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The Elephantantrum
Written by Gillian Shields
Illustrated by Cally Johnson-Issacs
Lovereading4kids Price £9.59
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The Elephantantrum by Gillian ShieldsThere's tears and tantrums in this humorous picture book when Ellie is tired of playing with her toys. Ellie has everything, but she wants more. She wants an elephant. And she won't eat or sleep or brush her hair until she gets one! But when she finally gets her wish, the elephant decides to teach Ellie a thing or two about manners!
About the Author
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Book Info
32 pages
Gillian Shields
More books by Gillian Shields
Author's Website
Hodder Children's Books an imprint of Hachette Children's Group
Publication date
5th July 2012
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Welcome! I'm so glad you stopped by to visit my blog today and that you want to know a little more about me. I mean…that is why you're on this page, right? 😉 Honestly, for whatever reason I have the HARDEST time writing a bio post. It's seriously the hardest thing for me to ever write. I know that sounds ridiculous. It should be a piece of cake considering I am the authority on all things "me" related. How hard can it be to jot down a couple of paragraphs describing myself? Well, I'll tell you…it's very hard. But, it must be done so here's what I came up with:
First of all, my name is Nancy Hoeninghaus. I named this blog "From Haus to Home" as a play on words using the last part of my last name as it is pronounced like the word "house". Get it? See what I did there? Pretty clever, huh? 😉 I am married to a man I met when I was just 14 years old and have been with him now for more than half of my life. He is truly my very best friend, my biggest supporter, and my soft place to fall. We have been through the wringer together and I couldn't ask for a better person to do life with.
We have two amazingly brilliant and beautiful children, Isaiah (aka "Zai" aka "the boy child") and Alexa (aka "Lexi" aka" the girl child") . They are truly the best part of me and have brought more meaning to my life than I could've ever imagined.
I love singing (my mom says I've been singing since I could talk) and my greatest joy is singing worship songs to my Jesus! He is the reason I sing, the reason I breathe, the reason I thrive in life instead of just survive. Without Him, I don't even want to imagine where or who I'd be. He is my everything!!
I love to decorate and became a Certified Interior Decorator a couple of years ago. I mainly do it for fun and for friends. I really don't consider it my career.
Writing is my catharsis. It is the way I vent…the way I discover…the way I am inspired…the way I inspire. I love to write. I received my first diary as a gift when I was 9 years old and have written my feelings and ponderings on paper since then. Blogging just seemed like a perfect outlet for my love of writing and so here I am.
My life has been far from "normal" and I have been told by more people than I can count that I should have either a TV mini-series, a book, or most recently, a reality show because there are things I have experienced that just aren't "normal". I suppose those people find my history fascinating…to me it's just my life. Between my husband and my stories…we'd have books for days!! Haha! 🙂
I love inspiring others and hope that this blog will be just that…inspiring. Whether I'm blogging about a decorating or DIY project or life in general…my goal is to inspire, motivate, and encourage. I hope whatever you encountered on my blog today did one of those three things for you!
If you want to know more about me…just ask. I'm a pretty open book. 😉 I hope you peruse my blog and enjoy it! Happy reading!!
Oh, and I couldn't publish this page without a pic of our furry Hoeninghaus…Kevin!!


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Photo jewelry
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Learn to make Photo Jewelry from the Pro!//////// have been looking for a locket maybe I'll read this and try
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Hotel Coolgardie
This wryly observed documentary is set in a tiny mining town somewhere between Kalgoorlie and Perth. Two Finnish girls on working visas, Lina and Steph, head to this remote spot for a three-month stint behind the bar of the local pub. It's a tough gig. The girls contend with drunkenness, relentless sexual advances, and a belittling, bullying boss. The booze-addled patrons rant about failed relationships and female duplicity; it seems the girls' only ally is the toothless, odorous local, Canman. The barmaids struggle to negotiate this unhealthy environment, with little by way of distraction.
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★★★★★ review by Matthew Murray on Letterboxd
Every bit as fascinating as it is terribly uncomfortable, Hotel Coolgardie offers a unique insight into the behaviour of the sexually starved, oafish men of a small Australian mining town.
These men are gross. The feelings of repulsion quickly give way to pity, however, as the doco slowly reveals the extent of their desperation and loneliness. That same pity then shifts straight back to repulsion ss the residents behaviour gets worse. I was genuinely fascinated by this one, though there is often an uncomfortable tension that comes with watching two young women navigate through a territory full of unashamed brutes.
I'd definitely recommend this, as long as you can handle a little glimpse into uncivilised civilisation.
★★★★ review by michail on Letterboxd
In a sentence, I could smell the beer breath while watching this film. It was so uncomfortable to see the girls get harassed as much as they were (even though there were some really nice moments). But I think the best quality about this film is that it's so personal and up close, with seemingly invisible camerawork. A really great example example of observation documentary that made me feel like I was there with these girls on their journey. So engaging because it was just so real.
★★★½ review by ReedRothchild on Letterboxd
Hotel Coolgardie follows a pair of Finnish backpackers as they try and save up money by working as bar-staff in rural WA. That the bar happens to be THE WORST PLACE ON EARTH quickly becomes apparent. Subject to misogynistic abuse from their boss and patrons, they try their best to survive by befriending locals (many of whom are awful) and making the most of their time there. The film shows how loneliness and isolation can fuel bigotry and even though the film never condones the actions of the people of Coolgardie, it is not hard to see how they became the way they are. I was a little confused about the circumstances under which this doco was filmed as there is definitely a cameraperson but nobody acknowledges them and there are many car trips in which they are not present.
★★★★ review by Andy Diep on Letterboxd
A tip for the Australian Government: instead of spendingmillions of taxpayer money on anti-immigration online campaigns and telling everyone you're gonna stop the boats...
Just show them Hotel Coolgardie
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Staying at this hotel for one night very clean very tidy fantastic atmosphere. Staff are amazing will go out of the way to do anything for you view was amazing room was tidy clean very big when I did get lost jasmine went out of...More
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Green Coffee Bean Extract is Well-Liked in Vatican City, Nonetheless Green Coffee Bean Extract is Very Tough to Find in Local Stores in Vatican City
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Two Long Island, NY, beaches were named among the best in the country this year. Coopers Beach in Southampton, NY, ranked #1, while East Hampton's Main Beach took the #5 spot in the recently released 20th Annual Top 10 Beaches list, produced by Dr. Stephen P. Leatherman, director of Florida International University's Laboratory for Coastal Research.
1. Coopers Beach
Southampton, New York
2. Siesta Beach
Sarasota, Florida
3. Coronado Beach
San Diego, California
4. Cape Hatteras
Outer Banks of North Carolina
5. Main Beach
East Hampton, New York
6. Kahanamoku Beach
Waikiki, Oahu, Hawaii
7. Coast Guard Beach
Cape Cod, Massachusetts
8. Beachwalker Park
Kiawah Island, South Carolina
9. Hamoa Beach
Maui, Hawaii
10. Cape Florida State Park
Key Biscayne, Florida
Coopers Beach, on the east end of Long Island, New York, in Suffolk County, is located at 268 Meadow Lane near the intersection of Coopers Neck Lane in the Village of Southampton. At just about 500 feet long, it's part of a longer seven-mile stretch of white sand beaches in Southampton. Coopers Beach and Main Beach in East Hampton cach compose a small part of the more than 100 miles of oceanfront that runs along Long Island's South Shore.
Southampton, in the world-famous Hamptons region, is known for its quaint villages, miles of farmland, wineries, art, history, fine dining, and lodging. It is steeped in a history dating back to 1640. Parking is pricey ($40 daily) so the best way to visit the beach at no charge is a short bicycle ride from the Village of Southampton.
"This is really a recognition of the people in our community who respect the beach and take care of it; it's a reflection of our community," said Mark Epley, Mayor of Southampton Village. "I'm proud of all our beaches; they are raked and cleaned on a daily basis. Coopers Beach is a large white sand beach, well-maintained, with great dunes and beach grass that surround it. We have visitors come from all over the world. On any day in August, you can probably hear 15 different languages being spoken on the beach."
The Long Island Convention & Visitors Bureau anticipates that visitors and residents will be inspired to visit Long Island's other stretches of white sand public beaches, as well as its historic sites, hiking trails, golf, wineries, villages, restaurants, and of course, hotels.
For a free Travel Guide, call the Long Island Convention & Visitors Bureau at 1-877-386-6654 or visit www.discoverlongisland.com.
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Stoic Thrive Spark Tight
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Your alarm clock has four legs and a wet nose, and never fails to go off at 6:12 a. m. As you head out in the pre-dawn chill with your pooch, your Stoic Women's Thrive Spark Tight's stretchy, heavyweight fabric keeps your legs from freezing; as you start to wake up and warm up, it supports your muscles and keeps you comfortable by breathing and wicking moisture. You lengthen your stride on the running trail, inspired by the blazing sunrise and the need to keep up with your energetic pup before he runs afoul of the local skunks ... again.
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The Stoic Thrive Spark Tight is not available from the stores we monitor. It was last seen February 22, 2016 at Backcountry.com.


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Atlanta Hawks Honor Robin Roberts During Game
Robin Roberts receives 'Be Greater Award' for making a difference in the community.
'This Week' Sunday Spotlight: Robin Roberts
GMA's Robin Roberts on her new book "Everybody's Got Something" and her 2012 interview with President Obama on same-sex marriage.
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10 Yoga Poses For Beginners #yoga #yogasequence #sequence #inspiration http://www.aloyoga.com
30 Yoga Poses You Really Need To Know #yoga #stretch #fitness
The popular workout you can do at home!
3 Barre Workouts to Tone Your arms, butt, and legs! All you need is a counter top or table!
Five easy ballet barre fitness moves from the Cody blog
The popular workout you can do at home!
If you're trying to burn calories and lose fat while also sculpting your body, give one of these at-home barre workouts that burn fat a try! Barre exercises include postures from ballet, yoga, and pilates, and while the moves are slight, they go a long way in strengthening your muscles for a lean, toned look. You don't have to be a dancer or ballerina to enjoy these workouts, and if you don't have a ballet bar kicking around your living room (HA!), you can use a chair or table top instead.
Try Pilates from the comfort of your living room with this easy workout video created for beginners. Hello, summer abs! | via @SparkPeople #fitness #exercise #core
Your Abs Will Burn After This Barre Workout- soooo this is basically the 20 minute pilates workout I've been doing for years...
Lindsay Ellingson's 10-minute lower body pilates workout—see the GIFs!


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Town Square
Girls Softball Registration is Now Open!
Original post made by Steve Renfro, Danville, on Oct 3, 2012
There are three divisions of play: White (recreational ball), Blue (developmental), and Red (competitive). There is no tryout necessary for White Division play.
Volunteer to coach your daughter's Red, Blue or White Team by completing a coaching application at the time of player registration at www.sportability.com/srvgal (select desired grade based division, then select the "Register a Coach" option). Red and Blue division coaches are needed ASAP and the cutoff date for submitting your application is October 18th!
Tryouts for the Red and Blue divisions are on November 10th & 11th, and December 1st & 2nd at San Ramon Valley High School. In order to reserve a Red/Blue Tryout session, your daughter must be registered for the upcoming 2013 SRVGAL season. Then a "Registration Confirmation" email will be sent to you with the Red/Blue Tryout Sign-Up link that must be completed to reserve your daughter's tryout session. There are only 40 spots per tryout session and the cutoff date to register for these divisions is November 2nd, so sign up soon.
You can also sign up for the Pre-Tryout Skills Clinic at www.srvgalacademy.org. This clinic is designed to help your daughter become familiar with the tryout process and to work on the skills to make her more successful. These sessions fill up fast and are on a first come first serve basis!
Become a Team Sponsor by sponsoring your daughter's team. SRVGAL would not exist without the generosity and support of our sponsors. The sponsor fee is only $325 if paid prior to December 31st and includes your company name on the back of the jerseys as well as free advertisement for your business. For your convenience, you can pay online when you register your daughter. You will find a sponsor form on our website with additional information.
Thanks for your continued support of SRVGAL and feel free to e-mail the League Commissioner, Anne Phillips at firstname.lastname@example.com with any questions.
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Reporting from the scene is our contact, guY romagnali in Switzerland — he is with Daniel and Cumali, who organize the Mini Winter Rally.
These are just very short teaser videos, but you can view more videos (including footage from last year's Mini WInter Rally and IMM) on his YouTube page! 😉
Impressed with the coverage from Miniology thus far, he shares with us current news: A whopping total of 46 cars are registered from all across Europe, and the rally will start this Saturday. Here we have two short behind-the-scenes videos for faithful viewers, a "making of" — what we are seeing is shot directly from the interior of their MINI while they are preparing the road-course turn-by-turn maps, and you will see a quick shot of this year's roadbook cover as well.. enjoy! You can see that the driver (on left) is driving the road-course while the navigator (on right) is checking over the maps — this is the sort of preparation needed to make the event a great success for all the participants..
Video 1 – Mini Winter Rally The making of the Roadbook 2010 (Part 1)
Video 2 – Mini WInter Rally The making of the Roadbook 2010 (Part 2)
More videos and pics coming soon!
So you can get an idea of the turn out for these events, here is a quick video showing a pan of last year's run group – cars from Switzerland, France, Belgium, Italy, and elsewhere — 36 cars in all — look at all the classics in the snow! Looks like a lot of fun eh?
Video 3 – 2009 Mini WInter Rally (participants)
For more video snippets from last years rally (including lots of starting footage with Minis in the snow) check his YouTube as previously mentioned 😉


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Donington Performance
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Pseudomonas fluorescens is present in low number in the intestinal lumen and has been proposed to play a role in Crohn's disease (CD). Indeed, a highly specific antigen, I2, has been detected in CD patients and correlated to the severity of the disease. We aimed to determine whether P. fluorescens was able to adhere to human intestinal epithelial cells (IECs), induce cytotoxicity and activate a proinflammatory response.
Behaviour of the clinical strain P. fluorescens MFN1032 was compared to that of the psychrotrophic strain P. fluorescens MF37 and the opportunistic pathogen P. aeruginosa PAO1. Both strains of P. fluorescens were found to adhere on Caco-2/TC7 and HT-29 cells. Their cytotoxicity towards these two cell lines determined by LDH release assays was dose-dependent and higher for the clinical strain MFN1032 than for MF37 but lower than P. aeruginosa PAO1. The two strains of P. fluorescens also induced IL-8 secretion by Caco-2/TC7 and HT-29 cells via the AP-1 signaling pathway whereas P. aeruginosa PAO1 potentially used the NF-κB pathway.
The present work shows, for the first time, that P. fluorescens MFN1032 is able to adhere to IECs, exert cytotoxic effects and induce a proinflammatory reaction. Our results are consistent with a possible contribution of P. fluorescens in CD and could explain the presence of specific antibodies against this bacterium in the blood of patients.
Pseudomonas aeruginosa is an opportunistic pathogen frequently emerging from the mucosa-associated intestinal microbiota, which can cause severe septicemia in immuno-compromised hosts. Several interaction mechanisms of P. aeruginosa with intestinal epithelial cells (IECs), especially adhesion and penetration, have been studied in detail [1-3]. Conversely, little attention has been given to other species of the same genus, like Pseudomonas fluorescens.
Pseudomonas fluorescens has long been considered as a psychrotrophic microorganism, unable to grow at temperatures over 32°C, however we have recently shown that some strains isolated from a clinical environment are able to grow at or above 37°C [4]. P. fluorescens is a widespread gram-negative bacterium present in a variety of ecological niches such as refrigerated food products, soil, water [5] and in the digestive tract [6]. Interestingly, a highly specific antigen of P. fluorescens, designated as I2, was detected in the serum of 54% of the patients suffering from ileal Crohn's disease (CD) [7] and a direct link between the severity of the pathology and the level of circulating I2 antigen has been demonstrated [8]. Surprisingly, the proinflammatory potential of this bacterium or its interaction with the intestinal epithelium has never been investigated.
Several studies have focused on the mucosal immune response to pathogenic bacteria. Human IECs infected with pathogenic bacteria generally produce proinflammatory cytokines, such as interleukin (IL)-8 [9]. The latter has a chemotactic role and can recruit polymorphonuclear cells into the infected site and promote their infiltration of the epithelial layer infected by invasive or noninvasive bacteria [10,11]. IL-8 gene expression is regulated by two major transcriptional factors: nuclear factor kappa B (NF-κB) and activator protein (AP)-1 [12]. NF-κB has a pivotal role in the immune and inflammatory response, but also controls cell survival, proliferation and differentiation [13,14]. Recent works demonstrated that NF-κB signaling is a critical element of the homeostatic immuno-inflammatory function in the gut. Indeed, epithelial NF-κB preserves the integrity of the gut epithelial barrier and coordinates the antimicrobial actions of the innate and adaptive immune systems [15]. Nevertheless, hyperactivation of this transcription factor results in chronic inflammatory bowel diseases [16]. Activation of AP-1 is dependent on mitogen-activated protein kinases (MAPK) that are central in many physiological processes, including regulation of cytokine and stress responses and cytoskeletal reorganization [17,18].
P. fluorescens MFN1032 is a clinical strain recently isolated in our laboratory [19]. It displays hemolytic activity toward sheep erythrocytes [20,21], however, its infectious potential on human IECs is still unknown.
In the present study, we investigated adhesion and cytotoxic properties of P. fluorescens MFN1032 on Caco-2/TC7 and HT-29 cell lines in comparison to the psychrotrophic strain, P. fluorescens MF37 and the well-known opportunist pathogen P. aeruginosa PAO1. The proinflammatory potential of P. fluorescens MFN1032 was also evaluated by the measurement of IL-8 secretion on both Caco-2/TC7 and HT-29 cells, and analysis of NF-κB and AP-1 activation using the reporter gene strategy.
The binding index of the clinical strain P. fluorescens MFN1032 on Caco-2/TC7 and HT-29 cells was determined after 5 h of incubation and compared to P. fluorescens MF37 and P. aeruginosa PAO1. The data presented in Figure Figure11 show that these bacterial strains adhere to both cell lines but the binding index was higher for Caco-2/TC7 (Figure (Figure1A)1A) than for HT-29 (Figure (Figure1B1B).
P. aeruginosa PAO1 showed the highest adhesion potential on Caco-2/TC7 cells compared to P. fluorescens MF37 and P. fluorescens MFN1032. When the cells were infected with a 106 CFU or 108 CFU ml-1 bacterial solution, the mean adhesion index of P. aeruginosa PAO1 reached 12.6 ± 2.6 or 32.1 ± 1.9 bacteria cell-1, respectively, whereas the adhesion of P. fluorescens was quite similar for the two strains with 10.6 ± 0.5 or 18.1 ± 1.9 bacteria cell-1 and 8.2 ± 0.6 or 19.8 ± 2 bacteria cell-1 for MF37 and MFN1032, respectively.
The same experiment using HT-29 cells showed that the binding index of P. aeruginosa PAO1 remained the highest (7.1 ± 0.8 or 10.1 ± 1.0 bacteria cell-1) but the index of P. fluorescens MFN1032 (4.3 ± 0.6 or 8.3 ± 1.6 bacteria cell-1) was significantly higher than that of MF37 (1.4 ± 0.2 or 2.3 ± 0.5 bacteria cell-1).
The cytotoxic effect of Pseudomonas strains on Caco-2/TC7 and HT-29 cells was determined by quantification of lactate dehydrogenase (LDH) released in culture medium (Figure (Figure22).
P. fluorescens MF37 exhibited the lowest cytotoxic activity (expressed as % of maximal LDH release) with only 7.8 ± 1.9% (at 106 CFU ml-1) or 30 ± 16.4% (at 108 CFU ml-1) of cell lysis after 24 h of infection on Caco-2/TC7 (Figure (Figure2A)2A) and 17.5 ± 1.1% (at 106 CFU ml-1) or 22 ± 2.0% (at 108 CFU ml-1) of cell lysis for HT-29 cells (Figure (Figure2B).2B). The cytotoxicity of MFN1032 was higher with 34 ± 15.2% or 74.7 ± 4.6% lysis for infection respectively with 106 or 108 CFU ml-1 on Caco-2/TC7 and 33.2 ± 1.5 or 60.3 ± 5.5% lysis after infection with 106 or 108 CFU ml-1 respectively on HT-29. P. aeruginosa PAO1 led to a total lysis of Caco-2/TC7 at the two bacterial concentrations tested and on HT-29, with infection rates of 106 or 108 CFU ml-1, LDH release was 67.9 ± 7.2% or 85.6 ± 3.4% respectively. At the end of infection, Caco-2/TC7 and HT-29 cells were observed by light microscopy. Figure Figure33 shows the cell monolayers after infection with MOI of 100 (108 CFU ml-1). When Caco-2/TC7 cells where infected with P. fluorescens MF37, a slight cell detachment was detectable while more cells were detaching after infection with MFN1032. Infection with P. aeruginosa PAO1 led to a complete disappearance of the organized Caco-2/TC7 and HT-29 monolayers.
The bacterial proinflammatory effect was assessed by measuring IL-8 secretion. Compared to untreated cells, the three Pseudomonas strains induced significant stimulation of IL-8 secretion in both Caco-2/TC7 (Figure (Figure4A)4A) and HT-29 cells (Figure (Figure4B).4B). Mean values of IL-8 on HT-29 and Caco-2 in response to P. fluorescens MF37 and MFN1032 were similar for these two strains and it is noteworthy that IL-8 secretion was significantly increased in HT-29 compared to Caco-2 cells.
To further explore the immuno-modulatory properties of P. fluorescens MFN1032, we tested the effects of this bacterium on NF-κB or AP-1 activation using Caco-2 and HT-29 reporter cell lines. We observed that P. aeruginosa PAO1 stimulated NF-κB activity by 2.5-fold over control in both Caco-2/κb-seap-7 and HT-29/κb-seap-25 reporter clones (Figure (Figure5)5) while it had no effect on the AP-1 pathway (Figure (Figure6).6). Interestingly, P. fluorescens MF37 and MFN1032 had an opposite effect. Indeed, none of these strains induced NF-κB activation (Figure (Figure5)5) whereas they both activated the AP-1 pathway by 2.2-fold over control in Caco-2/ap1-luc-1 and HT-29/ap1-luc-6 reporter clones (Figure (Figure66).
P. fluorescens is present at low level in the human gut and has been linked to Crohn's disease (CD) [7,8], however little is known about the potential interaction of this bacterium with the intestinal mucosa. In the present paper, we aimed at determining its potential to adhere to IEC, to induce cell cytotoxicity and trigger a proinflammatory response. We selected two strains, a classical psychrotrophic strain (MF37) and a recently characterized clinical strain adapted to grow at 37°C (MFN1032). The behaviour of these bacteria was compared to that of the opportunistic pathogen P. aeruginosa.
Since adhesion and cytotoxicity to IECs are crucial events in the infection process, the three strains were tested on two epithelial cell lines. Except for adhesion, the two IECs models used in this study gave similar responses to the three strains of Pseudomonas. Indeed, a dose dependent adhesion of bacteria to Caco-2/TC7 and HT-29 cells was observed with the greatest effect obtained with the opportunistic pathogen P. aeruginosa. It is noteworthy that, compared to the psychrotrophic strain MF37, the clinical strain P. fluorescens MFN1032, which is adapted to develop at 37°C displayed statistically significant higher adhesion potential to HT-29 but not to Caco-2/TC7 cells. This observation suggests that the clinical strain may express a greater diversity of adhesion factors than MF37 and could explain, at least in part, the higher cytotoxicity effect of MFN1032.
Although differences exist between surface proteins expressed by Caco-2/TC7 and HT-29 cell lines in comparison to normal human IECs, our results support the hypothesis that P. fluorescens should be able to colonize the intestinal mucosa. Pseudomonad are rarely searched for and detected as fecal bacteria, and are usually considered as a sub-dominant population [22]. In addition, there is now ample evidence that the circulating bacterial population in the intestinal lumen is very different from the resident microbiota that comes in contact with the apical surface of the enterocytes and is tightly associated to the mucus/glycocalyx layer [23,24]. For an aerobic bacterium such as P. fluorescens, the best ecological niche should be at the vicinity of the epithelium, where oxygen concentration is the highest in the intestinal environment [25]. This is supported by the evidence showing that the P. fluorescens-specific I2 antigen sequence is systematically detected in ileal mucosa samples [7]. Moreover, in CD patients, there is a positive correlation between blood level of circulating anti-I2 antibodies and the severity of the disease [8] suggesting that the I2-producing bacteria, i.e. P. fluorescens, are in close contact with enterocytes and could contribute to CD pathogenesis.
The LDH release assay showed that the cytotoxicity of P. fluorescens on Caco-2/TC7 and HT-29 cells is lower than that of P. aeruginosa. This limited virulence of the P. fluorescens strains seems to be normal for a species that should be a resident in the intestine whereas P. aeruginosa is typically an opportunistic pathogen only detected in case of declared infection [26]. This hypothesis is also in agreement with the hierarchy of the cytotoxic activity of the two tested strains of P. fluorescens, the clinical strain MFN1032 being more virulent than the environmental and psychrotrophic strain MF37. Bacterial cytotoxicity is a highly complex phenomenon combining the virulence of the prokaryote and the intrinsic sensitivity of the eukaryotic cell. In opposition to the present results, Chapalain et al found that the cytotoxic activity on glial cells was higher for P. fluorescens MF37 than MFN1032 [4]. These observations are in agreement with the work of Picot et al showing that in the case of P. fluorescens, the necrotic and apoptotic activities are not simply correlated to the adhesion potential of the strain [27].
In contrast to P. aeruginosa, the proinflammatory effect of P. fluorescens strains has not been elucidated. In this study, we demonstrated that similarly to P. aeruginosa, P. fluorescens MFN1032 and MF37 exerted a direct proinflammatory effect on IECs as demonstrated by induction of IL-8 secretion. The homogenous proinflammatory response of IECs induced by the two P. fluorescens strains studied suggests a link between the proinflammatory properties and a common pathogenic factor of these strains. IL-8 gene expression is regulated by several signaling pathways including mainly NF-κB and AP-1 transcription factors. Previous studies have shown that P. aeruginosa activates NF-κB in mouse monocyte/macrophage cell line [28] and MAPK signaling pathways in lung epithelial cells [29], which in turn leads to the production of proinflammatory cytokines, such as IL-6, IL-8, and TNF-α (tumor necrosis factor alpha). In our study, the two P. fluorescens strains failed to activate the NF-κB pathway in contrast to P. aeruginosa, however the two strains were able to activate AP-1 signaling, suggesting that the proinflammatory effect of these bacteria in IECs is linked to the activation of MAPK signaling pathways. The MAPK form a group of three pathways, including extracellular signal-regulated protein kinases (ERK1/2) and two stress-activated protein kinases designated p38 and JNK (c-jun N-terminal kinase) [30]. The activation of MAPK has been reported to be involved in response to infection by invasive bacteria, such as Salmonella enterica serovar typhimurium or Listeria monocytogenes, in IECs [31,32] or in macrophages [33]. Moreover, it has been shown that enteroadherent Escherichia coli activate this pathway and both bacterial attachment and secreted proteins might be implicated in cytokine responses [34]. P. aeruginosa as well as P. fluorescens contain multiple cell surface factors, including flagellin, pili, LPS, type III-mediated toxin secretion and quorum-sensing molecules which could interact with distinct epithelial membrane proteins, such as asialylated glycolipid receptors, toll like receptors (TLRs), or combinations of these proteins [28,35,36]. We have shown that purified flagellin strongly activated NF-κB pathway in HT-29 and to a lower extent in Caco-2, whereas both cell lines poorly responded to LPS (Lakhdari et al, submitted manuscript). In contrast, purified flagellin and LPS do not activated the AP-1 pathway in the two cell lines (data not shown). Thus, we can conclude that P. fluorescens activated AP-1 pathway in Caco-2 and HT-29 independently of flagellin and LPS expression. Further investigations will be needed to identify the exact nature and function of P. fluorescens compounds responsible for MAPK activation in IECs.
P. fluorescens MFN1032, P. fluorescens MF37 and P. aeruginosa PAO1 were found to adhere to Caco-2/TC7 and HT-29 cells and the cytotoxicity towards these cell lines was higher for the clinical strain MFN1032 than for MF37. We showed that the two strains of P. fluorescens induced IL-8 secretion by Caco-2/TC7 and HT-29 cells via the AP-1 signaling pathway whereas P. aeruginosa PAO1 potentially used the NF-κB pathway. To our knowledge, this work is the first to demonstrate the interaction and the proinflammatory potential of P. fluorescens on IECs.
The human colon adenocarcinoma cell lines Caco-2/TC7 [37] and HT-29 were used between passages 10 and 35. Caco-2/TC7 cells were grown in Dulbecco's modified Eagle Minimal Essential Medium (Sigma) containing 20% foetal calf serum (FCS) supplemented with 2 mM of L-glutamine, 100 U ml-1 each of penicillin and streptomycin and 1% non-essential amino acids at 37°C with 5% CO2. HT-29 cells were grown in Dulbecco's modified Eagle Minimal Essential Medium (Sigma) containing 10% FCS supplemented with 2 mM of L-glutamine, 100 U ml-1 each of penicillin and streptomycin at 37°C with 5% CO2.
P. fluorescens MF37 is a rifampicin-resistant natural mutant of the strain MF0 (Biovar V), originally identified in crude milk [38]. P. fluorescens MFN1032, is a clinical biovar I strain collected in a hospital of Haute-Normandie (France) [4]. P. aeruginosa PAO1 was obtained from an international collection. Bacteria were grown overnight in ordinary nutrient broth (Merck) at 28°C for the two strains of P. fluorescens and at 37°C for P. aeruginosa PAO1. For adhesion and cytotoxicity assays, bacteria in stationary phase were harvested by centrifugation (5000 × g, 5 min, 20°C) and resuspended in antibiotic-free and serum-free cell culture media at densities of 106 and 108 CFU ml-1, corresponding to a multiplicity of infection (MOI) of 1 and 100 respectively.
For adhesion assays, Caco-2/TC7 and HT-29 cells were seeded at a concentration of 1 × 105 cells ml-1 on coverslips coated with 50 μg ml-1 poly-L-lysine and used at 80% confluence as recommended by Li et al [39]. Cells were incubated for 5 h with 1 ml of the bacterial suspensions. After incubation, the medium and non-adherent bacteria were removed by washing. Then, the coverslips were fixed with methanol (10 min), stained with Giemsa solution (20 min) and observed using an Axiovert S100TM light microscope (Zeiss). The adhesion index (mean number of bacteria adherent per cell) was determined by direct counting on a minimum of 100 cells following the technique of Darfeuille-Michaud et al [40].
Confluent Caco-2/TC7 and HT-29 cells cultivated in 24-well culture plates were infected for 24 h with 1 ml of the bacterial suspensions. At the end of incubation, lactate dehydrogenase (LDH) present in the supernatant was measured in each well using the Cytotox 96R enzymatic assay (Promega). LDH is a stable cytosolic enzyme released by eukaryotic cells and an overall indicator of necrosis. Caco-2/TC7 and HT-29 cells exposed to Triton X100 (0.9%) were used as a control of total release (100% LDH release). The background level (0% LDH release) was determined with serum free culture medium. The percentage of cytotoxicity was calculated following the manufacturer's instructions.
IL-8 assays were performed on confluent Caco-2/TC7 and HT-29 cells monolayers grown in 24-well culture plates. After 24 h of infection with the bacterial suspensions (MOI of 100), immunoreactive IL-8 protein levels in cell culture supernatant were quantified using an ELISA Quantikine kit (R&D systems) according to the manufacturer's protocol.
The NF-κB reporter clones Caco-2/κb-seap-7 and HT-29/κb-seap-25 were obtained after a stable transfection of parental cells with the reporter plasmid pNiFty2-SEAP (Invivogen), which contains SEAP (secreted alkaline phosphatase) as reporter gene downstream of five repeats of the NF-κB binding consensus.
The AP-1 reporter clones Caco-2/ap1-luc-1 and HT-29/ap1-luc-6 were obtained after a stable co-transfection of the reporter plasmid pAP-1-luc (Stratagen), which contains luciferase as reporter gene downstream of seven repeats of the AP-1 binding consensus, together with pTK-Hyg (Clonetech) a hygromycine-based selection vector. Transfection of HT-29 was performed by lipofection using TFX-50 (Promega) according to the manufacturer's instructions while Caco-2 cells were transfected using the Amaxa Nucleofector system (Lonza).
For each experiment, reporter cells were seeded at 50 000 cells per well, into 96-well plates and pre-incubated 24 hours before adding live bacteria at an MOI of 100.
For NF-κB activation assays, Caco-2/κb-seap-7 and HT-29/κb-seap-25 cells were incubated with live bacteria for 8 hours and IL-1β (10 ng/ml) was used as a positive control. SEAP activity in the supernatant was measured using the Quanti-Blue reagent (Invivogen) using the manufacturer's protocol and quantified as OD at 655 nm.
For AP-1 activation assays, cells were incubated for 12 h with live bacteria. Phorbol-myristate -acetate (PMA, 1 μM) or butyric acid (2 mM) was used as a positive control for Caco-2/ap1-luc-1 or HT-29/ap1-luc-6 reporter cells respectively. Luciferase activity was measured using the ONE-Glo™. Luciferase Assay System (Promega) according to the manufacturer's instructions and quantified as relative luminescence units (RLU).
All measurements were performed using a microplate reader (Infinite 200, Tecan).
Data are expressed as a mean ± standard error (SEM) calculated over three independent experiments performed in triplicate. Analysis of statistical significance were performed by ANOVA with Bonferroni post hoc test (adhesion and cytotoxicity assays) or Student's t-test (IL-8 secretion, NF-κB and AP-1 activation assays)
AM carried out most experiments and analyzed most of the data. NC wrote the manuscript, participated in the design of the study and analyzed most of the data. MG carried out the IL-8 ELISA assay. OL carried out the construction of NF-κB reporter cells. KR carried out the construction of AP-1 reporter cells. JD and HB participated in the design of the construction of NF-κB and AP-1 reporter cells and help to draft the manuscript. PS and NO were involved in the design of the study. MF participated in the design of the study and writing of the manuscript, AG performed the statistical analysis. All authors read and approved the final manuscript.
This work was supported by a BRI grant (Bourse Régionale Industrielle) from the Région Haute-Normandie and BIOGALENYS. OL is supported by the European Community's Seventh Framework Programme (FP7/2007-2013): MetaHIT, grant agreement HEALTH-F4-2007-201052. We thank Mihai Covasa and Christine Farmer for revising the English manuscript.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Incy Wincy Spider
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All posts tagged "featured"
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Quarto (LSE: QRT.L),The world's leading global illustrated book publisher and distribution group, today announces that it has entered into a joint venture agreement with Sharjah based Kalimat Group for Arab language books.
Imprints from around Quarto's publishing businesses will supply titles to the joint venture that will publish and distribute them throughout the Middle East and North Africa from early 2017. The new joint venture will publish under the Kalimat Quarto imprint starting in cookery, a new category of publishing for Kalimat Group. Other categories, including children's books, are envisaged as the co-operation grows.
Quarto and Kalimat see great scope for developing the market for illustrated non-fiction in the UAE and the larger Arabic speaking world. Currently this category is dominated by English language imports, which excludes a huge potential readership as English proficiency in the Arabic speaking world is still low.
Sheikha Bodour bint Sultan Al Qasimi, Founder and CEO of Kalimat Group, said: "This MoU represents an exciting opportunity for Kalimat Group to expand its offerings in what is a new segment of publishing for the house. Kalimat Group has led the illustrated book field in other categories with its offerings garnering a number of prestigious international awards. We are delighted to be able to bring the group's talents in narrative illustration to a new line in book publishing and intend to build upon our incipient relationship with Quarto to take our organisation to new heights."
Marcus Leaver, Chief Executive of Quarto, said: "Our stated intention to develop our business internationally sees a further iteration in the Arabic speaking world. A partnership with such a pioneering publisher as Kalimat Group and the launch of Kalimat Quarto is exciting and raises the bar yet again for further partnerships in other territories."
GMT 12:21 2017 Thursday ,11 MayIraq presents 40 pieces of different phases of civilization
GMT 12:08 2017 Wednesday ,10 MayArcheological tomb is discovered in Minya governorate
GMT 10:16 2017 Monday ,08 MayMusical play about Iraq's war opens in New York
GMT 09:38 2017 Saturday ,06 May'Burden': Portrait of the artist as a gunshot victim
GMT 07:58 2017 Friday ,05 MaySaudi artists seen 'pushing' boundaries
GMT 08:29 2017 Thursday ,04 MayExhibition of AFP migrant crisis photos opens
GMT 13:21 2017 Wednesday ,03 MayCelebrating Strength in Working Together, Cherishing Diversity
GMT 08:11 2017 Wednesday ,03 MayChina's military pact with N.Korea looks shaky


text:
donald_trump 1183
When the World Is Led by a Child - The New York Times
yesterday by jerryking
David Brooks MAY 15, 2017David_Brooks Donald_Trump immaturity self-discipline self-awareness sociopaths narcissism impulse_control letters_to_the_editor
-- "Trump is an infantalist" (or as most of call it, childish)
-- Trump's "falsehoods are attempts to build a world in which he can feel good"
-- He "is too incompetent to understand his own incompetence"
-- He "wants people to love him"
-- "there is perpetually less to Trump than it appears"
-- the Russian leak revealed Trump's"dangerousness"
Please, media, stop analyzing Trump's psychological makeup. He is the impulsive narcissistic you see. He really does think he is owed a "pledge of loyalty". He really does think he's smarter than the Generals, than scientists, experts, academics. He really does think he's owed constant adulation. He really is as hollow as he seems.
-- "Trump is an infantalist" (or as most of call it, childish)
-- Trump's "falsehoods are attempts to build a world in which he can feel good"
-- He "is too incompetent to understand his own incompetence"
-- He "wants people to love him"
-- "there is perpetually less to Trump than it appears"
-- the Russian leak revealed Trump's"dangerousness"
Please, media, stop analyzing Trump's psychological makeup. He is the impulsive narcissistic you see. He really does think he is owed a "pledge of loyalty". He really does think he's smarter than the Generals, than scientists, experts, academics. He really does think he's owed constant adulation. He really is as hollow as he seems.
yesterday by jerryking
Trump and the problem with the new normal
4 days ago by jerryking
Twenty years ago, Nasa scientists asked the sociologist Diane Vaughan to study the causes of the 1986 Challenger space shuttle disaster. Vaughan responded by developing a concept she called "the norma...Gillian_Tett Donald_Trump NASA deviance normality White_House complacency normalization from notes
4 days ago by jerryking
I wrote 'The Art of the Deal' with Trump. His self-sabotage is rooted in his past. - The Washington Post
6 days ago by paniedejmirade
The Trump I got to know had no deep ideological beliefs, nor any passionate feeling about anything but his immediate self-interest. He derives his sense of significance from conquests and accomplishments. "Can you believe it, Tony?" he would often say at the start of late-night conversations with me, going on to describe some new example of his brilliance. But the reassurance he got from even his biggest achievements was always ephemeral and unreliable — and that appears to include being elected president. Any addiction has a predictable pattern: The addict keeps chasing the high by upping the ante in an increasingly futile attempt to re-create the desired state. On the face of it, Trump has more opportunities now to feel significant and accomplished than almost any other human being on the planet. But that's like saying a heroin addict has his problem licked once he has free and continuous access to the drug. Trump also now has a far bigger and more public stage on which to fail and to feel unworthy.donald_trump politics usa
6 days ago by paniedejmirade
The Comey firing reminds us of a bigger danger - The Washington Post
8 days ago by jerryking
By Fareed Zakaria Opinion writer May 11Donald_Trump James_Comey firings democracies Fareed_Zakaria
8 days ago by jerryking
Donald Trump staffer found guilty of 10 counts of election fraud | The Independent
9 days ago by Psook
trump_related author:shehab_khan TrumpRussia Donald_Trump trump
A man who worked on Donald Trump's campaign in Michigan has been found guilty on 10 counts of election fraud.
Brandon Hall, a political activist, forged signatures on petition forms in 2012 and now faces up to five years in prison.
The 27-year-old from Grand Haven, along with his friend Zachary Savage, forged signatures in support of judicial candidate Chris Houtaling.
9 days ago by Psook
Transcript: Interview with Donald Trump
9 days ago by Psook
A rambling tirade from Donald J Trump, Preisdent of the United States and leader of the Republican Partyinterview trumponomics donald_trump economics trump the_economist
9 days ago by Psook
Trump's Expected Pick for Top USDA Scientist Is Not a Scientist
10 days ago by micahrobbins
Sam Clovis is expected to head the USDA's research section, which is a position reserved for "distinguished scientists with specialized or significant experience in agricultural research, education, and economics," but Clovis is not a scientist.Donald_Trump Sam_Clovis USDA science climate_change global_warming
10 days ago by micahrobbins
Russian photographer on White House outrage: 'This is nonsense' - CNN
11 days ago by Psook
Alexandr_Scherbak donald_trump Russian_photographer trump Sergey_Lavrov TrumpRussia Author:Euan_McKirdy
Reporters weren't allowed into the meeting, and the only images that emerged came from Russian government accounts, while Scherbak's images appeared in Russian state media and its social media accounts.
White House officials had allowed Scherbak, described by the delegation as Lavrov's photographer, to record the meeting, claiming they didn't know he also works for TASS.
"They tricked us," an angry White House official said.
"That's the problem with the Russians -- they lie," the official added.
"So: Russian state media had an exclusive on the Oval Office meeting from which American journalists had been barred," CNN commentator Frida Ghitis wrote.
A White House official said the administration did not anticipate that the Russian government would allow its state news agency to post photographs of the meeting.
11 days ago by Psook
Trump said he was thinking about the Russia investigation when he decided to fire FBI Director James Comey.
11 days ago by Psook
Updating summary of the James Comey firing and fallout thereof from Buzzfeed News.eli5 whitehouse donald_trump james_comey comeyfiring trump updating
11 days ago by Psook
related tags2010s 2016 2016_presidential_race 2017 @rollingstone @theatlantic abonnement aca afghanistan ajit_pai alexandr_scherbak america andrew_sullivan angela_merkel antisemitism asian-americans aspen author:euan_mckirdy author:shehab_khan authoritarianism autocracy basher_al-assad boeing bomb breitbart bureaucrats business business_interests campaign campaign_finance canada capitalism caucasians ceos china chrystia_freeland cities citizen class clichés climate_change coal comey comeyfiring comparison complacency congress connecting_the_dots conservatives conspiracies consulting conversation corey_lewandowski correction corruption crisis culture_wars daca damage dan_ozzi david_brooks davos deafness death debt deep_state defense_spending delusions democracies democracy democrat democratic_party democrats deportation desmond_cole destruction deviance diplomacy distance donald_rumsfeld dopost drug_prices eavesdropping economics election electoral_politics electric_cars eli5 elites elitism eric_posner ethnicity europe examination expense exxon facebook failure fareed_zakaria fb fbi fcc fda feed fever-swamp firings five_eyes foreign_policy fox_news gchq gene_park genocide german gillian_tett global_warming globalization gm golf government government_spending grand_strategy green guilt health_care health_insurance healthcare hillary_clinton history holocaust hubris humility ignorance igor_volsky image immaturity immigration impulse_control income influence infowars intelligence_agency internet interview investigation isps jacobinmag.com james_comey jane_mayer john_oliver joshua_barajas justin_trudeau kellyanne_conway killing koch konrad_yakabuski kyle_griffin letter letters_to_the_editor liberals-vs-left liberals lobbying lockheed lying mainstream_media matt_bruenig matt_karp media medicaid mexico michael_hayden middle-class mike_pence military mistake moab money nafta narcissism nasa national_interests nato nepotism new_jersey new_york_times news newshour nicholas_kristof nordstrom normality normalization npr nyc on_the_media op-ed paranoia paul_manafort pbs.org pentagon persuasion pharma phone politician politics populism postmodernism poverty presidency president pressekonferenz prime_minister privacy professional_service_firms progressivism public_policy public_relations public_sector q&a question quote race racism ralf_dahrendorf real_estate realpolitik reference regulation regulatory_capture republicans rex_tillerson right_wing_media robert_mercer ross_douthat russia russian_photographer s sam_clovis samantha_bee scandal scenario-planning science scott_adams screengrab sean_spicer security security_&_intelligence self-awareness self-discipline sergey_lavrov shame shock single_payer social_cohesion sociopaths state states statistic stephen_bannon stern steve_bannon strategy stupid suburb suburbia super_pacs syria takedown technology test the_economist thomas_cromwell trevor_noah tribalism trump trump_administration trump_related trumponomics trumprussia tudors turkey tweet twitter.com twitter u.s.foreign_policy united united_kingdom unknowns updating us_election us_politics usa usda vic_berger victor_davis_hanson video violence virtual_reality vote w wall_street_journal war war_party waste wealth weapon wef western_civilization white_flight white_house white_supremacy whitehouse whites work working_class writing
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Stoffel's Shady Oaks Resort
View Website Favorite 6512 State Road 70 - Siren, WI 54872
Information: 715-349-5725
You'll discover the perfect getaway vacation at Stoffel's Shady Oaks Resort whether fishing, tubing, skiing, golfing, or just relaxing with a good book. Shady Oaks Resort is a family-oriented resort tucked into an oak woods on the quiet shores of Lower Clam Lake. We offer six clean, 2-bedroom, non-smoking, and well-maintained accommodations ten minutes northeast of Siren, Wisconsin. We extend an invitation for your family to come, and enjoy the "Good Life" vacation that we have waiting for you.
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Nearby Places
Big Mike's Outdoor Sports Shop Siren - 0.35 miles away
Gandy Dancer State Trail - North Siren - 2.27 miles away
Burnett County Trails Information Siren - 2.27 miles away
Burnett County ATV Trails Siren - 2.32 miles away
Furs, Fins & Feathers Sports Siren - 2.62 miles away
Siren Antiques, Collectibles & Gifts Siren - 3.41 miles away
Making Memories & More Siren - 3.53 miles away
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More To Explore
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Casco Art Institute: Working for the Commons
On 3 April 2017 we announced Casco – Office for Art, Theory and Design's transition into Casco Art Institute: Working for the Commons. The transition involves the eponymous "exhibition" phase from 26 May–16 July 2017.
As we transition into an institute for the commons, our process-driven work is especially significant. While exhibitions are not always the best medium to unfold such a process, as display tends toward finality, we also see the possibilities within rearranging and taking pause to grow and entangle. It is an integral part of the commoning process and depends on your feedback and common desire to (un)learn!
Exhibiting institutes as artworks is not new—think Marcel Broodthaers' Musée d'Art Moderne, Département des Aigles, Section XIXème Siècle (1968), Thomas Hirschhorn's Musée Précaire Albinet (2004), and more recently Tania Bruguera's Museum of Arte Útil (2013) among many others. As such, our exhibition looks to this history, but actualizes the institute as artwork by ultimately making it into Casco Art Institute. That's also why we want to exhibit the aspects of institutes that are not usually exhibited—what we call the "body"—budget, relations, ethics, and so on.
This effort requires close collaboration with many, who are in fact "commoning" Casco and generating the commons, besides us, including: Adelita Husni-Bey, Aimée Zito Lema, Annette Krauss, Bram van den Berg, Charlotte Rooijackers, Cooperativa Cráter Invertido, David Bennewith, Dora García, Fernando García-Dory (Inland), Faivovich & Goldberg, Gunndís Ýr Finnbogadóttir, Ingo Niermann, Jort van der Laan, Laura Pappa, Lily van der Stokker, Lotte Schröder, Maja Bekan, Marjolijn Dijkman, Merel Zwarts, Riet Wijnen, Ruth Buchanan, Southern Wave (Dutch Art Institute) , Terra Critica (Utrecht University), and Wok the Rock. Some have developed long-term projects with us, now introduced from an "action" perspective, and others share their imaginings and methodologies for commoning.
Special Dates
26 May 2017
Exhibiting an institution, Casco Art Institute: Working for the Commons, newsletter #1
Who is Nina?, Casco Art Institute: Working for the Commons, newsletter #2
Optic to Haptic Cinema (OH Cinema), Casco Art Institute: Working for the Commons, newsletter #3
"Commoning is an art of the many", Casco Art Institute: Working for the Commons, newsletter #4
Press Release
Supported by
The Casco program is supported by Mondriaan Fund, the City Council of Utrecht, and DOEN Foundation via Arts Collaboratory.


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Town Square
Weekend, Aug. 6 & 7
Original post made on Aug 5, 2011
The Stanford Summer Chorus will be presenting a concert of early American choral music showcasing a variety of music from colonial times to the 20th century Saturday, Aug. 6, at 8 p.m. at Stanford Memorial Church, Stanford.
[Web Link ==B Theater:== Woman's Will at Rengstorff House]
Woman's Will, a Bay Area Shakespeare troupe, returns to the gardens of the Rengstorff House for a free performance of "A Midsummer Night's Dream" on Sunday, Aug. 7, 1-3 p.m. Rengstorff House at Shoreline, 3070 N. Shoreline Blvd., Mountain View.
[Web Link ==B Event:== Obon Bazaar]
The Palo Alto Buddhist Temple holds its annual Obon festival, featuring a silent auction, Japanese and American food, music, dance and cultural exhibits, this weekend, Aug. 6 and 7, from 5-10 p.m. Saturday and noon to 9 p.m. Sunday at Palo Alto Buddhist Temple, 2751 Louis Road, Palo Alto.
Read the full story here Web Link posted Friday, August 5, 2011, 11:59 AM
Comments (1)
a resident of Cuesta Park
on Aug 5, 2011 at 1:25 pm
Peninsula Youth Theatre's "Bye Bye Birdie" runs this weekend at the Mtn View Center for the Performing Arts--Friday at 7:30, Saturday at 2:00 and 7:30, and Sunday at 1:00. Tickets at the door or via www.pytnet.org
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Palo Alto: Bon Vivant to be replaced by bakery
By Elena Kadvany | 8 comments | 3,281 views
What's Wrong with This?
By Chandrama Anderson | 2 comments | 1,274 views
Don't forget to vote!
Don't forget to cast your ballot online. Voting ends May 29th. Stay tuned for the results in the July 21st issue of the Mountain View Voice.


text:
3.4/5 (68 %), 1 vote
Total number of Fun Time Arcade stores in Canada: 1
The biggest mall in Canada with Fun Time Arcade store: Rupert Square Mall
List of Fun Time Arcade stores locations in Canada. Find the closest Fun Time Arcade store in Canada Cities, Provinces and Territories.
You will get following information:
220 Yonge Street, Toronto, ON M5B 2H1213 stores
261055 CrossIron Blvd, Unit 800, Rocky View, Alberta T4A 0G3, Canada212 stores
66Q - 1485 Portage Avenue, Winnipeg, MB, R3G 0W4189 stores
109 Street & Kingsway, Edmonton, Alberta - AB T5G 3A6, Canada212 stores
2929 Barnet Highway, Coquitlam, BC V3B 5R5
3401 Dufferin Street, Toronto, Ontario - ON M6A 2T9, Canada188 stores
7275 rue Sherbrooke est, Montreal, QC, H1N 1E9195 stores
3255 Highway 7 East, Markham, ON L3R 3P9199 stores
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text: Home > Business ADB says PH shouldn't rely on BPOs to create jobs ABS-CBN News Posted at May 01 2012 05:44 PM | Updated as of May 02 2012 07:40 AM Share Save Facebook Twitter GPlus LinkedIn MANILA, Philippines - The Asian Development Bank (ADB) is urging the Philippine government not to rely solely on the business process outsourcing industry to create jobs. In the ADB-ANC Development Dialogues aired on Monday evening, ADB managing director Rajat Nag said BPO companies account for only 1% of the total labor force in the Philippines. "We've got to recognize that BPOs provide only 1% of the employment," Nag said. The government needs to have a business plan to revive the manufacturing sector, which will create the jobs the Philippines needs. "The bulk of the growth in employment has to come from industry, has to come from manufacturing. We have to discuss how to make that shift. So this is not an either or. I think the services sector is important, BPO is important, but it will not be enough to create the jobs we need for inclusive growth," Nag said. - ANC Share Save Facebook Share on Twitter GPlus LinkedIn Read More: Asian Development Bank business process outsourcing Rajat Nag /news/05/24/17/duterte-cuts-short-official-visit-to-russia-flies-back-to-ph/video/news/05/24/17/after-marawi-attack-army-insists-ph-still-safe-from-isis/news/05/24/17/obama-did-not-understand-ph-drug-problem-trump-tells-duterte/news/05/24/17/martial-law-in-mindanao-wont-be-abused-army-vows/business/05/24/17/marawi-fighting-stirs-uncertainty-in-financial-markets