text:
Kevin quick murder: Two plead guilty to February slaying
CHARLOTTESVILLE, Va. (AP) - Two people have pleaded guilty to charges stemming from the slaying of a Waynesboro reserve police officer.
Leslie Hope Casterlow of Manassas and Anthony Lee White of Louisa are among nine people who were indicted on federal charges in the case.
Online court records show Casterlow pleaded guilty to obstruction of justice on Wednesday in federal court in Charlottesville. A statement of facts says Casterlow disposed of evidence in the slaying of 45-year-old Kevin Quick and a home invasion robbery.
White pleaded guilty to a racketeering conspiracy charge.
Quick was reported missing Feb. 1 after he failed to show up to visit his girlfriend and their 4-month-old daughter. His body was found days later in a wooded area northwest of Richmond.


text:
by Dr. Don Kuehle
We mourn the tragic shooting of 49 people in Orlando, Fla. As Christians, we hold that all life is sacred because every life was created by a holy God. Every life has value, regardless of a person's beliefs or lifestyle.
The tragedy is that the lives of these 49 people was cut short by an act of violence. The killer believed in a god-of-death; his religious
Please subscribe or log in to access full content.

subset: commoncrawl


text: The Scout features the Matrix camo pattern and is made of polyester which is UV coated for fade-free usage. The Scout weighs only 12 lbs. and features a spring steel frame. FeaturesDimensions: 62" Tall x 34" Wide x 54" Deep, Doors and Windows are zippered on each side with 2 large openings in the front, 2 Interior Gear Pockets, Carrying Case with Attached Back Pack Straps, Phantom Pro black interior liner for added concealment, Zippered Entrance and Two Zippered windows on each side with 2 large openings in front, Windows and large front opening lined with optional shoot-through mesh, Deluxe Folder Up Chair wtih backrest included: Chair is 24" Wide x 20" Deep


text:
The US Office of the Comptroller of the Currency, the Federal Reserve Board and the FDIC issued a proposal to remove two items from Schedule B of form FFIEC 101, the report entitled Risk-Based Capital Reporting for...more
FDIC bank examinations generally include a focus on the IT systems of banks with a particular focus on information security. The federal banking agencies issued Interagency Guidelines Establishing Information Security...more
Throughout the past several years, data privacy and security practices have evolved into more than just defending against identity theft and protecting sensitive data. In fact, since 2014, to help raise awareness for data...more
In December 2016, Thomas Curry, the Comptroller of the Currency, stated that cybersecurity was the single greatest systemic threat to our financial system. He was not being hyperbolic. Cybersecurity should be on...more
SEC/CORPORATE - President-Elect Trump Nominates Clayton as SEC Chair - On January 4, President-elect Donald Trump announced his nomination of Jay Clayton to serve as chair of the Securities and Exchange Commission,...more
On November 28, 2016, the Consumer Financial Protection Bureau (the "Bureau") issued CFPB Compliance Bulletin 2016-13, titled "Detecting and Preventing Consumer Harm from Production Incentives" (the "Bulletin"). Inviting...more
The Office of the Comptroller of the Currency has indicated in a recent bulletin that its examiners will gradually incorporate a Cybersecurity Assessment Tool into its examinations of national banks and other institutions...more
Massachusetts Voters Approve Legalization of Marijuana for Recreational Purposes - Massachusetts voters have approved a ballot initiative legalizing the use of marijuana for recreational purposes, which will become law...more
Editor's Note - A Possible Blueprint for Financial Regulatory Reform in the Trump Administration. During his campaign, President-elect Trump consistently emphasized that financial regulatory reform is a critical...more
The Federal Financial Institutions Examination Council (FFIEC), whose members include the CFPB, has finalized guidance setting forth a revised uniform interagency consumer compliance rating system (CCRS). The revisions...more
On October 19, 2016, the Federal Reserve, the Federal Deposit Insurance Corporation and the Office of the Comptroller of the Currency (together, the "Prudential Regulators") published an advance notice of proposed rulemaking...more
Last week, FinCEN (Financial Crimes Enforcement Network) issued a formal Advisory to Financial Institutions and published FAQs outlining specific cybersecurity events that should be reported through Suspicious Activity...more
More regulators (apart from the FTC) are now taking note of cybersecurity issues in the financial services industry and are taking steps to protect the industry and its consumers. Earlier this year, the Consumer...more
On October 17, 2016, the Federal Financial Institutions Examination Council ("FFIEC" or the "Council") released a set of answers to frequently asked questions about its cybersecurity assessment tool (the "CAT"). The FFIEC, an...more
On October 18, 2016, the Federal Financial Institutions Examination Council (FFIEC) issued answers to frequently asked questions (FAQs) to clarify points in FFEIC's Cybersecurity Assessment Tool (Assessment). FFIEC released...more
On October 17, the FDIC released revised interagency Military Lending Act (MLA) examination procedures for use in connection with consumer credit transactions occurring on or after October 3, 2016. The revised procedures...more
Three federal banking agencies have announced plans to develop new rules that would establish cyber risk management and resiliency standards for large interconnected entities under the agencies' supervision, as well as those...more
The new cybersecurity rules proposed by the New York State Department of Financial Services require financial services institutions to have extensive cybersecurity protections in place; including cybersecurity programs,...more
On October 6, 2013, the Federal Financial Institutions Examination Council (FFIEC) announced that it will host two webinars with the goal of increasing cybersecurity preparedness by its member financial institutions. FFIEC's...more
On September 9, 2016 the Federal Financial Institution Examination Council (FFIEC) updated its Information Security Booklet (available here). In addition to certain editorial non-substantive changes, the modifications...more
On September 30, the CFPB released updated examination procedures for Military Lending Act (MLA) compliance. Compliance with the final rule amending the MLA, issued by the Department of Defense (DoD) in July 2015, was...more
The US Federal Financial Institutions Examination Council issued a revised Information Security booklet, which is part of the FFIEC's IT Examination Handbook. The Information Security booklet summarizes the factors necessary...more
How will examiners review the information security programs of financial institutions? Revised guidance from the Federal Financial Institutions Examination Council (FFIEC) provides help to banks by articulating the...more
The Federal Reserve Board has released a policy statement that details the framework it will follow in setting the Countercyclical Capital Buffer ("CCB") for private-sector credit exposures located in the United States. The...more
In a presidential election year, Americans are often reminded that "As Ohio goes, so goes the nation." When it comes to banks, insurers and financial institutions, it may be equally true to say "As goes New York, so goes the...more
Your first step to building a free, personalized, morning email brief covering pertinent authors and topics on JD Supra:
Sign up to create your digest using LinkedIn*
JD Supra provides users with access to its legal industry publishing services (the "Service") through its website (the "Website") as well as through other sources. Our policies with regard to data collection and use of personal information of users of the Service, regardless of the manner in which users access the Service, and visitors to the Website are set forth in this statement ("Policy"). By using the Service, you signify your acceptance of this Policy.
JD Supra collects users' names, companies, titles, e-mail address and industry. JD Supra also tracks the pages that users visit, logs IP addresses and aggregates non-personally identifiable user data and browser type. This data is gathered using cookies and other technologies.
The information and data collected is used to authenticate users and to send notifications relating to the Service, including email alerts to which users have subscribed; to manage the Service and Website, to improve the Service and to customize the user's experience. This information is also provided to the authors of the content to give them insight into their readership and help them to improve their content, so that it is most useful for our users.
JD Supra does not sell, rent or otherwise provide your details to third parties, other than to the authors of the content on JD Supra.
If you prefer not to enable cookies, you may change your browser settings to disable cookies; however, please note that rejecting cookies while visiting the Website may result in certain parts of the Website not operating correctly or as efficiently as if cookies were allowed.
Users who opt in to receive emails may choose to no longer receive e-mail updates and newsletters by selecting the "opt-out of future email" option in the email they receive from JD Supra or in their JD Supra account management screen.
JD Supra takes reasonable precautions to insure that user information is kept private. We restrict access to user information to those individuals who reasonably need access to perform their job functions, such as our third party email service, customer service personnel and technical staff. However, please note that no method of transmitting or storing data is completely secure and we cannot guarantee the security of user information. Unauthorized entry or use, hardware or software failure, and other factors may compromise the security of user information at any time.
If you have reason to believe that your interaction with us is no longer secure, you must immediately notify us of the problem by contacting us at email@example.com. In the unlikely event that we believe that the security of your user information in our possession or control may have been compromised, we may seek to notify you of that development and, if so, will endeavor to do so as promptly as practicable under the circumstances.
Except as otherwise described in this privacy statement, JD Supra will not disclose personal information to any third party unless we believe that disclosure is necessary to: (1) comply with applicable laws; (2) respond to governmental inquiries or requests; (3) comply with valid legal process; (4) protect the rights, privacy, safety or property of JD Supra, users of the Service, Website visitors or the public; (5) permit us to pursue available remedies or limit the damages that we may sustain; and (6) enforce our Terms & Conditions of Use.
In the event there is a change in the corporate structure of JD Supra such as, but not limited to, merger, consolidation, sale, liquidation or transfer of substantial assets, JD Supra may, in its sole discretion, transfer, sell or assign information collected on and through the Service to one or more affiliated or unaffiliated third parties.
This Website and the Service may contain links to other websites. The operator of such other websites may collect information about you, including through cookies or other technologies. If you are using the Service through the Website and link to another site, you will leave the Website and this Policy will not apply to your use of and activity on those other sites. We encourage you to read the legal notices posted on those sites, including their privacy policies. We shall have no responsibility or liability for your visitation to, and the data collection and use practices of, such other sites. This Policy applies solely to the information collected in connection with your use of this Website and does not apply to any practices conducted offline or in connection with any other websites.
We reserve the right to change this Policy at any time. Please refer to the date at the top of this page to determine when this Policy was last revised. Any changes to our privacy policy will become effective upon posting of the revised policy on the Website. By continuing to use the Service or Website following such changes, you will be deemed to have agreed to such changes. If you do not agree with the terms of this Policy, as it may be amended from time to time, in whole or part, please do not continue using the Service or the Website.
If you have any questions about this privacy statement, the practices of this site, your dealings with this Web site, or if you would like to change any of the information you have provided to us, please contact us at: email@example.com.
Already signed up? Log in here


text:
About this entry:
The fire-mountain in the northern parts of Mordor, in which Sauron forged the One Ring during the Second Age, and into which the same Ring fell thousands of years later to bring about the Dark Lord's downfall.
When Sauron chose the land of Mordor as his dwelling-place in the Second Age, Orodruin was the reason for his choice. He 'used the fire that welled there from the heart of the earth in his sorceries and his forging' (from Of the Rings of Power and the Third Age); the most famous result of his forging, and in fact the only one we know of for sure, was the One Ring, made in about the year II 1600. So powerful was the sorcery used in the making of the Ring that it could not be unmade, except by casting it back into the same fire that had forged it.
The first opportunity to destroy the Ring came in the last year of the Second Age, at the end of the War of the Last Alliance. Sauron was defeated in that War, and Isildur cut the Ring from his hand. Elrond and Círdan counselled him to destroy the Ring then1 (the battle had taken place at the gates of Barad-dûr, and Orodruin was therefore near at hand), but Isildur refused, and claimed the Ring for himself. So the doom of the Third Age was made.
Orodruin was far more than a natural volcano - Sauron extended his own power into it, and was able to control its fires. It seems to have lain dormant when Sauron was away from Mordor, and sprung into life when his power grew. After the Downfall of Númenor, for example, the Exiles in Middle-earth first knew that Sauron had also escaped the Downfall when they saw smoke rising from Orodruin, and an Age later at the Council of Elrond (The Lord of the Rings II 2), Boromir says 'Smoke rises once more from Orodruin that we call Mount Doom'.
It isn't clear how Elrond or Círdan could know that throwing the Ring into Orodruin would destroy it - neither of them had had any part in the making of the Rings of Power, and both had refused Sauron's lore when he offered it to them. At this time, though, both Elrond and Círdan were themselves Ring-bearers (of the Elven-rings Vilya and Narya), and perhaps these Rings gave them special insight.
Amon Amarth, Annatar, Barad-dûr, Chambers of Fire, Cracks of Doom, Days of Flight, Desolation of the Morannon, Downfall of Barad-dûr, Fiery Mountain, Fire-mountain, Great Rings, Lithlad, Lord of the Rings, Mordor, Morgul Pass, [See the full list...]Mount Doom, Mountain of Fire, Nine Walkers, Nurn, One Ring, Plateau of Gorgoroth, Quest of Mount Doom, Ring of Power, Ring-bearers, Ring-maker, Sammath Naur, Sauron, Saurons Road, Second Age, Tale of the Ring, The Fire, War of the Ring, Window of the Eye
For acknowledgements and references, see the Disclaimer & Bibliography page.
Website services kindly sponsored by Axiom Software Ltd.
Original content © copyright Mark Fisher 1997-2000, 2003. All rights reserved. For conditions of reuse, see the Site FAQ.


text:
Login Required
JPG+ Required
Collections are a JPG+ feature. You must be a JPG+ member to create new collections and to add photos to collections.
Sign up for JPG+ to start using collections now!
Photo license: © All rights reserved
2 responses
Kevin Kott (Deleted) gave props (12 May 2011):
My vote!!!
Fausto Vitali gave props (13 May 2011):
wow nice shot incredible choice of time!!!


text:
Yesterday was Bloomsday, the day that people celebrate James Joyce generally and Ulysses specifically. I had planned to let it go by unmentioned, since I don't find much in either worthy of celebration.
I was reminded this morning, however, of a famous story (possibly apocryphal) about Joyce that always interests textual scholars:
"Once or twice Joyce dictated a bit of Finnegans Wake to Samuel Beckett, though dictation did not work very well for him; in the middle of one such session there was a knock at the door which Beckett didn't hear. Joyce said, 'Come in,' and Beckett wrote it down. Afterwards he read back what he had written and Joyce said, 'What's that "Come in"?' 'Yes, you said that,' said Beckett. Joyce thought for a moment, then said 'Let it stand.'" (James Joyce, Richard Ellmann)
So much for the romantic vision of the author as an individual, a great mind working in a high and lonely place. Once again, we see that writing as collaborative. Joyce is dictating, using Beckett as a sort of on-the-fly editor. An interruption creates an error, which Joyce then accepts as an "authorized" bit of the text. In other words, for one moment at least, we have no author, just the editorial collaboration between Joyce and Beckett.
Nonetheless, we still see the existence of "Bloomsday." OK, now I know that Bloomsday isn't so much a literary event as an excuse for a pub crawl (which explains why we don't see much in the way of pilgrimages to Canterbury Cathedral on the anniversary of Chaucer's death), but some people take this very seriously. Bloomsday is an odd anachronism, a celebration of an author and his text as such, during an era in which the very idea that author or text exists is out of vogue. How can this be?
I think it is because Joyce is not so much an author as a literary celebrity. Once Ulysses had been accurately described as "unreadable," simply running one's eyes across the page became an accomplishment. Anyone who can read English can participate -- no particular knowledge of Homer, or Dublin, or literature is necessary. If you can identify the major characters (Leopold Bloom) and the major plot points (stops in Dublin), you can be part of a literary in-crowd.
Art has always had this empathetic impulse, probably essential for catharsis, for the readers/audience to identify with the characters. On the pop culture level, Nancy Drew is for girls and the Hardy Boys for boys not because the mysteries and characters are in some way different, but because the point of gender similarity makes it easier for children to place themselves in the position of the character. Art does not mirror life; life tries to mirror art. We don't just want to read narratives, we want to be in them. The popularity of "reality TV" is contingent on believing that the people on the show are "like us," and that we could, at any moment, be absorbed into the story.
Bloomsday is not about the book, I think, but more about indulging our empathetic impulse. In this case, however, the empathy moves beyond the character. We understand that in some ways Bloom is another version of Joyce himself, even to the degree that June 16th was a day of some significance to Joyce. Participants get to use the transitive property of equality (if I recall my algebra):
If A=B
and B=C,
then A=C
In this case,
If I go on Bloom's journey,
and Bloom is based on Joyce,
then I am Joyce!
Bloomsday is not really a celebration of the text -- it is an attempt to identify AS (not with) the author. Given the proverbial "unreadability" of Joyce, he makes a good candidate for this treatment, since in the backs of their minds many readers must be thinking, "Pfft! I could write this," failing to realize how difficult producing such prose would be (including knowing which accidental come ins to retain, and which to omit). Bloomsday might be a bit more sophisticated, but it is the same as putting one's own face in a cardboard cutout of a famous figure. For one day, you too can be James Joyce.
Friday, June 17, 2005
2 comments:
Subscribe to:
Post Comments (Atom)


text:
Can't find Eastern Mediterranean meeting place?
20 Feb 2009 Nikki D.Ca asked
- About
- contiki
- 0 people like this
- Answer this question 5 answers so far
20 Feb 2009 Liz Rx said
I am wondering the same thing! I posted a discussion under European Meeting and Under Cruises.
- 0 people like this
12 Oct 2009 Bradkc said
Will it be available next year (2010)??
- 0 people like this
Have an answer to this question? You need to be signed in as a Contiki.com Community member first.


text:
$.50/1 Dole Fruit Crisp - IP
I found an offer for a $.50/1 Dole Fruit Crisp Cpn on Free Coupon Alerts this morning and you can grab it also and print this great Smartsource cpn.
Click HERE to sign up today or to snag the coupon offer - click thru the link and onto Smartsource to print the cpn. Free Coupon Alerts is always updating with the latest coupons available online. They cover Printable Coupons, E-coupons and much more. Don't Miss a Coupon again with Free Coupon Alerts. This post contains affiliate links THIS POST CONTAINS AFFILIATE LINKS
The Following User Says Thank You to VACavelady For This Useful Post: | ||
The Following User Says Thank You to josieb44 For This Useful Post: | ||
Tags |
$50 or 1, crisp, dole, fruit |
Thread Tools | |
Display Modes | |


text:
Home | Register | iTrader | Members List | Calendar | Search | Today's Posts | Mark Forums Read | Store | Photo Gallery | Chat |
greatlakes4x4.com is the premier Great Lakes 4x4 Forum on the internet. Registered Users do not see the above ads.
Thread Tools | Search this Thread |
May 28th, 2006, 07:51 PM | #1 |
79 Built CJ5 For Sale or Trade!!!!
I have a 79 CJ5 that I'm looking to sell or trade. I have went through the whole jeep and have replaced almost everything except the body, which is still in reall good shape. It has an all steel body with some minor surface rust and scratches from trail riding.
It has Amc 360 rebuilt by Jasper under 1000 miles T-176 4-speed trans - rebuilt under 1000 miles Dana 300 transfer case- rebuilt under 1000 miles 33" Super Swamper TruXus STS tires under 1000 miles New 15" AR Burst wheels Full 6pt. Roll cage tieds into frame- done by KOZ New Besttop fullback seats New Besttop Top Plus there is many other little things I have replaced. Also comes with full set of Autometer white face gauges(never installed) and any other parts I have around here for the Jeep. I must sell this as all it does is take up room in my polebarn. Looking for 5500 OBO or willing to trade for???? Please let me know what you have to offer, Like I said it Must Go!!!!!! E-mail me for pics |
Thread Tools | Search this Thread |


text:
Sign in to retrieve a saved callback list.
Scores based on 172 reviews
Invoice history based on 138 reviews.
I set up J Bridges Electrical services to specialise in the domestic electrical market with the aim of providing a service of high quality workmanship, with the minimum of fuss, inconvenience and mess. And most importantly at an affordable price.
All areas of domestic electrical work are undertaken, so whether its installing a new light fitting or a complete rewire, or even just for some advice, please get in touch. I run an innovative company and continually keep up with the latest products coming onto the market so am confident I can offer you the best option.
Fully insured and registered, all work undertaken is correctly signed off.
For more information, please visit my website or Instagram page. I look forward to hearing from you.
London, Wallington, Morden, Kingston Upon Thames, Kenley, Purley, Croydon, Epsom, Ewell, Esher


text:
Friday, January 04, 2008
Color Theme for VS.NET
You can download this Visual Studio Settings here. Unzip the file and import the settings via "Tools - Import and Export Settings".
19 comments:
You know ,I have some flyff penya,and my friend also has some
flyff money,do you kouw they have the same meaning,Both of them can be called flyff gold,I just want to
buy flyff penya,because there are many
cheap penya - 5:02 AM
You know ,I have some maple mesos,and my friend also has some mesos,do you kouw they have the same meaning,Both of them can be called
maple story mesos,I just want to
maplestory mesos,because there are many
cheap mesos - 5:03 AM
You know ,I have some maple mesos,and my friend also has some mesos,do you kouw they have the same meaning,Both of them can be called
maple story mesos,I just want to
maplestory mesos,because there are many
cheap mesos - 5:04 AM
aion chinaaion china gold,
aion cn goldaion chinese gold,
aion gold chinaaion gold chinese,
china aion goldchinese aion gold,
aion china kinaaion chinese kina,
aion kina chinachina aion kina,
aion china buybuy aion china,
aion chinese server goldaion cn server gold,
aion china server goldchina aion server gold,
chinese aion server goldaion chinese server gold,
aion cn server kinaaion china server kina,
china aion server kinachinese aion server kina - 8:16 AM
- cheap habbo credits said...
I like play online game, I also gw gold and GuildWars Gold, the Guild Wars Gold is very cheap, and use the GuildWars money can buy many things, I like cheap gw gold, thanks, it is very good.
I like play online game, I also buy habbo gold and habbo credits, the habbo gold is very cheap, and use the habbo coins can buy many things, I like cheap habbo credits, thanks, it is very good. - 9:13 AM
cheap wedding gowns,
discount bridal gowns,
China wedding dresses,
discount designer wedding dresses,
China wedding online store,
plus size wedding dresses,
cheap informal wedding dresses,
junior bridesmaid dresses,
cheap bridesmaid dresses,
maternity bridesmaid dresses,
discount flower girl gowns,
cheap prom dresses,
party dresses,
evening dresses,
mother of the bride dresses,
special occasion dresses,
cheap quinceanera dresses,
hot red wedding dresses - 2:09 PM
czyszczenie sławków dąbrowa katowice chorzów. na mokro nie na sucho meblową z karcher odkurzaczem, jaworzno tanio i śmiesznie cheap. czyszczenie dywanów tanio ale także online odbywa się szybko i dokładnie wow.
Promocje związane z czyszczenie wykładzin tapicerek jest na prawdę poker tanie pranie.
Czyszczenie odbywa się u klienta. czyszczenie pranie cennik zamówienie minimum sign 40zł.
Czyszczenie na mokro jest skuteczne cennik sosnowiec czyszczenie karcher zamówienie maks 70zł.
rfs czyszczenie tapicerki pranie na mokro dla świeżości cennik galeria pranie na mokro zamówienie maks 70zł bonus. - 1:07 AM
Your article is great. Thanks for information. I'ts very useful. You have very nice blog.
Free Poker Bonus No Deposit Required - Sign up Promotions.
Free Poker bankrolls - Regards - 1:08 AM
- products said...
China Wholesalers has been described as the world's factory. buy products wholesaleThis phenomenom is typified by the rise ofbusiness. Incredible range of products available with China Wholesale "Low Price and High Quality" not only reaches directly to their target clients worldwide but also ensures that wholesale from china from China means margins you cannot find elsewhere and China Wholesale will skyroket your profits.china wholesale productsbuy china wholesalewholesale chinawholesale productsbuy products
- products said...
We offer designer , custom wedding dress, cheap cell phonescheap wedding dressesbridal gowns, wedding gowns, Flower Girl Dresses andwholesale cell phonesso on. Buy tailor-made anddesigner wedding dresses designer to be a special brides now!
- products said...
Wholesale Gemstone cheap Gemstone Jewelry from China, discount wholesale jewelry Beads, wholesale Gemstone jewelry cheapest Wholesale Naruto anime and manga cheap cosplay costumes, cheap Naruto cosplay costumes on sale, buy Naruto cosplay naruto online shop.Wholesale anime and manga wholesale crystal jewelry costumes, cheap cosplay costumes on sale, buy anime cosplay costumes online shop
- pedro velasquez said...
For a while now sportsbook I have been using a few dark color schemes for Visual Studio, mostly to just try out whether I would like it or not. Many others like Brad Wilson have also shared their adaptations and thoughts on the use of dark color themes for Visual Studio. Guru Stop and Blowmage have some additional bet nfl thoughts on dark themes that include John Lam's Vibrant Link.
I started out with some of Tomas Restrepo's color schemes and made various alterations to fit my personal preference and style. Because a dark background allows for a better distribution of color, http://www.enterbet.com and choices for color, it was worth it to see if I could be pursuaded to join the dark side. - 5:24 PM
- yiran said...
Your blog is wonderful, I like it very much, thank you!
By the way, do you like polo shirts, which are very chic, especially the polo t shirts, I love them very much. I also like playing tennis rackets, it can keep healthy, what do you like to do?
We are the outlet of polo t shirts women, polo t shirts on sale, polo t shirts for women, polo shirts on sale, these products are best-seller in our store online.besides we also sell polo shirts men, men's polo shirt, men polo shirt, mens polo shirts, mens polo shirt and cheap polo shirts, discount polo shirts, men's polo shirts, women's polo shirts We are also the outlet of cheap tennis racket, discount tennis racket and the main product is prince tennis racquet, head tennis rackets, wilson tennis racket, babolat tennis racquet. So if you love sports, you should not miss our store, we can meet what you want, and you can find many surprise in our store - 7:45 AM
- kimberly sayer said...
"Light text on dark background vs. readability" . I think that most accessibility/usability experts have no clue how hard life of a designer really is. We constantly have to battle in our mind coming up with something creative and if not we fail in our profession and will likely not convince any clients. The thing is, where do you draw the line? It is about making the right choices and that's what I did when I designed this site. I know that some things fail on this site with the color analyzer but they only contain 1 or 2 lines of text tops. My main concern was the article section and I made sure these were within the recommend guidelines. Because that's were you are likely to stay the longest.
tours of costa rica,
costa rica fishing,
saltwater fishing,
offshore fishing,
manuel antonio,
salmon fishing,
sport fishing,
fishing guide.
http://www.fishingcostaricaexperts.com - 7:22 PM
- britney sayers said...
costa rica tours, shopping online, costa rica fishing, jaco fishing charters, costa rica whale watching, costa rica dolphin watching, costa rica party boat, panama fishing, fishing costa rica los suenos, los suenos fishing charters, costa rica fishing los suenos, los suenos costa rica fishing, costa rica los suenos fishing, fishing costa rica los suenos, los suenos fishing charters, costa rica fishing los suenos, los suenos costa rica fishing charters, costa rica los suenos fishing,
http://www.kingtours.com/. - 11:06 PM
- Roxy said...
Costa Rica Manuel Antonio Rafting, Costa Rica Kayaking, Costa Rica Hiking, Costa Rica Rafting, Costa Rica Bike Tours, Costa Rica Arenal Tours, Costa Rica Guanacaste, Costa Rica Tortuguero,
Costa Rica Gay Travel, Costa Rica Gay Tours,
Costa Rica Los Suenos Vacation Rentals, Costa Rica Los Suenos Fishing, Costa Rica Los Suenos Condos, Los Suenos Costa Rica Tours,
Costa Rica Los Suenos Sportfishing, Costa Rica Los Suenos Resort,
Costa RicaWhale Watching Tours, Costa Rica Dolphing Watching Tours,Costa Rica Dominical, Costa Rica Dominical Tours, - 11:26 PM
- Costa Rica Weddings said...
Rica Fishing Packages, Costa
Rica Fishing Boats, Costa Rica Fishing,
Costa Rica Sport
Fishing Packages, Costa Rica
Fishing, Costa Rica
Fishing Packages All Inclusive, Fishing Trips Costa Rica,
Los Suenos Costa Rica Fishing,
Los Suenos Costa Rica Fishing Charters, Costa Rica Fishing Vacation Packages,
Costa Rica Fishing Packages All
Inclusive, Costa Rica Fishing
Trips, Costa Rica Fishing Trips
All Inclusive, Sport Fishing
Costa Rica, Costa Rica Sport
Fishing Charters, Sportfishing
In Costa Rica, Jaco Costa Rica
Fishing Charters, Jaco Costa
Rica Fishing Packages, Jaco
Costa Rica Sport Fishing
- Costa Rica Weddings said...
Costa Rica
Weddings, Costa Rica
Wedding Packages, Costa
Rica Weddings All Inclusive,Wedding In Jaco Costa Rica,
Costa Rica Beach Weddings,
Los Suenos Wedding,
Jaco Costa Rica Wedding,
Jaco Wedding Planner,
Costa Rica Married,
Wedding Planning Costa Rica,
Costa Rica Wedding Locations,
Costa Rica Wedding Venues,
Costa Rica Wedding Packages All
Inclusive, Costa Rica
Wedding Planner, Getting
Married Costa Rica, Costa
Rica Destination Weddings
Los Suenos Costa Rica,
Los Suenos Costa Rica Condos For Rent,
Los Suenos Costa Rica Vacation Rentals,
Los Suenos Condos For Rent,
Los Suenos Vacation Rentals,
Los Suenos Condominiums,
Los Suenos Condo Rental - 9:15 PM


text:
Lakip feeling the pressure of Georgia? Not a chance |
by Nikki Hood - Staff Writer - 2014-08-13 08:23:33.0 |
CLEMSON – Three seconds left on the game clock at Sanford Stadium late in the evening on August 30 with Clemson trailing by two, and Ammon LakipAmmon Lakip Does Lakip think about that moment? Does he feel the pressure? Yes. And no. The junior out of Johns Creek, Ga., told TigerNet following Tuesday's practice that he dreams of making the game-winning kick between the hedges, but it doesn't keep him awake at night and if people want him to stress about it, it's just not gonna happen. "Every night I go to sleep thinking about it. It's pretty exciting, I'm not going to lie," Lakip said. "I look forward to it, to be honest. I've always felt that pressure encourages my abilities. I feel calm in pressure more than anything. It's more difficult for me to show up to practice every day with a strong attitude and ready to go. Game days, I've always felt like I'm ready." Lakip has the added responsibility of trying to replace Chandler CatanzaroChandler Catanzaro "A lot of big shoes, that's all everyone wants to talk about, but personally I just do me," he said. "I've always been a little bit of a different kicker, I guess you could say. I've only kicked for five or six years now. I just like to rely on my calm attitude, my strong work ethic as far as showing up every day ready to compete no matter who I'm going against - if it's a real game or practice. I think I've matured a lot over the last three years sitting behind Catman and everyone else." Lakip played in just seven games during his first two seasons, hitting 1-of-2 field goals and 8-of-8 extra points, and he said he knows he has a lot to prove. "Personally, I haven't really proven myself on the field in college, but in high school I had two game winners my senior year," he said. "I think it comes with years of experience going against soccer players at whatever high level it was. I played on different All Star teams that traveled to Brazil, Argentina, Portugal, playing against kids who signed professional contracts probably a couple of years after we played them. I've always just felt that I've embraced high competition and people going against me that are better than me. I love proving people wrong, honestly." Early in his high school career, Lakip turned down the chance to play football so he could concentrate on soccer, but found out that football was worth a try prior to his junior season. "Freshman year, my kicking coach was my ninth grade English teacher and he was like, 'Hey, I hear you've got a strong leg. Come out and kick.' I was like, 'No, that's not for me. I want to play soccer.' I held that mentality until my junior year when they didn't have a kicker," he said. "I thought, 'You know what I'll do it, but soccer comes first.' My junior year into my senior year, I found out that my ankles weren't going to hold up playing college soccer, so I decided that it was about time I just focused on football. It was the way I had to go. When one door closes, another one opens so I just took it seriously from then on and it got me here." Lakip said that there isn't a day that goes by that he doesn't miss playing soccer, but his experience on the soccer field led him to trust his instincts and his feet – an essential element to his kicking technique. "I miss it every day, but I get out here and I kick balls, so it's close enough to it," he said. "You just have to come before a game and watch me kick the football. I just have this feel for any ball - I trust my feet more than my hands, honestly. A lot of kickers are real anal about their technique. I'm anal, but more or less I just trust my instincts to kick a ball where I want it to go. I think that separates me from a lot of kickers who grew up using fundamentals." Being a kicker can sometimes make you an outsider in the locker room, but Lakip said that over time he has adjusted to being part of the football team and he even uses some of the moves he learned on the soccer field to impress his teammates. "I've always felt like I was a pretty personable guy, but I definitely had to learn a mindset especially in the weight room, coming here with all of these guys who want to throw up heavy weights and stuff," he said. "It's taken me a while to adapt, but I think I've grown to embrace my niche as "The Kicker" that you see him do some stuff with his feet and you're just like, 'Ok.' Especially with the game FIFA - everyone sees it and is like, 'Wow, you used to do that stuff.' I've grown to become great friends with all of these football players. I know a bunch of guys over there playing soccer. I played against them growing up. I just try to embrace myself as, "The Kicker" because we're different. Any kicker would say that we're different. We're specialist and we have to be ready when called upon in a game."
RS Jr. Kicker
#36 5-10, 200
Alpharetta, GA
View Full Profile lines up for the game-winning field goal that would start off Clemson's season on a high note.
Punter / Kicker (2009 - 2013)
#39 6-2, 195
Greenville, SC
View Full Profile, a reliable weapon for the Tigers over the last four years. Lakip said that while he understands who he is trying to replace, he can't be Catanzaro – he can only be Ammon Lakip.
Send Feedback to Nikki Hood: Email | Comment |


text:
A van at one of the entrances to the Philippine House of Representatives in suburban Quezon City, north of Manila late Tuesday Nov. 13, 2007 as police and forensic officers work at the scene.
PERFECTO CAMERO / AP Enlarge
MANILA, Philippines - A bomb exploded at an entrance of the Philippine House of Representatives late Tuesday, killing a congressman and a driver.
Police and soldiers in the capital went on high alert after the explosion, which injured nine people.
The blast happened as the House ended its session shortly after 8 p.m., officials said.
"At this point in time I'm saying it's a bomb, but I can't say what type of bomb," National police chief Avelino Razon said.
A fireman hoses down a vehicle at one of the entrances to the Philippine House of Representatives in suburban Quezon City, north of Manila, after an explosion late Tuesday Nov. 13, 2007.
PERFECTO CAMERO / AP Enlarge
Political tensions are high in the country. President Gloria Macapagal Arroyo is facing a third impeachment complaint in as many years. Muslim militants also have bombed targets in the capital in the past.
Rep. Wahab Akbar's chief of staff said the congressman died of wounds in a hospital, and Manila's police chief Geary Barias said a lawmaker's driver also was killed.
Read more in later editions of The Blade and toledoblade.com


text:
All of your business and communication software apps are important in the project management. But what are the unique ways that these tools are used for project management, and what are the project management-specific tools? This page includes some of the interesting PM software things we thought you might be interested in.
• creating workflows across multiple platforms and applications • orchestrating ITIL process inputs and outputs • dynamic resource allocation, load balancing • linking IT services to business requirements, business impact analysis • dynamic thresholding, heuristic monitoring
The standard MS Office Suite is probably the most commonly used PM software. Although not a new trend, this favored position is likely to change over time.
It can easily be augued that MS Outlook is the most commonly used project management software in existence today. Why? Because 90% of a project managers job is communication. And 2nd only to verbal communication, e-mail communication is the mode.
Visit this link to see a group of project management planning tools are done in Excel.
Often a tool for making Gantt charts and network diagrams, Visio is a wonderful flow-charting application. Visio takes a little time to learn, and it's primary purpose was not project management. Yet it is an absolutely great tool - and just as powerful as many of the software applications that were created specifically for project management.
Here is a direct quote from one of our students on Oct. 12, 2009:
"I've been obsessively laying out my project plan for next year and updating my network diagram. I even created a Gantt chart. This is incredibly helpful! I saw that I need to crunch my network diagram in order to hit specific dates when I can and can't do things. I really love Visio, although I am still a novice. One thing that really came to light for me this weekend was that all of these tools really help ensure buy in and success. If I post the Gantt chart for my team to see for next year, it will help build teamwork and buy in to ensure we are all focused on getting this project done. I know communication and teamwork are only part of PM but for me this is so critical. It was like a light bulb went off! I am very excited to share this with my supervisors as we begin working on some larger projects in our library this fall. I anticipate I will be working more with Visio."
- Certificate in Project Management student with Successful Projects
The common choice for developing project plans, use cases, requirements gathering, and other written plans.
The common choice for presenting information at stakeholder and team meetings.
Successful Projects doesn't use this software as of yet but many of our clients do. And they seem pretty happy with it. The product web site is at http://www.attask.com/.
Information coming soon.
MS Project has been, and remains to be, the most well-used project management software - although probably not the most popular. It's popular because it is intuitive for Microsoft users. It integrates well with Outlook and the other Office Suite products. And it is a commonly used tool, so it is easy to find other users and good local training help and support.
One of the problems to watch out for is that when resource leveling, the software will reassign work and change the schedule. And there is no undo for this act.
The current individual license price is about $450. The version on the market right now is Project 2010, but Project 2007 is still very commonly used.
For MS Project training, we recommend Cindy Prindle, who instructs through Hawkins, Ash, and Baptie in La Crosse, Wisconsin.
Use of Skype, AdobeConnect Now, and other video conferencing tools is becoming important.
This is a mindmapping software that helps to brainstorm and visualize ideas quickly. It currently sells for $389.
It is supposed to integrate with timelines, Gantt charts, and MS Office software integration for help with project planning.
The site is here.


text:
overoptimistically is a valid word in this word list. For a definition, see the external dictionary links below.
The word "overoptimistically" uses 18 letters: A C E I I I L L M O O P R S T T V Y.
No direct anagrams for overoptimistically found in this word list.
Adding one letter to overoptimistically does not form any other word in this word list.
Words within overoptimistically not shown as it has more than seven letters.Try a search for overoptimistically in these online resources (some words may not be found):
Wiktionary -
OneLook Dictionaries -
Merriam-Webster -
Google Search
Each search will normally open in a new window.
List all words starting with overoptimistically, words containing overoptimistically or words ending with overoptimistically
All words formed from overoptimistically by changing one letter
Other words with the same letter pairs: ov ve er ro op pt ti im mi is st ti ic ca al ll ly
Browse words starting with overoptimistically by next letter
Previous word in list: overoptimistic
Next word in list: overoptimists
Some random words: aphaereses ikat cafe arles eikon pe blab
This is not a dictionary, it's a word game wordfinder.
- Help and FAQ - Examples - Home
Privacy and Cookies Policy - Share - © Copyright 2004-2017 Morewords.com - 27.127mS


text:
Your favorite Apple, iPhone, iPad, iOS, Jailbreak, and Cydia site.
Thread: Time Magazine: iPhone 5 is 2012 'Gadget of The Year'
- Join Date
- Aug 2009
- Location
- Valparaiso, Indiana
- Posts
- 4,376
- Thanks
- 17
- Thanked 1,491 Times in 577 Posts
Time Magazine: iPhone 5 is 2012 'Gadget of The Year'
Time Magazine has bestowed a lofty honor upon Apple's iPhone 5. Time has selected Apple's latest model as the 2012 "Gadget of the Year."
The report from Time author Harry McCracken describes the iPhone 5 as "one of the most artfully polished gadgets anyones ever built, with a taller screen than previous iPhones built into a thinner, lighter case."
And while Android devices still lead the iPhone in terms of units sold around the world for 2012, Time gives Apple the nod for being the smartphone leader in terms of general device and OS quality.
There are lots of nifty smartphones out there, including the iPhones impressive archrival, Samsungs Galaxy S III. But when it comes to melding hardware, software and services so tightly that the seams fade away, Apple still has no peer.
- Apple iPhone 5
- Nintendo Wii U
- Sony Cyber-shot RX100
- Raspberry Pi Model B
- Lytro
- Apple 15-inch MacBook Pro with Retina display
- Microsoft Surface with Windows RT
- Samsung Galaxy Note II
- Nest
- Simple.TV
Source: Time
- Join Date
- Mar 2011
- Location
- Virginia
- Posts
- 753
- Thanks
- 33
- Thanked 146 Times in 93 Posts
Where's the galaxy s3?
Seems like a pretty ****** list if you ask me. Good products but the order of em is off
The Following User Says Thank You to unixman84 For This Useful Post:
KraXik (12-12-2012)
- Join Date
- Feb 2009
- Location
- Texas
- Posts
- 1,121
- Thanks
- 4
- Thanked 95 Times in 67 Posts
Sure it is.
And I'm sure this has absolutely nothing to do with the clever little iPad Mini ad that was run on Time Magazine's cover.
Moving on......
I'm sorry to break it to you guys but the Galaxy S3 is better than the iphone 5 in nearly every way. The iPhone 5 is much better in terms of build quality but that's about it. Even that's a double edged sword. Sure, the aluminum looks and feels great but it shows scratches, dings and nicks like crazy. I don't worry about dropping my GS3 cause I know it won't shatter like my iPhone 4 did or get a big gash like an iPhone 5 would. Android as an os is just so much more flexible and usable. I was a iOS devotee for three years but I bought an iPhone 5 and the sense of deja vu was just immediately a bummer. Plus all the apps that don't fill the screen, the garbage lightning connector and the locked down os was enough to make me sell it on eBay for $800. I bought a GS3 for $450 and have loved it. I still like iOS for a tablet, Android can't touch it when it comes to tablet optimized apps, but as a phone os Android has blown past iOS. I hope Jony Ive can make some meaningful changes because I still love my Mac and iPad and would love to have all my devices in perfect harmony again.
Last edited by sofakingstewpit; 12-11-2012 at 09:22 PM.
- Join Date
- Nov 2007
- Location
- Bermuda
- Posts
- 48,833
- Thanks
- 8,084
- Thanked 17,601 Times in 8,002 Posts
Does seem odd that the S3 isn't on there. Although it seems they didn't really put competitors in each ranking. It's like they did the best of each category (smartphones, tablets, laptops etc.) and then just ranked the categories.
- Join Date
- Apr 2008
- Location
- Posts
- 658
- Thanks
- 74
- Thanked 173 Times in 111 Posts
I had the GS3 for 2 weeks. Literally dropped it 2ft off of my lap and the screen shattered. Sold on eBay for $300 as is. I bought the iPhone 5 and have dropped mine many times with no screen damage what so ever. Few scratches here and there. You are full of shyt brah. Go watch drop comparison video's the GS3 is garbage. Plastic junk, Samsung is ripping you off. I've owned every iPhone and 3 Android phones. As far as a phone iOS wins hands down. Why wouldn't you want a simple phone that works perfect out of the box without having to root it and all that stuff? Mobile iOS is perfect in every sense for a phone. iMessage is amazing with the ability to send full quality pics/videos. Group chat works flawlessly. It's horrible on Android. It's so bad on Android you need a 3rd party app lol which is the case for many basic features on Android. You'll have to trust a 3rd party app with your messaging and even keyboard.
Camera/video - iPhone 5 > GS3 Android is grainy and worse in photo/video quality
Build quality - iPhone 5 > GS3 Brb plastic phone
Battery - iPhone 5 > GS3 No comparison here lets be real dude
Phone performance iPhone 5 > GS3 Android still lags! Idc what blueberry pancakes apple pie OS you run, it will lag
Browser speed iPhone 5 > GS3 LTE or WiFi the iPhone is faster. And don't mention flash brah that's a thing of the past. No longer mobile supported
Apps/games iPhone 5 > GS3 Everything runs smoother/faster on iOS
Updating software iPhone 5 > GS3 Enjoy waiting months for your carrier broski
Phone backup iPhone 5 > GS3 No comparisons here. iTunes or iCloud win by a long shot
Video chat iPhone 5 > GS3 FaceTime is the best
Charging time iPhone 5 > GS3 iPhone 5 charges so fast and will last a long time
Here's the big one brah, WARRANTY. Apple Care pwns Samsung's warranty. Walk into the Apple Store and 99% of the time you'll walk out with a new iPhone, refirb or not. Apple Care is one of the best warranties around.
I've been learning about the galaxy s3 a lot and although I've only owned an iphone my girlfriends sister does own a Htc and my mom owns an android. As far as being reliable. Usable and friendly. Apple takes the cake. The galaxy s3 is an OK phone but it is pretty cluttered and disorganized with all the third party apps that you have to download. As far as practical goes iphone wins. Samsung just kinda goes overboard with all the features and apps they shove in. I've finally understood how and why apple takes its time In slowly releasing features and gives you good reasons why. Nothing against android as I might own a future s4 one day but right now it's a good cluttered phone
- Join Date
- Aug 2010
- Location
- Wales, UK.
- Posts
- 113
- Thanks
- 0
- Thanked 6 Times in 5 Posts
I'm surprised the raspberry pi isn't #1 on the list.
Wow brah, broski, bromo, whatever you want to be called, you got me there. Your super intelligent response to my points speaks for itself. Have fun setting up Sparrow, a clearly superior email client, as your default client. Oh wait, you can't. Well at least you can make Chrome your default...oh nevermind, can't do that either. iMessage is a freaking joke. I've had iMessages disappear into thin air never to be seen again. I've had them show up an hour later than I've sent them. Please stop with that nonsense. The camera is a little better on the iPhone 5 but please don't act like its a night and day difference. Also, get a big boy camera and don't rely on a phone camera for important pictures anyway. The battery is bigger and lasts longer on the GS3 2100mah>1434mah, brah. I know you know how those greater than signs work so hopefully you can decipher that. Not to mention you can swap batteries on the GS3, but you can do that right...oh wait...Build quality belongs to the iPhone 5 like I said but have fun with your scratched aluminum. There is zero lag with Jelly Bean on my GS3. Just because you weren't smart enough to figure out how to get your phone running well doesn't mean it doesn't work for others. As far as LTE speeds go, I've gotten 50 down on my GS3 versus 48 down on my iPhone 5. What does that even matter though. They're are both blazing fast and I would argue that you couldn't tell the difference between 20 down and 50 down on a phone anyway. Who cares about flash?!?! It's not even supported anymore anyway. Sure, iOS gets offical updates faster but they break the jailbreaks and they sometimes suck. Are you running iOS 6 brah? Hopefully not. I downgraded my iPhone 4 back to iOS 5.1.1 because iOS 6 was trash. Backups are better on Android as well. Have you used Titanium Backup? I'm guessing you haven't because you would know it's better than having to go to your computer and load up the lovely program known as iTunes. Titanium Backups can be restored from your micro sd card. Speaking of expandable storage...Facetime is no better or worse than any myriad of video chat applications. And my GS3 may take slightly longer to charge but that's because it has a bigger battery. Well bro, I hoped you enjoyed getting worked over. Next time you try to argue it's best to know what you are talking about. Maybe your awesome Maps app can help lead you to a place where you can actually convince someone that the iPhone 5 is better. Nah...Also, maybe use less greater than/less than symbols, you just look like a fool. Galaxy S3 > iPhone 5.
Last edited by sofakingstewpit; 12-12-2012 at 09:31 AM.
- Join Date
- Nov 2007
- Location
- Bermuda
- Posts
- 48,833
- Thanks
- 8,084
- Thanked 17,601 Times in 8,002 Posts
So tired of the endless "iPhone 5 is better, no S3 is better, iOS is better, no Android is better". It's all the same. They more or less both do the same thing, it is just a matter of personal preference. I have owned a S3. Didn't like it. Right now I have a iPhone 5 and a Nexus 4 and love both. I prefer my iPhone 5 as my main phone, but not by much, and that is mostly because of me having been in the apple world for so long (iTunes, music, games etc.). iOS is smoother and easier to get around but has become a little stale. Android is not as polished and can be a bit cumbersome but sure is fun to play around with all the stuff you can do with it.
So in summary: iPhone 5 = Samsung S3
You can still use flash on the S3 on jelly bean, it's just not updated or in the play store any more but can easily be found and side loaded. Works fine on 4.1.2
Haha, funny how most if not all of the apps you mentioned are 3rd party apps. The camera is not slightly better, and yeah I'll carry around a 1500 hundred dollar camera around my neck 24/7 in case I happen to see something I like. The GPS in my area at least is way better on the iPhone than on my cousins gs3, most of the time his runs are way off because he kept getting disconnected, his gryroscope isn't even half as good also. The s3 can be a better phone but is nowhere near as good when you compare the 2 out of the box. And yes it does have a bigger battery but it lasts less so don't be showing that off, it may last more on stand by but not if you use your phone like a normal person would. I live in the US near the border and maps work great here and Mexico. Just cause you read about ppl having problems, there are thousands not having them. The s3 is a great phone but don't try and pretend its better than the iPhone 5.
Yeah, they're third party apps. But that's the point. If a third party makes a better app that can be your default app. Don't act like stock apps are better just because they are stock. I used an iPhone 5 for two weeks before I sold it so I'm not just speaking from what I've read. I live in a major metropolitan area and the maps app lead me in a circle of u-turns on more than one occasion. I put the iPhone's battery through its paces while I had it and it doesn't last as long as my GS3 does, especially since the Jelly Bean update. I've had both and the iPhone camera is only slightly better. It's not like anybody is gonna blow up a phone camera picture to an 8x10 anyway and they both produce fine results when viewed on their respective screens. I really do feel like the iPhone 5 was a fine phone, the best Apple has ever made even, it's just the exact same phone as the 4 and the 4s. The only difference there is that you can't jailbreak the 5. We all know how much we love stock iOS. I mean that's why we come to this site, right? So we can read all about the cool tweaks for stock iOS...
Last edited by sofakingstewpit; 12-12-2012 at 12:54 PM.
Again, the iPhone is a great phone. I'm just saying that it's great for people who don't care to get the most from a mobile device. It's too limited. Android isn't perfect, not by a long shot, but it's much better than iOS if you care to learn all of it's tricks.
Last edited by sofakingstewpit; 12-12-2012 at 03:29 PM.
- Join Date
- Oct 2007
- Location
- London uk
- Posts
- 890
- Thanks
- 92
- Thanked 73 Times in 59 Posts
I think you're the one talking S! Here's my 2pence worth....Had a GS3 for about 5 months and it was mostly awesome, the only thing I hated was the bezel got random cracks in it after the first month. I was so disappointed so I sold mine on and thought id give the i5 a go, BIG MISTAKE!!!! This bezel makes the GS3 seem like titanium. Even with a case my black is still wearing out and if it wasnt for the fact I paid £340 (without contract) I would have sold this too and went back to Gs3. i5 is same old same old. So I call bs on this list and say GS3 should be at top. I have to agree that apples service is good tho and since 2007 they have never turned me down and sorted my problems over all my idevices where samsung would not even acknowledge the cracks etc.Last edited by se23; 12-13-2012 at 01:31 PM.


text:
Monday, November 7, 2011
Crazy Cooking Challenge - Mashed Potatoes with Caramelized Onions and Goat Cheese
21 comments:
These sound amazingly delicious and I love all the flavors.
If you haven't already I'd love for you to check out my Crazy Cooking Challenge entry Aloo Tikki
Cook Lisa CookI'm not a big fan of goat cheese but otherwise these look so deliious. I just love caramelized onions!
Jen #14
Jenny's CookbookThis was one of the recipes I was thinking about making for the challenge... glad I didn't lol! But yours looks and sounds delish. I am obsessed with caramelized onions!! I'm #71, check me out if you like :)
http://steaknpotatoeskindagurl.blogspot.com/2011/11/crazy-cooking-challenge-and-loaded.htmlYummmy.. This looks amazing.. What a lovely recipe.. I have to try it sometime.. I would LOVE if you could please link it to my thanksgiving {three weeks of thanks} party pweease - Via http://theartsygirlconnection.blogspot.com/2011/11/thankful-for-food.html Wishing you an awesome start to your week.. ~M


text:
Virulence, bacterocin genes and antibacterial susceptibility in Enterococcus faecalis strains isolated from water wells for human consumption. | |
Jump to Full Text | |
MedLine Citation:
PMID: 23449751 Owner: NLM Status: Publisher |
The objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of E. faecalis isolated from water wells for human consumption. The virulence and bacteriocin genes of 78 E. faecalis were amplified by PCR and visualized in agarose gels. The antimicrobial susceptibility was determined through diffusion agar tests and the MIC through microdilution. It was observed that the major percentage of virulence genes in the E. faecalis strains corresponds to aggA (93.5%). The bacteriocin gene entA (64.1%) is the most frequently detected. The studied strains exhibited different virulence and bacteriocin genes, and an important antibacterial resistance. The most common resistant phenotype (n = 14) corresponds to tetracycline and chloramphenicol and the less frequent (n = 2) to ciprofloxacin and moxifloxacin. Eight different genetic profiles were observed for virulence y bacteriocin genes. It was determined a statistical association between the bacterial resistance and some of the genetic profiles detected. |
Carlos Padilla; Olga Lobos |
Publication Detail:
Type: JOURNAL ARTICLE Date: 2013-2-9 |
Journal Detail:
Title: SpringerPlus Volume: 2 ISSN: 2193-1801 ISO Abbreviation: Springerplus Publication Date: 2013 Dec |
Date Detail:
Created Date: 2013-3-1 Completed Date: - Revised Date: - |
Medline Journal Info:
Nlm Unique ID: 101597967 Medline TA: Springerplus Country: - |
Other Details:
Languages: ENG Pagination: 43 Citation Subset: - |
Departamento de Microbiología, Universidad de Talca, Talca Camino Lircay s/n, Talca, Chile. |
Export Citation:
APA/MLA Format Download EndNote Download BibTex |
MeSH Terms | |
Full Text | |
Journal Information Journal ID (nlm-ta): Springerplus Journal ID (iso-abbrev): Springerplus ISSN: 2193-1801 Publisher: Springer International Publishing AG, Cham |
Article Information Download PDF © Padilla and Lobos; licensee Springer. 2013 Received Day: 22 Month: 11 Year: 2012 Accepted Day: 31 Month: 1 Year: 2013 Electronic publication date: Day: 9 Month: 2 Year: 2013 pmc-release publication date: Day: 9 Month: 2 Year: 2013 collection publication date: Month: 12 Year: 2013 Volume: 2 Issue: 1 E-location ID: 43 PubMed Id: 23449751 ID: 3579423 Publisher Id: 86 DOI: 10.1186/2193-1801-2-43 |
Virulence, bacterocin genes and antibacterial susceptibility in Enterococcus faecalis strains isolated from water wells for human consumption | |
Carlos PadillaAff1_86 |
Address: email@example.com |
Olga LobosAff1_86 |
Address: firstname.lastname@example.com |
Departamento de Microbiología, Universidad de Talca, Talca Camino Lircay s/n, Talca, Chile |
Representants of the genus Enterococcus are part of the normal human gut microbiota, some animals, and birds. The Enterococcus out of the organism can survive in different temperature ranges, salinity, pH and resist some detergents as dodecyl-sodium sulfate and bile (Shepard and Gilmore 2000). Phylogenetically the enterococci are divided into 7 clusters resulting finally in 33 species (Naser et al.2005; Köhler 2007). In human infections, E. faecalis and E. faecium are the most prevailing species (>90%). The first is the most commonly isolated, but recently E. faecium has exhibited an important increase as infectious agent (Treitman et al.2005). The enterococcal infection includes surgical wounds, endocarditis, hepatobiliary sepsis, urinary tract infections, bacteremias and neonatal sepsis among the most important (Poh et al.2006). The majority of the enterococcal infections are endogeneous, but the crossed infection occurs mainly in hospitalized patients (Cookson et al. 2006). Enterococcus in USA is the fourth most common cause of nosocomial infection and the third of bacteremia (Biendenbach et al.2004). In Europe these infections are less and represent a 7.2% of the total Martínez-Odrizola et al. (2007).
Enterococcal strains resistant to different antibiotics are a great public health problem, especially in isolated strains from hospital-acquired infections (Hidron et al. 2008). Several genetic studies have described the complex functions related to horizontal gene transfer in enterococcal strains, which partly explains their high antimicrobial resistance (Arthur et al.1993; Clewell et al.1995). Vancomycin was the antimicrobial agent most active against this bacterium, however, today in different parts of the world, isolated enterococcal strains are resistant to vancomycin (Ridwan et al. 2002; Chang et al.2010).
Various factors determine the E. faecalis virulence, outstanding among those on the cellular surface, as the aggregation substance (Agg), the extracellular surface protein (Esp) and some that are excreted out of the bacterial cell as cytolysin and hyaluronidase, among others (Fisher and Phillips 2009; Semedo et al.2003; Koch et al. 2004). Additionally, the Enterococcus spp. synthesizes heterogeneous antibacterial peptides or bacteriocins, also called enterocins (Giraffa 1995). These products may give these bacteria an additional ecological tool to persist in environments colonized by competing microorganisms in order to remove them and take over the ecological niche (Padilla et al. 2006).
In recent years, E. faecalis has a greater ability to cause infections on different human epithelia (Fisher and Phillips 2009). Thus, more studies are needed to provide new knowledge about this bacterial specie
Currently, there is considerable information regarding the antimicrobial susceptibility, presence of virulence genes and bacteriocins in E. faecalis strains isolated from clinical specimens and food. However, it would also be important to study if strains that come from water wells possess the properties mentioned and if it is possible to transmit these characteristics to the intestinal microbiota of people who drink this water.
Is important to remark that in some rural areas of central Chile, where there is no drinking water, people use well water for drinking and for various domestic purposes.
For a better understanding of the biology of E. faecalis, the objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of E. faecalis isolated from water wells for human consumption.
Were studied 128 water wells. In 78 of them were isolated 78 E. faecalis strains in the Maule Region in central Chile during the year 2010. From each well were taken 20 ml of water with a sterile bottle at a deep of about 5 cm. The samples were taken within 2 h to the microbiology laboratory of the University of Talca where they were processed immediately. The water samples were diluted 1:9 in sterile distilled water and 50 μl of this dilution was plated on Bile-esculin agar (Merck, Darmstadt, Germany) and incubated at 37°C for 24 h. The identification of the 78 bacterial isolates was carried out through conventional biochemical tests (Facklam and Collins 1989).
The antibacterial susceptibility test was performed through the diffusion in agar test (Bauer et al. 1996). The antimicrobials analyzed were: ampicillin, tetracycline, ciprofloxacin, moxifloxacin, gentamicin, vancomycin, chloramphenicol y erythromycin (VALTEK, Santiago, Chile). For determination of MIC, the antimicrobial were prepared in accordance with the manufacturers' instructions (Sigma, St Louis, MO, USA) and in accordance with the recommended by EUCAST (2012). All the resolutions of the MICs were performed in duplicate. E. faecalis strain ATCC 29212 was included as control in assays with or without antimicrobials.
The total DNA of E. faecalis strains was used in the PCR reactions to detect the presence of virulence genes as cytolysin (cylA), aggregation substance (aggA), antigen A (efaA), stimulating of pheromones expression (eep), gelatinase (gelE) and surface protein (esp). Additionally were amplified the genes for enterocins AS-48 (as-48), bacteriocin 31 (bac31), enterocins A, B and P (entA, entB, entP), enterocin L50 A/B (entL50A/B), enterolysin A (enlA) and enterocin 1071 (ent1071). Each PCR reaction contained 10 mM Tris–HCl buffer pH 8.3, 1.5 mM MgCl2, 50 mM KCl, 200 μM of each deoxinucleotide (Omega, BioTek, Doraville, GA), 1 μM of oligonucleotide, 2U of Taq DNA polymerase and 15 ng of DNA template (Table 1).
The reactions conditions for each gene were the following:
For the amplification of the virulence genes cylA, aggA, efaA, eep, gelE and esp, the samples with the DNA templates were denatured at 94°C by 5 m. Subsequently, 30 cycles were performed: 45 s at 94°C, 1 m at 57°C and 1 m at 72°C for the gene cylA; 30 cycles of 45 s at 94°C, 1 m at 52°C and 1 m at 72°C for the aggA and efaA genes; 30 cycles of 45 s a 94°C, 1 m at 58°C and 1 m at 72°C for the eep gene; 30 cycles of 30 s at 94°C, 30 s at 56°C and 30 s 72°C for the gelE gene; 30 cycles of 45 s at 94°C, 1 m at 62°C and 1 m at 72°C for the esp gene. The amplification of all the virulence genes was concluded with a final extension of 72°C by 3 m.
The PCR were performed in a thermal DNA Engine (Bio-Rad, Hercules, CA). The negative controls (without DNA template) were included in each reaction set and all performed double. The amplification products were analyzed through electrophoresis in agarose gels at 1.5% stained with ethidium bromide and visualized under ultraviolet light. The size of each amplified product was confirmed by comparison with the molecular size marker 1Kb-Plus DNA ladder (New England Biolabs).
The correlation between the presence of virulence and bacteriocin genes with the antimicrobial susceptibility of the E. faecalis strains, was determined using the Test of χ2 of Test Exact of Fisher, as it corresponded. A p < 0.05 value was considered statistically significant to indicate the association between both variables. The statistical package used was SPSS v.19.0.
From 128 water wells were isoalted 78 E. faecalis strains and were identified by conventional biochemical test.
In the Table 2 it was observed that the entA gene (64.1%) is the most frequently detected followed by the entL50A/B (37.5%) and the enlA (26.9%) gene respectively. The ent P (8.9%) gene was the less frequent. The gene as-48 was not detected. In the Table 3, it is observed that 11 of the total of the studied strains exhibit 6 virulence genes and 2 bacteriocin genes. The main genetic profile was observed in 18 E. faecalis strains, in which 4 different virulence genes and 2 bacteriocinogenic genes were detected. The less common profile corresponded to 5 strains that carried 3 virulence genes and 2 bacteriocinogenic genes. Eight genetic profiles were determined that include virulence and bacteriocin genes (A, B, C, D, E, F, G y H profiles).
The Table 4 shows the resistance phenotypes (MICs) of the 78 E. faecalis strains. It is observed that 3 phenotypes exhibit resistance to 4 antibiotics simultaneously and 3 phenotypes show resistance to only one. In the same table, it is observed that the most common resistance phenotype (n = 14) corresponds to a tetracycline and chloramphenicol and the less frequent (n = 2) to ciprofloxacin and moxifloxacin. It was not observed resistance to vancomycin in any of the strains evaluated.
In accordance to statistical analysis it was observed a significant association between the resistance to tetracycline and chloramphenicol with the genetic profiles A, B, C, D and F and of sensitivity in presence of the genetic profiles E and H. It was observed that ampicillin resistance is associated to the genetic profiles A, B, C y D and of sensitivity with E and F (p < 0.0001). The genetic profiles associated with the resistance to gentamicin were A, B, D and F and of sensitivity C and E (p < 0.0001). Erythromicin, ciprofloxacin, moxifloxacin and vancomycin did not exhibit a significant statistically susceptibility association with the genetic profiles detected.
In the Table 5, it is observed that the E. faecalis strains that exhibit a greater number of virulence and bacteriocin genes were more resistant to the antimicrobial. The greatest number of resistant strains was associated with tetracycline followed by chloramphenicol, ampicillin and gentamicin respectively. The lowest resistance of the strains was observed for moxifloxacin and ciprofloxacin.
Enterococcus faecalis is a very important bacterial specie as a pathogen acquired in hospitals of USA and Europe, especially in intensive care units, where are one of the main causes of bacteremia (Rodríguez-Baño et al.2005). Although there are various surveys addressed to know the causes because these microorganisms induce human infections, diverse aspects of its biology have not been revealed (Fisher and Phillips 2009).
The results demonstrated that the majority of the bacterial strains had genes that codify for different virulence factors. The results of this study are coherent with the described in other studies (Koch et al.2004; Dupont et al. 2008). The enterocin genes analyzed in this work represent the most frequently described in this bacterial specie (Franz et al.2007). The bateriocinogenic activity of E. faecalis is widely recognized and its bacteriocins have been object of interesting biotechnological studies associated to the biopreservation of foods (Khan et al. 2010; Poeta et al.2007). The bacteriocinogenic capacity of these microorganisms grants an ecological superiority with respect to those strains that do not produce these antibacterial peptides. This property also grants survival advantages to the bacteriocinogenic strains, because these products are able to eliminate to potential competing microorganisms and start subsequently the colonization phase. It was very interesting to observe that all the studied strains exhibited two different types of genes for bacteriocins, which added to its respective virulence genes grant to these strains a greater pathogenic potentiality. Thus, bacteriocinogenic strains have an additional ecological tool to persist in environments colonized by competing microorganisms. Probably once introduced in the organism with the water these strains can more easily colonize; on the contrary they do not have an ecological benefit in aquatic environment where virulence factors and bacteriocins are diluted.
It was shown in this finding that most of the strains exhibit resistance to two or more antimicrobials. Specifically 25 strains present resistance to four different types of the antimicrobials used. This important resistance associated to high MICs confirms the recognized antimicrobial property of E. faecalis which is maintained in the studied strains. Also, in accordance to statistical analysis it is possible to argue that genetic profiles A, B, and D of E. faecalis are more frequently associated to resistance against some antimicrobials and inversely, the profiles C and E are associated with sensitivity.
The evolution and ecology of E. faecalis, is a subject of great importance and that must be constantly monitored to have new information to allow to know the biology of this bacterial specie. The results of this study demonstrate that intensive use of water wells for human consumption includes poor sanitary practices. This is the cause of bacterial contamination of the wells with human microbiota. This could explain the presence of E. faecalis in these wells. Although, generally contamination of the wells is due to environmental contamination of groundwater by fecal pollution, it is possible to consider that E. faecalis strains analyzed are circulating among the wells and the people who use this water resource. The observed properties in the studied bacterial strains are similar to those found in strains isolated from clinical samples. On the other hand, these strains are a serious problem for people who drink this water contaminated with E. faecalis, considering that these bacteria could transfer genetic information from antimicrobial resistance to their intestinal microbiota (De Niederhäusern et al. 2011).
Competing interest
The authors declare that they have no competing interest.
Authors' contributions
CP: carried out part of the bacterial identification; carried out part of the molecular genetic studies; to made part of the writing and design of the manuscript; carried out the statistical analysis OL: carried out part of the bacterial identification; carried out part of the molecular genetic studies; to made part of the writing and design of the manuscript; carried out the statistical analysis The authors declare: All authors read and approved the final manuscript.
Arthur M,Molina C,Depardieu F,Courvalin P. Characterization of TN1546, a Tn3 – related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147J BacteriolYear: 19931751171278380148 | |
Bauer A,Kirby M,Sherris J,Turck M. Antibiotic susceptibility testing by a standardized single disk methodAmer J Clin PatholYear: 1996454934965325707 | |
Biendenbach DJ,Moet GJ,Jones RN. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY antimicrobial surveillance programDiag Micr Infec DisYear: 200450596910.1016/j.diagmicrobio.2004.05.003 | |
Bittencourt de Marques E,Suzart S. Occurrence of virulence- associated genes in clinical Enterococcus faecalis strains isolated in Londrina, BrazilJ Med MicrobiolYear: 2004531069107310.1099/jmm.0.45654-015496382 | |
Chang CM,Wang LR,Lee HC,Lee NY,Wu CJ,Ko WC. Characterization of vancomycin-resistant enterococci from hospitalized patients at a tertiary center over a seven-year periodJ Hosp InfectYear: 20107437738410.1016/j.jhin.2009.10.02520170984 | |
Clewell DB,Flannagan SE,Jaworski DD. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposonsTrends MicrobiolYear: 1995322923610.1016/S0966-842X(00)88930-17648031 | |
Cookson BD,Macrae MB,Barret SP,Brown DJ,Chadwick C,French GL,Hateley P,Hosein IK. Guidelines for the control of glycopeptide-resistant enterococci in hospitalsJ Hosp InfectYear: 20066262110.1016/j.jhin.2005.02.01616310890 | |
De Niederhäusern S,Bondi M,Messi P,Iseppi R,Sabia C,Manicardi G,Anacarso I. Vancomycin-resistance transferability from VanA enterococci to Staphylococcus aureusCurr MicrobiolYear: 2011621363136710.1007/s00284-011-9868-621234755 | |
Du Toit M,Franz CM,Dicks LM,Holzapfel WH. Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faecesJ Appl MicrobiolYear: 20008848249410.1046/j.1365-2672.2000.00986.x10747229 | |
Dupont H,Vael C,Muller-Serieys C,Chosidow D,Marmuse J,Andremont A,Goossens H,Desmonts J. Prospective evaluation of virulence factors of enterococci isolated from patients with peritonitis. Impact on outcomeDiag Microb Infect DisYear: 20086024725310.1016/j.diagmicrobio.2007.10.006 | |
EUCAST disk diffusion method for antimicrobial susceptibility testingYear: 2012 | |
Facklam R,Collins M. Identification of Enterococcus species isolated from human infections by a conventional test schemeJ Clin MicrobiolYear: 1989277317342656745 | |
Fisher K,Phillips C. The ecology, epidemiology and virulence of EnterococcusMicrobiologyYear: 20091551749175710.1099/mic.0.026385-019383684 | |
Franz CM,Van Belkum MJ,Holzapfel WH,Abriouel H,Gonçalvez A. Diversity of enterococcal bacteriocins and their grouping in a new classification schemeFEMS Microbiol RevYear: 20073129331010.1111/j.1574-6976.2007.00064.x17298586 | |
Giraffa G. Enterococcal bacteriocins: their potential use as anti-Listeria factors in dairy technologyFood MicrobiolYear: 199512551556 | |
Hidron AI,Edwards JR,Patel J,Hora TC,Sievert DM,Pollock DA,Fridkin SA. NHSN annual update: antimicrobial-resistant pathogenes associated with heathcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control Prevention, 2006–2007Infect Control Hosp EpidemiolYear: 200829996101110.1086/59186118947320 | |
Khan H,Flint S,Yu PL. Enterocins in food preservationInt J Food MicrobiolYear: 201014111010.1016/j.ijfoodmicro.2010.03.00520399522 | |
Koch S,Hufnagel M,Theilackers C,Huebner J. Enterococcal infections host response therapeutic and prophylactic possibilitiesVaccineYear: 20042282283010.1016/j.vaccine.2003.11.02715040934 | |
Köhler W. The present state of species within the genera Streptococcus and EnterococcusIJMMYear: 200729713315017400023 | |
Martínez-Odrizola P,Muñoz-Sánchez P,Gutierrez-Macías A,Arriola-Martínez P,Montero-Aparicio E,Ezpeleta-Baquedano C. Análisis de 182 episodios de bacteremia por enterococo: estudio de la epidemiología, microbiología y evolución clínicaEnferm Infecc Microbiol ClinYear: 20072550350710.1157/1310998617915108 | |
Naser S,Thompson FL,Hoste B,Gevers D,Vandmeulebroecke K,Cleenwerck I,Thompson C,Vancanneyt M. Phylogeny and identification of enterococci by atpA genee sequence analysysJ Clin MicrobiolYear: 2005432224223010.1128/JCM.43.5.2224-2230.200515872246 | |
Padilla C,Lobos O,Brevis P,Abaca P,Hubert E. Plasmid-mediated bacteriocin production by Shigella flexneri isolated from dysenteriae diarrhea and their transformation into Escherichia coliLett Appl MicrobiolYear: 20064230030310.1111/j.1472-765X.2005.01829.x16478521 | |
Poeta P,Costa D,Rojo-Bezares B,Zarazaga M,Klibi N,Rodriguez J,Torres C. Detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animalsMicrobiol ResYear: 200716225726310.1016/j.micres.2006.06.00316872815 | |
Poh CH,Oh HM,Tan AL. Epidemiology and clinical outcome of enterococcal bacteraemia in an acute care hospitalJ InfectYear: 20065238338610.1016/j.jinf.2005.07.01116203039 | |
Ridwan B,Mascini E,Van Der Reijden N,Verhoef J,Bonten M. What action should be taken to prevent spread of vancomycin resistant enterococci in European hospitals?BMJYear: 200232466666810.1136/bmj.324.7338.66611895829 | |
Rodríguez-Baño J,Ramírez E,Muniain M,Santos J,Joyanes P,González F,García-Sánchez M,Martínez-Martínes L. Colonization by high-level aminoglycoside-resistant enterococci in intensive care unit patients. Epidemiology and clinical relevanceJ Hosp InfectYear: 20056035335910.1016/j.jhin.2004.12.02415893852 | |
Sabia C,Niederhaüsen S,Guerrieri E,Anacarso I,Manicardi G,Bondi M. Detection of bacteriocin production and virulence traits in vancomicyn-resistant enterococci of different sourcesJ Appl MicrobiolYear: 200810497097910.1111/j.1365-2672.2007.03612.x18005029 | |
Semedo T,Santos MA,Lopes MF,Marquez JJ,Crespo MT,Tenreiro R. Virulence factors in food, clinical and reference enterococci: a common trait in the genes?Syst Appl MicrobialYear: 200326132210.1078/072320203322337263 | |
Shankar V,Baghdayan AS,Huycke MM,Lindahl G,Gilmore MS. Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface proteinInfect ImmunYear: 1999671932009864215 | |
Shepard BD,Gilmore MS. Differential expression of virulence related genes in Enterocococcus faecalis in response to biological cues in serum and urineInfect ImmunYear: 2000704344435210.1128/IAI.70.8.4344-4352.200212117944 | |
Treitman AN,Yarnols PR,Warren J,Noskin GA. Emerging incidence of Enterococcus faecium among hospital isolates (1993 to 2002)J Clin MicrobiolYear: 20054346246310.1128/JCM.43.1.462-463.200515635016 | |
Eaton TJ,Gasson MJ. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolatesAppl Environ MicrobiolYear: 2001671628163510.1128/AEM.67.4.1628-1635.200111282615 |
Oligonucleotide sequences used in this study and their products
Virulence genes | Oligonucleotide sequences | Product size (bp) | Reference |
cylA | for5′-TGGATGATAGTGATAGGAAGT-3′ | 517 | Eaton and Gasson 2001 |
rev5′-TCTACAGTAAATCTTTCGTCA-3′ | |||
aggA | for5′-AAGAAAAAGTAGACCAAC-3′ | 1553 | Eaton and Gasson 2001 |
rev5′-AACGGCAAGACAAGTAAATA-3′ | |||
efaA | for5′-GACAGACCCTCACGAATA-3′ | 705 | Eaton and Gasson 2001 |
rev5′-AGTTCATCATGCTGCTGTAGTA-3′ | |||
eep | for5′-GAGCGGGTATTTTAGTTCGT-3′ | 937 | Bittencourt de Marques and Suzart 2004 |
rev5′-TACTCCAGCATTGGATGCT-3′ | |||
enlA | for5′-TTCTTCTTATTCTGTCAACGCAGC-3′ | 960 | Bittencourt de Marques and Suzart 2004 |
rev5′-GACTGTGAAATACCTATTTGCAAGC-3′ | |||
esp | for5′-TTGCTAATGCTAGTCCACGACC-3′ | 933 | Shankar et al. 1999 |
rev5′-GCGTCAACACTTGCATTGCCGAA-3′ | |||
Bacteriocin genes | |||
as-48 | for5′-GAGGAGGAGTATCATGGTTAAAG-3′ | 340 | Sabia et al. 2008 |
rev5′-CATATTGTTAAATTACCAAGCAATAA-3′ | |||
bac31 | for5′-GAAAAAGAAATTAGTTATTTGTG-3′ | 202 | Sabia et al. 2008 |
rev5′-CTATCTAGGAGCCCAAGG-3 | |||
entA | for5′-ATGAAACATTTAAAAATTTTGTCTA-3′ | 130 | Sabia et al. 2008 |
rev5′-TTAGCACTTCCCTGGAATTG-3′ | |||
entB | for5′-GAAAATGATCACAGAATGCCTA-3′ | 160 | Du Toit et al. 2000 |
rev5′-GTTGCATTTAGAGTATACATTTG-3′ | |||
entP | for5′-GTTAGTTTGTGACAAATTTTGG-3′ | 120 | Sabia et al. 2008 |
rev5′-GGTGTGGAAAAGCCGTTTC-3′ | |||
entL50 A/B | for5′-TGGGAGAATCGCAAAATTAG-3′ | 96 | Du Toit et al. 2000 |
rev5′-ATTGCCCATCCTTCTCCAAT-3′ | |||
ent1071 | for5′-ATATTTAGGGGGACCGATAA-3′ | 405 | Sabia et al. 2008 |
rev5′-TATACATTCTTCCACTTATTTTT-3′ |
Virulence and bacteriocin genes in 78Enterococcus faecalisstrains isolated from water wells for human consumption
Virulence genes | Nº | % |
aggA | 73 | 93.5 |
cylA | 60 | 76.9 |
efaA | 37 | 47.4 |
eep | 31 | 39.7 |
esp | 55 | 70.5 |
gelE | 24 | 30.7 |
Bacteriocin genes | ||
entB | 13 | 16.6 |
ent 1071 | 8 | 10.2 |
ent P | 7 | 8.9 |
entL50A/B | 29 | 37.5 |
enlA | 21 | 26.9 |
entA | 50 | 64.1 |
bac31 | 11 | 14.1 |
as-48 | 0 | 0 |
Virulence and bacteriocin genetic profiles in 78E. faecalisstrains isolated from water wells for human consumption
Genetic profiles | Strains | ||
Virulence genes | Bacteriocins | nº | % |
(A) * efaA, aggA, esp, gelE, cylA, eep | entA, bac31 | 11 | 14.1 |
(B) efaA, aggA, esp, gelE, cylA | entA, entB | 8 | 10.2 |
(C) efaA, aggA, esp, gelE | entA, entL50A/B | 18 | 23.0 |
(D) aggA, esp, cylA, eep | enlA, entP | 7 | 8.9 |
(E) aggA, esp, cylA | entL50A/B, enlA | 11 | 14.1 |
(F) cylA, gelE, eep | entA, entB | 5 | 6.4 |
(G) cylA, eep, aggA | entA, ent 1071 | 8 | 10.4 |
(H) cylA, aggA, gelE | entA, enlA | 10 | 12.8 |
Total | 78 | 100 |
*: Name of genetic profile.
MICs of several antimicrobials against different resistance phenotypes ofE. faecalisisolated from wells of water for human consumption
Median MIC (range) (mg/L) | |||||||||
Resistance phenotype | Numbers of strains | T | A | V | C | M | Ch | G | E |
T A Ch G | 8 | 64 (16–256) | 16 (4–128) | 64 (32–256) | 16 (8–128) | ||||
G A Ch E | 10 | 16 (8–64) | 64 (32–128) | 32 (16–64) | 16 (8–64) | ||||
T A Ch E | 7 | 32 (8–128) | 8 (8–64) | 16 (8–128) | 16 (8–64) | ||||
T Ch | 14 | 64 (16–256) | 64 (32–256) | ||||||
Ch | 5 | 64 (32–256) | |||||||
Total | 78 |
T: tetracycline; A: ampicillin; V: vancomycin ; C: ciprofloxacin; M: moxifloxacin; Ch: chloramphenicol; G: gentamicin; E: erythromicin.
Relation of virulence and bacteriocin genetic profiles and their respective antimicrobial resistance inE. faecalisstrains isolated from water wells for human consumption
Virulence and bacteriocin | Nº of resistant strains and antimicrobials genetic profiles | ||||||||
T | A | V | C | M | Ch | G | E | ||
efaA, aggA, esp, gelE, cylA, eep | entA, bac31 | 11 | 9 | 0 | 2 | 1 | 11 | 8 | 3 |
efaA, aggA, esp, gelE, cylA | entA, entB | 6 | 8 | 0 | 1 | 0 | 8 | 8 | 4 |
efaA, aggA, esp, gelE | entA, entL50A/B | 12 | 11 | 0 | 1 | 1 | 11 | 3 | 3 |
aggA, esp, cylA, eep | enlA, entP | 5 | 4 | 0 | 0 | 0 | 5 | 7 | 2 |
aggA, esp, cylA | entL50A/B, enlA | 3 | 3 | 0 | 0 | 0 | 0 | 4 | 1 |
cylA, gelE, eep | entA, entB | 3 | 1 | 0 | 0 | 0 | 3 | 4 | 1 |
cylA, eep, aggA | entA, ent 1071 | 4 | 2 | 0 | 0 | 0 | 4 | 2 | 0 |
cylA, aggA, gelE | entA, enlA | 5 | 2 | 0 | 0 | 0 | 2 | 3 | 3 |
Total | 49 | 40 | 0 | 4 | 2 | 44 | 39 | 17 |
T: tetracycline; A: ampicillin; V: vancomycin ; C: ciprofloxacin; M: moxifloxacin; Ch: chloramphenicol; G: gentamicin; E: erythromicin.
Article Categories:
Keywords: Keywords E. faecalis, Wells, Water, Bacteriocin, Virulence, Antimicrobials. |
Previous Document: Development of an online education program for midwives in australia to improve perinatal oral healt...
Next Document: A novel technique for attaining maxillomandibular fixation in the edentulous mandible fracture.


text:
Cisco offers channel a carrot
Cisco's channel partners have paid scant attention to its SAN specialization program, prompting the company to offer them a rebate if they get involved.
Cisco Systems Inc. has taken to dangling a carrot in front of its channel partners in the form of a rebate if they...
Continue Reading This Article
Enjoy this article as well as all of our content, including E-Guides, news, tips and more.
By submitting your personal information, you agree that TechTarget and its partners may contact you regarding relevant content, products and special offers.
You also agree that your personal information may be transferred and processed in the United States, and that you have read and agree to the Terms of Use and the Privacy Policy.
join its SAN specialization program and -- more importantly -- sell its storage switches.
Since it launched the SAN specialization program last June, Cisco claims to have signed up 35 resellers. However, according to Cyndi Privett, analyst with View Point Research, very few have actually taken the course and become certified to sell the gear. "When I last checked in November, only one reseller had qualified," she said.
In an effort to convert those resellers that have signed up and to encourage others to join, Cisco has introduced the Clear Advantage program, which translates sales of its MDS 9000 storage switches into points, with each point being worth $1. Points are derived by taking 5% of the average OSM (original storage manufacturer) (OSM) transfer costs.
Unlike other Cisco products, resellers have to first purchase the MDS 9000 switches from Cisco's OSM partners that include, EMC Corp., IBM, Hitachi Data Systems, Hewlett-Packard Co. and Xiotech Corp. This is known as the transfer cost and is inhibitive to resellers as they must shell out for the gear before making any sale themselves. Cisco set up its storage channel this way because it had to have the major storage players involved in selling its technology first to establish a presence in the market.
The outcome, according to Privett, meant that resellers had "several reasons not to join the SAN program and very few reasons to do it." And yet she thinks Cisco should clean up in this market. "Cisco has captured good market share in a year without really putting a lot of muscle into it … This is a first step towards really turning up the heat." She added that Cisco has some of the world's best resellers selling its products.
There are approximately 150 key storage resellers out there that Cisco is courting. Chris Lusk, director of open systems and storage at Sirius Computer Solutions Inc., a San Antonio reseller, is one of them. Lusk said the program compares favorably to McData Corp.'s rebate program, although McData's program, which pays a set fee per transaction, is easier to use.
He added that Cisco's growth targets are pretty high, 20% per quarter, but partners that miss this goal can make up the numbers the following quarter or at the end of the year.
Overall, Cisco's storage sales are improving. Last quarter, the company reported 40% sequential growth and a 70% year-over-year increase in MDS switch sales. Still, that 40% equates to approximately $56 million in revenue, which is small fry for a company that turned a profit of $1.4 billion last quarter. It's also a long way off Cisco's publicly stated goal of reaching $1 billion or more in revenue related to storage applications.
Clear Advantage program requirement
In order to qualify for the program, channel partners must be Cisco-registered partners. The rebate is paid based on meeting the entry-level criteria, milestone criteria and achieving the revenue goals during the entire period in which the partner participates in the Clear Advantage program, including submitting a business plan and becoming a Cisco SAN specialized partner.
To participate in the program's first two quarters, partners must apply by March 21, of this year. The Clear Advantage program is currently available in the American, European, Middle Eastern and African sales regions. However, each region's program initiatives and prerequisites are customized based on local market needs. Click here for more information about the Cisco Clear Advantage program.
Click here for more of today's news.Dig Deeper on SAN switch
Find more PRO+ content and other member only offers, here.


text:
Collections are a JPG+ feature. You must be a JPG+ member to create new collections and to add photos to collections.
Sign up for JPG+ to start using collections now!
Photo license: © All rights reserved
Quebec, Canada
9 responses
Michele Wambaugh (Deleted) said (13 Aug 2012):
Looks so inviting.
Susan Littlefield gave props (13 Aug 2012):
I'm always amazed at the trees in the forest. Some grow in the most unique way, and some right out of rock. A terrific capture!
Fred Moskey said (13 Aug 2012):
A cool shot!
Dhanny P (Deleted) gave props (13 Aug 2012):
I love forested photos.. The light and shadows can produce different photos on any given day.. this is beautiful
Davide Simone (Deleted) gave props (13 Aug 2012):
Elfriede, Wonderful !!!! love the colors
Lynn E. Harvey gave props (14 Aug 2012):
I just love this. Nature at it's best..beautiful trees!
Carlo Pagan gave props (18 Aug 2012):
Oregon Curly gave props (18 Aug 2012):
Wonderful composition, the 'bent' tree looks like it is just trying to reach and hold up the leaning tree beside it.
Judy and Wayne Wanamaker gave props (20 Aug 2012):
Reaching for the light! An amazing capture! The forest is so beautiful.