text:
If you are considering picking up the MyTouch 4G handset on T-Mobile, you'll definitely want to check out the latest Ad that T-Mobile has put out for it, as it takes a direct swipe at both Apple and AT&T.
Usually, if a rival company takes a swipe at another company, they often do it in a discrete fashion, but T-Mobile defnitely don't hold back in their remarks about Apple and AT&T.
Firstly they criticize AT&T's 'slow' 3G network speeds on the iPhone 4, and then they have a further dig at Apple by making fun of the Facetime Video Chat on the iPhone 4, and the fact that it can only handle WiFi calling at the moment.
It is pretty funny I have to say, and very surprising coming from the likes of T-Mobile, who mention more than once that their 4G network is now the largest 4G network in America. – Sprint, we're looking at you.
Follow us on Facebook, Twitter or Google Plus.
Also See: New iPad Pro 12.9, Mini release date rumors at April event


text:
Home | Forums | Rules | Articles | diyAudio Store | Gallery | Wiki | Blogs | Register | Donations | FAQ | Calendar | Search | Today's Posts | Mark Forums Read | Search |
Everything Else Anything related to audio / video / electronics etc) BUT remember- we have many new forums where your thread may now fit! .... Parts, Equipment & Tools, Construction Tips, Software Tools...... |
Please consider donating to help us continue to serve you.
Ads on/off / Custom Title / More PMs / More album space / Advanced printing & mass image saving |
Thread Tools | Search this Thread |
21st August 2007, 04:01 PM | #31 | |
diyAudio Member
Join Date: Mar 2004
Location: Monroe, WA
21st August 2007, 04:20 PM | #32 |
diyAudio Member
Join Date: Apr 2007
Location: Kingston WA>
Funny place, Port Townsend... When my family and I were moving to Washington, we took a break from house hunting in the Seattle area to attend the Wooden boat fest... We stayed for 6 years
7/10 |
24th August 2007, 10:10 PM | #33 |
diyAudio Member
Join Date: Mar 2006
Location: Pacific NW
Anybody want to carpool on the Ferry??? I am shooting for the 8:55 am to Vashon. I will be driving down from Bellingham in the morning, so I can go with someone, or take someone - either way.
Edward |
25th August 2007, 12:09 AM | #34 |
diyAudio Editor
My Dad grew up on Vashon in the late 30's !
Now just because you are having this event doesn't mean that you can't come visit us too in San Francisco at BURNInG AMP!!! I've got a boat too, but it's plastic... It's an Olson though Terry! Possible boat rides in S.F. bay for the hardy!!! |
25th August 2007, 02:28 AM | #35 | |
diyAudio Member
Join Date: Nov 2002
Location: Seattle,Wash.
It may seem strange, but I've got a San Francisco Great Pelican. Just an extended and widened SF Pelican with a cabin. Battened Junk-rigged sails, it needs a fair amount of work though, I guess it's waiting for me to retire. As a "side note," the Central Sound DIY Meet is TOMORROW(Saturday) on Vashon Island. I hope that some of you that are undecided will elect to show up. These meets are a lot of fun and very laid back. Now, having said all that, I can assure you that it really feels good to be back on topic, complete with a clear conscience. Best Regards, TerryO
"If you have to ask why, then you're probably on the right track." quote from Terry Olson's DIYaudio Forum application |
25th August 2007, 06:58 AM | #36 |
diyAudio Editor
Yeah guys, go. It's fantastic to meet your fellow fanatics...
The S.F. Pelicon looks a little like an El Toro on mega steroids. I know that there have been various sizes. At a fun race once We passed one and it had 2 12 yr old girls in it- moving along on SF Bay looking perfectly safe, with that little bowsprit and high gunnels... gaff rig...a lovely sight... From what I've read, the original was developed by a guy for exactly that - a boat his kids could take out in the bay in some safety... Few people around here even know much about them... |
26th August 2007, 05:19 AM | #37 |
diyAudio Member
WOW, what fun!
Bud |
26th August 2007, 07:02 AM | #38 |
diyAudio Member
Join Date: Nov 2002
Location: Seattle,Wash.
I hope everyone made it home safe and sound from Vashon Island. I can't speak for anyone except myself, but I thought it went exceedingly well.
I'd like to extend my heartfelt thanks to Lew Hardy of Vivaldi Audio, Doc Bottlehead and Jerry Pomeroy, President of the Pacific Northwest Audio Society for all their hard work in bringing this together. I also like to thank one of the Northwest's most prolific Speaker Builders, John Nail (and his wife Rhonda), for making the long drive from Eastern Washington to the meet and for offering to measure people's speakers. One of the builders actually was able to correct a phase problem with his speakers, right at the meet, because of the frequency response measurements that John performed. The Speaker Builders made a big turnout and there were a lot of different designs to listen to and compare. Nice work Guys!!! I had some favorites, but since I built every one of them, I won't bother going into greater detail. OK, maybe there were a few (?) that were as good as mine, but I've always prided myself that "nobody" ever got better sound for less money than I could, but it seems that several people may have done just that. The other, totally unexpected, development was that Mini-Speakers made an appearance in force. There were a number of speakers driven by 4 and 3 inch drivers (several fullrange and a number of 2-ways) that sounded, IMHO, absolutely phenomenal. Best Regards, TerryO
"If you have to ask why, then you're probably on the right track." quote from Terry Olson's DIYaudio Forum application |
26th August 2007, 01:18 PM | #39 |
diyAudio Member
Join Date: Jul 2003
Location: Seattle, WA
After the last PNAS speaker contest and this meet I think we need a mathmatical expression for Cost/Listening Pleaure.
I got the impression that the middle ground for audio seems to be rapidly disappearing. Good sounding speakers are either very expensive and complicated or very inexpensive and simple. Joe's Bowling Balls blur the lines at bit however. Also, VSAC is alive and well. |
26th August 2007, 03:03 PM | #40 |
diyAudio Member
Join Date: Jul 2003
Location: Seattle, WA
A few pics
The good pictures will likely be published in the next Pacific Northwest Audio Society Newsletter. Here's some quick snapshots in the meantime.
Here are Joe's Balls. |
Thread Tools | Search this Thread |
Similar Threads | ||||
Thread | Thread Starter | Forum | Replies | Last Post |
Audio Meet in Richmond, BC | jamite | Everything Else | 62 | 28th January 2008 02:38 AM |
Audio meet. | castlesteve | Full Range | 0 | 10th January 2008 01:42 AM |
P-audio 15 coax - how do they sound? - do they meet spec? | freddi | Multi-Way | 2 | 18th January 2007 04:58 AM |
DIY AUDIO Meet in NY/NJ ? | jackinnj | Everything Else | 0 | 21st August 2004 06:10 PM |
NWWA DIY Audio Meet 2004 | Timn8ter | Tubes / Valves | 0 | 8th March 2004 01:13 PM |
New To Site? | Need Help? |

subset: commoncrawl


text:
#15263 closed defect (bug) (fixed)
Several Template Hierarchy areas a broken
Reported by: | thee17 | Owned by: | scribu |
Milestone: | 3.1 | Priority: | normal |
Severity: | critical | Version: | 3.1 |
Component: | Template | Keywords: | needs-patch |
Focuses: | Cc: |
I think I found a problem in trunk. If you category-term.php in a there does not work, category.php does, I have tested author, taxonomy, and tag with similar results. I was able to duplicate on 2 sites running the most recent trunk. I think the problem is in [15611]. When I revert the wp-includes/theme.php back to revision 15610 it works as expected.
Change History (9)
6 years ago
- Keywords reporter-feedback added
Could you post more detailed steps to reproduce?
6 years ago
Take a category, tax, or tag:
(using categories because it is more likely you have one to test. For this example the catagory ID is 5 and slug is test)
Create a category.php to your theme visit your category test and the template is your category template.
Rename it to category-5.php or category-test.php and the visit your category and you will see it use your archive.php or index.php rather than your template.
mrmist was also able to reproduce in #wordpress IRC today.
6 years ago
- Cc admin@… added
- Keywords needs-patch added; reporter-feedback removed
get_query_var( 'cat' ) returns a blank string on the category archives page.


text:
Sounds simple, right. Enjoy this Day is the advice this little bird carries. Just a reminder, in our jam packed days to live in the moment. The second side message is your choice, choose from 25 words and messages.
This petite 2-sided pendant features art plus a message of your choice. Images are encased on both sides in ultra-clear domed glass and set in a lead-free pewter bezel. Purchase includes 30" antique silver ball chain. It slips over your head (or you can open the connector), and is easy to shorten with the snip of your scissors. These pendants are not waterproof.
Outside dimension (not including bail) - 3/4 inch diameter. Inside 5/8" diameter; 1/2" deep.
Your purchase will be sent via USPS. Choose First Class or Priority Mail. Calculate rates at checkout. Orders under $50 always ship for flat rate $5.00 fee.
Your piece will arrive on an insert card featuring a vintage graphic background and sealed in a cello bag. If you would like your purchase to be gift-ready, order the gift box with vintage style insert. Be sure to include a message if you would like a gift tag attached to either style packaging.


text:
Description: Hottie, Hannah Harper, has got her nice juicy mouth around this dude's big ballpark frank. After blowing the guy, she gets her pussy wickedly tongue fucked before having her cunt stuffed full of bococky. Tags: Blonde, Ball Sucking, Blowjob, Big Boobs, Cock Riding, Pussy Licking, Fingering, Rimming, Facial, Finger Banging, Hardcore, Deep Throat, Cumshot, Pussy Fucking Site: Raw Tube Category: Blonde Views Since The Last Update: 26262 Discuss: CommentsSpecial: Free Membership
Share Video: press ctrl+c to copy and ctrl+v to paste Embed Video: Link To Video:
Rating: 2.5/5 (76 votes cast)


text:
2 comments:
- Vicky said...
Well well, I am an insomniac too (for some reason i don't find this coincidence surprising) so have been reading about your flaxseed experiments with interest. Also checked out your link to Seth's blog post on it. Do you really think it's making a difference? And where are you buying it from? And if it tastes so disgusting, why aren't you doing the oil capsule thingy? Sorry for all the questions...
- MEM said...
Heyyyyy vicky. No problem with the questions...
Do I really think it's making a difference? Hard to tell. I'm doing so many things "right" at the moment, it's hard to be sure about strict cause/effect relationships.
I will say that the past few nights I've taken flaxseed before bed, and no other sleep aid (i usually use a mix of melatonin and valerian), and have slept through the night, which almost never happens.
But, I've also been exercising, standing all day, not having much caffeine, not eating after 7pm or so, no alcohol, etc. So, yeah...hard to say.
I bought mine at De Avondmarkt, but any natuurwinkel will have them (as lijnzaad).
And I'm not doing the oil capsule b/c I'm in it for the fiber as well, plus the oil is missing some of the critical cancer-fighting stuff that the seeds have... - 2:20 PM


text:
7 Responses to "'they're bussing in palm trees!'"
March 3, 2011 at 4:23 pm
I have to watch O'Reilly every once in a while and as luck would have it, there was the video, with palm trees!! I pointed this out at the time and we decided it was another FartBart ploy. Anything to discredit the other side. I especially liked when "WE" were accused for playing dirty trix. BTW, the WI legislature want to put thru an ordinance or law on 'phony' phone calls. Can you believe? Why didn't we think of that when FartBart was playing?
March 3, 2011 at 4:49 pm
I want to share a wonderful portion of a comment made on krugmanblogs.nytimes.com. I thought it might be prophetic. The commenter wonders what the tipping point was in Egypt and then writes "did the cognitive dissonance between official narratives and daily life become untenable for the non-ideological center? At some point propaganda ceases to pacify and instead incites anger, the intended scapegoats suddenly inspire empathy instead of scorn". I really think this is starting to happen here.
March 3, 2011 at 9:06 pm
Hhhmm. We'll see because at 74% likeability of the President, even with all the misinfo and lies about policy, things may be getting a bit clearer to some folk previously in the fog of apathy.
March 3, 2011 at 11:58 pm
It can't hurt that the likes of Huckabee are telling bold-faced lies. If the right wing is so right in their arguments, why do they need to lie, cheat and steal? Wouldn't the truth be enough? Even a fool has to start asking that question.
March 3, 2011 at 8:22 pm
Hey Chip
This is the video from the Pres calling NASA. http://www.youtube.com/watch?v=UE1o_1U8cYg


text:
Message board »Message Board home »Sign-in or register to get started
Online now: 7 members: Joe Nap, Patti12, RayTro, STL0, Stacca Nor Cal, jdebose, louurbinati; 81 anonymousDiscussion: Forming women\'s league in Conejo Valley, CA
Posted | Discussion |
Oct. 11, 2004 Next50Years Women's 50 7 posts | Forming women's league in Conejo Valley, CA I am working with the local senior center to start a women's sr. softball league in the Westlake Village area. Plan to start weekly practices and scrimmages in the next few months. Let me know if you want to get on a contact list. |
Oct. 16, 2004 Marianne Women's 50 6 posts | Where is Conejo Valley.......by what major city in Calif.? |
Oct. 18, 2004 Next50Years Women's 50 7 posts | Conejo Valley is the area northwest of LA around Thousand Oaks and Westlake Village. |


text:
AWD / 4WD Vehicles Near Santa Fe, NM
Vehicles equipped with standard or optional 4- or all-wheel drive benefit drivers who live in snowy areas or often use rough roads.
Most Searched AWD / 4WD Models
Near Santa Fe, NMSearch AWD / 4WD Vehicles by Brand
Articles & Reviews
2017 GMC Sierra Denali 2500HD: First Drive Review
2017 GMC Sierra HD pickup arrives with an available all-new Duramax diesel under the hood.
2017 Porsche 911: New Car Review
The 2017 Porsche 911 offers a dizzying array of trims, options and engines, but all are guaranteed to please the most strident enthusiast.
2017 Toyota RAV4: New Car Review
The 2017 Toyota RAV4 doesn't really rise above its top competitors, but its well-rounded range of practical attributes remains appealing.
2017 GMC Sierra 1500: New Car Review
The 2017 GMC Sierra 1500 doesn't have as many unique features as its rivals, but it's nevertheless a dependable, well-rounded workhorse.
2017 Porsche Macan: New Car Review
The 2017 Porsche Macan offers the power and performance of a small sports car with the off-road capabilities of an SUV.
2017 Infiniti Q70: New Car Review
As a flagship sedan, the 2017 Infiniti Q70 must deliver luxury and performance on par with the world's best sedans, and deliver it does.


text:
Friendship Verses
Free online friend verses, friendship poems & friendship quotes for your handmade greeting cards & scrapbooks You are free to use any of our friend verses, |
Your birthday is a perfect time * Your birthday is a perfect time * You are my friend of friends, my confidant, my closest companion * Some of the happiest times of my life * When it comes to having friends * Into every life a little rain must fall... * A friendship is a bond you have with someone else. * God knew what He was doing * I have a list of folks I know... * There's a miracle called Friendship * Everyone hears what you say. * Count your life by smiles, not tears. * A friend is someone who chooses you... * When hands reach out in friendship, hearts are touched with joy. * A friend is someone who understands your past, |


text:
Home..........About..........Online Shop..........Etsy Shop..........Folksy Shop..........Newsletter....
Sunday, December 1, 2013
Win a Mouse!
Back by popular demand! This is your chance to WIN your choice of ornament from The House of Mouse! Simply fill in your email to enter.
a Rafflecopter giveaway
By entering the competition you are agreeing to sign up for The House of Mouse newsletter containing news, updates, exclusive discounts and fun mini interviews with the mice. You can unsubscribe at any time using the link in the newsletter email.
Monday, September 30, 2013
The House of Mouse has reopened!
here!! The mice are overjoyed to be back. Thank you to everyone for
your enthusiasm about their return! The House of Mouse is officially
OPEN! www.thehouseofmouse.etsy.com
Have you ever wanted to own a mouse version of yourself?!? There's a very special giveaway coming later this week, watch this space!
Have you ever wanted to own a mouse version of yourself?!? There's a very special giveaway coming later this week, watch this space!
Sunday, February 17, 2013
Thursday, November 29, 2012
BBC Sherlock and Watson Mouse
This pair of mice are based on Sherlock and Watson from the BBC drama Sherlock. Sherlock mouse has curly black hair, and wears his trademark coat with the collar turned up and his grey scarf. Watson Mouse has dark blond hair, and wears a chequered shirt, cardigan and black coat, in his paws he holds a mobile phone.
These mice were hand sewn using felt, silks, fimo, wire, beads, glue, card and monofilament. They are stuffed with polyfil and stand approximately 2.5inches tall (6.5cm)
Click HERE to see more photos
Tuesday, November 27, 2012
Thursday, November 22, 2012
Black Friday SALE!
This weekend only we are having a SALE for 20% off EVERYTHING! Grab it while you can!
Discount code valid at www.thehouseofmouse.etsy.com and www.thehouseofmouse.co.uk
Friday, November 9, 2012
Christopher Eccleston Doctor Who Mouse
This dark, brooding and vengeful, this regeneration of the doctor only survived for one season. He justifies his northern accent even though he's an alien with "lots of planets have a north". In his trademark dark leather clothes and green shirt he holds his blue topped sonic screwdriver in one paw.
Eccleston Mouse was hand sewn using felt, silks, ribbon, beads, glue, card and monofilament. He is stuffed with polyfil. He stands approximately 2.5inches tall (6.5cm) His costume is not removable.
Click HERE for more photos of him in our shop
Subscribe to:
Posts (Atom)
Popular Posts
A frequently overlooked part of an Etsy store is the policies section. Your store policies tell your customers what to expect from you. I am...
Great photos are one of the most important things to get right if you sell online. Your photographs are your virtual shop window: If they ar...
Back by popular demand! This is your chance to WIN your choice of ornament from The House of Mouse! Simply fill in your email to enter. ...
Email: email@example.com Or fill out the form below:
Earth day is a day designed to inspire awareness and appreciation for the Earth's environment. Something many handmade sellers hold clo...


text:
Definition of divisors
plural of divisor 1
The word "divisors" uses 8 letters: D I I O R S S V.
No direct anagrams for divisors found in this word list.
Adding one letter to divisors does not form any other word in this word list.
Words within divisors not shown as it has more than seven letters.List all words starting with divisors, words containing divisors or words ending with divisors
All words formed from divisors by changing one letter
Other words with the same letter pairs: di iv vi is so or rs
Browse words starting with divisors by next letter
Previous word in list: divisor
Next word in list: divorce
Some random words: rya valgoid veal afeard jnana fever toil
This is not a dictionary, it's a word game wordfinder.
- Help and FAQ - Examples - Home
Privacy and Cookies Policy - Share - © Copyright 2004-2017 Morewords.com - 15.534mS


text:
this wiki
The Halt Psynergy is a Utility Psynergy introduced in Golden Sun. An Adept can obtain it if he or she is equipped with the Halt Gem item, which is found in a treasure chest in Vale Cave. It is of the Jupiter element.
The Halt Psynergy causes a target in a non-battle environment to become immobile in some fashion. If used on a townsperson, that townsperson will flash and when you talk to him he will only say the first word of what he would normally say in that instance, followed by an ellipsis. More practically, it can be used to keep a Djinni keeping its distance away from you shut in one idle position so you can actually reach it (this occurs twice in Golden Sun), and it can cause the automatic statues in the fourth floor of Crossbone Isle to stay put and not block your path. It can also be used to stop certain obstacles in the Colloso competitions by using it on the man with the levers. The Halt Gem and its Psynergy can appear in the inventory of Isaac's party in Golden Sun: The Lost Age if any form of data transfer is used, although it is not needed for any dungeon. Interestingly, this utility can allow the player to "talk" to Djinn in both games that do not need to have Halt cast on; for example, the Venus Djinni found in Vault. This "talking" is really only the portrait, and the text reads "...". This makes it the only time other than when acquiring the Djinni for the first time that these portraits can be viewed.
GBA Utility Psynergy
| |
Golden Sun . | |
The Lost Age
. | |
. | |
Power | |
Phys. Attack | |
Support . | Baffle Card • Bind • Delude • Demon Spear series • Drain • Dull series
• Fresh Breeze series • Impact series • Magic Shell series • Mist • Psy Drain • Sleep • Sleep Card • Ward series • Weaken series |
| |


text:
Automatic registration of multi-modal microscopy images for integrative analysis of prostate tissue sections
© Lippolis et al.; licensee BioMed Central Ltd. 2013
Received: 10 October 2012
Accepted: 29 August 2013
Published: 5 September 2013
Prostate cancer is one of the leading causes of cancer related deaths. For diagnosis, predicting the outcome of the disease, and for assessing potential new biomarkers, pathologists and researchers routinely analyze histological samples. Morphological and molecular information may be integrated by aligning microscopic histological images in a multiplex fashion. This process is usually time-consuming and results in intra- and inter-user variability. The aim of this study is to investigate the feasibility of using modern image analysis methods for automated alignment of microscopic images from differently stained adjacent paraffin sections from prostatic tissue specimens.
Tissue samples, obtained from biopsy or radical prostatectomy, were sectioned and stained with either hematoxylin & eosin (H&E), immunohistochemistry for p63 and AMACR or Time Resolved Fluorescence (TRF) for androgen receptor (AR).
Image pairs were aligned allowing for translation, rotation and scaling. The registration was performed automatically by first detecting landmarks in both images, using the scale invariant image transform (SIFT), followed by the well-known RANSAC protocol for finding point correspondences and finally aligned by Procrustes fit. The Registration results were evaluated using both visual and quantitative criteria as defined in the text.
Three experiments were carried out. First, images of consecutive tissue sections stained with H&E and p63/AMACR were successfully aligned in 85 of 88 cases (96.6%). The failures occurred in 3 out of 13 cores with highly aggressive cancer (Gleason score ≥ 8). Second, TRF and H&E image pairs were aligned correctly in 103 out of 106 cases (97%).
The third experiment considered the alignment of image pairs with the same staining (H&E) coming from a stack of 4 sections. The success rate for alignment dropped from 93.8% in adjacent sections to 22% for sections furthest away.
The proposed method is both reliable and fast and therefore well suited for automatic segmentation and analysis of specific areas of interest, combining morphological information with protein expression data from three consecutive tissue sections. Finally, the performance of the algorithm seems to be largely unaffected by the Gleason grade of the prostate tissue samples examined, at least up to Gleason score 7.
Multiplex analysis Histological sections Hematoxylin & Eosin p63/AMACR Time resolved fluorescence imaging Image registration Scale invariant feature transform Prostate cancerBackground
Prostate cancer (PCa) is the second most common cancer in men worldwide. About 910.000 new cases were recorded in 2008 accompanied with 258.000 deaths. According to current estimates, the incidence of PCa is expected to double by 2030 [1].
Analysis of the microscopic features of the prostate is vital for clinical management of PCa patients, both with respect to diagnosis and prognosis. Today, PCa is commonly diagnosed by a uropathologist carefully examining at least ten transrectal ultrasonography (TRUS)-guided prostate biopsies using conventional brightfield microscopy [2]. Manual morphological analysis is also carried out on whole-mount tissue sections after radical prostatectomy (RP), which may provide valuable prognostic information about outcome of the disease. The most important assessment of the morphology is to determine tumor grade according to the Gleason system [3]. Moreover, considerable research efforts have been directed towards the analysis of tissue sections for assessing the presence of proteins (biomarkers) which can potentially be related to the development and progression of the disease [4]. The study of tissue biomarkers has been expanding since the implementation of Tissue Micro Arrays (TMAs) [5]. Such arrays can contain several hundreds of tissue samples (cores) and have paved the way for high-throughput studies of predictive tissue biomarkers [6].
A common research objective is to investigate the expression of several biomarkers on a stack of consecutive tissue sections. Moreover it is important to be able to recognize specific tissue compartments (benign vs. cancer, epithelial vs. stromal cells, cell cytoplasm vs. nuclei) where such biomarkers are expressed, as this might be related to different states of the disease. There is an unmet need to combine morphological information with protein expression analysis coming from consecutive tissue sections. An automated approach would make this procedure fast and suitable for the study of multiple features on large TMAs.
The aim of our paper is to investigate the feasibility for an integrative analysis through automated registration of digital images of consecutive histological prostate sections stained and visualized with different modalities.
Manual evaluation of histological sections is time-consuming and highly dependent on the user's experience, resulting in high inter- and intra-variability [7]. However the improvement in technology and the access to larger storing facilities in the last decade have led to the creation of digital slide scanners and large digital archives [8]. This paves the way for the use of Image Analysis techniques to handle histological images.
Automated registration of histological sections (stained with the same modality) has been attempted on cervical carcinoma by Braumann et al. [9], while automated registration of multimodal microscopy with application to PCa is considered in a recent paper by Kwak et al. [10]. Their aim was to register pairs of images, from light microscopy and infrared spectroscopy, in order to extract morphological features for use in the classification of cancer versus non-cancer cases. The registration is intensity based, leading to a minimization of a non-convex similarity measure over a four-dimensional space of transformation parameters. This problem is solved using the Nelder-Meade simplex method, which is a local search technique. In contrast, our registration method is landmark-based, with the landmarks coming from Scale Invariant Feature Transform (SIFT), which has the advantage of speed. Moreover, landmark-based methods look for similar features in the image pair rather than dissimilarities and may therefore succeed even in the presence of noise and occlusions. SIFT works with gray-scale images, therefore using more of the original image information when compared to Kwak et al. [10], where only binary (black-white) images were used.
A number of papers explore the possibility to integrate information from in vivo imaging (ex. PET, MRI) with histology [11], and analysis of sequential immunofluorescence staining for assessing several biomarkers [12].
Multiple studies apply SIFT [13] for landmark-based registration of medical images. The earliest of such studies was performed by Chen et al. [14], where unimodal registration was considered. Their experiments are of a very preliminary nature. Other applications are found in Tang et al. [15] and Wei et al. [16]. The former consider alignment of stem cell images whereas the latter is concerned with registration of retinal images, which differs from our problem in that it requires registration transformations of another type (quadric transformations). Another relevant contribution is described by Zhan et al. [17] where texture landmarks, found using scale-space methods, are used in the non-rigid registration (with thin plate splines) of prostate image pairs from histological and MR specimens. For a pair of images, the determination of landmark correspondences and the best registration transformation is found simultaneously by solving a non-linear optimization problem in a large number of variables. Evaluation was carried out for five image pairs.
Tissue acquisition and processing
Tissue samples came from two sources: RP for curative purpose and needle biopsies taken for diagnostic purposes. From the prostatectomy material cores with 1 mm diameter were punched out of relevant blocks and organized in a TMA format. Core needle biopsies are up to 15 mm long and 1 mm wide tissue samples. After the acquisition procedure both types of material were fixed in formalin and embedded in paraffin.
To conduct the study, 4 μm sections were cut from the paraffin blocks and mounted on slides. The pre-processing before staining includes deparaffinization through xylene and ethanol with decreasing concentration, followed by rehydration and antigen retrieval to allow the antibodies to bind to the proteins of interest. The process described above has been performed manually and the accuracy of each step can affect the quality of the final results and introduce artifacts. For example, tissue samples can undergo mechanical deformation during handling and an incorrect preprocessing can cause poor staining and therefore inferior images.
The procedure was done strictly in compliance with the Helsinki Declaration after approval from the Regional Ethical Review Board at Lund University.
In Experiment 1, a TMA containing 88 cores was produced and sectioned. One section was stained for H&E followed by immunohistochemistry for p63/AMACR on the consecutive section. The H&E is a traditional and standardized method in which cellular nuclei are stained with a bluish shade while the cytoplasm is stained with different shades of pink. Slides stained with this procedure are generally used to determine the presence of cancer and assess its aggressiveness. The p63/AMACR is a double staining procedure in which the single basal cell layer surrounding a benign gland has a brown nuclear staining (p63), the cytoplasm in the majority of the cancer cells is stained with reddish shade (AMACR) and the rest of the tissue has different shades of blue. This staining helps the pathologist to spot the presence of cancer or pre-malignant lesions with HGPIN when the histological pattern is inconclusive.
For Experiment 2, sections from biopsies were stained with mouse monoclaonal anti-AR antibody (AR411) which was previously labelled with Europium for TRF. TRF is an evolution of conventional immuno-fluorescence. It uses lanthanide chelates (europium, terbium, etc.) as fluorophores [18]. The long decay times of these isotopes together with a gated acquisition system allow for the detection of a specific signal by excluding the autofluorescence phenomenon, thus obtaining a more linear quantification of the biomarker. Here, TRF is used for the quantification of tissue protein expression in specific compartments as previously shown [19]. After acquisition of images by TRF the AR411 antibody was washed off and the samples were further processed with H&E staining.
Finally, in Experiment 3, one TMA was built containing 50 cores from prostatectomies; four sections were cut, mounted on slides and stained for H&E. This TMA was used to validate results in Experiments 1 and 2 and to study the inner morphological variability of prostatic tissue.
Gleason grading
A normal prostate is organized in glandular structures formed by a layer of basal cells and a layer of epithelial cells surrounding an empty space known as the lumen. Such glands are surrounded by connective tissue called stroma. In presence of cancer, this normal glandular structure is disrupted. The Gleason scoring was introduced in the 1960's and updated in 2005 [20]. It is a system based on histological growth patterns of cancer cells. The Gleason grades (ranging from 1–5) of cancer cells from areas of two distinct growth patterns (two most prevalent) are summed up to form a Gleason score ranging from 2 to 10. A high Gleason grade, and thus Gleason score, is found in less differentiated tumours, that generally are more aggressive and have a poor prognosis [21].
In order to assess the ability of the algorithm to register a large range of images with various morphological characteristics, a pathologist evaluated H&E staining and assigned a Gleason score to each core.
Image acquisition
The Mirax Scan (Carl Zeiss) equipped with Plan-Apochromat 20x/0.75 objective was used to take pictures of H&E and p63/AMACR stained sections.
For Experiment 1, we collected twenty times magnified (20x) images for each core resulting in a total of 88 image pairs (H&E and p63/AMACR in consecutive sections).
For Experiment 2, 106 images pairs (H&E and TRF) were collected. The Nikon Eclipse 600 equipped with an appropriate laser and programmed electronics (Signifer 1432 MicroImager; Perkin-Elmer Life Sciences; Wallac Oy) was used for TRF acquisition. In order to acquire the Europium signal, a filter with excitation and emission bands centered in 340 nm and 615 nm was used. TRF produced forty times maginified (40x) images.
For Experiment 3, we collected 20x images for each core of the four sections.
Image registration
As described in Zitova et al. [22], our registration algorithm pipeline consists of four steps: (1) feature detection and extraction, (2) feature matching, (3) transformation function fitting and (4) image transformation and image resampling.
We first explain the steps (3) and (4), to fix terminology, and then move to SIFT (1) and RANSAC (2).
In our description a gray scale image I is a real valued function I:Ω → [0,1] defined in a planar region Ω, called the image domain, and whose value at a particular point (pixel) x = (x 1, x 2) is the gray level I (x).
Thus T is the combination of a scaling by the factor , a rotation by the angle arctan(b/a) and a translation by (t 1 ,t 2). We define the transformed image T*I 2 : Ω → [0, 1] as the pullback of I 2 by T, that is, by the formula T*I 2(x) = I 2(T(x)) if T(x) ∈ Ω2, otherwise T*I 2(x) = 0. The objective is to find a map T such that T*I 2(x) becomes as similar to I 1 as possible. We do this by finding corresponding keypoints in the two images and then estimate the optimal mapping using Procrustes analysis.
The error becomes ϵ i = y i − B(x i ) z, which is linear in z. (This is possible only in two dimensions).
where ∣∣ ⋅ ∣∣ now denotes the norm in R 2xN . The desired mapping corresponds to the optimal z, which is the solution the normal equations B T B z = B T Y. For this problem to be solvable we need at least two corresponding point pairs. This is sufficient if the pairs are nondegenerate, however, we use at least four point correspondences to get a more well-conditioned problem.
The corresponding keypoint pairs, used in the Procrustes alignment are found using SIFT and RANSAC in a classical manner, described briefly below.
SIFT [23] works by the following principle: first, keypoints are detected in the image. They are local extrema in space and scale when the image is embedded in its scale-space, and they have the property that they are stable under changes in illumination and view-point. Secondly, each such keypoint has a descriptor associated with it, as similar to a fingerprint. In this paper, the keypoint together with its descriptor is called a landmark. The descriptor consists of a 128-dimensional vector containing gradient statistics from eight directions in a 4 × 4 neighbourhood of the keypoint.
This condition is known as Lowe's criterion. It states that the nearest neighbor of the descriptor of x i in the set of descriptors of all the keypoints y j should be much closer than the next-nearest neighbor in order for the keypoint x i to be matched with . We have applied the implementation of SIFT by Vedaldi and Fulkerson [24].
The set of preliminary matches found above may contain a significant percentage of false matches, usually referred to as outliers. The RANSAC algorithm invented by Fischler and Bolles [25] can be used to select a large subset of matches, called inliers, from the set of preliminary matches which is consistent with the registration model. RANSAC is a statistical approach where a small number of preliminary matches are selected at random from the set of preliminary matches and used to estimate a model; in our case we use four preliminary matches to estimate a Procrustes alignment. Using this alignment transformation all keypoints in the first image are transformed into the second image. If a transformed keypoint is within 5 pixels of the keypoint to which it has been matched in the preliminary matching, then the preliminary matching of this pair of keypoints is considered to be an inlier. The number of such inliers is then recorded. This procedure is repeated (in our case 100 repetitions) and the model is chosen which has the highest number of inliers. The final alignment is then estimated by Procrustes analysis using all of the matches in the set of best inliers.
The evaluation procedure
Visual evaluation has the obvious drawback of being subjective, however was chosen in order to save time. Since the human eye is very good at detecting visual inconsistencies we believe that visual evaluation is an appropriate method for evaluating many registrations within a limited amount of time.
We do not, however, rely entirely on visual inspection. In the first of our three experiments we have also performed an extensive quantitative evaluation of the results. Note that the first experiment contains potentially the most challenging of the three registration problems considered in this work since the image pairs consist of adjacent tissue sections stained with different modalities. The quantitative evaluation has two purposes, first of which is to measure the quality of the automatic registration results. Second, the quantitative evaluation was used to show the reliability of the visual evaluation, which was employed in experiments 2 and 3.
With regards to the procedure of the quantitative evaluation, in the 85 of the 88 cases where visual evaluation has classified the automatic registration as correct, the resulting registration transformation is compared to the transformation obtained from Procrustes analysis using manually detected keypoint pairs. More specifically, for each image pair, multiple keypoint pairs were found manually. If the images contained prominent salient features, three to four keypoints were used, otherwise five keypoints were chosen. Procrustes analysis was then performed and the corresponding transformation T manual was recorded.
Note that this is, up to a fixed multiple, the quantity that is minimized in the Procrustes analysis in order to determine the optimal transformation T = T manual .
This is the same expression used in the definition of the intrinsic uncertainty of the manual registration, except that this time the automatically determined transformation T auto is used to map the manually detected keypoints {x i } from the first image into the second image. Note that, since the transformation T manual is defined as the similarity transformation which minimizes the expression then the inequality ϵ manual ≤ ϵ auto is always satisfied.
The tolerance of five pixels corresponds to the tolerance used in the RANSAC sub-procedure of the automatic method. It should also be noted that the average size of a cell nucleus in the images used in our experiments was approximately 5 pixels. This criterion is used to evaluate the performance of the automatic registration method in experiment 1. If the number of quantitatively correct registrations is a large percentage of the images in the sample, then we will conclude that automatic registration is as good as manual registration. Moreover, if the number of quantitatively correct registrations is found to be almost the same as the number of visually correct registrations, then we will conclude that visual evaluation is reliable for our purpose in all three experiments.
Experiment 1
Experiments 1
H&E kyp | p63/AMACR kyp | Initial matches | (Best inliers)/initial matches | Success rate (#correct/#tot) | |
Exp 1 | 953 | 1327 | 67 | 66% | 96.6% (85/88) |
We also analyzed the location of the matching keypoints and found out that 32.6% of them are present within the lumina, 19.4% in the glandular epithelial layer and 48% in mixed areas (between glands).
Experiment 1; performance of the algorithm in different histological classes
Stroma | benign | Gleason 6 | Gleason 7 | Gleason >7 | |
Correct/total | 10/10 | 37/37 | 14/14 | 14/14 | 10/13 |
where the tolerance tol = 5 pixels was used. Using this criterion we found that 82 of the 88 (93.2%) of the image pairs are correctly aligned. Thus, three of the image pairs which were originally considered correctly aligned by the visual evaluation were rejected by the quantitative evaluation. It should be noted that two of these image pairs failed to satisfy the quantitative criterion by as narrow a margin as one fifth of a pixel or less. For comparison, the quantitative criterion was employed with tol = 4 pixels, which gave 80 of 88 (90.9%) correct alignments, and with tol = 6 pixels, which resulted in 84 of 88 (95.5%) correct alignments.
We also computed the statistics of the intrinsic uncertainty of the manual registration and found the mean value μ(ϵ manual ) = 3.38 pixels and standard deviation of σ(ϵ manual ) = 2.60 pixels, hence the estimate ϵ manual = 3.4 ± 2.6 pixels. The corresponding statistics for the automatic registration is ϵ auto = 5.0 ± 3.3 pixels. These estimates should be set in relation to our chosen tolerance tol = 5 pixels.
Experiment 2
Experiments 2
H&E kyp | TRF kyp | Initial matches | (Best inliers)/(initial matches) (%) | Success rate % (#correct/#tot) | |
Exp 2 | 9193 | 672 | 30 | 93% | 97.2% (103/106) |
Experiment 3
In Experiment 3, we performed registration between images of tissue sections, progressively further away from the respective initial section. As explained above, H&E stained sections were used.
Experiment 3
Distance from the reference section | Initial matches | Best inliers | Success rate % |
average (range) | average (range) | (#correct/#tot) | |
consecutive + 1 | 40.9 (6–124) | 25.8 (0–108) | 93.9% (46/49) |
consecutive + 2 | 13.5 (1–33) | 5.6 (0–15) | 52.1% (25/48) |
consecutive + 3 | 10.4 (0–26) | 3.4 (0–12) | 22% (11/50) |
The present study shows that an automated registration of multimodal images from histological prostate sections is possible by using SIFT. Our work is novel with regards to multiple aspects: to our knowledge it is the first time that such algorithm is applied to multimodal microscopy for PCa. Our protocol can detect the presence of multiple antigens.
Pathologists often rely on integration of multiple immunohistochemical stainings (H&E, p63, and AMACR) to perform diagnostics. Experiment 1 shows that in at least 96.6% (visual evaluation) of the cases, the two differently stained sections can be automatically aligned. We therefore present a potential supportive tool for PCa diagnostics since this allows for automated alignment and fast visualization of areas of interest on differently stained sections. In addition, the automated approach displays advantages also for researchers with regards to time efficiency and management of large sample cohorts.
The quantitative evaluation in experiment 1, classified 82 out of 88 (93.2%) image pairs as correctly registered. Thus, the quantitative criterion rejected three image pairs that were originally accepted as correctly aligned by visual evaluation. The two evaluations agree in 96.5% of all cases. Of note, two of these three images failed to meet the quantitative criterion by a margin as small as one fifth of a pixel. If we accept also these images pairs as correctly aligned, then the agreement goes up to 98.8%. Based on these results we considered visual evaluation as a reliable way of assessing automated registration in all experiments.
In our protocol we have not only used different immunohistochemical staining but have also made use of TRF. TRF allows for biomarker quantification due to its signal linearity. As a result, we are able to integrate morphological information from H&E with quantitative analysis of the expression of a certain molecule (in this case AR). Biological studies on the AR status in prostate tissues using our method are ongoing. In the TRF and H&E registration we observed a success rate of 97% (103 out of 106 images). In addition, the performance of the algorithm seems to be very stable as displayed in the observation that 93% of the initial matches were identified as best inliers. Robustness to obstruction was also confirmed through correct alignment of corrupted images (Figure 4). However, the fact that we re-stained the same tissue section following removal of the antibody may have contributed to the good performance. In order to do the re-staining, we performed an optimization of the protocol. Nevertheless, these data show that reusing the same tissue again for re-staining does not affect the quality of the resulting images and the registration process.
In our third experiment we tested automated alignment of several consecutive H&E tissue section (4 in our initial setting). In our mind this would have given us the upper limit of sections that could be aligned and analysed for the expression of several biomarkers in the same area of interest. We observed that sections further than one section away from each other have already some substantial differences in the structure (assessed by an independent observer), which can explain the lower success rate in automated alignment. With this in consideration, the use of more than 3 consecutive sections for multiple staining analysis will not guarantee overlap of the area of interest. In order to address this issue, one could consider optimizing protocols for multilabeling of the same tissue section. In this regards, immunofluorescence would technically be the best solution for quantification.
Since PCa is a very heterogeneous disease we have assessed the performance of the algorithm for samples with different Gleason scores. The algorithm correctly aligned all the images with Gleason equal to or lower than 7, which is the most common Gleason score detected in patients. For patients with higher Gleason scores, 10 out of 13 images were correctly aligned. This may be due to the fact that the higher Gleason scores are characterized by a more complex structure. They present with fused glands and scattered individual cancer cells resulting in a highly variable appearance across consecutive sections.
Due to the fact that the image pairs in question have different modalities and may therefore not be easily aligned by minimizing an intensity-based dissimilarity measure, we have chosen a landmark-based registration method for the image alignment. An intensity-based dissimilarity measure was used in Kwak et al. [10] for images of different modalities; however this required first a transformation into binary images. In our method we use grayscale images in order to retain more of the available image information. Moreover, landmark-based methods focus on features two images have in common and ignore dissimilarities. In addition, landmark-based registration is also easier to compute and therefore a potentially faster method.
In our work the landmarks were extracted from the images by first detecting keypoints and descriptors using SIFT. True correspondences between keypoints were subsequently established using the descriptors and RANSAC. Both SIFT and RANSAC are well-established tools in computer vision and image analysis. The proposed method has been used for other medical registration problems, but the application to PCa and to these specific modalities (whose advantages have been explained before) is, to our knowledge, novel.
The time it takes to transform one image was used as a unit to measure the computational performance of the proposed algorithm. This is an operation fundamental to all registration problems and therefore appropriate for comparisons. We observed that 6% of the total time it takes to register two images (typically 1000×1000 pixels each) is used for the image transformation. In addition when analysing the performance in detail we found that the bottleneck of the algorithm is the computation of distance matrix, which is used to compare the keypoint descriptors derived from SIFT. This computation represents 40% of the total time required by the algorithm, which is nearly seven times the amount required for one image transformation. There are no algorithms able to find the exact nearest neighbor in a more efficient way than exhaustive search, however the Best Bin First [26] can speed up the computation by finding it with a certain probability. The average runtime of our script was 7.6 seconds per image pair, including visualizations, using a Matlab implementation. We have observed that a preprocessing step, which deletes all the spurious background keypoints, can reduce the distance matrix to 50% of its original size. Unfortunately, we have not been able to obtain information about the performance of the registration method described by Kwak et al. [10] However, we can infer from the method that they use that computation of the intensity-based dissimilarity measure requires one image transformation. Their optimization method (in four-dimensional space) requires five such computations just to get started and a number of iterations in order to converge to a good solution. Unless their method converges in about ten iterations, it cannot possibly be faster than the one proposed by us.
One must however mention that the current study may have some limitations. The work is a proof of principle study and therefore is performed on a limited number of samples. In addition, the samples come from one single institution and therefore the results must be validated by further studies conducted at several independent institutions.
In this study we have investigated the potential to automatically align microscopic images of prostate tissue sections stained with different modalities. This addresses the need for integration of morphological information with protein expression data allowing for a more detailed description of PCa. Our results, based on the use of SIFT algorithm shows that potentially 3 consecutive sections of prostate tissue with different stainings can be aligned in an unsupervised way allowing for successive analysis of the tissue. Of note, good results were obtained when aligning H&E and p63/AMACR images (96.6% of images correctly aligned using visual evaluation) and even better results were obtained when aligning TRF and H&E images (97%). This shows that the algorithm performed well also with less informative images such as 1-channel TRF (it must be said that in this case using the same section for producing the 2 images might have contributed to the very high success rate). The advantage in terms of time efficiency is very clear when considering that typical research studies can include thousands of tissue samples and therefore thousands of comparisons that otherwise must be performed manually. The results in experiment 3 confirm what was observed in the other experiments and suggest that the number of easily alignable consecutive sections may be limited to 3. Therefore, if one wishes to investigate many biomarkers, it is preferable to develop multi-staining procedures to be performed on the same slide. Currently work in the field of clinically relevant image analysis remains limited. Our study is therefore a novel approach that supports implementation of automated image analysis in the field of PCa diagnostics and prognostics.
Alpha-methylacyl-CoA racemase
Hematoxylin and eosin
Time resolved fluorescence
Scale invariant feature transform
- PCa:
Prostate cancer
Transrectal ultrasonography
Radical prostatectomy
Tissue microarray
High grade prostate intraepithelial neoplasia
Androgen receptor.
The authors wish to thank Elise Nilson for doing all the tissue sections staining, Prof Kim Pettersson and Mari Peltola at the department of Biotechnology at Turku University for labeling monoclonal IgG mAB against androgen receptor with europium and Olof Enqvist at the Centre for Mathematical Sciences at Lunds University for fruitful discussions. This work was financially supported by European Union 7 Framework/Marie Curie Initial Training Networks (ITN) PRONEST/FP7-PEOPLE Contract no. 238278, and FAST-PATH/FP7-PEOPLE Contract no. 285910, the Swedish Cancer Foundation, the Swedish Research Council, Governmental funding of clinical research within the National Health Services ("ALF" grant), University Hospital Research Foundations, European Research Council (grant no. 209480, GlobalVision) and the Gunnar Nilsson's Cancer Foundation.
Authors' Affiliations
- Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D: Global cancer statistics. CA Cancer J Clin. 2011, 61 (2): 69-90. 10.3322/caac.20107.View ArticlePubMedGoogle Scholar
- Heidenreich A, Aus G, Bolla M, Joniau S, Matveev VB, Schmid HP, Zattoni F: EAU guidelines on prostate cancer. Eur Urol. 2008, 53 (1): 68-80. 10.1016/j.eururo.2007.09.002.View ArticlePubMedGoogle Scholar
- Gleason DF: Classification of prostatic carcinomas. Cancer Chemother Rep. 1966, 50 (3): 125-128.PubMedGoogle Scholar
- Bjartell A, Montironi R, Berney DM, Egevad L: Tumour markers in prostate cancer II: diagnostic and prognostic cellular biomarkers. Acta Oncol. 2011, 50 (Suppl 1): 76-84.View ArticlePubMedGoogle Scholar
- Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med. 1998, 4 (7): 844-847. 10.1038/nm0798-844.View ArticlePubMedGoogle Scholar
- Bubendorf L, Nocito A, Moch H, Sauter G: Tissue microarray (TMA) technology: miniaturized pathology archives for high-throughput in situ studies. J Pathol. 2001, 195 (1): 72-79. 10.1002/path.893.View ArticlePubMedGoogle Scholar
- He L, Long LR, Antani S, Thoma GR: Histology image analysis for carcinoma detection and grading. Comput Methods Programs Biomed. 2012, 107 (3): 538-556. 10.1016/j.cmpb.2011.12.007.View ArticlePubMedPubMed CentralGoogle Scholar
- Ho J, Parwani AV, Jukic DM, Yagi Y, Anthony L, Gilbertson JR: Use of whole slide imaging in surgical pathology quality assurance: design and pilot validation studies. Hum Pathol. 2006, 37 (3): 322-331. 10.1016/j.humpath.2005.11.005.View ArticlePubMedGoogle Scholar
- Braumann UD, Kuska JP, Einenkel J, Horn LC, Loffler M, Hockel M: Three-dimensional reconstruction and quantification of cervical carcinoma invasion fronts from histological serial sections. IEEE Transactions on Medical Imaging. 2005, 24 (10): 1286-1307.View ArticlePubMedGoogle Scholar
- Kwak JT, Hewitt SM, Sinha S, Bhargava R: Multimodal microscopy for automated histologic analysis of prostate cancer. BMC Cancer. 2011, 11: 62-10.1186/1471-2407-11-62.View ArticlePubMedPubMed CentralGoogle Scholar
- Park H, Piert MR, Khan A, Shah R, Hussain H, Siddiqui J, Chenevert TL, Meyer CR: Registration methodology for histological sections and in vivo imaging of human prostate. Acad Radiol. 2008, 15 (8): 1027-1039. 10.1016/j.acra.2008.01.022.View ArticlePubMedPubMed CentralGoogle Scholar
- Wahlby C, Erlandsson F, Bengtsson E, Zetterberg A: Sequential immunofluorescence staining and image analysis for detection of large numbers of antigens in individual cell nuclei. Cytometry. 2002, 47 (1): 32-41. 10.1002/cyto.10026.View ArticlePubMedGoogle Scholar
- Lowe DG: Object Recognition from Local Scale-Invariant Features. Proceedings of the Seventh IEEE International Conference on Computer Vision, 1999, Volume 2. 1999, Kerkyra, Greece: IEEE Computer Society, 1150-1157.View ArticleGoogle Scholar
- Chen J, Tian J: Rapid multi-modality preregistration based on SIFT descriptor. Conf Proc IEEE Eng Med Biol Soc: 30 Aug-3 Sep 2006; New York, USA. 2006, Piscataway, NJ: IEEE Service Center, USA, 1437-1440.View ArticleGoogle Scholar
- Tang CM, Dong Y, Su XH: Automatic Registration based on Improved SIFT for Medical Microscopic Sequence Images. Proceedings of 2008 International Symposium on Intelligent Information Technology Application: 20-22 Dec 2008, Volume 1. 2008, Shanghai, China: IEEE Computer Society, 580-583.View ArticleGoogle Scholar
- Wei LF, Pan L, Lin L, Yu L: The Retinal Image Registration Based on Scale Invariant Feature. 3rd International Conference on Biomedical Engineering and Informatics (Bmei 2010): 16-18 Oct 2010, Volume 2. 2010, Yantai, China: IEEE, 639-643.View ArticleGoogle Scholar
- Zhan Y, Feldman M, Tomaszeweski J, Davatzikos C, Shen D: Registering histological and MR images of prostate for image-based cancer detection. Med Image Comput Comput Assist Interv. 2006, 9 (Pt 2): 620-628.PubMedGoogle Scholar
- Seveus L, Vaisala M, Syrjanen S, Sandberg M, Kuusisto A, Harju R, Salo J, Hemmila I, Kojola H, Soini E: Time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization. Cytometry. 1992, 13 (4): 329-338. 10.1002/cyto.990130402.View ArticlePubMedGoogle Scholar
- Siivola P, Pettersson K, Piironen T, Lovgren T, Lilja H, Bjartell A: Time-resolved fluorescence imaging for specific and quantitative immunodetection of human kallikrein 2 and prostate-specific antigen in prostatic tissue sections. Urology. 2000, 56 (4): 682-688. 10.1016/S0090-4295(00)00671-3.View ArticlePubMedGoogle Scholar
- Epstein JI, Allsbrook WC, Amin MB, Egevad LL: The 2005 international society of urological pathology (ISUP) consensus conference on Gleason grading of prostatic carcinoma. Am J Surg Pathol. 2005, 29 (9): 1228-1242. 10.1097/01.pas.0000173646.99337.b1.View ArticlePubMedGoogle Scholar
- Mellinger GT, Gleason D, Bailar J: The histology and prognosis of prostatic cancer. J Urol. 1967, 97 (2): 331-337.PubMedGoogle Scholar
- Zitova B, Flusser J: Image registration methods: a survey. Image and Vision Computing. 2003, 21 (11): 977-1000. 10.1016/S0262-8856(03)00137-9.View ArticleGoogle Scholar
- Lowe DG: Distinctive image features from scale-invariant keypoints. International Journal of Computer Vision. 2004, 60 (2): 91-110.View ArticleGoogle Scholar
- VLFeat: An open and portable library of computer vision algorithms. http://www.vlfeat.org/,
- Fischler MA, Bolles RC: Random sample consensus - a paradigm for model-fitting with applications to image-analysis and automated cartography. Communications of the Acm. 1981, 24 (6): 381-395. 10.1145/358669.358692.View ArticleGoogle Scholar
- Beis JS, Lowe DG: Shape indexing using approximate nearest-neighbour search in high-dimensional spaces. 1997 IEEE Computer Society Conference on Computer Vision and Pattern Recognition, Proceedings: 17-19 Jun 1997. 1997, San Juan, Puerto Rico: IEEE Computer Society, 1000-1006.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/13/408/prepub
Pre-publication history
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
Welcome! Drawing from a creative pool of more than 40 years, you will find here how-to tips galore! Lots of quotes and tidbits to make you smile and to make your crafting/baking easier. Favorite recipes, websites, paper dolls and more. Many vintage images + to enjoy! An eclectic swirl of fun crafting and art!
Friday, May 25, 2012
Brunei small country with a long history
Those are some of the ways to say 'Peace' in the country of Brunei!
The first is Malaysian for Peace:
The second is in the Bisaya language for peace:
The third and fourth are in different dialects of Chinese for peace:
Why this interest in the country of Brunei?
Well, I started to get readers from there.
I said to myself -
I never heard of that country!!!
Is that really a country?
Yep, it is.
Check out the map above. You can see it is a tiny country,
actually smaller than our state of Delaware!
and that's a small state...
Brunei Flag
So, what are the people of Brunei like?
Here are some quick and fun facts:
Last estimate was in July, 2012
and that was estimated at about 409,000 people.
I'm pretty sure Denver is bigger than that!
The Bruneian people are from a wide diversity of national backgrounds.
There is about 15% Chinese,
about 18% indigenous and other
(don't you always want to know what 'other' means?!!!)
and just a reminder, indigenous means 'native peoples',
not emigrated from somewhere else.
There is a sizable Indian population, mostly minorities from Southern India.
Just in that section some of the different languages are
Think we're done yet?
Just in the Chinese sector there is
and Hainanese
button with official coat of arms heraldry of Brunei
there are expatriate communities that include Filipino and Indonesian...
How do they ever communicate with each other?
(I'm thinking Tower of Babel from the Bible.....)
Well, English is widely used in business and the work force.
If you are in High School, or on to College,
all the instructions are in English!
the official language of Brunei is Melayu Brunei,
a form of Malaysian, but different.
Of course.
Melayu Brunei was made official in 1959
with the signing of the Brunei 1959 Constitution.
Brunei is a
constitutional sultanate
yep, I never heard of that either!
This is a photo taken at the wedding of Princess Hajah Majeedah Nurul Bulqiah,
daughter of the first wife Kebawah Duli Anak Hajah Saleha
binti His Majesty Sultan Haji Hassanal Bolkiah Mu'zzaddin Waddaulah
Yang Amat Mulia Pengiran Anak Khairul Khalil
bin Pengiran Syed Haji Jaafari
September, 2008
(I bet they hated learning how to spell their own names in grade school!)
As near as I can tell binti means daughter of?
so bin would be son of?
Royal Wedding
I wanted to find more information on all that wonderful beading,
but never did find any.
boo hoo
Another fun fact:
The average age of Bruneians is
27 years old!
and the life expectancy is averaged at
male: 71 and female: 76
photo cred: Anthony Asael
Brunei History
now don't skip this just because you hate history!
It really can be quite fascinating!
Brunei is one of the oldest kingdoms in the world
as well as one of the youngest independent nations in Southeast Asia!
In 1888 Brunei became a British protectorate
and independence was achieved in 1982.
the same family has ruled Brunei for over 6 centuries.
stone boat in lagoon
Brunei Bandar Sei Begawan Omar Ali Saifudden Mosque
The official name is
State of Brunei Darussalam
the capital is
Bandar Seri Begawan
The last election was held in 1962, and the next election is NA.
The Sultanate is handed down through the family.
Sultan Hassanal Boliah was coronated in 1968.
Oil was discovered in 1929.
Some of the cars found in the Sultan of Brunei's Garage.
It holds over 7,000 vehicles worth over 5 billion $$.
What kind of religion do they practice?
woman of Brunei
The official religion is Muslim with 67% of the population practicing.
This is reflected in their legal system which is based on
English common law;
for Muslims, Islamic Shari'a law supersedes civil law in a number of areas.
About 13% are Buddhist,
10% have indigenous beliefs, and that mysterious 'other',
and about 10% are Christian.
According to www.worldwatchlist.us
there are approx. 39,500 Christians.
In 2011 monitoring of Christian meetings increased.
Only non-Malays can choose any religion other than Islam.
If a Malay converts they are scheduled for
're-education into Islam'
and charged with
'Disturbs peace and harmony'.
There are restrictions on importing Christian materials, Bibles and literature
making them difficult to obtain.
Brunei forests of Temburong
Brunei culture draws from the Old Malay World,
including the Malay Archipelago
and stemmed from the Malay Civilization.
Influences include animism, Hinduism, Islam and the West.
traditional clothing of Brunei
Misc. Fun Facts:
A large portion of the labor force are from
and Bangladesh.
Water taxis are a common form of transportation.
The country has made a serious effort in encouraging native crafts,
including the traditional arts of
boat making
silver smithing
bronze tooling
cloth weaving
mat and basket weaving
wood carving and wood working.
traditional silver work
basket weaving at a raft Show in the Brunei Darussalam Pavilion
Silver work, Brass ware and wood working were already a flourishing business
by the 15th century!!
Well, I hope you have enjoyed this very brief armchair overview of
map of Brunei, Malaysia and the South China Sea
A last little fun fact:
Korea established diplomatic relations right after the 1982 independence of Brunei from the United Kingdom.
Many Bruneians have become increasingly attracted to Korean
popular soap operas
and songs!
The capital of Brunei, northern bank, Bandar Sei Begawan
It looks like it has many beautiful places.
Shall we go?
I gleaned information from many different sites.
Here are most of them:
www.yynr.org - an article by Safarozi
www.koreatimes.co.kr - an article written by Na Jeong-ju
until next time
3 comments:
I lived in bsb, Brunei in early 80's during the construction of the Sultan's new istana (Palace). Brunei actually gained it's independence in 1982. All foreigners had to leave Brunei for a week while they celebrated their independence.
This country is so beautiful and amazing and I'll never forget the friends I made and the experiences I had and at first opportunityI'll be going back!
Subscribe to:
Post Comments (Atom)


text:
Sacramento State main news page news releases news and events archive facts & stats experts guide image downloads news by e-mail contact news services staff submit news & events feedback Sac State Magazine Sacramento State Bulletin Newslink Sacramento State Catalog events this month search events academic athletics commencement Capital Public Radio alumni association crime alerts construction CSU Newsline visitor relations commencement Sacramento State ticket office campus directory campus tours
April 5, 2006
Officials to face questions from students on teens, tougher driving laws on Thursday, April 6
Ten top high school students from across California will become reporters for a day as they conduct a press conference with state officials on teens and tougher driving laws on Thursday, April 6 at 11 a.m. in room 126 in the Capitol. The press conference is part of the day-long Student Legislative Summit on teens and driving sponsored by the LegiSchool Project, a civic education project between the Center for California Studies at Sacramento State and the State Legislature. The event is designed to familiarize students with the legislative process and to offer a forum for young people to share their thoughts and concerns on public policies that may impact them. Students will question Assemblyman John Benoit (R-Palm Desert), a former California Highway Patrol commander who sits on the Assembly Budget Subcommittee on Transportation, and Paula LaBrie, who is legislative counsel on government affairs for the California State Auto Association. Sacramento State Professor Paula Gardner will serve as moderator. More than 100 high school classes are expected to view the press conference on the California Channel or on videotape. The students were selected from LegiSchool's recent statewide essay contest on "Teens and Driving: Changing the Rules of the Road," in which high school students were asked about cell phone use by teens while driving, a new law that limits late night driving by teens and whether teen drivers are being adequately trained behind the wheel. The essay contest winners include Sana Ahmed, Upland High; Jasdeep Brar, Fowler High; Samuel Chiu, Upland High; Christina Hinojosa, Adelante High, Roseville; Ellen Hou, Arcadia High; Vanessa Johnson, Madera High; Allison Jones, Upland High; Grace McCullough, Los Angeles County High School for the Arts; Jennifer Pham, Middle College High, San Jose; and Rachel Warner, Los Angeles County High School for the Arts. For more information, call the Center for California Studies at (916) 278-6906 or the Sacramento State office of public affairs at (916) 278-6156.
California State University, Sacramento Public Affairs 6000 J Street Sacramento, CA 95819-6026 (916) 278-6156 email@example.com


text: The Mio A201 has been created with flexibility in mind. It's a pocket-sized satellite navigation system that allows you to listen to music, play games, store digital photos and carry your Outlook Contacts and Calendar.It's a PDA, a GPS system, an MP3 player, a photo viewer...A perfect journey, every timeInstall whichever GPS software you prefer and let the Mio A201 guide you to your destination.Pocket-sized portabilityTechnically impressive on the inside, the Mio A201 doesn't disappoint on the outside either.Packed full of featuresAs a pocket PC the Mio A201 synchronises with Outlook on your desktop, so your calendar and contacts can go with you.A perfect journey, every timeThe next-generation SiRF Star III chipset and rotational antenna not only pick up satellite signals faster and hold them better, but also ensure optimal reception in both landscape and portrait positions.And you can connect a Traffic Message Channel (TMC)* antenna** to receive up-to-the-minute road information wherever you travel in Europe*.The none-reflective, smudge-free screen looks clear in broad daylight and in the dark.Pocket-sized portabilityThe elegant design fits perfectly into your hand, yet it's tough and well built at the same time.Enjoy pocket-sized portability you won't want to leave behind, whatever you're doing. Use it to find your way walking around city centres (and it will always remember where you've parked).Download a route and take it mountain biking or hill walking. Whenever you need to know where you're going, whether you're on a boat, in a plane, on a motorbike... the Mio A201 makes sure you get there.But satellite navigation is just the start.Packed full of featuresPocket Word and Excel for Windows Mobile 5.0 mean you can keep up with work (if you really want to). And you can also use it to listen to music, play games and store digital photos.The Mio A201 also comes with built-in Bluetooth connectivity, so you can enjoy a world without wires. Wireless connection with Bluetooth-enabled computers, PDAs and phones makes it easy to exchange files, music and photos.With the Mio A201, you stay connected wherever you are.Hands-free phone functionConnect your Mio A201 wirelessly with a Bluetooth-enabled mobile phone and you have a ready-to-go hands-free kit!Extra softwareThe Mio A201 really is a very convenient tool for your every need thanks to the pre-installed additional software.eConvertorWant to know how much euro $100 stands for?How many yards in a meter?These conversions (or any other calculation) are now a piece of cake thanks to the e-Convertor!BT DialerWireless connection with Bluetooth-enabled computers, PDAs and phones makes it easy to exchange files, music and photos.Picture viewerPicture viewer allows you to preview your digital photos on a larger screen and to profit from the extra storage capacity so you can save memory space on your camera.eUtilityWith this highly convenient tool you can manage a set of extra settings: system information, performance management, power settings and USB specifications for ActiveSync.


text:
Connecticut Retreat: a magical hide-away
From $150 / night
Description from the owner
Description from the owner
Bedrooms: 2
/Bathrooms: 1
/Sleeps: 6
/Type: House
Book this 2 bedroom, 1 bathroom house in Chester today! A total of 6 people can sleep here comfortably.
Guests can enjoy a deck, outdoor grill, and air conditioning among other amenities offered by this rental.
Travelers are sharing their opinions on this property. It has an overall rating of 5/5 thanks to an average of 8 reviews.
Costs vary, with the average weekly rate as low as $994.00, depending on travel dates.
The owner responds to 50% of inquiries, which is lower than average, and last updated the property's calendar 2 weeks ago.
Proximity to TripAdvisor's Top Chester Attractions
Proximity to TripAdvisor's Top Chester Attractions
Reviews (3)
Also consider
- FlipKey has rentals in over 11,000 destinations around the world:


text:
How do we communicate the "Good News" of Jesus Christ to those around us?
The Orthodox Conference on Missions and Evangelism
January 20-22, 2012 Dallas and Ft. Worth
Friday January 20, 2012 at St. Barbara Orthodox Church (OCA), Ft. Worth
Saturday, January 21, 2012 at Holy Trinity Greek Orthodox Church, Dallas
Sunday, January 22, 2012 at various orthodox parishes
This Conference will examine how the Church of the past evangelized the world,
and discuss how to put ancient proven methods into modern practice locally.
Fr. Peter Gillquist Chair of the Antiochian Archdiocese Department of Missions and Evangelism |
Fr. Joseph Huneycutt St. Joseph Orthodox Church Houston, TX |
Fr. Evan Armatas St. Spyridon Orthodox Church Loveland, CO |
For the latest information and to register please go to www.ntom.org
St. Barbara Orthodox Church (OCA)
5201 Altamesa Boulevard
Fort Worth, TX 76133-5927
(817) 294-0325
Holy Trinity Greek Orthodox Church
13555 Hillcrest Road,
Dallas, TX 75240-5412
(972) 991-1166
A joint program of the Antiochian Orthodox Christian Archdiocese Department of Missions and Evangelism, www.antiochian.org/missio... [2] ,
and North Texas Orthodox Missions
Free admission – No childcare provided
Attachment | Size |
me_conference_posterrevised.pdf [1] | 202.68 KB |


text:
Housekeeping Jobs including kitchen in Norwalk, Connecticut
1 Norwalk Housekeeping Jobs Found
Kathy Green
Post a ListingNot finding what you're looking for?
Why not create a listing and let them come to you?
Find or become a housekeeper.
Have an account? Log In
single, young and BUSY professional seeking a housekeeper to come in once weekly or biweekly to help with household chores. I am looking for someone honest, efficient, and reliable.... Read More
Why not create a listing and let them come to you?
Top Cities for Housekeeper.com | |||||||||||
Housekeeper.com Services


text:
Brad Andrew Williams is an Australian cricketer.
He is a right arm fast bowler who made his first-class debut for Victoria in the 1994–95 season as a nineteen year old. At the time he had raw speed and was widely tipped as a future star for the national team , although his debut for Australia didn't come until a One Day International match against New Zealand in January 2001.
More on Wikipedia…
(16 quotes found)
"There was no splatter or droplets of blood which can happen with gunshot wounds,"
Brad Williams
"We support encryption,"
"There is no quick fix to digital piracy."
"They have to embrace digital media technology and the Internet, instead of subverting it with regulation."
"We support encryption. But there is no magic fix for music piracy."
"If there's any place that Bode connects, it's with kids. The way CBS portrayed him in their press release gave kids this misguided view that this is how they should be acting."
"The slowdown in PC sales has been pretty well documented, and we're trying to give consumers a reason to buy,"
"We believe it will be hard to accumulate enough reserves to fully insulate the state from a spending reduction,"
"They chose 10 riders in the nationals to watch, and will pick five."
"We believe it will be hard to accumulate enough reserves to fully insulate the state from a spending reduction."


text:
have you visited? Keep track at VT251.com. |
Check out VermontJobsSite.com. |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
About Vernon, Vermont
Windham County
*Area, Population and Density rankings refer to Vernon's relative position among Vermont's 255 civic entities (9 cities, 242 towns, 4 gores and grants). Complete rankings are here.
Vernon has one of the most involved histories of any town in Vermont. Originally the area was included in the Massachusetts grant for Northfield, which was made in 1672, and some Vernon land titles actually derive from that grant. The area was later part of the Equivalent Lands (given by Massachusetts in exchange for land that colony had mistakenly sold). Later still, in 1736, Massachusetts granted Fall Town (sometimes Fallstown), and that grant also covered part of Vernon. In 1753 New Hampshire had granted Hinsdale, on both sides of the Connecticut River, including part of modern Vernon. When the river was established as the boundary between New Hampshire and Vermont, there were established separate but neighboring towns in separate states, both named Hinsdale. By the beginning of the 19th Century, the people of Hinsdale, Vermont were tired of having the same name as their neighbors across the river, and in October, 1802 the Vermont legislature changed the town's name to Vernon.
Soon after Massachusetts made the 1736 grant, two forts were built by men who had received lands under that grant. Josiah Sartwell built his fortified residence within the present town of Vernon. Fort Sartwell, as it became known, was the site of several Indian raids and attacks. During one of the raids, Sartwell's daughter, Jemima Sartwell Phipps Howe, was taken captive, along with her seven children, and Howe was killed. She eventually was returned home and lived to marry again, her last husband,being Amos Tute, who died in 1790. The epitaph on his gravestone has often been cited as one of the most high-minded in the area:
Were I so Tall to Reach the Pole Fort Sartwell stood until 1837, at which time it was torn down and some of the timbers were used in a new building.
About the same time that Josiah Sartwell built his fort, Orlando Bridgman built another fortified home, also in Vernon. However, his fort fared less well than Sartwell's. Fort Bridgman was burned and rebuilt in 1747, and then it was captured and destroyed for the last time in 1755.
When New Hampshire granted an area on both sides of the Connecticut River in 1753, it named the town for one of the valley's most illustrious pioneers: Ebenezer Hinsdale came from a Deerfield, Massachusetts family. His mother was one of the people taken captive in the famed Deerfield Raid in 1704. Hinsdale graduated from Harvard and later was ordained in Boston's Old South Church in order to become a missionary to the Indians in the Connecticut valley; he was also chaplain of Fort Dummer, then the biggest trading post in the area. Very shortly, he became a colonel in the army and a trader instead of a missionary. He subsequently received land in many different New Hampshire grants, including Hinsdale.
According to a story told in Hemenway's Gazetteer, Vernon got its name through the intervention of a woman. The townspeople had decided that they wanted the name changed to Huntstown after Arad Hunt, one of the town's most prominent citizens: justice of the peace for many years, the town's first representative to the Legislature, and earlier a member of the Council of Safety. As the story (perhaps apocryphal) goes, the townspeople charged their town representative to ask the legislature for the Huntstown name, but the legislator's wife thought that name was too ordinary and told him to ask for Vernon instead. That he did, and the lthe town was renamed Vernon in October 1802. It is thought that the wife had Mount Vernon (George Washington's home) in mind; Washington had died in 1799 and was buried in a tomb on that estate (named by Washington's half-brother, Lawrence, who had served under Admiral Edward Vernon, the British naval hero).
Material excerpted or adapted from Esther Munroe Swift's
Activities & Points of Interest Goings-on in and near Vernon | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Contact Info Emergency Services (Statewide): 911
Churches, Ministries, Charitables Seventh Day Adventist : Mountain View 802-254-5088 |
Schools Windham Southeast Supervisory Union 802-254-3731 |
Neighboring Towns | ||||||||||||||||||||||||||||||||||||||||||||||||||||
This is a basic geographic reference, intended to show relative location of adjacent towns. Directional accuracy is limited to 16 compass points. There isn't even the slightest suggestion that one can necessarily travel directly from one town to the next (as in "You can't get there from here").
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
| Businesses & Services
| Utilities Notes about utilities:


text:
Welcome to the BIOMARGIN website
Download |
Whether you are a patient, a scientist, a company or a foundation involved in renal transplantation, we hope that you can find here all information you need about our European Commission - supported programme "BIOMARGIN", aiming at discovering and validating robust non-invasive biomarkers for the follow up of renal grafts.
In renal allograft recipients, 10-year graft survival has not improved over the past decades. Histological examination of graft biopsies has long been the gold standard to confirm graft injuries, but biopsies are invasive and histological grading is not very robust. There is thus a need for robust, non-invasive methods to predict and diagnose acute and chronic graft lesions, to improve patient treatment, quality of life and long-term graft survival.
Also, there is an unmet need for better understanding of the immune and non-immune mechanisms of interstitial fibrosis /tubular atrophy and graft loss.
Combining all the skills required to build upon previous findings, BIOMARGIN will offer such opportunities in renal transplantation by integrating several omics approaches (mRNA, miRNA, peptides, proteins, lipids and metabolites) in blood, graft tissue and urine, in a well thought out, multistage discovery-to-validation translational programme, following the highest European ethics and regulatory requirements, as well as quality controls and quality assessments at all clinical and analytical steps.
BioMargin International Workshop
Methodological aspects of biomarker discovery and validation: BIOMARGIN as a case study
BioPark Paris 2017 Feb. 21st,
Information and Registration Here
Mark your agendas! BioMargin International Workshop in Paris, France February 21st 2017. More information Here
You can read about recent results in the BioMargin Project in the last Publishable Summary(Pdf)


text:
Give us a call to get our secret deals 1-800-468-3578
Property Location
With a stay at Hotel Nine, you'll be centrally located in Phnom Penh, steps from Preah Sihanouk Garden and minutes from Cambodia-Vietnam Friendship Monument. This family-friendly hotel is close to Royal Palace and Tuol Sleng Genocide Museum.
Make yourself at home in one of the 15 individually decorated guestrooms, featuring minibars and DVD players. Rooms have private furnished balconies or patios. LCD televisions with cable programming provide entertainment, while complimentary wireless Internet access keeps you connected. Private bathrooms with showers feature rainfall showerheads and complimentary toiletries.
Rec, Spa, Premium Amenities
Take time to pamper yourself with a visit to the full-service spa. After enjoying recreational amenities such as an outdoor pool, a lucky night at the casino is the perfect end to the day. This hotel also features complimentary wireless Internet access, concierge services, and babysitting/childcare (surcharge). Guests can catch a ride to nearby destinations on the area shuttle (surcharge).
Satisfy your appetite at the hotel's restaurant, which features a bar and a pool view. Dining is also available at a coffee shop/café. Relax with your favorite drink at a bar/lounge or a poolside bar. A complimentary cooked-to-order breakfast is served daily.
Business, Other Amenities
Featured amenities include complimentary high-speed (wired) Internet access, a 24-hour business center, and a computer station. A roundtrip airport shuttle is provided for a surcharge (available on request), and free self parking is available onsite.
You must present a photo ID when checking in. Your credit card is charged at the time you book. Bed type and smoking preferences are not guaranteed.Your reservation is prepaid and is guaranteed for late arrival. The total charge includes all room charges and taxes, as well as fees for access and booking. Any incidental charges such as parking, phone calls, and room service will be handled directly between you and the property.


text:
Curls Curlicious Curls Cleansing Cream
- 1 reviews See the Breakdown
- Product Info | Add Your Review
Curls Curlicious Curls Cleansing Cream Reviews
Good but needs work...
This product is a solid cleanser for curlies. It doesn't leave my hair dry or stripped of natural oils. Plus, it defines my curls pretty well. However, it doesn't really clean the scalp very well. For weeks I walked around with an itchy scalp until I used a different sulfate free cleanser. — 4 years, 3 months agoThis review is: Helpful | Not helpful | Inappropriate | 1 of 1 people said helpful


text: Home / Products / Flare 2700K LED Path Light CO Larger View Choose background color Rollover to Zoom Close Visualize Kichler lighting and Sherwin-Williams paint all in one tool. Today's homeowners have seemingly unlimited options when it comes to colors and finishes. With so many possibilities it can be daunting to find just the right match. Not any more. Match your lighting and your wall color with our intuitive visualization tools. Choose your Sherwin-Williams Color Family Color palette belongs to Sherwin-Williams, a registered trademark of the Sherwin-Williams Company. © 2013 Sherwin-Williams Company. All rights reserved. Flare 2700K LED Path Light CO 16125CO27 (Copper) bvseo_sdk, net_sdk, 18.104.22.168 CLOUD, getAggregateRating, 93ms REVIEWS, PRODUCT bvseo-msg: The resource to the URL or file is currently unavailable.; Product Description: 2700K LED Flare Path Light - The gentle sloping texture of the Flare LED path light in Copper stands proud day or night and casts a warm white 2700K illumination. Perfect for walkways and gardens. Available Finishes Shop, Buy or Order Locally Submit Lighting Showroom Landscape Lighting Distributor Electrical Distributor Technical Information Weight:5.00 LBSSafety Rated:WetOperating Voltage Range:9-15VWidth:8.00"Height:21.00"Lamp Included:IntegratedVoltage:12VLight Source:LEDNumber of Bulbs:1Lamp Type:LEDMax Watt:4.3WColor Rendering Index:80Finish:Copper Related Documents:High Res Images 16125Download bvseo_sdk, net_sdk, 184.108.40.206 CLOUD, getReviews, 0ms REVIEWS, PRODUCT bvseo-msg: The resource to the URL or file is currently unavailable.;


text:
KrissX is a captivating and relaxing mix of puzzle game and word game. Swap letters and decipher clues to solve a series of clever puzzles. Conquer a huge variety of puzzles in Quest Mode, relax with Timeless Mode, and exercise your brain with engaging Special Puzzles. KrissX creates new puzzles each time you play, so you can keep on coming back for more - you'll never see the same puzzle twice. You can even create your own word puzzles to share with friends. KrissX is easy to play but difficult to put down!
Buy | $9.99 or 20 |
Session Price | 4 |
Developer | Blitz 1UP |
Size | 80MB, about 1 minute on broadband |
Available Languages | English |
Release Date | September 22, 2010 |
Operating System | Windows 8, Windows 7, Vista and XP |
Processor | Pentium - 600MHz or better |
DirectX Version | 7.0 or above |


text:
Doing Memphis right
Trip Start
Sep 15, 2012
Trip End
Oct 07, 2012
Our first glimpse of Memphis was a little sad because it seemed pretty deserted around this eatery. We proceeded towards Beale Street thinking we would just preview it. It turned out that Wednesday night is "Bike Night" on Beale Street which means it is shut to traffic (except bikes of course) and all the stores and restaurants stay open. It was hopping when we arrived. We love it when places stay open. It's always baffling when, and this is common in major cities from LA to San Francisco to Boston, things shut at about 7 PM. So, we attempted, unsuccessfully, to blend in with the crowd. It was very mellow with people just strolling about, checking out the bikers, and eating, drinking (open containers no problem). We decided to find some music and we ended up at Mr. Handy's Blues Hall which was featuring Brandon Santini & His Band. As the video(s) will show, it was pretty cool.
Today was a big day with Graceland as the focus of the schedule. Our crappy hotel, the Days Inn Graceland, is located one door down from the ticket stand for Graceland (across the street). So, we at least avoided paying parking. Admission is not cheap. We had a coupon which turned the 'platinum' tour into $32 instead of $36 and we were happy because this meant that for the price of the regular tour, we also got to see Elvis' private jets and his auto collection. The tour was decent. You take a little shuttle from the ticket pavilion up to Graceland and you have an audio tour through the first floor, basement, and outbuildings at Graceland. Due to Elvis' tradition of not letting anyone upstairs except family, the upstairs continues to be closed to the public. I suppose we would have seen where he died then, right? I believe he died in his bathrooom.
Some of you may know that I have a thing for Elvis. I'm not too fanatical, but I have read Priscilla Presley's autobiography, Elvis and Me (actually a page-turner!). I really like rockabilly music and anything catchy so Elvis is one of my favorites. I found the tour to be fairly surreal. I don't think I really get the whole celebrity thing though. I realize that I create celebrities in my mind to be how I want them to be and that 'reality' doesn't correspond to that image. When you see where someone ate their peanut butter and banana, it confronts the element of fantasy that seems inseparable from the creation of that image. I think I'd rather not know. Still, there was a good deal of kitschy fun involved. The house is decorated in high late 1960s/early 1970s style.
After we finished up at Graceland, we went to Stax Studios which was where Otis Redding and Booker T and the MGs recorded along with many other soul artists. We debated this or Sun Studios, but decided that we covered the country/rock side of things with Elvis. I'm glad we went to Stax. We heard a bunch of old good music and learned about the rise of the studio and its intersection with the complicated story of race relations in the 50s, 60s and 70s. It really emphasizes how important Memphis is to music. Between Stax and Sun, it's amazing how many artists recorded in Memphis from those I mentioned to Johnny Cash, Roy Orbison, Jerry Lee Lewis and the like.
We stopped by, somewhat briefly, the Peabody Hotel which is downtown Memphis for the daily marching of the ducks. For those of you who haven't heard of this tradition, it's worth looking at it (
http://en.wikipedia.org/wiki/Peabody_Hotel ). If we hadn't been so busy, we would have gone to the roof to see the new duck habitat the hotel has built. In short, there are ducks that live in the fountain in the hotel and have since the 1930s. They were trained later to march from their home on the roof down to the fountain in the morning and back at 5 PM. The ducks have been on the Tonight Show and on Sesame Street. It was extremely crowded when we showed up at 4:30. The city really seems a little deserted at times so it was surprising to see all these people in the lobby awaiting the ducks. It was cute, but a lot of fanfare for some ducks. I guess the hotel must make money off of it through concessions which is good because Memphis could use an infusion of cash and enthusiasm to keep it alive. I think I would look into staying at the Peabody if we returned. It was (re)built in the 20s and, in the style of LA's Biltmore, is very beautiful.
Following the ducks, we headed to dinner at this place, Cozy Corner which is a barbecue joint. It was as good as yesterday's chicken. I don't love messy barbecue sauce, so I had the cornish game hen "dry" or with a dry rub. It was very well-cooked and the skin was tasty without being overwhelming. Mike had the ribs which he enjoyed quite a bit (in fact, I kinda wished I had some too). Dessert, which we don't always get, was sweet potato pie and banana pudding. I *loved* the banana pudding. It was sort like a bread pudding in that it's not all pudding. There seemed to be sliced bananas, nilla wafers and whipped cream involved. The consistency was very nice. It was so good. I want more right now!
We are getting ready to pack up and head farther down South now. It's on to New Orleans tomorrow. On the way, we're traveling through Mississippi and stopping at William Faulkner's house and Eudora Welty's too. This should make our day very looooong tomorrow. It's a seven-hour drive without stoppping. Oh yes, and we are making it to Jackson, MS which is the subject of our favorite Johnny Cash tune-- "Jackson" : (Lyrics) "We got married in a fever, hotter than a pepper sprout; we've been talkin' 'bout Jackson ever since the fire went out!" I hope that we can stop for lunch there to get a feel for this town, but I don't think there's much to see there.


text:
The Nederlands and South-Africa join forces for Big Data
13 Apr 2012, 08:35 UTCPublic private partnerships get an international dimension. On April 5th, the Netherlands Organisation for Scientific Research, the South-African National Research Foundation, IBM Netherlands and IBM South-Africa signed a memorandum of understanding on joint research into advanced ICT targeted at radio astronomy.
Read more
Note: All formatting and links have been removed - click title or image to see full article.


text:
mizuka217 (Fan Art Portfolio) bunny
We now require registration to download high resolution fan art. Please take a few seconds to register absolutely free! Click here now. (Registering will also let you tell this artist how much you enjoy their work in the comments below.)
- Created
- 12/06/12
- Category
- Personal Fan Art
- Tags
- bunny, cute
- Views
- 251 views
- Votes
- 3 votes
- Favorited
- 2 members
- Member Dedication
- teapartyprincess
- Feedback
- 3 comments
- Sourced By
- Available to use (but no submissions yet)!
You're allowed to use this piece for your wallpapers and ecards if you: (1) paste this page's URL when you submit them and (2) agree not to upload your new work outside theOtaku.com - Options


text:
According to data released by Google this Monday, Jelly Bean has now surpassed Gingerbread for the first time, being the most used version of Android OS. The distribution of Jelly Bean is now 37.9%. Below is a chart showing you the distribution of each version of Android.
Version | Codename | API | Distribution |
1.6 | Dount | 4 | 0.1% |
2.1 | Éclair | 7 | 1.4% |
2.2 | Froyo | 8 | 3.1% |
2.3.3-2.3.7 | Gingerbread | 10 | 34.1% |
3.2 | Honeycomb | 13 | 0.1% |
4.0.3-4.0.4 | Ice Cream Sandwich | 15 | 23.3% |
4.1.x | Jelly Bean | 16 | 32.3% |
4.2.x | 17 | 5.6% |
Jelly Bean (Android 4.1 and Android 4.2), improves on the speed and simplicity of Android 4.1 and includes all new features – Photo Sphere and a completely redesigned camera app, new Gesture Typing keyboard, Google Now with all new cards, and much more.
Why is Jelly Bean So Popular?
The popularity of Jelly Bean seems to be caused by Samsung's Galaxy S3 and S4, which have sold millions of units in the past year. Gingerbread, released in 2010, is the operating system that's normally used on less-expensive phones. It seems that Jelly Bean will continue to grow with the integration of newer high-powered devices.
Another reason may be taht a number of OEMs pushing out Jelly Bean updates to their handsets recently, including the AT&T Galaxy S2. Gingerbread is still a close second, however, indicating a lot of older devices are still in use.
Google provides monthly updates on OS version share and this one takes into account data collected (based on Google Play store usage) over a two-week period ending today, July 8. Jelly Bean has been approaching a majority share, but this is the first time it has passed it, thanks to a 32.3 percent share for versions 4.1.X, and a 5.4 percent share for versions 4.2.X. Ice Cream Sandwich, which was sandwiched between Gingerbread and Jelly Bean, is less popular than both with the next biggest chunk of 23.3 percent.
For the moment, fragmentation is often cited as a big problem for Android, and Google has taken steps to address that, including expanding its Nexus program to include more devices. News came today that recently Google has updated Android Google map, improving search navigation and design features.
How to Recover Data on Android Phone?
Data loss has long been a problem for many people. The good news is that with some wonderful software tools we can now easily recover data on our Android devices. Tenorshare Android Data Recovery is such a tool that you can put your trust on. Just download it see how it helps you recover a bunch of photos, contacts, text messages and more from your Android phone.