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BioMed Research International
Volume 2013 (2013), Article ID 459196, 8 pages
Vasculoprotective Effects of Combined Endothelial Progenitor Cells and Mesenchymal Stem Cells in Diabetic Wound Care: Their Potential Role in Decreasing Wound-Oxidative Stress
1Inter-Department of Physiology, Graduate School, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
2Stem Cell and Cell Therapy Research Unit, Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
3King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand
4Department of Physiology, Center of Excellence for Microcirculation, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
Received 9 March 2013; Revised 10 May 2013; Accepted 15 May 2013
Academic Editor: Dimitrios P. Bogdanos
Copyright © 2013 Supakanda Sukpat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
To investigate whether the combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) could enhance angiogenesis and wound healing in diabetic mice. Balb/c nude mice were divided into five groups, including a control group, diabetic group (DM), DM injected with 1 × 106 cells MSCs, DM injected with 1 × 106 cells EPCs, and DM injected with combined 0.5 × 106 cells MSCs and 0.5 × 106 cells EPCs. After seven weeks, the mice were anesthetized, and bilateral full-thickness excision skin wounds were made on the dorsorostral back. The percentage of wound closure in DM group decreased significantly than in control and all other treated groups on day 7 and day 14 (). On day 14, the percentage of capillary vascularity in combine-treated group was significantly higher than in DM (). In the present study, we have demonstrated that the combined EPCs and MSCs can increase vascular endothelial growth factor (VEGF) level and angiogenesis which resulted in reduced neutrophil infiltration, decreased malondialdehyde (MDA) levels, and enhanced wound healing in diabetic mice model.
1. Introduction
A diabetic wound is characterized by poor circulation and impaired angiogenesis, which contributes to nonhealing ulcers and may result in subsequent amputation. In addition, 84% of amputations among diabetic foot-ulcer patients have been reported [1–3].
Diabetes-induced endothelium and vascular dysfunction are closely associated with oxidative stress due to hyperglycemia caused by the excessive generation of reactive oxygen species (ROS). It has also been suggested that ROS can reduce the number and function of EPCs [4]. Consequently, EPC dysfunction may decrease the vascular regenerative potential of diabetic patients and thereby contribute to the pathogenesis of diabetic ulcers [4]. Moreover, high levels of superoxides in the diabetic wound may result in abnormalities in the number of local cytokines in the wound as well as reduce the production of growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), and vascular endothelial growth factor (VEGF), which contribute to the delay in wound healing [5].
Recently, several studies have suggested that MSCs can promote wound healing, angiogenesis, and the release of proangiogenic factors, including VEGF, basic fibroblast growth factor (bFGF) and stromal-derived factor-1 (SDF-1) [6–8]. Thus, this study aimed to examine the effect of combined EPCs and MSCs treatment on angiogenesis and wound healing in diabetic mice. In particular, we assessed the levels of ROS in the wound area where the EPCs and MSCs were injected. We hypothesized that the vasculoprotective effect of these combined EPCs and MSCs may be due to their ability to decrease ROS levels in the diabetic wound area.
2. Materials and Methods
2.1. Animal Preparation
Male Balb/c nude mice (7-8 weeks old, 20–25 g) were purchased from the National Laboratory Animal Center, Salaya Campus, Bangkok, Thailand. The experimental procedures were conducted according to the guideline of experimental animals by The National Research Council of Thailand and were approved by the ethics committee at the Faculty of Medicine, Chulalongkorn University. The mice were housed at room temperature (°C) with one mouse per cage and fed with standard chow and sterilized water. The mice were divided into five groups. Group 1: wound control group with implanted fibrin gel (control). Group 2: diabetic wound group with implanted fibrin gel (DM). Group 3: diabetic wound group with implanted fibrin gel and 1 × 106 MSCs (DM + MSCs). Group 4: diabetic wound group with implanted fibrin gel and 1 × 106 EPCs (DM + EPCs). Group 5: diabetic wound group with implanted fibrin gel and combined 0.5 × 106 EPCs and 0.5 × 106 MSCs (DM + EPCs + MSCs).
Animals in the diabetic groups were induced by an injection of streptozotocin (STZ, Sigma Chemical Co., USA) in citrate buffer, pH 4.5 (Sigma Chemical Co., USA) at a dose of 45 mg/kg i.p. daily for 5 days. Two weeks later, the glucose level was measured from tail-vein blood, and mice with glycemia (over 200 mg/dL) were selected for the study [9].
2.2. Preparation of Mouse MSCs
Mouse MSCs were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 5% fetal bovine serum (FBS), 1x Pen-strep, and L-glutamine at 37°C, 5% CO2. The medium was changed every 2 or 3 days. The cells were maintained by the Stem Cell and Cell Therapy Laboratory.
2.3. Preparation of Human EPCs
Human EPCs were purchased from Lonza. The cells were plated on culture dishes coated with fibronectin (Gibco) and cultured in endothelial cell basal medium 2 (EBM-2, Lonza) at 37°C and 5% CO2. The medium was changed every 2 or 3 days. The cells were maintained by the Stem Cell and Cell Therapy Laboratory.
2.4. Preparation of Stem Cells and Fibrin Gel
The cells were washed with sterile phosphate-buffered saline (PBS) and then incubated with 2 mL 0.25% trypsin for 1 minute at 37°C. Next, 10 mL of culture medium was added to the plate to neutralize the trypsin activity. The cell suspension was transferred into a tube, and the number of cells was quantified using a microscope at 100x magnification. The cells were then centrifuged for 5 min at 1000 rpm at room temperature. The supernatant was removed, and the cell pellet was mixed with 30 μL fibrinogen and 30 μL thrombin per wound (the fibrin gel consisted of fibrinogen and thrombin and was obtained from Shanghai RAAS Blood Products Co. Ltd, China) [10].
2.5. Wound Model
Six to seven weeks after the streptozotocin injection, the mice were anesthetized by an intraperitoneal injection of sodium pentobarbital at a dose of 55 mg/kg and an alcohol swab was used on its dorsorostral back. Bilateral full-thickness excision skin wounds (0.6 × 0.6 cm2) were created on each side of the midline [8]. Each mouse received fibrin gel (control and DM), fibrin gel and 1 × 106 MSCs (DM + MSCs), fibrin gel and 1 × 106 EPCs (DM + EPCs), and fibrin gel with combined 0.5 × 106 MSCs and 0.5 × 106 EPCs (DM + EPCs + MSCs) on the wound bed. The wounds were then covered using Tegaderm (3M, USA). The EPCs and MSCs were maintained by the Stem Cell and Cell Therapy Research Unit at Chulalongkorn University. In this study, we would like to test the effect of human EPCs on diabetic wound (DM + EPCs group) and to compare with another reference that used only mouse MSCs [8], and also with the combined group. Therefore, we need to develop the diabetic model in nude mice, which demonstrate an immune deficiency.
2.6. Wound Analysis
Digital images of the wounds were taken at days 0, 7 and 14. The wound area was analyzed using digital image software analysis (Image Pro II 6.1). The percentage of wound closure (%WC) was calculated using the following equation: (area of the original wound − area of the actual wound)/area of the original wound × 100 [8].
2.7. Determination of Capillary Vascularity
At days 7 and 14 following wounding, the mice were anesthetized with an intraperitoneal (IP) injection of sodium pentobarbital at a dose of 55 mg/kg. The jugular vein was cannulated for injection of 0.2 mL of 5% FITC-labeled dextran (MW = 250000, Sigma Chemical Co., USA). The percentage of capillary vascularity (CV) was examined using intravital fluorescence video microscopy with a 10x objective lens [11]. From the video images, the percentage of capillary vascularity (%CV) was analyzed using Image Pro II 6.1 software. The region of interest (ROI) was 100 × 100 pixels. Each ROI was selected to cover only capillary networks with a diameter of less than 15 μm [11, 12]. The average CV consisted of at least three ROIs from each animal.
These results were confirmed by a blind assessment. The %CV was calculated using the following formula: (number of pixels within the capillaries/total number of pixels within the select area) × 100 [11, 12].
2.8. Measurement of Neutrophil Infiltration
The mice were sacrificed at 7 and 14 days, and the wound samples were harvested at a size of 0.6 × 0.6 cm2. The tissue specimens were fixed in 10% formaldehyde for 24 hours. The center of the wound samples was cut and embedded in paraffin. Four-micrometer thick sections were stained with hematoxylin-eosin (H&E). Neutrophil infiltration was measured using a microscope (Nikon E50i, Japan) at 400x magnification. The inflammatory wound area of the specimens was evaluated. The wound image was captured by a digital camera (Nikon DS-L2, Japan), and the amount of neutrophil infiltration was analyzed using Image Pro II 6.1 software [12]. These results were also confirmed by blind assessment.
2.9. Determination of Tissue VEGF, Malondialdehyde Levels, and Total Tissue Protein
The tissue sample was harvested from each mouse at 7 and 14 days following wounding and frozen at −80°C. A 50 mg tissue sample was homogenized in 50 mL RIPA lysis buffer (Cell Signaling Technology, Inc.) with protease inhibitor (Sigma Chemical Co., USA) and phosphatase inhibitor cocktails (Sigma Chemical Co., USA). The sample was sonicated and centrifuged at 10,000 rpm for 10 min. The supernatants of each tissue sample were used to analyze the levels of VEGF, malondialdehyde (MDA; lipid peroxides parameter), and the total tissue protein using ELISA (R&D Systems), TBARS assay kit (Cayman Chemical Co., USA) and microplate BCA protein assay kit (Thermo Scientific, USA), respectively. The VEGF and MDA levels were expressed as pg/mg protein and nM/mg protein, respectively.
2.10. Statistical Analysis
All of the data were presented as the mean ± SEM, and comparison between groups was conducted using one-way ANOVA, followed by the Least Significant Difference posttest (LSD). The correlation analysis was conducted using the two-tailed Pearson's correlation. Differences were considered statistically significant at a value less than 0.05 ().
3. Results and Discussion
The results of body weight and blood glucose are shown for each group (Table 1). On days 7 and 14, the blood sugar in the DM, DM + MSCs, DM + EPCs, and DM + EPCs + MSCs groups were significantly higher than their age-matched controls. The body weights of the all four groups of diabetes tend to decrease; however, they were not shown to be of statistical significance; that may be due to the small number of each group and high variability (Table 1).
3.1. Effects of EPCs and MSCs on Wound Closure
On day 7 and day 14 of postoperative wound, the percentage of wound closure (WC) in all of the treatment groups and control group was significantly increased compared to the DM group (; Figure 1). Interestingly, 14 days after wounding, the %WC of the DM + EPCs group was significantly increased compared to the DM + MSCs and DM + EPCs + MSCs groups (; Figure 1).
3.2. Effects of EPCs and MSCs on Angiogenesis
The results on day 7 showed that the %CV in all of the treatment groups and control group was significantly increased compared to the DM group (; Figure 2). Moreover, these results indicated that, on day 7 and day 14, the %CV of the DM + EPCs + MSCs group was significantly higher compared to the values of the DM and DM + MSCs groups (, ; Figure 2).
3.3. Effects of EPCs and MSCs on Tissue VEGF Levels
The tissue VEGF levels of all of the treatment groups and control group were significantly increased compared to the DM group only on day 7 (Figure 3). There was no significant difference in the tissue VEGF levels among the groups on day 14.
3.4. Effects of Combined EPCs and MSCs on Neutrophil Infiltration
The number of neutrophils infiltrating into the wound area between the five groups is shown in Figure 4. On day 7, the number of infiltrating neutrophils in the DM group significantly increased compared to the control group (). Moreover, the DM + MSCs, DM + EPCs, and DM + EPCs + MSCs groups showed no significant difference when compared to the DM group (Figure 4).
On day 14, the number of infiltrating neutrophils in the DM group significantly increased compared to the control group (). The DM + MSCs, DM + EPCs, and DM + EPCs + MSCs groups showed a significantly reduced number of neutrophils compared to the DM group (, , , resp.); however, there was no significant difference among the DM + MSCs, DM + EPCs, and DM + EPCs + MSCs groups (Figure 4).
3.5. Effects of Combined EPCs and MSCs Treatment on Tissue MDA Levels
On day 7 and day 14 of postoperative wound, the tissue MDA levels in the DM group were significantly higher compared to all of the other groups (Figure 5). Interestingly, at 7 days after wounding, the tissue MDA levels in the DM + EPCs + MSCs group were significantly decreased compared to the DM and DM + MSCs groups (, ; Figure 5). However, there was no significant difference between the DM + EPCs + MSCs and DM + EPCs groups.
In the present study, to induce type 1 diabetes mellitus in nude mice, multiple low doses of STZ (45 mg/kg body weight) were administered to the mice daily for 5 days. STZ chemically damages pancreatic β cells by DNA damage and results in hypoinsulinemia and hyperglycemia [13, 14]. As shown in the present study, the blood glucose levels in the DM, DM + MSCs, DM + EPCs, and DM + EPCs + MSCs groups were significantly higher than those in the controls for both of the studied periods (Table 1). These results also showed no effect of EPCs and MSCs on the changes in blood glucose levels.
Diabetes is characterized by the reduced production of growth factors, poor circulation, and impaired angiogenesis, which contribute to poor wound healing, which has been reported in diabetic patients. VEGF, an important growth factor that induces angiogenesis, was also reduced in the DM group [15]. The present results showed that on day 7, tissue VEGF levels were significantly increased in the DM + MSCs, DM + EPCs and DM + EPCs + MSCs groups compared to the DM group. However, no significant difference between all of the groups on day 14 was observed. According to the normal process of wound healing, increased VEGF is normally increased maximally on day 7 and then declines at day 14 after the emergence of neocapillaries [16].
Taken together, these results also suggested that combined EPCs and MSCs have the potential to increase VEGF similar to the DM + EPCs and DM + MSCs groups. There was also no significant difference among the treatment groups on day 7.
It is thought that MSCs can increase chemoattractive and mitogenic factors, including VEGF, similar to EPCs [6–8]. Moreover, it has been reported that MSCs may also increase the number of cells positive for CD34, C-kit, or Flk-1, which are endothelial lineage cell markers and increase the recruitment of endothelial cells and endothelial progenitor cells into the wound [17–21]. As shown in our study (Figure 2), the combined DM + EPCs + MSCs group significantly demonstrated the highest increase in capillary vascularity on both day 7 and day 14. This result may be the first in vivo evidence indicating the synergistic effect of these two cell types on enhancing angiogenesis in the diabetic wound.
The present study also demonstrated that, on day 14, the number of infiltrating neutrophils in the DM + MSCs, DM + EPCs, and DM + EPCs + MSCs groups was decreased compared to the DM group (Figure 4).
Taken together, the results of the present study indicated that the combined treatment of EPCs and MSCs could reduce neutrophil infiltration, improve angiogenesis, and increase VEGF levels, which could promote a better outcome of wound closure in a diabetic mice model.
These results indicated that tissue MDA levels in the DM group were significantly increased compared to the control group. Combined EPC and MSC treatment significantly demonstrated the best potential in reducing the extent of wound-induced oxidative stress on day 7. The increase in ROS is a common characteristic at the wound site. In a previous study performed using an excision wound mouse model, the major product of lipid peroxidation, 4-hydroxy-2-nonenal (4-HNE), was reported [22]. Interestingly, coimmunostaining revealed that 4-HNE mainly colocalized with neutrophils; thus, it was suggested that the respiratory burst of these inflammatory cells results in the production of superoxide, which in turn causes lipid peroxidation.
Interestingly, low levels of ROS are required for the defense against invading pathogens [23], as well as for mediating intracellular signaling [24]. However, excessive amounts of ROS are deleterious due to their high reactivity.
These results suggested that combined EPC and MSC treatment could improve the wound healing process due to their consequential effects on angiogenesis and anti-inflammation, resulting in a decrease in antioxidative stress. We proposed that combined EPC and MSC treatment could inhibit diabetes-induced oxidative stress in the wound area via two potential mechanisms. The first mechanism involves the promotion of sufficient blood perfusion to enhance angiogenesis in the wound area by combined treatment with EPCs and MSCs. Thus, hypoxia cannot amplify the early inflammatory response, thereby inhibiting prolonged injury. The second mechanism is that combined EPC and MSC treatment inhibits oxidative stress produced from the inflammatory cells, thereby restoring the balance between ROS and the antioxidant capacity within the wound area.
Correlation analyses between tissue MDA levels and %CV and between MDA levels and the extent of neutrophil infiltration strongly support these two potential mechanisms (Figures 6 and 7). This correlation indicated that, in the treatment groups, the %CV increased with the reduced number of infiltrating neutrophils and significantly corresponded to the changes in wound ROS content, which is reflected at the MDA level.
Taken together, we showed that combined EPC and MSC administration significantly improved vascular function in diabetic wound mice. These data may have important implications for the potential use of MSCs as a cellular therapy and reduce the use of EPCs in diabetic wound conditions characterized by compromised vascularity and wound closure. Our study demonstrated that combined EPC and MSC treatment might provide benefits for diabetic patients and lower the risk of organ loss.
|Basic fibroblast growth factor
|Diabetes mellitus
|Dulbecco's Modified Eagle's Medium
|Endothelial cell basal medium 2
|Endothelial progenitor cells
|Fetal bovine serum
|Malondialdehyde levels
|Mesenchymal stem cells
|Phosphate-buffered saline
|Platelet-derived growth factor
|Region of interest
|Reactive oxygen species
|Stromal-derived factor-1
|Transforming growth factor-β
|Vascular endothelial growth factor
|Percentage of wound closure
|Percentage of capillary vascularity
This study was supported by the Ratchadapiseksompotch Fund of the Faculty of Medicine, the 90th Anniversary Research Fund, the Graduate School at Chulalongkorn University, Thailand, and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission (HR1166I). The authors have no conflict of interests to report.
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West Virginia authorities are slowly lifting a five-day ban on tap water, following the January 9, 2014 chemical spill into the Elk River.
January 16, 2014 by Hazmat Management
West Virginia authorities are slowly lifting a five-day ban on tap water, following the January 9, 2014 chemical spill into the Elk River.
Some 300,000 residents have been unable to drink, cook or even bathe with tap water since as much as 7,500 gallons of 4-methylcyclohexane methanol, also known as crude MCHM, spilled into the river.
West Virginia lies in the heart of coal country. The coal-cleaning chemical came leaked from a storage tank owned by Freedom Industries and located in Charleston, the state capital. The company has operated for some 22 years.
Residents began to complain that water smelled like licorice, which led authorities to investigate.
Freedom Industries President Gary Southern released the following statement after the discovery of the leak.
"Since the discovery of the leak, safety for residents in Kanawha and surrounding counties has been Freedom Industries' first priority," said Southern. We have been working with local and federal regulatory, safety and environmental entities, including the DEP, Coast Guard, Army Corp of Engineers and Homeland Security, and are following all necessary steps to fix the issue.
"Our team has been working around the clock since the discovery to contain the leak to prevent further contamination," added Southern.


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Crystal Township home damaged in fire, dog resuscitated
CRYSTAL TOWNSHIP — A modular home near Duck Lake was nearly destroyed in a Friday morning fire that required three fire departments to extinguish.
Crystal Township Fire Chief Lonny Fitzpatrick said the fire at 9231 E. Colby Road is believed to have started outside the home where a heat lamp over a dog kennel was too close to the siding. The fire call came in around 11:40 a.m.
"It got too close to the siding and then it went up the siding and into the eaves and then into the attic," Fitzpatrick said.
Firefighters from Crystal Township, Carson City and Richland Township fire departments were able to limit most of the severe damage to a bathroom and two bedrooms on one side of the home.
The owners, a couple in their late 20s according to Fitzpatrick, were not home at the time. Fitzpatrick did not know the names of the residents.
Two dogs were in the home's attached garage, but were saved by firefighters.
One of the pets, which had been exposed to heavy smoke, was resuscitated by a Crystal Township firefighter, who used his own gas mask to place over the dog's mouth for oxygen, according to Fitzpatrick. Montcalm County Emergency Medical Service personnel then used an oxygen mask to help save the dog.
Crews were on scene fighting the fire for about three hours.


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Tropical Delight
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Used to strengthen and moisturize
- 1 Cup coconut milk
- 1/4 Cup Honey
- 2 Tbsp Mango Butter/Oil
DirectionsMix ingredients. Warm slightly. Apply to clean damp hair. Wrap/cover for 30+ minutes. Rinse.
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English: Holme-next-the-Sea is a small village and civil parish in the English county of Norfolk. It is situated on the north Norfolk coast some 5 km north-east of the seaside resort of Hunstanton, 30 km north of the town of King's Lynn and 70 km north-west of the city of Norwich.
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- ► Holme Dunes National Nature Reserve (9 F)
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Moosheart Engagement Session from Hannah PerssonI love engagement sessions. I know, I know... I've said it a million times before, but, honestly, I can't help it. There's just something so magical about spending the day with your future Mr. or Mrs. and capturing all of that pre-wedding
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Theophilus Rose is found in the 1830 census of Nash County, North Carolin a. He has Three males under age 5, One male 5- 10, One male 20-30, One fem ale 5-10, One female 20-30, and One female 50-60.
Father: Theophilus Rose Sr. b: MAR 1762 in Unknown
Mother: Christiana b: 1765
Marriage 1 Patience Lamb\Lamm b: 1806 in Wayne County, North Carolina
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Red light for street repairs?
A plan to fund a street repair project was set in motion last week when Uvalde City Councilmen authorized advertising their intent to borrow up to $10 million; however, several city residents are already voicing their opposition. The notice of intent to borrow money, which outlines the plan to issue certificates of obligation, was published in this newspaper on July 10 and again today.
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West Seattle superstar to represent US at United World Games
By Gwen Davis
Did you know there is a United World Games for athletic youth? And that this year West Seattle's own 17-year-old Olivia Wake will represent the city and nation?
On June 15, Olivia, senior at Seattle Lutheran High School, will compete in volleyball in the 2014 United World Games in Klagenfurt, Austria.
She was cherry-picked by recruiters from the American International Sports Teams (AIST) to be on the U19 volleyball team. Female players from across the country were selected, including two others from Washington State. Around 13 female athletes were chosen.
The United World Games includes youth from 30 countries, for a range of sports. Players are bracketed according to their age. Olivia will participate in the bracket for 19-year-olds – the top of the age range.
"She got an invitation and it was an opportunity she just couldn't pass up," said Mark Wake, her dad.
Olivia is pumped.
"It's really exciting," she said. "I've never been to Europe or anything before. It will be fun to meet all the girls from other states, and get to know them and play with them."
"We are also going to be visiting Italy and Germany, and I'm excited to see those places, as well," she said. "I'm also looking forward to actually playing against other countries."
Olivia started playing volleyball in 7th grade. She started basketball and track in 5th grade. She has played volleyball, basketball and track for all four years at Seattle Lutheran (classified as 2B).
"A lot of the credit goes to my coaches," she said. "I have really good coaches. Every one of them is really supportive and wants to make me better. Throughout all the sports [at Seattle I've been really lucky."
Olivia has had the same coaches since her freshman year.
But to what does Olivia attribute her athletic success?
"I think a combination of being athletic and liking sports and having a good time," she said.
Olivia practices five to six days a week after school for two hours.
Olivia has also skated for two different junior roller derby teams, the Seattle Derby Brats (from 2007-'10) and the I-5 Rollergirls (2010-'14). She's skated ever since she was nine.
On top of school
But despite her heavy sports involvement, Olivia still maintains around a 3.5 GPA.
"I really like my life skills class," she said. "But probably English is my favorite subject."
Olivia said that next year she plans to attend university in Arizona.
"But right now I'm not planning on playing sports for the school," she said. "Just intermural teams."
Olivia Pride
Mark is an assistant track coach at Seattle Lutheran. He also served on the board of directors on both of Olivia's roller derby teams. He is currently vice president of the I-5 Rollergirls.
"She has the athleticism," Mark said. "I did sports growing up, but I never pushed her. She decided she wanted to do sports, and she's had fun doing it."
Olivia's track coach, Cynthia Lysen is proud of her student.
"I coached Olivia for four years," Lysen said. "She's the ultimate team player, putting the team before herself. She's always been willing to do that."
Lysen said this global opportunity is fitting for Olivia.
"She will be a great ambassador, not only for the game but for our country," she said. "It's especially good publicity given what the world thinks of American right now," she added. "I think sending kids over there to have fun and represent the country via sports is a wonderful way to go."
"There are vocal leaders and then there are leaders who lead by example," she said. "Olivia is someone who leads by example. She sets expectations and the other teammates see that. It's as important as having a vocal leader who keeps people in line by yells and screams."
Olivia has been a joy to coach.
"Here you have a person who comes with a head down, get to work, do the job but also understands why you're here. You can have fun and also be focused."
Lysen will miss Olivia.
"Olivia is the last generation of athletes I've had for nine years. I'll miss her a lot. I have stories, and they are all fun stories. I'm going to miss her leadership, miss her talent, miss her as a person. She's a wonderful, wonderful human being."
Olivia recently went to the state championships in track, taking first place in one of the competitions.
But while having the opportunity to participate in the United World Games is an honor, the tuition is steep. The program reportedly costs more than a couple thousand dollars.
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ForeSee partners with a variety of companies that complement our products and services, bringing our clients best-in-breed solutions that help achieve a 360° view of their customers across channels and touch points. Our partners include technology vendors, consultants, agencies, and other companies that complement our products and services.
Analytics & Digital Marketing Solutions | Agencies & Consulting | eCommerce Platforms | Service Providers & Technology | Tag Management
Analytics & Digital Marketing Solutions
Adobe® Analytics
Adobe® Analytics helps marketers tell the story of what's happening in their business with data by leveraging high-performance, real-time analytics across online and offline marketing channels. Marketers utilize the information that flows through Adobe Analytics to continuously improve the performance of their marketing activities. ForeSee CX Integrate for Adobe Analytics provides a simple to set-up, robust, and highly reliable integration between the ForeSee Analytics portal and the Adobe Digital Marketing Suite, allowing analysts to marry both attitudinal and behavioral data. The combination of these key metrics provides deep insight for analysts to combine robust travel pattern analysis with the sophistication of knowing site visitors' impressions, perspectives, and thoughts. In addition, the combined reporting option allows analysts to create insights on demand in the appropriate tool needed at that time.
Google Analytics
Google Analytics provides powerful digital analytics for anyone with a web presence, large or small. It's one of the most powerful digital analytics solutions available – and it's free for anyone to use.
IBM Enterprise Marketing Management (EMM)
The IBM Enterprise Marketing Management (EMM) Suite is an end-to-end, integrated set of capabilities designed exclusively for the needs of marketing organizations. Integrating and streamlining all aspects of online and offline marketing, IBM's EMM Suite empowers organizations and individuals to turn their passion for marketing into valuable customer relationships and more profitable, timely and measurable business outcomes. The IBM EMM Suite helps marketers understand customer wants and needs and leverage that understanding to engage buyers in highly relevant, interactive dialogs across digital, social and traditional marketing channels. Over 2,500 organizations around the world use IBM EMM solutions to help manage the pressures of increasing marketing complexity while delivering improved revenue and measurable results.
As the acknowledged global web analytics market leader, WebTrends enables thousands of web-smart organizations to easily improve campaign performance and web site conversion as well as to build stronger relationships with their customers. With WebTrends Marketing Lab, the company stands alone in offering comprehensive, customizable web analytics as well as real-time data exploration and customer targeting solutions for relationship marketing. Companies such as Microsoft, IKEA, Reuters, General Mills and more than half of the Fortune and Global 500 rely on WebTrends as their trusted standard for award-winning technology and a full range of consulting services, as well as unmatched industry expertise across the broadest range of vertical markets.
Agencies & Consulting
BitBang has provided digital intelligence and customer experience management consulting since 2003, offering a wide range of services enabling companies to create synergies between their online and offline worlds in order to improve overall business performance. By not bringing its own products and making its delivered value entirely dependent on the creativity and expertise it can bring to render the right analytical mix for every project, BitBang works for its customer's benefit.
Federal Consulting Group (FCG)
The Federal Consulting Group within the Department of the Interior assists federal agencies in improving their overall performance with a special focus on customer service. The group is made up of career federal executives who have extensive expertise in managing major programs and working with senior agency leaders. The Federal Consulting Group is the executive agent in the Federal Government for ForeSee and holds a generic clearance from the Office of Management and Budget for ForeSee surveys.
MVConsultoria is a leading Web Analytics and Customer Intelligence services firm with offices and clients in Spain, the United Kingdom and Argentina. The MVConsultoria team has been at the forefront of Web Analytics, data-powered automation and Customer Intelligence since 1998.
Salesforce.com is the enterprise cloud computing leader, helping companies connect with customers, partners, and employees in entirely new ways.
eCommerce Platforms
Ebay/GSI Commerce
Ebay/GSI Commerce is a leading provider of e-commerce solutions that enable retailers, branded manufacturers, entertainment companies and professional sports organizations to operate e-commerce businesses. The company provides solutions for its partners through its integrated e-commerce platform, which is comprised of three components: technology, logistics and customer care, and marketing services. The company provides e-commerce solutions for approximately 60 partners.
Oracle ATG Web Commerce
Oracle's ATG Web Commerce is the industry's top-ranked commerce solution that powers the world's best brands, and delivers a consistent, personalized cross-channel customer experience. ATG Web Commerce offers a complete commerce software platform that enables clients to deliver a personalized customer buying experience across all customer touchpoints, including the web, contact center, mobile devices, social media, physical stores and more. To further enhance the customer experience, Oracle offers an integrated set of software-as-a-service (SaaS) commerce services that provide the flexibility and intelligence to target the right visitors at the right time with the right offer in the right channel to drive high-value sales. Through these services, clients can engage with customers at the point of interaction through personalized recommendations, targeted promotions, and guided assistance.
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Branding Brand
Branding Brand powers the mobile commerce sites and apps for over 200 of the world's leading retailers, including American Eagle Outfitters, Costco, and Ralph Lauren. Founded in 2008 by three friends from Carnegie Mellon University, the company launched a mobile platform at the end of 2009 to seamlessly extend brands into optimized experiences for smartphones, tablets, and in-store. It is now the largest m-commerce provider to the Internet Retailer Top 500.
Rev.com is a new breed of service provider. Rev's proprietary technology platform is built to create a great work environment for translators and transcriptionists so the company can deliver clients ever increasing speed and quality. Rev's mission is to give more people the freedom to work from home, bringing the best of the office to their online workplace.
Providing customers excellent service — when they want it, with no wait time — is one of the most important factors in defining your brand. Verascape allows customers to help themselves with highly professional, easy to use voice automated services. With real-time links to your database, Verascape can provide up-to-the-minute customer service at a fraction of the cost of a live agent.
Usablenet is a global technology leader for mobile and multi-channel customer engagement. As the pioneer in usability and web accessibility, Usablenet's powerful platform enables leading companies across all industries to create compelling experiences for their customers across multiple channels, including mobile, tablet, social, and in-store kiosks.
Tag Management
BrightTag is one of the world's leading marketing technology platforms, powering more than 1,000 brands and agencies. BrightTag empowers its clients to collect, connect and act on cross-channel customer data in real time, from websites and beyond – including mobile, social, email, ads, point-of-sale, CRM, call center, kiosks and more. This enables brand marketers and agencies to deliver smarter, more relevant omni-channel marketing that improves engagement, conversion and loyalty.
Ensighten offers the industry's first Real-Time™ Tag Management System built for the needs of sophisticated enterprise websites. An intuitive interface empowers digital marketers to easily add, remove, or modify any third-party digital marketing tag or pixel in real-time, without IT or agency dependency. Ensighten Manage is the only Tag Management System that enables precise tag control per visitor, per session, per page, and works seamlessly across all platforms and devices: web, apps, tablet, phone, e-reader, kiosk, game console, and more.
TagMan is a leading global Tag Management System, Marketing Data, and Marketing Attribution provider. Since 2007, e-commerce professionals and digital marketers have relied on TagMan to improve website performance and harness third party vendor tags. TagMan's industry first Marketing Data Platform (MDP) provides access to TagMan's marketing data layer collected in real-time and unified by tags. Marketers can now visualize the customer journey and path to purchase through comprehensive dashboards to reveal the true impact of advertising spend and drive revenue through tag-powered data.
Founded in 2008, Tealium is the leader in enterprise tag management and digital data distribution platforms. Tealium gives marketers a comprehensive solution for managing their third-party digital marketing vendor tags, and then correlating the data those tags generate into a clean, actionable source. Hundreds of major brands on five different continents rely on Tealium to streamline digital marketing complexity, solve their customer data integration challenges, and improve the effectiveness of their customer acquisition and retention campaigns.
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Contact us to learn more about partnering with ForeSee, the leader in multichannel customer experience analytics, or read more about our data integration capabilities.


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The U.S. Federal Communications Commission is prepared to impose new regulations on network providers if they don't take steps to improve cyber security, according to PCWorld. Speaking at the American Enterprise Institute for Public Policy Research, FCC Chair Tom Wheeler said that the agency will work to promote, rather than regulate, initiatives meant to improve security. But if those efforts on the part of companies like AT&T (NYSE:T), Verizon (NYSE:VZ), Sprint (NYSE:S), and Comcast (NASDAQ:CMCSA) don't succeed, then Wheeler says that the FCC will step in.
We believe there is a new regulatory paradigm where the commission relies on industry and the market first while preserving other options if that approach is unsuccessful.
That "new regulatory paradigm" sees the FCC asking network providers to voluntarily adopt cyber security practices aimed to protect against malicious attacks by hackers. According to The Washington Post, the FCC also wants to bring companies together to work on technologies to prevent cyber attacks and protect users' privacy. Wheeler said that the FCC hopes to develop "market accountability that doesn't currently exist," holding communications responsible for protecting infrastructure and customer data system. The goal is to prevent, or at least identify and respond to, breaches like the massive ones that exposed customer information at Target (NYSE:TGT) and Neiman Marcus.
The new initiative follows the March introduction of cyber security recommendations by the National Institute of Standards and Technology. The FCC will also evaluate whether network providers have implemented the 2011 recommendations made by the Communications, Security, Reliability, and Interoperability Council, an advisory committee to the FCC. The FCC refers to cyber security as a matter of public safety, and wants companies to report threats not only to the FCC, but to each other.


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Hello. I am a 26 year old female at my ideal weight. However I feel I have small jowl-like bulges of fat on my lower face which I dislike. Would buccal fat removal give me a more chiselled look? As well as buccal fat removal,various doctors have also suggested facial liposuction, jaw muscle reduction via botox or surgery and even jaw bone shaving. I'm extremely confused now and don't even know exactly what it is that is 'wrong' with my face anymore. Please help!
Am I the Right Candidate for Buccal Fat Removal?
Doctor Answers 6
Candidate for Buccal Fat Removal
Hello London. It is difficult to determine from your photo if you are a candidate for buccal fat pad removal. The best view to determine this is a frontal one. You can simulate the effect of removing your fat pads by pursing your lips and lightly suctioning your cheeks. Buccal fat pad removal results in a more triangular lower face. I would not recommend liposuction of the face. Botox injections to the masseter muscle also helps to decrease the roundness of the face. From El Paso.
Choosing a candiate for buccal fat removal
Patients must be selected properly for buccal fat pad removal, as chosing an inappropriate candidate can lead to a premature gaunt look in the face.
Critera to see if candidate for buccal fat pad removal:
1. Family history of "full" faces- Families with full faces who age with full faces are likely to benefit from buccal fat removal. Families whose fuller faces eventually turn gaunt should avoid this procedure
2. Buccal fat excess- There are multiple fat pads in the face. The key is to determine if buccal fat pad removal is beneficial
Web reference: http://www.shahfacialplastics.com/buccal-fat-removal.html
Buccal Fat Removal for a 26 y.o. Asian female
Dear London9490,
With the photograph you have shared, it is hard to give you a definitive answer to whether Buccal fat pad removal is the best procedure for you to achieve the ideal midface contour or not.
Nonetheless, you may be a good candidate for this procedure performed conservatively and supplemented with Botox injection and additional facial sculpting procedures.
Definitely, I encourage you to seek a consultation with an extensively experienced surgeon who will guide you to make a well informed decision.
Best of luck to you.
Dr. Sajjadian
Web reference: http://www.DrSajjadian.com
You might also like...
Am I the Right Candidate for Buccal Fat Removal?
First you must be sure if these are the real problems before deciding on surgical correction. Some Asian patients have excessive buccal fat pad which can be easily removed and therefore get a more triangular face. The other procedures are also done particularly in patients who have prominant angle of jaw, which is in fact desirable in Caucasian patients who request jaw implants to enhance these.
Buccal Fat removal is not to be taken lightly
If "you don't even know what is wrong with your face anymore", maybe any permanent plastic surgery is a bad idea. You really need to be confident and certain that you are doing the right thing for the right reason and that you will predictably be happy. If there is any doubt, don't do anything permanent. Sticking to Botox for Masseter muscles is temporary, but I see why you are confused. In my view, masseter injection makes you look less sculpted, not more chiseled. Building on your already good cheek bones and add to your angularity, and if a temporary filler is used, you don't have to worry about regretting the decision. My point is, you must be really sure about it before having surgery.
Buccal fat pad reduction
Reducing the buccal fat pad usually is done for the effect of creating a more chiseled appearance of the cheek and cheek bone. In your case it seems that you already have fairly good definition there. I would probably not recommend that procedure for you. In my practice I reserve buccal fat pad reduction for patients with a really full cheek.
I hope this info helps!
Web reference: http://www.dr-rubinstein.com/cheek-reduction.html
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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Thanks for keeping us all up to date Donna..:))I'm digging thru closets here for a garage sale and to rid of last year kid stuff and make room for the new;))Recycle is the game!:))So, defin. gives me more time...thanks Skeat
Welcome to the Quilting Board!
Thanks for keeping us all up to date Donna..:))I'm digging thru closets here for a garage sale and to rid of last year kid stuff and make room for the new;))Recycle is the game!:))So, defin. gives me more time...thanks Skeat
Okay, I just sent you Tina's last email a couple of days ago. But she just sent this one. Apparently while she is gone for vacation (wedding) she is haveing a sale. Anyway here is the email.
Just a quick note to let you know that I'll be having a "Vacation Sale" while I'm away on well.....vacation!! It will run from today, August 5th through Sunday, August 10th. I'll be shipping orders out on Monday the 11th.
What a great time to stock up for those Christmas projects that are just around the corner.
My sincere apologies to those waiting for the next mystery quilt project. Life just plain got in the way so the next one won't be posted until after I get back from my trip.
I hope you have a wonderful, wonderful week!
Happy Quilting and God Bless,
Everybody...wake up!!! She has posted the new mystery tote!! Donna...I couldn't find the tote thread...you can redirect this in the right spot if you want...everyone go to: http://rockymountainquiltworks.store...terns/list.htm Let's get mov'n!!! Great day to play here too!:))Skeat
Well dang it she must have done it last night! I sent her an email. I guess you did not see the other thread but thanks for letting us know. I will have to get the other thread for you. Should be near top.
Hello everyone!
I'm so glad you're enjoying my mysteries. You all did awesome jobs on your tablerunners!!! Wow! I'd love to be able to post some of your beautiful creations on my website. I'm constantly amazed at the talent that is out there and love to see how other people put fabrics/patterns and colors together. It is truly an inspiration to me.If you have any questions or problems with any of the Mystery patterns, just give me a holler and I'll be more than happy to help. When I do my patterns, I do a step, take a picture, do another step, take another picture lol....so I'm making one right along with you! I'm doing my tote bag with the focus print of the blueberries and will be giving it to my Mom for Christmas(never too early to start!). I also belong to another online quilt group and am working on swap blocks for that this afternoon, so I had better get back to my machine. I hope everyone has a fabulous day!!
Tina, if you go to the addy below, that is the page we are doing the tote on. You will see a lot more people doing it and I posted your email note you put out this morning. Anyway if you want to stop in go to that page (MYSTERY TOTE) so we know you have been here. LOL. I am going to send everyone here to see your message,.


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The North Face Resolve Jacket - Women's - $89.95
Fend off torrential downpours thanks to The North Face Resolve Jacket. Waterproof and breathable HyVent 2L shell fabric allows sweaty vapor to escape from within, and shrugs off heinous weather on the outside. At less than 17 ounces this jacket packs easily without weighing you down on long treks. An interior mesh lining provides a buffer between your under layers and the outer fabric, and a hem cinch cord battens down the waist. Flip up the adjustable hood when the skies turn particularly nasty, but don't worry; fully-taped seams add an additional measure of weatherproofing so you won't spring any unexpected leaks.\012\012 The North Face used a fully adjustable hood to round out the Resolve Rain Jacket's features and ensure you stay bone-dry even in nasty weather. - $89.95


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Minnesota Sheriffs' Association scholarship recipients announced
WALKER — Cass County Sheriff Tom Burch announced this week the winners of the Minnesota Sheriffs' Association scholarship program for 2011. The association awards up to 15 scholarships each year to individuals planning to become Minnesota peace officers. The applicants apply through their local sheriff's office. This year the scholarship committee chose 15 winners out of the broad spectrum of applicants. The Minnesota Sheriffs' Association scholarship program, which is promoted by the 87 sheriffs of Minnesota, is pleased to announce the winners. They are:
Kodi Nelson, Roseau; Adam Cook, Aitkin; Seth Robison, Longville; Tony Brill, Little Falls; Jacob Letourneau, Winona; Joshua Keller, Montevideo; Columba Nwosu, Willmar; Angela Otto, Belle Plaine; Brendan Mooney, St. Cloud; Jacob Wrobbel, Montrose; Shawn Bohnen, Sleepy Eye; Brady Stough, Redwood Falls; Jared Warneke, Zumbrota; Troy Fitting, Houston; Rebecca Dick, Reads Landing.
The members of the Minnesota Sheriffs' Association offer their congratulations to each of the scholarship winners. The qualifications and academic excellence shown by these winners reflect the dedication and pride of the young people in the state of Minnesota who will enter the difficult but rewarding profession of law enforcement and public service.
The awarding of the scholarship to the individual winners will take place at a later date and will be made by the sheriff in the winner's home community.


text: Hi all. I did a search of the forum and did not see anything which matched so if this has been answered before, I apologize. I completed the ISPConfig install without error on my Debian Sarge box. When I go to the admin site and try to login with admin/admin, I get these error: Warning: mysql_connect() [function.mysql-connect]: Access denied for user: '[email protected] Warning: mysql_connect() [function.mysql-connect]: Access denied for user: '[email protected] Warning: Cannot modify header information - headers already sent by (output started at /home/admispconfig/ispconfig/lib/classes/ispconfig_db_mysql.lib.php:76) in /home/admispconfig/ispconfig/web/login/login.php on line 56 So, I head over to the ispconfig_db_mysql.lib.php file and noticed that the information for host/user/password are all blank. I populate those fields and now I get this error: Parse error: parse error, unexpected '\"' in /home/admispconfig/ispconfig/lib/classes/ispconfig_db_mysql.lib.php on line 36 Line 36 is the password line which does not have any '\' entries. I'm not sure where to go from here. Any pointers or some text reference would be greatly appreciated. Thanks in advance!


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Indiana deputy rescues couple from smoke-filled home
Authorities say a southern Indiana sheriff's deputy rescued an elderly couple from their smoke-filled home by kicking in its front door and awakening them.
The Herald-Times reports Monroe County Sheriff's Deputy Allen Mullis saw smoke billowing from the couple's garage and house about 3 a.m. Thursday as he patrolled their neighborhood.
He kicked in the home's front door and led 80-year-old Edward Arnold and 83-year-old Mary Arnold to his patrol car for safety. Perry-Clear Creek fire Chief Joey McWhorter Jr. says it's lucky the deputy happened by the home when he did because the sleeping couple hadn't realized there was a fire in their garage.
Firefighters determined that either the cord to an electric leaf blower or a faulty wiring connection inside the leaf blower sparked the fire.
(Copyright 2013 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)


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In the last 90 days, 9 listings have been posted online, which means listings are probably leasing quickly. You'll want to be prepared to tour spaces as quick as possible. There aren't many listings below 2,000 square feet on the market, so finding a space for a small business might be tough.
Even though the median size is 3,250 sqft, there are still 0 leases below 2,000 sqft. Most properties in this area are around 2 floors. Most buildings in this area were built in 1992 and are Class A.
Not much of the real estate is used for commercial space, so it might be hard to find office space. Utilities is the dominant industry in this area. Commute by walking is the most popular way to get to work amongst South Buffalo occupants. Commuting under 5 minutes a day is the norm for those who opt to reside in this part of town.
Trends and status of the commercial real estate market in South Buffalo.
Market Report
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Pack less. Be more.
@lyrad1Locale: Pacific Northwest, USA, Earth
Check this out:
Could this revolutionized medicine?
I think Doug posted something similar a while back where one could use the smart phone to predict altitude sickness problems.
@francoLocale: Tarptent
great stuff , still I don't own a mobile phone.
BTW, a liberal translation ,mon Dieu, of Part Dieux could be :Semi Gods
was that your intention ?
@lyrad1Locale: Pacific Northwest, USA, Earth
Thanks for the translation.
No, "semi gods" wasn't my intent. I was trying to copy the title of a funny movie I saw and thought the word was French for "two".
I must learn to not use words I don't understand. It could get me into trouble some day.
@francoLocale: Tarptent
Sorry, I was just having some fun with it because the English expression "part deux" is in itself a sort of play on words.
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Silicon Beach Training offer a broad range of courses within IT, business and management. For an interactive, hands-on approach to training contact us today. We offer training in London, Brighton and all over the UK.
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Life insurance provides a death benefit for an individual's beneficiaries. It
allows survivors to pay off debts and other expenses; it can also provide a
source of income to replace that lost by the death of the insured. There are two
primary types of life insurance: term, which provides insurance for a specified
period of time at a lower cost; and permanent, which provides a certain amount
of coverage at variable rates. There are other variations, including universal
life, which builds cash value and is widely marketed by life insurance
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Donor-Advised Funds provide many options and opportunities for donors. Establishing your own Donor-Advised Fund can be as easy as signing an agreement and either writing a check or making a gift of some other asset you own.
When you create a Donor-Advised Fund at the Foundation, you can:
Add gifts to the Fund whenever, and as often, as you wish;
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Decide later what charities you want to recommend to receive distributions, along with how much and when;
Pass along the important values inherent in giving by involving children and other family members in the distribution recommendations.
Donor-Advised Funds are perfect for donors who wish to take advantage of current tax laws regarding charitable gifts, but are as yet unsure as to how, to whom, and in what amounts, they want to direct those gifts. For more information about the advantages of a Donor-Advised Fund, please contact the Foundation.


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Michele Fife is the WOTV 4 Women wellness expert focusing on natural health and Yoga. She is an Ayurvedic Yoga Therapist and Nutritionist and sees clients at her downtown GR location as well as teaching public classes at Expressions Of Grace and Mary Free Bed Hospital.
"I love being able to participate in the process of positive change! It has been such a gift to me over the years to actually watch and get to know so many bright souls as they embark upon a wellness journey."
She lives a Yogic lifestyle with her husband Jon, 2 cats and 7 chickens. In addition to practicing yoga you might find Michele enjoying nature, cooking and entertaining, or exploring the world. Her love of learning never stops and as a WOTV 4 Women expert she hopes to share information and tips based on her own life experiences, trials and successes.
Contact Michele: Customized health & Wellness services


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Florida ID: 6520
1285 Gator Trail
This home has been sold
We have 651 similar homes for sale.
- Price$339,000
- TypeHouse
- Bedrooms2
- Bathrooms2
- Garage Spaces2
- Approx. Sq. Ft.2,447
Key Features
- Deck
- Pool
Bear Island, West Palm Beach, Florida - Completely renovated 2 bedroom 2 bath corner lot house with pool and a 2 car garage offers lake and golf views. Completely renovated 2 bedroom 2 bath corner lot house with pool and a 2 car garage offers lake and golf views. This unique property offers an open floor plan, large imported tile, new HVAC including new duct work and all 6 ft doors have been replaced with new 8 ft doors. The open kitchen offers imported cabinetry, marble counters, stainless appliances including a wine cooler and an eat-in kitchen bar. Both bathrooms offer imported Italian tile, Toto toilets and designer fixtures. Outside areas are large with lake views from the pool and spa. Convenient to downtown West Palm Beach, the airport and minutes to the beach.
Agent Ask me a question
email@example.com Email Me See my 9 other sale listings
West Palm Beach
The City of West Palm Beach, with its sunny palm-lined streets, historic neighborhoods, and year-round outdoor festivals, is a vibrant destination that continues to increase in appeal.
Future plans call for the continued development of the downtown waterfront district. Today, an increasing number of year-round residents mix with the seasonal visitors to enjoy the fashionable lounges, cozy pubs, eclectic boutiques and fine restaurants that stretch from downtown's CityPlace to Clematis Street. Art and antiques collectors from around the globe come to West Palm Beach in search of one-of-a-kind pieces available at the dozens of stores lining "Antiques Row" on West Palm's Dixie Highway.


text: Plot: Just prior to World War One the hard drinking sharpshooting, Irish American Colonel Flynn O'Flynn, uses British aristocrat Sebastian Oldsmith to help poach ivory from German controlled territory in East Africa, putting them at odds with Herman Fleischer, the local German Provincial Commander. When Sebastian is infected with malaria he is nursed back to health by Flynn's daughter Rosa, they fall in love and marry. Not long after Britain declares war on Germany and they are drawn into the conflict, ultimately making a daring attack on the German armored cruiser SMS Blücher as it undergoes repairs in a local estuary. Genre: Action | Adventure | Drama | Thriller | War IMDB Rating: 6.3/10 from 1,650 users Directed by: Peter R. Hunt (as Peter Hunt) Starring: Lee Marvin, Roger Moore, Barbara Parkins Release Name: Shout.at.the.Devil.1976.720p.BluRay.x264-TRiPS Size: 5.46 GB Video: MKV | 1280 × 720 | 4610 Kbps Audio: English | AC3 | 640 Kbps Runtime: 149 mins Information: Code: http://www.imdb.com/title/tt0075214/ Downloads: Code: Download from REDBUNKER http://www.redbunker.net/nq5q3do7okcc/Shout.at.the.Devil.1976.720p.BluRay.x264-TRiPS.mkv.html Enjoy!


text:
MMA Forum - UFC Forums - UFC Results - MMA Videos (http://www.mmaforum.com/forumindex.php)
- Bellator FC (http://www.mmaforum.com/bellator-fc/)
- - Bellator FC 39 (http://www.mmaforum.com/bellator-fc/89491-bellator-fc-39-a.html)
Bellator FC 39
Bellator 39
Date: Apr 02, 2011
Location: Uncasville, Connecticut
Venue: Mohegan Sun
Broadcast: MTV2
Alvarez should definitely take this fight. Hopefully Ben Saunders gets a win here as well.
I'm pulling for Killa B as well, hopefully he can work his way back into the UFC but they have good welterweight wrestlers in other companies too. I was bummed to see they pushed back that Hieron vs Weedman fight back, but they moved up a good replacemtent in Imada/Freire if that really goes down this weekend. Here are some pretty good under card fights for Bellator:
So we see Eddie's first title defense. The way he has been walking all over people I think he should win this. But then again this is MMA.
Really good card, im looking forward to the middle 2 fights most, think their both really close. I expect Alvarez to beat Curran comfortably, 2nd round TKO i think, and the same with Saunders. Good v Hawn is very close, Hawns biggest challenge by far but i think Good's experience against the better oposition will be the difference and he'll win by decision. Imada v Freire is a really good style match too, i think Freire will look good early but Imada will get it to the mat eventually for a 2nd/3rd round submission.
Alvarez, Good, Imada and Saunders for me, should be some fun fights though. Veach v Nazare should be a really good fight too, 6-0 prospect v UFC vet, I think Veach wil ltake it though he's a solid fighter. Looking forward to it.
Looking forward to seeing Alvarez again.
I really want him in the UFC. :(
This is a good card; gonna be some good fights. Alvarez/Curran will be close I think.
Lee's face looks like he was a murder victim in a horror movie.
That was grotesque...brutal, brutal fight.
How do you think Alveraz's match will be close?
pitbull's knee was brutal...best ive seen in a long time
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PDC Darts World Championship - Darts Blog 5
Posted: 21.12.10 in Tournament Analysis Blog category
We admire a `well oiled` James Wade and suggest a look at a German text book.
Scouting for Professionals
Be Prepared! This is the motto for the Scout movement and also for professional darts players. We touched on the benefits of being properly prepared for a darts match and speculated that if James Wade wasn`t 100% prepared for Antonio Alcinas then there could be a shock on the cards. As it was "The Machine" was well oiled (in a good way) and left his "silly head" behind as Rod amusingly described the head belonging to James that ended up an alarming shade of red against Scott Waites recently. Rod was of course referring to Wade`s inclination to perhaps not consider the potential consequences of his pre-match routine. As it was James played brilliantly and left "Toni" looking ordinary.
Darts Miscellany
Thanks to Mike from Sheffield who came out of the hat to win a copy of the Darts Miscellany. Mike`s list was in celebration of the sponsorship by Ladbrokes and here is his collection of bookmaker`s phrases.
11/8 Up the Arm
3/1 Carpet
100/30 Burlington Bertie
10/1 Cockle
33/1 Double Carpet
2/1 Bottle
100/1 Century
Aim and Fire
There were some brilliant analytical points picked up by the Sky team last night in areas that all standards of players can benefit from. Sid picked up on "The Prince of Wales" hitting the big number and then in a split second he had hit the double 12, leg over! This `rhythm` as opposed to momentum is a valuable weapon for a darts player. The key to it is to know in advance exactly where your final destination is and being a skilful counter like Burnett or Whitlock is vital.
From a psychological viewpoint this method does have its benefits, there is no time for the anxiety to grow, there is no time to consider how vital the shot is and thus increasing the stress and for Richie Burnett this `natural` way of playing worked for him.
However, tonight we will most likely see the other side of the coin. Steve Brown advocates an approach we are also fans of at the Darts Performance Centre. He will stop when there is an important double to be hit. His method is to attempt to completely relax and then focus solely on his objective of hitting the double, then he throws.
Then to add further fuel to the "what is the best approach` debate we have Jelle Klassen. He hasn`t just got his foot but his whole body in the "Burnett camp" when it comes to the contemplation of the shot routine! We will probably be all left pondering once again tonight at some point that if only Jelle slowed down a fraction when it came to hitting the double he may be a more effective player. But that`s how he plays.
So what is the best approach? Well first of all we would recommend getting your counting sorted out and not to throw a dart until you know exactly where you are heading. After that it is down to the style of the individual player and also whether they possess the skill of being able to shut out stress and anxiety, if you can at least you have a choice of which style to adopt.
A German Text Book
John Gwynne admired the German Jyhan Artut last night for his focus, we agree, despite the fact he lost. His throwing technique may not be `text book` but it`s not far off, He is, however, like a statue when he throws. His eyes did not move from the target until the third dart hit the board. Also, there was not any "unnecessary" shuffling up and down the oche by the German, he left his skill and his darts to find the correct destination, now that is text book!
Author: Paul Gillings ( firstname.lastname@example.com )
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text:
Entries are now open for the prestigious Prime Minister's Science Prizes, and a former winner says New Zealand scientists need to put aside fears of Tall Poppy Syndrome and take a shot at the $1 million on offer.
The prizes, launched five years ago, aim to highlight how science underpins innovation and helps build a more competitive and productive economy.
Andrew Coy was part of a team of Victoria University researchers led by the late Sir Paul Callaghan who won the most valuable prize - $500,000 - for their work researching magnetic resonance. Their discoveries are widely used in medical magnetic resonance imaging (MRI) and materials science, and the technology they have developed is earning millions of dollars.
Dr Coy, who is the chief executive of technology company Magritek where he and the team are continuing their work, said the majority of the prize money was given to Victoria University to set up a PhD scholarship in magnetic resonance. A small amount was kept by the team, but by far the greatest benefit of the prize was what it meant for their reputation.
"Shortly after we got that award we went to a very big conference in China and it was just great to be able to have pictures of our team standing there with the Prime Minister of New Zealand. In some countries that can be a very powerful thing. From a reputation and company PR point of view it was unbeatable, just fantastic."
The company had continued to develop and grow its revenue, in no small way due to the prize.
"You win a national science prize and people do take notice and take a second look."
Dr Coy had one piece of advice for scientists thinking of applying for the prizes: "Sometimes in New Zealand we think it's bragging or a bit egotistical to talk very positively about what you're doing, but it's absolutely essential if you want people to know about what's happening in your area of science. Get out there and tell the story about how it has an impact on society and how it's going to have a positive impact for New Zealand," he said.
"The ironic thing is in New Zealand we're incredibly good at it - we do all kinds of innovative, creative, really fantastic things when you compare them to the rest of the world. But for some reason we don't jump up and down and celebrate them as much as we could."
Entries can be lodged up until July 17.
The prizes:
- The Prime Minister's Science Prize, $500,000
To an individual or team which has made a transformative discovery or achievement in science that has had a significant impact on New Zealand or internationally
- The Prime Minister's MacDiarmid Emerging Scientist Prize, $200,000
To an outstanding emerging scientist undertaking research for a PhD or within five years of the date of the award of a PhD
- The Prime Minister's Science Teacher Prize, $150,000
To a science teacher for outstanding achievement in teaching science
- The Prime Minister's Science Media Communication Prize, $100,000
To a practising scientist who is an effective communicator, to provide them with an opportunity to further develop their knowledge and capability in science media communication
- The Prime Minister's Future Scientist Prize, $50,000
To a secondary school student for outstanding achievement in carrying out a practical and innovative research or technology project.


text:
August 17, 2012 at 05:31:09 Aug 17, 2012 @ 05:31
that was exhilarating. Picked up the All black paisley and the all black w/ white stars and stripes piff
add me on steam: LITTERLYFE ////// CS: GO //// instagram: @alecdeltaco tumblr: alecsayss510.tumblr.com
i'll be at work still at the time of the drop, would you consider this "limited"?
Releasing tonight at 12am EST
that was exhilarating. Picked up the All black paisley and the all black w/ white stars and stripes piff
mister on point as usual! picked up a couple tees myself
Black Independence copped!
If the blue paisley is still up by Saturday I'll cop it too gotta get my cuhz on hahaha
Mister is killin the game right now
got my black paisley..
ig @hen7ai https://www.grailed.com/anoma1y
info on sizing. tts ?
Originally posted by Inactive UserOH HELL YES. WAS STOKED FOR THAT BLACK/WHITE INDEPENDENCE, BUT Y'ALL MADE A BLACK/BLACK? SURPRISE SURPRISE
is this the black/white paisley? pic is black/black paisley
copped the blk/wht independence, blk/wht paisley, mr.clips in natural, and mr.keys in natural. hook me up with some stickers if you guys have some! THANKS fam
FS: Supreme SB's /Black Scale/Diamond/5TH column M/L DS http://hypebeast.com/forums/apparel/172320/
considered copped! You need to tell gem to throw some sample goods down when he comes to socal lol im cash ready
Will do pimp. Gem is the homie!
What kind of blanks are the independence/paisley tees made on?
Will do pimp. Gem is the homie!
btw @MrSFC what does it mean when it says "awaiting fulfillment" did my payment go through?? my order #'s: 3423 and 3411 thanks!
Black o' black Independence — Beautiful.
Shouts @ Jereme, Joelle & Tom.


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Unique apartment 200m from Vondelpark
Maks. liczba gości4
Rozmiar60 m²
- Przyjazny dla rodziny/dzieci
- Internet
- Kuchnia
- Zmywarka
- Łóżeczko dziecięce
- Ręczniki
- Balkon/taras
Podsumowanie obiektu
|Legmeerstraat, 1058 Amsterdam, Niderlandy
|Rodzaj nieruchomości
|Kod referencyjny
|Wskaźnik odpowiedzi
|Czas reakcji
|Kilka godzin
|Zaktualizowano kalendarz
This apartment is located on the ground floor making it also very easy access to "young" families and the elderly. The apartment is +/-60 m 2 in size and has 2 bedrooms, living room, kitchen, separate bathroom and toilet a unique 60 m 2 large garden with lounge sofas.
This comfortable apartment is trendy decorated, lots of light and very quiet environment, making even a good night's rest is assured in the middle of the city!
Within 5 minutes you are on one of the upcoming streets of Amsterdam, named the Amstelveenseweg with many restaurants and bars. But also the special Vondelpark with all its "relax" spots. The museum district with the Rijks, Stedelijk and van Gogh museum within 20 minutes ' walk, 10 minutes by bike or within 15 minutes by public transport.
In short a great place from which you can easily discover Amsterdam and where you can feel your Amsterdammer under the people of Amsterdam!
RegulaminSmoking is not allowed in the apartment. No pets There is a manual provided in the apartment with all House rules
|Cena za >3 gości
|176 zł opłata dodatkowa od gościa/nocy
|Zameldowanie po
|Zameldowanie przed
|Do ustalenia
|Wymeldowanie przed
|Zasady Anulowania Rezerwacji
Beautiful and wonderfully decorated apartment in a prime location.
Very nice and friendly hosts and a good communication.
Due to illness we couldn't leave to the appointed time, but it was no problem to stay a few hours longer, so we could go directly to the airport.
Thanks again Brigitte.
Very quiet an nice area, close to the beautiful park that will take you downtown in a nice 20 minute walk. Nice garden, all clean, good communication. Perfect.
Brigitte's apartment was absolutely fantastic. Lots of space and a lovely place to return to at the end of a busy day. Especially good for families - a garden for playing, a park at the end of the road, and the Vondelpark a few minutes away. Plus shops/bars/restaurants around the corner. We definitely hope to be back!
Very nice and clean apartment. Quiet neibourhood and only a few minutes from tram and bus.
Awesome apartment! We realy enjoyed our time and we would like to recommend flat & booking to everyone else. Thanks a bunch!!!
Excellent location. A lovely, well equipped walkout. Easy access to town centre. Excellent communication with the host, Brigitte.
we had a wonderful stay in Amsterdam. Good correspondence with Brigitte. Apartment was beautiful, nice and quiet and had everything we needed. Ideal location and easy to get to by tram. Would definitely stay here again.
Great apartment, great contact. Brigitte provided everything one could hope for. Well furnished, everything in the advert was there, no surprises. We really enjoy our time in the apartement and of course in great garden. We stay with our 1 1/2 jear old daughter and she loved it to ran around and play in the garden. Never stayed in the area before, but its a great place with supermarkets, coffee shops, restaurants and playgrounds, just 20min away from the rijksmuseum.
Probably the best apartment ever stayed at in Amsterdam, highly recommended!
- Gwarancja jakości zakwaterowania
- Każdy gospodarz jest weryfikowany telefonicznie
- Bezpieczna płatność za pomocą szyfrowania SSL


text:
Halo Fest Coming to PAX Prime 2011
Posted on Tuesday, May 3 @ 15:22:47 PST by Blake_Morse
It's time for PAX Prime 2011 Attendees to brush off their cardboard Master Chief suits and get ready to party down. To celebrate 10 years of sticky grenades and tea bagging there's going to be a huge Halo party in Seattle, WA. during Penny Arcades annual expo. So far, all we know is that your PAX badge will get you in, but after attending a few Microsoft sponsored bashes myself, I can guarantee that this is going to be one hell of a bash:
That last line makes it sound like we'll getting some sort of announcement at the event as well. Could it be the long rumored Halo Kinect game? Or perhaps it will be something about the supposed re-launch of the series. Either way I'm extremely happy that I'll be going to Seattle in September to find out for myself. More news should be coming via the Halo: Waypoint soon, so we'll keep you posted as we find out more.
|More On GameRevolution


text:
26th April 2012: In his fourth and final AFCO contribution of the morning, Andrew Brons spoke during a debate on how member states might be encouraged to implement directives* into national laws.
(The Rapporteur had suggested than an answer to the 'problem' of member states not implementing directives would be to put more EU laws in the form of regulations which are self-enacting and come into force automatically in member states without having to be implemented.)
"I shall speak only briefly but I would not want my silence to be interpreted as acquiescence. I favour directives over regulations because they (directives) can be disobeyed by member states - not legally but in fact. I say that the more disobedience by member states the better!"


text:
The Hand of Ethelberta HTML version
2.Christopher's House - Sandbourne Town -
Sandbourne Moor
During the wet autumn of the same year, the postman
passed one morning as usual into a plain street that ran
through the less fashionable portion of Sandbourne, a
modern coast town and watering- place not many miles from
the ancient Anglebury. He knocked at the door of a flat-faced
brick house, and it was opened by a slight, thoughtful young
man, with his hat on, just then coming out. The postman put
into his hands a book packet, addressed, 'Christopher Julian,
Christopher took the package upstairs, opened it with
curiosity, and discovered within a green volume of poems,
by an anonymous writer, the title-page bearing the
inscription, 'Metres by E.' The book was new, though it was
cut, and it appeared to have been looked into. The young
man, after turning it over and wondering where it came from,
laid it on the table and went his way, being in haste to fulfil
his engagements for the day.
In the evening, on returning home from his occupations, he
sat himself down cosily to read the newly-arrived volume.
The winds of this uncertain season were snarling in the
chimneys, and drops of rain spat themselves into the fire,
revealing plainly that the young man's room was not far
enough from the top of the house to admit of a twist in the
flue, and revealing darkly a little more, if that social rule-of-
three inverse, the higher in lodgings the lower in pocket,
were applicable here. However, the aspect of the room,
though homely, was cheerful, a somewhat contradictory
group of furniture suggesting that the collection consisted of
waifs and strays from a former home, the grimy faces of the
old articles exercising a curious and subduing effect on the


text:
You know how I said that I wouldn't wear yesterday's Couture Dress? That goes double for this one. Yikes!
Let's just say… this one is special.
I'm pretty sure I'd try to put this on and get lost in it. I'm confused just looking at it! Here is how it was described in the etsy post:
This is a one-of-a-kind made with several pairs of sliced up denim jeans and multiple pockets galore. This is a couture piece and was built on a live model. Fits 32-35 bust and 34-38 hip. Must be at least 5'2 or the bottom will hit the ground.
Yeah… just yeah. I bet some of you could rock this, right?


text:
Kattreya Model
Eleanor Marie PhotoPhotographer Female London, England, United Kingdom
My Website: http://eleanormariephotography.tumblr.comMy MM URL: http://www.modelmayhem.com/kaikee
Mayhem # 2737041
About MeHello and thank you for taking a look at my page. I am here as a beginner photographer who is eager to learn and mix with other creative people.
I have worked as a model for a few years now - http://www.modelmayhem.com/1641825 - but am looking to move more into photography at this stage. I have a real desire to grow and learn, and feel inspired by those photographers that I have modelled for in the past.
Please get in touch if you would like us to work together - although I don't yet have a vast amount of experience, I hope that having been a model for so long will enable me to create a comfortable and enjoyable shoot experience for you.
Eleanor Marie
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text:
Ok, Ok, I have read threads about how, after getting the WRX, you get used to the power very quickly and it can seem slower after a while. That has happened to me...
I bought the WRX coming off a Civic HX, took it easy through the first 1K (welll, uh....i got on it a FEW times actually). Now it has 3K and over the last 500-1000 miles I don't feel that "punch" that I felt when I first got on it. The first time I was like "hell yeah...this thing PUNCHES on boost" now I'm like...."where's the power at"? Not that it feels slow, but just less punch.
Yeah I live in FL and it has gotten warmer...don't think that makes a big diff.
The new stereo won't help with added weight/sub box.
What are the realistic chances that something is wrong in the turbo/powertrain?
Perhaps I am just one of the people that will soon be in a 12-step program for addiction to mods?
Perhaps it is just the "butt dyno" getting used to things after the Civic, and I am crazy, need to think of my "happy place" and be held for a while?
This happen to a lot of people? (BTW I love the car, it's quick, and I get compliments/questions from random people at least once a week about it, unfortunately it hasn't been young redheads going "wow, nice blue color...can i sit in your lap while we cruise?")