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|By Business Wire
Article Rating:
|November 8, 2012 12:00 PM EST
Airpush (www.airpush.com), winner of the "Best Mobile Ad Network" at the 2012 MEAs, today announced at the SF AppShow a partnership with Appthority, the Authority in App Reputation™, designed to target the proliferation of mobile malware. This partnership will integrate Appthority's industry-leading mobile security technology directly into the Airpush platform in order to scan all advertiser app promotions and URLs as an additional layer of protection to Airpush's manual approval process. Airpush seeks to set a new standard in mobile ad network security practices and encourages other networks to follow suit.
Mobile malware is one of the most severe and prevalent threats to the mobile ecosystem. Innovative security companies like Appthority are rapidly developing solutions to combat this threat, but without scanning at the distribution level, they can only eliminate a small percentage of malicious threats. By integrating Appthority's technology directly into its platform, Airpush has virtually eliminated the threat of mobile malware distribution via its network of over 50,000 applications.
"The threat of mobile malware is damaging to all players in the ecosystem, and mobile ad networks are uniquely positioned to combat the threat," said Asher Delug, Founder and CEO of Airpush. "After evaluating a wide variety of mobile security platforms, the strength and speed of Appthority's technology made them the clear choice. Since we are scanning thousands of advertiser campaigns on a regular basis, we required a technology partner with a fast and robust API. Appthority fit the bill."
According to Airpush data, the vast majority of mobile malware advertisers are based in Russia and Eastern Europe, with China and other Asian countries close behind. Recently, however, there has been a rise in malware advertisers within certain Western European markets. Appthority's technology enables Airpush to systematically monitor threats on a global basis and remove those threats in near real-time.
"Mobile malware has been a thorn in the side of the thriving app ecosystem," said Kevin Watkins, CTO and co-founder of Appthority. "We are proud to assist Airpush with the technology that improves the state of security across the app world. Airpush is being proactive by attacking the malware problem at its source – distribution – and that demonstrates their commitment to protecting their customers."
About Airpush
Named "Best Mobile Ad Network" at the 2012 Mobile Excellence Awards, Airpush is on a mission to redefine mobile advertising for developers and advertisers. Over 50,000 apps and 2,000 advertisers rely on Airpush to deliver the industry's highest performance, driven by exceptional ad formats and targeting technology. Founded in 2011 by mobile advertising veterans, the company has approximately 100 employees and offices in Los Angeles and Bangalore.
For more information visit Airpush.com or follow us on Twitter @AirpushAds.
About Appthority
Appthority - The Authority in App Security™ helps the enterprise identify and manage the risks hidden in mobile apps. The cloud-based Appthority Platform™ automatically identifies and grades risky behavior in mobile apps including known and unknown malware, new malware used in targeted attacks, corporate data exfiltration, and intellectual property exposure. For more information on Appthority please visit www.appthority.com or follow on Twitter: @GetAppthority
NHK, Japan Broadcasting, will feature the upcoming @ThingsExpo Silicon Valley in a special 'Internet of Things' and smart technology documentary that will be filmed on the expo floor between November 3 to 5, 2015, in Santa Clara. NHK is the sole public TV network in Japan equivalent to the BBC in the UK and the largest in Asia with many award-winning science and technology programs. Japanese TV is producing a documentary about IoT and Smart technology and will be covering @ThingsExpo Silicon Val...
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Building a cross-cloud operational model can be a daunting task. Per-cloud silos are not the answer, but neither is a fully generic abstraction plane that strips out capabilities unique to a particular provider. In his session at 20th Cloud Expo, Chris Wolf, VP & Chief Technology Officer, Global Field & Industry at VMware, will discuss how successful organizations approach cloud operations and management, with insights into where operations should be centralized and when it's best to decentraliz...
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SYS-CON Events announced today that Juniper Networks (NYSE: JNPR), an industry leader in automated, scalable and secure networks, will exhibit at SYS-CON's 20th International Cloud Expo®, which will take place on June 6-8, 2017, at the Javits Center in New York City, NY. Juniper Networks challenges the status quo with products, solutions and services that transform the economics of networking. The company co-innovates with customers and partners to deliver automated, scalable and secure network...
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@DevOpsSummit at Cloud taking place June 6-8, 2017, at Javits Center, New York City, is co-located with the 20th International Cloud Expo and will feature technical sessions from a rock star conference faculty and the leading industry players in the world. The widespread success of cloud computing is driving the DevOps revolution in enterprise IT. Now as never before, development teams must communicate and collaborate in a dynamic, 24/7/365 environment. There is no time to wait for long developm...
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Grape Up is a software company, specialized in cloud native application development and professional services related to Cloud Foundry PaaS. With five expert teams that operate in various sectors of the market across the USA and Europe, we work with a variety of customers from emerging startups to Fortune 1000 companies.
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Financial Technology has become a topic of intense interest throughout the cloud developer and enterprise IT communities. Accordingly, attendees at the upcoming 20th Cloud Expo at the Javits Center in New York, June 6-8, 2017, will find fresh new content in a new track called FinTech.
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In his keynote at 19th Cloud Expo, Sheng Liang, co-founder and CEO of Rancher Labs, discussed the technological advances and new business opportunities created by the rapid adoption of containers. With the success of Amazon Web Services (AWS) and various open source technologies used to build private clouds, cloud computing has become an essential component of IT strategy. However, users continue to face challenges in implementing clouds, as older technologies evolve and newer ones like Docker c...
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Cloud Expo, Inc. has announced today that Aruna Ravichandran, vice president of DevOps Product and Solutions Marketing at CA Technologies, has been named co-conference chair of DevOps at Cloud Expo 2017. The @DevOpsSummit at Cloud Expo New York will take place on June 6-8, 2017, at the Javits Center in New York City, New York, and @DevOpsSummit at Cloud Expo Silicon Valley will take place Oct. 31-Nov. 2, 2017, at the Santa Clara Convention Center in Santa Clara, CA.
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The 20th International Cloud Expo has announced that its Call for Papers is open. Cloud Expo, to be held June 6-8, 2017, at the Javits Center in New York City, brings together Cloud Computing, Big Data, Internet of Things, DevOps, Containers, Microservices and WebRTC to one location. With cloud computing driving a higher percentage of enterprise IT budgets every year, it becomes increasingly important to plant your flag in this fast-expanding business opportunity. Submit your speaking proposal ...
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In recent years, containers have taken the world by storm. Companies of all sizes and industries have realized the massive benefits of containers, such as unprecedented mobility, higher hardware utilization, and increased flexibility and agility; however, many containers today are non-persistent. Containers without persistence miss out on many benefits, and in many cases simply pass the responsibility of persistence onto other infrastructure, adding additional complexity.
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Apr. 26, 2017 05:15 PM EDT Reads: 2,780

subset: commoncrawl


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Mallory Hagan - The Point Honors Gala hosted by the Point Foundation - Arrivals at New Public Library 5th Avenue - New York, United States - Monday 11th April 2016
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Mallory Hagan - Celebrities attend the Zooey Deschanel for Tommy Hilfiger Collection launch event at The London Hotel. - Los Angeles, California, United States - Wednesday 9th April 2014
Tom Murrow, Veronica Kelly, Greg Kelly, Mallory Hagan and James Kelly - Voice of September 11th Always Remember Gala - Inside Arrivals - New York City, NY, United States - Thursday 17th October 2013
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Jason Drucker is Greg Heffley in the upcoming movie based on the novel of the same name.
Ford attributes his career success to films that pass 'from generation to generation'.
Kurt Russell and Goldie Hawn got walked in on by police on their first night together.


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Personal Statement
I'm dedicated to providing optimal health care in a relaxed environment where I treat every patients as if they were my own family....more
I'm dedicated to providing optimal health care in a relaxed environment where I treat every patients as if they were my own family.
More about Dr. M.g.k. Reddy
Dr. M.g.k. Reddy is one of the best Homeopaths in Yelahanka, Bangalore. You can meet Dr. M.g.k. Reddy personally at German Dispensary in Yelahanka, Bangalore. You can book an instant appointment online with Dr. M.g.k. Reddy on Lybrate.com.
Find numerous Homeopaths in India from the comfort of your home on Lybrate.com. You will find Homeopaths with more than 41 years of experience on Lybrate.com. You can find Homeopaths online in Bangalore and from across India. View the profile of medical specialists and their reviews from other patients to make an informed decision.
Find numerous Homeopaths in India from the comfort of your home on Lybrate.com. You will find Homeopaths with more than 41 years of experience on Lybrate.com. You can find Homeopaths online in Bangalore and from across India. View the profile of medical specialists and their reviews from other patients to make an informed decision.
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20 years old male from Patiala asked
I am suffering from ring worms. I have tried many medicines. Can you please tell me some good medicine to get rid of ring worm.
Homeopath, Faridabad
Hello, Ringworm is a contagious itching skin disease occurring in small circular patches, caused by any of a number of fungi. Take a single dose of tuberculinum 200ch, 5 drops, from the next day take tellurium 30, 5 drops once daily. Apply topi azadirachta ointment. Revert after 7 days.
20 years old male from Anantapur asked
Hi I am working one MNC company there is every month one week night duty it is about 8 hours 11: 30 to 7: 00 and I am sleeping morning times during that duty times about 5 or 6 hours so any problem for that?
BHMS, MD - Alternate Medicine
Homeopath, Thrissur
A continuous sleep for 7-8 hrs in night is good for health. Our body bio-rhythm maintain with the 3 main natural process called Sleep, good appetite, normal bowel movements. Health balanced on these rhythm We get dizziness next day if we not slept well previous night. This is indisposition from health. It is because the repair process is pending in our body. No way other than sleep, should get another sleep to recover. But as we on with our duties body will adjust for certain period. Body adaptability. For you, if 1 week night duty in a month makes any health problems, should be monitored and corrected. A continuous sleeping irregularity leads to sick to disease.
24 years old male asked
How control masturbate. And also m feeling lyk I want to be naked in front of women . Y we feel lyk that.
MD - Homeopathy
Homeopath, Aurangabad
Acid phos 200 take 1 drop 3 times x 7 day above is homeopathic medicine and available in homeopathic store also safe to take and no side effects for future management
24 years old male from Siwan asked
My wife is very thin, she eat too much but it does not effect her body? What's the reason behind it and how should I make her fat. Please suggest me some tips and give me some advice.
Diploma in Obstetrics & Gynaecology, MBBS, MD - Community Medicine
Gynaecologist, Lucknow
Hello, being thin may be her body constitution. You can get her thyroid profile checked to rule out hormonal imbalance. She should take rest also along with high calorie diet.
25 years old male from Bangalore asked
I am 25 yr old and my weight is 58 kg from four yrs, I take healthy foods with fruits still my weight is not increasing my height is 5'7. What I need to do?
Advanced Aesthetics
Ayurveda, Gulbarga
? Several people weigh less than their ideal body weight ? The condition is referred to as being underweight Symptoms to look for: ? Being underweight can lead to: o Bone loss o Heart problems o Infertility o Lack of nutrition o Anaemia o Weak immune system o Frequent bouts of illness Causes: ? Excessive physical activity ? Skipping meals ? Fasting ? Emotional stress ? Genetic factors ? Hyper-active thyroid leading to frequent bowel movement ? Inability to absorb food causing loss of fat Natural home remedy using ashwagandha, hot milk and clarified butter: 1. Take 1 glass hot milk 2. Add 2 tbsp ashwagandha powder, commonly available ayurvedic herb 3. Add 1 tsp clarified butter 4. Mix well 5. Drink twice every day 6. Do this for 1 month For weight gain 100% safe and natural medicines available, in 1 month 4-5 kg increases without any side effects for medicines consult privately and trial pack for 1month ..and only 3 month course ..
22 years old female from Lucknow asked
Dr. I have a complaint of breast size problem actually my breast are too small 30 cm all teases me pls advice me how to make my breast size big?
Homeopath, Raebareli
Dear user you need to increase your protein intake as well fats intake in your diet. Apart also a stress free life. With above use a remedy Alfalfa Q 20 drops in half cup normal water once daily before meal. And recheck your size after 3 weeks.
22 years old male from Kochi asked
I am a 22 year old male but for the past 2 weeks I am facing some problems like lack of sleep and irregular heartbeats and I am undergoing through the toughest situations of my life, what should I do to make myself better?
Diploma in Obstetrics & Gynaecology, MBBS
General Physician, Delhi
You are passing through stress which is bad for heart. Without being preachy, I suggest you turn stress into an opportunity to hope for good results which will work like good cholesterol to help the heart. Get investigation done for heart like ecg and halter tests plus blood pressure and blood tests done for sugar and lipids. Regarding lack of sleep, do not take any medicines, depend upon foods like milks and curds that help get sleep besides giving get basic proteins. Get back here with reports. You will get more guidance from any of us here on the forum.
Shared 2 years ago • Featured Tip
General Physician, Delhi
If your blood pH were to vary 1 or 2 points in either direction, it would change the electrical chemistry in your body, there would be no electrical power and in short duration you would drop dead!
Answers to the following 3 questions will tell you why your body's pH balancing regulates your health and life-
Q1) What is pH?
- pH is a measure of how acidic or alkaline something is, and our blood pH must be slightly alkaline for us to keep healthy. The scale ranges from 1 to 14 with 1 being very acid, 7 neutral and 14 very alkaline. The ideal pH level for your blood is around 7.35 and we help our bodies to maintain this pH balance by eating more alkaline-forming foods and fewer acid-forming foods.
Q2) How does your body strike a balance?
- When we consume acid-forming foods, our body works to bring our blood pH back into balance by releasing alkaline-rich minerals into our bloodstream (e.g. calcium, phosphorus and magnesium).
- If we don't eat enough alkaline-generating foods, then our body pulls these minerals from our bones, teeth and other organs. This can be a risk to our immune system making us weaker.
Q3) What should you eat?
Eat a diet that consists of 60-80% alkaline-forming foods and about 20-40% acid-forming foods.
- Alkaline-forming foods: Most fruits, vegetables, herbs, nuts, seeds, and herbal teas.
- Acid-forming foods: Most grains, beans, meats, dairy products, fish, fast foods, and processed foods.
Have any further questions? Click on consult privately to ask me.
From Lybrate: If you found this tip useful, please thank the doctor by clicking on the heart icon below. Also, spread good health by sharing this tip with your loved ones over WhatsApp, Facebook and other media.
Answers to the following 3 questions will tell you why your body's pH balancing regulates your health and life-
Q1) What is pH?
- pH is a measure of how acidic or alkaline something is, and our blood pH must be slightly alkaline for us to keep healthy. The scale ranges from 1 to 14 with 1 being very acid, 7 neutral and 14 very alkaline. The ideal pH level for your blood is around 7.35 and we help our bodies to maintain this pH balance by eating more alkaline-forming foods and fewer acid-forming foods.
Q2) How does your body strike a balance?
- When we consume acid-forming foods, our body works to bring our blood pH back into balance by releasing alkaline-rich minerals into our bloodstream (e.g. calcium, phosphorus and magnesium).
- If we don't eat enough alkaline-generating foods, then our body pulls these minerals from our bones, teeth and other organs. This can be a risk to our immune system making us weaker.
Q3) What should you eat?
Eat a diet that consists of 60-80% alkaline-forming foods and about 20-40% acid-forming foods.
- Alkaline-forming foods: Most fruits, vegetables, herbs, nuts, seeds, and herbal teas.
- Acid-forming foods: Most grains, beans, meats, dairy products, fish, fast foods, and processed foods.
Have any further questions? Click on consult privately to ask me.
From Lybrate: If you found this tip useful, please thank the doctor by clicking on the heart icon below. Also, spread good health by sharing this tip with your loved ones over WhatsApp, Facebook and other media.
21 years old male asked
My body is becoming severe hot when the evening starts daily. What was my problem and what is the solution.
MBBS, MD - Internal Medicine
Internal Medicine Specialist, Faridabad
it may be due to stress of work. or thyroid or any infection for mild fever. pl. check up your thyroid profile, vit.D., vitB12. cbc. till then you can take tab. dolo 500 mg sos. cap. zevit 1od in morning.
1 year old female from Chennai asked
General Physician, Mumbai
Fever with body pain and joint pain are initial symptoms of dengue and to be confirmed by a blood test
20 years old male from Kanpur asked
General Physician, Cuttack
1. Take one tablet of crocin advance as and when required upto a maximum of three tablets daily after food 2. Cold sponging sos 3. Drink plenty of water 4. Take rest 5. If no relief do blood examination like esr, cbc, widal/ typhidot test, blood for mp/malaria antigen, dengue antigen (ns1) test, urine re/me, x ray chest after consulting physician and follow the advice and treatment.
21 years old male asked
Hello, there can be many reasons behind it like mental worries, nutritional deficiency, hereditary and anaemia. *Make a habit of 8-10 glasses of water daily. *Eat healthy and balanced food like pulses, green leafy vegetables, fresh fruits and nuts etc. *Eggs as it is loaded with essential nutrients such as proteins, vitamin b12, iron, zinc and omega 6 fatty acids in large amount. *Add more vitamin rich food to your diet. *Avoid stress and anger. *Cut down the use of hair spray, hair gel. *Take a sound sleep. Take homoeopathic medicine Lycopodium 30 (4 drops in little water) twice a day for 15 days and update Apply jobarandi hair oil.
Shared 2 years ago • Featured Tip
Dentist, Ernakulam
It is always better to visit a general dentist first to get an impartial view about your problems and prescribe a best suited treatment for you.
year old male from Bangalore asked
Doctor .I want to increase my penis size. And time also. Bcz I am able to do sex. Only 2 mint. It s not satisfied. So please suggest me what should I do. How can increase time and size.
Bachelor of Ayurveda, Medicine and Surgery (BAMS)
Sexologist, Jodhpur
Do not worry about size as far as you get good erection and timngs. Women vagina initial 2 inches is sensitive so size aythg bigger than 2 inch is sufficient. Imprtant is good erection and timings. Consult for detailed discssion and solve your erection and timing issues. Pe is common problem and can be easily cured with proper guidance and ayurvedic medicines. Some of the common cause is excessive masterbation, over excitement, harmonal imbalance, side effects of some medicines etc. Consult to discuss your problem in details. We will educate you on sex, sexual issues its causes. Solution. Do not worry once treatment starts you will see results within 10 days.
21 years old male from Lucknow asked
My weight is reduced from previous months & keep on reducing. What supplement should I use to regain my body weight.
Dietitian/Nutritionist, Chandigarh
Hi lybrate-user never take supplements here are 6 ways to gain weight healthily: 1. Eating 5 to 6 meals a day in order to gain weight, start eating 5-6 meals a day by breaking them into parts. Eating a lot at once may cause indigestion if the body fails to absorb all nutrients. Eat in small amounts, even if you are not hungry and there will be an increase in appetite from day to day. The aim should be to have a generously portioned breakfast, lunch and dinner, as well as two to three snacks in between. 2. Start weight training if you build your muscles through weight training, it will convert the extra weight into lean body mass and also stimulate your appetite. The extra muscle increases the rate of metabolism. When you start exercising you will often experience extreme muscle soreness but it goes away in 3-5 days. 3. Load up on protein besides weight training, a protein-rich diet can help you gain mass. Eating a meal heavy in protein shortly after finishing exercise can be highly effective in adding extra pounds to your body. However, a diet focused only on lean protein is not helpful. It is important to make sure that your diet has plenty of fat in it. Eat foods rich in protein like cheese, meat, eggs, etc to gain weight in a healthy manner. 4. Workout your entire body a lot of people focus only on training the muscles on their arms, chest and shoulders and this is a grave error. Firstly, it will give you a disproportionate body; and it will make you prone to injuries. Therefore, it's important to work out the entire body for a complete and balanced look. 5. Drink your calories to reach the calorie requirement only by eating may be tough. Drinking milk, milkshakes or protein shakes with little or no sugar can be an effective way to load up on the calorie requirements. 6. Be patient and believe in yourself gaining weight quickly in a healthy manner is unrealistic. Every body type is different and it takes months to gain weight. Living healthy by eating the right diet will increase your strength. It is also important to keep following the routine even if you don't see any gains. Caution: junk food and fried foods must be avoided; although have a tendency to add more fat to your body but are very low in vitamins and minerals which are essentially required to keep you really fit. Related tip: the most powerful way to put on weight - snacking right.
33 years old male from Jodhpur asked
My sugar level is high and few day ago I got injured badly. This injury pain badly what I should do ?
General Physician,
You will need pain relieving medicines and sugar control. For persoanlised care you ask me privately.
26 years old male from Hyderabad asked
I have pain in left hand &left leg since 8 months when I sit on floor after 10 mints leg begins heavier.
Hello, you can take homoeopathic medicine Rhus Tox 30 ( 4 drops in little water) thrice a day for a week and revert.
year old male from Yavatmal asked
I am 26 year old male. I am suffering from Depression by thinking about the past incidents/events which happened to me 3-4 years ago .I do my Yoga ,pranayama and meditation regularly but still at times I get depressed by thinking about the past incidents that happened to me ,and I end up blaming ,cursing and hating some people in my past for ruining my Life or whom I consider responsible for bad incidents that happened to me ,I have developed hatred for those people .I regret a lot about the past and mostly dwell in the past also at times I curse God about the past. I also at times find it tough to accept the present situation and reality and I always question a lot that why these things happened to me ? Also I consider myself Unlucky that those incidents happened to me. I find it difficult to live in the present and happily and I can not completely concentrate and focus on my work .And I feel I was a victim and injustice happened to me .How should I get out of this thought cycle and depression ?
Reparenting Technique, BA, BEd
Psychologist, Bangalore
The answer to your question is quite obvious but still I think it would be very useful to meet with a counselor and purge the past out of your system by talking to that person. By delving into the past it is affecting your present and future. Also, the danger I am concerned about it that you may find yourself in self-pity a lot of the time and that is extremely bad for you. You cannot change the past but you can make your present and future far better than what it is now by closing the past. Counseling will certainly help tremendously. I suspect that you are one of many who have been taking medicines but not working with a counselor in tandem. You need both counseling and medical intervention. The combination is ideal for the cure to be complete. In fact, counseling will need to go on for a long time to develop skills and techniques to cope with everyday challenges of your life. If you remain episode-free for three years at least, you can be weaned off of the medication. So I assume that for all of these many years you have been letting the medicine do all the work on your depression and very little effort from your side. This will not do at all. So please start counseling immediately. In the meantime please do the following that can be done all by yourself as you desire: You must be active and work towards your reformation. Many lifestyle changes need to be made to progress away for the condition. When you are depressed, you will feel lazy though I would like to call it lethargy. There is an inertia and lack of energy to even think, let alone act. You are required to literally do the opposite of what you feel like doing. Depression makes you want to withdraw, lie down, sleep, become lethargic, slow down, make you too lazy to even think etc. So when I suggested doing the opposite, you will need to fight this disease. You must become active; stay upright during the daylight time; meet people; never sleep during the day, wake up by 6 am every day, play some active games, especially contact games, do physical exercises, talk to people and join some social clubs, eat more of proteins and vegetables, attend Yoga classes etc. Watch sitcoms on TV or comedies and cheer yourself up. Go for excursions in groups, for outings, camps, conferences, and religious conventions. Get a pet dog and spend time training it, exercising it and relating to it. Expose yourself to some sunlight every day, at least 30 minutes but not in the scorching heat. Whatever happens, please incorporate these three important adaptations in your life: always be responsible, be respectful, and be functional. If you did these three things, lots of things will go well in life. Please pray and have faith in God to alleviate your sufferings. Don't wait for others to help. Use your own motivation, which might be at its lowest, but persevere and win this battle. If you are young and you can do it with some commitment. The doctor and counselor are there only to facilitate you, all the hard word must come from you, and your cooperation with them is very critical for your success.
20 years old female from Visakhapatnam asked
I have skin problems. There are large pores around my both the cheeks. I'm 20 years old and I look much older than my age. Would you suggest any medication.
Bachelor of Ayurveda, Medicine and Surgery (BAMS)
Ayurveda, Bundi
Large pores need skin cleaning every day with proper care and non chemicals. Second iswash face with neem water every day. Third thing is egg pack should be used (no yellow, only white), then use Varnyadi gana herb combos of 10 herbs for few weeks as directed. Keep away from dust by proper care.
39 years old male from Delhi asked
What causes Hashimoto's thyroiditis, and what is the best method of treatment? Can iodine help this condition?
MBBS, CCEBDM, Diploma in Diabetology
Endocrinologist, Hubli-Dharwad
Mr. Lybrate-user, hashimoto's thyroiditis (ht) is an autoimmune disease, with lymphatic infiltration of thyroid gland. It becomes firm or globular. There are anti bodies produced against thyroid peroxidase enzyme (anti tpo ab) which destroy thyroxine secreting cells resulting in hypothyroidism. Treatment is to give thyroxine tablets. Iodine has no role in the treatment. In fact one of the causes is supposed to be taking extra iodine through iodinated salt.
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A series of interconnecting or adjacent cities with a total population of 10 million or greater. Due to urban and transportation development between the individual cities, the boundries of the areas tend to become blurred, making it seem like one massively overdeveloped region. Many of these areas, as well as being centers of business and industry, are also a cultural meccae and have a major affect on the cultures and lifestyles of their residents as well as their countries and the rest of the world and vice versa.
The American East Coast Megalopolis, which includes: Boston; New York City; Philidelphia; Baltimore; Washington D.C.
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I've been taking photos since I was a kid, which turns out to be over a half century now. I live in Kalamazoo, Michigan and show and sell my work here. I shoot most anything, but I have special relationship with the moon.
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Prion disease incubation time in mice is determined by many factors including genetic background. The prion gene itself plays a major role in incubation time; however, other genes are also known to be important. Whilst quantitative trait loci (QTL) studies have identified multiple loci across the genome, these regions are often large, and with the exception of Hectd2 on Mmu19, no quantitative trait genes or nucleotides for prion disease incubation time have been demonstrated. In this study, we use the Northport heterogeneous stock of mice to reduce the size of a previously identified QTL on Mmu15 from approximately 25 to 1.2 cM. We further characterised the genes in this region and identify Cpne8, a member of the copine family, as the most promising candidate gene. We also show that Cpne8 mRNA is upregulated at the terminal stage of disease, supporting a role in prion disease. Applying these techniques to other loci will facilitate the identification of key pathways in prion disease pathogenesis.
The online version of this article (doi:10.1007/s10048-009-0219-8) contains supplementary material, which is available to authorized users.
Transmissible spongiform encephalopathies or prion diseases are progressive neurodegenerative disorders for which there is no effective treatment and are invariably fatal. They occur in several mammalian species including scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle and Creutzfeldt–Jakob disease in humans. Although there is variation between disease types, they are characterised by a clinically silent long incubation period and share the neuropathological features of neuronal loss, spongiform change (vacuolation) and deposition of an abnormal form of the prion protein. Prion diseases are naturally and experimentally transmissible, including to laboratory mice.
Experimental transmission of prions to different strains of inbred mice shows highly reproducible variation in incubation time, reflecting a strong genetic influence [1–4]. The similarities between the mouse and human genome, and conservation of these diseases across mammalian species, suggest that the identification of quantitative trait genes in mice will not only highlight common disease pathways and new therapeutic targets but will also identify susceptibility genes in human. The main genetic determinant of incubation time and susceptibility in both mouse and humans is variation in the prion gene (Prnp) itself. In mice, Prnpa (Leu-108, Thr-189) and Prnpb (Phe-108, Val-189) mice have short and long incubation times, respectively [4, 5]. However, prion transmissions to different Prnpa allele mice show significant variation in incubation time, suggesting that other genes are also important [3, 4, 6]. Quantitative trait studies using a variety of mouse crosses and prion strains have successfully identified several loci across the genome [7–11]; however, the underlying quantitative trait genes (QTG) for these regions have not yet been identified. In these crosses, no quantitative trait locus (QTL) for prion disease incubation time was detected on Mmu19; however, using a heterogenous stock of mice, we previously identified Hectd2 as a QTG [12]. In a C57BL/FaDk × RIII/FaDk backcross inoculated intracerebrally with BSE prions, Manolakou et al. [10] identified a QTL of significant linkage on Mmu15. The two-way cross resulted in low-resolution mapping such that the interval spans approximately 25 cM and is therefore too large for individual candidate gene analysis. Several strategies are available for fine mapping including the use of heterogeneous stocks (HS) of mice [13–15]. The Northport HS was produced from semi-randomly mating eight different parental strains (A/J, AKR/J, BALB/cJ, CBA/J, C3H/HeJ, C57BL/6J, DBA/2J and LP/J) over multiple generations [16]. This breeding scheme results in offspring with chromosomes that contain multiple recombinations, modelling an outbred population with the advantage that all the parental alleles are known. In combination with the development of appropriate multipoint linkage programmes, HS populations have successfully been used to obtain mapping resolution of 1–2 cM [16–19]. In this study, we use the Northport HS to refine the region of linkage on Mmu15 and identify Cpne8 as a potential quantitative trait gene for prion disease incubation time.
RIIIS/J mice were obtained from the Jackson Laboratory (Bar Harbor, Maine, USA), and all other inbred lines were obtained from Harlan, UK. Twenty-eight pairs of Northport HS mice were obtained from R. Hitzemann (Portland, Oregon, USA) at generation 35. The offspring were mated semi-randomly, avoiding shared grandparents, to obtain 49 mating pairs. Approximately 1,000 offspring (generation 37) were used for inoculation. Mice were anaesthetised with isofluorane/O2 and inoculated intracerebrally into the right parietal lobe with 30 μl Chandler/RML prions as previously described [7]. Incubation time was calculated retrospectively after a definite diagnosis of prion disease had been made and defined as the number of days from inoculation to the onset of clinical signs [20]. All procedures were conducted in accordance with institutional and UK regulations on animal welfare.
DNA was extracted from 0.5 cm tail biopsies using a Promega DNA extraction kit and resuspended in 50 μl TE (10 mM Tris–HCL, 1 mM EDTA, pH 7.5). A 1:10 dilution of this stock was used as template for subsequent PCRs. Microsatellites were selected from the UCSC Mouse Genome Browser (http://genome.ucsc.edu) and Mouse Genome Informatics web site (www.informatics.jax.org) and checked that they were polymorphic within the parental strains of the HS. Twenty microsatellite markers from Mmu15, (D15Mit234-D15Mit43 (Electronic supplementary material, Table 1), with an intermarker distance of ~1–3 cM, were genotyped in approximately 400 animals which represent the extreme 20% of both sides of the incubation time distribution. Fluorescently labelled and standard oligonucleotides were synthesised by Sigma-Genosys. PCR reactions were all carried out in 5 μl on 96-well plates using MegaMix Blue (Microzone Ltd.) according to the manufacturer's instructions using 5 pmol of each primer. PCR conditions were determined empirically, but in general, cycling conditions using a PTC-225 (MJ Research) thermal cycler were as follows: 94°C for 10 min; 94°C 30 s, 55°C 30 s, 72°C 30 s for 35 cycles; 72°C for 5 min. Products of appropriate size and fluorochrome were pooled before further processing. Reactions were ethanol-precipitated, washed in 70% ethanol and resuspended in a total of 10 μl including 5.8 μl MegaBACE loading solution (GE Healthcare) and 0.2 μl MegaBACE ET400-R size standard (GE Healthcare). Dilution (1/10) in MegaBACE loading solution was used for analysis. Fragments were heat-denatured at 94°C for 2 min before loading onto a MegaBACE1000 capillary sequencer (GE Healthcare). Samples were injected at 3 kV for 45 s and run at 10 kV for 60 min. Fragment sizes were analysed using Genetic Profiler v1.1 (GE Healthcare). Multipoint linkage analysis was carried out using HAPPY (http://www.well.ox.ac.uk/happy).
Primers and probes for genotyping SNPs (Electronic supplementary material, Table 4) were designed using criteria defined by Applied Biosystems and PrimerExpress software (Applied Biosystems). MGB probes labelled with either Vic or Fam were purchased from Applied Biosystems, and primers for amplification were obtained from Sigma-Genosys. All reactions were carried out in 5 μl (5 pmol of each primer and 1 pmol of each probe) using the Allelic Discrimination function on a 7500 Fast real-time PCR system (Applied Biosystems) using QuantiTect probe PCR kit (Qiagen). Conditions were 95°C for 5 min; 95°C 15 s, 60°C 60 s for 40 cycles.
Genomic DNA for the parental strains were obtained from the Jackson Laboratory (Bar Harbor, Maine, USA). Genomic sequence and intron/exon structure for each gene was established using the UCSC (http://genome.ucsc.edu/) and ENSEMBLE (http://www.ensembl.org/index.html) genome browsers. PCR products were designed to cover the open reading frame, 5′ and 3′ untranslated region, intron–exon boundaries and potential promoter sequences as defined by the literature for each gene or as predicted by PROSCAN. Primers were designed using Primer 3 (http://frodo.wi.mit.edu/). PCR products were generated in 25 μl as above with 10 pmol of each primer. Forty cycles were carried out as above except for the annealing and extension steps of 60°C 45 s, 72°C 60 s. PCR products were cleaned using Microclean (Microzone Ltd.) according to the manufacturer's instructions and resuspended in H2O. PCR product (100–200 ng) was added to a 15-μl sequencing reaction including 5 pmol of either the forward or reverse primer, 1 μl BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems) and 5 μl Better buffer (Microzone Ltd.). Cycling conditions were 95°C 30 s, 50°C 15 s, 60°C 120 s for 30 cycles. Reactions were ethanol-precipitated as above and resuspended in 10 μl MegaBACE loading solution. Samples were injected at 3 kV for 40 s and run at 9 kV for 100 min on a MegaBACE1000 capillary sequencer.
RNA was extracted from whole brains from either uninfected or RML terminally sick mice. Tissue was homogenised using a Ribolyser according to the manufacturer's instructions. RNA was prepared using either RNeasy Maxi (Qiagen) kit or TRIreagent (Ambion) according to the manufacturer's instructions. Samples were treated with DNaseI (Qiagen) and purified further using RNeasy Mini (Qiagen) columns according to the manufacturer's instructions. Four micrograms total RNA was reversed-transcribed with AMV reverse transcriptase and random primers from the Reverse Transcription System (Promega) according to the manufacturer's instructions. Reactions with no reverse transcription were also carried out for each sample to ensure no genomic DNA contamination. Cpne8 quantitative PCR was carried out on a 7500 Fast real-time PCR system (Applied Biosystems) in a total volume of 15 μl using 1 μl cDNA (200–300 ng) and RoxMegaMixGold (Microzone Ltd.) according to the manufacturer's instructions. Primers (6 pmol) F-5′-CGCCGTACACCCCTCCTA-3′, R-5′-GTGTGAGGGACATCAGCATCTG-3′ and probe (3 pmol) 5′-Fam-CTGCAGACGCAAATATGACCACGC-Tamra-3′ were designed using PrimerExpress software (Applied Biosystems) and supplied by Sigma Genosys. Endogenous controls (GAPDH and β-actin) were VIC-labelled and supplied as a kit by Applied Biosystems. Primers and probe (supplied by Applied Biosystems) for an additional endogenous control, Thy-1, were designed and used as above. Thy-F-5′-CAGGCACCCTTGGGATACC-3′; Thy-R-5′-TGGAACTATATCCCGACCAACCT-3′; probe 5′-Vic-ACGTACCGCTCCCGCGTCACC-Tamra-3′. Endogenous controls were duplexed with the Cpne8 reaction according to the manufacturer's instructions (GAPDH and β-actin) or for Thy-1 using the same primer and probe concentrations as for Cpne8. All reactions were carried out in triplicate using the following cycling conditions: 95°C 5 min; 95°C 15 s, 60°C 60 s for 40 cycles.
A previous study identified a 25-cM region on Mmu15 linked to BSE prion incubation time in mice [10]. To reduce this interval, we used the Northport HS of mice. The original cross used C57BL/FaDk and RIII/FaDk mice which are not present in the Northport HS; however, we reasoned that the C57BL/6J alleles present in the HS mice would be a reasonable substitute for the C57BL/FaDk allele. Primary passage of BSE prions across the species barrier from cattle to mice results in incubation times in excess of 400 days, which can be very variable and frequently results in a less than 100% attack rate. To avoid these issues, we used the Chandler/RML mouse adapted scrapie prion strain which was derived originally from goat scrapie but has been passaged multiple times in mice [21] and no longer presents a species barrier to mice. Although it is possible that the Mmu15 QTL is specific to the BSE strain of prions and might not be relevant in mouse scrapie, our ultimate goal is to identify candidate genes that play a role in the fundamental processes of prion disease and are therefore likely to be independent of prion strain.
Approximately 1,000 HS mice at generation 37 were inoculated intracerebrally with Chandler/RML prions. Incubation times (in days) were determined (n=1,052) and were used as the quantitative trait. The incubation times conformed to a normal distribution (Anderson–Darling normality test) with a mean of 147±15 (SD) with a range of 103–229 days, thus confirming that both "long" and "short" incubation time alleles segregate in the population. To reduce the amount of genotyping, we analysed the mice from the extreme 20% of both ends of the incubation time distribution (approximately n=400) as this contains most of the power available in the cross. Twenty microsatellite markers from Mmu15 (D15Mit234–D15Mit43) at approximately 1–3 cM intermarker distances were genotyped in the HS mice (Electronic supplementary material, Table 1). Multipoint linkage analysis was carried out using the HAPPY programme (http://www.well.ox.ac.uk/happy) [17] which identified a peak of linkage at the interval D15Mit241–D15Mit262 (−logP=4.52) and the adjacent interval D15Mit262–D15Mit34 (−logP=4.48; Fig. 1a and Electronic supplementary material, Table 1). Significant linkage was taken as −logP>3 as defined by a permutation test (n=1,000) carried out by HAPPY. This QTL explains 7.3% of the observed variance which is similar to that reported for BSE prions. Based on information from the UCSC Genome Browser NCBI build 37 (http://genome.ucsc.edu), the region from D15Mit241–D15Mit34 is 1.2 cM (3.6 Mb), which represents a considerable reduction from the original locus and is small enough for a candidate gene approach to be feasible.
Current database interrogation (NCBI build 37) estimates that the D15Mit241–D15Mit34 interval contains 39 known transcripts. D15Mit241–D15Mit262 contains only one Genbank transcript (AK020896), which contains three exons with the potential to encode a protein of 70 residues and is of unknown function. D15Mit241 maps within the intron 2 of this transcript. Sequencing AK020896 in the HS parental strains identified two polymorphisms in exons 1 and 2, respectively. These are likely to represent SNPs in the 5′UTR and map proximal to D15Mit241. We did not exclude AK020896 on this basis; however, we extended our analysis to include the D15Mit262–D15Mit34 interval (3.47 Mb). This contains 38 transcripts of which we sequenced 29 in the HS parental strains (see Electronic supplementary material, Table 2 for a list of sequenced genes). These 29 genes include all genes in the region predicted to have a role in the central nervous system. Excluded genes included those with known non-CNS tissue-specific functions. Sequencing was not exhaustive, and we focused primarily on the cDNA transcript (ORF, 5′ and 3′UTRs), intron–exon boundaries and potential promoters as defined by the literature for each gene or PROSCAN (http://www-bimas.cit.nih.gov/molbio/proscan). Most genes proved not to be very polymorphic in the HS parental strains, with only 88 polymorphisms being identified across the whole region. Most genes contained one or two polymorphisms, but the greatest number were seen primarily in Rabl2a (33) and Cpne8 (38). All variants were assessed using a function of HAPPY that uses progenitor strain information to identify quantitative trait nucleotides by reconstructing ancestral haplotypes [22]. The most significant candidates from this analysis were: Rabl2a (−logP=6.02), Syt10 (−logP=5.4) and Cpne8 (−logP=5.1). A graphic display from HAPPY is shown in Fig. 1b, and details of all identified individual SNPs are shown in Electronic supplementary material, Table 3. For Rabl2a, the most significant strain distribution pattern (Table 1) is seen for two SNPs, one of which is a non-synonymous amino acid change (S276G). For Syt10, only one SNP reaches the level of significance, and this is a synonymous change in exon 1 (G46G). For Cpne8, nine significant SNPs were detected, six of which were intronic and three were seen in the 3′UTR.
To verify these data, we genotyped representative significant SNPs from Rabl2a, Syt10 and Cpne8 in the HS animals and analysed the data by ANOVA (Table 2 and Electronic supplementary material, Table 5). Surprisingly, the Rabl2a SNP was not significant (P=0.84), but SNPs for Syt10 (P=0.0019) and Cpne8 (P=0.0002) were both highly significant. The original linkage data for Mmu15 came from a C57BLxRIII cross which suggest that if we are detecting an effect from the same gene in both crosses, then the functional polymorphism should be present in both crosses. We therefore sequenced the candidate SNPs in RIIIS/J and compared this to the C57BL/6J alleles. For the single Syt10 SNP (Gly46Gly), C57BL/6J and RIIIS/J share the same allele, which suggests that this is unlikely to be the QTN. For the nine significant Cpne8 variants (six intronic, three in the 3′UTR), C57BL/6 and RIIIS have different alleles, which is consistent with Cpne8 being the most promising candidate gene in this region. No clear function can be assigned to the Cpne8 SNPs; however, it is possible that because our sequencing was not exhaustive, we have not seen the functional SNPs but that they share the same strain distribution pattern. Using RT-PCR, we examined the Cpne8 transcript across different mouse strains for splice variants, but at the level of detection by ethidium bromide-stained agarose gel, we could not detect any alternative splicing events (data not shown).
As an additional screening tool, we also looked at the expression of mRNA by real-time RT-PCR in the HS parental lines for Cpne8 (Fig. 2a). RNA was extracted from whole brains of 6- to 8-week-old male mice (except LP). Samples were analysed by real-time RT-PCR and normalised using the geometric mean of the quantity obtained from three endogenous controls (GAPDH, β-actin and Thy-1). To look for genotype-related differential expression, the strains were grouped according to the most significant strain distribution pattern seen in Cpne8 as represented by the 3′UTR SNP (CPNE3UB T/C) where T = A, C3H, CBA and C = AKR, BALB, C57, DBA. No significant difference in expression was seen between the two genotypes (Fig. 2b). We also compared the level of Cpne8 expression between C57BL/6 (CPNE3UB C) and RIIIS (CPNE3UB T), and no significant difference was detected (Fig. 2c). In addition, we compared Cpne8 mRNA expression with published incubation times for the inbred lines, and no correlation was observed (R2=0.28) [3, 4, 23].
To see if Cpne8 mRNA expression is associated with prion infection, we compared Cpne8 levels in the brains of uninfected and RML prion infected mice at the terminal stage of disease (Fig. 2c). A significant increase (×2.5-fold) was seen in disease in both C57BL/6 (P=2.7×10−5) and RIIIS strains (P=7.8×10−6). No significant difference was seen between RML-infected C57BL/6 and RIIIS strains.
Whilst multiple QTL for prion disease incubation time in mice have been described, with the exception of Hectd2, no candidate genes for these loci have been identified [7–12]. Using a heterogenous stock of mice, we have successfully reduced the size of the Mmu15 QTL from approximately 25 to 1.2 cM. Sequence analysis and subsequent testing of the SNPs identified many genes, with SNPs reaching the threshold of significance (−logP>3); however, Syt10 and Cpne8 were identified as the most promising candidate genes. In studies of this nature, it is not always possible to eliminate the possibility that genes excluded by us may still be contributing to the QTL. Further, our sequencing of the region was not exhaustive, and it is possible that the most significant strain distribution pattern could be seen in other genes or intergenic regions.
Although SNPs in both Syt10 and Cpne8 gave highly significant results, we discounted Syt10 because these were not polymorphic between C57BL/6 and RIIIS strains. This was based on the assumption that the quantitative trait gene and nucleotides are the same in the original C57BL × RIII backcross and the HS. Although a reasonable assumption, it is possible that this may not be the case because we used C57BL/6J mice, and the original cross used C57BL mice which may harbour differences between them. In addition, the original QTL was identified in mice inoculated with BSE prions, whereas we have used mouse-adapted scrapie prions which are known to be distinct prion strains.
Based on the assumptions detailed above, Cpne8 is the most promising candidate gene in the region. Highly significant SNPs were found in the 3′UTR. These SNPs were not associated with an alteration in mRNA level; however, it is possible that they influence mRNA structure and translation efficiency. Although we sequenced the putative promoter, 5′UTR, ORF and 3′UTR of Cpne8, it is possible that we may not have seen the functional SNPs if these are contained within introns or more distant regulatory regions.
Differences in mRNA expression levels were not associated with genotype or incubation time differences; however, we did see a ×2.5-fold increase in the disease state independent of mouse strain, suggesting that Cpne8 may be involved in the disease process.
Little is known about the function of Cpne8; however, it is a member of the copine family of proteins which are Ca2+-dependent phospholipid binding proteins thought to be involved in membrane trafficking [24]. PrP is a glycosylphosphatidylinositol anchored protein located primarily on the plasma membrane. During its synthesis, it is trafficked through the endoplasmic reticulum and Golgi towards the plasma membrane where it is associated with lipid rafts. PrP is thought to be constantly recycled from the plasma membrane through clathrin-mediated endocytosis (for review, see [25]). The cellular location of PrP conversion to the abnormal form, PrPSc, and the importance of these sites in prion pathogenesis are yet to be fully described. However, pathogenic mutations of PrP, associated with familial prion disease, have shown abnormal cellular localisations [26–28]. This suggests that the correct trafficking of PrP is critical and proteins such as CPNE8 may be important in regulating this process. Although Syt10 is not a good candidate gene for the C57BL × RIIIS cross, it is highly significant in the HS, and interestingly, Syt10 is a member of the synaptotagmin family which are also thought to be Ca2+-dependent phospholipid binding proteins [29].
In conclusion, we believe that Cpne8 is the most promising candidate gene for the Mmu15 QTL. This is the first example of a prion disease incubation time QTL being reduced in size by fine mapping and for a candidate gene to be identified. Similar approaches applied to other QTL across the genome should reveal other candidate genes that will elucidate the key pathways involved in prion disease pathogenesis.
Below is the link to the electronic supplementary material.
Linkage analysis for microsatellite markers Mmu15 (DOC 39 kb)
Sequenced RefSeq genes from interval D15Mit262–D15Mit34 (DOC 40 kb)
Analysis of polymorphisms from D15Mit37–D15Mit34 (DOC 138 kb)
Primer and probe sequences for SNP genotyping by allelic discrimination (DOC 32 kb)
ANOVA analysis of polymorphisms genotyped in HS mice (DOC 34 kb)
We are grateful to Robert Hitzemann (Department of Behavioural Neuroscience, Oregon Health Sciences University, Portland, Oregon, USA) for the kind gift of the Northport Heterogeneous Stock of mice, David Key and his staff for animal care and Ray Young for preparation of figures. This work was funded by the Medical Research Council, UK.
Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Another playoff game in Toronto, another odd t-shirt design.
NHL 1hr ago
Capitals' Justin Williams celebrates series clinching win by getting a speeding ticket
Maybe Williams should change his nickname from Mr. Game 7 to Mr. Lead Foot.
NFL 2hr ago
Heartwarming NFL draft hype video relives the event's most emotional moments
I'm not crying; you're crying.


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BCSD School Budget Vote is Tuesday, May 16, 2017 in the BCHS Upper Gym from 7 a.m. to 9 p.m.
Filed in Archive, BCHS, District by joellen.gardner on April 27, 2017
Students honored in April by Board of Ed
Several students were honored recently for their scholastic achievements, athletic accomplishments and fine representation of the Bethlehem Central School District.
Filed in BCMS by joellen.gardner on April 27, 2017
Congratulations, National History Bowl teams
Competing against high school students from all over the U.S., our BCMS History Bowl teams finish first and second in their divisions.
Filed in Archive, Budget News, District, District News by joellen.gardner on April 26, 2017
Meet the BOE Candidates on May 2
On May 16, voters will elect three members of the Board of Education. Eight candidates will appear on the ballot.
Filed in BCHS by hanna.teal on April 26, 2017
BC senior receives Youth Award from Chamber of Commerce
BCHS senior Andrew Taylor will accept the Youth Award at the 32nd Annual Chamber Awards Dinner.
Filed in BCMS, District by joellen.gardner on April 10, 2017
BCMS Odyssey team advances to World Finals
A team of middle school students earned the right to compete at Michigan State in May.
Recap of March 8 Community Conversation
Video and frequently asked questions about the future of Clarksville Elementary School.
Filed in District by joellen.gardner on March 23, 2017


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My pee smelled like sake this morning it was sooo disgusting.
How is Miami?
Omk. I'm shitggaved om loincoln
Let's make jello shots for tomorrow
What's going on tomorrow?
Nothing, it's Wednesday
For those pictures, I will suffer this headache.
25 People Reveal The Creepiest Kids They Went to School With
i threw up on the table at the pizza place and peed in her room mates closet. i wouldnt invite me back either
I'm pretty sure my moms getting nailed in the bathroom right now while I'm chaperoning. God damn it.
I sent him pictures of just me in my thong and he replied "you're so sweet, you make me feel special <3".... Oh.
There are reggae songs being written about me...where have I gone wrong in life?
Just do let me go home with anyone especially I a guy with a hair sweater
17 Subtle Body Language Signs That Reveal A Lot About Someone
Why am I the only one golf clapping for the vomiting girl on the train who just fell of her seat into her own vomit
I mean I love some drunk compliments, but he just wasn't up to my low standards.
Btw I don't have words to express my appreciation at how many times you've had to be on a dirty bar bathroom floor for me in the past two weeks
at one point while they were drilling into my jaw I just remember thinking "will I ever be able to suck dick again"
I think all three of us just need to suck it up and go to lunch with him to keep our bar tab down
My boobs are hoarders, they steal food and hide it. Greedy bitches.


text:
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The Samson Nano - Further Innovations in Pallet Wrapping
Posted: 24 January 2017
Since its launch in 2013, Samuel Grant Packaging's Samson Nano has revolutionised the way in which pallet wrapping is both undertaken and charged for.Read Full Post
Find out our latest company news and events.
Posted: 24 January 2017
Since its launch in 2013, Samuel Grant Packaging's Samson Nano has revolutionised the way in which pallet wrapping is both undertaken and charged for.Read Full Post
Posted: 01 November 2016
Leeds-based Samuel Grant Packaging is delighted to announce their new collaboration with the United Bank of Carbon - a registered charity run from the University of Leeds that helps forge partnerships between businesses and international conservation initiatives.Read Full Post
Posted: 23 September 2016
Through our Transport Manager Tim Howcroft, we are delighted to be sponsoring Kippax Templars Badminton Club. Kippax Methodists badminton club was formed in 1953 and was one of the founding members of the Castleford & District Badminton League. Since a 2004 merger with another local successful club, Templars Badminton Club, Kippax Templars Badminton club has gone from strength to strength.Read Full Post
Posted: 04 February 2016
Here at Samuel Grant Packaging we love supporting local teams. Holbeck Bowling are just one, and we have proudly sponsored them for the last three years. During this time we have bought them team shirts and paid their team fees.Read Full Post
Posted: 19 January 2016
It's been a very special week this week here at Grant's as we have celebrated our 125th AnniversaryRead Full Post
Posted: 09 October 2015
Over 100 staff, contributors and local supporters turned out on Thursday 8th October to celebrate the launch of Samuel Grant Packaging's new warehouse on the Leeds City Enterprise Zone.Read Full Post
Posted: 21 September 2015
Samuel Grant Packaging are delighted to announce their relocation. The company's brand new, purpose built facility is on the Thornes Farm Industrial Estate, part of the Leeds City Region Enterprise Zone. It boasts 50,000 square feet of warehousing space and will allow Samuel Grant Packaging to continue to expand their offering of Value, Quality & Service for which they have become well known throughout history.Read Full Post
Posted: 03 August 2015
You may have noticed a change! Following numerous discussions, and prior to our big move at the end of August, we have decided to change the trading name of the company to Samuel Grant Packaging.Read Full Post
Posted: 03 July 2015
Congratulations to Carla Dobson, the North East's Samson Nano Systems Specialist, who has scooped the UK Warehousing Association award for Junior Manager of the Year!Read Full Post
Posted: 22 June 2015
Taylors of Harrogate have reached the anniversary of having the Samson Nano pallet wrapping solution in their Harrogate distribution centre, and in turn are celebrating the saving of almost 10 tonnes of C02 emissions.Read Full Post
Posted: 18 May 2015
Samuel Grant Group are delighted to announce that they have scooped the prestigious Gold Award for Warehouse Innovation at the 2015 Intralogistics Awards.Read Full Post
Posted: 28 January 2015
Construction work on a new £5 million facility for Leeds packaging company Samuel Grant Group Ltd has started on site in the Leeds City Region Enterprise Zone, bringing the amount of new employment space currently under construction and committed to 425,000 sq ft, with the potential to create hundreds of new jobs.Read Full Post
Posted: 05 January 2015Read Full Post
Posted: 03 December 2014
Marmax Products won the Green Champion Award at the Chronicle Champions North East's Inspirational Heroes Awards ceremonyRead Full Post
Posted: 28 November 2014
Marmax Products won the Green Champion Award at last night's Chronicle Champions 2014 Award ceremonry which was held at the Biscuit Factory in Newcastle Upon Tyne. Everyone at Marmax Product is really happy at winning this award and proud at what the company has achieved.Read Full Post
Posted: 08 October 2014
County Durham company Marmax Products nominated for top green award after transforming seven million empty milk cartons into furnitureRead Full Post
Posted: 25 September 2014
The North East manufacturers that will battle it out at Insider's Made in the North East Awards 2014 have been announced.Read Full Post
Posted: 31 July 2014
Following major success and industry recognition, Marmax Products was asked to feature on BBC Radio Tees yesterday.Read Full Post
Posted: 31 July 2014
Following major success and industry recognition, Marmax Products was asked to feature on BBC Radio Newcastle's morning show recently.Read Full Post
Posted: 09 July 2014
Marmax Products has been announced as a finalist in the Best Recycled Plastic Product of the Year category for this year's Plastics Industry Awards (PIAs). The 2014 glittering black-tie event takes place on Friday, 24 October at the London Hilton on Park Lane and will be hosted by comedian and TV presenter Al Murray, aka The Pub Landlord.Read Full Post
Posted: 04 March 2014
Marmax Products Ltd is delighted to be sponsoring a Northumberland initiative to encourage schools to reduce, reuse and recycle.Read Full Post
Posted: 26 November 2013
Marmax Products Ltd, based in Stanley, County Durham, have been presented with a Green World Ambassador insignia at a prestigious presentation ceremony in The House of Commons.Read Full Post
Posted: 23 October 2013
Ouir Sheffield branch will be raising money this coming friday the 25th October in aid of Breast Cancer Awareness.Read Full Post
Posted: 14 October 2013
15" x 18" x 3" plain white patch handle carrier bags now available from stock.Read Full Post
Posted: 02 October 2013
A huge thank you to all who donated to our World's Biggest Coffee Morning event in aid of Macmillan Nurses.Read Full Post
Posted: 27 September 2013
Our Sheffield branch is taking part on the "World's Biggest Coffee Morning" in aid of MacMillan nurses.Read Full Post
Posted: 05 August 2013
Available in five different print styles from stock.Read Full Post
Posted: 13 May 2013
The Next Generation In Stretch Film.Read Full Post
Posted: 08 April 2013
We are pleased to announce the introduction of our new Special Offers page to the Samuel Grant website.Read Full Post
Posted: 21 March 2013
We are pleased to announce that the very first issue of our Group newsletter is available to download now.Read Full Post
Posted: 20 March 2013
We are delighted to announce the addition of the FoamPlus Packaging system to our product portfolio.Read Full Post
Posted: 21 February 2013
The Samuel Grant Group YouTube channel is now up and running, at the following link:Read Full Post
Posted: 19 February 2013
Following the sucessful re-launch of our website, we have the first major update coming soon.Read Full Post
Posted: 05 February 2013
The Leeds office have managed to raise a staggering £2492.40 in aid of St Gemma's Hospice in Leeds (http://www.st-gemma.co.uk).Read Full Post
Posted: 04 February 2013
Thank you for visiting the brand new, re-launched website for the Samuel Grant Group.Read Full Post
© Copyright Samuel Grant Group Ltd 2017. See our Terms & Conditions | Privacy Policy | Our Policies
Created by Edward Robertson web design with the Responsive Grid System.


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Adding or removing apps pinned to the Unity launcher in Ubuntu isn't a big deal - just do a right click and select the relevant option to remove an app icon from there, and drag and drop an icon on
How to make Ubuntu's Unity shell lightweight
Love Ubuntu's Unity shell, but hate the performance overhead it brings especially when dealing with systems having low resources? Worry no more, as in this tutorial, we will discuss how you can make Unity lightweight. But before we do that,
How to display dialog box containing Lock, Suspend, Restart, and Shutdown icons from command line in Ubuntu
If you are an Ubuntu user, you might have observed the dialog box that gets displayed when you quickly press and leave the power button. The dialog box contains icons to Lock, Suspend, Restart, and Shutdown the system. Have you
How to remove margins and bring down PDF file pages to their minimal size
If your work involves reading a lot of PDF files, I am sure you'd have sometimes thought about removing those empty margins from PDF file pages in order to get a better reading experience on your hand-held device, like your
How to get Uber time-to-pickup and pricing information through Linux command line
Are you a Linux command line user? Are you an Uber fan as well? If your answer to both the questions is yes, then you'll be glad to know that you can easily get Uber time-to-pickup as well as pricing
How to access Ubuntu 16.04 gnome-calendar app from system tray
In case you don't already know, the version 16.04 of Ubuntu LTS OS brought along a couple of new apps, with GNOME Calendar being one of them. Start Gnome Calendar You can launch the app from the command line: gnome-calendar
How to enable/disable Alt+Tab type switching for windows of same application in Ubuntu
If you are one of those who prefer using keyboard over mouse wherever and whenever possible, I am sure you must be aware of what Alt+Tab does. For starters, this key combination lets you switch between windows of the currently
How to change wallpaper in Ubuntu based on local weather or time
While there are users that just don't care about wallpapers, there's also a bunch which is very specific when it comes to their desktop backgrounds. If you fall in the latter category, we have something very interesting for you: a
How to stop mouse scroll from switching applications in Ubuntu Unity
Consider a situation: You accidentally move your mouse pointer to over the Ubuntu launcher while browsing a website in a web browser. And then when you try scrolling the webpage using mouse, you instead end up switching to the app
How to Configure WDS on Windows Server 2012 R2 with DHCP Server Running on Linux
This tutorial describes how to deploy WDS when a Linux DHCP server is running on your network. I am using Linux Ubuntu a 14.04 server distribution for the DHCP configuration. Prerequisites Make sure: The administrator account has a strong password. A


text:
Dear Jay-Z,
Oh, Jigga-Man, how do you do it? You are a rap icon, business mogul, cultural talisman. So many things to so many of the populace. How do you keep up with such a scorching little number like Ms. Knowles? Must be tiring as Hades, waiting for a team of stylists and couturiers to dress and wash this princess of moving pictures set to music! And when I saw this clip online, I was entranced. Never before has a peek-a-boo of a flesh-toned undergarment caused such a clamor among all the limpwristed and haughty constabulary.
I applaud you, sir. A less rational and civilized gentleman would sooner married Trina than toil in obscurity behind an international treasure – with the possible exception of Malaysia – like B. I tip my cap to you, Hova. The gods on Mount Olympus are surely smiling on you.
Craig Hlavaty
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In Case You Missed It
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TicketsTue., May. 30, 8:00pm
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The initial spirit and intent of the program was to enhance the capabilities and effectives of anti-terrorist campaigns in Mauritania, Mali, Chad, Niger, Nigeria, Senegal and Burkina Faso. The TSCTP has been successful in slowly building capacity and cooperation even in the face of adversity; however there is much work that is yet to be done.
Read the full story at milsatmagazine.com.
4/25/2017Gogo Bolsters Inflight Connectivity Capacity over North America and the Pacific with SES
4/25/2017US Pay TV Providers Launch First Linear Consumer Ultra HD Services with SES
4/25/2017ETL Systems RF equipment selected by major U.S. broadcast network
5/11/2017WTA Webinar: Understanding Teleport Certification
5/15/2017 » 5/16/2017MilSatCom Asia-Pacific
5/17/2017 » 5/18/2017East Africa Com 2017
5/22/2017CASBAA Satellite Industry Forum 2017
5/23/2017 » 5/25/2017WTA Executive Dialogue Series @ CommunicAsia 2017


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Explore Party Travel, Travel Food, and more!
Explore related topics
Planning a road trip to Chicago? Here's how you can do it: Plan a fun Road Trip to Michigan to Chicago
Heading to Wisconsin on a road trip? Grab a selfie—and maybe a tour—at these fun roadside attractions: http://www.midwestliving.com/blog/travel/7-wisconsin-roadside-wonders/
10 Activities To Do In Scenic Bear Lake Valley
Best activity in Vegas with the Girls - Afternoon Tea at the Mandarin Oriental Las Vegas // localadventurer.com
The experience of the open road can be one of the most invigoratingly free feelings in the world - make sure you do it right by being prepared with the right tools. One of the most important of which is to remain hydrated - but not just any beverage will do. You have to reach for something that's familiar, yet new and adventurous at the same time - much like the open road. You have to reach for something extraordinary. Check out perrier.com and find it.
Interstate 95 is filled with unique attractions and stops that you don't want to miss.
Have you planned your summer vacation? Don't miss my bonus packing tip in addition to the top 10! It's the best one of all!
M22 - #Michigan
Boca Raton Resort & Club, A Waldorf Astoria Resort. Valentine's Day weekend - or ANYTIME getaway!
Maine Scenic Drives: Schoodic Scenic Byway - HowStuffWorks


text:
- Sign in
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Coastal Beach House with screened pool
From $100 / night
Payment Protection
balance of due
Description from the manager
Description from the manager
Bedrooms: 4/
Bathrooms: 2/
Sleeps: 8/
Type: House
Book this 4 bedroom, 2 bathroom house in Naples today! A total of 8 people can sleep here comfortably. This property has been booked 2 times.
Guests can enjoy a air conditioning, internet access, and DVD among other amenities offered by this rental.
Travelers are sharing their opinions on this property. It has an overall rating of 5/5 thanks to 1 review.
The owner responds to 70% of inquiries, which is lower than average.
About the manager
About the manager
Kaelyn R.
Kaelyn R.
- Response rate: 70%
- Response time: Within a day
Overall rating:
4.7 / 5.0
Proximity to TripAdvisor's Top Naples Attractions
Proximity to TripAdvisor's Top Naples Attractions
Reviews (0)
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News from State Street
Regional news from newspapers and blogs
State Street
- Will a new agency improve Vermont's digital services?WCAX
- MGold's tempered climb makes gains more 'sustainable', says Milling-Stanleymarketwatch.comGermany , France , Russia , Syria , North Korea
- StoriesEtc: 'Deflated' captures tolls of silent sadness, untreated traumabadgerherald.com
- Are You Guilty of Using Purpose as a Marketing Tactic? Customers Can See Right Through YouInc.
- Can Savannah create good parking karma?connectsavannah.comChippawa , Chatham , State Street , San Francisco , Savannah
- Regional farmers markets worth a visitmcall.com
- Patton Financial Advising: Economic Outlookexploreclarion.comNew York , Riau Islands , Armour , State Street , Alaska
- UW fails to foster environment that celebrates, encourages learning itselfbadgerherald.com
- Holocaust speaker's 'voices' recall horror, sadness of pastThe Daily Gazette
- Santa Barbara Marches for Scienceindependent.com
- Raffle to help State Street Saloon fire victimsSeacoastonline.comFerry Landing , Rye , Redhead , Cava , Brie
- Eyewitness video of apartment fire on Cambridge Drivekentucky.comIraq , Kentucky , State Street , Winchester , Sèmè
- MI-291 Exit 1A in Springfield to close tonightmasslive.com
- MFrench bond spread narrows to 4-month low as 'Frexit' fears abatemarketwatch.com
- Portsmouth police log: April 21-22Seacoastonline.comGrant Avenue , Maine , Maplewood , Dover , Grafton
- The Santa Barbara Questionnaire: Joey Benaronindependent.com
- Portsmouth Police Log: April 21 to April 22Seacoastonline.comPortsmouth , Grant Avenue , Maine , Maplewood , Dover
- Beam me up, Scotty: Boise store...KTVB
- Hundreds walk in New London to end homelessnessThe Day
- The latest on how Mill Artisan District is shaping upThe Daily GazetteNew York , The Gateway , North Church , Erie , The Mill
Frequent names
Regional sources
- Boston Herald
- Ppatriotledger.com
- eagletribune.com
- Ttelegram.com
- eastbayri.com
A newspaper or a blog is missing here? Suggest a source


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Are you a NSW Member? Have you employed someone in a new position since 1 July this year and don't have a payroll tax liability? You may be eligible for a $2000 grant from the NSW government – go this link to find out more here.
NSW Grant OpportunitiesNews Editor