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HERV-H RNA is abundant in human embryonic stem cells and a precise marker for pluripotency
- Federico A Santoni1, 2Email author,
- Jessica Guerra2 and
- Jeremy Luban2, 3Email author
© Santoni et al.; licensee BioMed Central Ltd. 2012
Received: 5 December 2012
Accepted: 16 December 2012
Published: 20 December 2012
Certain post-translational modifications to histones, including H3K4me3, as well as binding sites for the transcription factor STAT1, predict the site of integration of exogenous gamma-retroviruses with great accuracy and cell-type specificity. Statistical methods that were used to identify chromatin features that predict exogenous gamma-retrovirus integration site selection were exploited here to determine whether cell type-specific chromatin markers are enriched in the vicinity of endogenous retroviruses (ERVs).
Among retro-elements in the human genome, the gamma-retrovirus HERV-H was highly associated with H3K4me3, though this association was only observed in embryonic stem (ES) cells (p < 10-300) and, to a lesser extent, in induced pluripotent stem (iPS) cells. No significant association was observed in nearly 40 differentiated cell types, nor was any association observed with other retro-elements. Similar strong association was observed between HERV-H and the binding sites within ES cells for the pluripotency transcription factors NANOG, OCT4, and SOX2. NANOG binding sites were located within the HERV-H 5′LTR itself. OCT4 and SOX2 binding sites were within 1 kB and 2 kB of the 5′LTR, respectively. In keeping with these observations, HERV-H RNA constituted 2% of all poly A RNA in ES cells. As ES cells progressed down a differentiation pathway, the levels of HERV-H RNA decreased progressively. RNA-Seq datasets showed HERV-H transcripts to be over 5 kB in length and to have the structure 5′LTR-gag-pro-3′LTR, with no evidence of splicing and no intact open reading frames.
The developmental regulation of HERV-H expression, the association of HERV-H with binding sites for pluripotency transcription factors, and the extremely high levels of HERV-H RNA in human ES cells suggest that HERV-H contributes to pluripotency in human cells. Proximity of HERV-H to binding sites for pluripotency transcription factors within ES cells might be due to retention of the same chromatin features that determined the site of integration of the ancestral, exogenous, gamma-retrovirus that gave rise to HERV-H in the distant past. Retention of these markers, or, alternatively, recruitment of them to the site of the established provirus, may have acted post-integration to fix the provirus within the germ-line of the host species. Either way, HERV-H RNA provides a specific marker for pluripotency in human cells.
HERV-H Endogenous retrovirus Pluripotency Long non-coding RNA Embryonic stem cell Induced pluripotent stem cellBackground
Vertebrate genomes contain retroviral sequences that are believed to be remnants of exogenous retroviral infection from the distant past [1]. The genesis of these endogenous retroviruses (ERVs) necessitates establishment of provirus by the ancestral, exogenous retrovirus within host germ cells, such that these elements are maintained as heritable genetic elements in the host species. Human endogenous retroviruses (HERVs) are not uncommon, and account for at least 8% of the total human DNA. By comparison with DNA sequences from exogenous retrovirus families, three main classes of HERVs, gamma, beta, and delta have been identified [2]. Phylogenetic analyses identified HERV-K, a betaretrovirus, and HERV-H, a gammaretrovirus, as the most recent entries into the genomes of primates, 10 and 25 million years ago, respectively [3, 4].
Though some HERVs are transcriptionally active, most retroviral sequences in the human genome are corrupted by mutations or successive insertion of transposable elements; most HERVs lack intact open reading frames for viral protein production and no autonomously replicating HERV has been identified. As a result, HERVs are generally considered non-functional, junk DNA. In some cases, though, HERVs make significant - even essential - contributions to the normal physiology of the host species [5–8]. HERVs have also been implicated in the development of pathological conditions [9–14]. Interestingly, HERVs can be activated by exogenous retrovirus infection [15] and, in reciprocal fashion, the immune response to these elements can influence the outcome of infection by pathogenic, exogenous retroviruses such as HIV-1 [16, 17].
Exogenous retroviruses integrate into locations throughout the host chromosomal DNA in a quasi-random fashion (reviewed extensively in [18]). Previously, we developed statistical tools to identify association between the sites of provirus establishment and chromosome marks – as determined by chromatin immuno-precipitation with massively parallel DNA sequencing (ChIPSeq) [18, 19]. From this analysis we identified chromatin modifications (H3K4me3, H3K4me1, and H3K9ac) and binding sites for transcription factors (STAT1) that predict the site of integration for gamma-retroviruses with great accuracy in a cell-type specific manner [18, 19]. Precise markers such as these were not identified for other classes of retrovirus such as the lentivirus HIV-1 [18]. Here, the same statistical methods were exploited to determine whether any ERVs retain association with cell type-specific chromatin features that might have determined the site of integration in the distant past, or that served to fix the provirus in the genetic patrimony of the host species.
Search for chromatin features near the site of ERVs
Data used for analysis of endogenous retroviruses
Data type GEO accession number | Factor | Cell type or tissue | Reference |
Human | |||
ChIPSeq, GSE16256 | H3K4me3, H3K27me3 | H1, H9, I3, iPS, IMR90 | [20] |
ChIPSeq, GSE22499 | H3K4me3 | BG01/03, WIBR1/2/7, hiPSA6/C1, Fibroblast | [21] |
ChIPSeq, GSE15353 | H3K4me3 | HeLa w/o IFN | [22] |
ChIPSeq, GSE19465 | H3K4me3 | hiPS-11a/18c/15b/20b, duodenum mucosa, BM-MSC,smooth muscle, adult liver, fetal lung, fetal brain, CD34, CD3, CD15, CD19, Pancreatic Islets | [23] |
ChIPSeq, GSE20650 | NANOG,OCT4, KLF4 | H1 | [24] |
ChIPSeq, GSE18292 | SOX2 | H1 | [25] |
RNASeq, GSE23316 | H1, HeLa, K562 | UCSC ENCODE Project | |
RNASeq, GSE20301 | H1, differentiated H1 | [26] | |
Mouse | |||
ChIPSeq, GSE22075 | Mouse H3K4me3 | ES, LSK cells | [27] |
ChIPSeq, GSE12241 | Mouse H3K4me3 | ES, MEF | [28] |
HERV-H expression in ES cells
It is also important to consider that expression of endogenous retro-elements might be influenced by local effects from adjacent transcriptional units. The difference between the expression level of the repetitive elements and the expression level of the surrounding sequences was assessed (see Methods for details). By this measure, HERV-H exhibited transcriptional specificity comparable to that of conventional genes, in terms of specific/unspecific transcription ratio (0.85 vs. 0.86), while LINEs and SINEs had a transcription ratio of 0.06 and 0.03, respectively (Figure 7).
HERV-H expression over the course of human ES cell differentiation
HERV-H and pluripotency transcription factors
Association of LINE elements with NANOG in human ES cells
This study discovered a strong association between the genomic location of HERV-H proviruses and H3K4me3-modified histones, that is exclusive to human ES and some iPS cells. Consistent with H3K4me3 serving as a marker for active transcription, HERV-H expression was high in these pluripotent stem cells. Moreover, the pluripotency transcription factors NANOG, OCT4 and SOX2 bound to the LTRs of transcriptionally active HERV-H proviruses, or within 2 kB of them. NANOG, OCT4 and SOX2 frequently co-occupy the promoters of target genes, many of which are transcription factors that regulate development such as homeodomain proteins [41].
These observations strongly support the hypothesis that HERV-H transcripts play a role in human pluripotency and that this role is finely regulated by three of the most important transcription factors in ES cells. In addition to the binding of NANOG, OCT4, and SOX2 to the HERV-H promoter, HERV-H RNA decreased as ES cells differentiated, in a manner that was proportional to the expression of NANOG and OCT4. Conversely, HERV-H RNA was undetectable in primary fibroblasts but increased enormously after forced re-programming to generate pluripotent stem cells (unpublished data provided by Audrey Letourneau and Stylianos Antonarakis). HERV-H, then, can be exploited as a reliable marker of ES cell pluripotency, as well as an indicator of the degree of "stemness" of iPS cells as they are generated from fibroblasts.
HERV-H transcripts are 5 to 6 kB in length and lack open reading frames. We can only speculate about the function of these lncRNAs. They might, for example, serve to soak-up miRNAs that promote differentiation, as has been shown with linc-MD1 in muscle differentiation [42] or the PTENP1 pseudogene in the regulation of PTEN and growth suppression [43]. They might bind to chromatin and act as a scaffold for the local recruitment of pluripotency transcription factors, similar to other lncRNAs like HOTAIR for histone modification complexes [44] and Xist in the context of X-chromosome inactivation [44–46]. Alternatively, HERV-H might counteract retrovirus spread by interfering with packaging of retroviral genomic RNA [47, 48] or by soaking up miRNAs that are required for retrovirus transduction.
The study here failed to identify chromatin markers that associate with endogenous retro-elements in mice. This was somewhat surprising given the many endogenous retro-elements in this species, including endogenous gamma-retroviruses, some of which are intact and functional [8]. It was also surprising because exogenous gamma-retroviruses have the same integration site preferences in mouse cells as they have in human cells [18]. MLV integration sites are associated with the H3K4me3 profile in mouse embryonic fibroblasts (F score = 0.83; p < 10-100). Similar results with murine hematopoietic stem cells (F score of 0.81; p < 10-100) indicate that, as in human cells, the association strength is cell-type dependent.
One possible explanation for the failure to identify chromatin markers associated with endogenous murine retroviruses is species-specific differences in the recruitment of the transcriptional silencing machinery. In murine ES cells, for example, a sequence-specific DNA-binding protein, ZNF809 [49], recruits TRIM28 and other components of the cellular machinery that silence MLV [50]. ZNF809 has no orthologue in humans; perhaps ZNF809 arose as a result of selective pressure exerted by murine specific gamma-retroviruses during evolution.
Previous work demonstrated that when exogenous retroviruses integrate they home to sites of H3K4me3 [18]. Similarly, the association of endogenous gamma-retrovirus HERV-H with H3K4me3 suggests that when human and simian germ cells were bombarded with the HERV-H ancestor 15 to 30 millions years ago, these ancient retroviruses integrated in proximity to H3K4me3-marked chromatin. These proviruses might then have retained these cell-type specific marks as they became fixed in the primate genome. Alternatively, unmethylated HERV-H LTRs might have recruited chromatin remodeling factors and induced H3K4me3 modification of the viral promoter after integration had occurred.
Analysis of the DNA surrounding HERV-H proviruses failed to clarify which of these two scenarios is more likely. Additionally, search for epigenetic markers like H3K4me3 in syntenic regions in the mouse genome was attempted to determine if these chromatin marks are conserved across the species and predate the entry of HERV-H into the primate genome. DNA surrounding HERV-H proviruses in the human genome was aligned to the mouse genome (using the tool LiftOver, http://genome.ucsc.edu/cgi-bin/hgLiftOver) after excision of all repetitive elements (performed with RepeatMasker, http://www.repeatmasker.org/). Nothing informative was found by measuring the association of mouse H3K4me3 with these syntenic regions and comparing these values with those obtained using control loci.
P300 and H3K27ac bind intra-species conserved regions and co-localize in embryonic-specific enhancers [34, 51]. These markers are also associated with HERV-H in human ES cells, lying within 4 kB of 80% of HERV-H proviruses (F score 0.95; p < 10-300). It seems unlikely that an exogenous retrovirus would be capable of recruiting these factors by exploiting random conserved regions around its integration site. This suggests that a pre-existing layer of epigenetic markers favored integration of HERV-H into particular host loci and that these features are still preserved millions of years later.
Among retroelements in the human genome, the endogenous gammaretrovirus HERV-H is extraordinary for its high level expression in embryonic stem cells, in which it makes up 2% of all polyadenylated RNA. The human genome has ~1,000 copies of HERV-H, and the majority of the HERV-H RNA is encoded by a subset of 50 of these. HERV-H expression decreases as ES cells lose pluripotency, to the point where its expression is undetectable in fibroblasts. Consistent with this expression pattern, HERV-H is also expressed to high level in many iPS cells, though expression in some iPS cells is more modest; this heterogeneity may reflect reported differences in the epigenetic profile of many iPS cells, when compared with ES cells [29]. This suggests, then, that HERV-H RNA offers a relatively stringent marker for human pluripotency that would be worth monitoring during the generation of new iPS lines. The HERV-H RNAs in ES cells average about 5 kB in length and encode no protein. It, therefore, seems likely that HERV-H RNA contributes to pluripotency by acting as a chromatin-associated structural element or by acting as a microRNA decoy.
Statistical analysis of association between chromatin markers and retro-elements
where is the central locus of the provirus.
As a control dataset, we randomly selected 100000 genomic locations. Association strength was measured with the statistical method based on the F score, as previously described in [18].
Formally the F β -score is defined as the β-weighted harmonic mean of Precision(P) and Sensitivity(R): .
Here β = 0.5 to give more weight to Precision than to Sensitivity. This balances type I and type II errors by adjusting for the high rate of False Positives inherent to the examination of large datasets for genome-wide binding sites according to statistical significance (F score based statistics and comparison with other measures have been extensively discussed in [18]).
Markers with F scores ranging between 0.5 and 1 were considered to be associated with endogenous integration sites.
RNASeq data analysis
HERV-H is present in more than 1000 "imperfect" copies in the human genome and its transcripts share a number of short conserved regions (each around 100 bp). Therefore, deep sequencing of those transcripts yields reads (25-75 bp long sequences) which perfectly align to several genomic loci. Indeed, multireads mapping is still a challenging process [52]. The strategy adopted here was to perform the alignment of uniquely mapped single- and paired-end reads and to reassign the multiple-mapped reads in function of the expression level of the surrounding (context) region [26]. HERV-H expression was evaluated in term of "Reads Per Kilobase per Million mapped reads (RPKM)" with the standard formula [53] where N r is the number of reads mapping onto the r transcript, L r is the length (in kB) of the r esim transcript and N T is the total number of reads K = 106.
Alignments of RNASeq generated reads have been performed with a two-step procedure. First we used Bowtie[54] on raw data, admitting up to two mismatches for each alignment, and then we discriminated between unique mapping reads and multireads, 80% and 20% of the total number of reads respectively. As expected, many multireads matched with repeated elements. Ignoring them would have resulted in a potential underestimation of the expression of endogenous retroviruses and, in general, of all repetitive elements. Therefore, we adopted a probabilistic assignment based on the amount of reads that univocally map onto the surrounding regions (context), as described in more detail in the next paragraph. This evaluation is also useful to establish if a repeated element is expressly transcribed or if it is part of another structure (i.e. a gene).
Probabilistic alignment of multiple mapping reads
Eventually, the set of multireads mapping to the same M regions is then partitioned to {S1,…,SM} accordingly to P D (s i ).
The first axiom of high throughput sequencing asserts that the number of reads aligned to a specific genomic region is proportional to how much RNA has been generated by this region within the cell. At this point it is worth observing that repeated element (RE) sequences might be present in the RNA just because they are part of longer mRNAs.
where , as before, is the function giving the number of reads assigned to the region s.
Human endogenous retrovirus
Embryonic stem
- iPS:
Induced pluripotent stem
Long terminal repeat
- lncRNA:
Long non-coding RNA.
The authors wish to thank Audrey Letourneau and Stylianos Antonarakis for providing unpublished RNA-seq data. This work was supported by NIDA/NIH/USA Grant DP1DA034990 and Swiss National Science Foundation grant 3100A0-128655 to J.L.
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- Pepke S, Wold B, Mortazavi A: Computation for ChIP-seq and RNA-seq studies. Nat Methods. 2009, 6: S22-S32. 10.1038/nmeth.1371.PubMed CentralView ArticlePubMedGoogle Scholar
- Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B: Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008, 5: 621-628. 10.1038/nmeth.1226.View ArticlePubMedGoogle Scholar
- Langmead B, Trapnell C, Pop M, Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009, 10: R25-10.1186/gb-2009-10-3-r25.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
By Diana
- gardening for biodiversity
in Cape Town, South Africa
Two great heaps of sandy soil at the bottom of the garden
from the Ungardener digging the pond, and me each time I dig a hole for the
next plant. After Camps Bay days I am loath to garden on even a tiny slope. I
dug my heels in for concrete retaining blocks and raised beds. Two layers of neatly
lined up fluted columns. Ivy pelargoniums will cascade down from the blocks,
and 3 little trees wait patiently. For End of month view.

subset: commoncrawl


text:
Raps Continuing to Pursue Kyle Lowry
The Raptors will continue to pursue Houston PG Kyle Lowry, regardless of whether or not they're able to land Steve Nash.
https://twitter.com/ESPNSteinLine/st...21497138561024 |
Re: Raps Continuing to Pursue Kyle Lowry
This is pure BS.
Raps trying to save face for not acquiring Lowry when they had the chance. Houston probably won't trade him now that Dragic asking price is so high. BC is going down in flames. |
Re: Raps Continuing to Pursue Kyle Lowry
Can't see the Rockets bringing back a very unhappy and disgruntled Lowry. Also, the Rockets appear to be making an offer to Jeremy Lin. |
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Hawking: 'There is no heaven'
Mon May 16, 2011Will you try a frozen IHOP item?
Mon May 9, 2011Man sentenced in death of mother who had maggots
Tue Apr 26, 2011'Birther' claims force GOP leaders to take a stand
Fri Apr 22, 2011Should death row inmates be allowed to donate their organs?
Wed Apr 20, 2011US scrapping 'cascading' terror alerts
Wed Apr 20, 2011Headscarves slam brakes on women's careers
Wed Apr 20, 2011Farewell, debit reward cards
Wed Apr 6, 2011What's your take on hell?
Thu Mar 24, 2011Taco Bell takes to airwaves to defend their beef
Mon Feb 28, 2011


text:
First time here? Check out the
Ask a Question
How to download images in bluestacks?
Answer possibly found in:
How to save my pictures from BlueStacks WhatsApp to my PC?
Since you cannot export the pictures right out of the
, I recommend installing Dropbox. You can upload your files to Dropbox, then browse to website and
the pictures. This is the...
Thanks for trying
and sharing your issue. Please find below some steps that will help you in uploading pics on instagram:- You can access the data from windows using Instagram in...
Its very easy to copy the data from bluestacks to your PC. Just
Astro File Manager and install it using
. Its android browser. After installation, open it. You will get a folder...
all non sense raveena ,u just open C drive then documents and settings then all users then application data then bluestacks then u will definitely get the required folder"user data" 100 per...
this application complete running , thanks to answer informer..
In your Bluestacks, 1. Launch file manager. 2. Copy the "whats app" folder. 3. Go to "bstfolder" and to "Bstsharedfolder" 4. Click on paste. After all that, you go to the following link...
in your blue stacks
file manger then copy your
from that file manager following path --- file manger/whatsapp/media/whatsappimages AND then paste it in same file manager but follow...
there is no folder like User data in bluestacks
try this.... instal astro file mgr, open it...open pictures,then there is a view button below,select directory options ,select show hidden files...apply..after that u can see a thumbnails folder...
go to C:\ProgramData\BlueStacks\UserData\SharedFolder here u can put all of your pics and find them so easy on sdcard have nice day
An easiest way.. Download ES File Manager or Astro File Manager, copy the photos from the real location. Example SDCARD/Pictures/Line Then go to SDCARD and look for bstfolder and inside the folder...
install quickpic from the
Appstore! best and easiest way
thanks a lot for the info...it really works and now i can get all my data on my pc.. once again thanks :)
u can actually just drag a picture from your files and put it anywhere in bluestacks. there will be a pop up saying where to share with
i am using windows 7 what do i do to take photos from bluestacks
Hey....nothing to worry just install latest version of bluestacks(version 0.8.4.3036) and install ol file manager in it.....after that olfilemanager will give u a windows folder which includes...
Its very easy to copy the data from bluestacks to your PC. Just download Astro File Manager and install it using bluestacks. Its android browser. After installation, open it. You will get a folder...
may i delete some pic from
app which i hve save to send but this is private pic ,so i want to delete is after send but i have no this type option to delete my pic , these pics are save in...
go to all app click setting>>storage>>pictures and videos>>click to gallery and delete :)
There is no "SharedFolder" in the specified area in my sytem (neither in the C:\ProgramData\BlueStacks\UserData nor C:\Users(my username\etc\etc). I created a custom subfolder and named...
not able to delete pic of whatsapp from
Firstly install file manager open it after installing.You will see a folder named Whatsapp,open this folder.After opening this folder you should open the media folder then choose either the whatsapp...
U can copy the pictures from
to pc by this steps: 1:Select the required photos and copy it to the BstSharedFolder. 2:U will see the pics in the...
no need to worry in all such complicated processes.. to upload jst drag the pic file to the bluestacks window.. and to save a received
jst open it in the bluestacks wattsapp and then press the...
If you are using windows 7 then there is a simple step to copy your whatsap data to your pc.. 1. Go to C drive 2. Go to users 3. you can see the all user open it 4. there are 2 folder you can see...
Hi, Yes best way to go about it is ES File Explorer and copy pasting the Whatsapp
folder to bstfolder. You can then locate them under the same on C Drive!!!!
i use whasapp with bluestacks on my pc i am unable to downlaod any media sent to me by my friends
thank u soo much going to settings->storage->pictures and videos, worked out well...........
u dont need to download any soft..just drag the video and drop it into whatsapp(opened in bluestcks) thats it..!
thanks for the solution !!!..it works !! ;)
there is no folder 'userdata' in bluestacks folder in windows 7.
i dont have program data in my c: do i need to download instagram from blue stack to share my laptop
whats app? any easy way out?
go to all app click
setting>>advance settings>>storage>>pictures and videos>>click to gallery and delete :) this wiil work for damm sure
UserData link is for the files u sent .. not for the recieved files :) .. i still didnt see any right answer yet
Install ES File Manager it will help you
Search Qucikpic and install it.. it is the best and easiest app to copy any pic from whatsapp to your pc. try it before the installation of anyother file manager or explorer..
Simple and easy. Download ES File Manager in
Navigate to /storage/sdcard/Whatsapp/Media/WhatsApp Video Long press and mark the videos "More" settings in the bar below-----> Share...
To copy Pics from BlueStacks to your PC all you have to do is First Download Launch ES File Manager and search for the bstfolder and then go to folder Picture...copy the pics u want in picture...
Try this at the bottom right of the screen is a icon that looks like a < with 3 circles one at the each part Top right... left Middle and bottom right. Click it... That gives you the option to...
Thank you to All Answer I would like to add some info of what I did Today refering to your answers Install ES File Exploer on
Open the ES File Explorer, Go To :...
You may want to just search on your PC, if you have Windows 7 or 8. All the answers are correct, choose the one most convenient for you.
that is one easy task... first you need to have the following. 1.download es file explorer or any other file manager of your choice then install the application in bluestacks. after that you need...
More questions related to «How to download images in bluestacks»
content from Bluestacks.
— If you try to download content from Bluestacks, you can use the Dropbox way to...
I can't access
videos in Bluestacks.
— If you download a video using Bluestacks, you can access it by going to the...
Can't see
in folder.
— The Cubieboard website provides a guide on how to flash images on CubieTruck....
images look blurry and have a tiled effect. Why is this and how can I correct it?
— If an image has been upscaled, you can't modify it to bring the picture to an...
How to recover my old Bluestacks WhatsApp images?
— It's possible that the main files have been deleted through the update...
How to upload images from PC to Bluestacks Whatsapp?
— Install Bluestacks onto PC and launch Whatsapp. Open Bluestacks then choose...
Question variations related to «How to download images in bluestacks»
How to send images through bluestack?
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How to import images in bluestack?
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text:
Categories > Celebrities > My Chemical Romance > The Art Of Love
The Art Of Love
(#) Disenchantedxo 2010-02-06
haha bob is awesome :) i'm glad you update this alot, another one soon? xAuthor's response
Another one soon. When I can be bothered to get outta bed. (it's 09:15 am here) I'll get started on it. Should be out by the end or the day!
And yes, Bob is awesome, he's so quiet during interviews and he's very shy, but the expressions he makes are brilliant. Gotta love Bob
Thanks again!
LuThe Art Of Love
(#) wolf2008lllll 2010-02-07
Ha, your adding comedy! It's great. I love the all the deatail in your story. I've read fanfictions, with more detail, and length, ect... But the way you write is so minimal and short, yet its really effective.
I know what I mean... :)
To put it simply, its one of the best fics I've read in a while. As I've said before; I like to get my point across!
I really can't wait to see how it progress'.
Author's response
Thank you for the review!
I suppose I am adding a little bit of comedy, I think most of it will come from Bob and Frank, just because they seem the funniest out of MCR, not that the rest aren't.
And thank you for the comment about the detail, I try adding detail in but it seems to start droning on when I do so I keep it short.
Its weird to think that people like my story so much, to me it's not that good, maybe because I've written it myself , I have no idea.
I'm writing a new chapter now! While doing my h/wk, so it may take a longer time than before.
Thanks again,
LuThe Art Of Love
(#) EvieSaunders 2010-02-07
i like this chapter, always good to have a bit of frank in it xD
right on to read the next one!
xAuthor's response
tehe! thanks for the review,
Sign up to review this story.


text:
Mikey didn't really have any plans that day, well, apart from getting his car washed. But when he saw that chick working at the gas station, well, he started having plans immediately. Impressed with his ride, she was so easy to be taken home. And what a nasty one she was! With tats and boobs bigger than your head, she took every inch of Mikey's cock like a much-awaited blessing. Watch the lucky guy fuck her into the glass walls of his shower cabin! Hell, those things must be really fattie-proof for this sort of thing. Well done at BBW Bet!
Other blog post:
Facesitting Fatties
Chubby Pov


text:
Fan Art: The Ultimate Pop-Culture Alphabet, Plus: Pixar Characters Invade This Year's Best Picture Nominees
Take anything geeky, blend with a unique style, serve as something we would want to hang on our wall, and you have yourself a work of film nerd-approved art. Jeff Victor has created the ultimate pop-culture alphabet, featuring characters from cult hits like Star Wars and beloved franchises, such as Iron Man. The adorable illustrations can be seen in full on Victor's website.
If supercute characters from movies mashed together with something awesome is more your speed, check out these Todd Spence (from Break.com) Pixar posters. Taking figures from the animated giant's canon and matching them with this year's Best Picture Oscar nominees is a thing we wished we thought of first. Personal favorite: Carl Fredricksen from Up (voiced by Ed Asner in the film) stepping in for Bruce Dern's Woody Grant in Nebraska. Perfection.
In the movie The Edge of Seventeen, what is the name of the character played by Haley Lu Richardson


text:
Front Desk
Whether checking in or out or purchasing something from our market, the front desk is always here to serve you.
Lobby Lounge Seating
Enjoy additional seating near the bar, and unwind from a long day in Houston with friends or colleagues.
Whether enjoying breakfast or meeting colleagues in the lobby, we have plenty of space to accommodate everyone.
Accessible Queen/Queen Studio Suite
For guests with accessible needs, we have modified our Queen/Queen Studio Suites available.
Executive King Suite
For guests who prefer more space, our Executive King Suites are the largest rooms in the hotel.
Studio Suite - Living Area
All of our suites offer a living area, complete with a sofa bed, laptop tables and a full-size work desk.
Accessible Studio Suite - Living Area
We offer Accessible Studio Suites that feature a modified living area arrangement to accommodate our guests.
Accessible King Suite
For guests with accessible needs, we have our modified King Studio Suites available.
Communal Table
Enjoy ample seating throughout our lobby. Our Communal Table has built in electrical outlets so you are never without power.
Business Center
We offer a complimentary business center where guests can work on word documents, browse the Internet and print boarding passes.
Breakfast Buffet
Our free customized breakfast features all of your favorites: scrambled eggs, meat options like bacon, ham, sausage, fruit, yogurt, waffles, assorted breads and cereals and freshly brewed coffee and tea.
Lobby Bar
In our lobby bar, guests can order a variety of drinks and food. Our bar is open from 5:00pm until 10:00pm daily.
Complimentary Breakfast
For a healthy start to your day, enjoy our complimentary breakfast, featuring a variety of fresh and flavorful options. This photo is a representation of the hotel's facilities and amenities.
Healthy Breakfast Options
Our wholesomely delicious free breakfast includes Greek yogurt, which is fresh, healthy and filling, especially when customized with your favorite toppings from our toppings bar.
SpringHill Suites Breakfast Waffles
Wake up to breakfast favorites, including waffles fresh off the waffle maker, customized with your favorite toppings.


text:
See our updated Community Calendar for meeting dates and times
Absentee Ballot Application
For all Zoning and Permitting questions Contact Jason Farmer at firstname.lastname@example.com
Tax/Assessments Tax payments may be made in person on Mondays and Wednesdays, except legal holidays, from 4:30 - 6:30 pm, and the first and thrid Saturdays of the month from 9:00 am - 2:00 pm. Payments may be sent by mail or placed in the drop box located to the right of the main door at the township hall; your canceled check will be your receipt..»read more
Office Hours
Supervisor 3:00p-6:00p Mon Noon - 4:00p Wed
Treasurer 4:30p-6:30p Mon & Wed 9:00a-2:00p 1st & 3rd Sat
Clerk 10:00a-6:00p Mon & Wed
Assessor 8:00a-3:00p Wed
We've collected a list of helpful links to other websites that may be of interest to you. If you have a site you think should be added, you can also let us know. » CHECK MORE LINKS


text:
Wednesday, March 23, 2011
Olympus E-5: Absolute Sharpness
10 comments :
Your blog is always enlightening and inspirational. Hopefully one of these days I can book an appointment to have a shutter therapy session with you in Malaysia. Just don't charge me too much...hehehe.
Anyways just couple of questions...Did you use the same general setting when you first obtained your Olympus E5 especially when you did Macro photography? Do you also use a converter attached to your 50mm lens?( ie: Olympus EX-25 Extension Tube)to obtain such sharpness?
Thank you for your time and more power to your website!!!
PS. I made the mistake of posting this msg on your previous blog. SorryHello Eric,
Thank you so much for your support and comments, I appreciate them. When you do come over to Malaysia, do let me know, there is NO charge for shutter therapy sessions !!! I will bring you to the streets I usually go street hunting.
I actually set my Noise Filter to LOW for my reviews previously, and sharpness to +1, the rest of the general settings were the same. No I did not use extension tube, it was 50mm F2 macro alone. With the extension tube I could go a lot closer. However do take note that for my macro shots, many of them were executed with manual focus, especially the super close up ones.- Mehrdad3/29/2011 03:14:00 AM
Got my E5 for 1 week now and have some question. Although it's really sharp I see some disturbing artifacts in photos unless I reduce the sharpness to -2 and keep the NR on standard! Even on your shots I can see them. See the first shot one the mustache of your subject. What do you think? Hello Mehrdad,
That only means there were too much details being captured and the JPEG engine was working extra hard to squeeze everything out. Not very sightly I agree especially the compression artifacts, but they can be easily cleaned up. Usually I set to noise filter LOW and sharpness -1 should do the trick. I seldom see issues beyond that.- Anonymous1/05/2013 07:42:00 PM
Hi, Robin
nice review again!
I do have the e-5 (actually, one of your reviews made me buy it eventually), and I own the 12-60 SWD, 50-200 SWD, 9-18 and the 50mm.
Well, I am convinced that the order (from lowest sharpness to highest) would be somewhat like that : first we have the camera lenses (no matter the OEM), then the Medical Microscopes and better than those (by far) the Zuikos. :-) LoL
If only Oly could do better for the high ISOs.....


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Saturday, 21 February 2009
High Street pick of the week
Today you have two options courtesy of New Look- one is for now and the other is a S/S investment piece!
If you feel like adding some colour and fringing to your life then this purple shopper is a bargain at £10.00
You know you are going to succumb to polka dot even if you can't bring yourself to wear it. So, this bag will add 'on trend' moments into your life for £6.00!
9 comments:
Thank you for commenting, much appreciated. Sorry about no longer offering anonymous comments but spamming had become a very annoying issue. xxx
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Infectious Disease Control UnitMail Code: 1960 PO BOX 149347 - Austin, TX 78714-93471100 West 49th Street, Suite T801Austin, TX 78714
Phone: 512 776 7676 Fax: (512) 776-7616
This document has been designed to be a guide for medical care providers (physicians, nurses, pharmacists and laboratory workers) in the evaluation of possible exposures of humans to rabies virus and in the treatment of probable exposures. Information valuable to veterinarians and animal control officers who are submitting animal specimens for rabies evaluation is also included.
Rabies Prevention in Texas includes sections on: rabies biologicals; management of biting animals; collection and submission of animal specimens for rabies testing at the DSHS laboratory in Austin; list of and contact information for DSHS-designated laboratories; management of domestic animals exposed to rabid animals; rabies postexposure prophylaxis for humans with a guide for determining if it is necessary, plus a list of and contact information for laboratories that provide RFFIT (rabies titer) testing; preexposure vaccination for humans; adverse reactions, precautions, and contraindications pertaining to rabies vaccine; distribution points for human rabies biologicals; and contact information for the Zoonosis Control Branch and Infectious Disease Control Unit.
Rabies Prevention in Texas (PDF format) has been placed on the Internet so that professionals involved with evaluating and treating possible human exposures to rabies virus will have the needed information available to them everyday, twenty-four hours a day.


text:
Social Question
Were you an 80's baby?
Asked by whiteliondreams (1717)
June 14th, 2012
What things do you remember growing up as an 80's child?
Television series, food, candy, games, books, movies, music, and outdoor activities. What was your favorite of the things you remember? If you could go back, what date would it be?
I sure miss the eighties and wish I could go back to the days when I played outside with my friends and we pretended to be athletes, movie actors, and musicians. I remember going around the block to the fire hydrant after someone had opened it, on a hot summer day, and getting splashed until later on when the fire department would come and shut it off.
Observing members:
Composing members:
31 Answers
Answer this question
Have a question?
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text:
news, mugshots.com, unconstitutional mugshot laws, exonerations, arrest records, FBI most wanted
mugshot, mugshots.com news, mugshot news, editorials, opinion, mugshots
Misty Renee Hathcox
Who is Misty Renee Hathcox? Was Misty Renee Hathcox arrested? When was Misty Renee Hathcox arrested? What was Misty Renee Hathcox arrested for? Is this a mugshot of Misty Renee Hathcox? Are there more Mugshots of Misty Renee Hathcox? Are Mugshot laws constitutional? Who decides which Mugshots are Indexed? Why must Mugshots remain public mugshots and public record? Is this the Misty Renee Hathcox you know?
Misty Renee Hathcox was booked in Hillsborough County, FL.
All are presumed innocent until proven guilty in a court of law. Published mugshots and/or arrest records are previously published public records of: an arrest, an indictment, a registration, the deprivation of liberty or a detention. The mugshots and/or arrest records published on Mugshots.com are in NO way an indication of guilt and they are NOT evidence that an actual crime has been committed. For latest case status, contact the official law enforcement agency which originally released the information.
The following Official Record of Misty Renee Hathcox is being redistributed by Mugshots.com and is protected by constitutional, publishing, and other legal rights. This Official Record was collected from a Law Enforcement agency on 7/04/2012.
Mugshots.com ID: 7588375
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Submit a link now.
Not the Misty Renee Hathcox you were looking for? Click here to search for other Misty Renee Hathcox


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Science & Medicine | 68 |
The award winning Science Weekly is the best place to learn about the big discoveries and debates in biology, chemistry, physics, and sometimes even maths. From the Guardian science desk Ian Sample, Hannah Devlin & Nicola Davis meet the great thinkers and doers in science and technology. Science has never sounded so good! We'd love to hear what you think, so get in touch via @guardianaudio or [email protected]
December 7th, 2014
Episode 152 of 329 episodes
Roberto Mangabeira Unger argues that cosmology has wandered far from the disciplines of conventional science in its obsession with untestable speculationsIan SampleIain Chambers
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Caroline Feraday and Claire Bullivant on Brits in Hollywood, and Hollywood through the eyes of Brits.


text:
Linked by Daniel W. Steinbrook on Thu 7th Sep 2006 06:14 UTC
Syllable 0.6.1 is the latest incarnation of the operating system that "will be a reliable and easy-to-use GPLed operating system for the home and small office user" as their website states. That's quite a noble cause most other alternative operating systems never claim to be able to market to non-technical audience one day. Even getting Syllable up and running is pretty easy: fully-working VMWare images and a LiveCD images are provided for free download. Apparently, Danes are the primary downloader of the Syllable LiveCD, given the primary language in which the LiveCD page is by default.
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We'd love to use Firefox. All we need to do is port it. That would take about two man-months of work, which no one has right now. We need more developers, as always.
I'd love to see Thunderbird as well, but I much prefer Whisper (not simply because I wrote it). Whisper may have a few bugs, but it's still a nice email client (& the executable comes in at a whole 1010KB Large by Syllable standards but tiny compared to Thunderbird, Evolution, KMail etc.)


text:
Trolling Walleye: Seasonal Tactics DVD
Prices, promotions, styles and availability may vary by store and online.
- Additional discounts do not apply.
Hosted by Gary Parsons and Keith Kavajecz
Learn their signature crankbait designs
Techniques seen on "The Next Bite"
Improve your walleye trolling game
75 minutes
Experienced anglers Gary Parsons and Keith Kavajecz share everything on the "Trolling Walleye: Seasonal Tactics" DVD. They'll teach you their signature crankbait designs and spinner techniques as seen on their TV series "The Next Bite." Get all the tips, tricks, and techniques to improve your walleye trolling game. 75 minutes.


text:
Wichtige informationen
- Kurs
- Fernunterricht
- Dauer:
Flexible - Wann:
Freie Auswahl
Do you love animals and pets in general? Are you prepared to take you love one step further and become a qualified vet assistant! International Career Institute offers this course designed for those wanting to work in the pet care field.
A career in the animal care or pet care industry appeals to those with innate love for pets and animals in general. If you adore the furry and the cuddly and care for the untamed, you may want to consider rendering assistance to veterinary doctors. A vet assistant course from ICI can guide you in the steps necessary to be a reliable and successful veterinary assistant.
Anyone with a dream and desire to succeed in becoming a VET assistant.
Voraussetzungen: There are NO previous work or education requirements for entry into any course level. Students may enrol directly into the diploma or advanced diploma levels without completing lower ranked qualifications. Graduates can expect higher course levels to result in higher potential salary, positions and skill capabilities.
Veranstaltungsort(e)Beginn | Lage |
Freie Auswahl |
Distance Learning
Zu verbessern Everything OK.
Kurs abgeschlossen: April 2016 | Recomendarías este centro? Sí.
Zu verbessern Nothing bad.
Kurs abgeschlossen: April 2016 | Recomendarías este centro? Sí.
Zu verbessern Everything was good.
Kurs abgeschlossen: April 2016 | Recomendarías este centro? Sí.
Was lernen Sie in diesem Kurs?
Veterinary | Veterinary Nursing | Veterinary Assistant | Animal Nutrition | ||||||
Animal Behaviour | Animal Care | Animal Biology | Pet Care | ||||||
Industry | Pet Grooming | Veterinary science | Animal grooming |
Course modules:
Students will complete the following units:
· Introduction to animal and veterinary health care
· Animal health issues, illness and injuries
· Illness and disease identification and treatment
· Veterinary office management and administration
· Risk and safety management in animal care settings
· Animal first aid and injuries
· Preventative health care
· Pet / animal rehabilitation and nursing
· Routine health care treatments
· Anatomy and physiology
· Animal behaviour, perception and communication
· Animal genetics
· Animal social and instinct behaviours
· Animal handling and transportation
· Behavioural and abnormal animal behaviours
· Pet / Animal training
How Will You Benefit?
- Fast track your career as a pet car and veterinary assistant work
- Learn the inside secrets of how to become a pet care pro in months not years!
- Study a pet care / veterinary course that was designed in conjunction with senior members of the profession
- Free up valuable time, don't waste time and money travelling to classes
- Study at your own pace whenever and wherever you are
- Have access to a tutor who works in the industry you are training for
- Easy interest-free payment plans
Career Prospects
Veterinary assistants can take advantage of the increasing employment opportunities in this field. The number is predicted to grow even higher so by the time you finish the course, you can easily find a job as:
- Veterinary Assistant (veterinary technician, animal lab technician, veterinary assistant, animal hospital assistant)
- Pet Therapist, Groomer
- Pet Shop staff member or owner
- Animal Trainer, Groomer or Breeder (usually self employed)
- Animal Welfare Officer at Animal Shelters
- Zoo and Wildlife Park staff members.
ICI provides you with the information and training you need to be skilled in various aspects of the job. At the end of the course, you will be confident in helping veterinary doctors accomplish tasks like keeping records, examining animals for symptoms of illness or injury and even in giving comfort and support to pet owners when necessary. Enrol at ICI today and be a qualified vet assistant!
Stimmt etwas nicht mit diesem Kurs? Informieren Sie uns wenn Sie einen Fehler entdecken und helfen somit auch anderen Usern wie Sie.


text:
Canidae Life Stages Platinum Formula Canned Dog Food, 13-oz, case of 12
$23.88 FREE 1-2 Day Shipping over $49
You Save:
$2.11 (8%)
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As much as we love and care for our pets, they inevitably get older and often pack on a few extra pounds. CANIDAE created Life Stages Platinum Formula specifically with senior and overweight dogs in mind. It's great for senior dogs that need an energy boost and relief from joint pain, as well as adult dogs that have gained a few pounds and need an appetite-satisfying food that will keep them lean and healthy. High quality meat and fish, delicious flavor, and a scientifically formulated nutrient-rich recipe simmered in natural broth make this moist meal a great choice for your canine companion.
Key Benefits
- Real meat is the first ingredient in this quality high protein formula
- Reduced fat and protein levels for senior and overweight dogs
- No wheat, corn, soy, fillers, antibiotics, hormones, artificial flavors, colors or preservatives
- Antioxidants to promote a healthy immune system and overall health
- Omega fatty acids help promote a healthy and shiny coat
Special Note
CANIDAE recently updated packaging - Nothing in the recipes have changed. Their formulas are still the same quality nutrition you rely on to keep your dog healthy and happy!
Item Number47021
Food TexturePate
Breed SizeAll Breeds, Small, Medium, Large
Food FormWet Food
LifestagePuppy, All Stages, Senior, Adult
Made inUnited States
Special DietWeight Control, No Corn No Wheat No Soy
Nutritional Info
- Chicken, Chicken Broth, Lamb, Ocean Whitefish, Brown Rice, Pearled Barley, Sunflower Oil (Source of Omega-6 Fatty Acids), Eggs, Guar Gum, Dicalcium Phosphate, Potassium Chloride, Choline Chloride, Carrageenan, Salt, Psyllium Seed Husk, Flaxseed Oil (Source of Omega-3 Fatty Acids), Kelp, Rosemary Extract, Cranberries, Yucca Schidigera Extract, Lecithin, Cassia Gum, Glucosamine Hydrochloride, Chondroitin Sulfate, Vitamins (Ascorbic Acid (Source of Vitamin C), Vitamin E Supplement, Vitamin B12 Supplement, Vitamin A Supplement, D-Calcium Pantothenate, Vitamin D3 Supplement, Niacin, Riboflavin Supplement, Biotin, Pyridoxine Hydrochloride, Folic Acid, Thiamine Mononitrate), Minerals (Zinc Amino Acid Chelate, Iron Amino Acid Chelate, Manganese Amino Acid Chelate, Copper Amino Acid Chelate, Sodium Selenite, Calcium Iodate).
Caloric Content
- 900 kcal/kg, 327 kcal/g
Guaranteed Analysis | |
Crude Protein | 6.0% min |
Crude Fat | 4.5% min |
Crude Fiber | 2.0% max |
Moisture | 75.0% max |
Linoleic Acid (Omega 6) | 1.0% min |
Calcium | 0.15% min |
Phosphorus | 0.125% min |
Vitamin E | 50 IU/kg min |
Alpha Linolenic Acid (Omega 3) | 0.2% min |
Glucosamine | 150 mg/kg min |
Chondroitin Sulfate | 100 mg/kg min |
Feeding Instructions
If fed solely, feed one 13 oz can a day for every 20 lbs of body weight. Puppies may need up to twice the amount for the same body weight and nursing mothers up to three times. Fresh water should be available at all times.
To achieve a balanced diet and maintain firm stools, CANIDAE recommends a feed ratio of 25% canned to 75% dry dog food by weight.


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Cunningham, TN – On Saturday, April 12th, the Cunningham Volunteer Fire Department will be having their Annual Easter Egg Hunt at the Montgomery Central Middle School.
The Easter Egg hunt will begin at 10:00am.
Cunningham Fire Department's Annual Easter Egg Hunt.
The Easter egg hunt will be broken down into four age groups; ages baby-2, ages 2-4, ages 5-7, ages 8-10 and ages 11. There will be two grand prizes per age group. All kids will be given a ticket for a chance to win door prize at the end of the hunt.
There is no charge for the Easter egg hunt.
Montgomery Central Middle School is located at 3941 Highway 48 Cunningham, TN.
Bring the kids and family out Saturday for the Cunningham Volunteer Fire Department's Annual Easter Egg Hunt.
Cunningham Volunteer Fire Department was established in 1983 for fire protection in the Cunningham, TN area. Currently, there are 50 members of the department. Under the leadership of Butch McCurdy, Cunningham Volunteer Fire Department responds to fires, motor vehicle accidents with injuries, hazardous material scenes, emergency medical calls, fire alarms and carbon monoxide alarms.
They are supported by our community through donations and fundraisers so that they can continue to provide these emergency services.
For more information, visit their website at: www.cunninghamvfd.com
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text:
By: Christopher Moeller
Abstract: Explanation of Synchronize Output Directory default setting
When I manually place a .class file in the project's output path directory, it appears to get deleted when I build my project. Why is this happening?
When "Synchronize Output Dir" is turned on (Project Properties | Build | Java), all of the class files in the output path directory that do not have source files are deleted during compilation. This is the default setting. You should turn this off when you manually add classes to the output directory.
Server Response from: ETNASC02


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Model | HD-137 |
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Material | ABS |
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4 x Screws |
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Financial Forum Economics and Precious Metals |
Similar Threads | ||||
Thread | Thread Starter | Forum | Replies | Last Post |
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The Following 8 Users Say Thank You to ranger56528 For This Useful Post: | ||
The Government is responsible for the deficit of SS, not the older generation. Obama and crew .. what .. tripled the Nation's deficit in the past three years? Just imagine what the Healthcare will do when fully activated I am 61 and I will do everything in my power to AVOID signing on to that crap legislation health(death)care bill. IMO .. it is nothing but a sleazy way to get into our bank accounts and rob US with rising health costs to fund those who do not, never paid a dime into it because it's all about VOTES and POWER over US. |
The Following 2 Users Say Thank You to kris704th For This Useful Post: | ||
Just found this ...
http://online.wsj.com/article/SB1000...ditorialPage_h FOOD STAMP NATION Newt Gingrich threw a rhetorical tomato at the White House in Monday's South Carolina primary debate when he noted that "more people have been put on food stamps under Barack Obama than any other President in history." White House spokesman Jay Carney dubbed the claim "crazy" Tuesday, by which we assume he meant the President himself didn't literally enroll people in the program. Because any other way you cut the numbers, Mr. Gingrich is right. According to the U.S. Department of Agriculture's latest data, a record 44.7 million people participated in the Supplemental Nutrition Assistance Program in fiscal 2011 at a $75.3 billion cost to taxpayers. That's up from 28.2 million and $37.6 billion in 2008. Some four million people are now on food stamps in Texas, with California (3.7 million) and Florida (3.1 million) close behind. In absolute numbers, President Obama has presided over a food stamp boom. Mr. Carney says participation rates depend on the state of the national economy, which is true but not a sufficient explanation. As the nearby chart shows, the food stamp rolls have historically risen in recessions (1974-75 and 1981-82) and fallen during robust expansions (1980s and 1990s). The exception is the last decade, as the rolls rose as expected during the short 2001 downturn but then kept increasing throughout the decade with the single exception of a modest decline in 2007. The reason is policy as much as it is slower economic growth. President George W. Bush expanded food stamp eligibility with the egregious 2002 farm bill by adjusting deductions and income tests and encouraging states to find more participants. The Pelosi Congress then rebranded the program in the Food, Conservation, and Energy Act of June 2008 to emphasize nutrition rather than the stigma of receiving handouts. As a result and also thanks to the recession in 2008, food stamp participation rose by 10.9 million across the Bush Presidency. But Mr. Obama is now outstripping that dubious achievement, and in only three years rather than eight. The 2009 stimulus bill increased maximum benefits and authorized more money for states to administer the program. Some 11.2 million more people have joined the rolls from 2009 through 2011, including 4.4 million last year during what was ostensibly an economic recovery. Food stamps were intended to help the truly needy so children wouldn't go without nourishment, but Washington is slowly turning it into one more entitlement for millions of Americans. Mr. Gingrich received loud applause on Monday less for his partisan criticism than because he zeroed in on the dangers of dependence on government, especially for the poor. He said he wanted to "help poor people learn how to get a job, learn how to get a better job and learn some day to own the job." This is the right political goal. What's "crazy," to borrow Mr. Carney's word, is for a society to shrug its shoulders when nearly 45 million citizens are on the food dole. Obama racking up MORE National debt !!! This has NOTHING to do with Baby Boomers. We see this for what it is .. more people on the dole NOT contributing and our Youth will suffer for it. My oldest daughter just gave birth to her third son this morning. YOU BET I CARE ABOUT THE WELFARE FOR ALL OF MY GRANDCHILDREN IN THE YEARS TO COME .. and the mess we .. and YOU YOUNG VOTERS are heaving upon them. |
I read that if any candidate tries to mess with the elderly people's entitlements that it's like touching the third rail politically. Ron Paul says he would still take care of the elderly but those baby boomers want the maximum amount of money that they can squeeze out of broke ass uncle Sam as possible.
If it was just people under age 40 voting Ron Paul would win the Republican primary easily. But because of the older baby boomers reaching the age where they will start collecting SS he is unelectable. Young middle class Americans are trapped with an unchangeable corrupt government. There is no difference between the two parties now. Both are controlled by money printing criminal bankers and both parties cater to their own type of parasitic constituents. Conservatives have retired and become liberals now. Government come and take care of me! Give me my money! You owe me! Most conservatives have aged out or will be soon and now many of them have to rely on the government. The core principle of conservatism is self reliance. What do you do when millions of conservatives have aged and can no longer support themselves and have to resort to big government which is the core of liberalism? I predict America will be becoming increasingly socialist to take care of all those boomers. The health care system will have to become socialist to take care of those millions of sickly smelly old farts. Young people will live like the communist Eastern Europeans did. Working a crap job for low wages. Never being able to own a house or land and living in crappy little apartments. Driving crappy cars, wearing cheap clothes, they will have less freedoms than their parents did. Multiple generations of family crammed into tiny apartments. Massive unemployment, going to college but never being able to find decent employment being stuck doing menial jobs............all to pay for those greedy baby boomers. The average food stamp benefit is like what? $150 a month A baby boomer on Soc Sec gets a little over $1,200 a month not to mention their massive Medicare bill for all the health problems they have. |
The Following 2 Users Say Thank You to Wayfara For This Useful Post: | ||
Ron Paul is keeping himself from getting elected. He is bat %$# crazy. 0% tax... really Paul... really. What are you going to do trade pelts to China to pay off the debt?
The Following 4 Users Say Thank You to Wigglesdabum For This Useful Post: | ||
We only want OUR $$$$$ back!! Why don't you get that ??? |
The Following 3 Users Say Thank You to kris704th For This Useful Post: | ||
Ok 3 pages and no answer on the college thing....I think silence has given me the answer....probbaly those evil baby boomer parents paid for it...
major? liberal arts, music, women's studies, or art...those are my guesses |
he cant see it because he, himself, is too selfish to understand that just because he wants rp, that doesnt mean the rest of us do. |
The Following 4 Users Say Thank You to tdawg75 For This Useful Post: | ||
I hope for Pauls sake, he never asked the OP to serve on the PR campaign side of it.....
he would lose support quicker than a rat out of an aqueduct (Life of Brian - Monty Pyhton) |
As Judge Judy would say *Open your ears, and listen. You might learn something* |
The Following User Says Thank You to kris704th For This Useful Post: | ||
I am the platypus to her labeling system I guess |
No I don't think illegals or drug addicts etc should get a free ride so to the person who is painting everyone with the same brush, please do some more research. |
The Following 4 Users Say Thank You to Cinbad For This Useful Post: | ||
In law school, I received a substantial scholarship of about $15,000 per year from a private school for my performance at my undergraduate institution. I then borrowed approximately $100,000 from private lenders to pay for my bills. I purposefully avoided any and all government loans because I believe no one else should pay for my education. I was able to pay all my loans off within three years after school by starting my own business and working my rear end off. To generalize and suggest that every individual from my generation is lazy and dependent is wrong. I work hard every single day in my own business that I built from the ground up. I take pride in my accomplishments considering I came from next to nothing. I realize how fortunate I am, and because of my success, I have chosen to share my skills with those who could not otherwise afford it. I have dedicated more than 1,000 hours of my time during EACH of the past three years helping veterans coming back from these undeclared and unconstitutional wars that suffer from physical and mental illnesses and have no jobs waiting for them. Our government treats them like crap, and I refuse to sit on the sidelines and let it happen. When the older generation of lawyers cast them away because they are unable to pay, I am there to assist them no matter the cost or the time involved. I am not lazy, I am not a dependent, and I am perfectly capable of caring for myself. |
The Following 3 Users Say Thank You to DollreeMappEsq For This Useful Post: | ||
But, you were not the one who labeled basically everyone NOT ayoung, white, and with a job as the reason America hates Paul SHE has not answered the college question and it speaks volumnes. Oh ya, and I am NOT a baby boomer.......just tired of the same crap every 4 years with people expecting something special and supernatural to happen with the next flavor of the month candidate. I am prepped, so is my family...wanna know what I want? sure, no problem burn DC, f'in BURN |
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Important Dates
Grading @ Hombu - 10th-6th Kyu Sun Apr 02 @10:00AM - 12:00PM |
Grading @ Hombu - 5th-2nd Kyu Sun Apr 02 @12:30PM - 02:30PM |
Do you have a great photo or video from a BKK class or event? Send an email to the webmaster! (See Contact Us page) |
Budo Kenkyukai |
The Australian Martial Arts Research Network is a progressive Martial Art with time-honoured values. Budo is a discipline of austere training which promotes etiquette, skillful technique, physical strength and the unity of mind & body. The object of Budo is to cultivate character, enrich the ability to make value judgments and foster a well disciplined and capable individual through participation in physical and mental training utilising martial techniques. The technical foundations derive from over thirty years of active study into Kempo, Karate, Thai Boxing, Ju Jitsu and more recently, Amok Edged Weapons Solutions. Today's media constantly reminds us of the need for an effective method of self defense. BKK's primary focus is to utilise realistic martial arts techniques and strategies that provide Men, Women & Children with the appropriate harm minimisation and conflict resolution skills to deal with situations that call for immediate and effective action. Dojo Kun (Oath) "See the value in all arts and continue to retain an open mind" Mission Statement A Dynamic organisation of highly skilled, accredited Instructors committed to educating, motivating and developing students of all ages in the martial art that is Budo Kenkyukai. Vision Statement Men, Women & Children who are physically and mentally healthy, self-confident and able to resolve conflict situations safely. |


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Become a member!
Trailer: Hanna
Photo: Hanna
Hanna (Saoirse Ronan) is a teenage girl. Uniquely, she has the strength, the stamina, and the smarts of a solider; these come from being raised by her father (Eric Bana), an ex-CIA man, in the wilds of Finland. Living a life unlike any other teenager, her upbringing and training have been one and the same, all geared to making her the perfect assassin. The turning point in her adolescence is a sharp one; sent into the world by her father on a mission, Hanna journeys stealthily across Europe while eluding agents dispatched after her by a ruthless intelligence operative with secrets of her own (Cate Blanchett). As she nears her ultimate target, Hanna faces startling revelations about her existence and unexpected questions about her humanity.
Peter Travers Rolling Stone
Once upon a time, a widowed father (Eric Bana) kept his feral daughter, Hanna (Saoirse Ronan), in a remote Finland shell, teaching her to hunt, fight and kill like an assassin. Social skills, not so much. Now, at 16, Hanna is pushed into the real world and the dangers of wicked witch Marissa (Cate Blanchett, superb), a CIA agent who shares dark secrets with Hanna's dad. And so begins a fairy tale of lightning speed, gritty action and shocking gravity, all driven by the electronic beat of a hypnotic score by the Chemical Brothers. The gifted Brit director Joe Wright excels at knocking you off balance and forcing you to rearrange his puzzle pieces in your head. Out of a script by David Farr and Seth Lochhead, Wright (his Atonement won an Oscar nod for Ronan) carves a surreal fable of blood and regret. Ronan is an acting sorceress, and her scenes with the excellent Bana cut bone-deep. As Hanna is pursued across Europe, hoping to reunite with her father in Berlin, we watch her in the act of inventing herself. A funny, too-brief encounter with an English family on holiday lets Hanna talk boys with their daughter (sass queen Jessica Barden) and almost annihilate one lad who dares to make a move. But as Hanna confronts her past, the movie becomes like nothing you've ever seen. I'd call it a knockout. Rolling Stone Copyright Rolling Stone
Dance Academy: The Movie Now Showing Rated PG (MILD THEMES), 101 mins
The Lego Batman Movie Now Showing Rated PG (Mild themes and animated violence), 104 mins
Ghost in the Shell Now Showing


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Subject: | DO NOT REPLY [Bug 46124] AnimationEngine.removeAnimation doesn't clean up the "animation sandwich" correctly. |
From: | |
Date: | Wed, 5 Nov 2008 19:55:19 -0800 PST |
https://issues.apache.org/bugzilla/show_bug.cgi?id=46124 Cameron McCormack <cam@xxxxxxxxxx> changed: What |Removed |Added ---------------------------------------------------------------------------- Status|NEW |RESOLVED Resolution| |FIXED --- Comment #3 from Cameron McCormack <cam@xxxxxxxxxx> 2008-11-05 19:55:18 PST --- Hi Steve and Helder. Thanks for the bug report, and the attached patch and test. The proposed solution doesn't completely solve the bug, however. When an animation is removed, its effect needs to be removed also. This is meant to be achieved by leaving the Sandwich object in there, with no animations in it but with shouldUpdate = true. The ANIM_TYPE_OTHER case is just missing a check in tick() to ensure sandwich.animation is not null (as is checked for XML/CSS animations earlier in the method). This is now fixed in r711765 ( https://svn.apache.org/viewcvs.cgi?view=rev&rev=711765 ). -- Configure bugmail: https://issues.apache.org/bugzilla/userprefs.cgi?tab=email ------- You are receiving this mail because: ------- You are the assignee for the bug. --------------------------------------------------------------------- To unsubscribe, e-mail: batik-dev-unsubscribe@xxxxxxxxxxxxxxxxxxxxxx For additional commands, e-mail: batik-dev-help@xxxxxxxxxxxxxxxxxxxxxx |
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Marketing Information
The VS0201 2-Port VGA Switch with Audio allows you to connect two audio/video source computers to a single monitor or projector with full audio and quickly switch between the devices for use.
VS0201 built-in bi-directional RS-232 serial port for high-end system control, and advanced RS-232 control. The unit �s bi-directional RS-232 serial port also allows serial devices, such as touchscreens and barcode scanners to be included in the installation. With the ability to use high-end serial control devices via RS-232 commands allows the VS0201 to be controlled by secondary systems with advanced functions such as; port switching, auto switching, front panel pushbutton lock, and Power on Detection. The VS0201 is perfect for the meeting room, media centers, class rooms, or any presentation environment requiring multiple audio/video sources to be accessed on a single display.
General Information
Product Type | Audio/Video Switchbox |
Manufacturer Part Number | VS0201 |
Manufacturer Website Address | www.aten-usa.com |
Manufacturer | Aten Technologies |
Product Name | 2-Port VGA Switch with Audio |
Brand Name | Aten |
Technical Information
Connectivity Technology | Cable |
Number of Displays Supported | 1 |
Number of Input Devices Supported | 2 |
Graphics Modes | VGA |
Maximum Video Resolution | 1920 x 1440 |
VGA In | Yes |
Number of VGA Inputs | 2 |
Media & Performance
Maximum Operating Distance | 213.25 ft |
Physical Characteristics
Weight (Approximate) | 15.87 oz |
Height | 1.7" |
Width | 3.0" |
Depth | 5.1" |
Package Contents |
Green Compliant | Yes |
Green Compliance Certificate/Authority | RoHS |
This product is shipped only within Canada and Warranty valid for Canada only.
Computer Valley makes every effort to provide accurate specifications, descriptions, images and price on all products that are found on www.computervalley.ca
However for complete and updated descriptions, compatibilities, specifications and actual photo we recommend our clients to consult with manufacturer's websites or simply call us for proper clarifications. Computer Valley is not responsible and will not accept returns for items purchased that are not compatible with your system. Please ensure that you have verified compatibility and actual photo with your system by visiting the manufacturer's web site prior to purchase or give us a call to make sure what you are purchasing.
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There' s a couple of 3'' on the sales by induviduals. There is also a dealer on Rick's site that has a non 3.5" in stock. Non flipper, Slicer grind, Just a heads up.
Last edited by Oldbugr; 09-22-2012 at 08:07 PM.
Are you telling me I'd have to pay about $800-$900 for an Xm-18 even from a dealer? Yikes.
If the collaborations interest you...get a little Kershaw Cryo. It's worth every penny. And also consider the ZTs.
But, knowing that collaborations are a success, if you don't like the current offerings I'm betting new stuff will be a reality at some point. For example, I'd like to see a smaller version of the 0560/0561.
0560 and XM 18 3.5
0550, 0560, Endura, Delica...
Top to bottom...
I meant to say non flipper- Sometimes the fingers don't do what the brain says to do.
Thanks very much for posting those pics! Heck of a collection! Congrats!
I don't know what to say at this point - I'm undecided on Hinderer. Unsurprisingly, the dealers I've contacted so far pretty much said: "good luck". Oh, well... No big deal at the end of the day. I won't pursue it.
The Zt-30X line up looks cool.
Thanks all for your input!
I wouldn't give up so easy Sufler, patience and due diligence will pay off.
When the first 3" production XM's started hitting the secondary market from the most recent batch the prices were hitting around 900, look around now, recently seen them brand new in box going for a low as 600 on this board.
If you are determined enough it will happen and don't forgot the ZT collaborations posted above.
There are currently 1 users browsing this thread. (0 members and 1 guests)


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Which Joe gave his name to 'sloppy joes'? We look at five interesting sandwiches and their lexical origins.
Definition of Blenheim Orange in English:
Blenheim Orange
An English dessert apple of a golden or orange-red variety which ripens late in the season.
- 'Another favourite was Blenheim Orange which was often found in Christmas stockings just when the flavour was at its most rich and distinctive.'
- 'The Blenheim Orange is one of the loveliest apples of all with its dry distinctive flavour.'
- 'They must has been one of the first nurserymen to distribute Blenheim Orange, as it was not in wide circulation before 1818.'
- 'When scaffolders uprooted his Blenheim Orange, Nigel Slater swallowed hard and set off to find the perfect apple tree.'
- 'The first cottage after the mill has a plaque recording the raising of the first Blenheim Orange apple here by George Kempster.'
So named because it was discovered growing by the boundary wall of the Blenheim estate (see Blenheim) in the 18th century.
Further reading
6 punctuation marks you might be using incorrectly
Read moreWe take a look at several popular, though confusing, punctuation marks.
An A-Z of country name origins
Read moreFrom Afghanistan to Zimbabwe, discover surprising and intriguing language facts from around the globe.


text:
Republicans in the House and Senate introduced legislation Thursday that would establish a new trigger to block the Affordable Care Act's contentious employer mandate from taking effect next year.
Already the subject of multiple hiccups, the ObamaCare provision requiring companies to either offer their workers healthcare insurance or pay penalties would be subject to additional delays under the so-called Certify It Act.
A finding that the mandate would have a negative impact on small-business health insurance premiums or job creation would delay its enactment for the following year, the lawmakers said.
"Republicans want to repair the damage ObamaCare has done and prevent future damage," Alexander said. "This bill says, 'Let the facts speak for themselves — if premiums are going up and jobs are being cut, then delay the mandate.' "
The mandate requires that firms with more than 50 full-time workers offer health insurance or pay a penalty. Its backers contend the requirement is crucial to the Affordable Care Act's overall scheme of expanding access to healthcare coverage.
The administration has pushed back the mandate's starting date by a year, however, to January 2015.
Some businesses got an additional reprieve earlier this year, when federal health officials announced that employers with between 50 and 99 workers have until January 2016 to offer health insurance or pay a fine.


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7 Vain and deceitful is their
curses fill'd, and lies;
By which the mischief
of their heart
study to disguise.
8 Near public roads they lie
all their art employ,
The innocent and
poor at once
rifle and destroy.
9 Not lions crouching in their
Surprise their heedless prey
With greater cunning, or express
More savage rage than they.
10 Sometimes they act the harmless
And modest looks they wear;
That so, deceiv'd, the poor may less
Their sudden onset fear
11 For God, they think, no notice
Of their unrighteous deeds;
He never minds the suff'ring poor,
Nor their oppression heeds.
12 But thou, O Lord, at length
Stretch forth thy mighty arm,
And by the greatness of thy pow'r,
Defend the poor from harm.
13 No longer let the wicked
proudly boasting, say,
God regards not what we do;
never will repay."—Common Prayer
* * * * *
1 Shall I for fear of feeble
The Spirit's
coarse in me restrain?
Or, undismay'd
in deed and word.
Be a true witness
of my Lord.
2 Aw'd by mortal's
frown shall I
Conceal the word
of God Most High!
How then before
thee shall I dare
To stand, or how
thine anger bear?
3 Shall I, to sooth th'
unholy throng,
Soften the troth,
or smooth my tongue,
To gain earth's
gilded toys, or flee
The cross endur'd,
my Lord, by thee?
4 What then is he whose scorn
I dread?
Whose wrath or
hate makes me afraid
A man! an heir
of death! a slave
To sin! a bubble
on the wave!
5 Yea, let men rage:
since thou wilt spread
Thy shadowing
wings around my head:
Since in all pain
thy tender love
Will still my
sure refreshment prove.
Wesley's Collection.
[17] See Dr. Torrey's Portraiture of Domestic Slavery in the United States, page 85-86.
[18] Among the English, our real friends and benefactors.
[19] In the first edition of this work, it should read 1816, as above, and not 1826, as it there appears.
[20] "Niger" is a word derived from the Latin, which was used by the old Romans to designate inanimate beings which were black, such as soot, pot, wood, house, &c. Also, of animals which they considered inferior to the human species, as a black horse, cow, hog, bird, dog, &c. The white Americans have applied this term to Africans, by way of reproach for our color, to aggravate and heighten our miseries, because they have their feet on our throats, and we cannot help ourselves.


text:
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Light Green Bowel Movements
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December 12th, 2011
What could be wrong? I had light bright bowel movement. Also I feel weak, stomach is cramping,
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Deepak Anvekar
replied December 26th, 2011
Hello and welcome to e health forum.
Loose green stools and abdominal cramps can be due to various causes including parasitic infections (Giardiasis), malabsorption syndromes (lactose intolerance), indigestion, dysentery, etc.
A proper medical history and a complete physical examination will be very important to know the actual cause of symptoms.
For the time being treatment with probiotic supplements, digestive enzyme replacement therapies and âproper diet changes ( low fat, low carbohydrate, with increased fibre content as well as rich in fruits âand vegetables) will be very helpful to improve the digestion as well as to reduce all the symptoms.
You might consult with a gastroenterologist who can be able to examine you and advice proper âmedications as needed.â
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text:
The primitive doctrine of justification investigated: relatively to the several definitions of the church of Rome and the Church of England; and with a special reference to the opinions of the late Mr. Knox, as published in his remains by George Stanley Faber.
Main Author: | Faber, George Stanley, 1773-1854. |
Language(s): | English |
Published: |
London, R. B. Seeley and W. Burnside, 1839. |
Edition: |
2d ed.: |
Subjects: |
Knox, Alexander,
> 1757-1831.
Justification. |
Physical Description: |
lxiv, 514 p.
24 cm.
Locate a Print Version: |
Find in a library |
- Full view (original from Harvard University)