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Tawanna's Children's Short Story Collections
Written by Tawanna Bumpus
Illustrated by Elayna Jenkins
Lovereading4kids Price £11.95In Stock
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Tawanna's Children's Short Story Collections by Tawanna Bumpus
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48 pages
Tawanna Bumpus
More books by Tawanna Bumpus
Outskirts Press
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subset: commoncrawl


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Published byMarc Gadsden Modified over 2 years ago
From Baghdad to Beirut: Iraqi Refugees' Voices for Equity in Transit
Jihad Makhoul, Lena Torossian, Dima Dandachi and Yara Qutteina American University of Beirut, Lebanon
Study Aims To explore Iraqi refugees'
Experiences and living conditions Coping mechanisms
Background 60,000 Iraqis uprooted every month, 95% still in the Middle East Quantitative data Voices not heard No Iraqi camps UNHCR 2007
What should we know about Lebanon to help us understand the study?
Lebanon Post civil war economic decline, Beirut divided along religious sectarian lines [eastern, southern] 1951 Geneva Convention on protection of refugees declaration [not signed] Not ready to host more refugees Inadequate resources No domestic laws dealing with refugees & asylum seekers UNHCR: refugee status determination seeks solutions to resettlement papers
How did we collect and analyze our data?
Research Approach Qualitative Approach: Participants:
Informal interviews with welfare agencies 38 in-depth interviews, 5 focus groups, observations Conducted in colloquial Lebanese Arabic Thematic analysis: transcribed verbatim, coded, transferred onto spreadsheets, recurring themes.. Participants: Representatives of agencies serving Iraqi refugees Iraqi families and children [months-12 years in Leb] eastern and southern suburbs of Beirut
What aspects of the findings are presented today?
Findings.. Trail Life in Beirut Social Support Social Conditions
Paperwork Emotional turmoil Social Support
Trail: from Baghdad to Beirut
Legally Baghdad Plane Eastern Suburbs of Beirut Illegally Baghdad Syria Southern suburbs Beirut
Life in Beirut: Small unhealthy apartments, expensive rent, multiple moves "I swear to God, this is not the first apartment that we reside in. This is the fourth or fifth apartment, because we are many. The landlord doesn't accept large families, he puts them out" (An Iraqi mother) Exploitation, Stigmatization, Discrimination "There are no good jobs, I worked in many jobs but they didn't treat me well, they didn't give me good money, they didn't give me. I mean I worked in a job close by for 13 days and they didn't give me money… I mean that dessert shop and he didn't give me anything" (An 18 year old Iraqi woman) Suffering exacerbated by persecution "I am scared they would arrest us one day, I mean I am afraid that one day we will go on an outing, and then the police would arrest us and then make us go back to Iraq" (A father of six) pics
Inside an apartment in Choweifat
Paperwork 1. UNHCR paper: 2. Residency papers:
Not recognized by the State Important for settlement Humiliating Does not ensure human rights in Lebanon 2. Residency papers: Expensive work permit Require a Lebanese guarantor L
Way Out 3. Resettlement papers:
False hope, delays, frustration and ambiguity Slack global process Unclear immigration policies Contribute to family disintegration "I have been here for 12 years, how is it still being studied? 20 years to study a file?! This is ridiculous, I mean 70 staff in the agency need 20 years to study a file, if each one of them took a letter, the file would be completed in one week!" (A father who spend 12 years in Lebanon) "Once I went to ask about my file, and she told me to forget! I have a difficult psychological condition and she tells me to forget!" (A 42 year old father)
Psychosocial Well-being
Anxiety, depression, hopelessness, suicidal attempts "I am a diabetic; honestly this is due to all the work. I have a depression now. My son and I used to consult with the physician in ReStart. I would become very angry at them. I would become angry at the smallest thing and because I feel that I am the reason behind the file delay because I said the truth; I told them I was a military … Apparently the paperwork of a military is delayed… I never held a weapon in my life… I was calligrapher in the army" (60 year old head of household) Husband: "One day I woke up at 1:30 am and found that she had opened the curtains and had swollen all the pills." Wife: "I woke up and poisoned myself, I took out the pills and swallowed them all… This life- I don't want it!" Husband: "So if I didn't wake up and I didn't see her, if I didn't do something about it, she would have been gone."
What is striking in these findings?
Differences in Social Support and Coping between suburbs
Household Proximity Social Support Coping Agencies Outings Family Support Formal Informal Eastern Suburbs yes High Southern Suburbs no Low
Sud el Boushriyyeh, Eastern suburbs
FOREIGN POLICIES / MIGRATION POLICIES UNHCR Paperwork IRAQI REFUGEES PSYCHOSOCIAL WELL-BEING INFORMAL SOCIAL SUPPORT PROXIMITY SUBURB CHARACTERISTICS LEBANESE POLITICAL SITUATION Laws Sectarianism SOCIAL ACTIVITIES NGOs RELIEF ITEMS SOCIAL SUPPORT / WELL-BEING FRAMEWORK STAFF INTERACTION
What needs to be done to improve the well-being of the Iraqi refugees while in transit countries?
Implications… NGOs: State:
Use participatory research to evaluate services Train staff on interpersonal communication skills Offer rent assistance Facilitate creation of meeting spaces Increase social events/gatherings State: Integrate State services with NGO services Differentiate between war affected Iraqi refugees and illegal immigrants End detention and persecution of Iraqi refugees Allow temporary work permits
"Where is the respectful aid
"Where is the respectful aid? The coupon has nothing to do with it, I don't want the coupon. I want a house, give me a house to live in, give me residency and I would work and earn a living for my children, I would live my happiness, I would live free!" (45 year old father of 4)
Thanks to.. Iraqi refugee families and youth Non-governmental agencies
IDRC, Ford Foundation Members of the Arab Families Working Group Ms Cristel Baasiri, Ms Fadia Shoucair, Ms Samia Kallas Work in progress-comments
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- Page 1 [Introduction]
- Page 2 [The Package]
- Page 3 [Video Card]
- Page 4 [Test System Setup and 3DMark Vantage]
- Page 5 [Benchmarks - PT Boats: Knights of the Sea]
- Page 6 [Benchmarks - CINEBENCH R10]
- Page 7 [Benchmarks - World in Conflict]
- Page 8 [Benchmarks - Crysis Warhead]
- Page 9 [Benchmarks - Far Cry 2]
- Page 10 [Benchmarks - S.T.A.L.K.E.R. - Clear Sky]
- Page 11 [Benchmarks - Left 4 Dead]
- Page 12 [Benchmarks - High Quality AA and AF]
- Page 13 [Temperature and Sound Tests]
- Page 14 [Power Consumption Tests]
- Page 15 [Final Thoughts]
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Who'd guess there was a tool to convert C++ code into UML diagrams? It's called Umbrello.
Here's a simplified UML Class diagram for Vidproject and associated classes:
The Video Project has a list of the currently used Resources (video and audio clips - i.e. files ). Additionally, it contains an AVTimeline which contains various sequences. Each sequence contains various audio and video tracks. And each track contains... surprise, a clip. The clip has an index indicating which resource it operates on. And of course, a list of effects and a transition.
Notice that a video clipboard is nothing but a sequence. Convenient, isn't it?
As you can see from this diagram, the memory consumption for these classes is minimal, since they're nothing but sparse data structures.
Now, I don't really know how Cinelerra or other video editors work. But one thing's certain: It's much easier to model the classes after the structure of the data we're going to modify. And this data is a timeline.
I already have implemented saving undo/redos for these classes, and you can impose a memory limit on how many undos/redos you can store in memory. So there goes the high memory requirement. Poof! :)
I'll upload this diagram to the webpage when I can.
OpenShot 2.3 Released | New Transform & Razor Tools! - I am proud to announce the newest release of OpenShot Video Editor, version 2.3! This is one of the biggest updates ever to OpenShot, and is filled with n...
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We've worked hard to cultivate a respected reputation at the various Commissions for being well versed in the law, proper procedures, and in providing an agressive and accurate analysis of the claim.
Our workers' compensation attornies have defended a variety of claims which present issues of desputed average weekly wage, factual accident disputes, subrogation issues, temporary total disability and temporary partial disability disputes, wage loss, and permanent total claims.
Our attornies have successfully defended complex cases before the arbitrators of the various Commissions. For example, we successfully argued a disputed surgical herniated disk case in which the petitioner claimed tow separate work accidents with the same employer. Our attorney was able to successfully argue a lack of notice and insuffient evidence to prove an accident occurred for both alleged events. The arbittator ageed and ultimately found that the petitioner did not provide suficient evidence to prove an accident occurred for both alleged events. The arbirrator agreed and ultimately found that the petitioner did not provide sufient evenident to prove that an accident hadoccurred on either date adnd a zero award was receved
We've also had success in obtaining dismissals of claim on the merits of the case, as well as for a lack of prosecutio. The dismissals have often involved below the line cases, which would have otherwise been automatically continued by the arbitrators.
In accepted claims caes, we've obtained reasonable settlement amounts, well below the expected trial exposure. We've accomplised such results through preparations and by pushing the case to trial early in the typical workers' compensation case progression.
At Mulherin, Rehfeldt & Varchetto, Ltd. we pride ourselves in bringing a personal touch to each of our client relationships. We understand that by working with youon a common plan of action, we can make your job easier while providing the best, most timely, resolution.


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How Car Dealer Convince You For New Car Purchase
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More Than Conquerors
This is the only way to give it a death-blow.
Our Minds Are Like Baking Stone
Throughout our days, we are to retain the flavor of who God is, what he has done, and what he has promised.
They looked the stone over and marked it unusable, not valuable, replaceable and inadequate.
Introducing: The For The Church Podcast
Hosted by Jared Wilson, this new podcast from Midwestern Baptist Theological Seminary features reflections and conversations aimed at encouraging leaders in gospel ministry.
Present Darkness
The church should not be afraid to acknowlege the depth and range of human suffering even as we place our hope in God.
The Original Jesus Juke
Our righteouness goes beyond legalism when we obey the true intent of the law.
Preachers with Presence
Only an "in-person" preacher can bear the burdens of the congregation, weep with those who weep, and rejoice with those who rejoice.
God Does Not Answer "Selfie" Prayers
God does not answer prayers that are motivated by selfish ambition, misplaced priorities, or worldly passions.
What Christianity Offers
Life defines the Christian experience.
Episode 001: Matt Chandler
For The Church Podcast
A Resource for the Church from Midwestern Seminary
Episode 1: Matt Chandler
"If you can teach the Bible in a compelling way to 1st through 5th graders, you've got all the raw tools you need."
Episode 003: Preventing Ministry Burnout
For the Church Podcast
A Resource for the Church from Midwestern Seminary
Episode 3: Preventing Ministry Burnout
"You might say your season of life does not allow for much rest, but you are setting yourself up for a disastrous next season."
Episode 002: Matt Boswell
For the Church Podcast
A Resource for the Church from Midwestern Seminary
Episode 2 with Matt Boswell
Matt is the pastor of ministries and worship and an elder at Providence Church. A native Texan, he grew up the son of a pastor. He has been leading worship and involved in church planting since 1995. His passion is to see our congregation engaged in gospel-centered ministry, and in this environment live out the great commission. He writes songs for congregational worship…
The Former is Not The Forever
Why, O Father, must I endure this cycle of repentance in hard-heartedness forever?
The Sluggard's Substitute
In His life, on the cross, and in His resurrection, Christ labored to accomplish salvation for spiritual sluggards like us.
Checking Our Compassion Gauges
Let us be radically compassionate because of the radical compassion we have richly received from Jesus.
Redeemed to Perpetuate the Name
Do you know the name of the kinsman redeemer who was first in line to marry Ruth? Exactly.
The Mission Field Under My Roof
The home is where stories are taught, faith is worked out, neighbors are heard, plates are filled, and where sojourners can find rest.
Links For The Church (4/17)
This week's links include resources on pain, union with Christ, dying, students and missions, and historical evidence of Jesus.
Rise, for the Lord is Risen!
The resurrection is the corner-stone of the entire building of Christianity.
What Do You Do When You're In the Pit?
Learn to groan in the pit, but never grumble!
The Most Important People In The Body Of Christ Are Probably Not On Twitter
Simple math tells us that it is faithful, ordinary, anonymous lay people who make up the vast majority of the churches around the world.
Pastor, Defend Christian Liberty
Defend your people's freedom in Christ with your life if necessary. But do not impinge on their freedom, even if you think it's for their own good.
Encouraging a Plurality of Elders
Plurality is possible. God commands it. Let's pursue it and protect it.
The Freedom of Love
True love makes obedience a joy-filled pleasure.
The Exclusion of Christ
The Lord knocks at their door in order to remind them of who they forgot.
Provided We Suffer With Him
Suffering does and should cause us to ask deeper questions about life and the nature of God.
Man-Made Coverings
God's goal for us as his children is not to conform us to Christian culture, but to his Son.
The Messy Blessing of Multi-Gen Groups
Blending age-groups in our ministries helps us accurately represent the diverse spiritual family that God has given us.
Why Do We Read the Bible?
As much as reading the Bible is an intellectual exercise, gaining knowledge shouldn't be the main reason we read it.
Michael Kelley on What Many Churches Get Wrong About Discipleship
We ask Michael Kelley, "What do too many churches get wrong about discipleship?"
Raising Expectations for Young Believers
These young believers possessed a demonstrable commitment to personal and corporate growth in their walks with Christ.
Advice on Hosting and/or Accepting Speaking Engagements
If you're interested in hosting a conference or otherwise hosting a speaking engagement, or if your ministry platform is allowing you to begin pursuing God's call into that option, I hope this advice will serve you well.
Links For The Church (4/10)
This week's links include resources on believer's baptism, perfectionism, loving your neighbor, and gray hair.
Greatness by Goodness
How marvellous has been our experience of God's gentleness!
Why I Admire Pastors and You Should Too
Most of the pastors I know garner my trust and respect and deserve my prayers and support.
How Can A Pastor Effectively Counsel Someone Without Creating A Co-Dependency?
Series: Conversations
5 Discipleship Don'ts
Discipleship is about helping a disciple faithfully relate to Jesus, not to you.
Brother, I Wish I Would Have Known
Be that friend. Love the addict. Remind them of the gospel - that there's another way that is better.
Remember the Sabbath: Part 1
Part 1 of a 4-part series on leadership and rest.
God Loves Us Enough to Disappoint Us
The very worst thing God could do for us is give us the life we want in our flesh.
Sex: The Cathedral of Our Culture
Sex simply cannot sustain the weight of our culture's expectations.
Do You Invite Critique of Your Sermons?
As pastors, should care more about getting the Bible right than simply feeling like we're right.
The Unlikely Saint
One enters into eternal peace and joy. The other? "Depart from me. I never knew you."
Soli Deo Gloria
For all His love for humanity, God did not ultimately preserve a nation for the sake of sheer benevolence. He did so for His glory.
Principles for Transitioning to Multiple Elders
Moving to a plurality of pastors is a good goal that is worth pursuing, but it's important to not get in a rush.
Dan Darling on the Church's Support of Parachurch Ministries
We asked Dan Darling, "How should a church decide which, if any, parachurch ministries to support?"
The Gospel's Compelling Uniqueness
The safe Jesus of modern evangelicalism is not offensive, but neither is He very compelling.
There Once Was A Farmer
His pleasure was rooted in the harvest he hoped for.
Links For The Church (4/3)
This week's links include resources on redefining ministry, protecting your children, folding singles into the family, anxiety, and leadership credibility.
Pray for God's Glory, Not Your Own
Such a petition, fervently presented, will elevate the spirit of all your devotions.
What is The One Thing the Local Church Needs to Get Right in the 21st Century?
Michael Kelley Lays Down Some Truth
Series: Conversations
You must get this right.
What Is The Bible And What Does It Tell Us?
If we want to learn more about who we are, we don't look within, instead, we look outside ourselves to God's Word
Sharing the Gospel at Work
You are not at your workplace by accident but by divine providence.
3 Reasons Young People Leave the Church
70 percent of 18–22 year-olds stop attending church for at least one year, and a majority of 20 year-olds leave church, often never to return.
The Pronouns of the Gospel
If we're the people who are reconciled to God, that reconciliation must reveal itself through our relationships within the church.
4 Reasons Your Work Matters Today
All of us, when the work slips into tedium and routine, are prone to ask the question of whether or not what we are doing really matters.
Why Artists Need Theology
The truth of the matter is that there is no ground for creativity that comes close to the intellectual fertility of Christian theology.
No Spin on Sin
The Holy Spirit progressively conforms Christians into the image of Christ.
The Right Side of the Line
The line between noble intentions and prideful purposes is so difficult to see that it's easy for us to cross.
How to Grow Your Church Numerically
Whenever true disciples are added to the church, the gospel has been proclaimed.
The Gospel in 140 Characters
The gospel is bigger than the universe, but can it be tweeted in 140 characters or less?
H.B. Charles on the Pastor's Most Important Weekly Tasks
We ask H.B. Charles, "What are the pastor's most important weekly tasks?"
Jesus Is Better Than Our Dreams
The Church should cradle the richest, most outlandish dreams.
When You Feel Forgotten, Abandoned, and Unloved
This does not make pain painless, of course, but it doesn't make it purposeless either.
Links For The Church (3/27)
This week's links include resources on lamenting, singing at a new church, flirting, and sour desires.
Freedom is Coming
The Surety is bound, and justice demands that those for whom he stands a substitute should go their way. In the midst of Egypt's bondage, that voice rings as a word of power, "Let these go their way."
Production or Virtue?
If I prioritize production, I end up frustrated, and I actually abandon virtue and toy with anger or bitterness.
Keep Going Out and Keep Sowing
It's not the tears that produce fruit, or even the faithfulness of the sower.
Scripture in Songwriting
Writing music for your people is a challenge - it is a heavy responsibility to put words and thoughts about God into people's hearts and minds.
To PhD or Not to PhD
A few reflections on whether or not pursuing a PhD is a right fit.
One Problem With Being an Insecure Minister
The more we trust in the sand of our own efforts, the less secure we'll actually be.
When God Gives You "X" But You Asked For "Y"
God exposed my misplaced allegiance by closing doors that, if opened, would've led down a path of continual self-destruction and false worship.
Making A Pastoral Apology
If you're a leader, others might have been involved in the mistake, but they were likely following your lead.
Counsel For The Counselor
In prayer we are able to find comfort in the arms of our Father, trusting that he is working all things for the good of those who he loves, especially those he has entrusted to our pastoral care.
On Emotionalism and the Gospel
Leaders, trade in the tricks of the trade for the all-powerful, all-surpassing glory of Jesus Christ in the gospel.
Oddly Fashioned Heroes
More than likely, you and I are the products of the quiet faithfulness of a generation of men and women whose names the world will never know.
Adam McClendon on How Churches Can Appeal to Both Young and Old
We asked Adam McClendon, "How can a church stay attractive to younger people without alienating older people?"
Links For The Church (3/20)
This week's links include resources on conviction, congregational singing, preaching race, and weary moms.
Thoughts on Note-Taking During Sermons
If you are only taking notes during the sermon because you feel you must, because you treat the sermon like a lecture or "lesson," I would encourage you to reconsider.
Tale As Old As Time
The gospel is the ultimate "tale as old as time" because we get God.
Faith Engages the Omnipotence of God
If we want blessings from God, nothing can fetch them down but faith.
Learning From Lifetimes of Experience
God's Word is able to comfort and counsel without lecturing because it flows from people's real life experience, not their bare philosophical reflections.
What Preachers Can Learn From George Whitefield's First Sermon
Abundant religious activity cannot mask a rebellious heart at the last day.
2 Good Reasons to Say "Amen" Today
There are only two options with Scripture: either we say amen to its entirety, or we entirely reject it.
How Have I Loved You?
Let us embrace the love we don't deserve and love our Father because He first loved us.
Living to Honor, Not to Earn
My friends, let us be a church that savors, promotes, and clings to grace.
Adoption is Commitment
I want the Father's adopting-love ingrained into the very fabric of my thinking
Feminism and the Fall
While we must fight against the oppression and violence that women experience around the world, we must be careful not to venture beyond that into a realm of self-exalting and unbiblical femininity.
Do You Desire The Ministry?
There is no such thing as a pastor who knows only the burden of leadership or only the sweat of service.
Reclaiming Your Eyes from Pornography
Let the unveiled image of Christ burn into the eyes of your heart. This is the hope of transformation.
Praying to Walk Right
This is the believer's confidence in the Lord. God alone is my help and my hope.
David Prince on the Church and High Impact Sports
We asked David Prince, "What should the Christian think about high-impact sports?"
Jesus is a Straight Shooter
For our joy, Jesus always shoots us straight.
Links For The Church (3/13)
This week's links include resources on rest, childlessness, spiritual strength, and a forgotten hero.
Making Real Friendships In The Church
We each echo God's glory differently for the sake of the Kingdom.
Nothing is Neutral
No neutralities can exist in religion.
Into the Great Unknown
Go to the source. Go into the dark of things you don't know. Watch how the stars shine.
Is Your Platform Worth Your Life?
Christians, how can we be vigilant to guard against selfish ambition in our lives and ministries?
Jonathan Leeman on Elders and Deacons Working Together
How can a church avoid turning its elders and deacons into a sort of bicameral legislature?
Series: Conversations
Helping Our Daughters To Be Sexually Pure
The way to help my daughter pursue purity isn't to lock her up in a castle, but to help her walk in the freedom of the gospel.
On Malachi and Religious Ritualism
Let us learn from God's words through Malachi and seek out a true relationship with God characterized by Jesus' command to love God and love our neighbor.
Confrontational Love
A community that lives with love enough to both provide and receive gentle rebukes from one another makes for an incredible family.
5 Reasons You Desperately Need Your Bible
Until our race is finished, we glean from him, day in and day out.
Suffering is a Doorway, Not a Dead End
Because Christ walked ahead of us, we know greater fellowship with Him as we follow in his steps.
The Gospel Is Bad News For Our Stereotypes
The work of Christ applied to our hearts is such an unstoppable, unopposable force that it refigures us entirely.
11 Characteristics of the Self-Righteous
In an effort to help us create self-awareness and keep us out of the trap, here are 11 characteristics of the self-righteous . . .
Gospel Culture
The culture that we allow our doctrine to create is the real test of what we believe the doctrine to be.
J.T. English on Growing Churches "Feeling Small"
We ask J.T. English, "How do you make sure a growing church keeps 'feeling small?'"
Links For The Church (3/6)
This week's links include resources on integrity, home, small group woes, and knowing God.
Men Wanted: Come and Die
Men wanted for hazardous journey. Small wages, bitter cold, long months of complete darkness, constant danger, safe return doubtful. Honour and recognition in case of success.
A Small Church in a Mega-Church World
God delights to work through weak vessels that are small in the world's eyes.
Lord, Give Us the Sense That We are Yours
Let me have a present, personal, infallible, indisputable sense that I am thine, and that thou art mine.
The Necessity of Prayer in Sermon Prep
We will be as prayerful as we are cognizant of our own weakness.
Beauty for Ashes: The Groom and His Bride
His perfection becomes hers when he takes her filth with him in death.
In or Out?
You are in Christ and fully in, or you are out and fully out.
Kids, Asparagus, and the Command of Jesus
The commands of Christ feel crushing without a new heart to help us obey.
Come Behold the Wondrous Mystery
A few reflections on a modern-day hymn.
He Is Your Praise
God gave Jesus because Jesus is the highest form of praise I could ever receive.
Enjoying Marriage Through the Lens of the Gospel
To fully enjoy one another is to view them with new eyes, saturated in the grace of our Savior.
How to Leave a Church
If you are a leader and you've led them thus far, lead them further by giving them some space to plan, pray, and get collected.
4 Reasons to Preach Christ from the Old Testament
But there is much more to the Abraham narratives than a simple "do this; don't do this."
Praying Specifically
What if we walked into every day with the expectation that God hears and answers specific prayer?
Michael Kelley on the Writing Process
We ask Michael Kelley to describe his writing process.
Dear 'Concerned Church Member'
It is Jesus who is available to you 24/7, and I'm not him.
Links For The Church (2/28)
This week's links include resources on social media, raising faithful kids, lessons for American Christians, and the benefits of discipleship.
Beware of Ministry Shaming
If we strive to build up one another in our ministries and tune into other people's feelings, we can help the body of Christ feel unashamed for the call given to them by God.
God Saves You All by Himself
Whatever I have, all my goodness is of the Lord alone.
Are You Suffocating Your Creativity?
If we allow ourselves to be diverted and distracted all of the time we will scarcely enjoy the deeply meaningful seasons of discovery that come from thoughtful reflection.
6 Tips for Preaching in a Missionary Context
We should strive to preach our best sermons in overseas contexts.
Russell Moore on the Culture War
Series: Conversations
If conservatives have lost the culture war and the American church is in decline, what is there for evangelicals to be encouraged by?
Join the New England Study Tour This May!
Deadline to Register is March 1
Walk where Edwards and Whitefield walked, visit everywhere from Yale to Harvard and coastal Maine to rural Vermont, meet with local pastors and church planters, and even enjoy some local eateries and coffee.
At Your Wits' End
Mark this down: the Lord hears and answers the cries of desperate people.
When We Open the Bible
To read the Bible is a risky thing. We cannot read it, put it down, and claim ignorance.
Rules Without Reasons
Sometimes, we won't think there are good enough reasons given to obey God, yet we are called to obey Him anyway.
Angry Twitter and Confused Christians
While important matters call for our earthly attention, we cannot forget that the church's unique commission is to promote a kingdom that cannot be shaken.
When You Are the Old-Timer
We ought to be the most hospitable toward the sojourners among us.
Love for the Forgotten Pastor
The promise of the gospel is not that man will remember you, but that God knows you.
What Will Your Children Remember About You?
When your children grow up, and they are asked, "Tell me about your dad" or "Tell me about your mom" - what do you think they will say?
Why Should One Consider Formal Theological Education?
We ask Nathan Finn, "Why should one consider formal theological education?"
Links For The Church (2/20)
This week's links include resources on entertainment, letting God, the Trinity debate, and singleness.
Letter from a 'Concerned Church Member'
We really do want the best for you, which is why we felt like you needed to know these things. With a little more hard work and concentrated effort on your part, this can be a win-win for all of us.
Thankful for His Keeping
The gift hidden within difficult seasons is that we may see more clearly and understand more deeply our desperate need of His keeping.
Grace is Meant to be Shared
I will not believe that thou hast tasted of the honey of the gospel if thou canst eat it all thyself.
For Better Conversations
Your artful questions and interest make both sides of the conversation possible.
Health, Wealth, And Not Much Else
Revival wasn't shouting and screaming; it was the beat of love within me for others.
One Thing: Devin Maddox
Series: One Thing
How can a church engage in social justice without falling into the errors of the social gospel?
How to Get Your Prayer to Work
The power to answer prayer belongs to God alone. And the glory for answered prayer belongs to God alone.
Expositional Imposters
Seven pitfalls to avoid when preaching expositional sermons.
Jesus Walks Amongst the Churches
Jesus is not far-off, peering through a telescope. He is among the churches. And He is with us now.
The Insensitivity of the Cross
God was mercifully insensitive to our feelings and desires at the cross.
For Young Adults Considering Ministry
Is your greatest desire to see the gospel go forward in the place where God has you?
God Still Seeks Wandering Fools
The incarnation glorifies the extent to which God would go to seek out those who were running from him.
Christians and the Realm of Desperation
If we are to follow Jesus, then we must have a growing understanding that the Christian does not operate in the realm of desperation.
Be a Humble, Lifelong Learner
There is something freeing when you humble yourself and place yourself before a group of people who say, "We see this area that needs to be worked on."
Clint Pressley on Leading Big Change in the Local Church
We ask Clint Pressley, "How do you lead big change in a church without blowing it up?"
Pastor, Keep A Close Watch On Your Life and Illustrations
You shouldn't trust your illustration to do what only God's word can.
Links For The Church (2/13)
This week's links include resources on spiritual formation, joy, women's roles, and multi-ethnic ministry.
Do You Pray For Your Competitors?
What if we refused to be threatened by our sister churches and instead committed to pray for them?
He Shall Give You a Comforter
The Holy Ghost is no temporary gift, he abides with the saints.
Pastoring People Through Slow Change
The call to plant and pastor is primarily a call to help people keep the gospel in focus.
Pastors and the Problem of Evil
Our people need the hope of the cross and the assurance of the resurrection.
One Thing: Lane Harrison
Series: Conversations
What are the pastor's most important weekly tasks?
Diary of an Ex-Shepherd
"First day on the job with the new flock. It isn't the biggest or the healthiest flock in the world…but it's mine. And I'm excited to see how they'll grow."
How Can I Delight in God's Word?
When the Bible is our delight, we look forward to reading it each day and feel a keen sense that something's missing when we don't.
The Gospel and Social Media Part 4
Thinking through these questions often can help identify your stream as a gospel-filled well your followers should drink from often or a gospel-less pit followers should avoid.
Song Requests and Gospel Rest
Ultimately, God's glory in your selections should be more important than making someone happy
When You Are The New Kid
Roll up your sleeves, labor alongside them, and love them.
Dear Bi-Vocational Pastor
There is no nobler task than to deliver good news to weary souls week-in and week-out.
What's Left When a Church Closes
The danger of focusing on things over people is that we might actually get what we want.
Photobombing Jesus
Confessions of a Glory Thief
A simple, but profound truth: Jesus came to save glory thieves from themselves.
J.D. Greear on Sharing the Gospel with Muslims
We asked J.D. Greear, "What are some important things to remember when sharing the gospel with Muslims?"
10 Books for Your Church's Book Table
I have composed this list thinking of the kinds of needs and interests most Christians seek out answers for in the sorts of books that sell most often at Christian bookstores.
How Journaling Combats Sin
Journaling is not an end in and of itself; the end is to look to and live by the cross.
Links For The Church (2/6)
This week's links include resources on self-care, abortion, our problem, and community.
The Father Sent the Son to be the Savior of the World
Didst thou ever consider the depth of love in the heart of Jehovah, when God the Father equipped his Son for the great enterprise of mercy?
When They Say Parenting Gets Worse
You don't get to decide to lay down your life one day and then not think about it again
How important is it for preachers to pay attention to cultural trends and popular media?
Series: Conversations
How important is it for preachers to pay attention to cultural trends and popular media?
The Gospel and Social Media Part 3
In a thread of confirmation bias, hate, uncareful thinking, narcissism, and over exaggerations, be the voice of gospel reason.
Stott's Simple—Yet Surprising—Productivity Secret
Hitting the pause button on life and ministry will help us find rest for our souls and experience a sweet foretaste of heavenly rest in Christ's presence.
Community Requires Vulnerability
Community isn't something that comes to us; it's something that we go toward.
Gospel-Division That Creates Gospel-Ambassadors
Belief in the gospel is the only way anyone can cross the gospel line and experience eternal life.
God Will Give You More Than You Can Handle
We've heard it said, "God will not give you more than you can handle." Truthfully, God can and does give us more than we can handle - all because it leads us back to Him.
What are some common rookie preaching mistakes?
We asked H.B. Charles: "What are some common rookie preaching mistakes?"
Christian Life Beyond the Quiet Time
Devotion as a Way of Life
Be filled with the Spirit? How can I be something the Spirit does?
Links For The Church (1/30)
This week's links include resources on womanliness, accountability, seminary, and presence.
Looking to the Future with Hope
The joys of heaven will surely compensate for the sorrows of earth.
Redeeming 'Whatever' (Part 2)
When we ponder the right things, we proceed in the right direction.
What Are The Pastor's Most Important Weekly Tasks?
We asked Noah Oldham, "What are the pastor's most important weekly tasks?"
Series: Conversations
What are the pastor's most important weekly tasks?
The Gospel and Social Media Part 2
Remember, a cross and the pursuit of the good of others should mark your Christian life. This must be true for your social media presence as well.
Leading Meetings with Humility
As we acknowledge the need to lead humbly, what does godly, humble leadership look like in the day-to-day?
How to Practice Christian Encouragement
I believe encouragement should take its place alongside any list of spiritual disciplines.
The America We Forgot
There is much wisdom when you have a love for people wherever you find them, urban or rural.
5 Words That Should Scare Your Pastor
Five little words that once fed my pride in ministry are now terrifying to me.
How can a pastor effectively counsel someone without creating a co-dependency?
We asked Adam McClendon, "How can a pastor effectively counsel someone without creating a co-dependency?"
What if You Aborted a Future Janitor?
Why We Should Reject Utilitarian Arguments Against Abortion
Christians should have nothing to do with utilitarian arguments against abortion.
Links For The Church (1/23)
This week's links include resources on the rich, waiting, masculine friendship, and heresy.
Why You're Suffering
Sooner or later every bar of gold must pass through the fire.
3 Principles to Communicate the Bible
All of us... are communicators of the gospel.
How can smaller churches conduct successful ministry training?
How can smaller churches conduct successful ministry training?
Exulting in the Shepherd
Psalm 23 has a lot to say about Christ's tender, loving care for us. But the psalm isn't primarily about the sheep; it is about the exaltation of the Shepherd.
The Gospel and Social Media Part 1
Your justification isn't in the fact that you don't post selfies or engage in over-the-top political comment streams. It's in the blood of a murdered Son.
Church, This is a Call for Familial Friendship
We must actively pursue deep relationships with others within our churches in which we bravely share how the gospel lays claim to our lives.
A Question of (Alt) Right and Wrong
Christians must hold to the higher standard that all believers are one, a new race in Christ.
The Danger of Entertaining Lies
It would be convenient if Satan would show up as a talking snake. Then, we could run away screaming or call animal control. Instead, he comes to us as an agent of reason.
Who is a criminally under-appreciated figure in church history we should know more about?
We ask David Prince, "Who is a criminally under-appreciated figure in church history we should know more about?"
Are You a Confirmation Bias Christian?
How would you know if your Christian life has become hijacked by confirmation bias? Here are some possible diagnostic signs . . .
Links For The Church (1/16)
This week's links include resources on feeling dead on the inside, pro-life causes, repetition in worship, and teaching children about hell.
Give Yourself to Prayer
The common fault with the most of us is our readiness to yield to distractions.
One Thing: Jonathan Leeman
How do you lead big change in a church without blowing it up?
Series: One Thing
How do you lead big change in a church without blowing it up?
Redeeming 'Whatever' (Part 1)
How does God reach us when our heart staggers mindlessly into the gray dusk of 'whatever'?
8 Important Things About the Young Timothy
Feel you aren't useful? Need a reminder of God's methodology? God uses the willing. God uses the available. God uses the weak.
The Essential Marks of a Preacher
If God has called you to be His preacher, never stoop to be a king of men.
Christians in La La Land
The real call of Christianity is to find our love and meaning by trusting in, submitting to, and abiding in Christ.
8 Books That Have Influenced Me
I would argue that a book is only truly influential in so far as it somehow moves into your life without you asking it to.
Don't Pollute the Stream
If I had a few minutes to sit a new minister down and share a few things I've learned, there is one piece of advice that I believe is vital.
5 Things The Seeker Movement Got Right
In the spirit of charity, seeking to identify some good gifts the seeker church of yesteryear has given contemporary evangelicalism
Links For the Church (1/9)
This week's links include resources on curiosity, congregational singing, procrastination, and weakness.
Are You Unholy About Holiness?
Even our desires after holiness may be polluted by ill motives.
Are You Allergic to God's Judgment?
Declawing the Bible of its warnings against and judgments upon sin is not good for the church.
I'm Taking Back Everything The Devil Stole From Me!
Let the church say, "Huh?"
Community Keeps You From Drifting
All Christians want to stay close to Jesus. But to try to do that on your own is to set out to fail.
Friendship for the Pastor's Wife
The pastor's wife is just like every other woman in the church when it comes to friendship: she gets a choice who she lets in.
Caution and Help for the Hyperdriven Leader
There are leaders that propel their churches forward at breakneck rates helping them journey great distances and achieve impressive results. Though they often refer to themselves as "visionary leaders," I would argue that many of them are more hyperdriven than visionary.
A Better Temple
As beautiful as the temple was, it was but a shadow of the magnificence of Christ.
Ministry Isn't an Individual Sport
Is your ministry an individual sport or a team sport?
The Resolutions of Jonathan Edwards
I wonder how the resolutions of us donut-eaters might stack up to these from Jonathan Edwards.
Links For the Church (1/2)
This week's links provide resources on interpreting the Bible, true masculinity, resolutions, and Christology.
Let January Open With Joy
A New Year's Devotion from Charles Spurgeon
We open the gates of the year to the sweet strains of the harp of joy!


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Your Overtons.com Session is About to Expire!
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Share your pictures
of life on the water.


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recommended by Barb M., Keyboard and Folk Music Specialist The Hal Leonard Guitar Method is designed for anyone just… read moreApril 19th
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Series: Playground String Orchest Price: $42.00


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When life squeezes you, self-discovery is likely not on your short list of coping mechanisms. Yet your most valuable response is to explore the inside of this equation: you. How do… Continue reading
Can you be of service to anyone except yourself?
How can you be in service? What does "being in service" mean to you? Consider these Does it involve sacrifice on your part? Does it include offering advice,… Continue reading


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Can Micro Chinese Medicine be Taken Along with Western Medicine2016-05-02 16:13
Can Micro Chinese medicine be taken along with Western Medicine? In general, Micro Chinese Medicine can work well with the Western Medicine, what's more, this treatment can relieve those side effects caused by western medicines, thus to get a better curative effect.
What are the Functions Of Micro-Chinese Medicine Osmotherapy?
1.Prevent renal function from deteriorating
Micro-Chinese medicines are featured with dilating blood vessels, anti-inflammation, anti-coagulation and degrading extracellular matrix. After the effective medicines are penetrated into kidney lesions, they can stop the releasing of inflammatory factors and clear up harmful substances to kidneys. In this way, it can halt the renal function to deteriorating immediately and effectively.
2.Active the self-replication of renal functional cells
Micro-Chinese Medicine Osmotherapy can activate the self-replication of renal functional cells and boost the reconstruction of kidney structure. The active materials in Chinese medicines can block renal fibrosis, dilate arteries at all levels, improve the regeneration and differentiation of renal functional cells. Meanwhile, the medicines can promote the secretion of growth factors so as to activate the formation of new blood vessels and accelerate the reconstruction of glomeruli.
3.Improve the metabolic disorders and provide an aerobic environment for repairing the impaired renal functional cells.
The effective medicines can dilate blood vessels at all levels and increase blood and oxygen supply to kidneys. In this way, it can create an aerobic environment for restoring the impaired kidneys. As renal ischemia and anoxia is the root cause of renal fibrosis, once these conditions are reversed, the kidney function will stop deteriorating.
If you want to know more details about Micro-Chinese Medicine Osmotherapy or want to control kidney failure well, you can contact us by the following ways:
Send email to firstname.lastname@example.com
Consult our doctor directly on WhatsApp/Viber: +8618633865632
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text: The color scheme of traffic lights is the result of borrowing from railroad signaling schemes of the time, according to Mental Floss. The first traffic lights were green and red, and amber later became the third color to provide a buffer between the "stop" and "proceed" signals. This was to prevent accidents due to sudden light changes as well as to allow the policemen time to switch the lights manually.


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But since they had no choice, they were glad to have options when Esteban Loaiza and Rich Harden were placed on the disabled list within a three-day stretch.
"We built this team for this exact scenario," A's assistant general manager David Forst said.
"We've got at least four guys in the bullpen who have started major league games and are fully capable. Hopefully they will seize this opportunity and keep us afloat."
So far, the results have been positive.
Brad Halsey, taking Harden's place, allowed three runs on five hits in 5 1/3 innings Tuesday in the A's 10-3 win over the Angels, leaving with a 4-3 lead. He took the loss on Sunday, but allowed only one earned run in the A's 3-2 loss.
Pitching in Loaiza's spot, Kirk Saarloos allowed one run on eights hits in five innings. It capped a seven-day stretch in which Saarloos saved a two-run game, finished a seven-run game, and started a game.
"That is cool," Saarloos said. "Save a game. End a game. Start a game. Win a game. What's next? Maybe I'll get an at-bat."
Saarloos wasn't bummed when the A's chose Halsey to pitch the first game. He was told there would probably be two openings in the rotation anyway.
"Brad's thrown the ball great and he deserves (to start) as much as anybody," he said. "We're here to win. It's not about my agenda."
Statements like that endear him to manager Ken Macha.
"Baseball's not always a fair game," Macha said.
"His attitude is exemplary. He'll do whatever it takes to help the team."
Pitching Depth Shows Up Early
Oakland Clubhouse Top Stories
Oakland A's MLN: Bassitt begins comebackChris Bassitt's first rehab start highlights a news-filled day in the Oakland A's system.
Oakland ClubhouseYesterday at 8:44 PM
Oakland A's MLN: Sanchez to DL; Olson to NashThe Oakland A's have made several roster moves up and down their organization recently.
Oakland ClubhouseThursday at 1:35 PM
Shoulder a concern for A's ManaeaOakland A's left-hander Sean Manaea left his Wednesday start early with shoulder discomfort.
Oakland ClubhouseThursday at 6:03 AM
A's DL Davis; Alcantara DFAdThe Oakland A's made several roster moves on Tuesday in advance of their series opener with the Los Angeles Angels.
Oakland ClubhouseTuesday at 4:00 PM


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Algonquian Tribes | Communities | First Nations | Ojibway Indians History | Reservations | Tribes
Rocky Boy Tribe of Chippewa Indians of Montana Needs Your Help
Rocky Boy Tribe of Chippewa Indians of Montana needs funding to establish offices at Blackfeet Reservation, Crow-Northern Cheyenne Reservation, Flathead Reservation, Fort Belknap Reservation and at Great Falls, Montana where Hill 57 Reservation is located. Our goal is to gain Tribal Recognition at Blackfeet Reservation, Crow-Northern Cheyenne Reservation, Flathead Reservation and Fort Belknap Reservation and Federal Recognition for Rocky Boys Tribe of Chippewa Indians at Great Falls with Reservation. Your donation will be greatly appreciated. Below is my paypal link where you can donate to this very important cause for survival. If you are interested in becoming a member of Rocky Boys Tribe of Chippewa Indians of Montana, you can fill out a form here . In comments box, please include your tribal affiliation. In Montana, members of Blackfeet, Crow-Northern Cheyenne, Flathead, Fort Belknap and Rocky Boys Reservation are automatically members of Rocky Boys Tribe of Chippewa Indians of Montana. However, if you are a member from another tribe (Reservation) your application will be approved if you have proof of membership from your tribe (Reservation).
Click Here To Donate
Ojibway Indians of Florida
How, you may be asking, did the ojibway indians of florida get there? After the Ojibway defeated the Iroquois League in the first part of the devastating Beaver Wars (1667), they were driven by prophesy to invade towards the south, into what are now the States of Alabama, Georgia, Kentucky, North Carolina, South Carolina, Tennessee and Virginia. The whites refer to these southern Ojibways as Shawnee. We know the Shawnee are Chippewa. They speak Chippewa according to the 19th century book "History of the Ojebway Indians" by Ojibwa author Peter Jones. We also know the Yuchi Tribe are really Ojibway. According to Bartram, the Yuchi either spoke a dialect of Shawnee or spoke Shawnee. So they (the Shawnee) are not a distinct tribe but were Ojibways who were in the south to defend theirs, and their fellow Indian tribes homelands, against the invading whites. A little after the War of 1812 ended, the whites pursued the fleeing Ojibways, their black allies and Indian allies, down into Florida. After the first Seminole War ended the whites granted the Seminole a large Florida Reservation but a few years later stabbed them in the back, which led to the second Seminole War. Around 1819 the first Ojibways, their black allies and Indian allies, had constructed large boats to flee to the Bahamas (Andros Island) to escape from the whites. Below is a list of the Chippewa's who presently live in Florida. They are the Miccosukee and Seminole Peoples. Black Seminoles have a 50 acre Reservation at Fort Pierce Reservation.
Big Cypress Reservation
Size: 82 sq. mi.
Population: It is 493 according to the 2010 census
Language: Ojibway
Brighton Reservation
Size: 36,000 acres or 36 sq. mi.
Population: It is 574 according to the 2010 census
Language: Ojibway
Fort Pierce Reservation (it is a black Seminole Reservation)
Size: 50 acres
Population: It is 45 according to the 2010 census
Language: Ojibway
Hollywood Reservation
Size: 497 acres
Population: It is 496 according to the 2010 census
Language: Ojibway
Immokalee Reservation
Size: ?
Population: It is 103 according to the 2010 census
Language: Ojibway
Miccosukee Reservation
Size: 128 sq. mi.
Population: It is included with Big Cypress Reservation
Language: Ojibway
Tampa Reservation
Size: ?
Language: Ojibway
Coconut Creek Trust Land
Seminole Trust Lands


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how can I find out what dealership my car was sent to I have an order number on my fender tag
1 Answer
Hello, This is a toughy. You will have to contact Dodge and see if they can help with this one. VINs can reveal where a car was produced and assembled, but not dealership location. The order number may be able to provide some assistance, but Dodge itself would be the source of this information. Best of luck! CarGrrl
0 out of 1 people think this is helpful.
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There is unspoken beauty in being the only one like me.
When negativity presents itself in my life, I draw on my inner strength to rise beyond the issues at hand.
In any challenging situation, I take time to consider all the things that make me special and to remember my own worth.
I continually acknowledge the aspects of me that make me proud of my existence. I actively avoid allowing others to downplay my value.
Although I know my own worth innately, I also see it reflected in my dealings with others. Each day, as I interact with others, I love myself more and more because I realize that nobody contributes to their lives in exactly the way that I do.
Today, I commit to embracing my uniqueness, regardless of anything others may say. My belief in myself and my own
worth brings love not only to me but also to those around me.
Because I cherish myself, I spread joy wherever I go.
Talking with Myself:
1. How do I feel when others are positively affected by my uniqueness?
2. Why is it important for me to focus on my positive attributes?
3. What unique traits of mine can I demonstrate today?
Tonya GJ Prince
Latest posts by Tonya GJ Prince (see all)
- 21 Affirmations that Summon Serenity - April 19, 2017
- Survivor Affirmation Video:I Am My Own Best Cheerleader - April 12, 2017
- Survivor Affirmation: I Love Myself Because (video) - April 9, 2017


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Discussion in 'SiriusXM Sports' started by semipenguin, Aug 4, 2016.
Jurgen Klinsmann fired as USMNT coach
Sergio Agüero helps Manchester City pull it out of the fire against Monaco | Football | The Guardian
Dear SiriusXM,
Not broadcasting Drivetime from Talksport of a morning is daft ..... and worst still is time-shifting the program. It's a morning staple.
This is probably one of the reasons the Motherwell gaffer was giving the flick.
No own goal headers
.... it was one way to stun the crowd
Dear SiriusXM,
Using a TV audio feed on radio for football (soccer) is just plain daft. At what point in the English language does one hear the phrase "I listened to the video highlights - they were great!" . Surely Fox or anyone for that matter is offering up an actual radio commentary, you wouldn't run NFL or college football with a tv fee, why is the round ball different?
End of rant
Twitter Villa highlights vs Norwich
Worth a watch for .... the penalty that wasn't - even the Ross County gaffer agreed - and then Scott Browns red card for taking a Liam Boyce out in revenge - he is now out of the crucial Scottish Cup semi against Rangers.
Ross Country vs Celtic
THAT, was not a foul.
Great acting, tho. He really sold it
... and the outcome.....
Ross County striker Alex Schalk handed two-match ban for diving in Celtic draw
Second City Derby
Aston Villa 1 - 0 Birmingham City


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Do you guys really want to go there?
Lois Uttley notes that GOP strategist Frank Luntz is formulating the Republicans' scare tactics to stave off health reform. Among the word games, Luntz urges Republicans to speak out against "interference from insurance companies and Washington politicians and special interests" in health care. He says Republicans need to portray themselves as defenders of the "sacred doctor–patient relationship." (Read Luntz's full memo at Politico.com.)
Interference from insurance companies: is that like when they tell us which doctors we can and can't see? And about that sacred doctor–patient relationship: will the South Dakota GOP keep that talking point around for Leslee Unruh's next big abortion ban?
It took 7 years, but the mayor finally admits to his Hubris - Before we get to the mayor flapping his trap and not really filling us in on what he is running for, I still predict he will be running for Congress. Once ...
10 hours ago


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29403, SC Rent to Own Homes
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Triple Net Land Lease - Investment Sale. Wonderful opportunity to purchase 1 of the most accessible
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opportunity to own three new multiple luxury units in Downtown Charleston. Each unit comes with 4 beds and 3
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.**** Commercial Land Sale or Ground Lease. Call For Offers Deadline is May 8, 2017 5PM EST. Unique land
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additional parking spaces available for lease in the adjacent garage.
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house for rent and 3) 1,750 SF 2 beds/two bathrooms duplex. Frontage on Meeting & Poinsett Street
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NOI: $706,311.00 Signed CA required to view financials. (CA attached) 146 Fairchild Street is a multi-tenant Class A office building...
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146 Fairchild St Charleston, SC 29492Details »
Rd Overlay. Currently leased to a landscape company for 800/mo and until March 2018. The adjacent
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), and a vacant lot in between (currently leased to landscape company). Both houses are rented for $1
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Turnkey catering building for sale or lease, available for immediate occupancy. Located on James
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. Building C consists of four units and is currently leased (lease renewed and expanded in August 2015
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An 8099 SF section of the Parkwood Center on Savannah Highway in Charleston. This section is a stand alone building as the entire...
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will continue to do lease back for his medical practice. Tenant is responsiuble for all repairs, taxes
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Greg Barrett-wilt, phd UW Mass spectrometry/proteomics facility
Central components of a mass spectrometer
Ionization source Mass analyzer/filter Detector Vacuum chamber To perform mass spectrometry two things are required: 1. The analyte must be an ion 2. The analyte must be in the gas phase
Ionization methods
Electron impact ionization (EI)
"Hard" ionization method – ionization and fragmentation occur simultaneously Incompatible with liquid streams Widely used with gas chromatography Very standardized method: 70eV electron energy NIST database
Electron impact ionization (EI)
Wiley Registry of 638,000 compounds (>700,000 spectra) NIST Library: 242,477 compounds Fred McLafferty
Chemical ionization (CI)
"Softer" ionization method – observation of intact molecular ions possible EI source filled with gas (N2, methane, ammonia) yields ionized gas molecules Reagent gas ions ionize analytes Primary ion formation CH4 + e- → CH4+ + 2e- Secondary reagent ions CH4 + CH4+ → CH5+ + CH3 CH4 + CH3+ → C2H5+ + H2 Product ion formation M + CH5+ → CH4 + [M+H]+ (protonation) [M+H]+
matrix assisted laser desorption ionization (MALDI )
"Soft" ionization method: preserves the intact analyte UV absorbing matrix is energized by the laser Matrix molecules desorbed from the surface carry analyte molecules into the gas phase Proton transfer from matrix (acid) to analyte in the gas phase UV laser used to generate ions: N2 (337 nm) or Nd:YAG (355nm) Yields singly-charged species almost exclusively Koichi Tanaka: Nobel Prize in Chemistry (2002)
maldi MALDI matrices α-cyano 4 hydroxycinnamic acid (CHCA) dihydroxy benzoic acid (DHB) 3-hydroxy picolinic acid (3-HPA) sinapic acid Analyte samples are co-crystalized with matrix molecules Example from AB Sciex 4800 TOF-TOF spotted with CHCA
MALDI BSA protein standard
Couples well to high mass-range instruments (TOF) because high molecular weight biomolecules with a single charge will be observed at high m/z. BSA protein standard [M+H]+ [M+2H]2+ [2M+H]+ [3M+H]+ [4M+H]+ [3M+2H]2+
Electrospray ionization (ESI)
"Soft" ionization that yields intact analyte ions with one or more charges A high voltage (kV) is applied to a liquid stream Flow rates can vary between ~50nL/min and 1mL/min These various flow rates require substantially different source parameters John Fenn: Nobel Prize in Chemistry (2002)
Electrospray ionization (ESI)
Couples easily to HPLC Analyte molecules can accept multiple charges (protons) Analytes can be present in multiple charge states 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 m/z 100 Relative Abundance Peptide DRVYIHPFHL (MW= ) +2 +4 +3 +33 +32 +31 Protein MW=31,928 +30 +29 +28
Mass analyzers
Quadrupole 𝑎𝑢= 8𝑒𝑈 𝑚𝑟02Ω2 𝑞𝑢= 4𝑒𝑉 𝑚𝑟02Ω2 (a) (q) U = DC voltage
𝑎𝑢= 8𝑒𝑈 𝑚𝑟02Ω2 𝑞𝑢= 4𝑒𝑉 𝑚𝑟02Ω2 U = DC voltage V = AC voltage r0 = radius Ω = AC frequency (a) (q) Randall Pedder "Practical Quadrupole Theory: Graphical Theory (2010) (Presented as ASMS poster 2001) Ubiquitous mass filters for numerous applications
Quadrupole Implications for proteomics:
Mass range: ~2000 Resolution: "unit" (100 at m/z 100, 1000 at m/z 1000) Sensitivity: moderate-low (scanning instrument) Spectral acquisition rate: moderate (~1s per spectrum) Implications for proteomics: Full MS only, low resolution, slow speed
Tandem mass spectrometer: two stages of mass filtering (MS/MS)
Triple-Quadrupole Tandem mass spectrometer: two stages of mass filtering (MS/MS) Collision cell between two quadrupole mass filters: Collision induced dissociation (CID) Additional experiments possible with a triple quadrupole SRM/MRM Domon and Aebersold, Science 312 (2006)
Triple-Quadrupole Agilent 6495 (2014) AB Sciex 6500 (2012)
Triple quadrupole instruments are a very active field of mass spec development. Considered the most widely-used type of mass spectrometer. Agilent 6495 (2014) AB Sciex 6500 (2012) Thermo TSQ Quantiva (2013)
Triple-Quadrupole Implications for proteomics:
General operating specifications same as single-quadrupole Mass range: ~2000 Resolution: "unit" (100 at m/z 100, 1000 at m/z 1000) Sensitivity: moderate-low (scanning instrument) Spectral acquisition rate: moderate (~1s per spectrum) BUT in SRM/MRM mode: Mass range: N/A Resolution: "unit" (~1 amu mass window) Sensitivity: very high (fixed mass, no scanning) Acquisition rage: N/A (~10ms per transition) Implications for proteomics: Very high sensitivity in SRM/MRM mode, very linear instrument response, excellent quantitation
3D ion trap 3D ion trap introduced commercially in 1983
Substantial increase in full scan sensitivity because all m/z ions are trapped and detected (as opposed to quadrupole instruments). Very efficient MS/MS, especially for peptides. Can only trap a limited number of ions at one time. 𝑞𝑧= 4𝑒𝑉 𝑚𝑟02Ω2 Wolfgang Paul and Hans Dehmelt: Nobel Prize in Physics (1989) V= ring electrode voltage amplitude Ω= ring electrode RF frequency r0= trap radius
2D ion trap (linear ion trap)
"Can only trap a limited number of ions at one time." 3D ion trap: ~1000 ions in a spherical volume 2D ion trap: ~30,000 ions in a cylindical volume Thermo LTQ: 2D trap replaces a 3D ion trap (single mass filter) OR 2D trap incorporated in a hybrid MS (see below) AB Sciex QTRAP: 2D trap replaces Q3 of a triple quad (tandem mass filters) Implications for proteomics: Low resolution, very good peptide MS/MS
Orbitrap Originally described in 1920 (Kingdon trap) it was only in the late '90's that it was developed into a mass spectrometer (Makarov, Anal. Chem. 2000). First new mass filter since the quadrupole ion trap in 1984 Technology owned by ThermoFisher Commercial instrument introduced in 2005 Resolving power = >200,000 𝜔𝑧= 𝑘 𝑚/𝑞 m/z analysis property:
LTQ Orbitrap Operation Principle
1. Ions are stored in the Linear Trap 2. …. are axially ejected 3. …. and trapped in the C-trap 4. …. they are squeezed into a small cloud and injected into the Orbitrap 5. …. where they are electrostatically trapped, while rotating around the central electrode and performing axial oscillation The oscillating ions induce an image current into the two outer halves of the orbitrap, which can be detected using a differential amplifier Ions of only one mass generate a sine wave signal
Frequencies and Masses
The axial oscillation frequency follows the formula Where w = oscillation frequency k = instrumental constant m/z = …. well, we have seen this before Many ions in the Orbitrap generate a complex signal whose frequencies are determined using a Fourier Transformation
Orbitrap Critical feature of Orbitrap hybrid instrument:
Multiple ion traps means that ions can be analyzed simultaneously in the different analyzers. R = 120,000 Significant increase in duty cycle (neither mass analyzer is ever idling) Implications for proteomics: High resolution/mass accuracy (Orbitrap), very good peptide MS/MS (ion trap), very high duty cycle (hybrid), fast scan speed
Orbitrap XL: LC/MSn ~1000 proteins in 4 hours 40 60 80 100 120 140 160
180 200 220 240 260 280 Time (min) 10 20 30 50 70 90 Relative Abundance ~1000 proteins in 4 hours
Several instrument types
Orbitrap Elite (2010) Q Exactive (~2008) LTQ Orbitrap XL (~2007) Orbitrap Fusion (2013)
Time-of-flight (TOF) 𝑡=𝑘 𝑚 𝑞
𝑡=𝑘 𝑚 𝑞 Where k incorporates accelerating voltage and distance of flight tube
Time-of-flight: esi
Time-of-flight: maldi
AB Sciex 4800 MALDI TOF-TOF: MS and MS/MS
Q-tof (or Qq-TOF) Similar to triple-quad MS, but the third quad is a TOF High resolution, accurate mass MS/MS is performed after MS survey scan (in series) by increasing the offset energy in the collision cell High-resolution MS1 and MS/MS Assist in database searching of proteomic data, unknown i.d.
Ion mobility MS Agilent 6560
Adds a drift tube in the flight path of the ions prior to the TOF region This allows for separation of species based on their gas-phase cross-section: Small ions are retarded by gas molecules less than large ions (even for same m/z) Waters
TOF features MALDI TOF: Decoupled from HPLC: fast (~1 sec/spectrum)
MS/MS capable (TOF/TOF): ideal for rapid protein ID from 1D gel band Resolution > 20,000 Laser rate 1kHz-2kHz (new instruments) ESI-TOF: Resolution >40,000 Fast electronics give excellent sampling across chromatographic peaks Q-TOF: High resolution MS1 and MS/MS Ion Mobility Q-TOF: Additional structural information from cross-section (separate isobarics) Non-covalent interaction experiments Implications for proteomics: High resolution/accurate mass for MS and MS/MS, fast scan speed, wide mass range (100,000 MS1, 4000 MS/MS)
Wrap-up Ionization sources: EI CI MALDI ESI Mass Analyzers:
Single and triple quadrupole 3D and 2D ion trap Orbitrap Time-of-flight (TOF/TOF) Q-TOF Ion mobility Others: APCI APPI ICP SIMS Others: Magnetic sector Isotope ratio FT-ICR Accelerator
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