text: CHICAGO, Feb. 22, 2013 /PRNewswire-USNewswire/ -- The North Park University Gospel Choir Touring Ensemble will visit seven Evangelical Covenant Church (ECC) congregations in California during its 2013 spring tour, singing in worship and in longer choral performances. The tour theme is "Lifting the Highest Praise." The March 10-17 tour includes visits to congregations in Fremont, Modesto, Turlock, Hilmar, Santa Rosa and Oakland. Of the approximately 130 students in the popular choir, 51 students make up the touring ensemble. The choir is directed by Stephen Kelly, worship coordinator, University Ministries, and co-directed by Dr. Helen Hudgens, associate professor, School of Music. Instrumentalists will accompany the touring ensemble. Performances dates and locations are at http://www.northpark.edu/News/Featured-Events/2013-Gospel-Choir-Touring-Ensemble on the Web. Students have committed their entire spring break to be part of the California tour. "It inspires me over and over again to see God at work in the church, and to see these students become the body of Christ, and sing so passionately about the goodness of God," Kelly said. About 10 students on the tour are from Scandinavia, South America and Asia, he said. The choir tour will feature diverse compositions by well-known and award-winning composers. Among the songs the Gospel Choir Touring Ensemble will sing is "High and Lifted Up," by Joe Pace; "Faithful," Hold Out," and "Grateful" by Hezekiah Walker; "Jesus be a Fence," written by by Sam Cooke, the legendary American pop and soul singer, and arranged by Fred Hammond; "Total Praise" by Richard Smallwood, a gospel music icon; "More than Anything" by Lamar Campbell; "I Will Run" by Freddy Rodriguez, and others. Two favorites are "Awesome God Medley," and " Brighter Day" written by Kirk Franklin, a seven-time Grammy Award winner. Gospel music, rooted in the black church experience, is joyful, filled with praise and uplifting. Choir members are frequently in "step motion" while singing, and clap their hands. There's also a story that goes with every choral piece, Hudgens said.


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Subject: | What would the latinate forms of my names be? |
Author: | Anthony (Authenticated as Anthony) |
Date: | August 3, 2004 at 9:42:10 PM |
Anthony DavidI assume Anthony would be Antonius, but what would david be?
Because this message is archived you cannot respond to it. |
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subset: commoncrawl


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But he didn't deny the spasms...interesting.
But he didn't deny the spasms...interesting.
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McGrady at 20 was still a raptor not even on the magic. That other "shoe company" is obviously adidas. Mcgrady was not with adidas either at age 20.
I heard tmac and kobe played 1 on 1 in all-star weekend once and TMAC owned him.
Kobe is just full of ****.
So some guy on PSD says he saw them in Germany lol? It's probably a Kobe Rider making up nonsense.
I love Kobe but he is taking his mouth to another level only if he still had game.
He got owned by Wade and Bron last night.
People are so naive and forgetfull. Tmac could have easily done more if he was paired up with SHAQ. Like it's not that hard to realize that. He had just as much going for him on the court as Kobe. Kobe, when faced with a supporting cast like Tmac was in Orlando put up good numbers but didn't get past the first round? the ****?
I know winning is the most important aspect with professional sports, but really they push it so much in the NBA when they know it's out of a player's reach most of the time. Really the GMs should get hyped on sportscenter when it comes to winning more...not ONE Superstar, who was given the gift of an extremely good cast..
same goes for Iverson, Carter. They wouldn't get any nonsense criticism about whatever. I hate being in the minority on this too. Iverson chucked a bad percentage BECAUSE HE HAD TO score a lot to win. Carter never won a ring BECAUSE you cant name his second best player on the Raptors, you cant name his bigmen on the Nets.
Seriously what have Kobe, even Jordan won, without a good enough supporting cast? Pippen was a superstar on any other team, Kobe always had a stacked cast when he won.
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Tmac lost his balls vs Kobe around 06 or 07 IIRC. I dont just mean as a result of his decline either, I mean he appeared to succumb to whatever Kobe wanted (in terms of positioning) when before, regardless of his cast/injuries, he went at him, good or bad it was on his terms. Didn't want to admit it then but that should have been the sign that the fight in him to rise above the moment was over.
I didn't leave my bed, because I was like, 'Man, if I walk outside somebody might just try to hit me with their car or say anything negative to me
-Beta B KD
very true. It sucks, the Rox window was so short because of it I think. McGrady, even before the shoulder and back injuries, appeared to check out for periods of time.
Also, need to remove the Toronto days in that matchup comparison statistically.
I just find it unfortunate that the Orlando, even early Houston McGrady ended up like he did. He was so awesome for a handful of seasons.
If you want the ultimate, you've got to be willing to pay the ultimate price. It's not tragic to die doing what you love.
Why? TMac is irrelevant while Kobe is still an all star. I feel like if anything this looks bad on Kobe, and really doesn't do anything to TMac's image.
I feel like since he said "I've never been to Germany with Kobe". That's pretty much all she wrote. I mean if until there's proof that Kobe, and TMac were in Germany together it's basically false **** by Kobe IMO.
Tell them truth TMAC!
[Packers/Lakers Lover]
"If Rodgers were even just simply as good as Brady or Manning in the playoffs, his team would almost never win. Instead, what we've seen is a super hero act."
-Peter Bukowski
At least you are a reasonable person unlike most of the people in this thread. I believe Kobe just because he has nothing to gain by lying. It's not ego, his career crushes McGrady's so it's not like he has something to prove against him. McGrady probably has a pretty bruised or broken ego about now so I could see him denying it.
I will say prime McGrady was just as good as Kobe at that time. Their numbers were pretty similar from what I remember. My best friend in middle school was a McGrady fan and I was a Kobe fan and I remember for at least like 2 years were there wasn't a clear better player, unless you want to take winning into consideration.
Saluti, Vino!
Saluti, Vino!
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Is this your DreamBerry? A video rendering of the BlackBerry 10 London
0. phoneArena 21 Sep 2012, 15:44 posted on
With only a few pictures to work off of, a rather talented designer put together a very nice looking video rendering of the BlackBerry 10 touchscreen "L-series"...
This is a discussion for a news. To read the whole news, click here


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Oorn Noth: Racer on the Run
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Oorn Noth: Racer on the Run is the first installment in the Wizards of the Coast column "Bounty Postings". Written by Eric Cagle and published on May 14, 2008, it provided a bio and stats for the Zabrak racer Oorn Noth.
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2AM's Jinwoon recreated the famous scene from the Beatles' album 'Abbey Road'.
Jinwoon stopped by the famous landmark and did a good job in recreating the famous cover. Jinwoon traveled to London back on January 3rd, and during his trip he visited numerous famous landmarks such as the Big Ben, the Dominion Theatre, and attended a Queens Park Rangers F.C. match to watch Park Ji Sung play.
It seems Jinwoon is having a great time in London! Fellow 2AM member Jo Kwon is also in Europe, traveling all over the continent including visits to Switzerland, England, France, and more.
[gallery columns="4"]
Source: Jinwoon's Twitter
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Henley & Partners Global Citizen Award
Once a year, Henley & Partners honors an inspirational global citizen who has made an extraordinary contribution towards improving the global community.
The Global Citizen Award consists of a specially made commemorative medal, an Award Certificate signed by the President of the Award Committee and USD 50,000 monetary prize, of which USD 25,000 is donated to UNHCR to support the global refugee cause.
The annual laureate of the Global Citizen Award is chosen by the Award Committee, an independent body of distinguished individuals, through a majority decision. The Award Committee is comprised of the following members:
- Her Royal Highness Princess Firyal of Jordan
- Her Excellency Marie Louise Coleiro Preca, President of the Republic of Malta
- Senator Joëlle Garriaud-Maylam, Secretary of the Standing Committee on Foreign Affairs, Defense and Armed Forces, Senate of France
- Professor Dr. Khalid Koser OBE, Executive Director of GCERF and Vice-Chairman of the Advisory Council on Migration, World Economic Forum, Geneva
- Fr. Dr. Marek Urban CSsR, Councillor of the Henley & Partners Foundation, Krakow
- Namira Salim, Artist, Polar Explorer and Virgin Galactic Founder Astronaut, Monaco
- Leigh Foster, Chief of Events, Campaigns and Goodwill Ambassadors, UNHCR, Geneva
To read more about the 2015 recipient of the Global Citizen Award, please click here.
To read more about the 2016 recipient of the Global Citizen Award, please click here.


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I know a lot of people are wondering if former UCLA center Alfred Aboya will get a chance to play in the NBA, and his former teammate, Darren Collison thinks it could happen.
"I think he's going to impress a lot of teams," Collison said. "He has some versatility and he can make the 15 foot jump shot. And with how hard he plays –that's what the NBA is looking for, how hard you play — I think he can play."


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Enjoy a weekend getaway to a town with quite the knack for preserving the past.
The southern New Hampshire town is a window to the past.
June is the best time to catch the meadows of Sugar Hill lit up with blooming lupine. Here's your guide to a weekend visit.
The Star Island Corporation is celebrating its 100th year of "creating an environment that frees all who come to renew spiritually, explore matters of consequence, and gain knowledge about the world as it might ideally be." Perhaps it's time you paid a visit to our special island.
We take advantage of beautiful September weather to explore historic villages close to home.
A trio of towns — Pittsburg, Clarksville and Stewartstown — fill the narrow triangle at the tip of the state, a vast area of forests, lakes and streams and headwaters of the Connecticut River.
Summer is the perfect time to visit Tamworth as it hosts a historic summer theatre venue, the Remick Country Doctor Museum and Farm, a great farmers' market, picturesque hiking and more.
A food-focused road trip from Jackson to Gorham including gorgeous scenery, several shops, things to do and, of course, great places to eat.
We begin at one of NH's prettiest lakes and explore the two towns that guard it — Ashland and Holderness.
Those lazy days of late summer are perfect for a weekend of exploring New Hampshire's secret lake


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Mighty Peep Jousters warming up for Battle!!!!! To become a Master Peep Jouster one must become one with the Peep, so say the great masters!!!!!
The terrible, awful and sad sight of melted peeps after battle most savage ): It appears the victor in this terrible battle is the peep with the reddish toothpick.
Sir William the Terrible eating one of the poor melted peeps after it has been wounded beyond all hope of healing (it really was the most humane thing to do).
WARNING: To all out there thinking of eating melted peeps, please make sure they have had time to properly cool before doing so. Otherwise a burnt mouth could ensue and that is a big Ouchy!!!!!!!!!!!!
To see some peep jousting in all its real life glory, take a look at this YouTube video: http://www.youtube.com/watch?v=JBBzTvlTuGw.
Pretty incredible wouldn't you say? This sport has changed my life!!!!!!!!!
And now for something completely different, book reviews by Sir William the Terrible:
Sir Gawain and the Green Knight by Selina Hastings and Juan Wijngaard - This is not exactly a new book but it is an excellent adaption of the "Sir Gawain and the Green Knight" tale from Arthurian Legend. This story is about a young Sir Gawain of King Arthur's Knights of the Round Table and how his courage and sense of chivalry is tested by the eerie Green Knight. Here is an excerpt from the story to wet your taste buds:
'A noble opponent indeed!' exclaimed the Green Knight, dismounting from his horse. 'Sir Gawain, you must swear that in exactly one year and a day you will seek me out and allow me to return your blow.'
'I give you my word,' said Gawain.
Then the Green Knight knelt at Gawain's feet, bowing his head so that the neck was plainly exposed. Gawain lifted the axe up high and brought it whistling down, severing the Knight's head cleanly from his shoulders.
As he passed Gawain, the head turned and regarded him solemnly. 'Remember your promise, Gawain. Meet me at the Green Chapel in one year and a day.' Then spurring his horse, the Green Knight galloped out of the hall.
Pretty cool, huh!!!!! Give this book a chance as it rocks in a major way!!!!!!
Well, not to overstay my welcome, I bid thee a most greatful adieu,


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Live the Moment
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Photo license: © All rights reserved
All too often we get caught up in the busyness of life, failing to tune into our surroundings and to appreciate what's there. It was a gorgeous June evening when I captured this image from the patio of the Italian farm house Fred and I call our current home away from home. At that time I was alone, Fred away on a business trip, but didn't feel the least bit lonely. In fact I felt euphoric, totally caught up in the beauty around me. Each time I look at this shot I not only relive that moment, but once again make an effort to slow down, breathe deeply, engage my senses, live the moment.
29 responses
Donald Garrett gave props (5 Jan 2011):
Beautiful simplicity!!
Warren ~Mangione~ gave props (5 Jan 2011):
Wonderful resolution, emotion and a great photo!
Patty Gross (Deleted) gave props (5 Jan 2011):
A fantastic scene from the farmland of Italy!! It would be pure bliss to live in such a peaceful place. Very well composed, Deborah! I truly love this photograph!!
Leslie Hunziker gave props (5 Jan 2011):
this makes me feel so warm!
Debrah Leonard (Deleted) gave props (5 Jan 2011):
so important....
Richard Knight gave props (5 Jan 2011):
Perfect image to accompany your sentiments.
Michaela K. gave props (5 Jan 2011):
so lovely, I wanted to spotlight this, but I was to late...
Andrea Petersen gave props (5 Jan 2011):
Very positive attitude, Deborah and what we should all aspire to do as a resoultion and the solution to enjoy life... Sometimes there is only that moment or chance to breathe in the beauty of what is around us as none of us knows what lies ahead ...Beautiful image and sentiments.
Regenia Brabham gave props (6 Jan 2011):
A wonderful resolution and a beautiful shot!!
Susan Littlefield gave props (6 Jan 2011):
A beautiful capture, and I agree with your sentiments! We may need to visit your area to experience this!!
Jim Ford (Deleted) gave props (6 Jan 2011):
Beautiful landscape....and add look closely.
Carol Arntsen Masiak gave props (6 Jan 2011):
words to live by...
Debbie Smartt gave props (6 Jan 2011):
I love this resolution! A very strong image!
elfriede fulda gave props (7 Jan 2011):
Lovely and a take to heart resolution Deborah
Lanis Rossi gave props (7 Jan 2011):
Sonia Adam Murray gave props (7 Jan 2011):
John Linton gave props (8 Jan 2011):
Rockin' the new year...
Joy B gave props (8 Jan 2011):
This is gorgeous and a desirable code to live by!
Michael Ball gave props (9 Jan 2011):
You have an excellent eye Deborah!! Beautiful and perfect resolution!!
Al Gieryna gave props (10 Jan 2011):
Perfect entry...seize that day, Deborah!
Maura Wolfson-Foster gave props (10 Jan 2011):
How marvelous.....Happy 2011 to you, and your's, Deborah ~ xox
Steve Baker gave props (11 Jan 2011):
Perfect shot for the sentiment - I remember days like this on the farm, but less so in town now. Still, the farm is only 5 minutes walk away...
Litz Go gave props (13 Jan 2011):
you live in paradise, my dear Deborah!
Sarah Dudley gave props (18 Jan 2011):
Ronnie Ginnever gave props (18 Jan 2011):
Very beautiful image, wonderful golden light.
Penny Nannini gave props (20 Jan 2011):
I love this one, it just feels good :)
Winky Vivas (Deleted) said (26 Jan 2011):
Absolutely beautiful! Wonderful colors, composition, and light. I really love your narrative here. Great job!
Judy and Wayne Wanamaker gave props (1 Feb 2011):
Simply stunning! The light is heavenly.
Donna Mullins (Deleted) gave props (17 Mar 2011):
love the title. great food for thought.lovely photo.


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Gene expression profiling of liver from dairy cows treated intra-mammary with lipopolysaccharide
© Jiang et al; licensee BioMed Central Ltd. 2008
Received: 26 March 2008
Accepted: 24 September 2008
Published: 24 September 2008
Liver plays a profound role in the acute phase response (APR) observed in the early phase of acute bovine mastitis caused by Escherichia coli (E. coli). To gain an insight into the genes and pathways involved in hepatic APR of dairy cows we performed a global gene expression analysis of liver tissue sampled at different time points before and after intra-mammary (IM) exposure to E. coli lipopolysaccharide (LPS) treatment.
Approximately 20% target transcripts were differentially expressed and eight co-expression clusters were identified. Each cluster had a unique time-dependent expression profile and consisted of genes involved in different biological processes. Our findings suggest that APR in the liver is triggered by the activation of signaling pathways that are involved with common and hepatic-specific transcription factors and pro-inflammatory cytokines. These mediators in turn stimulated or repressed the expression of genes encoding acute phase proteins (APP), collectins, complement components, chemokines, cell adhesion molecules and key metabolic enzymes during the APR. Hormones, anti-inflammatory and other hypothalamus-pituitary-adrenal axis (HPAA) linked mediators also seemed to participate in APR.
Performing global gene expression analysis on liver tissue from IM LPS treated cows verified that the liver plays a major role in the APR of E. coli mastitis, and that the bovine hepatic APR follows the same pattern as other mammals when they are challenged with LPS. Our work presents the first insight into the dynamic changes in gene expression in the liver that influences the induction, kinetics and clinical outcome of the APR in dairy cows.
Mastitis caused by Escherichia coli (E. coli) is a common disease in lactating dairy cows. During infection, lipopolysaccharide (LPS) released from the cell wall of E. coli rapidly induces a sophisticated inflammatory response. Locally it is characterized by recruiting leukocytes especially neutrophils to the injured mammary tissue, as well as activation of macrophages in the mammary gland to produce pro-inflammatory cytokines [1]. These pro-inflammatory cytokines include tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6), which have profound roles in the inflammatory response. Systemic consequences are observed as the acute phase response (APR) phenomena such as fever, leukocytosis and altered plasma concentration of acute phase proteins (APP). LPS is a highly potent activator of the pro-inflammatory response during E. coli infection and is often used to simulate the gram negative mastitis. It provokes secretion of above pro-inflammatory cytokines which in turn activate or suppress expression of acute phase genes in hepatocytes, vascular endothelium and other target cells [2, 3]. Although some extra-hepatic production occurs [4, 5], the liver is thought to be the major contributor to the APP in the blood. APR is also commonly accompanied by a widespread change of metabolism [6].
The contribution of the liver to the circulating levels of cytokines and APP in vivo in cattle is not fully understood. In cattle, in vitro studies using Kupffer cells (liver macrophages) have shown an important contribution of liver to the cytokine production [7]. In vitro, recombinant bovine cytokines can stimulate the secretion of haptoglobin (HP) from bovine hepatocytes [8]. Furthermore, synthesis of serum amyloid A (SAA) was shown to be mainly regulated by TNF-α, IL-1, IL-6 and glucocorticoid via a network of transcription factors and antagonists [9]. Our previous study shows that liver has a great capacity to produce both pro- and anti-inflammatory cytokines and APP when dairy cows are infused LPS intra-mammary (IM) [10]. All these studies suggest a strong connection between cytokines activity and APP production in cattle during inflammation.
The biological functions of APP such as HP and SAA in the APR are only partly known. Although the APP is highly conserved during evolution [11], their presence and importance differ among animal species. The significantly elevated APP expression in liver and increased concentration in blood and milk during udder infection indicate a crucial role of these proteins in the host's anti-pathogen response to mastitis [10, 12, 13]. Other genes involved in the energy metabolism, integrity and function of hepatocytes are also expected to be regulated in the liver during the APR, and may influence the induction, kinetics and clinical outcome of the APR to LPS induced inflammation in dairy cows [3].
To gain a better insight into the genes and pathways involved in hepatic APR in dairy cows, we performed a global gene expression analysis of liver tissue sampled at different time points relative to intra-mammary exposure to LPS treatment.
This study presents the first global gene expression profiling of the hepatic APR in dairy cows following intra-mammary exposure to LPS. Clinical findings (e.g. fever, high somatic cell count (SCC) in the milk, temporary leukopenia, leukocytosis) verified the induction of mastitis inflammation by LPS. The bovine array data strongly support that the hepatic APR is triggered by the activation of the signaling pathways that are involved with common and hepatic-specific transcription factors and pro-inflammatory cytokines. These mediators in turn stimulates or represses the expression of genes encoding APP, collectins, complement components, chemokines, cell adhesion molecules and key metabolic enzymes during APR. Hormones, anti-inflammatory and hypothalamus-pituitary-adrenal axis (HPAA) linked mediators also seem to participate in the modification of APR. The alteration of these acute phase genes is thought to be responsible for the observed systemic APR phenomena.
The overall gene expression pattern of the hepatic APR in dairy cows depicts what has been found in other animal LPS models such as mice, canine and sheep using different LPS application routes. In the liver of mice given intraperitoneal (i.p.) LPS there was an up regulation of genes involved in defense and immunity, and intracellular signaling while genes involved in metabolism of fatty acid, bile acid and cholesterol were down regulated [3]. Similar findings in the liver of intravenous (i.v.) LPS-challenged sheep [14, 15] and canine [16] suggests that the changes in the gene expression we have in the bovine liver during APR is a common response in mammalian species. In the following sections, changes in individual gene expressions in LPS challenged dairy cows will be discussed in relation to the existing and general knowledge of the hepatic APR.
Initiation of APR in liver
LPS IM infusion induces systemic APR, which in the liver is associated with the pro-inflammatory cytokines produced by the activated macrophages. CD14 and TLR4 are the cell membrane proteins which bind LPS and may trigger the signaling pathways of macrophages activation [17, 18]. TLR2 also has a recognizable role in the inflammatory activation of liver shown in mice by i.p. LPS treatment [19]. TLR2-MYD88 elicits the signaling cascades that activate AP1 and NFKB [20]. The latter promotes the transcription of IL-6 and TNF-α [21, 22]. We observed that the expressions of TLR2, MYD88, AP1 and p65 subunit (NFKB3) of NFKB complex, its inhibitor (NFKBIA), both IL-1α and IL-1β, and IL-1 receptor antagonist IL1RN were all up regulated. Although the increased expression of IL-6 and TNF-α is less dramatic when measured using the microarray, it is significant using Q-PCR [10]. Increased expression was also observed in the cytokine receptors like TNFRSF1A and TNFRSF5 (CD40), and the component of receptor pathway such as TOLLIP, which is an essential element of the IL-1 receptor signaling pathway [23].
Hormones are another family of key mediators involved in APR. We found a decreased expression of prolactin receptor (PRLR), similar to that shown in hepatic tissue after i.p. LPS injection [24]. PRL induced STAT3 [25] which however was up regulated in our study. One explanation is that in the liver STAT3 is also inducible by growth hormone (GH), IL-6 family cytokines and epidermal growth factor (EGF) [26, 27], and we did observe increased expression of the EGF receptor. Activated STAT3 can enhance the transcription of C/EBPs and AP1, which both are important transcription factors controlling the production of APP and other inflammatory genes such as SCARB1 that participates in the clearance of LPS in the liver [28–30]. We observed an elevated expression of C/EBPδ, AP1 and SCARB1. In addition, SOCS2 and SOCS3, the attenuator of STAT3 mediated signal transduction, were also altered.
It seems APR predominantly relies on the activation of signaling pathways involved with transcription factors and pro-inflammatory cytokines. And it may subject to the modification by the hormones via the corresponding receptors. The coordination of pro- and anti-inflammatory mediators such as inhibitors or antagonists also participated and may provide the host with a state of "high alert" in which the immune response can be efficiently initiated if the infection progresses, and restrained if the infection is resolved or becomes excessive or prolonged.
HPAA and APR in liver
Fever was observed in the cows after LPS challenge and as a physiological stress, it can lead to the change of serum cortisol levels. Prostaglandin (PG) E2 has a profound role in thermoregulation during infection [31]. In LPS-induced fever, first febrile phase (~0.5 hour post LPS) is triggered by the activation of PGE2 synthesis mainly in macrophages in liver and lung [32]. PGE2 is synthesized via the cyclooxygenase pathway in which COX-2 is the key enzyme. We found increased expression of COX-2 in the liver within 3 hours after LPS IM infusion. In fact, APR represents a cross-talk between the peripheral inflammation and central nervous system [33]. During infection HPAA is activated by pro-inflammatory cytokines and influences the inflammation by releasing glucocorticoids such as cortisol [15, 34]. The majority of cortisol in serum is bound to CBG and is biological inactive [35]. We observed a decreased expression of CBG in the bovine liver and studies of human HepG2 cell in vitro showed that pro-inflammatory cytokines IL-1 and IL-6 can decrease CBG synthesis [36]. The expected outcome is increased levels of unbound (active) cortisol in serum in response to the local inflammation in the mammary gland. It suggests that HPAA linked mediators (cortisol and PGE2) may respond to the expression change of acute phase genes in liver.
Expression of APP
APP is one of the most important products of the liver during the infection. We observed that the expression of major positive APP such as SAA3, HP and AGP were all up regulated. No significant change was observed in C-reactive protein, shown to be less responsive in cattle as compared to human, pigs and dogs during the acute infection [4]. SAA, HP and AGP are class I APP which dependent on IL-1-NFKB signaling pathway and contain C/EBP binding sites in their promoter regions [37]. In our study C/EBPδ was elevated while C/EBPα was decreased less than 3 hours after LPS treatment confirming the inverse relationship between these transcription factors during APR [38]. STAT3, a class II acute phase gene (induced by IL-6), was also up regulated is known to stimulate the transcription of C/EBPs as described previously and thereby affecting expression of the APP indirectly. The increased expression of common (NFKB, STAT3) and hepatic-selective (C/EBPδ) transcription factors suggests that the expression of APP is controlled by a network of transcription factors involving both classes of the acute phase genes.
It is also notable that some APP from the complement pathway or collectin family, for example MBL, was down regulated. Studies in humans have shown that IL-1 can suppress the MBL expression [39]. Another example is C4BPB, a crucial regulator in both the classical and lectin pathways. Expression levels of up stream regulators of C4BPB such as HNF-3 and PFKFB2 [40, 41] were suppressed, which may explain the decreased expression of C4BPB we observed in the bovine liver during the APR. Complement components (CC) and collectins are considered as the first line of defense against pathogen invasion. However in our study, LPS was infused into the mammary gland and decreased expression of collectins and CC may be due to the absence of direct binding to the foreign surface antigen.
Leukocytes recruitment and activation
Leukocytosis was observed in the LPS treated cows and correspondingly we also observed an increased expression of genes, which play a key role in leukocytes migration and activation in the liver. Among those genes are 1) chemokines: macrophage inflammatory protein (MIP-3α/CCL20), monocyte chemotactic proteins (MCP-1/CCL2, MCP-2/CCL8) and neutrophil-activating protein NAP-3/CXCL1; 2) cell-adhesion molecules (CAM): ICAM-1; and 3) other cell surface molecules and colony stimulating factors: CD97 and CSF-1. CD97 plays an essential role in migration of leukocytes particularly neutrophils [42], and it is a marker of early lymphocyte activation [43, 44]. CSF-1 is central to the regulation of production, maintenance and function of macrophages [45]. NFKB and MYD88, which are responsible for inducing the expression of many of the above mentioned chemokines and CAM [46–50], were also up regulated suggesting their crucial role in hepatic leukocytes migration and activation.
Metabolism and APR
A large group of metabolism genes were down regulated in the bovine liver in response to IM LPS infusion. Of particular interest was the increased expression of PRKAB1, a regulatory subunit of AMP-activated protein kinase (AMPK), as recent findings show that AMPK plays a major role in the control of hepatic metabolism [51]. Activation of AMPK leads to inhibition of glucose production and we did indeed observe decreased expression of the gluconeogenic key enzymes PCK1 and G6PC and their transcriptional co-activator PGC-1α [52]. Moreover, down regulation of the expression of PCK1 and G6PC by STAT3 [53] whose expression levels was increased in our study, indicates that the hepatic metabolism may also be influenced by transcription factors from the signaling pathways in APR. Signaling gene networks were also shown to be coordinately regulated in the liver of periparturient cows in response to ketosis [54]. In general our results does support the findings that the inflammatory response affects metabolism in dairy cows [55].
Performing global gene expression analysis on liver tissue from IM LPS treated cows verified that the liver plays a major role in the APR of E. coli mastitis, and that the bovine hepatic APR follows the same pattern as other mammals when they are challenged with LPS. Our work presents the first insight into the dynamic changes in gene expression in the liver that influences the induction, kinetics and clinical outcome of the APR in dairy cows.
Animals and LPS treatment
The experimental design has previously been reported [10]. In short eight healthy, high yielding (38 kg milk per day) Holstein-Friesian dairy cows in their first lactation (9 to 12 weeks after calving) were chosen for this study. The udder health of the cows was evaluated based on somatic cell count (SCC) and bacteriological examinations. All cows had SCC < 100,000 on both front quarters and SCC < 138,000 on both behind quarters, and were free from mastitis pathogens. Sterile polyvinyl catheters (Micro-Renathane) were inserted into the jugular vein of the cows. The catheters were flushed with a sterile 0.9% NaCl solution containing 50 IU/ml Na-heparin (Loevens Kemiske Fabrik, Ballerup, Denmark). At time 0 the right front quarter was infused with 200 μg E. coli LPS (0111:B4) (Sigma-Aldrich, Brøndby, Denmark) dissolved in 10 ml 0.9% NaCl solution, the left front quarter serving as control was infused with 10 ml 0.9% NaCl solution. Production data, clinical signs as well as blood and milk parameters associate with LPS treatment were recorded throughout the experiment. The clinical and paraclinical findings verified the induction of mastitis inflammation by LPS. LPS intra-mammary infusion induced fever and a high SCC in the milk of the treated quarter. Further, increased concentrations of TNF-α, SAA, HP were found simultaneously with temporary leukopenia followed by leukocytosis and all characteristic observations in an LPS IM infusion model. Quantitative PCR also confirmed the high expression of cytokines TNF-α, IL-1β, IL-6 and IL-10 and the acute phase proteins SAA3 and HP in the liver following IM LPS infusion [10].
All procedures involving animals were approved by the Danish Animal Experiments Inspectorate and complied with the Danish Ministry of Justice Laws concerning animal experimentation and care of experimental animals.
Liver biopsies
Liver biopsies were taken at -22, 3, 6, 9, 12 and 48 hours relative to LPS infusion in 4 cows, and also at -22, 9 and 48 hours in the remaining 4 cows. Sampling procedures for liver biopsies were as previously described [56]. A control study using cows infused with 0.9% NaCl showed that there was no effect of taking the biopsy, neither in the clinical measurement nor in the expression of a selected subset of genes [10]. Therefore, only 36 samples taken from the LPS treated cows were measured for the gene expression using microarrays.
RNA purification and microarray processing
Isolation and labeling of RNA and microarray processing were performed as described elsewhere [57]. RNA from liver biopsies was isolated using Trizol Reagent (Invitrogen, Taastrup, Denmark). Five μg RNA was labeled using the SuperScript Choice System (Life Technologies) according to the manufacturer's instructions, except for using an oligo-dT primer containing a T7 RNA polymerase promoter site. Biotin labeled cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit (Enzo, Farmingdale NY, USA). 15 μg of cRNA was loaded onto the probe array cartridge of Bovine Genome Array (Affymetrix Clara CA, USA). The array contains 24128 probe sets which represent 15264 UniGene (annotation from May, 2006) to measure the global transcripts. Hybridization mixture of each sample (a specific cow at a certain time point) was loaded to an array resulting in a total of 36 arrays.
Bovine Genome Array annotation is available from the NetAffx. Genes and gene products attributes are characterized with the Gene Ontology (GO). However, in NetAffx (December, 2005) only ~16% of probe sets on the array were GO annotated. Additional annotation was obtained from the Ensembl database, using the biomaRt package in R [58] as the query interface. The query IDs (Ensembl gene ID, Entrez gene ID or Affy probe set ID) were extracted from the NetAffy annotation. Besides bovine genes, we also retrieved the annotation based on gene sequences of the human ortholog in Ensembl. From above resources ~48% of the probe sets on the array were able to be annotated with GO.
Statistical analysis
The data was analyzed using R (version 2.4.1), a programming language and development environment for statistical computing and graphics. Normalization of expression values was performed using the GeneChip Robust Multi-array Analysis (GCRMA) algorithm [59, 60]. In this algorithm, raw intensity values are background corrected based on a model using sequence information followed by quantile normalization [60, 61]. This algorithm combines the strength of stochastic-model based algorithms and physical models and has been shown to be superior in accuracy and precision to other normalization methods such as MAS, RMA and PerfectMatch [62]. The microarray data has been deposited in the Gene Expression Omnibus database (accession GSE10695).
Differential expression of individual genes was assessed using linear modeling and empirical Bayes methods [63] as implemented in the R package Linear Models for Microarray Analysis, LIMMA [64]. The linear models allows for general changes in gene expression between successive time points (3 to -22, 6 to 3, 9 to 6, 12 to 9 and 48 to 12). Each transcript targeted by the probe set was tested for the expression change between all pairs of the successive time points using a modified t-test, and for multiple contrasts using a moderated F test. The residual standard deviations are moderated across the probe sets to ensure more stable inference for each transcript. The moderated standard deviations are a compromise between the individual transcript-wise standard deviations and an overall pooled standard deviation. Since 24128 transcripts were measured by the array and tested for the alteration simultaneously, the multiple-tests adjustment is introduced to control the number of false positives. The multiple testing was accounted for using the Bonferroni method. Transcripts were deemed differentially expressed (DE) if one or more adjusted P values for the moderated t-test was below 0.01. The adjusted moderated F statistics also showed that all DE transcripts, except [GenBank: CK775003] (the probe set is Bt.21216.1.S1_at), were significant.
Gene set enrichment
There are 1789 UniGenes possessing Gene Ontology (GO) among all DE transcripts in contrast to 6843 UniGenes from the whole array. Within each co-expression cluster, overrepresentation of gene sets defined by the Biological Process (BP) of GO was tested using Fisher's exact test.
adenosine monophosphate-activated protein kinase
acute phase proteins
acute phase response
adenosine 5'-triphosphate
biological process
cell-adhesion molecules
complement components
- cRNA:
complementary ribonucleic acid
differentially expressed
- E. coli :
Escherichia coli
epidermal growth factor
GeneChip robust multi-array analysis
growth factor
gene ontology
hypothalamus-pituitary-adrenal axis
- i.p.:
international units
- i.v.:
linear models for microarray analysis
microarray suite
monocyte chemotactic protein
macrophage inflammatory protein
neutrophil-activating protein
prolactin receptor
quantitative polymerase chain reaction
robust multi-array analysis
somatic cell count
The work is financially supported by Danish Agricultural, Veterinary Research Council (23-04-0192); the European Commission, within the 6th Framework Programme, contract NO. FOOD-CT-2006-016250 and BIOSENS, a project collaboration between Lattec I/S, the Danish Cattle Federation and the Faculty of Agricultural Sciences, financed by 50% by the Directorate for Food, Fisheries and Agri Business.
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Tag Archives: Anna Hazare
Hazare gives clarion call to people to join Jan Sansad in August
Agencies, Jalandhar Social activist Anna Hazare today gave a clarion call to the people of Punjab to join the Jan Sansad at Delhi being organised after five months from today to support his campaign against corruption.
Addressing a rally organised...
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India Against Corruption (IAC) leader Arvind Kejriwal today said he is a 'dengue mosquito' and would bite both the Congress and the BJP.
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Guwahati, Nov 7
Reaffirming that there is not divide between him and Arvind Kejriwal, veteran Gandhian Anna Hazare today said that he will urge public to support people like Kejriwal and send 'good people' to Parliament.
No differences with Kejriwal, says Anna Hazare
New Delhi, Oct 1
Veteran social activist and anti-graft crusader Anna Hazare today said he would support India Against Corruption (IAC) member Arvind Kejriwal if the latter fights an election and said there were no differences between...
Anna to meet supporters, activists in Delhi
New Delhi, Sep 30
A social activist and anti-corruption crusader Anna Hazare reached here today to chalk out future course of action for his movement against corruption.
Mr Hazare during his two-day visit to Delhi would meet his supporters...
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In an apparent move to float a political party, social activist Anna Hazare today dissolved his team, popularly referred to as Team Anna, saying ''its work is over'' and decided not to have any more talks...
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New Delhi : Giving a call for 'sampurna kranti' (total revolution), veteran social activist Anna Hazare and his team today ended their fast for a strong Jan Lokpal and announced the formation of an ' alternative' political...
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''A wave of protest against...


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Monday, September 08, 2008
A Few Loose Ends Monday - CS4 Coming & Speeding Up LR2
Last week, while attending Photoshop World Convention is Las Vegas, I had the opportunity to meet (ever so briefly) Martin Evening, one of this year's inductees into the Photoshop Hall of Fame. Martin is an outstanding fashion photographer - check out his site here, but more than that, he is a Lightroom guru as well.
He just released his new Lightroom 2 book and runs Lightroom-News.com [link]. I mention this because Martin's site is a treasure trove on Lightroom info, work-a-rounds- and fixes. For instance, your Adjustment Brush running just a bit too slow in LR2 under Windows XP - here is the link to a possible fix I found on his site. Wait, there's more. Want to see Martin's Lightroom 2 photo-shoot - here is the link. Anyway, it stuff like this that makes his site one to add to your reader.
p.s. Thanks to David for pointing out that I had Martin incorrectly listed as winning the Guru Award.
3 comments:
- Arne Hofmann7:15 AM, September 09, 2008
Martin Evening's new Lightroom book, which I'm just reading, is also very good. It's clearly aimed at professionals, semi-professionals, or seriously advanced amateurs (but Lightroom's learning curve is not that steep anyway). You can learn Lightroom from pretty much any book, but Evening's has far more technical detail than most, and I like to understand why what I'm doing is working (it helps me to remember as well as to understand new developments...).
ReplyDelete - David L. Robertson2:41 PM, September 09, 2008
Actually Martin was not a Guru winner. Instead, he was inducted into the Photoshop Hall of Fame, a much higher honor and richly deserved. I had the occasion to communicate with him during the writing of his first book on Lightroom and found him to be very personable, approachable and helpful.


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Lot 355: Ingrid Bergman Vintage SP (Casablanca)
Rare Autograph, Manuscripts & Book & Aviation Auction
by One of a Kind Collectibles Auctions, LLC
27 October 2016
Miami, FL, USA
Past Lot
Looking for the realised and estimated price?
Description: Attractive glossy 3.5 x 5.5 promotional German postcard photo of Bergman for Casablanca by Rudel-Verlog, signed in black ink. In fine condition, with a small crease to upper left corner portion, otherwise fine.
Notes: To place a bid go to www.oneofakindauctions.com


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The original play follows several morally bankrupt characters through a series of sexual encounters and traces the effects of each disconnected encounter down a causal chain of destruction. As one person is wronged they, in turn, corrupt another until all of the characters are as lost as the originator of the series.
LaChuisa's version takes this idea a step further by placing each of the ten scenes in different time periods. The dramatic cohesion of the show is maintained by the presence of the Whore. It is she who begins the process and it is she who inadvertently prompts the character of the Senator to attempt an end. Perhaps the Whore's most striking appearance throughout the show takes place in Scene 5. In this scene a Young Wife is basically forced into sexual intercourse by her husband and, while this is taking place, fantasizes about a man named Tom who she had a brief dalliance with. As she sings of her past exploits, the wife slides from beneath her husband and dances a surreal pas de deux through a mirror with a dark silhouette who the audience can barely make out as the Whore. It is a poignant moment that beautifully demonstrates the duality present, to some degree, in all of our lives. The final scene of the show takes place after a sexual encounter between the Senator and the Whore. Most of the scene is devoted to the Senator's song chronicling the confusion and emptiness he feels as a result of his past experiences. Indeed, it is not until she wakes up next to him that he remembers who he had spent the night with. The Senator, filled with regret, attempts to leave but is beckoned back to the bed as the Whore echoes the words that began the play. As the scene freezes in a tableau every character from the show appears onstage with a haunting repetition of the words "hello again" as they circle each other, still in search of the love that eludes them.
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Viewing Issue Advanced Details [ Jump to Notes ] | nemesis.cpp | ||||||||||||
ID | Category [?] | Severity [?] | Reproducibility | Date Submitted | Last Update | ||||||||
03538 | Sound | Minor | Always | Nov 19, 2009, 04:08 | Dec 15, 2011, 18:59 | ||||||||
Tester | Ace | View Status | Public | Platform | MAMEUI | ||||||||
Assigned To | Fujix | Resolution | Fixed | OS | |||||||||
Status [?] | Resolved | Driver | nemesis.cpp | ||||||||||
Version | 0.135 | Fixed in Version | 0.144u4 | Build | |||||||||
Summary | 03538: salamand, lifefrce, lifefrcej, salamandj: Music is too quiet in Salamander and Lifeforce | ||||||||||||
Description |
I couldn't help but notice that the music in Salamander and Life Force plays WAY too slowly compared to the original arcade board and the port in Salamander Deluxe Pack Plus for the PlayStation and Sega Saturn. I've attached a recording of Poison of Snake from both Salamander in MAMEUI v0.135 and Salamander in Salamander Deluxe Pack Plus(PlayStation version) for comparison - first the MAME sound, then the Salamander Deluxe Pack Plus sound. The music is also too silent in Salamander and Life Force, and adjusting the volume between the different sound chips doesn't really fix the problem, since some sound effects also come out too loud(the music is produced by the YM2151). Also, as you can tell by the recording, the Stereo in MAME is backwards; the notes that come out the left speaker comes out the right speaker and vice-versa. |
Steps To Reproduce | |||||||||||||
Additional Information |
The music speed issue was moved to 03737. The reverse stereo issue was moved to 04565. |
Flags | Verified with Original | ||||||||||||
Regression Version | |||||||||||||
Affected Sets / Systems | salamand, lifefrce, lifefrcej, salamandj | ||||||||||||
Attached Files
Poison of Snake - MAME and Salamander Deluxe Pack Plus.mp3 (2,836,242 bytes) Nov 19, 2009, 04:08 | ||||||||||||
PowerofAnger_OST.ogg (1,279,393 bytes) Nov 19, 2009, 19:22 | |||||||||||||
Relationships | |||||||||||||||||||||
Notes | |
Fujix (Administrator)
Nov 19, 2009, 17:36
It is an old problem that MAME plays music way too slower than the arcade. Probably we need an original recording to determine the SFX volume. One thing I concern about is, as you mentioned, some SFX are played by YM-2151 and others are by K007232, but I don't know if the original has something special to control one of eight YM-2151 output volumes only for SFX, and MAME doesn't hook it up yet. IMO, it is unlikely. |
Fujix (Administrator)
Nov 19, 2009, 19:06
edited on: Nov 19, 2009, 19:23
Confirmed speed and channel issues. I attached OSD music clip for reference. Still asking for opinions on SFX volumes. |
Ace (Tester)
Nov 19, 2009, 20:03
edited on: Nov 19, 2009, 20:05
I'm just saying it's too loud since the sound effects produced by the YM2151 drown out the music. Whenever a sound plays, the music is practically inaudible. I'm not sure if it's the same on an actual Salamander or Life Force cabinet, since I have no experience with the original arcade boards, but when music or sound effects don't seem to have a proper sound balance, it sounds bad to my ears. Anyone know if the volume balance between sound effects produced by the YM2151 and the music is correct in MAME? |
R. Belmont (Developer)
Jan 16, 2010, 23:33
I'd like to get a recording from a real PCB if possible. Console ports are never valid sources for bug reports. |
Ace (Tester)
Jan 22, 2010, 01:02
edited on: Jan 22, 2010, 01:03
If I'd have had a real Salamander PCB, I'd record everything off that. A little note: I noticed that the Japanese Life Force actually has better sound balance between music and sound effects, but in Salamander and the US Life Force, the music is drowned out by the sounds. Is that the way it should be or is one of them wrong? Plus, if you listen to the Salamander OST, you can clearly tell the music is faster and the Stereo is reversed. |
gamestone (Tester)
Feb 15, 2010, 20:49
edited on: Feb 16, 2010, 17:21
I have two Salamander boards (one Japanese and one UK, only the Japanese one works) so I can make a recording later this week but my machine is only wired for mono from the JAMMA connector, the stereo output comes from another output connector on the board and I have no cable for that connection. I have a hires scan of the Japanese Operator's Manual on my website (Gradius Home World), which may have info you need as well as other Gradius related manual scans. http://www.gamestone.co.uk/gradius/downloads_hi_res_scans.php EDIT: I'm in the process of making the stereo loom so will try to record the stereo source. One problem maybe the two pots for the different left and right volumes but I'll see how it goes in getting the balance right. |
Ace (Tester)
Feb 16, 2010, 23:25
Oh, hey GameStone. Please provide a recording of your Salamander arcade board. We'll forget about the reversed Stereo for now, I'd like to focus on the speed of the music and the balance between sound effects and music. |
Fujix (Administrator)
Feb 17, 2010, 02:55
edited on: Feb 17, 2010, 03:22
That's really cool collection of Konamish games. I'm really enjoying it. According to the page 14 of the manual, "(4) CN2/CN3" is "Stereo/Monaural Switching Connector". This may help recording. BTW, I found some typos and mistakes of Jp texts in the hires scan page, here's fix: http://www.e2j.net/jptext.txt Also I uploaded the latest version of original Japanese game descriptions with Kana readings for MAME which I maintain: http://www.e2j.net/jpdescriptions.txt I hope they will help. |
gamestone (Tester)
Feb 17, 2010, 18:04
It was sunny when I got home today so I was inspired to sort this out today rather than the weekend. After making the very dodgy cable at work from a CDROM audio cable (it fits the four pin stereo connector on the PCB) and a couple of phono connectors. MONO and STEREO. You move the jumper block from CN3 MONO to CN2 STEREO which then routes the audio to the CN5 connector rather than the JAMMA connector, you then connect the new cable to the CN5. Pin-outs included on page 19 of the Operator Manual (and in the arcade board rar below). I have checked and the Left and Right channels are the right way round in the recording, I tried to get the balance more or less the same between the left and right channel (two pots on the PCB), which was not that easy as the pots are not the most accurate I've seen. Stage 1 and 2, I died on the Tetren One thing I must say the sound FX are quite a bit louder than the music. Some pictures of the setup http://www.gamestone.co.uk/mame/salamander_arcade_board.rar Original stereo wav versions - 16meg & 32meg http://www.gamestone.co.uk/mame/salamander_attract_mode.rar http://www.gamestone.co.uk/mame/salamander_stage_1_and_2.rar mp3 VBR 320kbps version - 3.7meg & 8.5meg http://www.gamestone.co.uk/mame/salamander_attract_mode.mp3 http://www.gamestone.co.uk/mame/salamander_stage_1_and_2.mp3 I've added the original wav versions just in case you need the uncompressed file to view the waveforms. Cheers for the typos text, I'll be changing them soon as that section is going to have a revamp anyway. |
Fujix (Administrator)
Feb 17, 2010, 19:44
edited on: Feb 17, 2010, 19:46
Thank you for important recordings. Reverse stereo channel in MAME has been proved to be a bug. I estimated the correct volume proportion with a wav analyzer based on RMS and peak levels. To simply reproduce the wav recordings, - YM2151 is 205% of current MAME volume (1.00 x 205% = 2.05) - K007232 is 180% of MAME (0.10 x 180% = 0.18) - VLM5030 has not small difference from the original, they should be 320 - 350% for convenience (0.60 x 335% = 1.89) Normalized values: - YM2151: 1.50 - K007232: 0.14 - VLM5030: 1.47 |
Ace (Tester)
Feb 18, 2010, 01:22
After hearing that, I'd like to touch on some other sound issues: -There appears to be some sort of filtering on the VLM5030 that MAME doesn't emulate, as the speech comes out "cleaner" in MAME than on the original arcade board -Some notes from the YM2151 seem to come out wrong compared to the original arcade board. It's strange how Salamander's sound effects are so loud when the Japanese Life Force has better balance between the music and sound effects. Is that because the ROM is programmed differently or is it a bug in MAME? I can tell you that the US Life Force sounds like Salamander in MAME, but the Japanese Life Force has quieter sound effects, making for a better balance between sound effects and music. |
Fujix (Administrator)
Feb 18, 2010, 06:22
edited on: Feb 18, 2010, 06:31
Ace, if you find something new, please make a new bug report thread. It is not a good idea to put everything into one entry. And for the Life Force issue, the US version of Life Force is a simple modification of Salamander for the US market. The Japanese version of Life Force is a sequel of Salamander with a lot of updates including graphic mods for US LF. But for the sound volume, we can't do anything until someone makes the original recordings available JUST AS GameStone kindly did. Regarding sound balance issue, I won't accept any kind of possibility or guessing without original reference, it is already mentioned in the Rules page at http://mametesters.org/rules.html |
Fujix (Administrator)
Dec 15, 2011, 18:59
edited on: Dec 15, 2011, 19:01
Updated the volume proportion based on the PCB recordings in line with the VLM5030 update, although VLM5030 is still missing a low pass filter. |


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Supreme Team's E-Sens Criticizes Comedians Doing Hip Hop
Supreme Team's E-Sens has a bone to pick with the increasing number of comedians making attempts into the hip hop genre.
On November 27, the Supreme Team member tweeted, "Why is it that when [comedians] have no ideas or have nothing to do, they always try and imitate hip hop musicians with "music" that aren't even funny? Truthfully speaking, I am f*cking tired of seeing this."
Though the tweet wasn't directed at anyone in particular, with the rise of such cases through efforts from Jung Hyung Don, Brave Brothers, UV and more, the tweet was seen as an indirect diss at them.
His agency later commented in on the manner as well saying that the tweet was an expression of E-Sens' personal opinion and did not reflect the opinion of the agency itself.
The tweet brought divided opinions from netizens with some agreeing saying things like, "It's an embarrassment to true hip hop artists", while others shared, "Then hip hop musicians shouldn't come out on variety programs either."
What's your take on the matter?
Photo Credit: Online community
- Tag
- Supreme Team 20121127


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There is something to that thought while riding... You've always said... "Rain? What's a little rain gonna do?" :D of course I would always be heading to a shower and towels... And not back on the bike for hours! By the way... I feel like I'm on your shoulder because of this blog! I keep checking to find out how "our" ride was today! <3
Day #16 Ochoco Lake CP to Dayville, OR
Trip Start
Jul 17, 2013
Trip End
Aug 14, 2013
The eastern Oregon citizens take their land rights seriously and I was warned by a ODOT work not to try and bushwhack off the Hwy. or I'd likely get into trouble. Luckily in Mitchell I met Daniel from Germany he was traveling west on the TransAm route and had almost completed his transcontinental journey. He advised me to push on to Dayville and stay at the Presbyterian Church there. The church has been providing shelter and amenities to traveling cyclist since the inception of the TransAm route in 1976. Great digs, with showers, kitchen, washer/dryer, and even internet. At the Church I met Ariel and Bethany from Maryland they too are west bound cyclist almost finished with there journey. They had started on May 17th.
Thoughts while riding: If I'm already soaked in sweat when I put on my rain gear what am I protecting myself from? More water?
Distance: 78 miles
Ride Time: 6:00 Real Time: 7:30
Vertical Feet: 3900