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Stephens Investment Management Group Llc increased its stake in Euronet Worldwide Inc (EEFT) by 23.16% based on its latest 2016Q3 regulatory filing with the SEC. Stephens Investment Management Group Llc bought 127,132 shares as the company's stock rose 16.42% with the market. The institutional investor held 676,075 shares of the finance company at the end of 2016Q3, valued at $55.32 million, up from 548,943 at the end of the previous reported quarter. Stephens Investment Management Group Llc who had been investing in Euronet Worldwide Inc for a number of months, seems to be bullish on the $3.75B market cap company. The stock is down 0.47% or $0.34 hitting $72.69, despite the positive news. About 63,955 shares traded hands. Euronet Worldwide, Inc. (NASDAQ:EEFT) has declined 9.98% since June 7, 2016 and is downtrending. It has underperformed by 17.41% the S&P500.
Stephens Investment Management Group Llc, which manages about $3.08 billion and $3.30 billion US Long portfolio, decreased its stake in Buffalo Wild Wings Inc (NASDAQ:BWLD) by 74,080 shares to 190,712 shares, valued at $26.84M in 2016Q3, according to the filing. It also reduced its holding in Dexcom Inc (NASDAQ:DXCM) by 66,704 shares in the quarter, leaving it with 18,117 shares, and cut its stake in Taser Intl Inc (NASDAQ:TASR).
Stephens Investment Management Group Llc is a Texas-based institutional investor with more than $3.08 billion AUM in October, 2014. Taken from Stephens Investment Management Group latest Adv, the fund reported to have 15 full and part-time employees. Among which 5 performing investment advisory and research functions. The institutional investor had between 26-100 clients.
Insitutional Activity: The institutional sentiment decreased to 0.89 in 2016 Q3. Its down 0.28, from 1.17 in 2016Q2. The ratio is negative, as 35 funds sold all EEFT shares owned while 96 reduced positions. 36 funds bought stakes while 81 increased positions. They now own 46.17 million shares or 1.22% more from 45.61 million shares in 2016Q2. Moreover, Renaissance Technologies Lc has 0.11% invested in Euronet Worldwide, Inc. (NASDAQ:EEFT) for 761,045 shares. Teacher Retirement Systems Of Texas holds 0% of its portfolio in Euronet Worldwide, Inc. (NASDAQ:EEFT) for 5,305 shares. Raymond James Financial Svcs Advsrs holds 3,052 shares or 0% of its portfolio. Jupiter Asset Limited has 150,450 shares for 0.46% of their US portfolio. Moreover, Nicholas Investment Ptnrs LP has 0.32% invested in Euronet Worldwide, Inc. (NASDAQ:EEFT) for 58,445 shares. Eaton Vance last reported 0.04% of its portfolio in the stock. Vanguard Group has 0.02% invested in the company for 3.59 million shares. Ameriprise Inc has 0.02% invested in the company for 462,865 shares. First Republic Investment Mgmt last reported 0% of its portfolio in the stock. Panagora Asset Management last reported 0% of its portfolio in the stock. Ny State Common Retirement Fund has 0.02% invested in the company for 189,747 shares. Portolan Capital Mngmt Lc holds 2.74% of its portfolio in Euronet Worldwide, Inc. (NASDAQ:EEFT) for 244,271 shares. The North Carolina-based Bb&T Corp has invested 0.02% in Euronet Worldwide, Inc. (NASDAQ:EEFT). Crow Point Prtn Lc has 886 shares for 0.01% of their US portfolio. Paradigm Asset Mngmt Llc owns 1,400 shares or 0% of their US portfolio.
Insider Transactions: Since August 3, 2016, the stock had 0 buys, and 5 sales for $5.69 million net activity. On Tuesday, August 16 the insider NEWMAN JEFFREY B sold $449,062. Another trade for 58,573 shares valued at $4.42M was sold by Caponecchi Kevin J. Fountas Nikos sold $21,747 worth of stock or 296 shares.
More notable recent Euronet Worldwide, Inc. (NASDAQ:EEFT) news were published by: Fool.com which released: "Why Euronet Worldwide, Inc. Plunged Today" on February 10, 2016, also Fool.com with their article: "Higher Operating Margin Boosts Euronet Worldwide's Earnings" published on October 25, 2016, Fool.com published: "Euronet Worldwide Inc.: Money Transfer Business Charges Ahead" on October 26, 2015. More interesting news about Euronet Worldwide, Inc. (NASDAQ:EEFT) were released by: Fool.com and their article: "Euronet Worldwide Capitalizes on Transaction Demand" published on July 26, 2016 as well as Fool.com's news article titled: "Investing in Socially Responsible Companies: Euronet Worldwide, Inc." with publication date: November 08, 2014.
According to Zacks Investment Research, "Euronet Services Inc. is an industry leader in providing secure electronic financial transaction solutions. The company offers financial payment middleware, financial network gateways, outsourcing and consulting services to financial institutions and mobile operators. (Press Release)"
Euronet Worldwide, Inc. (NASDAQ:EEFT) Ratings Coverage
Out of 7 analysts covering Euronet Worldwide (NASDAQ:EEFT), 6 rate it a "Buy", 0 "Sell", while 1 "Hold". This means 86% are positive. Euronet Worldwide has been the topic of 11 analyst reports since July 21, 2015 according to StockzIntelligence Inc. The stock has "Market Perform" rating given by Avondale on Thursday, February 11. The company was upgraded on Wednesday, December 23 by Monness Crespi & Hardt. The firm earned "Buy" rating on Tuesday, July 21 by Evercore. Monness Crespi downgraded the stock to "Neutral" rating in Friday, June 10 report. The stock has "Overweight" rating given by Piper Jaffray on Wednesday, November 25. SunTrust initiated the shares of EEFT in a report on Wednesday, October 12 with "Buy" rating. As per Friday, July 31, the company rating was downgraded by Monness Crespi & Hardt. As per Wednesday, September 2, the company rating was maintained by Piper Jaffray. On Wednesday, January 20 the stock rating was initiated by Northland Capital with "Outperform". The firm earned "Market Outperform" rating on Wednesday, April 27 by Avondale.
EEFT Company Profile
Euronet Worldwide, Inc. (Euronet), incorporated on December 13, 1996, is an electronic payments provider. The Firm offers payment and transaction processing and distribution solutions to financial institutions, retailers, service providers and individual consumers. The Company's product offerings include automated teller machine (ATM), point-of-sale (POS), card outsourcing, card issuing and merchant acquiring services; software solutions; electronic distribution of prepaid mobile airtime and other electronic payment products, and global money transfer services. The Firm operates in three divisions: EFT Processing Segment, which provides electronic payment solutions consisting of ATM cash withdrawal and deposit services, ATM network participation, outsourced ATM and POS management solutions, credit and debit card outsourcing, and card issuing and merchant acquiring services; epay Segment, which provides electronic distribution and processing of prepaid mobile airtime and other electronic payment products and collection services for various payment products, cards and services, and Money Transfer Segment, which provides global consumer-to-consumer money transfer services under the brand names Ria and AFEX Money Express and global account-to-account money transfer services under the brand name HiFX.
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Discussion in 'Tag Heuer Replicas' started by koster88, Aug 11, 2008.
they do make a newer version of this watch that has Calibre 17 like the orig and with lume.
Yes recently got one, with the calibre 17 on rotor.. but the lume seems to be very minimal... or my eyes are playing a trick on me... maybe mine still does not have a lume
Where was the watch purchased from>? Been looking for a nice one for awhile... too hesitant to purchase from most sites!
Thank You.
could anyone please let me know where i can buy one of these? also I would like to know the prices on these rep, thanks
Welcome strade, all our trusted sellers have this rep just ask
I have the RS pvd version. I like it but the strap and buckle are quite poor, I have replaced both
And its not a limited edition
Did you replace your buckle with Gen ones? I just received the SS version and the buckle doesn't do the watch justice at all...
No, I have a "normal" buckle for the moment, I will try to find one (if its cheap...)
Sorry for the noob question - where did you purchase the watch?
awesome, aweosme review. I have always loved the Carerra's and is one of my favorite watches. Thanks again for your review. I am thinking about picking up a gen carerra 43mm. Also just picked up my wife a gen Aquaracer from Alan Furman.
+1 on a good review. I have a black PVD one on the way now. Thanks!
Can anyone recommend a dealer who does a Good quality rep with good movement of this? The watch looks very nice
try Hont or MReplica.
Hont does everything and MReplica can definately get this for you. he will QC it for you personally as well.
i all
Very nice review, i have one in brow leather strap, but is also more bad than your (21j), but i love it
Where did you buy this watch?
Koster, thanks for your post. Really interesting! Cand you send me PM with the web where you buy the watch? Thank you!
Very nice review and waiting next review for Tag cal36
I heard that modified A7750 movement is a bit problematic as they moved sec@9 to sec@3.
Great post, thanks
hi koster. which td did u use
Hi koster
im a newbie. could You let me know the TD you got it from and the price. (can i ask this??) many thanks


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Hotfrog Ireland provides information regarding Blackbird in Ballycotton. Blackbird is located at Main St, Ballycotton and provides Social services. Contact them on (021) 464 7884 or by visiting them on their website http://www.blackbirdballycotton.com.
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This fall, Broward County, Fla., schools may use a special aerosol spray to detect traces of illegal drugs on backpacks, lockers and other surfaces. While the ACLU worries about eroding student rights and false positives, school officials say they would use the residue-detecting spray only in cases of probable cause.
Full Story:The Miami Herald (tiered subscription model)


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Livonia Public Schools is searching for its 2017-2018 Teachers of the Year, for the elementary and secondary levels.A committee of teachers, administrators, and residents has formed to select the designees, to be annouced in mid-May or early June.
Nominees must possess exemplary personal qualifications, professional skills, and records of school/community service. In addition, they should spend at least half of their time teaching in a regular classroom, and have tenure status.
All areas must be completed for nomination to be considered by the committee. Please write in complete sentences, giving specific details and/or examples where applicable.
Teacher Name
Grade or Subject
In narrative form, give brief examples of the following:
Personal characteristics of the nominee (integrity, persistence, caring, etc.
Excellence in and commitment to the teaching profession (events, quotes, innovations, successes, good personal relationship with students and teachers, etc.)
Related professional experience (participation in efforts to improve the education and social conditions of the community, etc.)
Your name
Your phone number
Your email address
Relation to nominee
Questions regarding the Teacher of the Year program may be directed to Stacy Jenkins at firstname.lastname@example.com. All nominations must be submitted by Tuesday, February 28.


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No, Not Our Type of "Lean"
This ad in the Minneapolis/St. Paul airport caught my eye today (sorry for the blur, I was on a moving walkway…)
This is probably not to be confused with law firms that are using Lean principles (as I've written about before).
Subscribe via RSS | Lean Blog Main Page | Podcast | Twitter @leanblog
Thanks for reading! I'd love to hear your thoughts. Please scroll down to post a comment. Click here to be notified about posts via email. Learn more about Mark Graban's speaking, writing, and consulting.


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best price golf clubs
The Ping G25 driver has a 460cc head with a larger profile design. The ultra-thin-crown and aerodynamic design redistributes weight along the perimeter of the clubhead, moving the CG (centre of gravity) farther back and lower than any previous Ping driver. This new CG position increases the clubs MOI (moment of interia) and makes it extremely forgiving.
Ping engineers made structural reinforcements to the crown, sole and skirt in order to offer the G25 a solid feel and sound. Variable thickness behind the face powerfully transfers the energy of a players swing into the ball, producing fast ball speeds leading to greater distances off the tee.
Ping have developed the innovative TFC 189D shaft specifically for the G25 driver. This lightweight, high-balance point shaft has a new non-glare, charcoal finish to help eliminate reflections, improve alignment and inspire confidence at address.
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The TaylorMade R11S Driver is further enchanced by TaylorMade's Inverted Cone Technology that expands the COR zone, or the portion
Titleist has always been about tradition and seem to always be going after the better player. The new AP2 712
The i20s offered a consistent and accurate flight, with a piercing trajectory. If precision and accuracy are important in your
Callaway has reached a new level of performance in a forged iron, offering avid players the chance to play with
TaylorMade RocketBallZ RBZ Driver is expected to hit the market very soon. People interested in golf can enjoy better playing
The TaylorMade RocketBladez iron has been launched with much fanfare, but that is OK when there is a significant technical
The RocketBallz RBZ Stage 2 Driver has an expanded clubface that gives the golfer substantially more face area. This driver
RocketBallz Stage 2 Fairways are engineered to be extremely playable and easy to launch, thanks to the clubhead's lower profile,
The Ping G25 irons are high-launching, game-improvement implements with a dark, non-glare matte finish. They have a re-engineered Custom Tuning
Like the R11 driver, the first thing you'll notice about the SuperFast 2.0 is its white crown. By designing a
The R11 irons are inspired by the R11 driver series and have integrated some of the design features of the
The full body Diamond Muscle design of the Grain Flow Forged® MP-64™ irons delivers increased forgiveness in the long irons
Both R1 fairway wood and driver apparently will feature an all-lofts-in-one-head design similar to R11 and R11s driver. TaylorMade may
The Ping G25 Fairway Wood, their highest MOI fairway wood, features a slightly larger head design with a traditional shape
The TaylorMade R1 Driver can be tuned to fit tour pros and amateurs alike. It can be tuned to any
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Bundle and save with Tracktion's Ultimate package, providing all the tools needed to create your next masterpiece.
NOTE: Please check the Celemony website for detailed system requirements for their Melodyne Software
Check out the following pages for more information on the Tracktion software products:
Existing customers click here for upgrade version.
|Current Version
|File Type
|Supported OS
|Mac 32bit, Mac 64bit, PC 32bit, PC 64bit, Linux


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You are currently using an unsupported web browser.
We support the latest versions of these browsers:
If you have any problems while attempting to download a browser, you should contact your organization's system administrator.
For any questions related to Cvent, feel free to contact our Customer Care team.
AAA Rating is a rating system for lodgings and restaurants in North America by American Automobile Association.
This rating was reported by the venue and has not been verified by Cvent.
Forbes Travel Guide is formerly known as Mobil Travel Guide. These rating apply to hotels, restaurants, and spas around the world.
Northstar Ratings are powered by Northstar Travel Media and are based on objective and unbiased evaluations from experienced editors that individually analyze, research, and evaluate hotels. Hotels and suppliers are not required to pay any fees or pay for memberships to have their property rated.
Northstar Ratings are powered by Northstar Travel Media. Please email email@example.com if you have any questions or comments regarding the rating of an individual property.
Drury Hotels has been recognized 11 years in a row by J.D. Power with the award for 'Highest in Guest Satisfaction among Mid-Scale Hotel Chains'
Group cancellation less than 30 days prior to arrival or failure to comply with attrition clause results in cancellation fee being calculated as a percentage of lost revenue. Individual cancellation is 12pm on the day of arrival.
You must be logged in to view contact information for this supplier. Sign up for a free Cvent account today.
Check-in time: 3:00 pm
Check-out time: 11:00 am
Pets Accepted
Rooms with pets will be charged a daily cleaning fee of $25 per room plus tax. Service animals are welcome free of charge. Limit of two pets per room.
Parking: $16.00/night
(may be higher during special events)
*Parking garage clearance is 6'6". Cargo and 15-passenger vans will not fit in garage.
For guests who are not staying overnight, there are several parking garages located within 1 block of the hotel.
Tour Buses must have a permit from the city to park. Please contact 210-207-7755 for bus parking options.
Located steps away from Rivercenter; our hotel is in a prime area of the Riverwalk. We are one of the only hotels in the area to offer a complimentary hot breakfast each morning and evening reception featuring hot items and cold drinks! Texas Land and Cattle Steakhouse is conveniently located on our Riverwalk level.
Admire the 1920's architecture of our historic building, offering a unique venue for San Antonio meetings and celebrations. With customized menus, a devoted events team and 4,000 sq. ft. of function space let us help make your dream event a reality.
The Homewood Suites by Hilton San Antonio North hotel in Texas is located in the beautiful Stone Oak suburb of San Antonio, Texas close to numerous office buildings, medical facilities, well-appointed shopping areas & adjacent to Cornerstone Church.


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Product DescriptionTefal Aquaspeed Iron FV5540 Blue
- Cable Length 200 cm
- D 11 cm
- 1 year guarantee
- 1 heat setting
- H 15 cm
- Plastic
- Tefal`s Smart Protect FV4970 lets you iron with total ease. No setting is needed, just switch on and go! Thanks to its Smart Technology there`s no need to set the iron and no risk of burning clothes. Smart Technology sets the optimal combination of temperature and steam so not only does it remove creases fast, it is also safe to use on all ironable fabrics. You can iron both tough or delicate fabrics without burning. For total peace of mind this iron also features a smart Auto-off function. 150 g/min shot of steam helps remove tough creases and the Durilium Technology soleplate offers outstanding glide, optimal steam distribution and excellent scratch resistance. This soleplate also features a precision tip for detailed ironing. Iron for longer thanks for the large 270ml water tank. Refilling is fast and easy thanks to the extra large water tank opening.
- 1 Program
- Steam boost
- Steam Rate 150 grams
- Irons
- Vertical steam
- Water Tank Capacity 2.7 L
- W 31 cm
- Weight 1.3 kg
- Depth 30 cm
- Height 18 cm
- Width 14 cm
Buy and Collect - FREE for orders over £30 or £2 for under £30 (7 days a week)
Order before midnight for collection in store from 12 noon the NEXT DAY or on a day of your choice
(For Belfast and Dundrum please allow up to 48hrs for delivery.)
Standard - £3.95 (Monday to Friday)
FREE on orders of £50 or more. Delivery within 3 - 5 working days.
Next Day - £6 (Monday to Sunday)
Order before 7pm for delivery the NEXT DAY
(For Belfast and Dundrum please allow 48hrs for delivery.)
Nominated Day - £6 (Monday to Sunday 6pm)
Same Evening - £8 (Monday to Friday)
Selected postcodes only. Order by 12noon for delivery between 6-9pm.
Ireland - £3.95
FREE on orders of £50 or more*. Delivery within 3 - 5 working days.
Find out more about delivery and charges
We hope you will love your new things but if you don't, you are welcome to return your items free of charge. Here's how to do it:
To a Store
Bring your items and proof of purchase into store within 28 days from the day your parcel arrives, for a full refund via the original payment method.
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Please send your return by post or courier within 28 days from the day your parcel arrives for a full refund via the original payment method.
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You will need to return your goods by post, if PayPal was your original payment method and you would like the refund back to your account. If you make your return to a store, we will only able to give you a House of Fraser gift card to the same value. You'll still have 28 days to return.


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Edwards Lifesciences www.edwards.com
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Cqb G Series Hight Temperature Electromagnetic Chemical Pump - Chinaprice: contact company for price
cooling structure to ensure the dependability and stability of prime
mover and magnetic drive. Meanwhile, pin coupling is used to reduce the
noise and vibration of pump. The detachable type and pin coupling makes
the structure of pump longer, which is good for heat dissipation.
Moreover, to make repair and replacement of spare parts much easier, a
heat dissipation fan blade is designed on the outer rotor of pump to
ensure the stability of magnet steel. This series of pumps are
applicable for high temperature mediums, with service temperature is not
higher than 300 degree celsius.
Company Contact:
- Posted By: Kenshine Pump And Valve MFG Co.,LTD
- Phone: 862133592713
- Address: Yindu Road 1535,Minhang District, Shanghai, Shanghai, China
- Email:
- Website: http://www.kenshine-pump.com
Published date: January 15, 2015 -
- Business Description: Manufacturer and sales for various pumps such as centrifugal
pump,industrial pump,chemical pump,submersible pump,self priming
pump,diaphragm pump,electromagnetic pump, gear pump,multistage pump etc
KENSHINE PUMP&VALVE MANUFACTURING CO.,LTD is the integrated enterprise specializing in research and development and design and produce and sales of pump and valves. KENSHINE PUMP&VALVE MANUFACTURING CO.,LTD locates in MINHANG industrial zone, SHANGHAI which is the economic trade center in China and established in 1985. KENSHINE PUMP&VALVE MANUFACTURING CO.,LTD have developed and enlarged many years, supported by scientific management,sophisticated technology,advanced equipment and strict quality system. Company is continuously going up as the 40% rising speed every year and is as the one of industrial manufacturer in China. Company had ISO certificate in 1998 and the products are according to JB/JQ20103 1998 and JB/JQ20104 1998 standard. The quality of products is insured by PICC.
Company has several sales and service networks in many middle and big cities of China. The products are sold to domestic cities and exported oversea about 40% products.
Related listings
Ihf Fluoroplastic Chemical Industrial PumpPumps - Kenshine Pump And Valve MFG Co.,LTD - China - January 15, 2015 - contact company for price
IHF Series single stage single suction fluoroplastic alloy centrifugal pump in chemical industry is manufactured as international design and principle of nonmetal pump. The pump has the advantages such as anticorrosion and wearable and anti- high tem...
Sell S Stainless Steel Submersible Sewage PumpPumps - Kenshine Pump And Valve MFG Co.,LTD - China - January 15, 2015 - contact company for price
Summary S Series stainless steel submersible sewage pump is suitable to transfer waste water with acid and alkali component such as sewage treatment in pharmaceutical factory, chemical plant, food factory and seawater treatment etc. The whole pump ca...
Sewage Pump Submersible WaterPumps - Tianjin GanQuan Group Corporation - China - December 8, 2014 - contact company for price
Discharge: 5 to 6000 m3/ h ( 1.4- 1666.7l/ s) Lift Head: 6 to 80 m ( 19.7- 262.5 feet) Max. rotary speed: 2900 r/ min Pump outlet diameter: 50- 600mm ( 2- 23.6 inch) Temperature: - 5 to +50 deg. C Maximum system pressure: <1 Mpa PH value: 2 to 13 ...
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text: Jesus is a jerk. Click to block a category:GamingPoliticsNewsComicsAnimeOther pastey next random Comment Top NextComment Silly Jesus, your a ghost, not a human, go back to heaven. next Tags: Jesus | is | a | Dick Omelete System 32, it' ll make your computer run faster." ... +14 Views: 2674 Submitted: 12/19/2010 Facebook Twitter Google+ Tumblr Pinterest


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Breaking Skin Tattoo & Body Piercing Studio, tattoo shop, listed under "Tattoo Shops" category, is located at 54917 National Rd Bridgeport OH, 43912 and can be reached by 7407380746 phone number. Breaking Skin Tattoo & Body Piercing Studio has currently 0 reviews.This business profile is not yet claimed, and if you are the owner, claim your business profile for free. If you are not the owner you can invite this business to join by email or by phone.Browse all Tattoo Shops in Bridgeport OH. Discover census data for Bridgeport, OH.
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What is the Workshop?
The Workshop is our crowd funding platform for new products.
How does it work?
At the beginning of each month, we release a newly designed collection in the Workshop. For an entire month these products are available to pre-order at a discounted price. By purchasing these products before they enter production, you as our customer assist us in funding the production process. Products that reach their funding goal are manufactured and delivered. At the close of each campaign, customers will be notified of what they can expect next in return for their participation.
Why should I participate?
By participating in the Workshop, you get to help bring Taylor Stitch designs to life. You also get to lock in pre-sale discounts that average more than 20%. There is the chance that products sell out in the crowd fund period as well, so participating can guarantee that you get your size.
How long is the funding period?
A Workshop collection's funding campaign begins at the beginning of a month and ends on the last day of the month.
How long does it take to deliver products?
With every Workshop product, we include an estimated shipping date. Generally this is between eight and ten weeks. More complex garments can take longer. While it's not a common occurrence, difficulties in manufacturing can arise and in those cases, we'll be in touch as soon as possible to update you if our original projection changes.
When am I actually billed?
We'll charge your card upon checkout.
What happens if a product doesn't reach its goal?
In the event a Workshop product does not reach its funding goal, we'll be in touch to let you know if we decide to make the products anyhow or to provide you a full refund.
Can I just wait for the product to land at retail?
Of course. You just won't be able to lock in pre-sale pricing and there is a chance it may sell out.


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Product added to your basket
Mens Fremen Boots
rrp £119.99
Mens Fremen Boots
rrp £119.99
Product added to your basket
Product code: 33818
Colour: Black
Tell a friend Add to wishlist
Tell a friend
Lacoste men's Fremen boots in black.
These workman style boots from famous label Lacoste will never be off your feet. We won't blame you either!
With a thick washed black leather upper and fleecy lining, they're just what you need for colder weather. They have four lace up eyelets and two hook and eye fastenings at the top. The sole has three colour stripes and contrasts against the brown leather detailing.
The famous crocodile logo that everyone knows Lacoste for is prominently displayed on the outside of each boot.
These boots have leather uppers, leather and textile linings and a synthetic sole.
Delivery info
Standard Delivery £3.95 (2-5 days)
Next Day Delivery £5.95
(see terms and conditions for full details) View Full Delivery Policy
Returns info
If you are unhappy with your purchase for any reason, return it to us (unworn) within 21 days and we'll give you a full refund or exchange (see terms and conditions for full details)
View Full Returns Policy
Get the most out of returns with GetTheLabel... shop our pre-paid returns labels here: Click here for Royal Mail or Click here for Collect+


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North & South Builders
builders, Residential Real Estate Listings, home construction
North & South Builders was built on the ethos of customer satisfaction. Our aim is to provide a premium service without the premium cost. All of our work is tailored to your unique and specific requirements. We pride ourselves on our ability to offer the highest quality of workmanship and we're willing to put that under the spotlight with our one year workmanship guarantee. Don't hesitate to get in touch. We specialise in full home renovations, turning your dreams into a reality. North and South Builders can even help in the design process, whether that's arranging for your kitchen to be designed exactly how you want it or providing bathroom ideas tailored to suit you. Our quality control and standards are always on the rise, which means you can be positive that we're striving to improve on every single job we undertake. That's a cast iron guarantee from the team here at North & South Builders. We specialise in the design and installation of kitchens and bathrooms. We only use the best available products, making sure that the finished item has the "WOW" factor that you're looking for. When redesigning a kitchen or bathroom, you may decide that you want new tiles installed or the room freshened up with a lick of paint. Either way we have you covered. North & South Builders have some of the best tradesmen available. Why move home when you can transform your existing property to suit your needs, all whilst adding value? Renovating your home or adding an extension can help you to achieve this. You can have more space to raise a family, perhaps a larger office for your growing business or simply a new, more modern look. Whatever your needs, we are here to help.
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Michael Henderson is a sport writer and contributor to the Guardian
If Wayne Rooney wants to be a true great he should look to Paul Scholes for a lesson in humility, writes Michael Henderson.
This morning, the London Symphony Orchestra's new star will direct a celebration of Mozart's birth in Vienna. It's quite an achievement - but then this 30-year-old Briton is one of the most dynamic men in music today.


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A little bit about Me
My name is Jenn I am 33 Writing is my one true passion also family is a big part of who I am and there to love and support me in all that I do I thank god for them everyday because I don't know what I would do without them.
My Favorite Movies
Twilight New Moon Eclipse Breaking Dawn 1 & 2 Divergent Insurgent The Last Song The P.O.T.C Trilogy The first and third movies are AWESOME the second one kinda freaked me out A Cinderella Story John Tucker Must Die The Fantastic Four The Fantastic Four Rise Of The Silver Surfer and many more
My Favorite TV Shows
Hawaii Five 0 NCIS NCIS Los Angeles NCIS New Orleans Blue Bloods Bones
My Favorite Book/Movie couples
Twilight Saga Edward & Bella Jasper & Bella Emmett & Bella
Jake & Bella Seth & Bella Paul & Bella Jared & Bella Quil & Bella Embry & Bella Sam & Bella
Divergent Tris & Four
The Fault In Our Stars Gus & Hazel
My Favorite TV Couples
Bones FBI Special Agent Seeley Booth & Dr.Temperance " Bones" Brennan
NCIS Tony and Ziva
NCIS LA Kensi & Deeks
My Favorite Books
New Moon
Breaking Dawn
The Fault In Our Stars
The Cullens
Edward Cullen: Sexier Than You since 1918
Alice Cullen: Quirkier than You since 1901
Jasper Hale: Charming Ladies since 1864
Emmett Cullen: Stronger Than You since 1935
Rosalie Hale: Better Than You since 1916
I love to write oh and did I mention that I LOVE to SPOIL my cousins. I love sports & Music
Football and Baseball are my two favorites my favorite teams are the NY football Giants and The NY Yankees
I love all kinds of Music


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For B2C Marketing Professionals
Forrester Research World Online Population Forecast, 2012 To 2017 (Global)
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August 13, 2012
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The world online population forecast estimates the number of individuals with online access and includes total population and Internet penetration by region and country. This updated model includes both PC online and mobile Internet users in the online population. Includes data for five regions and 56 countries. Includes historical data going back to 2005 plus a five-year forecast from 2012 to 2017.
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Governance, Board Succession & Remuneration Committee Meeting Minutes
20 August 2012, 2.00m
Kim Heitman (Chair), Paul Levins, Julie Hammer, Cheryl Langdon-Orr, Marty Drill, Chris Disspain, Jo Lim
Staff Support:
Jacki O'Sullivan (Minutes)
1. DBCDE Draft ccTLD Governance Protocols
• A draft response to the letter received by Keith Besgrove, DBCDE was circulated for Committee review and the Chair invited the Committee to discuss and provide feedback to the draft response.
• The Committee discussed possible issues, an agreed approach and response.
• The Committee agreed:
- auDA's main objective is to maintain the security and stability of .au.
- C Disspain would advise Keith Besgrove that a formal response would be sent.
- J Lim & P Levins to prepare the draft response and address the main points. The response will be sent to the Committee email list with a special Governance meeting set if further discussions were required.
- once finalised, the draft response would be sent to the closed Board list for review and approval.
- Committee agreed any formal Letter of Endorsement should be signed by the Minister consistent with the original Letter of Endorsement.
- aim for final response completion is end September 2012.
2. CONFIDENTIAL: CEO's Remuneration:
Closed item. Observers left the meeting.
3. Any other Business:
Julie Hammer suggested the list of various auDA Committees and number of meetings held should be listed in the Annual Report. The Committee agreed.
4. Next meeting
The Committee confirmed the next meeting will be held on Tuesday 2 October 2012 at 12.00noon AEDST via teleconference.


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If I was in Scotland I'd now vote SNP and yes in a referendum after this election. Shown up Westminster as 'UK run by England only' culture.
Enter Boris Johnson and Nigel Farage. Lovers of the north, saviours of the working class.
Man I love @NicolaSturgeon. Imagine if we had an adult in charge of the entire UK not just Scotland #marrshow
North-east no surprise but still blowing my mind. How has the last 6 yrs of austerity worked out for you? Fancy another 10 with no EU help?
#jobs @FutureGov looking to fill right now... head of ops, head of sales, head of marketing, designers. Fancy it? Know anyone? Let me know!


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Karyopherinβ2 (Kapβ2) or transportin imports numerous RNA binding proteins into the nucleus. Kapβ2 binds substrates in the cytoplasm and targets them through the nuclear pore complex, where RanGTP dissociates them in the nucleus. Here we report the 3.0Å crystal structure of unliganded Kapβ2, which consists of a superhelix of 20 HEAT repeats. Together with previously reported structures of NLS and Ran complexes, this structure provides understanding of conformational heterogeneity that accompanies ligand binding. The Kapβ2 superhelix is divided into three major segments. Two of them (HEAT repeats 9–13 and 14–18), which constitute the substrate binding site, are rigid elements that rotate relative to each other about a flexible hinge. The third (HEAT repeats 1–8), which constitutes the Ran binding site, exhibits conformational changes throughout its length. An analogous segmental architecture is also observed in Importinβ, suggesting that it is functionally significant and may be conserved in other import karyopherins.
Karyopherinβ proteins (Kapβs; importins/exportins) mediate transport of macromolecules between the nucleus and the cytoplasm. At least 19 members of this protein family have been identified in human cells, and most of them have been reported to function in nuclear import, export, or bidirectional transport (reviewed in Chook and Blobel, 2001; Conti and Izaurralde, 2001; Gorlich and Kutay, 1999; Weis, 2003). All known Kapβs consist of 19 or 20 contiguous HEAT repeats (Huntington, Elongation factor 3, A subunit of protein phosphatase 2A and Tor1 kinase, proteins where these types of helical repeats were first found; Andrade and Bork, 1995) that pack to form a spiral-shaped or superhelical molecule (Chook and Blobel, 2001; Conti et al., 2006; Cook et al., 2007). Each Kapβ recognizes multiple ligands, including numerous transport substrates, nucleoporins, and the Ran GTPase. Kapβs bind specific sets of substrates through nuclear localization or nuclear export signals (NLSs or NESs). The number of substrates for a Kapβ ranges from one to hundreds. For example, Kapα is the only known substrate for export-Kapβ Cas/Cse1p, whereas Importinβ (Impβ or Kapβ1) and Crm1 bind hundreds of substrates each (Matsuyama et al., 2006; Mosammaparast and Pemberton, 2004). Kapβs also bind FG repeats in nucleoporins to translocate substrates through the nuclear pore complex (NPC). Finally, the Ran GTPase regulates Kapβ-substrate interactions and transport directionality through its nucleotide cycle and the asymmetric distribution of its nucleotide states across the NPC. Thus, it is critical that this family of transporters maintains an architecture that facilitates binding and exchange of many diverse ligands.
Multiple crystal structures of four different full-length Kapβs and small-angle X-ray scattering (SAXS) models of six different Kapβs have been reported. Previously solved crystal structures are: (1) complexes of human Impβ with substrates Kapα IBB domain and SREBP2 (Cingolani et al., 1999; Lee et al., 2003); (2) complexes of yeast homolog Kap95p with RanGTP and a Nup1p fragment (Lee et al., 2005; Liu and Stewart, 2005); (3) human Kapβ2 or transportin complexed with RanGppNHp, substrates hnRNP A1-NLS and hnRNP M-NLS, respectively (Cansizoglu et al., 2007; Chook and Blobel, 1999; Lee et al., 2006); and (4) unliganded and substrate-bound Cse1p (Cook et al., 2005; Matsuura and Stewart, 2004). SAXS models are available for multiple states of Impβ, Kapβ2, Crm1, Cse1p, and Xpo-t and its yeast homolog Los1p, and comparisons within each karyopherin series show substantial protein flexibility (Fukuhara et al., 2004). However, low-resolution SAXS models limit characterization of flexibility to large-scale changes in radius of gyration and descriptions of extended versus compact conformations (Fukuhara et al., 2004). Crystal structures of Cse1p (Cook et al., 2005; Matsuura and Stewart, 2004) and full-length Impβ complexes (Cingolani et al., 1999; Lee et al., 2003, 2005; Liu and Stewart, 2005) show that both karyopherins undergo large conformational changes upon ligand binding, also showing that Kapβs are quite flexible. However, despite the large number of crystal structures, there are relatively few studies of Kapβ conformational flexibility (Cingolani et al., 1999; Conti et al., 2006; Cook et al., 2005; Lee et al., 2003, 2005). With the exception of a recent review on Kapβ flexibility (Conti et al., 2006), most studies involve qualitative descriptions of differences in superhelical pitches and regions of structural changes. Analysis of conformational flexibility may be complicated by the unusual nonglobular and nonmodular architecture of these proteins and the limited number of structures within each Kapβ series (Impβ, Kap95p, Kapβ2, and Cse1p).
We have solved the 3.0Å structure of unliganded Kapβ2 to extend the structural map of this import pathway that transports numerous RNA binding proteins into the nucleus. A suite of Kapβ2 structures (unliganded, hnRNP A1-NLS, hnRNP M-NLS, and Ran complexes) is now available to explain conformational changes that accompany substrate recognition, Ran binding, and substrate release (Cansizoglu et al., 2007; Chook and Blobel, 1999; Lee et al., 2006). Most importantly, through three independent methods of domain motion analysis, rotation vector clustering (Hayward and Berendsen, 1998), B factor grouping (Painter and Merritt, 2006), and structural superpositions, we have identified three major segments of the Kapβ2 superhelix that show intrinsic flexibility and also respond to binding of different ligands. We also observe segmental architecture in Impβ, suggesting generality across nuclear import factors.
Kapβ2 has 20 HEAT repeats, each consisting of two anti-parallel helices, A and B. The A helix of HEAT repeat 1 (H1) is abbreviated as H1A. All helices are connected by short loops or small helices except for H8A and H8B, which are connected by a 62 residue acidic loop called the H8 loop (Lee et al., 2006). The Kapβ2 superhelix can be divided into two arches. The N-terminal arch is composed of H1–H13, and the C-terminal arch is composed of H9–H20. Ran binds in the N-terminal arch and substrates have been observed so far to bind in the C-terminal arch.
We have solved the 3.0Å crystal structure of unliganded Kapβ2 using a combination of single-wavelength anomalous dispersion (SAD) and molecular replacement (MR). Full-length native and selenomethionine Kapβ2s (residues 1–890) were crystallized. The native crystals (space group P21, a = 129.9Å, b = 169.3Å, c = 141.1Å, and β = 93.1°, four molecules in the asymmetric unit; Table 1) diffracted to 3.0Å. MR using the Kapβ2-Ran structure was unsuccessful (McCoy et al., 2005), suggesting significant conformational differences between the unliganded and Ran states.
Selenomethionine Kapβ2 crystals (space group P21, a = 108.5Å, b = 294.0Å, c = 108.5Å, and β = 92.0°, four molecules in the asymmetric unit) were pseudo-merohedrally twinned, but a 1:1 molar mixture of native and selenomethionine proteins resulted in a single crystal (space group P21, a = 108.5Å, b = 294.0Å, c = 108.3Å, and β = 92.0°) from which 3.6Å SAD data were obtained. Even though MR using Kapβ2 residues 396–864 from the Kapβ2-Ran structure was successful (McCoy et al., 2005), we calculated experimental SAD phases to avoid model bias. To facilitate determination of the 104 selenium sites in the asymmetric unit (26 methionines/Kapβ2), 72 selenium sites were extracted from the partial MR model followed by determination of the next 28 by iterative phase refinement and difference Fourier methods (Brunger et al., 1998). Phase refinement with 100 selenium sites followed by density modification with solvent flipping resulted in an electron density map where three of the four Kapβ2s in the asymmetric unit could be traced. This incomplete and low-resolution SAD model was not refined, but instead used for MR with the higher-resolution native data. Residues 88–656 gave an MR solution, resulting in an interpretable electron density map. Differences between the SAD and native models are discussed in Experimental Procedures. Given the four molecules in the asymmetric unit and the unusual helical repeat structure of Kapβ2, the final 3.0Å model of residues 6–890 for chains A, C, and D and residues 31–890 for chain B was refined using many combinations of noncrystallographic symmetry (NCS) constraints (described in Experimental Procedures; see Tables S1 and S2 in the Supplemental Data available with this article online). Statistics of a refinement using NCS to constrain chain A to D and chain B to C, both in three segments, are reported in Table 1 (Rfree = 28.6% and R factor = 26.1%) and Tables S1 and S2 (CCP4, 1994).
All four copies of Kapβ2s are arranged with their superhelical axes parallel to the crystallographic y axis, and two pairs (chains A and B, and C and D) are related to each other by a pseudo-translation of approximately a half-unit cell along the crystallographic z axis. Approximately 4700Å2, or 12% of each molecule's total surface area, is buried in crystal contacts. The low percentage of surface area involved in crystal contact compared to other protein crystals is consistent with the low resolution and high solvent content (>70%) of the Kapβ2 crystal (Carugo and Argos, 1997). All NCS-related Kapβ2s have similar crystal contacts and similar overall structure.
The unliganded Kapβ2 molecule is a rather symmetrical superhelix with a 77 Å pitch, 115 Å length, and 65 Å diameter (Figure 1). Its overall structure is similar to those of Kapβ2-substrate complexes. Both unliganded and substrate-bound karyopherins are more elongated along their superhelical axes compared to the Ran-bound molecule, consistent with previous SAXS studies of Kapβ2 (Fukuhara et al., 2004).
The four unliganded Kapβ2s in the asymmetric unit show obvious conformational heterogeneity. We used three independent methods to analyze conformational heterogeneity. The first involves several different pairwise superpositions (CCP4, 1994): (1) we scanned the length of Kapβ2 chains superimposing groups of 1–20 HEAT repeats, (2) we superimposed A helices of each repeat and examined B helices for changes within HEAT repeats, and (3) we superimposed B helices and examined A helices of the next repeat for changes between HEAT repeats. The second method of analysis involves clustering rotation vectors using the program DynDom (Hayward and Berendsen, 1998). The third method involves analysis of the spatial distribution of individual atomic B factors in a single protein chain using the program TLSMD (translation/libration/screw motion determination; Painter and Merritt, 2006). The first two methods compare pairs of structures, whereas the third analyzes single protein chains.
When the structure was refined without using any NCS constraints (Table S1, refinement 1), unliganded Kapβ2 chains A and D are similar (Cα root-mean-square deviation [rmsd] 1.2Å) and chains B and C are also similar (Cα rmsd 1.0Å). In contrast, other pairwise comparisons give a Cα rmsd of 2.6—3.2Å. Given the similarity of the AD and BC pairs, the two pairs were constrained by NCS in the final structure reported in Table 1. Structural comparisons below are therefore limited to chains A and C.
Unliganded Kapβ2s are similar in their central regions. Exclusion of terminal repeats H1–H4 and H19-H20 decreased Cα rmsd from 2.9Å for all residues to 1.8Å (Table 2). Moreover, when superimposed at H8–H13 (Cα rmsd < 1.0Å), their termini deviate up to 11 Å (Figure 2A). HEAT repeats at the N and C termini also have higher B factors despite ~2200 Å2 of surface area buried by crystal contacts in these regions. For example, average B factors for H1–H4, H5–H18, and H19-H20 in chain B are 112, 65, and 84 Å2, respectively (electron density maps for the N and C termini are shown in Figures S1A and S1B).
Repeats H5–H13 of all four unliganded Kapβ2s are virtually identical, but their superhelical paths deviate at H14 (Figure 2A). To characterize conformational flexibility in the C-terminal arch, we analyzed each unliganded chain using the program TLSMD (Painter and Merritt, 2006). The molecule is partitioned through analysis of the spatial distribution of individual atomic B factors into 1–20 contiguous pseudo-rigid segments termed TLS groups. We used these multigroup TLS models as a starting point for analysis of large-scale motion in unliganded Kapβ2. The junction for the two-group TLS model mapped to residues 560–610 (H13-H14; Figure 2B). As partitioning progressed, this H13-H14 junction persisted, and additional TLS groups include the two flexible N and C termini segments (H1–H4 and H18–H20) discussed above.
Analyses of full-length unliganded Kapβ2 molecules using DynDom (Hayward and Berendsen, 1998) identified a hinge in H18, which is consistent with the flexible C-terminal segment (H19-H20). Removal of terminal H1–H4 and H19-H20 segments revealed a second hinge at H13-H14 which corresponds to the prominent TLS junction discussed above. A 9° rotation of rigid H5–H13 and H14–H18 segments relative to each other about this hinge axis explains the small conformational difference in C-terminal arches of chains A and C (Table 2; Cα rmsd H5–H13 is 1.3Å, H14–H18 is 0.5Å, and H5–H18 is 1.8Å).
Based on structural superpositions and two independent methods of identifying pseudo-rigid bodies in a molecule, the intrinsically flexible unliganded Kapβ2 can be divided into four moving segments. Two small segments, H1–H4 and H19-H20, are found at the termini. Two larger central segments, H5–H13 and H14–H18, are somewhat rigid elements that rotate relative to each other about a flexible hinge. Interestingly, results of structure refinements using NCS restraints are also consistent with the division of unliganded Kapβ2 into the four segments (Tables S1 and S2).
Kapβ2 binds RNA binding proteins through recognition of PY-NLS, a 20–30 residue long signal in the substrates (Lee et al., 2006). PY-NLSs are sequentially diverse, but share three common characteristics: structural disorder, overall basic character, and a set of a weakly conserved hydrophobic or basic N-terminal motif and a C-terminal R/K/Hx2–5PY motif. Crystal structures of Kapβ2 bound to two diverse PY-NLSs from splicing factors hnRNP A1 and M (Cansizoglu et al., 2007; Lee et al., 2006) show that the two NLSs bind a common interface (B helices of H8–H18) on the structurally invariant Kapβ2 C-terminal arch (H2–H20 Cα rmsd is 0.8 Å). Because the karyopherins in both substrate complexes are virtually identical, we use the hnRNP A1-NLS complex to represent the substrate-bound state, and chain A in the unliganded Kapβ2 crystal represents the unliganded state.
Although overall structures of unliganded and substrate-bound Kapβ2s appear similar, a 4.2 Å Cα rmsd suggests substantial conformational differences (Table 2). With the exception of the first four HEAT repeats, most of their N-terminal arches are similar (H5–H13 Cα rmsd is 1.3 Å). Thus, conformational differences mostly map to the C-terminal arch. Like unliganded Kapβ2, analysis using DynDom (Hayward and Berendsen, 1998) to compare unliganded and substrate states also identified a flexible hinge at H13-H14. Subdomains on both sides of the hinge, H5–H13 and H14–H20, behave like rigid segments (Cα rmsd for H5–H13, H14–H20, and H5–H20 are 1.3, 0.9, and 4.1 Å, respectively; Table 2), and NLS binding rotates one segment relative to the other ~16° about the hinge axis (Figure 3). This movement originates from the cumulative effect of small rotations between helices H12B, H13A, H13B, H14A, and H14B within the flexible H13-H14 hinge.
Interestingly, even though RanGTP binding results in large conformational changes in the Kapβ2 N-terminal arch, the C-terminal arch in Ran- and substrate-bound states are quite similar (H9–H18 Cα rmsd is 1.4 Å; Lee et al., 2006). Furthermore, comparison of H8–H20 (N-terminal arches removed as a result of large conformational changes) in the unliganded and Ran states again identified a flexible hinge in H13-H14 that rotates rigid segments H9–H13 and H14–H18 ~18° relative to each other (Cα rmsd for H9–H13, H14–H18, and H9–H18 are 1.5, 1.0, and 3.2 Å, respectively). The hinge axes that relate unliganded Kapβ2 to substrate- and to Ran-bound conformations are also somewhat similar (Figure 3). The C-terminal arches in Ran and substrate states are probably similar because in the former, Kapβ2's long H8 acidic loop occupies that arch and much of the loop overlaps spatially with bound NLSs (Cansizoglu et al., 2007; Chook and Blobel, 1999; Lee et al., 2006). Thus, conformational similarity in the C-terminal arch in the two ligand states supports the idea that the H8 loop is a pseudo-NLS (Lee et al., 2006).
The same rigid segments, H9–H13 and H14–H18, rotate relative to each other in the different unliganded Kapβ2 chains and between unliganded, substrate-, and Ran-bound Kapβ2s. Intrinsic segmental flexibility observed in the unliganded chains suggests varying degrees of rotation about the hinge. Paradoxically, similarity of the C-terminal arches in the NLS- and pseudo-NLS/Ran-bound Kapβ2s suggests a discrete energetically favorable arch conformation that binds ligands. What is the range of motion about this hinge? Does a range of rotation about the hinge allow fine-tuning of the substrate binding site to accommodate diverse PY-NLSs that vary significantly in length and composition? Or accommodate entirely new classes of undiscovered NLSs? Or accommodate interactions with nucleoporins? Crystal structures of Kapβ2 in complex with longer and more diverse PY-NLSs such as those in HuR and TAP (Fan and Steitz, 1998; Lee et al., 2006; Truant et al., 1999) and structures of Kapβ2-nucleoporin complexes will shed light on these questions.
Ran binds in the N-terminal arch of Kapβ2, contacting B helices of H1–H4, H7-H8, and the H8 acidic loop (Chook and Blobel, 1999). Dimensions of the Kapβ2 N-terminal arch change upon Ran binding (Figure 4C). The arch opens as the width of its base increases by 13 Å compared to unliganded and substrate-bound structures, enabling Ran to fit between the interfaces with H1–H4 and the H8 loop (Figures 4A and 4B). The C-terminal third of the N-terminal arch (H9–H13) does not contact Ran, and is similar between unliganded and Ran states (Cα rmsd 1.5 Å; Figure 4C). However, large changes in helical orientations occur within and between each HEAT repeat from H1 to H8. These large helical reorientations occur as helical content of the A, B, and connector helices changes, particular at helix termini, and connector loops also change in conformation. HEAT repeats move both laterally toward the dome of the arch and along the superhelix toward the N terminus to form a larger N-terminal arch.
A second obvious conformational difference between unliganded and Ran-bound Kapβ2s is found at the H8 loop. Proteolysis studies have suggested that the loop is exposed in unliganded and substrate-bound Kapβ2s (Chook et al., 2002). This was confirmed by both the Kapβ2-substrate structures (Cansizoglu et al., 2007; Lee et al., 2006) and is now also confirmed in the unliganded Kapβ2 structure. The H8 loop in the unliganded Kapβ2 crystals is biochemically intact, but only 13 of its 62 residues are observed, indicating that most of the loop is indeed mobile and disordered. Electron density is present only for residues 312–319 and 370–375 (Figure 5). Ordered loop residues 312–319 emerge from helix H8A and residues 370–374 precede helix H8B (Figure S2), and these have similar structures in unliganded and substrate-bound Kapβ2s. Residues 312–319 are in similar positions in all Kapβ2 structures, but residues 370–374 have shifted to direct the loop away from the arch in the unliganded and substrate-bound structures (Figure 1) (Lee et al., 2006). In contrast, in the Ran complex, H8 loop residues 332–340 and 363–373 form a platform that interacts with the basic patch of Ran while the rest of the loop resides in the C-terminal arch (Chook and Blobel, 1999). In summary, the concave surface of the unliganded C-terminal arch is free to bind substrate. The structure of unliganded Kapβ2 provides additional evidence that the H8 loop is flexible and does not reside in the C-terminal arch until Ran is present. Interactions with Ran changes the conformation of the loop, converting it into a pseudo-NLS to displace substrate from the C-terminal arch.
When Ran is absent, the N-terminal arches have almost identical dimensions (85 Å height and 58 Å width in its base) that are too small to accommodate Ran. Segments H5–H13 have similar conformations (Cα rmsd ~1.1 Å), but segments H1–H4 show conformational heterogeneity (Table 2; Figures 4A and 4B). H1–H4 is intrinsically flexible, with high B factors (>100 Å2), weak electron density, and a different conformation in every structure (Figure S3; Cansizoglu et al., 2007; Chook and Blobel, 1999; Lee et al., 2006). Structural differences here are of a continuous nature, with changes in helical orientations within and between HEAT repeats. Because the disordered H8 loop and the H1–H4 segment appear to be most flexible in the N-terminal arch, we speculate that either or both regions may serve as capture sites for initial interactions to "reel" in Ran. This is then followed by helical rearrangements in H5–H8 to optimally position the GTPase in the N-terminal arch and the rest of the H8 loop in the C-terminal arch. Kinetic studies of Ran binding and substrate dissociation will be necessary to investigate this model.
The stretched or expanded Ran-bound N-terminal arch is likely in a strained or higher-energy conformation. Transition to this high-energy conformation is balanced by large binding energy from Ran interactions, and subsequent removal of the GTPase relaxes it to a lower energy state. Karyopherin flexibility enables this allosteric phenomenon and was proposed for energy storage in Kap95 and Cse1 (Conti et al., 2006; Lee et al., 2005; Matsuura and Stewart, 2004). The ability of the N-terminal arch to change conformation along most of its length may also facilitate binding of additional ligands. Impβ binds substrate PTHrP in its N-terminal arch (Cingolani et al., 2002). Conformational flexibility of Kapβ2's N-terminal arch may also enable binding of undiscovered substrates in that region. Finally, N-terminal arch flexibility is also likely to be important for nucleoporin interactions.
In summary, conformational heterogeneity in Kapβ2 can be organized into three major segments. The N-terminal H1–H8 segment shows large changes along its entire length upon Ran binding. Segments H9–H13 and H14–H18 in the C-terminal arch are rigid bodies that rotate about a flexible hinge to bind NLSs and the H8 loop. Three additional small flexible segments are also observed. The flexible C-terminal H19-H20 segment is detected in most structural comparisons (Figure S4). The N-terminal H1–H8 segment can be further divided: intrinsically flexible H1–H4 is different in every structure, whereas H5–H8 changes conformation only when bound to Ran.
Impβ and its Saccharomyces cerevisiae homolog Kap95p are the best-studied Kapβs. Many structures of Impβ have been determined, but most are of the N-terminal half of the molecule (Bayliss et al., 2000, 2002; Cingolani et al., 2002; Lee et al., 2000; Vetter et al., 1999), and thus unsuitable for analysis of conformational flexibility along the superhelix. Three crystal structures of full-length Impβ are available: two crystal forms of Impβ bound to the IBB domain of Kapα and a SREBP2 complex (Cingolani et al., 1999; Lee et al., 2003). Two crystal structures are also available for full-length Kap95p, which is 33% identical to Impβ. These are Kap95p bound to RanGTP and Kap95p bound to a Nup1p fragment (Lee et al., 2005; Liu and Stewart, 2005). We performed domain motion and TLS group analyses on both pairs of structures to examine potential segmental architecture analogous to those in Kapβ2.
Hinges in Impβ were previously reported to rotate H1–H11 by 10° with respect to H12–H17, and to rotate the latter by 10° with respect to H18-H19 in different Impβ-IBB crystal forms (Cingolani et al., 1999). Hinge analysis of the IBB and SREBP complexes located a flexible hinge in H13 (Figure 6A). The N-terminal H1–H13 segment (Cα rmsd is 1.9 Å) swings 22° about the hinge axis with respect to the C-terminal H14–H19 segment (Cα rmsd is 1.1 Å), changing the superhelical pitch by 18 Å to bind conformationally diverse substrates. The N-terminal segment appears not to be rigid, with small changes in helical orientations along its length.
Similar analyses of the Kap95p structures located a flexible hinge at H14-H15. In this case, subdomains on either side of the hinge are H6–H14 (Cα rmsd is 1.5 Å) and H15–H19 (Cα rmsd is 1.2 Å). These segments rotate 38° relative to each other, resulting in a superhelical pitch difference of 10 Å. TLS group analysis maps a junction between TLS groups to H14 (residues 598–600) consistent with the position of the flexible hinge. Finally, examination of the Kap95p N-terminal arch alone (H1–H14) identified another hinge at H5, where segment H1–H4 (Cα rmsd is 0.7 Å) rotates 11° relative to segment H6–H14 (Cα rmsd is 1.5 Å).
In summary, like Kapβ2, conformational heterogeneity in Impβ and Kap95p is also segmental. Impβ can be divided into three segments that rotate about two flexible hinges in H12-H13 and H18 (Cingolani et al., 1999), and Kap95p can also be divided into three segments, with flexible hinges at H5 and H14-H15. Structures of full-length unliganded Impβ and Kap95p are not available, but a SAXS model of unliganded Impβ shows this state to be significantly more extended than the Ran or substrate states (Fukuhara et al., 2004). Crystal structures of full-length unliganded Impβ/Kap95 and additional structures of full-length ligand-bound proteins will be necessary for more comprehensive analysis of conformational heterogeneity in this nuclear import pathway.
The crystal structure of unliganded Kapβ2 has an overall superhelical structure similar to those of Ran, hnRNP A1-NLS, and hnRNP M-NLS complexes, confirming previous SAXS studies that the different Kapβ2 states adopt extended S-shaped structures. More importantly, the four Kapβ2 chains in the asymmetric unit show conformational heterogeneity, allowing characterization of intrinsic flexibility. Comparison with substrate- and Ran-bound Kapβ2s also showed significant conformational differences. Conformational flexibility analysis using three independent methods of structural superpositions, clustering of rotation vectors, and B factor analysis revealed the moving parts. Kapβ2 can be divided into three major segments. Rigid segments H9–H13 and H14–H18 in the C-terminal substrate-binding arch rotate relative to each other about a flexible hinge in H13-H14. H1–H8 in the N-terminal arch shows continuous changes along its length upon Ran binding. Using the same approaches, Impβ and its yeast homolog Kap95p can also be divided into three major segments that rotate about flexible hinges, suggesting that conformational heterogeneity in import-Kapβs may be generally segmental.
Full-length human Kapβ2 (residues 1–890; GenBank accession number: AAB58254) was in a pGEX-Tev vector (modified from pGEX-4T-3) expressed as a GST fusion protein and was purified as previously described (Lee et al., 2006). Selenomethionine Kapβ2 was expressed in BL21 (DE3) cells using M9 minimal media as previously described (Lee et al., 2006), and its purification is similar to that for native Kapβ2. The purified native and selenomethionine proteins were concentrated to 20 mg/ml and 35 mg/ml, respectively, for crystallization.
Native Kapβ2 was crystallized by vapor diffusion in hanging drops using 3.2 M potassium formate, 0.1 M HEPES (pH 7.5), and 10% glycerol in the reservoir solution. The crystals were flash-frozen in liquid propane. Data (3.0 Å) from these crystals (space group P21, a = 129.9, b = 169.3, c = 141.1, and β = 93.1° with four molecules in the asymmetric unit) were collected at X-ray wavelength 0.97933 Å at 100K at beamline 19ID of the Advanced Photon Source, Argonne National Laboratory. Data were processed using HKL2000 (Otwinowski and Minor, 1997). MR using Kapβ2 fragments from the Kapβ2-Ran structure (Protein Data Bank [PDB] ID code: 1QBK) as search models failed, indicating significant conformational differences in both N- and C-terminal arches between the two states. The Kapβ2-Ran structure was the only Kapβ2 structure available at the time of structure determination.
Selenomethionine Kapβ2 was crystallized by vapor diffusion in hanging drops using 2.7 M potassium formate and 20% glycerol in the reservoir solution. Unfortunately, these crystals (space group P21, a = c = 108.5, b = 294.0, and β = 92° with four molecules in the asymmetric unit) were pseudo-merohedrally twinned. In an effort to obtain single selenomethionine Kapβ2 crystals, mixtures of selenomethionine and native proteins were crystallized. A 1:1 molar mixture of the two proteins gave single crystals (space group P21, a = 108.5, b = 294.0, c = 108.3, and β = 92.1°), and a 3.6 Å SAD data set (λpeak = 0.97903 Å) was collected at beamline 19ID, Argonne National Laboratory.
We performed MR using the program Phaser (McCoy et al., 2005) and multiple search models, each comprising a different Kapβ2 segment from the Kapβ2-Ran structure. Only a search model of Kapβ2 residues 396–864 (H9–H19 or C-terminal arch) resulted in a successful molecular replacement solution. This result suggests that the C-terminal arch of selenomethionine unliganded Kapβ2 is more similar to that of the Ran complex than the native unliganded Kapβ2, but the N-terminal arches of both unliganded Kapβ2s are significantly different from the Ran state. However, to avoid model bias, 72 selenium sites (of the total 104) were extracted from the partial MR model and iterative refinement of these heavy-atom sites allowed the heavy-atom model to be extended to 100 selenium sites. The last four seleniums belonging to N-terminal methionines were not located. Phase refinement followed by density modification with solvent flipping, both using the program CNS (Brunger et al., 1998), resulted in an electron density map where three of the four Kapβ2s in the asymmetric unit could be modeled using O (Jones et al., 1991) and Coot (Emsley and Cowtan, 2004). Density for the fourth and untraceable chain was weak, with density absent for many side chains. This incomplete and low-resolution model was not refined.
Instead, the unrefined selenomethionine Kapβ2 model was used as an MR search model for the 3.0 Å resolution native Kapβ2 data. Using the program Phaser (McCoy et al., 2005), the Kapβ2 segment corresponding to residues 88–656 (H3–H14 or N-terminal arch) gave a successful molecular replacement solution for all four molecules in the asymmetric unit, suggesting similarities in the N- but not the C-terminal arches between the selenomethionine and native Kapβ2s. Overall superhelical structures of the native unliganded Kapβ2 (refined), the selenomethionine unliganded Kapβ2 (unrefined), and the Kapβ2-Ran complex are compared in Figure S5. The resulting electron density map for the native Kapβ2 crystal was interpretable for all four molecules in the asymmetric unit.
MR was not successful with native unliganded Kapβ2 using Kapβ2-Ran as a search model because the two states show large conformational differences in both the N- and C-terminal arches (Figure 4C; Figure S5). However, we found an MR solution for selenomethionine Kapβ2 that corresponds to the C-terminal arch of Kapβ2 in the Ran state. Figure S5 shows conformational differences between selenomethionine, native unliganded, and Ran-bound Kapβ2s. The C-terminal arch of selenomethionine Kapβ2 is somewhat intermediate in conformation between native unliganded and Ran-bound Kapβ2s, but is similar enough to the latter for MR. This partial MR model allowed us to locate most of the 104 selenium sites without determining them de novo using SHELX (Sheldrick et al., 1993). Unfortunately, structure determination of the selenomethionine protein was hampered by low resolution, pseudo-translation, and weak SAD phases (phasing: Rcullis 0.76, phasing power 1.06, Se site B factors 41–85 Å2 when occupancies were set to 1). Fortunately, the N-terminal arch of selenomethio-nine Kapβ2 was similar to the native protein (Figure S5), and we could use this segment of the unrefined selenomethionine model to bootstrap our way to an MR solution for the higher-resolution native data set. Interestingly, when comparing the selenomethionine and native Kapβ2s, a hinge was also located at H13-H14 that rotates rigid segments H9–H13 and H14–H18 relative to each other.
There are four unliganded Kapβ2 chains in the asymmetric unit and the protein has an unusual nonglobular helical repeat architecture. Therefore, NCS restraints may be used in many different ways when refining the structure. Refinement of the model (residues 6–890 for Kapβ2 chains A, C, and D and residues 31–890 for chain B) was done with 50–3.0 Å data, using the program REFMAC5 (Murshudov et al., 1997) from the CCP4 package (CCP4, 1994). Results of 16 different refinement protocols are listed in Tables S1 and S2. Without NCS restraints (Table S1, refinement 1), Rfree is 28.4% and R factor is 24.6%. However, without the use of NCS, refinement is not efficient, as the ratio of observables to unknowns is low at 3.0 Å resolution and with a large asymmetric unit. Tight NCS restraints of all four chains resulted in very high R factors (Rfree = 42.6% and R factor = 40.5%; Table S2, refinement 7), suggesting significant conformational heterogeneity among the four chains and that NCS restraints should be relaxed.
Analysis of the structure that was refined without NCS restraints (Table S1, refinement 1) suggested that chain A is very similar to D, and chain B is very similar to C. When entire chains AD and BC are refined with tight NCS restraints, Rfree dropped by ~11% (Table S1). However, R factors are still significantly higher than without NCS restraints, suggesting that the chains may need to be divided into multiple NCS groups. Structural comparison of chains A and C from refinement 1 suggested that Kapβ2 could be divided according to conformational heterogeneity into four segments: H1–H4, H5–H13, H14–H18, and H19-H20. Segment junctions H13 and H18 show clear conformational differences. This information guided our division of the molecule into NCS groups. We experimented with dividing each chain into four NCS groups (H1–H4, H5–H12, H14–H17, and H19-H20) and using tight NCS restraints in REFMAC5 (Murshudov et al., 1997) to constrain respective groups within the AD and BC pairs (refinement 3). The 3% drop in Rfree indicated that relaxation of NCS constraints is warranted and that there is conformational heterogeneity of segmental nature even within the two similar pairs of Kapβ2. When contiguous segments are combined to generate trisegmented Kapβ2 (refinements 4–6), R factors increased except when the last two segments are combined. We chose that structure from refinement 6 (Rfree = 28.6% and R factor = 26.1%; Table 1) for analysis. Comparison of this structure with refinement 1 shows that they are virtually identical, with Cα rmsd 0.3 Å.
We also experimented with NCS restraints on all four chains (Table S2). Dividing Kapβ2 into four segments (H1–H4, H5–H12, H14–H17, and H19-H20) for refinement of all groups with tight NCS restraints decreased Rfree by 10%, again consistent with segmental conformational heterogeneity within the four chains. Relaxing NCS restraints of the H5–H12 and H19-H20 groups decreased Rfree further by about 4%, suggesting conformational differences within these among the four unliganded Kapβ2 chains. Interestingly, superposition of H5–H13 of chains A and C gives Cα rmsd of 1.3 Å, suggesting that differences may occur in side chains or in the H8 loop (always omitted when superimposing structures) rather than in helical arrangements. Superposition of H19-H20 of chains A and C (Cα rmsd 2.2 Å) suggests that this C-terminal segment is indeed conformationally heterogeneous in all four chains. Comparison of structures from refinements 1 and 16 shows that they are virtually identical, with Cα rmsd 0.2 Å. We also experimented with dividing Kapβ2 into NCS groups other than H1–H4, H5–H12, H14–H17, and H19-H20. These include four 5-HEAT repeat groups and combining two of our contiguous segments at a time to make three groups. In all cases, Rfree was to ~36% (data not shown).
TLS refinement (Painter and Merritt, 2006) with 12 TLS groups (three groups for each molecule) resulted in Rfree = 27.0% and R factor = 23.3%. However, because there was no improvement in the electron density map and the structure is similar to that in refinement 6, we did not deposit this TLS-refined structure in the Protein Data Bank. Finally, the weakest density occurs at the N-terminal region of Kapβ2 molecules, which is also part of the MR search model. To reduce model bias, we calculated simulated annealing omit maps (Brunger et al., 1998) to omit each of the first four HEAT repeats of Kapβ2.
We used three independent/orthogonal methods to analyze flexibility. The first method involved pairwise superpositions of HEAT repeats. We performed comprehensive superpositions: (1) scanned the length of Kapβ2 chains for groups of 1–19 HEAT repeats, (2) superimposed A helices of individual repeats and examined the B helices for reorientations within each HEAT repeat, and (3) superimposed B helices and examined A helices for changes between HEAT repeats. The Superpose program from the CCP4 package (CCP4, 1994) was used to determine Cα-Cα distance rms deviations. The Helix Packing Pair program was used to determine angles between helices (Dalton et al., 2003).
The second method to analyze conformational flexibility involved domain motion analysis by clustering rotation vectors (that relates two protein chains) using the program DynDom (Hayward and Berendsen, 1998). Domain motion analysis to locate hinged motions was performed using coordinates for unliganded Kapβ2 (chains A–D) and Kapβ2s in the Ran, hnRNP A1-NLS (chains A and B; PDB ID code: 2H4M), and hnRNP M-NLS (chains A and B; PDB ID code: 2OT8). The nine Kapβ2 molecules extracted from four coordinate files were subject to pairwise analysis for the presence of hinged conformational differences using the program DynDom (Hayward and Berendsen, 1998). The window length for these analyses was set at five residues and the minimum ratio for interdomain to intradomain displacement was set to 1.0.
The final method involved analysis of the spatial distribution of individual atomic B factors in a single protein chain using the program in TLSMD for TLS group analysis (Painter and Merritt, 2006). Individual structures with refined B factors were used. This procedure identifies the portions of the protein with similar atomic displacement/thermal factor characteristics and determines the optimal TLS segments that behave like a pseudo-rigid body. For each protein chain, this analysis partitions the molecule into TLS groups and further analyzes the pseudo-rigid body translational and rotational motion of each group and how well these parameters fit the refined atomic thermal parameters in the crystal structure.
Methods 1 and 2 locate conformational differences by comparing pairs of structures, whereas method 3 locates pseudo-rigid segments using a single protein chain. Finally, all figures were prepared using the program PyMOL (DeLano, 2002).
We thank Mischa Machius and Shae Padrick for critical discussions, and Diana Tomchick and Chad Brautigam for expert assistance with data collection. The U.S. Department of Energy, Offices of Science and Basic Energy Sciences (contract W-31-109-ENG-38) supported the Advanced Photon Source use. Y.M.C. is funded by National Institutes of Health grant R01-GM069909, Welch Foundation grant I-1532, and the University of Texas Southwestern Endowed Scholars Program.
Supplemental Data
Supplemental Data include five figures and two tables and can be found with this article online at http://www.structure.org/cgi/content/full/15/11/1431/DC1/.
Accession Numbers
Coordinates and structure factors of unliganded Kapβ2 have been deposited in the Protein Data Bank under ID code 2QMR.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Newcastle Upon Tyne
Tyne And Wear
07719 473 175
Newcastle Upon Tyne
Tyne And Wear
07719 473 175
Hello and welcome
You may be reading this if you're struggling with difficult feelings or experiences. At a time when you might be feeling lost and nothing seems clear, counselling offers you a safe space and time to feel really listened to, understood and accepted. Exploring your feelings, hopes, fears, relationships and choices, can support you in coping with the events and issues in your life and enable you to consider the options available to you.
I offer counselling that is tailored to you as an individual and which is built around a supportive and non-judgmental relationship, whatever your background or circumstances. My discreet and comfortable counselling service is available in Newcastle upon Tyne and in Hexham, Northumberland.
A fully qualified and experienced counsellor I am a registered member of the BACP and accredited by the UKCP. I have worked with clients to overcome a broad range of issues including depression, panic, anxiety, grief, trauma, addiction, relationship issues, self esteem and anger management issues. I offer solution-focused brief therapy, short-term counselling or longer-term depth therapy, according on your needs and wishes.
By combining tools from person centred counselling, body psychotherapy, Gestalt and Transactional Analysis as well as creative therapies I can help you to gain a deeper understanding of the issues you are facing so that you can find your way forward.
Counselling works best when the relationship between counsellor and client is strong, so it's important to find the right therapist for you. If you'd like to talk to me before deciding to arrange a session, please feel free to call or send me an email. Or you can contact me to arrange an initial session to talk about your issues and see if I might be the right counsellor for you.
I look forward to hearing from you. If you need to talk, I'm here to listen.
I gained my qualifications at the Northern Guild of Psychotherapy in Newcastle. The Guild has been training counsellors and psychotherapists for over 25 years and is renowned for its in-depth and rigorous training programmes. The qualifications I hold are accredited by the UKCP and the BACP.
Professional Registration:
I have been working successfully as a psychotherapeutic counsellor for six years in the NHS, within private practice in Newcastle and Northumberland and for Rape Crisis.
I've worked with a huge range of clients: both men and women, people ranging in age from 18 to 75, and with diverse backgrounds and life experiences.
I have worked with clients to overcome a broad range of issues including:
I provide solution-focused brief therapy, short-term counselling or longer-term depth therapy, according clients' needs and wishes.
Member organisations
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Sessions last for 50 minutes and are charged at £45, payable by cash, online bank transfer or via text service 'Paym' before/at the beginning of each session.
If you would prefer to pay for blocks of sessions, please let me know and we can discuss this.
I have comfortable and discreet consulting rooms in Newcastle and in Hexham, Northumberland.
Free and on-street parking is available near each location and my Newcastle practice is easily accessible by Metro.
When you book your first appointment I will provide you with the full address and a map with directions.
If you're interested in having some counselling but can't make it to my clinic or if you live further afield, work abroad, travel frequently or have a busy schedule, I can help you conveniently and effectively via online counselling. Please see my website for further information.
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text:
Today, winter in Spain
December 8th, 2012, Spain
Tags: aielo
Tags: aielo
Stick around, there is more:
My little muse
Is no longer a willing participant to my creative process.
I am not sure when exactly this twist of events happened, but it has been awhile. I miss his unassuming eyes … Continue reading →
How we snubbed Madrid, overheated and stumbled on gold in Zaragoza
You often ask how we decide where to go. Here is a perfect example.
Yesterday morning I had an apartment organized for Madrid. All was well – free parking, plenty of … Continue reading →
I found my Spain
Tapas are not just a tourism gimmick, they are a real, delicious part of Spanish life. In the big cities they are everywhere, especially in the evenings when they decorate … Continue reading →


text:
Server-based computing with the free X2Go terminal server
Light at the End of the Tunnel
Admins have more interesting add-ons, such as x2gospyglass, which is useful for teachers; it displays thumbnails of student desktops, organized by classroom, Posix group, or individually.
X2Go is one of the most substantial remote access open source software projects available. It impresses with its selection of clients, extremely flexible administration, comprehensive selection of tools, and state-of-the-art authentication methods.
X2Go has also convinced other projects and many schools; the report in the "Linux4Afrika and X2Go" box explains why the development aid project switched from Edubuntu to X2Go. Also, X2Go is interesting for anyone wanting to give multiple users access to a single system, if bandwidth is insufficient for VNC, or if you need file shares and audio forwarding on top.
R-zwo-R in Rohrdorf, Germany [16], offers service-level agreements for anyone interested in running X2Go in an enterprise environment: A major part of the revenue generated from support goes directly to X2Go. The developers deliberately avoided a dual license.
For comparison's sake, Sun charges EUR$ 90 per user and more than EUR$ 200 for each hardware-based Sun Ray client.
An ISO image of the Live CD is now available [17].
- X2Go: http://x2go.berlios.de/index-en.html
- Sun virtual desktop infrastructure: http://www.sun.com/software/vdi/index.jsp
- "Das Sun-Ray-Konzept" by Jens-Christoph Brendel, Linux Technical Review 06: Server Based Computing, pg. 62 (German)
- Sun Ray server 4: http://www.sun.com/software/sunray/index.jsp
- Debootstrap: http://www.debian-administration.org/articles/426
- NoMachine: http://www.nomachine.com
- FreeNX: http://freenx.berlios.de
- FUSE: http://fuse.sourceforge.net
- SSHFS: http://fuse.sourceforge.net/sshfs.html
- Linux4Afrika: http://www.linux4afrika.de/vision.html
- Maemo: http://maemo.org
- OpenPGP Card: http://www.g10code.com/p-card.html
- Installation guide by Linux4Afrika: http://www.linux4afrika.de/x2go
- "Terminaldienste mit X2Go" by Heinz-M. Graesing, Linux Technical Review 06: Server Based Computing, pg. 20 (German)
- Luma: http://luma.sourceforge.net
- R-zwo-R: http://www.rzwor.de (German)
- Live CD: http://developer.berlios.de/project/showfiles.php?group_id=8454
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Biggest Kernel Release Ever
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For Faculty
The Writing Center is committed to collaborating with faculty from all disciplines to assist students with their writing assignments and promote retention and success.
Here are some steps that faculty can take to insure that their students have a high quality tutoring experience:
- Familiarize yourself with the Writing Center's policies and procedures as listed on the main Writing Center page and under the FAQ's Related Link.
- Include our website on your syllabus and encourage students to consult our website for information on hours and locations, tutor schedules, a summary of what to expect and how to prepare, links to additional resources, and answers to FAQ's
- Provide students with written instructions for assignments; clearly detail expectations for content, organization, grammar, style, and documentation. Remind students to bring their assignment sheet when they come for help
- Encourage students to come to the Writing Center long enough before the paper is due so as to be able to make the suggested revisions
- Remind students that the Writing Center can help with general skill development
- Incentivize responsible use of the Writing Center
- Consider making a Writing Center tutoring session a condition of being allowed to rewrite a paper for a higher grade
- If you give extra credit, consider making it contingent on students coming at least a full day before the assignment is due and coming prepared (with their assignment sheet)
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Fact Sheets are routinely provided to all faculty teaching composition courses. If you wish to distribute copies to students in other classes, we will gladly provide them. Click here to request fact sheets. Be sure to include your mail stop and the number of fact sheets you would like.
The Writing Center is happy to send a tutor to your
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Tuesday, January 29, 2013
Next Door Inc. is on the verge of a major capital goal.
The nonprofit agency needs $1,860 to match a $100,000 grant made last year by Luhr Jensen Company.
The Next Door has until Jan. 31 to raise the remaining funds, according to Heidi Venture, NDI development manager.
This is the first matching grant ever received by NDI, and "we really weren't sure what would happen," Venture said. "Our supporters came through for us, and we even had donations from 45 people who have never given to us before."
In 2009, Phil and Judy Jensen loaned NDI $300,000 to help pay for the renovation of the agency's new building in Hood River while NDI waited for grants to be funded.
"When our loan was due in June 2012, they told us they'd be happy to give us $100,000 as a matching fund in honor of Phil's dad, Luhr Jensen, with a final matching date of Jan. 31, 2013," Venture said.
"Phil and Judy are great people, and their offer couldn't have come at a better time," said Janet Hamada, NDI director.
"In discussing what they wanted the Luhr Jensen Match Fund to accomplish, the Jensens did not have a particular focus; instead they let us decide if we wanted to earmark it to one of our 28 programs or for administration in general," Hamada said.
"We decided that we would use the match for any individual donations, including those that are program-specific.
"For example, donations to Klahre House School, New Parent Services and Big Brothers-Big Sisters have all been matched. This has been a help to the programs. General donations have also been matched, which has helped all our programs."
Toward the match, NDI received 14 donations in amounts over $1,000, all from individuals — except one from the congregation of the Hood River Alliance Church.
The largest matchable donation was $10,000; the smallest, $10.
"So far, we've been able to match 256 donations for a total of $98,140. We are very grateful to the amazingly generous people of the Columbia Gorge," Venture said.
Anyone who wants to donate to the Jensen matching fund can call Venture at 541-436-0303, or visit www.nextdoorinc.org and click the Donate button.
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Parkdale third graders sing "12 Disaster Days of Christmas"
Welcome to your sing-able Christmas gift list. What follows is an emergency rendition of "12 Days of Christmas" – for outfitting your home or car in case of snow storm, earthquake, flood or other emergency. Read it as a simple list, or sing it to the tune of "12 Days" – you know, as in " … and a partridge in a pear tree…" Not to make light of it, but the song is a familiar framework for a set of gift ideas that you could consider gathering together, even if the recipient already owns items such as a bunch of coats, tire chains and flashlights. Stores throughout the Gorge are stocked up on all these items. Buying all 12 days might be prohibitive, but here are three ideas for checking any of the dozen off your list (notations follow, 1-12.) The gift items needed to stay warm, dry and safe are also coded to suggest items in your abode (A) in your car (C) or both (B). 12 Gallons of Water (A) 11 Family meals (B) 10 Cans of propane (A) 9 Hygiene bags (B) 8 Packs of batteries (A) 7 Spare coats (B) 6 Bright red flares (C) 5 Cozy blankets (B) 4 Tire chains (C) 3 Flashlights (B) 2 cell phone chargers (B) 1 And a crush-proof first aid kit (B) Price ranges? Here's a few quotes for days Three, Two, Four and Nine: n A family gift of flashlights (three will run $15-30, Hood River Supply, Tum-A-Lum) n Cell phone chargers (two will run $30-60) n Tire chains (basic set, $30, Les Schwab, returnable if unused for the winter) n Family meals ($100 or so should cover the basics for three or four reasonably well-fed days) n The home kit should be kept in a handy place near an exit, and remember that water needs to be replenished every few months. If you have a solid first aid kit already, switch out the gift idea with "and-a-sto-o-u-t- tub-for it-all …" Otherwise, it's a case of assembling your home or car kits and making sure all members of the family know what the resources are and how to use them (ie flares and propane). Emergency situations are at worst life-threatening, at best deeply uncomfortable if you and your family are left without power for an extended period, or traveling and find yourself in a situation where you need to wait out a storm, lengthy traffic delay, or other crisis. Notes on the 12 gift ideas: 12 – Gallons of water: that's one per person in a four-member family to last for three days, the recommended minimum to be prepared for utility outages. 11 – Easy-open packaged goods, energy bars, dried food and nuts are good things to include for nutrition. Think of what your family of four needs for three days to stay fortified and hydrated (see number 12). Can-opener also recommended 10 – If you have a propane camping stove, keep extra fuel handy. 9 – Hygiene bags: put packaged moistened towelettes, toilet paper, and plastic ties in large garbage bags (for personal sanitation) Resource list courtesy of Hood River County Emergency Management, Barbara Ayers, manager/ 541-386-1213. The county also reminds residents to Get a Kit, Make A Plan to connect your family if separated, and Stay Informed. See www.co.hood-river.or.us to opt-in for citizen alerts. Enlarge


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John Hastings
I made a modest living in photography shooting kid's sporting events, beauty pageant candids, and weddings. Unfortunately, those events ate up my weekends and 10 years ago I decided to just shoot for fun, friends, and the occasional paid gig to buy some new piece of equipment.
Now the kids are grown up and I'm coming back to my love of photography.
Find me at: http://flickr.com
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Q: Why are some of the contest photos obviously photoshopped?
A: Do you know?


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Pulvex Ltd
diamond blades, Diamond Grinding Wheels, Asphalt Blades
Pulvex is a family run business that designs, manufactures and sells high performance diamond blades, diamond core drills and diamond grinding wheels directly into the construction industry.
The latest technological advances, the highest grades of diamond and state of the art bonding systems are utilized to produce the best performing diamond blades available.
We have a fantastic range of diamond blades that cut hard clay, engineering bricks, concrete, sandstone, Indian sandstone, block paving, asphalt and any other construction material.
Pulvex is happy to offer advise on selecting the best diamond blade for your requirements and is frequently able to supply diamond blades that solve extremely difficult cutting problems.
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