text: A Caltorsis protector 79,080pages on this wiki Add New Page Edit VisualEditor History Talk0 Share A Caltorsis protector other resources LootDB · EQ2LL · ZAM EverQuest II Monster Information Race: Skeleton Level: 16 Tier 2 Heroic Zone: Antonica Location: The Ruins of Caltorsis and other spots around the north-east part of the map What does this information mean? Retrieved from "http://eq2.wikia.com/wiki/A_Caltorsis_protector?oldid=613717" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Monsters Skeleton Tier 2 Monsters Tier 2 Heroic Monsters Heroic Monsters Antonica Monsters Monster pages that need EQ2MAP uid Add category Cancel Save


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The Central Naval Command, (CNC) of the Nigerian Navy, Yenagoa, Bayelsa State, on Saturday, said it seized a vessel laden with 600,000 litres of suspected illegally refined automotive gas oil also known as diesel.
This was contained in a statement issued by Lieutenant-Commander Edward Yeibo on behalf of the command's Flag Officer Commanding (FOC).
Yeibo said that two of the four suspected oil thieves were arrested loading the barge with diesel.
He quoted the Commander, Nigerian Navy Ship, Commodore Habib Usman, as saying that a barge and five wooden boats laden with about 600,000 metric tonnes suspected to be illegally refined Automated Gas Oil and two suspects were arrested.
Usman said the suspects were arrested by the Nigerian Navy Ship around Beama community in Nembe Local Government Area in the waterways within Bayelsa State.
The wooden boats were suspected to be used to convey the illegally refined Automated Gas Oil from the refining points to the barge for loading.
"As of the time of the arrest, one of the boats had finished trans-loading its product into the barge.
"Consequently, the personnel of Forward Operating Base FORMOSO with gunboats and tug boat towed the barge to its jetty for further investigation.
"However, the wooden boats were destroyed, the two suspects are in custody for preliminary investigation and prosecution," Yeibo was quoted in the statement.


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Malaria Tropical Parasitic Disease Caused Four Species Protozoan Biology Essay
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This essay has been submitted by a student. This is not an example of the work written by our professional essay writers.
Malaria is a tropical parasitic disease caused by four species of protozoan parasites of the genus Plasmodium, which are Plasmodium falciparum, P.vivax, P. ovalae and P. malariae. In 2004, the simian malaria namely P. knowlesi has been firstly reported in the southern part of Thailand which has been found in Malaysia since 1965 (Jongwutiwes, et al., 2004). Of these species, P. falciparum is the most dangerous form of the disease which can lead to cerebral malaria, coma and death.
Malaria is still one of the world's most important parasitic diseases of developing countries. The WHO | world malaria report 2009 has been reported that half of the world's population is at risk of malaria, and an estimated 243 million people are infected with malaria. It is nearly 863,000 deaths in 2008; almost all deaths occur in African children under 5 years of age (WHO | world malaria report 2009: retrieved October 3rd, 2010). The burden of malaria is mostly found in tropical and sub-tropical areas, throughout sub-Saharan Africa, Southeast Asia, the Pacific Islands, India and Central and South America (Snow et al., 2001).
The epidemiological data of malaria situation in Thailand showed a downward trend in total cases from approximately 200,000 cases in 1991 to some 100,000 cases in 1996. The annual parasite incidence (API) was 2.27 per 1,000 populations in 1999. In 2006, a total of 66,651 malaria cases (both Thai and foreigner) were reported in which Tak, Mae Hong Son and Kanchanaburi provinces are the high incidence of malaria cases ranking respectively first (8,648 cases), third (2,411 cases) and fifth (1,250 cases) of top ten provinces with highest malaria cases reported (Na-Bangchang ; Congpuong, 2007).
The natural infections of the P. falciparum parasite often contain more than one parasite strain. The reason is the human and the mosquito hosts are exposed to heterogeneous parasite populations. Naturally, P. falciparum infections are consist of mixed clones, especially in highly endemic areas (Zakeri et al., 2005; F?rnert et al., 2001).
The merozoite surface protein2 (MSP2) gene has been frequently used as a genetic marker for genotyping of P. falciparum. This gene is divided into two groups; FC27 and 3D7 type as well as by length polymorphisms (Symthe et al., 1990). The length variations of this gene are depended on the repeated sequences, and each allele type can be readily distinguished after gel electrophoresis of Polymerase Chain Reaction (PCR)-amplified products. PCR genotyping for detection of MSP2 has been demonstrate as a great technique to characterize parasite population evenly in small amount of blood samples with low parasitemia (F?rnert et al., 2001).
Another surface antigen gene is the 220-kDa glutamate-rich protein (GLURP) found in all the developmental stages of the parasite, including the surface of newly released merozoites (Stricker et al., 2000). The alleles are classified by size polymorphisms. This gene also has been used as a genetic marker for genotyping of parasite population (F?rnert et al., 2001).
The in vitro cultivation in laboratory was initiated by collecting parasitized blood samples from malaria patient in the endemic areas and then transported these samples by car on the day of collection at ambient temperature. The P. falciparum blood samples were immediately cultured on arrival in order to avoid a loss of some parasite populations in a case of multiple infection. The selective growth of P. falciparum subpopulation in pre-and post-culture samples was observed by Viriyakosol and colleagues (1994) using three surface antigen genes- MSP1 (block 2 and 4), MSP2 and GLURP for parasite characterization. The results showed the pattern alterations of molecular marker which reflected the changing in subpopulations in culture over the time of cultivation.
Nowadays, decreasing in the number of malaria cases in Thailand has been reported. The transported sample by car seems to be high cost consumable method for blood sample collection. This resulted in developing alternative method which more cost effectiveness. Thus, the malaria program, College of Public Health Sciences, conducted the preliminary project to explore the possibility of using EMS as sample transportation. The success of in vitro culture from EMS transported samples was acceptable. Seventy nine percent (34 of 43 samples) from Trat province, 65.8% (52 of 79 samples) from Ranong province, 80.0% (28 of 35 samples) from Tak province, 28.5% (18 of 63 samples) from Ubon Ratchathani province, 71.7% (28 of 39 samples) from Mae Hong Son province and 85.0% (85 of 99 samples) from Kanchanaburi province were successfully grown in vitro. However, the information of the changing or loss of parasite population in pre- and post-cultivation must be further studied. Therefore, this project will be used the two surface antigen genes (MSP2 and GLURP) for examining the changing in P. falciparum subpopulation in pre- and post-cultivation of the EMS-transported samples from three provinces of Thailand.
2.1 Geographic distribution and causative agents
Malaria remains the most important of the tropical diseases, widespread throughout the subtropics and tropics, but also occurring in many temperate zones. The disease exacts a heavy toll of illness and death, especially amongst children in Africa. It also poses a risk to business travelers, tourists and immigrants, and imported cases of malaria are increasingly seen in non-endemic areas such as Europe and North America. The transmission is frequent in rural areas especially in economic developing country. Treatment and control have become more difficult with the spread of P. falciparum resistant to antimalarial drugs, and insecticide resistant strains of the mosquito vectors (WHO, 1990).
The causative agents of human malaria are four species of single-celled protozoan parasites of the genus Plasmodium; P. falciparum, P. vivax, P. ovale, and P. malariae. The geographic distribution of malaria mainly follows the distribution of Anopheles, disease vector. Anopheles can survive and reproduce well in tropical climates. The distribution for each species is showed below:
P. falciparum- throughout tropical Africa, Asia and Latin America
P. vivax- worldwide in tropical and some temperate zones
P. ovale- mainly in tropical West Africa
P. malariae-worldwide but very patchy distribution
In Thailand, the prevalence of the disease remains reported from along the borders between Thailand and Myanmar, Cambodia and Malaysia (Konchom et. al, 2003).
The parasite life cycle involves two hosts. The first one is a vertebrate host (human) and the second one is an invertebrate host (mosquito). During a blood meal, a malaria-infected female Anopheles mosquito with a malaria infection inoculates the sporozoites into the human host. Sporozoites penetrate into the bloodstream then infect liver cells. The sporozoites develop and mature into schizonts (exo-erythrocytic stage), which rupture and release merozoites into blood circulation. This merozoites invade the red cells and develop to mature schizonts in the red cell (erythrocytic stage). The multiple infections normally found in P. falciparum infection. The ring, trophozoites mature into schizonts, which rupture and releasing merozoites again. Some parasites, starting at ring stage differentiate into male and female gametocytes (sexual erythrocytic stage). The blood stage parasites are responsible for the clinical manifestations of the disease.
The sexual cycle starting with, male (microgametocytes) and female (macrogametocytes), are ingested by female Anopheles mosquito during a blood meal. The gametocytes develop and multiply in the mosquito's stomach beginning of the sporogonic cycle. The fertilization is started with the microgametes penetrate into the macrogametes to generate zygotes. The zygotes become motile and elongated in shape called ookinetes which this shape can easily invade the midgut wall of the mosquito where they develop into oocysts. The mature oocysts rupture and release sporozoites which migrate to the mosquito's salivary glands and inoculate into a new human host for starting with new life cycle and malaria transmission happening.
2.3 Genetic diversity of malaria parasites
P. falciparum population in endemic areas is frequently mixed strain or clone, usually containing 1-4 or more clonal populations. The parasite genotypes are identical for each strain or clonal. The mechanisms of genetic recombination, gene conversion, and duplication normally generate new strain in P. falciparum populations. Different parasite strains with different genotype resulting in different phenotypes. This reason effect on the diversity of P. falciparum population in endemic areas. Analysis of the genetic diversity could indicate the structure of parasite population (Snounou G. and Beck H-P., 1998).
P. falciparum displays extensive genetic variation in the merozoite surface antigens, mostly founding in merozoite stage of its life cycle. The marked diversity has been found in genes encoding the merozoite surface protein 1 (MSP1), merozoite surface protein2 (MSP2) and the glutamine rich protein (GLURP). These highly polymorphic genes have been frequently used as genetic markers for genotyping of P. falciparum (Orht et al., 1997).
2.3.1 The merozoite surface protein1 (MSP1 gene)
MSP1 is a major protein on the surface of the blood stages of the parasite. It is composed of a 190-kDa membrane-anchored protein complex, which undergoes proteolytic cleaved into four fragments that remain on the merozoite surface. Before the merozoite (erythrocyte stage) invasion, the entire MSP1 complex is shed, except for the C-terminal 19kDa (MSP119), which remains on the surface as the merozoite enters the erythrocyte (Blackman et al., 1990).
The sequence of MSP1gene can be divided into 17 blocks based on sequence variability. Most of the sequence in MSP1 groups into two distinct allele families (Tanabe et al., 1987), with the exception of block 2, which is repetitive region that consists of four allele families (Takala et al., 2002). Block 17 contains MSP119, which has been the focus of malaria vaccine development because of its highly conserved sequence and hypothesized critical function. (Takala S.L, 2009).
2.3.2 The Merozoite surface protein2 (MSP2) gene
Merozoite surface protein2 (MSP2) gene is a second family of merozoite surface antigens, normally found in the erythrocytic stages. It is a 43-50-kDa glycoprotein and has been localized on the plasma membrane of intracellular and free merozoites. This molecule is anchored on the plasma membrane of the merozoite by a C-terminal glycosylphosphatidylinositol (GPI) moiety (Gerold et al., 1966). MSP2 is highly polymorphic with conserved N- and C-terminal domains flanking a central variable region, which contains tandemly arrayed repetitive sequences. All MSP2 alleles have been categorized into two groups typified by 3D7 and FC27 alleles, respectively, because of differences in the repeats and flanking variable sequences. The MSP2 of P. falciparum displays extreme size and allelic polymorphism.
The MSP2 is divided into five blocks of whole gene sequence. The N- terminal and C-terminal translated sequence, block 1 and block 5, respectively (Figure 3). The block 3 of MSP2 is a polymorphic central region flanked by two non-repetitive regions (block 2 and block 4). The extensive polymorphism of MSP2 alleles is normally used for marked diversity for analysis of P. falciparum population.
2.2.3 The glutamine rich protein (GLURP) gene
The glutamine rich protein is a 220-kDa an exoantigen found in the parasitophorous vacuole of P. fafciparum. The single full-length GLURP sequence (F32 strain) shows two amino acid repeat regions namely R1 and R2 regions (Figure 4). Diversity in GLURP has been indicated by difference sized PCR products the R2 region of various laboratory-adapted and field isolates. The sequence of this gene showed highly conserved among geographically dispersed isolates, and a fusion protein with this gene sequence was able to stimulate B- and T-cells (Borre et al. and Stricker et al.).
2.4 Genotyping of P. falciparum
The most frequently used techniques with interesting potential for identifying genetic variations of P. falciparum are based on the PCR (Mullis et. al, 1986) PCR is a major breakthrough in molecular research because its sensitivity allows the analysis of genes from small amount of parasite materials.
Nested PCR uses two sets of amplification primers (Van et al., 2002). The target DNA sequence of one set of primers (termed 'inner' primers) is located within the target sequence of the second set of primers (termed 'outer' primers).
In practice, a standard PCR reaction is first run with the patient sample using the outer primers. Then a second PCR reaction is run with the inner primers using the product of the first reaction as the amplification target. This procedure increases the sensitivity of the assay by re-amplifying the product of the first reaction in a second reaction. The specificity of the assay is increased because of the inner primers amplify only if the first PCR reaction yielded a specific product. However, nested PCR could not identify different DNA sequences with the same size.
Long and colleagues (1995) were used a standard PCR protocol to amplify P. falciparum DNA which was extracted by methanol extraction from dried blood spot on filter paper. All specimens were collected from participants in a Plasmodium falciparum vaccine trial and from samples collected during a hospital-based study in Thailand. The samples were shipped by mail to the laboratory in Maryland where the assay was carried out. The PCR was performed by amplification of the P. falciparum circumsporozoite protein gene based on a previously published sequence. Sensitivity was 100% when compared with thick blood film results in the vaccine trial (range = 4-60 parasites/?l, median = 8 parasites/ ?l) and 94.6% (range = 3-133,988 parasites/?l, median = 616 parasites) in the hospital study.
Bereczky and colleagues (2005) described a new Tris-EDTA (TE) buffer-based method for extraction of DNA from blood dried on filter paper. The P. falciparum blood samples was blotted onto two types of filter paper; 3MM? filter paper and 903? Schleicher & Schuell filter paper and store for 1-2 year. The DNA extraction was extracted by the three methods; TE buffer-based method, methanol extraction and Chelex? method respectively. The PCR was performed by nested amplification of merozoite surface protein 2 (msp2) which is widely used in molecular epidemiological studies, drug trails to determine the number or types of parasite genotypes of P. falciparum infections. The sensitivity and reproducibility were higher in the TE buffer method than in the Chelex? and methanol method when performed on the two filter paper types stored for 15 and 29 months, respectively. For 903? Schleicher & Schuell filter paper blood spots were lower in sensitivity and reproducibility due to a long storage of these samples. The DNA extraction using TE buffer protocol was done in a few hours prior to the PCR amplification. The effect on long-term storage on the quality of DNA extracted by the TE buffer method still needs to be evaluated.
The research of F?rnert and colleagues (1997) was focused on the observation of the daily dynamics of P. falciparum subpopulations in asymptomatic children in rural Tanzania by using a nested PCR on msp1, msp2 and glurp genes. The P. falciparum subpopulations were observed in the eight children harboring P. falciparum throughout the study period. The parasite populations were found to be highly complex with daily changes in both parasite density and genotyping pattern. These findings implicated that the use of the parasite samples from peripheral blood may be only partly reflect the whole parasite population in an infected individual.
3.1 Sampling
3.1.1 Study site
Blood samples were collected from willing patients who attended for malaria diagnosis at clinics in three provinces (Mae Hong Son, Tak and Kanchanaburi provinces) in Thailand during the year 2007 to 2011.
3.1.2 Sample size
A sample size was calculated as following equation:
Sample size (n) = Z2? PQ/(P- lower limit)2
When, Z? = Confidence level at 95% (Standard value of 1.96)
P = Estimated prevalence of multiple infection in the study area
According to the result of Pumpaibool et al., 2009 found that the prevalence of multiple infected samples from six provinces in Thailand were 44% in Mae Hong Son, 31% in Tak, 27% in Kanchanaburi, 22% in Ubon Ratchathani, 36% in Trat and 32% in Ranong. The selected provinces in this study are Mae Hong Son, Tak and Kanchanaburi provinces with multiple infection from 44%, 31% and 27% respectively. The mean value is equal 34%, so the P value is equal 0.34
Q = 1-p = 1-0.34 = 0.66
The expected multiple infection in this study area is estimated at 0.3 and the lower limit is
estimated at 0.2 then the maximum permissible error (d) is equal 0.1
Sample size (n) = (1.96)2(0.34x0.66)/(0.1)2
= 3.8416x0.2244/0.01
= 86 cases
So the sample will be collected at least 86 cases from three provinces in Thailand during the year 2007 to 2011. The total number of samples both before and after culture are finally equal at least 172 samples for the experiment.
3.1.3 Criteria
Inclusion criteria
- The patient is not less than 5 years old and willing to participate in this research project.
- The patient who has a new infection with P. falciparum diagnosed by a malaria officer.
Exclusion criteria
- The patient is present of one or more of the general danger signs or any sign of severe or complicated malaria.
3.1.4 Ethical considerations
The research proposal will be submitted to Ethical Review Committee for Research Involving Human Research Subjects, Health Science Group, Chulalongkorn University (ECCU) for the ethical clearance of this study project. Informed consent will be obtained from each participant by signature or thumbprint in the presence of a witness. For foreigner participants who do not understand in Thai language both speaking and listening; informed consent will be asked by a malaria official with communicable language.
3.1.5 Parasite collection
The P. falciparum infected patient identified by a malaria official will be taken a small amount of peripheral blood from the selected finger by a malaria official. Before piercing, patient's finger will be cleaned with cotton wool lightly soaked in 70% ethanol for at least two times, allow to dry, puncture the ball of the finger with a sterile lancet. The first drop of blood (about 20-50 ?l) is sucked by using a sterile pipette tip, then put in a micro-centrifuge tube containing 500 ?l of transport medium with 5 units of Heparin, mix by inverting the tube. Another 20-50 of blood is kept in the second tube. The samples are kept in refrigerator (4-10oC). The second drop of blood is absorbed on filter paper, air-dried and put in Zip-locked plastic bag after completely dry. The last drop of blood is used for making the thin and thick film on the same slide. The code number is written on the slide with a soft lead pencil. All specimens are put in a small mail box on the morning of Wednesday and sent by EMS.
The partial samples from the research project under the Malaria program, College of Public Health Sciences were used in this study.
3.2 Microscopic examination and confirmation
Thin and thick blood smears are stained with 10% Giemsa solution for 10-15 minutes. In thin blood film, parasites are counted against 10,000 red blood cells using a magnification of 1,000 X. it can be reported as parasites per 10,000 red blood cells and parasitaemia can be calculated by multiplication of parasite count with red blood cells, then divided by 10,000 (number of red blood cells counted)
Example: Parasite count is 500 per 10,000 red blood cells
Standard red blood cell counted is 4,500,000 cells/?l of blood
Parasitaemia = (500 x 4,500,000)/10,000
= 225,000 parasites/?l of blood
In thick blood films, parasite density can be defined by counting asexual parasites (rings, trophozoites and schizonts) against 200 white blood cells and reported as parasites per 200 white blood cells. The number of parasites relative to leucocytes counted can be calculated and expressed as parasites/?l of blood, from the formula:
(Parasites counted/No. of leukocytes) x 8,000 = parasites/?l of blood
(Karbwang and Harinasuta, 1992)
3.3 In vitro culture of P. falciparum
This study will be used the combined techniques of Trager and Jensen (1976) and Thaithong and colleagues (1994) for culturing of the P. falciparum finger-prick blood samples. The blood sample in transport medium is spun down at 1500 rpm for 2-3 min, the upper layer of transport medium is pipetted off, and the packed cells are transferred to 3 wells of 96-well microtitre plate containing 100 ?l of complete RPMI medium plus 10% pooled serum. The contents of the wells are thoroughly mixed. Then the plates are covered with lids, placed in a candle-jar (Trager and Jensen, 1976), and incubate at 37oC. When the number of parasites has reached 3-5%, the cultures are transferred to Petri dishes for expanding the number and the quantity of parasites.
3.4 DNA extraction protocol
In this study, two DNA extraction protocol will be used: the first one is Chelex? extraction protocol for extraction of genomic DNA of P. falciparum from preserved blood on filter paper as described below:
3.4.1 Chelex? extraction protocol (Bereczky et al., 2005)
One blood spot on filter paper is cut and placed in eppendorf tube and incubated overnight at 4oC in 1 ml of 0.5% saponin in phosphate-buffered saline (PBS). The punches are washed for 30 minutes in PBS at 4oC, then transfered into new tubes containing 25 ?l of stock solution (20% Chelex-100) and 75 ?l of distilled water, and vortexed for 30 seconds. The tubes are heated at 99oC for 15 minutes to elute the DNA, vortexed and centrifuged at 10,000xg for 2 minutes. The supernatant (65 ?l) are transferred into new tubes.
The second one is Phenol-Chloroform extraction protocol will be used for extraction of fresh or frozen P. falciparum blood samples in case of a reference or control case for all PCR reactions. The protocol is described below:
3.4.2 Phenol extraction protocol (Snounou et al., 1993)
Parasite samples are washed twice by 200 ?l ice-cold PBS. 500?l of 0.5% saponin in PBS is mixed to each sample. The mixture is placed at room temperature for 5 minutes or until lysis is observed. The samples are centrifuged at 10,000 rpm for 5 minutes and the supernatant is removed. Pellet is washed 2-3 times with ice-cold PBS and 100 ?l of lysis buffer (Lysis buffer: 40mM Tris-HCl, pH 8.0; 80mM EDTA, pH 8.0; 2% SDS) with 2 mg/ml proteinase K is added. To dilute the mixture, 50 ?l of sterile double distilled water (ddH2O) is added and gently mix. The mixture is incubated overnight at 37oC, at the end of the incubation period; the mixture is adjusted to 30 ?l by adding sterile ddH2O and mix. DNA is extracted with an equal volume of saturated phenol: chloroform (1:1). To precipitate the DNA 1:10 volume of 3 M sodium acetate, pH 5.2 and 2 volume of cold absolute ethanol is added to the extracted solution. After incubation for overnight at -20oC, DNA is pelleted by centrifugation at 13,000 rpm for 10 minutes at 4oC and the supernatant is carefully removed. The pellet is washed with 1 ml cold 70% absolute ethanol and dissolved in 20 ?l of TE buffer (TE buffer: 100mM Tris-HCL, pH 8.0; 10mM EDTA, pH 8.0). One microliter of dissolved DNA is used for PCR amplification.
3.5 PCR amplification for the block 2 of MSP1, block 3 of MSP2 and GLURP sequence
The primers will be used in this research from Snounou et al., 1999 which were designed from published sequences as listed in the UNDP/World Bang/WHO-TDR Malaria Database complied by Ross Coppel. The sequence of the primers and other details in the respective genes are presented in Table 1
The nested PCR amplifications are performed with one microliter of the genomic DNA of P. falciparum, 0.5 units of i-Taq DNA polymerase (Invitrogen), 125 ?M of each dNTPs, 1x PCR buffer, 2 mM MgCl2 and 125 nM of each primer in a total volume of 20 ?l per reaction, in the first reactions. The second amplification is performed in the same condition as in the first amplification, except 1?l of the products are used for the amplification. The reaction mixture is incubated in a Thermocycler (GeneAmp 9700 and Veriti ). The PCR parameter is composed of an initial denaturation period of 5 min at 95oC , annealing for 30 sec at 58oC (the first and nested reactions for GLURP) or 61oC (all nested reactions for msp1 & 2), extension for 30 sec at 72oC, denaturation for 30 sec at 95oC, the last extension for 7 min. The PCR cycles are performed for 20-25 cycles for the outer amplification and 30 cycles for nested amplification. Five microliters of PCR products and 1 ?l of 5x gel loading buffer are analyzed by agarose gel electrophoresis in 1x TBE buffer.
3.6 Visualization of PCR products (Snounou et al., 1993)
Five microliters of 1kb ladder marker and five microlitres of amplification products are mixed with 1 ?l of 5x loading buffer and loaded into each well of 1.5-2% agarose or NuSieve agarose gel. The gel is run at 80 volts for 25-30 minutes. The agarose gel is stained with 0.5 ?g/ml ethibromide for 10-20 min and destained with distilled water for 5-10 min. The stained agarose gel is visualized and photographed with AutoChemi?System by using Labwork 4.0?software (UVP Biomaging Systems) and Cannon Digital camera.
3.7 Data analysis
The estimation for the P. falciparum population in before and after culture of EMS-transported blood samples by the use of PCR technique is generally observed from the multiple bands in the PCR product of PCR amplification which reflect the presence of many different strains in an isolate. The P. falciparum population in all samples will be estimated from the number of bands observed in an agarose gel. For MSP2, the number of FC27 and IC1/3D7 bands is counted.
4.1 Polymorphisms in MSP2 of P. falciparum
The polymorphisms of P. falciparum in each sample were assessed by determining the number and the size variation (alleles) of MSP2 gene. The amplification products form nested PCR of MSP2 as showed by the gel image and table.
Fifteen samples partially used in this study were successfully amplified for MSP2. The number of allele types in each sample analyzed by nested PCR was shown in the table 2. From these observations, it appears that the numbers of allele types of MSP2 ranged from 1 to 2 but it was mostly 1 in the sample after in vitro culture Table 2: The number of allele types analyzed by nested PCR of MSP2 in before and after culture of P. falciparum isolates
Two alleles were detected in four isolates before culture (MH62, MH65, MH67, and T222) but remained one allele type after culture. For MH62, MH65, MH67 isolates were found two bands of FC27 type but mixed FC27 and IC1 type was found in T222 isolate only.
In conclusion, 4/15 (26.66%) samples were changed after culture by disappeared of the band, 1/15 (6.66%) samples was different in size but same allele types.
At the MSP2 loci, 12/15 (80%) samples contained FC27 type and 3/15 (20%) samples contain IC1 type. The predominant allele of MSP2 was FC27 as 12 out of 15 samples (80.0%) harboured FC27 type, 3 out of 15 samples (33.33%) were IC1/3D7 type.
The data presented in this study demonstrate that nested PCR can distinguish FC27 and IC1/3D7 alleles of MSP2 gene. The result showed the changing of allele pattern comparing between before and after culture in MH62, MH65, MH67 and T222 isolates. In T222 isolate disappeared of IC1/3D7 allele in after culture remaining only FC27 allele with smaller size.
This result indicated that the P. falciparum isolates from the endemic areas, transported by EMS to laboratory in Bangkok following in vitro culture were changed in parasite population when using only one MSP2 marker gene. The alterations of P. falciparum population could effect in characteristic of sample using for research experiment.
Viriyakosol and colleagues (1994) used a PCR technique for parasite genotyping with the three surface antigen genes: msp1 block 2 and 4, the whole gene of msp2 and glurp before and after in vitro culture in 101 of a total 269 samples. The changes were detected in one or more of the three markers in the remaining 37 cases. In this research article, the malaria blood samples were collected from all the patients who attended at malarial clinic in the day of collection. The blood samples were taken from venous blood by a doctor and transported to the laboratory in Bangkok on a day of collection and in vitro culture was done as soon as possible on arrival.


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New Student Orientation
The purpose of orientation at Drury University is to provide a variety of fun, interactive experiences for all new students in order to support them in their transition to Drury. Orientation is required of all domestic freshmen and international students, and it is strongly recommended for domestic and international transfer students. The scheduled activities are different for each group. To view the orientation schedules, visit http://www.drury.edu/fye/.
New Students - Fall Semester
Freshmen, transfer and international students attend a four-day orientation event in the days before the first day of classes. In addition to the four-day event, international students are required to attend a two-day program designed for their needs.
New Students - Spring Semester
Freshmen attend a one-day orientation event in January, which is held the Friday before classes begin, as well as the required four-day event in the fall. Transfer students attend the same one-day orientation event in January, and they are strongly encouraged to also attend the four-day fall orientation event. International students are required to attend a four-day orientation event during the spring semester, and they must also attend the four-day orientation event in the fall.


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This short video from Rupert Spira takes us on a three minute journey back to the self. We start as this separate person we call "I" and finish with the realization of our true essence where we have been all along.
In this very short 3 minute video, Rupert explains the transition from Infinite Awareness to the limited self or an apparent limited form of awareness. This is one of those videos that may require multiple viewings to fully appreciate what is being said.
This video clip from Rupert may be only 3 minutes long but it provides a very different perspective on one of the most misunderstood concepts, even by those with decades of study and spiritual practice.
This trailer for Science and Non Duality Anthology (SAND) Volume 1 is offered as an amuse–bouche for the soul. This is a very interesting 3 minute clip of some of the top 21 thinkers of our time from around the world expressing some little tidbits on "Non-Duality".
In this seven minute video clip, Jeff offers wisdom which can help us deal with negative thoughts or thoughts of failure and unworthiness. The mere recognition that thoughts are like sounds which come and go helps defuse their reality so we can resist being drawn in to an emotionally charged story of self pity.
In this six minute video, Neal explores the moral question "Do I have the right to be happy if it will make someone else unhappy?" Let's listen in on what Neal has to say…
In this short five minute audio clip, Neale talks to us about a different way to look at the things in our life we feel are not working which can instantly transform its affect on the inside, as well as eventually transforming its reality on the outside.
In this nine minute video, Rupert responds to a questioner wanting to know how prayer and/or intention fit in to non-duality and if prayer or intention can have any purposeful effect on a desired outcome.
Here is the key to the metaphor that is being spoken of in this video:
Jane's World = Mind and Mary's World = Consciousness (pure Awareness, God)
In this super short four minute video clip from Adyashanti, we take a closer look at our psychological self and what it does in order to keep itself in existence. Without this compulsive activity, the self we take ourselves to be would just cease to exist.
In this super short two minute video moment from Adyashanti, he answers the question "Do we exist without our thoughts?" Let's listen to his answer to this question…
In this 12 minute video, Adyashanti responds to the question "Do I Need a Guru for Awakening?". He explains that no guru can give us "Awakening", but also explains how a "guru" or "teacher" can help one in discovering the truth for ourselves.
Most of us have experienced mind activity that can easily be labeled as "Mind Attacks". This is when the mind brings up a series of thoughts that latch on to our attention and takes it for a trip that seems inescapable. This is often paired with a series of fearful thoughts attaching itself to a deep seated fear. In this 5 minute video, Adyashanti shows us a simple (though not always easy) way to make sure that our sense of identity does not fall into this trap along with the mind.
In this 14 minute video from Rupert, he reminds us of the absolute certainty of Awareness and that in reality there is no such thing as an inside world and an outside world. All appears to exist because of our knowing of it, and yet we do not need this relationship to experience ourselves as we truly are.
This well produced 4 minute video featuring audio clips of wisdom from Alan Watts is focused on the spontaneity of life and how we often stand in the way of our true nature by trying to force life to be what it is not.
In this beautifully produced five minute video, we find Alan Watts sharing wisdom regarding the illusion of memory. Most people use memory to define who they are which is ridiculous since the past is dead and gone. Alan suggests that perhaps if we must define ourselves in some way, we must start with the present.
In this 8 minute video, Rupert offers some wisdom on speaking the truth in every day life and if there is ever any justification for not telling the truth. Let's see what Rupert has to say…
In this 9 minute video from Rupert, he explains that many of us tend to superimpose an expectation, a wrong or right answer, or any other form of projection during self-inquiry, when actually there are endless paths the mind can take on the way to discovering our essential nature. Let's listen in on Rupert as he provides more insight on the process of self-inquiry…
In this wonderful eight minute video from Rupert being translated live in Spanish, he responds to this most common question that can also be expressed as "How does the veiling of Awareness happen?". Rupert uses his common TV screen analogy to brilliantly explain the answer.


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Defector Deux
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|Defector Deux
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Defector Deux may be placed on any two adjacent bunnies in The Bunny Circle. All players roll a die. The highest roller gets the bunnies.
- The player that runs this card may roll twice, but the second roll must be taken, even if lower than the first.
- In case of a tie for the highest roll, the players who tied get one additional roll, with the highest result of those rolls getting the subject bunny.
- Since this is a "colorless" roll, no re-rolls due to pawns are allowed.
- Defector Deux is one of the cards that can affect a Heavenly Halo'd bunny.
- Defector Deux is one of the cards that can affect the Holographic Bunny.


text: Zhane - Hey Mr. D.j (Maxi-CD) 1993 • Genre : Italo Disco HiNrg • Year : 0 • Label : • On demand! (album available 128-320kbs)* #/by GROVER Release code: ODI69460 Added on 27-10-2014, 19:33 You want this album? link doesn't work? report us from your account! Release Info: Zhane - Hey Mr. D.j (Maxi-CD) 1993 Mp3 320 Kbs HQ01. Hey Mr. D.J. (Original Mix)02. Hey Mr. D.J. (7" Remix)03. Hey Mr. D.J. (7" Remix With Rap)04. Hey Mr. D.J. (Maurice's Club Mix)05. Hey Mr. D.J. (Maurice's Remix with Rap)06. Hey Mr. D.J. (Mo's Hey D.J. Work This Dub) This album(s) is currently available for download only with a Premium account. To get a premium account, click here


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Sally Pinkas
browse music by Sally Pinkas published by IMI
Following her London debut at Wigmore Hall, Israeli-born pianist Sally Pinkas has been heard as recitalist and chamber musician throughout the USA, Europe, Asia, Russia and Nigeria. Described by Gramophone Magazine as \"...an artist who melds lucid textures with subtle expressive detailing, minus hints of bombast or mannerism...\", she has appeared with the Boston Pops, Aspen Philharmonia, Jupiter Symphony and the Bulgarian Chamber Orchestra. Her summer credits include festivals at Marlboro, Tanglewood, Aspen, Monadnock, Apple Hill and Rockport, as well as Kfar Blum in Israel, Officina Scotese in Italy and Masters de Pontlevoy in France. Praised for her radiant tone and driving energy, Pinkas commands a wide range of repertoire. With Evan Hirsch (The Hirsch-Pinkas Piano Duo) she has toured extensively, and has premiered and recorded works by George Rochberg, Daniel Pinkham, Peter Child, Kui Dong and Thomas Oboe Lee. With flutist Fenwick Smith she has recorded a 3-CD set featuring the music of Philippe Gaubert for the Naxos label. Other recent collaborations include the Villiers Quartet in London and the Apple Hill Quartet in New Hampshire. She is a member of the Boston-based Trio Tremonti, and of Ensemble Schumann, an Oboe-Viola-Piano Trio. Pinkas\'s solo discography includes works by Schumann, Debussy, Christian Wolff and George Rochberg for the MSR, Naxos, Mode and Centaur labels. Following her critically-acclaimed release of Fauré\'s Nocturnes on Musica Omnia, she has recorded Fauré's Barcarolles and Dolly Suite, as well as his Piano Quartets (both for MSR). The Wall Street Journal praised her "exquisite performance" in her "superlatively well-played" recording of Harold Shapero's Piano Music, recently released on the UK label Toccata Classics. Pinkas holds performance degrees from Indiana University and the New England Conservatory of Music, and a Ph.D. in Composition from Brandeis University. Her principal teachers were Russell Sherman, George Sebok, Luise Vosgerchian and Genia Bar-Niv (piano), Sergiu Natra (composition), and Robert Koff (chamber music). Pianist-in-residence at the Hopkins Center at Dartmouth College, she is Professor of Music at Dartmouth\'s Music Department.


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Crystal Palace co-chairman Steve Parish has explained why he believes Wilfried Zaha is worth £20 million.
The 19-year-old striker has been the subject of increased transfer speculation in recent months and the Palace owners have since put a huge £20 million price tag on him in an attempt to warn off any potential buyers.
Parish reiterated that the starlet is well worth that sort of money and says that the Championship side are not under any pressure to sell.
Parish told Sky Sports: "I think Wilfried is an exceptional talent. I watch him week in, week out. He is obviously young and a little bit special, and I think that that is his value.
"I think he is worth that to a club at least. But that's as much as to set the bar for people so that they know that we're not in a position where we have to sell and they need to come next summer, if that's probably the sort of time we are thinking about, with proper money we expect for talent that the player has got."
Parish also admitted that he is expecting increased interest in Zaha as we approach the January trasnfer window and that clubs may be looking for weaknesses in the player's contract.
"There has been a lot noise and a lot of interest. We have a couple of bids in the past in various transfer windows that we have turned down," he added.
"Right now I think there is a lot of jockying for position, trying to find the weak spots, a clause or some way that they can get him on the cheap. But that's how football works unfortunately."
[post_link url="https://www.footballfancast.com/premiership/tottenham/tottenham-leading-the-race-for-england-starlet,https://www.footballfancast.com/football-blogs/is-he-really-worth-the-transfer-fee,https://www.footballfancast.com/premiership/arsenal/palace-say-zaha-wont-go-for-under-20-million,https://www.footballfancast.com/premiership/arsenal/arsenal-target-not-for-sale,https://www.footballfancast.com/football-blogs/the-20-simply-bizarre-transfers-of-recent-times " target="_blank" type="grid"]


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TAASA Textile Study Group, Sydney
Since its inception in 1994, the Textile Study Group, Sydney has provided members with a regular forum to study and appreciate all aspects of Asian textiles and adornment.
Meetings take place in a relaxed and social environment, where members enjoy presentations from either international & national experts or from within the group, as all are encouraged to research & present their own textile passions.
For more information, contact Marianne Hulsbosch at email@example.com.
TAASA Ceramics Study Group, Sydney
The TAASA Ceramics Study Group, Sydney offers members the opportunity for serious, in depth study of ceramics from all parts of Asia. The group aims to provide a convivial meeting point for collectors, curators and anyone interested in Asian ceramics and its place in the culture of Asia, as well as in contemporary ceramics that follow Asian ceramic traditions.
For more information, contact Margaret White at email@example.com.
Upcoming Events
For Gods, Kings and Ancestors: Ritual Ceramics in Asia
Sat, May 13, 2017
Enjoy a morning exploring the role that ceramics have played in the ceremonial life of various Asian societies, whether in religious or court context.
QLD Textile Interest Group
Collectors, conservators, academics and curators gather three times a year to focus on aspects of the vast and colourful world of Asian textiles.
For more information, contact Mandy Ridley at email@example.com.
Upcoming Events
Contemporary Indian Textiles
Mon, May 1, 2017
On this May Day afternoon tea event, join a discussion about the colourful world of contemporary Indian textiles and hear travellers' tales about textile hunting in India.
QLD Ceramics Interest Group
The Brisbane based Ceramics Interest Group regularly meets in a member's home for a light meal followed by a handling session concentrating on one or more aspects of contemporary and historical Asian ceramics.
For more information, contact James MacKean at firstname.lastname@example.com.
Upcoming Events
Bird and Flower Painting on Asian Ceramics
Tue, Apr 25, 2017
Join us for an ANZAC Day afternoon tea to discuss 'Bird and Flower' motifs on Asian ceramics.


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Miss Yesterday: BackgroundIn 2003, Alan Ayckbourn premiered his play My Sister Sadie. This was one of the playwright's family plays, but marked a notable step forward. The protagonists had become - for the first time - teenagers and the play tackled head-on a number of pertinent of issues that would challenge audiences of any age. Miss Yesterday, his 68th play written in 2004, would continue that trend.
Miss Yesterday contains elements of both My Sister Sadie and his 2000 play, Whenever. The latter dealt with time-travel and the idea that one small event can have immense repercussions (in Whenever's case, the murder of one man leads to mankind annihilating itself in decades of continuous war). My Sister Sadie was an altogether more personal play that dealt with moral issues and how they largely affected one family.
Miss Yesterday merges these ideas in a time-travelling play with very personal consequences; it is a play which concerns family and impact the loss of a sibling can have on it. The focus is very personal and explores how the protagonist, Tammy, deals with the situation. When Tammy is given the opportunity to save her bother she takes the obvious, emotional choice, but her time-travelling actions result in consequences which have unforeseen implications for the entire planet. Tammy is forced to look within herself and re-evaluate her actions not just with regard to how it affects her and her family, but for the rest of the world.
As with My Sister Sadie, the protagonist - Tammy - is a teenager and a complex character with teenage issues such as lack of self-esteem. As Alan has noted, by tackling slightly older characters, his emotional base is widened and, theoretically, sightly older characters can be given much more weighty issues to consider as well as offering a chance to explore the fascinating relationship between parents and their teenage offspring.
Structurally, the play moves backwards and forwards in time and the play is punctuated by Tammy stepping out the narrative to question what she is doing and why. It is not a conventional play, but with an audience that is not necessarily aware of theatrical conventions, the play has a freedom and confidence to play with stage time and the narrative structure. It also makes the audience very aware of the key decisions that affect Tammy's life and why she chooses to make them.
The play opened in December 2004 at the Stephen Joseph Theatre, Scarborough, and was met with mixed but predominantly positive reaction from the press. Again - as had been experienced with previous plays premiered during the Christmas period - the detractors seemed more concerned with the issue as to whether the play was suitable seasonal entertainment rather than on its own merits; although In contrast, other critics praised the play for offering such substantive material at Christmas.
Although the play has not been published, it is available to perform by both professionals and amateurs and has proven to be popular with audiences in the UK and abroad.
Copyright: Simon Murgatroyd. Please do not reproduce without permission of the copyright holder.


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How does a business market itself when it has to keep quiet about its greatest successes? That's the dilemma facing Liz Marks, founder and director of Accomplish, a management coaching practice.
"We have a strong track record providing tailored programmes for business leaders," she explains. "But while we have an impressive client list, we can't publicise it. Nor can we publicise the great projects we've worked on for fear of the clients being identified. When word-of- mouth recommendations are a textbook aspect of marketing success for professional services, how can we get the awareness we need to grow?'
Now in its third year, Accomplish has developed into a family business, with her sister Catherine, an organisational psychologist, recently coming on board. Like most organisations in the sector, it also works with a select group of associates - including some leading writers on coaching - on special assignments.
And some assignments, says Liz Marks, really have been special. "I've worked with people at five out of the top 10 global pharmaceutical companies, for example, as well as quite a number of leaders in investment banks, high-street banks and technology firms."
Recently, she acted as a consultant for the makers of the Channel 4 programme Boss Swap.
She first became interested in coaching when she was a small business adviser. "I was working with a lot of owner-managers who were running promising firms, and I realised that what they really got value from was coaching. Whether I was giving them feedback on how they seemed to be managing, advising them to come across in a different way or just acting as a sounding board, they really benefited from it.
"I came to realise, as they did, that their personality, management style and approach to life could dictate whether their business grew or failed. It was one of those lightbulb moments."
New business leaders reap the most rewards from coaching, she has since discovered. "Many are accomplished technically, but once they are expected to manage or lead, they find they need something more."
After spending three years establishing Accomplish and depending on referrals and networking to get more business, Ms Marks admits she hasn't had time to do much marketing. "I'd now like to use it to help us grow to the next stage."
Elizabeth Ferguson, immediate past president, International Coach Federation (UK)
"Accomplish is right to be concerned about confidentiality: it is at the heart of any coaching agreement. But we have found that big clients are happy to provide testimonials when they feel they have benefited. Often, the challenge is having the courage to ask.
"If testimonials cannot 'name' the specific client, they can refer to market sectors and seniority within the business. Accomplish can also use this anonymity to its advantage, by emphasising its confidentiality standards."
Alan Brooks, director, Hyde Marketing
"Marketing highly sensitive business recovery services for PricewaterhouseCoopers highlighted to me that professionals can build reputations without breaching client confidentiality.
"Accomplish can still exploit its strong track record by recruiting former clients as advocates. Regular one-to-one communication - not mass mailshots - will help maintain relationships even after assignments have finished, keeping it 'top of mind'.
"With a customer base among large organisations, there is real scope for growth. While not 'going public', clients may still make recommendations to close colleagues or human resources professionals. To make this work, Accomplish must have materials that reflect its strong vision and inspire enough confidence among clients for the word to be passed on.
"Having articles published, offering media comment on general trends, and getting speaking appointments will all help build profile and trust and generate leads."
Laurie Young, international board trustee, the Chartered Institute of Marketing
"Ms Marks' confidentiality restrictions are shared by other professionals but need not be a constraint on business growth.
"If the firm's reputation is good, it will receive repeat business and referrals. This will keep her sales costs low and prices high. Marketing must enhance this virtuous circle.
"First, step back and find out what the reputation is, and then find ways to amplify it. Join networking organisations that clients attend, and develop relationships with intermediaries that they respect. Hire a freelance public relations expert to get you on to conference platforms and in magazines. Have a website that clients can access. Finally, ask satisfied clients to become referees.
"Ms Marks must decide her business focus: large corporates, owner managers, industry specialisation or new offerings. Most professional start-ups take up all leads in their early years. However, those that grow tend to focus on a few key clients."
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Town Square
Foothill High plant sale today to benefit Special Ed program
Original post made on Mar 27, 2013
Read the full story here Web Link posted Wednesday, March 27, 2013, 7:41 AM
Comments (1)
a resident of Foothill High School
on Mar 27, 2013 at 9:34 am
I bought a mix of herbs and vegetable plants from Gail's kids last year. They were much nicer than plants found in nurseries and the variety of plants, including heirloom varieties was excellent. Great prices, too!
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Valley Trails site is poor location for school
By Tim Hunt | 7 comments | 668 views
Nominations due Monday for TV30's Tri-Valley 'Coach of the Year' award
By Jeb Bing | 0 comments | 95 views
Readers' Choice Ballot is here
It's time to decide what local business is worthy of the title "Pleasanton Readers' Choice" — and you get to decide! Cast your ballot online. Voting ends May 21st. Stay tuned for the results in the June 23rd issue of the Pleasanton Weekly.


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Toowoomba, Queensland, Australia 1903 – Sydney, New South Wales, Australia 1976
22 March, 1903 – 14 December, 1976
not titled [Bare trees].
Collection Title: Sketchbook of scenes, people, living things, and steel production at BHP, Newcastle
Page: Leaf 14 recto.
Place made: New South Wales, Australia
Materials & Technique: drawings, sketchbooks, graphite; paper; pastel drawing in black pencil and pastel Support: paper


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April Special Offer! Add something special to your project this month with 10% off our entire Effect Fibre range! - Find out more
Greasy Raw White Jacob Fleecewool
- Code:
- Weight:
- 100g
Straight from the Sheep! This is perfect for those who want to create their masterpiece from scratch. Please note that this greasy fleece may have a whiff of nature and have a little matter remaining, but don't worry, we do sell a scouring agent for you to get going!
Jacob wool is a medium quality, natural coloured wool. It is the classic hand spinners wool. Our tops are made from selected Jacob fleeces. 33-35 Micron, average length of 85-95mm Perfect for hand spinning, hand felting and many other craft uses.
Microns: 33-35mic
Fibre Length: 85-95mm
(All measurements are approximate)
Please Note: Colours may vary slightly from batch to batch due to the nature of natural fibre. We have taken every step to represent the colours correctly, but we do recommend ordering a sample for clarity as colours can change depending on your screen settings.
If ordering tops, roving or jumbo yarn, all quantities will be sent in a continual length (up to 10kg) unless otherwise stated or requested.


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My remarkable grandson asked the following, "papa, when you pee, can you stop and then start again?" Thank god he didn't ask if worms can dream; he did cause me however to stop and marvel about what comes into the mind of a three year old.
The question also brought memories of things that little boys do that are so easily forgotten by grizzled men, like me.
There was a time you see when I kept my eyes mainly on the ground when I walked. Most every week Henry Hagarty's lad would find loose change lying not only outside of any place of business but also most anywhere…in the grass, on the sidewalk, under a rug or on the road. At that time pennies were not derisively shunned as they are now. A mere penny could get a young hellion three fat jube jubes. Combine a lowly penny with an empty pop bottle or two, and you could get a small bag of glorious bonbons. And candy, dear friends was of utmost importance to a wee tadpole.
Spitting was also a shared pleasure of boys. Spitting the farthest was a matter of great importance to all… and hawking loogies brought many accolades from pals, if said loogie was spit far enough and bounced multiple times before it settled into a serene puddle. Colour mattered too; example…conjuring one up, after the downing of a black licorice pipe, made a darkly triumphant splash to little boy culture…as interfering and reprimanding little girls gagged with gusto.
Peeing contests gave mere boys great satisfaction, it mattered who could pee the farthest, for the greatest duration and to simply make the best noisiest backwash against any wall or tree….an impressive arc also brought kudos from friends. I swear that once my arc was so prolific that a rainbow edged its way into my stream.
Underwear rust contests also gave us great enjoyment when my pals and I were in our salad days. I can recall changing at Barbers Beach with Roy and Red and trying to determine who has the greatest brown streak on their pristine white jockeys. Note, in those times underwear was white only. Anyway, I am proud to tell the reader that the winner was the one with the nastiest brown streak. He would be jokingly derided and jeered…but with great respect and envy.
Soon, Keenan will be attempting to whistle and with much practice he'll be able to carry a tune of a favourite song, or call a friend via a secret code with his newfound vocal toy.
Sadly, in that important art, I could never whistle in the coolest way, which was to put both of your index fingers under the tongue and rip out a fearsome trill. I still envy those males who can whistle in that illusive manner.
Boys of my generation also spent a great deal of time relaxing and looking up to the heavens. Clouds and cloud shapes were important to us at the time…and how fast they moved, peaked our curiosity.
Climbing was mandatory. It didn't matter whether we were hefting ourselves up to sneak a cookie in a Himalayan cupboard, climbing a tree or mounting a pal's back in order to have giant fights…climbing was of biological and sociological necessity for all young males.
Anyway, no time to talk about Keenan's future discoveries like; looking at contrails, not stepping on cracks, torturing bugs, tormenting girls, star gazing or asking mom and dad a thousand times a day, questions that started with the inquisitive 'why' word.
I'll end this now and tell the reader what I told Keenan when he asked if I could stop peeing in midstream.
My answer was 'yes, my remarkable boy, papa can stop but chooses not to.'
I think at times that I'd like to go back for even a day as a young pollywog. Wouldn't it be great to view the world from the eyes of a young boy?…to see the possibilities and mystery of all that surround us.


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- Easter Week
- ¨ Holy Thursday 4/13 @ 6:30
- Sunday - 9:00am
- Sunday - 11:00am
Las Vegas , NV 89130
(702) 910-3350 - Parish Office
(702) 429-3478 - Emergencies
fr Pete Seebold - email@example.com
Bible Study
Achieving the desired success requires patience and persistence. Your goals need time. You must study, improve yourself, go towards your target with patience. With a deeper thinking you'll see the importance of your life.
Women's Bible Study
Janet Burgess is offering a Women's Bible Study Class. Starting August 4, 2016 - 1st and 3rd Thursday's @ 1:00pm. Contact Janet Burgess or Cathy May for questions or suggestions
Mass and Order of St Luke Healing Service
Mass and Healing will be on Wednesday January 18, 2017
Our Wednesday night Mass and OSL Healing Service was held July 20. It was very well accepted and people really complimented on it. We had 32 people attend. Due the interest shown we will hold this Mass and Service the 3rd Wednesday of the month at 6 pm mass and 6:30 pm healing service. If you missed last month come and see what its all about. This is truly a blessing from God for St. Barnabas.
Help and Hope for Kids
This our Church Charity
Hi I'm Stanley, my wife CeCe and I are the founders of Help And Hope For Kids, a 501(c)3 non-profit charity. We are supporting a village in the town Ara-al, Philippines. This town is located high in the mountains miles from La Carlota City, Philippines. The families there live a hard life*. Children start working at a very young age because most families make very little money per pay check. The chance to express their childhood is taken from them way too early.
upcoming events:
Pray the Rosary
They experienced the power. They saw the results. They were incredibly impressed
Fast-forward two thousand years! Much of what passes as prayer are memorized words, faith-less repetition and a lot of whining, No inspiration here.
Bible Study
If this is totally new to you, no worry. A number of good sites on-line can instruct you.
But for starters, try this. Take one verse of Scripture that has meaning for you and that you have need of. Try Psalm 91:11. He sets His angels to guard you in all your ways.
Sun - 1-1-17
- To The Congregation of St. Barnabas
December 07, 2016 - 7 PM Wednesday
Olive Garden - 6850 W. Cheyenne Ave. - Las Vegas, NV 89108
Please sign up for accurate count to give the RESTURANT FOR SEATING.
I'm New Here
Slowly repeat that verse several times. Let it roll around on the inside. Consider the meaning of it. Now reconstruct the verse to be personal and present tense. Lord, thank you. You have set your angels to guard me in all my ways.read more
audio - video podcasts - Written Homilies
Can you feel the verse begin to root in you? Strengthen you? Fill you with some confidence? Give this activity some attention in moments of quiet. You will probably have to make the quiet.go to videos
prayer request
Well worth the effort! Find another verse in Psalm 91 and repeat the process. Can you feel the difference between this type of prayer and those prayers you have found so frustrating?read more
care ministries
To speed up your new prayer training and to ramp up the power of it, pray with two or three others. Each of you take time to pray aloud while the other two enter in silently. When a small group prays Scripture ...read more


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Re: Retaining Wall Surcharge in Snow Country[Subject Prev][Subject Next][Thread Prev][Thread Next]
- To: <seaint(--nospam--at)seaint.org>
- Subject: Re: Retaining Wall Surcharge in Snow Country
- From: "Randy Vogelgesang" <rvogel(--nospam--at)jps.net>
- Date: Wed, 9 Jun 1999 21:50:38 -0700
Tom, I posted this exact same topic about a year ago and did not receive much of a response. One thoughtful engineer suggested that frozen soil has no active pressure and as a result negates any surcharge. Good point however our frost line is only 18+ACI-. I personally haven't seen any negative effects of walls that where this surcharge is not considered (even really old walls). One possibility is that full active pressure takes along time to fully develop and that the FULL ground snow is very seldom on the ground (if ever). Currently the EL Dorado County Building Dept. is looking at this subject. I understand that they haven't have had very much input. My personal opinion is that the retaining walls seem unaffected by ground snow load and that money required to beef up retaining walls might be better spent on other parts of the structure. I will be looking forward to input on this subject and will forward any relevant info to the EDC building dept. Randy Vogelgesang S.E. South Lake Tahoe -----Original Message----- From: Tom Schaniel +ADw-tschaniel+AEA-QNET.COM+AD4- To: seaint+AEA-seaint.org +ADw-seaint+AEA-seaint.org+AD4- Date: Wednesday, June 09, 1999 6:47 PM Subject: Retaining Wall Surcharge in Snow Country +AD4-Does anyone out there who does snow country design have any opinions on what +AD4-to use for a surcharge on retaining walls. Would you use the ground snow +AD4-weight, or some other number. For example we have tables that say to use +AD4-100 psf surcharge for garages and parking areas, and 200 psf surcharges for +AD4-highways. In areas with a 300 psf ground snow weight it seems out of line +AD4-to be using this number directly and getting tougher retaining walls then +AD4-would be used at a highway. We'd appreciate any thoughts or recommended +AD4-references. +AD4- +AD4-Tom Schaniel +AD4-BILL JENKINS ASSOCIATES +AD4-Mammoth Lakes, CA
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text:
Qiaobi detergent promises to fight stains and wash away any faith in humanity you might be feeling at the moment.
"Om nom nom! Delicious little drivers!"
Are you green with envy or just plain nausea?
These two men in China show us that no obstacle is a match for the bond of friendship.
It's no secret that many countries face looming demographics problems as their populations age.
Much has been written about developed markets like Japan, Germany, and Italy, which have the largest percentages of their populations age 65 and up.
But another country also deserves attention: China.
A roly-poly bear with red cheeks and white eyebrows recently appeared on a TV program in China, which can mean only one thing: Kumamoto Prefecture traveled six years back in time and ripped them off!
Eh, beats walking/swimming, right?
Artist digitally manipulates videos of kung fu masters practicing their craft to create sequences of mesmerizing motion.
You know it's high art when you can't tell if zero effort or a ton of effort went into making it.
Just one quick question: Can we keep it?
And yes, it comes in both original and hot and spicy flavors.
These days, the "sincerest form of flattery" is bound to land you in legal hot water.
When your manliness cannot be contained by boxers intended for mere mortals.
It's big, it's bold, it's brash, and it's going to China.
If you ever wondered what unicorns eat, now you have your answer.
There's so much more to China's southern city than skyscrapers…
Follow-up interviews to the dramatic Marriage Market Takeover documentary shed light on the beliefs of China's "Leftover Women" and their parents' eroding difficulties in accepting them.
It's not very often we're able to follow the line "he never even saw it coming" with "but then he casually watched it zoom over the horizon…"
Forming a promotional flash mob in China's Sichuan Province, these ladies impressed not with singing and dancing, but with their graceful silhouettes.


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Home Etc #95
Hello! A warm welcome back to our lovely home & interiors linky, Home Etc. We love our own homes & gardens so much and we also thoroughly enjoy reading all the superb posts that link up each week…so thank you! You give us such inspiration. Our **favourites posts** from last week were from:View Post


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Doctor insights on:
Treat Dark Lip Hair
What can I do to treat my dark upper lip hair?
Many options: Some people may use a topical bleaching agent to lighten the hair. Various hair removal methods may be your best options to completely rid yourself of this. Seeing a dermatologist will help you determine what is the cause and what treatment may be best for you. ...Read moreSee 1 more doctor answer
Why is the hair above my upper lip suddenly darker?
Sure it is your hair: Make sure it is the hair and not the skin. I bet it is this skin and you have something called melasma.If so, this can be treated with the use of the chain creams and the constant use of sunscreen. Don't forget a broad-brimmed hat in the summer. ...Read moreSee 1 more doctor answer
What can cause top of the vagina where hairs start to itch and the lips? How is it treated?
Several things: Vaginal itching without discharge can indicate infections like yeast or stis like herpes. It can indicate an allergic reaction to household products like certain soaps and laundry detergents. There are also some skin conditions liked lichen sclerosis which can cause vaginal itching. Hormone deficiencies from menopause or breast feeding can also cause itching. ...Read more
I have some unwanted hair on my upper lip and I was wondering if I were to get it waxed if it would grow back in darker or more thick?
No: You can wax or shave or pluck or laser. None of these will change the type of hairs or the growth. ...Read more
Can ihave a name of a cream not what is added in it,want to know an actually name of cream that remove upper lip dark shadow it's my skin not hair!!
Yes : There is a cream called hydroquinone that can help with facial discoloration. It is a prescription cream and may or may not be covered by insurance. It is not guaranteed to work and may take a long time but you could ask your doctor about it. Hope that helps!! ...Read more
Is it safe to take out upper lip hair using tweezers? Will it grow back thicker or darker in colour?
Yes and No: Yes it is safe to tweeze hair out, although it is painful. Hair will grow at a fixed rate whether it is plucked or not, so you won't alter the hair by tweezing it. ...Read more
15 years old with dark patch above upper lip and below nostrils -but its not hair! any solutions? Is it melesma?
Dark patch: The dark patch above your upper lip may be a nevus laser treatment may be needed. ...Read more
I need to wax my upper lip (light blonde hairs) but I am afraid the hairs will start growing back dark and coarse. Is this true?
Waxing: No. This is an Old wives' tale. ...Read moreSee 1 more doctor answer
Sugaring vs regular wax? Is it ok to use sugaring on face and would facial hair grow back darker? Never removed full facial hair only upper lip
No: never heard external sugar darkening hair growth after ...Read more
- Talk to a doctor live online for free
- How to treat dark upper lip?
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- Why is the hair above my upper lip suddenly darker?
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text: Inspire, Innovate ... Transform The Wisconsin Technical College System (WTCS) Whether gauging the value employers place on our role in the success of their business, or measuring student success and satisfaction... Wisconsin's technical colleges deliver. Message from Dr. Morna K. Foy, System President Latest News View All News Students Named Tech College Ambassadors WTCS recognized16 technical college students as "State Ambassadors" at a ceremony in Madison Wednesday evening. More about post: Students Named Tech College Ambassadors Great Lakes awards $1.3 Million Student Success Center grant to WTCS A $1.3 million, three-year grant from Great Lakes Higher Education Corporation & Affiliates will allow Wisconsin to become the 14th state to implement a "Student Success Center" More about post: Great Lakes awards $1.3 Million Student Success Center grant to WTCS Phillips-Medisize named WTCS Futuremaker Partner WTCS and Chippewa Valley Technical College (CVTC) honored Phillips-Medisize Corporation Tuesday evening as a WTCS Futuremaker Partner for its commitment to technical college education. More about post: Phillips-Medisize named WTCS Futuremaker Partner


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French toast for all my friends!
Posted on May 13th, 2013
y husband hates making French toast.
This wasn't always the case. There was a time, as recently as a year or so ago, when making French toast was an event to which we all looked forward. A local bakery near the preschool the Three attended sold a loaf of cinnamon raisin bread that Scott would transform into French toast heaven. Sometime late in the week before a French toast event, I would begin buying up berries at the store.
Occasionally, we would even have homemade whipped cream.
But the children got older. And hungrier. French toast weekends became less about process (ooh, powdered sugar! ooh, cinnamon! bananas, please!) and more about product—specifically, how much could be produced in the shortest amount of time. Upon receiving a plate of sourdough French toast garnished with raspberries, Roy will clean the fruit off the top first as if he's heard there will be ribbons handed out at the end of breakfast for speed. He moves onto his two meager slices of syrup-infused bread only when he's completed the stupid, nutritional compulsories.
"That was delicious, Dad!" he shouts at the chef from 18 inches away, his plate in hand as clean as if we'd let our dog have at it. "Can I have another piece?"
Then, once more, just to clarify: "A whole piece?"
And then: "Is Peaches done with hers?"
You can understand how making French toast has become the strangest of hybrids: high-pressure drudge work, probably not entirely dissimilar from what Lucy and Ethel did on their confectionary assembly line only without the chocolate.
The nadir of the experience is that we never quite manage to have enough for Scott to have his own French toast to eat. I'm not sure if it's that I actually believe that the half-loaf of sourdough we have living on the counter is sufficient to feed us all come the weekend or if I'm just too cheap to buy another full loaf of the good bread to make absolutely sure that we're covered. All I can tell you is that by the time the rest of us are finishing our French toast, Scott is making one piece for himself out of the butt end of last week's whole wheat sandwich bread. Sometimes he would burn it. It was on these mornings more than any other that the rest of us—our bellies full, our spirits high—would remark at Daddy's inexplicably terrible mood until, say, lunch.
So we've been off French toast for a while. It's been dead to us. But time truly does heal all wounds and the magic combination of six months off, a partial loaf of sourdough going stale and the quiet threat that is Mother's Day translated into French toast for four.
(We forgot to call Marcel in for breakfast. Stop looking at me like that. My hand to God, we just forgot!)∗


text:
Sector-42, Chandigarh, India- 160036.
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Sector-42, Chandigarh, India- 160036. .


text:
AllProtek Universal Surface Protection
Outstanding adhesive strength for both horizontal and vertical applications.
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This film has been doing a great job for decades! What makes ours great?
- Made in the USA
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Surface protection for counters
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- Clear and Blue tinted film, 3 mil thickness
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text:
Online 8 days ago
Rent budget
Preferred stay length
Move date
Hi my name is Charlie.
Im a 25yo professional working in Queanbeyan.
My situation is I need a place to stay until a place I bought off the plan is built.
Im very easy going, and away for work a fair bit.
Please let me know If there is a time that would suit for me to say hi and check your place out! :)
Charlie is also interested in Teaming-Up and starting a new share house.


text:
Actions and Detail Panel
Daisy Pulls it Off - Friday
Fri 19 May 2017, 19:30 – 22:00 BST
Friday 19th May
'53 Drama Group presents
Denise Deegan's Olivier award winning comedy Daisy Pulls it Off. Set in the 1920s, this parody of the jolly hockey-sticks girls' boarding school world of Angela Brazil, with its dorm feasts, hidden treasure and climactic cliff-edge rescue, has more than a hint of Harry Potter, though with more laughs - not least because the girls are played by adults!
Daisy is the first elementary school pupil to win a scholarship to an exclusive girls' private school, and the play is about the obstacles and prejudices she has to overcome. Taken as an escapist romp, or as a comment on snobbishness and divisions in English society, it offers apt entertainment for our times.
Tickets £7 Concessions £5
Doors 6.45pm Curtain up 7.30pm


text:
Another Regional Access exclusive from Emmy's! Only $22 per case from now until they are gone.
We took our signature macaroon and hand dipped it in 84% dark chocolate that's been lightly sweetened with organic coconut sugar. This is one of our best tasting confections to date! Why'd we do it?? Because everything is better dipped in chocolate.
Code # EO525 Macaroons, Chocolate Dipped Coconut Vanilla 12/2oz
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Jesse Wysong
Communications Director at Regional Access, Inc.
Communications Director, database hound, order enter-er, IT department, occasional warehouse worker, support specialist, and general systems guy at Regional Access.
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- Maple Hill Grass-fed Milk Now Available - April 3, 2017
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Member Favorites: 0
Nene Kinokuni (紀ノ国 寧々)She is a 91st Generation student of Tootsuki Culinary Academy and the current 6th seat of the Elite Ten Council.
Nene is extremely taciturn, speaking very bluntly if she even speaks at all. Her personality directly conflicts with Terunori Kuga, who is much more loud and brash, often leading to her to vocally tell him to die or jabbing at him using his height.
Voice ActorsNo voice actors have been added to this character. Help improve our database by searching for a voice actor, and adding this character to their roles here.


text:
This sounds good: "'Cognitive Capitalism' and the Rat-Race: How Capital Measures Immaterial Labour in British Universities" by Massimo de Angelis and David Harvie
One hundred years ago, Frederick Taylor and the pioneers of scientific management went into battle on US factory-floors. Armed with stopwatches and clipboards, they were fighting a war over measure. A century on and capitalist production has spread far beyond the factory walls and the confines of 'national economies'. Although capitalism increasingly seems to rely on 'cognitive' and 'immaterial' forms of labour and social cooperation, the war over measure continues. Armies of economists, statisticians, management-scientists, information-specialists, accountants and others are engaged in a struggle to connect heterogeneous concrete human activities on the basis of equal quantities of human labour in the abstract – that is, to link work and capitalist value. In this paper, we discuss contemporary capital's attempt to (re)impose the 'law of value' through its measuring of immaterial labour. Using the example of higher education in the UK – a 'frontline' of capitalist development – as our case-study, we explain how measuring takes places on various 'self-similar' levels of social organisation. We suggest that such processes are both diachronic and synchronic: socially-necessary labour-times of 'immaterial doings' are emerging and being driven down at the same time as heterogeneous concrete activities are being made commensurable. Alongside more overt attacks on academic freedom, it is in this way that neoliberalism appears on campus.
available from here (subscription or Athens login needed)


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29 August 2013
I was wondering about the access codes for the Lennon Online Universe included in the box sets. It seems the website is no longer up - is any of the content for it available elsewhere? If not, did I miss anything special?
As an aside, this is the sort of transience which turns me off a lot of electronic content.
==> trcanberra and hongkonglady - Together even when not (married for those not in the know!) <==
18 March 2013
I bought a JL greatest hits CD over the summer because it came with his music videos. It said there was a code inside but it expired in mid-2012! I have no clue what was on it and to be honest I don't really care but that's a stupid idea, putting codes for bonus features and then having them expire a year after the CD was released O.o 'tis thick!
INTROVERTS UNITE! Separately....in your own homes!
Make Love, Not Wardrobes!
"Stop throwing jelly beans at me"- George Harrison
18 April 2013
I got that John Lennon box set in the mail ONE DAY after the access code expired.
"Remember, we are writing in the sky instead of on paper--that's our song. Lift your eyes and look up in the sky. There's our message. Lift your eyes again and look around you, and you will see that you are walking in the sky, which extends to the ground. We are all part of the sky, more so than of the ground. Remember, we love you." --John Lennon and Yoko Ono
20 August 2013
Expert Textpert said
I got that John Lennon box set in the mail ONE DAY after the access code expired.
Ouch! That stings!
Can buy Joe love! Amazon | iTunes
Check here for "how do I do this" guide to the forum. (2016)
I forgot about that. I bought the box when it came out but I don't know if I ever looked at the website. I did the same with Paul's Kisses On The Bottom - completely ignored the card that offered a live download. Might see if that still works.
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He didn't use to. Recently, he will go to the door and act like he wants out. I go to open the door he runs off. I pick him up to put him outside he pees on me. Why? When he is outside he spends the whole time scratching on the door and I find puddles of pee in the house. What is wrong with him?
Answer by SuperChicken at 11:47 AM on Oct. 15, 2010
Answer by kiansmom0423 at 11:45 AM on Oct. 15, 2010
Answer by dancer at 12:00 PM on Oct. 15, 2010
Answer by Nanixh at 12:11 PM on Oct. 15, 2010
Answer by mompam at 11:49 AM on Oct. 15, 2010
I agree with Super Chicken it could be a bladder infection also, he may have had a problem outside and is acting it out this way. Good luck and I hope your furbies gets better with this.
Answer by zbee at 3:48 PM on Oct. 15, 2010
Answer by luvmydoggies at 11:02 PM on Oct. 15, 2010
Answer by kileighsmommie at 1:18 AM on Oct. 16, 2010
Answer by _Tam_ at 12:41 PM on Oct. 28, 2010
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text: Content is king. That's what we used to say 10 years ago. And it's still king – sort of. The issue is that as more and more people vie for visibility and authority, the same stories get repackaged in a different wrapper and published over and over. And the overall value of the content diminishes over time.


text:
So I recently purchased a few of the Gosh Velvet Touch waterproof eyeliners (€7.99) and i must say, theyre probably the best drugstore eyeliners you can get. Super-soft, highly pigmented, easily blended and long lasting. They make a great eyeshadow base and the variety of colours is amazing. Heres the ones that I got:
Black Ink
Deep Black Matte Colour, Blends beautyfully, waterproofness: Fades from waterline in about 2 hours. 9/10
Metallic Brass
Dark Brown shot through with Gold particles, very pretty. Blends lovely, nice alternative to wear on the waterline. Stays on slightly better than Black Ink. 9/10
Sparkling Pearl (Crystal Eyes)
Gorgeous Creamy Colour with loads of fine sparkle. What should have been a more flattering alternative to white liner, unfortunately you cant see this colour on your waterline at all, because you can ony see it when it catches the light. Same qualities as the other two, so I now use this to highlightaround the tearduct, under the brow and as a base for lighter toned eyeshadows. Very disappointed about the waterline thing though. 8/10
Overall, I definitely recommend these liners, amazing value. Another thing i like about these is that when you sharpen them the point remains nice and rounded, instead of being so sharp it could pierce your lid. GOSH is fast becoming my favourite high street make-up brand. Superdrug sells GOSH and well stocked local pharmacies should have it too :) Love xxx


text:
Parking prices may go up
Sun., June 17, 2007, midnight
The Spokane City Council soon will consider making downtown parking more expensive – partly to help pay off bonds for the River Park Square parking garage.
A proposal endorsed by Mayor Dennis Hession would increase parking meter rates, enforcement hours and parking tickets.
Most street parking prices would rise 25 percent. Meters would have to be plugged 9 a.m. to 7 p.m. – one hour later than current hours. The plan is expected to be considered by the council next month.
Parking charges have not changed since 2000 and prices are below market rate, said Dave Steele, the city's acting real estate manager who worked on the proposal.
The changes would raise an extra $500,000 to $700,000 a year. That figure includes $110,000 expected from writing more tickets as a result of increasing the city's meter enforcement crew from six to seven.
"Our plan is to raise parking meter rates to current market levels and use the proceeds to fund some critical needs in a partnership with the downtown business community," Hession said in a written statement.
Most parking meter money goes to make bond payments the city owes as a result of its settlement with the Cowles Company over the River Park Square parking garage. That settlement severed the formerly public-private ownership of the garage after years of legal wrangling. The Cowles Company also owns The Spokesman-Review.
The new rates were recommended earlier this year by the Matrix Consulting Group in an efficiency study the California firm wrote for the city.
For the last few years the city has collected about $1.8 million annually from parking meters. On top of that, the city brings in about $1.1 million from parking tickets.
Next year bond payments for the garage will increase from $1.8 million to $2.2 million.
Spokane Budget Director Tim Dunivant said the city banked money a couple years back in anticipation of the higher payments, but current meter rates can sustain bond payments only through 2013, he said.
Hession supports using $100,000 of the new money to pay half the cost of an update to a downtown development plan first completed in 2000. The Downtown Spokane Partnership has raised the other half.
Marty Dickinson, president of the partnership, said the plan will examine issues like bike transportation, building height rules and converting one-way streets to two-way.
"We believe that if they're going to raise the rates, then a portion of the funds should come back into downtown," Dickinson said.
She added that an update is needed because the city's core has changed rapidly and many of the goals of the 2000 version have been achieved.
The parking proposal also would give the downtown group 8 percent of revenue above what was raised in 2006 to use for a downtown cleanup team, security and other programs.
Money left after bond payments and downtown spending likely would be used for street maintenance – where most meter money used to go before the River Park Square controversy arose.
Dickinson said many businesses would support heavier parking meter enforcement to cut down on office employees who continually plug meters during the day.
"We want the parking meters to be used for patrons," Dickinson said. "It would help to discourage those who are abusing the meters."
Councilman Bob Apple said he's leaning against the increases.
"If I were to hear that support I would be more inclined, but I need to hear that from the public," he said.


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Maxwell Chrysler Dodge Jeep, Automotive Repair, Automotive Sales & Services, listed under "Automotive Repair, Automotive Sales & Services" category, is located at 14150 State Highway 79 Taylor TX, 76574 and can be reached by 8556426997 phone number. Maxwell Chrysler Dodge Jeep has currently 0 reviews.This business profile is not yet claimed, and if you are the owner, claim your business profile for free. If you are not the owner you can invite this business to join by email or by phone.Browse all Automotive Repair, Automotive Sales & Services in Taylor TX. Discover census data for Taylor, TX.
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