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Overview: A Body-Building Plan A human embryo at about 7 weeks after conception shows development of distinctive features Development occurs at many points in the life cycle of an animal
EMBRYONIC DEVELOPMENT Sperm Adult frog Egg Metamorphosis Larval stages Zygote Blastula Gastrula Tail-bud embryo FERTILIZATION CLEAVAGE GASTRULATION ORGANO- GENESIS
Model Organisms Although animals display different body plans, they share many basic mechanisms of development and use a common set of regulatory genes Biologists use model organisms to study development, chosen for the ease with which they can be studied in the laboratory
Concept 47.1 Fertilization and cleavage initiate embryonic development Fertilization is the formation of a diploid zygote from a haploid egg and sperm
Fertilization Molecules and events at the egg surface play a crucial role in each step of fertilization Sperm penetrate the protective layer around the egg Receptors on the egg surface bind to molecules on the sperm surface Changes at the egg surface prevent polyspermy, the entry of multiple sperm nuclei into the egg
The Acrosomal Reaction The acrosomal reaction is triggered when the sperm meets the egg The acrosome at the tip of the sperm releases hydrolytic enzymes that digest material surrounding the egg
Gamete contact and/or fusion depolarizes the egg cell membrane and sets up a fast block to polyspermy
The Cortical Reaction Fusion of egg and sperm also initiates the cortical reaction Seconds after the sperm binds to the egg, vesicles just beneath the egg plasma membrane release their contents and form a fertilization envelope The fertilization envelope acts as the slow block to polyspermy
The Cortical Reaction The cortical reaction requires a high concentration of Ca 2 ions in the egg The reaction is triggered by a change in Ca 2 concentration Ca 2 spread across the egg correlates with the appearance of the fertilization envelope
10 sec after fertilization 25 sec 35 sec 1 min 500 m 30 sec 20 sec 10 sec after fertilization 1 sec before fertilization Point of sperm nucleus entry Spreading wave of Ca 2 Fertilization envelope EXPERIMENT RESULTS CONCLUSION
Egg Activation The rise in Ca 2+ in the cytosol increases the rates of cellular respiration and protein synthesis by the egg cell With these rapid changes in metabolism, the egg is said to be activated The proteins and mRNAs needed for activation are already present in the egg The sperm nucleus merges with the egg nucleus and cell division begins
Fertilization in Mammals Fertilization in mammals and other terrestrial animals is internal Secretions in the mammalian female reproductive tract alter sperm motility and structure This is called capacitation, and must occur before sperm are able to fertilize an egg
Sperm travel through an outer layer of cells to reach the zona pellucida, the extracellular matrix of the egg When the sperm binds a receptor in the zona pellucida, it triggers a slow block to polyspermy No fast block to polyspermy has been identified in mammals In mammals the first cell division occurs 12 36 hours after sperm binding The diploid nucleus forms after this first division of the zygote
Zona pellucida Follicle cell Sperm basal body Sperm nucleus Cortical granules
Cleavage Fertilization is followed by cleavage, a period of rapid cell division without growth Cleavage partitions the cytoplasm of one large cell into many smaller cells called blastomeres The blastula is a ball of cells with a fluid-filled cavity called a blastocoel
Figure 47.6a (a) Fertilized egg
Figure 47.6b (b) Four-cell stage
Figure 47.6c (c) Early blastula
Figure 47.6d (d) Later blastula
Cleavage Patterns In frogs and many other animals, the distribution of yolk (stored nutrients) is a key factor influencing the pattern of cleavage The vegetal pole has more yolk; the animal pole has less yolk The difference in yolk distribution results in animal and vegetal hemispheres that differ in appearance
The first two cleavage furrows in the frog form four equally sized blastomeres The third cleavage is asymmetric, forming unequally sized blastomeres Holoblastic cleavage, complete division of the egg, occurs in species whose eggs have little or moderate amounts of yolk, such as sea urchins and frogs Meroblastic cleavage, incomplete division of the egg, occurs in species with yolk-rich eggs, such as reptiles and birds
Figure 47.7a-5 Zygote 2-cell stage forming 4-cell stage forming 8-cell stage Vegetal pole Blastula (cross section) Gray crescent Animal poleBlastocoel
Animal pole 0.25 mm 8-cell stage (viewed from the animal pole) 0.25 mm Blastocoel Blastula (at least 128 cells)
Regulation of Cleavage Animal embryos complete cleavage when the ratio of material in the nucleus relative to the cytoplasm is sufficiently large
Concept 47.2 Morphogenesis in animals involves specific changes in cell shape, position, and survival After cleavage, the rate of cell division slows and the normal cell cycle is restored Morphogenesis, the process by which cells occupy their appropriate locations, involves Gastrulation, the movement of cells from the blastula surface to the interior of the embryo Organogenesis, the formation of organs
Gastrulation Gastrulation rearranges the cells of a blastula into a three-layered embryo, called a gastrula The three layers produced by gastrulation are called embryonic germ layers The ectoderm forms the outer layer The endoderm lines the digestive tract The mesoderm partly fills the space between the endoderm and ectoderm Each germ layer contributes to specific structures in the adult animal
ECTODERM (outer layer of embryo) MESODERM (middle layer of embryo) ENDODERM (inner layer of embryo) Epidermis of skin and its derivatives (including sweat glands, hair follicles) Epithelial lining of digestive tract and associated organs (liver, pancreas) Epithelial lining of respiratory, excretory, and reproductive tracts and ducts Germ cells Jaws and teeth Pituitary gland, adrenal medulla Nervous and sensory systems Skeletal and muscular systems Circulatory and lymphatic systems Excretory and reproductive systems (except germ cells) Dermis of skin Adrenal cortex Thymus, thyroid, and parathyroid glands
Gastrulation in Sea Urchins Gastrulation begins at the vegetal pole of the blastula Mesenchyme cells migrate into the blastocoel The vegetal plate forms from the remaining cells of the vegetal pole and buckles inward through invagination The newly formed cavity is called the archenteron This opens through the blastopore, which will become the anus
Animal pole Blastocoel Mesenchyme cells Vegetal plate Vegetal pole Blastocoel Filopodia Mesenchyme cells Blastopore Archenteron 50 m Ectoderm Mouth Mesenchyme (mesoderm forms future skeleton) Blastopore Blastocoel Archenteron Digestive tube (endoderm) Anus (from blastopore) Key Future ectoderm Future mesoderm Future endoderm Figure 47.9
Gastrulation in Frogs Frog gastrulation begins when a group of cells on the dorsal side of the blastula begins to invaginate This forms a crease along the region where the gray crescent formed The part above the crease is called the dorsal lip of the blastopore
Cells on the Embryo Cells continue to move from the embryo surface into the embryo by involution These cells become the endoderm and mesoderm Cells on the embryo surface will form the ectoderm
Key Future ectoderm Future mesoderm Future endoderm SURFACE VIEW CROSS SECTION Animal pole Vegetal pole Early gastrula Blastocoel Dorsal lip of blasto- pore Blastopore Dorsal lip of blastopore Blastocoel shrinking Archenteron Blastocoel remnant Ectoderm Mesoderm Endoderm Blastopore Yolk plug Blastopore Late gastrula 321 Figure 47.10
Gastrulation in Chicks Prior to gastrulation, the embryo is composed of an upper and lower layer, the epiblast and hypoblast, respectively During gastrulation, epiblast cells move toward the midline of the blastoderm and then into the embryo toward the yolk
Gastrulation in Chicks The midline thickens and is called the primitive streak The hypoblast cells contribute to the sac that surrounds the yolk and a connection between the yolk and the embryo, but do not contribute to the embryo itself
Future ectoderm Migrating cells (mesoderm) Blastocoel Epiblast YOLK Endoderm Hypoblast Primitive streak Fertilized egg Primitive streak Embryo Yolk Figure 47.11
Gastrulation in Humans Human eggs have very little yolk A blastocyst is the human equivalent of the blastula The inner cell mass is a cluster of cells at one end of the blastocyst The trophoblast is the outer epithelial layer of the blastocyst and does not contribute to the embryo, but instead initiates implantation
After Implantation Following implantation, the trophoblast continues to expand and a set of extraembryonic membranes is formed These enclose specialized structures outside of the embryo Gastrulation involves the inward movement from the epiblast, through a primitive streak, similar to the chick embryo
Blastocyst reaches uterus. 1 Uterus Endometrial epithelium (uterine lining) Inner cell mass Trophoblast Blastocoel
Blastocyst implants (7 days after fertilization). Maternal blood vessel Expanding region of trophoblast Epiblast Hypoblast Trophoblast 2
Extraembryonic membranes start to form (10–11 days), and gastrulation begins (13 days). Expanding region of trophoblast Amniotic cavity Epiblast Hypoblast Yolk sac (from hypoblast) Extraembryonic mesoderm cells (from epiblast) Chorion (from trophoblast) 3
Gastrulation has produced a three-layered embryo with four extraembryonic membranes. Amnion Chorion Ectoderm Mesoderm Endoderm Yolk sac Extraembryonic mesoderm Allantois 4
Developmental Adaptations of Amniotes The colonization of land by vertebrates was made possible only after the evolution of The shelled egg of birds and other reptiles as well as monotremes (egg-laying mammals) The uterus of marsupial and eutherian mammals
In both adaptations, embryos are surrounded by fluid in a sac called the amnion This protects the embryo from desiccation and allows reproduction on dry land Mammals and reptiles including birds are called amniotes for this reason The four extraembryonic membranes that form around the embryo The chorion functions in gas exchange The amnion encloses the amniotic fluid The yolk sac encloses the yolk The allantois disposes of waste products and contributes to gas exchange
Organogenesis During organogenesis, various regions of the germ layers develop into rudimentary organs Early in vertebrate organogenesis, the notochord forms from mesoderm, and the neural plate forms from ectoderm
Figure 47.13a Neural folds 1 mm Neural fold Neural plate Notochord Ectoderm Mesoderm Endoderm Archenteron (a) Neural plate formation
(b) Neural tube formation Neural fold Neural plate The neural plate soon curves inward, forming the neural tube The neural tube will become the central nervous system (brain and spinal cord)
(b) Neural tube formation Neural fold Neural plate Neural crest cells The neural plate soon curves inward, forming the neural tube The neural tube will become the central nervous system (brain and spinal cord)
(b) Neural tube formation Neural fold Neural plate Neural crest cells Outer layer of ectoderm Neural crest cells Neural tube The neural plate soon curves inward, forming the neural tube The neural tube will become the central nervous system (brain and spinal cord)
Neural crest cells develop along the neural tube of vertebrates and form various parts of the embryo (nerves, parts of teeth, skull bones, and so on) Mesoderm lateral to the notochord forms blocks called somites Lateral to the somites, the mesoderm splits to form the coelom (body cavity) Organogenesis in the chick is quite similar to that in the frog
Figure 47.13c (c) Somites Eye Somites Tail bud SEM Neural tube Notochord Coelom Neural crest cells Somite Archenteron (digestive cavity) 1 mm
Neural folds 1 mm Eye Somites Tail bud SEM 1 mm
Figure 47.14a Neural tube Notochord Archenteron Lateral fold These layers form extraembryonic membranes. Yolk stalk YOLK Yolk sac Somite Coelom Endoderm Mesoderm Ectoderm (a) Early organogenesis
(b) Late organogenesis Eye Forebrain Heart Blood vessels Somites Neural tube
The mechanisms of organogenesis in invertebrates are similar, but the body plan is very different For example, the neural tube develops along the ventral side of the embryo in invertebrats, rather than dorsally as occurs in vertebrates
Mechanisms of Morphogenesis Morphogenesis in animals but not plants involves movement of cells Reorganization of the cytoskeleton is a major force in changing cell shape during development For example, in neurulation, microtubules oriented from dorsal to ventral in a sheet of ectodermal cells help lengthen the cells along that axis
Ectoderm Neural plate Microtubules Actin filaments Neural tube
The cytoskeleton promotes elongation of the archenteron in the sea urchin embryo This is convergent extension, the rearrangement of cells of a tissue that cause it to become narrower (converge) and longer (extend) Convergent extension occurs in other developmental processes The cytoskeleton also directs cell migration Extension Convergence
Programmed Cell Death Programmed cell death is also called apoptosis At various times during development, individual cells, sets of cells, or whole tissues stop developing and are engulfed by neighboring cells For example, many more neurons are produced in developing embryos than will be needed Extra neurons are removed by apoptosis
Concept 47.3: Cell fate specification Determination is the term used to describe the process by which a cell or group of cells becomes committed to a particular fate Differentiation refers to the resulting specialization in structure and function Cells in a multicellular organism share the same genome Differences in cell types is the result of the expression of different sets of genes
Fate Mapping Fate maps are diagrams showing organs and other structures that arise from each region of an embryo Classic studies using frogs indicated that cell lineage in germ layers is traceable to blastula cells
Later studies of C. elegans used the ablation (destruction) of single cells to determine the structures that normally arise from each cell The researchers were able to determine the lineage of each of the 959 somatic cells in the worm
First cell division Zygote Hatching Time after fertilization (hours) Intestine Mouth EggsVulva Anus 1.2 mm ANTERIOR POSTERIOR Nervous system, outer skin, muscula- ture Muscula- ture, gonads Outer skin, nervous system Germ line (future gametes) Musculature 10 0
Germ cells are the specialized cells that give rise to sperm or eggs Complexes of RNA and protein are involved in the specification of germ cell fate In C. elegans, such complexes are called P granules, persist throughout development, and can be detected in germ cells of the adult worm
Partioning P granules are distributed throughout the newly fertilized egg and move to the posterior end before the first cleavage division With each subsequent cleavage, the P granules are partitioned into the posterior-most cells P granules act as cytoplasmic determinants, fixing germ cell fate at the earliest stage of development
Newly fertilized egg Zygote prior to first division Two-cell embryo Four-cell embryo 2134
Axis Formation A body plan with bilateral symmetry is found across a range of animals Exhibits asymmetry across the dorsal-ventral and anterior-posterior axes where the right-left axis is largely symmetrical The anterior-posterior axis of the frog embryo is determined during oogenesis The animal-vegetal asymmetry indicates where the anterior-posterior axis forms The dorsal-ventral axis is not determined until fertilization
After Sperm meets Egg Upon fusion of the egg and sperm, the egg surface rotates with respect to the inner cytoplasm This cortical rotation brings molecules from one area of the inner cytoplasm of the animal hemisphere to interact with molecules in the vegetal cortex This leads to expression of dorsal- and ventral-specific gene expression
Dorsal Right AnteriorPosterior Ventral Left (a) The three axes of the fully developed embryo (b) Establishing the axes Animal hemisphere Vegetal hemisphere Animal pole Vegetal pole Point of sperm nucleus entry Gray crescent Pigmented cortex Future dorsal side First cleavage Figure 47.21
Orientation In chicks, gravity is involved in establishing the anterior-posterior axis Later, pH differences between the two sides of the blastoderm establish the dorsal- ventral axis In mammals, experiments suggest that orientation of the egg and sperm nuclei before fusion may help establish embryonic axes
Restricting Developmental Potential Hans Spemann performed experiments to determine a cell's developmental potential (range of structures to which it can give rise) Embryonic fates are affected by distribution of determinants and the pattern of cleavage The first two blastomeres of the frog embryo are totipotent (can develop into all the possible cell types)
Control egg (dorsal view) 2 1a1b Gray crescent Control group Experimental group Experimental egg (side view) Gray crescent Thread Normal Belly piece EXPERIMENT RESULTS
In mammals, embryonic cells remain totipotent until the 8-cell stage, much longer than other organisms Progressive restriction of developmental potential is a general feature of development in all animals In general tissue-specific fates of cells are fixed by the late gastrula stage Cell Fate Determination and Pattern Formation by Inductive Signals As embryonic cells acquire distinct fates, they influence each other's fates by induction
Formation of the Vertebrate Limb Inductive signals play a major role in pattern formation, development of spatial organization The molecular cues that control pattern formation are called positional information This information tells a cell where it is with respect to the body axes It determines how the cell and its descendents respond to future molecular signals The wings and legs of chicks, like all vertebrate limbs, begin as bumps of tissue called limb buds
Limb buds 50 m Anterior Limb bud AER ZPA Posterior Apical ectodermal ridge (AER) (a) Organizer regions (b) Wing of chick embryo Digits Anterior Proximal Dorsal Posterior Ventral Distal 2 3 4
The embryonic cells in a limb bud respond to positional information indicating location along three axes Proximal-distal axis Anterior-posterior axis Dorsal-ventral axis One limb-bud regulating region is the apical ectodermal ridge (AER) The AER is thickened ectoderm at the bud's tip The second region is the zone of polarizing activity (ZPA) The ZPA is mesodermal tissue under the ectoderm where the posterior side of the bud is attached to the body
Cilia and Cell Fate Ciliary function is essential for proper specification of cell fate in the human embryo Motile cilia play roles in left-right specification Monocilia (nonmotile cilia) play roles in normal kidney development
Figure 47.UN05 Species: Stage:
Figure 47.UN06
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Professional person to produce an excellent assignment in Education
This project was awarded to rafaelnadal for $90 USD.Get free quotes for a project like this
Project Budget$30 - $250 USD
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Project Description
I need a professional person to produce an excellent assignment of 3000 words as follows
PLEASE..Do not bid if you are not specialist in the field OR you are not able to have this done perfectly.
Select and critically analyse a test to evaluate a test to evaluate its suitability for its intended purpose or other uses.
Select a test with which you are familiar. This may be form one of the large testing agencies such as Educational Testing Service (ETS, see [url removed, login to view]) or Cambridge ESOL (see [url removed, login to view]) .
Analyse the test and critically evaluate its suitability for its stated purpose or other use to which the test is put.
Will support you with the specific research question >> I need this to be done in 2-3 days.
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Westcott Elite Straight ScissorsScissors
3.50" Cutting Length - Pointed - Straight-left/right - Stainless Steel, Plastic - Orange, Satin - View Complete Details
Product Number: ACM41318
In Stock - Hurry, only 162 left.
Estimated Delivery - 1 business day(s) - Tue 2/28 2 business days - Tue 2/28
Shipping - This item is eligible for Free Shipping on orders over $45
Note: Must buy in quantities of 6 starting at 66.
Estimated Delivery 10 business day(s) - Mon 3/13 - The products will be shipped directly from the Manufacturer and will require additional time for delivery. 10 business days - Mon 3/13 - The products will be shipped directly from the Manufacturer and will require additional time for delivery.
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Product Details
Westcott Elite Straight Scissors - Elite line of shears has precision-ground, corrosion-resistant stainless steel blades. Sharp, double-honed stainless steel blades retain edge after long use. Scissors features satin finish blades with inside edge and bevel grinds. Specially designed handles work with your hands whether you're left or right-handed.
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Dream Clean Solutions
Bolton, Borough of Bolton, Greater Manchester
Dream Clean Solutions Details
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Sales Telephone:07885 913357
About Dream Clean Solutions
We offer a local domestic cleaning service for either regular cleans or one off cleans. Priced at £12 per hour - we provide all cleaning products and only use hospital graded cleaning products. All staff are insured, police checked and wear uniforms.
We also offer an ironing service at £15 per hour - includes collect & return service and free hangers.
All areas of Bolton covered.
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Making Mice
Standardizing Animals for American Biomedical Research, 1900-1955
By (author) Karen Rader
Format: Hardback
Normal Price: $146.00
Your Price: $131.40 AUD, inc. GST
Shipping: $7.95 per order
You Save: $14.60! (10% off normal price)
Plus...earn $6.57 in Boomerang Bucks
Availability: Available to Backorder, No Due Date for Supply
Making Mice by Karen Rader
Book Awards
Book DescriptionMaking Mice blends scientific biography, institutional history, and cultural history to show how genetically standardized mice came to play a central role in contemporary American biomedical research. Karen Rader introduces us to mouse "fanciers" who bred mice for different characteristics, to scientific entrepreneurs like geneticist C. C. Little, and to the emerging structures of modern biomedical research centered around the National Institutes of Health. Throughout Making Mice, Rader explains how the story of mouse research illuminates our understanding of key issues in the history of science such as the role of model organisms in furthering scientific thought. Ultimately, genetically standardized mice became icons of standardization in biomedicine by successfully negotiating the tension between the natural and the man-made in experimental practice. This book will become a landmark work for its understanding of the cultural and institutional origins of modern biomedical research. It will appeal not only to historians of science but also to biologists and medical researchers.
Buy Making Mice book by Karen Rader from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9780691016368
(229mm x 152mm x 19mm)
Pages: 312
Imprint: Princeton University Press
Publisher: Princeton University Press
Publish Date: 1-Mar-2004
Country of Publication: United States
» Have you read this book? We'd like to know what you think about it - write a review about Making Mice book by Karen Rader and you'll earn 50c in Boomerang Bucks loyalty dollars (you must be a member - it's free to sign up!)
Author Biography - Karen Rader
Karen Rader is Marilyn Simpson Chair of Science and Society at Sarah Lawrence College.
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Who's a pretty girl? JoJo's a pretty girl! Here is JoJo in her Halloween photo shoot. She is a very sweet and smart Chihuahua/Terrier Mix who is 3 years old and weighs 9 pounds. She loves to have fun and play with other dogs. One of her favorite past times is to cuddle up next to her person. If you would like to make JoJo part of your family, please fill out an application online at www.lapdogrescue.org and include her name at the top.
Our adoption fees range from $175(the majority) to $300 and include spay/neuter, shots and microchip. Puppies less than 6 months old are $225 and special or dogs requiring additional medical care are $300. A home visit is required prior to completing the adoption process to ensure the best match for the dog and the adopting family.
Thank You!
Your message has been sent to Lap Dog Rescue of New Mexico.
You'll receive a copy, too, at to help you keep track of which pets you've inquired about, and which shelters and rescues you've emailed.
NOTE: Some shelters have physical locations you can visit; some of these shelters may only have pets for a limited time, so please do not wait for a reply—just go visit the shelter! Other organizations are rescue groups run by busy volunteers who may take a while to reply. You can find information about the shelter or rescue group caring for this pet, and their adoption procedures, on the pet's details page on Adopt-a-Pet.com.


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Sydney, NSW
Australia Souvenir is a unique online souvenirs shop that features the traditional artworks of Australian Aborigines. Free Shipping Worldwide! We off…
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Creamy Seafood Pasta
|Vermouth cooking wine
|1 Cup (16 tbs)
|1 Cup (16 tbs)
|1⁄2 Pound, thawed (Use Fresh Or Frozen)
|Uncooked shrimp
|1⁄2 Pound, peeled, deveined (Use Fresh Or Frozen)
|Whipping cream
|1 Cup (16 tbs)
|1 Clove (5 gm), minced
|2 Teaspoon
|Lemon juice
|1 Tablespoon
|Freshly grated parmesan cheese
|2 Tablespoon
|2 Tablespoon
|Shredded zucchini
|2 Cup (32 tbs) (Use 2 Medium Sized)
|Spinach fettuccine
|8 Ounce, cooked, drained
1) Take a large saucepan and bring cooking wine and water to a boil in it.
2) Stir in scallops and shrimp and cook for 2 to 3 minutes until shrimp turns pink in color.
3) Save 1 cup liquid on draining and keep aside the mixture warm.
4) To the saucepan, add the saved liquid followed by whipping cream and garlic.
5) Allow the mixture to come to a boil and then reduce the cooking temperature.
6) Boil for about 15 minutes over medium heat, stirring occasionally, until sauce is thickened and reduced by half.
7) Take a small dish and mix well cornstarch and lemon juice in it.
8) Mix the cornstarch mixture with sauce and cook for about 10 minutes over medium heat until sauce boils and thickens and then add in cheese.
9) Take a small saucepan; melt margarine over high heat and sauté zucchini in it for 1 minute until softened.
10) Mix the sautéed zucchini and seafood mixture with sauce.
11) Serve hot over cooked fettuccine.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size
Calories 1436 Calories from Fat 530
% Daily Value*
Total Fat 61 g94.3%
Saturated Fat 37.3 g186.6%
Trans Fat 0 g
Cholesterol 420.9 mg140.3%
Sodium 1472.2 mg61.3%
Total Carbohydrates 116 g38.5%
Dietary Fiber 7.5 g30%
Sugars 19.6 g
Protein 64 g127.3%
Vitamin A 21.8% Vitamin C 51.6%
Calcium 39.4% Iron 20.6%
*Based on a 2000 Calorie diet


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Netherland dwarf rabbits will definitely throw you through a loop with their strange behaviors. Rabbits are unlike dogs and cats in the ways they communicate with their caregivers and playmates. Thumping, humming and licking are all par for the course with these little crazy animals. Furthermore, rabbits have a rather disgusting habit of eating their own waste products, which actually protects their health. Read on to learn more information.
Loud Thumping
When rabbits perceive danger, they will use their little legs to thump a warning to the group. After first bringing your rabbit home, every little noise may cause your rabbit to sit in a guarded position and thump away. Eventually, your rabbit will become accustomed to household sounds and only thump when startled. You can calm your rabbit by speaking in a gentle voice or softly petting your rabbit's sides. Suddenly picking up your rabbit may cause him or her to bite or urinate in fear.
Soft Humming
When rabbits want to show affection, they may softly hum or buzz while making circles around their beloved. Unaltered male and female rabbits frequently exhibit this behavior when ready to mate. After having your rabbit neutered or spayed, he or she may still buzz or hum after receiving a treat or attention from you. Humming from an altered rabbit just signals excitement and adoration for their caregivers. Some rabbits even make a sudden honking sound in response to petting, holding or feeding.
Determined Licking
Rabbits will frequently groom each other when living in bonded pairs. If your rabbit considers you one of the pack, or warren, you will receive your fair share of grooming as well. Rabbits often start the grooming process by licking and gently nibbling, which is similar to the way they remove debris and knotted hairs from their coats. Some rabbits will even rub their chin on you to claim you with their scent. You can return the favor by gently combing your bunny's fur when he or she comes over for a grooming session.
Waste Eating
Rabbits eat their own waste products on purpose. In fact, they produce a special kind of waste, called cecotropes, which are filled with nutrients left undigested by the intestines. Although cecotropes are not actually feces, they look awful close to the real thing. Eating these waste particles gives the bunny's body a second chance to absorb the nutrients. The waste products can even protect the rabbit from harmful conditions, like viral infections and vitamin deficiencies. Since most bunnies consume their cecotropes at night, you may not notice this behavior right away.
Checking Out Strange Behaviors
Whenever you feel unsure about your rabbit's behaviors, or overall health condition, it doesn't hurt to go into the vet for a checkup. Vets not only diagnose and treat health problems; they are also available to explain behaviors in an effort to help people understand their pets better. Vets can even help you figure out ways to eliminate behaviors that are disturbing, distressing or dangerous.


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Property Queensland
Property, Property Investment, Investment Property
I have now made four property investments through Graeme Shiels since 2007.He has always had the widest range of properties to choose from and is always careful to make the best possible recommendations for me.When he first made the NRAS investment strategy available to me, I instantly understood the value of superior cashflow to me and have now bought two NRAS properties.My first NRAS property, on completion, was tenanted in less than a week. And the building process is underway for my second NRAS property. I come up to Brisbane once a year to inspect my properties and look at the best properties that Graeme has available for me. I would certainly recommend Graeme Shiels to anyone looking for the best investment strategy available at any time. Jason, Builder - SydneyGraeme, a big thank you to you. I could not believe that everything went so well and so smoothly with the purchase of my NRAS investment property in Ipswich. No worries at all during the whole process. My NRAS property was tenanted within 5 days and I had to slow down the tenanting process, to ensure that the tenant did not move in before I could come up from Sydney to inspect my completed property.The services I received from Graeme Shiels have been tremendous. I am very fortunate to have Graeme to look after me and I enthusiastically recommend Graeme Shiels to anyone considering making an investment in an NRAS property.Jian - IT Officer, SydneyWell we are nothing short of amazed at the service we have received from Graeme Shiels, since we ordered our first NRAS property investment. He has done more than we ever expected he would. His support and knowledge has just been great. From purchase of land to completion of the new home, Graeme has been there encouraging us and guiding us where necessary. We now have a fully tenanted NRAS investment property. Within four days of completing and returning the Head Lease to NRAS, we had a tenant move in. Just incredible! As I said a number of times on the phone to him, Graeme certainly went the extra mile for us. Now we are looking forward to reaping the rewards. We highly recommend Graeme Shiels and the knowledge that he has of NRAS.Thanks again.Bev and Hugh, School teacher, Country QLD
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Davidson Bikes
Davidson Bikes is located at 110 S. Federal Hwy in Pompano Beach, FL.
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(855) 741-9027
110 S. Federal Hwy
Pompano Beach, FL 33062


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if you dont stop i will pull the trigger
The if you dont stop i will pull the trigger meme was made with the le gun guy meme meme generator.
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Audio-Assault GrindMachine v1.5.5 VST VST3 AU RTAS AAX WiNOSX-SYNTHiC4TE
Audio-Assault GrindMachine v1.5.5 VST VST3 AU RTAS AAX WiN/OSX-SYNTHiC4TE | 29 MB
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It is known news that Allari Naresh has teamed up with Indraganti Mohana Krishna for a comedy entertainer titled Bandipotu. The trailers have created a decent hype for the film and Allari Naresh is pretty confident about the film's success.
He has been promoting the film quite aggressively and has pinned a lot of hopes on this project. Produced by Naresh's home banner, Bandipotu is set for a grand release tomorrow. Esha plays the female lead and Kalyan Koduri scores music.
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Remember hitchBOT? The adorable robot was left for scraps by the side of a road while traveling through the U.S.
Once again hitchBOT is venturing out in the world to test the kindness of strangers and they are trying to figure out what Canadian identity means.
The staff and students at Erin Woods Elementary School in Calgary built 22 hitchBOTS as part of their class assignment and one is arriving in Toronto today!
Laura Elphinstone is one of the teachers from Erin Woods Elementary School to discuss the social experiment.
Follow hitchBOT on twitter @HitchbotWoods


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The Colbert Report 2014 02 19 Alexander Payne 720p HDTV x264-BATV
The Colbert Report 2014 02 19 Alexander Payne 720p HDTV x264-BATV
Language: English
Genres: Comedy / News
00:21:28 | 4734 kb/s | 1280?720 | 29.97 fps(r) (eng) | ac3, 48000 Hz, stereo | 726.92 MiB
An, offshoot of The Daily Show, The Colbert Report (the Ts in Colbert and in Report are silent) is a parody of shows like 揟he O扲eilly Factor.?Colbert begins the show with teasers about the show抯 content and then the credits go by, with a giant eagle and the stars and stripes featured predominantly. Colbert will pepper commentaries with phrases like, 揕ook, I don抰 have time to read books,?or, 揚resident Bush isn抰 a head thinker, he抯 a gut thinker.?Aspects of talking head programs are exaggerated and Colbert presents a host overwhelmed by his own importance. Guests do not walk on-stage but are seated at a small table to the side. Colbert runs over to them, encouraging the audience to applaud and high five him as he passes. He is then likely to begin an interview by asking a guest something like, �xplain evolution in 20 seconds,?or 揑sn抰 everyone who disagrees with the president guilty of treason??Colbert is also very clear about our number one threat, 揃ears.?His distrust ?br />
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Is Culet Jewellery incorrect or missing key business information? Update your Hotfrog listing today.
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Hotfrog NewZealand provides information regarding Culet Jewellery in Auckland. Culet Jewellery is located at 166 Hurstmere Road, Auckland Auckland 6020 and provides Retail services. Contact them on 09-489 3673 or by visiting them on their website http://www.culet.co.nz/.
Is Culet Jewellery in 166 Hurstmere Road, Auckland Auckland 6020 your business? Claim your listing and attract more leads by adding more content, photos and other business details.
We have more Retail services in Auckland available on Hotfrog NewZealand. You can update your search for Auckland Retail by location, keyword or service options.
30 Huron St Takapuna Auckland 0622
8 harbour lights close Auckland 1008
Ice Machine Rentals Ltd, P O Box 201 Auckland 1140
2/2 Lansford Cresent, Avondale Auckland 1007
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Wiggle Lyrics – Jason Derulo – Platinum Hits
Wiggle (Ft. Snoop Dogg) Song Is From A Sound Track Album Platinum Hits (2016) Sung By An American Singer Jason Derulo. This Song Is Written By Desrouleaux , Frederic. Produced By Ricky Reed. This Album Is Recorded On Beluga Heights And Warner Bros. There Play Duration Of This Track Is 3:13. Wiggle (Ft. Snoop Dogg) Lyrics Are Available Here.
Wiggle Lyrics
(Ft. Snoop Dogg)
(Intro – Jason Derülo & Snoop Dogg)
Hey yo Jason
Say somethin' to her
Holla at her
I got one question
How do you fit all that in them jeans?
You know what to do with that big fat butt
Wiggle wiggle wiggle
Wiggle wiggle wiggle
Wiggle wiggle wiggle
Just a little bit
(Verse 1: Jason Derülo)
Patty cake patty cake with no hands
Got me in this club, making wedding plans
If I take pictures while you do your dance
I could make you famous on Instagram
Hot damn it, your booty like two planets
Go ahead, and go ham sandwich
Woah I can't stand it
Cause you know what to do with that big fat butt
(Verse 2: Jason Derülo)
Cadillac Cadillac, pop that trunk
Let's take a shot, Alley oop that donk
Tired of working that nine to five
Oh, baby let me come and change your life
(Pre-Hook & Hook)
(Wiggle wiggle wiggle)
(Shake it shake it girl)
(Little bit, little bit, little bit)
(Verse 3: Snoop Dogg)
Shake what your mamma gave you, misbehave you
I just wanna strip you, dip you, flip you, bubble bathe you
What they do
Taste my raindrops, k boo?
Now what you're willing, what you wanting, what you may do
Completely separated til I deeply penetrate it
Then I take it out and wipe it off
Eat it, ate it , love it, hate it
Overstated, underrated, everywhere I been
Can you wiggle wiggle for the D-O-double G again?
Come on baby, turn around
You're a star, girl, take a bow
It's just one thing that's killing me
How do you fit that in them jeans?
(Snoop Dogg)
Now make it clap (wiggle wiggle wiggle) (4 Times)
Damn baby, you got a bright future behind you


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Is Tenda Equipment & Services incorrect or missing key business information? Update your Hotfrog listing today.
Contact details
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Hotfrog Singapore provides information regarding Tenda Equipment & Services in Singapore. Tenda Equipment & Services is located at 51 Shipyard Cr, Singapore 627809 and provides Repair Services services. Contact them on 6366 2823.
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A dancer must practice for hundreds of hours in order to perfect her movements, much as a geneticist must create hundreds of abominations before perfecting a clone of his childhood cat. Try, try again with this Groupon.
The Deal
- $19 for eight children's latin or hip-hop dance classes (a $96 value)
- Classes valid for children 8–16 years old
- Latin sessions held on Tuesdays and Thursdays at 4:45 p.m.; hip-hop sessions held on Thursdays at 5:30 p.m.
Danceology owner and instructor Marla Silva has spent her life working to master the art of dance, whether she was studying it, competing in it, or teaching it. Marla, who is also certified in Sivananda yoga and Pilates, teaches a range of dance and fitness classes alongside her team of experienced instructors. The studio's specialty is social and ballroom dances, such as the foxtrot, salsa, and samba. In the spacious and bright ballroom, partners and solo dancers learn to mambo, tango, and step gracefully around a long series of puddles. Danceology also offers fitness classes including Pilates, yoga, and Zumba.
171 East Liberty Street
Toronto, ON M6K 3P6
+14165880111Get Directions


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Description: Carta Bella Circus Party Collection Kit includes 12 double-sided papers include dots, stars, harlequin diamonds, and stripes as well as patterns with circus theme icons like animal, balloons, train, tickets, pennant banners, popcorn boxes, "solids" and even some with title cards that can be cut apart and used individually. Also includes over 50 circus stickers. All in Red, Golden Yellow, Turquoise, Black, and White. Acid and lignin free. 12 x 12 inch.
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[playing piano]
- Well, hello.
Didn't knownobody was watching.
Kevin Hart hereat the legendary Cafe 4212.
And what am I doing, people?
Well, I'll tell youwhat I'm doing.
I've decided to take a breakfrom stand-up comedy.
I'm doing live recitals now.That's right.
Just tune infor what I do best,
which is humming.
♪ Hu--whoa!
♪ Hum, hum, hum
That's just oneof my tracks.
There's 82 trackson my album.
All one sound.
You know about humming,then you'll know about me.
Get outta here.This is "Hart of the City."


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Management Trainee
Job Description
Job Requirements:-
1. Work with other managers to plan and direct the work of the organization.
2. Help set policies.
3. Evaluate work output.
Job Responsibilities:-
1. Receive classroom instruction in subjects related to their rotational experience.
2. Attend lectures, watch guest speakers, and create projects, oral presentations, and take tests.
About Company
Ingram Micro Inc. is the leading matchmaker in the technology marketplace;ranked 69th on the FORTUNE 500, we are the largest wholesale technology distributor and a global leader in supply-chain management and mobile device lifecycle services. We operate at the intersection of thousands of vendor, reseller and retailer partners, reaching millions of businesses around the world.


text: PRODUCT DETAILS Order By Product Code Order By Product Name Order By Newest Electric Cylinders Ball Screw With ComDRIVE - Reducer/Motor Model 10T Electric Cylinder is a device that utilizes a source of power to operate a valve. This source of power can be a human being working a manual gearbox to open or close a valve, or it can be a smart electronic device with sophisticated control and measuring devices. Product Code 001C000443 Company Name Joyce Dayton Corp Country of Manufacture UOM Each SKU ID Quantity General Information Please click on Product Spec and identify the correct Ball Screw Jacks. You may send your Business Enquiry. Return Policy Click here to enlarge Image Link SELECT GroupName: SKU: Description: Price: Display Notes: Stock Quantity: Sold in Multiples of(CasePack): Minimum Order Quantity: Enter Quantity: ECB BALL SCREW Electric Cylinders ComDRIVE -Reducer/Motor Model 10T Total SKUs (32) ModelStatic CapacityScrew DiameterThread Pitch/LeadWorm Gear RatioEnd ConditionsAdditional Items DescriptionPrice ModelStatic CapacityScrew DiameterThread Pitch/LeadWorm Gear RatioEnd ConditionsAdditional Items DescriptionPrice ECBL81010 ton2.474 lead ball8:01Male Clevis/Clevis Base(4C)NASKUEach (USD $)1023DT11013625.39 ECBL81010 ton2.474 lead ball8:01Male Clevis/Flange Base(4F)NASKUEach (USD $)1023DT11023625.39 ECBL81010 ton2.474 lead ball8:01Male Clevis/ Rot.Clevis Base(4R)NASKUEach (USD $)1023DT11033625.39 ECBL241010 ton2.474 lead ball24:01Male Clevis/Clevis Base(4C)NASKUEach (USD $)1023DT11043625.39 ECBL241010 ton2.474 lead ball24:01Male Clevis/Flange Base(4F)NASKUEach (USD $)1023DT11053625.39 ECBL241010 ton2.474 lead ball24:01Male Clevis/ Rot.Clevis Base(4R)NASKUEach (USD $)1023DT11063625.39 ECBM81010 ton1.51.0 lead ball8:01Male Clevis/Clevis Base(4C)NASKUEach (USD $)1023DT11073625.39 ECBM81010 ton1.51.0 lead ball8:01Male Clevis/Flange Base(4F)NASKUEach (USD $)1023DT11083625.39 ECBM81010 ton1.51.0 lead ball8:01Male Clevis/ Rot.Clevis Base(4R)NASKUEach (USD $)1023DT11093625.39 ECBM241010 ton1.51.0 lead ball24:01Male Clevis/Clevis Base(4C)NASKUEach (USD $)1023DT11103625.39 Product DescriptionElectric Cylinder Specification Electric Cylinder has an input shaft (worm) shaft rotates a worm gear, which in turn rotates the lifting screw. As the lifting screw rotates, it forces the lifting nut (fixed to the cylinder tube) to translate, thus extending or retracting the cylinder tube. For proper operation, the load being lifted must be restrained from rotation. Electric Cylinders in several models including: ComDRIVE -Reducer


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Weekend Price Drops: Art House Edition12 Jan 2013 0
The list of finalists for awards at the 2013 Independent Games Festival came out this week, significantly winnowed down from the 589 games on October's longlist. There's not quite as many mobile games on the list of potential laureates as I would have expected, but iOS is still pretty well represented. There's not a single Android game amongst the finalists.
With two unavoidable exceptions, all of the iOS finalists are on sale at the moment - Spaceteam isn't on sale because it can't possibly get any cheaper (being free to begin with) and Simogo's spooky Year Walk hasn't been released yet.
Remarkable physics simulation Liquid Sketch is free at the moment, down from $0.99. The iPad-only Liquid Sketch is on the shortlist for the Technical Exellence award - you'll see why from the moment you boot it up. I've never seen a more convincing simulation of fluid motion. Well, I did once. But I probably shouldn't tell that story.
Terry Cavanagh's Super Hexagon was one of the most talked-about iOS games of 2012, but I still haven't played it. I've heard raves from friends but it looks more like an enhanced interrogation technique than a game to me. If you're possessed of a sturdy enough stomach, the Excellence in Design finalist been on sale for a few weeks now at $0.99, down from $2.99.
Excellence in Audio finalist Bad Hotel bills itself as a tower defense game - but don't hold that against it. It's kind of a defense game but it's also a musical toy and is generally much, much weirder than any other game that wears the TD epithet. Bad Hotel is on a half-price sale at $0.99.
I talked about science fiction-themed shouting facilitator Spaceteam last week - it's free, as ever. Everyone should have this game. It's on the shortlist for the IGF's Nuovo award for innovation and if it doesn't win I'll be the first one passing out the pitchforks and torches.
Log in to join the discussion.


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New Delhi, Jan 21 (IBNS) External Affairs Minister Salman Khurshid held bilateral talks with Australia Minister of Foreign Affairs Bob Carr here on Monday.
At the Joint Media Interaction with Carr, Khurshid said: "We have reviewed the entire range of our bilateral relations as well as regional and global issues. We are pleased that our Strategic Partnership has grown in strength and has expanded in scope."
"We also recognised that there are tremendous opportunities for much greater cooperation and that we shall strive to attain them.


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A Perfect Circle
A Perfect Circle's eMOTIVe is the angriest chill-out album since Nine Inch Nails' The Downward Spiral. Inspired by current events, this side project from Tool front man Maynard James Keenan covers classic songs of uprising, greed and protest, and in a detached manner that's mostly a welcome departure. Black Flag's "Gimmie Gimmie Gimmie," one of the liveliest tracks, is a music-box waltz accompanied by Keenan's froth-spewing snarl. "(What's So Funny 'Bout) Peace, Love, and Understanding," meanwhile, written by Nick Lowe and made famous by Elvis Costello, here is downgraded from an indictment to a rhetorical question, as is Marvin Gaye's "What's Going On." Fear's "Let's Have a War" gets closer to the heart of the matter, hinting at a military-industrial complex and expendable demographics.
Subversive typography notwithstanding, eMOTIVe creates an ominous mood of menacing quiet, combining string arrangements, electro beats and piano loops into a coherent downtempo disc. Though many of the tracks are unplugged, this is A Perfect Circle's most electric effort to date.
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Remember that there is more to the TCO stats than comparing how many cheap tablets will break versus the premium sticker price for rugged tablets. Organizational and productivity costs dwarf the initial device purchase price every time.... Continued
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Although DistribuTECH has always been about optimizing power generation, transmission and distribution, this year was all about optimizing the industry's utilization of "information." Utilities are becoming masters of data collection thanks to their years-long effort to implement "smart" technologies. But the resulting prevalence of data is posing new challenges. ... Continued
A high-quality workforce is a tremendous asset to any Utility. And utility technicians, across the board, have a lot of experience and skill. The challenge to utilities is that this "asset" is older than the average workforce currently driving other U.S. industries. ... Continued
When comparing eBYOD (employee) against cBYOD (customer), it's clear that they have very different applicability and implications for Utilities. cBYOD may be a smart customer engagement strategy, but eBYOD is not a smart mobile workforce strategy for Utilities – especially those worried about keeping operating costs low, increasing hardware ROI and using mobility solutions as a revenue-generating stream at the point-of-service.
... Continued
To take advantage of the Big Data model, they're going to require devices that are mobile and connected to reliable communications networks and to equipment, most likely via RFID, barcode, serial ports, etc.... Continued
Simply put, "Big Data" refers to the huge data sets that can be collected today. With mass computerization of so many aspects of our personal and professional lives, vast amounts of data can be collected and analyzed to the benefit of nearly every company.... Continued
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