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WASHINGTON (AP) — Severely wounded and still recovering, former Rep. Gabrielle Giffords begged lawmakers at an emotional hearing Wednesday to act quickly to curb firearms because "Americans are counting on you." Not everyone agreed, underscoring the national political divide over gun control.
Giffords' 80-word plea was the day's most riveting moment, delivered in a hushed, halting voice two years after the Arizona Democrat suffered head wounds in a Tucson shooting spree that killed six people — and two months after 20 first-graders and six women were slain by a gunman who invaded Sandy Hook Elementary School in Newtown, Conn. She retired from Congress last year.
But at the same session, a top official of the National Rifle Association rejected Democratic proposals to ban assault weapons and high-capacity ammunition magazines and said requiring background checks for all gun purchases would be ineffective because the Obama administration isn't doing enough to enforce the law as it is.
Even if stronger background checks did identify a criminal, "as long as you let him go, you're not keeping him from getting a gun and you're not preventing him from getting to the next crime scene," said Wayne LaPierre, the NRA's executive vice president. He said poor enforcement is "a national disgrace."
Giffords, in her brief appearance, focused on the carnage from armed assailants.
"Too many children are dying," she said at a Senate Judiciary Committee hearing. "Too many children. We must do something. It will be hard, but the time is now."
Guiding her in and remaining to testify was Mark Kelly, the retired astronaut who is Giffords' husband. The couple, who both owns guns, have formed a political action committee called Americans for Responsible Solutions that backs lawmakers who support gun restrictions.
"We're simply two reasonable Americans who realize we have a problem with gun violence and we need Congress to act," Kelly said.
Wednesday's session played out in a hearing room packed to capacity. While both sides appealed to their followers beforehand to arrive early and fill the room, most in the public audience of around 150 appeared to be gun-control sympathizers, including relatives of the shootings at Virginia Tech.
"There should be gun control," said Neeta Datt of Burtonsville, Md., who with Christa Burton of Silver Spring, Md., was first on line for public seats. Both are members of Organizing for Action, the Obama political organization that is now pushing his legislative agenda.
The hearing kicked off a year in which President Barack Obama and members of Congress are promising to make gun restrictions a top priority. Obama has already proposed requiring background checks for all gun sales and reviving both an assault weapons ban and a 10-round limit on the size of ammunition magazines, and several Democrats have introduced bills addressing those and other limitations.
After the hearing, Giffords met privately with Obama at the White House.
At the Capitol, senators' remarks during the hearing illustrated the gulf between the two parties on this issue.
Sen. Ted Cruz, R-Texas, joined others in lauding Giffords but expressed little interest in curbing firearms.
"Unfortunately in Washington, emotion I think often leads to bad policies," said Cruz, a freshman elected with strong tea party backing.
Republicans blamed the nation's gun troubles on a list of maladies including a lack of civility, violent video games and insufficient attention to people with mental problems. Sen. Charles Grassley of Iowa, the top Republican on the panel, said that while he welcomed the renewed focus on guns, "The deaths in Newtown should not be used to put forward any gun control proposal that's been floating around for years."
Democrats countered that a need to improve gun restrictions was obvious. Sen. Charles Schumer of New York said omitting gun limits from the debate "is like not including cigarettes when discussing lung cancer."
Republicans and the NRA are not the only hurdles that Democrats face in trying to push gun legislation through Congress this year. It is also unclear what several Democratic senators facing re-election in GOP-leaning states in 2014 will do, including Sens. Max Baucus of Montana, Mark Begich of Alaska and Mark Pryor of Arkansas.
Sen. Patrick Leahy, D-Vt., the committee's chairman, said he hoped his panel would write gun control legislation next month, though he did not specify what it might contain. In his opening remarks, he voiced support for requiring broader background checks that would help keep criminals and the mentally ill from obtaining firearms, and he has also introduced legislation that would make it a federal crime for someone to purchase a gun for a person who would not be legally allowed to have one.
Reflecting the emotion that the gun issue taps into nationwide, Wednesday's three-and-a-half-hour hearing featured numerous clashes between senators and some of the witnesses who testified.
"You are a large man," Gayle Trotter, a senior fellow with the conservative Independent Women's Forum, told Sen. Sheldon Whitehouse, D-R.I., as he questioned her about gun curbs he favors. "You are not a young mother who has a young child" she might have to defend, she said.
At other points, Democrats on the panel contested LaPierre's argument that criminals would simply ignore expanded requirements for background checks. Such checks are currently required for gun purchases from licensed dealers, but not some firearms bought in conjunction with gun shows or online.
"That's the point. The criminals will not go to purchase the guns because there'll be a background check. It will stop them from original purchase. You missed that point completely. It is basic," said Sen. Dick Durbin of Illinois.
Under questioning from Leahy, LaPierre said that in a reversal his organization no longer supports universal background checks for gun purchasers as it did years ago.
"Back in '99 you said, 'No loopholes, nowhere,' " said Leahy, referring to testimony delivered more than a decade ago. "Now you do not support background checks for all."
Giffords, a surprise witness, was helped to her chair as the hearing began by committee Leahy and others. She'd been working on her remarks for a week, but decided to deliver them Tuesday evening, said Pia Carusone, her former chief of staff who is now executive director for Americans for Responsible Solutions.
Kelly described the January 2011 attack on Giffords and others and described her battle to regain basic skills.
"Gabby's gift for speech is a distant memory," he told the senators. "She struggles to walk, and she is partially blind. Her right arm is completely paralyzed. And a year ago she left a job she loved serving the people of Arizona."
Toward the end of the hearing, Kelly said he had just gotten word of another Arizona shooting that occurred during Wednesday's session. That shooting in a Phoenix office building left three people wounded, and the shooter was being hunted.

subset: commoncrawl


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Our Abpromise guarantee covers the use of ab74004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
ab74004 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"


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Charles Krauthammer: On the fiscal cliff, Obama plays politics
This is why he sent Treasury Secretary Tim Geithner to the Republicans to convey not a negotiating offer but a demand for unconditional surrender. House Speaker John Boehner had made a peace offering of $800 billion in new revenues. Geithner pocketed Boehner's $800 billion, doubled it to $1.6 trillion, offered risible cuts that in 2013 would actually be exceeded by new stimulus spending, and then demanded that Congress turn over to the President all power over the debt ceiling.
Boehner was stunned. Mitch McConnell laughed out loud. In nobler days, they'd have offered Geithner a pistol and an early-morning appointment at Weehawken. Alas, Boehner gave again, coming back a week later with spending-cut suggestions - as demanded by Geithner - only to have them dismissed with a wave of the hand.
What's going on here? Having taken Boehner's sword, and then his shirt, Obama sent Geithner to demand Boehner's trousers. Perhaps this is what Obama means by a balanced approach.
He pretends that Boehner's offer to raise revenues by eliminating deductions rather than by raising rates is fiscally impossible. But on July 22, 2011, Obama had said that "$1.2 trillion in additional revenues ... could be accomplished without hiking tax rates, but could simply be accomplished by eliminating loopholes, eliminating some deductions and engaging in a tax reform process." Which is exactly what the Republicans are offering today.
As for the alleged curative effect on debt of Obama's tax-rate demand - the full rate hike on the "rich" would have reduced the 2012 deficit from $1.10 trillion to $1.02 trillion.
That's a joke, a rounding error.
Such nonsense abounds because Obama's objective in these negotiations is not economic but political: not to solve the debt crisis but to fracture the Republican majority in the House. Get Boehner to cave, pass the tax hike with Democratic votes provided by Minority Leader Nancy Pelosi, and let the Republican civil war begin.
It doesn't even matter whether Boehner gets deposed as speaker. Either way, the Republican House would be neutered, giving Obama a free hand to dominate Washington and fashion the entitlement state of his liking.
This is partisan zero-sum politics. Nothing more. Obama has never shown interest in genuine debt reduction. He does nothing for two years, then spends the next two ignoring his own debt-reduction commission. In less than four years, he has increased U.S. public debt by a staggering 83 percent. As a percentage of GDP, the real marker of national solvency, it has spiked from 45 percent to 70 percent.
Obama has never once publicly suggested a structural cut in entitlements. On the contrary, he created an entirely new entitlement - Obamacare - that, according to the CBO, will increase spending by $1.7 trillion.
What's he thinking? Doesn't Obama see looming ahead the real economic cliff - a European-like collapse under the burden of unsustainable debt? Perhaps, but he wants to complete his avowedly transformational social-democratic agenda first, and let his successors - likely Republican - act as tax collectors on the middle class (where the real money is) and takers of subsidies from the mouths of babes.
Or possibly Obama will get fiscal religion and undertake tax and entitlement reform in his second term - but only after having destroyed the Republican opposition so that he can carry out the reformation on his own ideological terms.
What should Republicans do? Stop giving stuff away. If Obama remains intransigent, let him be the one to take us over the cliff. And then let the new House, which is sworn in weeks before the President, immediately introduce and pass a full across-the-board restoration of the Bush tax cuts.
Obama will counter with the usual all-but-the-rich tax cut - as the markets gyrate and the economy begins to wobble under his feet.
Result? We're back to square one, but with a more level playing field. The risk to Obama will be rising and the debt ceiling will be looming. Most important of all, however, Republicans will still be in possession of their unity, their self-respect - and their trousers.
Charles Krauthammer is a columnist for The Washington Post.


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