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A handful of people got a chance to live out their James Bond fantasies thanks to a joint ad campaign from Coke Zero and the upcoming Bond flick, "Skyfall."
The stunt is pretty brilliant. Unsuspecting travelers at Antwerp Central station in Belgium, thirsty for some Coke, step up to a vending machine and are prompted with the following question: "Want the chance to win exclusive tickets to Skyfall?" If their answer is yes, they're given a challenge -- they have 70 seconds to race to platform six.
Of course, this is a stunt and the stuff viral videos are made of -- Coke Zero isn't going to make the task as easy as it sounds. There are plenty obstacles planted along the way, like spilled oranges (one guy totally eats it!), a dog walker with tangled leashes and frustrating joggers who won't get out of the way. It's amazing to watch. We second Digital Trends' plaintive plea: "Coke Zero, we beg of you: Bring this stunt to America."
Watch a handful of folks get their Bond on in the below video.
CORRECTION: A previous version of this article stated the stunt took place in the UK. It took place at Antwerp Central station in Belgium.
The Morning Email helps you start your workday with everything you need to know: breaking news, entertainment and a dash of fun. Learn more


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Willien Strutz
Phone: In South Africa ~ 012 998 5598
Outside South Africa ~ +27 12 998 5598
Cell ~ +27 82 444 6950
Email: firstname.lastname@example.com
God's Garden Gifts
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Once you have started to learn about these liquid miracles from God's garden, and their
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Last Updated: 03.01.2017
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Midem, the International B2B Music market
6-9 June 2017, Palais des Festivals, Cannes, France
Enjoy everything that Midem has to offer, including business-driven conferences, live shows and networking events.
Register now and save €245 on the walk-in rate
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Organise meetings in advance and meet the right people with MIDEM's online database.
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Russian Adoptee Sent Back to Moscow Because of Challenging Behavior
There is a 7-year-old boy named Artyom Savelyev who is making the headlines this weekend. He was adopted by a Tennessee woman interested in being his mother, adopting him from the boy's native Russia. But something went very wrong and his adoptive mother, Torry Hansen, sent the boy back to Russia. The manner in which she sent the boy back is causing outrage in the international community. It seems she put the 7-year-old boy on the plane unattended with note saying she no longer wanted to parent him due to his violent behaviors and severe psychological problems.
According to one CBS news report The Kremlin children's rights office shared the contents of the note which read:
"This child is mentally unstable. He is violent and has severe psychopathic issues. I was lied to and misled by the Russian Orphanage workers and director regarding his mental stability and other issues. ... After giving my best to this child, I am sorry to say that for the safety of my family, friends, and myself, I no longer wish to parent this child."
The child's adoptive grandmother reported that Artyom, who was renamed Justin, drew a picture of their house burning down and that the family feared for their safety. She also talked about incidents of hitting, kicking and spitting.
The reaction from Russian officials has been to threaten to halt all child adoptions by U.S. families. Russian President Dmitry Medvedev and Russian Foreign Minister Sergey Lavrov are reportedly outraged by the adoptive mother's actions which could lead to a permanent suspension of American adoptions of Russian children.
It is certain that there will be a lot of different viewpoints on this, but as the mother of a child with special needs and behavioral challenges, my predominant emotion upon reading this news is great sadness. I imagine a scenario where my son would be in this situation. Would someone send him packing? Or would they take the time to try to help him? There are just so many unanswered questions that may come to light as this case is investigated. Did the boy have any diagnoses? Was he abused? Did he have any developmental delays, mental illness, ADHD or even an autism spectrum disorder?
Did the mother try to reach out for help or support? What about teachers? Counselors? Mental health agencies? Did this family make any attempt to help this child before sending him away? What was the mother's mental state when she sent her adoptive son on a plane, alone, back to Moscow? And what things occurred to cause a family to be so terrified of a 7-year-old child?
Tonight I find myself thinking of this boy and the haunting image of his sad and hollow eyes. I wonder what will become of him or other children like him who are deemed as too challenging or difficult to handle. And what will become of the Russian orphans who now may have nobody to adopt them due to this incident?
It is a story for which there seems no happy ending.
What are your thoughts? Please share them here. We appreciate your perspective and insights.


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When you open a small independent store, in a lot of ways it's a form of personal expression.
We just like stocking the store with stuff we like, topics that interest us.
A lot of us tend to think that we're somewhat singular. Somewhat freakish.
Our tastes are all over the place. Who else is going to be into Jeffrey Brown, sideshow performers, Mark Ryden, The Believer, Anthony Bourdain, zines, both Haruki AND Takashi Murakami, serial killers, Tom Waits, exploitation films, knitting, bondage, hacking, outsider art, Buffy the Vampire Slayer, David AND Amy Sedaris, Ben Snakepit, Howard Zinn, AS WELL as the Dark Knight?
Who else? Our customers.
We are SO not alone.


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Overall: 15-13 .536 W-L% (134th of 295)
Conference: 5-5, 4th in NAC
Coach: Tom Brennan (15-13)
PS/G: 72.8 (195th of 295)
PA/G: 73.0 (100th of 295)
SRS: -7.55 (210th of 295)
SOS: -7.36 (254th of 295)
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Our reasoning for presenting offensive logos.
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Hotfrog NewZealand provides information regarding My Corporate Guide in Pakuranga Auckland. My Corporate Guide is located at PO Box 51 719 Pakuranga Auckland 2140 New Zealand, Pakuranga Auckland 51 719 and provides Business Coaches services. Contact them on 0800 687 933 or by visiting them on their website http://www.mycorporateguide.com/.
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Video watch online Devanshi 25th December 2016 latest new full Episode 70 of Colors Tv drama serial Devanshi complete show Episodes by colorsTv. watch Devanshi Episode 70 online.
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First Tutors: Music helps you to locate a tin whistle tutor in West Midlands and across the UK. First Tutors: Music profiles West Midlands tin whistle tutors and others offering popular instruments like piano, violin and guitar. Grade and diploma tin whistle lessons are all catered for across West Midlands as well as tin whistle lessons just for fun. Please use our service to search for tin whistle teachers near you and select which tin whistle teachers best suit your needs.
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ApartmentD-2 Apartment D-2on the ground floorof a residential buildingin Promajna. It consists of: hall, living roomwithkitchen, bathroom, utility room, bedroomanda spacious terrace. Total net floor areawithparking spaceis58,36 m²
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Edward and Judy Fritts, formerly of Harriman, will celebrate their 40th wedding anniversary on June 9.
A reception celebration will be from 2 to 4 p.m. June 9 in rooms B and C of Cumberland Mountain State Park Restaurant near Crossville. Light refreshments will be served.
All relatives and friends are invited. The couple requests no gifts or cards. The occasion will be casual dress.
Michael and Karen Parkison of Kingston announce the forthcoming marriage of their daughter, Rachel Leann, to Ryan Bentley.
He is the son of Mark and Kelly Bentley of Knoxville.
The ceremony will be at 4 p.m. June 9 in Laurel Bluff Baptist Church.
The bride-elect is the granddaughter of Lane and Sylvia Parkison of Kingston, and Mitchell and Peggy Grosch of Manchester.
The city of Harriman and the Princess Theatre Partnership received the Preservation Award during the East Tennessee Historical Society's annual Awards of Excellence presentation.
"We commend the city of Harriman for its vision and commitment to preserving the Princess Theatre," said Society director Cherel Henderson.
"The success of the project demonstrates the importance of history and sense of place in creating character for a town."
Congratulations to Dale Fuqua, who took classes at Roane State Community College to become a certified EKG technician. Phlebotomy for Healthcare Professional Certified and will be taking the National American Medical Technologist exam in June. Much success to you, Dale.
Congratulations to Talisa Hall and Haley Goins, who graduated from Harriman High School. Haley is the daughter of Michael Goins, and Talisa is the granddaughter of Deborah Hill.
By Thomas Greene, for Roane Newspapers
In a change-of-responsibility ceremony earlier this month at Knoxville's West High School auditorium, Command Sgt. Major Vincent S. Roach took the reins as the regimental command sergeant major of the National Guard's 278th Armored Cavalry Regiment.
Roach assumed the responsibility from Command Sgt. Maj. Daniel Jennings of Clarksville.
There is something in the Southern character that seeks good food and fellowship in roughly equal portions.
Harriman Lions Club will provide both this weekend during its fundraiser, The World's Greatest Pancake Breakfast.
The breakfast will be from 7 to 11:30 a.m. May 26 in Harriman Diner on Roane Street.
The McKameys, a premier East Tennessee family gospel-singing group from Clinton, will host their annual Hometown Singing on June 8-9 in Clinton Second Baptist Church.
The Inspirations of Bryson City, N.C., will be the special guest at 7:30 p.m. Friday.
Saturday's special guest will be the Primitive Quartet of Candler, N.C. at 6 p.m.
Reserved seating is $15; general admission is $13.
Call 800-254-3047 or 865-457-3678 for details.
Kingston First Baptist
"Amazing Wonders Aviation" is the theme of Kingston First Baptist Church's vacation Bible school. Classes for students who have completed kindergarten through sixth grade are from 9 a.m. to noon June 18-22 in the church gymnasium at 215 N. Kentucky St. Register online at www.kingstonfbc.org, or call 376-6041.
Kingston United Methodist/Bethel Presbyterian
25 Years Ago
Plans for a long-anticipated Food Lion warehouse and distribution center in the Roane County Industrial Park were dashed when the Food Lion corporate office decided to locate in Georgia instead. According to Food Lion officials, the decision was determined by cold, hard figures, and had nothing to do with any shortcomings of the county.
10 Years Ago
When the HonorAir flight landed in Knoxville the evening of April 18, the organization successfully completed 12 flights taking more than 1,300 East Tennessee World War II and Korean War veterans to Washington, D.C., to see the memorials built to honor their sacrifices.
Among the 130 veterans on this most recent flight were Roane County's Clyfton Chandler, Eddie Owings and Charles Wilson, accompanied by volunteer guardian John Owings.
Copyright © 2017
The Roane County News is your source for local news, sports, events, and information in Roane County and Kingston, Tennessee, and the surrounding area.


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On Second Thought is a one-hour, daily news talk show that airs at 9 a.m. ET weekdays.
Our unlikely British sleuths are back on Saturday nights starting at 7 PM.
The GHSA state championships for football are right around the corner. There are 16 teams left on the road to the Georgia Dome and by late Saturday night all eight champions for 2016 will be... MORE
GPB Passport is the new member benefit that provides you with extended access to an on-demand library of quality local and national public television programming, including tens of thousands of hours of your favorite programs.
Mon., January 30, 2012 8:00am
Man Recounts Kidnapping Terror
A Georgia man kidnapped by gunmen in Nigeria's oil-rich southern delta says he watched in horror as two men in the front seat of his SUV were shot seven times.
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Superhero Books
LBST 5 years +5 years +
Batman will be swinging his way back into the cinemas this year - but this time, he'll be in LEGO Minifigure form!
- £3.99
- Save £4.00
A spin-off from The LEGO Movie (where Will Arnett's take on the Dark Knight stole the show), this film promises to pay tribute to all eras of the Caped Crusader and his adventures. The trailer has given a hint of just how funny this film will be and we're sure the whole family will love it.
This Ultimate Sticker Collection book is packed with over 1,000 colourful and reusable stickers inspired by the film and features Batman, his friend Robin, his enemy The Joker and various vehicles including the LEGO Batmobile. It gives children the chance to explore and decorate Gotham City, brick by brick...
LBVG 5 years +5 years +
- £3.99
- Save £6.00
We cannot wait to see The LEGO Batman Movie and we're sure you and your family are just as excited. Featuring Batman (voiced by Arrested Development's Will Arnett) from The LEGO Movie, the film's trailer is absolutely hilarious and promises to give nods to all eras of one of DC Comics' finest superheroes.
This book goes behind the scenes of LEGO Gotham City and will help fans discover everything they want to know about this darkest of knights. From Robin to Batgirl, it covers all the heroes, villains, vehicles and locations from the film.
MVEY 8 years +8 years +
The Hulk features on the tactile cover of this Marvel book full of incredible trivia, facts and artwork.
- £5.99
- Save £12.00
Covering heroes, villains and those characters that flit in-between, it includes bizarre-but-true tales, quirky insights into characters including the Avengers, Spider-Man and Daredevil and so much more.
From why there are three different colours of Hulk (green, grey and red in case you were asking) to a look at the time that a frog gained Thor's powers, many of these revelations are astounding - and will delight anyone who loves Marvel, no matter what their age.
LGDC 7 years +7 years +
This 10-book collection gives young superhero fans the chance to learn more about Batman, Superman and many more of the heroes and villains from the DC Comics universe.
- £14.99
- Save £54.91
- Just £1.50 per book
Full of facts about the characters and their battles, it will tell you who their friends and foes are and focuses on some of the best builds you can make inspired by them.
Best of all, there's even an exclusive Minifigure of Batman included in the set!
BCEN 5 years +5 years +
Profiling more than 200 of Batman's friends and foes that walk the streets of Gotham City, this Character Encyclopedia is full of comic book art, facts and stats.
- £3.99
- Save £6.00
From his allies including Robin and Alfred Pennyworth to vicious villains like the Joker and the Penguin and even lesser-known characters including Robin's pet Bat-Cow, each character has their own page illustrated with up-to-date information and a stunning illustration from DC Comics.
A must for children and adults who are excited about seeing the Dark Knight face up to the Man of Steel in Batman v Superman: Dawn of Justice.
This four-book collection provides superhero fans with an introduction to the origins of Avengers Thor, Iron Man, Captain America and Hulk.
- £6.99
- Save £18.00
Sure to be enjoyed by Marvel fans of all ages, they are presented in a handy slipcase and the set even comes with 20 stickers to decorate the pages.
BVSM 7 years +7 years +
The first time the Dark Knight and Man of Steel have appeared on the big screen together, Batman v Superman: Dawn of Justice must rank as one of the year's most eagerly awaited films.
- £3.99
- Save £4.00
As the two heroes battle it out on the big screen, the 900 stickers in this activity-filled book will help fans crack secret codes, design new gadgets and save the world ? whichever hero's side they?re on.
Fire up the Bat-Signal and call in every superhero fan you know! This huge book from DK will tell you everything you could ever want to know about DC Comics' famous heroes and their evil enemies.
- £4.99
- Save £12.00
Packed full of facts, stats and the original comic book artwork, you will have the chance to read about Batman, Superman, Wonder Woman and The Joker.
With 12 character cards and an awesome poster also included, this book will keep DC Comics fans of any age enthralled and entertained.
Avengers fans... assemble!
- £4.99
- Save £12.00
Featuring all the favourite characters from the Marvel universe, this extensive book provides profiles of over 225 superheroes, their friends and their foes.
From Avengers including Captain America, Iron Man, Thor and the Incredible Hulk to villains like Red Skull, Loki and Thanos, you'll get to discover facts and stats about each character and their history and powers.
The book even comes with collector cards of the famous heroes and a pull-out poster!
MBSH 9 years +9 years +
This is a super story for superhero-mad children! An award-winning book written by David Solomons, it's a zesty and refreshing read full of funny situations.
- £3.99
- Save £9.00
Luke is a comic book-mad 11-year old who shares a tree house with his geeky older brother, Zack.
Luke's only mistake is to go for a wee right at the wrong time. While he's gone, an alien gives his undeserving, never-read-a-comic-in-his-life brother superpowers and then tells him to save the universe.
Luke is massively annoyed about this, but when Zack is kidnapped by his arch-nemesis, Luke and his friends have only five days to find him and save the world...
DCOM 5 years +5 years +
Superman, Batman and Wonder Woman all feature in the DC Comics Super Heroes Ultimate Sticker Collection!
- £3.99
- Save £4.00
Suitable for superhero fans aged 5 and over, this book will introduce them to all the characters, vehicles and weapons that have appeared in the comics, cartoons and films.
From great gadgets to just who makes up the Justice League, it's a super book - especially with the heroes set to arrive on the big screen in Batman v Superman: Dawn of Justice this year.
MYQ2 9 years +9 years +
This is a super book for superhero-mad children! An award-winning book written by David Solomons, it's a zesty and refreshing read full of funny situations.
- £4.99
- Save £2.00
Luke is a comic-mad 11-year old who shares a tree house with his geeky older brother, Zack.
Luke's only mistake is to go for a wee right at the wrong time. While he's gone, an alien gives his undeserving, never-read-a-comic-in-his-life brother superpowers and then tells him to save the universe.
Luke is massively annoyed about this, but when Zack is kidnapped by his arch-nemesis, Luke and his friends have only five days to find him and save the world...
MYBY 8 years +8 years +
For the past decade, many of Marvel's comic book heroes have come to the big screen in massively successful films, culminating in the Avengers coming together.
- £9.99
- Save £20.01
With the success of Ant-Man and the Avengers sequel, the brand shows no sign of slowing down and Marvel: Year by Year - a Visual Chronicle will reveal all you ever wanted to know about these superheroes and their colourful histories in the inspirational comic books.
Covering the likes of Spider-Man, the X-Men and Daredevil, the book features original artwork and classic covers, as well as information from leading Marvel historians. A brilliant collector's piece that spans back to Marvel's origins as Timely Comics in the 1930s, the book even has a foreword from the legendary Stan Lee.
With Book People's price, you'll be making a super saving too!
Find out all there is to know about the world's mightiest superheroes with this awesome collection from Marvel.
- £9.99
- Save £16.98
- Just £3.33 per book
With books based on Spider-Man, Iron Man and the Avengers, you can discover plenty of facts about these iconic characters' adventures, powers, friends and foes.
Throughout the books, you can learn all about how Tony Stark invented the Iron Man armour and find out about his glitzy life; discover how Peter Parker became Spider-Man and just who his enemies are; and read all about why the Avengers are the world's most powerful superhero team. With a giant poster also included, this is great for any superfan!
Over the past 12 months we have seen Batman and Superman facing up to each other and the Suicide Squad causing all sort of havoc... 2017 will see Wonder Woman and the Justice League appear on the big screen and if you want to find out more about these iconic DC Comics characters, this encyclopedia is essential.
- £9.99
- Save £20.01
Featuring original comic artwork, this book features over 1,200 characters including heroes, villains, friends and foes. There are features on the characters' most famous clashes and romantic encounters, while their famous vehicles and weaponry are also featured.
DC Comics has been entertaining the masses for over 75 years and this fully up-to-date book is lavishly illustrated and packed with facts any fan will love to pore over.
MTAE 10 years +10 years +
You've seen the films and read the comics, now read about over 250 Avengers characters and their adventures in this superhero-filled encyclopedia.
- £9.99
- Save £20.01
The most comprehensive volume of the team, it looks at how they evolved over the years and focuses on all the popular core members - Iron Man, Captain America, Thor, the Hulk. It also looks at their allies and foes, as well as their special powers.
20 of the most important plots are profiled and the comic book artwork is simply Marvel-lous. A must-have gift for all fans.
Coming with an exclusive Minifigure of Batman, this huge encyclopedia takes a look at LEGO's superheroes inspired by DC Comics - including the Dark Knight, Wonder Woman and Superman.
- £4.99
- Save £10.00
A comprehensive visual guide, it includes facts and figures about the heroes and their friends and foes. It even reveals all about their special weapons, cool gadgets and amazing vehicles.
This is a must for Master Builders who love superheroes.
What happens when the Justice League joins forces with the Bizarro League, a team of mixed-up, bizarre clone versions of themselves? When Bizarro's new home planet, Bizarro World, is threatened by Darkseid, he needs all the help he can get! Bizarro returns to earth and creates a new team of "heroes" like himself: the Bizarro League!
- £4.89
- Save £0.10
Help the caped crusader on his quests, dispatch Robin to fight crime and take down the dastardly Joker once and for all. The LEGO Batman Ultimate Sticker Collection takes you into the world of Gotham City and puts you in charge.
- £7.89
- Save £0.10
Capturing all the fun of LEGO Batman in an incredible, super-sized sticker collection, this super-sized LEGO Batman Ultimate Sticker Collection is packed with the colourful images and easy-peel stickers of all your favourite goodies, baddies and vehicles, including the mighty Batmobile! Featuring more than 1000 stickers from the LEGO Batman line of toys, simply read the captions in the book, turn the pages and choose the sticker again and again...
Meet The Avengers in an incredible, super-sized sticker collection. This bumper "Marvel Avengers Assemble! Ultimate Sticker Book" is packed with more than 250 easy-to-peel colour stickers. Join the Earth's mightiest heroes as they assemble to battle the most lethal villains the universe can throw at them. It features your favourite Avengers - Iron Man, Captain America, Thor, Hulk, Wasp and Ant-Man - and some of their most fearsome foes. Great scenes taken straight from the comics mean you can create your own action-packed adventures. Don't forget, you can use your stickers again and again. If you still want more, there are tons of cool facts about The Avengers to dazzle your friends.
- £4.49
- Save £0.50
From the bestselling author of Dirty Bertie. Stan Button had always dreamed of becoming a superhero. Then one day a letter arrives inviting him for an interview at the mysterious Mighty High School. To his amazement, Stan is offered a place - starting immediately. His superpower is 'non-conventional', as are those of his two new best friends, but lessons in supervillain-spotting and tight-wearing mean they are soon ready to defeat the evil Green Meanie. who might just be none other than Professor Von Hardbum, a teacher at their school. Each adventure will be heavily illustrated throughout with fantastic art by Nigel Baines, for truly brilliant comic-book style fun.
- £4.89
- Save £0.10
New guided reading series from DK designed to help kids learn how to read and learn to love reading. Marvel The Avengers Battle Against Ultron assembles your favourite Marvel Avengers into one action-packed book. Some Super Villians are just too powerful for one Super Hero alone - and this is where The Avengers come in! Learn all about Thor, Captain America, Iron Man, Black Widow and many more Marvel Avengers and how they have been using their powers to protect Earth for many years. Diaries, cartoons and posters help you explore the Avengers world, from their powers and abilities to the part they play in Earth's mightiest Super Hero team. Marvel The Avengers Battle Against Ultron is a "Reading Alone" level book, part of the new DK Reads series with three reading levels that help children become confident readers. DK Reads is created in consultation with literacy experts and adheres to leveled reading scheme guidelines so the grammar, vocabulary and content are spot on for each stage.
- £4.89
- Save £0.10
This is an all new activity collection with the ultimate blend of facts and fun. Perfect non-fiction books for kids, the Ultimate Factivity Collection is packed with exciting activities, fun challenges and interesting puzzles designed to inspire creativity. Bursting with puzzles, games, drawing activities and more than 500 stickers, alongside amazing facts and information, Marvel The Avengers Ultimate Factivity Collection keeps you entertained for hours. You can learn all about Iron Man, Captain America, the Hulk and many more Super Heroes, and use the stickers inside to create your own adventures. Packed with all your favourite Super Heroes and their enemies, you'll have so much fun with this exciting, interactive Factivity Collection. With Marvel The Avengers Ultimate Factivity Collection, learning has never been so fun! (copyright) 2014 MARVEL.
- £6.39
- Save £1.60
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Hello list
I receive today my august news letter;great thanks to the staff...and great
things inside!
I have learn ther that plants of alocasia 'san fernando gabi' will be
avalable in the Annual show..;and i am sure that i will not be there!!!!So
please if someone who go there may help to get this specie,off course i will
send the money in advance .Miami is so long than Belgium that my only chance
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Alta Vista, Ontario, Canada
My home is in Alta Vista, an established leafy residential area of the city. It is one of a few of its kind in Ottawa, built by a local architect. The house...
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Quiet neighborhood. Backyard paradise - 1/3 acre with heated pool, 1/2 basketball court, patio, gardens, backing on to wilderness area. Town ammenities 5 minutes drive. Walk to recreation complex - swimming, ice skating, track etc. Home is 6,000 sq.ft. of living space - living room, family room, dining room, study, laundry/mud room, kitchen, powder room on main floor; very large master bedroom with ensuite, 3 bedrooms and washroom on upper level and 1 bedroom plus ensuite and large games room with pool table, darts in walkout basement.
Our neighborhood is very quiet. We live on a court so there is very little traffic. There is bus service at the end of our road and a park on the next block. We back on to trails and wilderness.
My husband & I are retired and would like to spend 2 months in crop circle country but are not yet ready to sell our home and downsize. We raised 3 sons here and are expecting our first grandchild.
My home is in Alta Vista, an established leafy residential area of the city. It is one of a few of its kind in Ottawa, built by a local architect. The house...
Our apartment is very nice and clean with a beautiful view to Toronto green areas an downtown, but away from congested areas. The neighbourhood is safe and...
Fully renovated two years ago, our loft apartment is on the second floor of a two-storey walk-up on a tree-lined street in Toronto's beautiful Little Italy....


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Generalisation of Levine's prediction for the distribution of freezing temperatures of droplets: A general singular model for ice nucleation
- DOI: 10.5194/acp-13-7215-2013
- arXiv: arXiv:1307.7600v1
Models without an explicit time dependence, called singular models, are widely used for fitting the distribution of temperatures at which water droplets freeze. In 1950 Levine developed the original singular model. His key assumption was that each droplet contained many nucleation sites, and that freezing occurred due to the nucleation site with the highest freezing temperature. The fact that freezing occurs due to the maximum value out of a large number of nucleation temperatures, means that we can apply the results of what is called extreme-value statistics. This is the statistics of the extreme, i.e. maximum or minimum, value of a large number of random variables. Here we use the results of extreme-value statistics to show that we can generalise Levine's model to produce the most general singular model possible. We show that when a singular model is a good approximation, the distribution of freezing temperatures should always be given by what is called the generalised extreme-value distribution. In addition, we also show that the distribution of freezing temperatures for droplets of one size, can be used to make predictions for the scaling of the median nucleation temperature with droplet size, and vice versa. © Author(s) 2013.
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By Linda Arnold
In these times of uncertainty, you may find yourself doubting a lot of things.
Let's face it. In many cases, foundations have been rocked. If you find you're more unsure lately, I'd say that's a normal reaction. If that sense of wavering continues for too long, however, you may want to take stock.
Grandparents take special pride in the accomplishments of their grandchildren. It matters little what the success might be. It could be taking the first steps, speaking first words, riding a bike, learning to read or color or write. What is important is the "first-ness" — the courage to move beyond what is to what can be. What is important is the brave movement into creativity, however it is expressed.
A few weeks ago, I shared some cold weather soup recipes which seemed to be in order, considering the chilly lower-than-normal temperatures we've been experiencing lately. I was also recently reminded that some of the best soup recipes are made with cheese.
By John Nelson
Quick, all you vexillologists: What do Canada, Equatorial Guinea, Hong Kong, Lebanon, Mexico, Norfolk Island and Turks and Caisos Island all have in common? This is too easy. They all nations whose flags prominently feature a plant. This week, we are focusing on the flag of Lebanon, which prominently features a tree, and more specifically, a cedar; and even more specifically, a "true" cedar.
My mom brought me back to my home in Darlington, S.C., from my parents' house in the Triad where they'd taken me so I could lay my beloved cat Kobi to rest about two weeks after the Sept. 11 terrorist attacks.
As soon as I unlocked the front door to my home and felt Kobi's absence, I'd burst into tears.
"Let's get back in the car and you can tell me where the animal shelter is," Mom said. "You need a kitty."
Those who missed the Brunswick County Planning Department Greenway, Bikeway, & Paddle Trail Plan final draft open house Jan. 30 still have time to offer feedback on walking trails, bike routes, paddle trails and resource preservation throughout the county.
By Dr. Susanne Adams
Guest Columnist
Secanda Seifred, counselor to the Brunswick County Early College High School (BCECHS) Firebirds, shares a bit of the history of the Early College High School (ECHS) and the personal reward she has experienced as one of the founders of this North Carolina STEM (science, technology, engineering and mathematics) School of Distinction.
The American Red Cross urgently needs blood donors to make an appointment to give this winter so patients can continue to receive lifesaving treatments.
By Rep. Frank Iler
Last week in the North Carolina House of Representatives, we had sessions on the House floor four days, read in bills but took no votes, and had our first group of House pages on duty.
Copyright © 2017
The Brunswick Beacon is your source for local news, sports, events and information in Brunswick County, North Carolina and surrounding areas.


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Visual areas 17, 18, 19 and 21 of the ferret can be distinguished on the grounds of cytoarchitecture, myeloarchitecture and cytochrome oxidase reactivity, and with transneuronal tract-tracing from the eye. Each visual area contains callosally connected, as well as acallosal, regions. The callosal connections originate mainly from layers 2 and 3 and, more widely, from layer 6. Callosally projecting neurons and callosal terminals are organized in three roughly medio-laterally oriented bands. The posterior and intermediate bands straddle the 17/18 and 19/21 border, respectively; the third band extends along the medial bank of the lateral suprasylvian sulcus. These bands are linked by a variable number of bridges of connections that demarcate acallosal islands. The distribution of callosal connections predicts the existence of vertical meridian representations corresponding to each of the bands and of non-isotropic representations of the visual field within the bridges and islands.
In recent years, the ferret (Mustela putorius) has been increas-ingly used as an experimental animal for studies of development and plasticity of the central nervous system, particularly of the visual system (Dehay et al., 1980; Linden et al., 1981; McConnell, 1985; Chapman et al., 1996; Cramer et al., 1996; Chalupa and Snider, 1998; Chapman and Bonhoeffer, 1998; Pallas et al., 1999). One reason for this interest is the assumption that the organization of the ferret's visual system resembles that of the cat, which has been well characterized. Morover, in comparison with other mammals, the ferret is born at an early stage of neurogenesis (Clancy et al., 2001). Therefore, certain developmental events that occur in utero in other mammals can be studied postnatally in the ferret.
Our long-term goals are to understand the degree of early specification of cortical areas [for a debate see (Rakic, 1988; O'Leary et al., 1994; Ragsdale and Grove, 2001)] and the role of transient cortical projections in the developmental plasticity of cerebral cortex (Innocenti et al., 1977; Innocenti, 1995). Because of normative data already available in several species, the visual system appears most appropriate for addressing these issues. However, the number, location and properties of extra-striate visual areas are unknown in the ferret.
In this paper we have begun a systematic definition of extrastriate visual areas of the ferret using cytoarchitecture, myeloarchitecture and cytochrome oxidase reactivity. Addi-tionally we have investigated the distribution of callosal connections as this approach has provided cues to the location and retinotopic organization of the visual areas in other species (van Essen and Zeki, 1978). These traditional anatomical methods were used to facilitate comparisons with data in other species.
Additional motivation for this work is that the only carnivore whose extrastriate visual areas have been investigated is the cat (Hubel and Wiesel, 1965, 1969; Palmer et al., 1978;Tusa et al., 1979 ;Tusa and Palmer, 1980; Sherk, 1986; Updyke, 1986; Dreher et al., 1996). Homologies have been drawn between cat and primate extrastriate visual areas (Payne, 1993). These hom-ologies, however, need to be tested by studying other species of the same Order.
In a companion paper (Manger et al., 2002) the same areas were defined from electrophysiological recordings and the results compared with the anatomical features described here.
Materials and Methods
This study was performed on five adult female ferrets (Mustela putorius) from Marshall Farms (North Rose, NY) processed in Lausanne (Switzer-land). Fourteen additional female ferrets purchased from a Swedish breeder were processed in Stockholm. All procedures conformed to National and EC regulations.
For cortical/intraocular injections, animals were anesthetized with intramuscular doses of ketamine hydrochloride (Ketalar, 10 mg/kg) and medetomidin hydrochloride (Domitor, 0.08 mg/kg), supplemented with atropine sulphate (0.15 mg/kg). The animals were placed in a stereotaxic frame, and maintained on gas anesthesia (1.5% isoflurane in a mixture of 1:1 nitrous oxide and oxygen). Throughout the experiment the anesthetic level was monitored by measuring heart rate, respiratory rate and the pinch reflex.
At the end of the survival period the animals were euthanized with Nembutal (150 mg/kg, i.p.) and perfused through the heart with protocol-appropriate fixatives (see below).
Tracer Injections
In three animals, processed in Stockholm, one eye was injected with 10–15 μl of 5% wheat germ agglutinin–horseradish peroxidase (WGA-HRP) in distilled water (3 day survival period).
In the other 12 animals the entire visual cortex of one hemisphere was exposed. In the five Lausanne animals, 5–6 × 0.5 μl injections of 30% HRP (Boehringer, Mannheim, Germany) with 10% WGA-HRP (Sigma) dis-solved in phosphate buffer (0.1 M, pH 7.35) were delivered by pressure into the lateral gyrus, with glass pipettes of ~30 μm tip diameter (3 day survival period). Injection coverage was estimated by the extent of labeling in the ipsilateral dorso-lateral geniculate nucleus (dLGN).
In the Stockholm animals we used biotinylated dextran amine (BDA; 5% in 0.1 M phosphate buffer, Molecular Probes) (five animals) or fluorescent red beads (Lumafluor) (two animals). Using a Hamilton syringe, 20–30 injections were made throughout the visual cortex of one hemisphere (2 week survival period). The two ferrets injected with beads also had intraocular injections (above), 3 days before being euthanized. The five animals injected with BDA were also used for electro-physiological mapping experiments (Manger et al., 2002) conducted on the opposite hemisphere. Antibiotics were administered in all cases (Tetramycin, 40 mg/kg, each day for 3–5 days).
Processing and Histology
Four non-experimental animals were euthanized and perfused with 4% paraformaldehyde. Their brains were cryoprotected in 30% sucrose in 0.1 M phosphate buffer (at 4°C), and 1 in 4 series of frozen, parasagittal sections (50 μm thick) were stained with 1% cresyl violet, myelin (Gallyas, 1979), cytochrome oxidase (CO) (Carroll and Wong-Riley, 1984) and 1% toluidine blue.
The five animals with WGA-HRP injections were perfused trans-cardially with phosphate-buffered saline (PBS, 0.06 M, pH 7.35), followed by paraformaldehyde 1.5% and glutaraldehyde 1.25% (or by paraformalde-hyde 3% alone) in PBS, and a postwash with PBS–sucrose 20%. The brains were immersed in PBS–sucrose 30% overnight and then cut parasagittally at 75 μm on a cryostat. Alternate sections were processed for HRP histo-chemistry with tetramethyl benzidine (TMB) as the chromogen (Mesulam and Brushart, 1979), toluidine-blue and CO staining.
The animals with fluorescent bead injections, and those with intraocular injections of WGA-HRP (2 with both) were perfused with 3% paraformaldehyde. Their brains were cut frozen at 50 μm, in semi-flattened preparations (see below); 1 in 2 or 1 in 3 sections were mounted on gel-coated slides and observed uncoverslipped with fluorescent excitation, the other sections were reacted for HRP or CO.
All the animals injected with BDA were perfused initially with 0.9% saline solution (4°C, 500 ml/kg), followed by 4% paraformaldehyde in 0.1 M phosphate buffer (4°C, 1000 ml/kg). The brain was post-fixed overnight in the same solution, cryoprotected in 30% sucrose in 0.1 M phosphate buffer (at 4°C), and sectioned at 50 μm. During the freezing process the dorso-lateral surface of the hemisphere was flattened using a clean glass slide. This caused some distortion along the medio-lateral axis, as the natural curvature of the ferret cerebral hemisphere became flat.
Alternate sections were stained for CO or reacted for BDA. BDA visualization involved incubating sections in 0.5% bovine serum albumin (BSA) in 0.05 M Tris buffer for 1 h. This was followed by incubation in an avidin–HRP solution for 3 h. A 10 min preincubation in 0.2% NiNH4SO4 preceded the addition of H2O2 (200 μl/l) to this solution, at which time the sections were monitored visually for the reaction product. The reac-tion was stopped by placing the sections in 0.05 M Tris buffer. All sections were mounted on gel-coated slides, dehydrated, cleared in xylene and coverslipped.
Analysis of Material
For WGA-HRP material, the injection sites, labeled neurons and trans-thalamic transport in the cortex were documented with camera lucida drawings and photomicrographs. Selected sections were charted with a computerized image-combining microscope (Glaser et al., 1983) (Neurolucida, MicroBrightField Inc., Colchester, VT). The distribution of callosal neurons was correlated with area architectonic borders. In each section, the cortex was delineated by its pial and white-matter outlines, and infra- and supra-granular cells charted.
For 3-D or flattened computer reconstructions, markers were placed at relevant locations (17/18 and 18/19 borders, lateral and suprasylvian sulci, and the crown of gyri). The 3-D reconstructions were obtained from sections digitized with a graphic tablet and analyzed by software developed at the University of Lausanne.
In order to obtain flattened views, we defined in each section a curvilinear axis located at mid-cortical depth. The location of cells and markers were projected onto the axis, in a direction radial to the cortical surface, and the curvilinear axis straightened. This was repeated for every section and the resulting tangential distributions were plotted sequentially, aligned on the crown of the lateral gyrus and separated by the slice thickness.
In the BDA/fluorescent beads material, the labeled cell bodies were examined under light or fluorescent illumination and plotted using the Neurolucida (as above). Outlines of the section, sulcal patterns, electrode tracks and blood vessels were used to align the sections. All sections were aligned and a complete map of the interhemispheric connections was generated for each case.
The three types of rendering used — 3-D 'transparent' reconstruc-tion, computer-flattened reconstruction and tangential sections after manual flattening — have different advantages and disadvantages. The 3-D reconstruction allows visualization of labeled neurons at all cortical depths. The computer-flattened reconstruction disambiguates the depth relations of neurons at different brain locations or in sulci, but it intro-duces distortions of the brain surfaces. The tangential sections allow appreciation of the distribution of the labeled neurons in the absence of major deformations. For this reason it was found particularly useful in combined anatomical and electrophysiological experiments (Manger et al., 2002).
Architectonic Areas of the Lateral and Suprasylvian Gyri of the Ferret
The thickness of layers 1, 3, 4 and 6, neuronal size in layers 3 and 5, and the cellular organization of layer 6 are the cytoarchi-tectonic differences between areas. For myeloarchitecture, differences are in the overall density of staining, the organization of radial fascicles, and the abundance and distribution of tangential fibers. Sections stained with either CO or intra-ocular tracers were useful for determining the extent of areas 17 and 18. Some architectonic differences are subtle and gradual, others are striking and sharper. All are consistent, and allow identi-fication of area borders with an accuracy of ~200 μm. The extent of the various areas is shown in Figure 1.
Area 17
In Nissl stains (Figs 2A and 3A), layers are easily delineated. Layer 2 consists of small, round cell bodies, more densely packed than in layer 3, which is characterized by both small and medium-sized pyramidal cells. Layer 4 is thick and can be subdivided into 4a and 4b, characterized by large granule cells, and 4c, which is thinner and consists of smaller granule cells. Layer 5 has the lowest cell density and contains large pyramidal neurons. In layer 6 the cell density increases again and the neurons are organized in distinct, radially oriented palisades, 2–3 cell bodies thick.
In myelin preparations (Figs. 2C,D and 3E) area 17 is characterized by a high density of thick radial fascicles. These fibers predominate in layer 4, and are mixed in other layers with the less prominent tangentially oriented fibers. In layer 2 there were only radial fibers, and in superficial layer 1, which is myelin rich in comparison to that of the extrastriate areas, there are again tangentially running fibers.
With CO staining (Fig. 2B) layer 4 is clearly identifiable as an intensely labeled band, ~200 μm thick, with a sharp border with layers 3 and 5.
Area 18
Differences in cytoarchitectonics and CO staining can be seen between areas 17 and 18 (Rockland, 1985; Grigonis et al., 1992). Rockland also reported that area 18 is characterized by the dis-appearance of an acetylcholinesterase poor band, characteristic of area 17. Moreover, we identified differences in myelination between these two areas.
In Nissl-stained sections (Figs 2A and 3B), layer 3 becomes thicker in area 18 and can be subdivided into a superficial region, consisting of small pyramidal cells (sublayers 3a and 3b, which cannot be differentiated), and a characteristic deeper sublayer (3c) with prominent medium-sized pyramidal cells, larger than in area 17. Also, compared with area 17, layer 4 is thinner and the palisades in layer 6 consist of rows of individual cells.
In myelin-stained sections (Figs 2C,D and 3E,F), the radial fascicles become less dense than in area 17, and the number of tangential fibers increases in layers 5 and 6. A particularly robust criterion is that superficial layer 1 becomes less heavily myelinated than in area 17, although it remains more myelinated than in further rostral areas.
The CO band (Fig. 2B) is slightly thicker than in area 17 (~300 μm). It maintains a sharp border with layer 5, but its upper boundary is blurred and is located in layer 3.
The anterior border of area 18 is defined by the near disappearance of the CO band in layer 4. The medial-most 1–1.5 mm of area 18 abuts a parietal field characterized by lower CO and myelin. The middle 9–10 mm of the border abuts area 19 (below), and the lateral 1–1.5 mm a temporal region character-ized by particularly low CO and myelin staining.
Retino-geniculo-cortical Projections to Areas 17 and 18
Areas 17 and 18 can be defined by the transgeniculate transport of intraocularly injected WGA-HRP (Fig. 4). The contralateral eye occupies a crescent-shaped territory on the dorsal aspect of the hemisphere, posterior to the lateral sulcus. This territory corresponds to the CO-rich region that characterizes areas 17 and 18 (compare Fig. 4A and B). Regions of more intense and less intense labeling alternate. The latter presumably corresponds to the termination of axons pertaining to the ipsilateral, non-injected eye and the pattern is similar to the ocular dominance pattern described by others (Law et al., 1988; Redies et al., 1990; White et al., 1999). A prominent mediolateral band of lighter staining appears to correspond to that described by White et al. (White et al., 1999), thus its posterior border presumably marks the 17/18 transition.
Area 19
The architectonic features of this area are exaggerated by the local pattern of gyration (Welker, 1990), but are clearly related to the existence of a distinct representation of the contralateral visual hemifield (Manger et al., 2002).
In preparations stained for cell bodies (Figs 2A and 3C) the lamination is indistinct in layers 2–5. Compared with area 18, layer 1 is thicker, and the sublamination is absent. Layer 4 is less distinct and thinner, and the palisades of layer 6 are thicker, being several cell diameters across, as in area 17, but are less orderly.
Area 19 is the least myelinated of all the areas (Figs 2C,D and 3C,G). The radial fascicles are shorter, thinner and less dense than other areas, and layer 1 is almost myelin free. A striking feature of this area is the predominance and density of tangential fibres in layers 5 and 6.
There is very low CO staining, and the CO band in layers 3–4 is weak and inconsistent (Fig. 2B).
The anterior border of area 19 abuts area 21. The medial-most extension of area 19 blends somewhat with area 21 and is bordered by the more heavily myelinated parietal visual areas.
Area 21
In comparison with area 19 (Figs 2A and 3D), layer 4 becomes more prominent, with large pyramidal cells in layer 5. The palisades of layer 6 are one cell wide, as in area 18, but less orderly. Myelin density (Figs 2C and 3H) increases but not to the density of area 18. The radial fascicles remain thin, but their density is higher and they span layers 2–5. The tangential fibers are as numerous as the radial ones in most layers.
Area 21 is CO poor and the band in layer 3–4 is absent (Fig. 2B).
The anterior border of area 21 abuts parietal cortex medially and suprasylvian cortex laterally (Manger et al., 2002), and is characterized by a slight increase in myelination and CO staining, and the appearance of pyramidal neurons in layer 3c. The lateral border of area 21 abuts temporal cortex, which is particularly low in CO (Fig. 4A) and myelin staining.
Distribution of Interhemispheric Connections
Extent of Injection Sites
Five animals received varying WGA-HRP injections in the visual areas of one hemisphere. The largest injection site (Fig. 5A) included most of area 17 dorso-ventrally, most of area 18 and the posterior part of area 19. The dLGN contained labeled neurons throughout its volume. The smallest injection site spared the lateral part of area 17. The dLGN was labeled only in its ventral third. The size and location of the injection sites in the other animals was between those mentioned above. In all animals the core of the injection sites covered the representation of the zero meridian at the 17/18 border.
The injection sites of the BDA or fluorescent beads series were widely distributed over the lateral and suprasylvian gyri, and typically involved parts of area 17, the whole of areas 18, 19 and 21, and beyond. Consequently, the majority of the dLGN and nucleus lateralis posterior were labeled. The different tech-niques provided similar results and will be described together.
Radial Distribution and Morphology of Callosally Projecting Neurons
Labeled neurons were distributed in two laminae, separated by a lamina with few or no labeled neurons. The more superficial lamina (the supragranular zone) corresponds to layer 3, with a few neurons in layers 2 and 4. The deep lamina corresponds to layer 6 (the infragranular zone), but a few neurons were also found in layer 5. The neuron-poor lamina corresponds to layers 4 and 5.
In all animals the neurons were more widely distributed infragranularly than supragranularly. This was particularly true in rostral visual areas, where the infragranular neurons were both more widely distributed and more numerous (Figs 6 and 7). The dendrites were sufficiently labeled to show that the majority of callosally projecting neurons were small or medium-sized pyramidal cells in the supragranular layers, or polymorph neurons in layer 6.
Tangential Distribution of Projecting Neurons
In the WGA-HRP series, the tangential distribution of the callosally projecting neurons was studied with 3-D 'transparent' reconstructions and computer-flattened reconstructions (Fig. 7) from parasagittal sections. The BDA and fluorescent beads series were studied in tangential sections after manual partial flatten-ing (Figs 8 and 9). These three types of rendering provide complementary information.
Labeled neurons were found over a larger territory than that covered by the injection sites, indicating that the visual areas receive callosal projections from both corresponding (homo-topic) and non-corresponding (heterotopic) locations. After injections restricted to the caudal part of the hemisphere, the heterotopic connections originated mainly from the infra-granular layers of the rostralmost areas.
Callosal connections were organized in three bands, partially interconnected by bridges of labeled neurons and separated by acallosal islands (Fig. 1). In the flattened reconstructions of the WGA-HRP series, the different width and distribution of the bands in the supra- and infragranular layers could also be appreciated (Fig. 7).
The most posterior band was oriented medio-laterally on the dorsal aspect of the hemisphere and straddled the 17/18 border. In supragranular layers, this band was 1–2 mm wide, with one-third or less of its width in area 17. The band was broader in infragranular layers and mainly in area 18. Thus, the contribution of area 17 is relatively minor, most neurons being located within area 18 (Figs 6–9).
The intermediate band corresponded laterally to the fundus, and more medially to the posterior bank of the lateral sulcus and extended medially beyond this sulcus. This band was more variable in its thickness and length than the posterior band with which it tended to blend both laterally and medially. It was also somewhat discontinuous in the supragranular layers. This band was seen to straddle the 19/21 border, and in supragranular layers it extended for ~0.5 mm on either side.
A third, most rostral band of labeled neurons, originated from the intermediate band laterally, and ran obliquely along the medial bank and fundus of the suprasylvian sulcus. The connec-tion between this band and the intermediate band was a large patch of labeled neurons in area 21.
The bands were interconnected by bridges of labeled neurons and separated by acallosal islands — irregularly circular regions, 1–3 mm in diameter, devoid of labeled neurons. Typically, the 17/18 and 19/21 bands were interconnected by 2–4 bridges. Therefore, the bridges extend across areas 18 and 19, with the islands centered on the 18/19 border. The posterior and lateral part of the suprasylvian gyrus (area 21) contained 2–3 bridges, separated by one or two acallosal islands. The anterior border of area 21 intersects these acallosal islands. Taken together, bands, bridges, and islands give the distribution of callosal connections a honeycomb appearance (Figs 7–9). Dense callosal connec-tions are also found anterior to area 21, in the posterior parietal cortex (Fig. 8).
In addition to the dorsal region of labeling described above, labeled neurons were found in the ventral aspect of the hemi-sphere outside areas 17 and 18. Regions containing callosally projecting neurons also contained callosal terminals, usually more densely distributed (Fig. 9).
Individual Variability of Callosal Connections
The distribution of labeling differed across individual animals. Some of these differences related to differences in the location of the injections. The labeling was clearly most extensive in the animals of the WGA-HRP series with the largest injection sites. The 17/18 band was more strongly labeled in the WGA-HRP series than the BDA/fluorescent beads series due to the more rostral location of injections in the latter. The BDA/ fluorescent beads series revealed the existence of heavily connected regions rostral to area 21 and medial to the supra-sylvian sulcus. These findings suggest that callosal connections in the ferret, as in the cat (Innocenti, 1980; Houzel et al., 1994), are roughly point-to-point, at least in the supragranular layers.
Residual differences in the distributions of labeled neurons are probably due to genuine interindividual variability of the interhemispheric connections. They concern the precise loca-tion of the callosally connected bands, bridges, and acallosal islands (compare Figs 8 and 9).
Cytoarchitectonic and myeloarchitectonic criteria, reactivity to CO and transport of WGA-HRP injected into the eye allow us to identify three extrastriate visual areas in the ferret. These areas are connected, through the corpus callosum (see below), to corresponding regions of the contralateral hemisphere. The callosal connections relate to areal borders and predict aspects of the retinotopy of the region. There are both similarities and differences in the organization of extrastriate areas in ferret and other species.
Architectonics of Visual Areas in Cats and Ferrets
Difficulties parcellating the occipital cortex of cat using cyto-architecture are documented by the varying descriptions and localization of fields (Gurewitsch and Chatschaturian, 1928; Otsuka and Hassler, 1962;Sanides and Hoffman, 1967). The greatest difficulties were in the identification of areas 19 and 21. These were redefined by Heath and Jones (Heath and Jones, 1971), who relied upon connectional criteria to localize areas 19 and 21. We have used multiple anatomical criteria to distinguish areas 17, 18, 19 and 21 of the ferret. The differences, sometimes subtle, are consistent, and allow identification of area borders with uncertainties of <200 μm.
The architectonic features of area 17 of the ferret resemble those of the cat (Rockland, 1985). In the ferret, the 17/18 border is characterized by a thinning of layer 4, and a thickening and sublamination of layer 3, which also acquires numerous medium-sized pyramidal cells (the origin of the callosal projections). The transition is further evidenced by a thickening of the layer 4 CO band that extends into layer 3 and acquires an indistinct upper boundary (Rockland, 1985). Similar features were described for the 17/18 transition in the cat (Gurewitsch and Chatschaturian, 1928; Otsuka and Hassler, 1962; Sanides and Hoffmann, 1967; Price, 1985). The 18/19 transition in the ferret is characterized by a further thinning of layer 4 and near-absent CO reactivity, as in the cat (Gurewitsch and Chatschaturian, 1928; Sanides and Hoffmann, 1967; Price, 1985).
The myelinated striae of Baillarger, which are used as areal landmarks in the cat and the monkey, were not prominent. The 18/19 transition was characterized by a substantial decrease in myelination, as in the cat (Otsuka and Hassler, 1962). In the cat, differences in the cytoarchitecture of layer 6 between areas 17, 18 and 19 (Otsuka and Hassler, 1962), and in myelination between areas 18 and 19 (Otsuka and Hassler, 1962; Sanides and Hoffmann, 1967), are in part compatible with those reported here. As in the ferret, layer 6 in area 19 of the cat does not show the same orderly lay out of neuronal palisades as area 17 (Otsuka and Hassler, 1962).
The cytoarchitectonic descriptions of cat area 21 are scant and insufficiently detailed and/or contradictory, as mentioned by Heath and Jones (Heath and Jones, 1971). Our description of the ferret concurs with that of Gurewitsch and Chatschaturian (Gurewitsch and Chatschaturian, 1928), who report area 21 as having a thin, distinct layer 4 and larger pyramidal cells in the infragranular than the supragranular layers. According to these authors, cat area 21 appears to extend further rostrally than ferret area 21.
The Visual Callosal Connections of the Ferret
The interhemisperic projections we described probably course through the corpus callosum, not the anterior commissure. The connections of the anterior commissure in the cat (Jouandet, 1982) occupy the ventral half of the hemisphere up to the middle and posterior ectosylvian gyri. The lateral and the suprasylvian gyri are interconnected via the corpus callosum. The origin of the anterior commissure is not known in the ferret, thus we consider 'callosal' and 'interhemispheric' to be synonymous.
For the ferret, as in all species, the majority of callosal neurons are pyramidal cells, although, as in the cat, a few spiny stellate cells are found in layer 4 and polymorphic neurons in layer 6 (Innocenti, 1980, 1986). The callosal neurons occupy two laminae, supragranular and infragranular, separated by a lamina with few or no projecting neurons (Rockland, 1985;Grigonis et al., 1992). With tracer injections limited to areas 17, 18 and 19, the relative contribution of the infragranular layers to callosal projections increases rostrally, in area 21 and the suprasylvian region. This may be because mainly heterotopic feedback projections were labeled in the rostral areas, as reported for the cat (Shatz, 1977; Innocenti, 1980; Keller and Innocenti, 1981).
One important difference from cats is that in ferrets, callosally projecting neurons of infragranular layers predominate numeric-ally in all areas, and occupy more of areas 18 and 19. This distribution is reminiscent of the rat (Olavarria and Van Sluyters, 1985). This difference might be related to the more lateral location of the eyes in the ferret and rat as compared with the cat.
The absence of serial section reconstructions in the studies of Rockland and Grigonis et al. (Rockland, 1985; Grigonis et al., 1992) did not allow appreciation of the overall distribution of callosal connections. We found that the tangential distribution of callosal connections in the ferret resembles that of the cat, where the 17/18 border projects callosally, while the rest of area 17, and islands of areas 18 and 19, are acallosal. This close relation-ship between callosal connections and the 17/18 border is common to all species, including the rhesus monkey (Van Essen and Zeki, 1978) [reviewed by Innocenti (Innocenti, 1986)]. Nevertheless, the qualitative impression is that there are fewer callosally projecting neurons in ferret area 17 than in cat, the majority being in area 18. As in the cat, acallosal islands in areas 18 and 19 alternate with regions traversed by callosal bridges; the bridges merge with another major callosal band that corres-ponds to the 19/21 border. The size and location of the acallosal islands, and of the callosal bridges, vary somewhat across cases. Furthermore, the callosal band corresponding to the 19/21 border varies in length and is often discontinuous. Variability in the pattern of callosal connections has been observed before (Olavarria and Van Sluyters, 1995). It probably reflects true individual differences as the topography of callosal connections matches that of the visual field representation, which is also individually variable (Manger et al., 2002). Presumably, such individual variability of callosal connections is a consequence of their exuberance and modifiability in ontogenesis (Innocenti, 1995).
That callosal connections are more dense in area 18 than in area 17 suggests that they may be particularly strong in regions receiving input from the ipsilateral eye, which is represented in posterior area 18 (Fig. 4) (White et al., 1999). This seems in keeping with a strong involvement of callosal input in the responses to the ipsilateral eye. In the ferret, the callosum might contribute to binocular responses in regions where dLGN input is predominantly from the ipsilateral eye.
In most species, the callosal connections correspond to the representation of visual field locations near the vertical meridian, with visual field periphery acallosal [reviewed by Innocenti, and Payne and Sivek (Innocenti, 1986; Payne and Sivek, 1991)]. Thus, the callosality or acallosality of a cortical site may predict its retinotopy. In most species, the borders between visual areas often correspond to reversals of retinotopic maps around the vertical meridian or periphery of the visual field. The callosal connections of the ferret predict reversals in the visual field representation around the vertical meridian at the 17/18 and 19/21 borders, and around the band in the depth of the supra-sylvian sulcus. Two other reversals around the peripheral visual field representation should occur at the 18/19 border and the anterior border of area 21, which correspond to the acallosal islands.
The alternation of acallosal islands and callosal bridges in areas 18, 19 and 21, and the relation between callosal connections and retinotopy, suggest that the visual field is represented in a complex way in extrastriate areas. Many of the above predictions were met in combined anatomical and electrophysiological experiments (Manger et al., 2002), where we found that the acallosal islands are indicative of regions of peripheral visual field representation embedded into areas of expanded repre-sentation of the central visual field.
Supported by the Swiss National Science Foundation (Grant PNR38, no. 4038-043990) and by the Swedish Medical Research Foundation (Grant no. 12549). We wish to thank Laurence Grollimund and Jenny Hedin for the high quality of their histological work, and Daniel Kiper for having participated in the tracing experiments.


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NT Live: Twelfth Night Cert. 12A
- Running time210 minutes
- GenreTheatre
Tamsin Greig is Malvolia in a new twist on Shakespeare's comedy of mistaken identity.
A ship is wrecked on the rocks. Viola is washed ashore but her twin brother Sebastian is lost. Determined to survive on her own, she steps out to explore a new land. So begins a whirlwind of mistaken identity and unrequited love.
The nearby households of Olivia and Orsino are overrun with passion. Even Olivia's upright housekeeper Malvolia is swept up in the madness. Where music is the food of love, and nobody is quite what they seem, anything proves possible.
Simon Godwin (NT Live: Man And Superman, NT Live: The Beaux' Stratagem) directs this joyous new production with Tamsin Greig (Friday Night Dinner, Black Books, Episodes) as a transformed Malvolia. The ensemble cast also includes Daniel Rigby (Flowers, Jericho), Tamara Lawrence (Undercover), Doon Mackichan (Smack The Pony) and Daniel Ezra (The Missing, Undercover).
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Lahore: Warrants for PPP MNA in fraud case
LAHORE: An anti-corruption court on Saturday issued non-bailable arrest warrants for PPP MNA Tariq Shabbir Khan Mayo, elected from Lahore, for not appearing before the court in connection with a fraud case against him. The court directed authorities concerned to ensure appearance of Tariq Shabbir who is also parliamentary secretary of law and justice before the court by June 8.
The court had issued summons to him on several occasions for his appearance before the court in connection with his alleged involvement in a land grabbing case but he had not turned up. Tariq Shabbir joined hands with some civil servants in 1996 and illegally occupied precious land after preparing a fake registry of said plot.
The original article can be found at The News.


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MLS #1755098 Official listing information! neighborhood - 526 Almaville Rd located in Smyrna TN 37167, priced at $329,900, with bedrooms.526 Almaville Rd Smyrna TN - MLS #1755098 Close
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Season 2 of the Overtime podcast is here! Listen to the first episode with Meg Robichaud now.
Another variation for the new Adventurista website/online shop. We decided to add some color into the site. It's still not final, but we're getting there :)
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Hayden Planetarium Programs
Astronomy Live: The Grand Tour of the Universe
July 26, 2016
Where are we among the stars in the Milky Way? Do we hold a special place in the universe? The Grand Tour will answer these questions as you travel from Earth to the most distant objects in the universe, exploring planets, stars, and myriad galaxies as we fly through the 3D Digital Universe Atlas. Join Emily Rice and Irene Pease to experience the entire observable universe and come to a cosmic understanding of where we are and how we came to be.
More in this Series:
Astronomy Live: Searching for Alien Life in the Universe
February 28, 2017
Sold out - Are we alone in the universe? Jackie Faherty and Emily Rice will investigate this question by touring a nearby solar system and exploring the potential diversity of living conditions on newly discovered extrasolar planets.
Frontiers Lecture: Pluto Revealed!
March 13, 2017
Jeff Moore, geology and geophysics imaging team leader for the New Horizons mission to Pluto, shares what scientists have learned about this distant dwarf planet during that time. Director of Astrovisualization Carter Emmart will present imaging from the mission using the Museum's new OpenSpace software.
Sun-Earth Day
March 18, 2017
Explore the special relationship between Earth and the Sun and learn about the delicate balance that makes our planet the perfect place to call home.
Family Astronomy
March 18, 2017
NASA Solar System Ambassador Laura Jean Checki will be your guide in the Hayden Planetarium as we travel to Arcturus and listen for the roar of Leo the Lion. Young astronomers will learn about the methods and tools used to observe the night sky.
Frontiers Lecture: The Greatest Story Ever Told—So Far
March 23, 2017
Join famed theoretical physicist Lawrence Krauss for a journey through the unexpected fabric of reality, where fact is often stranger than fiction.
Astronomy Live: History of the Universe
March 28, 2017
Emily Rice and Brian Levine break the laws of physics and travel back in time to the Big Bang to understand how it shaped our universe.
2017 Isaac Asimov Memorial Debate: De-Extinction
March 29, 2017
Sold out - Join host and moderator Neil deGrasse Tyson and his panel of experts for a lively discussion and debate about the merits and shortcomings of this provocative and revolutionary idea.
Frontiers Lecture: Our Path to a New Home in the Planets
April 10, 2017
Planetary scientist Amanda Hendrix and science writer Charles Wohlforth highlight the developments and initiatives that have transformed the dream of space colonization into something that could become reality.
Science Throwdown: Sea vs. Space
April 13, 2017
Explore the merits of sea vs. space across a range of judging categories with aquanaut Fabien Cousteau and astronauts Mike Massimino and Don Pettit.
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April 21, 2017
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Astronomy Live: Exotic Skies
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Theology, Representation and Integration of Western Muslim Communities
How should the western countries deal with the threat of internal religious radicalization and Islamic fundamentalism? The search for an interlocutor who should represent religious communities and the emphasis on theological questions are as wrong, says Nadia Marzouki, as the linking of radicalisation with religious practices. Religious practices, even if fervent, do not automatically create dangerous and disloyal citizens.


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fast track
CES 2017: Fastrack Unveils Smart Wearable 'Gesture Band'
Complete with smart features and ergonomic design, Fastrack's new 'Gesture Band' is one of the thinnest smart wearables in the market measuring 9.1 mm in thickness.January 5, 2017, 5:47 pm explore: Tech
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Is social media becoming a fast-track court?August 19, 2015, 9:46 pm explore: India
Punjab molestation: Delhi Commission for Women chief advocates for fast-track court
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Crime against women: Telangana mulling setting up fast-track courts
Telangana Government is "actively considering" setting up fast-track courts to deal with offences against women andApril 5, 2015, 7:22 pm explore: Politics
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Foursquare © 2017 Lovingly made in NYC & SF
Borough Hall Greenmarket
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Carroll Gardens Greenmarket
Farmer's Market
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If you lose your mucus plug?
I didn't. I lost a big blob this morning. It had a tiny bit of blood streaked through it. I wasn't worried at all. Haven't had any contractions or pains, only lots of Braxton hicks.
Talking to a friend tonight she started fretting saying I'm exposing the baby to infection and that I NEED to contact the doctor or midwife ASAP.
So, do/did you call or not?


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Greenleaf Season 1 - Episode 2 "The Baptism"
1 0 Beanie 0.0 11 August 2016
Grace decides to move home in order to pursue the person she blames for her sister's death, but working at the church proves to be more difficult than she imagined. In other events, Jacob takes on more responsibilities at the church as a result of her return; Kevin has doubts about his and Charity's plan to start a family; and the Bishop refuses a senator's inquiries into the church's finances.
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Year: 2016
Language: English
Video runtime: 00:42:14


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DEA Will Play Hardball in Investigating Michael Jackson's Doctors
Mr. Mullin won full federal jury acquittal at trial on a 10 count indictment in a reported case brought by the DEA's Diversion Unit against a New York City doctor - a very rare result. The charges in that case are likely to be similar to the charges many of Mr. Jackson's doctors may ultimately face, including dispensing medication without legitimate medical purpose and prescribing in a manner inconsistent with accepted medical practice.
Mr. Mullin warns that any physician convicted of federal criminal prescribing abuses could face extensive incarceration for each count of conviction, the loss of his or her DEA license to prescribe controlled substances, and significant financial penalties. Mr. Mullin is a federal sentencing specialist, and has argued before the United States Supreme Court on a federal sentencing issue. He also serves in an advisory role to the United States Sentencing Commission.
"The DEA's Diversion Unit, which specializes in investigating doctors and pharmacists, among others, brings extensive resources and experience to this probe. They are tenacious, aggressive and extremely thorough. They will examine every element of this case relating to prescription and other drugs, including the records of the doctors whose names are associated with Mr. Jackson, whether identified on prescription bottles, through pharmacies, or other sources. Pharmacists are also at risk for investigation into their practices, and may soon see DEA agents at their doorsteps," said Mr. Mullin.
Attorney Patrick A. Mullin concentrates in federal criminal defense and complex tax defense matters. He has argued before the United States Supreme Court on a federal sentencing issue and serves in an advisory role to the United States Sentencing Commission. He is admitted to practice in numerous federal district and circuit courts across the country and is a practicing member of the New York, New Jersey and Washington, DC, bars. Mr. Mullin writes and lectures on federal criminal defense, tax defense, federal sentencing, mandatory minimums and related issues.
CONTACT: Patrick A. Mullin, Esq.
Direct Line: 212-639-1600 or 800-780-2889
SOURCE Patrick A. Mullin, Esq.
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(NEWSER) – A "maverick surgeon" claims to have successfully put one monkey's head on another monkey's body, bringing the doctor one step closer to the first human head transplant, New Scientist reports. Sergio Canavero announced his plans for a human head transplant as a treatment for paralysis last year.
Since then, he's been working with scientists in China and South Korea on mice, monkeys, and human cadavers. "It's important that people stop thinking this is impossible," he says. According to Discovery, the head transplant surgery involves cooling both bodies to "a very low temperature" and cutting the neck and spinal cord with an "exceptionally sharp knife" before attaching the patient's head to the donor body. The patient would recover during a four-month drug-induced coma.
Read the full story on Newser.com
Photo: Getty Images


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