am J Physiol Renal Physiol 285: F861–869.
- 9. Singhal PC, Sagar S, Reddy K, Sharma P, Ranjan R, et al. (1998) HIV-1 gp120 envelope protein and morphine-tubular cell interaction products modulate kidney fibroblast proliferation. J Investig Med 46: 243–248.
- 10. Singhal PC, Sharma P, Sanwal V, Prasad A, Kapasi A, et al. (1998) Morphine modulates proliferation of kidney fibroblasts. Kidney Int 53: 350–357.
- 11. Bourgoignie JJ (1990) Renal complications of human immunodeficiency virus type 1. Kidney Int 37: 1571–1584.
- 12. Rao TKS, Filppone EJ, Nicastri AD, Landesman H, Frank E, et al. (1984) Associated focal segmental glomerulosclerosis in the acquired immunodeficiency syndrome. N Engl J Med 310: 664–673.
- 13. Johnson JE Jr, White JJ Jr, Walovitch RC, London ED (1987) Effects of morphine on rat kidney glomerular podocytes: a scanning electron microscopic study. Drug Alcohol Depend 19: 249–257.
- 14. Benzing T (2004) Signaling at the slit diaphragm. J Am Soc Nephrol 15: 1382–1391.
- 15. Mao J, Zhang Y, Du L, Dai Y, Yang C, et al. (2006) Expression profile of nephrin, podocin, and CD2AP in Chinese children with MCNS and IgA nephropathy. Pediatr Nephrol 21: 1666–1675.
- 16. Xing Y, Ding J, Fan Q, Guan N (2006) Diversities of podocyte molecular changes induced by different antiproteinuria drugs. Exp Biol Med (Maywood) 231: 585–593.
- 17. Husain M, Meggs LG, Vashistha H, Simoes S, Griffiths KO, et al. (2009) Inhibition of p66ShcA longevity gene rescues podocytes from HIV-1-induced oxidative stress and apoptosis. J Biol Chem 284: 16648–16658.
- 18. Salhan D, Sagar A, Kumar D, Rattanavich R, Rai P, et al. (2012) HIV-associated nephropathy: role of AT2R. Cell Signal 24: 734–741.
- 19. Nistala R, Whaley-Connell A, Sowers JR (2008) Redox control of renal function and hypertension. Antioxid Redox Signal 10: 2047–2089.
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Susztak k, Raff AC, Schiffer M, Böttinger EP (2006) Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy. Diabetes 55: 225– 233.
- 21. Marshall SM (2007) The podocyte: a potential therapeutic target in diabetic nephropathy? Curr Pharm Des 13: 2713–2720.
- 22. Sharp BM, Keane WF, Suh HJ, Gekker G, Tsukayama D, et al. (1985) Opioid peptides rapidly stimulate superoxide production by human polymorphonuclear leukocytes and macrophages. Endocrinology 117: 793–795.
- 23. Singhal PC, Pamarthi M, Shah R, Chandra D, Gibbons N (1994) Morphine stimulates superoxide formation by glomerular mesangial cells. Inflammation 18: 293–299.
- 24. Husain M, Sunamoto M, D'Agati VD, Klotman ME, Iyengar R, et al. (2004) Nef stimulates proliferation of glomerular podocytes through activation of Src-dependent Stat3 and MAPK1, 2 pathways. J Clin Invest 114: 643–651.
- 25. Koshikawa M, Mukoyama M, Mori K, Suganami T, Sawai K, et al. (2005) Role of p38 mitogen-activated protein kinase activation in podocyte injury and proteinuria in experimental nephrotic syndrome. J Am Soc Nephrol 16: 2690–2701.
- 26. Lim AK, Nikolic-Paterson DJ, Ma FY, Ozols E, Young MJ, et al. (2009) Role of MKK3-p38 MAPK signalling in the development of type 2 diabetes and renal injury in obese db/db mice. Diabetologia 52: 347–358.
- 27. Huber TB, Benzing T (2005) The slit diaphragm: a signaling platform to regulate podocyte function. Curr Opin Nephrol Hypertens 14: 211–216.
- 28. Dzau VJ (2001) Theodore Cooper Lecture: Tissue angiotensin and pathology of vascular disease: A unifying hypothesis. Hypertension 37: 1047–1052.
- 29. Tojo A, Asaba K, Onozato ML (2007) Suppressing renal NADPH oxidase to treat diabetic nephropathy. Expert Opin Ther Targets 11: 1011–1018.
- 30. Ma W, Zheng WH, Powell K, Jhamandas K, Quirion R (2001) Chronic morphine exposure increases the phosphorylation of MAP kinases and the transcription factor CREB in dorsal root ganglion neurons: an in vitro and in vivo study. Eur J Neurosci 14: 1091–1104.
- 31. Wen H, Lu Y, Yao H, Buch S (2011) induces expression of platelet-derived growth factor in human brain microvascular endothelial cells: implication for vascular permeability. PLoS One 6: e21707.
- 32. Takano Y, Yamauchi K, Hayakawa K, Hiramatsu N, Kasai A, et al. (2007) Transcriptional suppression of nephrin in podocytes by macrophages: roles of inflammatory cytokines and involvement of the PI3K/Akt pathway. FEBS Lett 581: 421–426.
- 33. Ikezumi Y, Suzuki T, Karasawa T, Kawachi H, Nikolic-Paterson DJ, et al. (2008) Activated macrophages down-regulate podocyte nephrin and podocin expression via stress-activated protein kinases. Biochem Biophys Res Commun 376: 706–711.


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Amanda Todd We Day Vancouver: Demi Lovato, Desmond Tutu, Magic Johnson Discuss Bullying, Inspire Youth
Bullying was top of mind at We Day Vancouver, the annual event to inspire young people to make positive change, as celebrities and politicians cited B.C. teen Amanda Todd as an example of the devastation it causes.
Todd, 15, killed herself last week after years of bullying, and after she shared her tormented experience on YouTube. Todd had planned to get a "Stay Strong" tattoo just like singer Demi Lovato, reported the Vancouver Sun.
Lovato, who spoke at We Day on Thursday, is no stranger to bullying. She's said that her middle-school classmates organized "hate petitions," smashed her demo CDs and heckled her across the street from her house.
"I know there's someone out here in this audience who's being bullied and I know they need someone to look up to," Lovato told the Vancouver audience of 20,000.
"This is an issue that we can beat. This is something that you can get through, and this is something that will not bring you down," she said. "I want you to help spread awareness of bullying and how serious it is."
B.C. Premier Christy Clark took the stage in pink, directly addressing Todd's legacy.
"She felt people's fists, and she felt their words. And Amanda left us a video record of what it felt like," Clark said. "She needed someone to stand up, to move from 'me to we.' To stand up and not be a bystander."
Story continues after slideshow:
Archbishop Desmond Tutu, a central figure in ending apartheid in South Africa, high fives a girl in Vancouver.
Archbishop Desmond Tutu laughs while being interviewed by Josh Ostoff, music editor for The Huffington Post Canada.
Christy Clark speaks at We Day Vancouver 2012.
B.C. Premier Christy Clark speaks at We Day Vancouver 2012.
Miss Teen World Canada 2012 speaks at We Day Vancouver 2012.
Miss Teen World Canada 2012 speaks at We Day in Vancouver.
How some youth in the audience are experiencing We Day Vancouver 2012.
The Vancouver mayor speaks at We Day Vancouver 2012.
Vancouver's mayor speaks at We Day Vancouver 2012.
Two We Day hosts lead a crowd of thousands in a dance.
We Day Vancouver 2012 host Jesse Giddings.
Marc and Craig Kielburger are the founders of Free The Children.
Craig Kielbuger is a co-founder of Free The Children.
Vancouver Canucks COO Victor De Bonis speaks at We Day 2012.
Clark encouraged the crowd to stand up like Todd and Terry Fox, the runner from Port Coquitlam who lost a leg to cancer but still ran across the country to raise money for cancer research.
"Stand up! Stand up! Be that person every day, when a vulnerable person is at risk," she said.
The premise behind We Day is simple: pack a ton of youth into a stadium setting and provide a forum for them to hear motivating messages from celebrities and politicians. Students are told about how they can work with charities or become "social entrepreneurs," which involves using business tools to create change.
We Day is hosted by Free the Children, a global charity run by Canadian brothers Craig and Marc Kielburger. Students who attend We Day earn their entry by volunteering and raising money for various non-profit groups.
We Day promotes ongoing campaigns to install water pumps and wells overseas, end hunger in Canada, and raise awareness of aboriginal issues, child labour and female oppression.
Spencer West found his own way of fighting back against bullies. West, who had his legs amputated when he was five years old due to a genetic disorder, climbed Mt. Kilimanjaro with his hands.
"When we were getting to the top [of Mt. Kilimanjaro] what was really going through my mind was this is for anyone who has ever been bullied," said the Toronto man at We Day.
"We made it to the top. This is for anyone who has ever felt powerless, for anyone who has ever wanted to make a change in the world."
Basketball star Magic Johnson kept on with the same theme. He told the audience that discrimination is "not a cool thing."
"When you're a leader and you see someone getting bullied, a leader steps in and stops that," he said.
Archbishop Desmond Tutu, a social rights activist who chaired South Africa's Truth and Reconciliation Commission, told a We Day news conference that there is no problem in the world that can't be solved.
"There was a time when people told us, apartheid would never end, or if it was going to end, it was going to end with a racial bloodbath. It's ended!" he said.
"There was a time that people thought that all of the oppression in the Soviet Union would never end. It's ended! So why shouldn't we say, this is a world that is made for goodness? That each one of us is made for goodness?"
Previous We Day events have included Justin Bieber, the Jonas Brothers, and Jennifer Hudson. Vancouver's We Day is one of eight in Canada this year.
Organizers announced on Thursday that We Day would be expanding to the United Kingdom.
At a We Day event in 2011, Victoria-born singer Nelly Furtado was so moved by her experience working with Free the Children in Kenya that she donated $1 million to the group. The money has gone to building an all-girls school.


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Can the Turkish opposition kick out Erdogan?
- By David KennerDavid Kenner is the Middle East editor at Foreign Policy. He is based in Beirut, Lebanon, and has been with FP since 2009 (a long time, he knows). He worked for FP previously in Cairo, where he covered the early days of the Arab Spring, and before that in Washington. He has attended Georgetown University and the American University of Beirut and has reported from Libya, Egypt, Gaza, Turkey, Lebanon, and Iraq.
- david.kenner
- @davidkenner
A few months back, I had a pleasant lunch at a Turkish restaurant in Dupont Circle with representatives of a nascent Turkish political party, TDH. The party billed itself as a Western-oriented alternative to the ruling AKP party — and also as more dynamic and forward-looking than the CHP, the opposition party that has been the traditional home of secular Turks. It turned out to be a short-lived venture: Today, party leader Mustafa Sarigul announced that he was abandoning his plans to establish TDH as an independent political party, and would throw his support behind the CHP is Turkey's 2011 general election.
Sarigul suggested that international and domestic developments — a reference to Prime Minister Erdogan's vociferous criticism of Israel in the wake of the Gaza flotilla disaster and the recent flare-up of Turkish-Kurdish tensions — were the reason TDH leaders had to "act as statesmen and unite" with opposition groups. The real reason, however, probably has more to do with changes in the CHP, and within Turkey's political climate. After the resignation of CHP leader Deniz Baykal following a sex scandal, his replacement, Kemal Kilicdaroglu, has mended bridges with Baykal's old rivals — including Sarigul.
Just as importantly, the Turkish opposition seems to have gained new life. Two recent polls found that the CHP was polling at its highest level in years, now receiving the support of approximately 30 percent of Turks. There are a variety of possible reasons for this improvement in the party's fortunes: the new leadership of Kilicdaroglu, Turkish anger that the AKP's much-celebrated "Kurdish opening" failed to achieve results, discontent over Erdogan's Middle East adventurism, and double-digit unemployment in a job market that still has not turned the corner following the international recession. Whatever the reason for the CHP's revival, its newfound strength makes it unlikely that there would be political space for a nascent party such as TDH to establish a foothold.
The 2011 election is still a year away — a lifetime in politics. But it looks like the Turkish opposition is going to enter campaign season unified and energized in a way that must have Erdogan sweating.


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Finally, Satisfaction - 2000 Ford Super Duty DieselPosted in Vehicle Reviews on July 1, 2006 Comment (0)
Shocking but true: There hasn't been a Ford Super Duty on the cover of Four Wheeler for almost two years. Well, it's high time we changed that Super Duty drought by presenting Ron and Christine Williams' stunning '00 Crew Cab shortbox.We met the Williamses at the Summer 4-Wheel Jamboree in Bloomsburg, Pennsylvania. We were impressed by the clean look of their massive Super Duty, but as we talked with them we became even more impressed as we learned that Ron did all of the project planning and almost all of the wrenching on the truck himself (with some help from friends including Barry Miller and Tony Lacy). All while he was overseeing his busy bathroom remodeling business.
Williams is no stranger to building custom trucks--he has built three project trucks previously--but just when he thought he "had his modifying withdrawal somewhat under control" he had the uncontrollable urge to build this Super Duty F-250 diesel. Of course the truck went through several significant alterations before he hit on the magic formula. Now that his truck is completed, Williams can honestly say that he's finally satisfied.
Don't look for this orange pearl metallic paint at your local paint supplier, because you won't find it. It was custom-mixed to Williams' specifications by the folks at Auto Body by Duie in Bordentown, New Jersey. They also applied the silver metallic tribal flames, which were then outlined in purple before the truck was covered in clearcoat. Even with the arresting paint, the overall look of the Crew Cab is simple and uncluttered, thanks to an absence of gaudy bolt-ons. Williams kept the look simple by adding only a set of Carr sidesteps, a Distinctive Truck front bumper cover, Reflexxions steel hood, Stull Industries outer grille shell and billet grille, and a Randy Ellis Designs Sleekster frame-mounted lightbar with four APC driving lights.
Helping to aim the moderately sized 42x14-16.5 Super Swamper IROK tires on 16.5x10 Weld Scorpio forged-aluminum wheels is an Off Road Unlimited crossover steering kit and a beefy 1.5-inch-diameter Bullet Proof Steering Rock Proof tie rod that features 0.75-inch chromoly Stage 2 spherical rod ends. Other steering mods include a Skyjacker drop pitman arm and a Fox Racing Shox 2.0 steering stabilizer. The Dana 50 axle retains its stock internals, but is protected by a Purple Cranium Half Spider differential rock guard.
Since Williams purchased the Super Duty in 2000, the truck has been fitted with four different suspension lifts. None fulfilled his desires until he permanently removed the leaf springs in favor of a Kelderman Air Ride 15-inch air suspension system. Williams says: "That was a lot of work, especially when your truck is too big for your garage and it rains almost every day." Here you can see some of the front suspension componentry, which includes dual Fox Racing Shox 2.0 remote-reservoir shocks.
Owner: Ron and Christine Williams, Windsor, New Jersey
Vehicle/Model: '00 Ford F-250 Super Duty
Estimated value: $50,000
ENGINEType: 7.3L V-8 diesel
Aspiration: Fuel injection, Superchips Max MicroTuner, Motorvation performance computer chip, custom 4-inch-diameter downpipe and dual exhaust Output, hp/torque @ rpm (estimated): 480 @ 4,000/680 lb-ft @ 3,000
Transmission: 4R100, Banks TransCommand Module
Transfer case: Stock with Attitude Performance reindexing ring and crossmember
Front: Kelderman Air Ride 15-inch-lift, dual Fox Racing Shox 2.0 remote-reservoir shocks
Rear: Kelderman Air Ride 15-inch-lift, Fox Racing Shox 2.0 remote-reservoir shocks
Front: Dana 50, ORU crossover steering, Bullet Proof Steering Rock Proof tie rod
Rear: Sterling 10.5-inch/open
Ring-and-pinion: 3.73:1
Wheels: 16.5x10 Weld Scorpio
Tires: 42x14-16.5 Super Swamper IROK


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Drywall for 24" oc
I'm about ready to drywall my basement, and I'm trying to decide what thickness of drywall to use. All of the exterior walls were framed by the builder at 24" on center, should I use 1/2 or 5/8 on these walls?
I'm pretty confused, because my house which is 2 years old has 24" walls all upstairs and they used 1/2", but from what I've read on the internet, you should use 5/8" 24" centers. Plus everyone I talk to at the big box stores thinks I'm crazy for asking about 5/8 for walls :huh:
5/8 all the way. i wouldn't use any 1/2 on any wall no matter what the spacing. i know some people use it but its too flimsy for me. don't listen to the people at the big box stores, most of them do not know what they're doing. (around here, NY).
1/2" is legal but as stated above is flimsy, spend the few extra cents and go 5/8" makes all the difference in the world.
I'm not sure what you mean by saying 1/2" is flimsy, it's been the standard thickness, especially for 16 oc construction for many years. I've never seen a wall crack or heave (without something being put through it).
I can see how sagging could be a problem for ceilings (I'm doing 5/8 on the ceiling anyway). But what potential issues could I have with 1/2" on my 24" oc walls (which are also filled with batt insulation if that matters)?
7 bucks difference between 1/2 and 5/8 drywall? WOW. it's under a dollar difference in wisconsin. I have always used 1/2 for walls in houses and always space studs 16 O.C. I haven't seen anything framed 24's in a long time and don't have any experience with drywall on them. I would think if your happy with the combo of 24's and 1/2 drwall upstairs it should be fine downstairs. Just my opinion since i don't have any experience with your situation.
1/2" board on 24" centers will feel spongy to anyone who leans on them, not that this is usually a problem, but if someone were to fall hard enough it's going to leave a mark (crack). 5/8 is substantially stronger and will also float better on the studs preventing any waving between the studs as will be inherant in 1/2. 24" centers are a long span for 1/2" I wouldn't do it if it were my house.
Shop around for the drywall, cost difference is usually 1.5 to 3 cents per sq.ft. more for 5/8
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This technology, developed at Vanderbilt University's Institute for Software Integrated Systems, uses radio interferometry to locate tangible objects and attains, simultaneously, a higher degree of accuracy (within 3 centimeters), considerably longer range (up to 160 meters) and lower cost than other technologies.
Challenges in sensor localization for wireless sensor networks (WSN)
- Most WSN applications require the knowledge of the location of individual nodes that form the network
- Although GPS and acoustic ranging are used for this, robust sensor localization is still an unresolved problem
- GPS technologies are relatively expensive and not accurate enough for many applications, whereas acoustic ranging technologies are more accurate, but have limited range
- Ultrasound, the only option for applications that require stealth, have limited range and directionality constraints
- Traditional radio based methods typically rely on received signal strength that is relatively accurate over short distances (with extensive calibration), but are imprecise beyond a few meters and are very expensive
Technology Description
The novel idea behind the proposed technology is that it utilizes two simple transmitters to create the interference signal directly. If the frequencies of the two emitters are almost the same, the composite signal will have a low frequency envelope that can be measured by modestly priced and simple hardware readily available on a WSN node. Relative phase offset of the signal at two receivers is then used, which is a function of the relative positions of the four nodes involved and the carrier frequency. By making multiple measurements in a network that contains a minimum of seven nodes, it is possible to reconstruct the relative location of the nodes in three dimensions.
Commercial Applications
In addition to wireless sensor networks, this technique is equally relevant to any application that requires highly accurate, three dimensional positioning including structural engineering, sports and robotics.
Intellectual Property and Product Development Status
- U.S. Patent number 7,558,583
- Prototype implementation runs on the XBow MICA2 platform and yields an average localization error as small as three centimeters and a range of up to 160 meters
- Vanderbilt collaborated with the Oak Ridge National Laboratory to showcase this technology during a "Dirty Bomb Detection and Localization" demonstration at the Vanderbilt stadium. This technology was used to track a plain clothes security guard who circulated through the stadium with a cell phone-integrated radiation detector both on a television monitor and on a Google Earth™ user-interface
- More details and Demonstration videos are available at: http://www.isis.vanderbilt.edu/projects/rips


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LG Electronics USA pre-leases distribution facility at Heritage Crossing Corporate Center
March 22, 2016 | Staff Writer | Print Article | Email this Article
LG Electronics USA has pre-leased a 363,224-square-foot freestanding distribution facility at Heritage Crossing Corporate Center, 14746 South Gougar Road in Lockport, Illinois.
The building, currently under construction, is slated for completion in summer 2016. It is the first 36-foot clear facility along Interstate 355.
Buildings at Heritage Crossing Corporate Center range from 60,000 square feet to 521,265 square feet. The 228-acre business park is located in Lockport, Illinois, situated directly at the Interstate 355 and 143rd Street interchange.
James Frank and Jason West of Cushman & Wakefield represented LG Electronics, and Dan Leahy, Mark Moran, and John Whitehead of NAI Hiffman represented ML Realty Partners in the transaction.
Tags | Cushman & Wakefield, Heritage Crossing Corporate Center, LG Electronics USA
© 2017 Real Estate Communications Group. Duplication or reproduction of this article not permitted without authorization from the Real Estate Publishing Group. For information on reprint or electronic pdf of this article contact Mark Menzies at 312-644-4610 or email@example.com


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Miguel – Adorn Review
"Adorn" is performed by American recording artist Miguel, and taken from his second studio album Kaleidoscope Dream. It was released on 7 August, 2012 by RCA Records as the first single from the album. It was produced and written by Miguel. It has received 3 nominations at the 55th Grammy Awards in the category of "Song of the Year", "Best R&B Performance" and "Best R&B Songs".
"Adorn" ranked at number 1 on the US Billboard Hot R&B/Hip-Hop Songs Chart, at number 35 on the US Billboard Pop Songs Chart, at number 17 on the US Billboard Hot 100 Chart, at number 173 on the UK Singles Chart and at number 187 on the French SNEP Chart.


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Follower's Drag to Left
Left-to-right contact
Lady's foot stays connected
with man's
DT: Andrew, how does she know to keep contact with your feet?
Andrew: Again, mainly through our frame. We keep slight pressure against her foot and pivoting the follower to take a back step keeps her foot in that position. As the leader continues to turn, she will drag our foot.
DT: Would an experienced follower be able to follow that on the social dance floor.
Andrew: Oh, yes, yes.
DT: Kana, that's an easy lead and follow?
Kana: Um hm. Ladies often ask what to do after the first drag. Unless you are feeling some kind of a clear stop from the leader, after foot is dragged, ladies always try to close your feet. In this figure, after you are dragged to the different positions you stand up and then close your feet.
DT: Close the feet?
Kana: After he drags the lady's foot, I am trying to close my feet so my left foot is touching my right foot.
DT: So, ideally, should the leader in your mind wait until your feet are touching before he initiates the next movement.
Kana: No, he's already initiating the next move. So in the course of one point to the other, I don't go from open foot to open foot. I always close my foot.
DT: Andrew, at the finish of the video, you invite Kana to step across. How did she know not to do that sooner, or, what kept her from doing that sooner?
Andrew: With correct frame, we keep her weight going towards her back foot. At the end of the sequence, we adjust our frame and change her direction and ask her to pivot and step across.
DT: Instead of leading the lady across at the end here, could you invite the lady to continue in a left molinete?
Andrew: We could. We just pull our left leg away and continue to turn towards our left and she would do a grapevine or molinete.
DT: Any other possibilities come to mind other than inviting her across and left molinete?
Andrew: Sure, we could hold her in that position and make a sandwich and step back left and then she would cross over our right foot. Another option is to walk out to the cruzada or cross. There are numerous options, but those two come immediately to mind.
DT: Main points?
Andrew: Because we are doing this repetitively, the leader needs to pivot on the right foot and be clear and as the follower takes our foot we are pivoting to our left on our right foot and the follower is taking our left foot to the side. We pivot and our leg stays rigid in our hip. Our leg does not go out to the side.
DT: The lady is not turning the man, the man is turning himself?
Andrew: Exactly, exactly.
Some Keys:Absent a parada or stop, the lady's next step after a drag is to close her feet. In this figure, perhaps even more than in others, the leader has to keep the lady in position so as to continue the foot to foot contact.
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Motorola WX180 pinouts
The connector/pinout information provided here is applicable to the following device:
Manufacturer: Motorola
Manufacturer: Motorola
Device name: WX180
There is one pinout found for Motorola WX180.The pinout (pin-out) is a cross-reference between the contacts (pins) of an electrical connector and their functions. The link to apprpiate pinout shown as a table or diagram are listed below.
- Mini-USB charging cable pinout suitable for a wide variety of devices


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- Category: Teen
- Views: 24,837
- Added: 6 years ago
- Description: My exgirlfriend is such a whore...she was fucking my best friend while I was out of town. This is our homemade video for my birthday when we used to date
- Tags: blowjob, facial, big, tits, ass, brunettes
- Pornstars: Add Pornstar
- Favorite by: 52
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